LOCUS       DAOSPF010000001       107651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_165_length_107651_cov_7.708140, whole genome shotgun sequence.
ACCESSION   DAOSPF010000001 DAOSPF010000000
VERSION     DAOSPF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 107651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 107651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165_length_107651_cov_7.708140"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK252_00005"
     CDS             complement(<1..212)
                     /locus_tag="PK252_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOT13119.1"
                     /translation="MIDQATIDKIYDAIQIIDVVGDFVSLKRRGANYLGHCPFHNEKT
                     PSFTVSASKGIYKCFGCGKGGNGVTF"
     gene            439..1359
                     /gene="rbsK"
                     /locus_tag="PK252_00010"
     CDS             439..1359
                     /gene="rbsK"
                     /locus_tag="PK252_00010"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOT13120.1"
                     /translation="MGGNLVVIGSTNVDLTMRLDHLPSRGETVGNGDFYQAFGGKGAI
                     QAIAAARCGGKVKFISCVGDDDFGRLAIESLKINGINVDNVFAENGLNTGTALIFTDV
                     QGNNCVGVSPGANESLLPEKIASASAAFENVEMVLLQCEVPYDSIKYVIDASTKYNKK
                     LILNLAPARNIDVSYLSKAHVLVVNEPEAEFLSGFKVNKDSDIEHVAELLLAKGSEHI
                     IITLGARGAYYATSGQNFFVESFSVEAKDSTGAGDIFCGALAVALTEKKSFKEALTFA
                     SAAAAISVTRLGAFPSAPRRAEIEELAAKR"
     gene            1476..2519
                     /locus_tag="PK252_00015"
     CDS             1476..2519
                     /locus_tag="PK252_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13121.1"
                     /translation="MRKLIIVELMTIKYFLGMSLIMLNISCNKYQVSDHCDGRKFFNP
                     EKGHSFGDQIKWMYEIKTIDWPRWIEDIPQPAPPPAVSGGRVRVTYINQATMLIQVGG
                     INILTDPIFSQKAGPVSWLGTKRVRKPGIDIDSLPKIDVILISHNHYDHLDAKSLKKL
                     ASKYQATVFTGLGNKKILQSFGFKKVIEMDWWDKHAINEGTGFVTFVPARHSSGRGLF
                     DKDKTLWGGFVISTANGDIYFAGDTGFGEFANQIQSRFKKFSLAILPIGSYEKRWFMK
                     SQHMNPDDAVRLHLMLNVEQSIGMHYATFNEHPEQAIDAHEKDLREALLIHNVDASKF
                     KVLGFGEGYEMDL"
     gene            2837..5149
                     /locus_tag="PK252_00020"
     CDS             2837..5149
                     /locus_tag="PK252_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13122.1"
                     /translation="MRKVKTILTKALLFGFLMTGSPLAAQLYQAIRGTITDVDSKAPI
                     TGAAIVIQDVEPAMAAISDKDGNFVVPKIPVGRHEVKVSFVGYDAKVISELLVSTGKE
                     LVLNIELKESTTNIEEVVVKAQGKDKPVNAMTTVSSRAFTVEETRRYAGGLDDPGRLA
                     SVFAGVSDGNIESNGIIVRGNSPTGAIYRIEGVEVMNPNHFAGEDLLGGGFVSLISNQ
                     VLDNSDFLTGAFPAEYGNALSAVFDMNLRKGNNENYEHTFQAGVMGLDFASEGPIAEK
                     GKASYLFNYRYSTFGLVQNFLPPGEGLPVYQDLCFNINIPVKQSSISVWGIGGIDGYS
                     FKKEIDDKYQTAIGGIKNKLVLNNGLYINTSLTYNVFVKKNRWFEEYQSQYYPKSKID
                     NIEGSYTFSTFLNKKFNRWITNRSGFSVKNMFFDIDNKYSERAPQPLMQINRGDGDCN
                     LAQAFSQMKITLNEHLMLNTGFHVQYLSLSNQYSLEPRFGARFSINGQHAISAAYGLH
                     SQMALLNVYFIQKTSNTGIENPNKKLGFTKANHFVLAYDWNINKNLRVKIEPYYQYLF
                     DVPVVKDSSFSILNLDLGNDFQETLVNKGKGKNFGIDFTLERFLNNGFYYLATASVFE
                     STYAGGDNIWRNTRYNKNFMANILGGKEWSIDKNNKNKVFGINGRLYFKGGDRISPIN
                     DALSAQEEKVMYESQQAFEKKYPATYRFDVSLSYRVNKPRYSSIWSLQIMNLLYSTIN
                     TYYEYDKVSRTVNKKEEKFVLPSISWKVEF"
     gene            5171..5830
                     /locus_tag="PK252_00025"
     CDS             5171..5830
                     /locus_tag="PK252_00025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT13123.1"
                     /translation="MMKVLKIILIIILLFSMKDVLINGQGKDNSFSKPSRIGVFWHSF
                     FSRVLMGPFYQSYFNQLPLKGNEKVLDFGCGWGAEARILAKKLCKGGRLACLDISPQW
                     IAETKEVLKKYNNVDYYVGDIVVLNIPESSFDAVCIHIVLHDIDVNLRPDIVKAIARI
                     IKPGGHLYIREPLMAERQISASSIESLMNKAGLLERSVKFKRSFWYGEIVEMVFEKKG
                     E"
     gene            5863..7653
                     /locus_tag="PK252_00030"
     CDS             5863..7653
                     /locus_tag="PK252_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT13124.1"
                     /translation="MGLFKKYAFLLTLLCGFAVHLFASADFISEGKVYFTIQKAGSLV
                     TEDNYLKWPVFKDFDWDNLAAGSQLVLAVDSKSVLAAEPAIWIDAYLLEYTIYQGDSV
                     FFSLTGNDRHSQIVPVVRKLSGLVSKPVYIVIPNTAKNIFNRIYSIKIGNYEDLKAEA
                     EAYPTKFFAHQLFLIAIVSIIIFSAILTLLIFFIRWHDKNYALLYYAIAVTMAALSNI
                     DLKVLHFIVHFPFWALNLIILVGRFGEGILFFLFIKSVFELKQRLINIVLFIILLYSG
                     ISVIAYYICPNPVYFSYLSHGYGLLILGILFLYIYYIRQSNVFLKMPLKSLLLFFVSV
                     FLATILFSLLSLFFMYGSHHEVPVYIGVLALSLAMLVLIIGNYLRKLKENNDYVTEAV
                     NQKNELLKLQKANADAQFETLKSQLNPHFLFNSLSTLSSLVASANEPGKAKMFIEEFS
                     HIFRYILDIRSKDVVSLAEELAFVKSYLYLQEVRFGFGLKVKYDIDKTYINCVVPPLA
                     VQIPAENALKHNVVSKERPLLLEIFTENNFLVIKNNLQKKQVTEVESTRMGIANLKER
                     YSLLSGLALEIIETNEHFIVKIPLLEDEYH"
     gene            7640..8404
                     /locus_tag="PK252_00035"
     CDS             7640..8404
                     /locus_tag="PK252_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT13125.1"
                     /translation="MSIIKTLIVEDEELLASSLEQTLAQIEPSVQVVAKLESVVSTVS
                     WLKKNTVDLIFLDIQLSDGLSFDIFEQVNVETPVIFLTSYNHYAVRAFKLNSIDYLLK
                     PLNTEELKNALNKYKKQALKSFDIQEFSRVIGALKDKTAEYKKRFVVYVGDKIKYLDA
                     ENIAYFFIIEGESFMRGFDGNTYSCDYSLDKLQPELDPAIFFRVNRQYIINIKAIKQM
                     FTVSKSTIKLDLTPVPDGDVFVSLSRNSEFKKWLDQ"
     gene            complement(8408..10201)
                     /locus_tag="PK252_00040"
     CDS             complement(8408..10201)
                     /locus_tag="PK252_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOT13126.1"
                     /translation="MNAMLLIIGVLAAFALAYRFYFGFITAKVLTINEHNQVPSKSRY
                     DGQNFVPTSKWVLFGHHFAAIAGAGPLVGPVLAVQFGYFPGFIWILVGAVLAGAVHDL
                     VILTASVRHNGESLAEIARKEVSKLSGSIASLAVLIIIIIALAGLGLVVVNALSESSW
                     GTFTIAATIPIALLMGWWMFVFRKGKTKEATLIGVILLFFAVVFGRNIPGSSLAPYFT
                     FTHHQLTISLAIYGFIASVLPVWLLLSPRDYLSSIMKLGVVAMLALGIIIVMPTLKMP
                     AFTDFIHGGGPIIKGNIYPYLFITIACGAISGFHALVSSGTTPKMLMNEKHIRPIAVG
                     SMLAEGVVSILALIAASSLLPLDYFMINVPAEKLQAILPQLHAMGFTQSDLPSLSAAV
                     GESIAGRTGGAVSLAVGMAQIFSSIPGLKSLMSYWYHFAIMFEALFILTTIDAGTRIA
                     RFIIQETGGKLYKPFARVNHLPSNLLASGIVVFCWAYFIYTGSVSTIWPMFGTANQLL
                     ATLALVIATSFLINIGKKKYIVITIVPMAFVGVTTIIAGILNLKNLFIPQIFNSQTQG
                     QGIVNSLLTLVILISVIIVVIDAVPRWLKKS"
     gene            10312..11712
                     /locus_tag="PK252_00045"
     CDS             10312..11712
                     /locus_tag="PK252_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT13127.1"
                     /translation="MERLFNKEKYIQRRKQLKEKVGFGVIFMPGNFHLSMNYPANPLR
                     FRQDSNFLYFFGLDSPELAGVIDVDNDEELLFGPEFSVDDLIWTGQVEMFDQRAFKVG
                     VSKVFSYSKLERYIKKTVEEKRKIHFLPPYQGEKKIVLSEMLGFPVAKLKEQASDVLI
                     KAVVAIRSKKDADEVDEIEYALNNITAEIYRQCMSVIRPGIKESEFASIAEGYALKHN
                     CRLAYPAICTVNGQYLHNESYDNILKDGQLLLIDAGSESPMHYASDITRTIPVSKKFN
                     TQQKEIYLLVLDMQQKALSLMKPGVPYSFVHKNACEVLVKGMIQLGLMKGNVAEVVEA
                     GAHALFFPHGLGHLLGLDVHDMEDLGEANTGYDETYKRSQQFGTRFLRYGKELHEGIV
                     ITVEPGIYFIPELIDQWKSESRYSQFINYAALEKYKNFGGIRIEDNVLITNDNYKILG
                     NLIPKSVFDVEAACNL"
     gene            complement(11713..14136)
                     /locus_tag="PK252_00050"
     CDS             complement(11713..14136)
                     /locus_tag="PK252_00050"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOT13128.1"
                     /translation="MNEIENNIQPADEAPNIKRYLFKTIINWPLFLISLTIALSIAYL
                     VIRYTEPQYKVSSTIIINEEQKGFESLFPVMGRNLTKRNMFNEIAKIKSYSVINQSLI
                     ELNFEITYNVVGRIKERKVFNQFFQIVLDTSKTNLKYVPVSLTFLNSEEILLEYGEQN
                     KTKKRIRFGNKFENEDFSFWIIKIKDFNPQAAGEDRKLQYSFVINDLNNLTREYIGKL
                     SVTGDEKQGSIITLSSSGPIPDKEAKFVNKVSEIYLRNNLNEKNQAAENAIRFIDNQL
                     NVVGDSLKKNELRLQDFRTNRKVFDILDEEKIISERLIGLQKQKYENGIKLKYCSYIR
                     DYINKKADFSDVMAPGTFGLNDDLLNKSITQLIDLYNQKRELKLSVSETSPAFKILNL
                     KIDNVRKTLVESLNELENTTLYDTRICEEAIQKEEEKLYNLPPIEREYLSIKREYDIN
                     NSIFTFLLQKRAEAGITRASNKADNKVIDEALSTNAVLIKPKRSSVYSTAILLGLAIP
                     FIIIFLMEFLNNQIVDIKDLSRLRKSSIIVSLGHNNKNTETPVFEYPKSSLAESFRGL
                     RTNLQFILREKDDKSILVTSTISGEGKTFCAVNLGIVLSMANKKVLLMAFDLRKPKIH
                     RYLNIPNKTGISSYLSNQAQYEEIIVPTNINNLFFAPAGPVPPNPAELLETENMEKLI
                     KKVKEDFDYIIFDTPPVAVVTDALLISKYASCSLFVVRQNYSTKDVVHLINELAENDQ
                     LPHLNVLINDVKIPGYYGYTSHYGGKYGYKYHYGYSYSDYSYSKGYYSDDDDTPVTFK
                     ERLKRWIGL"
     gene            complement(14145..14927)
                     /locus_tag="PK252_00055"
     CDS             complement(14145..14927)
                     /locus_tag="PK252_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOT13129.1"
                     /translation="MHKRFFFFLLVLVIGASCTRYKDIVYVRDLGKTTSDSLIKTTYT
                     TYKVQASDILYIKIRSSINPTAAEIFNKTSSTSENTYYYNTSNYYLIGYPVDLGGYIN
                     IAVLGDIKVAGLTMKEIEQELQNKAREIVSDAEVVVRLLSFKITVLGEIGSGQKNIMA
                     ERANLFEVFAMSGDILKSGNKHNVLIMRTTPEGIKTFRVDVTDKNLISSPLFYVQPND
                     IIYVEPAKITAVRLTLNELALFITSVSSVVTLSLLVYNLVQK"
     gene            complement(15016..16233)
                     /locus_tag="PK252_00060"
     CDS             complement(15016..16233)
                     /locus_tag="PK252_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOT13130.1"
                     /translation="MGSQAANRLVFSKSGLLPMEEMLEVEKKYKKLVIGIPKENHEVE
                     GRVPLTPEAVEILASQGHEIILESSAGHSANYSDRDYSEKGALLIKDRNSVFQADIVL
                     KIAPPTQEEIHALRGQQILFSSLGITHYDAEYIKSLMAKKVTAFAFENIKDSDGYYPV
                     VRSMNAISGSTSILIAAEYLSNARGGKGVLLGGIAGITPTEVVVVGAGTAAEYAIRAA
                     HGLGAVVKVFDSSIQRLIQLQNILGKPLQTSVFHPQVLAKALKSADVLIGARETFENN
                     IMFYVTDEMVRGMKKGSVIVDLSLMYGGCIESSEYRSLSDPVYTKFGVIHYAIPNLPA
                     RVSRTSSIALSNIFLPILNQIAQSGGINEQLKTDIGLRHGVYIFNGILTNRHIARQLD
                     IPSSDIDLLMAAF"
     gene            16472..16544
                     /locus_tag="PK252_00065"
     tRNA            16472..16544
                     /locus_tag="PK252_00065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16505..16507,aa:Gly,seq:gcc)
     gene            16621..16706
                     /locus_tag="PK252_00070"
     tRNA            16621..16706
                     /locus_tag="PK252_00070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16655..16657,aa:Leu,seq:taa)
     gene            complement(16873..17277)
                     /locus_tag="PK252_00075"
     CDS             complement(16873..17277)
                     /locus_tag="PK252_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT13131.1"
                     /translation="MKNSHVIHLSHVIEHIPDPQNWLLKIKDLLSEDGVAVINIPNKY
                     SLTGKIKLLLVKLKLKNHQWKHEKSKTPDHLYEPTVKSMKWFLEKNGFEILSYYTYSR
                     KDIIAQTLFGKIYNRFLKLGSNSRFYIRIKNN"
     gene            complement(17249..17740)
                     /locus_tag="PK252_00080"
     CDS             complement(17249..17740)
                     /locus_tag="PK252_00080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT13132.1"
                     /translation="MKNKNYVPHAWESVNCPFCGSNNSFVYERFGNELQYNNLKCKIC
                     GVVYFSPRPKYDGKFLEDAYAKYNDFNHITYEYSDRLTKNWDKEISETLSFIKSPNSV
                     LDVGACMGDYLFSAKKYFKHCYGVEVSEKMASFVENKLNLKVFRDNFTSLTFDEKFSC
                     YSS"
     gene            complement(17973..18731)
                     /locus_tag="PK252_00085"
     CDS             complement(17973..18731)
                     /locus_tag="PK252_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT13133.1"
                     /translation="MDFQDFVSVNCVIFGYDFEKLNVLMLDRIMTEPASGKIIFTDKI
                     LPGSHVNFDEDINSAAKRVLFEFTGIDNIELHQFRTFGALDRLNRQDRDVLWLRTIGQ
                     DPYQRVVSIVFYSLVDANKYEWPIQHQKEHHQMYNPGWYPINDLPKEMAYDHHFILDE
                     ALAFLRDGVKYNPLVFELLPEKFSLGLMQKIYETILGRNFDKRNFRRKLLKMPFIVPL
                     NEKQQSVLHKPAQLYYFDRKIYQQVRKNNYDLML"
     gene            complement(18794..19885)
                     /gene="mqnC"
                     /locus_tag="PK252_00090"
     CDS             complement(18794..19885)
                     /gene="mqnC"
                     /locus_tag="PK252_00090"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOT13134.1"
                     /translation="MNLNYLYNKALQLEPLSIDEGAYLFRNAPMEDLMHIGFQLRQKH
                     VPGNIVTWMIDRNVNITNVCVARCKFCNFHRIPGHPESYITTLEQYREKIESMMALGG
                     YQLLLQGGLHPKLGLTYYKELFSALKSMYPMIKLHALGPAEIVHISKMGKMSYSDVLT
                     ELKTSGLESLPGAGAEILVDRVRKAISPGKCSSQEWLDVMHEAHKLNLVTSATMMFGH
                     IETIEERMEHMVKLRETQAKKPEGTYGFTAFIAWPFQDDGTVLQKKMGINNFTDCSEY
                     LRMIAISRIMLTNITNIQASWLTVGKGTAQLALHAGANDMGSIMIEENVVSAAGATHW
                     LDSESMKNTIIDARFTPQLRDQKYSLINE"
     gene            complement(19946..20203)
                     /locus_tag="PK252_00095"
     CDS             complement(19946..20203)
                     /locus_tag="PK252_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HOT13135.1"
                     /translation="MINIQVQISYLPLCSNFNEVIELFLLELNKTENINITTNAMSTV
                     LAGDYHIVFNALQNVIFPFIQKYPSVFNITISNCCDNCTIN"
     gene            complement(20210..20854)
                     /locus_tag="PK252_00100"
     CDS             complement(20210..20854)
                     /locus_tag="PK252_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT13136.1"
                     /translation="MNVFENNEIANEYDAYYQTGQGKKIDEIEKAAILKHLQNIPVKS
                     TFLEVGCGTGHWTKAFANLGFSVTALDISDAMLDIALAKNISHATFIKGDAMNLPFHD
                     NSYDVVVSVTMLEFTQNAKNAIDEMFRVLKKNGLLILGCLNSESVLGKTKDNDPIFKH
                     ARFFSKKDLVELLKKFSNTFIEDCVMLTDSFELVPDTIVNKDIHGVFIVVKATK"
     gene            complement(21022..21264)
                     /gene="atpC"
                     /locus_tag="PK252_00105"
     CDS             complement(21022..21264)
                     /gene="atpC"
                     /locus_tag="PK252_00105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOT13137.1"
                     /translation="MMHLDIITPEKKLFSGNIKLIQVPGTKGPFEILRNHAAIVSTLS
                     SGKIKIVTNDDQKSFFDIRQGVVEVKNNKVVVLATV"
     gene            complement(21282..22790)
                     /gene="atpD"
                     /locus_tag="PK252_00110"
     CDS             complement(21282..22790)
                     /gene="atpD"
                     /locus_tag="PK252_00110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT13138.1"
                     /translation="MADIYGEIIQVIGPVVDVIFDTKGEELPKIHDALEITKENGQVL
                     VIECQQHIGENTVRTIAMDSTDGLRRGTKVKATGAAIRMPIGDQIRGRLLNVTGDAID
                     GIGTLDKKGGYQVHAEPPKFEQLRTETEVLFTGIKVIDLLEPYAKGGKIGLFGGAGVG
                     KTVIIMELINNIAKKYAGTSVFAGVGERTREGNDLLREMIESGVIKYGEAFKHSMEKG
                     GWDLSVVDKEEMLKSQATLVFGQMNEPPGARATVALSGLTVAESFRDGDEKSGGRDIL
                     FFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGVMQERITSTKRGSITSVQAI
                     YVPADDLTDPAPATTFAHLDATTVLSRKISELGIYPAVDPLDSTSRILTPEVVGKEHY
                     QTAQRVKMLLQRYKELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFFVAEQFTG
                     LKGVLVSIEDTIKGFNMIMDGEVDKYPEAAFNLVGTIEDAIEKGEKLLAASK"
     gene            22972..24417
                     /locus_tag="PK252_00115"
     CDS             22972..24417
                     /locus_tag="PK252_00115"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT13139.1"
                     /translation="MKNIALSKTNSIDNNLSIVVIGKKSEPLDLVFLSEAEKNYLKQK
                     EEAGSSFIVINQYIRLVIVARIDTSKAVFEQLEEARGMGNRAQAELIEGKYNQALIYH
                     LFDEKETLLALAEGFLLGAYRFSKYFTKESAKEQSVEQLFVYSQCVREKELTELSNIV
                     DAVYVARDMVNEPVSELNAEKLAQTIEEHCTSAGCKVEILDKAKIEALKMGGLLAVNK
                     GSIDPPSFTIVEWKPDNAVNTQPIVLVGKGIVYDTGGLSLKPTGDSMDYMKSDMSGAA
                     IVAGVMKAAAANKLPLYLVALAPATDNRPSGNAYAPGDVVTISNGTTVEVLNSDAEGR
                     MILADALVFAKKYNPQLVIDLATLTGAASVAIGPYALVAMGTASEEVKRRLNESAFRV
                     FERMVEFPLWAEYGELIKSDIADLKNVGGKYAGAITAGKFLEHFTDYPWMHFDIAGVA
                     FNKSKDSYRVKGGTGFGLRLLYDFLKKEAAK"
     gene            complement(24410..25255)
                     /gene="murI"
                     /locus_tag="PK252_00120"
     CDS             complement(24410..25255)
                     /gene="murI"
                     /locus_tag="PK252_00120"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT13140.1"
                     /translation="MQPIGIFDSGYGGLTIMQEIVKKLPQYDYIYVGDNARSPYGSRS
                     FETVYRYTLEAVKWLFAQDCHLVILACNTASAKALRTIQQNDLPKIAPNKRVLGIIRP
                     VTECIGNFSKTNNIAIFATSGTVSSNSYPIEIEKFFPNVKVEQEACPLWVPLVENGEY
                     DSPGADFFIKKHIDALMSRNNKVDTFVLGCTHYPLLFSKISKYTPEGVKIVSQGNIVA
                     ESLSKYLQNHPEMDEKVTKTGKRYFCTTESADIFNRLASDLYGSPIEGKQISLEKPEK
                     LKYFI"
     gene            complement(25329..25838)
                     /locus_tag="PK252_00125"
     CDS             complement(25329..25838)
                     /locus_tag="PK252_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT13141.1"
                     /translation="MRKYLVILFAAAFISTAGAQTAIKLGHINSQELIEAMPESDSAQ
                     KKIEKLTKDLQSQLETMQVELNNKYQDYLSKRDTYSDLIKQTKENELQELNQRIQTFS
                     QNAEQDLQKQRSGLYKPILDKANKAIADVSKENGFTYIFDLAGGLIIYHAENSTDILP
                     LVKAKLGLK"
     gene            complement(25880..26392)
                     /locus_tag="PK252_00130"
     CDS             complement(25880..26392)
                     /locus_tag="PK252_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT13142.1"
                     /translation="MKKLIILLALTAIPALGFSQKFAFVDTEYILSNIASYKTAQDQL
                     DKLSENWQKEVEAAFAEIDKMYKNYQAEKVLLTEEMKKRREEEIMAKEKEAKDLQKKY
                     FGQDGALFKKREELIKPIQDEIAKAIREFAVDGGYAVIFDSSAGASMLYTDPKMDKSD
                     AILQKLGYKN"
     gene            complement(26443..28914)
                     /gene="bamA"
                     /locus_tag="PK252_00135"
     CDS             complement(26443..28914)
                     /gene="bamA"
                     /locus_tag="PK252_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOT13143.1"
                     /translation="MIKKVLLFLSLISPIFIIAQETSQVQIADFSKPKDYIIDTVTIS
                     GVKFLDQRVLSNMSGLATGAKITIPGNDIAKVIKKFWEHGLFEEIKVSYKPTTSGHVA
                     IDIFLTERPRVSKFNIEGVSKSDKEDLREKLNLKPGSQITENALNEAQIIIKKFYGEK
                     GFFNVSAEIIQKSDTLRKNAVDLTIKISKNQRVKIKDIVFTGNNELKASKLRKSMKKT
                     KRRDWKFWNSSKYILAEYQNDKNNLIDYYNEKGYRDAKIIADSFTVISPKRIILYINV
                     YEGPKYYFRNITWVGNTKYPSEMLSDVLGIKKGDTYNQKLMDKRLQSDDDAVSSLYLD
                     NGYLFFNVNPVETKIDKDSIDLEMRIYEGKQANFNDIIITGNTKTNEHVVRRELRTLP
                     GELFSKSDIVRSVRELATLGHFEPEKIEPVPIPNQANSTVDLQFKLVERANDQLEVSG
                     GWGGYYGFIGSIGVSFNNFSYRNFFKWKEWRPVPSGDGQRLSFSFRTNGTQYRALSAV
                     FSDPWFGNKKPNALSVSMSYTHYTPVRWSSVSQALQSSDQYINTFGLSIGLGKRLTWP
                     DDYFTLQNAINYDNYSFKNYYIISDTSTGSINSISYSIVFGRNSLDQFLYPRTGSNFS
                     IGVQFTPPYSYLNGKDYSKMNAAEKYKWAEYHKWTIKAETYTTIVGNLVLMTRANFGI
                     LARYNNKIDYAPIESYNMGGSGMMTYNITNEEIVPMRGYSDGALTPSPAGRIYDRFTF
                     ELRYPITLKEQATIYAAAFTEAGNAWADARSFNPFDLKRAAGVGVRVFLPMLGLLGFD
                     MGYGFDKINGSVSGWQPQFTMGQQF"
     gene            complement(28938..29693)
                     /locus_tag="PK252_00140"
     CDS             complement(28938..29693)
                     /locus_tag="PK252_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT13144.1"
                     /translation="MNTEGNIDKNRLPLHVAVIMDGNGRWAKKKGNQRIFGHKNAIKA
                     VRDTVEAAAELGIKYLTLYAFSTENWNRPKSEVNALMHLLVATLNSEIETLMKNNVRL
                     QAIGDLKSLPANVYEQLQDSIKKTLSNTGLTLVLALSYSGRWEIIDALKKVAREVKEG
                     HLDPEKIDESIFTNALQAGFMPAPELLIRTSGEYRISNFLLWHIAYSEFYFTDVLWPD
                     FSKKHFYEAIKDFQKRERRFGKTSEQLNSDNVK"
     gene            complement(29694..30485)
                     /locus_tag="PK252_00145"
     CDS             complement(29694..30485)
                     /locus_tag="PK252_00145"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOT13145.1"
                     /translation="MKKACLEVNTNTYKSRFVGTNSTAFARQAIRPLKINFARMKKSI
                     VIVLFMLPVMVFSQQGADLGICIGGASYWGDINYITPFYSPGINLGITYRYNFNPRQA
                     LKIEANYMRLSANDASFDDIYQKSRNNSFALPLYDGAIQYEFNFMPIKFIAQKYYLSP
                     FVSSGIAGAFVMSQSNLKTINFVFPFAFGVRTSIGKNWSLGVQWNFRKLFNDNLDNTE
                     NLVDKKNVNLHTFVNNNDWYNFANIFVTYKIFNSIEECPAYKKEF"
     gene            complement(30492..31259)
                     /locus_tag="PK252_00150"
     CDS             complement(30492..31259)
                     /locus_tag="PK252_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13146.1"
                     /translation="MRKIGLICLLLMVSGILFSQKWKLTRYELMYGVGVSNYFGDIGS
                     TSNNNNLYGIKDIRIFNTRPSFMVGARYKIKQKFHTRVAFSFIMLNGDDSRGSYPQRN
                     YSFNTFGLEQSGQVEYALLSEDRNRTSFAVYNKRGIMYSRSRLGLYAFAGVGGLFYIP
                     FIDHSNPDPSYKGDHFSSGPSYTGIAFGGIMLKTIYSNYLALSLEFGPRFVIGDDLDG
                     LTSKFSKFNDMYYYLNFSVIYRIKTSRKGYPMLFKRY"
     gene            complement(31327..32193)
                     /locus_tag="PK252_00155"
     CDS             complement(31327..32193)
                     /locus_tag="PK252_00155"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOT13147.1"
                     /translation="MNIAIFSKNVNPEHEHLKKMLIEKLIKGNHKVSIHQNLISIIQH
                     NKIGFQVFEKPEQLSKSVDLFISIGGDGTFLESVRYVRDTNIPIAGINLGRLGFLAYI
                     AKNEIEYFVNCINNKTFETEKRSLLAFQSEYNPFSIFPYALNDITIQKSDATLITVTT
                     LINGIELNKYWSDGLIISTPTGSTAYSMSAGGPIVSTDCKAFIISPIASHNLSVRPMV
                     VPDNAIIELSIKSRSENFVATLDAQHSLMTASSVIKISKAPFCINIVRLPGHNFFATI
                     RNKLMWGVDVRN"
     gene            complement(32195..32860)
                     /locus_tag="PK252_00160"
     CDS             complement(32195..32860)
                     /locus_tag="PK252_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT13148.1"
                     /translation="MLAKELISDIVPALRTSDTGIKALAYMDIFRISHLPIVNNSEFL
                     GLISDKDIFDLNMADEPIGNHALSLTRPFVLANQHAYEIIEIFSRLSLSLVPVLDEKN
                     NYLGIVTQYDLLKQMATLFAVNEPGAIIVLEMNINDYTLSQISQIVESNNAKILSTYV
                     SPHPDSTQIDVTLKINKTDLTSIVQTFIRYNYQIKASFLNNNIMDELLDERFELFMKY
                     LNI"
     gene            32922..33635
                     /locus_tag="PK252_00165"
     CDS             32922..33635
                     /locus_tag="PK252_00165"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOT13149.1"
                     /translation="MTSLSVNINKIATIRNSRGGNVPNVVEAAINIEKYGAQGITVHP
                     RPDGRHIKYQDVYDLKKVLTTEFNIEGNPTDSFIKLVSEVVPTQVTLVPDAHDAITSN
                     AGWDTVAHLSFLTSVVKEFKKQGIRVSIFVDPIEKMVEYAAVAGADRIELYTEPYASN
                     YLDNRTDAIASYINAANMAVKVGLDINAGHDLNLQNLKYFAQNIPQLKEVSIGHALIS
                     DALYFGLEKTVHLYLDCLK"
     gene            33971..36766
                     /locus_tag="PK252_00170"
     CDS             33971..36766
                     /locus_tag="PK252_00170"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13150.1"
                     /translation="MRVPLKKVVMALVICFPAVLNASNTKEIDSLISKTETASGVEKA
                     SLLNEIAEKYLPVDAEKAEYYANQARAFSKTSMSKYNEAKSYKISSSSYLSRSRYNEA
                     LKASQEALDIFMKDNRTKEAAETYCQIGQIHKQMLEFEKSMKDYHKAIELYQMGRDSS
                     GVATVYSLIGSLLLKQNQYDEAIKYYTLALKQKQQAQDSMGMVSVYSNLSIVNRDKGN
                     LTAAIENLQKALSILEKKGSKAELANVYNLFGNIYFKKNEYNNAIVNYEKALQIRVQL
                     GNKTEMAGSYTNIGNVYKEMNNFPKAIEYLQKAYDIRKELGNAKAEAIALNFLGNAYW
                     KAKKYSEALSYYLESLKLSLESKESTEISSAYFNIGNIYFELSNYDKALKHYSEALAQ
                     SSSSDDYPKMASIYQSMGNAYQKTGKYTEALECFNNALKLRQKLGDNLQIAISYHSIG
                     GVYTDMNNTQSALKYYKLALDLRKKMNDKVAISNTLNAIGNLYAAVNDKPQALSYFIQ
                     ALQYARDAGYFFNIGLCSRKIGELYTDMGEIEKALPRITESVEIGKKLSNYELLRKGY
                     FALYKLYEKQGKYKEALTNYVLSSQYNDSINVTMTNKQLLDMQLNFEMLSQQSKVHRQ
                     EIEIALLKKDNQNKELNATKNRLLIIVLLILMLFILSLVILYYNRFQMKHKTALMLQE
                     KYDIIESTNQKLKQSEEELRKMNKTKDKFFSIMAHDIKNPLGGMVTITDMARTDFGNL
                     ADDDKKEMFEAINKSSKQLYTLLENLLQWSRSQTGRIPYRPVELQLSEIAENNIELQK
                     ANANKKNIIVINLIENTLKAKGDLEMVTLIMRNLISNAIKFTGEGGRVVISAKQKDNK
                     AEICVEDNGTGISEENLQKLFRIDTQLVTPGTNNETGTGLGLILCKEFVQKNGGEIWV
                     ESEEGKGSRFFFTLPLA"
     gene            36774..39401
                     /locus_tag="PK252_00175"
     CDS             36774..39401
                     /locus_tag="PK252_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT13151.1"
                     /translation="MNRYLTLIAAIFCCSCAFAQKVVDFEDRRFDAWHSIPEGRWELT
                     NQAPIQGKYSLHHLFDNDKSGRDYIFLLYQDMIDWNDTVTWSFKVKHGYNPSSSNNWA
                     ISFTEPDTHVVEPSSDWAIVAGVNQIGSDDMLTLYAINGFQTVKIISSQFNWETKIGT
                     SVGYVKVSRTPDGMWLLWAGNSEDSAILVGQGSFVEPIKATCFSISYEYSSSQDRKLW
                     VDDIHFDMKMIKDIFAPKVDTAYVSGAKEFTLLFDEPVFGAKQSNDFVKALNNEHLID
                     SSIIKDNKIKVYLKDPIACNQHYCFLFTGIQDMHNNTARDTTICFYLFKPKAFDVVVS
                     EIMADPSPSAGLPEFEYLELFNRSDTSINLENWQIHADNKMVKLPKVVLEKDSFLIVS
                     YKNAKGVFDKYGKTVYCLPDVYFLNNGGANISIADSTGQMVSYCWYADSWHTDAIKKK
                     GGWSLEWADKSDLCGGSYSWKASKSKTGGTPGKKNSEGSTKVNDIKIKPSKILVPNDS
                     TLIVSFNKPVDIDAVLDKSLFQIGLSIVSVVNVKFGANFYSLELFLSGHLEKGVTYTL
                     NFSDKICDCNGKPIVKTELSFVLPLEADSNDLVINEVLFNPYAGSTYFVEFYNRSDKP
                     LDIGSLYFARFDTLTNEVYNFCSISEQGSFLMPHKIMAICKDTLLLSKFYKLYDTCVV
                     LSNRDFPSLDNKQGRIGIINRQQQIIDQLNYYEDWHLPFLSSKEGVSLERLSSEALTN
                     DRNNWYSAAGTYGYATPGYANSQKQVNNITNDGIISITPEVFSPNGDGFNDFTTIEIK
                     PSETGVLGAIKIFDSNGNMVRTLLQQQLISENDRFVWNGLSDTNKILPSGIYIVWLRT
                     ISKNGKVDERYKVVTLGNH"
     gene            complement(39408..39920)
                     /locus_tag="PK252_00180"
     CDS             complement(39408..39920)
                     /locus_tag="PK252_00180"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT13152.1"
                     /translation="MDNFQKTDPYKLKDNVFQLLDKEWMLITAGNASKLNTMTASWGS
                     FGVLWKKPVATIFIRPQRHTLSFVEISDMFTLSFLYPEHKDKLSYCGKYSGKDVDKIK
                     ETGLKPIFMPSGAITFEQARLVIECKKLYTAELKKECFNVPQIAEEIYPEGDFHHMFI
                     GEITNCYSKI"
     gene            40013..41314
                     /locus_tag="PK252_00185"
     CDS             40013..41314
                     /locus_tag="PK252_00185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT13153.1"
                     /translation="MKLSIFKYSLWYSIVRLYVKVAYYLYYSKVEVIGRENIPKNRPV
                     IFAANHSNGLMDALAFVCTVNRQVVFLSRADIFSSPFISRVLNFFKILPVYRQRDGIK
                     SVSKNEEIFRIVKEGLCMGKALCMMPEGTHHGGIKLLPLQTGIFRLAIETAKMRGGED
                     VIIIPTGLRYEHPKRFRSKVSIIFGNPISVSELMHLSEANASKLLKDMLTNSLTRLAG
                     YTTLPDSELDVANFALNVPSVKAVKKSEVIKYSNTWVLNRFENIFNNSPIFYKELSRN
                     VSEYNELLLRSKIKPFYLLQFQNSIFLTALSALLLLITLPVFIIGISFHALPLLFAHV
                     LNLKNEDYLFHSSVRFVIWLLWLPFQFTMLTLVLIAIGISISTLLAILFMIVMSGILA
                     LFYLDILRNAVVNIACFLNLRNTLIVKAKQKKIMLKLFSKR"
     gene            41319..42116
                     /locus_tag="PK252_00190"
     CDS             41319..42116
                     /locus_tag="PK252_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT13154.1"
                     /translation="MTGQYKVIWITGVSSGLGRELAVNYAELGVSIIISGRNEAHLAE
                     TALCCKNKGAEILSLPFDMTIDTEIEGAVKLAIAWKGNIDLLILNAGIGQRAYVHDLS
                     MDVADYIMKVNYRSLVYITKLLLPRFLVQKTAHIAVISSVNGLYGFPLRAVYSASKHA
                     LIGFYESLALEMASKGICVTIVCPGRFGSGFSTRALDAFGKPYELLDNTHKNLLSVEK
                     TALKVMSAIARKKYFCFFGGKELILLYLKRLNSNLFFSFTKRLNPKS"
     gene            42120..42845
                     /locus_tag="PK252_00195"
     CDS             42120..42845
                     /locus_tag="PK252_00195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT13155.1"
                     /translation="MLKNLPIEKVLFLDIETVPMSPDFGQLPDIFKSLWDKKSRHFRK
                     EEQTAEEVYERAGIYAEFGKVICICFGTIFQRDGQWHTRIRHFAGDDEKEILFQFAEL
                     LEKLETQGGWRLCAHNGKEFDFPYISRRMLVNGIKLPALLDVTMMKPWETSFLDTMEL
                     WKFGDYKNYTSLELLAALFGIPSPKNDIDGSQVAKVYYEDKDLERIVKYCKNDVLTII
                     QLFLKYRGEPLIENERIDLGVSE"
     gene            complement(42954..43490)
                     /locus_tag="PK252_00200"
     CDS             complement(42954..43490)
                     /locus_tag="PK252_00200"
                     /inference="COORDINATES: protein motif:HMM:NF027596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOT13156.1"
                     /translation="MLLAHHLKQTNIAAYVLYLWQMEDLLRAINFDTSKAKAFLIKES
                     DTNKEDVKNTELWIESLVEMMRNEKVETAGHIQVVINVMNEIEEFHQQLLRNNDGLYI
                     QAFILAQPNIISYKAKSPQSSRPDTHVCLEAMYWLMLARLSKKEASPATVEAIETMSK
                     MLDLLSKRYNEELKKSDS"
     gene            complement(43506..43886)
                     /locus_tag="PK252_00205"
     CDS             complement(43506..43886)
                     /locus_tag="PK252_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT13157.1"
                     /translation="MDERVRIDKFLWAVRIYKTRSIAAEECQKGRVLINGTEVKPSHI
                     VKEGEVIIVRKPPVVHTYRVIEVIKSRVSAPKAKEAIEDLTPEDELVKREIARMAVNF
                     QRDKGAGRPTKKDRREIDRIRDWE"
     gene            complement(43899..44180)
                     /locus_tag="PK252_00210"
     CDS             complement(43899..44180)
                     /locus_tag="PK252_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOT13158.1"
                     /translation="MFKANEYFGGKVASLGCETVEGRTTIGVIAAGEYEFGTSTVEVM
                     TVIAGVMQVKQAGETEWKTYKKFESFVVAKDSKFKVKCDQDTPYLCLYK"
     gene            44341..46272
                     /locus_tag="PK252_00215"
     CDS             44341..46272
                     /locus_tag="PK252_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOT13159.1"
                     /translation="MKNRVIVIVGIFAILMISFFVVRKPIANYALSKVHDKIESRLHL
                     NFKVEKAEFKGLLALKLSNVSITDSLMDTTLYANEILVRPKIWYLFTGKLRLRELHIN
                     QSYINIDSSIVSSVLKHKKDEKTDTLVVEVNYKKTVERIFGTLFSLIPKKVVISDCRI
                     TILPKKITVSVPVFTIVKDKMQGNISVNDDVLKNYFSIHGEMDSHWVSFRLFNDSIEA
                     DHKKVTEMNDRWGMGLMFDSITSLLHYNGVQDGRAEVVSEFSLFNFSVFHKKLSPVPV
                     SFRKLQMSFDFKASEQTLFIDSSSVISFNGFTFSPFVKYSRTDHKYLELGVKNMEFCA
                     DSFFRALPEGLFENIRDIKAEGKLAYRLKVNIILDTPDSVKVLSDLESKKFRIVRYGN
                     ANFSMLNDTFHYTFYDHGVPTYSMLVGQGIGDYTRLDQISDFLKYAVLTSEDGGFFYH
                     RGFNVDAIQEAIAENIKKKRFVRGASTISMQLVKNVFLTRNKNISRKLEEIMIVWMIE
                     NLHLVSKEKMFEVYLNIIEWGPGVFGIKQAANYYFSKKPSELTLEESIFLAAIIPSPK
                     AFKYCFDENKQLKSCYDGYYKMLSNIMLSRNQITVTDTAGLKANVILKGRALEALKKD
                     TAMVVDSVEIDEPFMFLTE"
     gene            complement(46335..46937)
                     /locus_tag="PK252_00220"
     CDS             complement(46335..46937)
                     /locus_tag="PK252_00220"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT13160.1"
                     /translation="MAFELPKLDYAYNALEPHIDALTMEIHHTKHHQAYINNLNNALA
                     AIPSAPDNIDEIIKNISAYPVAVRNNGGGFYNHNLFWKVMSPNGGGVPQGDLADAINK
                     TFGSFDKFKEQLSNAAATRFGSGWAWLVVKADGSLAVTSTPNQDNPLMDVADVKGKPI
                     LGLDVWEHAYYLKYQNRRPDYIAAFWNVINWNEVAKRFKE"
     gene            complement(47153..49054)
                     /gene="dxs"
                     /locus_tag="PK252_00225"
     CDS             complement(47153..49054)
                     /gene="dxs"
                     /locus_tag="PK252_00225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT13161.1"
                     /translation="MSNSPYPLLDQIKFPSDLKKLQVTQLNALCDELREFIIDEMCTN
                     PGHFGASLGAVELAVALHYIYNTPYDQIVWDTGHQAYAHKILTGRKDIFHTNRKMGGL
                     SGFPKREESIYDAFGVGHASTSISAALGITTAAKMNNDKDRRTVAIIGDGSLTGGLAF
                     EGLNQAGHLNSNILVILNDNNMAIDPNVGALNDYLTHITISKTYNKIKTDVWNAMGRL
                     HKFGPKTRSAVQKIENGLKSILMHQGNLFEALHFRYFGPVDGHNIRQLVNILSDLKDI
                     PGPKLLHIMTTKGKGYKFAEENKTIWHAPGKFNKETGEIVASSDNKNKPPRYQDVFGL
                     TILELAKTNKKIVGITPAMPTGCSLNIMMQEMPDRCFDVGIAEEHAVTFAAGMATQGL
                     VPFCNIYSSFAQRAYDQIIHDVALQGLQVVFCFDRGGLVGEDGATHHGVFDMAFLRCV
                     PNIIIAAPMNEVELRNMMYTAQHKNYGPFAIRYPRGAGVTVDWIQPFTEMAIGKGRKV
                     SDGEKIAILSVGHPGNFVQSAIQKLETEHQVKPAHYDMRFVKPIDTDILDEVFSSFGK
                     IITIEDGTIVGGFGSAVIEYAMEKGYKGQIVRMGVPDKFIEHGTVAELHHLCGFDAEG
                     IARKVLEMI"
     gene            49261..49737
                     /locus_tag="PK252_00230"
     CDS             49261..49737
                     /locus_tag="PK252_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13162.1"
                     /translation="MEFKELTESDGWKKFEIKLEKLGALVLLVGLIVHLLNVNPNRPN
                     YLLVVGLSTLTIVYFFCGFKELKSTDYVSSIFFRIYGWGLAITSVSLMFTLMQWPIHK
                     NSLLISIGLLAVSLILALKFRNEENKQQINYIFFIRIGVALVLAGVVYYTKVMGAY"
     gene            complement(49813..50562)
                     /locus_tag="PK252_00235"
     CDS             complement(49813..50562)
                     /locus_tag="PK252_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT13163.1"
                     /translation="MKILIIEDEAPASRRLMKLVKECDANAEIVGVVQSVKEGIEWFS
                     TNPLPDLILSDIQLADDLSFKIFSQLKIKTPIIFTTAYDEYAINAFKFYSIDYLLKPI
                     NQADLQESLNKYKSILQEKQATDFESILKNFINKEYRERFLVYHGDNLIPIMVNDVAY
                     FESEDGVTFLTTKSGKRHIISESLDQLENELNAKAFFRANRQFILSVESIEKIHNFGR
                     QKLKIQVKPASDKEIYVSKLRATQFKNWLDK"
     gene            complement(50596..51657)
                     /locus_tag="PK252_00240"
     CDS             complement(50596..51657)
                     /locus_tag="PK252_00240"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT13164.1"
                     /translation="MLFCEEHLEAEFIKVEKRIKFIAIPLFAVIATLFIAPYPFVFNK
                     LFFYHLASGLVGIIIIWFLIRYIIFSFRRHFPKNHEYLRFALQVLLSCAISISLDYSF
                     MYFNHSYGYEKTGTLCDPSSFDFKNFFIATLLFVILINVIYEGFYLFLKLSHTALENE
                     RYKKESAEARFQNLNSRLNPHFLFNSLNTLTAIVEEDSKKAVSYIQELSVVYRYVLSS
                     QKETWVDLPTEIKFTQSYINLLRMRFEDSLIVNLDICEHHQTYYILPLTIQLLIENAV
                     KHNEISVQKKLEVNIVCSDEFLIVCNNKQKRNIMPSSTKVGLHNINERYRFLANKEVI
                     VEDTETAFTVKVPLVKGMR"
     gene            complement(51785..52210)
                     /locus_tag="PK252_00245"
     CDS             complement(51785..52210)
                     /locus_tag="PK252_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13165.1"
                     /translation="MKNFKFYYNWLNAVMVAMIATGAYMAFFNQSDSFIFNSIVNTPF
                     WGSGNEPDMAARNFQGFIYGLTGAVMIAWCTLMLMVINNAFKKQQLWAWKALLISVLA
                     WFPIDEIISIYYKVYLNAVFNLLLLLIIIIPLMLTRKYF"
     gene            complement(52437..54806)
                     /locus_tag="PK252_00250"
     CDS             complement(52437..54806)
                     /locus_tag="PK252_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13166.1"
                     /translation="MKLNFLMVAILLAATTFINAQRFTQSVRGVIIDKDSKMPLPGVT
                     VVVEGTNPLIGTSTDANGEFVLRQVPVGRQNLRFSFLGYKEASASDIEINSGKELVLN
                     IGMEESVTTLQEVAVVGHRNKTQALNEMNSISARTFSVEESGRYAGSRNDVSRMASNY
                     AGVTNGDDSRNDIVIRGNSPMGLLWKLEEMEIPSPNHFTSVGSGGGPVSMLNYNVISN
                     SDFMTGAFAAEYGNATSGVFDIKLRNGNNQKHEYMFQAGVLGTELMMEGPYKKDYKGS
                     YLVNYRFSTTSILRLMNINFGYAGQADYQDLSYNVNLPVSAKGTLGIFGMGGRSIYKV
                     LYKDKEKEDFSPDSYLNTNTKWISSTGASGISYQHRISDNSYIKTIAGMSGIYEIGRE
                     DSVGLINYEEYPRYRGENYQYKFTLHTYIKSKINAQNKIKVGIIADRYNYSFDTKFDA
                     DANGNLLDRYDGNGAAALLQAYGQWNYSFTDNLLLTTGLHYQYLQLNNDQAIEPRASL
                     KWNFRPQHAFTLGYGLHSQMQPLPIYMVSTNISGNILNTNKNLKFNKSHQFVLGYNWM
                     ITNSTAIKTETYYQYLFDIPVTSNSRPSSFSAINEGMSYTFTTEDSLVNNGTGRNYGL
                     ELTLEKFFGNGYYYLVTASLFDSKYKGSDGVLRNTAFNGKYVFNVLAGKEFKINERSK
                     IMVDFKVTTAGGKRYTPIDYAASQLAKKAVRIESQAYTQQFKDYFRTDIKVTYRFNGL
                     GKAQEVFVNVDNVFNTKNIFAQTYDASKNQLGNIYQLGIFPTFQYKIYF"
     gene            55016..56023
                     /locus_tag="PK252_00255"
     CDS             55016..56023
                     /locus_tag="PK252_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT13167.1"
                     /translation="MKTANNLVKLVLLSVAFSACVPLQRFQDTQDKLNTCQTELDENK
                     QKSEQLGVENTEMKQVIEKNRLQLNNLVKDSMNRMLVIDTLRAQVSNLQLQVAELQNT
                     QESILKGNAKETSRLLKQLQATQEDLKKREAALQKAFQELEEKKNSLNVLNAEITRRN
                     VRLKELENILFRKDSVVNALKAKVSAALLGFEGEGLTVKVNNGKVYVSMQDKLLFKSA
                     STEVDKKGQDAIKKLAKVLEQNPDINVTIEGHTDNVPYKSDERIKDNWDLSVQRATAI
                     VRIIINNSDVAPKRLTASGRGEFSPVGSNKTAEGRAQNRRTEIILTPKLDELFKILES
                     N"
     gene            56180..56422
                     /locus_tag="PK252_00260"
     CDS             56180..56422
                     /locus_tag="PK252_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOT13168.1"
                     /translation="MKLSARNKLKGKIVKVEEGLITAKVLLDLGNDNYISAIISKDAI
                     SDLLLKPGDTAFAIIKSTEVIIGTPCDCGQGKCQCH"
     gene            56425..56757
                     /locus_tag="PK252_00265"
     CDS             56425..56757
                     /locus_tag="PK252_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT13169.1"
                     /translation="MFQVSCKVAISKDGEAFLTLENIQLLIEIGETGSLMAAARSRQI
                     SYMKAWTMVTMLNKTSKYPVVLMQRGGHQGGGAELTEYGRRLLADYLQVEKQVGNLLA
                     QINMELSL"
     gene            56810..57331
                     /gene="tsaA"
                     /locus_tag="PK252_00270"
     CDS             56810..57331
                     /gene="tsaA"
                     /locus_tag="PK252_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOT13170.1"
                     /translation="METDIIYKPIGFIKTPFEKAAEMPVQPQGATGIEGVIELNAEYV
                     AGLSDLDGFSHLILLYHFHRVKGYKLHVVPFMDDKPHGIFATRSPVRPNPIGISIVKL
                     CKVEDNRVFFEGADMINGSPLVDIKPFFPKYDNQQNVRGGWLEAKGDIDITKVKSDSR
                     FDTDKNPGFNRCK"
     gene            57390..58679
                     /locus_tag="PK252_00275"
     CDS             57390..58679
                     /locus_tag="PK252_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOT13171.1"
                     /translation="MRKFYAFMGIGLTSLCCLSKVNGQSTIDAEFRPRTEYRQGFRKP
                     LADTLNAGFVTLQRTRLNADYKGKILNARLSLQDARIWGNSDNKTNASKIELYEAWFE
                     YFITSGFSAQMGRQPLKYDDQRLLSAPNWSNTGLAHDALVLKYNSPFIQVHSGFAYNN
                     TKDTLMNVAYKYTPKQNYKTMGYAWLSKQIYTGTTLSLIGICEGFENKVDYNVVYPRI
                     TYGGNLVYANDSTAWGATLTGYMQQGKDPNKAVGDGYADLKSFFLAAKFSYKIIQKLS
                     ANVGVDYYSGSDAAIDAEKSNTFNRLYGATHSFNGNMEYFVTLPTQGLIDYYGGVTAI
                     ITPKLSADLSGHLFYFDKDFIYKNEKTDKNLGTELDVVLNYSVSKEIAIQGGYSHYFN
                     SGTTMKYYKMQGVEAHPQQWAYVMLTIKPQFYKTPQN"
     assembly_gap    58789..58888
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            58933..59730
                     /locus_tag="PK252_00280"
     CDS             58933..59730
                     /locus_tag="PK252_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT13172.1"
                     /translation="MKRNIKNTWSFVSGTIIGILGGLIGLGGAEFRLPLLAGFFGFAS
                     LDAVIINKITSLVVVSSSLPFRSQSIPWGDVASHWGIIVNILSGSLAGAWLGAHYATR
                     MKTVVLDRFIMVLLIALAFGMIFGHSVMDGGHAAIVQAGTARMVSGAIAGVLIGMVAA
                     ILGVAGGELIIPTLVILFGVDIKLAGSLSLCISLPTMIVAFYRYTQSGAIEIFKREKK
                     FLLWMIAGSIIGSGIGGRLIGIIPSNILVLVLGGILLVSAIKTFKKK"
     gene            59865..60581
                     /gene="modA"
                     /locus_tag="PK252_00285"
     CDS             59865..60581
                     /gene="modA"
                     /locus_tag="PK252_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT13173.1"
                     /translation="MALSVGLHAQKVKVAAAADLRYAMDDIVKAYKAAKPNADIEVIF
                     GSSGNAYTQISNGAPFDIYFSADIMYPRKLKEAGLAITEPRLYAIGRIVLWSSTLDVS
                     KGLGILTEKPNIKIATANPEHAPYGERAVEALKYYKLFDKVEKQMIYGENISQAAQFC
                     LTGNADAGLLALSIVLSPAMSTQGKYYLIDYKTHKPLEQAYVILKQAKDNAEAYAFTE
                     FVATKPAREIFEKYGFTLPK"
     gene            60745..61149
                     /locus_tag="PK252_00290"
     CDS             60745..61149
                     /locus_tag="PK252_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOT13174.1"
                     /translation="MNKLPGKITQMQQSGAVLLVDVDVDGQGFSALLIEPAARPEWLQ
                     AGHTVHLVFKETEVSLAKNLSGLISMRNRMMCNVSKVERGELLSKITLHFRGHSITSA
                     ITTRAVDSLQIAIGDEVEAMVKANEISLMNSI"
     gene            61262..61942
                     /gene="modB"
                     /locus_tag="PK252_00295"
     CDS             61262..61942
                     /gene="modB"
                     /locus_tag="PK252_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOT13175.1"
                     /translation="MDAIFAETLWITCKFAVFTTLILIVVGLPLSYWLAYSKFRFKPL
                     VEALVSMPMVLPPSVIGYYMLVAYSPRHWFGGWLLDTFNVRLAFTFEGILLASVIFSM
                     PFMVQPLQNGLRALPDSLREASYTLGKSKMVTFLRVLVPNIKPSIITAIALTFAHSIG
                     EFGIVLMVGGNMPGETKVASIAIYDEVQALNFAKANQYALILFSISLLLLTIIYSVNK
                     KHDPWKAM"
     gene            61927..62817
                     /locus_tag="PK252_00300"
     CDS             61927..62817
                     /locus_tag="PK252_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT13176.1"
                     /translation="MESNVITLEVEKMMLTANGKLNLSVDTRIQTGEFVALFGLSGAG
                     KTTLLRILSGLMKPDKGLVKFGDTVWFDSSRGINIPPQERNISFMFQDYALFPNMTVE
                     ENIHFAQPMKDNATALELLSIFGLSEFRKRKPGGLSGGQKQRVALARALARKPQLLLL
                     DEPLSALDSGMRLTLQNEIYKAHQLLGATTIMVSHDLGEVFRLASHVLCIKKGQISKE
                     GTPDEVFSDSSISGKVQITGQVARIAKQDTVNIVTVIAGGSQIIKVIAFDSDMEALNI
                     GDNVLVFTKAFNPMLVKLMA"
     gene            complement(62814..63599)
                     /locus_tag="PK252_00305"
     CDS             complement(62814..63599)
                     /locus_tag="PK252_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOT13177.1"
                     /translation="MVSKQYAYIHHPPSPEKLYKTAFHINFGEVDASGKATITSLSNY
                     LQEAAWQHAMQYQAGYREMYKNNTAFVIHKMLLKLRCLPSWNDYIIVETWLRPPQQLH
                     ALREFTIRDAKLNLIAAASIQYLAINTQTRKIEQFPTVMETAYLNAEFLDEEPANISI
                     AKNNYSNTIHKVRPSDLDLNQHANNGKYLQWVFDYMPFETNCQKHLCCVKINYKHEAR
                     LNDELNLFFIEKNNLIKIHGFNQTAGKTSFTAELKYKCLPEKN"
     gene            complement(63589..64059)
                     /locus_tag="PK252_00310"
     CDS             complement(63589..64059)
                     /locus_tag="PK252_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13178.1"
                     /translation="MENNILIRRATKDDIPAMVKLLEQLFSIEADFNFDADKQTKGLR
                     MLIKAYPKACVMVATIDEKVVGMLQGQVSISTAEGALSMMLEDMVVESGYRKKGIGKK
                     LIDEMQDWGERIGVVRMQLLADITNTNALDYYSHIGWKQTRMFCQRKYTKLYGK"
     gene            complement(64081..65268)
                     /locus_tag="PK252_00315"
     CDS             complement(64081..65268)
                     /locus_tag="PK252_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13179.1"
                     /translation="MKKNTSIFSCSSPLPPGEGLEVKHPYLIDSTLRDGEQAPGVVFG
                     LNEKITIASMLDKIGIEELEIGSPFISEHDLIMAQKLVSYKFSFRCSCWSRAKIEDID
                     AAAKTGATGINISYPVSDIQLLSMGKDRQWVLGSMPEIVSYAKKQFQYVSLGAQDASR
                     ADKKFLHSYLNIAKKLGVYRVRIADTVGCLNPLTTRSLIKGVVSKVKDSNLQIEFHGH
                     NDLGMATANSISAIQAGADCVSTTVNGLGERAGNAALEEVIMAMKISMGIQVPYSTEH
                     LNELCTFTAEASERKLPADKPIVGEMTHRHESGIHTNCMLRDRRAYEPFDAREVGKSG
                     SSFVFGIHSGSNALNSLLEKNSINLDKQKLQELLKKVKTTAVCKRRSLTDAEVLLLSN
                     SYR"
     gene            complement(65416..65721)
                     /locus_tag="PK252_00320"
     CDS             complement(65416..65721)
                     /locus_tag="PK252_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT13180.1"
                     /translation="MKKPAYHILVCNSYRVAGDAQGACNKKGAAQFIQYIMEGCSDRG
                     IDAAVSSTGCLNVCAQGPVMVVHPNNFWYGNISEDAIDEILDALENNELVEKYSIAD"
     gene            complement(65740..66063)
                     /locus_tag="PK252_00325"
     CDS             complement(65740..66063)
                     /locus_tag="PK252_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT13181.1"
                     /translation="MKIAFTSSDGIAIDKHFGKADKLYIYDVSDGFINFIGIRRITAY
                     SSKENNHEINPEALEHIVSALHDCSLIYTASIGETPKKYIQSRGIHVKILTETIYSVF
                     LKYKY"
     gene            complement(66175..67455)
                     /gene="nifB"
                     /locus_tag="PK252_00330"
     CDS             complement(66175..67455)
                     /gene="nifB"
                     /locus_tag="PK252_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOT13182.1"
                     /translation="MSNTATTEEKLHRHPCFDEEAKGKYGRVHLPVAPNCNVQCNYCN
                     RKFDCVNESRPGVSSAILAPSQATEYMRLISNKGVEIAVVGIAGPGDPFANVNETFET
                     IKQVKTVFPDMLYCLSSNGIDVAQHIDKIVELGVSHVTITMNAIDPEIAAQIYSWIRY
                     NKKIYRGIEAGKLIIERQTEAIKLLKQHNITVKINSIILPGINDHHIVEVAKYVASLG
                     ADLMNCIPVHPNKDTAFEDIEEPSKQMVHDIQQQISAYIKPMKHCARCRADAAGLLGK
                     DIADTVTLLKEAANKPLFATENRPYVAVASHEGILVNQHLGEAKDFLIFANTKEGYIH
                     VDTREAPKAGLGDIRWIQLAKLLSDCNTILVNGAGANPSSMLKSMGIKIIQMNGLIEA
                     GLDAIYKGASLNGLTACKPFKCGEDCSGSGGGCG"
     gene            complement(67497..68897)
                     /locus_tag="PK252_00335"
     CDS             complement(67497..68897)
                     /locus_tag="PK252_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOT13183.1"
                     /translation="MKTTATYKENTTAAATTNACKVCSPLGASVAFKGIKGCVPLIHG
                     SQGCSTYIRRYLISHYKEPVDIASSNFSQDTTIFGGHRNFNKGIDNIIRQYKPEVIAI
                     ASTCLSETIGEDIKGLISAYKAQHKDEPLPHFVAVSTPSYQGTHMDGFHEAVASIVAA
                     FAERRFKRSHINLFPGFVSPEDIRHLKEILEAFGIEAIILPDYSESLDNPNWEEYHRI
                     PPGGTSVNDIKKTGSAQASIEFGHILNLGNINNSVNDSKVNPTGAAFLQNNFGIPNMR
                     IGMPIGIKATDLFFEKLEGIAQIKTPEKYTKERGRLIDAYIDGHKYVFGKKAVVYGEE
                     DFVIAMMMFLDEIGIEPVLCATGGESGKAQDRIRQLTGNSKLTILQGMDFETIASLSR
                     ELKPDIMIGNSKGYYIARELNIPLVRVGFPIHDRMGGQRILHLGYKGTTQLFDSICNA
                     LIEYKQNNSPVGYKYM"
     gene            complement(68947..69255)
                     /locus_tag="PK252_00340"
     CDS             complement(68947..69255)
                     /locus_tag="PK252_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13184.1"
                     /translation="MDAFLEQYIETWVQKEPFKKRIESFFVSFSIYDASGNKYINPVR
                     GLGRKTIEKKEDKLLVAKRKMKITYTKGFMPFDTPQSEIEISQEENAPYKYHRGLKVS
                     "
     gene            complement(69301..70698)
                     /gene="nifE"
                     /locus_tag="PK252_00345"
     CDS             complement(69301..70698)
                     /gene="nifE"
                     /locus_tag="PK252_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HOT13185.1"
                     /translation="MEKENKNIYLLGREKQIATAGDANFDMECSKASEAGSVSQRACV
                     FCGSRVVLYPIADALHVVHGPIGCASYTWDIRGALSSGPQLHRLSFSTDLREEQVIFG
                     GEKKLYAALTTLIDKHKPAAAFVYSTCIVGVIGDDIEAVCKKVSAEKGITVLPVHAEG
                     FNGTKKDGYRVACEALSKLIGTSEQRNKVPFSINILGDFNLAGELWMLAEYYRKIGIN
                     IIAQVTGDGRVEDIRRANTACLNVVQCSGSMTWLAKDMKEKYGIPYIRVSYFGIEDMS
                     NALYEVARFFDDESKMDAAKLLVATELKKLLPALKEYKEKLTGQKAAIYVGGAFKAIS
                     LVKALRLIGVQTVVIGSQTGNKDDYNLIKDICDEGTIIVDDSNPIELQHFIREKNATI
                     FIGGVKERPIAHKLGIGFCDHNHERKEALAGYEGMLNFAKEIYATAMSPVWQFVRKSP
                     SQEEPRIKNQEQRLK"
     gene            complement(70847..72229)
                     /gene="nifK"
                     /locus_tag="PK252_00350"
     CDS             complement(70847..72229)
                     /gene="nifK"
                     /locus_tag="PK252_00350"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HOT13186.1"
                     /translation="MLLRHTTDKVIDRQALTINPAKTCQPIGAMYAALGIQGCLPHSH
                     GSQGCCSYHRSTLTRHYKEPVMASTSSFTEGSSVFGGQSNLLQAIETIFSTYDPEIIA
                     VHSTCLSETIGDDLNQILTKAREEGKVPKGKHVIHCNTPSYVGSHVTGYANMLLGMTS
                     YFAQHTGISNYQVNLISGWIEPCDMREIKRIAKKMQANTMMFPDTSGILDGPLNGKYT
                     FYPKGGTTIEELISAGDAKFSLGIGQWATEEAAKKLEYKCKVKYDICDVPIGLQATDR
                     FITALSRYANVKVPQSLTNERGRLLDMIADMHSYLYGKRVALYGDPDWLIPMTEFLVM
                     LDMKPVYIVSGTPGKLFESRMKEILGEKVPEAKFKNGETADMFLLHQWIKQEKVDLLI
                     GNTYGKYIARDEDIPFVRYGFPILDRVGHSYFPTVGYHGGMRILEKILGALMDRIDRD
                     APEEKLELVM"
     gene            complement(72329..73960)
                     /gene="nifD"
                     /locus_tag="PK252_00355"
     CDS             complement(72329..73960)
                     /gene="nifD"
                     /locus_tag="PK252_00355"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HOT13187.1"
                     /translation="MKVEEKILPDPSALKKEILSKYPSKIAKKREKQLIINDPEKDQP
                     IQANVRTIPGIITQRGCTYAGCKGVVLGPTRDIINLVHGPIGCSFYAWLTRRNQTKPP
                     SPDAPNYMMYSFSTDMQDSNIVFGGEIKLKAAIREAFEIFKPKNIGIFSTCPVGLIGD
                     DVHAVAREMKEELGINIFGFSCEGYKGVSQSAGHHIANNGVFKHVVGLNDTPVEGKYK
                     INLLGEYNIGGDAFEIERILEKCGITLISTFSGNSTIDSFEQAHHADLNVVMCHRSIN
                     YLAEMMETKFGIPWFKVNFIGAEATAKSLRKIGQYFEDKALIDKIEEVIAEEMPPVKE
                     ALEKIKPRTKGKLAMLFVGGSRAHHYQELFNELEMETVSAGYEFGHRDDYEGRKVIPT
                     IQVDADSRNIEELHVEKDETRYRPRKTEEQLKALEAKGLEISGYSGMMPSMKKNALVI
                     DDVNHWETEKLIEFYKPDVFCAGIKEKYVVQKMGIPLKQLHSYDYGGPYAGFAGAINF
                     YKDIDKLTGATVWKYLQAPWETHKQVEANFVIDVL"
     gene            complement(74061..74426)
                     /locus_tag="PK252_00360"
     CDS             complement(74061..74426)
                     /locus_tag="PK252_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT13188.1"
                     /translation="MKTIVAIIRMKKVNATKAALAEAGLPSFMGSGEVQGRGRGLGVG
                     PTYEENKKNPDYVHEEHRLKTKRMIQLTVTDEKADLAIQTIIKANQTGNSGDGKIFVL
                     PTAETIQIRTGESGDSVLD"
     gene            complement(74567..74893)
                     /locus_tag="PK252_00365"
     CDS             complement(74567..74893)
                     /locus_tag="PK252_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT13189.1"
                     /translation="MLQIRAIIRPEKAVEVMKALLENGFPAVTKSQVYGRGKQRGLKV
                     GNITYDELAKTSLMIVVNDADKSIVVETILNAARTQPKGQFGDGKIFIIPVLEAWTIS
                     NGKKEL"
     gene            complement(74958..75782)
                     /gene="nifH"
                     /locus_tag="PK252_00370"
     CDS             complement(74958..75782)
                     /gene="nifH"
                     /locus_tag="PK252_00370"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HOT13190.1"
                     /translation="MRKVAIYGKGGIGKSTTTQNTVAGLVEMGKKVMVVGCDPKADST
                     RLLLGGLAQKTVLDTLREEGEDVELDDVVKSGFKGVRCVESGGPEPGVGCAGRGIITS
                     VNLLEQLGAYDESQEIDYVFYDVLGDVVCGGFAMPMREGKAQEIYIVCSGEMMAMYAA
                     NNICKGIVKFAEAGDVKLGGLICNSRKVDNEFQMIEELARRLGTQMIHFVPRDNMVQR
                     AEINRKTVIEYDPTHPQADEYRQLCRKIDENKMFVIPKPLAIEELEELLINYGILN"
     gene            complement(76780..78321)
                     /locus_tag="PK252_00375"
     CDS             complement(76780..78321)
                     /locus_tag="PK252_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT13191.1"
                     /translation="MKTGEKKYTELSFLYDISKELQKVKHIEESIKPILKHLADYCGI
                     SCCSLTILNADLHHVILEEYHGLKSEEVSNLRGKLEKVSKTIIETGEPMLLPKMSHTP
                     TFIKSSRDLVDFSYEISMICVPIKAAGKVLGFFAFSLEYYNDISFKLEFRILNIIGSM
                     LAHSIIATQENAYELALLRQENLKLQDQLNQAIINTEIIGNSSAMQSVFELIQKVSKT
                     NATVLLRGESGVGKELIANAIHYNSARKDKPLIKVNCSALPDTLIESELFGHEKGAFT
                     GADGLRKGRFELADGGTIFLDEIGDLPLPTQVKILRILQEREFERLGGSKTLKIDVRI
                     ITATNRNLEEMITKCEFREDLFYRLNVFPIYIPSLRERRNDIPILVNHFIAKANKRHG
                     LKIKRISTSAIDLLMMYHWPGNIRELENVIERAAILSIDDVIRSNNLPPTLQTAESSK
                     TKMEGTLEDIVANVEKQVIIDNLISTHGCTFETAQKLGITERILRLRLHKYNIEPKRF
                     KKVSK"
     gene            complement(78412..78729)
                     /locus_tag="PK252_00380"
     CDS             complement(78412..78729)
                     /locus_tag="PK252_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13192.1"
                     /translation="MEIPFRPLGKAMNIVASTGLDVTYVYDDLVFADNSVFILQFDNK
                     DPDRMLLHFNADCDEVAREKLTNILIEASNKENVAITYTGTFRLKENQKSEKIEIEFD
                     FIN"
     gene            complement(78736..78990)
                     /locus_tag="PK252_00385"
     CDS             complement(78736..78990)
                     /locus_tag="PK252_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13193.1"
                     /translation="MSINNVGVLFRQMDERDELRQSIYRCKNKGELVKILDMHQMSFT
                     IDEFEEAANVFHAKCQSQEEADWLFGKVNWFRFLYYSLAN"
     gene            complement(79012..79398)
                     /locus_tag="PK252_00390"
     CDS             complement(79012..79398)
                     /locus_tag="PK252_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13194.1"
                     /translation="MKIGIPLKNSNELAQDFAHSKLIYIIDLANDRQQTIELENTHQE
                     AQLLFNSLVNNKISHVVSPFYSFMSLRVFKENNIVTCKAHSTSLEENISRCKAGQIRA
                     FDIYESLLTGECAKDCLSCGPESDCH"
     gene            complement(80154..80540)
                     /locus_tag="PK252_00395"
     CDS             complement(80154..80540)
                     /locus_tag="PK252_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT13195.1"
                     /translation="MSSPNYSSRTFLIVEDDPVSAMYLKDLLDKTGAKVIQVGTASDA
                     IYATHENKDIDLILMDIQLPDMSSWQATRIIKKVKKDLPVIIQSAYAFDSYITKSKEA
                     GCDCFLAKPIDPELLYANINGFLSKE"
     gene            complement(80565..81128)
                     /gene="frr"
                     /locus_tag="PK252_00400"
     CDS             complement(80565..81128)
                     /gene="frr"
                     /locus_tag="PK252_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT13196.1"
                     /translation="MSEEVQLYMDDAKDKMNKALVHLESELLKIRAGKASPAMLAGVM
                     VDYYGSRVPITQVANVNTQDARTLVIQPWEKKLIDALEKAVFAANLGLTPVNNGEVIR
                     ISIPPLTEERRQGLVKQVKHEGENAKVSIRNARREAIEEIKKLQKQGIPEDEIKKAEE
                     DMQKTTDHFSKKVEEVLQKKEAEIMVV"
     gene            complement(81158..81868)
                     /gene="pyrH"
                     /locus_tag="PK252_00405"
     CDS             complement(81158..81868)
                     /gene="pyrH"
                     /locus_tag="PK252_00405"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT13197.1"
                     /translation="MLKYKRILLKLSGEALMGQQGHGIDTARLEEYCKQVSETAKEGV
                     QIAIVIGGGNIFRGMRGVDKGFDRYKGDYMGMLATTINGLAIESMLESLKTKVKLFTA
                     FSMHPVGEAYSKSKAIEAMNNGNVIIITAGTGNPYFTTDSASALRAVELEVDALLKGT
                     RVDGVYTADPEKDPKATKYDELTFAEAYSKGLNIMDLTAFTLCSENNMPIIVFDMNKL
                     GNLKKLISGEKIGTLIKK"
     gene            82534..83070
                     /locus_tag="PK252_00410"
     CDS             82534..83070
                     /locus_tag="PK252_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT13198.1"
                     /translation="MDERILISKCISGNEKAQKLFYDTFANQMFRLCLRYTGDYHDAE
                     DVMIIAFHKVFKNIRNFEYRGDGSLKRWVKTIMINESLMFLKSSRKFMFETIDNTHIQ
                     IADGIENTIDAEQIYQLILKLPDGYRTVVNLFIIDGLSHEEIANQLGISVNTSKSQLS
                     RARALLKEQLFKLEENGR"
     gene            83060..83689
                     /locus_tag="PK252_00415"
     CDS             83060..83689
                     /locus_tag="PK252_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13199.1"
                     /translation="MEDNFDKYLKQKMDAVSDAPISEHLWNKENTWQKIALGIKPQGK
                     SVSIRWFYAAASVLLVVLIGSLVFYNHLSKQMSQLRAENKRLGEIAINRNNTSGPVEK
                     VLIKTDTVIKELIKKENVQNTLFIHDTVVIVKASEEQLAVNAMPDKDSVSASSTNEPP
                     KKSKLHFVINNEIFVENEKSKVKLIGSKELTQKDPERTKQDASIKFAFN"
     gene            83906..84934
                     /locus_tag="PK252_00420"
     CDS             83906..84934
                     /locus_tag="PK252_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13200.1"
                     /translation="MKTLKKSAFIALFVFISLSITAQGFPEKLILLTSSDVKMVIETR
                     EILDIPHEKKQDTLFMNLLSDISKVQDSLTRLNVPLQIFYSTMDKDSRSMKVANNAET
                     KAEFIFKGASDTVRSNFFNYKIECDLNWNNKLILYTNNLSELGELKNTSFSDIVQQAQ
                     ADIALRKIPSYKAKTCLYKINHNQLDATSADIKQQGTFIITPGMDFGLSYLNDRFMPN
                     ISMSMAYVFSPKNEKKCMLGISGEIFYNMDTAEAYKMQGNSFVDVFFHAKNPYSPIYY
                     ASDFRFFIGYLVHREGVVFPKNSWRFGLDMPAGKNFRINYVVYLNDFGSNNKNKGLFE
                     IGIKYKFF"
     gene            84989..86452
                     /locus_tag="PK252_00425"
     CDS             84989..86452
                     /locus_tag="PK252_00425"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HOT13201.1"
                     /translation="MINLLIHHWKEKQRSPFWQKSIALNIILGILCLYLLLNVLALSF
                     FADKIIQETYKGRDVVETFTGLLFYYFSFDLVIRFLFQQLPTLSIQPYLTLPIKKSKL
                     LHYPLIKSGSSFFNIVAILILLPFFIKVVCATHSFPFCFAWIVALFSFIIANNYLNFL
                     LKKYFAKRPLLILLLLAFVGLFIFFDIAKIVSCSSYFSAILIYLANTPFLIIIPVALA
                     ALTYYLAYFLLKKNSYVEDKLTDKRRNADSFLFLKRFGEIGDLVHIEIKMILRNKRPK
                     SLLYFSLIFLAYGFMFYKKQNLDNYLLLSFVGVLMTAMFALNHGQFMFAWESSFFDSF
                     MTKKISPYNYVKSKYLFFTVLCLLSFIVSLPYALISYKIGIINVSLLLYNVGVSSFIL
                     IFFGTYNSTRIDLGKSQFMNYQGIGATQFLLILPIMGIPVLVYLLFNLFDIPQYSFYV
                     LGILGIIGIAFNKYLLQLVTDQFIKRKYKMAYGFRQK"
     gene            86459..87157
                     /locus_tag="PK252_00430"
     CDS             86459..87157
                     /locus_tag="PK252_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT13202.1"
                     /translation="MLNMIEINDLQKVYGKKVALSINRLNIREKEFVGLVGNNGAGKT
                     TMLSLILDLIEPTKGFVKSKGMMVSESDDWKNYTGSYLNEGFLIPFLTPFEFLEFIGN
                     LHGKNRAEVTNFLKENNSFFTEEVSSKKYNRELSAGNKNKLGILASFLPEPEILILDE
                     PFSNLDPSSQSWLKSKLKKLNEEGVTILISSHDLKHVTEICSRILLLENGTIIKDTNT
                     NTETLVELENYFNV"
     gene            complement(87297..87578)
                     /locus_tag="PK252_00435"
     CDS             complement(87297..87578)
                     /locus_tag="PK252_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT13203.1"
                     /translation="MTLDEFKDKHYGVKGTKTRDELDDGYENFKIGALLHEARLEKGL
                     TQAELAEKVGTTKSYISKIENNIKEVRISTLQKIVQLGLGGQLELSIKL"
     gene            complement(87583..87933)
                     /locus_tag="PK252_00440"
     CDS             complement(87583..87933)
                     /locus_tag="PK252_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT13204.1"
                     /translation="MNSKRKYRTAIFYKDYFESFFIKQSERVKAKIIWTIELIEELER
                     VPEIFLKHIESTEGLYEIRIKLGNDIFRIFCFFDHGKLIILANGFQKKTQKTPKKEIE
                     RALKIKEEYENENK"
     gene            complement(88068..88583)
                     /locus_tag="PK252_00445"
     CDS             complement(88068..88583)
                     /locus_tag="PK252_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13205.1"
                     /translation="MKAPKITRSELRTGDTLLYEKTIAVGRIEQIFTRSKFNHSSLVE
                     VKEDGILCYEAIFPRVVCRPIEQSIVHTVNVVVKRPRLSFDAAKYVELSKSLVGKRYD
                     VGGLLAQIPHQLWGGWIGNKSMKKVYCSKGNAWVWNQLCQLYPDWYKVSPDELFEDLL
                     DFDTYILELPV"
     gene            complement(88792..89352)
                     /locus_tag="PK252_00450"
     CDS             complement(88792..89352)
                     /locus_tag="PK252_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT13206.1"
                     /translation="MSELFTIIQSRKSVVSFNPEIDIDQSFIDKMFKAASFAPSANNV
                     QPWRYMYGKKGEVTYEKIYNSLTEGNKRWAKDAPLLIASIAQVEYERNGTIVKNKYAW
                     HDTGMANIQLMLEATAQGLVTHPMGGFDAELLKNALQLSQEYEPIIVIALGYAGNNEL
                     LPDDLKERQQKPKMRKEVEEIILKLN"
     gene            89850..90266
                     /gene="ndhC"
                     /locus_tag="PK252_00455"
     CDS             89850..90266
                     /gene="ndhC"
                     /locus_tag="PK252_00455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT13207.1"
                     /translation="MPTIVVFLLYVMGAVFLVAFMLGASHVLGERHEARFRNEPFESG
                     MPLTGDARVRYSAHFYMIAMFFVIFDLDAAFIITYAVAYKELGWPGYIGIAVFIGLLI
                     AVLAYEWKIGALDFGPNGKKIIGATPKNTITEKSLE"
     gene            90341..90979
                     /gene="nuoB"
                     /locus_tag="PK252_00460"
     CDS             90341..90979
                     /gene="nuoB"
                     /locus_tag="PK252_00460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOT13208.1"
                     /translation="MKFSLNPAERVGMGDYPKLSLEQAVQKSVLFANLDDLIGWGRKY
                     SIFPFGFGLSCCFVEFATAWTSRFDIARFGAEVLRGSPREADLMIVAGTPFIKMVPTI
                     KRLYDQLMEPKWVISMGSCANSGGMYDIYSVVQGIDKIMPVDLYVPGCPPRPEAFLHG
                     LLLLKESIGKEQRPYSWTVGPQDVYKAQKPSLRDLNNERRMAVDKLKPADEI"
     gene            91004..92737
                     /gene="nuoC"
                     /locus_tag="PK252_00465"
     CDS             91004..92737
                     /gene="nuoC"
                     /locus_tag="PK252_00465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HOT13209.1"
                     /translation="MIIDELKARFGADNIREQKTSDGMPTAWVNASDILRLLDFVKNG
                     ISKPYKMLYDLTVVDERHRQYRDSMPAADFSVVYHVTSYGRNEDLRIKVALHGEYPSL
                     PSITGLWANANWYEREAYDMFGVKFEGHPNLRRILMSQSWKGHPLRKEYPARATEIGP
                     FSMSEDERTAAEEELVFKPEDWGLKRKSEDAEFMFLNLGPDHPGTHGLLRLILQLDGE
                     EIVDIVPDIGYHHRGAEKMAERQTWHKYIPYTDRIDYTAGVLNNMAYLMSVEKLDGIE
                     VPARAQIIRVMTGEMFRIISHLLWYGTFAQDLGQISPVFYTFNDRERVFDIISAITGG
                     RMHANWFRIGGVAQDLPKGWQQMVLDFVKYFPKSIDEWDKVIMSNRIIKGRTQGIGVF
                     NTDEAIEWGITGPSLRATGFGWDLRKQRPYSGYENFKFDVPVGKNGDCYDRARVRIEE
                     MRQSLSIIEQCVKLMPDGPYKSNSPLTTPPTKENTTKDIETLIHHFLNVSWGPVIPAG
                     ESMVQVEGTKGSNGYYLVSDNNTAAYRCRVRTPSFPSIQMVPYIAKGYTIADLLAILG
                     AVDFVLADLDR"
     gene            92853..93317
                     /gene="nuoE"
                     /locus_tag="PK252_00470"
     CDS             92853..93317
                     /gene="nuoE"
                     /locus_tag="PK252_00470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT13210.1"
                     /translation="MLSEKEIHEIEHEIGLYPVKSAACIEALKIVQEHRGWVSDESVK
                     DIAAILEMTPEDVDGVATFYNMIFRKPVGRNIIFVCNSVTCYMMGYESIYQYMSKKLG
                     ITWGQTTSDGRFTLLPNPCLGDCDHAPAMMINKDHYNRIKPEMIDSILEKYQ"
     gene            93336..94610
                     /gene="nuoF"
                     /locus_tag="PK252_00475"
     CDS             93336..94610
                     /gene="nuoF"
                     /locus_tag="PK252_00475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT13211.1"
                     /translation="MTTKPLTQNIKPDGTVCSLEEYLKNGGYEGARKAVKEMEGAAVI
                     QAIKDSNLLGRGGAGFNTGLKWSFVRQDDPNEQRYIIANGDEMEPGTFKDRLLLEGDP
                     HQLIEGMIIAARAINATKGFIFVRWAYKKAHKLIQEALDDAYKAGLLGKNIMGSSFSF
                     DLYLHTSVGRYICGEETALINSLEGKRANPRSKPPFPQVSGLFGKPTIVNNIETYCNL
                     PHIIKYGSIWFKELSKSGEGGTKLYGVSGRVKNPGLWELPLGTTVRELLEVHAGGMLD
                     GYKFRGLLPGGASTDFLVEEHLDTPMDFSSVAKVGSRMGTGTMIVMDDKTCPVAFVHN
                     LIRFFSNESCGWCTPCRDGLPWVEKILTKIEAGEGEEKDIDILKFHTKYLAPGNTFCA
                     LAPGAMEPLQSALKYFGEDFERHVKEKRCFYK"
     gene            94636..96939
                     /gene="nuoG"
                     /locus_tag="PK252_00480"
     CDS             94636..96939
                     /gene="nuoG"
                     /locus_tag="PK252_00480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOT13212.1"
                     /translation="MATIIVDGKQYQAEAGDNLLKACIKLGIDIPYFCYHPALGSVGS
                     CRLCAVKRFRDADDKRGRIVMSCMEPVSEGMIISVNDEEVKNFRASVIEGLMTNHPHD
                     CPVCDEGGECHLQDMTVMTGHNNRRFEFKKRTQKNQNLGPFIHHEMNRCIQCYRCVRY
                     YRDYAGGKDFGVFGSHNHLYFGRFEDGQLESEFSGNLVEVCPTGVFTDKTLKKHFTRK
                     WDLTNAPSLCTHCGVGCNTIVSERYGTVRRVMNRYNHHVNGYFLCDKGRFGYEFVNDP
                     SRITVSSYKGKALDVESALLKATELIRSGKTIGVGSPVASLEANFALQQLVGAENFYA
                     GVSKAQWELVCAVRDIYRSKNVKTLSLAEVEKAEGVLILGENIINTAPRAALSVRQAV
                     KNKPFAGAAALNIADWNNSAVKIAVGTEKGPLYIFASGKNKLEDVASISKDIDTAYVE
                     KIAEAIAASLKGNLNTVSLNEDEKATVKQITDNLLSCKKVAVISGTSSMSKKLLALSA
                     EIASLLNTSSCESGIYLVVPHANSLGLAVLTSNTIEQVADAAENVVLLEANSIEESVI
                     TFSEKAKNVIVLVYKSPEKLNSKALVLASATFAESEGTLVNNEGRMQRYFRVKNTGED
                     VKASWMWLSQLKAMLDNSNYLSFDSLVKKIGESYPELNKIVDVAPGAGYRESAMKIPR
                     QTPRFTGRTSMYANVDLHEQKPQNDENSALAFTMEGSREVHPAQLNAIYWKPGWNSVQ
                     ATNHYLRDLDNILKAQQMGINIWNVEK"
     gene            96955..97902
                     /gene="nuoH"
                     /locus_tag="PK252_00485"
     CDS             96955..97902
                     /gene="nuoH"
                     /locus_tag="PK252_00485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOT13213.1"
                     /translation="MAIGFIIVGVLLLVVTIAAANIYIERRLLSLWQDRRGPNRAGPF
                     GFLQVPADMIKIFLKEDWIPPFADKAVFVLAPTIVMLSILFSFALVPFAPGFQVVDSN
                     IALLFFLANSSLAVYSIVLSGWASNNKYAMIGAMRGTAQMISYEVFMGLSLMGVVMIS
                     GGTFSLREIVEAQRGMWNVVPQFVGFCIFMIAAVAESHRAPFDLPESEGELVAGFHTE
                     YSGIKFGMFFVGEYLGITLISAMIVTLFFGGWLGPAFLPPFFWFMLKTFAFIAFIILL
                     RAALPRPRYDQFLGVGWKILLPLTLLNVLITAGILLARG"
     gene            97923..98438
                     /gene="nuoI"
                     /locus_tag="PK252_00490"
     CDS             97923..98438
                     /gene="nuoI"
                     /locus_tag="PK252_00490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOT13214.1"
                     /translation="MLSILRSIWITFTHAFRKRETRDYPEKAPFLPARYRARIILTRD
                     PEGKERCVACNLCAVACPTDCISLQATEDETGRRYPEFFRINFSRCIFCGYCEDACPT
                     NAIQLTPDFEMAEYYRKNLVYEKENLLISGPGKYHDYNFYKNAGIEIGGKKKGEGLNE
                     EPPIDVRDLMN"
     gene            98456..98986
                     /gene="nuoJ"
                     /locus_tag="PK252_00495"
     CDS             98456..98986
                     /gene="nuoJ"
                     /locus_tag="PK252_00495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT13215.1"
                     /translation="MSILFLISAVVAVFSTAMVITRHNAVHALIYMVISFIAASVMFY
                     TLGAPYAAVLEIIVYAGAIMVFFVFVVMMLNMSVSPEDEKRATGIKSWIFPIILTLVL
                     IADFVYAFIQGTPQTNMVKVIDAKRVGVSMFTTYLLMAELAGILLLVAIVGAYHLGRA
                     PKRNIHRYLQNEQTQK"
     gene            98993..99292
                     /gene="nuoK"
                     /locus_tag="PK252_00500"
     CDS             98993..99292
                     /gene="nuoK"
                     /locus_tag="PK252_00500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT13216.1"
                     /translation="MIPLEWTLLVAGILFALGLTGLLVRRNLLFMLMSLEIMTNAAAL
                     AFIAAGSHWMQPDGQVMFIFILTVAAAEISIALALVLLMFKHNKTVDTSKLDSLE"
     gene            99318..101192
                     /gene="nuoL"
                     /locus_tag="PK252_00505"
     CDS             99318..101192
                     /gene="nuoL"
                     /locus_tag="PK252_00505"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOT13217.1"
                     /translation="MLQFIWLIPLFPLLGFLLLALGGKYYSKKLVAIIGSASVGISAL
                     LTFVIGLPMLQNIENMPSVTNVLYTWIDVGSFSVPFSFKIDALSLVFVFVITFVGFLI
                     HIYSTEYMAQEEGYARFFAYMNLFVASMLILVMADSLLLMYLGWEGVGLCSYLLIGFF
                     YRDPANNYAARKAFIVTRVGDAAMLIGLLMLVFAFNTLDMAQISDLAKNSVSISPAFA
                     TIVALLLLGGAVGKSAQLPLQVWLPDAMAGPSPVSALIHAATMVTAGVYLIARMHVIF
                     MLSPFAMLVVASIGAITLLIAGVSALYQTDLKRVLAYSTISQIGYMFLALGVGAWSAA
                     VFHFMIHGFFKALLFLGAGAVILSLHHQQDMFKMGGLYKKMPVIFWTFLIGSASLAAF
                     PFISAGFFSKDAIIWYALSSPQGGWVFWLAAMIGAIITCLYTFRMIYVTFFGKEKTHV
                     HYTPGIAMTLPLIVLAILSTLAGYIELPHNMGHIQWFSSFVTNYLPGITVSEHFNEWV
                     FQIIAMVLSLGALAIAFKYYNEKYVESFTMTPVKLFFQNGWNFDKLYDTLFVKPFVWL
                     ANANKEDATDKSIDKGAIQVSKLSNRLSVLQNGRVGWYLAGMAIGIILVVSLMLFIKM
                     "
     gene            101218..102741
                     /locus_tag="PK252_00510"
     CDS             101218..102741
                     /locus_tag="PK252_00510"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT13218.1"
                     /translation="MALVGILAVLILGALISLPVRRIHKQLPWYVGIVAVLTAIIMSF
                     SLGNASSGTEKWFARFTSSWIPAIGSNFSLAIDGLSYIMILLTLVMGLVAIGFSERRE
                     NSSSFIFNSLFMLAGVVGIFMATDLFLFFFFWEMMLLPLYFLIISANKEGSDKAAFKF
                     LIYTQGSGLIMLISLLALYFIHGNQTAIYSFAFEDLLNTQMSITTSMLVMSGFLLAFL
                     VKLPVWPLHGWLPSAFKTAPAIAIFSGLLIKTGAYGIMRFAVPLFPEGSLIISPLMMI
                     LGVVTILYASFIAFSQNDIRVISAYSGISHMGFILIGIYAFNDLSWQGVGVQMIASSV
                     GTASLILLGEIIHRRAGTYDIAHLGGIWTSNPNIAGIGTFFAMAALGLPGMANFIAEF
                     LILAGAFQISVLSTVLASIGIVAAAAYSLRIIQKVFIGEEKICISIIDVTLREKAAVA
                     VMMVLLLWIGFYPSMVTSRLEPFITRTLNFSPPETPGTEQGNVDEAQVKYSFQVIKK"
     gene            102753..104150
                     /locus_tag="PK252_00515"
     CDS             102753..104150
                     /locus_tag="PK252_00515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOT13219.1"
                     /translation="MFVALAPMYIIAAAIVVLMLLAAFVRNHKLLAIVSALGFIAAFG
                     TVFSHCSCLSKFAWELFVVDDYSWALTAVTLLSALAINIFSYFYFENFDEQKEEYYVL
                     LHTAVLGTLVMIAAKHFVTFFIGLELLSIPTYVMVAYVRSKKESVEASIKYLATAAAS
                     SAALLFGLALIYAAAGTMDIYTVGRFFYIAQSVPAYTYVGIALFIVAVGFKLALAPFH
                     MWSPAVYQGSAAPVTAFVASVAKLGVIAFVLRLFNESNLLNIGALNISLVVLAVLSMF
                     IGNLFAVGQKNIKRMLAYSSVAHMGYLMVALLAGGMVAKSATGFYITSYFVATLALFG
                     AISVISKNDEPKSIDSLKGLYYRNPIVAVVITIGLLALIGMPLTGGFMAKVFVVKAGA
                     GIQQWFLVWMLIINSIISIYYYTKYINVLFSHTSEEVVKEKVPYLLATILIIIVVLVI
                     WMGVAPQLWLNLFYN"
     gene            104161..104640
                     /gene="rnhA"
                     /locus_tag="PK252_00520"
     CDS             104161..104640
                     /gene="rnhA"
                     /locus_tag="PK252_00520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOT13220.1"
                     /translation="MTQSKPYNITIYTDGASSGNPGPGGYGTILMSGPHEKVLSGGFR
                     LTTNNRMELLAVIVGLEHLKFEGCNVTIYSDSRYVVDAVERGWLFDWEKKGFKDKKNP
                     DLWKRFLLLYRKHKIKFIWIKGHADNPYNQRCDWLAVNASKSDDLMVDHGYEEGLKN"
     gene            104717..105250
                     /locus_tag="PK252_00525"
     CDS             104717..105250
                     /locus_tag="PK252_00525"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT13221.1"
                     /translation="MPKQEVILHYQGIFNFEIIGHLLNNLKDESEARNISISHYKKIL
                     SVMIEALENVFKYCENFEKEPGLFPQYYPFFSLEFKNGGYVVTTANPILKSDIEKLTS
                     HIDKINSLDKEGLRQLFRATLTNGQFSAKGGAGLGFIEMAKTSCEKIEYTFVPINERF
                     SYYTCVVNISQNALNVE"
     gene            105250..105651
                     /locus_tag="PK252_00530"
     CDS             105250..105651
                     /locus_tag="PK252_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT13222.1"
                     /translation="MNNFYLAETEFTPEISFNIEKRKLAFKGVSRPEDVVKFYQPAVN
                     WLREFDENVLSHSSAKLNIPSIHVEFRLTYFNSASSKMLLQLLEIIKKVQDKGIDIII
                     DWYYDANDEAMYDDGADLSEAVNLPFNFHTI"
     gene            complement(105723..106775)
                     /gene="rfbB"
                     /locus_tag="PK252_00535"
     CDS             complement(105723..106775)
                     /gene="rfbB"
                     /locus_tag="PK252_00535"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT13223.1"
                     /translation="MKRTIIITGGAGFIGSHVVRLFVTKYPDYKIINLDKLTYAGNLE
                     NLRDIDQLPNYTFEKADITDAKRIGEIFAKYQPDGVIHLAAESHVDRSITNPLEFIQT
                     NVVGTLTLLNAARDAWKGNMEGKKFYHISTDEVYGSLGETGMFTEETPYDPRSPYSAS
                     KASSDHLVRAYYHTYKLPINISNCSNNYGPNHFPEKLIPLSINNIKNNKPIPIYGKGE
                     NVRDWLYVVDHARAIDVIFHKGVIGETYNIGGHNEWTNIKLIKLLCEIMDKKLGRAPG
                     TSAKLITYVTDRAGHDLRYAIDATKIKRELGWEPSLQFEEGLEKTVDWYLANEQWLNN
                     VVSGDYEKYYAKQYNR"
     gene            complement(106849..107649)
                     /gene="rfbA"
                     /locus_tag="PK252_00540"
     CDS             complement(106849..107649)
                     /gene="rfbA"
                     /locus_tag="PK252_00540"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT13224.1"
                     /translation="MSKQMMPVYDKPMIYYPLSTLMLAGIREVLIISTPRDLPMFEQL
                     LGSGEDIGMKFTYKIQEVPNGLAQAFVLGADHVGNDSSCLILGDNIFYGAGFSAMLHE
                     ASALKNGAIVFGYEVKDPRAYGVVEFDKTGKAISLEEKPQVPKSNFAVPGLYFYDNKV
                     VEKAKALKPSARGEYEITDLNRIYLEEGNLKVQKFGRGFAWLDTGNADSMLEAAGFVE
                     AIQKRQGLYVACLEEIAFRNGWIDIKQLKKLGESMSKTEYGQYLLSLE"
BASE COUNT        30479 a        20076 c        22120 g        34876 t
ORIGIN      
        1 ataaaagtaa ccccgttgcc gcctttgcca cagccaaagc atttgtaaat acctttgctt
       61 gccgatacag taaaagatgg ggttttctcg ttatgaaacg ggcaatggcc aagataatta
      121 gcgcctctgc gtttgagtga cacaaaatcg cccactacat caataatttg aatggcatcg
      181 tatattttat ctatggttgc ctgatctatc atcggataaa tttgaaatgg ctaaattaat
      241 gcatttaaag gtttttgcgg acgtaaaaaa tgggcatagt gtaagtattt gacaatgtgt
      301 ttttaagcta attattaaca atggttaaaa ttttattaaa tacaataatt ttcggcaaga
      361 cttttgaaga ataatgatta cttttcacca caattttaaa gtaagacatt gttctttaaa
      421 gaatttaaat agtttgttat gggagggaat ttagtagtaa taggaagtac aaacgttgat
      481 ttaaccatgc gtcttgacca tttgccaagt agaggcgaaa ctgttggcaa tggcgatttt
      541 taccaggcat ttggcggaaa aggggctatt caggctattg cagcagcccg ttgtggcgga
      601 aaggttaagt ttatttcctg tgtgggcgat gacgattttg ggcgccttgc tattgaaagc
      661 cttaaaataa atgggattaa tgttgacaac gtttttgccg aaaacggtct aaatactggt
      721 acagccctta tttttactga tgttcagggg aacaattgcg ttggtgtttc tcccggggcc
      781 aatgaatcat tacttcccga aaaaattgca agtgcaagtg cggcttttga aaatgtcgaa
      841 atggtattgc tccaatgcga agtgccttat gattctatca agtacgttat tgacgcttct
      901 acaaaataca acaaaaagct cattttgaac ctggctcctg cccgtaatat tgatgtttct
      961 tatttgtcaa aagctcatgt gcttgtggtt aatgagcccg aagcagaatt cctgagcggg
     1021 ttcaaggtaa ataaagactc agatatagag catgtagctg aactgctttt ggctaaaggc
     1081 tctgaacata ttatcattac gcttggcgct cgcggtgctt attatgctac ctccggacaa
     1141 aacttttttg ttgaaagctt ctcggttgaa gcaaaagatt ctaccggtgc aggcgatatc
     1201 ttttgtggag ccttggctgt tgcccttacc gagaaaaagt cttttaaaga agcgctaaca
     1261 tttgcttctg ctgccgctgc tataagcgtt acaaggttag gggcgttccc ttctgcacct
     1321 cgcagggctg agattgaaga gctcgcagcc aagaggtaag ccacgaaaat ttttatagaa
     1381 ttttcgttcg tgatagtttt aattgcattc aacctgtaat tttctttttt cgtcagaatt
     1441 ttgctgtgct tttttcagtg ctaacttatg tttgcatgag aaaattaatc atcgtggaat
     1501 taatgacgat aaagtacttt ttaggcatgt cattgataat gcttaatatt tcgtgcaata
     1561 aataccaggt atcagaccat tgtgatggcc ggaaattttt taacccggag aaagggcact
     1621 cgtttggcga ccaaataaaa tggatgtacg aaataaaaac cattgattgg ccccgttgga
     1681 tagaagatat accgcagcct gctcctcctc ctgctgtgtc agggggcagg gtaagggtga
     1741 cctatattaa ccaagccact atgttgattc aagtaggggg cataaacatt cttactgacc
     1801 cgattttctc ccaaaaagct gggcctgtat catggttagg cacaaaaagg gttagaaagc
     1861 ccgggattga catagatagt ttgcctaaaa tagacgtaat tcttatcagt cataatcatt
     1921 acgaccatct tgatgccaaa tcgttgaaga aactggcatc aaaatatcag gctactgtat
     1981 ttaccgggct tggaaataaa aaaatattgc agtcattcgg ttttaaaaag gttatagaaa
     2041 tggactggtg ggataaacat gcaatcaatg aaggtacagg atttgttacc tttgttcctg
     2101 cccgtcactc ctcaggccga ggactatttg ataaagataa aacactgtgg ggaggctttg
     2161 taataagcac tgcaaatgga gatatttatt ttgctggaga tacagggttt ggagaatttg
     2221 ctaaccagat acaatctagg tttaaaaaat tttcattagc catattgcct attggcagct
     2281 atgagaaacg ttggtttatg aaaagccagc acatgaaccc ggatgacgcg gtgcgcctgc
     2341 atttgatgtt gaatgtagaa caaagcatag gcatgcacta tgccactttt aatgagcacc
     2401 ccgaacaagc tattgatgct catgagaagg atttgagaga agccctgctt atccataatg
     2461 ttgatgcttc aaaatttaag gttcttggtt ttggtgaagg gtacgagatg gatttataga
     2521 gagttctcct tggttgctac gcacgatttg taaaacaggt cgtgcgtttt gcttttatac
     2581 aatatatatc aatgtcgttt agttaagaac agacccttcg actccgttca gggtgacggt
     2641 caccctgaac ggagtcgaag ggtgcttggt aattgaattg ttgaaagtat agccttaact
     2701 aaacgacatt gcaatatata taatttttat tcctgcttcg aaaactttca ttcccccttt
     2761 atttttctgc actcagggta tctggtaagc actcaggcat tggacttcta tagttttggc
     2821 caaaaatcaa aaaacaatga gaaaggtaaa aactatttta acaaaagctt tattatttgg
     2881 ctttttaatg acaggctccc ctttagctgc acagctttat caggctatca gggggacaat
     2941 aacagacgtt gacagcaagg ccccaataac cggggctgca attgttattc aggatgtgga
     3001 gccagcaatg gcggctattt ctgacaaaga tggcaatttt gttgtgccta aaataccagt
     3061 tgggcgccat gaggtgaagg tttcgttcgt aggttatgat gcaaaagtca tttctgagct
     3121 tttggtttca actggtaaag agctggtttt gaatatagaa ctgaaagagt caacaaccaa
     3181 tattgaagag gtcgttgtaa aggctcaagg gaaagataaa cccgtaaatg ctatgacaac
     3241 cgtaagctca agggctttta ctgtggaaga aacccgtcga tatgctggag gattggatga
     3301 tccaggtcgg ttggcctctg tttttgcagg tgtttcggat gggaatatcg agtctaacgg
     3361 aattattgtc cgtggcaatt cgcctacagg agccatatac cgaattgagg gagtggaggt
     3421 gatgaacccc aaccattttg ccggcgaaga cctgctggga ggcggctttg tgtcactgat
     3481 tagcaaccag gtactcgaca attctgattt tcttaccggg gcttttccgg ccgaatatgg
     3541 caatgccttg tcagcggtgt ttgacatgaa cttgcgtaag gggaataatg aaaattacga
     3601 gcatacattc caggcaggtg ttatggggct tgattttgca tctgaagggc ctatagctga
     3661 aaaagggaaa gcctcatatt tgttcaacta tagatattct acctttggat tggtgcagaa
     3721 tttcttacct ccaggcgaag gattgcctgt atatcaggat ttatgcttca atatcaatat
     3781 tcctgtaaaa caaagcagca tttcagtatg gggaattggg ggaattgatg gttattcttt
     3841 caaaaaagaa attgatgata agtaccagac cgctattggc ggcataaaaa acaagctggt
     3901 gttgaacaat ggcctatata tcaatacttc gttgacctat aatgtttttg ttaagaaaaa
     3961 caggtggttt gaagaatatc agtcgcagta ctaccccaaa agcaagattg ataatatcga
     4021 aggcagctat accttttcaa cctttttgaa caaaaagttc aacagatgga ttaccaaccg
     4081 ttcgggcttt agcgtcaaaa acatgttttt tgacatcgac aataaatact ctgaaagggc
     4141 tccacagcca cttatgcaga taaaccgtgg tgatggcgac tgcaatcttg cccaggcttt
     4201 ttcgcagatg aagattacct taaatgagca tctgatgtta aataccggct tccacgtgca
     4261 gtatttgtcg ttaagcaatc aatactcctt agaaccacgt tttggggcaa ggttttcaat
     4321 caatggacaa catgccatta gcgctgctta tggacttcac agccagatgg cattgctgaa
     4381 cgtttatttt attcaaaaaa caagcaacac aggtattgaa aacccaaata aaaagctggg
     4441 ttttactaag gccaaccact ttgtgctggc ctacgactgg aatataaaca agaacctcag
     4501 ggtaaaaatt gagccatact accagtattt atttgatgtt ccggttgtta aagatagttc
     4561 gttttcaatt cttaaccttg atttaggaaa tgattttcag gaaacacttg taaacaaagg
     4621 caaaggcaaa aactttggaa ttgatttcac gctcgaacgc tttttgaaca atggttttta
     4681 ttatctggct acggcctcag tatttgaatc aacctatgca ggaggcgata acatttggcg
     4741 aaataccagg tataacaaga acttcatggc caatatcctt ggcggaaaag aatggagcat
     4801 tgacaaaaac aacaaaaaca aggtgtttgg tattaacgga aggctttact tcaaaggagg
     4861 cgaccgcatt agccctatta acgatgcatt gtcagcccag gaagaaaaag tgatgtatga
     4921 aagccaacag gcatttgaaa aaaaataccc cgcaacctat cgttttgatg tcagcctcag
     4981 ttacagggtc aacaagccca gatacagcag tatatggtcg ttacaaatta tgaaccttct
     5041 gtattctacc attaatacct actatgaata tgacaaggta agccgtacgg taaataaaaa
     5101 agaagagaag tttgttttgc cttcgataag ctggaaagta gagttttaaa ctagttaatc
     5161 aatgtcaatg atgatgaaag ttttaaaaat tatattaata attatccttt tgttttctat
     5221 gaaagatgtt ttaataaatg ggcagggaaa agacaacagc tttagcaaac cctctagaat
     5281 aggggtattt tggcattcgt tcttctcccg ggtattgatg gggccatttt accagtcata
     5341 ttttaatcaa cttcctttga aaggaaatga aaaagtactt gattttggtt gtggatgggg
     5401 cgctgaagct cgaattcttg ctaagaaact ttgcaaaggt ggtcggttag cttgtcttga
     5461 catctcgcct caatggatag ctgaaacaaa agaggtttta aaaaagtaca ataatgtgga
     5521 ctattatgta ggcgatattg ttgtgcttaa catcccggag agttcatttg atgcggtatg
     5581 tattcatatt gtcttgcatg atatagatgt aaatctcagg ccagacatag taaaggctat
     5641 tgcccgcata attaaaccgg gagggcactt gtatatacgc gaaccgctta tggccgaaag
     5701 gcagatttct gccagtagta ttgaaagcct catgaataaa gccgggttac ttgaaaggtc
     5761 tgtaaaattt aagcgttcgt tctggtatgg cgagattgtc gaaatggttt ttgaaaaaaa
     5821 aggagaatag ccgtaaattg cagtattata atttgtaaga aaatgggctt gtttaaaaaa
     5881 tatgcttttc tgttaacttt attgtgcggt tttgctgttc atttatttgc aagtgcagat
     5941 tttataagtg aagggaaagt atatttcact attcaaaaag caggttcgtt agttacggaa
     6001 gacaactact taaaatggcc tgttttcaag gattttgact gggacaattt ggcagcaggc
     6061 agccaattgg tattagctgt agattcaaag tctgtattag ctgccgaacc tgcaatatgg
     6121 atcgatgctt atcttctgga atatactatt taccagggag attctgtatt tttttcatta
     6181 accggaaatg atagacatag ccagattgta cctgtggtga gaaagctttc tggtttagtt
     6241 tcaaagcctg tttacattgt tattcccaat actgcaaaga atattttcaa caggatatat
     6301 tctatcaaaa ttggtaatta tgaagatttg aaggccgaag cagaggctta tccaacaaag
     6361 ttctttgctc atcagctatt tttgattgcc attgtatcta tcatcatatt ttcagcgata
     6421 ttgacattgt tgattttttt tatccgttgg catgacaaaa attatgcatt gttatactat
     6481 gcaatagctg ttacaatggc tgctttaagc aatattgacc ttaaagtcct tcattttatc
     6541 gtacattttc cattttgggc acttaactta atcattcttg ttggtcgttt tggcgaagga
     6601 atattgtttt tcctttttat caaatctgtt tttgaattga aacaacggct gataaatatt
     6661 gtgttgttta ttatcctttt atactctggc atttcagtaa ttgcgtatta tatatgcccc
     6721 aatcctgttt atttttctta tttaagtcac gggtatggcc tacttatttt gggcatctta
     6781 tttttatata tctattatat ccgccagtca aatgtttttt tgaagatgcc gttgaaaagc
     6841 ctgctattgt tttttgtatc tgttttttta gctaccatct tgttttctct tttatcgctg
     6901 ttttttatgt atggttcaca tcatgaagtt cctgtgtata ttggagtttt agcgttatcg
     6961 ttagctatgt tggttttgat tattggcaac tacctcagaa aattgaagga aaacaacgat
     7021 tatgttaccg aagcggttaa tcaaaaaaat gaattgctta agcttcagaa agctaatgcc
     7081 gatgctcaat ttgaaacgct gaaaagccaa ttgaaccctc attttttatt caacagccta
     7141 agtacattgt cttcattagt ggcatcagct aacgagccgg gcaaagcaaa gatgtttatt
     7201 gaggaatttt ctcatatatt tcgttatata cttgatatta ggtcaaaaga tgttgtttcc
     7261 cttgccgaag agcttgcctt tgttaagtca tatctttatt tgcaggaggt acgctttggc
     7321 tttggcttga aagttaaata tgacattgat aaaacttata taaattgtgt tgttcctccg
     7381 cttgcagttc agatacctgc tgagaatgct ttgaaacata atgttgtatc taaggaacgg
     7441 cctttattac tggagatatt tactgaaaac aattttttgg tcataaaaaa caacttgcag
     7501 aagaaacagg ttaccgaagt tgaatcaacc cgcatgggta tcgccaattt gaaagagcga
     7561 tattcgcttt tgtccggtct ggcgctcgaa ataattgaaa caaacgaaca ttttattgta
     7621 aaaataccat tattggaaga tgagtatcat taaaacgctt attgtagaag acgaagaatt
     7681 gcttgcttca agcctcgaac agactttggc tcaaatagaa ccttctgttc aagtagttgc
     7741 aaaacttgaa tctgttgtat ctactgtttc ttggcttaaa aagaatacag ttgaccttat
     7801 ttttttggac attcagttat cagatggctt gagctttgat atttttgagc aagtcaatgt
     7861 tgaaacacca gtaatatttc ttacttcata taaccattat gctgtccgtg ctttcaagct
     7921 caatagtatt gattacctgc tcaagccttt aaatacagaa gaactgaaaa atgctttaaa
     7981 caagtataag aaacaggctt tgaagagttt tgacattcaa gagtttagtc gggttatagg
     8041 ggctttaaaa gataaaactg ctgaatacaa aaaacgcttt gttgtttacg ttggagacaa
     8101 aatcaaatac ctggatgccg agaatattgc ctatttcttt atcattgaag gcgaatcgtt
     8161 catgcgggga tttgatggta acacgtattc gtgcgactat tcgctggata agctgcaacc
     8221 tgagcttgac ccggccatat tttttcgtgt aaacaggcaa tacatcatca acattaaggc
     8281 tattaagcaa atgtttaccg tgtcaaaaag caccataaag cttgatttaa cgcctgttcc
     8341 tgatggcgat gtctttgtta gcctgagccg taatagtgaa tttaaaaaat ggctcgacca
     8401 ataaatatca gcttttcttt agccatcttg gtactgcgtc aataaccaca attattaccg
     8461 aaatcagaat taccaaagtc aacaaactgt ttactattcc ctggccttga gtctgggagt
     8521 tgaatatttg cgggataaac aggtttttaa gatttaaaat accagctatt attgttgtta
     8581 cacctacaaa cgccattgga acaatagtaa taacaatata tttctttttt ccgatattga
     8641 ttaaaaacga agttgcaata accaatgcca aagtggccaa cagttggttt gcagtcccaa
     8701 acatgggcca aatggtcgaa acagagcctg tatatatgaa gtaagcccag caaaacacta
     8761 ctataccgct tgccagcagg ttcgatggca ggtgatttac tcgtgcaaaa ggcttgtaga
     8821 gctttccacc tgtttcttgt atgataaaac gggcaatacg tgtaccagca tcaatggttg
     8881 taaggataaa taaggcttcg aacataatgg caaagtggta ccaatacgac atcaggcttt
     8941 ttaagcccgg tattgatgaa aatatctgcg ccatccctac ggccaatgaa actgcgcctc
     9001 ctgtgcggcc agctatgctc tcgcccacag cagcagagag tgaaggtaag tcgctttgtg
     9061 taaagcccat ggcatggagc tggggcaaaa tagcttgcag cttttcggcc gggacgttga
     9121 tcataaaata atcaagcggc aataagcttg aagctgcaat taacgccaat atactgacaa
     9181 cgccttctgc aagcattgac cctacagcaa taggcctgat atgcttttcg ttcatcagca
     9241 ttttaggggt agtacctgag cttaccaatg catgaaaccc tgagatagcc ccacaggcaa
     9301 tggtaataaa caggtaagga tatatattgc ctttgataat tgggcctccg ccatgaataa
     9361 aatctgtaaa tgccggcatt tttaaggttg gcattacaat gatgatgcca agtgccagca
     9421 tagctactac gcccaacttc atgatagagc ttaaatagtc acgaggcgaa agcaacaacc
     9481 atactggcaa tacagaagct ataaaaccat aaatggccaa tgaaatagtg agctgatgat
     9541 gcgtgaatgt gaaatatggt gctaaggacg atccgggaat atttcttcca aaaactacag
     9601 cgaaaaacag taggattact ccaattaatg tggcttcttt agtttttcct ttacggaaaa
     9661 caaacatcca ccatcccatt agaagggcaa taggaatagt agccgcaatg gtaaaagtac
     9721 cccatgagct ttctgaaagg gcattgacaa ctaccagccc cagaccggca agcgctatga
     9781 taatgataat caggacagcc aatgatgcta tcgagccgct tagcttactt acttcttttc
     9841 tggcaatttc ggcaagcgat tcgccattat gcctgaccga agctgttaat attaccaagt
     9901 catgaacagc cccggccaaa acagccccta ctaaaatcca tataaacccc gggaaataac
     9961 caaattgtac tgccaatacc ggaccaacca aaggccctgc acctgcaata gcggcaaaat
    10021 ggtgtccaaa caatacccat ttgctggttg gcacaaagtt ttgtccatcg tacctgcttt
    10081 ttgagggtac ctgattgtgt tcattaatag tcaaaacttt tgctgtaata aaaccaaagt
    10141 aaaaacggta tgccagtgca aatgcagcta acacaccaat aatcagtaac atggcattca
    10201 taaatactgc gtttttgtaa aagaaaaagc aaacgtatta tttttttctt tttgattgca
    10261 ctttatcgtg tgctatttag tagttttatt tttgtaaaaa aattgaaaat tatggaaagg
    10321 ctttttaaca aagaaaaata tatacagcgt cgtaagcaac taaaggaaaa agtaggcttt
    10381 ggggttatct ttatgcccgg aaactttcat ttatcaatga actatccggc caatccgtta
    10441 cgtttcaggc aagatagcaa cttcctgtat ttctttgggc ttgattcgcc cgagttagcc
    10501 ggggttattg atgttgacaa tgatgaagaa ctgttgtttg gccccgaatt ttctgttgat
    10561 gacctgatat ggaccggaca agtagagatg tttgaccagc gggcttttaa agttggtgtt
    10621 tcaaaggttt tttcatactc aaaacttgaa agatatatta aaaaaacggt tgaagaaaaa
    10681 cgtaagattc attttttgcc gccatatcag ggagaaaaga agatagtatt gtctgaaatg
    10741 cttggttttc cggttgcaaa attgaaggaa caagcttcag atgttttgat aaaagctgtt
    10801 gtagcaatac gtagtaaaaa agatgccgat gaagtggatg aaatagagta tgctttgaat
    10861 aatattacgg ctgaaatata tagacaatgt atgtctgtta ttaggccagg cattaaagaa
    10921 tccgagtttg cttctattgc tgaaggctat gcactcaaac acaattgtcg tttggcttat
    10981 cctgctattt gcactgttaa tggtcagtat ttgcataacg aatcgtatga caacatttta
    11041 aaagacgggc aattactatt gattgatgcc ggaagcgaaa gcccaatgca ctatgcatcg
    11101 gatataaccc gcactattcc tgtaagtaag aagtttaata cacaacaaaa agaaatatac
    11161 ctgttggtac ttgacatgca acaaaaagca ttgtcattaa tgaaaccggg tgtgccatat
    11221 tcttttgttc acaaaaacgc ttgtgaagtg cttgttaaag gaatgataca gcttggtttg
    11281 atgaaaggga atgttgctga ggttgttgag gctggggccc atgccctgtt tttccctcat
    11341 gggcttgggc atttattggg gctggatgtc cacgatatgg aagatttagg tgaggccaat
    11401 accggttatg atgaaaccta taaaagaagt cagcagtttg gcacacgctt tctcaggtat
    11461 ggcaaggagc ttcacgaagg tattgttatt actgttgagc ctggcattta tttcattccg
    11521 gagcttattg accaatggaa aagcgaaagc cgttattctc aatttatcaa ttatgcggct
    11581 ttagaaaagt ataaaaattt tggaggcatt agaatagaag ataatgtact gattacaaat
    11641 gataattaca aaattttagg caaccttatc ccaaaatctg tttttgatgt tgaagcagcc
    11701 tgtaatttat aattacaagc ctatccatcg tttcaacctt tctttgaaag tgaccggagt
    11761 gtcatcgtca tcactgtaat agcctttgct ataactatag tcgctgtagc tgtacccata
    11821 atggtactta tagccatatt ttccgccata gtgcgaggta taaccatagt aacccggtat
    11881 tttaacatca ttaatcaaca catttaagtg tggcaactga tcattttcag ccaattcatt
    11941 gattaagtgc actacgtctt ttgtcgagta gttttgacga acaacgaaaa gagagcagct
    12001 ggcatatttt gatataagca aggcatctgt aacaacagcc acaggaggcg tatcgaaaat
    12061 gatataatca aaatcttctt ttaccttttt aatcagcttc tccatattct ctgtttcgag
    12121 cagttcggcc gggttaggtg gtacaggccc ggccggggca aaaaacaggt tgttgatatt
    12181 agtagggaca ataatctctt catattgggc ctggttactt aggtaactgc ttatgcctgt
    12241 tttgttgggg atattcagat acctgtgtat tttaggcttt cttaagtcaa aagccatcaa
    12301 taaaactttt ttattagcca tagaaagcac tatccctaag ttaactgcgc aaaaagtttt
    12361 tccttcacca ctgatggtag aggttactaa aatagatttg tcgtcttttt cgcgcaggat
    12421 aaattgcaaa ttagtccgca ggcccctaaa tgactcagcc agcgatgact ttggatattc
    12481 aaataccgga gtctcggtat ttttattgtt atgccctaat gaaacaataa tagagctctt
    12541 tcttaaccgc gaaaggtctt ttatatctac tatttgatta ttgagaaatt ccatcaggaa
    12601 aataataata aacggaatag ctaaccccaa taaaatagcc gttgaataaa cgctcgaacg
    12661 ctttggtttg attaaaacag cgtttgttga cagcgcttca tctattactt tattatcggc
    12721 tttatttgag gcccgcgtta tacctgcttc ggctcttttt tgcaataaga aagtaaatat
    12781 actattgtta atatcatatt cacgttttat cgaaaggtat tccctttcaa tcggaggtaa
    12841 attatataat ttttcttctt ctttctgaat ggcttcttca caaatcctgg tatcgtacaa
    12901 tgtagtattt tctaattcgt ttaagctttc taccagtgtt ttgcggacat tatcaatttt
    12961 taggtttaaa attttgaagg ccgggcttgt ctctgaaaca gaaagtttta attctctctt
    13021 ttggttatag aggtcaatga gttgggtaat ggatttattt aaaagatcat cgtttaggcc
    13081 aaaagtaccg ggagccataa catccgaaaa atcagccttt ttatttatat agtctctgat
    13141 gtagctacaa tatttaagct ttatgccatt ttcatatttt tgcttttgca agcctatcaa
    13201 tcgttctgaa attatttttt cttcgtcgag gatgtcaaaa acttttcggt ttgtacgaaa
    13261 atcctgcaat ctcaattcat tcttttttaa cgagtctcca actacattta gctggttatc
    13321 tataaacctg atagcatttt ctgcagcttg gtttttttcg ttcaggttat ttcgcaaata
    13381 aatttcgctg actttattta caaactttgc ttctttgtct gggattgggc ctgatgatga
    13441 gagtgtaatt atcgaacctt gtttttcatc tccggtaaca cttagctttc caatatattc
    13501 gcgggtaaga ttatttaaat cgttaatcac aaaagaatat tggagttttc tgtcttcccc
    13561 ggcagcctgt gggttgaaat cttttatttt aattatccaa aaagagaagt cttcattctc
    13621 aaatttattt ccaaacctga tacgtttttt agttttgttt tgctctccgt actctaataa
    13681 aatttcttct gagtttaaaa aagtcaggct taccggaaca tatttcaggt ttgttttgga
    13741 tgtgtctaat acgatctgaa aaaactggtt aaatactttt ctttctttta ttcggccaac
    13801 aacattatag gtaatttcaa aatttagttc tatcaatgat tgattaataa cagaataaga
    13861 ttttattttg gcaatctcat taaacatgtt gcgtttagta aggtttcgcc ccattaccgg
    13921 gaaaagcgat tcaaagcctt tttgttcttc attgatgata atagtggagc ttactttata
    13981 ttgaggttcg gtatatctga ttaccaaata agctattgat aacgctatcg ttaaagaaat
    14041 aaggaataac ggccagttta ttatggtttt aaataaatac cttttaatgt taggggcctc
    14101 atcagcaggc tgtatgttgt tttctatttc gttcatccga aaccttattt ttgtactaaa
    14161 ttataaacca gcaaacttaa tgtaacaact gaagatacgg aggtaataaa caaagccagc
    14221 tcatttaatg ttaacctgac agcagtaatt ttggctggtt caacgtaaat tatatcatta
    14281 ggctgaacgt aaaaaagagg gctactgatc aggtttttgt ctgttacatc aaccctgaat
    14341 gtttttatcc cttcaggggt tgtacgcata attaacacat tgtgtttatt ccctgatttg
    14401 aggatatctc cgctcatagc aaatacttca aacaaattgg ccctttcggc cataatattt
    14461 ttttgtcctg aaccaatttc gcctaatact gttattttaa aacttagcaa ccttactact
    14521 acttcagcgt cagaaactat ctcccgggct ttgttttgca actcttgttc tatttctttc
    14581 attgtcaggc cggctacctt aatatcccct aacactgcaa tatttatata gccgcccaaa
    14641 tcaaccggat atcctataag gtaataattt gaagtgttat aataataagt attttctgat
    14701 gttgaagagg ttttattaaa tatttcagca gctgtggggt tgatagaact tcttattttt
    14761 atatataaaa tatctgaggc ttgaacttta taagtagtat aagttgtctt tattagggag
    14821 tcagatgttg tttttcctaa atctctaaca tatacaatgt ctttgtaacg agtacacgac
    14881 gcacctatta ctaatactaa tagaaagaaa aaaaatcttt tgtgcattta aatattgtta
    14941 ggtttttagc ttcttctttt attaattagt cggtaaattt acaatttaat ttttaaacat
    15001 gaaactcccc atgctttaga aggctgccat gagcaggtct atatcgctgg atggaatgtc
    15061 gagttgtctg gcaatatgcc tgttagtaag gatgccgtta aaaatatata ctccgtgcct
    15121 aagcccaata tctgttttta attgctcgtt tatacctccg ctttgggcta tttggttaag
    15181 aataggtaaa aagatattgc tcaaagcaat ggatgaagtc cttgaaaccc ttgcaggaag
    15241 gttgggtata gcataatgga ttactccaaa ttttgtataa acagggtctg aaaggcttct
    15301 gtactctgag ctttcaatac atcctccata cataaggcta aggtcaacaa tgactgaacc
    15361 ctttttcatt ccgcgtacca tttcgtctgt aacgtagaac atgatgttat tctcaaatgt
    15421 ttcgcgggct ccaataagta catcggccga ttttaaagcc tttgcaagta cctggggatg
    15481 aaatacagag gtttgtaaag gctttcccaa tatattttgc agttgtatca gcctttgtat
    15541 ggagctgtca aaaactttca ccacagcgcc taagccatgt gcagcccgaa tggcatattc
    15601 ggctgctgtg ccagccccta ctactacaac ttctgtgggg gtaatacctg caataccgcc
    15661 caaaagcacg cccttgcccc cgcgggcgtt acttaaatat tcggcagcaa taaggataga
    15721 tgtagaacct gagatggcat tcattgaacg aactacaggg taatatccat cagagtcctt
    15781 gatattttca aaagcaaagg ctgtaacttt tttggccatc aggcttttta tatattcagc
    15841 atcataatgg gtgatcccaa gagatgagaa aagtatttgt tgtcccctga gggcatgaat
    15901 ttcttcctga gtaggggggg caatttttag cacaatatcg gcctgaaaaa ctgaatttcg
    15961 gtcttttatc aataatgcgc ctttttcgct ataatcacga tccgaataat tggccgaatg
    16021 tcctgctgac gattcaagta ttatctcgtg cccttgcgat gcaagaattt caacagcttc
    16081 gggggtaagt ggtacgcgcc cttctacctc atggttttct tttgggatgc ctatgaccag
    16141 ttttttatat ttcttttcaa cttcaagcat ttcttccatc ggaagcagtc cgcttttgct
    16201 aaaaactagt ctgtttgcag cctgagaacc catagtattt tgtttatttt ttgtaaaatt
    16261 acatttttat taagaaaaat gtaatttgct gtcttttttt tcaataccgc gtttgattct
    16321 caatgctttc gatatgtgca aaaaagtgtt cgaaaatttt tgcttaaaaa tttttcttgt
    16381 tttttttctg agtatttgca gaaataaaaa agattattac ttttgcacca ccattaatac
    16441 taacaacaat ggtgtaagtg gtaaaattta tgcgggaata gctcagttgg tagagcacaa
    16501 ccttgccaag gttggggtcg cgggtccgag tcccgtttcc cgctcagaaa tcccgattat
    16561 cgggattttt tttcataaag gctgtgctaa ggcttagtct tttaagttca tttaaaggtt
    16621 gcccgaatgg tggaataggt agacacgcag gacttaaaat cctgtgggca gaaatgtccg
    16681 tgccggttcg attccggctt cgggtacgta caagcccttg taacaaaagt tgcaagggct
    16741 tttttattca gagatagcca ttcattaggc tcgacagtgt cttttattcc tgttggtttt
    16801 taacttaaag ggttgtatgt gaattagaaa tgtgcagata ttcaaaacct gcgccataaa
    16861 gtgcctattg ggttaattat tcttaatgcg tatataaaaa cgactattgc tgcccaattt
    16921 caaaaaccgg ttataaattt tcccaaatag cgtttgtgca attatatctt tgcggctata
    16981 agtataataa ctcaaaattt caaaaccatt tttttctaaa aaccatttca tgctttttac
    17041 agttggttca tataaatggt ctggtgtttt acttttttcg tgcttccatt ggtgattctt
    17101 caatttcaat tttaccaaca gaagttttat tttacccgtt aatgagtatt tgtttgggat
    17161 gtttataaca gctactccgt cttccgataa taagtctttg atttttaaca accagttttg
    17221 tgggtccggg atgtgctcaa tgacatgact aagatgaata acatgagaat ttttcatcaa
    17281 aagttaatga agtaaaatta tcacgaaaaa ctttaagatt tagtttattc tcaacaaaag
    17341 aggccatttt ctctgatact tccacaccat aacagtgctt gaaatatttt tttgctgaaa
    17401 atagataatc acccatacaa gccccaacgt ctaaaacaga attgggtgat tttataaaac
    17461 ttaaggtttc tgaaatttct ttatcccagt tctttgtaag cctgtctgaa tactcatagg
    17521 tgatatggtt gaaatcatta tattttgcat aagcatcttc taaaaatttt ccatcatatt
    17581 ttggccttgg ggaaaaataa acaacaccac aaattttaca tttgagatta ttgtattgta
    17641 attcattacc aaatctttca tatacaaaac tattatttga accacaaaat ggacaattca
    17701 cgctttccca tgcgtgaggt acataatttt tatttttcat cttttgataa aattatttat
    17761 agtttctaat gcacaaatat ataataacct taaataaaat cgcagactcg cttaatgaaa
    17821 agaagaaaat ataagcaatt tcgctaacgt tagcgagtgg tctcatttat tattctccaa
    17881 aattgcctta ttcatgattt gtgtatttcg tcaactaagc ttacaagcgt tgttgagggc
    17941 tgttgtagag ctggataatt tatcggaagt agctacaaca ttaagtcata gttgtttttt
    18001 cgtacctgct ggtatatttt tctatcgaag tagtaaagtt gggcaggttt gtgcagcacg
    18061 ctttgttgtt tctcgttcag aggcactatg aatggcattt ttaaaagctt acgcctgaaa
    18121 ttgcgtttgt caaaattacg gccaaggatg gtttcgtaaa ttttttgcat caggccaaga
    18181 gaaaattttt caggcaaaag ctcaaatacc aaagggttgt attttacgcc atcacgcaga
    18241 aaagctaagg cttcatccag tatgaaatgg tggtcataag ccatttcttt aggaaggtcg
    18301 ttaattgggt accaacccgg gttatacatt tgatggtgtt ctttctggtg ctgaataggc
    18361 cactcgtact tgttggcatc tacaagcgaa taaaacacta tagacactac acgttggtat
    18421 ggatcttgcc cgatggttct taaccaaagc acatccctgt cttgacggtt aagcctgtca
    18481 agtgccccaa aggtcctgaa ttggtgcagt tcaatattgt caatacctgt aaattcgaag
    18541 agcacccttt tagcagccga attaatatct tcgtcaaaat tgacatggct ccccggcagg
    18601 attttgtcag tgaatattat tttccctgaa gcaggctcag tcataatcct atcgagcata
    18661 aggacattaa gtttttcgaa atcgtaaccg aaaatgacac aattgactga aacaaaatcc
    18721 tgaaaatcca tttctgtgta taattctttg gtttttgtac aaatataggc cagacggttt
    18781 ttataaacaa cttttattcg tttattaaag aatatttttg gtcacgcagt tgaggtgtaa
    18841 aacgtgcatc aatgatggtg tttttcatac tttcagaatc aagccagtga gttgccccgg
    18901 cggctgatac cacgttttct tcaatcataa tggagcccat gtcgtttgcc cctgcatgta
    18961 aagccagttg ggctgtacct ttgccaacag taagccatga ggcctgaatg ttggtaatat
    19021 tggtaagcat gatgcggctt attgctatca ttcgcaagta ttcgctacaa tcggtgaaat
    19081 tgtttatgcc catttttttc tgcaatactg tgccatcatc ctgaaaaggc catgcaatga
    19141 atgccgtgaa gccataagtt ccttcgggtt tttttgcctg ggtttcgcga agcttgacca
    19201 tgtgttccat tcgctcttca atggtttcaa tatgtccaaa catcatagtt gcagaggtga
    19261 caaggtttag tttatgtgct tcgtgcataa catcgagcca ttcctgactt gaacattttc
    19321 cgggagaaat ggctttacgc acacggtcaa caagtatctc ggctccggca cctggcaagc
    19381 tttccagccc ggaggttttc aattctgtta aaacatcact atagctcatt tttcccatct
    19441 ttgagatgtg tacaatctca gcaggcccta aagcatggag ttttatcatg gggtacatgc
    19501 ttttcagtgc agaaaaaagc tctttgtaat atgtcagtcc aagcttaggg tgcaacccac
    19561 cttgcaagag caattgatat ccgcctaaag ccatcattga ctctatcttt tcacgatatt
    19621 gttccaaagt agttatgtaa ctttcgggat gcccgggtat gcggtgaaaa ttgcaaaatt
    19681 tacaacgtgc aacacaaaca ttggtgatat ttacattacg gtctatcatc caagtcacga
    19741 tgtttcctgg tacatgcttt tgtcttaatt gaaacccaat atgcataaga tcttccatag
    19801 gagcatttct gaaaagataa gctccttcgt caatagatag aggctctagt tgtaatgctt
    19861 tgttatataa atagtttaaa ttcatcatag gtatttcatt gtttaaacaa aagaatcttg
    19921 cttatgttaa gttttacatc aaagtttagt ttattgtgca gttgtcacag cagtttgaaa
    19981 tagtaatatt aaaaacagat gggtatttct gtatgaacgg aaatataaca ttttgcaatg
    20041 cattgaaaac aatatgataa tcgcctgcca acactgtgct catggcatta gtggttatat
    20101 tgatgttttc agttttgttc agttccaata aaaacaattc aattacctcg ttgaaattgc
    20161 tgcataatgg caaatagctt atttggactt gaatgtttat catatttcct tatttagtgg
    20221 cttttacaac tataaataca ccatgtatat ctttattgac aatggtatca gggactaatt
    20281 caaatgaatc cgttagcatt acacagtctt ctataaaagt attgctaaac ttttttaaga
    20341 gttcgactaa atcctttttt gaaaagaagc gggcgtgctt aaaaataggg tcgttatctt
    20401 ttgtttttcc aagaaccgat tcgctattca gacagccaag tattaataag ccattttttt
    20461 ttaataccct gaacatttca tcaatagcat ttttggcgtt ttgagtaaat tcgagcattg
    20521 taacggatac tactacgtca taactgttgt catggaaagg taggttcatg gcatcgcctt
    20581 taataaaagt agcatggctg atatttttgg caagggcaat atcaagcatt gcatctgaga
    20641 tgtcaagggc tgttacacta aaaccaagat ttgcaaaagc cttggtccaa tgtcctgtgc
    20701 cacatccaac ttctaaaaag gttgatttga ctggtatgtt ttgaagatgc tttagtatgg
    20761 cggctttttc tatttcgtct atttttttac cttgcccggt ctgataatag gcatcatact
    20821 cattggcaat ctcgttgttt tcaaatacat tcatcgtata aaccactaat ataaaaacaa
    20881 aaggtaaaaa taggtcattt caataaaagc ttgcatggtg tacaaaaaga aacgctttat
    20941 atttttaaaa tgcaaagctt tggtaataaa caaaaaacgg ccactcattt gagcagccgt
    21001 tttccttttt tttgtaaaag tttatactgt agccagcacc actactttat tatttttcac
    21061 ctcaaccact ccctggcgga tatcaaaaaa gcttttttgg tcgtcattgg tcactatttt
    21121 tattttgccc gaagacaaag tagaaacaat ggctgcgtgg ttgcgcaata tctcaaaagg
    21181 ccctttggtg cccgggactt gaatgagttt tatattcccg ctaaatagct ttttctcagg
    21241 tgttataata tctaagtgca tcatagctta tcaattatca attactttga agctgccaaa
    21301 agcttttcgc ctttttctat agcatcttca atagtcccaa cgaggttgaa tgctgcttct
    21361 gggtatttgt ccacttcgcc gtccataatc atgttgaacc ctttgatagt gtcttcaatg
    21421 cttacaagca cacctttcaa accagtaaac tgttcggcaa caaagaatgg ttgcgataag
    21481 aagcgttgta cgcgtcgagc acggtgaaca accaatttat cttcttctga aagctcatcc
    21541 ataccaagga tagcgatgat atcttgaagt tctttgtagc gttgcaacaa cattttaacc
    21601 ctttgtgcag tttgataatg ttctttacca acaacttcag gggtaaggat acgtgaagtt
    21661 gaatcaagtg ggtcaacagc aggatagata ccaagctctg aaatcttgcg gctcaatact
    21721 gtagtagcat ccaagtgggc aaatgtagta gcaggagcag ggtctgttaa gtcgtctgca
    21781 ggtacgtaaa tagcctgaac tgaagtgata gaaccacgtt ttgtagaagt aatacgttcc
    21841 tgcattaccc ccatttctgt agcaagcgta ggctgataac ctactgctga aggcatacgg
    21901 ccaagaagtg cagacacttc agaaccagcc tgggtgaaac ggaagatgtt gtccacgaag
    21961 aaaagaatat cgcggccacc acttttttcg tccccatcgc ggaatgattc agcaacggta
    22021 aggcctgaaa gtgctacagt agcacgagct ccgggaggtt cgttcatctg gccaaacaca
    22081 agtgttgctt gtgacttaag catttcttct ttgtcaacaa cacttaaatc ccatccgcct
    22141 ttttccatgc tgtgtttgaa agcttcacca tattttataa ccccagattc aatcatttcg
    22201 cggagcaagt cgttcccttc acgggtacgt tctcctacac cggcaaatac agatgtaccg
    22261 gcatattttt tagcaatgtt gttgatgagc tccatgatga taacggtctt tcctacaccg
    22321 gcaccaccaa acaacccaat tttaccacct ttggcataag gttccagaag gtcaataacc
    22381 ttaatacctg taaaaagaac ttcagtttca gttctcaact gttcgaactt aggtggttcg
    22441 gcgtgtacct gataaccgcc ttttttgtct aatgtaccaa taccatcaat ggcatcaccg
    22501 gtaacattta acaagcgtcc cctgatttga tctccaatag gcatacggat agcagctccg
    22561 gtagctttta ctttggtgcc tctgcgcaaa ccatcagtgg agtccatagc aatggtacga
    22621 acggtgtttt ctccaatatg ttgttggcat tcaataacaa gcacttgccc attttctttg
    22681 gtaatttcaa gtgcatcgtg gatttttggg agctcttctc cctttgtgtc gaagattacg
    22741 tcaactacag gcccaataac ctgtatgatt tctccatata tatcagccat tgtatctaat
    22801 ttttaggtga tacaaagtta taattatttg ttgtttgtca tatttttttg aagttatttt
    22861 tttacttctt cgatattttc ttttaaatat aaaatactaa tatttaaccg tcatttttat
    22921 tgaatcactt ttaattgcta tctttgccga ataccagcaa cattagtttt catgaagaat
    22981 attgcattat ccaaaacaaa cagcatagac aacaacctga gtatagtggt tattgggaaa
    23041 aaatctgaac ccctggattt agttttttta agtgaagctg aaaaaaatta tttaaagcaa
    23101 aaagaggaag ccggaagttc ttttatagtg attaaccagt atatcaggtt ggtcattgta
    23161 gctcgtatag acacctcaaa agctgttttt gagcagcttg aggaagcccg gggaatgggc
    23221 aacagggctc aggctgagct tattgaaggc aagtataatc aggctttgat atatcattta
    23281 tttgatgaaa aagagaccct gcttgcattg gctgagggct ttttgcttgg agcttatcgt
    23341 ttttctaagt attttacaaa agaaagtgct aaagagcaga gtgtagaaca attattcgtg
    23401 tacagccagt gtgttcgaga aaaagaattg acagaactgt ccaatattgt tgatgcggta
    23461 tatgtcgcac gagatatggt aaatgagcct gtttctgagc ttaatgctga aaaattggct
    23521 caaactattg aagaacattg cacttcggca ggttgcaagg ttgagatatt ggataaagct
    23581 aaaatagaag cgttgaaaat gggaggactt ttagcagtaa ataaaggttc cattgatccg
    23641 ccttcattta ctattgtaga atggaaacct gataatgctg tcaatactca acctattgtt
    23701 ttagtaggca aaggaattgt ttatgatacc ggaggcctaa gtttaaagcc aactggtgac
    23761 agtatggatt atatgaagag cgatatgtct ggggctgcca ttgttgcagg tgtgatgaag
    23821 gctgctgctg ccaacaaact tccgttatat ttggtggctt tagcccctgc tactgacaat
    23881 aggccttctg gaaacgctta tgccccgggc gatgtagtga ctatcagtaa tgggactact
    23941 gttgaagtgc ttaatagtga tgccgaagga cgtatgatac tggcagatgc ccttgttttt
    24001 gctaaaaaat ataacccgca actggtaata gacctggcta cgctaacagg cgcagcgtct
    24061 gtggctattg ggccttatgc tttggtagct atgggaacag cttcagaaga ggtgaagaga
    24121 cgacttaatg agagtgcatt tcgtgttttt gagcgtatgg ttgagttccc gttatgggct
    24181 gaatatggag agcttataaa atcggatatt gctgatttga aaaacgtagg aggcaaatat
    24241 gccggggcaa ttactgcagg caagttcctt gaacatttta cagactaccc ctggatgcac
    24301 tttgatattg ccggggttgc ttttaataaa agtaaagaca gctatagggt taaagggggc
    24361 accggctttg ggttaaggct gctgtatgat tttctgaaaa aagaagcggc taaataaaat
    24421 atttgagctt ttccggtttt tctaaagata tttgctttcc ttcgatagga ctaccataca
    24481 aatcagaggc cagtcggtta aatatgtccg cagattcggt agtacagaag taacgtttgc
    24541 cagttttagt aactttttcg tccatttcgg gatggttttg aaggtatttg ctcaggcttt
    24601 cggccacaat gttgccttgc gaaacaattt ttactccctc aggagtatat tttgatattt
    24661 tcgaaaacag cagagggtag tgtgtacagc ctaaaacaaa tgtatcaacc ttgttattcc
    24721 tggacatcag ggcatcaata tgttttttta taaaaaaatc ggctcccggg ctgtcatatt
    24781 cgccgttttc gaccaacggg acccacaaag ggcaagcttc ttgctcaact ttcacattgg
    24841 gaaaaaattt ttcaatttct atggggtacg agttggacga aactgtgcca gaagttgcaa
    24901 aaatggctat attgttggtt tttgaaaagt tcccgatgca ctctgttacc gggcggataa
    24961 tgccaagcac tcttttgttt ggggcaattt ttggcaggtc gttttgctgg atagtgcgca
    25021 atgccttggc tgaagctgta ttgcaggcca aaataaccaa atggcaatct tgtgcaaaaa
    25081 gccattttac tgcttcaagt gtgtatcggt aaacggtttc aaacgagcgc gaaccgtaag
    25141 gagaacgggc attatcacca acataaatat aatcgtactg aggtagtttt tttactatct
    25201 cttgcatgat ggtcagtccg ccataccctg agtcaaaaat gccaataggt tgcatactgg
    25261 tgcttataaa aacaaaactg tcacctggta tccacccgat gacagtttaa aagtataaag
    25321 tttttttgtt atttcaaacc aagtttggct tttactaaag gcagaatatc ggtactgttt
    25381 tctgcatggt aaatgattaa gccacctgcc aaatcaaata tataagtaaa cccattttct
    25441 ttactaacat cagcaatagc tttattggct ttgtcaagaa taggtttgta aagtccactt
    25501 ctttgttttt gcaagtcttg ttctgcattt tgtgaaaatg tctggattct ttggtttaat
    25561 tcttgaagtt cattttcttt agtttgctta atcagatctg aataagtatc ccttttgctt
    25621 aggtaatcct gatatttatt gttcaattct acctgcatgg tttctaattg actttgcaag
    25681 tcttttgtca gtttttcaat tttcttttga gcgctatcgc tttcaggcat agcttcaatc
    25741 aattcctgtg aattgatgtg tcccaatttg atagctgtct gagctccggc agttgatata
    25801 aatgcagctg caaataagat gacaaggtat tttctcatgt ttatgtttgt atttttgtta
    25861 tttccgcaaa tgtaaccaat taatttttat atcccaattt ttgcaaaata gcatcgcttt
    25921 tgtccatttt tgggtcagta taaagcattg aagccccggc agagctgtca aatatcactg
    25981 cataacctcc gtcaacagca aattccctga tggccttggc aatttcatct tgtataggtt
    26041 tgataagctc ttcacgtttc ttgaacaaag cgccgtcttg tccgaagtat tttttttgca
    26101 ggtctttagc ttctttttct tttgccatga tttcttcttc gcggcgtttt ttcatttctt
    26161 cggtcaggag tactttttca gcctgatagt ttttgtacat tttatcaatt tcagcaaaag
    26221 cagcttctac ttctttttgc cagttttccg ataatttatc taactggtcc tgggcagttt
    26281 tgtaactggc aatgttactt aaaatatatt cggtatcaac gaatgcaaat ttttggctaa
    26341 accctaaagc tggtatagct gttaatgcaa gaaggatgat taattttttc atttgtcgaa
    26401 tattttaaat gagtaactta taaatttata attcatcaac tgctagaatt gttgtcccat
    26461 ggtgaactga ggttgccagc cggatacaga accattaatt ttatcaaatc cgtaccccat
    26521 atcaaatccg agcaagccaa gcattgggag gaacactctt acgcccactc cggcagctcg
    26581 tttgaggtca aaaggattaa atgaacgtgc atcggcccag gcatttccag cctctgtgaa
    26641 cgctgcggca taaatagtag cttgttcttt taaagtaatt ggataacgca attcaaaagt
    26701 aaaacgatcg taaatacgtc cggcagggct gggagtcaag gctccatcag aataaccccg
    26761 catcgggact atttcttcat tggtaatgtt gtaagtcatc ataccactac ctcccatatt
    26821 gtagctttca attggagcat aatcaatttt gttgttgtat cttgccagga taccaaagtt
    26881 tgccctggtc atcagaacga ggttgcctac aatagtggta tatgtttcag ctttaatggt
    26941 ccatttgtgg tattctgccc atttgtactt ttcggcagcg ttcatcttcg aatagtcttt
    27001 tccattcaag taagaatatg ggggcgtaaa ttgtacgcct atgctaaagt ttgagcctgt
    27061 acgtggatat aagaattggt ctagcgagtt acgcccaaac acaatgctat aactgatact
    27121 atttatagaa ccagtagaag tatctgaaat tatatagtaa tttttgaagc tatagttgtc
    27181 ataattgata gcattttgca aagtgaagta gtcgtcgggc caggtaagac gttttccaag
    27241 tccaatagac aagccaaaag tgttgatata ttggtcagac gattgtaaag cttgcgatac
    27301 agaactccat ctaacaggag tatagtgtgt gtatgacatg cttaccgata atgcatttgg
    27361 ctttttgttg ccaaaccagg ggtcggagaa tacggctgat aatgcacggt attgtgtccc
    27421 attggtacgg aaactgaagc taagtctttg gccatcacct gaaggtaccg gacgccattc
    27481 tttccattta aagaaatttc ggtaggagaa gttattgaag cttacaccaa tactaccaat
    27541 gaaaccataa taacctcccc atcccccgga tacttccagc tggtcatttg ccctttctac
    27601 cagtttaaat tgcaggtcaa cagtcgagtt tgcctgattt gggataggaa caggttcaat
    27661 tttttcaggt tcaaagtgcc ctaaggtagc aagctcgcgc acacttcgga caatatctga
    27721 tttgctgaac aattcgcccg ggagggtacg aagttcgcgt cgtaccacat gttcgttagt
    27781 ttttgtattt ccggttatga ttatatcatt gaaattggct tgtttccctt catatattct
    27841 catttcgagg tcaatagaat ctttgtcaat ttttgtttct accgggttaa cattgaaaaa
    27901 gaggtaacca ttgtcgaggt ataaagagct tacagcgtca tcgtcgcttt ggaggcgctt
    27961 gtccattaat ttttggttgt aagtatctcc tttttttatt cctaaaacat ctgacagcat
    28021 ttcagaagga tattttgtat ttcctaccca ggtaatgttg cggaaataat attttgggcc
    28081 ttcataaaca ttgatataca ggataatacg ctttgggctg ataacagtaa aggagtcggc
    28141 tattattttg gcatcgcgat aacctttttc attgtaataa tcaataaggt tgtttttgtc
    28201 gttttggtat tcagccaaaa tatatttaga agagttccag aatttccagt cacgcctttt
    28261 agtctttttc attgattttc ttagtttaga ggctttgagc tcattgttgc ctgtaaaaac
    28321 aatgtctttg atttttactc tttgattttt tgagattttt atggtaaggt caacggcatt
    28381 ttttctgagc gtatcgcttt tctggattat ttcggcagat acgttaaaga agcctttttc
    28441 gccataaaac tttttgataa taatctgggc ttcattcagt gcgttctccg tgatctggct
    28501 gcctggtttg aggttaagtt tttcacgtaa atcttctttg tcagatttgc tgactccttc
    28561 aatattaaac tttgacactc ttggcctttc tgtcagaaaa atatctatgg ccacgtggcc
    28621 gctggtagtg ggtttgtatg atacttttat ttcttcaaaa agtccatgtt cccaaaattt
    28681 tttaatgact tttgcaatgt catttccggg tatggtaatt tttgctccgg tagccaagcc
    28741 tgacatgttg gataaaaccc tctggtcgag gaatttaacc ccagaaatcg taactgtatc
    28801 aatgatataa tctttaggtt ttgaaaaatc tgctatctga acttgcgatg tttcctgagc
    28861 aataataaaa attggggata taagggacaa aaacaagagg acttttttaa tcatgagcta
    28921 tagtgtcgaa ttatttttta cttcacatta tcagagttaa gttgttcgct ggtttttccg
    28981 aaccttcttt cgcgtttttg aaaatcttta atggcttcat aaaaatgctt tttggaaaaa
    29041 tcgggccaga gtacatcagt aaaataaaac tcagaataag ctatatgcca caacaagaaa
    29101 ttacttatac ggtattcacc gctggtacgg ataagcaatt cgggggcagg cataaaaccg
    29161 gcctgtaaag cgttggtaaa tatgctttcg tcaatttttt cagggtctaa atggccttct
    29221 ttgacttcac gtgccacttt ttttaaggca tcaataattt cccacctgcc gctataacta
    29281 agggctagaa caagtgtaag cccggtgttt gacaatgttt tttttatcga atcctgaagt
    29341 tgctcataaa cattagcagg cagacttttc aaatcgccaa tagcctgaag ccggacatta
    29401 tttttcatta atgtttctat ttcgctatta agtgtagcga ctaataaatg catcaatgcg
    29461 tttacttctg atttgggacg gttccaattt tcagtagaaa aagcgtatag tgtaagatat
    29521 ttaatgccaa gttcggcagc tgcttcaaca gtatctctta cagctttgat agcatttttg
    29581 tgaccaaata tgcgttgatt gcccttcttt ttagcccacc tgccattgcc atccatgatt
    29641 acggctacat gtagtggcaa acggttttta tctatattcc cttcagtgtt catttaaaat
    29701 tctttcttat acgccggaca ttcttctatt gaattaaaaa tcttgtaagt aacaaagata
    29761 ttggcaaaat tataccagtc attattattt acaaaagtgt gcaagtttac atttttttta
    29821 tcaacaaggt tctctgtatt atctaaatta tcgttaaaaa gtttgcgaaa attccattga
    29881 acccctaaac tccagttttt cccgatgctt gtacgtaccc cgaaggcaaa agggaaaaca
    29941 aaattaatgg ttttgagatt tgattggctc attacaaaag ctccggcaat acctgaagaa
    30001 acaaaaggac tcaggtaata tttttgggca atgaatttaa tgggcataaa gttgaactcg
    30061 tactggattg cgccatcata taagggtaat gcaaaactat tgttgcggct cttttggtaa
    30121 atatcatcaa aactggcatc atttgcactt aatcgcatat aattagcttc aattttcaag
    30181 gcttgtcggg ggttaaaatt gtagcggtag gtaataccaa gatttatccc cgggctataa
    30241 aatggggtaa tataatttat atctccccaa taagaggctc ctcctatgca tatccccaag
    30301 tctgcgcctt gctgtgaaaa aaccatgaca ggcagcatga atagcacaat aacaatactt
    30361 tttttcatcc gggcaaaatt gattttcaat ggtcggatag cttgtcttgc aaaagcagta
    30421 gaatttgtcc cgacaaatcg ggatttataa gtgtttgtgt ttacttctaa acatgctttt
    30481 ttcataaaat actaatatct cttgaaaagc attggatatc ctttgcgtga tgtttttatc
    30541 ctgtatataa cgctaaaatt gagataatag tacatgtcgt tgaatttaga aaatttggaa
    30601 gtaagcccat cgaggtcatc acctatcaca aaacgaggcc cgaattctaa acttagtgca
    30661 agataattac tataaatggt ttttaacatt atccctccaa aggctatgcc ggtatatgaa
    30721 ggcccagagc tgaaatggtc gcctttataa gaaggatctg gattgctatg gtctataaaa
    30781 ggtatgtaaa ataaaccccc tacgcctgca aatgcatata agccaagtcg ggatcgggaa
    30841 tacataatcc ctcttttatt atatacagca aacgatgtcc tatttctgtc ttcagaaagt
    30901 agggcatatt caacttggcc agattgctca agcccgaaag tgttaaaact gtaattcctt
    30961 tgtggatatg aacctcgcga gtcatcgcca ttcaacatta taaatgaaaa agctacccgt
    31021 gtatggaatt tctgtttaat tttatacctg gcaccaacca tgaatgatgg ccttgtgtta
    31081 aagatacgta tatcttttat gccgtataaa ttgttattat tagaggtact tccaatatct
    31141 ccaaaatagt ttgaaacccc gacaccatac attagctcat accttgtcag tttccatttt
    31201 tgtgaaaaca aaataccgga aaccattaat aataagcata taagtccaat ttttcgcacc
    31261 agttcgggtt tttgaatttt acaaaaataa tattatttat aacattcaaa ggtatttttt
    31321 tgtttactaa tttctgacat caacccccca catgagttta tttctaatag tggcaaagaa
    31381 attgtggcct ggtaatctta caatattaat acagaaaggg gctttagata ttttgataac
    31441 agatgaggct gtcattagcg aatgttgggc atcaagtgta gcaacaaaat tttctgaacg
    31501 tgactttatt gataattcaa taatagcatt gtctggcact accattgggc gtacagacag
    31561 attgtgcgaa gctataggcg atataataaa tgctttgcaa tctgtagata ctatgggccc
    31621 cccggcgctc atagaataag cagtagagcc ggtaggtgtg gatatgataa gtccatcgct
    31681 ccaatatttg ttcaattcaa tgccattaat taaggttgtt actgtgataa gcgttgcgtc
    31741 tgacttttga atggtaatat cgtttaatgc ataaggaaat atagagaaag gattatattc
    31801 agactgaaaa gccagtaaag aacgtttctc tgtctcaaat gtcttattgt tgatacaatt
    31861 gacaaagtat tcaatttcgt tttttgctat ataagctaaa aaacctaaac gtccaaggtt
    31921 aattccggct atagggatgt ttgtgtcacg gacgtatctt acagactcaa gaaaagtgcc
    31981 atcgccgcca atacttataa aaagatcgac agacttggac aattgttctg gtttttcaaa
    32041 aacctgaaat ccaattttat tatgttgaat tatagaaata aggttttgat ggatagaaac
    32101 cttatggttt ccttttatca gcttttcaat caacattttt ttcaaatgtt catgctcggg
    32161 gttaacgttt tttgaaaaaa tggcaatatt catctcaaat gtttaggtat ttcataaata
    32221 gctcaaacct ttcgtcaagc agctcatcca tgatgttgtt gtttaaaaac gaggctttaa
    32281 tttgataatt ataacgaata aaagtctgga ctattgaagt taagtcggtt ttattaattt
    32341 ttaaagttac atcaatctga gttgaatcgg gatggggcga aacataagtg ctgagtatct
    32401 tggcattgtt gctttctact atttgcgaaa tttgcgacaa tgtataatca ttgatattca
    32461 tttcgagtac aataatagcc ccgggttcat ttacggcaaa caaagtggcc atttgcttga
    32521 gcaagtcgta ttgggtaaca ataccgagat agttgttttt ttcatccaaa accggtacaa
    32581 gagataatga taagcgtgaa aagatttcga taatctcgta agcatgttgg ttggccaata
    32641 caaaagggcg tgtgagcgac aaggcatgat tacctatagg ttcatcggcc atgttcaggt
    32701 caaaaatgtc tttatcagaa ataagtccga ggaactcgct gttgttgaca atgggcaaat
    32761 gtgatatacg gaaaatgtcc atataagcaa gcgctttaat ccctgtgtcg gaagtacgta
    32821 aagcagggac aatatctgat atcaattctt ttgccagcat aaatgaaatt atgataattg
    32881 gcttaccttt gcaaaggtac tatttttcaa taattttagc tatgaccagt ttaagtgtaa
    32941 atataaacaa aattgcgaca attcgcaatt cgcgcggagg caatgtgcct aatgttgttg
    33001 aggctgcaat taatatagaa aaatatgggg cccagggcat aactgttcac cctcgtccag
    33061 atggcaggca tattaagtac caggatgttt acgacttaaa aaaagtactg actacagagt
    33121 ttaacattga aggcaaccct acagattctt ttataaagct ggtttcagaa gttgtgccaa
    33181 cacaagttac tttggtccca gatgctcatg atgctattac ttcaaatgcc ggatgggata
    33241 ctgtggccca tctttcgttt ttgacatctg tagtaaaaga gtttaaaaag caaggtatcc
    33301 gtgtttctat atttgttgac ccaattgaaa aaatggttga atatgctgca gtagccgggg
    33361 cagacaggat agagctttat acagagccat atgcttccaa ttatttggac aaccgcacgg
    33421 atgcaatagc ttcatatata aatgctgcaa atatggcagt taaggttggc cttgacatta
    33481 atgcaggtca tgatttgaac cttcaaaatc ttaagtattt tgcccagaat ataccacaat
    33541 taaaggaagt gtcaataggt catgccctta tatcagatgc attgtatttt gggcttgaaa
    33601 aaactgtgca tttatatctt gattgtttga aatagcattg agtttttaga tattgactag
    33661 cctaatttgt tgattaatcg gggatttttg tttataagtt gcattctcct gattttgaaa
    33721 ctataaaaaa aagtctcatt tttgaaattg ttgcatatac cctaattaag cactaaaaat
    33781 gcttaatcga tttggaaatt gtattttaat aaacaatatt tcaaacaatt tttaaattag
    33841 cctgtctcgt tatgacggga gggttagccc aaataaatca tatagtttgg ttttataatg
    33901 gttaatttgg gataaactga aaattgtatt ctggaagtga taaattttac attataaaca
    33961 actaaataat atgagagtcc ccctgaaaaa agttgtgatg gcgttggtta tttgtttccc
    34021 ggccgtttta aatgccagca acacaaaaga aattgacagt ctgataagta aaacagaaac
    34081 tgcatccgga gttgaaaaag cttctttatt gaacgagata gctgaaaaat atttacctgt
    34141 tgatgctgaa aaggccgagt attacgccaa tcaggccaga gctttttcaa aaaccagcat
    34201 gagcaaatat aacgaggcta aaagttataa aataagcagc agcagttatc ttagtcgttc
    34261 gaggtataac gaagcgctga aagcatcgca ggaagctttg gatattttca tgaaagacaa
    34321 cagaacaaag gaagctgctg aaacctattg tcagattggc cagattcaca agcaaatgct
    34381 tgagtttgag aaatcaatga aagactacca caaagctatt gagctatacc agatgggacg
    34441 cgactcaagt ggagtggcga ctgtatattc attgattgga agccttttgc taaaacaaaa
    34501 ccagtatgat gaagctatta agtattatac cctggcgctt aaacaaaagc aacaggctca
    34561 agattctatg ggaatggttt cggtatattc aaacctttcg attgtcaaca gggataaggg
    34621 gaatttgaca gcagccattg agaaccttca gaaggctttg tctattcttg agaaaaaagg
    34681 ttcaaaagct gaattggcaa acgtatataa cttatttggc aatatttatt ttaagaaaaa
    34741 cgagtacaac aatgctatag tgaactatga aaaagctctt cagattaggg ttcagttagg
    34801 caataaaaca gaaatggctg gttcatacac caatataggg aatgtgtata aggaaatgaa
    34861 caattttcct aaggccatag aatatttgca aaaagcttat gacattcgca aagaattggg
    34921 aaatgccaaa gcagaagcta tagctttgaa tttcctgggt aatgcttatt ggaaagcaaa
    34981 gaaatatagt gaagcattaa gttactatct tgaatcgctt aagctgagtt tagagtcaaa
    35041 agagagtacc gagatatcat cggcatattt caatattggt aatatatact ttgagttaag
    35101 caattatgat aaggctttga agcattatag cgaagctttg gcgcagagca gctcatcaga
    35161 cgactatccc aagatggctt ctatttatca aagtatgggt aatgcttacc agaaaaccgg
    35221 gaaatataca gaagcattgg aatgtttcaa caatgcactc aaattgcgcc agaaattggg
    35281 cgataattta cagatagcaa ttagctatca tagtattggc ggggtttata ccgatatgaa
    35341 caacacacaa tcggccttga aatattacaa gctggctctt gaccttcgca aaaagatgaa
    35401 tgacaaggtg gccataagta atacccttaa tgcaatagga aatctgtatg ctgcagtaaa
    35461 tgataagcct caggctttgt catactttat acaagcattg caatatgcac gagatgcagg
    35521 ttatttcttc aatatagggc tttgttctcg taaaatcggc gaattatata ccgatatggg
    35581 agaaatagag aaggctttgc cccgtattac tgaatctgta gaaattggta aaaaattatc
    35641 caattatgaa ttattgcgca aaggatattt tgcattgtat aaactgtatg aaaaacaagg
    35701 gaaatataaa gaggcgctta ccaattatgt tttatcaagc cagtacaacg actctatcaa
    35761 tgttactatg accaacaaac agcttcttga catgcagtta aattttgaaa tgcttagtca
    35821 gcaaagcaaa gttcatcgtc aggaaataga gatagcactg ttaaagaaag ataaccaaaa
    35881 taaggagctg aatgctacca agaacaggct tcttattatt gttttgctca ttttgatgct
    35941 atttattttg tctttggtaa tattatatta taacaggttc cagatgaagc acaaaacagc
    36001 attgatgctt caggaaaagt atgatattat agagtcaaca aaccaaaagc tcaaacaatc
    36061 tgaagaagaa ctgcgcaaga tgaacaagac caaagataaa ttcttttcta tcatggcgca
    36121 tgatattaaa aacccgctgg ggggaatggt gaccattact gatatggcaa ggactgattt
    36181 tggtaacctg gctgacgatg ataaaaagga aatgtttgaa gctattaaca agtcttcaaa
    36241 gcagctttat actttacttg agaatttgtt gcaatggtcg cgttcgcaga ctggtcgcat
    36301 accatatagg cctgtagaat tgcagctttc agaaatagct gagaataaca ttgaattgca
    36361 gaaggccaac gctaacaaga aaaatatcat tgttatcaat cttatcgaga acacgctgaa
    36421 agccaaaggg gatttggaaa tggtaacact catcatgcga aacctcattt ctaacgccat
    36481 caaatttacc ggagaaggtg gaagggtggt tatatctgca aaacaaaagg ataataaggc
    36541 tgagatttgt gtagaagata atggtactgg tatcagcgaa gagaatttgc aaaagctgtt
    36601 ccgcattgac acacaacttg taacacctgg cactaacaac gaaactggta caggtcttgg
    36661 gttgattttg tgtaaagaat ttgttcagaa aaacggtggc gaaatttggg tagaaagcga
    36721 agaagggaaa ggaagcaggt ttttctttac cttaccgctt gcatagctca tagatgaacc
    36781 gatacttaac cctcattgca gcaatatttt gttgtagctg tgcttttgca caaaaagtag
    36841 ttgattttga ggatcggcgt tttgatgcat ggcattcaat tccagaaggc cgatgggagc
    36901 tgacaaatca agcacctata caaggaaagt attcattaca tcatttattt gataatgata
    36961 aatcagggag agattatatt ttcttgcttt atcaagatat gattgattgg aatgatacag
    37021 ttacctggtc atttaaggtt aagcatggtt ataatccttc ttcatccaat aattgggcta
    37081 tttcttttac agaacccgac acccatgttg ttgaaccatc atcagattgg gcaatagtgg
    37141 ccggagttaa tcagattggc agtgatgaca tgttaactct ttatgccatt aatggttttc
    37201 aaaccgttaa gattatttca tcccagttta attgggagac aaaaataggg acttctgttg
    37261 gatatgttaa ggttagtcgc actccggatg ggatgtggtt gttatgggct ggcaatagtg
    37321 aagattcggc tatattggtt ggacaaggtt catttgtaga gccaattaag gccacttgtt
    37381 ttagcatcag ctatgaatat tcgtcatctc aagacaggaa gctttgggtg gacgatattc
    37441 attttgacat gaaaatgata aaagacattt ttgcccccaa ggttgataca gcttatgttt
    37501 cgggggcaaa agagtttacg ttgctgtttg acgagcctgt tttcggagct aagcaaagta
    37561 atgactttgt aaaagcttta aataatgaac atttaattga tagtagtata ataaaagaca
    37621 ataaaataaa ggtttatctt aaagatccca ttgcatgtaa ccaacattat tgttttttgt
    37681 ttacaggcat tcaggatatg cataataaca ctgccagaga cacaacaatt tgtttttatt
    37741 tgttcaagcc aaaggctttt gatgtggtgg tctcagaaat tatggccgac ccttctcctt
    37801 cggccgggct tcccgaattt gaatatcttg agctttttaa cagaagcgat acttcaataa
    37861 acctggagaa ttggcagatt catgcagata ataaaatggt gaaattgcca aaagttgttt
    37921 tggagaaaga ttcattcctg attgtttctt acaaaaatgc caagggggtt tttgacaaat
    37981 atggaaaaac tgtgtattgc ctgcccgatg tttattttct caataatggc ggggcaaaca
    38041 ttagtatagc cgattctaca ggccagatgg ttagctattg ttggtatgct gacagttggc
    38101 atactgatgc aataaagaaa aaaggcggat ggtcgttgga atgggctgat aaatcagatt
    38161 tatgcggggg tagttactca tggaaagctt caaagtccaa aaccggaggt actccgggta
    38221 agaaaaacag tgaaggaagc actaaagtaa acgatattaa aataaaacct tctaaaattc
    38281 ttgttcctaa tgacagcaca ctgatagtgt cttttaataa gccggtagat atagacgctg
    38341 tgttggacaa atctttattt caaataggac tatctattgt atctgttgta aatgtcaaat
    38401 ttggggcaaa tttttattca cttgagcttt ttttatctgg gcatttagag aaaggagtta
    38461 cttatacgct caatttttct gataaaatct gtgattgcaa tggtaagcct attgtaaaaa
    38521 cagaactttc gtttgttttg ccacttgaag ccgatagcaa tgacttggta attaatgaag
    38581 tgctttttaa tccctatgct ggaagcactt attttgttga gttttataac aggtcagata
    38641 aacctttgga tataggaagc ttgtattttg ccaggtttga cactttgacc aacgaagtat
    38701 ataatttttg cagtatttct gaacaaggtt cttttctaat gcctcataaa ataatggcta
    38761 tttgtaaaga taccttgtta ttgtctaagt tttataagtt atatgataca tgtgtagtat
    38821 tgagcaatcg tgattttcct tcgttagata acaagcaagg acgtattggc ataataaaca
    38881 ggcaacagca aataattgac cagctaaact attatgaaga ttggcatttg ccgtttttgt
    38941 ccagcaaaga aggtgtttcg cttgaacgtt taagtagtga agctttgaca aatgatagaa
    39001 acaactggta ttctgcggcc gggacgtatg gttatgctac tccggggtat gccaattctc
    39061 aaaagcaggt taataatatt acgaatgacg gtattatcag cataactcca gaggtattct
    39121 cgccaaatgg cgatggtttc aatgatttta cgaccattga aattaaacct tcagaaactg
    39181 gggtgcttgg agcaatcaag atatttgatt ccaatggaaa tatggttagg acattgcttc
    39241 agcagcagct tatatctgaa aatgataggt ttgtttggaa tggcctttca gacaccaaca
    39301 aaatactgcc ttccgggatt tacattgttt ggttgaggac gatttcgaaa aatggaaaag
    39361 ttgacgaacg ctacaaggtg gtgactttgg gcaatcactg atttttatta tattttgcta
    39421 taacagttgg taatttctcc aataaacatg tggtgaaaat ctccttcggg gtatatttct
    39481 tcagctattt ggggaacatt gaagcattct tttttcagtt ctgccgtgta gagctttttg
    39541 cattcgatta ccagcctggc ttgctcaaaa gtaatggctc ctgatggcat aaagataggc
    39601 ttcagcccgg tttctttgat tttatcaaca tctttgcctg aatatttgcc gcagtaactc
    39661 agcttatctt tgtgttctgg ataaaggaag cttaatgtaa acatatcaga aatttctaca
    39721 aacgagagcg tatgccgctg aggacggata aagatagtag ctacaggttt tttccataaa
    39781 acgccaaaag aaccccagct ggcggtcatg gtgttaagtt tactggcatt tccggcagta
    39841 atgagcatcc attctttgtc taaaagttga aatacattgt cttttaattt gtaggggtct
    39901 gttttttgaa aattgtccat gcttgactat ctttatatgt tgaacaattg tagtttatcg
    39961 tttagcaaaa ttagtgtatt ttgcagattt ttggagctta aaaccgggca aattgaaact
    40021 ttccattttt aaatattctt tgtggtacag tattgtcagg ctatacgtaa aagttgccta
    40081 ttatctttat tatagcaagg ttgaagttat cgggagggag aatattccta aaaacaggcc
    40141 ggttattttt gccgccaacc attccaatgg actaatggat gctttggcat ttgtatgtac
    40201 agtaaaccga caggtagttt ttctttcgcg agcagatatc tttagttctc cttttatatc
    40261 aagggtttta aatttcttta aaatattacc tgtttataga cagcgggatg gaataaagag
    40321 tgtatcaaaa aatgaagaaa tatttcggat tgtaaaagaa ggactttgta tgggtaaggc
    40381 attatgcatg atgcctgagg gtacacatca tggaggcatt aaattgcttc ctttacagac
    40441 cgggatattc agactggcca ttgagacagc taaaatgcgt gggggagaag atgtaattat
    40501 tattccaaca gggctaaggt atgaacatcc caaacgcttt aggtcaaaag tatccattat
    40561 ttttgggaac ccgatatctg tgagtgaatt aatgcatttg agtgaagcga atgcaagtaa
    40621 attgttgaaa gatatgctta ccaattcgct tacccgactg gcgggatata ctactttgcc
    40681 agattcggag cttgacgttg ccaactttgc tttgaatgtc ccaagtgtta aggcagtgaa
    40741 aaagtcggag gttataaagt atagtaatac atgggtatta aatcggtttg aaaatatttt
    40801 caataatagc ccgatatttt ataaagaatt atccaggaat gtcagtgaat ataatgagtt
    40861 gcttttacga agtaaaataa agccttttta tttattacaa tttcaaaaca gtattttttt
    40921 aacagcatta agtgccctac ttttattaat tactttgcct gtttttataa taggtattag
    40981 ttttcatgca ttaccattgc tatttgccca tgttttaaac ctgaaaaatg aagattacct
    41041 ttttcattcg tctgtcaggt ttgttatttg gctattatgg ttaccgttcc agtttacaat
    41101 gctgacattg gtattgattg ccattggaat aagcatatca acattattag ccatcttgtt
    41161 tatgattgta atgagcggta ttttggcact tttttatttg gatattctca gaaatgctgt
    41221 tgttaacata gcctgctttc tcaatctgag aaataccctg atagtaaaag caaagcaaaa
    41281 gaaaattatg ttaaaactct ttagtaaaag ataatttggt gacaggtcaa tacaaggtga
    41341 tatggattac aggggtctcg tcggggcttg ggcgtgaatt ggctgtaaat tatgctgaac
    41401 ttggtgtgtc tattattatt tcgggacgaa atgaagcaca tttggcagaa acagctttat
    41461 gttgcaaaaa taaaggagca gagatattat ctctaccttt tgatatgact attgatactg
    41521 aaattgaagg agctgtaaag ctggctatag cctggaaggg gaatatcgat ttgctaatac
    41581 tcaatgcagg aattgggcaa cgtgcttatg ttcatgattt gtcaatggat gtagccgatt
    41641 atattatgaa agtcaactat cggagcttag tgtatataac caaattactt cttccccggt
    41701 ttcttgttca aaaaacagca catatagctg ttattagcag tgtaaacggg ctttatgggt
    41761 ttcctttaag ggctgtttat tcggcatcaa agcatgcttt gattgggttc tatgaatcac
    41821 ttgccttaga aatggcttca aaaggtattt gcgtgacaat agtctgtcct ggccgttttg
    41881 gttccggatt ttcaaccagg gctttggatg catttggtaa accttatgaa ttgcttgata
    41941 atacgcataa aaaccttttg agtgtggaaa aaactgcgct aaaagtgatg agcgccattg
    42001 cccgtaaaaa gtatttttgt ttttttggtg gcaaagagct tatattgttg tatttgaagc
    42061 gtttaaattc gaatttattc tttagtttta ccaaacggct taacccaaaa tcatagatta
    42121 tgttgaaaaa cctgccaatt gaaaaggtgt tatttctgga cattgagaca gtaccaatga
    42181 gtccggactt tggacaattg cctgacatat tcaaatcttt atgggataaa aagtccaggc
    42241 atttcagaaa agaagaacag accgcagagg aggtttatga acgagcagga atttatgccg
    42301 aatttggcaa ggttatttgt atttgctttg gcactatttt tcagcgcgat gggcaatggc
    42361 acacgcgtat tcgccatttt gctggtgatg atgagaagga gatacttttt cagtttgccg
    42421 aattgttaga aaagcttgaa acacagggag gttggcgttt gtgtgcccac aatggcaaag
    42481 agtttgattt tccttatatc tcgagacgaa tgttggtcaa tggcattaag ttgcctgctt
    42541 tgcttgatgt taccatgatg aaaccctggg aaacatcatt tcttgataca atggaactct
    42601 ggaagtttgg ggattacaaa aactatactt cattggaact cctggcggcc ttgtttggca
    42661 taccttctcc aaaaaatgat attgacggga gccaggtagc taaagtatat tatgaggata
    42721 aagaccttga acgtattgta aaatattgca aaaacgatgt cctgaccatc atccagcttt
    42781 tcctcaaata caggggagag cctttaattg agaatgagag gattgactta ggggttagcg
    42841 agtgaaaacc acccctgcct cccgcctgag acgggacaag ctctcctttt aaaggagggg
    42901 gaatatggat aattgtaatg aaagtaaaaa ggataagaac aattgaccga attttaggaa
    42961 tcagattttt ttagttcttc gttatacctt tttgaaagca aatcgagcat tttgctcatg
    43021 gtttctatag cttctacagt agctggcgaa gcttcttttt tcgagagtct tgctaacata
    43081 agccaataca tagcttcgag gcacacatga gtgtcgggac gagagctttg cggtgatttg
    43141 gctttgtacg atatgatatt aggctgcgcc agaataaatg cctggatata aagcccgtca
    43201 ttgttcctta gcaattgttg gtgaaactct tcaatttcat tcatcacatt gataaccact
    43261 tgtatatggc cagcagtttc aaccttttca ttacgcatca tttctaccag gctttcaatc
    43321 caaagctcag tatttttaac gtcttcctta tttgtatcag attcttttat cagaaatgct
    43381 tttgctttgg aggtgtcaaa gtttatagcc ctcagcaaat cttccatttg ccagagataa
    43441 agtacataag ccgcaatatt ggtttgtttg agatgatgag ctaatagcat ttcttagttt
    43501 ttagattact cccaatccct tattctgtca atctcgcgac ggtctttttt tgtaggcctg
    43561 ccagcacctt tatcacgctg aaaattaaca gccatgcggg caatttcgcg ttttacaagt
    43621 tcgtcttcag gcgtaaggtc ttcaatggcc tcttttgcct taggtgcaga aactctgctt
    43681 ttgattacct caataacacg gtatgtatga acaacaggag gtttacggac gataatcact
    43741 tcgccttctt tgactatatg cgaaggcttg acctctgtgc cattgataag aacacggcct
    43801 ttttggcact cttcagcggc aatggagcgg gttttgtata tgcgtacagc ccaaagaaat
    43861 ttgtctatcc tgactctctc gtccataacc ggagcacttt atttatacaa gcaaaggtaa
    43921 ggagtatcct ggtcgcattt aactttaaac tttgaatctt tagcaacaac aaatgattca
    43981 aactttttat atgttttcca ctcagtttct ccggcttgtt tcacttgcat caccccggca
    44041 attactgtca ttacttcaac agttgaagtg ccaaattcgt actcgccagc agcaataaca
    44101 cctatagttg ttctgccttc tacagtttcg cagcctaaag aagctacttt tccaccaaaa
    44161 tattcgtttg ctttaaacat gtttttattt ttttgcaaaa gtagccgata tatactaaag
    44221 tttcaatgaa tggcgtaatt aatcttttac ataaatattc atttacatca aaggttaaaa
    44281 atttttacca tcaaaaattt tattatgtac ctttgttccg tttttttaaa atttaaacca
    44341 ttgaaaaaca gagttatagt gatagtgggt atttttgcaa tactcatgat ttcatttttt
    44401 gttgtgcgta agccaattgc taattatgct ttaagtaaag tccacgataa aattgagtcg
    44461 cggttgcacc taaactttaa agttgaaaaa gctgagttca aaggtttgct ggctcttaag
    44521 ttgtctaatg tttctatcac tgattcatta atggatacaa cgttgtatgc caatgaaata
    44581 cttgtgcgcc ctaagatttg gtatttgttt acaggaaaac tcaggcttag agagcttcat
    44641 atcaatcaat cttacattaa cattgatagc agtattgtct cctctgtatt aaagcataaa
    44701 aaggatgaaa aaacagacac tttagttgtt gaggtaaact ataagaaaac agtggagcgg
    44761 attttcggga ctttgttttc tcttatacct aaaaaagtgg ttatttcaga ttgtcgaata
    44821 acgattttgc ccaagaaaat cactgtttca gttcctgtgt ttaccatagt caaagacaaa
    44881 atgcagggaa acatttcagt taatgatgat gttttaaaaa actatttcag tattcatgga
    44941 gaaatggata gccattgggt tagctttagg ctttttaatg acagtattga agctgatcat
    45001 aagaaagtga cagaaatgaa tgaccgttgg ggcatggggt tgatgtttga tagtataact
    45061 tcgctattgc attacaatgg tgtacaggat ggtcgggccg aggtagtttc agaatttagc
    45121 ctgttcaact tttctgtatt tcataaaaag ctttcacctg tgcctgtttc gtttagaaaa
    45181 cttcagatga gttttgattt taaggcttcc gaacagacat tatttataga tagctcgtca
    45241 gttatcagtt ttaacgggtt tactttttct cctttcgtaa aatattctcg tacagaccat
    45301 aaataccttg agctgggggt gaaaaatatg gaattctgtg ctgatagttt tttcagggct
    45361 ttaccagaag gattgtttga gaacattagg gatataaagg ctgaggggaa attagcttac
    45421 aggttgaagg tcaatattat cttagatact cctgacagtg taaaagtatt gtcagatttg
    45481 gaaagtaaaa aatttaggat tgttcgttat gggaatgcta acttctcgat gctgaacgat
    45541 acgttccatt atacatttta cgatcatggg gttcctacat attcgatgct tgttggacaa
    45601 ggtattggtg attatactcg gcttgaccaa ataagtgact ttttgaaata tgctgttctt
    45661 acatctgagg atggcgggtt cttttatcat cgtgggttca atgtagatgc cattcaagag
    45721 gctattgctg aaaacattaa aaagaaacgt tttgttcgtg gggcaagtac cattagcatg
    45781 cagctggtta agaatgtttt tctgacccgc aataaaaaca tttcccgcaa gcttgaggaa
    45841 ataatgattg tttggatgat tgaaaacctg cacctcgtgt caaaagagaa aatgtttgag
    45901 gtatatctca atattatcga gtggggaccg ggagtgtttg gcattaagca ggcggccaat
    45961 tattacttta gcaaaaagcc ttcagagcta acacttgaag aaagtatctt tttggcagct
    46021 attatccctt ctcctaaagc tttcaaatat tgttttgatg aaaataaaca gttaaaaagc
    46081 tgttacgatg gttattataa gatgttaagc aacattatgc taagccgtaa ccagattact
    46141 gttaccgata cagccgggtt aaaagcaaat gtaatactga aaggcagggc tttagaagct
    46201 ttgaaaaaag acacagctat ggttgttgat tctgttgaaa ttgatgagcc tttcatgttt
    46261 cttactgaat aaaagaaaag cctgtcaagt gctgagctga caggcttcac aaaacaacat
    46321 gatataaacg aaacttattc tttaaacctt ttagccactt cgttccagtt gattacattc
    46381 cagaaagcag caatataatc gggtctgcgg ttttgatatt tcaggtagta agcatgttcc
    46441 caaacatcca atcccagaat tggtttgcct tttacatcag ccacgtccat caacgggttg
    46501 tcctgattgg gagttgatgt tactgccagt gatccatctg cttttacaac cagccaggcc
    46561 cacccgcttc caaaacgcgt tgcggctgca tttgacagtt gttctttgaa tttgtcaaaa
    46621 gaaccaaatg ttttgtttat tgcatcggcc aaatctcctt ggggaacccc gcctccattg
    46681 ggcgacatca ctttccaaaa taagttgtgg ttataaaaac ctccaccgtt attccgcaca
    46741 gctacaggat aggctgatat gtttttaata atttcatcaa tattgtccgg agccgaagga
    46801 attgcagcca atgcattatt caggttgttg atatatgcct ggtggtgctt ggtatggtgt
    46861 atttccatcg ttaacgcatc gatatgcggc tcaagtgcat tgtatgcgta gtctaattta
    46921 ggtaattcga atgccatagt atttcatttt taagtttaac ttctgtattt attagttaaa
    46981 catgggaaaa tgagttttgt ttaatttaga ttaaataaaa ataataaatt ttaaaaaatg
    47041 atacaataac ttgaaaaact gtgtgttaat tcacaataaa tttaaagaga gaatttttac
    47101 aaaacggttt tagaaatatt attaaccttt ggagaattaa tcgaatcaac ttttatatca
    47161 tctccagcac cttccttgct atgccttctg catcaaaacc gcacagatgg tgcagctcag
    47221 ctacagtgcc atgctcgata aacttatcgg gaacgcccat acggacgatt tgtcctttgt
    47281 atcctttttc catagcgtat tctatcacgg cagagccaaa tccaccaaca atagtgccgt
    47341 cttcgatggt tattattttg ccaaagcttg aaaaaacttc atctaagata tctgtatcta
    47401 tgggttttac aaaacgcatg tcataatggg cgggtttaac ctggtgttct gtctcgagct
    47461 tttgaatagc gctttgcaca aagttgcccg gatggccaac agataaaatg gctattttct
    47521 ctccgtcaga aacttttcgt cctttgccaa tagccatttc agtaaatggt tgtatccagt
    47581 caactgttac acctgctcca cgaggatatc gtatagcaaa aggcccgtag tttttatgct
    47641 gagcggtgta catcatgttg cgaagctcaa cctcattcat tggagctgca atgataatat
    47701 ttgggacaca tcgcaaaaag gccatgtcaa acactccatg atgtgtagcc ccgtcttcgc
    47761 ctaccaatcc gccacggtca aagcagaaaa ccacctgtag cccttgcaaa gctacatcgt
    47821 gtattatctg gtcgtaggcc ctttgggcaa aagatgaata tatgttgcaa aaggggacaa
    47881 gcccttgggt agccatgccg gcagcaaaag taacggcatg ttcttcagca atgcctacat
    47941 caaagcagcg atcgggcatt tcctgcatca tgatgttgag agagcatcct gtgggcatag
    48001 ctggggtgat gcctactatt tttttgtttg ttttggccag ttcgagaatg gtaaggccaa
    48061 aaacatcctg atatcgtggc ggtttatttt tattgtccga actggccact atttcacctg
    48121 tttctttgtt gaactttccg ggggcatgcc aaatggtttt gttttcctct gcaaacttgt
    48181 agcctttacc tttggtggtc atgatgtgca atagctttgg cccgggaata tctttcaggt
    48241 cagaaagtat attgaccaac tgcctgatgt tgtgcccgtc aacagggcca aaatagcgaa
    48301 aatgcaacgc ttcaaacagg ttgccctggt gcatcaggat tgacttcagc ccattctcaa
    48361 ttttctggac agcagagcgc gttttggggc caaacttatg taaacgcccc atggcattcc
    48421 agacgtcggt ttttattttg ttgtaagtct tggaaatggt gatgtgcgta aggtaatcgt
    48481 tcaaagcccc tacattgggg tcgatggcca tgttgttgtc gttgaggata accagaatat
    48541 tggagtttag atgcccggcc tggttgaggc cttcaaaagc cagccctccg gtaaggcttc
    48601 catctccaat aatggctact gtccggcggt ctttatcatt gttcatttta gcagcagtgg
    48661 ttatgccgag agctgctgat attgaggtag aagcatgtcc aactccgaat gcatcgtaaa
    48721 tactttcttc acgttttgga aatccgctta atcctcccat tttacggtta gtgtgaaaaa
    48781 tatcttttcg tcctgtcagt attttatggg cataggcctg atgacctgta tcccacacta
    48841 tttggtcgta aggagtattg taaatgtaat gcagggcaac ggctaattca acagctccaa
    48901 gacttgcacc aaaatgcccg gggttggtgc acatctcatc aatgataaac tcacgaagtt
    48961 catcacataa tgcattgagc tgtgtaacct gaagcttttt caggtctgag gggaatttta
    49021 tttggtcgag cagcggatat gggctgttac tcatcttaca ttcaaaattt ttcaaagata
    49081 aaaattatat aacgaaagtg tcacgcgttt tcgtttattt tccctgcgct tgttgttgga
    49141 aatgcaaagc gttaagggaa agcataaagg ctgtatagaa aagcaaaagc tttggcaaaa
    49201 aagtaaacgt aatttgttaa gttcctttat ctttggccga aacaatataa aacagaatag
    49261 atggaattta aagagttgac agaatctgat ggctggaaaa aatttgaaat aaaacttgag
    49321 aaacttggag cattagtact tttggtggga ttgatagtgc atttactgaa tgttaatccc
    49381 aacaggccaa attacttgct ggttgttggg ctttcaacac ttacaatagt ttattttttc
    49441 tgcggtttca aagaattgaa atctactgat tatgtatcca gtatcttttt caggatttat
    49501 ggatggggac tggcaataac ctctgtgtcg cttatgttta ctttgatgca atggcctata
    49561 cacaaaaaca gcctgcttat ttctataggg cttttggcgg tatcgttaat attggcattg
    49621 aagtttagaa atgaggagaa caagcaacag ataaactata ttttttttat caggataggt
    49681 gtagccctgg ttttggcagg tgtggtctat tatacaaagg ttatgggcgc ttattgataa
    49741 ttaaaaccgt aaagaatact gagaataaac acggagggct gtagcgggtg caaagttgat
    49801 aattctctta gtttacttat ccaaccagtt tttaaactgt gtagcccgca gtttgcttac
    49861 ataaatttct ttgtccgaag caggtttgac ttgtattttc agcttttgcc ggccaaagtt
    49921 gtggatcttt tcaatacttt cgacagataa aataaattgg cggttggccc tgaaaaacgc
    49981 tttggcattt agttcgtttt ccaattggtc caacgattcg ctgatgatgt ggcgtttgcc
    50041 gctttttgta gttaaaaagg taacgccatc ttccgattca aaataggcaa catcgttaac
    50101 cataatagga ataaggttgt cgccatgata gacaagaaaa cgttcacgat attctttatt
    50161 aataaagttt tttaaaatgc tttcgaagtc tgttgcctgt ttttcctgga gaatggattt
    50221 atacttgttt aaactttcct gcagatcagc ttggtttata ggtttgagca ggtaatcaat
    50281 gctgtaaaac ttgaaagcat tgatggcata ctcatcgtaa gcggtagtaa atatgatggg
    50341 ggttttgatt ttcagttggc taaatatctt gaaactcaag tcatcggcaa gctgaatatc
    50401 gctcagtatc aggtcgggta ggggattagt agaaaaccat tcgatgcctt ccttcacgct
    50461 ttgaacaacg ccaacaattt cggcgtttgc atcacattcc tttaccagct tcatcaaccg
    50521 gcgtgaagcc ggcgcttcgt cttctataat aagtatcttc atttgtttac attaactcat
    50581 tttcaaattt actagttatc tcattccttt taccagcggc acttttactg taaacgctgt
    50641 ttcggtatct tccacaatca cttctttatt ggccaggaat cggtatcgct cgttgatatt
    50701 gtgcaggcct actttggtgg acgatggcat gatgttacgc ttttgcttgt tattgcagac
    50761 aatcaaaaac tcatcgctgc aaacgatgtt gacttccagt tttttttgca ctgatatttc
    50821 gttgtgtttt acagcatttt cgatgagcag ttgaatggta agtggcagta tataatatgt
    50881 ttggtgatgt tcgcaaatgt caaggttcac tatcaggctg tcttcaaacc tcatccgcag
    50941 caaattgatg tatgactggg taaatttgat ttcagtcggc aggtcaaccc atgtttcctt
    51001 ttgactactg agcacatacc tgtaaacgac cgacaattcc tgtatgtatg acactgcttt
    51061 tttggagtct tcttcaacta tagcagtgag tgtgtttagc gaattaaaca gaaaatgcgg
    51121 gttaagcctg ctgttgaggt tttgaaaccg tgcttcggcg ctctcctttt tgtatcgctc
    51181 attttcaagg gctgtatggc taagtttcag aaataggtaa aagccttcat agataacatt
    51241 gataaggatt acaaacagga gggttgcaat aaagaaattt ttgaaatcaa agctgcttgg
    51301 gtcacaaagg gtacctgttt tctcgtatcc atagctatgg ttgaaataca taaaagaata
    51361 atctaatgaa atggatatag cgcaggagag caggacttga agtgcaaaac gaaggtactc
    51421 gtggttttta gggaaatggc gcctgaaaga aaaaatgatg tatcgaatga gaaaccaaat
    51481 aattataatg ccaaccaagc ctgaagctaa atgataaaaa aacaacttgt tgaacacaaa
    51541 tggataaggg gcaataaaca atgttgcaat gacagcaaat aagggtattg caataaactt
    51601 aatccgtttc tcgaccttga taaattcagc ttccaagtgc tcttcgcaaa acaacataat
    51661 cttttcttta agtaataaat tttgtcagtt cttttcaccc caaaccccta aaggggcttt
    51721 gtccaagtcc cctttagagg taattacgtc taaaaacaat gcctttctta atatttacat
    51781 caaattaaaa atactttctg gtcagcatca aaggtataat tattatcagc aataacagca
    51841 agttgaaaac agcgttgaga taaaccttgt aatagataga aataatttca tctattggga
    51901 accaggccaa tactgatatt aataatgcct tccaggccca taattgttgc tttttaaagg
    51961 cattgtttat caccatcagc atcaacgtgc accatgctat cataacagcc ccggtaagcc
    52021 catagatgaa gccctgaaag ttcctggctg ccatatcagg ctcattgcct gagccccaaa
    52081 aaggagtatt gacaatgcta ttgaaaataa agctgtcgct ttgattgaaa aaagccatat
    52141 atgccccggt ggctatcatt gcaaccatta cagcattgag ccaattgtag tagaatttga
    52201 agtttttcat tttttttgtt tttcattttt ttcaagcttt aaattccacc ccctgccccc
    52261 gccagcgggg gatagctatt attagctttt atcttaattt gacagatagt tctatttaat
    52321 aataacagat taacaagaat gataactgtc ccccgctggc gggggtaggg gtggatttat
    52381 caacaaagaa caaatatgtc ataacatttt tggtatccgt aatttgtcat cagtcattaa
    52441 aagtatatct tatactgaaa cgttgggaat atccccaatt ggtagatatt ccccagttgg
    52501 tttttgctgg catcgtaggt ttgggcaaat atgttctttg tattaaaaac gttgtccaca
    52561 ttgacaaaaa cctcctgagc tttgcccaat ccgttgaagc gataagtgac cttaatgtcg
    52621 gttcggaaat agtccttgaa ttgctgtgta taagcctgac tttcaatacg cacggctttt
    52681 ttagccagct gtgatgcagc ataatcaatc ggggtatagc gtttgcctcc cgcagttgtc
    52741 actttgaagt ccaccattat ttttgaacgt tcattaatct taaactcctt tccagccaat
    52801 acattgaaaa catatttccc gttaaaggcc gtattcctta gtactccgtc gctgcctttg
    52861 tatttcgagt caaagagcga cgctgttacc agataatagt agccattgcc gaagaacttt
    52921 tctaaagtta gttcaagccc gtaattgcgt cctgtaccat tgtttacaag gctgtcttcg
    52981 gttgtaaatg tatatgacat accttcgtta atggccgaaa aagagcttgg cctgctgttg
    53041 ctggttacag gaatatcaaa caggtattgg tagtatgttt cggtttttat ggctgtactg
    53101 ttggttatca tccagttata gccgaggaca aactggtggc ttttgttgaa cttcaggttt
    53161 ttattggtat tgaggatatt gccactgata tttgtagaaa ccatgtatat tggcaacggc
    53221 tgcatctggc tgtgcagccc gtaaccgagg gtaaaagcgt gttgaggcct gaaattccat
    53281 ttcagtgagg cccgtggctc aatggcctgg tcgttgttta gctgtaagta ttgataatgc
    53341 aagcctgttg taagcagcag gttgtcggta aatgaatagt tccattgtcc ataagcctgc
    53401 aatagtgctg cggctccatt accgtcatat ctgtcaagca ggttgccgtt ggcatcagca
    53461 tcaaacttag tatcaaagct gtaattatac ctgtctgcga taatacctac ttttatcttg
    53521 ttttgtgcgt taattttgct ttttatgtaa gtatgcaggg tgaatttgta ctggtagttt
    53581 tcgcccctat accttgggta ttcttcataa ttaataaggc ctacggagtc ttcacgtcct
    53641 atttcgtata taccgctcat cccggctatg gtttttatat acgaattatc ggagattctg
    53701 tgctgatagg atattccact agcgcctgta ctcgaaatcc atttggtatt ggtatttaaa
    53761 tagctatcag ggctaaagtc ctctttttct ttgtctttgt atagtacctt gtagatgctc
    53821 ctgccaccca ttccaaaaat acccagagtc cctttagctg aaacgggcag gtttacattg
    53881 tacgaaaggt cctgatagtc tgcctgtcct gcatatccaa agttaatatt cattaacctc
    53941 aaaatggaag tagttgaaaa acgatagttt acaagatacg aacctttgta gtccttttta
    54001 tacgggcctt ccatcataag ttcagtgcct agcacccctg cctggaacat atattcatgc
    54061 ttttggttat taccattgcg cagcttaata tcaaaaaccc cggatgtggc attgccatat
    54121 tcggctgcaa atgcgccagt cataaagtca gagttagaaa taacgttgta attgagcata
    54181 cttaccgggc ctccaccact cccaacactg gtaaaatggt taggactggg tatttccatt
    54241 tcttcaagtt tccataacag gcccataggc gagttccccc ggataacaat gtcattgcgt
    54301 gagtcgtcgc cgtttgtaac cccggcatag tttgaagcca tacgcgacac gtcattgcgc
    54361 gagcctgcat aacgaccgga ttcttctacg ctaaaagtgc gggcgctaat gctgttcatt
    54421 tcgttcagcg cttgggtttt gtttctgtgg cctactacag ctacttcctg cagggtggtt
    54481 acgctttctt ccatgccgat attcaaaaca agctctttgc cagagtttat ttctatatcg
    54541 cttgcagaag cttctttgta tcccaggaaa ctaaagcgca aattctgtct tccaaccgga
    54601 acctggcgaa ggacaaactc tccgttggca tcggttgatg tgcctatgag tggattagta
    54661 ccttctacaa ctactgtcac tcccgggagg ggcattttgc tgtctttatc aatgatgaca
    54721 cctcgtacag attgggtaaa acgttgagca ttgataaaag ttgtggctgc aagcagaata
    54781 gctaccatta aaaaattcag tttcatgtct tttattgttg atttcagggc aaagttttgc
    54841 cgaaatcaag gcccaaaaaa tgaaaatgcc atgaagtgga tataagtggg tatgaaatgg
    54901 tatttttaag taaaaagcaa aactaacaga gaagattttc aatcataatg catgatgaca
    54961 attagatatc aattattttt ctatttttga aagaattatt taacaactga aaattatgaa
    55021 gacggcaaat aacctggtta agcttgtatt gttgagtgtt gcatttagtg catgtgtccc
    55081 gcttcaacgt tttcaggata cccaggataa gcttaataca tgccagacag agcttgatga
    55141 aaacaagcaa aagtccgagc agcttggggt tgaaaatacc gagatgaagc aagtgattga
    55201 aaagaaccgc cttcagctca acaaccttgt caaggacagc atgaaccgta tgttggtcat
    55261 tgataccctc agggcacagg tgtcaaatct tcagcttcag gttgccgaac tgcagaatac
    55321 ccaggaatct atccttaaag gcaatgccaa agaaacttca cgcctgttga agcaattgca
    55381 agccacgcag gaagacctga aaaaacgtga agcggcattg caaaaggctt ttcaggaact
    55441 ggaagaaaag aaaaacagcc tcaatgtgct caatgccgag attacccgtc ggaatgtccg
    55501 tcttaaagag ctcgaaaata tattgttcag aaaggactct gtggttaatg ctttgaaagc
    55561 aaaagtttcg gctgcgcttt taggttttga gggcgaaggt ttgactgtaa aagtcaataa
    55621 tggcaaggtg tatgtatcta tgcaggacaa actgcttttc aaatcggcca gtacagaggt
    55681 ggacaagaag ggtcaggatg ctattaaaaa gctggcaaag gtgttggaac agaaccctga
    55741 tatcaacgtg actatagaag gacataccga caatgtacct tataaatctg atgaaagaat
    55801 aaaggacaac tgggatttga gcgtgcagcg cgctacggcc attgtgcgta tcatcatcaa
    55861 taactcagac gtagctccca aacgcctgac agcttccgga cgtggcgaat tttcgcctgt
    55921 tggctcaaac aaaactgctg aaggccgtgc ccagaaccgc cgtaccgaaa ttatccttac
    55981 gcctaagctg gatgagcttt ttaaaatact ggagagtaac tgaaataatt acataaatgt
    56041 atttttctga ctaaaagagt tacaataatg taactctttt tttattttaa ataatttgta
    56101 aaatattgat tataaatatt ttacatgctt tggcatagag gttgaaaaca ggtaagtata
    56161 atctttaata cctgtaatta tgaaactaag tgcacgcaac aagctcaagg gcaaaattgt
    56221 caaagttgaa gaagggctta tcacagctaa agtattactt gacttgggaa acgacaacta
    56281 tatttcggcc atcatttcga aagatgccat atccgatttg cttttaaaac ccggtgatac
    56341 agcttttgct ataatcaaat ctaccgaagt cattattggt acgccatgtg attgcggaca
    56401 aggcaaatgt caatgtcatt agttatgttc caggtttctt gcaaagtggc tattagcaaa
    56461 gatggggaag cctttcttac gcttgaaaac atccaacttc tcatagaaat tggagaaact
    56521 ggcagtttga tggcagcggc aagaagcagg caaatttctt atatgaaagc gtggacaatg
    56581 gtaacaatgc tgaacaaaac atcaaaatac cctgtggtac tgatgcaacg gggagggcat
    56641 cagggtggag gcgctgaact tactgaatat ggcaggaggt tactggctga ttacctgcag
    56701 gttgaaaagc aagtaggtaa cttgttggct caaataaaca tggaacttag cctttaaatt
    56761 ttttttgacc acgttatcta tttcatttta tatcgaaaat attttaaata tggaaactga
    56821 cattatttac aaaccaatag gttttattaa aaccccgttc gaaaaggcag ccgaaatgcc
    56881 cgtgcaacct cagggggcaa ctggtataga aggcgttatt gaattgaatg cggaatacgt
    56941 cgccgggctt tccgacctcg atggcttttc acacctcatt ttgttgtatc atttccaccg
    57001 tgtaaagggt tacaagcttc atgtagtacc gtttatggac gacaagccgc acggcatctt
    57061 tgctacacgt tcgccagtac gcccaaatcc tattggtatc tctattgtaa aactctgcaa
    57121 agtggaggat aacagggtgt tttttgaagg ggccgacatg ataaacggtt cgccattggt
    57181 tgacattaag ccctttttcc cgaaatacga caaccagcaa aacgtgagag gaggctggct
    57241 cgaagcaaaa ggagatattg atataacgaa agttaagtcg gacagcaggt tcgacacaga
    57301 taaaaacccg gggttcaatc gctgcaaatg atatgaatgc cccaggctat taaaacaaag
    57361 tgagttttaa catttcaaaa ttacaaaaaa tgaggaagtt ttacgctttt atgggcattg
    57421 gtttaacaag cctgtgttgt ttgtccaagg ttaacgggca aagtacgatt gatgcagagt
    57481 ttcggccccg taccgaatac cgccagggtt tccgcaaacc gcttgccgat acgctcaatg
    57541 cagggtttgt aaccttgcag cgtacccgcc tgaacgccga ttacaaaggg aagatactga
    57601 atgcccgctt atcgttgcaa gatgcaagga tttggggcaa cagcgacaac aaaacaaatg
    57661 cttcgaagat tgagttgtac gaagcctggt tcgaatattt tattacatca gggttctcgg
    57721 cccaaatggg tcggcaaccc ctgaaatacg acgatcaacg tttgttgtct gccccaaact
    57781 ggagcaatac cgggctggca cacgatgctc tggtgctgaa atacaacagt ccgtttatac
    57841 aggtgcattc gggttttgct tacaacaata caaaagatac cctgatgaac gttgcctata
    57901 aatacacccc gaaacaaaac tacaaaacta tggggtatgc ctggctttca aagcaaattt
    57961 atacaggtac aaccctttcg ttgattggaa tttgcgaagg gtttgaaaat aaagtggatt
    58021 acaacgtagt ttatccccgc ataacttacg gaggtaacct cgtgtatgcc aatgattcaa
    58081 cggcgtgggg cgctacttta acgggttaca tgcaacaggg caaagatcct aacaaagccg
    58141 ttggcgacgg ttatgccgat ttaaaatcgt tctttctggc agccaaattc tcttataaaa
    58201 tcattcaaaa actatcggca aatgttggtg ttgattatta ttcgggttcg gatgctgcca
    58261 tagatgccga aaaatcgaac acgttcaacc gcctttatgg cgcaacccac agttttaacg
    58321 gcaacatgga atattttgta acactgccaa cgcaaggttt gattgattac tacggcggag
    58381 taaccgcaat aattacccct aaactttccg cggacctgtc ggggcattta ttctatttcg
    58441 acaaagattt tatttataaa aatgagaaaa cagacaaaaa cctcggcacc gaattagatg
    58501 ttgtgctgaa ttactcggta tcgaaagaaa tagccataca gggagggtac agccattatt
    58561 tcaactcggg cacaaccatg aaatattaca aaatgcaagg cgtagaagca cacccacagc
    58621 agtgggctta tgtaatgctt accatcaaac cgcagtttta taaaacacca cagaattaat
    58681 ttgaaaatga aacaatttga agataaaaca atttgaaaat ttgaaaataa atgaatttga
    58741 aaataaatga atttgaaaat aaatgaattt gaaaataaat gaatttgann nnnnnnnnnn
    58801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa atgattaaca cattttcaga ttagcacatt
    58921 aacacattaa atatgaagag aaacatcaaa aatacgtgga gttttgtttc gggaacaatt
    58981 attggcattc ttggaggctt gatagggctt ggcggggcag agttccgatt gccgctcctc
    59041 gcaggatttt tcggtttcgc cagtctcgat gcggttatca tcaataagat aacgagcctt
    59101 gtggtagtgt cgtcctccct tcccttccgc agccagtcta tcccgtgggg cgatgtggcc
    59161 tcgcactggg gcatcatcgt caacatcttg tcgggcagcc ttgcaggtgc gtggttaggg
    59221 gcacattacg ccacgcggat gaaaactgtt gtcctcgacc gcttcatcat ggtcttgctt
    59281 atcgcccttg cttttgggat gattttcggg cacagtgtca tggatggtgg gcacgctgcc
    59341 atagttcagg caggtacagc ccggatggtt tcgggcgcca tagcaggcgt gttgattggc
    59401 atggtggctg ccatcctggg tgtagccggt ggcgagttga taatccctac gctggtcatc
    59461 cttttcgggg tcgatataaa gctcgcgggc agcttgtcgc tttgtataag cctgccaacc
    59521 atgattgtag ctttctaccg atatactcaa agcggagcta ttgaaatatt caaacgagaa
    59581 aagaaatttt tgctgtggat gatcgcaggg tctatcatcg gttcgggcat cggcgggcgt
    59641 ttgattggca taataccatc gaatatcctt gtccttgttt tgggtggcat cttgttagtt
    59701 tcggccatta aaacatttaa gaaaaaataa tagagaatgt gaaaatgagc cgatttgaaa
    59761 atttgaaaat gagaagaatg accccaagaa ctagtggaaa aacagtttgt agagtaataa
    59821 tttaaaacaa aaataatatg aaaaaagtaa gtttcgcatt ggtaatggcc ctgagcgtgg
    59881 gccttcatgc acaaaaagta aaagtggcgg ctgctgccga ccttcgttat gcgatggacg
    59941 atattgtcaa ggcgtataaa gctgctaagc ccaatgccga tatcgaggtt atttttggct
    60001 cgtcgggcaa tgcctacacc cagatatcca atggggctcc gttcgatatt tatttctcgg
    60061 ccgacatcat gtacccccgc aagctgaaag aagccgggct tgccataacc gaacccaggc
    60121 tttacgctat tggccgtatc gtgttgtgga gctccacgct cgatgtgagc aagggtttgg
    60181 gcatactgac cgagaaacca aacataaaaa ttgctactgc caaccccgag catgcaccgt
    60241 atggcgagcg ggcagtagag gctttgaagt actacaagct tttcgataag gttgagaaac
    60301 aaatgattta cggcgagaac atctcgcaag ctgcccagtt ttgcctcacg ggcaacgccg
    60361 atgccggctt gctggccttg tccattgtgt tgtcgccagc catgagtacg cagggcaagt
    60421 actacctgat tgactacaaa acgcacaagc cccttgagca agcttacgta atattgaagc
    60481 aagccaaaga caatgccgag gcgtatgctt ttaccgagtt tgtggccacc aaacctgccc
    60541 gcgagatatt cgagaaatat gggtttacat tacctaaata atttgctaat ttgaaaatgt
    60601 gccaattgga gaatttgaaa atgaattcat tgcatatttt caaattataa tactaagctg
    60661 gcgcgcgttt gtaacgcgtg ccaatacctt aacttacaat gaattttata agttaaaatt
    60721 aattcagttt taaattttaa aaagatgaac aaactaccgg gcaaaataac acagatgcaa
    60781 cagtcgggag ccgtgctgtt ggtcgatgtc gatgttgacg ggcagggttt ttcggccttg
    60841 ctcatcgaac cggccgcgag gcccgaatgg ctgcaagccg ggcataccgt gcacctcgtg
    60901 tttaaagaga cagaagtgtc gctggccaaa aacctctcgg ggcttatcag tatgcgaaac
    60961 cgcatgatgt gcaacgtttc gaaagtggaa cggggcgaat tgctgagcaa aatcacgttg
    61021 cattttcggg gacacagcat tacttcggca atcacaaccc gtgcagtgga ttcacttcaa
    61081 atcgcaattg gcgacgaagt agaagcaatg gtaaaagcta acgaaatatc gctcatgaat
    61141 tcaatttgaa aatttgaaaa ttgaactaat tattgaaaat tacctcattt tcaaattggc
    61201 tcattctcga attttcaaat tgccacatat tcaaattatc tcattttcaa attaataaat
    61261 tatggatgca atttttgccg aaacgttgtg gataacctgc aagtttgcag ttttcaccac
    61321 gttaatttta atagtcgtag gcttgccgct ttcgtactgg ctggcctact caaagtttag
    61381 gttcaaacca ttggtagagg cgctggtgag catgcccatg gtgctgcctc cctcggtaat
    61441 tggttattac atgctggtgg cctacagccc ccgtcattgg tttggtggtt ggcttttaga
    61501 cacgttcaat gtgcggctgg cattcacctt cgagggcatt ttgcttgcct cggtcatttt
    61561 tagtatgcca ttcatggtgc aaccgctcca aaacggtctg cgggccttgc ccgatagcct
    61621 gcgtgaggct tcatacacgc ttggcaagtc gaaaatggtt acttttttgc gggttcttgt
    61681 ccccaacatc aaaccctcga taatcacggc cattgccctc acctttgccc acagcatagg
    61741 cgagtttggc attgtgttga tggttggggg caacatgccc ggcgaaacga aggtagcttc
    61801 tatcgccatt tacgacgagg tgcaagcact gaattttgca aaggcaaacc agtatgcgct
    61861 catcctcttc agtatctctc tccttttgtt aacaatcatt tatagtgtaa ataaaaaaca
    61921 cgacccatgg aaagcaatgt aattaccctc gaagtagaaa aaatgatgct tactgccaac
    61981 ggcaagctga acctttcggt cgatacccgg atacaaaccg gtgagttcgt agccctgttt
    62041 gggctgtcgg gtgccggcaa gactacgctc ctgcggatat tgtcaggtct gatgaaaccc
    62101 gataaaggct tggtaaaatt tggtgacacc gtgtggttcg attcatcgag gggaataaac
    62161 atcccgccac aggagcggaa catcagcttc atgttccagg actacgcact tttccccaac
    62221 atgacggtag aagaaaacat acattttgcc cagcccatga aagacaatgc tacggctctc
    62281 gaattgctct ccatttttgg gttgtccgaa ttccgcaaac gcaagcctgg cggactttct
    62341 ggaggtcaga aacaacgtgt ggcccttgca cgggcattag cgcgcaaacc ccagttgctg
    62401 cttctggacg agcctctctc tgccctcgat tctggcatgc ggctaacctt gcagaacgag
    62461 atatacaagg cccatcagct tttgggggct accaccatca tggtgagcca cgaccttggt
    62521 gaagtattcc ggctggcaag tcacgtgctg tgcatcaaaa aaggccagat aagcaaagaa
    62581 ggcacaccag acgaggtatt ttcagattca agcatcagcg gcaaggtaca aattaccggt
    62641 caggtggcac gtattgccaa acaagatact gtcaatattg ttacagttat tgccgggggt
    62701 agccaaatca tcaaagttat agctttcgac agcgatatgg aagccctaaa tatcggcgat
    62761 aatgtattgg tatttacaaa ggcattcaac ccgatgttgg ttaagttaat ggcttaattt
    62821 ttttctggca gacatttata tttaagttct gcagtaaaag aggtttttcc tgcagtttgg
    62881 ttaaagccat gtatttttat caggttgttt ttttcaataa aaaacaagtt tagctcatcg
    62941 tttagcctgg cttcatgttt ataattgatt ttgacacagc acaaatgctt ttggcaattg
    63001 gtttcaaagg gcatgtagtc aaaaacccac tgcaagtatt taccattgtt ggcatgctgg
    63061 ttaaggtcca aatcggaggg ccggactttg tgaatagtgt tactgtagtt gtttttagcg
    63121 atagagatat tggcaggttc ttcatccaga aactcagcat tgaggtacgc tgtttccatt
    63181 accgttggga attgttcgat tttgcgggtc tgggtattta tggctaagta ttgaatggaa
    63241 gctgctgcta taaggttgag cttggcatct ctgatagtaa attcacgcaa agcatgcaat
    63301 tgttgaggtg gacgtaacca ggtttcgaca ataatgtaat cgttccatga agggaggcag
    63361 cgcaatttca aaagcatttt gtggatgaca aatgcagtat tgtttttata catttcgcgg
    63421 tatccggcct ggtattgcat ggcatgttgc caggctgctt cctgtaagta gttagataac
    63481 gaagtaatag ttgctttacc gctagcatct acttctccaa aattaatatg aaatgctgtt
    63541 ttgtatagct tttcgggcga tggaggatgg tgtatatagg catattgttt acttaccata
    63601 aagcttggtg tatttgcgtt gacaaaacat ccgggtttgt ttccatccaa tatggctgta
    63661 ataatctaat gcattggtgt tggtaatatc tgcaagcagt tgcatgcgga caacgccaat
    63721 tctttcgccc caatcctgca tttcgtcaat cagttttttg cctatccctt tttttctgta
    63781 cccactttca actaccatgt cctcaagcat catagatagt gcgccttctg cggtagatat
    63841 agaaacctgg ccttgtaaca tcccgactac tttttcatca atggtggcta ccattacgca
    63901 tgctttagga taagctttta tcagcattct aagacctttt gtctgtttat cagcatcaaa
    63961 attaaagtct gcttcaatag aaaataactg ttccagaagt tttaccattg ctggtatgtc
    64021 gtcttttgtg gcccgccgaa tgagaatgtt attttccatt ttcttgaagt ttagttttat
    64081 ttatctgtaa gaatttgata aaagtaatac ttcagcatct gtcaggctgc gtcttttgca
    64141 tactgcagtt gttttcactt ttttaagaag ctcctgaagc ttttgtttat caaggtttat
    64201 agaatttttc tcgagcaaac tgtttaaggc attgctgccc gaatgaatac caaaaacaaa
    64261 ggacgagcca ctttttccaa cttcacgcgc atcgaaaggc tcgtaagccc ttctgtcgcg
    64321 gagcatacag ttggtgtgta tgcctgattc gtggcggtgc gtcatttcgc ctactattgg
    64381 tttatctgcc ggcagcttgc gttctgaggc ttctgctgta aaggtgcata attcgtttag
    64441 gtgttcggta gagtatggga cctgaatgcc catggatatt ttcatagcca ttattacttc
    64501 ttcaagggca gcatttccag cgcgttcgcc aagcccgttg acagtagtac ttacgcagtc
    64561 agctcccgct tgtatagcac tgatgctgtt tgctgttgcc atgcccaggt cattatgccc
    64621 atgaaactca atttgtaagt tgctgtcttt tactttcgac acaacacctt ttatcaggct
    64681 gcgcgtggta agggggttga ggcatcctac ggtatctgca atgcgtaccc tgtaaacccc
    64741 taattttttg gctatgttga ggtacgaatg cagaaatttt ttgtctgccc ttgaagcatc
    64801 ctgggcccca agtgagacat actgaaattg cttctttgca taactaacaa tttcaggcat
    64861 tgacccgagt acccattgcc tgtctttgcc cattgacaaa agctggatat ctgatacagg
    64921 gtatgaaata ttgattcctg ttgcaccagt ttttgcggca gcgtcaatat cttcgatttt
    64981 tgcccggctc cagcatgaac agcggaaaga aaacttataa ctgaccagct tttgggccat
    65041 aatcaaatcg tgttctgaaa taaagggcga acctatctcc agctcttcaa tgccgatttt
    65101 gtcaagcatg cttgctatgg taattttttc gttgaggcca aaaactacgc ccggcgcttg
    65161 ttctccatcc cgtaaggttg aatctataag gtaagggtgc ttcacctcca acccctcccc
    65221 aggggggaga ggtgaagaac aagaaaaaat ggaggtgttt tttttcatcc gtaaataatt
    65281 tttttaataa ggtcggatgg aactcgcatg atgaattttc atccttgttt gtgtttaacg
    65341 aatcatgatc gtttcatatt gataaaactt aatatgattg gactgataaa acttcaattt
    65401 ttgaatgtgg ctgcattaat cagcaataga atatttttcg accagctcgt tgttttccaa
    65461 ggcgtccagt atttcgtcta ttgcatcttc gctgatattg ccataccaga aattgttggg
    65521 gtgtaccacc attactggcc cctgggcgca tacgttgagg catccggtgc tggacacagc
    65581 agcatcaatc cccctgtcgc tgcacccttc catgatgtat tggatgaatt gtgctgcgcc
    65641 ttttttgtta catgccccct gggcatcacc tgcaacgcgg tacgagttgc acactaaaat
    65701 gtgataagct ggctttttca ttgtattttg tttttttggt taatatttat attttaaaaa
    65761 cactgaatat attgtttctg tgaggatttt gacatgaata ccccttgact gtatatattt
    65821 tttgggcgtt tcaccaattg atgcagtata aatcaatgaa cagtcgtgca atgcacttac
    65881 aatatgttcc aaagcttcgg gatttatttc atggttattt tcctttgatg aatacgccgt
    65941 tattcgtcgt attccgataa agttgataaa tccatcgctt acatcataga tgtatagctt
    66001 gtcagctttt ccaaaatgct tgtcaattgc tatgccgtct gatgaagtaa aagctatttt
    66061 cattttttta cagtatcaag tattaattat tattttttgg aactttttca aggtccatca
    66121 tatctgcatt ttagtcttta aaatccttct tatctccttt aaaaaggggg cttcttatcc
    66181 acaaccacca ccgctgccac tgcagtcttc gccacatttg aaaggcttgc atgctgtaag
    66241 cccgtttagc gatgctcctt tatatatggc atcaagcccg gcttctatga gcccgttcat
    66301 ctggattatc ttaatgccca tgcttttgag cattgaagaa gggttagccc cggcgccatt
    66361 gaccagtata gtgttacaat cgctcaggag cttggccagt tgtatccatc tgatgtcccc
    66421 taatccggct ttgggcgctt cgcgtgtatc tacatgaatg tagccttctt tggtgttggc
    66481 aaaaatcagg aagtctttag cttcgcccag gtgttggttg accaatatac cttcgtggct
    66541 tgcaacggct acataaggtc tgttttcggt ggcaaacaag ggcttgttgg ctgcttcttt
    66601 aagcagagta actgtatctg caatatcttt tcctaaaagc cctgctgcat cagccctaca
    66661 acgagcacag tgtttcatag gtttgatata ggccgatatt tgctgttgta tgtcatgtac
    66721 catttgtttt gaaggttctt caatatcttc aaaagcggtg tctttattag ggtgtacagg
    66781 tatgcagttc atcaggtcgg cacccagcga ggctacatac tttgctactt ctacaatgtg
    66841 gtggtcgttg atgcccggga ggatgatgct gtttatcttt accgtgatat tgtgctgctt
    66901 gagcagtttt atggcttctg tctggcgttc gataatgagt tttccggctt caatgccccg
    66961 gtatatcttt ttgttataac gtatccacga atatatttgg gctgcaattt cggggtcaat
    67021 ggcattcatg gtaatggtaa cgtgcgaaac gccaagttct acaattttat caatatgttg
    67081 ggcaacatct atgccgtttg atgacaggca atacagcatg tccggaaaga cagttttgac
    67141 ttgcttaatg gtttcaaagg tttcgttgac attggcaaag gggtcgcctg gcccggctat
    67201 ccccaccacg gcaatttcaa cacctttgtt gcttatcagg cgcatgtatt ctgttgcctg
    67261 cgaaggagcc aatatggcac tgctcacccc tggcctgctt tcgttgacac aatcaaattt
    67321 acggttgcaa tagttgcact gtacattgca atttggagct actggcaaat gaacccttcc
    67381 gtatttaccc ttggcttctt cgtcaaaaca tggatggcga tgaagttttt cttctgttgt
    67441 tgctgtgttg ctcatctttt ccgtttttaa tatattattg ctttaaggcg tttgtcttac
    67501 atatatttat accccaccgg ggaattgttt tgtttatatt ctattaaagc attgcaaata
    67561 gaatcaaata gttgcgtggt acctttgtag cctaagtgca atattcgctg gcctcccatg
    67621 cggtcgtgta tcgggaaacc gaccctgacc aaggggatgt taagttcacg ggcaatataa
    67681 taacctttgc tgttgcctat catgatgtca ggctttagtt cgcgcgacaa gctggcaatg
    67741 gtttcaaaat ccattccttg cagaatggta agcttgctgt tgccggtcag ctggcggata
    67801 cggtcctgag ctttgccact ctccccgcca gtggcgcaaa gcacaggctc tataccaatt
    67861 tcatcgagaa acatcatcat ggcaataaca aaatcttctt cgccatacac tactgctttt
    67921 ttgccaaaca cgtatttatg cccgtctatg taagcatcta taagtcggcc tcgctctttg
    67981 gtatattttt caggtgtttt gatttgcgca ataccttcaa gcttctcaaa gaaaaggtct
    68041 gtagctttta ttcctatggg catgccaatg cgcatattgg gtatgccaaa gttgttttgt
    68101 aaaaaggctg ctcctgttgg gtttactttt gagtcgttta cgctattatt tatatttccg
    68161 aggttgagta tatgcccaaa ctcaatgctg gcttgtgctg acccggtttt ttttatatca
    68221 tttactgaag tgccgccggg aggaatgcga tgatactctt cccagtttgg gttgtcgagc
    68281 gattcggaat agtcaggcag gataatggcc tctatcccaa atgcctcaag tatttctttg
    68341 aggtgacgga tatcttcagg cgaaacaaag cccgggaaaa ggttgatatg cgagcgttta
    68401 aaccgtcttt ctgcaaaggc tgcaactatt gaggctacgg cttcatgaaa cccgtccatg
    68461 tgtgtcccct ggtagctggg agttgagact gccacaaaat gcggtagggg ttcatcttta
    68521 tgttgcgctt tatatgcaga tatcaggcct ttgatatctt cgccaatggt ttcgctcagg
    68581 caagtgctgg caatggcaat tacctcaggt ttgtattgcc tgataatgtt gtcaatgcct
    68641 ttgttaaagt ttctgtgccc cccgaatatt gtggtgtcct ggctaaagtt ggacgaggca
    68701 atgtccactg gctctttata gtggctgatg aggtagcgcc tgatatacgt cgaacagcct
    68761 tgcgagccat gtatcagagg tacgcatcct ttaatacctt tgaaagcaac acttgccccg
    68821 agtggagaac acactttgca agcatttgta gtggctgcgg ctgtggtatt ttctttatat
    68881 gtggctgttg ttttcatggg ttattttttt tattaaaact tcgtggtttc cagaagtacg
    68941 tgagtcttaa cttactttta agccccggtg atatttataa ggtgcgtttt cttcctgcga
    69001 aatttctatt tcgctctgtg gagtgtcaaa aggcatgaac cctttggtgt aggttatttt
    69061 catctttctt tttgcgacca aaagtttatc ttctttcttt tcaatagtct ttcggccaag
    69121 gccgcgaaca ggattgatat atttgttccc acttgcgtca taaatggaga agcttacaaa
    69181 aaagctttca atcctttttt tgaaaggttc cttctgaacc catgtttcaa tgtattgttc
    69241 caaaaaagca tccatttttt tatcttttaa tgaattgact tgtaaataat tggttttgag
    69301 ttacttcaat ctttgttctt ggttctttat tcttggttct tcctgagaag ggctttttct
    69361 tacaaattgc cagacagggc tcatggccgt ggcgtatatt tctttggcga agttgagcat
    69421 cccctcatag cctgccagtg cttctttgcg ttcgtggtta tggtcgcaaa agcctatgcc
    69481 cagcttatgt gctataggac gctctttcac cccgccaatg aatattgttg cattcttttc
    69541 acgaatgaaa tgttgcaatt cgatggggtt tgaatcatct acaatgatgg tcccctcatc
    69601 gcatatatct tttataaggt tataatcgtc tttattgccg gtttgcgaac ctatcacaac
    69661 ggtctgcacg ccaatcagcc ttaatgcttt taccagcgag atagctttaa aagcgcctcc
    69721 tacatatata gcagctttct ggccggtaag tttttctttg tattccttca atgccgggag
    69781 gagttttttc agttcggttg ctaccaaaag ttttgcagca tccattttac tttcatcgtc
    69841 aaaaaacctg gctacttcgt aaagggcatt gctcatatcc tcgataccaa agtatgatac
    69901 gcggatatac gggatgccat acttttcttt catgtctttg gccagccagg tcattgaacc
    69961 tgagcattgc actacgttga ggcaggctgt gtttgccctg cggatatctt caacacgtcc
    70021 gtcaccggtc acctgggcta tgatgttgat gcctattttg cgataatatt cggccagcat
    70081 ccaaagctct ccggccaggt taaaatcccc caatatgttg atagagaaag gtactttgtt
    70141 tctttgttca gatgtaccaa taagtttaga aagtgcttcg catgcaaccc tgtacccgtc
    70201 tttttttgta ccattgaaac cttcggcatg tacaggcagt acggtaatgc ctttttctgc
    70261 gcttactttt ttgcacacag cctctatatc atcgccaatg acccctacta tgcaggtaga
    70321 gtaaacaaaa gctgctgcag gtttgtgttt gtcaatgagc gtggttaaag cggcataaag
    70381 ttttttttca cctccaaaaa tgacttgttc ttctcttaaa tcagtggaaa aactcaaacg
    70441 atgtagctgg ggcccggatg ataaggctcc acgtatgtcc caggtatatg aagcgcagcc
    70501 tattggtcca tgtacaacat gcaaagcatc agcaatggga taaagcacta cccttgaacc
    70561 acagaaaaca catgctcttt ggcttaccga ccctgcttca ctggcttttg aacattccat
    70621 atcgaagttg gcatcaccgg cggtggctat ctgcttctcg cgtccgagga ggtaaatatt
    70681 tttgttttct ttttccataa cagggtaaat tgctgaaatt gctacctttt ttgaaaaatc
    70741 ccccatcccg gttcaagaat gtaaaatata tctataaaaa ctgaagcaag gagaggagca
    70801 ggatgaggga taaacactaa cttaactatc acaacaaatg atactgttac atcactaatt
    70861 caagcttttc ttcgggtgca tcgcggtcaa tgcggtccat cagggcacca aggatttttt
    70921 caaggattct cattcctcca tggtagccta ctgttgggaa gtaactatgc cctacacggt
    70981 ctaatatcgg gaagccgtag cggacaaaag gtatatcttc gtccctggct atgtatttcc
    71041 cgtaggtgtt gcctatcagc aggtccactt tttcttgttt aatccattga tgtaaaagga
    71101 acatatctgc cgtttcgcca tttttgaatt tggcctcagg tactttttca ccgagtattt
    71161 ctttcatgcg gctttcaaaa agtttgcccg gggttccact gacgatataa acaggtttca
    71221 tatctaacat caccaaaaac tctgtcatag gtatcagcca gtcggggtcg ccgtacaagg
    71281 ccactcgttt tccatacaag tacgaatgca tgtcggctat catgtccagc agtcggccac
    71341 gttcgttggt caatgactga ggcactttta cgttggcata cctgctaagg gccgtgataa
    71401 agcggtcggt tgcttgtaag cctattggaa catcgcaaat gtcgtatttc actttgcatt
    71461 tgtattccag ctttttggcc gcttcttcag tggcccattg gcctatgcct aacgagaact
    71521 tggcatctcc tgcgctgata agctcttcaa tggttgttcc ccctttcggg taaaaggtgt
    71581 atttgccatt gagcggccca tcaaggattc ccgatgtatc gggaaacatc atggtgttgg
    71641 cttgcatttt tttggctatg cgtttgattt cgcgcatatc gcaaggctca atccagccgc
    71701 tgatgaggtt tacctggtag tttgaaatcc ctgtgtgttg cgcaaaatag gaggtcatac
    71761 ctaacaacat attggcatat cctgttacat gcgagcctac ataacttggg gtgttgcagt
    71821 gtatcacgtg tttgcctttt ggtactttgc cttcttcgcg tgcctttgtc aatatttggt
    71881 tgaggtcgtc gccaatggtt tcgctcagac aggttgagtg tacggctatt atttccgggt
    71941 cataggtcga aaatattgtt tctatggcct gcaagaggtt cgattgtccc ccgaacactg
    72001 aagatccttc cgtaaacgaa ctggtcgatg ccataactgg ctctttgtag tggcgtgtca
    72061 gcgtgctacg gtgatatgag caacagccct gtgagccgtg actatggggc aaacaccctt
    72121 gaatacctaa ggctgcatac attgccccta tgggttgaca ggtcttggcc gggtttattg
    72181 tcagcgcttg cctgtctatt actttatcgg ttgtatgtcg tagtaacata ttttttaatt
    72241 tttagatttg agaatcaaga atcaagagcc aagaatttta tcaattgcca agtcttgatt
    72301 cctggctctt ggctcttttt atctgttttt ataaaacatc tatcacaaag ttggcttcta
    72361 cttgtttatg cgtttcccag ggggcttgca ggtacttcca tacagtagct ccggtgagtt
    72421 tgtcaatgtc tttgtagaag tttatagctc cggcaaatcc tgcataaggc ccgccatagt
    72481 cataactgtg aagctgcttg agagggatac ccatcttttg taccacgtac ttttctttga
    72541 taccggcaca gaaaacatcg ggcttgtaaa actctatcag cttctcggtt tcccaatggt
    72601 tgacatcgtc aatgacgagg gcattctttt tcatacttgg catcatccct gagtaaccgc
    72661 ttatttcaag gcctttggct tcgagggctt tcagttgctc ttctgtttta cgtggcctgt
    72721 agcgggtttc gtctttttct acatgcaact cttcaatgtt gcggctgtca gcgtccactt
    72781 gtattgtagg tattaccttg cggccttcgt agtcatcgcg gtgcccaaat tcgtaccctg
    72841 ccgaaacggt ttccatttcc aattcattga aaagttcctg gtagtggtgg gcccgtgaac
    72901 ctcctacgaa gagcattgcc agcttgcctt tggtacgggg ctttattttt tctaaagcct
    72961 ctttcaccgg tggcatttct tcggctatca cttcttcgat tttgtctatc aaagctttgt
    73021 cttcaaagta ctggcctatt ttccgaagcg atttggctgt ggcttcagcc ccgataaagt
    73081 ttaccttgaa ccaggggatg ccaaacttgg tttccatcat ttcggccagg tagttgatag
    73141 aacggtggca catgaccacg ttgaggtcgg catggtgtgc ctgctcaaag gagtcgatgg
    73201 tagagttgcc gctaaaagtt gatattagcg ttattccgca tttttcgaga atcctttcta
    73261 tttcaaatgc atcacctcca atgttgtatt ctcccagcag gttgattttg tacttccctt
    73321 ctacaggcgt atcattgagc cctacaacat gcttgaaaac cccgttgttg gctatgtggt
    73381 gccctgcact ttgactaacc cctttataac cttcgcaact gaacccaaag atgttgatgc
    73441 caagttcttc cttcatttcg cgggctacgg catggacgtc gtccccaatc aagcctaccg
    73501 gacaggtaga gaatatacca atgttcttgg gcttgaatat ctcgaaagct tctctgatag
    73561 ctgccttcaa cttaatttcg cctccaaata caatgtttga gtcctgcatg tcagtcgaaa
    73621 agctgtacat catatagttt ggggcatcgg gtgaaggtgg tttggtctgg ttacgacggg
    73681 taagccaggc ataaaagctg catccgatag gcccatgcac taagttgatg atatcgcgtg
    73741 tagggcctaa cactacccct ttgcaacctg cataagtaca accgcgttgg gtgataatac
    73801 ccggtatggt acgcacgttg gcctgaatgg gctggtcttt ctccgggtca tttataatca
    73861 gctgtttctc acgtttttta gctatcttag aaggatactt gctgagtatc tcttttttta
    73921 gcgccgacgg atcgggcaat attttttctt ctactttcat ccgttaataa tttttaaata
    73981 aggtcggatg gaactcgcat gatgaatttt catccatgtt tgtgattagc gaatcatgct
    74041 cgtttcatat ttttcttcca ttagtcaagt acactatctc cactttctcc tgtgcggatt
    74101 tgaatggttt cggctgttgg cagtacgaat atttttccat cgccgctgtt gcctgtttga
    74161 tttgctttta ttattgtctg gatagccaaa tctgcctttt cgtctgttac cgtgagctgg
    74221 atcattcgtt tggttttcag acggtgctct tcgtgtacat agtcagggtt tttcttgttt
    74281 tcttcataag taggccctac acctaatccg cgaccccgtc cctggacttc tccagaaccc
    74341 atgaaagaag gcaaaccagc ttcggctaaa gcagcttttg ttgcgtttac cttcttcatc
    74401 cgtataatgg ctactattgt tttcatattt tttgaatttt aaagctctat ttttccgccg
    74461 ttgttaattg tactggggga aagggagtaa gagctgactt ttttaacaac taacttaatt
    74521 atgtcctttt ttcatgtccc gccattggag ggatttaggg aggcttttat agctcttttt
    74581 taccattact gattgtccag gcttctaaaa ccgggataat aaatattttt ccgtccccaa
    74641 attggccttt tggctgtgtg cgtgccgcat taagtattgt ttcgacaacg atagatttgt
    74701 cggcatcgtt taccactatc atgagcgatg tttttgcaag ttcatcatag gtgatgttgc
    74761 ctacttttaa cccgcgctgt tttccgcgcc cgtaaacctg cgattttgta acggcaggga
    74821 agccattttc taacaatgcc ttcattactt ctactgcctt ttcaggcctt attattgctc
    74881 gtatctgtaa catatttttt attttttctt gttaacaatt gaatcttatt atttaaatcc
    74941 ctgattttgc ttaacgttta gttgaggatg ccgtagttga taagcagttc ttctaactcc
    75001 tcgatagcca aaggttttgg tatgacaaac atcttgtttt catctatttt gcggcacaat
    75061 tgacggtatt catcagcttg tgggtgtgtt gggtcgtact caatcactgt tttacggttg
    75121 atttctgccc tttgtaccat attgtcacga ggtacaaaat gtatcatttg tgtgccaagc
    75181 cttctggcca attcttcaat catctgaaac tcattgtcca ccttacggct gttacaaata
    75241 aggcctccaa gttttacatc tccggcttcg gcaaacttta caataccttt gcagatgttg
    75301 ttagctgcat acatggccat catctcgccc gagcaaacaa tgtagatttc ttgtgcctta
    75361 ccttcgcgca ttggcattgc aaaacctccg caaactacgt ccccaagtac atcatagaat
    75421 acataatcta tttcttgcga ctcgtcataa gcgccaagct gttcaaggag gttgacagag
    75481 gtaatgatac cacgtccggc acatccaacc ccgggttcgg gaccacctga ttcaacacat
    75541 cgcactcctt tgaaccctga ttttaccaca tcatccagtt caacgtcttc gccttcttcc
    75601 cgaagggtgt ccaatactgt tttctgtgct aatccgccaa ggagtaagcg tgtagagtct
    75661 gcttttgggt cgcaccctac taccattact tttttgccca tctctactaa tccggctacg
    75721 gtattttggg tcgttgtaga ttttcctata ccgcctttcc cataaattgc tacttttctc
    75781 ataacattaa ttttttaaag tttcgtccat ctatggtatt acacaatgcg tgccaaacga
    75841 tgtaagtaac agttttacag catgttacaa aggtaatacg taagatttta ttttcttttt
    75901 atgccgtagg attacaattt tgtaggtaaa aaatggaaaa cttacattta tgttagaaaa
    75961 aataggtgga aaaatagttt atgctatatg agaaatatat tttaccccta atattggtga
    76021 agagtgtttg ttttgtataa ataaaaacgc cattgtccga tataaaatta atattagggc
    76081 taaagcccta ttacatcatg gttttgacta agaaggtgtg taaaaagtca aaactgtcca
    76141 aatttacccc taaatccctt gaaggggatt tttgacaatc aataatttat gataaagccc
    76201 tttaggggtt tgggggttaa aaagactttt tacacagcct cttgcaaata gttacttact
    76261 gaaggacttt agtcctaata cttttaccgg acaacagtga attaaaatca tatcatgacc
    76321 tgtataattt gaaataatct caggtagagg tagtattttt ttgtttgctc cggaggagca
    76381 acctgtttat agaaaaaata caatcagcga attaaaagcg cgcctaataa atattataaa
    76441 atgtagaaac caaatttggc gcgcaataga agaaactctt tataggttta gttttgttgc
    76501 gcaccataca gatgttgttc atttaaatat tattgtgctt ggtgcgctta tttttatttc
    76561 aacttgtatt ctataaacag gacactcgcc aagcgagtta aaatcatctt agcgcgtcca
    76621 atgttattaa gttaagctaa agacacttcg acaggtatag tgtccactat gaaataaaag
    76681 gttgttgaac ttgtcgaaac gcccttttat ttctgtccag ctccttaact taatagcatt
    76741 gaatgtgcgc caacggtact ttgcggtaaa aagataaatt tattttgaca cttttttaaa
    76801 acgctttggt tcaatattgt atttgtgcaa acgtaaacga agtatccgtt ctgttatacc
    76861 cagcttttgt gcagtttcaa aagtacatcc atgggtgctg atgagattgt ctataatcac
    76921 ctgtttttcg acattggcta caatatcttc cagcgtacct tccatttttg tttttgacga
    76981 ttcggctgtt tgaagcgttg gtggcaggtt gtttgacctt attacatcat cgatacttaa
    77041 tattgctgca cgttctatca cgttttcaag ttccctgata ttaccaggcc agtggtacat
    77101 catcaaaagg tcaatagcag aggtgctgat acgctttatt ttcagcccat ggcgtttgtt
    77161 ggccttggca ataaagtggt tgaccaatat ggggatatcg ttgcggcgtt cgcgcaacga
    77221 ggggatatat atcgggaaaa cgtttagcct gtaaaaaagg tcttccctga attcgcactt
    77281 tgtaatcatt tcttcgaggt tacggttggt agcagtgatg atacgaacgt ctattttcag
    77341 cgttttcgaa cctcctaacc tttcaaattc ccgttcctga aggatgcgca gtatctttac
    77401 ctgtgtaggc aaaggcaaat ccccaatttc atcaaggaag atagtgcctc cgtcggccag
    77461 ctcaaacctc ccttttctta acccgtctgc cccggtaaaa gcaccttttt cgtggccaaa
    77521 aagttcgctt tctatcaatg tatccggcaa ggcagagcag tttactttta tcagaggttt
    77581 gtcttttcgg gcactattgt aatggatagc attagcaatc agttctttgc caaccccgct
    77641 ttcgcccctg agcaatacgg ttgcattggt tttcgatact ttttgtatca gttcaaacac
    77701 cgattgcatg gcacttgagt tgcctatgat ttcggtgttt attatagctt ggtttaactg
    77761 gtcctgaagt ttcaggtttt cttgccttag cagggccaac tcgtaagcgt tttcctgtgt
    77821 agctatgata gagtgggcca gcatgctccc aataatattc agtatccgaa attccagttt
    77881 aaacgagata tcgttataat attctaaact aaaagcaaaa aatcctaata cctttcctgc
    77941 tgctttaatt gggacgcata tcatggatat ttcatagcta aaatccacca aatcgcgtga
    78001 gctttttata aatgtcggcg tatgcgacat ttttggcaaa agcattggtt cgcctgtttc
    78061 tatgatggtt ttgcttactt tttcaagttt tcccctaagg tttgaaactt cttcggattt
    78121 tagtccgtga tattcttcaa gtataacatg atgcaaatct gcgtttaata tagtcagcga
    78181 gcaacaagag attccgcaat aatcggctaa gtgctttaaa atgggcttta ttgattcttc
    78241 aatatgcttt actttctgca attctttact tatatcgtaa agaaaagaca actcagtata
    78301 ttttttttca cccgttttca tttataaaaa tataatcaaa aaagttaaaa aacttagatt
    78361 atgttttaaa aaatagtgat aagattaata aatgtaatct tattggcaaa tttaattaat
    78421 aaaatcaaat tcaatctcta ttttttcact tttttgattt tctttcagcc tgaatgtccc
    78481 ggtgtacgtt atagcaacat tttctttgtt tgaggcttca ataagtatgt tagtaagctt
    78541 ttcgcgagca acttcatcgc aatcggcatt gaaatggagc aacatccggt cagggtcttt
    78601 attgtcgaat tgtaaaataa ataccgaatt gtcggcaaaa accaaatcgt catagacata
    78661 agtaacatcc agtcctgtag atgccactat attcattgct tttcccaagg gcctgaatgg
    78721 tatttccatg acttttcaat ttgctaatga gtaatacaaa aacctgaacc aatttacttt
    78781 tccaaatagc cagtctgcct cttcctggct ttggcatttg gcatgaaaaa cgttggctgc
    78841 ttcttcaaat tcgtctatgg tgaagctcat ttggtgcatg tccaggattt ttaccagttc
    78901 gcctttgttt ttgcaacggt agatactttg gcgcaattcg tctctttcgt ccatctgtct
    78961 aaagagcact cctacattgt ttattgacat atctgtaata ttttagtggg tttaatgaca
    79021 atctgattcg gggccgcagc taaggcaatc tttggcacat tcgcctgtaa gcagcgattc
    79081 gtatatgtca aaggccctta tctgcccagc tttgcagcgg cttatgtttt cttctaacga
    79141 agtgctatgc gctttgcatg ttacaatatt gttttctttg aataccctaa gcgacataaa
    79201 agagtagaaa gggcttacaa catgcgatat tttgttgttt accagtgaat tgaacaacag
    79261 ttgagcttcc tggtgggtgt tttcaagctc aatggtctgt tgcctgtcat tggccaaatc
    79321 aattatgtaa atgagttttg aatgggcaaa gtcttgcgcc aattcgttgc tgttttttaa
    79381 tgggatacct attttcatac cataaagatt ttatatatgg cataacaaaa agcattccaa
    79441 agtgtgtaat gttcagtaaa tgaacaattt gaaacaactt atcctattta taattttgtt
    79501 aatcatctca aaaaattaca aaaaagtaaa tgttgagatt aatgaccttt ttaaagtttg
    79561 acataagaag aatgtatagg tatttgatat ttacaggtat tacaaacttt tgccttgaag
    79621 caaaagattc aataaaagcg gcggagcctt aaaaagaaaa attccttatt attgcctttt
    79681 tgttcgattt cggccgagaa gcctaacgct cttgttgttt gcatattgca acatcacaag
    79741 gcttcgaggg tgaaagagtt aaggcgataa gagtttttga gcaacttttt tcgataaaaa
    79801 gttgcatgag actttgtatt tattgctttt taaacaataa ttgacgatgt tgtccttata
    79861 tcgaactcgc gtttttttag gaaagacttt ttaatcctac tttgacagat tagcaataac
    79921 cttctcctaa ccgcgtagcg gtggaatatt tgcagaagat gagttttata atgattaagc
    79981 cacgtagtgg cgaaatatta ttttggcact acgcacctta tttcgatggt gtattctctt
    80041 ttattaaacc caactattcc ctcttaattc tcttttatta aattccagtt tattaaacct
    80101 gtcaaagtag aattaaattg ttgcctataa tgtaaatgca gtaagtggtt atatcattct
    80161 ttcgaaagga acccgttgat gttggcgtac aacaactccg ggtctattgg ttttgccaga
    80221 aaacagtcgc atccggcttc tttgcttttg gttatgtatg agtcaaaggc ataagccgac
    80281 tggataatca caggaaggtc tttttttacc tttttgataa tacgtgtagc ctgccagctg
    80341 ctcatgtcgg gcaactgaat gtccatcagg ataaggtcta tgtctttatt ttcgtgtgtg
    80401 gcgtatattg catcagaggc tgttcctact tgtattacct tagctcctgt tttgtcaagc
    80461 aggtctttta aatacatagc gctcacaggg tcatcttcga caatcaaaaa tgtacgggaa
    80521 ctataatttg ggctactcat tgggcaaatg gggttttatt tgggctatac aaccattatt
    80581 tctgcttctt tcttttgaag gacttcttct accttctttg agaagtggtc agttgttttt
    80641 tgcatgtctt cttcggcttt ttttatttca tcttccggaa tgccttgttt ctggagtttc
    80701 ttgatttctt caatagcttc acggcgcgca ttacggatac ttactttagc attttcgcct
    80761 tcatgcttta cttgttttac aagaccttga cggcgttctt ccgtaagcgg ggggatactt
    80821 atgcggataa cttcaccatt atttaccggg gtaaggccaa ggtttgcagc aaataccgct
    80881 ttttccaaag catctattaa cttcttttcc caaggttgga taactaaggt acgtgcgtcc
    80941 tgtgtattta cattggctac ctgggtaata ggcactctgg aaccatagta atctaccatt
    81001 actccggcaa gcatcgcagg actggccttg cctgccctga ttttaagcaa ttcgctttca
    81061 agatgcacaa gcgctttgtt cattttatct ttggcatcgt ccatgtaaag ttgtacttct
    81121 tcgctcataa tggtaaaaat ttaaacgacc taagttatta tttttttatt aatgtaccaa
    81181 ttttttcgcc tgaaattaac ttcttcaggt tccctaactt attcatatcg aaaacgataa
    81241 taggcatatt gttttcagaa cataaagtga aggcggttaa atccatgatg ttcaagcctt
    81301 tgctgtaagc ctcagcaaaa gtaagttcat catactttgt tgctttaggg tctttctcgg
    81361 ggtcagcggt ataaactccg tcaacccttg tgcctttaag caatgcgtca acttcaagtt
    81421 ctacagcacg caatgctgaa gcagaatctg tggtgaaata tgggtttccc gtacctgctg
    81481 tgataatgat aacgttgcca ttgttcatgg cttcaatagc tttacttttg ctataagctt
    81541 cacctacagg gtgcattgaa aaagcagtaa agagttttac ttttgttttc aggctttcaa
    81601 gcatcgactc aatggccaat ccgttgatag ttgtagcaag catgcccatg taatcgcctt
    81661 tgtagcggtc aaagcctttg tcaacgccac gcatacccct gaaaatgttg ccacctccga
    81721 taacgatggc tatctgtacg ccttcctttg cagtttcaga cacttgcttg caatattctt
    81781 ccagacgggc agtatcaatg ccgtgtcctt gttgtcccat cagtgcttct ccactgagtt
    81841 tgagcaatat acgcttgtac ttcaacataa ttatctgttt tctgtcgcca aaattagaaa
    81901 ttttgctgta aaacacgtaa aaaattgacg atttgaaaga taatttatgc gagtggatgg
    81961 aaattggaga taaaaaaagc agacgagtca atattcaatt agtgagacat cagttcaatc
    82021 tatacggttt agtttttcaa tgcttttccc cgctggcgca tagccgtcaa gctaagaaat
    82081 tttgtatatg aatttgattt tgaagccctg tagaggcgac atatttgtag aaaaaagttg
    82141 ttaaacagag aacgcaccaa gcacaaatat aatacaacga acgaatgctg aatggtgcgt
    82201 aatttggaga agctttttaa atgcagttct tctattgcgc gcccgaaaga gtttcgtaca
    82261 ttttcaaaca ttcatctggc gcgcttttta tcaatacttc ctgtctctac aaatatgtcg
    82321 cccctacagg gcttgaatac aatacttatt tcttagcttg gcggtggtag gggtggctat
    82381 atcatatttt ttaatttgtt taccttggtt cgtagtacct gtcattgtct taaaaaataa
    82441 cgatataggt atatatttac aaaattttca acatggcatg caacctttgc catgcgaata
    82501 taattattag tcaaaacccc tttattagaa cttttggacg aacgtattct tatatcaaaa
    82561 tgtatctcag gtaatgaaaa agcacaaaag cttttttatg acacattcgc taaccaaatg
    82621 tttaggttgt gtttgcgtta tactggcgat taccacgatg ctgaagatgt tatgattatt
    82681 gcttttcata aggtttttaa gaatattaga aattttgaat accgtggtga tggcagtttg
    82741 aaaagatggg ttaaaacaat tatgatcaat gaatcgctaa tgtttttgaa aagttcgcgt
    82801 aagtttatgt ttgaaactat tgataatact catattcaaa tagcagatgg aatagaaaac
    82861 actatagatg ctgaacaaat atatcaactt atccttaagt tgcccgatgg atacaggaca
    82921 gtggtaaact tgtttattat tgatggcttg agccacgagg aaatagctaa tcagcttggc
    82981 ataagcgtta atacttctaa gtcgcagctt tcacgtgcca gggctttgct gaaggaacaa
    83041 ctttttaaat tagaagaaaa tggaagataa ttttgataaa tatctgaaac agaaaatgga
    83101 tgctgttagt gatgctccaa tttccgagca tttgtggaac aaagaaaata cctggcagaa
    83161 aattgccttg ggaattaaac ctcaggggaa aagtgtgtct atcaggtggt tttatgctgc
    83221 tgcttctgtg ttgttagtgg ttttaattgg ttcattggtc ttttacaacc atctttcgaa
    83281 acaaatgagc cagcttaggg ctgaaaataa aaggcttggt gaaattgcaa ttaatcgaaa
    83341 caacacttca ggccctgttg aaaaggttct gattaagaca gacaccgtca ttaaagagct
    83401 tataaaaaaa gaaaatgttc aaaatacttt attcatccac gatacagtgg taattgtgaa
    83461 agcctctgaa gaacaattag ctgtgaatgc catgcctgac aaggatagtg tatcagcatc
    83521 atctaccaat gaaccgccaa aaaagagcaa acttcatttt gttataaaca acgaaatttt
    83581 tgtcgaaaat gagaaatcca aagtaaagct tattggttct aaggaactta cacaaaaaga
    83641 cccggagcga acaaaacaag atgcttctat caaattcgcc tttaactgat gttgtaactg
    83701 tgagatggta gcatttcccc tcctttatat ggaggagtgg gagcatagcc gtcaacttaa
    83761 ggaataattt agacaccatt ggaagcaaca agaaaatatc caccccctac ccccgccagc
    83821 gggggtaggg ggtggaatta aacgcaacta attttaagtt gatggctagt ggttctatta
    83881 ttaaaataac ctaaaaatgt aaaatatgaa aacattaaaa aaatctgcat tcattgcatt
    83941 atttgtattt atatctctta gtatcactgc acaaggtttt cccgaaaagc ttattctgct
    84001 tacaagtagt gatgtgaaaa tggttatcga gacgagagaa atattggaca ttccgcatga
    84061 aaagaaacag gatacccttt ttatgaacct gctttcggac attagcaaag ttcaggattc
    84121 gctgaccagg ctgaatgtgc ctttgcaaat attttacagc acaatggaca aggatagcag
    84181 aagcatgaaa gttgccaata acgctgagac taaggctgag ttcattttca aaggcgcttc
    84241 tgatacggtt aggagcaatt ttttcaatta caaaattgag tgtgacttga attggaacaa
    84301 caagctgatt ttatatacca acaacctgtc tgagctggga gaattgaaaa acacttcttt
    84361 ttctgatata gttcagcagg ctcaagctga cattgcttta cgcaaaatac cgtcttataa
    84421 agccaaaacc tgtttgtaca aaataaacca taaccaactt gacgctactt ctgctgacat
    84481 caaacaacaa ggtacattta ttattactcc tggcatggat tttggcttaa gttatctgaa
    84541 tgacaggttt atgcccaata tctccatgtc tatggcctat gtattttcgc ccaaaaatga
    84601 aaagaaatgt atgcttggta tttcgggaga aatattttat aatatggata ctgcagaagc
    84661 ttataaaatg caaggtaatt cttttgtaga tgttttcttt catgctaaaa acccttatag
    84721 tccgatttat tatgcgtctg attttcggtt ttttattggc tatttagttc atcgtgaagg
    84781 ggttgttttt cctaaaaatt catggcgttt tggcttggat atgccggcag ggaagaactt
    84841 tcgtataaat tatgttgtct atttaaatga tttcggtagt aataacaaaa acaagggttt
    84901 gtttgaaatt gggataaaat ataagttctt ttaaaaattg ttcaaaattg ttataatttg
    84961 cggtcggcaa cctttaacct cgataaatat gattaatcta cttatccatc actggaaaga
    85021 aaaacaacgc tctccatttt ggcaaaaaag cattgcacta aatatcattt tagggatttt
    85081 atgtttatat ctgttgctca atgttttagc attaagcttt tttgccgata agataattca
    85141 ggaaacatac aaaggcagag atgttgtaga aacctttact ggcttattgt tttattactt
    85201 ttcattcgac ctggttatcc ggtttttgtt tcaacagctt ccaacgttat ccattcagcc
    85261 ttacctcacg cttccgatta aaaaaagcaa actgttgcat tatcctttga taaaaagcgg
    85321 gtcaagtttc tttaatatag tggcaatact tattcttctt cctttcttta taaaggttgt
    85381 atgtgcaacg cactcatttc cattttgttt tgcatggatt gtagcgttgt tttcattcat
    85441 tattgctaat aactatctaa actttttgtt gaagaaatat tttgccaagc gtccgttact
    85501 tatcctgtta ttgctggcct ttgtgggctt gtttattttc tttgatattg ccaaaatcgt
    85561 ttcatgttcg agttactttt ctgcgatatt gatttacctg gcaaatactc cttttctgat
    85621 catcattccg gtggcgttgg ctgcacttac ttattatttg gcttattttc ttcttaagaa
    85681 gaactcctat gttgaagaca aactgactga taagcgaagg aatgctgata gttttttatt
    85741 cttaaaacgt tttggggaaa ttggagattt agtgcatatt gaaatcaaaa tgattttgcg
    85801 gaataagcgg cccaaatcat tgttatactt tagccttata tttttagcct atgggtttat
    85861 gttttataaa aagcagaatt tggacaatta cttacttctt agttttgtcg gggtactaat
    85921 gactgcaatg tttgcattga atcatgggca gttcatgttt gcttgggaaa gttctttttt
    85981 cgatagtttt atgacgaaaa aaatctcccc atacaattat gtaaaatcga agtatttgtt
    86041 ttttactgtt ttatgccttt tatcgtttat tgtttcttta ccttatgcct taataagcta
    86101 taaaataggc ataatcaatg tgtctctatt gctttataat gtgggtgttt catcatttat
    86161 tctgatattt tttggcacct acaattcaac tcgtattgat ttagggaaga gccagtttat
    86221 gaattatcag ggtatcgggg ctactcaatt tttattaata ctgccaatta tgggtatccc
    86281 tgtgctggtg tatttactgt tcaatctttt tgatattccc caatatagct tttatgtttt
    86341 agggatttta ggcattattg gcattgcttt taataaatac cttctacagc ttgtgactga
    86401 tcagtttata aaaaggaaat ataaaatggc ttatggtttt agacaaaaat agaatgttat
    86461 gttaaatatg attgaaataa atgaccttca gaaggtttac ggaaagaaag tggctttatc
    86521 cataaatagg ctcaacatca gggaaaagga atttgttggg ttagttggca acaatggagc
    86581 agggaaaacg accatgctaa gtttgatttt agatttaatt gagcctacaa aaggatttgt
    86641 aaaatcgaaa ggaatgatgg tgtcggagtc cgacgattgg aaaaattata ccggttcgta
    86701 tttgaacgaa ggctttttga ttccattttt aacgcctttt gagtttttag agttcattgg
    86761 taatttgcat ggaaaaaacc gtgcagaggt aaccaacttc ttaaaagaaa ataacagctt
    86821 ttttaccgaa gaggtttctt caaaaaaata taatcgggaa ctttctgccg gaaacaaaaa
    86881 taaacttggg atattagctt cgtttcttcc agaaccagag atacttatat tggacgaacc
    86941 attttctaat ttagatccaa gttcgcaatc atggctaaag tcaaagttga aaaagctcaa
    87001 cgaagaaggc gttacaatat tgatttcaag ccacgattta aaacatgtta cagagatatg
    87061 tagccggata ttgttgctcg agaacggaac tattataaaa gataccaata ctaatactga
    87121 gactttggtc gaattagaga attattttaa tgtttgacaa ataaaggtgg taaacaactg
    87181 tcatagctgt tacagttttt tgaaaattcc ttacgagaga aaaatatcag gatagaaaac
    87241 gaatgataag tttgtgctga gctgagtaaa taaacgcttt gaatcttttc atttttctac
    87301 aatttgatag agagctcaag ttgaccccct aatcctaatt gtacaatttt ctgcagagtt
    87361 gaaatcctaa cttctttgat attgttttca atctttgaaa tataagattt ggttgttcct
    87421 accttttctg ccaattcggc ttgagtgagt cctttttcaa gtctggcttc atgcaataaa
    87481 gctccaattt taaaattttc ataaccatca tcaagttcgt ccctagtttt agttcctttt
    87541 actccataat gcttatcttt gaattcatca agggtcattt tgctatttgt tttcattttc
    87601 gtattcctcc ttaattttaa gggctcgttc tatttctttt ttgggagtct tttgcgtttt
    87661 cttttgaaat ccatttgcaa gaattatcag tttcccatgg tcaaaaaagc aaaagattct
    87721 aaaaatgtca ttgccgagtt taatcctaat ttcatacaat ccttctgttg actcgatatg
    87781 ttttaaaaat atctctggaa ctctttctaa ttcttctatc agctcaattg tccaaataat
    87841 tttagctttt actctttcag attgtttgat aaagaaactt tcaaagtaat ctttgtagaa
    87901 tattgctgtc ctatattttc tttttgaatt caaatgacaa agttataaaa tgtttcactt
    87961 aggtgcaacc ttttcttttt tgttctaaaa aagcaactgg agggaataaa gattattcta
    88021 atactttatt aaaaattttt aggtaaaagt aaaatacaac ttcagattta taccggcaac
    88081 tccaaaatat acgtatcaaa atccagcaaa tcctcgaata gttcatcggg gctgactttg
    88141 taccagtcgg gataaagctg gcaaagctgg ttccaaaccc aggcattgcc tttgctgcaa
    88201 tacacttttt tcatcgactt gttgccaatc catccacccc agagttgatg aggaatttga
    88261 gccaacaatc ctccaacatc atagcgttta ccaaccagtg atttgctgag ctcaacgtat
    88321 ttggctgcat caaaacttaa tcgaggcctt tttactacaa catttactgt atgaacaatg
    88381 ctttgctcaa tagggcgaca tactacacgc gggaaaatag cctcatagca taggattccg
    88441 tcctctttca cttctaccaa tgaggaatgg ttgaattttg accgggtaaa tatttgttca
    88501 atgcggccta cagcaatggt tttttcatat agcaatgtat ctcctgtacg tagttcgctt
    88561 ctggttatct ttggtgcttt cataatttaa agtattgatt tatacttgtt aaagctatga
    88621 aaatttgtag atattccaaa gaaaaccaca ggatttgatt taggagaatt gctcagggct
    88681 ctatttcccc ttctttacaa ggagagcttg tcctgcctca ggcaggaggt aggggtggta
    88741 ttgttaaatc ttgtatgttt gatgctctgc ttttgctttg caagggctgt tttaattcaa
    88801 tttcagtatt atttcctcca cttccttcct catctttggt ttttgttgtc tttccttcag
    88861 gtcatctggc aataattcgt tattgcctgc atagcctaat gcaataacta tgattggttc
    88921 gtattcctga gaaagttgaa gtgcgttttt taaaagttca gcatcaaagc ctcccatggg
    88981 gtgagtaact agtccttggg ctgtagcttc aagcataagc tgtatgtttg ccatacccgt
    89041 gtcatgccag gcatatttgt ttttgacaat tgtcccatta cgttcgtatt ctacttgagc
    89101 aatggatgca ataagtaaag gagcatcttt tgcccaacgc ttgtttcctt ctgtcaaaga
    89161 attgtatatc ttttcgtaag ttacttctcc tttctttcca tacatatatc gccaaggctg
    89221 aacattattg gcagatggag caaaggatgc agctttaaac attttatcta taaagctttg
    89281 gtcaatatca atctctggat tgaaagaaac aacgctcttt cgcgattgaa tgatggtgaa
    89341 aagttccgac atattctatg tttttaattc ctttttgttt tagtaataaa gaatattgat
    89401 caacttttta gtctaatttt ttgatagttc caccccctgc cctcgccagc aggggaaagc
    89461 atttatcgta ttctttctaa attatatcca atgtacaatg aataaatgtt taattcaaag
    89521 aaatgtcgag aaatcctgtc ccccgctggc gggggtaggg ggtggacatt ctatcagaaa
    89581 cgcaattgta caatacaatc tctaaagatt ggtgagcatt catagtcatt gattatggaa
    89641 atgatacttc aataaattaa caaaataaat ttttttattg ttcaacaata aaattactta
    89701 gctgtttctt attacaaatc agcaatttga gaatattcaa aaaatattat atgctacgtg
    89761 ttaattgccc gacaaaaact ttttactttt gtgggtgcaa ttgtgaaaca tttcacaata
    89821 aattttgtta ataccaaaaa tccttttgta tgcctaccat agtcgtgttt ttgctgtatg
    89881 taatgggagc tgtttttctg gtagctttta tgttaggtgc atcacatgtg cttggcgaac
    89941 gtcacgaagc ccgttttcgt aatgaaccat tcgaatcggg gatgccgctt accggagatg
    90001 ccagggtgcg ttattctgct catttttaca tgattgccat gttttttgtt atttttgatc
    90061 ttgatgcagc ttttattatc acttatgctg tagcatataa agaacttgga tggcccggtt
    90121 atatcggaat tgctgtattt attggattac tgatagctgt attggcatac gaatggaaaa
    90181 taggggcact tgattttggg cctaacggca aaaagattat tggggctact cctaagaata
    90241 ccataacgga gaagagttta gaatagagta aagagcatag aataaagaac caagatatta
    90301 agataacttg attcttgacg cgaaattaaa aatataaaaa atgaaatttt ctctcaatcc
    90361 tgccgaacgc gttggtatgg gcgactaccc aaaattgtcg ctggagcagg ctgtgcagaa
    90421 gtcggtattg tttgcaaacc ttgatgacct tattggttgg ggtcgcaaat attctatttt
    90481 ccctttcggt tttggtcttt cttgttgctt tgttgagttt gcaactgcat ggacaagccg
    90541 tttcgatatt gcacgctttg gagccgaagt actacgtggc tcacctcgtg aagccgacct
    90601 gatgattgtg gctggcactc cctttatcaa aatggtacct actattaaaa ggttgtacga
    90661 ccagttgatg gagcctaaat gggtgatatc aatgggttca tgtgccaatt ctggtggaat
    90721 gtacgatata tatagtgttg ttcagggtat tgataaaatt atgccggtgg atttatatgt
    90781 tcccggttgt cctccccgcc ctgaggcctt cttacatggt cttttgttgt tgaaagagtc
    90841 tatagggaag gaacaacgtc cgtacagctg gacagttggg cctcaggatg tttataaagc
    90901 tcaaaagcct tcgcttcgtg acctgaacaa tgagcgccgc atggccgttg acaagcttaa
    90961 acctgccgac gaaatttaat tcttgtaaag taagttttgt atcatgataa ttgatgaact
    91021 taaagcccgg tttggcgcag acaacataag agaacaaaaa acatccgatg ggatgcctac
    91081 agcatgggtc aatgcttctg atattcttcg gttgcttgac tttgttaaaa atggcatttc
    91141 aaaaccatat aaaatgcttt acgaccttac agtcgttgat gaacgtcatc gtcaatacag
    91201 ggattcaatg cctgctgctg acttttctgt ggtttatcat gttacttcat acggtcgtaa
    91261 tgaagatttg cgtattaagg tagcattaca cggcgaatat ccttcgcttc caagcattac
    91321 cggactttgg gctaatgcca actggtacga gcgcgaagct tacgatatgt ttggggttaa
    91381 gtttgaaggc catcctaatc ttcgtcgtat attaatgtct caatcatgga agggacaccc
    91441 tttgcgtaag gaatatcctg caagggctac tgaaataggg ccatttagca tgtcggaaga
    91501 tgaacgtact gctgctgaag aagaattggt atttaaacct gaggactggg gcttgaaacg
    91561 caagagcgaa gatgctgaat ttatgtttct caaccttggg cctgaccatc cggggacaca
    91621 cggcttgctg cgtcttattt tgcaattgga tggcgaagag attgtagata ttgttcctga
    91681 cattggttac caccatcgtg gtgctgaaaa aatggccgaa cgccaaacat ggcacaaata
    91741 tatcccttat actgaccgta tcgactatac tgccggtgtt ttgaacaaca tggcctatct
    91801 gatgtctgtt gaaaaacttg atggcattga ggttccggca cgtgctcaaa tcatcagagt
    91861 aatgactggc gaaatgttcc gtatcatcag ccatttattg tggtatggca cttttgcaca
    91921 ggacttaggc caaatatccc cggtattcta tactttcaat gaccgtgaac gtgtttttga
    91981 tatcatatct gctattaccg gagggcgtat gcatgctaac tggttccgta ttggtggtgt
    92041 ggctcaagac ctccctaaag gctggcagca gatggtgctt gattttgtaa aatatttccc
    92101 taaaagtatt gacgaatggg ataaggtaat tatgagcaac cgcatcatca aaggtcgaac
    92161 acagggtata ggggtattta atactgacga ggctatagaa tggggtatta caggtccttc
    92221 acttcgtgca acaggctttg gttgggattt gcgaaagcaa cgcccttaca gtggttacga
    92281 aaactttaag tttgatgtac ctgtaggtaa aaatggcgat tgctacgacc gtgccagggt
    92341 tcgtattgaa gaaatgcgcc aaagtttaag cattattgaa caatgtgtta agttgatgcc
    92401 agatgggcca tacaaatcaa acagtccgct taccactcct cctacaaaag aaaacactac
    92461 aaaggatata gaaactctta ttcatcactt ccttaatgtt agctggggcc ctgttattcc
    92521 tgccggcgaa tctatggtgc aggtggaagg gactaagggc agcaatggct attatctggt
    92581 gagtgataat aatacagcgg cgtaccgttg tagggtacgt accccgagct tcccaagtat
    92641 ccagatggtc ccgtatattg caaaaggcta tactattgca gacttgctgg ctattttagg
    92701 ggctgttgac tttgtattgg ccgatttaga tagataacac ggctccccaa aggggaagag
    92761 atgggctata aaaaatgata gtgaatatta acaaaaaata aaaaaattac cttaaactcc
    92821 cctcctttgg aggggttggg ggaggctttt atatgttaag cgaaaaagaa atacacgaaa
    92881 ttgaacacga aattgggtta taccctgtta aaagtgctgc ttgtattgag gcattaaaga
    92941 tagtgcagga gcatcgcggt tgggtatctg atgaaagtgt taaagatatt gctgcaatac
    93001 tggagatgac tcctgaagat gttgatggtg ttgctacttt ctacaatatg attttcagaa
    93061 agcctgttgg acgtaatatt atttttgttt gcaattctgt tacctgttat atgatggggt
    93121 atgagagcat ttatcagtat atgtccaaaa aattaggcat tacctgggga caaacaactt
    93181 ctgatggccg ttttactttg cttcccaatc cttgtcttgg cgattgcgac catgcacctg
    93241 ccatgatgat taataaagat cattacaaca ggattaaacc tgagatgatt gattcgatac
    93301 ttgaaaaata ccaataagta gatattcagc ttattatgac aacaaagccg cttacacaaa
    93361 atataaaacc tgacgggaca gtttgttcgc tcgaggaata cctgaaaaat ggcgggtacg
    93421 aaggagctcg caaagctgtt aaagaaatgg aaggcgctgc tgttatccag gctattaaag
    93481 actcgaattt gcttggacgt ggaggagcag ggtttaatac cggcctgaaa tggtcttttg
    93541 ttcgtcagga tgatccgaat gaacagcgct acattattgc taatggagat gaaatggagc
    93601 caggtacctt taaagacagg ttattacttg aaggcgatcc gcatcagctt attgaaggga
    93661 tgattattgc tgctcgtgcc atcaatgcta ctaaggggtt tattttcgtt agatgggcat
    93721 ataaaaaagc ccataaactg atacaagaag ctcttgatga tgcatacaag gcaggattgc
    93781 ttggcaaaaa cattatgggg agttcattta gctttgattt atacttgcat accagtgtgg
    93841 gaagatatat ttgtggcgaa gaaactgcac ttatcaattc attggaaggc aaacgtgcca
    93901 atccacgctc aaaaccacct tttccacagg tgagtggctt atttggcaag cctactattg
    93961 ttaataatat tgaaacttat tgcaatcttc cacatatcat caaatatggt tcaatctggt
    94021 tcaaggaatt gagcaaatct ggcgaaggcg gaacaaagct ttatggcgta agtggcaggg
    94081 ttaagaatcc cgggttatgg gagttgcctt taggtactac cgttcgtgag cttttggaag
    94141 ttcatgctgg cgggatgctt gatggatata aattccgtgg attattaccc ggaggtgctt
    94201 caactgactt tttggttgag gagcatctgg acactccaat ggacttttca tctgttgcaa
    94261 aggttggaag ccgtatgggt acaggaacaa tgattgtcat ggacgacaaa acctgccctg
    94321 ttgcttttgt gcacaacctt attcgctttt tctccaacga atcatgtgga tggtgtactc
    94381 cttgtcgtga cggacttccc tgggtggaaa agattttgac aaagattgag gccggagaag
    94441 gcgaagaaaa agatattgac attttgaaat ttcatacaaa atacctggcc cctggaaata
    94501 ctttctgtgc tttggcccct ggcgctatgg aaccattgca gagtgctttg aagtattttg
    94561 gcgaggattt tgaaaggcat gtgaaggaaa aacgctgctt ttataagtaa tatttgattt
    94621 agcgaattat tatatatggc aacaataatc gtagatggca aacaatatca ggctgaagca
    94681 ggtgataatc tgctgaaagc ttgcataaag ctgggtattg atatacccta tttctgctat
    94741 catccggcgc ttggttctgt tggttcatgc aggctttgtg ctgttaaacg tttccgcgat
    94801 gcagatgata agcgtggacg tattgtcatg agctgtatgg aacctgtttc tgaggggatg
    94861 attatatctg tcaatgatga agaggttaaa aacttcaggg catctgtaat tgaaggcctt
    94921 atgacaaacc atcctcacga ctgtcctgtt tgcgacgaag gtggcgaatg tcatttgcag
    94981 gatatgactg tcatgaccgg gcataataac cgcaggtttg agtttaaaaa gcgtacacaa
    95041 aagaatcaaa atcttggccc ttttatccat cacgaaatga accgttgcat tcagtgttat
    95101 cgctgtgtaa gatattaccg cgactatgct ggtggtaagg attttggtgt ttttggttcg
    95161 cacaaccatc tatatttcgg gcgttttgaa gatggacaac tcgaaagtga atttagtgga
    95221 aaccttgttg aggtttgtcc tacaggcgtc tttactgata aaactttaaa gaaacatttt
    95281 acccgcaaat gggatttgac aaatgcccca agcttatgta cccattgtgg tgtcggatgc
    95341 aatactattg ttagcgaacg ttatggtact gtccgccggg tgatgaaccg ctacaatcat
    95401 catgtgaatg gatattttct ttgtgacaaa ggccgttttg gctatgaatt tgtaaatgac
    95461 ccttccagaa taactgtttc ttcttataaa ggaaaagctt tagatgttga atctgcatta
    95521 ttaaaggcta ctgaattgat tcgcagcgga aaaaccattg gagtgggttc tcctgttgca
    95581 tctttagaag ctaattttgc tttacagcag ttggtagggg ctgagaattt ctatgctggg
    95641 gtttcaaaag ctcaatggga attagtttgt gctgtccgcg atatctatcg ttcaaagaat
    95701 gtaaaaacgt tatctcttgc cgaagttgaa aaagccgagg gtgttttaat ccttggagaa
    95761 aatattatta atactgcgcc acgtgcagct ttgtcggtta ggcaggctgt taagaataaa
    95821 ccttttgctg gtgctgctgc cctaaatata gccgattgga ataattcggc tgttaaaatt
    95881 gctgttggaa ctgaaaaagg cccgctgtac atttttgctt cgggcaaaaa caaattagaa
    95941 gacgtagctt ctatttctaa agatattgat acggcttatg ttgaaaaaat tgctgaagct
    96001 attgctgcat cattaaaagg aaatttgaat actgtttcat taaatgaaga tgaaaaagct
    96061 actgtcaaac aaataactga caacctttta tcttgtaaga aagtagccgt catttccgga
    96121 acttcttcaa tgagcaagaa gcttctggcc ttgtcagctg aaatagcttc cttattgaat
    96181 acctcttcat gcgaaagcgg catttatctg gtagttcctc atgccaattc attaggattg
    96241 gctgtattaa caagtaatac tattgaacaa gttgctgatg cagctgaaaa tgttgtttta
    96301 ctggaagcca atagcataga agaaagtgtt attacttttt ctgaaaaggc aaagaatgta
    96361 attgttttag tttataagtc acctgaaaaa ctcaacagca aagcgcttgt gttagcatct
    96421 gctacatttg ccgagtctga agggacattg gtaaataacg aagggcgtat gcaacgttat
    96481 ttcagggtaa aaaatactgg agaagacgtt aaagcttcat ggatgtggct tagccagcta
    96541 aaggctatgc tcgataacag taattatttg tctttcgact ctcttgttaa aaagattgga
    96601 gaaagctatc ctgaattgaa caaaatagtt gatgtagccc cgggagctgg ttatcgtgag
    96661 tcggcgatga aaattcctcg tcaaacaccc cgctttaccg gacgtacttc aatgtatgcc
    96721 aatgtagatt tgcatgagca aaagcctcag aacgatgaaa actctgctct tgcctttact
    96781 atggaaggaa gcagagaggt tcatcctgcc cagctcaatg ctatctattg gaaaccgggc
    96841 tggaattctg tacaggccac caatcattat ctgcgcgatt tggataatat attgaaggct
    96901 cagcagatgg ggattaatat ttggaacgta gaaaagtagt taatatatta taatatggct
    96961 ataggattca taatagttgg tgttttgtta ttggtagtta ccattgctgc cgcaaacatt
    97021 tatatagagc gtaggttgct ttctctgtgg caggacagac gagggcctaa ccgtgctggc
    97081 ccttttgggt tccttcaggt acctgccgat atgattaaaa tctttctgaa agaggactgg
    97141 attccgcctt ttgcagacaa agcagttttc gttttggccc caactattgt gatgctcagc
    97201 attcttttta gttttgcgct cgtcccgttt gctcctggtt tccaggtcgt tgattccaat
    97261 atagcattat tgtttttcct tgctaattct tcgttggctg tttacagtat tgtgctttcg
    97321 ggttgggcct ccaataacaa atatgccatg ataggggcaa tgcgtggtac ggctcaaatg
    97381 atttcgtacg aagtgtttat ggggctttcg ctgatgggtg tggtgatgat ttccggaggg
    97441 acttttagct tacgcgaaat tgttgaagca caacgtggca tgtggaatgt agtgcctcag
    97501 tttgttggtt tttgcatatt tatgattgct gctgtagctg agtcgcaccg ggccccgttc
    97561 gatttacctg agtctgaagg cgaattggtg gctggttttc ataccgaata ttctggtatc
    97621 aagtttggta tgtttttcgt tggcgaatat cttggaataa cgctcatatc agcaatgata
    97681 gtgaccctat tctttggagg ttggttaggc cctgcttttc tacctccttt cttttggttt
    97741 atgttgaaaa catttgcatt tatagctttc attatactcc tcagggctgc tttaccgcgt
    97801 cctcgttatg accaattttt aggtgttggc tggaaaatat tgcttccact gactttacta
    97861 aatgttttga ttactgcagg tatattgtta gcaagaggct aagtttaaat agatagaagg
    97921 aaatgttaag tatattaaga agcatttgga ttacgtttac tcacgctttt cgcaaacgcg
    97981 aaactcgtga ttatcctgaa aaggctccgt ttttgccggc acgctatcgt gcccgtatta
    98041 tccttacaag ggatccggaa ggaaaggagc gttgtgttgc ctgcaacctt tgtgctgttg
    98101 cctgtccaac tgattgtatt tcattgcagg ctactgaaga tgaaacaggc cgcagatacc
    98161 cggaattttt tagaataaat ttctctcgtt gtattttttg tggctattgc gaagatgcct
    98221 gtcctacaaa tgctattcag cttactccgg attttgaaat ggcagaatat tacaggaaaa
    98281 atctggttta tgaaaaggaa aaccttttga ttagcgggcc tggtaaatat cacgattata
    98341 acttctataa aaatgctggt attgaaatag gaggaaagaa aaaaggagag ggacttaatg
    98401 aagaacctcc aattgacgtt cgtgatttaa tgaattaata agaaatattg aagatatgag
    98461 tatattattt ctgatatcgg ctgttgttgc tgtgttctct acagcaatgg taattacacg
    98521 ccacaacgct gttcatgctc tcatttacat ggttatttcg tttattgcag cttctgtcat
    98581 gttctatact ttaggggctc cttatgctgc agtgctcgaa atcattgtgt atgccggggc
    98641 tatcatggtc ttttttgtat ttgtggtgat gatgctgaac atgagcgtca gtcctgaaga
    98701 tgaaaaaagg gctacaggaa tcaagtcatg gattttccct atcatactta cacttgtgct
    98761 aatagctgat tttgtttatg cctttataca aggcactccg caaacaaaca tggtaaaggt
    98821 cattgatgcc aagcgtgtgg gagtttcgat gtttactact tatttattga tggccgaatt
    98881 ggctggtata ttgctgttag tcgccattgt cggggcatac catttgggta gggctccaaa
    98941 acgcaatatt cacagatatt tacaaaacga acaaactcaa aagtaacaat atatgatacc
    99001 attagagtgg acattgctgg ttgcaggaat tttatttgct ttaggcctga cagggctttt
    99061 ggtgcgcaga aaccttctgt ttatgctgat gagcctcgaa ataatgacta atgctgctgc
    99121 attggctttt attgctgctg gttcgcattg gatgcaacct gacggccagg tgatgtttat
    99181 atttatcctt actgttgctg ctgccgaaat atcgattgct ttagcattgg ttttgctgat
    99241 gtttaaacat aacaaaaccg ttgataccag taaacttgat agtttagaat aagaatactt
    99301 aagaaaataa acatagtatg ttacaattta tttggcttat ccctttattt cctttgcttg
    99361 gctttttatt gctggcttta ggaggcaaat attattcgaa aaagcttgtt gctattattg
    99421 gaagtgcatc ggtgggtatt tctgctttgt tgacttttgt tattggcctg ccaatgctgc
    99481 aaaatatcga aaacatgcct tctgttacca atgttcttta tacctggata gatgttggtt
    99541 cgtttagtgt accattttca ttcaagattg atgctttatc cctcgttttt gtctttgtaa
    99601 ttacttttgt tggttttttg attcatatat attccactga atatatggct caagaagagg
    99661 gttatgcccg tttcttcgct tatatgaacc tgttcgtagc ttcaatgctt atccttgtta
    99721 tggcagatag ccttttgctt atgtatcttg gatgggaagg ggttggtctt tgctcttacc
    99781 tgctaattgg ctttttctat cgcgatccgg ccaacaatta tgctgcgcgt aaagctttta
    99841 ttgttaccag ggtaggagat gctgccatgt taattggttt gttgatgctg gtttttgcat
    99901 ttaacacact tgatatggct caaatatcag atcttgcaaa aaatagtgtt tctatttcac
    99961 ctgcatttgc tacgatagtg gctttactgt tattaggtgg cgcagttggc aaatcggcac
   100021 agttgccatt acaagtttgg ttgcctgatg ctatggctgg tccatcacct gttagtgcat
   100081 taatccacgc tgccactatg gttacagccg gggtgtattt gattgcccgt atgcatgtta
   100141 ttttcatgct ttcgcctttt gccatgcttg tggttgcttc aattggtgct ataacattat
   100201 taattgccgg tgttagtgct ttatatcaaa cagatttaaa acgagtatta gcatattcaa
   100261 ctatcagcca aataggttat atgtttttgg ccttgggtgt tggagcttgg agtgcggctg
   100321 tttttcactt tatgattcac ggtttcttca aagcattact attccttggt gctggggctg
   100381 taatactttc gctgcatcat cagcaggata tgtttaaaat gggtggactt tacaagaaga
   100441 tgcctgttat tttctggact ttcctcatcg gttcggcttc tttggctgcg tttcctttta
   100501 tatcagccgg attctttagt aaagatgcta ttatttggta tgctctttct tcaccacaag
   100561 gaggttgggt tttctggctt gctgctatga taggggctat tattacttgt ttatatactt
   100621 tcagaatgat atatgttact tttttcggta aagaaaaaac tcatgttcat tatactcccg
   100681 gaattgcaat gactttacca ttaattgttc tcgctattct ttctacatta gcaggttaca
   100741 tcgagttacc tcataatatg gggcatattc agtggttttc atcatttgta acgaattatt
   100801 tgcccggtat tactgtttca gaacatttca atgaatgggt atttcagatt attgctatgg
   100861 ttctttctct tggtgctttg gcaattgctt tcaaatatta caatgagaaa tatgtggaaa
   100921 gctttaccat gaccccggta aaactctttt tccaaaatgg gtggaacttc gataaactct
   100981 atgatacact ttttgtgaaa ccttttgttt ggcttgcaaa tgcaaacaaa gaggatgcta
   101041 ctgacaaaag cattgataaa ggagctattc aagttagtaa gcttagcaat cgcctttctg
   101101 tcttacaaaa tgggcgcgta ggttggtatc ttgcaggaat ggccattggt atcatactgg
   101161 tggtttcgtt aatgttgttt ataaagatgt aaattttgat ttcaaaataa atatactatg
   101221 gcattagttg gcattttggc tgttttaata cttggggctt tgatttcatt gcctgtaagg
   101281 cgaattcaca agcaattacc ctggtatgtt ggaattgtag ctgtccttac tgcaattatc
   101341 atgtcttttt cccttgggaa tgcatcttct ggaactgaaa aatggtttgc ccggtttact
   101401 tcgtcatgga tacctgcaat aggcagtaat tttagccttg caatagatgg ccttagctat
   101461 attatgatat tattgacttt ggtgatgggg cttgttgcta ttggtttttc tgaaaggaga
   101521 gaaaatagca gctcttttat cttcaacagt ttgtttatgt tagccggggt tgttggcatt
   101581 ttcatggcca ccgatttgtt cttgtttttc tttttctggg aaatgatgct cttgccattg
   101641 tattttctta ttatctctgc aaacaaagag ggtagcgaca aggctgcatt taagttcctt
   101701 atttataccc agggaagcgg gctcatcatg ctgatttcgt tgctggcatt gtatttcatt
   101761 catggcaatc aaactgcaat ttattctttt gcttttgagg acctgctcaa tacccaaatg
   101821 tcaataacaa cttctatgct tgttatgtct ggttttttgt tggcttttct tgtaaaattg
   101881 ccagtttggc ctttacatgg atggttacct tctgcgttta aaacagctcc tgcaattgct
   101941 atattttctg gcttattgat aaaaaccggg gcttatggta tcatgcgttt tgctgtacct
   102001 ttatttccgg aaggttcatt aattatttct ccattaatga tgattcttgg tgttgtcacc
   102061 attttatatg cttcatttat tgctttttcg caaaatgata ttcgcgtaat atctgcttat
   102121 agcggaatca gccacatggg ctttatattg attggtattt atgctttcaa cgatttgtca
   102181 tggcaagggg ttggcgttca aatgattgcc agttcagtag gtactgcatc gcttattttg
   102241 ctgggcgaaa ttatacatcg aagagctggt acttatgata ttgcccattt aggaggtatc
   102301 tggacttcaa accctaacat tgccggtatt ggtactttct ttgccatggc tgctttagga
   102361 ttaccaggca tggcaaactt cattgctgag tttttgattc ttgcaggtgc ttttcaaata
   102421 agtgttttgt caacagtgct tgcttctatt ggcattgttg ctgcggctgc ctattcattg
   102481 cgcattattc aaaaggtttt tattggtgag gaaaagattt gtattagtat tattgatgtt
   102541 acattaaggg aaaaagctgc agttgctgta atgatggttc ttttgttatg gataggcttt
   102601 tatccttcga tggtaacaag ccgtttagaa ccttttataa cgcgtacact taatttttca
   102661 ccacctgaaa ctcccgggac tgaacaggga aatgtggatg aagcccaggt taaatatagt
   102721 tttcaagtga ttaaaaaata aatataccga ttatgtttgt tgctttagct ccaatgtata
   102781 ttatcgctgc tgccattgtt gttttgatgt tgctggcagc atttgtccga aaccacaagc
   102841 ttttggccat tgtgtctgct ttgggcttta ttgctgcttt tggaactgtt tttagccatt
   102901 gttcctgttt gtcaaaattt gcctgggagt tatttgttgt tgatgattat tcttgggcat
   102961 taacagcagt tactcttctt tctgctttgg caattaatat tttcagttat ttttattttg
   103021 aaaactttga tgaacaaaag gaagaatatt atgtgctttt gcataccgct gttttaggaa
   103081 cattggtgat gattgctgcc aagcattttg ttacattctt tattgggctt gaacttttga
   103141 gcattccaac ttatgtcatg gtggcttatg tccgaagcaa gaaggaatca gttgaagctt
   103201 caattaaata ccttgctact gctgctgctt catcggcggc tttgcttttt ggcctggcat
   103261 taatttatgc ggctgccgga actatggaca tctacactgt aggaaggttt ttttatatag
   103321 ctcaatctgt tccggcttat acttatgttg gaatagctct ttttattgtt gctgttggtt
   103381 ttaaacttgc cttagctcct tttcacatgt ggtcacctgc tgtttatcaa ggctcagcgg
   103441 ctcctgttac tgcttttgtg gcttctgtgg caaagctcgg tgttattgct tttgttttaa
   103501 ggcttttcaa tgagtccaat ttgttgaata tcggagcatt aaacatttct ttagtggtat
   103561 tggctgtatt atctatgttt attggcaact tgtttgcagt tggacaaaaa aacataaagc
   103621 gcatgttggc atattcttct gtggctcaca tgggatacct tatggttgct ttattagcag
   103681 ggggtatggt tgccaaaagt gctacaggct tctatattac ttcatatttt gttgctacat
   103741 tggctttatt tggtgctatc agtgttattt ctaaaaatga cgaacctaaa tctattgatt
   103801 ctcttaaagg tttatattat agaaacccaa tagttgctgt tgttattact attggtttgt
   103861 tagctttaat cgggatgcca ttaacaggtg gttttatggc aaaagttttt gttgtaaaag
   103921 ctggggccgg tattcagcaa tggtttctgg tttggatgtt gattattaac agcattatta
   103981 gtatatatta ctataccaaa tatatcaatg tattgttttc tcatacctct gaagaggttg
   104041 taaaagaaaa agtaccatat ttattggcaa caatactcat tatcattgta gtgttagtta
   104101 tttggatggg tgttgcccct cagttgtggc tcaatttgtt ctataattga gaaaaaacta
   104161 ttgacacaat caaaaccata taacattacc atttatactg atggggcttc gagtgggaat
   104221 cctggtcccg gagggtacgg aactatctta atgtccgggc ctcatgaaaa agttttgtcg
   104281 ggcggttttc ggcttacaac aaacaaccgt atggaactcc ttgctgttat tgttggtctt
   104341 gaacacttaa aatttgaagg ctgtaacgtt actatttatt ctgactcccg atatgttgtt
   104401 gatgccgttg aaagagggtg gttgtttgat tgggaaaaga aaggcttcaa agacaaaaag
   104461 aatcccgact tatggaaacg ttttcttttg ctttaccgta aacacaaaat aaagtttatt
   104521 tggattaaag gccatgccga caatccatat aaccagcgtt gtgattggct tgcggttaat
   104581 gcttcaaaat ctgacgattt gatggttgac catggatatg aagaaggatt aaagaattga
   104641 acatattttt tttcaaatat ttgtcctaat tcacagaaaa ttactatttt ggttgtcgta
   104701 atgtaaaaaa ccatagatgc ccaagcaaga agtaatattg cattatcagg ggatatttaa
   104761 ctttgaaatc atcggacact tgctgaataa tctcaaggat gagtctgagg cgcgtaatat
   104821 ttctatttcg cactataaaa aaatattgtc tgttatgatt gaggcgctgg agaatgtatt
   104881 caagtattgt gaaaattttg aaaaagagcc aggacttttt cctcaatatt accctttttt
   104941 ttctttggaa tttaagaatg ggggatatgt tgttactact gccaatccta ttttaaaatc
   105001 tgacattgaa aaattaactt cccatattga taaaatcaac tcattagata aagagggctt
   105061 acgccagtta ttccgggcaa cattaacaaa cggtcagttt tctgcaaagg gaggggcagg
   105121 tttgggtttt attgaaatgg ctaagacttc ttgcgaaaag atagaatata cttttgtccc
   105181 tattaatgaa aggttctctt attatacatg tgttgtgaat atttctcaaa atgctttaaa
   105241 tgttgagtga tgaataattt ttaccttgct gaaacagagt ttacgcctga aattagtttc
   105301 aatatcgaaa aaagaaaatt agcttttaaa ggcgtttcgc gtcctgaaga tgttgtaaag
   105361 ttttaccaac ctgctgttaa ttggcttcgc gaatttgatg agaacgtact ttcgcattcc
   105421 agtgcaaagt taaatatccc atccatacat gttgaatttc gtttgacgta tttcaactct
   105481 gcatcatcaa agatgctgtt gcagttgttg gaaattatca aaaaagttca ggacaaagga
   105541 attgatatta tcattgattg gtattatgat gctaatgacg aagctatgta tgatgatgga
   105601 gctgatttgt ctgaagctgt aaacctgccg tttaattttc atactattta gttagaagac
   105661 tataagcata aaaaaacctt caacatttcg aaggtttttt actatttctt taaattcagc
   105721 atttatcggt tgtattgttt ggcataatat ttctcataat cgccagaaac aacattattc
   105781 aaccattgct cattagctaa gtaccaatct acagtttttt ccaaaccttc ttcaaattgc
   105841 aaagaaggtt cccatccaag ttcacgttta atctttgtgg catcaatggc gtaacgcaaa
   105901 tcatggccag cacggtctgt gacataagtg attaacttcg cagatgtgcc aggcgcccgg
   105961 cctaattttt tgtccattat ttcgcaaagc agtttaatca atttaatgtt tgtccactca
   106021 ttatggccac caatattata agtttctcct attacccctt tgtgaaatat tacatcaata
   106081 gccctggcat gatcaactac atataaccag tcacgaacgt tttctccttt tccataaatt
   106141 gggataggtt tattattctt gatattgttg atggaaagtg ggattaactt ttcgggaaaa
   106201 tggttaggcc cgtagttgtt tgagcaattt gaaatattga ttgggagttt ataagtatga
   106261 taatatgcac ggactaaatg gtctgagctt gctttagatg cagaatatgg gctgcgggga
   106321 tcataaggcg tttcttcggt aaacatacca gtttctccta atgaaccata gacctcatct
   106381 gttgatatat ggtagaattt tttgccctcc atattacctt tccaagcatc acgagcggcg
   106441 ttaagcaatg tcaaggtgcc aacaacattg gtctggatga actccagagg gttggtgata
   106501 gacctgtcca catgcgattc ggccgctaaa tgaattaccc cgtctggttg gtatttagca
   106561 aatatttcgc caatgcgttt ggcatctgta atatccgctt tttcaaaagt gtagtttgga
   106621 agctggtcta tatccctcag gttttcaaga ttaccagcat aagtaagctt atcaaggttg
   106681 ataattttgt aatcaggata tttggtaaca aataaacgta ccacatgaga cccaataaat
   106741 ccggctcctc cggtaataat gattgttctt ttcataataa tataagtatt taacactacg
   106801 acactaagtc acaaagaatt gtttcatagt gcctttgtgt tttagaattt attctaatga
   106861 caaaagatat tgaccgtatt ctgtttttga catactttct ccaagctttt taagctgttt
   106921 gatatcaatc cagccattac gaaaagcaat ttcttcaaga caggctacat aaagaccctg
   106981 tcttttttgt atagcttcaa caaaaccagc tgcttcaagc atactatcgg cattgccggt
   107041 gtccagccat gcaaaccctc gtccaaattt ctgcacctta aggttgcctt cttcaagata
   107101 tatcctgttt aaatcagtaa tttcatattc gccacgggca gaaggtttca aagccttagc
   107161 tttttcaact actttgttat cgtaaaagta taatccggga acagcaaaat ttgacttagg
   107221 cacttgtggt ttttcttcca gcgaaatggc ttttcctgtt ttgtcaaatt ccacaactcc
   107281 ataagcccgg gggtctttta cctcgtaacc aaaaacaatg gcgccgtttt ttagcgctga
   107341 agcttcgtgc agcatagcac tgaaacctgc tccataaaaa atattatctc ctagtatcag
   107401 gcaggaagaa tcattcccta catggtcagc tccaaggaca aatgcttgag caagtccgtt
   107461 tggcacttcc tgaattttgt aagtaaactt catgccaatg tcttcccctg aacccagtaa
   107521 ttgctcaaac ataggcaaat cacgaggtgt ggagatgatt agtacttcac ggataccagc
   107581 cagcattaac gttgacaagg ggtagtatat catcggtttg tcataaaccg gcatcatctg
   107641 ttttgaaatg g
//
LOCUS       DAOSPF010000002        93635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_230_length_93635_cov_7.434537, whole genome shotgun sequence.
ACCESSION   DAOSPF010000002 DAOSPF010000000
VERSION     DAOSPF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 93635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 93635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_93635_cov_7.434537"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..894)
                     /locus_tag="PK252_00545"
     CDS             complement(58..894)
                     /locus_tag="PK252_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT13225.1"
                     /translation="MEAKDKTLLVLWDFSPKCEFAYAHAVNVSKITGNSITLLHIIKN
                     EKEASSVQEQLEAVASKLEKTYFIKPLIKILVGSIFKTISSYASEGNTEMVIMATHGI
                     KGIQKFTGSWALKVIRGSKVPFLVVQDMPASQKLDNIIFPIDFKKENKEKIKWAHYLC
                     NLYKARIHIVHPLVTDRIFRGRIYSNLVFAKKYFDNTDIRFEIKTVGNKVNFAKDTIE
                     YAQQAQGNLILIMTSRTLTFMDYMLGPSEQLIIANSAKIPVMVVNPKPKSLSGGFSAT
                     GN"
     gene            1039..4647
                     /locus_tag="PK252_00550"
     CDS             1039..4647
                     /locus_tag="PK252_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT13226.1"
                     /translation="MLFCAVTSAQQPENNIIQGSPFIQNFTFPERSFSSGKISVFESP
                     DGGFVLGGRNKIILFYGNEFISMPLNGQINVIGDDKTSFYTGFNTVGVIRIFKNSLPK
                     LIPLVDDKKNHKNRFGQINKVFINDNVLVFNNRKYLYQSVDGSNIQIIDSSKVELRLF
                     KVNNALLVTKYEKGICQFTQGIIDNIPGGELFKNKDIECILSFCGALLVKIKGEKDFC
                     VLKNGQVAPVQFGFEDFVQKNGFSDAVILPSGDIAIATKSSGLLIFNKKTKSIVKISV
                     EDGLPENSLINLYVDKTGNLWLMHNKGISRIELNIPVSAYGFNAGLSGNVNDILKFKN
                     RLYVATQTGLYYAGFSGNNNKKHFDNIRFKHIEDISGEVNCLFVEKGNLLFTTPNGIF
                     KLNDDKVKTIFFDEIKSFERIQFQNESFFCIAKESSLDVFKYQNEQIDTVVIINMADN
                     PPSLVNEDNGIVWVKTENNDLLYLEKDKLGSIVSASDFVQIKTFSGNNFGVIKTESGL
                     RFTLAEKVFRYDKFRKIFVEEKITLFGVEEIPRPVKMITDVHNNQWYNLSGSLQGLKG
                     ILLYQKIGRDSVNTVFFNTGLDVNKIFVDTTSVWIAASDKLLRYDLQRNYKPQRQFSA
                     IIKRVIIGQDSILPVELEDPEIKYSLSNVRFIVSSTCFEGEPYIRYQHRILGKSKEWS
                     AWDSKSIITSGKLSPGDYTFQVRAISIEGRVSEITELHFTVLSPFYKTIPAYVVYVVL
                     LMFLIFVALRYKTWRFIKYKEGIERIVQERTKDILKEKEKSEILIANLLPKGTADELK
                     QTGKATSQKFNMVTVLFSDIQGFTKIAEQMNPDVLIDQLDAFFFHFDSVVEKYNIEKI
                     KTIGDAYMCAGGIPDKNITNPVEVVLAALEMQEYMAGLKKNNSDIWDLRIGIHTGSVI
                     AGVVGHKKLSYDIWGDTVNTASRMESSGEAGKVNISGQTYELVKDFFICEYRGKMPVK
                     YKGEIDMYFVKSIRPELSIDMHIQPNKKFFIMLQLLRLQDVQDEVIEKLINGLPKNLY
                     FHNVERVKELFNLVDLYSRAEEMSDEDKLVVKTAALLADVGYIQSYDEHEEYSIEFAK
                     DFLPRFKYNQEQIEKICALIDITKQNRKPQNKLEEILLDAEMNYLSRADFVSLNEQLF
                     KEQLEHGKIESKEDWVKMQVVLLSNHKYYTQVANVLRDVNPDSQIENLLQNLQKNT"
     gene            4765..5781
                     /locus_tag="PK252_00555"
                     /pseudo
     CDS             4765..5781
                     /locus_tag="PK252_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
     gene            5904..6779
                     /locus_tag="PK252_00560"
     CDS             5904..6779
                     /locus_tag="PK252_00560"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT13227.1"
                     /translation="MSGNPNKDSYRRLRSSYVTSTISLTLVLLLLGIVGLLILNAKKL
                     SDVVKENINFTIVLKPNVEEADILKLQKQIDLLQYVKSTRYLTKEKAAEEFSKDLGED
                     FVSFLGFNPLLSSIEVKMHAQYANEQYVKLFENKLKSYPVVREVIYEKSLIHLVNENV
                     RKISLVILTFSALLFLIAVALINNTVRLLVHSRRFTIRTMQLVGATKSFIRKPFLFKS
                     MVLGFMASTISIVAISGILIYTERNFSDMSLLVDFRLLYYLFGGLIVLGIMINLISTF
                     FALNKYLNIQKNNLY"
     gene            6789..7049
                     /locus_tag="PK252_00565"
     CDS             6789..7049
                     /locus_tag="PK252_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOT13228.1"
                     /translation="MAKAINKKESEAKVDFAFGKINYILMVVGVVIIFIGYLLMMGGK
                     SEDPNVFNADEIFSFRRVTLAPIVVLLGFAFEIYAIMKKPVE"
     gene            complement(7061..7591)
                     /locus_tag="PK252_00570"
     CDS             complement(7061..7591)
                     /locus_tag="PK252_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOT13229.1"
                     /translation="MKKLFILIAVALPLYGLAQQKAAIDSTKVMWLSLEQAETLCKTN
                     PKKILIDAYTDWCGWCKKMDKETFQHPEIAKYLNKYFYPVKFNAETKDTITFLGSKYI
                     NFGSRTHPLALSLLGWRPSYPTIVYMTERFEYLGPIPGYKNAMQMETILHFIAEEKYK
                     FTSLEEFEKNYKGKIQ"
     gene            complement(7653..9101)
                     /gene="cls"
                     /locus_tag="PK252_00575"
     CDS             complement(7653..9101)
                     /gene="cls"
                     /locus_tag="PK252_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOT13230.1"
                     /translation="MLRFLAENYLIILYSITVSFIIYLVIKQRGEPLKTISWLLVIIL
                     IPIAGIILYFYFGQNYRRQKIFNRKGLSDFERMRDFSHIQMQHLDKHAALLSEKLQSK
                     TQIMKLLLNNSKAMLTENNQLHIYTSGRDAFDIIISDITNAKSHIHLEYYIIEEDKIG
                     NQIRDLLVSKAKEGVEVRLIYDDLGSWGLGKKFLQPLIAAGVEVHAFLPVRFPYFTNK
                     INYRNHRKIIVVDGNIGHVGGMNIADRYIEGVAELGSWKDMQLRIEGEAAHSLQTIFL
                     IDWYFVTNQAINDDRFFPPCQINEKKIVQITASGPDSDWASIMQAYFSAIVTATDHIY
                     IATPYFIPNESILTALKTAALSGIDVRIMLPYKSDSHVVYRSTLSFVADLLEAGVKIY
                     FYAKGFNHSKLMLVDGVVASIGTANMDIRSFDQNFEVNAIIYDEELTNRLEAHYLKDL
                     QSCVVYSIEEWSNRPWINAAKESLARLLSPLF"
     gene            complement(9104..9979)
                     /locus_tag="PK252_00580"
     CDS             complement(9104..9979)
                     /locus_tag="PK252_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT13231.1"
                     /translation="MKKILIPTDFSDISKNSILYGFQLAKDLGLSVELLHVLEIYKFA
                     AGASEAELISSILPSDNIKMMEESAMTSFENLIQEINPSLPASVPFNKKVIAGHLVNE
                     VISESATEEIELLILAVSRSQDLITRFTHNTISSIISESMCPVMVVPSNASYKPIEKL
                     VFATDFNKADIALLQSFLKLSKNKQPDIQILHVSPKPIDFNAELKMTGFKQKIAESIN
                     CKCLSYKQIQAKPVVKGILDALDADNADILLMLKEHEGFFKSLLEIDATEKIAHYIKI
                     PMLSYREQLFNPNKK"
     gene            complement(10060..10428)
                     /locus_tag="PK252_00585"
     CDS             complement(10060..10428)
                     /locus_tag="PK252_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOT13232.1"
                     /translation="MKGLYSILLLVTSNAFMTMAWYGHLKFKEMKWFSSLSLLAVILI
                     SWGIALFEYFFQVPANRIGYKENGGPFTLVELKVIQEVITLTVFIIFTTLLFRNETFR
                     LNHLIGFIFLILAVYFIFKK"
     gene            10531..12063
                     /gene="gltX"
                     /locus_tag="PK252_00590"
     CDS             10531..12063
                     /gene="gltX"
                     /locus_tag="PK252_00590"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT13233.1"
                     /translation="MEGKKVRVRFAPSPTGPLHIGGVRTALYNYLFAKKYDGDFLLRI
                     EDTDSNRFVPGAEEYIVDSLRWCGIEFTEGVGIGGPHAPYRQSERKDIYLKYALQLVE
                     SGNAYYAFDTSEELDNLRKSQEAKGMAFTYNYEVRTRLKTSLNPEYADYQERIKKGEP
                     WVIRFKMPVNQNVHMNDLIRGEVIVNTSALDDKVLFKSADGLPTYHLANVTDDYLMRI
                     THVIRGEEWLPSLPLHVLLYKSFGWESKMPLFAHLPLLLKPDGKGKLSKRDGDRLGFP
                     VFPKHWDDPATKELVKGYDDYGFLPQAFINFLALLGWNPGTEQEIFNINELADAFSLD
                     KVSKAGAKFDFEKAKWFNHQYIQKLTNKQLAEMICSVLDEKGIKCEMSYLADICGLVK
                     ERMFFLNDFWANAYFFFQSPEGYDAEAVKKRWKDNVPELLKDVKAVLSDISHFEANSI
                     NDTLHHHVEAKQISMGVLMNALRICMVGASFGPDLPLIMEVVGKEETLRRIDKALNEI
                     KL"
     gene            12073..12711
                     /locus_tag="PK252_00595"
     CDS             12073..12711
                     /locus_tag="PK252_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13234.1"
                     /translation="MVRFLYSAIFVFVFSCAVAQECQIVASGGMAYRLFVIPDSLSAA
                     EAKHQKDLKPGNSFGFDVIFMYEQTGWGIGYSTFRGRKSLYNEEGSSLTALPSYDQVV
                     VNFFSLNYYLRKSVFSEKLFLNGKIGLGYVAYKDYCREGGEEYTKTSGTYGISSYIGL
                     SYMVSKHFLVGANLGLFISNTDKIKVDGVEQALSSKLRLTRVESNVCLGYIF"
     gene            complement(12754..13665)
                     /gene="cysK"
                     /locus_tag="PK252_00600"
     CDS             complement(12754..13665)
                     /gene="cysK"
                     /locus_tag="PK252_00600"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT13235.1"
                     /translation="MKAKSILETIGNTPHVRVNSLYGKNHEVWVKVEKTNPGSSIKDR
                     IALAMVEDAEKKGILKPESVIIEPTSGNTGIGLALVAAVKKYKLILVMPESFSIERRR
                     LMAVYGAQFELTPREKGMRGAIEKAKELVSSTPNAWMPDQFSNEANIEAHKRTTAQEI
                     INDFPEGIDYLITGVGTGGHITGVSETLKAKFPNLKVFAVEPDASPVISGGQPSPHPL
                     QGIGAGFIPQNLHTNILDGVIRITKDEAYEYARRAALEEGLFVGVSSGASLAAVAKKL
                     PEIPKGSRVLTFSYDTGERYLSIEGLY"
     gene            13902..14342
                     /locus_tag="PK252_00605"
     CDS             13902..14342
                     /locus_tag="PK252_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOT13236.1"
                     /translation="MNKIIYIFSLLAFLAVSCTSPDIEVVGITDISVSELSFTQVKGK
                     VGIEIDSKSMLSYTIDSSDLELWFDNNKLGKITLLEPFEIKGNTSKTYNVPFILKPEN
                     LKAAVTVIPKVLKGDQPKVAIKGSVQAKTLFLKKKIKFESLISK"
     gene            14374..14829
                     /locus_tag="PK252_00610"
     CDS             14374..14829
                     /locus_tag="PK252_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13237.1"
                     /translation="MAKFKLFITCVLCFLITHFSFSQSPTSDIFGIVTSKAGNEGTIK
                     ILQSNQIKTLVNAYVQSKQSDGLIVGYRIRIFSNSGQLARGKAASERSRFISLYPDIN
                     TYQEYEAPNFKIYVGDFRNKLEAFKAYQKISKDFKSAFIISTRINLPKL"
     gene            14846..16738
                     /locus_tag="PK252_00615"
     CDS             14846..16738
                     /locus_tag="PK252_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT13238.1"
                     /translation="MSELIKHECGIALVRLLKPLEYYQFKYGTWRYGLQKLYLLMEKQ
                     HNRGQDGAGVVSLKLDQKPGTDYLHRQRSNEPQAIEAIFRDIYSQYSNIEDRLLSDAV
                     WAKQNLPFAGEVFLGHLRYGTFGGSNIEFVHPVIRENNWRTRNLALAGNFNLTNVDEI
                     FNHLIELGQHPKGYSDTVTILENVGHFLDMEVQRKFILFKTQGFENKEISDLIAEHID
                     VAAVLRESSKRWDGGYAIGGIIGHGDCFVARDPYGIRPAFYYADDEIVVVASERTVIQ
                     TVMNAEYEKVHEIDPGKALIVKKSGFVSIEEIRPSMPRKACSFERIYFSRGSDKDIYN
                     ERKKLGELLTPAILNSVNGDFENTVFSYIPNTAESAFYGMAKGIEDHIKQYKKTKILE
                     LGPKLTPEALDKIMALRPRIEKVAHKDIKLRTFITQDKGRDDLVGHVYDITYGTIKKG
                     IDNLVIIDDSIVRGTTLKQSIIRILDRLGPKRIVIVSSSPQIRYPDCYGIDMAKLGDF
                     IAFKAAIALLKDSGKEHIINEVYKKAKLQQGLPKEEIVNHVKSIYEPFTDEQISDKIA
                     ELLTSSDIKAEIKIVYQTVENLHRACPNHVGDWYFTGDYPTPGGNKVVNTSFINFIEG
                     KNQRAY"
     gene            16728..18617
                     /locus_tag="PK252_00620"
     CDS             16728..18617
                     /locus_tag="PK252_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOT13239.1"
                     /translation="MHTNMQRFVEKLEARGELIRIKEFVDPILEIAEFTDRISKMPGG
                     GKAILFENTGTAFPVLTNAMGSDKRICEALHTKSLDEIGHRIDKLFHIFSSPKNTIWQ
                     KLSLLPQLKEVSNWMPRKKRGRGVCQQAVMQEPDLSKLPILKCWPGDGGRFVTLPLVN
                     TIDPDSGIRNIGMYRMQVFAKDKTGMHWHMHKTGARHYEAYKKAGEKMPVTVVLGGDP
                     AIIYAATAPLPDNIDEYILAGFLQNKPVELVKCLTNELWVPSEADIIIEGYVDPKEEK
                     LWEGPFGDHTGFYSEPDWYPAFHVTCITHRRDAIYPATIVGVPPQEDAYIAKATERIF
                     LSPMRFALAPEVEDIYLPAEGVAHNLAIVKINKTYPGQAFKVMNALWGAGQMMFNKTM
                     IVTDGNVDIFNPISVLEACAENVFQTHHICFQEGPADILDHAGKAFGFSGKMCIDATR
                     KMPQEIIAPFDNNVYSEKEAEIFIDQINNIENICSANSLKTKNGKTLVFAGFKKETPG
                     DVRTFISKLKNSTYLPSLLGIILFDKNTVLGHFSYLSWLTLSNIDATRDCFVVEHQRG
                     ITLIIDATSKSLEKDNFNRQWPAIIMSSDETIKNIDKKWSILTKLPFVHSPTLLSKKQ
                     SGIQE"
     gene            18686..21973
                     /locus_tag="PK252_00625"
     CDS             18686..21973
                     /locus_tag="PK252_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT13240.1"
                     /translation="MRVVFAILTILLFFENTSFSANRVLGSRTEMYTIENGLSQSYVN
                     HLFIDSRGFLWISTYDGLNRFDGLNFVVYKHNPVDTNSLSDNYVRHVCEDRDGNIWVA
                     TNNGLSCYLFSKGVFVNYYNDPQYASSISSNSIFNVFCDKNDVIWAKTLTSLEKFDKK
                     QQKFYHYYHHNDIFNQIIDDRNFDIFEDSREKLWIATKDGLSCFDKRLELFEHFTSTP
                     SDIKTISDNRIRSICEDCFGNIWVGTANGLNKFDIKQRRFYRIYAGSDNVSLISNKIS
                     SLYTADKKKIFVGTETGLSILNINSMTFANYPFFNIQNSEVPLSNIKSLLLDKANNLW
                     IGCREGLIKVDLKPLKFGLFRSSGGAFSENISSIYAQSKNDIWLGTWNNGLLRVNLGA
                     EEHSSSVKPAKRYFSNTSIYSLYKDKNNKLWISTENGLTIFNIKTNTFEPFKPKNKMP
                     YGFSNEKIYCFYGDGDKIWMGTKMGLLLFDNKFNEAHLFSKIPDSTGIQVNSAYCLTK
                     DKSGALWIGTNDGLICFNPETGEYKKYLSSPHYTQNALCSNTIYSLCFTSKGELWIGT
                     NAGLCKYNEEKDNFIIYSEVDGLPNNTIYAILEDQNNNLWLSSNKGIILLELPLGSFT
                     SFDQADGLQSFEFNHAAAYKDNTGCLYFGGINGLNYFYPDSLKPNKHIPSVQITLVEI
                     IGNSIRRISALSDGKLISIPANTKLFTINFASLDFTFPSNNQYMYKLSSKKSEGMWIN
                     NGTKNFVQYSNLPEGRYYFRVKGSNSDKVWSINEYSLILQVEAPWYKRKIAYFFYFLL
                     SALLVYGTVQLRTRKLIKTNKELIDKEKIAQKIARQKEELTLKNKSITDSINYAKRIQ
                     EAMMPSDKYLKRILPNSFLFHKPKDIVSGDFAWVSERDGKVLVAAVDCTGHGVPGAFM
                     SILGIELFRNIPNLSWKSPGAILKQINDEFVHIFSDMEDVSLRDGMDVALCIIDKKNY
                     TVHFAGAVNPLYLVTGDKLEEIKGSRFSISIEKRTEEIFFEDHMVQLKKGDMIYIFSD
                     GYADQFGGYEGKKFKFRRFRHLLLNISQLPLDSQKEYLLESIENWRGAYEQVDDLLVI
                     GIRADFE"
     gene            complement(22188..23912)
                     /locus_tag="PK252_00630"
     CDS             complement(22188..23912)
                     /locus_tag="PK252_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT13241.1"
                     /translation="MSFSQWIFEKLSIGTQGVTNKQGRYSIVITNLFTFLSLLLLFSF
                     IFISVSYENYFHAIIVAIVTIMVAANFIILQKTKNYIISNHFLMILTSVVYIYFVISG
                     GVGDYGYLWIFSYPIISMFLYGTRKGVLFAMGFLLIITLILVIQPAGLLKVHYTFPLI
                     VRIIMIYFFVSVLTYAYLYLTEQSFTVAETNIKEAKDESRSKDEFLSKLSHQIRTPLN
                     NIMVLGDLLSETKLDDNQRDLLESVLASTHNLVNVVNSIVKVSAAEITEKENKINFEL
                     LSTIQSSLKLFEQQYQQVVAFELEGKDKRYNVMGDPIRIKQIFLNLIENILKFRKPEG
                     NHVKINLELVKENSSQVEFNFQINFDFLLPVVNLTEESWLFSDDMHNKLSIVEPGFDL
                     SISKKLIEVLGSRLVASCSNGITTIEFSIKYKKSASEAPTAAPTIVSAVSSTVKNVDL
                     KEANVLLVEDNLINQKIVLLSLKTLVANIDVANNGKEALDMFGNKKYDIVLMDIQMPV
                     MDGLTATRKIRELEASTNSHVPIIAITANALSGDREICLSAGMNEYISKPFQLDVLVS
                     KMKELLKN"
     gene            24092..24937
                     /locus_tag="PK252_00635"
     CDS             24092..24937
                     /locus_tag="PK252_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13242.1"
                     /translation="MKKLFFVLIIALATLTSFSPDYVPTADEVNKFYQTKTLVVLDDN
                     PLAEYNFVIKDFMQKEWKLTQFDFIKQSEFEEKRMDKQYSFILLNSVRFDKDKSLARY
                     IFLSLVLGGDDKIVSNMPDLCPVPIAYYGVDEDHYNYKFQTILRFMQQHVELIHKNPK
                     LIKTNALKIYNDSMQSVKTKTLYLIKDELAPECNTEAKIKKIYPYKFKIVSREEVAKA
                     IDERNPDVVFLHKVGPEGTRLIARCYKILIGAADSKFYYFDYHKIEDDAPDGFLAKDF
                     KKLAK"
     gene            complement(25001..25852)
                     /locus_tag="PK252_00640"
     CDS             complement(25001..25852)
                     /locus_tag="PK252_00640"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOT13243.1"
                     /translation="MNYNPLTPEEERIILYKGTEAPFSGKYYNFDKKGTYHCKRCNAE
                     LYKSSDKFDSHCGWPSFDDEIQGAIKRVPDADGKRTEIVCSNCGAHLGHVFEGEGFTP
                     KNTRHCVNSLSIQFLPLENVDSIETTYFAGGCFWGVEYYMQKAEGIISTDVGFMGGKT
                     RNPSYKDVCTHTTGHAEVVKVVFNNKKTSFEQLARLFFEIHDPTQMNRQGPDIGDQYR
                     SEIFYTNEKQKEISLKLIQMLKAKGVDVVTGLTPATEFYIAEDYHQDYYNHKGTLPYC
                     HGYQKRF"
     gene            26051..27730
                     /gene="ettA"
                     /locus_tag="PK252_00645"
     CDS             26051..27730
                     /gene="ettA"
                     /locus_tag="PK252_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOT13244.1"
                     /translation="MSDDKKIIFSMVGVGKTFPPHKRVLNNIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGVEKAYEGEVVFSPGYTVGYLEQEPKLDDSKTVKEIVQEGVQKVVD
                     LLKEYEEVNNKFADPDLSADDMDKLIARQGELTDLIEQHDAWNLDNKLEVAMDALRCP
                     DADTNVKNLSGGERRRVALCRLLLQEPDILLLDEPTNHLDAESVEWLEQHLQQYKGTV
                     IAITHDRYFLDNVAGWILELDRGEGIPYQGNYTSWLEQKSKRLEQEEKQESKRRKTLE
                     HELEWVRMSPKARQAKGKARLNAYEKLMSEDTKQKEERLEIFIPNGPRLGNNVIEAKN
                     VSKAYGDKLLFEDLNFNLPPAGIVGVIGPNGAGKTTLFRLIMGHDKPDSGSFSVGETV
                     KVAYIDQSHTAIDPEKTVFEVISGGLDFITMGNKQVNARAYVARFNFSGADQEKKCGV
                     LSGGERNRLHLALTLKEEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHDRW
                     FLDRVATHILAFEGDSQVYFFEGSFSEYEENKKKRLGDTGPHRIKYKKLTR"
     gene            27901..28146
                     /locus_tag="PK252_00650"
                     /pseudo
     CDS             27901..28146
                     /locus_tag="PK252_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            28188..29558
                     /gene="hisS"
                     /locus_tag="PK252_00655"
     CDS             28188..29558
                     /gene="hisS"
                     /locus_tag="PK252_00655"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT13245.1"
                     /translation="MSMKPSIPQGTRDFGPVEMARRNYIYNTIRNVFELYGFQQIETP
                     AMENLSTLLGKYGEEGDKLLFRILNSGDYLTKVDDKDFRKDNSLGLSGKICERGLRYD
                     LTVPFARFVVQHREELTFPFRRYQIQPVWRADRPQKGRYREFYQCDADIVGSDSLLNE
                     WELVQIVNTVFEKLGINICLKINNRKVLSGIAEAIGAESHITDITVAIDKLDKIGIEK
                     VKDELKERGITESAVEKLIPIIEFKGSSRDKLELLRKTLAGSATGLKGIEEVETLLNY
                     IDGAQLSCEIEFDLSLARGLNYYTGAIFEVKAKDVAIGSISGGGRYDNLTGVFGMPGV
                     SGVGISFGADRIYDVLLQLNLFPSETSQTTRIMFVNFGKNEENYCLPLAAQLRQNGIS
                     CEIYPDAQKLKKQFDYANKKNIQFVVIAGEDEIKADEITVKNMITGEQTKISKEGIVS
                     FICKNI"
     gene            complement(29598..30788)
                     /locus_tag="PK252_00660"
     CDS             complement(29598..30788)
                     /locus_tag="PK252_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT13246.1"
                     /translation="MEETLLNSLSKEELIEKIMDLENQVVQLNKELVYHEEAQFEWSG
                     NLGKWHWNVKDNKVHFNSKKVEALGYSVQEIPDKIGFEFFTSKIHPDDYEMVMQNMRN
                     HLYGVSEAYECEYRIKSKSGEWKWFYDRGIIVKRDANNKPAELVGIVFDISAQKKIQN
                     ELESKNAQLLQLNENKSLMLSIIAHDLRGPLAALVSLINTISGTTVLAEKTALLNEFA
                     SSINNLYSLVNNLLEWAAFQKGIFGLQCQELSIFDVSQVAVNHLKLIADKKNIQIINN
                     IKPKAKSTFDEKMILTVFRNLISNAIKYTPQSGRIELFDKTSDGMIEITIQDNGIGIE
                     EEKLKDIFSVSSTKSTKGTEGEKGTGLGLVLCKDFVEKNGGSIYVKSQAGKGSSFIFT
                     LPVK"
     gene            30945..31169
                     /locus_tag="PK252_00665"
     CDS             30945..31169
                     /locus_tag="PK252_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13247.1"
                     /translation="MERLINLHIEKLPEGYYLATSDSIQGLIAQGRTISETIEIAKDV
                     AKKLIEAHEDQISQLAKVKDSFDYPLVIGV"
     gene            31171..31365
                     /locus_tag="PK252_00670"
     CDS             31171..31365
                     /locus_tag="PK252_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOT13248.1"
                     /translation="MGRLSGFRYRDIIQILKKFGFEFYRQAAGSHELWHNPVTKRFTT
                     IPNHPGDMPEGTLSAILKRH"
     gene            31416..31829
                     /locus_tag="PK252_00675"
     CDS             31416..31829
                     /locus_tag="PK252_00675"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOT13249.1"
                     /translation="MKKAAKKDEFDDTGLEVIGIATSLKDVTLLWQLNNTLHFKFKYF
                     GAIALDDKNTDAFPYFIDNESRQFQYALFYNRYKAYTLVPAFKQIDFFLVSYVKLYEE
                     EKAVLLKDLKSISGVSIAMHLSEPKKNAFEIFYIL"
     gene            32010..32153
                     /locus_tag="PK252_00680"
     CDS             32010..32153
                     /locus_tag="PK252_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13250.1"
                     /translation="MKRELSKNQKPEESFAFSNQKRSSFNHVYQASINSFDEDYFFEV
                     EDF"
     gene            32398..33924
                     /gene="purH"
                     /locus_tag="PK252_00685"
     CDS             32398..33924
                     /gene="purH"
                     /locus_tag="PK252_00685"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT13251.1"
                     /translation="MTGLKKIKSALISVYHKNHLDEIVKKLHAQGVKLYSTGGTEDFI
                     KQLNIPVTAVESLTGYPSILGGRVKTLHPKVFGGILARRNEKGDLQQLAEYEIPEIDL
                     VIVDLYPFEATVASGAEHQAIIEKIDIGGISLIRAAAKNYADVLIVASQAQYKDLLDI
                     LNDKDGQSTLSDRKMFAAEAFNISSHYDTAIFNYFNKEYNSNVLKVSIQESMPLRYGE
                     NPHQTGVFFGDFNKMFEQICGKEISYNNLLDIDAAVNLINEFSEPTFAILKHNNACGV
                     ASRSNMLDAWKDALAGDPVSAFGGIIITNRPITKEVAEGINTIFFEVVIAPAYDAAAL
                     EVLQLKKNRIILIIKETTLPTKQIRTVLNGVLMQDKDLKSENESDMRTVTQKAPTAQE
                     ISDLEFACKVCKHTKSNTIVLAKNKQLLASGVGQTSRVDALKQAIVKAGSFGFDLKGA
                     VMASDAFFPFADCVEIASQAGITAIIQPGGSVRDKDSIDYCDNNNLAMVFTGIRHFKH
                     "
     gene            34190..35212
                     /locus_tag="PK252_00690"
     CDS             34190..35212
                     /locus_tag="PK252_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT13252.1"
                     /translation="MGVFSFLTQEIAMDLGTANTIIIQNDKIVVDEPSIVAIDTKTGK
                     LIAIGETARQMHGKTHENIKTIRPLKDGVIADFNAAEQMIRGMIKLINKKNQLFNPAL
                     KMVVSIPLGSTDVEMRAVRDSAEHANGREVYMIYEPMAAALGIGIDVEAPEGSMVVDI
                     GGGTTEIAVIALGGIVCNQSIRIAGDGFTADIQAYMRHQHNIKVGDRTAEEIKIGVGS
                     ALPDLDTPPADFVVRGPNLMTAMPIEIPVSYQEISHCLDKSLSKIEAALVAVLEQTPP
                     ELYADIVTKGIFLTGGGALLRGLDKRLSDRIQIPFHVPEDPLHAVARGTGIALKNIDK
                     FSFLMR"
     gene            35241..36050
                     /gene="mreC"
                     /locus_tag="PK252_00695"
     CDS             35241..36050
                     /gene="mreC"
                     /locus_tag="PK252_00695"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT13253.1"
                     /translation="MNAIIRFIIKHQFVLLFLLLEIIALVLFVQNNGYQKIKYQAFAQ
                     DFDGYLMHKRHRIGQYFGLKEVNNKLSAENLMLRRQIEMLKAEKANKDTAVNFTSYIG
                     ARVIDNSVNKPYNYVLVDKGAKDGVKPEMAVISNQGVVGLVASVSKNYSLVASVLNRK
                     IKVSAKIKKNGYFGSFEWDGLNYRYAYLHDIPYHAEVKAGDTIVTSGYSLIFPENIPV
                     GVIDEVDSTGGNFLNIRTKLSVDYKKLDFVYIIENNSRAEIEGLQKKGSND"
     gene            36043..36549
                     /locus_tag="PK252_00700"
     CDS             36043..36549
                     /locus_tag="PK252_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13254.1"
                     /translation="MIRVLTQNIILFFTVILLQLFVFNHIQLNGLIIPYFYVLLLLLL
                     PFDISKSMLLFVGFVLGIIIDIFSFTPGLHAAACTFAAAVRPTILEWFAPRDGYESGT
                     LPRISYFGLAWFIKYSFVLIMAHHIFLFFLEVSTFHGFFSTLFRAIASSIFTLTLVTV
                     SQFFIFRK"
     gene            36557..38377
                     /gene="mrdA"
                     /locus_tag="PK252_00705"
     CDS             36557..38377
                     /gene="mrdA"
                     /locus_tag="PK252_00705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT13255.1"
                     /translation="MNQYSTRQYIIAGIFLSVFLIFLVRLFYIQIIDSTYKYSAENNS
                     QRYVTIYPARGLIYDRHHKLLVSNKAAYDLMVNPQELREFDTASFCKILNINKEYVDK
                     TLKKIKDYSKYKSSPFLFQVSDTTYAELQEKLYLFPGFYVQARTLRQYQYPIAAHLFG
                     YVGEVDSSIIKKNNYYQMGDYIGMSGLEKSYEEYLRGVKGVNILLVDVHNRVKGSLQN
                     GKLDRPAILGKNLVSSIDADLQIYGEKLIKNFRGSIVAIEPKTGEILAIVSNPTYNPS
                     LLVGRERTKNFIDLRNDKSNPLFYRAIMSKNPPGSTFKLLNAMIALQTGVAVPSTTIG
                     CYHGYTAGPVHVNCHVHPSPLDMPHSIQHSCNAYYSVLFRKIIDEQKLPRNQAYDVWR
                     KLIMSFGLGQKIHTDIANELGGNIPSSNYYDKIYRKSWNSLTVVSLGIGQGEIGLTPL
                     QLANVACAIANRGYYYVPHVVKQIEGIDTIDQRFLKKNKTLINPEYFDTVIEGMYMAV
                     NGPGGGTATGAAVKGLDICGKTGTAQNPHGEDHSIFIAFAPKDNPKIAISVYIENGGF
                     GATIAVPVASLMIEKYLTDSISRPYYEQYLETKRINYPVK"
     gene            38374..39798
                     /gene="rodA"
                     /locus_tag="PK252_00710"
     CDS             38374..39798
                     /gene="rodA"
                     /locus_tag="PK252_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT13256.1"
                     /translation="MKRAFNVWNNLDWFTVMIYLALVIIGWINIYAAVYSDEHQHIFD
                     FQMRYGKQMIWIMAAIFLAIVALVIDIRIYSFFAYPAYLATILLLIAVIFLGKEVNGA
                     RAWFEIGGLRIQPTEFAKTATCLALAKYLSSFEIRIDKVKTMMTILLIIAIPSTFIIL
                     QPDVGSLLVFVSFAFVLYREGMPGGFLFFGFLLAFLFVFALIAQPLTVLIVILFIAVV
                     AFFLLNKGTKETLIFIFTLVGLYFLIFIIAKLFHIKLSYYKTLLIASIITGISGLVYQ
                     FFRKVPYAFWVAVMMFIAIGFTYSIDFVFNNILESHQKQRVNILLGKESDPRGAEYNV
                     NQSKIAIGSGGFFGKGFLQGTQTKYNFVPEQSTDFIFCTVGEEWGFLGSFIVLALFGL
                     LLVRIIYLSERQRSAFSRIYGYGVASILFFHVFINVGMTIGLLPVIGIPLPFFSYGGS
                     SLWAFTLLLFIFLRLDAGRLELFH"
     gene            39931..41799
                     /locus_tag="PK252_00715"
     CDS             39931..41799
                     /locus_tag="PK252_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT13257.1"
                     /translation="MNQLVLVLFILPLLVSPLLLVLPRKARTFATVTTVSILSLISVY
                     LYFRADETISIMLPGIVNTIIAGADVILLLYFGYIAIQRKSLLVGIMSVLQLGGVLFL
                     LTKMPHHEANQILVDKLSLFMFLLINIVSGVIAAFAIEYIEEENCSEYRKRFFLSVIM
                     WFIGVMNLIVSADNLEYFFLFFELTTLASYLLISFRKDEESVHNALTALWMNQIGGIA
                     ILGAIFMAVFTPGYGEITFSSLIAKAPTVGVLLPFALLAVAALIKGAQMPFSKWLLGA
                     MVAPTPVSALLHSSTMVKIAPFVILRISPAIHNTMLAQVIIGLTAFVFLVAAISALAQ
                     DTFKRILAFSTISLLGLMCLMAAIGSEVAVVAALLLIIFHGLSKCMLFLNAGIIERIF
                     HWKKASDMDKLGEVGPFTSLIVAVGFMSLLLPPFGAFVGKWFSLETIGAGLGNSQVAG
                     ALVISAIAIGGAVMSLLYFKVMGLIIARSGEKDKVQFESMPILFSSSMYILLALIFAG
                     IISLPFMMGGFFAPVASDVTGLAVNVQWSKLTMMIGEAQLPIIPLFLAFLFLPLTMII
                     AMFVRFKGADRAKEYMCGEKVEYRLSSFYFNTEKVTPYFIAIGIIFFIGIVTVAII"
     gene            41825..42664
                     /locus_tag="PK252_00720"
     CDS             41825..42664
                     /locus_tag="PK252_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT13258.1"
                     /translation="MNSIIICTIIVILAPFIGGLVYGIERKVKARMQHRVGPPILQPF
                     YDFMKLVDKRAMIVHSAHAFLGIMHFIALWFAVAVLIFGGDFILVIYLHLMATALLII
                     AGYSTRSVFSHLGSNRLAISALAYEPVLILIAISVYLTTGSFEISSIFSMSTPLIAKM
                     PLAFVALLLILPIKLKKSPFDVAEAHQEITGGAEIEYSGIFFEFLYTAKWLDYVFCYV
                     LVYLFAANNVWLGLGLVAATFLYINALDNSTARVDYKQMLKFSMYVALPIAFINLLLT
                     ILK"
     gene            42669..43184
                     /locus_tag="PK252_00725"
     CDS             42669..43184
                     /locus_tag="PK252_00725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOT13259.1"
                     /translation="MKLSGYRKKSPWILHYNAGSCNGCDIEILACLCPKYDVERFGLI
                     NTGNPKQSDILLITGPVTKRSRERLVEIYAQMPEPKVVIACGSCASTGGVFRPMYNIE
                     GGVDQVIPVDVYVPGCACRPEQIIDGVVTALGILEQKTKEIEKSHILHVAPLEEDGQV
                     NRRHMAGTIKK"
     gene            43225..43569
                     /locus_tag="PK252_00730"
     CDS             43225..43569
                     /locus_tag="PK252_00730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT13260.1"
                     /translation="MKKIETTLETLKADIGAFYKVDEHHFIVMNVVDLGGNQLDIQWF
                     FSDYAQPCEVTMFTAKADAGVQIPSINSIVASAWVAEAELVDLMGVNIENAQKGFVLE
                     DDFEGSPLRKVK"
     gene            43573..44661
                     /locus_tag="PK252_00735"
     CDS             43573..44661
                     /locus_tag="PK252_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOT13261.1"
                     /translation="MATQYDIPVGAQHISLLEPLHFKLTTENEKIVDVQANVGYVHRG
                     IELACTSKFKFRQVSFVVGRVCGLCSISHTTAYSQVAEKLLNLELSKRAKYLRILVLE
                     LDRIHSHLLCLSHVAENSGFEALFMKTMKDREISMELLEAVCGNRVQYDYSVVGGVAR
                     DLRPEVAQMVLARLNDFKPKLDDLREIFENNYSLSLKNRGIGVLSSENVKLLNAVGPL
                     ARAAGVPIDVRNETEDLLPWKEVGFNMVLGDAGDVHARNMVRIHELYNSIQMIENVIN
                     GLPEGDIITETKAMPEGEAVVRLEAPRGELFHYAKGNKTQILERLKIKAPTFSNIPAF
                     IETFKGNEYASAPAIIASFDPCLSCTAR"
     gene            44687..45121
                     /locus_tag="PK252_00740"
     CDS             44687..45121
                     /locus_tag="PK252_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT13262.1"
                     /translation="MKSFFEALTNLFRKPATVKYPFEKTYIPEDYRGMIVHNPELCIW
                     CRKCEMVCPPGAIVFSQDMDGKQTYHYNRAVCIYCGECVRQCPKDGALMQSATPGTPA
                     VKNENINNSWKVTYDEALKSRAAYMAAKKAAAASKSAEGAAE"
     gene            complement(45180..45404)
                     /locus_tag="PK252_00745"
     CDS             complement(45180..45404)
                     /locus_tag="PK252_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT13263.1"
                     /translation="MLMDLMLPDTDGIELTKFLINERFKVPIVFISAYTERCEEIYDL
                     GIEYFLNKPVLPELFLSIVSKYIELEEKCK"
     gene            complement(45416..45553)
                     /locus_tag="PK252_00750"
     CDS             complement(45416..45553)
                     /locus_tag="PK252_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13264.1"
                     /translation="MKRVFAIEDNDTNYYLLEEILSEYNVEMVRAADGKEFYNIMQKN
                     R"
     gene            complement(45676..46290)
                     /locus_tag="PK252_00755"
     CDS             complement(45676..46290)
                     /locus_tag="PK252_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT13265.1"
                     /translation="MYKVFFNNSFLVIANKNEADKIKLQAIPFENEKQFIDWLATAQN
                     NNTSETKCFTTEDVEGTFKLFASKFRIIEAAGGLVENADKNFLMIFRRGKWDMAKGKI
                     DKGETPEKAAIREVEEETGAKKLSINRFFQHTYHLYFLKEELVLKKTHWYLMQCKSNK
                     NLQPQTSEDIERALWLPYNEIIPLKPLMHGSIRDIIENYFENKE"
     gene            46330..46962
                     /gene="pyrE"
                     /locus_tag="PK252_00760"
     CDS             46330..46962
                     /gene="pyrE"
                     /locus_tag="PK252_00760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT13266.1"
                     /translation="MEYSKKIAQFLIESQSVKLRPEEPFTWASGWKSPIYCDNRKTLA
                     FPAIREYIKNAFVDMINKKFPEPDVIAGVATGAIAQGALVADLLKKPFCYVRSAPKDH
                     GMANLIEGNLEPGQKVVVIEDLISTGGSSIKAVEALRNAKADVLGMVAIFTYGFESAS
                     QNFKKAGINVYTLSDYDTLLEQAISWGYINPSHLNLLKEWRVAPDKWGNA"
     gene            46964..47380
                     /locus_tag="PK252_00765"
     CDS             46964..47380
                     /locus_tag="PK252_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT13267.1"
                     /translation="MEVKVESRVGKMPVNTEALYRLFSDFRNMTQFIPKDKLRNFQAT
                     EDTCSFEADMVGLVGLKIVERKPNSLVKIISSEISKYSFTFWVQLKEVATMDTRIKLT
                     LAFESNPFMAAMFKGQVEKALNSMIDRIEQQNFSYN"
     gene            47377..48144
                     /gene="rsmA"
                     /locus_tag="PK252_00770"
     CDS             47377..48144
                     /gene="rsmA"
                     /locus_tag="PK252_00770"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT13268.1"
                     /translation="MMFVQAKKKLGQHFLNDQNIALKIVEAIEGQPGLVIEIGPGMGV
                     LTQFLVKKIENFIAIEIDRESVEYLTNNNIISESKLFRQDFLRADLQNLLKNNTCIIG
                     NLPYNISSQIFFKVLENKQQITQVVCMVQKEVAERIAEPPGSKTYGILSVFLQAFYDI
                     EYLFTVHEHVFTPPPKVKSAVIRLKRNWRLELPCDEKMFFTVVKTAFNQRRKTMRNSL
                     RPLYPPEALADPVFDKRPEQLCVEEFIELTKMLEPKD"
     gene            complement(48141..48341)
                     /locus_tag="PK252_00775"
     CDS             complement(48141..48341)
                     /locus_tag="PK252_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13269.1"
                     /translation="MFGSILGGLIIVGFAVGFVYTAFIILHETYKKPIFKYAFMAMIT
                     LLPIIGIIGYWVIYKKKKDWII"
     gene            complement(48347..50110)
                     /gene="sppA"
                     /locus_tag="PK252_00780"
     CDS             complement(48347..50110)
                     /gene="sppA"
                     /locus_tag="PK252_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT13270.1"
                     /translation="MKSFFKTLLASILGVFIAGIIMFFVFIGIMSALVSDMEKATSIE
                     PNSILKLTLENEIVERSSKGILDNIDIGYGQSKQDGLNEILENIEKAKVDPNIRGIYL
                     ELSVIQASIGTISEIRNALLQFKTSGKKIIAYGEYMSQGAYYLASVADEVYLNPEGSI
                     IFKGAQAEVMFFKGALDKLGIEAQIIRHGKFKSAVEPFLNNKMSPENREQLQAMLDAI
                     WNNTVNDIAKSRNISPEKVKKIANTLNTSDADSCVATGIVDSLLYKDQIFAKLLKISG
                     SSNKEPKLVTLAKYKNVPKAVTGEGVAKNKIAVVYAFGDVVMGDAADGYISSEKISAA
                     LRKARKDTSIKAVVFRINSPGGSALASDIIWREVALTVAEKPVVASMGDLAASGGYYI
                     ACPASYIIAQPSTITGSIGVFGVMFNAKKLLNEKLGITTDVVKTEGHSDFPSGTRAMD
                     QYEQKVMLSQIERIYNTFINHVAEGRKMKAEDVDNIGQGRVWAATDALKIGLVDTLGY
                     IDDAIAKAAKMAKVEKYRVVNLPLQQDPWEKFINELSGEVMMKLFTGKYAEYTKYIRI
                     ANKVHSMQGIQARLPFELDAE"
     gene            complement(50227..51390)
                     /gene="nhaA"
                     /locus_tag="PK252_00785"
     CDS             complement(50227..51390)
                     /gene="nhaA"
                     /locus_tag="PK252_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOT13271.1"
                     /translation="MIKKRISETRFSKIFKEFLENEKTGGIILVAVTIISLILANTSW
                     HDAYKSIWGFPVANHNLEHWINDGLMTVFFLLIGLEIKREIYKGELSNIKNASLPVFG
                     ALGGMIVPALLYTAFNIGKITQSGTGIPMATDIAFSIGILSLLGNKVPASLKIFLTAL
                     AIIDDLGAIIVIALFYTSDLSVLNLLIALGIFGILLLFNYFKFKKLFIYLIGGTIMWY
                     FMLHSGVHATITGVLLAFAIPFVDGSKKSPSSILEHALQKPVPFIILPIFAMANTCIV
                     IEPNWFASLTHDNSIGIMTGLIFGKPIGIFLFAFASVALGWSALPKDLKWTNIIGAGI
                     IAGIGFTMSIFITLLAFDDPHLVAGSKIAILLASLTAGIIGYIYLKLNLKNKK"
     gene            complement(51452..52747)
                     /locus_tag="PK252_00790"
     CDS             complement(51452..52747)
                     /locus_tag="PK252_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOT13272.1"
                     /translation="MRTTPINSYFFEKNRQKLLKILPPHSLAIIPAASNIISTADQHY
                     PFRQNSDFYYLTGINHPNAILMLSPDHDKAEFREVLFIPEITEHDKTWNGNILTSEKA
                     TLLSGIKKIMLANEFEAIVSSFAEKIKTFYLPITDIAKTAYDIPVNQRKLAHYIQEVH
                     PLHHFERLSPIIHSLRRIKEEAEIQQIKKAIDITRKGFLSSLKVISPGKREYEIEAEI
                     TAEFIRNGANGHGFLPIIASGANACILHYINNDCICNDGDLILMDIGAEHNYYNADIT
                     RTVPVNGRFTSRQRQVYEAVYRLHNNIISMIKPGISIEALNQQTIPFIEKELVDLKLI
                     TPAELKKQLPEKLLYRKYLMHGVAHSLGIDVHDPNNKHDTLEENMVITCEPGIYIAEE
                     GIGIRLENDILITQDGGINLSIDIPIDADAIESLMHNKY"
     gene            complement(52909..53136)
                     /locus_tag="PK252_00795"
     CDS             complement(52909..53136)
                     /locus_tag="PK252_00795"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT13273.1"
                     /translation="MNSYRDLIVWQKAIELVKQIYALTKQLLKEEIFSLTSQRSAMSI
                     PSGIAERYGYQHTLSGNKVEVLCLHYKPGSK"
     gene            53336..53998
                     /locus_tag="PK252_00800"
     CDS             53336..53998
                     /locus_tag="PK252_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOT13274.1"
                     /translation="MSNLVNSSIGKKLMMGLAGLFLCAFLLVHLGINLLILLNDGGEA
                     YRMGVEFMTTNILIKIMEIFLFGGFLLHIVYAIYVQVQNWFARPIRYAKTNHSQTSFF
                     SKYMIHTGVIIGIFLVLHFINFYFVKLGIVAVPEGAEGKHDFYNMVLNLFKNPVYSWI
                     YVVFMIGLAFHLNHAFQSAFQTLGWNHKKYTPIINFVGTAYSIVVPAGFAIIPIYVLY
                     IM"
     gene            54139..56073
                     /locus_tag="PK252_00805"
     CDS             54139..56073
                     /locus_tag="PK252_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOT13275.1"
                     /translation="MSLNSKIPSGPLEQKWSYYKSHQNLVNPANKRKLDVIVVGTGLA
                     GASAAASLGELGFNVKVFCYQDSPRRAHSIAAQGGINAAKNYPNDGDSVYRLFYDTIK
                     GGDYRAREANVYRLAEVSGNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYSALSRQIEKGTVKMYNRTEMLDVVLVDGKARGIITRNLVTGAIERHF
                     GHAVVLATGGYGNVFFLSTNAMGSNGSAAMQAYRRGAYFANPCFAQIHPTCIPVHGDY
                     QSKLTLMSESLRNDGRIWVPKDKTVSEKLRKGLMKPTDVKEEDRDYYLERRYPAFGNL
                     VPRDVASRAAKERCDAGYGVGATGLAVYLDFSTAIQRLGKHVIEERYGNLFQMYEKIV
                     DENPYETPMMIYPAIHYTMGGIWVDYELMTTIPGLYAIGEANFSDHGANRLGASALMQ
                     GLADGYFILPYTIQNYLAGEIRTPRMSTDAAEFVKTEAEVKERIKKIINIKGKHSVDS
                     FHKRLGHIMWEYVGMGRTRESLTKAIELIVALRAEYWKEVYVPGTNDEFNPELEKALR
                     VADFLELGEMMARDALNREESCGGHFREEYQTEDGEALRNDDKFMYASAWEYKGDAAP
                     EMHKEPLNYEFIEVKQRNYK"
     gene            56211..56966
                     /locus_tag="PK252_00810"
     CDS             56211..56966
                     /locus_tag="PK252_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOT13276.1"
                     /translation="MESKGLNLTLKIWRQENAKAKGKFVTYEVKNISPDCSFLEMLDQ
                     LNETLVEKFEMPVCFDHDCREGICGMCSLYINGRPHGNDSGVTTCQLHMRKFKDGDTI
                     YIEPWRAKAFPVIRDLVVDRSAFDKIIQAGGYVSVNTGGVPDANAIAIRKENADLAMD
                     AAQCIGCGACVAACKNASAMLFVSAKVSHLALLPQGKIEAKERVEAMVSRMDELGFGN
                     CSNTGACEAECPKEIKITNIARLNREYICSKLG"
     gene            57140..58231
                     /locus_tag="PK252_00815"
     CDS             57140..58231
                     /locus_tag="PK252_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT13277.1"
                     /translation="MKEKIEEALRNVNHPAKGTDIVSAQVLQNIEIVGNKIILTMNYG
                     KANDPFANSIKKSVVKILEPVVGSQYIVEFAEVNNRKPLLPEYYTLKEVKNIIAVSSG
                     KGGVGKSTVASNLAIALSHTGAKVALLDADVYGPSAPKMFGEENFRPVMIEKDGKEYI
                     ESLQKYNIGVQSIGFFVSPGEATIWRGPMASNALKQVITQTWWGELDFLVLDMPPGTG
                     DIHLTIVQELPVTGVIVVSTPQNVALADAIKGISMFRSDKINVPVLGLVENMAWFTPA
                     ELPDNKYYIFGKDGCKKLAESLELPLLGQIPIVQSICEDGDNGTPSAMNPFTPQGKAF
                     AELAEAVIAQVEIRNKTLPPTKKVEVTQK"
     gene            58303..58986
                     /locus_tag="PK252_00820"
     CDS             58303..58986
                     /locus_tag="PK252_00820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT13278.1"
                     /translation="MSRRLVIPDIHGCSKTFEALLEKIVFTREDHLFLLGDYIDRGPD
                     SSGVVDFIIRLKTEGYKIVALRGNHEDDLLKASQSFDKDSFVFYVNRFKSGDLLTAEG
                     QIKEEYKTFFNSLPFYFELPDFILVHAGVNFKIQNPFEDKDALIFLRNTDYNAHKAKN
                     RRVIYGHQPTYINDIMAAIDEQRFLIPLDNGCVYNSLHKVYDYRYLGHLLCLDLDSWE
                     LSMERNRDT"
     gene            complement(58983..61034)
                     /locus_tag="PK252_00825"
     CDS             complement(58983..61034)
                     /locus_tag="PK252_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13279.1"
                     /translation="MRVLFTVLSLLFVVQAFSQKKTPPKRDTTYLAEVKLDYHLLADT
                     VAIDTSINHLHNLKNYQKPEKSYSYLTYNGYPSLSNDLSDRLTMNTNFYPSIAMYFRE
                     TPELSFYHLNKTFSSVTYKIAGGLSDKEEYAGVRFAKSLGKNSSIGVDYNFYSYAATN
                     EIPSASNHHFIINYFKKRESIDSYLQLFWNSTTLQESGGVKNDTLINYKNGDFKGVAV
                     NLETAKSRYRGMGFSTFHQLHFLQGINDDSTNFFNKIKSGSITYKLDVNSQKKLYKET
                     EMDTVSFYKHNYYASKISNDSLSLTTIENAVILKSPELWKYLPNFVGSIHVGYYSAFR
                     GCVPDTLIINGTHFNPPFPQKNNIYIPGFDSANAKGAPLYGYRTYTGQLSNDTIVLKG
                     NADKSFSDYYKFWFTAGIEKSIKRMKGKLVWESHLAGYNIGDQRLDARLWMNKDTNSY
                     FSFDAGFVSELHKPSFMYNSLFSNHFMWNKGDNFKREYKQRIYGSISADKLNTTISGN
                     YHIISNMVYFDAEGVTQTSKTLHVASITFSNTLRLWKFETTNAITFQEFDNSYFQVPQ
                     MVYYNSTNIQHTFRFSTGGKLYARLGFDVKYESSYTPETYIPSLGVFALTSANEKVSK
                     TGDYPVVDVHLTFKVKVVSFFFKYSHINAGFYKKNTFVAAHYPLMIPVMQYGLTWNFY
                     N"
     gene            complement(61301..61771)
                     /gene="bcp"
                     /locus_tag="PK252_00830"
     CDS             complement(61301..61771)
                     /gene="bcp"
                     /locus_tag="PK252_00830"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOT13280.1"
                     /translation="MSEIKTGQPAPFFEGVDQDGKKVTLADFKGKKLALYFYPKDDTP
                     GCTAEACSLRDNYDILTKNGIVVIGVSADSQASHQKFKTKHQLPFTLISDSEKKMLKD
                     YNAWGEKSMYGRKYEGILRKTYLIDEKGVIAHIIEKVDTKNHAKQISQLLMTND"
     gene            61910..63565
                     /locus_tag="PK252_00835"
     CDS             61910..63565
                     /locus_tag="PK252_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13281.1"
                     /translation="MLLFIITPSWGQQQRSSQAQTAIQLFDGGQYTEALPLFNNLVNK
                     YPKDNLYKKYLGACIVETNGTLSQAVNYLEMYKVGEKLYWPELYLIKAYLRLFKVEEA
                     RKVLESLKRDMPYFEARKYGLAEMERYLNFLNFNISENEYGQVAEKVLLNIDILKNNA
                     EAWFDLKYLKIGADSFCLVLVKNEELQPNTTYLLRKMPSKDNALYMSRFSAFGQWQQP
                     EKFDAAFEGFTISNVFPPYIDQQKHVAYFSLEKKSQPTGFDIYMAEFTPKNLKQVKLK
                     ILPLPVNSPWNEYLYISFDTLSLLVSDRETSPQKAVQYLLQKQTKIDLANFKSYELYN
                     FCFFKKSITKISLPQQKIETSKVLPSSYQDEEMVTQALEKQRIADSAMFVAYQIKIKI
                     ANTEDKKMRTTLFGEMKKAEKRAKTLQDEANGMYQQFIPALSAKSQEINKFSEVMSKS
                     KNSEGDVVTKSDGSLVSYRIQLGLFSNPKPAVFFAGIDSVTSENVDNGKALRYFTPYY
                     GTYPDADSMLTIVKSKGFKEAFIVAYYNKRKIPLERARQIEKE"
     gene            63702..63989
                     /locus_tag="PK252_00840"
     CDS             63702..63989
                     /locus_tag="PK252_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOT13282.1"
                     /translation="MKKFDEFSHSPQFDEDIEKTNGLANFLTLHNDDLHTFEYVIESL
                     VEVCKHDFEQAEQCTYLVHYKGMCDVKKGSYHSLIPYKKELSRRGLKVTIN"
     gene            63999..64469
                     /locus_tag="PK252_00845"
     CDS             63999..64469
                     /locus_tag="PK252_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT13283.1"
                     /translation="MTILTIVLLVALAIFLLVLELFIIPGISIAGIGAILVYAGAIAI
                     AYIKLGVVGGNITLAASIISSAIAVYIFFKPSTWKRVTLSTEITAKVPEYEVCSLLKP
                     GMQGVAKTRLGPMGEIEINDIITEAQSMGAIINANTPIEIVYIEDHKIFVKPLI"
     gene            64476..65465
                     /gene="floA"
                     /locus_tag="PK252_00850"
     CDS             64476..65465
                     /gene="floA"
                     /locus_tag="PK252_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134940.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOT13284.1"
                     /translation="MEIGIYLFVGAIGIVLLWIFFYLVPIGLWFQGILTGVKVSLLQL
                     IFMRWRRVPPSVIVNAMINSKKAGLDLSPDQLEAHFLAGGHVQKVVNALISADKANIP
                     LDFRAATAIDLAGRDVFEAVQMSVNPKVIDTPPVTSVAKDGIQLITKARVTVRANIKR
                     LVGGAGEETVLARVGEGIVSSIGSSASHKEVLENPDKISKTVLAKGLDAGTAFEILSI
                     DIADIDIGKNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKSVEARANVIQ
                     AEAEIPKAFADALRSGNIGVMDYYKIKNIEADTNMRDSIGKSGIQEDPSKKKQ"
     gene            65538..65624
                     /locus_tag="PK252_00855"
     tRNA            65538..65624
                     /locus_tag="PK252_00855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65572..65574,aa:Ser,seq:gct)
     gene            65797..66489
                     /locus_tag="PK252_00860"
     CDS             65797..66489
                     /locus_tag="PK252_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT13285.1"
                     /translation="MGALYDELAKDIHLQEGLNACINCGTCTAICPAAGFYNYDPRQI
                     ADIVQQQDDKQIEELLKSETIWYCGECMSCKTRCPRNNTPGQIIIALRSYAQEHGYFT
                     QSEKGRQQIYLKRTVGQWILDHGYCLYLEGVGTDNHPEQGTIWDWRQDNWTETMDRLG
                     ANYQKAGAGAMRKIPEEALEEVRKIFEVTGGMERFRKIEEYSAQKASEMGLEDEEYVK
                     EVYTTNSSQDNK"
     gene            66499..67575
                     /locus_tag="PK252_00865"
     CDS             66499..67575
                     /locus_tag="PK252_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOT13286.1"
                     /translation="MKVEGKARIWKNYQKEIPDNDFFYVRSCVRQNFFPGAETTFLRI
                     MKDELGINVYDDPAHTSCTGIGYHADIVPFDTTMTVVARQFALMTEKGFKNMIPSCIT
                     SFGLYTEILETWEHHPEVLEKARKYLKEATGRTFEIPENLSHASDVIFKFRHKIAERA
                     KYKLINKYTGKPLRVVEHIGCHYAKMFPSKGVGGAEYPYVLTGMVESFGGEIVDYPER
                     RSCCGFGFRQYLVMANRGYTIANTKKKFDSMDPYKPDCIVTNCPGCPMFLDKGQYAIA
                     ELEGRVYGDGKYGIPVLTFEELAGIVLGYDPWDIGLQVHQIPVEPLLDKIGVEYDKSA
                     KYKGIGGRDLGKPQHSKLIKAYIE"
     gene            67578..68606
                     /locus_tag="PK252_00870"
     CDS             67578..68606
                     /locus_tag="PK252_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT13287.1"
                     /translation="MAESKKIAVIGGGIAGMEAAGNLVNLGFEVYLIERTNRLGGNVA
                     NWYCLFPDFKPSSKVVEQLKPAIQNKVQFRFGAEVCKTEKKGNKHLLHLNDGEVLECD
                     AVVVASGFSLFDAHRKEEYGYGIYDNVITSADLEKQLAENHTVMTTQHKAPRKIAFIH
                     CVGSRDHKVGNAYCSKVCCVTGVKQAIEVAKMHPEAEIYSFYMDLRMYDRQFEDLYME
                     AQTQHNVRFIRGRLSECFENADKTILLKAEDTLSGKPFRLSVDMVVLLVGMQADQSLK
                     HISDMFALKTSDDRFLSVADVHTSSIYSGVEGVYLAGACTGPKSIHETLNSARAAALS
                     TAIYLNQN"
     gene            68612..68944
                     /locus_tag="PK252_00875"
     CDS             68612..68944
                     /locus_tag="PK252_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT13288.1"
                     /translation="MKNWGYSIHKDNQIDLDRNDTSVYKALILVEPSIARCISCGSCC
                     ATCSSGEYTSLSFRKIMIQISRGETKDLYSNLSQCMLCGKCILVCPRDVNTRNAITKL
                     LQILTTKN"
     gene            68949..>70456
                     /locus_tag="PK252_00880"
     CDS             68949..>70456
                     /locus_tag="PK252_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT13289.1"
                     /translation="MLFDWFVIPFSIGLVFVLGYTIAKYISWVHQLDPKDKKAFFAHL
                     FSKGIFFSIKEIFSESLLHRKIFKVNKKLGFMHMSLAFGWFLLIALGNIEIKFYAEYA
                     INPPYVPIFLRFFQPDPAPHFFGRGANFLMDLLLLMILVAVGLAIYKRFRPGFFGMKK
                     TTVLHVPDKIALYALWCIFPLRFFAESFTSGVYNNGSFLTGSAGSFFAAFLPVENLYY
                     PAWWAYSFALGTFFLFLPFSRYMHILAEVMLIVMRNAGVKACIKNDVYHQVQIISCSR
                     CGVCIDKCQLSKVNDYAIQPSYFIREVRYNKLQQTTTENCLMCSRCDSICPVGVESVD
                     IRLRKRAEGNNITSMGYEKIKPVDLPHKSIDILYFAGCMSHLTPAVKASMKKILKASG
                     QTWQMMDENATICCGRPLQLNGQFEAAQNLRDKNAKLIKQSGAKLLVTSCPICFKTFK
                     DDYNLSIQVVHHTQYIASLIDKNVLPIEQTDGRFSYYHHPCELGRNSGVYEEP"
     assembly_gap    70457..70556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            70751..72481
                     /locus_tag="PK252_00885"
     CDS             70751..72481
                     /locus_tag="PK252_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT13290.1"
                     /translation="MNWTSHEFIWLDWLIIAVGILAIAWAVWRSIQKHKRTLAGASSE
                     DYLFGKGEPWYIIGAAIFAANIGSEHLVGLAGTGAKSGVGMAHWEMQGWMILILGWLF
                     VPFYQLMNNKLGKIITMPDFLKTRYTHRTGSWLSIITLIAYVLTKVSVTAFTGGIFME
                     SLLGLPFWYGAIGLIVLTGIFTVVGGMKGVMTLSAIQTPILILGSFLVLFLGLAALGD
                     GSVVHGWTAMIEHAKTLNNGYGTNHMFHFETGDPMYDDYPGFWVFIGASIIGLWYWAT
                     DQHIVQRVLGQSKGEPNDIVMKRARRGTIAAGYFKLLPVFMFLIPGMVAAALAARPGS
                     GFTLENPDTAFGSMVKFVLPAGVKGIVTIGFISALVASLAAFFNSCATLYTEDFYKPM
                     FKGKTEERYVLVGRIATVVVVILGIVWIPVMMSLGSLYFYLQRIQSLLAPAMVAVFLL
                     GIFSKRITPKAGELGLIVGFFIGMIRLLTNILTNTGSNVMTGWYWEATAWFWQTNWLI
                     FEIWLLVFIMLFMVAVSFFTPKPTPQQIDAITFTSDYKEQIRKSWNKWDVIASLGVIL
                     FCALFYYYFW"
     gene            72612..74117
                     /gene="araA"
                     /locus_tag="PK252_00890"
     CDS             72612..74117
                     /gene="araA"
                     /locus_tag="PK252_00890"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOT13291.1"
                     /translation="MKAFENFEVWFVTGAQLLYGGDAVVAVDAHSNEMVKGLNDSGKL
                     PVKVVYKGTVNSSAEVSATFKAANNDKKCIGVITWMHTFSPAKMWIHGLQELKKPMLH
                     LHTQFNKEIPWDTMDMDFMNLNQSAHGDREFGFINARMRRPRKVVVGYWQDANTLARI
                     AVWMRVAAAWADAQDMVVVRFGDNMNNVAVTDGDKVEAEMRLGYHVDEYPIGKLVAVM
                     NKVTEAEIAAMLKTYEAEYILADNVKEGGKDRQRLVEAAREEIAIRKFLESKGAKAFT
                     TSFDELEGLDQLPGLASQRLMAEGYGFGAEGDWKTAALCRTMWFMGQGLEGGCSFLED
                     YTLNFDGEKSSILQAHMLEVCPLIAENKPRLEVHPLGIGGKNDPARLVFTTKPGTGVA
                     ATIVDMGNRFRMIVNQVDCIKSKALPKLPVASALWIPQPNFEIGAGAWILAGGTHHSS
                     FSFALTQEYLEDYAEIAGIELVVIDKDTTISNFKKELRMNDVYYMLNKALS"
     gene            74212..74901
                     /locus_tag="PK252_00895"
     CDS             74212..74901
                     /locus_tag="PK252_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOT13292.1"
                     /translation="MLEELKKKVFEANLELVRQKLVIYTWGNVSGIDRSQNLVVIKPS
                     GVSYDDMKAEDMVIVDFDGNVVEGKLRPSSDTATHIELYKAFSGLGGVVHTHSTYAVA
                     WAQAQKAIPAFGTTHADHFYGAVPCTRPLTKAEVENNYELNTGKVIVETFAKIEPLSV
                     PSVVVAGHGPFSWGETPEKAVYNAVVLEEVAKMAMITTRLGGEKPIDQYLLDKHYNRK
                     HGKNAYYGQKK"
     gene            74988..76670
                     /locus_tag="PK252_00900"
     CDS             74988..76670
                     /locus_tag="PK252_00900"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOT13293.1"
                     /translation="MSKYTLGLDYGSDSMRVVIANVENGQVVGTAVSEYARWKAGKYC
                     VPAKNQFRQHPQDYIDSMEQAVKEALSKAPAGTAKDIIAISIDTTGSTPVAVDKNGTP
                     LSLLPEFAENPNAMFVLWKDHTAVKEAEEINNLARKWKIDFTKYEGGIYSSEWFWAKL
                     LHIMREDEKVRAAAYSWVEHCDWLPALLTGNTKPENLKRSRCASGHKAMWAEEFGGLP
                     HEDFLVALDPVLKGMRERLFSENTYTCDVKVGTIAPEWAKRLGLSENVAVGVGAFDAH
                     LGAVGAQIKPYQLIKVMGTSTCDMLIAPEKDVHGKLVKGICGQVDGSIIPGMIGLEAG
                     QSAFGDVYAWFKRVVVEPAAKLIEQSSAISVDQKAKLIQELENKLIPLLSDEAAKIDP
                     ADTTVIALDWMNGRRTPDANQMLKGAITGISLGSDAARIFRALVESTAFGAKKIVDRF
                     ISEGVKIDGVIAIGGVAKKSPFVMQTMADVLNMPIQVVVSDQACALGSAMAAAVVGGA
                     YKTVEEAQEAMGSGFETTYHPNAKMVEVYKDIYARYSLLAETLEPLYNAQKI"
     gene            77083..77155
                     /locus_tag="PK252_00905"
     tRNA            77083..77155
                     /locus_tag="PK252_00905"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77116..77118,aa:Met,seq:cat)
     gene            77551..78942
                     /locus_tag="PK252_00910"
     CDS             77551..78942
                     /locus_tag="PK252_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT13294.1"
                     /translation="MTNKIKTISCCLVILAGVNFLAKAIDKASASYHDMATEHLFQNL
                     KALGDAGLHLFGVANAFDISYKKLIHEYDGSTDCKDITGSHPAFIESDFEWYIHKEHK
                     AKWATEAIREAYKQGIVVGYCYHLRGLKSNDFYAFKDNKKTEDSTLLQDIVANPDRKA
                     NKALDWYLHRLDSVVIPTFKELGFPLIYRPFHEMTGGWFWWGTSCCTKDEFIKIYRLT
                     VDYLRSNGVRNVLFAWSPDKSSDSTFYPGDEYVDIIGYDGYDVGLVDYNQIPAFVSNV
                     AALADFAQRHNKIVAITETGTGKLLEAPEYWTKNILESLNADPKASKVSWIMTWYSAD
                     WNKDNKGVNYIPYKGIEKKTNGDKAIDDFKKFYNDPATVFQVDSLPLFATDNAELFIY
                     PKNLTLTVGKKFTLIGGRKKDWSKNQVSWLSSDPSVVKVDAKGNIEALKTGNTTITIK
                     MGNKQASCIVVVQ"
     gene            complement(79003..79950)
                     /locus_tag="PK252_00915"
     CDS             complement(79003..79950)
                     /locus_tag="PK252_00915"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT13295.1"
                     /translation="MQAITKTDFHFAGQTGKYTGKVRDVYNIGGKYLVMVVSDRISAF
                     DVVLPKGIPYKGQVLNQIAVKFLDATTDIVPNWKIASPDPMVTIGHFAEPFKVEMVIR
                     GYLTGHAWREYSAGKRTLCGVTLPDGMKENQKFPKPIITPTTKASEGHDLDISREEII
                     AQGIVSEADYSKLEEYTYKLFERGTEMAAKMGLILVDTKYEFGKKDGQIMLIDEIHTP
                     DSSRYFYARGYEDRLAKGEAQKQLSKEFVRQWLIENNFQGKEGQTVPNMSEEFVAQVS
                     DRYIELFEKITGDKFAKADNTDIIARIEKNVSTALVNLK"
     gene            complement(80099..81469)
                     /locus_tag="PK252_00920"
     CDS             complement(80099..81469)
                     /locus_tag="PK252_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT13296.1"
                     /translation="MKHPSILILGMIVMLVLVAFACTRTTPEKVKPAPTTVKNLEWVK
                     NAVIYEVNVRQYSKEGTFNAFAKDLPRLKSLGIDILWLMPINPIGKLNRKGPLGSYYS
                     VQDYKAVNPEFGNMDDFKNLVKKVHEQGMKIIIDWVPNHSSWDNPMIKEHPEYYLKDS
                     TGKMVSPFDWTDVVRFDYNNADMRKWMIETMKFWLTETDIDGFRCDVAHMVPVSFWDS
                     CRIELDKTKLIFFLAEADQPFLHANAFDASYDWKFHHIMNQTAKGKKNANDITKHFNW
                     VDTAYPQGSILMQFTSNHDENSWNGTEYERMGDGAKTFAALTFIVPGMPLIYTGQESG
                     FNRRLKFFEKDSIDWKDYPLTSFYQKLISIKKDNKALWNGFAGGSLQRINLTDNKHVM
                     AIQRTKETNTVIGIFNFTSKTKNFAIESYISGNYTEAFSGKNIELHQYKKMQLAPWEF
                     LILVKK"
     gene            complement(81481..83340)
                     /locus_tag="PK252_00925"
     CDS             complement(81481..83340)
                     /locus_tag="PK252_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOT13297.1"
                     /translation="MSKKILTFILLMSLSIVGMYAQPSIERIEPANWWVGMKNPHLIL
                     LVYGKNVAKSNVKLADNSIALLEKIEKTDNANYLFVYLSVKSKAKAGDIAISFSGREK
                     DTVVKYSFLERKSKADGKAGFNPTDAMYLITPDRFANGNPKNDSIAGYTDTTDRRKPD
                     SRHGGDIAGISQHLGYISKLGFTTVWINPLLENNMPQTSYHGYAITDFYKIDPRFGSN
                     KEYCDFVKKAHDMKLKVVMDFVLNHCGSSHWWHNDLPSKDWYHQFDSFTRTSYRASTL
                     IDPYASQYDKAVFEKGWFDNTMPDLNQKNPLVATYLIQNTLWWIETSSIDGIRLDTQP
                     YSDMEFLNDWAGAVNAEYPDFSIVGESWFSYPAFTACFQRNQKPISSYSSQIGHVTDF
                     PLCFAMLASVNDSDDWDNGMAKVYNTLCMDFLYADAMQNLIFLSNHDLSRLEETVKGD
                     IHKYKMLVTMLATLRGIPQFYYGDEILLPGDKAKGDGRLRKDMPGGWPSDKVNVFTND
                     GLSAIQKEALDFTRQIFTWRKNNPEIAKGRLVHFVPNNGLYVYSRIAKNDDAIVVFVN
                     NNKKPVKVDSKVYAELLKGAASAKNVITGEEYNLSDFSVAPKTAIIIELKDKN"
     gene            complement(83529..85856)
                     /locus_tag="PK252_00930"
     CDS             complement(83529..85856)
                     /locus_tag="PK252_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOT13298.1"
                     /translation="MKNIPMNKYLKTDPWCIIEEEFNAERQEASESIYSIGNGKMGHR
                     ANFEEHYSGGSLQGNYFAGVFYPDKTRVGWWKNGYPEYFGKMINGTNWIGIDIWFENE
                     QLDLAKCKVSQFKRVLNMKEGLLSRSFYAEMPSGKMVQVSAKRFISIANTRMAAISYS
                     ITPINFTGKAVIKPFLDADVQNKDSNYNEKFWDEVSKGETEGVATLQVKTKKLDFHVC
                     SAMKTNFFVSGKKLPVSATVEQKEKFIAQSFDIPVKQNETLTIVKYVANTTSLDVDIA
                     SMAAELKKDVVEMEQTGFDKLFEAHRLAWEKKWEKSDIVIEGDDEAQQGIRFNIFQLN
                     QTYTGEDERLNIGPKGFTGEKYGGGTYWDTEAFCFPFYLNTSNSKVARSLLIYRYKQL
                     QKAIENAQKLGFTNGAALYPMVTINGEECHNEWEITFEEIHRNGAIAYAIYNYILHTN
                     DYAYIAEYGLEVLIALSRFWAQRVNFSNDKKQYVILGVTGPNEYENNVNNNWYTNTLA
                     KWTLKYTMENIKMVAKDFPQQYEALVKSINFNEANETKLWNDIAENMYLPSIPGTNIR
                     LQQDGFLDKELKSADSIPKEQRPINQHWSWDRILRSCFIKQGDVIQGLYFFINDYTVE
                     TIRDNFNYYEPMTVHESSLSASIYSVIASHIGNIDKAYELYLRTSRLDLDDYNHEVDE
                     GLHITSMAGTWLAIVEGFARKRIINNTLSFEPLIPEKWNKYSFKIQFRNHLLEIIVSQ
                     HDVLIHNHYPEPISIMLYGSPFTISANSKLHYQKK"
     gene            complement(85853..86518)
                     /gene="pgmB"
                     /locus_tag="PK252_00935"
     CDS             complement(85853..86518)
                     /gene="pgmB"
                     /locus_tag="PK252_00935"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOT13299.1"
                     /translation="MIKACIFDLDGVIVDTARFHYIAWKRLANMLGFEFTEHDNERLK
                     GVSRMDSLEILLSVGNITNLTHEKKLEYADMKNKWYVEYISTLTPADILPGAIEILEF
                     LKSHNIKTAIGSASKNAMSILQGLKLEHAFDTIVDGTRIAKAKPDPQVFLTGAYDLKV
                     SPGQCVVFEDAEAGIEAAIRGGMKCIGIGSPVQLGKADIVVPSLDKLNFDIVLSLDSI
                     TKQ"
     gene            complement(86546..87901)
                     /locus_tag="PK252_00940"
     CDS             complement(86546..87901)
                     /locus_tag="PK252_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT13300.1"
                     /translation="MKQKPHLSFWQIWNMSFGFLGIQIGFALQSANVSRILSNFGADL
                     HSLSFFWLAAPVMGLIVQPIVGAASDRTWNRLGRRSPYILAGAIIACMAMWIMPNSSA
                     LVSIMPVLAVGAVMFALMDGSFNITMQPFRALVADMVSDEQRTLGYSIQSFLINTGAV
                     IGSVLPYVLTNIIGVQNTAAEGHVPPSVIWSFYIGGAILLLSVVWTVFRTKEYPPKEY
                     CEQNGIDYDAWSSKKKEKKSVAERLKLFFKLFAEMPPVMIKLAVVQFFSWFALYLMWV
                     YTTPAVAQHIFGTEIGDSSSKLYQEAGNWVGVLFGAYSLFAAIFSLFMTRIAKKIGRK
                     ATYSSALLFGGLGYLSIYFFNTPNALFVSMAGIGIAWAAILAMPYSLLSGALPAEKMG
                     VYMGIFNFTIAGPQIISGLVGGPILRSIFNNQAIYMIIICGVSMLLGSIFVSFIKEKA
                     H"
     gene            88084..88920
                     /locus_tag="PK252_00945"
     CDS             88084..88920
                     /locus_tag="PK252_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13301.1"
                     /translation="MIEICLFDQSKFSAPGNCLFQSQNDDDWKKLYPADAEGNCELAN
                     KVLIIKKENIVVLVDSGFSRYDTASLNEYRVKNFQYIGDILKQASLTTQHITHVVHTH
                     LHLDHCGESIDFSESGEGIKRFSGALYVATGNQLKVATDIDGAGFDSFYTPVVRAFNN
                     NCCDIPLSNDTFLFPWLELRIFNGHTRGLIVPVLHGKEKTLVFAGDLIPTMAHLTLQL
                     ASRYDVNPLLTLAERDDFLDEIIQNEYIIAFQHDPFHEACKVKRSKEEIIAIECKCPF
                     YL"
     gene            89091..90323
                     /locus_tag="PK252_00950"
     CDS             89091..90323
                     /locus_tag="PK252_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13302.1"
                     /translation="MKNFFSILIAMFIVSNTIQAAQKIDTVFTASGKKHKLYYSIPDN
                     YDASKQYPMVVGLHYCGGSGIQYRNALTLLSDSLNYIVVCPDNSSNQIPDANIDIITA
                     AADTARSAFNIDAGSMYLTGMSCNGEVTFRQGMNKFYPFRGIFPWVPWISDNNFSAYK
                     LDSDMPIVLAVGTYDSNFKTLMSLYDSLKANAANVNLVLVPGVGHTLSFNEFGATMIH
                     CIKYLNSAKNIEIAAAKPISMINTDEEKQVEIPITASNSKNIKVKQVLSVNGVFESTT
                     TYDKTSNKAIVKIKPVAGKSGVGKIVIEASEAGDSAIAQTVINVRVDKNVSVKNEEMK
                     ALKTTLAPNPAKGQVVLSTPSTANSFELLDISGKILIQVKASGSQTMLDISKYQAGVY
                     FVKTNYSNTSTLEKLCIQ"
     gene            complement(90438..90511)
                     /locus_tag="PK252_00955"
     tRNA            complement(90438..90511)
                     /locus_tag="PK252_00955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90475..90477),aa:Ala,seq:ggc)
     gene            complement(90848..91348)
                     /locus_tag="PK252_00960"
     CDS             complement(90848..91348)
                     /locus_tag="PK252_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT13303.1"
                     /translation="MSNVQNPLKKVLIICAKGNLEDVYAALVLANGAVMEGIEAKMFF
                     TFFGLDAITKKTMNSLHTATVGNPAMRMPGGLPFPTLLGALPGVEAGVSAMMRSQMDK
                     LDIPPVKEFLEMITAGGGEVFACKLAADMFKLKKEDFTEEVSDIITVGQFYEKCAGEG
                     VQVIFV"
     gene            complement(91366..91677)
                     /locus_tag="PK252_00965"
     CDS             complement(91366..91677)
                     /locus_tag="PK252_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOT13304.1"
                     /translation="MAEKVIAGVTVKVNDEGYMTDPSQWNRDIAVEIAKEYNITLTDK
                     HFAVLEFLRQRVMAGEALTIRRVGSSGIVDIKEFYQLFPGGPLKVSSKIAGIMKPLSC
                     V"
     gene            complement(91760..92995)
                     /locus_tag="PK252_00970"
     CDS             complement(91760..92995)
                     /locus_tag="PK252_00970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOT13305.1"
                     /translation="MGKIVIIGGGTAGTMIANKLYKTLPVHEWEITIVDPDEKHYYQP
                     GFLFIPFNKKLKDKFVKPKKDYIPKGVNFIVDKVSKLEPARKAVLLKSGQELTYDYLI
                     VATGTKPSVDDTPGLNGPLWQKEAFEFYTYEGAVALAEKLETWKGGRMVMAIVDMPIK
                     CPVAPLEFVFLADAFFTQKGIRDKVEISYVTPLPGAFTKPVATAKLSELLSSKNINII
                     QDFYIEHVDNEGKKLVSYDEKEVPFDLLVTVPLNKGAEFIQNSGIGDDLNYIPVNKET
                     LQMQQYPEIFVLGDAAAIPTSKAGAVAHFAADVLLENLLDMIEGKEPTAKFDGHANCF
                     IETGFGKAALIDFNYDQEPLAGTFPLPVIGPMSLLKETRLNHWGKLAFEWIYWNILIK
                     GRYLPISNKMSMVGKKISK"
     gene            93178..>93635
                     /locus_tag="PK252_00975"
     CDS             93178..>93635
                     /locus_tag="PK252_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT13306.1"
                     /translation="MGEYQCSCNGCELRSLFFENLEKGAYESMCAKKQQQLYPKGSLI
                     YRQGEKISHFSYLKSGLVKLVRNIDDKNEQIICFARPLDFVSLLSIFSETCHQYSVIA
                     LEDSEICSLYLDEVKEMAMQNGRFALSLAEKINRASDNIILTMLNIRQRRL"
BASE COUNT        28305 a        16527 c        18958 g        29745 t
ORIGIN      
        1 tgcaggtaca aatggcacaa atttattttc tgacataatc taaaaatttt ctaagaatta
       61 gttgcctgta gcactaaagc ctcctgaaag gcttttgggt tttgggttaa ctaccattac
      121 cggaatttta gccgagttgg caatgatgag ttgttctgac ggccccagca tgtaatccat
      181 aaaagtcagg gtacgtgatg tcatgatcag aataaggttt ccttgggctt gttgtgcata
      241 ttctatggta tcctttgcaa aattaacttt attgccaaca gtttttattt caaaccggat
      301 atcggtattg tcaaaatatt ttttggcaaa aacaaggttt gaataaatac gtcccctgaa
      361 aatacggtca gtaaccaacg ggtgtacaat atggatacga gctttgtaca aattacataa
      421 gtaatgagcc cattttattt tctctttgtt ttcttttttg aaatctatag ggaaaattat
      481 attgtcaagc ttttgtgaag caggcatatc ttgtacaaca agaaacggga cctttgatcc
      541 tctaataact ttcaaagccc aactgccagt aaatttttga atgcctttaa ttccatgagt
      601 agccataatt accatttcgg tattaccttc agaagcataa ctactgatag ttttaaaaat
      661 gctgccaacc aaaattttaa tcaaaggttt tatgaaatac gttttttcaa gtttagaagc
      721 aacagcttct aattgttctt gaacagaact agcttctttt tcatttttta taatatgaag
      781 aagtgtaata ctgtttcctg taatttttga cacattaaca gcatgtgcgt atgcaaactc
      841 acattttggg gaaaaatccc aaagtaccag caaagtttta tctttcgctt ccatttcccg
      901 ttttttcaaa taatcacaaa agtatttgat aaagatagta tttttgatta acattacaac
      961 aaaaatgtaa ttagcctcta atttatgata tttattagca aaaagaattt tttttcttgc
     1021 ttttttttag gttgttttat gttgttttgt gcagttacat ctgcacaaca acctgaaaat
     1081 aatatcattc agggctctcc ttttattcaa aactttactt tccctgaaag gtctttttct
     1141 tctggcaaaa tttctgtttt tgaaagtcct gatggcgggt ttgttttggg aggaagaaat
     1201 aaaataatac ttttttatgg taacgaattt atttctatgc cgctaaatgg acaaattaat
     1261 gtcattggcg atgataaaac ctccttttat acagggttta acactgtggg ggttataagg
     1321 attttcaaaa actcattacc taaactaata ccgttagtgg acgataaaaa aaatcataaa
     1381 aatcgttttg ggcaaataaa caaagttttt attaacgata acgttttggt atttaataac
     1441 aggaagtatt tatatcagtc tgtagatggg tcaaatattc aaatcattga cagttcaaaa
     1501 gtagagttac gcctttttaa agtaaataat gcattactgg ttactaaata cgaaaaaggt
     1561 atttgtcaat ttacacaggg catcattgat aatatccctg gtggagaact ttttaaaaat
     1621 aaagatatag aatgtattct gtcattttgt ggggcattac tggtgaagat aaaaggcgaa
     1681 aaagattttt gtgtattgaa aaacgggcaa gttgcccctg ttcaatttgg ttttgaggat
     1741 tttgtccaaa aaaatggatt ttcagatgcg gttattcttc cttcgggtga tattgctatt
     1801 gctacaaaga gttctgggct tttaattttt aataaaaaaa ctaaaagcat tgtaaaaata
     1861 tctgtcgaag acggtttgcc agagaattca ctcattaatt tgtatgttga caaaacaggg
     1921 aatttgtggc tgatgcacaa taaaggaatt agccggatag aattaaatat ccctgttagt
     1981 gcatatggat ttaatgctgg tctttctggc aatgttaatg atatcctgaa gtttaaaaac
     2041 agattgtatg tggcaactca aacagggttg tattatgcag gtttttcagg aaacaataac
     2101 aaaaaacatt ttgataatat taggtttaag catattgaag atatttcggg tgaggtaaat
     2161 tgtctttttg ttgaaaaagg gaatttgcta tttaccacac ctaacggaat ttttaaatta
     2221 aatgatgata aagtcaaaac aatttttttc gatgagataa aatcttttga aagaattcaa
     2281 tttcaaaatg aaagtttctt ttgtattgca aaagaaagta gtttggatgt ttttaaatat
     2341 caaaatgagc aaatagatac tgttgttatt attaacatgg ctgacaaccc tccatcattg
     2401 gtcaatgaag acaatggtat tgtgtgggtg aagacagaaa acaacgactt attgtacctt
     2461 gagaaggata agctgggaag cattgtttcg gcaagtgatt ttgttcagat aaaaaccttt
     2521 tcggggaata attttggagt catcaagact gaaagcggat tacgttttac attagctgaa
     2581 aaggttttta ggtatgataa gttccgaaaa atatttgttg aagaaaaaat aactcttttc
     2641 ggagtagaag aaataccgag gcctgtaaag atgattaccg atgtacataa caaccagtgg
     2701 tataatcttt caggttcatt acaagggttg aaaggtattt tattatatca gaagattggc
     2761 agggatagtg taaatactgt atttttcaat acaggtttag atgtgaataa aatctttgta
     2821 gatacaacaa gtgtatggat tgctgcttct gacaaattac tcagatatga ccttcaaaga
     2881 aattacaagc cccaacggca gttttcggct attattaaaa gggtcatcat tgggcaggat
     2941 tcaatattgc ctgttgaact tgaagaccct gaaatcaaat atagcttgtc aaatgttagg
     3001 tttatagtgt cttctacatg tttcgaaggc gagccttata ttaggtatca acacagaata
     3061 ttaggaaaga gcaaagaatg gtcagcctgg gatagcaaaa gtattattac atcagggaaa
     3121 ctatctcctg gagactatac ttttcaggta agggccatta gtattgaggg gcgtgtatct
     3181 gaaataactg aactccactt tacggtgctt tcgccttttt ataaaaccat accagcctat
     3241 gtggtttatg tagtattgtt aatgtttttg atttttgttg ctttaaggta caaaacatgg
     3301 aggtttataa agtataaaga agggattgaa aggattgtcc aggaaaggac caaagacatt
     3361 ttaaaagaaa aagaaaagag cgagatttta atagctaatc ttttgcctaa aggtacggca
     3421 gatgagctaa aacaaacagg aaaagctact tcgcagaaat ttaacatggt aacagtgtta
     3481 ttttctgata ttcagggttt tactaaaatt gcagagcaaa tgaaccctga tgtgcttatt
     3541 gaccagttag atgctttttt ctttcatttt gacagtgttg tcgaaaagta taacattgaa
     3601 aagattaaga ctataggaga tgcatacatg tgtgccggcg gtattcctga taaaaacatt
     3661 accaatccgg tagaagttgt tttggcagcg ttggaaatgc aggaatacat ggcaggcttg
     3721 aaaaaaaata attcggacat ttgggattta cgaattggga tacatacagg atctgttata
     3781 gccggggtag ttggccataa aaaactttcg tatgatattt ggggtgatac ggtaaatact
     3841 gcaagccgta tggaatcgtc tggagaagct ggcaaagtaa acatttcggg ccaaacatac
     3901 gagttggtta aagatttttt tatttgcgaa tatcgaggca agatgcctgt caaatataaa
     3961 ggcgagattg atatgtactt tgtaaaaagt ataaggcccg aattgtcaat agatatgcac
     4021 atacagccca ataaaaagtt ttttattatg ttacagttgt tgaggcttca ggatgttcaa
     4081 gacgaggtga tagaaaaatt aataaatggg cttcctaaaa acctttactt ccataatgta
     4141 gaaagggtca aagaattgtt taaccttgta gatttatata gtagggcaga ggaaatgagc
     4201 gatgaagata agcttgttgt aaaaacggct gcattgcttg cagatgttgg ttacatccag
     4261 tcatatgatg aacatgaaga gtacagtatt gagtttgcca aagatttttt gccacgtttc
     4321 aaatacaacc aggaacaaat agaaaaaata tgtgccctta ttgatataac caagcagaat
     4381 cggaagcctc aaaataagct tgaagaaata ttgcttgatg cagagatgaa ctatttgtcg
     4441 cgggctgatt ttgtcagtct taatgaacaa ttgtttaaag agcagctcga acatgggaaa
     4501 atagaatcga aggaagattg ggtcaagatg caggtggttt tgctttctaa ccataaatat
     4561 tatacccagg tggccaatgt gttaagagat gttaatcctg actcccaaat tgaaaatttg
     4621 cttcaaaacc tgcaaaaaaa cacctaaggt ttttgaaaaa cgtttacctt tgatagctca
     4681 atattttagg cttaatggct ggtgatggaa gtgcaaccag ttattttgaa tattgataaa
     4741 atggaagctt ataattttaa tacaatggaa acaatggatt ggaaaaatct gccttttggt
     4801 tatcaaaaga cagattacaa cgttagatgt tactggaggg atggtaactg ggggagactc
     4861 gagatttgtt catcagagtt cattcatatg cacatggcag ctacttgttt gcattatgga
     4921 caagaagcat ttgaagggct taaggcctat cgtggagctg ataaccaaat caggcttttc
     4981 aggcttgaag aaaatgcaaa aaggatggca gagactgccg aatacatttt aatgcaaaaa
     5041 gtgccggttg aattgtttag agatgctgta attaaagctg ttcagttaaa cgaaaggttt
     5101 gtacctccat atggtacggg ggctactttg tatgttcggc ctcttttaat tggaactggt
     5161 gctgaagttg gagttaagcc tgctaaagaa tatctttttt tggtttttgt gactcctgtg
     5221 ggagcttatt ttaaagaagg ctttaaacct acagatatgg taatatgcag agaatatgac
     5281 cgagcagctc ctcttgggac agggcatgta aaagttgggg gaaattatgc cgccagttta
     5341 ctgtcactta aaaaagctca tgatgaaggg tattcgtcgg ccatttatct tgatgccaga
     5401 gagaaaaagt acattgatga aagtggcccg gcaaattttt ttggaataaa agataacaag
     5461 tacattactc ctcgttcgcg ttcaatattg ccatctatta ctaataatag tttgatggtt
     5521 cttgccaaag atatgggaat ggaagtggag caacggcaaa tagaaatatc agaactgagt
     5581 acttttgaag aggcaggagc ttgcggtact gcagctgtta tctcgccaat caggagtatt
     5641 acagaccctc agatggaaaa ggtttatgtt tttagcaaag atggcaaagc aggtccagtt
     5701 tgccagaagc tttattctcg tcttactgca atacaatttg gagatgagcc tgataaattt
     5761 gggtggatca ccattgttga accaacagaa acaaatacca tatctaaata taaaatggaa
     5821 ttgaaagaaa taatgcattc tgttgctgcc aatgttaaga attacgcaaa caaaagggca
     5881 aaaaataaaa cgaaataaat accatgtccg gcaatccaaa taaagatagc tatagaaggc
     5941 tcaggagttc gtatgtaact tctactataa gccttacgct tgtgctttta ttgttgggta
     6001 ttgttggttt gctgatatta aatgctaaaa agctttcaga cgtagtaaag gaaaatatca
     6061 actttacgat agtccttaaa ccaaatgtag aggaagctga tattttgaaa cttcaaaagc
     6121 agatagacct tttgcagtat gttaaatcta cccgatatct aacaaaggaa aaggcagctg
     6181 aagagttttc aaaagatttg ggagaagatt tcgtctcatt tttaggtttc aatcctttac
     6241 tttcaagtat tgaagtgaaa atgcatgctc aatatgctaa tgaacagtat gtaaaactat
     6301 ttgaaaacaa gcttaaaagt tatcctgttg ttagagaagt tatttatgaa aaatcgttaa
     6361 ttcatttggt gaatgaaaat gtcaggaaaa taagtcttgt tattcttaca ttttcggcat
     6421 tgttgttttt gatagctgtt gcccttatta ataatactgt aagactgtta gttcattcga
     6481 ggcgttttac aattcgtact atgcagcttg taggagcaac aaaaagtttt atccgcaaac
     6541 catttttgtt caaaagtatg gtgcttggct ttatggcttc cacaatttcg atagttgcaa
     6601 tttcggggat attgatatat accgagcgta attttagtga tatgagtttg cttgtagatt
     6661 ttaggttgct ctattatctg tttggcggat taattgtatt aggtataatg ataaacctga
     6721 taagcacttt ttttgcatta aataaatatc taaatattca aaaaaacaat ctttattaaa
     6781 aataaactat ggcaaaggca ataaataaaa aagaaagcga agcaaaagtt gattttgctt
     6841 ttggaaaaat aaattacata ttgatggtag taggagttgt gattatcttt attggttatc
     6901 tgcttatgat gggtggaaaa tctgaagatc caaacgtttt taatgctgac gaaatattca
     6961 gttttcgtcg ggttacactt gcacccattg ttgttctttt aggatttgct tttgaaattt
     7021 atgcaattat gaaaaagccc gtggaatagc cacagggctt ttattggatt tttcctttgt
     7081 aatttttttc aaattcttca agggaggtaa atttatattt ttcttcggct ataaaatgta
     7141 gtatagtttc catttgcatg gcatttttat aacccgggat aggcccaagg tattcaaatc
     7201 tttcagtcat ataaacaata gtagggtatg agggccgcca tcctaataat gacaaagcta
     7261 aggggtgagt ccttgagccg aaattaatat atttgctgcc taaaaaagta attgtatctt
     7321 ttgtttctgc attgaacttg acagggtaaa aatacttgtt gagatatttt gcaatttcag
     7381 ggtgttgaaa tgtttcttta tccatcttct tgcaccagcc acaccaatcg gtatatgcat
     7441 ctatcaaaat ctttttggga tttgttttgc atagtgtttc tgcttgttca agagataacc
     7501 acattacttt cgtcgaatcg atagctgcct tttgctgggc taaaccatat aaaggcagag
     7561 ctactgcaat taaaataaag agttttttca tccttgtttg tgtttaacga accatgctcg
     7621 ttgttttatt gataaaattt ttttcaaata tattaaaata aaggacttaa caaccttgcc
     7681 agagattctt tggctgcatt aatccaaggt cggttggacc attcttcaat ggagtaaact
     7741 acgcaagact gtaaatcctt aaggtaatgg gcttccagtc ggtttgtcaa ttcttcatca
     7801 taaatgattg catttacttc aaaattttgg tcaaagctac ggatgtccat gttggctgta
     7861 cctattgagg caactactcc atcaacaagc ataagcttgg agtggttgaa tcctttggca
     7921 tagaaatata tctttacccc ggcttcgagc aaatcggcta caaaagacag ggtgcttctg
     7981 taaactacgt gtgagtcaga tttgtatggg agcatgatgc gaacatctat accgcttagg
     8041 gctgctgttt tcagtgcagt aagtatgctt tcgttaggga taaaatatgg tgtagcaatg
     8101 taaatgtggt cagtagcggt tacgatagcc gaaaaataag cctgcatgat acttgcccaa
     8161 tccgaatcag ggccgctggc agtaatttgg actatctttt tttcgtttat ttggcagggt
     8221 gggaaaaacc tgtcatcgtt gatggcttgg ttggtaacaa agtaccagtc aatgagaaat
     8281 attgtttgta atgaatgcgc agcttctcct tcaatacgaa gctgcatgtc tttccacgac
     8341 ccaagttcgg caaccccttc aatatagcgg tcggcaatat tcattcctcc aacatggcca
     8401 atattcccgt caacaacaat aatttttctg tggttgcggt aattgatttt attagtgaaa
     8461 taaggaaagc gaacaggtaa aaaagcatgt acttcaacac ctgcagcaat taatggttgt
     8521 agaaattttt tgccaagacc ccagctgccc aaatcgtcat aaatcaacct gacttctact
     8581 ccttctttgg ctttactgac aagtaaatca cgaatttggt tacctatttt gtcttcttca
     8641 atgatataat attcgaggtg gatatggctt tttgcatttg ttatgtcgct gataatgatg
     8701 tcaaatgcat ctctgcctga tgtatatatg tgaagttggt tgttttcggt tagcattgct
     8761 ttgctgttat tgagcaaaag cttcattatt tgtgttttgc tttgtaattt ttcgcttaac
     8821 aaagcggcat gtttgtctaa gtgctgcatt tgaatatgac tgaaatcgcg catacgttca
     8881 aagtcgctca gtcctttgcg gttgaaaatt ttttgtcggc gataattttg cccaaaataa
     8941 aaatagagaa tgattccggc aatagggata agaataatca ccaaaagcca tgatattgtt
     9001 ttaagaggtt cgccccgctg cttgattacc aggtaaataa taaacgaaac agtaatagaa
     9061 tatagaatta tgagataatt ttcagccagg aaccgcagca taattacttt ttgtttgggt
     9121 taaaaagctg ctcgcggtac gaaagcatcg ggatcttgat gtaatgggct attttttcgg
     9181 tagcatcaat ttcaagtaag gacttgaaga agccttcgtg ttctttgagc atcaatagga
     9241 tgtcagcgtt gtcagcgtct aaagcgtcta aaatcccttt tacaactggc tttgcttgga
     9301 tttgtttata tgaaaggcat ttacaattta tactttctgc tattttctgc ttgaaacctg
     9361 tcatttttaa ttcggcattg aaatcaatcg gttttggtga aacgtgcaaa atctgtatat
     9421 ctggttgttt gttttttgaa agctttagga aagattgcag aagtgcaatg tctgccttgt
     9481 taaagtcagt agcaaaaaca agtttctcaa tgggtttgta actggcattt gaaggtacta
     9541 ccattaccgg gcacattgat tcggatatta tgcttgatat tgtattgtgt gtaaagcgtg
     9601 tgattaagtc ctgtgacctg gaaacagcta aaatcaacag ttctatttct tcagtagcag
     9661 actctgatat gacttcattg accaggtgcc ctgcaataac ttttttattg aaaggaacag
     9721 aagccggaag agaagggttt atttcctgaa taaggttttc aaaagaggtc atagcagact
     9781 cttccatcat ttttatatta tcagaaggga gtattgatga aataagctct gcttcggaag
     9841 ctccagccgc aaatttgtat atttcaagca catgaagcag ctcaacagac aagcctaagt
     9901 ctttggccaa ctgaaacccg tataaaatag aatttttaga aatgtctgag aaatctgttg
     9961 gtataagtat ctttttcatg gctaatattt ttaaaggtca caattttagc aaaaatatta
    10021 ataaagccta ataaagatac aaaacttcat cagtaaaacc tattttttaa atataaaata
    10081 aacggccaaa atcaagaaaa taaaaccaat caggtggttt aacctgaatg tttcgtttct
    10141 gaacaacagg gtggtaaaaa ttataaatac cgtgagggta attacttcct gtattacttt
    10201 caattcaact aaagtaaaag ggcccccgtt ttctttgtag ccaatacggt tggccggcac
    10261 ctgaaaaaaa tattcaaaga gggcgatgcc ccaacttatc aaaattactg ccagcagaga
    10321 caaagatgaa aaccatttca tttctttgaa tttcaggtgg ccataccacg ccatggtcat
    10381 gaatgcatta gaagtgacca aaagaagtat agaatacaag cctttcatat acaaatactt
    10441 aaaattgacg ggtgcaaaat tatcgatttc attgttttta aaacaactta aatgatgatt
    10501 caaattatct ttgtgcaaaa ttttaacact atggaaggaa agaaagtacg tgtacgattt
    10561 gctcccagtc ctaccgggcc tttacacatt ggaggcgttc gcaccgcgtt atacaattat
    10621 ttgtttgcca aaaaatatga cggagatttt cttttacgta tagaagatac agattcgaac
    10681 cgttttgtgc ccggagcaga agaatacata gtggattcac tcagatggtg cggcattgag
    10741 tttactgaag gtgttggaat tggaggccct catgctccgt acagacagag tgagcgtaaa
    10801 gatatatatt taaagtatgc tctacagctt gttgagagcg gaaatgctta ttatgctttt
    10861 gatacctcag aagagcttga taaccttcgt aaatcgcagg aagctaaagg tatggcgttt
    10921 acttataatt atgaagtacg taccaggctt aaaacatctt taaacccgga atatgcagac
    10981 tatcaggaac gcataaaaaa aggcgaacct tgggttattc gttttaagat gcctgtaaat
    11041 caaaatgtac acatgaatga ccttatcagg ggcgaagtaa ttgtcaatac ttcagcgctt
    11101 gacgataagg tattgtttaa aagcgctgac ggactgccaa catatcacct ggccaatgtt
    11161 acagacgatt acctgatgag aataacccat gtcatcaggg gcgaggagtg gttgccttca
    11221 ttgcctttgc atgtattgct ttacaaatct tttggttggg aatcaaaaat gcctttattt
    11281 gcgcatcttc cgttgctcct taagcctgat ggcaaaggga aacttagcaa acgcgatggc
    11341 gacaggttag gtttccctgt atttcctaaa cattgggatg atccggctac aaaagagctg
    11401 gtaaagggtt acgatgatta tggtttcctt cctcaagcgt ttatcaattt tcttgcttta
    11461 cttggttgga atcctggtac agagcaggaa atatttaata taaatgagtt ggcagatgct
    11521 ttttcattag acaaggttag taaagctggt gctaaatttg attttgaaaa agctaaatgg
    11581 tttaatcatc agtatatcca aaagcttacc aataagcaat tagctgaaat gatatgtagc
    11641 gttttggatg aaaaagggat taaatgcgaa atgtcttatt tagctgatat ttgtgggctt
    11701 gttaaggaga gaatgttttt tctcaatgat ttttgggcca atgcctactt tttcttccag
    11761 tcccctgaag ggtacgatgc tgaggcagta aaaaaacgct ggaaagacaa tgttcccgaa
    11821 ttgttaaaag acgttaaggc tgtgttatcc gatatcagcc attttgaggc aaatagtatc
    11881 aatgatactt tgcatcatca tgttgaagct aagcagattt ctatgggggt attgatgaat
    11941 gctttgcgta tatgcatggt tggggcttct tttggcccag atttgccgtt aattatggaa
    12001 gttgttggca aagaagaaac attacgacga atagacaagg ctttaaacga aataaagtta
    12061 taaatctata caatggtgcg gtttttatat agtgccattt ttgtatttgt tttttcatgt
    12121 gctgttgcac aagaatgtca gatagttgcc tcgggtggaa tggcctacag gctttttgtc
    12181 attcccgaca gtctttcggc tgctgaggct aaacatcaga aagatttaaa accaggtaat
    12241 agctttggtt ttgatgtcat ttttatgtat gagcaaacgg gctggggaat tggctattct
    12301 actttcagag gtagaaaaag tttatataat gaagaaggaa gctctttgac tgctttgcct
    12361 tcgtatgacc aagtggttgt gaattttttc agcctcaatt attatttgcg taaatctgtt
    12421 ttttctgaaa agcttttttt gaatgggaaa atagggcttg ggtatgttgc atataaggat
    12481 tactgccggg agggaggaga agaatatacc aaaacttccg gtacttatgg cataagttca
    12541 tatattgggc tttcatatat ggtttccaaa cattttttgg taggggccaa tttggggctt
    12601 tttatttcta atactgataa aatcaaagtt gatggggtag agcaagcgct ttcatcaaag
    12661 ctgcgtttaa ctcgagtgga gtctaatgtt tgtttaggat acatttttta aaaaagaaaa
    12721 gcggctatta ccgggccgct tttttaaata ctgttaatat aaaccttcta ttgataagta
    12781 tctttcgccg gtgtcatagc tgaaagttaa tactcgactg cctttaggta tttcagggag
    12841 ttttttggca actgcagcaa gcgatgcccc tgatgatact cctacaaaca atccttcttc
    12901 taatgctgct cttctggcgt attcgtatgc ttcgtccttg gtgatgcgga tgaccccgtc
    12961 aagaatattg gtgtgaaggt tctgtggtat aaaccctgcc ccaatgccct gaagtgggtg
    13021 aggacttggc tgtccaccac taataacagg agaagcatca ggctctactg caaaaacctt
    13081 taagtttggg aatttagctt tcaaagtttc ggaaacgccg gtaatatggc ctccagtgcc
    13141 tacacctgtt attaaataat caataccctc aggaaaatcg ttgataattt cctgtgctgt
    13201 tgtgcgttta tgggcttcaa tgttagcttc attgctgaat tggtctggca tccatgcatt
    13261 aggagttgaa gaaacaagtt ctttagcttt ttcgatagct ccacgcattc ctttttcacg
    13321 cggggtaagc tcaaattggg ccccataaac agccattaac cttctgcgtt cgatactgaa
    13381 tgattcgggc atcaccaaga tgagtttgta tttttttacg gcagcaacca aagctaaccc
    13441 aattcctgta ttcccggatg ttggttcaat aatgacgctt tcgggtttaa gtatcccttt
    13501 tttttcggca tcttctacca ttgcaagggc aatacggtct tttattgacg aacccggatt
    13561 ggtcttttct acttttaccc atacttcatg gtttttgcca tacaaactgt taaccctcac
    13621 gtgaggggtg ttaccgatgg tttctaaaat gctttttgct ttcatattta tcacgtttta
    13681 aaattttcag gaagtaattt ctttaacaaa ggtagttttt atttgagaga aaacaaattt
    13741 attttcaact ttttgttgtt ttattttatt gaaaataaat aatataagtg attatcaatg
    13801 ttttatattt ttttaatcat ataggaaaaa taggaataat aacttttaaa aaatattgat
    13861 acagagtatt ggaaaatagt ttactttaca ataaaaaagt cgtgaataaa ataatatata
    13921 tattttctct tttagctttt ttagctgttt cttgtacttc tccggatatt gaagtggttg
    13981 gtattaccga tattagcgta agcgagttgt catttaccca ggtaaaaggc aaagtaggaa
    14041 tagaaattga tagtaaaagt atgctaagct atacaataga tagtagtgac cttgagctgt
    14101 ggttcgataa taacaagctt ggaaagatta cgcttcttga accttttgaa attaaaggta
    14161 acacaagtaa aacatacaat gtaccgttta ttcttaagcc tgaaaatttg aaggctgctg
    14221 ttacggttat tcccaaggtc ttaaaaggcg atcagcctaa agttgccatt aaaggttcgg
    14281 tacaagcaaa aactttgttt ttgaaaaaga agataaaatt tgaatcatta attagtaagt
    14341 aattttttac ctttgcatgg cgaataaaaa tgtatggcaa agtttaaatt gtttataacc
    14401 tgcgttttgt gttttctgat aacacatttt tctttttcgc aatccccaac ttcagatatt
    14461 tttggaattg ttacatcaaa ggcgggtaac gagggaacaa ttaaaatctt acagagtaac
    14521 caaattaaaa cccttgtcaa tgcttatgtt cagagcaagc aaagtgatgg tctcattgtt
    14581 gggtacagaa ttcgtatttt ttccaattct ggccaattgg cccgcggaaa agctgcaagt
    14641 gaacgaagcc gttttatttc gctttaccca gacattaata catatcaaga atacgaggcc
    14701 cctaatttta agatttatgt tggagatttt agaaataaac tggaagcctt taaagcttat
    14761 caaaagatct cgaaagattt taaaagtgcg tttattatat ctacacggat aaatttacca
    14821 aaactataat agaagggaat gcaagatgag tgaattgata aaacatgaat gcggaattgc
    14881 actggtaagg cttctaaaac cgcttgagta ttatcaattt aaatatggga cctggcgtta
    14941 tggattgcaa aagctttatc ttcttatgga gaaacagcat aatcgggggc aggatggagc
    15001 aggggttgta agtcttaaac tcgaccaaaa acctggtact gactatcttc acaggcagcg
    15061 ttctaacgag cctcaggcta ttgaggctat tttcagggac atttatagcc aatattctaa
    15121 tattgaagac cgtttgttgt ctgacgctgt ttgggctaaa caaaacttgc cttttgcagg
    15181 cgaagtgttt ttaggacatc tgcgttatgg gacatttgga ggtagtaaca ttgagtttgt
    15241 ccatccggta atacgtgaaa acaactggcg aaccaggaat cttgctttgg ccgggaactt
    15301 caaccttact aatgttgatg aaattttcaa tcacctgata gagttagggc agcaccctaa
    15361 aggttattct gatactgtga ctattttaga aaacgttggt cactttttag atatggaagt
    15421 acagcgtaaa ttcattttgt tcaagaccca gggatttgaa aacaaagaaa tatctgacct
    15481 tatagctgag catattgatg tggctgctgt attgcgcgag tcaagtaaac gttgggatgg
    15541 agggtatgcc attggtggta tcattgggca tggcgactgt tttgtagccc gcgacccgta
    15601 tggcatcagg ccggctttct attatgctga tgatgaaata gtagttgtgg cttcagagcg
    15661 tacggtgatt caaacagtta tgaatgccga atatgaaaaa gttcatgaaa tagatcccgg
    15721 gaaagcattg attgttaaaa aaagcgggtt tgtttcgata gaagaaataa ggccatcaat
    15781 gcctcgtaag gcttgttcgt ttgaaaggat atatttttcg aggggtagtg ataaagatat
    15841 ttataatgag cgcaaaaagc ttggcgaact tcttacccct gctattctta attcggtcaa
    15901 tggcgatttt gaaaatactg tattttcata tatccccaat actgctgagt cggcttttta
    15961 tggtatggcc aaaggtattg aagatcatat aaagcaatat aaaaaaacaa agattcttga
    16021 gcttggccca aaactaacgc ctgaagctct tgataaaatt atggcattgc gtccccgaat
    16081 agaaaaagtt gcccacaaag acattaagct tcgtactttc attacgcaag ataagggccg
    16141 cgatgactta gtaggccacg tttacgatat tacttatggt acaattaaaa aaggcataga
    16201 taaccttgta attattgacg attcaattgt ccggggcaca acattgaagc agtctatcat
    16261 ccgtatcctt gacaggcttg ggcctaaacg catagttatt gtgtcatctt ctccgcaaat
    16321 cagatatcca gattgttatg gaatagacat ggctaagtta ggcgatttta ttgcattcaa
    16381 agcggcaata gccttactta aagattctgg taaggaacat attatcaacg aagtgtataa
    16441 aaaagctaaa ttacaacagg gattgcccaa agaagaaatt gtcaaccatg taaagagcat
    16501 ttatgagcct tttacggatg agcagatttc tgataaaata gcagaactcc taacatcttc
    16561 agatatcaaa gccgagataa agattgttta tcaaactgtg gagaatttgc acagagcttg
    16621 tccaaaccat gttggagact ggtattttac cggggattat ccaacaccag gaggaaacaa
    16681 ggttgtaaat acgtctttta ttaattttat tgaaggtaaa aaccagcgtg catactaaca
    16741 tgcaacgttt tgttgaaaaa ctcgaagccc ggggcgaact catcaggata aaagagtttg
    16801 ttgatccaat tcttgaaatt gctgaattta ccgatcgtat ttcaaagatg cctggtgggg
    16861 gtaaagcgat attgtttgag aatacaggta cggcctttcc tgtgctcacc aatgcaatgg
    16921 gttctgataa gcgtatttgc gaagctcttc atactaaatc attagatgag attggccata
    16981 gaattgataa gctgtttcac attttttcaa gccccaaaaa taccatttgg caaaagctaa
    17041 gtctgttgcc tcagcttaaa gaagtaagta attggatgcc cagaaaaaaa agaggcagag
    17101 gcgtttgcca acaggcagtg atgcaagaac ctgatttgtc aaagctccca attctcaaat
    17161 gttggcctgg cgatggtggt cgatttgtaa cattgccatt ggttaatact attgaccccg
    17221 atagtggtat cagaaatata gggatgtacc gtatgcaggt ttttgcaaaa gacaaaaccg
    17281 ggatgcattg gcatatgcac aaaaccgggg ctcgtcatta cgaagcttat aaaaaagctg
    17341 gggaaaaaat gccggtaact gttgtacttg gcggagaccc tgctattatt tatgccgcaa
    17401 ctgctccact tcctgataat attgacgaat atattcttgc cgggtttctt caaaataagc
    17461 ctgtagagtt ggtaaaatgc ttgacaaacg aactctgggt gccttcagag gcagacatta
    17521 tcatcgaagg ctatgttgac cccaaggaag aaaaactttg ggaagggcct tttggcgatc
    17581 ataccgggtt ttattctgag cctgactggt atccggcatt tcatgtaaca tgtataactc
    17641 atcgtcgtga tgccatatat cctgcaacta ttgtaggtgt ccctccgcaa gaagatgctt
    17701 atattgcaaa agctacagaa cggatatttt tgtcacctat gcgatttgcc cttgctccgg
    17761 aggttgaaga tatatacctg ccagcagaag gggttgctca caatttagct attgttaaaa
    17821 tcaataagac ataccccggc caggctttca aagtgatgaa tgctttatgg ggcgcaggtc
    17881 agatgatgtt taacaaaacc atgattgtta ctgacggcaa tgttgatata tttaatccaa
    17941 taagtgtatt agaagcttgt gctgaaaatg tttttcagac acaccatatc tgttttcagg
    18001 aaggaccggc tgatattctt gaccatgcag gtaaggcatt tggctttagc ggaaaaatgt
    18061 gtattgacgc tacccgaaaa atgccacagg aaatcatagc tccgtttgac aataatgttt
    18121 attctgaaaa agaggcagaa atattcattg atcaaataaa taatattgaa aatatatgca
    18181 gtgccaacag tttaaaaaca aaaaatggaa aaacccttgt gtttgctggt tttaaaaaag
    18241 aaacccccgg agatgtaaga acgtttatca gtaaattaaa aaacagtaca tatttaccat
    18301 ctcttttggg tatcattctt tttgataaaa atactgttct gggccatttt agttatttgt
    18361 catggttgac tttgtcaaat attgatgcta caagagattg ttttgtagtt gaacatcaaa
    18421 gaggaataac acttattatt gatgctacct caaagtcact tgaaaaggat aacttcaaca
    18481 ggcaatggcc tgcaataatc atgtcatcag atgaaaccat aaaaaatatt gacaaaaaat
    18541 ggtcaatcct tacaaagctt ccttttgttc attcaccgac tttattgtcg aaaaaacaat
    18601 caggaataca ggaataaatg acaaatatta atttttgtta aaaccttttt ggtaatcatt
    18661 aaaagaaatg ctattttagc cattgatgag ggttgtattt gcaatattga ccatacttct
    18721 cttttttgag aatacatctt tttcggccaa cagggtgtta gggtcgcgaa cagagatgta
    18781 caccattgag aacgggctgt ctcaatctta tgtcaaccat ctttttattg attctcgtgg
    18841 ttttttatgg atttcaactt atgacgggct taaccgtttt gacgggctca attttgtggt
    18901 ctataagcat aatcctgttg atacaaattc attgagtgac aattacgtgc gccatgtatg
    18961 cgaagacagg gatggcaata tatgggtagc taccaacaat ggccttagct gttatctttt
    19021 ttcaaaaggt gtatttgtaa attattacaa cgatcctcaa tatgcttcat ctatttcttc
    19081 aaacagcata tttaatgttt tttgtgataa aaatgatgtt atttgggcca aaactctaac
    19141 ttcacttgaa aaatttgata aaaagcagca aaagttttat cattattatc atcataatga
    19201 tatttttaac caaattattg atgaccgtaa ctttgacatt tttgaagatt cacgcgaaaa
    19261 actatggata gccacaaaag atgggttgag ttgttttgat aaaaggttag agctatttga
    19321 acattttaca tcaactccat ctgatataaa aactattagc gacaatagga tcaggagtat
    19381 atgtgaagat tgcttcggca atatttgggt gggcactgcc aacggattaa acaagtttga
    19441 tataaaacaa aggcgttttt acagaattta tgccggctct gataatgttt cattgattag
    19501 caataaaata agttctttat atacagcaga taagaaaaag atttttgttg gaacagagac
    19561 cgggctttca attttgaata ttaacagtat gacatttgcc aattatcctt tttttaatat
    19621 tcaaaatagc gaggtcccct tatccaacat aaaatcattg ttgctcgata aagctaataa
    19681 cctttggatt ggctgtcgtg aaggacttat taaagttgat ttgaagcctc ttaagtttgg
    19741 tttgtttcgg tcttcaggcg gggcttttag cgagaatatc agttctattt atgctcaaag
    19801 caaaaacgat atatggttag gaacttggaa taatgggcta cttagggtta atctgggggc
    19861 agaagaacat tcctcttctg ttaagccggc aaaaagatac tttagtaata catcaatata
    19921 ctcattatat aaagataaaa ataataaact ttggatttcg actgaaaacg ggcttactat
    19981 atttaacata aaaactaata cttttgagcc ttttaagcct aaaaacaaaa tgccttatgg
    20041 ttttagcaac gaaaaaatat attgttttta cggcgatggt gacaaaattt ggatgggaac
    20101 aaaaatgggc ttattgctat ttgataataa attcaatgag gctcatttgt tttcaaaaat
    20161 acctgacagt acaggaatac aagtaaattc tgcatattgc cttacaaaag acaagtcagg
    20221 ggctctttgg ataggcacca atgacggcct gatttgtttt aatcccgaaa caggcgaata
    20281 caaaaaatac ctttcaagtc ctcattacac tcaaaatgcc ctgtgcagca acactatata
    20341 ttcattgtgt tttacatcca aaggagaact ttggataggg accaacgccg gattgtgtaa
    20401 atacaatgaa gaaaaggaca attttattat ctattcagaa gtagatggtt tgcctaataa
    20461 taccatttat gctatattag aagatcagaa taataacctt tggttaagca gcaataaggg
    20521 aataattctt ttagagctgc cattggggtc atttacttca tttgatcagg cagatggctt
    20581 gcaaagtttt gagtttaacc atgcagcagc ttataaagac aatactggat gcctttattt
    20641 tggcggcatc aatgggttaa attattttta tcccgattca ttaaaaccca acaaacacat
    20701 tccttcggtt cagatcaccc ttgttgaaat aattggcaat agtatcagga gaataagcgc
    20761 tcttagtgat ggaaagctta tctctatccc tgccaatacc aaacttttca ctattaattt
    20821 tgcttcgtta gattttacat ttccttcaaa taatcaatac atgtacaagc tttcttcaaa
    20881 aaaaagcgaa ggcatgtgga taaataatgg aacaaagaat tttgttcaat actcaaactt
    20941 gcctgagggg cggtattatt tcagggtaaa agggtcaaac agcgataagg tctggagtat
    21001 caatgaatat tcacttattt tacaggttga agccccctgg tacaaaagaa agattgccta
    21061 tttcttttat tttctgcttt ctgcattatt ggtttatggt acggtgcaac tgcgtacgcg
    21121 caaacttatc aaaaccaata aagaacttat tgataaagaa aagatagctc agaaaattgc
    21181 ccgacaaaaa gaagagctta ccttgaaaaa caagagcatc acagatagta taaattatgc
    21241 taagcgtatt caggaggcta tgatgccttc agataaatat ctgaaacgca tattgcccaa
    21301 ttcattttta tttcataaac ccaaagacat tgttagtggc gattttgcct gggttagcga
    21361 acgcgatggt aaagtattgg ttgcagctgt tgattgtaca ggtcatggtg ttcctggagc
    21421 ttttatgtct atcttgggga tagagctttt tagaaatatt ccaaacctgt catggaaatc
    21481 gccgggggcc atattgaagc aaataaacga tgagtttgtg cacattttta gcgacatgga
    21541 agatgttagc ctccgcgatg gtatggatgt agctctttgt attattgata agaaaaatta
    21601 tacagtacat tttgccggtg cggtaaatcc gctttatctt gttacggggg acaaactgga
    21661 agaaataaaa gggagccgtt tttctatcag tattgaaaag cgcactgaag agatattttt
    21721 tgaagaccat atggtacagt tgaaaaaagg ggatatgata tacatattct ctgatggtta
    21781 tgctgatcag tttgggggat acgaaggtaa aaaatttaaa ttcagaaggt ttaggcattt
    21841 gcttcttaat atttcgcagt tgccgcttga tagtcagaag gaatatctgc ttgaatcaat
    21901 tgaaaattgg cgtggagcgt acgaacaggt agatgatttg cttgttattg gcattagagc
    21961 agattttgaa tgaatagtat ttaacccaaa ttaagcatta ggaggctttt taaagccgaa
    22021 ctatatgatt ttagtcgggt aatcttctcg ttataaacag gacaagctga tttagaaatt
    22081 gtttgaagaa ttttattaaa ttattgaatt acaatttctt aatcgattaa gcatttttaa
    22141 tgcttaattt gggtcaaact tttttgatta gagctttaaa tctattgtta gtttttaagc
    22201 aattccttca tttttgaaac cagtacatcc agctggaatg gtttgctgat gtattcgttc
    22261 attccggcag aaaggcatat ttcgcggtcg cccgacaaag cattggcggt gatagcaata
    22321 ataggtacat gagagttagt gctggcttcg agttcgcgta tcttacgggt agcagtaagc
    22381 ccgtccataa ctggcatttg tatatccatc agtacgatgt catatttttt attgccaaac
    22441 atatctaagg cttcttttcc attattggcc acatcgatat tggctactaa tgttttaagg
    22501 ctcaggagaa caatcttttg gttgattaag ttatcttcga ccaacaaaac attggcttct
    22561 tttaaatcaa cgtttttcac tgtcgagcta actgcagaaa caattgttgg tgcagccgtc
    22621 ggagcttcag aagcagattt tttatactta atgctaaatt ctatagtggt aattccgtta
    22681 gaacaactgg ctacaagcct gctgcccaat acctctatca attttttaga gatagaaagg
    22741 tcaaaaccag gctcgacaat agacaatttg ttgtgcatgt catcactaaa cagccagctt
    22801 tcttctgtaa ggtttactac agggagtaag aaatcaaaat ttatctgaaa attaaattcc
    22861 acctggctgg aattttcttt taccagctca aggtttattt ttacatggtt gccttctggt
    22921 tttctaaatt tcaggatgtt ttcaatcagg ttcagaaata tctgtttaat cctgattgga
    22981 tcgcccataa cgttgtaacg tttgtctttg ccttcaagct caaatgcaac aacttgctga
    23041 tattgctgtt cgaaaagttt taaactgctt tgaatagtgg ataaaagctc aaagttgatt
    23101 ttattttctt tttcagtaat ttctgccgca gaaactttaa caatactatt gacaacatta
    23161 acaaggttat gggttgaagc taacactgat tctaacaaat cccgttggtt atcatcaagc
    23221 tttgtttctg aaagcaaatc gcccaaaacc ataatattat tgagaggtgt acggatttgg
    23281 tgcgacagtt tgcttaaaaa ttcatccttg ctccttgatt cgtctttggc ttctttgatg
    23341 tttgtttcgg ctaccgtaaa gctttgttct gtaaggtata aatatgcata agtgaggaca
    23401 gatacaaaga aataaatcat gattatcctt actataagag gaaaagtgta atgcacttta
    23461 agcaaacctg caggctgaat aacaagaatt aatgtaataa tcagtaaaaa gcccatagca
    23521 aaaagaaccc ctttgcgtgt gccatacaaa aacatactga taatagggta agaaaagatc
    23581 cacaaatagc cataatctcc aactcctccg gaaataacaa aataaatgta cactaccgaa
    23641 gtaagtatca ttaaaaaatg gttggaaata atgtaattct ttgttttttg caggataata
    23701 aagttagcag ctaccataat ggtaactatt gccactatga tagcatgaaa ataattctcg
    23761 tatgaaacag agataaatat aaaggagaat aataataaca atgagagaaa cgtaaacaaa
    23821 ttagtgatta caattgagta acgcccctgt ttattggtaa caccttgtgt cccaattgac
    23881 aatttttcaa atatccattg agaaaaagac ataattagat actttgtttt tttacgaaaa
    23941 tattaaaaaa aaacatttaa tcaagaataa tcatcaaagt tattcgtgca ttcatggaat
    24001 atatttttat taatttttat aattttaaag caaaatggac gtttttctaa ttgtttaaaa
    24061 ttcaacatgt taaattaata ctaataaaac catgaaaaaa ttatttttcg ttcttattat
    24121 tgctttggct acacttactt cattttcgcc agattatgta cctactgctg atgaggtgaa
    24181 caaattttac caaacgaaga ctttggtggt gcttgatgat aatcctcttg ccgaatacaa
    24241 ttttgtgatt aaggatttta tgcaaaagga atggaaactt acgcagtttg attttatcaa
    24301 gcaaagtgag tttgaggaaa agcgaatgga caaacaatat tcgtttatcc ttctgaactc
    24361 ggtacgtttt gataaagata agagccttgc caggtatatt tttttgagcc ttgttcttgg
    24421 aggcgacgat aaaattgttt caaacatgcc agacctttgt cctgtaccta ttgcttatta
    24481 tggggtagat gaagaccatt acaactataa gtttcaaacc atcctgcgtt ttatgcaaca
    24541 gcatgttgag cttattcaca aaaatccaaa gctgataaaa accaacgctt tgaagatcta
    24601 taacgatagc atgcaaagcg tgaaaacaaa gactttgtat ttgattaaag acgagcttgc
    24661 cccagaatgc aatactgaag caaaaatcaa aaaaatttac ccctataagt tcaaaattgt
    24721 gtcacgtgaa gaagtagcaa aggctattga tgagcgaaat cctgatgtag tgtttttgca
    24781 taaagttggc cctgaaggga caaggttaat tgcccgttgt tacaaaatac ttatcggggc
    24841 tgccgattca aagttttatt actttgatta tcataaaatt gaagatgatg ccccggatgg
    24901 gtttttagca aaagatttca agaaattagc aaaatagttt atactttatg taagtataat
    24961 aggccttcca ggattcaaaa acttgggagg ccttgttttt ttaaaacctc ttttgataac
    25021 catgacaata tggcaatgta cctttatggt tgtaataatc ctggtgataa tcttctgcta
    25081 tataaaactc agttgcagga gtgaggccgg ttaccacatc aaccccttta gctttaagca
    25141 tctgaattag ttttagggaa atctctttct gcttttcatt ggtataaaat atttctgagc
    25201 ggtattggtc ccctatatca gggccttgcc tgttcatctg ggtaggatcg tgtatctcga
    25261 aaaacaatcg ggctaattgt tcaaagctag tctttttgtt attaaaaact acctttacca
    25321 cttcggcatg ccctgtagta tgcgtacaaa cgtctttgta tgaagggttg cgggttttac
    25381 cacccataaa accaacatct gtggatataa tgccctcagc tttttgcata taatattcta
    25441 caccccaaaa acagcctcct gcaaagtatg ttgtctcaat agaatctaca ttttctaatg
    25501 gtaaaaactg aatagacaag gaattgacac aatgccttgt atttttgggg gtaaaacctt
    25561 cgccttcaaa aacgtggcct aaatgggcac cacaattgga acatacaatc tcagtccttt
    25621 tgccatctgc gtcagggacc cgttttatgg caccttgtat ttcatcgtca aaagaaggcc
    25681 agccgcaatg tgaatcaaac ttgtcagacg atttgtaaag ctctgcatta cagcgtttgc
    25741 aatggtatgt cccctttttg tcaaaattat aatactttcc gctaaagggg gcttctgtgc
    25801 ctttatacaa gattattctt tcttcttcgg gtgttaatgg attgtaattc atattattat
    25861 ttttttgggc acatgaaagt accggaatta aaaacaacac aaaccacatt ttttgcatca
    25921 ttatattttt ttacataaac aagcaataag taagtttgtt agggcaaagt aaactttatt
    25981 ttatacttcg tcattatttg aaattggtgt aaatttgcgt tcatttttta gaaaagtaaa
    26041 agcacaaaat atgagcgacg acaaaaagat tattttctcg atggtaggtg taggaaagac
    26101 tttcccgcct cacaaaaggg ttttaaacaa tatttatctt tccttctttt atggtgcaaa
    26161 gattggcatc attgggctca atggctctgg caagtccaca ctgctaaaaa ttatagccgg
    26221 agtagaaaaa gcatacgaag gcgaagtagt tttttcacca ggatatactg ttggatatct
    26281 cgaacaggaa cctaaacttg acgattctaa gacggttaaa gaaattgtac aggaaggcgt
    26341 tcaaaaagta gttgatttac ttaaagaata cgaagaagtt aataacaaat ttgctgaccc
    26401 agacttaagt gcagacgata tggataagct tattgcccgt cagggtgagc ttaccgactt
    26461 aatagagcaa catgatgcct ggaacctcga caataaactt gaggttgcta tggacgctct
    26521 tcgttgccct gatgccgaca ctaatgttaa gaacctttcg ggaggagaaa gacgacgtgt
    26581 agctttgtgc cgcctacttt tgcaagagcc cgatatcctg ctccttgatg agcctacaaa
    26641 ccacctcgat gcagaaagcg ttgaatggtt ggaacaacac ttacaacaat ataaaggtac
    26701 tgttattgct attacacacg acaggtactt cctggacaac gttgctggtt ggatacttga
    26761 gctcgaccgt ggcgaaggaa taccatatca gggcaattat acatcatggt tggaacaaaa
    26821 atcgaaacgc ttagaacaag aagaaaaaca agaaagcaaa cgtcgtaaaa ccttagagca
    26881 tgagctcgaa tgggtacgca tgtcgcctaa agcacgtcag gccaaaggta aagcccgttt
    26941 gaacgcgtat gaaaaattga tgagcgaaga taccaagcaa aaagaagaac gtctggaaat
    27001 ttttatcccc aatggcccgc gtttaggcaa caatgttatt gaagctaaga acgtttccaa
    27061 agcatatggt gataagttgt tgtttgaaga cctgaacttt aacctgccgc ctgctggtat
    27121 tgttggcgtt attggcccta acggtgctgg taaaacaaca ttgttcaggc taattatggg
    27181 gcacgacaag cctgattctg gcagtttcag tgttggcgaa actgtaaaag tggcttatat
    27241 tgatcaaagc cacactgcta ttgatccgga aaaaacagtt tttgaggtta tctctggcgg
    27301 tcttgacttt attactatgg gtaataaaca agttaatgcc agggcttatg ttgcaaggtt
    27361 caatttctca ggtgctgacc aggaaaagaa atgcggcgtg ttatccggtg gagagcgcaa
    27421 caggcttcat cttgccctta cactgaaaga agaagccaac gttctgctgc tcgacgagcc
    27481 taccaacgac attgacgtta atacacttcg agctttggaa gaaggtttgg aaaactttgc
    27541 tggctgtgct gtggtcatct cgcacgatcg ctggttcctt gaccgtgttg caactcacat
    27601 cttggcattt gagggcgatt cacaagtgta tttctttgaa ggctcattca gcgaatacga
    27661 agaaaacaaa aagaaacgcc ttggcgatac agggccacac aggattaagt acaaaaaact
    27721 aactcgatag tcagcaaatt tgaaaatttg ccaattagca aatgaatgaa aacaatttta
    27781 aaatgaacca atcttcggaa aatccaatat tgaaattgac ttttgcgttt gctattgagg
    27841 cggtaaaata tagtgaggtt cttgagacca ataaaaaata tgtaaaagca attcttaagg
    27901 tcagcaacat ccataggagc ttgtgttcgg gaagcccaaa atgcagaaag taaattggac
    27961 tttgttcaca aaatgaaaat agctgcaaag gaagctgatg aaaccgagta ttggttagat
    28021 atttgcaagc attcggggca ctatccagaa tgtcaggaat tactggacaa gattaatgaa
    28081 ataaaaaagg tgttaaacaa aataattgga acaactaaga aaagcgtaat tggatttcag
    28141 aaataatgaa tttcatttgc taattctcca attttcaaat tttcaaattg agcatgaaac
    28201 cgagcatacc acaaggtacc cgcgattttg ggcctgtaga aatggcccgc aggaactata
    28261 tatacaatac catccgcaat gtatttgaac tttacgggtt ccagcaaata gaaactcctg
    28321 ccatggaaaa cctttctacc ctattaggaa agtatggcga agaaggcgac aagctgctgt
    28381 ttcggattct aaactcgggc gattacctga cgaaagtgga tgacaaagat ttcagaaaag
    28441 acaattcgct tggattatcc ggaaaaatct gcgaacgagg cttacgttat gaccttactg
    28501 tgccttttgc ccgctttgtt gttcagcacc gcgaagaact gaccttccct ttcaggcgat
    28561 atcagataca gcctgtttgg agagccgaca ggccgcagaa gggaagatac agggaatttt
    28621 atcaatgcga tgcagatatt gttggttcag acagtcttct caatgagtgg gaacttgtac
    28681 agattgttaa taccgtattt gaaaaacttg gcattaacat ttgcctgaaa ataaacaacc
    28741 gcaaagtact tagtggcatt gccgaagcta ttggcgctga gtcccatatt acagacatca
    28801 cggtagccat tgacaagctt gataaaattg gcattgagaa agtaaaagac gaacttaaag
    28861 aacgtggaat tacagaaagc gcagttgaaa agctgatacc tatcattgaa tttaaaggta
    28921 gcagtcggga taagcttgaa ttgctccgta aaacacttgc tggttctgct acgggactta
    28981 aaggtattga agaagtagaa actttactta actatatcga tggggcacag ttgagctgcg
    29041 agatagaatt tgacctttca ttggcccgtg ggctcaatta ttacactgga gccatttttg
    29101 aagtaaaagc caaagatgtt gctattggca gcatctcggg aggcggccgc tacgataacc
    29161 ttacaggagt gtttggtatg cctggggttt caggtgttgg tatttcgttt ggtgctgaca
    29221 gaatctatga tgtattattg cagcttaacc tttttccctc agaaacaagc caaacaacac
    29281 gtattatgtt tgtaaacttt ggcaaaaatg aagaaaacta ttgcttgcca ttggctgcac
    29341 aactgcgtca aaatgggata agttgcgaga tttatcctga tgcccaaaaa ctcaagaaac
    29401 agtttgacta tgccaacaag aaaaatattc aatttgttgt tattgcgggt gaggacgaaa
    29461 taaaagctga cgaaatcact gtgaaaaata tgattacagg agaacaaaca aaaatcagca
    29521 aagaggggat tgtaagtttt atttgtaaaa atatctaaag aaagttattg gttttgaccg
    29581 gtttagcatg atttcggcta tttaacaggt aaagtaaata taaaagaact gcctttgcct
    29641 gcctggcttt ttacataaat tgagccacca tttttttcca caaagtcttt acacaaaact
    29701 aaacctaacc cagttccttt ttcaccttct gtacctttag tactttttgt agaagaaaca
    29761 gaaaatatat ccttaagctt ttcttcttca atgccaatcc cgttgtcttg tatggttatt
    29821 tctatcattc cgtctgaggt tttgtcaaac aattcgatgc gtccactttg aggggtgtat
    29881 tttatggcat ttgaaattaa gttccgaaaa acagtgagga tcattttttc gtcaaaagtt
    29941 gatttggcct ttggtttaat attattaatt atctggatat tttttttgtc agcaatcaac
    30001 ttcaaatggt taacagcaac ttgcgaaaca tcaaatattg ataattcctg acattgaagg
    30061 ccaaatatcc ctttttgaaa tgcagcccac tctaaaagat tgttgaccaa tgaatataag
    30121 ttgtttattg atgaagcaaa ttcgttgagc aaagctgttt tttcggcaag tacggttgtg
    30181 cctgaaatag tgttaattaa agaaactaaa gcagccaaag ggcctcgaag gtcatgggca
    30241 ataattgaaa gcattaggct tttattttca ttaagttgca ataactgcgc gttttttgac
    30301 tctaattcat tttgaatttt cttttgagct gaaatgtcaa atacaatacc aactaattca
    30361 gccggcttgt tgtttgcatc acgtttaaca ataatgccac ggtcataaaa ccacttccac
    30421 tcaccgcttt tgctctttat ccggtattcg cattcataag cttcactaac gccatataag
    30481 tggttacgca tgttttgcat gaccatctca taatcgtcag ggtggatttt tgaagtaaaa
    30541 aattcaaacc caattttgtc cgggatttct tgtacactat atcctaaagc ttctaccttt
    30601 tttgaattaa aatggacttt gttgtcttta acgttccaat gccattttcc aaggttcccg
    30661 ctccattcaa actgagcctc ttcatgatat accaattctt tgttcaattg aaccacctga
    30721 ttttccaggt ccattatttt ctcaataagc tcttcttttg ataagctgtt taaaagggtt
    30781 tcttccataa gatttttgtt ttattatccc aaacaaattg gaaaatgtat tctgtaaaaa
    30841 actaggctaa taaacaatgt gaaaaacgat tcttcaagtg tgctctaatc aaagataact
    30901 aaaaaatatg gcagaaaatg actaattttg aaaaaacaat atttatggaa cggttaataa
    30961 atttgcatat agagaaactt cctgaaggat attatctggc gacttcagat agtattcaag
    31021 gacttatagc tcaaggaagg acgatatctg agactattga gatagctaag gatgttgcta
    31081 aaaaattaat cgaagcgcat gaagatcaaa tttctcaact ggctaaagta aaagactctt
    31141 tcgattatcc tcttgttatt ggggtctgat atgggaaggc tatcaggatt cagatatagg
    31201 gatattattc aaatactcaa gaaatttgga tttgaatttt acaggcaagc tgccggaagc
    31261 catgaattat ggcacaatcc tgttacaaaa aggtttacaa caatacccaa tcatccggga
    31321 gatatgcccg agggtacttt gagtgctatt ctaaaacgac attgacgttg atagtttttt
    31381 aaagataaag taaaaagagt tataaaacta agaagttgaa gaaagctgct aagaaagacg
    31441 aatttgacga taccggcctt gaagtaatag gtattgcaac atctctaaaa gatgtaaccc
    31501 ttctctggca gctcaacaat acattacatt tcaagtttaa gtattttggg gctatagccc
    31561 ttgatgacaa aaatacagat gcatttccat atttcataga taatgaatca aggcagtttc
    31621 aatatgcttt gttttataat aggtataaag cttatacttt ggtgccggca tttaagcaaa
    31681 tagacttctt tttggttagt tacgtaaagc tttatgaaga agaaaaagct gtattgttga
    31741 aagaccttaa atcgatttca ggagtttcca tagctatgca tctttcagaa cctaaaaaga
    31801 atgcatttga gattttttat atcctttaaa aatttttatc cccccttttt ttaactcgct
    31861 gaaaattaaa ttttaaaagc gtaaaagcta cgttttatca ggtttttagc caaataattt
    31921 tcgtcaatat tttaaaattt ggatgaaaaa aacagctttt tttaattaac tttaatatca
    31981 ccttattcat aactaaaaca accctaaata tgaaaagaga attatcaaaa aatcaaaaac
    32041 ctgaagagag cttcgctttt tcaaaccaaa agcgttcatc gttcaatcat gtctatcaag
    32101 catcaatcaa cagttttgat gaagattatt tttttgaggt ggaggatttt taactttgat
    32161 gtacccggta tcagaacaac tgctatggaa cgatggcagt tgtttttatt ttaagcggct
    32221 tccatttttc gaaaaacatt catcaaaata cacaaacttt tttttcagca atacaaaagt
    32281 ttctattaac aattgtcgcc acacctgttt ataaatacga cttctaatgc cccccttctg
    32341 acgttaatac actcaatttt aatttttacc tttgtagcaa aataaaaatc attcatcatg
    32401 acagggctca aaaaaattaa atctgcgtta atttcggtct atcacaaaaa ccatcttgac
    32461 gaaattgtaa agaaactgca tgctcaaggt gtaaaactat attctaccgg aggtacagaa
    32521 gacttcatca aacagcttaa tatacctgtt acagctgttg aatcattgac cggatatcct
    32581 tccattcttg gaggacgcgt aaaaacttta catccaaagg tgtttggagg tattttggcc
    32641 agaaggaatg aaaaaggcga cttgcaacag cttgcagaat acgaaattcc tgaaattgac
    32701 ctggtaattg ttgacctcta tccttttgaa gcaaccgtgg ccagtggagc tgaacatcag
    32761 gctatcattg aaaaaattga cattggaggg atctcgctta tcagggctgc tgccaaaaat
    32821 tatgcagatg tgcttattgt ggcatcacag gctcagtaca aagaccttct tgatatcctc
    32881 aatgacaaag acggacaaag cactttatca gacagaaaaa tgtttgctgc tgaggctttc
    32941 aatatttcgt cacattatga cactgctatt ttcaattatt tcaacaaaga gtacaattca
    33001 aacgtcctta aagtaagtat ccaggaaagc atgcctttgc gttatggcga gaatcctcac
    33061 caaaccggcg ttttctttgg cgatttcaat aaaatgtttg aacaaatttg tggtaaagag
    33121 atttcttata ataatctgct agacattgac gctgctgtaa acctcatcaa tgagtttagt
    33181 gaacctactt ttgccatttt aaaacacaac aatgcttgcg gggttgcttc gcgcagcaat
    33241 atgctcgatg catggaagga tgcacttgcc ggcgacccgg tttcagcttt tgggggcatt
    33301 atcattacta acaggcctat tacaaaagaa gttgcagagg gtatcaatac aatattcttt
    33361 gaagtggtga ttgctcctgc ttatgatgct gcagcattag aggttttaca attaaagaaa
    33421 aaccgtatca tccttatcat aaaagaaacc actcttccta caaaacaaat caggactgtg
    33481 cttaacggtg ttttgatgca ggacaaggac ttaaaatcag aaaacgaaag cgacatgcgg
    33541 actgtaaccc agaaagcgcc tacggcacaa gaaatttctg acctcgaatt tgcatgcaaa
    33601 gtttgtaaac ataccaaatc aaataccatc gttctggcca aaaataaaca actcctggca
    33661 agtggtgttg gacagacttc acgtgtagat gcattgaagc aagctattgt caaggccgga
    33721 agctttggtt ttgacctgaa aggcgctgta atggcttcag atgctttttt cccttttgca
    33781 gactgcgttg aaattgcaag tcaggctggt attacagcta taattcagcc tggaggctct
    33841 gtacgtgaca aagattctat agattattgt gacaataaca acctggctat ggtatttacc
    33901 ggaataaggc actttaaaca ttaaaattta aacatcaaca aaaacgggaa taacaaaaag
    33961 ttcccgtttt tgttttcagg agcccttact caaagctttg gttattgaga tttttaaggg
    34021 ttgtaaaata ctgagatgaa taccagttac taaaagaatt aaatatttga caaaaatagt
    34081 tatcaacata tccattttat gcctcaaata tttataaatt agcagccaat cataagttgg
    34141 tgtcgtgaga catcaaaaaa caatgtaaca aaaaataaaa attgctgata tgggtgtatt
    34201 ttcgttcctt acacaggaaa ttgcaatgga tttgggaacc gccaacacaa ttataatcca
    34261 gaatgacaaa atagtagttg atgaaccttc tatagtggct atcgatacaa aaacagggaa
    34321 attgattgcc attggcgaaa cagcccgcca gatgcacggg aaaacccatg aaaacatcaa
    34381 aaccatcagg ccgttgaaag atggtgttat tgccgatttt aatgctgccg agcaaatgat
    34441 aagggggatg attaagctga taaacaaaaa aaaccagctt ttcaacccgg cattgaaaat
    34501 ggtagttagt atccctttgg gaagtacaga cgttgaaatg cgtgcagtgc gtgactctgc
    34561 cgaacatgcc aacggtcgtg aggtatatat gatatacgag cctatggctg cagcccttgg
    34621 tatcggtatt gacgtagaag caccagaagg aagcatggtt gttgatatcg gagggggaac
    34681 aacagaaatt gccgttattg cccttggtgg tattgtttgc aaccaaagta tccgtattgc
    34741 gggtgatggc ttcacggccg acattcaggc atatatgcgc catcaacata atattaaagt
    34801 aggtgaccgt actgctgagg aaatcaagat tggcgtaggt tctgcattac cagaccttga
    34861 cactccccct gctgactttg ttgtacgagg ccctaacctt atgacagcta tgcctattga
    34921 aatacctgtg tcataccagg aaatatcaca ctgtctggac aaatcattaa gcaaaataga
    34981 agctgctttg gttgctgtgc ttgaacaaac tcctcctgag ctttatgctg atattgttac
    35041 aaaaggtatt ttccttactg gtggaggagc tcttttaaga ggccttgaca aacggttgag
    35101 cgacaggatc cagataccat ttcatgttcc tgaagatccg ttgcacgcag tagccagagg
    35161 tacaggtatt gcgcttaaaa atattgacaa attttcattt ttgatgcgtt aataaagtta
    35221 ttatccagag gcaggtttat atgaatgcta ttatacgctt tattattaag catcagtttg
    35281 tattgttgtt tctgcttctg gaaattatag cattagtgct ttttgtgcaa aataatggat
    35341 atcagaaaat aaagtaccag gcttttgccc aggactttga tggctatttg atgcacaaac
    35401 gtcacagaat tgggcagtat tttggtttaa aagaagtaaa taataagttg tctgctgaaa
    35461 atctgatgct gcgcaggcaa atagaaatgc ttaaagcaga gaaagcaaac aaagataccg
    35521 ctgtgaattt taccagttac ataggggcca gagtgataga caacagtgtt aacaagcctt
    35581 ataattatgt tcttgttgat aaaggggcga aagatggtgt aaagcccgaa atggctgtta
    35641 tttcaaatca gggagtagta ggtttggtag catcagtaag taaaaattat tcattggtag
    35701 cttctgtatt gaaccgcaaa ataaaagtca gtgcaaagat taagaaaaat ggttattttg
    35761 gttcgtttga atgggacggg ttaaattacc gatatgccta tctgcacgat atcccttatc
    35821 atgctgaagt taaagctggc gacaccattg ttacaagtgg ttattcgttg atttttcctg
    35881 aaaatatccc ggttggggtg attgatgaag tggattcaac cgggggcaat tttttgaata
    35941 ttcggacaaa actatcggtt gactataaaa agcttgattt tgtatatatc attgagaata
    36001 attcaagggc tgagatagaa ggattacaga aaaagggaag caatgattag ggtattgaca
    36061 caaaatatta ttcttttttt tacagtcatt ctgttacaat tgtttgtttt taaccatatc
    36121 cagcttaatg ggcttattat tccttatttt tatgttcttt tattattgtt gctccccttt
    36181 gatatctcaa aatcgatgtt gttatttgtt ggatttgttt tgggtattat tatagacatt
    36241 ttttcattta ctccgggttt acatgctgca gcctgcacct ttgctgccgc agtaaggcct
    36301 actattttag agtggtttgc ccctcgtgat ggatatgagt ctggtacatt gcccagaatt
    36361 agttattttg gtttggcttg gtttataaaa tatagctttg tgcttattat ggcccaccat
    36421 atttttttgt tttttttaga agtgtccacc tttcatggtt tcttttcaac gcttttcagg
    36481 gcaatagcta gttctatatt cacgttgacg ttggttaccg taagtcaatt tttcattttc
    36541 cgcaaataag caggatatga accaatactc tacccggcag tatattatcg caggcatttt
    36601 cctttccgtc ttcctcattt ttcttgtccg acttttttat attcaaataa tagacagtac
    36661 ttacaagtat tctgctgaaa ataattcaca gaggtacgta accatctatc ctgcacgtgg
    36721 gcttatttat gatcggcacc acaaactatt ggttagcaac aaggcagcgt atgatttgat
    36781 ggtaaatccc caggaattgc gtgagtttga tactgccagt ttttgtaaga tacttaacat
    36841 caataaagaa tatgttgaca aaacattaaa aaaaattaag gactattcga agtataaatc
    36901 ttcgcccttt ctttttcagg tttcagacac aacatacgct gagttgcagg aaaagcttta
    36961 cttgtttccg ggcttttatg ttcaggcgcg tactttgcgg caatatcaat atcctatagc
    37021 agcacatttg tttggatatg taggggaggt tgattcatct atcattaaaa aaaacaatta
    37081 ttatcagatg ggtgattata ttgggatgag cgggcttgaa aaatcgtatg aagaatattt
    37141 acgcggggtt aaaggagtta acattttact tgtagatgta cacaaccggg ttaaaggttc
    37201 tcttcaaaat gggaagcttg acaggcctgc tatacttggg aaaaacctgg tcagctctat
    37261 agatgccgac ttgcaaattt atggtgaaaa gctgattaag aattttaggg gaagcattgt
    37321 agctatagaa cctaaaaccg gagaaatatt ggctattgtt tctaatccta cttataatcc
    37381 cagcttactt gtaggacgcg agcgtaccaa aaactttata gacttacgaa atgacaaatc
    37441 taaccctttg ttttacaggg ctattatgtc aaaaaaccct ccaggctcta catttaagct
    37501 gctcaatgca atgatagcat tgcaaactgg agttgcagtt ccttccacta caataggttg
    37561 ttatcatgga tatactgctg gccctgtaca tgtaaactgt cacgtacacc ccagcccttt
    37621 agatatgccc cattcaattc aacattcatg taatgcttat tatagtgtat tatttcggaa
    37681 gattatagac gagcagaagc tgccgcgtaa ccaagcttac gatgtttggc gaaaacttat
    37741 tatgtctttc gggttagggc aaaaaataca taccgacatt gccaatgagt taggaggaaa
    37801 tatcccatcc agtaattatt acgacaagat ttaccgcaaa tcttggaatt ctcttacagt
    37861 tgtatcatta ggcataggtc agggcgaaat aggtttgacg cctttacaat tggccaatgt
    37921 agcatgtgcc attgccaatc gggggtatta ttacgtgcct catgtggtaa aacagattga
    37981 aggtatagat acaattgatc agcgattttt gaagaagaat aaaacgttga taaatcctga
    38041 gtattttgat actgttatcg aggggatgta catggctgtt aacgggcctg ggggagggac
    38101 tgcaacaggt gctgcggtca agggcttaga tatatgtgga aagactggta cagctcaaaa
    38161 tcctcacggt gaagaccatt caatatttat agcatttgct cctaaggaca atcccaagat
    38221 tgccatctcg gtatatattg aaaatggagg ttttggagct acaatagctg ttcctgttgc
    38281 cagtcttatg atcgaaaaat acctgacaga ttcaatttca aggccttatt atgagcagta
    38341 tttagaaaca aagagaatta attacccggt aaaatgaaaa gggcatttaa cgtttggaat
    38401 aacctcgact ggtttacagt aatgatatac ttagcactgg ttattattgg ttggataaac
    38461 atatatgcag ctgtttattc ggatgagcac caacatatct ttgactttca gatgcgttat
    38521 gggaagcaga tgatatggat tatggcagct atatttttgg cgatagtagc tttagtaatc
    38581 gatattcgga tatattcgtt ttttgcctat cctgcttatc ttgcaacaat attattactt
    38641 attgctgtaa tatttttagg aaaagaagta aatggggccc gtgcctggtt tgaaattgga
    38701 gggctgcgga ttcaacctac cgaatttgcc aaaactgcta cctgtctggc attggcaaaa
    38761 tatttaagtt cttttgagat aaggattgat aaggtaaaaa caatgatgac tatattgcta
    38821 atcattgcca ttccatcaac ttttataata ttacaacctg atgtaggatc attactggta
    38881 tttgtgtcat ttgcttttgt gttgtaccgc gaaggcatgc ccggaggttt tttgtttttt
    38941 ggttttttac ttgcattttt gttcgttttt gctttaatag cacaaccgtt gactgtatta
    39001 atagtaatac tttttattgc agtagtagcc tttttcttat taaataaagg gacaaaagag
    39061 actcttattt tcatattcac gttagttggc ttatattttt taatatttat catagccaaa
    39121 ctatttcata taaaattatc ttattacaag acattattaa tagcttcgat tattaccggt
    39181 ataagtggac tggtgtatca attttttcga aaggtgccgt atgctttttg ggttgctgtt
    39241 atgatgttta tagctatagg gtttacctat tcgatagact ttgtatttaa caatattttg
    39301 gagtcgcatc agaagcaaag ggtcaatatt ttattaggca aagaatctga ccctcgcggg
    39361 gccgaataca acgttaacca gagtaaaatt gctataggat cgggaggttt tttcggaaaa
    39421 ggttttttac aaggcacgca aacaaaatat aattttgttc ccgaacaaag tacagacttt
    39481 atattttgta cagttgggga agaatggggg tttttaggct cgtttattgt tttggcgctt
    39541 tttggcttgc tccttgttcg tataatctat cttagtgagc gacagcgttc ggcttttagc
    39601 cgaatatacg ggtacggggt agcttctatt ttgttttttc atgtttttat caatgttgga
    39661 atgaccatag gtttattgcc ggtaataggc attccgcttc cattttttag ttatggggga
    39721 tcatcgctgt gggcttttac tttgttactt ttcatttttt tgaggctcga tgccgggagg
    39781 ttggagctct ttcattagtt ctgtgaaatc aataacagca agtctttcat aatgattcta
    39841 aataagtaaa agtttagaaa aaattatcct taatatattc ttttgtataa ttttgtcgcc
    39901 tttaaatttc agaagtaaaa agaaactatt atgaatcaac ttgtattagt cttatttatt
    39961 cttcctttgc tcgtgagccc attattattg gtattgccac gtaaagcgag gacatttgcc
    40021 acagtaacga ctgtaagtat tttatctttg atttcagttt acctgtattt tagggcagat
    40081 gaaaccatca gcatcatgct tccgggaata gtaaacacta ttatagcagg ggcagatgtg
    40141 atattgcttt tatattttgg ctatatagcc attcaacgca aaagcttgct tgtgggtatc
    40201 atgagtgtcc tgcagcttgg cggcgttttg tttttattga caaagatgcc tcaccacgaa
    40261 gcaaaccaga tattagttga caagttaagt ttatttatgt tcctgctcat caatattgta
    40321 agtggtgtta ttgcagcatt tgctattgag tatattgaag aggagaattg cagcgaatat
    40381 cgcaagcgct tttttctttc tgttatcatg tggtttattg gggtaatgaa cctgatagta
    40441 tcggccgata acctcgaata tttcttcttg ttctttgagc tgacaacatt ggcatcttac
    40501 ttgcttatca gcttcagaaa agacgaagag tctgtacaca atgcacttac cgcactttgg
    40561 atgaaccaaa ttggcggtat tgctatttta ggggctatat ttatggctgt atttacgccg
    40621 ggttatggtg aaattacttt ttcaagccta attgccaaag ctcctactgt tggtgtatta
    40681 ctcccttttg ctttattggc agtggctgca ttgattaaag gagctcaaat gcctttcagc
    40741 aaatggttgc ttggagcaat ggttgctccg acccctgttt ctgcattact tcactcgtca
    40801 acaatggtga agattgcgcc atttgtcatc ttacgtattt caccggctat tcacaatact
    40861 atgctagcgc aggtaattat aggcctaacg gcttttgtat tcctggtagc agctattagt
    40921 gcactggcac aagatacctt taaaaggata cttgcatttt caaccatttc tcttttaggc
    40981 cttatgtgct taatggctgc aataggttct gaagtagcag tagtggcagc acttttactt
    41041 atcatcttcc atggtttgtc aaaatgtatg ctcttcctca acgctggtat tattgaaagg
    41101 atattccact ggaaaaaagc ttcagatatg gacaaacttg gcgaagtagg cccattcact
    41161 tcattgatag tagcagtagg ttttatgtca ttgttactgc ctccatttgg cgcatttgtt
    41221 ggcaaatggt ttagtttgga aactataggc gctggtcttg gtaactcaca ggttgccggg
    41281 gctcttgtta ttagtgcaat tgccattgga ggtgctgtaa tgtcattgct ttactttaaa
    41341 gtaatggggt taattattgc ccgctctggt gaaaaagata aagtacagtt tgaaagcatg
    41401 ccaattcttt tctcatcaag tatgtacatt ttacttgctt taatatttgc tggtattatc
    41461 agcttaccat ttatgatggg agggttcttt gccccggtag catctgatgt tactggatta
    41521 gcagttaacg ttcaatggag caagctgacg atgatgatag gagaagctca attgccaatc
    41581 atcccattgt tcctggcttt cttgtttttg cctttgacca tgataatagc catgtttgtc
    41641 cgttttaaag gagctgacag ggctaaagaa tatatgtgcg gagaaaaggt cgaatacagg
    41701 ttgtcttcat tctacttcaa tactgaaaaa gttactccat atttcattgc aataggtatt
    41761 atattcttca ttgggattgt taccgttgct attatttaat tggttaatgt aaaattttat
    41821 agatatgaac agtatcatta tttgtaccat cattgttata ctggctccat ttattggagg
    41881 cttggtatat ggtatcgagc gtaaggtaaa agcacgcatg caacatcgtg ttgggcctcc
    41941 aattctccag ccattttatg attttatgaa acttgtcgat aaaagggcta tgatagttca
    42001 ttcagctcat gcttttttgg gcattatgca ttttatagca ttatggtttg cagtagctgt
    42061 tttaatcttt ggtggcgact ttattttggt tatttacctg cacctgatgg ccacggcatt
    42121 gcttatcatt gccggttata gcaccaggtc agtatttagc caccttggtt caaaccgttt
    42181 agccataagt gctttggcat acgagccagt actcattctt attgctattt ctgtttacct
    42241 aaccactggt agctttgaga tttcgtcaat atttagtatg tcaacaccgc ttattgcaaa
    42301 aatgccttta gcatttgttg cattgttgtt aatattgccc attaagctca aaaaatcgcc
    42361 ttttgacgta gcagaagctc accaggaaat tacaggtggc gctgagattg agtattcggg
    42421 tattttcttc gaattcttat atacagccaa atggcttgac tatgtatttt gctatgtatt
    42481 agtttacctt tttgcagcaa acaacgtttg gcttggatta ggtttagtag cagctacatt
    42541 tttatatatt aatgcccttg acaactctac tgcccgcgtg gactacaaac agatgctgaa
    42601 attcagtatg tatgtagcat tgccaatagc atttatcaac cttttactca ccatcttaaa
    42661 gtaaagatat gaaactcagc ggttatcgta aaaaatcacc atggatatta cattacaatg
    42721 ccggaagttg caatggttgt gatatcgaaa ttcttgcatg tctttgtcct aaatatgacg
    42781 tagaacgttt tgggcttatc aataccggta atcctaaaca atcagatatt ctgctcatta
    42841 cagggcctgt aaccaaacgt tcacgtgaac gtctggttga gatttatgcc caaatgccag
    42901 agccaaaagt agtcattgct tgtggttcat gtgccagtac aggtggggta tttcgtccga
    42961 tgtacaacat tgaaggtgga gtagatcagg ttatccctgt agatgtttat gttcctggtt
    43021 gtgcttgtcg tcctgagcaa attatagatg gcgtagttac tgctcttgga atacttgaac
    43081 aaaaaaccaa agaaattgaa aagagccaca ttcttcatgt agcaccatta gaagaagacg
    43141 gacaggtaaa ccgccggcac atggccggga ccattaaaaa gtgatttgtt ttgaagtttg
    43201 taaatttttg aaatacaaat gtctatgaaa aagatagaaa caacgctcga aactttgaag
    43261 gccgatattg gggcttttta taaggtggat gagcaccatt ttatagtcat gaacgttgta
    43321 gatttaggtg gtaaccaact cgatatacag tggttcttta gcgactatgc acaaccttgc
    43381 gaggtaacaa tgtttacagc taaagctgat gccggagttc aaattccgtc tatcaattca
    43441 atagtagcct ctgcatgggt tgctgaagct gagttagttg accttatggg ggttaatatt
    43501 gaaaatgccc aaaaaggttt tgttcttgaa gatgattttg aaggttcacc attaagaaaa
    43561 gtaaagtaaa ctatggcaac acaatatgat attccggttg gggcgcaaca tatatcgttg
    43621 ctcgaaccat tacattttaa gctcactaca gaaaacgaaa aaattgttga cgttcaggcc
    43681 aacgtgggtt atgttcaccg gggcattgag ctggcatgta cctctaagtt taaatttaga
    43741 caagtaagtt ttgttgttgg ccgtgtttgc gggctttgct ctatttcgca caccactgca
    43801 tactcacagg tagctgaaaa actgttaaac cttgagcttt caaaaagggc taaatacctt
    43861 cgtattttag tactagagct tgatcgtatc cactcgcact tgttgtgctt atcgcacgta
    43921 gctgaaaatt cgggttttga ggcgcttttc atgaaaacta tgaaagaccg cgaaatttcg
    43981 atggagttgc tcgaagctgt ttgcgggaac cgtgtacaat atgactattc ggtagtcgga
    44041 ggtgttgccc gcgatttgcg cccagaggtt gcacaaatgg tattggcacg tttgaacgat
    44101 ttcaagccaa aactcgatga tctgagagaa atttttgaaa acaactattc gctttcttta
    44161 aaaaatagag gtattggagt attgagcagt gaaaacgtga agctgcttaa tgctgtaggt
    44221 ccattggcac gtgctgcagg tgttcctatt gatgtgcgta acgaaactga agaccttctc
    44281 ccatggaaag aagttggttt taatatggtg cttggcgatg caggcgacgt tcatgcccgt
    44341 aacatggttc gtatccatga gttgtacaat tcaattcaga tgattgaaaa tgttattaac
    44401 ggtttgcctg aaggagatat cattacagaa actaaagcta tgccagaagg tgaagctgtt
    44461 gtacgtctgg aagctcctcg tggggaactt ttccactatg ctaaagggaa caagacccag
    44521 attttggaac gtttgaaaat caaagctcca acattctcaa atatcccggc atttattgag
    44581 acattcaaag gtaacgaata tgcaagtgcc ccggctatca ttgcttcatt cgacccttgt
    44641 ttgagttgta ctgcacgata attataaact gttaacagaa gtaaccatga aatcattttt
    44701 cgaagcgtta acaaaccttt tcaggaagcc agctacggta aaatatccat ttgaaaaaac
    44761 atatatcccg gaagactaca gggggatgat agttcataac cctgaacttt gtatttggtg
    44821 ccgcaaatgc gagatggtat gtcctccagg cgctattgtg ttttcacaag atatggatgg
    44881 aaaacaaaca taccactaca atcgtgctgt atgtatttat tgtggcgaat gtgtccgtca
    44941 atgcccaaaa gatggggcct tgatgcaatc ggctactcct ggtacccctg ctgttaaaaa
    45001 tgaaaatatc aacaacagtt ggaaggtcac ttatgatgaa gcattaaaga gccgtgctgc
    45061 atacatggct gctaaaaaag ccgctgctgc ttcaaaatct gctgaagggg ctgctgaata
    45121 attagctttt tgttttagac ctaaataata aatcccggga ggtttgtctt tcgggatttt
    45181 tatttacatt tttcttccag ctcaatatat ttgctgacaa tgctgagaaa tagctcaggt
    45241 aagacaggtt tgttgaggaa atattcgatg ccaagatcat atatttcttc acatcgttca
    45301 gtgtaggcgc taataaaaac aatagggact ttaaatcgtt cgttaattaa gaattttgtc
    45361 aactcaatgc catcagtgtc tggaagcata aggtccataa gtatcaggtc aaaccttacc
    45421 ggtttttttg catgatatta taaaattctt ttccatctgc agctcttacc atttctacat
    45481 tatattctga aaggatttct tccagcaaat aataatttgt atcgttgtct tctatggcaa
    45541 agactctttt cataaatgcc tgtctgctat tttttttgtt gacttaatgg tactaagtta
    45601 atgaaaaaca aaaaaatggg caggtgtgtg tgttattttt caattgatgc tcgtcaaaaa
    45661 atttgactat tgcgattatt ctttattctc gaaataattt tcaataatat cgcgaatgga
    45721 gccatgcatt aaaggtttaa gagggattat ttcgttgtaa ggtaaccaca aagcacgttc
    45781 tatatcttca gatgtttggg gttgaaggtt tttgtttgac ttacactgca taaggtacca
    45841 gtgggttttt ttcaaaacca attcttcttt taaaaaataa agatgataag tatgttgaaa
    45901 aaacctgttg atagaaagct ttttagctcc ggtttcttct tctacctctc taatagctgc
    45961 tttttcgggg gtttcgcctt tgtctatttt acctttggcc atatcccatt tgccacgtct
    46021 gaaaatcatc aaaaaatttt tatctgcatt ttcgaccaag cctcctgcag cttcaataat
    46081 cctgaacttc gaagcaaaaa gtttaaaagt accctctaca tcttctgttg taaaacattt
    46141 tgtttcagag gtattattgt tttgagctgt agcaagccaa tcaataaatt gtttttcatt
    46201 ttcaaagggg attgcttgca gtttaatctt gtcagcctcg tttttattag ctatgactaa
    46261 aaagctattg ttaaaaaaaa ctttatacat ttgtggcctg aattataagt gtaaaaacgt
    46321 tgtaaagata tggaatatag caaaaaaatt gctcaatttt tgattgaaag ccagtcggta
    46381 aaacttcgtc ctgaagaacc ttttacttgg gcatcgggct ggaaatcgcc tatttattgc
    46441 gataatagaa aaacattggc attcccggct atccgcgaat atattaaaaa tgctttcgtt
    46501 gatatgatca ataaaaagtt cccggaacct gatgttattg caggtgttgc aacaggggct
    46561 attgctcagg gtgcgctggt ggctgatttg ctgaaaaaac cattttgtta tgtccgttct
    46621 gcccctaaag atcacggaat ggccaatctt attgaaggaa accttgagcc aggtcagaag
    46681 gttgttgtaa ttgaagatct tatatctacc ggaggaagta gtattaaagc tgttgaggca
    46741 ttgagaaatg ccaaagccga tgttcttggc atggtggcta tatttactta tgggttcgaa
    46801 tcggcaagtc agaattttaa aaaagcaggt atcaatgttt atacattgtc ggattatgac
    46861 actcttttag aacaggctat ttcatgggga tatatcaatc cgagccattt gaacctgctc
    46921 aaagaatggc gtgttgcacc ggataaatgg gggaatgcat aatatggagg taaaagtaga
    46981 gagccgtgtt ggtaaaatgc ctgtcaatac tgaggctttg tataggttgt tttcagactt
    47041 cagaaacatg acacagttta tccctaaaga taagttgcgt aattttcagg caactgaaga
    47101 tacctgctcg tttgaggcag atatggtggg gttagtaggg ctgaaaattg ttgaaagaaa
    47161 gcccaattcg cttgtaaaga ttatttcttc ggagatctcg aagtatagtt ttactttttg
    47221 ggtacaattg aaggaggtgg caactatgga tacccgcatt aagctgacgt tggcttttga
    47281 atctaacccg tttatggctg caatgttcaa agggcaggta gaaaaagctt tgaattctat
    47341 gattgaccga attgagcaac aaaacttcag ctataactga tgtttgtaca agccaaaaaa
    47401 aaattaggcc agcattttct gaatgatcag aatattgctt tgaagattgt tgaggcaata
    47461 gaaggtcaac ctggccttgt cattgaaata ggcccgggaa tgggcgtttt gacgcaattt
    47521 cttgtaaaaa aaattgaaaa ttttattgcc attgagatag accgcgaatc ggttgaatat
    47581 cttacaaata acaatattat cagtgaaagt aagctctttc gtcaggattt tctgagggct
    47641 gacttgcaaa accttttgaa aaacaataca tgtattattg gcaatctgcc atataatatt
    47701 tccagccaga tatttttcaa agtgttggaa aataaacagc agataaccca agtcgtgtgc
    47761 atggtacaaa aagaggtggc agagcgtatt gcagaacctc ccggttctaa aacctacggc
    47821 attcttagtg tttttttaca ggctttttat gatattgaat atttgtttac cgtacacgaa
    47881 catgtgttta cacctccgcc taaagtaaaa agcgctgtga tacggcttaa acgaaactgg
    47941 agacttgaat tgccttgtga cgaaaagatg ttttttactg tggtaaaaac ggcattcaac
    48001 caacgtcgca aaacaatgcg aaattcgctt cgtccacttt atcctcctga ggcgcttgcg
    48061 gacccggtat ttgacaaacg ccctgaacag ttatgtgttg aggagtttat agagttaaca
    48121 aagatgctgg agccaaaaga ttaaataatc caatctttct ttttcttgta aattacccaa
    48181 tatccaatga ttccaataat gggcaagagc gtaatcatgg ccataaaggc atatttgaaa
    48241 ataggctttt tgtaagtttc gtgtaaaata atgaatgcgg tataaacaaa gcctaccgca
    48301 aaccctacta tgatgagccc tcctaaaata cttccaaaca tctttcttat tctgcgtcta
    48361 attcaaaagg taaacgggct tgaatgcctt gcatactatg tactttgtta gctatacgaa
    48421 tatactttgt atattctgca tattttccgg tgaacaattt catcataact tcaccagata
    48481 gctcgttgat aaacttttcc catgggtctt gttgtaaagg gaggtttaca accctatact
    48541 tttcaacttt agccattttt gctgcttttg caatggcatc atctatatag cctaaggtgt
    48601 caaccagtcc gattttgagg gcatctgtag cagcccaaac acggccttgt ccaatattgt
    48661 caacatcttc agctttcatt tttcggcctt cagcaacgtg attgataaag gtgttgtaaa
    48721 tacgttcaat ctggctaagc atcacttttt gttcgtattg gtccatagcc cttgtcccgg
    48781 atgggaagtc tgagtgtcct tcagtcttta ctacatcggt ggtaatgccc agtttttcat
    48841 ttaacaattt tttggcgttg aacatcactc caaaaacacc tattgaacct gtaatggttg
    48901 aaggttgcgc tatgatataa ctggcagggc aagcaatgta ataacctccg ctggcagcta
    48961 aatcgcccat agaagctact acaggttttt cagcaacagt aagggctact tcacgccaaa
    49021 tgatatccga agccagagcg ctgccaccag gagagttgat acggaacaca actgctttga
    49081 ttgacgtgtc tttgcgggct ttgcgcaatg ctgctgaaat cttttctgac gaaatatacc
    49141 catctgcggc atctcccatc actacatcgc caaaggcata gactacagca attttgtttt
    49201 tggcaacgcc ctcacccgta acagctttag gaacattttt gtatttagcc aaagtcacca
    49261 gtttgggttc cttgttgctt gaaccactta tttttaagag cttagcgaaa atctggtctt
    49321 tatataacag actgtcaact atgcctgttg caacacaact atcagcatct gatgtattga
    49381 gggtgtttgc tatttttttt actttttcag ggcttatatt tcttgatttt gcaatgtcat
    49441 ttactgtgtt gttccaaata gcatcgagca ttgcctgaag ttgctcgcgg ttttcggggc
    49501 tcatcttgtt gttcaaaaaa ggttctacgg cacttttaaa tttaccatga cgtataattt
    49561 gggcttcgat gcctaacttg tccaaagcgc ctttaaagaa catcacttca gcctgtgccc
    49621 ctttgaagat gatactacct tctgggttta gatatacttc gtcagctaca gatgcgagat
    49681 aataagctcc ttgtgacatg tattcgccat aagcaatgat cttctttcct gaagttttga
    49741 attggagaag ggcattgcgt atctctgaaa tggttccaat acttgcctga ataactgaaa
    49801 gctctaagta gatgccccga atgttggggt caacttttgc tttttcaatg ttttcaagaa
    49861 tttcgttaag cccgtcttgt ttagattgtc cgtagcctat gtcaatattg tcaagaatcc
    49921 ctttggagct tctttcaaca atttcatttt caagagtgag ctttaatatg ctgttgggtt
    49981 caatgctggt agctttttcc atgtctgaaa ctaaagcact cataatgcca ataaagacaa
    50041 aaaacataat gatgccggct ataaaaacgc ctaatattga ggccaataag gttttaaaga
    50101 agcttttcat aaagttttat ttttatttct gataagacaa caaaggtcat attttcccga
    50161 aacaaaatta taaataaaag gcttaaacat aattttcact aagttattta caatgctttc
    50221 agtggcttat tttttgtttt ttaaatttag ttttaaatat atataaccta ttattccggc
    50281 tgttaaagag gctaaaagga tagctatttt tgagcctgca acaagatgag ggtcgtcaaa
    50341 tgcaagcaat gtaatgaaga tagacatggt aaacccgata ccggctataa ttccggcacc
    50401 aataatatta gtccatttta agtcttttgg aagggcactc catcccaacg caacacttgc
    50461 aaaagcaaat aaaaatattc caataggttt gccaaaaatg agccctgtca ttataccaat
    50521 gctgttatcg tgggtaagcg atgcaaacca gttgggctca ataacaatac aggtattggc
    50581 catggcaaat ataggtaaaa tgataaatgg gactggcttt tgcaacgcat gttcaagaat
    50641 agaagaaggc gattttttgc tgccatcgac aaatggtatg gcaaaagcaa gcaatactcc
    50701 ggtgatagtg gcatgaactc ctgaatgtag cataaaatac cacataattg tccctcctat
    50761 gaggtaaata aagagttttt taaatttaaa ataattaaat aaaagcaata tcccaaatat
    50821 accaagggct attaataagt tgagaacaga aaggtccgaa gtataaaaca aggcgataac
    50881 tataatggcg cccaagtcat caatgatggc caatgccgtt aaaaagattt ttagagaagc
    50941 tgggactttg ttgccaagca gtgacaatat cccaatggag aaggctatat cagtagccat
    51001 ggggatgcct gtacctgatt gggttatttt tccaatattg aatgctgtat atagcagcgc
    51061 ggggacaatc atccctccta aagccccaaa tactggcaga gaggcatttt tgatgttaga
    51121 tagttctcct ttgtatattt ctcttttgat ttctaaacct attaaaagaa aaaacacggt
    51181 cattaggcca tcgttgatcc aatgttctaa gttgtggttg gcaaccggaa acccccaaat
    51241 acttttgtag gcatcatgcc atgaagtatt ggccaatatt aatgatataa ttgtgactgc
    51301 aacaagaata ataccccctg ttttctcgtt ttccaaaaat tctttgaaaa tctttgaaaa
    51361 ccttgtttct gaaattcttt tctttatcat ggtgaaaatt tgtttatcag tttgtaaaat
    51421 taggaaaaca atttttttag gttgctaata ttcaatattt attgtgcatt aatgattcaa
    51481 tggcatcagc gtcaataggg atgtcaatac ttaagttgat accaccatcc tgagttatca
    51541 ggatatcatt ttcaagacga atgccaatac cttcttctgc aatatatatg ccaggttcgc
    51601 aggtaataac catattttct tccagcgtat catgtttgtt gttggggtca tgtacatcaa
    51661 ttcctaatga gtgggctact ccgtgcatca gatattttct gtacaaaagt ttttcgggta
    51721 gttgcttttt gagctctgct ggtgtaatta gtttgaggtc aacaagctct ttttcaataa
    51781 aaggtattgt ttgttggttt agtgcttcaa tagaaatacc tggctttatc atagatatga
    51841 tgttgttgtg aagcctgtac acggcttcat acacttggcg ttgcctgctt gtaaatcggc
    51901 cgtttacagg gacagttcgc gtaatgtctg cattgtaata gttgtgttca gcgccaatat
    51961 ccatcaaaat aaggtcacca tcgttgcaaa tgcagtcatt attgatgtag tgcaagatac
    52021 atgcatttgc cccacttgca ataattggca aaaatccatg cccatttgct ccatttcgaa
    52081 taaattcggc tgttatttca gcttcaatct cgtattccct tttccccggg ctgattactt
    52141 tcagagaact gagaaaacct ttgcgggtaa tgtcaatggc ttttttgatt tgttgtattt
    52201 ccgcttcttc cttgattctt cttaagctgt gaatgatagg gctcaggcgt tcaaagtgat
    52261 gcaagggatg aacctcttgt atataatgtg ccagcttgcg ctgattaacc ggaatgtcgt
    52321 atgcggtttt tgcaatatct gtaattggca ggtaaaatgt ttttattttt tcggcgaagc
    52381 ttgaaacgat tgcttcaaat tcattagcca acattatttt ctttattcct gataagaggg
    52441 tagctttttc ggaagttaaa atattgccgt tccacgtttt atcgtgttct gtaatttcgg
    52501 ggataaacaa aacttcccga aattcagctt tgtcatggtc gggcgaaagc attagaatag
    52561 cattgggatg gtttatacct gttaagtaat aaaaatcact gttttgtctg aatggataat
    52621 gttggtcagc agtagaaatt atattacttg cggcaggtat tatggctaaa gaatgtggtg
    52681 gcagtatttt caacagtttt tgtctgtttt tttcaaaaaa gtaactgttt attggtgttg
    52741 tacgcatagg atataaattt tttgtataaa agtcaaaagg caattacatt ttttgctttt
    52801 ctcctttcgc ctcatttttt ctctcttagc ttttttatca ggctatttaa catctttttc
    52861 tataaatagt tttagcatat tccctcgaga tgaaatttac ttttatagct atttcgaacc
    52921 gggtttgtaa tgcaaacaaa gaacctccac tttatttcca gaaagtgtat gttgatatcc
    52981 atatctttct gcaatgcctg aagggataga cattgcactt ctttgagatg ttagcgaaaa
    53041 aatttcttct ttcagtaatt gcttagtaag tgcatagatt tgttttacca attcaatagc
    53101 tttctgccat acaatcaaat ctctataaga attcataatc aaaatataat tacaatcatt
    53161 ccctttttat gccttttcct ttctttcttt ctttctttct tgtatcttat ctatactcaa
    53221 tctaaatagt gaaaattttt gccagatcac attcaagcat ttaaatttgc agctcattaa
    53281 aacgagagaa gaaaatcttt tcaagtgcaa tttaaaaaaa ctttattgaa ttgatatgag
    53341 taatctggta aattcttcta ttggcaaaaa gctgatgatg gggcttgcag ggctatttct
    53401 gtgtgctttt cttttggtcc accttgggat caacttattg attttgttaa atgacggagg
    53461 tgaagcttat cgaatgggag ttgaatttat gactaccaat attcttataa aaattatgga
    53521 gatatttttg tttggaggtt ttttgcttca tattgtttat gctatctatg tacaggtcca
    53581 aaattggttt gcgcgcccga tacgttatgc aaagactaac cattcacaga cctctttctt
    53641 ttctaagtat atgattcata caggtgttat tataggcatc ttcctggttt tgcactttat
    53701 caacttttat tttgttaagc tgggtattgt tgctgtacct gaaggagcag aggggaagca
    53761 tgacttctat aacatggtgc ttaacttgtt caaaaaccct gtttattcat ggatatatgt
    53821 agttttcatg attgggcttg catttcacct caatcatgca ttccagagtg catttcagac
    53881 tcttggctgg aaccacaaaa aatacacccc gattatcaat tttgttggaa ctgcatattc
    53941 tattgttgta cctgcagggt ttgctattat tcctatatat gtgttgtata taatgtaagc
    54001 tcctctttaa tcacccccaa ggggaggatg ctaaagcaaa tgattaagaa agtatgaaat
    54061 taaaatataa tataatttag agttgacttt cgcccctctt ttggaggggt tgggggaggc
    54121 ttcttaaata ttttttgtat gagtttaaat tcaaaaatac catcagggcc actcgaacag
    54181 aagtggagct attacaagag ccatcaaaac ctggtgaacc ctgcaaacaa gcgtaagctt
    54241 gatgtaattg ttgttggaac tggccttgca ggggcatctg ctgctgcttc acttggtgag
    54301 cttggtttta atgttaaagt tttttgttac caggatagtc cgcgtcgtgc ccatagtatt
    54361 gctgcgcaag ggggtattaa tgctgcaaaa aattacccta acgatggcga tagcgtatat
    54421 cgattgtttt acgatacaat aaaaggtgga gattaccgtg cacgtgaggc aaacgtttat
    54481 cgtctggcag aagtaagcgg gaacattatt gaccagtgtg ttgcccaagg ggtccctttt
    54541 gctcgcgagt atggcgggtt gcttgacaac cgttcattcg gaggggcaca ggtaagccgt
    54601 acattttatg ctcgtggcca gaccgggcaa cagttgttac ttggtgctta cagcgctttg
    54661 agccgccaga ttgaaaaagg tacggtgaaa atgtacaacc gtaccgaaat gcttgatgtt
    54721 gttcttgttg atggcaaagc tcgtggtatt atcacccgta accttgtaac cggggcgatt
    54781 gaacgtcatt ttggccatgc tgtggtactt gcaacaggtg gatatggcaa cgtctttttt
    54841 ctttcgacca atgcaatggg ctcaaacgga agtgcagcta tgcaagctta tcgcagaggc
    54901 gcatactttg ctaatccttg ttttgcacag atccacccaa catgtatccc tgtacatggc
    54961 gattatcaga gtaaacttac cttgatgtca gagtcattgc gtaacgatgg ccgtatttgg
    55021 gtgccaaagg ataaaacggt atctgaaaaa cttcgcaaag gcttgatgaa gccaacggat
    55081 gtgaaagaag aagaccgtga ttattacctc gaacgccgtt accctgcttt tggaaacctc
    55141 gttcctcgcg acgtagcctc gcgtgctgca aaagaacgtt gcgatgcagg ttatggggtg
    55201 ggcgctaccg gattggctgt atatcttgac ttttcaactg ctattcaacg tttgggcaaa
    55261 catgttattg aggaacgtta tggaaatctt tttcagatgt acgaaaagat tgtagatgag
    55321 aacccatacg agaccccaat gatgatttat cctgccattc actatactat gggaggtatt
    55381 tgggttgatt acgagcttat gactactatc ccaggtttat atgccattgg tgaagccaac
    55441 tttagcgatc atggcgctaa tcgccttggt gcttcagctt tgatgcaagg tttggcagat
    55501 ggatatttta ttttgcctta tactattcaa aactatttgg ccggagagat tcgtactccc
    55561 cgtatgagta ctgatgctgc tgagtttgtt aaaactgaag ctgaggtaaa agagcgtatt
    55621 aaaaagatta ttaacattaa gggcaagcat agcgttgatt catttcacaa acgtcttgga
    55681 catatcatgt gggaatatgt tgggatggga cgtacccgcg aaagccttac caaagctatt
    55741 gaactgattg ttgcccttcg tgctgaatac tggaaagagg tatatgtacc cggaacaaat
    55801 gatgagttta atcctgaatt ggaaaaagct ttgcgtgttg ccgatttctt agagctgggc
    55861 gagatgatgg cccgtgacgc cctcaatcgt gaagaaagct gtggcgggca ttttcgtgaa
    55921 gaatatcaaa ccgaagatgg agaagcatta cgtaatgatg ataagtttat gtatgcctca
    55981 gcttgggagt acaaaggtga tgcggctcct gaaatgcata aggagccttt aaattatgag
    56041 ttcattgaag taaaacaaag aaattataaa tagagacaag agccaagaat caagaacaaa
    56101 gagtatagac tcttgattca atgatgactt tggtgtaaga gctttataaa tttaaaaatt
    56161 aaattttctt gattcttggc tcttggttct tgattctaaa aagataaaat atggaatcaa
    56221 aaggtttaaa cctcacccta aagatttggc gtcaggagaa cgctaaggca aaaggaaaat
    56281 ttgtaacata cgaagtaaaa aatatttcgc ctgactgctc atttcttgaa atgcttgacc
    56341 agttgaacga aacactggtc gaaaaatttg agatgcccgt ttgctttgac cacgattgtc
    56401 gcgagggtat ttgtggtatg tgcagtttgt atatcaatgg caggcctcat ggcaatgata
    56461 gtggagttac tacctgtcag ttgcacatgc gtaagttcaa agatggcgat actatttata
    56521 tcgaaccctg gagagctaag gcatttcctg taattcgcga cttggttgtt gatcgttctg
    56581 cttttgacaa aattattcag gctggtgggt atgtttcagt caataccgga ggggtgcctg
    56641 atgctaatgc cattgctatt cgtaaagaaa atgcagactt ggcaatggat gctgctcaat
    56701 gtattggttg tggcgcttgt gtggctgctt gtaaaaatgc ttcggctatg ttatttgtgt
    56761 ctgcaaaagt ttcgcacctg gctttgttgc cccagggcaa gattgaggct aaagaacgtg
    56821 tagaagctat ggttagccgc atggacgagc ttggtttcgg gaactgttca aacactggtg
    56881 cttgtgaggc agagtgccct aaagaaatta agattaccaa tattgcccgt ttgaatagag
    56941 aatatatctg ttcgaaactg ggttagttta aataaacaca gaggcacaaa aacacggagg
    57001 aacactgtgt ttttgtgcct tttttaattt attattaaac tttattttta atctatggtt
    57061 tatcaacaca agtacataaa tttattggaa tagattatat ctctgtgttt tagtgtcttt
    57121 gtgtttaaaa gtattgatta tgaaagaaaa aattgaagaa gcccttcgca atgttaacca
    57181 ccctgctaaa gggacagaca ttgtaagtgc tcaggtgctt caaaatattg agattgttgg
    57241 caataaaata attctgacca tgaattatgg taaagcgaat gacccatttg ccaattcaat
    57301 taagaaatct gtcgttaaga ttttagaacc tgttgtagga agccaatata ttgttgaatt
    57361 tgctgaagtt aacaacagaa agccattgtt acctgagtat tatacattga aagaggtaaa
    57421 aaatatcatt gctgtgtctt ccggcaaagg tggagttgga aagtcaacag tagcaagtaa
    57481 cctggctatt gccttgtcgc acacaggggc aaaagtggct ttgcttgatg cagatgttta
    57541 cggcccttct gctcccaaga tgtttggtga agagaatttc aggcctgtca tgattgagaa
    57601 agatggaaaa gaatatattg agtctttgca gaaatataat attggggtac aatctattgg
    57661 gttctttgta agccccggtg aggcaactat ttggcgtggc ccgatggctt ccaatgcttt
    57721 aaaacaggtc attactcaaa cctggtgggg agagcttgat ttccttgttt tggatatgcc
    57781 tccgggcaca ggtgatattc atttgactat tgtccaggag ctccctgtta caggggttat
    57841 tgtagtcagt actccccaaa atgttgcttt agcagatgct ataaaaggta tcagcatgtt
    57901 tcgtagcgat aaaataaacg ttccggttct tgggttggtt gaaaacatgg catggtttac
    57961 gcctgctgag ttacctgata ataaatacta tatttttggt aaggatggat gcaaaaaact
    58021 tgctgaatcc ctcgaattgc ctttgcttgg ccagatacca attgttcaaa gtatttgtga
    58081 agacggcgat aacggtactc caagtgcaat gaaccctttt acccctcagg gaaaagcttt
    58141 tgccgaattg gcagaagctg tgatagctca agttgagatt cgtaacaaaa ctctacctcc
    58201 gactaaaaag gttgaagtga cgcagaaata aatcctgcta tcgattcgca agaattttta
    58261 tgataggttt atcagataac attttccttc attacttttg atatgtcccg aagacttgta
    58321 atacctgata ttcatgggtg ctcaaaaacg tttgaagcct tacttgagaa aatagtattt
    58381 acacgtgagg atcatctttt tcttttggga gattacattg atcggggacc tgatagtagc
    58441 ggagtagttg attttattat caggttaaaa actgaaggtt ataaaattgt tgccttgagg
    58501 ggcaatcatg aagatgattt gctgaaagca tctcaatctt ttgataaaga tagttttgtt
    58561 ttttatgtaa accgttttaa aagtggagat ctattgactg ctgagggtca aataaaagaa
    58621 gagtacaaaa cttttttcaa tagtttgcct ttttatttcg agctgcctga ttttatcttg
    58681 gtacatgcag gagtcaattt taaaattcaa aacccttttg aagataagga tgctttaata
    58741 tttttgcgca atacagatta taatgcccac aaagctaaga accgcagggt tatttatgga
    58801 catcagccta cttatattaa tgatattatg gctgctattg atgaacaacg ttttcttatt
    58861 cctttagaca atggatgtgt gtataattca ttacacaagg tttatgatta caggtattta
    58921 ggccacttgt tatgtctcga ccttgatagc tgggagcttt cgatggaaag aaaccgggat
    58981 acttagttgt aaaagttcca ggtcagtcca tattgcataa caggtatcat taaagggtag
    59041 tgagctgcta cgaatgtatt tttcttataa aatccggcgt tgatgtgtga gtatttaaag
    59101 aaaaagctca caaccttcac tttgaaagtg aggtgtacat caacaacagg ataatcgcct
    59161 gttttggata ctttttcatt agcagaagtg agtgcaaaaa ctcccaatga aggtatataa
    59221 gtttctggtg tgtagctgct ttcatatttt acatcaaatc ccaggcgtgc atatagcttt
    59281 cctcctgttg agaacctgaa agtatgctgg atattggtag agttgtaata aaccatttgt
    59341 ggtacctgga agtaactgtt atcaaactcc tgaaaagtaa tagcattggt ggtttcaaat
    59401 ttccacaaac gtaaagtgtt tgaaaatgtt atacttgcaa catgcagcgt ttttgaagtt
    59461 tgtgtcacac cttcggcatc aaaatatacc atgttagaaa taatatggta atttccgcta
    59521 atagtagtat taagcttatc tgcagagatt gagccataga ttctttgctt atattcgcgt
    59581 ttgaaattat cgcctttgtt ccacataaaa tggtttgaaa agagcgaatt gtacataaac
    59641 gaaggcttat gtaattcaga aacaaagcct gcatcaaaag agaagtaact attagtgtct
    59701 ttgttcatcc acagtctggc atccaatcgt tgatcgccaa tattatagcc tgccaggtga
    59761 ctttcccata caagtttgcc tttcatccgc tttattgatt tctcaatgcc tgcagtaaac
    59821 caaaatttat agtagtcgct gaaagatttg tccgcgttcc cttttaatac tatggtgtcg
    59881 ttgctaagct gacctgtgta agttcgatag ccataaagcg gggcaccctt tgcattggca
    59941 gaatcaaagc cgggaatata aatattgttt ttttgaggga atggtggatt aaaatgtgtg
    60001 ccatttatta tcaatgtatc aggcacacaa cctctgaatg ccgaatagta tccaacgtgt
    60061 attgatccaa caaaatttgg cagatacttc caaagctcgg gcgatttaag tattactgcg
    60121 ttttcaattg tggtaagcga aagcgagtca ttagatattt tgctggcgta atagttgtgt
    60181 ttataaaacg aaacagtatc catttcagtt tctttataca actttttttg cgagtttaca
    60241 tcaagtttat aagtaatgga acctgattta attttgttga aaaaattagt actatcgtca
    60301 ttgatgcctt gtaaaaagtg caactggtga aaagtagaaa agcccatgcc ccgatatctt
    60361 gatttagcag tttcaaggtt aacagctacg cctttaaaat cgccgttttt gtaatttatc
    60421 aaggtgtcat tctttactcc gcctgattct tgcaaggtag tactattcca aaatagttga
    60481 aggtaggagt ctatactttc tcttttttta aaatagttga taatgaaatg gtggttactt
    60541 gcactgggaa tttcattggt agcagcgtat gaataaaaat tataatcaac gccaatcgaa
    60601 gaatttttac ccaatgactt ggcaaacctg accccggcat actcttcttt atcgctcaac
    60661 cctccggcta ttttgtatgt aacacttgaa aaagtcttat tcaaatggta aaaagacaat
    60721 tctggcgttt ccctgaaata catggctatt gatggataaa agttcgtatt catcgtcaaa
    60781 cggtctgata agtcgtttga aagagatgga tatccattat aagtcaggta cgagtatgat
    60841 ttctcgggtt tttgataatt ctttaaattg tgcaggtggt tgatagatgt gtctatcgca
    60901 acagtgtctg ccaataaatg atagtcgagt tttacctcag ccagataggt tgtatcgcgc
    60961 tttggtggtg ttttcttttg cgaaaaagcc tgcacaacaa ataacaggct aagaactgta
    61021 aataaaactc tcatgcaaat cttgattggc actttaattt taaaacgcca aagatagaat
    61081 aaaattacga atgactaatt acgaatgact aattacgaat gacgtatcaa tgtcgtttag
    61141 ttaattatta aaaaaagcca acaggcctga aagacgctgt cgggttagta tcacctctga
    61201 gcttacagcg ttcgcagaac ctgtccaggt cataactaaa caacattgaa ttactaaata
    61261 tgaatgatgt aattcgtaat ttttaattga aaattagtca tcagtcatta gtcatcagta
    61321 attggcttat ttgctttgca tggtttttgg tgtccacctt ttcaatgata tgggcaataa
    61381 cccctttctc atcaatgagg tacgtttttc ttaaaatccc ttcatatttc ctgccataca
    61441 tgcttttttc gccccaagcg ttgtaatctt tcagcatctt tttttcgctg tccgagatga
    61501 gcgtgaatgg caattggtgt tttgttttaa acttttgatg agaagcttgc gaatcggcac
    61561 ttacaccaat aactacaatt ccatttttag ttaaaatatc gtaattgtcc ctcaagctgc
    61621 atgcttcagc ggtacatcca ggagtgtcat ctttggggta aaaataaagc gccagttttt
    61681 tacctttaaa atctgccagt gtcacctttt ttccgtcctg gtcaacgcct tcaaaaaaag
    61741 gagctggttg gcctgttttt atttcactca tattctgata aatgtaattt tgtttagaat
    61801 gttagatata tttgcagcat taacaatgaa gcagttaata ttgtttaata tttttcaaaa
    61861 taagttcatc agcttgggca agtatatttt tcagggttgt ataattgcaa tgttgctatt
    61921 tataattacg ccttcttggg ggcaacagca aaggtcttct caagcccaga cggctataca
    61981 gttgtttgat ggtgggcaat atactgaagc tttgccttta tttaataatc ttgtaaataa
    62041 atacccgaaa gataaccttt ataaaaaata tcttggggcc tgtattgtag aaacaaacgg
    62101 cacactttcg caggctgtaa actatcttga aatgtataag gtgggcgaga agctgtattg
    62161 gcctgaattg tacctgataa aagcctattt acgcctgttt aaagttgaag aagcccgaaa
    62221 agtccttgaa tcattaaaaa gagatatgcc ttattttgaa gcccgtaaat atgggcttgc
    62281 tgaaatggaa cgctatttaa acttcttgaa ttttaatatt tcagaaaacg aatatggcca
    62341 agtagctgaa aaagtattac taaatattga tatcctaaaa aataatgcag aggcatggtt
    62401 tgatttaaaa tatcttaaaa ttggcgcaga cagtttttgt ttggtgttgg tgaaaaatga
    62461 agagttacag ccaaatacta cttatttgtt aaggaaaatg ccttcaaaag ataatgcgct
    62521 ttacatgagc cgtttttcgg cttttgggca atggcagcaa cccgaaaaat ttgatgctgc
    62581 ttttgaagga tttaccattt caaatgtttt tcctccttat attgaccagc aaaaacatgt
    62641 agcttatttt tctttagaaa aaaaaagcca gccaactggt tttgatattt atatggccga
    62701 gtttactccg aaaaatctga aacaagtaaa attaaaaata ttgccattac ctgttaattc
    62761 tccgtggaac gagtatttat acatttcgtt tgatacgctg tcgcttttag tgtcagatcg
    62821 tgaaacgtct ccccaaaagg ctgtgcaata tcttttgcaa aagcaaacaa agatagacct
    62881 tgcgaatttt aaatcttacg agttgtataa tttttgtttt ttcaaaaaat caatcactaa
    62941 aatttcttta ccgcaacaaa aaattgaaac atcaaaagtt ttaccttcat catatcaaga
    63001 tgaagagatg gttacacagg ctttagaaaa acagcgcatc gcagattctg ccatgtttgt
    63061 tgcatatcag ataaaaataa agattgctaa taccgaagat aagaaaatgc gaacaacgtt
    63121 gtttggagaa atgaaaaaag ccgagaaacg tgcaaaaact ttacaagacg aagccaatgg
    63181 tatgtatcag caatttatcc ctgcattgtc tgctaaaagt caggaaatta ataagttttc
    63241 agaagtgatg tcaaaatcaa aaaatagcga aggcgatgta gttacaaagt ctgatggaag
    63301 tttggtttct tatcgtattc aactcgggtt attttctaat cccaaaccgg cagttttttt
    63361 tgccggcatt gattctgtta cctctgaaaa tgttgataat ggaaaggcat taagatattt
    63421 tacaccttac tatgggacct atccagatgc tgacagtatg ctaactattg taaaaagtaa
    63481 agggtttaaa gaagctttta ttgtggccta ttacaataaa cgcaaaatac cacttgaacg
    63541 cgcgcggcag atagagaagg agtgatttac ttatttttaa catttgctat taacaaaaat
    63601 catgattgtt gttaataact tgtcgtcaaa atacattaaa tttgatatac aaattgaaat
    63661 tagcttacta attttaattt catgttttaa ttgcatagaa aatgaaaaag tttgatgaat
    63721 ttagtcacag ccctcaattt gatgaagata tcgagaagac gaacgggctg gccaattttc
    63781 ttacccttca taatgatgat ttgcatactt ttgaatatgt catagaatca ttagtagagg
    63841 tttgcaagca tgattttgag caagctgaac aatgtactta tttggtacat tataaaggta
    63901 tgtgtgatgt aaaaaaaggt agttatcatt cattgatccc ttataaaaaa gagctgtccc
    63961 gtcgtggttt aaaagttacc attaactgat agttgctgat gaccatactt accattgtcc
    64021 ttcttgttgc attagcaata tttttgctgg ttcttgaatt atttattata cccggtatta
    64081 gtattgccgg cattggtgca atactcgttt atgcaggagc gattgctatt gcttatatca
    64141 agttaggtgt ggttggggga aacattaccc tggctgcttc tattattagt tctgccatag
    64201 cagtttatat tttctttaag cccagtactt ggaagcgagt tacattgtca accgaaatta
    64261 cagccaaagt accagagtat gaagtttgtt cccttcttaa accaggtatg cagggtgtgg
    64321 ctaaaacccg gcttgggcct atgggcgaga tagaaataaa cgatattatt accgaagctc
    64381 aatcaatggg agcaattatc aatgctaata ctcctattga aattgtttat atagaggatc
    64441 ataaaatttt cgtaaaacct ttaatctgaa atactatgga aataggaatt tatttgtttg
    64501 tcggtgccat tggtattgta ctattatgga tattctttta ccttgtccca atcggccttt
    64561 ggtttcaggg gatacttacc ggtgtaaaag tttctttgtt gcagttaatc tttatgcgat
    64621 ggagaagggt tccgccttct gtcattgtta atgccatgat aaactccaaa aaagcaggcc
    64681 ttgatttaag tccagaccag ctagaggctc actttttggc aggaggtcat gtgcaaaaag
    64741 ttgtaaatgc acttatttca gctgacaaag caaatattcc gcttgatttt cgtgctgcaa
    64801 cagctattga ccttgcagga agagatgtat ttgaagcagt tcagatgtct gtcaatccta
    64861 aagtgattga tactcctcct gttacctctg tggcaaaaga cggtatccag ttgattacaa
    64921 aagcccgtgt aactgtacgt gctaatatca aacgtttggt tggtggtgct ggtgaagaaa
    64981 ctgttcttgc ccgtgtaggt gaaggtattg tttcatcaat aggctcatct gcttcacata
    65041 aagaagtact tgaaaatcct gataaaattt caaaaacagt gttagccaaa ggccttgatg
    65101 ccggaaccgc atttgaaatt ctgtctattg atattgcaga tatagatata ggtaaaaaca
    65161 ttggggctgt attgcaaatg gatcaggcta atgctgataa aaatattgct caggccaaag
    65221 ctgaggaaag acgcgctatg gctgttgccc ttgaacagga aatgaaagcc aaatctgttg
    65281 aagcccgtgc aaatgttatc caggctgaag ctgaaatccc taaagccttt gcagatgctt
    65341 tacgctctgg taatatcggt gtaatggatt attacaaaat taaaaatata gaagccgata
    65401 ccaatatgcg cgatagtatt ggaaaatcag gcattcagga agacccatca aagaaaaaac
    65461 aataaaagtt tctgaatttt ttgccagttt cggttgtttt tatacttttg tggcgcttta
    65521 aaatttaagg cttgttcggt gaggtgggtg agtggcttaa accaacagtt tgctaaactg
    65581 tcgtacgggt aaccgtaccg ggggttcgaa tccccccctc accgcaaaaa tactatcaag
    65641 agagcaacca atcggttgct ttttttttgc tttatttttt ttaatacgta tttatactac
    65701 cgtcatacta ctgcctaaaa tgtagtttaa tgccctattt catttcatag ttgaacgatt
    65761 tacttttggt gatagctaaa ctaatgactt tacattatgg gggcactata cgacgaactt
    65821 gctaaagata tacatcttca ggaaggacta aatgcttgta ttaactgcgg tacatgtact
    65881 gcaatatgcc ctgctgctgg cttttataat tacgaccccc gccagatagc cgatattgtg
    65941 cagcagcaag atgataagca aattgaagaa ctgctgaaaa gcgagacgat atggtattgc
    66001 ggcgaatgta tgagttgcaa aactcgttgt ccgcgtaaca acactcccgg gcaaataatc
    66061 attgccctta gaagttatgc tcaggaacat ggctatttta ctcaatcaga aaaaggccgt
    66121 cagcagatat atttaaaaag aactgttgga caatggattc ttgatcatgg ctattgctta
    66181 tatcttgaag gtgtaggaac tgacaatcat cctgagcaag ggaccatctg ggattggcgt
    66241 caggataact ggacagaaac aatggacagg ttgggagcca attatcagaa ggccggggct
    66301 ggtgctatgc gtaagattcc ggaagaggcc ctggaagaag tgcgaaaaat attcgaagta
    66361 actggaggaa tggaacgctt tcgtaagatt gaagagtatt ctgcacagaa agccagtgaa
    66421 atgggcctcg aagacgagga atatgttaaa gaagtatata caaccaatag cagtcaagat
    66481 aataaatagc cagataagat gaaagtagaa ggaaaagccc ggatctggaa aaattatcag
    66541 aaagaaatac ctgataacga tttcttttat gttcgcagtt gtgtacgcca gaacttcttt
    66601 cccggtgcgg agactacttt tttgcgtatt atgaaagatg agctgggtat taatgtatat
    66661 gacgatcctg cccatacttc atgcactggc attggatacc atgcagatat tgttcctttc
    66721 gatacgacca tgaccgtggt ggccagacag tttgccctga tgaccgaaaa aggttttaag
    66781 aatatgattc cttcctgcat tacttcattt gggctttata ccgaaattct tgaaacctgg
    66841 gaacaccatc ctgaagtgtt ggaaaaagcc cgtaaatacc tgaaggaagc tacaggacgc
    66901 acttttgaga tacctgaaaa cctttcgcat gccagcgacg tgatttttaa atttcgtcat
    66961 aaaatagctg agagagctaa atataaactg ataaacaaat atacaggcaa gccattgcgt
    67021 gtggttgaac atataggatg ccattatgct aagatgttcc cgagtaaggg agttggagga
    67081 gctgaatatc cttatgtttt gacaggtatg gtggaatctt ttggtggtga aatagttgat
    67141 tatcctgaac gtcgcagttg ctgtggcttt gggttcaggc aatacctggt gatggccaac
    67201 aggggttata ctattgccaa tacaaagaag aagtttgatt caatggatcc ttataaaccg
    67261 gactgtatag tgacaaactg tcctgggtgt cccatgtttc ttgataaagg gcaatatgcc
    67321 atagcggaac ttgaaggccg tgtgtatggt gacggaaagt atggcatacc agtacttaca
    67381 tttgaagaac ttgcaggtat tgttttgggg tatgacccat gggatatagg attgcaggta
    67441 caccaaattc cggtagaacc attattggat aaaattggag tggaatacga taaaagtgcc
    67501 aaatataaag gtattggtgg tagagattta ggaaaaccac agcattctaa gctgattaaa
    67561 gcatatatag aataattatg gctgaaagca aaaaaatagc agtaattggc ggaggcattg
    67621 ccggaatgga agctgccggg aaccttgtta accttggttt tgaagtctat cttatagaaa
    67681 ggaccaacag gcttggcggc aatgtggcaa attggtattg ccttttcccg gattttaaac
    67741 catcatcaaa ggttgttgaa caattaaaac ctgctattca gaacaaagtg cagtttcgtt
    67801 ttggagccga ggtttgtaaa accgagaaaa aagggaacaa gcatttactg catcttaatg
    67861 atggagaggt gctagaatgt gatgctgttg ttgttgcttc cggtttttca ctttttgatg
    67921 cccatcgcaa agaggagtat ggatatggga tttacgacaa tgtaattact tcggccgatt
    67981 tggaaaagca acttgccgaa aaccacacag taatgacaac tcagcataag gcacccagaa
    68041 aaatagcctt tatacactgt gttggttcac gcgatcataa agttggaaat gcttattgtt
    68101 ctaaggtttg ctgtgttact ggtgtaaaac aggctataga agtggcaaaa atgcatcctg
    68161 aagccgaaat atattcattt tatatggatt tgcgcatgta tgaccgtcag tttgaggatt
    68221 tatacatgga agcacagact caacataatg taaggttcat caggggaagg ctttcggagt
    68281 gttttgaaaa tgccgataag acgattcttc ttaaagctga agacaccttg agtggaaagc
    68341 cttttcggtt aagtgtagat atggttgttt tgctcgtagg tatgcaggca gaccaatctc
    68401 tgaagcatat ttcggacatg tttgcattga aaacttcgga tgataggttt ctgagtgtag
    68461 ccgatgttca tacttcttca atatattcgg gtgtagaagg tgtttatctg gcaggagcat
    68521 gtacagggcc aaaatcgata catgaaacgc tgaatagtgc tcgtgcagct gcattaagca
    68581 cagctatata tctgaatcaa aattaatagc aatgaaaaac tggggctatt ctatacataa
    68641 agacaaccag atagaccttg atcggaacga tacaagtgta tataaggcat tgattcttgt
    68701 agaaccttca atagcccgtt gcatatcatg tgggtcatgt tgcgctacat gtagctcagg
    68761 agaatatacc agtttgagct tcaggaaaat tatgattcag atttcacgag gtgaaacaaa
    68821 agatttgtat tcaaaccttt ctcaatgtat gctttgtggg aagtgcatcc ttgtttgccc
    68881 ccgtgatgtc aatacccgca atgccataac aaagcttctt caaatattaa ccacaaaaaa
    68941 ctaaagctat gttgtttgat tggtttgtca tacctttttc aattggatta gtttttgtat
    69001 tgggttatac cattgccaaa tatattagct gggttcatca gcttgatcct aaagataaaa
    69061 aggccttttt tgcccatttg ttttctaagg gtatattttt ttctatcaaa gaaatctttt
    69121 cagaatcgct tctgcaccga aagattttta aggtaaacaa gaagttgggc tttatgcaca
    69181 tgagcttagc ttttggatgg tttttgctta ttgcccttgg taatatcgag attaaatttt
    69241 atgctgaata tgctattaat ccgccgtatg tacctatttt tttaaggttt tttcagcctg
    69301 atccggctcc acacttcttt gggcgtggtg ctaacttcct catggacttg ttgctcctta
    69361 tgatacttgt tgctgttggt ttggcaatat acaaaaggtt tcgccccgga ttttttggga
    69421 tgaagaaaac tacggtgctt catgttcctg ataaaatagc cctttacgca ttgtggtgta
    69481 tctttcccct tcgttttttt gccgagagct ttacttctgg ggtttataac aatggtagct
    69541 ttcttaccgg ctctgcaggt tccttttttg cagcattttt gccagttgaa aacttgtatt
    69601 atccggcctg gtgggcctat tcttttgctt tgggtacatt ctttttgttt ttgccttttt
    69661 cgaggtatat gcatatcctt gcagaggtta tgcttattgt aatgcgcaat gcaggtgtta
    69721 aggcttgtat taaaaacgat gtttaccatc aggtgcaaat aatttcgtgt tcgcgatgtg
    69781 gcgtttgtat tgataaatgc cagctttcga aagttaatga ttatgcaata cagccatcat
    69841 actttattcg tgaggtcagg tataacaagc tacaacaaac aaccacagaa aactgtttga
    69901 tgtgtagccg atgcgacagt atttgcccgg ttggggttga atctgtagat atcaggctga
    69961 gaaaaagggc agagggtaat aatattacat ctatgggcta tgaaaaaata aaacctgttg
    70021 acctgcctca taaaagtatt gatattctct attttgcagg ctgcatgagc catcttacac
    70081 cagctgttaa agcttctatg aagaagattt taaaagcctc aggacagacg tggcaaatga
    70141 tggatgaaaa tgctaccatc tgttgtggaa ggccacttca attgaatgga cagtttgaag
    70201 ctgcacaaaa tcttagagat aaaaatgcca agctgataaa acaaagcggg gcaaagcttt
    70261 tggttacttc ttgtcctatt tgttttaaga cttttaaaga tgattataac ctcagtattc
    70321 aggtagttca tcatacccaa tatattgcat cgcttattga taaaaatgtt ttgccaattg
    70381 aacaaactga tggccgattc tcctattatc atcatccatg cgaattgggc agaaatagtg
    70441 gggtttatga agaaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    70501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaag
    70561 agtaaatttg acacttatta ataacaaact aaacagaaaa tttcggcaat gttagattgt
    70621 attgttttag cgtttatttt ttgagatttt ggttagaatt tgtttttggg tcctttaaag
    70681 aatatatgcg aagataaggt gtttaacatg tcaaattttt tatgaataaa acaactaata
    70741 catttaattt atgaattgga cttctcatga atttatttgg ctcgactggc ttattattgc
    70801 cgtcggcatt ttggccattg catgggccgt ttggcgttca atacaaaaac acaagcgtac
    70861 tttagcagga gccagtagtg aagattacct ttttggtaaa ggagaaccat ggtatattat
    70921 tggtgcagct atttttgcgg caaatattgg ttctgaacac cttgtaggac ttgctggtac
    70981 aggtgcaaag tctggagtag gaatggccca ctgggaaatg caaggttgga tgatacttat
    71041 tttgggttgg ctttttgttc ctttttacca attgatgaac aataagttgg gtaaaattat
    71101 taccatgcct gattttttaa aaacccgtta tacacatcgt acaggttcat ggctgtctat
    71161 tattacacta atagcttacg tgctaactaa agtaagtgta acagcgttta ctggtggtat
    71221 ttttatggaa agccttcttg gtttgccttt ctggtatggg gctattggat tgattgtttt
    71281 aacaggtatt ttcactgttg ttgggggaat gaaaggggta atgactctat cggccataca
    71341 aacacctatt cttatcctgg gttcttttct tgttctcttt ttgggattag cagctcttgg
    71401 agacggcagt gttgttcatg gctggacagc aatgattgaa catgccaaaa cactcaataa
    71461 tggatatgga acaaaccaca tgttccattt tgaaacagga gacccgatgt atgacgatta
    71521 tccaggtttt tgggtattta ttggcgcttc aattattggt ttatggtatt gggccacaga
    71581 ccagcatatt gttcaaaggg tacttggcca atcaaaaggc gagcctaacg atattgtgat
    71641 gaaacgtgcg cgcaggggca caatcgctgc tggttatttc aaattattgc ctgtgtttat
    71701 gttcctcatt cctggtatgg ttgctgcagc cttagctgcg cgtcctggca gtggttttac
    71761 attagaaaac cctgatacag cctttggttc aatggttaaa tttgttttgc ctgctggtgt
    71821 gaaaggtatt gtgaccattg gttttatttc tgctttggtg gcttctttgg ctgcattctt
    71881 caactcttgt gctacacttt atactgaaga cttttataaa ccaatgttca aaggtaagac
    71941 agaagaaaga tatgttttgg taggccgtat tgcaactgtg gttgtggtaa tacttggcat
    72001 tgtttggatt cctgtaatga tgagccttgg cagtctttat ttttatctcc agcgtattca
    72061 atcattgttg gcgccagcta tggtagctgt ctttctctta ggtatttttt caaagagaat
    72121 tactccaaaa gcaggtgagc ttggtttgat agttggcttt ttcattggaa tgatacgctt
    72181 actaaccaac attcttacca ataccggaag caatgtaatg acaggctggt attgggaagc
    72241 tactgcatgg ttctggcaaa ccaactggct tatttttgag atttggttgc tcgtctttat
    72301 catgctgttt atggttgcag tttcattctt cactccaaag ccaacgcctc agcagataga
    72361 cgctataact ttcactagtg actacaaaga acagatcaga aagagctgga acaaatggga
    72421 tgtaattgcc tcattagggg taatactctt ctgtgcatta ttctactatt atttctggta
    72481 aaaaataaat tagctaattg gtgaatttgc aaattagtaa attcgttaat tagctaattt
    72541 ttaaaacatt aaaggaccct tgcgttatta attttcttga ctcttgattc taatataaaa
    72601 taaaataaaa aatgaaagct tttgaaaatt ttgaagtatg gtttgttaca ggggctcagc
    72661 ttctgtacgg tggcgatgca gtagttgctg ttgatgccca ctcaaatgaa atggttaaag
    72721 ggttaaacga ttctggtaaa cttcctgtaa aagtggttta caaagggaca gtaaactctt
    72781 ctgctgaagt tagtgctact tttaaggcag ctaataacga taaaaaatgt atcggggtga
    72841 ttacctggat gcatactttc tctcctgcta aaatgtggat tcatggtttg caagagctca
    72901 aaaaacctat gctacacctg catacccagt ttaataaaga aattccatgg gatactatgg
    72961 atatggactt tatgaacctt aaccagagtg ctcatggtga ccgtgaattt ggctttatca
    73021 atgcacgtat gcgccgtcca cgtaaagtgg ttgttggata ctggcaggat gctaatactt
    73081 tggcccgcat tgcagtttgg atgcgtgttg cagctgcatg ggctgatgcc caggatatgg
    73141 ttgttgttcg ttttggtgat aatatgaaca acgtagctgt tactgatggt gacaaagttg
    73201 aagccgaaat gcgcctgggg tatcatgtag atgaatatcc tattggaaaa ttggttgctg
    73261 taatgaacaa agttactgag gccgagattg ctgctatgtt aaaaacatat gaagccgaat
    73321 atatccttgc tgataatgtt aaagaagggg gcaaagatcg ccaacgcctt gttgaggccg
    73381 ctcgtgaaga gattgctatt cgtaagttcc tcgaatcaaa gggggcaaaa gcttttacta
    73441 caagctttga cgaattggaa ggtttagatc agcttccagg tcttgcttct caacgcctga
    73501 tggccgaagg atatggtttt ggcgctgaag gtgactggaa aactgctgca ttgtgccgta
    73561 ccatgtggtt tatgggccag gggctcgaag gtgggtgctc tttccttgaa gattatacct
    73621 taaactttga tggagagaag agttcaatcc ttcaagccca catgttagag gtttgccctc
    73681 ttatagctga aaataaacct cgattagaag ttcatccatt aggcattggt ggaaagaatg
    73741 accctgctcg tcttgttttt actacaaaac caggcactgg tgttgctgca actattgtag
    73801 atatgggcaa ccgttttcgt atgattgtca accaagtaga ttgtattaaa tcaaaagcgc
    73861 tccctaaatt gcctgtagct tctgcattgt ggattccaca acctaacttt gaaattggtg
    73921 ctggtgcctg gattttggct ggtggtacac accactcaag cttctcattt gcccttacac
    73981 aagagtatct tgaagattat gccgaaattg ctggcattga gcttgttgtt attgataaag
    74041 atactaccat tagcaacttc aagaaagaac ttcgtatgaa cgatgtttat tacatgctaa
    74101 acaaagcact tagctaatat aaaacatgga gccacggtga cacagagaag tacagtaaaa
    74161 agctttatgt aactctgtgt ttttgtgcct ctgtcttaaa aaattctttt tatgcttgaa
    74221 gaattaaaaa agaaagtttt tgaagccaac ctggagttgg tgcgccaaaa gttggttatt
    74281 tatacctggg gaaatgtaag cggtatcgac cgttcacaaa acctcgtggt gataaagccc
    74341 agcggagtgt cttatgacga tatgaaagct gaagatatgg taattgttga ctttgatgga
    74401 aatgtggttg aaggtaagct tcgcccatct tcagatacag ctactcatat tgagctatac
    74461 aaagctttct ctggattagg cggtgttgtc catacccatt ctacttatgc agtagcttgg
    74521 gcgcaagctc aaaaagctat tcctgcattt ggtaccaccc atgctgacca tttttacggg
    74581 gctgtgcctt gcacccgtcc tcttacaaaa gcagaagtag aaaacaacta cgaactgaat
    74641 accggtaaag taattgtaga aacctttgcc aaaatagagc cattaagtgt cccttctgtt
    74701 gttgtggctg gtcatggccc gttttcgtgg ggcgaaaccc ctgaaaaggc tgtttataat
    74761 gcggttgtat tagaagaagt agcaaaaatg gctatgatta caacccgttt aggcggagaa
    74821 aaacctatcg accagtattt gcttgataaa cattataacc gtaagcacgg gaagaatgct
    74881 tattatggac aaaaaaaatg agtaaatgag agaatttgag aattagcaaa tggtcaatat
    74941 ttgtcttgat tcttttctct tgattccaga atctaaaaaa taaaaacatg tcaaaataca
    75001 cattaggact tgattatggc tctgactcca tgcgtgtggt cattgccaat gttgaaaatg
    75061 gccaagtagt tggaactgcc gtttcagaat atgcccgctg gaaggctggc aaatattgtg
    75121 ttcctgcaaa gaatcaattt cgtcagcatc ctcaggatta tatcgacagc atggaacaag
    75181 ctgttaaaga agctctttcc aaagctcctg ccggtacagc aaaagatatt attgctattt
    75241 ctatagatac tacaggttca acgcctgttg ctgttgataa gaatggtacg ccactttctt
    75301 tgttgcccga atttgctgaa aatcccaatg ccatgtttgt gctttggaaa gatcatactg
    75361 cagttaaaga agctgaagaa ataaacaacc ttgcccgcaa gtggaagatt gactttacaa
    75421 aatacgaagg cggaatttat tcttcagagt ggttttgggc aaagttactt catattatgc
    75481 gtgaagatga gaaagtcaga gcagcagcat actcgtgggt tgagcattgc gattggctcc
    75541 cggcattgtt gacaggtaat acaaagcctg aaaaccttaa acgcagtcgt tgtgcatctg
    75601 gtcacaaagc tatgtgggcc gaagaatttg gaggtttgcc tcatgaagat tttttggttg
    75661 cattagatcc tgtacttaaa ggtatgcgcg agcgcctctt tagcgaaaat acttatactt
    75721 gtgatgtaaa agttggtaca attgctcctg aatgggcaaa acgtcttggt ttgtctgaaa
    75781 atgttgctgt tggtgttggt gcatttgatg cacaccttgg ggctgttggc gctcagatta
    75841 aaccatatca gcttatcaag gtaatgggta cttctacatg cgatatgctt attgctcccg
    75901 agaaagatgt tcatgggaag ctggtaaaag gcatttgcgg ccaggtggac ggttctatta
    75961 ttcctggtat gattggctta gaggctggac aatcagcttt tggcgatgta tatgcttggt
    76021 ttaagcgcgt agttgtagag ccggctgcta aattgattga gcaatcttct gccatttctg
    76081 ttgatcagaa agctaaacta attcaggagc ttgaaaataa gcttattcca ttattgtcag
    76141 atgaagcggc aaaaattgat cctgctgata ctactgtaat agcattagat tggatgaatg
    76201 gtcgtcggac ccctgatgcc aatcagatgc tgaaaggtgc tattaccggc atttctttgg
    76261 gctcagatgc tgcccgtatt ttcagggccc ttgtagaatc tactgctttt ggagctaaga
    76321 agattgttga ccgttttatt tctgaaggtg tgaagattga tggcgttatt gccattggag
    76381 gagttgccaa aaaatcgcca tttgttatgc aaactatggc agatgtgcta aatatgccta
    76441 ttcaggtagt agtttcagat caggcatgtg ccttagggtc tgctatggca gcagccgttg
    76501 ttggaggggc atacaaaact gttgaagagg ctcaggaagc tatgggtagt ggttttgaaa
    76561 ctacctatca tcctaatgcc aaaatggttg aggtgtataa agatatttat gcacgttact
    76621 ctttgctggc tgaaacatta gaacctcttt ataatgctca gaaaatatag aaatttgtaa
    76681 gttttattaa agaaacgctt tgcatttttt taatgttaag cgtttcttgt ttttatttgt
    76741 ttgtgctgtt aaaaatacat ttttatattc tccctcttta taatgttaat ttcaaatcga
    76801 ttaaacattt ctaaatgctt aatttgggtt aatatatttt ataattattt taacgttaga
    76861 gataattact gaattggttt tgagaaataa aaagtgtctt tttgtatgta aaggttttgt
    76921 tataaatatc ttagtgtttt ttcatatttt taccagacaa caataataaa aatatatttt
    76981 tttatcagaa aaatatatat ctttgcagcg ctttaaacaa agaagttttt aaatcagtct
    77041 ttcgataaat gtaaatcgtt gggcttgtaa acaaaaatat attgcgggat ggagcagttg
    77101 gtagctcgtc gggctcataa cccgaaggtc gtaggttcga gtcctgctcc cgctactgtc
    77161 aaggataaat tggtgtcaat gctggtttgt ccttttttta ttttatccaa tatctgagga
    77221 gttttttaat cagccaataa aaaaagctgc cattgctgac agctttaact catctttcga
    77281 cgaatttttt taaacaccac ctatgaatga ttctaaaatt aaattttagt tatgcaaata
    77341 tactatttag ttttaaataa aaaacttatt tttaaataaa agtgtcaaaa tgtccatttc
    77401 cttagtacta taaatacagg taagtgttga agttacaatt tctaataacc ggataaatgc
    77461 ttttgtacat ttattcaaca aatgagaaca tgtgtataaa aaatgtttgt caataaaacg
    77521 taattttgac tcttataaaa accagataaa atgaccaaca aaataaaaac catatcatgt
    77581 tgtttagtaa tattggcagg ggtaaatttt ttagccaaag cgatagacaa agcttctgca
    77641 agctatcatg acatggctac agaacatttg tttcaaaatt taaaagcttt aggcgatgcg
    77701 gggttacatt tgtttggagt ggccaatgct tttgatatca gttataaaaa gcttattcac
    77761 gagtatgatg gttcgacaga ctgcaaagat attaccggtt cgcatccggc ttttatcgaa
    77821 agcgactttg aatggtatat tcacaaagag cataaagcaa aatgggctac agaagccatt
    77881 cgcgaagcat acaaacaggg gattgttgtg ggttactgtt atcacctgcg cggactaaag
    77941 tctaatgatt tttacgcttt taaagacaat aaaaagactg aagattcgac ccttttgcaa
    78001 gatattgttg ccaaccctga caggaaggcc aacaaagcat tagactggta tttgcacagg
    78061 cttgattctg ttgttatccc aacttttaaa gaattagggt tcccgctaat ttaccgtcca
    78121 ttccatgaga tgaccggagg atggttctgg tggggtacaa gttgttgcac taaagatgaa
    78181 tttataaaaa tataccgtct taccgtggac tatttgcgca gcaacggcgt tagaaatgtg
    78241 ctttttgcct ggtcgcctga taaaagcagc gacagtactt tttatcctgg agatgaatat
    78301 gtggacatca ttggttacga tggctacgat gtagggcttg ttgattacaa ccaaattccg
    78361 gcatttgttt cgaatgttgc agcattggct gattttgcac aaagacacaa taagatagtg
    78421 gctattaccg aaacaggcac aggcaaactt ttggaagctc ctgaatactg gacaaaaaac
    78481 atacttgaat cattaaatgc agacccaaaa gcttctaaag tatcatggat aatgacttgg
    78541 tatagtgcag actggaacaa agacaataag ggggtcaatt atatccctta caaaggtatt
    78601 gaaaagaaaa ccaatgggga caaagctatt gatgacttca agaagtttta taacgaccct
    78661 gccacagtat ttcaggttga ctcgttgccg ctttttgcaa cagacaacgc tgaacttttt
    78721 atttatccta aaaatctcac tctaacagtc ggtaaaaagt ttacgctgat tggaggtaga
    78781 aaaaaagact ggtctaaaaa ccaggtttca tggttatcgt cagatccttc agttgtaaaa
    78841 gtagatgcga agggaaatat agaagcttta aaaacaggca acaccaccat tactataaag
    78901 atgggcaaca aacaagccag ttgtattgta gttgttcaat aatcatgaaa aaaccttcca
    78961 ggtttttaaa acctggaagg tttagttata acaaaatctc atttacttca ggtttactaa
    79021 agcagtgcta acattctttt ctatacgtgc aataatatca gtattatcag cctttgcaaa
    79081 cttatctccg gtaatttttt caaacagttc aatatatctg tcgctaactt gagctacaaa
    79141 ctcttctgac atattaggca ctgtctggcc ttccttgcct tggaagttgt tttcaatcag
    79201 ccattggcga acaaactctt ttgaaagttg tttttgggct tcgccctttg ccagtctgtc
    79261 ttcataaccc cgggcataaa aatatcgtga tgagtcggga gtgtgaatct cgtcaataag
    79321 catgatttgg ccgtcttttt tgccaaattc gtatttggta tctacaagta tcagtcccat
    79381 tttggcagcc atttctgttc cacgctcaaa tagcttgtag gtgtattctt ccaacttaga
    79441 gtaatcagct tcagatacaa tgccctgggc aataatttct tcgcgggaaa tatcgaggtc
    79501 gtggccttca gatgcttttg tagtaggcgt gatgataggt tttgggaact tttggttttc
    79561 tttcatcccg tctggtaaag taacaccgca taaggtgcgc ttgccggcag aatattcgcg
    79621 ccatgcatgg cctgtaagat aaccacggat aaccatttct actttaaatg gctcagcaaa
    79681 atgcccaata gtaaccattg ggtctggtga ggcaattttc cagtttggaa caatatcagt
    79741 agtggcatca aggaatttaa cagcaatttg gttaagtacc tggcctttgt aaggaattcc
    79801 tttaggtaac actacgtcga aagctgaaat acggtctgaa acaaccatca cgaggtattt
    79861 acctccaatg ttgtacacat cgcgaacttt gccggtatat ttgccggtct gtcctgcaaa
    79921 atgaaaatct gttttcgtta ttgcctgcat agttaaattt tttgctcaaa attatgaaaa
    79981 aggaaccaaa tttattttgt ccaataaaca caaaaatccc tgcgatggtt cattttactg
    80041 aattgctggc agggattaaa tttggattaa atttaggaga ggttaggagg gggctttatt
    80101 acttttttac caaaatcaaa aactcccaag gtgcaagctg cattttttta tactggtgca
    80161 attcaatgtt tttgccagaa aatgcctcgg tgtaattgcc tgaaatataa ctttctattg
    80221 caaaattttt ggttttggat gtaaagttga aaataccaat cacggtatta gtctcttttg
    80281 tccgttgaat agccatcacg tgtttgttgt ctgtcaggtt tatgcgctgc agtgagccgc
    80341 cagcaaaacc attccaaagg gctttgttat ctttcttaat agaaataagc ttttggtaaa
    80401 aagaagtcaa tggataatct ttccaatcaa tgctatcctt ctcgaaaaat ttcaaacgcc
    80461 tgttaaaacc agattcctga ccggtatata tcaatggcat accagggaca atgaaggtaa
    80521 gtgcagcaaa tgttttggct ccatcgccca tacgctcgta ttcagtgcca ttccatgagt
    80581 tttcatcatg attggaagta aactgcatca ggatagaacc ttgaggatat gcagtatcca
    80641 cccaattgaa atgctttgta atatcattgg cattcttctt tcctttagct gtctggttca
    80701 tgatatgatg aaacttccag tcgtaactgg catcaaaagc attagcatgc aggaaaggct
    80761 ggtcagcttc agcaaggaaa aagatgagct tggttttgtc caattcaata cggcatgaat
    80821 cccagaaact taccgggacc atgtgtgcca catcgcaacg gaagccatct atgtcggttt
    80881 cagtaagcca aaatttcatt gtctcaatca tccatttgcg catatcggca ttgttgtaat
    80941 caaaacggac aacatcagtc cagtcaaagg gagataccat tttacctgta gaatctttca
    81001 ggtaatactc cggatgttcc tttatcattg ggttatccca cgatgaatgg ttggggaccc
    81061 agtcaattat gattttcatg ccctgctcat gcactttttt aaccaggttt ttaaaatcgt
    81121 ccatgttgcc aaattctgga ttgacagctt tgtaatcctg cactgaataa taactgccaa
    81181 gaggcccttt tctgtttagt ttccctatag ggtttatcgg catcagccaa aggatatcaa
    81241 tgccaaggct ttttagacgt ggcaaatctt ttgcaaaagc attaaaagtc ccttcctttg
    81301 aatactgcct gacattcacc tcataaataa cagcattttt aacccactcg aggtttttga
    81361 cagtcgtagg agccggtttt accttctctg gagttgtgcg ggtacatgca aaagctacca
    81421 ataccagcat tacaatcatt ccaagtatta gaattgacgg atgtttcatg acgataaaat
    81481 ttagttttta tcttttagtt caataatgat agcagtttta ggagcaacag aaaaatcaga
    81541 aaggttatat tcttcgcctg taattacatt ttttgccgaa gctgcacctt tgagcaactc
    81601 agcataaact tttgaatcta cttttacagg ctttttgtta ttattgacaa acacgacaat
    81661 ggcatcgtca tttttagcta ttcgcgaata aacgtacaat ccattattag gtacaaaatg
    81721 aacaagcctg cctttggcta tttcaggatt atttttacgc caggtaaata tttgacgggt
    81781 aaaatcaagc gcttcctttt gaatagccga taaaccatcg tttgtaaata cattgacctt
    81841 gtcacttggc cagccaccgg gcatatcttt acgcaaacgt ccgtcgcctt tagctttatc
    81901 tcccggaagc aaaatttcat cgccataata aaactgagga ataccacgaa gcgttgccaa
    81961 catggtaacc agcattttat acttatgaat atcgcctttt actgtttctt ccaaacggga
    82021 caagtcgtga tttgacaaaa atataaggtt ttgcatagca tcggcataca gaaagtccat
    82081 gcatagggtg ttgtacactt ttgccatgcc attatcccaa tcgtcactgt catttacaga
    82141 agctaacatg gcaaagcaaa gagggaagtc ggtaacatgt ccaatttgtg aagaataaga
    82201 ggatattggt ttttgatttc gttgaaaaca ggcagtaaaa gccggatacg aaaaccacga
    82261 ctctcctact atagaaaaat caggatattc tgcattgaca gctcctgccc agtcatttaa
    82321 aaattccata tcagaatagg gctgcgtgtc aagccttata ccatcgatgc tggaagtttc
    82381 aatccaccat aaagtattct gaataagata agttgccacc aaagggtttt tctggttcaa
    82441 gtcaggcatg gtattgtcga accagccctt ttcaaaaaca gctttatcat attgcgatgc
    82501 gtaagggtca atcaacgtag aagccctgta tgaggtacgt gtaaaggagt caaactggtg
    82561 ataccagtct tttgaaggca aatcattatg ccaccaatgc gaactgccgc aatgattaag
    82621 cacaaaatcc attaccactt tcaatttcat atcatgagct ttttttacaa agtcacaata
    82681 ttccttatta gacccaaagc ggggatctat tttataaaaa tctgtaatag cgtatccatg
    82741 ataggaagtc tgaggcatgt tattctcaag caaagggttt atccacactg tagtaaaacc
    82801 caattttgaa atatagccca gatgctggct aatgccggca atatctccac catgccttga
    82861 atccggtttc ctgcgatctg tagtgtctgt atatcctgca atgctgtcat ttttggggtt
    82921 gccattagca aagcgatcag gagtgataag gtacatagca tctgtagggt tgaaacctgc
    82981 cttaccgtcg gcttttgact tccgttctaa aaaagaatat tttaccacag tatctttttc
    83041 tcttccagaa aaggaaatag caatatcgcc agctttagct ttagatttaa cagacaaata
    83101 aacaaacaga taatttgcat tgtcagtttt ttcgatcttt tccaataatg caatactgtt
    83161 gtcggccagt ttaacattgc ttttagcaac attcttacca tacaccaaaa gtattagatg
    83221 ggggtttttc atcccaaccc accagttggc aggttctatg cgttcgatag atggttgtgc
    83281 atacatacct acaatgctta aagacattag tagtataaag gtcaggattt ttttagacat
    83341 ttggaagtat tttaatttta agcgtagaat tctatccacc cccaaccccc gccagcgggg
    83401 gacagctttt aatatttttc ttcttaatta ggcattatat caatagatgc aaatgttcaa
    83461 tttagaaagc cattttttaa ttgttatccc ccgctggcgg gggcaggggg tggacttcat
    83521 agttttaatt actttttttg ataatgcaac ttactattag cactaatagt gaaaggtgag
    83581 ccataaagca taatgctgat aggttccgga taatggttat gaatcaatac atcatgctga
    83641 gaaacaataa tttcgagcaa atggttacgg aactgaattt tgaacgaata cttattccac
    83701 ttttcaggga tcaatggctc aaaagacaat gtattgttga taatgcgctt gcgggcaaag
    83761 ccttccacaa tagccagcca ggttccggcc atggaggtaa tatgcaggcc ttcgtcaacc
    83821 tcgtggttat agtcgtccag gtcgagacga gaagtacgga ggtataattc ataggctttg
    83881 tctatatttc cgatatgtga agctataacg ctgtagatac ttgcagagag ggatgattcg
    83941 tgaacagtca tcggttcata ataattgaag ttgtcgcgaa tggtttcaac cgtataatcg
    84001 ttgataaaga agtacaatcc ctgaataaca tcgccctgct tgataaaaca acttctcaaa
    84061 atacggtccc atgaccaatg ttggtttata ggcctttgct cttttgggat actatctgct
    84121 gactttaatt ctttatccag aaacccgtct tgctgcaagc gaatgtttgt acccggaata
    84181 gaaggcaggt acatattttc tgcaatgtcg ttccaaagtt ttgtttcgtt tgcttcattg
    84241 aaatttatag atttaacaag ggcttcgtat tgttgaggaa aatctttggc caccatcttg
    84301 atgttttcca tggtatattt tagtgtccac ttcgccaatg tgttggtgta ccaattgttg
    84361 tttacattat tctcgtattc attaggcccg gttacaccta gtattacgta ctgcttctta
    84421 tcattggaga aattgacgcg ttgtgcccag aaccttgaaa gggcaataag tacctcaagc
    84481 ccatattctg caatataggc atagtcattc gtatgtaata tataattata tatagcataa
    84541 gctattgccc cattacggtg gatttcttcg aaggtaattt cccattcgtt gtgacattct
    84601 tcaccattga tggttaccat cgggtaaaga gctgctccat tggtaaatcc aagtttctgt
    84661 gcattttcaa tagctttttg gagctgtttg taacgataaa tgagcaggct tcttgctacc
    84721 tttgaatttg aagtgtttag ataaaatggg aaacaaaatg cttctgtatc ccaataggtg
    84781 cctccgccat atttctcgcc agtaaagcct tttgggccaa tattcaagcg ttcatcttct
    84841 ccggtatagg tttggtttaa ctgaaatata ttaaaacgaa ttccttgttg ggcttcatcg
    84901 tctccttcaa taacaatatc gcttttttcc cattttttct cccatgccag gcgatgagct
    84961 tcaaaaagct tgtcaaatcc ggtttgttcc atttccacaa cgtccttttt caactcagca
    85021 gccatagacg ctatatcaac atccagagat gttgtattgg caacgtattt gacaatagtc
    85081 aacgtttcat tttgcttcac aggaatatca aagctttggg caatgaactt ttccttttgc
    85141 tcaacagtag cagacactgg caatttcttg ccagaaacaa aaaagttcgt tttcatcgcc
    85201 gaacaaacat gaaaatccag cttcttggtt tttacctgaa gtgtggctac tccttcagtc
    85261 tcgcctttac ttacttcatc ccagaatttt tcgttgtagt ttgagtcttt gttttgaaca
    85321 tcagcatcta aaaagggttt aatcactgct tttcctgtaa agtttattgg cgttatgctg
    85381 tatgaaattg cagccatacg ggtattagca atactgataa agcgcttggc cgaaacctga
    85441 accatctttc ccgatggcat ttcagcataa aatgaacgtg aaagcagtcc ttccttcatg
    85501 tttaatacac gcttaaactg gctaactttg cattttgcaa ggtccagctg ctcgttttca
    85561 aaccagatat caatgccaat ccagtttgtt ccgtttatca tcttgccaaa gtattcagga
    85621 tagccatttt tccaccatcc aacgcgggtt ttgtcaggat agaaaacgcc tgcgaagtaa
    85681 ttgccctgca acgacccgcc tgaatagtgc tcttcaaagt tggccctgtg gcccatcttt
    85741 ccattaccaa tactgtagat gctttcagaa gcttcctgtc tttcggcatt aaactcttct
    85801 tcaatgatgc accacgggtc tgttttcaaa tatttgttca taggtatatt tttcattgtt
    85861 tggttataga atctaaggat aatactatat caaaattgag tttatcgagt gaaggaacaa
    85921 caatgtcggc cttcccaagc tgaacaggag acccaatacc aatacatttc atccctccac
    85981 ggatagcagc ttcgatacca gcttctgcat cttcaaaaac aacacattga cctggactaa
    86041 ccttcaaatc ataagcccca gtaagaaaaa cctgagggtc aggcttagct ttggcaatac
    86101 gtgtgccatc cacaatggtg tcaaaagcat gttcaagctt taatccttga aggatagaca
    86161 tggcattttt gctggcagag ccaatggctg ttttgatgtt atgacttttt aaaaactcca
    86221 gtatttctat tgctccagga agtatatctg ccggggtaag cgtagaaata tattctacgt
    86281 accacttgtt tttcatatca gcatattcca gcttcttttc gtgtgtaagg ttggtaatat
    86341 ttccaaccga taaaaggatt tctaacgaat ccatacgact aacgcccttc agcctttcat
    86401 tgtcgtgttc agtaaactca aaccctaaca tattcgccag ccttttccat gctatgtaat
    86461 gaaacctggc agtgtcaact attacgccat caaggtcaaa gatgcaagcc ttaatcatat
    86521 ttgttttaat taaatttttt aaaaattaat gagccttttc tttaataaat gaaacaaaaa
    86581 tgcttccgag caacattgaa acgccgcaaa ttataatcat gtaaatggct tgattattaa
    86641 atattgacct aagaataggc cctccaacta aacctgagat aatctgaggc ccggcaatgg
    86701 taaagttgaa aatgcccata tatactccca ttttttcggc aggcaaagcc cccgacaaca
    86761 atgaatatgg catagccaaa attgctgccc aggcaatccc aatgcctgcc attgatacaa
    86821 ataatgcatt tggcgtgttg aaaaaataaa ttgaaaggta gcccagccct ccaaataaca
    86881 gggctgatga ataggtcgcc tttcttccta ttttcttagc aatacgggtc ataaacaaag
    86941 aaaatatggc agcaaataag ctgtatgccc caaataaaac cccaacccag ttgccggctt
    87001 cctgatataa ctttgatgaa gaatcgccta tttcggtgcc aaatatatgc tgtgcaactg
    87061 ccggtgtggt atatacccac atcaggtaca atgcaaacca cgagaaaaac tgcactactg
    87121 ccaattttat cataaccgga ggcatttcgg caaaaagctt aaaaaacagc ttcaaccttt
    87181 ctgcaacaga ctttttctcc tttttcttgg acgaccatgc gtcatagtca atcccattct
    87241 gttcacaata ctcttttgga gggtattctt tagtacgaaa cactgtccac accacagata
    87301 atagaagtat tgccccgcct atataaaatg accagataac cgaaggaggc acatggcctt
    87361 cagcggcagt attctgaact ccaataatat tagtcaaaac ataaggcaat acagaaccta
    87421 ttacagcccc ggtgttaatc aaaaaacttt gaatggagta gcccaaggta cgttgttcat
    87481 ctgaaaccat atctgctacc aatgcacgaa acggctgcat ggtaatatta aacgagccat
    87541 ccatcagggc aaacataaca gccccaacag ccaaaactgg cataatagat accaacgcag
    87601 aagaattggg cataatccac atagccatac aagctattat agccccggca aggatatatg
    87661 gcgacctgcg ccccaggcgg ttccaagtcc tatcactggc agcccctact attggctgaa
    87721 ctatcagccc catgactggc gctgccaacc aaaagaaaga taatgaatgg aggtcggcac
    87781 caaaattaga aaggatacgg cttacattgg cactctgaag agcaaaccca atctggatac
    87841 caagaaaacc aaagctcata ttccatattt gccaaaagct gagatgcggt ttttgtttca
    87901 tcatttatta gtttagcgtt atatttagta gttcaaaatg tacctcaaaa ttatgcctat
    87961 tacttactgc ttgctaagca aattgaccaa tatggagata aaaattggcc aaaaatgctt
    88021 atttttatgc cttggcagct tattgcaaac gtttgcgaaa atagttgcag aaattctcaa
    88081 cccatgatag aaatctgtct tttcgaccaa tcaaaattct cagcaccagg caattgtctt
    88141 ttccaatcgc aaaacgatga tgactggaaa aaactttatc ctgccgatgc tgaaggcaat
    88201 tgtgaacttg ccaataaagt tttgattata aaaaaagaaa acattgtagt attggttgat
    88261 tcgggatttt cacgctatga taccgcatcc ttaaatgaat acagggtaaa aaattttcaa
    88321 tatattggag atattttaaa acaggcttct ttaactactc aacatattac acacgttgtt
    88381 catactcatc ttcatctcga tcattgtggc gaaagtatcg atttttcgga gtcaggagaa
    88441 ggtataaaac gtttttcagg ggcattgtat gttgcaactg gcaatcaact caaagtagct
    88501 actgatattg atggagccgg gttcgattcg ttctatacac ctgttgttag ggctttcaac
    88561 aacaattgtt gcgatattcc tttaagtaat gacacgtttc ttttcccttg gctcgaattg
    88621 cgtattttta acgggcatac acgagggcta attgtccccg tcttgcatgg taaggaaaaa
    88681 acactggtat ttgccggcga tttgataccc actatggctc acctgactct ccaacttgct
    88741 tcgagatatg acgtaaaccc tttgcttaca ctggccgaac gtgatgattt tctggacgaa
    88801 attattcaaa acgaatacat aatagctttt cagcacgacc cttttcacga ggcctgcaaa
    88861 gttaaaagga gcaaggaaga aattattgcc atcgaatgca aatgcccttt ttatttgtag
    88921 aaaattataa aataacgccc gaacctatac catcatagac ataaaagatg tttgtggcaa
    88981 atggggggtt aatttttcat ttcctcaaat aagggtatat tttttttgag ttgttaagaa
    89041 aacaattaac agtaacgtta ccattgttca aaacacttaa atcttaaatt atgaaaaatt
    89101 ttttctccat tttaattgca atgtttattg tatctaatac aatacaagct gcacaaaaaa
    89161 tagacacagt gttcaccgca agtggcaaaa agcacaagct ttattactct atacccgaca
    89221 actacgatgc ttcaaagcaa tatccaatgg ttgtagggct tcattattgc ggtggttctg
    89281 gaattcaata ccgcaatgcc ctgactcttt tgagcgacag cctcaactat atagtggttt
    89341 gcccggacaa ttcgtccaac caaatcccag acgctaacat tgacattatc acggctgctg
    89401 cagatacggc aaggtcagca ttcaacatag acgcaggctc tatgtacctt accggcatgt
    89461 catgcaatgg cgaagttacc ttcagacaag gaatgaataa gttttaccca tttcgtggca
    89521 tctttccgtg ggtaccttgg atttcagaca acaatttcag cgcttacaaa ttagattcag
    89581 atatgccaat tgtattggct gtaggaacct atgattccaa tttcaaaact ttaatgagtt
    89641 tatatgatag cttaaaagca aatgctgcaa acgtaaatct ggtattagtt cccggagttg
    89701 ggcacacttt gagctttaat gaatttgggg caacaatgat ccactgtata aaatacctaa
    89761 attctgccaa aaatatagaa atagctgcag ctaaaccaat atcgatgata aatactgacg
    89821 aagaaaaaca ggttgaaata cccattactg cgtccaattc gaaaaatatt aaagtaaaac
    89881 aagtcttatc agtaaacggg gtttttgaat caacaaccac ttatgacaaa acgagcaaca
    89941 aagcgatagt aaaaatcaag cctgttgcag gcaaatcggg tgttggaaaa attgtaatag
    90001 aagcttctga agctggagac tctgcaatag cccaaacagt tatcaatgtc agagttgata
    90061 aaaatgtcag tgtaaaaaat gaggaaatga aggctcttaa aacaacctta gcccccaatc
    90121 ctgcaaaagg acaagtagtt ctttcaactc cctcaacagc caatagtttt gaattgcttg
    90181 atatttccgg gaaaatatta attcaggtaa aagcatctgg ctcacagaca atgctcgaca
    90241 tttcaaaata ccaggcagga gtttattttg taaaaacaaa ctattccaac accagtacgc
    90301 tcgaaaaact ttgtatccaa taaaatattg gtttatttaa aaatttaaaa cattcttaaa
    90361 ctatacagtt ccgaagcttc aatgtcttcg gaactttttt tataaaaaaa gcctgagcat
    90421 aaactcaagc ttttttgtgg agaataaggg aatcgaaccc ttgacctttt gcatgccatg
    90481 caaacgctct agccaactga gctaatcccc cagtatgttt tacaaactct ttagttagag
    90541 ttttcatttt tcatcaactt actggcttcc ccaatgctat cgggtcatta tagccactga
    90601 actaatccct ttaggatttt ccggctgcaa atatcgaaca ttttttttaa ttaaactgaa
    90661 ttatttgtta aaacaagatg aaaaactttt gttttaatgc ttggtatagt ggtttgaaat
    90721 aaatgctaaa ttgttgattt tcatttttta cttcattgat tatttcaatg cttattagct
    90781 tgaaccgttc gtctatggct tttttagctt taatgcgaac ggttcaagcc tcttgttata
    90841 ttggctttta aacaaaaata acctgtacgc cttctccggc acatttttca taaaactgtc
    90901 caaccgtgat gatgtcgctg acttcttcag taaaatcttc ctttttcagc ttaaacatat
    90961 cggcagccag tttgcaggca aacacttcgc cacctccggc tgtaatcatt tccaggaatt
    91021 ctttcacagg cgggatgtcg agtttgtcca tctggctgcg catcatggca gatactcctg
    91081 cttcaactcc cggcaatgct cctaaaagcg tcgggaatgg taatcctccg ggcatacgca
    91141 tggccggatt gcctaccgtg gcggtatgca ggctgttcat ggtctttttg gtgatggcat
    91201 caaggccaaa gaaggtgaag aacattttag cttcaatgcc ttccattaca gctccatttg
    91261 ccagtaccaa tgctgcatat acatcttcga ggtttccttt ggcgcaaatg ataagcacct
    91321 tcttcaatgg attttgtacg ttgctcatgg ttattgtttt tatggttata cacaactcaa
    91381 aggtttcatt attcctgcaa tttttgaaga aaccttaagc gggcccccgg gaaaaagctg
    91441 atagaattct ttgatatcta ctatcccgga agaacctacg cggcggatag tgagtgcttc
    91501 accggccatt actcgttggc ggagaaattc caacacagca aagtgcttgt cggtaagcgt
    91561 aatgttgtat tcttttgcaa tttcaactgc tatatcgcgg ttccattgcg aagggtctgt
    91621 catgtaccct tcatcattta cctttacggt aactcctgct attacttttt cagccataat
    91681 tttatttttt aaaggtttat attatttcat tttaagtgag ttttcattca tcctcacatt
    91741 ttcaaatttg ctaattcact catttgctaa ttttcttccc aaccatcgac atcttgttgc
    91801 taatgggcaa ataccgtcct ttaatcagga tattccagta aatccattca aaagcaagtt
    91861 ttccccagtg gtttagcctt gtttctttaa gcaaagacat agggcctatt accggaagcg
    91921 ggaatgttcc ggccaatggc tcctgatcgt agttaaagtc aatcagtgca gccttcccaa
    91981 agcctgtttc gatgaagcaa ttggcgtgtc catcaaattt agcggttggc tctttacctt
    92041 caatcatatc caaaaggttt tccagaagaa catcggctgc aaaatgggct actgcccctg
    92101 cttttgatgt tggtattgct gcggcatcgc caaggacaaa tatctctgga tattgttgca
    92161 tttgcagagt ttccttattc acgggtatat agttgaggtc gtcgccaata ccactgtttt
    92221 ggatgaactc agcgccttta ttaagtggaa cagtaaccag taagtcaaat ggcacttcct
    92281 tctcatcata cgaaacaagc tttttgcctt cattgtcaac gtgctcgata taaaaatctt
    92341 gaataatgtt gatgttcttt gaagataata attcactaag tttagcggtg gctacaggtt
    92401 tggtaaaagc tcccggaagc ggggttacat agctgatttc aactttatcg cggatgcctt
    92461 tttgggtaaa gaatgcatcg gccaggaata caaattctaa tggcgcaaca gggcatttga
    92521 taggcatatc cacgatggcc atcaccattc gcccgccttt ccaggtttct aatttctcgg
    92581 caagggctac agcgccttca taagtataaa attcaaaggc ttctttttgc caaagcggcc
    92641 cattaagtcc cggggtgtca tcaacagatg gctttgtgcc ggttgctaca atcaggtagt
    92701 cgtaggtgag ttcctgtcca ctttttagca agacagcttt tctggctggt tcaagtttgg
    92761 aaactttatc tacaataaag tttacacctt ttgggatgta gtccttttta ggttttacaa
    92821 acttgtcttt aagcttctta ttaaacggga taaacaggaa cccgggctga tagtaatgct
    92881 tttcgtccgg atcgactatg gttatttccc attcatgaac aggcaaagtt ttatacagct
    92941 tgtttgctat catggtgcct gctgtcccgc ctccaatgat gactattttt cccatatttt
    93001 atttttttat tgtatcttgt aacgtctttt atcaaaacaa catttgaatt gttatttttt
    93061 taacaaagaa acgattgcca tggggcgaat gtttatcaga aaggcatgat aaatatcagg
    93121 acatgatttt tatcatattg atgaaaagac atttacaagg ttttgggcaa aacaaatatg
    93181 ggcgaatatc aatgtagttg caacggttgt gagttacgtt ccctcttttt cgagaatttg
    93241 gaaaaagggg catacgaaag tatgtgcgcg aaaaagcagc agcagttgta tcctaagggt
    93301 agcctgattt atagacaagg cgaaaagatt agtcatttct catatctcaa atcaggattg
    93361 gtaaagcttg tgcggaatat tgatgataaa aatgagcaaa tcatctgttt tgcccgtcct
    93421 ttagactttg tgagcttgtt gagtattttt tccgagacct gtcatcaata ttcggttata
    93481 gcacttgaag atagcgagat ttgcagcctc tatctggatg aagtaaaaga aatggcgatg
    93541 cagaacgggc gttttgccct aagtttggcc gaaaaaataa accgcgccag tgacaatatc
    93601 atccttacca tgttaaacat caggcagcgc aggct
//
LOCUS       DAOSPF010000003        87095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_268_length_87095_cov_7.525230, whole genome shotgun sequence.
ACCESSION   DAOSPF010000003 DAOSPF010000000
VERSION     DAOSPF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 87095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87095
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_268_length_87095_cov_7.525230"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PK252_00980"
     CDS             complement(<1..611)
                     /locus_tag="PK252_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT13307.1"
                     /translation="MKHIKYFIQIILVAALFASCSESFLDTTQEGVQSEETFYNSLTN
                     IGQAVTGTYGSLNIQPAGIHNLDMMYLVFGSIASDEAEAGGEQGGNDFLDIQHADLGT
                     IQPSEPKSLSDAFWAYNYKSILRANSTLAGINKYKSSNSNIPADSLKLLNQYEGELYF
                     ILAFVHFKMVQVYGGIPIVDHPLSSSEYGIKRNTIAECLHAIQG"
     gene            complement(650..3943)
                     /locus_tag="PK252_00985"
     CDS             complement(650..3943)
                     /locus_tag="PK252_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13308.1"
                     /translation="MMQKSFIKLFWLRLRYLLLAVTLLWAYPVFGQNKSVSGVVKDAT
                     GTGIPGVNVIVKGSNVAVVTDVDGKFTIKCSLGDVLTFTFIGYKPSEVTVGESADINV
                     VMQEDVKELNEVVVIGYGVQKKSDLTGAVASVSSKDLKNMPTARVDEALQGKAAGVQI
                     YQNSGQPGAEPKIRVRGLATINGGNPLVIVDGVPGGSLGNINPADIESMEILKDAASA
                     AIYGSAGGNGVILITTKRGQTDRFQANLDMFWGLQEPWSKDNVKVCDAQQYAEIYNRS
                     KANYFPYDNVNKVYMDHSDSTKALTNTNWVDKIFRSQALMQNYNLSVNKGGKKYNVYS
                     SIGYNGEDGTVLRTYNKKYTFRLNSDYQVMKRLKVGESFSFSQRENSTQNEMNEYNSP
                     LSTAIQMLPMVPVYSTDGTGNYAYKGSDLSSGVKNPLAQIEYNNNVNNSRSFNGDVFL
                     KLNVIEGLNFETHYSLNSSNSEYKNFTPVHTIGDTAAANPTQSVKINSYNTGTYENTG
                     WNYQGFLTYDLSLSDMHKFNAVAGFESGEYIVKIKDKSASGVNLDVADWKNFDLNDTA
                     FKNIHDPIKKTIRGYAFFGRLSYDFANIVLLQANIRRDYSSKFGANNRAGTFPAFSAG
                     IKLSEIEAIKDLNIFDLLKVRYGYGETGNSDIQPYLYNSTIGILPINGYVFNNVKNSG
                     AALITAGNADLHWETVVSQNIGLDFTVLKNKVSVSVDYFTRRNKDMLLRKSVPYTVGY
                     AILGGGNSQELGDGSIDTRPLVNYGTLDNSGYELTVGYKEQIGDLLVNLSANITHTKT
                     VIDDIGDMLQAGTGRGVSNVCRTQNGHAVSEFYGYQVDGIYKEADFVWYKTALKRWKQ
                     VAPDATGAVVVEGFDVNGNPVTYNTKSSTAAPGQFKYKDINNDGVINSEDITNIGDPN
                     PKFTYGFNLNLEYKGFDLSAFFQGSYGNKIFNLLKVNSYTINNGSLNLSPDLLNGYIP
                     SKWNTSPNLAQPERLSEPQNENTGIAAIDAVLLPSSFYVEDGSYLRLKNIQLGYTLPK
                     SLTQKIKIEKLRVYVGAKNLLTFTKYTGFDPEVGENTTVSNSILERGFDRGTYPQSKM
                     YLFGMNITF"
     gene            4432..6921
                     /locus_tag="PK252_00990"
     CDS             4432..6921
                     /locus_tag="PK252_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT13309.1"
                     /translation="MIYPSNFEQKIGFDRLRARLKTLCISSLGKDYVDNVHFIKDFEL
                     LQHLLSVCNEMIVILKTEENFPAENFIDCTSYLNRLKIEGTFIEPEEMFKLSLSLTTI
                     KKLTHFFKEKGEGKYPILFSKSAEVKVFSFVQERIDQIVARNGSVKDSASPALHKIRK
                     EMAALETEISRRMARIMEHARQQGWAEDGASPAVRDGRLVIPIDATHKRKLKGFIYDE
                     SATGKTVYIEPAELVELNNDLKEAEHAERREIIKILIGFANDIRPYIDELLIGYDFIG
                     LIDFNRAKALFANETQSVIPPLNNRPVLEWYNAVHPLLYYHLKEEGKKVVPLNIRLNE
                     MHRILVISGPNAGGKSVCLQTAALLQYMLQCGLPIAVKETSETGIFDQIFIDIGDEQS
                     IENDLSTYSSHLLNMKHFVKNANNKTLFLIDEFGAGTEPMLGGAIAESILEQLNTIGA
                     YGIVTTHYTNLKHYASQAQGIVNGAMLYDTQHLQPLFQLVIGEPGSSFAFEIAKKIGM
                     PEVILKAATEKVGEKHIDYDKNLKEIIRDKYYWQQKRNQVKDTAKKLDEITSRYTAEL
                     EDIKKLRKETLKKAQEEAQAILAGVNKQIEHTIKEIREKQAEKEITKQLRQNLEKKKL
                     EVDLALNIEDERINKKIEQLRRREENKKRRDAEKSNIPEIKKPEKPQKAEFETGDKVK
                     IFGQDNIGEVISVNGKNIMVGFGNMITTLDEKRLVKISHNEFKKIQKSNQSHTPFASR
                     SFDIIEKKKNFKMQLDIRGMRADEAMQKVADYIDEAIIVDVSEVRILHGKGNGILRQL
                     VREYLQTVELVKSFGDEHVDMGGAGITLVRF"
     gene            7052..7972
                     /gene="metA"
                     /locus_tag="PK252_00995"
     CDS             7052..7972
                     /gene="metA"
                     /locus_tag="PK252_00995"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOT13310.1"
                     /translation="MPINVPDKLPAIDILKEENIFIIDETRAAEQDIRPLKIGLLNLM
                     PIKQTTETQIIRMLSNTPLQVELELISLESHVSKNTSQEYLETFYKKFNEIGQSKFDG
                     FIITGAPIEHLEYEQVRYWPEIQKIMDWTQKHVTSTLFICWAAQAGLYHFYNIPKYML
                     PAKMFGVFEHHVINPSLPLVRGFDDVFMAPHSRHTEVREEDIRKVKELEIVAKSNEAG
                     VHIAMAQEGKQIFVTGHFEYDPHTLRTEYFRDVDKGLTIDLPKNYFKDDDPNNHPIVT
                     WRSHANLFFSNWLNYYVYQQTPYNIDKIGS"
     gene            complement(8049..8693)
                     /gene="nth"
                     /locus_tag="PK252_01000"
     CDS             complement(8049..8693)
                     /gene="nth"
                     /locus_tag="PK252_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT13311.1"
                     /translation="MKREELFEKVQQYFQKTMPVAEPELHFRNEYELIVAVILSAQCT
                     DKRVNMTTPAFFEKYPTPLALSKANVDDIYQLIKSISYPNNKAKNLLAMAQMLVTEFG
                     GKLPTDTQQMQRLPGVGRKTAHVLGAVLFDKPVLAVDTHVFRVAARIGLTVNAKNPLE
                     CEKQLTKYLPADILPAAHHWLILHGRYVCLARSPKCKECGLTEVCKHFKTMINY"
     gene            8797..10851
                     /locus_tag="PK252_01005"
     CDS             8797..10851
                     /locus_tag="PK252_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT13312.1"
                     /translation="MKNIKFIVLSMISAGILSSCSHQPSSCSTNSADSFAWKTEQFAD
                     IKILRYQVPDFEKLNLNQKILVYYLSKAANAGRDIIWDQNCKYNLLIRSTLENIYKTY
                     SGDRNSANYKKFEVYLKRIWFSNGIHHHYSTDKIMPEFDKAYFAELVKNSDQKGFKLP
                     EGKNLEQTLTMIEPVMFNANVMAKKVWLDPSKDLIANSATHFYDGITEKEAENFYSSL
                     KDPKDETPVSYGLNSQLTKVNGKITEKVWKVGGMYSPAIEKICYYLNQAIPYAENEKQ
                     KVTIEKLISYYKTGNLKIWDDYNIEWVTDTISTVDFINGFIEVYGDPIGLKGSWEAMV
                     NFKNLEATKRTEILSANAQWFEDNSPIDPRFRKAKVKGVSAKVITAVQLGGDTHPSTA
                     IGINLPNADWIRKEHGSKSVTLENITYAYNQVSMGSGFYEEFIENPADIELIKKYKAL
                     SDNLHTDLHECLGHGSGQLLPGVAGDALKNYQSTLEEARADLFALYYMMDQKLIDLGI
                     LPSLDAAKAEYLSQMLNGLMTQLVRIEPGKTIEEAHMRNRQLIAKWCLEKGKADNVLE
                     LYKHNGKTYLRVNDYLKLRTLFGKLLAEIQRIKSEGDYKAGRDLVENYGVQVDKAIHT
                     EALERYKKLNLAPYSGFINPKLSLVKQGEKIIDVKIEYPDNFAKQMLEYSNEFSTLPV
                     LN"
     gene            11048..11551
                     /locus_tag="PK252_01010"
     CDS             11048..11551
                     /locus_tag="PK252_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13313.1"
                     /translation="MILRKKIRMRKRSKKICNFDTAQNIGVIFKTDSQSDFELVKRFL
                     QFLSDHNNKVFALCFVDSKKVPDFYLLRKGFNFFSRAELSFFFIPKSQTIINFSEQPF
                     DMLIDLSIDESFPVHYISAISNAKLKLGKQKKNSDHIDIMIDVRKNNSVSQLIEGIKQ
                     YVPVLCQ"
     gene            11641..12531
                     /gene="dapA"
                     /locus_tag="PK252_01015"
     CDS             11641..12531
                     /gene="dapA"
                     /locus_tag="PK252_01015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT13314.1"
                     /translation="MNTANSLFKGTGVAIVTPFRKDDSIDFTALSNLVNYQIENKADY
                     IVVLGTTGESVTLSNDEKHAVINTVIEAANKRVPLMVGIGGNNTQEIVTKIKSFDFTG
                     IDGVLSVSPYYNKPTQTGIYEHYKAIANISPIPIVLYNVPGRTGMNVTAETTLRLAND
                     FKNIIAVKEASGNMQQVMDILKNKPENFSVISGDDALAFPLACLGCDGVISVTANVAT
                     KEVSNMIRFALEQKYDQARKLHYQLLDLTTMFFTEGSPAGVKAALDVKKLTSAHVRLP
                     LVPVSKSLYDKIAEKMKEFK"
     gene            complement(12610..14046)
                     /locus_tag="PK252_01020"
     CDS             complement(12610..14046)
                     /locus_tag="PK252_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOT13315.1"
                     /translation="MRTESDFIGTLEIPDNVLYGIHSLRAKTNFPDDTPFSKEWYCSV
                     GLVKLAVYHTYKNYKKAVSEKYSDKALPFALMSDEVIEALIKAAEEVEAGMHFEYFIV
                     PALQGGAGTSINMNVNEIIANRALQLLGSAPGNYSLVDPFEHANIYQSTNDVIPTSLK
                     VCVIRLLEVLEKSINELRSKIEFIEKQAHQHLRIGYTQMQEAVPTSFGRLFSTYNEAL
                     SRDWWRVSKCYERIKTVNLGGGAIGTALAVPRYFVFESARNLSALSGLPIFRSENMPD
                     ATSNLDAFVEVSATLKAHAVNIEKMTNDLRLLASDIAGREIKIPQKQAGSSIMPAKVN
                     PVIVEYAISGAHQVYANDALLTNLSALGCLELNAYIPSIGHAMINSIKTLISINKTLA
                     DNLFTGLELSTEEAKKRMLYSPSISTALVPVIGYKKATELALYMKQHHINIIEANKAL
                     MFVSEGQMQRALDLANLLKEGYSLEDIF"
     gene            complement(14071..14781)
                     /gene="rsmI"
                     /locus_tag="PK252_01025"
     CDS             complement(14071..14781)
                     /gene="rsmI"
                     /locus_tag="PK252_01025"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT13316.1"
                     /translation="MKTNLYIVPTPIGNLDDITLRAINVLREVDVVVAEDTRQSSVLL
                     KHLGISKPMVPFHKFNEHKTLSNLINRMQGGEVFALVSDAGTPSISDPGFLLVREALA
                     ADLHVECLPGATALIPALVVSGLPSDRFCFEGFLPQKKGRQKRLAMLSQETRTMIFYE
                     SPMRIVKALEQFVQFFGAQRKVTVSREISKLHEETLRGTLEEVIAELKSRPSVKGEIV
                     VCVAGLDTKGSHTDEDEE"
     gene            complement(14785..15504)
                     /locus_tag="PK252_01030"
     CDS             complement(14785..15504)
                     /locus_tag="PK252_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13317.1"
                     /translation="MDKSAVDKLKKYHSKIAEMLNSIGEKNVISSIEKDIVLKNLREM
                     YEAVLEIKIDKAVEGPVKEAVKKVEPVKIKQGLEDVIVKEPKMQKNEVVEQPKEDELI
                     VETEPLAVKEKIVEQLQETHEKQQHHKQILAEKLAKPEPLLHDVVSQKVNVTDLSTKF
                     QQKEIASLTQSIQINDRFLFIKELFRDDAKLYAESLQKIEEMKSAEEAVLFLKAEINI
                     DLEKDAAQKLLQLINRKFANA"
     gene            15664..18114
                     /locus_tag="PK252_01035"
     CDS             15664..18114
                     /locus_tag="PK252_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOT13318.1"
                     /translation="MDYSLKQIAEITGGKLIGDGSVMINQLITDSRQPVAACGSLFFA
                     IKGPRNDGHQYIHDLIAKRNVTNFIVEKEISVEQKINQIIVDNTVNALQKLAAYHRSC
                     FNCPVIGITGSNGKTIVKEWLYQLLQSKYNIVRSPKSYNSQTGVPLSVWNMNEKTELA
                     IFEAGISKQGEMSRLQAIIKPEIGIFTNIGEAHQENFYSIQRKIREKLNLFRDTKVLL
                     YCLDHEAIAEEIEDWANNNQQLICWSAKQKADLQITRQISQNNSTSLTGTYKDIEISI
                     NIPFTDNASIENAITCWLLVLHLGIDKSIYQPEFAALSPVALRLEQKKGINGCSIIND
                     SYNSDLYSLGIALDFLSHQQQLQKTLILSDILQSGRLDSDLYQHVANLIEIKQVQRLI
                     GIGPSISSQASLFKVKKEFYTSTEEFISHFQPSRFQNEAILLKGARNFEFERISSFLE
                     EKTHRTTLEINMTAMVHNLNYFKSKLHAGTKLIAMVKAFSYGSGSYEVANMLEYQKVD
                     YLAVAFADEGVALRQSGISMPIIVMNPEASAFRLMIDYRLEPEIYSFKELRQFADMLK
                     AYRIDNYPVHLKVDTGMHRLGFMPEEIDSLCNEIKKNKGIKVESVFSHLAGADEEQHD
                     NYTLEQIELFQKICSKIQNCVEYPVMRHILNSSGIDRFSEYQFEMVRLGIGLYGVIPS
                     NQEQLQWVSTLKTSILQIKEVETGKTIGYGRKGKALKQTKIAVIPIGYADGYSRILSN
                     GVGRVFVNGSYAPVIGNICMDMCMIDITGIDANEGDEVEIFGKHVTILELADKMHTIP
                     YEVLTSIAPRVKRVYVQE"
     gene            complement(18277..19125)
                     /locus_tag="PK252_01040"
     CDS             complement(18277..19125)
                     /locus_tag="PK252_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT13319.1"
                     /translation="MKSKNLKAKLFVFACVVLLGMGLNAQNPNFYIYICFGQSNMEGQ
                     GAIEAQDSIVDSRFKVFQALDCPNLNRTKATWYNAIPPSCQCHSKLSPADYFGRTMVA
                     NLPESITVGIINVAVGGCDIRLFDKDIYQDYDSTYKESWFVNKVKDYQYNPYKYLIDL
                     AKLAKKDGVIKGILLHQGETNTGDAQWPTYVKKIYNDMLADLSLKAESVPLLAGEVVS
                     AEGSCCSSMNAIINKLPETIPTAHVISSAGCPSQDNAHFNAEGYRILGKRYGVKMLSI
                     MGIKAK"
     gene            complement(19169..20755)
                     /locus_tag="PK252_01045"
     CDS             complement(19169..20755)
                     /locus_tag="PK252_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT13320.1"
                     /translation="MSYYQYVVKDVLRKNIYFFSLVVLLAGLPLSKALVSIGEGLLVF
                     SWFVAPGFKERVKALGQRKSIWIFAGSTLFVLAGLFYAENIQRNIFLITNIKISIVLF
                     PIIIGSFEKLSWQQLKVLLWVYTLANIAATFFSLLIFFHVIEYKIANVRQIAVFVESV
                     RFALLIVLDILFLLYAIYHSQNRKFQVLMIISIVWLVVFLGILQSLTGILALALTLLV
                     VLLVRYRKISVYIRVAFVVLGLLSIATGAYLFKSNLNEYRAIHIENTAKLDSLTLNGN
                     KYEHSVNDTIRVNGYLRNIYICEPELRIQWAKRSQVAFDSLDNAGWVPVKVTLVSYLT
                     SKGLRKDSAGMSQLTDRDVRNIESGYYNYKLAEVGVNARIYDLIREFDEWRRTGIPAG
                     SVTIRINGLKNGWKVFSEHIWFGTGYGDINDDIQAQFDKDSPDLGEDKKQNPHNQLLT
                     IMIGSGIVGLVVFLLSFVLPGILERKYSSYLFLVSFCVVLFSFFTDDTLERMIGCVIM
                     ALFFSLFLYAVPKNINLIKK"
     gene            20901..23111
                     /locus_tag="PK252_01050"
     CDS             20901..23111
                     /locus_tag="PK252_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT13321.1"
                     /translation="MKQSQNILLSRIDAIGDVVLSLPVAGILKNLFPDCTIFFLGQTY
                     TKDVIKTCQYIDNFIDWNQIKDLNPKTQIDFFRKLNIDVAIHIYPNFFISWILKSAGI
                     PIRIGTSRRWYHQLFCNKKISFSRRSSNLHEVMLNLKLLIPFDLGKTINYETLSIING
                     IIPPTIPDELNSLLDKNRFNLIIHPKSKGHGKEWGIENYAKLLGLLDNSRFNFFVTGT
                     LDEKKEIQQEILNKYPQAIDLVGKLSLDELIGFISRADGLVASGTGPLHLAAATGIHA
                     IGLFPPIRPVFSRRWAPVGVNAKAFEAKKICVKCKKNAKCDCLRLFKPEIVAEYLNTL
                     HKPSQKKQLEANIFKPSALDWEQFRIQQAQKFENFVSDKKTKVFTSTNQIFKKNRIIV
                     SVDKINKPNCGLGRVAIDFSKALINEKKENCYFSFILTGNNSYDYFRDQDIKRFKFIH
                     RFFKADFGEYDVAHYLHQTPSFKIKGAKKTILTIHDLNFLYTKSNIKANRYLKHIQRN
                     VDKADAIVFISNFTKEICFQHLQISPEKITRVIYNGVNLPKTIPIKPPFINGTTFIFS
                     IGQFLAKKNFHTLIPFIKLFPSNIQLVIAGEKDTSYGNYVRKLISDYNLQNQVILPGS
                     VSEAEKLYLYQNCEAFVFPSLAEGFGLPVIEAMRAEKPVFCSDKTSLKEIGNGHVFFW
                     ESFDPQQMFMTFQNGMKSFDEAKKKSAYEYSMKFTWEENARQYLSLYHLFTENE"
     gene            23104..24132
                     /locus_tag="PK252_01055"
     CDS             23104..24132
                     /locus_tag="PK252_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13322.1"
                     /translation="MNKIFIYCPGNIISGGTNSLHILCKALEKNGFEAGMYYINPVEK
                     IINSDIVRSFQTTSFGPPKDLEQNIIIVPETMVGYLKQFNNAQKIVYWLSSVYFFKNP
                     PFKFPFNIKIFRKPIYLDWYTYQSQSLSSRLRVRLLKWAKKDSQIWQNSYHHITNSFF
                     ISDFLSFLGIPQTFVLHNPVRDEFYQPLANTPKQKLILVGPKTPKNILYIIKKRFPGF
                     QVLQAKKIPPEEMFLLYQKSILFIELGHSNRDRCIREAALRGCIILVSNSGGNKNQHD
                     TPIADYYKINTSKKYNNLLLNKIDEIIKNYDKHLANFEHFRTFLLEEKKQFEKRALLI
                     FEQITSKN"
     gene            complement(24129..25538)
                     /locus_tag="PK252_01060"
     CDS             complement(24129..25538)
                     /locus_tag="PK252_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOT13323.1"
                     /translation="MKNIFFFLLFLSQSIIVFGQMIEKPFVYGETGGFVSNGENAPFW
                     LYSNQFGVFNNNKNCLYARVLMGTEENREKKFEVNYGMDLYGIRDKSNNLQFNQLYAT
                     FRAFCIIFKGGLYRQVYGNQTSELSSGSVLFSSNARPYPKVSLGIDKYTFVPFTKGYV
                     EIKGEVTHGWAENENRWVRDSYIHYKYVNIRFGGKKPLHFNYGYHHALQWGGVSSTLG
                     RQPSSLKDFVYLFFAKNPPGANVVQSESFNQVGNHMGSRQYGLDYDFKNKTVGLYYQT
                     IFEDKSGSKFISPQGDGLYGLTFKNNDETAHLKEVVLEFTKTTYQSGPIELVWVTDVP
                     IPFWNPKAGNDNYFNNYIYQSWTYKGNTIGTPFITSPAINEKDSNIVTNNRVLAFYFA
                     GLYEYNHLQCELKYSYSINKGTYSVPIGEKGQHSMMFKVGRDIPSLKDLHVQLCVGWD
                     KGAFLGNSFALGVCAKKKF"
     gene            25868..27733
                     /locus_tag="PK252_01065"
     CDS             25868..27733
                     /locus_tag="PK252_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT13324.1"
                     /translation="MKKSLLLLALAGIYCIVSGQYSFIQDFKNGTQPETGWVIHVGGT
                     YNSEPNSGKAIPSLMFSANNQYIETPSFKGQHSLSFWMKGNKTDSASTMQVLIFCNNN
                     WYLAHKISQISQTGANYSFMLPDTAEKLKFEYKKSAGNVALDDIVLFAKNLFPEITYI
                     RLTENNDVAALINIKTNNIGKITYLVTSKDEPSPNAAQIADTSLYSGKILDYGKTDIT
                     KPDSTSIVLQCLPSNSNLKAFFIVADLKDSFSIEQQPVKLGINTPERNRYPFFKGIYK
                     GDASAEKAIQIFNPTKETINLTNYRILVATNGKGWGFDYYFENGKSIKPGSTYLIVNA
                     GAEFIDIDKADEITTSVLCKFSGNDALALQKKFDNSNAWYIIDIFGNPYSSANFNIAG
                     KKSAANKYNAIRKDSVDTGNVNWSVSAGNTEGDSEWELIALSDFGLLPIVLPPYMPKL
                     SFIDISIDGIAAQSIDSINYTITFSTNGTVNDLKLNYRLDSAALIEPLPDSIRDYSQP
                     VIYSIFSKESEDTVKWKIIVNGNKNIITNITNEKPFIYYNQQNKTIVIKQTSNENFSY
                     AIIYNSSGQVIKKAKLNFENNFINIPKLPSGLYVIKLLKNNAKYSKSTTINIFRR"
     gene            27896..28024
                     /locus_tag="PK252_01070"
     CDS             27896..28024
                     /locus_tag="PK252_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13325.1"
                     /translation="MKKIIYLGIIVVIALCVASCKSSKKCPAYSFQKANTEQVVKA"
     gene            complement(28083..29648)
                     /locus_tag="PK252_01075"
     CDS             complement(28083..29648)
                     /locus_tag="PK252_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT13326.1"
                     /translation="MQQQVSLKLNAQKTDDSFNCLCPVVAEQLQINIERIKHIKIEKK
                     SLDARRRPILVNYTLLVFIDEQPPKEDIQPKIYKKVDHAKPIIIVGTGPAGLFAALKL
                     IEHGFRPLIFERGKEVSERKKDIALISRNEEVNAESNYCFGEGGAGTFSDGKLYTRSS
                     KRGNIKDVLDVFVRHGAPESIMYEAHPHLGTDKLPEIIKNIRQTIIAAGGEFYFERKI
                     TQLLIEDEEIKGVQLHNGDKVEAQAVVLATGHSARDVYQMLHEQHIALEAKPFAVGVR
                     VEHLQELIDNIQYHGLKRGENLPAASYSLVCQANGRGVFSFCMCPGGFIVPSATTNTQ
                     VVVNGMSPSKRNSPYANSGIVTEVRLEDLKEFAKYGPLAGLYFQEQLENMAWQNGGRQ
                     QTAPAQRLTDFIKGKISGSLPQISYFPGCVSSPVHMWLPGFVGSSLKEGFKRFDNLMH
                     GFVTAEAVVLGVESRTSSSVRIPRNQENMQHVQIKGLFPCGEGAGYAGGITSSAIDGQ
                     NVANAVVNFLKQA"
     gene            complement(29652..30386)
                     /locus_tag="PK252_01080"
     CDS             complement(29652..30386)
                     /locus_tag="PK252_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOT13327.1"
                     /translation="MQAYKKYILFFILVVCCQILGWGQKSKYKHNPEYDDKPLHFGYT
                     MGLNVMDFTFGRNYNGLNENYYADQLGLGLGFQVGMISDLRLGEYFNLRCLPSFNFGQ
                     RTLQYFSGKSVYKVDTKMQEVKLESSMLDLPILIKYKAMRLNNTRPYFIAGGSVRYDL
                     ASKSKFDDKHPYVLLKPLDFYLEMGFGVDFYLEYFKFSTEIKLSLGLTDILNHKTNEN
                     IKENAVYVNSIRKLNSNILMISFHFE"
     gene            complement(30402..31124)
                     /gene="ubiE"
                     /locus_tag="PK252_01085"
     CDS             complement(30402..31124)
                     /gene="ubiE"
                     /locus_tag="PK252_01085"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOT13328.1"
                     /translation="MVIPYKDTQGSKKSQVRQMFDNIAFRYDFLNHFLSMGIDNFWRR
                     KAISKIKHIKSGIVLDVATGTGDLAITTAKRTKLDIIGIDLSENMLSIGREKIEKKGF
                     AQRIKLQQGDSEGIQFPDAHFDAITVAFGVRNFENLDKGLSEMYRVLKPGGKVVVLEF
                     SKPKYFPVKQIYYFYFRKILPFIGRFISKDRAAYTYLPESVESFPDGKAFLEHLTKAG
                     FVQNTCKSMTFGIVNLYTGDKI"
     gene            complement(31135..32010)
                     /locus_tag="PK252_01090"
     CDS             complement(31135..32010)
                     /locus_tag="PK252_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT13329.1"
                     /translation="MQLSMTGFGKATGVFANKNVTIEIRSVNSKQLDINLKLPLFYRE
                     KEAEIRNIVGRAIERGKIDLSVTVEQSVATSNYAINADVVKDYYRQLKDLAEELGVTV
                     GEQLYTSVIRLPEVLKNEAQGLIEEEWVSLTKLINEALADFIQFRRQEGAALEHDVKQ
                     RVNTIDEAIKKIEQFEKQRIDKIKQRLSKNLNEFIPAASIDANRFEQEIIYFLEKIDI
                     TEEKVRLHNHCQFFIQTITNESAAGRKLGFIAQEMGREINTIGSKANDTDIQRIVVMM
                     KDELEKIKEQLLNIL"
     gene            complement(32099..32800)
                     /locus_tag="PK252_01095"
     CDS             complement(32099..32800)
                     /locus_tag="PK252_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT13330.1"
                     /translation="MESIISIKDLRKDYYVGKIVVPALQGVNIEINKNEYVAIMGPSG
                     SGKSTLMNILGCLDVPTSGDYILNGTSVSKLNDNELATIRNREIGFVFQTFNLLARYN
                     ALENVMLPLVYAGVEKEKRIEKAKQIIDMVGLTERMHHKPNELSGGQRQRVAVARALI
                     NNPAIILADEPTGNLDTKTSIDIMRLFNDIHQRGNTIILVTHEEDIALHAHRIVRLRD
                     GLVESDYRNEKVRLD"
     gene            complement(32825..33118)
                     /locus_tag="PK252_01100"
     CDS             complement(32825..33118)
                     /locus_tag="PK252_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOT13331.1"
                     /translation="MNQQINAAIQVLPRSKDKHPYDIVDKAIEIIKTSGLKYQVCPFE
                     TVVEGTYAEVMTLVEKVHAACYAAGTEDMMCYLKIQSSSQKAVTIDDKMNKYK"
     gene            complement(33115..33867)
                     /locus_tag="PK252_01105"
     CDS             complement(33115..33867)
                     /locus_tag="PK252_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13332.1"
                     /translation="MEETGALTDPLEDLRKLLVDFKGEVNIINERIDVDLQVKYFDLS
                     QKYRNKKLSLPEGELNVELLDDESADEEIKKILLIQLAHSKDVKSFRIIEKFVSQKHP
                     VLYQWSLMALQEAQMYIQSDLLEQPQIFLCTGLGGRDRKLRYFIAFFNREGLDFTDWQ
                     QKTIKNEVELGLPVYDAELEDIQFERFLAKIILLIPIVSPIHHFMRDVINKINIFGNF
                     VDENCIVTNVRILSFDEILQVANKKEDITDKL"
     gene            complement(33941..35086)
                     /locus_tag="PK252_01110"
     CDS             complement(33941..35086)
                     /locus_tag="PK252_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT13333.1"
                     /translation="MRIILIFLKKEFAQIFRNKMMLPIIFVIPLVQLLILVNAATMEM
                     KSIRMVIVDNDLSMASRRLASHFLASKFFVIDNYMSSAIQAQQLMQDGKADVVLCINR
                     DFEKQLLNESSAKVQATFNAINGMVAGIASSYIQAIMADYQAELIQSGRYNRPVSIGV
                     AGTNVAPAQLNIQHRFWYNPELNYKCFMVPGVLVVLVTLVGMFLAGLNLVREKEIGTI
                     EQINVTPVKKYQFIAGKLIPFWILALIELGVGLTIGKLIFDIPIEGSLFLLFAIAAIY
                     LVIVLSIGLFVATITETQQQAMFIMFFVMIICIMMSGIFTSVETMPNWAQMVNHLNPA
                     YYFMRVIRMILLKGSGFKDLLFEISCMGIYAVAMLTLAVRKYKKAVA"
     gene            complement(35098..36201)
                     /locus_tag="PK252_01115"
     CDS             complement(35098..36201)
                     /locus_tag="PK252_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT13334.1"
                     /translation="MKTYLSLVKKEFHHILRDKRTMLILFGIPVALMLLFGFVITNEI
                     KTIGIAVLDQSNDYTTNQIVDRLESSCYFVVKQRISNYGQLESVFKSGAAKLAIVFEQ
                     QFDYNLQRNRKANVQIIADASDANFANLSVSYATAVIRGYINDKNVQKSGMLQIVPEV
                     RMAYNPELKGVYFFVPGIMALILMLISALMTSVSIVREKEYGSMDVLLVSPVRPVEIL
                     LGKVTPYILLSFINAFVIVVLGWSVFGLPVAGSLFLLFFECFLFISLALSLGILISTI
                     ADSQMVAMFVSAVGLMLPTILLSGFIFPIENMPLILQWFCNIMPPKYFIIILKHIMLM
                     GNGLKEVWKETLILLSMNLFFILLAIKKFKIRR"
     gene            complement(36232..36879)
                     /locus_tag="PK252_01120"
     CDS             complement(36232..36879)
                     /locus_tag="PK252_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT13335.1"
                     /translation="MKAVIFDMDGVLVDNSKVHDQTWQMMCQKYGRPEMAPLVRDIFG
                     GTNKVFVEKLLGINDAKRVNEIAIEKEALYREVFASSISAPDGLMNLLFELRKNNYRL
                     AVGTSAPTENLDFVLDKLDIRNFFDILVDESQVTHGKPDPEIYLKSSKLLGIEPKNCL
                     VIEDSVLGIEAAKAAGMKVVAITTSFTPDKLVKADKIINNFNELTMSDVSSILNS"
     gene            complement(36968..37729)
                     /locus_tag="PK252_01125"
     CDS             complement(36968..37729)
                     /locus_tag="PK252_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT13336.1"
                     /translation="MERFQDIDKADSTVIWVKNMTKKFGKFVANDNLTFSVEKGEIFG
                     FLGANGAGKTTAIRILCGLLSPTSGEAYVAGYDVYKQSEKIKQSIGYMSQKFSLYDDL
                     NMMENIRFFGGIYGLDKKTIEQRTNELIEKFGMQDMQKKLIKDIPLGWKQKIAFSIAV
                     LHKPKVVFLDEPTGGVDPITRRQFWDLINETAEMGITVFVTTHYMDEAEYCNRVSIMS
                     EGKIIELGKPKDLKEKHQVNSIDELFFKVARNVNY"
     gene            complement(37733..38629)
                     /locus_tag="PK252_01130"
     CDS             complement(37733..38629)
                     /locus_tag="PK252_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT13337.1"
                     /translation="MVRLNNLCKTYGTTKAIEDISFEVNENEIFGLIGPDGAGKTTIM
                     QIVATLLVPDSGIAEVCGMEVNANFKALRKHIGYMPGKFSLYPDLSIEENIEFFAALF
                     GTTVQENYHLVSDIYSQIEPFKDRPAGKLSGGMKQKLALSCALIHKPKLLILDEPTTG
                     VDAVSRKEFWGMLKNLQKKGISILVSTPYMDEAALCDRVALMQSGKILQIDTPQAVVN
                     SFNEVLYAVTSDKVYKMKEYLKHSNYCKNVHLFGQEVHVTLNTGVIDNVVTELKNTGF
                     SNVEIKPINPTVEDCFMKLMVQ"
     gene            complement(38643..39524)
                     /locus_tag="PK252_01135"
     CDS             complement(38643..39524)
                     /locus_tag="PK252_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT13338.1"
                     /translation="MRIIKFVAMISVLAFVSCNNRNGKTDAYGNFEVVETQITSEVQG
                     KITELEFDEGQEVKQGDVLLSIDSVSTVLRINQLEAQIESARARKTQVASQLDVYDEQ
                     LRAAEVNKIRIEKLVSDGSAPVKQLDDINDQLKLIAKQKNAVATQMHSIDAEIKALQW
                     QIAQIADQLSKYKVVSPVNGTILDKYVEKGEIITPGKVICKIADLKVMKLRAFISGSQ
                     LSQVKIGQKVKVFIDGANESFQQYDGQVSWISSKAEFTPKIIQTRDERVNLVYAIKID
                     VPNDGSLKIGMPAEVKF"
     gene            complement(39571..40866)
                     /locus_tag="PK252_01140"
     CDS             complement(39571..40866)
                     /locus_tag="PK252_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT13339.1"
                     /translation="MRRLFKIPVVLLFAFVPTFIYGQAISLEYCKQRVRQNHPIGPNA
                     TITDSIACLKGKNLKNEWLPQLNLNGQATYQSDAIKLDMLLPDTFQKPIGFSKKTIET
                     DRDQYKVWLDATQLIYDGGLNKARREFSDLSYKSDVFSAKSDINKVLDQTTQLYYSII
                     LNRSNKKLVEVLINTYDTKLKQINSLISQGMMMESDRDNITVEVLKLNQQLFELDMLY
                     KSLLANLSVLMGEQIGDSAKFDIPVINKPDSLQMSRPDLAALDAKQEALQYSEKIYKA
                     QRMPKLSAFAQAGYGKPGLNMLSTSFDPYYIIGLNFKWNIFDWNNSARERSIARMQAV
                     MINNQRSAVENNIQISINNSKSRIVQLENAVVTDAKIVELRSSIARRSADRLDKGLIS
                     STDFINDLNAEMQAKLNLEMHKVQLMQEQSNLQSIYGVY"
     gene            complement(40869..41495)
                     /locus_tag="PK252_01145"
     CDS             complement(40869..41495)
                     /locus_tag="PK252_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT13340.1"
                     /translation="MTDEQRLKTEEQIFAAAMEVFFEKGYYGARMQDISDKAGINKAL
                     LHYYYRSKDKMFEMVFSKTIDKFFENMAFTLSKDMSLFERIEVAVDNYISFFSDNPHI
                     PVFVVQEINRDSEAFKKLMIDKIDMLRNNVMAPFVMSIQENIAKGVIRPIDFRHLIVN
                     IVSMCIFPVIGKPLIQGVVFANNSGDWNLFIEERKKAVAEFVINAIKK"
     gene            41935..42609
                     /locus_tag="PK252_01150"
     CDS             41935..42609
                     /locus_tag="PK252_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13341.1"
                     /translation="MKTSLKVMVVAVATMVSGAVSLAQTTVTPGLDLYSTYLWRGSKI
                     DGPSAQPSLVVKNGGLTVGTWGSFGLASSYSEIDFYVSYAFEFGLSLGVTDYYYPTLN
                     ADGYSAFEFSKDSGAHAIEANVGYTLDKFTLSANYIFNEAAAAASTGGDKYFEASYAF
                     ESFSLFVGAGDGWHTKDTKFALCNIGAKATKEIKVSDSFSIPVTGVLMINPDKELISL
                     GVGISF"
     gene            42720..43067
                     /locus_tag="PK252_01155"
     CDS             42720..43067
                     /locus_tag="PK252_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT13342.1"
                     /translation="MKKIEAIIRKSKFEDVTNALHEAEIDFFTYSEVVGVGNEKSGAI
                     YRATVYETRFIPRILISFVCRDHLAENAVQAIATAARTGELGDGKIFISTVDDSVRIR
                     NGERGPESLFIKD"
     gene            43090..44316
                     /locus_tag="PK252_01160"
     CDS             43090..44316
                     /locus_tag="PK252_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT13343.1"
                     /translation="MDSTALTIGLNTIWVLIAGALVFFMQTGFALVEAGFTRSKNTTN
                     ILFKNLMDFCIGTFVFWLLGYGIMFGAGNGFIGGFELFSKVDHGAAAGLPNMAFFFFQ
                     LVFAATAATIVSGAMAERTKFSAYLIYSAIISLIIYPISGHWIWGGGWLSQLSIPMHD
                     FAGSTVVHSVGGWLALTGAIVLGPRIGKYKNGKANAIPGHSITLATLGVFILWIGWFG
                     FNPGSTLGVGDAGLISKIFVTTNAAAAVGGIATMILTWFRYGKPTLSMTLNGVLAGLV
                     AITAGCDAVTPGGAVIIGFIAGIVVVFSVELFDKVFKIDDPVGAISVHGICGALGTLL
                     VGIFSQSGGVLYGGGWGLFTSQAIGVVSVAAWTIAVGFILFLAIKYTIGIRVSAKEEE
                     IGLDFFEHGETAYNNH"
     gene            44909..45265
                     /locus_tag="PK252_01165"
     CDS             44909..45265
                     /locus_tag="PK252_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT13344.1"
                     /translation="MKKIEAIIRKSKFEDVKAALHAVDIDFFTYWDATGVGKSTEKII
                     FRGKSYDTSKINRTMLSIIVRDQNLEKTINAIITSAKTGEMGDGKIFVSDIEDVYRIR
                     NAEKGPEALYIKGEEV"
     gene            45306..46727
                     /locus_tag="PK252_01170"
     CDS             45306..46727
                     /locus_tag="PK252_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT13345.1"
                     /translation="MKTLSIKTIGLILLVFFCTLGVSAQDPKAANDSIKATTEATIAP
                     VSPSVDSGDTAWLIVAAAFVLMMTIPGLALFYGGLVRRKNVLNVLMQCFIITAVITIE
                     WVVIGYSMSFGSSEGILNPFIGGFDWAFLKGIHVSDLSPYFISHSQPTADGGTIGTIP
                     HIVFILFQCMFAIITPALILGAYAERIKFKGFLLFTVLWSLIVYNPVAHWVWSGDGWL
                     FKMGALDFAGGTVVHINAGIAALVTALMIGKRKYYEGHPTQPHNIPYVVMGAALLWVG
                     WIGFNAGSGLAADGLAASAFLVTHLATATAAFTWVILDWIFNKKPTVVGIATGAVAGL
                     VAITPAAGFVDVTGALLIGMLVSVFCFIMVGVIKPKLGYDDSLDAFGVHGIGGLWGAL
                     ATGLFATTAIQGSYSGLFYGNATQFIIQLKAVGVTILYSGILTAVLFWITDKLVGVRA
                     KDVDETEGLDITQHGEIAYNKAD"
     gene            46976..48334
                     /locus_tag="PK252_01175"
     CDS             46976..48334
                     /locus_tag="PK252_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT13346.1"
                     /translation="MKKRIWGIILLLVFLNLFQQNLFCQSVSQQEQASSISLGNTAWV
                     LISTALVMLMTIPGLALFYGGLVRRKNLLNIIMQCFIIMLIISIEWVVLGYSMSFGSS
                     EGFLKPFIGGFDWAFLNNIKASDLSPYAVTMPNARIPHVVFIMYQCMFAAITPALIIG
                     AFAERIKFKGFVIFSLLWALFIYNPVTHWMWSSDGWLFKMGALDFAGGIVVHITAGIS
                     ALIMAIMLGWRRYYNNTPTPPHNIPFVAIGTGILWFGWFGFNAGSGLAADGVAGNAML
                     VTHIAAATAGFVWALLDWFINKKPTTIGVCTGIVAGLAAITPTAGFIDVRAAIIVSTI
                     SSITCFIMVGYIKIKLGYDDSLDAFGVHGIAGIIGSLLVGIFASPAIQGSYQGFWYGN
                     KHQLTVQLIAVGATIVYSAIITFLLYKLVDKFFGLRVNPNEENEGLDFSQHNEIAYNE
                     TD"
     gene            complement(48603..48929)
                     /locus_tag="PK252_01180"
     CDS             complement(48603..48929)
                     /locus_tag="PK252_01180"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HOT13347.1"
                     /translation="MMKVIQMTKQFLLVGSLFLFAFVSCQTKKSLKVESFVVDEGWGY
                     KLVFGKKTIIYQTNIPAIQNQQTFHSERDALVIGYLVKEKLLHNQIPPTVSLNELASA
                     KVQIMP"
     gene            complement(48904..49935)
                     /locus_tag="PK252_01185"
     CDS             complement(48904..49935)
                     /locus_tag="PK252_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOT13348.1"
                     /translation="MMKYLKFGVLIVALVSLFTACSDDDDDSEDLIGNWVRMSDFDGV
                     ARSEAMSFVIGNKGYICMGYNGKNRLKDLWEYNNDGDYWTQKADFTGTPRSAGVGFSI
                     GSIGYIGTGYDGVNYLNDFWAYDPSSNSWTKKADLIVNGDTTLGRSDAVGFALNNYGF
                     VGTGYNDRWLKDFYRYDPATDSWRQITSILGTKRMGASSFIIGGNAYVVGGYNNDTYN
                     YDFYMFTPQGDSGVWTKKRDIKDSNKDESYDDNYKIIRAYAAAFSINSKGYLTGGSNG
                     SILSDTWEYNPTTDLWVKKSYFEGAARQRAVALTFSDKAIVATGTSSSYRFDDCWKFM
                     PDDESNTND"
     gene            50210..51505
                     /locus_tag="PK252_01190"
     CDS             50210..51505
                     /locus_tag="PK252_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOT13349.1"
                     /translation="MKKQHVVFLFAFFYFLVHSCKSDDFSIGSEDFNSSIRSVFIDTC
                     TVTFSMVKSDSFVTSGLTTGWLGSYHDKYTGTLTATPYFTFSLPSYTAYEKTFFDSVV
                     LYVKCLNDYFGDTALIQSFRVYELDDNIETDKNDNLYSTNSFARKTKELANYKFKPQP
                     YKKKYLSVKLSDDLGKDMLDKFMSESDEVSDDTKFSNYFKGLVLVPDVSKSNNVVNIE
                     VSDTSVSMIIYYHRYEDKKKNETINISLNSSLFFTHIDHDKTGTNIEKLNSQYGELSS
                     LLTNNEVYLQSMTSTTVRIDFPYIKNLKQLGDYIHINSAILYLKPLKNSYPFLSPLPE
                     NIYLSYIDESDYKSDQVAFKLSRDLYFNDLNTNYSVSITDLIKNQINAMSIDKLRINL
                     NLKKDGSQRDLKRLVLGDFYNEKAKAYVEIQLSIYNDEN"
     gene            51492..52724
                     /locus_tag="PK252_01195"
     CDS             51492..52724
                     /locus_tag="PK252_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13350.1"
                     /translation="MMKIKRYYLASVFIATILLVNQPVQAQNSYNPNSAYGIGETNLG
                     AQGYNAGMGGVSIGVRSKDFINTDNPAAISILDSSVVLFNFAMNGKYSKFAATGNNQT
                     AWNGNIRNLGLGLKIARFWGTALCLLPYSSVGYDIYTLQPIEGSKYVEAANYIGDGGI
                     NRIQWINAFKIHPKFSVGVNASYFFGAINTRQMVNSWTIKKVSSVQKLYFDFGLQFHD
                     NISNDLSYTVGLVYGNKSELSFKNTIYTTVSSTEIEDRTTKSTTQYLPQYFGGGISFT
                     MNEKISIAADYQYTQWSSLNKTIANVKYRDVSQIKAGIKVGPLQEITTNYLNRIHYMA
                     GIAISNSYIERSGVNPKNFGITAGLGLPIRQKAIVTISAELGKTGTTEKGMVSETYVR
                     FGVGVSWKDIWFVRRKYD"
     gene            complement(52733..53638)
                     /locus_tag="PK252_01200"
     CDS             complement(52733..53638)
                     /locus_tag="PK252_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13351.1"
                     /translation="MRRLKLTFFVLLFSFVSLKAQRLDSISAINTINMFCDPGYITGL
                     GGVGNIEPLMFEADIIPYYMIGFNKDWRWGMGLSPRVMLRMYNAESYPVRTPSYMPRI
                     LLVRQFVNNAKKHDWFVYLSWYHHSNGQDGPFFNADSSAINTRNGSFSTNWLEAGLFL
                     SRNKENRKYYMKLYSEYSYNQDKELNGIYGRLRVYLDLKTEWNLVSKTLNLFNLSYFE
                     ENKDVLNNSFRFGYIATDLKNVNYWDKKRFTFKYTLTYKPSFLRDVNLFAQYYYGQDY
                     YNIYFYRTLHILRFGIAAKTSVFNR"
     gene            complement(53691..54143)
                     /locus_tag="PK252_01205"
     CDS             complement(53691..54143)
                     /locus_tag="PK252_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13352.1"
                     /translation="MYSIVDHLQLIEKYSPSGPCSCPTCVSYCKRPGWWTIDQACNAL
                     YRGYAARMMLEISPDRTFGVLSPAFKGNEGGVSLQIFSDNGCTFLQNNLCELYSTGLQ
                     PLECRFCHHSRRGEGIACHNDIGQEWNSANGQQLVNFWYRNYLLRKFF"
     gene            54244..55023
                     /locus_tag="PK252_01210"
     CDS             54244..55023
                     /locus_tag="PK252_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT13353.1"
                     /translation="MPVNLKQLTATHEQNRKSHQCIVSEEDYKMVEPLLKLLQRLSEV
                     ENSTISVFDMNRNNYLLKSSKFKEMLGYKTHEDIENDDTALFHQIIHPDDLPFVLETE
                     NKAYIFFNSLPNEEKKDYKLVYDFRVKNTAGVYMRFIHQLAVLEQDPFGKSWLIMIIT
                     ELIAQRALNVVSKRRMINIKTKKLHIFNEDDKLHADMLLTKRETEILNLIAQGLDSQR
                     ISEKLFISVNTVNNHRQKILNKTQTDNTSQAILYAKKMGII"
     gene            55036..55602
                     /locus_tag="PK252_01215"
     CDS             55036..55602
                     /locus_tag="PK252_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOT13354.1"
                     /translation="MAYVEEKTFNKVDFTSKALEKGEYDNCTFKECDFSNSDLSDIKF
                     LECRFDNCNLSMTKLINTSLCDVRFKNCKMIGMHFETCNEFGFSIHLEDCNLSLSSFY
                     KIKMKKNTFKNLKFHECDFTESDLSLSVFDQCDFAKATFDNTNLEKADMQTSFNYSID
                     PDKNRIKKAKFSLNGIAGLLDKYDIEIN"
     gene            55755..57689
                     /locus_tag="PK252_01220"
     CDS             55755..57689
                     /locus_tag="PK252_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13355.1"
                     /translation="MKQIILSISLLIAAKCMAAPADTIPANSSLIEYTGRIDFSNPLA
                     PKFSYSGVSIRACFTGTSIAAIMNDNIGKNYYNVILDGKVTDTINVSIGQKTYLLAEG
                     LENKVHEIELFKRTEQEFGKTEFLGFVVDKDANLSTIVNQREKMIEFIGNSITCGYGN
                     EGKNGEKFGPTTENHYLTYAAITSRNFNARHLAVCKSGIGIYRNYDGPATGDAECMTN
                     LYTRTFLYDDKPKYSFAQKPDLVCIDLGTNDFSTTGCDSARFINNYFRLIDTIQTKYA
                     MPEIICLAGPMMSGSALVTIKKYLTFIADSATKKGKGKVYFFEMSAQSGDVGIDYHPN
                     VTQHKKNAQELTDFIVKTKGWKITPLAASASLIGAKHIQVVFNTGIQDSTNNFTGIAV
                     AGNNGNVSIDTAFIDTADNKILHILTESSFAIGESVTFSYNQGSIMSIDSIALLTINQ
                     YTIQNKLTETKLARGISTTDGKSILLSFNKALKNNNNIEGLTITNSKGILAIDSFAIS
                     GTQINLYLKEALIKGDSIFANYNGNAIVGFDDIPLESITKLVVKNASKIVSITNMKNT
                     TVEIFPNPNKTGKIYYRINLTSGNKGTIDIFNINGKLVFSQSISKTEGFIDATNLKKG
                     SYIVKIKQGENSISKTILLQ"
     gene            57747..59345
                     /locus_tag="PK252_01225"
     CDS             57747..59345
                     /locus_tag="PK252_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT13356.1"
                     /translation="MANTKKMHPLAWLMLFMLCMNVGAQNPNFYIYLCFGQSNMEGQG
                     TIETQDKTVDSRFKVFQALNCSNLGRTKAKWYTAVPPTCQCYSKLSPADYFGRTMVAN
                     LPDSITVGIINVAVGGCDIRLFDKDIYMDYDSTYKESWFTSKVKDYGYNPYKYLIDLA
                     KLAQQDGVIKGILLHQGETNTGDAQWPTYVKKIYNDMLADLSLKADSVPILAGEVLAA
                     AGNCCSSMNSIIKRLPDTIPTAHVISSAGCAGQDYAHFNSEGYRKLGRRYGATMLSLM
                     GLEAAYAEAECGTVGKNWKTLADKNASNGAYVTQTAAQNNTASAPSSEDNIIQMSISV
                     SADTSYYLYGRFNNASANSNAFWVKVDDAEFELFDNLTTTGWQWLELKKLDLTAGEHK
                     IYVGFAKDSAMLDKICLKNAQKLPVDVAEEAANLCTPSITPDPTQVGVTAKDGYALYQ
                     NFPNPVNNCSTIITFHIPQTSFVSLKVYNSQGLEIKELAGRTFTSGEHNITFNIEKLS
                     PGAYFYTLRAGQFSSTQKMIIPVE"
     gene            complement(59763..60704)
                     /locus_tag="PK252_01230"
     CDS             complement(59763..60704)
                     /locus_tag="PK252_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT13357.1"
                     /translation="MNFNVNNMQSLKSKILLNLMLHSHLFRFQLKRKPFDPSMEGIRK
                     MRERVEQAANRFGKLPKETVIDELNINGRYAEWVRLPGNHAEKAMLYFHGGMYLLGSP
                     QSHRQHVVKFVQGTGVNALVFDYRLAPEYSYPSALEDALAAYQYLLDEGYKPSNIIFA
                     GDSAGGGLCLATLLALKDKGIALPAAAAALSPWTDLLLTGRSYKTNMKKCFAPIGCPQ
                     YASEFYAKGHDRSNPYISPLYGDLKGLPPLHISAGGNEILLDDAICFAQKAKDAGVPV
                     TLRVDKGMCHCYPAFGNLFRESKLALAEICEHLRRYV"
     gene            complement(60694..61302)
                     /locus_tag="PK252_01235"
     CDS             complement(60694..61302)
                     /locus_tag="PK252_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT13358.1"
                     /translation="MPRNKEANQKVKDGRREQILAAALELFARKGLVATRISDISAKT
                     GISQGLIYHYYKSKEEVFNWLIRDAVEKMNTAALELEKLPLSAKEKIVLAVEQLLKGF
                     DQKVETAYYYFLITQAALSEAFPQEARDFIFSQNNIKYGVINRIFIHGQKEGTVRSFD
                     AAEMTTLFFSTINGLAMYKAINGANAKMPDKRIILNMFLNEF"
     gene            61440..63233
                     /locus_tag="PK252_01240"
     CDS             61440..63233
                     /locus_tag="PK252_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOT13359.1"
                     /translation="MNNLNYGVIGNCRSAALVSSTGSIDWCCLPDFDSPSVFAKILDK
                     EKGGYMAFEVDDSYKVSQKYFHRTNVLCTEFKSDEGTFEVIDFMPRYKTAENQYFSPS
                     EIYRYIRPISGAPIFKVKYAPALNYAREKVAHNIEDNRVRSYSVSNPTDCIYLYSSID
                     FEAILNSTPIKLEKQQFLLVSYNQKLIDIDINRVYLEYQRTKVYWLNWTNRSKKYEEY
                     TEEIIRSLLVLKMMTYQPTGAILAALTTSIPETIGEVRNWDYRFCWLRDASMAIDTLL
                     KMGHYNSAKGFMTFIKGILKSKHDTFQIMYGIRGERNLTEYNLMHLSGYENSKPVRIG
                     NAAYYQRQNDVFGYLLNVIYQYYEYFPGTLDEIEDMWEIVRNIARTVSTHWEKPDKGI
                     WEIRNNEKHFVFSKVMSWVAMDRATKIATILNKPYYYCNWREIANDIKNDVLKNGWKK
                     EIQSFSQAYDNTELDASLLLMAEYGFISHTDEKFINTVQAVKKALSHQGLMYRYKNED
                     DFGNPSSSFTICTFWLIQALYQTGQAAEAKNIFDNLLQWSNHLGLYSEDLDFETKRLL
                     GNFPQVYSHLAFINTATMFSKEKIISRFIKP"
     gene            63284..64603
                     /locus_tag="PK252_01245"
     CDS             63284..64603
                     /locus_tag="PK252_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOT13360.1"
                     /translation="MGAKSVIRKVLHFFNLESELEEQDSVYEEIKKGIIFKGTNLWIL
                     IFAIIIASIGLNMNSTAVIIGAMLISPLMGPINGMGYSIATYDFELFKRSLKNFAFAI
                     ISSLVASTAYFALSPISTAHSELLARTSPTIYDVLIALFGGLAGIVAISSRQKGNVIP
                     GVAIATALMPPLCTAGYGIATGQLDFFLGAFYLFTINTIFIAIASVWISQLLNFPIRT
                     IIDEKKKKHINRIITLIISIVLLPSIFFGYKLVKQEEFIQKTNQYISGISNFEGNYLL
                     NHKIDEKSHEITLVYGGTSLSDEQKDAIIMKAKDFGLEETIIHIEQGISLGNKDNEKG
                     ELFRLKEEIITLNSTLKKKKATLDSIQNSHQTGQVLLNEIQTIYPQIKNCSYSKTYTY
                     SEKHASPTSTTIIVLTTKGSITVSEKAKINNWLKKRLNNEAVKVFYE"
     gene            64621..66840
                     /locus_tag="PK252_01250"
     CDS             64621..66840
                     /locus_tag="PK252_01250"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOT13361.1"
                     /translation="MEANFKRLVIAAYRLPFKFSKTKNGYKTMQNSGGLVSAILALSE
                     KFKKSKDFRVNEKILWAGIDNNIPDDFPSDKLNNKHFNICPVHIPANTNELYYGGFCN
                     DLIWPLFHYFPTYSVFNNNYFKAYEEANTLFCNEIIKVIKPGDFIWIHDYQLLLLPEM
                     LRKKMPDVTIGFFLHIPFPSFEIFRLLPRKWRESIINGMLGADLVGFHTHDYTQHFIK
                     CVKRTTGFECHQNVILTPNKLVKADAFPIGIDFDKFHGACFDKPVVKEKEQIKKQLSG
                     QKLVFSVDRLDYTKGLLLRLKGFETFLEKYPAWNGKVIFNMVVVPSRDNIKQYEKMKK
                     EIESTVGRINGKYSSLSWRPVIYQYKSLSFNELIALYDLSDVGLITPLRDGMNLVAKE
                     YIACQKENKGVLILSEMAGAAAELTEAIIINPVDNDEMAEAICSALEMKTNEKHVRLK
                     RMQDRIANYNVFTWALDFFAQTADAKIQQNQMSVKYVNKQIVSQIKTSYLNAAKRILF
                     LDYDGTLVSFAKYPELAIIDKKTLSIINQLSKDPKNTVVIISGRDKNFLEKQFEGLDV
                     TLVAEHGYFEKKHGKDWKETVNTASNWKSIVMPILKEYVNRCNGSFIEEKTGSLAWHY
                     RNAEMDFAQLRLHELRDDLAEIIRHKTDLEILEGNKVLEIKSGKYDKGQTAANLLKNK
                     NYEFILAAGDDKTDETMFSALPAETFSIRIGKCSSLAKYNVGSYIEFIALLANLSDM"
     gene            complement(66861..68012)
                     /gene="chrA"
                     /locus_tag="PK252_01255"
     CDS             complement(66861..68012)
                     /gene="chrA"
                     /locus_tag="PK252_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOT13362.1"
                     /translation="MKEHQPSYIELFRAFFWLGLTAFGGVAMSAHVRKHIVEKKQWLD
                     ANTFDSGLALCQVIPGAIIMQLVAYIGLKLKGSRGAIVSFIGFGLPAFLIMFILSVIY
                     KHSKHLSNVELVLNGLRVIIVAIVANAAYMFGSKNFENYNDWIIASIAAGLFLTKLHP
                     ALVLLIAALLGIVFTKKDFGESKKRVKSKSFKLFIWTLAVVAVATGIIFLVNRDYFTL
                     ATIMLRIDLFSFGGGLASMPIMYHEMVDLYGWFDQKTFMDGVILGQVTPGSIIIAATF
                     FGYMHFGILGSILATVCVFIPSFLILMGLIPFFDKLKAYPQFNKVINGVLCSFVGLLV
                     VVTYRFTIGVQWNIFNIVIVLVAFVLLLRKVDVIWIVIGGMVLSFLVMY"
     gene            complement(68017..68940)
                     /locus_tag="PK252_01260"
     CDS             complement(68017..68940)
                     /locus_tag="PK252_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOT13363.1"
                     /translation="MRNSWIIYVTAVVLCLFPFVDAPIALFMGIILAQTVKHPFLLTS
                     KKLTHILLQVSVVGMGFGMNTFQAAEAGKQGLVFTIFSIATTIGLGIFLGKKMKVERK
                     TSYLISTGTAVCGGSAIAAVSALVKSNEKQVSTALGTVFILNSLALFIFPIIGHWLNL
                     SQHQFGVWAAIAIHDTSSVVGAASNYGQEALQVATTIKLERALWIIPIAFLTVFIVKG
                     DNKKVKIPYFIILFVLAMLINTFIPAVHHEAAFIVNISKIGLKITLFLIGTGLTRDLL
                     KNVGIKPFIQGILLWFFISVLSLTIIMYSLL"
     gene            complement(69009..69908)
                     /locus_tag="PK252_01265"
     CDS             complement(69009..69908)
                     /locus_tag="PK252_01265"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOT13364.1"
                     /translation="MLDFRLNVFICVAHNLSFSKAANELYITQPAVTKHIKELESEFE
                     AKLFNRHGNKIELTKAGILLLSYSEKIQSLHNEVKFELSRLKGKSKGELRIGASTTIS
                     QYVIPSALSKFHERYPDINLSLVNGNTLQIEKRLLKNDIDFGIVEGNPGNTDIRYYPF
                     LDDELIVFTSSQNKSAPQVVSAKELASLPLVLRERGSGTLEIIENSLQQYGVNLKSLQ
                     VLMYLGSTEAIKSFVKTGSGVGIVSRFAIEHELLSGIFRQINTSNIRFQRQFYFISPQ
                     GPEPSGLTKLFVNFLQNYYNLKL"
     gene            complement(69980..70369)
                     /locus_tag="PK252_01270"
     CDS             complement(69980..70369)
                     /locus_tag="PK252_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT13365.1"
                     /translation="MKIKALIAIVTLFISTFATLTAQSPIESNSMEVSTMLKKDKKLV
                     VLDVRTAGEFQAGHVKGAVNIDVTQQGALEKINKLDRKARYIVYCRTSRRSKIATDHM
                     VQNGFKTVYQMMDGFSGWTANNLEVVK"
     gene            complement(70457..70663)
                     /locus_tag="PK252_01275"
     CDS             complement(70457..70663)
                     /locus_tag="PK252_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOT13366.1"
                     /translation="MKKNMGTIDKVIRILVAVIIAILFFTNIISGALGIVLLILAAVF
                     VLTSLISFCPLYIPFGIDTCKKKE"
     gene            complement(70715..71158)
                     /locus_tag="PK252_01280"
     CDS             complement(70715..71158)
                     /locus_tag="PK252_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HOT13367.1"
                     /translation="MDIANYFKYNELLSSGAQPSTAQIEALKAAGFEAIVNISPVSTR
                     NALKDEAQVVEKLQMDYVHFPVDCSNLRPFHYNTFSGIMAGLDGKKVFVHCGGNIKSS
                     NLIHMYHVLEKGIDEAESLKTLKQIQNPEEKWFSYFKSFGMQGMQ"
     gene            complement(71165..71620)
                     /locus_tag="PK252_01285"
     CDS             complement(71165..71620)
                     /locus_tag="PK252_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HOT13368.1"
                     /translation="MNEITLYSASDIQTMQLADQSRLPGKNRVEEIRNFAKNAGFKRI
                     GIANCVAVQKEANMLKESLSKDFEVFAVDCKCGKIPASEFLGEEAKGVSCNPAGQADF
                     LAENKTELNVVLGLCLGHDMIFTAKSKAPSTTLIVKDREFKHNPMEIFK"
     gene            complement(71628..71813)
                     /locus_tag="PK252_01290"
     CDS             complement(71628..71813)
                     /locus_tag="PK252_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT13369.1"
                     /translation="MALVVNPQACPQNHRCPMLRVCPVGAITQDGFGLPVIDEEKCIE
                     CGKCAMICPMKAVTSKN"
     gene            complement(71820..72119)
                     /gene="trxA"
                     /locus_tag="PK252_01295"
     CDS             complement(71820..72119)
                     /gene="trxA"
                     /locus_tag="PK252_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT13370.1"
                     /translation="MKANFNSLINDTRPVIVDFHALWCAPCKAQSPILKEIATELGDK
                     VRVIKIDVDQNPDIAGRYHVQGVPTLAIFKDGELKYKQAGVHSKNQLMNILLNIK"
     gene            complement(72344..72760)
                     /gene="trxA"
                     /locus_tag="PK252_01300"
     CDS             complement(72344..72760)
                     /gene="trxA"
                     /locus_tag="PK252_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT13371.1"
                     /translation="MQTTLIVLGVMIGVFAYLVFAAKRRLKNIPMVKDHDKILTLTEA
                     NFQQQTKGRVVLVDFWASWCAPCRMMAPILNDVAGELSGNQHVGKVNIEQYQSLAQKF
                     KVRNIPTLILLKNGVEVNRFVGVKSKDFLIKQMKQV"
     gene            complement(72856..74124)
                     /locus_tag="PK252_01305"
     CDS             complement(72856..74124)
                     /locus_tag="PK252_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT13372.1"
                     /translation="MKEINASEFETEVLQGGKVVLDFYSTECPPCEALAPKFENLSRL
                     YGNDIKFFKIFRQQNRELADKLGVKSSPTLLFFDNGKEVADRLSGGIKRSDIICHLDS
                     MIPVERVKAIKSQIKPAVTEFDVIILGAGPAGLTAGLYLCQAKINTVMVDIALPGGHV
                     STTHEVSNYPGFIEPQAGYMLSHNMSEQTKLCGSVYKVAVDVTRVDLEKKEVVIDEYE
                     TLKAKRIIIATGTSPNLTGAPGEMELKGKGISYCATCDAKYFVDKEVVVIGGGNSAVE
                     ESDFIAKFASKITMVHQFDKLTANKKAQEKLLANPKIKVLYEHEPRSFVKEGNQVISE
                     IENLKTKERIKLVSDGVFIFIGFKPNIDLFKEKLELDQWGYIKTDEDMRTSIPGVYAV
                     GDVISKKYRQITTAVADGTIAAISIAKELD"
     gene            complement(74219..74593)
                     /gene="trxA"
                     /locus_tag="PK252_01310"
     CDS             complement(74219..74593)
                     /gene="trxA"
                     /locus_tag="PK252_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT13373.1"
                     /translation="METMVKDVVYLTTQEFKEKVFNYEVNKEWKYVGELPAIIDFYAD
                     WCGPCKMVAPILQDIAKDYAGKLIVYKVDTESEQELASVFGISSIPTLLFVPKEGQPQ
                     AAMGALPRQTFDQAINEILLKN"
     gene            complement(74699..76018)
                     /locus_tag="PK252_01315"
     CDS             complement(74699..76018)
                     /locus_tag="PK252_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT13374.1"
                     /translation="MVFSKHNIFSNIKDSENFFIVNLLSGNADILSPDEACKIDGLRN
                     SKEIVSDDFVNDMVAKGYWVDETEENKLYKSKYLDFIDNRDNDEVQLFFVPNYSCNFA
                     CSYCYQDEYAPAKSELTNEIVDAFFTYIQKEFAGRRKYITVFGGEPLLNSPKQKEIIT
                     YLVEQSNKVGLELCFVTNGYTLAEYIDILKKGRIREIQITLDGTAAVHNVRRFLKGGA
                     GTFDKIVNGIDVTLANKLNVNLRMVIDKENINNLPELARFAIDKGWTKNSYFKTQIGR
                     NYELHHCQSAPDKLFTRVSLYESLYSLIKQHPYIVEFYKPAYSISKFLAENGSLPAPL
                     FDSCPACKTEWAFDYTGHIYPCTATVGKADESLGTFYPQTALNTSLINEWEKRDVTTI
                     NDCKDCSLQLACGGGCGSVAKNQSGQICSTDCRPVKELIELGFASYF"
     gene            complement(76563..77012)
                     /gene="trxA"
                     /locus_tag="PK252_01320"
     CDS             complement(76563..77012)
                     /gene="trxA"
                     /locus_tag="PK252_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT13375.1"
                     /translation="MRKIITLSVLAFAFLLGNCSAGSPEGKAKFGEVIVLTNETFKQK
                     VFNYEVNKQWKYEGTMPAIIDFYATWCGPCRQISPRIEEIAREYAGKIVVYKVDVDVE
                     RQLAQNLGIQSLPTILFIPAKGQPQAAMGALPKESLVKAINEVLLVK"
     gene            complement(77035..77682)
                     /locus_tag="PK252_01325"
     CDS             complement(77035..77682)
                     /locus_tag="PK252_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT13376.1"
                     /translation="MSLIEINKRYSELAKSDCCLSCGGAINYSEVKAGEVCVDLGSGR
                     GTDVIRLAESVGENGFVYGIDISDGMIEKAIDTAKRLNVTNVKFVRSELEKIALDDAV
                     ADLVISNCTINHASDKQMVWNEIYRILKKGGRFVVSDIYSTSPVPDEYRNDPVAVSEC
                     WAGSVTRDEYLSQLENAGFSSVNIIEESAPYDKGKIKVVSWTIAAQKPAKCGCKK"
     gene            complement(77747..77992)
                     /locus_tag="PK252_01330"
     CDS             complement(77747..77992)
                     /locus_tag="PK252_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOT13377.1"
                     /translation="MENNCEVKPRPKTIKEFFSSWYFWKPFIATVIGMIAGFLYYYYI
                     GCSSGSCPITSSPYMSIIFGGFMGFFIVSSPCSRGKC"
     gene            78121..78555
                     /locus_tag="PK252_01335"
     CDS             78121..78555
                     /locus_tag="PK252_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT13378.1"
                     /translation="MSKVFDDSLSPEQLEEMFHNDEKCLEFLANLKWANGFVCKKCGN
                     TNSCPGKEPFSRRCTKCKTKETATNGTIFHGVKFPISKAFYIAYNVCKGKEDISSYEF
                     GRRLSLRQMTCWNFKSKIEHALVEMGSLTDKERSSIEKILTS"
     gene            complement(78591..79670)
                     /locus_tag="PK252_01340"
     CDS             complement(78591..79670)
                     /locus_tag="PK252_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13379.1"
                     /translation="MIKKGFITFCLVTLPFVTLSSQDTTAISWDGFFESAAFAQPFVS
                     EMHSNVSKAELGYNKSYSEFDLLEEGSKFNRPMVELHAGFDIPLFANFSRDIDNKNKW
                     GFAASVPVSVHVLEDMWGSETAPVINTDYRFGSFKLRLIRYFPNNRFIKNISFSWLPI
                     FHECTHLGDEITIYRKDENFPITRINVSYEYTEFQATINDPDRIRENCHSLRLGFSYR
                     ISNRGLGWFSVRKDAELTTDLDIPASKYRGEYYLEYQFQRTQGFLASKRFVNVLSFEV
                     RYRLRYGYPLFKKVDEGKWETTEVKEGMLFNFNLYWGYKFYPKTNFNHAVGFYFHGYR
                     GLNPYGQLRNYPGYPFFGISLIYEL"
     gene            79810..80391
                     /locus_tag="PK252_01345"
     CDS             79810..80391
                     /locus_tag="PK252_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT13380.1"
                     /translation="MIDSEVIQKILEGKRDSYRILVERYQSMVYRTCLGFLHNQHDAE
                     DLTQEVFIKAYQSIDSFKGDSAFSTWIYRIAVNASLNKVRANSKNFLFQKLDTLLGSG
                     KNYEPSVVFHESENPEDMLVKEEHRLWVQKALDSLPENQRTAIVLSKYDELSQKEIAE
                     IMNTTEGAVESLIQRAKTNLREKLFAHLKKNKK"
     gene            80440..80856
                     /locus_tag="PK252_01350"
     CDS             80440..80856
                     /locus_tag="PK252_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13381.1"
                     /translation="MNCDLCKRELEAYREGRLPDGIRAQVEHHLHNCHDCTLYYHALI
                     AAEKVMDEEKSAQHNPFLSTRIMAGIEALESSQKRVRQGILKPVLISISIVIAVFIGI
                     TMGQLYKPAKQSNVIPAELTYMDDASMESLNVFVTE"
     gene            80867..81430
                     /locus_tag="PK252_01355"
     CDS             80867..81430
                     /locus_tag="PK252_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOT13382.1"
                     /translation="MKAENKHTTMVWAIVVLAIMNISTFVTIMYHQHKAETIIPNDVK
                     TPKLEAVAEKYSGRYFKEQLGFNSEQMDKFREFNRPFRMQAREITLQLADVRKAFLQE
                     IAAPNSDTAKLNALSDSIGEMHKQLKKLMFQYCLDIKTICNNEQKEKLNQLFQDIFIN
                     ESPMALPGHGPMKGMKKGMEQGQCKSQ"
     gene            81479..81772
                     /locus_tag="PK252_01360"
     CDS             81479..81772
                     /locus_tag="PK252_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13383.1"
                     /translation="MKTKLILSGIALFAAISLANAQGNCCAKAKYNKDSKGTGYVDAN
                     KNGICDNYENRADTTSVGKGKGNGKGKGNSKGKNFVDNNKNGICDNYEARNKK"
     gene            81919..82710
                     /locus_tag="PK252_01365"
     CDS             81919..82710
                     /locus_tag="PK252_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOT13384.1"
                     /translation="MKTTFSKIVTGSFIAIAAMTMSSCEKDLSNSENMRYASIIDVAG
                     DGTTSVMHSNMQQAFVETAALTETELASLVKIKDEEKLARDVYAALYQKWASPVFSRI
                     TNAENNHLSAINLLLAYYGSSDTLIGEVGVFTNAEVQALYNNLVSAGSTTIEDAYKTG
                     ALIEEMDINDLQNVIATTSNANITMVMENLERGSRNHLRAFNKQLVTLGITYVPTYIS
                     QSAYDQIVNSSIEKGKNYMMQGNGKGHGKGKGKMRNGNGDGSCNN"
     gene            82953..85298
                     /locus_tag="PK252_01370"
     CDS             82953..85298
                     /locus_tag="PK252_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13385.1"
                     /translation="MPKISKIAALTFILWITSVMSVFSQAKFTISGEIKDSKTGETLI
                     GATVYPSEMPTKGVVTNAYGFYSITLPEGKYNLVSQYTGYEKSQQTIDLKQNMKVNFE
                     LGESVISLNEVVVTAEKQDRNITSTQMGMNKLNINEVKNIPVLFGEKDVLKTIQLLPG
                     VKSAGEGNSGFYVRGGSSDQNLILLDEAPVYNPSHVMGFFSVFNSDAIKDLNLIKGGM
                     PAEYGGRLSSVLDVKMNEGNNKEYHVNGGLGLISSHLTVEGPIQKDKSSFMISGRRTY
                     ADIFLKASSNEMLKNTNIYFYDLNAKMNFHLGEKDRLYLSGYFGRDDINLLRFSNSFQ
                     SMWGNTTGTLRWNHIFSNKLFLNTSLIYSDFTYNMQLGSDSTQVNVESSIRDYNFKQD
                     WQYYMNSQHTVKFGYNAIYHSFIPGSVTGNATTSLVSSDIPKRYALENAAYLSDEWAV
                     SEKLKITAGLRISNFNQLGPTTIYGFDEDGTITDTTVYKNNENIKTYWGIEPRLSASF
                     MLNESSSIKASYARTQQYLHLLSNATSYQSTDLWIPSSNIVKPQIGDQYAIGYFKNFA
                     DNLFETSVEVYYKTMQNQIEYKNGAQFVLNKTAEADLVFGKGKSYGIELFAKKRYGKF
                     NGWISYTMSYTDRTFADIDAGKTFAAKQDRRHEVSVVGIYQLNPRWSLSATWIYYTGN
                     AVTFPSGKYMADNRIVYYYTERNGYRMPDYHRMDVAATWIHKKTAKFESSFNFSIYNV
                     YNRLNAYSISFRQSETNPDQTEAVKLSLFPIIPSVTYTFKF"
     gene            85309..86136
                     /locus_tag="PK252_01375"
     CDS             85309..86136
                     /locus_tag="PK252_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOT13386.1"
                     /translation="MNTIYKLALAIPVIAAFSACEEIINIDLNTASPKLSVTAIITDQ
                     PGPYYVTLTKSESFFSSNDSFPAVSNAVVVISDDAGNSEQLTEIKAGVYQTNNLQGVS
                     GRTYHLSIEADGKKYEASSFLPYPVALDSVASQKITATSPGNKENKNKYYLKCYFTDP
                     KGSEDYYRVEPIIPNLDTLSSSYQIYSDEVTDGQHVEFPVQKPTFSFGDTAIVEFYHV
                     NKENYDYYKTANNILKSKKGPMASASAPQANPLTNISGEAVGYFGTFAVNRRLIVVK"
     gene            complement(86297..86524)
                     /locus_tag="PK252_01380"
     CDS             complement(86297..86524)
                     /locus_tag="PK252_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13387.1"
                     /translation="METTCKNFEKCPIYTGVLEGKDATASNYRKQYCDAGEDGWMKCK
                     RYLVKEKAGKCPPDLLPNSFKTVDEIIAAMA"
     gene            complement(86545..86976)
                     /locus_tag="PK252_01385"
     CDS             complement(86545..86976)
                     /locus_tag="PK252_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOT13388.1"
                     /translation="MKKTVFSFLVLAISFTNGFSQLFSACENVSAFDKSRKPDIGIVI
                     SSGDAETVWNALRLGIAAESKGDSVVVFVISKAVDVFMKDTSKYDIPKTSLEFLKMGG
                     DIYTCATCAAMRHTENVQMCTITSIYDLYEIVKRSKKLVTF"
BASE COUNT        28340 a        17888 c        15238 g        25629 t
ORIGIN      
        1 ccctggatag catgcaaaca ttcggcaatg gtattccttt taatcccata ttctgaagag
       61 ctcaacggat ggtcaacaat agggatgcct ccataaacct gtaccatctt gaaatgtaca
      121 aatgcaagta taaaatacaa ttcgccttcg tattggttga gtaatttcaa tgaatcggca
      181 ggaatattag aattactgct tttatatttg ttaattcctg ctaatgtaga gttagcgcgt
      241 aatatcgatt tatagttgta tgcccaaaag gcatcagaga gtgatttagg ttcgcttggt
      301 tgaattgtgc ccaaatcggc atgttggata tccaaaaagt cgttcccgcc ttgttcgccg
      361 ccagcttcag cttcatccga agcgatactc ccaaaaacaa ggtacatcat atcgaggttg
      421 tgaatacctg cgggctgaat attcaaagaa ccgtaagttc cggtaacagc ctggcctata
      481 ttagtaaggc tgttataaaa ggtttcttca gattggaccc cttcctgggt cgtatccagg
      541 aaactctcgc tgcacgatgc aaacaaggca gctaccagta ttatttgtat aaaatatttg
      601 atatgtttca taattctatt tttttgaatt ctataaactc cttaacacct taaaatgtaa
      661 tattcatacc aaaaaggtac atttttgact gaggatatgt accacggtca aagccccttt
      721 caagaatact atttgaaacg gttgtatttt cgccaacttc ggggtcaaat ccggtgtatt
      781 tggtaaatgt gagaaggttt tttgccccaa cataaaccct taacttttca atttttatct
      841 tctgggtcaa agatttgggc aaagtatatc ccaactggat atttttgagc ctcaagtatg
      901 agccatcttc tacataaaat gaagaaggta ataacactgc gtctatggct gctatacctg
      961 tattctcatt ttgaggttct gacaatcttt caggctgggc cagatttggg cttgtattcc
     1021 attttgaagg aatataacca ttcaacaaat ctggagataa attcaaacta ccattattga
     1081 tagtgtatga gttcactttc agcaagttga atattttatt cccgtagctg ccctggaaga
     1141 atgcactcaa atcaaaacct ttgtattcga ggttgaggtt aaaaccataa gtaaacttag
     1201 ggttagggtc tccaatgttg gtaatatctt cagaattaat aaccccatcg ttattaatat
     1261 ctttgtattt aaactggccc ggagcagcag tactggactt ggtattatac gttacagggt
     1321 taccatttac atcaaatcct tctacaacaa cagctcccgt tgcatccggg gcaacctgtt
     1381 tccatctttt caaagctgtt ttgtaccaca caaaatcagc ttctttatat atgccatcta
     1441 cctgatatcc ataaaattca gaaactgcat gtccattctg agtgcggcat acgtttgaca
     1501 cacccctgcc ggttcctgcc tgcaacatat ctccaatatc gtcaatgaca gttttggtat
     1561 gggtaatgtt ggcactcagg ttaactaaca aatcgccaat ctgttcttta taacccacag
     1621 taagttcgta accactgtta tccaacgtcc catagtttac aagaggcctg gtatcgatag
     1681 agccatcgcc taattcctgc gaattgccgc caccaaggat tgcgtaccct acagtataag
     1741 gaactgattt tctaagtaac atatccttgt ttctcctggt aaaatagtca acagaaacac
     1801 tcaccttatt tttcaacaca gtaaaatcaa gcccaatgtt ttgagaaaca acagtttccc
     1861 aatgcaaatc ggcattacca gctgtgatca atgcagcacc actgtttttt acattattaa
     1921 aaacataccc gttaataggc aatatgccta ttgtactgtt gtacaaataa ggttgaatat
     1981 cagagttccc tgtttctcca taaccatacc tcactttcaa taagtcaaaa atattgaggt
     2041 ctttaattgc ttctatttcg ctcagtttta tcccggcaga aaaagcaggg aatgtaccag
     2101 ccctgttgtt tgccccaaac tttgaagaat aatctcgcct gatattggcc tgcaataaca
     2161 caatattggc aaagtcataa cttaacctgc caaaaaaagc ataccccctg attgtctttt
     2221 taatagggtc gtgtatgttt ttaaaagcgg tatcgttaag gtcaaaattt ttccagtctg
     2281 caacatccag gttaaccccc gaagctgatt tatctttaat ttttacaatg tactcgccac
     2341 tttcaaaacc tgctactgca ttaaacttgt gcatatcgga taaagacaaa tcgtaggtaa
     2401 gaaaaccctg atagttccat cctgtatttt cataagtgcc agtattatat gagttaatct
     2461 ttacactttg cgttgggtta gcagcagcgg tatctccaat ggtatggacc ggagtaaaat
     2521 ttttgtattc agaattgctg ctgttaagcg aataatgtgt ttcgaaattt aagccttcaa
     2581 taacattgag ttttaaaaat acatcaccat tgaatgacct actattattg acattattgt
     2641 tatactcaat ctgggccagc gggtttttaa cacctgaaga aaggtctgaa cctttataag
     2701 catagttgcc agtaccatct gttgagtaaa cagggaccat tggcaacatt tgtattgctg
     2761 tacttaatgg tgagttgtac tcgttcatct cattctgggt tgaattttcc ctttgcgaaa
     2821 agctaaaact ttcacctact ttcagtcttt tcattacctg ataatctgaa ttcaacctaa
     2881 atgtgtattt tttattgtaa gtccttaata ccgtgccgtc ttctccatta tatccaatac
     2941 ttgaatatac attgtatttc tttccgcctt tattgaccga aaggttataa ttctgcatca
     3001 aagcttgcga acggaagatc ttgtccaccc agttggtatt ggtcaaagct tttgtagaat
     3061 ctgaatggtc catatacacc ttgtttacat tgtcataagg gaaataattg gcttttgacc
     3121 gattgtaaat ctcagcatat tgttgtgcat cacaaacttt tacattgtct ttgctccacg
     3181 gttcctgtaa accccagaac atatcaaggt tggcctggaa cctgtctgtc tggccccttt
     3241 tggtggtaat aaggataacc ccgttacctc cggcagaacc atagattgca gcagaggctg
     3301 catctttcaa tatttccatc gattcgatat cggcagggtt tatgtttcct aacgaaccgc
     3361 caggcacacc gtcaacaatg accaatgggt taccaccgtt gatggtagcc aaaccacgaa
     3421 cacgaatctt aggctctgct ccgggctgtc ctgagttttg gtaaatctga accccggcag
     3481 ctttgccttg caatgcttca tcaacacggg ctgttggcat atttttcagg tcttttgaac
     3541 ttacagaagc aacagcgccg gtaaggtcac tcttcttttg cacaccataa ccaattacca
     3601 ctacttcatt gagctctttg acatcttcct gcatcactac attaatatca gcgctctctc
     3661 ctactgtcac ctcagaaggc ttatagccaa tgaatgtaaa agtcaacaca tctcccagag
     3721 agcatttgat tgtaaatttg ccatctacat ctgtaacgac tgctacattc gagcctttta
     3781 caatgacatt taccccagga atcccggttc ccgtagcatc ttttaccacc ccggaaacac
     3841 ttttattctg gccaaaaaca ggatatgccc acagcaatgt aacagccaac agcaaatacc
     3901 ttagcctcag ccaaaacaac tttataaaac ttttttgcat cataatagat ttgttagtta
     3961 gacatttatt tgtatgaaaa atatttgcaa tttgtaacgc gacaaatata gatggtagcc
     4021 caaatggcca tattgcacat ttgttttgat tgcttgaaca aatgttttgt ctttgacata
     4081 aatgttttca ggagataaaa tgattgtttt taacacattg ttgtccggta aaaacaggtc
     4141 gccgctatgc ggcttagaaa catttggtgt atttggttat cctacaaaca gttcgcagct
     4201 acgctgctat tatttagtcg cgtagcgacg tcctgtttgt agtaaaatta aacatcgtta
     4261 aaattaagcc ccagcggggc gaccaatttc taatcaacga tattactagt atttataagt
     4321 attttatttt accggacaac agtattttta ataaaaaaca gattcttaat ctcttcatta
     4381 gtaattcgta attcttcatt agtcattcgt aatttatcct aaatttgccc catgatttat
     4441 ccatcaaatt ttgaacagaa gattggcttt gacaggctgc gagcccgtct caagacattg
     4501 tgcatttctt cccttgggaa agactatgtt gacaacgttc atttcatcaa agactttgaa
     4561 cttttgcagc atttactatc tgtatgtaat gaaatgatag taatattaaa gacagaagaa
     4621 aacttccctg ccgaaaactt tattgattgt acctcatacc taaacaggct taagatagaa
     4681 ggcaccttta ttgagcctga agaaatgttc aaactgagcc tttcgcttac tacaataaaa
     4741 aagcttactc attttttcaa agaaaaagga gaagggaaat atcccattct tttcagcaaa
     4801 agtgctgaag taaaggtctt ttcgtttgtc caggaacgta tcgaccagat tgtagcccgc
     4861 aatggctctg tcaaagattc tgcctcgcct gcactgcaca agatacgcaa agaaatggca
     4921 gcgctggaaa ccgaaatatc ccgccgtatg gcacgcatca tggaacatgc acgccaacaa
     4981 ggctgggccg aagatggcgc atcacctgcc gtccgtgacg gaaggcttgt tattcccatt
     5041 gatgccactc acaaacgtaa gctgaaaggc ttcatttatg acgaatctgc caccggaaaa
     5101 acagtttaca ttgaaccggc cgagttagta gagctgaata atgatttgaa agaagccgaa
     5161 catgccgaaa gaagagaaat tattaaaatt cttattggtt ttgccaatga tatcaggcct
     5221 tatatagatg agctgcttat aggttatgat tttattggac ttatagactt caaccgggct
     5281 aaagccttat ttgccaacga aacacaaagc gtcatccctc cgctcaacaa tagacctgtg
     5341 ttggaatggt acaacgccgt tcaccctttg ctttactacc atctgaaaga agaaggcaaa
     5401 aaagtcgtac cacttaatat acgcctcaac gaaatgcacc gcatactggt tatctcaggg
     5461 ccaaatgccg gaggaaagtc agtttgcctg caaactgcag ctttattaca atacatgctg
     5521 caatgcggac tgcctattgc tgtcaaagaa accagcgaaa ctggcatttt cgaccaaata
     5581 tttattgaca taggcgatga acagtccatt gaaaacgacc tgagtaccta tagttcgcac
     5641 ctgctcaata tgaaacattt tgtcaaaaat gccaataaca agacactgtt tctgatagat
     5701 gaatttgggg ctggcacaga gccaatgctg ggaggggcta tagccgaaag catcctcgaa
     5761 cagcttaaca ctataggggc ttatggcatt gtaaccacac actacaccaa tcttaagcat
     5821 tatgcttcgc aagcccaagg aattgtaaac ggagcaatgc tttatgatac gcaacacctg
     5881 caacctcttt tccaattagt catcggcgaa cccggaagct cgtttgcttt tgaaattgcc
     5941 aaaaagattg gtatgcctga agttattctc aaggctgcta ccgaaaaagt gggcgaaaaa
     6001 catatcgact acgacaaaaa cctcaaagaa attatccgcg acaaatacta ctggcaacaa
     6061 aagcgaaacc aggtgaagga taccgccaaa aagcttgacg aaattaccag ccgatacacc
     6121 gcagaacttg aagacataaa aaagttacgc aaagaaactc tcaaaaaagc ccaggaagaa
     6181 gcccaggcta ttttggccgg cgtaaataaa caaatagaac acaccattaa agaaatcaga
     6241 gaaaaacagg ccgaaaaaga aattaccaaa cagctcagac aaaacctgga aaagaaaaaa
     6301 ctggaagttg atctggccct taatattgaa gacgaacgta ttaacaaaaa aattgaacaa
     6361 ctcaggcgca gagaagaaaa taaaaaacgc agagatgcag aaaaatcaaa tatcccagaa
     6421 attaaaaaac ctgaaaaacc ccaaaaagcc gaatttgaaa ctggcgacaa agtaaaaata
     6481 tttggacagg acaacattgg cgaagttatc agtgtcaatg gcaaaaatat catggtaggc
     6541 tttggcaaca tgattacaac ccttgatgaa aagcggcttg taaaaatcag ccacaacgaa
     6601 ttcaaaaaaa ttcaaaaatc taatcaatcg cacaccccat ttgcctctag aagctttgat
     6661 attattgaaa aaaagaaaaa cttcaaaatg cagctcgaca tcaggggcat gcgtgcagac
     6721 gaagccatgc aaaaagttgc cgactacatc gatgaggcca ttattgttga tgtttcagag
     6781 gtccgcatct tgcatggcaa aggaaatggc atcctccggc aattggtacg tgaatacctc
     6841 caaactgtag agcttgtcaa atcttttggc gacgaacatg ttgatatggg aggcgccggc
     6901 ataactttgg tgaggttcta atatagtctg attagtaaaa tcagaattct ggaataatct
     6961 ttgcttacat tttaaaactt tcagacattt gttgaatgtt aaaaatagat gaactttcat
     7021 atgttttcat tgttataaaa ataaacgact catgccaatc aacgtacccg ataagttacc
     7081 tgcaatagac attcttaaag aagagaacat ctttattatt gacgaaacca gggctgccga
     7141 gcaagatatc aggcctttga aaattggttt gctcaacctt atgcctatca aacaaaccac
     7201 cgaaacacaa attataagaa tgctttcaaa tactcccctt caggtagaac ttgagttgat
     7261 tagccttgaa agccatgttt caaaaaacac ctcgcaagaa tatcttgaga ctttttacaa
     7321 aaaatttaac gaaattggac aaagtaaatt tgacgggttt attatcaccg gtgcccctat
     7381 cgaacatctt gaatacgaac aagtaagata ttggcccgaa atccagaaaa taatggattg
     7441 gacgcaaaaa cacgtcacct ctaccctttt catttgctgg gccgcacaag ccgggttgta
     7501 tcacttttac aacatcccta agtacatgct tcctgcaaaa atgttcggag tttttgaaca
     7561 tcatgtcata aacccttcac tgcctctggt ccgaggcttt gacgatgtat ttatggcccc
     7621 acactcaaga cacaccgagg tacgcgaaga agatatccga aaagtaaaag aactggagat
     7681 tgtagccaaa tctaacgaag ccggcgtaca tattgctatg gctcaagaag gcaaacagat
     7741 atttgttaca ggacacttcg agtacgaccc tcatactctg agaactgaat atttcaggga
     7801 tgttgacaaa ggcctgacca tcgatttgcc taaaaactac tttaaagatg acgacccaaa
     7861 caatcatccc attgtaacct ggcgcagcca tgccaatttg tttttctcta actggctcaa
     7921 ctactacgtt taccagcaaa ccccttataa tattgacaaa ataggaagtt agttaatgac
     7981 gaattacaaa attactaatg actaatggaa agacacttca ttattcattc gtaatttgtc
     8041 atcagtcatt agtaattaat catcgtttta aagtgtttgc aaacctccgt caacccacac
     8101 tctttgcact tagggctgcg ggccagacat acataacggc catgcaggat gagccaatga
     8161 tgggccgcag gcaaaatatc ggctggcaaa tattttgtca attgcttttc acattccaag
     8221 ggattcttag cgtttactgt caagccaatg cgagctgcaa ccctgaacac atgcgtatca
     8281 accgccagca ctggcttatc aaacaataca gcacccagca catgggctgt tttgcgacca
     8341 actcccggca aacgctgcat ttgttgggta tctgtcggga gcttgccgcc aaactcagta
     8401 accagcatct gcgccatggc caaaagattc ttggccttgt tattagggta tgaaatgctc
     8461 ttgataagct gatatatatc atccacatta gcctttgaca atgccaatgg cgttgggtac
     8521 ttttcaaaaa aagcaggagt tgtcatattt acacgcttgt ctgtacactg cgccgaaagt
     8581 ataacagcta ctatcaattc atattcattt cgaaaatgca actcaggctc agctacaggc
     8641 atagttttct gaaaatattg ttgcactttt tcaaacaatt cttcacgctt cataaaccca
     8701 atagtttttt aacaaaacta aaccaatcta tagacaattt gtttctacat ttgccaggat
     8761 tttaacatct ggctaactac ttaaaataca aataatatga aaaatattaa atttattgtt
     8821 ttatctatga taagtgcagg aatactttcg tcatgcagcc atcagccttc aagctgtagc
     8881 actaatagtg cagactcttt tgcctggaaa accgagcagt ttgccgatat taaaatctta
     8941 cgttaccagg tccccgattt tgaaaagcta aacctaaacc agaaaatact ggtttactat
     9001 ttatcaaagg ctgctaatgc cggtcgcgat atcatctggg accaaaattg taaatataac
     9061 ctgctcatca ggagtacctt ggaaaatatt tacaaaactt attcaggcga cagaaattca
     9121 gccaattata agaagtttga agtttacctc aaacgaatct ggttttccaa tggaatccac
     9181 catcattatt ctacagacaa gattatgcct gagtttgaca aggcttattt tgccgaattg
     9241 gtaaaaaact ccgaccagaa aggatttaaa cttcctgaag gcaaaaatct tgagcaaaca
     9301 ctgaccatga ttgaacctgt aatgttcaat gccaatgtta tggctaaaaa agtatggctt
     9361 gacccttcaa aagacctgat agcaaactct gcaacacatt tttacgatgg aataaccgaa
     9421 aaagaagccg aaaacttcta tagcagcttg aaagacccta aagatgaaac tcctgtttcg
     9481 tatggtttga actctcaact aaccaaggtt aacggaaaaa tcactgaaaa agtatggaaa
     9541 gttggaggca tgtacagccc tgccattgaa aaaatctgct attacctcaa ccaggcaatt
     9601 ccttatgccg aaaacgaaaa acaaaaagta accattgaaa aacttatcag ctactacaaa
     9661 accggcaatt taaaaatttg ggacgattac aacattgaat gggtaaccga caccatttca
     9721 acagttgatt ttatcaatgg attcatcgag gtgtatggcg accctattgg cctcaaaggt
     9781 tcatgggaag ctatggtcaa cttcaaaaac ctggaagcta caaaacgcac agaaatattg
     9841 agcgccaatg cccaatggtt tgaagacaat tctccaatag acccacgatt tagaaaagcc
     9901 aaggtaaaag gcgtaagcgc taaagttatc acagctgttc aacttggagg cgacacccat
     9961 ccttctactg ctattggcat taatcttcct aatgccgact ggattagaaa agaacatggc
    10021 tcaaaatctg taaccttaga aaatattact tatgcctaca atcaggtttc tatgggcagc
    10081 ggtttttatg aagaatttat tgaaaaccct gcagatattg aactaataaa aaaatacaaa
    10141 gccctgagcg acaaccttca caccgacttg cacgaatgcc ttggccatgg ctcaggacaa
    10201 ttactaccag gtgtagccgg cgatgcttta aaaaactacc aatcaacatt ggaagaagcc
    10261 cgtgccgatt tgtttgcctt gtattatatg atggatcaaa aattaattga cctgggcata
    10321 ttgccatcat tagatgctgc taaagccgaa tatctttctc aaatgctaaa cggccttatg
    10381 acccagcttg ttcgtattga accgggcaaa accattgaag aagcccacat gcgcaaccgt
    10441 caacttattg caaaatggtg cttagaaaaa ggaaaagctg acaatgtttt ggaactttat
    10501 aaacataacg gcaaaaccta tttgcgtgta aatgattatc tgaaattgag aactctcttt
    10561 ggcaaattgt tggccgagat acaacgtatc aaatcagaag gcgattataa agcaggccgc
    10621 gacctggttg aaaactatgg ggttcaggtg gacaaagcaa ttcataccga agctttagaa
    10681 cgctacaaaa aattaaacct ggccccttat agcggcttca tcaacccaaa actttctctt
    10741 gtaaaacaag gtgaaaaaat cattgatgta aaaattgaat atcctgacaa ctttgcaaaa
    10801 cagatgctcg aatacagtaa cgaatttagt accctgcctg ttttaaacta aggcaaaaag
    10861 acaaaaggga taagacacaa ggcataggta accccattcc ttctgccttt atacttatcc
    10921 ctttttagtt ttgctttttc ccttttctct ttttgcctta tcccttttta ctttaatttt
    10981 ttatacattt gtcacataaa cttttatcat ggttttttta aaaggaatac gagaaaaaat
    11041 aggacacatg attttgcgca aaaaaatcag aatgcgcaaa agaagtaaaa aaatctgcaa
    11101 ttttgacaca gcccaaaata taggggtaat attcaaaaca gattctcaaa gcgactttga
    11161 gcttgtcaaa cgtttcttgc aatttctttc agaccacaac aacaaagtgt ttgcgctttg
    11221 ttttgtagac agcaaaaagg tgcctgattt ttacttattg cgcaaaggct tcaatttttt
    11281 ttcccgggca gaacttagtt ttttctttat tcccaagtct cagacaataa tcaatttctc
    11341 cgaacaacca tttgacatgc taatagacct gagtattgac gaaagtttcc ctgttcacta
    11401 tatcagcgct atttcaaatg ccaaactcaa attgggcaag caaaagaaaa actcggacca
    11461 tatcgacatt atgattgatg taagaaaaaa caattctgtt tcacagctca tcgagggtat
    11521 aaagcaatac gtccctgttt tatgccagta attcaaaagc taaaaaaact cccgtattta
    11581 ttggggcatt ctttttttta tccggcaaga tttatatttt tggacactta atttttaatt
    11641 atgaatactg cgaattcgct atttaaaggt accggggtgg ctattgttac gcctttcaga
    11701 aaagatgaca gtatcgactt tacagcattg tctaatttgg taaactacca aattgaaaat
    11761 aaagcagact acattgtagt acttggtact acaggagaat ctgtaaccct ttcaaatgat
    11821 gaaaaacatg ccgtgataaa taccgttatt gaagctgcca ataaacgcgt cccgctaatg
    11881 gtcggaattg gcggtaacaa cacccaggaa attgtaacca aaattaaatc atttgatttt
    11941 acaggtattg acggggtatt gtctgtctcg ccatattaca acaaaccaac tcaaacaggc
    12001 atttacgagc attacaaagc tattgcaaac ataagcccaa ttcctatcgt actttacaat
    12061 gtaccaggcc gtactggcat gaatgttact gccgaaacaa cattacgcct tgctaatgac
    12121 tttaaaaaca ttattgctgt caaagaagct tctggcaata tgcaacaagt aatggatatt
    12181 ttaaaaaata aacccgaaaa ttttagtgtt atatcgggtg atgatgcttt agcattcccg
    12241 ttggcctgtc ttggttgcga tggagttata tctgtcacag caaacgttgc caccaaagag
    12301 gtttctaata tgattcgctt tgctttagaa caaaaatatg accaggcaag aaagttgcat
    12361 taccaattgc ttgatttgac tactatgttc tttactgaag gtagccccgc cggcgttaaa
    12421 gctgcattgg atgttaaaaa actcacttct gcccatgtcc gcctgccatt ggttcctgta
    12481 tccaaaagct tgtacgacaa aattgcagaa aaaatgaaag aatttaaata aaaatccttt
    12541 gatttatatg aaaaaagaga atgtaaccca ttctcttttt ttatgtttta tcactaaaaa
    12601 ccaataaagc taaaagatat cttccaggct atacccttct ttcagcaaat tggccaaatc
    12661 taaagcacgt tgcatttgtc cttcactgac aaacatcaac gctttatttg cttctataat
    12721 atttatatga tgttgtttca tatacaaagc aagctctgtt gcttttttat aaccaataac
    12781 cggcaccaat gccgtactaa ttgaaggact gtatagcatt cgctttttcg cctcttctgt
    12841 agaaagctct aagccagtaa acaagttatc tgccaatgtt ttgtttatag aaatcaacgt
    12901 ctttatgcta tttatcattg catgcccaat agaaggaata taggcattca gctccaaaca
    12961 gcccaaagcc gaaagattgg taagcaacgc atcattagca tatacctgat gagccccgct
    13021 aatggcatat tctacaataa ccgggttgac ttttgccggc ataattgaac ttccggcctg
    13081 cttctgaggt atttttattt ccctgcctgc aatatctgaa gccagcaacc tcaaatcatt
    13141 ggtcattttc tcaatattca ctgcatgagc tttcaaagta gcagatactt caacaaaagc
    13201 atcaagattg gatgttgcat caggcatatt ttcagacctg aaaataggta agcccgacag
    13261 ggcacttaaa ttacgagccg attcgaatac aaaataccgt ggaacagcca aagcagtgcc
    13321 aattgcccca cctccaagat tgaccgtttt gatgcgctca taacacttag acactcgcca
    13381 ccagtcgcgc gacaaagctt cgttataagt actgaacaaa cggccaaagc ttgttgggac
    13441 ggcttcctgc atttgggtat agccaatccg taaatgctga tgagcctgtt tttctataaa
    13501 ttcaattttt gaccttagtt catttataga tttttcgagc acttcaagca aacgtataac
    13561 acaaaccttt aaagatgtag gtattacatc attggtagat tggtaaatat tagcatgctc
    13621 aaatggatca accagagaat aattgcctgg ggcagatccc aataactgta acgcccgatt
    13681 ggcaataatt tcatttacat tcatgttgat gctggtgcct gcgcccccct gcaaagcagg
    13741 tacaataaag tattcaaaat gcataccagc ctctacttcc tcagccgcct taatcaaagc
    13801 ttcaataact tcatcagaca tcaatgcaaa aggcaatgct ttatcgctgt atttctctga
    13861 tacagctttt ttatagttct tgtatgtgtg ataaacagcc aattttacta atcctaccga
    13921 gcaataccat tcttttgaaa aaggtgtatc gtccggaaag ttagtttttg cgcgtaatga
    13981 atgaattcca tataaaacat tatcaggaat ttcaagagtt ccaataaaat cggattctgt
    14041 gcgcataaag ttcatttaat actttgatta ctactcttcg tcttcatcag tatgagagcc
    14101 ttttgtatca agcccggcaa cacaaaccac tatctctcct ttgacagaag gccttgattt
    14161 cagctctgct atcacttcct ccaacgtccc acgaagagtt tcttcatgca acttcgatat
    14221 ttcgcgcgaa actgttactt ttctttgtgc accaaaaaat tgaacaaact gctccaacgc
    14281 ttttacaata cgcatgggcg attcataaaa aatcatcgtc cgtgtttcct gcgaaagcat
    14341 ggccaaccgt ttctgacgac ctttcttttg aggcaaaaaa ccttcaaaac aaaaacggtc
    14401 tgaaggtaaa cctgaaacta caagtgctgg gataagagca gttgccccag gcaagcattc
    14461 aacatgcaaa tcagccgcca aagcttcacg aaccaacaaa aaaccagggt cagaaataga
    14521 tggtgtccct gcatcagata ccaatgcaaa tacctcacca ccctgcatcc tgtttatcag
    14581 gttcgacaag gttttatgct cgttaaactt atgaaacgga accataggct tacttatacc
    14641 caaatgtttt aataaaactg acgactgacg ggtatcttca gcaacaacca catccacctc
    14701 ccttaaaaca ttgatagccc tcagggtaat gtcatcgaga ttgcctattg gcgtaggaac
    14761 tatatataaa ttcgttttca tcaactatgc attagcaaat ttccggttta tcagttgaag
    14821 caatttttgg gctgcatcct tttccaagtc aatatttatc tcagctttaa gaaataaaac
    14881 tgcctcttcc gcacttttca tttcttctat tttttgcaat gattcagcat aaagctttgc
    14941 atcatcacga aacaattctt ttataaaaag aaacctgtcg tttatttgga tactctgggt
    15001 caggcttgca atctcttttt gctgaaactt tgtagaaagg tcggtaacat ttaccttctg
    15061 agatactaca tcatgcaata aaggctcagg ttttgcaagt ttctcagcca gtatttgttt
    15121 gtgatgctgt tgtttttcat gagtttcttg aagctgttca acaatttttt ctttcactgc
    15181 taagggttca gtttcaacaa taagctcgtc ctctttaggt tgttcaacaa cttcattttt
    15241 ctgcatctta ggctctttta caataacatc ttcaagccct tgcttgattt taaccggctc
    15301 aacctttttt acagcctcct tgacaggccc ttcaacggcc ttatcaattt ttatctctaa
    15361 aacagcctca tacatctcgc gcaggttctt caatacaatg tctttttcta ttgaagaaat
    15421 aacatttttt tctccaatag aattaagcat ttcagctatt ttggaatgat attttttcag
    15481 cttatcaaca gcagatttat ccataaactt ctcaaattaa gtgaacttca gaaaagtcag
    15541 tcaacattct gttaacattt tacaaatata caaaaggcta aattttattc ggcctaaaat
    15601 attgtagcgc aatttgcttt acttttgcat caacagatgt tttttacaca tcgcatattt
    15661 atcatggact acagtttaaa acaaatcgca gaaataacag gaggcaagct cattggagac
    15721 ggttctgtta tgattaacca gcttatcacc gatagccgac aacccgttgc tgcttgcggt
    15781 tcgttattct ttgccattaa aggcccaaga aatgacggac atcaatatat ccatgacctt
    15841 attgccaaac gcaatgtaac aaactttatt gttgaaaaag aaatttctgt cgagcaaaaa
    15901 ataaaccaga taatcgttga caatactgtt aatgcattgc aaaaactggc agcataccac
    15961 cgaagctgct tcaattgccc ggtaattggt atcaccggaa gtaatggcaa aacgatagta
    16021 aaagaatggc tttaccagct tttacagtca aaatataaca ttgttcgcag ccctaaaagc
    16081 tacaattcgc aaaccggcgt gcctctgtct gtttggaaca tgaatgaaaa aacagaactg
    16141 gccatttttg aagccggcat ctctaaacaa ggcgaaatga gcaggcttca ggctatcatt
    16201 aaacccgaaa ttggcatatt tacaaacatt ggagaagccc accaggaaaa cttttattct
    16261 attcaaagaa aaataagaga aaaattaaat cttttccgcg atactaaagt actgctttac
    16321 tgtcttgacc atgaagcaat agccgaagaa atagaagatt gggcaaataa caatcaacaa
    16381 ttgatctgct ggtctgcaaa acaaaaagca gacctgcaaa tcacccgtca aatcagccaa
    16441 aacaatagca catctttgac cgggacatac aaagacattg aaatatcaat taatataccg
    16501 tttacagaca atgcttccat tgaaaatgca atcacatgct ggctgctggt tttgcatctt
    16561 ggaatcgaca aaagcattta ccagcctgaa tttgctgcat tgtcacctgt agcgcttcga
    16621 ttggaacaaa agaaaggtat taatggttgt tctattatca atgacagcta taactccgac
    16681 ttatattcat taggaatagc gcttgatttc ttaagccacc aacagcaact tcaaaaaacc
    16741 cttattctat ctgacattct acaatcaggc aggttagact ccgacttgta ccagcacgtt
    16801 gcaaacctta tagaaataaa gcaagttcaa cgccttattg gcattgggcc ttcaatcagc
    16861 tcacaagcct cattatttaa ggtaaaaaaa gaattttata ccagcaccga agagtttatc
    16921 agccattttc agccttcacg ttttcaaaac gaagctatac tgcttaaagg tgcacgaaac
    16981 tttgaatttg agcgtatttc ttcatttctg gaagaaaaaa cacaccgtac caccctcgaa
    17041 atcaacatga cagctatggt gcacaacctg aattatttca aaagcaagct gcatgcgggg
    17101 acgaaactca ttgccatggt aaaagctttc tcttacggta gtggcagcta cgaagtggct
    17161 aatatgctcg aatatcaaaa agttgattac cttgctgtag cctttgccga tgaaggtgtg
    17221 gctttgcgac aatcgggcat tagcatgccc atcatagtca tgaacccgga agcttctgcg
    17281 ttcaggttga tgattgatta cagattggaa ccagaaatat actcatttaa agaactccgt
    17341 caatttgccg atatgctaaa agcataccga atagacaact acccggtaca cctgaaagtt
    17401 gataccggta tgcaccgact tggcttcatg cctgaagaaa ttgactcttt atgtaacgag
    17461 ataaagaaaa ataagggcat taaagttgaa tctgtattct cacaccttgc aggagctgac
    17521 gaagaacaac atgacaatta tacccttgaa caaatagagc tgtttcagaa aatatgttct
    17581 aaaattcaaa actgtgttga atatcctgtt atgcggcaca tattaaattc atcggggatt
    17641 gaccgttttt cagaatatca attcgaaatg gtacgcttgg gtattggttt atatggtgta
    17701 attccttcta atcaggaaca attgcaatgg gtgagcacac tcaaaaccag cattcttcag
    17761 ataaaagagg tcgaaacagg taaaactatc ggctatggcc gaaaaggcaa ggctttgaaa
    17821 caaaccaaaa ttgcagttat cccaattggt tatgcagacg gatatagccg aatactaagc
    17881 aatggagtcg gacgggtttt cgtcaatggc tcctatgccc ctgttattgg aaacatttgt
    17941 atggacatgt gcatgattga tattaccggc attgatgcta acgaaggcga tgaagtggaa
    18001 atctttggca aacatgtcac cattctcgaa ttagcagaca aaatgcatac cataccttac
    18061 gaagtgctta caagcattgc cccaagagtg aagagagtgt atgtacaaga ataaatgact
    18121 gatggcaaat gacgaatgac aaattgaaaa ctaatttttt agattcaatt ttaatggcac
    18181 actcaccatc attttgcaaa tgatagtatt ccccctcctt taaaaggaga gctgtctccc
    18241 gcctcaggcg ggaggcaggg gtagtttact tgtaaactac ttagccttaa tccccataat
    18301 cgaaagcatc tttaccccat aacgcttacc taatattcgg tatccttcag cgttgaaatg
    18361 ggcattatcc tgcgaagggc atccggctga agatataaca tgggctgttg gaatcgtttc
    18421 tgggagctta ttaataatgg cattcatgct tgaacaacaa ctcccttcag ccgaaacaac
    18481 ttctccggct aaaagtggca ccgactctgc ctttaaagaa aggtcggcga gcatatcgtt
    18541 gtatattttc ttaacataag taggccattg ggcatctccg gtattggttt cgccctggtg
    18601 aagaaggata ccttttataa ccccatcctt tttcgccaat ttagccaagt ctataaggta
    18661 tttgtacgga ttgtactgat aatcttttac cttattgaca aaccagctct ctttgtaagt
    18721 agaatcataa tcttgataaa tatctttatc aaacaacctg atatcgcagc cgcctacagc
    18781 aacattaata ataccaacag tgatactttc tggcaggttg gccaccattg tcctgccaaa
    18841 gtagtcggca ggtgagagtt ttgagtggca ctgacaggaa ggaggaatgg cattgtacca
    18901 ggttgctttt gtcctattta aattagggca atcaagcgcc tgaaatacct tgaaacgact
    18961 atctacaata gaatcctgag cctcaatagc cccctgccct tccatattgg actgcccaaa
    19021 acaaatataa atgtaaaaat taggattttg ggcattaagg cccattccta acaaaacaac
    19081 gcaggcaaaa acaaatagtt ttgcttttaa attttttgat ttcattttgt tttcaatatt
    19141 agttgattat ggcctcaaaa ataaacatct attttttaat gaggtttata ttttttggaa
    19201 cagcatacaa aaacaaacta aagaaaagcg ccataatcac gcatccaatc atccgctcaa
    19261 gcgtatcatc agtgaagaat gaaaacaata ccacgcaaaa gctgaccaaa aacaagtaag
    19321 aactatattt ccgttctaaa ataccaggca aaacaaacga aagcaaaaat accaccagcc
    19381 caacaattcc gctgcctatc ataatggtaa gcaactggtt atgtggattc tgttttttat
    19441 cttcgcccaa atccgggcta tctttatcaa actgagcttg tatatcatca ttgatatccc
    19501 catagccggt tccgaaccat atatgctcag aaaaaacttt ccaaccattt ttcaaaccat
    19561 ttattctgat agttacagaa ccagccggaa tacctgttcg cctccactcg tcaaactccc
    19621 tgattaaatc atatatgcgg gcattaacac caacctcagc caacttataa ttataataac
    19681 ccgattcaat atttcgtaca tcgcggtcgg taagctgcga cataccagcc gaatctttgc
    19741 gcaacccttt tgaggtaaga taactgacaa gcgttacttt taccggcacc caaccagcat
    19801 tatcgaggct atcaaacgca acctggcttc gttttgccca ttgaatacgc aattctggct
    19861 cgcatatata aatattcctt aaatatccat taacacgaat agtatcattt acactatgct
    19921 catatttatt gccattcaat gtcaatgagt ctagttttgc tgtattttca atatgtattg
    19981 cccgatactc attcaaatta cttttgaaca aatatgctcc agttgcaata cttaataatc
    20041 caagaactac aaatgcaact cgaatataaa cactaatttt tctatacctg accaataaca
    20101 caaccagtaa agtcaaagcc aatgccaaaa tccctgtcaa tgattgtaaa ataccaagaa
    20161 atacaactaa ccaaactatt gatattatca tcaatacctg aaattttcta ttttgcgagt
    20221 gatatattgc atataacaaa aacaagatat caagtactat caacaaagca aaccttacag
    20281 actcaacaaa cactgcaatc tgacgcacat tcgcaatttt gtattcaatg acgtgaaaaa
    20341 aaattaataa tgaaaaaaat gtagcggcaa tattggccaa tgtataaacc cataacaata
    20401 ctttaagttg ttgccatgac aacttttcaa aagaaccaat aataataggg aacagaacaa
    20461 ttgatatttt gatgttcgta atcaaaaaaa tatttctctg aatattttca gcataaaaca
    20521 agcctgccaa aacaaataac gttgacccgg caaatatcca aatgcttttt ctttgcccaa
    20581 gggccttaac ccgttcttta aacccgggag ctacaaacca cgagaataca agcaacccct
    20641 cgcctatact aaccaatgct tttgacaagg gcaaacctgc caacaagaca actaatgaaa
    20701 aaaaataaat attttttctc agcacatcct ttacaacata ttgataatat gacatatata
    20761 atcttttaca ctttttacat cgtaaaaata gaacgaataa tcaaaaattt gttaaaaatt
    20821 aatatttttg aacaccttat accatgatat gcagcaaaaa gctacgacac tttgagtata
    20881 atgattaaaa ctaactaaac ttgaagcaaa gccaaaatat tttattaagc aggattgatg
    20941 cgattggaga tgttgtactt tcattgcccg ttgctggcat tttaaaaaac cttttcccgg
    21001 attgtaccat cttttttttg ggtcagacat atacaaaaga tgttattaaa acatgccaat
    21061 atattgataa tttcattgat tggaaccaaa taaaagacct aaaccctaaa actcaaattg
    21121 attttttcag aaaactcaac attgatgttg ccatacatat ttatccaaac tttttcattt
    21181 catggattct caagtctgcc ggcatcccta tcagaattgg gacaagcaga agatggtacc
    21241 atcagctttt ttgcaacaaa aaaatatctt ttagccgacg ctcttcaaac ctgcacgagg
    21301 taatgctcaa cctgaaacta cttattcctt tcgatctggg aaaaacaata aactacgaaa
    21361 ccctttcaat cataaatggc ataatacctc caactatacc agatgaatta aattcgctgc
    21421 tggacaaaaa ccgttttaac cttatcatcc atccaaaatc taaaggacat ggaaaagaat
    21481 ggggcattga aaattatgca aaactgttag gcctgctcga taattcaagg tttaattttt
    21541 ttgtaacagg aacactggat gaaaaaaaag aaatccagca agaaatacta aacaaatacc
    21601 cacaagcgat agacttagtt ggcaaactct cattggacga gcttattggc tttatttcaa
    21661 gggcagacgg gttagtagct tccggaaccg ggcctctgca tttagctgct gctaccggaa
    21721 ttcatgccat tgggcttttc cctccaataa gaccggtctt ttcgagaagg tgggcacctg
    21781 ttggggtaaa tgcaaaagct tttgaagcaa aaaaaatatg cgtaaaatgc aaaaaaaacg
    21841 ctaaatgcga ttgccttagg ctttttaaac cagaaattgt tgcagaatac cttaacactc
    21901 tacataaacc ttcgcagaaa aaacaactgg aagccaatat ttttaaacct tcagcacttg
    21961 attgggagca attcaggata caacaggccc aaaaatttga aaattttgta agtgacaaaa
    22021 aaactaaagt atttacttca acaaatcaga ttttcaaaaa gaatagaatt attgtttcgg
    22081 tagataaaat aaacaagcct aattgcggac ttggaagagt agctattgat tttagcaaag
    22141 cgttgattaa tgaaaaaaaa gaaaactgtt acttttcgtt catccttacc ggaaacaatt
    22201 cgtacgatta ctttagggac caggacataa aaaggtttaa gttcatccac cgctttttca
    22261 aggccgattt tggagagtat gacgtagccc actatttaca ccaaaccccg tcattcaaaa
    22321 tcaagggagc caaaaaaaca atattgacca tccatgattt gaatttttta tataccaaaa
    22381 gcaacattaa agctaacaga tatttaaagc atatccaacg aaatgttgat aaagcagatg
    22441 ctatagtttt tatttcaaat tttaccaaag aaatttgttt tcaacacctt caaatctcgc
    22501 ccgaaaaaat cacaagagtt atttacaacg gagtaaacct gcctaaaact atcccgataa
    22561 aaccgccatt cattaatggg acaacattta ttttttctat tggacaattt ctggctaaaa
    22621 aaaattttca caccctaatt ccctttatca aacttttccc atctaatatt caacttgtca
    22681 ttgccggaga aaaagacaca tcgtatggca actacgtccg aaaactaatc agtgactaca
    22741 accttcaaaa ccaggtaatc ctccccggtt ctgtttcgga ggctgagaaa ctttatttat
    22801 atcaaaattg cgaagcattt gttttcccct ctttagccga aggctttgga ctacctgtta
    22861 tagaagcaat gcgggcagaa aaaccagtat tttgctctga caaaaccagc ttaaaagaaa
    22921 ttggcaatgg ccatgttttt ttctgggaat cgtttgaccc gcaacaaatg tttatgacct
    22981 tccagaatgg catgaaatcc tttgacgaag ctaaaaaaaa atcggcatac gaatatagca
    23041 tgaagtttac ctgggaagaa aatgctcgtc aatacctttc tctataccat ttatttactg
    23101 aaaatgaata agatattcat ttattgcccg ggtaatataa taagtggggg aacaaacagt
    23161 ttgcacattt tgtgcaaagc tttagaaaaa aatggctttg aagcagggat gtactatatc
    23221 aacccggttg aaaagattat taacagtgat attgtccgtt ctttccaaac cacttcattt
    23281 ggccctccaa aagacctcga acaaaatatt attattgtcc ccgaaacaat ggtggggtat
    23341 ctaaaacaat ttaacaatgc ccaaaagata gtttattggt tgtcctctgt ttactttttt
    23401 aaaaaccctc cttttaaatt tccattcaat ataaaaattt tccgaaaacc catctatctg
    23461 gattggtaca cctatcaatc acaaagcttg tcaagccgtt tgcgggtcag acttctaaaa
    23521 tgggccaaaa aagacagtca aatatggcaa aattcatacc accatattac aaactctttt
    23581 tttatctcag actttctttc atttttgggt atcccgcaaa catttgtctt acacaatcct
    23641 gtaagagatg agttttacca gccccttgca aacacaccca aacaaaaatt aatacttgtt
    23701 ggccccaaaa ccccaaaaaa tatattatat attataaaga aaagatttcc aggatttcag
    23761 gtattacaag caaaaaaaat ccctcccgaa gaaatgttcc tgctttatca aaaaagcatt
    23821 ttatttattg agcttgggca ctcaaaccgc gaccgatgta tcagggaagc cgccctgaga
    23881 ggttgtataa ttttagttag caactcggga ggcaataaaa accaacacga cacccctata
    23941 gctgattatt acaaaataaa tacaagcaaa aaatacaata atttactcct taataagatc
    24001 gacgagataa taaaaaatta tgacaaacat cttgcaaact ttgaacattt caggactttc
    24061 cttttagaag aaaaaaaaca atttgagaaa agggctctat tgatttttga gcaaataaca
    24121 agcaaaaatt aaaatttctt tttagcgcaa acacccaaag caaagctatt tcccaaaaag
    24181 gctcctttat cccaaccaac acaaagctgg acatgcaagt ctttcaatga aggaatatct
    24241 ctgccaactt taaacatcat tgaatgttgg cctttttccc ctatcggaac gctatatgta
    24301 cctttattaa tcgaataaga atattttaat tcacactgca aatgattata ttcatataaa
    24361 cctgcaaaat aaaatgccaa taccctgtta ttggtgacaa tattactatc tttttcatta
    24421 atagccggag atgttataaa tggcgtacca atggtattac ctttataagt ccatgattga
    24481 taaatataat tattaaaata attgtcattg ccagctttag ggttccaaaa cggaataggg
    24541 acatcagtca cccaaaccaa ttcaataggg ccactttgat aggtcgtttt tgtaaactca
    24601 agtacgacct cttttaaatg agctgtttca tcattattct taaatgtcag gccatataat
    24661 ccgtctcctt gaggtgagat aaatttacta ccagatttat cttcaaagat tgtttgataa
    24721 tacaacccaa cagtcttgtt tttaaagtca taatcaagcc catattgtct tgaccccatg
    24781 tgattgccaa cctggttaaa agactcgctt tgtacaacat ttgcccctgg agggtttttg
    24841 gcaaaaaata aatatacaaa gtctttcaac gaactgggtt gcctgcctaa tgttgaagaa
    24901 acgcctcccc actgtagagc atgatggtac ccataattga aatgcaatgg cttctttcct
    24961 ccaaacctga tattaacata cttataatga atatatgaat ctctaaccca tcggttttca
    25021 ttctcagccc atccatgcgt aacctcgcct tttatttcaa cataaccttt ggtaaaaggc
    25081 acaaaagtat atttatctat gccaagcgaa actttaggat atggtctggc attagaagaa
    25141 aacaaaactg aaccactcga caattcggat gtctggttgc catatacctg tctataaagc
    25201 ccacctttga atataataca aaaagcccga aaagttgcat aaagttggtt gaactgcaaa
    25261 ttattacttt tatccctgat tccatacaaa tccatgccat aattaacctc aaatttcttt
    25321 tccctgtttt cttctgtacc catcaaaaca cgggcataca aacaattttt attattgtta
    25381 aataccccaa attgattact gtacaaccaa aaaggggcat tttctccatt tgacacaaaa
    25441 ccgccagttt caccatatac aaatggtttc tcaatcattt gcccaaaaac tattatactt
    25501 tgagaaagaa aaagtagaaa aaagaaaatg tttttcatcc gtaaataact ttttttaata
    25561 aggtcggatg aaccccgaaa ggctctccga agaaaaactc gtcccgatta gtcggtattc
    25621 atctttgttt gtgtttaacg aatcatgctc gtttcatcat aataaatttt attatttagg
    25681 gaatgaccgc ttgctccaac aaataaatat cattgtattt ttatttgcca acaatcatga
    25741 gagtttcatc aacccaaacc catcaaggca caaaagtatg gtattttatc ttatcgcttt
    25801 cctttttaat tttttttaat ttcatttttt tcttaacttg catctataac cccaaacttc
    25861 aaaaaatatg aaaaaatcac tattgctact ggcattagcc ggtatttatt gtattgtatc
    25921 aggccaatac tcttttatcc aggatttcaa aaatggcacg cagccagaaa caggctgggt
    25981 aatacatgtt ggaggcacat acaactcaga gcctaactcg ggaaaagcca tcccttcgtt
    26041 aatgttttcg gccaacaacc aatatataga aactccatca tttaaagggc aacacagctt
    26101 atccttctgg atgaaaggaa ataaaacaga ctctgcatca acaatgcaag tccttatttt
    26161 ttgtaataac aattggtacc ttgctcacaa aatatcgcaa atctcccaaa caggtgctaa
    26221 ttattccttt atgttaccag atacagcaga aaagttgaag tttgaatata aaaaaagcgc
    26281 cgggaatgta gccttagatg acatagtatt attcgccaaa aacttatttc cagaaatcac
    26341 ctatatcagg cttacagaaa ataatgacgt tgcagcttta attaatatca aaacaaacaa
    26401 catcggaaaa ataacctatt tggtaacatc gaaagatgaa ccctcaccca atgcggcaca
    26461 aatagcagac acctctctgt acagcggaaa aatattagat tatggaaaaa cagatattac
    26521 aaaaccagat tcgacaagta tagtactaca atgtttacca tcaaacagca atttgaaagc
    26581 tttcttcatt gttgcagatt taaaagactc gttcagcatt gaacaacagc cggtgaaact
    26641 gggaataaac acaccagaac gcaacagata cccatttttt aaaggcattt ataaaggtga
    26701 tgccagcgcc gaaaaagcta tacaaatatt taaccccact aaagaaacta taaaccttac
    26761 caattataga attttagtag ctactaatgg caaaggctgg ggctttgatt actattttga
    26821 aaatggcaaa agtataaaac caggctctac ttacctcata gtaaatgctg gagctgaatt
    26881 tatagacatt gataaagctg acgaaatcac tacttctgtt ttatgcaaat tcagcggaaa
    26941 tgacgcattg gccttgcaaa aaaaatttga caattcaaat gcctggtata ttattgatat
    27001 atttggcaac ccatattcaa gcgcaaactt caatatagca ggtaaaaaat cggcggcaaa
    27061 taaatacaat gccatcagaa aagattctgt tgatactgga aatgtaaact ggtctgtttc
    27121 tgcaggaaat actgaaggag acagtgaatg ggaactcatt gcccttagtg attttggatt
    27181 attgcccatt gttttgcccc catatatgcc aaaactaagc ttcatagata tctcaattga
    27241 tggaattgca gcccaatcaa ttgattccat taactacaca ataactttta gcacaaacgg
    27301 gactgtaaat gatttaaaac taaactatcg acttgactct gcagcattga tagagccatt
    27361 gcccgattct atcagagatt attcgcagcc cgtcatttac agcatatttt caaaagaaag
    27421 tgaagacact gtaaaatgga agataatagt aaatggaaac aaaaatatca ttactaatat
    27481 taccaatgaa aaacctttta tttattataa tcaacaaaac aaaacgattg ttatcaaaca
    27541 aacttctaat gaaaatttca gctatgccat tatttacaat agctcaggac aggtaataaa
    27601 aaaagcaaaa ttaaacttcg aaaataattt tatcaatata cccaagctcc cttctggttt
    27661 atatgttatt aagctattaa aaaacaatgc aaaatacagc aaatcaacaa ctattaacat
    27721 ttttaggcga taagaaattt ttatcttaaa aaatcttgta tatatgttaa gatttttatt
    27781 gttttgcttt ttgtattttc aaaagaatta taattttaca ttaattgact ttttaccaat
    27841 taaaaggtag ttaaatttgc taaacaaaag aatactaata aattaaacat ccgatatgaa
    27901 aaaaattatt tacctgggaa taatagttgt tattgcctta tgtgtagcca gttgcaaaag
    27961 tagtaaaaaa tgccctgctt atagcttcca aaaagccaat acagaacagg ttgtaaaagc
    28021 ttaatattac agtcgtttgc aaggaagttg ttttctggat aatatcttga aaacaacttt
    28081 ttttatgctt gtttcaaaaa atttacaaca gcgttagcaa cattttgtcc gtctatagct
    28141 gatgaggtta ttcctccggc ataaccagcg ccctcgccac aagggaacaa gccttttatc
    28201 tgaacatgct gcatgttttc ctgattacgg ggaatacgaa ccgaagacga tgtacgggat
    28261 tcaactccaa gaactacagc ctcagcagta acaaagccat gcataagatt gtcaaaacgt
    28321 ttaaagccct cctttaatga acttccaaca aaaccaggaa gccacatatg caccggagag
    28381 gatacacatc ccgggaaata tgatatttgg ggtaaagatc ctgaaatttt acctttaata
    28441 aaatcagtca accgctgagc tggtgcagtt tgctgccttc ctccgttttg ccaggccata
    28501 ttttctaatt gttcctggaa atacaaacct gctaaaggcc catatttggc aaactccttg
    28561 aggtcttcaa ggcgaacttc tgtaacaatg ccagaattag cataaggtga gttacgtttt
    28621 gaaggtgaca tgccatttac aaccacctgg gtgtttgttg tggctgatgg tacaataaac
    28681 cctccggggc acatgcaaaa cgaaaataca cccctgccat tcgcctggca tactaatgaa
    28741 taagaagcag caggaaggtt ttcacctcgc ttcaacccat gatactggat attgtcaata
    28801 agctcctgca gatgctctac cctaacccct acagcaaaag gctttgcttc caatgcaata
    28861 tgttgttcgt gcaacatctg gtaaacatcc ctggccgaat ggcctgtagc caacactaca
    28921 gcctgcgcct cgactttgtc tccattatgc aactgaacac cttttatttc ttcgtcttca
    28981 ataagtagtt gtgttatttt tcgttcaaaa taaaactctc cgccagctgc tattatcgtt
    29041 tgacgaatgt ttttgatgat ttcaggcaac ttatctgttc ccagatgagg atgggcctcg
    29101 tacataatgc tttctggagc cccatgccga acaaacacgt ccaggacgtc tttaatattg
    29161 ccgcgtttag aagaacgggt gtaaagcttg ccatcagaaa aagtaccagc gccaccttcg
    29221 ccaaagcaat agtttgactc agcattaacc tcctcgtttc ggctaatcag ggctatatct
    29281 tttttgcgtt cgctaacctc ttttcccctt tcgaaaatca aaggtcgaaa accatgttct
    29341 ataagcttta aagctgcaaa taacccggca gggccagtac caacaataat tatcggtttg
    29401 gcatgatcaa cttttttata aattttaggc tgtatatcct ctttaggcgg ctgttcatca
    29461 ataaaaacaa ggagtgtata atttaccaaa attggcctgc gacgggcatc caacgatttt
    29521 ttttcaattt taatatgctt aatccgctct atattgatct gaagctgttc tgctacgaca
    29581 gggcataaac aattaaagct atcatcagtt ttttgggcat ttaattttaa gctaacctgc
    29641 tgctgcatgg gctattcaaa atgaaaagaa atcatcagta tattggaatt gagctttcgg
    29701 atagagttca cgtaaacagc attttccttg atattctcat ttgtcttgtg attgagaata
    29761 tctgtcaagc ccaaagagag ttttatttct gtagaaaatt tgaaatattc taaataaaaa
    29821 tccaccccaa aacccatttc taaatagaaa tccagaggtt ttaacaaaac ataaggatgc
    29881 ttatcatcaa attttgattt tgaagctaag tcgtaacgca cgcttccacc ggcaataaaa
    29941 taaggcctgg tattgttcaa tcgcatagcc ttgtatttga taagtattgg aaggtcaagc
    30001 atgctggatt caagcttaac ttcttgcatc tttgtatcaa ccttatatac tgatttacct
    30061 gaaaaatatt gaagtgtacg ttgtccaaaa ttaaaacttg gcaggcagcg caaattgaaa
    30121 tattcgccta acctcaaatc agaaatcatt cctacttgaa accccaagcc taagccaagc
    30181 tgatcagcat aataattttc atttagccca ttgtaattgc ggccaaaagt aaaatccatc
    30241 acattgaggc ccatagtata accaaagtgc agaggtttgt catcgtactc ggggttatgt
    30301 ttatatttgc ttttctgccc ccatcctaaa atctgacaac aaaccacaag aatgaaaaat
    30361 aatatgtatt ttttataagc ttgcattcag tttacaacat attaaatttt atcgccagta
    30421 taaaggttca ctatgccaaa agtcatgctt ttacaagtat tttgaacaaa cccggctttt
    30481 gtcagatgtt caagaaaagc tttgccatca gggaaactct ccaccgactc aggcaaatac
    30541 gtgtaagcag ccctgtcttt cgaaataaaa cgtccaataa acggcaatat ttttcggaag
    30601 taaaaataat agatttgttt gaccgggaaa tattttggct ttgaaaattc caacactacc
    30661 acttttcctc cgggtttaag caccctgtac atctccgaca agcctttatc aaggttctcg
    30721 aagtttcgta caccaaaagc aacagtaata gcatcaaaat gagcatcagg gaactgaata
    30781 ccctcagaat ctccttgttg caactttatt ctttgagcaa agcctttttt ttcaatcttt
    30841 tcccggccaa tactcaacat attttcagac aaatcaatac ctataatgtc aagctttgtc
    30901 cttttagccg ttgtaatagc caaatcgcca gtacctgtag caacatcgag cactataccg
    30961 ctttttatat gcttaatttt tgaaatagcc tttctgcgcc agaaattgtc aatacccatc
    31021 gaaagaaaat ggtttagaaa gtcatagcga aatgcaatgt tgtcaaacat ctgccttacc
    31081 tggctttttt tcgaaccttg ggtatcttta taaggtatta ccatgcattt acggttataa
    31141 aatattcagc agttgttctt taatcttctc cagctcatct ttcatcatca ccacaatgcg
    31201 ctgtatgtcg gtatcattgg ctttagagcc aatggtattt atctcgcgcc ccatttcctg
    31261 ggcaataaag cccagcttac ggccagctgc gctttcgttg gtaatggtct ggataaaaaa
    31321 ctgacaatgg ttgtgcaaac ggactttttc ttcggtaata tctatctttt cgaggaaata
    31381 aatgatttct tgctcaaaac ggttagcatc aatacttgca gccggaataa actcattgag
    31441 gtttttgcta agccgttgtt ttatcttgtc aatgcgctgt ttttcaaact gttcaatttt
    31501 tttaatagcc tcatcaatag tatttacgcg ttgtttaaca tcatgctcca aggcagcacc
    31561 ttcctgacgg cggaattgga taaaatcagc caaagcctca ttaataagtt tggtcaggct
    31621 tacccactct tcttcaatca ggccctgggc ttcgtttttt aacacctcgg gaagcctgat
    31681 aacagaagta tatagctgtt cacccacagt aacacccaat tcctcagcca aatccttaag
    31741 ttggcgataa taatctttga caacatcagc atttatggcg taatttgaag tagctacact
    31801 ttgttcaacc gttacagaca ggtctatctt acctctttca atagcgcgcc ctacaatatt
    31861 gcgaatttca gcctcttttt cgcgataaaa caaaggaagc ttaaggttta tatcaagctg
    31921 tttgctgttg accgaacgta tttcaattgt tacgttttta ttggcaaaaa ctccggtagc
    31981 tttgccaaaa cctgtcatgg ataattgcat gttttattat ttagtgttat ggggtttggg
    32041 gtcaggagtc aggatgtttc atatttcacc ccccagactc ttatccccag cctccttttt
    32101 aatccagtct tactttttca tttctataat cagattcaac aagcccgtcg cgcaacctga
    32161 cgatgcgatg agcatgcagg gcaatgtctt cctcatgtgt aacaagtatt atcgtatttc
    32221 ccctttgatg aatatcattg aaaagcctca tgatatcaat agaagtcttg gtatcaaggt
    32281 ttccggtagg ttcatcagca aggataatgg ccggattgtt tatcaaagcc cttgccacag
    32341 ccacacgctg tctttgacca ccagataatt cgttaggttt atggtgcata cgctcggtca
    32401 accccaccat gtcaataatc tgtttagctt tttcaatcct cttttccttt tcgacaccgg
    32461 cataaacaag tggcaacatt acattttcca aagcattgta tcgtgccaac aaattaaatg
    32521 tttggaaaac aaagccaatt tcgcggttac gaatggtagc caactcgtta tcattgagct
    32581 tgctgacact cgtaccattt aaaatataat ccccagaagt tggtacatcc agacaaccca
    32641 gaatgttcat aagtgttgat tttcctgagc ctgaaggccc catgatagca acatactcgt
    32701 ttttattaat ctcgatattt accccctgta atgcaggaac aacaatcttt cctacataat
    32761 aatctttgcg taaatcttta atactaataa tactttccat tgtattgggg atttttaggt
    32821 actattattt gtacttgttc attttatcgt caatagtaac agccttttgc gatgaagact
    32881 ggattttaag gtaacacatc atgtcttcag tacccgcggc ataacaggct gcatgtacct
    32941 tttctaccaa tgtcatcact tcggcatagg tgccttctac aaccgtttca aaagggcaaa
    33001 cctgatattt caggccggag gttttgataa tttcaatagc tttgtcaaca atatcatacg
    33061 ggtgtttgtc tttgctgcgt ggcaacacct gaatagcagc attaatctgt tggttcataa
    33121 tttatctgtt atatcttctt ttttgttggc cacttggagt atttcatcaa aagaaagtat
    33181 gcgtacatta gtaacaatgc aattttcgtc aacaaagtta ccgaatatat ttattttatt
    33241 gataacgtcg cgcataaaat gatgtattgg gctaacaatt ggtattagaa ggattatttt
    33301 tgccaaaaac ctttcaaact gaatatcttc taattctgcg tcataaactg gcaatccaag
    33361 ttctacttca tttttaatag ttttttgctg ccaatcagta aaatcaagac cttctcgatt
    33421 gaaaaaagca atgaaatacc tgagcttccg atctcttccg cccaggccgg tacaaagaaa
    33481 tatctgtggt tgttcgagca aatcggactg aatgtacatt tgggcttcct gtaatgccat
    33541 taatgaccat tgatataata caggatgctt ttgggataca aacttttcga taatacgaaa
    33601 tgattttaca tctttgctat gggccaactg aatgagaaga atctttttga tttcctcatc
    33661 tgcactttca tcatcaagca attcgacatt taattcacct tcaggcaaag acaatttctt
    33721 gttcctgtat ttttgggaca ggtcaaagta ctttacctgc aggtcaacat ctatcctttc
    33781 gttgataata ttcacttctc ctttaaaatc aacaagcagt tttcgtagat cttccaaagg
    33841 atctgttaat gcacccgttt cttccatgaa ttttttttag caaaaataag ctttaaaaaa
    33901 aactaccagg tatttggaaa gaaaaagatt tctatcattt ctatgccaca gctttcttgt
    33961 atttcctgac agctaaagtc aacatggcaa cagcataaat gcccatacag gatatttcga
    34021 acaaaaggtc tttaaaacct gaacctttaa gcagtatcat gcggataacc ctcataaaat
    34081 aatacgccgg attaagatga tttaccattt gtgcccaatt gggcatggtt tcgacggaag
    34141 taaaaatacc actcatcatg atacaaataa tcattacgaa aaacataata aacatagctt
    34201 gttgctgggt ttcagtaatg gtagccacga aaagaccaat acttaatact atcaccagat
    34261 aaatagctgc aatggcaaat aataaaaata agctaccctc aataggtatg tcaaaaatca
    34321 attttccaat ggtcaatcct acaccaagct caataagggc aagaatccaa aacggaataa
    34381 gctttccggc tataaactgg tattttttga caggggtaac attgatttgc tcgatggtac
    34441 caatttcttt ttccctgaca aggtttaaac ccgctaaaaa catacctaca agcgtaacca
    34501 aaaccaccaa aaccccgggt accataaaac atttataatt caactcagga ttgtaccaaa
    34561 aacggtgttg tatattcagt tgtgccggag caacattagt accagcaaca cctatagata
    34621 ctggtcggtt gtaccttccg ctttgaataa gctctgcctg ataatcggcc atgatagcct
    34681 gaatgtacga actggcaata ccggccacca taccgttgat agcattaaag gtagcctgaa
    34741 cttttgccga tgactcgttt aaaagctgtt tctcaaaatc gcggttgatg cacaaaacca
    34801 catctgcctt gccatcttgc ataagctgtt gcgcctggat agcagaagac atataattgt
    34861 caataacgaa gaatttcgaa gccagaaaat gcgaagccaa acgccttgaa gccattgaga
    34921 ggtcattgtc cacaatcacc atccggatgc ttttcatttc catggttgca gcattcacca
    34981 aaattaaaag ttgcaccaac ggaatgacaa atatgatagg caacatcatt ttgttgcgaa
    35041 aaatctgggc aaactctttt ttcaggaata tcaatattat acgcataaca gcagttttta
    35101 cctcctaatt ttaaactttt taatagccag caggataaaa aacaaattca tcgacagcag
    35161 gatcaacgtc tctttccata cctctttcag gccatttccc atcaacataa tatgtttcag
    35221 tatgataata aagtactttg gaggcattat attacaaaac cattgtaaaa tgagcggcat
    35281 attttctata ggaaaaataa aaccagataa caagatggtt ggaagcatca atcccaccgc
    35341 agaaacaaac atagcaacca tttggctatc ggctattgtt gaaataagta tccctaatga
    35401 taaagccagg gaaataaaca gaaaacattc gaaaaatagg agaaacaagc tcccggcaac
    35461 tggaaggcca aacacagacc atcctaatac aacaattaca aatgcattga taaatgaaag
    35521 taatatatat ggagtaactt tacccaaaag gatttcaaca ggcctcacag gcgagaccag
    35581 caatacatcc atgctcccgt attctttttc tcttacaatt gagacagaag tcatcaaagc
    35641 agagataagc atcaggataa gtgccataat acccggaaca aaaaaataca ctcctttaag
    35701 ctcaggatta tacgccatgc gaacctcagg aactatttgc aacatcccgg acttttgaac
    35761 atttttatca tttatgtacc cccgaattac agcagtggca taagaaaccg aaagattggc
    35821 aaaattggca tcagaagcat cagcaataat ctgcacattg gctttgcgat tgcgttgcag
    35881 gttatagtca aactgctgtt caaacactat agcaagcttt gcagcccccg atttaaaaac
    35941 actttcaagc tgaccataat tactaatccg ctgtttaact acaaaataac atgaagactc
    36001 aagcctgtca acaatctggt tagtagtgta atcattggat tgatccaaca ctgcaattcc
    36061 aatagtcttg atttcgttgg taataacaaa gccaaaaagc agcataagag caaccggaat
    36121 accaaacaaa atgagcattg tacgcttatc gcgcaaaatg tggtgaaact ctttctttac
    36181 cagacttaaa tatgtcttca taatgctttt tctactcaaa tactttactc tctacgaatt
    36241 caaaatcgag ctaacatcac tcatcgtcaa ctcattgaaa ttgttgatta ttttatcagc
    36301 ctttaccagt ttatcaggcg tgaaagaggt tgtaatagcc acaaccttca ttcctgcagc
    36361 tttggcagct tctataccta ataccgaatc ttcaataaca aggcagttct taggttcaat
    36421 gcccagtaat tttgaagact tgagataaat ttcaggatcg ggcttgccat gtgtcacctg
    36481 cgattcgtcc accaaaatat cgaagaagtt cctgatatcc agcttgtcga gaacaaagtc
    36541 aagattttca gtaggtgcag aggttccaac tgccaaacga taattgtttt tgcgcaattc
    36601 aaacaacaag ttcatcaatc catcaggagc agaaatactt gacgcaaaaa cctctctgta
    36661 aagcgcttct ttttcaatag ctatctcgtt aacccttttt gcatcattta taccaagtaa
    36721 cttttcgaca aacactttgt ttgtcccgcc aaatatatcc cttaccaaag gagccatctc
    36781 gggacgccca tatttttggc acatcatctg ccatgtttga tcgtgaacct tactgttatc
    36841 gaccaacacc ccatccatat caaaaattac tgctttcata ttgagaggtt agtgtttagg
    36901 ggtttgtaaa tagtcaataa taactctttc ttcatatttg ttgttgtttg tgttttttta
    36961 ttcgtaatta gtaattaaca ttccttgcta ctttaaaaaa taactcatca atagaattga
    37021 cctggtgctt ttctttcaaa tctttgggtt tgcccagttc aatgatttta ccctcggaca
    37081 taatggaaac cctgttacaa tactcggctt catccatata atgcgttgta acaaagaccg
    37141 taatgcccat ttcggccgtt tcgtttatca aatcccagaa ctgacggcgt gttatcgggt
    37201 caacacctcc tgtaggctcg tccaaaaaaa caacttttgg tttgtgcaac actgcaatag
    37261 aaaaagctat cttttgtttc cagcctaatg gtatgtcttt tatcagcttt ttctgcatat
    37321 cctgcatacc aaacttctca atcagctcat tggtacgttg ttcaattgtt tttttatcca
    37381 aaccataaat tccaccaaaa aaacggatgt tctccatcat gttcaaatca tcataaagcg
    37441 agaacttttg gctcatatag ccaatgcttt gctttatctt ttcgctttgt ttataaacat
    37501 cataacctgc aacataagct tcacctgacg ttggagaaag caatccgcat aaaatacgta
    37561 ttgcagtggt ctttcctgcc ccattagccc caagaaaccc aaatatctcg cctttttcaa
    37621 cagaaaaagt caggttatca ttggcaacaa acttcccaaa ttttttggtc atgtttttta
    37681 cccatattac agtactatca gccttatcta tatcctgaaa cctttccatt ttctattgaa
    37741 ccattaattt cataaaacaa tcttcaacag ttgggtttat tggctttatt tcaacattag
    37801 aaaaaccagt atttttgagc tctgtcacaa cattatcaat aaccccggta ttcagcgtaa
    37861 catgcacttc ctgcccaaac aaatgaacat ttttgcaata gtttgaatgc ttgaggtatt
    37921 ccttcatttt atagacttta tctgaagtta cagcatatag cacttcatta aaagaattga
    37981 ctacagcctg aggcgtatca atctgaagaa tttttccaga ttgcataagg gctacccggt
    38041 cacataaagc ggcttcgtcc atatatggtg ttgataccag gatagatatg ccttttttct
    38101 gaagattctt gagcattccc cagaactcct ttcgcgaaac agcgtcaacg ccagtagttg
    38161 gctcatccaa tataagcaac ttcggtttat gtatcagggc acaagacaat gccaacttct
    38221 gtttcatacc tccagaaagt tttcccgctg gcctatcttt aaaaggctct atctgcgaat
    38281 agatgtcact tacaagatgg taattttcct gaacagtagt tccaaaaagc gctgcaaaga
    38341 actcaatatt ttcttctatg cttaaatcgg gataaagcga gaacttgccc ggcatgtacc
    38401 caatatgttt gcgcaaagct ttgaaattgg cattcacttc cattccacat acctctgcaa
    38461 ttccactatc cgggaccagc agcgttgcaa caatttgcat aatggttgtt ttaccagccc
    38521 catctggccc gataagtccg aatatctcat tctcgttcac ttcaaacgaa atatcctcaa
    38581 tcgccttggt tgtgccgtag gtcttgcaaa gattatttaa cctgaccatc ataaatgcta
    38641 ttttaaaatt tcacttccgc aggcatccct atttttagcg atccgtcatt cggaacatca
    38701 attttaatgg catatacaag gttcaccctt tcatctctgg tttgaattat ctttggagta
    38761 aactctgcct ttgaagaaat ccatgatacc tggccatcat actgctgaaa gctttcatta
    38821 gccccatcaa taaacacttt tactttttgg ccaatcttca cctgcgacag ctgagatccg
    38881 gatataaaag ccctgagttt catgaccttc aagtctgcaa ttttgcaaat caccttccct
    38941 ggtgtgatta tctcgccctt ttcaacgtat ttgtccaata ttgtgccatt gacaggacta
    39001 acaaccttat actttgagag ttgatcggct atttgggcta tttgccattg caaagcctta
    39061 atttctgcat ctattgagtg catttgtgtt gcaacagcat ttttttgttt agctataagc
    39121 tttaactggt cattaatgtc gtcaagttgc ttaaccgggg cagatccgtc agacacaagc
    39181 ttctcaatac gtattttatt cacctcagcg gcccttaact gttcgtcata cacatccaac
    39241 tgagatgcta cttgcgtttt acgtgcacgg gccgactcaa tctgagcttc caactggttt
    39301 atacgcaaca ccgttgaaac agagtcaata gaaagaagta catccccttg tttaacctcc
    39361 tgcccttcat caaattccag ctcagtaatt ttaccctgaa cctcagaggt aatttgagtt
    39421 tcaacgactt caaaatttcc ataagcatcg gttttaccat ttcggttgtt gcacgacaca
    39481 aacgccaaaa cacttatcat cgctacaaat tttattattc tcattgtctt tattttatcg
    39541 taatttttaa ttcgtaattg aatattaagt ttaataaacc ccataaatgc tttgtaaatt
    39601 gctttgttcc tgcatgagct gaactttatg catctcgagg ttcaacttgg cttgcatttc
    39661 ggcattcaag tcattgataa aatctgtaga agaaatcagg cctttatcaa gcctgtctgc
    39721 cgaacgccgg gcaatggagc tgcgcagctc aacaatctta gcatctgtaa caacagcatt
    39781 ttcgagctgt acaatgcgtg atttgctatt attgatagat atttgtatgt tattttccac
    39841 tgcactgcgc tgattattga tcattacagc ctgcatacgg gcaatactcc tttcccgggc
    39901 tgaattgttc caatcgaaaa tattccactt gaaattcagg cctattatat agtaaggatc
    39961 aaaagaagta gaaagcatat tcaatcccgg tttaccatac cctgcctgag caaatgcaga
    40021 cagcttgggc atacgctggg ctttatatat tttttcagaa tattgcaggg cttcttgttt
    40081 tgcatccaat gcagccaaat caggacggct catttgcaaa ctatccggct tattaataac
    40141 aggtatatca aactttgccg aatcgcctat ttgctcgccc atcaataccg aaaggtttgc
    40201 caacaatgat ttataaagca tatctaattc aaatagctgt tgattaagtt tcaaaacctc
    40261 tacagtaatg ttatctctgt ccgactccat catcatcccc tgtgatataa gggaatttat
    40321 ctgcttcaat tttgtatcat aagtatttat aagcacttca accagctttt tattcgaacg
    40381 gttaagaata atggaataat acaactgagt agtttggtca agtactttat ttatatccga
    40441 tttagcgcta aatacatccg atttatagga taaatcagaa aactcacgcc gcgccttgtt
    40501 caaacctccg tcatatatca attgcgtggc atcaagccaa actttgtatt ggtcacggtc
    40561 tgtttcaatt gtttttttgg aaaacccaat tggtttctga aaagtatcag gtagcagcat
    40621 atcaagttta atagcatcac tctgatatgt agcttgtcca ttaaggttta actgaggcag
    40681 ccactcattt ttcaaatttt ttccctttaa acatgcaata gaatcggtga ttgtagcatt
    40741 agggccaatc ggatgatttt gccgcaccct ttgtttgcag tattcaaggc ttattgcttg
    40801 tccataaata aaagttggca caaaagcaaa caaaaggact acaggtattt taaataatct
    40861 cctcataatt actttttaat tgcattgatt acaaactcgg caacagcctt tttccgttct
    40921 tcaatgaaca aattccaatc gccagaatta ttggcaaata caaccccttg tattaaaggc
    40981 tttccaataa ccgggaaaat acacattgaa acaatgttta caataagatg acgaaaatca
    41041 ataggcctaa taacgccctt agctatattt tcctggatcg acattacaaa aggtgccatg
    41101 acattgtttc tcaacatatc aattttatca atcataagtt tcttaaacgc ttccgagtct
    41161 ctatttatct cctggacaac aaaaaccgga atatgtggat tgtcagaaaa aaaactaatg
    41221 taattatcaa cagccacctc tattctttca aagagagaca tatccttaga caaagtgaaa
    41281 gccatatttt caaaaaactt atcaattgtt tttgaaaaca ccatttcaaa cattttatct
    41341 tttgaacgat aataatagtg tagcaatgct ttgttgatac cagccttatc cgagatatcc
    41401 tgcatacggg caccgtaata acctttttca aaaaaaactt ccattgccgc agcaaatatc
    41461 tgctcttcag tttttaaccg ttgttcgtct gtcataggct ataatttttt agcaaatata
    41521 aattaactat ttggttaaac caaataattt atccaaaata ttaaattttt tagttaatac
    41581 tttcatacct cttttaataa tcgcttaatt acaactaaaa acgctcaatt taatatatat
    41641 atataacaaa aaatatataa ttaattacat tttttaatta ttaaacatat ttatattatt
    41701 ttttgtaaca taaatatgtt atttataata attttttgaa ttttatatta aaaataacat
    41761 cattttggat ataaaattat ttttataata aataaacttt taataacact ttatttatat
    41821 aaatatattt tcatttttta ctttattttt taaattatta tcacttttta tgtgttattt
    41881 taaaattaat tactattttt gtcttgaaat tattaaccgt aaaatatatt tattatgaaa
    41941 acaagtttaa aagtgatggt ggtagcagtt gctacaatgg tatcaggagc agtttcttta
    42001 gcacaaacaa ccgtaacccc gggattggat ttgtatagca cttatctttg gaggggctca
    42061 aaaattgatg ggccttcggc ccaaccttcg ttggttgtta aaaatggcgg attgacagtt
    42121 gggacatggg gctctttcgg attggcaagc tcatacagcg aaattgattt ttacgtttct
    42181 tacgcgtttg agtttggcct ttcattgggc gtaaccgatt attattatcc aaccttaaac
    42241 gctgacgggt atagtgcatt tgaattttca aaagattcag gggcacacgc tattgaagca
    42301 aacgttggat atacattgga caagtttacc ctatcggcca actacatctt caatgaagct
    42361 gccgcagctg cttcaaccgg tggcgacaaa tactttgaag ccagctatgc ttttgaaagc
    42421 tttagtttat ttgtaggtgc cggagacgga tggcatacca aagacaccaa atttgccctt
    42481 tgtaatattg gcgcaaaagc aacaaaagaa atcaaagtct ctgattcatt ctcaattcct
    42541 gtaacaggcg ttttaatgat aaacccggac aaggaattaa tcagcttagg cgtaggtatt
    42601 tcattttaac aaataccctt tataaatcac caatgaaact cccatatcct tgtaaaataa
    42661 taatataaag taagcactat gagagtttca ttattcatta aaatttaaat tttacaataa
    42721 tgaaaaagat tgaagctatt atccgtaagt cgaaatttga agatgtaacc aatgcccttc
    42781 atgaagctga aatcgacttt tttacttact cagaggttgt cggagttgga aatgaaaaat
    42841 ccggtgcaat atatagggct accgtttatg aaaccaggtt tatacctcga atattaattt
    42901 ctttcgtttg ccgggaccat ttggccgaaa atgcagtgca ggcaatagca acagctgcca
    42961 gaacgggcga acttggcgat ggcaaaatct tcatatcaac tgttgatgat tctgtccgca
    43021 taagaaatgg agaaagaggc cctgaatcat tgtttattaa agattagtgt aaatataaaa
    43081 aaagatatta tggattcaac agcattaaca ataggtttaa acaccatatg ggtgttaata
    43141 gcaggagctc tggtgttctt tatgcaaaca gggtttgcct tagtcgaagc aggttttacc
    43201 cgatcaaaaa acacgacaaa catattattt aaaaacttaa tggacttttg tattggcact
    43261 ttcgtctttt ggctattagg ctatggaata atgtttggtg ccggaaacgg gttcatcggg
    43321 ggatttgaat tgttctcaaa agttgaccac ggagctgcag caggattgcc caacatggca
    43381 ttctttttct ttcaattagt atttgccgct actgctgcta caattgtatc gggtgctatg
    43441 gcagaaagaa caaaattttc agcatactta atatatagcg ccataatctc gttaattatt
    43501 tatcccattt caggccactg gatatggggg ggaggctggt tgtcacaatt aagtatcccc
    43561 atgcacgatt ttgcaggatc aacagtagtg cattctgtag gcggatggtt agccctgaca
    43621 ggagccattg tattaggccc tcgcattggt aaatataaaa atggaaaagc aaatgctatc
    43681 cctggtcaca gcataacttt agcaacttta ggcgtattta tcctctggat tggttggttt
    43741 ggattcaacc caggttcaac tttaggagtg ggcgacgcag gcttgatttc aaaaatattc
    43801 gtaactacaa atgctgccgc tgcagttgga ggcattgcca ctatgatttt gacatggttt
    43861 agatatggaa agcctaccct ttcgatgaca ttaaacggtg ttttagcagg attagtagcc
    43921 attacagctg gttgcgacgc tgtaactccc ggaggagctg ttattatagg ttttatagct
    43981 ggaattgttg tagttttctc agtagaattg ttcgataaag tatttaaaat cgacgatccg
    44041 gttggtgcaa tatcagtcca cggaatatgc ggggctcttg gtacattatt agttggcatt
    44101 ttctcacaaa gcggaggcgt tctttacggt ggaggctggg gattatttac aagccaggct
    44161 attggagtag tatcggttgc tgcatggact attgcagtag gcttcattct cttccttgcc
    44221 atcaaataca ctattggtat ccgtgtatca gcaaaagaag aagaaattgg gctcgacttc
    44281 ttcgaacatg gagaaacagc ctacaacaac cattaaaaaa ttgtactaaa aatacacttg
    44341 ataaaaaatg caccttttgt ggtgcatttt tttatcaaga caatcaagaa cataaaatac
    44401 atgaagcaac atagttgcaa tatatttaaa gacccttcga aataaggtgc gtagcaccga
    44461 ataataattc gcctctactt accttaatca atgtaaagct gatttttctg caaatattta
    44521 tacacttcac agttaaaaga agattattac taacctgtcc aagtaaaact aaaaaagaaa
    44581 aagaatatat ttagtaaacc tatacccaaa aaataacctt agccatattt gaaaaacagg
    44641 ttacctaaaa atataaaagt tgaaccagtc tgccaaatat aaaaaccatg cttaattaaa
    44701 aatcaaattt ttatatataa tattattaaa atatatagga gtattaaaaa taattaatca
    44761 aaataacaaa tacaacccca atataattta tataaacaaa aacgatataa ataaattttt
    44821 atatttatca tcaaattgct tgataaatta aatcaaatta tataattttg taacaaatta
    44881 ttaataataa attaaattct ttaaatttat gaaaaaaatt gaagcaatta tccgtaagtc
    44941 aaaatttgaa gatgtaaaag ccgctcttca tgccgttgac atcgattttt ttacctactg
    45001 ggatgccact ggtgtaggaa aatcaacaga aaaaattatt tttcgtggca aatcgtatga
    45061 tacaagtaaa atcaaccgga ccatgttgtc aataatcgtt cgcgaccaaa atctggaaaa
    45121 aacaataaat gccattatta catctgccaa aaccggagaa atgggcgatg gaaaaatatt
    45181 cgtttcagac attgaagacg tttatcgtat cagaaatgca gagaaaggcc ccgaggccct
    45241 ttatataaaa ggtgaagaag tataattaca gcaacagaaa tgattaaccg taaaatattt
    45301 atatcatgaa aactttatca ataaaaacaa taggcttaat acttttggtt ttcttttgca
    45361 ctttaggagt tagcgcacaa gatcctaaag ctgcaaacga cagtatcaaa gccaccacag
    45421 aagcaactat tgctccagta tcaccttcag tggactctgg agatacagct tggctcatcg
    45481 tagcagcagc tttcgtcctg atgatgacca tacccggatt agccttgttt tatggcggat
    45541 tggtacgacg taaaaacgta ttgaacgtac tgatgcaatg ttttatcata accgctgtta
    45601 tcactatcga atgggtagtt attggttaca gtatgtcctt tggctcatct gaaggaattc
    45661 taaatccttt cataggaggc tttgactggg ctttcttaaa aggcatccat gtaagcgacc
    45721 tcagccccta ctttatctca cactcacaac caacggctga tggaggtaca ataggcacaa
    45781 tacctcacat tgtatttatc ctcttccaat gtatgtttgc tatcattacc cctgctctca
    45841 tattgggagc ttatgcagaa cgtattaaat ttaaaggatt tcttttgttc accgtgttat
    45901 ggtcgctgat cgtttacaac ccagttgcac actgggtatg gagcggcgac ggatggttat
    45961 ttaaaatggg ggcactcgac tttgccggag gcacagtagt acacatcaat gctggtattg
    46021 cagcccttgt aacagcgctg atgattggta aaagaaaata ttacgaagga catcctactc
    46081 aaccacacaa cattccttat gttgtaatgg gagcagcgct gctatgggtt ggctggattg
    46141 ggttcaacgc tggtagtggt ttggcagcag acggattggc agcaagtgca ttccttgtaa
    46201 cacacttggc cacagcaaca gctgcattta cctgggttat cctcgactgg attttcaaca
    46261 aaaaacctac cgttgttggt attgccaccg gtgcagtagc aggattggta gccattaccc
    46321 cggcagcagg ttttgtagat gtaacaggtg cattactaat aggtatgttg gtttcagtat
    46381 tctgtttcat catggttggg gtaataaaac ccaaacttgg atacgacgat tcgttagatg
    46441 catttggggt acacggtatt ggtggtttgt ggggtgcttt agcaaccggg ctctttgcaa
    46501 caacagccat acaaggctcg tacagtggat tgttttatgg aaacgcaaca cagtttatca
    46561 ttcagttaaa agctgttgga gttactattc tttactcagg aattcttacc gcagtgctat
    46621 tctggattac agataaactg gttggtgtta gggctaaaga tgttgacgaa actgaaggtc
    46681 ttgacattac acaacatggc gaaatagctt acaacaaagc agattaaacc ctttctcaaa
    46741 attagtttaa tacccgagtt gaaacggaaa ctatatattg gtttccgttt ctttttttta
    46801 ttatcactaa taaagttaac tattcagaca aacacaatga taaaataatt aaataaaatt
    46861 ttaaggttgg aataaaataa aaaagaattt cattacaaaa aataaacttt cgatatatat
    46921 tattaattat atttgcgcct caaattacat tttataactt ttaaataaaa ataaaatgaa
    46981 aaaacgcatt tggggtatta ttttattatt agtattttta aatctctttc aacaaaattt
    47041 gttttgccag tctgtttcgc aacaagaaca ggcctcatca attagccttg gcaatacagc
    47101 ctgggtgcta atctccacag ccctggtcat gttaatgact attccggggc ttgctttgtt
    47161 ttatggcgga cttgtccgcc gaaaaaacct cctgaacatc atcatgcaat gctttattat
    47221 tatgttgatt attagcattg aatgggtagt tttgggatac agcatgtcat ttggttcatc
    47281 cgaaggtttt ctaaaacctt ttatcggggg ctttgactgg gcatttctta acaacataaa
    47341 agcatcagac cttagtcctt atgcagttac tatgcctaac gcccgaatac cccacgttgt
    47401 atttatcatg taccagtgca tgtttgcagc cataactcca gcgcttatca taggggcttt
    47461 tgctgaaaga attaaattta aaggttttgt aatattttca ttgttatggg cattattcat
    47521 atacaacccg gtaacccact ggatgtggtc ttcagacgga tggttattta aaatgggggc
    47581 acttgacttt gccggaggca ttgtcgttca tattacagca ggtatctcgg ccttaattat
    47641 ggccatcatg cttggttgga gaaggtacta caacaacacc cctactccgc cccataatat
    47701 tccttttgtt gccataggaa caggaatact ttggtttggc tggttcgggt tcaatgccgg
    47761 aagcggattg gccgctgatg gagtagccgg aaatgcaatg cttgtaacac atatcgcagc
    47821 agcaacagca ggctttgttt gggccctgct cgattggttt attaacaaaa aacccacaac
    47881 cataggtgtt tgcactggta ttgttgccgg attagctgct attaccccaa cagcaggatt
    47941 tattgatgtc agggcagcta ttattgtaag tacaataagc tctattacct gtttcatcat
    48001 ggtaggttat ataaaaataa aacttggata tgatgattct ttagatgctt ttggcgttca
    48061 tggtattgct ggtatcattg gttctttact ggttggtatt tttgcttcac cagcaattca
    48121 gggctcttac cagggatttt ggtatggcaa caagcatcaa ttaaccgttc aactcattgc
    48181 agttggtgca accattgttt attctgccat tatcaccttt ttgctatata aattagtgga
    48241 caaattcttt ggcttacgcg ttaacccaaa tgaagagaac gaagggttag acttttcgca
    48301 acacaacgaa atagcctata acgaaacaga ttaacaaaca tccagttttt ataagtaaac
    48361 aaaagccgtt tcgtaaatct ctgtttatac ttccaaagtc attgaaatgc tgtttaatta
    48421 agcccaatta cttcatagct ttgctaaccc cagccttaag actggggtta caaactctaa
    48481 cttaccgacg gactttaacc ctgatatttt accggacaac agtgaataga agtaaatatt
    48541 tgaactgggc ataaaaaaag ccgcctcaag ctgaagcggc tttttatttt attatcttac
    48601 tcttatggca ttatctgaac ttttgcactg gccagttcat ttaaagaaac tgtaggtggt
    48661 atttgattat gcaataattt ttctttgacc agataaccta ttaccaatgc atctctttca
    48721 gaatgaaaag tttgttggtt ttgtatcgcg ggaatatttg tctgatatat aatagttttt
    48781 ttaccaaaaa ccaacttgta cccccagccc tcatcaacaa caaacgactc tacctttaag
    48841 cttttttttg tctgacaaga cacaaaagca aataaaaata aagacccaac cagtaaaaat
    48901 tgtttagtca tttgtattac tttcatcatc gggcataaat ttccagcaat catcaaatct
    48961 gtaactgctg cttgtgccag tagcaacaat tgccttatct gaaaatgtca aagctacggc
    49021 acgttgacgg gctgcccctt caaaatatga ttttttaacc cacaaatcgg tagtaggatt
    49081 gtattcccaa gtatcagaca aaatcgagcc attacttccc cctgttaaat aaccttttga
    49141 attaatagag aaagcggcgg catatgcacg aataatttta taattatcat cgtagctttc
    49201 atctttatta ctatccttta tatctctttt cttagtccaa acaccggaat caccctgagg
    49261 ggtaaacata taaaaatcat aattataagt gtcattatta tagcctccaa caacatatgc
    49321 atttccgcca ataataaatg atgaagcccc cattcgctta gtccccaata tactggtaat
    49381 ttgcctccac gaatcagttg ctgggtcata cctgtaaaaa tctttcaacc agcgatcgtt
    49441 ataaccagta ccaacaaatc cgtaattgtt taaggcaaaa cccacagcat cagagcggcc
    49501 aagagttgta tcaccattaa caatcaaatc tgcttttttt gtccatgaat tagaagaagg
    49561 atcgtaagcc cagaagtcat tcaaataatt gactccatca tagccagtac caatgtaccc
    49621 aattgaacct atcgagaatc caacccctgc actgcgtggt gttcctgtaa aatcagcttt
    49681 ttgagtccaa taatcaccat cattattata ttcccaaagg tctttaagcc tgttttttcc
    49741 gttatatccc atgcaaatgt aacccttgtt accaataaca aaactcattg cttcacttct
    49801 ggcaacgcca tcaaaatcag acatcctgac ccaattgcca ataagatcct ctgaatcatc
    49861 gtcatcatca gaacatgcag taaacaaaga aaccaatgct actataagca caccaaattt
    49921 taagtatttc atcattttat aaagatttta aaaacgtttc catcaagatt ttaatggcga
    49981 caaatgtatc ttaccatata cttcttaaaa aaaaatatct actaaataaa ttattttata
    50041 gttaatttgc acaaattctt cggcaagcgc aaaataaata aatttaaatt aaataattac
    50101 tttaacatga atatttattt attttgtcga gagttttaag aggttcatat agattattag
    50161 tttataaagg taaagcttag tttatttgcc ttacaagatt tttggaacaa tgaaaaaaca
    50221 acacgtagta tttttatttg ccttttttta ttttttagtt cattcatgta aatcagacga
    50281 tttttcgatt ggtagtgaag attttaactc aagcatcagg tcggtattta tagatacatg
    50341 tacagtcact ttttcgatgg taaaatctga ctcgtttgta acttcaggtt taacaactgg
    50401 ttggttaggg agttaccatg acaaatatac cggcaccctc actgctactc cttattttac
    50461 atttagccta ccttcatata cagcgtatga aaaaactttt ttcgattctg tcgttttgta
    50521 tgtaaaatgc ttaaacgact attttggtga cacggcactt attcaaagct ttagggtata
    50581 tgagcttgat gacaatattg aaaccgataa aaatgataat ttatacagca ctaattcttt
    50641 tgcccgaaaa acaaaagaat tagcaaatta taaatttaaa ccccaacctt acaaaaagaa
    50701 atatctttcg gtaaagcttt ctgatgattt aggaaaggat atgttggata agttcatgtc
    50761 cgaatctgac gaagtaagtg acgatactaa atttagcaat tatttcaagg ggcttgtgct
    50821 ggtccccgat gtatctaagt cgaataacgt tgtaaatatt gaagtttctg acacttcagt
    50881 ttcgatgata atatactatc acaggtacga agacaaaaaa aagaacgaaa caataaatat
    50941 ttcattaaat tccagtttgt tctttaccca cattgaccat gacaaaacag gtacaaatat
    51001 tgaaaagctc aactcgcaat atggtgagct gtcttcatta ttaacaaaca acgaagttta
    51061 tttacaaagc atgacttcta ctactgtccg tattgatttt ccttatataa aaaacctcaa
    51121 gcaattaggg gactatatac atatcaacag cgctatactt tatttaaaac ccctaaaaaa
    51181 ctcttacccg tttttatcgc ctttacctga aaacatttat cttagctaca ttgacgaatc
    51241 tgactacaaa agcgatcagg tagcctttaa actttcgaga gatttatatt ttaatgattt
    51301 aaataccaat tattccgtca gcattacaga tcttattaaa aatcaaatca atgcaatgag
    51361 tattgacaaa ttaaggatta accttaacct aaaaaaagat ggttcccaga gagatttaaa
    51421 gaggttggta ttaggagatt tttacaatga aaaagcaaaa gcttatgttg aaattcagct
    51481 ttctatatat aatgatgaaa attaaaagat attacctggc ttcagttttt attgccacta
    51541 tattattggt caatcaacct gttcaggcac aaaactcata taaccctaac tccgcttacg
    51601 gaattgggga aaccaatctt ggggctcaag gatataatgc aggaatggga ggcgtatcga
    51661 taggcgtgcg aagtaaagat tttatcaata cagataaccc tgcagccatt tcaattctgg
    51721 acagctcggt ggtactgttt aactttgcaa tgaacgggaa gtactccaag tttgcagcaa
    51781 cgggcaataa ccagaccgcc tggaatggca acatccgaaa tttaggcctt ggattgaaaa
    51841 tagcccgttt ttggggcaca gcactttgtt tattacccta ttcgtctgtc ggatatgaca
    51901 tttacacctt acaacctatt gaaggctcta aatatgtaga agctgcaaat tatattggcg
    51961 atggtgggat aaacagaatt caatggataa atgcatttaa aattcatcca aaattttctg
    52021 tcggggtaaa tgcttcatat ttctttgggg ctatcaatac aagacaaatg gtcaattcat
    52081 ggacgataaa aaaagtctct tctgtacaaa aattatactt tgactttggg ctgcaatttc
    52141 atgacaatat ctctaatgat ttgagttata ctgttgggtt agtttacgga aacaaaagtg
    52201 agctttcctt taaaaatact atttacacaa cagtaagcag caccgaaatt gaagaccgga
    52261 caacaaaatc tacaacacaa tatttgcctc agtattttgg cggggggatt tcttttacca
    52321 tgaatgaaaa aatcagcatt gcagccgatt accaatacac gcaatggtca tcgttaaata
    52381 aaactattgc aaatgttaaa taccgggatg ttagccagat aaaagcaggt ataaaagttg
    52441 gcccgctgca ggaaataacc acaaactacc taaacaggat acattacatg gcagggatag
    52501 ctattagcaa ctcgtatatc gaacgaagcg gcgttaaccc taaaaatttc ggtataacag
    52561 caggtctggg tttgcccata aggcaaaaag ccattgtaac aatttcggct gaacttggga
    52621 aaaccgggac tactgaaaaa ggcatggtaa gcgaaaccta tgttaggttt ggcgttggtg
    52681 tatcgtggaa agacatttgg tttgtcaggc gaaaatacga ttaaggtttt tctcatctgt
    52741 tgaaaacaga agtcttggca gcaattccaa accgaaggat atgtaaagta cggtaaaagt
    52801 agatgttata ataatcctgc ccgtaataat actgggcaaa aagatttacg tcacgaagga
    52861 aagatggctt atatgtcaaa gtatatttaa aagtaaaacg ctttttatcc caatagttta
    52921 cattcttcag gtcggttgca atatatccaa aacgaaatga attgttcagg acatctttat
    52981 tttcttcaaa ataagacaaa ttgaacaagt ttagcgtttt agaaaccaag ttccactccg
    53041 tttttaaatc taaatagacc ctcaagcgtc cgtaaatacc attcaattcc ttgtcttggt
    53101 tgtagctata ttctgagtac agcttcatat aatactttct attttccttg tttcgcgaaa
    53161 gaaaaagccc tgcttctaac caattggtcg aaaaacttcc atttcttgta tttattgcag
    53221 aactatctgc attgaagaat gggccatctt gcccgtttga gtggtgatac cacgaaaggt
    53281 aaacaaacca atcatgtttt ttggcattat ttacaaactg tcggaccaac aaaattcttg
    53341 gcatgtatga tggtgtgcgt acaggataag actctgcatt atacatcctg agcataacgc
    53401 ggggagaaag ccccatgccc catcgccagt ctttattaaa accaatcatg taataaggaa
    53461 tgatatctgc ctcaaacatc aaaggctcaa tattcccaac acctcctaaa cccgtgatat
    53521 aaccaggatc gcaaaacata ttaatggtat tgatagccga aatgctatcc aagcgttgag
    53581 cctttaacga aacaaaagaa aataacaaaa caaaaaatgt cagctttagc cttctcatcg
    53641 ctgataaaat taacaaacgc caaagttaac tgataaaaac aagattttaa ttaaaaaaac
    53701 tttctgagaa gataatttct gtaccaaaag tttaccaact gctggccatt ggcagagttc
    53761 cattcttgcc cgatatcgtt gtggcatgca atgccctccc cacgcctgct atgatgacaa
    53821 aaacggcatt ctaaaggctg aagccctgta ctataaagct cgcacaaatt gttttgcagg
    53881 aaagtacaac cattgtctga aaatatttgc aacgatacac cgccttcatt tcctttaaat
    53941 gcaggcgaca gtaccccaaa agtcctgtct ggagatattt ccagcatcat acgggctgca
    54001 tagcctctat ataaagcatt acaagcttgg tcaattgtcc accatccagg acgtttgcaa
    54061 taactcacac atgtcgggca tgaacatggt cctgacggcg agtatttttc aattagttgt
    54121 aaatgatcaa ctatactata cataaaaagt aataaaaagg ttcaaataag ttttggagaa
    54181 taattgaaaa ccaaaattat tatgccttac tttgaagata aatagtgatt attcatcatt
    54241 tttatgccgg tcaacttaaa acaacttacc gctacccacg aacaaaacag gaaaagtcac
    54301 caatgtattg tttcagaaga agattacaaa atggtcgagc ctcttttgaa gcttttacaa
    54361 cgtttgtcgg aagttgaaaa cagtaccatc tctgtttttg acatgaaccg aaataattat
    54421 ctgctaaaaa gcagtaaatt caaagaaatg ctgggttaca aaactcatga agatattgaa
    54481 aatgacgata cggccctttt tcatcaaata attcaccctg acgacctacc ttttgtgctt
    54541 gaaacggaga acaaagccta catttttttc aatagccttc caaatgaaga gaaaaaggat
    54601 tacaaacttg tgtatgactt tagggtaaag aatactgcag gggtgtatat gcgtttcatc
    54661 caccaattgg ctgttttaga acaagaccct tttggaaaat catggttgat aatgattata
    54721 acagagctga tagcccaacg ggctttaaac gttgtctcca aacgcaggat gataaacata
    54781 aaaaccaaga aactgcatat tttcaacgaa gacgataaat tgcacgcaga tatgctgctg
    54841 acaaaaagag aaaccgaaat cctaaacctt attgcacaag ggctggacag tcaacgaatt
    54901 tcggaaaagc tctttattag cgtaaacacc gtgaacaacc atcgacaaaa gatattgaac
    54961 aaaacgcaaa cggacaatac ctcgcaggca atcctttatg caaaaaaaat gggaatcata
    55021 taaaatgtac tacctatggc ttatgtagaa gaaaaaacat tcaacaaagt ggactttacc
    55081 agcaaagcgt tggaaaaagg agaatacgac aactgcacgt ttaaggaatg cgatttttca
    55141 aactcagacc tttcggatat caaatttctg gaatgcaggt ttgacaactg taacctaagt
    55201 atgacaaagc ttatcaacac ttctctctgt gatgtacgct ttaaaaactg taaaatgatt
    55261 ggtatgcatt ttgaaacgtg caatgaattt gggttttcta tacatttaga agattgtaac
    55321 cttagtcttt cctcatttta caaaatcaag atgaagaaaa acacatttaa aaacctgaaa
    55381 tttcacgaat gcgactttac cgagagcgac ttgagcctgt cggtctttga ccaatgcgat
    55441 tttgccaaag ccacatttga caataccaac cttgaaaaag cagatatgca aacttccttc
    55501 aattattcta ttgaccctga caaaaaccgc attaaaaagg ccaaattttc attaaacggc
    55561 attgccggat tgcttgacaa atatgacatt gagataaatt aagcaggcaa acccaatatt
    55621 tgaaatctca acatttgtac atttaataaa gaaatttcaa cattttgcca agccgaaaat
    55681 gttaaaatcc acgaacattg caataacgaa ttattaaaat ctaacctata cctaatcatc
    55741 taaaatttaa taaaatgaaa caaataattt tgtccatcag cttgctcatc gcagccaaat
    55801 gtatggcagc accagcagac acaatacctg ctaacagttc gcttattgaa tatactggta
    55861 gaattgactt ttccaaccca ttagccccca agttctcata ttctggagtt tctatcaggg
    55921 cttgttttac aggaacaagc atagcggcca ttatgaatga caatattggc aaaaactact
    55981 acaatgttat acttgacgga aaagtgacag atacaataaa tgtaagtatt ggacaaaaga
    56041 catatctgtt ggctgaaggc cttgaaaaca aagtacacga aattgaactg ttcaaacgta
    56101 ccgaacagga atttggaaaa accgagtttc ttggttttgt cgttgacaaa gacgcaaacc
    56161 tgtccactat tgtcaaccaa cgtgagaaaa tgattgaatt tattggcaat tcaatcacct
    56221 gtggctatgg aaacgaagga aaaaatggcg aaaaatttgg ccctactact gaaaaccact
    56281 acctgaccta cgctgccata acatcacgta actttaatgc ccgccatttg gccgtttgca
    56341 agtcagggat tggcatatac cgcaattatg atggcccggc aacaggcgat gcagagtgca
    56401 tgaccaacct ctacacacga acattccttt atgatgacaa gcctaagtat tcttttgcac
    56461 aaaaacccga tctcgtttgt attgacctgg gcacaaatga tttcagcact actggttgtg
    56521 actcggccag atttatcaac aactacttca ggctgattga caccatccaa accaaatatg
    56581 ccatgccaga gattatctgc ctggcaggcc ccatgatgtc cggctcagcc ttggtaacaa
    56641 taaaaaagta ccttaccttt attgccgatt ctgcaacaaa gaaaggcaaa ggcaaggttt
    56701 acttttttga aatgtctgcc caatcaggcg atgtaggtat tgattaccac ccaaatgtaa
    56761 ctcagcacaa aaaaaatgca caggagctta ccgattttat tgtaaaaact aaaggatgga
    56821 agattactcc acttgctgca agcgccagtt tgattggggc caaacatatt caggtagttt
    56881 tcaatacggg cattcaagat tcaactaaca attttaccgg aattgcagta gccggaaaca
    56941 atggtaatgt ttccattgac acagctttta ttgatactgc tgataataaa attttgcata
    57001 tacttacaga aagcagtttt gccattggcg aaagtgtaac attcagctat aatcagggct
    57061 ctataatgtc tatcgactct attgcattac tgacaattaa tcaatacaca atacaaaaca
    57121 aactcacaga aaccaagctg gcaagaggga tttctacaac tgatggtaaa tccatcttac
    57181 tttctttcaa taaagccctg aaaaacaaca ataacattga agggcttact attacaaaca
    57241 gtaaaggcat attagccatt gactcttttg ctatatcagg cacccaaata aacctttacc
    57301 ttaaagaagc tttaataaag ggcgattcaa tatttgctaa ttataatggc aatgccattg
    57361 taggttttga cgatatacca ctggaaagta taacaaaatt ggttgttaaa aatgcttcaa
    57421 aaatcgtaag tataacaaat atgaaaaata caactgtaga aatttttcct aaccccaata
    57481 aaactggaaa aatttattac cgcataaatc taacttcagg caacaaagga actattgaca
    57541 ttttcaacat taatggcaaa ttggtgttta gccaaagtat ttcaaaaaca gagggtttca
    57601 ttgacgctac caacctcaaa aagggaagtt acatcgtcaa aatcaaacaa ggagaaaact
    57661 caatatcaaa aaccatactc ttacaataaa aacaactcat tgaaaatgaa caactatact
    57721 tattataact aattaatttc tcaattatgg caaacacaaa aaagatgcat ccattagcat
    57781 ggttaatgct atttatgctt tgcatgaacg tgggggctca aaaccccaat ttttacatct
    57841 atctttgctt tgggcagtcc aatatggaag gccaggggac aatagaaacg caggacaaaa
    57901 ccgttgacag ccgttttaag gtttttcagg cgctgaattg ttcaaacctt ggaagaacaa
    57961 aggccaaatg gtacaccgcc gtaccgccaa cctgtcagtg ctactcaaaa ctctcgcctg
    58021 ccgactactt tggcaggaca atggtggcta acctccccga cagcattact gtaggcatca
    58081 tcaacgttgc tgtgggaggc tgcgatatca ggttgtttga taaagacata tacatggatt
    58141 atgattctac ttacaaagaa agctggttta cctctaaggt taaggattat ggctataatc
    58201 catataaata cctcatcgac ctggctaaac tggcccaaca agatggtgtt ataaaaggta
    58261 tccttcttca ccagggcgaa accaataccg gagatgctca atggccaact tatgttaaga
    58321 aaatatacaa cgatatgctt gccgaccttt cattgaaagc agactctgtt cccattctgg
    58381 ccggagaagt attggccgca gcaggcaatt gctgttcaag catgaatagc attattaaaa
    58441 ggctgcccga cactattcct actgcacatg ttatatcatc tgccggatgc gccggacaag
    58501 attatgccca ctttaattct gaaggttacc gcaaacttgg aagaagatac ggcgctacaa
    58561 tgctttcgct catggggtta gaagcagctt atgccgaagc agagtgcggc accgttggaa
    58621 aaaactggaa aactttggct gacaaaaatg cttcaaacgg agcttatgta acccaaacag
    58681 ccgcacaaaa caacactgcc tctgcgccct caagcgaaga caacatcatt caaatgagca
    58741 tttcggtaag cgctgatacc agctattacc tctatgggcg tttcaacaac gcaagcgcca
    58801 atagcaatgc attttgggta aaggtagatg atgccgaatt tgaattgttt gacaacctta
    58861 ccacaaccgg ttggcaatgg ctcgaattga aaaagctcga tttgactgcc ggagagcaca
    58921 aaatatatgt tggctttgcc aaagacagtg caatgctgga taaaatatgt ttgaaaaacg
    58981 cgcaaaaact gccggttgat gttgccgaag aagctgcaaa cctatgcacc ccctctatta
    59041 cacctgaccc aactcaggtt ggtgttactg ctaaagacgg gtacgcattg tatcaaaatt
    59101 ttcccaaccc ggttaacaat tgcagcacca tcatcacatt ccacattccc caaaccagct
    59161 ttgtatcttt aaaagtttac aattcgcaag gattggaaat taaagaactt gcaggaagaa
    59221 catttacctc aggcgaacat aacataacat ttaacatcga aaaattgtct cccggggcat
    59281 atttttatac tttgagagca ggacaatttt cttctaccca aaaaatgatt ataccagttg
    59341 aataatacat cacaaaattt aacccaaaaa ggctttcagc aatgaaggcc tttttgggta
    59401 tttatccttt aattgcttag aatatagctc ctgcttaagc aaacatctgt cagtccaata
    59461 tttgattaga aaacactgtt gtccggtaaa attaaataga tactggtaat atcgttgatt
    59521 agaaactggt cggccccgcc ggggcttaac tttaacgatg tttaattgta ctaccaacag
    59581 gacgtcgcta cgcgactaaa taatagcagc gtaactgcga actgtttgta ggacaaccaa
    59641 atacaccaaa tgtttctaag ctgcatagcg gcggcctgtt ggtaccggac aacaatgatt
    59701 ataaaatgaa cctgaacatt ttctgtcatt ctttcagaaa atatttcaat tctctttttt
    59761 ttctaaacat accttctcaa atgttcgcaa atttcggcta atgccaactt cgactctcta
    59821 aaaagattgc caaatgcagg atagcaatgg cacatacctt tatctacccg cagggttaca
    59881 ggtactccgg catctttggc cttttgggca aagcagatag catcatccaa aaggatttca
    59941 tttcctccgg cactgatatg caacgggggc aatcccttta aatcgccgta aagtggcgaa
    60001 atatatgggt tgcttctgtc atgccccttg gcataaaact cgctggcata ttgaggacaa
    60061 cctattggtg caaaacactt tttcatgttg gttttatacg acctgccggt aagcaacaaa
    60121 tctgtccaag gcgaaagcgc tgctgcagcc gcaggaagag caataccctt atctttcaat
    60181 gccagtaaag tagccaaaca taaccctccc ccggccgaat ctccggcaaa tataatattt
    60241 gaaggcttat aaccttcgtc cagcaaatat tgataggcag ccaaagcatc ttccaaagca
    60301 gaaggataag aatattcagg agctaaacga taatcaaaca ccaaagcatt gacacccgtg
    60361 ccttgtacaa acttaaccac gtgttgcctg tgcgattgag gagatcccaa caggtacatt
    60421 cctccatgaa aatacagcat cgccttttct gcatggttgc caggcaatct gacccattct
    60481 gcgtagcgtc cattaatgtt caactcgtct attacagttt ctttgggcaa tttgccaaaa
    60541 cgattggcag cctgctcaac cctttcgcgc atttttcgga tgccttccat ggatgggtcg
    60601 aaaggcttgc gcttcaattg aaaccgaaat aaatggctat ggagcatcag gttgagcaat
    60661 attttacttt ttaagctttg catattgttt acattaaaat tcatttaaaa acatattgag
    60721 aattatcctt ttgtcgggca ttttggcatt agcgccattt atagctttgt acatggccaa
    60781 cccgttgatg gtagaaaaga ataaggttgt catttcggct gcatcaaaac tcctgacagt
    60841 accttctttt tgcccatgaa tgaagatgcg gtttatgacc ccgtatttta tattgttttg
    60901 cgaaaagata aagtctctgg cttcctgagg aaaagcctca gacaaagcgg cttgagtaat
    60961 gagaaagtaa tagtaggccg tttccacttt ttggtcaaac ccttttaaaa gctgctcaac
    61021 agcaagcaca atcttctctt tcgccgacaa tggaagcttt tccaattcta atgctgctgt
    61081 attcatcttt tctactgcat cacgaattag ccagttaaac acttcttctt tggacttgta
    61141 ataatgataa attaagccct gcgaaatacc tgtttttgcc gatatatctg agatacgggt
    61201 tgcaaccaac cctttcctgg caaacaactc aagggctgca gcaagtatct gttcgcggcg
    61261 tccatctttc accttctggt ttgcttcttt attgcgtggc atatattgat tgattattta
    61321 ttcaatgcaa aaatagccat tttttttaaa gaaaaaacaa gtctttttaa ctaaaagtaa
    61381 atttcattcg ttaatacaat tgagtacctt taagcaatca acttcaaaat acgcataaaa
    61441 tgaacaacct aaattacgga gttataggca attgcaggag tgcagccttg gtatccagca
    61501 cggggagcat tgattggtgt tgtttgccag attttgattc accttcggtt tttgctaaaa
    61561 tattagacaa agaaaaaggc ggctatatgg catttgaagt ggatgattct tataaagttt
    61621 cacaaaaata ttttcaccgc accaacgttt tgtgtacaga attcaagtca gacgaaggta
    61681 catttgaggt tatagacttc atgcccaggt ataaaactgc cgaaaatcaa tatttttcgc
    61741 catctgaaat ataccgctat atcaggccca ttagtggggc tccgattttt aaggtaaaat
    61801 atgccccggc gttaaattat gccagagaaa aagtagccca taacattgaa gacaaccggg
    61861 tgaggtcgta ctctgtttcc aaccccactg attgtattta tttatacagc agcattgatt
    61921 ttgaagccat actaaacagt acgcctataa aacttgagaa acaacagttt cttcttgtct
    61981 cgtacaacca aaagcttata gacattgaca ttaacagggt atatcttgaa tatcagcgga
    62041 caaaagtata ttggctaaac tggacaaacc gttcaaaaaa atacgaagaa tatactgaag
    62101 aaatcatcag gagtttgctt gtgttgaaaa tgatgacata tcagccaaca ggcgcaatat
    62161 tggctgcgtt gacaacaagc atccccgaaa ctataggaga agtgcgaaac tgggattatc
    62221 gtttttgctg gctgcgagat gcctctatgg ccatagatac attgctaaaa atggggcact
    62281 acaattcggc caaagggttt atgacgttta tcaaaggcat tctcaaatca aagcatgaca
    62341 cgtttcaaat catgtatggc atccgtggtg aaagaaacct gaccgaatat aacctgatgc
    62401 acctttcagg ttacgaaaac tcaaagccgg tacgcatagg caatgcagca tactatcaaa
    62461 ggcaaaacga tgtatttggg taccttttaa atgttattta ccagtattac gaatattttc
    62521 ctggcacact ggacgagatt gaagatatgt gggaaatagt gcgcaatata gcccgtacag
    62581 tttctacaca ttgggaaaaa ccagacaaag gtatttggga aatacgcaac aacgaaaaac
    62641 actttgtatt ttcaaaggtg atgagctggg tagccatgga cagggctact aaaatagcta
    62701 ccatccttaa caagccgtat tattactgca attggagaga gattgcaaat gacattaaaa
    62761 atgatgtttt aaaaaatgga tggaaaaaag aaattcaatc attctcgcaa gcttatgaca
    62821 ataccgagtt ggacgcatct ttgctgctaa tggccgaata tggtttcatt tcgcatacag
    62881 acgaaaaatt tataaacacc gtacaggctg taaaaaaagc actttctcac caagggctta
    62941 tgtaccgcta taaaaatgaa gacgactttg gcaatccctc gtcatcattc accatttgca
    63001 cattttggct gattcaggcc ttatatcaaa ccgggcaagc ggctgaggcc aaaaacatct
    63061 tcgacaacct cctgcaatgg agcaaccacc ttggacttta tagtgaagac ttagattttg
    63121 aaacaaaaag gctgttgggc aacttcccac aagtatattc gcacctggct ttcatcaata
    63181 ctgcaacaat gttttcaaaa gaaaaaatta tttcacggtt tatcaagcca tgatttttaa
    63241 ttgtaaaaaa caataaaaaa cgttatacga caatctgaaa aatatgggag caaagagcgt
    63301 tatccgaaaa gtattacact ttttcaattt agagtcagaa ctggaagaac aggattcggt
    63361 ttatgaagag ataaaaaaag ggattatttt caaaggaacc aacctttgga ttttgatatt
    63421 tgccatcatc attgcatcta ttggcctgaa catgaattca acagcggtaa ttattggggc
    63481 aatgcttatt tcgccactaa tggggccaat caatggcatg ggatacagca ttgccaccta
    63541 cgattttgaa ttattcaaaa ggtctttaaa aaactttgct tttgctatta tctcaagctt
    63601 ggttgcatca acagcatact ttgcattaag cccaatatcg actgcacatt cagaattatt
    63661 ggccagaaca agccctacta tatatgatgt tttaattgct ttatttggtg gtttggcagg
    63721 tattgtagct attagcagca ggcaaaaagg caatgtcata cccggtgtag ctatagccac
    63781 tgcactgatg ccccccttat gcactgctgg ttatggtatt gccacaggac aattggattt
    63841 tttcctggga gctttttatt tattcaccat caataccata tttattgcca ttgcctcggt
    63901 atggatatcg caactgttga attttcctat aagaactatt atagacgaaa aaaagaaaaa
    63961 acacatcaac aggataataa ccctgattat ctctattgta ctattaccca gtattttctt
    64021 tgggtataag ctggttaagc aggaagaatt tatccaaaaa acaaaccaat acatttcggg
    64081 catttcaaat tttgaaggaa attacctgct gaaccataaa atagatgaaa aaagccatga
    64141 aataactttg gtttatggag gcacttccct gtctgatgag caaaaagatg caataataat
    64201 gaaagccaaa gattttggac ttgaagaaac tattatacac attgagcaag gaatctcatt
    64261 gggcaataaa gataacgaaa aaggagaatt attcaggtta aaagaagaaa taataacatt
    64321 aaacagtact ttaaagaaaa aaaaggcaac gcttgacagc attcaaaatt cacaccagac
    64381 aggacaagtt ttgctcaacg aaattcaaac tatatacccg caaataaaaa attgctctta
    64441 ttcaaaaacc tatacctata gtgaaaagca tgcatctcca acaagtacaa ccattatagt
    64501 tttaacaaca aaaggctcaa tcactgtttc tgaaaaagca aaaatcaata attggttaaa
    64561 aaaaaggctg aataatgaag ctgtgaaagt tttttatgaa taagcaaaag caaataagct
    64621 atggaagcaa attttaaaag gcttgttatt gcagcatatc ggttgccctt taagtttagc
    64681 aaaactaaaa atggctataa aaccatgcaa aactcgggag gtttggtttc agccatactg
    64741 gccttatctg aaaaatttaa aaagagcaaa gactttcggg taaacgaaaa aatactgtgg
    64801 gcaggtattg acaataatat ccccgatgat ttcccgtctg ataaacttaa caacaagcat
    64861 ttcaatatct gtccggtaca tatacctgcc aatactaatg agttgtatta cggaggcttt
    64921 tgcaatgacc ttatatggcc gctgtttcat tattttccta cctacagtgt atttaacaac
    64981 aactacttta aagcatacga agaagcaaat accttatttt gcaacgaaat aattaaggta
    65041 ataaaaccgg gcgactttat ttggatacat gattatcaac tgcttttgtt gcctgaaatg
    65101 ctcagaaaaa agatgcccga tgtaactatt ggtttctttt tgcacatccc cttcccttct
    65161 tttgaaatat tcaggctttt gccacgcaaa tggcgcgaaa gtattatcaa cggtatgctt
    65221 ggcgccgatt tggttggctt tcacacacat gactataccc agcatttcat caaatgcgtt
    65281 aaacgtacca ctggttttga atgccatcaa aacgtgatac ttacgcccaa taaactcgta
    65341 aaagctgatg ccttccccat tggtattgat tttgacaagt ttcacggcgc atgttttgac
    65401 aaacctgttg taaaagaaaa agagcaaata aaaaaacagc tttcaggcca aaaacttgta
    65461 ttttcggtgg acaggctcga ctataccaaa gggcttttgc tccgactcaa agggtttgaa
    65521 acctttcttg aaaaatatcc agcatggaat ggcaaagtca ttttcaatat ggtagttgta
    65581 ccctcacgcg acaacatcaa acagtacgag aaaatgaaaa aagagataga atcaaccgta
    65641 ggccgtatta acggcaaata cagttcatta tcatggcggc ctgttattta ccagtataaa
    65701 tctttgtctt tcaacgagct gatagccctt tacgatttga gtgatgttgg ccttatcacc
    65761 cctttgcgcg acgggatgaa cctggtggcc aaagaatata ttgcctgtca aaaagaaaat
    65821 aaaggcgtat tgatacttag cgaaatggct ggtgctgctg ccgaattgac tgaggccatc
    65881 attataaacc ctgttgacaa tgatgaaatg gcagaagcta tttgctcagc cttggaaatg
    65941 aaaactaacg aaaagcatgt acgcctaaaa cgcatgcagg acaggattgc caattacaac
    66001 gtcttcacct gggcgttgga tttctttgca caaacggcag atgccaaaat acaacaaaac
    66061 cagatgagtg ttaaatacgt caacaaacaa atcgtttcgc aaataaaaac aagctacctc
    66121 aatgctgcca aacgcatact atttcttgat tatgatggaa ctctggtgtc gtttgccaaa
    66181 taccctgagc tggccatcat tgataaaaaa accctatcta tcattaacca attgtcaaaa
    66241 gaccctaaaa acactgtagt tatcattagt ggcagggaca aaaactttct cgaaaaacag
    66301 tttgaagggc ttgatgtgac cctggttgcc gagcatggat attttgaaaa aaaacacggg
    66361 aaggattgga aagaaaccgt aaataccgct tcaaactgga aaagcattgt catgccaata
    66421 cttaaagaat atgtcaaccg ttgtaacggg tcatttattg aagaaaaaac gggcagctta
    66481 gcctggcatt accgaaatgc cgagatggac tttgcacaac tcaggttgca cgaactgcgt
    66541 gatgaccttg ccgagataat acggcacaaa accgaccttg agatattgga aggcaacaaa
    66601 gtactcgaaa tcaaaagcgg gaaatacgat aaaggacaaa cagccgccaa cctgctcaaa
    66661 aacaaaaact acgaatttat cctggccgca ggcgatgata aaacagacga aaccatgttt
    66721 tcggcattgc cagccgaaac attttctatc cgcataggca aatgttcgtc tttggcaaaa
    66781 tataacgtgg gtagctatat tgaatttata gctttacttg ccaacctttc tgatatgtaa
    66841 caatatcatt ttttataaag ctaatacatt actaaaaaag aaagtaccat acctccaatc
    66901 acaatccata ttacatccac ctttcgcaat agcaggacaa aagcaaccaa tactattacg
    66961 atattgaaaa tattccattg tacaccgata gtaaaacgat atgttacaac aactaacaat
    67021 cctacaaacg aacaaagtac gccattaata actttattaa actgaggata tgcctttagc
    67081 ttgtcaaaga aaggaataag tcccatcaaa ataaggaatg atgggataaa cacacacaca
    67141 gtagccagta tgctgcccag aataccaaaa tgcatatagc caaaaaatgt agctgcgatg
    67201 atgattgagc caggtgtaac ctgacccaga ataaccccgt ccatgaaagt tttctggtcg
    67261 aaccaaccat ataagtcaac catttcatgg tacataatag gcattgaagc tagtccacct
    67321 ccaaaggaaa acaggtcaat acgcaacata atagttgcaa gagtaaagta atccctgttt
    67381 acaagaaata taatacctgt agctacagca acaacagcca aagtccatat aaatagtttg
    67441 aaagactttg acttgactcg tttttttgat tctccaaaat cttttttagt aaaaactatc
    67501 cccaataaag ctgcaataag caataccaaa gccggatgca gcttagtaag aaacaaccct
    67561 gcagcaatgg aagcaataat ccagtcgttg tagttttcaa aatttttaga accaaacata
    67621 taagcagcat tggccactat ggcaacaata atcacccgaa ggccattcag cacaagctca
    67681 acattggaca ggtgcttaga atgcttgtat ataaccgaaa ggataaacat gataagaaaa
    67741 gcaggcaggc caaacccgat aaaactaaca atagccccgc gcgagccttt cagcttgagc
    67801 ccgatgtaag ctaccaactg cataataata gctccgggaa tcacctggca aagcgccaga
    67861 ccagaatcaa atgtattagc atcaagccat tgcttctttt cgacaatgtg tttacgcaca
    67921 tgcgccgaca tggctacacc tccaaatgct gtaaggccaa gccaaaagaa tgccctgaaa
    67981 agctcaatat aagatggttg atgttctttc aatatattat aacaagctat acatgatgat
    68041 tgttaaagat aagacagaaa taaaaaacca aagcaaaatg ccttgaataa agggctttat
    68101 gccaacattt tttaacaaat cacgggttaa acctgtacca atgagaaaaa gggtaatctt
    68161 tagcccaatt ttagaaatat tgacaataaa agcagcttca tgatgaaccg caggaatgaa
    68221 ggtattgatg agcatagcca aaacaaacag aatgataaaa tagggtattt tcaccttttt
    68281 gttatcgcct ttgacaataa atacggttaa aaaggcaata gggataatcc acaaagcccg
    68341 ttcgagcttg atggtagtgg ccacctgcaa agcttcttgc ccgtaattac tggctgcgcc
    68401 aacaacagag ctggtgtcgt ggatagcaat agcagcccaa acgccaaact ggtgttgcga
    68461 aaggttcagc caatgcccaa taattggaaa aatgaacaaa gccaatgagt ttagaataaa
    68521 aactgtgcct aaagcagtag aaacttgctt ttcgttcgac ttaaccaaag ccgaaacagc
    68581 agcaatagcg cttcctccac aaactgcagt tccggttgat atcaggtacg aggttttgcg
    68641 ctctactttc atctttttgc ccaaaaatat ccccaggcca atagttgtag caatagaaaa
    68701 aatggtaaat acaaggcctt gttttccagc ctcagcagcc tgaaaggtat tcatcccgaa
    68761 ccccatcccg acaactgaaa cttgtagcaa aatatgggtc agctttttac ttgtcaacaa
    68821 aaaagggtgt tttacagttt gtgccagtat aatacccata aataaagcta ttggagcatc
    68881 aacaaatggg aataagcata aaacaacagc cgtaacataa ataatccaag aattcctcat
    68941 tgtgttaaat tttactttgc aaacgtaaca caatgataat caacagtcaa atcacaaaaa
    69001 gtaataaatt ataactttaa gttataataa ttttgcaaga aattgacaaa cagcttagta
    69061 agccctgagg gttcaggtcc ctgaggtgaa ataaaataga attgacgttg aaacctaata
    69121 ttactggtat ttatttgcct aaaaatacca gaaagcagtt catgctcaat agcaaagcgg
    69181 gaaacaatac ccacaccaga gcctgtcttt acaaaagact ttatagcctc agtactacct
    69241 aaatacatta atacttgcag gcttttgagg tttacaccgt attgttgaag gctattttca
    69301 atgatttcca gtgtccccga acctctttcg cgcaaaacca aaggcaaaga agccaactcc
    69361 ttagcagaga caacctgtgg agcgctttta ttttgtgaag aagtaaatac aatgagttcg
    69421 tcatcaagga atggataata gcggatgtct gtattgccag gatttccttc aactatacca
    69481 aagtcaatat catttttcag caaccgtttt tctatttgca gcgtatttcc attgacaagg
    69541 gacagattta tatcggggta gcgctcgtga aactttgaca atgccgatgg tataacatat
    69601 tgcgagatgg ttgtactagc ccctatacgc aactcccctt tgctcttgcc ttttagccgg
    69661 ctcaattcaa acttaacttc gttatgcagc gattgtattt tttcgctgta cgaaagaagt
    69721 aggattccgg ccttagtaag ctcaatttta ttgccatggc gattaaaaag ttttgcttca
    69781 aattccgatt ccaattcctt aatatgtttg gtaaccgcag gctgagtaat atacaactca
    69841 ttggcagctt tagaaaaact taagttgtgg gccacacaga taaacacatt cagccggaag
    69901 tcaagcataa ctttaaatta taataccata aaagtaatat tttctatggt ttaacataag
    69961 ataaagacct ataaaaaact tacttcacca cttcaaggtt attggccgtc caaccactaa
    70021 agccgtccat catctggtac accgttttga acccgttctg gaccatgtga tctgtggcta
    70081 ttttacttcg cctgcttgta cggcaatata caatgtacct agctttgcgg tcaagcttat
    70141 taatcttttc aagagcacct tgctgtgtaa catcaatatt tactgcacct ttaacgtgac
    70201 ctgcctgaaa ttcaccagca gtgcgaacat ccaacacaac cagcttctta tcttttttaa
    70261 gcatagtgct tacttccatt gaattggact ctattgggct ttgtgctgtc aatgttgcaa
    70321 aagtgctgat aaacagagta actattgcga ttaatgcttt tattttcatg atgttaaatt
    70381 tttgagtaaa gtaaaaacaa gaagatgaaa gtattttgaa atactcccat cttcaattcc
    70441 tttgctgaca gctttcttat tctttctttt tacaggtatc aataccaaac gggatataca
    70501 acggacaaaa actgataagg cttgtcaaaa caaatactgc agcaagtatc agcaacacaa
    70561 tgcccaatgc tcctgaaata atatttgtaa agaataaaat agcaattatg actgcaacca
    70621 gtatcctgat taccttgtca atagtaccca tgttcttttt cattgtcaat aaaattaatg
    70681 gttaattatt aatggtaaaa tcgtaatttt taatttactg catcccttgc attccaaagc
    70741 ttttaaaata cgagaaccat ttttcttccg gattttgtat ttgtttcaag gttttcagcg
    70801 actcagcctc gtctatccct ttttccagca catgatacat gtgtataaga ttggaagact
    70861 taatgtttcc tccacaatgt acaaacacct ttttgccatc cagcccagcc atgatgccgc
    70921 taaaagtatt ataatgaaac gggcgcaaat tggagcaatc taccggaaaa tgtacgtaat
    70981 ccatttgcag cttttcaacc acctgggctt catctttcaa agcatttctg gtacttacag
    71041 gtgaaatatt tacaattgcc tcaaaacctg cagcttttaa agcctcaatc tgcgctgttg
    71101 aaggctgagc gccagatgaa agcaattcgt tatatttgaa atagtttgca atatccatat
    71161 cgttttattt gaaaatttcc attgggttat gcttgaactc cctgtcttta acaattaaag
    71221 tagtagatgg cgcttttgat tttgcagtaa aaatcatatc atggcccaaa cataagccta
    71281 aaacaacatt tagctcggtt ttgttttcgg ccagaaaatc tgcctggcca gcaggattgc
    71341 atgaaacgcc tttagcttct tcgcccaaaa actcgctagc agggattttg ccacatttac
    71401 aatccacagc aaatacttca aaatcttttg ataaggactc ttttagcata ttagcttctt
    71461 tctgcactgc cacacagttg gcaatgccaa tacgtttgaa accagcattt ttggcaaaat
    71521 tgcgaatttc ctctaccctg tttttgccgg gcaaacgcga ttggtctgcc agttgcatgg
    71581 tttgaatatc agaggctgaa taaagtgtta tttcgttcat tttgttatta atttttagaa
    71641 gttacagcct tcatagggca tatcatggcg cattttccgc actcaataca tttttcttca
    71701 tcaattacag gcagcccaaa gccatcctga gtaatagccc caacaggaca aacccttaac
    71761 ataggacaac gatggttttg cggacaggcc tgaggattaa caactaaagc catatacaat
    71821 catttaatat ttaacaaaat attcatcaac tggttttttg agtgtacccc tgcttgttta
    71881 tacttcaatt cgccatcctt aaaaatagca agcgttggaa ctccctgcac atgataccgg
    71941 cctgctatat cagggttctg atcaacatca atttttatca cacgcacttt atcgccaagt
    72001 tcagtcgcaa tttctttaag aattggcgac tgagctttac acggggcaca ccacaatgca
    72061 tgaaaatcaa caataacagg ccgggtatcg tttattaatg aattaaaatt ggctttcatt
    72121 ttttcatttt tattttaatg aaacacccat gattggtcaa acgcaaacac gatagtattt
    72181 acttgtgggt gttctcctgc taaagacggg acaagctatt attccttata aattaaattt
    72241 tattatgatg aaatgataac atttacccac aaataaattt cattatgttt ttaacaggct
    72301 caaagttgca attgagcaat catgagtact tcttcttaac aaatcatacc tgtttcattt
    72361 gttttatcag aaaatcttta gatttgaccc caacaaaacg gttcacttct acgccattct
    72421 ttagcagtat gagcgtagga atgttgcgta ctttaaattt ctgagcaagc gattggtatt
    72481 gctcgatgtt taccttgcct acatgctggt tgccgcttaa ctcgcctgct acatcattga
    72541 gtattggtgc catcatacga caaggcgcgc accaactggc ccaaaagtca accagcacta
    72601 ctctgccttt tgtttgttgt tgaaaattgg cttcggtaag cgtcaatatt ttgtcatggt
    72661 cttttaccat agggatgttt ttcagcctgc gctttgctgc gaaaaccaga taagcgaaaa
    72721 cgccaatcat tacaccaagc acaattaatg ttgtttgcat tttttaatta ttttataaaa
    72781 taaactttac tcttttcctt ccattacgaa gagcttgtcc cgcctcaggc gggaggttgg
    72841 ggaggtttat taaactcaat ccaattcctt agcaatagag atagctgcaa tagtcccatc
    72901 agccacagca gtagtaatct ggcgatattt tttacttatc acgtcaccta ccgcgtaaac
    72961 gcccggaata ctggttcgca tatcttcgtc tgtcttgata tatccccact ggtcaagttc
    73021 aagtttttct ttaaacaagt caatattggg tttgaagcct ataaaaatga aaactccatc
    73081 actgacaagc tttatacgct cttttgtttt caggttttca atctcagata taacctggtt
    73141 tccctcttta acaaacgagc ggggctcatg ttcataaagc actttgattt ttgggtttgc
    73201 caaaagcttt tcttgcgcct ttttgttggc agtgagctta tcaaactggt gaaccatagt
    73261 tattttagaa gcaaacttgg ctataaagtc cgattcttca actgcagagt ttccaccacc
    73321 tatcacaact acctctttat ctacaaaata tttagcatca caagtggcac agtaagaaat
    73381 tcctttgcct ttcagttcca tctcacccgg ggcgccagtc aggttgggtg atgtcccggt
    73441 ggcaatgata atgcgtttgg ctttcagcgt ttcgtattca tcaatgacga cctctttttt
    73501 ctcaaggtca accctggtaa catctacagc aaccttatat acggagccac aaagcttggt
    73561 ttgctcactc atgttgtgcg acagcatata gcctgcctga ggttcaataa aaccaggata
    73621 attagacacc tcgtgcgttg tagatacgtg cccgcccggc aatgcaatgt ccaccattac
    73681 agtgttaatc ttggcctggc ataaatacaa accggcagtc aacccggcag ggccggcccc
    73741 aaggataatt acatcaaact cggtaacggc aggttttatc tgcgatttaa tagccttaac
    73801 gcgttcgaca gggatcatgc tgtccaagtg gcagataata tcgctgcgct taataccacc
    73861 agacaacctg tcggccactt ctttcccatt atcaaaaaac aatagcgttg gcgaagattt
    73921 aactccgagt ttatcagcta attcgcggtt ttgttgacga aatattttaa aaaacttaat
    73981 atcattgccg taaagccggg ataaattttc aaattttggg gcaagcgctt cacatggcgg
    74041 gcattcagta gaataaaagt caagtactac tttgcctccc tgaaggactt cagtttcaaa
    74101 ttcagatgca tttatttctt tcatagcatt aattattaat taaaacaata cattttaaag
    74161 aactttactg attatcataa agttactaaa acaatactgt tttatctaag gaatcaaatt
    74221 aatttttcag caaaatttcg ttgatggcct gatcaaatgt ttgacgggga agagcgccca
    74281 tagcagcctg aggttgtcct tctttaggca caaagagaag ggttggaata cttgagatgc
    74341 caaaaactga agctaattcc tgttcgctct cagtatcaac cttgtaaaca ataagctttc
    74401 cggcataatc cttagcaata tcctgaagaa ttggggctac catcttacat gggccacacc
    74461 agtcggcata aaaatcaata attgcaggca attcgccaac gtatttccac tctttgttta
    74521 cctcgtaatt aaatactttt tctttaaatt cctgggtggt taagtaaacc acatctttta
    74581 ccattgtttc cataattaat taattttaaa ttatgaaaca aagatacgaa aaatgattga
    74641 ataaatcgct ataatatcac caaaataaat cgcaataata acacttttta agaaatggct
    74701 aaaaatatga tgcaaagccc agttcgataa gctccttcac cggacggcag tcggtagagc
    74761 agatttgccc tgattgattt ttagccaccg aaccacaacc acccccgcag gccaattgca
    74821 ggctgcagtc tttacaatca ttgatggttg ttacgtcacg tttttcccat tcattaataa
    74881 ggcttgtatt taaagccgtt tggggataga atgtccctaa cgactcatct gccttaccga
    74941 cagtggcagt acacgggtat atgtggccgg tataatcaaa agcccattcg gtcttacatg
    75001 cagggcatga gtcgaacaat ggggcaggca agctgccatt ttcggccaga aactttgaga
    75061 tagaataagc aggtttgtaa aactctacaa tataaggatg ctgtttaatt agtgaataaa
    75121 gcgattcgta caacgaaacc cttgtaaaca gcttgtcagg cgcagattgg caatggtgca
    75181 actcgtagtt gcggccaatc tgagttttga aatacgaatt ttttgtccaa cctttatcaa
    75241 tggcaaagcg ggcaagctca ggcaggttat tgatattctc cttgtcaatg accatgcgca
    75301 agttcacatt cagcttattg gcaagagtaa catctatgcc atttacaatt ttatcaaaag
    75361 tgccagcacc acctttgagg aagcgcctta cattatgcac agcagccgta ccatccagcg
    75421 taatctgaat ttcgcggatt cggccctttt tgagaatatc aatatattcg gccagagtat
    75481 atccattggt aacaaagcaa agctcaagcc caactttatt ggactgttca actaaatatg
    75541 taattatttc tttttgtttt ggagagttta acaaaggttc tcctccaaat accgttatat
    75601 atttgcgcct tccggcaaat tctttttgaa tatacgtaaa aaaggcatca acaatttcgt
    75661 tagtcagttc gctcttagcc ggggcgtatt cgtcctgata acaatacgaa caggcaaagt
    75721 tgcagctgta gttggggaca aagaacaact gcacttcatc gttatctcga ttgtcaataa
    75781 aatcaagata tttgctttta tacagcttat tttcttctgt ctcgtccacc caatagcctt
    75841 tggcaaccat atcgtttaca aaatcatcag aaacaatttc tttactattc ctgagcccgt
    75901 ctattttgca ggcttcgtcg ggcgacaaaa tatcggcatt gcccgaaagg aggttcacaa
    75961 taaagaaatt ctcagaatcc ttgatgttag aaaatatgtt gtgtttagaa aaaaccatct
    76021 aattacgaat tacagattac taatgacaga tgactaatta aaacaatccg attaaacttt
    76081 aaagaccttt tacagccttc tattatatag aaaatggcag ctaatttgaa ctaaccccta
    76141 tctccttgaa gaactgcaga taggactttg attgttaatt caaaatattt tttgccatat
    76201 tctataaaat tactttgtaa aaggagtaac atgcaccttc aaatttagtg ctctatttga
    76261 gggtgtgtaa aaagtctgaa aaggcttcga cttcgctcag cctgacgatt gataatcagc
    76321 aatttgtcat tttgagcgga gtcgaaaaaa tgaacaaatt tgaccttttt acacaccctc
    76381 gttggggtgg taaaaaaaag gcaccaggcc ttacttttag cagtgtcctg atgcccaaat
    76441 tcaattagtc gtggcagccg gcaacatttt tagatgtttt tgcgcagcac tgggctacct
    76501 tcgcgtttgt taacacttct ttttttctaa taagacttgt cataataaac agtttaaaaa
    76561 aattatttaa ctaaaagaac ttcattgata gcttttacta acgattcttt aggcaaagcc
    76621 cccatagcag cctgtggctg ccctttggcc ggaatgaaca aaattgtagg taagctttgt
    76681 attcccagat tttgagcaag ttgtctttca acatccacat ctaccttata aacaacaatc
    76741 tttcctgcat attccctggc tatttcctca atacgtggcg aaatctgacg acaagggccg
    76801 caccatgtag cataaaagtc aatgatagcc ggcatagtgc cttcatattt ccattgctta
    76861 tttacttcgt agttaaaaac cttttgttta aaggtttcgt ttgtcaagac aattacttct
    76921 ccaaatttgg ctttgccttc aggacttcct gcgctacaat tgccaagtaa aaaagcaaat
    76981 gcaagtaccg ataatgttat aatctttctc atgttattaa atttaaaaat atttttactt
    77041 tttacaacca catttagctg gtttttgggc tgcaatggtc cagctaacca ctttaatttt
    77101 acctttgtca taaggggcgg attcttcaat aatatttact gatgaaaagc ctgcattctc
    77161 aagctgacta agatattcgt cacgagtaac agagcctgcc cagcattctg agacagcaac
    77221 agggtcattt ctatattcat cgggtacggg gcttgtagaa tatatatcgc ttaccacaaa
    77281 acggccgcct tttttcaata tccggtatat ttcgttccat accatctgct tatccgaagc
    77341 atggttgatg gtgcaattag atataacaag gtctgcaaca gcatcgtcaa gcgcaatctt
    77401 ttccaattcg ctgcggacaa acttaacgtt ggtaacgttc aatctttttg cagtatctat
    77461 ggctttttca atcataccat cagaaatatc aataccgtaa acaaagccgt tttcgccaac
    77521 gctttcggca aggcgtatta cgtcagtacc tcgtccgctt cccaagtcaa cacatacttc
    77581 tccggctttt acctctgagt agttgatagc acctccgcat gacaagcaac aatcggactt
    77641 tgccagctcg ctgtatcttt tatttatttc tattaatgac ataaaagttg atagttattg
    77701 gttgttagag cttattcgta atttttaatt tttaattttt aaatcctcaa catttacctc
    77761 tgctacatgg gctgctcacg ataaaaaaac ccataaaccc tccaaaaatg atactcatat
    77821 atggactgct tgttatcggg caactaccag aagaacaacc tatataataa tagtacagaa
    77881 agcctgcaat cataccaata acagttgcaa taaaaggttt ccagaaatac caggaagaaa
    77941 aaaactcttt tatggtcttt ggcctgggct ttacttcaca attattttcc atctttacct
    78001 gtttttaaaa tttactctgc aaagataaaa caaataaaaa aataaatcac aataatatca
    78061 cctaaatctt tttaatatca ccaaaaaaat ttttaccttt gtaccctcaa tgtattgatt
    78121 atgagcaaag tttttgatga tagccttagt cctgaacagc ttgaagagat gttccacaat
    78181 gatgaaaaat gtcttgaatt tttggctaat ctcaagtggg ccaatggttt cgtatgcaaa
    78241 aaatgcggca acaccaactc ctgcccgggc aaagagccct tctcgagacg ttgcaccaaa
    78301 tgcaaaacaa aagagactgc caccaacggc acaatatttc atggtgtcaa atttcctatc
    78361 agcaaagctt tttacattgc atacaatgta tgtaaaggga aagaagacat ctcgtcctac
    78421 gaatttggcc gcaggctttc gttgcgccag atgacctgtt ggaacttcaa atcaaagata
    78481 gaacacgcat tagttgaaat gggctcttta acagacaaag aaagaagctc tattgaaaaa
    78541 atactgacca gctaataatc ttgtttttac aagagttcac ctattatagc ttataattca
    78601 tatataagag aaattccaaa aaatggatat ccaggatagt ttcttagctg gccataagga
    78661 ttaagtcctc ggtagccatg aaagtaaaat ccaactgcgt gattgaaatt tgtttttgga
    78721 taaaatttat atccccaata aagattaaag ttaaatagca ttccttcttt tacttcagta
    78781 gtttcccatt tcccttcatc tactttttta aacaatgggt aaccatatct caatctatag
    78841 cgaacttcaa atgataaaac attgacaaaa cgtttactgg caagaaagcc ttgggttctt
    78901 tgaaattggt attctaaata atattcaccc cgatatttac ttgctggaat atccaaatcg
    78961 gtagttaatt cggcatcttt acgtacactg aaccacccaa gacctctatt gctaattcgg
    79021 tatgaaaaac ctaaccttaa agagtgacag ttctccctaa tcctgtccgg gtcattaatg
    79081 gttgcttgaa attctgtgta ttcgtaagat acattaatac gcgtaattgg aaaattttcg
    79141 tctttacgat atattgttat ttcatcacct agatgtgtgc actcgtgaaa aattggaagc
    79201 caggaaaaag atatattttt gataaaccta ttgtttggga aatatcgtat taaccttaat
    79261 ttgaatgatc caaaacgata atcagtatta attacaggag ccgtttctga gccccacata
    79321 tcttcaagca catgaacgct aacgggtact gaagcggcaa aaccccactt gtttttattg
    79381 tcaatatctc tagaaaagtt tgcgaataat ggaatatcaa atcctgcatg caattcaacc
    79441 atgggcctat tgaatttact accttcttca agtaaatcaa attctgaata acttttgtta
    79501 tagcctaatt cagcttttga aacattgcta tgcatttctg atacaaaagg ttgggcaaat
    79561 gcagctgatt caaaaaatcc atcccagcta atagcagtcg tatcctgaga agataaagta
    79621 acaaatggta atgttactaa acaaaatgta atgaatccct tttttatcat ggtagtatct
    79681 tttttattaa agttaattga ataaaaatta aacaaccagt actaaactta aatagttcat
    79741 tcattgttaa tttattttac acattattag gttatttaat atttttacgt agaaaatagt
    79801 tttattggaa tgattgatag tgaagtaatt cagaaaatac ttgaaggcaa acgagattct
    79861 tatcgtattc ttgttgagcg ctatcagtct atggtttatc gcacatgcct aggctttttg
    79921 cacaaccaac atgacgctga agaccttact caggaggttt tcattaaagc atatcaatct
    79981 attgactcat ttaaaggcga ttcagctttc tcaacctgga tataccgcat agctgttaat
    80041 gcttcgttaa acaaagtacg agcaaattca aaaaactttc tgttccaaaa gctggataca
    80101 cttttaggaa gtggcaaaaa ctatgagcca tctgttgttt ttcacgaatc agaaaacccc
    80161 gaggatatgc tggttaaaga agaacatcgc ctgtgggtgc aaaaagcgct ggacagcctg
    80221 cctgaaaacc aacgcacagc tattgtttta agcaaatatg atgaactatc gcaaaaagaa
    80281 attgccgaaa tcatgaatac cacagaagga gctgttgaat cccttataca acgggcaaaa
    80341 acgaatctca gggaaaaatt atttgcccat ttgaaaaaaa ataaaaaatg accgtaggaa
    80401 aaaatgaagt tttgtttcta accataaaaa tcaattaaaa tgaactgtga tttatgcaaa
    80461 cgagaattag aagcttaccg cgaaggcagg ttgcccgacg gtatccgggc tcaggttgag
    80521 catcacctgc ataattgcca tgattgtact ttatactacc atgcactgat agcggctgaa
    80581 aaggtaatgg acgaggaaaa aagcgcgcaa cacaatccat ttctttcaac ccgcatcatg
    80641 gcaggtatcg aagctcttga aagcagccaa aaacgtgttc gtcagggcat actgaagccg
    80701 gttttaatca gtatttctat tgtaatagca gtatttatag gtatcaccat gggacagctt
    80761 tataaacctg ctaaacaaag taatgttatc cctgcagaac tgacatatat ggatgatgca
    80821 agtatggaat cgttaaatgt gtttgtaacc gaataataca agagtgatga aagctgaaaa
    80881 caaacatacc acaatggttt gggcaattgt agtactggca ataatgaata tatctacatt
    80941 tgttaccatc atgtatcatc aacataaggc agaaactatc attcctaatg atgtaaaaac
    81001 accaaaactc gaagctgttg ccgaaaaata cagtggccgc tattttaaag aacagcttgg
    81061 gtttaacagt gaacagatgg acaaatttcg cgagtttaac cgtccgttcc gaatgcaggc
    81121 aagagaaatc acgctacaac tggctgatgt acgaaaagct tttttgcaag agattgctgc
    81181 gcctaacagc gacactgcca agcttaatgc gttgtccgat tctattggcg aaatgcacaa
    81241 gcagttgaaa aagctgatgt tccaatattg tcttgacatc aaaaccatct gtaataatga
    81301 gcaaaaggag aagctcaacc agttgttcca ggatatcttt atcaacgagt cgcctatggc
    81361 acttcccgga cacgggccaa tgaaaggtat gaaaaaaggc atggaacaag ggcagtgcaa
    81421 atcacagtga tttcagaaag ttcatacaat taatggttag atttaaaaaa agaaagaaat
    81481 gaaaacaaaa cttattttat caggaatagc attatttgca gccatttcat tggcaaacgc
    81541 ccagggaaat tgttgcgcaa aagcaaagta caataaggat tccaaaggca caggctacgt
    81601 agatgccaat aaaaatggca tttgcgacaa ctacgaaaac agggccgaca caacttctgt
    81661 tggcaaagga aaaggtaatg gcaaaggaaa aggtaatagc aaaggcaaga actttgtgga
    81721 caacaacaaa aacggcattt gcgacaatta cgaagcccgg aataaaaagt aaatcgggca
    81781 ccttgcacat acctttagta taattaattc attatcaatt tattaaaaaa attattttca
    81841 attgttctga aaagtcagga caattgaaca gggtaaatag tgtcttgtca aaagtaaaaa
    81901 catcaaaatt ttatcatcat gaaaacaaca ttcagtaaaa tagtaacagg tagctttata
    81961 gctatagcag caatgaccat gagttcatgc gaaaaagatt tgtcaaactc agaaaatatg
    82021 cgttatgcaa gcatcataga tgtagcaggc gacggaacaa cctccgttat gcactccaac
    82081 atgcagcaag cctttgtcga aacagctgct ttgactgaga ctgagctcgc ctctcttgtg
    82141 aaaataaaag acgaagaaaa gcttgcacgt gatgtttatg cagcattata ccaaaaatgg
    82201 gcaagtccgg tattctcacg cattaccaat gcagaaaaca accatctttc ggctatcaac
    82261 ttgttattgg cctattatgg ttcctcagac actttgattg gcgaggtagg agtgtttacc
    82321 aatgcagaag ttcaggcatt gtacaacaac ctggtatcag caggctcaac aactattgaa
    82381 gatgcttata aaaccggcgc tttgatagaa gaaatggata tcaacgattt gcaaaacgtt
    82441 atcgctacca cctctaatgc caacattacc atggttatgg aaaatctgga acgaggctca
    82501 cggaaccatt tgcgcgcatt taataaacaa ttagtaactt taggtattac ctatgttcca
    82561 acctatatct cgcaaagcgc ttatgaccag attgtcaatt cttcaataga aaagggcaaa
    82621 aactatatga tgcagggcaa tggcaaaggt catggaaaag gcaagggaaa gatgaggaat
    82681 ggcaatggtg acgggagttg caataattga ttgacctgaa ttagcttgta aaccactcct
    82741 atcctccatt tgtaaagggg aggaattgaa tatctcaagt ttgagcagta tgaggctgtg
    82801 taaaaagtct gaaaaggctt cgacttcgct cagcctgaca attgataatc agcaatttgt
    82861 cattttgagc ggagtcgaaa aatgaacaaa tttgactttt tacacaccct cggggtggta
    82921 aaaaattttc ataacaaaaa aatatctgat ttatgccgaa aataagcaaa atagcagccc
    82981 tgacattcat cctttggata acatccgtta tgtcagtgtt ttcgcaagca aaattcacca
    83041 tcagcggaga aataaaggat tctaaaacag gtgaaacttt aattggtgca actgtatatc
    83101 cttcggagat gccaaccaaa ggagtagtaa ccaatgccta tggtttttat tctatcaccc
    83161 tacccgaagg caaatacaac ctggtatcgc aatatacagg atacgaaaaa agccagcaaa
    83221 caatagacct gaaacaaaac atgaaagtca attttgaact gggcgaaagc gttatctcgt
    83281 taaatgaagt ggtggtaact gctgaaaaac aagaccgtaa tattacgtca acgcaaatgg
    83341 gcatgaacaa gctaaacatc aacgaagtaa aaaatatccc ggtgctgttt ggcgaaaagg
    83401 atgtgctgaa aaccatacag ttgctgccgg gcgtaaaatc agcaggcgaa gggaactccg
    83461 gcttttatgt acgtggcggg agctcagacc agaacctgat tttgcttgat gaagcgccgg
    83521 tatataatcc ttcgcacgtg atgggcttct tttcggtatt caattctgat gccatcaagg
    83581 atttgaacct tatcaaaggc ggaatgcctg ccgaatatgg cggtcgtctc tcttccgtac
    83641 tggatgtcaa gatgaacgaa ggaaacaata aagaatatca tgtaaacggt ggattggggc
    83701 ttatctcatc acatcttacg gttgaaggcc ctatacaaaa agacaaaagc tcatttatga
    83761 tttcaggacg ccgcacttat gccgatatat tcctaaaagc ctcttccaac gagatgctta
    83821 aaaataccaa catttacttt tacgacctga acgccaaaat gaactttcac cttggagaaa
    83881 aggacaggct ttacctttca ggctattttg gtcgtgacga catcaacctg cttcgtttca
    83941 gtaactcgtt ccagtcgatg tggggcaata ccacaggcac gttgcgttgg aaccacattt
    84001 tttccaacaa gcttttcctc aacacttctc ttatatacag cgattttact tacaacatgc
    84061 agcttggcag cgacagcaca caggtaaatg ttgaatcctc tatacgcgat tataacttca
    84121 aacaagactg gcaatactac atgaactccc aacacactgt gaaatttggg tataatgcca
    84181 tttatcattc atttattccg ggcagtgtaa ccggcaatgc tacaacatcg cttgtaagct
    84241 ctgacattcc aaagcgatat gctttagaaa acgctgcata cctgagtgat gaatgggccg
    84301 tttcggaaaa attaaaaata acagcaggtt tgcgtatttc aaactttaac cagcttggcc
    84361 cgaccaccat ctacggattt gatgaagatg gaaccattac agatactacc gtttacaaaa
    84421 ataatgaaaa tatcaaaacg tattggggaa tagaaccgag gctaagcgcc agctttatgc
    84481 ttaacgaaag cagctcgata aaagcttcgt atgcccggac acagcaatat ttgcatctgt
    84541 tatcaaatgc tacctcttat caatccactg atttatggat tccgagcagt aacatcgtaa
    84601 agccacagat cggcgatcaa tacgccattg ggtatttcaa gaactttgcc gacaaccttt
    84661 tcgaaacatc ggtagaagtg tattacaaaa ccatgcaaaa ccagatagaa tacaaaaacg
    84721 gggctcagtt tgtactaaac aaaaccgccg aagctgacct ggttttcgga aaaggcaaat
    84781 cgtatggcat tgagctattt gcaaaaaagc gctatggcaa gttcaatggt tggatcagct
    84841 acacaatgtc ttatactgac aggacttttg cagacatcga tgctggcaaa acatttgctg
    84901 ccaagcagga cagacgacac gaagtttctg ttgttggtat ttaccagtta aacccacgct
    84961 ggtcactttc ggcaacgtgg atttattata caggaaatgc cgttaccttt cccagtggaa
    85021 agtatatggc tgataaccgc attgtatatt actacaccga gcgaaatggc tatcgtatgc
    85081 ccgattacca ccgtatggat gttgcagcaa catggattca caaaaagact gccaaatttg
    85141 agtcgtcatt taacttttcc atatataacg tgtataacag gctaaacgct tactccatct
    85201 cattccgcca aagcgaaacc aatcctgacc aaaccgaagc agttaaactt tcgcttttcc
    85261 ctattattcc ttcagttaca tataccttca aattctgaaa ctatgaaaat gaacactata
    85321 tacaaattag ctttagctat tccggttatt gcagcatttt ctgcctgtga ggaaattatc
    85381 aatatcgacc tgaacacggc ttctccaaag ctttcggtaa cagccatcat caccgaccag
    85441 cccgggccat attacgtgac ccttactaag tcagaaagct tctttagttc caacgattca
    85501 ttcccggcag taagtaatgc tgtggtggtt ataagcgatg atgccggaaa ctcagaacaa
    85561 ctaacggaaa tcaaagccgg ggtttaccaa accaataatt tacaaggtgt tagtggtaga
    85621 acttatcatc tatccattga agctgatggt aaaaaatacg aagcatcatc attcctgccc
    85681 tatcctgtag ccctcgactc ggttgcatct cagaaaataa cagccacaag ccccggtaac
    85741 aaggaaaaca aaaataaata ttatctcaag tgttatttta ctgaccctaa aggaagcgaa
    85801 gattattatc gtgtagaacc aattattcca aatctggaca ccttgtcttc cagctaccag
    85861 atatacagcg acgaggttac cgacggtcaa catgttgaat ttccagtaca aaagccaaca
    85921 ttcagctttg gagatactgc cattgttgag ttttatcatg tcaacaaaga aaattacgat
    85981 tattacaaaa ctgctaacaa tatcctgaaa agcaaaaaag gcccaatggc ctctgcttct
    86041 gcaccacagg ccaacccgtt aaccaacatt tcaggcgaag ctgtgggcta ttttggaacg
    86101 tttgcggtca acagaagact tattgtggtg aaataataaa tacatgaata ttcatcaatt
    86161 gaaaacttga tgaatattca tgctgccaca tacaggaaaa acctcaaaaa ggtcattacc
    86221 catgcatgtt tcagtttttt cccagctcaa ttcttcttta gggccttgcc aattgcagtc
    86281 tttacaaatg taaatactat gccatcgcag caattatttc atctacagtt ttaaatgaat
    86341 tgggcaacaa atcaggaggg cacttaccgg ctttttcttt taccaggtat cttttacatt
    86401 tcatccatcc atcttcgcct gcatcgcaat attgcttgcg ataattgctt gctgtagcat
    86461 cttttccttc taaaacaccc gtataaatag ggcatttttc aaaatttttg catgtagttt
    86521 ccataaataa agtttgagag ttatttaaaa tgtaacaagc tttttgctcc tttttactat
    86581 ttcgtacaaa tcgtatatgg aagtaatggt acacatttgc acattctcag tatggcgcat
    86641 ggcagcacat gtagcgcaag tgtaaatatc tccccccatt ttcaaaaatt cgaggctggt
    86701 tttaggaata tcatacttgg aagtatcttt cataaataca tctacagcct tgctaataac
    86761 gaaaaccacc accgaatcgc cttttgactc ggctgcaatg ccaaggcgca aagcattcca
    86821 aaccgtttcg gcatcgcccg aactaataac tatgccaata tctggcttcc gagatttgtc
    86881 aaatgccgaa acgttttcgc aagcagaaaa taactgcgaa aagccattag taaaagaaat
    86941 ggccagaaca aggaaactaa aaacggtttt tttcatccgt aaataatttt ttaataaggt
    87001 cggatggaac tcgcatgatg aattatcatc cttgtttgtg tttaacgaat catgctcgtt
    87061 tcattttatt atcacatcga attcagtaaa aataa
//
LOCUS       DAOSPF010000004        81325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_301_length_81325_cov_7.289898, whole genome shotgun sequence.
ACCESSION   DAOSPF010000004 DAOSPF010000000
VERSION     DAOSPF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 81325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_81325_cov_7.289898"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="PK252_01390"
     CDS             complement(<1..1838)
                     /locus_tag="PK252_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOT13389.1"
                     /translation="MKQFKLFNSIIAWVTFAIATAVYLLTMEPTASFWDCGEFISTAF
                     KLEVGHPPGAYLFMIIGRFFTLFAGGDVTKVAIWMNSWSALASGLTILFLFWTITHLG
                     RKLLIKDGNYTTGKTIAVLGSGLVGALAFTFSDSFWFSAVEAEVYASSSLFTALVFWA
                     VLKWEDQADDKHANRWLILIAYLMGLSIGVHLLNLLAIPAIVFVYYFKKYPVTTKGII
                     ITSLVSIAILAGVMYGVITGLIVLASKFELLFVNGFGLPYFSGVFFYLVALVVLLAWG
                     LWYTYKKRKVVLNTALLMLSVILIGYSSVAMVVIRSSANTPLDENDPENMFSLLSYLN
                     REQYGDRPLFSGPYYNTPILGMEENGKPTYTPINGRYEITNHKPEYKYDERFLTIFPR
                     MYSSEQSHQSAYEYWGKVKGVPIQTQNQKGEPTTIMKPTFSENLLFFFRYQVNFMYIR
                     YFMWNFVGRQNDIQGAGEPTKGNWISGIKFIDEIFLGPQDKLPDSMKNNKGRNTYFFL
                     PLILGIIGLWYQLKKDKKDFSVVMLLFFFTGLAIVLYLNQTPYQPRERDYAYTGSFYA
                     FAIWIGIGVAALYEMLSDKIPSVLKAGGITAICLLCVPVLMGQQNW"
     gene            complement(2256..2654)
                     /locus_tag="PK252_01395"
     CDS             complement(2256..2654)
                     /locus_tag="PK252_01395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOT13390.1"
                     /translation="MATIFSRIIAGEIPSYKVAEDDNFFAFLDINPLIKGHTLVVPKK
                     EVDYLFDIDDALLEKMMVFSKRTAKAIEKVVKCQRVGLTVIGLEVPHAHIHLIPIITG
                     MQDMNFANPKLKLTKEEFEEIALAINKAWR"
     gene            complement(2954..4057)
                     /gene="ychF"
                     /locus_tag="PK252_01400"
     CDS             complement(2954..4057)
                     /gene="ychF"
                     /locus_tag="PK252_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT13391.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLTKLAEIDKPARIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAIIH
                     VLRCFDDENVTHVDGSINPVRDKEIIDMELQLKDLETVESRIAKVQKQAQTGGDKQAK
                     RLLEILLQYKEVLLHGQSARVVKLDNKEDQKLANDLFLLTDKPVLYVCNVDEKAVIKG
                     NKYVDMVKEAVKNENAEVLIVAAKTESEIAEFDTYEERKMFLDELGLEESGVARLIKS
                     AYKLLNLETFFTTGADETRAWTYIRGTKAPQAAGVIHSDFEKGFIRAEVIKYNDYVTL
                     GSEAACKEAGKLSIEGKEYVVQDGDIMHFRFNV"
     gene            complement(4234..4716)
                     /locus_tag="PK252_01405"
     CDS             complement(4234..4716)
                     /locus_tag="PK252_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13392.1"
                     /translation="MKKKTLILPCTMDDFNEITRDIVDFWGSDRTLYLHHPYLVYEFG
                     NTAFVIKEKGQVIAYLFGFFSQTESVAYVHLLGVRESCQRQGYGQMLYEHFINIAKQK
                     GIKKLKAITTPTNFKSVSFHKNKIGMTLLGQPDSNGINVVKNYSGIDKDRVVFLKEIK
                     "
     gene            complement(4728..5057)
                     /locus_tag="PK252_01410"
     CDS             complement(4728..5057)
                     /locus_tag="PK252_01410"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOT13393.1"
                     /translation="MTDSFFQKVYNVTKLVPHGRVTTYGAIARFIGSPQSSRMVGWAL
                     NNCHHLEEFIPAHRVVNRNGMLSGKHHFGNPSIMQQLLENEGVEITNDHIVNFDKLFW
                     NPSVELK"
     gene            complement(5026..5754)
                     /gene="trmB"
                     /locus_tag="PK252_01415"
     CDS             complement(5026..5754)
                     /gene="trmB"
                     /locus_tag="PK252_01415"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOT13394.1"
                     /translation="MGKDKLRRFAELHTFQHVIEADYNQVYQKDHAVKGTWHKDIFRN
                     NNPIILELGCGKGEYVVALAQIYPDKNFIGIDIKGNRMWVGAKQALEKGLTNVYFVRT
                     RIEFIESFFSKDEVDEIWVTFPDPQMQSNRRRKRLTAPSFLNRYKNFLKPQGIIHLKT
                     DSLFLHKYTKAVIEHNQLPLKVATDDLYNSPYSQLTYGVTTHYEKLFIQKTKKITYLQ
                     FQLLQQEIIDIDQTYDRQLLSESI"
     gene            complement(5767..6234)
                     /locus_tag="PK252_01420"
     CDS             complement(5767..6234)
                     /locus_tag="PK252_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOT13395.1"
                     /translation="MKTKLSLLVLVVLTVLSCVNREQYVQNFSIADSKWKISNKCRFE
                     YINNDTVSPSNVYFNLRHSGNYKYSNIYLFVTTMAPNGNKIIDTLEYNLADAKGRWIG
                     KGLSDIKNIKLGYKKAVRFSQNGKYMFYVTHGMRENELEGIIDVGIIIEKSKQ"
     gene            complement(6218..7510)
                     /gene="ricT"
                     /locus_tag="PK252_01425"
     CDS             complement(6218..7510)
                     /gene="ricT"
                     /locus_tag="PK252_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOT13396.1"
                     /translation="MQKDIILRGCSEVVNDNMETFKCNPSPCSKLNTYNWAYDLPQGL
                     IPYDVVEVRFKNTRKGFYRNVNGLEIKQGDIVAVEASPGHDIGIVSLVGEQVRQQIKR
                     YKVRTENGDYRKLYRKAKQTDIDKWYESISLEESTMLKARKISDSLNLNMKIGDVEYQ
                     GDRSKAIFYYIADERVDFRELIKVLAEEFRVRIEMKQIGARQEAGRIGGIGPCGRELC
                     CSTWINNFVSVSTNAARYQDLSLNPQKLAGQCGKLKCCLNYELDAYLDAQKDFPDTNI
                     VLETAEGPAYHQKTDVFRRVMWYALNKNEQTNFIALSVEKVFETIESNKKGIKVKLDS
                     EELKPQGAVPVIEELDYANVVGQDSLTRFDDKKKKDKQQFQKNRPNKPNSANNRQNNP
                     ENANRPNNPGGANRPKFQNNPNRQNKQNNQQKPNENKA"
     gene            complement(7725..8735)
                     /locus_tag="PK252_01430"
     CDS             complement(7725..8735)
                     /locus_tag="PK252_01430"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT13397.1"
                     /translation="MKKTILISALFILSIFSINAQSYKAVKADVAPVVDANGSDACWE
                     KAPWAKIDQTWIGSKTNDADFSGKYKVVWTPEQLYFLVEITDDKHYPKYPGNCNNIHN
                     FDCIEIFLDEDRSGGDHQYNFNAFAYHIAANGDVCDLGTDRVNHLFKNDVVSVYDTIS
                     ENVYLWEVGIKVFDNTYVYGGNNTPVTLGLNKIMGMSVAYNDNDNGSTRESMFGSVAI
                     AGADKNVSWINASYFGSVILLNDSNQTDVKSSITSGDNLRCSWEPASKSINISLVNSN
                     TGTWKAEVLDITGKVVIAKSIYKSGSLATDEIKVPNSRQGLYFVRMSNGGAQFCSKVI
                     VK"
     gene            8978..9907
                     /locus_tag="PK252_01435"
     CDS             8978..9907
                     /locus_tag="PK252_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT13398.1"
                     /translation="MDIKVEKLTKNYGHHKAVDDISFEVKTGEILGFLGPNGAGKTTT
                     MKIITNYLNCEQGDIIIGGKSIRDNAEELKKHIGYLPENNPLYLDMPVIDYLEFCASL
                     QGVEKSKIPERIREMVRVCGLNAEKHKRIGELSKGFRQRVGLAQAMIHNPSVLILDEP
                     TTGLDPNQIIEIRKLIRELGREKTVILSTHILSEVEATCDRIMIINNGKIVANGTAET
                     LRKQAQGQEILTVKIEDGDSNAIFKAIQELPTVGLVDFTDRNLNRFEVQSRPGCTSRR
                     EIFNLCVKNGWILTELAVSEAKLEDIFRELTVN"
     gene            9948..10673
                     /locus_tag="PK252_01440"
     CDS             9948..10673
                     /locus_tag="PK252_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT13399.1"
                     /translation="MNSVWIITKKELNSFFDSLIAYILLVLFLGFSGFFTWMFGSDIF
                     LIGQATLRPFFSIAYWSLFIFIPALTMRMIAEERKTGTIELLLTKAVSDRQLVLGKFL
                     AAFLLICISLALTLPYVITLASIGNVDNGEVICGYLGLALMSGVYIAIGLYASSLTNN
                     QIVAFLSALFIGLFFHIIFDILANNFKGSIGEFFNMLSLSNHFESIQRGVLDTKDLVY
                     FLSLIFLGLFLTENNLAKRKVNG"
     gene            10800..12326
                     /locus_tag="PK252_01445"
     CDS             10800..12326
                     /locus_tag="PK252_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT13400.1"
                     /translation="MMKNKMKNKWITSVVLITAIVLVANLISQDFFLRVDFSEDKQYT
                     LSWATKDLLKNLHEPITVKAYFSENVPPNVAKVRKDFKEMLVEYNNRSKGMVVYEFVD
                     PSAKEDIEQEATQEGIQPVMIDVREKDQMKQQKAYLGAIISMGDRKEVIPVIQPGAAL
                     EYTLSKAIKKLSVVEKPSVGILQGHGEPQIQELAQVYAELSVLYQVEPLTLNDSAAIP
                     ERIKTIAIVRPTDSIKQSHFAQLDAFLARGGKILVAASNVNANLQQAIASASAAGIDQ
                     WLKTKGILLNQNIVIDASCSQIQVVQKNGAFQMIQQIQFPYIPVIKTFSKTSIGSGLE
                     AVILPFASTIEYNGSPANIYTPLAYTSDKSNIEQLPVFFNIQRIWHESDFPKHQLVVA
                     ATLEGKLAGDQRSKLVIIGDGDFVINGKQGEQQKLSPDNINMFVNSIDWLSDDTGLIE
                     LRTKSVTARPLDELGDGTRTTLKWLNFLLPILLIGAYGIWRAQAKRIQRIKRMEERYV
                     "
     gene            12319..13299
                     /locus_tag="PK252_01450"
     CDS             12319..13299
                     /locus_tag="PK252_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT13401.1"
                     /translation="MSNKINNKTLLIVLAGLIIVFVLVKWYQKTQTESTLNTALVKID
                     TTKVSRIAIYPVAENRQEILFSKIGKDWKVSKGNITAEVDENVINNLIITLSEIKVKS
                     LVAKNKDKWDDFKVSDTTATRVKVFEGNDVVADLMIGRFTYQQSQNPYGGMYGGGVIG
                     STYVRPADEDEVYAVDGFLTFTFNQQFTAFRKQTLARFETNKIEKITFKYPADSSYTL
                     ELKDKKWFIGDKMADSAKVASYISALSYKNASSFVDNQSALNAPAWLVRVEGKDMQPV
                     SIEASSDGSKFIINSTQNPKSYFESAKNKLFDEIFKPQSSFFATKEKKKK"
     gene            complement(13355..14194)
                     /gene="panC"
                     /locus_tag="PK252_01455"
     CDS             complement(13355..14194)
                     /gene="panC"
                     /locus_tag="PK252_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT13402.1"
                     /translation="MQIIKSSKELHSQMLQYREEGKTIGFVPTMGALHEGHISLVSQC
                     RSENDVVVVSIFVNPTQFNDPKDLERYPRTLEADTAMLEKAKCDIIFHPEVNEMYPEK
                     DTRVFEFGQLDKVMEGKHRPGHFNGVAQVVTKLFDIVVPHKAYFGEKDFQQLAIIQYF
                     VKQLKYDIQIIPCPIVREPDGLAMSSRNKLLTPEHRAAAPLISKIIFESKKLKGSKKV
                     DELKQWVIGQINNNELLKVEYFELSDRYTLQPIKNWEDSKDVVGCIAVFAGKIRLIDN
                     VLY"
     gene            complement(14377..14913)
                     /gene="hpt"
                     /locus_tag="PK252_01460"
     CDS             complement(14377..14913)
                     /gene="hpt"
                     /locus_tag="PK252_01460"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT13403.1"
                     /translation="MQSISIDNKEFIPYISKDKITLLVEHVAQKINNELCNEDVVFIG
                     VLNGSFIFMADLIREINFHVTISFIKLASYQADKSSGEVKQIIGLNIDLKGKTVVIVE
                     DIIDTGITLEYLLKQIEQQQPAKIKVASMFFKPKACKMPVHIDYLGTEIPNDFVIGYG
                     LDYNGFGRNLPDLYRLKS"
     gene            15087..16028
                     /locus_tag="PK252_01465"
     CDS             15087..16028
                     /locus_tag="PK252_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOT13404.1"
                     /translation="MKTVFSEIFKGLFHKALSTAWWMIKIMLPVSFVVMLMQYFGWLE
                     VIASVFKPVFSFIGVGGEGALVFITSILLNIYAAIAVIGTIGFGIREITILAIMCLIA
                     HNLIIETAIQKKTGSQAARMVILRISMAIIAGILFNWLLPASTYVQHIVTKAGQHLTL
                     TQVMKNWVFDSVFLSFKVIGIIIGLMFIQRFLEYMGIMQKLSAAFRPLMKPFGLSENT
                     SFHWLIANVVGLTYGSAIMIEQVESGKLKLRDADLLNHHVGISHSLLEDTLLFVAIGV
                     SAWWIVFPRLILAMFAVWIYKAELFFLQKKNEISSVK"
     gene            16036..16830
                     /locus_tag="PK252_01470"
     CDS             16036..16830
                     /locus_tag="PK252_01470"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT13405.1"
                     /translation="MKQYLGLLQHVLDNGTKKDDRTGTGTISIFGYQMRFDLGEGFPL
                     LTTKKLHTRSIIYELLWFLRGETNIRFLKDNNVSIWDEWADKNGDLGPVYGKQWRSWQ
                     TADGRTIDQISQLIDMIKTNPDSRRLIVSAWNPGDIDKMALPPCHTMFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVCNLKPGEFVHTFGDAHIYLNHLEQVKL
                     QLSREPRPLPTMKIVRNVNSIFDFKYEDFLLEGYDPHPHIKGEVAV"
     gene            complement(17017..19566)
                     /locus_tag="PK252_01475"
     CDS             complement(17017..19566)
                     /locus_tag="PK252_01475"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOT13406.1"
                     /translation="MSKTPKQTKVPGTFTYKEAFESSVAYFKGDEMAAQVWINKYALK
                     DSFGNIYELNPDHMHRRIAKEIARIEAKYANPMSEEEVYEYLKDFKYIVPQGSPMTGI
                     GNNFQIASLSNCFVIGNSADSYGGIMKTDQEQVQLMKRRGGVGHDLSHVRPSGSPVLN
                     SALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISSKHPDAEKFIDAKLEQGKVTGA
                     NVSVKIDDDFMRAVIDGTEYIQQYPVDSASPSFTKKVDARAIWKKIVHNAWKSAEPGI
                     LFWDTIIRESVPDCYSQFGYKTVSTNPCGEIPLCPYDSCRLLAINLYGYVENPFTPQA
                     RFNFDLFKRHVAVAQRFMDDIIDLEIEKIDAILRKIDADPEDFEVKRVEKKLWESIRI
                     KTEEGRRTGVGITAEGDMLAALGIRYGSDNAIDFSVEVHRTLALEAYRSSVNLAKERG
                     PFSIYDSQLEKNNPFINRLRKEDEKLYQDMVRFGRRNIALLTIAPTGTTSMMTQTTSG
                     IEPVFLPVYKRRRKVNPNDTNVKIVFTDEVGDHWEEYVVFHHKFLTWLEVNGYNVEEV
                     KKYDQEQLNELVAKSPYYKSTSADVDWLQKVHMQGEIQKWVDHSISVTINLPENVDEQ
                     LVSDLYIKAWQSGCKGCTVYRENSRAGVLISNNAKKEKKESFSEPIKRPKVLDAEIVR
                     FKNNNENWIAFVGILDGQPYEIFTGLQDEDIFPIPKSITKGKIIKNKDEDGNTRYDFQ
                     YTDKYGYKNTLGGLSHQFNKEYWNYAKLISSVLRYGMPIPELVNLVSSLQLDSETINT
                     WKNGVERALKKFIPDGTQAKAGQKCGACGSESLVYQEGCLTCKNCGNSKCG"
     gene            20048..21070
                     /locus_tag="PK252_01480"
     CDS             20048..21070
                     /locus_tag="PK252_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13407.1"
                     /translation="MKSLLFLVFALGCNLILGQKVIFLHHSTGGGVYNEGSVAQWFDT
                     YNSQNSTNYEITERAYPDSPYPWENYPYDYWNLWINGACNNDNSGTECINSIVTKYDV
                     IIFKHCFPGAGIGEDEAENSVSSSYKSMGNYKLQYRALRTLMDSFPNNKFIVWTLAPL
                     HRNATDSASAARAKQFVDWVKYQWLKEDSKSHPNIFVFDFWGYVAETNANPSQGKVNC
                     LKYDYEGSHDGDDSHPNTQANQTVGPIFGQFIVDCIKQTNTRIINKTLDNITVFYNNG
                     CLAIQGLNEIVPQSDLCIYSIDGHLLYKKRIQSSIALPLSEGMYLVSIHNKTFGTQTA
                     KFCVIK"
     gene            21167..22531
                     /locus_tag="PK252_01485"
     CDS             21167..22531
                     /locus_tag="PK252_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT13408.1"
                     /translation="MRVHFIAIGGSAMHSLALALHEKGYIVTGSDDDFFEPSKSKLEK
                     AGLLPKEKGWDASRVTPELDAVILGMHARDNNPELAKARELGLKIFSYPEYLYEQTKN
                     KTRVVIGGSHGKTTITSMIIHVLSYWKKSFDYMVGAQLEGYNNMVRLSEDSPVAVFEG
                     DEYLSSTLDPRPKFHIYQPNIAVINGIAWDHINVFSTFDVYVEQFKIFIEKIQSKGTL
                     IYCADDPLVAGIAMTAAPDVKKVPYKIHEHQVISNEVFLINDKNKTRVHVFGKHNMQN
                     LSAAKAVCNELGLTNEQFYEAIATFKGAAKRLQLLSENNMTSIYLDFAHSPSKLTATV
                     RAVKEHYPERALVACMELHTFSSLSEDFLRQYYGTMERADYAIVYYSMKALAAKGLKP
                     LDPEDVAKAFATKHLKVFTDSDQLVKHLKSIEWKGKNLLLMSSGNFDGINFNTFAKEL
                     LNQK"
     gene            22565..23005
                     /locus_tag="PK252_01490"
     CDS             22565..23005
                     /locus_tag="PK252_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13409.1"
                     /translation="MRKLVFLFFIIFIAFTNIKAQQAIGARLSDNFGITYKYTYSNPV
                     KLEGILEIHRHALTVNGLYERYYQTPFSKYFDWYFGGGAFINFWSVNDNGGVNVGLRG
                     VAGICYTFSDIPIDISLDWMPAIQIVSDYHADFAILGLSVRYVF"
     gene            23340..25688
                     /locus_tag="PK252_01495"
     CDS             23340..25688
                     /locus_tag="PK252_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT13410.1"
                     /translation="MRISALILLCFVAQFGYAQKRISGFLTDANGVPLENASIFLKGG
                     RLLTTTDKNGAFSFEYKQDESICFSHFGFSDTCLNASELSENSIKIRLNEKKIVAEEI
                     IVSSKSNMKIIRSAQTGLFEIRTDSLRSLPNIVGEADPIKMLQLSPGVTKSDFNMGLN
                     VRGSTTDQNLVLIDDAAIYNPTHLAGFLSVFNPLVVEKVSLLKSGIPAEYGGRVSSIT
                     IVETDKNLASKTETEGNVGILLSGFVVKTPLLQDKASFQVSARRSYFDQTIKPISKIL
                     FKNGKSMFNSTDYAFYDVNASLIIAPSKNDKVFISCYVGNDDFSLSKTSFNLQYNMQW
                     SNQAFSLKWMHHFSSRHIMKTIAYSSKGDFAIFLGQNDFNFRLNSSIEDLSAIHEHTF
                     FFEHWSLKTGAQVLKQHILPNKSKAELIDFNTDFGTPNTFNTLTFSPYIQSEVNVSKS
                     LALNIGLRYNEYLHFGPFERFVRDNGIDITDTLYYKKNETVKKYNNFEPRFALRYLIN
                     SYSSLKFSVTHNIQYLQQINVSAVSFPTDFWMPASYQLAPQKGWQFSLGYFSNLFDSS
                     YCFSTEVFYKHMVNLTEFKKGILSTVSKASMEENIITGEGRAYGIEFLIEKKTGELTG
                     WISYTLSRSERIFKEINNGNPYPSKYDRTHDLSIVTQYKINEKWNASAIFVIASGNAF
                     TLPYGRYMIGENIVNQYSDYNSFRMPVYHRLDMSVTRKFKTKGKAEHSLDIGIYNVYS
                     RLNPYFMYFDVSGDLEKYKLKVSPRYVSVFPILPSVSYHFKF"
     gene            25701..26633
                     /locus_tag="PK252_01500"
     CDS             25701..26633
                     /locus_tag="PK252_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOT13411.1"
                     /translation="MSIEMSIQEIKPLDKLRSVLKTSFIKSMTIAMCCLTCIACEPEM
                     DYELTSVKKQLVVDGWIENNNYAQVVLTYNTGYFSNLDSASFRGLVASRAKVILSDGT
                     QSEILTLTKDTNYFPPYVYKGNEILGQPGKTYTLIIIDEINTGTREMDTIVAITTIPQ
                     PVKLDSVWFSETSDSSGIIKGIIFDNPDEKNYYRTFSKIKGKDKRFIPTYIASFEDKY
                     FNGQKFTFHLNKGLDSYIKPLYNIEFNKGDTINLKIASIDRISFLFWSSYDQEIFNSG
                     NPFASNFDRVNSNIHGGLGIWCGYGATTYLVVAK"
     gene            complement(26682..28112)
                     /locus_tag="PK252_01505"
     CDS             complement(26682..28112)
                     /locus_tag="PK252_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13412.1"
                     /translation="MKIKILFLAMCASIISSCSKDDDKTTEEVNLEKPVYAEKTVAET
                     KVDMENTGIKMVNELKAMNQDQGVKATVNLLELMNIGTGVNGSIKNSAYFQVMAAVKN
                     ISSNNPDANVIFKSLKAETSNSLELEAEFDKIAGVYTYNYTKNDFDFAANASSGQVVF
                     KFPASIASKKAKNNDGKLVISKPKFVTGNFTLVDTKEMPSELKYELSVDGTVVSSFSF
                     TASYTDEGLPSNVEGKLTLGAFELKLTYGYKETEVTVNYSFTHGSTTILDLGGTAGGK
                     FTKAAIDNAQETKTIKDTSSYTYYNYETGQYETIQNIYEYKETETFPERVLYNANAHF
                     QFMDMKIVGQIDFANFMKEYRKIEENKYSAYIDSAVSMINKNMALVVVYASDNKAVAK
                     AEAYAKNEKYDEYNCTWDANTWEYNCSTVQKTRKVIDVQMVFADGSKSSMETYFNQGF
                     DNLITSFNSFIDDLNKTYNWKINHIN"
     gene            28346..28759
                     /locus_tag="PK252_01510"
     CDS             28346..28759
                     /locus_tag="PK252_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT13413.1"
                     /translation="MKKLTLFIAIAITCFSVKAQYSQDVILSSILKTDTTSIGQKIIF
                     PQNNNNEISIIKVTIPPGKSTGWHKHSNPVFAYVISGTLTVELEGDKKLTFSANTSFA
                     EVINTYHNGTNLGKEEVVLIAFFMGEKGKLLSEHK"
     gene            complement(28769..29758)
                     /locus_tag="PK252_01515"
     CDS             complement(28769..29758)
                     /locus_tag="PK252_01515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT13414.1"
                     /translation="MNKRILGKTGFNISEISLGTWQVGGKWGDKFSHENADRILNLAI
                     DNGINFIDTADVYGDGESEKAVGRVVRSRKERVFVATKCGRKLSPHVDAAYQPAVLRK
                     FVEDSLKNTGLEAIDLIQLHCPPTETYHRPEIFALFDELKKEGKILNMGVSVERVEEA
                     LKAIEYDNVTTVQIIFNMFRQRPAKDFFAEAAKKNVGIIVRVPLASGLLTGKYSKATV
                     FGKDDHRTFNRNGEAFDKGETFSGINYEKGLAAVDELKKIFPANADLALIALRWIIMF
                     REVSCVIPGASKPEQVLSNLKACELPNLDQEQLQAIEKVYNQYILPDLLKEKW"
     gene            30044..32107
                     /locus_tag="PK252_01520"
     CDS             30044..32107
                     /locus_tag="PK252_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT13415.1"
                     /translation="MKRNICMKAFYSIVQKIVFGAIAFTVLSINQEAKSQNIKQFQGA
                     YENGTATYQYYENANYERIYQGSFKYKGIVMDGLKGKLNLTVVGQYNNNKKDGLWTYT
                     LADPSPTGVTEVVTGNYSNGNMEGEWSSTTKVNNTKKVFKSTSAIFKNNKLFGEINYT
                     YKVYTFKDYAAISIKGSFNDSSFYNGTWLTTYTKGNIQYEETRKYNNGVLCLLVHRRL
                     SDGQIIERYDSTAFVDKFFMNYNAAHNVGMVNDQKYVLQNVDKPSGSLDLFSIVVNYW
                     TNNKNTAYYNTLNAPNPMFVISHGYSPAVISREKTIISWLKTREGERQQWQSEQDKKL
                     LQEKYAAQLAIADTAFNRAKYDEAIAAYKVALTIKDEQYPKDQIHKAGLKLEEIRLRM
                     EMEQKAKELEQQAREKSYKELLTKADAAFAEKRYDVAIELYQSALAIKVDDYPAKQIK
                     AINDIKNDELKAKLIKELDKLWVTVEAGTFKMGCIRSDMNCLRNEEPVHEVYVNTFQM
                     SKYEVTIGQYRMYCKATGKPEPLGADSLPANNISWYEAKEFAEWAGCRLPTEAEWEYA
                     ARGGNKNKKTLYSGSNYIDEVAWFSENSNSSAHPVGKLKPNVLGIYDMTGNVWEWCFD
                     WLSDYSETSDINPTGRSTGTRKVKRGGSFSETNFESDLRVTYRGSEPADTRLYNLGIR
                     LVKKQ"
     gene            complement(32373..32945)
                     /locus_tag="PK252_01525"
     CDS             complement(32373..32945)
                     /locus_tag="PK252_01525"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT13416.1"
                     /translation="MTGFYISIGSILIFLMIENFLRFSKDAKSLKTSDSDKGTTAILR
                     FALISTIILVLCSYFLNLYNILQIDSQVLFFIGLSFVIVGLIIRLIATFTLKEYYTRT
                     LKITDKHKIVKHGIYSIVRHPGYLGVTTFLVGAGLLTGNLISIILIVLTVPFSFIKRL
                     LIEEQMLLKHFGNEYKEYMEKTYRLIPFIF"
     gene            complement(33243..34007)
                     /locus_tag="PK252_01530"
     CDS             complement(33243..34007)
                     /locus_tag="PK252_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT13417.1"
                     /translation="MPVKISIITAVYNSEKTLEEALNSIALQTYTNIEHIVIDGGSTD
                     SSKDITERHLDKIAVYISEPDKGIYDALNKGISKATGDVIGILHADDTFDNNEVLADV
                     ANAFEKEQCDVLYGNLLYVSFEGKIIRDWKSLPFDRKMLSRGWMPPHPTFFVKREIFA
                     RIGNYNLQYRIASDYDLMLRTLLLPGIRIYYYPQIITRMKVGGASNRSLKNIIRKSTE
                     DYHIMKRNKVGGCFTLFMKNFSKVGQFFLRKSKEIE"
     gene            complement(34007..34552)
                     /locus_tag="PK252_01535"
     CDS             complement(34007..34552)
                     /locus_tag="PK252_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HOT13418.1"
                     /translation="MFHTRLDKYNNSWYHPGRNKLVIALWYIVNAVFFCSFITLPYSI
                     KNAILRLFGAKVGKGVCIKPRVNIKYPWRLSIGDYTWVGENAWIDNLDQVNLGKNVCI
                     SQGALLLCGNHNYKKETFDLMIAPINIEDGAWIGAKAVVCPGVHVASHAVLTVGSIAT
                     KNLEPYSIHSGNPAVKIKNRI"
     gene            complement(34560..36884)
                     /locus_tag="PK252_01540"
     CDS             complement(34560..36884)
                     /locus_tag="PK252_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT13419.1"
                     /translation="MQSHNTNDENTRLLVERNAILDSIPFAAWLKDKDGKFIYANTIY
                     EDIAGISKANMVGKTVFDFLPYEIANTFHQEDLKVIEKKQTIHFQTRRENGWYATVKS
                     PIINSEGEVIGTFGFERNISDNIETLNSLKHERDLMHSLMDNSPDLIYFKDTSGRYVR
                     INKAKALNMGLDETQMAHGKTDADFYSEAYAKEQDIDDKKVIETGQSILGKEELLTQP
                     DGTSRWYSTSKSPIFNENGKIEGLIGISRDISEKRKVNSMLEQERNFLQVLMDNIPYT
                     IYLKDPQGHFTKINKAQAKLLGVEDANEAIGKTDFDFFPQTVAETAFQDEQELLNSGK
                     PMISKLELLKDAENNTIWVEATKIPVKDREGNIIGIVGMSIDITEKRLAEERLRKAKE
                     KAEESDMLKTAFLANMSHEIRTPMNGIIGFSNLLRNPDLTDIEREEFLNHITSCGNTL
                     LNLIDDIIDISKIEAGQIKIRMLDCNLNNLMDEIFESFEASRIKENKNEIKLICTKSL
                     PNEDCNIVTDPFRLRQIISNLIGNALKFTFKGSIEFGYEFLENKTIQFHVKDTGIGIP
                     KDKQKIIFERFGQVIDSNFFINQKGTGLGLAISSNLTRLLGGSMWVDSEPGQGACFYF
                     NIPYFAGKSSEKPLPNSIHNLNITNIAFPKDKVILIAEDEDYNYLYLSYILNGLTENI
                     IWAKNGQEAVDAVRNNANIAIVLIDCKMPVKDGYTAMKEIKSLRPELPVIAQTAFAMA
                     DEMEKAIEAGCDGYITKPIQIDVLLGTLNNFINK"
     gene            complement(37047..38015)
                     /locus_tag="PK252_01545"
     CDS             complement(37047..38015)
                     /locus_tag="PK252_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT13420.1"
                     /translation="MENIIEVVAGIDIGGTNTKFGLVDKKGNCYCEGSVPTNSDRNIE
                     NFVNELSKLIKENLTSQPDLQLKGIGIGVPNGNYYKGTIEHAANLKWKGIVPLAKLFK
                     SHFEVPVALTNDANAAAIGEMVYGGARFMKDFIVITLGTGLGSGLVSNGKLIYGHDGF
                     AGELGHTIVRHNGRQCGCGRRGCLETYASATGIRRTVFEILSESMENSPLRDISFNEL
                     TSDKIAEAATKGDVIAKEAFEYTGELLGQKLADSVAHTSPEAIFLFGGLAKAGDLILK
                     PTQQSLEKHLLPIYRNKIKVLLSEMGEINAAILGAGALIWKEIEQN"
     gene            complement(38169..39662)
                     /locus_tag="PK252_01550"
     CDS             complement(38169..39662)
                     /locus_tag="PK252_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13421.1"
                     /translation="MLGVCCQNIDMQGQAVLPLNNDYWLSYENGAVSNNQTSYTFIRP
                     FVFDTCKKDIDEKVLFSGYDAKPHKRYAGKKLFDESIVKYDTVGVKIYADPLFDFALS
                     REINTNENYTTNTRGVRVWGNVNSSLFFEANVYESQATFLPYLYERFKATNAVSGQGR
                     ARKYDTGFDYGLASGRIAWFVSKKLLFSIGTDKMFVGNGYRSLLLTDNAFNYPYALYT
                     LSVGKFKYTQSLLVLMGDSIPKTSFGVWERKLSSMHILSFLASQDIHLSVFEYNIYHY
                     PNKRSNIDFAWEAVNPVMFVNTASQNEKSSSLLTFDMKVNIFNTLTCYGQLAASNIKD
                     NGFKAANTGWQGGVKYYNAFTLRNLVLQYEHNKAGNKLGLNEDTLLNYTHHMQSLAHA
                     WGNNFSENIIIAAYRHKRIFVNALYNWGSYGNKNYGEKEIKVLPGQSAYNDLKYFTLS
                     LSYVLNPYTNRRLELGIMKREASYQNRSNLFFYFTFKTNLINKYYDF"
     gene            complement(39716..41224)
                     /locus_tag="PK252_01555"
     CDS             complement(39716..41224)
                     /locus_tag="PK252_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13422.1"
                     /translation="MKGFVFLILYVLILIQALSGQGSTIPVNSVLYLYAENALFVDSD
                     FHTSVKPWVYSDERFDTSVVQNSFITSTGKISIRPETETGFRYSGDFGKQMLFVEGAS
                     IRYKPSGKFSIKASIMAGGQYFEKGTNGFDTLKVLPYCGKWFSQSEKIYFFPAITGSI
                     NYKPGNHFLFQAGYDRNFVGNGYRSVLLSENSAPYPFVKINFQSWRVNYFIMWAYLND
                     ISDNGFTDKYGVFHYLDINLTRRLSIGFYESVIWWGSDANTVRGIDPAYLNPVIFFRP
                     IEYSIHSPDNANIGGNASLRLGKRSYLYGQLYLDDLMVKELLKNSGWWGNKYAMQFGF
                     RTSKAYGEHQVSLLTEINMARPYTYSHTSTTLNYGTLYGSLAHPLGANFAEGVGIFRY
                     SNHSWYMMTKIVLAKVGLDTSIVNLGQDIYKSYNLRNRKNDYNVKLWQGQKEILKWSE
                     LRFGKWLDKNKHFSLYSVMGIYSKGSFSSKDVFFNIGLSTAIFNNERDIVLP"
     gene            complement(41229..42308)
                     /locus_tag="PK252_01560"
     CDS             complement(41229..42308)
                     /locus_tag="PK252_01560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT13423.1"
                     /translation="MYFSNPLSLVLAFITAIVIVMTSIPTIVNVARIKNLNDEPGRRK
                     AHKKIVPTLGGIAIYAGIIISGLLFIRFSIVPEFQYIVIASVILFFIGIKDDILIIAP
                     TKKLMGQIIASLIIIIMGDFRFTSLHGFLGYQIIPYWFSVILTLFVFIVIINGVNLID
                     GIDGLASGVGVLASVAFGTWFFLSGEYEYAIVAIAVFGSLISFFYFNVFGRNNKIFMG
                     DTGSLMIGLFMAVFAVKFCEVNIDVNTPFYIHAAPSVAFGILIVPLFDTMRVFIIRIL
                     KGHSPFRADRNHVHHKLLFIGLTHLKSTLLILGANLIIIITVFLLNDWGMIDLLLLIV
                     IMALVFSVMPETIFFIRQRRLKNIH"
     gene            complement(42387..43829)
                     /locus_tag="PK252_01565"
     CDS             complement(42387..43829)
                     /locus_tag="PK252_01565"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT13424.1"
                     /translation="MQKISRRDALKLLGSLSLPFIFHRELSAYFSKAEIKEMSAADFG
                     RFTWGVACASYQCEGAWDADGKGISVWDTFANDTSNIKDHTNANTSCDFYHHYKKDIA
                     LLKSMNFGALRFSISWPRIFPKGTGEINVKGIDFYNRVIDACIENGIEPWVTLYHWDL
                     PQALQNKGGWRNRDTVYAFCDYVKVCADAFGDRVKNWIVLNEPLSFSLFGYGIGIHAP
                     GEIGLNNFFHAAHHAALAQSLGGKVLRECLPNANIGTAISCSPIEPQDPNSKSDIRAA
                     RRLDAIMNRMFVEPLLGKGYPYSDASILHRIDKYMQADDEKHLSFDYDFWGLQYYFRV
                     VVNGNRAIPILQSTRVSPQKLGNEITEMNWEVHPEGLYKILKQFSQYPVKSLLVSETG
                     AAFPDKVQGTAINDAQRVKFYKEHLYWLLKAKYEGVKVDGMFAWSFTDNFEWAEGYRP
                     RFGLVYVDYDTQSRIIKDSGKWFTSFLENK"
     gene            complement(43838..45184)
                     /locus_tag="PK252_01570"
     CDS             complement(43838..45184)
                     /locus_tag="PK252_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT13425.1"
                     /translation="MARRFVISNILGLQVFQVLRFVSFLIISVIFTRIDLTKKDIGDF
                     ELLLFLASTVTFFWITGIIQSLLPLFNNNQSFKLRNGKVFQKSPELFNTFLLILFISL
                     LLFAAGWLFKDGIKVYKTGQALPYADGLLWYILLSSPACLIEYIYLLYNKPKHILAYG
                     LISFTLQTLFVIIPVKLGWGIEGGIWGLVIISGIRFLWLLRLIQKYAIIKVSLSFMKE
                     HLYLALPLIISSLLSGSAQYIDGLIVSAQFSPEKFAIFRYGAKEMPLVVTLASGLNNA
                     MLTQFSSMSKMKFGLLTLKKRSLRLMHILFPISILLLFFSDLIYPILFTKNFYQSSDV
                     FMVYVLLITSRLVFPQTILIGLKKNKVVMWTAVFNIIINVGLTFLMIPRYGIVGVAVA
                     TVIVYVIEKIILMSYNYFKLGINPNEYIPVGWYLFYSALITVIFVLIDHRIIMLRL"
     gene            complement(45255..46022)
                     /locus_tag="PK252_01575"
     CDS             complement(45255..46022)
                     /locus_tag="PK252_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13426.1"
                     /translation="MTITLGVITFSGCTKYQYITLSGNVNQNESKAYVFENDTVKVVY
                     SFNGNCFPMSFEIYNKSNFPLYIDWSKSSAIVNGHNISLWKDIATINASSSSEEYKFS
                     KNFTYSNSDITGTMAKSEQVTFVAPKSFIQRTPVYLRSEFIKQFDKSNKVRKNVYSKV
                     GKTSEVFYYFKPDSTPMTLRCFLTFSLKDNKSEPFYIQNEFWVSSISNTMLDPENLMY
                     KPSNQVYLQDLTGFGAVAWTVTILGVIGITCIAKAQQ"
     gene            46235..46858
                     /locus_tag="PK252_01580"
     CDS             46235..46858
                     /locus_tag="PK252_01580"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HOT13427.1"
                     /translation="MDAIKDQKTPPITLPQAVLAPLLSLFQAQVHAGRAFLSYLLQIG
                     SPHVEVDESGMIIEQARKEKEALYKQTFTFEKKVNDQTQKFELSVPTLSLVPIAPLGI
                     DSAEFEFDFRVENFQAHAQMQKSEKKALEKDAFSSDNRPWYLVENPINFSGNVVPPSE
                     EATATSKTQNVIKIKITVGKQPIPSGLDKFLVSLNQLAEVNEMKSVQ"
     gene            47396..50587
                     /locus_tag="PK252_01585"
     CDS             47396..50587
                     /locus_tag="PK252_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13428.1"
                     /translation="MKQNDIELKSNVSFKRAFLFLASLLIFTWAIGQEKTITGQIVDK
                     TGSTLPGVNIVVKGTTTGTVSDIDGKFSIKVPNENAVLQFSFIGYETQEVVVGSQTSI
                     PVTMNEAVSELNEVVVIGYGTQKKSDLTGSVASVSGAKLAEVPVANIAQALQGRAAGV
                     SITTNTGMPGGSVNIQIRGMTSINGTNPLVVIDGIPGGDMTKLNAADIASVEILKDAA
                     SAAIYGSSGGNGVILVTTKKGSAGKITTNVNYSYGWQSIANEVEMMGLKDYNRVLGEL
                     KLAQFTTNIDTLEYYNWNDLFFKKNVPMQSADFSVAGGSEKSNFYISGSYLEQEGIIK
                     KSNYTKTNLRVKADQKLTNHIKFDQNVSYSNTINEGYEEWMYTGVYQTPVYNAIIMPP
                     YMKGYQDDGKWTVTPGGVRNAMVMVDNRNYWSKNAVFEANAGLTIELFKGLTYTPRIA
                     GTMSILDNQNYTPKYFAKADDYQTFNLLEKNMQRGFNWMLQNVVTYNTSISEVHNVSV
                     MAGMEASRNWGYNIQGSRQNIASDLPNMLYFVKSLDDTSNFQNVTGTGYERKFAAYFG
                     RLSYDYKGLVLLQFNLRRDGQSDFGPNNRWGNFYSGSAGFKFSELEAVKNLGLISFGK
                     VRLSYGEMGQYPRSNWPYLAKILTTVPTYNYSFNNRVSQTGAGPVQIANPDIQWEKVQ
                     SQGVGLDLGFFKDKVMFSIDYFNKYNNNMLMYKDVGLVNGVFQTNSGGEGGPTRPEVN
                     SGTVKNSGIELSLSYKETFGELKSQLDFNLTYVTNEITDLATDSIVKGNVHNEFSGIT
                     ISKIGHSIGEFWGYQSDGIFRKGDPTLTDAKGKPFFYTINAKGDTVKYSTAVTAGDAK
                     WVDLNNDGIIDNNDRTYLGNPNPPFVFSFSINLAYKALDFSAFFNGVYGNKIFNGGKR
                     YFYDWNNRITNRAAAFADRYREPLTDKQGNLILDNHGVPIDEGNTSSSMPKMGVNSWG
                     RSSDLLIEDGSYLRLKSIQLGYTVPPKLTQKVKIEKFRVYVSVSNVFTLTKYSGYDPE
                     VARYDFSDPSILGVDIGGYPKTRMFTVGANLSF"
     gene            50607..52268
                     /locus_tag="PK252_01590"
     CDS             50607..52268
                     /locus_tag="PK252_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT13429.1"
                     /translation="MKTKIYKYLLIGLLFSFVMSCKEDFLNVEPIATDNEASFYLTQK
                     DAEMATTAVYAQLAYMTAWDRETSMLLGNMASDDADAGGEDPGDVTSFQDIAKLKHIP
                     SNENLRLPYGVFYKAIMLANKALEKLPNIPKIDKEASPAAINQMIAEIKALRALNTFQ
                     LTQIYGEVPLVDHVLGSSEFIQPRAKMKDLYAFMEKDLKEAIEVLPLSYDAENVGRIT
                     KGAAMAILAKIYLFGSSYAKNYPGDARFEGLTERWADALAMAENVMNLGVYKLLGADG
                     ETYSCWRGDKVSGFRYLFTTAGDNSAEAILEVQNIQDGLKWLSSRGSSIVHWSGPRYY
                     YDAKGVKQTTSYWGFDIPTKSLKDEFEAGDPRLDITIHMPGGNDSINLTGGWYKICFD
                     NSLTRMYQNKYECSYDEYRKNTGSWNEAPMNFKILRYGEVVLTAAEAAVMLGNNDKAL
                     KYINMIRSRARACGGPGNTVPADLTGTVTLDQVKHERRCELALEGKRFYDLVRWGDAS
                     AKLNGFVKGNDESVVFVKGKHEFFPIPQAEVDVSKGVLKQYPGWE"
     gene            complement(52617..54308)
                     /locus_tag="PK252_01595"
     CDS             complement(52617..54308)
                     /locus_tag="PK252_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13430.1"
                     /translation="MKIFQNRVLLNVLAIGLFLAFIFFIIKKNQDKKIIIKYPFNQAV
                     FPPEFPSPTFEWKYRGSYTGKWSFSVKSDSKLLVDTIVSTTVLKLSEPVWENIKKESK
                     GSEVQLIIKPLDKYGKASLVFDISKDSVGAPILYRQMPIPFVLAEENLDSMNYSLINI
                     GSKEPPHIAMKGFTVCGNCHSFSDKGKYIGLDLDAGLRDKGGYFVAPVNDTIYFNTNN
                     YMSWTQIENRKTFGLFSKLSPDGRYIVTTVKDRVIHKNFPFEPENLAFSQLFFAVNGL
                     LAVYDRQTGKICELNGANDEKYVHTNAIWTPDGKNIIFCRAKAVERIDSLEINITEEA
                     VIDEYVQRKRRLQYDICIIPFNEGKGGTALPIKGASNNGKSNYFPAISPDGKWIVFCQ
                     AENFMLLQPDSKLYIVPTAGGTAKELSCNLPLMNSWHAWSPNGHWIAFVSKGMSVYTD
                     LFLTHIDENGNSSAPVLVEKARKFRQVVNYPEFININAEKTFVMHYDYVEIAHIQRAV
                     LDHDYEKAKKLFYQFKRQPQRLFIEDYNQLERLMKKIKLDQEIAWIDTMRERCKQ"
     gene            54439..57189
                     /locus_tag="PK252_01600"
     CDS             54439..57189
                     /locus_tag="PK252_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13431.1"
                     /translation="MCKRLLWVILCCMVISSCNNSKIKNQCKDFLIDKSKISGISVVY
                     PQENTLFPIDFQSPTFVWKDSFTNNSKWYACISDSAGNILLGEFVENNKWKPDSTQWE
                     ELKKKNRDNSLKFAVIGFNENNENVTGNKLKFKISPDSVGAEIFFRAVTLPFSYAVKN
                     VNTIEWYMGNVSGGSPRKMLDNMPVCANCHTFSKNGPTLAMDVDYGNDKGSYTIEKAV
                     DTCFLNPENIISWTNFKKEENDPTFGLLSQISPSGEYVLSTVKDLSIFVAIDDNLAYS
                     QLFFPVKGIIGIYNRTTKQFSELQGANDPKLVQSNPVWSPDNKKVLFSRTNSYINERI
                     RKSGRALLYAKDVKEFTQGHKPFKFDIYSVDFNNGKGGQPSPLEGASNNGKSNYFPKF
                     SPDGKWVVFCQAENFMLLQPDSKLYIMEAKGGKPRLMNCNMRNMNSWHSWSPNGRWLV
                     FSSKEQSLYTRLYLTHIDEEGNDSPAVLIENLVFDKRAANIPEFYPGDAKKFVKIKDN
                     FSNTAGYYTQLAMDNIGSEYYLRASQNLERAINLDAEYLDAYIQRILLNALLKQANSK
                     TDLADKKKATDIIQKLIEKNGEDKALLILKAMIMSANGNNKQAEEILKKLITKEPNYY
                     KAYELLANIYKKESKTELIIPLYNKMSSLVPANDLQVKLSLAKAYISTNQNSQALALL
                     NTLKNKLPDNEEIRSLLCKIYVNTKNLSAATKEIDFLLAADPADYQYLFMRAEISQLQ
                     GSVYEANTYQEKGKMQLLKKLEANKENIPLAFEQAALLSQEKKYTSAIDVYNSILNVL
                     PSNYQAMKEKARIMLITQQWQEAVKLYQQLLKIYVPEEEFYNNKAIAQINMGNLQEAL
                     SEFNNVLNLSPTNIDALYNRAKLFKMLGEPHKAAKDIDKIKELLKAKKNLSESEKKLL
                     NI"
     gene            57739..58050
                     /locus_tag="PK252_01605"
     CDS             57739..58050
                     /locus_tag="PK252_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13432.1"
                     /translation="MKILFIVVALIVMPAISTYSQIKVLSSGAVNIGKTGYEMIVFDY
                     SGYNGTGPVLYPSGSNSAIQLGKAGKGFLCLYTTTVYYPSDARLKENITNITSPLQTL
                     K"
     gene            58066..58185
                     /locus_tag="PK252_01610"
                     /pseudo
     CDS             58066..58185
                     /locus_tag="PK252_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            58342..58668
                     /locus_tag="PK252_01615"
     CDS             58342..58668
                     /locus_tag="PK252_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13433.1"
                     /translation="MKEAVETTEETTTEVNTTQTNSIATLGQNAPNPFNQRTQINYYL
                     PETVKQAALYIFDMNGLQIRTIAINTKGNGSITLHASELNAGMYLYTLIADGKEVDTK
                     RMILTK"
     gene            58765..59445
                     /locus_tag="PK252_01620"
     CDS             58765..59445
                     /locus_tag="PK252_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13434.1"
                     /translation="MNNKYHLVRVIYLLFILQLIIVSVNAQKNIEHEIIKVSEANFKK
                     YLEKIPYGHEDYYGFQNRNEFEQVVVGEPYQILYLTNIEMPEDSINYDNLITYSDSWE
                     VPLLVNKEKRCFLHVQKIIKEYKVVGIGYNMVANELNETETRAIFNNKKVNKSILYLP
                     SIKGVYLLRDSLGNKNSRDLKFIPLGSTLKHVHDIKKETYSGVLIEYSMRDFMHVIKV
                     KYNQILNS"
     gene            59448..60227
                     /locus_tag="PK252_01625"
     CDS             59448..60227
                     /locus_tag="PK252_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HOT13435.1"
                     /translation="MKTKYYKYLVVLFLTVSQNIKCQVLNLPLLPEIQCNTWCWAISC
                     RHIIYYYGNDNITLCEIAEYARTRNPNRFGQTNCCQSIDNGCCNGNFLSGAGGMTEIF
                     SHWGLSPNYTFSPLTKNEITIEISNNRPFVIRYPGHVDICYGIVANDIYIRDPGWGFD
                     IRDYNTLISGTNNNNKWTETIRINESPVVCDFEKSLTGTISYSNIYKASGIYDVSCII
                     NNNSIITIKAPDSITLSPGFSIELGSSLELQTGSNLNINCP"
     gene            60242..61099
                     /locus_tag="PK252_01630"
     CDS             60242..61099
                     /locus_tag="PK252_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13436.1"
                     /translation="MKKIIQIITVLIVFVLNVHSQQLVEKDKTWSIAQFDDKNGLDTC
                     YYLKIGDDTLINDRTYSKVLISGDSLMTNWLEYDKYLCQDQNVVYCYSKSVQNEYVLY
                     NFNLSIGDSIKVEGNGFYIYLDSIKTKNTKKYFYFSRNGEITTWVEGVGCLDDLFQNS
                     GNINIVGGYSTLLCCKIGNDVLYHNVKYNNCFLTTSIKRSVDEAIVFQNYPNPFNDKT
                     YININNYYNTKAFLCIYNVSGVLCKKIEVRQYGANNICIENLAKGVYFYNFTIDGKYK
                     SQLKRMICY"
     gene            complement(61129..62259)
                     /locus_tag="PK252_01635"
     CDS             complement(61129..62259)
                     /locus_tag="PK252_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT13437.1"
                     /translation="MKFADVIGHSDIKKRLINAIRENRVSHAILFAGPEGNGGLQMAI
                     AFAQYIHCSNKTETDACGKCPSCLKFEKYIHPDHHFVFPTVNTEGSRKQSSNDFLPQW
                     REMLHEQGYFSLNNWTEKISNENKQTGIFEHESSEILRKINLKPFESDLKTMIIWLPE
                     KMNETVSNKILKILEEPPLKTYFILVSENPGMLLKTILSRCQLTHVPPIDAESLVAGI
                     RAKFPDVENPEIIARMAAGNFVKAGEFIRENENERDNFAYFKSLIELVQKNDVINLVG
                     WSAQMATLGREKQKSFFNLSLNLIRNSFVYNLTQNTALLYQNTEQQEFSKRMNAFFNQ
                     ANCDRLAQLFNAGASNIESNGNANIIFLDTALKLRPLLKAGA"
     gene            complement(62281..62712)
                     /locus_tag="PK252_01640"
     CDS             complement(62281..62712)
                     /locus_tag="PK252_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13438.1"
                     /translation="MSKAKTFIKYTLIIGLALAVIGFIVLLFVFRKADDTVAKQKTDY
                     SVQSKQLVTDFENNENTANAKYLGKILEIEGPVAEITVDSNAVVVVLRPEGAMSGVNC
                     ALGKSQVADTSKISIGKTVKIKGVCNGYLMDVVVNKASLVK"
     gene            complement(62772..63323)
                     /locus_tag="PK252_01645"
     CDS             complement(62772..63323)
                     /locus_tag="PK252_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT13439.1"
                     /translation="MKHILFLVLSLCFICTTYSQDKYFTKTGKAYFMSHTDVIDIDGT
                     NNQVFCVLETQTGNVACAMLIKAFEFTLATASEHFNETYMESHLYPKANFKGKIAGFD
                     MAKLSSAETKLTAEGQLTIHGKTKNVSLPVVVSLKDGVIVGSSNFKVSIADYDIKVPK
                     VVENRVAKIVDVKINLELKAEKK"
     gene            complement(63339..64241)
                     /locus_tag="PK252_01650"
     CDS             complement(63339..64241)
                     /locus_tag="PK252_01650"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOT13440.1"
                     /translation="MKYSYIIFLATFISTIGLKAQDDLDAFLDEQMADHVDYALGTFY
                     SSRIVNFQSTQMIPKGGLDFRVHHRFDQVSEGYNRFWGIDGSHSYLSLEYGITDYLMA
                     GIGRQSDAFFNGFAKVSVFRQQKSSKRTIPVNVVWVSTMAADAYNYENDKERNDDFSR
                     RCSYTHQLLISRMFSPKLSLQLSPTFVHRNMVETPQQDNDYYALGIGGRYKLTNTFSV
                     NAEYFYVIGDRNTATQKYYDPVNIGVDIQVSGHVFQIMLTNSSSMLEPGMLTQSSESF
                     TDGDIRLGFNISMVFTLGRKTTQQ"
     gene            complement(64262..65008)
                     /locus_tag="PK252_01655"
     CDS             complement(64262..65008)
                     /locus_tag="PK252_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13441.1"
                     /translation="MKHNYFTWAAITIIAMLAACTYQNEEDWKKEHASTDSVYQYGTK
                     GLIAFFAFDNSLNEKTNNGQTVEAENEVSYSNNTRGALSKACLYLNGNNSIKVLNQKY
                     DTLTVCFWLKSEQQLKYLSEPKPVLVDIGQGATVFEVDGTTGASDINLIVGDQKTEDK
                     TNTIDTYTEWNLVIIEIVNDGVNKLIFYNKINGEDAWRLLPKNIDYKNGQVCIGKSSA
                     NKGFFKGYIDDLLIYKRALTKDERENLKKF"
     gene            65211..65795
                     /locus_tag="PK252_01660"
     CDS             65211..65795
                     /locus_tag="PK252_01660"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT13442.1"
                     /translation="MKHELPVLPYAADALNAVISPKTIEFHYGKHHQAYVNNLNNLIP
                     GSAFENASLETIIREADGGIFNNGAQVWNHTFYFMTFSATPQAEPTGPLADAIVKKYG
                     SFADFKAAFAKSATTLFGSGWAWLVLRPDNTLDIVQEPNAGNPMRQGLKPLLTCDVWE
                     HAYYLDYQNRRPDYIEAFWKIVDWKIVSERFLKQ"
     gene            65923..66711
                     /locus_tag="PK252_01665"
     CDS             65923..66711
                     /locus_tag="PK252_01665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT13443.1"
                     /translation="MNYIEAFILAVVEGITEFLPVSSTGHMVASSTLMGIAHDDFTKL
                     FTIVIQFGAILSVIVLYWKKFFQSINFYLKLFVGFIPAAIFGVLFSDQIDAMLENAII
                     VGIMLIIGGVFFLFVDKLFEKTQVDDNLPGYKKSFIIGFFQCIAMIPGVSRSAATIIG
                     GLSQKMTRKAAAEFSFFLAVPTMFAATTKKLYDFYQDGIMMNAEQVKILAFGNIVAFI
                     VAIIAIKSFITFLTKHGFKLFGYYRIIAGILILVMVMFGFQLKF"
     gene            66713..67411
                     /gene="truB"
                     /locus_tag="PK252_01670"
     CDS             66713..67411
                     /gene="truB"
                     /locus_tag="PK252_01670"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT13444.1"
                     /translation="MTAEDFKAGQVILIDKPLTWTSFNVVSKIKYALKRQLQLKDLKV
                     GHAGTLDPLATGLLIVCTGRKTKEIESIQNTEKEYIATIEFGKTTPSYDLETQYNGQF
                     PFEHIDEEAINKAFQSFLGESLQVPPVFSAKMIDGKRAYKYARKGTEVEMRPNIINIS
                     ELEIISFERPVLVVRIKCSKGTYIRSFAYDLGKALQSGAHLKGLRRTASGNYHVNDAI
                     SLDTFQEKLVTLQP"
     gene            67471..68520
                     /gene="queA"
                     /locus_tag="PK252_01675"
     CDS             67471..68520
                     /gene="queA"
                     /locus_tag="PK252_01675"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT13445.1"
                     /translation="MKLSQYKFALPAELIASYPSPNRDDARLMVVDRKTGEIEHKIFH
                     EIINYFEENDVMVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGQDDSLVAEVIDNTTSRGRTLRFLFDGDYDAFKETLYSLGETPLPKTI
                     KRPVEAEDRERYQTVFAQHEGAVAAPTAGLHFSKPLLKRLEIKGVEFGFITLHVGLGS
                     FRSVDVEDLTKHKMDSERIIISEEVANIVNKAKEGKGKICAVGTTVLRTLESSVTTNG
                     TLKPYDGWTNKFIFPPYDFSVPNSMITNFHLPLSTLLMTVSAFAGYDLLFKAYKVAIK
                     EKYRFGTYGDAMLIL"
     gene            68536..69795
                     /locus_tag="PK252_01680"
     CDS             68536..69795
                     /locus_tag="PK252_01680"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOT13446.1"
                     /translation="MDKLLAIVFLCWGIYCLDAQTKNDTICDKDIHTIQLYVEGWEMS
                     NPVIFLNSQDKLHLSFDDLSGNPKKLYYTIIHCNEKWEQENVSYYEIAEGFESNPLMD
                     YKYSVSTVVNYLHYDLIFPNENCRPIVSGNYLLIVFADNDIEKVVFQRKFYVVEKSAI
                     IDFRIIKPEIARYMQRQHQFYLVVTPDVQNSIDLRSEIKAVVVQNFNFNTRKTCYLAQ
                     LQDNKILVYDNPDSNLFMGLNEFRNFDIKSIRYQSPRIKSISYLMNWYEVVLHPDSWR
                     NKKQYFSEQDINGKFFIENSNGKTKDIDADYVMVYFRLLTPDSLERGNLYVNGAFNNW
                     QCNEYSRLKYDTLEHAYKINLLLKQGYYNYLFVYKEAPSSIPDYVFVERSHYETENEY
                     QAFIYYQMPGERYQRLIGYAVANSLHK"
     gene            complement(69812..70453)
                     /gene="lpcA"
                     /locus_tag="PK252_01685"
     CDS             complement(69812..70453)
                     /gene="lpcA"
                     /locus_tag="PK252_01685"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOT13447.1"
                     /translation="MLQSIAKGIFVEIILTVEKLMTNDIEKDLLEALHTLQCFIEDTQ
                     QVKNIDNASQMLVDSIKAGGKIISCGNGGSMSDAMHFAEELSGRFRGERPALPAIAIS
                     DASHITCTGNDYGFDAIFSRFIESIGKPGDVLLAISTSGNSANVLKAAQVAKQMGIKV
                     LALTGKDGGKLASIADVEIRAPKTQYSDRTQEIHIKVIHILIRLVEEKLFSTK"
     gene            70918..72864
                     /locus_tag="PK252_01690"
     CDS             70918..72864
                     /locus_tag="PK252_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT13448.1"
                     /translation="MNFRRFFPRFQAEQYLVLLYRIFIVYLLYSLSRLIFYLYNIDHF
                     NNIGFDRLMIIFGGGLKFDTTAIIYTNLLFIVMSILPIPWRHNQGYQKVLKYIFFITN
                     GIGLVANFADIPYFNFVLERTTIKVFAQFQHETNMVLLFMRFIVDYWPITVLYIFSMF
                     LMVYLYNRLDVGKAFPKKWYFYYPYSIVVACIVILLSIAGIRGDLKHSTRPITMSNAG
                     QYVKSPAELAIVLNTPFCLIRSTEGVAYSRQNYFADEASLEKVYLPVHYPDTTRPKHP
                     VNVVIIIVESLNKEFIGSYYPEIDNGQYKGYAPFLDSLLKHARSYQYSFANGRKSIDA
                     LPSVLASIPAIESPFVLSPYYSNTMTTLPNMLKKMGYKSALFHGAPNGSMGFLAFGQL
                     AGVDEYYGMTEYNNDKDFDGTWGIYDEEFLQYMANTIDTFSKPFLATVFTVSSHHPFK
                     LPARYDKRFVSKDFPLQRCIEYTDYSIKRFMETASKMKWFNNTLFVITADHVSLNQRP
                     EFKNDIGYFAVPIIFYHPGSSLNGIDKKTIAQQIDILPTVANYIGYPEPYFSFGQDLF
                     NPPANKFAVNNLDDIFRFYQDGYLFMFNAKGPFHLYNMIEDPLLTKDLLGKEPKIQEK
                     LELTAKAFIQQYKNRMLDNKISLK"
     gene            72960..74786
                     /locus_tag="PK252_01695"
     CDS             72960..74786
                     /locus_tag="PK252_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT13449.1"
                     /translation="MRFGLILLGLQFCFNVGLFAQDKNTHFSILRNDIIRCMDKKLDS
                     AFFYAEQLEKDASKSKRPDYVSIALQYKGKINIRREEFSTATGYFIDALRIEQARNNE
                     AQIAEIKFMLGYIFYLMEKYEKSKGYYQAALEFYLKNKDSLGMAKTYSHIGNLETSRE
                     YCENRSIPQKAEDYYLGINFYKKSLEIAHKIGNKEGEGIAYHNIGNTYRKLEKPGLAI
                     SYLLKAKQLYAEIHDTNRLMGLKRPLSMVYSMIGQHRQAICELTECIDYSVKNNQLQG
                     IQFLYEDLAYIYERAGDYKRSKENYVIYMTIRDSIYNSEKTHKIFELETRYNTVQQEK
                     QIMKLTAQKKLQRAYLGISVVIILLLLGLGYFIYKALKNKRVLAERNLEIHEQRLVQL
                     EKDHKILASQLIIKGEETERQRLARDLHDGLGGMLSGVKLSLSSMKGNAIVSEQSAKE
                     FDHALNMLDASIKELRRVAHNMMPEALIRSGLKDALSDFCASLNESCQMNISFQFVGA
                     FQRIKPELEINSFRIVQELVNNALKHSQATELVVQMIQEQDRLCLIVQDNGIGFGPEI
                     AKLQKGMGIDSVKSRVELMNGSIDVASKPRQGVEYTIEFSLL"
     gene            74897..75505
                     /locus_tag="PK252_01700"
     CDS             74897..75505
                     /locus_tag="PK252_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT13450.1"
                     /translation="MSQILIVDDHPVVVEGLQKLVTDKKIARECIVAFSCAECLKIID
                     IYTPDLILLDYQLPDGNGINLCSQILKKMPEAKILAISSFKELSLVKQMIAGGALGYV
                     LKNASDVEIEEAIRKVLAGKKYLSDDIAESMQHADSNFILTRRELEILHFIADGFTNP
                     EIAEKLFISPLTVDSHRKNLIVKLNAKNTAMLIKVAAQKGFL"
     gene            75529..76047
                     /locus_tag="PK252_01705"
     CDS             75529..76047
                     /locus_tag="PK252_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13451.1"
                     /translation="MKKFIIKNIYKAFILLGIIIAACNKQPVNIVNQKVNIRYDSLNF
                     ISLADTIVCDMVIKNPDKDDKWTEECLQQLQRKKLIDTLFYQVYNNELQVLDYYTNTT
                     LCPSEIRKIEKEFSNNRDLIGKIQFRETWMYDVKNKVFIKKVHSMILGYETFDDKGFV
                     KGYKPLFKIMLP"
     gene            complement(76481..77017)
                     /locus_tag="PK252_01710"
     CDS             complement(76481..77017)
                     /locus_tag="PK252_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13452.1"
                     /translation="MKMSSGMFWGLLLIVIGISLIIKIVFNIDFPIFRLIVAFIFIYI
                     GIKIIVGKSFHPFSRHKTDTEVVFGESSFDNIEPGKEYSVVFSKGVIDLRGVQAKREG
                     GKVKISTVFGSSVVIVDKSFPVKINVDAVFSGAHLPNGNSTSFGTTIYESDSLDVNNP
                     YLEIKADVVFGDLKIEER"
     gene            77251..77841
                     /gene="grpE"
                     /locus_tag="PK252_01715"
     CDS             77251..77841
                     /gene="grpE"
                     /locus_tag="PK252_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT13453.1"
                     /translation="MKKAKHEEVKMENDAQQPTGTDNISAQQNEQMTEQNSNNAEDVE
                     KTEASVAQFEKEAAEWKDKYMRLSAEFDNYRKRTLKEKMDLTKYAGEEMLKSLLPVVD
                     DFERGLATLEQSNDIDAVKQGIELIYAKFISFIQQKGLKEIEAKNLPFDTDFHEAVTK
                     IPVEDKEMSGKVVDVIQKGYLLDEKVVRFAKVVVGE"
     gene            77869..79020
                     /gene="dnaJ"
                     /locus_tag="PK252_01720"
     CDS             77869..79020
                     /gene="dnaJ"
                     /locus_tag="PK252_01720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT13454.1"
                     /translation="MSKKDYYEVLEVPKNATKDEIKKAYRQHALKYHPDRNPGDKAAE
                     EKFKEAAEAYEVLSDDEKRARYDRYGHAGVGGASGAGGFGGGGMSMEDIFSHFGDIFG
                     GHFGGFGFGSSSYQGQRVSKGQNLRVKVKLTLEEIATGVEKKIKVNKYVQCSSCNGSG
                     AAKGSSHTTCSTCHGQGVVTKITNTFLGQMQSTSPCPSCGGEGKIITQKCTRCYGEGI
                     VKEDEIITIKIPAGVAEGMQMTVQGKGNAARRGGINGDMIVTFAEEKHPELLRDGNDL
                     YFSLFVTFPVAALGGTVEVPTLEGKVKIKIEPGTQPGKLLRLKGKGLPEVNGYGKGDL
                     LVHINVWIPKNLTKDERRIIEKFAESSNFDPKPEKDEKNFFEKIRNLFE"
     gene            complement(79122..79192)
                     /locus_tag="PK252_01725"
     tRNA            complement(79122..79192)
                     /locus_tag="PK252_01725"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79158..79160),aa:Cys,seq:gca)
     gene            complement(79267..81306)
                     /locus_tag="PK252_01730"
     CDS             complement(79267..81306)
                     /locus_tag="PK252_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOT13455.1"
                     /translation="MSENKFVPFVPANTNMAEMTIRALLIGLVLSVVLGAANAYLGLK
                     AGMTIAATYPAAVISLAILKAFRGTILEENFARTVGSIGESVAAGAIFTLPAFYIAGL
                     WDPFYSPGNYITASLILIAGGILGIMFVALLRRIMVDDVELPFPESVAAAEIHKAGRS
                     SAGNSKFLFIAIGVGGLVKLLGELRFFAVYWDKFIVFAKQTITGTSIKAKGGILAGSP
                     EISPAYLGVGYIIGPRLAALNFSGGILAWGILIPMILYFLLPGIDMESLKTAMIAANP
                     GISPDQAEQSITIGLIAQIARYIVKPIAIGGMLVGAFYTLFKMRKSLIAGVSRSVGDV
                     KKAMAGHELNIPRNEKDLSFGWILLGIFGVAILTFIITFFIFHTTLLVAFVAATVLLI
                     LAFFFAAVSGYLVGIMGSSNNPISGLTLTALVVTALIMVLMGVRGQEGVAAVLGVAAI
                     VCVSAAVAGEMLQDLKAGHILGGTPWKMQTGDIVGVILSSLVMFGVLVLLNNGDIAQG
                     VRDGYQGGFGSSNLSAPQAGLMALLSKGIVGGDMAWPLIIAGMLLGASLILMQAKSPM
                     LICVGIYLPLQTTFAIFIGGMLKGIADMIADRNKYSPERKAIAENVGILIASGLIAGE
                     ALMGLFLAGFRGFDIFLTDYFYFFKNPSFMISLIVLAAVSMFIIWWPLRYSKTDN"
BASE COUNT        25682 a        15269 c        14950 g        25424 t
ORIGIN      
        1 tcccagtttt gttggcccat caataccggg acacacaaga ggcaaatagc agtgatgcct
       61 ccggctttca aaaccgatgg tattttgtcg gatagcattt cgtataatgc agcaacccca
      121 atgccaatcc agatagcaaa tgcataaaat gaacctgtat aagcataatc acgttcgcga
      181 ggctggtatg gggtttggtt cagataaagg acaatggcta aacctgtaaa gaagaacaat
      241 aacataacaa ctgagaagtc ttttttgtct ttcttgagct gataccaaag ccctataata
      301 cctaaaataa gaggtaagaa aaaataagta ttgcggcctt tattgttttt catagaatcg
      361 gggagcttat cttgagggcc taaaaagatt tcatctataa acttaatgcc gcttatccag
      421 ttacctttag tgggttcacc tgcaccttgt atatcatttt ggcgtcctac aaagttccac
      481 atgaaataac ggatatacat gaaatttacc tggtaacgga agaaaaacaa caaattttct
      541 gagaatgtag gtttcataat ggttgtaggt tcaccttttt ggttttgcgt ctgaataggt
      601 acacctttta ctttgcccca gtattcatag gcgctttgat gcgattgttc agagctgtac
      661 atacgaggga aaattgttaa aaacctctcg tcatatttgt attcaggttt atggttggta
      721 atttcatacc ttccgttgat aggggtgtaa gtaggtttgc cattttcttc catccccaaa
      781 ataggggtat tgtaatatgg gcccgagaat aaaggcctgt cgccatactg ctcgcggttg
      841 aggtaagaaa gcagcgaaaa catattttca gggtcgtttt cgtccagcgg ggtattggcc
      901 gaggagcgga taacaaccat tgctaccgaa gagtagccaa taaggataac agaaagcatt
      961 agcaatgcag tattaagtac cactttgcgt tttttatagg tgtaccacaa gccccaagct
     1021 aataaaacaa ccaaggctac caggtaaaag aatactccag agaagtaagg tagcccaaag
     1081 ccattaacaa acaaaagctc aaattttgaa gccaaaacaa tcaaacctgt aatcacacca
     1141 tacatcacac ctgccaggat ggcaatagaa actaatgaag taataattat acctttagtt
     1201 gtaacagggt attttttgaa ataataaaca aaaacaatag caggtattgc caagaggttc
     1261 aaaaggtgta cccctataga aagccccatc agataggcta tgaggattaa ccacctgttg
     1321 gcatgtttat catcggcctg gtcttcccat ttcaatactg cccaaaatac aagggctgta
     1381 aacaatgacg atgaagcata tacttctgcc tcaaccgcag agaaccaaaa tgaatcagaa
     1441 aaagtaaaag ccaatgctcc tactaagcct gaccccaaaa cagcgattgt tttgcctgtg
     1501 gtataatttc cgtctttgat taataatttg cgacccaggt gagtaatagt ccaaaaaaga
     1561 aacaagatgg tgagcccact tgccaatgca gaccaactat tcatccatat tgctacttta
     1621 gttacatcgc ctccggcaaa taaagtgaag aaccgcccga taatcataaa caaataggct
     1681 cccggagggt gcccaacttc aagtttaaat gcagtagaga taaattcgcc gcaatcccag
     1741 aaactggctg ttggttccat agtcaaaaga tacaccgctg tagctatagc aaatgtaacc
     1801 catgcaataa tgctgttaaa taatttaaat tgcttcatta tttgtccttg ttagattttg
     1861 tgcatacttg tttgcaaata aaaatttttg taaaaacaaa attttgccca aagtaagaca
     1921 atttttgcaa aacacattta atgacctgca aaattgttgc ttttttaacc ttgtttaaga
     1981 atagcccaaa ggaaaaaggg gaaagacata aaaaaagttg tattataagg cctgccgcgt
     2041 aagcagtaga gctatgtttt attattgctg gtttgataaa gtaaaacttt gaataataat
     2101 ataattagca gcaacatagt tgcgatttat ttgtaaatat atgatgttga tgtagaaagg
     2161 tgcgtagcac cggtacatat cgccactacc tggctttgta tatactgtcc atttttacaa
     2221 atatctcgcc gccacgcggc taagaatagt atttactacc tccatgcttt atttattgcc
     2281 agggcaattt cttcaaattc ttcttttgtg agttttagct tgggattggc aaagttcata
     2341 tcttgcatgc ctgtaataat gggtattaaa tgtatatggg catgtggcac ttcaagacca
     2401 ataactgtta gcccaacacg ttgacacttt actacttttt caatggcttt tgctgtacgt
     2461 tttgaaaata ccatcatttt ttcgagcaat gcatcgtcaa tatcaaacag gtagtccacc
     2521 tcttttttag gcactaccag cgtgtgccct tttatcaggg ggttaatgtc aaggaatgca
     2581 aaaaagttgt cgtcttcagc taccttgtag cttggtattt cacctgcaat gatgcgcgaa
     2641 aatatagttg ccataaattc tgtttataat ttattaatca aaaataaaaa gaattagaca
     2701 gtttctgaac agcaagttta ttaaattttc aaattattct tatctaattt gaggggctga
     2761 tatatgggta tttattggat attgtttttc cttgttttga gggttatgac cgtattttag
     2821 aaacactcat acaatactta ctaaggctct tatagcgttt agttttaata tctgaaagca
     2881 gttacaaact ttaaaaaagt tttagttgtg agcccgtata ggttgtccta attagaaagt
     2941 atgggagttt tcgttacaca ttaaacctaa agtgcataat gtcgccatct tgtaccacgt
     3001 attcttttcc ttcaatagat aattttccgg cttctttgca tgcagcttca gagcctaagg
     3061 taacatagtc attgtatttg attacctcag cacggataaa gcctttttca aagtcgctat
     3121 ggataacgcc ggcggcttgt ggggctttgg tcccgcggat ataagtccag gcccttgttt
     3181 cgtcagcccc ggtagtgaag aatgtttcga ggttaagaag tttatatgct gatttgataa
     3241 gccttgcaac gcctgattct tctaatccca gttcgtccag gaacatcttg cgttcttcgt
     3301 aggtgtcaaa ttcggcaatc tccgattctg ttttggcagc aacaattagt acctctgcat
     3361 tttcgttttt tacggcttct ttgaccatgt caacatattt gttgcctttt ataacagctt
     3421 tttcatctac attgcataca tacaatacag gtttatctgt aagcaaaaac aggtcattag
     3481 ccagtttctg gtcttctttg ttgtcaagtt taaccacgcg ggcactttgg ccatgcaaca
     3541 atacttcttt atattgaagg agtatttcca gtaatcgttt agcctgtttg tcccctccgg
     3601 tctgtgcttg tttttgcact ttagctatgc ggctttcaac agtttcaagg tctttaagct
     3661 gaagttccat atcaatgatt tccttatctc tgaccgggtt gatagatcca tcaacatggg
     3721 ttacattttc atcgtcaaag caacgtaaaa catgaatgat tgcatcagtt tcacgaatat
     3781 tggccaaaaa tttgttcccc aacccttcgc ctttgcttgc cccttttaca agacccgcaa
     3841 tgtcaacaat ttcaacagtt gtaggaacaa tccttgcggg tttgtctatc tcggccagtt
     3901 tagtcaaacg ctcatcaggg acagtaatca ccccaacgtt gggttcaatg gtgcaaaacg
     3961 gaaaatttgc agcttgggct tttgcattgg acaaacagtt aaaaagagtt gatttaccaa
     4021 cgtttggcaa acctactatg ccacattgta atgccatact aattataatt aagggtgcaa
     4081 agataatccg aaaaattact ttgtatttcg attaagcatt tttaatgctt aatttgggtt
     4141 taaagattaa aagagcattc taaaagagat agaactacaa atagatacaa aagacgactg
     4201 tgtgaaatta gaaaaatgct gattatttag aaattacttt atctctttga gaaacaccac
     4261 cctgtctttg tcgatgccag aataattctt tactacatta atgccgttgc tgtctggttg
     4321 gccaagtaag gtcattccta ttttgttttt atgaaaacta accgatttga agtttgttgg
     4381 ggtggtaatg gcttttagtt tctttatccc tttttgctta gcaatattaa taaagtgttc
     4441 atacagcatt tgtccataac cctgacgctg gcatgattct ctgactccca aaagatggac
     4501 ataggctaca ctttcggttt gggaaaagaa cccgaacaaa taggctataa cctggccttt
     4561 ttctttgata acaaaagctg tattgccaaa ttcatagact aaataagggt gatgaaggta
     4621 caatgttcgg tcactacccc aaaaatcgac aatatcccgg gttatttcgt tgaagtcgtc
     4681 catggtacaa ggcaatatga gggttttctt cttcatggct tataaattta tttaagttca
     4741 acagaaggat tccaaaaaag cttgtcaaag ttgacaatgt ggtcgttggt aatctctacg
     4801 ccttcattct caagcaattg ctgcattatt gaagggttcc caaaatgatg tttgccgcta
     4861 agcatcccat tgcggttaac aacgcgatgg gcagggataa actcttcgag gtgatgacag
     4921 ttgttcagcg cccagcctac cattcgggac gattgcggag agcctatgaa acgggctatt
     4981 gctccgtatg tcgtaactct tccatgaggc acaagctttg taacattata tactttctga
     5041 aagaagctgt cggtcatacg tttggtcgat atcaataatc tcttgctgta gcaattggaa
     5101 ttgaaggtaa gttatttttt ttgttttttg tatgaaaagt ttttcgtagt gggtagttac
     5161 gccataagtc aactggctgt atgggctgtt atacaggtca tcggtagcta ctttaagcgg
     5221 caactggtta tgctcaataa ccgctttggt gtatttgtgc agaaaaaggc tgtcagtttt
     5281 taggtgtatt atgccttgcg gctttaagaa atttttgtag cggttgagaa acgaaggggc
     5341 tgtaagcctt ttgcgcctgc ggttgctttg catttgaggg tctggaaagg taacccaaat
     5401 ttcgtccact tcgtctttac tgaaaaacga ttcgataaat tcgatgcggg tacggacaaa
     5461 gtaaacattt gtaagccctt tttcaagggc ttgtttggcg ccaacccaca ttcggttgcc
     5521 tttaatgtct atcccgatga agtttttgtc aggatatatt tgtgccaggg caactacata
     5581 ttcgcctttg ccgcatccca attcaaggat aataggatta ttgtttctga aaatatcttt
     5641 gtgccaggtt ccttttaccg cgtggtcttt ttggtacacc tggttataat cagcttcaat
     5701 cacgtgctga aatgtatgca attcggcaaa cctcctgagt ttgtccttcc ccataaatag
     5761 taattgttat tgctttgatt tttcaatgat gatgccaaca tcaataattc cttccagctc
     5821 attctcgcgc atgccgtgag ttacataaaa catgtatttc ccgttttgcg aaaaacggac
     5881 agccttcttg taccccaatt taatgttttt gatatccgaa aggcctttgc caatccagcg
     5941 cccttttgca tctgcgaggt tatattccag ggtgtctata attttattac cattgggggc
     6001 catggtagta acaaaaaggt agatattgct atacttgtag ttgcctgagt gcctcaggtt
     6061 gaaataaacg ttactgggtg aaacggtgtc attattgatg tattcaaacc ggcatttgtt
     6121 cgaaattttc cacttgctgt ctgcaataga aaaattttga acatattgct ctctgtttac
     6181 acagcttaac actgttaaaa caactaaaac taaaaggcta agctttgttt tcattgggct
     6241 tttgctgatt gttctgtttg ttttgtctat tagggttgtt ctgaaatttt ggtcggtttg
     6301 ccccccccgg attatttggc ctattagcat tttctgggtt gttctgcctg ttgttggcgc
     6361 tgtttggttt gttaggccta tttttttgaa actgttgctt gtctttcttt ttcttatcgt
     6421 caaaacgggt aaggctgtct tgtccgacca cattggcgta atcaagctcc tcgataaccg
     6481 gaacagcgcc ctgaggttta agttcttcac tatctaactt tactttaata ccttttttat
     6541 tagattcaat ggtttcaaaa actttttcga cagaaagggc aataaaattg gtttgttcgt
     6601 ttttgtttag tgcataccac atcactcgtc tgaatacatc agttttttgg tgatatgcag
     6661 ggccttcggc ggtttccaaa acaatgttgg tgtccgggaa gtctttttgg gcatcgaggt
     6721 aagcatcgag ctcatagttg aggcagcact tgagcttacc acactgcccg gccagttttt
     6781 gggggttgag cgaaaggtct tggtagcggg ctgcattagt tgatacagat acgaagttgt
     6841 ttatccatgt cgaacagcaa agttcgcgcc cacaagggcc aatacctcca atacgtccgg
     6901 cttcctggcg tgccccaatc tgcttcatct ctatacgtac acgaaattct tcggccaaaa
     6961 ccttgatcag ttcccgaaag tcaacacgtt cgtcggctat atagtaaaag atggcctttg
     7021 acctgtcacc ctggtactca acatctccaa ttttcatgtt aagatttaga gagtcgctta
     7081 ttttacgggc ttttaacatg gtactttctt ctaagctaat agattcgtac catttgtcaa
     7141 tgtctgtttg ttttgcctta cgataaagct tgcgataatc tccattttct gtacgtacct
     7201 tataacgctt gatttgttgg cgtacctgct cgccaacaag cgacacaatg cctatatcgt
     7261 ggcctggcga tgcttcaacg gcaacaatgt caccttgttt aatttctaag ccattgacat
     7321 tgcggtaaaa acctttgcgg gtgtttttaa aacgcacttc tacaacatcg taaggaataa
     7381 ggccttgagg taagtcgtat gcccagttgt atgtatttag ttttgaacat ggcgaagggt
     7441 tgcatttgaa ggtttccatg ttatcattta caacttcaga acaaccccgt aatatgatat
     7501 ctttttgcat aaaattctat ttagtgttac gaagcaagaa ccaagagcta agaaaaaagc
     7561 tgattggtaa gattttactc catgaacaaa gtaataaccg atttgattaa aaaatctgac
     7621 aaatctaatt attattttaa taaatgaggg agcatatata agatttatac atactccctc
     7681 attagtaaat tagcaataaa tgttgaagaa aattattctg gcggctattt tacaataact
     7741 ttcgagcaaa attgagcccc tccgtttgac attcggacaa aatagaggcc ttgtcttgaa
     7801 ttggggactt taatctcatc tgtagccagg ctgccagatt tataaattga ttttgctatc
     7861 actacttttc cggtaatatc caatacttct gctttccaag taccagtatt actgtttact
     7921 aaagaaatat tgatgctttt tgatgcaggt tcccatgaac atcttaaatt atcgccactt
     7981 gtgattgagc ttttaacatc agtttggtta gaatcattca ataatatcac gcttccaaaa
     8041 tatgatgcgt ttatccaact aacgttttta tcagcacctg caatagctac agaaccaaac
     8101 atactttcac gcgttgagcc attgtcatta tcgttgtatg caactgacat tcccattatt
     8161 ttgttgagtc caagagttac aggtgtatta ttccctccgt aaacataagt attgtcaaaa
     8221 acttttatac ctacttccca taaatagaca ttttcagaaa tggtgtcata tacactaact
     8281 acatcatttt taaataagtg attaaccctg tcagtgccta aatcgcatac atcaccattg
     8341 gccgcaatat gataggcaaa tgcattgaag ttgtattgat ggtcaccgcc tgagcggtct
     8401 tcgtccagaa atatttcaat acaatcaaag ttatgaatat tgttacagtt gcccggatac
     8461 tttgggtagt gcttgtcatc agtaatttct accaggaagt atagttgttc aggagtccat
     8521 acaactttat atttaccgct aaagtcggca tcattggttt ttgagccaat ccacgtttgg
     8581 tcaattttag cccaaggggc tttttcccaa caggcatcag agccattggc atcaacaact
     8641 ggcgcaacat ctgcttttac agctttataa ctttgtgcat ttatagaaaa tatgcttaat
     8701 ataaaaagag cggagataag aattgttttt ttcattatag ttttattagt tgcccaaaaa
     8761 taaagaatag gattaatcgg ggcaaagtta gttattgttt taacattaat ttcagaaatt
     8821 aatattaaag cactttaaag cactaaaatt caaaggtgcg ttaaacattg ttaaattgag
     8881 ttaatggatt ttaaaagcat ttggatataa aaaaatgcca tttttatttt tgtcttactt
     8941 taaacaatac gaaataaatt caaataatca ttcaattatg gatatcaaag tagagaagct
     9001 gactaagaat tacggccatc ataaagcggt ggacgatatt tcttttgagg ttaaaacagg
     9061 cgaaatactt gggtttttgg ggccaaacgg tgccggaaaa acaactacca tgaaaataat
     9121 aaccaactat ctcaattgcg agcaagggga tattattatt gggggcaaga gtatacgcga
     9181 caatgccgaa gaactgaaaa aacatattgg ttatttgccc gaaaacaacc ccttgtattt
     9241 ggatatgcct gttatagact atcttgagtt ttgcgcctcg ctgcaaggtg ttgaaaaatc
     9301 aaaaattccc gaacgcattc gtgagatggt tcgtgtatgt gggctgaatg ccgaaaagca
     9361 caagcgtatt ggagagttga gtaaaggctt ccgtcaacgc gtagggttgg cacaagccat
     9421 gattcataac ccatcggttt taatacttga tgaacctacc acgggccttg acccaaacca
     9481 gattattgaa attagaaagc ttatccgcga acttggccgt gaaaagacag taattttgag
     9541 tacccatatt ctttcagaag ttgaagctac ctgcgatagg attatgatta tcaataacgg
     9601 aaagattgta gctaatggta cagcggaaac tcttcgcaaa caggcacagg ggcaagaaat
     9661 tctgactgtt aaaattgaag atggcgatag taatgctata tttaaagcta ttcaggagct
     9721 accgacagtt ggtcttgttg actttaccga ccgtaacctt aacaggttcg aggtgcagag
     9781 ccgtccgggt tgtacctcaa ggcgcgaaat atttaattta tgcgtgaaaa acggctggat
     9841 acttactgaa cttgctgttt cggaagcaaa actggaagat attttccgtg aacttaccgt
     9901 gaattaagaa taaaatgaat aatacaacca cgaaatattt taaaaacatg aattctgtgt
     9961 ggattataac caaaaaagag ttgaacagct ttttcgactc gcttattgcc tacatcctgc
    10021 tggtgttatt tttaggtttt agtggctttt ttacctggat gtttggtagt gacatttttt
    10081 taattggaca ggcaacactt cgtccattct tttctattgc ctactggagc ttatttatat
    10141 ttatcccggc tcttacaatg cgtatgattg cagaagaacg taagacaggt actatcgaat
    10201 tgcttttgac aaaagccgta tctgacagac aattggtatt aggcaaattt ttagcggcat
    10261 ttttgttgat atgcatttct ttagccctta ctttgcctta tgtaattaca ttggcgtcta
    10321 taggcaatgt agataacggt gaagttattt gcggatacct tggacttgct ttaatgagtg
    10381 gggtttatat tgctattggc ctttatgcca gttctctgac gaataaccag atagttgcct
    10441 ttttgtcggc cttatttatt gggttattct ttcatattat ttttgatatt ctggccaaca
    10501 actttaaagg cagtattgga gaatttttta acatgctcag cctttctaac cattttgaaa
    10561 gcatacagcg tggagtgctt gataccaaag acctggtgta ttttctttct ttaattttcc
    10621 tgggtttatt cctaacggag aataatctgg caaaaagaaa ggtgaatggt taatgtgtaa
    10681 tattttgtaa aatccaaaat agtaatgtaa tgcaacaccc atgatagctc aggcacaaac
    10741 acgaaaatat ttattgttgg gagcaagcta tcattcattt aaaaaaatta aaaataacta
    10801 tgatgaaaaa taagatgaaa aataaatgga taacatcagt tgtgctaatt actgcaatag
    10861 tactggtagc caatcttatt tcgcaagact tttttcttcg tgtcgatttt agcgaagaca
    10921 aacaatacac cctaagttgg gcaactaaag atttattgaa aaatttgcac gaaccaatca
    10981 cagtaaaggc ctatttttct gaaaatgtac cgcccaatgt tgcaaaagtc cgcaaagact
    11041 ttaaagaaat gcttgttgaa tacaacaacc gctcaaaagg catggtggta tatgagtttg
    11101 ttgatccttc agccaaagaa gatattgaac aagaagcaac tcaggaaggg attcaacctg
    11161 tgatgattga tgttcgtgaa aaagaccaga tgaaacagca aaaagcatat ttgggcgcta
    11221 taatttcaat gggcgacaga aaagaagtaa tacctgttat tcagcctggt gcagctttag
    11281 aatatacact atctaaagct attaagaaac tttctgtagt tgaaaagcca tcagttggca
    11341 tattacaagg acatggagag ccacaaatac aagaattggc gcaggtttat gcagagctta
    11401 gcgttcttta ccaggttgag cccttaacat taaacgatag tgcagccatc cccgaaagaa
    11461 ttaagacaat tgctattgtt aggccaaccg attctattaa gcaaagccac tttgcacaat
    11521 tggatgcatt tttggcacgt ggcggaaaaa ttttagtagc agcaagcaat gtaaacgcta
    11581 accttcagca ggctattgct tctgcatctg ccgctggaat tgatcaatgg cttaaaacaa
    11641 aagggattct tttaaatcaa aatattgtta ttgatgcttc ctgctcgcaa attcaggttg
    11701 tacagaaaaa tggcgcattt cagatgatac aacaaataca attcccttac atccctgtaa
    11761 ttaaaacttt ttcaaaaacc tcaattggca gtggcctgga agctgtaata ctgccttttg
    11821 caagcactat tgagtacaat ggttcacctg ccaatattta tactccgttg gcttatacct
    11881 ctgataaatc aaacattgaa caattaccag tattttttaa tatccagaga atttggcatg
    11941 aaagtgattt tccaaaacat caacttgttg tagctgccac tttggaaggg aaattggctg
    12001 gggatcaacg ttcaaaactt gtaattattg gcgatgggga ttttgttatc aacgggaaac
    12061 aaggcgaaca acaaaaactt tctcctgata atatcaatat gtttgtaaac agtatcgatt
    12121 ggctttcaga tgacactggc cttattgagc tccggacaaa atctgtaaca gcaaggcctt
    12181 tggatgaact gggtgatgga accagaacaa ccctgaaatg gctcaatttc ttgttgccca
    12241 ttttactcat tggggcttat gggatttggc gagctcaagc taaacgtatt caacgaatta
    12301 aaagaatgga ggaacgatat gtctaataaa attaataata aaaccttgct cattgtattg
    12361 gcagggctca taatagtatt tgtcctggtc aaatggtacc agaaaaccca gacagaaagt
    12421 acattaaata ccgctttggt caaaattgac actacaaagg ttagtagaat tgctatttac
    12481 cctgttgctg aaaacagaca ggaaatttta ttttctaaaa ttggcaaaga ttggaaggtt
    12541 tcaaaaggca atataactgc cgaggttgac gaaaatgtga taaataacct tatcattact
    12601 ctttctgaga taaaagtaaa aagcctcgtg gcaaaaaata aagacaaatg ggacgatttt
    12661 aaggttagtg atactactgc aacacgcgtt aaagtgtttg aaggtaatga tgtcgtagca
    12721 gacctgatga ttgggcgttt tacatatcag caaagtcaaa atccttatgg aggaatgtat
    12781 gggggtggcg ttattggctc tacttatgtg aggcctgcag atgaagatga agtgtacgcc
    12841 gttgacggat tcctgacgtt tacatttaac cagcagttta ctgcattccg taaacaaacg
    12901 cttgcccgat ttgaaaccaa caaaatagaa aaaatcacgt ttaaataccc tgcagatagc
    12961 agctatacac tggaattgaa agataaaaaa tggtttattg gcgataaaat ggcagattca
    13021 gcaaaagttg cttcttatat cagtgccctt tcttataaaa atgcctcttc atttgtcgat
    13081 aaccagtctg cccttaatgc gcctgcatgg ctagtcagag tagaaggaaa agatatgcag
    13141 cctgttagta ttgaagcaag cagtgatggt tcaaaattta ttataaattc aacccaaaac
    13201 cccaaatcat attttgaaag tgccaaaaat aaactgtttg atgagatatt taaacctcaa
    13261 tcatctttct ttgccacgaa agaaaaaaag aaaaaataat ctgaaagtaa attttaacag
    13321 gcctaacagt tttttatctt gttaggcctg ttttttaata aagtacatta tctattagcc
    13381 gtatttttcc agcaaatact gcaatgcagc caacaacatc tttgctgtct tcccaatttt
    13441 taataggttg aagggtgtat ctgtctgaga gttcaaaata ctctaccttt aaaagttcgt
    13501 tattgttgat ttgtccgata acccattgtt ttagctcgtc caccttttta gagcctttta
    13561 atttcttcga ttcaaagatg atttttgaaa ttaacggcgc tgcagcccta tgttcgggag
    13621 ttagcagctt gttgcgcgaa ctcatagcca aaccgtcagg ctcgcgtaca ataggacagg
    13681 gtattatttg tatgtcatat ttcaactgct ttacaaaata ctgtataatg gcaagctgct
    13741 gaaaatcttt ctcgccaaag taagctttgt gcggaacaac aatatcaaat agtttggtaa
    13801 ctacctgtgc tactccatta aagtgcccgg gacggtgttt accttccatt actttgtcaa
    13861 gctggccgaa ttcaaacacg cgggtatctt tttctgggta catctcgttt acttccggat
    13921 gaaagataat gtcgcatttt gctttctcga gcatagctgt atcggcttct aatgttcggg
    13981 gataacgctc aaggtctttc gggtcattga attgcgtagg gtttacaaaa atactgacca
    14041 ctactacatc attttctgaa cggcattgcg aaacaagtga aatgtgacct tcatgcaatg
    14101 ctcccatggt tggtacaaac ccaatggttt tgccttcttc ccggtattga agcatctggc
    14161 tatgaagctc tttggatgat ttaatgattt gcatattaat tactaattac gaatgacaaa
    14221 tgactgatta aaggattgca gaaagtaaat gcttctccaa tctatttcta ctgaagcata
    14281 aagtgtgcaa tctttcccct cctttataag gagagcttgt cccgattgag gcgggaggtt
    14341 ggggtggttt atttgctatt ctggcagtca tacatttcag cttttcaacc ggtaaaggtc
    14401 aggcaaattg cggccaaagc cattgtaatc aagcccatag ccgatgacaa agtcgttcgg
    14461 gatttccgtg ccaagataat cgatgtgaac aggcatttta caagcctttg gtttgaaaaa
    14521 cattgatgct acctttattt tggcaggctg ttgttgctca atctgcttta ataaatattc
    14581 cagcgttatg ccagtatcaa taatatcttc aactataaca actgtcttgc ccttaaggtc
    14641 gatattgagc ccaataattt gttttacttc accgcttgat ttgtcagctt ggtatgaggc
    14701 aagtttgata aaggatatag ttacatgaaa atttatttcc cttatcaaat ctgccataaa
    14761 gataaacgac ccgttaagta ccccaataaa aacaacgtct tcattacaaa gttcattgtt
    14821 tatcttttgg gcaacatgct ctacaagcaa tgtaatcttg tcttttgata tgtatgggat
    14881 aaattctttg ttgtcgatgg agatgctttg cataccctga ttttttgcaa aagtaaggca
    14941 gtttggaata cttcacttgt tttaaagtcc ttttttgatt gaaaatgttt gataaatacg
    15001 gattttgaag tttaaacaat tatttttcaa ctgcaaacag caaatccgca taattattta
    15061 ttgttttgta aatcaaaaat tcaggaatga aaacggtatt ttcagagata tttaaaggct
    15121 tatttcacaa agcattaagc acggcttggt ggatgattaa aatcatgttg cccgtttcgt
    15181 ttgtagtgat gcttatgcag tattttgggt ggctcgaagt tattgcttct gtttttaagc
    15241 cggtattctc gtttattggg gtaggaggag agggtgcact tgtatttatc accagtattt
    15301 tactcaatat ttatgcagct attgcggtta ttggtactat tggttttggt atccgcgaga
    15361 taaccatttt ggctatcatg tgccttatag cccataacct gattatcgaa acggcgatac
    15421 aaaaaaagac aggctcgcaa gctgcgcgca tggtcatatt gcgtatttct atggctatta
    15481 ttgccggaat tcttttcaat tggttattgc ctgcatcaac atatgttcaa catattgtta
    15541 ctaaggcagg gcaacatctt acccttacac aagtcatgaa aaactgggtg tttgactcgg
    15601 tattcctttc tttcaaagtg atagggatta ttattggcct gatgtttata caacgtttcc
    15661 tggaatatat gggcatcatg caaaagcttt cagcagcttt caggccgttg atgaaacctt
    15721 ttggattgtc cgaaaatact tcatttcatt ggcttattgc caatgtagta ggccttactt
    15781 atggctcagc tataatgata gagcaagtag agtcgggcaa actgaaactt cgcgatgcag
    15841 acctgctcaa tcatcatgtt ggtattagcc attcattact tgaagatacg cttttgttcg
    15901 ttgctattgg ggtttcagcc tggtggattg tttttccgcg tttaatcctt gctatgtttg
    15961 ctgtttggat atataaagca gaacttttct ttttacaaaa aaaaaacgaa attagctcag
    16021 taaaataatc atgcaatgaa gcagtattta ggccttttac aacacgtttt agataacggg
    16081 acaaaaaaag atgaccgcac aggtacaggt actatcagca tttttggata ccagatgagg
    16141 tttgaccttg gcgagggatt ccctttactc acaaccaaaa agcttcatac ccgctctatc
    16201 atatacgagt tgctttggtt ccttcgtggc gaaacaaaca tcaggtttct taaagataac
    16261 aatgtttcta tttgggacga atgggctgat aaaaacggag accttgggcc tgtttacgga
    16321 aaacaatggc gttcgtggca aacggcagat ggccggacaa tagaccaaat atcacagctt
    16381 atagatatga tcaaaacgaa cccggactca cgacgactga ttgttagcgc ctggaatcct
    16441 ggagacattg ataaaatggc tcttccgcca tgtcatacca tgtttcagtt ttacgtggct
    16501 aatggcaagc tctcgtgcca gctttatcag cgtagtgcag atatttttct tggagttccg
    16561 tttaatatag catcgtatgc cttgttaacc atgatgatag ctcaagtgtg caacctaaaa
    16621 cccggtgagt ttgtgcatac ttttggtgat gcccatattt atctcaatca tttagagcaa
    16681 gtcaaactgc agttgtcccg agaaccgagg ccattgccta caatgaagat tgtcagaaat
    16741 gtaaatagta tttttgactt taaatacgaa gactttctgc ttgaaggtta tgacccgcat
    16801 ccacatatca aaggcgaggt agcggtgtaa aataacccca aaaaactaaa cagaagctat
    16861 tgtttttgtt gcattgccca acatcaactc ttctgtttga tggtgcaaaa tattggcaac
    16921 attaaaaatt cactgaaagg aactaaaaaa taaaaaaagc tcccacaaat attcagggag
    16981 cttttcctaa caaatgttat ataaaagtaa ttgggctcaa ccacatttcg agttgccaca
    17041 gtttttgcac gtgaggcaac cttcttgata tactaaactt tctgaaccac aagctccgca
    17101 tttttgacca gcttttgcct gtgtgccatc cgggataaat ttcttcaaag cacgttcaac
    17161 gccatttttc caagtattga ttgtttcgct atcaagctgc aatgaactaa ccaagttgac
    17221 caactcagga ataggcatgc cataacgcaa tacactcgat ataagtttgg cataattcca
    17281 atattcttta ttaaactggt gagacaaacc ccctaaggtg tttttatagc catatttgtc
    17341 ggtgtattgg aagtcataac gggtattccc atcctcatct ttattcttga tgatctttcc
    17401 ttttgtaatt gattttggaa tcgggaaaat atcctcatcc tgcaaacctg taaatatctc
    17461 ataaggctga ccatccaaaa tgcccacaaa tgcaatccag ttttcgttgt tgtttttgaa
    17521 acgaacaatc tctgcatcta atactttagg acgttttatt ggttcagaaa aactttcttt
    17581 cttttctttt ttggcattgt ttgaaataag caccccggca cgtgaatttt cgcgatacac
    17641 tgtacaaccc ttgcaaccgc tctgccatgc tttgatatac aaatcgctca caagttgttc
    17701 atctacattc tcgggcaggt tgatggtaac actgatagag tggtcaaccc atttttggat
    17761 ttcgccttgc atgtgtacct tttgcagcca gtcaacatct gcagaggttg acttataata
    17821 aggcgatttg gctaccaact cattaagttg ttcctgatca tattttttta cctcttcaac
    17881 attatatccg tttacctcaa gccatgtaag gaatttatga tgaaatacaa catattcttc
    17941 ccaatggtcg ccaacttcat cagtaaaaac tattttaaca ttagtatcat tagggtttac
    18001 tttacgacga cgtttgtaaa caggtaaaaa aacaggttca ataccagatg ttgtctgtgt
    18061 catcatactg gtagtgccag ttggtgcaat agtcaaaagg gctatatttc gacgcccaaa
    18121 gcgtaccata tcttggtaca acttttcatc ttctttacga agcctgttga taaacggatt
    18181 gttcttttcg agttgtgaat catagatact gaaagggcct cgttctttgg ccaaattaac
    18241 agaagaacga taagcttcca atgccagggt acgatgtact tctaccgaaa aatctattgc
    18301 attgtcgctc ccataacgga tgccaagagc agccaacata tcaccttcag cagtaatgcc
    18361 taccccggta cgacggcctt cctcggtctt aatacgaatg ctttcccaaa gtttcttttc
    18421 aacacgtttt acttcaaaat cttcaggatc tgcatctatt tttctcagga tagcatctat
    18481 tttctctatt tctaagtcga taatatcgtc catgaaacgt tgagcaacag ctacatgtcg
    18541 cttgaacagg tcaaaattga aacgggcctg cggagtgaaa ggattttcaa catatccata
    18601 aagattgata gctaaaagcc tgcagctgtc ataaggacac agaggtatct caccacaggg
    18661 gttggtagaa acagttttat acccaaattg tgaataacaa tcaggcacag actcgcgtat
    18721 aatagtatcc caaaataaaa tcccgggttc tgcagatttc catgcattat gcacgatctt
    18781 tttccaaata gcacgggcat ctactttctt tgtaaatgaa ggactggccg aatcaacagg
    18841 atattgttga atatattcag tgccatctat gacagcgcgc atgaaatcat catcaatctt
    18901 aaccgataca ttggccccgg ttaccttccc ctgttcaagc tttgcatcaa taaatttttc
    18961 ggcatcgggg tgtttgctgg aaatagacaa catcaatgcc cctctacggc catcttgtgc
    19021 aacctcacgg gttgagtttg aatatctttc catgaatgga acaactccag ttgaagtgag
    19081 tgcgctgttc aatacagggc tgccagaagg acggacatgc gacaggtcgt gccctacacc
    19141 gccacggcgt ttcatcaact ggacctgctc ctggtctgtc ttcatgatac ctccataaga
    19201 gtctgcgcta tttccaatga caaaacagtt agataatgaa gcaatctgaa agttattgcc
    19261 aatacctgtc atagggctgc cctgaggtac aatatactta aaatctttca aatattcata
    19321 tacttcctct tcactcatag gattggcata ctttgcctca atgcgcgcaa tttccttggc
    19381 aatacgccgg tgcatatggt cagggttaag ctcatagata ttcccaaacg aatcttttaa
    19441 tgcatatttg ttaatccaca cttgtgcagc catctcatcg cctttaaaat acgcaaccga
    19501 gctttcgaag gcttctttat atgtaaatgt acctggaact tttgtttgtt ttggtgtttt
    19561 gctcattgta tcgtcaactt gtttaatttc ggagacaggt gttaggtcgt tttgcatggt
    19621 taggtaatat taatctgtta attgaacggg ttttacaaaa aatgtcagcg tttaattttc
    19681 attgtgcaat ttacattctg aaattctttg cttccaaaaa aaccacctca cagttattaa
    19741 ctgataaaat gtagttatta acattgacaa aaatcatatt tttacatttg ctaaattgtt
    19801 aatattcaaa atattgcaat taacaactgt aataaaacac tgacattcat cacaatttga
    19861 tttttatata aaaaatttta tacctttatt aaatgtatta ttacctaaac ttaatttcca
    19921 taatcatgaa acgagcatga ttagttaaac acaaacaagg atgaatcccg atttgtcaga
    19981 acgggttttt tcttcggata gcctttcgct gggttcatcc ggccttatta aaaaaaatta
    20041 attacagatg aaaagtttat tatttctggt atttgcatta ggttgtaatc ttattttagg
    20101 ccaaaaagta atatttcttc accactcaac tggcggggga gtttataatg aaggcagcgt
    20161 tgcacaatgg tttgacacat acaatagtca aaactccaca aactatgaaa ttacagaacg
    20221 tgcatatccc gatagcccat atccttggga aaattatcca tacgattatt ggaatttatg
    20281 gattaatgga gcatgtaaca atgataattc cggtaccgaa tgcattaact cgattgtaac
    20341 aaaatacgat gtaatcatct tcaaacattg cttcccggga gcaggtattg gcgaagacga
    20401 agctgaaaat agcgtttcat catcatataa gtcaatgggc aattataaat tgcaataccg
    20461 cgctttacga actttgatgg acagtttccc caacaacaaa ttcatagttt ggacacttgc
    20521 cccgttgcac cgaaatgcta ccgattcggc ttcagcagca cgggcaaaac aatttgttga
    20581 ctgggttaaa taccagtggc ttaaagaaga tagtaaatcg cacccaaata tttttgtttt
    20641 tgacttttgg ggatatgtag cagaaactaa tgccaatccc agccagggaa aagttaattg
    20701 cctgaagtac gattatgaag gcagtcatga cggagatgat tctcatccca atacccaggc
    20761 caatcagact gttggcccaa tttttgggca attcatagtt gattgtataa aacaaactaa
    20821 tacccgcata attaataaaa cactggacaa cattactgtt ttttataaca atggctgttt
    20881 ggcaattcaa ggattaaatg aaattgttcc tcaatctgac ttgtgtatat atagcataga
    20941 tggacatttg ctatacaaaa aacggattca aagcagtata gctctgcctt tatcagaggg
    21001 gatgtatctt gtatccattc acaacaaaac atttggaacc caaacggcaa aattctgtgt
    21061 gataaaataa cctccaataa atttcgtttc ttttacagtt tttaaaacat atcataatta
    21121 cggtaatcaa aagctgccta tctttgttgc tataaaactt aaaaggatga gagtacactt
    21181 cattgctata ggaggcagcg ccatgcatag cctggctctt gcactgcacg aaaaaggata
    21241 tattgttaca ggttcagatg acgacttttt tgaaccatca aaaagtaagc ttgaaaaagc
    21301 aggattactg ccaaaagaaa aaggctggga tgcatcgcgg gttacccctg aattggacgc
    21361 tgtaatattg ggtatgcatg ccagggacaa caatcccgaa ctggcaaaag cccgtgaact
    21421 agggcttaaa atattctctt atcctgaata cctttatgaa caaactaaga acaagacacg
    21481 tgttgtaatc ggtggtagtc atggcaaaac aactatcacc tcgatgatta tccatgtact
    21541 cagctactgg aagaagtcgt tcgactatat ggtcggtgca cagctcgaag gctacaataa
    21601 catggtgcgt ttatcagaag attcgcctgt tgcagtgttc gaaggagacg aatatctttc
    21661 ttcaacgctc gatccaaggc ctaagtttca tatatatcag ccaaatatag ctgtaatcaa
    21721 tggtatagcc tgggatcata tcaacgtgtt ttctactttt gatgtttatg tagaacaatt
    21781 caaaattttt atcgaaaaga tacaatcaaa aggcactctt atctactgtg cagatgaccc
    21841 tcttgttgca ggcattgcta tgactgctgc accggatgtg aaaaaagtac cttacaaaat
    21901 ccatgaacac caagtcataa gcaacgaggt tttcttaata aatgacaaaa acaaaaccag
    21961 agtgcatgtt ttcggcaaac ataacatgca aaacctttcg gccgctaaag ctgtatgcaa
    22021 cgagttaggg ttgaccaacg agcagtttta cgaagctatt gctacattca aaggtgctgc
    22081 aaaacgcctt cagttgttaa gcgaaaataa catgaccagt atttacctgg attttgcaca
    22141 ctctccatca aaattgacag caacagttag ggctgtaaaa gaacattacc ctgaaagggc
    22201 tttggtggct tgtatggagc ttcatacctt tagcagcctt tctgaagatt tccttcgtca
    22261 atactatggc accatggaaa gggccgatta tgccattgtt tactacagca tgaaagccct
    22321 tgcggccaaa ggcttgaaac cgcttgaccc ggaagatgtg gctaaggctt ttgcaactaa
    22381 acacctgaaa gtatttaccg attctgatca actggtcaag caccttaaaa gtattgaatg
    22441 gaaaggtaaa aacctgttac tgatgagttc tggtaacttt gacggcatca acttcaatac
    22501 ctttgcaaaa gaacttttaa accagaaata ataattgttt actaatatgt ttaattttaa
    22561 agatatgaga aaacttgtct ttttattctt cattattttt attgctttca caaacatcaa
    22621 agctcagcag gctataggag cccgtctttc tgacaacttt ggcattacct ataaatatac
    22681 ctatagcaat cctgttaaac tcgaagggat tttagaaatt caccgccatg ctttaaccgt
    22741 aaacggtctt tacgaaaggt actaccaaac ccctttttct aagtatttcg actggtattt
    22801 tgggggaggc gcttttataa acttctggag cgtcaatgac aatggcggtg tcaatgtggg
    22861 gttaagaggc gttgctggta tttgctatac atttagcgat atccctattg acatttcgtt
    22921 agactggatg ccagctatac agattgtaag tgattatcat gcagactttg ccatattggg
    22981 actttcagtt cgttatgttt tttgataaaa atttttgaaa aaatattttg gatattaaaa
    23041 atatttctac ctttgcaccg cttttaacga tggtccgttc atctaagggt taggattcaa
    23101 gattttcatt cttgccatag gggttcgaat cccctacgga ctacaaaaaa gattaagctg
    23161 cagtttttaa gttgcagctt tttttgtttt aatactttct tttcatcata tttaattttt
    23221 tatttttaaa ggaataatag cttgtcccca ccaataaata ttttcgtgtt tgggattgag
    23281 taattatgga tgtttcattt acttcatagt atatttttgg gatacataaa aaaattttaa
    23341 tgcgaatttc agccttaatt ttactatgct tcgttgccca atttggttat gcccaaaaac
    23401 gcatctcagg ttttttgact gatgcaaatg gtgtcccgct cgaaaacgct tctattttcc
    23461 tgaaaggggg cagattatta actactacag acaaaaacgg ggctttttca ttcgaatata
    23521 aacaagacga aagtatatgt ttttcgcatt ttggtttttc cgacacttgt ctcaatgctt
    23581 ctgaactatc agagaattca ataaaaatca ggctgaacga aaaaaagatt gtggccgaag
    23641 aaatcattgt gtcatcaaag tcgaacatga aaatcatcag gtctgcacag acaggtctgt
    23701 tcgagattcg cacagacagc cttcgcagcc tgcctaatat tgttggagaa gctgatccaa
    23761 tcaaaatgtt gcaattgtcg ccgggggtga caaaaagcga tttcaacatg gggctcaatg
    23821 tccgtggcag tactactgac caaaaccttg tcctgattga cgatgctgcc atttacaacc
    23881 ctactcacct ggctggcttt ctatctgtct ttaaccctct tgtagttgaa aaagtatcgc
    23941 tattgaaatc cggcattcct gctgaatacg gaggacgggt ttcttctata accattgtag
    24001 aaacagataa gaacctggct tctaaaactg aaacagaagg caatgttgga atacttttat
    24061 cgggctttgt tgtaaaaacg cctttgttac aagacaaagc ctcatttcag gtttctgcaa
    24121 ggcgttctta ctttgaccaa accataaaac caataagtaa aatccttttt aaaaatggga
    24181 agtcgatgtt caactccacg gattatgcat tttacgatgt aaatgccagc ctgattattg
    24241 caccctcaaa gaatgacaag gtgtttatca gttgttatgt cggaaatgat gacttttcgc
    24301 taagcaaaac ttcattcaat ctgcaatata atatgcaatg gagcaaccag gctttttcgc
    24361 taaaatggat gcaccatttc agctcccgtc atatcatgaa aaccattgct tatagttcca
    24421 aaggagactt cgcaatcttt ctggggcaaa acgactttaa ttttcgcctc aattcttcca
    24481 ttgaagactt atcagctatc cacgagcata ctttcttttt tgaacattgg agcctgaaaa
    24541 ccggggcaca ggttttgaaa caacacattc tgcccaacaa aagcaaggcc gagctcattg
    24601 atttcaatac cgattttggc acgcctaata ctttcaacac gttgacgttt agcccttaca
    24661 ttcagtcgga agtgaatgtc tcaaaaagct tggcactaaa cattggcctc cgttacaatg
    24721 aatatctcca cttcggtcct tttgaacgat ttgtgcggga caatggcatt gacattaccg
    24781 acactttata ttacaaaaaa aatgaaacgg taaagaaata taacaatttt gaacctcgat
    24841 ttgccttaag gtacttaatt aactcatatt cgtcgttaaa attttctgta actcataata
    24901 tacaatattt acagcaaatt aacgtttctg cagtttcgtt ccctactgac ttttggatgc
    24961 ctgcttcata tcaattagcc ccgcaaaaag gttggcaatt ttcattgggc tatttttcca
    25021 acctgtttga ttccagttat tgcttttcca ctgaagtgtt ttataaacac atggtaaacc
    25081 ttacagaatt caaaaaaggg attcttagta ctgtttcaaa ggcctctatg gaagaaaata
    25141 tcattaccgg cgagggacgt gcttacggga tagagtttct cattgagaag aagaccgggg
    25201 aactgaccgg atggataagt tatactttgt cgcgttccga aagaattttt aaagaaatca
    25261 acaatggtaa cccttaccct tcaaaatacg accgtaccca cgacctttct attgttacac
    25321 aatataaaat caatgaaaaa tggaacgcct cggccatatt tgtcattgcc agcgggaatg
    25381 ctttcaccct gccttatgga cgctatatga taggcgaaaa tattgtcaat caatattccg
    25441 attacaattc gtttcgcatg ccggtatatc acagattgga tatgtcggtt acccgcaagt
    25501 ttaaaacaaa aggtaaagct gaacatagcc ttgatattgg catttacaat gtttacagcc
    25561 ggttaaaccc ttatttcatg tatttcgacg tttcgggcga tttggaaaaa tacaaattga
    25621 aagttagtcc gcggtatgta tcagtgttcc ccatactacc gtctgtttct tatcatttta
    25681 aattttaata cttaaagaaa atgagcatag aaatgagcat acaagaaata aagccattgg
    25741 ataaactaag aagtgtttta aaaacaagct tcattaaaag catgacaatt gctatgtgtt
    25801 gcttaacctg cattgcctgc gaaccagaaa tggactatga acttacatca gtaaaaaagc
    25861 agcttgtagt agatggatgg atagaaaaca ataattatgc ccaagttgtt ttgacatata
    25921 acactggtta ctttagcaat ctcgactccg cctcattcag agggttggtt gcttcaaggg
    25981 ccaaggtaat ccttagcgat ggtactcaat ccgaaattct tacgctgaca aaagatacca
    26041 actatttccc tccttacgtt tacaaaggga acgaaatact tggccaaccg ggaaaaactt
    26101 atactttgat tattatagat gagattaata ccggcacaag agaaatggat accattgtag
    26161 ctattactac tataccccag cctgtaaagc ttgatagcgt gtggttttca gaaacatctg
    26221 atagctcagg tattataaaa ggcattatat ttgacaaccc ggatgaaaaa aactattatc
    26281 gtactttttc taaaataaaa ggaaaagaca aacgcttcat cccaacctac attgcttctt
    26341 ttgaggacaa atacttcaat ggacaaaagt tcacctttca tctcaacaaa gggcttgatt
    26401 cgtatataaa accattgtac aatatagaat ttaataaagg tgataccata aaccttaaaa
    26461 ttgcaagtat tgacagaata agtttcttgt tctggagtag ttatgaccag gaaatcttca
    26521 attcgggtaa cccttttgct tcaaattttg atagagtaaa tagcaatatc catgggggat
    26581 taggtatttg gtgtggatat ggcgctacaa catatttggt ggttgctaaa taaaaaatgg
    26641 ctacccaaag gcagccattt tcaatatttt gttgttgata actaatttat atggttaatt
    26701 ttccagttat aagttttatt taagtcatct ataaaactgt taaaactggt tattaaattg
    26761 tcaaagcctt ggttgaaata ggtttccatg cttgactttg agccatcggc aaataccatc
    26821 tgcacatcta tcacttttct ggttttttga acagttgaac aattgtattc ccaagtattt
    26881 gcatcccagg tacagttata ttcatcatat ttttcatttt ttgcataagc ctcagcttta
    26941 gcaacagctt tattatcgct ggcataaact actaccaatg ccatattctt attaatcatt
    27001 gaaacagcgc tatcaatata agctgaatat ttattttctt caatcttgcg gtattctttc
    27061 atgaaattag caaaatcgat ttgcccaaca attttcatat ccatgaactg gaaatgagca
    27121 ttggcgttgt acaaaactct ttcagggaaa gtttctgttt ctttgtactc ataaatattt
    27181 tgaatagttt cgtactgccc tgtttcatag ttgtaatagg tatagcttga ggtgtctttt
    27241 attgtttttg tttcctgggc attgtcaata gctgctttgg taaacttacc cccggcggtt
    27301 ccgcccaaat ctaaaattgt agtacttccg tgtgtaaatg aataatttac agtcacttct
    27361 gtttctttat acccataagt aagtttaagt tcaaaagcgc ctaaagtgag cttgccttca
    27421 acatttgagg gaagtccttc atctgtgtag cttgcagtaa aactaaagct tgaaacaaca
    27481 gtgccatcaa cacttaattc atatttaagc tcagaaggca tctcttttgt atcgacaaga
    27541 gtaaagttgc cggtaacaaa tttgggcttg ctaataacta atttgccatc attgtttttg
    27601 gcctttttag acgctataga agccggaaat ttaaaaacaa cttgtcctga gctggcattg
    27661 gcagcaaaat caaaatcatt tttggtatag ttataagtgt aaaccccggc aattttatca
    27721 aattctgctt ctaattctaa tgaattagaa gtttcagctt taagcgattt aaaaataaca
    27781 tttgcatcag ggttattgct actaatattt ttcacagctg ccataacctg aaagtatgca
    27841 gaattcttga tgcttccatt taccccagtt cctatattca tcagctcaag aagattaaca
    27901 gtggctttaa ccccttggtc ttgattcata gccttaagct cattgaccat ctttatgccg
    27961 gtattttcca tgtcaacctt ggtttcggct acagtttttt cggcatacac aggtttttct
    28021 aaatttactt cttctgttgt tttgtcatca tctttgctac atgaagaaat gatggacgca
    28081 cacattgcaa gaaacaaaat tttgattttc atactattta ttttttaaag gttatttact
    28141 tcaaagataa ccatttcata taaaagttaa aaaatcaaaa accatatttt tttataacag
    28201 ctgttttatt tttcatcgca caaaacgcct tttcttactg tttttctaat tagattaatg
    28261 attgtaaacc gggttgaacc ttaaatatgc gcttacaaaa cccagaaaca atatctttgc
    28321 catgcataaa ttaaaccatg aacacatgaa gaaacttacc ttatttatag ctattgctat
    28381 aacctgtttt tcagttaaag cacagtatag tcaggatgta atattatcat caattctaaa
    28441 aacggacacc acttctatcg ggcaaaagat tatatttccg caaaacaata ataacgaaat
    28501 ttctattatt aaagtaacca taccgcccgg aaaatcaacc ggatggcaca aacacagcaa
    28561 cccggttttt gcctatgtta taagtggcac gttaaccgtt gaacttgaag gggataaaaa
    28621 gcttacattc tcagccaata cctcttttgc cgaggtcatc aatacatatc acaatggcac
    28681 aaaccttggg aaagaagagg ttgtccttat cgcctttttt atgggagaaa agggtaaact
    28741 tttatctgaa cacaaatagt actgcatact accatttttc tttcaaaaga tcgggcagga
    28801 tgtattgatt atacaccttt tcaattgcct gcagttgttc ttggtccaga ttgggcaatt
    28861 cacaagcttt caggtttgac aacacctgct ctggctttga agcgccggga attacacagc
    28921 taacctcacg aaacatgata atccatcgca atgcaataag ggcaaggtca gcattagcag
    28981 gaaatatctt tttcagctca tctactgccg ccaaaccctt ttcataattg atgcccgaga
    29041 atgtctcccc tttgtcgaag gcttcgccat tgcgattaaa tgtgcgatgg tcgtcttttc
    29101 caaaaacagt agccttgctg tatttaccgg tcagtaagcc actggcaagc ggaacacgga
    29161 caatgatgcc cacgttcttt ttagctgctt cggcaaagaa atcttttgcc ggacgctggc
    29221 gaaacatatt gaaaattatc tggactgtag taacattgtc atattcaata gcctttagcg
    29281 cctcttctac tctttcgacg cttactccca tatttaaaat tttaccttct tttttcagct
    29341 catcaaacaa agcaaatatc tcaggacggt gataagtctc tgtaggcgga caatgcaatt
    29401 ggatgaggtc aatggcttca agtccggtat ttttaaggct atcttcaaca aactttcgaa
    29461 gcacagcagg ttgatatgca gcatcaacgt gaggcgaaag tttgcggcca catttagtag
    29521 caacaaatac ccgttctttt cgtgaacgaa ctacgcggcc tactgctttt tcgctttcgc
    29581 catcaccata aacatcggca gtgtcaataa aattaatccc gttatcaata gccaggttca
    29641 atatcctgtc ggcattttca tggctaaatt tatcgcccca tttaccgcca acttgccatg
    29701 taccgaggct tatttcggat atattaaaac ctgttttccc aagtattctt ttattcataa
    29761 tcgaatttta aaaattcatg acaatccata tcgtgcaaaa atagtaacag aaagcaagac
    29821 ttttatctaa acaaaattat ttttcaaaga atgatagctt gccatatctt tgaagagcga
    29881 atatctaatg taatatttct ttttacacat cctcgtttgt gtttgtagta acatgggggc
    29941 gttcataata aattcttaaa tattcttatc aagttaatag ctaacaaaag taaattacta
    30001 tttttacttt gctaatttta ctgttgcaca aaaaaattcg accatgaaga ggaatatttg
    30061 tatgaaagct ttctattcaa ttgttcaaaa gattgttttt ggagcgatag cttttacagt
    30121 gttaagcata aaccaggaag ccaaatcgca gaacatcaaa cagtttcaag gtgcttatga
    30181 aaatggaact gcaacttacc aatattatga aaatgctaat tacgaacgca tctatcaggg
    30241 tagtttcaaa tacaaaggta ttgtaatgga cggtttgaaa ggtaagctaa accttactgt
    30301 tgtaggtcaa tacaataaca acaaaaaaga cgggctgtgg acatatactc tggccgatcc
    30361 ttcacctacc ggggttacag aagtagtaac tggcaactat tccaatggca acatggaagg
    30421 cgaatggtct tctactacta aagtcaataa tacaaagaaa gttttcaaaa gtacttctgc
    30481 tatttttaag aacaataaac tttttggcga aatcaattat acttataaag tttacacttt
    30541 caaagactat gccgccattt ctattaaagg ctcgttcaac gattccagtt tttacaatgg
    30601 cacatggctt accacttata caaaaggcaa tatacagtac gaggaaaccc gcaaatacaa
    30661 caacggagta ctctgtttac ttgtacaccg aaggctttct gacggtcaga ttattgaaag
    30721 atatgacagt actgcttttg tagataagtt ttttatgaac tataatgctg ctcataatgt
    30781 tggtatggtt aatgaccaaa aatatgtact tcagaatgtt gataaacctt ctggttcact
    30841 cgatttattt agcatagtag ttaactactg gacaaacaat aaaaatactg cctattataa
    30901 tacgctcaat gctcctaatc caatgtttgt tattagtcat ggctatagtc ctgctgttat
    30961 cagccgcgaa aaaacaatta tcagctggtt aaagacccgt gaaggcgagc gtcaacaatg
    31021 gcagtctgaa caggataaaa aactattgca ggaaaaatat gcagcccagc ttgctattgc
    31081 agatacagcc tttaaccgtg ccaaatatga tgaagctatt gctgcttaca aggttgcttt
    31141 aaccataaaa gacgaacaat atccgaaaga ccagattcat aaagcagggc tgaagctcga
    31201 agaaattagg ctcagaatgg aaatggaaca aaaagccaaa gagcttgaac agcaagccag
    31261 ggaaaaatct tataaagaac tgctaacaaa ggctgatgcc gcttttgcag aaaaaagata
    31321 tgatgtggcc attgagctat accagagtgc ccttgccata aaagtggacg attatcctgc
    31381 caaacaaata aaagccatta atgacattaa aaacgatgaa ttaaaggcaa agcttattaa
    31441 agaacttgat aaactttggg ttacagttga agccggcact tttaagatgg gatgcatacg
    31501 aagcgatatg aattgcctga gaaacgaaga acctgtgcat gaagtttatg tcaacacttt
    31561 tcagatgagc aaatacgagg ttactattgg tcagtacaga atgtattgca aagcaaccgg
    31621 taagcctgag cctctgggag ctgatagttt gcctgccaat aatataagct ggtacgaagc
    31681 taaagagttt gccgaatggg caggatgccg cctcccaact gaagcagaat gggaatatgc
    31741 tgcccgtggc gggaacaaaa ataaaaaaac cttatatagc gggtctaatt atattgatga
    31801 agttgcttgg ttcagcgaaa actcaaatag ctcagcccat ccggttggca aactcaaacc
    31861 taacgttttg ggtatttacg atatgacagg caatgtatgg gaatggtgtt ttgactggct
    31921 tagtgattat tccgaaacca gcgacataaa ccccactggc cgttctaccg gaactcgtaa
    31981 ggtaaaaaga ggcggcagct ttagcgaaac aaactttgaa agcgacttgc gtgttactta
    32041 ccgtggtagt gaacctgctg acaccaggct atacaatctg ggaatccgat tggtcaagaa
    32101 acaataaaaa agaataaatt gtaaaaaact tagcagcttt tttgatttaa cccaaattaa
    32161 gcaataaaaa tgcttaatcg gttaaaattt tgtatttcaa tgatttaaaa aaatcagctt
    32221 gccccgttta taacgagagg attaacccaa ctaaatctta tagtttggct ttaaaaagcc
    32281 tcataatgat ttatttagcc tgttcaaggc agaacgcttg tgcaacagtt aaattacaac
    32341 gttttgtgtc gttttctggc aaacaaatgt atttaaaata taaatggtat tagtctatat
    32401 gttttttcca tgtattcctt atattcattc ccaaaatgct taagcagcat ttgttcttca
    32461 atcagtaatc gttttataaa tgaaaatgga actgttaaaa caatcaaaat tatgcttatc
    32521 agattgccag ttaataatcc tgccccaaca agaaatgtag ttacccccaa atatccaggg
    32581 tgccttacaa tgctataaat accatgttta actatcttat gtttatctgt tatttttaaa
    32641 gttcttgtgt aatactcttt tagtgtaaat gttgctatta acctaattat taagccgact
    32701 atcacaaagc ttaaaccaat aaaaaacaaa acttggctat cgatttgcaa tatattatac
    32761 aggttaagaa aataactgca taaaaccaaa ataattgttg atattaatgc aaatctaagt
    32821 attgccgtcg tacccttatc tgagtcagag gtttttaaag atttggcatc tttgctaaat
    32881 ctcaagaaat tctcaatcat cagaaatatc aaaatcgaac caatcgaaat ataaaaccct
    32941 gtcatagttg atttttttag atgtttgctg acaatggact ttgcctggtg taaaatacaa
    33001 ggctgatatt gtcataactg taaacaaata taacgaataa aaatgttttt ttgcaacatg
    33061 ttaaatttca acataatgta ttagcttata tggatgcttc cctttgtaaa attttttcta
    33121 tattttattt gccgggttca ggggtaaaac aatatgagta tagtaaatga ttgtttttct
    33181 gaattttaca gatactttta cgccgggcaa actttattta aaacttttat aaggaaatac
    33241 tattattcta tttccttact ttttcttaaa aaaaactgcc cgacttttga gaagtttttc
    33301 ataaataaag taaagcatcc gccaacctta ttacgtttca tgatgtgata atcttctgtg
    33361 ctttttctga taatattttt caggcttcgg ttacttgctc ctccaacttt catgcgggtt
    33421 ataatttgag gataatagta aatccttata cctggaagta ataacgtacg caacatcaaa
    33481 tcataatccg atgcaatgcg atattgcaaa ttgtaattcc cgatacgggc aaatatttcg
    33541 cgttttacaa agaaggtagg atgagggggc atccaaccac gcgaaagcat ttttctgtca
    33601 aaaggcaaac ttttccaatc ccggattatc ttgccttcaa aactcacata caacaggttg
    33661 ccatacaata catcgcattg ctctttttca aaagcattgg caacgtctgc aagcacctca
    33721 ttgttgtcaa aagtatcatc ggcatgtaaa attccaatca catcaccggt tgccttagat
    33781 atacctttgt ttaacgcatc atatatacct ttgtcaggtt ctgatatata aacagcaatt
    33841 ttatcgaggt gtctctctgt aatatctttt gaactatcag ttgaaccgcc atctataaca
    33901 atatgctcaa tgttagtgta ggtttgaagg gctatagaat tgagcgcctc ctctaaagtt
    33961 ttttcgctat tataaacagc agtgattatg cttattttaa ccggcatcag attctgtttt
    34021 taattttcac agctggatta cctgaatgga tagaatatgg ttcaagattt ttagttgcta
    34081 tcgaaccaac agtaaggact gcatgacttg ctacatgaac cccgggacat acaacggctt
    34141 ttgctcctat ccatgcacca tcttcaatat ttataggggc tatcattagg tcaaaggttt
    34201 cttttttgta gttatgattg ccgcaaagca ataatgctcc ttgcgaaata caaacatttt
    34261 tgccaaggtt tacctggtcg aggttatcaa tccaggcatt ttcaccaacc caggtgtaat
    34321 caccaatgct aagccgccag ggatatttga tattcacacg gggcttaatg caaacccctt
    34381 ttccaacctt ggccccaaaa agcctcaaga tagcattttt aatactgtaa gggagtgtaa
    34441 taaaagagca gaaaaaaact gcatttacaa tataccacaa ggctattacc agtttattcc
    34501 gtcccggatg ataccagcta ttgttatatt tatcaagtcg ggtatgaaac atcgatgctt
    34561 tatttgttga taaagttatt taaagtgcct aataaaacgt ctatttgaat aggcttagta
    34621 atatatccgt cacagccagc ttctatagct ttttccattt catcggccat ggcaaatgcc
    34681 gtctgggcaa ttaccggtaa ttcgggccta aggcttttaa tttctttcat agctgtgtaa
    34741 ccgtctttaa caggcatttt acaatctatc agaacgatag caatgttggc attattacgc
    34801 acagcatcta cagcttcttg accattcttg gcccaaatga tattttctgt caatccgttc
    34861 aggatatatg acaagtacaa ataattataa tcctcatctt cagcaatcag aatgacttta
    34921 tctttgggaa aagctatgtt tgtaatgttt aagttatgta tagaattagg aagaggtttt
    34981 tcgcttgatt tgccagcaaa atatggtata ttaaagtaaa aacaagcacc ttgtccgggt
    35041 tctgaatcaa cccacataga accacctaat aaacgtgtaa ggtttgaaga tattgccagc
    35101 ccaagcccgg ttcctttttg gttgataaaa aagttgctat ctatcacctg cccaaaacgc
    35161 tcgaaaataa ttttctgttt atcttttgga atgccaatac ctgtatcttt tacatgaaac
    35221 tgaatagttt tgttttcaag aaattcataa ccaaactcaa tagacccctt gaaagtaaat
    35281 tttaaggcat tgcctatcag gttagaaata atctggcgca ggcggaaagg gtctgttaca
    35341 atattgcaat cttcattggg caggcttttg gtacaaataa gctttatttc gtttttattt
    35401 tctttaatgc ggctggcctc aaatgactca aatatttcat ccatcaggtt attgaggttg
    35461 caatccaaca tcctgatttt aatctgtccg gcttcaatct tagaaatatc aataatatcg
    35521 tcaataagat tgagtaaagt attgccacag cttgtaatat ggttcaaaaa ctcttctcgt
    35581 tcaatatcgg tgaggtcagg gttgcgcagc aaattagaaa aaccaatgat cccgttcatt
    35641 ggggtacgta tttcgtggct catattggcc aaaaaagcag ttttcagcat atctgactct
    35701 tcggcctttt cttttgcttt ccgaagtcgc tcttcggcaa gccttttctc tgtaatatca
    35761 atagacatac ctactatacc gatgatgttt ccttcgcggt cttttaccgg aattttggta
    35821 gcttctaccc aaatggtatt gttttcagcg tcttttaaca gctcaagttt gctaatcatg
    35881 ggtttgccag agttcaacaa ctcctgctcg tcctgaaaag ctgtttctgc tactgtttgt
    35941 gggaaaaagt caaagtcagt tttcccaatg gcttcatttg catcctcaac acccaaaagt
    36001 tttgcctgcg ctttgtttat tttggtaaaa tgcccctggg gatctttgag atatatggtg
    36061 taagggatgt tgtccatcaa tacctgcaag aaatttcgtt cttgctcaag catcgaattg
    36121 acttttcgct tttctgaaat atcgcgtgaa atacctatca atccttcaat cttgccattt
    36181 tcgttaaaaa ttgggctttt tgaagtagaa taccaacggc ttgtaccatc tggctgtgta
    36241 agcaattctt ccttacctaa tatagactgc cctgtttcaa tgactttttt atcgtcaatg
    36301 tcttgttctt tggcataagc ctcagaataa aaatctgcat ctgtttttcc gtgggccatt
    36361 tgagtctcat caagccccat attaagagct tttgccttgt ttatacgaac atatctccct
    36421 gaggtgtctt taaaataaat taaatcaggg ctattgtcca tcaaagagtg catcaggtca
    36481 cgttcatgtt tgagcgagtt gagcgtttct atattatcgc tgatatttcg ctcaaagcca
    36541 aaggtgccaa taacttctcc ttctgagttg atgataggcg atttgaccgt tgcataccag
    36601 ccattctctc ttcgggtttg aaaatgtata gtttgttttt tttcaataac ctttaagtct
    36661 tcctggtgaa aagtatttgc aatttcgtat ggtaaaaagt caaatacggt tttgcccacc
    36721 atatttgctt ttgaaatacc tgctatatcc tcataaatgg tattagcata gatgaacttg
    36781 ccatctttat ctttgagcca tgccgcaaat ggaatactat cgagtattgc attacgttcg
    36841 accagtagcc tggtattttc gtcgttagtg ttgtgtgatt gcatagttaa tgcaaaacgt
    36901 tacagatttg aaatccgcct taaagatata atttatgtag tttaataacc aaaaagaaaa
    36961 agaaaaagtg ctgttaatag catttaaact atttaacagc actctacaac tatttaacgt
    37021 ttataatagt aaaaaccatc cataggtcaa ttttgttcaa tttctttcca aataagagcg
    37081 ccagccccta aaattgctgc attaatttct cccatttcag aaagcaacac ttttatttta
    37141 ttccggtaga tgggcagcaa atgtttctca aggctttgtt gggtaggttt aagaatcaaa
    37201 tctcctgcct tggcaagccc tccaaaaagg aatattgctt ctgggcttgt atgtgcaacc
    37261 gaatcagcaa gtttttggcc taaaagttct ccggtatatt caaaagcttc tttggcaata
    37321 acatcacctt tagtagctgc ttctgcaatt ttatctgatg taagctcatt aaaactgatg
    37381 tcgcgcaaag ggctattctc catactttca gatagtattt caaaaactgt acgcctgata
    37441 cctgtagccg aagcataagt ttcaaggcat ccgcgacgcc cacagccaca ctggcgtcca
    37501 ttatgacgga ctatagtgtg cccaagctct ccggcaaagc catcatggcc gtatataagc
    37561 ttcccgttac ttaccaaacc acttcctaat ccggtcccaa gagttatgac aatgaaatct
    37621 ttcataaaac gggcaccgcc atacaccatc tctccaatgg ctgcagcgtt tgcatcattt
    37681 gtaagggcaa caggtacctc aaaatgagat ttgaaaagct ttgccaaagg aacaatgcct
    37741 ttccatttaa ggttagcggc atgttcaata gtgcctttgt aataattgcc attgggcaca
    37801 ccaattccaa tgcctttcaa ttgcaggtct ggctgagacg taaggttttc tttgattaat
    37861 tttgaaagtt cattaacaaa gttctctata tttcggtcag aattggtcgg gacgctgcct
    37921 tcgcaatagc agttcccttt tttatccact aagccaaact tagtattggt tccgcctatg
    37981 tcaatcccgg caacgacttc aattatattt tccattatta ttttttttaa gaggttgatt
    38041 gtttgtggtg ggcaaaatta taattttaaa atcattcgac catagttttt tactcatgta
    38101 gtatttaaat cgtgtgtgag ataaagatct aaaaatcagg ataatcagca tggttttaga
    38161 caaagctgct aaaaatcata atacttattt atcaggtttg ttttaaaagt gaagtagaaa
    38221 aacaggttgc ttcggttttg gtagcttgcc tctcttttca taatacccaa ctccaaacgc
    38281 ctgtttgtat aaggatttaa cacatacgat agagaaagtg taaagtattt caaatcatta
    38341 taagcacttt gtcccggcaa cacttttatt tccttttctc cataattttt attgccataa
    38401 gaaccccaat tatatagagc attgacaaat attcttttat gtcgataagc cgcaataata
    38461 atgttttcag aaaaattatt accccaagca tgtgctaaag actgcatgtg atgggtataa
    38521 ttcaacaagg tatcttcgtt tagccccaat ttattaccag ctttgttatg ctcatattgc
    38581 aatacaagat ttcttagggt aaaagcattg taatatttta caccaccctg ccatccggta
    38641 tttgcagcct taaacccatt atccttaata ttgctggccg ctaattgacc gtagcatgtt
    38701 aatgtattaa aaatattgac tttcatgtca aatgtcaata atgaactgct tttttcattc
    38761 tgagaggctg tatttacaaa cattacagga ttgacagctt cccaggcgaa gtctatattt
    38821 gaccttttat ttgggtaatg atatatgttg tattcaaaaa cagacaaatg aatatcttgc
    38881 gaggcaagaa aagacaatat atgcattgaa gataactttc tttcccatac accaaacgaa
    38941 gttttaggaa tagagtcgcc cataagcacc aataatgatt gggtatattt aaatttccct
    39001 actgacagag tatataatgc ataaggataa ttaaaagcat tatcggtcaa caacaacgag
    39061 cgatagccat ttccaacgaa cattttgtct gtaccaatag aaaacaaaag ctttttcgaa
    39121 acaaaccatg caatcctccc tgaagccaag ccataatcaa agccagtatc atattttctt
    39181 gcccttcctt gaccggatac agcattagta gctttaaaac gttcatacaa ataaggtaaa
    39241 aatgtagcct ggctttcata aacgtttgcc tcgaaaaaaa ggctactatt gacattgccc
    39301 caaaccctta cccctctggt gtttgttgta tagttttcat ttgtatttat ttcacggctt
    39361 aatgcaaaat caaataaggg gtcagcatat attttaactc cgacagtatc atatttgaca
    39421 atactttcat caaacaactt cttccctgca tagcgtttat gaggctttgc atcataacca
    39481 gaaaagagta ccttttcatc aatatctttt ttgcaagtgt caaaaacaaa aggcctgatg
    39541 aaggtatagc ttgtttggtt attacttacc gcaccattct cataactgag ccaataatca
    39601 ttattaaggg gcaatacagc ctggccttgc atgtcaatat tctggcagca tactcccaac
    39661 attagcaatg cccttgacat caaacgacaa aagattctat gttttactaa tttaatcatg
    39721 gcaaaacaat atccctttcg ttattaaata tggcagtact taaaccaatg ttaaaaaata
    39781 catctttgga ggaaaatgat cctttgctgt atatacccat aacagagtat aaagaaaaat
    39841 gcttgttttt atctaaccat ttaccaaacc ttagctctga ccacttcaat atttcttttt
    39901 gcccctgcca aagctttaca ttataatcat tttttctatt acgcagattg tatgacttat
    39961 aaatatcttg gcccaaatta acaatagatg tatcaagacc gacttttgct aaaacaattt
    40021 tagtcatcat ataccacgaa tgatttgaat acctgaagat gccaacacct tcggcgaagt
    40081 ttgcccccaa aggatgggca agagaaccat ataatgtccc gtaattcaat gttgttgagg
    40141 tatgcgaata ggtgtaaggt ctggccatat taatttctgt caataaagaa acttgatgtt
    40201 ctccataagc cttgctagtc ctgaaaccaa actgcattgc atatttgttg ccccaccagc
    40261 ctgagttttt gagcaattct tttaccatca aatcgtcaag gtaaagttgt ccgtacagat
    40321 aacttctttt acctaacctt aatgatgcat tgcctccaat attggcatta tccggcgaat
    40381 ggatggaata ttcaattggc ctgaaaaaga ttacagggtt caaataagcc gggtcaatcc
    40441 cgcgaactgt attggcatca cttccccacc aaataaccga ctcgtaaaac ccgatactca
    40501 acctcctggt aaggtttatg tccaaataat gaaaaacgcc atatttatct gtaaaaccat
    40561 tgtcgctaat gtcattcaaa taagcccaca tgataaaata gttcacgcgc cacgattgaa
    40621 agttgatttt cacaaatggg tatggtgccg aattctcaga cagaagtact gaacgatagc
    40681 cattacccac aaaatttcta tcgtatccgg cctgaaacaa aaaatggtta ccgggtttgt
    40741 agtttatact ccctgttatt gccgggaaaa aataaatttt ttcgctttgt gaaaaccatt
    40801 ttccgcagta tggcaatact ttaagcgtgt caaaaccatt tgtgcctttt tcaaaatatt
    40861 gcccccccgc cataatgctg gctttaatag aaaatttacc agatggttta taccttatag
    40921 aagctccttc gacaaaaagc atttgtttcc caaagtctcc agaatatctg aatccagttt
    40981 ctgtttcagg gcggattgaa atcttcccgg ttgacgttat gaaactgttt tgaactacag
    41041 aagtatcaaa tctttcatca gaataaaccc acggtttaac ggaagtgtga aaatcagaat
    41101 cgacaaacaa cgcattttca gcatataaat ataaaacaga attaacagga attgtacttc
    41161 cctgaccaga aagggcttgt atcaggataa gaacgtataa tattaaaaat acaaatcctt
    41221 tcacatgctc aatgtatatt tttcagcctt ctttgtctga taaaaaagat ggtttcaggc
    41281 ataacagaaa acaccaaagc cataataact attaacaata agaggtctat cattccccaa
    41341 tcatttaaaa ggaatacagt aatgatgata atcaggttag ccccaaggat taacaatgtt
    41401 gacttcaaat gcgtaaggcc aataaataac aacttatgat gaacgtggtt cctgtctgca
    41461 cggaatggcg aatgcccttt caatatacga ataataaaga ccctcatggt atcaaacaat
    41521 ggcactataa gtatgccaaa agcaactgat ggcgcagcat ggatataaaa tggggtatta
    41581 acatcaatat ttacttcgca aaacttaacg gcaaacactg ccataaaaag acctatcatc
    41641 agtgagccgg tatcgcccat gaaaattttg ttattccgcc caaatacatt gaaataaaag
    41701 aacgaaataa gcgaaccaaa gacggcaata gcaactatgg catattcata ttctccgcta
    41761 aggaaaaacc aagtcccaaa agctaccgaa gctaacaccc caactccaga agccaatccg
    41821 tcaataccat caataaggtt caccccgttt ataattacaa taaacacaaa caaggttagt
    41881 ataacgctaa accagtaagg aataatctga tagcccagga aaccatgtaa agaggtaaac
    41941 ctaaaatcgc ccatgataat aattatcagc gaagctatga tttgccccat cagctttttt
    42001 gtcggggcta ttatcaggat atcatctttt atccctataa aaaataaaat cactgaagca
    42061 ataacaatgt attgaaattc aggtactata ctaaatctga taaacaacag accagatatg
    42121 ataatgcctg cataaatagc aataccacct aaagtaggga caattttttt atgagctttg
    42181 cgtctgcctg gctcatcgtt aaggttctta atcctggcta cattaacaat agtgggtata
    42241 gatgtcatta ctataacaat tgcagtaata aaagctaata ccaatgataa agggtttgag
    42301 aaatacattt ctttaggttt tctgtcaaca aagttagata aaaataaata ctattggttt
    42361 atcaacccaa ttatttaatt ttttctttac ttattctcta aaaaagaagt aaaccatttt
    42421 ccagagtcct ttataatccg gctttgcgtg tcatagtcaa catacactaa cccaaaacgc
    42481 ggacgatagc cctcggccca ttcaaaatta tctgtaaatg accaggcaaa catgccatca
    42541 acctttactc cttcatactt tgcttttaac aaccagtaca aatgttcttt ataaaatttt
    42601 acacgttggg catcatttat ggcagttccc tgaactttat caggaaaagc agcaccggtt
    42661 tccgaaacca acaagctttt taccgggtat tgcgaaaact gtttcaggat tttgtacaag
    42721 ccttcgggat gcacttccca attcatttca gtaatttcgt tgcccaattt ctgaggcgaa
    42781 acccgggttg attgcagtat aggaattgcc ctgttcccgt ttactacaac cctgaaataa
    42841 tattgcagcc cccagaagtc ataatcaaag gagaggtgtt tctcatcatc tgcctgcata
    42901 tatttgtcta ttctgtgaag aatcgaagcg tcagaatatg gatatccctt ccctaaaaga
    42961 ggttcaacaa acattcggtt cataatggca tccagacgac gggcagcccg gatatcactc
    43021 tttgaattcg ggtcttgggg ctcaattggc gagcatgata ttgcagtacc aatgttggca
    43081 tttggcaaac attcacgaag tactttacct cctaaagact gggcaagggc tgcatgatgt
    43141 gccgcatgaa aaaagttgtt taaaccaatc tctccgggag catgaatacc tataccatat
    43201 ccaaataacg aaaaactgag cggttcgttt agcacaatcc agttttttac cctatcacca
    43261 aaagcgtcag cacaaacctt aacataatca caaaaagcat agacagtatc acggtttcgc
    43321 caaccacctt tgttttgcaa tgcttgaggg agatcccaat gatacaatgt tacccaaggt
    43381 tcaatgccgt tttcaataca tgcatcaata acacggttat aaaaatcaat gcctttaaca
    43441 tttatttcac ccgtaccttt tggaaaaatg cgaggccagg aaatcgaaaa cctcaacgcc
    43501 ccaaaattca ttgattttaa aagagctata tcttttttgt aatggtgata aaaatcgcag
    43561 gaggtatttg cattggtatg gtctttaatg ttgctggtat cattggcaaa ggtatcccat
    43621 acagaaatgc ctttcccgtc tgcatcccat gccccttcgc attgatatga tgcacaggca
    43681 actccccatg taaatctgcc aaaatcggca gcactcatct cttttatctc tgctttagaa
    43741 aaataagcgc ttaactctct gtgaaaaata aaaggcaatg ataatgaacc taataatttt
    43801 aaggcatcgc gacgtgatat cttttgcatc tttgtatcta taagcgtaac atgataatcc
    43861 tatggtctat cagcacaaag ataaccgtaa tcagggccga ataaaataaa taccaaccta
    43921 ctggtatata ctcatttggg ttaatgccca gtttaaagta attataggac atcagaatta
    43981 ttttctcaat gacataaaca attaccgtgg caacggcaac ccctacaatc ccatatcgag
    44041 gtatcatcag aaatgtaagg cctacgttga tgatgatatt aaaaactgca gtccacatta
    44101 ccactttgtt ctttttaagc cctatcaata tggtttgcgg aaataccaac ctgcttgtaa
    44161 tcaacaacac ataaaccatg aatacgtccg agctttgata aaaattttta gtgaaaagta
    44221 tcgggtaaat caaatcgcta aaaaacaaaa gcaatataga aatcggaaac aatatatgca
    44281 tcaaacgcaa tgatcgcttc tttaaagtaa gcaaaccaaa tttcattttc gacatagacg
    44341 aaaactgtgt aagcattgca ttgtttaatc cacttgcaag cgttacaacc aaaggcattt
    44401 cttttgcccc atacctgaaa atagcaaatt tttcgggcga aaattgagcc gaaacaatca
    44461 atccatcaat atattgagca gaaccactca ataatgaact aataatcaac ggtaaggcaa
    44521 gataaagatg ctctttcata aaagataacg acacctttat tatagcatat ttttggatta
    44581 accttaataa ccatagaaat cttatccctg aaataattac taaaccccaa atacctccct
    44641 caatacccca tcctaattta accggaataa tgacaaataa agtttgtaat gtaaacgaaa
    44701 tcagaccgta agcaagaata tgttttggtt tgttatataa taagtatatg tactcaatca
    44761 gacaagccgg gctactcaat aaaatatacc atagcaaacc atcggcataa ggaagcgcct
    44821 ggccagtttt atataccttt attccatctt taaataacca gccggctgca aaaagtaaga
    44881 gagagataaa caatatgagc aaaaaagtat taaacagctc tggcgatttc tgaaaaactt
    44941 ttccgttacg tagcttaaaa ctttgattat tattaaaaag aggtaataaa gactggatga
    45001 tacctgttat ccaaaagaat gtaacagtac ttgccaggaa caataagagt tcaaaatcgc
    45061 caatgtcttt tttggtcagg tctatacggg taaaaataac acttataatt aaaaatgaca
    45121 caaacctaag tacctgaaat acctgcaaac ccaaaatatt gcttatgacg aaccttctgg
    45181 ccaatgaaaa ttatttgaag caaaaataca ctttacaaaa caaagaacct aacttgttac
    45241 tgccaaattt agtcttattg ttgagctttt gcaatacatg ttattccaat tacgcccaat
    45301 atagtaactg tccatgccac agcaccaaac cctgtcaaat cttgcagata aacctggttg
    45361 gatggcttat acatcaggtt ttcagggtct aacatagtat tagatatact tgaaacccaa
    45421 aattcatttt gtatataaaa gggctcagac ttattatctt tcaatgaaaa agttaaaaaa
    45481 caccttaatg tcattggagt tgaatcgggc ttaaaatagt aaaatacttc agaagtctta
    45541 cctacttttg aataaacatt ctttcggacc ttattgcttt tgtcaaattg tttaataaac
    45601 tcgcttctca aataaaccgg cgttcgctga ataaatgatt taggggctac aaaagtaact
    45661 tgttcacttt ttgccattgt ccctgtgatg tctgaattgc tgtaagtaaa attcttagag
    45721 aatttgtatt cttcagaaga ggaagaagcg ttaattgtcg caatatcctt ccacaaactg
    45781 atattatgtc catttacaat tgctgaagat ttgctccaat caatgtataa aggaaaattt
    45841 gacttattat aaatttcaaa gctcattgga aaacaatttc cattaaacga atatacaact
    45901 ttaacagtat cattttcaaa aacatacgct ttgctttcat tttgattgac gtttccactc
    45961 aatgtaatat actgatactt agtacaccca gaaaaagtaa ttacgcctaa tgttattgtc
    46021 aaaatacaaa atatcctttt aatcctaatg aacctcataa cccagagttt agtgtttaaa
    46081 tgtacattaa cgaattctat gccaaattaa aacaaatttg tcttataact attcttttca
    46141 aataaaaaac agaagttgac cttctaaaaa aatatttgca gagatttatt tttccttttt
    46201 acaacaaagc cttattttag caaaaactca taccatggat gcaattaaag atcaaaaaac
    46261 acctcccatt acattgccac aggctgtatt ggcaccactt ctttctcttt ttcaagcgca
    46321 agttcatgca ggtagggcat ttctaagtta tttgcttcaa ataggttcgc cccatgttga
    46381 ggttgacgaa tctggaatga ttatagaaca agcccgtaaa gaaaaagaag cattgtataa
    46441 gcaaaccttt acttttgaaa aaaaagttaa tgaccaaaca caaaagtttg agctttcggt
    46501 ccctacttta tctcttgttc ctattgctcc gctgggaatt gactctgccg agttcgaatt
    46561 cgatttcagg gttgaaaatt ttcaggcgca tgcccaaatg caaaaatctg aaaagaaggc
    46621 tcttgaaaag gatgcctttt catctgataa tcgtccatgg tacttagttg aaaacccaat
    46681 taattttagt ggcaatgttg tgcctccttc tgaagaagct acagcgacaa gtaaaacgca
    46741 aaatgttatc aaaattaaaa ttactgttgg gaaacaaccc attccatcag gattggacaa
    46801 atttctggta tcgctcaacc aattggctga ggtaaatgaa atgaaaagtg tgcaataaaa
    46861 ttcaaaaatt gcaaatattt tgatactatt tatccgcaaa taatcatcaa gcctcaataa
    46921 aggatagtgc atacataaaa aaaaatgcac ccatatttct acgggtgcat ttacaaacta
    46981 accaacctaa aaagagattt caagaaatac tatcaacctc agtactacta acttaatgca
    47041 tatataatat gtattatacc aatcttttgt tacaaaataa atcttattta aataaaacta
    47101 actctcaata tatttcaaaa tagtagaaat tttaaccatt ttttaaatca aatatcaata
    47161 tatttgattc aaaaaatcaa ataattttat atttttagct atgtacaaca ttttcaattt
    47221 ttataattac aacaagctta gcgtataata tacgctttga aagccctcaa atactgataa
    47281 tactaatttt gacagagtta tttgacagat ttttccccct tgtttgcaac taatgaattt
    47341 agctttggcc aagcaatttt tctctcaact acaatactaa caaaattaaa caagtatgaa
    47401 acaaaatgac attgaattga aaagcaatgt aagctttaaa cgagcctttc tgtttttagc
    47461 ttcattactt attttcacgt gggccatagg ccaggaaaaa accattaccg gtcaaattgt
    47521 tgacaaaacc ggtagtacat taccaggtgt aaatattgtt gttaaaggca caacaacagg
    47581 aactgtttct gacattgatg gtaaatttag catcaaagtc cctaatgaaa acgcagtatt
    47641 gcaattttca tttattggat acgaaacaca agaagttgtt gtagggtctc aaaccagcat
    47701 tcctgttact atgaatgaag ctgttagtga gcttaatgaa gtagtagtta ttggttatgg
    47761 tacacagaaa aaaagcgacc tgacaggttc tgtagcttca gtatctggtg ctaagcttgc
    47821 cgaagtgcct gtagctaata ttgcccaagc attgcagggc cgcgcagctg gtgttagcat
    47881 tactactaat acaggtatgc ctggcggttc tgttaacatc cagattcgtg ggatgacctc
    47941 aatcaatgga acaaacccat tagttgttat tgacggtatc cccggaggtg atatgactaa
    48001 actaaacgca gcagatattg cttctgttga aattttgaaa gatgctgctt ctgctgctat
    48061 ctatggttca tcaggtggta atggtgttat ccttgtaaca acaaaaaaag gtagtgcagg
    48121 aaaaattaca accaatgtaa actattctta tggctggcaa agcattgcta acgaagtaga
    48181 aatgatgggc cttaaagatt acaacagggt attaggcgaa ctcaagttag ctcaatttac
    48241 tactaatatt gacacattag aatattacaa ctggaacgat cttttcttca aaaagaatgt
    48301 tcctatgcaa agtgctgact ttagtgttgc aggcggttct gaaaaatcaa atttctatat
    48361 tagtggttct tacctcgaac aagaaggtat tattaagaaa tcaaattaca caaaaacaaa
    48421 cttgcgtgta aaagctgacc aaaagttgac caaccatatt aagtttgatc aaaatgttag
    48481 ttattcaaac acaatcaacg aaggttacga agaatggatg tatactggtg tttaccaaac
    48541 cccggtttat aatgccatca ttatgccacc ttacatgaaa ggatatcagg acgatggaaa
    48601 atggactgtt acacctggtg gggtaagaaa cgccatggtt atggttgata acagaaatta
    48661 ctggtcaaaa aatgctgtat ttgaagccaa tgcaggttta accattgaat tgtttaaagg
    48721 attgacctat actccacgta tagccggaac aatgtcaatt cttgataatc aaaactacac
    48781 tccaaaatac tttgctaaag ctgatgatta tcaaacattt aaccttttag agaaaaatat
    48841 gcaaagaggc ttcaattgga tgttgcaaaa cgttgttact tataacacta gcatttctga
    48901 agtacacaat gtttctgtaa tggcaggtat ggaagcttca agaaactggg ggtataatat
    48961 tcaaggttcg aggcaaaata tagcaagcga tttgccaaac atgctttatt ttgttaaatc
    49021 attggacgac acttcaaatt ttcaaaatgt tacaggtaca ggttatgaaa gaaaatttgc
    49081 tgcatacttt ggacgtttgt cttatgatta taaaggatta gttcttttac agtttaacct
    49141 gcgccgagac ggtcagtcag attttgggcc taacaacagg tggggtaact tctattcagg
    49201 ttctgccggt ttcaaattca gcgaattaga agctgttaag aatttagggt taattagttt
    49261 tggtaaagta aggttgtctt atggtgaaat gggacagtat ccacgttcaa actggcctta
    49321 tttagctaaa atcttaacta ctgtccctac ttataattat tcatttaata atagggtttc
    49381 tcaaactggt gcaggccctg tacaaattgc caaccctgat attcaatggg aaaaggttca
    49441 atcacaaggt gtaggtcttg atttaggctt ttttaaagac aaagtcatgt tttctattga
    49501 ttatttcaac aaatacaaca acaacatgtt gatgtacaaa gatgttggac ttgtaaacgg
    49561 tgttttccaa accaattcag gtggtgaagg cgggcctaca agacctgaag ttaactctgg
    49621 tacagtaaaa aatagtggta tagaattatc tctctcatac aaagaaactt ttggagaact
    49681 taaatcgcag ttagacttta acttaactta tgttaccaat gagattactg atttagctac
    49741 cgactcaatt gtaaaaggaa atgttcacaa cgaattttcg ggtattacca ttagtaaaat
    49801 tggacattca attggtgaat tttggggtta tcaatcagat ggaattttta gaaaaggaga
    49861 ccctacatta accgatgcaa aagggaaacc tttcttctat actataaatg ctaaaggtga
    49921 tactgttaaa tatagcacag ctgtcactgc cggagatgca aaatgggtag atttgaacaa
    49981 tgacggtatt attgacaaca acgaccgtac ctatttagga aaccctaacc ctccattcgt
    50041 attcagcttt tctattaacc ttgcatacaa agcattagac ttttctgctt tcttcaatgg
    50101 agtatatggc aacaaaatat tcaatggtgg gaaacgttat ttctatgact ggaacaacag
    50161 aattaccaac cgtgctgctg cctttgccga tagatacaga gaacctctta cagataaaca
    50221 aggaaactta atcctcgaca accacggggt tcctattgac gaaggaaaca cttcaagcag
    50281 tatgcctaaa atgggggtta acagttgggg ccgttcttct gatttgttaa ttgaagatgg
    50341 ctcatactta aggttaaaat ctatccaact aggatataca gttccaccaa agcttaccca
    50401 aaaagtgaaa attgagaaat ttagggtgta tgtatcagta tctaatgtgt ttacattgac
    50461 aaaatactcc ggatacgatc ctgaagtggc acgttatgac tttagtgacc catcaatatt
    50521 gggtgttgat attggaggat atcctaaaac aaggatgttt acagtaggag ctaatctttc
    50581 attttaacaa ataaaatcaa ttgaatatga aaacaaaaat atataagtac ttattaatcg
    50641 gtttgttatt tagttttgta atgagctgta aagaggattt cctcaatgtt gagcctattg
    50701 caacagataa cgaagcatct ttctatttaa cccagaaaga tgcagaaatg gctactactg
    50761 ctgtatatgc ccaattagca tatatgactg cctgggacag ggaaacatct atgttattgg
    50821 gtaatatggc ttctgatgac gctgatgctg gcggtgaaga ccctggagat gttaccagtt
    50881 ttcaggatat tgcaaaactt aaacatatcc catcgaatga aaatctcagg cttccatatg
    50941 gtgtgtttta taaagctatt atgcttgcta ataaagcttt agaaaaattg cccaatatcc
    51001 ctaaaattga caaagaggca tctcctgctg caataaatca aatgatagct gaaattaaag
    51061 ctctgagggc attaaatacc tttcagttaa cacagattta tggtgaagta cctttagttg
    51121 accatgtttt aggctcttct gaatttattc agcctcgtgc taaaatgaaa gatctttatg
    51181 cttttatgga aaaagattta aaagaagcta ttgaagtact ccctttaagc tatgatgctg
    51241 aaaatgttgg aagaattaca aaaggggctg ctatggctat tttagcaaaa atttatctct
    51301 ttggttcttc ttatgcaaaa aattaccctg gggacgctcg ttttgaagga ttgaccgaaa
    51361 gatgggcaga tgctttggct atggctgaaa atgttatgaa ccttggggtt tataagcttt
    51421 taggtgctga tggcgaaaca tatagttgct ggaggggtga taaggttagc ggattccgtt
    51481 atttgtttac tactgccggc gataacagtg ctgaagcaat acttgaagta caaaatatcc
    51541 aggacggatt aaaatggttg agctccagag gttcatctat tgttcactgg tctgggcctc
    51601 gttactatta tgatgccaaa ggggtaaaac aaactacaag ctattggggc tttgatatac
    51661 ccacaaagtc actaaaagac gaatttgaag ctggtgaccc acgcttggat attactattc
    51721 acatgcctgg cggaaatgac tcaatcaact taacaggtgg ctggtataaa atttgctttg
    51781 acaattcatt aacccgtatg taccagaaca aatacgaatg ttcgtatgat gagtatcgta
    51841 aaaataccgg ttcttggaac gaagctccta tgaatttcaa aatattgcgc tatggcgaag
    51901 ttgttttgac tgctgctgaa gctgctgtaa tgcttggcaa taatgataaa gcactgaaat
    51961 atatcaatat gatacgttca cgtgcacgtg cttgtggtgg cccaggtaat actgttcctg
    52021 ctgatcttac tggtaccgta actttagatc aggtaaaaca tgaacgcagg tgcgagttag
    52081 ccttggaagg gaaacgtttc tacgacttag ttcgttgggg agatgcttct gccaagttaa
    52141 acgggtttgt taaaggaaat gatgaatctg ttgtatttgt taagggtaaa catgagtttt
    52201 tccctattcc tcaagccgag gttgatgtta gcaagggtgt tcttaaacaa tatcctggtt
    52261 gggaataaac ttgattattt tcatatagca taaaaaaagg tgcttcattt tgtgtgaagc
    52321 accttttttt atgctgatac taaaaacact atgcaaatta caatgtcgtt tattatgaca
    52381 acattttcaa tgataattgt caaggatttg ttgttaactt aaagtgttat tgaaatacaa
    52441 aggaattttt ctgttaaagg tttcgctgtt ttcattgcat cgttattgcc ttgaattttt
    52501 gtaatttttg cttcaggata aaagtttgca atgctaatac ctatgcattc ttttaatgct
    52561 tttaatgtcg aactgacgtt aatcttatga cgaattccgt tttaatttta ttgaatttat
    52621 tgtttacatc tttctcgcat ggtgtctatc caggctattt cttggtcaag tttaattttt
    52681 ttcattaatc tttccaactg attataatct tctataaata acctttgagg ctgtcttttg
    52741 aactgataaa aaagcttttt agctttttca tagtcatgat ctaaaacggc tctttgtata
    52801 tgggctattt caacataatc atagtgcatt acaaatgttt tctcagcatt tatattgata
    52861 aactcgggat aattaacaac ttgtctgaat tttcgggctt tttcgactaa aactggagct
    52921 gacgagtttc cattttcatc aatatgagta agaaacaaat ctgtataaac gctcatacct
    52981 tttgatacaa atgcaatcca atgtccatta ggcgaccatg catgccatga gttcatcagc
    53041 ggcaaattac aacttaattc tttagcagtc cccccggctg ttggtactat atacaatttg
    53101 ctgtcgggct gtaacagcat gaaattttcg gcctggcaaa atactatcca tttcccatca
    53161 ggagaaatag ctgggaaata attgctttta ccattatttg aggccccttt tattggcagg
    53221 gctgttcctc ctttgccctc gttaaacgga ataatgcaaa tgtcgtattg taaccttctt
    53281 tttcgttgta catattcgtc aataacagct tcttcagtga tgtttatttc taatgaatct
    53341 atacgttcta ctgcctttgc gcgacaaaaa ataatatttt tcccatcggg agtccagatt
    53401 gcatttgtat gaacatactt ttcgtcatta gccccgttaa gctcgcatat tttacctgtt
    53461 tgtctgtcat acactgccaa taaaccgttt acagcaaaaa aaagttgtga aaaagccaaa
    53521 ttttcgggtt caaatgggaa atttttgtgt ataacccggt cttttacagt tgtaacaata
    53581 tatcgcccat ctggagaaag ttttgaaaat aaaccgaaag tttttctatt ctcaatttga
    53641 gtccatgaca tgtaattgtt agtgttgaaa tatattgtat cattaaccgg ggctacaaaa
    53701 tatccgcctt tatctcgcaa gccggcatct aaatctaatc caatatattt ccctttatcg
    53761 ctaaacgaat gacaattgcc acatactgta aatcctttca tggctatatg aggaggttct
    53821 tttgatccaa tattgattaa ggaatagttc attgagtcaa ggttttcttc ggccaaaaca
    53881 aagggtatag gcatttgcct gtacaaaatt ggggcgccaa ccgaatcttt actaatgtca
    53941 aaaacgagcg aagcttttcc gtatttatcc agtggtttaa ttatcaattg tacttcactg
    54001 cctttacttt ccttttttat gttctcccaa acaggttctg aaagttttaa aactgttgta
    54061 ctgacaatgg tatcaactaa tagcttactg tcggatttta cagaaaaaga ccatttgccg
    54121 gtataactgc cacggtattt ccattcaaag gtaggagatg ggaactcagg aggaaaaaca
    54181 gcctgattaa acgggtattt tatgatgatt tttttgtcct gatttttttt aataataaaa
    54241 aaaataaatg ctaagaataa gcctatagcc aaaacgttta gcaatactct attttgaaag
    54301 attttcatac ttcatagttt agccgtaaag taataaaatt atcttggtac gaacaatcaa
    54361 cagaagtaaa aattagctaa taattaaata aaagttaaaa tttcatacta ttttcggcaa
    54421 ttataaaagt gcaaaagtat gtgcaaaaga ttattgtggg ttattttatg ctgtatggta
    54481 ataagctcat gcaataactc gaaaatcaaa aaccagtgca aagattttct cattgacaaa
    54541 agtaaaattt cggggatttc tgtagtttac cctcaagaaa acactttatt ccctatagat
    54601 tttcaaagcc caacgtttgt gtggaaagat tcgtttacaa acaattcaaa atggtatgcc
    54661 tgtatcagtg actcagccgg gaatattttg ttaggtgagt ttgtagaaaa taataaatgg
    54721 aaaccggatt caacccaatg ggaagaatta aagaaaaaaa atcgtgataa ttcattaaag
    54781 tttgccgtaa ttgggtttaa tgagaataat gagaatgtta cgggcaacaa actaaagttt
    54841 aaaatttcgc ccgattcagt tggtgccgag atatttttca gggcagtaac cttacctttt
    54901 tcttatgcag taaagaatgt aaataccatt gaatggtaca tgggaaatgt cagtggaggt
    54961 tcaccgcgaa aaatgcttga taatatgccg gtatgcgcca attgccatac tttttcaaaa
    55021 aatgggccaa cactggcaat ggacgttgat tatggcaatg acaagggttc ttataccatt
    55081 gaaaaagccg tagatacgtg ttttctaaat ccggaaaata taatttcgtg gacaaacttt
    55141 aaaaaagaag aaaatgaccc cacttttggc ttattgtcac aaatctctcc ttctggcgag
    55201 tatgttttgt caactgttaa agacctgtcc atatttgttg ccattgacga caacctggcc
    55261 tattcgcagc tatttttccc tgtcaaaggt attattggca tatacaatcg tacaacaaag
    55321 caatttagcg aattgcaagg cgctaatgac ccaaaattag ttcaaagcaa cccggtttgg
    55381 tcccctgata ataaaaaagt attattttcc cgcacaaact catacatcaa cgaaaggata
    55441 agaaaatccg gaagggcatt gctttatgca aaagacgtta aagagtttac tcaaggccac
    55501 aaaccattta agtttgatat ttattcagta gatttcaata atggtaaagg tgggcagccc
    55561 tcaccgctgg aaggtgcttc aaataatggt aaaagcaatt attttcctaa gttttctcct
    55621 gatggaaagt gggtagtgtt ttgccaagcc gaaaatttca tgttgttaca gcccgacagt
    55681 aaactttaca ttatggaagc caaagggggg aagccccgct tgatgaattg caatatgaga
    55741 aacatgaact catggcattc atggtcgcca aatgggcgtt ggttagtgtt ttcgtcaaaa
    55801 gaacaaagtt tatataccag gttgtacctg actcatattg atgaagaagg gaatgactcc
    55861 cctgcggttt taattgaaaa cttggtgttt gacaaacgag ctgccaatat ccccgaattt
    55921 tatcctggcg atgcaaagaa atttgtaaaa atcaaagaca acttttcaaa tactgcaggg
    55981 tattatacgc aacttgccat ggacaatata gggagcgaat actaccttag ggcttctcaa
    56041 aaccttgaac gggccatcaa cttagatgct gaatatcttg atgcttacat tcagcgaatt
    56101 ttactaaatg ccttgttaaa acaagccaat tcaaaaacag acctggcaga caagaaaaaa
    56161 gcgacagata ttattcagaa attaatagaa aaaaacggag aggacaaagc tttacttata
    56221 ctaaaagcca tgataatgtc agcaaatggc aataataaac aggctgaaga aatactcaaa
    56281 aagcttatca caaaagaacc caattattac aaggcatacg aattgttagc caatatttat
    56341 aagaaagaaa gcaaaacaga acttattatt ccgctttaca ataaaatgag ttcgcttgtg
    56401 ccggccaacg atttgcaagt gaaactgtcg ctggcgaaag cctatatctc aaccaatcaa
    56461 aactctcagg ccttagcttt gctaaatacc ctgaaaaaca aattgcctga caatgaagaa
    56521 attcgttcat tattatgcaa aatatatgtt aatacaaaaa atttgagtgc tgcgactaaa
    56581 gaaatagatt ttctgcttgc tgcagaccct gcagactatc aatacctgtt tatgcgtgca
    56641 gaaatatcgc aattacaggg gtcggtatat gaggctaata catatcagga gaaaggaaaa
    56701 atgcaacttc ttaaaaaatt ggaagccaat aaagaaaata taccattagc ttttgaacag
    56761 gcggcattgc tctctcaaga aaaaaagtac acttcagcaa tagatgttta taattctatt
    56821 ttaaatgttc tgccttcaaa ttaccaggca atgaaagaaa aagccaggat aatgctcatt
    56881 acgcagcaat ggcaggaagc tgttaagctt tatcaacaat tgttaaaaat atatgttccc
    56941 gaagaagaat tttacaacaa caaagctata gcgcaaataa acatgggcaa tcttcaagaa
    57001 gctttatcag agtttaataa tgttttgaat ttaagcccaa ccaacattga cgctttatat
    57061 aacagggcaa agctgtttaa aatgctcgga gaaccacata aggctgctaa agatatagat
    57121 aaaatcaaag aactgctaaa agcgaaaaaa aatctttcgg aaagcgaaaa aaaactttta
    57181 aatatctgat tggtttttct ttacgttgat gttgtttata caattaaaca tcatggcaac
    57241 ttctattttg ccagctggca tttattatat ttctttttta tttaatccat gaaggtgctt
    57301 ggcttttggt tgctctaaaa tattggttat ggaaatgtct tatatttatt gattttgctc
    57361 acacagtcca ctatcgaata atattttttc tttttgtctg ttatgcaaac tttcatctgg
    57421 ataaaatttt aaattgttaa ttgtcagatg gaactcctgg tttggctatt ttattactat
    57481 cgtgtttatt cacttcgttc atgagggcgc ttggcttagt tgcgttcgca agaacatggc
    57541 tgtttcattt ataggattat ttgatggcaa aaaccttcgt caacactttt ccaccattcg
    57601 tcaccaaaaa ttaactgttt gtcccaattt acccccccca tcaaatattg atagtcaatt
    57661 acttatattt tcaacagcat gaataaattt gaataacaaa tttttatcct taactttatt
    57721 aactttaaaa ctatgaaaat gaaaatcttg tttattgttg ttgcattgat tgtcatgcct
    57781 gctattagta cttattcaca aattaaagtt ttgtcatccg gggcagttaa cattggcaaa
    57841 accgggtatg aaatgattgt ctttgactac tctggttata atggtaccgg gccggtgttg
    57901 tacccatctg gcagtaacag tgctatacaa ctgggaaaag cagggaaagg cttcttgtgt
    57961 ttatatacta ctactgtata ttacccttct gatgcccggt tgaaagagaa tatcactaat
    58021 attacttctc cgttacaaac tttaaaataa ctaaaaggcg taaagtacga ttacaaagca
    58081 tcaatgtaca tgaaagatag tataaatgtt tctgctcctt ataagaagta tattgacaaa
    58141 agccggaaag acaactttgg gtttattgca caagaagtag aaaaggtatt gccacaagtg
    58201 gttaaccacg atgattctac agatctttat tccattgatt atataaagct aatcccattg
    58261 cttgttgaag ctgttaaaga ccagcaaagc cagattgact ccctcaagag tgtaataagt
    58321 aaatctaaag taaaaaccag catgaaggaa gcagttgaga ctactgaaga aacaactact
    58381 gaagtgaaca caacccaaac aaatagtatt gccaccttag ggcaaaatgc acccaacccg
    58441 tttaaccaac gaacgcagat taattattat ttgcccgaaa ctgttaaaca agctgcttta
    58501 tacatatttg atatgaatgg actgcaaata cgtacgattg ctattaatac aaaaggcaac
    58561 ggcagcatca ccctccatgc ctctgaacta aatgctggca tgtaccttta taccctcatt
    58621 gccgatggca aagaggtgga tacgaaacgg atgattttga ctaagtaaaa aggtcaaaat
    58681 ttaataatat taatcaaaag catttaagtt gtaaatagtg ctggttatca attaaataaa
    58741 gcctatttta aataattaca tcttatgaat aataaatatc atctagtgag agtaatatat
    58801 ctcttgttta ttttacaatt gattattgta tccgttaatg ctcaaaaaaa tattgaacat
    58861 gagataatta aagtatctga agcgaatttt aaaaaatatt tagagaaaat tccatatggt
    58921 catgaagatt attatgggtt tcaaaataga aatgagtttg aacaggttgt tgttggcgag
    58981 ccttatcaga tactttattt aacaaatatt gaaatgcctg aagattctat taactacgat
    59041 aaccttatta cttattctga tagttgggag gttcccctat tagtaaataa agaaaagcgt
    59101 tgttttttgc atgtgcaaaa aataataaaa gaatacaaag tagttggaat tggctataat
    59161 atggttgcta atgaattaaa tgaaacagaa acaagggcta tatttaataa taagaaggta
    59221 aataaatcta ttctttattt accttcaatt aagggagttt atttgcttag agattctctt
    59281 ggtaacaaga atagcagaga tttgaaattt ataccattag gctcaacatt aaaacatgtt
    59341 catgatataa aaaaagaaac atatagtggt gtactgatag aatattcaat gagagatttt
    59401 atgcatgtaa taaaagtaaa atataaccaa attttaaata gttagcaatg aaaacaaaat
    59461 actataaata tttagttgtt ttatttttga cagtaagcca aaatataaaa tgtcaagtgc
    59521 ttaacttgcc actattgcca gaaattcaat gtaatacttg gtgttgggca atatcttgca
    59581 ggcatatcat atattactat ggtaatgata atattacttt atgcgaaata gctgagtatg
    59641 ctagaacaag aaaccctaat agatttggac aaacaaactg ctgccaaagt attgataatg
    59701 gttgttgtaa tggtaatttt cttagtggag caggtggaat gacagaaatt ttttcgcact
    59761 gggggttaag ccctaattat acatttagtc ctttaactaa aaatgaaata actatagaga
    59821 tttcaaataa tcggcctttt gttattagat atccagggca tgttgatatt tgttatggta
    59881 ttgttgctaa tgatatttat attagggatc caggatgggg ttttgatatt agagattata
    59941 atacactcat ttctggaaca aataacaata ataaatggac tgagactatt aggataaatg
    60001 aatcaccagt tgtatgtgat tttgaaaaat cattaactgg tactatttca tatagtaata
    60061 tttataaggc ttctgggatt tatgatgtat cttgcattat taataacaat tcgattatta
    60121 caataaaagc tcctgattca ataactctta gtcctggctt ttcaattgaa ttagggtcgt
    60181 cattagaatt acaaactggc tcaaacttaa atattaattg tccttaatta aaaataaaac
    60241 tatgaaaaaa ataatacaga taattacggt tttgattgtt tttgttttga acgttcattc
    60301 gcaacaatta gttgaaaagg ataaaacttg gagtattgct caatttgatg ataagaatgg
    60361 attagatacc tgttattatt taaaaattgg ggatgatacc ttgattaacg atagaactta
    60421 ttcaaaagta ttaatatcag gagactctct aatgacaaat tggcttgaat atgacaaata
    60481 tctttgccaa gatcagaatg ttgtttattg ttatagtaaa tcggttcaaa atgaatacgt
    60541 cctttataac tttaatttat ctataggaga cagtattaag gttgaaggga atggctttta
    60601 tatttattta gattctataa agactaaaaa cacaaagaag tatttttatt tttcacgaaa
    60661 tggcgaaatt accacatggg ttgaaggtgt tggctgttta gatgatttgt ttcaaaattc
    60721 cgggaatata aatattgtag gaggatattc cactttactt tgttgcaaga ttggcaatga
    60781 tgttttgtat cataacgtaa aatacaacaa ttgtttttta acaacttcga taaaacgttc
    60841 agtagatgaa gctattgtgt ttcagaacta tcctaatcca tttaatgata agacttacat
    60901 aaatattaat aattactata ataccaaagc atttctttgc atctataatg tatcaggtgt
    60961 attatgtaaa aaaattgaag taaggcaata tggcgcaaat aatatatgta tcgagaattt
    61021 agccaaaggg gtatattttt acaattttac cattgacggg aaatataaaa gccaattaaa
    61081 aaggatgata tgctactaaa gacatttggg taaatgactt tacgattttt aagcaccagc
    61141 ttttagcaat ggccttaatt tcaaagctgt atccaagaag ataatattgg cgttcccatt
    61201 actttcaata tttgaggcac ccgcattgaa cagttgggcc agcctgtcgc aattggcctg
    61261 gttaaaaaag gcgttcatcc tttttgaaaa ttcttgttgt tcagtgtttt ggtaaagcag
    61321 ggctgtgttt tgggtaaggt tatagacaaa gctgtttcgt atgaggttga gcgacaggtt
    61381 gaaaaagctt ttttgcttct cgcggccaag tgtggccatt tgagcagacc agcccacgag
    61441 gtttatcaca tcgttttttt gcaccagttc aatgagcgat ttgaaataag cgaagttgtc
    61501 cctttcgttc tcattttccc ggatgaattc accagctttg acaaagttcc cggcagccat
    61561 cctggcgatt atttcggggt tttccacatc agggaatttt gcccggattc ctgctactaa
    61621 tgattcggca tcaatgggcg gtacatgcgt cagttggcat ctcgaaagga tagttttcag
    61681 gagcatcccc gggttttcag ataccaagat gaagtaggtt ttcagagggg gctcttccag
    61741 tattttcagt attttatttg agaccgtttc gttcatcttt tccggaagcc agataatcat
    61801 ggtctttagg tctgattcaa atggctttag gtttattttt cgcaatattt cagagctttc
    61861 gtgttcaaaa atacctgtct gcttgttttc gttgctgatt ttctcggtcc agttgttgag
    61921 gctgaagtat ccctgctcgt ggagcatttc gcgccattgg ggcagaaaat cgttactgct
    61981 ttgcttacgt gagccttcgg tgttgacagt aggaaaaaca aaatggtggt ccgggtgtat
    62041 gtatttttca aacttgaggc atgatgggca ttttccgcag gcatcagtct cggttttgtt
    62101 ggaacaatgg atgtattggg caaaagctat tgccatttgc aagcccccgt ttccttcagg
    62161 gcctgcaaac agtatagcat ggctaacacg gttttcgcgt atagcattta tcaggcgctt
    62221 cttaatgtcc gaatgcccta ttacatcagc aaatttcata tttgttgcaa aaagaagata
    62281 ttattttacc aaagaagcct tgtttacaac aacatccatc aagtagccat tgcaaacacc
    62341 ttttatttta actgttttgc caatcgatat tttagaggta tcagcaacct ggcttttccc
    62401 caatgcacaa ttgactccgg acatggctcc ttcagggcgt aaaacaacaa caacagcatt
    62461 actgtcaaca gttatttcgg ctaccgggcc ttctatttca agaatcttgc ccaaatactt
    62521 ggcatttgca gtgttttcat tgttttcaaa atcggtaacg agctgcttgc tttgtaccga
    62581 ataatctgtt ttttgttttg ccacggtatc gtctgctttt ctgaaaacaa ataaaagcac
    62641 tataaaccca attactgcaa gagccagccc aataatgagg gtatatttaa taaaagtctt
    62701 cgcttttgac ataataataa gttttaatgg ataaaggaga acaaaagtat ggtaaaaaag
    62761 aaaattgtgg cttatttttt ctcagctttc agttcgaggt taatctttac atctacaatt
    62821 ttagccaccc tgttttctac tactttaggt acttttatat cataatcggc aatagaaacc
    62881 ttgaaattgc tcgaaccaac aattacaccg tcttttaatg aaacaacaac cggcaagctc
    62941 acgttttttg tttttccatg aatggtaagc tgtccttcgg cagtaagctt agtttcggca
    63001 gaggaaagct ttgccatgtc aaagcctgcg attttgcctt tgaaatttgc cttaggataa
    63061 aggtgcgatt ccatgtaggt ttcgttgaaa tgctctgagg ctgttgccag tgtaaattca
    63121 aaagctttta taagcatagc gcaggctaca ttgccagttt gagtttccag tacgcaaaaa
    63181 acctgattgt tggtgccatc aatatcaatt acatcagtgt gcgacataaa ataagcttta
    63241 cctgttttgg taaagtactt gtcctgactg taagtagtac aaataaaaca aagtgacaaa
    63301 acaagaaata gtatgtgttt catgatatta gtgtgtaatt attgttgagt tgtttttctt
    63361 cctaaggtga aaaccataga aatgttgaag ccaagacgga tatctccatc tgtgaacgat
    63421 tctgaggatt gtgtcaacat ccccggctcg agcattgaag aactgttggt gagcatgatt
    63481 tgaaaaacgt gccctgaaac ctggatatca actccaatgt ttaccgggtc atagtatttt
    63541 tgtgtggcag tgtttctgtc gcctatcacg taaaagtatt cagcattgac cgagaaagtg
    63601 ttggtgagct tatagcgccc cccaatgcct aaagcatagt agtcattatc ttgttggggg
    63661 gtttcgacca tgttgcggtg tacaaaggtt ggcgagagct gcaacgacaa ttttggcgaa
    63721 aacatccgtg aaattagcaa ttgatgggta tagctgcacc tgcggctgaa atcatcgttt
    63781 cgttccttgt cattctcata gttataggca tcggctgcca ttgtcgaaac ccaaacaaca
    63841 tttactggga tggttctttt cgatgatttt tgttggcgaa aaaccgaaac tttggcaaaa
    63901 ccattgaaga aggcatcact ttgtctgcca atacctgcca tcaggtagtc tgtaatacca
    63961 tattcgaggc ttaggtacga gtgcgacccg tcaatgcccc aaaaacggtt gtacccttcg
    64021 cttacctggt caaaacggtg gtgaaccctg aagtcgagcc cgcctttggg tatcatttga
    64081 gtagattgaa aattgacaat gcgactggaa taaaatgtcc ctaaagcata atctacgtgg
    64141 tctgccattt gttcatccag aaaagcatca aggtcgtcct gggctttgag gccaatggtt
    64201 gagataaaag tggctaaaaa tattatataa ctgtatttca tgtgtctcat ttttttcggg
    64261 attaaaattt cttaaggttt tcacgttcat ctttggtcaa tgcccgcttg taaataagca
    64321 aatcgtcgat atatcctttg aaaaagcctt tattggcgct gcttttgcca atacaaacct
    64381 ggccgttttt atagtcaatg tttttaggga gcaggcgcca tgcatcttct ccattgattt
    64441 tgttgtaaaa tatcagtttg ttgaccccgt catttacaat ttctataata accaaattcc
    64501 attcggtata agtatcaatg gtatttgttt tatcttcagt tttttggtca cccacaatca
    64561 ggttgatgtc agaagcaccg gtagtgccat ctacctcaaa aactgttgct ccctggccta
    64621 tatcaacaag tacaggtttg ggttcactca ggtattttag ttgttgttcg ctttttagcc
    64681 aaaaacaaac cgtaagtgtg tcatattttt ggttcaatac cttaatagaa ttgttgccat
    64741 taagataaag acatgctttt gataatgcgc cacgggtatt atttgagtaa ctgacctcat
    64801 tttcagcttc aactgtctga ccattatttg ttttttcatt tagagaattg tcaaaagcaa
    64861 aaaaagcaat cagccctttg gtgccatact gatatacaga gtctgtactg gcatgttctt
    64921 ttttccaatc ctcttcattt tgataagtgc aggcagccaa catggctatt atagtaatag
    64981 cagcccatgt aaaatagttg tgcttcataa aaattagtta tagtttgagc ggtctaagtt
    65041 agggtttgaa atttaaacag acaatgatta actgattttt aacaggtttt ttaaatgttg
    65101 aaaatacact tttaaaatta aaaaaaaggg ccaatcttgt tgtatatttg ctgttgtata
    65161 aaattgactt gacaattgct taaaaattca ttatattaga ataaaaaaca atgaaacacg
    65221 aattgccagt tttgccttat gctgccgatg ctttaaatgc ggttattagt ccaaagacta
    65281 ttgaatttca ttatgggaaa caccaccagg cctatgtaaa caatctcaac aaccttatac
    65341 ccggctctgc ctttgagaat gcaagccttg aaactatcat ccgtgaggcc gatggaggta
    65401 tatttaataa tggggcccaa gtttggaacc acacctttta ttttatgact ttttctgcaa
    65461 ctccgcaggc tgaaccaacg gggcctttgg ctgatgccat agtaaaaaaa tatggttcct
    65521 ttgccgattt taaagcagct tttgcaaaat ctgcaacaac tttatttggt tctggttggg
    65581 cttggttagt actaaggccg gacaatacgc ttgatattgt ccaggaacct aatgccggaa
    65641 accctatgcg gcaaggattg aagccacttt taacctgtga tgtatgggaa cacgcctatt
    65701 atctcgatta tcaaaacagg aggccggatt atattgaggc tttttggaaa attgtagatt
    65761 ggaaaattgt ttctgaaaga tttttaaaac aataactttt gtttttgggt tattggtaca
    65821 aattttgttg acatttgcaa cttcaaaatt tgataaaatt ttgatttttc attttagtta
    65881 aaattttgaa gttcaggatt ttagaaacat ttttcacaga gtatgaatta tattgaagcg
    65941 ttcattttag cggttgttga aggtatcact gaatttttgc ccgtttcgtc aactgggcac
    66001 atggtagcct cctctacatt gatgggaata gcacatgatg actttacaaa gctttttact
    66061 attgttattc agtttggggc cattctttca gttattgtat tgtattggaa aaaatttttt
    66121 caaagtatta atttttacct gaaattattt gtgggtttta tcccggcagc tatttttggc
    66181 gtattgttta gcgaccagat tgacgcaatg ctcgaaaatg ccattattgt aggcataatg
    66241 ttaattattg gaggagtatt cttcttattt gtggacaaac tttttgaaaa aacacaagtt
    66301 gacgacaatc tgccaggcta taaaaagtcc tttatcattg gtttttttca gtgcatagct
    66361 atgataccag gggtatctcg gtcggcagcc actatcattg gggggttaag ccagaagatg
    66421 acgcgtaaag cggctgccga gttttcattt tttcttgctg tgccaaccat gtttgcggct
    66481 actaccaaga aattgtatga tttttatcag gatgggataa tgatgaatgc tgaacaggtt
    66541 aaaattcttg ctttcggaaa tattgttgct tttatagtag ccatcattgc tatcaaatct
    66601 tttatcacat ttttgacaaa gcatgggttt aagctgtttg gttattatag gattatagca
    66661 ggaatcctca ttttggtaat ggtaatgttt ggctttcaat tgaaatttta gtatgacggc
    66721 cgaagatttt aaagcaggac aagttatcct tattgataag ccgcttacct ggacttcatt
    66781 caacgtggta agcaagataa aatatgccct gaaacgtcag ttacaactga aagaccttaa
    66841 agttggccat gccgggacac ttgacccgct ggcaacaggt ttgcttattg tttgcacagg
    66901 ccgtaagact aaagagatag aatcaataca aaatactgaa aaggaatata ttgctactat
    66961 cgagtttggg aaaactaccc catcgtatga cttggaaact cagtataatg gccaattccc
    67021 ttttgaacat atagatgaag aagccataaa taaagcattt cagtctttct tgggtgaaag
    67081 tctgcaggtg ccgcctgtat tttctgccaa aatgattgac ggtaaacggg catacaaata
    67141 tgcccgcaaa ggaacagaag tagaaatgcg gcctaatatt atcaatatta gcgagttaga
    67201 aataataagt tttgaacgcc cggtattagt tgtacgcatc aaatgtagca agggtacata
    67261 catcagatcg tttgcatacg accttggcaa agccttgcaa agtggcgcac acttgaaagg
    67321 cctacgtcgc acggcctcag gaaattatca tgtaaatgat gcaatttctt tagacacatt
    67381 tcaggaaaaa ttagtaactt tgcagcctta aaattttata aaatattgaa tagcaacaag
    67441 ttgcttgttt aaacattaat attataaaca atgaaattat cccagtacaa gtttgcgttg
    67501 cctgccgagt tgattgcaag ctatccttct ccaaaccgtg atgatgcacg gttgatggta
    67561 gtcgacagaa aaacgggaga aatagagcat aaaatttttc atgaaatcat caattatttt
    67621 gaagaaaatg atgtgatggt tttcaacaat accaaagtat tcccggcacg cttgtacggg
    67681 aacaaagaaa aaacaggtgc tgagattgaa gtgtttcttt tacgcgaact taatcgcgaa
    67741 cagcgcttgt gggatgtact ggttgaccct gcccgcaaaa tacgtattgg caataaactt
    67801 tattttggtc aggacgattc gttagttgct gaagttattg acaataccac ctctcgtgga
    67861 cgtacgttgc gttttctgtt tgacggtgac tatgatgcat ttaaagaaac attgtatagt
    67921 ttaggtgaga cccctctccc aaaaacaata aaaagacctg ttgaagccga agaccgtgaa
    67981 cgttaccaga cggtatttgc acaacacgaa ggggctgttg ctgcgcctac tgcaggatta
    68041 cactttagta aacctctcct caaacgcctc gaaataaagg gcgttgagtt tggatttatt
    68101 acgttgcacg ttggcctggg cagtttccgt tctgttgacg ttgaagatct tactaaacac
    68161 aagatggatt cggagaggat tattatttca gaagaagtcg ccaatattgt taataaagca
    68221 aaagaaggta aaggaaaaat atgtgctgtc gggacaactg tgcttcgtac cctcgaaagc
    68281 tctgtaacca ccaatggtac acttaaacct tatgatggtt ggaccaacaa attcatcttt
    68341 cctccttatg attttagtgt ccccaacagt atgattacca actttcattt gcctttatca
    68401 acgcttttaa tgactgtatc ggcttttgcc gggtatgacc tgttgtttaa agcatataaa
    68461 gtggccatta aagaaaaata ccgctttggt acttacggcg atgcaatgct gattttgtaa
    68521 caaaagcaga cagtgatgga caaactgctt gcaatagttt ttctttgttg gggcatttac
    68581 tgtttagatg ctcaaacaaa gaatgacact atttgcgaca aagatatcca taccattcag
    68641 ctttatgttg aaggttggga gatgtctaac cctgttattt ttctcaactc acaagataaa
    68701 ttgcaccttt ctttcgacga cctttcggga aaccccaaaa agctttatta taccatcatt
    68761 cactgtaatg aaaagtggga acaagaaaat gtttcgtatt acgaaatagc cgaaggtttt
    68821 gaatcaaacc cgctgatgga ttataaatac tctgttagca cagtggtcaa ctatttgcac
    68881 tatgacctaa tttttcccaa cgaaaactgc agaccaattg tatctggcaa ttatttgctc
    68941 attgtttttg cagacaacga tatagagaag gttgtttttc aaagaaagtt ttatgttgtt
    69001 gaaaaatctg ctattatcga cttccgtatt atcaaacctg aaatagcacg ctatatgcaa
    69061 agacaacacc agttttatct ggttgttaca cccgatgtgc agaatagtat tgacctaagg
    69121 tcagaaatta aggctgttgt ggtgcaaaat tttaatttta acacccgaaa aacctgttat
    69181 ttggcccagt tgcaggataa taaaatattg gtgtatgaca acccggactc gaaccttttc
    69241 atgggtttaa acgagtttcg caattttgat ataaaaagta tccgttatca aagcccgcgt
    69301 atcaagtcta tctcatacct gatgaactgg tacgaagttg ttctccatcc tgactcttgg
    69361 cgcaataaaa aacaatattt ttctgaacag gatatcaatg gaaagttttt tatcgaaaac
    69421 tcaaacggaa aaacaaaaga tatagatgcc gactatgtaa tggtgtattt tcgccttcta
    69481 actcctgata gccttgaaag aggcaattta tatgtgaatg gggcattcaa taactggcaa
    69541 tgtaatgaat attcgcgttt aaaatatgat acgcttgaac atgcttataa aattaacctt
    69601 ctgctaaaac aaggttatta caattatctg tttgtataca aggaagctcc ttccagtatt
    69661 cctgattatg tttttgttga acgttcgcac tatgaaaccg aaaatgaata ccaagctttc
    69721 atctactatc aaatgccagg cgaacgctat caaaggctta taggatatgc agtagccaat
    69781 tcattgcaca aatagcactt gatttttcag atcatttagt cgaaaatagc ttctcttcta
    69841 ccaatctaat taggatatgt atcaccttga tgtgaatttc ctgcgtcctg tctgaatatt
    69901 gagtcttagg ggccctaatt tctacatctg ctattgaagc cagttttccg ccatctttgc
    69961 cagtcaaggc cagcaccttt atacccattt gttttgccac ctgggctgct tttaaaacat
    70021 tggcagagtt gccgctggtg cttattgcca ataaaacatc gcccggtttg cctattgatt
    70081 ctataaaacg tgaaaatata gcgtcaaaac catagtcatt accggtacat gttatgtgcg
    70141 atgcgtcaga tatagcaata gcgggtagtg caggacgttc gccacggaac cgccccgaca
    70201 gttcttcggc aaagtgcatg gcatcgctca tgcttcctcc attgccgcaa gagataattt
    70261 tacctccggc tttgatgctg tcgacaagca tttgactggc attgtctatg tttttgacct
    70321 gttgcgtatc ttcaataaaa cattggaggg tatgcaaggc ctctaataag tctttttcta
    70381 tgtcattggt cataagcttt tcgacagtta aaataatttc gacaaaaata cctttcgcaa
    70441 tggattgaag caaccttttt tcaaaaaaat gtcaatattg taaaaaggat cttttaaacg
    70501 gttcttttta ccaatgaata ttttttttgt tttcatcctt gtttgtgttt aacgaatcat
    70561 gctcgtttca tggcttgttt atcttctttt gcgggtgcga aaaagaagta aacaatctta
    70621 tttttgaaca tacgaaatag aattggttga agccttttga tggcattatt ctttattttt
    70681 ttcaccattg tccgatataa aactaaatat taggctaaag cccaattaca tcataacttc
    70741 gactccagcc tcaggactgg ggttgcaaat agtaacttgc tgaatgactt tagtcttggt
    70801 actattatcg gacaacagtg atacttttta tgataattaa aacaattcta aatgaaaaaa
    70861 gcatcaggtt tgtgttgttt atttatataa ttttgccaca atttattttt agacaatatg
    70921 aattttcgta ggttctttcc tcgtttccag gccgaacaat atctggtgct cttatatcgt
    70981 atttttattg tttacttgct gtattcactt agtcggttaa tcttttacct ttataatatc
    71041 gaccatttca acaatattgg gtttgatagg ctaatgatta tcttcggagg tggcttgaag
    71101 tttgatacaa ctgctatcat ttataccaac ctgttgttta tcgtgatgag tatcctgcca
    71161 ataccgtggc ggcacaacca aggttaccaa aaagtattaa aatatatatt tttcattact
    71221 aatggcattg ggttagtagc caattttgcg gatattccgt atttcaactt tgtattggag
    71281 cgtactacca tcaaagtttt tgcccagttt caacacgaaa ccaatatggt ccttttgttt
    71341 atgaggttta ttgttgatta ctggccaatt acggttttat atatcttttc tatgtttttg
    71401 atggtttacc tctacaaccg gcttgatgtt gggaaagcct ttcctaaaaa atggtacttt
    71461 tattatccat attcaatagt tgttgcctgc attgttatat tattgtctat tgcgggtatc
    71521 aggggcgacc tgaaacatag cacaaggcct attacgatga gcaatgccgg acaatatgta
    71581 aaatctcctg cagaactggc tatcgtgcta aataccccgt tctgtcttat tcgtagtacc
    71641 gagggggttg cttattcacg tcaaaactat tttgccgatg aagcatctct tgaaaaagtg
    71701 tatttaccag tgcattaccc tgataccaca aggcctaagc acccggttaa cgtggttatc
    71761 attattgttg aaagcttgaa taaagagttt atcgggagct attacccaga aatagacaat
    71821 ggccaataca aaggctatgc ccctttcctt gactcattac taaagcacgc cagatcatat
    71881 caatattcgt ttgccaatgg ccgcaaatct atagacgcat tgccttctgt attagcttct
    71941 atccctgcta tcgaatctcc ttttgtcctt tcaccatatt atagtaatac catgacaaca
    72001 ttacccaata tgctcaaaaa aatggggtac aagtctgcat tatttcacgg ggcgccaaac
    72061 ggatcaatgg gttttcttgc atttgggcaa ctggccgggg tggacgaata ttatggtatg
    72121 actgaataca ataacgacaa agattttgat ggtacatggg gcatttacga tgaagagttt
    72181 cttcaataca tggccaatac cattgataca tttagtaaac catttctggc cacagttttt
    72241 actgtttctt cgcatcatcc ctttaagctt cctgccagat atgacaagcg ttttgtgtca
    72301 aaagattttc ctttgcaacg ttgcattgaa tataccgact attctatcaa gcgttttatg
    72361 gaaacagcct caaaaatgaa atggttcaat aatacacttt tcgtaattac ggctgaccat
    72421 gttagcctta accaacgtcc tgagtttaaa aatgacattg gatattttgc agtgccgata
    72481 attttttatc atccgggaag ttcactcaat ggtatagaca aaaaaaccat agcccagcaa
    72541 atagacatat taccaacagt tgccaattat ataggttatc ctgaacctta cttctcgttt
    72601 gggcaagatt tattcaatcc tccagccaac aaatttgccg tgaacaacct ggacgatatt
    72661 tttcgatttt atcaggatgg ttatttgttt atgtttaacg ctaaagggcc ttttcattta
    72721 tacaacatga tagaagaccc gcttcttaca aaggatttgc ttggcaaaga gcctaaaatt
    72781 caggaaaaac ttgagcttac agctaaagct tttattcaac agtataaaaa ccgtatgctg
    72841 gataataaaa tcagtttaaa ataaaacttt tgtttttcaa cacttagcct cttatcccct
    72901 gaaagaagga tttttatgca gatatttact gttatttttg taataatggt aaaaaaaata
    72961 tgcggtttgg actaattctg cttgggttgc agttttgctt taatgttggc ttgtttgcgc
    73021 aagataaaaa tactcatttc tcaattttga gaaatgatat tatccgttgt atggataaaa
    73081 aacttgattc tgcatttttc tatgccgagc agctggaaaa agacgcatca aaatctaaac
    73141 gtcccgacta tgtaagcatt gctttacaat ataaaggtaa aatcaatatc cggcgtgaag
    73201 agtttagtac tgctaccggc tactttattg atgcacttag aattgaacaa gccagaaata
    73261 atgaagccca aattgctgaa ataaaattca tgcttggata tattttttat ctgatggaaa
    73321 aatacgaaaa atccaaagga tactatcaag ctgcgttgga attttatttg aaaaacaaag
    73381 atagccttgg aatggcaaaa acatattcgc acataggtaa tcttgaaact tccagggagt
    73441 attgcgagaa tcgttctata cctcaaaaag cagaggatta ttatcttggc attaattttt
    73501 ataaaaagtc attggaaata gcccataaaa tagggaataa agaaggcgag ggaatagcat
    73561 atcataacat tggcaataca tacagaaaac tcgaaaagcc tggtttggcc atttcttatc
    73621 tattaaaagc aaaacagctt tatgctgaga ttcatgacac aaacagacta atgggcttaa
    73681 agcgcccttt gagcatggtt tattcaatga ttgggcaaca tcggcaagcc atttgcgaac
    73741 taacagaatg tattgattac agcgttaaaa acaaccaatt gcagggtatt cagtttttgt
    73801 acgaagattt ggcctacata tacgaacgcg ctggtgatta taaacgttcc aaagagaatt
    73861 atgtcattta tatgaccatt cgggattcaa tttataacag cgaaaagact cataaaattt
    73921 ttgagcttga aacccgctat aatactgttc aacaagaaaa gcagataatg aaacttactg
    73981 cccaaaagaa attgcaaaga gcttatctgg gcatatctgt tgtaataata cttttattgt
    74041 tagggttagg ttactttatt tacaaagcac taaaaaataa aagagttttg gccgaaagga
    74101 acctcgaaat ccatgagcaa aggctggttc agcttgaaaa agatcataaa atattagcct
    74161 ctcaacttat cattaagggt gaagaaacag agcgtcagcg cttggcccgc gaccttcacg
    74221 acggtttggg aggtatgttg tcgggagtaa agttatcact tagcagtatg aaaggcaatg
    74281 ccattgtttc agagcaatcg gccaaagaat ttgaccatgc cctaaatatg cttgatgcat
    74341 caattaaaga acttaggagg gtggcacata acatgatgcc tgaggccctt atacgtagtg
    74401 gacttaaaga tgcgctatca gacttttgtg cttcattaaa cgaatcgtgc cagatgaaca
    74461 tcagttttca gtttgtgggt gcgtttcaac gcattaaacc tgagcttgaa atcaatagtt
    74521 ttcgcattgt acaggagcta gtcaacaatg ctcttaaaca ctcacaagcc acagagcttg
    74581 ttgttcagat gattcaggaa caagatcgct tatgccttat tgttcaggat aatggtattg
    74641 ggtttggccc tgaaatagcc aaacttcaga aaggaatggg tattgacagt gtaaagtcac
    74701 gggtagagct tatgaatggt tctattgacg tagcctcaaa gcctcgacaa ggtgttgaat
    74761 acactataga attttcttta ttataagtct aaatggctaa ctttattgac aatcacagag
    74821 ataaccccaa gtttggtatg taaaatcttg gcagcatgct gaaaaatatg tatgtttgtt
    74881 acatattttt taaaatatgt cccaaatctt aattgttgac gatcatcctg tagttgttga
    74941 aggtttacaa aagcttgtaa cagacaagaa aattgcccgg gagtgcattg tggcattttc
    75001 gtgtgccgaa tgtttaaaaa tcattgacat ctatacccct gatctgatat tacttgacta
    75061 tcaattgcct gatggcaatg gaattaattt gtgctcgcag attttaaaaa aaatgccaga
    75121 agctaaaatt cttgccatca gctctttcaa agaactaagc ctcgtaaagc agatgattgc
    75181 tggcggggca ttgggctatg ttttgaaaaa tgcctctgat gttgagatag aagaggctat
    75241 acgcaaagta ttggctggta aaaaatatct tagcgatgac attgccgaaa gcatgcagca
    75301 tgcagatagc aattttattc tcacacgtcg cgaattggaa attttacact tcatagccga
    75361 tggctttacc aatccagaga tagccgaaaa gctttttatc agccctctta ccgtagatag
    75421 tcatcgtaaa aacctgattg ttaagcttaa cgcaaaaaat accgctatgc tgatcaaagt
    75481 ggctgctcaa aaagggtttt tgtgataatt ttttaaaact atgcaaaaat gaagaaattt
    75541 ataataaaaa acatttacaa agcttttatt cttttgggta ttatcattgc agcatgtaac
    75601 aaacagcctg tcaatattgt caatcaaaag gtaaatatcc gatacgactc gttaaacttt
    75661 atttctttgg ctgatactat tgtttgcgat atggtaataa agaaccccga caaagatgac
    75721 aaatggacag aagaatgttt gcagcagctc caacgtaaaa aacttataga cactttattt
    75781 tatcaggttt ataacaacga attacaggtt ttggattatt ataccaatac tacactttgc
    75841 ccgtctgaaa taagaaaaat agaaaaggaa ttttccaata accgcgattt aattggcaaa
    75901 atccagtttc gcgaaacctg gatgtacgat gttaaaaaca aagtctttat taaaaaagtt
    75961 cattcgatga tccttggtta cgaaacattt gatgataaag ggtttgtaaa aggatataaa
    76021 cctcttttca agataatgct gccataaaga agccccgcta accaggcggg gctcataaac
    76081 caaatattaa ccaaaaaaaa ccaaaaaaac caaaaaaacc aaaaaaacca aaaaaaccaa
    76141 aaaaaccaaa aacaaaacta actactaact aacataacta attatactac aaaaataagg
    76201 ttgcaaaccc taagatttta gttaataaat gttggttttc aggtttttac atattttaac
    76261 aattctattc tgaatagttt gcattaaaaa agaaaaaccc cgcttcaaga gcggggcccc
    76321 acctaaacca attattaacc aaataaaaac ctaaacaaaa actaactact aactaactac
    76381 agcaaaatta atatctaaat atccgaaatt tttgttaata aatgttggtt tatagcattt
    76441 tacaaatatt aacaattcta tttttgtcaa atttttctat ttacctttct tcaatcttca
    76501 ggtcgccaaa taccacatct gctttaattt ctaaataagg gttattcaca tccaggctgt
    76561 cagactcgta tattgtggta ccaaacgaag ttgaattgcc atttggcagg tgtgcacccg
    76621 aaaaaacagc atcaacattt atttttacag gaaaactttt gtcaacaata accactgaac
    76681 ttccaaaaac agtggagatt ttaaccttcc cgccttctct tttagcttgt accccccgca
    76741 aatcaataac acccttactg aaaaccactg aatattcctt ccccggttca atattgtcaa
    76801 atgaagactc gccaaacact acctcagtat ctgttttgtg tcttgagaaa ggatgaaaac
    76861 tcttgccaac aattattttt atcccaatgt atataaaaat aaaagctact atcaacctga
    76921 atattggaaa atcaatatta aaaacaattt ttattatcag gcttatacca attactatca
    76981 gtaataaacc ccaaaacatc cctgaactca ttttcatttt atttaatgtt taaaattatt
    77041 ttttatcaaa agtattatcc agaatatatt gaattcaata atctttcggc gaataactta
    77101 attttaccga ccaaaaagct taatccgggc aaatgtcatt ttgtcgtttt tggggttttg
    77161 gcaaaagttt tgataaacat ataccgatag taaaccatag tttatacgtc aaaataaatg
    77221 gtattaacat attaaaatta cacatataat atgaaaaagg caaagcatga agaggtgaaa
    77281 atggaaaatg atgctcagca accaacaggc actgacaata tttcagcaca acaaaatgaa
    77341 caaatgacag aacaaaattc taataatgct gaagatgttg aaaaaactga ggcttcagtt
    77401 gctcagtttg aaaaagaagc ggccgaatgg aaagacaagt acatgcgttt atctgcagaa
    77461 tttgacaact accgtaaacg cactctcaaa gaaaaaatgg accttacaaa atatgctggc
    77521 gaagaaatgc ttaaaagcct tttgcctgta gtggatgatt ttgaaagagg ccttgctaca
    77581 ttagaacaaa gcaacgatat tgatgctgta aagcagggta tagagcttat atatgcaaag
    77641 ttcattagct ttatacagca aaaaggcctg aaagaaatcg aagctaagaa tctgccgttt
    77701 gatactgact ttcatgaagc tgttactaaa ataccggtgg aagataaaga aatgtcaggt
    77761 aaagtagtag atgttattca aaaaggatat ttgttggacg agaaggttgt gcgttttgct
    77821 aaagttgttg taggcgaata agtctgtggc catttaaaaa aattgaacat gtcgaaaaaa
    77881 gattattacg aagtacttga ggtaccaaag aatgctacca aagacgaaat taaaaaagct
    77941 tataggcagc atgccctcaa atatcatcct gaccgcaacc cgggagataa ggctgctgaa
    78001 gaaaaattca aagaagcagc agaagcatac gaggttctta gtgatgacga aaagcgtgcc
    78061 cgttatgacc gttatggcca tgctggagta ggaggcgctt caggagcagg aggctttgga
    78121 gggggcggaa tgtctatgga agacattttc tcgcactttg gtgatatatt tggagggcac
    78181 tttggaggtt ttggttttgg tagcagctca tatcaaggcc aacgcgttag caaaggccaa
    78241 aatttgcgtg taaaagttaa gcttacactt gaagaaattg caacaggcgt tgaaaagaaa
    78301 attaaagtaa ataaatacgt acaatgtagc agttgtaatg gtagtggtgc cgccaaaggt
    78361 tcatcacata ctacctgcag tacctgtcat ggacaaggcg ttgtgaccaa aattacaaac
    78421 acctttcttg gacaaatgca gtccacttct ccatgtccca gttgcggcgg cgaaggtaaa
    78481 attattactc aaaagtgtac ccgttgctat ggcgagggca ttgtaaaaga agatgagata
    78541 ataactatca aaattcctgc cggtgttgcc gaagggatgc aaatgacagt acagggcaaa
    78601 ggcaatgctg cccgtcgtgg aggtatcaat ggagatatga tagttacatt tgccgaagaa
    78661 aaacaccccg aactgttgcg cgatggtaat gacctttatt tcagtttatt tgtaacgttt
    78721 cctgttgctg cacttggagg gactgtagag gtgcctaccc tggaaggtaa agtgaaaatc
    78781 aaaatagaac ccggaaccca gccgggtaaa cttctgcgtc ttaaaggtaa aggtcttcct
    78841 gaagtaaacg gatacggcaa aggagatttg ctggtgcata tcaacgtatg gatacctaaa
    78901 aacctgacta aagacgagag aaggattata gaaaaatttg cagaatcaag caattttgat
    78961 ccaaaaccag agaaagacga gaaaaacttt tttgaaaaaa tcagaaacct gtttgaatag
    79021 actggtataa aaaagaacga ggctgtattt tatgttacaa aatgaaccat caatcaaaat
    79081 cggttactgg tttgtcacac aaaatacagc ctcgttttgt gaggtcggtg gcggattcga
    79141 accgccgttc acggttttgc agaccggtgc ctagccactc ggccaaccga ccgtttgtag
    79201 ggctgcaaag gtaaaacctt ttttaaagtt ttaaaacttt tgtcctactt ttttattttt
    79261 tatttactaa ttatctgttt ttgaatatcg taaaggccac cagattatga acatagaaac
    79321 tgccgccaac actatgaggc ttatcataaa acttggattt ttaaagaaat agaaataatc
    79381 tgtcaagaat atatcaaagc ccctaaagcc ggcaaggaat agtcccatca aagcttcccc
    79441 ggcaatcagt cccgaagcaa taagtatccc tacattttca gctattgctt tacgctcggg
    79501 cgaatattta tttctgtccg ctatcatatc tgcaatacct tttaacatgc ccccaatgaa
    79561 tatagcaaaa gtagtttgca aaggaaggta aatgcctaca caaataagca tagggctttt
    79621 ggcctgcatc aaaattaatg atgcccctaa tagcatccct gcaataatta aaggccaagc
    79681 catatcgccc ccaacaattc cttttgaaag aagagccatc aggcctgctt gcggggccga
    79741 aagatttgaa cttccaaaac cgccctggta tccgtcgcgc acaccctggg caatatctcc
    79801 gttgttcaat agcactaaga ccccaaacat aactaatgac gatagaatta ccccaacaat
    79861 atctccagtt tgcattttcc aaggtgtacc gccaaggata tgtccggctt ttaggtcttg
    79921 cagcatctct ccggctacag ctgccgaaac acaaacgata gcagcaacac ctaatacagc
    79981 tgcaacacct tcttgccctc ttactcccat caaaaccata attaatgcag ttactacaag
    80041 agctgttaat gtcaatccgc ttattggatt attgcttgac cccataatac ctaccaggta
    80101 acctgatact gctgcaaaga aaaaggccag tataagtaaa actgttgccg ccacaaatgc
    80161 tactaataat gtagtgtgaa aaataaaaaa tgtgatgata aatgtaagaa tcgctactcc
    80221 aaatatacca agaagtatcc atccaaaact tagatccttt tcatttctgg gaatatttaa
    80281 ttcgtggcct gccatggctt tttttacatc tccaacagaa cggcttactc ccgcaatgag
    80341 gctcttgcgc attttaaaca aggtgtaaaa tgccccaact aacatacccc caatagcaat
    80401 tggctttaca atatatctgg ctatttgagc tattaagcct attgttatgc tttgttcagc
    80461 ctggtcgggg ctaattccgg gattggcagc aatcatggct gtttttaaac tttccatatc
    80521 aatccccggg agcaaaaagt ataatatcat cgggattaaa ataccccatg ctaaaatacc
    80581 tccactaaag ttcaatgctg ccaaacgcgg gccaatgatg taacccacgc caagatatgc
    80641 agggctgatt tcaggagatc ctgccagaat gcctcctttg gctttaatgc ttgtgccggt
    80701 tatagtttgt tttgcaaaaa cgataaattt gtcccaatag acagcaaaaa acctcaactc
    80761 gcccaataat ttaaccaagc ctcctacgcc aatggcaata aataaaaatt ttgaatttcc
    80821 ggcagaagag cgtcctgctt tatgaatttc ggcagcagca accgattcgg ggaatggcag
    80881 ttctacatcg tccaccatga tgcgcctgag caacgctaca aacatgatcc ccaaaatccc
    80941 tcctgcaatt agtattaagc ttgctgtgat ataattgcca gggctataaa atggatccca
    81001 aagtccggct atataaaagg ccggtaaagt aaaaattgcc cctgcagcaa ccgactcgcc
    81061 tattgaccca acagtacggg caaagttttc ttccaaaatt gttccgcgaa aggcttttaa
    81121 aatagcaagg cttattacag ctgccgggta ggtagctgca attgtcatcc cggcttttaa
    81181 tcccagataa gcattggctg cccctaaaac tacgctcaac actaagccaa tgagcaatgc
    81241 cctaatggtc atttcagcca tgtttgtatt tgcaggtaca aatggcacaa atttattttc
    81301 tgacataatc taaaaatttt ctaag
//
LOCUS       DAOSPF010000005        78747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_327_length_78747_cov_7.130178, whole genome shotgun sequence.
ACCESSION   DAOSPF010000005 DAOSPF010000000
VERSION     DAOSPF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 78747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_78747_cov_7.130178"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PK252_01735"
     CDS             complement(<1..649)
                     /locus_tag="PK252_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13456.1"
                     /translation="MKNLKVYITVLIAFFCSSFLIGQKVLDKSGKRPNWVNGVVKDYI
                     IVTASGETAESAQSNALLKVKERIISAVAENVQTSSDYYRGDKTENGISTFIENFETS
                     TRTKSADIPYLKGISLSKAEEFYWEKVSENNKTRFYYHLKYPFSEMELKSLIVDFDMA
                     DKQLTLELEQIIDSSQNITSLEQISYFSLALQKMSKAFIDQRKEKADVNIARFNEM"
     gene            complement(663..1202)
                     /locus_tag="PK252_01740"
     CDS             complement(663..1202)
                     /locus_tag="PK252_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13457.1"
                     /translation="MMKNITKTLLFLVGLFVLPSLIVLAGNKPKEVKIPCADKGRSDA
                     MYFRASNSAKSNDMARAKDKALMVTKQMLASLIGSTLQAVNDNFASSSSTEFKQSMQS
                     ISREVVNQQLKSVAIACEKTDKGKDGNYECFIAVEMPKVVLVEEIVKAVDGNDKLKVD
                     FDKKKFEEIFDSEMKKLEN"
     gene            complement(1324..4056)
                     /locus_tag="PK252_01745"
     CDS             complement(1324..4056)
                     /locus_tag="PK252_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOT13458.1"
                     /translation="MKKLLTLLLVVAFISQVNAQGFLHRDKKAIVDGQGNEVILRGIG
                     LGGWMLQEPYMLNLSATNQSDMRARFEKLMGKENTDTFYTVWRANYMQKKDVDSLAAW
                     GFNSIRLPMHYNLFTLPIGKEPVEGQDTWLETGFNLVDSLLSWCETNKIYLILDMHAT
                     PGGQGKDAAISDYVDTLKSLWESDRNKAKLIALWKKLAERYASKEWIGAYDLINEPNW
                     DFENSGNKNGCNCNQNVPLLNLYKALIDTIRLVDKNHMVIVEGNCWGNRYNGMYSLAT
                     YDDNIAFSFHKYWNYNDEGSISGLLGLRNTYNVPLWCGESGENSNNWYTNAISLLEKN
                     KIGWAWWSYKKFNSYSGTNSVLQTADYDKIISYFKNGTTTLTAETAKPILMKMADNLK
                     LENCVTNYPVIDAMFRQVKSTETKPYKPNKVPGVIFFSDFDFGRNNYAYFDTDTANYQ
                     VSTNAGFTAWNSGYAYRSDGVDIQECTDATLSNGYNVGWTVAGEWMKYTVSVDSTAAY
                     RLHLRFAGQNATKMHMTVDGYDATNAIILSATGDWQKWATDSSVVVVLTKGVHAIKFF
                     TDEGGCNLNYFQLKIEGAANSVSFKPISAGTSFDGKRITLVLSHECKKPFVASLAEFV
                     VQTNKRQLSIESIGIGIAESDRVVYINLIDTIAFDEIVTVDYSGTSIINTTENTLDKF
                     EKFPVTNNLPVCVVIPAKIEAESYYFQSGLQTESCTEGGLNLGYTNAGDYMDYYIMVK
                     NAGQYPVSFRVSALNPGGIIELQIINGSNKTVLGTFNVPQTNGWQNWQTISGTVTLTQ
                     GSHLLRFYIKSPEFNINWFSIGTVTGISKMIKNEEVKLYPNPCSNILTIQSDKPLNNA
                     KFAVFDVTGRKYEVIDILQNACIATINTERFEAGNYFLMVTTTNGVVSKPFTVR"
     gene            complement(4316..7396)
                     /locus_tag="PK252_01750"
     CDS             complement(4316..7396)
                     /locus_tag="PK252_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT13459.1"
                     /translation="MTDSVLKSILRLFAILSNVKLLYQDTDKDTAFFSSRKIVNAFLA
                     QVLNPEKTARYIQIFDFHCKNIYKSRKTEPLKRISLLSTKLVLVCRQINDQLDYTKKM
                     LLLMQLLDILKDEEGAISDKLLDTVATMSEILHISADDFDKIFFFVFGSLKNKARNNS
                     FLIIYGENPVYSFRHFLHRENLQGFILVFYHENTQSYFFRHEQFNDMLTLNGRPIEPY
                     CIYILEKGYAIRSPKIQTVHFSDIAGNFLYRHSKSEIKLIARDIEFRFGNSSNGIHKF
                     SFVAQSGQLIAIMGGSGVGKSTLLNILNGNMKPQSGNVFVSDVDIYAQRDKAIDFLGF
                     VPQDDLLVEELSVFENLYYNVKLCFGNLTDEEATYKALHLLTDLNLNEIANLKVGNAL
                     NKYISGGQRKRLNIALELLREPAVLFADEPTSGLSSKDSLKVMELLKNLTYHGKIVIV
                     NIHQPSSDIFKMFDRLLVLDKGGYPVYFGNTTDSLAYFKNVIHSVNADENECLWCGTI
                     NPEEILEIIEHESLTVEGFMSGKREVLPEKWYELFLKNQTFTENDFVSKKLPQNKNGK
                     KPSVFRQFYLFLNRNIHCKLRDSQYILVTLFEAPLLAVILSFFTRFVPSNGIYTFAEN
                     LNVPAFIFMSVVVALFLGMMSSAEEIIHDARQRKRESFINLSNIAYFNSKVVYAFMLS
                     AIQMGLFVAISHWILEIHGMFFQYWFVLFSVACFANMLSLNLSSGLKSVVTIYIAIPL
                     LLIPQLLLSGTIVKYDKLHPYIRSDKYVPFIGDVMTSRWAYEALMVEQFVYNRYGDRF
                     YQFELNESNATYCNNYWIPEVLNRLDICFQLRDDSLKIAQLTRSLKLISNELVSISKE
                     LKITPFKYDSLLIPSRFDSLAFVRASNYVRHARDISVKILNNTIEKKDDYTQRLILRE
                     FSNDEGQLVKFKNRYFNQAVADEVLGKTSSDQIAVVDNCLIRKFEPVFNFPKNNFGRA
                     HYFAPAKKIGNWFLDTFYFNIIVIWLMVAICYVCLVTNVLKRFMDRISFEKSLF"
     gene            complement(7415..8176)
                     /locus_tag="PK252_01755"
     CDS             complement(7415..8176)
                     /locus_tag="PK252_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT13460.1"
                     /translation="MKTKKILGIDVGGSGIKGAVVNTKTGEMQSDRYRIETPVGAKPD
                     DVAGIIETICQKFSWEGPIGVGFPAVVQHGVAKTAANIDKSFIGLNVEKLITARTNCP
                     VKVVNDADAAGFAEMKFGAGVDKSGVVVLITVGTGIGTVIFSDGKLLPNTELGHIIMP
                     GDREAEPYACDATRKKLELSWEQWALRFNEYLHYIEDLFWPDLFIIGGGVSKHENKIF
                     SHFDVRTKIVPAQLLNNAGIIGAAIAAKQAFKDID"
     gene            complement(8257..9978)
                     /locus_tag="PK252_01760"
     CDS             complement(8257..9978)
                     /locus_tag="PK252_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOT13461.1"
                     /translation="MTCKCSFQSRFIGSIFAALLAFLLFSCSSNPQKTNSDFDKFVAE
                     YDSVVKPIYKEMNRAAWEAAISGDKIDFDRLAEWKKKYMAYHSSKDRFKILEEFKGSE
                     DITDAVKARELDVLYLMFLSNQADVDIQQQIIDKENIIEEKFNAFRAKIDGKSYSDND
                     IEDILKNSVDQKQLEAAWKAHKEIGPLVAAYIIELVKLRNKLASQLGFGNFHEMSLRI
                     SEQDPNEIERLFDELDELTGKSFIIVKNELDSILALRLKINKKDLMPWDYQNRYFQQA
                     PGIYNIKPDKYFAGKNIETIAKSFYKSIGLPVNDIIKRSHLYEKPGKNQHAFCIDIDN
                     EGDVRVLCNLKSNAMWMNTMLHELGHAVYYKNIDRDLPFILRQPAHSLTTEAIAMMFG
                     RLYSNPAWLADMKLCSKAEADEIAQQFFNNIRLEQLVFSRWVQVMYRFEKAMYENPDQ
                     DLNTLWWQLVEKYQLIRKPEGRNMPDWATKIHIAAYPCYYHNYLMGELLASQLNHYIK
                     VEILKDTIASGWPTYVNRPEVGEYLKKKVFGPGARYYWNDMIEKATGEKLSAKYYAED
                     FVVSKLK"
     gene            10174..11025
                     /gene="hypB"
                     /locus_tag="PK252_01765"
     CDS             10174..11025
                     /gene="hypB"
                     /locus_tag="PK252_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOT13462.1"
                     /translation="MCSTCGCGGAEEKGYRMYKPGEVHSHHHHEGAHHSHGHTHHHHD
                     HAHSHDHDHTHSHSHDDHEHSHPSMPSVIEIEKEILDVNDKMAERNRGYFEAKGIIAL
                     NIVSSPGSGKTSLLERTIKECSAKRKFYVIEGDQQTDNDARRIASTGAVALQINTGNA
                     CHLDAPMVHDALHELKPEENSIVFIENVGNLVCPALFDLGEQNRVVVFSVTEGEDKPL
                     KYPNMFNSANICVINKTDLLPYLDFKIELARENVKQVNPNATIIELSVKSGEGMQEWY
                     KLLNIND"
     gene            complement(11105..11458)
                     /locus_tag="PK252_01770"
     CDS             complement(11105..11458)
                     /locus_tag="PK252_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT13463.1"
                     /translation="MKENIVKDKSFKLAIRIVRLFQYLQTEKKEFIISKQLLRSGTAV
                     GAMVREAEHAESKADFVHKMAIAQKECNETLYWLELLMATQYLTEHEFMSICEETTEI
                     IKLITSIIKSAKSNS"
     gene            complement(11475..12077)
                     /locus_tag="PK252_01775"
     CDS             complement(11475..12077)
                     /locus_tag="PK252_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOT13464.1"
                     /translation="MLLHDIKNYRLILASQSPRRHFLMREAGFDFEVMVPSGVDESFP
                     DTMNPEAVPEFLAIEKAEYFKHLLTDNQIIVAADTIVLLDNKILGKPEHLDEAKDMLQ
                     KLSGRQHTVITGVCLLSNKKQVSFSDFTKVFFREFTAKEIDYYTTNYMPTDKAGAYGA
                     QEWIGYVGIEKIEGSYFNVMGLPIQKLYTELEKFLLMINC"
     gene            complement(12209..13528)
                     /locus_tag="PK252_01780"
     CDS             complement(12209..13528)
                     /locus_tag="PK252_01780"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT13465.1"
                     /translation="MNAEFEVKVLGKGTVKSPLHLSNHKGDGVFDYVNDDDRVLYNAS
                     LENFKRCKETGEEPISFEKAGPREFLFFEPAKTKVAIVTCGGLCPGLNNVIRSLVNQL
                     FYRYGVKRIVGIKYGYEGFISDYNHDVIDLTPQMVENIHLSGGTILGSSRGNQDVSKI
                     VDTLEMLNINILFTIGGDGTLRGAHAIYEEITKRNLKISVAGVPKTIDNDINLIDKSF
                     GFETASAIANEIIMNAHNEAEGAYNGIALIKLMGRDSGFIAANAALSIQEVNFVLVPE
                     MEFDLYGPHGFLKVLRKRLEERHHAMIVVAEGAGQYFFENNDQRPDASGNVKHKDIGV
                     YLKEKITEEFEAKGMNFSIKYIDPSYIIRSAAANAIDSKFCSVLAQNAVHAAMAGKTD
                     FVVGHWNNCFTLLPIPKAVEKRKKIDLESELWWNVLETTGQPVSMKD"
     gene            complement(13535..14134)
                     /gene="hisIE"
                     /locus_tag="PK252_01785"
     CDS             complement(13535..14134)
                     /gene="hisIE"
                     /locus_tag="PK252_01785"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOT13466.1"
                     /translation="MTKPDFSKLDGLIPAVIQDANTQKVLMLGFMNQEAYEKTKNEKL
                     VTFYSRTRKRLWTKGEESGNFLDVIDMLIDCDNDTLLIKVNPRGPVCHTGADTCFDEK
                     NVDAAKFLTYLQDLIDTRKKEMPDGSYTTKLFKAGINKVAQKVGEEAVELVIEAKDQN
                     DDLFLNEAADLVFHMLVLLTAKNYRIEDVIKVLEKRHKK"
     gene            complement(14127..15296)
                     /locus_tag="PK252_01790"
     CDS             complement(14127..15296)
                     /locus_tag="PK252_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOT13467.1"
                     /translation="MNFIKRIVLTLLVSVSAFSVKAAVLSDSLQVSMLTVSPGNELYS
                     VFGHTAIRVKDFKNGHDIVFNYGTFDFRTPNFYLKFALGVLDYQLSIEQFQGFLGSCM
                     AERRSVYEQKLVMPEANKQMLVSLLLDAYRPENRAYRYRFFTNNCATKVRDVIVAATG
                     EYSMFDNANRAKDSTFRMLFTPYLFNMKWERFGIELVLGKMTDEKSGYDAMFLPNNLM
                     DALSKAKVDEQLIAPVTERLYVAKGADSEGFSFTPVMAMLLIVLITLGIQFSPSKVKF
                     FDTIFFLITGFLGIFIATLSIASTHPELHFNMVALLIFPLNFFLPFVKNMKWKKNYCM
                     AIFILAIGVIALSPLLPQKFNPAVLLLGLAVAIRLFYNFVMPNSFLTKNKSKKND"
     gene            complement(15310..16068)
                     /gene="hisF"
                     /locus_tag="PK252_01795"
     CDS             complement(15310..16068)
                     /gene="hisF"
                     /locus_tag="PK252_01795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT13468.1"
                     /translation="MLAKRIIPCLDIRDGQTVKGINFENIRNVGDPVELGAEYSRQGA
                     DELVYLDITASHEKRKLFSDLVKRIAQNLNIPFTVGGGINEMSDAERLLAAGADKISI
                     NSAAVRNPQLIDQIAKGFGSQFVVVAIDAKFEDGKWTVFLNGGRIPTDKELFSWAKEA
                     QDRGCGEILFTSMNHDGVKQGFACEALSQLSLSLNIPVIASGGAGNMDHFVDVFTKGN
                     ADAGLAASIFHFKEIAIPDLKNYLHKHNIPVRLS"
     gene            complement(16180..16917)
                     /gene="hisA"
                     /locus_tag="PK252_01800"
     CDS             complement(16180..16917)
                     /gene="hisA"
                     /locus_tag="PK252_01800"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT13469.1"
                     /translation="MTNIIPAIDLIEGKCVRLSQGDYAQKKVYNEDPLEVARMFEDHG
                     ITHLHLVDLDGAKAKHVVNAKVLERIASKTDLMVDFGGGIKTNEDIELVFDCGANQAT
                     IGSIAVSNPELFVSWLDKYGPERIILGADILDDKIAISGWQEITQIELDDFLTKYQEK
                     GVQYVLCTDISKDGMLQGTSIDLYKRLKHQFREMSFIASGGITYLHEIEELSKAHIYG
                     IIIGKAFYEGRITLEDLENFIVHGSRG"
     gene            complement(16979..17566)
                     /gene="hisH"
                     /locus_tag="PK252_01805"
     CDS             complement(16979..17566)
                     /gene="hisH"
                     /locus_tag="PK252_01805"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT13470.1"
                     /translation="MHVAIIKYNAGNIMSVEFALKRLGVECEVTDDFEKIKSADKVIF
                     PGVGEAETTMRFLKNAKLDLLIKDLTQPVLGICLGLQLMCSYSEEGDTPCLGIFQEKV
                     LRFEPKLKVPHMGWNTITELKSPLLYGLSEASYVYFVHSFYAQVGEHTIAKTNYINDF
                     SAALNYKNFYATQFHPEKSSDVGLSILKNFVERVV"
     gene            complement(17578..18438)
                     /gene="purU"
                     /locus_tag="PK252_01810"
     CDS             complement(17578..18438)
                     /gene="purU"
                     /locus_tag="PK252_01810"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOT13471.1"
                     /translation="MKKQNAILLIHCEDKQGIVATVTEFLNSNNGNIVTLEQHVDHSQ
                     GMFCMRIEWELEKFLIPPDKIGDYFNTLIVPRYNMNWSLYFSGYKHKMAIFVSKMSHC
                     LFDILARYKANEWNVEIPMIISNHKELEPVCEAFQIPFYYFPITAENKREMEEKEIEL
                     LRKHDIHLVVLARYMQVLSEDFVNQFPNKIINIHHSFLPAFPGAKPYHSAYQRGVKII
                     GATSHYVTSSLDEGPIIEQDVTRVSHDDSIDDFIQKGKDLEKIVLSRAISLHLQHKLL
                     VYNNKTVVFD"
     gene            complement(18451..19578)
                     /gene="hisB"
                     /locus_tag="PK252_01815"
     CDS             complement(18451..19578)
                     /gene="hisB"
                     /locus_tag="PK252_01815"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOT13472.1"
                     /translation="MKKKALFIDRDGTMIVEPPETKQVDTLEQLEFMPKVIRSLSLIR
                     KNLDFEMVMVTNQDGLGTPLYPQENFDKVQGKMMQVFESEDIFFDAVFIDKSFPEDNK
                     PTRKPGTAMLTGYMNGSYDMSECYVIGDRMSDVMLARNLGCKAIVYGAEDKYEDIKNA
                     GLENYCALVTEDWEDIYSFLALPERIVHIRRVTSETDVNIKINLDGRGIGDVSSGLKF
                     FDHMLMQIARHSGIDMTIEVNGDLDVDEHHSIEDTGIALGEAIKKAIGNKAGMARYGF
                     CLPMDDCLAQVAIDFGGRCWLVWDAEFKREMVGDMPTEMFYHFFKSFSDAAGCNLNIK
                     AEGQNEHHKIEAIFKAFARAIRMALRRDPCNAEIPSTKGVL"
     gene            complement(19653..20699)
                     /gene="hisC"
                     /locus_tag="PK252_01820"
     CDS             complement(19653..20699)
                     /gene="hisC"
                     /locus_tag="PK252_01820"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT13473.1"
                     /translation="MINLDNLLRKNIKSLKPYSSARDEFQGEASVFLDANENPFNAPY
                     NRYPDPLQQLLKEKIAGIKNVPAGNIFLGNGSDEPIDIVIRAFCEPGENNIVAIDPTY
                     GMYKVAADINNVEYRTVKLSENFELVSTSVFAAIDKNTRLVFLCSPNNPSANYLDVSE
                     MEKIIEGFEGIVVLDEAYIDFSNHVGFLPKLSKYPNLVILQTFSKAWGLAAIRLGMAF
                     ASKEIISVFNKIKYPYNINALTQEYALKILNNDEQKNKWVKEILANRKLLVDQLSNIS
                     CIKKIFPSDANFVLVKVDDAEGLYKYLIDNKIVVRNRSKIALCAGCIRITVGSKGENE
                     QLCMALKQYSLNPG"
     gene            complement(20683..21993)
                     /gene="hisD"
                     /locus_tag="PK252_01825"
     CDS             complement(20683..21993)
                     /gene="hisD"
                     /locus_tag="PK252_01825"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT13474.1"
                     /translation="MELIKYPDRSKWKDVLKRPAIDNSLLENTVSSILADVKANGDKA
                     VLEYNAKFDGVSISSLQVTPEEIDEAVGQIDEKLKKAIILAKNNIEKFHLQQKVVEKK
                     VETTPGVTCWQRAVAIEKVGLYVPGGTAPLFSTVLMLGIPAMLAGCKEIIVCSPPMKN
                     GKLAASILYTASLIGIKKIYKVGGVQAIGAMAYGTASIPQVYKIFGPGNQYVTAAKQL
                     VNRTGTAIDMPAGPSEVAVIADATSNPAFVASDLLSQAEHGKDSQVLLFTTDENIVPA
                     IEKEVEKQLAALPRKDIATVSLGNSRVIIAPSNDEMMDIVNEYAPEHLIIATDNAMEL
                     GMKVINAGSVFLGHLTPESAGDYASGTNHTLPTNGYAKMYSGVNLDSFVKKVTYQHIS
                     DYGIFNIGPAIEKMAALEQLDGHKNAVTLRLGSVVNKYCNDKLG"
     gene            complement(22098..22955)
                     /gene="hisG"
                     /locus_tag="PK252_01830"
     CDS             complement(22098..22955)
                     /gene="hisG"
                     /locus_tag="PK252_01830"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT13475.1"
                     /translation="MEKLRIAVQKSGRLSEKTLELFNECGIGLTGGSGRLLVDSPDFP
                     VEVLYLRDDDIPQYVADGVADVGVVGENVYVETGKDVVITERLGFSKCRISLAIPKAH
                     EYKGLEYFQGKRIATTYPVILSKFLKEKNITADIHVISGSVEIAPGIGLADAIFDIVS
                     SGSTLFSNNLKEVEVVMKSEAVLISTPNLSASKQAILEDLIFRMRSVQKAKKNKYILL
                     NAPKKNLDEIIAIIPGMKSPSIVPLADEGWVSLHSVVPEKDFWNIIGKLQDKGAQGIL
                     VIPIEKMIV"
     gene            complement(23573..24178)
                     /locus_tag="PK252_01835"
     CDS             complement(23573..24178)
                     /locus_tag="PK252_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT13476.1"
                     /translation="MKKVLLLLPHGFETYEACVFIDVIGWNLVDGDQTTKLFTCGLTK
                     EVVSSFDQKFVVDYLIKEVKAEDFDALAVPGGFEEYDFYREAYSVDVQELIQQFYKQN
                     KPIASICVGALLLGQAGILKGKKATTYNQKPIRQQLLGTYGADVINEPIVVDNNIITS
                     WNPSTAIFVALLLLEMLTSKANAGYIAKIMGFDERMKVYYA"
     gene            complement(24306..25793)
                     /locus_tag="PK252_01840"
     CDS             complement(24306..25793)
                     /locus_tag="PK252_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HOT13477.1"
                     /translation="MSKFTIIDKSDNVAIALTDLNAGEMFVINNKEITLQQAIPRAHK
                     FALSVIAEGSDIIKYGYPIGKALTEIRPGQHVHAHNIKTKLEGILEYQYDQQIAENVY
                     PKEALTFKGYRRKNGDVGIRNELWIVPTVGCVNGIGEKMLQALKAEIGTSALDAIEIF
                     KHNYGCSQLGDDHENTRLILRDIVLHPNAGGVLVLGLGCENNQPSAFKELLGNYDTDR
                     IRFLETQKVGNEHEEGLKLLRELYHLAIQDKRTDVPVSELKVGLKCGGSDGMSGITAN
                     PLLGLFSDFLISQGGTTVLTEVPEMFGAETILMNRAKDKATFENIVHLINDFKAYFIQ
                     NNQPVYENPSPGNKEGGISTLEEKSLGCTQKCGKAKVSDVLKYGDRINVKGLNLLSAP
                     GNDLVSSTALGAAGCHMVLFTTGRGTPFGSFVPTTKISSNTPLFLNKPHWIDFNAGQI
                     ADGRNIDDVLKEFTAYILDIANGKLVNNEKSGYREIAIFKSGVTL"
     gene            complement(25814..27256)
                     /locus_tag="PK252_01845"
     CDS             complement(25814..27256)
                     /locus_tag="PK252_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HOT13478.1"
                     /translation="MISNNINMDKQLKTEERIIQFGEGNFLRAFVDWMIQEMNDNAGF
                     DSNVVVVQPIKNGLVDKLNSQNCLYHVVLKGIKNGKGVKDIKLNSAISRCINPYVDYQ
                     GYIKLAEIPTMRFIISNTTEAGIAFDENDKLEMQPPSSFPAKLTQLLYHRFTFYKGDI
                     SKGFIILPCELIENNANELKACIYKYCVLWSLPDAFKEWLEKSCTFCNTLVDRIVPGF
                     NKDTALELANTKNIEDNMCVDAEQFHLWVIEGPQWVKNEFPTDKANLNVLYVNDVKPY
                     RTRKVRLLNGPHTLMMPVAYLSGIEYVREAVEHPVIGKFILQAINNEIIPTLDLPAEE
                     LKSFADEVLDRFRNPFVNHALMSISLNSVSKFKSRLLPTLLDYNHKFGKLPENIVFSL
                     AALIAFYKGEYKGKAIAVKDDEPVMQFFANVWKNFSGNTDAIVHETLKNEAFWGQNLT
                     STKGLEEKVCQYLSKILKDGMLESVKELVK"
     gene            complement(27253..28281)
                     /locus_tag="PK252_01850"
     CDS             complement(27253..28281)
                     /locus_tag="PK252_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT13479.1"
                     /translation="MEKVHVSLKDLAKELKISISSVSRALKNHPDISPELAQKVQELA
                     RQKNYKPNPYAIRLLKHESKTIGIVIPDIVTYFYASVISGIESYTKEKGYSIVISSSQ
                     ESYQKEQNIIENLLNLRVDGLIVCLSQETADYKHFDKILEDNTPLVFFDRVCRTSEIS
                     SVVADNFEAARNVTRHFYENGCTRIAFINGPNHLNISKERLAGYIEGLKDCGLKISND
                     LLASCNMTPEGAKVATQKLLALKVKPDAILSINDTVAFSAMKEVKRRGYKIPDDIAFV
                     GFTDEFHASIVDPELTSVMHPTFEMGQEAARLLINQLESKTPGTPRQVVMKTTLVVRD
                     SSIKKQKK"
     gene            28476..29501
                     /locus_tag="PK252_01855"
     CDS             28476..29501
                     /locus_tag="PK252_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT13480.1"
                     /translation="MLSNPVNLPKVIFGTSALGNLYVEQPESVKTKIISECIEKSKLP
                     VVFDSAGKYGAGLALETLGKILTKLDIPAEDVLISNKLGWLRTPLLTDEPTFEPGVWK
                     NLKYDAVQRISYDGIIECFEQGNSLLGQKYTPQLVSVHDPDEYLAVADSDASYKNRFN
                     DIIEAYRALSDLKKQGKATAIGVGAKDWKVIRELYNHIQLDWVMFANSMTIMNHPIEL
                     IEFMEKLRKDGVTIINSAVFQAGFLIGGEYYDYRKIKPDTPENIRIFRWREDFHQLCR
                     KYNISPMIACVNFALRAPGVHSISLNTSKPEHVEKNVASANAIVPDDFYQDMKKAGLI
                     NDYCTFV"
     gene            29527..30753
                     /locus_tag="PK252_01860"
     CDS             29527..30753
                     /locus_tag="PK252_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOT13481.1"
                     /translation="MSTKKLTTPEYLLPFALITALFFLWGMAHGMLDVLNKHFQEILQ
                     ISKAKSGLIQFAMYMAYFSMALPAGYFMKRFGYRKGIILGLSLFAVGALMIAATTGFE
                     SYWMFLIFLFILGCGLATLETAANPYTTKLGPSESGERRINFSQSFNGLAWTVGPLIG
                     VFIYNSNSSGDGEKLKSLMLPYFVIGLVVLAVALLFVFTKLPEINEDYDEPASDSISK
                     KPLIKDRHLVLGVIAQFAYCAAQTGVFSYLINYLTDKGQPIHFDTSYGPYLLSVGFAL
                     FMIGRMSGSFMMRYFNPSRMLALYALMCVLLLPVVCANIGWFSIIALYGIFFFMSIMF
                     PTIFALAIKDLGPKTKKASSYIIMSIVGGAVFPPLMGYIADVTHSMSIGFLAPIPFFA
                     YIFYYALSGHKILTTN"
     gene            30761..33124
                     /locus_tag="PK252_01865"
     CDS             30761..33124
                     /locus_tag="PK252_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT13482.1"
                     /translation="MKQIKLFFLFLAIPLLNGRIIAQSPKQVYQPTFESLEKVNPVPE
                     WFKDAKFGIYFHWGVFSVPAYANEWYPRNMYFKGSNENKHHIETYGDITEWPYSNFIT
                     GAKDKKGNFVQFAPKLKSEGGKFDPEEWAQLFANAGAKFAGPVAEHHDGYSMWASKVN
                     PWNSKDMGPKLDLVGLFTDAIRKRNMKVFLSMHHAYNITGYFEAVPVTDDPKLQMLYG
                     QQGKEKNEAFWLAKHQEIIDFYKPDIIYQDFNLHLISQPVLLGFLAYYYNSAANQNKE
                     VVATFKDGLNKKCALLDYERGGPQDITDNYWLTDDAISSSSWSYTEGIGYYSKTALLH
                     AFIDRISKNGNMILNISPKADGTIPQEQKDVLLAFGAWLKKYGEAIYATRAWEKYGEG
                     PTKMGAAHGVFTTPVTGTTQDIRFTRSKDNTTLYAILLDWKKGQKETVIKTLSSNRIN
                     LKSLRSVQLINGEAGKYLTLQIKQDTSGLKVSLPEHSFDDMAYVLKFTFDGTIPTLDK
                     YTDLNTSPHYYIVPGNNTGALVLGADLSLSGKRKDAANQWKLEALGKGFYIIHNRNNG
                     NKVLEVKNDKSIAISDISRKDQQVWKIENTFNGLLKISNKQYAEVLLTVNIDKETVSG
                     WKVMEVCETKQEAFRNNSIPGTIEVEDFDKGCPGDAYYDRDEANAGGQYRTSEGIDIE
                     NCAAGGFNIGWTNNGEFMAYTVTVSKAATYQASFFVATPSDNTKLHLECDGVDVSGVI
                     LVPNTGGYQNWQAVQKSVKLEAGQHVLKLVVDEAPVNMDKMIFEEIK"
     gene            33133..33498
                     /locus_tag="PK252_01870"
     CDS             33133..33498
                     /locus_tag="PK252_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOT13483.1"
                     /translation="MELCHQGTFKRYCKTMELRNDAQLIEAYKKAHAKGAAWPEITQG
                     MKEVGIIDMEIYLFGTKLFMIMDTVADFDHDKAMAQLATKPRQSEWEAYMSQFQDTSA
                     EATADQKWQLMERIYKMDF"
     gene            33595..34611
                     /locus_tag="PK252_01875"
     CDS             33595..34611
                     /locus_tag="PK252_01875"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT13484.1"
                     /translation="MKAFAIKEKGVVADIEVAEPQVSTTDVLIEVNYIGLCGSDLNSY
                     RGLMPLVTLPRVPGHEISGIVIDKGKSVPTSIQIGDKATVSPYTNCGTCPACRAGRPN
                     TCEFNQTLGVQREGALTERISVPFEKVFVSKKLTLEELALIEPMSVGYHGANRGNVCE
                     TDTVLLLGCGTIGMGALVAAARKGATVIAVDIDDTKLEQAKKFGATYTINSQKQDAIA
                     VIKQLTNNEGASVVIEAAGTQATYKLALDAVCFAGRIVFIGYPKHEVSFDAPLLVRKE
                     VNIYGSRNALRVFPSVISMFEKNERPFTNLITKVFSFEQTPEAFRFWDENTGKVSKIL
                     IKVK"
     gene            34752..35594
                     /locus_tag="PK252_01880"
     CDS             34752..35594
                     /locus_tag="PK252_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT13485.1"
                     /translation="MIKLDAHQHFWLYNPDEYAWIGENMKVLQNDFLPMQLQKELDSM
                     SFDGSIVVQARQTLEETEWLLKLASEYDFIKGVVGWVDLCSDDIENQLKKYSDNPKFV
                     GVRHVVHDEPDDYFMARADFQHGISLLEKYNLTYDLLLFPKHLPLATELVRKFPNQRF
                     ILDHIAKPLIKGQVKEPWASEITKLAQWPNVYCKLSGMVTEADWQNWKAPDFAYYLET
                     VYQAFGSNRLMIGSDWPVCTVAGGYQQVMCVVTDFIKQFDDDVQKKILGMNCYRFYLS
                     KIQY"
     gene            35812..36840
                     /gene="galE"
                     /locus_tag="PK252_01885"
     CDS             35812..36840
                     /gene="galE"
                     /locus_tag="PK252_01885"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT13486.1"
                     /translation="MKKQVLVTGGTGYIGSHTAVELINEGFDVVIVDNLSNSSLDVLD
                     GIEKITGVKPDFEQFDLTDKLKTDAFFKKYNRIEAIIHFAASKAVGESVEKPTLYYRN
                     NLVSLLNLIDNMAANGIKHIVFSSSCTVYGQPEILPVTEDAPIQKAMSPYGNTKQICE
                     EILQDSIVANKALNVIALRYFNPIGAHPTALIGELPLGVPANLVPFITQTAIGLRKEL
                     SVFGNDYNTPDGSCIRDYINVVDLAQAHVVAINRMIKGLNQDAFEVFNIGTGNGISVF
                     EIINTFEKVTGVKLNYKVVGRRAGDIEKVWADTSKANNVLGWKATRTLDETLVSAWKW
                     EQYIRNKK"
     gene            36961..37863
                     /locus_tag="PK252_01890"
     CDS             36961..37863
                     /locus_tag="PK252_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT13487.1"
                     /translation="MVALLLLSLLLSGSPSHSNAVTNYEARQTIDLHGTSVEHKVLDQ
                     KGYSIHYFISGNKTGELIIFLHPAFADHRSFDNQVSFFSHEYRVIILDMLGHGLSKVG
                     KSKDKIDCTVEHIDSIMKIEGYTKAHIVGVSAGSLLAQYFALQYPQKVLSLTVLGGFN
                     INEKNREISKSQHIEKVELAFKALFSMNSFRKEVSRTNVTSLEEQKKYYEMSSLFTRK
                     SFKAMSGMGKILKNREDVKHEYPLMIMVGEKDINLAHNINNQWHEEDPDAKYCIIEGA
                     GHCANMDNPDEFNKTLLKFLKKEN"
     gene            37918..38502
                     /locus_tag="PK252_01895"
     CDS             37918..38502
                     /locus_tag="PK252_01895"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT13488.1"
                     /translation="MKIAIFLYEKVTALDFVGPYEVLSRIPNTEICFVGAEKGQIADA
                     GGLRFYTDFSLQDITQADILLIPGGFGIDALLHNTALISWIKQMDKTTLWTTSVCSGV
                     LLLAEAGIIKGKRCTTHWRRKEQLKNYDVEFVDSRYVQDGKYITSAGVSAGIDMALFL
                     TGKIIGDKGAMTIQRSIEYNPMPPYSYDSIINQS"
     gene            38514..39002
                     /locus_tag="PK252_01900"
     CDS             38514..39002
                     /locus_tag="PK252_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13489.1"
                     /translation="METSEKFEPICCPKLDPSLWEDKLLTWNDKKFVKASVFTMFYMP
                     VNFGQVMRKLDKAVSAAGATIPDWLCLSDHTSKWNMDVYLAVDKEIPGLQNVTLSGNF
                     FCKVYEGHFSDTKKWCDDFQKSTDTKGLKIDKWYMWYTTCPKCAKKYGKNYVVIVAKV
                     NS"
     gene            complement(39018..39434)
                     /locus_tag="PK252_01905"
     CDS             complement(39018..39434)
                     /locus_tag="PK252_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT13490.1"
                     /translation="MKSFRKELWFETPQRRQLINITRQIEQCLSESQVKEGLALVNAM
                     HITASVFINDDEPGLHHDFEVWLEKLAPEKPYSQYRHNGFEDNADAHLKRTVMGREVV
                     VAITNGQLDLGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(39514..>41235)
                     /locus_tag="PK252_01910"
     CDS             complement(39514..>41235)
                     /locus_tag="PK252_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT13491.1"
                     /translation="GFTYTISKDTVRFVNTSTGGEIYYWNFGDNMVTQMQNPMHKFKT
                     GTYNVSLTVYNSETECLDTYTERVTVGTVSCEAAFTFTVKPLLKQVAFTNTSKGNKYF
                     WAFDDGTYSEEPNPVKTYVNNGVYKVSLIAANADMSCKDMVTKIVTIGDSCSAAFSYF
                     ADTSKNTVSLYSNYNRPLLKHLWEMSDGSFSNEANPIITVPAKGYYTILHTVLNPVTL
                     CADKVKEVIQAGASNDDCFANFTFLPSDTGVVFINRSKGNLKGYIWNFGDNTKSTKEN
                     PTHLYKTEGLFNVCLTVINNKNITNTACKKVSSFPMRSICEANFNYIVDNETYTTQFI
                     NRSSGIPDRYYWGFGDGNSSTLLNPKNTYSKAGDYLVSLKVTSLSSTCSDEAYKLINV
                     GNPGGMRVVFITKPQNIALKAGGYPVDFIGAGLGDQAKLKWDFGDGQTDSTTTSPTHE
                     YAAPGTYNVCYTVEDPITGDSTQYCEEVIVTKGSEIETTSTMGLKVYPMPFDNQINIV
                     LEGINDGTYSFDLFDLSGRRVDNVANKISAANKIVTYNGSNLPAGSYILKASSSSGTT
                     SILIIKK"
     assembly_gap    41236..41335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41336..44157)
                     /locus_tag="PK252_01915"
     CDS             complement(<41336..44157)
                     /locus_tag="PK252_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13492.1"
                     /translation="MKKIIAVLMMLCTVFLYKMQAQSLSFDFNDAIPAIVGENSFSNT
                     GITLYKIPVPANNYSLRYYVQSGYYSSLTVYKNRSVKPFTTVKRMNTTPDGFNIITGN
                     EKDTFYFAFSNDYVNRIVWKLEVDTMYSEKGESFENPFNIYEGKNIISSGNYKAKWCK
                     YVFPENGSITVRSSSQYIYAYKAKPAYSSDFWNDNENFSDANLLNLGSFNYSASADFS
                     QLKVNGKAGDAIYIKIMDDYNTSPQWQLFLTFNKARLANGVDARSPQAINVGKGVYPL
                     LNYYESDFFSFFSFTPTISGKYQISVETSNIWSWSVVTNVEVWEPTIGEPFVFNAVAN
                     NTYVFAIGCFGDNNKVALSLENAEKTKPLSLFSNNITPFTLNKLNLTKLKDNPSVNYA
                     YKAEKNGVLQIIHPYRKAEGNLVSFYSDKRHASASEYFPLETLNDAIYVKKDDSVYLH
                     WDNPVDTFSWFAKFYNTTDFLTFKSADYNGKDADMVIDYNNHIVKATFPYDESFSSGI
                     TVDFSLVNGSKAILANETLVSGSYISFTKESDTTITVIDKVGNSQSWTLQVRKYSLAS
                     TAKIFTQFFIESVQIGIATIDNANKKITAKVEWSKNVSLTARCNISPRASSDKLSGTD
                     DLKYMYLYFDNDSDSIAKDSVRVVAEDGTENKYYIEVTRVIPTLGVSCAKPIIATAGV
                     NSGVWPINAVFAYKCVSDNAVELSGVDSKYNYLYELNGCPSTSSKYYYISPESKYAKR
                     CKKGEIIYFYFGEKLKSFTIKEIPASSEKEVISVNGMYGDYMLDKPNRTIWLNVSSNT
                     NVNMLYVKVSDGAEIWKGGSKQEYSIYVYSFPDSFEVRAMDSSKVTWYIKNYTKKEGI
                     SILSFTLPNQVGTTKIDSVNKKITCFVDKDVPLGNLIASFALSSGANAYVNGIQQYAG
                     FTKNDFNNPVYYSVRTTNDSAVWTVNV"
     gene            44271..44368
                     /gene="ffs"
                     /locus_tag="PK252_01920"
     ncRNA           44271..44368
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK252_01920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            44691..46517
                     /gene="ilvD"
                     /locus_tag="PK252_01925"
     CDS             44691..46517
                     /gene="ilvD"
                     /locus_tag="PK252_01925"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT13493.1"
                     /translation="MKNKLRSHTTTQGRLMAGARSLWRANGMTEQQMGKPVIAIVNSF
                     TQFVPGHVHLHEIGQEIKKMIEAEGFFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMVNAHKVDAILCISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGVVRDKKMD
                     LVDAMVMGADDTIADDYLDEVEKNACPTCGSCSGMFTANSMNCLNEAIGMGLAGNGTI
                     VATHKNRTKLFEKAAKRIVEITKAYYYDGDDSVLPRNIATKAAFNNAMAVDIAMGGST
                     NTVLHLLAIAKEAEVDFSMKDIDALSRKVPVLCKVAPSSHYHLEDVNRAGGILGIMAE
                     LSRANLIDTTVKRIDGLTLAQAIDKYDILSKNVTEEALDIYKSGPAGIRNLTMGSQGY
                     MYKELDKDRTQGCIRDIAHAYFPDGGLAVLYGNIAEKGCIVKTAGVDPSIFKFSGKAR
                     VFESQEDACDGILKDVQAGDVVIIRYEGPKGGPGMQEMLYPTSYIKSRHLGKECALIT
                     DGRFSGGTSGLSIGHVSPEAAAGGAIALLRDGDKIEIDIPARKINVVVSEEELKSRRA
                     QEEKRGKEAYTPAKRKRNISKALKVYASMVSSADTGAFRIIE"
     gene            46545..48254
                     /gene="ilvB"
                     /locus_tag="PK252_01930"
     CDS             46545..48254
                     /gene="ilvB"
                     /locus_tag="PK252_01930"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOT13494.1"
                     /translation="MNKEIKKITGSEAIVLSLLEEGTEIMFGYPGGAIMPVYDAMYNY
                     QDKLHHVLTRHEQGAIHAAQGYARVTGKVGVCMATSGPGATNLVTGLADAMIDSTPII
                     AITGQVGAHLLGTDAFQETDVVGISMPVTKWNIQVTKPEDIAPAIAKAFYIARTGRPG
                     PVLVDITKNAQFGMCEFSYEKCNYIRSYVPRPTIDEHKLTEAAKLINAAQKPMVLVGQ
                     GVILGNAEDDFKAFIQKSGIPFAWTILGASVMPVDHPLNVGMLGMHGNYGPNIKQNEC
                     DLLIAIGMRFDDRVTGDLSRYAKQAKVIHMDIDPSEINKNVKATVAILGNVKETLPLL
                     TRLIEKKEHTSWRAEFDACMKVEQEKVIKHDLFPEKQGLTMGEVLRRVNERTDGDAIL
                     VTDVGQHQMIGSRYFKYKNKRSFITSGGLGTMGFGLPAAMGAKMGAPDKQVILMVGDG
                     GIQMTIQELGTIKQTEIPVKIILLNNNFLGMVRQWQELFFDKRYSMTKLINPDFQKIA
                     EAYDLNHSKVTSRDLLDKAIEEMLASDKAYLLEVVVEKEDNVFPMVPTGASVSEVLLE
                     AAK"
     gene            48317..48859
                     /gene="ilvN"
                     /locus_tag="PK252_01935"
     CDS             48317..48859
                     /gene="ilvN"
                     /locus_tag="PK252_01935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT13495.1"
                     /translation="MNTVEQYTIIAFSENAIGLLNRITIIFTRRNVNIESLTVSESSL
                     HGIHKFTIVVNTTAAQVEKVVKQIEKQVEVLKAFFFTKNEIIFQEIALYKVPTEELLR
                     SNMIETIVRENNARIMEVTQDYTVIEKTGHEYETSKLFAELNKFGVKQFTRSGRIAVT
                     RSNVELLTAFLNERESSRLS"
     gene            48947..49795
                     /locus_tag="PK252_01940"
     CDS             48947..49795
                     /locus_tag="PK252_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13496.1"
                     /translation="MKISNYIDVLNIPFSVKLPNDKSLERSVQMYILVGEQLALIDTG
                     VAGSEKVIFDYIESIGRKPKEIKHILLTHTHPDHIGAVSAIKEATGAKVYVHAAEQAW
                     LEDTEKQYAERPVGGFHELVGGSCKADQVLHDGEVLHLCHNLTLKAIHSPGHSAGSVS
                     YLLQQENALFTGDAIPVKGDIPIYDDWAQSLASLEKLENLPFPAFVLPAWDSPKTGKD
                     AEEVFSHGFDMLYDVHQAFCEAKDEVGLADMQAVAASMITKLQLPAQSMNPLFIRSVM
                     SHLKVR"
     gene            complement(49815..50852)
                     /locus_tag="PK252_01945"
     CDS             complement(49815..50852)
                     /locus_tag="PK252_01945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT13497.1"
                     /translation="MNEVAENNETGELFEHHRWTVDKGQAPMRIDKFIFNRLVNASRT
                     KVQNAAEAGNILVNNKPVKSNYKVKPLDVISVVLTFPPRDTEIIPENIPLNIVYEDEH
                     LVIVDKEAGMVVHPAYGNFTGTLVNALYYKFKDLPMFQSGEMRPGLVHRIDKNTSGIL
                     VIAKTELALNKLASQFFYHTIERHYTALVWGNLTKDSGTITGNIWRSAKDRKVMAVYD
                     DPEIGKHAITHYKVIERFGMTTLVDCVLETGRTHQIRVHMKHIGHPLFSDAEYGGNTI
                     LKGPSFSKYKQFVENCFDILPRQALHARSLAFDHPATGKRVQFESPLPADMQQVIEKW
                     RGYANNNKSVE"
     gene            complement(50853..52748)
                     /locus_tag="PK252_01950"
     CDS             complement(50853..52748)
                     /locus_tag="PK252_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13498.1"
                     /translation="MANSKYIFITLITASFFFFKTEKTYGQEEFLAPVTYTVKASPVT
                     TKASLKATATQKADKQIVFFDDFWYANDSAFCYPLGTNWRDKNVCINRSFGYKPYTIG
                     VATFDGLNSKGELYKSAESDELSNADTLTSKYIDLSRYTPDDSLYFYFFYQPGGLGDM
                     PETSDSLILKFYKNSSDNGTVVWKKEGPNKDVLTNFQIVKMALKDEAFYTDSFYFRFI
                     NKVSLKNENQNPGLASNGDFWNIDYVCIIKNRTMADTAFIDNAIAQNAGSILLKYTSI
                     PWKHYRDFFYKTTKPLFNQKIYNFGSYEANVQVSLQFKEIIGNEVYPYEKNSTMIVCP
                     PHKYTDYQFETINLTSDETESTLFEIEAKIENSEADSISFNNSSKWYQRFANYYAYDD
                     GSAELGYGLKGANISAAMVALKYSTYKPDTLSAIDIYFNPTLNEENETEFNLFVWADN
                     KGKPGDSIYAERKVVEIAKGPQQFKRYILSKRILVDTVFYIGWQQKNEAFLNVGFDRN
                     DTANAFHFVNFTGIASGWAKSKVSKTGALMMRPVFGDPTIVSLKSTTKPAEFSVYPNP
                     AGNYINLTCSTNNNCKVQIYSLTGCLVLSMQSASSIYVGNLKSGMYIIKLIAPDNNVS
                     VSRFIKK"
     gene            complement(52738..53526)
                     /locus_tag="PK252_01955"
     CDS             complement(52738..53526)
                     /locus_tag="PK252_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT13499.1"
                     /translation="MGRKSVVEFLKSKPFLEQVKVMAAVFFAIVFASSIVLHCATRHN
                     KYLTVPDFSGLDLQQAEELADDYGLQIAISDSVFNIESKRGTVLDQYPRKGANVKKNR
                     RIFLIMNAINPEKVKMPDVVGVSLRQAVAILESQGLQVQSINYESDLAANLVLKQVFK
                     GKIIAPGKEIYKGSSVKLILGKSSDQSTMVPSLKGLTLQQARKKSILSYLNVNKFHFD
                     STVQNWKDSLKAVVYKQAPKDSTYSTSGNSVELWLTVKRDLNGQ"
     gene            complement(53619..54728)
                     /gene="mqnE"
                     /locus_tag="PK252_01960"
     CDS             complement(53619..54728)
                     /gene="mqnE"
                     /locus_tag="PK252_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOT13500.1"
                     /translation="MESFLHHKNKALQVIADKIYNHRRISEEEALLLYTQADTAWLGV
                     LASHAKQQASQKLVFFNRNFHLEPTNICLYQCKFCSYKRKIGEEGSWEYTYDDIARIV
                     EAYKNTSVTEIHIVGGVHPQRDLHYYGNMIKLIKSIMPAIHVKAFTAIELDFMIKQAK
                     MSLSEGFHALKEYGLDSIPGGGAEIFDAEVRKILCPEKSTGEKWLEIHREAHRAGIPS
                     NATMLYGHIEKWEHRIAHMSRIRQLQDETKGFNAFIPLKYRKENNHLSHIGEVSVVED
                     IRNYAISRLFLDNIPHIKAYWPMIGRDTTKISLSFGVDDIDGTIDDTTKIYTMAGFKE
                     KNTMTLDEMLKLIKSAGYTPTERDSVYNVIQTFEN"
     gene            complement(54812..54970)
                     /gene="rpmH"
                     /locus_tag="PK252_01965"
     CDS             complement(54812..54970)
                     /gene="rpmH"
                     /locus_tag="PK252_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT13501.1"
                     /translation="MKRTYQPSNRKRKNKHGFLERMSTANGRKVLAARRAKGRKKLTV
                     SDEHKHKA"
     gene            55197..55793
                     /locus_tag="PK252_01970"
     CDS             55197..55793
                     /locus_tag="PK252_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT13502.1"
                     /translation="MKNISLIVNIVLAVALGVLFVLHFSLRSKVNEAVSHKVVASGNS
                     NIVFVNMDSLYAKYNEYADLKEQLQKKQSKMEADINNRKIAYERKVADYQDKAQKGLL
                     LTSERQRIEQQLMGEQQELMQYGQKLQNELAEESRVLNNKLGNNIIEFLKEYNKDGRF
                     QFVLSHVYGGNLLYVNDSLDITKDVIEGLNKKYDKEKK"
     gene            55802..57103
                     /locus_tag="PK252_01975"
     CDS             55802..57103
                     /locus_tag="PK252_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13503.1"
                     /translation="MGFASGYFERYQTREPLINEAPDSNLSLVVCIPSYNENNIIRAI
                     EALYCCTPLQNKAKVIILLNYTQNNEPMKAFHERQLIETQQWISKHQRADIQFHVFLK
                     ALPAKDAGVGLARKIAMDEALRLFNLLDKRNGIIVGYDADCTCKPNYLAEIESFFIKN
                     PRAPGISVYFEHPTEGEEFEPQIYKAILQYELYLRYYIEALRYCGFPYAFQTLGSSFA
                     VRADAYASQGGMNKRKAGEDFYFLHKIIPLGRYDELNTTAVYPSPRISDRVPFGTGAA
                     VSDIISDTEGYKVFNPKSFEVLKGFFSLVPKFYKAENAFIHKYIETIGPSFAEFLKSE
                     KMVEAVANCNANSSNINVFIRRFYAWFNGLLALQCLNKLHESAFQYVPVAEASNGLLS
                     MLDKQYEQANYSENELLKIFRQRVFKFKVSWKENYSFELIE"
     gene            57110..57556
                     /locus_tag="PK252_01980"
     CDS             57110..57556
                     /locus_tag="PK252_01980"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOT13504.1"
                     /translation="MPYELFSDEYYMNEALKEAKKAFDKDEVPVGAVIVAKNHIIARA
                     HNLTETLNDVTAHAEMQVITAAASAIGGKYLTDCTLYVTVEPCFMCAGALSWAQITRV
                     VYGATDPKKGFMRSDYPLLHKKTQVTANVLRDECASLMKQFFQQKR"
     gene            57924..60080
                     /locus_tag="PK252_01985"
     CDS             57924..60080
                     /locus_tag="PK252_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT13505.1"
                     /translation="MKSYVTNQIRNIALLGSSGSGKTTLAEAMLFESGVIERKGSVEQ
                     KNTVSDYNEIEHNNKASVFSSVMYAEVNDRKLNILDNPGMDDFIGATLTSLHVADSAV
                     VVINAHNGVEVGTEIHARHLKRLNKPMILLFNQMDHEKASYEKTLEAARERLGKNITV
                     VQYPVNEGTAFDTIIDVILMKMLKYPKEGGKPEITEIPAAEAARAAEYHNALVEKAAE
                     NDDKLMELFFEKGTLEVEDLRSGLRLGVLKRDVFPVFVCSAKKSIGIGRFMEFLGNVA
                     PSPEKVEGLKTTNGNEIKVSPDGAPSLFVFKTSIEPHIGEVSYFKVITGKVTEAIDLN
                     NNNNQGKERLTQLYVCSGKNRTKVTELIAGDIGATVKLKNTKTGHTLAGGSFSEMFEK
                     TTFPEPKFRIAIKAKVEAEEEKLGEILIKLRMEDPTILIEHSKELKQTIISGQGEYHI
                     STLKWHLDHIHKIDVEFMSPKIPYRETITKVAAADYRHKKQSGGAGQFGEVHLVIEPY
                     VEGAPDPVVYKVAGKEIKLNIRDKQEVALAWGGKLVFYNCIVGGAIDARFLPAIMKGI
                     MEKMEEGPLTGSYARDIRVAVYDGKMHPVDSNEISFKLAGRNAFKEAFKNAGPKILEP
                     VYDVEVLVPSERMGDVMSDLQTRRAVVLGMSSDSGFEKISARVPLAEMDKYSTSLSSL
                     TQGRAIYNMKFAEYAPTPAEVQNELLKAYEAAEVEE"
     gene            60396..61613
                     /locus_tag="PK252_01990"
     CDS             60396..61613
                     /locus_tag="PK252_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOT13506.1"
                     /translation="MDNTTINQPVTYSLSIKGDEKSFYKELEIFTQSVIQELSIFFNK
                     EVDGLVKFLSQNKTEELRTKEEYSFELIIIGTLWNIYNGYAQKDNSFVVDTLLPHLNN
                     LRKKNQMLKPYVDDVKGYFLSRIYSNPEVDVKPEYSLYEFKRLIKWLDATGEFGEEVK
                     RMNNWYAFLLGFSDERVKQLLSKVSSYATYFSDLSRNALGQYTNGVHNYLKNIHPQMK
                     NREDYIFCGRKEEEYLMNLVGAEVLNKSLKKSFSQASKRVILLPTCMCKPDNGQCKAI
                     FAEIEGKCTSCSRNCIVNTIKNQVEDKNTTVALITHSSSFSRVLQKYENQTGVGLVGI
                     ACILNLLTGGYELKRLNIPAQCVFLNYSGCKKHWTKDGVPTTLYIEQLKQILAQKEIA
                     TTHEAKEEVKMAV"
     gene            complement(61628..62659)
                     /locus_tag="PK252_01995"
     CDS             complement(61628..62659)
                     /locus_tag="PK252_01995"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HOT13507.1"
                     /translation="MSNTSSILIIYTGGTIGMKHDEDNNSLIPIDLEALLRQMPEISK
                     LQTECKVVTFSPPIDSATMTPDIWVKLAKIIEESYNLYDGFVVLHGTDTMAYTASALS
                     FMFENLQKPIIFTGAQLPMGAVRTDGRENLITAIEIAAEKKNNLAVVPEVCIYFENKL
                     FRGNRATKYSAEHFNAFRSVNYPALAEIGIHIKYNFPSINYPTVERELKLSTKLNGKI
                     GLLKLFPGINPSFVSSVLTSPDLEAIVLETFGSGNAPTDEWFISLLKYAISNGLIVLN
                     VSQCFGGSVVMGMYRTSLALKECGVISGHDMTTEAAITKLMYLLGRYEKREDIVRGLE
                     TSLRGEITL"
     gene            complement(62720..63346)
                     /locus_tag="PK252_02000"
     CDS             complement(62720..63346)
                     /locus_tag="PK252_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT13508.1"
                     /translation="MNIAQIFRNNESWIAEKLKVDPEYFKNLSKGQNPEVLYIGCSDS
                     RVTAEELMGAKPGEVFVHRNIANMVPNTDLSVMSVITYAVSHLKVNHIVVCGHYYCTG
                     VKSAMQSADLGILNPWLRNIRDVYRLHKDELDSIADTEEKYKRLVELNVQEQCVNILK
                     TSDVQKAYKERHITVHGWVFDIHSGKLIDLQIDFDNILKGIMEIYRII"
     gene            complement(63388..63852)
                     /locus_tag="PK252_02005"
     CDS             complement(63388..63852)
                     /locus_tag="PK252_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13509.1"
                     /translation="MQIKEAQIDDLHEILDLQKLSYHENAVRYNDFHIPPLMETIEEL
                     KNDFRYSIILKAVEDSHIIGSVRGNMKNDTCFIGRLVVHPQFQNKGIGRQLMSAIENQ
                     FPAVNRYELFTGFRDDKNLYIYNKMGYKEFKQVKINDDLTFIYLEKLNTVCT"
     gene            complement(63865..64626)
                     /locus_tag="PK252_02010"
     CDS             complement(63865..64626)
                     /locus_tag="PK252_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT13510.1"
                     /translation="MELIDTHCHLFLEEFDSDVQAVVQRGVYQGVSTIINPNVDETTV
                     EALNKICSAFPDICKPAYGIHPCSVKENYKALLSIIEKEISNNKPIAIGEIGLDLYWD
                     KTFYKEQIDAFEIQLQWAKEVHLPVIIHTRDAVEQALDIVQKHPDVKGVFHAFSGTVE
                     QAKRAIDLGYVLGIGGVVTFKNGGLDKIIGSIDLKNLVLETDAPYLTPTPYRGKRNEP
                     AYLQLIVSKLSEIYQVEKEQVTEVTTLAAKSVFNL"
     gene            complement(64714..66888)
                     /gene="pnp"
                     /locus_tag="PK252_02015"
     CDS             complement(64714..66888)
                     /gene="pnp"
                     /locus_tag="PK252_02015"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT13511.1"
                     /translation="MYNLISKDIVLTDGRVITIETGKLAKQADGSVVVKMGNTMLLAT
                     VVAAKEAKEDTDFMPLSVEYKEKYAAVGRFPGGFLKREAKASDYEILVSRLIDRALRP
                     LFPDDFHAETFVTVNLISADKDTMPDALAGLAASAALAVSDIPFNGPISEVRVARING
                     QLIINPTYSQLKEADIDIMVGATAENILMVEGEMLEVSEEEMLEAIKFAHEEIKKHCK
                     VQLELTELVGKTKKREYCHEVNDDDLRKRIHSELYDKVYQVAKSGRPKHERSEAFEKI
                     KEDFLLSLSEEEQEEKKMLVERYYHEVEKEAMRNMILNEGIRLDGRKTNEIRPIWCEV
                     DCLPAAHGSSIFTRGETQSLTTVTLGTKLDEKIIDDVMIQGTDKFLLHYNFPPFATGE
                     AKASRGLSRREVGHGNLAHRALKNVIPTGAENPYTIRVVSDILESNGSSSMATVCAGT
                     LALMDAGIKIKRPVSGIAMGLITENGSGKGKYAILSDILGDEDHLGDMDFKVAGTDKG
                     ITATQMDIKVDGLPYEVMAKALQQAKEGRLHILGKITDTIAQPNADLKPHAPRIEQMT
                     IAREYIGAVIGPGGKVIQEIQRQTGATITIEEVNNSGVIDVFADNKESIDKAIRWIKG
                     IVAVPEEGEIYKGKVKTLTAFGAFVEILPGKDGLLHVSEIDWKRIENVADVLKEGDEI
                     EVKLIEIDKKTNKLRLSRKVLLPKPEKPEVKKTERKNDVSQQ"
     gene            complement(66942..67211)
                     /gene="rpsO"
                     /locus_tag="PK252_02020"
     CDS             complement(66942..67211)
                     /gene="rpsO"
                     /locus_tag="PK252_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT13512.1"
                     /translation="MYLTSEKKKEVFAEFGGAATNTGGAESQIALFTTRINHLTEHLK
                     ANHKDYSTQRALLKLVGARRRQLDYLKEKDIERYRSILKALNIRK"
     gene            67364..71494
                     /locus_tag="PK252_02025"
     CDS             67364..71494
                     /locus_tag="PK252_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT13513.1"
                     /translation="MRKVFLFLLFISLIKQGFSTEKILFSKLDINAGLSQNHINCIFK
                     DSKGFVWIGTMSGLNRYDGSLFKIFRNDANDSASIIDNFIISITEDNFGCLWILTRLG
                     YNIYNPVDESFSRQAPECFSAIGSDFTQINEMTKDIDGGLWFAQSNLGVIYYNPLENK
                     CELLKVNPKNTECLSSKAVRAITNDTEGNLLTINDNAVIERIDHKTKKVTQRITSIRN
                     ICGDLAFYKIMCDKENLIWVYSTNVASGIYQINPRTLKTRIFNTKSLPALNNNIIRAV
                     EDDGLGRIWIGTDHGGINIIDKKADKITVLTSDNNNEYSLSQNAITCIYKDNTNIVWV
                     GTYKRGVNFYHPNLVKFDLYRHESSKANSIGFDDINCFAEDKKENLWIGTNNGGIFYF
                     DRKTNTFKNYKHNPSDPASLPNNVILKLYIDKDDKLWVASYYGGLSYFDGQKFVRFKH
                     DDKNPQSLSDDKVWDIFEDSQGNFWIGTLGSGLDLFDRKKNIFYHYKYGEVNSVSSNY
                     ILHIAEDKNRNLWLATALGVNMLEHKTGRFVYFNNIPSDKNSLCNNNVTFVFPDSRGL
                     IWIGTREGLSIYNPFTRKINSYFMNDGLPSNTIQSITEDNLHHIWISTTNGLTRFFIK
                     SFTNNKLEFDIFNYDKSDGLQGSEFNEKACLKTSKGELVFGGANGFNIFKPQQIKSNT
                     TPPPVYITDLYLFNEKVKVNKPVFKRIVLTKSLMNNKKLKLRYSENIFSIEFAALNYL
                     HPEKNRFKYKLEGFNETWIETNSLHPKATYTNLDPGRYIFRVIASNDDGVWNKEGASL
                     EIVVSPPFWRSVIAFVLYFLIIFGILIYARHQVVKKARIKFSLENERKEAKRLHELDM
                     LKIRFFTNISHEFRTPLTLIITPLEKLLKTTQDTILLGQLNLIHRNAKRLLNLVNQLL
                     DIRRLEVEPFKLNLQLGDLIYFVRDITTSFSDFGEKKNIQLIFKSNVEYINIAFDADK
                     MEKILFNLLSNAFKFTYENGKIEVNIEYNKLGDNSKDAVTIKVKDTGIGIPHELQESI
                     FEPFIQNTEMPANIINQGSGIGLALTKEFVRLHNGRIWVESEPGKGSCFYVELPVNVS
                     AEAVGGNNIETVLINAEKEALPENETDEPSNKTKSSLMLVEDNEDLRFYLKDNLRIHY
                     QIFESGNGTDALKQIMEVVPDLVVSDIAIPGIDGIELCKRLKNDKRTSHIPVILLTAH
                     LDNTQKVNGFEAGADDYLTKPFSFEILESRIKNLISLRDSLRKTFKTNFDLNPSEVEI
                     VSLDEKFLKKTLEVVEKQIGNPDFSVEDLSKEMGMSRVHLYKKLLFLTGKTPSEFIRL
                     IRLKRAAQLLQKSQLTVAEISYQTGFNNPKYFARYFRDEFGILPSAYAHEYKNKQE"
     gene            complement(71504..72919)
                     /locus_tag="PK252_02030"
     CDS             complement(71504..72919)
                     /locus_tag="PK252_02030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT13514.1"
                     /translation="MTNPRKAKALYIATDFFMAVISWTCFFVFRKYVIEQFDFSQVVS
                     QMLHDSKFYLGITLIPLCWVFLYYLSGYYNFIYNKNKSTDAVSSFQAVFIGTVVIFFF
                     FLLDDQVRNYKNYYITFITLFAIQYLLTLAGRWIITASKQRKVRNGQLSFDTIIVGCQ
                     QQAKNIFDELTLKKAIYGNRFIGYIQTSDNNSKFNELPCLGTIQDFPDIIKEYSPSEI
                     IIATESSESEKTKEILNWLGYSEITVKIVAEISSHLKGQLKISNILGTALFEIDHQLM
                     PLWQQALKACIDFTGAVLAILLFLPLIIIIAIGVKVTSKGPIFFRQERIGKNGKPFVL
                     YKFRSMYVDAEKNGPNLSTQNDRRCTPFGRLLRQTKLDEIPNFYNVLKGDMSLVGPRP
                     ERKYYIEQIVKIAPNYKQLQKIKPGITSLGQVRYGYASNIEQMVKRLRYDLIYLENMS
                     LRTDFLVIYYTIVIIFKGRHI"
     gene            complement(72959..74410)
                     /locus_tag="PK252_02035"
     CDS             complement(72959..74410)
                     /locus_tag="PK252_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT13515.1"
                     /translation="MMSPYFILCIILVYFVGIYIVSHVTARKADNSTFFIGNRKSPWF
                     IVAFGMIGASISGVSFISVPGWVGTSQFSYMQIVLGYLAGYAFIALVLMPLYYKLELT
                     SIYTYLQKRFGETSYISGAMFFLISRTIGSAFRLFLSVSVLQYVVFDHMGVPFWLTAS
                     CALLFVYYYTRIGGVRTIIYTDTIQTAFMLFSLIGVVVILARFLHLDFAGVFNAIRDS
                     NYSKVFFWTEYSDKRFFFRQFLSGAFIAITMTGLDQDLMQKNLSCKNIREAQKNMFWF
                     SLSLIPVNILFLVVGAMLYIFAAQTGFVIPANSDMLFPAVATLDVMPVFFVIIFTIGL
                     ISATFASSDSAITALTTSFTLDILKANQKSDEYLAKTRKRVHLAISLLIIGLIMLFHV
                     VNNQNVISAVFTAAGYTYGPLLGLFSFGLFTKYKLKEKLVPYVTIASPVVCLFLNFLF
                     PDFFSFEILIINGMLTFMGLFVIRKKQIPENAN"
     gene            74488..75114
                     /gene="recR"
                     /locus_tag="PK252_02040"
     CDS             74488..75114
                     /gene="recR"
                     /locus_tag="PK252_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT13516.1"
                     /translation="MNSPTFSSQLLEEAVEQFAKLPGIGRKTALRMALFLLKQPIEDV
                     NLFSASISRLRNEIKFCMICSNLSDADVCPICNDKQRDNSLVCVVENAKDVLSIENTG
                     QYHGVYHVLGGIISPMEGIGPADLRIEQLEQRVAEGTIKEIIFAFSATAEGDTTSFYL
                     YRRLSRFSVQISSIARGVAIGDELDYTDTVTLGRSIQNRLPFEVKQNK"
     gene            75121..76056
                     /locus_tag="PK252_02045"
     CDS             75121..76056
                     /locus_tag="PK252_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13517.1"
                     /translation="MYKLKFIVALAELFVLAFIAKSQTNPELANKIRANVVIGIGNEA
                     LPSFFDPIPSGDENIIYTVGVSDPYANDSVALMQAKARAVLLASVLNGCRFSGVFDNY
                     NNSGNTSKLQELYRIKSAVPLIGSLTIVDSLQTRFNEKILLIKFDKTGKDTLTKEVLI
                     ELFKTQRKLDYGTQIDYNIKVEERYNNVDFPYIYTNVDGSFEILSKLDNNKSKVPPGI
                     YQYEQNSSDSTKNVVYLQHKGLWCGILQAFTNELALQASTSKSVNRQVGQNSGDSYMG
                     YLSREILNNAFTSRIIKIFINLDQLQISLTKIQKL"
     gene            76092..76661
                     /locus_tag="PK252_02050"
     CDS             76092..76661
                     /locus_tag="PK252_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13518.1"
                     /translation="MKKILYLTLVSAALVLLNTSCGGPKKMSATEDSQEVILPLSGKE
                     FQSDKEFYRAKASGKSPDIATAKKIALNNAKAEIAGLISSKIKTVTDNYTNQRSVADA
                     QDFESKFETMTREVVNQQLVDVAIIGEKLIKKGSTYDYWIGIEVSKKAILEGINNSIS
                     KNQKLAIDYDKKKFEEIYNAEMEKMEAQQ"
     gene            complement(77283..78512)
                     /locus_tag="PK252_02055"
     CDS             complement(77283..78512)
                     /locus_tag="PK252_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOT13519.1"
                     /translation="MTFFYHIFLALFYFGTCVASLFDEKIRKFLIGRKGIFGTLKKAD
                     LKNKNVIWFHAASLGEFEQGRPVIEEIKRRDKNVTILLTFFSPSGYEIRKNYAGADVV
                     VYLPFDFTWMARKFVRLANPKAVFFIKYEFWHNYLNQLHQKNIPTYCFSSIFRPQQVF
                     FKWYGGWYRSMLKYFNHIFVQNQQSKELLASIGITNTSICSDTRFDRVYELASQAKQL
                     PVIEKFINGHKAFIAGSSWPADEKLIIQYFNAYPNPVKLIFAPHEIKEDGINSIVSSI
                     NKKVIRYSQAEKVNLSEYDVLIIDNVGMLSSVYRYGYAAYIGGGFGKGIHNILEAATF
                     GIPVVFGPNYKKFREAVELAEKGGAFPIANINELTKILDKIHTDKVFLEKAGTISRDY
                     VADGRGATGLILDKTGF"
BASE COUNT        24676 a        15555 c        14525 g        23891 t
ORIGIN      
        1 gcatttcgtt gaacctggca atattcacat cagccttttc ttttctctgg tctataaaag
       61 cttttgacat tttttgtaag gccaatgaga aatatgagat ttgctcaaga gaagtaatat
      121 tttgcgagct gtcaataatc tgctcaagct caagcgttag ctgtttgtca gccatgtcaa
      181 agtccacaat gaggctttta agctccattt cgctaaaagg atatttcaaa tgatagtaaa
      241 aacgggtttt attattttca cttacctttt cccagtaaaa ttcctctgcc ttacttaaag
      301 atataccttt caaatatggg atatcagcag actttgtgcg ggttgatgtt tcaaaatttt
      361 cgataaatgt tgaaatcccg ttctctgttt tgtcgccacg atagtaatcg ctgctggttt
      421 gaacattttc ggccacagca gagatgattc gttcttttac tttcagcaaa gcattggatt
      481 gggcagattc agctgtttcg cctgatgccg taacgatgat ataatctttc acaacaccat
      541 ttacccaatt gggccgtttg ccacttttat caagcacttt ctggccgatc aaaaatgagc
      601 tgcagaaaaa tgctattaac actgttatat aaacttttaa attcttcatg acctttattt
      661 tattaatttt ctaatttttt catttcacta tcaaatattt cctcaaattt ctttttatca
      721 aaatcgactt tgagcttatc attgccatcc acagctttta caatttcttc taccaataca
      781 acctttggca tctcaactgc gataaagcac tcataattgc catccttgcc cttgtcggtt
      841 ttttcacagg caatagcaac gcttttcaat tgctggttga caacttcacg agagatagac
      901 tgcatagatt gcttaaactc agtacttgag ctactggcaa aattatcgtt tacagcttgc
      961 aaagtactac caataagcga tgcaagcatc tgctttgtaa ccatcaaagc cttatctttg
     1021 gccctggcca tatcgttact tttagctgag tttgatgccc tgaaatacat agcatcactt
     1081 cttcccttgt ctgcacaagg aattttaact tctttgggtt tatttcctgc taatacaatt
     1141 aaagacggta aaacaaacaa accaacgaga aacaataagg tttttgtaat gtttttcatc
     1201 attttttgat ttaaagttta gtataaacaa atgtaataaa aataacttta ggttgacaga
     1261 aaaaagaaag ccctgccaga aaagctcctg acagggcagt ataattaagt tggttgtttg
     1321 tttttaccta acagtaaaag gctttgacac aaccccgttt gtggttgtta ccattaaaaa
     1381 gtaattgcca gcctcaaaac gctcagtatt aatagtagca atacaagcat tttgcaaaat
     1441 atcgataacc tcgtatttac ggccagttac atcaaacaca gcaaatttag cattattcaa
     1501 aggcttatca ctttgaatag tcaaaatgtt ggaacatgga ttggggtata atttaacttc
     1561 ttcattttta atcattttac taataccagt aaccgttcca atgctaaacc aattgatatt
     1621 gaactcagga cttttaatgt aaaaccttaa caaatggctc ccctgagtta aagtaacagt
     1681 acctgaaata gtctgccaat tttgccatcc attggtttga gggacattaa aagttcccaa
     1741 cacggtttta tttgagccat tgattatttg taattcaatt attccaccag gattcagagc
     1801 agaaacccga aaagaaaccg ggtattgtcc ggcatttttg accataatat aataatccat
     1861 ataatctccg gcattagtgt agcccaaatt tagcccacct tcagtacagc tctcagtctg
     1921 aaggccactt tgaaaataat agctttcagc ctcaattttt gcaggaataa caacacaaac
     1981 aggtaaattg ttggtcacag gaaatttttc aaatttatct aatgtatttt cagtagtgtt
     2041 tattattgaa gtgccagaat aatcaacagt tacaatctcg tcaaaagcaa ttgtatcaat
     2101 taaattgata taaaccacac ggtcagactc agcaatacct atacctatac tttcaataga
     2161 aagttgacgt ttattggttt gtacaacaaa ttctgctaaa gaagccacaa aaggcttttt
     2221 acactcgtga cttaatacaa gggttatacg cttgccatca aatgaagtac ctgctgatat
     2281 tggtttaaaa gaaaccgaat tagctgcacc ttcaattttt aactggaaat aattaaggtt
     2341 acaccctcct tcatctgtaa aaaactttat agcatgcact ccttttgtaa gcacaacaac
     2401 tacagagcta tctgtagccc acttttgcca gtcaccagta gctgaaagga taattgcatt
     2461 agttgcatca taaccatcaa cagtcatgtg cattttggtg gcattctggc cagcaaaacg
     2521 aagatgaaga cggtatgcag cagtactatc gacagacaca gtatatttca tccattcgcc
     2581 agctacagtc catcctacat tataaccatt agagagtgtt gcatctgtac attcctgaat
     2641 atccacccca tcactgcgat aagcatagcc cgaattccag gcagtaaagc cggcattggt
     2701 actaacctgg taattagcag tatctgtatc aaaataggca taattgttgc gaccgaagtc
     2761 aaaatcggag aaaaatatta cacctggcac cttgtttggc ttataaggtt tagtttcggt
     2821 agatttaacc tgtctaaaca tagcatcaat aacaggatag ttggttacac aattttccaa
     2881 cttaagatta tcagccatct tcatcaagat tggttttgct gtttcggcag tcaatgtcgt
     2941 agtcccattt ttaaaatagc taataatctt atcataatca gctgtttgca atactgagtt
     3001 tgttcctgag taagaattaa actttttgta cgaccaccag gcccagccta ttttattttt
     3061 ctcaagtaaa gagatagcat tggtgtacca gttgttagag ttttcgccag attcgccaca
     3121 ccaaagaggt acattgtaag tatttctgag gcccagcaat ccacttatag aaccttcatc
     3181 attgtaattc cagtatttat gaaagctaaa ggcaatatta tcatcatacg tagccaaaga
     3241 atacatgcca ttatatcgat tgccccaaca gttgccttct acaataacca tgtggttttt
     3301 atcaacaagc ctgatagtat caataagtgc tttgtacaaa ttcagcaaag ggacattttg
     3361 attgcagttg caaccgtttt tatttccact gttctcaaaa tcccagttag gctcgttgat
     3421 aaggtcgtaa gccccaatcc actctttaga agcatagcgc tctgccagtt ttttccacaa
     3481 agcaataagt tttgccttat tccgatcgct ctcccacaat gattttaagg tgtccacata
     3541 atcagatata gcagcatctt tgccttgtcc tccgggagtg gcatgcatgt cgaggattaa
     3601 ataaatttta tttgtttcgc accaggaaag aaggctatct acaagattaa aacccgtttc
     3661 aagccatgta tcctggcctt caaccggttc tttccctata ggcaaagtaa aaaggttgta
     3721 gtgcatcggc aacctgatac tattgaagcc ccatgctgca agcgaatcga catccttttt
     3781 ctgcatataa tttgcacgcc aaacggtata aaaagtatca gtattctcct ttcccattaa
     3841 cttttcaaaa cgggcacgca tatcgctttg attggtagca ctaaggttca acatataagg
     3901 ttcctgaagc atccatccgc caaggccaat acctcgtaag ataacctcat tgccctgtcc
     3961 atcaacaata gcctttttat cacgatgcaa aaacccctgg gcattgactt gtgatataaa
     4021 tgcaacaaca agtaacagtg ttaataattt tttcatattt agtcagattt gttagtccga
     4081 caaaagtaga atagcgcaaa gggcataaac aaaaaagcca ctaccagaat aatataacat
     4141 tactacgagt aaaatactac caaaaataac aagccattgt acatcaatga gttaaattta
     4201 tataaaaaag tggtgattat taaacgcaac tcagacgact tcagaagcaa gaaacagagc
     4261 atttcaaaaa agctcaaata caataaaata cacaattttt cttttgcaaa agcttttaaa
     4321 acaaggattt ttcaaaagaa atcctatcca taaacctttt caaaacattt gtaacaaggc
     4381 atacataaca aatggccacc ataagccata taactatgat attaaaataa aaggtatcaa
     4441 gaaaccaatt gcctatcttt ttggctggag caaaataatg ggctcttccg aagttatttt
     4501 taggaaagtt aaaaactggc tcaaacttac ggatcaagca attgtcaact actgcaatct
     4561 gatcagatga agttttgccg agaacttcat ctgccacagc ctggttaaaa tatctatttt
     4621 taaattttac caattgtcct tcatcatttg aaaactctct taatatcaat ctttgagtat
     4681 aatcatcctt tttctcaata gtattattaa gtatttttac agaaatatca cgtgcatgtc
     4741 ttacataatt tgaagccctg acaaaagcca aactatcaaa cctcgaaggt atcaacagag
     4801 aatcatattt gaaaggagta atttttaatt ctttagaaat agaaactaat tcattagata
     4861 taagctttaa gcttcttgtc aattgggcaa tttttaaact atcatccctc aattgaaaac
     4921 agatatcaag tctgttaagc acttcgggga tccaataatt attgcagtag gtagcattgc
     4981 tctcattcaa ttcgaactgg taaaaccggt ccccatagcg gttataaaca aactgttcta
     5041 ccatcagggc ttcataagcc caacgagaag tcataacatc tccaataaaa ggtacgtact
     5101 tatctgacct tatataaggg tgcaatttgt catattttac aatagttccg ctaagcaaaa
     5161 gctggggaat caaaagcaat gggatagcaa tgtatatagt aacaacagat tttagtccag
     5221 acgaaaggtt taagcttagc atattggcaa aacaagccac agaaaataat acaaaccaat
     5281 actgaaaaaa catcccatgt atttcgagta tccaatgaga aatagcaaca aaaagcccca
     5341 tttgaatagc agacaacata aaagcataca caactttaga attaaaataa gctatgttac
     5401 tcaagttgat aaagctttcg cgctttcgct gacgggcatc gtgtataatt tcttcagcac
     5461 tgctcatcat tcctaagaaa agagcaacaa caacgctcat aaatataaat gccggcacat
     5521 taaggttttc agcaaaagta taaatgccgt tagaagggac aaatcgcgta aagaaactta
     5581 ggataacagc aagtaatgga gcttcaaaaa gagtaaccaa aatatattgg ctatccctaa
     5641 gcttacaatg aatatttcta ttcaagaaca aataaaactg tctaaacacc gatggcttct
     5701 taccattctt attttgtggc aatttttttg acacaaagtc gttttcggta aaagtttgat
     5761 ttttcaaaaa caattcgtac catttttcgg gcaaaacctc tcgcttacct gacataaacc
     5821 cttcaacagt aaggctttca tgttctatta tttcaagaat ctcttccgga ttgattgttc
     5881 cacaccacag gcattcattt tcatcagcat taacgctatg tataacattt ttaaaataag
     5941 caagagagtc tgtggtattg ccaaaataca ccggatagcc acctttatca agcaccaaca
     6001 aacggtcaaa catcttaaaa atatctgaag aaggctggtg aatgttgaca ataacaattt
     6061 tcccatgata ggtcaggttt ttcaatagtt ccataacctt aagcgagtct tttgatgaaa
     6121 ggccggaagt aggttcatcg gcaaaaagca cagctggctc cctcaaaagc tcaagggcta
     6181 tattcaaacg ctttcgctgc ccaccgctga tatatttgtt taaagcattc ccaactttaa
     6241 ggttggcaat ttcgttcagg ttcaaatctg taagcaagtg caatgcttta taggtagcct
     6301 cttcatcagt aaggttacca aaacaaagct ttacgttata gtacaaattt tcaaacacag
     6361 acaattcttc aacaagcaaa tcatcctgag gcacaaaccc cagaaaatca atagctttgt
     6421 cacgttgggc ataaatatcc acatcgctga caaacacatt acccgattgt ggcttcatat
     6481 tcccgttaag gatattcaaa agcgttgatt tgccaacccc tgaacctccc attatagcaa
     6541 taagctgccc cgattgggcc acaaaactaa atttatggat cccgttgcta ctattgccaa
     6601 atctaaactc aatatcacgg gctatcaatt taatctcaga tttcgaatgt cgatatagaa
     6661 aatttccagc aatgtcgcta aaatgcacag tttgtatctt tggacttctt attgcatatc
     6721 ctttttctaa aatataaata caataaggct caataggcct gccatttaaa gtaagcatat
     6781 cattgaactg ctcatgtcta aaaaaatacg actgggtatt ttcatggtaa aacacaagga
     6841 tgaaaccttg caaattttcc cgatgaagga aatgcctaaa actgtaaacc ggattttcac
     6901 cgtaaataat aagaaaagaa ttgtttcttg ctttgttttt taacgaacca aaaacaaaaa
     6961 agaagatttt atcaaaatca tctgcagaaa tatgcagtat ttcagacatt gttgcaaccg
     7021 tatcaagcag tttatcagaa atagccccct cttcatcttt taaaatatcg agtagctgca
     7081 tcaacaggag catcttcttt gtatagtcca gttgatcgtt tatttgtcgg cacaccaata
     7141 ccaactttgt cgaaaggagt gaaatcctct ttagaggctc cgtcttacgg cttttataga
     7201 tatttttaca atgaaagtca aatatctgta tatacctggc agttttttcg gggtttaaca
     7261 cctgtgccag aaaagcattg acaattttac gcgaagaaaa aaaagcagta tccttatcgg
     7321 tatcctggta taacaatttt acattagaaa gaatcgcaaa aagcctgagg attgatttta
     7381 ataccgaatc ggtcatgtaa aagtcataag aaaattagtc tatatcttta aaagcctgtt
     7441 ttgcagctat tgcagcgccg ataataccag cattattgag cagttgcgca ggtactattt
     7501 ttgttctcac atcaaaatgc gagaagattt tattttcatg cttgctaacc cctccaccaa
     7561 taataaaaag atcaggccaa aagaggtctt caatataatg cagatattca ttaaacctta
     7621 acgcccattg ttcccatgaa agctcaagtt ttttgcgggt tgcatcacaa gcataaggct
     7681 cagcttccct atcgcctggc ataattatat gtcccaattc tgtattagga agtagttttc
     7741 catcagaaaa aataaccgtc ccaatgccgg ttccaacagt aataagaact acaacacctg
     7801 atttatctac cccggcacca aatttcatct cagcaaagcc agcagcatca gcatcattaa
     7861 caacttttac aggacagttt gttctggctg taatcaattt ttctacattt agccctataa
     7921 aacttttgtc aatattagca gcagtcttag ctactccatg ttgaacaaca gcaggaaacc
     7981 ctacacctat gggcccttcc catgagaact tctgacaaat ggtttcaata ataccggcca
     8041 catcatcagg cttagcccca acaggagtct caatacgata acggtccgac tgcatctccc
     8101 cggtcttagt attgacaaca gctcccttaa taccagaccc tccaacgtca atacccagga
     8161 tttttttcgt tttcatattt tttaatattt tgtgcggcaa ggtaatacaa gttattaata
     8221 aaaaaaatac ttcaaaaatt aagttaatat taagttttat ttaagttttg aaactacaaa
     8281 atcctcagca taatatttgg cactaagttt ttcgccagtg gctttttcaa tcatatcatt
     8341 ccagtaataa cgtgctcccg gcccaaaaac ctttttcttc agatattccc ctacttcagg
     8401 cctgtttaca taagtaggcc atccagaagc aatggtatct ttcaatattt caacttttat
     8461 atagtggttc aactgtgaag ccagcaattc acccataaga taattgtgat aatagcatgg
     8521 ataagcagct atatgtattt ttgtagccca gtcaggcata ttccgtcctt caggtttgcg
     8581 tatcagttgg tatttttcta ccagctgcca ccaaagagta ttcaaatcct gatcagggtt
     8641 ttcatacatg gctttctcaa agcgatacat cacctgtacc caacgactga ataccaattg
     8701 ttccaatcgg atattattga aaaattgctg agctatttca tcagcttcag cttttgagca
     8761 aagtttcata tctgcaagcc aggcaggatt ggaatacaag cgcccaaaca tcatggcaat
     8821 agcctctgtt gtaagagaat gggccggctg ccgtaatata aaaggcaaat ctctatcaat
     8881 atttttataa tacacggcgt gccccaattc gtgcagcata gtattcatcc acatggcatt
     8941 tgacttgagg ttgcacaata ccctaacatc gccctcgtta tcaatatcaa tacaaaaagc
     9001 atgttgattt ttccctggct tctcatacaa atggcttcgt ttaataatat cattaacggg
     9061 caaaccaata cttttgtaaa aacttttggc tatagtttca atattcttgc cagcaaaata
     9121 tttatcaggc ttaatgttat aaataccagg ggcttgctga aaataacgat tttggtaatc
     9181 ccatggcata aggtcttttt tatttatctt caggcgtaaa gcaaggatag agtctaactc
     9241 atttttgact attataaacg acttgccggt aagctcgtca agctcatcaa acaatcgttc
     9301 aatttcgtta ggatcttgct cgctaatacg caggctcatt tcatgaaaat tgccaaatcc
     9361 aagctgagaa gccagtttat tgcgcaactt aaccaattca ataatatatg cagccaccaa
     9421 tgggcctatc tccttatgtg ctttccatgc agcttcaagt tgtttttggt caacagaatt
     9481 ctttaaaata tcctcaatat cattgtctga ataacttttt ccatcaattt ttgccctaaa
     9541 agcgttgaat ttttcttcaa ttatattttc tttatcaata atttgttgtt gaatatcaac
     9601 atctgcctgg tttgacaaaa acatcaaata caaaacatcc agttcgcggg ctttaacagc
     9661 atcagtaata tcttcagaac ctttaaattc ttcgagtatt ttaaatctgt ctttgcttga
     9721 atgataagcc atgtattttt tcttccattc tgcaagcctg tcaaaatcta ttttatcgcc
     9781 actaatagcc gcttcccagg ctgccctgtt catttcttta tatataggtt tcactaccga
     9841 gtcgtactct gcaacaaact tatcaaagtc actattcgtt ttttgcggat ttgagctaca
     9901 agaaaataac aagaaggcca ataatgctgc gaaaatactg ccaatgaacc tactttgaaa
     9961 cgaacattta cacgtcattt tacactcaat tttatgtaaa aataatataa ttcacctaaa
    10021 aacctgatta aattaaatat acgaaaaacc ttaaatatag cgcaactcag caatcagaac
    10081 ctttaattta cataaaaaca aaagaaaaga agatgaaaaa taaattcata tattttataa
    10141 agtcaaatga tataactttg cacaaaaaaa aatatgtgta gtacttgtgg ctgtggcggt
    10201 gcagaagaaa agggataccg catgtacaaa ccaggagaag tccattctca ccatcatcat
    10261 gaaggcgctc atcatagcca tgggcatacg catcaccatc atgaccacgc tcacagtcat
    10321 gaccatgacc acacacacag ccatagccat gacgatcatg agcactccca cccttcaatg
    10381 ccctctgtta tcgagattga aaaagaaatc ctcgatgtta atgataaaat ggccgaacgc
    10441 aacaggggat attttgaagc taaaggtatt atagctttaa acattgtaag ttctcctggt
    10501 tcaggaaaaa caagtctttt ggaacgtacc ataaaagaat gttcggcaaa acgtaaattt
    10561 tacgtaatcg aaggcgacca gcaaactgac aatgatgccc ggcgcattgc ttctacagga
    10621 gccgttgcct tacaaataaa caccggaaat gcttgtcatc tggacgcacc aatggtacac
    10681 gatgccttgc atgagttaaa acctgaagaa aacagcattg tatttattga aaatgtaggg
    10741 aacttagttt gtccggccct tttcgacctt ggcgaacaaa accgggtggt tgtatttagt
    10801 gtcacagaag gggaagacaa gccgctgaag tacccaaata tgttcaattc ggccaatatt
    10861 tgtgtgataa acaaaacaga cctgcttcca tatcttgatt tcaaaataga gttggcccga
    10921 gaaaacgtaa aacaggtaaa ccccaatgct actattattg aactgtctgt aaaatcagga
    10981 gaaggaatgc aagagtggta taaattgttg aatattaatg attaatgatt aatgattaat
    11041 gagttttcta tttatttttt cgttggtaac taacaattta gcattaatca ttgacaattt
    11101 ataattaact attacttttg gctgacttaa taatgctggt gataagtttt attatttctg
    11161 ttgtttcttc gcaaatgctc ataaattcat gttcagtcaa atattgcgtg gccattaata
    11221 attcaagcca atataaagtc tcattacact ctttttgagc aatagccatt ttatgaacaa
    11281 agtcggcttt gctttcggca tgttcggctt cacgtaccat cgctccaact gcggtaccgc
    11341 tgcgcaataa ttgtttcgaa attatgaatt ctttcttttc tgtttgcaaa tactgaaaca
    11401 gtctaactat ccgaatggcc agtttaaaac ttttatcttt tacaatattt tctttcatat
    11461 cccattatta atcattaaca attaatcatt aacaaaaact tttccagctc ggtataaagc
    11521 ttttgaatag gcaatcccat gacattaaag tacgaaccct ctattttctc gatacctaca
    11581 tagcctatcc attcttgggc accataagca ccagctttat ctgtaggcat ataattggta
    11641 gtatagtaat ctatttcttt tgctgtgaac tctctaaaga aaacctttgt aaaatcagaa
    11701 aaactaactt gttttttatt cgacaacaag cataccccgg ttataacagt atgttgacgt
    11761 ccgctaagtt tttgcagcat atcttttgcc tcatccagat gctcaggttt gccaagtatc
    11821 ttgttatcca gaagtacaat tgtatctgct gcaacaataa tctggttgtc cgtaagcaga
    11881 tgtttgaaat attcagcttt ttcaatagcc agaaattcag gtacagcctc agggttcatt
    11941 gtatctggaa aagactcatc aacacctgat ggtaccataa cttcaaaatc aaaaccagcc
    12001 tcccgcatca gaaaatggcg acgaggcgat tggcttgcca ggattaacct gtaattctta
    12061 atatcatgaa ggagcataaa aacaagacct taacattagt agaataaaac cacccctacc
    12121 cctcctttta aaggagggga aatacagcgt ttttcaaaag aatgcagaaa taagaaaatg
    12181 ccaaatcgta ttaaacattg cagcctgctt aatctttcat actaactggc tgacctgtgg
    12241 tttcgagaac attccaccaa agctcactct caaggtcaat ctttttgcgt ttttcaacag
    12301 ccttaggtat tggcaacaaa gtgaaacaat tgttccagtg gcctaccaca aaatctgttt
    12361 ttccggccat ggcagcatgt acagcatttt gagccaaaac actgcaaaat ttactatcaa
    12421 tagcatttgc cgcagcactg cggataatat aacttgggtc gatatattta atgctaaaat
    12481 tcattccttt ggcctcaaat tcttcagtta ttttctcttt cagatatacg ccaatatcct
    12541 tgtgcttaac gttacctgaa gcatcagggc gctggtcgtt attttcaaag aaatattggc
    12601 ctgcaccttc agcaacaaca atcatagcat gatgacgttc ttctaaacgt ttacgcaata
    12661 ctttaaggaa tccgtgaggc ccgtataagt caaattccat ctcaggaacc aacacaaaat
    12721 ttacttcttg tattgataat gcggcattag cagcgataaa acccgaatcg cgccccataa
    12781 gcttaataag agctataccg ttataggccc cttcagcttc attgtgggca ttcataatta
    12841 tctcattagc aatagctgat gctgtctcaa aaccaaaaga tttatcaatc aggttaatat
    12901 cattatcaat agtttttggg acaccagcta ccgaaatttt caggttacgc ttggtgattt
    12961 cttcatatat agcatgagcg ccacgtaaag tcccatctcc tccaatggta aacaagatat
    13021 ttatgtttag catttcgagg gtatcaacaa ttttgcttac atcctggttg ccacgcgatg
    13081 agccaaggat tgtcccgcct gaaagatgaa tattttcaac catctgagga gtaaggtcta
    13141 ttacatcatg gttataatca gaaataaagc cttcataccc atactttata ccaactatac
    13201 gttttacacc ataacgataa aacaactggt ttacaaggct acggataaca ttgttgagcc
    13261 cggggcataa accaccacag gtaacaatag caaccttggt tttagcaggt tcaaaaaaaa
    13321 gaaactcgcg aggccctgct ttttcaaaag agattggttc ttctcctgtt tctttgcaac
    13381 gtttaaaatt ttctaaagaa gcattgtaaa gaacccggtc gtcatcatta acgtagtcaa
    13441 atacgccatc tcccttatga ttactaaggt gtaacggtga ttttaccgta ccttttccca
    13501 gaacttttac ttcaaactca gcgttcatgc gtttttattt tttatgtctt ttttcaagaa
    13561 ctttaatcac atcttcaatc ctgtagtttt tggctgttag cagcaccaac atgtgaaata
    13621 ccaagtctgc agcttcatta aggaacaaat catcgttttg atcttttgct tcaataacca
    13681 gctctacagc ttcttctcct actttttgag ctactttatt aataccggct ttgaaaagct
    13741 ttgtggtata agagccatca ggcatttcct ttttacgggt atcaattaaa tcctgcaaat
    13801 aagttaaaaa ttttgccgca tcaacatttt tctcatcaaa acaagtatct gcaccggtat
    13861 gacaaacagg accacgagga ttaaccttga ttaacaacgt atcgttatcg caatcaatga
    13921 gcatatcaat tacatcaaga aagttaccac tctcttcgcc ttttgtccat aaccttttgc
    13981 gggtacggct ataaaaagtc accaattttt cattttttgt tttttcgtat gcttcctggt
    14041 tcataaaccc aagcatcaat accttttgag tattagcatc ctgaattact gccggaataa
    14101 gtccgtcgag ctttgaaaaa tctggtttag tcattttttt ttgatttatt tttagttaaa
    14161 aatgaatttg gcataacaaa gttataaaac aatcggatag caaccgccaa ccctaaaagt
    14221 aaaactgccg ggttaaattt ctgcgggagc aacggagaca atgcaataac accaattgca
    14281 aggatgaata tcgccatgca ataattcttt ttccacttca tatttttgac aaatggcaga
    14341 aaaaagttta gcggaaaaat caacaatgcc accatattga aatggagctc aggatgtgtc
    14401 gaggctatac ttaacgtagc aatgaaaatt ccaagaaaac ctgttattag aaagaatatc
    14461 gtatcaaaaa actttacttt agaaggggaa aactgaatac caagcgtaat aagcacaatc
    14521 aacaacatag ccataacagg ggtaaaagaa aaaccttccg aatcggcacc tttagcaaca
    14581 tatagtcttt cagtaaccgg tgcaataagc tgttcatcaa ccttagcctt tgaaagtgca
    14641 tccatcaggt tgtttggcaa gaacatggca tcgtagcccg acttttcatc tgtcattttg
    14701 ccaagtacaa gctcaatgcc aaatctttcc cacttcatat taaacagata aggcgtaaac
    14761 agcatccgaa aggtgctgtc tttagccctg tttgcgttat caaacatgga atattcgcca
    14821 gtagcggcaa caatcacatc gcgtactttt gtagcacaat tattggtaaa gaagcgatac
    14881 ctgtatgccc tgttttcggg cctgtatgca tcgagcaaaa gcgacaccaa catttgcttg
    14941 tttgcttcag gcataacaag cttctgctca tataccgagc gcctttcggc catacaagaa
    15001 cccaaaaagc cctgaaattg ctcaatagac agttggtaat ccagcacccc caaggcaaat
    15061 ttcagataaa agttgggggt cctgaaatca aatgtaccat aattaaacac aatatcatgg
    15121 ccatttttaa agtctttaac acgaatagca gtatgcccaa agaccgaata aagctcatta
    15181 ccaggagaaa cggtaagcat gctcacctgt aaactatcgg acagaacggc agcctttacc
    15241 gaaaaagcag atacgctaac cagcagtgtt aaaactattc tttttataaa attcatattt
    15301 acaaagaaat caagataacc gaactggaat attatgcttg tgtaaataat tctttaaatc
    15361 aggaatggca atttccttga aatgaaaaat acttgcagcc aggccggcat cagcattacc
    15421 tttggtaaat acatctacaa aatgatccat attgccagct ccaccagaag ctattacagg
    15481 aatattaagt gataggctta actggcttaa cgcttcacaa gcaaatccct gctttacgcc
    15541 atcatgattc atagaagtaa ataatatctc gccacatcca cgatcctggg cttctttagc
    15601 ccaggagaaa agctctttat cagttggtat gcggcctcca ttaaggaata cagtccattt
    15661 gccatcttca aacttagcat caatggccac aacaacaaac tgacttccaa agccttttgc
    15721 tatctggtca ataagctgag gattacgtac agcagcagaa ttgatagaaa ttttatcagc
    15781 accggcagcc aaaagccttt ctgcatcaga catttcatta attcctcctc caacagtaaa
    15841 aggaatattg aggttctggg caatacgttt tactaagtca gagaataatt tgcgcttttc
    15901 gtgcgatgca gtaatatcca aatatacaag ttcatctgct ccctgacggc tatattcagc
    15961 ccctaactcg accgggtcgc ctacatttcg gatattttca aaattaatac cttttacagt
    16021 ctgaccatcc ctgatatcca aacatggtat aatacgtttt gccagcattc aatgacgttt
    16081 ttaattaacc accttatctc tccttagtaa aggagagaaa atacaacttt tagtgaagga
    16141 tcttatttct tttatctttt gccttctctc ttttttccct tatcctctgc ttccgtgaac
    16201 aataaagttc tccaggtcct ccagcgtaat acggccttcg taaaaggctt tacctataat
    16261 aatgccataa atatgagctt ttgaaagctc ttcaatctca tgaaggtaag taataccgcc
    16321 actggcaata aaactcatct cgcgaaactg gtgcttcaac cttttatata aatctataga
    16381 agtcccttgc aacattccat ctttgcttat gtccgtacaa agaacatact gaacgccttt
    16441 ttcctggtat tttgtaagaa aatcatccag ctcaatctgg gttatttcct gccatccgct
    16501 aatggcaatt ttatcatcaa gaatgtcggc tccaagaata atgcgttccg ggccatattt
    16561 atcaagccac gacacaaaca actcaggatt tgaaacggct atacttccga ttgtagcttg
    16621 atttgcccca cagtcaaaaa ccagctcaat atcttcatta gtttttatac ctcctccaaa
    16681 gtcaaccatc aagtcagttt tcgaagcaat acgttctaaa actttagcat tgacaacatg
    16741 tttagcttta gctccatcta aatccaccag gtgcaaatgg gttatgccat gatcctcaaa
    16801 catgcgggca acttcaagag ggtcttcgtt gtaaaccttt ttttgagcat aatcaccttg
    16861 agaaaggcga acacacttcc cttcaattaa gtctatggcc ggaataatat tggtcatgat
    16921 atttaaaaat gaaaaatttt cgtaaaaata agaaattcaa acaattaaca aaatgccttt
    16981 aaacaacccg ttctacaaag tttttcaata tagacaatcc tacatcactg cttttttcgg
    17041 gatgaaactg ggtagcgtaa aaatttttat agttcaatgc agcgctgaaa tcgttgatat
    17101 aattggtttt agcaatggta tgttctccaa cctgggcata aaaactgtgg acaaagtaca
    17161 cataagaagc ttcagaaagt ccatataata agggagattt gagttctgtt atagtattcc
    17221 atcccatgtg tggcactttc aacttaggct caaaacgcaa caccttttcc tgaaatattc
    17281 ccaagcatgg agtatcacct tcctcagaat agctgcacat tagctgaaga ccaagacata
    17341 tacccagcac tggttgtgtc aaatctttaa tcagcaaatc gagtttggca tttttcaaaa
    17401 acctcatggt tgtttcggcc tctcctaccc caggaaaaat aaccttgtcc gctgatttga
    17461 ttttttcaaa atcatcagta acctcgcatt caacaccaag tcttttgagt gcaaactcta
    17521 cagacatgat gtttccagca ttgtatttaa taattgctac atgcatgtaa tattcaatta
    17581 atcaaaaacc acagttttgt tattatatac aagtaattta tgctgtaagt gcaatgatat
    17641 agcccttgac aatactattt tttccaaatc tttgcctttt tgaataaaat catcaatact
    17701 atcatcatgg ctaacccgcg ttacatcctg ttcaataata gggccttcat caaggctcga
    17761 agttacataa tgacttgtag cgccaataat tttaactccc ctctgataag ccgaatgata
    17821 aggtttagca cccggaaacg caggcaaaaa gctgtgatga atattaatta tcttattagg
    17881 aaactgattt acaaagtctt ctgagagcac ctgcatatat cttgcaagta caacaaggtg
    17941 tatatcatgt ttgcgcaata attctatctc tttttcttcc atttcccgtt tattttcggc
    18001 tgtaatcggg aaataataaa acgggatttg aaaagcttca caaacaggtt ccagttcctt
    18061 atgattacta ataatcatag gtatttccac attccattca ttagccttat accgggccag
    18121 gatgtcaaaa aggcaatgag acatttttga gacaaaaatg gccattttat gcttataacc
    18181 agagaaatac aaactccaat tcatattgta ccttggtact atcagggtat tgaaataatc
    18241 gccaatttta tcaggcggga taaggaattt ctcaagttcc cactctatac gcatacaaaa
    18301 catgccctgt gagtgatcta catgctgctc aagagtaaca atattgccgt tgttgctatt
    18361 taaaaactct gtaactgtag caacaattcc ctgtttgtct tcacaatgga ttaatagtat
    18421 agcgttttgc tttttcacaa tatgataatt ttataaaact ccttttgttg atggtatctc
    18481 ggcattgcaa gggtcgcggc gaagcgccat acgtatggcc ctggcaaaag ctttaaaaat
    18541 ggcttcaatt ttatggtgtt cattttgtcc ttcagcttta atgttgaggt tgcaacctgc
    18601 agcatcagag aaggatttaa agaagtgata aaacatctcg gtaggcatgt cgcccaccat
    18661 ctcacgttta aactcggcat cccacaccag ccagcacctg ccaccaaaat caatggcaac
    18721 ctgtgccaga cagtcgtcca ttggcagaca aaacccataa cgtgccatac cagctttatt
    18781 gccaatagct tttttaatcg cttcgcctaa agcaattccg gtatcttcaa tggaatgatg
    18841 ttcgtcaaca tcaagatcgc cattcacttc aatagtcata tcaataccag aatgccgtgc
    18901 tatctgcatg agcatgtggt caaaaaattt aagacctgac gaaacatcgc ctatacctcg
    18961 gccatcaagg tttatcttaa tatttacgtc agtttcagag gttacacgcc tgatatgtac
    19021 aattctttcg ggcagggcaa gaaagctata aatatcctcc caatcttcag taaccagggc
    19081 gcagtaattt tctaaacctg cattttttat atcttcatat ttatcttcag caccgtaaac
    19141 aatagctttg cagccaaggt tacgggccaa cataacatca gacatccggt cgccaattac
    19201 ataacattca gacatatcgt aacttccgtt catatatcct gtaagcattg ctgtcccggg
    19261 cttacgtgtt ggcttgttat cttcgggaaa ggacttatca ataaatacag cgtcaaaaaa
    19321 gatatcttca ctttcaaaaa cctgcatcat cttgccttgc accttgtcaa agttttcctg
    19381 gggatataaa ggtgtaccca agccatcctg attagtaacc atgaccattt caaaatcaag
    19441 gttttttcgg ataagagaaa gcgatcggat aactttaggc atgaattcta attgttccag
    19501 ggtgtccacc tgttttgttt caggaggttc aacaatcata gtcccatcgc ggtcgataaa
    19561 caaagccttt ttcttcatta tttgaaatta tttagcagtc aaatttaacc caaataaatc
    19621 atatagtttg gcaaaacatt gtctgctgat gattaacctg ggtttaatga atattgtttc
    19681 agtgccatac ataattgctc attttcgcct tttgaaccaa ctgtaatacg gatacaacca
    19741 gcacataaag caatctttga gcgattacga acaactattt tattatcaat caaatattta
    19801 tacaaacctt ccgcatcatc tacttttacc aaaacaaaat tggcatcaga agggaaaatt
    19861 tttttaatac agctaatatt agacaattgg tcaaccaaaa gctttctgtt tgctaaaatt
    19921 tcttttaccc atttattctt ttgctcgtca ttattgagaa ttttcaaagc atactcttgg
    19981 gtaagagcat ttatattgta tggatatttg attttattga aaacactaat gatttcttta
    20041 gaagcaaaag ccattcccaa acggatagcc gccaaacccc aggctttgga gaatgtctgc
    20101 aatattacca aattaggata ctttgaaagt tttggcaaaa agcccacatg attagaaaaa
    20161 tcaatataag cctcatcgag cactacaatt ccttcaaagc cttcgataat tttctccatt
    20221 tcagatacat ccaaataatt ggcactcggg ttgttaggtg aacaaaggaa aacaagacgt
    20281 gtatttttgt caatagcagc aaaaaccgat gttgatacaa gttcaaagtt ttcactaagc
    20341 tttactgtgc gatattccac attattaata tcagcagcca ctttgtacat gccataagtt
    20401 gggtcaatgg ccacgatatt gttctcgcca ggctcacaaa aagccctgat aacaatatct
    20461 atgggctcat cacttccgtt gccaagaaaa atattcccgg ctggcacgtt tttaatcccg
    20521 gcaatttttt ctttcagaag ctgctgaagc gggtcgggat acctgttata aggagcatta
    20581 aacgggttct cgtttgcatc gaggaataca cttgcctcgc cctgaaactc atcgcgtgca
    20641 cttgagtatg gctttaggct ttttatattt ttccgtaaaa gattatccaa gtttatcatt
    20701 gcaatattta ttaacaacac taccaaggcg aagggttaca gcatttttat gcccgtcaag
    20761 ctgttcaagg gcagccattt tttcaatggc agggccaata ttgaaaatac cataatcgga
    20821 aatatgctga taggtcacct tcttaacaaa actatcaagg tttaccccgc tgtacatttt
    20881 ggcatagcca ttggtaggaa gggtatgatt tgtccccgaa gcataatcgc cagcactttc
    20941 gggggtaaga tggccaagaa acacagaacc agcattgatt accttcattc caagttccat
    21001 agcattatca gtagcaataa tcaggtgttc cggggcatac tcgttgacga tgtccatcat
    21061 ttcatcattg ctcggggcaa tgataacacg gctattgcct aatgacacag tagctatatc
    21121 cttgcgggga agggcagcaa gctgtttttc gacttctttt tcgatagcag ggacaatatt
    21181 ttcatcagtt gtaaaaagca atacctgact atctttgcca tgttcggcct gagacaaaag
    21241 gtctgaagct acaaaagcag gattgcttgt agcatcagca ataactgcaa cttccgaagg
    21301 accggcaggc atatcaattg cggtacctgt acggttaacc aattgcttgg cagcagtaac
    21361 atattggttt cccgggccaa aaattttata tacctgagga atggaggcag ttccataagc
    21421 cattgcacct atagcctgca cgcctccaac tttatatatc ttttttattc ctatgagtga
    21481 ggctgtataa agaattgagg ctgctaattt accatttttc atcggtggag aacacacaat
    21541 tatctcttta catccagcca gcattgccgg aatcccaagc atcaacacgg tagaaaataa
    21601 aggagccgta ccccccggga cgtaaagccc aactttttca atagcaacag ccctttgcca
    21661 acaggttacc ccgggagtcg tttcaacttt cttttcaaca actttttgct ggaggtgaaa
    21721 tttttcaatg ttgtttttgg ccaaaataat ggcctttttc aacttttcgt caatctggcc
    21781 gacagcttca tcaatctctt caggagtaac ctgaagtgaa gaaatcgaca ctccgtcaaa
    21841 tttagcgttg tattccagca cagccttgtc tccattggct tttacatctg ccaaaataga
    21901 agaaacagta ttttcaagca aactattgtc aatggccgga cgctttagca catctttcca
    21961 ttttgacctg tcgggatatt ttattaattc catgcttttc aattactaat tacgaatgac
    22021 taattaccga ttaatagttc tcattaacca ttaaccatta acaatttacc attaacaatt
    22081 taccattaac catttgctta aacaatcatc ttctcaattg ggataaccaa aatcccctgt
    22141 gcccctttat cctgaagctt tccaataata ttccagaaat ctttttcagg aactaccgaa
    22201 tgcagcgaga cccatccttc gtcagccaaa ggcacaattg acgggctttt cattcccgga
    22261 ataatggcaa taatttcatc taagttcttt ttgggagcat tcaaaagaat gtatttattt
    22321 ttcttagctt tctgaacgct tctcattctg aatatcaaat cttcaagaat agcttgcttt
    22381 gaagcagaaa gatttggtgt agagataagc actgcttcgg acttcattac cacttcaacc
    22441 tctttcagat tgttgctaaa caaggtactt ccagaactga caatatcaaa aatagcgtca
    22501 gccaggccaa taccaggggc aatctccaca gaaccactaa ttacgtgaat atctgcagta
    22561 atatttttct ctttcagaaa ttttgacaaa atcacaggat aggtagtggc aatccttttt
    22621 ccctgaaaat attcaaggcc tttgtattca tgggccttag gtatagcaag agagatacgg
    22681 cattttgaaa aaccaagccg ttcggtaata acaacgtctt tgccggtttc gacatacaca
    22741 ttttcgccaa caacgcctac atcagccact ccatcggcaa catattgagg aatatcgtca
    22801 tcacgaagat ataaaacctc aacaggaaaa tcaggtgaat ctaccaacaa acggccacta
    22861 cctccggtaa ggccaattcc gcattcgtta aataattcaa gtgttttttc gctaagccgt
    22921 cccgactttt gtaccgcaat tctcaatttt tccattttat cttgtttttc tgtttgttaa
    22981 cttattaaaa ctaaaaaagg cctgcagaac cccgcaaacc tttccattat attgatgtca
    23041 tagattttac tttttctgca tatacaatat cattgcacag gatgatgatg cctgtatgca
    23101 tgatgaattg tatatgtgtt tttcctcatt gtttaattat tatttcgaga caaaggtaat
    23161 ttttttttaa taaaacataa aaagctgaca aactttttta atggctaaat ggctctttaa
    23221 acaagtttta gacatttttt ttaggtctaa cctaatttta atttcatttt aatacaaaaa
    23281 cggatttagc accatacttt tgtatcgtct tgattacgca acacgcaaga ctgataacat
    23341 ttaatatatt cacgttctgc aaaaaagagg agaccgcaac atctcctctt tttatttttt
    23401 attttgcttt tcgcaagggc aaggtacagc tttatgtttg gtcagcccgc gttttgaaca
    23461 tgcagtaacc attaacagac atagcataag cactacaatt ctactcaaac attttctttt
    23521 aaaaatcatt tttcaagtat ttataaaagc aaaattaact gaattggaat ttttaagcat
    23581 aataaacctt catcctttca tcaaatccca taattttcgc aatataacct gcattagctt
    23641 ttgaagttag catttcgagc aatagcagcg ctacaaatat tgccgtagaa ggattccacg
    23701 aggtaataat attattatcc acaactatag gttcgtttat cacatcagcg ccataagttc
    23761 caagcaattg ctgtcgtatt ggcttttggt tataagttgt agctttttta cctttcaaaa
    23821 tccctgcctg acctaacaaa agtgcgccaa cacaaataga agctatcggc ttattttgtt
    23881 tgtaaaactg ttgtatcaat tcctgcacat caacactata agcttctcta taaaaatcat
    23941 actcttcaaa tcccccggga acagcaagtg catcaaaatc ttctgctttc acctctttta
    24001 tcaaataatc aacgacaaat ttctggtcaa aacttgaaac aacttctttt gtcaggccac
    24061 aggtaaataa tttggttgtt tggtcgccat caacaaggtt ccaaccaata acatcaataa
    24121 aaacacaagc ttcataagtt tcaaagccat gaggcaaaag gagaaggact tttttcatgt
    24181 ttttttttat tttttcaggt ttaatgcacg ccacatgatt caaaaaatgt accaaagata
    24241 aaatgaaatg tttacttgaa aatcaacact ttaaccacaa aacactaaaa ctttcacttc
    24301 aaattttaca atgttacacc cgacttaaaa atggcaattt ccctataccc tgacttttcg
    24361 ttattgacaa gttttccgtt ggcaatgtcc agaatataag ccgtgaattc tttgagtaca
    24421 tcatcaatgt ttctgccatc ggctatttgt ccggcgttga agtcaatcca gtgtggctta
    24481 ttcagaaata aaggagtatt ggaggaaatt tttgtagtgg gcacaaagct gccaaacggc
    24541 gtaccacggc ctgttgtaaa aagtaccatg tgacaacctg ccgctcccaa agcggttgat
    24601 gaaacgaggt cgttgcccgg ggcgctcagc aagttcaggc ctttaacatt aatcctgtcg
    24661 ccatatttga gcacatccga aacctttgct tttccgcact tttgtgtgca acccaacgat
    24721 ttttcttcaa gtgtagaaat tccaccttct ttgttgcctg gcgaagggtt ttcatagaca
    24781 ggctggttgt tttgtataaa gtatgctttg aaatcgttga tcagatggac aatattttca
    24841 aatgtggctt tgtctttggc gcggttcatc agtattgttt cggcaccaaa catttcgggc
    24901 acctcggtca gcacggttgt cccaccctgg ctgattaaaa agtcgctgaa caagccaagc
    24961 aatggattgg ctgttatgcc agacatccca tcagagccac cgcattttag cccgacttta
    25021 agctcagaaa caggcacgtc tgttcttttg tcctgaatag caaggtggta cagttctctg
    25081 agaagcttga gcccttcttc gtgctcattg cccacctttt gtgtttcaag aaacctgatg
    25141 cggtctgtat cgtaattgcc caataattcc ttgaatgctg aaggctggtt gttttcacaa
    25201 cctaagccta agaccaggac ccctccagca ttggggtgca aaacgatgtc acgcaggatg
    25261 aggcgagtat tctcgtggtc gtcgcccaac tgcgagcaac catagttatg tttgaatatt
    25321 tcaatggcat ccaatgcaga agtgcctatt tcggccttca gcgcctggag cattttttcg
    25381 ccaataccat ttacgcagcc tacagtgggc acaatccaca gttcgttgcg aatacccaca
    25441 tctccgtttt tccgtctgta acctttgaat gtcagggctt ctttgggata tacattttct
    25501 gctatttgct ggtcgtactg gtattcgagg attccctcaa gcttcgtttt aatattatgg
    25561 gcatgaacat gttgcccggg acgaatttca gttaaagctt tgccaatagg gtagccatat
    25621 ttgataatgt ccgaaccttc agcaataaca gacaatgcaa acttatgggc ccttggaatg
    25681 gcttgttgca aagtgatttc tttattgttt attacaaaca tctcgcccgc attaaggtct
    25741 gtcaatgcaa tggctacatt atctgatttg tcaatgatgg taaattttga catagctaca
    25801 ccatttgata ttattatttt acaagctctt taacactttc aagcatgccg tctttcaaaa
    25861 tttttgaaag gtattggcat actttttctt ctaatccttt ggttgaagta aggttctgtc
    25921 cccagaacgc ttcattttta agtgtctcgt gaacgatagc atctgtattg ccagaaaaat
    25981 tcttccacac gtttgcgaaa aactgcatga caggttcatc gtctttgact gctattgcct
    26041 tgcctttgta ttctcctttg taaaaggcta tcagggcagc taaggagaaa acaatatttt
    26101 cgggcagttt gccgaactta tggttataat cgagcaatgt aggaagcagg cgggatttga
    26161 atttcgaaac cgaatttaac gaaatgctca tcaaggcatg gtttacaaat gggttgcgaa
    26221 acctgtccaa tacctcgtct gcaaaacttt tcagttcttc tgccggaagg tcaagcgttg
    26281 gaattatctc gttgttgatt gcttgaagga tgaattttcc aatcacaggg tgttctacag
    26341 cttcgcggac atactctatg ccagataaat aagccactgg catcattaaa gtatgcgggc
    26401 cattgagtaa acgtactttg cgggtacgat atggtttaac atcattgaca tacagcacgt
    26461 tcaggttggc tttgtctgta gggaactcat ttttaaccca ctgcgggcct tcaatcaccc
    26521 aaagatggaa ctgctctgca tcaacgcaca tgttgtcttc aatgtttttg gtattggcca
    26581 gctctaaagc ggtgtcttta ttaaagcctg gcactatgcg gtcaacaagg gtgttacaaa
    26641 atgtacagct tttttcgagc cattctttaa atgcatcagg gaggctccac aatacacaat
    26701 acttgtaaat acaggctttt aactcgtttg cattattttc tatcaactcg caaggcagga
    26761 tgataaagcc ttttgaaatg tcacccttgt aaaaagtgaa cctatgatat agcaactgag
    26821 ttagcttggc cggaaaagag cttggtggtt gcatctccaa tttgtcgttt tcgtcaaatg
    26881 ctatcccggc ttcggttgta ttggaaatga taaaacgcat tgtaggaatt tcggctaatt
    26941 taatataccc ttgataatca acataagggt ttatacagcg cgaaatggcc gagttaagct
    27001 ttatgtcttt tacgcccttt ccgtttttaa ttccctttag gacaacatga taaagacaat
    27061 tttggctatt aagtttatcc actaacccat ttttgatagg ttgtacaact acaacgttac
    27121 tgtcaaatcc ggcattatca ttcatctctt gtatcatcca atcgacaaaa gcccgtagaa
    27181 agttgccttc gccaaactgg ataattcgtt cttcagtttt taattgctta tccatgttta
    27241 tattatttga aatcattttt tttgcttttt tatagaagaa tcacgtacca ctaaggttgt
    27301 tttcattaca acctgccgtg gtgtacccgg agttttgctt tccaattggt tgataagcag
    27361 gcgtgccgct tcctgtccca tttcaaatgt ggggtgcata acagatgtca gctcggggtc
    27421 aacaatagag gcatgaaatt catcggtaaa cccgacaaag gcaatatcat cgggaatttt
    27481 gtaaccacgc ctttttacct ctttcatagc agaaaaggct acagtgtcat taatagaaag
    27541 tatggcatct ggcttgactt tcaaggccag tagtttttga gttgcaacct ttgccccttc
    27601 aggggtcatg ttgcatgaag ccagtaaatc attgctaatt ttcaggccac aatctttaag
    27661 gccttctata tagcctgcca aacgctcttt tgaaatgttc aggtggttgg ggccgtttat
    27721 aaatgcaatg cgcgtgcagc cattttcgta aaaatgcctt gtaacgtttc gtgcagcttc
    27781 aaagttgtcg gcaaccactg acgaaatctc gcttgtacgg cataccctgt caaaaaagac
    27841 cagcggtgtg ttatcttcca gtatcttatc aaaatgttta taatcggctg tttcttgcga
    27901 aaggcaaaca ataagcccgt caactctaag atttagcaag ttctcaataa tattttgttc
    27961 cttctgataa gattcctgcg atgaagaaat gacaatcgaa taaccttttt ctttcgtata
    28021 gctttcgatg ccactaataa cagaagcgta gaaatatgta acaatatcag gtattacaat
    28081 gcctatcgtc tttgattcat gcttcagcag cctgatggca taagggttag gtttgtaatt
    28141 cttctgtctg gcaagctcct gtaccttttg agccagttcg ggactaatgt ccgggtggtt
    28201 tttcaaagca cgcgaaacag aggaaatcga tattttcaat tctttcgcaa gatcttttaa
    28261 agaaacatga actttttcca tcagttgcta ttttgcgtaa aaaatgttgt acaaaaatat
    28321 gtttattatc aatccaataa gccgtttcaa tgttttgttt ttccgcaaag gtttgcgaaa
    28381 agctttgcgt tgaaataatt gaaaaataag tttttaaaga ccatttacaa atatacattt
    28441 gcgatgatag attttttcaa aaacgataaa agcttatgtt atcaaaccca gtcaacctgc
    28501 ctaaagtaat atttggcacc agcgccttag gcaatttata tgtagaacaa cccgaaagtg
    28561 ttaaaacaaa aataatcagc gaatgtattg aaaagtcaaa acttccggtt gtgtttgatt
    28621 ctgccggcaa gtatggggct ggattggctc ttgaaacact aggtaaaata cttacaaaac
    28681 ttgacattcc cgctgaggat gttttgatta gcaacaaact gggctggttg cgaactcctt
    28741 tgttgaccga tgaacctact tttgagccag gcgtgtggaa aaacctgaaa tatgatgcgg
    28801 ttcagcgtat cagctatgat ggtatcatcg aatgtttcga gcagggcaat agtttactgg
    28861 gacagaaata tacccctcaa ttggtttcgg tacacgaccc cgatgaatat ctggctgttg
    28921 cagattctga cgcttcatac aaaaaccgct ttaatgatat tattgaagct tatcgggctt
    28981 tgtctgacct caaaaaacaa gggaaagcaa cagctattgg tgttggggct aaggattgga
    29041 aggtgattcg cgaattgtac aatcatatac agctcgactg ggtgatgttt gccaatagca
    29101 tgaccatcat gaaccacccc atcgagctta ttgagtttat ggagaaattg cgtaaagatg
    29161 gcgttaccat catcaattct gcagtatttc aggctggatt tttgattggt ggcgagtatt
    29221 acgattaccg caagattaag cctgacaccc cggaaaatat cagaatattc cgctggcgcg
    29281 aagacttcca ccagctttgc agaaagtata atatttcgcc catgatagct tgtgtaaact
    29341 ttgccctgag ggcacccggc gttcattcaa tttctttaaa tacctcaaaa ccggagcatg
    29401 tagaaaaaaa tgtagcttct gccaatgcca ttgttccgga cgatttttac caggacatga
    29461 aaaaggctgg tttgattaat gattactgta cctttgttta aaatactaac actaaacgac
    29521 tgacaaatga gcactaaaaa actaactaca cccgaatatt tgttgccttt tgcgctgatt
    29581 accgcattgt ttttcttgtg gggcatggca catggaatgc ttgatgtttt aaacaagcat
    29641 tttcaggaga ttttgcagat ttcaaaagca aaatcaggtt tgatacagtt tgccatgtat
    29701 atggcatatt tctctatggc tttacctgct ggttatttca tgaagcgttt tggttataga
    29761 aaaggtatta tccttggttt gtcgttattt gctgttggag ctcttatgat tgctgctact
    29821 acaggatttg aatcttactg gatgttcctt attttcttgt ttattttggg ttgtgggctt
    29881 gcaacattag aaactgcagc caatccatat acaacaaaac tggggccttc tgaatctggc
    29941 gaaaggcgta tcaatttttc tcaatcattc aatggtttag cctggactgt tggcccgctt
    30001 attggagtat tcatttataa cagcaattct agcggcgacg gggagaagct taaatccctg
    30061 atgttaccgt attttgtcat tggacttgtt gtattagcag tggctttact ttttgtattt
    30121 actaaacttc ctgaaatcaa tgaagattac gatgagcctg catcagattc catatcaaaa
    30181 aagcctttaa taaaagacag gcatttggtt ttgggagtta tcgcccagtt tgcttattgc
    30241 gcagcacaaa caggcgtgtt tagttatctt atcaactatt tgactgacaa aggccagcca
    30301 atacattttg atacttcgta tggcccgtat ttgttgtctg ttgggtttgc acttttcatg
    30361 attggccgta tgtcgggcag ttttatgatg cgatatttca atccatcgcg tatgctggct
    30421 ctgtatgcat tgatgtgcgt acttttgttg cctgtagttt gtgccaacat tggatggttt
    30481 tcaatcatcg cattatatgg cattttcttt tttatgtcca tcatgtttcc tactattttt
    30541 gccttggcta ttaaagattt agggccaaaa accaaaaaag ccagctcgta cattattatg
    30601 tccatcgttg gaggcgcagt tttccctcca ctcatgggat atatagccga cgttacccat
    30661 tcgatgtcta ttggttttct tgctcccatt ccattttttg cctacatttt ttattatgca
    30721 ctttctggcc ataaaatact aactaccaac taaataaaat atgaaacaga ttaaattatt
    30781 ttttttgttt ttggctatac cattgttaaa tggaagaatc atagcacaaa gcccaaagca
    30841 ggtttatcaa cctacctttg aatccttgga aaaagtaaat cctgttcctg aatggtttaa
    30901 ggatgctaag tttggcattt atttccattg gggagtcttc tctgtacccg cttacgctaa
    30961 tgagtggtat ccccgaaaca tgtatttcaa aggttctaat gaaaacaagc accatattga
    31021 aacctatggc gacattaccg aatggcctta ttctaacttc atcaccgggg caaaagataa
    31081 aaaagggaac tttgttcaat ttgcaccaaa gctaaaatca gaaggcggaa aattcgaccc
    31141 tgaagaatgg gctcaattgt ttgcaaatgc cggggcaaaa tttgccgggc ccgtagctga
    31201 acaccacgat ggctattcta tgtgggcaag caaggtaaat ccttggaatt caaaagatat
    31261 gggcccaaaa ctcgatttgg ttggcctgtt tactgatgct atccgtaaaa ggaacatgaa
    31321 agtattcctt tctatgcacc atgcttataa tataaccggc tattttgaag ctgttcctgt
    31381 aaccgatgac cctaaacttc aaatgttata cggccaacag ggaaaagaga aaaatgaagc
    31441 cttttggctt gccaaacatc aagaaattat tgatttttat aaacctgata tcatttatca
    31501 ggatttcaac ctgcatctga tttcgcaacc tgtcttgtta ggctttttgg cctattatta
    31561 caatagcgct gccaatcaaa acaaagaagt ggtggccact ttcaaagatg ggttgaataa
    31621 aaaatgtgcc cttttggatt acgagagagg ggggcctcag gatattactg acaattattg
    31681 gcttacagac gatgccatca gctcgtcaag ctggagttat accgaaggca ttggttacta
    31741 ttccaaaact gcactcttgc acgcatttat tgacaggatt agcaaaaatg gcaatatgat
    31801 ccttaacatt tcgcctaagg ccgatggtac aataccacag gaacaaaagg atgtattgct
    31861 tgcatttggt gcttggctca agaaatatgg cgaagctatt tatgccacac gtgcatggga
    31921 gaaatatggc gaaggcccaa caaagatggg tgctgctcat ggcgttttta ctacacctgt
    31981 gactggcaca actcaagata tacgttttac ccgctcgaaa gacaatacaa cgctttatgc
    32041 tattttgctg gattggaaaa agggacaaaa agaaactgta attaaaacct tatcttcaaa
    32101 cagaattaat ctgaaaagcc ttagatctgt tcaacttata aatggagaag ccggaaaata
    32161 cctgacattg cagattaagc aagatacatc agggctgaaa gtctcattgc ctgagcattc
    32221 ttttgacgac atggcttatg tgctaaaatt tacttttgat ggcactatcc caactcttga
    32281 taaatataca gatttaaata cttctcctca ttactacatt gttccgggca ataataccgg
    32341 cgcattggtg cttggcgccg atttgtcgct tagcggaaaa aggaaagatg cagccaacca
    32401 atggaaattg gaagctttgg gaaaaggttt ttacatcatt cacaaccgca acaatggcaa
    32461 caaagtgttg gaagtgaaaa atgataaaag tattgccatt tctgatattt cgaggaaaga
    32521 ccaacaggtt tggaaaattg aaaatacctt caacggattg ttaaaaattt caaacaaaca
    32581 atatgcagag gttcttttaa ctgtaaatat tgataaagag acggtatcgg gctggaaagt
    32641 aatggaagta tgcgaaacta agcaggaggc tttcagaaac aatagcattc ccggcacaat
    32701 tgaagtggaa gattttgaca aaggctgtcc tggcgatgca tattacgaca gagatgaagc
    32761 taacgccgga ggacaatatc gaacaagcga aggaattgac attgaaaact gtgctgccgg
    32821 tggtttcaac attggctgga ccaacaatgg cgaatttatg gcttacactg tcacggttag
    32881 caaagcagct acttatcaag cctcgttttt tgttgcaact ccatcggata ataccaaact
    32941 tcatctcgaa tgcgatggag ttgatgtgtc tggcgttatt ctcgtcccta ataccggtgg
    33001 ataccagaat tggcaggcag tgcaaaaaag tgtcaagctg gaggccggac agcatgtact
    33061 aaagcttgtt gtggatgaag ctccggtgaa tatggataaa atgatttttg aagaaataaa
    33121 atagaaataa acatggaact ttgtcaccag gggacattta agcgttattg taaaactatg
    33181 gaactacgca atgatgccca gctaatagaa gcctacaaaa aagctcatgc caaaggtgcc
    33241 gcctggcctg aaattacgca agggatgaaa gaggttggta tcattgacat ggaaatatac
    33301 ctctttggta ctaagctttt catgattatg gatacggtgg ctgattttga ccatgacaaa
    33361 gctatggccc aattagcaac caaacctcgc caaagcgagt gggaagctta catgtcgcaa
    33421 tttcaggaca catcggccga agctacggcc gaccagaaat ggcagttgat ggagcggatt
    33481 tataaaatgg atttttaatt taatatcaaa tagaaccaag gggcaagtta ttctgaatga
    33541 tatttttttt gttctttact ctccttgttt cttgctcttt tttattttta ttatatgaaa
    33601 gcatttgcaa ttaaagaaaa aggtgttgta gcagacattg aagttgctga accacaagta
    33661 agcacaactg atgtgttgat agaggtaaat tacattggtt tgtgcggtag cgaccttaat
    33721 tcgtatcgtg gtcttatgcc attggtaaca ttaccaaggg ttcctgggca cgaaataagc
    33781 ggaattgtta ttgataaagg aaaaagtgta cctacttcaa ttcaaatagg cgacaaagca
    33841 acagtttcgc catataccaa ctgtgggacc tgtcctgctt gtcgtgccgg acgtcctaac
    33901 acttgtgagt ttaaccaaac attgggtgtt cagcgcgaag gagctttgac cgagcgcatt
    33961 tcagttcctt ttgaaaaggt ctttgtcagc aaaaaactta ccttggaaga attggcgttg
    34021 atagagccca tgagtgttgg ctatcatggt gcaaaccgtg gaaatgtctg cgaaacggac
    34081 actgtattgc ttttaggttg tggaaccatt ggaatggggg cattggttgc tgctgcccgc
    34141 aaaggagcta ccgtaatagc tgttgatata gacgatacaa agttggaaca agccaaaaag
    34201 tttggagcta cttatactat caattcgcaa aagcaggatg ctattgctgt cattaagcaa
    34261 ctgaccaaca acgagggtgc cagtgttgtt attgaggctg ccggaactca ggctacttac
    34321 aaattagcgc ttgacgctgt ttgctttgcc ggaaggattg tttttattgg ttatccaaaa
    34381 catgaagtca gctttgatgc tccattattg gtcagaaaag aagtgaatat ctatggctca
    34441 cgtaatgcac ttcgtgtttt cccttcagta atctcaatgt ttgaaaaaaa cgaaaggcct
    34501 tttaccaact tgataaccaa ggtgttttcg tttgagcaaa cccccgaagc ttttaggttc
    34561 tgggatgaaa atacaggcaa agtctctaaa atactaataa aagtaaaata gttcgtatga
    34621 aacactcata ttttgcaagc acaacttaag cgaaacattt ttatgcacga aatgataata
    34681 aaatcaatta catgggtgtt tccccgccaa agacgggaca agctatcata cctttaaaat
    34741 taattttatt tatgattaaa ctcgatgctc atcagcattt ctggctttac aacccggatg
    34801 aatatgcctg gattggcgag aatatgaagg ttttgcaaaa tgactttttg ccaatgcaac
    34861 ttcaaaaaga gttagatagc atgtcatttg atggaagcat tgtggtacaa gccagacaaa
    34921 ctcttgaaga aacagaatgg ctgctaaaat tagcttctga atacgatttt atcaaaggcg
    34981 tagtgggatg ggtcgatttg tgctccgacg atattgaaaa ccaactgaag aaatactccg
    35041 ataaccctaa gtttgttggt gttcgtcatg ttgtgcacga cgaaccggat gactatttca
    35101 tggcacgggc agattttcag catggaatca gcctgctgga aaaatacaac ctcacttatg
    35161 atttgctttt gttccctaaa cacttgcctt tagctacaga attggtccga aagtttccca
    35221 atcaaaggtt tatccttgac catattgcaa aacctcttat taaaggacaa gttaaagaac
    35281 cttgggcttc agaaataacc aaattggctc aatggccaaa tgtttattgc aaactatcag
    35341 gaatggtaac cgaagcagat tggcaaaact ggaaagcccc tgactttgct tattatttgg
    35401 aaactgtata tcaggccttc ggctcaaaca ggcttatgat tgggagcgac tggcctgttt
    35461 gcactgttgc cggggggtat caacaagtca tgtgtgttgt aacagatttt ataaagcagt
    35521 ttgatgatga tgtgcaaaag aaaattttag ggatgaattg ctatcgattt tatctctcta
    35581 aaattcagta ttaaaatcac taaacaatac atattttatg tagtcccaaa aatgcggctg
    35641 ccgtttatgc atattgcttg attttttagg tttatatttg cgtccattaa tataaaatat
    35701 gaaacgagca tgattcgtta aacacaaaca aagaagaaaa ttcatcatgc gagttttcct
    35761 tcggagagcc cttcggggtt catccgacct tatttataaa attatttacg aatgaaaaaa
    35821 caagttttgg ttacaggcgg aacgggttat ataggttctc atacggctgt agagctaatc
    35881 aatgaagggt ttgatgttgt tattgtggac aatctttcca attcatcatt ggacgtatta
    35941 gatggcatcg agaaaatcac gggggttaag cctgattttg agcaattcga ccttaccgac
    36001 aagctaaaaa ctgatgcttt ttttaaaaaa tataacagga tagaagctat cattcacttt
    36061 gctgcttcaa aagctgtagg tgaatctgtt gaaaaaccta ctctttatta tcgcaacaac
    36121 ctggtttcgt tgttgaacct gatagataat atggcagcaa acggaattaa acatattgtc
    36181 ttttcttcat catgtacagt gtatggtcaa cctgaaatac tgcctgttac agaagacgct
    36241 cctattcaaa aagctatgtc tccttatggt aataccaaac agatttgtga agaaatattg
    36301 caagacagca ttgtggccaa caaagcgctt aatgttattg cacttcgtta cttcaaccca
    36361 ataggggcac accctacagc gttgattggg gaacttccgc ttggcgtacc agccaacctt
    36421 gttcctttta ttacccaaac agctattggg ttgcgcaaag aacttagtgt ttttggcaat
    36481 gactataata cccctgacgg ttcatgcata cgcgattata tcaatgtggt tgatttagcc
    36541 caagcccatg tggttgccat caaccgtatg attaaaggcc tgaaccaaga tgcatttgaa
    36601 gtgtttaata ttggcacagg caatggaata tcagtatttg aaataattaa taccttcgaa
    36661 aaggttacag gcgttaaact taactataaa gtggttggca gacgtgccgg cgacattgaa
    36721 aaagtgtggg cagacacttc aaaagcaaac aatgttcttg gttggaaagc aacccgtact
    36781 cttgacgaaa ccctggtttc cgcctggaag tgggaacaat atattagaaa caaaaaataa
    36841 actgataaaa ctcgtttttt taacaaaatt tcaccgccca aaatagggct ttcatcgatt
    36901 tatgttttgt ttgggagaaa atgagaaata actttgcacc taatttctaa aactcccgat
    36961 atggttgctt tattattact gtcattattg ctttctggtt ctccgtctca tagcaatgct
    37021 gtcacaaact atgaagcaag acagacaatt gacttgcatg gcacctcggt tgaacataaa
    37081 gttttagacc aaaaaggata tagcattcat tattttattt ccggaaataa aaccggcgaa
    37141 ctaatcatat ttttacatcc cgcttttgca gaccaccgaa gctttgacaa ccaggtaagt
    37201 tttttctctc acgaatacag ggtaatcatt ttagacatgt tagggcatgg actttcaaag
    37261 gtcggaaaat caaaagacaa gattgattgc actgttgaac atatcgattc tattatgaaa
    37321 atagaaggtt acacaaaagc ccatatagtt ggtgtttcag caggctcttt gttagcccaa
    37381 tattttgcct tacaatatcc acaaaaagtc ctttcactca cagtacttgg agggttcaat
    37441 atcaacgaaa aaaataggga aatttcaaaa agccagcaca ttgaaaaagt ggaacttgca
    37501 tttaaagctt tattttcgat gaattcattc agaaaagaag tgtcgagaac taatgtaacc
    37561 agccttgagg agcaaaagaa atattacgaa atgtccagcc tttttactag aaaatcattc
    37621 aaagccatgt ctggaatggg aaaaatcctt aaaaacaggg aagatgtgaa acatgaatat
    37681 ccactgatga ttatggttgg cgaaaaagac attaacttgg cccataatat caataatcag
    37741 tggcacgaag aagatccaga tgcaaaatat tgcataattg aaggtgcagg acattgtgcc
    37801 aacatggaca accctgatga attcaataaa accttgctta agtttttaaa aaaggaaaac
    37861 taaattattg acttgttttg gtttatgctg taacttcgta aatcatatat atatttaatg
    37921 aaaatagcta tttttcttta tgaaaaagtt acagcccttg attttgtggg cccttatgag
    37981 gtactaagcc gcatccccaa tactgaaatt tgttttgttg gggcagaaaa aggccaaatc
    38041 gctgatgccg gaggactgag attctacaca gacttttcat tacaggatat tactcaagca
    38101 gatattcttc taattcccgg aggatttggt atagatgcgt tattgcacaa tacagccctt
    38161 atttcatgga taaaacaaat ggacaaaacc acactatgga ctacttctgt ttgttctgga
    38221 gtgttattgt tggccgaagc cggtatcatc aaagggaaaa ggtgcactac ccattggaga
    38281 cgtaaagaac aactgaaaaa ttacgatgtt gagtttgttg actcccgtta cgtacaagat
    38341 gggaaatata taacatcagc tggcgtttcg gcaggtatag atatggcctt atttctgaca
    38401 ggaaagatta tcggggataa aggagcaatg acgatccagc gaagtataga atacaatcca
    38461 atgcccccat acagctatga ttctattatt aaccaatcct aaaatatcgt actatggaaa
    38521 cttctgaaaa atttgaaccg atttgttgtc caaagttaga cccttctctt tgggaagata
    38581 aactgcttac ctggaatgat aaaaagtttg tcaaagccag tgtttttact atgttttata
    38641 tgcctgtgaa ttttggccag gtgatgcgaa agcttgacaa ggctgtttcg gccgccgggg
    38701 caactatccc cgattggctt tgcctctctg accacacttc aaagtggaac atggatgttt
    38761 atctggctgt agataaagaa attcctggtt tgcaaaatgt aacattatca ggcaattttt
    38821 tctgcaaagt atatgaaggg cattttagtg ataccaaaaa gtggtgcgat gattttcaga
    38881 aatcaaccga tactaagggc ttaaagattg acaaatggta catgtggtac accacttgcc
    38941 ctaaatgtgc caagaagtat ggaaaaaatt acgttgttat tgtggctaaa gtaaacagct
    39001 aatcgttggc gaaaacatta ttcgccaatg atttttacca aaaccctttt tcggcgtttg
    39061 ccgtcaaatt caccataaaa tatttgttcc catggcccca gatcgagctg gccattggtg
    39121 atggccacca ctacctctcg tcccataact gtgcgtttga ggtgagcatc ggcgttgtct
    39181 tcaaagccat tgtggcgata ttgcgaatag ggcttctcgg gagccagctt ctcaagccac
    39241 acctcaaagt cgtgatgaag gcccggctca tcatcgttga taaacacact ggcagtgata
    39301 tgcatggcat ttacaagtgc caacccttcc tttacctgac tttcggacag gcattgttct
    39361 atctgtcttg ttatatttat caactgcctt ctttgtggtg tttcaaacca aagttctttg
    39421 cggaatgatt tcatctttca aggttttaaa ataaagcctt ccggcgttta ttccggaagg
    39481 cttcaaattt aatacaattg ttttattgaa actttacttc tttattatca aaatacttgt
    39541 agtgccagaa ctactgcttg ccttaagtat atatgaacca gcaggcaaat ttgagccatt
    39601 atatgtaaca attttattgg ctgcagaaat cttattggcg acattatcaa ctcttctgcc
    39661 agacaagtca aacaaatcaa agctgtacgt gccgtcattt ataccttcca gcacaatatt
    39721 tatctggttg tcaaatggca taggataaac cttcaacccc atagtacttg ttgtttctat
    39781 ttcagaacct tttgtcacta ttacttcttc gcaatactgg gttgaatctc ccgttatcgg
    39841 gtcttctact gtatagcaga cattgtatgt cccaggagca gcatattcgt gagttggaga
    39901 ggttgtggtt gagtcggtct ggccatcgcc aaagtcccat tttagcttgg cctgatcgcc
    39961 caatcctgcc ccaataaagt caacagggta accacctgct ttaagggcaa tattttgtgg
    40021 tttggttata aataccactc tcattcctcc cggatttcct acatttatga gtttgtaagc
    40081 ttcatcagaa caggtagaag acagactcgt tacttttagc gataccagat aatcgcctgc
    40141 tttactatat gtgtttttcg gatttaacag tgtagaagag ttgccatctc caaaacccca
    40201 atagtatctg tcgggaatcc ctgatgaacg gttgataaac tgggtagtat aggtttcatt
    40261 atccacaatg taattgaaat tagcctcaca aatacttctc atcggaaatg aagatacttt
    40321 cttacacgca gtattggtaa tatttttatt gttgataaca gtcaaacata cattgaacaa
    40381 cccttcagtt ttatataaat gtgttgggtt ttcttttgta ctttttgtgt tatctccaaa
    40441 attccagata taccctttga ggtttccttt agaacgattg ataaacacca cgccggtatc
    40501 ggatggtaaa aaagtaaagt tagcaaagca gtcatcattg ctggctccgg cttgtatcac
    40561 ttcttttact ttatccgcac aaagggttac agggtttaat acggtgtgca atatagtata
    40621 atagcctttt gcaggaactg ttataatagg gttagcttca ttactaaagc tcccatcact
    40681 catttcccaa agatgcttta ataatggcct gttgtaattg ctataaagac ttaccgtatt
    40741 ttttgaggta tcggcaaaat agctaaacgc agcactgcaa ctgtctccaa ttgtaacaat
    40801 ctttgttacc atatctttgc agctcatatc tgcattggca gcaataagac ttactttgta
    40861 aacaccatta ttgacgtatg ttttaaccgg attgggttct tcggaataag tcccatcatc
    40921 aaatgcccaa aagtacttat taccttttga agtattggta aatgccactt gtttcagcag
    40981 cggttttacg gtaaatgtaa aagctgcttc acaactaacc gtccctacag taacacgttc
    41041 cgtgtatgtg tctaaacatt ctgtttcgct gttatatacg gtaaggctaa cattgtatgt
    41101 acctgtctta aacttgtgca ttggattttg catttgagtt accatattat cgccaaaatt
    41161 ccagtagtat atttctcctc ctgttgatgt attaacaaaa cgaacagtgt ctttgcttat
    41221 agtgtaagta aaaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacatt
    41341 tacagtccac accgcagaat catttgttgt tcggactgaa taataaacag gattattaaa
    41401 atcattttta gtaaaccctg cgtattgttg tatgccgttt acgtatgcgt tagcgccact
    41461 tgaaagcgca aaagatgcta tcaagtttcc aagagggacg tctttatcca caaaacaggt
    41521 tattttttta ttaacagagt ctatcttggt ggtacctact tggtttggca atgtaaaaga
    41581 taaaatactt atcccctctt tcttagtata attctttata taccaggtaa ctttgctact
    41641 gtccatggcc ctcacctcaa atgagtcggg gaaagaatat acataaatgc tatattcctg
    41701 cttactccct cctttccata tttcggcccc atcagatact ttaacataca acatattaac
    41761 attagtgttt gacgaaacat ttagccaaat ggtacggtta ggtttgtcaa gcatataatc
    41821 accatacata ccgttaaccg aaattacctc tttttccgac gaagcaggta tttctttgat
    41881 agtaaacgac ttgagctttt ccccaaaata gaagtagatt atttctcctt ttttgcaccg
    41941 tttggcatat ttactttcgg gtgatatgta atagtatttt gaagatgtgg acggacaacc
    42001 atttagttca tataaatagt tatatttcga atcgaccccg gaaagctcaa ctgcattatc
    42061 actaacacat ttataagcaa acacagcatt tatcggccaa acacccgaat taacacctgc
    42121 tgttgcaatt ataggttttg cgcatgaaac cccaagtgtt ggaataaccc gggttacttc
    42181 aatataatat ttattttcag taccatcttc agcaactacc cttacgctgt ctttggcaat
    42241 agaatcagaa tcattgtcaa aatacaggta catatacttt aaatcgtcag taccactaag
    42301 tttatctgat gaggcacgag gtgaaatgtt gcaccttgct gttagtgaaa catttttact
    42361 ccattctact tttgcagtaa tttttttatt agcattgtca atagtagcaa tgcctatttg
    42421 gacagattca atgaaaaatt gggtaaaaat ctttgctgta gaagcaaggc tgtattttct
    42481 gacttgcaat gtccatgact gagaattccc gactttgtca ataactgtaa tggtagtgtc
    42541 tgattctttt gtaaaagaaa tataacttcc cgatactaat gtttcattgg ccaatatcgc
    42601 ctttgaacca tttacaagac taaagtcaac tgttatcccg gaagagaaag attcatcgta
    42661 aggaaatgta gcttttacaa tgtggttatt gtaatcaata accatatctg catccttccc
    42721 attatagtca gccgatttga acgtgagaaa atcggtagta ttgtaaaatt tcgcaaacca
    42781 cgagaacgtg tcaactgggt tgtcccaatg cagataaacg ctgtcatctt tcttgacata
    42841 aatggcatca tttagggttt ctaaaggaaa atattcagaa gccgaagcat gtcttttatc
    42901 tgagtaaaaa gacactaagt taccttcagc ttttctgtat ggatgtatta tttgcaaaac
    42961 accattcttt tctgccttat atgcataatt gacacttggg ttgtctttga gttttgtcag
    43021 gttcaattta tttagtgtaa aaggggttat attatttgaa aacaaagaca aaggctttgt
    43081 tttttcagca ttttccaatg aaagcgcaac cttattatta tcaccaaagc atcctattgc
    43141 aaaaacatat gtattatttg caactgcatt aaaaacaaaa ggttcgccta ttgttggttc
    43201 ccaaacttct acatttgtaa ctacagacca gctccaaata ttcgaggttt caacgcttat
    43261 ttggtatttg ccgctaatcg ttggtgtaaa tgaaaaaaaa ctaaagaaat cactttcata
    43321 atagtttagc aaaggatata ctcctttccc gacatttatt gcctgtggcg aacgggcatc
    43381 cacaccattg gcaagacgtg ctttgttaaa agtcaaaaac aattgccatt gggggcttgt
    43441 attataatcg tccattattt taatataaat agcatcgcca gctttgccat ttactttgag
    43501 ctgactaaaa tcagcagaag ccgaataatt gaaactacca aggtttagca aatttgcatc
    43561 agaaaaattc tcattatcgt tccaaaaatc agaactatac gctggcttag ctttgtaagc
    43621 atatatgtat tgcgaagaac tgcgcactgt aattgacccg ttttccggga atacatattt
    43681 acaccattta gctttgtaat tacccgaaga aataatgttt ttgccttcat aaatattaaa
    43741 aggattttca aaactttctc ctttttccga atacatcgta tctacttcaa gtttccaaac
    43801 aatacgattc acataatcat tactaaaagc aaaatagaat gtatcttttt catttcctgt
    43861 tattatatta aacccatcag gcgtggtatt catacgttta acagttgtaa aaggcttaac
    43921 agaacgattt ttatatactg tcaaactaga gtaatatcca gattgtacat aatatcgcaa
    43981 agaataattg ttggccggaa ccggaatttt gtaaagagtt atgcctgtat ttgagaaaga
    44041 attctcacca acaatggcag ggatggcatc attaaaatca aaagacaggc tctgggcttg
    44101 catcttatat aaaaatacag tgcaaagcat catcaaaacg gctataattt ttttcataat
    44161 gaaatagatt aaatgttata ctatcaaaga tacaaaaaag aatacctaag tggttttata
    44221 gaaatgtttt ttttctgcca acattaaaaa aaaatattac ttttgcgcag ggtaagtctt
    44281 atacgaccag ctcccactga actcccccag gatcggaagg tagcaagggt aggtggttgt
    44341 agcggtgcga tataaggggc ttaccctttt ttttgtattt ttttgtgttt atttaaataa
    44401 ttttctactt ttgtacttat aaaaatcaga caatgaaatt aagtagtatt attctcgtgg
    44461 tcgttgtggt ccttttcgca aatcgaacaa ggtgaggata atattgttat aatatgaaca
    44521 aataaagaaa acccttctca ccgcaatgag aggggttttt tgttataaag aaattttaaa
    44581 attaaaattt agataaacaa atgaaacacc catgattctg caaatcgcaa acacgatgat
    44641 attttattgt gggtgttaca tcattccatt aaaattaaaa aataaatatg atgaaaaaca
    44701 aattacgcag tcacacaacc acacaaggcc gattaatggc aggggcacgc agtttatgga
    44761 gagcaaacgg aatgaccgag caacaaatgg gcaagccggt tattgccatt gttaattcct
    44821 ttacacaatt tgttccaggc cacgttcacc tgcacgaaat tggccaggaa ataaaaaaaa
    44881 tgatagaagc agaaggattc tttgctgcag agtttaacac tattgccatt gatgatggta
    44941 tagccatggg gcacgacggg atgttatact cgctgccttc gcgcgacctt atagccgata
    45001 gtgttgaata catggttaat gcccacaaag ttgacgctat tttgtgtatc agcaactgcg
    45061 acaaaatcac cccgggtatg ctaatggctg ccatgcgttt aaatatcccg gcagtttttg
    45121 tatcaggcgg gccaatggaa gcaggcgttg ttcgtgacaa aaagatggac ctcgtagatg
    45181 ccatggttat gggagctgat gataccatag ccgatgacta tctggatgaa gttgaaaaga
    45241 atgcttgtcc tacctgtggt tcatgctctg gtatgtttac cgccaattca atgaactgcc
    45301 tcaacgaagc tataggcatg ggacttgcag gcaatggcac tatcgttgct acccataaaa
    45361 accgcacaaa actttttgaa aaagctgcca aacgtattgt agaaataacc aaagcatact
    45421 attatgacgg ggacgattct gtccttcccc gcaatatagc taccaaagct gcattcaaca
    45481 atgccatggc ggtagatatt gccatgggag gttctacaaa cactgtactt cacctgttag
    45541 ctattgcaaa ggaagcagaa gttgactttt ctatgaaaga catagatgcc ctttcacgta
    45601 aagtaccagt attatgtaaa gtagccccaa gctctcatta tcaccttgaa gatgtaaacc
    45661 gtgccggcgg gatactcggt atcatggccg aactaagccg tgctaacctt attgacacta
    45721 cggttaaacg cattgacggg cttacattag ctcaagctat tgataaatat gacattttga
    45781 gtaaaaacgt aactgaagaa gcattagata tttataaaag tggccctgca ggcatccgca
    45841 acctgaccat gggatcacaa ggatatatgt ataaagaact tgataaagac cgtactcagg
    45901 gttgcatcag ggatatagct catgcttatt tccccgatgg cggactggct gttttatacg
    45961 gcaatattgc agaaaaaggt tgtattgtaa aaacagccgg agttgaccct tccatcttca
    46021 aattcagtgg aaaagcaaga gtttttgaaa gtcaggaaga tgcttgtgat ggcattttaa
    46081 aagatgttca ggccggcgat gtggtgatta tccgttacga agggcctaaa ggaggcccgg
    46141 gtatgcagga aatgctttac ccaacatctt atatcaaatc tcgccacctt gggaaggaat
    46201 gtgctttgat taccgatggc cgtttttcag gcgggacatc aggcctgtcg ataggccacg
    46261 tttctcccga agcagctgca ggcggtgcaa ttgcgcttct tcgtgatggg gacaaaattg
    46321 aaattgacat tcctgcaaga aaaatcaatg tagtagtttc agaagaagaa ttgaaatcac
    46381 gccgtgccca ggaagaaaaa cgtgggaaag aagcttatac tcctgcaaaa cgcaaacgca
    46441 atatttctaa agcattgaaa gtatatgcca gcatggtttc aagtgccgac accggcgctt
    46501 tcaggattat tgaataagta ataaagttta aaggttttga aaatatgaat aaagaaataa
    46561 aaaagataac tggttctgaa gcgatagtat tgtcgttatt agaagaaggt accgaaatta
    46621 tgtttggcta ccccggcggg gctattatgc cggtttatga tgccatgtat aactatcagg
    46681 acaagctaca tcacgttttg acccgccacg aacaaggggc tattcatgca gcccaaggct
    46741 atgcaagagt taccggtaaa gttggagtat gcatggctac ttccggcccg ggggctacca
    46801 atcttgttac cggtctagct gatgccatga tagacagtac cccaataatt gctatcactg
    46861 gtcaggttgg agcacacctg cttgggactg atgcattcca ggaaacagat gtcgtaggta
    46921 tctctatgcc agttactaaa tggaacattc aggttactaa gcctgaagat attgccccgg
    46981 ctattgcaaa agctttttat atagcacgta ccggtcgtcc agggcctgta cttgttgata
    47041 taactaagaa tgcgcaattt ggaatgtgtg agttttccta tgaaaaatgt aactatatac
    47101 gcagttacgt ccctcgtcct actattgacg aacataagtt gaccgaagct gccaaactta
    47161 tcaatgcggc tcaaaaacca atggtattag ttggacaagg agtaattttg ggtaacgccg
    47221 aagatgattt taaagctttt atacaaaaat caggtattcc atttgcctgg actatattgg
    47281 gggcaagtgt aatgccggtt gaccatcctc tcaatgtagg aatgcttggt atgcatggca
    47341 attacggccc caatattaag caaaacgagt gcgatttgct tattgctatt ggcatgcgct
    47401 ttgacgaccg ggttaccggg gatcttagcc gatatgccaa gcaggccaaa gttatccaca
    47461 tggatataga cccttctgaa ataaacaaga atgtaaaggc tacggtagct atcctgggca
    47521 atgtaaaaga aaccttgcca ttattgaccc gtctaattga gaaaaaagag cacacttcat
    47581 ggcgagcaga atttgatgca tgcatgaaag ttgaacagga aaaagtcatc aagcatgatt
    47641 tatttcccga aaaacaagga ttgactatgg gcgaagtttt gcgccgcgtt aatgagcgta
    47701 ccgatggtga tgctattctg gttacagatg taggccaaca tcaaatgatt ggttcgagat
    47761 attttaagta taaaaataaa agaagcttca taacttcagg cggattaggc actatgggct
    47821 ttggtttgcc agcagcaatg ggagctaaaa tgggtgctcc cgacaaacaa gttatactaa
    47881 tggtaggtga tggcggcatc caaatgacca ttcaggagct gggcacaatc aagcaaacgg
    47941 aaattcccgt taagattatc ttactgaaca acaacttcct tggtatggtg cgccaatggc
    48001 aggaattgtt ttttgacaaa cgctattcaa tgaccaagct tatcaatccc gattttcaaa
    48061 aaattgcaga agcttatgac ctgaaccata gcaaggttac atctcgagac ctcttagata
    48121 aagccattga agagatgtta gcttcagaca aagcatattt gcttgaagtt gttgttgaaa
    48181 aagaagacaa cgtatttcca atggtcccga caggggcatc ggtaagtgaa gtactactcg
    48241 aagctgcaaa gtaatattaa tagtgttaat caaactttcg attgaagtaa tcggacgaag
    48301 gataaataaa aaaaacatga atacagtaga acaatatacc atcattgctt tttcggaaaa
    48361 tgccattggt ttgctaaacc gcataaccat cattttcacg agaaggaacg taaatatcga
    48421 gagcctcacg gtttctgagt cttctctaca tgggatccac aaatttacca tagtggtaaa
    48481 taccactgcg gcccaggttg agaaagtggt aaagcaaata gagaaacagg tagaagtatt
    48541 aaaagctttc ttttttacaa agaatgaaat tattttccag gaaatagctt tgtataaagt
    48601 gccaacagaa gagttgttgc gtagcaatat gatagaaact attgtccgcg aaaataatgc
    48661 ccgcattatg gaagtcacgc aagactatac cgttattgaa aaaacgggcc acgaatacga
    48721 aaccagcaag ctctttgccg agctcaataa attcggggta aaacagttta cacgttcggg
    48781 ccgaattgcc gtaacccgct ctaatgttga gcttcttaca gcatttctta atgaacgcga
    48841 gagcagcagg ctttcttaat tgaaagtaac aagtttttac atagccaaag ccttccaggt
    48901 tttaaaaacc tggaaggcct gatattgttg ttttaattaa tcaaatatga aaatctccaa
    48961 ttatattgac gtattaaaca ttcctttttc tgtaaaactg cctaatgata agtctcttga
    49021 acgttcagtg caaatgtata tcttggtagg agagcaattg gctcttatcg atacaggagt
    49081 agcaggcagt gaaaaggtta tatttgacta catcgaatca attggcagaa aacctaaaga
    49141 aataaagcat atcctgctta cccataccca tcctgaccat attggggctg ttagtgctat
    49201 taaagaagct acaggggcta aggtatatgt gcatgctgcc gaacaggcat ggctggaaga
    49261 tactgaaaaa caatatgccg aacgcccggt tggcggcttt catgagttag ttggaggatc
    49321 ttgcaaagca gatcaggtac tgcacgatgg agaagtgcta catttatgtc ataatctgac
    49381 cttaaaagcg atacatagcc ctggacattc tgcgggttct gtttcgtatt tgttgcaaca
    49441 ggaaaatgcc ttgtttaccg gcgatgctat tcctgttaaa ggggatatcc caatttatga
    49501 tgattgggct caaagcttgg ccagtcttga aaaacttgag aacctgcctt tccctgcatt
    49561 tgttttgcca gcatgggata gccctaagac tggcaaagat gctgaagagg ttttcagtca
    49621 tggttttgat atgctgtatg atgtacatca agctttttgc gaagctaaag atgaggttgg
    49681 ccttgctgat atgcaggctg ttgcggcaag catgataaca aagttacaac tgccagccca
    49741 gtcaatgaac cctcttttta tcaggtcagt gatgagccat ttgaaggtaa ggtaggttat
    49801 tattgaagta gaagctattc cacactctta ttgttattgg catacccccg ccatttttca
    49861 ataacttgct gcatatcagc agggagaggt gactcaaact gcaccctttt gcccgttgca
    49921 gggtggtcaa atgccaaaga gcgggcatgc agggcttgac gaggaagaat gtcaaaacag
    49981 ttttccacaa actgcttgta ttttgaaaaa gaagggcctt taagtatagt attgccacca
    50041 tattcagcat cgctaaacaa tgggtgccca atgtgtttca tgtgtacacg tatctggtgt
    50101 gtacggcctg tttcgagtac gcaatctaca agtgtagtca tcccgaaacg ctctattact
    50161 ttgtaatgcg tgatggcatg tttgcctatc tcagggtcat cgtaaacagc cattactttg
    50221 cggtctttgg cgctgcgcca tatatttccg gtgatggtcc cgctatcttt agtaagattt
    50281 ccccaaacca aagccgtgta atgcctttca atggtgtggt agaagaactg gctggccaat
    50341 ttgttcagtg ctagttcggt tttggcaata accaatattc ccgaagtgtt tttgtcaatg
    50401 cggtgtacca gtccggggcg catctcgcca ctttgaaaca taggcaaatc cttgaatttg
    50461 tagtacaacg cattgaccaa tgtcccggta aagttaccat aagccgggtg tacaaccatt
    50521 ccggcctctt tgtcaacaat tacaaggtgc tcatcctcat atactatatt caggggaata
    50581 ttctcgggga ttatctctgt atcgcgtggt ggaaaggtaa gcacgacaga tattacatcc
    50641 agcggcttta ctttatagtt tgactttaca ggcttattat tgaccaggat atttccggct
    50701 tcagctgcat tctgcacttt agtgcgcgaa gcattaacca gcctgttaaa gatgaactta
    50761 tcaattcgca ttggcgcctg tcccttgtcc acagtccagc gatgatgttc aaataattcg
    50821 ccagtttcgt tattttctgc tacttcgttc aattacttct tgataaaacg tgaaacacta
    50881 acattgttgt caggtgcgat tagcttaata atgtacatcc ctgattttag attgccaaca
    50941 taaatagatg atgcgctttg catagataat acaaggcaac ctgttaaaga ataaatctga
    51001 accttgcaat tattgttggt tgagcaggtt aggtttatat aattcccggc agggttcgga
    51061 tataccgaaa actcagcagg ttttgtagtg ctttttaatg aaacaatggt agggtcgcca
    51121 aaaacagggc gcatcataag agccccagtt ttactaactt ttgatttagc ccaacccgat
    51181 gctatccccg taaaattaac aaaatgaaaa gcattagcag tatcattcct gtcaaaacca
    51241 acattaagaa aggcttcatt cttttgttgc cagccaatat aaaacacggt atctactaat
    51301 attcttttac ttaaaatata acgtttaaat tgttgagggc cctttgcaat ctcaacaact
    51361 ttacgttcgg cataaattga gtcgcccggt ttacctttgt tgtcggccca aacaaataaa
    51421 ttaaactctg tttcattctc ttcattcaaa gttgggttaa aataaatatc aatagcagaa
    51481 agggtatctg gcttataggt tgagtatttc aatgcaacca tggctgcaga gatattggcg
    51541 ccctttaaac cataacctaa ttcggcactt ccatcgtcat aagcatagta gttggcaaat
    51601 ctttggtacc atttagagct gttgttaaat gaaatgctgt ctgcttctga attttcaatt
    51661 tttgcctcaa tctcaaaaag cgtagattcg gtttcgtctg aagttaagtt gatggtttca
    51721 aactgatagt cagtatattt gtgaggaggg caaactatca tcgtagagtt tttctcgtaa
    51781 ggataaactt cgttgccaat aatttcttta aattggaggc ttacctgtac gttagcttcg
    51841 taactcccga agttgtatat cttttgatta aataacggtt tggttgtctt gtaaaaaaag
    51901 tccctgtaat gtttccaggg tatagaagtg tatttgagaa gtattgatcc ggcattctgg
    51961 gcaatggcat tgtcaataaa agcggtatct gccattgttc tgttttttat aatacagacg
    52021 taatcaatat tccagaaatc gccattagaa gctaaaccgg gattttggtt ttcgtttttc
    52081 agactgactt tattgataaa cctgaaataa aagctatctg tataaaaagc ttcatctttt
    52141 aaggccattt taactatctg gaaattagtc aatacatctt tgtttgggcc ttcttttttc
    52201 caaacaacag taccattgtc cgaactattt ttataaaatt tgagtatcag gctatcacta
    52261 gtttcgggca tatctcccag tccgccaggc tggtaaaaaa aataaaagta caagctgtca
    52321 tctggtgtat atcttgataa atcaatgtat tttgaggtta acgtgtcagc attgctcagc
    52381 tcatcagatt cagcagactt gtacaattca cctttactgt tcaagccgtc aaatgtagca
    52441 acgccaattg tatatggttt gtagccgaat gaacggttta tgcaaacgtt tttgtccctc
    52501 cagtttgtcc caagcgggta acaaaaggcc gagtcgttgg cataccaaaa gtcgtcgaaa
    52561 aaaacaatct gcttatccgc tttttgagtt gcagtggctt ttaaagaagc ttttgttgtt
    52621 acaggagatg ctttaactgt ataagttaca ggggctaaaa attcttcttg tccataagtt
    52681 ttttctgttt taaaaaagaa gaaacttgcc gtaattaatg tgatgaagat atatttacta
    52741 ttggccatta aggtctcttt ttacggttaa ccacaattct acactattcc ccgaagtgct
    52801 gtatgtactg tctttaggcg cttgcttgta aactacagct ttaagtgaat ctttccaatt
    52861 ctgaacagta ctatcaaaat gaaatttgtt aacattgaga tatgaaagta tagacttttt
    52921 gcgggcttgc tgaagagtta accctttaag ggaaggcacc atagtactct ggtcagaact
    52981 ttttcctaaa attagtttga cagatgagcc cttgtatatt tcttttcccg gggctatgat
    53041 tttgccttta aaaacctgct ttaacactaa gtttgccgct aaatctgact cgtaattgat
    53101 actctgtact tgtaaccctt gcgattcaag tattgccacc gcctgacgaa gcgaaacacc
    53161 aacaacatca ggcattttca ccttttcagg gtttatagca ttcatgatca aaaatatccg
    53221 cctgttcttt tttacatttg ccccttttct gggatattgg tctaacactg tgccccgctt
    53281 actttcaata ttaaaaacag agtcagaaat tgctatctgc aggccataat catcggccag
    53341 ctcttcggcc tgctgtaaat caaggccaga aaaatcgggc acagttaaat atttattatg
    53401 ccgtgtagca cagtgcaaaa caatactgga agcaaataca atggcaaaaa atacagctgc
    53461 catcactttt acctgttcta aaaaaggttt gctttttaaa aattcaacaa cagactttct
    53521 tcccatgagc aagtttttca atagtaaacg taaatatttg caaaaatata ttttttgcca
    53581 tgcgtaatat cgaaaaaaac aattctttta ggtattgcct aattctcaaa agtttgaata
    53641 acattataaa ccgaatcgcg ctcggtgggg gtataaccgg cagattttat cagcttcagc
    53701 atttcatcca aggtcatagt gtttttttct ttaaagccag ccatggtgta tatttttgta
    53761 gtgtcatcta tggtgccatc aatatcatca actccaaatg agagggagat ttttgtggtg
    53821 tcgcggccta tcatgggcca ataagcctta atgtgcggga tattgtccag aaacaacctt
    53881 gaaatggcat agttacgaat atcttccacc accgaaactt ctccaatatg agaaagatgg
    53941 ttgttttctt ttctgtattt cagtggaata aaagcattga agcctttcgt ttcgtcctgc
    54001 aactgtctaa tcctcgacat gtgggcgatg cggtgttccc acttttcaat atggccataa
    54061 agcatggttg cattagacgg aatgccagca cgatgagctt cgcggtggat ttccagccat
    54121 ttttcgcctg tcgatttctc cgggcacaat attttcctga cttctgcatc aaatatctct
    54181 gctcctccac ccgggatgct gtccaacccg tattctttta atgcatgaaa gccttctgat
    54241 aaagacattt tggcctgctt aatcatgaaa tcaagttcta tagccgtaaa agcttttaca
    54301 tgaatagccg gcatgatgct ttttataagc tttatcatgt ttccataata atgcaaatcc
    54361 ctttggggat gcacgccacc tacaatatgg atttctgtaa cagaggtatt tttgtatgct
    54421 tctacaatcc ttgcaatatc gtcatatgta tattcccaac tcccctcttc gcctattttt
    54481 cgcttgtatg aacaaaactt acattgataa agacaaatat ttgttggctc aaggtgaaaa
    54541 tttctgttga aaaatacaag tttttgactt gcctgctgct tggcatgact tgccaaaacg
    54601 cccaaccatg cagtatctgc ctgggtatat aacaataatg cctcttcttc actaatgcgc
    54661 cggtggttgt atattttatc ggcaataacc tgtaaggctt tatttttatg atgtaaaaag
    54721 ctttccatta aaaatatctt tttgcaaagg taaacaaaca aaaaaaggct gtccgaaatg
    54781 gaacagcctt tttatagttt tgttcagaaa cttatgcttt gtgtttatgt tcgtcagaaa
    54841 cagtaagttt tttgcggcct ttagccctac gagctgccaa tactttgcgt ccgttagctg
    54901 ttgacattct ttcaagaaaa ccatgtttgt tttttctctt tctgtttgat ggttgataag
    54961 tacgtttcat acgtatatta ttttatctgt tattattctt tttgttaagg actgcaaaag
    55021 taatgatttt ttgaaaaatg caaaattttt cctgaaaatc attttgaaaa atatgatatt
    55081 gtccccacaa ttttacattg cccgtatcaa aaaaaaatgt atgtttgtgg tcgcttgcaa
    55141 taaaaattcg agtattaaac aattgcaaaa tttgataatc attaattaaa aatcagatga
    55201 aaaatatttc tttaattgtg aatattgtac tggctgtagc gctgggtgtt ctttttgttt
    55261 tgcatttttc tttgcgtagc aaagttaatg aagctgtatc tcacaaagtt gttgcttctg
    55321 gcaattctaa cattgtcttt gttaacatgg attctttgta tgcgaaatac aacgaatacg
    55381 cagacttaaa agagcaacta caaaaaaagc aaagcaaaat ggaagcagat ataaacaaca
    55441 gaaaaattgc ttacgaaaga aaagttgctg attaccagga taaagcccaa aaaggattat
    55501 tacttacctc tgaacgccaa cgtatagaac agcaactgat gggcgaacaa caagagttga
    55561 tgcaatatgg ccagaagctt caaaatgaat tagctgaaga atcaagagtt ttgaataata
    55621 aactaggaaa caatattatc gaatttttga aagagtataa taaagatggc cgttttcaat
    55681 ttgtactgag ccatgtttat ggaggaaacc ttttgtatgt aaatgactca ctggacatta
    55741 ccaaagatgt tatcgaaggg ttgaacaaaa aatacgataa agagaaaaaa taaattattc
    55801 aatgggtttt gcatctggct attttgaaag gtatcaaacc agagagcctt taattaacga
    55861 agctccagac tccaatcttt cgcttgtcgt ttgcattcct tcatataacg aaaacaatat
    55921 aataagggcc atcgaggccc tttattgttg tacccccttg caaaacaaag ccaaggtaat
    55981 aatcttgctg aattataccc aaaataatga gccaatgaaa gcttttcacg aacggcaact
    56041 cattgaaacc caacaatgga taagcaagca tcaacgggct gatatacagt tccatgtttt
    56101 tctgaaagct ttaccagcca aagatgcagg agttggcctt gccagaaaga ttgcaatgga
    56161 tgaggctttg cgattgttta atttgctcga caaacgcaat ggtatcattg tgggatatga
    56221 tgcagattgc acctgtaaac ctaattacct tgctgaaata gaatcctttt ttataaaaaa
    56281 cccccgagct cccggcattt ctgtttattt tgagcaccct acagaaggag aagagtttga
    56341 acctcagata tataaagcta ttttacaata tgaactttat cttaggtatt atattgaggc
    56401 gttacgatat tgtggttttc cgtatgcttt tcaaacattg ggttcttcat ttgcagtacg
    56461 agcagatgca tatgcttcgc aaggtgggat gaacaagcga aaggcaggcg aggattttta
    56521 ctttcttcac aaaatcatac ccctcgggag atatgatgaa ttaaacacta cagccgtata
    56581 tccttctcca cgcatatccg acagggtgcc ttttggtacc ggagcagctg tgtctgatat
    56641 tatttcggat actgaaggat acaaagtgtt caaccctaaa tcgtttgagg ttttgaaagg
    56701 cttcttttct ttagtgccaa agttttataa agcagagaac gcttttattc ataaatatat
    56761 tgaaaccatt ggaccttcat tcgcagagtt tttgaaatca gagaaaatgg ttgaggctgt
    56821 tgccaactgc aatgctaatt cgtccaatat caatgtattt atacgccgtt tttatgcctg
    56881 gttcaatgga ttgctggcat tgcaatgtct caataaattg catgaatcag cttttcaata
    56941 tgtcccggtt gctgaagcgt cgaatggact gttgtctatg ctggacaaac aatatgaaca
    57001 agcaaactac agcgagaatg agcttttaaa aatattcaga caaagagttt ttaagttcaa
    57061 ggtttcgtgg aaagaaaatt atagttttga attgatagag taaataaata tgccctacga
    57121 actgttttct gacgaatatt acatgaacga agccctgaaa gaggcgaaaa aggcttttga
    57181 caaagacgag gtgcctgtag gggctgtaat agtagctaaa aaccatatca tagccagagc
    57241 gcacaacctc acagaaacgc tgaacgatgt cacagcccat gccgaaatgc aggttattac
    57301 tgcggctgca tcggcaatag gaggaaaata ccttacagat tgcactttat atgttactgt
    57361 tgagccatgc tttatgtgtg ccggggcgtt gtcttgggct cagataactc gtgttgtcta
    57421 tggtgcaaca gaccccaaaa aaggctttat gcgcagcgat tacccgcttc tccataagaa
    57481 aacccaagta accgctaatg tcttaaggga cgaatgtgct tcactgatga agcaattttt
    57541 tcaacaaaaa cggtaattaa ttagcaaatt cggtaattag ctaatgcttt tctgtatttc
    57601 atcagctaat tttcaaattc tcgaattttc aaatttactt tccaggctgt aaagttgcat
    57661 aattgccaga aaatgttact tttgcatagg gtcggttatg aaacataaag acctttttga
    57721 gtattaataa tgatgcggaa tttgatatgg tagttttatg aaggttcgat tttgacaata
    57781 gttttgatag atagcaagct aaaactattg tctttgaggg ataacatgcg catcaggttt
    57841 catggccgcc cggattttta tccaatttgt cagatataat ttttatatag gctctttgag
    57901 tatttaacta aaaacactga cacatgaaaa gctatgttac aaatcaaatc aggaacattg
    57961 cactcttggg cagctccgga tcgggtaaaa ccactcttgc cgaagctatg ctttttgaga
    58021 gtggcgttat agaacgtaaa ggttctgtag agcaaaaaaa taccgtttcg gactataacg
    58081 agatcgaaca caacaacaag gcatctgttt tttcatcagt gatgtatgcc gaagtcaacg
    58141 accgaaagct taatatcctt gacaatcccg gaatggacga ttttataggg gctaccctta
    58201 cttctttaca tgtagccgac agtgctgttg tagttatcaa tgcccataac ggtgttgaag
    58261 ttgggacaga aatacacgca cgtcatctca aaaggctcaa taagccaatg atacttcttt
    58321 ttaatcaaat ggaccatgaa aaggccagct atgagaaaac attagaagca gcccgcgaac
    58381 gtttaggcaa aaatattacc gttgtacaat acccggttaa tgaaggtaca gcatttgata
    58441 ccattatcga tgtgatattg atgaaaatgc tcaaatatcc taaagaagga gggaagcctg
    58501 aaattacaga aatacctgca gcagaagcag caagggctgc cgagtatcat aatgcgttag
    58561 ttgagaaggc tgctgaaaac gatgataagc taatggaact ttttttcgag aaaggtacat
    58621 tagaagtaga agatttacgc agcgggttgc gtcttggggt gttaaaaagg gatgtattcc
    58681 cggtttttgt ttgttcggca aagaaaagta ttggcattgg gcgttttatg gagtttttgg
    58741 gcaatgtagc cccttctcct gaaaaagttg aagggcttaa aactactaat ggcaatgaaa
    58801 taaaagtttc tccagatgga gctccttcat tatttgtatt taaaacatct atagagcctc
    58861 acattggcga agtatcatat tttaaagtga taacaggcaa agttactgaa gcaatagatt
    58921 taaacaacaa taataaccaa ggcaaggaac gccttaccca attgtatgta tgctctggca
    58981 agaaccgtac aaaagttaca gaacttatag ccggggatat tggcgcaact gtaaaattga
    59041 aaaataccaa aacaggacac accctggcgg gtggaagttt ttctgaaatg ttcgaaaaaa
    59101 caaccttccc tgagcctaaa tttaggattg ccatcaaggc aaaggttgaa gctgaagaag
    59161 aaaaattggg cgaaatactc atcaaactcc gaatggaaga cccgaccatt ttgatagaac
    59221 attctaaaga attgaagcaa acaatcatct ccgggcaggg agaataccat ataagcacct
    59281 taaagtggca tctcgaccat attcacaaaa ttgacgtaga gtttatgtct cctaagatcc
    59341 cttatcgtga aacgataacc aaagtggcag ctgccgatta caggcacaaa aaacaatcag
    59401 ggggggccgg acagtttggt gaagtgcacc tggttattga accttatgta gaaggagcac
    59461 cagatccggt tgtatataag gttgctggca aagaaatcaa acttaatatc cgagataaac
    59521 aggaggtggc tttagcttgg gggggcaaac ttgtattcta taactgtatt gtaggtggtg
    59581 ctatcgatgc ccgctttttg ccggccatta tgaaagggat tatggaaaag atggaagaag
    59641 gcccgcttac aggttcgtat gcccgcgata tccgcgtagc tgtttacgat ggaaaaatgc
    59701 acccagttga ctctaacgaa atctcgttta agttggcagg gcgtaatgca ttcaaagaag
    59761 catttaaaaa tgccgggccg aaaattttag agcctgtgta tgatgttgaa gtacttgtac
    59821 catcagagcg tatgggagat gtaatgagcg atttacaaac ccgacgtgca gtagtgcttg
    59881 gcatgtcaag tgatagcgga tttgaaaaaa tttcagcccg tgtacctctt gccgaaatgg
    59941 ataaatactc tacatcatta agctcattaa cccagggaag ggctatttac aacatgaagt
    60001 ttgccgaata cgccccaaca cccgccgaag ttcaaaacga actgctcaaa gcctacgagg
    60061 ctgctgaagt agaagagtaa tatttatttt tatcctttca cataaaaaaa ccttctgata
    60121 tgtttaacag aaggtttttt tatttacacc caatttaacc ataaggtggt ttttcaaagc
    60181 cgaactatat gatttagttg ggttaaataa aaaacacctt catctgcgtt tttgacctgt
    60241 tcaccgaaaa atatttttta ttgggggtat aagctgttta atttgtctta aaataaactt
    60301 taaaaaaatt aggccatcgg aattcatctt tgtattcatt acagaaacat gattgaatca
    60361 gatgtttcag ttttaaacag tttaaattgt aagagatgga taatacaaca atcaaccaac
    60421 cggtaactta ttcattaagt ataaaaggag acgaaaaaag tttttacaaa gaattggaga
    60481 tatttaccca atctgttatt caggagctga gcattttttt caacaaagaa gtagatggcc
    60541 ttgtaaagtt cctttcgcag aacaaaactg aagagctcag gacaaaagag gaatactctt
    60601 ttgaattgat tatcataggc actttgtgga atatatataa tggatatgcc caaaaagaca
    60661 atagctttgt agttgacacc ctgctgccgc atctaaataa cctgcgaaaa aagaaccaga
    60721 tgcttaagcc ttacgtggac gatgtaaaag gctatttttt atcccgcata tattctaatc
    60781 ctgaagttga tgttaaacct gagtattctt tgtatgagtt taaaaggctt atcaaatggc
    60841 ttgatgctac aggcgaattt ggcgaagaag taaaacggat gaataactgg tatgcttttt
    60901 tactgggatt ttcagacgag agagtaaaac aattgttatc aaaagtatca tcttatgcaa
    60961 cttatttctc agatttgtca cggaatgcat taggacaata tactaatggc gttcacaatt
    61021 atttgaaaaa tatacatcca cagatgaaaa accgtgagga ttatatattc tgtggcagaa
    61081 aagaagaaga atatctgatg aaccttgtgg gtgctgaggt tctaaataag tcattaaaaa
    61141 aatcctttag ccaggcctca aaacgggtaa tattattgcc tacctgcatg tgcaagccgg
    61201 ataatgggca atgtaaagct atttttgctg agatagaggg caaatgtaca agttgtagca
    61261 ggaactgtat tgtgaatact ataaaaaatc aggttgaaga taaaaatacc acagtagcac
    61321 ttatcacaca ctcttcatca tttagcagag tgttgcaaaa atatgaaaat caaacaggcg
    61381 taggattggt tggtatcgct tgtattttga acctgttgac agggggttat gagcttaaaa
    61441 ggctcaatat ccctgctcaa tgcgtttttt tgaattatag cgggtgtaag aaacactgga
    61501 ctaaagatgg cgtccctact acgttgtata ttgaacagct caagcaaatc ctcgcacaaa
    61561 aagaaattgc aacaactcat gaggctaaag aagaggtaaa aatggctgtt taattatttt
    61621 ttaaatttca caatgtgatt tcgcctctta atgaggtttc aagcccccgg acaatatctt
    61681 cccgtttttc atagcgccct aaaaggtaca tcagcttagt aattgcagct tcagttgtca
    61741 tatcgtgacc actaattaca ccacactctt ttaatgcaag acttgtacgg tacatgccca
    61801 taaccacgct gcctccaaaa cattgtgaca cgttcaatac tatcaatccg ttgctaatag
    61861 cgtatttcaa cagggaaata aaccattcgt cggtaggcgc attgcctgag ccaaatgttt
    61921 cgagaacaat agcctccaaa tcggggcttg taagaactga cgacacaaat gacgggttta
    61981 ttccggggaa caattttaaa aggcctattt tgccattgag cttagtgctc agtttaagtt
    62041 ctcgctccac tgtaggataa ttgatagatg ggaagttgta tttgatatga atgccaattt
    62101 ctgcaagagc agggtaatta accgaccgaa atgcattgaa atgctctgca ctgtattttg
    62161 tggcacggtt gcccctaaaa agcttattct caaagtaaat acaaacttcc ggaacgacag
    62221 ctaagttgtt tttcttttct gcagcaatct ctatggctgt aataagattt tctctaccgt
    62281 cagtgcggac tgcacccata ggaagctgcg ccccagtaaa tataataggc ttttgaaggt
    62341 tctcaaacat aaaactaagc gctgacgcag tatatgccat agtatcagtt ccatgaagca
    62401 ccacaaaccc gtcatacaga ttgtagcttt cttctataat ttttgccagt ttaacccaaa
    62461 tatcgggcgt cattgtagcc gaatctattg gagggctgaa agtgaccact ttgcactcag
    62521 tctgtagttt gctaatttcg ggcatctgac gcaaaagagc ttctaaatca atcggaatta
    62581 atgaattatt atcctcatca tgcttcatgc cgatggtccc accagtataa ataatcaata
    62641 tggaagaagt gttgctcaaa gctcctgaaa ttacgataaa ggtagaaaat gaaaatggca
    62701 attcaaagaa agcgtggcct tatattatcc tgtaaatttc cataatacct ttcaatatgt
    62761 tgtcaaaatc aatttgcaag tcaataagct taccactgtg aatgtcaaac acccagccat
    62821 gaactgtgat atgcctttct ttatatgctt tttgaacatc tgaagttttt aggatattca
    62881 cgcattgttc ctgtacatta agctctacaa gacgtttgta tttttcttcg gtatcggcaa
    62941 tggaatctaa ttcatctttg tgcaagcgat acacatcgcg gatattacgc agccaggggt
    63001 ttaatattcc aaggtcagca gactgcatgg ccgactttac accggtacaa taatagtgcc
    63061 cacaaactac aatatgatta actttcagat gactaactgc ataagtaata acagacatca
    63121 cgcttagatc ggtattgggt accatgttgg caatgttccg atggacaaat acttctccgg
    63181 gttttgcccc catcagttct tcggctgtaa ctcggctgtc tgagcatcca atatacaata
    63241 cctcagggtt ttgccctttt gaaaggtttt tgaaatattc cggatcaact tttagctttt
    63301 cggcaatcca gctctcgttg tttctgaata tttgagctat gttcataacg tttttatttt
    63361 ttaaacaaga atttggatat taatgttcta tgtgcataca gtattcaact tctcaagata
    63421 aataaaagtc aaatcatcat tgatttttac ttgtttaaac tctttgtatc ccattttatt
    63481 atagatgtat aagtttttgt cgtcacggaa gccggtaaag agttcatatc tgtttactgc
    63541 aggaaactgg ttttcgatag cactcatcaa ttgcctgcct atgcctttgt tttgaaattg
    63601 gggatgtaca accagccgtc caataaagca tgtgtcattc ttcatatttc cccggacact
    63661 tccaataata tgcgaatctt caacagcttt caggataata ctatacctga aatcgttctt
    63721 tagctcttcg atggtttcca tcaaaggggg gatgtgaaaa tcattatatc ttacggcatt
    63781 ttcgtgatag gataactttt gtaggtcaag tatttcgtgc aaatcgtcta tttgggcttc
    63841 tttaatttgc ataatgaaag atgtttataa attaaaaaca ctctttgcag ccaatgtagt
    63901 tacttcggta acttgttctt tctctacctg atatatttcg gatagtttgc tcacaataag
    63961 ttgcaaatag gctggttcat ttctctttcc ccgatacggt gttggggtta aatatggcgc
    64021 atcggtttcg agtaccaggt ttttcaggtc tattgaacca atgattttgt ccaggcctcc
    64081 atttttaaaa gtaaccacgc cgccaatccc taaaacataa cccaaatcaa tagcacgttt
    64141 ggcttgttct acagtgccag aaaaagcatg aaacacgcct tttacatcag ggtgcttttg
    64201 cacaatatcg agagcttgct ccactgcgtc ccgggtgtgg atgataacag gcagatgaac
    64261 ctcttttgcc cattgcaact gtatttcaaa tgcatcaatt tgttctttgt aaaatgtttt
    64321 gtcccaatac aagtcaagcc caatctcacc aatagcaata ggtttattat ttgaaatctc
    64381 tttttctatt atagataaaa gggctttgta gttttctttt accgaacaag gatgaatacc
    64441 ataagctggc ttgcatatat caggaaaggc tgaacaaatt ttattcagag cttctacagt
    64501 tgtttcgtcc acattgggat tgatgatggt agaaaccccc tgatatacac ccctttgcac
    64561 aacagcctga acatcgctgt cgaattcttc caaaaagagg tgacaatgtg tgtcaatcaa
    64621 ttccatagtt cttttttcaa aaataagaat tttatatcaa tggcaggggt ggtaaaaaaa
    64681 cagcctgact tgtaaatagt caggctgcat attttattgt tgcgaaacat catttttacg
    64741 ttctgttttt tttacctcag gtttctcagg ctttggaaga agaactttgc gggacaacct
    64801 aagcttgtta gtctttttgt caatctcgat aagtttaact tcaatctcgt caccttcttt
    64861 caaaacatca gcaacgttct caatacgttt ccagtctatt tcagaaacat gaagaagtcc
    64921 atctttaccg ggaagtatct caacaaaagc gccaaaagca gtcaaagtct taactttacc
    64981 tttgtatatt tctccttctt cagggacagc aacaatcccc ttaatccacc tgatagcttt
    65041 atcaatagat tccttattat cggcaaatac gtcaattaca cctgagttgt ttacttcttc
    65101 aatagtaata gtagcgcctg tttgacgttg tatctcctga attactttac caccaggccc
    65161 gataacagcg ccaatatatt cgcgggctat tgtcatttgc tcgatgcgtg gagcatgtgg
    65221 tttcaagtcg gcatttggtt gggcaatagt atcagttatt ttaccaagga tatgcaaacg
    65281 gccttcttta gcttgttgca atgcctttgc cattacttcg tatggaagcc cgtcaacctt
    65341 gatgtccatc tgggttgctg taataccttt atcagtaccg gcaactttaa aatccatgtc
    65401 acctaagtgg tcttcatcac ctaaaatatc agaaaggatt gcatactttc ctttgcctga
    65461 gccattttcg gtaataaggc ccatggcaat accagaaacg ggacgtttaa ttttgatacc
    65521 agcatccata agcgccaatg taccggcaca aacagttgcc atagaagacg aaccgtttga
    65581 ttcaagaata tccgaaacaa cacgtatagt ataaggattt tcagctcctg taggtataac
    65641 atttttcaat gcgcgatgag caaggttacc atgccctacc tccctgcggc ttaacccacg
    65701 tgaagctttg gcttcacctg ttgcaaaggg tggaaaattg taatgcaaca aaaacttgtc
    65761 ggttccttgt atcatcacat cgtcaataat tttctcatcc agcttagtac cgagagtaac
    65821 agtggtaagc gactgggttt cgccacgagt aaaaatagaa gaaccatgag cagcaggcaa
    65881 acaatcaact tcgcaccaaa taggcctgat ttcgtttgtt ttacgcccat caagacgaat
    65941 cccttcgttg agaatcatgt tgcgcatagc ttctttttct acctcatggt aatagcgctc
    66001 tacaagcatt ttcttttctt cttgttcttc ttctgaaagt gaaagcaaga aatcttcttt
    66061 aattttctca aaagcttcgc tgcgctcgtg ctttggacgt ccagattttg caacctgata
    66121 aactttgtcg taaagctcac tgtgaatgcg tttacgcaaa tcgtcatcgt ttacttcatg
    66181 acaatattca cgtttcttag ttttaccaac aagttcggta agctctaact gtaccttaca
    66241 atgttttttt atttcttcgt gggcaaattt aatagcctcg agcatttctt cttcactaac
    66301 ttcgagcatt tcgccttcta ccatcaggat gttttctgca gtagcgccaa ccataatatc
    66361 aatatcggct tctttgagct gcgagtaggt tggattgata ataagctggc catttatcct
    66421 ggctacccta acttcagaaa taggcccatt gaaaggaata tcagatacgg ccaatgcagc
    66481 agaagcagct aacccagcca atgcatcagg catagtatct ttatctgcag aaattaagtt
    66541 aaccgtaaca aaggtttctg catgaaaatc atccgggaat agaggcctta aagccctgtc
    66601 gatgagacga gaaactaata tttcataatc agaggctttt gcttcgcgtt ttaagaaccc
    66661 tccgggaaaa cgtcctacgg cagcatattt ttctttatat tctaccgaaa gaggcataaa
    66721 atctgtatct tctttggctt cttttgctgc aacaacagta gcaagcaaca tggtgttgcc
    66781 cattttaacc actactgaac catcagcttg tttggcaagt ttaccggttt caatggtgat
    66841 tacccgtcca tcagttaata caatatcttt agaaataaga ttgtacatgt ttgaatgttt
    66901 taataaatat taaatacaaa aaaaggcaat tgatattgcc tttattttct tatattaagc
    66961 gctttaagga tacttctgta ccgctcaata tctttttctt taagataatc aagctggcga
    67021 cgccttgcgc caaccagttt aagaagagca cgttgtgtac tgtaatcttt atggtttgct
    67081 ttcaaatgtt cagtcaaatg attgatacga gttgtaaaca aagcaatctg actctctgca
    67141 ccaccagtat tagtagctgc accaccaaat tcagcaaaga cttctttttt cttttcgctt
    67201 gttaaataca tattaaaacg agtttttatt cttatttttt agaagcgcaa agttataaat
    67261 attttttaat agaaatgatt atttttcaaa cgattatttt tttgacaaga taaaaaattg
    67321 tttgtttgtg cttattttta tcaaaatatc cataaatttc cttatgcgta aagtatttct
    67381 ttttcttctc ttcattagcc ttataaaaca aggcttttca accgaaaaga tcttgttttc
    67441 aaaattggat atcaatgccg ggttgtcgca aaatcacata aattgcattt ttaaagactc
    67501 aaaagggttt gtttggatag gtaccatgtc cgggctcaac aggtatgatg gttctttgtt
    67561 taaaattttt cgtaatgacg caaatgattc ggcttcaatt attgacaact ttattattag
    67621 tataactgaa gataattttg gctgtttatg gattcttacc cgcttaggtt ataacattta
    67681 caacccggtt gatgaatctt tttctcgtca agcccctgaa tgcttttcag caataggttc
    67741 tgattttacg caaatcaatg aaatgactaa agacatagac ggagggctgt ggtttgccca
    67801 atctaacctt ggggttattt actacaatcc acttgaaaac aaatgtgaac tactgaaagt
    67861 aaaccccaaa aatacagaat gtttgtcttc aaaggctgtc cgtgcaatta caaatgatac
    67921 agaaggcaac ctgctgacca taaacgacaa cgcagtaatc gaaagaattg accacaaaac
    67981 aaaaaaagta acccaacgca ttaccagcat acgcaatatc tgtggcgacc ttgcttttta
    68041 taaaataatg tgcgacaaag aaaaccttat ttgggtttac agcaccaatg tagcttctgg
    68101 aatttatcag ataaacccca ggacattgaa aacccgcatt tttaacacta aatcattgcc
    68161 tgcattaaat aataatatca tcagggctgt tgaagacgat gggcttggac gtatttggat
    68221 tggcacagac catggaggta ttaatattat tgataaaaaa gctgataaaa taactgttct
    68281 cacatcagac aataacaacg aatacagcct ttcgcaaaat gcaattacct gcatttataa
    68341 agacaataca aatattgttt gggtaggaac atacaaacgg ggggtaaatt tttaccaccc
    68401 taaccttgtg aaattcgact tgtatagaca tgaaagctca aaggcaaaca gtattggctt
    68461 tgatgacata aactgttttg ccgaagataa aaaagagaat ttgtggatag ggacaaacaa
    68521 tggaggtatt ttttatttcg acaggaaaac caatactttt aaaaactaca aacataaccc
    68581 atctgatcct gcaagcctcc ccaataacgt gatccttaaa ctatatattg acaaagatga
    68641 taaactttgg gtagcttcat attacggagg attaagttat tttgacggac aaaagtttgt
    68701 acgctttaaa catgacgaca aaaaccccca aagcttatca gacgataaag tttgggacat
    68761 ttttgaagat tctcaaggga atttttggat tggtacccta ggtagcggat tagatttatt
    68821 tgatcgcaaa aaaaacattt tctaccatta taagtatggc gaggttaact ctgtgagttc
    68881 taattatata ttgcatattg ccgaagataa aaacaggaac ctttggttag caacagcttt
    68941 gggagttaat atgttagaac acaaaacagg gcggtttgtt tactttaaca atatcccctc
    69001 agacaaaaac agcctttgta ataataatgt aacctttgtt ttccctgaca gcagagggct
    69061 tatatggatt gggacccgcg aagggcttag tatttataat ccttttacac gtaagataaa
    69121 cagttatttt atgaatgatg ggttgccaag caataccatt caaagcatta cagaagacaa
    69181 cctgcaccat atatggatta gcacaaccaa tgggttgaca aggtttttta taaaatcatt
    69241 taccaacaat aagctggaat ttgatatttt caactatgac aaatctgacg gtttgcaggg
    69301 aagcgagttt aacgaaaaag catgtcttaa aacatcgaaa ggagagcttg tgtttggcgg
    69361 ggctaatggg tttaacattt tcaaacctca acaaattaaa agcaacacca ctccccctcc
    69421 ggtatatatc actgatttat acctatttaa cgagaaggta aaagtaaata aacctgtttt
    69481 taaaagaatt gtactaacca aatctttgat gaacaacaaa aaattgaagt tacgctatag
    69541 cgaaaatatc ttttctatcg aatttgccgc acttaattat cttcatcccg aaaaaaaccg
    69601 ttttaaatat aagcttgaag gatttaatga aacctggatt gaaacaaata gcctgcatcc
    69661 aaaggctacc tataccaacc ttgaccctgg taggtatatt ttcagggtta tagcatcaaa
    69721 tgacgatggc gtatggaata aagaaggagc ttcgctcgag atagttgttt ctcctccatt
    69781 ttggcgctca gtcattgcat ttgtacttta tttcctgata atttttggga tactgatata
    69841 cgcaaggcac caagttgtaa aaaaagcccg gataaaattt agcctggaaa atgaacgcaa
    69901 agaagccaaa cgtttgcacg aactggacat gctaaaaatt cgttttttta ccaatatcag
    69961 ccatgagttt cgaactccgc tcacactgat tatcacccca ttggaaaaat tactaaaaac
    70021 aacacaagac acaatactgt taggacagtt aaacctgata caccgaaacg caaaacgcct
    70081 gctaaatctt gtcaatcaat tacttgacat tagaaggctt gaagttgaac cgtttaaact
    70141 gaacctgcaa ttaggcgacc tgatatattt tgtccgggac atcaccacct ctttttcaga
    70201 ctttggcgaa aagaagaaca tccagcttat tttcaaatct aatgttgaat atatcaatat
    70261 agcttttgat gccgacaaaa tggaaaaaat tctttttaac ctcttatcca atgcctttaa
    70321 atttacttat gaaaatggaa aaattgaggt taacatagaa tacaacaaat taggagacaa
    70381 tagcaaggat gctgttacca taaaagttaa agatacaggt attggtatcc ctcacgaact
    70441 tcaggaaagc atttttgaac catttattca aaatactgaa atgccggcca acattattaa
    70501 ccagggaagc ggaattggac tggccctgac caaagaattt gttcgtttgc acaatggccg
    70561 catttgggta gaaagtgagc ccggcaaagg gagctgcttc tatgtagaac tgccggtcaa
    70621 tgtatctgca gaggctgttg gtggcaacaa tattgagaca gtgttaatca atgctgaaaa
    70681 agaagccctc cccgaaaatg agacggacga accttcaaat aaaacaaaat cttcgttgat
    70741 gttggtagaa gataacgaag atttaagatt ttacctgaaa gacaacctgc ggattcatta
    70801 ccagattttt gagtcaggca atggaactga tgcattaaag caaattatgg aagtagtacc
    70861 cgatttggta gttagcgata ttgccatccc cggcattgat ggcattgagc tttgtaaaag
    70921 gctcaaaaat gacaaacgca cctcacatat cccggtaata ctactcacag cccatcttga
    70981 caacactcaa aaagtaaatg gatttgaagc tggtgcagac gattacctga caaagccatt
    71041 tagctttgaa attcttgaat caaggataaa aaaccttatt tcactaagag attctttacg
    71101 aaaaacattt aaaacgaatt tcgatcttaa cccctccgaa gttgaaatag tttctttgga
    71161 tgaaaaattt ttaaagaaaa cccttgaagt ggtagaaaaa caaatcggca atcctgattt
    71221 ttcagttgaa gacctgagca aagaaatggg catgagccgc gttcatttgt acaaaaaatt
    71281 gcttttcctt acgggaaaga caccaagtga atttataagg cttatccggc ttaaacgggc
    71341 tgcccagctg ctccaaaaaa gccaacttac agtagctgag atttcatatc aaacaggttt
    71401 taacaatcca aaatattttg cccggtattt tagagacgaa tttggtattt taccatcagc
    71461 ttatgcccac gaatataaaa ataaacaaga ataaaactat ttgtcaaata tgccttcctt
    71521 taaaaataat aacaatagta taataaataa ccagaaaatc tgtacgtaac gacatatttt
    71581 cgagatatat aagatcgtaa cgcaaacgtt ttaccatttg ctcaatattg cttgcatatc
    71641 cgtacctaac ttgccccaat gaggtaatac caggttttat cttttgcaac tgtttgtagt
    71701 tgggagcaat cttcactatc tgttctatat agtacttgcg ttcaggacga ggtccaacca
    71761 aagacatatc tcctttcaaa acattataaa aattaggtat ttcgtcaagc ttggtttgtc
    71821 ttagcaaacg cccaaaaggc gtacagcgtc tgtcgttttg agtagaaagg ttaggtccat
    71881 ttttttcggc atcgacatac atactcctga acttgtaaag aacaaaaggc ttgccatttt
    71941 taccaatcct ttcttgcctg aaaaatatag gccctttaga agttaccttc acgccaatag
    72001 caataatgat aattaaaggc agaaaaagta aaatagccaa aactgccccg gtaaaatcaa
    72061 tacatgcttt taaagcctgc tgccacaaag gcatcaactg atgatcaatt tcaaataaag
    72121 ctgtacccag gatattacta attttcagct gtcccttcaa atgactgcta atctcagcca
    72181 caatttttac agttatttca gaataaccca accagttaag gatttctttg gtcttttcac
    72241 tttctgatga ttctgtagca atgataattt ctgacgggct atattctttt ataatgtccg
    72301 ggaaatcctg aatagtaccc aaacatggca gttcattaaa ctttgaatta ttgtcactgg
    72361 tttggatata cccgataaac cgattgccat aaatagcctt tttgagcgtc aactcgtcaa
    72421 aaatattttt tgcctgttgc tgacaaccta ctatgatggt atcaaatgaa agctgaccat
    72481 ttcgtacttt tctttgcttt gaagcggtaa ttatccaacg tccggcaaga gttagcaagt
    72541 attgaattgc aaaaagcgta ataaaagtga tgtaataatt tttgtaattc cgaacctggt
    72601 catcgagcaa gaaaaaaaag aaaataacaa cagtgccaat aaaaacagcc tggaaagaag
    72661 aaacggcatc cgtactttta tttttattat aaataaagtt ataataacca gacaaatagt
    72721 acaaaaaaac ccaacatagc ggtataaggg taattcccag ataaaactta ctgtcatgca
    72781 acatttgcga cacaacctgt gaaaaatcaa actgctcaat aacatatttt cgaaaaacga
    72841 aaaaacatgt ccaggagatg acagccataa aaaagtctgt tgcgatatat aaagcttttg
    72901 cctttcttgg gtttgtcatt gctcaaaagt ttattcaaaa atagtttttt ttcaataatt
    72961 aattagcatt ttcaggtatt tgcttctttc ttattacgaa aagccccata aaagtcaaca
    73021 ttccattaat aataagaatc tcaaagctaa agaaatcggg gaacaaaaag tttaaaaata
    73081 aacaaacaac cggagaagca atagttacat aagggactaa tttttctttt aatttatatt
    73141 ttgtaaacaa cccaaaagaa aacaggccaa gcagcgggcc atacgtataa ccggcggcgg
    73201 taaacacagc tgatataaca ttttggttat ttacaacatg aaaaagcatt attagcccga
    73261 taattaaaag agaaatcgcc agatgtactc tttttcttgt ctttgcaaga tattcatcac
    73321 tcttttggtt tgctttcagg atgtctaatg taaatgaagt tgtcaaagct gtaatagcag
    73381 agtctgaact ggcaaatgta gcagatataa ggcctattgt aaagattatt acaaaaaaca
    73441 caggcataac atctaatgta gcaacggcag ggaataacat gtccgaattg gcaggaatga
    73501 caaaacctgt ttgggctgca aatatataaa gcatagcccc tacaacaaga aacaaaatat
    73561 ttacaggaat taggctaaga ctaaaccaaa acatgttttt ttgagcctca cggatattct
    73621 tgcaactaag gtttttttgc atcaaatcct ggtcaaggcc ggtcatagtg atagcaataa
    73681 aagccccgct cagaaattga cgaaaaaaaa agcgtttatc gctatactcg gtccaaaaga
    73741 aaactttgga ataattacta tctctgatgg cattgaaaac ccctgcaaaa tcgagatgaa
    73801 ggaacctggc cagaataaca accaccccta taagtgagaa taacatgaaa gccgtttgta
    73861 tggtgtcagt gtaaataata gtcctaactc cgcctattct ggtataataa taaacaaaaa
    73921 gcaaggcaca acttgctgtt agccaaaaag gaacacccat gtggtcaaaa acaacatact
    73981 gaagcacact gaccgaaaga aacagcctaa aagcagaccc tatagttctg gaaataagaa
    74041 aaaacatagc ccctgagatg tatgaagttt ctccaaaccg tttttgcagg tatgtgtata
    74101 tagatgtaag ttcaagcttg tagtataaag gcattaacac caaagctata aaagcatacc
    74161 cggccagata gcccaataca atttgcatat acgaaaactg gctggttcca acccatcccg
    74221 gaaccgaaat aaatgaaacc ccagaaatag aagccccaat cattccaaaa gcaacaataa
    74281 accagggcga tttacggttc ccgataaaaa atgttgaatt atcagctttt cgggcagtaa
    74341 catgggatac aatgtaaatt ccaacaaaat aaactaatat gatacaaagg ataaaatatg
    74401 gagacatcat tggtaaagct ttacggcaaa tgtagaaaat cttttaaatt tacaattatt
    74461 tataattttg cgataaacag attttatatg aacagcccca ctttttcgtc tcaattgttg
    74521 gaggaagctg tagagcaatt tgccaaacta cccggaatag gccgaaaaac agcgttaaga
    74581 atggcgctat ttcttttaaa acaacccatt gaagatgtca acctgttttc ggcttcgatt
    74641 tccaggctcc gaaacgagat taagttttgc atgatatgca gtaacctctc tgatgctgac
    74701 gtatgtccta tttgcaacga caagcaacga gacaattcgc tggtttgcgt tgtcgaaaat
    74761 gctaaagatg ttctttctat tgaaaataca ggacaatatc atggggttta tcatgtactt
    74821 ggcggtatta tttctcctat ggaaggcata ggacctgccg atttgcgtat tgaacagctc
    74881 gaacaacggg tggcagaagg gactataaaa gaaataatat ttgcttttag tgctacagca
    74941 gaaggagaca ctacctcgtt ctatttatac cgtcgtttaa gccgcttcag tgtacaaata
    75001 tcatctatcg cccgaggagt tgctatcggg gacgaattgg attatacaga caccgtaact
    75061 cttggccgat ctatacaaaa cagattaccc ttcgaggtaa aacaaaacaa atagaacctt
    75121 atgtataaat taaaatttat tgtcgcatta gcagagctgt ttgtattggc ttttatagca
    75181 aaaagccaaa caaaccctga acttgccaat aagataaggg ctaacgtggt tattggcatt
    75241 ggcaatgaag ctttgccttc atttttcgac cctatccctt cgggtgatga aaatataatt
    75301 tatactgtgg gtgtgtctga cccatacgcc aatgattcgg tagctttgat gcaggcaaaa
    75361 gcccgtgcag tactgcttgc ctcagtactc aatggctgca ggttctcggg agtttttgac
    75421 aactataaca acagcggcaa tacaagcaaa ttgcaggaat tgtacaggat aaaatcagca
    75481 gttccgctga ttggttcatt aacaatagtg gacagcctgc aaacccgctt taatgaaaaa
    75541 atattactga taaaatttga caaaacaggc aaagacacct tgaccaaaga agtattgata
    75601 gagcttttca agactcaacg aaaattagac tatggcacac agatagatta caacatcaag
    75661 gtagaggaac ggtacaacaa tgttgacttt ccttacattt ataccaatgt agatggctca
    75721 tttgagatac tttcaaagtt ggacaataac aaatcaaagg tacccccagg gatttatcaa
    75781 tatgaacaaa attcttcgga ttcaacaaaa aatgttgtat atttacaaca caaagggctt
    75841 tggtgtggaa tattgcaagc ttttacaaat gaactggccc ttcaggcatc cacatcaaaa
    75901 tccgtaaaca ggcaagtagg tcaaaattcg ggggacagct acatgggata tctttcgcgg
    75961 gaaattttga ataatgcctt tacaagcaga ataataaaaa tatttatcaa cttagaccaa
    76021 ttacaaattt cattgacaaa aattcaaaaa ttatagttat cattgtattt taaatcctta
    76081 aatctaaatt tatgaaaaag attttatacc taacattagt ttcagcagca ttagtattgt
    76141 tgaatacaag ttgtggcggc cccaaaaaaa tgtccgcaac tgaagattct caagaagtaa
    76201 ttttaccttt atcaggaaaa gaattccaat ctgacaaaga gttttacaga gcaaaggctt
    76261 ctggaaagag cccagatatt gcaacagcaa aaaaaatagc tttaaacaat gctaaagctg
    76321 agatagccgg gctaattagc agtaaaatca aaactgttac agacaactac accaaccaac
    76381 gttctgtagc tgatgcccag gatttcgaaa gcaaatttga aacaatgacc cgtgaagtag
    76441 ttaatcaaca attggttgac gttgctatta tcggtgaaaa attgatcaaa aaaggttcaa
    76501 cctacgacta ctggattggt attgaggttt caaagaaagc aatcttagaa ggtattaata
    76561 acagcatttc taagaaccaa aaacttgcta tcgattatga taaaaagaaa tttgaagaaa
    76621 tctacaatgc cgaaatggaa aaaatggaag ctcagcaata atctgagagc ctaaaaatat
    76681 gagaggctgt ccattggggc agcctttttt tgtactaatg cttaaaattc agacacatcc
    76741 tcatttttag ccattattat tcacgaggca atgttgttta gttaagtatt aaaaaagacg
    76801 ccgacaagcc agaaagacgc tgtcggggta gtctcacatc agacctgaca gctttcgcag
    76861 aacctgccaa agtcttgact aaacaacatt gattcacgag gcttcaatat aacctgacat
    76921 tgaatgatac gtaataattt tttcttttac cccaaaaaaa gaaaacctca taaagcaaat
    76981 gcttgtagcg tgcatttttt atcaacaatt atcccaatac accttatgcc ggtttttaaa
    77041 tttatgcatt taacccaaat tataaggagg ctttttaaag ccaagttata tgatttagcc
    77101 ggggtaaccc tcccgttaaa aacgggacaa gctgatttta gaaactgttt gatattttgt
    77161 taaactattg aactgcaatc acttaatcaa ccaagtatta ttaatgctta atttttggat
    77221 taagttgtta aacgatacac ctgcaaaagt tatcaaaagg ttttacaata tgacagcctt
    77281 gcctaaaatc ctgttttatc taatattaac ccggtagctc cacggccatc ggcaacataa
    77341 tcgcggctta ttgtacctgc tttttctaaa aagactttat cagtatgtat tttgtcaaga
    77401 atttttgtta attcgttaat gtttgcaata ggaaaagctc cgcctttttc ggcaagttct
    77461 acagcctcgc gaaacttttt ataattgggg ccaaaaacaa caggaatacc aaatgtagca
    77521 gcttcaagta tattgtggat acctttgcca aagccccctc ctatataagc agcatagcca
    77581 taccggtaaa cagacgacag cataccaacg ttgtcaatga tgagtacatc gtattcagaa
    77641 aggttaactt tttctgcctg cgaataccga ataacttttt tattgatgct gctgacaatg
    77701 ctgtttatcc cgtcttcttt gatttcgtga ggggcaaaaa taagcttgac cgggtttggg
    77761 tatgcgttaa aatactgaat aatcagtttc tcgtcagcag gccagctgct tccagcaata
    77821 aacgccttat gtccgttgat aaatttttca atcacaggca gttgctttgc ttgcgaagca
    77881 agttcataaa ccctgtcaaa tctggtatct gaacaaattg aggtatttgt aatacctata
    77941 ctggccaaaa gctctttaga ttgttgattt tgaacaaaaa tatggttaaa atactttagc
    78001 atactgcggt accaaccacc ataccatttg aaaaacactt gctgaggcct gaatatggat
    78061 gaaaaacaat aagttggtat gtttttttgg tgcagttgat taagatagtt gtgccaaaac
    78121 tcatatttga taaaaaatac tgctttcgga ttagccaatc gaacaaattt acgagccatc
    78181 caggtaaaat cgaacggcaa ataaacaact acatctgctc ctgcataatt ttttctaatc
    78241 tcgtatccag aaggcgaaaa aaatgtcaga agaatagtta catttttatc tcttcgtttt
    78301 atttcttcta taactgggcg accttgctca aattcgccca aagaagccgc atgaaaccaa
    78361 ataacatttt tgtttttcag atcagctttt tttaaagtgc caaaaatacc tttgcgtcca
    78421 ataagaaatt ttctgatttt ttcatcaaaa agcgatgcaa cacaggtgcc aaaatagaat
    78481 aaggccaaaa agatatgata aaaaaaagtc atactttgtg tatagtcagt taatttgagt
    78541 gagcaaagaa acaaatattt tatgatatgc cctttgtttt ctttaaaaaa tgatttttaa
    78601 agttgaattg aattattgaa ggatagtttt tttagctaaa ataattcatt aactttaaaa
    78661 gtatgaagaa tagaaatatt ctgccatttt tttaaaactc atgtaccaca cgcctgaagg
    78721 cggtgggttg ttctaacgga ctgtaag
//
LOCUS       DAOSPF010000006        78161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_328_length_78161_cov_6.740929, whole genome shotgun sequence.
ACCESSION   DAOSPF010000006 DAOSPF010000000
VERSION     DAOSPF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 78161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_78161_cov_6.740929"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK252_02060"
     CDS             complement(<1..370)
                     /locus_tag="PK252_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HOT13520.1"
                     /translation="MKVKLKILDRYLTLWIFLAMFAGVLMGWLNPDIAQFWNHFSSGT
                     TNIPIAIGLILMMYPPLAKVKYEELGDVFKNTKILGLSLVQNWVIGPILMFLLAIVFL
                     QFNIDYMYGVILIGLARCIAM"
     gene            complement(419..766)
                     /locus_tag="PK252_02065"
     CDS             complement(419..766)
                     /locus_tag="PK252_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT13521.1"
                     /translation="MRNDKENLIVSLTLEFALEIIEYAEILEDHKKYIISKQLLKSGT
                     SIGANVREAQNAESKADFIHKMKIAAKEADETEYWLTLCELSKNYPSPNGLKESLQSI
                     INVVSKIISSSKS"
     gene            complement(849..1274)
                     /locus_tag="PK252_02070"
     CDS             complement(849..1274)
                     /locus_tag="PK252_02070"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT13522.1"
                     /translation="MKILILCTGNSCRSQMAHGFLQSFDKSLTVCSAGTQASGKLNEK
                     AVAVMKEVGVDISHHTSDSVDKYLNEEWDYVITVCGGANEACPAFLGKVKHRLHIGFD
                     DPSHATGTEEFIWSEFRRVRDEIKEGFYKFYQENLKIWK"
     gene            complement(1334..1669)
                     /locus_tag="PK252_02075"
     CDS             complement(1334..1669)
                     /locus_tag="PK252_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT13523.1"
                     /translation="MNKAEIFDNDFKELARFAKAISHPARLAILKYLAETKTCISGDI
                     SECIPLSRSTVSQHLKELRDLGLIHGEIDGLKVNYCLCSSNIEKYLKLFDNFFGPISK
                     QNFECIKTK"
     gene            complement(1752..3392)
                     /locus_tag="PK252_02080"
     CDS             complement(1752..3392)
                     /locus_tag="PK252_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOT13524.1"
                     /translation="MDNYKNIIREIAQINHSTGAPELTKPVESLLAENKKAIHIAVLG
                     QFKSGKSSLVNNIIGENILPVGVVPVTAIVTRLQYGPEPKLTIRFTDKEDLTTTLNEL
                     PLYVTEKHNPENNRNVSLAIVEHPILEPFKNISIVDTPGLSSFYRHNSEATLQWLPFT
                     GVAIISVSAERPLAEEDLNLIKGVMLHCPNIALVITKTDLFKTNEIEEIKAHIRFSLQ
                     KAISRDIPMFEYSVFSQLTIFRDTLINNFINPFNKDYEKEYDEIIRYKIQNAISQSIR
                     YAELALQTALKRENEKEAINKVLKEIKANRHHHEREMLLSGSSFKGETRDKLEKIVLP
                     NQAKVAQQLEEQFYAAYQTWNGSLFNVSRSFEQWLKANLGHQITTIDNACFEQINQIV
                     KEIASYYEYSALQYRQRLNDRINQMFGVNLPEVSWQIEFSGIDKPDVSVYRAFDSHLD
                     TLLFFLPMRWFKRLFYHHFEKQIPNETDKNLHRYISGLTEKIIKSIDAIHKQALMFII
                     NENKDVEHILLNDKNNHIELQRFIEQLKEIKSLTIKTN"
     gene            complement(3405..3818)
                     /locus_tag="PK252_02085"
     CDS             complement(3405..3818)
                     /locus_tag="PK252_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT13525.1"
                     /translation="MWWVYALLSAFFAAFTAILAKVGIKGINSDLATAIRTVFILFIA
                     WGIVLARKEIYGINALSKSNWLFLGLSGIATGLSWIFYFKALQLGKVSQVAPVDKLSV
                     AIALILSSLLLHEVITWKILLGTILIIAGSIVIIL"
     gene            complement(3828..4544)
                     /locus_tag="PK252_02090"
     CDS             complement(3828..4544)
                     /locus_tag="PK252_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT13526.1"
                     /translation="MLFILFILFIGSWLAASISGIAGFGGALLLLPVLTHFIGIKAAI
                     PVLTIAQLFGNASRVWFGRKELQWQPIAYFLAGALPFSIIGGRILVVSSTVYLIKGIG
                     FLLLMMVIYRRLGIKFFTAKNRWLFVGGIATGFLSGIAGSAGPLGAACFVSLNLTPIA
                     YISSEAFTALLMHISKTVVYQKYSLIGFNELGYGLVCGTAMILGSYTGKKMIAFLSRE
                     KFLLLVDSLLVLSGLQMIFF"
     gene            complement(4544..5122)
                     /locus_tag="PK252_02095"
     CDS             complement(4544..5122)
                     /locus_tag="PK252_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT13527.1"
                     /translation="MIQSLNSLDTQLFLFLNGKHNAFFDPIMYWFSDKLIWIPMYLLI
                     AFFIVKHYKMKGVVMLLLVGVVIALCDQTASHLIKNAVQRLRPSHEPSLAGLVHLSKA
                     GAGGMYGFVSSHAANAFGLATFLFFALDNTFKYLKYWLFAWAVLVSYSRIYNGVHYPG
                     DVIVAAIIGACYGFIFAFIYLQFDKKMGKQSN"
     gene            complement(5122..5709)
                     /locus_tag="PK252_02100"
     CDS             complement(5122..5709)
                     /locus_tag="PK252_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT13528.1"
                     /translation="MKKYLIFLLLLSPIVLYSQNIDIDILKDINLNRNKNLDGVFRVI
                     TDSASTVAYGLAVLLLVIAFRRKNIDARQRSVYVAVSVTTTAIIATILKYSIHRPRPF
                     ITYPFLEKVTSGGSPSFPSGHTTDAFAIATAVSLAYPKWYIIIPSYIWAIAVAYSRMC
                     LGVHYPSDVLVGAFIGIITATIWYKEKHLLYKKIK"
     gene            complement(5858..7021)
                     /locus_tag="PK252_02105"
     CDS             complement(5858..7021)
                     /locus_tag="PK252_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT13529.1"
                     /translation="MKFIKKTPWAFVLLIGIVSLFSDMTHEGARAITGQFLGILGASA
                     TVVGFIAGFGELMGYTIRLASGIIADKTKQYWTITFVGYAINLLAVPALALAGNWQIA
                     AVLMILERTGRAIRNPARDVMLSHASSSIGHGKAFGVHEALDQIGAVTGPIIVGAVFF
                     FSGNYAKSFGILLIPALIALTILVVARLNYPRTEHLEDKNNMPKLVFPKKFYWIYLAA
                     VSLIAAGFADYPLIAFHFNKTGQVTTQWIPVMYAIAMGVDALAALLLGRLFDKHGIKV
                     LIGSTLLSLLFAPLVFMGNFYTAIAGMVCWGVGMAAQESVVRAVLSSILPADKKATGF
                     GVFNAVFGLFWFLGSFLMGWLYDMSIIWLIAFSVVMQLASVPLFVKLSSKNLN"
     gene            complement(7040..7261)
                     /locus_tag="PK252_02110"
     CDS             complement(7040..7261)
                     /locus_tag="PK252_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13530.1"
                     /translation="MISIVDNFVILFSFTYKAKSLHRVIKAPAVIVLHTCQLDGFMFF
                     FIMLHEHLRSTIILGQNTLLPIKPAFFIV"
     regulatory      complement(7285..7347)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7444..8724)
                     /gene="eno"
                     /locus_tag="PK252_02115"
     CDS             complement(7444..8724)
                     /gene="eno"
                     /locus_tag="PK252_02115"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT13531.1"
                     /translation="MKIIKVEAIEILDSRGNPTIEVNLELEDGTKARAMVPSGASTGE
                     REAYELRDGDKKRYGGKGVLKAVENVNKVIAKEIEGKCFVSQRELDYFMINLDGSQNK
                     SKLGANAILGVSMAYARAKAMSAGMPLYQYLGGSNAYLLPVPCMNIINGGKHADNNVD
                     FQEFMIAPHGAPSFTEAIRMGVEVFHTLKGVLKAKGYSTGVGDEGGFAPDLKSNEEAV
                     EVILEAINKAGYKPGNEVSICIDPAASELWEDGKYKMFKSTGKLITGDELIKLWESWT
                     NQYPIVLLEDGLAENDWDGWKNLTKALGNKIEIVGDDLFCTNKAILAEGIEKGVANSI
                     LIKLNQIGTVTETLETIELAYKNSYNCFVSHRSGETPDTFIADLTVAVNAGHLKTGSG
                     CRGERIEKFNQLMRIEHELGKNSKFAGIAAFKNR"
     gene            complement(9052..10560)
                     /locus_tag="PK252_02120"
     CDS             complement(9052..10560)
                     /locus_tag="PK252_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13532.1"
                     /translation="MKVFLMFEKDNFETRKKLLPNSAALVQDLELGTLFATMASGDEF
                     IYKIAKEALLSGTFNDIETILYRQDILKDSIENQLVVREIYRLSTEALLSRNEHWWFL
                     SSSPSSVLYSSIASLKDLLGYLKQLKALADEHASHFQSKGFSRFFAMLQKELDEDFMA
                     EAWKHIKELEFHDGALFNTQLGKGNKGYPYQLCKLPQQKQGWVQMLFPRKKPGYTFNV
                     HPRDESGARALREIKDEGVNVVANALAQSMEHIFSFFSMLKTEIAFYTGCLNLHGQLA
                     SLGADCSFPQPLPGHLRTHVFRGLYDVSLALAMKQKVVANDAIAENKNLVIITGANQG
                     GKSTFLRSIGLAQLMMQCGMFVPAASFSANTCSQLFSHFKREEDSAMNSGKLDEELSR
                     MSSIADNLSSHSMILFNESFAATNEREGSEIGRQIVNALIEKGVKVFFVTHLYDFAQS
                     FYVRKLENALFLRAERQADGARTFKLVEGEPLQTSFGKDLYNKIFDNPDAGI"
     gene            complement(10565..12097)
                     /locus_tag="PK252_02125"
     CDS             complement(10565..12097)
                     /locus_tag="PK252_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13533.1"
                     /translation="MVFESILFASTVPDDSLHLEEPAFMVDLNINQVVDTALKGKEEY
                     NLKPYFYTLLANTEDIKYRQEVMQDIERQDLYRYLKAFEGDMRNIRKQLAETEKLYYQ
                     YHKERVFLDIVIFYGQAMESLAKNLPHLPIKSRGFITFCNYLTSYTESLQFKNLLAEA
                     RAIIHELSSIKYSVHIKELRVRVLDFQPGDDYTEEVQATFAKFKQGDVKDYRSGLSYP
                     LGMNHVEAKILDGVAYLNPDAFARLDAFYKGNTRFQDETIVRFDREVQFYLAYFEYIE
                     NIKKAGLEFCYPQVSAVDKEIDVTACFDLPLAHKLVSEKKAVVANDFYLKGHERILVV
                     TGPNQGGKTTFARTFGQLHYLAGLGLPVPGSRAKLFLCNNIFTHFEKEENVLNLRSKL
                     EDDLARLHWILGHCTPDTIVIMNEILTSTTLQDGIFLSKKIMEDLTRLDVLCVWVTFI
                     DELASFNEKTVSMVGAVVSGDPSQRTFKFSRMPANGLAYAISVAEKYQLTYSELKKRI
                     IP"
     gene            complement(12157..12627)
                     /locus_tag="PK252_02130"
     CDS             complement(12157..12627)
                     /locus_tag="PK252_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOT13534.1"
                     /translation="MIAIDIPGYKKIELAHLVLDYNGTLAIDGKLIDGVKQLIGQIGS
                     KLNIYVLTADTFGSAKGQLSGLNCSIEILGLSMQDIQKEKYTISLGAENVVAIGNGAN
                     DALMLKSSALSIAVIQKEGASAKSIMAADIVCFSIVDALELLLNPKRIIATLRR"
     gene            complement(12651..13892)
                     /locus_tag="PK252_02135"
     CDS             complement(12651..13892)
                     /locus_tag="PK252_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOT13535.1"
                     /translation="MILNKYQCGLDFGTSNTAIAITDIHTRKSIFIENDSSVLHFKNT
                     NDYVYSVGKAAINEYVSSDMSGRLLKSIKTLLKQKNFIHTFIFGKRFTPDELAALIIK
                     HFKQQAEKAIGHEIEQVVLGRPVVFSEDPEKEEIAVKRLLKAASNAGFKDVKLLYEPI
                     AAAFSYESSLTQHERVLVADFGGGTSDFTIMELGPDRINAEDRKGDIIANGGLYIGGD
                     MFDSQTMWHKLTPLLGRGVKYASYNKELDVPTTIHHELKKWERSFLLKETKLRRDLNN
                     YYIFSGRDKRLEDLMALIDNNLVYALFQQIEKAKIELSSKTETMVEFARNIINIRELM
                     TLEEFRNFINNETNKIEHCVLSLMENANVKAADIDTVFLTGGSASVVPIRDIFEKLFG
                     PHKIRMGDNFNSIAYGLSLSK"
     gene            complement(13914..14381)
                     /locus_tag="PK252_02140"
     CDS             complement(13914..14381)
                     /locus_tag="PK252_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOT13536.1"
                     /translation="MEIERKFIVDIENWQNIAKGKGERIIQGYLYSSIDKTIRVRVMG
                     DNGFITIKGKTVNISREEYEYPIPANEAAEIINKLADGVIEKKRYTITYKSKTWEVDE
                     FLGDNEGLIVAEIELDSEGEQFELPQWVGKEVSDDARYYNSNLLKSPFKLWKK"
     gene            complement(14393..14818)
                     /locus_tag="PK252_02145"
     CDS             complement(14393..14818)
                     /locus_tag="PK252_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT13537.1"
                     /translation="MYKHILVAYDGSEGAKTALNNSLAFAKAMKAKLTALWIGGYRAY
                     YHETVAEIDEESKAIDSFSAKLKKDLHNISKSEGIEIEYAHLQGNPAKLIVEYAEKNH
                     VDLIVMGCKGHSGLWGNDLGHVTDKVSENAKCNVLIARK"
     gene            complement(14821..16008)
                     /locus_tag="PK252_02150"
     CDS             complement(14821..16008)
                     /locus_tag="PK252_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT13538.1"
                     /translation="MDNIWLIAAMWMGMALVASLISIRVGISVALIEILVGVIAGNFL
                     GIHQTTQWIDFLAMLGSGVLTFLAGAEIDPASLKANLKASLAIGILSFVVPFASVWLI
                     AQYVFGWGIHQAQIAGIALSTTSVAVVYAVMIEKKLGDTSMGKMILAACFITDFGTVL
                     ALGVLFANFNLWMVAFVVVMCVMLWFMPRWTKYLIGHLGTNKVSQPEVKFILLVLFFL
                     GGLATTAKSEAVLPAYLIGLVVAGVFVRDKTLVDRMRTIAFTIFTPFYFMKAGLYVSL
                     PLIWVSLGIITLFLVIKIVTKFIGVYPLARSFQMRKKESSYTTLLMATGLTFGTISAL
                     FGLNNHLIDQKQYTILVTVVILSAIIPTLIAQKFFQPEIHTMIAWGRLRRMSKKNITK
                     RRK"
     gene            complement(16021..16389)
                     /locus_tag="PK252_02155"
     CDS             complement(16021..16389)
                     /locus_tag="PK252_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOT13539.1"
                     /translation="MEIKGEAKLLRIFISNTDKFKHNLLYEMVVFAAKRYGLAGATVL
                     KGVMGYGSSSVVRSIKFWEFTEKLPVVIEIVDESEKIEKFAEKILPWFEKLRYGCMIT
                     MEKANIVLYKQGEKKSFFKW"
     gene            complement(16392..16769)
                     /gene="crcB"
                     /locus_tag="PK252_02160"
     CDS             complement(16392..16769)
                     /gene="crcB"
                     /locus_tag="PK252_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOT13540.1"
                     /translation="MIKILLFIGSGSFLGGISRFLTSRFIQNTFIASFPYGTFVVNIL
                     GCFLIGLFYGISERGNAMSSELRMFLTVGFCGGFTTFSTFANENVALLRDGNIFYFAL
                     YTSLSVFIGILATYLGNLITKII"
     regulatory      complement(16801..16864)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16879..17364)
                     /locus_tag="PK252_02165"
     CDS             complement(16879..17364)
                     /locus_tag="PK252_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT13541.1"
                     /translation="MKRLRLTSKTKQALSAAFMVLSVLFFVLLFVFKDTLNNYASKAI
                     KTQAGPEVQHSVPAFVDSAYNYAKNGLSYQFTFLEFGAKGCSACKRMEQVMAEIKARF
                     PDRVNVIFINIMLPENQRLMKYFGIAAISSQVLLDKEGKEFFRHTGYYSTNELLIAFR
                     F"
     gene            complement(17339..17716)
                     /locus_tag="PK252_02170"
     CDS             complement(17339..17716)
                     /locus_tag="PK252_02170"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOT13542.1"
                     /translation="METNNNVKTCLCGSGEYVVIACSGACDLGQISDLVARKLRDNNI
                     RKMNCLAAVAADVKPTIEMFKEADLLLIDGCPVDCGKKILDKAGITNYKYMRMTDLGY
                     VKGQTPVTSEVIEAVYEKAAVDF"
     gene            complement(17721..18806)
                     /locus_tag="PK252_02175"
     CDS             complement(17721..18806)
                     /locus_tag="PK252_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13543.1"
                     /translation="MKTKLRFLLLFTCICYSVQIFGQDTDTTFKPSGKPIVQVFGYSK
                     LDATKDAKQPVSFGITRAHFGYNYNFSNSFSGTLIIDAAGRPTTVGNIVVTDSTGKNY
                     TVNNTSKEGSYYTAFLKFAFIQWKPSEMFTLQVGGILQNHYITQEKFWGYRYVYETFQ
                     DRYYGTASGDFGAIGFVKVNNILAFDVAVTNGEGIRFKQDDYGKVKYATGIDIKPLKG
                     LLTRLYYDNTQSGDPSDNATQHLFSFFTGYQLDKVFRAGLDVNYRLNNLNYNDHDFWG
                     YSLFGTYIVNPKIEIFARYDNVLSNTLSGQSQSWNIAKEGKAYIMGIHYQPVKTVNLS
                     INYQGWKAKQSDKKHTNIIMLSTEFKL"
     gene            complement(18807..19577)
                     /locus_tag="PK252_02180"
     CDS             complement(18807..19577)
                     /locus_tag="PK252_02180"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOT13544.1"
                     /translation="MKTISFIGGGRITRILAQAFNNANVRFDKVVVYDVSEEALKAVK
                     AKFPQVTTTTSVDEAATAELVILSVHPPMVMETIEKIKGSLQPGSVLLSLAPKFTIEK
                     LTALLGGFANIARMNPSASTIVNKGVNPVSFSASFDADKKKAFLELFRPLGYEPEVAD
                     NKIEAYAVISAMGHTYFNFQLQKLKELAVSFGMDEQEAAKTISNMLWGTTETLFNSGL
                     PYAEVADLVPVKPMAEVEETIKGYYDQYLTGIYNRIKP"
     gene            complement(19603..19803)
                     /locus_tag="PK252_02185"
     CDS             complement(19603..19803)
                     /locus_tag="PK252_02185"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HOT13545.1"
                     /translation="MKVTLIIMVLVMTFVVTFVSVSVNFGYHAGFIAKWMKSWGLAFV
                     AALPVVMVIMPIIKKTIAKYVE"
     gene            complement(19841..21181)
                     /locus_tag="PK252_02190"
     CDS             complement(19841..21181)
                     /locus_tag="PK252_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT13546.1"
                     /translation="METKKEFKILLWIAVIFGAAFFLPIESVRFNTAIAATFDLVKWY
                     AREHVILCLLPAFFIAGVISIFVSQGSVLRYFGANAKKWLAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARTIGAVSFSII
                     IGVAMSLIYRKEEKAKREEQMNIEFQAEKRPMWQTSFHFFTLVLILVFANWGSPASSD
                     TSSAWYYIWAYKWYITGFFALLLGYSLIYILKIKWQWVLLATVATVTSAFLSSTFIEN
                     VKLAPLVPMIVGIAALSTITLLDKKDEDNKEWTISAWGFAKQILPLLAIGVVTAGFLL
                     GSTHDGKTIAGIIPNEWISALVGGNSVFSNFFASVVGAFMYFATLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVMGTQKTVVYVTLVIVMATISGLIFGAI"
     gene            complement(21240..21938)
                     /locus_tag="PK252_02195"
     CDS             complement(21240..21938)
                     /locus_tag="PK252_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOT13547.1"
                     /translation="MDFFNSILENSTMPWLSALVLGLMTAISPCPLATNITAIGFISK
                     DIENRHRVFINGLLYTLGRAISYTAIALVIFLGVDQFKFSGIFQQYGEKFLGPLLIII
                     GLFMLDVIKMKFPGMGKLTSRMENKATWNYIDTILLGIVFALAFCPYSGVLYFGMLVP
                     MTVASASGLYLPVIFALATGIPVILFAWVLAYTVSGIGHVYHKVKTFEKWFRRIIAIL
                     FILVGIYYIIRVYL"
     gene            complement(21945..22352)
                     /locus_tag="PK252_02200"
     CDS             complement(21945..22352)
                     /locus_tag="PK252_02200"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOT13548.1"
                     /translation="MKQIVMFSFALVMTLGSLSCNAQTTKKEDKPNASAKVEAYYFHF
                     TSRCVTCKTVEAEAKESIAALYGDKVSFQSVNLDEASSKALAEKLKVSGQTLLIVKGD
                     TKINITNEGFMYARSNPEKLRSVIKEKVDELLK"
     gene            complement(22457..22696)
                     /locus_tag="PK252_02205"
     CDS             complement(22457..22696)
                     /locus_tag="PK252_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT13549.1"
                     /translation="MEIKILGSGCAKCKTLEKITREVVEQNGINANITKVEDIVEIMK
                     YNIMATPALVVNEKVEIKGRVPSADEIKQVLLKAI"
     gene            complement(22786..23100)
                     /locus_tag="PK252_02210"
     CDS             complement(22786..23100)
                     /locus_tag="PK252_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT13550.1"
                     /translation="MSEKEKYYTDSQEKVARFAKALSHPVRVFILDYLANNIDKCCYS
                     GDMAEELPIARSTLSEHLAELKKAGLIQGEINPPYIKYCINRENWEEAKKTMIQFFNL
                     KI"
     gene            complement(23184..25481)
                     /gene="gshAB"
                     /locus_tag="PK252_02215"
     CDS             complement(23184..25481)
                     /gene="gshAB"
                     /locus_tag="PK252_02215"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HOT13551.1"
                     /translation="MNNDIFTPLVSQIKGNIWTEGLFGLEKENIRVDKNGNLSLTPHP
                     KTFGNKRKHAYVTTDFSESQVEMITPPLPTIKETVGFLETIHDIVSLELEDEYLWPQS
                     IPPVLPDETLIPIARFDDEGKEAEQYREFLANKYGPKNQLFSGIHFNLSFGEKLMEML
                     YHKSNSVMTFEEFKDEAYLKVTRQMLRLRWLYVLLYGNSPVVDPSMELKCKQSPFTID
                     KHVIGLSIRNSCYGYQNIGELYPDYSSVQNFQQSIEQMVKDGKLASTKELYSSIRPKF
                     IKDPKHITYVELRFIDIDPLTKAGISEEALHFLHAMAIYGLLSEEPDSFDATAQSHAN
                     QYHGYVSLYGLIPNPHANQEEADVWAKAKKHIQHMANLLQDLMVNNESIIKSIDVANK
                     LIKNPKKRKVYEVLDGILQKGYVPFHLEKAKSYLNASQQKSYNFIGLEDMELSTQLLL
                     REAVKRGILFDILDRKENFVRLKQNGNIQYVKQATKTSLDNYASILAMENKQVTKKIV
                     REAGIRVPEGYEYTNAEAAKTDFALFKGKAVVIKPNQTNFGIGITILKENTDETTFFR
                     AVDIAFEHDTSILIEEFIEGKEYRIFVINNEVAGILHRVPANVVGDGNKTIRELVEIK
                     NQDLLRGKGYRTPLEKIQLGEAEAMFLKSQHKTFDDVPANGEVVYLRENSNISTGGDS
                     IDFTDEIPDSYKQIAIKAASALNVKITGLDMMIKDYYQEARPDNYAILELNFNPAIHI
                     HCHPYKGKNRKLNEKLMDALGFKTI"
     gene            complement(25595..26959)
                     /locus_tag="PK252_02220"
     CDS             complement(25595..26959)
                     /locus_tag="PK252_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT13552.1"
                     /translation="MKYLHFSGILNKCQASSTMKQLICLALPVIATSFMSMAYHFVNI
                     IFVGQLGSSAVAAVGSAGFYMNLGWGLSSLLTVGAGIKVSHSIGQKNNLLAKSYVKSG
                     IVATLIVAVLYYLVLLATKSFLVGIIRLNDPEIEQATTNYLMLTGLSIPFTFQNLFFT
                     NVFIGSGDSKTPFRINALAFGINIALDPLLIFLAGWGIYGAAVATILSQAVATIVFYN
                     KLYKNEMLRPKGVTFRKVLLKNIIGLGISPTIQRVSFTIVAIAMARIISNWGANAIAV
                     QKVGIQIEAISYMTIIGFMSALSSLSGKAYGAKNYQQQWETFRSGMVLAFIIGIITTA
                     ILIAFPSSLFSIFLHEKESVAMGREYLLILGVSQLFMCMELMATGAFFGWGRTNIPAI
                     TGITLTVLRIPMAIAFIHFWENSLSSVWWSISISSIAKGSLLVLLYVILFKIFIKNTQ
                     SVKS"
     gene            complement(27035..27934)
                     /locus_tag="PK252_02225"
     CDS             complement(27035..27934)
                     /locus_tag="PK252_02225"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOT13553.1"
                     /translation="MIYENILETIGNTPLVRINKLNPYENISISAKIEGFNPSGSIKD
                     RIALKMIEQAEAEGSLVKGKTIIEPTSGNTGIGLAMIGRMKGYDVEIVISKGVSIERV
                     KMIKAFGAKVTLTDEKLGTDGAIIKAKELVKAHPEKYFMPDQFSNSYNKIAHYKTTGE
                     EIWKQTNGKITHFVSALGTSGTIMGVGLALKENNPNIKIVCAHPVKGHYIQGLKNMEE
                     AIVPAIYDPSQIDETIMVETEAAYDMARQIIRKEGIFVGMSSGAAMYAALEVARKIDS
                     GNIVVIFPDSGEKYLSTGLFKEN"
     gene            complement(27945..28412)
                     /locus_tag="PK252_02230"
     CDS             complement(27945..28412)
                     /locus_tag="PK252_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT13554.1"
                     /translation="MKNTENQTIKKFRELLRRFEREIFVQTNECCCSNVTLAQCHTLL
                     EIESKEKETLTELANNLGLDKSTISRTVDGLVNIGLIQRTIPAEDRRTSILQLTQAGI
                     DVCHTINGNNDKYIEKTLSVLSDQEKDELIRLFEKITSQMVKLRSTGCCPIND"
     gene            28552..28974
                     /locus_tag="PK252_02235"
     CDS             28552..28974
                     /locus_tag="PK252_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT13555.1"
                     /translation="MKLNSISSALNEKGLKVTPQRIAILEAVFKLCNHPTAEQVLDYI
                     RKKNPNISLATVYKVLDAFVENGLVKTVKTDKDIKRYDACVEKHHHIYYSESDIIEDY
                     MDAELNDLLEKYFERKQITGFKIEEIKLQIVGKSTSKY"
     gene            28977..29360
                     /locus_tag="PK252_02240"
     CDS             28977..29360
                     /locus_tag="PK252_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOT13556.1"
                     /translation="MNVFRFVLIVLGTLSLALGIIGIFVPGLPTTPFILLTAGLYMRS
                     SDWLYKWVINNRFVGSYVQNFQKSNSIPLQTKLYSIAMMWTMIGISTFFFIESLWGKG
                     LVVLAGVVGTVVVGIVIKTRKHSNL"
     gene            29491..30006
                     /locus_tag="PK252_02245"
     CDS             29491..30006
                     /locus_tag="PK252_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOT13557.1"
                     /translation="MKISKKIEEVLNKQVNAELWSAYMYLSMSAWAENKGFKGFANWM
                     RVQFHEETSHALKILDFILTRSGEVKLEPIAAVKTSWDSLLSMMEETYEHECKVTSLI
                     NQCYEVALAEKDYATATMLQWFIEEQIEEEANALEIIDVLKITGEKSGGVFYLDKKLG
                     HRTFSDDTKKE"
     gene            complement(30197..31339)
                     /locus_tag="PK252_02250"
     CDS             complement(30197..31339)
                     /locus_tag="PK252_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT13558.1"
                     /translation="MNYLVREITENLLNKLQSNKVVIVYGARRVGKTMLVKKILEKVN
                     EPVLRLNGEDINVHDKLAIRTIENYRQLLGSYKFLFIDEAQKIPDIGVKLKLMIDEIE
                     GLKIIISGSSSFDINREAGEPLTGRKYTFNLYALSEREYSQIEDRISKTDKLRERLVL
                     GNYPELLHLPDKKDKIEYLNEMVSSYLLKDILVYENIKNSQTIFNLLRLIAFQLGKEV
                     SLQELGQQLSISKNTVEKYLDLLSKVYILHKVEGFSRNLRKEITKSSRWYFLDNGIRN
                     AVIANFNPIESRNDIGQLWENYMISERIKYQEYKRISSNNYFWRTYEQQEIDWVEERD
                     GTLFGYELKWKESKVKIPTQWKNAYPDSSFEVVNINNFENWLEIKY"
     gene            31637..32362
                     /locus_tag="PK252_02255"
     CDS             31637..32362
                     /locus_tag="PK252_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13559.1"
                     /translation="MSSSNLYITQDTELSDIVRAYPGSMLFLEHFDIHVPFQTKTVDK
                     ICSEHNISSDLFIAFANLYLGKDSYQIPKLRFSDLPAIIRYLHNNHKYYLDEIYPKIQ
                     QCIKLIMGLNDLDESKLVEKFFQDYFNEVREHLNYENSVVFPFVTGLYDVFINKIPYR
                     QRNAYSVSEYKDHHSDIEEKLDDLMSLLIKYLPHKNDQQARRELFHLLVELDYDLKVH
                     ARIEDLILVPLVEKLEKQLNAKP"
     gene            32359..32964
                     /locus_tag="PK252_02260"
     CDS             32359..32964
                     /locus_tag="PK252_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT13560.1"
                     /translation="MSKVTNIIVVETSSVLYEGLVQVISHAGMPLHIRQAGNIDDAEK
                     FICAKPESLVIVNPAIIQHNVKSFQSLKNNWDKAHWMALVYLHYDQQVLGLFDGIISI
                     SDSPGTIISTIKKVLSSKHSQNTYISEDILSEREVEVLQLLAVGLSNKEIADKLSISI
                     NTVITHRKNISQKTGIKTVSGLTIYAVVNKYITLESVKPMV"
     gene            33585..34727
                     /locus_tag="PK252_02265"
     CDS             33585..34727
                     /locus_tag="PK252_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOT13561.1"
                     /translation="MKKIYTLALSLFFVTSIIAQTSENNSDDPLLLEASYTCDNVSNL
                     AGGIKTGYGYLGMATIRIGFDTEKAGLWKGGQLFVNAANTHGATPSADLLGDMQVASN
                     IEAGNHTYLQEFWLKQAVGHVELTVGLQDLNVEFANSEHGALFLNSSFGILPIISGNV
                     AAPIFPLTSLGLTAKWNITEKTSWLNAAYDGSPTDFDYNPHNIKWEFVSGDGLLAISE
                     LQQSLDIVGLPGTYKLGIYSHSHIVENNFNSTLADSLNYNTFGAYVYADQKVWAQAER
                     SLGFFVQAGYSPSKISVNNAYIGIGANLTGMLSKAKDDVLGLAVAHENFNNGLGSETT
                     IELTWQKQLFRNIFIQPDLQYIIKPSGKNSGLENCLAGIIRLGLAF"
     gene            35019..37484
                     /gene="feoB"
                     /locus_tag="PK252_02270"
     CDS             35019..37484
                     /gene="feoB"
                     /locus_tag="PK252_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT13562.1"
                     /translation="MQLSELTNNQQAVIAKVSGHGAFRKRITEMGFVRGKMVKVIKNA
                     PLQDPIEYEIMGYNVSLRRSEAALIEVVREEGAETIVQSGFEGTIVDDRLKISLKEKG
                     NTIHVALVGNPNSGKTTLFNHASGSHERVGNYGGVTVDAKEAYIAFEDYQLKVTDLPG
                     TYSITEYTPEELFVRNHIAEKMPDVVVNVIDASNLERNLFLTTQLIDMNIKVVIALNM
                     YDELEQKGISLDDDALGRMLGIPIIPTVAVKGQGIIALIAKIVEVYEDRDPVVRHIHI
                     NYGNTIEDSIARIQAELKKSKELTDRFSTRYLAIKLIENDKATLSQLKSFFNFENIEK
                     ISQAEIQKLEKEYHEKSETVIADAKYGFIAGALRETCSGCAENTTNKKWNVDFLLTHK
                     YWGFPIFFFFMWLMFQATFSLGSIPMDWIDAGIAFIGSQLGNLLPEGAVHDLLIDGII
                     GGVGGVIIFLPNILILFFFISLMEDTGYMARVSFIMDKLMHKIGLHGKSFIPLLMGFG
                     CNVPAVMATRTLENRKDRIMTMLITPFMSCSARLPVYVLIISAFFPKNQGLVLFSVYF
                     IGIILAVLIALLLKKTLFAKQDVPFVMELPPYRVPTLKNTSKHMWFKGSQYIHKMGTV
                     ILMASILIWALSYYPRNVQYAVNYDEQIEQITANATLADSVKESNIAQVELMKVGEHQ
                     EQSYIGRLGRFIEPAIQPLGFDWKIGISILTGLAAKEIVIGSMGVLYQADQEADENSG
                     SLIEKLQQQEHSSGELKGQKVFTPLVAFGFMLFVLIYFPCVAVIAAIKKESSWGWAVF
                     TMFYTTAIAWLVAFANYQIGSLF"
     gene            37609..37791
                     /locus_tag="PK252_02275"
     CDS             37609..37791
                     /locus_tag="PK252_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT13563.1"
                     /translation="MIQNIIVLMIVFTTVAYIIYAIGRSFKTKKTCNCDGCSGCDLKA
                     IVKAKELRTLKIVKNK"
     gene            37794..38429
                     /locus_tag="PK252_02280"
     CDS             37794..38429
                     /locus_tag="PK252_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOT13564.1"
                     /translation="MDKKYFKYALWLAVFTVAYNIIEGVVSVWLGAGDETLALLGFGV
                     DSFIEVVSGIGILQMILRIQNNPDKPRGRFEITALRITGWGFYVLAVGLFVGAALNIY
                     QGHKPETTFWGFVISAISIIVMLWLYKSKVKYGKKLNSEAIIADGRCTLVCIYMSVIL
                     LISSIVYEFTGFGWIDALGAIGLAWFSYSEGKEAMEKAKGKECCCCGHDNC"
     gene            38518..38934
                     /gene="arfB"
                     /locus_tag="PK252_02285"
     CDS             38518..38934
                     /gene="arfB"
                     /locus_tag="PK252_02285"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOT13565.1"
                     /translation="MPITEKTETEFEFRTSRSSGAGGQNVNKVETRVELWFNILQSTS
                     LTDNEKMLISERLSAKLTKDGWLRVVSQNERSQLANKKRAIAKFYHIMEQALWVDVTR
                     VATRPTRASKLKKKEAKIKHSSVKKARSKDWSKERE"
     gene            39037..41517
                     /gene="hrpB"
                     /locus_tag="PK252_02290"
     CDS             39037..41517
                     /gene="hrpB"
                     /locus_tag="PK252_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOT13566.1"
                     /translation="MSFNPFSIDLPITEVLKAVKDSLSKENTLIVSAPPGAGKSTLLP
                     LALLDEPWLEGRKIIMLEPRRLAARTIAARMASLLGEEVGQTVGYRIRFDNCTSEKTR
                     IEVITEGILSQMIISDNTLEGVGAVLFDEFHERSIFADVAMALCRETQNILRPDLRMV
                     VMSATLDIPNFQTVLKAPVIESKGKQFPVEIVYTGTHDLMMLPELAARTVAKAVSSHP
                     GDALVFLPGEAEIRKCEEILKGELRDFAIHPLYGQLTQSEQNSAIVPNKFGKRKIVLA
                     TSIAETSLTIQGIKIVVDSGFGRTSKFDPKTGLTRLETVAISKDSADQRAGRAGRLSA
                     GVCYRMWSKATQENMRGHRVPEIMEADLTSLVLDMAEFGIIDIQQLTWLTPPPKSAIS
                     QATELLHELEALEHGRITEQGRKMHRLPCHPRIAHLLLKAEETHLLALATDLAAVLEE
                     RDPLSRDAGIDINVRIETLRRQRSQQRIQRNFARIEQVASTYRRLFNIEADNDSHNPY
                     DTGFLLTFAYPERIACARPGNNAQFQLSNGAIAMAGHKDDLAHESWLAVAHLDARDGM
                     GKIFLAAPLNPKDLAPLVKEQEVITWDTRKGGLVATKDLKIGNIVLQSKPLPEPDEEH
                     KVLAISEAIKKEGEALLNFDDAFTNWQNRVLSLRKWRPQEAWPDVSTTTLLVTNYTWL
                     APHLSQVKKTEDLKKMNLVEILQAQLDWEKQAALDKLAPTRIEVPSGSKVAIQYQANG
                     DNPILAVRLQEVFGLADTPLINEGRTPLVLHLLSPGFKPVQITSDLRSFWNNAYFEVK
                     KDLRARYPKHSWPDDPWTAQALRGIKKK"
     gene            complement(41584..42363)
                     /locus_tag="PK252_02295"
     CDS             complement(41584..42363)
                     /locus_tag="PK252_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT13567.1"
                     /translation="MILRGTFFSGKLEIETNITFVAPDNFSGQPYKVVYLLHGICGRS
                     GDWVDYTLLPNYSKEYHAIFVMPEVARSFYSDMKYGLNYFSYITEELPLMCNSIFKIS
                     AKKEDIAIIGASMGGYGALKCTLSKPEQYGFCGAFSSPCLMLKESLEYCEGAKRFKEL
                     FGEALFKDFQAVFGENIEWDPREDVIELAKETKDKTTKPKLYITCGTEDFFYGDNKRY
                     FATLQSLGYDPVFEDWQGNHDWTFFNEALRRALKHFIEKEK"
     gene            complement(42889..46323)
                     /locus_tag="PK252_02300"
     CDS             complement(42889..46323)
                     /locus_tag="PK252_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT13568.1"
                     /translation="MKKLFIFLVAFFIATSFAQAAADINLGDFEDGTVNSWVTWGGTV
                     TNASNPLKNTDNNSDKVLQVVTTAQYQGVAKGGLNVNIADYQKVTLDVYDPTGAVNFI
                     VNVYGKNASNSDVSKGFYPSTNAGAWKHYEVDLTNSTNFGTFVSITQIALQNNTVATN
                     LLFDNIKIVAKPSTPPTKITLSAATLTPIVGKKITTSLTVEPATAGSQVTYGTSDATI
                     ATVTSAGVVTALKAGAVKIYATSTLDANVSDTLFFTISDPVVGTSMVQANFENGINPN
                     QTQGGWNGAGTPAPTVTIVDNPNKVGNSSDKVLKVEKCNRYAAAVKTPAIDLNKYNRI
                     KFKVYSDSAYVDFQFAMNINNSIAEKYTATGITKATWTEYEYVIDPAMAADGQFYIKM
                     NSPKATYSRVTPPNADSLGYTFYIDSITFIAGDGSTYKPVNALTIRGANNVNTITTDN
                     GTLQMEAVFTPEDASNKSVTWSLVNQNADTATISATGLLTAVRNATVKVKAVSVDGGF
                     SATQDIAISNQVVPVAGITITAPDTIRAANGPAKFFASISPADADTLTYISSLVDSTG
                     NAVTTASINAADSTITSLANGYAILKVVAAGNSTIKAEKTIIIVYQEVFVDSVKLTFD
                     NDTTITTRKGSIELKANVYPSYATNTNVTWSVSDATIATVSNKGVVKANGTKNGSFMV
                     YVTSNEGAKKDSIRITASNQVLFNLATFAGDLPAGATIDTIEGKKYLKVRVNGWNTTI
                     AVEPVVTYSNSTAKFTYKYDKDTVTKTDVVQAFIQFADSGFVSKKAFTENPASTTFKT
                     VTAKLQADTIVNLQIAAQSTANWSALKGYVVYISQMEFTNPFNMTTFEGKVPDGGEIV
                     KINNKDYLKIRVNGWGTAFAIEPVVAKSYSTVKFSYKYDKDTVTKTDAVQAFIQLAPG
                     DFKKNKAITDNPASTTIKELTSTLVDTTFSMLQLAAQSTANWSALKGYVVYIGKMEFF
                     TPVLVTKIEISGESSITTAKGTTTLTAKVTPTNATTTAVKWSVDNTAIATIDAKTGVL
                     TAVANGTVVVTATATDASGVKATFTVTITGQTSINNASIASLSVYPNPVVDVLNIRSN
                     ETISTVAIYSMSGQLNRIASVNGNSVELNLSGLKNGVYFVKIQTANGSTTQKVVKK"
     gene            47064..47522
                     /locus_tag="PK252_02305"
     CDS             47064..47522
                     /locus_tag="PK252_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13569.1"
                     /translation="MEIKYQLFEAENLLVQKYSGQFSMEEFAAYARFSGMNFASKPIK
                     KVLLDFRDLHMGNYEDFNETLDKVIKVREQVNENEFKNRAVTFVIWVEKPMPTAVAHL
                     FTANFSGKNYHYALTVDSIVDILKLPQHLRNVEKIVENAENTFYADNTKI"
     gene            complement(47592..48116)
                     /locus_tag="PK252_02310"
     CDS             complement(47592..48116)
                     /locus_tag="PK252_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13570.1"
                     /translation="MKRWVVILLFTFSLLSAQQEDKTTKGGFAIQVGNGVLYGGMGGM
                     IEYQLRFKDNVRITPYVGSGFSVGCSDSSSAEYVWYSYAIGVNLEMGRMHRFILGPQI
                     VGARNISNDPENAVIYKKSLDGLSFIAGYKGTAHFGLMWQVVFGVACLQDPLLQDDKY
                     FFQPHVGLGMGYKF"
     gene            complement(48373..48855)
                     /gene="ybaK"
                     /locus_tag="PK252_02315"
     CDS             complement(48373..48855)
                     /gene="ybaK"
                     /locus_tag="PK252_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOT13571.1"
                     /translation="MTKTNAARLLDVQGIKYDLVEYTVDESDLSAIAVASKLGQNIEQ
                     VFKTLVLQGDKTGVFVCIIPGGEELDLKKAAHVSGNKSAAMVPQKEILGITGYIRGGC
                     SPLGMKKKFPTYIDESCILQDYIFVSAGIRGLQIKITPNDLIASASCEVADLIMIGSH
                     "
     gene            complement(48852..49685)
                     /gene="nudC"
                     /locus_tag="PK252_02320"
     CDS             complement(48852..49685)
                     /gene="nudC"
                     /locus_tag="PK252_02320"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOT13572.1"
                     /translation="MINDIFPHQFNNQFIATKHIEESDYILHYSQNSLLLKQNGHGFE
                     LPRKKDVNEIYDTSASTFLFTYDNVPCFLLWDDPKKVNGQLSYQESNLFRDFPQKEIA
                     WVSIVGHQMKNWYLENRYCGKCGSKTIEKPDERAIMCPHCNHLVFPKISPAIIVAITC
                     EDKILMAKNANFRADWYSIIAGYADVGESLEQTVVREVKEEVGLDITNLRYYKSQPWP
                     VSGSMMIGFFAEADCNQPIIIDNKEITHAAWFTRGNLPNRPNNISIAGELIDLFEKGE
                     I"
     gene            complement(49842..50357)
                     /locus_tag="PK252_02325"
     CDS             complement(49842..50357)
                     /locus_tag="PK252_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOT13573.1"
                     /translation="MRTKNLIIITALAMFIGVNMVAQPNQENPPMKCEKAALCPFNNE
                     MGIPGLTTEQSAKLKELKLAHYKEVQPVKNQLKELKAKQHTLTTAEKPDMKAINANID
                     EITKLENTLMKARAAHIQQIRALLTDEQRMIFDSRKHKPGKKGMQIRHNRMEHAQMPM
                     KKCEMNVSENK"
     gene            50548..50769
                     /locus_tag="PK252_02330"
     CDS             50548..50769
                     /locus_tag="PK252_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13574.1"
                     /translation="MKTINWKKFIRKASVALLLITVVMLMASVLAMETMGKSHDLEEL
                     HEFFGFTFLILVMVHIVAFRKSLKNTFFV"
     gene            51167..51709
                     /locus_tag="PK252_02335"
     CDS             51167..51709
                     /locus_tag="PK252_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13575.1"
                     /translation="MKTLITLFITASLIGFAIYSCEKDKANTPQSTFKGQLISKSACK
                     SFLKSTRVITDITNTPDSLVCIGYSFDNSTNKLSIKHINAGFNCCQDTVYCNVSLSVD
                     TILIQEIEQNPKCDCDCLYDLNIEIQGVDAKKYQIKVIEPYVGDQKKILFEIDLTKDK
                     VGSFCVTRKQYPWGMKSLNN"
     gene            complement(51911..54121)
                     /locus_tag="PK252_02340"
     CDS             complement(51911..54121)
                     /locus_tag="PK252_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13576.1"
                     /translation="MRRLFYCQLLLSFFLIGLNNKNQAQLNPDTIDNFRKFTISNLKS
                     TKALGASSILQNAEFPMQFPTPDSTSEAVHPSIIDFQKEVNTSGTWGGYRYWMAYTPY
                     YNTNALVENPCIAASNDGKNWIIPTGFKFPLTGTPPTGNNCDVDIVYNPDVNEIWLYY
                     MYRGLIIVGKDSIYGAIKLIRIKENGESTEPETVKLYKPRDIATIVSPCFWREASNKW
                     LFWGVYILKPNRLVHCFSSDGIHWEEETLCKNSSNKYALDSSGFYSWHIACKPNYFEK
                     RIEFMIAARTSSYENVYNVACIIFAQCHMDTPNIIDLEFKTPILNNAINYRQWDTKLY
                     RPTFQSYRTDSGYYYKVWYSANHEKSWHIGYTDGKIPVSFSNKGLPQSVTFDSILYKS
                     VNDSAFDLKAFATSGLDVNFSIENANIAFNSGSKCILKGIPGITKVTARQEGNSIYKA
                     AKEVSRWLVVLNARQYYYQIVLDSLSDKSKILGVTNYSDNIAFSKYALAKSFYNNQFS
                     ISWDTKDKSLLLPDGNIKRFNWMHKDTCVDLIATVPLEDTVLCKGYKVLVKPESRLET
                     LQRLINSMTISALGIASNEDSTGIVSNLQLTSTLGEGTKIIWQSDDTSIVSNSGIVTR
                     PFNYSKNVTIKALVHIGPDTLSKEFNIVVKAKEIPTIAENVNKITIKPNPATTFITID
                     IADGKPHYVQIYDLTGKMVYEENFSGKTMVDVHSLNSGVYMINVSGIKKKFIKQ"
     gene            54276..54614
                     /locus_tag="PK252_02345"
     CDS             54276..54614
                     /locus_tag="PK252_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13577.1"
                     /translation="MELERKKRFTLAILLALSLVFSGILFYMYRSLKHDCSIDKSVLT
                     TEKSMLKQSNNELAAEKRALCDENFEQKEKIERMMETFKPEKKFTKGIYKFSIMKGTE
                     TVSTMQFQLK"
     gene            54687..55199
                     /locus_tag="PK252_02350"
     CDS             54687..55199
                     /locus_tag="PK252_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT13578.1"
                     /translation="MKKSITLILSLAFVSFYATSQSLYKIGHINTQELIELMPETDSA
                     QKKIDKLYKDLQQQLELMQTELKVKYQDYINKKNTFSESVLELKETELQDLNARIQKF
                     QTLAQDEVENKRNDLYKPILDKANAAISAVAKENSFTYIFDASGGALLYKADNSIDIM
                     PLVKQKLKLR"
     gene            55237..55653
                     /locus_tag="PK252_02355"
     CDS             55237..55653
                     /locus_tag="PK252_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13579.1"
                     /translation="MNTISLKLFFIEFQRNEITDHLIYCRLSNLTKDYSLKELLEKIA
                     DDEMKHYSFWKEYTNKDVKPYYFKLFWYGYIMPFVSKKLALRFMKRLENYAHLEYTAN
                     LLNEVEGQWIINDESLHEKMLNQVLENDYLSKTVEE"
     gene            55672..56370
                     /locus_tag="PK252_02360"
     CDS             55672..56370
                     /locus_tag="PK252_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT13580.1"
                     /translation="MTVGKKILIVEDEKRLADILKTGFDENGFAVEIAYDGYIGKEMF
                     SKNKYDLVILDINLPLINGYELCKAIRIVNNNVPIIMLTAMGAAENKLTGFEVGADDY
                     VTKPFNFKELLARVRVFLKRSSTIEVVNDEMIQIDDLIINTSKKTVFRGNTQIELTAK
                     EYSLLAYLAKNKGIVISRTDIAEKIWGLKFDTGTNYIDVYINYLRRKIDYGFSNKLIH
                     TRVGLGYVLKNENE"
     gene            56373..57746
                     /locus_tag="PK252_02365"
     CDS             56373..57746
                     /locus_tag="PK252_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT13581.1"
                     /translation="MNIRLKLSIQFSLIVISILLVFTTSIYYFSSSYRKNDYYNRIKD
                     RAYTTARLLLDVQEVDKSLLKIIDKNTQALYQEKILFYDQNNELVYSTTDDSVKINLP
                     LINEIRAAQTVETNDSLREVLGFLYKYNNQEYVVIASAYDIYGLAKIKNLKFILIVGF
                     LISIIITIIAGLIFSGRALKPMSKVINQVKKITITNLNSRVDEGNCKDEIAQLAITFN
                     QMLQRLEEAFILQRDFVSNAAHELRTPFTVMLAEVEYNLLQERTKAQYVNTLTNLAIE
                     IRKISKLSNGLLDLARISYDNSAFEINTIRIDELLLEICNDISDIKKEHHTNITFKSL
                     PEDDNDLNILGNAQLLSIAFKNIIENASKFSNYNVVDIDFYSLGKNILIQFKDKGIGI
                     SPDDIHKIFQPFYRGKNTQFIAGYGLGLALTLKIIQLHNGTIDITSELGVGTTVTVTL
                     PNKASFI"
     gene            complement(58207..58722)
                     /locus_tag="PK252_02370"
     CDS             complement(58207..58722)
                     /locus_tag="PK252_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13582.1"
                     /translation="MKTKIAIVALGATLIIPIMIACIYLFMVSCEKEKKSYLTNMNDS
                     LSINYGTSFGECYGYCHHEIELSGTTANITISSWHKDKYPTKQYTKYITTAKWDSIVN
                     EVKKINFAQLDTIYGCPDCCDGGAGWIIVKTRNDRHKVMFDLMTDGPAELKTLWKMLE
                     KINANIMSTHD"
     gene            complement(58840..59847)
                     /locus_tag="PK252_02375"
     CDS             complement(58840..59847)
                     /locus_tag="PK252_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT13583.1"
                     /translation="MADEKKLTPISEPLVKHIYTADPSAHVFEGKIYIYPSHDIDVDV
                     PEDDFGGHFCMEDYHVFSMDSPTDKEAKDHGVALHVKDVPWAERQMWAPDAAYKNGTY
                     YFYFPAKAKDGAFRIGVATSNNPAGPFKPQPEPIKGSFSIDPCAFVDDDGKAYLYFGG
                     IWGGQLQRWQTGKFVENVKTPFDLEPADDQPALCAKVARLTDDMLEFDEKPKDVVILD
                     ENGKPLLAGDHNRRYFEAPWMYKYNGKYYFTYSTGDTHFICYAIGDNPYGPFTYKGIL
                     LNPVIGWTNHHSVVEFKGKWYLYYHDSSLSNGETRLRCIKVVELKHRPDGTIETVTAY
                     K"
     gene            complement(60013..60714)
                     /gene="pgl"
                     /locus_tag="PK252_02380"
     CDS             complement(60013..60714)
                     /gene="pgl"
                     /locus_tag="PK252_02380"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT13584.1"
                     /translation="MGPTITTLRNTFEIASYIAHKLDKKLDAKKSTINIAISGGNTPK
                     EIFKHWSSHFKNEINWRNISFYWVDERCVPSSSSESNYGEAKRLFFDKVLTTQDNVFP
                     IVGDNKPHEEVFILNSLVQSKLPQKNNLPVFDLILLGMGTDGHTASIFPGQLELFNLD
                     EAYTTSVNPHTGQKRITLTGKTINNANEVCFIITGSDKKLIIDEIINKKGNWQSYPAS
                     FIINDNLEWIIEEIQ"
     gene            complement(60725..62251)
                     /gene="zwf"
                     /locus_tag="PK252_02385"
     CDS             complement(60725..62251)
                     /gene="zwf"
                     /locus_tag="PK252_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT13585.1"
                     /translation="MPENHILVIFGASGDLTWRKLMPALYELRHQEMLPQRFAVLGMG
                     RSALDDTSFRNKIKEGIRQFGRKEITRDDKIADFVENIYFLSADPQQFESFMLLKNRL
                     ASLDEMYGTKGNYIYYLSTPPALYGIIADNLNKAGMCGYCGTDGWKRLIVEKPFGYNL
                     ETAKALNKKLLDNFSEDQLYRIDHYLGKETAQNILVTRFFNGFFEPLWNRNYIDRVEI
                     TSAESVGVEGRGGYYDKSGALRDMLQNHLLQLVALTAMEPPTVITPTAIRNEFLKVFQ
                     SLRPMTEKDIMKNVIRGQYTASVIKGVSVPGYKEEPDVNPQSRTETYVAIKFFIDNWR
                     WGGVPFYIRTGKKLPTRVTEIVIHFKPTPHPHLLKEIEKTQAGNMLIIRIQPDEGILM
                     KFGMKEPGTGFNVKQANLEFKYASSDNSFVLGSYERLIHDCMIGDASLFSRGDAVEAA
                     WEFVDPILKAWETNCDIKLYGYPAGTWGPKVADELMGNNNTWRYPCKNLSDDGLYCEL
                     "
     gene            complement(62259..63155)
                     /gene="gnd"
                     /locus_tag="PK252_02390"
     CDS             complement(62259..63155)
                     /gene="gnd"
                     /locus_tag="PK252_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOT13586.1"
                     /translation="MKIGFIGLGKMGANMVQRLLIKGQEVVAWDPNADAVKLVESKGA
                     TGAKDIPSMIQALPEKKIVWLMVPSGKPVDDNLELLSTLVGKGAVIIDGGNSNWKDTQ
                     RRAAWLKEKGIEYVDSGTSGGVWGLQNGYCLMYGGSKEVCNSLTPIFDALTEPGGHLY
                     CGAIGSGHFTKMIHNGIEYGMMQAYAEGFEILEKSGFGTELNKVADLWMHGSVVRSWL
                     LELIGNALNEDPRLDKLKPYVADSGEARWTVDAAVELSVPAHVITASLFTRFASRQDD
                     SFAMKLVAAMRNQFGGHEVKQK"
     gene            63575..64744
                     /locus_tag="PK252_02395"
     CDS             63575..64744
                     /locus_tag="PK252_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT13587.1"
                     /translation="MLRMGWIKKCISFNEQLTVDKRIANQVVAILAVTALFSGILNIM
                     MGFPWWFTVLILGAIVLSVLYIALSVIKTKFVIPKAALIVALYVVVILLWLYNPGLSK
                     APVILSFAIIVFSIAIYVKNFVLLFLLNFIIVTSLVVIGVLFPPLFSFSYKNEIHRVV
                     DVLGSYFLLGMSVVLILRSIINSYIKAKNDSEQQKCQLEAANTQLQILNEKLRAINNE
                     LVDARAKLLENEEKLQTIFNNLHLLICIVDLKTSGFVNINPEFTDILGYSHEELLNRP
                     FYDFIHPDDRQKTAFVIEDVLLKGKMVISFTNRYQSKGGAYRWFNWNAFPALEKGIAY
                     AVAIDITDLMKTEQALKESNELLTLFIKNSPIYAFIKDVKKKKAGLFLPVTILLT"
     gene            64741..65709
                     /locus_tag="PK252_02400"
     CDS             64741..65709
                     /locus_tag="PK252_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT13588.1"
                     /translation="MIGVNGADMVGKTMEELFPFEFARKISMDDWMVVQSNQVINVDE
                     NLNDRNYTTFKYPILLGERNLLAGYTIEITERIKAEHLINEQNAELKKLNADKDRFIS
                     ILAHDLKNPFNALLGLSDLILKNIDTYNIDRTKSLIVYINQIAQNTYDLLNDLLAWSK
                     NQAGMLPFMPEKILLADICNEIIDNYKISFETKEIVAEYAGDCGIYVYADVFMLKTIV
                     RNLLANAIKFTNKGGHINIKTEIKDKEALITISDNGIGISSENMAKLWGQSCQFSTVG
                     TAKEEGTGFGLLLCKDFVEKHGGKIIVKSEVGKGSDFQFNIPLYIE"
     gene            65784..66245
                     /locus_tag="PK252_02405"
     CDS             65784..66245
                     /locus_tag="PK252_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13589.1"
                     /translation="MMEDNSQIIETVVAGEKKLELNEEALNELTQIRKWAMFFSVLGF
                     IFIALMVFAGLIMAALSSGLAGAGLGKLPMVFVGILYLALGIIYILPVIYLYRFSVDA
                     KIAIESKDAILIASALKSLKKHFRFIGVFTIVIFSIYVLAIIGFAIGRMIG"
     gene            66413..66733
                     /locus_tag="PK252_02410"
     CDS             66413..66733
                     /locus_tag="PK252_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13590.1"
                     /translation="MSFFAVVLLFFVQQYTASATIAENYNNPVTYTLKSAPIFIASGN
                     SFSSPTERVGHHLMALKKMPLFNLCFKPVDVFSNLITRFIFLQKSSLASVPSKLELHC
                     ILRI"
     gene            66924..67076
                     /locus_tag="PK252_02415"
     CDS             66924..67076
                     /locus_tag="PK252_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13591.1"
                     /translation="METNLNNNVENTNENEKPKGFWASGWAFLVFFAGLIVLVVAISY
                     LIKWFI"
     gene            67183..69591
                     /locus_tag="PK252_02420"
     CDS             67183..69591
                     /locus_tag="PK252_02420"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOT13592.1"
                     /translation="MMKVFWIFFFTFVFVLSIFAQEKKTLEALKISNPLTIDGILDEP
                     FYSQIAPAKDFVQLQPYNGKPSFQPTEVYIFYDQDAVYVGAMLYDSSPDSIFNYLSER
                     DNIGMSDYFGIYLDPYNQGQVAYGFFITPAGVQTDLKAVKSDYDYEDSNWNAVWESMT
                     RMTDKGWALELRIPYSALRFSGSVENVWGLNMFRNIRRYSSNNSWNFVDRNVNGFIHQ
                     QGQLIGINDIKPPVRLSLSPYVASYFERKKDGKVDFLYKGGMDLKYGISESVTLDMML
                     IPDFGQVQSDDKKLNLSPYEMYYDEKRQFFTEGTEMFQKGSVFYSRRIGASPKFNAED
                     MKESNEVVDFKPNETQLYNATKISGRSKNGFGIGFLNAVSVESRATMKDTISGETRDV
                     VVQPVTNYNVTVIDQSLKNNSYISLINTNVKMADNPFYANVTATEFNIRDKSKTFSLK
                     GKGGLSVRDNSSKEDGFFTNLGLEKNKGKWQYAVTQYIYSDKYNPNDLGYLQKNNQII
                     SEAWVYFQNIEPFGIVREYNWNLWYDYVRVFNPNTFCYHQGGGYMNVTFTNNYSFNFN
                     SALVSEQFNYDEPRVKGRFYHTPPYYYYNFNLNTDDRKLLSFYFHYGHTDEPFDQYFA
                     DFGDFQANMRIGQRASVYYGLGVNLVANVHGYVDHYANDDSIVFARRDVNTITNVLSC
                     SYVLNNKMSVNCRARHYWSGAKNKMFYLLMDDGSLAFNPSYTKNKDKNYNTFTVDMNF
                     RWIFAPGSELVFAWKSSSDVENEIVRMDYFDNLGNLWNDQANSLSLKVLYYIDYNSLK
                     RKNK"
     gene            complement(69629..70465)
                     /locus_tag="PK252_02425"
     CDS             complement(69629..70465)
                     /locus_tag="PK252_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT13593.1"
                     /translation="MEKITSLQNQRIKDVVRLQKHAERAKQKVFIVEGLKEISLALEE
                     GYILKTLFFNPNKGADIESLHISKNTEIIEITNEVFAKIAYREDSNGLLAIFHLKELY
                     LDDIKLSANPFILIAEQVEKPGNLGAILRTADAAGVDAVIVCDPKTDIFNPNAVRSSV
                     GTLFTNQVVACTNEQAYQWLKKKGIKSYAAALVDSSKPYTEFDYKKPTAIIVGSEAYG
                     LSQFWLTKADQNIIIPMHGRIDSLNVSVSAAIIAFEVTRQRNSPSLPCKGGERHLFSR
                     DV"
     gene            complement(70470..70895)
                     /locus_tag="PK252_02430"
     CDS             complement(70470..70895)
                     /locus_tag="PK252_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOT13594.1"
                     /translation="MKKANKSFKIIIDTNIWISFLIGKNLNGLQNHIENQNIRIITCI
                     EQLIELSEVFKKTKLRRFFKKDQIEEFFELLDESAECIEINTKTDLCRDPKDNYLISL
                     AIDSKADFLITGDKDILELSKVGNTIIAKFTEFNDIVSK"
     gene            complement(71214..72092)
                     /gene="fabD"
                     /locus_tag="PK252_02435"
     CDS             complement(71214..72092)
                     /gene="fabD"
                     /locus_tag="PK252_02435"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT13595.1"
                     /translation="MKKAYVFPGQGAQFIGMGKDLYDNSALAKELFEKANSILGFRIT
                     DIMFSGTDEELKQTKVTQPAIFLHSVILAKTLGESFQPDMVAGHSLGEFSALVANKTL
                     SFEDGLVLVSKRAMAMQKACEAEPSTMAAILGLEDAVVEEVCKSISDVVVAANYNSPG
                     QLVISGSVAGIDKAIEILTAKGAKRALKLPVGGAFHSPLMEPARQELAAAIEATPFAK
                     PICPVYQNVTASAVSDPSEIKKNLVAQLTAPVKWTQTVQQMIADGATDFVEVGPGKVL
                     QGLIKKINKDANVASA"
     gene            complement(72248..73441)
                     /locus_tag="PK252_02440"
     CDS             complement(72248..73441)
                     /locus_tag="PK252_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT13596.1"
                     /translation="MESLKRRSDWLVSTTKPPLTRYLMTRVEWDWRLIGVVGARGTGK
                     TTLVIQHLKEKYGLSDEAIYITLDDLYFSSHTLIDFVETFRSRGGKAVYIDEVHKYTG
                     WAREIKNIYDLFPDMKIVFTGSSIIDILKQDVDLSRRAVVYKLQGLSFREYLILNTNM
                     EFEPIQFKEILARHTEIAIDICQKIKPLAFWDEYLKTGYYPYFIENKNVYYLRLEQSL
                     NLTLETDLNFIDGFDAKNIPKIKQLLYVLAMSVPFKPNIVKLSEKIGIHRNTLINYLH
                     YLEKAQIIQTLFNAGVTPGILQKPDKIYLENTNISWLLSETQPDKGTLRETFFLNQLK
                     NAHTIKLPVKGDYLVDDKWTFEVGGKTKDNRQIKDLENAFIVSEDIEIGAYNKIPLWL
                     FGMLY"
     gene            complement(73619..76126)
                     /locus_tag="PK252_02445"
     CDS             complement(73619..76126)
                     /locus_tag="PK252_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT13597.1"
                     /translation="MLSSDNPQIDLAFKYLSSTKKNVFLTGKAGTGKTTFLRHLRNES
                     PKRMVVVAPTGVAAINAGGVTIHSFFQLPFGPWTPARQDITNGFITGSNERSFKFSQE
                     KLSIIRSLDLLVIDEISMVRADLLDAIDQALRQIRHSDEPFGNVQLLLIGDLQQLPPV
                     VKRDEWDLLRPYYNSPFFFDSLALQQTDYVCIELQHIYRQSDEVFINILNKIRDNNLD
                     KEALDHLSTRYIRGFEPDDNDGYITLTTHNEKANTLNWKKLNQIKLEPHIFTAKVEGE
                     FPDYAYPADFELTLKVGAQVMFIKNDISFEKRYFNGKIGIVISIDEDIIEVQCPNDQE
                     PIKVNRQKWQNFKYSINQETKEIYEEEIGLFTQFPLRLAWAITIHKSQGLTFDNVIID
                     AQDAFSHGQVYVALSRCRTLEGIVLSSPVNRGSLVTEYHISQYNRKIPSLSPTIDSFI
                     QAKKKYQIHLLQDLFDFKEIAKLLYPCIKISRENANILAGNLNLTFSQMLKSINDQFT
                     PVCDKFVVQLQQLSADTEDLETSETIQDRIKKGCQYFKDNIDQQIWEHCKNIQIETDN
                     KAVRNSLKSSFEKLCQKVIQKRKLLELTANNFSISKYLHNKARIMLDVEFGSKKVLED
                     SGNGFIKHSKLFGRLKKWRTEKAEESGRAETSILKTKILADIANKLPLCAKDLKDIDG
                     LTINKIAAFGGEILDIIYAFAKEQDISIKTTPDSFDKQLQSIKKPDSKKLTLEMFQSG
                     KSIEDIAKERSMAITTIEGHLAHFVGNGTLKIEALVEQPILNLIITYFKHARRKELTP
                     AKEKLGDAVSYGQLRMVLKHLEYTSEAIFEEDYEPPF"
     gene            complement(76147..76590)
                     /locus_tag="PK252_02450"
     CDS             complement(76147..76590)
                     /locus_tag="PK252_02450"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOT13598.1"
                     /translation="MFSQDFKEFIQLLNEKKVEYLVVGGYAVTIHGYPRYTGDLDIWI
                     NKTQENSILIIEVLKDFGFSSLNLTSDDFLKDGNVVQLGYPPLRIDILNDIDGVKFSE
                     CYTRKEYFNIDNIKIPFISTSDLILNKKASGRHKDIDDLENLISK"
     gene            76813..>78161
                     /locus_tag="PK252_02455"
     CDS             76813..>78161
                     /locus_tag="PK252_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT13599.1"
                     /translation="MASYLQIENLSKSYGEFVMFENFSFQLSKGQKVAIVARNGAGKT
                     TLFNILCGDDISDGGSVIFHKDITVGFLRQDPPFNPSLTVLEQVLDSSGKVKDAVQKY
                     GHAMASGNEELMHKAVEQMDALQAWDFEAKMKRILGQLKIFNLEQKMNQLSGGQRKRV
                     ALANVLLQNPDLLILDEPTNHLDLDMIEWLEEYLQTYCSTLLMVTHDRYFLDRVCNEI
                     LEIDNKQVFHYKGNYSYFLEKRAERLEIEQVNIDKARNLYRKELEWIRRMPQARGTKA
                     KYRVDAFEDVKEQAFKQKSNKEININFQGSRLGAKVIDAEHVSKKFGSLNIINDFSYK
                     FAAFEKIGVIGPNGTGKTTLLNLLTGSLKPDNGIVEHGETLKIGYYRQDGLNFNEQMK
                     VIDVAQEVAEVVTMRDGNQLNVSQLLNMFLFPPPVQHSFIYKLSGGEKRRLYLMTIL"
BASE COUNT        22832 a        14855 c        15335 g        25139 t
ORIGIN      
        1 ccatagctat acaacgtgcc agccctatga ggataactcc gtacatatag tcgatgttga
       61 attgaagaaa tacaatggcc aacaggaaca tcagtattgg cccaataacc cagttttgga
      121 ctaacgaaag gccaagtatc ttggtgtttt tgaatacatc tcctaattct tcatatttta
      181 ctttggccag cggtgggtac atcatcaaga taaggcctat ggcaatagga atattggttg
      241 tgccagagga aaagtggttc caaaattgag caatgtcagg atttagccaa cccataagga
      301 ctccggcaaa catggcaagg aaaatccaaa gggtcaggta gcggtcgaga atttttagtt
      361 ttactttcat ggattgtata atttgaagat gaaatgattt gaaaatttga aaattaggtt
      421 atgattttga ggagctgatt attttagaaa ctacattaat gatagattgt aaggattctt
      481 ttaacccgtt gggacttgga taattttttg aaagttcgca aagagtaagc caatattcag
      541 tttcatcggc ttctttagcg gcaattttca ttttatgtat gaaatcagct ttactttcag
      601 cattctgagc ttctcgcaca ttagcaccta ttgaagttcc tgattttaat aattgtttag
      661 aaatgatata ttttttatgg tcttccaaaa tctcagcata ttcaattatc tctaaagcaa
      721 actctaatgt tagggatact attaaattct ctttatcatt tctcataatt ataggcctct
      781 aatgatttga agatgaacca atttgaaaat ttgaagatgc aattcaattt ttaaatcttc
      841 aaatcttctc atttccaaat tttcaaattc tcttgataaa acttataaaa cccttctttt
      901 atctcatctc tgacacgtct aaactcgctc cagataaact cttcggtacc tgtggcgtgc
      961 gatgggtcgt caaaaccaat atgaaggcgg tgttttactt ttcctaaaaa tgcagggcag
     1021 gcctcgttgg ctccaccgca aacggtaatg acgtaatccc attcttcatt gagatattta
     1081 tctaccgagt cagaagtgtg atgactgatg tccactccaa cttctttcat tactgcaacg
     1141 gctttttcat tgagcttacc ggaggcttgt gttccggcag agcatactgt gaggcttttg
     1201 tcaaatgatt gtaaaaagcc atgtgccatt tggctgcggc aactgtttcc ggtacaaagg
     1261 attaatattt tcatactatt taatttgaaa atttaacgat ttgaagattt gaaaatgaaa
     1321 tgttttgctg tatttattta gttttaatgc attcaaaatt ttgtttgctt atggggccga
     1381 aaaaattatc gaatagtttt agatattttt caatatttga actacacaag caataattga
     1441 cctttaggcc atctatttcg ccatgtataa ggcctaagtc gcggagctct tttaaatgtt
     1501 gcgatactgt gctcctgctc aaaggaatgc actctgatat atcgcccgag atgcatgttt
     1561 ttgtttcggc caaatatttc aggatggcta accttgcggg gtgcgatatg gctttggcaa
     1621 accgggccag ttctttaaag tcattatcaa aaatttcggc tttgttcatt cttaattatt
     1681 tttaatgacg taaacatacg tcatatttga ttataaaata aaattttacg tattaattta
     1741 ttgttaagct tttaatttgt ttttattgtt aagcttttaa tttctttcag ttgctcaata
     1801 aacctctgca actcgatatg attgtttttg tcgtttaaca ggatatgttc tacatctttg
     1861 ttttcgttga taataaacat taaagcctgt ttgtggatgg catcaataga tttgatgatt
     1921 ttttcagtca atcccgatat gtaacggtgc aggtttttgt ctgtttcgtt gggtatttgt
     1981 ttttcaaagt gatgataaaa cagcctttta aaccatctca taggtaagaa aaacaacaag
     2041 gtgtctaagt gcgaatcaaa agcacgataa acagacacat cgggcttgtc tatgcccgaa
     2101 aactcgattt gccaggatac ctcgggcagg ttgacaccaa acatctggtt aatgcggtca
     2161 ttgagccttt gtctatattg tagggctgaa tactcgtaat agctggcaat ttcttttact
     2221 atttggttga tctgttcaaa acaggcattg tcgatggttg ttatttgatg gccaaggttg
     2281 gccttgagcc attgttcaaa actacgcgaa acattgaaca atgagccatt ccaggtttga
     2341 tatgcggcat aaaactgttc ttccaactgt tgggcaactt tagcctggtt gggcagtact
     2401 attttttcga gcttgtccct tgtttcgcct ttaaacgaag agcccgaaag gagcatttca
     2461 cgctcgtggt ggtgacggtt ggccttaatc tctttaagca ccttattgat ggcttctttt
     2521 tcattttcac gtttcaaggc agtttgcaat gccagttcgg cataacggat gctttggctg
     2581 atggcgtttt gtatcttgta acggattatt tcgtcgtact ctttttcata atccttgtta
     2641 aaagggttta taaagttatt tatcaaagtg tccctgaata ttgtcaactg gctaaaaaca
     2701 gaatattcaa acatggggat atcgcggctt attgcttttt ggagcgagaa gcggatatgg
     2761 gcctttattt cttcaatttc attagtttta aacaaatcgg ttttggtgat taccagcgca
     2821 atattagggc aatgcagcat aacccctttt atgaggttta aatcttcttc ggccaacggg
     2881 cgttcggcgc ttacgctgat aatagcaacc ccggtgaatg gcaaccactg aagcgttgct
     2941 tcactattgt gcctgtaaaa gctgcttaac ccgggagtgt caacaatgct gatattctta
     3001 aatggctcta atattgggtg ttctacaatg gcaagtgaaa catttcggtt attctcgggg
     3061 ttatgttttt cagtaacgta taaaggcagt tcgttcaatg tggtggtaag atcttcttta
     3121 tcagtaaacc tgatggtcag ttttggttca gggccatatt gcaagcgggt tacaatggct
     3181 gtaacaggca caacgccaac aggcaggatg ttttcgccta taatgttgtt tactaatgag
     3241 cttttgcccg acttaaactg tcctaacact gctatgtgga tggctttttt gttttcggcc
     3301 aacaggctct caacaggttt tgtaagctca ggtgcacccg ttgaatggtt tatttgggct
     3361 atttcgcgta ttatgttttt gtaattgtcc atgttgcaat ggttttataa aataatgaca
     3421 attgaaccag ctataataag gattgtgcct aaaaggattt tccaagtaat cacttcgtgt
     3481 aacagtaacg acgaaagaat taaagcaatg gcaacgctca atttatctac aggtgccacc
     3541 tgcgatactt tgcccaactg taatgcctta aaataaaata tccatgataa acctgtggca
     3601 atgccagaga gtccaagaaa taaccaattg cttttggaca gggcatttat tccataaatc
     3661 tcttttcggg caagtacaat cccccatgcg ataaacaata taaacaccgt tcgtatggca
     3721 gtggccaaat ctgaattgat ccctttaatg ccaactttgg caagtatagc cgtgaaagcg
     3781 gcaaaaaaag ctgataataa agcataaacc caccacataa ttataattta aaaaaaaatc
     3841 atttgtaatc ctgataagac taaaaggcta tcgaccaata ataaaaactt ctcgcggctc
     3901 aaaaacgcta tcattttttt acccgtgtaa ctgcctaaaa tcattgccgt gccacaaacc
     3961 aacccataac caagttcgtt aaacccaata agtgaatact tctggtaaac tacagttttt
     4021 gaaatatgca ttaataaagc tgtaaaggcc tcacttgaaa tataagctat tggtgtaagg
     4081 tttaaactta caaaacaagc agcccccaat ggcccggcac ttccagcaat acctgacaaa
     4141 aagcctgttg caataccccc gacaaacagc cacctgtttt tggcagtaaa aaactttatc
     4201 cccaaacggc gataaattac catcatcaac aataaaaagc ctattccctt tattaaataa
     4261 actgttgaag aaacaaccag tatcctgccc ccgataatgc taaaaggtaa agctccagcc
     4321 aaaaaataag caattggttg ccattgaagt tctttgcggc caaaccatac ccgcgaggca
     4381 ttcccgaata actgggctat ggtaagcaca ggaattgcag ctttgatgcc aatgaaatga
     4441 gttaaaaccg gcaaaagcaa caatgcccct ccaaaacccg ctatacccga tatcgaagct
     4501 gcaagccaac taccaatgaa taatataaaa agtatgaata acattaattc gattgtttgc
     4561 ccattttttt atcaaactgt aaataaataa acgcaaaaat aaacccgtaa caagccccaa
     4621 tgatggctgc cacaatgaca tcgcccggat aatgtacccc attgtaaata cggctgtacg
     4681 aaaccaatac agcccaggca aaaagccaat atttcaaata cttaaacgta ttgtcgaggg
     4741 caaaaaacaa aaatgtggcc aacccaaaag cattggcagc atgagacgaa acgaaaccat
     4801 acattccgcc agcccctgct ttgctcaaat gtaccaagcc tgccaaagaa ggctcgtgcg
     4861 atggccgcaa ccgctgaacg gcatttttta tcaggtgcga agcggtttgg tcgcacaatg
     4921 caattactac acccaccaac aaaagcatta caactccttt cattttgtaa tgcttcacaa
     4981 taaaaaaggc tattaataag tacatgggta tccatatcag tttatcgcta aaccagtaca
     5041 tgattgggtc aaaaaaggca ttgtgtttgc cattgagaaa taaaaacaac tgagtgtcta
     5101 atgaatttaa ggactgtatc attattttat ttttttatat aacaagtgtt tttctttata
     5161 ccatattgtt gctgtaatta tgccaataaa tgctccaacc agcacatcgc tcggatagtg
     5221 aacgcccaaa cacatgcggg agtatgctac agctattgcc caaatatacg aaggaataat
     5281 aatataccac tttggatatg ccaaactaac agccgttgct atagcaaaag catcggtagt
     5341 atgccccgat ggaaaagacg ggctgccgcc actggtaact ttttcgagaa aaggatacgt
     5401 aataaacgga cgggggcgat ggatactata tttcaaaata gttgcaataa ttgctgtcgt
     5461 tgtaactgaa actgccacat ataccgaacg ctggcgggca tcaatgtttt ttctgcggaa
     5521 agcaattact aataacaaaa ctgccaatcc ataagccaca gttgatgcgg agtcggtaat
     5581 gaccctgaaa acaccgtcga gatttttatt gcggttcagg ttgatatctt tcaatatgtc
     5641 gatatcaata ttttgggagt atagtactat tggagaaagt agcaataaaa atataagata
     5701 tttcttcatt tgtatgaaat tttaatttat tgaaatttaa ttatttaagc cattaatatt
     5761 tgttatttaa agtgagtgga tgcaaccaac catccatgcc gatttatcgg gattcatgtg
     5821 tgtttttttt gggggccgca atcatgtgtg tttcatttta attcaaattt ttagaagata
     5881 gtttaacgaa taacggaaca gatgccagtt gcattactac cgagaacgca attagccata
     5941 taatagacat atcatatagc cagcccatta aaaagcttcc cagaaaccag aataagccaa
     6001 aaactgcatt aaaaacccca aacccggttg cttttttatc ggcgggcaat atggatgaca
     6061 ggacagccct tacgacagat tcctgtgcag ccatgcccac gccccagcaa accattccgg
     6121 ctatggctgt gtaaaaattg cccataaata ctaacggggc aaacaataaa gatagcaatg
     6181 tactgccaat aagtactttt ataccatgtt tatcaaacaa cctgcccagc aacaaagcgg
     6241 ccaatgcatc gacacccatt gcaatggcat acattacagg aatccattgg gtagtaacct
     6301 gtccggtttt gttaaagtgg aatgctataa gcggataatc tgcaaacccc gcagcaataa
     6361 gcgatacggc tgccagataa atccagtaaa acttttttgg aaaaacaagt tttggcatgt
     6421 tgtttttatc ttccaaatgt tcggtccttg ggtagtttag ccttgcaaca accaatattg
     6481 ttaaggctat taaggccggg ataagtaata tcccgaatga tttagcgtaa ttgcccgaaa
     6541 agaaaaacac agcgccaacg ataattggcc ctgtaactgc ccctatctgg tcgagcgctt
     6601 catgcacgcc aaaagcctta ccatgaccta ttgaagacga ggcatgagaa agcatcacat
     6661 ctcttgcagg gttacggata gcccttccgg tgcgttccag aatcattaac acggctgcta
     6721 tctgccaatt tccggcaagg gcaagtgccg gtactgctaa aaggttgatg gcataaccga
     6781 caaatgttat agtccagtat tgctttgttt tgtcagcaat aataccagag gctaaccgaa
     6841 tggtataacc catgagttcg ccaaaaccgg caataaaccc gacaactgtt gcgcttgccc
     6901 ctaatatgcc cagaaactgg cctgtaatag cccgtgcccc ttcatgggtc atatcagaaa
     6961 acaggctgac aatgccaata agtaatacaa aagcccaggg agtttttttt ataaacttca
     7021 ttttctttac ttcattttgt tatacaataa aaaatgctgg tttaattgga agcaaagtat
     7081 tttgccccaa aattatggtt gaccgaagat gctcatggag catgatgaag aaaaacataa
     7141 aaccatcaag ctgacaagta tgcaatacaa taacagcagg tgcctttatg accctgtgaa
     7201 ggctcttggc cttgtaagtg aacgaaaata aaatgacaaa attatcaaca atagatatca
     7261 taaatgactg gttttaataa aattaaaact acaggagtca tcagtcattt acgacaattc
     7321 aaggtgaacc ccatcaccac acaagacaaa ggtacaaatt tataattttg aaaaatggat
     7381 gttgttgtta aaaagagcta aaaatgcgcg gaaattttca ctgaagcttc cgcgccgtag
     7441 attttacctg tttttaaaag ctgcaatgcc tgcaaatttt gagtttttcc caagttcatg
     7501 ttcaatgcgc atgagttggt tgaatttttc aatacgctcg ccacggcaac cgctgccagt
     7561 cttgagatgt ccggcattga cagcaactgt caggtctgca ataaacgtat cgggggtttc
     7621 gccgctgcgg tgcgatacaa agcaattgta actgtttttg tacgccagtt caatagtttc
     7681 aagcgtttct gttactgtcc caatctggtt cagttttata agaatagaat tggctacacc
     7741 tttttcaatg ccttcagcta atattgcctt gttagtacaa aacaggtcat cgcctactat
     7801 ctcaattttg tttccaagtg ctttggtcag atttttccat ccatcccagt cgttttcggc
     7861 caggccgtct tcaagtagca caataggata ttggtttgtc cagctttccc aaagttttat
     7921 aagctcatcg cccgtaatta atttgcctgt acttttgaac attttgtatt tgccatcttc
     7981 ccaaagttcg ctggcagcag ggtctataca tatactaact tcatttccgg gcttatatcc
     8041 ggccttattg atagcttcga ggatgacttc tacagcttct tcattcgatt tgaggtcagg
     8101 ggcaaaacca ccttcatcac caacgccagt gctgtagcct ttagccttta acaccccttt
     8161 taatgtatgg aaaacttcaa cacccatacg gatggcttcg gtaaacgaag gtgcaccatg
     8221 cggggcaatc ataaattcct gaaagtccac gttgttatcg gcatgtttcc cgccgttgat
     8281 aatgttcata cagggaacag gcaacaaata agcattgctc ccgcctaaat attggtaaag
     8341 tggcatcccg gcgctcatgg ctttagcacg ggcataggcc attgacactc caagaatggc
     8401 attggcccct aattttgatt tgttttgtga accgtccaga ttaatcataa aataatcaag
     8461 ttcacgctgg ctaacaaaac attttccttc gatttcttta gcaatgactt tgtttacgtt
     8521 ttcaacggct ttcaacacgc ctttacctcc gtagcgtttt ttgtcaccat cacgcagttc
     8581 gtaagcctca cgttctccgg tagatgcacc gcttgggacc attgcccggg cttttgtgcc
     8641 atcttctaac tctaaattta cctcaatggt aggattgccc ctcgaatcga gtatttcaat
     8701 tgcttcaact ttaatgattt tcatccgtaa ataatttttt aataaggtcg gatggaactc
     8761 gcatgatgaa ttttcatcct tgtttgtgtt taacgaatca tgctcgtttc atggcagtat
     8821 gtttttgaat taaaactaaa taacaactaa atgttgaatc caatcaataa acaatttatt
     8881 tcagctaagg ttaatcctaa tattgaattt tatttatcta aaccaacttc tcttgtatgc
     8941 ctaaggcttg tgctttagtt taatttcatt aaatgaaatg taagtatctg aaatgcagat
     9001 gaatatagct tgtgcgtgtg tcttcgcaga tttattagta gcaggtaaat tttatattcc
     9061 agcatcggga ttgtcaaata tcttgttgta caaatccttt ccaaagctgg tttgcagtgg
     9121 ttccccttcg accagcttaa aggtccgcgc cccgtcagct tgtctctcag ccctgaggaa
     9181 caaggcattt tctaactttc taacgtaaaa actttgggca aaatcgtaca aatgggtgac
     9241 aaaaaaaact tttaccccct tttctattaa ggcatttact atttgtctcc ctatttcgga
     9301 gccttctctt tcgttggtgg ctgcaaacga ctcgttaaag aggatcatgg aatgtgatga
     9361 taaattgtcg gcaatgctgc tcatcctgct tagttcttca tcaagttttc cgctgttcat
     9421 ggccgaatct tcttctcttt tgaaatgaga aaacaactgg ctacaggtat tagccgaaaa
     9481 agaagcggca ggtacaaaca tcccgcactg catcatcagt tgggcaaggc caatactccg
     9541 caaaaaagta gatttgcctc cctggtttgc cccggtaatt atcaccaggt ttttgttttc
     9601 ggctatagca tcattggcca ctaccttttg tttcatagcc aaagcgaggg atacatcata
     9661 caagccgcga aaaacgtgtg ttcgaaggtg cccaggcaag ggttggggaa acgagcaatc
     9721 agcccccagg ctggcaagct gtccgtgcaa attcaaacaa cctgtgtaga aggctatttc
     9781 agtctttaac atactgaaaa agctgaaaat gtgttccata gattgtgcaa gggcattggc
     9841 tacaacattg accccctcgt cttttatttc cctcaatgcc cttgccccgc tctcgtcgcg
     9901 tggatggaca ttgaaagtgt atcctggctt tttgcgcgga aaaagcattt gcacccatcc
     9961 ttgtttttgt tgagggagct tgcaaagctg gtaagggtag cctttgtttc cttttcccag
    10021 ttgggtgttg aacaaagccc catcgtgaaa ctcaagttct ttgatatgtt tccaggcttc
    10081 agccataaaa tcttcatcca actctttttg gagcattgca aaaaacctgg aaaagccctt
    10141 tgattgaaaa tgagaggcat gttcatctgc cagcgctttt aattgtttga gataacccag
    10201 caaatctttc aaggaagcaa tagagctata aagtaccgat gatggtgatg atgaaagaaa
    10261 ccaccaatgt tcgttccttg acaatagggc ttcggtggac aacctgtata tttccctaac
    10321 tacaagttgg ttttcaatgc tatctttcag gatgtcctgg cggtataata tggtttcaat
    10381 atcattgaat gtgcctgaca ggagagcttc tttagctatt ttgtagataa attcatcgcc
    10441 cgaagccatt gtggcaaata atgtccccaa ttctaagtct tgaaccaatg cagcgctgtt
    10501 cggcagaagt ttcttccggg tttcaaaatt atctttttcg aacatcagaa aaactttcat
    10561 ggccttaagg tataatgcgt tttttcaatt cactgtatgt aagctggtat ttttcagcta
    10621 ccgatattgc ataagccaag ccattggccg gcatccttga gaacttgaaa gtgcgttgtg
    10681 acgggtcgcc cgaaactact gcgccaacca tgctcaccgt tttttcattg aacgatgcca
    10741 gctcatctat aaatgttacc caaacacaaa gtacatcaag cctggtcaaa tcctccataa
    10801 tctttttgct cagaaaaata ccatcttgta atgtagtaga ggtcagtatt tcgttcatga
    10861 tgacaattgt atcgggggtg caatgcccta atatccaatg tagccttgcc aggtcgtctt
    10921 ccagcttgct tcgcaggttc agtacatttt cttccttttc gaaatgggta aaaatattgt
    10981 tgcaaagaaa aagctttgcc ctactgccgg gaacagggag cccgagccct gcaaggtagt
    11041 gcaactgccc aaatgtgcgg gcaaaagttg tttttccccc ttggtttggg cctgtaacga
    11101 ccaaaatacg ttcatggcct ttgaggtaaa aatcgttggc cactacggct ttcttttcac
    11161 ttacgagctt atgggccaaa ggcaggtcaa aacaagctgt aacatcaatt tccttgtcaa
    11221 cagccgaaac ctgcggatag caaaactcca ggccagcctt ttttatattt tcgatatact
    11281 cgaagtaagc caggtaaaac tgcacttcac ggtcgaacct gacaatggtt tcgtcctgaa
    11341 aacgggtatt ccctttgtaa aatgcatcaa gacgggcaaa agcatcgggg ttcaaatatg
    11401 caaccccatc caggattttg gcctccacgt ggttcatgcc caatggatat gagagcccgc
    11461 ttctgtaatc ttttacatcg ccttgcttga attttgcaaa agtagcttgt acttcttctg
    11521 tataatcgtc acccggctga aaatccaata ccctgacacg tagctccttg atatgtacac
    11581 tgtattttat tgaagataat tcatgtatta ttgccctggc ttctgcaaga aggtttttaa
    11641 attgcaaaga ttctgtgtaa cttgtgaggt agttacagaa ggtaatgaaa ccccgcgatt
    11701 ttatgggcaa atggggcaaa tttttagcca acgattccat ggcttggcca taaaagatga
    11761 caatatccag aaacacccgt tctttatggt attgatagta taatttttct gtttcggcaa
    11821 gctgtttgcg gatattgcgc atatctccct cgaaagcttt taaatacctg tataagtcct
    11881 ggcgttcaat atcttgcatt acctcctggc ggtattttat atcttctgtg tttgccaaca
    11941 aggtataaaa atagggctta aggttgtatt cttctttacc tttaagggct gtatctacca
    12001 cctggttgat gttcaaatca accataaatg ctggctcttc aagatgtaaa ctgtcatccg
    12061 ggacagtgct ggcaaacaat atgctttcaa aaaccatctt aatacctcca tcggggttta
    12121 aacaacattt ttcaacctaa gctcatagcc cttactttat ctccttaggg ttgcaataat
    12181 ccttttcgga tttaacaaca gttcgagggc atcgacaatg ctaaaacaaa caatatcggc
    12241 tgccataatg cttttggccg aagcaccttc tttttgtatt acggcaatac taagcgcgct
    12301 gcttttgagc atgagggcat catttgcccc gttgccaatg gcaactacat tttcagcgcc
    12361 taagctaatt gtatattttt ctttctgaat atcctgcata gatagcccca atatctcgat
    12421 agaacagttt aggcctgaca attggccctt agcagagcca aaagtgtcag ctgtcagtac
    12481 atagatgttt aacttactgc ctatttggcc gatgagttgc ttaactccat ctataagctt
    12541 gccgtctatg gccagtgtcc cgttataatc gagtaccaga tgtgccagct ctattttttt
    12601 atatccggga atatcaatcg ctatcatact ttaatcaatt aaggcagatg ttatttactc
    12661 aaagacagcc cgtaagcaat gctgttgaaa ttgtcgccca tgcggatttt atgaggccca
    12721 aacaactttt cgaagatatc tctgatgggc accacagatg cgctccctcc tgtaaggaat
    12781 actgtatcaa tatcggctgc cttgacattt gcgttctcca ttaatgataa aacgcagtgt
    12841 tcaattttat tggtttcgtt atttataaag ttacgaaact cttcaagggt cataagttct
    12901 ctgatgttga ttatgttgcg ggcaaactct accatggttt ctgtttttga cgaaagttca
    12961 attttggctt tttctatctg ttgaaaaagg gcatatacaa ggttgttgtc tataagcgcc
    13021 attaagtctt ccagcctttt gtcgcggcca gaaaatatgt aatagttgtt taagtcccta
    13081 cggagcttgg tttctttgag aagaaatgag cgttcccatt ttttgagctc gtggtgaatg
    13141 gtggtaggca cgtcaagctc tttgttgtat gaagcatatt tgacccctct gcccagcaac
    13201 ggggttagtt tatgccacat agtctgggag tcgaacatat cgccgccaat ataaagccct
    13261 ccgtttgcaa ttatatctcc cttgcggtct tcagcgttta tccggtcggg gccaagttcc
    13321 atgatggtaa agtcggaagt gccccctcca aagtctgcta ccaaaaccct ctcgtgctga
    13381 gtcaaggagc tttcataaga gaatgctgca gctataggct cgtaaagaag ctttacgtct
    13441 ttaaaacctg cattggaagc agctttaagc agtcttttga cagctatttc ttctttttcg
    13501 gggtcttcag aaaaaacaac cggacggcca agtaccactt gttcaatttc gtgacctata
    13561 gccttttcgg cttgctgttt gaaatgttta ataattaaag cagccagttc atcaggtgta
    13621 aaccttttgc caaaaataaa agtatgtata aagtttttct gttttaacaa tgtttttatc
    13681 gattttaaaa ggcggccgct catatctgaa ctaacatatt cattgatagc tgcttttcca
    13741 accgaatata cataatcgtt cgtgttttta aaatgcaaca ctgagctgtc attttctata
    13801 aaaatacttt tacgtgtatg aatatctgta atggcaatag ccgtgttaga ggtcccaaaa
    13861 tccagcccac actggtactt attcaaaatc atcaataata ctttttataa aaattatttt
    13921 ttccacaatt taaaagggga tttcaacagg ttggaattat aataccttgc atcatcagat
    13981 acttccttgc caacccattg gggcagttca aattgttcgc cttcagaatc caattctatt
    14041 tcggccacaa taagcccttc gttgtcccca agaaattcgt ccacctccca ggttttgctt
    14101 ttgtatgtga tggtatagcg ttttttttca attacgccat cggctaactt gttgataatt
    14161 tcggcagctt cattggcagg gattgggtat tcgtactctt ctctcgatat attgaccgtt
    14221 tttcctttaa tggtgataaa cccattatca cccattaccc ttacacggat tgtcttgtca
    14281 atactactat acaaatagcc ctgtattatc ctttcacctt tccctttagc aatattctgc
    14341 cagttttcta tatcgacaat aaattttctt tctatttcca taaagtcagt gtctattttc
    14401 gggctattag cacgttgcat ttggcatttt cgcttacttt gtctgtaaca tgccccaaat
    14461 cgttgcccca caagcctgag tggcctttac agcccatcac tatcaagtca acatggtttt
    14521 tttcggcata ctcaactatc aattttgccg ggttgccttg caaatgggca tattctattt
    14581 caatgccttc actttttgat atattgtgga ggtccttttt caacttggcc gaaaagctgt
    14641 caatggcttt cgattcttcg tcaatttcgg ccacagtttc gtgatagtag gccctgtacc
    14701 ccccaatcca taaagctgtc aactttgctt tcatggcctt ggcaaatgcc aatgaattgt
    14761 tcaatgctgt tttggcccct tcggaaccgt cgtaagctac taaaatgtgt ttgtacatgg
    14821 ttatttcctc ctttttgtta tgtttttctt actcattctt ctaagcctgc cccaggctat
    14881 catggtatgt atttcgggtt gaaagaattt ttgtgctatg agtgtaggta ttatggcgct
    14941 aagaattact actgtaacca ggatggtgta ttgtttttgg tcaataaggt ggttgtttag
    15001 cccaaacaat gccgatatgg tgccaaaggt aaggccggtg gccatcagca gtgtggtata
    15061 agaagattct tttttgcgca tctggaacga ccttgccaac ggatataccc caataaattt
    15121 ggtaactatt ttaataacca ggaacaatgt aataatgccc agcgaaaccc atattaatgg
    15181 cagcgaaacg tacaagccgg ctttcataaa gtaaaacggg gtaaaaatgg taaaggcaat
    15241 ggtacgcata cggtcaacca aggttttgtc gcgcacaaat accccggcca caactaagcc
    15301 tattaaataa gccggaagca cagcttcgct ctttgctgtg gtggccaaac ctcccaaaaa
    15361 gaacaacacg agcaggataa atttcacctc aggctggctt actttatttg tacccaaatg
    15421 tcctataagg tattttgtcc atcgtggcat aaaccaaagc ataacgcaca ttacaacaac
    15481 aaaagccacc atccaaaggt tgaaattggc aaacaaaacg cccagggcaa gcactgtccc
    15541 aaaatcggta ataaaacagg ctgcgagtat cattttgccc atggatgtat cgccaagctt
    15601 tttttcaatc ataactgcat atacaacggc aacagatgta gtagataatg ctatcccggc
    15661 aatctgggcc tggtgtatcc cccagccaaa aacatactgt gcaatgagcc aaacactggc
    15721 aaatggaaca acaaaagaca atatgccaat agcaagactg gcttttaagt ttgcttttag
    15781 tgaagccggg tcaatttcag cgcccgccaa aaaggttaga acaccactac ccagcatggc
    15841 caaaaaatct atccattggg tagtttggtg gatgcctaaa aaatttccgg ctatgacacc
    15901 taccaatatc tcgataaggg caacagaaat acctacacgg atagatatta agctggccac
    15961 taaagccatg cccatccaca ttgctgcaat taaccaaatg ttatccataa cacgtaattt
    16021 ttaccattta aaaaaacttt tcttttcgcc ttgtttgtaa aggactatgt ttgctttttc
    16081 catagttatc atacaaccat aacgcagctt ttcaaaccag gggagtattt tctcggcaaa
    16141 cttttcaatc ttttccgatt cgtcaactat ctcaattacc accgggagtt tttcggtaaa
    16201 ttcccaaaat ttaatagaac gcaccacgct gcttgacccg tagcccatca cccctttcag
    16261 cacggtagcc cctgccagtc catatcgttt ggctgcaaaa acaaccattt cgtacagtaa
    16321 gttatgtttg aatttgtcgg tattactgat aaaaatccgc aataatttgg cttctccttt
    16381 tatttccatg gttatattat tttagtgatt aggtttccca aataagtggc caaaatgcca
    16441 ataaaaacac tcaggcttgt atataaagca aaatagaaaa tattaccatc gcgaagcagc
    16501 gcaacattct cgttggcaaa agtcgaaaat gtagtaaacc cgccacaaaa gccaaccgtc
    16561 aggaacatgc gcaactctga agacatcgcg ttgccccgtt ctgaaatgcc ataaaacaac
    16621 ccaataagaa agcagccaag aatgttgaca acaaatgttc cgtaaggaaa agacgcaata
    16681 aaggtatttt gaataaaacg tgaagtaaga aaacgtgaga tgccccccag gaaactcccc
    16741 gaaccaataa acaaaagtat ttttatcata tatcctgcat actttaaggt taaacaataa
    16801 agtcatacag gagttatcag cttatttaag gcggttcgag gctaacccca tagccagaca
    16861 ccaaaggtag gaaaaaaatt aaaaacggaa agctattaat agctcatttg tagaataata
    16921 ccccgtatgc ctgaaaaact ctttaccttc tttatctaaa agcacttgtg aagatatggc
    16981 tgcaatgcca aagtatttca tcaaccgctg attttcgggc agcatgatgt ttataaagat
    17041 tacgtttacg cggtcgggga agcgggcctt tatttcggcc ataacctgtt ccatacgttt
    17101 gcaggccgaa cagccttttg ccccaaattc gagaaatgta aactggtagc ttagcccatt
    17161 tttagcgtag ttgtaggccg aatcgacaaa agccggtacc gagtgttgaa cttcggggcc
    17221 agcttgtgtt ttaatggctt ttgatgcgta attgtttaat gtatctttga atacgaaaag
    17281 caggacaaaa aacaataccg acagcaccat aaaagccgcc gacagcgcct gttttgtttt
    17341 agaagtcaac cgcagccttt tcataaacag cttcaattac ttcgctggta acaggggttt
    17401 ggcctttcac ataaccgagg tcggtcatac gcatgtattt gtaattggta atgcctgctt
    17461 tatcaagtat ctttttgcca caatcaaccg ggcagccatc tattagtaga aggtctgcct
    17521 ccttgaacat ctcgatggtg ggtttcacat cggctgctac agccgcaagg cagttcattt
    17581 tgcggatatt attgtccctc agttttcggg caaccaagtc cgatatttgg cccaaatcgc
    17641 atgcccccga acaggctatt acaacatatt cgccgctgcc acaaaggcag gtttttacgt
    17701 tattatttgt ttccatacat ttaaagttta aattctgtac taagcattat tatattggta
    17761 tgttttttat cggactgttt ggctttccac ccctgataat taattgaaag atttacagtt
    17821 ttaacaggtt ggtaatgaat ccccattata taggctttac cttcttttgc aatattccag
    17881 ctttgtgatt gaccagataa ggtattggat aacacattgt cgtagcgggc aaagatttca
    17941 attttaggat tcacaatgta agtgccaaac aaagaatatc cccaaaaatc atggtcattg
    18001 tagtttaggt tgttcaacct gtaattaacg tcaagtcctg cacggaaaac tttatccaac
    18061 tggtaacctg tgaaaaacga aaagaggtgt tgcgttgcat tatcgctcgg gtcgcccgat
    18121 tgtgtattgt cataatacag gcgggttaac aatcctttta aaggtttaat gtcaatacct
    18181 gttgcatatt ttactttacc gtaatcatct tgtttaaacc ttataccttc tccgttggta
    18241 acagcaacgt caaaagccaa aatgttattt accttaacaa acccgattgc cccgaaatcg
    18301 ccacttgcag ttccataata tctgtcttgg aaagtttcat aaacatagcg atagccccaa
    18361 aacttttcct gagtgatata atgattttgc aaaataccac ccacttgcaa agtaaacatt
    18421 tcagaaggtt tccattgaat aaaagcaaac ttcaggaatg ctgtataata gcttccttcc
    18481 ttggaagtgt tattgactgt ataattttta cctgtagaat cggttacaac aatattacca
    18541 acagtagttg gcctaccagc agcatcaatt atcaatgtac ctgaaaaact atttgaaaaa
    18601 ttataattat acccaaagtg ggcacgggta ataccaaagc ttacaggttg tttggcatct
    18661 ttagttgcgt caagcttgga atacccaaac acttgcacaa taggtttgcc cgaaggttta
    18721 aaagttgtat cggtatcttg cccgaatatt tgtacactat aacatataca tgtgaatagc
    18781 aacaaaaacc taagttttgt tttcatttac ggtttaattc tattgtatat acctgttaaa
    18841 tattggtcgt aatagccttt aatggtttct tccacttctg ccattggttt taccggaaca
    18901 aggtcggcta cttcggcata aggtaaacct gagttgaaaa gggtttcggt tgtaccccaa
    18961 agcatatttg agatagtttt agcggcctct tgttcatcca tgccaaacga aacggccagt
    19021 tctttaagct tttgcaactg gaagttaaag taagtatgcc ccatggcgct aataacagca
    19081 taagcttcaa ttttgttgtc ggctacttcg ggctcatagc ctaatggccg aaacagttcg
    19141 agaaatgctt tctttttatc agcatcaaaa gaagcagaaa acgaaaccgg gttaacgcct
    19201 ttgtttacaa tggttgaagc gctggggttc atgcgggcaa tgttggcaaa accacccagc
    19261 aaagcagtca gtttttcaat agtaaacttt ggcgctaacg aaagcaatac cgaaccgggt
    19321 tgcaggctgc ctttgatttt ctcaatggtc tccattacca ttggggggtg aacggaaaga
    19381 ataacaagtt cggctgttgc agcttcatca acactggttg tagtggtaac ctgtggaaat
    19441 ttagctttta cagcttttaa tgcttcttcg cttacatcat acacaactac tttgtcaaaa
    19501 cgtacattgg cattgttaaa tgcctgggct aaaatgcggg taatacggcc gccgccaata
    19561 aaactgattg ttttcatttt ttttaatatt aagggttaaa aattattcaa catattttgc
    19621 aatggttttc tttattatgg gcataatcac catcaccacc ggaagggctg ccacgaaggc
    19681 aagtccccat gatttcatcc atttggcaat aaatccggca tggtagccaa agtttaccga
    19741 tacacttaca aacgttacta caaatgtcat taccaaaacc atgatgatta atgtaacttt
    19801 catttcattc ttttttgcga ttttcatttt ttttattgtt ttaaattgca ccaaatatta
    19861 gtccggaaat tgtggccatt acaattacca atgtaacata aactaccgtt ttttgggtac
    19921 ccattacgcc tcgtattacc aacatgttgg gtaatgataa tgatggcccg gccaataata
    19981 acgctaaagc agggcctttg cccatacctg aagcgattaa tccttgtaaa ataggtactt
    20041 cggtaagagt ggcaaagtac ataaaagcgc ctacaactga agcaaaaaaa ttggagaata
    20101 ccgagtttcc gccaactaaa gcagaaatcc attcgttagg gattatgcct gcaattgttt
    20161 ttccatcatg ggtggagcct aacaaaaaac ctgctgtaac aacacctatg gcaagcaagg
    20221 gcaggatttg tttggcaaac ccccaggcag aaattgtcca ctctttattg tcttcgtctt
    20281 ttttgtctaa aagtgttatg gtacttaaag ctgcaatgcc aactatcatg ggtacaagcg
    20341 gggcaagttt gacgttttca ataaacgtcg atgataagaa agctgatgta acagttgcca
    20401 cggttgccag taaaacccat tgccatttta ttttcaagat atagattaat gaataaccaa
    20461 gcagcaatgc aaaaaagccg gtgatatacc atttataagc ccatatataa taccaggcac
    20521 ttgaagtatc gctacttgca ggactgcccc agttagcaaa aacaaggatt aatactaatg
    20581 taaaaaaatg gaacgaagtt tgccacatcg gacgtttttc agcctgaaat tcaatattca
    20641 tctgttcttc acgctttgct ttttcttctt tacggtatat gagcgacata gccactccta
    20701 taatgatact aaaagatact gctccaatag tccgtgcaac acccatctca aagcctaata
    20761 tacgtgcggt taagataatg gcaaggatgt ttattgccgg gcccgaatac aaaaaggcaa
    20821 tagccgggcc caaaccggca ccacgtttgt gaatacttga aaacaagggc aaaatagtac
    20881 aactgcaaac ggcaagtata gtgcctgata cagcggcaac tgtatatgcc agccatttct
    20941 tggcattagc cccaaaataa cgtaatacag agccctggct gacaaatatt gatattacac
    21001 ctgcaataaa gaatgctggt aacaaacata aaattacatg ctcacgggca taccatttta
    21061 caaggtcgaa cgtggctgca atggccgtat taaacctgac actttcgatg ggcagaaaaa
    21121 atgctgcccc aaatattact gctatccata gcaggatttt aaactctttt tttgtttcca
    21181 ttacttcttt tgttttattt agaatttcgt ctaaacccga aatatgtttt caaatttttt
    21241 tataaataaa cccttatgat atagtagatg ccaaccagaa tgaacaatat tgctataatg
    21301 cgacggaacc acttttcaaa ggtcttcacc ttatggtaaa catgcccaat gcccgatacg
    21361 gtgtatgcaa gcacccatgc aaaaagaatg accgggatac ccgtagccag agcaaaaatt
    21421 actggaaggt acaaacccga tgcgctggca accgtcatgg gtacaagcat gccgaaatat
    21481 aaaaccccgc tgtaagggca aaaagctaac gcaaaaacaa tccccagcag aatggtgtct
    21541 atataattcc atgtagcttt gttttccatt cgggaagtta atttgcccat gcctggaaat
    21601 ttcattttga tgacatccag cataaacaac ccaataatga taagcaatgg gccgaggaac
    21661 ttttcgccat actgctgaaa aataccggaa aacttaaact ggtcaacccc aagaaaaatc
    21721 accagggcta ttgcggtata tgaaatggcc ctgccaaggg tgtaaagcaa accattgata
    21781 aatacacggt ggcggttttc aatatctttg ctgataaagc ctattgctgt aatgttggtg
    21841 gccagggggc atggactgat agctgtcatc aaacccagta ccaatgccga aagccacggc
    21901 attgtactgt tttctaatat gctgttaaaa aaatccatgt tgtgctactt caaaagctca
    21961 tcaacttttt ctttaataac agatcttaat ttttcagggt ttgagcgggc atacataaaa
    22021 ccttcgttgg taatgttgat ttttgtatcg ccttttacta tcaataaagt ttgtcctgat
    22081 acttttaact tttcagcaag ggctttgctt gaagcttcgt ctaaattgac cgactgaaaa
    22141 gaaactttgt cgccataaag ggctgcgatg ctttctttgg cttctgcttc aaccgtctta
    22201 caagtaacac agcgactggt aaaatgaaaa taataagctt ctactttagc cgaagcattg
    22261 ggtttatcct ctttttttgt agtttgtgca ttgcacgata aactgcccaa tgtcattaca
    22321 agggcaaaac tgaacattac aatctgtttc ataattattt taatttgaag attatctaat
    22381 ttgaaaatta tacaatttga gaatttgaga atgatttgct tattaaccat cttcaaattt
    22441 tcaaaccaat acattttcaa attgctttta acaatacttg ttttatttcg tcggcagaag
    22501 gtacacggcc ttttatttca accttttcgt tcactacaag ggcaggggtt gccataatgt
    22561 tgtatttcat tatctccaca atgtcctcta ccttggtaat attggcattt atgccatttt
    22621 gttcgaccac ttcacgggtt atcttttcta gtgttttaca tttggcgcaa ccagagccta
    22681 aaatttttat ttccatacct taaatttatt tgataattat acaatttgat aatttgaaga
    22741 ttgaagaatt gatttttatt ttcaagtcaa ttcatcttca aattattata ttttcaaatt
    22801 aaaaaattgt atcattgttt tttttgcttc ttcccaattt tcgcggttta tacaatactt
    22861 gatatacgga gggtttattt caccctgtat caatcctgct ttttttagtt cggccaaatg
    22921 ctctgataag gtagagcggg caatgggcaa ttcttcggcc atgtcgccac tgtaacagca
    22981 tttatctata ttgttggcca ggtaatcgag gataaaaacc cttaccgggt gcgaaagcgc
    23041 tttggcaaac cgggcaacct tctcttgtga atcggtgtaa tatttttctt tttctgacat
    23101 tgttagaaat atgtttcgca aatatacgaa atatatttcg gaaatttgcg aaatggatta
    23161 aaaattttta ttattttgat gaattatatt gttttaaacc ccagtgcatc catcaacttt
    23221 tcgttcagct tgcggttctt acctttataa ggatggcaat ggatgtggat ggcggggttg
    23281 aagtttagct ctaagatggc gtaattgtct ggcctggctt cctgatagta atcttttatc
    23341 atcatatcca gcccggtaat cttgacattg agtgcagatg cagcttttat ggctatttgc
    23401 ttgtacgaat ccgggatttc gtctgtaaaa tcaatgctat cgccgccagt gctaatgttt
    23461 gagttctcgc gtaggtaaac aacttctccg tttgccggaa cgtcatcaaa agtcttatgt
    23521 tgcgacttca gaaacatggc ttcggcctcg cccagttgga ttttttcaag cggggtgcgg
    23581 tatcctttac cacgcaaaag gtcttggttt tttatttcta ccagttcgcg aatggtcttg
    23641 tttccatcgc caaccacatt ggccgggaca cggtgcagaa tgcctgccac ctcgttgttg
    23701 atgacaaata tcctgtattc cttgccttca ataaattctt cgataagaat ggaggtgtcg
    23761 tgctcaaaag caatgtccac ggccctgaaa aaggttgttt catcggtgtt ttctttgaga
    23821 atagtgatgc ctatgccgaa attggtctgg ttgggcttaa taacaacagc tttgcccttg
    23881 aataaggcga aatctgtttt tgcagcttca gcattggtat attcatagcc ctcagggacg
    23941 cggatgccgg cttccctgac aatctttttg gtcacttgct tgttttccat ggccaggatg
    24001 ctggcgtagt tgtctaacga agttttggtg gcttgtttta cgtattggat attcccgttt
    24061 tgttttaacc tcacgaaatt ctcttttctg tctaaaatat caaataaaat gccccgtttt
    24121 acagcttcac gcaaaagcaa ctgtgttgaa agctccatgt cttccagccc gataaagttg
    24181 tagctcttct gttggctggc atttaaatag cttttagctt tttctaaatg aaaaggtaca
    24241 tatccttttt gcaatatacc atcgaggact tcataaactt ttcttttctt ggggttttta
    24301 attagtttgt ttgctacatc aattgatttt attatacttt cattatttac catcaggtct
    24361 tgaagtaaat tagccatgtg ctgaatatgc tttttggctt ttgcccatac atctgcttct
    24421 tcctgatttg catgcgggtt tgggattaag ccgtaaagcg atacatagcc atgatattgg
    24481 ttggcatgcg actgtgctgt tgcatcaaac gaatcgggtt cttctgaaag cagcccataa
    24541 atggccatag catgaaggaa gtgtaaagct tcttcgctta taccggcttt tgtcaacggg
    24601 tcaatgtcga taaaccgcaa ttctacataa gttatatgct tagggtcttt gataaacttt
    24661 ggccgaatgg acgaataaag ctctttggtg gaggctaact ttccatcttt gaccatttgc
    24721 tcaatgcttt gttggaaatt ctgtaccgag ctgtagtcgg ggtatagctc gccaatgttt
    24781 tggtaaccat agcaactatt gcggatagaa aggcctatta cgtgtttgtc aatagtaaaa
    24841 ggcgattgct tgcatttaag ttccatcgaa gggtcaacta ccgggctgtt gccataaagc
    24901 aacacataca gccatcgcag tctgagcatt tgtcgggtaa ctttcaaata cgcttcatct
    24961 ttgaactctt caaaagtcat aactgagttg ctcttgtgat atagcatttc catcagcttt
    25021 tcgccaaatg acaggttgaa atggatgcct gaaaatagct ggtttttggg gccgtatttg
    25081 ttggccaaaa attcgcggta ttgttcggct tcttttcctt catcgtcaaa acgggctata
    25141 ggaataaggg tttcgtctgg taaaactggc gggatgctct gaggccaaag gtattcatct
    25201 tccagctcaa ggctgactat gtcgtgaatg gtttccagga acccaaccgt ctctttgata
    25261 gttggcaggg gcggggtaat catttctacc tggctttcgg aaaaatctgt agtgacgtat
    25321 gcatgtttac gcttgttgcc aaatgttttg gggtgaggtg taagcgaaag gttgccgttt
    25381 ttgtccacac ggatgttttc tttttcaagg ccaaacaagc cttctgtcca gatattacct
    25441 ttgatctggc ttactaacgg ggtgaaaata tcgttgttca tagcggttga ttttttaatt
    25501 gaagcttgaa attttgatga gttggaggtt ttgcagcatt tgtctattaa agctggtttt
    25561 tccttcaatt ttttatttat taaacatgct gtactcatga ttttacggat tgggtgtttt
    25621 taataaatat cttaaaaaga ataacataca acagtacaag cagtgagcct ttggcaatgc
    25681 tgctgatgct gatactccac catactgagg ataaggagtt ttcccagaaa tggataaaag
    25741 caatggccat aggaattctc aacacggtaa gtgtaatccc tgtaatggca ggtatattgg
    25801 tgcgccccca accaaagaac gccccggtag ccatcagttc catgcacata aatagctgcg
    25861 aaacacctaa aataagcagg tattcgcgcc ccatggcaac actttccttt tcatgaagga
    25921 aaatagaaaa caaggagctt ggaaatgcaa tcaaaatggc agttgtaata ataccaatga
    25981 tgaaagcaag caccatccct gaccgaaacg tttcccattg ctgctggtaa tttttggcac
    26041 cgtatgcttt accggacaac gaggataagg ccgacatgaa cccgataatg gtcatgtacg
    26101 aaatggcttc aatctgaatc cctacttttt gaacagcaat ggcgtttgct ccccagttgc
    26161 tgatgatacg ggccatggca atagcaacaa tggtaaagga tacacgctgt atggtagggc
    26221 tgatgcctaa cccgatgatg tttttcagga ggactttacg gaatgttacg cctttaggcc
    26281 ttagcatttc atttttatac aatttattgt aaaaaactat tgtagcaaca gcctgcgaaa
    26341 ggatagtggc cacagcagcg ccataaatgc cccatcctgc aaggaaaata agcaaagggt
    26401 ctaaggctat attgatgcca aaagccaggg cgtttatcct aaaaggggtt ttactatcgc
    26461 ctgagccgat aaaaacattg gtaaaaaaca ggttttgaaa ggtgaacgga atgctcaagc
    26521 ctgtcaacat caaataattg gtggtggcct gttctatctc agggtcattc aggcggataa
    26581 tgccaaccag gaagcttttt gttgccagca ataccagata gtaaaggact gctacaatca
    26641 atgttgctac aatgccactt tttacatagc ttttggccaa aagattgttt ttctgcccga
    26701 tgctgtgcga tacttttatt cctgccccta cagtcagcaa tgaagataag ccccagccaa
    26761 ggttcatata aaatcctgct gaaccaactg ctgcaacagc gctacttcca agctgcccta
    26821 caaaaatgat attgacgaaa tggtaggcca tagacatgaa cgacgtggca ataactggca
    26881 atgccaggca tatcagctgt ttcattgttg aacttgcttg acatttattc agaatgcccg
    26941 aaaaatggag gtatttcatg ctttgcagtc tttcccctcc tttataagga gagcttgtcc
    27001 cttctcaggc gggaggttgg ggtggttaag aatcttaatt ttccttgaat aaacctgtac
    27061 ttaaatactt ttcaccgcta tccgggaaga tgacgacaat attgcccgaa tcaattttcc
    27121 tggctacttc cagcgcggca tacatggctg ctccactact catgcctaca aaaatccctt
    27181 cttttctgat aatttgacgt gccatatcat aagctgcttc tgtttctacc ataatggttt
    27241 cgtcaatctg cgaaggatcg tatatagcag gtacaatggc ttcttccata tttttcaaac
    27301 cttgtatata atggcctttt acggggtgcg cacatacaat cttaatattg gggttgtttt
    27361 ctttcaaagc aaggcctaca cccatgatag tacctgaagt tccaagggca gaaacaaaat
    27421 gcgtgatttt gccattggtt tgtttccata tttcttcgcc ggtagtcttg tagtgggcta
    27481 ttttgttgta gctgtttgaa aactggtcgg gcataaagta tttctcagga tgggccttga
    27541 ccagctcttt ggctttaatg atggcaccgt ctgtaccgag tttctcatct gtcagggtta
    27601 cctttgcccc gaaagcctta atcattttga ccctctcaat ggatacacct ttgcttatca
    27661 ctatttccac gtcatagcct ttcattcggc ctatcatggc caatcctatg cctgtattgc
    27721 ccgaagtagg ttcaatgatg gtttttccct ttaccaacga accttctgct tcggcttgtt
    27781 caatcatttt gagggctatt cggtctttga tgcttccaga cgggttaaaa ccttctattt
    27841 tggcgctgat cgaaatattt tcgtatgggt ttagtttgtt aatccttaca agtggcgtat
    27901 tgcctattgt ttctaatatg ttttcgtaaa tcatgttttt aattttaatc attaataggg
    27961 caacagcctg ttgacctgag tttaaccatc tgactggtaa tcttttcaaa taacctgatt
    28021 agttcatcct tttcctggtc ggataatacc gacagcgttt tttcaatata cttgtcattg
    28081 ttgccattga tggtatggca aacgtcaatg ccggcttgtg taagctgcaa aattgaagta
    28141 cgtctgtctt ctgcgggaat agtgcgctgt ataagcccta tgttgaccag cccgtcaact
    28201 gtacggctaa tagtactttt gtcaagtcca agattattgg ctaattcagt caatgtttct
    28261 ttttctttgc tttctatttc aagcaaagta tggcattgag ccagggtcac attgctacaa
    28321 caacattcat tcgtctgcac aaaaatctcc cgttcaaacc ttctgagcag ttccctgaat
    28381 tttttaatgg tttgattttc tgtatttttc attttacaaa gttgtaattt aaaactgttg
    28441 caagttacaa ctataatttt tgttgaaaag cttttttatt tttttaacaa aaaaatacac
    28501 taactttact tgttattaaa agagaataat tcttatctaa tagttagtat tatgaaatta
    28561 aatagtatta gtagtgcgtt gaatgaaaaa gggttaaaag ttactccgca aagaattgcc
    28621 atacttgagg ctgtttttaa actgtgtaat catcctacgg cagaacaggt gctggattac
    28681 atcaggaaaa agaatcccaa tatctcgctg gccactgtgt ataaggtgtt ggatgctttt
    28741 gttgaaaatg gtttggtaaa gactgtaaaa actgataagg acattaagcg ttatgatgcc
    28801 tgtgttgaaa agcaccatca tatctattat tctgaatcag atatcattga agattacatg
    28861 gatgctgagc taaatgattt gcttgaaaaa tattttgaga ggaaacaaat aactggtttt
    28921 aagattgaag aaataaaatt gcaaatagtt ggaaaatcaa catctaaata ttgaaaatga
    28981 acgtgtttcg atttgtacta atagtgttag ggacattgtc gctggcgttg gggataatag
    29041 ggatttttgt gccggggtta cctacaactc cttttatttt gcttactgcc gggttataca
    29101 tgcgaagctc ggactggctg tacaagtggg tgataaacaa caggtttgta gggagttatg
    29161 ttcaaaattt tcaaaaaagc aatagtattc cgcttcagac caagttgtat tcaatagcaa
    29221 tgatgtggac aatgataggc atttcaacgt ttttttttat agaaagccta tggggaaaag
    29281 gattagtggt attggcaggt gtggttggaa ctgtagtagt gggtattgtt attaaaacga
    29341 ggaaacatag taatttgtag ggttttaata tttgttataa tatttctgac acagttatat
    29401 gcaacttatt gtaaatgatt tatttgattc ttggttcttg gttcttacgt tttatatatt
    29461 tagatgttta accaaaaaat aattgagatt atgaaaatta gtaaaaaaat agaagaagtg
    29521 ctgaataagc aggtaaatgc cgagctttgg tcagcttata tgtacttgtc gatgtcggct
    29581 tgggctgaga ataaggggtt taaaggtttt gccaactgga tgcgtgtcca gtttcacgaa
    29641 gaaacctctc atgccctgaa aatactggat ttcattctta cccgctcggg cgaggttaag
    29701 cttgagccta ttgcagctgt taaaacctca tgggacagcc ttctgagtat gatggaagaa
    29761 acatatgagc acgagtgcaa ggttacaagc cttattaacc aatgttatga agtggctttg
    29821 gccgaaaaag actatgctac agcaacaatg ctgcaatggt tcattgaaga acaaattgaa
    29881 gaggaagcca atgccctcga aattattgat gtgctgaaaa tcactggtga aaagagcggt
    29941 ggcgtttttt acctcgacaa aaaattgggg caccgtacat tttctgatga taccaagaaa
    30001 gaataacttt ttctttcaat aacttttttg aaaatcttga ggttgggttc tttatttcaa
    30061 aatagagccc aacctctttt tctttatact tccgctcgtt tttttggaaa cggccttttt
    30121 taactttttc tgctagctca aaaatttctt tgatgctctt agtgtgagaa aaacgagcta
    30181 catggtaggg cgggctttag tattttattt ctaaccaatt ttcaaaatta ttgatattaa
    30241 ctacttcaaa tgaagaatct ggatatgcgt ttttccattg tgtcgggatt ttaacctttg
    30301 attccttcca ttttaattca tatccaaata aagttccatc acgttcctct acccagtcta
    30361 tttcttgttg ttcataagtt ctccaaaagt aattgtttga agagattctt ttatattctt
    30421 gatatttaat ccgttcacta atcatatagt tttcccataa ttgaccaata tcgttacgag
    30481 actctattgg gttgaaatta gcaattaccg cattccttat cccattatca agaaaatacc
    30541 atcttgaact tttagtaatt tcttttctaa ggtttctgct aaatccttct actttatgaa
    30601 gaatataaac cttgctcaat aagtcaagat atttctcaac tgtgtttttg ctaattgata
    30661 attgttggcc taactcttga agagacacct ccttgcctaa ttgaaatgca atcaaacgca
    30721 ataaattgaa tattgtttga gagtttttta tgttttcata aaccagaata tcctttaaca
    30781 gataggaact taccatttca tttaaatatt caatcttatc ttttttgtca ggcaaatgaa
    30841 ggagttctgg atagtttcct aaaactaatc tttcccttaa tttatccgtt tttgatatac
    30901 gatcttcgat ttggctatat tctctttcgg acaaagcata taaattaaat gtatatttac
    30961 gaccagttaa tggttctcca gcctctcgat taatatcaaa tgatgatgag ccagatatta
    31021 ttattttaag tccttcaatt tcatcaatca ttaatttaag tttcacacca atatcaggaa
    31081 ttttttgagc ttcatcaata aaaagaaatt tatacgaccc taaaagttgc ctataatttt
    31141 ctatcgtcct tattgctaat ttatcatgga cattaatatc ttccccattt aatcttagga
    31201 ctggttcatt aaccttttcc aaaatctttt ttacgagcat ggttttccct acacgtcttg
    31261 ctccatatac tattacaacc ttatttgatt gaagcttatt taataaattt tcagttatct
    31321 ctctgactaa ataattcata attccgtcaa ttttgtgacg caatttagtt tatttccgtc
    31381 aaatatccaa attatgcaaa aaaatattag aatcaaaaaa acagcgatgc tgcaaatact
    31441 gccactcctg taaagcaaaa tgcaattcac ctttttatac ctcggtaata ctcgctttga
    31501 taaccaacgg atgaaataag ctgataataa gtccatcgag atttgtattt tttttgaata
    31561 agtgttctaa ctcacttttg agtttatggt ttcaactttt tcctgaaaac tttgtttcaa
    31621 ggctaaagca taaataatgt cttcttcaaa tttgtatatc actcaggata ctgagctgtc
    31681 tgatattgtc agggcttatc ccgggtcaat gctatttctt gagcattttg acattcatgt
    31741 accttttcaa acaaagacgg ttgacaaaat atgtagtgaa cacaatataa gcagcgattt
    31801 gtttatcgct tttgctaatc tttatctggg aaaggattct taccaaattc ccaagctcag
    31861 attttctgac cttccggcta tcatcaggta cttacataac aaccataaat actatttgga
    31921 cgagatatat cccaaaatac agcagtgtat aaagttaatt atgggattga acgacctgga
    31981 tgaaagcaaa ctggtggaaa aatttttcca ggattatttt aacgaagtga gagagcacct
    32041 taactatgaa aatagtgttg tatttccttt tgttactggt ctttatgatg tttttataaa
    32101 taaaatccct tacaggcaac gcaacgctta ttcagtaagc gaatataaag atcatcacag
    32161 cgatattgaa gagaagctcg acgacctgat gagcctgctg ataaagtacc tgcctcacaa
    32221 aaatgaccaa caggcaaggc gcgaattatt tcacctgttg gttgagttag attacgattt
    32281 gaaggtacat gcccgcatcg aggatttgat attggttcct ttggttgaaa aactggaaaa
    32341 acaactgaat gcaaagccat gagcaaggta accaatataa tagttgtcga aacatcatcg
    32401 gtgctttatg aagggcttgt tcaggttatt tcgcatgctg gcatgccttt gcatatcagg
    32461 caggccggaa atattgatga tgctgaaaaa tttatttgtg ctaaacctga aagccttgta
    32521 attgttaatc cggcaataat tcagcataat gtaaaaagct tccaatcgct taagaataat
    32581 tgggacaaag cgcactggat ggcattggtt tacttgcatt atgaccagca ggtcctgggg
    32641 ctttttgatg gtattattag tatttcggat agtccaggaa caattatttc tactattaaa
    32701 aaagttcttt cttctaaaca ttcccaaaat acttatattt ctgaagatat tcttagtgaa
    32761 cgggaagtgg aagtcctgca attattggct gttgggcttt caaacaaaga gattgccgat
    32821 aaactgagca taagcattaa tactgttatc acccatagga aaaacatctc gcaaaagacc
    32881 ggaattaaaa ctgtctcggg tttgactatc tatgctgttg taaataagta tattacgctt
    32941 gaaagtgtaa aacctatggt ttaatatatt attctaaaga taatttatta attaacgtga
    33001 gttcgatgta aaaaatcatt ttcattgagt tattcagtaa taagccatta tttccatgca
    33061 acttttttat cgaaaaaagt tgctcaaaaa ctcttatcgc cttaactctt tcaccctcga
    33121 agccttttaa tgttgcaata tgcaaacaaa ggaatgttag gcttctcggc cgaaatcgaa
    33181 caaaaaggcg atgaaaagga atttttttct ttttaatgct tcgccttttt catagaaacg
    33241 tctctgctat tggcctgacg aaaatggcgt taagaaaaaa gctttatcga cgaagcgagt
    33301 tattgcccgg agccgttgat aaagcttttt ttagccaatt tacgtcaagc cttgaatttt
    33361 tggaactttt gcttcaaggc aaaagttagg aagcttttgc taatacctgt aaatattagc
    33421 tatgtaagca ttcttagact ctcgttatta taatgttgaa ttgacgttaa tttagcctcc
    33481 tcatttatga tgaggattat cttttttttc atcatatctt ggtatttcac acctcttggg
    33541 ccaaagctac ttttgctgtg taaataatta aaacacaaca aaaaatgaaa aagatataca
    33601 cgttggctct ttcgcttttc ttcgttacat ccatcattgc ccaaacgtca gaaaacaatt
    33661 cggacgaccc tttgctgctt gaggcgtctt atacctgcga taacgtaagc aacctggctg
    33721 ggggtatcaa aactggctat ggttatttgg gcatggctac tatacgtatt ggttttgata
    33781 ccgaaaaagc cgggctgtgg aagggcggac agctttttgt caatgctgca aacactcacg
    33841 gggcaacacc ttcggctgat ttgctcggcg atatgcaggt agcttctaac attgaggcag
    33901 gcaaccatac atacttgcag gaattttggc ttaaacaggc tgttggccat gtcgaactca
    33961 ctgttggttt gcaggatttg aatgtagagt ttgccaattc tgagcatggc gcgttgtttt
    34021 tgaacagttc gtttgggatt cttcccatta tttctggcaa cgttgcagcg cctatttttc
    34081 cgcttacctc gttgggcttg acagccaagt ggaatataac cgaaaaaacc tcatggctca
    34141 acgccgctta tgatggcagt cctacggatt ttgactataa cccgcacaac attaagtggg
    34201 agtttgtttc aggcgatggg ttgctggcta tttctgagtt acagcaaagc cttgatattg
    34261 ttggtttgcc cggaacgtat aagttaggaa tttattcaca tagccatatt gtcgaaaata
    34321 actttaattc aacattagcc gactcgttaa actacaacac ttttggggct tatgtttatg
    34381 cagaccaaaa agtatgggcg caggccgaac gaagccttgg attttttgtt caggcaggtt
    34441 atagcccctc taaaatcagc gtcaataatg cgtatattgg cataggggcc aacctgaccg
    34501 ggatgttgag caaagctaaa gatgatgtgc ttggtctggc tgttgcccac gaaaacttta
    34561 acaatggctt aggtagcgaa accacgatag aactgacctg gcaaaaacag ttgttcagaa
    34621 acatttttat acagcctgat ttacaatata tcattaagcc ttccggtaaa aatagtggct
    34681 tagaaaattg tttggctgga attatcagac ttggtttggc tttttagtta atcgatattt
    34741 ttaataactc gaaataattc ttgattaata atgactcatt gaaattaggt ttatgaatgt
    34801 tattatagca atgactttga aataatggct gcgaagcaga caaatagata ttccttattt
    34861 tggctgtgaa ctgtctgaac gaatccgata ggcggagtga gttttcacag ccagtctttt
    34921 ttggtttctt tttttgacaa aaaaaagaaa caattgaata acagaatatt agtctattac
    34981 tgcagttcta tagttgaact ctcgttaaac aatatataat gcaattatca gaattaacaa
    35041 ataatcagca ggccgtaatt gccaaagtat ctggtcacgg cgcttttcgc aaacgtatta
    35101 ccgaaatggg ctttgtccgt ggcaaaatgg tgaaagtaat caaaaatgcc ccgctacaag
    35161 accctattga atatgaaatt atgggttata acgtgtcgtt gcgtcgtagc gaagctgcat
    35221 taattgaggt tgtccgtgaa gaaggagcgg aaacaatcgt ccagtcaggt tttgaaggaa
    35281 caatagttga cgatcgcctc aaaatttcgc taaaagaaaa gggtaatact atccatgtgg
    35341 cattggtagg gaatcccaat agtggcaaaa ccactttgtt caaccatgcc tcgggttcgc
    35401 acgaaagagt gggtaactat ggcggtgtta cggtagatgc caaagaagct tatattgctt
    35461 ttgaagacta tcagttgaaa gtgaccgacc tgcccggcac gtattccatc accgaatata
    35521 cgcccgaaga gctttttgtg cgtaaccata ttgctgagaa gatgcccgat gttgtggtca
    35581 atgtgattga tgcttccaac cttgagcgta atttattcct caccacacag cttatcgaca
    35641 tgaatatcaa agtggttatc gccctcaata tgtatgatga gctggaacaa aaaggcattt
    35701 cgcttgatga tgatgccctg ggtaggatgc ttggaatacc cataattcct actgttgctg
    35761 tgaaagggca gggaataatc gcgctgattg caaaaatagt ggaagtgtat gaagacagag
    35821 acccggtggt acggcatatt catataaact atggcaatac tattgaagat tcaattgccc
    35881 gtatccaggc tgagttgaag aaaagtaaag agttgaccga ccgcttttcg acccgctatt
    35941 tggctattaa gcttattgaa aacgacaaag ccacgctcag tcagcttaaa agctttttta
    36001 attttgaaaa tattgaaaaa ataagccagg ctgagataca gaagctcgaa aaagagtacc
    36061 acgagaaatc agaaacggtg attgccgatg ctaaatatgg ctttattgct ggggctttgc
    36121 gcgaaacttg ttcaggttgt gctgaaaata caaccaacaa gaaatggaat gttgattttc
    36181 tgctaaccca caaatactgg ggattcccta ttttcttctt tttcatgtgg ctaatgtttc
    36241 aggctacttt ttcactgggc agcatcccaa tggattggat tgacgcaggc atagccttta
    36301 ttggaagcca gttgggtaat ctgcttcccg aaggagccgt gcacgacctc cttattgatg
    36361 gcatcattgg cggggtagga ggggttatta tatttcttcc caatatcctc atcttgtttt
    36421 tcttcatctc actgatggaa gatacaggct atatggcccg tgtttccttt atcatggaca
    36481 aacttatgca caagattggc ctgcatggca agtctttcat tcctttgctg atggggtttg
    36541 ggtgcaatgt tccagctgta atggctaccc gtacgctcga aaatcgcaaa gaccgcataa
    36601 tgactatgct catcaccccg tttatgtcgt gtagtgcacg tttgccggtt tatgtgctga
    36661 tcatttctgc tttcttccct aaaaaccagg gattggtgtt gttttcggtt tattttatcg
    36721 gaattatcct ggctgtgctg attgcgctat tgttgaagaa gacattattt gcaaagcagg
    36781 acgtcccttt tgtaatggaa ctaccaccat acagggtccc tacattgaag aacacttcaa
    36841 aacacatgtg gttcaagggc tcacaataca tacataaaat gggtacggtt attctcatgg
    36901 cttccattct tatctgggca ttgagttatt atccccgaaa tgttcagtat gctgtgaatt
    36961 atgatgaaca gattgaacaa attacagcaa atgccacttt ggctgattca gtgaaagaat
    37021 cgaacatagc tcaggttgaa ctaatgaaag ttggagagca tcaggaacag tcatatattg
    37081 gtcgtttagg aaggtttatt gaacctgcaa ttcagccgtt gggtttcgat tggaaaattg
    37141 gtatcagcat tcttaccggc cttgcagcca aagagattgt tataggctcc atgggcgtgc
    37201 tataccaggc agaccaggag gccgatgaaa actcaggcag tttgattgag aaactacaac
    37261 aacaggagca tagcagtggc gagctgaaag gccagaaagt gtttacacct ttggtcgctt
    37321 ttgggtttat gttgtttgtg ctcatttatt ttccttgtgt agctgttatt gcagctatta
    37381 agaaggagtc gagctgggga tgggctgtat ttacaatgtt ttataccaca gcaattgctt
    37441 ggttagtagc atttgcaaat tatcagattg gaagtttgtt ttaatattat aaagttcttt
    37501 aattaagtga ctacacccta ttaattttga ttactgtttg ttcagtgatg caaatcccct
    37561 cctttataag gaggggtagg ggtggttttt attaaatttt aaaaaaatat gatacaaaac
    37621 attattgtct taatgattgt ttttactaca gtagcctata ttatttatgc cattgggcgt
    37681 tcatttaaaa caaagaaaac atgtaattgc gatggatgca gcggctgtga cttaaaagca
    37741 atagtaaaag ctaaagaatt acgaacattg aaaatagtca aaaataaata gaaatggata
    37801 aaaagtattt taaatatgct ttatggttag ctgtatttac ggtggcttat aacataattg
    37861 aaggtgtggt gtcggtttgg ttaggggcag gagatgaaac ccttgcattg cttggctttg
    37921 gagtggacag ttttattgaa gttgtttctg gtataggaat cttgcaaatg atactccgta
    37981 ttcagaataa tcccgacaaa ccacgaggtc gcttcgagat aaccgctttg cgcataacgg
    38041 gttggggttt ttatgtactt gctgttggtt tatttgttgg ggctgcactt aatatttatc
    38101 agggacataa gcctgaaacc acattttggg gctttgtgat ttctgcaatc tccattattg
    38161 tgatgctttg gttgtataag tcgaaggtaa aatatggtaa aaagctcaat tcagaggcta
    38221 ttatagcaga tggccgatgc accttggttt gcatttatat gtctgtaata cttctaatat
    38281 caagtattgt atatgaattt accggctttg gctggattga tgcccttggc gctattggcc
    38341 ttgcatggtt ttcctattct gaaggaaaag aagccatgga aaaagctaaa ggaaaagaat
    38401 gctgctgttg cgggcatgat aattgttaag ctctaatata tttctacatt tggagaaata
    38461 caattctttc atttcttttg tatttcaatt agtcattcgt aattcgtcat tgaatttatg
    38521 cccatcaccg aaaaaaccga aacggaattt gaatttcgta ccagccgcag ctctggtgcc
    38581 ggaggacaga atgtcaataa ggtagagaca agggttgaac tatggttcaa tattctgcaa
    38641 tccacatctt tgacagataa tgaaaaaatg ttgatttcag agcgtttgtc tgccaaactt
    38701 actaaagacg gctggcttcg ggtggtttcg caaaatgagc gttcgcaact ggccaataaa
    38761 aagcgggcaa ttgccaagtt ttatcatatc atggagcagg cattatgggt tgatgttacc
    38821 cgggttgcta cccgtcctac acgtgcttct aaattgaaaa aaaaggaagc aaagatcaaa
    38881 cactcctcag taaagaaggc tcgttcaaaa gattggagta aggaaaggga ataaacagca
    38941 gttagttatt gcttttcttt tctctccttt acaaggaggg gttggggtgg tatttaataa
    39001 ttcactattt ttgctccttc aaaatttata aaaaccttgt cattcaatcc gtttagcata
    39061 gatttgccta ttacagaggt tttaaaagct gtaaaagatt cgctttcaaa agaaaatacg
    39121 cttattgtta gtgctcctcc aggggcaggg aaaagtacat tgctcccttt ggccttgttg
    39181 gatgaacctt ggcttgaagg ccgtaagata atcatgcttg agccccgtcg tttggcagct
    39241 cgcaccattg cagcccgcat ggcttcgctt ttgggtgaag aagttgggca aacggtcggt
    39301 taccgcatac ggtttgacaa ttgcaccagc gaaaagaccc gtatagaggt cattaccgaa
    39361 gggatactct cgcagatgat tatttcggac aatacactcg aaggggtagg ggcggtgctg
    39421 tttgatgagt ttcacgaaag gagcatattt gcagatgttg ccatggcgct ttgccgcgag
    39481 acacaaaata tcctgcgtcc cgatttgcgt atggttgtca tgtctgctac actcgatatt
    39541 ccaaactttc aaacagtact caaagctccg gttattgaga gtaaagggaa acaatttccg
    39601 gtagagattg tctatactgg cacacacgac ttgatgatgc tccccgaact tgctgcccgg
    39661 actgttgcaa aagctgtgtc ttcgcacccg ggtgatgcgt tggtgttttt gcccggtgaa
    39721 gctgaaattc gcaagtgcga agagatcctg aaaggtgaac ttcgggattt tgccattcat
    39781 cctttgtacg gacagcttac ccagtcggaa caaaacagcg ctattgttcc caataaattt
    39841 ggaaaacgta agattgtgct ggcaacttcc attgccgaaa cgagtttgac cattcagggg
    39901 attaaaatag tcgtggattc tggctttggc cgtacttcaa agtttgaccc taagactggt
    39961 ctgacacgtt tagaaactgt tgctatctca aaagattctg ccgatcagcg tgccggacgt
    40021 gcagggcgtt taagtgccgg cgtttgttac cgcatgtggt ctaaagctac gcaggaaaac
    40081 atgcgagggc atcgtgtccc cgaaatcatg gaagctgacc ttacttcgct ggtactcgat
    40141 atggctgagt ttggcattat tgacatacag cagttgacat ggcttactcc tcctcctaaa
    40201 agtgccatat cgcaggctac agagttattg cacgaactgg aggctttgga acatggccgt
    40261 attaccgaac aaggacgcaa gatgcaccgg ttgccatgcc acccgcgtat tgcccacctg
    40321 ctgttaaagg ccgaagaaac gcatttgctt gccctggcta ctgatttggc cgctgtgctc
    40381 gaagaacgtg acccgctttc gcgtgatgca ggtattgata ttaatgtacg tattgaaaca
    40441 ctaaggagac agcgtagtca gcaacgcatt caaagaaatt ttgcgcgtat cgaacaggtt
    40501 gcttctactt atcgcaggtt gttcaatatt gaggcggata atgattcgca taacccttac
    40561 gatacaggct ttttattaac ctttgcttat cccgaacgta ttgcctgtgc ccgcccgggt
    40621 aacaatgccc agtttcagtt gtccaacggg gctattgcta tggccgggca taaggacgac
    40681 ctggctcacg agtcgtggct ggctgtggca cacctcgatg cccgtgatgg catggggaag
    40741 atattccttg cagccccgct taatcccaaa gaccttgccc ctttggtaaa ggaacaggag
    40801 gttatcactt gggatacccg taaaggtggg cttgtagcta ctaaagacct taaaataggt
    40861 aatattgtcc ttcaatcaaa accattgcct gagcctgacg aagaacataa agtacttgcc
    40921 atatcggagg ctataaaaaa ggaaggagag gcgttgctca acttcgacga tgcttttacc
    40981 aattggcaaa accgagtgtt aagccttcgc aaatggcgtc cgcaggaagc ctggcctgat
    41041 gtaagcacaa caacactgct tgtgaccaac tatacatggc ttgcccctca tttgtcgcag
    41101 gtgaaaaaga ccgaggattt gaaaaaaatg aatttggtag aaattcttca ggctcagctc
    41161 gactgggaaa aacaggctgc gcttgataag cttgccccaa ccaggataga agtcccaagc
    41221 ggttcaaaag ttgccatcca gtatcaggcc aatggcgaca accctatcct tgcagtgcgc
    41281 ttgcaggaag tgtttggcct ggccgatact ccgcttatca acgaaggccg gacacctttg
    41341 gtcttgcacc tcttatctcc cggatttaaa ccggtacaga taacctctga cttacgaagc
    41401 ttctggaaca atgcttattt cgaggtaaag aaagacctcc gtgccaggta ccccaagcat
    41461 tcatggcccg acgacccttg gacagcccaa gcgttgaggg gaataaaaaa gaaataaaac
    41521 tttgtttact tgttttatga agtcaacttc ttttgatttg aggaaaaatt tctaacgttg
    41581 tattcatttt tctttttcaa tgaaatgctt taatgctctt cttagcgctt cattaaaaaa
    41641 agtccaatca tggttgcctt gccagtcttc aaaaacagga tcatatccta agctttgcaa
    41701 tgtagcaaaa taccttttgt tatcaccata aaaaaagtct tctgttccgc aggttatata
    41761 taacttaggc tttgttgttt tatcttttgt ttctttggcc agttctatca cgtcttcacg
    41821 cggatcccat tcaatatttt cgccaaatac agcctgaaag tctttaaaca aggcttcacc
    41881 aaacagttct ttgaatcttt tggctccttc gcaatactca agagattctt tgagcattaa
    41941 acagggagaa gaaaaagctc cgcaaaaacc atattgctca ggttttgata aggtacattt
    42001 caaagctccg tatcctccca ttgaagctcc aataatggct atgtcttctt tttttgccga
    42061 tattttaaaa atactgttgc acattaaagg gagctcttcg gtgatgtaac tgaaataatt
    42121 caggccgtac ttcatgtccg aatagaaact gcgggctact tcaggcataa caaatattgc
    42181 atgatattct tttgagtaat tgggcaacag ggtatagtca acccaatctc cgcttcttcc
    42241 gcatatccca tgcaacagat atactacttt atagggctga cctgaaaaat tatcaggagc
    42301 tacaaaggta atattggttt ctatctccaa tttacctgaa aaaaaggtgc ctcgtaaaat
    42361 catattgttt cgattaatat tttacttatt attagactta caaaaatatt tatagttcac
    42421 acttgctttt agtgttaccc ttattcttaa aagtaaattt cttataattc tttttttgac
    42481 aagtttaaat atattgaaat acaattacat atagttaaaa tatgaaggta aaaagcaacg
    42541 tagttgctac ctctttgcag tcaataaaat tgccatagtc atcaggtgtg cagcaccgat
    42601 atagcatata atatatttcg ccactacgtg gcttaatatt tacgaacaac atttttctat
    42661 aaagatttcg ccgctaagcg gctaatagca agggttaact aatctgtcaa agtagaatta
    42721 agtattttct aatatttaat tagggttaaa gtaaaattga gctgttggta tcatgtttaa
    42781 aaatgcttaa caaatgattg tgtttatgaa agactctgca acccaggaac aaaacaaaaa
    42841 aagcggaacc tattgagctc cgcttttttg ctttataaga tgataagatt atttcttaac
    42901 aactttttga gttgtagagc cattagcagt ttggattttt acaaaataaa ccccattttt
    42961 caatcctgaa aggttgagtt caacgctatt gccattaaca ctggcaatac ggttaagttg
    43021 gccagacatg ctgtatatag caactgtgct gatggtttcg ttactgcgaa tgtttaatac
    43081 atctacaaca gggttagggt aaaccgaaag acttgcaata gaagcattgt taatgctggt
    43141 ttgacctgtg atagtaacag taaatgtagc ttttacgcca gaagcatcag tagctgtggc
    43201 tgttactact actgtaccat tagcaacagc agtcaatact cctgtttttg catcaatagt
    43261 agcaatggcc gtattgtcaa cactccattt aacagcagtg gttgtcgcat tggtaggagt
    43321 tactttagct gttaaggttg tagttccttt ggctgttgta atagaagact caccgctgat
    43381 ttcgatcttt gttacaagaa caggagtgaa gaattccatt ttaccaatgt aaacaacata
    43441 gcctttaaga gctgaccagt tagctgttga ttgggctgca agctgaagca tgctaaatgt
    43501 tgtatctact aaagttgatg tcaactcctt aatggttgta gaagcaggat tgtcggtaat
    43561 agctttgttt ttcttgaaat cacccggggc taactgaata aatgcctgaa ctgcatcagt
    43621 tttggtaaca gtgtctttgt catatttgta agagaatttc actgttgagt atgacttagc
    43681 aactaccggt tcgatggcaa atgctgtgcc ccaaccgtta accctgattt ttaagtaatc
    43741 tttgttgtta attttaacaa tctcgcctcc gtcaggcact ttgccttcaa atgtagtcat
    43801 gttgaaagga ttggtgaatt ccatctggct gatgtaaaca acatagcctt taagggcaga
    43861 ccagttagct gttgattgag ctgcaatctg aaggtttaca atcgtatcag cttgtaattt
    43921 agctgtaacc gttttgaaag ttgtagaagc agggttttca gtaaatgctt ttttgcttac
    43981 aaaaccgcta tcggcaaatt ggataaaggc ttgaacaaca tcagttttag taactgtgtc
    44041 tttatcgtat ttgtaagtaa atttagcggt agaatttgaa taagtaacaa caggttcaac
    44101 agctattgtt gtattccagc catttacgcg tactttcaaa tattttttac cttcaatggt
    44161 atcaatagta gctccggcag gaaggtcgcc tgcaaatgta gctaagttga acaatacctg
    44221 gttagaagcg gtaattctga tagagtcttt ctttgctcct tcattgctgg taacataaac
    44281 cataaagctg ccatttttgg taccattggc ttttacaaca cctttattgc taactgtagc
    44341 aatagtagca tcgcttacac tccatgttac gttggtatta gttgcgtatg atggataaac
    44401 attagccttt aactcaatag aaccttttct tgtggtaata gtagtgtcat tatcaaaggt
    44461 tagtttaaca ctgtcaacaa acacttcctg gtaaacgata ataatggttt tttcggcttt
    44521 aatagtactg ttacctgcag ctacaacttt aagtatggca tagccgttgg ctaaagatgt
    44581 gattgtagaa tctgccgcat tgatagatgc tgtagtaact gcattaccgg tgctgtcaac
    44641 taaacttgaa atataagtta atgtatcagc atcggcaggg cttatagaag caaaaaattt
    44701 ggccgggcca ttagccgctc ttatggtgtc cggggctgta atagtaatac ctgcaacagg
    44761 tacaacctgg ttgctgatag caatgtcttg tgtagcagag aaacctccgt caacagaaac
    44821 agcttttact ttaactgttg cattacgaac tgctgtaagc aaccctgtag ctgagatagt
    44881 agcagtatcg gcgttttgat taaccaggct ccatgttaca cttttgttac ttgcatcttc
    44941 aggggtaaac acagcttcca tttgcaatgt gccgttgtcg gttgtgatag tgtttacatt
    45001 gttagcgccc ctaattgtca atgcgttaac tggtttgtaa gttgaaccat ctccggcaat
    45061 aaaggttata gagtcaatat agaaggtata acctaatgag tcggcattag gaggggtaac
    45121 cctgctgtat gttgcttttg gagaattcat tttgatatag aactggccat cagcagccat
    45181 agcagggtca ataacatact cgtactctgt ccatgtggct ttggtgattc ctgtagcggt
    45241 gtatttttca gcaatactgt tattgatgtt cattgcaaat tggaaatcaa cataagcaga
    45301 gtctgaatat actttaaact taatcctgtt gtatttgttg aggtcaatag ccggtgtctt
    45361 aactgctgca gcataacggt tgcatttttc tactttcagt actttgtctg atgaatttcc
    45421 tactttatta gggttgtcaa ctatcgtgac agttggggca ggtgtgccag cgccattcca
    45481 tccgccctgg gtttggttag gattgatacc gttttcaaaa ttggcctgta ccattgatgt
    45541 ccctacaaca gggtcagata ttgtaaaaaa cagggtgtcg cttacgttgg catctaatgt
    45601 gctggtggca taaattttca cagcgccggc ctttagtgca gttactacac cggcagaggt
    45661 aactgttgca atagtggcat ctgaggtacc ataggtaact tgcgaacctg ctgtggctgg
    45721 ttctactgtt aagcttgttg ttattttttt acctacaata ggcgttaatg ttgcagcaga
    45781 taaagtgatt ttggtaggtg gggttgatgg cttggctaca atttttatat tgtcgaaaag
    45841 taagtttgta gcaacagtat tattctgaag ggcaatttgt gttattgaaa caaatgtacc
    45901 aaagttggta gaattggtca agtccacttc ataatgtttc caagccccgg cattagtaga
    45961 tgggtagaaa ccctttgaaa catctgaatt actggcattt ttgccatata cattgacaat
    46021 gaaatttact gctccggtag ggtcgtaaac atctaaagtt actttttggt aatctgcgat
    46081 gtttacattt aatccacctt tggcaacacc ttgatattgg gctgttgtta caacctgcaa
    46141 taccttgtca gaattgttgt ctgtgttttt tagaggattg ctggcatttg taactgttcc
    46201 accccaagtt acccatgagt tgacagttcc atcctcaaaa tcgcccagat tgatatctgc
    46261 ggcagcttgc gcgaagcttg tagcgataaa aaacgctacc agaaaaataa atagtttttt
    46321 cataattaat ttgtttgttt atgcctttcg gcttagttag tgaataagta tttagttgaa
    46381 taaaaaacat agtgtatgtg tgagtacgag tatttcttag tttgtatttt tagaaaactt
    46441 tcttttatct aaacgaacca aaaatatgaa ttaaagaccc taaaggttat gaacaattat
    46501 tgcacaaagg aggattattc tgtcatattg acaattatat tattattcaa gggataggat
    46561 aggaatagga atagtgtttt atgttaacga actgttttaa tgcatttaac aaagctaatt
    46621 ctactttgac agattagtta acccttgcta ttagccgctt agcggcaaaa tctttgtaga
    46681 aaagtgttgc tcgtaaatat taagctacct agtagcgaaa tatattatat gctatatcgg
    46741 tgctgcacac ctgatgacta tggcaatttt attgactgca aagaggtagc aactacgttg
    46801 cttttttact ttcatattta tatataattg aatttcaata tatttaaaaa gtagaactaa
    46861 ttttttattg ttttagttat tgtattttca aaaaaatcgt ggttgacatt ttgccttttt
    46921 taaacaactt ctgtaaaata tgaaagtgta tttgttaaaa atactataat gtaaaatgca
    46981 caatttcaac aagatttacc tccggttgat agtgtggtgt tatctaattt agtatattta
    47041 tattgactaa acctaaaacg ctcatggaaa taaaatatca actttttgag gctgaaaact
    47101 tattggtaca aaagtacagc ggccaatttt caatggaaga atttgcggct tatgcccgtt
    47161 ttagcgggat gaattttgct tcaaaaccaa taaaaaaggt tttgctcgat tttagagact
    47221 tgcacatggg taactacgaa gatttcaacg aaacgctgga taaagtcatc aaggtgcgag
    47281 aacaggtcaa cgaaaatgag tttaaaaaca gggcagtgac ttttgttatt tgggtcgaaa
    47341 aacccatgcc tactgctgtg gcacatcttt ttacggccaa cttttctggc aaaaattatc
    47401 actatgccct cactgtcgat agtattgttg atatattgaa actcccgcaa caccttagga
    47461 atgtagagaa gattgttgaa aatgccgaaa atacttttta tgcggacaat actaaaattt
    47521 aaagcttgga tgcgccccac aatggctttt taaaagattg agataaaatt tattattgag
    47581 ctggcgtttg tttaaaactt ataccccatt cccagcccca catgaggttg aaaaaagtat
    47641 ttatcatcct gcaataaagg atcctgaaga caggctaccc caaaaacaac ctgccacatt
    47701 aagccaaagt gcgctgttcc tttatatccg gcaataaatg acagcccatc caaagatttc
    47761 ttgtaaatta cagcattttc agggtcattg gaaatatttc tggctcccac tatctgagga
    47821 cctaatataa acctgtgcat tctgcccatt tcaagattaa cgccaatagc ataactgtac
    47881 caaacgtatt cagcacttga tgagtctgag catcctacag agaatccact accgacgtaa
    47941 ggtgttatac gaacattatc tttaaaacgt aactgatatt caatcattcc acccatacct
    48001 ccatacaaaa ccccattccc tacttggatt gcaaaccctc ctttggtggt tttatcttcc
    48061 tgctgggcag ataaaagaga gaaggtaaaa agcaatatga caacccaacg cttcatcttg
    48121 ccagagttta gttttactgc taatttccta taaatttacg aaaattcagg gcttaaaaca
    48181 aggtaaaaga caaataaata cgtggatttg aaagttttct aaaaataaaa aattgagaaa
    48241 ataggtttca gaaaacagct aaatcacttc cgaaaccttt acacaatggt attcggtaag
    48301 aaacccaatg gaaagaagat aaataatagc aaataaacct tacatttgat aatacatcat
    48361 aatggtatta acctaatgac tacctatcat aattaaatcc gctacttcgc atgaagcgct
    48421 tgcgattaag tcattaggcg ttatttttat ttgcaatccc cgtattccgg cactcacaaa
    48481 aatgtaatcc tgcaaaatac agctctcatc aatatatgtc gggaatttct ttttcatccc
    48541 caacggtgaa cagcctccgc gaatgtaacc ggtaatcccc agtatttctt tttggggaac
    48601 catagcggca cttttgttgc ctgaaacatg ggcagccttt ttaaggtcga gttcttcgcc
    48661 cccgggaatg atacaaacga atactcccgt tttatcacct tgcaaaacaa gggttttaaa
    48721 cacctgttcg atattttgtc ccagctttga agccactgca atagcgctta aatctgattc
    48781 gtcaacggtg tattctacta aatcgtactt tatgccctga acatccaaca agcgggcagc
    48841 attggtcttt gtcatatctc ccccttttcg aacaaatcaa taagttcgcc tgctatacta
    48901 atattattgg gacgattggg cagattgcca cgtgtaaacc aagctgcatg ggtaatttct
    48961 ttattatcaa tgatgattgg ttggttacaa tcggcctctg caaagaaccc tatcatcatt
    49021 gaccctgaaa caggccaagg ctggcttttg taataacgaa ggttggtaat atcaaggccc
    49081 acttcttcct ttacttcacg taccacagtc tgctccaacg actcgccaac atctgcatac
    49141 ccggcaatga tagaatacca atcggcacgg aagttggcat ttttggccat cagtatcttg
    49201 tcttcacaag tgatggcaac aatgatggcc ggagatattt taggaaatac caaatgatta
    49261 caatgagggc acataattgc cctctcgtcg ggtttctcga tggttttact gccacacttc
    49321 ccacaataac ggttttctaa gtaccagttt ttcatctgat gccctacaat gctcacccag
    49381 gctatttctt tttgaggaaa atcccgaaac aggttcgatt cctggtaaga aagttgccca
    49441 ttgacctttt tggggtcatc ccaaagaaga aaacaaggca cattgtcgta agtgaacaga
    49501 aatgtacttg ctgaagtgtc gtatatttcg ttgacatctt tctttcgggg cagttcaaag
    49561 ccatgtccgt tttgcttcaa cagcaacgaa ttctggctgt aatgaagaat gtaatccgac
    49621 tcttcaatat gtttggtcgc aataaactgg ttgttgaact gatggggaaa tatatcgtta
    49681 atcatgctgc gaatatttaa aactatcacc atttacagtg attaggccat gcaaagatat
    49741 acagattagt gttttgttgg aacaaccatg ttttattagc acaaaaaaag caaggcacgt
    49801 tttatattgc cctgcttttt gataattaaa ctaattggtg cttatttgtt ttcagaaacg
    49861 ttcatttcgc actttttcat tggcatttga gcgtgttcca tacggttatg ccttatttgc
    49921 atcccttttt tccctggttt gtgcttgcgt gagtcaaata tcatgcgttg ttcatctgtc
    49981 agaagtgccc ttatctgctg gatatgtgca gctcgggctt tcatcaacgt attttcgagc
    50041 ttggttattt catcaatgtt ggcattgatg gctttcatgt cgggtttttc agcagtggtc
    50101 agggtatgtt gtttggcttt cagctccttt agctggtttt tgaccggctg cacttctttg
    50161 taatgggcaa gtttcaactc tttgagtttg gcagactgct cagtggtcaa cccggggatg
    50221 cccatttcat tgttgaaagg acacaaagcg gctttttcgc atttcatagg tgggttttcc
    50281 tggttgggct gagctaccat gtttacgcca ataaacatgg ccaatgcagt aatgataatc
    50341 agattttttg ttctcatcat aaaaaatttt taaatgttat tgctgttttt aatacttaat
    50401 actttttaga cagtcgtcaa acaaaaatct ttttccagcg aagcactatt ttcatttatt
    50461 taagtaattt tcacttttgt aaaattcata ttaatcagat ggaattgaac ttttacagaa
    50521 aatagaaaca actaatactt tatgatgatg aaaacaatta attggaaaaa gtttattcgc
    50581 aaagcaagtg ttgcattatt gcttattacg gttgtcatgc tcatggcttc tgttttggct
    50641 atggaaacta tgggtaaaag tcacgattta gaagaattgc atgagttttt tggtttcacg
    50701 tttttaattc tggttatggt gcatatcgtt gcgtttcgta aaagcctgaa aaacactttt
    50761 tttgtgtaaa aaatactgtt gtctgataaa aattttgtac taaaagtata ttgatatgtt
    50821 tgggtttttg ccctaatgta tttttatatt ggccattaaa cttaatttat agtgtctttt
    50881 tattggtttg ttgacaaaaa taaaaaggga cgcaacctat tatggtttat tatcatctaa
    50941 gcagaaattt aagatataaa acactcatgt aatagaaaaa caaacattta aggataaatt
    51001 gctttcatga gaatttcgat tgaataagct atccctttaa aaattaaaat ttagtatgat
    51061 tcgttaaaca caaacaagga cgacccgatt agtcgggacg ggttttcttt cggagtgccc
    51121 ttcggggttt atctgatctt aataaaattt aaaaaattat ttacggatga aaactttgat
    51181 aactttattt attacggcat cgctgattgg atttgcaatt tacagttgcg aaaaggacaa
    51241 agctaatacc ccacaatcaa cgtttaaggg tcaacttata agtaagtctg cttgtaaaag
    51301 ttttttaaaa tctacaagag tgattactga tattactaat actcctgata gtttagtttg
    51361 tataggatat tcatttgaca attcaactaa taagctgtca attaaacata ttaacgctgg
    51421 gtttaattgt tgtcaagata ctgtttattg taatgtttca ttaagtgttg atacaatact
    51481 gattcaggaa atcgagcaaa accccaaatg tgattgtgat tgcttgtatg atttaaacat
    51541 cgaaattcag ggtgttgacg ctaaaaaata tcagattaaa gtgattgagc cgtatgttgg
    51601 tgatcaaaag aaaatattat ttgagattga tttgacaaaa gataaagtgg gttcattttg
    51661 cgtgacaaga aaacagtatc cttggggtat gaaaagtctt aacaattaac ttttgcctac
    51721 aaccaagctc atgttttgtg acaaaaatat ggaatatgtg gtcggacccg gcgattttga
    51781 ggtcatggat ggacgttcgt cagatgacaa cttgcaaatg gctacgttta cagtattgta
    51841 aatagttgtt actggttaaa agttattctt tgctgtattg actcaatgcc aaatagttcc
    51901 tgttttagca ttattgtttt ataaatttct ttttaattcc acttacgtta atcatataaa
    51961 cacctgaatt aagagaatgt acatctacca ttgtttttcc tgagaaattt tcttcgtaca
    52021 ccatttttcc tgttaaatcg tatatttgta cataatgcgg ctttccatca gcaatgtcaa
    52081 tagtaataaa cgttgtagca ggatttggct taatggttat tttatttaca ttttcagcaa
    52141 tggtaggtat ttctttagct ttaacaacta tgttaaattc tttagacaga gtgtctggac
    52201 caatatgaac aagtgctttt attgttacat tttttgaata gttaaatggg cgtgttacta
    52261 ttcctgagtt tgatacaata gaagtgtcgt ccgattgcca gataattttt gtaccttctc
    52321 caagagtgct ggtcagttgt aaatttgaga caataccagt agagtcttca ttagaagcaa
    52381 tacccaatgc cgatatcgtc atgctgttta ttaatctttg taatgtttcc aatctggatt
    52441 cgggtttaac taaaacctta taacctttgc ataatactgt atcttcaagt ggtaccgtgg
    52501 caattaaatc aacgcaggta tctttatgca tccaattgaa cctctttata tttccatcag
    52561 gaagtaacaa ggatttatct ttggtatccc atgaaattga aaattggttg ttataaaaac
    52621 tcttggccaa agcatacttt gaaaaggcaa tattatctga atagtttgta actcctaaaa
    52681 ttttactctt gtcagacaat gaatccaaaa caatttgata ataatattgc cttgcattca
    52741 gtactaccaa ccatcttgaa acttctttgg cagctttgta aattgaatta ccttcctgac
    52801 gggctgtaac ttttgtgata ccgggtattc cttttaggat gcatttagag cctgaattaa
    52861 aagcaatatt ggcattttca atgctaaaat ttacatccag tcctgaagtt gcaaaagctt
    52921 ttaaatcaaa agcactatcg tttacagatt tatatagaat tgaatcaaac gtaacagatt
    52981 gcggtaagcc cttattgcta aatgaaaccg ggatttttcc atctgtataa ccaatgtgcc
    53041 agcttttttc atggtttgcc gaataccata ctttataata atatcccgaa tcggtccggt
    53101 atgattgaaa tgtagggcgg tataattttg tatcccattg tctgtaatta atggcattgt
    53161 taaggatagg cgttttaaac tcaaggtcta ttatgttggg ggtatccatg tggcattgtg
    53221 caaaaatgat acatgccacg ttatatacat tttcgtaact acttgtgcgt gcggcaatca
    53281 taaattctat tcttttctca aaatagttgg gtttgcacgc aatatgccag ctataaaaac
    53341 cactagaatc taaagcatat ttgttgctac tgtttttaca taaggtttct tcttcccaat
    53401 gtatcccatc gctactaaaa caatggacta aacggttggg tttcaaaatg taaacacccc
    53461 agaaaagcca tttattagat gcttctcgcc aaaaacatgg tgatacaatt gttgcaatat
    53521 cgcgtggttt atataatttt acagtttctg gttcagttga ctctccattt tcttttatcc
    53581 gtatcagttt tatggctcca tatattgaat cttttccaac aataatcagc ccccggtaca
    53641 tataatataa ccagatttcg tttacatcag gattataaac tatatcaaca tcgcaattat
    53701 tgcctgttgg aggagtgcct gttaaaggaa acttaaaccc cgtggggatt atccaattct
    53761 tgccgtcatt gcttgcggct atacatgggt tttctactaa tgcgtttgta ttatagtatg
    53821 gagtataggc catccagtaa cggtaacctc cccatgtccc acttgtgttg acttctttct
    53881 gaaaatcaat aattgacggg tgcacagctt ctgaagttga atccggggtc ggaaattgca
    53941 ttggaaattc ggcattttgc aatatggatg atgcccctaa tgcttttgta ctttttaaat
    54001 ttgaaattgt aaattttctg aaattatcaa tagtgtcagg gttgagttgt gcttgatttt
    54061 tgttgtttaa ccctattaaa aaaaatgata gtagtaattg acagtaaaat aagcgtctca
    54121 ttaaaaatct gttttaaatt tctttttgct aatatatttt aagaaaacag tctttttgta
    54181 ttcccccttt gtttctaatt tttttctaat ctgcgtttaa taccattcta attcctcaat
    54241 gatttctttg tgacaaacta taaattacat gtgttatgga attggagaga aagaaacggt
    54301 ttactttggc aattttactt gccctttcat tagtttttag tgggatcttg ttttacatgt
    54361 ataggtcttt aaagcatgat tgctcaatag acaaatctgt tttaactacc gaaaaatcaa
    54421 tgttaaagca aagcaataac gaactggctg ctgaaaagcg ggccctgtgt gatgagaact
    54481 ttgaacagaa agaaaaaatt gaaaggatga tggaaacttt caagcctgaa aagaagttta
    54541 ctaaaggcat ttataaattt tccattatga aaggtactga gactgtttca accatgcagt
    54601 ttcagctaaa ataacggttg tgttctgttg cccggagatt ggcataattt tgcctcttcg
    54661 ggcttttttt aattttttgt aaagccatga aaaagtcaat tacccttata ttaagtttag
    54721 catttgtcag tttttatgca acatcccagt cattgtataa gattggccat atcaacaccc
    54781 aggagttaat agaattaatg cctgaaacag acagtgctca aaagaaaatc gacaagcttt
    54841 ataaagacct tcaacagcaa ctggaactca tgcagactga gttaaaggtg aaataccagg
    54901 actatatcaa caaaaaaaat acattctcgg agtccgtttt agagcttaaa gaaactgaat
    54961 tacaagacct taatgcccgc attcaaaaat ttcaaacctt ggcacaagat gaggttgaga
    55021 ataagcgaaa cgatttgtat aaacccatac tcgacaaagc caatgccgca atatctgcgg
    55081 ttgctaaaga aaatagcttt acctacatat ttgatgcaag cggaggggct ttgctatata
    55141 aagctgataa cagcatagac ataatgccgt tagtgaaaca aaagttgaaa ttacgttgat
    55201 ctatttttat aactttatca aaaacatatt tttcctatga atactatatc gcttaaactc
    55261 ttctttattg aatttcaaag aaatgagata accgatcacc ttatttattg ccgcttaagc
    55321 aatttaacaa aagactattc gctcaaagaa cttttagaaa aaattgctga tgacgagatg
    55381 aaacattact ccttttggaa agaatacacc aataaagatg taaaaccata ttattttaag
    55441 ctgttttggt acggttatat tatgccattt gtaagtaaaa agttggccct gcggtttatg
    55501 aaacggctcg aaaactatgc ccaccttgaa tatacagcca atcttctcaa tgaagttgaa
    55561 gggcaatgga ttattaatga tgaaagcttg cacgaaaaaa tgttaaacca ggtgttggaa
    55621 aatgattatt tatccaaaac tgtcgaagaa taaaatactt agttttgacg tatgacagtc
    55681 ggaaaaaaaa tactaattgt tgaagatgaa aaaaggcttg ccgatatcct gaaaaccggt
    55741 tttgacgaaa atggttttgc tgttgaaatt gcttatgatg ggtatattgg caaagaaatg
    55801 ttttcaaaaa acaaatatga ccttgtcatc ctggatatta acctgccttt aataaatggc
    55861 tatgagctgt gcaaagctat tcgtatagtt aataataatg tacccattat catgctcacg
    55921 gccatggggg ctgctgagaa caaattaaca ggttttgagg taggggctga tgactatgtg
    55981 acaaaacctt ttaattttaa agagctactg gccagggtga gagtattttt aaaacgttct
    56041 tcaaccattg aggtggtaaa tgatgagatg atacaaattg acgatttaat tattaataca
    56101 agtaaaaaaa cagtctttag aggaaatacc cagattgaac ttacggcaaa agaatattca
    56161 ttacttgcat acctggcaaa aaataaagga attgtaatat ccagaactga tatagctgag
    56221 aaaatctggg ggttgaaatt tgacaccggg acaaactata ttgacgttta tattaattat
    56281 ttaagaagaa aaattgatta tggtttttcg aataaactca ttcatacaag agtaggttta
    56341 ggttatgtgc taaaaaatga aaatgaatag ccatgaatat tcgccttaaa ctctccatcc
    56401 agttttcatt gattgtaata tccattttat tggtattcac tacaagtata tattactttt
    56461 cttcttcata tcgcaaaaat gattattaca acaggattaa agacagggca tatacaacag
    56521 cacgcctgct tcttgatgtc caagaggttg ataaatctct ccttaaaatt atcgacaaaa
    56581 atacacaagc cctctaccag gaaaaaatcc tgttttatga ccaaaacaat gaattggtgt
    56641 atagtaccac tgatgattcg gttaaaataa accttccttt aattaatgaa ataagggcag
    56701 ctcaaactgt tgaaactaat gatagccttc gcgaagtttt aggttttctt tacaaataca
    56761 ataatcagga atatgttgtt atagcttcag cttatgacat ctatggattg gctaaaatca
    56821 aaaacctaaa atttatttta attgtcgggt tcttaatcag cataataatt accattatag
    56881 caggcttaat attttctggc cgtgccctta aaccaatgtc taaagtaatt aaccaggtga
    56941 aaaaaattac catcactaac ctgaattcaa gggttgatga aggaaattgt aaagacgaga
    57001 tagctcagtt agcaattact tttaatcaaa tgttgcagcg gcttgaagag gcttttattc
    57061 ttcagcgtga ttttgtttct aatgcagctc acgagctgcg tacacctttt actgtcatgc
    57121 tcgccgaagt tgaatacaac cttttacagg agaggactaa agcccaatat gttaatacgt
    57181 tgactaattt ggccattgaa attagaaaaa ttagtaaact ttccaatgga ctgctcgacc
    57241 ttgccaggat aagttatgat aattctgctt ttgaaattaa cacaatacgg atagatgaac
    57301 ttttattaga aatttgtaat gatatttctg acattaaaaa agaacaccat accaacatta
    57361 cttttaaatc gttgcccgaa gatgataacg atttgaatat ccttggaaat gcacaacttt
    57421 tatctatcgc ttttaaaaac attattgaaa acgcaagtaa gttttcaaac tataatgttg
    57481 tcgatattga tttttattcg ttaggtaaaa atatactcat tcagtttaaa gataaaggga
    57541 taggcatttc accagatgat atacataaga tatttcaacc tttttaccgt ggcaaaaata
    57601 cgcaatttat tgcaggttat gggcttgggc tggcattaac cctcaaaatt atccagctac
    57661 ataacgggac aattgatatt acttctgaat taggcgttgg aactactgta actgttactt
    57721 tgcccaacaa agcaagtttt atttaatact catctgacaa tattattttt ttgtgtataa
    57781 aaaaagggca gtaatcaaaa ttactgcccc ctaacacaaa tttacctaga aaacccttta
    57841 aaggattcgt atttctttgt caaatttacc tgtaataaac cttgattaca atcttttctt
    57901 attagaataa gattagaata cattgttgtc tggtacaaac aggtcgccgc tatgcagctt
    57961 aggaacattt ggtgtattgg gttatcctac aaactggtcg cagctacact gctattattt
    58021 ggtcgcgtag cgacgttttg tttgtagtaa tttaaacatc gttaaaataa agccccggcg
    58081 gggcgaccag tttctaatca acaatataac tagtatttat tgtatctgtt ttattttacc
    58141 ggacaacatt gattagaatg tttaaaacat agaatgtttt atatgagatt ctgagtctga
    58201 ttacttttaa tcatgggtac tcataatgtt agcgtttatt ttttctaaca tcttccaaag
    58261 ggttttcaat tcggccggcc cgtctgtcat taaatcaaac attactttgt gcctgtcatt
    58321 tctggttttt actattatcc atccggcacc tccatcgcag cagtcggggc atccatagat
    58381 ggtgtcgagt tgggcaaaat tgattttttt tacttcatta acaatactgt cccattttgc
    58441 tgtagtaatg tattttgtat attgttttgt agggtatttg tctttatgcc agcttgaaat
    58501 agtaatattt gcagtggtcc cggacagttc aatttcgtga tgacagtatc cataacattc
    58561 tccaaaagaa gttccatagt ttatactaag gctatcgttc atattggtga ggtaagattt
    58621 tttttctttt tcgcatgaaa ccataaacaa ataaatgcaa gcaatcataa tagggataat
    58681 gagcgtggcc cctaaagcga caatagcaat ttttgttttc atacagcagg ttagtttgtg
    58741 tttttataaa gacccgacaa aacaaaaagg ttgcttataa aaacaaaaaa accttccagg
    58801 ttctccggga aggtttttga taagattctc tgagattact tatttgtatg cagtcacagt
    58861 ttcaatagta ccatctggtc tgtgtttaag ttctactact tttatgcagc gcaggcgagt
    58921 ctctccattt gaaagactgc tatcgtgata ataaaggtac catttgcctt taaactcaac
    58981 aactgaatgg tggttggtcc atccaataac aggatttaat aatatccctt tgtaagtaaa
    59041 agggccataa ggattgtcac caatagcgta gcagataaaa tgtgtatcac ctgtagaata
    59101 agtaaaataa tatttaccat tgtatttgta catccaaggg gcttcaaaat acctgcggtt
    59161 gtgatcgccg gcaagaagtg gtttgccatt ttcgtcaagg ataacaacgt cttttggctt
    59221 ctcgtcaaac tcaagcatgt cgtctgtcaa acgggccact ttagcgcata atgcaggctg
    59281 gtcgtcagca ggttcaaggt cgaaaggcgt ttttacattt tcaacaaact tgccagtttg
    59341 ccagcgttga agctgtcctc cccaaatacc tccaaagtaa aggtatgctt tgccatcatc
    59401 atctacaaaa gcgcatgggt ctatgctaaa gcttcctttg atgggctcag gctgtggctt
    59461 aaacggccct gcagggttgt tgcttgttgc taccccaata cggaatgcgc catcttttgc
    59521 ttttgctgga aaataaaaat aatatgtacc atttttgtaa gcagcatcag gggcccacat
    59581 ttgtctttcg gcccatggaa catcctttac atgcaatgct acgccatggt ctttggcttc
    59641 tttgtctgta ggtgaatcca ttgagaatac gtgatagtct tccatgcaga agtgcccgcc
    59701 aaaatcgtct tccggaacat ctacatcaat atcatgtgaa gggtagatgt agattttgcc
    59761 ttcaaaaaca tgagcagaag gatctgctgt gtaaatatgt tttactaaag gttctgaaat
    59821 aggcgttaat tttttttcgt ctgccataat atttaatttt tagtttgaat taatgtattt
    59881 ttttgaattc cccaaaagta taaaaatact tcttttcaac taagaatgaa aggaggaata
    59941 ataagtcaaa aaagggtaac tttttaattt taatctggac gacattagta aatgaaagat
    60001 tattaataga agttattgaa tttcttcaat tatccattct aaattgtcat taatgatgaa
    60061 agatgccgga tacgattgcc agttgccttt tttgtttata atttcgtcta tgataagctt
    60121 tttgtcgctg ccagtgatga taaaacatac ttcattggcg ttgttaatgg tttttccggt
    60181 aagggtaatg cgtttttgac cggtatgagg attgacgctg gtagtataag cctcgtcaag
    60241 gttaaaaagt tcgagttgac cggggaaaat agaggcagtg tgtccgtcgg tccccatgcc
    60301 caaaagtatc aagtcaaaaa ctggcaggtt gtttttttga ggaagtttac tttgtactaa
    60361 tgaatttaat ataaatactt cttcgtgggg cttattatcc cctacaatgg ggaatacatt
    60421 gtcctgggtt gttaaaactt tatcaaaaaa aaggcgttta gcctctccat aattactttc
    60481 gctgcttgat gacgggacac aacgttcgtc cacccaataa aatgaaatat ttcgccagtt
    60541 tatttcattc ttaaagtgtg agctccaatg tttaaatatc tcttttgggg tattgcctcc
    60601 agaaatagct atatttatag tgcttttctt tgcgtcaagc tttttgtcca gcttatgggc
    60661 aatgtacgag gcaatttcaa atgtattgcg aagtgttgta attgtagggc ccatgatatt
    60721 aaagttaaag ttcgcaatat aaaccatcgt ctgaaaggtt cttacatgga tatcgccagg
    60781 tgttattgtt ccccatcagt tcgtctgcga cttttgggcc ccaggtgccg gccggatagc
    60841 catatagctt gatgtcgcaa ttggtttccc aggctttcaa gattgggtca acaaattccc
    60901 atgctgcttc aacagcatcg cctcgcgaga ataacgaagc atcgccaatc atgcagtcgt
    60961 ggataagcct ttcgtacgag cccagaacaa atgagttgtc gctactggca tatttaaact
    61021 caaggttggc ttgctttaca ttaaagccgg ttcctggttc tttcatgcca aacttcatca
    61081 gaatgccttc gtctggctgg atacggatga tgagcatatt tccggcctgc gttttttcta
    61141 tctctttaag cagatgagga tgaggcgtag gcttgaaatg gattacaatt tctgtaactc
    61201 ttgtcgggag ctttttacct gttcgtatgt agaatggaac accgccccag cgccaattgt
    61261 ctatgaaaaa tttaatggca acatacgttt cggtacgcga ttgggggttt acatctggtt
    61321 cttctttgta tccgggaaca ctaacacctt ttattaccga agctgtatat tggccacgaa
    61381 tgacgttttt cattatatct ttttcagtca taggccgcaa agactgaaaa actttcagaa
    61441 attcgttacg aatggcagta ggggtaatga ctgtaggtgg ttccatagca gtgagtgcca
    61501 ctaattgcaa gaggtggttt tgcagcatgt cgcgcaaagc accgcttttg tcgtaataac
    61561 caccacggcc ttcgacgcca acactttcag cagaagttat ttccaccctg tcaatatagt
    61621 tgcggttcca caggggttca aaaaagccat tgaaaaacct tgttaccagt atgttttgag
    61681 ctgtttcttt gcctaaatag tggtctattc ggtatagctg gtcttcgctg aaattgtcca
    61741 gaagcttttt gttcagcgct tttgctgttt cgaggttata gccaaatggt ttttcaacta
    61801 tcagtcgttt ccagccatct gtgccgcaat agccacacat gccagctttg ttgaggttgt
    61861 cggctattat accatataag gcaggcggtg tggacaggta gtaaatgtag ttgccttttg
    61921 tgccgtacat ttcatctaaa gaggcaaggc ggtttttgag caacatgaaa ctttcaaatt
    61981 gttgaggatc ggccgagaga aaataaatat tttcaacaaa atcagctatt ttatcgtccc
    62041 gggtaatttc tttccggcca aattgcctga tcccttcttt tattttattg cgaaaagatg
    62101 tgtcgtcaag ggctgatcgt cccatgccca gtactgcaaa gcgttgcggg agcatttcct
    62161 ggtgccggag ttcgtataag gctggcatca acttgcgcca ggtaaggtcg ccggatgcgc
    62221 caaagatgac tagtatatgg ttttcgggca tagcggtgtt atttttgttt tacttcatgg
    62281 cctccaaatt gattgcgcat agcagctacc agtttcatgg caaacgagtc gtcctggcgg
    62341 gaagcaaagc gggtaaaaag cgaagcagta atgacatgtg caggcacgga aagctcaact
    62401 gctgcatcta ctgtccaacg ggcttcgcca ctatcagcta cataaggttt cagcttatca
    62461 agcctggggt cttcatttag ggcattgcca atgagttcga gcagccagct acggaccacg
    62521 ctgccatgca tccagaggtc tgctaccttg ttgagctcag ttccgaaacc agatttttcg
    62581 agtatttcaa aaccttcggc ataggcttgc atcatgccat attcaatacc gttgtgtatc
    62641 atcttggtaa agtggccgct gccaatggct ccacagtata aatgcccccc aggttcagtc
    62701 aaagcatcga atattggggt taatgagttg caaacttctt tgctgcctcc atacattaga
    62761 caatatccgt tttgtaaccc ccaaacaccg ccgcttgtac ctgagtcaac atattcaatg
    62821 cctttttctt tgagccatgc agccctgcgt tgtgtatctt tccaattgga atttccaccg
    62881 tcaatgataa ctgcgccttt tccaacaagc gtgctcaata gttcaaggtt gtcgtccact
    62941 ggtttgcccg atggaaccat aagccatacg attttttttt cgggcagggc ctggatcatg
    63001 gaaggtatat cttttgcacc tgtggcgcct ttactttcaa ctaattttac tgcgtctgcg
    63061 tttggatccc aggctactac ttcctgtcct ttgattaata agcgctgtac catgttggcg
    63121 cccattttcc ctaatcctat aaatcctatt ttcattagct tgatttttta tttttattta
    63181 taaacaatac aatcattaaa aatgttgaat ctttaatggt tgcagaaaaa gaaaagatag
    63241 taaaaagtcg gatgttacgc aatagaaaaa tggcaatatt tatggtacta ttaatacaaa
    63301 agcgttttca cagctgtcta aaataattta aaatcaaaga tacaataatt gaatttcaat
    63361 actttaagtg ttagtaagaa caattttcaa aatgaaaact attttgagaa cagaaatagt
    63421 tgtattactt ctttcttttt tagccggata taaatttttt tagttatttt tatatacata
    63481 tcgtgttaac gcattttgtt tattatttca ctttttcatg tttcaaaaat tgaataagat
    63541 agttaggcaa ggtttgggca caaaatatat cttgatgtta agaatgggtt ggataaagaa
    63601 gtgtattagt ttcaatgaac aactcacggt tgacaagcgg attgcaaatc aggtagtggc
    63661 tatacttgct gttactgctt tattttctgg aattttaaat ataatgatgg gcttcccgtg
    63721 gtggtttacg gtgttaatat tgggggctat tgttttatcg gtactttata tcgccctgtc
    63781 tgtcattaaa actaaatttg taattcccaa agcggctctt attgttgcat tatatgttgt
    63841 agttatattg ttgtggctat ataatcctgg tttaagcaag gctccggtga ttttatcttt
    63901 tgctattatt gtttttagta ttgctattta tgttaaaaat tttgttcttc tttttctttt
    63961 aaactttata atcgttacat cattagttgt tataggagtt ttgttcccgc cattattttc
    64021 tttttcttac aaaaatgaga tacatcgggt tgtagatgtt ttaggctctt attttttgct
    64081 tgggatgtct gtagttttaa tccttaggtc aattataaac agctacataa aggcaaaaaa
    64141 tgattcggaa caacaaaaat gccagcttga ggcagctaat acccaactgc aaatcttgaa
    64201 tgaaaagttg agggctatca ataatgagct tgtcgatgca agggcaaagt tattggaaaa
    64261 tgaagaaaag ttgcagacca tttttaataa cctgcattta cttatatgta ttgttgattt
    64321 gaaaacttcg ggctttgtta atataaatcc tgagtttacc gacattctgg gttattcgca
    64381 tgaagaatta ctcaacaggc ctttctatga ttttatacat cccgatgaca ggcagaaaac
    64441 ggcttttgta atagaagacg tactgcttaa ggggaaaatg gtaatttctt ttaccaatcg
    64501 ttatcaatct aagggggggg cttatcgctg gtttaactgg aatgcattcc ctgcgctgga
    64561 aaaaggaatt gcttatgcag tggcaattga cattactgat ttgatgaaaa ccgagcaagc
    64621 ccttaaagaa agcaatgaac tgctgacgtt gtttatcaaa aattcgccca tatatgcttt
    64681 tattaaagat gtcaagaaaa agaaagccgg gttatttttg ccagtgacaa ttttattgac
    64741 atgattggtg tcaatggggc tgatatggtg ggtaaaacta tggaagaatt gtttcccttt
    64801 gagtttgccc gcaaaatatc tatggacgac tggatggtgg tacagtcaaa tcaggttatc
    64861 aacgtggatg aaaatctgaa tgaccggaat tatactactt tcaaatatcc tattttgtta
    64921 ggagaaagaa acctgcttgc cggctatacc attgaaataa ccgaaaggat taaagccgag
    64981 catttaatta atgaacagaa tgcagaactt aaaaaactaa atgccgataa agaccgcttt
    65041 atttctatcc ttgctcatga ccttaaaaac cctttcaatg cacttctcgg attgtcagat
    65101 cttattttga aaaatataga tacttataat attgatagaa ccaagtcttt gattgtttat
    65161 attaaccaaa ttgcacagaa tacctatgat ttacttaatg acctgctggc ctggtctaaa
    65221 aatcaggcag gcatgttgcc ttttatgccc gaaaagattt tgcttgcaga tatttgcaac
    65281 gaaattattg ataattacaa aatatcattt gaaacaaaag agattgtagc agaatatgcc
    65341 ggagattgcg gcatttacgt ttatgcagat gtttttatgc tcaaaactat tgtccgcaac
    65401 ttacttgcta atgctattaa gtttaccaat aaaggggggc atattaacat aaagactgaa
    65461 ataaaagata aagaagcctt aattactatt tcagataacg gtataggcat aagtagtgaa
    65521 aatatggcaa agctttgggg gcaatcttgc cagttttcta cggtcgggac cgccaaagaa
    65581 gaaggtactg gatttgggct gcttttatgt aaagattttg ttgaaaagca cggtggtaaa
    65641 atcatcgtca aaagtgaagt tggcaaaggg agcgattttc agttcaacat ccctttatat
    65701 attgaatgat aaaattctaa ggtgagaatt tcataaatat gcgaaatatt cctattttcg
    65761 cttaaacgat aaactaaaca agcatgatgg aagataattc acaaattata gaaacggttg
    65821 ttgccgggga gaaaaaactg gagttgaacg aagaagcctt gaacgagctc acccaaattc
    65881 gcaaatgggc tatgtttttc tctgttttgg gctttatatt tattgcattg atggtatttg
    65941 ccggattaat catggctgcc ttgtcgtcag gcttagcagg tgcaggtttg ggcaaattgc
    66001 caatggtatt tgttgggatt ttatatcttg cattaggtat tatctacatt cttccggtta
    66061 tctatttata tagattctcg gtagatgcca aaattgcaat tgaaagtaaa gatgctatac
    66121 ttattgcctc agcgctgaaa agcctgaaaa aacatttcag gtttattgga gtgtttacta
    66181 ttgttatttt tagtatatat gttcttgcta ttatcgggtt tgctattggt cgtatgattg
    66241 gttgaaccga aaatattggt tatatctcat gcctgttgag taaagaagcc tttatcaaca
    66301 ggcattttgt tgtattgctg agcttaatgt ttacctcccc aaaaaattgt atctttgctc
    66361 gatattttac tgcaacaaat tgaaagggat acgaagatat aaattattga ttgtgagctt
    66421 ttttgctgtg gtgctgttgt tttttgttca gcaatatact gcatcagcga ctatcgcaga
    66481 aaattataac aaccctgtta catatacatt aaagagtgcc ccgattttca tcgcttctgg
    66541 caacagcttt tcctcgccga cagaaagagt ggggcatcac ctgatggcat tgaagaagat
    66601 gcctttgttt aacctttgtt ttaagcctgt tgatgttttt tcaaatttaa taacacgttt
    66661 catttttctt caaaagtcat cattagcctc agttccttca aaactggagc tgcattgtat
    66721 cttacgtatt tgagattttc caaactattt tcattgtaaa gctggtcaat atttgtgttt
    66781 taatcgcaaa tattagtagc tcttttcccc atgaatatta ttcttggtaa aagcaactta
    66841 tcaaatcttg gttctatcct gtttaatcgt gataggtttt ggaattgaat aggttattgt
    66901 aaaatttttt aaaaagtgaa attatggaaa caaatctcaa taataatgtt gaaaatacta
    66961 atgaaaatga aaaaccaaaa ggcttttggg caagtggctg ggctttttta gtgttttttg
    67021 ccggccttat tgtcttggtg gttgcaatct cgtatcttat caagtggttt atataacatc
    67081 gaaaagtccc gccaagtgcg ggatttttta tttaactttt aatttgacat tttgttgtat
    67141 ttgcttcact cagagaaaag tgttaacttg agagaaaaat tcatgatgaa ggtcttttgg
    67201 atattttttt tcacttttgt ttttgtttta agcattttcg cccaggaaaa aaagaccttg
    67261 gaagctttaa aaatatctaa cccattaacc attgatggca ttttagatga acctttttac
    67321 tcgcaaatag ctccggcaaa ggattttgtc cagctccagc catacaacgg gaaaccttcc
    67381 tttcaaccta ctgaggtata tattttttac gaccaggatg ccgtgtatgt tggggcaatg
    67441 ctttatgaca gcagtcccga cagcatcttt aattaccttt cggaacgcga taatattgga
    67501 atgtctgatt attttggcat ttaccttgac ccatacaacc agggacaggt agcttatggt
    67561 ttttttatta cccctgctgg cgtacaaact gatttgaaag ctgttaaaag tgattatgat
    67621 tatgaagaca gcaactggaa tgctgtttgg gaaagcatga cccgaatgac agataaaggt
    67681 tgggccctgg aattgcggat accttattca gcccttcgtt tctcgggctc tgtcgaaaat
    67741 gtttggggcc tgaatatgtt ccgaaatatc aggcgttata gctcaaacaa ctcgtggaat
    67801 tttgttgacc gtaatgtaaa tggctttatc catcaacaag gacaacttat tggaattaac
    67861 gatattaaac ctcctgtaag attatcgtta agcccttatg tggcatcgta ttttgaacga
    67921 aagaaagatg gaaaggttga ttttctttac aaagggggaa tggatttgaa atatggaatt
    67981 agcgaaagtg ttaccttaga tatgatgctt atacctgatt ttggccaggt acaatcggat
    68041 gacaagaagc ttaacctttc tccgtatgaa atgtattatg acgaaaaacg ccagtttttt
    68101 accgaaggta ccgaaatgtt tcaaaaggga agcgttttct attcaaggcg cattggggca
    68161 agcccaaagt ttaatgctga agatatgaag gaatctaatg aggtggtaga ttttaaacct
    68221 aatgaaacac aattatataa tgcaaccaag atctcgggca ggtcaaaaaa tggatttggc
    68281 ataggcttcc ttaatgccgt ttcggttgaa tcgcgtgcta caatgaaaga tactatttct
    68341 ggagaaaccc gcgatgttgt ggtgcaacct gttacaaatt acaatgttac tgtcatagac
    68401 caatctctca aaaataactc atatataagc cttatcaata ctaatgttaa gatggcggat
    68461 aatccgtttt atgccaatgt tacggctaca gaatttaata tcagggataa atccaagact
    68521 ttttccctta aaggaaaagg tggccttagt gtcagggata atagctctaa agaagatggc
    68581 ttctttacca acctcggatt ggaaaaaaac aaaggaaagt ggcaatatgc agttacccag
    68641 tatatctatt ctgacaaata taaccctaat gatttagggt acctgcaaaa aaataaccag
    68701 ataattagtg aagcctgggt gtattttcag aatatagagc cttttgggat tgttcgtgaa
    68761 tacaattgga atttgtggta tgactatgtt agggttttta accctaatac tttttgttac
    68821 catcagggtg gaggctatat gaatgtaact tttaccaata actattcttt taattttaat
    68881 agcgctttgg tttccgaaca gtttaattac gatgagcccc gcgtgaaagg ccgtttttat
    68941 catacccccc cttattatta ctacaatttt aacttaaata cagatgaccg aaaacttctg
    69001 agtttttatt ttcactacgg gcatactgat gagccctttg accaatattt tgcagatttt
    69061 ggcgattttc aggctaatat gcgtatcggg caacgggcaa gtgtttatta tgggcttggg
    69121 gttaaccttg ttgccaatgt tcacggttat gttgaccatt atgccaatga cgactctatt
    69181 gtatttgcca ggcgtgatgt aaataccatt accaatgtgt tgtcttgctc gtatgtattg
    69241 aacaataaaa tgagcgtaaa ctgtagggcc cgtcattatt ggtccggggc taaaaataaa
    69301 atgttttatt tgttgatgga tgatggaagt ttggctttta atccttccta tacaaaaaac
    69361 aaagacaaaa attacaacac gtttactgtt gatatgaact tccggtggat atttgccccg
    69421 ggctcagagc tggtgtttgc ctggaaatca tcatctgatg tggaaaacga aatcgtacgt
    69481 atggactatt tcgataattt aggcaatttg tggaatgacc aggctaacag tctttcactg
    69541 aaagtgctgt attatattga ttataatagc ctgaaaagaa aaaacaagta atcaaataaa
    69601 gcttaaattt taattgtgtc atctatcttt atacatctct tgaaaagaga tgtctttccc
    69661 ctcctttaca aggaagggaa ggggagttcc tctgccttgt cacttcaaaa gctataatag
    69721 cagccgaaac cgaaacgttg agcgagtcta tccggccatg catggggatg ataatgtttt
    69781 ggtcggcctt ggtaagccaa aattggctta atccataagc ttctgaccca acaataatgg
    69841 ctgtgggttt tttataatca aattcagtat atggctttga agagtcaacc aaagctgcag
    69901 cataagattt tatgcctttt ttctttagcc actggtatgc ttgttcgttg gtacaagcaa
    69961 ccacctggtt tgtaaaaagt gtacctacac ttgaccttac agcgttgggg ttgaaaatat
    70021 cggtctttgg gtcgcaaaca ataacggcat ctacaccggc tgcatcggcc gtacgtaata
    70081 ttgctccaag gtttccgggt ttctctactt gttcggcaat aaggataaaa ggatttgcag
    70141 aaagtttaat atcgtcgaga taaagttctt tcaggtgaaa aatcgcaagc aaaccgttag
    70201 aatcttcacg ataagctatt ttagcaaaaa cttcgttggt tatttcaatg atttcagtgt
    70261 ttttagagat atgtagcgat tcaatatcag ctcctttatt aggattaaag aataatgttt
    70321 ttaaaatata gccttcttca agggcaagcg atatttcttt caggccttca acaataaata
    70381 ccttttgttt tgcccgctca gcatgttttt gcaaacggac aacatctttt attctctggt
    70441 tctgaaggct ggtaattttt tccattagtt tatttgctta ctatatcatt aaactcagta
    70501 aacttagcaa tgatagtgtt gccaacttta cttaattcta atatatcttt atctccggta
    70561 atcaagaaat cggcttttga atcaatggcc aaagagataa ggtaattgtc tttagggtct
    70621 cggcaaagat ccgttttggt attgatttca atgcattctg ctgattcgtc aagcaactca
    70681 aaaaattcct ctatttggtc tttcttgaaa aaccttctaa gttttgtttt tttgaaaact
    70741 tcagacaatt ctattaattg ttctatgcaa gtaatgatac gaatgttttg attttcgata
    70801 tggttttgaa gcccattcag gtttttgcca atgaggaatg aaatccagat gttggtatcg
    70861 atgataattt taaaggattt gtttgccttt ttcatacctg ctttttctca cagattccac
    70921 ttcttttgta atatcgtcta atgttaattc agaagttttt gttgatttca aaagcttgtt
    70981 aaaccttttt aagaataagg atttttttaa ttcattaaaa atctccaatt tgtctttttc
    71041 atccaaacga ttgatgatct taatgaattg ggttttatct aattcgatat tgactgtcct
    71101 catttttaat aaattatgat tcaaaagtaa tacaatattt actatttatt gatatctgga
    71161 cataaaaaaa cctaacaggt tttcacacct gttaggtctg tattacttta taattaagcg
    71221 ctagctacgt tagcgtcttt gttaatcttt ttgattaagc cttgcagcac tttgcctggg
    71281 ccaacttcaa caaaatcggt agctccgtca gctatcattt gttgaacagt ttgtgtccat
    71341 tttacagggg ctgtaagctg ggctactaag tttttcttga tttcagatgg gtctgaaacg
    71401 gctgatgctg ttacattttg ataaacaggg caaataggtt ttgcaaatgg tgtggcttca
    71461 atagctgcag ccaattcctg acgtgcaggt tccatcaatg gagaatggaa tgcaccgcca
    71521 acaggaagtt tcaaagcacg ctttgctcct ttggctgtca gaatctcaat agctttatca
    71581 atacctgcca ctgaacccga aataacaagc tgcccagggc tattgtagtt ggcagctact
    71641 actacatcgc tgattgattt gcaaacttct tctactacag catcttcaag gcctaagata
    71701 gctgccatag ttgaaggttc agcttcgcaa gctttttgca ttgccattgc tctttttgat
    71761 accagtacta agccatcttc aaatgaaagt gttttgtttg ctactaaagc agagaactcg
    71821 ccaagggagt ggcctgcaac catatctggt tggaagcttt cgcccaatgt tttggctaat
    71881 attacagaat gtaagaaaat agcaggttgt gtaactttgg tttgtttcaa ttcttcgtct
    71941 gttcctgaaa acatgatgtc tgtaatacgg aaaccaagaa tagaattggc tttttcaaat
    72001 aattctttgg ctaaggccga attgtcgtac aaatctttgc ccattccaat gaattgggca
    72061 ccttgaccgg gaaatacata agcttttttc atattattat ttaattttaa tagtctgact
    72121 ggcaggttag ttgccagcaa atgaatttta aagctgcaaa tgtaagtgct ttcgttttat
    72181 tgacgaaaaa taaaaagcta tttttctctg gctttgtttg taaaatattg attataaata
    72241 tttagcgcta atacaacatg ccaaacaacc atagcggaat cttgttgtaa gctccaattt
    72301 ctatgtcttc cgaaacgata aacgcatttt ctaaatcttt tatttgccga ttatcttttg
    72361 tttttccacc aacttcaaat gtccatttat catctaccag ataatcgcct ttgacaggca
    72421 gttttatggt atgggcattt ttaagctgat taaggaaaaa ggtttccctg agtgttcctt
    72481 tgtctggctg cgtctcgctg agcaaccatg atatgttggt gttctcaagg tatattttat
    72541 ctggcttttg taatatacct ggtgtaactc ctgcattgaa aagcgtttgt attatctgcg
    72601 ctttctcaag ataatgcagg tagttgataa gggtgttgcg gtgtatccca atcttttcgc
    72661 tcaactttac aatgtttggc ttgaaaggta cgctcatggc caatacataa agcagttgct
    72721 ttattttcgg tatgtttttc gcgtcaaaac catcgataaa gttcaggtct gtttctaaag
    72781 tgaggtttag cgactgttcc aaacgaagat agtacacgtt tttgttctct atgaagtaag
    72841 gatagtaacc cgttttcagg tattcgtccc agaaagccaa tggttttatt ttctggcaaa
    72901 tgtctatggc tatttcagta tgcctggcca ggatttcttt aaactgaatt ggttcaaact
    72961 ccatgttggt attgagtatg agatattctc tgaatgaaag gccttgcagt ttataaacta
    73021 cagccctgcg gctcaggtca acgtcttgtt ttaagatgtc gatgatggac gaacctgtaa
    73081 aaacgatttt catgtctggg aaaaggtcgt atatgttctt tatttccctt gcccatcctg
    73141 tatatttgtg tacttcgtct atgtaaacgg ctttgccacc ccgcgaacgg aaggtttcaa
    73201 caaagtctat caacgtgtgg cttgaaaagt ataaatcgtc gagggtgatg tatatagctt
    73261 cgtctgaaag tccgtatttt tcttttaagt gttgaatgac cagcgtggtt ttgcctgttc
    73321 ctcttgcccc tacaacgcca ataagccgcc agtcccattc taccctggtc atcaggtagc
    73381 gggtcaatgg tggttttgtg gtgctaacca gccaatctga acgccttttt aacgattcca
    73441 tttgccctat tttgaactac aaatgtaata ttgtttaatt taataagcaa ttaatttata
    73501 taaaatataa tataaacagc aaaataatat aaaaatgctg aaataaaaca gcaaaatgta
    73561 attttttaac ataaaatatt gaatgataag tttttaaaag ttaataaatg aaaatgtttt
    73621 aaaacggagg ctcataatct tcctcaaaaa tagcttcgct ggtatattca agatgcttca
    73681 aaaccatgcg caactggcca tacgaaacgg catctccaag tttttctttt gcaggtgtga
    73741 gttcctttcg acgagcatgt ttaaagtaag tgatgataag gtttagaata ggttgttcta
    73801 ctaaagcttc aatttttaaa gtaccattgc caacaaaatg tgccagatgc ccttctatgg
    73861 tagttatagc catggagcgt tctttggcaa tatcttcaat gctttttcct gattggaaca
    73921 tctccagggt caactttttt gagtctggct tcttgatgct ttgcagctgt ttgtcaaatg
    73981 aatcgggagt tgtttttata ctaatgtcct gttctttggc aaaagcataa attatatcta
    74041 atatttctcc tccaaaagcg gctattttgt tgatggttaa tccgtcaatg tcttttaaat
    74101 cttttgcaca taatggaagt ttattagcaa tgtccgctaa tatcttagtt ttaagaattg
    74161 atgtttcagc tcttcctgat tcttctgctt tttcagttct ccattttttc aaacggccaa
    74221 acaatttgct atgtttgata aagccattgc ccgaatcttc taaaactttt ttacttccaa
    74281 attcaacatc aagcatgatg cgggctttgt tatgcaggta ttttgaaata ctgaaattgt
    74341 ttgctgttaa ttccaagagt ttccgctttt gtataacttt ttggcaaagc ttttcaaatg
    74401 agcttttaag ggagttacgt acagctttat tgtcagtttc tatctggata tttttgcagt
    74461 gttcccatat ttgttggtct atgttgtctt tgaaatactg acagcctttt ttgatgcggt
    74521 cttgaatggt ttcgctcgtt tctaaatcct ctgtatccgc agataattgc tgcaattgca
    74581 ccacaaactt atcgcaaaca ggggtaaatt ggtcgttgat actttttagc atttgcgaaa
    74641 aggtaaggtt gaggtttccg gcaaggatgt tggcattctc tctcgatatc ttgatgcatg
    74701 ggtaaagaag ctttgctatc tctttgaagt caaacaaatc ttgtagtaga tggatttgat
    74761 attttttttt tgcttgtata aagctgtcta tagttggaga taggcttggt attttcctat
    74821 tgtactgtga tatatggtat tcggtgacta aagaacctct gtttacaggc gaactaagta
    74881 ctatcccttc taaggtacgg caacgactga gggctacata tacttgtccg tgcgaaaagg
    74941 catcctgggc gtcaataata acgttgtcaa atgttagccc ctggcttttg tgtatggtaa
    75001 tagcccaggc cagtcgcaac ggaaattgtg tgaaaagccc tatttcttct tcataaattt
    75061 ctttggtctc ttgattgata gagtatttaa agttttgcca tttttgacgg tttactttta
    75121 ttggttcttg atcatttggg cactgaactt caataatatc ctcgtctata gaaatgacaa
    75181 tgcctatttt gccattaaaa taacgtttct caaaagatat atcattcttt atgaacatta
    75241 cttgtgcgcc aactttcaag gtaagctcaa aatctgcagg gtaggcataa tctggaaatt
    75301 ctccttctac ttttgcagtg aatatgtgtg gttcgagctt aatttggttg agctttttcc
    75361 agtttaacgt gttggctttt tcgttgtggg tggtcagagt gatataacca tcgttgtcat
    75421 caggctcaaa tcctctgatg tagcgcgtgg atagatggtc aagcgcttct ttgtcaaggt
    75481 tgttgtcccg tattttgttg aggatgttga taaacacttc gtcagattga cggtagatgt
    75541 gctgtaactc aatgcataca tagtctgttt gttgcaaagc caagctgtca aaaaagaaag
    75601 gcgaattgta ataaggacgc agcaaatccc attcatcgcg ttttactacg ggcggtaatt
    75661 gctgcaaatc tccaatcagg agcaattgta cattgccaaa aggttcgtcg ctatgtctta
    75721 tctggcgtaa ggcctggtca atggcatcca gcagatcggc acgtaccatg cttatctcgt
    75781 caataaccag caaatctaag cttcggatga tggataattt ttcctggcta aatttaaagg
    75841 agcgttcgtt gctaccagtg ataaaaccat ttgtaatatc ttgtcttgct ggtgtccatg
    75901 gcccaaaagg cagttgaaaa aaggaatgta ttgttactcc gcctgcattg atagctgcaa
    75961 cacctgttgg ggctactact accatccttt tgggcgattc gttgcgcaag tgtcgcagaa
    76021 aggttgtctt tcctgttcct gcctttccgg tcaaaaatac atttttcttg gtggacgata
    76081 agtatttgaa agccaggtct atttgggggt tgtccgaaga tagcatgtgc tgcattttaa
    76141 tgtgtattat ttcgaaatca agttttctaa atcatcgata tctttatgtc tgccagatgc
    76201 ttttttgtta agaataaggt ctgaagtaga aatgaaaggg attttaatgt tatcaatgtt
    76261 gaaatattct tttctggtat agcattcaga gaacttgaca ccatcaatgt cgtttaaaat
    76321 atcaatccta agcggaggat aaccaagttg tactacattt ccatctttca ggaaatcgtc
    76381 agacgtgagg tttaatgagg agaatccaaa gtcttttaaa acttcaatta taagtattga
    76441 attttcttgg gttttattaa tccagatgtc caaatcgccg gtatatcttg ggtagccatg
    76501 tatagttacg gcatatcctc ctactacaag atattcaact tttttttcgt tcaataattg
    76561 tataaactct ttgaaatctt ggctgaacat ggtactttaa gtttatatag ttttggcgca
    76621 gggtttcaag tgcgtctatt ctttcttgcg gtgatttgct tgcccaaaaa agaaaatcat
    76681 ttgtcttttg atgaatattg atgattttta caattttctc cattgctata aggcttatat
    76741 gatgtaaaga tacagattat tcaatagaat aaaaacttat ttatttgcct acctttgcgc
    76801 aaaaatcaaa atatggcatc ttacctgcaa atagagaatc tttcaaaaag ttatggagag
    76861 tttgtaatgt ttgagaattt ctcctttcaa ctttctaaag ggcagaaagt tgctattgtt
    76921 gctcgtaatg gagctggcaa aaccacgctt tttaatattc tttgtggtga tgacatttca
    76981 gacggcgggt ctgttatctt tcataaagat attaccgttg gatttctcag gcaggacccg
    77041 ccattcaatc cttcactcac ggtgttagaa caggtacttg attcttcagg gaaagttaaa
    77101 gatgctgtcc agaaatacgg gcatgccatg gcttcaggta atgaagagct tatgcataag
    77161 gcggttgaac aaatggatgc tttgcaggca tgggattttg aggcaaaaat gaaacgcatc
    77221 ctaggccagc taaaaatatt caaccttgag cagaaaatga accagctatc tggaggacag
    77281 cgcaaacgtg tagctttggc taatgttttg ttgcaaaacc ctgatttact tatcttagat
    77341 gagcctacaa accaccttga cctggatatg attgaatggc ttgaggaata tttgcaaacc
    77401 tattgtagta cgttgctgat ggttactcac gataggtact tcctcgacag ggtgtgtaac
    77461 gagattttgg aaatagacaa taagcaagtc tttcattata aaggaaatta tagttatttt
    77521 cttgaaaaac gagctgaaag attagaaatt gaacaggtca atattgataa agcacgcaat
    77581 ttataccgaa aagagcttga atggatacgc cgaatgcctc aggcccgtgg aacaaaggca
    77641 aagtacaggg ttgatgcttt tgaagatgtc aaggaacagg cttttaagca aaaatcgaat
    77701 aaagaaatca atataaactt ccaaggctcg cgcctggggg ctaaggtgat tgatgcagaa
    77761 catgtctcga aaaagtttgg ttcgcttaat attatcaatg acttttcata caaatttgca
    77821 gcttttgaaa agattggtgt tatcgggcct aatgggactg ggaaaaccac gttgttaaac
    77881 ctccttactg gctcgttaaa accggataat ggaattgttg aacacggtga aaccttgaaa
    77941 ataggctatt acaggcagga tgggcttaac ttcaatgagc aaatgaaggt aattgacgtt
    78001 gcccaagaag ttgcagaggt ggttacaatg cgtgatggca atcagctcaa tgtctcgcaa
    78061 ctgctcaata tgtttctttt tccccctccg gtgcagcatt cgtttattta taaacttagt
    78121 gggggcgaga aacgcaggct ttatctgatg accatcttga t
//
LOCUS       DAOSPF010000007        77428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_338_length_77428_cov_6.893929, whole genome shotgun sequence.
ACCESSION   DAOSPF010000007 DAOSPF010000000
VERSION     DAOSPF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 77428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77428
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_77428_cov_6.893929"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1071
                     /locus_tag="PK252_02460"
     CDS             76..1071
                     /locus_tag="PK252_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT13600.1"
                     /translation="MKRLIFFTGVVGLTFSVFGQTKDSLKYQSDSIDIRYLYKINDTL
                     SYRYYPTRRFGFVSNAFKDFYLLPTELVKKENFKGLGIIAASTAILIYYDQELVDAAQ
                     RFGRYINLSGENNVYNLTGTKDLRINVPTDLSSGLYYIGDGITEMGVCAGFYIYSSFT
                     DDKRAAQTAAQLAEGMLAVGAWIQILKHLTGHESPQCATVSGGRWRFFPNPVDYQNNV
                     PKYDAYPSGHLATAMMTATVVSMNYPEYKFIKPLCYTLMGLCGYQMMNNGVHWAGDYP
                     LGIAIGYAVGRMAVNRGREEVKQPLTSIREPKASFQLHPVYMPEGATGLALQVKF"
     gene            1255..2007
                     /locus_tag="PK252_02465"
     CDS             1255..2007
                     /locus_tag="PK252_02465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT13601.1"
                     /translation="MITPEQTYQKFAVFYDLYVGRFSADVDFCHSYCSSDKSIVEIGC
                     GTGRILKYLLDKGLSVTGIDISDEMLQKAGAKLEPFVSSGHLKLLNHNFAEKPFETKF
                     DVALLTFYTFNYILDKPEAFLANIHQSLSRQAALLIDVFYPMPLKNKAIDNVWTEKSF
                     EIEGQQVLLKDKRTMNGNIEHRCQVFSINGSEGISIDTFRRYFPPKEMVYLLKNADFS
                     DVKLAPGYDFRSLKCELEESELGDNFIVVAKK"
     gene            2045..3121
                     /locus_tag="PK252_02470"
     CDS             2045..3121
                     /locus_tag="PK252_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13602.1"
                     /translation="MKKIVFLFSIASFLLVNIAANPLPGTDIKIKAFYFDDNDNWVLK
                     LDAYSLDYYNILDIDTVWVCSSTECARINKTFVDFNNLDIITEDSLDRPLKFNKEGDS
                     IRVKFYYKHFRNPGSDSVFIETLPDWYGASLTYGNYGNSNVPRLSKGQSIASVSYYGY
                     DYVYYSLSNTITDFNDTTKMFGVLKGHIYDKNGRPLNKGQFTIDFGRILKVQSGLYSV
                     KMYSRKYKLNSAYLPSNYNSSVHFKPIEFEILPDSTTERDIYLTDYVGVKSISNKENL
                     VKIFPNPVKGNSILQYAIALPVFSSNCQLNIININGQSVFQCKIMENKGNIQLPQDVF
                     DGTYTVQVIMNGAVLHSSQLIVVK"
     gene            3118..3675
                     /locus_tag="PK252_02475"
     CDS             3118..3675
                     /locus_tag="PK252_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 family protein"
                     /protein_id="HOT13603.1"
                     /translation="MKRGTFFILFGLFWIHLFSQNNVQFKAEYIDFKLDSTQFAINGI
                     YEFKNNSAKDVEIPIVFPFAEDVKDIDSIRLISLNNMSALPYEKIKKEIRFRLHIPAK
                     DSLSVNIYYSSRSKKKNVYILTTTRFWEKPLEIAYYSLAIDKGINLKTLSLKPDSSSV
                     KYGEQVYFWKKEKFLPEKDFVVEIE"
     gene            complement(3679..5640)
                     /locus_tag="PK252_02480"
     CDS             complement(3679..5640)
                     /locus_tag="PK252_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13604.1"
                     /translation="MKNILHKIVFTLFTLSFAGNIHAQLNINHWLNAGREQIDKDNTV
                     AIEYFSKLIKFIPDLDEAYYLRAYSKYSLKDYVGALQDINTAIKIKGGNSYYYILRGE
                     IKSRMYNVRGAREDFNKAVMLKATNTEAYLRRGLNGLMLGLNDEAIQDFNIAITLNKN
                     NDNAFLYRGMAYQAKKDYYMALCDFDKAIQLNPFDAENYFRRGRNYLEEKKYNEALAD
                     FNQTIKLDSTHTPAYYSRALTRDELKDSLGALIDLNRVLKLDPENALAYFVRAELRVR
                     FKDINGALDDYDKVIEINPNNIYTYFNRGIVYHTLKKYKKAISDYTTAINLNSNFVAA
                     YYNRSIALRAIGDMYNAEKDYKTAARINKERNESGFEEKLDSTNLAKIIDFKADFERG
                     NVKESSNSLAGITPAPAYGIVCISKTDFENKKYTNTNVLEKINNQILGEERLIVSNTA
                     IDNIYDQLNSWLAEFENLSENENNATRLLSRSVIKAQLQDYNGALSDCDLVIKLKPDW
                     AVAYFLRSYVLFEYIMLMQNVSSKESDFFFIGENGNKLPSSVKGNPDFTQVISDLETS
                     IQHDSSFIYTYFNLANVLVETKTFDKAIYYYSKVIAIDPNFAQAYFNRGLLYIFTNKK
                     EEGCSDISKAGELGIKSAYNIMGKYCNEK"
     gene            complement(5655..6017)
                     /locus_tag="PK252_02485"
     CDS             complement(5655..6017)
                     /locus_tag="PK252_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor"
                     /protein_id="HOT13605.1"
                     /translation="MPKDNAFFSGKILPSCLLSVIFFMSKNNNKVLKFLKNKYFLASL
                     FFVLWLIFFDQNNLIDRVAALKKYYEMKEEKEFYIEKIKIDSHKLNELRTDRDNLEKF
                     AREQYFMKRDNEDIFVLE"
     gene            6038..6826
                     /gene="surE"
                     /locus_tag="PK252_02490"
     CDS             6038..6826
                     /gene="surE"
                     /locus_tag="PK252_02490"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT13606.1"
                     /translation="MDKDRLILITNDDGIHAKGIEALVNVAKSFGNVVVVAPTEGQSG
                     MSHAITVKTPIRVYKHGKNGNVEKFSCNGTPVDCVKMAMSKILPKEPDLILSGINHGG
                     NSSSSVLYSGTMAAALEGCLYGIPSVGFSLLEFSKDADFSAATFFVNRIIDNILRRGL
                     PKGVCLNVNIPYLKAEQIKGVMVCRQNHGIWREEFEHRTDPAGHDYYWLTGTFEDLEP
                     HATDTDEWALRNQYIAITPVQFDFTAHKALHTLKQWNWNDGTTD"
     gene            6807..7145
                     /locus_tag="PK252_02495"
     CDS             6807..7145
                     /locus_tag="PK252_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13607.1"
                     /translation="MMEQLIEKIKAKVARFDKLYVGIILGLLVPVVALMGTYFYTFTN
                     YTLNQFFYFLIMLRVITKLFSLCIIPNLAVFFIFLWLDMNKAAKGVLTITFIMTVLIL
                     AYQAHMGLLW"
     gene            7210..8457
                     /locus_tag="PK252_02500"
     CDS             7210..8457
                     /locus_tag="PK252_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT13608.1"
                     /translation="MVFLNLILESLQMAWHSISRNKVRTSLTLLGITIGIFAIISVFT
                     ALDALENGIRDNVASLGDDIIYVQKWPWSFDDDYKWWEYMKRPVPRVADYEEVLKKAQ
                     TVDAAVFSVSTGRTIKYKNNSFGNGGIWANTDDFEKVKAFEIEKGRYFSPYEFQSGKN
                     VCIIGNTIATQLFGNEDPVDKFIKVDGRKIKVVGVTKKEGTSIVGGSMDDVVLIPLNY
                     ARNIFDIKSDMLNPFMMIKAKKGVPLDQMKDELRSILRKKHNLKPLQPDDFSLNQASL
                     IISGIEKIFLIINIAGWIIGGFSIIVGGFGIANIMFVTVRERTNQIGIQKALGAKRFF
                     ILIEFLFEAVILSVLGGLLGLFFVFLASVAASKALDFEMTLTIGNILLGILTSAIIGL
                     LAGLIPAYTAARLNPVDAINTTF"
     gene            8457..10283
                     /locus_tag="PK252_02505"
     CDS             8457..10283
                     /locus_tag="PK252_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT13609.1"
                     /translation="MQKQTALPFFPTSAKEVEQLGWTSIDVILFTGDAYVDHPSFGVA
                     VIGRVLEKQGLKVAIVPQPNWQDDLRDFRKLGKPNLFFGITAGNMDSMINHYTAAKRL
                     RSNDDYTAGGKAGRRPDYAVKVYTCIVKKLFPDVPVILGGIEASLRRFTHYDYWSDSL
                     MPSVLAETKADLLFYGMAEKALEEFVKLLQAGKPFSEARKIPQLAYLVKDKADIEVED
                     GAKTIYLHSFEKCQKDKLAFGENFKFIEEESNKIHAARIVEPVGNGFVVVNPPFAYLT
                     QDENDAPYELPYSRLPHPRYKEAIPAFDMIKNSINIHRGCFGGCSFCTISAHQGKFIR
                     SRSPESVMREVLNLSKMPYFNGTITDLGGPSANMYMMSGIDKAICEKCKRPSCIFPAI
                     CKNLDTNHKPQNDLLQAARDVAGIKHVFIGSGIRYDLLFNAPNVETKKSSDEYFANLV
                     KYHVSGRLKVAPEHSSDEVLKLMRKPSFALFKRFKDEFDKLNARFGKRQYIIPYFISS
                     HPGSTIDAMAALAENNRQLNINPEQVQDFTPTPMTLSSCIYYTGIDPYTGKKVYVARS
                     KDDKKMQNAFFFWYKPEYRKILFEYLKRIKRTDILNKLYPRR"
     gene            10362..12620
                     /locus_tag="PK252_02510"
     CDS             10362..12620
                     /locus_tag="PK252_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT13610.1"
                     /translation="MKIKLLTIVAISCFVASLTSQTMNIRINQLGYYPQSSKVALVVW
                     PMGNEFEVLQLPDSSVVYSGNLSAKKYYNDSKDSISIADFSSVSQPGQYCLRVKGMAV
                     SLPFVISDNVFREVAYASLRSFYFQRCSYALDEKYAGIWKRKAGHPDTSCVYHASTGR
                     SGKVSSPKGWYDAGDYGKYVVNAGISVGDMLQFYELFNTYFPDSSASIPESGNGVNDL
                     LDEVRFELDWLATMQDTDGGVYHKITTAGFPGFIMPEDDKDIRYILGKCTSASLDFAA
                     MMAMAGRIYKKADSAFAANCLAQAKKAWDWAVLHPSVAFTNPVGISTGGYGDNNFSDE
                     FLWAAAELLISTGDSVYYKYLVSKKSALVASVSGWGSVGGLAAQSLAVMPSILDTAIV
                     SKIKTSIISVANSHKSNIQGSAARVSNTGYYWGCNGAVSQTGILLIYGYLLSGDSSLL
                     NASIELADYQLGKNATGYSFISYYGKKLVMNQHHRPSIADGVVMPEPGFISGGPSAGK
                     EDNKNMYAYTTNAQSFIDNTSSYASNEVAINWNASLTAHLAAIDAILGNKDAFVMPVV
                     SSVVNPANLTLTKPAHNASFYVGDSIEVKVTANAAEIQKIEFYLNNKYLKTVYDAASP
                     FKLVLPVGTYTVTACLIDKNGLVTEKAALITVKPKTGVDAIKNESRLKVFPNPGSKKI
                     TVQYNASLFGKVVLQLCDISGKKTVLWTGQMKNEKNTLDITIPASLTAGVYMLEVVEG
                     AKVIESNKVVLQ"
     gene            complement(12641..13984)
                     /locus_tag="PK252_02515"
     CDS             complement(12641..13984)
                     /locus_tag="PK252_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT13611.1"
                     /translation="MHHRISFEYNLITLFLLLFTAFTYPSISGQEKKANKNNSEVKAL
                     PPMYYEMKIELLNHNSAIKLDYNADVQKYIDLYLTQRKEKIAEFLELSQYYFPIFDRY
                     LDKYQLPFELKYLAILESNLDPAARSSSGALGLWQLLADASKLLGLEVSSYVDQRCDV
                     YASTDAACRYLKYLYSVFNDWQLALAAYNGGPGEIKKAIERSGGKTTFWEIQPYLPEQ
                     TKWYVPAFIAIVYLYNQAADHGIYAAKTGFGSISTDTLQIMQALEFDKLSEKIGMSID
                     SIRKYNPCYRRDYIPMREKGLTLVLPSDKVLAFLKRENQIYSQTNDTLDMFQKMEASS
                     NTQGMVKTFYEVKKGDFLHKIALANGCTADNIMIWNKLKSQNLKVGQQLIIWKKDNTK
                     NKKDNYFEYTVKKGDTIWGIAQRYKCDSINDIMLINNLHSEQSLIPGSIIRIRIR"
     gene            complement(14004..14195)
                     /gene="tatA"
                     /locus_tag="PK252_02520"
     CDS             complement(14004..14195)
                     /gene="tatA"
                     /locus_tag="PK252_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT13612.1"
                     /translation="MENLHVMLGAIGTTEIILILAVVLVIFGGRKIPELMRGLGQGMH
                     EFKKASKGETNNTPDEKKQ"
     gene            complement(14206..15528)
                     /gene="rseP"
                     /locus_tag="PK252_02525"
     CDS             complement(14206..15528)
                     /gene="rseP"
                     /locus_tag="PK252_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT13613.1"
                     /translation="MEILIKALQFLLSLSLLVLLHEFGHFFFAKLFKTRVEKFYLFFD
                     PWFSIFKYKKGETEYGIGWLPLGGYVKISGMIDESLDLEQMKQPAQPYEFRSKPTWQR
                     LLIMLGGVTVNFLLALAIYVGILFTWGEEYLPTKNVTYGIAVDSVAYNAGFRNGDKIL
                     TVDYKEVENFFKIVPTIVLEEAKTVQVIRDGKLINIALPENLNYTLIRKKDFFTVRIP
                     YIVGDVVKNTAAEKAGLKPGDKVVAVNDSQVIFVDQIKPIVQANKNKNITLTIEQNGV
                     LKKIDITVPETGLLGVVLDGNISKYFQLKHNDYGFFASIPAGISMGFNTLSNYVKQFK
                     LIFSKETKGYESLGGFITMGNLFSGVWDWESFWALTALLSIVLAFMNILPIPALDGGH
                     VLFLLYEMVTGRKPSDKFLEYAQITGIVILFSLIIYANLNDIIRLFTH"
     gene            complement(15552..16706)
                     /locus_tag="PK252_02530"
     CDS             complement(15552..16706)
                     /locus_tag="PK252_02530"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT13614.1"
                     /translation="MKRKKRIAILGSTGSIGTQALDVISNNLNSFEVETLTANNNAEL
                     LIKQAMQFTPNQVVIANEEKYQFVKEALANYPIKVFAGEKAIAEVVQFSSIDIVLTAM
                     VGYSGLTPTINAINAGKTIALANKETLVVAGEYISKLAIEKRVNILPVDSEHSAIFQC
                     LTGEFHNPVEKILLTASGGPFRGKDINFLKNVTPEQALNHPNWCMGNKVTIDSASLMN
                     KGLEAIEARWLFGLQPSQIEVVVHPQSIIHSMVQFEDGSIKAQMGLPDMRLPIQYALS
                     YPERLKASWPRMNFADYPALTFEKPDLTVFRNLAIALDAMDKGGNMPCAMNAANEIAV
                     EAFLCGHIGFLGMSDLIEKTLSKITFIKDSGIEDYQQTDKFARELALQLI"
     gene            complement(16722..17591)
                     /locus_tag="PK252_02535"
     CDS             complement(16722..17591)
                     /locus_tag="PK252_02535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT13615.1"
                     /translation="MQKSERKLSDRLRDKYRLIIYNDNTFEEIGFVRLKGFNLLWIIG
                     ALGFLLISLTFILIAYTSIRELIPGYPDNTIKREMVMNALKLDSLEHELEIRDRYLAV
                     ANDILKGNNPVSYGEMTKDSVKIKEIIFTKSPKDSLLRKKVEEEERFNLTPPNQASNN
                     NTTQTLAQVHFFSPLRGIISNGFNPNINHLGVDVVSEPNAVIKAALDGTVIMATWTLE
                     TGYVIQIQHDNNIITVYKHNAELLKQSGNRVVAGEPIAIVGNSGEISTGPHLHFELWN
                     NGHPVNPVDYIVF"
     gene            complement(17603..18010)
                     /locus_tag="PK252_02540"
     CDS             complement(17603..18010)
                     /locus_tag="PK252_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOT13616.1"
                     /translation="MPKIFTATDIQQFDKEVQKDYFDRHTPVIDCPSEVNENERFPVR
                     IFLGSQYAHPDDGDHFIGKVQLWNRETLLAEATIFPGAMGNQPGHVEVVFTIIPKVSM
                     NLTAMSYCTKHGLWQSLPKEVKVNHPDQQCSKS"
     gene            18322..18771
                     /locus_tag="PK252_02545"
     CDS             18322..18771
                     /locus_tag="PK252_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOT13617.1"
                     /translation="MSLFDKIGEDIKAAMLAKEKEKLEALRAVKAAILLAKTEKGASD
                     ELSPDAEMKLLQRLVKQRKESAEIFAGQNRSDLADKELFEASVIEQYLPKQMSEEEIK
                     AALKIIIEKVGAKTPADMGKVMGVATKELAGKADGKTISSLVKVLLG"
     gene            18863..19201
                     /locus_tag="PK252_02550"
     CDS             18863..19201
                     /locus_tag="PK252_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13618.1"
                     /translation="MKPLKSGTALAYWLLRIALFIFLFTIFYSEIGNFNFHNLESYFA
                     VAFVALGALLFVGGFMAKPTLTVISGILITLLSSYSLFVQFKAGLNASIASFIVVFSI
                     GFYFTCVGNQ"
     gene            19206..19946
                     /locus_tag="PK252_02555"
     CDS             19206..19946
                     /locus_tag="PK252_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13619.1"
                     /translation="MIKKALYISFTFLLSGNVACNLYAQKTNELQEATVVYKKKPAAQ
                     SAAETSTSATSGNTAPAEPPKPVVKLDTIVRLGGKKIICNVKKVNPTTVEYVKPGNPT
                     LVEIKRKDIEKIVYSNGRKEVYNKPIFSVLDKLQWQAVLVTENEAEVSGLYKVGVVKA
                     NASSSSRSPKAAKQSAIIRLQKKTANMGALIVLIINSEMKGGYGEIPGWEIEGIAYSD
                     TPPVDTASVNQQIRKLIEKNKQRVNASK"
     gene            20007..20924
                     /locus_tag="PK252_02560"
     CDS             20007..20924
                     /locus_tag="PK252_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT13620.1"
                     /translation="MDYNLIKDKVFSAGWFKAWSFIVVGSFILAAGFVFFITPYKIVP
                     GGVYGIAIVIHYLTKGLFAFAPEGLPIGTMGLVMNIPLTWLGIKILGPRFGIKTIIGF
                     ILSSVFIDLISYYYGYEPLVKDDALLSSIFGGVLIGFGLGLIFKSKATSGGSDIIAMI
                     LAKYTRLPLGQLLIYVDSTIVLVGLIAFMDWRIPLYSWIVIYITGRVIDGTLEGISYN
                     KTLLIISDKYEDIRTKILYDLNRGGTLIKAEGMYEGKERKLIFTNVSRREAYILKEYI
                     KQVDPDAFVTIIDANEIIGDGFKSFKESL"
     gene            complement(20931..21932)
                     /locus_tag="PK252_02565"
     CDS             complement(20931..21932)
                     /locus_tag="PK252_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT13621.1"
                     /translation="MKNYFLSFILGSLILSISCTKEKTTIGFMLPNMLNKRYEVERDL
                     FTKKINEAGGEILFYSADNDALLQEKQLDEMLQKGVNLVVLDPVNRFTAAGMVRKLHN
                     KGIKVISYDRLIANCQPDLFVSFDAHVIGEQMASYAISKKPYGNYVILSGDKSDINAV
                     WMNEGFHKVIDPYVKSGKIKLSYEAYMENWSEDDAYNTMKKYINYSISVPDVILSAND
                     NISRGCINIITQKGYSPENIIITGQGAEPLACRYMLQNKQSISIYKSVKKMAALVAEL
                     SIKIAQGEDIAKIAPTTINNGKFDIPVIFLETQVVDASNIRSVVVADSMISEKDLEY"
     gene            22113..22334
                     /locus_tag="PK252_02570"
     CDS             22113..22334
                     /locus_tag="PK252_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13622.1"
                     /translation="MKVVRYLAWASVGIAALIMVLAIISYFTKHLLLNVGHGATFLHA
                     INSFLLLGIALFIASKQCCCDKCCNKDEK"
     gene            complement(22391..23584)
                     /locus_tag="PK252_02575"
     CDS             complement(22391..23584)
                     /locus_tag="PK252_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT13623.1"
                     /translation="MSGKNMQANINRLYVIKIAHWFMLTMPVILLFYNASHLSVRHLF
                     IAQAAYSLTIVLLEIPSGYAADRWGRKNTIVIGALFGFLGFLAYSISSAFIVFVLAEI
                     MLGIGQSFISGADTAMLYDTLIAHKKENDYLKIEGRMTSLGNFAEAFAGITGGLLAAI
                     SPRLPFIGQACIALIAIPAALGLKEITLSEGKNKVTFRSIVNVFRDVVSKPSQLMIYM
                     LFSSVIGSATLSMAWFAQPFFKVIHLPSALYGVLWTLLNISVAITAFVAFRIERRWGE
                     KNTTLAIGLFTIVAYIALSLTQTYWGLIILFMFYLVRGIATPLLKDSINKRITSDIRA
                     SVLSFRNLIIRLFFSAMSPLFGWAADFYSLKTALMLAGTTLAVLIVPLGILLYSWHLK
                     YPEKK"
     gene            complement(23624..24283)
                     /gene="hxpB"
                     /locus_tag="PK252_02580"
     CDS             complement(23624..24283)
                     /gene="hxpB"
                     /locus_tag="PK252_02580"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HOT13624.1"
                     /translation="MTACVIFDMDGVIIDSEPLWRETLIEVFGEIGIKLTEDMCRQTM
                     GMTTKGAVDYWYEKLPWKGFSIEEVISKIEFSIIEKIKAKGTAMTGVVDVMQMLKKQG
                     IRMALASSSAIKVIETVLDKLDLRQYFELYHSAQLEAKGKPDPAVFLSTARMMNYHPS
                     QCLVIEDSIHGVSAAIAAGMKVVAIPDPVLFNDKGFDKADLKLKSLKDFTLECFLSIN
                     N"
     gene            24523..25479
                     /locus_tag="PK252_02585"
     CDS             24523..25479
                     /locus_tag="PK252_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOT13625.1"
                     /translation="MTNKIAKPFLKWAGGKTQLINDIERALPKDIYTNKFTYIEPFLG
                     SGAVLFWMLNNFPNLKKAVINDINDDLINTYKIIAFKPNELISILEILQNEFHGLEGQ
                     DEAKKKYYYSKRDLYNTRKEEQTGQAALFIFLNRTCFNGLYRVNRKNEFNVPMGSYKR
                     PTICDKENILAVSKALQKVEILCGDFEETLNYADKNTLFYFDPPYKPLSETSSFNSYS
                     RDEFNDAEQIRLRDFCYKLDTLGHTWILSNSDVKGKDIKDNFFDNLYSDFNIQRVYAK
                     RSINANPENFYLGVPLSGRAIRSKSSLRCGLSTTIPNATYCH"
     gene            25479..26258
                     /locus_tag="PK252_02590"
     CDS             25479..26258
                     /locus_tag="PK252_02590"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HOT13626.1"
                     /translation="MNLSFNIKLAEGYQSNSQIARVLTENWVLTNSYCPSCGDLPLSG
                     FQNNRPVADFYCKKCSEEFELKSKSGKLTNTITDGAYSTMIERISSENNPNFFFLTYS
                     NNWTVNDFLIIPKQFFTEEIIIKRQPLAPTARRTGWIGCNIDISKVAESGKVFLVKNS
                     KVIDQEVVKQSFNKTLFLRQQSSDAKGWILDIMKCVDEIKTNEFNLDDVYAFEKKLKL
                     KYPDNNFIKDKIRQQLQLLRDKGMIEFVGRGKYRKIQYGNI"
     gene            26245..26403
                     /locus_tag="PK252_02595"
     CDS             26245..26403
                     /locus_tag="PK252_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HOT13627.1"
                     /translation="METFKIEIQEFLSRIIEVEANSKDEAIAKVRELYSLEEIILDSE
                     DYVTTEIN"
     gene            26475..27281
                     /locus_tag="PK252_02600"
     CDS             26475..27281
                     /locus_tag="PK252_02600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT13628.1"
                     /translation="MVFADPPYFLSNNGLTIQNGQIVSVNKGNWDKSHGFDHLNEFNR
                     TWLSLVRDKMKDDATIWISGTMHNIFSVGQILTELGFKILNIITWEKTNPPPNFSCRY
                     FTYSTEQIIWARKSEKVPHYFNYELMKQLNGNKQMKDVWRLPAIAPWEKSCGKHPTQK
                     PLSVLTRIILASTKPNAWILDPFTGSSTTGIAANLANRRFLGIDQEEDFLAISKNRKL
                     EIENPKTAATFRQKIGGFNDKKGLELFLVEEPEEEYKTELNLTTKGSIQQ"
     gene            27702..28214
                     /locus_tag="PK252_02605"
     CDS             27702..28214
                     /locus_tag="PK252_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT13629.1"
                     /translation="MDIQRTGIDEIDFDQIFKQWYGAIRNFVYYKIGDVTKAEDIAQD
                     TFLKLWEKRDTIKTETVKSLLYTIANNLTINVIEHNNVTFKFENKYKPDTKHPSPEYD
                     MEMKEFDQRLQQVLGQLDEKQRTVFLMNRIDEMTYNEIAQSLGLTVKAIEKRMEKALS
                     YLRKNIAVKI"
     gene            28226..29104
                     /locus_tag="PK252_02610"
     CDS             28226..29104
                     /locus_tag="PK252_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOT13630.1"
                     /translation="MTPTYANNNIDEQILDTVSGYQPPFETTTDEALAILKKKIAQGA
                     KAKDVKESKTIPFKSIVLYASGIAASLLAIVATWYFFSYNARVTVLADKGQHTECVLP
                     DGSVISLNAKSAVTYRKQTFRQERFIEMEGEAFFNIKKGSVFTIKTKHADIKILGTSF
                     NVYARESMFKVACVTGRVLVANVEDTVIIEPGESASLFNRSLKKFRDEKIASETKWRN
                     GEFIYENASLKIIFEEIERQFNVNFALPDDVESKYFTGGFSNKNLVDALDIVCIPMGL
                     TYEIGANGYISVHEKY"
     gene            29121..31739
                     /locus_tag="PK252_02615"
     CDS             29121..31739
                     /locus_tag="PK252_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT13631.1"
                     /translation="MLTLLFIGTLPVDSQQYKLSLRKTNLADAMEQTSKLLNIRVAFD
                     AQKLSQIAIDKEISGNTPDEVLKKLLANSGYSFEFRHDNFLIYKSEEKSDENVCQIIG
                     SITDIETGEQLPYATIKIKDQNIYVSSSENGTFYLKQSNAKSIQLNISYIGYYPLDTL
                     VYCNAPKVVCKLALKKNIQELEKVEVREPKIEMVDYRNDVDFATTINASKLLDLPMFA
                     ETDIFRALQLLPGVSFSESSSELNIRGGSSDQNLVLLDGQTLYNQSHYFGVFSALNPN
                     VVKDVQVFKGGYDSRYGERVSGIVDITGKNGNKLKPTVYGDVNLINGNIAAEVPVSKK
                     LTVIMAGRRTYGDIYSTEFANNLFEKDSNRFSNPSQRSLSYSKPKFSFYDYNTKVSYQ
                     LNDGENVSFSVYGAKDSYDNKYNTGDKNFLASIHENNKWGNYGLNASWLKQWNGALFT
                     NVQIGSSGYSDTYNSSSSLSGTGGPHYPSGPPFQNKNYDSYSYNELNDYSASVKGIWN
                     QNGKHQFSFGATVRQNKIYYHKDADRVYIYENMNQAAVISSLFLQDRLIITDRLTIKP
                     GLRISFYDGNNGWYSEPRFAANYRLTDAWSVRFATGRFYQYINQVQVLQETGYNKNFW
                     VLTDDSIHPVVKSNHYIFGTSIDLGSFFFDVEPYYKTYSGLQEYIFVSNYTRDSTFRN
                     LFDTANMQMFAPKERPGLFIHGQGKSYGIDFHVRYKYNNFTTWLSYSLSKSTRQFSEI
                     NNGNEVPASTDQRHQITWTNMYAFKNWNFGLTNYYSTGRPYVDLSIDARRMPTIRSYK
                     RLPDYYRTDISANYNFKIGEARIKAGATIINLFNVDNYYDVNSRKFDTENASFSETNV
                     VRAQDRSLNLFIHFVF"
     gene            31797..32762
                     /locus_tag="PK252_02620"
     CDS             31797..32762
                     /locus_tag="PK252_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13632.1"
                     /translation="MKKKLLILVCAMAMVAFAGCQKSDEKSENISALNSLSENVAIAA
                     TSVSTSQMALSCSEDIQATVLSDVDSSYRKVKSYFHRYGKGAFKLCLSNIPICATVTV
                     SSDTFPKTIIIDYGTSGCTSQHGNVKTGKIIIEVTDTIINAGAVKSIKYVDFYVNGNK
                     IEGSGIIQNLGKNSNGNWVLATNTEFTFTDSSNNVAKEIYNDTIEWVGGFETADKSDD
                     VYYKSGAGSITLNDTIAYSRTITTPLLMNNTCGYIISGVETLYKNGSTVVIDYGDGTC
                     DVVATVTINGTTEEISIEKHHFKKGGHFEKQVGHSKGNKKGLFGF"
     gene            complement(32811..33875)
                     /locus_tag="PK252_02625"
     CDS             complement(32811..33875)
                     /locus_tag="PK252_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOT13633.1"
                     /translation="MKRLLTICCIALSVNSLNAQQETFKEQLPENVILMIGDGMGIAQ
                     MYAAYTANGQYLNIFGMPVTGIMLTYSADEYTTDSAAGGTAFACGKKTNNGSLSVDTA
                     GKPMQTIFEYAKEKHYSTGIAVTCAITHATPAAFYAHVGKRSEYETIASHFLNKTVDV
                     AFGGGRKYFVKRKDKRNLIELLMKDGYFYSTNIDSVDISQQKVLCLATDGDLPKISEG
                     RNDFLPLATQKSIELLKKNRFLLMVEGSQIDWGNHLNSINYVINETVDFDKAVKVALD
                     FAKKDGKTLVIVTADHESGGLTLINGNFKKSTVDSNFSSLNHTAVPVMVFAYGPGAEM
                     FTGCYQNTALFDKIKMLISK"
     gene            33972..35024
                     /locus_tag="PK252_02630"
     CDS             33972..35024
                     /locus_tag="PK252_02630"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOT13634.1"
                     /translation="MGLNIVKDYFQKSGKEQNIMLFTLKNTEGTEVAITNYGATITSI
                     KTKDKNGKPGQIVCGFDNIEDFQGNHPFFGVVCGRYANRIAKGKFTLEGKEYTLAINN
                     APNTLHGGNKGFDKVVWSFEVVESAQAVGVKLTYVSKDMEEGYPGTLTATVTYLLNEN
                     NELQIHYTAKTDKTTVVNLTNHAYYNLGGFAGEITDHLLQINANSYTETDSTSIPTGK
                     IIELAGTAYDFRELTLIGDRIKGTTNGYDDNFILNKAGQPACSFAAKVVHPGTGRTME
                     VYTTEPGVQFYSANYLDGTIKGHDGVVYKKRFALCLETQHFPDSPNHAHFPTTVLKPG
                     ETYTQTTIHKFGVTEK"
     gene            complement(35130..35576)
                     /locus_tag="PK252_02635"
     CDS             complement(35130..35576)
                     /locus_tag="PK252_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT13635.1"
                     /translation="MRSIISLLFVFTMLTFISSCKTKINKEAVKEEIFKTEKAFEAAT
                     KSIGVAEAFGEFADDSAVIIRGENNLIKGKAAIKQYYEENTAKNAEVSWAPDFIEVSD
                     DGTLGYTYGKYTWKSADSFGKTTESKGIFHTVWKRQADGSWKYVWD"
     gene            35746..36741
                     /locus_tag="PK252_02640"
     CDS             35746..36741
                     /locus_tag="PK252_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOT13636.1"
                     /translation="MMQNQEIAIIGGGSWATALASIVSHNVKKFHWFIYEKEIIDHIK
                     THKHNPQYISAIEFDPSKIVFHNHVKEAIDAANVIIFVVPSAFIKPLMGKEDIDFSGK
                     FFVNAVKGLIPDDNITVTQYFQEQFSIPFSRVGVVSGPCHAEEVALERLSYLTVGAEF
                     EEDAKQVADLIRAPFVNTIISNDIYGIEYGSVLKNIYALGAGINHGLGYGDNFQAVYV
                     SNAIREMERFLGKVFERERHLFHSVYLGDLLVTAYSQFSRNRTFGNMIGKGYSVQAAM
                     IDLNMEAEGYYATKSIFEVNKKFKVKMPIAEAVYKILYEKCNAAAEVKALAQKLK"
     gene            36769..38124
                     /locus_tag="PK252_02645"
     CDS             36769..38124
                     /locus_tag="PK252_02645"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT13637.1"
                     /translation="MSTLNLSIDKIFDFVSKDKVYAFADECVKQNKALHEKTGKGNDF
                     LGWVNLPSSITPEIIKDIEKTAASFRKNCEVVVVVGIGGSYLGAKSVIEALSDSFYQI
                     NRPKDTPLVVFAGQNIGEDYTAELIKLLDKKKYGIICISKSGTTTEPAIAFRILKGHL
                     EQKVGKPHAKELIVAITDAKRGALRTLATQEGYKTYVIPDDVGGRFSVLTPVGLLPIA
                     VAGFDIAKLVAGAQKMEELTAPDVAFEDNPAAIYAAARNALYKAGKKIEILANYNHKL
                     TFVAEWWKQLYGESEGKENKGIFPASVTFTSDLHSMGQYIQEGERHIFETVITVKNTN
                     HNVAVPFEKDNLDGLNFLAYKRIDEVNKMAELGTLVAHMDGGVPNIIIEISRLDEYNL
                     GLLFYFFEKACGISGYLLKVNPFDQPGVEAYKKNMFALLDKPGFETDSEILKKRLGIN
                     K"
     gene            38197..38853
                     /locus_tag="PK252_02650"
     CDS             38197..38853
                     /locus_tag="PK252_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT13638.1"
                     /translation="MLEVKNIHKSFGKLEVLKGIDLSIKKGEIVSITGPSGAGKTTLL
                     QIMGTLLKPTSGKVFIGGKDVFAMKDDQLAGFRNSEIGFVFQFHHLLPEFSAVENVII
                     PALIARKKKKDAIEKAKSLLEELNLGHRLNHKPGELSGGEQQRVSVARALMNDPLLVL
                     ADEPSGNLDSVHKTELHQLFFDLRDRYNQTFVIVTHDNELAAKADRQIKLADGLVIAQ
                     "
     gene            38837..39634
                     /locus_tag="PK252_02655"
     CDS             38837..39634
                     /locus_tag="PK252_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOT13639.1"
                     /translation="MLLPNNLIKDLQEIKQSLDWLVIHFNTPAFIEKDPISVPHRFSK
                     KEDIEIAAFLAATIAWGQRTTILNNANKLMGLMDNAPYDFVTGHTQKDLQRLNGFVHR
                     TFNEVDCIFFIKSLQNIYSRHNGLEGVFNTGFHHDNTVFSALVYFRKLFFETEHQYRS
                     EKHISDVTKGAAAKRINMFLRWMVRQDKCGVDFGIWRNIPASALMLPLDVHTANSARQ
                     LGLLVRTQNDWKAVEEIMSVLRKFDPHDPVKYDFALFGASIEKIFLK"
     gene            39649..40359
                     /locus_tag="PK252_02660"
     CDS             39649..40359
                     /locus_tag="PK252_02660"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT13640.1"
                     /translation="MANSYFQFKKFTINQEKCGMPVNTDSVLLGAWANVDNCNKILDI
                     GTGTGILSLMMAQRCNATIDAVEIDEASYLQALTNFEQSDWGDRINAYFCSIQEFAAS
                     TSSKYELLICNPPYFANAVKSTNHQKKTARHTDTLSFENLISATMHLLEDKGRLSLIL
                     PYSEGCIFIALAAKEGLFCVRKTNVKSVTHAPITRLLLEFATLPQQVAEDYLVIADEQ
                     NNYSDKFRHLTVDFYKFF"
     gene            40476..41732
                     /locus_tag="PK252_02665"
     CDS             40476..41732
                     /locus_tag="PK252_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT13641.1"
                     /translation="MKNRAITIIVVLSSIAMLGLIAAQAYWVNKAYDLSQKHFEHRVY
                     SGLYGAMLEISWNKKPMPKDSANMTEAQILLARVRPGSVDTSLYRHFGYNQVDNKYEF
                     AIVLNSNDSIVYQSPGYVRDVKKEKVFKKCLWYMPSMKNFSIRVIFPQIQKTLVGDIW
                     FWLFLSGFFLMVVILCFAAILILAYKNKKLSEMKTDFINNITHEFKTPISTISLASEI
                     LVNVNQASHLDKVKRYASIIQEENRRMQMQVEQVLRMSKLDKKEYELNKENIDLNELV
                     TNAVQNLCLDLDNPAIKIHFSLSDKKIEMLADPVHLTNIIKNLVDNACKYSKGNPQIT
                     VCTSLNNTYATISVADKGIGIEHDKQKYIFEKFYRIPTGDVHDVKGSGIGLYYVKVMT
                     EAHGGKVIVKSEPGKGSQFDIVLPLK"
     gene            41750..42451
                     /locus_tag="PK252_02670"
     CDS             41750..42451
                     /locus_tag="PK252_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT13642.1"
                     /translation="MKNKGNILLVEDDKNLGFIIKDFLHESGYHVDLAENGQSALAIF
                     GTKSFDLVLLDVMLPIIDGFSVAETIKAKNAIVPIIFLTARSLDDDKIRGLKIGADDY
                     ITKPFSTEELLLRVEAVLRRTKGHVANVVVSQEIFQIGSYTFDSSNQTITLGDDVKML
                     TRKEADLLRLLCINKNQLVRRDFALKLIWGEDDYFMGRSMDVYITKLRKILKDDPSIQ
                     IQNVHRTGFKLEVKD"
     gene            complement(42455..42712)
                     /locus_tag="PK252_02675"
     CDS             complement(42455..42712)
                     /locus_tag="PK252_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT13643.1"
                     /translation="MGGSEILLILLAILVLFGADKIPGFARSLGKGINEIKKASDEIK
                     EEIARETSHLKKEVQDTTNEIKENSAIIEENKDKANHGYPF"
     gene            complement(42795..43265)
                     /locus_tag="PK252_02680"
     CDS             complement(42795..43265)
                     /locus_tag="PK252_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT13644.1"
                     /translation="MKVLLLVTGKTDFTFVSQGIQLYTERISRYLNFQVKEVTQKKGN
                     VADTQVIKRQEASLLLQQIEPTDKVVLLDERGNQYTSPEFASFIQGQMNSGCKRLVFI
                     IGGAYGFDESVINRAQYKISLSKMTFSHQIVRTIFLEQLYRAFTIINNEPYHHE"
     gene            43458..43688
                     /locus_tag="PK252_02685"
     CDS             43458..43688
                     /locus_tag="PK252_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13645.1"
                     /translation="MFEKELEIFKKIKADLLSQYSGGFVVIKGDEVLGVWQSRLDALK
                     SGIDKYGDVPFLVKNINESDIVINFSRNLIFA"
     gene            43691..44119
                     /locus_tag="PK252_02690"
     CDS             43691..44119
                     /locus_tag="PK252_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOT13646.1"
                     /translation="MPVFPNRVPNLQQIGPIVEVVIFPSQPIYIKMRNEGQNPPSKKV
                     IALIDTGASGSCINDPIAKELGLIARDVVPVQTPSGTSQQPIYDLGFSLPSLTANIIP
                     IQALGSDLEKQPYDALIGRDILSFCTLIYNGWDNSYQLHI"
     gene            44156..45007
                     /gene="nadC"
                     /locus_tag="PK252_02695"
     CDS             44156..45007
                     /gene="nadC"
                     /locus_tag="PK252_02695"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT13647.1"
                     /translation="MEIRKEDILPFLKVVIAEDLGDGDHTSLATIDPEQREKAQLIVK
                     QEGIIAGIDVARMVFELLDSTVTFTPLMQDGRQVKFGEIAFIVEGRTQTMLQAERIVL
                     NIMQRMSGIATQTNTYVKKLEGTKTKVLDTRKTTPCMRLLDKYAVKIGGGENHRIGLY
                     DMILIKDNHIDFAGGIEKALEKTYQYLKEKNKNLKVEIEARSLSDVQRILNFGRVDRI
                     MLDNFTPALTKQAVELIAGKAEIESSGNITIENIRDYAECGVDYISVGALTHQIKSLD
                     LSLKAIK"
     gene            complement(45031..45888)
                     /locus_tag="PK252_02700"
     CDS             complement(45031..45888)
                     /locus_tag="PK252_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT13648.1"
                     /translation="MPKDLKQKIKSHAIITFGLLLTAIGWMAFLIPAQITGGGLSGLC
                     ALLFYAIKLPIGLTYLILNVILILISIRILGLKFGIQTVYGVIGLSVFFTILQPFFTA
                     PIVKDAFMATVIGGILSGAGVGLVFSQGGSTGGTDITALIIHKYKNIRLGRLILYQDV
                     IIIGSSYFLFYSLEKIVYGYVTMAITSYVIDLILEGTNQSYQILIFSKKHEQIAQRVS
                     DEVGRGITLIKGQGWYSKQNMDILLIVVRKYESAAVLRITNEEDPQAFISMSSVMGVY
                     GEGFDRVKI"
     gene            complement(45955..47058)
                     /locus_tag="PK252_02705"
     CDS             complement(45955..47058)
                     /locus_tag="PK252_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT13649.1"
                     /translation="MDKTTLFIIIVTTSLILFAVIKRIVFKRSFVLNATHSITASNVI
                     IICIAYYIGTSSLTHAFWAAPLVLLTILISYIMMRNIMRKPFEMIRSQLEALAEGTIE
                     NEDLKYDKEDEIGDISKALNRHKSTLRRLVTQIGETSKNLSEASQALTSDAENLASLA
                     SEQAATAEMVSSSVEEMSAHLAQTHQNAANTDKVAKTSVEKLKEVSTVSSESFSNMEI
                     IKQRITIINDIAFQTNILALNAAVEAARAGEHGKGFAVVAAEVRKLAERSKIAADEIQ
                     TLVQQMVESTSKTNQLLIDLIPNIDNNSSFMQEISSACSEQLIGIQQINNSMQELNNS
                     SQETVVVSERLSESSTLLADRSKDLITTIDFFK"
     gene            complement(47238..49169)
                     /locus_tag="PK252_02710"
     CDS             complement(47238..49169)
                     /locus_tag="PK252_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOT13650.1"
                     /translation="MKKIIVLCLLGLSLACSQKVSDRYTIKSPDGNIAVTISIDSLGS
                     LTYNLQCKDSLVLESSKLGLKLSEADFTTGLTIVGTSEVEKVKDIYQLKNEKREKCSY
                     NAIKQRIDVKNQDGEMLSVVFQVSNDGLAFRYEINKYKNQSDSVIVENSGFNFPLSAK
                     AWLHPQTDAHTGWNFDQPSYEQYYYTEVPVTTKAPYKAGWAFPALFKSAGNWVLISEA
                     DVDRNYCGSRLSTASPDAEFSIQFPQPMERTDSLAAVKPQITFPFVSPWRVIIVGQTL
                     GTIVESSLITDVSKPCVLKDTTFIKPGKASWSWVLYKDDSTIYSTQKRFIDYASQMHW
                     QYCLIDAFWDKKIGYEKIADLVAYANSRNVKIILWYNSAGSWNTTPLTPRNLMFDKAI
                     RNKEFEKISKMGIAGVKVDFWGGDGQSMIAYYHDLIEDAAKYKLLVNTHGCTVPRGWS
                     RTYPNLVAMEAVKGFEFCTFTQEGADMQAAHCAMLPFTRNVVGPMDFTPMCFSEIPNL
                     KRNTSNAFEMALSVLFQSGIQHYAEVPEGMAKQPEFVKDFIKTLPSSWSDIKFIDGYP
                     GKYVVIARKGNDKWYIAGINSTSEDLNLKFDISGFKQKKDAVIITDGKTNRDFRTENT
                     NNNEVKLQVKPMGGFVIEL"
     gene            49352..51109
                     /gene="aspS"
                     /locus_tag="PK252_02715"
     CDS             49352..51109
                     /gene="aspS"
                     /locus_tag="PK252_02715"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT13651.1"
                     /translation="MYRTNTCGELNLSHVGKNVTLSGWVQRARDLGGMTFVDLRDRYG
                     ITQLAFNMDKNAELCLEARKLGREYVIQVSGLVAERESKNLKMPTGEIEIIVDSLKVL
                     NTADVPPFTIEDVSDGGDELRMKYRYLDLRRNPLKQNLMLRHRLAQEVRRYLDSKNFM
                     EIETPVLMKSTPEGARDFVVPSRMNQGQFYALPQSPQTLKQILMVAGYDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVDRDDIIETIEGLMKYLFKTVKNIEFKDAFPLMPYS
                     EAMNFYGCDKPDLRFDMRFVDITSIAKGKGFGVFDNAEYIGAICAKGCSEYSRKELDA
                     LTDFVKKPQIGGSGLVYVKYGADGSIKSSVDKFYTPEDITVWLNAAKAEKGDLLLIVC
                     GPKEKTLKALGELRLEMGNRLGLRNPDVFVPLWVVDFPLLEWDEESKRYFAMHHPFTA
                     PVDEDVPLFDTDPGKIRAKAYDLVINGVEIGGGSVRIHSKDLQSKMFNAMGYTQEEVD
                     YQFGFLLNAFRYGAPPHAGLALGFDRLTSMFAGLDSIRDVIAFPKNNAGRDVMLDAPS
                     VIRDEQLKELGIAVVAKKE"
     gene            complement(51443..52858)
                     /gene="pyk"
                     /locus_tag="PK252_02720"
     CDS             complement(51443..52858)
                     /gene="pyk"
                     /locus_tag="PK252_02720"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT13652.1"
                     /translation="MKKTKIVATISDKGCSVEFIRQLYEAGMNVVRLNTAHQGFEQAQ
                     KVIDIVRQVSDKIGILVDTKGPEIRTTPSKGFAVKTGDVVKIAGQPEKETVPECIYVN
                     YTGFVHDVPVGSHILIDDGSLELCVKSKNEEYLVCEVCNDGKVEGRKSINVPSVSFRL
                     PSLSDKDRDFVKYCIEQDIDFIAHSFVRNRNDVMAIQEILDKHNSKIKIIAKIENQEG
                     VDNLDEILDCVYGVMVARGDLAIEIPYEKIPGIQKMIVSKCIARRKPVIVATQMLHSM
                     IENPRPTRAEVSDIANAIFSKTDAIMLSGETAYGKYPLQAVETMARVACEVEKSRSDI
                     HDVPMVVLSNQRSAYLTKTAVEAAITLNAKAIIADSETGRSIRNMAGYRGRKPIFAFC
                     YDKRTVRELSLSFGVFADVIEESNSSYEFVHKALSHCLEEKMLEKDNLVVVIAGNYGS
                     NFGASFIEISPVEKLLNRHTR"
     gene            53045..53947
                     /gene="xerD"
                     /locus_tag="PK252_02725"
     CDS             53045..53947
                     /gene="xerD"
                     /locus_tag="PK252_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT13653.1"
                     /translation="MTWEEANIRFVNYLKIERSLSANSVEAYDDDLHKLRQYFEYINA
                     SVVPEKVTTGQIQGFLQWINDLGMGARSQARVLSGLRMFYKYLIFDGVIDNDPMELVE
                     SPKIGRKLPVVLSIEEIDALIAAIDLSKPEGRRNKAIIETLYSCGLRVSELIELRYSN
                     IFFDKGFIKVLGKGNKERLVPISNKAVKEIQQYLPDRNSMLDVMTKDSENILFLNNRG
                     HKLSRVMIFTIIKNLAEKIGLKKNISPHTFRHSFASHLVEGGADLRAVQEMLGHESIL
                     TTEIYTHLDRQYLRDTIIQFHPRG"
     gene            53999..54631
                     /gene="plsY"
                     /locus_tag="PK252_02730"
     CDS             53999..54631
                     /gene="plsY"
                     /locus_tag="PK252_02730"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOT13654.1"
                     /translation="MILFVSLFLLAYLLGSIPTSVWVGKWFFGIDVRQHGSGNAGATN
                     TIRVLGWKAGGPVFVVDIAKGFAAASLVWATELMPKTSQFVNFQLILGFAALLGHIFP
                     VFAGFRGGKGVATLLGVVIALSPLPAVLAFMVFLVILFIFKYVSLASMTAGLSYPLML
                     WFLFPTLPLSLKIAGIVMSLALIATHKKNILRLIKGEESKAYFLIGKKNK"
     gene            54652..56550
                     /locus_tag="PK252_02735"
     CDS             54652..56550
                     /locus_tag="PK252_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT13655.1"
                     /translation="MTEQKIRFGLVFLLVSIFFGLTAIGQGIVVERSSERIMIKGKVY
                     FIHQVKKSETLYSIAKAYGISLDTIYAYNPGTTEGIKIDQLLRIPDYKSKGTVYESRH
                     EVEIPENIKEPAKVQHTIVAGQTLYGIARIYGLKPSDIEKANPGMIRDSLKPGQQIII
                     PLAQLKKDSASKNFFIHKVQEKETLYSISRLYKTSVDSLKIINGLSNDSILRLGMELK
                     VPMVVGVVQQKDMAQTSAETSTPVVISMDCDSFLAKTEQNQVNLTLLLPFFASKAEGG
                     GEDENVAEEKRHNRQKSMFSPQSASYVEFYQGCLLALEDLKRKKIDVNLQVYDTENDT
                     LLLHSIMKKPEFQSANVVIGPVSLSLMNYASRHMQKNGTYWVSPFADITNITKGNPFV
                     FQVNAGKENLFLENIKHIPDNDTNKVIVVYNGSESDINDDFRNAAAKSKDLKVASMWK
                     ELNVYHNGFAELVSMLDSTKENIIISPSVDEIFVSNLLSILESKLLLYRIKVIGRTEW
                     TLFSSIDMNYLFDQQLSYNTPFYIDYESEPVKRFLKKYRLYFGTEPGQMSKQGFNYAM
                     LGYDVTYYFTMGYAQYGNKLPLATLCFNPEMLITQFRFQKVSPVGGMINSTLHKVSYT
                     RDYNVLHE"
     gene            complement(56558..57316)
                     /locus_tag="PK252_02740"
     CDS             complement(56558..57316)
                     /locus_tag="PK252_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOT13656.1"
                     /translation="MLENIKILWHNGKQYIPDVTTAKVPGIFKGRPVIASNIKVDEDK
                     LTALCPVKAIGLNPVSIDLGKCVFCGECALAFPQKIRFTTDYKIATNVRERLIVKEGD
                     ENPVTLDQSIIRKEIKQLFSGSFKLRQVSAAGDNSCEWELNASNNVQFDISRFGIDFV
                     ASPRHADGILITGPISANMAEPLERCYNATPDPKVIILAGTDAISGGIYADSPALERS
                     FLEKYPIDLYIPGNPAHPLTIINGIVDLIRKKKG"
     gene            complement(57329..58834)
                     /locus_tag="PK252_02745"
     CDS             complement(57329..58834)
                     /locus_tag="PK252_02745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT13657.1"
                     /translation="MTNKFISVKNNEPVPVKTIPELTYKEFTDICTSLVKDKIHCHIV
                     NYFGYRENNNIKLICCIADDQEQSINVLSTNVETDASYPSLTIDVPAFDKFEREVYEN
                     FGVKFEGHPWLKPVRYPFNRANKQQTIENYPFLKGSSEELHEVGVGPIHAGVIEPGHF
                     RFICHGEQILHLEIQLGYQHRGVEQLMLQKQSLNERITIAESIAGDSAVEHATAFAQL
                     WESLCNIETDKSTAFGRSLAQEIERIAIHTGDMSAICTDIAYQLGSAVFGRLRTPMVN
                     FFQEWCGNRLCKGLIRVGGFNYPFTEALGKRFLEILTIYERDFEEMATQLFKLPSALG
                     RLERTGVVENDQIYQIGAVGMAARMSNLKRDIRMSHPYANYKNMNHQPVVKRHGDVYS
                     RTQIRKEEIRQSIGYIRNWINNIPEFKKVDASNYTAQADTFVISLTEGWRGEVCHCAI
                     TDNKGMLKVYKIKDPSFHNWKALELAVRNNEISDFPICNKSFNLSYCGHDL"
     gene            complement(58831..60264)
                     /locus_tag="PK252_02750"
     CDS             complement(58831..60264)
                     /locus_tag="PK252_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT13658.1"
                     /translation="MISVFLILSSLIGIGLFFNKNKSLMYSLLVLYVIQLWVFGISQY
                     LQIHTEASFTYFKPDHIGILLLLALAIVAIPSLFHGYRYMEKHNEGETPRTKGLFFSA
                     LIFLMTASAAAYLSNHLAVTWIFVEATTLSASMLIYHHRNIRALEGVWKYVFICAISI
                     TFVFIGILFLSLSFKSAGITDLTFDSLYANYAKADLFWLRLSFIFIFIGFTAKLGLVP
                     MYTAGIDAKDKAPAPAGALFASILMNVGFISIYRTYALINKTPLQHWAKTVILIAAVL
                     SIFVAAVYMMRIKNIKRMFAYSGIEHMGIVMIGIAAGGIGVYAALLHIVLHTLVKPVM
                     FFQYNQIYWVYQSKSIYDIGNYFKYNKTGAIVVLLGFLSVTAIPPSGMFVSEFLIFQS
                     LIAQKLYLILILMFILLTIIIYGFGSNVFKLLFTPPIDFNEERVPVIKPWESLSQYIL
                     LGLAIWMAYCPPSILIELINGSLKLIA"
     gene            complement(60270..60899)
                     /locus_tag="PK252_02755"
     CDS             complement(60270..60899)
                     /locus_tag="PK252_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13659.1"
                     /translation="MTDILLIALAMSLLYMSIANRMITYIHILLFQGILLFAITYLTL
                     DKVEPLNVAFIMLETIVFKAIAVPWFLNSLIKRNNITREAEPFLPNFLSLIIVTLIIV
                     ISIILSSSVSSKLIDKTFFIVALSTLFSGLYLIVSRRKIITHVIGYLIIENGVFILSL
                     AVGNEMPMLVNLGIMLDIFASVLILGIFFNKIGDVLKDPDVDLLRNLKD"
     gene            complement(60905..61810)
                     /locus_tag="PK252_02760"
     CDS             complement(60905..61810)
                     /locus_tag="PK252_02760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT13660.1"
                     /translation="MISFILIVLVSIVFSGVIIRTKSLASGRKGPGILQPMKDIVRLW
                     RKGAVYSKTTSFVFQIAPSIYFASLIMAALLVPFGGEKGILSFQGDFVMFAYVLALGK
                     FMMIISSLDTGSSFEGMGASREALYSMLTEPAFFILMGSFALFTGQSSFADIFNNLHL
                     RSENAYMIGILAAFVLMFIAMIENSRMPIDDPKTHLELTMIHEVMILDNSGFDLGLIL
                     YSAQLKFAIYGALIANFFITSMPFTIAIPIFLGIQVLFAFIIGLFESFMARFRMNHNP
                     QFVFILTSVAMLIFLGVLLVLGKLI"
     gene            complement(61807..63852)
                     /locus_tag="PK252_02765"
     CDS             complement(61807..63852)
                     /locus_tag="PK252_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT13661.1"
                     /translation="MIKPGFEILNIIGLAAVVAIPLVGSKANKMMTSVVFCLLFSLSI
                     ALSVLVFYNGTQIFTYSGNIITGPITLKADYLSAWFISIIAFVFTIGYFYSKGYLKHY
                     PERTSETKLHYAAFILGLISLIDICLIQNSLVFLFVWEIMTISITVLVIFESHKSEVL
                     RAGMYYFIVSHIGLLMLFVAFLWGKANTGSYDFEAMASYMLSAPNSTKLIIYGLFFIG
                     FGIKAGIFPFHVWLPVAHPAAPAHISGIMSGVVIKIGIFGIIRMLFIVHPDFCITGYI
                     ILFLSLVSGLYGVMLAIVQHNLKKMLAYHSIENIGLIGIGIGIGCLGIAYNMPLLFVL
                     GFSGALLHVWNHALFKSLLFFCSGNIYQSAHTMNIDRLGGLNKQLPKTTMLFMSGAIA
                     ICGIPPFNGFVSEFMMYVGLYNGLQGGYFILSFWALFGIIIMALIGGLALMCFTKATG
                     IIFLGNKRETKLEYKNTDNGNMIYPLFLLAIAIAIIGFAPFLLNKTLFSLISSLKVHT
                     SQFVIYQPEIETYLREATHIGWFSICFILIIATVIILRKAIVVNKVKKEDHTWGCGYT
                     GETVAMQYSGSSFIREYRKLAEFIFKTIKKKKAAKGLYPSKIEHSTHILDYIEIWLID
                     KPIKLLRLALNRFTFLQNGNIQVYLLYGLVFIMIILIYTSINQNIDTLLNFLKTKAL"
     gene            complement(63868..65862)
                     /locus_tag="PK252_02770"
     CDS             complement(63868..65862)
                     /locus_tag="PK252_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT13662.1"
                     /translation="MELISIIAICFIIVLINALFTGLLSNKGQDILLFLTLLTGIILT
                     SIPSLSAITGYPIVEKFLLPHNLGTLNIQLDSLSAWLVLIINFGVLQGSIYGIFYLKS
                     YQLPTNKWIHYMTLPLFHLSMIGVCVFQQGFGFLIAWEVMSLTSMVLVMFEYQKRISI
                     RAALNYLVQMHVGVLLLISAFILLFINTGSSDFSNIALLNNKHIVLVIFGLFFAGFAI
                     KAGFVPFHTWLPIAHPAAPSHISGMMSGLLVKLGIYGIIRVVLYLPMEYTTIGTFILV
                     LSLVTTLFGIANAGLRSDFKRMLAYCTIENIGIIGIGIGIGLIGKGINEHLISWLGFT
                     AALLHILNHSLFKTLLFYSAGSVYRQTHSKNIEHLGGLIKFMPYTAAIFLIGGIAICG
                     IPPFNGFVSKFVLYKSMIAGLSYSNTEISMLFILSMAVLAISGGISIFTFTKNFGLIF
                     LGNTRKKLEHQPKEVNWAMLLPQFIIIAAMLSIAFFPSFYISLVSDSSNKLSTIYNIS
                     NSISACSPHILSNVALCSACFILIIGLVFAIRQALTSKLPETTFETWGCGYTGPAKAT
                     QYTGRSFSETLAKLLPIIPSQKLLEPKERKGIYIQKLKFSIRYHDIFDVYIIEPFTNL
                     VNRMISLFRFIQNGQVQLYLLYGIVFLLIILIGSFWNIIR"
     gene            66240..66938
                     /gene="clpP"
                     /locus_tag="PK252_02775"
     CDS             66240..66938
                     /gene="clpP"
                     /locus_tag="PK252_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOT13663.1"
                     /translation="MDIKQEFEKFAKGKKGISSLTMHRFESMYNSYISPTIIEERQLN
                     IVTMDVFSRLMMDRIIFLGLPIDDYVANIIQAQLLFLDSVDSGKDIQIYFNTPGGGVY
                     AGLGIYDTMQFVNSDVATICTGMAASMGAVLLTAGTKGKRSALKHSRIMIHQPMGGAQ
                     GQASDIEITAREIQKLKKELYEILAEHSGNPIKKIEKDSDRDYWMTAQEAKDYGMIDE
                     VLERKKINTVDEGF"
     gene            66925..68160
                     /gene="clpX"
                     /locus_tag="PK252_02780"
     CDS             66925..68160
                     /gene="clpX"
                     /locus_tag="PK252_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT13664.1"
                     /translation="MKDSKNHCSFCGRSKNEVNLLITGAEAQICDICVGQAGEILRDE
                     VKGPKSKFDVSKVKLLKPIEIKAFLDQYVVGQEEAKKFLSVAVYNHYKRLIHANKRVD
                     NDVEIEKSNILLVGRTGTGKTLLARTIAKMLHVPFTIVDATVLTEAGYVGEDVESILS
                     RLLQSADYNVEAAQRGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVN
                     VPPQGGRKHPEQKLIAVDTRNILFICGGAFEGIENKIAQRLNTKVVGYTASVKKTRLD
                     KNNLLQYVAPQDLRAFGLIPEIVGRLPIVTYLNPLDKATLRRILTEPKDAITKQYVKL
                     FEMDNIKLVFDEEALDYIVDKAVEFKLGARGLRSICEAVMIDAMYEMPSEERKELHIT
                     LDYARKNIEKANLGELRAA"
     gene            68172..69074
                     /locus_tag="PK252_02785"
     CDS             68172..69074
                     /locus_tag="PK252_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13665.1"
                     /translation="MEKTFKYQVFGLNIISEIELPIPEKSFDTAQVEIRFGETPFTLG
                     GEARRGVVYEALPNAVLIKVFDIARFHIVDGSAIIVQPLEKYDNKSAVAFIMGSVFAS
                     LLYQRGIMPLHASALEYKGKIIVICGVSGAGKSTLALGLAKQGFRIICDDIVPIYFQN
                     GLPMASSGFNQSKIWKDTAAIMDISVEPLVKVREEIDKFYYEAENVLLTNEPLSVKHI
                     FILSATNKENLETKNIEGKDKFLVLRNHLFRKQIAFSMGLEARLFGSLAKLAASVPVT
                     KIERPTKGFSLEIPQNSILQILDL"
     gene            69078..69914
                     /locus_tag="PK252_02790"
     CDS             69078..69914
                     /locus_tag="PK252_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HOT13666.1"
                     /translation="MGNINWLASYPKSGNTWIRIFLANLMANNAQPVNINQLQDFSIA
                     SNRETFDELSGLSSSDLTLEEIERLRPLVYEIQSDNATEKIFLKIHDAYIYTCDGIPL
                     VSTRATNKALYIIRNPLDVAVSFAHHLGATHEKAVENMCNEHFAFCNKNLKLHKQLEQ
                     KLLSWEKHVLSWTSQQNIPVKVVRYEDLLHFPEITFTSIVEYFELDYGKSQILSAIQN
                     SSFSELKKQEETHGFIEKSPQAKSFFRKGKAGLGKDELGAELSNKIIASNERVMRQFG
                     YL"
     gene            complement(69972..72011)
                     /locus_tag="PK252_02795"
     CDS             complement(69972..72011)
                     /locus_tag="PK252_02795"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT13667.1"
                     /translation="MNNTVVNKAADNIRILAAAMVEKAKSGHPGGAMGGADFINILYT
                     EFLRYDPSDMTWHMRDRFFLDPGHMSPMLYSVLCLAGNYTLDEVSNFRQWGSPTPGHP
                     EVDVKRGVENTSGPLGQGHVMGVGAAIAERFLVARFGEWMAHKTYAYISDGGIQEEIS
                     QAAGRIAGFLGLSNLIMFYDSNDIQLSTETKAVTIEDTAKKYEAWGWKVVTIDGNNAD
                     QIRQALKAANAETEKPTLIIGKTIMGKGAMKADGSSFERNCATHGMPLSEAGASFEKS
                     IESLGGDPKNPFVIFPEVKELYSNALEQKKKEAAERKKQQAEWEKANPEKAAKLKLFL
                     SGQLPQLNFESIAQKENIASRAASAAVLGYLAQNVENMIVASADLANSDKTDGFLKYT
                     KAFTKGDFSGAFLQAGVSELTMAAICNGIALHGGVWAACATFFVFSDYMKPAVRLSAL
                     MQLPVKYIWTHDAFRVGEDGPTHQPIEHEAQLRLMEQLKNHHGKNSLLALRPADAYET
                     SVAWKMAMENTATPTALILSRQNIKDLPASGSRYKEALQAEKGAYIVVNCEGTPDVVL
                     VASGSEVATLVEGAALLKAQKNLKVRVVSVISEGLFRTQTKEYQQSVIPAGIPVFGLT
                     AGLPVTLLGLVGEKGKVWGLDHFGYSAPAKVLDEKFGFTADNVSNQVVKFLAEYK"
     gene            complement(72241..74484)
                     /gene="bglX"
                     /locus_tag="PK252_02800"
     CDS             complement(72241..74484)
                     /gene="bglX"
                     /locus_tag="PK252_02800"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOT13668.1"
                     /translation="MIKKLILMATAILIVSCQQQQTGQDSEIENKVEDLLSKMTLSEK
                     IGQMNQLSSSGNADDFKEMLKKGMVGSFLNEVNPVTINKMQKVVMEESRLKIPLLFSR
                     DVIHGYKTIFPIPLGQAASWNPDIIEKGARVAAQEASEAGIRWTFAPMIDITRDPRWG
                     RIAESLGEDPVLTSVLGAAMVKGFQGKSLNDTNSLAACVKHFCGYGATEGGRDYNTTI
                     IPEQTLREVYLPSFKASIDAGAATLMVSFNEINGVPSSGNDFLLRQVLRKEWNFDGMV
                     VSDWGSVEEMINHGFCADRKEAAMKAINAGVEMEMVTSTYVEHVENLIKEGKIKESTI
                     DEAVRNILRLKFRLGLFDKPYVAVNSKSSLYSDEHLASAKEAAIQSLVLLKNENNTLP
                     IKSTVKSVAIIGPLADAPYDQMGTWTLDGEQEHTITPLVAIKEEFGKQVKVNYAKGLE
                     YSRDLSTKGFTAAISAARQSDVILMFAGEESILSGEARCRADITFPGKQRELIAELKK
                     TGKPLVLVIMAGRPLAITEESKIADAIVYAWHPGTMGGPAIADVLFGKVPPSGKLPVT
                     FPQMTGQIPVYYSQKNSGRPAKEPLMLLNDIPVSAKQFTLGCSSYYLDAGYKPLYPFG
                     YGLSYTTFVYSDLKLSKNQMKKEDQMEISCKITNTGNVEATEIVQLYIRDLVGSLTRP
                     VKELKDFKRVAIKPGETVKITFTLNTNQLKFWNAKMEQVVEPGQFKVWIAPNSSEGLE
                     GSFEVTE"
     gene            74732..75511
                     /locus_tag="PK252_02805"
     CDS             74732..75511
                     /locus_tag="PK252_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13669.1"
                     /translation="MNAVLRIIFFVSLVIVGFSACTVREVISVSHSARDNVTIDGIAL
                     GTASYSKLFGVETSSADACVLQARLAMYRAFPLQPNQAFDNMVVEKKTTYFPFYSKTR
                     FLISADIITPEIDSKNRFAYSPSFENMLFSNKKIEHEGLQLADSVVFKYKGKYTKGVI
                     LDFSDKSVWLGLNFKNQLRIVKASVNKVFAIGNEELEDELGLETGQIIEISYVSTNNN
                     QDVCSKEIVGMNKYHVLILQKNGTDYSYQVVAANKIKEKKH"
     gene            complement(75517..76686)
                     /locus_tag="PK252_02810"
     CDS             complement(75517..76686)
                     /locus_tag="PK252_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT13670.1"
                     /translation="MANILVIDDERGIRNTLRDILEYEKHTVETAEDGLMALEILERK
                     EFDLILCDVKMPKMDGLELIEKIFEKAGDVQIVMISGHGNIETAVEAIKKGAFDFIEK
                     PLDLNRLLITVKNALDKTHLITNTKVLKKKVEKGYEIIGKSDAIMNVLSMADKVAATE
                     ARVLITGPNGSGKELIARRIHELSNRSKAPFIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKDRRGKFELADGGTLFLDEIGDMSLEAQAKVLRALQENKIVRVGGEKEIKVNVRVL
                     AATNKDMKSEIAANRFRQDLYHRLSVIVIEVPSLNERKDDIPLLAEHFNQLICNEYGR
                     CPKRITEQAISKLQQINWTGNIREFRNIMERLIIFSDNEIDENIVELYAKPIFKE"
     gene            complement(76690..77211)
                     /locus_tag="PK252_02815"
     CDS             complement(76690..77211)
                     /locus_tag="PK252_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13671.1"
                     /translation="MAKRFISSKLGKIFIISLLLITYIGCDTDTTPVVPEVPVYIELS
                     LTSDLAYLGTDMVVSVYPDSINENYTIIDYHNPKFPKKRITYKTYGNGVLIYQRGIDD
                     FQAFDATCTYRAYDDYCKLSIDSAGWKGVCPCCGSEFMLITDGAPTNISKAYKPLMQY
                     QTTLRSNYLIISK"
BASE COUNT        23611 a        13992 c        15419 g        24406 t
ORIGIN      
        1 caaatttgtt taatttttaa taacgtcccg attttaatgg gacactagtg ttgtaacgtt
       61 tttaataaat tggttgtgaa gcgtttaata ttttttacag gggttgtagg gcttacattt
      121 tcagtatttg gtcaaacaaa agattcattg aaatatcaat cagatagtat tgatatcagg
      181 tatttgtaca aaattaacga tacgctgagt tatcgttatt atccaacacg caggtttgga
      241 tttgtaagta atgcttttaa agatttttat ctgttgccga cagaattggt taagaaggaa
      301 aatttcaaag gattggggat aattgctgct tctacggcta ttttaattta ttatgaccag
      361 gaactggttg atgctgccca acgatttggg aggtatatca atttgtcggg agaaaataat
      421 gtttataatc tgacaggtac aaaggatttg cgtataaatg tacctacaga tttatcgtct
      481 ggtttgtact acattggtga tgggataaca gaaatgggag tttgtgccgg tttctatata
      541 tattcgtcat ttacagatga taaacgtgct gcccaaaccg ctgcccagct tgctgaaggc
      601 atgcttgccg taggggcatg gattcaaata ttgaagcacc tgacagggca cgagtctcct
      661 caatgtgcaa cagtttctgg tggaagatgg cgttttttcc caaatccggt tgactatcaa
      721 aataatgttc ccaaatatga tgcatatcca tcagggcacc ttgctactgc tatgatgaca
      781 gctacggtag tatcgatgaa ttaccccgaa tataaattta tcaagccgct atgttatact
      841 ttgatggggc tttgcggtta ccagatgatg aacaacggtg tgcattgggc aggagattat
      901 ccgcttggca ttgccattgg ttacgcggtg gggcgtatgg ccgtgaacag gggcagggag
      961 gaagtgaagc agcctttaac atcaatcaga gagcctaaag cctcatttca gcttcacccg
     1021 gtgtatatgc ctgaaggggc tacaggcttg gctttgcagg ttaagtttta atgaagaagt
     1081 ttacggaaga gtaaagaagg ataaagaaga gtgaggaagc gaacggaaga ttagagaacg
     1141 gtaaagaaga ttgaagaaag ataggggaga tgagtaggtt tgtagtattt ctttctttgt
     1201 aaatactggt tggcttatat tttgtaaggg ttataaatta aaagtcagat aaatatgatt
     1261 actcccgaac aaacgtacca gaagttcgct gttttctatg atttatatgt gggaaggttt
     1321 agtgcagatg tagatttctg ccattcatac tgtagtagcg ataaaagcat tgtagaaatt
     1381 ggctgtggaa ctggtcggat cttaaaatac ctgctggata agggattgtc tgttactgga
     1441 attgatattt cggatgaaat gttgcaaaag gcaggagcca agcttgaacc gtttgtttcg
     1501 tcaggacatt tgaaactttt gaaccacaat tttgctgaaa aaccttttga aacgaagttt
     1561 gatgttgcgt tgttgacttt ttatactttc aattatatat tggacaagcc tgaggccttt
     1621 ttggccaata tacaccagtc gcttagcaga caggcggcct tgttgataga tgtgttttat
     1681 ccaatgccat taaaaaataa ggccattgac aatgtatgga ctgaaaaatc tttcgaaatt
     1741 gaaggacagc aggtgctttt aaaagacaag cgaacaatga acggtaatat tgagcatcgt
     1801 tgccaggtgt ttagcataaa tggtagcgaa ggaatatcta ttgacacttt taggcgttat
     1861 tttccgccaa aagaaatggt ttatttattg aaaaatgccg atttttctga tgttaaactt
     1921 gccccgggct atgacttcag aagcctgaaa tgcgaattgg aggagagtga gttgggtgat
     1981 aattttattg tggtggcaaa aaagtaattt ttttttactt ttagggttat aaaaatcctt
     2041 caccatgaaa aaaatagtat ttttattcag tattgcttct tttttgttgg taaatattgc
     2101 agccaaccct cttccaggaa cagatataaa aatcaaggct ttttattttg atgacaacga
     2161 taattgggtt ttaaagcttg atgcctattc attggattat tacaatattt tagacattga
     2221 cactgtttgg gtgtgctcat caactgaatg tgccaggata aataagactt ttgttgactt
     2281 taataattta gatattatta ctgaggatag tttagataga cctttgaagt ttaataaaga
     2341 aggggattct ataagggtta agttttacta taaacatttt agaaatccag gttcagattc
     2401 agtatttata gagacccttc ctgattggta tggagcatca ttaacttatg gcaattacgg
     2461 taattcaaat gtcccaagat taagcaaagg acaatcaatt gctagtgtgt catattatgg
     2521 atatgattat gtttattatt ctttgagcaa cactataaca gattttaatg atacaactaa
     2581 aatgtttggt gttttgaaag gtcatatcta tgataaaaat ggccgtcctt taaataaagg
     2641 acaatttaca attgattttg gtagaatttt aaaggtgcaa tcgggcttat attcagtgaa
     2701 aatgtattcg aggaaatata aattaaacag cgcctattta ccttctaatt ataattctag
     2761 tgtacatttt aaaccaatag aatttgaaat actacctgat tcgactaccg agcgagatat
     2821 atacttaact gattatgttg gagtaaaatc aatatctaac aaagaaaacc tggttaaaat
     2881 atttccaaat cctgtaaagg gtaattctat attgcaatat gcaatagctc ttccagtttt
     2941 ttcttctaat tgccaactga atataataaa cattaatggg caaagcgtct ttcaatgtaa
     3001 gataatggaa aataaaggga acatacaatt accacaagat gtatttgacg gcacatatac
     3061 tgttcaggta attatgaatg gagctgtttt acattcttct cagctcatag ttgtgaaatg
     3121 aaaaggggca cattcttcat cttatttggt ttattctgga ttcacctgtt ttctcagaat
     3181 aatgtacagt ttaaagctga gtatattgac tttaagttgg atagtaccca atttgccatt
     3241 aacggaatat atgagtttaa gaataactca gcaaaagatg ttgaaatccc aattgttttc
     3301 ccatttgcag aagatgtaaa agatattgat tccattagat taataagcct taacaatatg
     3361 agcgctttgc cttatgaaaa aataaaaaaa gagataaggt ttcgcttgca tatcccggca
     3421 aaagattcat tgagcgtaaa tatttattat agctcaaggt cgaagaaaaa gaatgtttat
     3481 atattgacca ccactcggtt ttgggaaaaa ccattagaaa tagcatacta ttcccttgct
     3541 attgataagg gtattaattt aaaaaccttg tcattgaagc cagattcatc ttctgttaag
     3601 tatggggaac aggtttactt ttggaaaaaa gaaaaatttc ttccagaaaa agattttgtt
     3661 gttgaaatag aatagccatt atttctcgtt acaatatttc cccatgatgt tgtaagcaga
     3721 tttgatgccg agttcgccgg ccttgcttat gtctgagcag ccttcttcct tcttgttggt
     3781 aaagatgtaa agcaaaccac ggttgaaata agcttgggca aagttggggt cgatggctat
     3841 gacctttgaa tagtaatata tggctttgtc aaaggtttta gtttctacca atacgttggc
     3901 caggttgaag taggtatata taaaagaaga atcgtgctgg attgaggttt cgaggtcgct
     3961 gataacctga gtaaaatcag ggtttccttt tacagaggat ggcaatttgt tgccattttc
     4021 gccaatgaag aaaaagtcgc tttctttaga gcttacgttt tgcatcagca taatgtattc
     4081 aaacaagaca taagacctta agaaatacgc aactgcccag tcgggcttga gttttatgac
     4141 caggtcacag tccgagagtg cgccattgta atcttgcaat tgagctttaa ttacacttcg
     4201 gcttaacaag cgtgttgcat tattctcatt ttccgaaagg ttttcaaatt cggcaagcca
     4261 tgagtttaac tggtcataaa tattgtcaat tgcggtgttt gatacaatga gcctttcttc
     4321 tccaaggatt tggttgttta ttttttctaa aacatttgtg tttgtgtact ttttgttttc
     4381 aaagtcggtt tttgaaatac aaacaatgcc gtaggccggg gctggagtta tacctgccag
     4441 tgaattggag ctttctttta cattgcctcg ctcgaaatcg gctttaaagt caatgatttt
     4501 ggccaggttg gtagagtcga gtttttcttc aaagccgctt tcattgcgtt ctttgtttat
     4561 cctggcagcg gttttgtagt ctttttcggc attgtacata tcgcctattg ccctaagggc
     4621 tatagaacgg ttgtagtagg ctgctacaaa gttggagtta aggttaatgg ctgttgtata
     4681 atcgctgatg gcttttttat atttttttaa ggtatgataa acaatgccgc ggttgaaata
     4741 ggtgtaaatg ttgttagggt ttatttctat cactttatcg tagtcgtcca gcgcaccatt
     4801 gatatctttg aagcgtactc taagttcagc ccttacaaaa taagccaagg cattttcggg
     4861 gtcgagttta agtaccctgt taaggtcaat caaagccccc agtgagtctt tcagttcatc
     4921 gcgggtaagg gcgcgcgagt agtaggctgg tgtgtgtgta gagtcaagct tgatggtttg
     4981 gttgaaatca gcgagagctt cattgtattt tttttcttcc agatagttgc ggccacggcg
     5041 gaaataattc tcggcatcga aagggttgag ctgtattgct ttgtcaaagt cgcacaaagc
     5101 catgtaatag tcttttttgg cctggtaggc cattcccctg taaaggaaag cgttgtcgtt
     5161 attcttattg agcgtgatag caatgttaaa gtcctggata gcttcatcgt ttagtccaag
     5221 catcaacccg ttcaatcctc tcctaaggta ggcttcggta tttgtggctt tgagcattac
     5281 tgctttgttg aagtcttcgc gtgcaccgcg cacgttgtac atgcgtgact ttatttcacc
     5341 cctgaggatg taatagtatg agttgccgcc cttgattttg atggcagtat ttatatcttg
     5401 caaggcccct acgtagtctt tcaggctgta ttttgagtat gccctgaggt aataagcttc
     5461 atcgaggtca gggataaatt ttatcagctt gctgaaatat tctatggcta cagtattgtc
     5521 tttatctatt tgttctcttc cggcattgag ccaatggttg atgtttaact gggcatggat
     5581 atttcctgcg aaactcagtg taaataaggt aaatactatt ttgtgcaata tatttttcaa
     5641 tgataacaaa ggttttattc taatacaaat atatcttcat tgtcgcgttt cataaaatat
     5701 tgctcgcggg caaatttttc gaggttgtcc ctgtcggtac gcaattcgtt gagtttatga
     5761 ctgtcaattt ttattttttc aatgtaaaat tctttttctt ctttcatttc ataatatttt
     5821 tttagggcag caacacggtc aatgaggttg ttttggtcaa agaaaatcaa ccacaggaca
     5881 aagaataaag aggcaagaaa atatttgttt tttaaaaatt ttaaaacctt gttgttgttt
     5941 ttgctcataa aaaatataac acttaacagg cacgaaggta atattttgcc agagaagaat
     6001 gcattatctt tgggcaaaaa ataattaatg tttgttaatg gacaaggaca ggctgatatt
     6061 gattaccaac gatgatggga tacatgcaaa aggcatagaa gcccttgtta atgtggctaa
     6121 aagttttggc aatgttgtgg tggttgcccc gacagaggga caaagtggta tgtcgcatgc
     6181 tattaccgta aaaacgccta tcagggttta taaacatggc aaaaatggca acgtagaaaa
     6241 gttttcgtgc aatggtacgc ctgtggactg tgtaaaaatg gccatgagca aaattttgcc
     6301 caaagaaccc gatttgattt tgtcgggtat aaaccacggg ggcaattctt catcgagtgt
     6361 gctgtattcg ggtactatgg cagctgcttt agaagggtgt ttgtatggga taccttctgt
     6421 tggtttttct ttgcttgagt tttctaagga tgctgatttt tctgcagcta ctttttttgt
     6481 caaccgcatt attgacaata ttttgaggcg gggcttgccc aaaggagtat gcctgaatgt
     6541 caatatcccg tatttaaagg ccgaacagat aaagggagtc atggtatgcc gccagaatca
     6601 tggtatttgg cgcgaagaat ttgaacatcg cactgaccct gccggacacg actattattg
     6661 gcttaccggg acatttgaag accttgagcc tcatgcaaca gacaccgatg aatgggcttt
     6721 gcgcaaccaa tatattgcta taacccctgt acagtttgat tttactgcac ataaagcttt
     6781 gcacacactt aaacaatgga attggaatga tggaacaact gattgaaaaa ataaaagcta
     6841 aagtggccag gtttgacaag ctttatgtcg gcataatatt gggattgtta gttccagtgg
     6901 tggccctaat gggtacgtat ttttatacgt ttaccaatta tacgttaaac cagttttttt
     6961 attttcttat tatgctaagg gtaataacca agctgtttag cttgtgtatt atccccaacc
     7021 tggctgtttt ctttatcttt ttatggttag atatgaacaa ggctgccaaa ggtgtgctga
     7081 ctattacttt tatcatgaca gtcctgattt tagcttatca agcccacatg ggtttgctgt
     7141 ggtaaataaa gtactgtcat tcctttggaa atgaaaaaag cttaactttg gactaaaata
     7201 ctaccataca tggtttttct taaccttatc ctggagagcc tgcaaatggc ctggcactct
     7261 atcagtcgca ataaagtacg tacatcgctt acgctgcttg gtattactat aggtatattt
     7321 gctataatct ctgtttttac tgctcttgat gcgcttgaaa atggcattcg tgacaatgtt
     7381 gcgagccttg gcgatgatat tatttatgta caaaagtggc cctggagctt tgatgatgac
     7441 tacaagtggt gggaatatat gaaaaggcct gttcctcgtg ttgctgatta tgaagaggtt
     7501 ttaaaaaagg cacaaactgt tgatgccgct gtgttttcag taagtactgg ccgaactata
     7561 aagtacaaaa acaattcgtt tgggaatggc ggtatttggg ccaatactga tgattttgaa
     7621 aaggtaaaag ctttcgaaat tgaaaaaggg agatactttt cgccttacga atttcagtca
     7681 gggaaaaatg tttgtattat tggcaatacc atagcaaccc agttgtttgg caatgaagac
     7741 ccggttgata aatttattaa agtggatggc cgaaaaataa aggtggttgg cgtaactaaa
     7801 aaagaaggaa ccagcattgt tggcgggagc atggatgatg tagtgttaat tccattaaat
     7861 tatgcccgaa atattttcga tatcaaaagt gacatgttga acccgtttat gatgataaaa
     7921 gctaaaaagg gtgttccgct tgatcaaatg aaagatgaac tgcgaagtat tttgagaaaa
     7981 aaacataacc ttaagccttt gcagcccgat gatttttcgc taaaccaagc aagccttatt
     8041 atttcgggga ttgaaaagat ttttttgata attaatattg ctggttggat tattgggggc
     8101 ttttctatca ttgttggagg ctttggtatt gctaatatta tgtttgtaac agttcgtgaa
     8161 cgcaccaacc agattggtat tcaaaaagct ctgggtgcca aaaggttctt tattcttatc
     8221 gaatttttgt ttgaagcggt aatattgtct gttttaggcg gtttactggg gctgtttttt
     8281 gtatttcttg cttcggtagc ggcgagcaaa gcgcttgatt ttgaaatgac tttaaccatt
     8341 ggcaatatat tattgggtat tttgacatct gctattattg gccttttggc agggcttata
     8401 cctgcatata ctgctgctag actcaatcct gtagatgcta taaatactac tttttgatgc
     8461 aaaaacaaac ggcattgccc tttttcccta cttctgccaa agaagttgaa cagcttggat
     8521 ggacaagcat tgatgttatt ctttttacgg gcgatgcata cgttgaccat ccctcgtttg
     8581 gtgtggctgt aattggccgt gtgctcgaaa aacaagggct taaagtggcc attgtgcctc
     8641 agcctaattg gcaggatgac ctgagggatt ttcgtaaact gggcaaaccc aaccttttct
     8701 ttggcatcac tgccggcaac atggactcga tgataaacca ttacacagct gccaagcgcc
     8761 tgcgtagcaa tgatgattat actgccggag gaaaggctgg ccgtcgtccg gattacgctg
     8821 taaaggtata tacatgcatt gtaaagaagc tatttcctga tgtccctgta atacttggag
     8881 gtatagaggc ttcgcttcgt cggtttacgc attacgacta ttggtcagat agtcttatgc
     8941 cttccgtatt ggccgaaacc aaggctgatt tgctttttta tgggatggca gaaaaggctt
     9001 tggaagaatt tgtcaagctt ttgcaggctg gtaagccttt ttctgaagcc aggaaaattc
     9061 cacagttggc atatctggtt aaagataagg ctgatattga ggttgaagat ggcgcgaaaa
     9121 caatatatct ccatagtttt gagaaatgcc aaaaagataa gttggcattt ggcgaaaact
     9181 ttaagtttat agaagaagaa tcaaataaga tacacgctgc tcgcattgtt gaacctgttg
     9241 gcaatggttt tgttgttgta aatccgccct ttgcgtattt gacacaagat gaaaatgatg
     9301 ctccgtatga gctgccatat tcgcgtttgc cacaccccag gtataaagaa gctattccgg
     9361 cttttgatat gataaaaaac tctatcaata ttcatcgtgg ttgttttgga ggttgttcat
     9421 tctgcaccat ttctgcccac cagggaaagt ttatccgcag tcgttcgcca gagagtgtga
     9481 tgcgcgaagt gctcaatttg tctaagatgc catatttcaa tggtactatt accgacttag
     9541 gcgggccctc ggccaatatg tacatgatga gcggaataga taaagctatt tgcgaaaagt
     9601 gtaagcgtcc gtcatgtata tttcctgcta tctgcaaaaa ccttgatacg aaccataagc
     9661 cgcaaaacga tttgctgcaa gctgcaaggg atgttgctgg tataaagcac gtttttattg
     9721 ggagtggtat tcgttacgac ttgcttttca atgctccaaa cgttgaaaca aagaaatcat
     9781 ctgatgagta ttttgctaat cttgtgaaat atcatgttag cggcaggttg aaagtagccc
     9841 ccgaacattc gtctgacgag gtgctcaaat tgatgcgcaa accttctttc gcccttttta
     9901 aacgtttcaa agatgagttt gacaagctaa atgccagatt tggcaagcga caatacatta
     9961 taccttattt tatctcaagt catccgggct ctaccattga cgcaatggca gcattggccg
    10021 agaataatag gcaattgaac attaatcctg agcaagtaca agattttacg cctactccca
    10081 tgaccttatc ttcttgtatt tactacacgg gcattgatcc ttataccggt aaaaaagttt
    10141 atgttgcccg ctctaaagac gacaaaaaaa tgcaaaatgc cttctttttt tggtataagc
    10201 ccgaatatcg aaaaatactt tttgaatacc tgaaaagaat aaaacgtacc gacattctta
    10261 acaagctata cccaagacgt taacaaaaaa ttagaagttg catttctgat tgccattatc
    10321 tttgcaaagc cagttaacgt aaattaatta aatttcaata tatgaaaatc aaactactga
    10381 ccatcgttgc aataagctgt tttgtagcat ctttgacatc gcaaactatg aatatcagga
    10441 taaaccagtt agggtattat ccacaatctt caaaagttgc attggttgtg tggccaatgg
    10501 gtaatgaatt tgaagttttg cagttgcccg atagttctgt tgtttattca ggaaatttaa
    10561 gtgctaaaaa gtattataat gactcaaaag attctatttc tatagctgat tttagcagtg
    10621 tttcacaacc ggggcaatat tgcctcaggg tgaaaggtat ggcggtttcg ttgccttttg
    10681 ttatatctga taatgttttt cgtgaggtgg cttatgcttc attgcgtagt ttctattttc
    10741 aaagatgttc ttatgctttg gatgaaaaat atgccggaat atggaaacgt aaagccggac
    10801 atcctgatac atcttgtgtt tatcacgctt cgacagggcg ttctggtaaa gtttcttctc
    10861 ccaaaggttg gtacgatgcc ggagattatg gcaagtatgt ggtaaatgcc gggatttctg
    10921 taggagatat gcttcagttt tacgaattgt tcaacaccta ttttccggac tcatctgcaa
    10981 gtatccccga atctggcaat ggggttaatg acctgcttga cgaagtgcgg tttgaactgg
    11041 actggctggc tactatgcag gataccgatg gcggggttta tcacaagata acaacagcgg
    11101 ggttccctgg ttttattatg cctgaagatg ataaagatat ccgttatatt ttagggaagt
    11161 gtacctctgc ctcgcttgat tttgcggcta tgatggctat ggcaggccgg atttataaaa
    11221 aagcagattc tgcttttgct gccaattgtc ttgctcaggc caagaaagca tgggattggg
    11281 ctgtgcttca tccgagtgta gcatttacaa atcctgttgg aattagtacc ggggggtatg
    11341 gagacaacaa ctttagtgat gagtttttgt gggctgcagc agagttgctc atttctaccg
    11401 gcgatagcgt ttattacaaa taccttgttt ctaaaaaatc tgctttagtg gcaagtgttt
    11461 cgggatgggg ttcagtagga gggcttgcgg cccaatcgct tgctgttatg ccttcaatac
    11521 ttgatactgc tattgtttca aaaattaaaa cctcaattat aagtgtggcc aattctcata
    11581 aaagcaatat tcaaggaagt gcagccaggg tttcaaatac tggttattac tggggttgta
    11641 acggagctgt atctcaaaca ggcattctgc ttatttatgg ttatttgtta agtggcgaca
    11701 gtagcctgtt gaatgcttcc attgaacttg ccgattatca gttggggaaa aatgccacag
    11761 gctattcttt tatcagttat tatggcaaaa aactggtgat gaaccaacac catcgtcctt
    11821 caattgcaga tggcgttgtt atgcccgaac ctggtttcat ttcaggaggc cccagtgcag
    11881 gaaaagaaga taacaaaaac atgtatgctt ataccactaa tgctcagtct tttatagata
    11941 acaccagttc gtatgcatca aatgaggtgg ctataaactg gaatgcttcg cttacggcac
    12001 atttggctgc tattgatgca attttgggca ataaagatgc ttttgtaatg cctgttgtta
    12061 gttctgttgt caaccctgct aacctgactt tgactaagcc tgctcataat gcaagttttt
    12121 atgtcggaga ttctattgaa gtgaaggtta ctgccaatgc agctgaaatt caaaaaatag
    12181 aattttactt aaacaataaa tatctgaaaa cggtttatga tgctgcttcg ccattcaaac
    12241 ttgttttgcc tgttggtaca tataccgtta cagcatgttt aattgataaa aacgggttgg
    12301 ttacagaaaa agcagctttg attactgtca agcccaaaac aggagttgat gctattaaaa
    12361 atgaaagtcg gttgaaagtc tttccaaatc ctggttcgaa aaaaattact gtccaataca
    12421 atgcctcatt atttggcaaa gtagttttac aattgtgcga tatctcagga aaaaaaacag
    12481 tattatggac cggtcagatg aaaaacgaaa agaacacact tgatattaca atacctgcct
    12541 cgcttaccgc aggtgtttat atgttagaag ttgttgaagg ggcaaaagtt atagaaagca
    12601 ataaggtagt tttgcaataa tttttttgtt gcaacctgtt ttatcttatc ctgatgcgta
    12661 ttatacttcc tggtatcaag ctttgctcgc tgtgcaagtt gttgatgagc ataatgtcgt
    12721 taatagagtc gcatttgtat cgttgagcaa tgccccaaat ggtatcacct ttttttacag
    12781 tatattcgaa gtagttatct ttcttattct ttgtattatc ttttttccag atgataagtt
    12841 gttgtccgac cttcaggttt tgagatttga gcttgttcca tatcataata ttgtcggctg
    12901 tacagccatt ggcaagggct attttgtgaa gaaaatcacc tttttttact tcataaaagg
    12961 tttttaccat cccttgggta tttgacgaag cttccatttt ctggaacata tcaagcgtat
    13021 cattagtttg gctgtatatt tggttttctc gttttaaaaa ggccaatact ttgtcagaag
    13081 gtaaaacaag agtcaagccc ttttcacgca tcgggatata gtcgcgtctg tagcaggggt
    13141 tgtatttgcg gatagagtca atgctcattc caattttttc ggatagttta tcaaactcta
    13201 atgcctgcat tatctgcaag gtgtctgttg aaatgcttcc gaagcctgtt tttgcagcat
    13261 atataccatg atcggcagct tggttatata aatatactat ggcaataaaa gccgggacat
    13321 accactttgt ttgttcgggc aaatatggtt gtatttccca gaaagtagtt ttgcctcccg
    13381 agcgttctat ggctttttta atttcaccag gcccgccatt gtaggcagca agggcaagtt
    13441 gccaatcgtt gaatacagaa tacagatatt ttaaatatct gcaagcagca tctgttgagg
    13501 cataaacatc gcatcgttgg tcaacataag agctaacttc caaaccaagc agttttgaag
    13561 catctgccaa taattgccat aagcctaaag ctccagaaga agaacgggcg gcaggatcga
    13621 ggtttgactc taaaatggca agatatttca gttcgaaagg cagttggtat ttatccaaat
    13681 agcggtcaaa gatgggaaaa taatattggc tcagttcaag aaattcggct attttctctt
    13741 tacgttgggt aaggtagagg tcaatatatt tctggacatc agcattgtaa tcgagcttga
    13801 tggccgaatt gtggttgagc aattcaattt tcatctcgta gtacatagga ggtagcgctt
    13861 tgacctcgct attgtttttg ttggcttttt tttcttgtcc ggatattgaa gggtaagtaa
    13921 aggcagtgaa taacaacaaa aacaatgtta ttaaattata ttcaaagcta atgcgatggt
    13981 gcatagtttt ttaattcctg aaattactgt tttttttcat caggggtatt attagtctca
    14041 cctttgctgg cttttttaaa ttcgtgcatg ccttggccca ggccacgcat tagttcaggt
    14101 atttttcttc ctccaaatat cactaataca acggccaaaa tgagtataat ttctgttgtt
    14161 ccaatagcgc caagcattac atgtaagttt tccatgactt ttattttaat gtgtaaataa
    14221 tcgtataatg tcgttcaggt ttgcatagat gataagcgaa aataagatga caataccagt
    14281 aatttgggca tattcaagga atttgtcaga aggcttgcgc cctgtaacca tctcgtaaag
    14341 gaggaacagt acatgtccac catcgagggc aggtataggc aatatgttca taaaggccaa
    14401 tacaatagac agcagtgctg tcagcgccca gaatgattcc cagtcccata ctcctgaaaa
    14461 taaattgccc atggtgataa agccacccaa agattcgtat cctttggttt ctttagaaaa
    14521 tatcaactta aactgtttga cataattgct taatgtgttg aagcccatac tgataccagc
    14581 aggaatagag gcaaaaaaac catagtcatt atgctttagc tggaaatatt tagaaatatt
    14641 tccatcaagt actacaccaa gtagtcctgt ttcgggtaca gtaatgtcaa tttttttcaa
    14701 cactccattt tgctcaatgg taagggtaat atttttgttt ttattggctt gaacaatggg
    14761 cttaatctga tctacgaaaa tcacttgcga gtcgttaaca gccactacct tatctccggg
    14821 ttttaagcct gctttttcgg cagcggtatt ttttactaca tcacctacaa tataaggaat
    14881 gcgaactgtg aaaaagtctt ttttcctaat caaagtatag ttaaggtttt ctggtaaagc
    14941 aatgtttatc aacttaccat ctctgatgac ttgtaccgtt tttgcctctt caaggacaat
    15001 agtagggact attttaaaaa agttttctac ttctttatag tcaaccgtaa gaattttgtc
    15061 gccattgcga aaaccggcat tgtatgctac tgaatctact gctataccat aagttacatt
    15121 tttagtaggc aggtattctt cgccccaggt gaaaagtatc cctacgtata ttgccaaagc
    15181 taataaaaag ttgactgtaa caccgccaag cataatcaaa agcctttgcc atgttggttt
    15241 cgacctgaat tcatacggtt gggcgggttg tttcatttgt tctaaatcca agctctcatc
    15301 tatcatacct gatattttta catatccgcc aagtgggagc catcctatac catactcagt
    15361 ttcgcctttt ttatatttaa aaatagaaaa ccaggggtcg aagaaaagat agaacttttc
    15421 tactcgggtt ttgaaaagtt tggcaaaaaa gaaatggcca aattcatgta aaagtacaag
    15481 taatgataag ctgagcaaaa attgtaaggc tttgataagt atttccatgg atttgtttta
    15541 tggtttttaa tttaaataag ttgcaatgca agttctcgtg caaatttatc tgtttgctga
    15601 taatcttcga tgcctgaatc tttaataaat gttatttttg ataaagtttt ttcaattaag
    15661 tcggacatgc ccagaaatcc tatatgtcca cacaaaaatg cttcaactgc aatttcgtta
    15721 gctgcattca ttgcacaagg catgtttcca cctttgtcca tagcatctaa agcaatagcc
    15781 aggtttctga atactgtcaa atctggtttt tcaaatgtta aagccgggta atctgcaaaa
    15841 ttcattcgtg gccaactggc tttcaatcgt tcggggtaag agagtgcgta ttgtattggc
    15901 aaacgcatat cgggcaatcc catttgagct ttgatagagc catcttcaaa ctgcaccata
    15961 gaatgtatga tagattgggg atgcactaca acttctattt gtgagggttg caaaccaaag
    16021 agccaacggg cttcaatagc ttcaagcccc ttgttcatca gcgatgcaga atcgattgta
    16081 actttgttgc ccatacacca gttaggatgg ttcaaggctt gttcgggggt aacgtttttg
    16141 aggaagttga tatcttttcc acgaaaaggc ccgccagatg cagtaagtag tattttttca
    16201 accggattgt gaaattctcc tgtaagacat tggaatatgg cagagtgctc tgagtccacc
    16261 ggaaggatat ttacccgttt ttctatggct aattttgaaa tatattctcc agcaacaaca
    16321 agtgtttctt tgttagccaa agctatggtt tttcctgcat ttatggcatt aatggtagga
    16381 gttaagcctg aatatcctac catagctgta agtactatgt ctattgatga aaattgtact
    16441 acctcagcta ttgctttttc tccagcaaat acttttatcg ggtaattggc cagagcttct
    16501 tttacaaact ggtatttttc ttcattggct ataacaacct gattgggagt aaactgcatt
    16561 gcctgtttga taagcaattc agcattgttg tttgctgtaa gggtttcaac ttcaaatgag
    16621 tttaagttgt ttgaaataac atctaaagcc tgtgtgccaa tagaacctgt cgatccaaga
    16681 attgctatac gttttttccg cttcattatg agcttgttgt tttagaaaac aatataatct
    16741 acggggttta caggatgacc attgttccat aattcaaagt gcaaatgagg gccagtgctt
    16801 atttctcccg aatttccaac aattgcaatt ggctcgcctg ccacaaccct gttcccactt
    16861 tgtttaagca gttcggcatt gtgtttatac acggtaatta tattattgtc atgttgtatt
    16921 tgaataacat agcctgtttc aagtgtccat gttgccataa taacagtccc gtcaagtgcc
    16981 gctttaataa cagcattggg ctctgaaaca acatctaccc ccaaatggtt gatattagga
    17041 ttaaacccat ttgatataat gcctcgcaat ggagaaaaaa aatgcacttg agctaatgtt
    17101 tgtgtggtat tattgtttga agcctgattt ggaggtgtaa ggttgaatcg ttcttcttct
    17161 tctacttttt ttcgcaataa agagtctttg ggagatttgg taaatatgat ttctttaatt
    17221 tttacagagt ctttggtcat ttctccatac gaaacaggat tattgccttt taggatatca
    17281 ttggcaacag ccaggtacct gtcgcggatt tctagttcat gttcaagcga atcaagcttc
    17341 aatgcattca ttaccatttc tctttttatg gtattgtcag ggtatccggg tatcagttca
    17401 cgaattgaag tgtaagctat tagtataaaa gtaaggctta ttaataaaaa tcccagcgct
    17461 ccgattatcc aaagcaaatt gaaacctttg agcctaacaa aaccaatttc ttcaaaagta
    17521 ttgtcgttgt atataatgag ccggtatttg tcgcgtaacc tatctgatag cttgcgttca
    17581 gatttttgca tatcgtttta ctttagcttt tggaacattg ctgatctggg tggtttactt
    17641 ttacttcttt tggaaggctt tgccatagcc cgtgtttggt acaatatgac atggctgtaa
    17701 ggttcatact tactttgggg atgatagtaa acactacttc aacatggccg ggctgattgc
    17761 ccattgcccc cggaaatatt gtagcttcag caagcaacgt ttcgcggttc cacaattgta
    17821 ccttgccaat aaaatggtcg ccatcatcag gatgggcata ttggctgcca aggaatatcc
    17881 tgacagggaa cctttcattc tcgtttactt ccgaaggaca gtcgataaca ggagtatgtc
    17941 tgtcaaaata atctttttgt acctctttgt caaattgttg aatgtcagta gcagtaaaga
    18001 ttttaggcat atcaatatta ttaagggcat aaagatacaa aatttccttg cgaaaaaatg
    18061 gaactgggat acaaatcaaa attttctgat aataaaagat aacatttgca aaaaattacc
    18121 ctgctcccca aaattatcta actctcatgg cttgctccgc gcttgtgata gagggagcat
    18181 gttaaacgga tattatggct cgtctgaaaa aaagatgcaa aaaaatggca tttattgtga
    18241 cgggcaaaat ttatctaatt atcatttgtt ttgtattaat tgaaagataa agttaccttt
    18301 ggttttttaa aatatagttc tatgagtttg tttgataaaa tcggcgaaga tattaaagct
    18361 gctatgttgg ctaaagaaaa ggaaaagctt gaagcgttaa gggcagtaaa agcggccata
    18421 ttgttggcca aaactgaaaa aggggcatct gacgagttgt cgcctgatgc cgaaatgaag
    18481 ctcttgcaaa ggttggttaa acagcgcaaa gaatcggccg agatatttgc cggacagaac
    18541 aggagcgatt tggctgacaa agagttgttt gaagcttctg ttattgagca gtacctgcca
    18601 aagcaaatga gcgaagagga aattaaagct gcactgaaaa ttatcattga aaaagttgga
    18661 gctaaaactc ctgcagatat gggcaaagtt atgggtgttg ccactaagga acttgctggt
    18721 aaggcagatg gaaaaactat atcttcactt gtcaaggttt tattgggata agcataactt
    18781 gttatagctg aagatttgtt agataattta ttgcttttaa aaaaagttta tatttgcaaa
    18841 caagaacctt ttaaatagaa ttatgaagcc cttaaaatca ggaactgcac ttgcctattg
    18901 gttgctaaga atagcattgt tcattttcct atttactata ttttatagtg aaatagggaa
    18961 ttttaatttt cataaccttg aatcatactt tgccgttgct tttgtagcgt tgggggcttt
    19021 attgtttgtt gggggcttta tggcaaaacc tacattaaca gttatttcag ggatacttat
    19081 caccttgctg tcgtcttatt cattatttgt tcagttcaaa gcaggtttga atgcttcaat
    19141 agccagtttt attgtagtgt ttagcattgg tttttatttt acctgtgtag gaaatcaata
    19201 aaagtatgat aaaaaaagca ctttacataa gctttacttt tttattgtcg gggaatgttg
    19261 cgtgtaattt gtatgcacaa aaaacaaatg aactgcagga agctactgtg gtatataaaa
    19321 agaagccagc tgcgcaatct gcggcagaaa cttcaacttc tgctacctct ggcaatactg
    19381 ctcctgcaga acctcccaaa cctgttgtaa agcttgatac catagtcaga ttaggcggga
    19441 aaaagattat ttgtaacgtc aaaaaggtaa acccaactac ggttgagtat gtaaagcctg
    19501 gcaatcctac gttggtagaa ataaaaagga aagatataga aaaaatcgtt tatagtaatg
    19561 gacgtaagga agtatataac aagccaatat tttcagtgtt agacaaactg caatggcagg
    19621 ccgttctggt taccgaaaat gaggcagaag tctctggttt atataaagtt ggggttgtta
    19681 aagcaaatgc ttcttcgagc agccgcagcc ctaaggcggc taaacaaagc gctattatcc
    19741 gtttacagaa gaaaaccgcc aacatgggag cgttgattgt acttattatc aattctgaga
    19801 tgaaaggtgg atatggcgaa attcccggat gggagattga agggattgct tatagcgata
    19861 cacctcctgt agatacagcc tctgtaaatc aacaaatacg taagctgata gaaaaaaaca
    19921 aacaacgtgt aaacgcttca aaatagtttg tttttcgttt tgtcgtccag ccttttttct
    19981 ttaagtttga taaaaaagtt gcggccatgg actacaatct catcaaagat aaagtgtttt
    20041 ctgccgggtg gtttaaagct tggtcattta ttgtagtagg ttcatttatt ctggcggcag
    20101 gctttgtttt ttttataact ccgtataaga ttgtacctgg gggagtttat ggtatagcta
    20161 ttgttataca ctatcttacc aaagggttgt ttgcttttgc cccggagggc ttgcctattg
    20221 gcacaatggg tttggttatg aacatcccgc ttacctggct tggaattaaa atacttggtc
    20281 cgcgttttgg catcaaaacc atcataggtt ttatcctttc atctgtgttt attgacctta
    20341 ttagttacta ttatggttat gaaccattgg tgaaggacga cgcattgttg tcctcaattt
    20401 ttggcggtgt acttattggc tttggcttgg ggttaatatt taaatcgaag gctacatcgg
    20461 gtggttcgga cattattgcc atgatattgg ccaaatatac ccgtttacca ttggggcaat
    20521 tgcttattta tgttgactca actattgtgt tagttggcct tattgcattt atggattggc
    20581 gaataccgct ttattcttgg attgtaatat atatcaccgg gcgtgttatt gatgggacgc
    20641 tggaaggaat atcttataac aaaactttgc ttatcatttc tgacaagtat gaggatattc
    20701 gtaccaaaat actgtatgac cttaaccggg gaggaacgct cataaaggcc gaagggatgt
    20761 acgaagggaa agaacgaaag ttgattttta ccaatgtttc gaggcgtgaa gcctacattc
    20821 tgaaagaata tatcaaacaa gttgacccag atgcttttgt aactattatc gatgctaatg
    20881 agataatcgg cgatgggttc aaatcgttta aagagtcgtt gtaggctaag ctaatattct
    20941 aagtcttttt cggaaatcat gctatctgcg acaaccacag acctgatatt ggaagcatct
    21001 actacttgag tttcaagaaa aataaccggg atgtcaaatt tcccgttgtt gatggttgtt
    21061 ggtgctattt ttgcaatatc ttctccttgg gcaattttta ttgaaagttc agccactaat
    21121 gcggccatct tttttacaga tttgtaaata gagatagact gcttgttttg aagcatatat
    21181 cggcatgcca aaggttcagc cccctggcct gtaataatga tattttccgg agagtaccct
    21241 ttttgcgtga taatatttat gcaacctctg ctaatattgt cattggccga aagaataaca
    21301 tccgggacac tgatgctata gttaatgtat tttttcatgg tattgtatgc atcatcttca
    21361 gaccagtttt ccatgtatgc ttcatagctt agttttattt ttcctgattt tacgtatgga
    21421 tcaataacct tatgaaaccc ttcgttcatc catacggcat taatatcgct tttatctccc
    21481 gaaaggatta cataattgcc ataaggcttt ttagatatag cataagaagc catttgttcg
    21541 ccaataacat gggcatcaaa ggaaacaaat aagtcgggct ggcaattggc aatcaaccgg
    21601 tcatacgaaa taacttttat ccctttatta tgcagctttc tgaccattcc ggcagcagta
    21661 aacctgttga cagggtctaa tacaacaagg ttaacccctt tttgcaacat ctcgtccagt
    21721 tgtttttctt gcagcaaagc atcgttgtct gcagaataaa acaatatctc gcccccggct
    21781 tcatttattt tttttgtaaa aaggtctctt tcaacttcat accttttgtt aagcatattg
    21841 ggaagcatga agccaatggt tgttttttct tttgtacaag aaattgacag aatcaatgag
    21901 cccaaaataa aggataaaaa gtagtttttc atttcatggc tattttaggt gaaagtatat
    21961 tttattacgg aataaacagg aaaatgttat tgcaaaaaag tggaaataac ggttgatgtt
    22021 gtttcttttt ttatgagcta ttgactttga tgtcaatatt ttataccttt aatgggatta
    22081 agcattttag taaaaactta aaaaaaattg ttatgaaagt agttcgttat cttgcttggg
    22141 cttcagtagg cattgctgca ttaattatgg tgctggcaat aatatcctac ttcaccaaac
    22201 atttactttt aaacgtaggg catggtgcaa catttttaca tgccataaac agttttcttt
    22261 tgctgggcat agcccttttt atagccagca agcaatgctg ttgtgacaaa tgttgcaata
    22321 aggacgaaaa ataagagaaa ttagcgttgc aaaggtattt atgagtagag gcatgtgaat
    22381 ggcatgcctt ttactttttt tcgggatact tcagatgcca cgagtaaagt aatatgccca
    22441 acgggactat cagcacggca agggttgtac cggccagcat caatgccgtt tttaacgaat
    22501 aaaaatcagc cgcccagcca aataaaggcg acatagccga aaagaacaaa cgaataatca
    22561 ggttacgaaa tgataatacc gatgcccgga tatcagaagt aatgcgtttg ttgatactgt
    22621 ctttcagaag gggagtagct attccgcgca ccaggtaaaa cataaacaag atgattaagc
    22681 cccaataggt ttgggttagc gatagggcta tatatgcaac aatagtaaat aagccaatag
    22741 ccaatgttgt gtttttttcg ccccagcgcc tttctatcct gaaagcaaca aaagctgtaa
    22801 tagccacaga aatattaagc aatgtccaca atacaccata cagggccgat ggtaaatgga
    22861 taactttaaa gaatggttgt gcaaaccaag ccatactcaa tgttgccgaa cctataacag
    22921 acgaaaacag catgtaaatc atgagttgtg aaggtttact tacaacatcc ctgaaaacat
    22981 tgacaatact ccgaaaggtt actttatttt ttccttcact cagggtaatt tcttttaagc
    23041 ccaatgcggc cgggatggct ataagagcaa tgcaagcctg cccaatgaaa ggtagccgtg
    23101 ggctgatagc ggccagcaaa cctcctgtta ttcctgcaaa agcttcggca aagttaccta
    23161 atgaagtcat tcggccttca attttcaggt aatcgttttc ttttttatgg gctatcaggg
    23221 tatcgtaaag catggcagta tcagcccctg atataaagct ttgccctata cccagcataa
    23281 tttctgccaa aacaaacact ataaacgctg atgagatgga ataagccaaa aagccaagaa
    23341 agccaaataa agccccaata actatggtgt ttttacgtcc ccacctgtct gctgcatatc
    23401 cactggggat ttcgagcagt acgatggtca gcgaataggc tgcctgggca ataaacaggt
    23461 gcctgaccga taaatgactg gcgttgtaaa aaagcagtat caccggcatg gtaagcatga
    23521 accagtgagc tatttttatt acatacaacc tgttgatatt ggcttgcata ttctttcctg
    23581 acaagacctt attgtttttt taaacctgta aagttttaaa acattaatta ttaatagata
    23641 aaaagcattc taatgtaaaa tcctttagag acttgagttt taaatcagct ttatcaaatc
    23701 ctttgtcatt aaacaacacc ggatcgggta tagctacaac cttcatcccg gcagctattg
    23761 cagcactaac tccatgaata gagtcttcaa tgacaaggca ttgtgaaggg tgatagttca
    23821 tcatacgggc agtggaaaga aataccgcag ggtcgggttt gccttttgct tccagttgtg
    23881 ccgagtgata aagctcaaag tattggcgca aatcgagctt gtctaatact gtttctatca
    23941 cttttatagc tgacgaagaa gctaatgcca tgcgtatgcc ttgttttttt agcatttgca
    24001 taacatctac aacacccgtc atagctgttc ctttagcttt aattttttcg ataatgctaa
    24061 attcaatttt agaaatgact tcctctatcg aaaagccttt ccacgggagc ttttcatacc
    24121 aataatcaac cgctcctttg gtggtcatgc ccatcgtctg acggcacata tcttcggtaa
    24181 gtttgatgcc aatttctcca aagacttcaa taagagtctc acgccacaat ggctcagagt
    24241 caataatcac cccgtccata tcaaaaataa cacacgccgt catagaaaca ttttaggatg
    24301 ccaaaagtag cgaattttag gcaaaggata gttttttcgc aatattgttg aaatttttgt
    24361 caactcattt gtttatttgt agttgtttat tataaagttg tgagcaatcc taaaagaagg
    24421 ccgaacagac aaaatcggtt gacaaataac tgacccaaac ggagaataag aaaaatggaa
    24481 attgtaaatt tgcttagagc aaaaaaataa tatacaagat taatgacaaa caaaatagca
    24541 aaaccgtttt taaagtgggc aggtggtaaa acacagctca taaatgacat tgaaagagct
    24601 ttgccaaaag atatttacac taataaattc acctatattg aaccattttt aggaagtggt
    24661 gcagttttat tttggatgtt aaacaacttt ccaaacctaa aaaaagcggt tattaacgat
    24721 attaatgatg acttaataaa cacttacaag ataatagctt tcaaacctaa tgaattgatt
    24781 tcaattctcg aaattctaca gaatgagttt cacggacttg aaggacaaga cgaagcgaaa
    24841 aaaaaatact actactcaaa aagagacctt tacaatacga gaaaagaaga acaaacagga
    24901 caagctgctt tgtttatttt tcttaatcgg acttgcttca atgggcttta tcgggtaaac
    24961 agaaaaaatg aatttaacgt gccaatgggc agttacaagc gaccaactat ttgcgataaa
    25021 gaaaatattt tggcagttag taaagcatta caaaaggttg aaattctttg tggtgatttt
    25081 gaagaaacat tgaattatgc tgacaaaaac acgttatttt atttcgaccc gccatataag
    25141 cctttaagcg aaacctcaag ttttaactct tattcaagag acgagttcaa cgatgctgaa
    25201 caaatcaggc taagggattt ttgctacaaa cttgacacct taggacacac atggatttta
    25261 agcaattcag atgttaaagg taaagatata aaagacaatt tctttgacaa tttatattca
    25321 gacttcaata tacaaagagt atatgcaaaa cgaagtataa atgccaaccc agaaaacttt
    25381 tatttgggcg ttcccctatc gggacgggct atccgttcca agtcctcgct acgctgcggg
    25441 ctttccacta ctatccctaa cgcaacttat tgccattaat gaacttatcg tttaatataa
    25501 aacttgcgga aggttaccaa agtaattctc aaatagcaag agttctaaca gaaaattggg
    25561 ttcttacaaa ttcttattgt ccaagctgtg gagatttacc tctaagtggt tttcaaaata
    25621 atcgaccagt tgcagatttt tattgcaaga aatgttcaga agagtttgag ttaaaaagca
    25681 aaagtggaaa acttacaaat acaattacag atggtgcata ttctacaatg attgaaagaa
    25741 taagttctga aaacaatcca aattttttct ttctaactta ttctaataat tggactgtca
    25801 atgatttttt gattattcct aagcagtttt ttacggaaga aataataatc aaaagacagc
    25861 cattagctcc aaccgcaaga agaaccggat ggattggatg caatattgat atttctaaag
    25921 ttgctgaatc aggaaaagta tttttagtaa aaaattctaa agtaattgac caagaagttg
    25981 ttaagcaatc ttttaataaa actttatttt taagacaaca atcaagtgat gccaagggat
    26041 ggattttgga tattatgaaa tgtgtcgatg aaattaaaac aaatgaattt aatcttgatg
    26101 atgtttatgc ttttgaaaaa aagcttaaat taaaataccc tgacaataat ttcataaaag
    26161 ataaaatcag acagcaactc caattattgc gtgataaagg aatgattgag tttgttggtc
    26221 gtggtaaata cagaaagatt caatatggaa acatttaaaa ttgaaattca agagttcttg
    26281 tcaagaataa ttgaagtaga agcaaattca aaagatgaag caattgcaaa agttcgtgaa
    26341 ttgtacagtc ttgaagaaat catcttggac agcgaagatt atgttacaac agaaattaac
    26401 taatatttag aataaactat ctcctcaaag gagatgcgtt gaaactattg cctcaatttg
    26461 accataagtt tgatatggtt tttgccgacc ccccctattt tttatccaac aatggactta
    26521 ctatacaaaa cgggcaaatt gtaagcgtta ataaaggaaa ttgggacaaa tctcacggct
    26581 ttgatcattt aaatgagttt aatcgcacat ggctttcatt ggttcgcgac aaaatgaaag
    26641 atgacgccac tatttggatc agtggaacaa tgcacaatat tttttcggtt ggacaaatct
    26701 tgacagaatt gggctttaag attttgaata taattacttg ggaaaagacc aatccgccac
    26761 caaatttttc ttgccgttat ttcacatact caaccgaaca aattatttgg gcaagaaaat
    26821 ccgaaaaagt tcctcattac ttcaactatg aattaatgaa gcagctaaac ggaaacaagc
    26881 aaatgaaaga tgtttggaga cttccggcaa ttgctccctg ggaaaaatcc tgtggaaaac
    26941 acccgaccca aaaacctctt tcggttttaa caaggattat tttagcttcg acaaagccaa
    27001 acgcttggat tttagaccca tttacgggaa gctctacaac aggaattgca gcaaaccttg
    27061 caaacaggcg gtttttagga attgaccagg aagaagattt tttggcaatc agcaaaaaca
    27121 gaaaacttga aattgaaaac cctaaaacag cagcaacatt cagacaaaaa attggtggtt
    27181 tcaacgacaa gaaagggctt gaattatttt tagtagaaga acctgaagaa gaatataaaa
    27241 cagagttaaa cttgacgaca aagggcagca tacaacaata gtatggtata atggcgggcg
    27301 atgtgtttaa ttgatcgata atgtttctaa atccgctatt acgccaagtt gcaaaacgat
    27361 atcgagcttg aggtcgaaac ggttcgtaag taaaacccct tgctccgccc tctggcaggc
    27421 aagggttttc aaaaacaata attgacctta acctttaaaa accaaaaaac caatttgaaa
    27481 ttgtagatct tgttatttca cttaattata cagcccattt ttttttcacc ctacttattt
    27541 cctaaaaaag tgtaaaaaca ttttctgtat tgtatttatt ggtaatacag tgttttattt
    27601 tgcacaggtt cttaaatcat tttttattca taaattttat tacatattag ggaaaacaga
    27661 ttaacatctg tattaatatc aaaacaagta aatacgtggg aatggatatt cagagaacag
    27721 gtattgacga aattgacttt gaccagattt ttaaacaatg gtatggtgct atccggaatt
    27781 ttgtgtatta taaaattggt gatgtaacta aagctgagga tattgcgcag gatacatttt
    27841 tgaaattgtg ggaaaaacgc gataccataa aaacagaaac cgtaaagtca ttgctttaca
    27901 ccattgctaa taacttaacg attaatgtaa tagagcataa caacgtgact tttaagtttg
    27961 agaataaata taagccggac accaaacacc catcaccaga gtatgatatg gaaatgaaag
    28021 agtttgatca gcgcctgcaa caggtattag gccagcttga tgagaagcaa cggacggtat
    28081 ttttgatgaa ccgcattgat gaaatgactt acaacgaaat agcccaatcg cttggtttga
    28141 cggtgaaagc gattgaaaaa aggatggaga aagcactgtc atatttaaga aagaacatag
    28201 cagtaaaaat ataacaccta cggcaatgac accaacatac gcaaataaca atatagatga
    28261 acagatactg gatacggtat ctggctatca gccaccgttt gaaacaacaa cggatgaggc
    28321 tctggcaata ttgaagaaaa agattgccca gggcgcaaaa gctaaagatg tgaaggaatc
    28381 caaaactata ccattcaaaa gcattgtgct gtatgcttct ggtattgcag catctttgct
    28441 tgccattgtg gctacttggt atttcttttc atacaatgcc agggtgacag tattggccga
    28501 caaaggccaa catactgaat gtgtattgcc tgatggttct gtgataagcc taaatgccaa
    28561 aagcgctgtt acttatcgta aacaaacttt tcggcaagaa cgctttattg aaatggaagg
    28621 agaggccttt ttcaatataa agaaaggttc tgtatttacc ataaaaacta agcatgctga
    28681 cattaaaatc ctggggacat catttaatgt atatgcccgc gaaagtatgt ttaaagtagc
    28741 ttgtgttacg ggccgtgttt tggttgccaa tgttgaagat accgttatta ttgaacctgg
    28801 tgagagcgct tctttattta acaggtcatt aaagaagttc cgtgatgaaa aaatagcttc
    28861 ggaaacaaaa tggcgtaacg gagaatttat ttatgaaaat gcctcgctta aaataatctt
    28921 tgaagaaata gaacgtcaat ttaatgttaa ctttgccctt cctgatgatg ttgaaagcaa
    28981 atattttaca ggaggtttta gcaacaaaaa tttagtcgat gcgttggata tagtatgtat
    29041 cccgatgggc ttaacttatg aaattggcgc taatggttat atatcagttc acgaaaagta
    29101 ttaaaagtat tgttttatgg gtgttaacgt tattgtttat tggtacattg cctgttgatt
    29161 cccaacaata caaactttca ttgcgaaaaa caaacttggc cgatgccatg gaacaaactt
    29221 caaagctact caacatcagg gtggcttttg acgctcaaaa actttcgcaa atagccattg
    29281 ataaagagat ttcgggcaac acccccgatg aagtacttaa aaaacttctt gcgaattcgg
    29341 gttatagctt tgaattcagg catgacaact ttcttattta taaaagtgag gaaaaatccg
    29401 atgaaaatgt gtgccagatt ataggttcca ttacagatat tgaaacaggc gagcaactgc
    29461 cttatgctac tataaaaatc aaggatcaaa atatctatgt ttcgtcttct gaaaatggca
    29521 ctttctatct gaaacaaagc aatgctaaaa gtattcagct caacattagc tatataggct
    29581 attatccttt ggatacgctt gtttattgca atgcacccaa agtggtttgc aagctggcct
    29641 tgaaaaaaaa catacaggag cttgaaaagg ttgaggtgcg tgagccaaag attgaaatgg
    29701 tagattaccg caatgatgtg gactttgcta caaccatcaa tgccagcaaa ttgctggatt
    29761 tgccaatgtt tgccgaaact gacattttca gggctttgca gttgttgccc ggggtgagct
    29821 tctccgaaag ttcctcagag cttaacatcc gtggtgggag cagcgaccag aaccttgttt
    29881 tgctcgacgg acaaacgttg tacaaccaaa gtcattactt tggcgtattt tcagctttga
    29941 accctaatgt tgttaaagat gttcaggtgt ttaaaggagg ttatgattcg cgttatggcg
    30001 agcgggtttc gggcattgtg gatattaccg ggaaaaatgg gaacaagcta aaacccacag
    30061 tgtatggaga tgtcaacctg ataaatggta atattgctgc tgaagtgcct gtaagtaaga
    30121 agttgacggt tattatggcc ggaaggcgga cgtatggtga tatttatagt accgagtttg
    30181 ccaataacct atttgaaaag gacagcaaca ggttttctaa tccttcgcaa cgcagcctca
    30241 gctactctaa gcctaagttt tctttttacg attataacac taaggtatca taccagctga
    30301 atgatggcga aaacgtttct ttttcggtat atggggccaa agatagctat gataacaaat
    30361 acaatacggg agacaaaaac tttttggcct ctattcacga aaacaataag tggggcaact
    30421 acgggctgaa tgcttcgtgg ctcaaacagt ggaatggcgc actgtttaca aatgtccaga
    30481 tagggagctc gggctattca gatacataca atagtagctc aagtttgtca ggcacaggcg
    30541 gccctcatta tccttccggc ccgccttttc aaaataaaaa ttatgattcg tatagttaca
    30601 acgagctaaa tgattattcg gcttctgtaa aaggcatctg gaaccagaac ggtaaacatc
    30661 agttcagttt tggggcaaca gtaaggcaaa ataaaatata ctatcataaa gatgccgaca
    30721 gggtctatat ttacgaaaac atgaaccagg cagctgtgat ttcttcatta tttctccaag
    30781 acaggctgat aattacagac aggctcacta ttaagccggg tttgcggatt agcttctatg
    30841 acggcaataa tggatggtat tctgaaccgc gctttgctgc aaattacagg ctgactgatg
    30901 cctggtcggt gcggtttgca accgggcgtt tttaccagta cataaaccag gtgcaggtac
    30961 ttcaggagac cggatacaat aaaaactttt gggtgttgac agacgatagc atccacccgg
    31021 tggtaaaatc caatcattat atattcggca catccattga cttggggagt ttcttttttg
    31081 acgttgagcc gtattacaag acttatagcg ggttgcagga atatatcttt gtatccaact
    31141 acacccgcga ctcaactttc cggaacttgt ttgatactgc caatatgcag atgtttgccc
    31201 caaaagaaag gccggggctg tttattcatg ggcagggtaa gtcttatggg atagactttc
    31261 acgtgcgtta taaatacaac aactttacca catggctttc ttactcgctc agcaaaagta
    31321 cccgtcagtt ttctgagata aataatggca acgaagtgcc tgcatctaca gaccaacgtc
    31381 accaaataac ctggacaaac atgtacgcat ttaaaaattg gaattttggt ctaactaact
    31441 attattcaac cggccgccct tacgttgacc tgagcataga tgcccggcgt atgcctacta
    31501 tccgctctta taaacggttg ccagattatt accggacaga tatttcggct aattataatt
    31561 tcaaaatcgg cgaagcacgc attaaagcag gggctaccat tatcaacctt ttcaacgtgg
    31621 acaactacta tgatgtcaat tctcgaaagt ttgacaccga aaatgccagt ttttccgaaa
    31681 ccaacgttgt cagggcgcaa gacaggtctt taaacctttt tatccatttt gtattttaaa
    31741 aaatagggga tattctttat agcttgtaat actgatataa cttaatattt aaggctatga
    31801 agaaaaaact tttaatcctt gtttgtgcaa tggctatggt agcttttgca ggttgtcaga
    31861 agtctgatga aaaaagcgaa aatatctctg ctttaaattc actttcagaa aatgttgcaa
    31921 ttgctgcaac cagtgtttct acaagccaga tggctctttc atgttctgaa gatattcagg
    31981 ctacggtact ttcagatgtt gatagttcat accgaaaagt gaaatcttat tttcacaggt
    32041 atggaaaagg ggctttcaag ctttgtttgt ctaatattcc tatttgtgct actgtaactg
    32101 ttagtagcga tacatttccc aaaactatca tcattgatta tggtacatcg ggttgtacaa
    32161 gtcaacatgg caatgtaaag acaggtaaga ttattattga ggttactgat actattatta
    32221 atgccggagc tgtaaaatcg attaagtatg ttgattttta tgtaaatggc aataaaattg
    32281 aaggttctgg gattatccaa aatctgggca aaaatagcaa tggtaactgg gtattggcta
    32341 caaatacaga gtttactttt accgattcga gcaacaatgt agccaaagag atttataatg
    32401 ataccattga atgggtaggt ggttttgaaa ctgctgataa atctgacgat gtttattaca
    32461 aatcgggcgc aggttctatt accctgaacg ataccattgc ttatagccgt actataacta
    32521 ctcctttatt gatgaataat acatgtggtt atataattag cggggtagaa acactttaca
    32581 aaaatgggag tacagtagtt attgattatg gcgatggaac atgtgatgta gttgccactg
    32641 taacaataaa tgggactact gaagaaataa gtatagaaaa acatcacttt aagaaaggag
    32701 gccattttga aaaacaggtt ggccatagca aagggaacaa aaaagggctg tttggctttt
    32761 aaaagaggtt tggtttgatt ggaagggcac tcgcgataat gagtgccctt ttattttgag
    32821 ataagcatct ttattttatc aaaaagagca gtattttggt aacatccggt aaacatttct
    32881 gccccggggc cataagcaaa aaccataaca ggaacggcag tatggttgag cgaagaaaag
    32941 tttgagtcaa cagtactttt tttgaagttg ccgtttatta aagttaagcc tccgctttcg
    33001 tggtcggctg taacaataac aagggttttt ccatcttttt tggcaaaatc aagggctacc
    33061 ttaacagcct tgtcaaaatc aactgtttcg ttgataacat aattgatact gttgaggtgg
    33121 ttgccccaat ctatttgaga gccttctacc attagcaaaa acctgttttt tttcaacagc
    33181 tctattgatt tttgagtagc taacggtaaa aaatcatttc ttccttcaga aatttttggt
    33241 aaatctccgt ctgttgccag acataacact ttttgctgcg aaatatctac agagtcaata
    33301 ttagtgctgt aaaagtaacc gtctttcatt aacaactcaa taaggttgcg tttatctttt
    33361 cttttgacaa aatatttgcg gcccccacca aaagcaacat ctacagtctt gttcaaaaaa
    33421 tgagaggcta ttgtttcata ttcactgcgt ttcccaacat gggcataaaa tgcagcaggg
    33481 gtagcatggg taatagcaca tgttactgca ataccagtag agtaatgttt ctctttggca
    33541 tattcaaaaa tggtttgcat aggctttccg gcagtatcaa cagataagct gccattattg
    33601 gtttttttgc cacacgcaaa agctgtgcct ccggctgcag agtcagtagt atattcgtct
    33661 gccgagtatg taagcataat gccggttaca ggcattccaa atatgttcag atattggcca
    33721 tttgcggtgt atgctgcata catctgggca attcccatgc catcgccaat catcaatatt
    33781 acattttcag gaagttgttc tttgaaggtt tcttgctgtg catttaaact attgacagat
    33841 agtgcaatac agcaaatagt taataatctt ttcaaactca tttttatgtt tattctttcc
    33901 atttcaaaaa tatatctttg ttttgtaaaa aagagtaaaa cacaatatta ataatttaaa
    33961 tatagttcat tatgggactc aacattgtaa aagactattt tcaaaaatca ggtaaggagc
    34021 agaatatcat gctttttacc ctgaaaaata ccgaaggtac agaagtggca ataacaaact
    34081 atggcgcaac cattaccagt attaaaacta aagacaaaaa tggcaagcca ggtcagatag
    34141 tttgtggctt tgataatatt gaagattttc agggcaacca tcctttcttt ggagtagttt
    34201 gtggccgtta tgcaaaccgc attgcaaaag gcaaatttac actcgaaggg aaagaataca
    34261 ccttggctat taacaatgct cctaatacat tgcatggtgg caataagggt tttgataagg
    34321 tagtttggag ttttgaagtg gttgaaagtg cccaggctgt tggggttaag cttacttacg
    34381 ttagcaaaga tatggaagaa ggatatccag ggacattgac agcaactgta acatatcttc
    34441 tcaatgaaaa caatgagttg caaatacatt atactgcaaa aacagataaa acaacagttg
    34501 taaacttgac aaaccatgcg tattataatt taggagggtt tgctggtgaa ataactgacc
    34561 acttattgca aataaacgct aatagctata ccgaaacgga cagtactagt atcccaacag
    34621 gtaaaattat tgaacttgcc ggaacagctt atgatttcag ggaactgaca cttataggtg
    34681 acagaatcaa aggtacaacc aatggttatg acgacaactt tatcctgaac aaggcaggac
    34741 agccagcttg ctcatttgct gctaaggttg tccatccggg aactggccgt accatggaag
    34801 tatatactac tgagccggga gtgcagttct attctgccaa ttacctcgat ggcactatca
    34861 aaggccatga tggcgtagtt tacaaaaaac gttttgctct ttgcttagag acacagcatt
    34921 ttccagattc accaaatcat gcacacttcc caactacggt tttaaaacca ggtgaaactt
    34981 atacacaaac tactattcat aagtttgggg taacagaaaa gtaaaacgcc tgcgcttctg
    35041 gacaaaattg aacgctaaga cgcaacaacg caaaacaaat aatactttgc gtcttcgtgt
    35101 cttagcgttt agtttaaaat acagcttatt taatcccaca catatttcca gcttccatca
    35161 gcttgccttt tccaaacagt gtgaaatata cctttgcttt cggttgtttt tccaaagctg
    35221 tctgctgatt tccaggtgta ttttccataa gtgtagccta atgtcccgtc atcagaaact
    35281 tctataaagt ctggagccca tgatacttca gcatttttag ctgtattttc ttcgtaatat
    35341 tgttttatag cggctttacc cttaatcaaa ttgttttcac cacgaatgat gacagcgcta
    35401 tcatctgcaa actctccaaa agcctctgca actccaatac tttttgtcgc agcctcaaat
    35461 gctttctcgg ttttaaaaat ttcttccttt acagcttctt tatttatctt ggttttacaa
    35521 gatgagataa atgtaagcat tgtaaataca aataataggc taattatact tctcatagtt
    35581 tttaaattta ttgtaacttt tacataacag ttaaaactgt cggattctct tactaacaat
    35641 gattaaagat aagaataatt aaaaaatcaa aaaaaagaaa gattttaatg gaagtattca
    35701 gttgtttctg ttattttgca acgctaaaat ttttaaaagc aagaaatgat gcaaaatcag
    35761 gaaatagcta ttattggtgg tggaagctgg gcaactgcat tagccagcat cgtatcacac
    35821 aatgtaaaga agttccattg gttcatctac gaaaaagaga ttatcgacca cattaaaacg
    35881 cataagcata atcctcaata tatcagcgct atagagtttg acccttcaaa gattgttttt
    35941 cacaatcatg tgaaagaagc cattgatgcg gccaatgtca ttatttttgt tgtgccttcg
    36001 gcatttatca agccattgat gggaaaagaa gatattgact tttcgggcaa attctttgta
    36061 aatgctgtaa aaggccttat tccggacgat aatattactg taacccagta ttttcaagaa
    36121 caattcagca tcccttttag tagggtaggt gtggtttcag gaccttgcca tgcagaggaa
    36181 gttgctttgg aacgtttatc atacttgaca gttggtgctg agtttgaaga agatgctaaa
    36241 caagtagccg atttgataag ggctcctttc gtcaatacta ttatttcaaa cgatatatat
    36301 ggcattgaat atggctctgt actgaaaaat atttatgcac ttggtgctgg tatcaaccat
    36361 ggccttggat atggcgacaa ttttcaggca gtgtatgtgt caaacgctat acgtgagatg
    36421 gaacgtttcc ttggaaaggt atttgaacgc gaacgccatc ttttccattc agtttacttg
    36481 ggcgacttgc tggtaaccgc atattcacag tttagccgta accgcacttt cggaaacatg
    36541 attggcaaag gctattcggt tcaggctgct atgattgacc tcaatatgga ggctgaaggg
    36601 tattacgcaa ctaaaagtat ttttgaggta aataagaagt ttaaggtcaa aatgcctatt
    36661 gctgaagcgg tgtataaaat attatatgaa aaatgcaatg ctgctgctga ggtcaaagcg
    36721 cttgcccaaa agttgaaata gaaacatcaa cctataaaat atcttgatat gagtacttta
    36781 aatctaagca ttgataaaat ttttgacttt gtcagtaaag acaaagtgta tgcatttgca
    36841 gacgaatgtg tgaaacaaaa taaggctttg cacgagaaaa ctggcaaagg caatgatttt
    36901 ttaggatggg tgaaccttcc gtcttctata actcctgaaa ttattaaaga tattgaaaaa
    36961 acagctgcat cttttcgtaa aaattgcgaa gttgttgtag tagttggtat tggaggttca
    37021 tatcttggcg ctaaatctgt aattgaagcg ctctcagaca gtttctatca gattaacagg
    37081 cccaaagata cacctcttgt agtttttgca ggccaaaaca ttggtgagga ttatactgca
    37141 gaactgataa aattattgga taaaaagaaa tacggcatca tttgtatttc aaagtcgggt
    37201 actacaaccg aaccagctat tgctttccgt atccttaaag gacatttgga acaaaaagta
    37261 ggtaagcccc acgcaaaaga gcttattgtg gctattaccg atgcaaaaag aggcgctttg
    37321 cgtacccttg ccactcagga aggttacaaa acctatgtaa ttcctgacga tgttggtggc
    37381 cgtttctctg tactcacccc tgttggttta ttacctattg ctgtggccgg ttttgatatt
    37441 gccaaacttg ttgcaggtgc ccaaaaaatg gaagaactaa ctgctcctga tgttgctttt
    37501 gaagataatc ctgctgcaat ttatgctgct gcacgcaatg ctttgtacaa agcaggaaag
    37561 aaaatagaaa tccttgcaaa ttataatcac aagcttactt ttgttgccga atggtggaaa
    37621 caactttatg gcgaaagtga aggaaaagaa aataaaggca ttttccctgc cagcgtaact
    37681 tttacctctg atcttcactc aatgggccaa tatattcaag aaggcgaacg tcatatcttc
    37741 gaaactgtta tcactgttaa gaatacaaac cacaatgtgg ctgtcccttt tgaaaaggat
    37801 aaccttgatg ggctcaactt ccttgcatac aaacgcattg atgaagtaaa caagatggcc
    37861 gaacttggaa ctcttgtagc ccacatggat ggaggtgttc ccaatatcat cattgaaata
    37921 tccaggcttg atgagtataa tctcggactt ttgttctatt tcttcgaaaa agcttgtggt
    37981 atcagtggtt acttgttaaa agttaacccg tttgaccaac cgggcgttga agcatacaag
    38041 aagaatatgt ttgcattgct cgataaacct ggttttgaaa cagattctga aatattgaaa
    38101 aaacgtttag gcatcaacaa ataatatcaa taattgcaga gcttgtttat gcaggctctg
    38161 ctttttttct tggttcttga ctcttggttc tctaatatgc tcgaagtaaa aaacatacat
    38221 aaatcctttg gaaagctcga agtgttgaaa ggtatagatc tttcgataaa aaaaggcgag
    38281 attgtgtcta ttaccgggcc tagcggagca ggaaaaacca cgttattgca gattatgggc
    38341 acgcttttaa aacccacctc cggtaaggta tttattggtg gtaaagacgt gtttgctatg
    38401 aaagatgacc agcttgccgg cttcagaaat agcgagatag gttttgtctt tcagttccat
    38461 cacctgcttc cagaatttag tgcggtggaa aatgttatta ttcctgcatt aatcgctcgc
    38521 aagaaaaaga aagatgccat agaaaaagct aaatcattac tcgaagaatt aaacctcggt
    38581 catcggttga accataagcc gggcgaactt tcaggaggtg aacagcaaag ggtttctgtt
    38641 gcacgtgccc ttatgaacga cccgctgctt gtgttggcag atgaaccatc cggaaacttg
    38701 gattctgtgc ataaaacaga attacatcag ttgtttttcg acttgcgcga ccgctacaat
    38761 caaactttcg tgattgttac ccacgacaac gagttggctg ccaaagccga ccgccagata
    38821 aaactagccg acggccttgt tattgcccaa taatctgata aaagatttac aagagataaa
    38881 gcagtcgctt gattggcttg tcattcattt caatactcct gcatttattg aaaaagaccc
    38941 tatatctgtt cctcacaggt tcagtaaaaa ggaagatatc gagattgctg catttctggc
    39001 agccactatt gcctgggggc agcgtacaac gatattgaac aatgccaaca aactcatggg
    39061 gttgatggac aatgctccgt atgattttgt taccggccat actcaaaagg atttgcagcg
    39121 tcttaatggt tttgtacacc gcacgtttaa tgaggtggac tgtatatttt ttataaaatc
    39181 cttacaaaat atctattcca ggcataatgg gcttgaaggg gtttttaata ccgggtttca
    39241 tcatgataat actgtttttt cggctttagt gtattttcga aaactatttt ttgaaaccga
    39301 acatcaatac cgaagtgaaa agcatatttc tgacgtaaca aaaggcgctg cagcaaagcg
    39361 catcaacatg tttttgcgtt ggatggtacg tcaggacaag tgtggcgttg attttggtat
    39421 ctggcgaaat atcccggcct ctgcattaat gctgccttta gatgtgcata cagctaatag
    39481 tgccaggcag ttggggcttt tggtgcgtac ccaaaatgac tggaaagccg ttgaggaaat
    39541 tatgtccgtt ttgcgtaaat ttgacccgca tgaccctgta aaatacgatt ttgcattatt
    39601 tggggccagt attgaaaaga tttttttaaa gtaatacaat tataaacgtt ggcaaattct
    39661 tattttcagt ttaaaaaatt taccatcaac caggaaaaat gcggtatgcc tgttaatacc
    39721 gatagtgtat tgctcggggc atgggctaat gttgataatt gtaataaaat attggacatt
    39781 ggcactggaa ctggcatttt atctttaatg atggcgcaac gttgcaatgc aaccattgat
    39841 gcagttgaga ttgatgaagc atcttatttg caagccctta ccaattttga acagagcgat
    39901 tggggtgacc gtataaatgc ttatttctgt tcaattcagg agtttgctgc ttctacttcc
    39961 agtaaatacg agctgttgat atgcaacccg ccgtattttg ccaatgcagt aaagagcacc
    40021 aatcatcaaa aaaagacggc aaggcatacc gatacgctta gctttgaaaa ccttatcagc
    40081 gctacaatgc atttgcttga agataagggg cggctatcat tgatattgcc ttattccgaa
    40141 ggctgcatct ttattgcttt agctgcaaaa gaaggattgt tctgtgtacg taaaaccaat
    40201 gtaaaatcag tgactcatgc acctataaca cgtttattgc tcgaatttgc tacacttcct
    40261 caacaagtag cagaagatta tcttgttatt gctgatgaac agaacaatta ttcagataag
    40321 ttcaggcatt tgacagttga tttttataag tttttttaaa atacggttat ttctgttaag
    40381 aggctgttaa agaatatcat tcagctgctg taatagtgta aatttgcccg taaattaata
    40441 aattgcaaag acgttagaag ttttatttat ttttcatgaa aaaccgcgca attactatta
    40501 tcgtcgtttt gtcttcaata gcaatgttgg ggcttattgc agcgcaggct tattgggtaa
    40561 ataaagctta tgacctgtcg caaaagcact ttgaacatag ggtttattca ggattatatg
    40621 gcgctatgct cgaaatatca tggaacaaga aacctatgcc aaaagattcg gccaatatga
    40681 ccgaagctca gattttactt gccagagtac gtccaggatc tgttgatact tctctatatc
    40741 gccattttgg ttataaccag gtggataata aatacgaatt tgctatagtg ctaaacagta
    40801 acgattctat tgtttaccaa tcgccgggtt atgtaagaga tgtaaaaaaa gaaaaggttt
    40861 ttaaaaagtg tttgtggtac atgccttcta tgaaaaactt tagtatcagg gtcatatttc
    40921 ctcagataca aaaaaccttg gtgggcgata tttggttttg gctttttttg tctggttttt
    40981 ttctgatggt ggtaattctt tgttttgccg caatacttat attggcttat aagaacaaaa
    41041 agctttcaga aatgaagaca gactttatca acaatattac tcatgagttt aaaacaccaa
    41101 tcagtacaat atctctggct tctgagattt tggtaaacgt aaaccaggct tctcacctgg
    41161 ataaagtaaa aaggtatgcc agtattattc aggaagaaaa cagaagaatg caaatgcagg
    41221 tggaacaagt tcttcgtatg tccaaactgg ataaaaagga gtatgagctg aacaaagaaa
    41281 atatagacct taatgaactc gttacaaatg ctgttcaaaa cctgtgtctt gatttggaca
    41341 accctgcaat aaaaatacac ttttcattgt ctgacaaaaa aattgaaatg ctggccgacc
    41401 ctgtacattt gaccaatatt attaaaaacc ttgttgataa tgcctgtaaa tattcaaaag
    41461 gaaacccgca gattacagtt tgcacaagct tgaacaatac gtatgctacc atttctgtag
    41521 ccgacaaagg aataggcatt gaacatgaca agcaaaagta tattttcgaa aaattttatc
    41581 gcataccaac tggcgatgtt catgacgtta aaggttctgg tattgggctt tattacgtta
    41641 aggttatgac tgaagcacat gggggaaaag taattgttaa aagtgaaccg ggcaaaggtt
    41701 cccaatttga tatagtttta cctcttaaat aattgacaac ccatataaaa tgaaaaacaa
    41761 aggaaacata ttacttgtag aagatgataa aaatcttggg tttatcatca aagatttttt
    41821 acacgaatct ggttaccatg tagatctggc cgaaaatggg cagagtgctt tggccatctt
    41881 cgggactaaa agctttgatt tggttctttt ggatgttatg ttacctataa ttgatggatt
    41941 ctctgttgca gaaacaataa aagctaagaa tgctattgtc cctattattt tcttaactgc
    42001 acgctcattg gatgatgata aaatacgcgg tttgaaaatt ggcgctgacg attatatcac
    42061 taaacctttt agtacagaag aactattact ccgtgttgaa gctgtattgc gccgcacaaa
    42121 aggccatgtt gccaatgttg ttgtttcgca agaaatattt caaatcggtt cttatacttt
    42181 cgactcatct aaccaaacta ttacccttgg agatgatgta aaaatgctta cccgcaaaga
    42241 agcagatttg ctaaggcttc tttgtataaa caaaaaccag cttgtacgcc gcgattttgc
    42301 ccttaaactc atttggggcg aagatgatta tttcatgggc aggagcatgg atgtttatat
    42361 cactaagcta cgtaaaatat tgaaagacga cccgtccatc caaattcaga acgttcatag
    42421 gactggtttt aagcttgaag taaaagacta atgcttagaa agggtaacca tgattggcct
    42481 tatctttatt ttcttcaata atagctgaat tttcttttat ctcattggtg gtatcctgca
    42541 cttccttttt taaatgggat gtttcgcgtg ctatctcttc ttttatttca tcgctggctt
    42601 tctttatttc gttaataccc ttgcctaagc tgcgtgcaaa gcccggaatc ttgtctgcac
    42661 caaataatac aagtatggca agtaaaatca aaagaatctc agaaccgccc attttcttat
    42721 ttttgtacaa atttacttat tttcagtcaa tctaaatatt aaagaaaaat attggaatct
    42781 ttgtttatta ttcattattc atgatggtac ggctcattat tgataatggt aaaagccctg
    42841 tatagctgtt ccaaaaaaat agtccttaca atctgatgcg aaaatgtcat ctttgaaagc
    42901 gatatcttat attgtgccct gtttataacg gattcgtcaa agccataagc accaccaata
    42961 ataaaaacaa gccttttaca acctgagttc atttgtccct gaataaaact ggcaaattca
    43021 ggtgaagtgt attggttgcc ccgctcatcg agcaagacga ctttgtctgt tggttcaatt
    43081 tgctgcaaca gcaagctggc ttcctggcgt tttattacct gggtatctgc tacattacct
    43141 tttttctggg taacttcctt gacctgaaag ttcaggtatc gcgatattcg ttctgtgtat
    43201 aattgaatac cttgcgaaac aaaggtgaaa tctgtctttc cggtaaccag cagtaaaact
    43261 ttcatgaaat gttgttttgc attttgaaat atcattattt tgcggcgaaa tgcaaaggta
    43321 ctgaagattt tcaattgaaa tcggggttaa tttctactaa tatttaacat agttttgtgc
    43381 ttagtttttt ttaatatttt tcactacttt tgccttcatt tttttactat ctttaaaagc
    43441 aaataaaaat aagtgccatg tttgaaaaag aacttgaaat atttaagaaa ataaaagccg
    43501 atttactttc tcaatattcg ggtggatttg tggttattaa aggtgacgaa gtattaggcg
    43561 tatggcaatc aagattagat gctcttaaat cagggattga taaatatggt gacgttccat
    43621 ttttagtcaa aaatattaat gagagcgaca tagttatcaa tttttcaaga aatttaatat
    43681 ttgcataagt atgcctgtgt tccctaatag ggtgcctaat ttacaacaaa ttggcccaat
    43741 agttgaagtt gtaatatttc cttcacaacc tatctatata aaaatgcgaa atgaaggcca
    43801 aaatccacct tcaaaaaaag ttattgctct tattgataca ggagcaagtg gttcttgtat
    43861 caatgaccca atagcaaaag aattaggttt aattgctcgt gatgtagttc ctgtccaaac
    43921 accttctggc acatctcaac aaccaatcta cgatttagga ttttctcttc caagtttaac
    43981 tgcaaatatc atccctatac aagcattagg ctctgattta gaaaaacaac catatgatgc
    44041 attaattggt agagatattt tatctttttg cactcttatt tataatggtt gggataattc
    44101 atatcagttg cacatctaaa caaatgcaca gagctgtttt gacattacat acattatgga
    44161 aatcaggaaa gaagatattt taccattttt aaaagttgtt attgccgaag atttaggcga
    44221 tggagaccat acatctcttg ctactattga ccctgaacaa cgagaaaagg cccagttgat
    44281 tgtaaaacaa gaaggtatca ttgctggcat tgatgtagcc cgtatggtgt ttgaattatt
    44341 agacagtaca gtaaccttta ccccattgat gcaagatggc aggcaggtta agtttggcga
    44401 aatagccttt attgttgagg gacggacaca aactatgcta caggccgaac gtattgtgct
    44461 caatattatg cagcgcatga gtggtattgc aacccagaca aatacttatg tcaagaagtt
    44521 ggaaggaacc aaaaccaagg tgcttgatac ccgtaaaaca actccttgca tgcgactgct
    44581 tgataaatat gctgtcaaga ttggaggggg cgagaatcac cgaattggct tgtatgatat
    44641 gatattaata aaagataacc atattgattt tgctggagga attgaaaaag cccttgaaaa
    44701 aacttatcag tacctcaaag aaaaaaataa aaacctcaaa gtcgaaattg aagcccgttc
    44761 gcttagcgat gttcagcgaa tcctcaattt tggcagggtt gaccgcatta tgcttgacaa
    44821 ttttaccccg gctctaacta aacaagccgt agaactcatt gccgggaagg ctgagattga
    44881 atcatctggt aatattacca ttgaaaatat cagggattat gccgaatgtg gcgttgatta
    44941 tatttctgtt ggcgcattaa cgcaccaaat caagagcctt gacctcagtt tgaaagcaat
    45001 taagtaaatc ttcaattaac aattattgct ctaaatcttc accctgtcaa aaccttcgcc
    45061 atatacgccc atgacgctgc tcatagaaat aaaggcttgc gggtcttctt cattggtaat
    45121 acgcaatacc gctgctgact cgtatttgcg tacaacaatg agcaatatgt ccatgttttg
    45181 tttagagtac cagccttgac cttttattaa agttatgcca cggccaactt catcggatac
    45241 ccgttgggct atttgctcgt gtttcttgct gaaaataaga atttggtatg attgattggt
    45301 gccttcaaga atcaggtcta taacatacga ggtgatagcc attgttacgt agccatacac
    45361 tattttttca agactataaa acagaaagta cgatgaacca atgataataa catcctgata
    45421 aaggattaga cgtcctaatc ggatgttttt atatttatgg ataattaaag ccgttatatc
    45481 tgtgccacct gtactacccc cttgcgaaaa aacaagtcca acaccagcac ctgataatat
    45541 tcctcctata acagttgcca taaaagcatc tttaacaatc ggagctgtaa aaaatggctg
    45601 cagtatagta aagaagaccg acaatccaat aaccccatat accgtttgta ttccaaactt
    45661 tagacctaaa atacggatgg atattagtat aagaatgacg tttaaaatca gatacgtaag
    45721 ccctatgggc agtttgatag cgtaaaacag cagggcacat aagccactca atccgccacc
    45781 tgttatctga gcaggtatca aaaatgccat ccagccaatg gccgtgagta ataggccaaa
    45841 ggtgatgatg gcatgggatt ttatcttttg ttttaaatct ttcggcatac cagtttcaaa
    45901 tgaactggta ccaaagataa tttaatatgc tggttttctg ctataaagct tcgactattt
    45961 aaaaaaatca atggttgtta tcaaatcttt tgaacgatca gccaataatg ttgagctttc
    46021 agataacctt tccgaaacta ccaccgtctc ctggcttgag ttgttaagct cttgcataga
    46081 gttgtttatt tgctgtatcc cgataagttg ttcgctacaa gcagaggaaa tttcctgcat
    46141 aaaggaggag ttgttgtcaa tgttaggaat caggtcgatg aggagctggt ttgtctttga
    46201 agttgactct accatttgtt gtaccaatgt ttgaatctcg tcggcagcaa ttttgcttcg
    46261 ttctgcaagc ttccgtacct ctgcagcaac aactgcaaaa cctttaccat gttcaccagc
    46321 cctggctgct tctactgctg catttagcgc aagaatattt gtttggaatg ctatgtcatt
    46381 gataatggta attctttgtt ttattatctc catattcgaa aagctttcag aagatacagt
    46441 gcttacttct ttaagttttt caacagaggt tttggctact ttatctgtgt tagctgcatt
    46501 ttggtgtgtt tgtgccaggt gggcactcat ttcttcaaca gatgaagaaa ccatttctgc
    46561 agtggcagct tgttcgctgg caaggctggc aaggttttct gcatctgaag taagcgcctg
    46621 gcttgcttca gaaaggtttt ttgaagtttc gccaatttgg gttaccaggc ggcgtaaagt
    46681 gcttttgtgc ctgttcagcg cttttgatat atctcctatt tcatcttctt tgtcatattt
    46741 caaatcttcg ttttcaatag tcccttctgc taaagcttct aattgagaac gtatcatttc
    46801 aaaaggcttg cgcataatat tgcgcatcat gatataagaa attaggatgg ttaaaagtac
    46861 taatggcgca gcccaaaaag catgggttaa cgacgaagtg ccaatgtaat aagcaataca
    46921 aataataatt acatttgatg cggtaataga atgtgtggca tttagtacaa aggacctttt
    46981 aaagacaatt cgttttatta cagcaaataa aatcaaactt gtcgttacaa taatgataaa
    47041 aagcgttgtt ttgtccatgt tcgtataggt attggtttat agtaggttcg ctaaaattat
    47101 actaaaaaaa aatatctaaa caacttttgg ttttatttta gttcagtaaa atgcttaata
    47161 gctttatttt attggcaaat aaaaaggtca taaaagaaat ctcctttatg acctttttta
    47221 tagtaaaaaa ctaattgcta tagctcgata acaaatcctc ccattggttt aacctgtagt
    47281 tttacttcat tgttgttggt gttttcagtt ctgaaatctc tgtttgtttt gccatcagta
    47341 ataattacag catctttttt ttgtttaaat cctgaaatat cgaatttcag gttcaaatct
    47401 tctgaagtag agttaatgcc ggcaatatac cacttatcat ttccttttct ggctattact
    47461 acatatttcc cagggtatcc atcaataaat tttatatctg accatgaaga aggaagtgtt
    47521 ttgataaaat ccttaacaaa ttcaggttgt ttggccattc cttcgggaac ttcagcataa
    47581 tgttgtatcc cggattggaa taatacagaa agtgccattt caaaagcgtt tgaggtattg
    47641 cgttttaggt taggtatttc agaaaaacac atcggggtaa aatccattgg cccaacaacg
    47701 ttacgcgtaa atggaagcat agcgcaatgt gcagcttgca tgtctgcccc ttcttgagta
    47761 aaagtacaga attcaaaccc tttgaccgct tccattgcca caaggttagg gtatgtacgt
    47821 gaccaacctc gggggactgt gcatccgtgc gtgtttacca acaacttgta ttttgcagca
    47881 tcctcaatca ggtcatgata gtatgctatc atcgactgtc cgtctcctcc ccagaaatca
    47941 actttaaccc ctgcaatacc cattttgctt attttttcaa actctttatt tcttattgct
    48001 ttgtcaaaca taaggttacg aggggtaagc ggggttgtat tccacgaacc ggctgagttg
    48061 taccaaagta taatttttac attcctgctg ttggcataag ccaccaaatc agcgattttt
    48121 tcataaccaa tttttttatc ccaaaaagca tctataaggc agtattgcca atgcatttgt
    48181 gatgcatagt cgataaaacg cttttgagtg ctgtaaatag tcgaatcgtc tttgtataaa
    48241 acccatgacc atgaagcttt cccaggttta ataaaagtag tgtcttttaa aacgcatggt
    48301 ttgctgacat cggtgataag gcttgattct actattgtac caagtgtttg ccctactata
    48361 attaccctcc atggactgac aaacgggaag gtaatttgag gtttcactgc ggccaagctg
    48421 tctgtacgtt ccataggttg tggaaactga atgctaaatt cggcatccgg cgaggctgtt
    48481 gatagccttg aaccgcaata atttctgtcc acatcagcct cggaaataag tacccagttg
    48541 ccagcagatt taaataaagc cggaaatgcc caaccagctt tatatggagc tttggttgtt
    48601 accggaactt ctgtgtaata atattgttca taagatggtt ggtcaaaatt ccagcctgtg
    48661 tgggcatctg tttgaggatg tagccaggcc ttagccgata atgggaagtt gaagcctgag
    48721 ttttctacaa taactgagtc tgattgattt ttatatttat ttatttcgta acgaaaagct
    48781 aatccatcat ttgaaacttg aaatactact gaaagcattt ctccatcctg gttttttacg
    48841 tcaatacgtt gctttattgc attgtatgag cacttttcgc gtttttcatt ttttaactga
    48901 taaatgtctt ttactttttc aacttcagaa gtgccaacaa ttgtaagccc tgtggtaaaa
    48961 tcagcttcag ataattttaa accaagtttt gaagattcta gcaccagcga atctttacat
    49021 tgtaagttgt aagtaaggga gcccaaagaa tcaatgctga tggtcacagc aatatttcca
    49081 tcaggagact ttatggtata acggtcagac accttctgac tacaggctaa gctaagcccc
    49141 aataaacata agacgataat ttttttcata ttttaagttg tttgtttgtg ataagttcgt
    49201 taatgcaaat ataattgtaa aaaatacact taataaattc tttttactaa agttataaaa
    49261 atttctgctc caattatttt caattcataa agaactcata aaaatgaaag cataattctt
    49321 gctatattcg cagaaagaaa aataacagaa tatgtacagg actaatactt gtggagagct
    49381 gaatttaagc catgttggta aaaatgttac gttgtcgggc tgggtacaac gggcccgtga
    49441 ccttggcgga atgacatttg ttgatttgcg tgacagatac ggaataactc agttggcttt
    49501 caatatggat aaaaacgctg agctttgtct tgaagcgcga aagcttggac gtgagtatgt
    49561 aattcaggtg tcaggtttag tggctgaaag agaaagtaaa aatcttaaaa tgcctactgg
    49621 tgaaatagaa atcattgttg attcgcttaa agtgcttaat actgctgatg ttccgccttt
    49681 taccattgaa gatgtttcgg atggtggcga tgaactccgc atgaaataca gatatttaga
    49741 tcttcgtcgt aacccgctca agcagaacct tatgttaagg cacaggctgg cacaggaagt
    49801 gcgccgttac ctcgattcta aaaattttat ggaaatagaa accccggtgc tgatgaaatc
    49861 tacacccgag ggtgctcgtg actttgttgt tccatcccgc atgaaccagg gccagtttta
    49921 tgcattgcca caatctccgc agactttgaa acaaatattg atggtagccg gctatgaccg
    49981 ctattttcaa attgtaaagt gtttcagaga cgaagattta cgtgccgacc gtcagcctga
    50041 gtttacgcaa atagactgcg aaatgtcatt tgttgaccgt gatgatatca ttgaaactat
    50101 tgagggttta atgaaatatc tttttaaaac ggttaaaaat attgaattta aagatgcatt
    50161 tcctcttatg ccttacagcg aagcaatgaa tttctatggt tgcgataaac ctgatttgcg
    50221 ttttgatatg cgctttgttg atattacttc tattgccaaa ggaaaaggct ttggcgtatt
    50281 tgataatgct gagtatattg gggctatttg tgcaaaaggt tgctcggaat actctcgtaa
    50341 agagctcgac gctttgacgg actttgtgaa gaagccacaa attggaggtt ctggactggt
    50401 ttatgtaaag tatggtgctg acgggtctat caaatctagt gttgataaat tttatacccc
    50461 tgaagatatt actgtatggc taaatgcagc taaagctgag aaaggcgact tgctgcttat
    50521 tgtctgtggc cccaaagaaa aaacactgaa agcacttggt gaattgcgct tggaaatggg
    50581 taaccgtctt gggttacgca acccagatgt gtttgtccct ttgtgggtcg tagatttccc
    50641 attattagag tgggatgaag aaagtaaacg ctactttgct atgcaccatc catttacagc
    50701 ccctgttgat gaagacgttc cgttgtttga tacagatccg ggaaaaatcc gtgcaaaagc
    50761 ctacgacctt gttatcaatg gggttgaaat tggcggaggt tctgttcgta tccatagtaa
    50821 agatttacag tcgaaaatgt ttaatgctat gggctatacg caagaagagg ttgattatca
    50881 gtttggcttc ctgctgaacg cttttagata tggtgcccct cctcatgctg gtttggctct
    50941 tggctttgac aggttgactt ctatgtttgc cggcctcgat agtatccgcg atgttattgc
    51001 attccccaaa aacaatgcag gccgtgatgt aatgctcgac gcaccttcgg ttatccgtga
    51061 tgaacagttg aaagagcttg gaattgctgt agttgcaaaa aaagaataag tgatttattg
    51121 tcatctaaaa caagaaagcc tggttcattc aattaccagg ctttcttgtt tttatcatct
    51181 tatttactaa cactgttatc cggtaaaata aaatagatcc ttataaatac tagtaatatc
    51241 gttgattaga aacagatcgc cccgtcgggg cttaatttta ctacaaacag gacgtcgcta
    51301 cgcgactaaa taatagcagc gtagcagcga actgtttgta gaataaccca atacaccaaa
    51361 tgttcctaag ctgcatagca gcgacctgtt ttttccggac aacaattgat ttactaattc
    51421 tcaaattagc tcatttcctc atttaccttg tgtgtctgtt cagcagcttt tctaccgggc
    51481 tgatttcaat aaacgaagca ccaaagttgc tgccatagtt gcctgcaata actactacaa
    51541 ggttatcttt ctcaagcatc ttttcttcaa ggcagtggga gagcgctttg tgaacaaatt
    51601 catagctgct gtttgattct tcaatgacat cagcaaaaac accaaatgaa aggctcaatt
    51661 cacgaactgt acgtttgtca tagcaaaaag caaatatagg tttacggcct ctgtaacctg
    51721 ccatattgcg gatagaacgg cctgtttcgc tatcagctat gatagctttg gcattgaggg
    51781 taatagccgc ttcaacggct gttttggtga ggtatgcaga gcgttgattg cttaatacta
    51841 ccattggtac atcatggatg tcgctacgtg atttttcaac ttcacaggca accctggcca
    51901 tggtttctac agcttgtaaa gggtatttgc cataagcagt ttcgccactg agcataatgg
    51961 catcagtttt actgaagata gcattggcaa tatcacttac ctcagcacgt gtgggccttg
    52021 ggttttctat catcgagtga agcatttgag ttgcgacaat aaccggcttg cggcgtgcaa
    52081 tacatttgct tacaatcatt ttctggatac cggggatttt ctcgtaagga atttcaatag
    52141 caaggtcgcc acgagctacc ataacgccat aaacgcaatc gagaatttca tcaaggttgt
    52201 caaccccttc ctggttctca atcttggcaa tgattttaat tttgctgttg tgtttgtcca
    52261 gtatttcttg gatggccata acgtcgttac ggttacggac aaacgagtgt gcaataaaat
    52321 caatatcttg ctcgatacaa tattttacaa aatcacggtc tttgtcgctc aacgaaggca
    52381 ggcggaaact tacagaaggg acgttgatac ttttgcgccc ttctacttta ccatcattgc
    52441 aaacttcaca aactaagtat tcttcatttt ttgatttcac acaaagttca agtgagccat
    52501 catcaatgag gatatggctt cctactggca cgtcatgtac aaagcctgta tagtttacgt
    52561 atatacattc cggtacagtt tctttttcag gttgtccggc aatttttact acatcgcctg
    52621 tttttactgc aaaccctttt gatggagtag ttcggatttc ggggccttta gtatctacca
    52681 ggatgccaat tttgtcagat acctggcgta caatgtcaat tactttttgc gcttgttcaa
    52741 atccctgatg ggctgtatta agacggacaa cgttcatccc agcctcatag agctggcgta
    52801 taaactcaac actacatccc ttgtcggata tggtggcgac aattttggtt tttttcatac
    52861 actaactaat cactttttct ttttcaactt taaaaaaact tcttaaaact gaacagaaaa
    52921 cttatatttt aaatactaac actaacacta acacactgac aatcaagtgg tataaaaaac
    52981 actttcggaa ggtgcaaagc tatgctgtat tactcgataa aaaaaattat ttttgaaaaa
    53041 aaagatgact tgggaagaag ctaatataag atttgtcaat tacctgaaaa tagagcgttc
    53101 attatcagct aattctgttg aagcttatga tgatgatttg cacaagcttc ggcaatattt
    53161 tgagtatatc aatgcatcag tagtgccaga gaaggttaca acaggacaaa ttcaggggtt
    53221 tttgcaatgg atcaatgatt taggcatggg cgcccgtagc caggcccgcg ttttgtctgg
    53281 cctgcgaatg ttttataaat atcttatttt tgacggagtt attgacaacg acccgatgga
    53341 actggttgaa tcgcccaaaa taggccgaaa gttgcctgta gtcttgtcta ttgaggagat
    53401 cgatgctctg atagcagcta ttgatttgag caaaccggaa gggaggcgga ataaagcaat
    53461 tattgaaaca ctttacagtt gcgggctccg tgtttcagaa ctgattgagc tgaggtatag
    53521 caacattttt tttgataagg gatttataaa agtacttgga aaaggcaata aagaacggtt
    53581 ggtgcctatt agcaacaaag ctgttaaaga aattcaacaa tatctgccag accggaacag
    53641 tatgcttgat gtaatgacta aagacagcga aaacatcctt tttttgaaca atcgcgggca
    53701 taaactttcc agggtgatga tttttactat tattaagaat ctggcagaaa aaataggact
    53761 aaaaaaaaat atcagtcctc acacttttcg gcattcattt gcttcgcatc tggttgaagg
    53821 aggcgctgat ttgcgagcag tacaagaaat gttgggtcac gaaagcattc ttactactga
    53881 gatatacaca cacctcgacc gtcagtacct tcgtgatacc atcattcagt ttcaccccag
    53941 gggataatta cagcatccat attaaataac ttcttatctt tgctaaaatt tttcaaacat
    54001 gatacttttc gttagcctgt ttttattggc ttatttattg ggttctattc caacatcagt
    54061 atgggtgggc aaatggtttt ttggtattga tgtacgtcag catggaagcg gtaatgccgg
    54121 agctacaaac accattagag tgcttggttg gaaagcagga gggccggttt ttgtagtcga
    54181 tattgcaaaa ggctttgctg ctgcatcgct tgtctgggct acagagctta tgcccaaaac
    54241 aagccagttt gtcaattttc agttaattct tggttttgcg gcattgcttg gtcatatatt
    54301 ccctgttttt gctggtttta gaggaggaaa gggggtagca actcttttag gtgtggtaat
    54361 agcattgtcc cctcttcctg cagtacttgc atttatggtg tttctggtaa ttctttttat
    54421 ttttaaatat gtatcattgg cttctatgac ggcaggttta agttatccat tgatgctttg
    54481 gtttttgttt cctaccttgc cgttgtcgtt aaaaatagcc gggattgtga tgtcgttagc
    54541 cttaattgcc acgcataaaa aaaacattct gcgtttgata aagggcgagg agtcaaaggc
    54601 ttattttctt atcggtaaaa agaataaata aatcatcatt ctggctttag tatgacggaa
    54661 caaaaaatac gctttgggtt agttttcttg cttgtcagca ttttctttgg tttaacagcc
    54721 ataggacagg gaattgttgt ggaacgttcg tcagagcgta tcatgataaa aggaaaggtc
    54781 tattttattc accaggtgaa gaaaagtgaa acattgtatt ctattgcaaa agcttatggc
    54841 attagccttg atacaatata tgcctacaat ccgggcacaa ctgaaggtat taagatagat
    54901 cagttgttgc gtatccctga ttataaaagt aaaggtactg tttatgagtc aaggcatgaa
    54961 gtggaaatac ctgaaaatat taaagaacca gcaaaggtac agcatactat tgttgcaggg
    55021 caaacattat acgggatagc acgtatttat gggctgaaac catcagatat tgaaaaagct
    55081 aatccgggaa tgatcagaga tagcctgaaa cccgggcagc agattattat tcctttggct
    55141 caattaaaga aagatagtgc cagtaaaaat tttttcatcc ataaagttca ggaaaaagaa
    55201 acattgtatt caatttcgcg attgtataag acttcggtag attctttaaa aataatcaat
    55261 ggcttatcca atgacagtat attgcgtttg ggcatggagc ttaaggttcc tatggttgtt
    55321 ggtgttgttc agcaaaaaga tatggcacaa acgtctgctg agacttctac gcctgtggta
    55381 atttcaatgg attgtgattc ttttcttgcc aaaaccgaac aaaaccaggt aaaccttaca
    55441 ttgttactgc cgttttttgc ttcaaaggca gagggagggg gcgaggatga aaatgttgct
    55501 gaagaaaaac gccacaatcg tcagaaatca atgtttagcc cacaatctgc ttcgtatgtt
    55561 gagttttatc aaggctgttt gttggcttta gaagatttga aaagaaaaaa aatagatgtt
    55621 aaccttcagg tttatgatac agaaaatgac actcttcttt tgcattcaat aatgaaaaaa
    55681 ccggagtttc aaagtgctaa tgtggttata ggccctgtat cattgtcgtt gatgaattat
    55741 gcttcacgcc acatgcaaaa aaatggtaca tattgggtat ctccttttgc tgatattaca
    55801 aatattacaa aaggaaatcc ttttgtattt caggtaaatg ctggtaaaga aaatttattt
    55861 cttgaaaata tcaaacatat tccggataat gacactaata aagttattgt tgtatataac
    55921 ggaagcgaat ctgatataaa tgatgatttt cgcaatgcag ctgcaaaatc aaaagatttg
    55981 aaagtagcgt caatgtggaa agagctgaat gtttaccaca atggttttgc tgaacttgtt
    56041 tcgatgcttg actctacaaa agaaaatatt atcatttctc cttctgtgga tgaaattttt
    56101 gtttctaatc tgttgagcat actcgaaagc aaattgttgc tatataggat taaggttatt
    56161 ggaaggactg aatggacctt gttttcaagt atagatatga attacttgtt tgaccagcag
    56221 ctatcttaca ataccccttt ttatatcgat tacgaaagcg aaccagtgaa aaggtttctc
    56281 aaaaaatatc gtttgtattt tggaactgaa cccgggcaaa tgtcaaagca aggatttaac
    56341 tatgctatgt taggctatga tgtaacatat tattttacaa tgggttatgc acaatatggc
    56401 aataaattgc cacttgctac tttgtgtttc aaccctgaga tgcttattac ccagtttagg
    56461 tttcaaaagg tttcacctgt tgggggaatg ataaattcta ctttacacaa agtaagctat
    56521 acaagggatt ataatgtgtt gcacgagtaa aaagtgttta tcctttcttt tttcttatca
    56581 aatcaacaat tccattgata atggttagtg ggtgtgctgg gtttcctggt atatataagt
    56641 ctatcgggta cttttcaagg aatgaccttt ccaatgccgg actatcagca taaatgccac
    56701 cactaatagc atcagttccg gcaaggataa ttactttagg gtcaggggtt gcattgtagc
    56761 agcgttcaag tggttctgcc atgttggccg aaatagggcc ggtaataagg atgccatctg
    56821 cgtgacgtgg tgaggcaaca aagtctatac caaagcggct aatatcaaac tgaacgttgt
    56881 tagaagcatt cagctcccat tcgcagctgt tgtctccggc ggcagatacc tggcgaagct
    56941 taaaggagcc actaaaaagc tgtttaatct cttttcgaat tattgattgg tcaagtgtta
    57001 cagggttctc atctccttct ttaactataa gcctttctct gacattggtt gctattttat
    57061 aatctgtagt aaaccttatt ttttgaggaa aagctaaagc acattctcca caaaacacac
    57121 atttaccaag gtctatgctt acagggttta aaccaatagc ttttaccggg cataaagcag
    57181 ttaacttatc ttcgtccact ttgatattgc tggcaataac aggacggcct ttaaaaatac
    57241 ccggtacttt ggcagttgta acatccggga tatattgttt cccgttgtgc cataatattt
    57301 tgatattttc taacatagtt ctcaggtttt ataagtcgtg tccgcaatag gacaagttga
    57361 aactcttgtt acaaatagga aaatcagata tctcgttatt tctgactgca agctcaaggg
    57421 ccttccagtt atgaaacgaa gggtctttta ttttatatac ttttagcatg cctttattgt
    57481 ctgtaatggc acaatggcat acttcaccac gccacccttc tgtaagcgag ataacaaaag
    57541 tatcagcttg ggcagtataa ttggaagcgt caactttctt aaattccggg atgttgttta
    57601 tccagttgcg tatgtagcca atagattgtc ttatttcttc ttttcgtatt tgtgtcctgg
    57661 aatatacatc tccatgacgt ttgacaacgg gttgatggtt catgtttttg tagttggcat
    57721 aagggtgcga catcctgatg tcgcgcttaa ggttgctcat gcgtgcagcc attccaacgg
    57781 ctccaatttg atatatctga tcattttcaa caactcctgt acgctccaaa cgtcctaagg
    57841 ctgaagggag cttgaatagc tgggttgcca tttcttcaaa gtctcgttca taaatagtaa
    57901 ggatttccaa aaaacgtttg cctaatgcct cggtgaatgg atagttaaaa ccaccaaccc
    57961 tgataagccc tttgcaaaga cggtttccac accactcttg gaaaaagttg accataggtg
    58021 tccttagtcg gccaaagact gcactaccca attggtaagc aatatctgta catatagcac
    58081 tcatatctcc tgtatgaata gctatacgtt caatttcttg tgctaaagaa cgtccaaatg
    58141 ctgtactttt gtcagtttca atattgcata ggctttccca gagctgtgca aaagcagttg
    58201 catgctctac agcactatct ccggcaatac tttcggcaat ggttatgcgt tcattcaggc
    58261 tttgtttttg caacataagt tgttcaactc ctctatgctg gtatccaagc tgtatttcga
    58321 ggtgtaagat ttgttcgcca tgacaaataa acctgaaatg cccgggttct attacccctg
    58381 catgtattgg gccaacacca acttcatgta attcttcgct actgcctttt aaaaaaggat
    58441 aattttcaat agtttgctgt ttgttggccc tgttaaaagg atatctgaca ggctttagcc
    58501 agggatgtcc ttcaaatttc accccaaagt tctcgtatac ttctctttca aacttatcaa
    58561 aagcaggaac atcaattgtt aatgatggat atgaagcgtc agtttcaaca tttgtggaca
    58621 atacattaat actttgctcc tggtcatcag caatacaaca aataagcttt atgttattat
    58681 tttcgcgata accaaaataa tttactatgt ggcaatgtat tttatccttt accaagctgg
    58741 tacagatgtc tgtaaactct ttgtaagtca gttcgggtat ggtttttaca ggaactggct
    58801 cgttgttttt aacagatata aacttgtttg tcatgcgatc agttttaaag atccgttaat
    58861 aagctcaatc aaaattgatg gagggcaata ggccatccaa atggcaagtc caagaagtat
    58921 gtattgtgaa agtgactccc atggcttgat tacaggtacc cgttcttcat taaagtcaat
    58981 tggtggggta aacaacagtt tgaatacgtt agacccaaag ccgtatatga taatggtgag
    59041 caatatgaac atcaatatta gtatcaggta tagtttttgg gctatcaatg actgaaatat
    59101 gagaaattca gatacaaaca tgcccgaagg ggggatggct gtcacagata agaagccaag
    59161 aaggacaact attgccccag ttttgttgta tttgaagtaa ttgccaatgt cgtaaatgct
    59221 tttactttga taaacccaat aaatttggtt gtattggaag aacatgacag gttttaccaa
    59281 ggtgtgtaaa acgatgtgaa gcagtgctgc atatacgcca atgcctccag cagcaatacc
    59341 tatcatcact atgcccatgt gttcaatccc cgaataggca aacatacgtt tgatgttttt
    59401 tatacgcatc atatatactg ctgctacaaa tatagataat actgcagcta ttaatatcac
    59461 tgtttttgcc cagtgttgta acggggtttt atttataagg gcgtatgttc tgtatatgct
    59521 tataaatcct acattcatca agatgcttgc aaataatgct ccggcaggtg caggggcttt
    59581 gtctttagcg tcaattccag ccgtgtacat tggcacaagg cctaatttag ctgtaaaacc
    59641 aataaatata aagatgaaag aaagcctgag ccagaacaaa tctgcttttg cataattggc
    59701 ataaagcgaa tcgaaagtta aatctgtaat ccccgcactc ttaaacgata aactcaggaa
    59761 taatatacca ataaacacga acgtaatgct gatagcacag ataaacacat atttccaaac
    59821 accttcaagc gcacggatat ttctgtgatg gtaaatgagc atagaagcgc taagtgtggt
    59881 tgcttcaaca aatatccagg ttaccgccag gtggttggaa agatatgcag cagcagatgc
    59941 tgtcattaag aatatgaggg ctgagaagaa caatcctttt gtccgtggtg tttcgccttc
    60001 attgtgcttt tccatgtacc tgtaaccatg aaataatgat ggaatggcaa caattgcaag
    60061 tgccaatagc aaaagaattc caatatggtc aggtttgaaa taagtaaagc tcgcttctgt
    60121 atgtatctgc aagtattggc ttatgccaaa tacccataat tgtatgacat acagcactaa
    60181 taatgaatac atcagcgatt tattcttgtt aaagaataga ccgatgccaa taagtgatga
    60241 taatataagg aatacagata tcataatgtt taatctttca ggtttcgtaa taagtccaca
    60301 tcagggtctt ttagcacatc gcctattttg ttgaagaaga tacccagtat taacacgctg
    60361 gcaaagatat caagcataat tccgaggttc accagcatag gcatctcatt accaactgcc
    60421 agcgagagga taaatacccc attttcaata atcaggtaac caataacgtg ggtgataatt
    60481 ttccggcgag atacaatcag gtaaaggcca gaaaagagtg tagataaggc tacaataaaa
    60541 aacgttttgt ctattagctt tgagcttaca gaacttgaca aaatgattga aattactatg
    60601 attaaagtaa caatgattaa tgaaagaaag ttgggaagga atggttcagc ttcacgtgta
    60661 atattattcc gctttatcag gctatttaga aaccaaggta cagcaatggc tttaaaaaca
    60721 atagtttcaa gcatgataaa tgctacattc aacggctcta ctttgtcaag cgtgagatag
    60781 gtgatagcaa acaacaaaat accctgaaat agaaggatat ggatgtaagt aatcatgcga
    60841 ttggctatac tcatgtaaag taatgacatg gcaagcgcta ttagtaatat atccgtcata
    60901 tctgttaaat taactttcct aaaacaagta aaactcctaa aaatattagc atagctactg
    60961 aagtgagtat aaatacaaac tgtgggttgt ggttcatcct gaaacgggcc ataaatgact
    61021 caaacaaacc gattataaat gcaaataaca cctggatgcc taaaaatatt ggaattgcaa
    61081 tggtaaatgg catggaagta atgaaaaagt tggcaatcag tgctccgtaa atggcaaact
    61141 tcagttgtgc cgagtaaaga ataaggccaa ggtcaaatcc actgttgtcc aaaatcatta
    61201 cttcgtgtat cattgtaagc tcaaggtgtg tctttgggtc atctattggc atgcgactgt
    61261 tttcaatcat tgcaataaac atcaatacaa aagccgccaa tatgccaatc atgtatgcat
    61321 tttcgcttct caaatgcagg ttgttaaaga tgtctgcaaa tgacgactgg cctgtaaaca
    61381 aggcaaaaga gcccatgaga ataaaaaatg caggctctgt aagcattgaa tatagggctt
    61441 cacgactcgc ccccattcct tcaaagctgc tcccggtatc gagcgaggag attatcatca
    61501 taaactttcc cagtgcaagc acataagcaa acatcacaaa atctccctga aaagacaata
    61561 ttcctttttc tccgccaaaa gggacaagca atgcagccat tatcaatgat gcaaagtaaa
    61621 tacttggcgc tatctgaaat acaaagctcg tggttttgct gtaaacagcg ccttttcgcc
    61681 acaatcgtac aatgtccttc ataggctgca gtattccggg gcctttacgt cccgatgcca
    61741 ggctcttggt tcgtattatg acgcccgaaa atacaatgct taccagcact atcaggatga
    61801 aactaatcat aatgctttgg tttttaaaaa gttcaacaaa gtgtctatat tttggtttat
    61861 tgatgtgtaa atcagtataa tcatgataaa taccaggcca taaagcaggt aaacctgtat
    61921 attgccgttt tgcaggaacg taaacctgtt taatgccagc cttaacaatt ttataggctt
    61981 atcaatcagc catatttcaa tgtaatcaag tatatgcgtt gaatgttcta tctttgaagg
    62041 atacaaaccc tttgcagcct tctttttctt tattgtttta aaaataaact cagctaactt
    62101 cctatattct ctgataaaag agctgcctga atattgcatg gctactgttt ctccggtata
    62161 accgcatccc caggtatgat cttctttttt gactttattt acaactatag cttttctgag
    62221 aatgattact gttgctatga ttaagataaa acaaatcgag aaccacccaa tatgggtagc
    62281 ttcgcgtaaa tacgtttcga tttcaggctg ataaatgaca aattgcgagg tatgtacttt
    62341 caatgatgaa ataagagaaa ataaggtttt gttcaataaa aatggtgcaa atccaatgat
    62401 agcaattgct atagctaata aaaacaaggg atatatcata tttccattgt ctgtattttt
    62461 gtattcaagc tttgtttcac gtttattgcc taaaaatatt attcctgttg ctttggtaaa
    62521 gcacatcaat gccaaaccgc ctatcaacgc catgatgata ataccaaaca atgcccaaaa
    62581 tgataagata aaataacccc cttgcaagcc attatacaac ccgacataca tcataaattc
    62641 ggatacaaaa ccgttaaatg gaggaattcc gcatattgca atagctccac tcatgaaaag
    62701 cattgtggtt tttggcaatt gtttgttcaa tccccctaaa cggtcaatat tcatagtatg
    62761 ggccgactga tatatgtttc cagaacaaaa gaataacaat gatttaaaga gtgcatggtt
    62821 ccaaacatgc agtaatgccc cgctgaaccc taatacaaaa agcaaaggca tgttgtaggc
    62881 tatgccaagg cagccaatgc ctataccaat gccaatcaga cctatatttt caatgctatg
    62941 ataggcgagc atctttttaa ggttgtgttg tactatggca agcataacac catatagccc
    63001 ggaaaccaat gaaagaaata aaatgatgta tcctgttatg caaaaatcag gatgcacaat
    63061 gaataacatg cgaataatgc caaaaatacc aatttttata accactccgg acataattcc
    63121 tgatatatgt gcaggagcag ccgggtgtgc aaccggaagc catacatgaa acggaaatat
    63181 acctgccttt atcccaaatc ctatgaaaaa taacccgtaa attataagct tggtgctgtt
    63241 aggtgctgat aacatatagc tggccatagc ttcaaaatcg taagaacctg tattagcttt
    63301 tccccaaaga aatgcgacaa aaagcattag caatcctata tgtgacacga taaaataata
    63361 cataccagct cgaagtactt cgcttttatg gctttcaaaa attaccaata cggtaatgct
    63421 tatggtcatt atttcccaaa caaaaagaaa aactaagctg ttttgtataa gacaaatatc
    63481 tattaatgat ataagaccaa gtatgaaagc tgcatagtgt agttttgttt ctgaagtcct
    63541 ttcggggtaa tgttttaaat atcccttact ataaaaatag cctatagtaa aaacgaaagc
    63601 aataatactt ataaaccaag ctgagagata atcagctttt agtgttatcg ggcctgtgat
    63661 aatattacca gaataagtaa aaatttgggt accgttatag aaaaccaata cagataaagc
    63721 aatagagaga gaaaagagta aacaaaaaac aacagatgtc atcattttat tagcttttga
    63781 gcccaccaaa gggatggcaa caactgctgc cagtcctatt atattgagta tttcaaagcc
    63841 aggtttaatc atttctttta ttttccttta tctgatgata ttccagaatg atcctatcaa
    63901 tataatcaac aaaaatacta ttccgtacaa caaatataat tgaacttgcc cgttttgtat
    63961 gaacctgaac aaagaaatca tacggttaac caaatttgta aagggttcta taatatacac
    64021 atcaaaaatg tcatggtagc gtatggaaaa tttcaatttt tgtatataaa taccctttct
    64081 ttctttaggt tctaaaagct tttgagaagg gataataggt aaaagttttg ccaatgtctc
    64141 ggaaaatgag cgtcctgtgt attgtgtagc tttagccggg ccggtatatc cgcatcccca
    64201 tgtttcgaag gttgtttctg gtaattttga ggttaaagcc tgtcttatgg caaaaacaag
    64261 cccgataatc aatataaaac aagcactaca tagtgctaca ttgcttaaaa tatgaggaga
    64321 acaagcagat atactattgg atatattgta tatagtggat agtttgttgg atgagtccga
    64381 aactaatgaa atataaaagg aaggaaaaaa agcgatgctc aacatagctg caataatgat
    64441 aaattgaggc aatagcattg cccagtttac ctcttttggt tggtgttcca gtttcttgcg
    64501 ggtattaccg aggaatatca aaccaaagtt tttagtaaat gtaaatatcg agatgcctcc
    64561 tgatatggcc agtacagcca tagataaaat aaaaagcatt gaaatttctg tattgctgta
    64621 gctcaaccct gcaatcattg atttgtaaag tacaaatttt gatacaaaac cgttaaaagg
    64681 tggaataccg catattgcaa ttcccccaat aagaaatatt gcagctgtat agggcataaa
    64741 tttaatcaaa ccgcctaaat gttcaatgtt ttttgagtga gtctgtctgt aaacgcttcc
    64801 tgcgctataa aaaagtagtg ttttaaataa cgaatgattc agaatatgca ataaagctgc
    64861 tgtaaaacct aaccatgata ttaaatgttc atttattcct tttccaatta atcctatacc
    64921 aattccaatg ccaataatac caatgttttc aatagtacaa taagctaaca tccgcttaaa
    64981 atcagagcgt aaaccagcat ttgctatgcc aaataacgtg gtgacaaggg acaaaacaag
    65041 tatgaaagtg ccaatggtcg tatattccat agggagatat aataccaccc tgataatacc
    65101 ataaataccg agcttgacaa gtaagcctga catcatcccc gatatatgcg aaggtgcagc
    65161 agggtgagct atggggagcc aggtgtgaaa cgggacaaaa ccagccttaa tagcaaagcc
    65221 cgcaaagaaa agcccaaaaa taacaagcac aatatgctta ttgttaagga gtgctatatt
    65281 gctaaagtct gaacttccgg tatttataaa taacagaatg aatgcactaa ttaagagtag
    65341 cacacccacg tgcatttgaa caagatagtt tagtgcagcc cttatgctaa ttcgtttttg
    65401 gtattcaaac attaccaata ccatcgatgt tagcgacatt acttcccatg ctattaaaaa
    65461 accaaaaccc tgctgaaata cacaaacgcc aatcatggaa agatgaaata acggtaaagt
    65521 catgtaatgt atccatttat ttgttggtag ttggtaactc ttcagataaa atataccata
    65581 aatactgcct tgcaacaccc caaaattgat aatcaacact aaccatgccg aaagtgaatc
    65641 caattgaata tttaaagtgc ccaaattatg cggcaataaa aacttttcaa ctattggata
    65701 acctgttatt gctgataaag aaggaataga agtgagtata atacccgtta gcaaagtcaa
    65761 aaataataga atatcctggc ctttattact taacaggccg gtaaataatg cattaattaa
    65821 tacaatgata aagcaaatag caataataga aataagttcc attggtcaat gttcgaaatg
    65881 tgacttttgt tatgaatttc aaaaaattgg ttagaagggg caaaattagt cgttataatt
    65941 tttaaaaagg catcaatttt tatgaaactt gttaactgtt atcgaaaaaa taattaacag
    66001 ttattgactt ttattaagtt tagttatata tttgaaaagc taattccaaa aaaggtttag
    66061 taaatttggg ttaatttagg gtagaggcag cttatatgaa aatatgagtt gcctttttta
    66121 ttcaaaaaaa ttataaactt tttgtcagag aatattgttg aaagatgaaa acgatttcta
    66181 aaaaacattt cttttgaaaa aacgtatatt tgcacagaat tttaaaaccc aaaataaaca
    66241 tggatattaa gcaagagttt gaaaaatttg ccaaagggaa aaaaggcatc agcagtctta
    66301 ccatgcaccg cttcgagtcg atgtacaaca gttatatatc gcctaccatt attgaggaac
    66361 gtcagttgaa cattgttaca atggacgttt tttcgcgttt gatgatggat cgtatcattt
    66421 tccttggctt gcctattgat gattacgtgg caaacatcat tcaggcccag ttgctttttc
    66481 tcgattcagt tgacagcggt aaagatatcc agatttattt caacactcct ggaggtggag
    66541 tctatgctgg tcttggtatt tacgatacaa tgcagtttgt aaatagcgat gtagctacta
    66601 tttgtacagg tatggcagct tcaatgggtg cagtattgct tacagccggt accaaaggta
    66661 aacgttctgc cctcaaacat tcgcgtatta tgatacatca gcctatggga ggcgctcagg
    66721 gacaggcttc tgatattgaa atcactgcac gtgagattca aaagttgaaa aaagaacttt
    66781 atgagatatt ggccgagcat tcgggcaatc caattaagaa gatagaaaaa gactcagacc
    66841 gagattattg gatgacagcc caggaagcca aagattatgg gatgatagat gaagtactgg
    66901 aacgaaaaaa aattaataca gtagatgaag gattctaaaa accactgttc attttgtggc
    66961 agaagtaaaa acgaagtaaa cctgcttatt actggtgctg aagctcaaat atgcgatatt
    67021 tgtgttggtc aggctggtga aattcttaga gatgaagtaa aagggcctaa gtcaaagttt
    67081 gatgtgtcaa aggtaaaact tttgaagcct attgaaataa aagccttttt agatcaatat
    67141 gttgttgggc aggaagaggc taagaagttt ttgtctgttg cagtttacaa tcattacaaa
    67201 cgtctcatac atgcaaataa aagagtggat aatgatgtag aaattgaaaa atctaatatc
    67261 cttttagttg gtcgtacagg aacaggcaaa actttgttag cccgcacaat tgccaaaatg
    67321 ttgcatgtac cttttactat tgttgatgct acggtactta ccgaagccgg atatgttggt
    67381 gaagatgtag aaagtatcct ttctcgcttg cttcagtcgg cagactacaa tgtagaagct
    67441 gctcagcgtg ggattgtttt tattgacgaa atagataaaa tagcccgtaa aggagacaac
    67501 ccatccataa cccgcgatgt ttcgggcgaa ggggtacaac agggattatt gaagttattg
    67561 gaaggcagtg tagttaatgt cccacctcaa ggtggacgta aacatcccga acaaaagctt
    67621 attgctgtgg atacacgtaa tattttgttc atttgtggtg gtgcttttga aggtattgaa
    67681 aataaaattg cccaacgcct gaatactaag gttgttggtt atactgcttc tgtcaagaag
    67741 accaggctgg ataaaaataa tttacttcaa tacgttgccc ctcaggattt aagggctttc
    67801 ggtttgattc ctgaaattgt aggtcgtttg cctattgtaa cttatttaaa tcctttagat
    67861 aaggccactc tgcgacgtat tcttaccgaa cctaaggatg ctattaccaa gcagtatgtc
    67921 aaactttttg aaatggacaa tataaagctc gtttttgatg aagaagcatt ggattatatt
    67981 gtggacaagg ctgtagagtt taagcttgga gccaggggct tgcgttctat ttgcgaagct
    68041 gtgatgattg atgctatgta cgaaatgcct tctgaagaac gtaaagaatt gcatattacc
    68101 ttggattacg cccgcaaaaa tattgaaaag gctaatttgg gcgagctaag ggctgcataa
    68161 tcctttcatc attggaaaaa acttttaaat atcaggtatt tggcctcaat attataagtg
    68221 aaattgagct gccaatacct gaaaaatcat ttgatactgc gcaagtagag attcgttttg
    68281 gtgaaacacc tttcacactt ggtggtgaag ccaggcgtgg tgttgtatat gaagcattgc
    68341 ccaatgcagt gctcattaaa gtgtttgata ttgccaggtt tcatattgtc gatggttctg
    68401 ctattattgt tcagccttta gaaaaatatg ataacaaatc ggcagttgca tttatcatgg
    68461 gttccgtatt tgcatctttg ctttatcagc ggggtatcat gccattgcat gccagtgcac
    68521 tggaatataa agggaaaata attgttatat gcggtgtgtc cggggcaggc aagtcaacac
    68581 ttgcacttgg tcttgctaaa caaggatttc gtattatttg cgatgatatt gttccgattt
    68641 attttcaaaa tggcttacct atggcaagtt ccggctttaa tcaaagcaaa atatggaaag
    68701 atactgctgc tataatggat atctctgttg aacctcttgt aaaagtgaga gaagagatag
    68761 ataagtttta ttatgaggca gaaaatgttt tgttaacaaa tgagccgtta tctgtaaagc
    68821 atatttttat tctttctgca accaataaag aaaatctgga aacaaaaaat attgaaggaa
    68881 aagataaatt tttggtttta cgaaatcatt tatttcgcaa acaaattgca ttctcaatgg
    68941 gtttagaagc caggttgttt ggcagccttg caaaattagc tgcttcagtg cctgtaacca
    69001 aaattgagcg gcctacaaaa ggtttttctt tagaaatacc ccaaaacagc attcttcaaa
    69061 tattagattt gtaaactatg ggtaatatta actggttggc atcttatcct aagtcaggca
    69121 atacctggat cagaattttt ctggcaaacc tgatggctaa taatgcgcaa cctgttaata
    69181 tcaatcagct acaggatttt tctattgcca gtaaccgtga gacatttgat gaattgtcgg
    69241 ggttatcatc ttcagacctt actttggaag agattgagcg tttgcgccct cttgtatatg
    69301 aaatccaatc tgacaatgct accgaaaaaa tttttttgaa aattcatgat gcgtatattt
    69361 atacttgtga tggtatcccg cttgtttcaa caagagcaac aaataaagcg ttgtatatta
    69421 tacgcaaccc tttagatgtg gcagtttcgt ttgctcatca tttgggtgct actcatgaaa
    69481 aggcggttga aaatatgtgc aatgaacatt ttgctttttg taacaaaaat ttaaagttgc
    69541 ataaacaatt agaacaaaag ctattgtctt gggaaaagca cgttttaagt tggacaagtc
    69601 agcaaaatat tcctgttaaa gttgtccgtt atgaagattt gttgcatttt cctgaaatta
    69661 cttttacttc aattgttgag tattttgagc tggattatgg caaaagccag attctttctg
    69721 caatacaaaa tagcagtttt tctgagttga agaaacagga ggaaactcac ggatttatcg
    69781 aaaaatcacc acaggcaaaa tcattttttc gtaaaggaaa ggccggactt ggcaaggatg
    69841 agcttggcgc tgaattatcc aataaaatca ttgccagtaa tgaaagggta atgcggcaat
    69901 ttgggtattt gtagaataaa aaaagccgga acattagccc cggctttttt tatagtctta
    69961 atttttataa cttatttgta ttcagcaaga aatttcacta cctgattgga tacgttgtca
    70021 gcagtaaatc cgaacttttc gtcaagtact tttgccggag ctgagtatcc aaaatggtca
    70081 aggccccata ctttaccttt ttcacctact aatccgagaa gagttaccgg caatccggct
    70141 gtcagcccaa ataccggaat cccggcagga ataacgcttt gttggtattc tttagtttga
    70201 gttctgaaca gtccttctga gattacagaa acaacgcgta cttttaagtt cttctgagct
    70261 ttaagcagcg cagcgccttc tactaatgta gctacttccg atccacttgc tacaagcact
    70321 acatctggtg tcccttcgca gttgacaaca atgtaggcac ctttttctgc ctgaagagct
    70381 tctttgtaac gcgagcctga tgcaggtaaa tccttaatat tttgacgtga aagaattaag
    70441 gctgttggag ttgctgtgtt ttccattgcc atcttccatg caacacttgt ttcataggca
    70501 tcagcaggac gaagggccaa caaactattc ttaccatggt ggtttttcaa ctgttccatc
    70561 aggcgcaact gagcttcatg ctcgattggt tgatgggttg ggccatcttc tccaacgcgg
    70621 aatgcatcgt gagtccatat atatttaact ggaagctgca tcaatgcaga caacctcact
    70681 gctggtttca tgtaatcgct aaaaacaaag aaggtagcac atgcagccca aacgccacca
    70741 tgaagtgcaa taccgttaca aattgctgcc attgtaagct cgctaacgcc agcttgtaag
    70801 aaagccccac tgaaatcacc tttggtgaag gccttggtat attttaaaaa gccatctgtt
    70861 ttgtcgctat tggcaaggtc agcagaagca acaatcatgt tttcaacatt ttgtgcaagg
    70921 taccctaaaa cagcagcaga agcagcccgg cttgcaatat tttctttttg cgcaatgctt
    70981 tcaaagttca actgtggcaa ttgacctgat aagaaaagtt taagttttgc agctttttca
    71041 ggatttgctt tttcccattc tgcttgttgt ttctttcttt ctgctgcttc ttttttcttt
    71101 tgttcaagag cattgctgta caattctttt acttcaggga aaattacaaa tggattcttt
    71161 ggatctccac ctaagctttc aattgatttt tcaaaagagg cacctgcttc gcttagtggc
    71221 atgccgtgtg tagcacagtt gcgctcgaaa ctcgacccgt cagctttcat agcgccttta
    71281 cccatgatgg ttttaccaat gataagggta ggtttttcgg tttctgcatt agctgctttt
    71341 aaagcttggc gaatttggtc agcattattt ccatcaatag taacaacttt ccagccccat
    71401 gcttcgtatt tttttgctgt atcttcaatg gttacagctt ttgtttctgt tgaaagttgt
    71461 atgtcgtttg aatcgtaaaa cataatgagg ttgctaagcc ctaagaaacc tgcaatacgc
    71521 cctgctgctt gagatatttc ttcctgaata cctccgtctg aaatgtaagc atatgttttg
    71581 tgggccatcc attcaccaaa acgggcaacg agaaaccttt cagcaattgc agctcctacg
    71641 cccattacgt gtccttgtcc aagaggcccg gaggtatttt ctacgcctct ttttacatca
    71701 acctcgggat gtccgggtgt tggactgccc cattgtctaa aattgcttac ttcatctaag
    71761 gtatagttac ctgcaaggca caaaacgcta taaagcattg gggacatgtg gcccggatca
    71821 aggaaaaacc tgtctcgcat atgccatgtc atgtcagatg ggtcatagcg taaaaattcg
    71881 gtgtaaagaa tatttataaa atcagcgcca cccatagctc ctccaggatg tcctgatttg
    71941 gccttttcga ccatagctgc agccaaaata cgaatattgt cggcggcttt gtttactact
    72001 gtgttattca tcgttttaat tttttgcgca aaggtactat tttcttcttt ttgttttcta
    72061 aatttataaa tgttttgttt acacttattt gaactaaaca tgaaaaaatc cgaaattact
    72121 gaatttcgga ttttttgctg aaagaaaaat cttactaaat tggttaattt gtatataagc
    72181 tatgcaggtg tagtccgact ttatatctgc atttcaaaag ccgataaagg ctgatggaat
    72241 ttattctgtc acttcaaatg aaccctctaa accttcgctg ctattgggtg ctatccaaac
    72301 cttaaattgc ccgggttcca caacttgttc catttttgca ttccaaaatt taagctggtt
    72361 tgtgtttaat gtaaaagtta tttttacagt ttctcccggt tttatggcta cccgtttaaa
    72421 atctttaagc tcttttaccg gcctggttaa cgaaccaact aagtcgcgga tatacagttg
    72481 aacaatttct gtagcttcta cgttgcctgt atttgttatt ttacagctaa tttccatttg
    72541 gtcttctttt ttcatttgat ttttagaaag cttcaaatct gaataaacaa atgtagtata
    72601 gcttaaccca tagccaaatg gataaagagg tttgtaacct gcatcaagat aatagcttga
    72661 gcagcctaaa gtaaactgtt ttgcactgac agggatatcg ttgagtaaca tcaatggttc
    72721 tttggccggg cgccccgaat ttttttgtga ataataaacc ggtatttgtc ctgtcatctg
    72781 aggaaaggtt actggcaatt tgcctgatgg cggcactttt ccaaatagta cgtctgcaat
    72841 tgccggtcct cccatagttc cggggtgcca tgcatagaca attgcatctg ctattttact
    72901 ttcttcagtt atagcgagcg gacgtccggc catgataacc aataccaatg gcttgccagt
    72961 ctttttcagc tctgctatca gttctctctg ttttcctggg aatgtaatgt ctgcacggca
    73021 ccgggcttca ccggaaagta tagattcttc acctgcaaac ataagtatta catcagattg
    73081 acgtgcagct gaaatagcag cagtaaagcc ttttgtgctt aggtcacggc tatattccaa
    73141 tcctttggca tagttgactt ttacttgttt gccaaactct tctttaattg caacaagagg
    73201 ggttatagta tgttcttgtt ctccgtctaa tgtccatgtt cccatttgat cgtatggtgc
    73261 atctgccaat gggcctatga tagctactga ttttacagtg cttttaatag gtaatgtatt
    73321 gttttcattt ttcaataaaa ctaaactctg gatggctgct tctttggcac tggcaaggtg
    73381 ttcgtcagag tataagcttg acttgctgtt gacagctaca taaggcttat caaacaatcc
    73441 aagtctgaat ttgaggcgaa ggatgttccg taccgcttcg tcaatagtac tttctttaat
    73501 cttaccttct ttaatcaggt tttcaacgtg ttcaacatag gtgctggtta ccatttccat
    73561 ttctactcct gcgttaattg ctttcatcgc tgcttcttta cggtcagcgc agaaaccgtg
    73621 attgatcatt tcttctactg agccccagtc gctaactacc atgccatcaa aattccattc
    73681 tttccgcaat acctgtctca aaagaaaatc attacctgat gaagggaccc cgtttatttc
    73741 gttaaacgaa accattaacg ttgccgctcc tgcatctatt gatgctttga atgatggaag
    73801 gtaaacttcc cgcaatgttt gttcgggtat tatggtggtg ttgtagtctc tgccgccttc
    73861 tgtagctcca tatccgcaga aatgtttgac acaggcagca aggctgtttg tgtcattcag
    73921 gcttttcccc tgaaaccctt ttaccattgc agctccaagt acacttgtta ataccgggtc
    73981 ttctcctaat gactctgcta tgcggcccca acgtgggtca cgcgtaatat ctatcattgg
    74041 ggcaaatgtc caccttattc ctgcttcaga tgcttcctga gctgctactc ttgctccttt
    74101 ctcaattata tcagggttcc acgatgccgc ctgacccaga ggtatcggaa atatggtttt
    74161 gtagccgtgg ataacatcgc gggagaatag cagaggtatt ttcaaacggc tttcttccat
    74221 gactaccttt tgcattttgt tgatggttac agggttcacc tcattaagga atgagcccac
    74281 catccctttt tttagcatct ctttgaagtc gtctgcattt ccgcttgaac tgagttggtt
    74341 catctgtcca attttttcgg acagggtcat tttcgaaagc aaatcttcga ctttgttttc
    74401 tatttcgcta tcttgtcctg tttgttgctg ttggcaagat actattaaaa tggccgtagc
    74461 cattaatatt agttttttta tcatagtcaa aattgtttgt gtttgttaat ttttgccaaa
    74521 aataggatgc aggagaatga aaatactatt tttgagctac tataaagtta atacaatcct
    74581 acgattattt tacggcaaca aattataaat atcaataatc cagcaaaata catttatggt
    74641 tttaaaaatc taaaagttcc ttttaattaa atgttgtttt ttattcacct ttgtcaacta
    74701 aacattattt ttactctcga aatttgacct gatgaatgct gttttgcgaa ttatattttt
    74761 tgtttctctt gttatagtcg gtttttctgc ttgtactgtc agagaggtta tatctgtcag
    74821 tcatagtgca agggataatg ttactattga cggcattgcc ttgggtacag cttcatattc
    74881 aaagttattt ggcgttgaaa cttcttctgc tgatgcttgt gtgttgcaag caaggttggc
    74941 catgtacagg gctttcccgc tacagcccaa ccaagcattt gacaacatgg tagtggagaa
    75001 gaaaaccact tatttcccat tttactctaa aacaagattt ttgatttctg cagacattat
    75061 tactcccgaa attgatagta aaaatagatt tgcttattct ccttcttttg aaaatatgct
    75121 tttttctaac aaaaagattg aacatgaagg cttgcaattg gctgattctg tagtttttaa
    75181 atataaaggg aaatatacaa aaggtgttat tctggatttt tcagataaat cagtgtggct
    75241 gggcttaaat tttaaaaacc agctgcgaat agtcaaggct tcggtaaaca aagtttttgc
    75301 catcggaaat gaagaacttg aagatgaact tgggcttgag actggtcaaa ttattgaaat
    75361 ctcttacgtt tcgactaata ataaccagga tgtttgctcg aaagaaattg ttggcatgaa
    75421 taaataccac gtgcttattt tacagaaaaa cgggactgat tattcatacc aggtggttgc
    75481 agctaataaa attaaagaaa aaaagcatta aagcttttac tctttaaaaa tgggttttgc
    75541 atataactct acgatatttt catcaatttc attgtcgctg aaaataatga gcctttccat
    75601 gatattacgg aattcacgga tgtttccggt ccaatttatc tgctgaagct tgctgatagc
    75661 ttgttctgta attctttttg gacaacgtcc atattcattg catattagct gattgaaatg
    75721 ttctgcaagc agcgggatat catctttacg ttcgtttaac gaagggacct caataactat
    75781 gacacttagc ctgtggtata aatcttgtct gaacctgttg gcggctattt cagatttcat
    75841 atctttattg gtggctgcaa gcacgcgaac atttactttt atctcttttt caccgcctac
    75901 tcttactatt ttgttttcct gaagcgcacg taaaaccttg gcttgagctt caaggctcat
    75961 atccccgatt tcgtccagaa acaaggtacc tccgtctgcc aattcaaatt tcccacgacg
    76021 gtctttgatg gcagaggtaa aggccccttt ctcatgacca aaaagctcac tttcaatgag
    76081 ctctgaaggt attgcagcac agttaacttc aataaacggg gctttactac ggttgctaag
    76141 ctcatgaatg cgccttgcaa taagctcttt accacttccg ttgggcccgg ttatcaatac
    76201 ccgggcttct gtggcagcaa ctttgtcggc catgctgagt acattcatga tagcatctga
    76261 ctttccaatg atctcatacc ctttctcaac tttcttttta aggaccttgg tattggtaat
    76321 gagatgagtt ttgtccaaag cgttttttac cgtgattaaa agcctgttga ggtcaagagg
    76381 tttctcaata aaatcaaatg cccctttctt tatagcttct acagctgttt caatattgcc
    76441 atgacccgaa atcataacaa tctgaacatc tccggctttt tcaaatattt tctcgatgag
    76501 ctcaagacca tccatcttgg gcatttttac atcgcaaagg ataaggtcaa attctttcct
    76561 ttcaagtatt tcaagggcca ttagcccatc ttctgctgtt tcaacagtat gtttttcgta
    76621 ttcaagaatg tctctcagcg tgtttcttat tccacgttca tcgtcaatga ctaaaatgtt
    76681 cgccattttt tattttgata tgattaaata gtttgacctt aaagttgtct gatattgcat
    76741 cagaggcttg tatgctttgc ttatattggt aggggctcca tctgtgatga gcataaattc
    76801 agacccacaa caaggacaga ctcctttcca tcctgcacta tctatgctta gcttacagta
    76861 gtcatcataa gccctgtaag tgcaggtggc atcaaatgcc tgaaaatcat caatgcccct
    76921 ttgataaatt aaaactccat taccataagt tttgtaagta attctttttt tagggaattt
    76981 agggttatga taatcaataa tagtatagtt ttcatttata ctgtccggat aaaccgaaac
    77041 aaccatgtct gtcccaagat aagctaaatc agaagtaagg cttaattcaa tatatacagg
    77101 cacttcaggt acaacaggag ttgtatctgt gtcgcaaccg atataagtaa ttaaaagtaa
    77161 acttattata aatatttttc ctaattttga tgaaataaac ctcttagcca tgttcagatt
    77221 ctttggctcc aaaattaaac tttctgtgct gaaattgata attttcattt tcctatttca
    77281 attcccttta catttttatg ctcaggaaca aacaaaagac aaaagacaag agcaaatgga
    77341 gaaaaaaaaa acaagaaaaa gagaaaaaag aacaagaaca ataagccaaa gctcaacaga
    77401 accaaattaa tattcaaaac aaaaaaac
//
LOCUS       DAOSPF010000008        71251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_397_length_71251_cov_6.894053, whole genome shotgun sequence.
ACCESSION   DAOSPF010000008 DAOSPF010000000
VERSION     DAOSPF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 71251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_71251_cov_6.894053"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PK252_02820"
     CDS             complement(<1..1037)
                     /locus_tag="PK252_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT13672.1"
                     /translation="MYDYLKELSVSLEKLILSHHLSPTVAHSLNLMANIVAIALMAML
                     ANFVAKKIIISIIKKWVDKSTNDYDDVFYEKGVFNRLSHLAPALVISKLTPIALYGYN
                     VAINTIATLTNIYILLAVIAVIMAVINALHDIYNMTPMGKQRSIKGYIQAAKSIVIFI
                     GVLMVISILVKKDLSSLFAGLTAFAAVLMFVFKDAILGLVAGIQISSNDILRVGDYIE
                     MPSRGADGTVIDIKLSTVKVHNANRTIVTIPTYSFVNESFLNWRGLDISLGRRIKRSI
                     NIDMQSIFFCNDQQLEEFKKNPLVERAIEKSSPGIYDKITNAQVFRLYIEEWLKNNKD
                     INAGMPFLVRYL"
     gene            complement(1127..1837)
                     /locus_tag="PK252_02825"
     CDS             complement(1127..1837)
                     /locus_tag="PK252_02825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT13673.1"
                     /translation="MKVKFLVSILAISLVVLSACNKKSTVKLRNDVDSASYAIGVSIG
                     ANLQPLDMPAFNAELFKQAVMEAMAKKEHKMTQEQIQMFLQQYFTNLQKKQAEASLKK
                     NEEFLKKNKERKGVITTASGLQYEVLREGTGATPKAEDMVTINYKGTLIDGTVFDSSY
                     ERKEPITFAVNGVIPGFSEALQLMKVGSNYKIYIPSQLGYGPNPQGKIKPNSLLIFEV
                     ELLSISTPEQKKEEAKKQ"
     gene            complement(1864..2562)
                     /locus_tag="PK252_02830"
     CDS             complement(1864..2562)
                     /locus_tag="PK252_02830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT13674.1"
                     /translation="MKKILTFIFIGSIAFNAFSQKKQKEELKTNLDSVSYCLGTYFGN
                     SLKAAGVSAINNEAFIKGLEAELSGTSKISQEVANQLIQAFMMKQFQAKTQKNLAEGR
                     KFLEENKKKEGVITLPSGLQYKVIANGEGESPTINDKVTCHYEGKLINGKVFDSSIQR
                     GKPMQFAVNGVISGWTEALQLMKPGAKWILYIPSELAYGEQIMRGSEIEPNSVLIFDI
                     ELISVEKQTPAENK"
     gene            complement(2658..5777)
                     /locus_tag="PK252_02835"
     CDS             complement(2658..5777)
                     /locus_tag="PK252_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT13675.1"
                     /translation="MLNKYSFKSNIYKGFIVFIGSYLLLAQHGYGQYFNGPNKPDYKI
                     FEYKVLTSPHFELYHYLENDSVKNAFMAASEKWYERHFMIFSDTFKEKNPVILYANHA
                     DFQQTNAVSGLIGVGTGGVTESLKNRVIMPLMESAAQTDHVLGHELVHAFQYNFLLSD
                     DSLQLRNMRNIPLWMIEGMAEYLSIGSTDVLTAMWMRDAILNNDFPTLDDMSRSYKYF
                     PYRYGQAFWAFVTGYYGDTIIKPLFDLTTKYGYDKALETLTIWNERSFSQAWKNSMQQ
                     YYSKFIAKADKQPTGTKILHRKNSGAINIAPSLSPDGKYVIFLSEKDLFTFDLFMADA
                     KTGKIVKKISSTIRQNQIDDFNFLESGGTWSPDGKKFAFVTFSKGRNRLLIIDINNPA
                     YSTEKDLKTIDAFSNPAWSPDGKSIVVTGLKEGVTDLYSYQVEKDTTFRLTNDVYCNL
                     MPSWSKDGRFIVYSTDRPAEGQTGIQKGYSLAIFDVQTKEIKVLPVFPGAENLNAQID
                     STGDNVYFLSDANGIRNLYHYNVSSNETKRLSNAQTGVCGVTHFTPALTMNHQNIIYT
                     YYWNGDYNLYKANINDFKAVKVNTDEVNFDAAILPPFKRIGKDIVNVNLGEAIDKSTL
                     PKDSIIPVPYRSKFQLDYIGGSNVGVSAGSYYGTGMSGSVDMLFGDVVGNYQLYTSLA
                     INGQIQDFGGSVAFINNKNKIDWGASLSHIPYKYGNYYYSNDSVDDNLVSVLNVDMQW
                     IFENSIALFAIKPLSQTRRIEASISYSYYNFYLERDKYIGSIYGYSTWIGREKNLPTD
                     KGFGIASVSTAYTLDNSYMGIASPLRGQRFRVEVDQYVGKINFTNLLIDYRKYFFINP
                     YSFAFRLYHQGRYGNVTTNNVIRSLYLGWPWLVRGFYGFIGADSYLANQTMLFGSRLA
                     IANAEFRIPFTGPERLCLIPFKYFVSELSFFTDAGVAWSKIDQLTLNRHKNETLKVNY
                     KNLATSGPDNMFDGLDENYYRYPLMTYGVSLRINLFGVLIIEPYYSIPFMKDWQSYAG
                     LNLNLFPGW"
     gene            complement(5813..6493)
                     /locus_tag="PK252_02840"
     CDS             complement(5813..6493)
                     /locus_tag="PK252_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT13676.1"
                     /translation="MNSTLCHQCYSGCPFYLCIKDTGQGLLLNEVSEIFYKRKELIFK
                     QNTRSTQIFYLKSGLVKLSIERRNEKNAILKILTPGSFIGINSFSKDLCQYSAAALTN
                     SEVCIIPKEVVFKLITSNPCFAQIIFDAISEKYAYIIRKISSLSTKQMHGRLADVLLY
                     LNSSEFNNTDVFSHITRQDIAELSGMALESVIRLLSEFKEDGLIRLDGKKIVINNEEL
                     ILKLSEIG"
     gene            6672..7712
                     /locus_tag="PK252_02845"
     CDS             6672..7712
                     /locus_tag="PK252_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT13677.1"
                     /translation="MINTLKNISFHIQGFLLFFDIDCTLVFCNDDSFVPGLIGKKIGE
                     HEISSEKKLLINKNRKVCTVPQTATVNIENVRYETALFTCENGENETSGYILIVRAQK
                     ENRNGEDHYFDDVSNLFLSNIAHEIRTPLNGIIGFAELLSKKDFPVGKIREYAQIIHS
                     NGNYLFKLITDLLDLSRIEAGKLTLFKSQFSLNRLLYDLQLFFMLDMKNRNKSHISFK
                     LSLELPDGSDFITADELRIKQVLINLIGNAIKFTEQGTITVGYKLATKDMLEFFVQDS
                     GIGMPSSMTEKIFGRFVQANDKIAFTHGGTGLGLAIAKEFVELHGGKIWVESKEGCGA
                     TFYFTLPLFFPS"
     gene            8043..11576
                     /gene="nifJ"
                     /locus_tag="PK252_02850"
     CDS             8043..11576
                     /gene="nifJ"
                     /locus_tag="PK252_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT13678.1"
                     /translation="MADKKFVTCDGNYAAAHVAYMFSEVAAIYPITPSSNMAENVDEW
                     AAAGRKNIFGQTVRVVEMQSEAGAAGAVHGSLQAGALTSTFTASQGLLLMIPNMYKIS
                     GELLPGVFHVSARALAAQALSIFGDHSDVMSARQTGFAMLATGSVQEVMDIAPVAHLT
                     AIKSRVPFLHFFDGFRTSHEIQKVEAANQEELVKLLDQKALQEFRERALNPEHPVTRG
                     TAQNPDIYFQTREAANKFYAAVPEMVESYLGEIKKITGREYHLFDYYGAADAENVIIA
                     MGSVTETIKEVIDYLATKGEKIGLISVHLYRPFSAKHFLATLPKSVKRICVLDRTKEP
                     GANGDPLYLDVKEVLYGTANAPMVIGGRYGLGSKDVTPGQIIAIVKNLKANEPKNQFT
                     VGIVDDVTFKSLPVEPEVNVIKAGTFEAKFYGLGSDGTVGANKNSIKIIGSTTDKYSQ
                     AYFAYDSKKSGGITTSHLRFGDTPIRAPYLVVSPDFVACHVPSYLDKYDMLAGLKKGG
                     TFLLNSIWDAEETKKRLPDHMKKQLADAEAKFYIINATAIAEEIGLGTRTNTIMQSAF
                     FKISGVIPYEIAVCEMKKAIDKSFGKKGEEVVKMNYAAVDRGGAVIEVPVPAEWKKLD
                     GTVTIGKTSAKVPDFIKNIVVPMNAMKGDDLPVSAFNNREDGTFDAGTSAFEKRGIAV
                     NVPEWQAENCIQCNQCSYVCPHACIRPFLFNEKEAAGMPAGSKMIKANGKALEGLQYK
                     IQVSPLDCTGCGNCADVCPAKTKALVMKPLETQQAEIARWEYVAKNVTYKDNLMPKDA
                     AVKNTQFAQPLFEFSGACAGCGETPYIKLITQLFGDRMMVANATGCSSIYGGSAPATP
                     YTTNSKGHGVAWANSLFEDNAEYGLGLATGVRQIRERIALKMNENMEVVNAATKEAFA
                     EWIAGKDNAEASITASEKVRAALKNEKAPIAAEIAKLDQYLVKKSVWAFGGDGWAYDI
                     GYGGLDHVLASGEDINILVLDTEVYSNTGGQASKSTPVGAIAKFAASGKRIRKKDLGM
                     MAASYGYVYVAQVSMGASQAQVLKAIREAESFPGPSIIIAYAPCINHGLRLGMGKSQN
                     QAKLAVECGYWQLYRYDPRLEEQGQNPFQLDSKEPKWDEFQNFIMSEVRYSSLKKAFP
                     AEAEELFKAAEENAKWRYKTYLRQSTLDYTK"
     gene            complement(11668..12150)
                     /locus_tag="PK252_02855"
     CDS             complement(11668..12150)
                     /locus_tag="PK252_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13679.1"
                     /translation="MSINNKNQIAGYVLASLMLLTLLNSAYFFLSIVKPDFFRWLAFN
                     ACSLAIIAYLTCFVCYKITKKDFFLAIAFVPMYYYGTMGLFVVSWEAANLFAQITHII
                     ITLNAIWVLFELLKESKYDSLGKGLLIGLLVFVPVFAVIQVFSQQHMAEFIRMLQKVH
                     "
     gene            12563..14299
                     /locus_tag="PK252_02860"
     CDS             12563..14299
                     /locus_tag="PK252_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13680.1"
                     /translation="MLVLLLLNTYFALCSSAQTIPLVYDVENTGDTFAKPVLPTKDQL
                     PVVEPLTDPFMWSDGSGRSTNFSDWSHRRAEIAAEIQHYEIGAKPKRPDTITATYSGG
                     VLTVVVTENGKTLTLKSQVVLPGGTGPFPAVIGMNSGTGSLPPSLFTNNGIATITYNH
                     NDVTTYNGKSATNPYFQMYPDLIYAGQYSAWAWGVSRLIDGLELVQNTLNIDLKHLAV
                     TGCSYAGKMALFAGALDERIALTIPQESGGGGAAAWRVSQSLTESVETLSATNYSWFM
                     QSMGQFGGASVAKLPHDHHELLALVAPRALLVLGNNDMIWLADESGYVSCRAAQKVYE
                     TFGIADRFGFSIVGGHGHCALPSGQYPEVLAFIKKFLLGIDTINTNVRISPYQYVDYA
                     RWTEWWGTGVAEFPVIEGEKLYEAECAIVGNNWESVSYSKCSNKTYLKAKTGISSTFV
                     APTDSANIVFVPITIDKDSTYYIFAHVNCPSGNADSYWVKVDDSSFVSVDGLSTSDWK
                     WRYLNSYKLTAGQHTLAIGINENGLCLIKYPSPHNHFSHQAWAKQLSIIVGIMNFFIL
                     SLNVPQKVRIGE"
     gene            14209..14985
                     /locus_tag="PK252_02865"
     CDS             14209..14985
                     /locus_tag="PK252_02865"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13681.1"
                     /translation="MGKTAVNNCGYNEFFYIEPECATKGKDWRVGYDTLASNNGYIVV
                     RPDTESVAAAPATKAGEVLLKFNPTQEAIYYVYGRVNCPSANDDSYWVKFDNEDYQMV
                     NGLTTSGWEWKVLASDTLTAGTHNFTIAYRENGAKLDKVCITNSSIVPSGMGDKASGS
                     CKVTDIEMVNIDGYSLGQNYPNPVIGQTNIAFEIPKSAFVSLKVFNLLGVQIAELAGK
                     TYPQGKHVVSFDAHGIPNGMYYYTIMANEYTCRKMMLKQK"
     gene            15218..15856
                     /locus_tag="PK252_02870"
     CDS             15218..15856
                     /locus_tag="PK252_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOT13682.1"
                     /translation="MEWFHWIELFIKGLVLGYITSIPLGPIGIIVVQRTLSKGKLSGF
                     VSGLGAASVDTFLAVIAGLGLSFIIDFIEDYSILFKLFGGLIIIFIGGRIFFKSPVRQ
                     VRERKQKKSSLHTDYLSVVALTLSNPLAVFLFIAIFAGLNVVNGVKSFVSLGFVFTGI
                     FIGASLWWLTLTSFVNIYRDKFRLKNLWWLNKITGVLIVILGLATLISILFN"
     gene            15867..16847
                     /locus_tag="PK252_02875"
     CDS             15867..16847
                     /locus_tag="PK252_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOT13683.1"
                     /translation="MNQLYPIRFRPILKDMIWGGSKLRKIFGKPGATDKSGESWEISD
                     VKNNSSVVENGFLAGNTLGEIAEIYMGDLLGDKVYERFGQQFPLLIKYIDANDKLSIQ
                     VHPDDKMAYEKHKSYGKSEMWYVVQAEPGAKLIVGFNKTIDADTCLQHLNSNTLPEIL
                     NFEDVKAGDVFYLPAGRIHAIGEGLLIAEIQQTSDITYRIFDWNRTDAQGKPRELHTE
                     LALQAIDFSPVKDFKTSYKNKLNHTVELVKCEYFTTNLIELDQTLEKDYESIDSFVIY
                     MCIEGQVIVKPYDQDSITIKAGETILIPAVLKNIIIEPLTTTKILEVYID"
     gene            16857..17510
                     /gene="yihA"
                     /locus_tag="PK252_02880"
     CDS             16857..17510
                     /gene="yihA"
                     /locus_tag="PK252_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT13684.1"
                     /translation="MSIKITSLNVEFFKSCPSLKGCPEGQYPEFAFIGRSNVGKSSLI
                     NMLANNRKLAHTSGQPGKTKLINYYLATFKEQVGNEENIRKIPWFLVDLPGYGFAKTS
                     KAQRGEFSSIISDYVFKRQNLTCLFVLIDSRLEPQQIDIKFITLLGQQGVPFCLVFTK
                     IDKISSNSVNSNIEKFKATLLEQWEEFPKIFLTSAEKKQGREELLNFVKDTIRSLKS"
     gene            17630..18568
                     /locus_tag="PK252_02885"
     CDS             17630..18568
                     /locus_tag="PK252_02885"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT13685.1"
                     /translation="MDRRSPLKFFSGTASTYLAEKISKSYGTQLGSTQLLRFSDGEFQ
                     PSYLESVRGCTVFIIQSTFPPAENLLELLLMIDAAKRASAKRVVAVIPYFGFARQDRK
                     DRPRSAIGAKLVANLLTAAGIDRVMTMDLHADQLQGFFDVPVDHLYASSIFVPYINSL
                     NLENLVVAAPDMGGSKRANAYSRFLKAEMVICYKLRKKANTIEEFKVIGDVRDKNIIV
                     IDDMIDTAGTITVAANMMMEEGARSVRVVATHPVLSGPAYERIENSKITEVMVTDTIP
                     LKQQSPKIKVISIADIFADTINKVYNYQSISGNFIM"
     gene            18688..19260
                     /locus_tag="PK252_02890"
     CDS             18688..19260
                     /locus_tag="PK252_02890"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOT13686.1"
                     /translation="MKVFELEGTIRPELGKKSAANLRKQDSVPCVLYGGKENVNFFVT
                     NPAIRDLIYTPNVYIVNLKLGKITKQAVMREIQFHPVTDKVLHVDFYEIADDKEVAME
                     IPIRIVGNSVGVRKGGKIKQAKRMLKVKALPKNLPDILEVDITNLDVEQTIKVADLKY
                     ENLVMLNGLREPVVSVMASRATAKAEAAAK"
     gene            19281..19841
                     /gene="pth"
                     /locus_tag="PK252_02895"
     CDS             19281..19841
                     /gene="pth"
                     /locus_tag="PK252_02895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT13687.1"
                     /translation="MKYLVAGLGNIGSEYAHTRHNIGFDVLDALAGASNIYFEDGRYA
                     FNTRFSIKGRTIVLIKPTTYMNLSGKAVSYWLQKENIPVENLLVVVDDLALPVGAIRI
                     RPKGGDGGHNGLKNIQEMLGHANYARLRFGIGSEFAKGRQVDFVLGKWAEEEKALIKP
                     AIETSCEVIKSFVLAGLERTMTSFNK"
     gene            19896..20210
                     /locus_tag="PK252_02900"
     CDS             19896..20210
                     /locus_tag="PK252_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13688.1"
                     /translation="MMKIIGPFKVLLIVALICADFLKAQDSLNYVKPPKKWGLGIVPA
                     VAYNTDWGFQYGVAGNLYNFGKTYPDYKYTYYFEWSRTTRGNSVNEIFFDQKFFLLKI
                     FD"
     gene            20207..21211
                     /locus_tag="PK252_02905"
     CDS             20207..21211
                     /locus_tag="PK252_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT13689.1"
                     /translation="MTYNVGYFFNKQLPFYGFDGYNARYLKENEDQNNTTFASRMYYR
                     LDRAVIRCLLDFQYKTAYNPLRILAGVGYFDTRIKPVDIDKFNESLDDDDKILKNTTL
                     FEKYVSNGIISEDEKDGGKCFILKSGVIYDTRDNETNANRGIWAEVLLISAPKPGNNT
                     SFTQVGAVFRHYFTISAPRLTMANRIGIQTLVAGQKPFYMLPYLFSSYRLRDAFGGEK
                     TIRGVLAQRIQGNGVAFGNFELRYKFLYGRLFKQETYLCANVFADWGMVTSKYLDKPV
                     PMALENEIKPGQEKPHWTTGAGLRVVMNQNFVVTADYGRALDLQDGKNALYITLGFLY
                     "
     gene            complement(21275..21592)
                     /gene="trxA"
                     /locus_tag="PK252_02910"
     CDS             complement(21275..21592)
                     /gene="trxA"
                     /locus_tag="PK252_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT13690.1"
                     /translation="MALEITEGNFEQVVLKSDKPVMVDFWAEWCGPCRMVGPIVEEIG
                     HEMADKIVVGKVDVDNNNAIAMKYSIRNIPTILFFKNGEVVDKQVGAVPKSTLVNKIN
                     ALL"
     gene            complement(21620..25228)
                     /gene="dnaE"
                     /locus_tag="PK252_02915"
     CDS             complement(21620..25228)
                     /gene="dnaE"
                     /locus_tag="PK252_02915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT13691.1"
                     /translation="MNFSHLHVHTQFSLLDGAAEIKRLYKKAMDDGQPALAITDHGNM
                     FGVFEFVAEAYKHKVNPDDKTDKRIKVKPIVGCEFYLVENRFKRQFTREEKDIRYHQV
                     LLAKNEQGYRNLSKLCSLGYIEGLYGKYPRIDKELIEKYHEGLIATTCCLGASVPKTI
                     LKRGEEAGEEEFKWWYNIFGEDYYVELQRHGIPDQDKVNDVLKKLADKYNVKVIATND
                     SHYVDVSDYNAHDILLCINTGEKKSTPIVKEYLEDEEGAKKNSRFGFYNDQFYFKTTQ
                     EMSQLFADLPQAIDNTNDIVDKVEYLDLKRDILLPNFPIPPNFLTQMQYLRHLTFEGA
                     KRKYGTITKEVEERLNFELQTIENMGFAGYFLIVMDFIKAGREMGVFIGPGRGSAAGS
                     AVAYCIDITNIDPIKYDLLFERFLNPDRKSMPDIDTDMDDEGRQKVIDYVVKKYGQNQ
                     VAQIVTYGTMAAKMSIKDVARVLDLPLNESLELTKLVPDTPGIELNRVFEAPLTGEKS
                     LSEKEGLNADDLNNVKRLREIISGNDLQAQVLKEARLLEGSVRNTGVHACGIIIAPQD
                     LSDLLPVAVAKDSELLVTQYEGKVIEEAGVIKMDFLGLKTLTIIKGALEIIKQNHGVD
                     IDIDAIPLDDAKTFELYQKGETNGTFQFESPGMQKYLRELKPDKFGDLIAMNALYRPG
                     PIEYIPNFIARKHGREPVSYDLPEMEEYLKDTYGITVYQEQVMLLSQKLAGFTKGDAD
                     VLRKAMGKKDRKTLDKLKPQFIANATAKGHDPEKLEKIWTDWEAFASYAFNKSHSTCY
                     AFVAFQTAYLKAHYPAEYMASVLNNASSIDKITFFMDECRRIGIQVLGPDINESKHIF
                     TVNKKGDIRFGLGSIKGLGENAVTAIVEEREKNGAFKDVYDFFERMNLSSVNRKAVEN
                     IILAGAFDLFGIQRSAFFAPDQQGVFLDTLMRYGNLFQADKAQNTATLFGDAGIANHI
                     STTKPQIPSVEPWTLLEQLKREKELVGMYLSAHPLDNYRFEFENICTHPLSELSDLSK
                     LKGKEIIVMGLVTDIRDGITKTNKNYASVTIEDFSGSFRFQLFGKDFENYYKVCIKNI
                     GLLIKGTVQTRKYNENELEFNISKIIHLSSVRDEFVKSLHLRIPSSYIDETFVSELTQ
                     KLETKDGKTLLKVSLIDPEENMMVDLISRMGKINISDNLIKFIHLNNGIKFSVLHN"
     gene            25501..26730
                     /locus_tag="PK252_02920"
     CDS             25501..26730
                     /locus_tag="PK252_02920"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOT13692.1"
                     /translation="MAFLNENYLKLKAGYLFPEIGRRVREFQTQHPQADIIRMGIGDV
                     TRPLTPAVIKAFHDAIDEMASVQTFKGYGPEQGYDFLRQAIVDNEYISRGADIEIDEV
                     FVSDGSKCDTGNILDILGRDNIVAITDPVYPVYVDTNVMDGRTGEVQDNGYYKGIVYL
                     PCTEANHFVPEIPKTKVDLIYLCFPNNPTGMTIGKDELKRWVDYALANDAIILYDAAY
                     EAYISDKAIPHTIFEIEGARNCAIEFRSFSKTAGFTGTRCAYTIVPKTLMAKDGKGQK
                     YPLNQLWLRRQTTKFNGVSYPVQKAAAAIYTGQGKKETAETIKYYMENAAIMRKSLSD
                     EGYKVFGGVNAPYVWVKTKNNMSSWDFFDLLLGKANVVGTPGSGFGPSGEGYFRFSAF
                     ADRQKVLEAMERIKKLG"
     gene            26748..27863
                     /locus_tag="PK252_02925"
     CDS             26748..27863
                     /locus_tag="PK252_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT13693.1"
                     /translation="MRIGFDAKRAFFNKSGLGTYSRETISILSDFFPTNEYILYSPRP
                     SKGKIFEKTDNLTVSYPQTFYYRTFSHLWRAYGLSGQLVKEKIDLYHGLSNELPMNIH
                     QYPVKTVVTIHDLIFLRYPKLYGYYDRKNYEKKFRYAAQSANRVIAVSRQTANDLFEF
                     FSIDEKKVDVVYQGCNPQFWQIVSEADKQGTASKYLIPEKFILSVGTIEKRKNILAVI
                     KAMHQHKIFYPLVVVGRPTDYLDEIRNYIEEHNLNNIYFLHDVPIDDLPAFYQMAELF
                     IYPSLFEGFGIPIVEALCSKVPVITSKGGCFAEAGGKSSIYIDPYNIDEIGEAIKSVL
                     NNNALRISMISEGYNHVQQFRLENIAGNIMRTYEKIL"
     gene            27880..28491
                     /locus_tag="PK252_02930"
     CDS             27880..28491
                     /locus_tag="PK252_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT13694.1"
                     /translation="MLDKITNGLFTFLYPSAVKKIKCSELSLFLTFDDGPTPEVTSQV
                     LKILAAYNAKATFFCLGKQVEAHPGLFKSIVEQGHAVGIHGYLHISGISITKSAFAEN
                     IEQCNRLISSDLVRPPYGRMTLSQYLWLIKRYKLVLWDVMAYDFKKEISVDTVVANVC
                     NNVRNGSIIVLHDTERTKDILVSALPIMLDKLKGYSFKKIDTF"
     gene            29676..30071
                     /gene="rsfS"
                     /locus_tag="PK252_02935"
     CDS             29676..30071
                     /gene="rsfS"
                     /locus_tag="PK252_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT13695.1"
                     /translation="MNRKKAEKNITDQELANAIIDAIQEIKGKEIVKIDLQKTGNAIC
                     DFFIICQADSTTHVNAIAENIEKTLWEQNKVYPHHVEGKENGLWVLLDFYNVIVHVFQ
                     QEYRNFYKLEDLWADGKIEKIKEPVKVTA"
     gene            30068..32134
                     /gene="ftsH"
                     /locus_tag="PK252_02940"
     CDS             30068..32134
                     /gene="ftsH"
                     /locus_tag="PK252_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT13696.1"
                     /translation="MNNAKNKDKQQEKPKKEKKQGLGGENLPKPKINPFGVYGLIAAA
                     FIGIQFFISSSGPQEIFMKDFKTMITAKDVDKIEIINKETARITLKKDRLDKYQKIVG
                     RNFSKIPKEGPHFEMQIGDQASFENSLKEFQADFPDNEKIYASSKTEKNYLFEILISF
                     VLPIVILIALWGFLYKRMTGGTGAGPGGANIFNVGKSKAKIFDKEASIQINFNDVAGL
                     EEAKVELKEIVDFLKNPKKYTELGGKIPRGALLVGPPGTGKTLLAKAIAGEANVPFFS
                     MSGSDFVEMFVGVGASRVRDLFAQAKAKAPCIVFIDEIDAVGRARGRSGGFAANDERE
                     NTLNQLLTEMDGFAPNTGVIILAATNRADVLDRALLRAGRFDRQIHVELPDLKERLEI
                     FKVHMKPLKLADDIDIDFFAKQTPGFSGADISNVCNEAALVAARKDKKAVEKQDFLDA
                     IDRIIGGLEKRNKIVSKLEKKTIAFHEAGHATVSWLLEHANPLVKVTIIPRGRALGAA
                     WYLPEERQITTREQLLDEMCSALGGRASEDIVFGKVSTGALNDLERVTKQSYAMVSYF
                     GMSPKIGNLSFYDSSGQSEFTFGKPYSEKTAELIDIEAKNIVEEQYQRAKQILTENRE
                     GLNQLAQILLDKEVLFSDDLENIFGPRKGGIPEEHQLLIKNGKTENETKEGNKPEPTE
                     NKDQQA"
     gene            32134..32340
                     /locus_tag="PK252_02945"
     CDS             32134..32340
                     /locus_tag="PK252_02945"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOT13697.1"
                     /translation="MEQGWVKIYKTDKDYQADIVIELLDENTIKAVKINKRDSSYQAF
                     GNIEVYVKEENTEKALQIIKKSAL"
     gene            32337..33149
                     /locus_tag="PK252_02950"
     CDS             32337..33149
                     /locus_tag="PK252_02950"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT13698.1"
                     /translation="MSEIGKRTISGGILVAVMLLCILGGSISFLAFFLLLIIGALWEF
                     YGMFFVVNIKPQRWSGIVITGILFLLSFFISAGLISSEWYLLVIPSFLYIVISELFRV
                     NDKPFENIAATVLGIIYIGLPVCLLWPIAYRDNIYQFNSHLVLGYFILIWTYDTFAYL
                     IGKQFGKRRLLERVSPKKSWEGAIGGLLVSFVTAVVIAHFFTDLSLFQWLVFALITTI
                     FGTLGDLSESMLKRSIGVKDSGNILPGHGGVMDRFDALFLAIPALYIYLQLI"
     gene            33162..33857
                     /locus_tag="PK252_02955"
     CDS             33162..33857
                     /locus_tag="PK252_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOT13699.1"
                     /translation="MTIKELIVNTTKLAQKKFRLHPEGKSIIANMLSWLLIANGILFF
                     LSISNTILTIIYILSIAAFLSLIYYFRNPERKVIPDEKYIYAPVDGRITSVTEVVEPE
                     YFKKKFVRVEISLSLTDIHVLRVPLSGKVKYCHFQLEDYRIAWHQLLSHKESASVGLI
                     SDTGTEIFLKQTGGAIVSYANENNYINQGNDLGFIIWGSSVDLFIPLSADIRVKAGER
                     VVGNITILAKATS"
     gene            34176..34823
                     /locus_tag="PK252_02960"
     CDS             34176..34823
                     /locus_tag="PK252_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOT13700.1"
                     /translation="MKKDYNTSRKKLALPEYGRHIHLLVDHIKNIADRDERNRAAQEL
                     INIMGNLNPQLRDVADFKHKLWDHLYVIANFELDIDSPYPVPTRESLNTKPRKVSYPQ
                     TSIKYKHYGKITEQIAKSVANFEEGAMKDALIEQLANIMKKQYLTWNRESVNDESIFK
                     DLSELTKGKINIHPDSLKLSETKAILSQKNKMHNKNNQQNNKNRNNHNKNKNKKN"
     gene            34848..36152
                     /gene="murA"
                     /locus_tag="PK252_02965"
     CDS             34848..36152
                     /gene="murA"
                     /locus_tag="PK252_02965"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT13701.1"
                     /translation="MAIFEVHGGKQLKGVLKPQGAKNEALQVICACLLTEQKVYINNI
                     PDIRDVNILIDILKSLGVEVVKTKEYEYSFDASKVYIDYLKTEDFRKKAASLRGSIMI
                     IGPLLARFGIGYMPKPGGDMIGRRRLDTHFIGFEQLGANFDFDAENNIYSVKADKLKG
                     TYMLLDEASVTGTANIIMASVLASGTTTIYNAACEPYIQQLCKMLNSMGAKITGIGSN
                     LLNIEGVNNLHGCNHNILPDMIEIGSFIGLAAMTQSEITIKDVSYENLGIIPNAFSRL
                     GITLERKNDDIFIPAQKEYTIDTFIDGSILTIADAPWPGLTPDLLSVFLVVATKAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGMGNEHKLRPTTMSSPDIRAGV
                     ALLIAALSANGKSTIHNIEQIDRGYYKIDERLNAIGAEIIRK"
     gene            complement(36334..36780)
                     /gene="rplI"
                     /locus_tag="PK252_02970"
     CDS             complement(36334..36780)
                     /gene="rplI"
                     /locus_tag="PK252_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT13702.1"
                     /translation="MEIILTQEVINVGSKDDIVNVKNGYARNYLIPNGMAIVATESAK
                     KILAENIRQRAHKEAKVKEEATQLAKKMEGLKLTIGAKTSSKGKIFGSVNTIQIAEAL
                     AAQGFNVDRKNILIKEDIIKEVGNYTAKVKLHKEVSVEIAFEIVSE"
     gene            complement(36800..37069)
                     /gene="rpsR"
                     /locus_tag="PK252_02975"
     CDS             complement(36800..37069)
                     /gene="rpsR"
                     /locus_tag="PK252_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT13703.1"
                     /translation="MSTNQSEIRYLTPPTVEIKKKKYCRFKKNKIKYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKYQRKVATAIKRARHLALLPYVTDMLK"
     gene            complement(37073..37423)
                     /gene="rpsF"
                     /locus_tag="PK252_02980"
     CDS             complement(37073..37423)
                     /gene="rpsF"
                     /locus_tag="PK252_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT13704.1"
                     /translation="MLKQYETVFIVTPVLSEPQMKEAVNKFKSFLTEHGAEIVHSEDW
                     GLKKLAYPIQKKSTGFYNLIEFKAEPALIAKLETEFRRDERVIRFLTFAMDKYAVEYS
                     NKKRSMKEPIKVEE"
     gene            complement(37520..39640)
                     /locus_tag="PK252_02985"
     CDS             complement(37520..39640)
                     /locus_tag="PK252_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT13705.1"
                     /translation="MLRLKRIKFNIDWEDFRVFWVIFSVSVLLLVILFPHKGKFKYEY
                     QKGKPWMHQVLVAPFDVPVYKTDEELSYEKDSISKHFIPYFTFNPKIGQEKREQFWQA
                     FTKKLQYINEGWARDTVVRKQLEELLKELLDTVYSKGIVNIPDEGASILHQSSTIAII
                     RDNEAEDVDANTLFTPKSAYEHIFQELVKFSDQVAFTDGNSPFNFLKSLKIEKYIIPN
                     LFYDAVTSEKVKEQALSNISLTQGMILSGERIIFTGDIVTPRHHKVLESVKKEYEQRM
                     GFSANYIFILLGQTIIVLTFIVLINVFISKLKRQVWRSNKNLAFIYTIIIVFGLITSL
                     IIKHRPGGLYMIPLAIIPVLLKTFFDSKLALYIHCLVIILFGFWAPNSFEFVMLNILA
                     GAVIVLSLSNTYKRNRMFFSAIWQTLTLSLTYVGLTLYQEGSLANINTDNLLYFAGNG
                     LLMLSALPLIYIFEKIFGYVSDATLLELSDTNQILLRKMAEVTPGTFQHSLQVANLSE
                     EAIFKIGGNVLLVRAGALYHDIGKIHNPAYFIENLSDEVNPHESLAFEESAKIIISHV
                     TKGVELAEKFHLPAQITDFIKTHHGTSLAHYFYRSYLNQNPGTEVDIKQFKYPGPKPF
                     SREMAVVMIADSVEAATRSLKHYSEAEINNMVDVIVNGLIADEQLVDADLTFRDIEII
                     KKTLKQRLRTIYHFRIEYPKGPAK"
     gene            39765..40553
                     /locus_tag="PK252_02990"
     CDS             39765..40553
                     /locus_tag="PK252_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOT13706.1"
                     /translation="MNIPTNAICTLPYIAVRKDPSETSEMISQLLFGEIYTVSDIQQK
                     WAKVVNQTDNYTGWCDAKLIKALSKEQVIELQNMKKVVVGQKWLDIHISCFDTSITIS
                     AGSILYQKQPGQFSINGENCNVSTNVIENYANTIDTVIYTAKSLLNTPYLWGGRSAYG
                     IDCSGLVQTCFRVAGKTLPRDASQQIYEGNTVSSLQDAQKGDLAFFCNEKGSVCHVGI
                     LLSNNQIIHASGFVKIDSIDIQGIFDKNKDQYTHHLCAIKRVTL"
     gene            40608..41510
                     /locus_tag="PK252_02995"
     CDS             40608..41510
                     /locus_tag="PK252_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOT13707.1"
                     /translation="MVQSLNNKFAPRGNIFFKSGKGGHVYAKIDNRFAACEVSLYGAQ
                     VLSFEPHGEPDIIWLSKKSHYDKGKAIRGGIPVCFPWFGPHAEEKTYPQHGFARLIDW
                     QVENVNNNPDGTTYIALYLCQNDFTKTYWPYDFEARVIITVGEQLKVELKVTNTGTEE
                     FSYTDALHTYFHVSNIEDICVEGLMNAKFYNGPGATQLEAQNVEQLTITKEENRRYIG
                     HSGACSFIDNGYNRKITIEKTGSNTTVLWNPWADTIKTMGDMPSDGYKNMLCIEPANA
                     FNDIIALKAGESHTLSTIIGYSKY"
     gene            complement(41867..42499)
                     /locus_tag="PK252_03000"
     CDS             complement(41867..42499)
                     /locus_tag="PK252_03000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOT13708.1"
                     /translation="MAKEWILNSAMNRFQLNFKRNVGPTSESIRKCSPKTLDEWREYY
                     FKNVKPKEHIVELGKKLYVKITEVIQSEVAEVSEQDCIDYMMQLVIDRTFDGYMTEIQ
                     TIYGQLQKILNIKIQPAPDEWDRLFNVDFFIQVKEKYIGLQIKPVNSGIQLPEIFKEY
                     NIQGDTHLKFTEVFGGKVFYLFSEKVGERKEIKNKEVIEDIKKEIERLNA"
     gene            complement(42492..43745)
                     /locus_tag="PK252_03005"
     CDS             complement(42492..43745)
                     /locus_tag="PK252_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT13709.1"
                     /translation="MKELPDNSVHLAITSPPYWQLKDYGTDNQIGFHDSYENYINNLN
                     LVWKECYRTLHNGCRLCINIGDQFARAVYYGRYKVIPIREEIIKFCENIGFDYMGAII
                     WQKVTTSNTTGGGVQMGSYPYPRNGILKLDYEFILIFKKLGDSPKPTKEQKELSKMTA
                     EEWNTFFAGHWNFAGARQNGHIAMFPEELPKRLIKMFSFVGETILDPFAGSGTTSLAA
                     KNTDRNSVGYEINPEFIPIIKEKLEIHQKDLNGTTYEFIQQKPITTDFEKEIQKLPYI
                     FKDPHTLDKKIDIKKLQFGSRIDKESSTKREEMFTVKEIISPEKIRLSNDLTIKLLGV
                     KEDPLVNGKATSFLIEKTKGKRVFLKYDIVKHDSENNLLCYLYLENKTFINAHLIKNG
                     LVQVDNDIDFKYKDKFLNLLHQNNG"
     gene            44017..46068
                     /locus_tag="PK252_03010"
     CDS             44017..46068
                     /locus_tag="PK252_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13710.1"
                     /translation="MKQKNKMNRAVAQFRQWSNKRYAVFNSLHRSIKICTLALAYSLV
                     SKPVTVKAESADTLQIKNQEIDDVVVVSSLLELKNFQTGRSVEIVQPTQIQSSPATSI
                     DDLLRYIPGVESQTRGAFGSQTDFSIRGSNFNQVLVLIDGHPISEPLTAHYNSNLPIT
                     PSEIDHIEVIKGPASGEYGADAMGGVIHFISKTFAKNQQAKNLQAEIKALGGQHALLQ
                     TNAGGYFQTDRWRFAAGGFINKSDGNKLSSGLYNFFDNRTYSFSTRYNLSDKWSLAYR
                     GAIDDRHFNAQYYYWANMQDSAEETLSRQRHHAQLCRASDAYTTTISGSYLNTNDIYK
                     TNSIYPKNDQTTEVAALKINQQWTVNDKIRLAAGTDVDLRRIESNNRGNHGTYKYAIY
                     LLASIRPTDKLSVNTSVRNEKHEKYGNHLLPQLSLAYKIIDNINGRLAAARAIRMPDF
                     TELYYNKLRQNVPVNSRMGNPELKVEKSWNTEVGVDFFLERLANISITGFYRETKGLI
                     DYVNILSSEVPFLTNLLPNTTYNVALNNSTTKTSGLETNISRKVSLNSKSYIQVLAGY
                     TWLHVDASFEKNALPYYLGNHAKHNLNGTATIRLSNLQIEVNGLYRIRDAKYEAAINR
                     YMAGGYALFNTSTSYAFYKERISAIVQINNILDRKYSDFPGVDAPGRWIAAGLRIKLE
                     N"
     gene            46645..47700
                     /gene="mltG"
                     /locus_tag="PK252_03015"
     CDS             46645..47700
                     /gene="mltG"
                     /locus_tag="PK252_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT13711.1"
                     /translation="MKLNNLLQSRTSKIILLIALAFIIFLAIQTGIFLKRIYSVNIEK
                     NEPGYLYIHTGATFTDVFNELERNNFLKDRNSFIWLAGKMNYVNRIKPGRYKITPGMS
                     NKDLIVKLRNGEQEPVDIPIHDVRTIPQLAGLLDRYLEIDSIHIVEKLSNEAYLDSIG
                     FNKNNVLAMFIPNTYELYWNVSLDKFVQRMLKEYNRFWTTARKEKAKEIGLSQDEVFT
                     LASIVEEETNGISEYPIIAGVYINRLKRNMPLQADPTVKFAIGNFAAKRVLKKDTGFD
                     SPYNTYRYKGLPPGPICMPSIKAIDGVLNYQKHKYLYFCAKEDFSGFHVFASTLVQHN
                     RNAEAFQKAMNKRGIYR"
     gene            complement(47771..49420)
                     /locus_tag="PK252_03020"
     CDS             complement(47771..49420)
                     /locus_tag="PK252_03020"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOT13712.1"
                     /translation="MLIALAQQNYIIGNFEYNCKKIVEAIEKAEKQNVDLIVFSELSV
                     CGYPPHDLLEDEYFVKKSLECVKEIALHCGKIAAIVGAPSVNTNPQGKGLFNTAFFLH
                     DKKIIAQYHKALLPTYDIFDEYRYFEPGSEFKVIEFRDERIAITICEDLWDEIPDESI
                     PYKRARMYNVSPMAEMAAQRPSLVVNIAASPFSYSKIRLKQEIFCNHAKNYRLPLLYV
                     NQIGAHTDLIFDGGSMVIDSKGHVAGALNLFEEDFRIIDSKNITLEDKRSIEKPEVEK
                     MHDALVLGIRDYFQKSGLKKATLGLSGGIDSAVVLVLAVKALGAENVRVLLMPSKYSS
                     GHSVGDALKLAENLGVQKDIINIQSVVDSFKTSLSPIFANLPEDITEENIQARTRGVL
                     LMAISNKFGHILLNTSNKSEIAVGYGTLYGDMNGGLSVIGDVYKSDVYKLAHFMNKDK
                     DVIPQNIIDKAPSAELRPDQKDSDSLPDYVLLDAILFQYIENRKCKKEIIDMGYDKAV
                     VEKTIKMVNFNEYKRFQTPPILRISSKSFGFGRRMPIVAKY"
     gene            complement(49432..50106)
                     /locus_tag="PK252_03025"
     CDS             complement(49432..50106)
                     /locus_tag="PK252_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOT13713.1"
                     /translation="MAKGAVIFFILLGVVQVFQAQESKYKFTVFANPCINWYASDVKS
                     TKTGGATFGYDVGLNMDKYFAENYAFSTGISIASLNGSASNTYAYSFSTKDDTSAVNL
                     PANTNVKYNLQYLVVPFGLKFKSHEIGYTTIYAHLGLTSFMNINAHATSGDAKGLLDN
                     SNITDEIHFFNLGYHIGAGVMYSLRGGNAIVAGLTYTSGFVDITDNSKDKVTTRSIAI
                     RLGFMF"
     gene            complement(50271..51320)
                     /locus_tag="PK252_03030"
     CDS             complement(50271..51320)
                     /locus_tag="PK252_03030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT13714.1"
                     /translation="MIGQDSLLRHVKLLFLGDIMGHMPQVKAAYDSSNDTYDYAPCFQ
                     FIKEPVLKCDLSIGNLEVVFGGKPYTGYPTFSSPDDLVFELRDFGIDVLATANNHCYD
                     KGKSGMYRTLKMIDSVQLLRMGTYYNAEDRASKCPLIVEKNGIKIALFNYTYGTNGFV
                     PDPPSVVNYLDRDQIKADVKVAEEKRAEVKIAFVHWGNEYELTPSDYQKKYARMLAQM
                     GFDAVVGSHPHVVQTFEWVTVDSVNKMPVFYSTGNFISNQRDRYKDGGAVVTLNIEIT
                     SGKVDVKPMYIPYWVYKGTLSSRYQYYVIPIQYYQLHSDSLHLPPDAAARLKEHAEDL
                     RIQLPNILIDTTYFH"
     gene            51541..51969
                     /locus_tag="PK252_03035"
     CDS             51541..51969
                     /locus_tag="PK252_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT13715.1"
                     /translation="MIQTTPNKDLEQLLKPNLFESVNILIAEDDETNYFLIKEYLEFS
                     NANLIWAQDGKEAIDILNQNNNIHLVLMDIQMPVMNGYDAFNLIKKSHPKIPVIALSA
                     YAVSGDREKGLRLGFSEYLSKPVSRKKLVEIILQKIEGSC"
     gene            complement(51966..53339)
                     /gene="rlmD"
                     /locus_tag="PK252_03040"
     CDS             complement(51966..53339)
                     /gene="rlmD"
                     /locus_tag="PK252_03040"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT13716.1"
                     /translation="MQKFHDVENVELVGLHSSGKSIGKWKNRNVFLEGGIPGEFVNLN
                     IAKRQKKAFLEGSISKILNASCYRQKPACKHFVQCGGCNWSHIQYLHQLVLKRQILIN
                     ALDKYEIKAGNLHKTIASPNIFAYRNKLDFAFANEIGVNGDNNIMFGFHPSGNPQQVF
                     KVEECLLMDSKSIEVCKFIVDKASEINCSVYDYQSKQGLLRGVTLRKNLDDKWMLILQ
                     VSENNTEMINTLLNQIMERFSFVVSVYCCVIRGNDARYEDYQYIYVAGEKYIFETLDE
                     IRFRISPQSFFQPNPLQAVNIFNKIAQIAEVSLGKFVYDLYTGVGTIPSFLSKHGCSV
                     LGIEGNKYAIEDAKYSAEANGFNRLKFMVGDILDTFTRDFVDKHGKADVVILDPPRSG
                     TLMEIKKAIVYAEPETVIYLSCNPVSLAWDLKFLGEFYDVKELWPYDMFPNTHHVETL
                     ALLKKKR"
     assembly_gap    53398..53497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            53723..54607
                     /locus_tag="PK252_03045"
     CDS             53723..54607
                     /locus_tag="PK252_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT13717.1"
                     /translation="MLSICIPTYNFSVYTLVQSLSQQAVKEHINTEIIVIDDCSSDEF
                     KKNNQNINNLSLVKYIELPKNIGRSKIRNLFLQYARFDCLLFIDCDAKVFRDDFIRHY
                     IENIGKAEIIVGGVAYDKEKPKKSIRLRWEYGHKREYTPLGNRLMKPYDSFKTFNFLT
                     SRNLIENIGFDQSISKYGHEDTLFGIALKNAGVSILHIDNPLIHIGLEPNNIFLSKTQ
                     KAIDGLWELYSSRKFDKNSNIRLLNTYLNLNIAISKCLALSFPVFSTLLKITIWFGLP
                     SLVLFDLYKLSYLCKIAK"
     gene            54833..57862
                     /gene="secDF"
                     /locus_tag="PK252_03050"
     CDS             54833..57862
                     /gene="secDF"
                     /locus_tag="PK252_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOT13718.1"
                     /translation="MQNKGAIKLLTILLTLVCLYQLSFTLVTYIYEKKAKNYAQGDLN
                     KETMYLDSIAGKEVYNFLYLKKFTYRDCKEREINLGLDLKGGMNVVLEISVADIVNAL
                     SNYSQDSTYLKAMSIARENLKTSQKDFITLFGEAFKQVDPNARMAAIFGTRELKDKIN
                     YNTSNEEVLKVLREKSNGAIDNSFKILRTRIDKFGVVSPNIQKMETAGRLLVELPGVK
                     DPKRVRKLLQGAANLEFWETYENSEIYNSLLEANKRIKALEESGAVDTTKTATNATNN
                     SAKANKNVAKKDSTKAEVALLEKLQKDTTKTDSISTPSQFNKEYPLFALLRPAITRDN
                     QLMPGSVVGYASHIDTAKINFYLNQKQVRSAFPRDVKFLWAVKAVKYDPSGSTFELHA
                     IKVTSRDGRAPLTGDVVVSARSDFNQKQATASEVSMSMNGEGTKIWARLTRDNIGRCI
                     AIVLDNAVYSAPRVNSEINGGNSQITGDFTKEEADDLANVLESGKLPAPAHIIQETIV
                     GPTLGKESIISGLLSFLVAFIAVLLYMWLYYSRAGAVANVALFANVVFLLGTITSLGT
                     VLTLPGIAGMVLTLAMAVDGNIIIYERIREEVRAGKGIRLAVKDGFWHAYSAIIDGHV
                     TTILTGIVLFIFGSGPIQGFAVTLIIGLLLSLFSSIFIARLMFEWMMDKEFHISIGNK
                     YTMEIFKHINIDFIGLRKKMYIISSILIGIGIVSLFIRGLDPSVEFTGGRTYIVRFEK
                     PVKTTEIRNMLTPTMQSAPEVKTFGEDNQVKITTKYLIDDKSAIADSLVEVALFKGLS
                     SKLPSGMDFTQFTEQQQGKAYGLMSAQKVDPVMSKDLVYQAFLAVIFGLIIIFIYIAI
                     RFKNWKYGLGGVIALFHDTMIIIGLFSLLYKFLPFSLEVDQQFIAALLTIIGYSIMDT
                     VIIFDRIREYNKLYPKRALAENINGAINTTLGRTINTSGTTIVTLLVIFIFGGEMLQG
                     FVFALLIGVIAGTYSSVSIATPIAYDLINMISKKKKA"
     gene            57963..58475
                     /locus_tag="PK252_03055"
     CDS             57963..58475
                     /locus_tag="PK252_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOT13719.1"
                     /translation="MDNFSKITDDSQNREYSNDEITVFWKPGKCVHATTCYKELIEVF
                     NPRRRPWVNMYGAPTERIIEIVNKCPTEALTYKWNDEAKNKETINLKNERLKKGEGQN
                     VTSQQAKPVTIQVMRDGPLVVQGDFEVFDEKGNKLKHMIMTSFCRCGASNSQPFCDGY
                     HRKIGFNSEK"
     gene            58482..58673
                     /locus_tag="PK252_03060"
     CDS             58482..58673
                     /locus_tag="PK252_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOT13720.1"
                     /translation="MEISNNNIPEFKVIANGPLHVKGKITLINTNGEKQELEETWLCR
                     CGNSKNKPFCDGSHKTAGF"
     gene            complement(58670..59869)
                     /gene="sucC"
                     /locus_tag="PK252_03065"
     CDS             complement(58670..59869)
                     /gene="sucC"
                     /locus_tag="PK252_03065"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOT13721.1"
                     /translation="MNLHEYQSKQLLKQYGVPVPEGFVAQSAGAAIEVAKKIADKTGT
                     NAWAVKAQVHAGGRGKSGGVKVAHNLEEVKHYAHDMLGMTLVTPQTGPKGKLVRKVLV
                     EQSVYYTGVSEIKEFYLSILLNRDKGMYAIVYSAKGGMDIETVAASNADVIYTELIDT
                     KIGLHDYQSRKIAYNIGLTGKLVNEFLLLLGNMFLAFEKLDASMIEVNPLVKTSDDKL
                     LVADAKVVIDDNALYRQPSLVGLRDIDEEDPAEAEAALHGLNFVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGNPANFLDVGGAASAKTVEAGFRIILKDKNVKAILVNIFGGIVRC
                     DRVAQGIADAFKNVGEIKIPIIVRLQGTNAKEGRDLLVSSGLKVMTATTLEEAAELVK
                     KAVTAQC"
     gene            60022..60558
                     /locus_tag="PK252_03070"
     CDS             60022..60558
                     /locus_tag="PK252_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT13722.1"
                     /translation="MRKLLNSELKRLTPDEFKKADKIPVIIVLDNIRSQHNVGAVFRT
                     SDAFLIEAICLCGITATPPNAEIHKAALGAEDTVDWKYYKTTSEAISELKEAGYSIIT
                     VEQAENSTPLEKFHAHSTKKYAIVFGNEVKGVDQEIVDISDKCIELAQYGTKHSINVS
                     VIAGIVIWELFKQLKLER"
     gene            60602..62962
                     /locus_tag="PK252_03075"
     CDS             60602..62962
                     /locus_tag="PK252_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13723.1"
                     /translation="MKKKILAFLLLSLCSQLIFGQRYTISGTITDASNGELLPYSTII
                     DSVSKKGTLANYYGFYSIKLDKGPVKIMCSYAGFNPSIISFNLTKDTTINLTINSTTE
                     IEEIVVTSSTNKLRSTQMGLVEIPIQETKQLPVLLGEADLMKTIQLMPGIKGGTEGTS
                     GIYVRGGGPDQNLILLDGVPVYNASHAFGVFSVFNSDAIQSFNLMKGGFPARYGGRLS
                     SVIDIKMKEGNNKEFHGEGSIGIISSKLTLEGPIKKEQSSFLISARRTYFDLFTVPIT
                     MAASDGKNVGGYYFYDVNAKVNHKFSEKSRLFLSFYTGLDKVYMREKSSYEGNTRKNE
                     AGTGWGNITTALRWNYILSDNLFANFTGTFSQYKFFIEEESNNSNNNDFFSYQYNSGI
                     VDYAAKIDFDYIPHAAHNIKFGTNSIIHTFNPGVNVQKENGGNNNIDTTFGNKNIYAT
                     EISSYIEDDIEFSPKFKANIGLHYSFFSVRSRTYNSFQPRLSGRYLLNNNIALKASYI
                     QMMQYIHLLTNSTIGLPTDLWVPSTPNIKPQEAWQTALGTEISLNKGYSLSVEGYYKE
                     MKNLIEYKEGASFFSEKDDWEGKVVQGKGWSYGAEFLLSKSTGKITGWIGYTLSWTNR
                     QFESISFNKVFPYKYDRRHDIAIALTYKHSETFDMGATWVYGTGTATTLATEEFNGYD
                     TFFDNFNIPKLSGAYVHYYENKNQYFEHRNNYRMPAYHRLDIGFNFHKQKKWYKRTWS
                     IGAYNAYNRQNPFMLVLEKDYESGQNKLYQYSIFQILPYFRWSIKF"
     gene            62965..63969
                     /locus_tag="PK252_03080"
     CDS             62965..63969
                     /locus_tag="PK252_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOT13724.1"
                     /translation="MRTKHLAFSILFVFIALIIGGCRKEIPLNIDSKDRKIVVNGILI
                     PDSAVCINLSKSSSIAEKDLNEYRYIENANVGLFENGILVTHLTSTNNGYYKSDNDFR
                     PKAGNFYKLIVEHEGKTASSEFFLPKKELNHSIDVKKIRSYSFEKDDSVVYSLKIYIS
                     INDNGNNKNYYCISFGYPVFQNRYYGKDLPISQIIIYNSKFTLYSPAVEFSNFNYLND
                     DEKLEKGIYGQFVYFSNELFRGQKHNLRLETNLSPFYVKEGKDSATFYLYISMIDENY
                     FRYRKYIAEYDNDDDFFSEKVQIVNNINNGIGLFSGITCIVDSFSIAINEFPSMLIQQ
                     "
     gene            64174..64599
                     /gene="ruvX"
                     /locus_tag="PK252_03085"
     CDS             64174..64599
                     /gene="ruvX"
                     /locus_tag="PK252_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT13725.1"
                     /translation="MGRILALDYGTKRTGVAVTDALQITVNSLGTVATKDVWSFLETY
                     FAKEVVDCVVIGYPLQMSGKGSDSLRVINPFIQGFIKKYPNIRIVQEDERFTSKIAQQ
                     AILAGGVKKKDRQDKALVDTVSAAIILQSYLDRLRLTNR"
     gene            64612..65166
                     /gene="def"
                     /locus_tag="PK252_03090"
     CDS             64612..65166
                     /gene="def"
                     /locus_tag="PK252_03090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT13726.1"
                     /translation="MILPIYVYGQPVLRKVAENVTKDYPNLEKIIADMWETMEKSDGV
                     GLAAPQIGKSIRLFVIDASHFAEDDPKTEGFRKTFINAQILSREGEEVLFNEGCLSVP
                     GIREDIKRPSIVTIRYMDENFVEHEETYDGIRARVVQHEYDHIEGKLMIDHLNPLKKT
                     LLKRKLNDISKGIVDVNYKIRAVK"
     gene            complement(65255..65845)
                     /locus_tag="PK252_03095"
     CDS             complement(65255..65845)
                     /locus_tag="PK252_03095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT13727.1"
                     /translation="MIKRLYNENPNERDVEAVADILRKGGVIIYPTDTVYGIGCDICN
                     KAAVERIARIKQTKTEKANFAFICYDFSHLSDYARQVDNATFKLMKRLLPGPYTFILN
                     ASSNVPRFFLEKKRTVGIRIPNNNIIREIVKALGNPVLTTSVHSDNDLYEEITNPELI
                     HEKYEKLVDLVIDGGIGGTVLSTIIDCTGDELVVTR"
     gene            65955..66389
                     /locus_tag="PK252_03100"
     CDS             65955..66389
                     /locus_tag="PK252_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOT13728.1"
                     /translation="MDLREILSISGFGGLFKLISQSRNGLVVEGLEDKKRMNAFSHYK
                     VSSLHDIAIYTDTEEIPLRKVLAKIYEKENGQQAFGGKIDEKQLKAYFETILPDYDRD
                     RVYVSDIKKVISWYNILVRCGFTSFEDAQEAKKDETEKATKE"
     gene            67356..68150
                     /locus_tag="PK252_03105"
     CDS             67356..68150
                     /locus_tag="PK252_03105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOT13729.1"
                     /translation="MTKKYSTSFSLATSLLEKEKRKAIYAIYGFVRLADEIVDSFHGF
                     DKPFLLNNLEEQLHYALQNGISANPILAAFARTVKKYHIDNDHIQAFMQSMKYDLTKT
                     DYTSNEELNSYIYGSANVVGLMCLKVFCNGDQTHYDKLEFSAQKLGAAFQKVNFLRDL
                     DKDKKELGRNYFPEIWDNTLKIDNKQRIEQSIEDDFNEAWKGIKQLPGRAKLAVAIAY
                     FYYRALFGKIKQTTPERLMAERVRVNNLNKYLILLKVGMMYKTRLF"
     gene            68192..68851
                     /locus_tag="PK252_03110"
     CDS             68192..68851
                     /locus_tag="PK252_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOT13730.1"
                     /translation="MNLYPRNILPQKEVKKFVVIFYTVGLLGFIIPFSRDFFIKITPL
                     ALLISSYLLFTYHNNISKKDVAAFMLVFVLGFLIEAAGVNTGQIFGHYSYGSTLGPAL
                     WGTPLLIGLNWLFLAYSSLVVAEKISHKAILQIAIAPTLMLIYDLVLEQVAPILDMWT
                     WQDASVPLKNYIAWWCIGLAFTGFLKISKTDTSNPLALILLVCQFAFFLLLFLVFNLL
                     L"
     gene            68848..69468
                     /locus_tag="PK252_03115"
     CDS             68848..69468
                     /locus_tag="PK252_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT13731.1"
                     /translation="MIKARHHIVVYPLFKWLTRFLIKRNFRAVHVEGYFTDNHKAILT
                     IANHVSWWDGFWAEYLNQKIAQRRFYFMMLEQQLKKHWYFSYTGGYSIKTKSREVVKS
                     IDYTAELLHNQENIVLMFPQGKIHSAYNNDIHFEKGVQHIVNKSPDDMQVLFVANFTD
                     YFSDAKPHLYIYHEAYPALFLKKNDIEKEYNQFYTRVLNKHKIKAS"
     gene            69468..70568
                     /locus_tag="PK252_03120"
     CDS             69468..70568
                     /locus_tag="PK252_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT13732.1"
                     /translation="MLYLLYISSAFIALQLFNVLLNFLFRQKIENTDRQNSCMLSVLI
                     PARNEEANIGQLLEKLHNMKNEHVEIIVCDDHSEDKTIQIAREYCKKDPRIQLMLSDT
                     LPTGWLGKNHACFQLAQKARGNYFLFVDADVRLHGNILADAVSYMKKHRLGLLSVFPK
                     QIQQTIGEKASVPIMNYILLTLLPLIFVRVSPFKSHAAANGQFMLFDAETYKKFQPHE
                     QFKKSAVEDIEIARFFKHHKIKTACLTGETRIECRMYTSYQEALNGFSKNIFMFFGNI
                     PVLAFIFWTCAALGFLPFVMPGQQLLAEYLIAIVLIQVLYAASCKQPVFYTLLLFPVH
                     LAFMLEMMVKALIVKYNKKYVWKGRNIYKLHS"
     gene            70532..>71251
                     /locus_tag="PK252_03125"
     CDS             70532..>71251
                     /locus_tag="PK252_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13733.1"
                     /translation="MERKKYIQIAFISLLLYPLSGQNNHTIYHAFAAGNMKKWKTAMD
                     SIEAITHKSNKELLDLLNYQYGYIAFNLGKKNAQEADKYMIKAYTDLKLLEKNKYNLP
                     VLYAYKAALTGFEIGLHPYKAPFIGPKSIEYAKISVKLDSANAMGYIQLGNIAYYMPS
                     MFGGSKAEALIYYLKALSISEKKIECKNHNWNYLNLLSIIIKAHMDLGQYHHAETYCK
                     KALAVEPGFDWVKNNLYPQVLK"
BASE COUNT        22677 a        13563 c        13357 g        21554 t
ORIGIN      
        1 agatatctta ccagaaatgg cattccggca ttgatgtctt tgttgttttt tagccattct
       61 tcgatgtata gcctgaaaac ctgggcattg gtaattttat catatatccc ggggctgctt
      121 ttttctatgg cacgttctac caaagggttt ttcttaaatt cttcaagttg ttggtcgtta
      181 caaaaaaaga tactttgcat gtcaatgttt atagatcttt ttatacgtct tcctaatgag
      241 atatcaagcc cacgccagtt taaaaatgat tcgttgacga aagaataggt aggaatagta
      301 acaatagtcc ggttagcatt gtgaacctta acagtgctga gttttatatc aataacagtg
      361 ccatcagctc cgcggcttgg catttctata taatcaccta cacgtaatat gtcgttggag
      421 gagatttgaa tacctgcaac caaacccagg atagcatctt taaaaacaaa catcaaaaca
      481 gctgcaaagg ctgtcaatcc tgcaaacaac gaactcaggt ctttttttac caaaatggag
      541 attaccatca acacaccgat aaaaatcact atagatttgg cagcctgaat gtaacccttt
      601 attgaacgtt gtttgcccat gggggtcatg ttgtatatat catgcaaggc attgataaca
      661 gccattatta cagctataac agctaaaagt atgtatatgt tggttaaagt agcaatggta
      721 ttgatagcta cgttgtaccc atataatgca attggcgtaa gttttgatat caccaatgcc
      781 ggagccaggt gagataagcg gttgaatacg cctttttcat aaaatacatc gtcatagtca
      841 ttggtgcttt tatctaccca ctttttgatg atagaaatga tgattttttt ggccacaaag
      901 ttggccaaca ttgccattaa agctatggct actatgttgg ccataagatt aagagaatgt
      961 gctactgttg gtgatagatg atgcgataat atcaactttt caagagaaac acttagttct
     1021 ttcagatagt catacatagc ttcttatttt ttttgcaaaa gtaatcattg cataaaaaaa
     1081 gcacacatct tgttaaatgt atgctttctt tttttgttaa aaaagcttat tgtttttttg
     1141 cttcttcttt tttctgttcg ggggttgaaa tagataaaag ctcaacttca aaaattaata
     1201 atgagttggg tttgattttc ccttgaggat tagggccata acctaattgc gatggaatgt
     1261 agattttgta gtttgacccc actttcatca gttgaagggc ttctgaaaac cccgggataa
     1321 caccatttac ggcaaatgtt ataggctctt tgcgttcgta tgagctgtca aatacagtgc
     1381 catcaattag tgtgcccttg taattgatgg taaccatgtc ctcagctttg ggggttgccc
     1441 ctgtgccttc tcttaatact tcatattgca agccgctggc ggtagtgata acacctttgc
     1501 gttctttgtt tttcttcaaa aattcttcat tttttttcaa tgaagcttca gcttgttttt
     1561 tttgaaggtt tgtgaaatat tgttgtaaga acatttgaat ttgctcttgg gtcatcttgt
     1621 gttctttttt ggccattgct tccataacgg cctgtttgaa aagttcagcg ttaaaagccg
     1681 gcatgtcaag cggttgtaaa tttgcaccta ttgaaacccc aatggcataa ctggcactgt
     1741 ccacatcgtt acggagtttt acagtgcttt ttttattaca tgcacttaat actaccaacg
     1801 aaattgctaa aatcgaaact aaaaatttta ctttcattgt tttatattta aaaaaattaa
     1861 taattattta ttttctgcag gagtttgttt ttcaaccgaa ataagctcaa tgtcgaatat
     1921 caatacactg tttggctcaa tctcgctgcc gcgcataatc tgctcgccat aagccagttc
     1981 tgatggaatg tacaaaatcc attttgcccc gggtttcatc agttgcaggg cttcggtcca
     2041 tccgctgatt acgccattta cggcaaactg cataggcttg cccctttgta ttgagctgtc
     2101 aaatactttt ccgttgatta atttcccttc ataatggcag gtaaccttat cattgatggt
     2161 aggcgactct ccttcgccat ttgcaatcac tttgtattgc aaaccacttg gaagggttat
     2221 tacgccttct ttctttttgt tttcttcaag aaacttgcgg ccttctgcca ggtttttttg
     2281 cgttttggcc tgaaattgct tcatcataaa agcttgaatg agttgattag caacttcttg
     2341 cgatatttta gaggtgccac taagttcagc ttccaaacct tttataaaag cttcgttatt
     2401 tattgcactt acaccagctg cttttagcga attgccaaaa taagttccca aacaatacga
     2461 tacagagtca aggttggttt ttaattcttc tttctgtttc ttttgcgaaa aagcattgaa
     2521 ggcaatgctc ccaataaata taaaggtcag aatttttttc atgtgttttt tttttactat
     2581 aaaacaggtt atgttcttta caaaacggca caaaggtata ttttatttgt tcattttgat
     2641 atggttagga caataattta ccaaccgggg aataaattga ggttcaggcc ggcatagctt
     2701 tgccagtctt tcataaatgg gatggaataa tatggttcga tgattaacac cccgaaaagg
     2761 ttaatgcgca aagaaacccc ataggtcata agcgggtatc ggtaatagtt ttcgtctaag
     2821 ccatcaaaca tattatccgg gccagatgta gccaggtttt tatagttgac ttttaacgtt
     2881 tcgtttttgt ggcggttaag ggtaagttgg tctattttac tccaggctac accagcatct
     2941 gtaaagaatg aaagctcgga aacaaagtat ttaaaaggta tcaggcataa ccgttcgggc
     3001 ccggtaaacg ggatcctgaa ttcagcattg gctatggcca aacggcttcc aaacagcatg
     3061 gtttgattgg ccagataact gtctgccccg ataaatccgt aaaacccacg taccaaccac
     3121 ggccatccta agtacaacga ccttattaca ttgtttgtag tcacattgcc atatcttccc
     3181 tggtgataaa gtctgaaggc aaagctgtaa gggttgataa agaagtattt ccggtaatct
     3241 atcaataaat tggtaaagtt tatcttgcca acatattggt ccacttcgac cctgaagcgc
     3301 tgtccacgta atggcgaggc tatgcccatg tacgaattgt caagtgtata ggctgtactc
     3361 acagaggcaa taccaaatcc cttgtctgtt ggcaggttct tttcgcgtcc aatccaggtt
     3421 gaatatccat aaatgcttcc tatatattta tcgcgttcaa gataaaaatt gtaatatgaa
     3481 tatgatatag aagcttctat tcttcgggtt tgcgataaag gttttatagc aaacaaagct
     3541 attgagtttt caaaaatcca ttgcatatct acatttagca ccgaaaccag gttgtcatcc
     3601 acactgtcat ttgaatagta ataattgcca tatttgtatg ggatgtgcga aagtgaagca
     3661 ccccagtcta tcttgttttt gttgttgata aaggctactg agcctccaaa gtcctgaatc
     3721 tgcccgttta tggccaggct ggtataaagc tggtaattgc ctactacatc accaaagagc
     3781 atgtccacac tgcccgacat acctgtccca taatatgacc ccgccgaaac gcctacattg
     3841 gagcctccta tgtagtccaa ttgaaatttt gaacgatagg ggaccgggat aatagagtct
     3901 ttgggcaggg tgcttttatc tatagcctcg cccaggttga cattgactat gtccttgcca
     3961 attcttttaa atggagggag tatagccgca tcaaaattca cttcgtctgt gtttactttt
     4021 accgctttaa aatcattgat attagctttg taaagattat aatctccgtt ccaataatag
     4081 gtatatatta tgttttggtg gttcatagtg agtgctggag taaaatgtgt caccccgcaa
     4141 actccggttt gggcattgct caaacgcttg gtttcattac ttgatacgtt gtaatggtac
     4201 aaatttctta tgccattagc gtctgacaga aaatacacat tgtcaccagt tgaatctatc
     4261 tgggcattga ggttttctgc accaggaaat accggaagta cttttatttc ttttgtttga
     4321 acgtcaaata tagctaatga gtaacctttc tggatgccag tttgcccttc ggccggccta
     4381 tcggtcgaat atacaataaa acggccgtct tttgaccacg aaggcatcaa attgcaataa
     4441 acgtcattgg tcaaacggaa tgttgtgtct ttttctacct ggtacgaata taaatctgta
     4501 acaccttctt ttaaacctgt aactacaata cttttaccat cgggtgacca tgcagggtta
     4561 ctaaatgcgt ctattgtttt caagtctttt tcagttgaat aggccggatt gtttatatca
     4621 ataatcaaca atcggttacg gcctttgcta aaagtaacaa atgcaaactt ctttccatcc
     4681 ggagaccatg taccgcccga ttccaggaag ttgaagtcgt ctatttggtt ttgtctgatg
     4741 gtgcttgaga ttttttttac aattttgccc gttttagcat cggccataaa aaggtcaaag
     4801 gtgaataggt ctttttccga aaggaatata acatatttac catccgggct cagcgacggg
     4861 gctatattga tggccccgga atttttgcgg tgcaggattt ttgtgccggt tggctgtttg
     4921 tcagctttgg ctatgaactt gctgtaatat tgctgcatgg agtttttcca ggcttgcgag
     4981 aatgaacgtt cgttccatat agttagtgtc tcaagggctt tgtcatatcc atactttgta
     5041 gtcaggtcaa aaagcggttt tataatggtg tctccatagt aaccagtaac aaaagcccaa
     5101 aaagcttgtc catacctgta cggaaagtat ttatatgagc ggctcatgtc atccagcgtc
     5161 ggaaaatcgt tgttcagaat ggcatcacgc atccacattg cagtaagcac gtcggtactc
     5221 ccaatagaca agtattctgc cataccttct atcatccata acggtatatt tcgcatattc
     5281 cgtagttgaa ggctgtcatc gctaagcaaa aagttgtatt gaaaagcatg caccagctca
     5341 tgccctaaaa cgtggtcagt ttgggcagcc gattccatca aaggcattat taccctgttt
     5401 ttgagcgatt cggttacccc tcctgtgcca acgcctatca acccgcttac tgcattggtt
     5461 tgctggaagt cggcatggtt ggcatacaat attaccgggt tcttttcttt aaaggtgtct
     5521 gaaaatatca taaaatggcg ttcgtaccat ttttcagaag ctgccataaa ggcatttttc
     5581 accgaatcgt tctctaaata atgataaagt tcaaaatgag gactagtaag gactttgtac
     5641 tcaaaaatct tgtaatcggg cttgttgggg ccattaaaat actgcccata cccatgttgg
     5701 gcgagtaaca gatatgaccc gatgaaaact atgaacccct tatatatatt gcttttaaag
     5761 ctatatttat ttaacacaaa ctctcttttt ttttctataa aaatgtaact aattaaccaa
     5821 tttcactaag ttttagtatc aattcttcat tattaattac aattttcttg ccatctaacc
     5881 taatcaaccc atcttcttta aactcagaca atagcctgat aacgctttca agtgccatcc
     5941 ctgaaagctc agcgatatct tggcgtgtaa tgtgcgaaaa tacatcagta ttgttaaact
     6001 cgctgctatt gagataaagc agaacgtctg ccaatctgcc atgcatctgt ttagtgctca
     6061 aagatgatat ttttcttata atatatgcat atttctccga aatagcatca aaaattatct
     6121 gggcaaaaca tgggttgctg gttatcaatt taaatacaac ttctttagga atgatacaaa
     6181 cttcgctgtt agttaaggct gcggcagaat attggcaaag atctttgcta aatgaattga
     6241 tgccaataaa agaccccggg gtcagtattt tcaatatagc gtttttttcg tttcggcgtt
     6301 ctatagataa ttttactaat ccgcttttta gataaaatat ttgtgttgaa cgtgtatttt
     6361 gtttgaagat tagctcttta cgtttgtaaa aaatctctga tacttcgttt aacaaaagac
     6421 cttgaccagt gtcttttata caaagataaa aaggacaacc actatagcac tgatggcata
     6481 atgttgaatt catgtttctt gtataattaa aaatgataaa agtttcagtt tttgccctgg
     6541 atgctaaaac ttactataaa tatacacaaa ctttcctatt ttcaagtaaa atatgagttt
     6601 taaaaaatga caaaatgtaa aaaaagattc ttaacttcac attagtacga gtagtttata
     6661 aaagaaattt tatgataaac actcttaaaa atattagctt tcatatacaa ggttttttgc
     6721 ttttttttga tattgattgt accctggtgt tttgcaatga tgatagtttt gtgcccgggc
     6781 tgatagggaa aaaaattggc gagcacgaaa tttcgtcaga aaaaaagctt cttattaaca
     6841 aaaacagaaa ggtttgcacc gttccgcaaa ctgccactgt caatattgaa aatgtcaggt
     6901 atgaaacagc tctttttacc tgcgaaaacg gtgaaaacga aacatcaggt tacatattga
     6961 ttgttagggc tcaaaaagag aatagaaatg gggaagatca ttactttgat gatgtttcaa
     7021 atttgttttt gtctaatatt gctcacgaaa tccgtacgcc gcttaatggt attattggtt
     7081 ttgctgagtt gctctctaaa aaagatttcc cggtggggaa aatacgtgaa tatgcccaga
     7141 ttatacatag caatggcaat tatctcttca agctcattac tgacttgctt gatttgtcgc
     7201 gtatagaagc tggtaagctt acattgttca aaagccagtt tagcctaaac cgtttgcttt
     7261 acgatttgca gttgtttttt atgctcgaca tgaaaaatcg caataaaagc catattagct
     7321 ttaagctatc tcttgagttg cccgatggga gcgattttat cactgccgat gagctccgaa
     7381 tcaagcaggt actgattaac cttattggca acgctataaa atttaccgaa caaggcacta
     7441 ttactgttgg ctataagtta gccaccaaag atatgttgga gttttttgtt caggattcgg
     7501 gtattgggat gccctcatct atgacagaga aaattttcgg ccgcttcgtc caagccaatg
     7561 ataaaatagc ctttactcac ggggggacag gtttaggatt ggctatagct aaagaatttg
     7621 ttgagttgca tggaggtaaa atttgggtag aaagtaaaga aggctgtggt gctacttttt
     7681 attttaccct gcctttgttt tttccttctt gaccagaact gataaataac aatgttcatt
     7741 tttttataag gaatataatt ttccaaaatt aagtttagaa aatatctaat tggataatta
     7801 tttgtatttc aatattttaa ttcaattaag tacggcaaaa aatagctttc tattgggtta
     7861 aaatatttgt aacaggtttt tattatttct gtttcaatgg gtgctttatg cattatttaa
     7921 aatgaatata attaagcact cacggtttga atttttatac aatatatttc attttcactt
     7981 acctttgtgc cctgttaaaa ataaaatttt tacaattcgt tttatataaa aacaataata
     8041 atatggcaga caaaaagttt gttacatgtg atggaaacta tgcagcagca catgtggctt
     8101 atatgtttag tgaagtagct gcaatctatc ctatcactcc gtcatccaat atggctgaaa
     8161 acgttgacga atgggcagca gccggtcgta aaaacatttt cgggcaaacc gttcgcgttg
     8221 ttgaaatgca aagtgaggct ggtgcagccg gagctgttca cggttcgctt caggcaggtg
     8281 cattgaccag tacatttact gcgtctcagg gtttattgct tatgatccct aatatgtata
     8341 aaatatctgg agaactactc cctggtgttt ttcacgtttc ggcccgagct cttgcagcac
     8401 aagcgctctc tatatttgga gaccacagcg acgtaatgag cgcccgtcaa acagggtttg
     8461 ccatgttggc tacaggtagt gtccaggaag taatggacat agctcctgtt gcacacctta
     8521 cagctatcaa atctcgtgtt cctttcttac atttctttga tggtttccgt acctctcacg
     8581 aaattcagaa agtagaagca gctaaccaag aagaattggt taaactatta gaccaaaaag
     8641 ctttacaaga attccgcgaa agggctttaa acccagaaca tcctgtaaca cgcggaacag
     8701 cccaaaatcc tgacatttat ttccagaccc gtgaagctgc aaataaattt tatgcagcag
     8761 ttccagagat ggtcgaaagc tatttgggcg aaattaagaa aatcacaggc cgtgaatatc
     8821 acttgttcga ttattacggt gcagcagatg ccgaaaatgt tatcattgct atgggctctg
     8881 taaccgagac cataaaagaa gttatcgatt accttgctac taaaggcgaa aagataggtt
     8941 tgatttctgt tcacctttat cgtcctttct cagctaaaca tttccttgca acacttccca
     9001 aatcagtaaa acgcatttgc gttttggacc gtacaaaaga acctggagct aatggcgacc
     9061 cactttactt agatgtaaaa gaagtccttt acggaacagc taatgcccct atggttattg
     9121 gcggccgcta cgggttaggt tcaaaagatg ttactccggg tcagattatt gccattgtaa
     9181 agaacctgaa agcaaatgag cctaaaaacc aatttactgt tggtatcgtt gatgatgtaa
     9241 cattcaagtc tcttccggtt gagcctgaag taaatgttat caaagcgggt acttttgagg
     9301 ctaagtttta tggtttaggt tcagatggta ctgtaggcgc taacaaaaac tctataaaaa
     9361 ttataggaag tacaacagac aaatatagcc aggcatattt tgcttacgac tcaaaaaaat
     9421 caggaggtat taccacttct cacctgcgtt ttggagatac cccaatacgg gctccatatt
     9481 tggttgttag tcctgacttt gttgcttgcc acgttccttc atatttagat aaatatgaca
     9541 tgttggctgg cttgaaaaaa gggggtacat tccttctcaa tagtatctgg gatgctgaag
     9601 aaaccaaaaa acgtcttcca gaccacatga aaaaacaatt ggccgatgca gaagctaaat
     9661 tttatattat caatgcaact gccattgccg aagaaatagg ccttggtacc cgtaccaaca
     9721 ctatcatgca aagtgcattc ttcaaaattt caggagttat accttacgaa atcgctgttt
     9781 gcgaaatgaa gaaagccata gataagagct ttggtaaaaa gggagaagaa gtagttaaaa
     9841 tgaactatgc tgcagttgac cgtggtggcg ctgttattga agttcctgtt cctgctgaat
     9901 ggaaaaaact cgatgggact gttactattg gaaaaacttc agctaaggtc cctgacttta
     9961 tcaaaaatat tgttgtgccg atgaatgcca tgaagggaga cgaccttcct gttagtgcat
    10021 ttaataaccg tgaagacggt acttttgatg ccgggacatc agcttttgaa aaacgtggta
    10081 ttgcagttaa cgttcctgaa tggcaagctg aaaactgtat tcagtgcaac caatgctctt
    10141 atgtttgtcc tcatgcctgt atccgtcctt tcttgttcaa tgaaaaagaa gcagccggaa
    10201 tgcctgcagg tagcaaaatg attaaagcta atggaaaagc cctcgaaggt ttgcaatata
    10261 agatacaggt aagtccgctc gattgtacag gctgcggtaa ctgcgctgac gtatgtccgg
    10321 ccaagactaa ggcccttgta atgaaaccgc ttgaaacaca acaggcagaa attgcacgtt
    10381 gggagtatgt tgcaaagaac gttacttaca aagacaacct gatgcctaaa gatgccgctg
    10441 ttaagaatac tcagtttgcc caaccattat ttgagttttc gggcgcttgt gcaggttgcg
    10501 gcgaaactcc ttatatcaaa ttgattaccc aattgtttgg cgaccgcatg atggtagcaa
    10561 atgcaaccgg atgttcttct atttatggtg gttctgctcc tgctacacct tatactacaa
    10621 atagcaaagg ccatggtgtt gcatgggcaa actcattgtt cgaagataat gctgaatatg
    10681 gtttaggctt ggctactggt gtacgtcaaa tacgtgagcg tatagctttg aagatgaacg
    10741 agaatatgga agtggtaaat gcagctacca aagaagcttt tgctgaatgg attgcaggca
    10801 aagacaatgc tgaagcctca ataactgctt cagaaaaagt acgcgctgca ttgaagaatg
    10861 aaaaagctcc gatagctgcc gaaatagcca aacttgatca ataccttgta aaaaaatctg
    10921 tttgggcatt tggtggtgac ggttgggcct acgatatcgg atatggcgga ttagaccatg
    10981 tattagcttc tggcgaagat attaatatcc tggttcttga cactgaagta tattcaaata
    11041 ccggtggaca ggcctcaaaa tcaacccctg ttggcgctat tgctaagttt gctgcttcag
    11101 gcaaacgtat ccgcaaaaaa gaccttggga tgatggccgc aagctatggt tatgtatatg
    11161 tagcccaagt ttcgatgggt gcaagccagg ctcaggtact taaagctatt cgtgaggcag
    11221 aatcattccc cgggccatca ataattattg cttatgctcc atgtattaac catgggttac
    11281 gattaggtat gggcaaatct caaaaccagg ctaaattggc tgttgaatgt ggttactggc
    11341 aattataccg ctatgaccca cgtttggaag aacaaggaca gaacccattc cagctcgatt
    11401 caaaagagcc taaatgggat gaattccaga actttatcat gagcgaggtg cgttattctt
    11461 cactgaaaaa agcgttccct gctgaagctg aagaactttt caaagcagct gaagaaaatg
    11521 caaaatggcg ttacaaaacc tacttgcgac aatcaacgct tgattataca aaataatcaa
    11581 cataataaaa aggctacttt cgggtagcct ttttttcttt taaccttcca ggttgtgaaa
    11641 acctggaagg ttttttaata aacagcccta atggactttt tgaagcatcc tgataaattc
    11701 tgccatgtgc tgttgcgaaa aaacttgtat aactgcaaat actggcacaa aaacaagaag
    11761 ccctatcagt agccctttgc ctaaagaatc atactttgac tcttttagta gctcaaacaa
    11821 aacccaaatc gcattgagcg taatgatgat gtgggtaatt tgggcaaaca ggttggcggc
    11881 ttcccagcta acaacaaata agcccatagt gccgtagtaa tacattggta caaatgcaat
    11941 ggccaggaaa aagtcttttt tagtaatctt gtagcataca aaacacgtca gatatgctat
    12001 gatagcaagt gagcaggcat tgaatgcaag ccacctgaaa aagtcaggtt ttacaattga
    12061 taagaaaaaa taggcgctgt ttaacagggt taacagcatt aatgaggcta atacatagcc
    12121 tgcaatttgg tttttattat taatactcat tatttaaaat ctgtaatggt taaaactaaa
    12181 atcagcagag gcgttgataa atagcttgtt gccgagttgc tttcatttaa tgtaataaca
    12241 aaccatttcg aaaaaaaatg ttcgaaataa taaatcatta acctgtggtt tgctgaggtt
    12301 aactaggctt taattacaaa ttttaatacg gtgtacattg tttgatttaa agctgatgag
    12361 gtgacaaata taagaaaaat atgaatataa accaggttgg gcctgtttgc tatgtaatgg
    12421 ttaaaaaata gtgtttttag accatttttt aaaagttggt gtttaacaac taaccttact
    12481 tttgttaaca tacaacaact aaatactaaa taacatgaaa aatatgtatt tacaattttg
    12541 ttttttatta aaaacaaaag ctatgctcgt attgcttttg ctcaatacgt actttgcatt
    12601 atgtagttca gcacaaacaa tcccgctggt ttatgatgtt gaaaatacag gagatacttt
    12661 tgctaagcct gttttgccaa caaaagacca actacctgta gttgaaccat tgacagaccc
    12721 ttttatgtgg tctgatggta gcggccgaag cactaatttc agcgattgga gccatcgtcg
    12781 cgccgaaatt gccgctgaaa tacagcacta tgagattggg gcaaaaccta aacgcccgga
    12841 tacaataact gctacttatt ctggaggggt tctgactgta gttgttacag aaaatgggaa
    12901 aacgctaacc ttgaaatcgc aagttgtttt acccggcggc actggcccgt tcccggctgt
    12961 aattggtatg aacagcggga caggaagcct tcctcccagc ttgtttacca ataatggtat
    13021 tgcaacaatt acttacaacc ataatgatgt tactacatac aatggtaaat ctgcaacaaa
    13081 cccttatttt cagatgtacc cggatcttat ttatgcaggg caatatagtg cctgggcttg
    13141 gggggtaagc cgtcttattg acgggttaga attggttcaa aacactttaa acattgattt
    13201 aaaacatttg gctgttaccg gatgctctta tgctggtaag atggcccttt ttgccggggc
    13261 tttagatgaa cgtatagccc ttactattcc tcaggaatct ggaggtggcg gagctgctgc
    13321 atggcgtgtt tctcaatctt taaccgaaag tgtagaaacg ttgagtgcaa caaactattc
    13381 ctggtttatg caatcaatgg gccaatttgg aggggcaagt gtagccaagc tccctcatga
    13441 tcatcacgaa ctgttggctt tagtggctcc ccgggcttta ttagtattgg ggaataatga
    13501 tatgatatgg ttggcagatg agtctggata cgtttcctgc agagctgccc aaaaagtata
    13561 tgaaaccttt ggtattgccg acagatttgg tttttcaatt gtcggagggc atggccattg
    13621 cgctttgcca tcagggcaat atcctgaagt gcttgctttt attaaaaaat ttcttcttgg
    13681 tattgataca ataaatacaa atgtaaggat aagcccatat cagtatgttg attatgccag
    13741 atggacagaa tggtggggta caggtgttgc tgaatttcct gttatcgaag gagaaaaatt
    13801 gtatgaagcc gaatgtgcta ttgttggaaa taattgggaa tccgtaagtt acagtaaatg
    13861 ttcaaataaa acttatttaa aagccaaaac agggataagc agtacgtttg tggctcccac
    13921 agacagcgca aatattgttt ttgtgccaat tacgatagat aaagattcta cttactatat
    13981 ttttgcccat gtaaattgtc cctcgggcaa tgctgattct tactgggtta aagttgatga
    14041 tagttcattt gtcagtgttg atggcctttc tacttctgac tggaaatgga gatatttgaa
    14101 tagttataaa ttaacagccg ggcaacatac tcttgccatt gggattaatg aaaatgggct
    14161 ttgcttgata aaatatccat caccccacaa ccatttcagc catcaggctt gggcaaaaca
    14221 gctgtcaata attgtgggta taatgaattt ttttatattg agcctgaatg tgccacaaaa
    14281 ggtaaggatt ggcgagtagg ttatgataca ttagcctcaa acaatggtta tatagtagta
    14341 aggcctgata ccgaatctgt tgctgctgcc cctgcaacta aggctggaga agtgctttta
    14401 aaatttaacc ctacacagga agcaatatat tatgtttatg gacgtgttaa ctgtccaagc
    14461 gctaacgatg attcatattg ggttaagttt gataatgaag actatcagat ggtaaatggc
    14521 ttgacaactt ctggatggga atggaaagtt ttagcaagcg atacattaac tgccggaact
    14581 cataacttta ctattgctta ccgcgaaaat ggagctaaat tggataaggt ttgtattaca
    14641 aattcttcca tagtcccgag tggtatgggg gataaagcct ctggcagttg taaagttaca
    14701 gatattgaaa tggtcaatat tgatggatat tcattaggcc aaaattatcc caatcctgtt
    14761 attggacaaa ctaacattgc ttttgaaatt ccaaaaagtg catttgtatc tttaaaagtg
    14821 tttaatttac ttggggttca aattgctgaa ttagcaggta aaacatatcc tcagggcaaa
    14881 catgttgtat catttgatgc tcatggtatt cctaatggaa tgtattatta taccatcatg
    14941 gccaatgaat atacttgccg taaaatgatg ttaaaacaaa agtaaacgat aaataaattt
    15001 tatagttaaa ttttaatgta aggtttttgg gatggccgct gttagaaaaa acagtggcca
    15061 tcttttatag cacttatgtt tgataaaata taaatgttat taaccccaac ttaactttat
    15121 taaacctgtc aacaaacaac agtcaaccaa agcgctatat ttcttttgtt tgtaaaatgg
    15181 caaagttgta tatttgttgc ccttaagatt tcgactaatg gagtggtttc attggataga
    15241 gctttttatt aaaggtttgg tgttggggta tattacctct ataccccttg gccccattgg
    15301 tattattgtt gtacagcgga cgttgagcaa agggaaactg tcaggctttg tttccgggct
    15361 tggtgcggct tctgtagata catttcttgc cgttattgca gggctggggc tttcgtttat
    15421 tattgatttt attgaagatt atagtatcct gttcaagctt tttggcggat taatcatcat
    15481 ttttattggt ggcagaatat ttttcaaaag tccggtacgt caggtccgtg agcgtaagca
    15541 aaaaaagtcg agcctgcata ccgattattt atcggtagta gcgttaacat tgtcaaatcc
    15601 gttggccgta tttttgttta tagctatttt tgcagggctt aatgttgtta acggggttaa
    15661 aagctttgtc agcctggggt ttgtatttac cggcatattt ataggcgcat cactatggtg
    15721 gttgacactt acatcttttg ttaatattta tcgagacaag ttcaggttga aaaacctttg
    15781 gtggttgaac aaaattaccg gggtactcat tgtaatactg ggattagcta cacttatcag
    15841 tattttattt aattaattta tattaaatga atcagttgta tcctattcgt ttcaggccga
    15901 tattgaaaga tatgatatgg ggaggttcta aactccgtaa aatttttggc aaacctggag
    15961 cgaccgacaa aagtggcgaa agctgggaaa tatcagatgt aaaaaataac agctcggtag
    16021 tcgaaaatgg ttttttagcg ggtaataccc tgggtgagat tgccgaaatt tacatgggcg
    16081 atttgttggg cgacaaagta tatgaacgtt ttggacaaca attccctttg ttgataaaat
    16141 atattgatgc caacgataag ctctctattc aagtacatcc ggatgacaaa atggcttatg
    16201 agaagcataa aagctatggc aaatcagaga tgtggtatgt tgtgcaagcc gaacccggcg
    16261 caaagcttat tgttgggttt aataaaacta tagatgcaga tacctgtctt caacatttga
    16321 atagcaatac gttacctgaa attctcaatt ttgaagatgt taaagctggc gatgtttttt
    16381 atttacctgc cggtcgcata catgccattg gagaaggctt gcttattgcc gaaattcagc
    16441 agacttcaga tattacttat cggattttcg actggaaccg cactgatgct cagggtaaac
    16501 ctcgagaact gcatacagaa cttgctctgc aggctataga ctttagccct gtgaaggatt
    16561 tcaaaacatc ttacaagaac aagctcaacc atactgttga attggtaaaa tgcgagtact
    16621 ttaccacaaa ccttatagaa cttgaccaaa cattggaaaa agattacgaa agtattgatt
    16681 catttgttat ttatatgtgt atagaaggtc aggtaattgt aaaaccatac gatcaagata
    16741 gtataacaat aaaagctggt gaaactattc taattccggc tgttttgaaa aatattatta
    16801 ttgaacccct tactacgacg aagatattgg aagtttatat tgattaaaaa aatatcatgt
    16861 caataaaaat tacaagtctg aatgttgaat ttttcaagag ctgtccttca ctgaaaggct
    16921 gccctgaggg gcaatatcct gaatttgctt ttattggcag gtcaaatgtt ggtaaatcat
    16981 cattaataaa tatgttggcc aacaaccgta aacttgccca tacatccggc cagccaggaa
    17041 aaacaaagct tatcaattat tatctggcta catttaagga acaggtcggt aatgaagaaa
    17101 atataagaaa aatcccctgg tttttagttg acctgccggg ctatggcttt gccaaaacat
    17161 ctaaggccca acgcggtgaa tttagcagta ttatttcaga ttatgttttc aaacgccaaa
    17221 accttacctg cctgtttgtc cttattgaca gccgtcttga accgcaacaa attgatatta
    17281 aatttattac acttttgggg caacaagggg tgcctttttg tctggtgttt accaaaattg
    17341 ataaaatatc aagcaattca gtcaattcaa atattgaaaa attcaaagca acgcttttgg
    17401 agcaatggga agaatttccc aagatatttt taacttctgc cgaaaaaaaa caagggaggg
    17461 aagaattgct aaattttgta aaagatacca tcaggagcct taaatcataa attgctttta
    17521 taacttatta ataatgagtg tttagtatat ttttttaaaa taatttaaca cacagttata
    17581 agtaatcaat tgaattcata aatttgcggc cagaaataaa caatttccaa tggacagaag
    17641 atctcctctt aaattttttt caggaacagc atcaacatat ctggctgaaa agatatccaa
    17701 gagttatgga acacaattag ggagtaccca gttactccgt tttagcgatg gcgagtttca
    17761 gccttcttac cttgaatcag tacggggatg tactgtattt attatccaat caacatttcc
    17821 acctgccgaa aacctgcttg agcttttatt gatgattgat gcagccaaac gtgcgtcagc
    17881 aaaaagagtg gtggctgtaa taccatattt tggttttgca cggcaggata gaaaagacag
    17941 gcctcgttcg gctatcggag ccaagcttgt ggccaattta ttgacagctg caggtattga
    18001 cagggttatg acaatggact tacatgccga tcagttgcag ggtttttttg atgttcctgt
    18061 agatcacctg tatgcatcat caatatttgt accttatatc aatagtttaa accttgaaaa
    18121 ccttgttgtt gcagctcctg atatgggagg aagcaaaagg gccaatgcat attcacgttt
    18181 tctgaaagca gaaatggtaa tatgttacaa attaagaaaa aaagctaata ccattgaaga
    18241 atttaaggta attggcgatg ttcgtgataa aaacatcatt gttatagatg atatgattga
    18301 tacagccggc actataacag ttgctgctaa tatgatgatg gaagaagggg cacgcagcgt
    18361 cagggttgtt gcaacacatc ctgtactttc gggcccggca tacgaacgta ttgaaaatag
    18421 taagattacc gaagttatgg taacggatac tatacctctg aagcaacaaa gccccaaaat
    18481 caaggtaatt tctattgccg acatttttgc cgatactata aataaagttt ataactatca
    18541 gtcaattagt ggcaatttta taatgtaaaa aaatatatat ttgcactccc tgtttacgac
    18601 ctgtaaaaag gttgtagtgt aatggggaat taacaataaa ttagttagtt acagttaatt
    18661 ctgagtagca catttaaaaa tttaattatg aaagtattcg aattagaagg aacaattagg
    18721 ccagaacttg gcaaaaaatc tgcagccaat ttacgtaaac aagacagcgt accttgcgta
    18781 ctttatggtg gaaaagagaa tgtaaatttc tttgttacaa accctgctat acgcgacctt
    18841 atttacactc ctaacgttta cattgtaaac cttaagttgg gaaaaattac caaacaagct
    18901 gttatgcgtg aaattcagtt tcatcctgtt acagacaaag tgttgcatgt tgatttttat
    18961 gaaattgcag atgataaaga agtagcaatg gaaattccta tccgtattgt tggtaactca
    19021 gttggggtaa gaaaaggggg aaagatcaaa caggcaaaac gaatgttgaa agtaaaagca
    19081 ttgcctaaaa acctgccaga tattcttgaa gtggacatta ccaatcttga tgttgagcaa
    19141 acgattaaag tggcagatct taaatatgaa aacctggtaa tgctaaatgg cctgcgcgaa
    19201 ccggttgtta gtgtaatggc ttcacgtgct actgctaaag ccgaagctgc tgctaagtaa
    19261 tgtaataaaa attttgtagt ttgaaatatt tagttgcggg tttgggcaat ataggaagtg
    19321 agtacgctca tacccgtcat aatataggtt ttgatgtatt ggatgctctt gccggggcgt
    19381 ccaatattta ttttgaggat ggccgatatg cgtttaatac ccggttttct atcaagggaa
    19441 ggacgattgt ccttataaaa cctactactt atatgaacct tagcggcaaa gctgttagtt
    19501 actggctgca aaaagaaaat atccccgtag aaaacctgtt ggtagtagtt gatgatttgg
    19561 cacttccggt tggggccata cgtattaggc ccaaaggtgg cgatggcgga cacaatgggt
    19621 tgaaaaatat tcaggaaatg ctgggacatg ctaattatgc ccgtcttcgt tttggtattg
    19681 ggagcgaatt tgcaaaaggc cgtcaggtgg actttgtcct tggcaaatgg gcagaagaag
    19741 aaaaagcatt gataaaaccg gctatcgaaa ccagttgtga agtaattaaa agctttgtcc
    19801 ttgccggcct tgaaaggact atgacttcct tcaataagta attttctttt gtaagaatct
    19861 tttataacct atatatttac taaaataatg taggtttgat gaagataatt ggacccttta
    19921 aagtacttct aattgtagca cttatctgtg ctgatttttt aaaagcccag gattctttaa
    19981 attatgtaaa acctcctaaa aagtggggtt taggtatcgt tcctgctgta gcttataata
    20041 ctgactgggg ctttcagtat ggtgtagctg gaaatcttta taattttgga aaaacatacc
    20101 cggattataa atatacttat tatttcgagt ggtcgagaac aacaagaggc aatagcgtta
    20161 atgaaatatt ttttgatcaa aagttttttt tattaaaaat cttcgattga catacaatgt
    20221 tggttatttt ttcaataaac aattgccttt ctatggtttt gacggataca atgccagata
    20281 tctgaaggaa aatgaagacc aaaataatac aacctttgcc agcaggatgt attacaggct
    20341 cgacagggct gtaataaggt gtttgttgga ttttcaatat aaaacggcat ataatccttt
    20401 acgcatttta gctggtgttg gttattttga tacaaggata aagcctgtgg atattgataa
    20461 gtttaatgaa tcgcttgacg acgatgataa aatactgaaa aatactacgc ttttcgaaaa
    20521 gtatgtgtcc aatggtatta tttctgagga tgaaaaagat ggtggcaaat gctttatact
    20581 taaatcaggg gtaatatacg atacccggga taacgaaacc aatgccaaca gggggatttg
    20641 ggcagaggtg cttttgattt cagctccaaa gccgggcaat aatacttcat ttactcaagt
    20701 gggggctgtt ttcaggcatt attttaccat atcggctccc aggttgacca tggccaatcg
    20761 tattggaatt caaactttag tagccggcca gaagcccttt tacatgttac cgtacttatt
    20821 tagttcttat aggttgaggg atgcttttgg aggcgaaaaa actatacgtg gagtattggc
    20881 acagcgtatt cagggcaatg gtgttgcttt tgggaacttc gagctgaggt ataaatttct
    20941 ttatgggcgt ttatttaagc aggagactta tttgtgtgcc aatgtttttg ccgactgggg
    21001 aatggttaca agcaaatatc tggataaacc agtaccaatg gctttagaaa atgaaataaa
    21061 acctgggcag gaaaaacccc actggactac aggtgcaggc ttgcgtgttg tgatgaacca
    21121 gaattttgtg gtcacagctg attatggcag ggcacttgac ctgcaggatg gaaaaaacgc
    21181 tttgtatatt accctgggtt ttttgtatta ggttaataac aaaaaagcag cgccccttcc
    21241 gaaacgctgc tttgtatatt gtgaaagggt gtttttacaa cagtgcatta atcttgttta
    21301 ccaatgtgct ttttggaaca gcaccaactt gtttgtccac tacttcgcca tttttaaaaa
    21361 ataaaatggt gggaatgttg cggatactgt atttcatggc aatggcattg ttgttgtcaa
    21421 cgtccacttt gcctactaca attttatcag ccatttcgtg cccaatttct tctacaatag
    21481 ggccaaccat gcggcacggg ccacaccatt cggcccaaaa gtcgaccatt acgggcttgt
    21541 ccgatttgag gactacctgt tcaaaatttc cttcagttat ttcaagtgcc ataattataa
    21601 tgttttaaat gtgaatgctt tagttgtgta aaaccgaaaa tttgatccca ttgtttaagt
    21661 gtataaattt aatcagatta tctgaaatat ttattttgcc cattcttgaa ataagatcta
    21721 ccatcatatt ttcttcaggg tcaattaacg aaacttttaa caatgttttg ccatcttttg
    21781 tttcaagctt ttgtgttaat tcactgacaa aggtttcgtc aatatacgaa gaagggatgc
    21841 gcaaatgcaa cgacttgaca aactcatcgc gtacactgct gaggtggata atttttgaga
    21901 tgttaaattc cagttcattc tcgttatatt tgcgcgtttg cactgttcct ttgataagca
    21961 agccgatatt ttttatacag actttataat agttttcaaa gtctttgcca aaaagctgga
    22021 aacggaatga ccctgaaaaa tcctcgatag ttaccgaggc ataattttta ttagtttttg
    22081 ttatgccatc gcggatatct gtaacaagcc ccatcacaat aatttcttta cctttcaact
    22141 ttgataaatc tgacaattca gaaagaggat gagtacagat gttttcaaat tcaaacctgt
    22201 aattgtccaa cgggtgtgca gaaagataca tacccacaag ctctttttcg cgttttaact
    22261 gctccagtag tgtccatggc tctacggatg gtatctgagg cttagttgta ctgatatggt
    22321 ttgctattcc tgcatcacca aagagagtag cggtattctg tgctttgtct gcttggaaaa
    22381 ggttgccata tcgcatgaga gtgtccagaa atacgccttg ctggtcgggt gcaaaaaatg
    22441 ctgaacgttg gatgccaaat aaatcaaagg ccccggccaa tatgatattt tcaacagcct
    22501 tacggttaac cgaagacagg ttcatccgtt caaaaaaatc atacacgtct ttaaaagcgc
    22561 catttttctc gcgttcttca acaatagctg ttactgcatt ttcgccaagt ccttttatag
    22621 aacctaagcc aaatcgaatg tctccttttt tattaacagt aaagatgtgt ttactttcat
    22681 tgatatccgg gccaaggact tgtatgccaa ttcttcggca ctcgtccatg aaaaatgtaa
    22741 ttttatcaat actacttgca ttgtttaata ctgaagccat gtattcggcc ggataatgag
    22801 cttttaggta tgcagtctgg aatgcaacaa aggcataaca tgtactgtgc gacttattga
    22861 aagcatacga agcaaaagct tcccagtctg tccatatttt ttcaagcttt tcggggtcgt
    22921 gacctttggc tgtagcattt gcaataaact ggggcttaag tttgtccagc gttttacggt
    22981 cctttttacc catagctttg cgtaagacat cggcatcgcc ctttgtaaag cctgctaatt
    23041 tctgagaaag tagcatcacc tgctcttgat atacagtaat accataagta tctttgaggt
    23101 attcttccat ttccggcaaa tcgtaagata cgggttcgcg tccgtgttta cgggcaatga
    23161 aattggggat atattcgata ggcccggggc ggtacaatgc gttcatggct atgaggtcgc
    23221 caaacttgtc gggctttagc tctcgcaaat atttttgcat cccggggctt tcaaactgga
    23281 acgtgccgtt tgtttcgcct ttttggtata gttcgaatgt tttggcatca tccaggggaa
    23341 tagcatcaat atctatatca acaccgtggt tttgcttaat aatttcaagt gctcctttaa
    23401 tgatggtaag ggttttgagc ccaagaaagt ccatttttat aactccggct tcttctatta
    23461 ctttgccttc atattgggtt acaagtagtt cggagtcttt agcaactgcg acaggtaaaa
    23521 ggtctgataa atcttgtggt gcaataataa taccgcaggc atgtacgcct gtgttgcgga
    23581 cagagccttc aagcaaacgg gcttctttca aaacctgagc ttgtaagtca ttgccgctga
    23641 ttatttcgcg caaacgcttt acattgttca aatcatcagc attaagccct tctttttccg
    23701 aaaggctttt ttcgccggtc aatggggctt caaaaaccct gttaagttca attcctggag
    23761 tatcggggac taacttggtc agttcaaggc tttcgttgag cggcaggtca agtacacggg
    23821 caacgtcctt gatactcatt ttggcagcca tggtgccata agtgacaatc tgtgcaacct
    23881 ggttttgccc gtatttcttc acaacatagt caattacctt ctgacggcct tcatcgtcca
    23941 tgtcggtatc aatatcgggc atgctctttc ggtcgggatt gaggaaacgc tcgaacagca
    24001 ggtcgtactt aatggggtcg atgttagtaa tgtcgataca atatgcaact gccgatccgg
    24061 cagccgaacc acgtccaggc ccaatgaaaa cacccatttc gcgcccggct ttgataaaat
    24121 ccattacaat aaggaagtat cctgcaaaac ccatattttc aatggtttgc aactcaaaat
    24181 tgagacgttc ttctacttct ttggtaattg taccatattt tcgtttggcc ccttcaaagg
    24241 taaggtgcct caaatattgc atttgagtca ggaaattggg cggaataggg aagttgggca
    24301 gcaggatatc ccttttcaaa tcgagatatt caaccttgtc aactatatca ttagtattgt
    24361 caatggcttg cggaaggtca gcaaagagct ggctcatttc ctgcgtagtc ttgaagtaaa
    24421 attggtcatt gtaaaacccg aagcgcgagt tcttttttgc accttcttca tcttcaaggt
    24481 attcttttac aataggggta ctcttttttt cgccggtatt gatacaaagc aggatgtcgt
    24541 gggcattgta gtctgaaaca tctacgtaat gagaatcgtt agtagcaatc accttaacat
    24601 tgtatttgtc ggccagtttt ttcagcacat cgtttacttt atcctgatcg gggatgccat
    24661 gacgttggag ttcaacgtaa taatcttctc caaaaatatt ataccaccac ttgaattctt
    24721 cttcaccggc ttcttctcct cttttcagga tggttttggg taccgatgcg ccaaggcaac
    24781 aagtagtagc aataagccct tcgtgatatt tttcaatgag ttctttgtca atacgagggt
    24841 atttaccata caatccttct atatatccca aagaacaaag ctttgaaagg tttcggtaac
    24901 cctgctcgtt tttggcaagt aatacctggt gatatcgaat gtctttttct tctcgtgtaa
    24961 attggcgttt aaagcggttt tcgacaaggt aaaactcgca gcctacaatt ggctttacct
    25021 tgatgcgctt gtcagtttta tcatcggggt ttactttgtg tttataagct tctgccacaa
    25081 attcaaatac tccaaacata ttgccatggt ctgttatggc aagtgccggc tggccatcat
    25141 ccatggcttt cttgtagagc ctttttattt cggccgctcc gtcaagcaaa gaaaactgtg
    25201 tatgtacatg taaatgtgaa aaattcataa aaggcttata aataataggt tatatcgtaa
    25261 aacatacaat aacctgtcag tgttcaaaat taggggtaat ctgtgctcaa aaaaagcatt
    25321 gttaataaat ggatgataaa aattactatc gaccaaaagt gaagattttc atatatttgc
    25381 acctcgtggc attttatttg cacgagcaac tatcaggctt taagtgttac tgaaattgtt
    25441 caaagtttat atcttaattc ctggttcttg gcttttgaat cttttaaact gaagttatat
    25501 atggcatttt taaacgaaaa ctatttgaaa ttaaaagccg gatatttgtt tccggaaata
    25561 ggaagacgag ttcgtgaatt tcaaactcag catcctcagg ctgatattat ccgcatgggt
    25621 attggcgatg tgacacggcc tttgactcct gctgttataa aggcatttca tgatgcaatt
    25681 gatgaaatgg cttctgtaca aacatttaag ggttatggcc cagaacaggg gtatgatttt
    25741 cttcgtcagg ccattgttga caacgaatac atctcgcgtg gggcagacat agaaattgac
    25801 gaggtttttg tttcagatgg ttcaaagtgc gatactggta acatccttga tattcttggt
    25861 cgcgacaata ttgtagctat tacagaccca gtatatcctg tatatgttga caccaatgtt
    25921 atggatggcc gtacaggtga ggtacaggat aatggttatt acaaaggtat tgtatatttg
    25981 ccatgtacag aagctaacca ctttgtaccc gagattccta aaacaaaagt ggatcttatt
    26041 tatctttgtt tcccgaataa cccgactggt atgaccattg gtaaagacga gctcaaacgc
    26101 tgggttgact atgcgcttgc caatgacgct attattttat atgacgcagc ttacgaagca
    26161 tatatctctg ataaagctat tcctcacact atttttgaga ttgaaggtgc acgtaactgt
    26221 gcgatagaat tccgcagttt ttcaaaaact gccgggttta ccggaacacg ttgtgcttat
    26281 actatcgttc ctaagacttt gatggcaaaa gatggcaaag gtcagaaata cccattgaac
    26341 cagctttggt tacgtcgtca aactaccaag tttaacggtg tttcatatcc tgttcaaaaa
    26401 gcagctgcag ccatttacac cggacaaggc aaaaaagaaa cagccgaaac cattaaatac
    26461 tatatggaga atgctgctat catgcgtaaa agcctttctg atgaggggta taaagtattc
    26521 ggtggtgtca atgcccctta tgtatgggta aaaacaaaga acaacatgtc ttcatgggat
    26581 ttcttcgatt tattgcttgg caaagccaat gtagtaggta ctcccggctc tggctttggc
    26641 ccatcaggcg aaggatattt ccgcttttct gcttttgccg acaggcagaa agtattggaa
    26701 gccatggaac gcatcaaaaa acttggttaa tcgatattta taaggatatg cgcattggtt
    26761 ttgatgcaaa gcgggcattt ttcaacaaaa gtgggttagg gacctacagc agggaaacta
    26821 tatccatttt gtccgatttt ttcccaacca atgagtatat cctttattct cctaggcctt
    26881 caaaaggtaa aatatttgaa aagactgaca atttaacggt ttcttaccct cagacttttt
    26941 attataggac attttctcat ttgtggaggg cttatggttt gtcaggacag ttggttaaag
    27001 aaaaaattga cctttatcac gggttgagca atgagttgcc gatgaatatc catcaatacc
    27061 cagtgaaaac agtagtgaca atccatgatt tgattttttt gcgttatcca aagctttatg
    27121 gatattacga ccgcaagaac tacgaaaaga aatttcgtta tgcagcgcag tctgccaaca
    27181 gggttattgc tgtaagccgg caaacggcga acgatttgtt tgaatttttt tctattgatg
    27241 aaaaaaaggt tgatgtagtt taccagggtt gtaaccctca gttttggcag attgtttctg
    27301 aagcggataa acagggaaca gcttcgaaat acttaattcc tgaaaagttt atcctttcag
    27361 taggtactat tgaaaaaagg aagaatatat tggctgtaat caaggccatg caccagcata
    27421 aaatttttta ccctcttgtt gttgttggcc gtccaacgga ttatcttgac gaaatacgca
    27481 attatattga agagcataat ttaaacaata tctacttttt gcacgatgtg ccaattgatg
    27541 atttgccagc attttatcag atggcagagc tgtttatcta tccttcgctt tttgaaggtt
    27601 ttggaatccc cattgtagaa gcgctttgtt caaaagttcc tgtaattacc agtaaaggag
    27661 ggtgttttgc tgaggctggc ggtaaatcaa gcatttatat tgacccttac aatattgacg
    27721 aaattgggga ggctataaaa tcggtattga ataataatgc acttcgtata tctatgatat
    27781 cagaagggta caaccatgtg caacagtttc gtctggaaaa tattgccgga aacattatgc
    27841 gtacttacga aaagattttg taactgccaa atccaattga tgctggataa aatcactaat
    27901 ggtttattta cattccttta cccttctgcg gtaaaaaaaa taaagtgttc agaactatcg
    27961 ttattcctta cttttgatga tgggccaact ccggaagtta cttctcaggt gcttaaaatt
    28021 ttggcagctt ataatgctaa ggctacattc ttttgtctcg gaaaacaagt cgaagctcat
    28081 cccgggctgt ttaaaagtat tgtagaacaa ggccatgctg taggtattca tggatatttg
    28141 catatttcag gcatttcaat tactaaaagt gcttttgcag aaaacattga gcagtgtaac
    28201 aggcttattt catcagattt agtgcgccct ccgtatggac gcatgacttt aagccagtac
    28261 ttatggttaa taaaacggta taagcttgtt ctttgggatg ttatggctta cgattttaaa
    28321 aaagaaatat ctgtagacac tgttgttgct aatgtttgta ataatgtgcg aaatggttca
    28381 ataatagttt tgcatgatac agaaagaaca aaggatattc tggtttctgc attgcctatt
    28441 atgcttgata aattaaaggg ctattctttt aaaaaaattg acacatttta ataaaatacc
    28501 ccatttattc tttcttcgtg aactttgcgg ttaatatttc tttttagttc atatgtttgt
    28561 aaagtcaagc tttggtcaaa agaaaattaa ccttgataag caaataaaac agttgtcaca
    28621 aacagaacga tataaagaca atataagttt attgctttct gtgccgtcta ttggcataac
    28681 ctcggcaatg atatttttga ccgaaattgg agatataaaa cgttttgaaa ggtttgacga
    28741 gctttgtagc ttttttggct taatacccaa ttgtcatagc agtggcgaca acgaaagagt
    28801 tgggcatata acaagaagag gaaaccaaat ttttaaagtc aatactggta gaatgcgctt
    28861 gggttgctgt aagaaaagac cctgcattgt tgcaatgtta taaagaactt ctaccaagaa
    28921 tggaggccaa taaagcaata gtaaagattg caaaaaggct tttaagtagg ataaggcatg
    28981 tacttataaa aaaagaaaaa tatcaattag gaatattaca gtaacctgta aatttatttg
    29041 aagcaggaaa aacgctagag ggtgtatgca gcaattgact gctattttta gtttgcttcc
    29101 tgctcctata aaatatgata agagaacttg cgacaaaaga tcgctaatag aagattggtt
    29161 tataggttaa attataagga attaagctat ttaattttta aacagcggcc acccttatgg
    29221 gacattttag ggtccccgga tggccgccgt ttaaaaaaaa gcaactattt tgaccagtgg
    29281 tttgttttac aacatagaac acaccctctt gtttttctaa atattttaaa gtgatttcga
    29341 tataagaata acatcgtcaa ttattatttg gaaaatattg aacagcagct cgctttgctc
    29401 cggacaacgg cctgagcaaa gcgagctgct gcccggagcc atcgataaaa ccttttttag
    29461 ccaaaatacg acaaccttca gtttttgaaa cttttgcttc aaagcaaaaa tttgcaatgc
    29521 ttgaaaatat caaacacata agcatttttc ccatgtcgaa ccgactttaa cttagtaaca
    29581 ttttttaata aataccagca cctaaatttg aaaatatgca agctaaggca aagtataaga
    29641 atggaaaaaa agctattttt gccaaaattt tttaaatgaa cagaaaaaaa gctgaaaaaa
    29701 acattactga ccaagaattg gcaaatgcta ttatcgatgc aatccaggaa ataaaaggca
    29761 aagagattgt taagatagac cttcaaaaaa ctggaaatgc aatatgcgat ttctttatta
    29821 tttgtcaggc agattctacc acccatgtca atgctatagc agaaaatatt gaaaaaactt
    29881 tgtgggaaca aaacaaagta tatcctcacc atgttgaagg aaaagaaaat ggtctttggg
    29941 tcctattaga tttttacaat gtaatagttc atgtatttca acaagaatac cggaactttt
    30001 ataagttaga agacctttgg gctgatggaa aaattgaaaa aattaaagaa ccagtaaaag
    30061 taacggcatg aacaacgcaa aaaacaaaga taagcaacaa gagaagccta aaaaagaaaa
    30121 aaaacaaggc ttaggtggcg aaaatcttcc caaacctaaa attaaccctt ttggggttta
    30181 tggtttaatt gcagcagcat ttataggtat ccagtttttt attagcagct cgggcccgca
    30241 agaaatcttt atgaaagatt tcaaaactat gataactgct aaagacgtgg acaaaatcga
    30301 aataatcaac aaagaaacag cacgcattac actaaaaaaa gacagattag acaaatatca
    30361 aaaaatagta ggccgaaatt tttcaaaaat ccccaaagaa ggcccgcact ttgagatgca
    30421 aattggcgac caggcttctt ttgaaaacag tttgaaagaa tttcaggcag acttccctga
    30481 caatgaaaaa atttacgctt cgagcaaaac cgaaaaaaat tatctttttg aaattcttat
    30541 cagctttgta ttgcccatag ttatcctcat tgccctttgg ggatttttgt acaaaagaat
    30601 gaccggaggc accggagctg ggccaggagg agccaacata tttaatgttg gcaagtcgaa
    30661 agcaaaaatc ttcgacaaag aagccagcat tcaaatcaac tttaatgatg ttgcagggtt
    30721 ggaagaagct aaagtagagc tcaaagaaat tgttgacttt ctaaagaacc ctaagaaata
    30781 taccgaactc ggggggaaaa tcccacgcgg tgcgttgctt gtaggccctc cggggacagg
    30841 taaaactttg cttgcaaaag ctattgcagg agaagcaaat gtaccatttt tctccatgtc
    30901 gggatctgac tttgtagaga tgtttgtcgg tgtaggagca tcacgcgttc gtgacctttt
    30961 tgcacaagca aaagcaaaag ctccatgcat tgttttcatt gacgaaattg atgctgtagg
    31021 tcgtgccaga ggcagaagtg gaggttttgc cgctaatgac gaacgtgaaa atacgctcaa
    31081 ccagttgctt accgagatgg atggttttgc cccaaacaca ggtgttatta tccttgctgc
    31141 tactaaccgt gccgatgtgc ttgaccgtgc attgctgcgc gcaggacgtt ttgacagaca
    31201 gatacacgtt gaactgcctg atttgaaaga acgcctggaa atatttaaag tacacatgaa
    31261 acccctcaaa ttagcagatg atattgacat tgattttttt gcaaaacaaa caccaggctt
    31321 ctccggagca gatatttcca atgtatgtaa cgaagctgca cttgttgctg cccgaaaaga
    31381 taaaaaagcc gttgaaaaac aggatttcct tgatgctata gaccgcatca taggcggact
    31441 tgagaaacgc aacaaaatag tttctaagtt ggaaaagaaa accatcgcat ttcacgaagc
    31501 tggccatgca actgtcagtt ggttgctcga acatgccaat ccgttggtga aagttactat
    31561 tatccctcga ggcagggcct taggtgcagc ctggtatctg ccagaagaaa gacaaatcac
    31621 tacccgtgaa caacttttgg atgaaatgtg ctcagcttta ggcggcagag catcagaaga
    31681 tatagtattt ggaaaagttt cgacaggtgc ccttaacgac ctcgaacgag ttaccaaaca
    31741 atcgtatgcc atggtctcat actttgggat gagcccaaag attggcaatt taagttttta
    31801 cgattcatca ggacagagcg aatttacttt tggaaaacca tatagcgaaa aaacagccga
    31861 acttattgat atagaagcta aaaacattgt agaagagcaa tatcaacgcg ctaaacagat
    31921 tttgactgaa aaccgtgagg gcctaaacca gcttgcacag atattacttg acaaagaagt
    31981 gttgtttagt gatgatttag aaaatatctt tggcccacga aaaggtggaa ttccggaaga
    32041 acatcagctt cttatcaaaa atggtaaaac agagaatgaa acaaaagaag gtaacaaacc
    32101 cgaaccaacc gaaaataaag accaacaagc ataatggaac aaggatgggt taaaatatac
    32161 aaaacagata aagattatca agcagatatt gttatcgaac tacttgatga aaataccatt
    32221 aaggctgtta agataaacaa gcgcgactca tcataccagg cttttggcaa tatcgaagta
    32281 tatgtaaaag aagaaaacac tgaaaaagcg ttacaaataa ttaaaaaatc tgcactgtga
    32341 gcgaaattgg gaaacgtaca atatccgggg gcatccttgt tgctgtaatg cttttatgca
    32401 ttcttggagg ttccatttct tttctggcat tctttttact tttaatcatc ggggcattat
    32461 gggaatttta cggcatgttc tttgttgtta atattaaacc ccaacgatgg tctggtatcg
    32521 ttattacagg catcttgttt cttttgtcat tctttatctc ggcaggcctt atttcttcag
    32581 aatggtatct actggtaatt cccagctttt tatacatagt tatcagcgag ttattcaggg
    32641 taaacgataa accttttgaa aacattgcag caactgtgct tggtattatt tatattggcc
    32701 tgcctgtatg ccttttatgg cccattgcat acagggacaa catttaccag tttaattcgc
    32761 atcttgtact tggctatttt attcttatct ggacttatga cacctttgcc taccttatag
    32821 gcaaacaatt tggcaaaagg cgcctgttag aacgagtatc tcccaaaaaa tcatgggaag
    32881 gggcaattgg cggacttctg gtttcttttg ttacagcagt tgttatagct cattttttta
    32941 ccgatttatc cttatttcag tggttagtat ttgctcttat cacgacaata tttggcacac
    33001 tgggagattt gtccgaaagc atgctcaaac gcagcatcgg agtgaaagac tcaggcaata
    33061 tattgccagg acacggagga gtaatggaca gatttgatgc cttatttttg gctatcccgg
    33121 cactttatat atatttgcaa ttgatttaac attttgtata aatgacaatc aaagagctga
    33181 tagtaaatac tactaaacta gcccaaaaaa agttcaggct acatcctgaa ggaaaaagca
    33241 ttattgccaa tatgcttagt tggttattga ttgcaaatgg gatcttattt ttcctttcta
    33301 taagcaatac aatattgacc atcatttaca ttttaagtat tgcggctttc ctttctctta
    33361 tttattattt cagaaaccct gaaagaaaag ttataccaga cgaaaaatat atttatgccc
    33421 cggttgatgg aagaataacc tctgttacag aagttgttga accggaatac tttaaaaaaa
    33481 aatttgtcag ggttgaaata tcattgtctt taaccgatat tcatgtatta cgggtaccac
    33541 ttagtggcaa agttaaatat tgtcattttc agcttgaaga ttaccgtata gcatggcacc
    33601 agctattgtc acacaaagaa agtgcctcag ttggccttat ttcagatacc ggcactgaaa
    33661 tatttttaaa acaaacaggt ggggcaattg tatcttatgc caacgaaaat aattatatta
    33721 accagggcaa tgatctcggt tttataatat ggggctcaag tgttgattta tttatacctt
    33781 taagtgctga tatccgtgtt aaagccggag aacgggtcgt aggcaatatt acaatactgg
    33841 caaaagctac atcataaacg cccataaaac gagcatgata cgttaaacac aaacaaggat
    33901 gaaaattcat catgcgagtt ccatccgacc taattaaaac tttatatacg gacaaaataa
    33961 agttttgagc atcagttagc tttaactaac tttttaatat ttaaaccata aaacgagcat
    34021 gttttatcaa acgcaacata gcaaagcact ctaaacaaaa tgaatattca cgataataat
    34081 aaaattgcca acacaggtgt tccatccatt cacattaaat ggcaaatatt gacagcctaa
    34141 ataattagat ttgaacaaat taaaaactct tacgaatgaa aaaagactac aatacctcgc
    34201 gaaaaaaatt agcattgcca gaatatggca gacatataca tttattagtt gaccacatta
    34261 aaaatattgc tgatagagat gaacggaacc gagcagccca agaattaatc aacattatgg
    34321 gcaatctcaa cccgcaatta cgcgatgtgg ctgattttaa acataaactt tgggatcatt
    34381 tatacgtaat tgccaatttt gaattagata tagactcgcc ttatcctgtt cccactcgag
    34441 aatctttaaa cactaaaccc cgcaaggtat cttatcccca aacctccatc aaatacaagc
    34501 attatggcaa aattactgaa caaatagcta aaagtgttgc caattttgaa gaaggggcaa
    34561 tgaaagatgc cttgatagaa cagcttgcca atattatgaa aaaacaatac ctcacctgga
    34621 accgcgaatc ggttaatgat gaaagtattt tcaaagattt atcagagctt acaaaaggaa
    34681 aaataaatat ccacccggac tctttaaaac tttcagaaac aaaagctatt ctttctcaaa
    34741 aaaataaaat gcacaacaaa aacaaccaac aaaacaacaa aaaccgcaac aatcacaata
    34801 aaaataaaaa caaaaaaaac tgagactaac cctttcattt ttatctgatg gcaatctttg
    34861 aagtacatgg aggaaagcag cttaaaggag ttttaaagcc ccagggtgca aaaaacgaag
    34921 ctttacaggt tatttgtgct tgtctgctca ccgaacaaaa ggtttacatt aataacatcc
    34981 ccgatatccg ggatgttaac atacttatag atatcttaaa gtcacttggt gttgaagttg
    35041 tcaaaactaa agaatacgaa tatagctttg atgcctcaaa agtttacata gactatttaa
    35101 aaacagaaga ctttcgtaaa aaagctgcca gccttagagg ttctattatg attataggcc
    35161 cgttattggc ccgttttggc attggttata tgccaaagcc tggcggcgat atgattggcc
    35221 gccgccgttt ggatactcac tttatcggat ttgaacaact tggtgcaaat ttcgatttcg
    35281 atgccgaaaa taacatatat tctgttaagg cagataaact caaagggaca tatatgttgc
    35341 ttgacgaagc ttctgttacc ggtactgcca atattattat ggcttctgta ctggcaagtg
    35401 gtacaacaac tatatacaac gctgcttgcg aaccatatat acagcaactc tgcaaaatgc
    35461 taaatagtat gggagcaaaa attacaggca taggttcaaa cctgctaaat attgaaggag
    35521 tcaacaactt gcatgggtgt aaccataata tattgcctga tatgattgaa attggcagtt
    35581 ttattggact tgccgccatg acccaatctg aaatcacaat taaagacgtt tcgtacgaaa
    35641 acctgggcat tattcctaat gcctttagcc gattgggaat aacgcttgaa cgaaaaaacg
    35701 atgatatatt tattcctgca caaaaggaat atacaattga tacatttatt gatggttcta
    35761 ttcttactat tgccgatgcc ccatggccag ggcttacccc ggacctgctc agtgtattcc
    35821 ttgtagtagc tacaaaagca aagggcagtg tacttattca ccagaaaatg tttgaaagcc
    35881 gtttgttttt tgttgacaaa ctaatagata tgggtgccca gattatcctt tgcgaccccc
    35941 accgggcaac agtaataggg atgggtaacg aacataaact ccgccctact accatgagct
    36001 ctccagatat cagggcagga gtagcattgc ttattgcagc cttatctgca aatggcaaaa
    36061 gtactattca taacatcgaa caaatagacc gtggatacta caaaatagat gaacggctta
    36121 atgctattgg agctgaaatt atcagaaaat aaaaataatt gaagaattag ccaatagtaa
    36181 tactactgca tttaatccca aattaagcat taaaaatgct taatcgaatc gtctaactga
    36241 cgttagctat aggcttttgc ccaacaagaa aataacacaa aaaaagcccc ggcagaccag
    36301 ggcttttctt atatagtaag ttgttaaaca aacttattct gaaacaatct caaaagctat
    36361 ctcaacgcta acttctttgt gaagttttac tttagcagta tagttgccaa cttctttaat
    36421 gatatcttct ttaataagaa tgtttttcct gtcaacgtta aaaccttgag cagctaaagc
    36481 ttcagcaatt tgaatggtat taaccgaacc aaatattttg ccttttgaac ttgttttagc
    36541 cccaatagta agtttcaaac cttccatttt tttagccaac tgtgtagctt cttcttttac
    36601 tttagcttct ttatgagctc gctgcctgat attttcagca agaattttct tggccgattc
    36661 ggtggcaaca atagccattc cgttaggaat aagatagtta cgagcgtaac cgttttttac
    36721 gtttacgata tcgtctttgc ttcctacgtt tattacttct tgtgtaagta taatttccat
    36781 ggtacaattt cctccaatat tattttaaca tatcggttac ataaggcaat aaagcaaggt
    36841 gacgtgctct tttaatagca gttgccactt tacgctggta ctttaatgaa gttcccgtaa
    36901 ggcgccttgg caatatcttt ccttgttcgt tcaaaaattt ctttaaaaat tctggatctt
    36961 tgtaatcaat atatttgatt ttattttttt tgaaacggca atattttttc tttttgattt
    37021 ctaccgttgg gggcgtcagg tatctaattt ctgattgatt tgttgacata gcttattcct
    37081 ccacttttat aggttctttc atacttcttt ttttgttact gtattcaaca gcatatttat
    37141 ccattgcaaa tgtcaaaaaa cgaattactc gttcatcacg acggaattct gtttctaatt
    37201 ttgcaataag cgcaggctcg gctttaaact ctataaggtt ataaaagccg gtagattttt
    37261 tttgaattgg gtaagccagt tttttcaatc cccaatcttc gctatgtaca atttctgcac
    37321 catgttcggt cagaaaactt ttgaatttgt taaccgcttc cttcatctgt ggctcagaca
    37381 aaacgggagt tacaatgaaa acggtttcat actgttttaa catactcgtt tgtttttaaa
    37441 ttaaaattaa tttggagcgc aaaagtaaaa actttttact gaaaaccaaa agattgcaag
    37501 gtaaaattta gccattactt tattttgcag ggcccttagg atattcaatg cgaaaatggt
    37561 aaatagttct taaacgctgc ttcagcgttt ttttaattat ctcaatatct ctgaaagtaa
    37621 gatcggcatc gaccagttgc tcatcggcaa ttaagccgtt aacaataaca tcaaccatat
    37681 tgttgatttc agcttctgaa tagtgcttaa gactacgggt cgcagcttct acactatcag
    37741 caatcatcac tactgccatt tcgcggctaa atggttttgg gcccgggtat ttaaactgtt
    37801 ttatatctac ctccgtccca ggattttgat ttaagtacga acgataaaag taatgggcca
    37861 gtgatgttcc gtgatgagtt tttataaaat ccgttatttg agcaggaaga tgaaactttt
    37921 cggccagctc aacacctttt gtcacatggc tgatgattat ttttgcactt tcttcaaatg
    37981 caaggctttc atgagggttc acctcatccg aaaggttttc gataaaatat gcagggttgt
    38041 gtattttgcc aatgtcatga taaagagccc ccgcccttac caacaataca tttccgccaa
    38101 tcttaaagat agcttcttcg ctaagattgg caacctgcaa cgaatgctga aatgtacccg
    38161 gcgtaacttc ggccattttg cgaagcagaa tttgattggt atcagacaat tcaagcaatg
    38221 ttgcatctga aacataccca aatatttttt caaatatata tataagcggt aacgccgaaa
    38281 gcattaacag cccattaccg gcaaaataca ataaattatc tgtattgata ttggctaatg
    38341 aaccttcctg atataaagta aggccaacat aagtcaaaga taaggtaagc gtttgccaaa
    38401 tagccgaaaa aaacatacgg tttcgtttgt atgtattgct taaactaaga acaataacag
    38461 caccggccaa tatattaagc ataacaaact cgaagctgtt gggcgcccaa aaaccaaaaa
    38521 gtataataac caaacaatga atataaagtg ccagttttga atcaaaaaaa gtctttaata
    38581 acaccggaat aatagccaaa ggaatcatgt ataacccgcc cggtcggtgt ttaataatca
    38641 aactggttat tagcccaaaa actatgatga tggtataaat aaaagccagg tttttgttcg
    38701 agcgccaaac ttgtcttttc aatttcgaga taaacacatt aataagaaca atgaatgtta
    38761 aaacaataat tgtctggccc aataatataa aaatataatt ggccgagaaa cccatacgct
    38821 gttcgtattc cttttttaca gattctaaaa ctttgtggtg acgcggagta acaatatcac
    38881 cggtaaatat tatcctttcg cccgaaagta tcatgccttg cgtcaacgaa atattagaca
    38941 aggcctgttc tttgaccttt tctgaagtaa cggcatcata aaaaagattg gggatgatat
    39001 atttttcaat ttttaaactt tttaaaaaat taaaaggaga atttccatca gtaaaagcaa
    39061 cctggtcaga aaacttaacc aactcctgaa aaatatgctc ataagcactt ttaggcgtaa
    39121 acaaggtatt agcatcaaca tcttctgcct cattgtctcg tataatagca atagttgatg
    39181 attggtgcaa aatagaagcc ccctcgtccg ggatattgac tataccttta gaatagacag
    39241 tatctaaaag ttccttcaat aactcctcca actgttttct gacaactgtg tctctggccc
    39301 acccttcatt gatatattga agctttttgg taaaagcttg ccaaaactgt tctctctttt
    39361 cctggcctat ttttgggtta aaagtaaaat atggaataaa atgcttagaa atactatctt
    39421 tttcgtacga aagttcttca tcggtcttat atacaggcac atcaaaagga gctacaagca
    39481 cctggtgcat ccatggtttt cctttttgat attcatattt aaactttcct ttatgaggga
    39541 acaaaatgac caataacagg accgaaactg aaaaaataac ccaaaaaacc cgaaaatctt
    39601 cccagtctat attaaatttt atccttttta gcctcaacat gaatgttttt ttatgttttt
    39661 ttcaaaaaac ttatttattt tgaaaaatag gttttaaaaa gtattttacc taaataaacc
    39721 atcaaagaaa caaaaagttt ttcacactaa aaataaacta aaagatgaat attccaacaa
    39781 acgctatttg tacattgccc tacatcgcag taagaaaaga cccttcagaa acttcagaaa
    39841 tgatatcaca gctactattt ggagaaatat ataccgtatc tgacattcag caaaaatggg
    39901 ccaaagtggt gaatcaaacc gacaactaca ccggatggtg cgatgcaaag ctcattaaag
    39961 cgctaagcaa agagcaggtt attgagttgc aaaacatgaa aaaagtggtg gttgggcaaa
    40021 aatggcttga tatacatatc agttgttttg atacttctat tacaatctcg gcaggaagta
    40081 ttctttatca aaaacagcca ggacagtttt ctatcaatgg agagaactgc aatgtatcaa
    40141 caaatgtaat tgagaactat gcaaatacaa tagacacagt aatctatacg gcaaaaagcc
    40201 tattaaacac tccatactta tggggaggac gctcggcata cggcatcgat tgctctggtt
    40261 tggtacaaac atgtttcagg gttgccggaa aaaccttgcc ccgcgatgct tcacaacaaa
    40321 tttacgaagg gaatacggtg agttcacttc aagatgctca aaaaggcgat ttggcttttt
    40381 tctgcaatga aaaaggaagt gtttgccacg taggcattct tttgtccaac aatcagataa
    40441 tccacgcttc gggctttgta aaaattgaca gcattgacat tcagggaatt tttgacaaaa
    40501 acaaagacca atacactcat cacctgtgtg ctattaaaag agttacttta tagtttgaca
    40561 ttgttttagc agaaaaacct atttttgcta aatattaatg ttcaatcatg gttcagtcgc
    40621 taaacaataa atttgccccc aggggaaaca tctttttcaa atcaggtaaa ggcgggcatg
    40681 tgtatgccaa aatcgacaat cggtttgctg cctgtgaggt gtcgttgtac ggtgcgcagg
    40741 tgctttcttt tgagccgcat ggcgagcctg atattatttg gctaagcaaa aaaagccatt
    40801 acgataaagg aaaagccatc agaggtggaa tacctgtttg tttcccttgg tttggcccgc
    40861 atgccgagga aaaaacatat cctcagcatg gatttgccag gcttattgac tggcaagttg
    40921 aaaacgtcaa caacaatcct gacgggacca cttatatcgc cttatacctc tgtcaaaacg
    40981 attttacaaa aacttactgg ccttatgatt ttgaagcaag ggtgattatt actgtgggtg
    41041 agcaactgaa ggttgaactg aaggttacca ataccggaac tgaagaattt tcttatacag
    41101 atgcccttca tacttacttt catgtgagca acatagaaga catttgcgta gaaggattga
    41161 tgaacgctaa gttttataat ggccccggcg ctacacagct tgaggcccag aatgtagaac
    41221 aacttactat tactaaagaa gaaaaccgcc gatatattgg gcattctgga gcatgctcct
    41281 tcatcgacaa tggatacaac cgaaagatta ccattgaaaa aaccggcagc aacacaaccg
    41341 ttttgtggaa tccctgggcg gatacgatta aaactatggg cgacatgcct tctgatggct
    41401 ataaaaacat gctttgcatt gaacccgcca atgcttttaa tgatattatc gcccttaaag
    41461 ccggagagtc gcacacacta tcaacaatta ttggttattc aaaatattga gattggataa
    41521 acagttttta taaaaaccat tttaacatta tttgtcaaaa aacatgagac tgtgtaaaaa
    41581 gtaaatcacg cagcctctat ccaactgcta cgaccatcac caaaaattta aacagataat
    41641 gttgttagat aactttaatc aacaactgtg tttttaatat ttgcccaaga agttttcaaa
    41701 accatgtcct ttaagtttac ggcagtcacg agtatggcca gtaacaaatt gtgagctgcg
    41761 cctgtatctt acccccgtga ccaaccctga tgcggagtag aatgtcccaa actcgcactt
    41821 ccttcaccat tggctataca cgatgttggc agtatgaaac tctttatcaa gcatttaacc
    41881 tttcaatttc ttttttaata tcctcgataa cttctttatt tttaatctcc tttctttctc
    41941 caactttctc tgaaaaaaga taaaatactt tacctccaaa gacttcggta aatttcagat
    42001 gtgtgtcacc ttgaatgttg tattctttaa atatttcagg taactgtatc ccactattta
    42061 caggctttat ttgaagtcca atatatttct cttttacttg tataaaaaaa tcaacattga
    42121 ataatctgtc ccattcgtca ggtgctggtt gaattttaat gtttaatatt ttttgtaact
    42181 gaccataaat tgtttgtatt tctgtcatgt aaccatcaaa agttcggtca attacaagtt
    42241 gcatcatgta atcaatacaa tcttgttcag aaacttctgc aacttctgac tggattactt
    42301 cagtaatttt aacatacagt ttctttccta attcaacaat atgttctttg ggttttacat
    42361 ttttgaagta atattcacgc cattcatcaa gtgttttcgg cgaacatttt cttattgatt
    42421 ctgatgttgg tccaacattt cttttaaaat taagttggaa gcgattcatc gcactgttta
    42481 aaatccattc tttagccatt gttttgatgc aagaggttta aaaatttgtc tttgtattta
    42541 aagtcaatat cattgtccac ttgcactaaa ccatttttga ttaaatgagc gttaatgaaa
    42601 gttttgtttt caaggtagag atagcaaagc aagttatttt cgctgtcgtg ttttacaatg
    42661 tcgtatttca gaaagacacg tttccccttt gttttttcaa taagaaaaga agttgctttt
    42721 ccattaacta atgggtcttc tttaacgcca agaagtttaa tggtcaaatc gttacttaaa
    42781 cgaatttttt cagggctaat aatttctttt acagtaaaca tttcctctcg cttggttgag
    42841 ctttctttgt caattctgga accaaattga agtttcttta tgtcaatttt tttgtcaagc
    42901 gtatgagggt ctttgaaaat gtaaggcaat ttttgtattt ctttttcaaa gtcagttgta
    42961 atgggctttt gttgaataaa ttcataagtc gttccgttca aatctttttg atgaatttcc
    43021 aatttttctt taatgattgg aataaattca ggattgattt catagccaac cgaatttcta
    43081 tcggtatttt ttgctgccaa agaagttgtt ccgctccctg caaaagggtc aagaatagtt
    43141 tctccaacaa acgaaaacat tttgattagc cgtttgggta attcttcagg gaacatagct
    43201 atatgtccgt tctgccttgc tcctgcaaaa ttccaatgtc cggcaaaaaa cgtgttccat
    43261 tcttcagcag tcatttttga gagctctttc tgttctttag tgggtttagg gctatcgcct
    43321 aattttttga aaataaggat gaattcatag tccaatttaa gaataccgtt tcttgggtat
    43381 ggataagaac ccatttggac cccacctcca gttgtattgg aagttgttac tttttgccaa
    43441 atgatagctc ccatgtaatc aaacccaata ttttcacaaa actttatgat ttcttcacga
    43501 ataggaatga ctttataacg accataatac acagctcggg caaattggtc accaatatta
    43561 atacataatc tgcaaccgtt gtgaagagtg cgataacact ctttccaaac caaattcagg
    43621 ttattaatat aattttcata actgtcatga aatccaattt gattatcagt cccatagtct
    43681 ttcaattgcc agtatggagg agaagtaatt gccaaatgta cagaattatc aggcaattct
    43741 ttcatttgtc ggctatctcc atttattatt ttatgatttg ttttcactct ttattttttt
    43801 gacaaaagta caaaatctat cgtcaactaa ataattggtt gacaagttgc tgtttttgtg
    43861 ccattaccca caacattgca atagctatct attacagata ttctgtttta ctaaaattat
    43921 aaaaacggaa atatggagca acttgcgata agttttaata tttaatgctt ataaaagtta
    43981 tttttgcagc gtttttaaca aataaactta aaaaagatga aacaaaaaaa caaaatgaac
    44041 agggctgttg cgcaattcag gcaatggtcc aataagcgtt acgctgtatt caacagcttg
    44101 caccgcagta ttaaaatttg cacattggca ctggcttatt cgctggtatc aaaacccgtt
    44161 acggtaaaag cagaaagtgc agacactttg cagattaaaa accaggaaat tgacgatgtg
    44221 gtggtagtat cgtcgttgct cgaactgaaa aacttccaga caggccgctc ggtagagata
    44281 gtacagccta cccaaatcca gtcttcccct gcgacgtcga tagacgacct attacgctac
    44341 attccgggag ttgagtctca aacacgcggc gcatttggct cacaaaccga cttttccatc
    44401 aggggttcaa acttcaacca ggtactggta ctcatcgacg ggcaccccat cagcgagccg
    44461 ctcacagcac actacaacag caatcttcct atcacacctt cagaaataga ccatatagag
    44521 gtcatcaaag gccctgcatc gggcgaatat ggcgctgatg ccatgggagg agttatccac
    44581 tttatttcca aaacttttgc caaaaaccaa caagccaaaa accttcaggc cgagataaag
    44641 gccttgggag gccagcacgc ccttctgcaa accaatgccg gtggttattt tcagaccgac
    44701 agatggcgct ttgcagccgg aggctttatc aacaaatcgg atggaaataa gctttcctct
    44761 ggtttgtaca acttcttcga caaccgaaca tactcattct ctacacgata caacctgtct
    44821 gacaaatggt cgctggccta ccgcggagct attgacgaca gacattttaa tgcccaatac
    44881 tattactggg ccaacatgca agacagtgcc gaagaaacgt tgagcaggca acgccaccat
    44941 gcacagcttt gccgggcgtc agacgcttat actacaacca ttagtggcag ctacctgaat
    45001 acaaatgaca tttacaaaac caacagcata taccctaaaa atgaccaaac aacagaagtg
    45061 gcagcactaa aaatcaacca gcaatggact gtcaacgata aaataagact ggccgccgga
    45121 actgatgtcg atttacgaag gattgaaagc aacaaccgcg gaaaccatgg tacatacaaa
    45181 tatgccattt acctgcttgc ttcgataagg ccaacagaca agcttagcgt aaatacttct
    45241 gtgcgcaacg aaaagcacga gaaatatggc aatcaccttt tgccccaatt gagcctggcg
    45301 tacaagatta tcgacaacat aaatggcagg ctggcagctg cccgtgcaat ccgcatgcca
    45361 gactttactg aattgtacta taacaagctc aggcagaacg tgcctgttaa cagccgaatg
    45421 ggcaaccctg aactaaaagt tgaaaagtct tggaatactg aggttggcgt tgacttcttt
    45481 cttgaacgct tagccaatat tagtattaca ggtttttatc gcgaaaccaa gggactgata
    45541 gactatgtaa acatactttc ttctgaagtc ccattcttaa caaatctttt gcctaacacc
    45601 acttacaatg tagctttaaa caatagcact acaaaaacat ctggccttga aaccaatatt
    45661 tcaagaaagg tttcgttgaa cagcaaaagc tatatccaag tattggcagg ttacacatgg
    45721 ctacatgttg atgcttcatt tgaaaaaaat gcgttgccgt attacctagg caaccatgcc
    45781 aaacataacc tcaacggaac tgcaacaatc aggctaagta acctgcaaat agaggttaat
    45841 ggcttgtaca gaatccgaga tgccaaatat gaagccgcca tcaaccgcta tatggctgga
    45901 ggatatgcat tattcaacac ttctacctcg tatgcttttt acaaagaaag aatttcggcc
    45961 atcgtacaaa taaataacat cctcgaccgc aaatattctg acttcccggg cgttgatgcc
    46021 cccggacgtt ggatagcagc agggttaagg attaaattag agaattaaag aattaaagaa
    46081 ttaaagaatt agagaattag ctaatttgaa aatttggaaa tttgagaatt tgagaatgaa
    46141 tcaccttcga ttctgcaaat cgtaaacacg ataatttatt gtgggagcat actatcattc
    46201 cattaaaaaa taaatatgat gaaataaaaa tccctgttat tgctgtgctt tagcagggat
    46261 tttttataaa gtcggtttta acaactattg caactttatc ttttgactaa tgaaacgagc
    46321 atgcttagaa gtaaccacag acaaagatga ctcccgatta gtcggaacgg gtttttcttt
    46381 gaagggccct tcgaggttta tccaatcttt aaaattcaat tttgttcgga agataaaaca
    46441 caagtattat tggtagtaat atttttattt tctttaacaa caacaccaga gatataatca
    46501 tccttaatag taccagtaaa catctggctt ctccattagc caaattaggc aacacaaaac
    46561 gatcaaaaaa tagtccaaat aacagctttg gttttggtta ttatttttgg tatttttgtc
    46621 ctccctatta accttttaaa gtaaatgaaa ttaaacaacc tgctccaaag ccgcacgtcc
    46681 aaaataatac tgctcatagc tttagctttt atcatttttc ttgccataca aacaggaatc
    46741 tttcttaaac gcatatacag tgttaacatt gaaaaaaacg aaccgggcta cttgtatatc
    46801 cataccgggg caacattcac cgatgttttt aatgaattgg aacgcaacaa ttttttgaaa
    46861 gaccgcaatt cgtttatctg gcttgctggt aaaatgaact acgtaaatcg cataaagcct
    46921 ggaaggtata aaatcactcc gggcatgagc aacaaagacc ttatagtcaa gcttcgcaac
    46981 ggagaacaag aacctgtaga tattcccatc cacgatgtac gtacaatacc tcaactggca
    47041 ggtctgctcg acagatattt agagattgac tcaatccata tagttgaaaa attaagcaac
    47101 gaggcttatt tagacagtat tgggttcaat aagaacaacg ttttggcgat gttcatccct
    47161 aacacttatg aattatactg gaatgtaagc cttgacaaat ttgttcaacg catgttaaaa
    47221 gaatacaaca ggttttggac aactgcacgt aaagaaaaag caaaagagat aggtctttca
    47281 caagatgaag tatttacgtt ggcttcaata gttgaagaag aaaccaatgg tatatctgaa
    47341 tatccaatta ttgccggggt gtacataaac cgtttgaaac gtaatatgcc tcttcaggcc
    47401 gacccgacgg taaaatttgc catcggcaat tttgcagcaa aaagagtatt aaagaaagac
    47461 actggatttg attcgcctta taacacctat cgatataaag gcctacctcc cgggcctatt
    47521 tgtatgcctt ctatcaaggc tattgatggc gtgctcaatt atcagaaaca caagtacctt
    47581 tatttctgtg ccaaagaaga tttctcaggc tttcatgttt ttgccagcac gcttgtacag
    47641 cacaaccgaa atgccgaagc cttccaaaaa gcaatgaaca aaagaggaat ataccgataa
    47701 atcaaacaca acgacacaaa gacacaaaga aagtttttga attcaagaac gttgtgtcgt
    47761 tgtgtttaat ctagtatttt gccacaatag gcattctgcg gccaaaacca aaagacttgg
    47821 atgaaatacg caaaattggc ggagtctgaa aacgcttata ctcgttaaag ttaaccattt
    47881 ttatggtctt ttctaccaca gctttatcat aacccatatc aataatctct ttcttgcact
    47941 ttcggttttc gatatactga aataatatag catccagcaa tacatagtca ggcaatgaat
    48001 ctgaatcctt ttggtcggga cgaagttcag ctgaaggagc tttatctata atattttgag
    48061 gaataacatc tttatcttta ttcataaaat gagcaagttt gtacacatcg ctcttataaa
    48121 catcgccaat aacagacagt ccgccattca tatcgccata caaagtacca taaccaacag
    48181 ctatttcgct tttgtttgac gtattgagca atatatgccc aaacttatta gagatggcca
    48241 ttagcaaaac gccacgagta cgggcttgta tattttcttc ggtgatatct tcaggcaaat
    48301 tggcaaaaat aggactgaga cttgtcttaa agctatccac tacagattgt atattgataa
    48361 tatctttttg aacacccaaa ttttcggcca acttcaaagc atcgcccacc gaatgaccag
    48421 atgaatactt agatggcata agtaataccc ttacattctc agcccctaaa gctttaacag
    48481 caagcaccaa aactactgcc gaatcaattc ctccagaaag gcctaatgta gcttttttca
    48541 aaccactctt ttgaaaataa tcgcgaatac caagaacaag agcatcgtgc atcttttcaa
    48601 cttcaggttt ttcaatactt ctcttatctt caagtgtgat attcttagaa tcaataatcc
    48661 tgaaatcttc ctcaaacaag ttaagcgcac cagctacatg gcctttcgag tctatcacca
    48721 ttgagcctcc atcaaaaata aggtctgtat gtgcccctat ttggtttaca taaagtagtg
    48781 gcaacctgta gttcttagca tgattgcaaa atatctcttg ttttaacctg atttttgaat
    48841 atgaaaacgg cgaagcggca atattgacaa ccaatgaagg cctttgagct gccatttcgg
    48901 ccattggtga aacattatac atacgggcac gtttgtaagg tatgctttca tcgggaattt
    48961 catcccataa atcttcgcaa atagtaatag caatgcgttc gtccctaaac tcaattacct
    49021 taaactctga gcctggctcg aaataacgat attcgtcaaa aatatcataa gtaggaagta
    49081 aagccttatg atactgagca ataatctttt tatcatgcaa aaagaatgcc gtattgaaca
    49141 gccctttacc ctgagggtta gtattgacag acggtgcacc aacaatagca gcaattttac
    49201 cacaatgcaa ggctatttcc ttaacacatt ccaaagattt cttgacaaaa tactcatctt
    49261 cgagcaagtc gtgcggagga tagccacaaa cagaaagctc tgaaaagaca atcaaatcaa
    49321 cattttgttt ctcggccttt tcaatagcct caactatctt tttgcaattg tattcaaaat
    49381 ttccaatgat ataattctgt tgtgccagtg ctattaacat atacagtttt tttaaaacat
    49441 gaaaccaaga cgtatagcaa tacttctggt agttacttta tcttttgaat tatcagtaat
    49501 atctacaaag ccgcttgtat aagtaagtcc ggcaacaata gcatttccgc cacgcaacga
    49561 gtacattacc ccggctccaa tatgataccc caagttaaaa aaatggattt catctgtaat
    49621 attactatta tctaaaagtc cttttgcatc tccagaggta gcatgagcat taatattcat
    49681 aaatgatgtc aaaccaagat gtgcataaat agtggtatat ccgatttcgt gagatttaaa
    49741 cttcaggcca aaaggcacaa ccaagtattg aagattgtac ttaacgttag tattagcggg
    49801 taaattaact gctgaggtat catccttagt tgaaaacgag taagcataag tatttgatgc
    49861 actaccattt aacgaagcaa tagaaatacc cgtcgaaaaa gcataatttt cagcaaaata
    49921 tttatccata ttcaggccta catcgtaccc aaacgtagcc ccacctgttt tagtagattt
    49981 gacatcactt gcataccagt taatacaagg gttagcaaaa acagtaaatt tatacttcga
    50041 ttcctgcgct tgaaaaacct ggaccacgcc aagtagtata aaaaatatta cagctccttt
    50101 tgccatattt tcaggttttt aagttaatac acttttaatg ctgtaaaagt aaataaatac
    50161 aagttcagca aacccgttat tcattgaaag ttatcaacct tttatgaaac gagcatgttt
    50221 taaaatagcg tttcaacaaa gtttaaaatt atgcaataac agtgccattc ttaatgaaaa
    50281 tatgttgtat caataagaat attaggcaac tgaatacgca aatcttcggc atgttctttc
    50341 agtcgggcag cagcatcagg tggtaagtgc aaactatcgg aatggagctg gtaatattga
    50401 ataggtatta cataatactg atatcgggag gataacgttc ctttgtaaac ccagtatgga
    50461 atgtacatag gctttacatc aactttacca ctggttatct caatatttag tgttactaca
    50521 gcccctccgt ctttgtacct gtcacgctga ttggatataa agttgcccgt tgaataaaat
    50581 acaggcattt tattgaccga atccacagtt acccattcaa aagtttgtac aacatgggga
    50641 tgcgaaccaa caacagcatc aaagcccatt tgggcaagca tcctggcata cttcttttga
    50701 taatccgaag gagtaagctc atattcattg ccccaatgca caaaagctat tttaacttca
    50761 gctctctttt cttcagcaac cttaacatca gcctttattt gatccctgtc aagataattc
    50821 accacagaag gcgggtctgg cacaaatcca tttgtcccat aggtataatt gaaaagagct
    50881 atcttaattc catttttctc aacaatcaaa gggcatttgc tggctctatc ttctgcattg
    50941 tagtatgtac ccatccttaa aagctgtact gaatcaatca tctttaaagt tctgtacatt
    51001 ccggacttac ctttgtcata acaatgatta ttggctgtag ccaatacatc tataccaaaa
    51061 tctctaagct caaaaaccaa atcatcaggt gaagaaaatg taggataacc agtataaggt
    51121 tttccgccaa ataccacctc aaggttccca attgacaagt cacactttaa aactggttct
    51181 ttaataaact gaaaacacgg agcataatca taagtatcat ttgaagagtc atatgcagcc
    51241 ttaacttgag gcatatgccc cataatatcg ccaagaaaaa gtagcttaac atgacgcagt
    51301 aatgaatctt gcccaatcaa accataaggt tgacaacaaa gacagaaaaa taaagcagct
    51361 aagaaataac gttttaacat aaatgcaaaa tctcaaaata atagacaaag aaataatttc
    51421 tttagaacat attgatagag aagcattaat attttcaaaa aaaacatctc aacacttttg
    51481 acaaaacatt taaagaaaaa catgtttttt tcttaacttt aataaagaaa ttaacctgat
    51541 atgatccaga ctacccctaa taaagactta gaacaattgc tcaaaccaaa cctgtttgag
    51601 tcggtaaaca tactgatagc agaagatgac gaaaccaact attttctaat taaggaatat
    51661 cttgagtttt ctaatgccaa ccttatctgg gcacaggatg ggaaagaagc tattgatatt
    51721 ttaaaccaaa acaacaatat ccatttagta ctcatggaca ttcagatgcc agtcatgaat
    51781 ggatatgatg cattcaacct gataaaaaaa tcccatccaa agataccggt cattgcacta
    51841 tctgcttatg ctgttagtgg cgatagggaa aaagggcttc gccttggctt ctctgaatac
    51901 ctctccaaac cagtgtcgcg caaaaaactt gtagaaatta tccttcaaaa aattgaaggc
    51961 agttgctaac gtttcttttt aagcaaagca agcgtttcta catgatgcgt attcggaaac
    52021 atgtcgtaag gccataattc cttaacatca taaaattcgc caagaaactt caaatcccaa
    52081 gccagtgaaa cagggttaca acttaagtat atcactgttt ctggctcagc atacactatt
    52141 gctttcttta tctccatcaa agtaccagag cgcggcgggt ctaaaattac aacatcagct
    52201 tttccatgtt tatcaacaaa atcacgggta aaagtgtcga gaatatcacc taccataaac
    52261 ttaagcctat taaaaccatt ggcttctgca ctatatttag catcttcaat agcatattta
    52321 ttgccttcga tacccagcac agagcatcca tgctttgaca aaaaagaagg tattgtcccc
    52381 actccggtat aaaggtcata aacaaacttc ccaagagaaa cctcagctat ttgagcaatt
    52441 ttattgaaaa tattgactgc ttgcaacgga tttggctgaa aaaacgactg cgggcttatt
    52501 ctaaacctaa tctcatcaag ggtttcaaat atgtattttt ctccggccac ataaatatac
    52561 tgataatcct catatctggc atcattaccc ctaataacgc aacaataaac agaaacaaca
    52621 aacgaaaacc tctccattat ctgatttagc aaagtattaa tcatttctgt attgttttct
    52681 gaaacctgaa gaatcagcat ccatttatca tccaaatttt tacgcaatgt taccccccgc
    52741 aaaagtcctt gcttagactg ataatcataa acagaacaat ttatttcact ggctttatca
    52801 actataaact tgcacacctc aatcgatttg gagtccatca acaagcattc ttctacttta
    52861 aatacctgtt gtggatttcc cgaaggatga aaaccaaaca taatgttatt atcgccatta
    52921 acacctattt cattggcaaa agcaaagtct aatttattcc tgtaagcaaa aatattaggc
    52981 gaagcaattg ttttatgaag attaccagcc tttatctcat atttatcaag ggcattgata
    53041 agtatctgac gctttaaaac aagttgatgc agatattgaa tatggctcca attgcagcct
    53101 ccacactgaa caaaatgttt acatgcaggt ttttgtctat aacacgaagc atttaatatt
    53161 ttcgagattg aaccttctaa gaatgccttt ttctgacgtt tagcaatatt caggtttaca
    53221 aattcaccag gtatgccgcc ttctaaaaag acatttctgt ttttccattt ccctattgac
    53281 ttgcccgacg aatgaagccc aacaagctca acattctcaa catcatgaaa tttttgcata
    53341 atttgtaaaa tagccctgca aaaatgctat tttagcagca aaataaaaaa tttcaggnnn
    53401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg taaaatagcc ctgcaaaaat
    53521 gctattttag cagcaaaata aaaaatttca ggcgtcaccg aaatgctgac gcctgaaacc
    53581 caaccattat taaccttaaa aactatgaaa taaatacact tgtatctgta taaacgaaca
    53641 ggtagctaaa aagttactca cgtttgagta ttttaatcaa attttaatct tgctaagtag
    53701 ttgattttta ataaataaac aaatgctttc tatctgcatc cccacataca acttttcagt
    53761 ttacacactc gttcaaagcc tttcgcaaca agcagttaaa gagcatataa atactgaaat
    53821 cattgtcatt gatgattgtt cttcagacga attcaaaaaa aacaaccaaa acattaacaa
    53881 cttatctttg gtaaagtata ttgaactacc taaaaacatt ggccgttcaa aaatacgcaa
    53941 cctgttttta caatatgccc gttttgattg ccttctgttt atcgactgtg acgccaaagt
    54001 ctttcgcgat gattttatcc gccattatat tgaaaacatt gggaaagctg aaatcattgt
    54061 tggaggagtg gcttatgaca aagaaaagcc aaagaaatca atcaggctta gatgggaata
    54121 cgggcataaa agagaatata cacctttagg caacaggtta atgaagccat acgactcatt
    54181 caagaccttc aattttctga catctcgcaa cctgattgaa aatattgggt ttgaccaaag
    54241 tattagtaaa tatggacatg aagacacctt atttggtata gccttaaaaa atgcaggcgt
    54301 ttccatatta cacattgaca atccacttat tcatattgga cttgagccta ataatatttt
    54361 tcttagcaag acccaaaaag ctattgacgg gctttgggaa ttatattcct cccgaaagtt
    54421 tgataaaaat tcaaatattc gattattgaa cacctatctt aacttaaaca ttgctattag
    54481 caagtgttta gctttatctt ttcctgtttt ttcaacactt ttgaaaatca caatttggtt
    54541 tggtttgcct tccttggttt tattcgattt gtataagcta agttaccttt gtaaaatagc
    54601 caaataaaaa gtcttttata ttctaaaatc cggaaaagct tcaataaata aagataaaac
    54661 aataaaaaga gaaaaaacct gtcatcccaa caggcgttaa tttgacaata tcagaaaaaa
    54721 attctctgta caagggagat attaaatttt gaataaataa gaagcttcat aaaaattttt
    54781 atatttgcgg ttctatttta gaaaaattat aaactattaa ataccattac acatgcagaa
    54841 taaaggagct attaaattac tgaccatttt gcttacactg gtatgcttgt accagttgtc
    54901 ttttacactt gttacataca tatacgaaaa gaaagcaaaa aactatgccc aaggcgacct
    54961 caataaagaa actatgtatt tagattcaat tgcgggcaaa gaagtatata attttttgta
    55021 tttaaaaaaa ttcacttacc gtgattgcaa agagcgtgag attaaccttg ggctcgacct
    55081 taaaggtggg atgaacgtgg ttcttgaaat ttcagttgca gacatcgtta atgccttgtc
    55141 aaactatagc caggacagta cttacctcaa agccatgtct attgcacgag aaaatttgaa
    55201 aaccagccaa aaagacttta ttacattatt tggcgaagca tttaaacaag ttgaccctaa
    55261 tgcccgcatg gcagctattt ttggcacgcg cgagctgaaa gataaaatca actataacac
    55321 cagtaatgaa gaagtactta aagtattgag agaaaaatca aatggcgcta ttgacaactc
    55381 tttcaaaatt cttcgcaccc gtatcgacaa atttggggtt gtatctccaa atatccagaa
    55441 aatggaaact gcaggccgtt tgcttgtaga acttcctggt gttaaagacc caaaacgtgt
    55501 acgcaagctc ttacaagggg ctgcaaactt agagttttgg gaaacatacg aaaatagtga
    55561 aatatacaat tctttattag aagccaataa acgaattaaa gctttagaag agtcaggcgc
    55621 tgttgataca acaaaaacag ccactaatgc tacaaacaat tcagcaaaag ctaataaaaa
    55681 tgttgctaaa aaagattcaa caaaagcaga agtggcattg cttgaaaaat tacaaaaaga
    55741 tactacaaaa actgattcta tatcaacacc atcacaattt aacaaagagt atcccttatt
    55801 tgcattatta cgtccggcta tcacacgaga caaccagctt atgccaggtt cagtagttgg
    55861 atatgccagt catattgaca ctgctaaaat taacttttac cttaaccaga aacaagttcg
    55921 cagtgctttt ccacgtgatg taaaattttt atgggctgta aaagcagtta aatacgaccc
    55981 atcagggtca acttttgagc ttcatgctat taaagttacc agccgcgacg gccgtgcacc
    56041 gcttaccgga gatgttgtag taagtgcacg ctcagacttt aaccaaaaac aggctacagc
    56101 ttctgaagtt tctatgagca tgaacggtga aggaacaaaa atatgggccc gtttgacacg
    56161 cgacaatatt ggacgttgta tagctattgt tcttgacaac gctgtatatt cggcccctcg
    56221 ggtaaacagc gaaattaacg gcgggaactc tcaaatcaca ggcgacttca caaaagaaga
    56281 agccgacgac ttagctaacg tgctcgagtc tggtaaacta cctgccccgg cacatattat
    56341 tcaggaaact attgtaggcc ctacccttgg taaagagtct attatatccg gtttgctatc
    56401 attccttgtt gcttttatag cagtattgct ctatatgtgg ctatattata gcagagcagg
    56461 tgctgttgca aacgttgcat tgtttgcaaa cgttgtgttc cttttaggca ctatcacctc
    56521 attaggtaca gtattaactt tgcctggtat tgccggtatg gtacttactc tggctatggc
    56581 agttgacggt aacattatta tttatgagcg tatccgtgaa gaagtaaggg ctggtaaagg
    56641 tataaggctt gccgttaaag atggattctg gcatgcatat tcagctatta ttgacggcca
    56701 cgttaccaca attttgacag gtattgtgct tttcatcttt ggctcaggcc caatccaggg
    56761 ttttgctgta acccttatca ttggtttatt actttcattg ttttcttcaa tattcattgc
    56821 ccgtttgatg tttgaatgga tgatggacaa agaattccac atcagtattg gaaataaata
    56881 caccatggaa atctttaaac atattaatat agactttatc gggcttcgta agaaaatgta
    56941 cattatctct tctatcttaa ttgggattgg tatagtatcg ttatttataa gaggtttaga
    57001 ccctagtgtt gagtttaccg gtggccgtac ttatatagta aggtttgaga aacctgtaaa
    57061 aactacagaa attcgtaata tgttgacccc aaccatgcaa tctgcccctg aggtgaaaac
    57121 atttggagaa gacaaccagg taaaaattac caccaaatat ttgattgatg ataaatctgc
    57181 aatagcagat tcgttagtag aagtagccct tttcaaaggc ttgtcttcaa aacttccttc
    57241 aggtatggac tttacccaat ttaccgaaca gcaacaaggc aaagcatacg gtttgatgag
    57301 tgcgcaaaaa gttgaccctg ttatgtcaaa agacttggta tatcaagcat tccttgctgt
    57361 aatatttggt ttgattatca tctttattta tatagcgata agatttaaaa actggaagta
    57421 cggactaggg ggcgttattg ctttgtttca tgataccatg attatcattg ggttattctc
    57481 attgctgtac aaatttttac cgttcagcct tgaagttgac caacaattta ttgctgcgtt
    57541 gcttactatc attggatatt caattatgga cacggttatt atttttgacc gaatccgtga
    57601 atacaacaaa ctgtatccaa aacgcgcttt ggccgaaaat atcaacggag caatcaacac
    57661 tacactggga cgaacaatta atacttcagg tactaccata gtaacattgc ttgtaatatt
    57721 tatctttggc ggcgaaatgc tccaaggctt tgtatttgcg cttcttatcg gggttatagc
    57781 tggtacatat tcatctgtat caattgcaac accaatagct tacgatttga tcaacatgat
    57841 aagcaaaaaa aagaaagcat aattctttaa ataatttata aaaaagcggg atgtcaaata
    57901 tgtcccgctt ttttatttta caacttatcg ctaattttga acttacttga aatagatggc
    57961 tgatggataa ttttagtaaa atcacagacg attcacaaaa cagagaatac tccaatgacg
    58021 aaataactgt attttggaag cctggaaaat gcgtacatgc tacaacctgc tataaagaac
    58081 taattgaggt attcaatcca cggcgtcgcc cttgggtcaa tatgtacgga gcccctaccg
    58141 aacgcattat agaaatagta aataaatgtc ctacggaagc actcacttac aaatggaatg
    58201 acgaggctaa aaacaaggaa accataaacc tcaaaaacga aaggctaaaa aagggcgagg
    58261 gacaaaatgt taccagccaa caagctaagc ctgttaccat acaagttatg cgtgatgggc
    58321 cattggtcgt tcaaggagac tttgaggttt ttgacgaaaa aggcaataaa cttaaacaca
    58381 tgattatgac ctcgttttgc aggtgcgggg catcaaacag ccagcctttt tgcgatggtt
    58441 accatagaaa aattggtttt aactctgaaa aataaaccgt aatggaaatt tcaaataaca
    58501 atatccccga attcaaagtt atagctaatg gcccgctaca tgtaaagggc aaaataacgc
    58561 ttatcaacac taatggcgaa aagcaagagc ttgaagaaac atggctttgc cgttgtggca
    58621 actcgaaaaa caaaccattc tgtgatggtt cgcacaaaac tgccggattt tagcattgag
    58681 ccgtaacagc ttttttcacc agttctgcag cttcttccaa ggttgtggct gtcataactt
    58741 tcaatccgga agatactaat aaatctctgc cctcttttgc atttgttcct tgcaaccgaa
    58801 ctataattgg tatttttatt tcacccacat ttttaaaagc atcagcaatg ccctgagcaa
    58861 ctctgtcgca acgaacaata cccccaaata tattgaccag tatagctttg acgtttttat
    58921 ctttcaggat aatcctaaac ccggcctcta cagttttagc actggcagca cctccaacat
    58981 caaggaaatt agccggatta cctcctgaaa gctttattat atccatagta gccatggcta
    59041 accctgctcc attaaccata cacccaacat ttccatcgag cttaacaaaa ttaagcccat
    59101 gcaaagcagc ctctgcctca gcagggtctt cttcatcaat atccctaagt cctaccaaag
    59161 atggctgtcg gtatagagcg ttatcgtcta tcacaacttt agcatcagct accagaagct
    59221 tgtcatcaga tgtttttacg agagggttaa cttctatcat acttgcatca agcttttcaa
    59281 aagccaaaaa catattaccc agcaaaagca aaaactcatt gactaatttg cccgtcaaac
    59341 caatattata ggcaattttg cgagactgat aatcgtgaag cccaatcttt gtatctatta
    59401 attcggtata aataacatca gcattggaag ctgcaacagt ctctatatcc atcccgcctt
    59461 tggccgaata aacaatggca tacatgccct tgtcgcggtt aagcaaaatg ctcagataga
    59521 actctttgat ttccgaaact ccagtataat aaacactttg ctcgacaagg acttttctga
    59581 ccagcttacc tttagggcct gtttgtggtg taaccaacgt cattcccagc atatcatgag
    59641 cataatgctt tacttcttca agattatgag ctaccttaac acctccgctt tttcccctgc
    59701 ccccggcatg tacctgggct ttaacagccc atgcattcgt accagtttta tcagctatct
    59761 tctttgctac ctcaatggca gcaccagccg attgggcaac aaaaccttcc ggtacaggaa
    59821 ccccgtattg tttcaaaagc tgttttgact gatattcatg taagttcatg atgaccaatt
    59881 actgatgact aatgactgat taaaacaata tcattcgctt tattcagata aattcatgca
    59941 gatgaaaggt tattatagat tctgcttttt ataaagataa aactttttta ttttcgcaac
    60001 tacaatgatt aaccaaaacc aatgcgaaaa ctactcaaca gcgaactgaa aagacttacc
    60061 cccgatgaat ttaaaaaagc cgacaaaata cctgttatca ttgtactcga taatattagg
    60121 agccaacaca acgttggcgc tgtattccgc acatctgacg catttttgat tgaggctata
    60181 tgcttgtgcg gtattacggc tacacctcct aacgccgaga tacataaagc tgctttaggt
    60241 gctgaagata ctgttgactg gaaatactac aaaacaacat ctgaggctat ttcagaacta
    60301 aaagaggcgg gttattctat tattactgtt gaacaagctg aaaattcaac tcctctggag
    60361 aaatttcatg cccactctac aaaaaaatat gccatcgttt ttggtaacga agtaaaaggc
    60421 gttgaccagg agattgttga cataagcgac aaatgcattg agcttgccca gtatgggaca
    60481 aaacattcta tcaatgtttc ggtcattgca ggcatagtaa tttgggaact tttcaaacaa
    60541 ctaaagctgg aaagataaag gcaactcaat caaactccat attttactta acctccccaa
    60601 aatgaaaaaa aaaattcttg catttttact gctatcactc tgttcacaac tgatttttgg
    60661 ccaacgctat accattagcg gcaccataac tgatgcttca aatggcgagc tactgcccta
    60721 ttctactatt attgactctg tttcaaaaaa aggaacttta gccaattatt atggctttta
    60781 cagcataaaa ctcgataagg ggcctgtaaa aattatgtgt agctacgcag ggttcaaccc
    60841 aagcataatt tcttttaacc taaccaaaga caccaccatt aaccttacta taaactcaac
    60901 tacagagata gaagaaattg tagtcacctc gtcaactaac aaactcagga gtactcaaat
    60961 gggacttgta gaaataccaa tacaagaaac caaacaactt cccgttttgc ttggcgaagc
    61021 tgacctgatg aaaaccatac agttgatgcc gggcatcaaa gggggaactg aaggcaccag
    61081 tggtatatat gtcagaggag gtgggcctga ccaaaacctg attttgctcg atggtgttcc
    61141 ggtctataat gccagtcatg cctttggcgt tttctcggtc ttcaattcag atgccatcca
    61201 gagttttaac ctgatgaaag gtgggttccc ggcccgttat ggaggtcgcc tctcttctgt
    61261 tattgatatt aaaatgaaag aaggaaataa caaagaattt cacggcgagg gctctatcgg
    61321 cattatatca tcaaaactaa cactcgaagg cccaataaag aaagaacaaa gctcatttct
    61381 catatctgcc agaaggacat attttgattt atttacagta ccaataacta tggctgcttc
    61441 agacggaaaa aatgttggag ggtattattt ttatgatgtt aatgccaagg tgaaccacaa
    61501 attttctgaa aaaagccgat tgtttctttc cttttacaca ggattagaca aggtatatat
    61561 gcgagaaaag agcagttatg aaggcaatac cagaaaaaat gaggctggta caggttgggg
    61621 caacatcacc actgccctga ggtggaacta cattttgtcg gacaacctgt ttgccaactt
    61681 tacaggtaca ttctcgcagt ataaattttt cattgaagaa gaatcgaata actccaataa
    61741 taatgatttt ttcagctatc aatacaattc gggcatagtt gattatgcag ctaaaataga
    61801 ttttgattac atcccgcacg ctgcccacaa tattaaattt gggacaaact caattataca
    61861 tacatttaac ccgggcgtaa acgtgcagaa agaaaatgga gggaacaata acattgacac
    61921 tacttttggc aataaaaaca tctatgctac tgaaatttcc tcatatattg aagatgatat
    61981 agaatttagc ccaaagttta aagccaacat cggactgcat tattcttttt tctctgtccg
    62041 cagcagaaca tacaactcat ttcagccacg tctgtcgggc aggtacctgc taaataacaa
    62101 tattgcacta aaagcctcgt atatacaaat gatgcaatat atacacctgc ttaccaacag
    62161 tactattggg cttcctacag acttatgggt tccctcaaca cccaatatca agccacaaga
    62221 agcatggcag acagccttag gaaccgaaat atctctcaat aaaggctatt ctttgtctgt
    62281 tgaaggatac tacaaagaaa tgaaaaacct tattgaatat aaggaaggtg cttcattctt
    62341 ttcagaaaaa gacgactggg agggaaaagt agtacaagga aaaggctgga gctatggcgc
    62401 tgagtttctc ttatcaaaaa gtactggaaa aataacgggc tggataggat ataccttatc
    62461 ttggacaaac aggcaatttg aatcgataag ctttaacaaa gtttttccat acaaatacga
    62521 cagaaggcac gacattgcca tagctctcac ttacaaacat tccgagacct ttgacatggg
    62581 agctacttgg gtttacggca ccgggactgc aacaacctta gctaccgaag aattcaacgg
    62641 ctatgatacc ttctttgaca atttcaacat tccaaaatta tcgggagcct atgtgcacta
    62701 ttatgaaaac aaaaaccaat actttgaaca ccgaaacaat tacagaatgc ctgcttatca
    62761 caggttagac attgggttta attttcacaa acaaaaaaaa tggtataaac gcacctggag
    62821 cattggcgca tacaacgctt ataacagaca aaacccattc atgcttgtgt tggaaaaaga
    62881 ctatgagagt ggtcaaaaca aactttatca atatagcata tttcaaatac tcccctattt
    62941 cagatggtct ataaaatttt aaccatgaga actaaacatt tagccttttc tattttattt
    63001 gtttttatag cccttattat tgggggctgt agaaaggaga tacctctcaa cattgacagt
    63061 aaagacagaa aaattgtggt aaacggcata cttattccag acagcgctgt ttgcattaac
    63121 ctgtctaaaa gctcgtctat tgccgaaaaa gacctaaacg aatataggta tattgaaaat
    63181 gccaatgtgg gcttatttga aaatgggata cttgtaaccc atttaacttc aacgaataat
    63241 ggatattaca aatctgacaa cgactttagg cctaaagctg gcaactttta taaactaatt
    63301 gttgaacatg aaggaaaaac agcctcttcg gagttctttt tgcctaaaaa agagttaaac
    63361 catagcattg acgttaaaaa aatacgaagc tatagttttg aaaaagacga ttctgtagta
    63421 tatagtctaa aaatttatat cagcatcaac gacaatggaa ataacaaaaa ttattattgc
    63481 atttcgttcg ggtacccggt ttttcaaaat agatactatg gaaaagactt accaataagt
    63541 caaataataa tctacaattc taaatttaca ttatacagcc cagcggtaga attctcaaat
    63601 ttcaattact taaacgatga tgaaaaatta gaaaaaggca tttatggtca atttgtttat
    63661 ttttccaatg aactattcag ggggcaaaaa cataaccttc gccttgaaac aaaccttagc
    63721 cctttttatg taaaagaagg aaaagatagt gccacttttt acctctatat ctcgatgatt
    63781 gatgaaaact atttccgcta cagaaagtat atagcagaat acgataacga tgatgatttt
    63841 ttttcagaga aagtccagat agtaaacaac ataaacaatg gaattggcct attttcaggc
    63901 ataacctgca ttgtggattc attttcaatt gcaataaatg aatttccctc catgcttatt
    63961 cagcaataaa tgaaacgagc atgtttagaa gtaaacacaa acacgtatga acaaaatcct
    64021 gcgagtccca tccgaccttt aaaattcaat tttgttctca tgaaacatta aaccaatacc
    64081 acttatttcc aataacaaga aagctaacac tttatctatt ttcaaatttt ttaatccaaa
    64141 aaatccgata tttgcgttct taaaaaatga acattgggta gaattttagc gttagattat
    64201 ggaacaaaac gtaccggtgt agctgtcacc gatgcgttgc aaataacggt aaacagtctt
    64261 ggcactgttg ctaccaaaga tgtttggagt tttttggaaa cgtattttgc caaagaagtt
    64321 gtagattgcg tagtgatagg ctatccactg caaatgtccg gtaaaggctc tgattcgtta
    64381 agggtgataa acccttttat acagggcttc ataaaaaaat acccaaacat ccgcattgtt
    64441 caggaggacg agcgtttcac ctccaaaatt gcccaacagg caatacttgc aggtggcgtt
    64501 aaaaagaaag acagacagga caaagctttg gtcgatacag ttagcgcagc cataatactg
    64561 caaagctatc tggacaggct tagattgacg aacagataaa atttgacaat tatgatactt
    64621 cctatttatg tttacggaca accggtactt cgaaaagtag cagaaaacgt cacaaaagac
    64681 tatcccaacc tggaaaagat aatagccgac atgtgggaaa ccatggaaaa atctgatggt
    64741 gtaggccttg cagccccaca aattggcaaa tcaatacgtt tgtttgttat tgatgcctcg
    64801 cattttgccg aagatgaccc aaaaacagaa ggtttccgta aaacatttat caacgctcaa
    64861 attctttcca gagaagggga agaagtgcta ttcaacgaag ggtgccttag cgtacctggc
    64921 attcgtgaag acataaagag gccttccatt gttaccatac gttacatgga cgaaaacttt
    64981 gtagagcatg aagaaacata cgatggtata agagcccgtg ttgtacagca cgaatatgac
    65041 cacattgaag gaaaactcat gatagaccac ctcaatccgt tgaagaaaac attgctcaag
    65101 cgtaagctaa acgacatcag caaaggtatt gttgatgtaa attataaaat cagggcggtg
    65161 aaataaacaa ttacaaggtc ttccaggttt tagaaacctc gaaagtttgt cttataaaag
    65221 ccttctataa ttacaatatt acttcaccag ctccctagcg ggttacaact agctcatcgc
    65281 ctgtacaatc aatgatggta gaaagtaccg ttcctcctat accgccatca ataaccaaat
    65341 cgacaagttt ctcatacttc tcatgaataa gctctggatt ggtaatctcc tcgtataagt
    65401 cgttatctga atgtacagat gtagtaagca ccggattgcc taaagccttt acaatttctc
    65461 taataatatt attattaggg atacgaatcc ctacagtcct tttcttttcg agaaagaaac
    65521 gaggaacatt gctggaagca ttgagaataa aagtatatgg gccaggcagc aaacgcttca
    65581 tcaacttaaa agttgcattg tccacttgcc tggcataatc agacaaatgg ctaaagtcgt
    65641 agcagataaa agcaaagttt gctttttccg ttttggtttg tttgatgcgg gctatacgct
    65701 ctaccgctgc tttgttgcag atatcacaac caataccata caccgtatct gtgggataga
    65761 tgattacacc gcctttacgc aatatgtctg ccacagcttc cacatcgcgc tcattaggat
    65821 tttcgttgta aaggcgctta atcatatttt ttttgtaaat ataacttctt tgaacaatta
    65881 cttctctatt tatattccat aaaacaagca ggcaaaaaaa agatttatat ttttgcacaa
    65941 acaaatttca aattatggat ttaagagaga tattatctat ttcaggattt ggaggacttt
    66001 tcaagctaat ctcacaaagt cgcaacggat tggtagtaga aggcctggaa gacaaaaaac
    66061 gcatgaacgc tttttcacac tacaaggtta gttcactgca tgatatagct atttataccg
    66121 acaccgaaga aattcccctg cgaaaagtgt tggctaaaat ctatgaaaaa gaaaatggac
    66181 aacaggcttt tggtggcaaa attgatgaaa aacagcttaa agcttatttt gaaaccatct
    66241 tacctgacta tgacagagat agggtttatg tttcggacat caagaaagtg atttcatggt
    66301 acaatattct tgttcgctgt gggtttactt catttgaaga tgcacaagaa gctaaaaaag
    66361 atgaaaccga aaaagcaaca aaagaataat atttaattta ttgataacaa caaaacagcc
    66421 cgggcaaaaa atttccgggc tgtttttatt tttaaataaa ttaagccttt caacatataa
    66481 acccatatta gcaataaaaa tgcttaatcg attaagaaat tgtaattcaa taacttaata
    66541 aaatttttca aacaatttct aaatcagaat tgaacccagc taaaccatat agttcggcaa
    66601 aaaacctcct aatgattaat ttgggataaa tagtaaaagt ggcaaaattg agcttttaaa
    66661 agctctctta tgttgcataa aaaattacct ccaacaattc ttttattgaa tatgcacttt
    66721 taacaactat ggtttttatt tattttgtta tatttgataa aagcataaat cagcctctaa
    66781 attttttaaa ggaacaaatg gaatgcctac attttattca tcactttggg tgagtataga
    66841 atcatggaca tttcatcata acaaaattat ttttaaggac tgacagcatg ttctttattg
    66901 ggtggggaaa tacccatgta attgattttg ttatcattgt gtttatgttt gcaaaatatg
    66961 gttatttcat ccaaacaaaa ttgatttttg atggttaaat agcttgaact gtcttaggcg
    67021 gaagaacact tttgaaaaca attctatttg taagtatttg tgtattaata cataatatat
    67081 ttaatggaat gataacttgt agcatagttg acgggagaac accaaaaata aacaatatcg
    67141 tgtttattca cttcgtccat caaagcgctt tgtatagttg ctatgggcta ccataggtat
    67201 tttaaggaaa agagcttttt ttggggggag gtatctgtac aagctgtcag gctgataagg
    67261 aacaataata aagaaataaa ttgcaaaaaa cttaacctac ttgaccaatg gaccttgatt
    67321 tttaaataga aaatagcttt aaaatttcag agctgataac aaaaaagtac agcacctcgt
    67381 tttcgttggc tacttcactg ctggaaaaag aaaaacgaaa agcgatatat gccatttacg
    67441 gttttgtaag gttggccgat gaaattgtgg acagctttca cggtttcgac aagccttttt
    67501 tgctcaacaa cctggaagaa caactgcatt acgccttaca aaacggaatt agcgccaacc
    67561 ccatcttggc agcttttgcc aggactgtaa agaaatacca catcgacaat gaccacattc
    67621 aggctttcat gcaaagcatg aaatacgacc tgaccaaaac tgattatacc agcaatgaag
    67681 agctgaacag ctacatttat ggctcagcca atgtggttgg gctcatgtgc ctgaaagttt
    67741 tttgcaatgg cgaccaaaca cattacgaca aacttgaatt ttcggcacaa aagcttggag
    67801 cagcttttca aaaagtaaac tttctgcgtg acctggataa agataaaaaa gagctcggac
    67861 gcaattattt ccccgaaata tgggacaata ctttgaaaat agacaataaa caacgcattg
    67921 aacaatccat agaagacgat ttcaacgagg cctggaaagg tatcaaacaa cttccgggcc
    67981 gggcaaaact tgctgttgcc atagcctatt tttattacag ggcactgttt ggaaaaataa
    68041 aacaaacaac gcctgaaaga cttatggcag aaagggtacg ggtaaacaac ctgaataaat
    68101 accttatttt actgaaggtt ggaatgatgt acaaaaccag gcttttttaa aaatcggcaa
    68161 aattctaacc atgaatgtta ttttatcata gatgaatctc tacccgcgga atatattgcc
    68221 acaaaaggaa gtaaaaaaat ttgttgtaat tttctacact gtaggcctgt tggggtttat
    68281 cattcctttt tcgcgcgatt tcttcataaa aataacccct ttagccttat taatcagttc
    68341 atacctgcta tttacctatc ataacaatat atcgaaaaaa gatgtcgcag catttatgct
    68401 ggtttttgtc cttgggtttc tgatagaagc agcaggggta aacactggcc agatatttgg
    68461 ccattacagc tatggcagta cattgggccc tgcattatgg ggcacaccgc tactaatagg
    68521 cttaaactgg ttgtttttgg cttactccag cctggtagtt gccgaaaaaa ttagccataa
    68581 agccatattg caaatcgcca ttgccccaac attaatgctc atctatgatt tggtgctgga
    68641 acaagtagcg cccatattgg atatgtggac ctggcaagat gcatcggtac cgctgaagaa
    68701 ttatatagcc tggtggtgta ttggactggc ttttaccggc tttttaaaaa tatcaaaaac
    68761 agacaccagc aaccccctgg cgcttatcct tttggtttgc cagtttgcgt ttttcttgct
    68821 gttattcctg gtttttaact tacttctatg attaaagccc ggcaccatat tgtcgtttac
    68881 cctttattca aatggcttac ccgctttctg ataaaacgca atttcagggc tgtgcatgta
    68941 gaaggctact ttactgacaa ccacaaagca atactgacaa tagccaacca cgtaagctgg
    69001 tgggacggtt tttgggccga atacctgaac caaaaaatcg cccagcgcag attttacttt
    69061 atgatgctgg aacaacaact gaaaaagcat tggtatttca gctacacagg aggttactca
    69121 ataaaaacaa agtcgcgcga agtagttaaa agcattgact acacggccga attgcttcat
    69181 aaccaggaaa atatcgtcct tatgtttccg caaggcaaga tacattcggc atacaacaac
    69241 gacattcatt ttgagaaagg tgttcaacat attgtaaata aaagccccga cgatatgcag
    69301 gtattgtttg tagccaattt taccgactat ttttcagatg ccaaacccca cttgtatatt
    69361 taccatgagg cttatcctgc actatttttg aaaaaaaatg atattgaaaa ggagtataac
    69421 cagttttaca cacgcgtgct gaataagcat aaaatcaagg cttcctgatg ctatacttat
    69481 tgtacatatc atcggccttc attgctttgc aactgttcaa cgtgttgctc aattttttat
    69541 tccgtcaaaa gattgaaaac actgacaggc agaactcctg catgctgtcg gtgcttatcc
    69601 ctgcccgcaa cgaagaagcc aacatagggc aacttctcga aaagttgcac aacatgaaaa
    69661 acgagcacgt tgaaattatc gtgtgcgatg accattcgga ggacaaaact attcagatag
    69721 cccgcgaata ttgtaaaaaa gacccccgga tacagttaat gctttctgat acgttaccta
    69781 caggctggtt aggcaaaaat cacgcctgtt ttcagctggc gcagaaagca cgtggcaact
    69841 acttcctttt tgttgatgca gatgttaggc tgcatggcaa tatcctggcc gatgcagttt
    69901 cgtacatgaa aaaacaccgg ttggggctgc tttcggtatt tcccaaacaa atacaacaga
    69961 ccataggcga aaaagcgagt gtccccatca tgaactatat tttattgacc ctgctgccat
    70021 taatatttgt aagggtttcg ccatttaagt cgcacgcagc agccaacggg cagtttatgc
    70081 tgtttgatgc cgaaacgtac aaaaagtttc agcctcatga acaattcaaa aagtctgcgg
    70141 ttgaagatat tgaaatagcc aggtttttca aacatcataa aataaaaacc gcatgtctta
    70201 ctggcgaaac acgtatagag tgccgaatgt acacctccta tcaggaagcg ttgaatggtt
    70261 tttcaaaaaa tatctttatg ttttttggca atatccctgt gttggctttc attttctgga
    70321 catgtgccgc cttaggattt ttgccatttg taatgcccgg gcagcaactg cttgcagaat
    70381 accttattgc aatagtgctg atacaggtgc tttatgctgc aagttgcaaa caacctgttt
    70441 tttacaccct gctgcttttc ccggttcacc ttgcttttat gttagagatg atggtaaaag
    70501 cgctgatagt taaatacaac aaaaaatacg tatggaaagg aagaaatata tacaaattgc
    70561 attcataagt ttattgcttt acccgctatc agggcaaaac aaccatacca tttatcatgc
    70621 ttttgctgca ggaaacatga aaaagtggaa aacagccatg gactcgattg aggctataac
    70681 acacaaaagc aacaaagagc tgctggattt gctcaattat caatatggct acattgcctt
    70741 taacctgggc aaaaaaaacg cccaggaagc agacaaatac atgataaagg catacaccga
    70801 ccttaaactt ctggaaaaga acaaatacaa cctgccagtg ttatatgcat ataaggcagc
    70861 gctaacaggc tttgagattg gcttacaccc ctacaaagcc ccttttattg gcccaaaaag
    70921 cattgaatat gctaagatat ccgtaaaact tgactctgcc aatgcaatgg gatatataca
    70981 gctgggcaat attgcctatt acatgccttc tatgtttggc ggttcaaagg ctgaagcttt
    71041 aatctattat cttaaagcat tgtctatcag tgaaaagaaa atagagtgca aaaaccacaa
    71101 ctggaactat ttgaaccttt taagtatcat catcaaggcc cacatggact taggacaata
    71161 tcaccatgct gaaacatatt gcaaaaaggc gttggctgtt gaacccggtt tcgattgggt
    71221 caaaaacaac ctttatcccc aagttttaaa a
//
LOCUS       DAOSPF010000009        68595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_430_length_68595_cov_7.148468, whole genome shotgun sequence.
ACCESSION   DAOSPF010000009 DAOSPF010000000
VERSION     DAOSPF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 68595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_68595_cov_7.148468"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1790
                     /locus_tag="PK252_03130"
     CDS             156..1790
                     /locus_tag="PK252_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13734.1"
                     /translation="MKKIDKYILPICYTIVFIIVIMMFNALEFNSNATYIGFNVLTNS
                     DSCLLVQISKDILSDPKAIFDWHYGTCFYFFPNLLIVLLLTTFLSTPSIIIFVNSLLI
                     FFITLFLLNYLFLRILHISKICVAIANLSLTLFLAFTADSSDFCFLTPHLFMPLHSGA
                     LINALGSLILCFMYLNNNNKTSLVFLIILSIVAMFSDFIFLIYFSIPLVLTLLTLYFF
                     GKKQINRKKYITVVLLLVFSYIFTFILHKISINSGIFMLEAFKNLFYNIKDSFILMFN
                     SIFEVFTKNYISIIIFFLSSFSLVLSFFHALKYLFFNKNKKNIISENSLLYSIFTCYF
                     LVLIFAATIINGSFQGIDCIRYIIPPFYIGIFNIGLYIHLYAHKRNKVGLTRIIALSF
                     ISVYTFFIILNYSLINPSSLYLKVKNYYPPMIKAADNMSLKYKVKNGIGNYWDARFIT
                     IFSKQGITVDLVSPEYTPMKFANNPRKYYTNYNNDSVYYNFIVNYSNEDTAKLYQVFN
                     KKNITKDTIEGNIFYLLPSFYLENDFRTFRIKEINN"
     gene            complement(1780..2526)
                     /locus_tag="PK252_03135"
     CDS             complement(1780..2526)
                     /locus_tag="PK252_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT13735.1"
                     /translation="MIKNKIVTVVLPAYNASKTIESTYNEIPFDIVDNVILVDDCSKD
                     NTVEVARKLGIKEIVVHDKNKGYGGNQKSCYKRALELNSDIVIMLHPDYQYTPKLIHS
                     MSYLIANDVYEVVLGSRILGRGAIKGGMPLYKYFFNRFLTFTQNKLMHQKLSEYHTGY
                     RAFSKNVLTKINLEANSDDFVFDNQMLAQIFYAGFEIAEITCPTKYFDDASSINFRRS
                     SKYGLGVLKVSLMYFLQKIKVGKFKIFANC"
     gene            2930..6043
                     /locus_tag="PK252_03140"
     CDS             2930..6043
                     /locus_tag="PK252_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT13736.1"
                     /translation="MYPLLLLIFIFVVIISMLVYVIIQKMLISKLSNELKNRDNLNKL
                     ITENINEAVCTINSEGIIQHASQSYVSVIGFAPSEITGKAIYSFIHEKDKENVKQLFE
                     TTLHSSTEKVFDFRHLKPDGSYIIVEAYARLLSSSEIQHEGIILSMRNVSELRISELQ
                     YYFSRFALDNVQQSVVMIDPSGSVVYLNEKTRQILKKRHISAQNLKIWDISPDVEKEN
                     WPEYFEHMRRNKYRVISSEIKTPVGNIFLERHATYTEFKGKEYIVTNSIDLTRYFKSE
                     ELIRASEQRYKRIIDNIYDVVWVLDMELKTTFITPSIERLTGYTVEEHMTQSIEEKFT
                     PESAKTIRQWYAYYIPKLTSGEINKETFVLRGELQYKRKVGEPIWVEMTVKILIDESG
                     KIIGIHGTSVNIDERKQTEIELRASREKYKSIFENIIDVYYETTLDGIILEISPSIEK
                     VSKYKREELKGTSLISFYKTPESRANFIDAIMNMGFVSNYDLILLDKDGREIDCSITA
                     RLLTDENGQPCKICGTVRDTTEKKRVEKLIKESEERYKTLVSLSPNPVIVTRNGYFYY
                     VNPSAIRFFGFSAPEELLGRKMIDFIDPKTNDKVINEIAKLSESENCPALELGIINKE
                     GKKLTTLSAIKTISYEGEPSLLFIIQDITQQKKYEEELFQAKQKAEESDKLKTAFLAN
                     MSHEIRTPLNGIIGFSGLLKRQNLEKEKQEKYAEIIHSSSHQLLAIINDIIDISKIEA
                     GQMVVYHEPINILQIITELSYIYKSLAEKKDIEFRLSPLENIQEISSDEVKLKQILTN
                     LLNNALKFTHNGFVELGYKKQNHEVVFWVRDTGIGIPEEFQKIIFERFRQVEIASNKK
                     YGGTGLGLSISKALVQMLGGRIWLDSEVDKGTTFYFTIPYHTENKHTTPNIPHTPQNE
                     NINWSNKTILIVEDESVNSYYFEEILKNTGANLLFADKGMDAVEKFEKIKIIDLVLLD
                     LKLPDIDGFEVLKQLKKMNPDIKVVAQTAFALSNDKKAAINAGFNGYIAKPVEKNELF
                     ETISKLL"
     gene            6105..9356
                     /locus_tag="PK252_03145"
     CDS             6105..9356
                     /locus_tag="PK252_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT13737.1"
                     /translation="MKILKTSCLLILSLIAFFQRSTAQTRLLRFPTVSEKSIAFSYGG
                     DIYTVDINGGNARKLTNHVGYECFPRFSPDGKWIAFTGQYDGNTEVYLIPSEGGTPKR
                     LTYTATLQRDDIGDRMGPNNIVICWTPDGKNIVYRSRQHSFNDFKGKLYKVSIDGGLS
                     EELPFSVGGFCSYSPDGNQLAYNRVFREFRTWKRYRGGMADDIRIFNFKTKETINITN
                     NDAQDIIPMWIDKDIYYTSDRTGRLNLYKYNTSTKNTEQKTLFTDFDVKFPSSNGSLI
                     VYENAGYIYKYDTKTGQNEKVDITVSDDKLWARDEIKNTSTYIRSFDLSKNGERVVFS
                     ARGDIYSLPATKGITYNLTRTPGVHERNVTWSPDGKYIAYLSDATGEFEIYIQPVDGS
                     SQAVQLTKNADTYYYEMVWSPDSKKILWADRKFRLRYIDVATKAITEITQSKYGSVGD
                     YKWSPDSRWVVFAQEADNHFSIITVYDTKNKSLHDITSKWFFSHEPSFSPDGKYIAIS
                     SARDFTPSYSDIEWNYAYFNMEKLYLILLKKDGKSPFAPENIEVTTDTSNTDKKKPAE
                     TKEKKSETAVEIDFENIEQRLIPVPVKASSYYNVWMAGDGIYYNERSEGKTNTFKYYD
                     LKKKEETELGTANRYAISSNGKKMLVNESGKWGVIDLPTSKISLKDEIDCSNMMANVN
                     YKKEWRQIFDEAWRQMRDFFYVENMHGLDWKAVHDKYAPLVDFVGHRDDLTYIIGEMI
                     GELSIGHAYINSGEKPKAPRINTGLLGAKLIKDNSGYFKVTKILQGANWNKDLRSPLT
                     EPGVKIAEGNYILAIDGVDIKQCADIYEMLYNKADKTIALTVNSKPEITGSWQTLVIP
                     VADESNLYYYEWVQKNLKLVESATNGEVGYIHIPDMVSEGLNEFVKHFYPQLQKKVVI
                     IDDRGNGGGNVSPQILERLQRVPYRMKMHRNATVPGTVPNETMTGAKIMLIDQYSASD
                     GDLFPYGFKKLKLGKVIGVRTWGGVVGISGSLPFIDGTDLRKPEFASYSSDSSQWIIE
                     GHGVEPDIVVENDPAREYEGIDDQLNKAIELAKQLAKEQKPLPAIPTAPDKSK"
     gene            complement(9473..9967)
                     /locus_tag="PK252_03150"
     CDS             complement(9473..9967)
                     /locus_tag="PK252_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13738.1"
                     /translation="MKVRFYISALLAFFFLGYAAKAQGALPEMNKKIIEFVTENIGKK
                     VDRGECWDLANQALILVDAKWDHQYKYGKRLDPNKDEILPGDIMQFEKVKVKYVVVNG
                     NETTTTIEDMGHHTAIIYRVIEPGVFELAHQNTSFSGRKVGLSKLELKNITKGKYFIY
                     RPTK"
     gene            10296..11297
                     /locus_tag="PK252_03155"
     CDS             10296..11297
                     /locus_tag="PK252_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT13739.1"
                     /translation="MTALMGLIFLLSATIFYLVIKNKKLRTLSSEQNLQIVLLKKDLE
                     NTNDEVKKLLKEINKSGLYIRLVQQSPNAIMLMDKDANVIWTNKGFEQMYEYTYKEFI
                     AARGGNYRKTSFHPAVHERLDKLARTKKPIKYEALNITRTGKELWTQTALMPILGDDD
                     ELIGMVTIDTDIHQRVVESDSVMEKMEDINKKMDEIFVYFKAMKKESRDLFESINNSK
                     QWIEQTDEIVKFIKEISDKTKILGINASIEAHVAGSYGVGFRVISNEIVEISHQTTNS
                     VAEISKILENIKKSHDGLLQNKHDTEKTMELYNEKMKDLKKEIIEVENAIQMFKTLT"
     gene            complement(11396..11863)
                     /locus_tag="PK252_03160"
     CDS             complement(11396..11863)
                     /locus_tag="PK252_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT13740.1"
                     /translation="MVSPQSFSEKEAYDFAARYCSGQERCKAEVVQKLKSFDVSEDII
                     NQIIEKLCQEKYLDDKRFAKAFVHDKLRFNKWGRIKLSMALRQKGVEESVANAALKEI
                     DQNEYLDILKEELDKKAKFVKGANKEVTKKLFQFALSRGFESYVIQDVLSMED"
     gene            complement(11867..12793)
                     /gene="prmC"
                     /locus_tag="PK252_03165"
     CDS             complement(11867..12793)
                     /gene="prmC"
                     /locus_tag="PK252_03165"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT13741.1"
                     /translation="METSSVKLSEALQYFEKEISALYPENEFRSIRRIVFDKIANIHP
                     VDFHLKPNLEVSAESFLKIQEVTEGLKEFRPIQYLLGSADFLGLKLKVAPGVLIPRPE
                     TEELADWIIKTHKFNENRILDIGTGSGCLAIALDKLMLHSQTDGIDISDEALEIAEEN
                     SFLNESIVNFFHYDILQDFTTESSKHFKEAYDIIVSNPPYVTKSEAAQMQKNVLDYEP
                     HMALFVEDEEPLKFYKAIADFARIKLEWGGFLYFEINPRFHKEIMEMLTLKGFRNVQV
                     KPDLSGKPRMVRAQIKLFKKGENPELEKFRQF"
     gene            12951..14018
                     /gene="ribD"
                     /locus_tag="PK252_03170"
     CDS             12951..14018
                     /gene="ribD"
                     /locus_tag="PK252_03170"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT13742.1"
                     /translation="MDTKDEKYHLRTLQLALNGLGRVAPNPMVGSVIVLDNKIIGEGY
                     HQHFGGPHAEVNAIASVKDEKLLRKATLYVNLEPCAHYGKTPPCAELIIKKQIPNVVI
                     GQLDPFPAVSGKGLQMLIDAGINAVVGEHVDQCRFLNRRFLTFHEKKRPFFILKWAQT
                     IDGFIDIVRNHNSPQAPNWITDEHTRILVHKWRSEEQAILIGTNTALFDNPRLNTRDF
                     YGKNPIRLVLDRQLRLPKTLHVFDKSVKTFIFTEKQKKSEENLEYIQIDFSKLLYELS
                     TFCYERQIQSVIVEGGARLLNTFIQQNLWDEARVFIGNTSFGKGIAAPSMNSMPVSSE
                     NLPNSMLKIWHKHPAAFNIDK"
     gene            complement(14047..14445)
                     /locus_tag="PK252_03175"
     CDS             complement(14047..14445)
                     /locus_tag="PK252_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT13743.1"
                     /translation="MKISLAHVAIWCRDLETMRQFYEKYFNGKSSTLYINKNRMFQSY
                     FIEFVGNTRIELMHKPNIAEIPVSTNVQHFGLAHLALGVDSKENLEELTERLRKDGYY
                     IASEPRVTGDGYYESIILDPENNIVEISYK"
     gene            complement(14464..15801)
                     /locus_tag="PK252_03180"
     CDS             complement(14464..15801)
                     /locus_tag="PK252_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT13744.1"
                     /translation="MKDIVVKASMAIAFLLLSSVIFSQKAMTLQECISYALENNLTLK
                     RQELQTQISKNNYKQSYFNLLPDLGAGGSHNIGRGRVADYSSFAYSNNLNSGSVGLSS
                     NLTIFSGFQNLNTIRADYYSFLSANESLKKARNDISLSIASSFFQILYDKEYFQVLKK
                     QYEASKLELENTNAKYAVGSIARGAQLEVEATAMTNLSSLTEAQNRLNMSLLTLAQML
                     DLDTVKNFDIVAPPILAVPDTFAENADSVIDVALNTLPQIKGAEYALKSAERRYAVAK
                     GAFSPQISLTGQYYSQYNLDEKTLTGEKVPVNDQLDQKLYKQLSINLSVPIFTRFQNV
                     KNVKNAKLNKLDAEYSLQQSKLGVRKEVEQAFADAKASYESYKAQTKAAAAQEENFKN
                     IRQKYELGLISHIDFVVAQNNYLKVQSDLLQAKYKFIFNLKILDFYKGIPIVL"
     gene            complement(15940..18288)
                     /locus_tag="PK252_03185"
     CDS             complement(15940..18288)
                     /locus_tag="PK252_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13745.1"
                     /translation="MRKIKLLILFICAAFNVALSQTITQTVKGKVYDSESQAALPGAS
                     VVVLNTNPGLGNIADVDGLFKITGVPIGRYNIQVSFLGYETVIIPEILVSSGKEVVLN
                     ISLKQSVTQMDEVKVKAFTRKDRPLNTMAAVSARSFTVEETRRYAGGLDDPARMASAF
                     AGVTVGSVQDNAITIRGNSPKGVSWRLEGVEIPNPNHFAGGNIAGGGVVTIFSSQLLA
                     NSDFFTGAFPSEYGNALAGVFDMKLRNGNNERFENTFQAGLMGIDVASEGPLGEKGKA
                     SYLFNYRYSTTGILSNMGMIPSQQVPKYQDLSFKFNFPTKTAGTFSFWGIGALDENNE
                     PVDKDSMVWESSWDKVKYNWGLDIGAAGLNHKMILGNQSFVSTTVAVSGTRNSFLEEM
                     LDKSMNLQPSTKLKDNSWRVTMSSYINHKFNAQTTLKTGVNYHEIFYNLDISGIMNEA
                     LSTFQNIVKENGHSGLAEFYAQAKYEVSSRFTFNGGINTNYFLLNDNYSVDPRLSASF
                     EISPKQTISLGYGKHSQLEELKFYFISENINGSKAYLNKDLDFSKAHHIVLSYDWRIT
                     DKLRLKVEPYFQYLYDIPGIADSSYSMVNFSQDWTFREKLENNSTGRNYGVDLTFERF
                     LNNNFYYLVTTSVFNSEYKADDGVWRNTRFNKNFTANLLVGKEFFTARNKVIGINARF
                     NYIGGERYSPLNEAESIVQQRVVSDEAKAFEKQFPATYYLDFTLTYRTNHRRFSGTWA
                     LQVKNALGSPNQGGYYYNIKTKTIEKDTYAVVLPVLSYKIEF"
     gene            complement(18493..19812)
                     /locus_tag="PK252_03190"
     CDS             complement(18493..19812)
                     /locus_tag="PK252_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13746.1"
                     /translation="MKRFLVLVLVGLFSFNAFSQDDSAKMQRISPIQISFVPYFSFNP
                     VSGADAVCVSINCIGGYVPAVAAFEVGGVFNIVKSDVNGAQVAGVSNFIGGNVVGVQI
                     AGVSNVSKNVRGSQISGVVSFNRQSVVGSQVSGVASITGGRVEGIQIAGVSNVMVDKG
                     GDSSVVKVQQITGVSNVAVSSIMGVQVAGVSNVSKDMTGAGVAGVSNTSRNLFGCQVA
                     GVTNQAFGKANGTQVAGVANVANEIKGSQVAGVVNVARKVDGVQVGVVNIADTLNGVA
                     IGLVNLSRNGIRQFEVSTDEKYNVNLAFRSGAKKLHGILLLGVDPSTLDAPIWAFGYG
                     LGTTFEYNERLSFDLDVSSQQYYKRWDSDWHNQLYKIYFGVDRKLASKLSIAAGLTAN
                     LQYVGSDQSQNHKYFSDYAPYKIAKTKWPSGRELNFWIGGKVAIRFF"
     gene            complement(19910..21910)
                     /locus_tag="PK252_03195"
     CDS             complement(19910..21910)
                     /locus_tag="PK252_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13747.1"
                     /translation="MKIKLCILLIFVAISIVRSEIPVLERQVTINGNNMSVDEVLDKI
                     SVQAGVTFSYSSSVIQEIRPVTINCYKQPVRYVLSLVMGDKVLFRSRGKYIILKQNKN
                     AGQIQKKLVVEGYITGADNEGLLKDVTVYDKNSLVSVNTDEYGYFKMLVPASDSAAQL
                     IVSKSGYDPGQIQPIVVHNKVVTMPLKKQQEDTNKIVNKINIPAWLLSDKLKTNAANI
                     KDEVFKKVSFSLLPFVGTNKLLAGSVSNDMSFNMTIGYVKEVNVAEFGGIMNIVSGNA
                     RFIQCAGVANVVGDSVLGYQAAGVFNVCKYVQGCQTAGALNIVESAKGVQLAGVGNLV
                     VKDAGVQVAGVLNITWGNAPSQISGVVNVSKDSYLQLSGVINVGKNVYGPQLSGCINI
                     LERTTGSQISGLINVVSKDIQTQVAGISCHAKQVKGAQVSGFLNTSGEVQGAQVAGFL
                     NISKKNKGVQAAGLINISDTVEGMQMTSMLNIAEKVDGSQLALINIADTCTGVPIGLF
                     NYVKNGYHKVEVYADELLNVNLAYRTGVKKFFTFFTVGANAFYVSDPVWGYGIGLGTM
                     WQLNEKNDIELNIMEQNIVHKSDVENNAQAFRGYLGFNRKISSNSYLAYGLTYNLMYY
                     NTDDASSSSTFSKIIPYTLSSSNVSTGHKLSSYIGARVGIRF"
     gene            complement(21907..22923)
                     /locus_tag="PK252_03200"
     CDS             complement(21907..22923)
                     /locus_tag="PK252_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT13748.1"
                     /translation="MKLDSKHIDYLIARYLTGEATRSEMQQLERWMEEAGENKKYFEG
                     IRFAHDKTVASHRIEKFDVDKGWNSLHKKMQAADTQPKKKAKRVFFSSNLFLRVAAVA
                     VILIGIGTVAYYYGANKVDISEQLSLSAFDSVVCRKMLDNSIVRLNKNAKIIVKKGFG
                     KEHRTVELKGEAFFDVQHDTLPFIVQTEKAFVKDLGTSFNVKADEGSDTVEIYVQSGV
                     VAFYTQYNPGIIVNEGEKGFYIKSQDNFYKRAVNKANIAYARIFHFDNSRLVDVVTQI
                     NSVYGQYITLSLGNIYNCRITVSFENETIENIVNIIAETLGLRVEKVAGGYLLTGEAC
                     NNMP"
     gene            complement(22928..23506)
                     /locus_tag="PK252_03205"
     CDS             complement(22928..23506)
                     /locus_tag="PK252_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT13749.1"
                     /translation="MVLVTDSEFVAGIRNSDEAAFENVFRSYYERLCNYANTMLYDVD
                     EAEEVVQNMFLALWEQRDSIEIHTSVKSYLYRAVHNSCLNRIKHNKVKQMHGEHVRYN
                     TESSIEGTSQMVIGKELEQQINAAIESMPQQCRTVFVLSRFENLTYAEIAEQLEISIK
                     TVDKHMVKALKILRERLKEYLPLLLWLLINKN"
     gene            23725..25176
                     /locus_tag="PK252_03210"
     CDS             23725..25176
                     /locus_tag="PK252_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT13750.1"
                     /translation="MKNFFKIFCLLAAFSVFAVSCDDSDDDSMSELDLSINYFIWENM
                     NLYYLWTDNVSALDEKNYSSKEDVENNLLKKYPDHEEFFNSLLYKKGEVDRFSWIVDD
                     YEKLEKMFQGITKSMGFDFVLGKIGTTDNVFGLVRYVVKGGPADRAGLKRGDFFTRID
                     DQQLTVSNYNTLLSNENSYKIGLAEVIDHKITNLSTSYNLTAEEVHENPIYLDTIYTI
                     KDKKIGYLVYNGFMSNYDNQLNAVFAKFKQAGIQDLILDLRYNGGGSLASAKYLASMI
                     YSTDTTLIFNKNSYNADLEKYIIEKEGKNALNEYFAAKIKASDDTREEAINSLGLKSL
                     YVITTSSTASASEILINDLKPYIKVTMIGGTTYGKYVGSITIKDYDRNDKLNPNHKWA
                     LQPIIMKSTNKNGVSDFYNGLTPDEKLEEDITNLLPFGNTEELLLKAAIAKINGTSSA
                     KKAQAPVFSTFAERKDLIPHSREMHLNDVFKMK"
     gene            25177..25779
                     /locus_tag="PK252_03215"
     CDS             25177..25779
                     /locus_tag="PK252_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOT13751.1"
                     /translation="MDKRDFIKTSIASLGLLFIPKSAWPLEYYPTTENKEWAILYSTW
                     CGSARDASVWISEGMNGIANVFDVRENPDLSEYKYLIVGGAIRNGETSKELQAYLSKH
                     KDSFKNKIKGLFAVCGNMRQPVTTSQYTNFFDNHLIPLTRANKPLSRVFLGRITLNLM
                     EPKIRQQMKSFPNMEDYDNLKRSECMDFGREILNNFNANT"
     gene            complement(25879..26451)
                     /locus_tag="PK252_03220"
     CDS             complement(25879..26451)
                     /locus_tag="PK252_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13752.1"
                     /translation="MNKKIQILLVVFLGFTNVIKSQSKENAAYSTNIEIFINKPGVAI
                     EKEFIELGNLDEIDVRLLKAKDLKSGGVCFALQFEYQSVKPIMPFSMVTFIDRDELEA
                     LTDALKLFQSIITQIDKSNNNKELKFMSRSGAVVGCFFKRERCKWMTYFQLKYNESKS
                     TVVIENDGFDFFMSLISLAKDKIEAESSGK"
     gene            complement(27113..28849)
                     /locus_tag="PK252_03225"
     CDS             complement(27113..28849)
                     /locus_tag="PK252_03225"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT13753.1"
                     /translation="MSNNELDLVKKRAEEWLQGSYDEATRQEVKKMLAGDEKELIDAF
                     YRDLEFGTGGLRGIMGVGTNRMNIYTVGKATQGLATYLKKNFPDLPVIKVAVAHDSRN
                     NSRLFAETTARIFAANGIKVFLFESLRPTPELSFSIRHFGCQSGVVITASHNPKEYNG
                     YKAYWDDGGQVVPPHDKNIIKEVEKTSISEVKWEGNSQLIELVGKEVDEIYTDKIKAL
                     SLSPEAVAANNQIKIVYTPIHGTGVTLVPMVLKKYGFTNIISIPEQNIADGNFPTVKS
                     PNPEESAAMSMAIEKAKETGAELVMATDPDADRVGIAVKNDKGEVVLLNGNQTGSILI
                     YYLLTRWNELGKLKGKEYVVKTIVTTDLIARIAKGFNVEYFDVLTGFKYIAEIIKQNE
                     GKKIFVGGGEESYGYLAGEFVRDKDAVMACALIAEAFAWAQSKGKTLYDILMEIYVKY
                     GLFKEYLVNVTKKGKEGQEEIARMMESFRQNPPANLGGSKVAIVMDYQSQQTTNKITG
                     ETSVINQPKSNVLQFVNEAGDKVTVRPSGTEPKIKFYFSVSASMKDKSAFTETNAELE
                     KKIAQITKDLGL"
     gene            complement(28875..29741)
                     /gene="rfbD"
                     /locus_tag="PK252_03230"
     CDS             complement(28875..29741)
                     /gene="rfbD"
                     /locus_tag="PK252_03230"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT13754.1"
                     /translation="MKTILVTGAYGQLGNEIKDLVSFLPQAEFIFSDVDTLDICNKES
                     LTEYFNKHNLDFVVNCAAYTAVDKAESDQEKANMINAMAVKFLAEVCSQNKVKLIQVS
                     TDYVFDGTQSTPYIETDKTNPQSSYGRSKLDGELALKDNENAIIIRTAWLYSKYGNNI
                     VKTIRKKGSELGQLKFVFDQVGSPTWAHDLATAIIEILKLSIENEKNFKPGIYHYSNE
                     GVCSWYDFAQEVVRISNINCKVLPILTSEYPVPAKRPAYSVFDKSKIKNSYNISIPWW
                     KDSLANCIKEME"
     gene            complement(29759..30328)
                     /gene="rfbC"
                     /locus_tag="PK252_03235"
     CDS             complement(29759..30328)
                     /gene="rfbC"
                     /locus_tag="PK252_03235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOT13755.1"
                     /translation="MNVLPTPIQDLVVIEPRVFKDDRGYFFEQYNKEKMLQKGLKYEF
                     IQDNQSKSTYGVIRGLHYQLAPYAQTKLVRAVVGKIFDVAVDLRKNSPTFGHWYGIEL
                     SDENFLQLLVPKGFAHGFSVLSETAIVHYKCDCLYNPQAERGINFADKTLNIDWKVEM
                     ATAAVSAKDRVHPDLEKAEMNFVFGSSEL"
     gene            complement(30335..31285)
                     /locus_tag="PK252_03240"
     CDS             complement(30335..31285)
                     /locus_tag="PK252_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT13756.1"
                     /translation="MSKILVSGGAGFIGSHLCERLLKEGNDVICLDNFFTGDKSNVAH
                     LLGNPYFELVRHDVTVPYYAEVEQIYNLACPASPVHYQYNPIKTIKTSVMGAINMCGL
                     AKRTKSKILQASTSEVYGDPEVHPQTENYWGHVNPIGIRSCYDEGKRCAESLFMNYHF
                     QNDVKIKIIRIFNTYGPRMHPNDGRVVSNFIVQALQNKDITIYGDGKQTRSFQYVDDL
                     VEGMIRMMNVTPDSFTGPVNIGNPGEFTILELAEKVIKLTGSKSKLVFQPLPQDDPMQ
                     RKPDISLAKEKLNGWEPKVALDEGLLKTIEYFSKKMNIKK"
     gene            complement(31298..32626)
                     /locus_tag="PK252_03245"
     CDS             complement(31298..32626)
                     /locus_tag="PK252_03245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT13757.1"
                     /translation="MKIAIVGTGYVGLVSGTCFAETGLDVTCVDVDAQKIEKLNNGQT
                     PIYEPGLDQMIERNMAKGRLRFTTSLADAIADCEAVFIAVGTPPDEDGSADLRYVLAV
                     ASDIGKYMKNYLVVITKSTVPIGTSLKVKKAVQDSLDARGVSVKFDVASNPEFLKEGN
                     AIEDFLKPDRIIVGVESQDAEKIMRKLYKPFLLNNHPILFMDIASAEMTKYAANAMLA
                     TKISFMNDIANLCEIVGADITWVRKGIGSDPRIGSKFIYAGAGYGGSCFPKDVKALIK
                     TGEEKKWPLEILKAVEEVNERQKESLVKKIMKHFNNNVSGKTFGIWGLSFKPQTDDMR
                     EAPSLIIIENLLKNGANVKVYDPVAMEEAYKYLGEKVVYSKDQYDALIGADALVLVTE
                     WNEFRLPNFEMVAKLMNNKLIFDGRNIYEPSEMAEMGYTYYSIGRKPVFN"
     gene            32881..34836
                     /gene="rpsA"
                     /locus_tag="PK252_03250"
     CDS             32881..34836
                     /gene="rpsA"
                     /locus_tag="PK252_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOT13758.1"
                     /translation="MAKKELEKEEFNEELLSNEIVDNEEIVDDVEEVEEDFDEVDDDS
                     LKTIVELKKAQHQKETAEFDWNNYTNKGEKYAADVKAQFEAMYDKTLSTIAENEVVEG
                     TVISMNKREVVVNIGYKSEGVVSLNEFRYNPDLAIGDKVEVYVESQEDKKGQLLLSHK
                     KARALRSWDRVNEALEKDEIIKGYIKCRTKGGMIVDVFGIEAFLPGSQIDVKPIRDYD
                     AFVNKTMEFKVVKINHEYKNVVVSHKALIEAELEQQKKDIISKLEKGQILEGTVKNIT
                     SYGVFIDLGGVDGLIHITDLSWGRVNHPEEIVQLDQKLNVVILDFDDDKKRIALGLKQ
                     LTSHPWDSLSADLKVGDKVSGKVVVMADYGAFIEIAPGVEGLIHVSEMSWSQHLRSAQ
                     EFLKVGDTVEAVILTLDREERKMSLGIKQLKADPWEKIEEKYAINSKHKAKVRNFTNF
                     GVFVEIEEGVDGLIHISDLSWTKKIKHPAEFTSIGEEIEVVVLDIDKENRRLSLGHKQ
                     LEENPWDVFETIFTPDSIHEGTIVEIFDKGAMIALPYGVEGFATPKHLVKEDGVMAKV
                     DEKLEFKVIEFNKTAKRIILSHSKVYEDAKKAEETAAKKAEEPATRKAGKKVKANIEK
                     TTLGDITELAALKTEMEENQKKGKQAE"
     gene            34869..35222
                     /locus_tag="PK252_03255"
     CDS             34869..35222
                     /locus_tag="PK252_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT13759.1"
                     /translation="MEFKIEKLQNYTIIQVLEEKLDTHIAPSLKSELVLISGNGEKNI
                     ILDLSNCKYCDSSGLSAILVANRLCKNAGGKFVLTGLNDAVERLIIISQLDTVLNITE
                     TINEAVSLISDNKAN"
     gene            35233..36144
                     /locus_tag="PK252_03260"
     CDS             35233..36144
                     /locus_tag="PK252_03260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOT13760.1"
                     /translation="MSLSVTILGSSSAIPTSKRNLTAHLLNHDERFFLIDCGEGTQMQ
                     LQRFRTKYLRINHIFISHLHGDHIFGLFGLLSTFSMMGRKNTLHIYSPGRLKDLLNSH
                     FAFMGETLNYQIEYHEVDAKMPEVIYDDKKITVTTIPLRHRIPCTGYCFREHPKLLNI
                     KKECIEKYQLGVKDIVDIKQGADFVLENGIVIPNKELTTEPVPPKSYAFCSDTKALQR
                     IVPYIANVDLLYHETTFLNEDAKLAKATYHTTTGQAAQLALEANVGKLVIGHLSSRYK
                     DDNRFIKEVQDIFPNAIMAEDGMKIDI"
     gene            36413..37837
                     /locus_tag="PK252_03265"
     CDS             36413..37837
                     /locus_tag="PK252_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13761.1"
                     /translation="MNSFEMEESNDKQLLLAQKFSSFVNSHKSFYFTEDEYEDIIEYF
                     KLHNNKELYYKSIIAANNQYPYNQEFKYFQIEYFLENKQYSEAQEYLQKSLGCLFDEK
                     NKIYYQAAIFYCTGHQDNAEALIKSTGIEGIDDFGEFYTELGELFMDIKRYDLAIKAF
                     SSAYLENHEYERLCDIFDASFEDIETFKEANSFDFLTEKLPFDANVWLKKYEYCLSIF
                     DYEKALEALDFSLALSPDEYLELKKVALLVYLERYSEAIKLFEQLNHDIRDEDETLYC
                     DEFYVVSFAYFKLKQFEKAEYYAMEDLRLNKLESNATAILLSDIYVKTGKRAKAKNIL
                     FDLLKKEEENIDILFRLSEIYEIEGNMNKAIEMMLKLLKNDSSDVNNFLRLANLYFQI
                     NLLSKAIRTLEESSNIEPTNANIHYSLAAYYLLQNKKDLSVKHLNYALNLSPSKFHTL
                     LDIFPEALQIEEIRTCFHKNNLRI"
     gene            37834..38622
                     /locus_tag="PK252_03270"
     CDS             37834..38622
                     /locus_tag="PK252_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOT13762.1"
                     /translation="MNFPLTNIPLRESKPRNNGITMIMDKGLSLREAENLISSCGSYV
                     DLVKFGFGTGLITMNLEEKIKLFSEANIKPYFGGTLFEIFLVRGLFDDFRKFIDKYKV
                     EMIEISDGSISLPHEEKLKYINILSKQATVVSEVGSKVAGVVISPEKWVEMMKHELQA
                     GSWKVIAEARESGNIGIYNPDGSANKELINDIVKHVNTDNIIWEAPNKPQQVWFIKLL
                     GANVNVGNIAPNEVISLETLRLGLRGDTFFQYLPENVVKKYFVK"
     gene            38630..39385
                     /gene="aroE"
                     /locus_tag="PK252_03275"
     CDS             38630..39385
                     /gene="aroE"
                     /locus_tag="PK252_03275"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562393.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT13763.1"
                     /translation="MKQFGLIGYPLGHSFSKGYFAEKFKKENITDCIYDNYPIENIGL
                     FKDIINKNTNIVGLNVTIPYKEQVIPFLNEIDIEAKEIGAVNTVKVKRNGNTVILKGF
                     NTDVYGFETSLKPMLEKQHQKALILGTGGASKAIKFILRKLGISYISASIEELKENEI
                     RYSDITPKLISEHLLIINATPLGTFPKVDTCPDIPYNAITSQHVLFDLVYNPDVTLFL
                     KKGRDNGAKTKNGLQMLHLQAERAWQIWNSPEF"
     gene            39391..40335
                     /locus_tag="PK252_03280"
     CDS             39391..40335
                     /locus_tag="PK252_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546985.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT13764.1"
                     /translation="MHQFPWHHQRRFNSYAEYFKRHFGHRVQKLTIDAGFTCPNRDGS
                     IATGGCTYCNNEAFNPSYCTPHKSITRQLSEGIEFHQVRYRNAQKYLAYFQAFSNTYA
                     PLEILKQYYEEALAFPDVIGLVIGTRPDCVDAEKLDYIARLAQRYYIIIEYGIESIYN
                     TTLKKINRGHSFEQAVKAIEETKKRNIKTGAHFIIGLPGETEQMLFEAIPVISSLPLD
                     SIKFHQLQIFKGTVMASEYAANPKAFNLYSLEQYLELIVRVVEHLNPAIVIERIAGEA
                     PPWHLASTSWGLLRINDIVRLFEQKLEEKDTWQGKFYL"
     gene            complement(40336..41097)
                     /locus_tag="PK252_03285"
     CDS             complement(40336..41097)
                     /locus_tag="PK252_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13765.1"
                     /translation="MKNNVLTLLLAFILQIAAMAAESNEALFNKGNEAYKKGKYDTAL
                     IFYKAIDSLGYKSSELFYNMGNVFYKKKDIAHAILYYERAHKLSPSDEDITFNLQLAQ
                     ALAVDKINTLPEFFVSRWWRNFSELASTDAWAIWSIVTFCISLFCVLLYLFVNILFVK
                     KTAFTCGIILFLFSGIAFSHSLHTKRLIEGQQQAIVMSETVTVRSSPDKESTELFVIH
                     EGTKVIIIDQVGEWLRIKIADGNNGWMLASNVEKI"
     gene            complement(41130..43040)
                     /locus_tag="PK252_03290"
     CDS             complement(41130..43040)
                     /locus_tag="PK252_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOT13766.1"
                     /translation="MINIFKNISVNMKSVIPIAFDGLIKKRMSLLALLLILSLSIMAE
                     NIQFVASAPRVVEVGEQFEITFTLNAQPSGFRPPEFKGLNLLGGPSTSSSSSVQFING
                     KVTQSTTFSYTYYFSANNPGTYTFDPAKATVDGKSYMSNSITIEVAGKNNTTRQSNAS
                     NQQQNQTQQSQQSLSDVDDSGSDDFFVRIIVDRNSISRGDHIVATIKLFSKHSISSIE
                     NVEYPSFGGFYRQDIETPQLSKLEKEVVNGQVYYTGVLQKMVLFPQKNGEIVIDPFSL
                     QAVLQIVMRNRSRSVWDDFFGPQVREVRKKVKSNPVKISVSPFPAGAPADFKNIGGSY
                     TFSATVNKNTVKTNDAINLKVVIAGSGNIKLIEPCDIKFPADFETYDPKVTVSSQVTT
                     AGVKGSKTFEYLIIPRHAGEYTIPSFSFSYYDTKLKQYKTLSSGDINIKVEKGANDNT
                     VEVVSGIDKQDIKFIGKDIQFIKTQTPSFRKKGEYIFGSALFILGYLLPLLLFIVAVI
                     IGRKKIKENANIRLVRNKKANKMAQKLLKEAKTSLHDNNGEQFYELVLKAMWGYVSDK
                     LGIPVAKLSRDTVTEYVQNLQLDEQVVVKFISVVDRCEMARYAPTGGSAEMKQVFDDA
                     VDVITKVEQHYK"
     gene            complement(43053..43742)
                     /locus_tag="PK252_03295"
     CDS             complement(43053..43742)
                     /locus_tag="PK252_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT13767.1"
                     /translation="MMKLIFIFISLFLVVHALAQNDKKLVRKGNKEYTNKKFNEAEVD
                     FRKALEEQGSYIPTATYNLGNALYRQNKFDEAVNAYTHAADAKIDKKDIAKAYHNLGN
                     TYLQMQKFQESIDAYKKALRNNPNDMESKYNLAVAQKLLKDKPQNKQNQQNQQDKNNQ
                     GQQNQPQNNNQDQNKQEQQQQQPQQQQGQMSKEQAQQLLRAIQDDENNTQEKVKKEKA
                     KGQKTNPEKDW"
     gene            complement(43744..44787)
                     /locus_tag="PK252_03300"
     CDS             complement(43744..44787)
                     /locus_tag="PK252_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT13768.1"
                     /translation="MFRFENTYMLYVLLLIPILIGVFHWIRIQRSKALARFGESELVE
                     QLMPEFSKTKPIWKFYLFLAAVVFVILGLARPQFGSKLQEVKRKGIEIMIALDVSNSM
                     NAADIEPSRLEKAKNVISKITDRLTDDKIGLIVFAGEAYTQIPITTDYVSAKMFLNHI
                     NTGMVNVQGTAIGKAIRMAENSFSPNSELQKVILVISDGENHEDDAIEEAKIANQKGM
                     LVYTIGMGLPEGAPIPEGGPSSLNFKKDREGNTIISKLNEEALAQVAAAGGGDYIRGN
                     NTQAGINKLFDRVDKLNKKEYHAQIYSEYDDQFQYFFAMAIILLIIEFLLIEKKSKML
                     VNFKLFEVKKIRR"
     gene            complement(44799..45782)
                     /locus_tag="PK252_03305"
     CDS             complement(44799..45782)
                     /locus_tag="PK252_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT13769.1"
                     /translation="MVFANPKMFFLLLLIIPAVIWYVRKAQKMSASVVFSNLQGLANV
                     KKSMRAKLRHVVFAANMLAFALLVVAIARPQSTNQWKNVSSEGIDIMMALDISGSMLA
                     ADFKPNRLEAAKEVAIEFISGRPDDRIGLVVFSAESFTQCPLTTDHSVLINLFHDVKM
                     GMIEDGTAIGSGLATAVNRLKDAKKAGKVIILLTDGVNNQGSIAPVTAAEIAKAFGIH
                     VYTIGVGTIGMAPYPVQTPFGTQYQDMEVNIDEPMLKQIAQMTGAKYFRATNNQKLRE
                     IYKEIDKLEKTRVEVKEYHKRDDVFMPFGIAALLLLLASFALKHTVLRVIP"
     gene            complement(45817..46806)
                     /locus_tag="PK252_03310"
     CDS             complement(45817..46806)
                     /locus_tag="PK252_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13770.1"
                     /translation="MQPHKKILFSIVLILLSAKIFAGGTISVDARFDSAQILIGDQMY
                     LNMVVTQPVGVKVMFPVFTDSIVSKIEVLSSLPADTTHIDANNIKVTKKYLVTCFDSG
                     LYQTQPLKFAFFNGTTTDTLVSAPIMLAVNTLQVKDPSKIADIKGVIEIPLTLKEILA
                     YTGIGLAILLAILLIAYVIWRWKTHKPILGIFAKPAEPAHVIALRELERIQREKLWQR
                     GFIKEYYSGITDAVRTYIEARFEVPAMESTSEEIISRLKTIDVIEKSLITKLTEMFSL
                     ADLVKFAKMEPAPNENENIWAVAYDFVKNTIPVVATEPSVNEKKEEPEGKDVK"
     gene            complement(46838..47719)
                     /locus_tag="PK252_03315"
     CDS             complement(46838..47719)
                     /locus_tag="PK252_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT13771.1"
                     /translation="MEAADLLKKVRRIEIKSRRLTKNIFAGEYHSAFKGKGMAFSEVR
                     EYQFGDDIRNIDWNVTARFNHPFVKVFEEERELTVMLLVDVSGSRHFGTNKQLKTSLI
                     AEVAAVLAFSAIQNNDKIGMIMFSDKVEKFISPQKGRSHTLRIIRELLTYEPKSRKTD
                     ISEALRYFTNSIKKKCTAFVISDFFDETNDGVINFSEALKIANRKHEIAALQVVDRRE
                     AELVPVGLMKFVDAESDVETWIDTSDPEVRQQYALWWMKRQERLQNLFSKCGIDTVQL
                     YTGEDYVKPLMKLFEMR"
     gene            complement(47732..48139)
                     /locus_tag="PK252_03320"
     CDS             complement(47732..48139)
                     /locus_tag="PK252_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13772.1"
                     /translation="MKTLAMLLGLISLVVQVEPVNYIGKTKTEIISLMQKNNPGFDLD
                     EGAVNNTYKYIKFVDKYNEETYLFFLDEKDECTFTKLMSDYSNMKLRTAELDKMYKKA
                     GDTKWIYVEKGTVYIIELIKDEWYFTILMKKKK"
     gene            complement(48243..49238)
                     /locus_tag="PK252_03325"
     CDS             complement(48243..49238)
                     /locus_tag="PK252_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT13773.1"
                     /translation="MNIAVNVKELNDRIQQESAFVDILSMEINKVIVGQKHLVESLLI
                     GLLSDGHILLEGVPGLAKTLAINTLAQAIDAKFSRIQFTPDLLPADLIGTMIYSQKNE
                     EFTVRRGPVFANFVLADEINRSPAKVQSALLEAMQEKQVTIGAETFKLPQPFLVLATQ
                     NPIEQEGTYPLPEAQMDRFMLKVVITYPKKEEERLVVRQNMGQTFPKATQVLKPEDII
                     KAKTVVRDVYMDEKIEKYILDIVFATRFPSEYKLERFQNMIAYGGSPRASINMAMASK
                     AYAFIKRRGYVIPEDVRAVCHDVLRHRIGLTYEAEAENITTEDVITEILNTVEVP"
     gene            complement(49650..50549)
                     /locus_tag="PK252_03330"
     CDS             complement(49650..50549)
                     /locus_tag="PK252_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT13774.1"
                     /translation="MVWVLLAILSALLLGVYDIFKKKSLVDNAVIPVLFFSSLTSALI
                     CLPIVLYAYFGNVPSDSVFYFRSLTLQAHVLIFIKSVIVGSSWTLAYFAMKHLPITIV
                     SPIRSSGPLWTIIGALLIFGEVLTVWQWIGIAVTLFFYYLFTLSGKREGISFRTNKWV
                     LFMTVSTIIGSISSLYDKFLVHHYNRLAMQCWYHIYMVPLMLVLFFIIWYPKRKQFST
                     FQWRFTIPLIGICLTVADFVYFWSLSHPDAMVAIVSTLRRGSVVVSFSLGALLFHEKN
                     VKRKGLILIGILIGIGIIILAGK"
     gene            complement(50679..51995)
                     /locus_tag="PK252_03335"
     CDS             complement(50679..51995)
                     /locus_tag="PK252_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT13775.1"
                     /translation="MRKLTLFFAFTLVVNGFTGVAQTAPKPYEVATWAGFRQAAISYT
                     FDDGCSNQFKVAIPLFDEYGFNLTMFTVKDWSGANWSALKTASDKGHEIASHTVTHAN
                     FSQVTITQQETELKDSKTAIEGQITNKKCLTMAYPYCVAGVDSICGKYYISARGCQGF
                     VEGKTPASYYNISSVICGDKGATQNLSQFKTNFSAVAKSNGWLVYLFHGIDKDGGYSP
                     ITSIELRKSVAYLAARKPKFWVTTFLNATLYSKERNAVSVTETATTDSSMTLQVTDNL
                     ADSIYNYPLSIRRPLPEGWPSASVTQNNVAVPFRIVQVDAVVYLIFDVVPDAGEVKIL
                     KSSTFVTPEVDIIPADDVDPVVVNRLDATKDDISAVYNNNRILISLPITVCSDFVISV
                     YDVRGVKLFYTNVSVASNEISVDVPSLKTGVYFVRQNVWSKKIAVS"
     gene            complement(52186..53058)
                     /locus_tag="PK252_03340"
     CDS             complement(52186..53058)
                     /locus_tag="PK252_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT13776.1"
                     /translation="MRRIINLIANHIHFVISPPLKGGARDGIVLVLFFSVFIGIPAQT
                     DTTILINNTKVEIVIPEKLKKGTILVLPGWNFPYNDVCLKSDFCKKALAEGFALVLPD
                     MQKSIYHLKVYPQTRADWVKYHTVYWLTDTVIPYIQQEYGLLKENQCNFLFGISTGAR
                     GVVMLAIQNARLFKAGAALSGDYDPLQMPNDNLLKGYLGEYNLHADLWKTTYNLVYQA
                     NNINIPLFLAHGKDDKVVNPEQSALLFKALMKSNEKAKHQFNLVEKSGHDYVFWQSQY
                     KAVFDFFTKCISKP"
     gene            complement(53058..53363)
                     /locus_tag="PK252_03345"
     CDS             complement(53058..53363)
                     /locus_tag="PK252_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT13777.1"
                     /translation="MAEKKSVMDELPVFHKKITVSGRVQGVGFRHTARIVAEQLNICG
                     LVRNMPDGSVFIEAEGTLPQLESFINWCYEGPPHAHVRHVAVIDGMVYGYTDFQITR"
     gene            complement(53342..53902)
                     /locus_tag="PK252_03350"
     CDS             complement(53342..53902)
                     /locus_tag="PK252_03350"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT13778.1"
                     /translation="MTSILEIEIDEKLSLREIQVTDAPAIFGIIDSNREYLRQWLPFV
                     DFTHKVSDTESYIKSILVHPYETRELTYVIIYENVIVGLIGFVRSDKANKRTEIGYWL
                     AEEYQGNGIVTTACQTLVDAAFRVLNVNRVQIKVAKENEKSKKIPQRLGFVFEGIERQ
                     GEWVNDRFYDLELYSLLRSEWQKRKV"
     gene            54254..54973
                     /locus_tag="PK252_03355"
     CDS             54254..54973
                     /locus_tag="PK252_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RPC7 family DNA-directed RNA polymerase III
                     subunit"
                     /protein_id="HOT13779.1"
                     /translation="MKKLLFIALAGIVLSSCAFHSGTISSGPIEKRVVYTDMAVGVAQ
                     TQKIFGIGGISKDALVLEAKRKLVQNRPLQEDEQYVNYTIDFKTSFWPFYSQTTATVS
                     ADVVTPANTDTKELYSDNYKKKLFAPTYNNDLFVVGDTIVYDNFFKGQILSFESKNKV
                     RIMYRTQNDKIRTKIVSLDDIYTQLKEYKTYKMGGNFYFDKVSTGGDNKVVNGKIWAV
                     GLKSVLVYDYNNKLCKEDFKQ"
     gene            55086..55313
                     /locus_tag="PK252_03360"
     CDS             55086..55313
                     /locus_tag="PK252_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13780.1"
                     /translation="MKTISVSISDLEYNQLGINNDKLSFTELVDIVNKEITKQTVNRC
                     IQLAEKYKFSRMSLDEINDEVKAVRDAKSNH"
     gene            55294..55524
                     /locus_tag="PK252_03365"
     CDS             55294..55524
                     /locus_tag="PK252_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOT13781.1"
                     /translation="MQKVIIDTNVLISALTQRNYPNFILYNYVLDNAVEVCISDELFK
                     EYLDVLNRPKFNKYPDFISKAEIVLTQIESKT"
     gene            55870..56133
                     /locus_tag="PK252_03370"
     CDS             55870..56133
                     /locus_tag="PK252_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13782.1"
                     /translation="MKKAKKQEVKAIEPVQVPNKFVRNKRKTILLNTMENEALDAYFK
                     KYKVQNKSRFMRETLMRVILNRFANDYPTLWDQPGVPAHLRPF"
     gene            complement(56251..57510)
                     /locus_tag="PK252_03375"
     CDS             complement(56251..57510)
                     /locus_tag="PK252_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT13783.1"
                     /translation="MSGYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFADQLEAL
                     EQASKRRGEEYVNLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTR
                     NMVTGASTANLALILVDARNGVVEQTIRHAYIASLLGIPHIVFCINKMDLVDYAQSRF
                     DEICSQIEAISSKLDVRDVWYVPISALKGDNVVERSEKMHWYNGPTLMHILENVHIAG
                     DINHTDARFPVQYIIRPYSDTYHDYRGYAGRIAGGVFKKGDAVVVLPSGLESVIKSID
                     VFNGSVEEAYAPQSVSVQLEDDIDISRGDMLVKPDSLPLVSKELDIMLCWLNERPMNV
                     GAKYHLRHTTADARCMISDVVFKVDINTLDELTGEKQLKMNEIARVRIKTTRPLAYDA
                     YKKNRITGSLVLIEEGTNETVGAGMIL"
     gene            complement(57607..58515)
                     /gene="cysD"
                     /locus_tag="PK252_03380"
     CDS             complement(57607..58515)
                     /gene="cysD"
                     /locus_tag="PK252_03380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOT13784.1"
                     /translation="MMKNYTINHLRELESESMYVIREVAAQFERPALLFSGGKDSIVM
                     FHIARKAFWPAKVPFPLLHIDTGHNFQETLDYRDKLMEDTGTQCIVRLVQDSIDAGKV
                     VEEKGFNASRNVLQTVTLLDAIEEFKFDAAMGGGRRDEEKARAKERFFSHRDEFGQWD
                     PKNQRPELWNIFNGRKHMGEHFRVFPLSNWTELDVWQYIYLENIPLPSLYFTHERDMI
                     IRDGVLLAQCPYIMLKPSDKLARMQVRFRTIGDMTCTGGVESPASTVEDIINEVAASR
                     TTERGTRFDDKRSESAMEDRKKEGYF"
     gene            complement(58705..59397)
                     /locus_tag="PK252_03385"
     CDS             complement(58705..59397)
                     /locus_tag="PK252_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13785.1"
                     /translation="MKKINFLIAILLFSLCVNAQKVNPNYVGVQIDTIYLFSPFGFVT
                     TIKNNERQTSPEFTQSVRDTLNKLILGQFPAKGIIINDSMLINDFKTQDNIIGTLEKI
                     KKIDNNVFPMVPIGKDLDSIISKYPGRYYGFLYYNGFMNNQIKRSMAASITLMVVTSV
                     LTLGMVTVYMIPLPSVIDATFVVVDKKEHKFLYYSNKVQTKSFSMEVNRHKLVDKILK
                     KYKKTIEVTNVK"
     gene            complement(59394..60632)
                     /locus_tag="PK252_03390"
     CDS             complement(59394..60632)
                     /locus_tag="PK252_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13786.1"
                     /translation="MMKIFLVIVLIDTLAKTSVFSQNIVPFEAEAKYGIKNKYTGKIL
                     IPAKYNRIQNYNDSVIIVQSRDFRYGAIDTLDNILIPFANEQLFIENYNPLHLGQIIE
                     SSNFNNEPEYSSWYYIDKNRNCIPYDYCPCPSWKKLSPNLSISSSYAQNGFKAQFNGH
                     LDSAFYWINMAIANEPQKASLYYYKAQFYLKDNKGAYLKHLDTLDILKKQIVDTSLSI
                     ALKYENRPVFVAQIMSLQSLFYKRCFIQKNKLEEVQTAAKSYDFMRYYEGLFFIGGLT
                     YNHGLEVETGFGRGSFTLNDKHQFNPYTVNSTIFYGGSWLRNFSSNIDGYKIYLISIL
                     KPLRFGICPILYTNYKKNLFVIRPEFGYGFNFLTISAGYNIRVADNGFPDLKTFNINL
                     RYYLPLFPNKSFSAQKPSKI"
     gene            complement(60663..61457)
                     /gene="ccsA"
                     /locus_tag="PK252_03395"
     CDS             complement(60663..61457)
                     /gene="ccsA"
                     /locus_tag="PK252_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT13787.1"
                     /translation="MTWLEFPYFAAVSALFYVAGSVFLYTSKNKLWGNAAIITGLAVL
                     GTFIGAFWAGLDRLPLRTLGETRLWYAFFLVLAGYLTYLRWGIKWIVPYSCGLALMFL
                     ILNIVFVEAHDKTLMPALQSPWFAPHVIVYMISYAVLAASALVSAKALFEMFFRNRHV
                     NDDVLKLADNLVSVGFTFLSLGLIFGAFWAKEAWGHYWTWDPKETWALLTWLAYLVYI
                     HIRFRHKQNENLAYFLLFISFVVLIVAWFGINYLPSAANSVHTYSN"
     gene            complement(61556..62800)
                     /locus_tag="PK252_03400"
     CDS             complement(61556..62800)
                     /locus_tag="PK252_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13788.1"
                     /translation="MAEVKRKTWEKPWGYKESAAIVAGLMIIGVMLDMLSAAIVEMPV
                     YPYNAILGLVFMALIPCVYFSFRKIYVVRWLSEVPACLGAIVLFSLIALAMAVVPQVL
                     EGQPTMVLKLNKLQGSWLFTISSIYLLTTLGLVVVRRISTFKVADIGFVASHLGMWIV
                     VMAAGLSSSDLKRFQVAVEEGQTVNMAYDKDYSWHTLPFAIKLDDFSIDEFPAKLALV
                     KSATMEIESKVKNNLPLIEKGKLFKLKNFSIEIVEYLPSAKADSSKGFVVSEDSLAVQ
                     AAFVKCFNNKTGQTKEAWINSSSPVQSAGVFYLDSTYLLAMTLPEPKKFSSKIEILVH
                     NDIKKNVLLEVNKPFAIAGWKLYQTGYDKEKGKFSKLSIIEAVRDPWVPAVYLGIILS
                     MAGIIISFWTKKKEKRLNTNGK"
     gene            complement(62965..63258)
                     /locus_tag="PK252_03405"
     CDS             complement(62965..63258)
                     /locus_tag="PK252_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT13789.1"
                     /translation="MKHYPPFIEKLGKVDSEFFQMVSAQHDKVLAPGAIDVKTKFLIL
                     LALDAYAGSSGVKGISDVARKMGATEAEIAESLRIAYHLAGNRVLHCAVNAFE"
     gene            complement(63353..63925)
                     /locus_tag="PK252_03410"
     CDS             complement(63353..63925)
                     /locus_tag="PK252_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT13790.1"
                     /translation="MQTLIDAIKRFVSLTDEDIDVIEQLFVKKELKENERLLTEGEVC
                     KEFVFVEKGLLRHCVNNDGKEVTFFFSAENEFVCDFESFIHRTPSKKTFYALEDTVVY
                     TISYNNMQLFYKKVSTGERFGRLYLEMTFAKAINHIVTTFTATAEQRYLNFLSDFRHI
                     QQRIPQYYIASFIGVTPQSLSRIRREMCKV"
     gene            complement(63970..64863)
                     /locus_tag="PK252_03415"
     CDS             complement(63970..64863)
                     /locus_tag="PK252_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT13791.1"
                     /translation="MSLRFKAHLALFAGNFLYGVNYVIAKKTMGYISPEAVIFLRILP
                     STMLFWAIAFITRNSMVNGRPEYKNLFIAGLLGVFLNQFLFIKGLSLSSPIDSAIIIT
                     SNPVFVLVMAALILKEKVTKLKVSGIIIGASGALLLAGSKGLVAYNPEHLTGDILLLV
                     NSASFAIYMMYAKPLMQKYDSVFVLKWTFLFGSLLYLPFGIKPMLAVQWSQLPMSIYA
                     ALFYIVICTTVLTYFLINYGIRHLSPTTVSIYIYIQPVVSVVMAFLTGTDTLNWINIS
                     AGLLVCFGVYLVSINRGGEKR"
     gene            65145..65723
                     /locus_tag="PK252_03420"
     CDS             65145..65723
                     /locus_tag="PK252_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT13792.1"
                     /translation="MIEKSDIELFKEIKEGKMSSYDKLFMTYYQPLCRFSLSFVKDEN
                     AAEDLVQDLFVHLWNNRAVMDITNSVKAFLYTSVRNRSLNYIKSQAARKRYETVHYEN
                     ISSDAVEEVEDNMPEKLVLIEKAINELPEKCREIFVLSRHKNMKYHEIAEHLDLSPKT
                     VENQMGIALKKIREFMAKHMSILILIFYGIFK"
     gene            65774..66748
                     /locus_tag="PK252_03425"
     CDS             65774..66748
                     /locus_tag="PK252_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT13793.1"
                     /translation="MHENVPYDLIASALEGACNAQEQNELDLWIAQSPANRAIYEEMK
                     SLWAESQQIADNFSPDAHQALARVHQRIGAGHKAKVFSLNSISWAYKIAASILIVAGA
                     LFFFVSNKKPVQLAAITDNKSNSEIVLSDNSVVKLNKNSKLTYPEYFKGKERRLRLEG
                     EALFDVTKDKEKPFVVEVGQTKVTVLGTYFNVRAYSLEDSIVVSLLEGKVEFAIEGKD
                     AKVILTAGEKGLYIKSKGSLTKMEEQNTNVTSWNTGILKFEALPLANVCTTLSEHYGI
                     SICIKNASDANLRFTGTFKNQRLSEILLSIEEATNLKAVKNGNSIIFE"
     gene            66769..67257
                     /locus_tag="PK252_03430"
     CDS             66769..67257
                     /locus_tag="PK252_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STN and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOT13794.1"
                     /translation="MQRILLFLLFTFIISVEIIAQRDILSRKVTVYAQNKRIADIIND
                     ISKQSEVDFLYSPDLVDLERQITVNITNVDLQKVLQQLFRGTNVIYSIVAGQVVLKKK
                     IQPPKHSISGFVSDMASGEKLAYATIAIQGKNFAVTSNNYGFYSLSEPEGFVYMIFVC
                     RV"
     gene            67409..>68595
                     /locus_tag="PK252_03435"
     CDS             67409..>68595
                     /locus_tag="PK252_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13795.1"
                     /translation="MQSLRQLPQLLGENDLMKSLQTLPGVQTKSEGSSGMLVRGGNHD
                     QNLYLLDGVPIYNINHLFGFVSVFNTDAVQHVSMYKSGFPARYNGRLSSVVDMKLKEG
                     NSKTYQGKLSIGTLTSSASVEGPIANRGSFFIAGRRTYMDLLLKAMAITAKLNDQQDT
                     MLLNFDANFYDLVAKANFKLSEKSHIYASTYFGQDIYGAEGRLNGDDESGWLKWDNLV
                     TAFRWNYKINNKLFVNNTLTYSNFSFDFNLNLNGSDSLKRKTSLKSDLKTGIKDCSVM
                     SDWEWSLNDKHNLQFGLGATYHDFNAGDLRWTSNFFSTIDSINVKNAAPYAKEAYIYA
                     EDTWKFSEKAGFTIGLNSAFFNVNKNIDFVLQPRISTFYLLTENLSLKSSYSRMGQFL
                     HMGS"
BASE COUNT        22430 a        13683 c        11823 g        20659 t
ORIGIN      
        1 tggtggcaac tgttgcatca aaaaacgaat ttaggtacag aatacgataa tcatttaaat
       61 attttaagaa ttgttatgaa agtgcattta tttaaaaaat aacgctttca gattaataat
      121 ctaaaaaatt atttagggaa acctttaaaa tgaaaatgaa aaaaatagat aaatacattt
      181 taccaatttg ttatactatt gtttttataa tagtaataat gatgttcaat gcccttgaat
      241 ttaactcaaa tgcaacgtac ataggtttta atgttttaac aaattcagat tcttgcttat
      301 tggttcaaat aagtaaagac atactaagtg atcccaaagc tatttttgat tggcattatg
      361 gaacctgctt ttactttttc cccaacctat tgatagtgtt actattaact acttttttat
      421 ctacaccgtc cattataatt tttgttaatt ctttattaat attctttatt acattattcc
      481 ttctcaacta tctattttta agaattttac acatttcgaa aatatgtgta gctattgcca
      541 atctaagcct aaccttattt ttagctttta cagcagactc cagcgatttt tgttttttaa
      601 ctcctcattt atttatgcca ttgcattccg gagcattaat aaatgcttta ggttctttaa
      661 ttttatgttt tatgtatcta aataataata acaaaacttc tcttgtcttt ttaattattc
      721 ttagcatagt agcaatgttc tcagatttta tttttttaat atatttttca ataccgttag
      781 tcttaacatt attgacgctt tatttttttg gaaagaaaca aataaataga aagaaatata
      841 ttactgttgt tttattactt gtattttcat atatatttac tttcatactt cacaaaatat
      901 cgattaactc agggatattt atgcttgaag ctttcaagaa tcttttttac aacataaaag
      961 attcatttat tttaatgttc aatagtattt ttgaagtatt taccaaaaat tatatatcaa
     1021 ttataatatt ctttttatct tcattttcat tagtcctatc attttttcat gccttgaagt
     1081 atttgttttt taacaagaac aaaaaaaata ttatttcaga aaattcttta ttatattcaa
     1141 tatttacatg ttatttttta gttctaatat ttgctgcaac aataataaat ggctcatttc
     1201 aaggaattga ttgcataaga tatattatac ctccttttta catcggaatt tttaatattg
     1261 gattatatat acacctatat gctcataaaa gaaataaagt agggttaacc agaataatcg
     1321 cgctatcttt tatatctgta tatacttttt ttatcatcct gaattatagt ttaataaacc
     1381 ctagttcttt atacttaaag gtaaaaaatt attatccgcc aatgattaaa gctgccgata
     1441 acatgtcatt aaaatataaa gtcaaaaatg gaattgggaa ttattgggat gcgcgtttta
     1501 ttactatatt ctcaaagcaa ggaataactg ttgatcttgt ttctccagaa tatactccta
     1561 tgaaatttgc taataatcct agaaaatatt atacaaacta taataatgac agtgtttact
     1621 acaattttat agttaattat tcaaatgaag acacagctaa attatatcag gtttttaaca
     1681 aaaaaaatat tacaaaagac actattgaag gtaatatctt ttatctatta ccttcttttt
     1741 atttagaaaa tgattttcgc acttttagga taaaagaaat taacaattag caaatatttt
     1801 aaattttcca accttaattt tttgtaaaaa atacatcaaa gaaactttga gtactcctaa
     1861 tccatatttt gaactccttc taaaatttat tgatgacgca tcatcaaagt attttgtagg
     1921 gcaggttatt tcagctatct caaaaccagc atagaatatt tgcgccaaca tttgattatc
     1981 aaagacaaaa tcgtcactat tagcttcaag attaattttt gttaatacat tcttagaaaa
     2041 ggccctatac ccagtatgat attcagacaa tttttgatgc attagcttgt tttgtgtaaa
     2101 agtcaaaaac cgattaaaga aatatttgta taatggcatc ccgcctttta ttgctccccg
     2161 tccaagaatc cttgaaccga gaactacctc ataaacatcg ttagcaatta aatacgacat
     2221 agaatgtata agctttggag tatattgata atccggatgt agcattataa caatatcact
     2281 gttcaattct aaagctcgtt tataacatga tttttggttt cctccgtatc ctttattttt
     2341 atcatgaaca actatttctt tgatgcctaa ttttctggca acttctacag tgttatcttt
     2401 gctacaatca tctaccaata taacattatc aacaatatca aaaggtattt cgttataagt
     2461 tgattctatt gttttagatg cattatacgc aggtaatact actgtaacta ttttattttt
     2521 tatcatatta aatttttttc aacaaatgta agaaaaataa taagaaaatt tacagatatg
     2581 cttccaatgt cgtttagtta aggttatatc ttcgattttt ctatcactat gtagaggctg
     2641 tgttctatgt tgtaaaacaa accactggtc aaaatagttg cttttttaaa caattaatct
     2701 aaactatcag aaatctttat gcaaataaat agaaccaaaa attgtttaaa aacttaaata
     2761 caaggataat aaatattgag ataattttga taaattagaa gttttattca atatgaaata
     2821 tatgcccttt tcaaaatatt ataagaataa agtatatttc tttttaacat taatggttat
     2881 atccattctc tcagcttctt gtgccggtta cgaccaaaca attcatactg tgtacccctt
     2941 gttactgctt atctttatat ttgttgttat catcagtatg ctggtttatg ttattatcca
     3001 gaaaatgctc atttcgaaat tgtctaacga actgaaaaac cgggacaacc ttaataaatt
     3061 aataaccgaa aatataaacg aagctgtttg taccataaac agcgagggaa taattcaaca
     3121 tgccagtcaa agctatgttt cagttatagg tttcgcccct agcgaaatta cgggcaaggc
     3181 tatttatagt tttattcacg aaaaagacaa ggaaaatgta aaacaactat ttgaaacaac
     3241 ccttcattct tcaactgaga aagtgtttga tttcaggcat ctaaaaccag atggttctta
     3301 cataattgtt gaagcttatg cccggctcct ttcttcaagc gaaatccaac acgaaggtat
     3361 tatactgagc atgcgcaatg tatccgaact gcgtatatcc gagttgcaat attatttttc
     3421 gcgctttgcg ctggacaatg tccaacagtc agtagtgatg atagaccctt cgggaagtgt
     3481 agtttacctc aacgaaaaaa cccgtcaaat actcaaaaaa aggcatataa gtgcccaaaa
     3541 cctcaaaata tgggacataa gcccagatgt agaaaaagaa aactggccgg aatattttga
     3601 gcacatgcgt agaaacaaat accgggtgat tagctctgaa ataaaaaccc ctgttggcaa
     3661 tatattcctc gaacgtcatg ccacctatac cgaatttaaa ggaaaagaat acattgttac
     3721 taattctata gatttgacaa ggtatttcaa atctgaggag ctcatcaggg ccagtgagca
     3781 acgctacaag cgtatcatag acaatatata cgatgtagtt tgggtgttag acatggagct
     3841 aaaaacaacc tttataacac cttcaataga gcggcttacg ggctatactg ttgaagaaca
     3901 catgacacag tctattgaag aaaagtttac ccccgagtcg gcaaaaacaa ttcggcaatg
     3961 gtatgcatac tacattccta aactaacttc gggagaaata aacaaagaaa cctttgtttt
     4021 acgcggcgag ctacaatata aacgaaaagt tggcgaacct atatgggtcg aaatgacagt
     4081 aaaaatacta atagatgaat caggcaaaat tattggaata catgggacat ctgttaatat
     4141 tgacgaaaga aagcaaactg agatagaact aagggcaagc cgtgaaaaat acaagtcgat
     4201 tttcgaaaac atcattgatg tttactacga aaccaccctt gatggtataa ttcttgaaat
     4261 aagcccttcg atagaaaagg tttcaaaata caaaagagaa gaactgaaag gtacctcctt
     4321 aattagcttt tataaaacac ctgaatccag ggccaacttt atagatgcta ttatgaatat
     4381 ggggtttgtt tctaattatg acctaatcct gcttgataaa gatgggcgag aaatagactg
     4441 ttctattaca gcccgtttat tgacagatga aaacgggcaa ccatgcaaaa tatgtggtac
     4501 agttcgcgac acaacagaaa aaaagcgtgt tgaaaagctc attaaagaaa gcgaagaaag
     4561 atacaaaacg ttggtttcac tctcgcccaa ccctgttata gttacacgaa acggatattt
     4621 ctattacgtc aacccttcag ccatcaggtt ttttggtttt tctgcaccag aagaactttt
     4681 gggcaggaag atgattgact ttattgaccc taaaaccaat gataaagtaa taaacgaaat
     4741 cgccaaatta tccgaatcag aaaactgccc tgcacttgag ctgggcatta ttaataaaga
     4801 aggaaaaaag ctcaccacgc tatcggcaat aaaaaccatc agttacgaag gcgaaccctc
     4861 tttattgttt ataatacaag acattacaca acaaaaaaaa tatgaagaag agctttttca
     4921 ggcaaaacaa aaagccgaag aaagcgacaa gctgaaaacc gcctttctgg ccaatatgag
     4981 ccacgaaata cgcacaccat taaatggtat aataggcttt tcaggacttt tgaaaagaca
     5041 aaaccttgaa aaagaaaaac aagaaaaata cgctgaaatt attcattcca gtagtcatca
     5101 attacttgcg attatcaacg atattattga tatttcaaaa atagaagccg gccaaatggt
     5161 ggtttaccat gagcccataa acattctgca aataattacc gaacttagct atatatacaa
     5221 aagccttgca gaaaaaaagg acattgaatt caggctcagt ccactggaaa atatccagga
     5281 aattagctca gacgaagtaa aattaaaaca aatattaacc aacctgctaa ataatgcact
     5341 gaagtttacc cataatgggt ttgtcgagtt aggctacaaa aaacaaaatc atgaagttgt
     5401 attttgggta agagatacag ggataggaat tcccgaagaa tttcaaaaaa ttatttttga
     5461 acgcttcaga caagtagaaa tagcctccaa caaaaaatat ggcggcacag ggttaggcct
     5521 gtctatttca aaagccttgg tacagatgct gggaggccgt atttggcttg attctgaagt
     5581 tgacaaaggc acaacctttt actttaccat tccttaccat accgaaaaca aacatactac
     5641 gcccaatata ccacatacgc cccagaatga aaatattaac tggagcaaca agacaatact
     5701 gattgtcgaa gatgaaagtg taaactcata ttattttgaa gaaatattaa aaaacaccgg
     5761 ggctaattta ttatttgccg acaaaggcat ggatgctgtt gaaaagtttg aaaaaataaa
     5821 aataatcgat ttggtcttat tagaccttaa gttgcccgat attgacggat ttgaagtgct
     5881 gaaacagtta aaaaaaatga acccggacat taaagtcgtt gcccaaacag cctttgcttt
     5941 gagcaacgat aaaaaagccg caatcaacgc cggttttaat ggatatatag ccaagcccgt
     6001 tgaaaaaaac gaattatttg aaacaattag taaactactt tagtttagta catttgtaaa
     6061 atgtactttt aaaaaattct cagttattct aatatagcat tgatatgaaa attttaaaaa
     6121 cctcatgttt gctcatcttg agcttaattg cattttttca acgcagcaca gcccaaaccc
     6181 gcttgctccg tttcccgaca gtgtctgaaa agagcatcgc attctcctat ggcggagaca
     6241 tttataccgt ggacattaat gggggcaatg cccgcaagct caccaaccat gtaggatatg
     6301 aatgtttccc acggttttcg cctgatggca aatggatagc ttttaccggg caatacgacg
     6361 gcaatactga ggtttactta atcccttcag aaggaggaac ccctaaacgc cttacatata
     6421 ctgccacatt gcaacgcgac gacattggcg accgcatggg acctaacaat atagtgatat
     6481 gctggacccc tgatgggaaa aatatcgttt atcgttcgcg ccagcatagt ttcaacgact
     6541 tcaaaggaaa actatacaag gtgtctatag atggagggct gtccgaagaa ctgccatttt
     6601 cagttggggg gttctgcagc tattcacctg atgggaacca attggcctac aacagagttt
     6661 tcagggaatt caggacatgg aaaagatatc gtggcggaat ggccgacgat ataaggattt
     6721 ttaattttaa aacaaaagaa accattaata ttacaaataa tgacgcacag gacattatcc
     6781 ccatgtggat agataaagat atttattaca cctctgacag aactggccgc ttaaacctat
     6841 acaaatacaa tacaagcaca aaaaataccg aacaaaaaac gctctttaca gactttgatg
     6901 taaaattccc ttcgtcaaat ggcagtttga tcgtttacga aaacgctggc tacatatata
     6961 agtacgatac aaaaacagga cagaatgaaa aggttgatat tactgtgtcg gacgataaac
     7021 tttgggcccg tgacgaaata aaaaacactt ctacgtatat caggagtttt gacctttcta
     7081 aaaatggcga aagagtagta ttttcagcgc gcggggacat atattcgtta cctgctacca
     7141 aaggcattac ctataatctc acgcgtacgc caggagtgca cgaacgaaac gtaacatggt
     7201 cgcccgatgg aaagtacatt gcataccttt cagatgctac aggcgagttt gaaatataca
     7261 tacaacctgt agatgggtca agccaggctg ttcaattgac aaaaaacgca gacacctatt
     7321 attacgagat ggtatggtcg cccgatagta agaaaatact atgggcagac agaaaattcc
     7381 gcctaaggta tatagatgta gcaacaaaag ctattacaga gataacacaa tctaaatatg
     7441 gcagtgtagg agactataaa tggtcaccag atagcagatg ggttgttttt gcccaggaag
     7501 ctgacaacca ctttagcata atcacagttt atgatacaaa aaacaaatct ttgcatgata
     7561 ttacttctaa atggtttttt tcgcacgagc caagcttttc gccagatgga aaatatatag
     7621 ctatttcgtc ggcacgcgat tttactcctt cgtatagcga tattgaatgg aactacgcct
     7681 atttcaatat ggagaagctt tatcttattt tattgaaaaa agatggaaaa tctccttttg
     7741 cccctgaaaa cattgaagtt accaccgata catccaacac agacaaaaag aaacctgcag
     7801 aaactaaaga aaaaaaatct gaaacagcag tagaaataga ctttgaaaac atagaacaaa
     7861 ggttgatacc tgttcctgta aaggcttcat catactataa tgtttggatg gcaggcgatg
     7921 gcatatacta caatgaaaga agtgaaggca aaaccaatac tttcaagtat tacgacttga
     7981 agaaaaaaga agaaaccgag ttgggcactg ccaaccgata tgccatatct tcaaacggca
     8041 aaaagatgct ggtaaacgaa agtggcaaat ggggagtaat agacctgccc acttcgaaaa
     8101 taagcctcaa ggacgaaata gattgttcta acatgatggc caatgttaat tataaaaagg
     8161 aatggcgaca gatatttgac gaagcatgga ggcaaatgcg tgatttcttt tatgttgaaa
     8221 acatgcatgg ccttgattgg aaggctgttc acgacaagta tgcccctttg gtagattttg
     8281 taggacatag agacgacctt acctacatca ttggcgaaat gataggcgaa ctcagcattg
     8341 gccatgctta catcaatagc ggcgaaaagc ccaaggctcc caggatcaat acaggtctgc
     8401 tcggggctaa gctcatcaaa gacaactcgg gctatttcaa agtaaccaaa atacttcaag
     8461 gtgccaattg gaataaagat ttgcgttcac cattgaccga gccaggagtt aaaatagctg
     8521 aaggaaacta catcctggct attgacggag ttgacataaa gcaatgtgct gatatttatg
     8581 agatgttata caataaagct gacaagacca ttgccctgac tgttaattca aaaccagaga
     8641 taaccggaag ctggcaaaca cttgttattc ctgttgcaga cgaaagcaat ctgtactact
     8701 atgaatgggt acaaaagaat ctcaagttag tagagtcggc taccaatgga gaggtgggat
     8761 atatccatat ccccgacatg gtatccgaag ggttaaacga gtttgtgaaa cacttttacc
     8821 cacaattgca aaagaaagta gttatcattg acgaccgcgg caatggcggg ggaaatgtct
     8881 cgccccagat actcgaacgc ttacaaagag tgccataccg aatgaaaatg cacagaaatg
     8941 ctaccgtgcc aggcactgta ccaaacgaaa caatgaccgg ggcaaaaata atgctgatag
     9001 accaatactc agcctcagac ggagacttat tcccttatgg tttcaagaaa ctaaaacttg
     9061 gaaaggtaat tggggtaaga acctggggag gcgttgtcgg tatatcaggc agcttaccct
     9121 tcattgacgg aacagattta cgaaaacccg aatttgccag ctattcatct gactcatctc
     9181 aatggattat agaagggcat ggtgtcgaac cagacatagt agttgaaaac gaccctgcac
     9241 gcgaatatga aggcatagat gaccagctaa acaaagctat agaactggcc aaacaattag
     9301 ccaaagaaca aaagcccctg cctgcaattc ctacagcccc tgacaaatct aaataaccca
     9361 taactagcaa aatagcactg caggattatg cacaaagaac cgcaaagctg aattatcatc
     9421 tttgcggttc tttgtattta aaaggttcca aaaattataa tattaaaagc ctttatttcg
     9481 taggcctata aataaaatat ttaccttttg tgatattttt aagctcaagc ttactcaatc
     9541 caaccttacg gcctgaaaaa ctggtatttt gatgggcaag ctcaaatacc cccggttcaa
     9601 taaccctata tataatggct gtatgatgtc ccatatcttc aatagtggta gtagtttcat
     9661 ttccattcac caccacatat ttcactttca ccttttcaaa ttgcataata tcaccgggca
     9721 aaatctcatc tttattaggg tcaagacgtt tgccatactt gtattgatga tcccactttg
     9781 catcaaccaa tattaacgcc tgattggcca agtcccagca ttctcccctg tccactttct
     9841 ttccaatatt ttcagtcaca aattcaatga tttttttgtt catttcggga agcgcaccct
     9901 gggcttttgc tgcatatcct aaaaaaaaga atgctaaaag tgctgaaata taaaaccgta
     9961 ctttcatctg tgtaaaattt aatttgttga attttaatta tttaaaccgt caatatatat
    10021 cattcaatga gagcggatag aaaacccatc ctgatttatc aggattcatg tgtgtttttg
    10081 tttaaaacaa atttaatgtt attataaaac ataaatagtt aaaaaaagtt gcagttttta
    10141 taaaccataa aatacaaata ccaaaaatcc tggatatttg accaatttca aatatataaa
    10201 tttattatat caccaatatt tatattatta aacataaaac tttatatttg taaatatttt
    10261 taagcatttt atcgtttttt ctaaatatta ttaacatgac agctttgatg ggtttgattt
    10321 ttttgctttc agcaacaatc ttttatttag taataaaaaa caaaaaacta cgcactttat
    10381 catccgaaca aaacttacag attgttttgc taaaaaaaga cttggaaaat actaatgatg
    10441 aggtaaaaaa gcttttaaaa gaaataaata aaagcgggct ttatattagg ttggtacagc
    10501 aatcgcccaa tgctattatg cttatggaca aggatgccaa tgtaatatgg acaaataaag
    10561 gttttgaaca aatgtacgaa tatacctata aagaattcat tgctgcccgt gggggcaatt
    10621 accgaaaaac gtcgtttcat cctgccgttc atgagcgcct cgacaaactg gcccgtacca
    10681 aaaaaccaat aaaatacgaa gccctgaata ttaccagaac tggcaaggag ctttggacac
    10741 aaactgcgct tatgcccatc cttggcgatg atgacgaact tattggtatg gtaaccatag
    10801 ataccgacat tcatcaacgt gttgtagaaa gtgacagcgt gatggaaaaa atggaagata
    10861 tcaacaaaaa gatggatgaa atatttgtct attttaaggc catgaaaaaa gagtcccgcg
    10921 atttgtttga aagcatcaac aattcgaaac aatggattga acaaacggac gagatagtta
    10981 aatttatcaa agaaatatcg gacaaaacca aaattctggg catcaatgct tctattgaag
    11041 cccatgtagc cggcagttat ggcgtggggt ttagggttat atccaatgag attgtagaaa
    11101 tttcgcacca aacgactaat tctgttgccg aaatcagcaa aatacttgaa aatattaaaa
    11161 aaagccacga cgggctattg caaaacaagc atgataccga aaaaacaatg gagctgtata
    11221 acgaaaaaat gaaagacctg aagaaagaga ttattgaagt ggaaaatgct atccagatgt
    11281 tcaaaacact gacttaatat ttaaccaccc ttaccttttc ttataaagga gggggaatac
    11341 aacattttaa agagtattat gagaataata gacttctaaa ctaatttgcc aatctctaat
    11401 cctccattga caatacatcc tgaattacat acgactcaaa gccccgcgaa agggcaaact
    11461 ggaataattt cttcgtaact tctttattgg cacctttaac aaattttgct tttttatcga
    11521 gctcttcttt aagaatatcg agatattcgt tctggtctat ttctttaagt gcagcatttg
    11581 caacactttc ctcaactcct ttttgccgaa gggccatgct taactttatc ctgccccatt
    11641 tattgaaccg gagcttatcg tgaacaaaag ctttggcaaa ccttttatca tcgaggtatt
    11701 tttcctggca aagtttttct atgatttgat taatgatatc ctctgaaaca tcaaaagatt
    11761 taagcttttg aaccacttca gctttgcaac gctcctgacc actacaataa cgagcagcaa
    11821 agtcataagc ttctttttct gaaaaagatt gaggagagac catatcttag aactggcgga
    11881 atttctccaa ctcaggattt tcgccttttt tgaacaactt tatctgcgcc ctgaccatac
    11941 ggggtttgcc actcaaatcg ggtttcacct gtacatttct aaaacccttc agggtaagca
    12001 tttccattat ttctttgtga aaacgcggat taatctcgaa atataagaaa cctccccatt
    12061 cgagcttaat acgggcaaag tcagcaatag ccttataaaa cttcaaaggt tcttcgtctt
    12121 ccacaaacaa agccatgtga ggttcgtaat ctaatacatt cttttgcatc tgggcagcct
    12181 cactttttgt tacatacggt gggttgctaa ctataatatc gtaagcctct ttaaaatgct
    12241 tggaactttc ggtggtaaag tcctgcaaaa tatcataatg aaaaaagttc acaatacttt
    12301 catttaaaaa cgagttttcc tcggcaattt cgagcgcctc gtctgaaata tcaataccat
    12361 cagtttgcga gtgcagcata agcttatcta aagcaatagc aagacatcca gaacctgtac
    12421 caatatcaag gatacggttt tcattgaatt tgtgcgtttt tataatccaa tcagccagtt
    12481 cttcagtctc aggcctgggg attaatacac caggggccac tttaagctta agcccaagaa
    12541 aatcagcaga acctaataaa tactgaatag gacgaaattc tttcaaccct tctgtaacct
    12601 cttgaatttt tagaaaagac tctgccgaaa cttctaaatt tggtttgagg tgaaaatcaa
    12661 ccggatgaat attggcaatt ttgtcaaaaa cgatacgcct tatgctcctg aattcatttt
    12721 caggatataa cgcacttatt tctttttcaa aatattgaag ggcttcactt agttttacag
    12781 atgatgtttc caacgctctt atttttggtt ccaatttaac aattttttac gtcaacacag
    12841 ccttttgaaa ctaaaaaaat gattttttat tttaacacac agattagctg atataaaaaa
    12901 ctatacttag ttttgttgtg tacttcaaag ttatactaca cgaaggtaga atggatacaa
    12961 aagatgagaa atatcacctt aggacacttc aattagcatt gaacgggctt ggacgtgttg
    13021 ccccaaaccc aatggtaggt tctgttattg ttttggataa taaaatcatt ggagaaggtt
    13081 atcatcaaca ctttggaggc cctcatgctg aagtaaacgc tattgcttct gtcaaagacg
    13141 aaaagctttt gcgcaaagcc acgttatacg tcaatcttga accctgtgca cactatggaa
    13201 aaacgcctcc atgtgcagaa cttatcataa aaaaacaaat ccccaatgtt gttattggcc
    13261 agttagaccc atttccggct gtttcgggca aaggactaca aatgcttatt gatgcgggca
    13321 tcaatgcagt tgttggtgag catgttgacc aatgcaggtt tctgaacagg cgctttctga
    13381 cttttcacga gaaaaaaagg ccttttttta ttttaaaatg ggcacaaacc atagatggat
    13441 tcatagatat agtacgtaat cataattctc cgcaagcacc taactggatt acagatgaac
    13501 acacccgtat attagtccac aaatggcgta gcgaagagca agccatattg attggcacta
    13561 acaccgcgct ttttgacaac ccacggctca atacccgtga cttttatggt aaaaacccaa
    13621 ttcgtctggt actggacagg cagttgaggc tgcctaagac gcttcatgtt tttgacaaaa
    13681 gcgtaaaaac ctttattttc acagaaaagc agaagaagag tgaagaaaat cttgaatata
    13741 ttcaaattga tttctctaaa cttttatatg agctgtcaac attctgctac gaaagacaaa
    13801 tacaatcagt tatagtagaa ggtggggcaa ggttgttaaa cacctttata cagcagaatt
    13861 tatgggacga agcaagggta ttcattggca atacatcatt tggcaagggt attgcagctc
    13921 cttctatgaa tagtatgcca gtatccagtg aaaatcttcc taattcgatg ttaaaaatat
    13981 ggcacaagca ccctgcagct tttaatattg ataaataagc ctatcaagta ttgaattgaa
    14041 aagagcttat ttataagaaa tctcaacaat gttgttttcc gggtcaagaa tgatgctctc
    14101 atagtacccg tcgccagtaa cacgtggttc gctggcaata taatatccgt cttttctcaa
    14161 acgctctgtt agctcctcaa gattttcttt actatccaca cctaaagcca ggtgcgcaag
    14221 cccgaaatgc tgaacattag tacttacagg gatttcggct atattaggtt tatgcatcag
    14281 ctcaatacgc gtatttccaa caaattcaat aaaatacgac tgaaacatcc tgtttttatt
    14341 gatatacaag gtactcgact tcccattgaa gtatttttcg taaaattgac gcatagtttc
    14401 gagatccctg caccagatag caacgtgagc caatgatatt ttcataaaga agtgttgatt
    14461 aggttataaa acaataggaa tacccttgta aaaatcaaga attttcaggt taaagataaa
    14521 cttatatttt gcctggagca aatcagactg aaccttcaaa taattattct gggccactac
    14581 aaaatcgatg tggcttatca agccaagctc atatttttgc ctgatatttt taaaattttc
    14641 ttcttgggca gcagcagcct tagtttgagc tttatagctt tcgtaagaag ccttagcatc
    14701 ggcaaaggct tgctccactt ccttacgaac ccctaattta ctttgttgta acgaatactc
    14761 agcatcaagt ttattcaatt tagcgttctt tacattttta acattctgaa aacgggtaaa
    14821 aataggcact gacaaattaa ttgaaagttg tttgtaaagc ttttggtcca actggtcatt
    14881 aacaggaacc ttttccccgg taagagtctt ttcatctaag ttatactggc tataatattg
    14941 cccggtaagg ctgatttgcg gactaaatgc cccttttgca acagcataac gacgttcggc
    15001 acttttcaaa gcatattcag cgccctttat ttgtggaaga gtatttaaag caacatcaat
    15061 aaccgaatcg gcattttctg caaaagtatc agggactgca agaattggag gcgctacaat
    15121 atcaaaattt ttgaccgtgt cgagatctaa catctgggct aatgtcagta atgacatgtt
    15181 cagccgattt tgggcttctg taagagaaga caaattggtc attgctgtag cttcaacttc
    15241 caattgtgca cctctggcga tacttccaac agcatacttt gcattggtat tttccaactc
    15301 taactttgaa gcctcgtatt gtttctttaa cacctgaaaa tattccttat catacaatat
    15361 ctggaaaaac gaagaggcaa tggaaaggga tatatcgttc ctggcttttt tcaggctttc
    15421 attagctgac agaaacgaat aataatcggc cctgattgtg tttaagttct gaaaaccact
    15481 aaaaatggtc agattggaac taagccccac actacccgaa ttcaggttgt tagaataagc
    15541 aaaactgcta taatcagcta cacgtccgcg accgatatta tgacttccgc cagcacccaa
    15601 atctggcaat aggttgaaat atgattgctt gtaattattt tttgaaatct gagtttgaag
    15661 ctcttgcctt ttcaaagtca ggttattttc taaagcatac gagatgcatt cttgtaaagt
    15721 catagctttt tgagagaaaa tgactgacga caaaagtaaa aaagcaatag ccatagaagc
    15781 cttaacaaca atatctttca tggtacagaa tatcgcagat taatttttat caccaaaaga
    15841 gtcaaaagta agaaatattt aatcatcctg atatgagagt ctgttttttt acaaaaaagc
    15901 gtttcaattc tggcaaactc aaatgctttt tacataagtc taaaactcaa ttttataact
    15961 caagacaggt aaaacaactg cataggtatc tttttcaatt gttttagttt taatattgta
    16021 gtaataccct ccttggttag gtgaccccag cgcattctta acctgcaatg cccatgtccc
    16081 cgagaacctg cgatggtttg tacggtaagt caatgtaaaa tcaaggtaat aagttgccgg
    16141 aaattgcttt tcaaaagcct tagcttcatc cgaaacaaca cgctgctgta caatcgactc
    16201 agcctcgttc agcggcgaat agcgctcccc tcctatataa ttgaaccgtg cattaattcc
    16261 aatcacctta tttcgtgctg taaaaaactc ttttccaacc aaaaggtttg ctgtaaagtt
    16321 tttattaaaa cgggtattgc gccaaacccc atcgtctgcc ttatactctg aattaaaaac
    16381 ggacgtagtt acaagataat aaaaattatt gttcaaaaac ctctcaaaag tcaggtcaac
    16441 gccataattg cgaccagttg agttattttc gagcttctcc ctgaaagtcc aatcctggga
    16501 gaagttaacc atagagtacg aactatcagc aattccgggg atatcataca aatattggaa
    16561 ataaggctca actttcaacc tcaacttatc ggtaatccgc cagtcataag acaaaacaat
    16621 gtgatgagcc ttggaaaagt caaggtcttt gttcagatat gccttactcc cattgatatt
    16681 ttctgaaata aaataaaact tcaattcttc aagttggcta tgttttccat aaccaaggct
    16741 tattgtctgt ttgggtgata tttcaaaact ggcgctcaac cttgggtcaa ctgaataatt
    16801 atcgttcaac aaaaaataat tggtatttat cccgccattg aaggtaaagc gagaagacac
    16861 ctcatatttt gcctgggcat aaaactctgc caatccgcta tgcccatttt ctttaacaat
    16921 attctggaat gtagaaaggg cttcattcat aatacctgat atatcaaggt tataaaatat
    16981 ctcatgataa ttaacccccg ttttcaaagt agtttgggca ttaaacttat ggttgatgta
    17041 cgaactcatt gtaactcgcc atgaattatc tttcagctta gtacttggct gcaaattcat
    17101 agatttgtcg agcatttctt ctaaaaagct attccgggtc cccgaaacag caactgtagt
    17161 actaacaaaa gactgattgc ccaggatcat tttatggttc aggccggcag cgccaatatc
    17221 caagccccag ttatacttca ctttatccca cgagctttcc cacaccatcg aatctttatc
    17281 tacaggttca ttgttttcat ctaaagcgcc aataccccag aaagaaaagg tgccggctgt
    17341 tttggtcggg aagttaaact taaacgacaa atcctgatat ttgggcactt gctgcgaggg
    17401 tatcatcccc atattagata gaatgcctgt agtagaatat ctataattaa aaaggtaaga
    17461 agctttgcct ttttcgccca aaggcccttc cgaagccaca tcaataccca tcaagcctgc
    17521 ctgaaaagta ttttcaaaac gttcattatt tccattgcgc aacttcatat caaacactcc
    17581 ggcaagcgca ttgccatact cagaagggaa tgccccggta aaaaaatcag aattggccaa
    17641 caactggctg ctaaatatcg ttacaacccc tcccccagct atatttccgc cagcaaaatg
    17701 gttagggttt ggaatttcaa ccccttcaag cctccaggaa acacccttgg gagagttgcc
    17761 cctgatagtg attgcattat cttgcacaga cccaactgtt actccggcaa aagcagatgc
    17821 catgcgggcc gggtcgtcca accctccggc ataacgacgg gtttcttcaa ccgtgaacga
    17881 acgggcgctg acagcagcca tggtattcaa cggcctgtct ttacgagtaa aggcttttac
    17941 cttcacttca tccatctggg tcacagattg ctttaaacta atattcagca ctacctcttt
    18001 ccctgacgaa acaagtattt cgggaattat aactgtctca tatcccagaa aagatacttg
    18061 tatattatac ctccctatgg gaacaccagt aattttaaac aatccatcca catcggcaat
    18121 attaccaaga cccggattgg tgtttaatac aactacactt gccccgggaa gtgcagcttg
    18181 cgattcgcta tcatatacct tcccttttac tgtttgtgtt atggtttgcg acaatgcaac
    18241 attgaaagct gcacaaataa ataatattag aagtttaatt ttcctcatgg tttgaaatat
    18301 ttgagtttaa aaagaatata atttccctgt ctccatcttg gagagaattt ttattgtatt
    18361 taaaaatgaa aaacgcgcta caattgcttt attaaggggt aattgctttg attattagac
    18421 ctaacaccac cactttcctc ttatgagtag gtcaatacat gaatttttcc acaattcgcc
    18481 ttataccaga aattaaaaga aacgaatagc taccttgcct ccaatccaga aattcagttc
    18541 ccgtccactc ggccatttgg tttttgcaat cttatatggg gcataatcgc tgaaatactt
    18601 atggttttga gattgatcag aacctacata ctgcaaatta gctgtcaagc cagcagcaat
    18661 ggatagtttg gaagccaatt tgcggtcaac gccaaaatat atcttataaa gctggttgtg
    18721 ccaatcgctg tcccatcgtt tataatactg ctgagaagaa acatccaggt cgaagctaag
    18781 cctttcgttg tattcaaagg ttgtacccaa gccataacca aatgcccaaa tcggtgcatc
    18841 taacgttgat gggtcaactc ccaaaagcag gataccgtgc agcttttttg cacctgaacg
    18901 aaaagcaagg ttcacgttat acttttcatc tgtcgaaact tcaaactgac ggatgccgtt
    18961 acgactgaga ttcactaacc caatagcaac accattcaac gtatcagcaa tattgacaac
    19021 gcccacctgc acaccgtcca cttttcgggc aacattgacc actccggcca cttgcgaacc
    19081 ttttatttca ttagccacgt tagcaactcc tgcgacctgc gtaccattag cctttccaaa
    19141 agcctggttg gtaaccccgg ccacctggca tccaaaaaga ttacgacttg tattgcttac
    19201 cccggcaact cctgcgccag tcatatcctt actcacattg gaaactccgg caacctgaac
    19261 tcccattatt gaactaacag ctacattgga aacgccggtt atctgctgca ctttaactac
    19321 agacgaatcg ccgcctttgt caaccataac attgctgacg cctgcaatct ggataccctc
    19381 aacacggcca cctgtaatgc ttgcaacacc actaacctgc gagcctacta cagattgtct
    19441 gttgaaactc accaccccgg atatttgact gccacggaca ttttttgaaa cattggacac
    19501 tccggctatc tgaacgccaa caacgttgcc gcctataaag ttgctcaccc cggctacctg
    19561 ggcaccattg acatcacttt ttacaatatt aaatacgccc ccaacttcaa aagctgcaac
    19621 agcaggaacg tatcctccaa tacaatttat tgacacgcaa actgcatcag ccccagaaac
    19681 cggattgaat gagaaatatg gtacaaaaga tatttgaata ggggatatac gctgcatttt
    19741 tgcagaatca tcttgcgaaa atgcattaaa actaaataac ccaactaaca ctaaaactaa
    19801 aaaccttttc atgactgtat tattttaaac tattaaacat tgattctttc tgaaatcaat
    19861 gctatgacag cttaaaataa atacccctcc actctgtttt gaaaaaatat cagaacctaa
    19921 taccaacgcg ggctccaata taactactca gtttatggcc tgttgaaacg ttagaagatg
    19981 ataaggtata tggaattatt tttgaaaaag ttgaactgct gctggcatca tcggtattat
    20041 aatacatgag attgtatgtt aaaccataag caagatagct gttggaagaa attttcctgt
    20101 taaaccccag ataccctctg aaagcctggg cattgttttc aacatctgac ttatggacaa
    20161 tattttgttc cataatattc agttctatat cgttcttttc atttagttgc cacatggtac
    20221 ccaaaccaat gccatagccc cacacaggat cgctgacata aaaggcattt gcgcccactg
    20281 tgaaaaatgt aaaaaacttt ttcacccctg tcctgtatgc caggttaaca ttgagcaatt
    20341 catctgcata tacctcaact ttatgataac cgttttttac atagttgaac aacccaatag
    20401 gcacgccagt gcaggtatcg gcaatgttta tcaaggcaag ctgcgaacca tccacttttt
    20461 cagcaatgtt cagcatgctt gtcatctgca ttccttcaac agtgtcagat atatttatca
    20521 atccggctgc ctgcacacct ttgttctttt ttgagatatt caaaaaacca gcaacctgcg
    20581 ccccctgaac ttctcccgaa gtgtttaaaa aaccagacac ctgggctcct ttaacttgtt
    20641 tggcatggca ggaaattcct gccacctgcg tctgaatgtc ttttgaaacc acattgatga
    20701 ggccggatat ctgcgagcct gttgtccttt ctaatatatt gatgcatccc gaaagttgcg
    20761 gcccatatac atttttacct acattgataa ctcctgacaa ctgcaaatag ctgtctttgc
    20821 tgacatttac cacaccagat atctgcgaag gagcattacc ccaagtaata ttcaatacac
    20881 ctgctacctg taccccggca tctttaacaa ctaagttacc caccccggcc aactgaaccc
    20941 ctttggccga ttcaacaata tttaaagctc ctgcagtctg acatccttgc acgtatttac
    21001 atacattgaa tactccggca gcctggtagc ccaaaacaga gtcgcctaca acattagcta
    21061 ctcctgcgca ttgtataaaa cgggcattac ctgaaacaat attcataatt ccgccaaatt
    21121 cagcaacatt cacttcctta acgtaaccaa tagtcatatt aaacgacata tcgttactga
    21181 cactcccggc caaaagttta ttggtaccca caaatggcaa caacgaaaag ctcacctttt
    21241 tgaacacctc atccttaatg ttggctgcat tggttttcag cttgtcacta agcaaccatg
    21301 cggggatatt gattttattg acaattttat tggtatcctc ctgttgtttc ttcaatggca
    21361 ttgttaccac cttattatga actacaatag gttgaatctg tcccgggtcg tagcctgatt
    21421 tgctaacaat aagttgtgca gccgaatcgc ttgccggcac caacatttta aaatacccat
    21481 attcatcagt attaactgaa acaaggctgt ttttatcata cacagtaaca tccttcaaca
    21541 acccctcatt gtcagcacca gtaatatagc cttctaccac taactttttt tgaatttgcc
    21601 cggcattctt gttttgcttg aggataatgt atttacccct tgacctgaac agcactttgt
    21661 cgcccattac taacgaaagc acgtatctga caggttgttt gtagcaattg attgttactg
    21721 gcctgatttc ttgaataaca ctactactat aagaaaatgt aacacctgct tgtactgata
    21781 ttttatcaag cacctcatca acagacatat tattgccatt gatggtaacc tgtctttcta
    21841 aaacagggat ttcggacctg actattgaaa ttgctacaaa aatgagcaat atacagagct
    21901 ttatcttcat ggcatgttgt tacaagcctc acctgtcaat aaatagcctc cggcaacttt
    21961 ttctactcgg agcccaaggg tctcagctat gatattaact atattttcta tggtttcgtt
    22021 ttcaaaactt acagtaatcc tgcaattgta tatattgccc aatgatagcg ttatatattg
    22081 accataaaca ctgttaatct gtgtcaccac atcaacaagt cgcgaattgt caaagtgaaa
    22141 gatgcgggca taagctatat ttgctttatt aacggcacgt ttgtaaaaat tatcctggct
    22201 cttaatataa aagccttttt cgccttcgtt aacgatgatt ccggggttgt attgggtgta
    22261 aaaagctacc acaccgcttt gtacatatat ttcaacagta tcgctaccct catcggcctt
    22321 gacgttaaac gaagtaccca aatcttttac aaaagctttc tctgtttgaa caataaaagg
    22381 caatgtatca tgttgtacat caaaaaatgc ttctccttta agctctaccg tcctgtgttc
    22441 tttcccaaaa ccttttttta caataatttt agcattttta tttaacctta cgattgaatt
    22501 atcaagcatc tttcgacata caacactatc aaaagcagac aatgaaagct gttcagaaat
    22561 gtcaacttta ttcgccccat aataataagc aacagtacca ataccaatta atataaccgc
    22621 aacagcagca acacgcagaa acaaattact actaaaaaac actctcttgg cctttttttt
    22681 aggttgggta tctgcggctt gcatcttttt gtgcaggctg ttccaaccct tatccacatc
    22741 aaacttctcg atacggtgtg atgcaacggt cttatcatgg gcaaaccgaa taccttcgaa
    22801 gtatttcttg ttctccccgg cctcttccat ccacctctca agctgctgca tctcgctgcg
    22861 ggtagcttct ccggtcaggt agcgggctat taaataatct atatgtttac tatcgagctt
    22921 caaaccttca gtttttattt atcaacaacc acaacagcaa tggtaaatac tctttcagcc
    22981 tttcgcgcag tatcttcaag gcttttacca tgtgcttatc caccgtttta attgatatct
    23041 caagctgttc ggcgatttcg gcataagtaa ggttttcaaa gcggctcagt acaaataccg
    23101 tgcggcattg ctgcggcatc gactcaatgg ctgcattaat ctgctgctcc agctctttac
    23161 ctataaccat ctgcgatgtc ccttcaatgg acgattctgt gttgtagcgc acatgttccc
    23221 cgtgcatttg cttaaccttg ttgtgcttga tacggttcaa acagctattg tgcacagccc
    23281 tgtataagta cgacttcacc gatgtatgga tctcaatact atcgcgctgt tcccaaagtg
    23341 ccaaaaacat attctgcacc acttcttctg cttcatccac atcatacagc atggtattgg
    23401 cgtaattgca caaacgctcg tagtagctcc tgaaaacatt ttcaaaagcc gcttcgtcgc
    23461 tattgcgtat cccagcaaca aactcactat ctgtaaccaa taccatccgc aaattttgag
    23521 gctaagatat ctattagccc tttactttcc aaacttgttt ttactcaaga caattatttt
    23581 aaacatcccc tccattcata aaaaaaacat ttttaaatac aatgctaatt ttaacattcc
    23641 atttcaaaca gatttatatt tttgtaagca cagataacac tataaagtgt atctgaaact
    23701 ttttttgatt taaaacctga acaaatgaag aactttttta aaatcttctg ccttttagca
    23761 gcattttctg tattcgctgt aagttgcgat gatagtgacg atgattcaat gtcggaactt
    23821 gacctttcta tcaattattt tatttgggaa aacatgaacc tgtattatct gtggacagac
    23881 aatgttagtg ctttggacga aaaaaactat tcatcaaaag aggatgtcga aaataattta
    23941 ctgaaaaaat atcccgacca tgaagagttt ttcaattcgt tgctttacaa aaaaggtgaa
    24001 gtggatcgct tttcgtggat tgttgacgat tatgaaaagc ttgaaaaaat gttccaggga
    24061 attaccaaat ctatgggctt tgattttgtc ctgggcaaaa ttggcactac tgataatgta
    24121 tttggccttg tacgttatgt tgtaaaagga ggcccggcag acagggcagg cctcaaacgt
    24181 ggcgactttt tcaccagaat tgacgaccag cagcttactg tctctaatta taacaccctt
    24241 ttgtcaaacg aaaactctta taaaattggt ctggcagaag tgatagacca taaaattaca
    24301 aacctttcta cttcgtacaa ccttacagcc gaagaagttc atgaaaaccc tatatacctt
    24361 gacactattt atactatcaa agataaaaaa attggatacc ttgtgtacaa cgggtttatg
    24421 tccaactatg acaaccagct aaatgctgtt tttgcaaaat tcaagcaggc tggaatacaa
    24481 gacctcatcc tcgacctgcg ttacaatggt ggcggctcat tagcttctgc caagtattta
    24541 gccagcatga tttatagcac cgacacaacc ctaattttca acaaaaatag ctacaatgcc
    24601 gacctcgaaa aatatatcat tgaaaaagaa gggaaaaacg ctttgaatga atattttgct
    24661 gcaaaaatta aagcctctga cgacactcgt gaagaagcca tcaattctct tggcttaaag
    24721 agtttatatg tcattaccac aagtagtaca gcttctgcca gcgaaatact tattaacgac
    24781 ctcaaaccct acattaaagt aactatgata ggtggcacaa cttatggaaa atacgtaggc
    24841 tccattacca tcaaagatta tgacagaaat gacaaactca accccaatca taaatgggca
    24901 ttgcagccta ttatcatgaa atcgaccaac aaaaacgggg tttctgactt ttataatgga
    24961 cttactcctg atgaaaaatt ggaagaagac atcaccaacc ttttgccctt tggcaatacc
    25021 gaagagttgt tgctaaaagc ggccattgca aaaattaatg gcacatcttc ggcaaaaaaa
    25081 gcccaagccc ctgttttcag cacttttgcc gaacgaaaag acctcatccc gcatagccgc
    25141 gaaatgcacc tgaatgatgt ttttaaaatg aaataaatgg ataaaagaga ttttattaaa
    25201 acaagtatag cttcattagg cctgttgttt attccaaaat ctgcctggcc gcttgaatat
    25261 tacccaacca cagaaaataa agaatgggct attttatata gtacatggtg cggttctgcc
    25321 agagatgcaa gtgtttggat ctcagaagga atgaatggga tagccaatgt ttttgatgta
    25381 cgcgaaaatc ctgatttgtc agagtataag tacttaattg taggtggggc tataagaaat
    25441 ggcgaaacat ctaaagaact tcaggcatat ttatcaaaac ataaagactc cttcaaaaac
    25501 aaaataaaag gcttatttgc tgtttgtggc aacatgcggc aacctgtaac aacatctcag
    25561 tacactaatt tttttgacaa tcaccttatt cccctgacaa gagccaacaa acccttgtcg
    25621 agggtatttt taggccgtat tacactaaat ctgatggaac caaaaattcg ccaacaaatg
    25681 aaatctttcc caaacatgga agattatgac aacctcaaac gcagcgaatg catggacttt
    25741 ggcagggaaa tattaaacaa cttcaatgca aatacttaaa ataaattaat tcctgattaa
    25801 catttaatat tcttctcaac ctttaaatgt aaacccctca ccattaaaat tttgaccatt
    25861 tcattgctaa atatctaact attttcccga tgattcagct tctattttat ctttagctaa
    25921 gcttataagg ctcataaaaa aatcaaaacc atcattttca attactactg tagatttgga
    25981 ttcattgtat ttgagctgaa aatacgtcat ccatttacat ctttcacgtt taaagaagca
    26041 accaacaaca gcccccgacc ggctcataaa tttcaattct ttgttattgt tactcttgtc
    26101 gatttgtgta ataatcgact ggaataattt caatgcatct gttaaagctt ctaattcgtc
    26161 tctatcgata aatgtgacca tagaaaaagg cataattggt tttacacttt gatattcaaa
    26221 ttgcaaagca aaacaaacac cccccgactt taaatctttg gcttttaaaa gccttacatc
    26281 tatctcgtct aagtttccaa gttcaataaa ttctttttca atagcaacac ctggtttgtt
    26341 aataaatatt tctatattag ttgaatatgc agcgttctcc ttactttgcg attttataac
    26401 atttgtaaaa ccaagaaata caactaacaa tatttgtatt tttttattca taaaatgaat
    26461 ttataagtta acataaagat aaacaaaaaa aggtatttgc tttttaagtt ataaaatata
    26521 acaagtacgt tattttttat gtcactgttg tccggtaaaa taaaatagat actttaatat
    26581 cgttgattag aaactggtcg ccccacaggg gcttaatttt aaagatgttt aattttatta
    26641 caaacaggac atcgctacgc gactaaataa tagcaacgta gctgcgaact gtttgtagga
    26701 taacccaata caccaaatat tcctaagcag catagcagcg acctgttttt accggacaac
    26761 aatgttttta tatgctgaaa tttaacacat acaatcacaa gatgtttaaa tacaacttta
    26821 tcttctttat aaccgcataa cggtgaaata tttgtagaaa aatgagcttt attaataatt
    26881 aggcacaaat taattgccta actatatgat ttagtagggt taaccctccc gtcatacaaa
    26941 acaagctgat ttagaaattg tttaaagcaa tccattaaat tagcgaatta caatttctta
    27001 atcgattaag tattttctga tgcttaattc ggatcaaata actatctttg aaacaaagca
    27061 taaaaaaagg cacttttaaa tttagaagtg cctttttctt ttttatggat atttataaac
    27121 ccaagtcctt tgtaatctgg gcaattttct tctcaagctc agcattggtt tctgtaaatg
    27181 ctgatttgtc tttcatagaa gcgcttacac taaagtaaaa cttaatcttt ggctctgttc
    27241 cacttggacg cacggttacc ttatctcctg cttcattgac aaattgcaat acatttgact
    27301 ttggctggtt aataacagaa gtttctccag ttattttgtt ggttgtttgt tggctttggt
    27361 aatccattac aatagcaacc tttgaaccac caaggtttgc tggtggattt tggcgaaaac
    27421 tttccatcat acgggcaatt tcttcttgtc cttcttttcc cttttttgta acatttacca
    27481 aatattcttt gaacaaaccg tattttacat atatctccat taaaatatcg taaagggttt
    27541 tacctttaga ctgtgcccat gcaaaagctt cggcaatcaa tgcgcaagcc attacagcgt
    27601 ctttgtcgcg gacaaattcg cctgccagat agccgtagct ttcttccccc ccgccaacaa
    27661 atattttttt accttcattt tgtttgatga tttcagcaat gtatttgaaa ccggtaagca
    27721 catcgaagta ttctacatta aaaccttttg ctatacgagc tataaggtct gtagtaacaa
    27781 tagttttaac tacgtattcc tttcctttta gtttgccaag ttcattccag cgggtcagta
    27841 aatagtatat caaaatagaa cctgtctggt tcccgtttaa taaaacgacc tcacccttgt
    27901 catttttaac agcaatacca acacggtctg catccgggtc tgttgccata accaactctg
    27961 ctcctgtttc tttggctttt tcaatagcca tactcattgc tgcagattct tcaggattgg
    28021 gagatttaac tgtcggaaag ttcccatctg caatattctg ttccggaatg ctaataatat
    28081 tagtaaatcc atattttttc aataccattg gcacaagggt tacaccagta ccgtgaattg
    28141 gagtatatac aattttgatt tggttattgg cggcaacagc ttctggtgac aatgataaag
    28201 ctttgatttt atcggtgtaa atttcatcaa cttcttttcc taccaattca ataagttgac
    28261 tgttgccttc ccattttacc tctgaaatgg aagttttttc tacttcttta atgatatttt
    28321 tgtcatgagg gggaactact tgtccgccat catcccaata tgccttgtaa ccattgtact
    28381 cttttggatt gtgtgaagcg gtaattacaa caccgctttg acacccaaaa tgacggattg
    28441 aaaacgataa ttcaggtgtt ggcctgaggc tttcaaataa aaacaccttg ataccattgg
    28501 cggcaaatat cctggctgta gtttcggcaa ataagcggct gttgttacgg ctgtcatggg
    28561 caacggcaac tttaattaca ggaagatcag gaaaattctt ttttagatac gtagcaagcc
    28621 cctgcgtagc tttacctacg gtgtatatat tcatgcgatt ggtcccaacg cccataattc
    28681 ctcgtaatcc gcctgtgcca aattcgagat cacgatagaa cgcgtcaatg agctcttttt
    28741 catcaccagc caacattttt tttacttcct ggcgggtagc ttcgtcatag ctgccttgca
    28801 gccattcttc agctcttttt tttactaaat ctaattcgtt attactcata tctgtggttg
    28861 tttgtaattg ctatttattc catttcttta atacaattgg caagactgtc tttccaccac
    28921 ggaatactaa tgttatagga gtttttaatt tttgatttat caaaaacact gtaagccggc
    28981 cttttggctg gcacaggata ttcgctggtc agaatgggca atactttgca attgatattg
    29041 cttatgcgta caacttcctg ggcaaaatca taccaactgc atactccttc gttagaataa
    29101 tgatatatcc ccggtttgaa gtttttttcg ttttcaatac ttagtttgag tatttcaata
    29161 atagctgtgg ccagatcatg tgcccaggta gggcttccaa cctgatcaaa aacaaatttc
    29221 aattggccta attcactgcc ttttttgcgt atggttttta caatgttgtt gccatatttg
    29281 ctatacagcc atgctgttct gattattatg gcgttttcat tgtctttgag agcaagctct
    29341 ccatcaagtt tacttcttcc gtatgatgat tgcgggtttg ttttatctgt ttcaatatat
    29401 ggggtacttt gcgtcccgtc aaatacatag tctgtagata cctgaataag tttaaccttg
    29461 ttttgagaac atacttcagc taaaaatttg acagccatgg catttatcat gttggctttt
    29521 tcttgatcag attctgcctt gtcaacagca gtatatgcag cacaattaac tacaaaatct
    29581 aaattgtgct tgttaaaata ttctgtcagg ctttctttat tacaaatatc taatgtatca
    29641 acatcagaaa agataaattc agcctgaggc aaaaaactta ctaaatcttt aatttcattg
    29701 cctaattgac cataagctcc tgtaactaat attgttttca atttgataaa attttatatt
    29761 acaattctga agaaccaaaa acaaaattca tctccgcttt ttctaaatcc ggatgaacac
    29821 ggtctttagc agaaacggca gcagttgcca tttcaacttt ccagtcaata tttagcgttt
    29881 tgtctgcaaa gtttatcccc ctttcggcct gagggttata taaacaatca catttgtaat
    29941 gtactatagc tgtttcggac aatacagaaa aaccatgggc aaaacctttt ggaacaagca
    30001 actgaagaaa gttttcatcg cttagttcaa tgccatacca atgcccaaaa gttgggctat
    30061 tcttgcgcaa atccacagct acatcaaaga ttttcccaac aacggccctc actaattttg
    30121 tttgagcata aggagccaat tggtaatgaa gccccctgat cacaccatag gttgattttg
    30181 attgattgtc ctgaataaac tcatatttta gacctttttg aagcatcttt tctttgttgt
    30241 attgttcaaa aaagtacccc ctgtcatctt taaaaactct tggttcaata actaccaaat
    30301 cttgaattgg agtaggtaaa acattcatgc tgtactattt ttttatgttc atttttttcg
    30361 agaagtattc aattgttttg agaagccctt catctaaagc tactttaggt tcccaaccgt
    30421 taagtttttc tttagctaaa gaaatatctg gtttacgttg catagggtca tcctgaggta
    30481 atggttgaaa taccaattta gattttgaac ctgtcagttt tatcactttt tcagctaatt
    30541 ccaaaatagt aaactctccc ggattaccaa tattaacagg gcctgtaaac gaatccggcg
    30601 ttacgttcat cattcgtatc atgccttcaa ccaagtcgtc aacatactgg aagctcctgg
    30661 tttgcttgcc atccccataa atggtaatgt ctttattctg taacgcctga acaatgaagt
    30721 ttgatacaac ccgcccatca ttagggtgca tacgtggccc gtaagtattg aaaattctaa
    30781 ttatcttaat cttgacatca ttttggaaat ggtaattcat aaagagagac tccgcacagc
    30841 gtttgccttc atcataacac gagcgtatcc cgatagggtt tacatggccc caataatttt
    30901 ctgtttgcgg atgcacctca ggatcaccgt aaacttcact ggtcgaggcc tgaagtattt
    30961 ttgatttagt gcgtttagct aacccacaca tgttgatagc ccccataaca gaggttttta
    31021 tcgtctttat gggattgtat tggtaatgaa caggtgatgc cgggcaagct aagttatata
    31081 tttgttcaac ctcagcataa tagggcacag taacatcgtg cctcacaagc tcaaaatacg
    31141 gattgcctaa caaatgagca acatttgatt tatctcctgt aaaaaagtta tcaaggcata
    31201 ttacatcatt accttctttt aacagccttt cgcataaatg cgagcctata aagccagctc
    31261 ctcctgaaac taatatttta ctcactacaa tgttttttta gttaaaaact ggttttcgcc
    31321 ctatactata ataagtataa cccatttcgg ccatttcaga aggttcataa atgtttctcc
    31381 catcaaaaat aagcttgttg ttcattaatt ttgcaaccat ttcaaaatta ggcaacctaa
    31441 attcgttcca ttctgtgact aatacaaggg catctgcacc aatcagggca tcgtattgat
    31501 cttttgagta tacaactttt tctccaaggt acttataagc ttcttccatg gctacagggt
    31561 catacacttt cacattagcc ccatttttca ataaattttc aataattatt aatgaaggcg
    31621 cttcacgcat atcgtcagtc tgtggtttaa acgataagcc ccagatgcca aaagttttac
    31681 ccgacacgtt gttattgaag tgcttcatta tcttttttac caggctttcc ttttgccttt
    31741 cattcacctc ttcaacagct ttaagaattt ctaaaggcca ctttttttct tcgcctgttt
    31801 ttataagggc tttaacgtcc ttaggaaaac acgatcctcc ataaccagca ccagcataaa
    31861 taaacttact tcctatgcga gggtcgctgc caataccctt tcttacccag gtaatatcgg
    31921 ccccgactat ttcgcacaag tttgcaatat cgttcatgaa actaattttg gtagcaagca
    31981 tggcattggc cgcatacttg gtcatttcgg ccgaagcaat atccataaac agtataggat
    32041 ggttgttaag taaaaatggc ttataaagct tacgcattat tttttcagca tcttgagatt
    32101 caacgcctac tataatcctg tctggtttca aaaaatcttc aatagcattt ccttctttca
    32161 aaaactcggg attggaggcc acatcaaatt tgacagatac cccccttgcg tccagtgagt
    32221 cttgtacagc cttttttacc tttagtgagg taccaatcgg cacagtactt ttcgttatta
    32281 cgactaagta atttttcatg tacttaccaa tgtctgaagc tacggccaac acataccgaa
    32341 gatcagcact gccatcttca tcaggaggag taccaaccgc aataaacact gcttcacagt
    32401 ctgcaatagc atcagctaag ctggttgtaa aacgcagcct tcccttggcc atgttccgtt
    32461 ctatcatttg gtcaaggcca ggttcgtata ttggagtctg tccgttattg agtttttcta
    32521 ttttttgagc atcaacatca acgcacgtta catcaagacc agtttcggca aaacatgtac
    32581 ctgatactaa gcctacatat ccagtaccta caatagcaat tttcattata aaaaattttt
    32641 ttttaaagcg tgtaaagtta gcagtttttt aaaaaatatt agaccgaaaa aatatcggat
    32701 attagcttta ttgaaaatat ttcacctgcc cacatttcat tatggaaaac tttgaacatc
    32761 ataattttat tttttgtatc tttaactcaa ttaagaataa agttttactt ttgcaactct
    32821 catttaatat tacatttaat attacataat gtctaactta ttaattttta agaaatttca
    32881 atggctaaaa aagaattaga aaaagaagaa ttcaacgaag aattgttgag caatgagatt
    32941 gttgacaatg aagaaattgt tgacgacgta gaagaagtag aagaagactt tgacgaagtg
    33001 gacgatgata gtttaaaaac tattgtagaa ctgaaaaaag ctcaacatca aaaagaaacc
    33061 gctgaatttg actggaacaa ttacaccaac aaaggcgaaa aatatgctgc tgatgtaaaa
    33121 gcacaatttg aagctatgta tgacaaaacc ctgtcaacta ttgcagaaaa tgaagtagtt
    33181 gaaggtacag ttatttcaat gaacaaacgt gaagtagttg taaatattgg atataaatca
    33241 gaaggcgttg ttagtttgaa cgaattcaga tataatccgg accttgcaat tggcgataaa
    33301 gtagaagttt atgttgaaag tcaggaagac aaaaaaggac agcttctttt atcacacaaa
    33361 aaagcacgtg cattacgttc atgggaccgt gtaaacgaag cgcttgaaaa agacgaaatc
    33421 atcaaaggtt acatcaaatg ccgtactaaa ggcggtatga ttgtggatgt atttggcatc
    33481 gaagcattct tgccaggttc acaaatagac gttaagccta tccgcgacta tgatgcattt
    33541 gttaacaaaa caatggagtt caaagttgtt aaaatcaacc acgaatacaa aaacgttgtt
    33601 gtgtcgcaca aagcacttat tgaagcagaa cttgaacaac agaaaaagga tatcatttct
    33661 aaacttgaaa aaggtcaaat ccttgaaggt acagttaaaa acattacctc ttatggagta
    33721 tttattgacc ttggaggtgt tgacggactt atccacatca cagacctttc atggggccgc
    33781 gtaaatcatc ctgaagaaat cgttcaactt gaccaaaagc tcaatgttgt tatccttgac
    33841 ttcgatgatg acaaaaaacg cattgccctt ggcttgaaac agcttacttc acatccttgg
    33901 gactcactta gtgccgacct taaagttgga gacaaagtaa gcggcaaagt tgttgttatg
    33961 gctgattatg gagctttcat tgagatagcc ccgggagttg aaggccttat acacgtttca
    34021 gaaatgtcat ggtcacaaca cctgcgtagt gcgcaagagt tcctcaaagt tggcgatact
    34081 gttgaagcag ttatccttac attagaccgt gaagaaagaa agatgtctct tggtatcaaa
    34141 caattgaagg cagatccttg ggaaaaaatt gaagaaaaat atgcaattaa ttcaaaacat
    34201 aaagcaaaag ttcgtaattt tactaacttt ggtgtgtttg tagaaattga agaaggagtt
    34261 gatgggttaa tacacatttc agacctgtca tggactaaaa aaattaagca ccctgcagaa
    34321 tttacttcta tcggtgaaga aattgaagtg gtagtattag acatagacaa agaaaacagg
    34381 cgcttaagct taggccataa acagttggaa gaaaacccct gggatgtatt tgaaaccatt
    34441 tttactccag attctataca tgaagggacg atagtagaga tctttgataa aggcgcaatg
    34501 attgcattgc cttatggtgt tgaaggattt gcaactccaa aacatcttgt aaaagaagat
    34561 ggtgtgatgg ctaaagtaga tgaaaaactt gaatttaaag ttattgaatt caacaaaaca
    34621 gctaaacgta tcatcttatc gcatagcaag gtatatgaag atgcaaaaaa agctgaagaa
    34681 acagctgcta aaaaagccga agagcctgca acacgtaaag ctggtaagaa agtaaaagct
    34741 aacattgaaa aaacaaccct tggtgatatt actgagcttg ctgccttaaa aactgaaatg
    34801 gaagaaaatc agaaaaaagg gaaacaagct gaataagtaa cattcaacaa attctaaaat
    34861 tgaagacaat ggaatttaag attgaaaagc tgcagaatta cacaattatt caagtgcttg
    34921 aagagaaact tgatactcat attgcgccat cattgaagtc tgagcttgtt ttgatttcag
    34981 gcaatggcga aaaaaatatc attctcgatt tgagcaattg caaatattgt gattcgtccg
    35041 gattaagtgc aatccttgtt gcaaaccgtt tgtgcaaaaa tgccggaggt aaatttgtac
    35101 ttacaggttt aaatgatgcc gtagaacgcc ttatcattat ttcgcaattg gatacagtgc
    35161 ttaatattac agaaacaatc aacgaagccg tttctttaat ttcagacaat aaagccaatt
    35221 aatggttttt gaatgtcact ttcagttacc atattaggta gtagctcggc tattcctacg
    35281 tcgaaaagaa atttaaccgc tcatctgcta aaccacgatg agcggttttt tttgattgat
    35341 tgtggagaag gcacccaaat gcagttacag cgcttcagaa ccaaatacct tcgtataaac
    35401 cacattttta ttagccacct ccacggtgac cacatatttg gcctttttgg gctattatct
    35461 actttttcca tgatgggcag gaaaaacaca ctgcacatat actcacctgg cagattaaaa
    35521 gatttgctaa attctcattt tgcgtttatg ggcgaaaccc tcaattacca aattgaatac
    35581 catgaagtgg atgcgaagat gcccgaagtt atatatgacg ataaaaaaat tactgttact
    35641 actatccctt tgcggcatcg tatcccttgc acaggatatt gttttcggga acatcctaaa
    35701 cttttaaaca tcaaaaaaga atgtattgag aaatatcaac tgggagtaaa agatattgtg
    35761 gatattaagc aaggagctga ttttgttctt gaaaatggga tcgtcatccc taataaagag
    35821 cttactacag aaccagtccc gccaaagtct tatgcttttt gttcagatac aaaggctttg
    35881 cagcgcattg taccttatat tgccaatgtc gatttgcttt accacgaaac aacatttttg
    35941 aatgaagatg caaagctggc taaagctact tatcatacta caactggcca agctgcgcaa
    36001 ttagcattgg aagctaatgt tggaaaactt gtcattggtc acctttcatc aagatacaaa
    36061 gatgataacc gctttatcaa agaggtgcag gatatttttc ctaatgctat catggctgaa
    36121 gatgggatga aaattgatat ctaaaacatt caaatataag atacaaacac ccatgcccca
    36181 cccgatttgt cgggacaagt tttcccgctt ttacagaata agctatctgt ccattaaaaa
    36241 tcagttttat acaaatcaat aataaaattt attcaacttt caaggagctt attcaacaat
    36301 gttacacttt agaagctatt tgaaaaccaa tgtataaaca tatctcaagc ataatatgaa
    36361 tatctaaata caatgctttt ttcttttcat acaaatcaat taactttggc acatgaattc
    36421 gtttgaaatg gaagaaagta atgacaaaca gcttttgctg gcccaaaaat tcagctcttt
    36481 tgtaaacagc cacaagagct tctactttac tgaagatgaa tacgaagaca tcattgaata
    36541 cttcaaactt cacaacaaca aagaacttta ttacaaatcc attatagcgg caaacaacca
    36601 atacccttac aatcaagaat ttaaatattt ccaaatagaa tattttcttg aaaataaaca
    36661 atattctgaa gcacaagaat acctgcaaaa aagtctcggt tgtttgtttg atgaaaaaaa
    36721 caagatctat taccaggctg caattttcta ttgtaccggg caccaagata atgcagaagc
    36781 actgataaaa agcacaggca ttgaaggcat tgacgacttt ggcgaatttt ataccgagct
    36841 tggtgagctg tttatggaca tcaaacgata cgatttggcc attaaggctt tttccagcgc
    36901 ttatctcgaa aatcatgaat acgaaagact ttgcgacatt tttgatgcca gctttgaaga
    36961 tatcgaaaca ttcaaagaag ccaacagctt tgattttttg accgaaaagc tcccttttga
    37021 tgccaacgta tggttaaaaa aatacgaata ttgtttatcc atctttgatt acgaaaaagc
    37081 tttagaagct ttagattttt cgttagccct cagcccggac gaatatctcg aactgaaaaa
    37141 agtagccctg cttgtttacc tcgaaagata ctccgaagca ataaaacttt ttgagcaact
    37201 caaccatgac attcgagatg aagatgaaac attatactgc gacgagtttt acgttgtttc
    37261 ttttgcctat ttcaagctca aacaatttga aaaagcagag tattatgcca tggaagacct
    37321 tcgtctcaac aagcttgaaa gcaatgctac cgccattctc ctttctgaca tttatgtaaa
    37381 aacaggcaaa agggcaaaag caaaaaacat actgtttgat ttgctaaaaa aagaagaaga
    37441 aaatattgac attcttttca ggctttctga aatatatgaa attgaaggca atatgaacaa
    37501 agccattgag atgatgctaa agcttttaaa aaacgattca tcagatgtta ataatttttt
    37561 gagactggca aacctttatt tccagataaa ccttctttca aaagccatca gaacacttga
    37621 agaatcgagc aatattgaac ctaccaatgc taatattcat tattctctgg cagcttatta
    37681 tttgcttcaa aataaaaagg acttatctgt caagcattta aactatgctt taaacctaag
    37741 cccttctaaa tttcatactt tgctggatat attccctgaa gcgcttcaaa tagaagaaat
    37801 ccgaacttgt tttcacaaaa acaaccttag aatatgaatt ttccgttaac taatatccct
    37861 ttaagggaaa gcaagccccg caacaacggt attactatga taatggacaa agggcttagc
    37921 ttaagagaag ccgaaaacct tatatcatct tgtggcagtt atgttgacct ggtaaaattt
    37981 ggctttggca caggccttat tacgatgaac cttgaagaaa aaattaagct tttctctgaa
    38041 gccaacatca aaccatactt tgggggtact ctttttgaaa tatttcttgt acgagggctt
    38101 tttgatgatt tcagaaagtt cattgacaaa tacaaagtcg aaatgattga aatttctgat
    38161 ggttctatct ctttaccaca cgaagaaaag cttaaataca tcaatattct ttcaaaacaa
    38221 gccacagtag tttctgaagt aggttcaaag gtggccgggg ttgttatttc gcccgaaaaa
    38281 tgggttgaaa tgatgaaaca tgaacttcag gccggttcgt ggaaagtgat agccgaagcc
    38341 cgcgaaagtg ggaatatagg gatatacaac ccagacggct ctgctaataa agaactcatc
    38401 aatgacattg taaaacatgt caacaccgac aacattattt gggaagcccc caacaaacct
    38461 caacaggtat ggttcattaa gctattaggg gccaatgtaa acgttgggaa tattgccccc
    38521 aatgaagtta tctcgctcga gaccttacgt ttgggtttgc gcggagatac cttttttcag
    38581 tatttgcctg aaaatgttgt aaaaaaatac tttgtaaaat agcatataca tgaagcaatt
    38641 tggtttaata ggatacccgt taggacactc cttttcaaaa ggttactttg ccgaaaaatt
    38701 taaaaaagaa aacataacag attgtattta tgacaattac cctattgaaa atattgggct
    38761 attcaaagac ataattaata aaaacaccaa tattgttggg cttaacgtta ctatacctta
    38821 caaagaacag gtgataccat ttctaaacga gatagatatt gaggctaaag aaataggagc
    38881 tgttaatact gttaaagtga aaagaaatgg caatactgtc attctaaaag gcttcaatac
    38941 tgatgtttat ggatttgaaa ccagtttaaa acctatgtta gaaaagcaac accaaaaggc
    39001 gcttatctta ggaacaggcg gggcatcaaa agccatcaag tttattttga gaaaacttgg
    39061 tatttcttat atttcagctt ctattgaaga attaaaagaa aatgaaatca ggtattctga
    39121 cattacgcca aagttaatat ctgaacatct gcttattatc aatgccactc cgttggggac
    39181 atttcctaaa gttgacactt gcccagacat cccatacaac gcaataacat cgcagcacgt
    39241 attatttgac ttagtttaca atccggatgt aactcttttc ctaaaaaaag gaagggacaa
    39301 tggggctaaa accaagaatg gcttacagat gttgcacctt caggccgaaa gagcctggca
    39361 gatatggaat agcccggagt tttaaaagtt atgcatcaat ttccatggca tcatcagcgc
    39421 aggtttaact cttatgctga gtattttaaa aggcattttg ggcatagggt ccagaaactt
    39481 accattgatg ccgggtttac ctgtcctaac cgtgacggga gcatagctac cggagggtgt
    39541 acatactgca acaacgaagc ttttaaccca tcgtattgta ctccacacaa atcaataacc
    39601 cgtcaattat ctgaggggat agagtttcat caggtaagat atcgcaatgc acaaaagtat
    39661 ctggcctatt ttcaggcatt ttccaacact tatgcacctt tagaaatttt aaagcagtat
    39721 tatgaagaag ctctggcatt tccggacgtg ataggcttgg taataggcac gcgcccagac
    39781 tgtgtagatg ctgaaaagct tgactatatt gcccggcttg cccaaaggta ttacatcatc
    39841 atcgaatatg gcattgagtc tatctataat actaccttaa aaaaaattaa ccgtgggcac
    39901 agctttgaac aagctgttaa agcaattgaa gagacgaaga agcggaatat caaaacgggg
    39961 gcgcacttta tcattggtct ccctggtgaa accgagcaaa tgcttttcga ggcaatcccg
    40021 gttatatcaa gcttaccctt agattctatt aaatttcacc agttgcaaat ctttaaaggt
    40081 acagttatgg cttctgaata tgcagcaaat ccaaaggctt ttaatcttta tagccttgaa
    40141 caatacctcg agcttattgt tagggtagtt gaacatttaa accctgctat tgtcattgag
    40201 cgtattgcag gcgaagcgcc tccatggcat ctggcaagca cttcgtgggg cctgcttcgt
    40261 ataaacgaca ttgttaggct ttttgaacaa aagcttgaag aaaaagatac ctggcaaggg
    40321 aagttttacc tataatcaaa ttttttccac attgctggca agcatccaac cgttgttacc
    40381 atcggcaatt ttaatcctga gccattctcc cacctggtca ataatgataa ctttagtccc
    40441 ctcgtgtata acaaaaagct ctgtactttc tttatcaggc gagctcctaa cggtaaccgt
    40501 ttcgctcatt acaatagctt gttgttgtcc ttcaataagc cgttttgtat gcaagctatg
    40561 actaaatgca attcccgaaa ataaaaacag gataatacca catgtaaaag ctgttttttt
    40621 tacaaaaagg atattgacaa ataaatataa caatacacaa aacaatgata tacaaaatgt
    40681 tactatactc catatagccc aggcatctgt agaggccagc tcagaaaaat tgcgccacca
    40741 acgactgaca aaaaactcgg gcaaagtgtt aatcttgtca acagccaaag cctgtgccaa
    40801 ctggagatta aaagtaatat cctcatcaga gggtgacaat ttatgcgccc tttcataata
    40861 aagaatagca tgggcaatat cttttttctt ataaaaaaca ttgcccatgt tataaaacaa
    40921 ttcagaagat ttatacccca ggctgtctat agctttgtaa aaaattaaag cggtatcata
    40981 tttacctttt ttataagctt cgtttccttt gttaaaaagc gcctcattgc tttctgcagc
    41041 cattgcagca atttgtaaaa taaaagctaa aagcagggtt aaaacattat tcttcatatc
    41101 ttgatttatc ttttttttaa ctaaagaagt tacttataat gttgttcaac ctttgtgatc
    41161 acatcaacgg catcatcaaa tacctgtttc atttcggccg agccgccggt aggggcatac
    41221 ctggccattt cgcagcggtc aacaactgaa ataaacttaa ccaccacctg ttcgtcaagc
    41281 tgtaaattct gaacatattc agttacagta tcccgcgaaa gtttagctac cggaattccc
    41341 aatttgtcgc ttacatatcc ccacatggct tttaggacaa gttcgtaaaa ctgttcaccg
    41401 ttattgtcat gtagcgatgt tttagcttct ttcaatagtt tttgggccat tttatttgct
    41461 ttcttattac gaacaagcct tatgttggca ttttccttaa tcttcttacg cccaataatt
    41521 actgcaacaa taaaaagaag aagaggcaac agataaccca atataaacaa agctgagcca
    41581 aaaatatact ctcccttttt cctgaatgac ggggtttgcg ttttaataaa ctgtatatct
    41641 ttccctataa acttaatatc ttgtttgtcg attccagaaa caacctcaac ggtattgtcg
    41701 ttagcgcctt tctcaacctt aatattaata tctcctgaag aaagggtttt gtattgtttc
    41761 agtttagtat cataataaga gaagctaaac gaaggaatgg tatattcgcc tgcatgacga
    41821 gggataataa ggtattcaaa cgttttagaa cctttgaccc cggcagtagt aacctgcgaa
    41881 cttacggtaa cctttgggtc gtatgtttca aagtcagcag gaaacttgat gtcgcaaggt
    41941 tcaataagct ttatattacc actacctgct attactactt ttaaattgat agcatcatta
    42001 gtttttacag tatttttgtt aacagtggca ctaaaagtat agcttccgcc aatatttttg
    42061 aaatcggcag gagctcctgc aggaaatggg gaaaccgaaa ttttaacagg gttgcttttt
    42121 actttttttc taacttctcg aacctgaggg ccaaaaaagt cgtcccaaac actgcgagaa
    42181 cggttacgca tgactatctg caatacagcc tgaagagaaa atgggtctat cactatttct
    42241 ccatttttct gagggaataa taccattttt tgcagtaccc cggtataata aacctgtcca
    42301 tttaccactt ctttttcaag ttttgaaagc tggggtgttt caatgtcttg tctgtaaaaa
    42361 cctccaaaag atgggtactc tacattttcg atagaggata tactatgctt agagaaaagt
    42421 ttgattgtag ctacaatatg gtctccacgc gaaatactgt ttctatcaac aataatacgt
    42481 acaaaaaaat cgtcacttcc actatcatca acatctgata aagactgttg agattgttgt
    42541 gtttgatttt gttgttgatt ggaggcattt gattgacgtg tagtattgtt ttttccggca
    42601 acttcgattg ttatagaatt tgacatatac gatttaccat ctacagtagc ctttgccgga
    42661 tcgaaagtat atgtacccgg gttgttagcg ctaaaatagt aagtatagct aaaagtagta
    42721 ctttgagtaa ccttgccatt aatgaattgg acactactac ttgaggaagt gctgggccct
    42781 ccaagtaaat ttaagccttt aaattcagga ggcctgaaac cacttggctg ggcattaagc
    42841 gtaaaagtaa tttcaaattg ctcacccact tcaacaaccc tgggggcaga agctacaaat
    42901 tggatatttt cggccataat actcaatgac aaaatcaaaa gaagtgccaa caggctcata
    42961 cgcttcttta ttaaaccatc gaatgcaatg ggaattaccg atttcatatt aacagaaata
    43021 tttttaaaaa tatttatcat agtattgaga gtttaccaat ctttttcagg gtttgtcttc
    43081 tgacctttag ctttttcttt ttttactttt tcctgggtgt tattttcgtc atcttgtata
    43141 gcacggagaa gttgctgagc ctgttctttg ctcatttgcc cctgttgctg ctgaggttgt
    43201 tgttgctgtt gttcttgttt attctgatcc tgattgttat tttgaggttg attttgttgt
    43261 ccctgattat ttttatcttg ttggttttgt tgattttgct tattttgagg tttatctttc
    43321 aataattttt gggcaacagc cagattatac ttagactcca tgtcattggg gttattccga
    43381 agcgcttttt tataagcatc aatactttcc tgaaactttt gcatttgcag atatgtattt
    43441 cctaaattat ggtatgcttt agcaatatct tttttatcaa ttttggcatc agccgcatga
    43501 gtataagcat taacagcttc gtcaaatttg ttttgcctgt ataaggcatt ccctaagtta
    43561 taggtggccg ttgggatgta cgaaccttgt tcttctaaag ctttacgaaa gtcaacttca
    43621 gcttcattaa attttttgtt agtatattct ttgttgccct tccgaacgag ctttttatca
    43681 ttctgagcga gagcatgtac taccaaaaac agcgatatga atataaaaat taacttcatc
    43741 atgctatctc ctgatttttt taacttcaaa taacttaaaa ttaacgagca tcttactttt
    43801 cttttcaatc aataagaatt caattatcaa aagaataatt gccatggcga agaaatactg
    43861 aaattgatcg tcatattcag aatatatttg agcatggtat tcttttttat tgagtttgtc
    43921 aaccctgtca aaaagcttat tgataccagc ctgtgtattg ttgccacgga tataatcccc
    43981 gcctccggca gcggcaactt gggccaaggc ctcctcattc agtttggaga tgatggtatt
    44041 tccttctctg tcttttttaa aatttaaaga actagggcca ccttcgggaa ttggggctcc
    44101 ttcaggcaat cccatcccaa tggtataaac aagcattcct ttttggttgg caattttcgc
    44161 ttcttcaatg gcatcatctt catggttctc accatctgaa attactaata tcaccttttg
    44221 gagctcacta ttaggcgaga acgaattctc agccatgcgg atagctttac ctattgcagt
    44281 accctgcaca ttgaccattc cagtattaat atggtttagg aacattttgg ctgaaacgta
    44341 atctgtagta atggggattt gggtataagc ctcacctgca aatactatta aaccaatctt
    44401 atcgtcagtc aatctgtctg taattttact aatgacattt tttgctttct ccagtcggct
    44461 tggttcaata tctgcagcat tcatactgtt tgatacgtcc aaagctatca ttatttcaat
    44521 acctttacgt tttacttcct gtaatttgct tccaaactga ggccttgcaa gccccaaaat
    44581 aacaaaaacc acagcagcta aaaacaaata aaacttccaa attggcttag ttttggaaaa
    44641 ttcaggcatg agctgttcta caagctcact ttcgccaaaa cgggcaagcg ctttgctccg
    44701 ctggatacgt atccagtgaa aaaccccaat caatatgggg attaacaaaa gcacataaag
    44761 catatatgta ttttcgaacc taaacataat ccatcatttt aaggtataac acgcaaaact
    44821 gtatgtttca gggcaaacga agctaacagc aataacaaag cagcaatgcc aaaaggcata
    44881 aaaacatcat cacgtttgtg atactcttta acctcaacac gggttttctc aagtttatca
    44941 atctctttat aaatttcacg aagcttttgg ttgttagttg ccctaaaata cttagccccg
    45001 gtcatttgcg caatttgttt gagcatgggt tcatcaatat tgacctccat atcctgatac
    45061 tgagttccaa aaggggtttg cacaggataa ggggccatgc caattgtccc aacgccaata
    45121 gtataaacgt gtatcccgaa agctttagct atttcagcag cagttacagg ggcaatagac
    45181 ccctggttat tcaccccgtc agtaagcaag atgattactt taccagcttt tttggcatcc
    45241 ttcaaacggt tcacggcagt tgccaatcct gaaccaatgg ccgtaccatc ttcaatcatg
    45301 cccattttta catcatgaaa gaggttgata agtacagaat ggtcagtagt taaagggcat
    45361 tgggtaaaac tttctgccga gaacactact aatccaatac ggtcatctgg tcgtccgctg
    45421 ataaactcaa ttgctacttc ctttgcagct tccagacggt ttggtttaaa gtcagcggca
    45481 agcatactac ccgaaatatc gagagccatc ataatgtcaa ttccttcaga cgaaacgttc
    45541 ttccattggt tggttgattg aggccttgca attgcaacaa caagcaaagc aaatgctaac
    45601 atattggcag caaaaacaac atgcctaagc tttgcacgca ttgatttttt gacatttgcc
    45661 aatccctgaa ggtttgaaaa gaccacagag gcagacatct tttgagcttt tcggacatac
    45721 cagattacag ccgggataat cagcaaaagc agaaaaaaca ttttaggatt tgcaaaaact
    45781 atattaccca taattcaatg tcaatcaaat aaaactttac tttacatctt taccctcagg
    45841 ctcttctttt ttttcattaa ctgatggttc agtagcaaca actggtatgg tattcttaac
    45901 gaaatcatac gcaacagccc aaatattttc attttcatta ggagctggtt ccattttagc
    45961 aaactttaca aggtctgcca acgaaaacat ttctgtcaat tttgtaatta aagatttttc
    46021 tataacatca atagttttca accttgaaat aatttcttcg cttgtacttt ccatggcagg
    46081 tacttcaaaa cgggcttcaa tataagttct tacagcatca gtaatacccg aataatactc
    46141 tttaataaaa ccacgctgcc ataacttttc gcgttgtatc ctttcaagct ccctaagagc
    46201 aataacatgt gcaggttctg ccggtttggc aaatatcccc aatatgggtt tatgggtttt
    46261 ccaacgccag attacgtaag ctattaacag aatggccaat aaaattgcca gccctatacc
    46321 tgtataagcc agaatttctt tcaaagtaag aggaatttca attacgcctt ttatatctgc
    46381 aattttcgaa gggtctttta cctgaagagt atttactgcc aacataattg gggcacttac
    46441 caaagtatct gttgttgtgc cattgaaaaa agcaaacttc aacggttgag tctgataaag
    46501 cccgctgtca aaacatgtta caagatattt ttttgtaacc ttaatattgt tggcatcaat
    46561 gtgagttgta tcggcaggta aagaagacaa aacctcaatt ttagatacaa tagagtctgt
    46621 aaatactgga aacattacct ttacgccaac aggttgtgta actaccatgt taaggtacat
    46681 ttggtcgcca ataagaatct gggccgaatc aaaacgggca tcaacgctta ttgttcctcc
    46741 tgcaaatatt tttgcagata ataaaattag tacaattgaa aataatattt ttttatgtgg
    46801 ctgcattttc aagcaattca tattaatttt acaatgatta gcgcatctca aaaagcttca
    46861 tcaacggctt cacgtaatct tctccggtat ataactgtac tgtgtcaatt ccacacttag
    46921 aaaacaagtt ttgcaagcgt tcctggcgtt tcatccacca cagggcatat tgctgcctga
    46981 cctcagggtc tgaagtgtct atccatgttt ctacatcaga ttcagcatca acaaacttca
    47041 tcaaacctac tggtacaagc tctgcctcac gacggtcaac cacctgcaaa gcagctatct
    47101 cgtgtttacg gtttgctatt ttcaaagctt ctgaaaaatt gataacccca tcatttgttt
    47161 cgtcaaaaaa gtcagaaatg acaaacgctg tacatttttt cttgatagaa ttggtaaaat
    47221 aacgtaatgc ttccgaaata tcagtttttc ggctcttagg ctcgtaagtc agcaactcac
    47281 ggatgatgcg caaagtatgc gaacggcctt tttgcggaga gataaatttt tctactttat
    47341 cagaaaacat aatcatcccg atcttatcgt tattttgaat agccgaaaag gctaacacag
    47401 ctgcaacttc agcaataagc gaagttttaa gctgcttgtt tgtaccaaaa tgacgcgaac
    47461 cgctcacgtc aacgagcagc attacagtaa gctcgcgctc ttcttcaaaa actttaacaa
    47521 aaggatgatt aaaacgggca gttacgttcc agtcgatatt acgaatgtcg tcaccaaact
    47581 ggtattcacg cacctcagag aatgccatac ctttgccttt aaaggcgcta tgatattctc
    47641 ccgcaaaaat atttttagtc agcctacgcg acttaatttc tattcgtcgt actttcttta
    47701 ataaatcggc agcttccaca attacctgat tttacttttt ctttttcatc aaaatggtga
    47761 aataccattc atcttttatc agttcgatga tgtaaactgt acctttttct acataaatcc
    47821 acttagtatc gcctgctttt ttgtacattt tgtccaactc ggcggtgcgc aacttcatat
    47881 tagagtagtc gctcatcagt ttggtaaatg tacattcgtc cttttcatcg aggaaaaaca
    47941 ggtacgtttc ttcattatac ttatcaacga actttatata cttataggtg ttattgacag
    48001 caccttcatc gaggtcaaag cccgggttgt ttttttgcat caggctgata atctcggtct
    48061 ttgtttttcc tatataatta acaggttcaa cttgtacgac caacgatata aggcctaaaa
    48121 gcattgccaa tgttttcata gctaaaattt taaaaactcg aaattaaaca attatttgtc
    48181 aaactccgaa tttttacagc ccaagttcac cctctactat ccgggagctg caatacttca
    48241 gtctaaggga cctcaacggt attgagaatt tcagtgataa catcttctgt agtgatgttt
    48301 tcagcctctg cttcgtaggt aagaccaata cgatgacgca atacatcgtg gcataccgca
    48361 cgaacatctt ccgggattac gtatccacga cgtttgataa atgcataagc cttagaagcc
    48421 atagccatat tgatacttgc acgaggagaa ccaccgtaag ctatcatatt ctgaaatctc
    48481 tcgagcttat attcagatgg gaaacgtgtt gcaaatacaa tatcgaggat gtatttctca
    48541 attttctcat ccatgtaaac atcgcgaacc acagttttag ccttaataat atcttcaggc
    48601 ttcaatacct gtgtggcttt tggaaatgtt tgccccatgt tttggcgaac aaccaagcgc
    48661 tcttcttctt ttttaggata agtaatcacc accttgagca tgaaacggtc catctgcgct
    48721 tctggcaaag gataagtacc ttcttgttca attgggtttt gtgttgcaag aactaagaac
    48781 ggttgtggca acttaaaagt ctcagcgcca atagtcacct gcttctcctg cattgcctcg
    48841 agcaaagcac tttgtacctt agccggagaa cggttaattt cgtctgccaa cacaaaattt
    48901 gcaaaaacag ggccgcggcg taccgtgaat tcctcgttct tttgactgta aatcatagta
    48961 ccgataaggt cggcaggcaa gagatccggt gtaaactgga tacggctaaa cttagcatca
    49021 atagcctgag caagagtatt gattgctaat gtttttgcca gaccaggcac accctccagc
    49081 agaatatgcc catcagacag aaggccaatg agcaaacttt cgaccaagtg tttttgacca
    49141 acaataactt tgttgatttc cattgacaaa atgtcaacaa aagcactttc ctgttgaatg
    49201 cggtcattta attctttaac attaactgca atattcatcg atctggtttt ttgttctttt
    49261 tacaatataa aagtgaacca tttcagtatt gttaaaaagc taaatagttt tataaatagt
    49321 taaaaagtgt taagctttga acgttaggat aataaaattg agaatgagag aatgagagaa
    49381 tttgaaaatt tgaaaatgcg agaaatgcaa aagattgttt gtttattaag cccaaaaaaa
    49441 tgttcaaaag accaaacaat agtagatttc gacactacgt gtcttagttc gatttacatt
    49501 gcacatctac aaagatttcg aagctaagct tctacgcaat tgagcaacac agttgcgata
    49561 tctttgtagt caaaaaattg aaaagcagca tgaaaccacg tagtggtgaa attatattct
    49621 gtattcattc agaccacagc gaaaaacaac tatttcccgg ccaaaataat tattccaatc
    49681 cctatcaaaa tcccaattaa aatcaggcct ttacgtttaa cgttcttctc atgaaaaagt
    49741 aatgcgccta aagagaaaga cacaaccacg ctgccgcgac gcaaggtaga gactatggct
    49801 accatagcat cgggatgaga aagcgaccag aaatacacaa aatccgcaac ggtaaggcaa
    49861 ataccaatga gaggaattgt aaaacgccat tgaaaagttg aaaattgctt gcgtttaggg
    49921 taccatatta tgaaaaacaa caccaacatt agtggtacca tgtaaatatg gtaccaacac
    49981 tgcatggcaa gccggttata gtgatgaaca agaaatttat cgtacaaaga gcttatagaa
    50041 ccaatgatag tagaaacggt cataaacaac acccacttgt tggtgcggaa cgaaatccct
    50101 tctcgcttgc ccgacagggt aaacaggtaa tagaagaaca atgtcaccgc aataccaatc
    50161 cattgccaaa ctgtaagcac ctcgccaaag ataagcagcg cccctattat tgtccacaaa
    50221 ggccctgatg aacgtatggg cgatacaata gtaattggaa ggtgtttcat ggcaaaatac
    50281 gccaacgtcc atgaagaacc aacaatgaca gattttataa aaatcaaaac atgcgcctga
    50341 agcgttaacg aacgaaagta gaaaacagaa tccgagggca catttccaaa ataggcatac
    50401 aaaactattg gcaaacaaat cagcgcactg gtcagcgagg aaaaaaacaa gaccgggata
    50461 actgcattat caactaaaga tttcttttta aaaatatcat aaacacccag caataatgcc
    50521 gacaatatag caagaaggac ccaaaccact tttcaaaatt ttaggtggca aaagtagaaa
    50581 taaaaatagg gaataagaaa aaaggaataa ggcaaaagta aaaaacctcc cagaattgaa
    50641 ttcgtggaag gcttctatac ccataaatgc aaaaagtctt agctgacagc tattttcttg
    50701 ctccaaacat tttgacggac aaagtacact cctgtcttta aagaagggac atcaacagat
    50761 atttcgtttg atgccacact aacgtttgtg taaaaaagtt tcactcctcg cacatcataa
    50821 acagagatga caaaatcact acatacagtt attggaagag atattaatat tcgattattg
    50881 ttataaacag cactaatatc atcttttgta gcatccaaac ggtttacaac tacaggatca
    50941 acatcatctg caggaattat atctacctca ggagtaacaa atgtagagct ttttaaaatc
    51001 tttacctccc cggcatcagg aacaacatca aagataaggt aaacaactgc atcaacctga
    51061 acaatacgaa aaggtacagc aacattattt tgtgtaacac tggcagatgg ccaaccttca
    51121 ggcaatggac ggcggatact cagcggatag ttataaattg aatctgccag gttatcagta
    51181 acctgcaacg tcatggacga atctgtagtc gctgtctcag taacagaaac agcgtttctt
    51241 tctttagaat aaagggttgc atttaagaac gtggtcaccc aaaattttgg cttacgtgca
    51301 gccaagtatg ccacactctt gcgcaattct atagaggtaa ttggtgaata ccctccatct
    51361 ttgtcaattc catgaaacag ataaaccaac catccgttac tcttggcaac agcagaaaag
    51421 tttgtcttga actggcttaa attttgggta gcgcctttat ccccacaaat gacagaacta
    51481 atattgtaat atgaagcagg tgttttccct tcaacaaagc cttggcatcc gcgagctgaa
    51541 atgtaatatt ttccacaaat agaatctact cctgccacac aatatggata ggccatagtg
    51601 aggcactttt tattggtaat ttgaccttca atagcagttt ttgaatcttt tagctctgtt
    51661 tcctgctgag taatggtaac ctggctaaaa tttgcatggg taacagtatg gctggctatc
    51721 tcgtgacctt tgtcagaagc tgttttcaat gctgaccaat ttgcacccga ccaatcttta
    51781 acggtaaaca tcgtcaggtt gaaaccatac tcatcaaata aaggaatagc cactttaaac
    51841 tgattagaac aaccatcatc aaaagtgtag ctgatagcag cctgtctaaa accagcccat
    51901 gtagccactt cataaggctt aggcgctgtt tgtgcaaccc cggtaaaccc gttaacaaca
    51961 agggtaaaag caaaaaataa agtaagtttt ctcatagtat catttttagt ggggcaaaaa
    52021 tagactgccc tccatattca ggcagggaaa aaatgggcaa aaaagtggaa agatttttaa
    52081 ctataatcaa tatttgcatt acaaccaccc cacctcccgc ctgaggcgag acaagccctc
    52141 cttataaagg attagaaaca atgctatctt ctaaagcaaa gcattttaag gtttacttat
    52201 acatttagta aaaaaatcaa aaacagcttt gtattgagat tgccagaaaa cgtaatcatg
    52261 gccagatttt tcgacaagat taaactgatg cttagccttt tcattagatt tcatcaatgc
    52321 cttgaacagc aaagcagact gctcagggtt taccacttta tcatccttgc catgagctaa
    52381 aaataaagga atatttatat tgtttgcttg ataaacaagg ttatatgtag ttttccataa
    52441 atctgcatgc aggttatact ctcccaaata gcctttcagg aggttatcat tgggcatctg
    52501 cagcgggtca taatcccctg aaagggccgc tccggcttta aacaagcggg cattttgaat
    52561 ggcaagcata acaacgcccc tggctcctgt agaaatacca aaaagaaaat tgcactggtt
    52621 ttcttttaac aaaccatact cttgctgtat gtaaggaatt acagtatcgg tcagccaata
    52681 aaccgtatga tactttaccc agtcggcccg agtctgtggg taaaccttta aatgatagat
    52741 agatttctgc atatcaggca agaccaatgc aaaaccttca gctaaagctt ttttacaaaa
    52801 atcacttttc aaacatacat cattataagg aaaattccag cctggcaaca caagaattgt
    52861 tccttttttt aacttttcag gaataacaat ttcaactttt gtattattga taaggattgt
    52921 ggtgtcagtt tgagcgggga tgcctataaa tacagagaaa aataaaacca aaacaatgcc
    52981 atccctagcc cctcctttta aaggaggaga aataacgaaa tgaatatgat tagctattag
    53041 atttattatc ctgcgcatca tcgggtaatt tgaaaatcgg tataaccata aaccatgcca
    53101 tctataacag ccacatgacg gacatgagca tgaggaggac cttcgtaaca ccaattgata
    53161 aaagattcta actgaggcaa tgtaccttcg gcctcaataa aaacacttcc atcaggcata
    53221 tttctgacca agccacatat attgagctgt tcggctacaa tacgtgccgt atgcctaaac
    53281 cctaccccct gcacacgccc cgaaactgta atctttttat gaaaaacagg caactcgtcc
    53341 attacacttt tcttttctgc cattcagacc ttaacagact gtacaattct aaatcataaa
    53401 aacgatcatt gacccactca ccttgtctct cgatgccttc aaacacaaaa cctaaccgct
    53461 gcggtatctt tttgcttttt tcattctctt ttgccacctt aatctgcacc cggttcacat
    53521 taagcaccct gaaagctgca tcaacaaggg tttgacaagc agtagtcaca atgccattac
    53581 cctgatactc ttctgcaagc cagtagccaa tttcagtgcg tttattagcc ttgtcactcc
    53641 ggacaaaacc tatcagcccg actatcacat tttcataaat aataacatac gtcagctcgc
    53701 gggtttcata aggatgcacc aatatacttt taatataact ttctgtatca gaaactttat
    53761 gggtaaaatc cacaaatggc aaccattgcc tgagatattc cctgttcgaa tcaataatac
    53821 caaatattgc aggagcatca gtaacttgaa tttcgcgcaa actgagtttc tcatctattt
    53881 caatttcaag aatacttgtc ataaaattac tttttcataa aaatacagct ttatctgcaa
    53941 acaagcaaag aaatgtgaag aagtaatggt taatggtcaa tgattaaggg ttaatgttgt
    54001 ttagatatgg atgttattta aacgccgaca tgtcagtaag tcgctgtcga gtatgtttta
    54061 actttaacca gacagcgctc gtagaacctg tcagcgtctt atctaagcta cattgaagta
    54121 gattaattat tttgttgaat atattggggc gcaaataata tttagctctt ctaacttcct
    54181 tttttgaaaa aaaaaaaaaa aaatacattt gttttcacaa aaatcttttt atccttaaac
    54241 taaaacgttt gatatgaaaa aactcttatt tattgcactt gctggcatag tattatcctc
    54301 atgtgccttt cattctggca caatcagttc tggccctatt gaaaaaagag ttgtttatac
    54361 cgatatggct gttggtgtag cccaaaccca aaagatattt ggcataggcg gcatatcaaa
    54421 agatgcatta gtgttggaag ctaaaagaaa gcttgtacaa aacaggccat tgcaggaaga
    54481 tgaacaatac gtcaactata ccatagattt taaaacgtct ttctggcctt tttattctca
    54541 aaccactgcc actgttagtg ctgatgttgt aaccccggcc aatactgaca ccaaagagct
    54601 gtattcagac aattacaaaa agaagctctt tgccccaacc tataacaatg acttatttgt
    54661 agtgggcgac accattgtct atgacaactt ttttaaggga caaatacttt cttttgagtc
    54721 gaaaaacaaa gtgcgcatta tgtaccgcac gcaaaacgat aaaatcagga caaaaattgt
    54781 ttctttagat gacatttaca cacaactgaa agagtacaaa acgtacaaaa tgggcggcaa
    54841 tttttatttt gacaaagtaa gcacaggcgg agacaacaaa gtggtaaatg gcaaaatctg
    54901 ggctgtaggg ctgaaatcgg ttttggtata tgattacaat aacaaacttt gtaaagagga
    54961 ttttaaacaa taaaacaaga aaggccgaaa ggcctctttt acactacctc caagctttat
    55021 gcattctaat cgtgacaatt tgtaaagaag atttaaaatg atttaatttg cctaaaacaa
    55081 ggattatgaa aacgataagt gtctctatat cagatttaga atataatcaa ttggggatta
    55141 acaatgataa attatcattc actgaacttg tagatattgt aaacaaagag attaccaaac
    55201 aaacagttaa tagatgtatt caattagcag agaaatataa gttttcacgg atgtctttag
    55261 atgaaataaa tgatgaagta aaagctgtaa gagatgcaaa aagtaatcat tgacacaaat
    55321 gtgctaattt cagcattgac tcaaagaaac taccccaatt ttattcttta caactatgtg
    55381 ttggacaatg ctgtagaagt ttgcatttca gatgagctgt ttaaagaata tttagatgta
    55441 ttaaatagac caaagtttaa taaatatcca gattttatta gtaaagctga aattgtttta
    55501 actcagattg agtcaaagac ttaaaaattt tatccctcca aaagacttga tgtaataaca
    55561 gataatccag acaatagact gcttgaatta gctgtcgaat caaaggttga ttttataatt
    55621 acaggcaata caacagattt tacaatgact gaatttcaag ggactaaaat tgtaactcca
    55681 aaagaatatt gggatttata tagagaataa aacatttgtc ctttgtaaac ctgaaagccc
    55741 ttaacagtca atgcctggaa acgttattaa cagcccaact gcatttcgtt aataaataca
    55801 atatcaggtt aatatcctga atcaaagaga taggctattt ttaccacagt aatagcagct
    55861 attttacgca tgaaaaaagc aaaaaagcaa gaagtcaaag caatagaacc cgttcaggtt
    55921 cccaataaat tcgtccgcaa caaacgcaag actattcttc tgaacactat ggaaaatgaa
    55981 gctttggacg cttatttcaa gaaatataag gtacaaaata aatccaggtt tatgcgcgaa
    56041 acactcatgc gcgttatttt aaaccgcttt gccaacgatt atcctacgct ttgggatcag
    56101 ccaggagtgc ctgcacactt aagacctttt taaccaatta gcgaattagc aaatgtgata
    56161 attagcaaat gaaaatacaa aatattgtca attagttaat tcgaaaatgg gaattcaatt
    56221 agctaattct cgaattaact aatttgctaa ttacaatatc attcccgccc ctaccgtttc
    56281 gtttgttcct tcttcaataa gtacaaggct tccggtaatc ctgtttttct tataggcatc
    56341 ataagccaac gggcgtgtgg tttttattct cacacgggct atttcgttca tctttagctg
    56401 cttttcacca gtaagctcgt ccagggtatt gatatcaacc ttaaaaacca cgtcactaat
    56461 catgcaccgg gcatcggcag tggtatgcct gaggtgatat ttggctccca cattcatagg
    56521 ccgttcgtta agccagcaaa gcataatatc aagctcttta cttaccaatg gcagactgtc
    56581 aggcttaacc aacatgtcgc cacgcgaaat gtcaatatca tcttccaatt gaacagaaac
    56641 agactgtgga gcataagctt cttcaacaga tccattaaac acatcaatag atttaatcac
    56701 agactccagt ccagaaggca aaacgaccac cgcatcgcct tttttaaaga cacccccggc
    56761 tatacggcca gcatacccgc gataatcgtg atatgtatcg ctatatggcc tgataatata
    56821 ttgcaccggg aaccttgcat cggtatggtt gatatccccg gcaatatgaa cgttttccaa
    56881 tatatgcatc aatgttgggc cgttgtacca atgcattttc tctgacctct caacaacatt
    56941 atcccctttt aacgcactaa taggcacata ccaaacatca cgcacgtcta actttgatga
    57001 aatggcttca atctgcgaac atatctcatc aaacctcgac tgagcgtaat ccaccaaatc
    57061 cattttgttt atacaaaaga ctatgtgggg aatacctaac aatgaagcaa tgtaagcatg
    57121 acgtattgtc tgctccacaa caccattacg tgcatcaaca agtattaatg ccagattagc
    57181 tgttgatgcc cccgtaacca tattgcgggt gtattgtata tgcccgggag tatcagctat
    57241 gataaattta cgcttgggtg tagcaaagta tcgatacgca acgtcaatag taataccctg
    57301 ttcgcgttca gcccttaaac catcagtaag caaagcaagg tttacatatt cttctccacg
    57361 tcttttgcta gcctgttcaa gtgcttcgag ctggtcagca aagatagcct tgctatcata
    57421 aagcaaacgg cctatcaacg tacttttccc atcatcaaca cttccggcgg tagtgaagcg
    57481 caaaagctcc atatctaaat atccagacat actatttttg aatcaaaaac caagagtcaa
    57541 gaatcaagaa gatttcaaaa ttcatagaaa ctaaaccatt ggtcattagt aatttgtaat
    57601 taatcattaa aaatatcctt cttttttacg gtcttccatg gccgactctg agcgtttgtc
    57661 gtcaaagcga gttccgcgct cagtagtacg cgaagcagcc acttcattga taatatcttc
    57721 aaccgtagag gccggagact caacacctcc tgtacaagtc atatctccta tggtacgaaa
    57781 acgcacctgc atgcgagcta atttatccga aggtttcagc ataatatatg gacactgagc
    57841 aagcagcacc ccgtcgcgaa tgatcatatc ccgctcgtga gtgaagtata agcttggaag
    57901 tggaatattt tccaaataaa tatattgcca gacgtccaat tcagtccagt tactcaatgg
    57961 aaacacccga aaatgttcgc ccatgtgttt acggccattg aaaatgttcc acagttcagg
    58021 tcgttggttt ttgggatccc attggccaaa ttcatcacga tgtgaaaaga accgctcttt
    58081 agcgcgagcc ttttcctcgt cacggcgacc gcctcccata gccgcatcaa acttaaattc
    58141 ctcgatagca tccagcaatg tcactgtttg caatacatta cgactggcat taaagccttt
    58201 ttcttccact accttaccgg catcaataga gtcctgtaca agacggacaa tacattgcgt
    58261 cccggtatct tccatcagct tatcccgata gtcaagggtt tcctgaaagt tatgccctgt
    58321 gtcgatgtgc aataaaggaa aaggaacctt agctggccaa aaagccttac gggcaatatg
    58381 aaacatcacg atagaatctt tgcccccgct aaaaagcaaa gcaggccgtt caaactgggc
    58441 agccacctca cggataacgt acatcgactc agactctaac tcccgtaaat gattgattgt
    58501 ataattcttc attatagcgt aattcaacag ttacaaacaa caatttaagc actataaacc
    58561 agttgcacaa attattgttc gcctgcgaaa ttaataaaaa gtagccactc gtaaaataag
    58621 agttaaaaat atttatatct gattatattt tttaaaagaa ccaaaaattt ctagttcttt
    58681 tcacaacctc acttttaaaa aaaactattt cacattagta acttctatgg ttttcttata
    58741 ttttttcaat attttatcta ctaacttatg cctatttact tccattgaaa atgacttggt
    58801 ctggacttta ttactgtaat acaaaaattt gtgttccttt ttatccacca ctacaaatgt
    58861 tgcgtcaata actgatggaa gcgggatcat atacactgta accatcccca aagttaaaac
    58921 acttgttaca accattaatg taattgaagc agccatgctt cttttaattt gattattcat
    58981 aaatccatta taatataaaa atccataata acggccagga tatttactaa taattgaatc
    59041 caaatccttt ccaattggaa ccataggaaa tacgttattg tcaatttttt ttattttctc
    59101 taaagtccca ataatattat cttgagtctt aaagtcattg atcagcattg aatcatttat
    59161 aatgattcct ttagctggga attgcccaag aattaattta ttcaatgtat ctctcacaga
    59221 ttgggtgaat tcgggagagg tttgtctttc attattctta attgttgtaa caaaaccaaa
    59281 tggagaaaac aaataaatag tatcaatttg caccccaaca taatttggat tcactttttg
    59341 agcatttaca cataatgaga ataggagaat tgcaattaga aaattgattt ttttcatatt
    59401 ttactaggtt tttgagcaga aaatgattta ttggggaata aaggtaagta atacctcaga
    59461 tttatattaa acgttttaag gtcaggaaaa ccattatcag caactctaat attatatcca
    59521 gcactgattg ttaaaaaatt aaaaccatat ccaaattcag gacgaattac aaataagttt
    59581 tttttatagt ttgtatacaa aataggacat attccaaagc gcaaaggttt taaaatagaa
    59641 ataagatata ttttatagcc atcaatattg ctagagaaat ttcttaacca agatccaccg
    59701 taaaagattg tactatttac agtataagga ttaaactgat gcttgtcatt aagagtaaaa
    59761 ctgcccctcc caaaacctgt ttcaacttca agaccatggt tgtaagtcaa acctccaata
    59821 aaaaacagtc cttcataata gcgcataaaa tcatagctct tagctgcagt ttgtacttct
    59881 tctaatttat ttttttgaat aaaacaacgc ttataaaaca agctctgcaa actcattatt
    59941 tgtgcaacaa aaacaggtct attttcatac tttaaagcta tactcaaaga tgtgtcaact
    60001 atttgctttt tcaaaatatc caatgtatct aaatgcttta aatatgcacc tttattgtcc
    60061 ttcaaataaa actgggcttt ataataatac aaagaagctt tttgaggttc atttgctatt
    60121 gccatgttta tccaatagaa tgcagaatct aaatggccat taaattgagc tttaaaacca
    60181 ttttgcgcat aactactact tattgacaaa ttaggtgaaa gctttttcca agatgggcaa
    60241 ggacaataat catagggaat acaattcctg tttttatcaa tataatacca acttgaatat
    60301 tcaggttcat tattaaaatt agaactttca attatctgtc ctaaatgtaa agggttataa
    60361 ttctctataa acaattgttc attggcaaat ggaattaaaa tattatctaa tgtatcaata
    60421 gctccatacc taaaatccct tgattggaca attattacag aatcattgta attctgaatt
    60481 ctattatatt tagctggaat tagaatctta ccagtatatt tatttttaat tccatactta
    60541 gcttcagctt caaaaggaac aatattttga gaaaaaacag atgttttggc caaagtatct
    60601 attaagacaa ttactaaaaa tatcttcatc atatttattc aagttaatat aacaattacc
    60661 ccctaattac tatatgtatg cacgctattt gccgcagatg gcaggtagtt aataccaaac
    60721 caggccacaa taagcactac aaacgagatg aaaagcagga aatacgccag gttctcgttc
    60781 tgtttatgac ggaaacggat gtgaatataa acaagataag ccaaccaagt caggagtgcc
    60841 catgtttctt ttggatccca ggtccagtaa tgtccccagg cttccttagc ccagaaagca
    60901 ccaaaaatca gacccaatga taaaaatgta aaacctacag acaccaaatt atctgcaagt
    60961 ttcaacacat catcattgac atgtctgttt ctgaaaaaca tttcaaacaa tgctttggct
    61021 gaaaccaaag cagaagcagc caaaactgca taggaaatca tatacacaat tacatgcggc
    61081 gcaaaccatg gactttgaag ggctggcatc agcgttttgt cgtgtgcttc aacaaataca
    61141 atgttcagga tgaggaacat caaagccaac ccacagctat aagggacaat ccatttgata
    61201 ccccaacgca aataagtaag ataaccggcc aacaccaaaa agaaagcata ccaaagacgt
    61261 gtttcgccta atgtccgtaa aggcaagcgg tcaagtccgg cccaaaacgc tcctataaaa
    61321 gttccaagaa ccgctaaacc agttattata gcagcatttc cccagagttt attttttgaa
    61381 gtatataaaa acacgcttcc tgccacataa aaaagagcag aaacagcagc aaaatatgga
    61441 aattcaagcc aagtcataaa ttaattgtta attgttaatt ataaattgtt aattgatagt
    61501 aatcaaccat ttatcattta tcatttatca tttatcattt atcatttatc atttatcatt
    61561 taccatttgt attcagtctt ttttcttttt tctttgtcca aaagctaata ataatgccag
    61621 ccatacttag tataataccc agataaacgg caggaaccca cgggtcacgg acagcttcaa
    61681 taatactaag ttttgaaaac ttcccttttt ctttatcata gccggtttga taaagcttcc
    61741 atccggctat agcaaatggt ttgttcactt caagcagaac attcttctta atgtcattat
    61801 gaacaaggat ttcaattttt gaactaaact ttttcggctc gggcaaggtc attgctaaaa
    61861 gataagtaga atcgaggtaa aaaacaccgg cagactggac aggactactg ctgtttatcc
    61921 aggcttcctt agtctggccg gttttgttat tgaaacattt tacaaaagca gcctgcacag
    61981 ccaaagaatc ttcagacaca acaaaacctt ttgaagagtc tgcctttgct gatggaagat
    62041 attctacaat ctcaatactg aaatttttga gtttaaataa ttttcctttt tcaattaatg
    62101 gaagattgtt tttcaccttg ctttcaatct ccattgtcgc acttttaacc aatgcaagct
    62161 tggccggaaa ttcgtctatg ctgaaatcat ccagtttaat agcaaacggc aaggtatgcc
    62221 aactgtaatc cttatcataa gccatattaa cagtctgccc ttcttctacg gcaacctgga
    62281 agcgttttag gtcagacgaa gacaacccgg ctgccataac aacaatccac atccccagat
    62341 ggctggcaac aaagccaata tccgcgactt taaaagttga aatccgccta acaacaacaa
    62401 gaccaagtgt tgtaagcaga tatatgctgg aaatagtaaa cagccagctc ccttgcaatt
    62461 tattcagttt aagaaccatt gttggctggc cttctaacac ttgaggcact acagccatag
    62521 ccaatgcaat aagagaaaat aaaacaatgg cccccaaaca agcaggaact tcggacaacc
    62581 agcgaacaac ataaattttg cgaaatgaaa aataaacgca cgggataagt gccataaaaa
    62641 caagcccaag aatggcgtta tatggataaa ctggcatttc cacaatggcc gcagaaagca
    62701 tatccagcat aactccaatt atcataagtc ctgcaacaat agcagcactt tctttatagc
    62761 cccagggctt ctcccatgtt ttgcgtttaa cttctgccat taataattag atttcaataa
    62821 taaataaacg tcttaaacac caaatataaa tattttaaag acaaatatcc ggcagaattg
    62881 acaaagcatt aactcataaa taaaaaagct tcgacattcc acaataaatg gcaaatcgaa
    62941 gcctaagccg aaaaaaaacg tttcttactc aaaagcattt acagcgcaat gtagaaccct
    63001 gtttcctgca agatgataag caattctgag cgactcagct atttctgctt ctgttgcgcc
    63061 catctttcgg gcaacatctg atatcccctt taccccggac gaaccagcat aagcgtccaa
    63121 agctaaaaga atcagaaatt ttgttttaac atcaatagca ccgggagcca acactttgtc
    63181 atgctgggca gataccatct gaaaaaattc cgaatcaact tttccaagtt tttcaatgaa
    63241 tggaggatag tgcttcataa tcatttattt aaagtttgac aatgtttatt tccacaaaag
    63301 aaccaatagt cccgttcagt ttcgttaccc tatggtaata aatgcaacaa ggtcagactt
    63361 tgcacatttc gcgcctgata cggctcaacg actgaggtgt aacgcctata aatgatgcaa
    63421 tgtaatattg cgggatgcgt tgttggatgt gcctgaagtc cgaaagaaaa ttcaaatacc
    63481 gctgctctgc tgtagctgta aacgttgtga caatatgatt tattgctttg gcaaaagtca
    63541 tttcgaggta taaacgaccg aaacgctctc cggtagagac ttttttgtaa aacaattgca
    63601 tattgttgta tgaaatggta taaaccacgg tatcttccaa tgcataaaag gttttctttg
    63661 aaggtgtgcg atggataaag ctttcaaagt cgcaaacaaa ttcattttca gcactgaaaa
    63721 agaacgtcac ttcctttcca tcgttgttaa cgcaatgccg cagcaaacct ttttcgacaa
    63781 aaacaaactc tttacaaact tcaccttctg tcaacaaacg ctcgttttct ttcagctctt
    63841 tctttacaaa aagctgttcg ataacatcaa tatcctcatc ggtaaggcta acaaaacgtt
    63901 ttatagcatc aattagggtt tgcataaata ataatcagac tttcaaatat ataacaatat
    63961 taaaatctat cacctttttt ctccacctct attaatactc accagataca ccccaaagca
    64021 aaccagcaag cctgctgaaa tgtttatcca gtttaatgtg tctgtaccag tcagaaaagc
    64081 cataacaaca gacaccacag gctgaatgta aatataaatg ctgactgtag taggactaag
    64141 atgcctaata ccataattga taaggaaata agtcagcact gttgtacaaa taacaatata
    64201 gaaaagggct gcataaatac tcattggcag ttgtgaccat tgcacagcca acataggctt
    64261 aatgccaaac ggcaaataaa gcagagagcc aaataagaag gtccatttca gcacaaaaac
    64321 cgagtcgtac ttttgcatca ggggtttggc gtacatcatg taaatggcaa atgatgcaga
    64381 gttgactaaa aggaggatgt cgccggtcag atgctccggg ttgtaagcca caaggccttt
    64441 actccctgcc aacaacaaag cgcctgaggc acctattata atccctgaaa ctttcagttt
    64501 tgtaactttt tccttgagta tcagagccgc cataaccagc acaaacacgg ggttgctggt
    64561 aataatgata gccgaatcga taggcgatga caagctcaag cctttgataa aaaggaattg
    64621 gtttaagaat acgcctaaaa gtccagcaat aaaaaggttt ttatattcag gccttccatt
    64681 taccatgcta ttgcgagtta tgaaagcaat ggcccaaaac aacattgttg aaggcaggat
    64741 gcgcagaaaa ataactgctt caggacttat atagcccatt gtctttttgg cgataacata
    64801 gttaacgcca tacaaaaagt tacctgcaaa taaagccaaa tgcgctttga acctaaggga
    64861 catagttttg ttttagattg caaaagtggc attttttttc aagctttaac aaggtaaaaa
    64921 aacaaacatt taacattaaa cactcaaagt cttaaagaaa atgagctaac ttgccacctg
    64981 tttattaagt attacattca ttctaaaaaa cattcaccca tttttaatct aatactttaa
    65041 gtaaaatcat acaaaaattc ttttggtctt aaaaatttag ttcggatgaa tgatgtagat
    65101 taaaacaaaa acctaaattt gttggcgaaa aatgcccctt cagcgtgata gagaaatcag
    65161 acatagaatt attcaaagaa attaaggaag gaaagatgtc ttcgtacgac aagcttttca
    65221 tgacttatta tcaaccgttg tgccgttttt ctttgtcttt tgtaaaagat gaaaacgctg
    65281 cagaggattt ggtgcaggat ttgtttgttc atctatggaa caaccgagcc gtaatggata
    65341 tcacgaattc ggtaaaagct tttttataca ccagtgtaag aaacaggagc ttaaactata
    65401 taaaaagcca ggcagcccgc aaaagatatg aaacggtgca ttacgaaaat ataagttctg
    65461 atgctgttga ggaagtagaa gataacatgc ctgaaaagct tgtgctcata gaaaaagcaa
    65521 tcaatgaatt gcccgaaaaa tgcagggaga tatttgtgct aagcaggcat aaaaatatga
    65581 aataccacga gattgccgaa catcttgatt tgtcgcccaa aactgttgaa aaccagatgg
    65641 gcattgcgct aaagaaaata agggagttca tggcaaagca catgagtatt ttgatattga
    65701 tattttatgg aatttttaaa taatttttgg gggaaaatgt ttttgtttgt gtctcaatat
    65761 aaaattacgc ttcatgcatg aaaatgtgcc atacgacctg attgcttctg ctttggaagg
    65821 agcctgtaat gctcaggagc agaacgagct ggacttgtgg atagcacagt cgcctgctaa
    65881 cagggctatt tatgaagaga tgaaaagttt atgggccgaa agccagcaaa tagctgacaa
    65941 tttcagtcct gatgcccatc aggcattggc cagagtacat caacgcatag gagcaggaca
    66001 taaagcaaaa gtattttcat tgaacagtat ttcatgggct tacaaaatag ccgcatccat
    66061 attaatcgtg gcaggagcgt tattcttttt tgtttccaat aaaaagccag tacaattagc
    66121 agcaattact gataataaga gcaatagcga aattgtgctt tcagacaact ctgtggtcaa
    66181 actgaacaaa aactcaaaac ttacttatcc agagtatttt aagggtaaag aaagaaggct
    66241 caggcttgaa ggtgaagcac tattcgatgt tacaaaagac aaagaaaagc cctttgttgt
    66301 agaagtaggt cagacaaaag taaccgtgtt gggtacgtat tttaatgtca gggcttactc
    66361 tttagaagac agcatagtcg ttagcttgct ggaagggaaa gtagaatttg ccattgaggg
    66421 aaaagatgct aaagttattt taactgccgg cgaaaaaggc ttgtatatta agtcaaaagg
    66481 ttcattaacc aagatggaag agcaaaatac caatgtcacc agttggaata cagggatatt
    66541 gaagtttgaa gcacttccat tagccaatgt ttgtacaaca ctttcggaac actatggaat
    66601 ttctatatgt atcaaaaacg catcagatgc caatctaagg ttcacaggta catttaaaaa
    66661 tcaacgcttg tcagagattt tactttctat tgaagaagcc acaaatttga aagctgtcaa
    66721 aaacggaaat agtattattt ttgaataaaa aataattgcc cctttttgat gcagcgtatc
    66781 cttttatttt tactgtttac atttattata tctgttgaga ttattgccca gcgtgatatc
    66841 ttaagtcgga aagttactgt ttatgctcaa aataaacgta tagccgatat tattaacgat
    66901 ataagcaaac aatcagaagt agattttttg tatagccctg atttggtaga ccttgaaaga
    66961 caaattactg taaacatcac caatgttgat ttacaaaaag tattacaaca gctttttcgt
    67021 ggcacaaatg taatatatag tattgtagcc ggccaggtag tactgaaaaa gaaaattcaa
    67081 ccccctaaac attctatatc aggttttgtt agcgatatgg ccagtggtga aaagttagct
    67141 tatgccacta ttgcgataca aggcaaaaac tttgccgtaa ccagcaataa ttatggtttt
    67201 tatagcctat cagaaccaga aggctttgtt tatatgattt tcgtatgtag ggtataaatc
    67261 caatgttata ggtattgaac tggataaaga caccgttatc aatataaggc taaacctgga
    67321 tacaacgctg gtaaaggata ttattgtcaa tgacaattcg tggaaacaaa gcttaaaaaa
    67381 ttcgcaaacc gggttaaaca ggttgagtat gcagtcgctg cgtcagttac cccagttgct
    67441 tggcgaaaat gatttgatga aaagcttgca aaccttacca ggagtacaga ctaagagcga
    67501 aggcagcagc gggatgttgg tacgtggcgg gaaccatgac caaaaccttt atcttttgga
    67561 tggcgtacct atttacaata ttaaccacct gtttggcttt gtttcggtct ttaacacaga
    67621 tgcagtacag catgtgtcga tgtataaaag tggtttcccg gccagataca atggcaggtt
    67681 gtcatcggtt gtggacatga aactcaaaga agggaattcg aaaacatacc aggggaagct
    67741 atccattgga acattaactt cttcagcttc tgttgaaggg cctatagcaa acaggggttc
    67801 cttttttatt gctggcagac gtacatacat ggacctactt ttaaaagcaa tggcaattac
    67861 cgcaaaattg aatgaccaac aagataccat gttgttgaat tttgatgcca atttttatga
    67921 tttggtagca aaagcaaatt ttaagttgtc cgaaaaaagc cacatttatg ccagcaccta
    67981 ttttgggcag gatatatacg gggccgaagg aaggcttaat ggtgatgatg agagcggctg
    68041 gctcaaatgg gacaaccttg tcacagcgtt caggtggaat tacaaaatca ataacaagct
    68101 ttttgtaaac aataccctta cctattccaa ttttagcttt gactttaatt taaacctgaa
    68161 tggttctgac agcctaaaac gaaaaacaag cctaaaatct gatttaaaaa ccgggattaa
    68221 agattgctca gtcatgtcag actgggaatg gtcattgaat gataaacaca atttacagtt
    68281 tgggcttggg gcaacttacc atgatttcaa tgccggagat ttgagatgga ccagcaattt
    68341 cttttcaaca atagacagca tcaacgtcaa aaatgcagct ccttatgcta aagaagcata
    68401 catatatgca gaagacacat ggaagttttc ggaaaaagcc ggttttacta tcgggctgaa
    68461 ttctgccttt tttaatgtta ataaaaatat agactttgtt cttcagccca ggataagtac
    68521 attttatttg ttgaccgaaa atttatcact aaagtcgtct tactcgcgta tgggacagtt
    68581 tttgcacatg ggttc
//
LOCUS       DAOSPF010000010        67737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_436_length_67737_cov_6.623312, whole genome shotgun sequence.
ACCESSION   DAOSPF010000010 DAOSPF010000000
VERSION     DAOSPF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 67737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_436_length_67737_cov_6.623312"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1409
                     /locus_tag="PK252_03440"
     CDS             225..1409
                     /locus_tag="PK252_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT13796.1"
                     /translation="MMRINASSSKELKANFNSYTAQLLAFLLTLKSSQHELKKFMIWL
                     NHLITCEAILMTMKYLLMLTLTAILIINGCIQKQNDKIKIGVMFSNLKELRFQKESSI
                     MEQFIKEKNYEYLLRDAENDPSKQERQFAELKNEGIKVLIISVVNNNVGKSIIKEANK
                     NGIITIMYDGFIGSEFLNYCIRFSDKEVGKYMAGYALKNVPEGNYMILGGDGRNENAP
                     LIRSGIMEVLNTSIAASKVKIVYNNFMEEWSADEVYAAVKKYYKLSAEERVDAIILSS
                     DNMATGAIKALEELKPTKWPLITGQDGSLTACLNILQGKQSMSVLKDSKKIAGQTALM
                     AIQCAEGKTPETNGFTTAKGNTGEIKIPSFFIDLEVVDSASIYGLIKRGIYTKESLGL
                     VN"
     gene            1563..2480
                     /locus_tag="PK252_03445"
     CDS             1563..2480
                     /locus_tag="PK252_03445"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOT13797.1"
                     /translation="MIKSRYLITTYILSLILSSVYAQKNEIKDSIFSVNNNTYKALGL
                     TTAYYGASMYVLSKTWYRDKDLVSFHFYNDNSGYLQVDKFGHTWGSYVYSYIGYHYLI
                     NSGFSRKEALFYGATLGFTLQAPIEIMDGIHQGYGFSLGDIAANALGSGLVIGQELLF
                     NEQIIKYKFSYWESSYAHDANGYLGETSLNRMLKDYNGHTYWFSFPVNKFFKSQFIPE
                     WLNIAIGYGANGMFGEFENISSYKGVDIPKTIRYRQFLISLDIDWTKIKTSSKMLKII
                     FKGLTFVKLPFTTFEYNSMGKSKLYLIYY"
     gene            2736..3179
                     /locus_tag="PK252_03450"
     CDS             2736..3179
                     /locus_tag="PK252_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13798.1"
                     /translation="MKIEKINENKKQFLDLLLLADESEIMIDKYLPSGDLFALYDDDL
                     KSVCVVAPINSETCELKNIATYEKYQGKGYGRALIQFISDFYKNDYKTMLVGTGETPA
                     ILSFYESCGFEQSHRVKNFFTDNYDHPMFDGDIQLVDMIYLKKSL"
     gene            3304..3624
                     /locus_tag="PK252_03455"
     CDS             3304..3624
                     /locus_tag="PK252_03455"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT13799.1"
                     /translation="MIDSNKINDIVYRIATKFNPDKIILFGSYASGSQNEDSDLDLLI
                     IKDTDLPIQSRGFDIRMSLIGTKIPFDILVYTNLEFEKEKSNSFSFLNSALKNSKTLY
                     ERAD"
     gene            complement(3749..4000)
                     /locus_tag="PK252_03460"
     CDS             complement(3749..4000)
                     /locus_tag="PK252_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HOT13800.1"
                     /translation="MLVISSRELRQNQKMYFEKADKGEQIIVQRGKDKAYALTPVTED
                     DIYFNAEMVERIKQSIKQVEEGKVKRVTTPEGISNLLGL"
     gene            4506..4859
                     /locus_tag="PK252_03465"
     CDS             4506..4859
                     /locus_tag="PK252_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT13801.1"
                     /translation="MISFSENLKRVRTQKGISQEELSKKIDIHPVQFSRYERAQSVPS
                     IDVVQKIANALEVTIDELVYGNQNNKAEQNIKDRELLSMFSKIQLLNEKQKETVKDFL
                     SAYILKVDLKEKLIV"
     gene            4864..5082
                     /locus_tag="PK252_03470"
     CDS             4864..5082
                     /locus_tag="PK252_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13802.1"
                     /translation="MQTLLVEVKDNTGLKILQELEQAHIIRLIQTAQEKIPQRLSKRL
                     RGAFSKETAQQMKSELEQMRNEWQQRDI"
     gene            5061..5453
                     /locus_tag="PK252_03475"
     CDS             5061..5453
                     /locus_tag="PK252_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT13803.1"
                     /translation="MATTGYLIDTNAAIDYLGEAMPQSGLAFMDNIIDKEYNISVISR
                     IELYAYSKLTEKDKETLDIFTGQASSLNINDEIIEKTIELRKTHKTKLPDAIIAATAL
                     VCNLALVSHNVKDFKNITGLSVIDPYSL"
     gene            complement(5621..5848)
                     /locus_tag="PK252_03480"
     CDS             complement(5621..5848)
                     /locus_tag="PK252_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13804.1"
                     /translation="MGNLFVKHGQRPNIMRRSGTLRRTGAVEQVIQEIINNHFDKLDK
                     GKPVGIKIEVKSNKVTSTSKTYNDYKLEIEK"
     gene            complement(6704..6777)
                     /locus_tag="PK252_03485"
     tRNA            complement(6704..6777)
                     /locus_tag="PK252_03485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6741..6743),aa:Pro,seq:ggg)
     gene            complement(7009..7776)
                     /locus_tag="PK252_03490"
     CDS             complement(7009..7776)
                     /locus_tag="PK252_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOT13805.1"
                     /translation="MMTIKYDGTFEGLLTLIFECYEQKLEPQSIIRDNSSNNNLFSYE
                     YFVNTDEAKAKRVWNGLHRRISAVTCQMLYYTFLSEERNVEMLILHYVRKVFASNITI
                     EANFGDADVLEMKKIAKKVNKEAGRVPMFVRFQKTADDIYYASYDPQYNVLPLVTKHF
                     EERFADQRWIIYDTRRNFGYYYDLKTTSEIRFGESKVNTADGSFAEEFMAAEEKIFQE
                     LWKNYFSAICIRERLNPKLHVQLLPKRFWKYLIEKQA"
     gene            complement(8323..9606)
                     /locus_tag="PK252_03495"
     CDS             complement(8323..9606)
                     /locus_tag="PK252_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOT13806.1"
                     /translation="MVSEKTLEKVKILAGAAKYDVSCASSGSSRKNSGKGLGNAAPWG
                     ICHSFAEDGRCVSLFKIMLTNNCIYDCAYCLNRRTNDVKRTALTVNEVVELTMSFYRR
                     NYIEGLFLSSGVIVNPDHTMAMMVRVAKDLRTVHNFNGYIHLKSIPGASDELIREAGL
                     YADRLSVNIEIPTEQNLKLLAPEKDYKGIFMPMNAIRQNILVSKEEKMKYKSAPRFAP
                     GGQSTQLIVGATPDSDKQILDLASSLYQQQQLKRVYYSGYIPINNHDSRLPAIVQPPL
                     VREHRLYQSDWLLRFYNFRVDEIVNEKYPDLDLDIDPKLAYALRNPWIFPIDINKAPY
                     EMILRIPGVGVNSAKMIVMSRRYGKINSLQLQKMGVVMKRAKYFITCHELASPTVNEL
                     TPDKLKVLLTEKSTKKANENLPKQLELFTDAHFAN"
     gene            9871..10851
                     /gene="trxB"
                     /locus_tag="PK252_03500"
     CDS             9871..10851
                     /gene="trxB"
                     /locus_tag="PK252_03500"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT13807.1"
                     /translation="MENLDTPQSSNSALTGEAEKVKCLIIGSGPAGYTAAIYAARANL
                     SPVVYEGIQPGGQLTTTTEIENFPGYPSGVTGTEMMEDLKKQAQRFGTEVRFGIATSV
                     DFSKRPFKVVIDENKLIEAQVVIIATGATAKYLGLESEEKFKGSGVSACATCDGFFYR
                     KQDVAVVGGGDTACEEATYLANLCNKVYMIVRKPFLRASKAMQERVFNTPNITVLFEH
                     QTKELIGKDAVEGALLTNAKGELVKIDITGFFLAIGHHPNSDIFKPYVNTDEAGYIIT
                     KPGTSQTNIPGVFACGDVQDKNYRQAITAAGSGCMAALDAERFLGEHGLA"
     gene            10942..11715
                     /locus_tag="PK252_03505"
     CDS             10942..11715
                     /locus_tag="PK252_03505"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent NADP oxidoreductase"
                     /protein_id="HOT13808.1"
                     /translation="MSKLAIIGAGNVANHLSKAFQKAEHDIVQVYSRTQGKAQALSSI
                     LNCSCTIDISALTDDADIYLFCISDSAIEEVLEKKDWHNKLLLHTAGSLPLEIFKNRS
                     SDYGVLYPLQTFTTNFTLDITKVPFFIEGNNYTTLKTIKELASSISPQIFEADSQKRL
                     QLHLAAVFANNFTNHMMAIASQLLEENNIPAENINPLLEETFKKAQTIGADKAQTGPA
                     IRNNKAVMELHNHLLAKHPEWQKIYTFVSESIKKNTIHG"
     gene            11708..12229
                     /locus_tag="PK252_03510"
     CDS             11708..12229
                     /locus_tag="PK252_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT13809.1"
                     /translation="MANYREKLTKIKAFVFDVDGVLGSDKVLLFPDGDMMRSMNIKDG
                     YAMNYAIRQGYKIAIITGGTSKSVKHRFTRLGIDDVYLESSNKIKDYEHFLEKHGLKN
                     EEILYMGDDMPDYKVMKMVGVATCPVTAANEIKEISHYISDKAGGEGAVRDVIEQVLK
                     VQNKWISAESFIW"
     gene            12253..13200
                     /locus_tag="PK252_03515"
     CDS             12253..13200
                     /locus_tag="PK252_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOT13810.1"
                     /translation="MKHFIKLIRYKNLAIIAGVMYILRYGLILPLLTSNGLANQLTHI
                     QFLLLTIATVFIAAGGYIINDVYDVDIDKINKPEKLIIGKYIRLRTAENIYVGLTLIA
                     IICGAYVSYTVNLRSLSLLFPIVAGLLYFYSTSYKSTVLLGNVAIAAITALVPFMVII
                     FELPLLTIKYASFTGSSFNFNFIIAWIAFYSAFAFISTLLREILKDMEDFEGDEAYGK
                     KTIPITWGIKISKYITLSLSLLLLCLEIFILIKYLHDLFSVIYIIPFVITPTIFVMVK
                     ILSSKTKEQYHLASKWCKITMATGILYIIFVRVLILGLY"
     gene            13326..14438
                     /gene="lpxB"
                     /locus_tag="PK252_03520"
     CDS             13326..14438
                     /gene="lpxB"
                     /locus_tag="PK252_03520"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOT13811.1"
                     /translation="MKYYIIAGEASGDLHGSNLLKALKEADHSAEFRIVGGHLMEEQG
                     GTLLKHYKDMAFMGLDVVLHLRTILRLMKYCQQDVLAWKPDVLILIDYPGFNLRIAKF
                     AHSNGIRTYYYIAPKVWAWKENRVKQIRKFVDKLFIIFPFEKEYFAQHNIQSEYFGNP
                     LNDNIHAFKNSEDGFNIIASQYGLEKGKYLALVAGSRRGEVKHLLPPMIKALEGLNQY
                     KLVITAAPSIPLEFYQKIVGNKPIAIIYNQTYTLISNAAAAAVTSGTATLETALLKTP
                     QVVVFKTNWPTYCVGKMLVKIKFFSLVNIIQGKEVVKELLQVNLERNIRNEISLILND
                     NNYKQNMLNNYEEIAQKLGEPGVAKRVANRIIELLK"
     gene            14441..15625
                     /locus_tag="PK252_03525"
     CDS             14441..15625
                     /locus_tag="PK252_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT13812.1"
                     /translation="MIKKVLFVLIIMLPFVKSPAQTLRIGLFSNITTKATVFSAVQGS
                     YLLSGDNRAVLTLKEKQNILLVKKGDSVTCICADKQKYTFKKVKLSHISLNAFFSLRP
                     IDPSNELHYYDDDLEIYADLGKLQTINIVDIDNYLAGVVEAEAGNNALPEFYKAQVTL
                     CRTYTLSHLDRHNEEGFYLCDGVHCQAYHGKCSGKNAIVDAARTTHNKVIQDNDSVLI
                     TAAFHANCGGETESAQNVWLIKRNYLAPIKDPYCQDRNNSRWERKIPIDQWIKYLISN
                     GFTINDQISLSLLNNTQFSRKQYYKIGTDSLTYRKIRNDFQLKSAFFSVEVKNPNIEI
                     HGRGYGHGVGMCQEGAMQMAVLGYTYEQIINFYYKNVKIIDFSNINANKNPTIKVLYT
                     NP"
     gene            complement(15632..18097)
                     /locus_tag="PK252_03530"
     CDS             complement(15632..18097)
                     /locus_tag="PK252_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT13813.1"
                     /translation="MNNPVNPKFIDVLQIGLFDLSADGKLLYIDAKFSGLWGLDTNEK
                     VPASVYEMLSEGEADYLIGEMKRKEVIRVRSTFSLNGKNIEAFVVGMPLDENRYNFLF
                     IPANQWYDQRVLKDKNGFFNSSGMNDNLMFELTGMKEKLKQLMSYNEILASKIVEQEL
                     VDKKIFTHINEGIWEYEFKTKKFSFNDRFLEILGYEAFEFEQDIATLHKLLHEEDKER
                     IIKAIEYTGPDASNFILECRLRNQKGIYQWVQINAMLDEGFEDANSRKWLGTVADIDI
                     RKSYEEQIRQNEQILAEQSESFEKVNSQLEISLKKINKLYTQLQEKQKLLDNILNSVP
                     AFIGLVSDNIILFANEHASLMTGYNMEEISGRNFSFLFADEDEYLNVNKILSTYLVEQ
                     NTSTVNTHWRMKNGVRLDVMITAANLELSGSNSFSFVAFDITSQRIYEEELIAAKEKS
                     EVAERLKSVFLCNISHELRTPANGIIGFAELLKNCRDDEKRAQYIQIIINSSKQLVKI
                     VSDLVDVSKIEIGEIELFSNIVELPKLMGELFEYFKNYMLIRGKTQIELVLEPLDALL
                     KEPVVTDEPKLRLIMQNLLSNAVKFTDNGQIRFGCSKREDYLEFFVSDTGIGIAEEEK
                     ELIFEAFKQVEMSSKRLYGGNGLGLAIAKGYVKAMGGTIWVESKKGGGSKFSFIIPYN
                     VKSQVIIKADNGASFKWEDKHILLVEDDITSAMLIKALLEDFPVKITHVVTGIEAVKA
                     CKKDESIDLVLMDMRLPEMDGFEATRLIKQYNPQMPVIAQTAHAFSEDKGKCMAAGCD
                     GYLTKPMQQEDLINAILKCWQVS"
     gene            18366..18713
                     /locus_tag="PK252_03535"
     CDS             18366..18713
                     /locus_tag="PK252_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT13814.1"
                     /translation="MKTSILVEKLGANVVCGKEHLETKEIEYAFASDLMSDVLTINAK
                     NILLITGMANIQTIRTAEMADIHTIVLVRNKKATPEMIELAMQNDMIIAESSNSMFKT
                     SGILYNMGLKPVY"
     gene            18719..19141
                     /locus_tag="PK252_03540"
     CDS             18719..19141
                     /locus_tag="PK252_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOT13815.1"
                     /translation="MQLKFHIEGGNFTKAGFASSEVKKYLRKINISNETIKRIVIAMY
                     EAEVNVVAHAYEGEILVNIDSKKVDISVIDKGPGIEDIELAMKEGFSTASQKVREMGF
                     GAGMGLSNIKRNANEFIIKSQPGEGTSLNIIVYLNNVN"
     gene            19143..20504
                     /locus_tag="PK252_03545"
     CDS             19143..20504
                     /locus_tag="PK252_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT13816.1"
                     /translation="MEQTHFHHALKVMPELCTACSHCMRSCPTEAIRIRNGKANITGN
                     KCIDCGECFRVCPSNAIYVEQDDFNAIFNYKYRVAIVPVVLTAQLSLLYSMDEIFSAF
                     TNIGFTHIYEVENSAHILRQFQQEYIAQNKEFKPLISCFCPAVVRMIQVKFPSLTPHL
                     ITLKQPLDYAAMYCRKRLEDAGISEKEAGIFYITPCAAKIAAVKSPVGEEKSAVTGVI
                     NMDLLYNRIRRNITNNPGAKQNDIIPFERPSKIGYTWPLTKGEAKNINGRTVAIDGLK
                     NALDFLEKIENEETSGFDFIEIRACDESCAGGILSSTNRFMVVEELEKEANAAKNEPK
                     NIDQYIGFLHANKDIQAVEPRSIMQLDNDTTKALEKVQKIQKLMCFLPGFDCGVCGAP
                     SCNALAEDIVQGHAHISHCLFMQRVMEQKHKLNPDHAYHIIEQIWGEKRLEKNCNKKG
                     AKQ"
     gene            20501..20842
                     /locus_tag="PK252_03550"
     CDS             20501..20842
                     /locus_tag="PK252_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT13817.1"
                     /translation="MNVKDIASLINAKVVSGQNGLDKEVTGAYTSDLLSNVMANIEEN
                     QIWITLQTHKNIIAIASLKDIAAIILVQDLLPSEECAEQSNIEGIPILSTSLNAYQIS
                     GKLFEILKNNA"
     gene            20904..21644
                     /locus_tag="PK252_03555"
     CDS             20904..21644
                     /locus_tag="PK252_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT13818.1"
                     /translation="MNRYKADLHIHTVLSPCANIEMSPVNIVKRALMKGLNIIGITDH
                     NSTLQCATVKELAAPHGIMVLCGAEVTSKEEIHCLAFFEDDESLAEFQKYLMLHQLKQ
                     PNTNNSFGYQVVVDKDNNITAEIDNYLGMAMDVGYLQIQEIVHQLNGLFIPAHIDRVR
                     FSLMSQLGFVPNDINADALEIFNRTSISRFMKENGYLTHYTFIRNSDAHHLDDIGQYY
                     SWFIMNELSFSEIRMALKYKNGRLVTID"
     gene            21648..22205
                     /locus_tag="PK252_03560"
     CDS             21648..22205
                     /locus_tag="PK252_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT13819.1"
                     /translation="MEEIALHILDIVQNSIVACANLISIEVEEDYANDRLIIIIVDNG
                     KGIEKHLLDKVADPFYTKRTTRKVGLGLSLLKQSAEMAGGSFSIESEINKGTTVKAIY
                     QHSHIDRQPLGDIPQTISILVASNPDINFTYKHKINNNSYYFDTRQIKEVLEEVPITS
                     PEVRVFIHQMLKQNIDMLNRQFKEI"
     gene            22228..22626
                     /locus_tag="PK252_03565"
     CDS             22228..22626
                     /locus_tag="PK252_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT13820.1"
                     /translation="MEKVKTLDDLKKLKEQLKSTIELREKSNNPESHVQVKVAMATCG
                     IASGAREIMSFFMDELRKRNIDAIVSQTGCMGYCYAEPTIELTIPGHEPVVYGNVTKA
                     KADEIIEKHIKNGILVDGIIPVTYQNINKK"
     gene            22745..24538
                     /locus_tag="PK252_03570"
     CDS             22745..24538
                     /locus_tag="PK252_03570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT13821.1"
                     /translation="MMKYKMHVLVCGGTGCKSSDSDMIAHNLRTAIDQKGLTEEVQVV
                     MSGCFGFCEKGPIVKILPDNTFYVHVTPSDTTEIVEEHLVKGRRVERLLYKDPQRDEI
                     ISDSKHMGFYKKQVRIALRNCGFIDPESINEAIARDAYQALGKVLKEMTPQQVAEEIK
                     KSGLRGRGGAGFPTGLKWEIASKSPGPEKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYCIGASNGLIYIRAEYPLAIERLKIAINQAREYGLLGKNIFGTDFSFDIELK
                     YGAGAFVCGEETALIHSMEGQRGEPTFKPPFPAVAGYKMKPTNVNNVETYANIPVIIM
                     NGAEWFASYGTEKSKGTKVFALAGKINNVGLIEVPMGTKLKDVIYDIGGGIKGNKKFK
                     AVQIGGPSGGFLTEKHLETEIDYESLTAAGSMMGSGGMIVMDDSDCMVAMAKFYLDFT
                     VEESCGKCSPCRIGNKRLNEILEKIAQGKGTIEDLELLRNLGNVIKDTSLCMLGGSSP
                     NPVLSTLDHFYDEYLAHVKDHKCPAGQCKALMQYIILPENCVGCTACARNCPVNAITG
                     ERKKPHVIDQAICIKCGACVEKCKFNAVVVQ"
     gene            24557..26341
                     /locus_tag="PK252_03575"
     CDS             24557..26341
                     /locus_tag="PK252_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT13822.1"
                     /translation="MIKITIDHKPIEVEKGTTVLKAAQQAGIHIPTLCYMHLEDLNIE
                     HKPGGCRICVVEVKGRKNLAPACATECLDGMEINTHTIRVLNARKTVMELILSDHPTD
                     CLVCAKSGNCDLQSMAHQLGIRKIHFEGEQSTYREDTSPSIIRDMDKCIMCRRCETMC
                     NNFQTVGALSAVNRGFMAVVAPAFEQNLDHSVCTYCGQCVAVCPTGALTEVDHTNSVI
                     RALADPTKTVIVQTAPAVRAALGEEFGLEAGTLVTGKLVSALRQLGFDYVFDTDWAAD
                     LTIMEEGTELLDRLARHLNGDKTVALPILTSCCPAWVKFFEHQFPDMIDIPSSARSPQ
                     QMFGAIAKTYFADKIKVDRKNLVVVSIMPCLAKKYECSREELGHDGNPDVDFSISTRE
                     LANLIKQSNIDFTALPDQDFDNPLGESTGAAVIFGTTGGVIEAAVRTAYELHTKKKLP
                     KIDFHELRGLDGIRQATIDFDGLPINIGIAHGLGNARKLLEDIKAGKSKFHAIEIMAC
                     PGGCIGGGGQPLHHGNSKVIKARHEAIYKEDALKPIRKSHENPYITKIYEEFLGKPMS
                     EKSHHLLHTIYFDRSKKNVIISDEHQAD"
     gene            26377..26865
                     /gene="nuoE"
                     /locus_tag="PK252_03580"
     CDS             26377..26865
                     /gene="nuoE"
                     /locus_tag="PK252_03580"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT13823.1"
                     /translation="MSSIKIQLKQSQIDKIAEICKQFNNDEGELINVLHKTQHTFGYL
                     PAEVQEVIARELKIPVAKVYGVVTFYSFFSMLPKGKNPISICMGTACYVRGAEKVLDE
                     LKKELNIKVGETTPDGKFSLSCLRCVGACGLAPVLMIGEKTYGRVSPEGIKDILKEYE
                     NQ"
     gene            26998..27747
                     /gene="tpiA"
                     /locus_tag="PK252_03585"
     CDS             26998..27747
                     /gene="tpiA"
                     /locus_tag="PK252_03585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT13824.1"
                     /translation="MRKKIVAGNWKMNTTLQEGVKLAQEVEALAASVSNDALIIVCTP
                     FTHLTEVKKSIKKVKLGAENCAAEVSGAYTGEVSASMVASTGAEYVIIGHSERRAYYH
                     ETDEILAKKVKLALANNLTPIFCIGELLSEREAGKHFDVVKSQLEKGLFDLSAEDFGK
                     LVIAYEPVWAIGTGKTATSAQAQEIHKVIREAIAAKYGKTVADNTSILYGGSCNDKNA
                     AELFANPDVDGGLIGGAALKAESFITIAKSF"
     gene            27837..28187
                     /locus_tag="PK252_03590"
     CDS             27837..28187
                     /locus_tag="PK252_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13825.1"
                     /translation="MAELAAPKVILKKKQIPVIIDFCLDESIEFSVRQQAFPETDWEV
                     ELKFKDFKTALLTGMFLRENKFEIEGIDAQRYKKSATPAKKTEEKTDAPKADPQKAEK
                     NKPETDEFQAPSFL"
     gene            28375..31224
                     /locus_tag="PK252_03595"
     CDS             28375..31224
                     /locus_tag="PK252_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT13826.1"
                     /translation="MIIKAFCFLLVTYPAFSTSFSQNLKELFLDAEYSLEIMEFEPAL
                     KNYLILLKKDPENCNLNYRAGNCCLQITERKKEALTYLEKAIKQISVNYKAGSFNEKT
                     APPDAFMLYGIALLNNNKINEAREAFKKYKDYLQPKEVAELDAVNQQLKYCDNAEIFS
                     QTSLNISVTPLPISFNQAKPKLRMVTNAEKSQMVFLADKGGKPGIFYTYLTKGQWSTP
                     TEITKDLEIDPNIDLFNICSMSADGRWLYLEITDEFDSRIYVAKLIGTKWSECTPLNR
                     KINSSNDETHAAESPDGKELYITSNRKGTIGNLDIYVSRLKTNGEWDTPTNLGNTINT
                     YFNEETPFVSNDNQHLFFSSQGHSSMGGYDIFVSDRLPNGEWGEPRNIGYPINTTSDD
                     LFYFPLDSNKALYQLDHSAPFAISQVEILPKVNNIINTDTLNLANADTVKTDTVKQVT
                     HVIETFDTTSKPIVEKDTTPIAPVVAEPVISQLKGKISFSDDSKSFDIINAFIMDKNN
                     NKKSLSIRSDGTFDQTISPGQYKISFEANEYQKVIKNILITKGQPAVLDIMMRPEVIK
                     EQYVSIRSILFNYNSVLLDNESKTMLERLIRIMRDYPSLTIEIIGHADSKGNPEYNRK
                     ISLDRARAVADYFVNQGIDENRFTLFGFGDALAIAIDVNPDGSDNPAGRRFNRRVELR
                     LANYSNLKIVNTELEIPENLRIKETFEYYICLKESPTMVSKDEYISKADTSMIKINNI
                     GQGYIVTLNPPMSKAEAIKKLEEINNTFPEAYIIPDNYFTKVKKVSTVGSYRYASSGN
                     FTIQLLATKRKMKTTAFKPYTDVREYKGMDGLLRYTYGLFSSWDNAYPTLLKVRKQGY
                     NDAFIAPMAKFTGNLLDKKLNGYTIQLWTGFTPKDTSIFRNVGPIKVNKTSDNVYRYS
                     TGTFETFPEAKARLKQLQKLGYSNALIKKQSDMEK"
     gene            31270..31818
                     /locus_tag="PK252_03600"
     CDS             31270..31818
                     /locus_tag="PK252_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT13827.1"
                     /translation="MEQYFLDLVKKRQSVRAYKTDAVEPEKIARCIEAARLSPSACNA
                     QPWKFIVVDDQKIKNQIADLTTTKMVPMNHFTKQAPVHVVLVMENPNLTSFVGSVIRD
                     KPFTLIDIGIAAVQFCLQASAEGLGTCMIGWFNEKKVKKLLNIPSSKRAVLVITLGYP
                     EDDTIREKIRKPVEQIYSRNSY"
     gene            complement(31815..32810)
                     /locus_tag="PK252_03605"
     CDS             complement(31815..32810)
                     /locus_tag="PK252_03605"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOT13828.1"
                     /translation="MGIYSIFGQKKAVASVGIMFYNVENLFDTEDDPEKNDNDFLPTS
                     KHYWTIERYKKKLDNISKVILSVNVDMPVVVGLAEVENERVLDDLVKNTGLSKFNYKI
                     IHKESDDPRGIDAALLYRPDLFKPLSSKFIKVIGKKGRGQLRTRDIVYTKGVLAKTDT
                     VHIFINHWPSRLGGELKSEQKRMFAAAVMRVQVDSILKQNSRSSIVVVGDFNDDPDNM
                     SIKNTLRARGKAEAVNKGDLVNLSAEWVGNINRIGTLKYKGKWNVFDQVIVSRFLADK
                     TTGLCVSKAEIAALPFILQEDERHGGKKPLETYDGMKYTGGYSDHLPVLLKLQVH"
     gene            complement(32918..34399)
                     /locus_tag="PK252_03610"
     CDS             complement(32918..34399)
                     /locus_tag="PK252_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13829.1"
                     /translation="MVRRLFQLISVVALLLLVFSCEDDTVYTGSDARLEFSTDTVMFD
                     TVFSTLGSTTKRLKVYNPLNQPIEIKSVKVAGGAQSPFIININGLADTASNFRIEAHD
                     SMYIFVQVKVDPTGQNSPLVLEDSLVFNTNNNLQKVKLLAWGQDIHLLKDSIIGTQTW
                     TNDKPYVIYKALLVDSLATLTINPGTKIYMHRGVWFGVKGTMIVNGTKENPVVFRGDR
                     MDVSNYAPPVPYDEIPGQWEEIRFMNGSKGKLNYAIIRNGQFGIVAGVLNKEEKAELE
                     LSNCKIYNHAIVGLFAINAKVTANNSLIANAGYASFMCSPGGEYEFYHCTFANYPSFG
                     ISGGSAVVLMNYAVESDTTSSNIYAKKYYYENLTKAYFGNCLIFSENVKSLVLEFSDK
                     KAANYLFDHCLIKYPKNGLNLSDETKFLTTKVSDKAEPGFVKVDVINYKYDFQLTRTS
                     IAREIGSIDIANLYPVDLIENSRLVDEMPDAGCYEYVAPAENK"
     gene            34529..36166
                     /locus_tag="PK252_03615"
     CDS             34529..36166
                     /locus_tag="PK252_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT13830.1"
                     /translation="MFQALKHPFFLLFAILLTGCNTFSPNHNNIIRYNESKGIPTLDP
                     AYARNYPAILAVVQLYNGLLQLDDSLNIKPCIAKKYVISENGTVYTFTLRNDVFFHDS
                     PLFAQGKGRKVTASDFIYSFLRVMNKTTASPGSWVFNGIDTTKANHGFEATNDTTLKI
                     YLKVPNASFAGILTNSYCFVVPHEVVDYYKKDFGTHPVGTGAFKLKLWQDGEKLVLLK
                     NPSYFETDKSGKHLPYLDGISISFVADKQSEFMMFIKGNIDFIIGVNASFKDELINME
                     GNIRKEYKNRFYMLKCPYLNTEYLGFQLDNNLPAFKNSPLHNLYIRQAINMAIDRKNM
                     IQYLRNNLGYPAEYGFTPNGMPDFEKMKTKGFYHNPDSAMKLLAKAGFPQGAGLKPFK
                     LFTSSDYLDLCEFIQHELEKINITCNIEVTNGLAFRDMIANNKMEMFRASWIADYPDP
                     ENYLSLFYSKNFSPAGSNYTHFSDESYDLLYEKALKTVNLNERYLLYRQMDSLIIANA
                     AIVPLFYDVAVKIAHKKIQGLSNNPLNLIQLKYASKQ"
     gene            36605..37411
                     /locus_tag="PK252_03620"
     CDS             36605..37411
                     /locus_tag="PK252_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT13831.1"
                     /translation="MYKKVWTKCHEAICSINFFSEKGIRFDSVTGFKYGRYIITGSQV
                     YKMNKAREVQITFFQSDGLSTKAQVKITWKDFSSRLVKGLNENVSDFTFIDMNFPQFD
                     NIPSLQLRNGGVVAIGTEVALIGHQFDHNNLCLKKGIVSSNVKKNGLNYIQIDSSLEK
                     GYAGAPVIIPDTGEVAGLLGHRLNAISESRKQMMDIINNNLELLKETENKIHFNNIDP
                     IQVLIANQNQIKRLAQELYKTASINYGLAIEISQVAEYIDIEQIENVEYR"
     gene            complement(37597..38631)
                     /locus_tag="PK252_03625"
     CDS             complement(37597..38631)
                     /locus_tag="PK252_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT13832.1"
                     /translation="MRKIINILITLLTLAATSQAQQIQKVCEKGTAMRYNVSGPDGML
                     FHWRAEGGVFATADTLNDTIYVSWNTSGFRKLSVYGELNGCFTNWKELQVEINPTPKN
                     IFDNITDDTLKLCDGEQRTIEVVEGFKAVEWSDHSTNSWFLVNSAQTVWAKVTNEYNC
                     SKQDTIVVSYHPLPVVNLGNDTILCKGSLRLNAYNEGATYQWNLNGPDGFPLNTEEIE
                     ITPGERLYSVAVTNVYGCTGYDTIRILACNVSFDASKILTVITPGNDGHNDVWDIPGL
                     NLEFPNATVEIYDRWGRIVFKSAKGYPVPWDGKNQQSGKIAAMDSYFYIIKLNDKRNQ
                     AFYGTITVIR"
     gene            complement(38635..39318)
                     /locus_tag="PK252_03630"
     CDS             complement(38635..39318)
                     /locus_tag="PK252_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13833.1"
                     /translation="MRNNKLHIIIWLVMLTLSFTGVVAQEKHLTIKEGNKTLRVGSAT
                     ADNFMWILDNDTIEEVKTDSLPKKWAVGNYQLSVIPFDGVCSGEPFNAIIEAKEDPTI
                     GGGQVHFSQESVNICPPSDVKPVTGYLELPVYFDGDVLKDGESYLFKYRIDNGPVQSS
                     FPYNKYNGVLTVNIQGLSVGTHYVRITMLYYKEGYSYQVDYSTSPKIPTMRIDIQDVP
                     SIGDINYEK"
     gene            complement(39386..40060)
                     /locus_tag="PK252_03635"
     CDS             complement(39386..40060)
                     /locus_tag="PK252_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13834.1"
                     /translation="MKTRILFSAIAVFVFASGLMAQIEVLQNTNRDFSVNLTNGITDP
                     VTYTWNASGAGITNSAAVNAITANTVNVTFGAAPSTGNLTVYGTANGCSGDNRTATIN
                     VVTTLTFGATFADASQTVCPQTTTNPTGGDAAATVVNFTGGNVTGISYTIDNGAAVSI
                     SFEEAKTSCSLDAATAFTNAQAGAHTIRIISITGESGTTSLFSAGTEPTHTINVTTAP
                     EISNIF"
     gene            complement(40096..40737)
                     /locus_tag="PK252_03640"
     CDS             complement(40096..40737)
                     /locus_tag="PK252_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13835.1"
                     /translation="MAVAAIAFINQSANAQQTVQTGASVTYKTGGTGSSTFHWDVSGT
                     VQSSNSDTMRYTWSTVGTFTVNVYGISSNSCNGPTRSLNVTVSNTLPVTLTMGAISSI
                     CPYQDTNQPTGGDPISTLTFTGATVGQTYEVYYSIYNGATFVSSNSVSGTLTTLTPTV
                     TFDESFTNTSGSNITREIRITSYTVNGVNYVPSTLPSQNVTIYSSPETSPISY"
     gene            complement(40793..>41514)
                     /locus_tag="PK252_03645"
     CDS             complement(40793..>41514)
                     /locus_tag="PK252_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13836.1"
                     /translation="KKLMLNFLLHIVQNVTTRVAAILFMATMLVGHAYGQNHTVVAGS
                     SYNYTTSQNASTGSSTFNWNITGGSNGTNFSYSTADHDNSIAVSWLQSGTYTVSVQEV
                     SQEGCASGNITNYTVAVVPNNAHIAFASASSDDCSGATGTDLTLNLTFSGNIVYPVVI
                     TYTLTVGATTTSHSRTINSGTSIDLGSEDDILVNSTTSDIPKTITITNATSHGAIMTI
                     DAQNTHIYTVLGTPVTEPISY"
     assembly_gap    41515..41614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41615..42488)
                     /locus_tag="PK252_03650"
     CDS             complement(<41615..42488)
                     /locus_tag="PK252_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13837.1"
                     /translation="MVYKFFSTLKIKIIVVLTMVFYYLPFNAQTTYEAASAGDWSSAA
                     TWNGGNIPPIVNSGHGVLIRMQGGDKNIDLNSNITVTDGNVYNFEINSGVLSIYGDVS
                     INGNCAMTTQGSGQIKIYGNLNLANANIACSKLIEVFGNVNMTGAGTLNINGGPFIVH
                     GNYSSSGDINITPDGVIAVQGTFEGHLVSYNGDGNLYVGTNSYNLIAPVPSQAVCNHA
                     TYTGPNTSTDCPVGDFVDFSVRCPDIYGTYFGSTTLTSYTVSGTNYCNGTGIVILAGS
                     TSGIVYQLYRNGVYT"
     gene            complement(42690..>44456)
                     /locus_tag="PK252_03655"
     CDS             complement(42690..>44456)
                     /locus_tag="PK252_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13838.1"
                     /translation="VQNGSSNVFNTNALTLNPSSVDVVVTNNNCSRLSSALSITVNPL
                     PDDAGIINGSTAVCTNTLNEPYSVAAIANATSYNWTYSGSGVTINNSTTASPNINFSA
                     SATSGTLTVRGVNACGNGITSSQSIVVSNTPAPATTGSVSGPASVCRGGNYTFTVAST
                     TNATGYIWEFSGTGATVHGTATPVNAYLTSAAPGVDGTISIDFSATATSGTLTVRATN
                     GCSTPAPASSYSITVNPTPTVSIPATYSGCSGTNIALLATVAGGTSPYTHQWTEAAAY
                     GGTFSAATSASTNYNNNTGGTYTLNYVVTDNNTCQASGSTSVTISQAPVAEAGDNIPS
                     CSGNGSISMTGATASGNYSAITWSGAGGTWTQHISNPALAIFQPSATSGTSTVTLTLT
                     GAGGCTNATDTRTLTWSATPAIPSTVSQSFCSGATVADLTGTVDTGCTIDWYSSATGG
                     TPLAAGTALTTGTYYAEARNTATNCISSGRTITSVTVNNAPVIIMGGVLSVCGTNDLN
                     VTGVNITNYSALNWSVVSGGGAIINGANTIVPVYRAASNTAASVTLQLQITALPGCTN
                     PANQTKAINVYVTPTTNGMTHD"
     assembly_gap    44457..44466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<44467..53444)
                     /locus_tag="PK252_03660"
     CDS             complement(<44467..53444)
                     /locus_tag="PK252_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13839.1"
                     /translation="MYRVNSKGKAFRVILFLFIAFFFSAFDAVSQSAGDYRTRNNGNW
                     NGANVWQIRNAANTAWNNTANYPGVDNSLPSIYIQNNVTLNVTPAYHVNNVIFNSNNT
                     ITISNGQTLYVDGSISGTGTISVAGILYVAGNIGTGITVNGNGTIYIEGAFTPASYST
                     TSGTVNYSGLSDQTVSVYSYNNLTVSGSGVKTIANNITISGNLNVSGTATLASDIYQI
                     SRAAAGGSFTLGAGTALTLGRTTNATAVIFPTNFSTVTLDATSRVAYQSAADQTIATY
                     NYGILEIGGGATKALANHTTLNGGLILTNGILNLGNYNLIVGGKVTGGSLTSFVNQSG
                     LGRFIKRGSTVDELVSIFPIGMGTRYVPVSLNSLSGTVAPNSTITVATANSQVVPGIP
                     GSNPLNRQWTTTSTGISGPVLANFSFAYDNNDRTGGATAYDLFVNYMGSWIVPSGLSQ
                     SGVNPMASVSSPFIDATWSGSVSGKRIFYSLKSGDWDDPSVWTLDPSGSQPNNPNGFT
                     PATSTTSMYDEVVILSGRTITVSSGAKVNAKLTVNGVLDLRDVAGHSFTDIEGSGKIR
                     IAGNNFPSGNATNFITAGQGEGTVEFYGTSRDITLATTYYNVEVNMGTNTLTLLNNLT
                     VNGSLLVKSGTLSINNATATNNLNIIVRKDITVNTAGNIVTGSANARHQLDLYGNLKN
                     HGHLAFTNRTAPIVDTYYTTQATDGIVDLNCLNANADQSILCDGPSIFYRIKIDKGTD
                     DTYILNIDATAPTNFYLLGYTNQGHPNINQLITNDNALALIKGTVRIGDGVTISSLSY
                     SGNYNISTAAQLWVDGGTVLENIGNAIVPYGKIRITSGLLESKVASGITVRESGLILV
                     DGGTVNVNQIRTSVLGAAHLGGYIQTGGEVNVGGTPYTSDYYTFCLTYNNSIFNMSGG
                     TLRVNNASAKGTIFINSDPENIKVTGGTVIIEASYARNPSINSRAPFWNLEFRNTSGA
                     QRDFIINEGVDVGTTDVDLAAQPLKVLNDFRIWGTESGGAGYPVIRFTPVTSATNVND
                     VYIGGSFYLENGAQYIPILGGTAPYNSIANQPTTSNTTYFNQTAGTSANEELYFGNAT
                     AELEFGKMVVNKTTGNAIRLSAPAARTNGSVLLDVNGNISVLSGTLDQGNYTIRTWGN
                     IYNYDRMGTNTPTTPSSAQIQFADKTGLTINTSNKAIFGNVQFNVSPPTIVSLTSDIY
                     IERLQFVKGLIYLKNFNLKVDDLWNLETNLFEDVANSSNLKIVNSGLAAGCNSMIFTD
                     GNAGDGGLTLRIYEETLAENQVNRLNNRAPVTFPVGFTPNGGTTLYFRPAQMVVSHLA
                     AGDDGYVTVRPVRGALPTSNLTGGEVLQHYWRVSRSGFNTNPTVSYRFYYRNNTGVAN
                     VDLLNNENNFLPGKVLDESPYTRAFEPLTDNDIVKNFGAGNNSRMITFNGTSTNGLFT
                     NAAVGFVAENTNFTAGVSTRFSGSVLIYYTRDHGVNDNDGTQHITNREPRWQDNLTWT
                     RSDILNPLYSPHDSRQPAVAVGDYPKAGDVAQIGWVPYDDVQKDGNPANGGTATDNID
                     QRGLPHGVWIDNTTHAAAEVVFYKMTDIAGNPVARKYRSNFQFRPTLCINNYNGQLSA
                     KNVRGEGMFWNRDNSDPSYYLMDIGDFAREDSSYVLYENFETPFTYQNTAPLFPNVMI
                     SNDGWGNNNHDITFARDINTTGDFEILGDINLVLSSGATGNINIGRTLKFFESAGVQG
                     NRSYGGAEIAYQNSGNERTITVGRGISMKNTSAIINVRNPNGGIALEHTLKVNGDIYI
                     DEKPYADETPAVPNGLQLGNAGSLAHINLYMTGGENATYTITNPTAGNVTNLYRLIID
                     KGSSIASTVQFNCNFNLNGPTSGAGVAKALELKNGLFIMNNAATNINLTTGYDYFSIP
                     STAGLEIRQGTANANGNSGIELDGLLRIAGGTLNMAGGNNPIEYSSSGNSVLQISSGS
                     LTVGAQIRRSPTSNIGTLDYTQSGGTVIVGENSATVNNRGVFEILNPGSSFNMSGGDL
                     YICRQQSNPTIASFYFDPDVNNISTAANIHIGHPSLTPASQTISIYAGDELPKLHVFG
                     FNSPTARLDVVPATITNLLQVDAGATFNANGKDMYLMGDMNVAGTFVPNGNTVYFDGS
                     LTQTITGNGTPLNFYNLTKTASNNLALTANNTPLTILNNLAIQTGTLTDNGNTISVKG
                     DCYNDGTHVYGGLGNGIVMNGVQPQTLTGNGTFGKLTINNSHGVNVPVANQFKITNAL
                     RMQAGIFNIGKNLLDLGLNCTIEQASPFSDINMIETNISFTDNGVRKVFPATASPITF
                     VYPVGCDGKYTPVTLNITANGNSTGSITVKPANEIHPSIIEDTETGVAIVDADNALQY
                     YWTMKTSGISAFSGTAKMKYLPSDIKFTAPYSINDYIPASLQNDGVGNWVKGDWAEVD
                     QINNELSFSFANVGDAELNADFTAGAVDATKNGAIPDNVMNYETLSSGNWQTATVWSP
                     NVTNGPSGAITKINAGHTITISVNGIKEYATNIYGRLNLGVTTGHRHGIIGGTGTMYL
                     EQGSISAGVYDEFFSTAGGTIEFGGADNYDFLGNILSVNHLVLSGSGDKRFPNNNLEI
                     LGDFTINGTALLYAVTYDNRKIYLHKDLIRISGNFEAGANSYMVFNGSIPQNITGSFT
                     GTNAFKGLEIANINGLTLNNDVEINGTLKLTAGLINTGSNNIRINYSGTVSPAKGLSN
                     SFVNGTLTKVMINGGSFTFPIGNNNGNLGAMALLAVNGPSGYRDWSASYTYDNAHTAG
                     YDTSSRLSPVVKVSGNEYWSVFAPSTSSSGKVRLYLDGSSDIAGAISNINNLRIVGWN
                     SGSSRWEVVGAGSIITGSATSGYITTKNAVNFGTYSVFTLASVANISNCTATITSSDA
                     IVCQNATANVVVALSGTGPWVLRYSLNGVTQPDANVTSSPYTINVPTATANTYTYVLQ
                     SVSQGGLPGTLVGNIDAVIKVNALPNATISSSNTAVCEGAAITFTANAG"
     gene            54220..54611
                     /gene="ssrA"
                     /locus_tag="PK252_03665"
     tmRNA           54220..54611
                     /gene="ssrA"
                     /locus_tag="PK252_03665"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            54759..55358
                     /locus_tag="PK252_03670"
     CDS             54759..55358
                     /locus_tag="PK252_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT13840.1"
                     /translation="MRRIIMDDEPEMEPQSKKSASMKIIDLKFLEQRKIFLWGVVDDD
                     SAQDIVSKLLYLESVKPGEKITFFINSPGGMVTSGFSILDTMNLISSPVSTVCMGLAA
                     SMGSILLSAGEKGERYIFPLGEVMIHQPSVGLLQGTAADIEIQTRQLLKTKEIGAEIL
                     AKNCNQSVKQIYKDFDRDYWMNAQESINYGIVDKIYEMK"
     gene            55465..56163
                     /locus_tag="PK252_03675"
     CDS             55465..56163
                     /locus_tag="PK252_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13841.1"
                     /translation="MFTWKTKGGYRIVQLIGGRSNVFLVFYGTKKIMVDTSTKWNWNK
                     LDKRLRLYAINEIDYLVLTHSHYDHAENARRIREKYHATVIVHKKESHFLLGKENIVP
                     AGTNFFTRFLASFFGKWFFSLRKVTCPVDIVVDSTFSFHDFGFKAYLIHTPGHSEGSM
                     SLIVDDEVAIVGDAMFGVFRWTVFPPFANNPRQLVDSWAPLLNTNCQVFLPSHGNANT
                     RDMVLKDYEKRKIA"
     gene            56278..56712
                     /locus_tag="PK252_03680"
     CDS             56278..56712
                     /locus_tag="PK252_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT13842.1"
                     /translation="MADLVFSVEGKAESQTRITAKARQFSIVVDEPPTLGGQDLGANP
                     VEYLLASYAGCVNVMAYLIAAELNIKLDKLTIKVDGNINPNRLFGKSFSERAGFKQIN
                     LTLEPKTDASPELISKWIKEIENRCPVNDNLSNPTPVSIKLS"
     gene            complement(56982..58271)
                     /locus_tag="PK252_03685"
     CDS             complement(56982..58271)
                     /locus_tag="PK252_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOT13843.1"
                     /translation="MNKQSRETICVQGGWQPQKGEPRVLPIYQSTTFKYETSEQMAKL
                     FDLEEAGYFYTRLQNPTNDAVASKIAALEGGVGAMLTSSGQAANFYAIFNICSAGDYF
                     VSTSAIYGGTFNLFSVTMKKLGIEVTFVNQDDSEEDIVKAFRPNTKALFGETIANPGM
                     AVLDIEKMAKIAHKMGVPLIVDNTFATPINCRPFEWGADIVVHSTTKYLDGHASTVGG
                     AIVDSGKFDWDANKDKFPGLTTPDDSYHGLIYTKSFGNKAYITKATVQLMRDLGSIQS
                     PNHAFLINLGLETLHLRMARHCENAQKVAEYLQSNDKVAWVHYCGLPGDKYYQLGLKY
                     LPNGSCGVLTFGLKGGRDNSIRFMDSLQLAAIVTHVADARTSVLHPASHTHRQLTDEQ
                     LIAAGVAPDLIRFSVGIENANDIIADIEQALKKAYNL"
     gene            complement(58679..59047)
                     /locus_tag="PK252_03690"
     CDS             complement(58679..59047)
                     /locus_tag="PK252_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13844.1"
                     /translation="MPRIRKILEDKVAKPAPATKKIAKFKNIAAKKTENPVNTIQGKT
                     ENPVDLKLPKPTKRKYIRKEKDFISFLKQEIENRDFSLEQIEKIREILENIKEKEIQK
                     LEENIRKNQERLEALKKLKG"
     gene            complement(59267..59851)
                     /locus_tag="PK252_03695"
     CDS             complement(59267..59851)
                     /locus_tag="PK252_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13845.1"
                     /translation="MKHVKIFIFFFHALLLVNCNNCYDSARAKYINKTFYNNSCSSDF
                     VVFTAVDTKTNTAKEICCESNALYSAYMSEVISLNKSVSQIKADEIAKKSFDSIVNNK
                     SCNFVFHFNKAQSLMMIGFYEFNTDSVKFYSNKNTKRIIKAIKANYKPENLLFLKDFA
                     RQENIYQIFLLNKNGIYCGRDCFSGIVMIKKVID"
     gene            complement(60134..61486)
                     /gene="gdhA"
                     /locus_tag="PK252_03700"
     CDS             complement(60134..61486)
                     /gene="gdhA"
                     /locus_tag="PK252_03700"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT13846.1"
                     /translation="MAKTDAALNEFMAKIIAKNPGESEFHQAVKEVAESLMDFIEENP
                     KYKAAKILERIAEPERVIIFRVPWLDDAGNFQINRGFRVQMNSAIGPYKGGLRLHPTV
                     NLGVLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEIMRFCQSFMTELCKHIG
                     ADTDVPAGDIGVGGREIGFMYGQYKRLRNEFTGVLTGKGLGWGGSLIRPEATGYGTVY
                     FVREMMKMRKMDMKGKTVVISGSGNVAQYAVEKAMQYGAKVVTLSDSNGFIHDPEGFN
                     EEKLAFVMDLKNVKRGRIKEYAAKYKSATYHEGKTPWGVKCDIALPCATQNELNEEDA
                     KTLIKNGCIAVAEGANMPSTPEAIEVFLKSKIMYGPGKAANAGGVATSGLEMTQNSMR
                     LPWTREEVDAKLDQIMCNIHATCVKYGSEKDGYVNYVKGANIGGFIKVADAMIAQGLV
                     "
     gene            complement(61620..62276)
                     /locus_tag="PK252_03705"
     CDS             complement(61620..62276)
                     /locus_tag="PK252_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT13847.1"
                     /translation="MSISIGLFDEHKIVMEGLSSLFSQNENIKIIFQCSDKAKLLETL
                     KLQPVNILIVNIHVLDTAILNLISQINLTISKTRILVISAVNSDEMVLKTIKAGAKGY
                     LAREADRNEMVEAIYTIRNGFDYYSKSITHLLINKYITRFKSEDKMPKAEIGNLSARQ
                     VEILKLWGENYTNKEIAEKLFISLRTVETHKNHIMQKLNLKTTVDMVKFAIRNNLIEI
                     "
     gene            complement(62300..66892)
                     /locus_tag="PK252_03710"
     CDS             complement(62300..66892)
                     /locus_tag="PK252_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT13848.1"
                     /translation="MATKNISAEGVTEEPSISKIDLQLIHSFEQEYPVNELLKKVCNL
                     IPRAYLQPSEVCARIYYNSSIIDSNNYQDSPCLTKYTFNLPDGTLGAVELHFFGAYAD
                     SFSKAFVAKNAAFLKDVSTLLEGELSKNQLAKLANATHERSKELKGISRAAEVLKRSD
                     TLEESLQNICLFLPEAWQYPAHTAARITFDNMEFKSKKFQDSQWVQRAYFNTPDNKEG
                     KIEIFYLKEFPEADEGPFLSEERQLLDNLALLISGTASEEALQHLLLQNTERLKELRA
                     INQTSEILKQNNNMDDALQKICSILPEAWQFPEHTVARITYKDKVFRSKNFKVTKWKI
                     NQVFETPDNSKGLIEIYYTRAFPEADEGPFLKEERNLLVNISTLIAGSATKDVLRKLQ
                     YENRERLKELNAINQTSAIIAKGESIDDTLRDICAIIPKSWQYPEHTVAAIKFEGNIY
                     TSKNFSETIWGQREHFVTFDNKKGTIEIFYLKEFPEEFEGPFLKEERQLLINLAKLIS
                     GYLNNYKGREIFNKSQVKEIAGHNSEEYRKSLIKNKQPLQLFFNKQILDKYIYLDMMK
                     YKVKDILFVATLYDAFILENEDSFFEQFMGEIYQYSLFSLPRITGVTSAEEALELLDT
                     TSFDLVILMVGIDSNAPIDLSQKIKQVRPNLPIFLLLNQKSNIKYFEELVPTVKSVDK
                     LFVWNGNSQIIFAIVKSIEDSANVENDTKVGLVRVILVIEDSAQYYSKYLQTLYSVVF
                     GQIQQLLPEVEKNEIDKISKMRSRPKILHARNYEDAMYIYNKYKDFLLCVISDVEFER
                     DGKLDKHAGVRFSKYVKANLKNLPILLQSSEVKNQKIAEKIGVSFINKNSESLLTEIK
                     QFITYHLGFGDFVFRDKEGRQLAVAHTLKEFRDILYTIPEESLFLHSEANQFSLWLMS
                     RGEIKLAKTINPFRIKEFESIEVFRQNFIKTINDYKEEKIRGKILTYEETSIIDEKNI
                     YLLANGSLGGKGRGLAFINTLIHNLDFSAFNKEINIRTPVTAIIGVEEFESFMERNHL
                     YDKIFNANISYEEIKHHFLHAHLSHGLMRKLEHFISRINKPIAVRSSSISEDSLSQPF
                     AGVFDTFIVLNNSKNKKINLDHLTKAIKLVFASIYSDHSRSYFKAIHHKVEEERMAVV
                     LQELVGNQYGNYYYPHISGTAQSYNYYPVAHMKPEEGFAVAAVGLGNYVVGGGKSFRF
                     SPKYPKIEIFTRKDLLNSTQVNFLAVDMSKKNINYIEDGEMAPLALLDISVAEKDGTL
                     KHCASVYNPDNDRIETGINSPGPRIVNFANILKYNYIPLAQLIDVLLNTIKEAQGCPV
                     EIEYAVDLTPSANGLPSFYLLQIKPLVGSQFNHNFDFDEINKENTILYTESSLGNGVI
                     NNIRDVIYVDVAKFDRLKTLEMVKEIEHLNNMMQKNDRQYILIGPGRWGTQDQFLGIP
                     VVWGQISNAKVIVEISLSNFPLDSSLGSHFFHNVTSMNIGYFSVHDTSLSEFIQWKIL
                     NKQKVIHKTNYFKHVQFAKPLKIVMNGREKKSVIMF"
     gene            complement(67070..67552)
                     /locus_tag="PK252_03715"
     CDS             complement(67070..67552)
                     /locus_tag="PK252_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT13849.1"
                     /translation="MIEFRTQLVSGDSQAIGELLESTGFFYSYEIDVAKELSDANQEK
                     GAEASGYHFIVAYENNTMLGYCCYGLNPCTQSSYDLYWIAVHKSQMNRGIGRKLMTMA
                     EQAIAKLGGTIVWVETSGRVIYEPTRAFYLAIGYDKVAIMPDFYAPGDDKVVFMKRIK
                     "
BASE COUNT        21876 a        13622 c        11989 g        20140 t
ORIGIN      
        1 tatcgcctta actctttcac cctcgaagcc ttgtaatgtt gcaatatgca aacaatagaa
       61 gctttaggct tctcggccga aatcgaacaa aaaggcgatg aaaaggaatt ttttcttttt
      121 attgcttcgc ctttttcata gaaacttctc tgctaatgac ctaacgtaaa tggcattaaa
      181 taaagcgcac caagcacaaa acttataaaa tgttctagtg cagtatgatg cgcattaacg
      241 caagctcatc aaaagaatta aaagcaaatt ttaacagcta tactgcacag ctgttagcat
      301 ttttattaac tttgaaaagt agccaacatg aattgaaaaa attcatgatt tggctcaacc
      361 acctaataac ctgtgaagct attctaatga ctatgaagta tttattaatg ctgacattaa
      421 cagctatcct tattatcaat ggatgcattc aaaagcaaaa tgataaaata aaaattgggg
      481 ttatgttctc caatttgaaa gaactacggt ttcagaaaga aagctccatc atggagcaat
      541 ttataaaaga gaaaaattat gagtatctcc tgagagatgc agaaaacgac ccgtctaagc
      601 aggaaagaca atttgcagaa ttgaagaatg aaggtataaa agtactgatt atctctgttg
      661 taaataataa cgtcggaaaa tccatcatca aagaagccaa taaaaatggg ataatcacca
      721 tcatgtatga cggtttcatc ggaagtgagt ttttaaatta ctgcattcgt ttctctgaca
      781 aagaagtggg caagtatatg gcaggatacg ccctgaaaaa tgttccggaa ggcaactaca
      841 tgattttagg cggagacgga agaaacgaga atgccccttt aatcagaagc ggcatcatgg
      901 aggttttaaa caccagcatt gctgcttcaa aagtaaagat tgtttacaac aactttatgg
      961 aagaatggag cgcagacgaa gtgtatgctg cagtgaaaaa atactacaaa ctgagtgctg
     1021 aagaacgcgt agatgctatc atactttcca gtgacaatat ggcaacagga gccattaaag
     1081 cccttgaaga attgaaacca acaaaatggc cacttataac tggtcaggat ggttcattaa
     1141 cagcttgttt gaatatttta caaggaaaac aaagcatgtc cgtgttaaaa gattccaaaa
     1201 aaatagccgg gcaaaccgca cttatggcca ttcaatgtgc cgaaggcaaa acgcccgaga
     1261 caaacggctt tactactgca aaaggcaaca caggtgaaat taaaatccct tcctttttta
     1321 tcgacctcga agtagtcgat tctgcaagta tttatggatt aatcaagcgg ggtatttata
     1381 ctaaagaatc cctggggctt gtaaattagc aagctaactt tattataaga ttctttttga
     1441 agaaccctca accaacctgg ttgaaaatga attatttata tctgtgacat gaaaaatgtt
     1501 ttacttttga taaggttgaa atatcttaat attgtcgtaa tataaaggta ttaaaaacaa
     1561 ttatgataaa gtcaagatat ttaattacaa catatattct cagtttaatt ttatcatctg
     1621 tttatgcgca gaaaaatgaa ataaaagatt ctatattttc tgtaaataat aatacatata
     1681 aggctcttgg gttaactact gcttattatg gagcttctat gtatgtgtta agtaaaacct
     1741 ggtataggga caaagattta gtttctttcc atttttataa cgataatagt ggctacctgc
     1801 aagttgataa atttgggcat acatggggct cttatgttta cagttatatc ggttaccatt
     1861 atctaattaa ttctggcttt tccagaaaag aagcattatt ttacggggca acattaggtt
     1921 tcaccttgca agcacctatt gaaattatgg atggaattca tcaaggttat ggattttcat
     1981 taggagatat agctgcaaat gctttaggtt caggattagt aattggtcaa gaactattat
     2041 tcaatgaaca aattattaaa tataaattta gttattggga gtccagttat gctcatgatg
     2101 caaacggtta tttgggagaa acatcattaa accgaatgtt aaaagactac aacggccata
     2161 catattggtt ttcttttccc gtaaataaat ttttcaaaag tcaatttatt ccagaatggt
     2221 taaatatagc tattggatat ggagcaaatg ggatgtttgg tgagtttgaa aacatttctt
     2281 cctacaaagg tgttgatatc ccaaagacaa tcagataccg acagttttta atttcgcttg
     2341 atattgattg gacaaaaatt aagacaagtt caaaaatgtt gaaaataatt tttaagggcc
     2401 ttacttttgt caaattacct tttacaacct ttgaatacaa ttcgatggga aaatccaaac
     2461 tttacctgat ttattattag aaaaacaaag gcaaacaata tgccataaaa aataatgatt
     2521 aaagaaaaat ctcgaacgtt tcactgctgt ccggctttgt ttcttgccac ctcgttttca
     2581 aaatcgacct ctgtccgtaa gtagaagaaa acaactgaag cggctaaata aagaatatat
     2641 caaagagcca tagactataa aaataaaacg atgcaatgaa acgttattcc gaacaaaagg
     2701 gaacaaaact taacaaatac ggaaataacc gagatatgaa aatagaaaaa atcaacgaaa
     2761 acaagaaaca gttccttgac ttactgttgt tggcagacga gtctgaaatt atgattgaca
     2821 agtacttgcc cagtggcgac ttgtttgctt tatatgacga tgatttgaaa agtgtttgcg
     2881 ttgtagcacc cataaatagc gagacctgcg aattgaaaaa tatagcaaca tacgaaaaat
     2941 atcaaggcaa aggatacggc agggctttga tacaattcat ttctgatttc tacaaaaacg
     3001 actacaaaac aatgcttgtg ggaacaggcg aaacaccggc aatcttatcg ttttatgaaa
     3061 gttgtggatt tgaacaatct catcgtgtta aaaacttttt tacagacaat tacgaccacc
     3121 ctatgttcga cggcgatata caacttgttg atatgattta tcttaaaaag agtttataag
     3181 gattcgggct caacctcaaa ggccgctttg tttcgtacca ccattgttag ctttcagtat
     3241 aacgtaataa atttgagcat tttagcattt tttttgtaac ttgcactcga attttaacat
     3301 gatatgattg attctaataa gataaacgat attgtttaca ggattgctac aaaatttaat
     3361 cccgataaaa ttattttatt tggatcttat gcttcaggtt cgcagaatga agatagcgat
     3421 ttagacttat taataattaa agataccgat ctaccaattc aatctcgtgg gttcgacatt
     3481 cgtatgtcat tgattggcac aaagattcca tttgatattt tggtttatac aaatttggaa
     3541 tttgagaaag aaaaaagtaa tagtttttca tttttaaatt ctgcattgaa gaattcaaaa
     3601 acattatatg aacgagcaga ttgaagaaat aaaaggatgg attgaaaagg ccgatcatga
     3661 tttaggatca gcaaaaataa tctagcgtcc cgctacgtcg attaaactct tcggactctg
     3721 gccgtttcag aaaatgctaa aatatagctc ataatcccaa taaattgctt atcccttcag
     3781 gggttgtaac cctctttact ttcccttctt caacttgctt aatgctttgt ttaattcttt
     3841 ctaccatttc ggcattaaaa taaatatcat cctcagtaac aggagttaag gcataagctt
     3901 tatcttttcc tcgttgaaca attatttgtt ctcctttatc ggctttttca aagtacattt
     3961 tttgattttg cctaagttct ctgctgctta ttactaacat attttttagt tttattgtgt
     4021 accaacttgg tacaaagttg ctaattttta cccttatttc tttcgtttcg tttcatttaa
     4081 ttcttttctc aacaaactac gatgaacaag ggtatgcccc ttcttggtct tttattttat
     4141 acccggttga catatttcaa atgtactatt ttttactgta attcgtgaaa cggccagtgg
     4201 ccgaatataa tacgacgtag cagaatgcta cgccgaaccg cttttattgg cagtatagga
     4261 tggacagggg ttggatttta tagtttgcca acacttgaag tattgagaac cgctttttga
     4321 tgacggttat tttcattgtt gaaaaatatt ttttctaaac ttttttgttt ataaacacaa
     4381 catatttgtt ttacagaaac aatacttatt aatttatttt atcaacacat attaacaatt
     4441 atactatcct aaccttgtaa aaaattaatc tttatgttaa ttttgaaaaa aaatataatt
     4501 ttattatgat aagtttctcg gaaaacctta aaagggtcag gactcaaaaa gggatttctc
     4561 aggaagaact ctcaaaaaaa atagatattc atcccgtgca gttttcccgt tacgaaaggg
     4621 cgcaatcggt tccttctatt gacgttgtgc aaaaaattgc taatgccctg gaagtgacta
     4681 ttgatgagct tgtatatggc aaccagaata ataaagccga acaaaatatc aaggacaggg
     4741 aactgttgag catgtttagc aaaattcagt tgctaaatga gaagcaaaag gaaacggtta
     4801 aagatttcct ttctgcctat atcctgaaag tagatttaaa agaaaaacta attgtctaag
     4861 attatgcaaa cactattagt agaagtaaaa gacaataccg ggttaaaaat tttgcaggaa
     4921 cttgaacagg cccatataat ccggctgata caaacagccc aggagaaaat tccccaaagg
     4981 ctttctaaaa ggttacgtgg tgctttctcc aaggaaacag cccagcaaat gaaatctgaa
     5041 ctggaacaaa tgcgcaacga atggcaacaa cgggatattt aattgacacc aacgctgcta
     5101 ttgattacct tggcgaagcc atgccgcaaa gcgggctggc ttttatggac aatattattg
     5161 acaaggaata taatatttcg gtgattagcc ggattgagct ttacgcttat agcaaactta
     5221 ctgagaaaga caaagagacc cttgatattt ttaccggaca ggcttcgagt ttaaatataa
     5281 acgatgaaat tattgaaaaa accatagaac tccggaaaac ccataaaacc aaactccccg
     5341 atgccattat tgctgccacg gctttggttt gcaaccttgc attggttagc cataatgtaa
     5401 aagattttaa aaatattaca ggcctttcgg ttattgaccc atacagcttg taaaaaacta
     5461 aaggacgcac tctcgaacgt ccttagtaat gtgaagtaaa ataaattttt tcttatttaa
     5521 ttacctcgta actctttagc ttttcgcact tctgtaacaa aggggttttc tcctttggga
     5581 aagtatgtat atactgagtc ggggtttata ctactcatac ttatttttct atctcaagct
     5641 tataatcgtt ataggttttg ctagtagatg tgactttgtt agattttacc tcaattttaa
     5701 tacctacagg ctttcccttg tcaagtttgt caaaatgatt gtttattatt tcttgaataa
     5761 cttgttccac agcccccgtt cggcgtagcg tcccgctacg tcgcattata tttggcctct
     5821 ggccatgttt tacgaataag tttcccatcg tgtagttaat atttccactt ccaatataga
     5881 ttcataacct gcatagtttg ttgcacttga atatatgtac tcttccggct ttctaacaat
     5941 acaatccacg ctacgtcgca ttcttctatt agcctctggc taatttacta cattttttga
     6001 tatctactac ttttgcttgt ttactgattt acggccagag gccggttact atgcgacgta
     6061 gcaggatgct acgccgaacc gcttttatcg acaagctacg atgaacgagg gctccggcct
     6121 cagctgctct tatctttaca ggctatagcc tgatgaaata cttacgacta aggcagagcc
     6181 ttagccgaac gagggtactt acttttatca aataaagaca gctaattttg tagaaacaaa
     6241 acaaatgata ttgattaaat aatcatcttc ttgtggcgca aagtcctaaa tagttctcaa
     6301 atattagact tggactacct ctaatggcgc aaggtctgca acttgtgcct cttttattga
     6361 agtttacaac ttcagctatc cagcctgtaa ccttctctaa atagttcaag acttcgactt
     6421 gaactgtatt cttaacctta aaaagctaaa acagttgcaa actgcaagaa tagtaggcac
     6481 aagtcacaga ctctaaaggg acagttgtga agtctctctg tccccaacta cttgtagcct
     6541 catccttaga ccaaacataa ttcaataaaa tcaatgcaaa taaaatttgt tgattttgaa
     6601 attgtttgca atagtttgaa gctaattaaa gtttaaagta tatttaaagg ctctaaaaaa
     6661 caaagaaagg cttccatctc tgaaagcctt tctatttgtg aagtcggggt gaaccgactc
     6721 gaacggtcga ccacttgcac cccatgcaag tacgctagcc aactgcgcca caccccgatt
     6781 atattttaac actctaagta cgctgctctc gcctcaggcg agacgccaca ccccgattat
     6841 gtttggaagc aagtactcgc ctcaggcgga accatgcccc atttctatca ccaaagcttt
     6901 agcaaaggtc attttcgtgc tgcaaaagta atcaacactt cgagaaattt caaattttcg
     6961 acaatttaaa atttatatac atacaaaata agcacaacca aactatacct aagcctgctt
     7021 ctcaatcagg tatttccaga accttttagg taaaagctgt acatgcaact tagggttcaa
     7081 ccgttcacgg atacaaatgg ctgaaaagta gtttttccag agttcttgga atattttttc
     7141 ctcagcggcc ataaattcct ctgcaaaact tccatcggca gtattgactt tactctcccc
     7201 aaacctgatt tcgctggtcg ttttcaaatc gtaataataa ccaaagtttc tccgcgtatc
     7261 gtaaataatc cagcgctggt cggcaaaccg ctcttcaaaa tgctttgtca ctaaaggcaa
     7321 cacgttatat tgcggatcat aagaagcata atagatatcg tctgctgttt tctgaaacct
     7381 cacaaacata ggtactctcc cagcctcttt atttactttt ttagcaatct ttttcatttc
     7441 cagcacatcc gcatcaccaa agttagcttc tatagtgatg tttgaagcaa atacttttct
     7501 tacataatgt aaaataagca tttcaacatt tcgctcttct gaaagaaaag tataataaag
     7561 catctggcat gtaactgccg aaatacgcct gtgcaatccg ttccacactc ttttagcctt
     7621 tgcctcatca gtatttacaa aatattcata gctaaaaaga ttattattac ttgaattatc
     7681 gcgaataata gactgtggct ccagcttttg ctcgtagcat tcgaatataa gggtcagtaa
     7741 accctcaaaa gtaccatcat atttaattgt catcataaag attattctaa attcaaaatc
     7801 attgttgtcc ggtaaaaaca ggtcgccgct atgcggctta gaaacatttg gtgtatttgg
     7861 ttatcctaca aacagttcgc agctacgctg ctattattta gtcgcgtagc gacgttttgt
     7921 ttgtagtaaa attaaacatc gttaaaatta agccccagcg gggcgacctg tttctaatca
     7981 acgatattac taaagtattt attagtatct gttttatttt accgtacaac agtaattcaa
     8041 aataaaattc gtcttacagt aaggtttgca ataataatgt ttcttttttt cgtcaaaaag
     8101 aaaccaaaaa agactggctg tgaaaactca ctccgcctat cggcttcgct caaacagttc
     8161 acagccaaat aaggaatttt ctttttgtct gcttcgcagc cttcgattca aagctaattc
     8221 caatactggc cgtcataaaa gcgcgctctc gcctgaggcg agatcgtttc cttttttcat
     8281 caaggaaaaa aggataggat agaatataat aaacattcat aattaattag caaaatgagc
     8341 atcagtaaat agttccaact gtttgggcaa attttcgtta gcttttttcg tcgatttttc
     8401 ggtcaataac actttcaatt tgtcgggcgt taattcattt accgttggcg aagctagttc
     8461 atggcaagta atgaagtact ttgcccgttt catcaccact cccattttct gcaactgcaa
     8521 agagttgatt ttgccatacc tgcgcgacat aacaatcatc ttggcagagt ttacgcccac
     8581 tccgggaatc cgtaaaatca tctcatacgg ggccttattg atatcaatgg gaaaaatcca
     8641 cggattgcga agcgcataag ccagtttggg gtcgatatcg aggtcaaggt caggatattt
     8701 ctcgtttaca atttcatcaa ccctgaaatt gtagaaccta agcaaccaat ctgactgata
     8761 caacctgtgt tccctgacta atggaggctg cactattgca ggcaatcggc tatcgtggtt
     8821 gtttattggg atatacccag aataatacac acgcttaagt tgctgttgct gataaagcga
     8881 tgaagccaaa tcaagaatct gcttatcact atcaggtgta gccccaacga taagctgtgt
     8941 actttgtccc ccgggcgcaa aacgtggtgc agacttgtat ttcatcttct cctccttgct
     9001 tacaaggatg ttttgcctga ttgcgttcat aggcatgaag attcctttgt aatctttctc
     9061 tggggcaagc aatttcaaat tttgctccgt tggaatttca atattgacgc tcaacctatc
     9121 tgcatacaat ccggcttccc ttatcagttc atcacttgca cccgggatac ttttcagatg
     9181 gatatacccg ttgaaattgt gcactgtcct caaatccttt gccaccctga ccatcatagc
     9241 catcgtatgg tcagggttaa ctattacccc cgaactcagg aacaaccctt caatgtagtt
     9301 tcgacgataa aaggacatgg taagttcaac cacctcattt accgtcaatg ctgtgcgttt
     9361 cacatcattg gtacgcctgt taagacaata ggcgcagtcg tatatgcaat tgtttgtaag
     9421 cataatttta aaaagcgaaa cgcatcggcc atcttcagca aagctatgac atatccccca
     9481 cggagcagca ttacccaaac ctttcccgga atttttgcgg ctactaccac tcgatgcaca
     9541 cgaaacatca tatttggcag caccagcaag aattttcacc ttctccaaag tcttttcact
     9601 taccatctta tgattaaatt ttaatcaatt ttatcgatta aaatttacac ttattttttt
     9661 acaaaagcaa gtttttgaaa aaatttttat tagacagtca acttttttca taaaaaaagg
     9721 tttatatttc tgaaaaaaca agctgttaac aagttcaaca atatttttga aaactttatt
     9781 ggcatttttc tccattaaaa ggttttattg aggaaatttg cataaaacaa ggctaataaa
     9841 tacaaatcag atatgagttt ttttaaaaaa atggaaaatt tagacactcc gcaaagcagc
     9901 aatagtgcgc ttaccggaga agctgaaaaa gtaaaatgtt taatcattgg ctctggccct
     9961 gccggatata ctgcagctat ttatgctgct cgtgctaacc tttcccctgt ggtatatgaa
    10021 ggcatccagc ctggcggaca gcttactact actaccgaaa ttgagaattt ccctggctat
    10081 ccctcaggag taacaggcac cgagatgatg gaagacctaa aaaaacaagc acagcgcttt
    10141 ggcacagaag ttcgctttgg cattgctacg tcagtggact tttcaaaaag gcctttcaaa
    10201 gtagttattg atgaaaataa acttattgaa gcccaggtgg taattattgc tactggcgca
    10261 acagctaaat acctgggcct tgaaagcgaa gaaaaattta aaggttcagg agtatctgcc
    10321 tgtgctacct gcgatggttt cttttatcgc aaacaagatg tagccgtagt tggcggtggc
    10381 gacacagctt gcgaagaagc tacttacctg gccaacttat gcaacaaggt gtatatgatt
    10441 gtcagaaaac ctttcctccg tgcttcaaaa gctatgcaag aacgtgtttt caatacacct
    10501 aacattactg tacttttcga acaccaaact aaagaactta ttgggaaaga tgctgttgaa
    10561 ggtgctttac ttaccaatgc caaaggtgaa ttggtaaaaa ttgacataac aggcttcttc
    10621 ttagctattg gccaccatcc caattctgat attttcaaac catacgtaaa tactgatgag
    10681 gcaggttata ttatcactaa accaggcact tcacagacca atatccctgg agtatttgcc
    10741 tgtggcgatg ttcaggataa aaactaccgt caggctatta ctgctgccgg ctcgggctgt
    10801 atggccgcgt tagatgccga gcggttcttg ggcgaacacg gtttggctta attaattcat
    10861 aaatgctaag acactaaggc gctaataatt aaattttcgc gtcttagcgt cttagcgttt
    10921 aaaatttaca aattatcctt tatgagtaaa ctggccatca tcggtgctgg aaatgttgca
    10981 aaccatttat caaaagcttt tcaaaaggcc gaacatgata ttgtacaggt atatagcagg
    11041 actcagggaa aagctcaagc actttcttct atattaaact gtagttgcac cattgacatc
    11101 tctgctttaa ctgatgatgc tgatatttat ctattttgca tttcagattc cgctattgaa
    11161 gaagttttag aaaaaaaaga ttggcacaac aagcttcttt tgcacactgc cggttctctc
    11221 cctctggaaa tatttaaaaa cagaagttct gactatggtg tactatatcc attacagaca
    11281 tttactacca actttacatt agatataact aaagttcctt tcttcattga agggaataac
    11341 tatacaactt taaaaaccat taaagaatta gcttccagca tttcacctca gatttttgag
    11401 gccgatagcc aaaaacggtt acaactgcat ctggcagccg tatttgccaa caacttcacc
    11461 aaccacatga tggctattgc cagccaattg ctggaagaaa acaatattcc ggctgaaaat
    11521 atcaaccctt tgctggaaga aacatttaag aaagcgcaga caattggagc tgataaagcc
    11581 caaactggcc cggctatccg caacaacaaa gctgttatgg agttacacaa tcatttgttg
    11641 gcaaaacacc ccgaatggca aaaaatatat acttttgtct ccgagagtat caaaaaaaat
    11701 accattcatg gctaattaca gagaaaaact aacaaaaatc aaagcctttg tctttgatgt
    11761 ggacggggta ttgggatcag acaaagtgct gcttttcccc gatggagata tgatgcggag
    11821 catgaacatc aaagatggct atgccatgaa ctatgccata cgtcagggct acaaaatagc
    11881 cattattact ggtggtacat caaaatctgt caagcatagg tttacccgct tgggcattga
    11941 cgatgtatat ctcgaatcga gcaataaaat caaagattat gaacatttcc tcgaaaaaca
    12001 tggcctaaaa aacgaggaaa tattatacat gggcgatgat atgcccgatt ataaggttat
    12061 gaaaatggtt ggtgtagcta catgcccggt tactgctgct aacgaaatta aagaaatatc
    12121 gcactatata tccgacaaag ccggaggcga aggtgctgtg cgcgatgtaa tagaacaagt
    12181 attgaaagtg cagaataaat ggatttctgc cgagtctttt atttggtaac acttaagaaa
    12241 tactgtaacc agatgaagca ttttatcaaa cttattcgtt ataaaaacct tgccatcatt
    12301 gccggagtta tgtatatctt gcgttatggg ttaatactac ctctgctaac ctccaatgga
    12361 ctggcaaacc aactaacaca tattcaattc cttttattga caatagctac agtgtttatt
    12421 gctgccggag ggtatattat caatgatgtt tatgatgttg atattgacaa aataaacaaa
    12481 cccgaaaaac tcatcatagg gaaatatatc agactgcgca cagccgagaa tatatatgtt
    12541 ggcttaactt taattgccat tatttgcggg gcttacgttt catacactgt caacctgcgt
    12601 tcgctgagcc tgttattccc tattgttgca ggactactct acttttactc taccagttat
    12661 aaaagcactg tactacttgg caatgttgct atagcagcaa ttacagcact tgtaccattc
    12721 atggttatca tatttgaact accgctattg actatcaagt atgcctcttt cactggtagt
    12781 tctttcaatt tcaattttat cattgcctgg attgcctttt attcagcatt tgcatttatc
    12841 tcaacactat tacgtgagat tctaaaggat atggaagact tcgaaggaga tgaagcttat
    12901 ggaaaaaaga ctatccctat aacttggggc attaaaatat ccaaatacat taccttatca
    12961 ttaagcttac tgcttctatg cctcgaaata tttatactta tcaaatatct gcatgacctt
    13021 ttttcagtaa tatatattat tccgtttgtt attaccccta caatatttgt tatggttaag
    13081 attttatcat caaaaaccaa agaacaatac catttagcca gcaaatggtg taaaataacc
    13141 atggcaaccg gtattctata tatcattttt gtgagggtgc ttattcttgg attatattaa
    13201 atttcggata accatgttat gtaatttttc taaaagattt tagcaaatag cagtatttcc
    13261 tcctgcctaa gacgggaagg ggttgaggtg gtttataaat tgacataaca caaatttaaa
    13321 gaaaagtgaa atattacatc atcgcaggcg aggcttcagg agacctacac ggttcgaacc
    13381 tgttgaaagc attaaaagaa gctgaccatt cagctgaatt caggattgtt ggcggccacc
    13441 tgatggaaga acaaggcggc actcttttga aacactataa agacatggct tttatgggcc
    13501 ttgatgttgt gttgcacttg cgcaccatcc ttcggttgat gaaatactgc caacaagatg
    13561 tacttgcctg gaaacccgac gtactgattc tcattgatta tccgggcttt aacctgcgaa
    13621 tagccaaatt tgcccattcc aatggcatcc ggacatatta ctacatagcc cccaaagtct
    13681 gggcatggaa agaaaacagg gtgaagcaaa tccgtaagtt tgtcgataaa cttttcatca
    13741 tattcccttt cgaaaaagaa tattttgccc aacataacat tcaaagcgaa tattttggca
    13801 accctctgaa tgacaacatt catgctttca aaaattcaga agatggtttt aacattattg
    13861 ccagccagta tggattagaa aagggcaaat accttgcatt agtagcagga agccgaagag
    13921 gagaagtaaa acacttatta cctcctatga ttaaagctct ggaaggtctt aaccaataca
    13981 aactggtgat tacggcagcc ccgtctatcc ctctggaatt ttaccaaaaa atagtgggca
    14041 ataaacccat tgccattatc tacaaccaaa catatacgct tatttcaaac gcagcagctg
    14101 ccgctgttac atcaggaact gcaactttag aaacagcatt actcaaaacc cctcaggttg
    14161 ttgtattcaa aacaaactgg cccacctact gcgtaggtaa aatgctggtt aagattaagt
    14221 tcttctcgct ggtcaatatt attcaaggca aagaagtagt aaaagaactg cttcaggtta
    14281 atttggaaag aaatatcaga aatgaaatat cgctcattct gaatgacaac aactacaaac
    14341 agaatatgct caacaactac gaagaaatag cccaaaaatt aggcgaacca ggagtagcca
    14401 aacgtgtggc taacagaatt attgaacttt taaaatagtt atgattaaaa aggttctatt
    14461 cgttttgata attatgctgc cttttgtaaa aagtccagct caaaccctca gaatagggct
    14521 tttcagcaac attaccacaa aagcaacagt cttttctgcg gttcagggat cttatcttct
    14581 ttcaggcgat aacagggctg ttttgacatt aaaagaaaaa caaaacatct tattagttaa
    14641 aaaaggtgat tctgtaacat gtatatgcgc agataagcaa aaatacacct ttaaaaaggt
    14701 taaactatca catatatcgc taaatgcttt ttttagcctt cgtcctatcg acccttcaaa
    14761 tgaactgcac tattacgatg acgacctcga aatatatgct gacttaggca aacttcaaac
    14821 aatcaatatt gttgatatag acaactacct ggctggcgtt gttgaagctg aagccggcaa
    14881 taacgccttg ccagaatttt ataaagccca ggttacactt tgccgaacat acacgctaag
    14941 ccacctcgac agacacaacg aagaaggctt ttacctttgt gacggtgtgc attgccaagc
    15001 ctatcacgga aaatgctctg gcaaaaatgc cattgttgat gcagccagaa ctacccataa
    15061 caaagttatc caagacaatg actctgtgct cattacagca gcctttcatg ctaactgcgg
    15121 cggcgaaact gaaagcgccc aaaatgtatg gcttataaaa agaaattacc tggccccgat
    15181 aaaagatccc tattgccagg accgcaacaa tagccgttgg gaacgtaaaa tacctatcga
    15241 ccagtggatt aaatatctta tcagcaacgg gtttactatt aacgaccaga ttagtctgtc
    15301 tttgcttaac aatacccagt ttagccgaaa acagtattac aaaatcggta cagattcttt
    15361 aacttaccga aaaatccgaa acgactttca actaaaatca gccttctttt ctgttgaagt
    15421 aaaaaaccct aacattgaaa tccacggaag aggctacgga catggggtgg gtatgtgcca
    15481 ggaaggtgca atgcaaatgg ctgttttggg ttatacttat gagcaaatca taaattttta
    15541 ttacaaaaac gtaaaaatta ttgatttttc aaatatcaac gcaaataaga accctactat
    15601 caaggtttta tatactaatc cttaacatca atcaagatac ctgccagcac ttcagtatgg
    15661 cattaatcaa atcttcctgc tgcataggct ttgtaagata accatcgcac ccggcagcca
    15721 tacacttccc tttgtcttcc gaaaaagcat gagcagtctg ggcaataaca ggcatttggg
    15781 ggttatattg cttgataagg cgcgtggctt caaaaccatc catttcgggc aaacgcatat
    15841 ccataagcac aaggtcaata ctttcgtctt tcttacacgc ttttaccgcc tcaatgcctg
    15901 tcaccacatg ggtaatctta acagggaaat cttctaacaa agcttttata agcattgcac
    15961 tggtaatatc atcttcaacc agcaaaatat gcttatcttc ccatttaaac gatgccccat
    16021 tatcagcctt gataataacc tggctcttaa cattgtatgg tatgataaat gaaaatttac
    16081 tccctccccc tttcttcgac tcaacccaaa ttgtccctcc catggctttt acataacctt
    16141 tagctatggc aagccctaaa ccattacccc catataaacg cttagaagac atttctacct
    16201 gcttgaaagc ctcaaaaata agctcttttt cttcttctgc tataccaatc ccggtatctg
    16261 aaacaaaaaa ctcaagataa tcttcgcgct tgctacatcc aaacctaatt tggccattat
    16321 cggtaaattt cacagcattg ctcaacagat tttgcataat aagccttaac tttggctcgt
    16381 ccgtcacaac cggctccttt aacaacgcat caagaggctc aagtacaagc tctatttgag
    16441 tttttccacg tattaacata taatttttaa aatactcaaa taactcgccc atcagcttcg
    16501 gaagctctac aatattagaa aacaactcta tttctccaat ttctattttt gaaacatcta
    16561 ccaaatcaga tactatcttt accaattgct ttgaactatt aataataatc tgaatatatt
    16621 gggccctttt ttcgtcatct ctacaatttt taagcaattc ggcaaaccct ataataccat
    16681 tggccggtgt cctaagctcg tgagaaatgt tacataaaaa cactgatttg aggcgctctg
    16741 ccacttcaga tttttctttg gcagcaatta gctcctcttc ataaatcctt tgagatgtaa
    16801 tatcaaatgc cacaaacgaa aacgaattgc tgcccgataa ctccaaatta gcagctgtta
    16861 tcatcacatc aagccgcacc ccgtttttca tcctccaatg agtattgacc gtagaggtat
    16921 tttgctcaac aagataagtg gacaggattt tatttacgtt caaatattcg tcctcatctg
    16981 caaataaaaa tgaaaaattc ctcccgctaa tctcttccat gttgtatccc gtcatcaatg
    17041 aggcatgttc attagcaaac aaaatgatat tatctgaaac taacccaata aaagccggga
    17101 cactgtttaa aatgttatca agcaacttct gcttttcctg caattgtgta tataacttat
    17161 ttattttctt taaactaatt tcaagctgac tgtttacttt ctcaaaactt tcactctgtt
    17221 cggcaagtat ctgttcattt tgtcttattt gttcctcgta acttttacga atgtcaatgt
    17281 ctgcaacagt gccaagccac tttctgctat ttgcatcttc aaacccttcg tccagcatag
    17341 catttatttg tacccactga tatataccct tttgattgcg cagcctacat tccagaataa
    17401 agtttgaagc atccgggcct gtatattcaa tagccttaat tattcgctct ttgtcttctt
    17461 catgcaacaa tttatgcagg gtggctatat cttgctcaaa ctcaaatgcc tcatatccaa
    17521 gaatttctaa aaacctgtca ttaaaactaa acttctttgt tttaaattca tactcccaaa
    17581 taccctcatt gatatgggta aaaatttttt tatcaaccaa ctcttgttca acaatctttg
    17641 aggcaagaat ttcattatat gacataagct gcttaagctt ttctttcata ccggtcaatt
    17701 caaacatcaa gttgtcattc atgcctgatg aattgaaaaa accgttttta tccttcaaaa
    17761 cacgttgatc gtaccactga ttggccggaa taaaaagaaa attatacctg ttttcatcca
    17821 aaggcatccc cacaacaaaa gcctcaatat ttttcccatt caaagaaaag gttgaacgca
    17881 ccctgatgac ttcttttctt ttcatttctc ctatcaaata atctgcttct ccttcactca
    17941 gcatctcata cacagaagct ggtacttttt cgtttgtatc aagcccccat aagccggaaa
    18001 atttagcatc tatataaagc agctttccgt ctgcagaaag atcaaacaga ccaatttgaa
    18061 gtacatctat aaatttgggg ttcacagggt tattcatgat ttcctttttt gttttttcga
    18121 ccgggtcgaa atcctataca agttagctat tttttatttt ttgtccaaat ttcttttttc
    18181 aacttcttct ttatttagaa tcattttaac tttattgtga attttgtaac agtgttattt
    18241 ttataacata aataaatatc tttgcaaagt caactaacac tcaacgaaca acttttttac
    18301 gctgaaaaat aaacgctttt ctattaaact actgaatttt aaacttgatt taaaataaaa
    18361 catatatgaa aacaagcatc ttggtagaaa aactgggagc taatgtggtt tgcggaaaag
    18421 aacatttaga aacaaaagaa attgagtatg cttttgcttc tgatttgatg agtgatgtac
    18481 taacaatcaa tgcaaaaaac attctgctca ttacaggcat ggccaatata caaaccatcc
    18541 gtactgccga aatggccgac attcacacca ttgtgttagt ccgcaacaaa aaagccaccc
    18601 ccgaaatgat tgaacttgct atgcaaaacg atatgattat tgcagaaagc tcaaactcaa
    18661 tgttcaaaac ttcaggtatt ttgtacaaca tgggattaaa accggtatat tgaccgctat
    18721 gcaactaaaa tttcacatcg aaggtggtaa ttttaccaaa gctggctttg cttccagcga
    18781 agtaaaaaaa tatttacgca aaatcaatat ttctaacgaa accattaagc gtattgtcat
    18841 tgccatgtac gaagccgaag taaatgtagt tgcacatgct tatgaaggtg aaatcctggt
    18901 caacattgac tcaaaaaaag ttgatatttc tgttattgac aaaggacctg gcattgaaga
    18961 tattgaattg gctatgaaag aagggttttc tactgccagt caaaaagtcc gcgaaatggg
    19021 atttggtgct ggcatggggc tttcaaacat caaacgcaat gcaaacgaat ttatcataaa
    19081 aagccaaccc ggagaaggta catctctgaa tattattgta tatttaaaca atgtaaacta
    19141 atatggaaca aacgcatttt caccatgcat taaaagttat gccggagctt tgcacggcat
    19201 gctcgcattg tatgcgttcg tgccctaccg aagcaatcag gattagaaat ggcaaagcca
    19261 atattaccgg caacaaatgc atcgattgtg gcgaatgctt cagagtttgc ccttctaatg
    19321 ccatttatgt tgaacaagac gattttaatg ctatatttaa ctacaaatac agggtggcaa
    19381 tcgtgcctgt tgtccttaca gcacaacttt cgttactcta ttcaatggac gaaatattct
    19441 ctgcctttac caatattgga tttacccata tttatgaagt tgaaaacagc gcccacattc
    19501 ttcgccagtt tcaacaagaa tatattgccc aaaacaaaga attcaagccg ctaatctcgt
    19561 gtttttgccc tgctgttgtg cgaatgatac aggttaaatt cccttcactg acaccacacc
    19621 tgattacgct caaacaacca cttgactatg cagccatgta ttgccgaaaa agacttgaag
    19681 atgctggtat ttctgaaaaa gaagcaggca tattttatat taccccatgc gccgctaaaa
    19741 tagctgcagt taaaagccct gtaggagaag aaaaatcagc cgtaactgga gttatcaaca
    19801 tggatttgtt atacaatcgc attcgcagaa atataacaaa caaccccggt gccaaacaaa
    19861 acgacattat tccatttgaa aggccttcaa aaataggata cacctggccc ttaactaagg
    19921 gtgaagctaa aaacattaat ggcaggactg tggctattga tgggctaaaa aatgcattag
    19981 attttctgga aaaaattgaa aacgaagaaa cttcaggctt tgactttatt gaaattcgtg
    20041 cttgcgacga aagctgtgcc ggcggcatcc tctcttccac caatcgtttt atggtagtag
    20101 aagaactgga aaaagaagcg aatgctgcaa aaaacgaacc taaaaatatc gaccaatata
    20161 taggtttctt gcatgctaat aaagacattc aagcagttga accccgaagc atcatgcaat
    20221 tggacaatga tacaaccaaa gcacttgaaa aagtccagaa gatacaaaaa ctaatgtgct
    20281 ttttaccagg ttttgactgt ggcgtatgcg gggccccatc atgcaatgct ttagcagaag
    20341 atattgtaca aggtcatgcc catatttcgc actgcctttt catgcaacgc gtaatggaac
    20401 aaaaacataa actcaatccg gaccatgctt atcatattat tgagcaaatc tggggtgaga
    20461 aaagattaga aaaaaactgt aataagaaag gagctaaaca atgaatgtca aagatattgc
    20521 ctctttgata aatgccaaag tagtaagtgg ccaaaacggt ttagataaag aagtaacagg
    20581 agcttacaca tctgaccttt taagtaatgt aatggctaat attgaagaaa accaaatttg
    20641 gattactctc caaactcaca aaaacatcat agccatagca tcgctgaaag atattgcagc
    20701 catcatcctg gtacaagatt tattgccctc tgaagagtgc gccgaacaaa gtaacattga
    20761 aggaattcct attctttcaa cttcgctcaa tgcttaccaa attagcggaa agctttttga
    20821 aattctcaag aataatgctt aactttcata aagcaaacta ataaatataa caaactgaca
    20881 accaaccaat aaagctccct caagtgaacc gatacaaagc agacctgcat attcacactg
    20941 ttctctctcc atgtgccaac attgagatga gtccggtgaa tattgtcaaa agggctttaa
    21001 tgaaagggtt aaacatcatc ggtattactg accataactc gacacttcaa tgtgccactg
    21061 tgaaagaatt agctgcccct catggcatta tggttctttg cggagccgag gttacctcaa
    21121 aagaagaaat tcactgcctt gcattctttg aagacgatga atcccttgca gaatttcaga
    21181 aatatctgat gcttcaccag cttaagcagc caaatacaaa caattcattt ggataccagg
    21241 tagttgttga caaagacaat aatattacgg ccgaaattga caactacctg ggtatggcta
    21301 tggatgtagg ttatttgcaa atacaagaaa tagtacacca gctcaacggg ctttttatcc
    21361 ctgcccatat cgacagggtg cgtttcagcc ttatgtcaca gctcggattt gtccctaacg
    21421 acattaatgc tgatgcgctt gaaatcttta accggacctc catttctcgt tttatgaaag
    21481 agaatggcta ccttacccat tatactttta tcaggaactc tgacgcccat cacctcgatg
    21541 atataggcca atattacagt tggtttatca tgaacgaact ctctttttct gaaataagga
    21601 tggccctgaa atacaaaaac ggaagacttg taactattga ttgacagatg gaagaaatag
    21661 ctttacatat tctggacatt gtacaaaact ctattgtagc ttgtgctaac ctgatttcaa
    21721 tagaggttga agaagactat gccaacgaca ggctcattat tattatcgtt gacaatggca
    21781 agggcattga aaagcacctg ttagataaag ttgcagatcc attttacacc aaaaggacaa
    21841 cccgaaaagt tggacttggc ttatctcttt tgaaacaaag tgcagaaatg gccggcggaa
    21901 gtttttcaat agaatctgag ataaataaag gcaccactgt aaaagctata taccaacatt
    21961 ctcatattga ccgccaaccg cttggtgaca taccacaaac cataagtata ctggttgctt
    22021 caaaccccga cattaatttt acttataaac ataaaataaa caacaactca tattactttg
    22081 ataccaggca aataaaggaa gttttggaag aagtacccat tacttcgcct gaagtacgag
    22141 tatttataca tcaaatgcta aagcaaaata ttgacatgct taacagacaa ttcaaagaaa
    22201 tataaaaaat tcaatatttt caatattatg gaaaaagtta aaacgttaga cgacttaaaa
    22261 aagctgaaag aacagcttaa atcaacaatt gagttgcgtg aaaagagtaa taaccccgag
    22321 agccacgtac aggtaaaagt agctatggct acatgcggta tagcatccgg cgcccgcgaa
    22381 atcatgtcct tcttcatgga cgagctccgc aaacgtaaca tcgatgctat tgtttctcaa
    22441 acaggctgta tgggatattg ctatgccgag cctaccatag aacttactat ccccgggcac
    22501 gaacctgttg tctatggtaa tgtaaccaaa gctaaagcag atgaaatcat tgagaaacat
    22561 atcaaaaacg gcatcctggt agatggtatc atcccggtta cctaccaaaa tatcaacaaa
    22621 aaataaaatt ttgacattaa aatacttata acaatgaaac tcccatgttt tggcaaacat
    22681 aaagacaatg aaaacaaaat cagctgcatg gggttccatc attccttata aaaataattt
    22741 tgttatgatg aaatataaaa tgcatgtttt ggtttgtggc ggtactggct gcaaatcgtc
    22801 cgacagcgac atgattgccc acaatctgcg cacggcaatt gaccaaaaag gacttacaga
    22861 agaagtacaa gtagtaatga gcgggtgctt tggcttttgc gaaaaaggcc ctatcgtcaa
    22921 aattttgccc gataacactt tttatgtaca tgtaacaccc agtgatacta ctgagattgt
    22981 agaagaacat cttgtaaaag gcagacgtgt tgagcgctta ttgtacaaag accctcagag
    23041 ggatgaaata attagcgact caaagcacat gggtttctac aaaaagcaag tacgtatagc
    23101 tctgcgcaac tgcggtttta ttgatcccga aagtattaac gaagctattg cccgtgatgc
    23161 ttatcaggct ttaggcaaag tattgaaaga aatgacccct cagcaagttg ctgaagaaat
    23221 taaaaaatca ggccttcgtg gacgaggtgg agcagggttc cctacaggtc tcaaatggga
    23281 aatagcctct aaatctcccg gccctgagaa atatgtagtt tgtaacgcag acgaaggcga
    23341 ccccggtgct tttatggacc gctctatact cgaaggcgac ccacactcag tattagaagc
    23401 tatggctatt tgtggatact gtattggagc atcaaatggg cttatttaca tcagggccga
    23461 atacccatta gccattgaac gcctgaaaat agccatcaat caggctcgag aatatggatt
    23521 acttggcaaa aacatatttg gtacagactt tagttttgac atcgaactaa aatacggtgc
    23581 aggagcattt gtttgtggtg aagaaactgc tttaatccac tctatggaag ggcaacgtgg
    23641 cgaacctact ttcaaaccgc cattcccggc tgttgctgga tacaaaatga agccaaccaa
    23701 cgtaaataac gttgaaactt atgcaaatat tcctgtaatt ataatgaatg gagccgaatg
    23761 gtttgccagc tacggtacag aaaaatcaaa aggaacaaaa gtttttgccc ttgccggaaa
    23821 aatcaacaac gttgggctta ttgaagtacc tatgggtaca aaattaaaag atgttattta
    23881 cgacattggg ggcggcatta aaggaaataa aaaattcaaa gctgtccaaa ttggaggacc
    23941 ttcaggaggc ttcttaaccg aaaaacacct tgaaactgaa attgactacg aaagccttac
    24001 agctgccggt tcaatgatgg gctccggagg tatgattgtt atggacgatt ctgactgtat
    24061 ggtggctatg gctaaattct acctcgactt cactgttgaa gaatcttgtg gcaaatgttc
    24121 accctgtcgt atcggaaaca agcgcctaaa tgaaatcctg gaaaaaatag cccagggcaa
    24181 aggcactatt gaagacttag aactgttacg caaccttgga aacgttatta aagacacctc
    24241 attgtgtatg cttggtggtt cttctccaaa cccggtacta tcaacacttg accatttcta
    24301 cgacgagtac cttgcacacg tgaaagacca caaatgccca gcaggccaat gtaaagctct
    24361 tatgcaatac attattttac ctgaaaactg cgtaggatgt acagcttgtg cccgtaattg
    24421 ccctgtcaat gcaattaccg gtgagcgcaa aaagcctcat gtcatagatc aggccatttg
    24481 tatcaaatgt ggcgcttgtg ttgaaaaatg taagtttaat gctgttgttg tccaataacc
    24541 gaaaatattg aaagtcatga taaaaattac aatagaccat aaacccattg aagtcgaaaa
    24601 gggaaccacc gtgctaaaag ctgcccagca agccggaata catatcccta ccctttgcta
    24661 tatgcacctt gaagacctaa acatcgaaca taaacccgga ggttgccgta tttgcgttgt
    24721 tgaagttaaa ggccgtaaaa accttgctcc tgcctgtgct acagaatgtt tagatggcat
    24781 ggaaatcaac actcacacta ttcgggtttt aaatgcgcgc aaaaccgtta tggaacttat
    24841 tttgagcgac caccctaccg attgccttgt ttgtgctaaa tctggcaact gcgaccttca
    24901 gtcaatggct catcagctcg gtatccgcaa aatacatttt gaaggcgaac aatcaaccta
    24961 ccgcgaagat acatcgccat ctatcatccg cgatatggac aaatgtatta tgtgccgccg
    25021 ctgcgaaact atgtgcaaca acttccagac tgtaggcgca ttatctgctg taaacagagg
    25081 ctttatggcc gttgttgccc ctgcatttga acaaaacctc gatcattcag tttgtaccta
    25141 ttgcggacaa tgcgtggctg tttgcccaac aggcgctctt actgaggttg accataccaa
    25201 tagcgtaata cgtgcattag cagaccctac aaaaacagtt attgtacaaa cagccccggc
    25261 tgtacgtgcc gcattaggcg aagaatttgg ccttgaagcc ggaacattgg ttaccggcaa
    25321 attagtatct gcgcttcgtc aacttggctt cgactatgta tttgataccg actgggctgc
    25381 agaccttacc attatggaag aaggaacaga gctgctcgac cgtcttgctc gtcatctaaa
    25441 tggtgataaa acagtggctc taccaatatt aacctcatgc tgccctgcat gggtaaaatt
    25501 cttcgaacat caattccctg atatgattga tattccttca tctgcacgtt ctccacaaca
    25561 aatgtttgga gctattgcca aaacatattt tgcagataag attaaagttg accgtaaaaa
    25621 cctcgtggtt gtttctatca tgccatgtct tgccaaaaaa tacgaatgct cacgtgaaga
    25681 acttggccat gacggaaatc cagacgttga cttctctatt tcgacaagag aattggccaa
    25741 ccttattaaa caatcaaaca ttgactttac ggcacttcct gatcaggatt tcgacaaccc
    25801 acttggtgaa tctaccggtg ctgctgttat ttttggcact accggaggtg ttattgaagc
    25861 ggctgttcgt actgcttacg aattgcacac caaaaagaaa ttgcccaaaa ttgacttcca
    25921 cgaattacgc ggtttagatg gcataagaca agcaaccata gactttgacg gattaccaat
    25981 caacattggt atagcccatg gacttggaaa tgcacgtaag ctccttgaag atatcaaagc
    26041 tggcaaatct aaattccatg ctatagaaat catggcttgt cccggaggat gtattggcgg
    26101 aggtggacaa ccattgcatc acggcaactc aaaggttatc aaagctcgtc acgaagccat
    26161 ttacaaagaa gacgcactta aacctataag aaaatcgcac gaaaatccat atatcaccaa
    26221 aatatatgaa gaattccttg gcaaaccgat gagcgaaaaa tctcatcacc tgctacatac
    26281 catatatttt gaccgtagca aaaaaaatgt gatcatcagc gatgaacatc aagccgatta
    26341 attctttggt atgttttaca acttaaatta ttgattatgt caagtataaa aatacagctt
    26401 aagcaatcgc aaatagataa gattgccgaa atatgcaaac aattcaacaa tgacgaaggt
    26461 gagttaatca acgtgcttca caaaactcaa cacacctttg gctatttacc tgctgaagta
    26521 caggaagtta ttgcccgtga attaaaaata ccagtggcaa aagtttacgg cgtggttacc
    26581 ttttattcat tcttttctat gctacccaaa ggcaagaacc caatttctat ttgcatgggt
    26641 acagcctgct acgtacgtgg tgccgaaaaa gtgttggatg aattaaaaaa agaacttaac
    26701 atcaaagttg gcgaaaccac tccggatggc aagttctcac ttagctgctt acgttgtgta
    26761 ggcgcttgcg gccttgcccc tgtgctgatg attggcgaaa aaacttatgg ccgcgtatct
    26821 cctgaaggga ttaaagacat tctgaaagaa tacgaaaacc agtaactgta aatctctgaa
    26881 aatctttaag ataaacaatg ttgacattta ttctgtgtta acattgtttt cttttgcttc
    26941 taagcaaaaa aaattacttt tgcaattcat tgtaaaacac ataaaaataa atataaaatg
    27001 agaaaaaaaa tcgttgcagg taactggaaa atgaatacta cactgcaaga aggcgtaaaa
    27061 cttgcccagg aagttgaagc tttggcagcc agcgtatcaa atgatgctct tattattgtt
    27121 tgcacacctt tcacccactt aaccgaagtt aaaaaatcta tcaagaaagt aaagcttggt
    27181 gctgaaaact gtgcagctga agtatcaggt gcttatacag gcgaagtatc agcttcaatg
    27241 gttgcttcta caggtgccga atatgttatc attggccact ccgaaagaag agcatactac
    27301 cacgagacag acgaaattct tgccaagaaa gtaaaacttg ctttggcaaa caaccttact
    27361 cctatattct gtattggtga gcttttgagc gaaagagaag ctggcaaaca ctttgatgtt
    27421 gtaaaaagcc aactcgaaaa aggcttattc gacctttctg ctgaagattt tggaaaatta
    27481 gttattgctt atgaacctgt ttgggctatt ggaacaggta aaacagctac atctgcacaa
    27541 gcacaagaaa tacacaaagt aatccgtgaa gcaatcgctg ctaaatatgg caaaactgtt
    27601 gcagacaata cttctatcct ttatggagga agctgcaacg acaaaaatgc tgctgaatta
    27661 tttgcaaacc ctgacgttga tggtggtttg attggcggag ctgcccttaa agctgaaagc
    27721 tttatcacca tcgctaaatc attctaaaat taacacaaag aaaggatatg gcgcttcatt
    27781 gagcccgctc ctttcttttt cattatcact tataaactat ttaaaattaa tacattatgg
    27841 ccgaactcgc tgctcccaaa gtgatactaa agaaaaaaca aatccctgta attattgatt
    27901 tttgcttaga cgaatcaatc gaatttagtg taaggcaaca agctttccca gaaacagact
    27961 gggaagtaga actcaaattc aaagatttta aaacagcttt attgacggga atgtttttgc
    28021 gcgaaaacaa atttgaaata gaaggcattg acgctcaaag atataagaaa tcagctactc
    28081 ctgcaaagaa aacagaagaa aaaacagatg ctccaaaagc agacccccaa aaagcagaga
    28141 aaaacaaacc tgaaacagac gaatttcaag ctccttcatt tttgtaaaaa cacatttcga
    28201 tatacaagcc tgtactgtta acatacacgg tacaggcttt ttcattttac acaaaatcaa
    28261 cattttataa ataaaattgt ttatatttct tgttttttaa actaaattag cttgttaaac
    28321 tatcatataa aataataaat ggccatttgc agcataaata aattcacaaa accattgata
    28381 ataaaggcct tttgcttctt attagttact tatcctgctt tttcaacaag cttctctcaa
    28441 aatttaaaag aattatttct tgatgccgaa tacagcctcg aaattatgga atttgaacca
    28501 gccttaaaaa actaccttat tcttcttaaa aaagacccag aaaactgtaa tctaaactac
    28561 cgggcaggaa attgttgcct tcaaataact gaaagaaaaa aagaagcatt aacttactta
    28621 gaaaaagcca ttaaacaaat ctccgtcaat tacaaagctg gcagttttaa cgaaaaaaca
    28681 gcccctcctg acgcctttat gctttatggc attgcgttgc taaataataa caaaataaac
    28741 gaggctcgcg aagcctttaa aaaatacaaa gactaccttc aacctaaaga agtagccgag
    28801 ctcgatgctg taaaccaaca acttaaatac tgcgacaatg ctgaaatttt cagccaaacc
    28861 agtttaaata tttctgtaac tccattaccc atatctttta accaagctaa accaaaatta
    28921 agaatggtta ccaatgccga aaaatcacag atggtttttt tagccgacaa gggtgggaaa
    28981 ccaggaatat tttacacata tcttactaaa gggcaatggt caacaccaac agaaatcacc
    29041 aaagacttag aaatcgaccc caatatagac ttattcaata tctgttctat gtcagctgac
    29101 ggacgatggc tataccttga aattacagac gagtttgatt ctcgtattta tgttgcaaaa
    29161 ttgataggca caaaatggag cgaatgtacc ccgcttaacc gcaaaataaa ttcttccaat
    29221 gatgaaactc atgctgccga atcgcctgat ggaaaagagc tgtacattac ttctaatcgt
    29281 aaaggcacca ttggcaacct tgacatttat gttagccgcc ttaaaaccaa tggagaatgg
    29341 gataccccaa ctaacttagg aaataccata aacacctatt ttaacgaaga aacaccattt
    29401 gtaagcaatg acaaccagca cctgttcttc agttctcaag gacattccag tatgggcgga
    29461 tatgatattt ttgtctcaga tcgcttacca aatggcgaat ggggcgaacc tcgaaacatt
    29521 ggatacccca ttaataccac ctctgacgat ttgttctatt ttccattaga ctctaacaaa
    29581 gcattatatc agcttgacca ctctgcccct tttgccatct cccaagtgga gatattacct
    29641 aaagtcaaca acattatcaa tacagacacc ctcaatttag caaatgctga tacagtaaaa
    29701 acagatacag taaaacaggt tactcatgta atcgaaacat ttgatactac aagtaaacca
    29761 attgttgaaa aagatactac tccaatcgct cctgttgttg cggaacctgt catttctcaa
    29821 ctaaaaggaa aaataagctt ttctgacgat tcaaaaagtt ttgacataat aaatgccttt
    29881 attatggata aaaacaacaa caaaaaaagc ctatcaatca gaagtgacgg cacttttgac
    29941 caaactattt ctccaggtca atacaaaata tcttttgaag ccaatgaata tcaaaaagta
    30001 attaaaaaca tcttgattac taaaggacaa cctgccgttt tagatatcat gatgcgacct
    30061 gaggtaatta aagaacaata tgtatcaatc cgcagcattt tgtttaacta taattctgta
    30121 ttacttgata atgaatctaa aacaatgctt gaaaggctca tcagaattat gcgcgattac
    30181 ccttctttaa caatagaaat tattggacat gccgatagca aaggaaaccc tgaatataac
    30241 cgaaagattt cgctcgacag ggccagagcc gttgccgatt attttgtcaa ccagggcata
    30301 gatgaaaacc gcttcacttt atttggtttt ggagatgctt tagccattgc cattgacgta
    30361 aaccccgacg gttccgacaa tccggctggc agaaggttca accgaagagt agagctaaga
    30421 ttagcaaatt acagcaattt aaagattgta aatacagaat tggaaatccc tgaaaactta
    30481 aggattaaag aaacctttga atattatatc tgcctcaaag aaagcccaac aatggtttca
    30541 aaagatgaat atatttcaaa agctgacaca tcaatgatta aaattaataa tattggccaa
    30601 gggtacattg ttactctaaa ccctccaatg tcaaaagctg aagcaataaa aaaacttgaa
    30661 gaaatcaaca acacattccc tgaagcttac attatccctg acaactattt tacaaaagtt
    30721 aaaaaagtca gtactgtagg ttcatatcgt tatgcatcat caggcaactt caccattcaa
    30781 cttcttgcca caaaaagaaa aatgaaaact acagcattta agccctatac agatgttaga
    30841 gagtacaaag gcatggatgg cttgctccgt tacacttatg gcctattcag ttcatgggac
    30901 aatgcttacc caaccctcct aaaagtaaga aaacaaggat ataacgatgc tttcattgcc
    30961 cctatggcaa aatttacagg aaacctgctg gataaaaagc tgaatggcta tacaattcaa
    31021 ttatggactg gatttacacc taaagacacc agcatattta gaaatgtagg accaataaaa
    31081 gttaacaaaa cttcagacaa cgtttaccgc tattcaacag gtacatttga aaccttcccc
    31141 gaagccaaag ccaggctgaa acagttgcaa aagcttggat actccaatgc ccttatcaaa
    31201 aagcaaagcg acatggaaaa atagcgtttt aatattacat ttgctggcta ataaagacat
    31261 atttggtata tggaacaata ttttcttgac ctggtaaaaa aacgtcaaag tgttagggca
    31321 tacaaaacag atgcagttga acctgaaaaa atagcccgtt gcattgaggc tgcccgctta
    31381 tcaccttcgg catgtaatgc ccaaccttgg aaatttattg ttgttgacga ccagaaaata
    31441 aaaaaccaga ttgccgattt gaccacaact aagatggttc caatgaacca tttcaccaaa
    31501 caagccccgg tacatgtagt tttagtgatg gagaacccaa acttaacttc ttttgtaggt
    31561 tctgtaatta gagataaacc ttttaccttg atagacatag gcattgcagc tgttcagttt
    31621 tgcttgcaag cctcagccga aggccttgga acatgtatga ttggatggtt caatgaaaag
    31681 aaagtaaaga aactgctcaa tatcccaagc tcaaaaagag ctgtattggt tattaccctt
    31741 ggttaccctg aggatgacac aatacgtgaa aaaatacgta aacctgtaga gcagatttat
    31801 agccgcaatt catactaatg tacctgcaat ttcagcagta caggcaagtg gtcgctatat
    31861 ccacctgtat atttcatccc atcataagtc tctaaaggct ttttgccccc atgcctctca
    31921 tcttcctgaa gaataaaagg cagtgcagct atttctgctt tggaaacaca aagtcctgtt
    31981 gttttatcag ctaaaaaacg actcacaatc acctgatcaa acacattcca cttgccttta
    32041 tatttaagtg ttcctattct attaatattt ccaacccatt cagcactaag gtttaccaaa
    32101 tcacctttat ttacagcttc tgctttgcct cttgcccgca atgtattctt aatagacata
    32161 ttgtccgggt catcattaaa gtcccccaca accacaatgc ttgaacggct attttgtttc
    32221 aggatagaat caacctgaac ccgcatgaca gcagctgcaa acattctttt ttgttcactt
    32281 tttagctccc ctcccaacct cgatggccag tggttgataa aaatatggac agtgtcagtt
    32341 tttgccaaca ctcctttagt ataaacaata tcgcgggttc tcaactgtcc ccgtcccttt
    32401 ttgccaataa ctttaataaa ctttgaactc aatggcttaa ataagtcagg ccgatacaat
    32461 aatgcagcat ctatacctcg aggatcgtcc gattctttgt ggattatttt ataattgaac
    32521 ttacttaacc cggtattctt aaccaaatca tccaacaccc tttcattctc tacttcggcc
    32581 aaacccacaa ctacaggcat gtcaacatta accgataaga taaccttact gatattatcc
    32641 aattttttct tgtaccgttc aatagtccag taatgtttag atgttggcaa aaagtcatta
    32701 tcattttttt caggatcatc ttctgtatca aaaagatttt caacattgta aaacataata
    32761 ccaacagaag ctacagcctt cttttggccg aaaatactgt atattcccaa caaacagaaa
    32821 ataaaacaaa acgcaaccct gaccttcata aacctataaa acgattattc aatttccctt
    32881 tgctaattgc aggaaatatc aagtcccttc ttttccctta tttattttca gcaggagcaa
    32941 catattcata gcacccggca tctggcattt catctacaag acggctattt tcaattaaat
    33001 caaccggata caaattggca atatcaatac tgccaatttc gcgggctatt gatgtcctgg
    33061 ttagctgaaa atcgtatttg taattaatta catcaacctt aacaaatcca ggctctgctt
    33121 tatctgacac tttagtggta agaaactttg tttcgtcaga aagattgagg ccgttcttcg
    33181 gatatttaat taaacaatgg tcaaaaaggt aattggcggc cttcttatct gaaaactcaa
    33241 gaacaagaga ttttacattt tcgctaaaaa tcaaacaatt gccaaaatac gccttggtaa
    33301 ggttttcata gtaatatttc ttagcgtata tatttgaaga ggtcgtatcg ctttcaacag
    33361 cataattcat caaaacaaca gcgctacctc cactaatccc aaatgacgga taattggcaa
    33421 atgtgcaatg ataaaactca tattctccac cgggcgagca catgaatgag gcataaccgg
    33481 cattggcaat aagcgaattg ttagctgtaa cctttgcatt gatagcaaac aggcccacta
    33541 tagcatgatt gtatatctta cagttcgaca gctccaactc tgccttttct tctttattca
    33601 gcacccctgc cacaatacca aactgaccat tgcggataat ggcataattg agttttccct
    33661 tactgccatt cataaacctg atttcttccc attgcccggg aatctcgtcg taaggaacag
    33721 gaggtgcata gttcgaaaca tccatcctgt cacctctgaa aactacagga ttttcttttg
    33781 taccattaac aatcattgtc cccttaaccc caaaccaaac tccgcggtgc atatatattt
    33841 ttgtcccggg attgatggta agcgtggcca aactatctac caatagggct ttatatatga
    33901 catagggctt gtcatttgtc caagtctgcg tccctataat actatctttc agtagatgta
    33961 tatcctgccc ccaagcaagc aattttactt tttgcaaatt attatttgta ttaaaaacaa
    34021 gggaatcttc caacactaaa ggcgaatttt gccctgttgg gtcaactttc acctgaacaa
    34081 aaatatacat gctgtcatgg gcctcaatcc taaaattgct ggctgtatct gccaaaccat
    34141 taatatttat tataaaagga gactgggctc ctccggccac ctttacagat tttatttcaa
    34201 ttggctgatt caatggatta tacactttca accttttggt agtacttccc aatgtagaaa
    34261 aaacagtatc aaacataact gtatctgtag aaaattcaag gcgagcatcc gatccggtat
    34321 atactgtatc atcttcacaa ctgaaaacca acaataacaa tgcaacaaca cttatcaact
    34381 gaaataatct tctaaccata ttaaatcatt aaatgaaaat ataacgcatt ggtcggcgaa
    34441 aaattttagc caacaaaact aaaaaagctt tttgcttttt aacggcgaaa atacaggatt
    34501 tttctaattt taacgcaaat aaaaaaatat gtttcaggct ttaaaacacc catttttctt
    34561 actttttgca atacttctca cagggtgtaa cacattcagc cctaatcaca acaacatcat
    34621 acggtacaac gagtctaaag gcataccgac actcgacccg gcttatgccc gcaattaccc
    34681 ggccatatta gctgttgttc aattgtacaa tgggctgcta caactcgacg actctctcaa
    34741 tatcaaacct tgcatagcaa agaagtatgt tatttctgaa aatggaacag tttatacatt
    34801 tactctccgc aatgatgttt tttttcacga cagccctttg tttgcccagg gtaaaggaag
    34861 aaaagtcaca gcctccgact ttatttacag ctttttacgt gtcatgaaca aaacaacagc
    34921 ctcgccaggc tcatgggttt tcaatggtat tgatacaaca aaggccaatc atggatttga
    34981 agctacaaat gacaccacac taaagattta cctgaaagtt cccaatgcca gctttgctgg
    35041 tatcctcacc aattcttatt gttttgttgt cccacatgag gtagtcgatt attataaaaa
    35101 agattttggc acacaccctg tcggaacagg ggctttcaag ctaaaactct ggcaggatgg
    35161 cgaaaaactc gttttactaa aaaacccttc ttatttcgaa acagacaaat ctggaaaaca
    35221 ccttccttac cttgatggta tttctatttc ctttgttgca gacaaacaat cagaattcat
    35281 gatgtttatc aaaggaaata tcgattttat tattggcgta aacgcttcgt ttaaagacga
    35341 acttatcaat atggaaggaa atatcagaaa agaatataaa aaccgctttt acatgctcaa
    35401 atgtccttat ttaaataccg aatacctcgg ctttcaactt gataacaatt taccggcctt
    35461 taaaaactct cccttacaca atctttatat caggcaggcc ataaatatgg ccatagaccg
    35521 aaagaatatg atacaatacc tccgcaacaa cctgggatac cccgctgagt atggtttcac
    35581 gccaaacggc atgcctgact ttgaaaaaat gaaaacaaaa ggattttacc acaaccctga
    35641 ctcagctatg aagctccttg ctaaggccgg ttttcctcag ggtgcaggac taaagccttt
    35701 caaacttttc acctcatcag attatcttga cctctgcgaa tttatccaac acgaactcga
    35761 aaaaataaat ataacatgca acatagaagt tactaatggc cttgcattca gagatatgat
    35821 agccaacaac aaaatggaaa tgttccgtgc ttcatggatt gcagattatc cagaccctga
    35881 aaactactta tcgctctttt attccaaaaa cttctctccc gcaggctcta actacaccca
    35941 tttttcagat gaaagctacg accttcttta cgaaaaggca cttaaaactg taaatttgaa
    36001 cgaaaggtat ttgctatacc gccagatgga cagcctgatc attgccaatg ccgccattgt
    36061 accacttttt tacgatgtag ctgtcaaaat tgcccacaaa aaaatccaag gcctgagcaa
    36121 taaccctctt aacctcatcc agcttaaata cgcttcaaaa caataaaaac ctgaataatg
    36181 tttttagaaa ttaaaacaat ttcttaggct tatattttga gcatttttca tttaaacttt
    36241 ctaaaacgat gtcaattatt gcacaaaaat tttctaagtc aaaaatccac ctcatttttt
    36301 tatcttttta atattcaagc agcaggtaat acttttttgg ttaaaaataa agcccacatt
    36361 ttacaagggt tggaaataat aaacaaatta tcaaacatcg taatttattc atattaaaaa
    36421 acttacaaga aaaattctca cagcattgat tttcaatcat ttacttttac taatatctat
    36481 taaaaatagt ctaaaataat tttggaaagt gggtaacatt cgcattaaaa ttgcgtagaa
    36541 acacccataa cccaaaccta attttgttaa actaaaccaa gtattaccta aaaaccaggg
    36601 aattatgtac aaaaaagtat ggacaaagtg tcacgaagcc atctgcagca tcaatttttt
    36661 tagtgaaaaa ggcatccgct tcgattctgt taccggtttt aaatatggcc ggtatatcat
    36721 cacaggaagc caggtctata aaatgaataa ggcccgtgaa gtacaaatta cattttttca
    36781 atcagacgga ctctctacaa aagcccaggt aaaaattacc tggaaagatt tcagttcacg
    36841 attggttaaa ggcttaaacg aaaatgtttc ggactttact tttattgaca tgaactttcc
    36901 tcagtttgac aacatccctt cacttcaatt aagaaatgga ggagttgttg ccattggaac
    36961 agaagtagcc ttaataggac atcaatttga ccacaacaac ttatgtttaa aaaaaggcat
    37021 cgtttcttcc aatgtaaaaa agaacgggtt aaactatatc cagatagact catctttaga
    37081 aaagggctat gccggagccc ctgtcatcat tcctgataca ggagaagtgg caggattgtt
    37141 aggacacagg ctaaacgcta tctctgaaag cagaaaacaa atgatggata tcatcaataa
    37201 caacctcgaa ctgctaaaag aaaccgaaaa taaaatacac tttaacaaca ttgacccaat
    37261 ccaggttctt attgccaacc aaaatcaaat caaacggctt gcgcaagaat tgtacaaaac
    37321 agcttccatt aattacggct tagctattga aattagccag gtagctgaat atattgatat
    37381 tgaacagatt gaaaatgtag aatacaggtg aaactcaacc cctaccctag tatgaaacga
    37441 aacgctttgc taaaaaatag caaggcgttt tttattgttc tataccctca aaaagcataa
    37501 tatactttgt tttataagct tcagaattga cagtcacaaa aaaagcctcc ccaatttatc
    37561 caaatccgga aggccccatt tgcatattct attttcttac cttattacag taatagtacc
    37621 atagaaagct tggttacgtt tatcgttgag cttgataata taaaaataac tatccattgc
    37681 agcaattttg cccgattgtt ggttcttccc gtcccatggt acagggtaac ctttggctga
    37741 tttaaacaca atcctgcccc aacgatcgta tatttcaaca gtggcattag gaaattccaa
    37801 atttaaacca ggaatatccc aaacgtcatt atggccatca ttaccaggtg ttattacagt
    37861 aaggatcttc gaagcgtcaa atgaaacatt acaagccaaa attcgaatag tatcataacc
    37921 tgtacaccca taaacattag taacagcaac agaatacaaa cgctcgccag gagttatctc
    37981 aatctcctcg gtgtttaacg gaaacccatc agggccattt aaattccatt gatatgttgc
    38041 accctcatta taagcattta gccttaaaga acctttacat agtattgtat cattacccaa
    38101 atttaccaca ggcaatgggt ggtacgaaac tacaatagta tcttgtttcg aacaattgta
    38161 ttcattagta actttggccc ataccgtttg agctgaattc acaagaaacc aactattggt
    38221 agagtgatca gaccactcta cagctttaaa accttcaact acctcaattg tacgttgttc
    38281 gccatcacaa agcttcaaag tatcatctgt aatgttgtcg aatatatttt taggtgtagg
    38341 gttgatctcc acctgaagtt ctttccaatt tgtaaaacac ccgttcagtt cgccgtaaac
    38401 cgatagctta cgaaaaccag atgtgttcca tgatacataa atagtatcat tcagcgtgtc
    38461 agctgtagca aaaactccac cttcagcacg ccagtgaaaa agcataccat ctggaccact
    38521 aacattgtaa cgcatagcag tgcctttttc gcataccttt tgtatttgct gcgcctgaga
    38581 agttgcagct aatgtaagca atgtaattaa tatgtttatt atctttctca aagcttactt
    38641 ttcataatta atatcaccaa tagaaggtac atcctgaata tcaatcctca tggttggtat
    38701 ctttggtgaa gttgaataat ccacctgata actgtaccct tctttataat atagcatggt
    38761 aatccgaaca tagtgggttc ccacacttaa accctgaatg tttaccgtca gtaccccatt
    38821 atacttgttg tatggaaacg aagattgtac agggccattg tcaatacggt atttgaataa
    38881 atacgactcg ccatctttca gcacatcgcc atcaaaataa acaggaagct ctaaatatcc
    38941 cgtaactggt tttacatccg aaggaggaca gatattgacc gactcctggc tgaaatgaac
    39001 ctgacctcca ccaatagtag ggtcttcttt agcttcaata atagcattaa aaggttctcc
    39061 tgaacaaact ccatcaaaag gaatgacaga caactgataa ttacctactg cccatttttt
    39121 gggcaaacta tccgttttaa cttcttcaat agtatcatta tcaagaatcc acataaaatt
    39181 atcagctgta gcagatccga ccctcaaggt tttatttcct tccttgatag tcaaatgctt
    39241 ttcctgtgct acaactcccg taaaagacag tgttagcatc acaagccaaa taataatatg
    39301 tagtttattg tttctcaatt tagttttata taaccctgcc gatgtttcag accggcaggg
    39361 ctttaatcct tagtatcttt aaatattaga aaatattact aatttcagga gcagtagtta
    39421 cattgattgt atgagttggt tctgtaccag cagaaaacaa tgaggtagtt ccactttcac
    39481 ctgtaatgct gataattctg attgtatgag ccccagcctg agcattagta aaagctgtcg
    39541 ctgcatcaag agaacaactt gttttagctt cttcaaatga aatagaaaca gctgcaccat
    39601 tatcaatagt ataactaata ccagttacat taccaccagt aaaattaaca actgtagcag
    39661 cagcatcacc acctgtaggg tttgttgtag tctgaggaca cacagtttga gatgcatccg
    39721 caaaagttgc accaaatgtc aatgtagtta caacattaat agtagcagta cggttatctc
    39781 cagaacaacc attagcagtt ccataaacag tcaaattgcc agtacttgga gcggctccaa
    39841 aagtaacatt aacagtattt gcagttattg cattaactgc agcgctatta gtaataccag
    39901 caccagaagc gttccaggta taagttacag ggtcagtaat cccattggtc aagtttactg
    39961 agaaatcacg gtttgtattt tgtaaaactt ctatctgagc catcaatcca gaagcaaata
    40021 caaacacagc tattgcacta aataaaattc ttgttttcat attgttgcat tttaattgtt
    40081 tactatttat ttcaattaat atgagattgg tgaagtttca ggtgaagagt aaattgttac
    40141 attttgagaa ggcaatgttg atggaacata atttactcca tttactgtat atgatgtaat
    40201 tctaatctca cgtgttatgt ttgacccaga agtattagta aaactttcat caaaagtaac
    40261 agtaggtgtt agtgtagtaa gagtcccaga aacactatta cttgaaacaa atgtagctcc
    40321 attatagata gaataatata cttcataagt ttgaccaaca gtagctcctg taaaggtaag
    40381 agttgaaatt gggtcgccac cagttggttg gtttgtatcc tgataaggac agatagatga
    40441 aatagcaccc attgtcaatg ttactggtaa agtatttgaa actgtaacat taagagatct
    40501 tgtagggcca ttacaagaat tgctactaat cccataaaca ttaacagtga aagtacccac
    40561 tgtactccat gtatatctca tggtatcaga attagaagac tgaacagtcc cactaacatc
    40621 ccagtgaaag gttgaggaac cagtaccacc agttttataa gtaacagaag ctccagtttg
    40681 aactgtttgt tgtgcattag cactttggtt tatgaaggca attgctgcaa ctgccaaaca
    40741 tgaaagtaaa atttttctca tcgttttatt aaattttttg ttcataattt cattagtaac
    40801 ttataggttc agtaacaggt gtccccaata ctgtatatat atgtgtattt tgtgcatcaa
    40861 tagtcataat agccccgtgg cttgttgcat tagtaatagt aatggtttta ggtatgtctg
    40921 aagtagtgga attaaccaaa atatcatctt cactacctaa atcaatgctt gtaccactgt
    40981 ttattgtcct tgaatgagat gtagttgttg ccccaactgt taatgtataa gttataacaa
    41041 ccgggtaaac aatatttcca gaaaaagtaa ggttcaacgt cagatcagtg ccagtagccc
    41101 cagaacaatc gtcactactg gcagaagcaa aagctatgtg agcattatta ggcaccacag
    41161 caactgtata attggttata ttccccgaag cacaaccttc ctgagatact tcttgcacac
    41221 ttactgtata agtgccagat tggagccatg aaacagcaat agaattatca tggtcagctg
    41281 tactgtatga aaagtttgta ccatttgatc caccagtaat attccaatta aatgtactgc
    41341 tgccagttga agcattttgt gatgttgtat aattataaga tgaaccagct accacagtat
    41401 ggttttgtcc ataagcatgc cctaccaaca tagtagccat aaacagtatg gctgccaccc
    41461 tggtggttac attttggaca atatgtagta aaaagttcaa catcaatttt ttaannnnnn
    41521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagtat atacaccgtt tcggtataat
    41641 tgataaacga taccacttgt agaaccagcc aatataacaa tccctgttcc attgcaataa
    41701 tttgtacctg aaactgtgta tgatgttaat gttgtacttc caaaatatgt accgtaaata
    41761 tcagggcatc ttacagaaaa atctacaaaa tcgcccaccg gacaatcggt cgaagtatta
    41821 ggtcctgtat atgtagcgtg attacaaaca gcctgagatg gaacaggagc aataagatta
    41881 tatgaatttg ttcccacata caaattacca tctccattgt atgaaaccaa atgtccttca
    41941 aaagtacctt gtactgcaat tacgccatca ggtgtaatat taatatctcc ggaagaagaa
    42001 tagttaccat gaacaataaa agggccacca tttatattga gagttccggc accagtcata
    42061 ttaacattac caaaaacctc aataagcttt gaacaagcaa tattggcatt tgctaaatta
    42121 agatttccgt atatttttat ctgacctgaa ccttgagtag tcattgcaca gttgccatta
    42181 attgaaacat ctccataaat ggataacacc ccactgttaa tttcaaaatt atacacatta
    42241 ccatcagtaa cagtaatatt tgaatttaaa tcgatgtttt tatcgccacc ttgcattctg
    42301 attaatacac cgtgccctga gttaactata ggaggaatat ttccaccatt ccatgttgca
    42361 gcagaagacc aatcgccagc agatgcagcc tcataagtag tttgagcatt aaatggcaga
    42421 taataaaata ccatcgtcaa aacgacaatt atctttattt tgagggtact aaaaaactta
    42481 tataccattt ggtagatacc ttatagttag tgtagtgttc agcaaatata ttatattttt
    42541 ttcaataaac taaactttta tagcaaattt acactaacca caattttgaa gtgactaaca
    42601 acaaattttc ttagattaag aataaaacca atacgtcaaa gaactaattt aattagctaa
    42661 tttgccaatt agctaattag taaatatttt taatcgtgcg tcatcccatt ggtagttggg
    42721 gtaacatata catttatcgc ttttgtttgg ttagcagggt ttgtacaacc cggcaatgct
    42781 gtaatctgca attgcagagt aacagaagca gccgtatttg atgcagccct gtaaacagga
    42841 actatagtat tagcaccatt tattatagcg ccaccacctg aaacaacaga ccaattaagt
    42901 gcactataat tggtaatatt aaccccagta acatttaaat cattagtacc acaaacagat
    42961 agcacccctc ccataataat aaccggagca ttgtttacgg tcactgaagt aatggttctt
    43021 ccgctactaa tacagtttgt agcagtattt cttgcctcgg cataataagt acctgtcgtt
    43081 aaagctgtac ctgcggccaa tggagtacct cctgtagcag atgaatacca atcaatagta
    43141 caaccggtat caactgtacc cgtcaaatca gcaactgtag cccctgaaca gaaactttgg
    43201 ctaacagtag aagggattgc aggagttgcg ctccaggtaa gcgtccttgt atcagtagcg
    43261 ttggtacaac ctccagcacc tgtcagggtt aatgtaactg ttgaagttcc tgatgtagca
    43321 gaaggctgga aaatagccag cgccgggtta gaaatatgtt gtgtccacgt tccgcctgct
    43381 cccgaccatg ttattgccga ataattaccc gaggcagtag cgccagtcat agaaatgctc
    43441 ccatttcctg aacaagaagg aatattatca ccagcttcag caacaggagc ctggcttata
    43501 gtaacagaag tacttcctga agcctgacaa gtattgttat ctgtaaccac atagttcagc
    43561 gtataagtac cgcctgtatt gttattgtag ttagtagatg cagatgtagc agcagagaaa
    43621 gtaccaccat aagcagcagc ttcagtccat tgatgagtat atggagatgt accaccagcc
    43681 acagtggcca gcaaagcaat gttagttccc gaacagcccg aatatgttgc agggatagaa
    43741 actgtaggag ttggattaac agtaatacta taacttgatg caggagcagg agtactacat
    43801 ccattagtag cccttacggt taaagtacca gaggttgcag tagcagaaaa atcaatacta
    43861 atggtaccgt ccacaccagg agctgccgaa gtaagatatg cattaacggg tgttgctgtt
    43921 ccatgcacag ttgctcctgt tcctgagaat tcccatatat aaccggtagc attagttgta
    43981 gaagccacag taaatgtata attgccaccc ctgcaaaccg aagctgggcc agaaacactt
    44041 cctgttgtgg caggagccgg ggtattagaa actacaatac tttgcgaact tgttattcca
    44101 tttccacatg cattaacccc tctgacagta agtgttccag atgtagctga agcactaaag
    44161 ttaatattag gagaagctgt agtgctattg tttatggtca ccccagagcc tgaatacgtc
    44221 caattgtagc tggtagcatt agcaatagcc gcaacactat atggctcatt caaagtatta
    44281 gtacaaacag cagtacttcc gttgataata cctgcatcat cgggaagagg attaacagta
    44341 atagacaaag cactacttag ccttgagcag ttgttattag tcacaacaac atctaccgaa
    44401 gatggattca acgtcaatgc attagtatta aatacgttag aacttccatt ctgcacnnnn
    44461 nnnnnnccgg cattggccgt aaaagtaata gctgctcctt cacaaacagc agtattactg
    44521 gacgaaattg tagcattagg cagtgcgttg actttaatta cagcatcaat attacccact
    44581 aaagtaccag gcaaacctcc ctgagacaca gattgcaaca catacgtata tgtattagct
    44641 gtggctgtag gaacatttat agtataaggg cttgatgtta cattagcgtc aggctgggta
    44701 acaccattga ggctataacg caacacccat ggcccggtgc cactaagagc aaccacaaca
    44761 tttgccgttg cattttgaca aacaatagca tcactactgg tgattgtagc agtacaatta
    44821 gaaatattag caacagaagc caacgtaaaa acagaatatg tgccaaagtt tactgcattt
    44881 tttgtagtaa tataacctga agtagcagaa ccagtgatta tagaaccagc gccaaccact
    44941 tcccaacgac tgctaccaga gttccatcca acaatacgta agttattgat attagaaatt
    45001 gccccggcta tgtcgcttga accatcaaga taaagcctga cttttccaga agaagaggta
    45061 gaaggagcga aaacactcca gtattcattg ccactaacct taactacagg cgaaaggcga
    45121 cttgaagtat cataacctgc ggtatgggca ttatcataag tataagaagc gctccaatct
    45181 ctatagccag aagggccatt aacggctaat aaagccatcg ccccaagatt tccattatta
    45241 ttaccaatgg ggaaagtaaa gcttcctcca ttaatcatca ctttggtaag cgttccatta
    45301 acaaaagagt tgcttaaacc tttagccgga gaaactgtac ccgaataatt aatgcgtata
    45361 ttattggaac cagtattaat caaaccggca gttaacttca atgtgccatt aatttcaaca
    45421 tcgttattga gggtaagtcc attaatatta gcaatttcaa gacccttaaa tgcatttgtc
    45481 ccggtaaaag aaccggtaat attttgaggg atagaaccgt taaacaccat ataagaattg
    45541 gcaccggctt caaaattacc agatattcta atcaaatctt tgtgtaagta tattttccgg
    45601 ttatcataag taacagcata taacaacgct gtcccgttaa tggtaaaatc tcccaatatt
    45661 tcaaggttat tgtttggaaa tcgtttatca ccgcttcccg acaatactaa atggtttaca
    45721 gatagaatat ttccaagaaa atcataatta tcagcccctc caaactctat ggtaccgcca
    45781 gcagtagaga aaaactcgtc atataccccg gcagaaatag aaccttgctc aaggtacatc
    45841 gtacctgttc ctccaataat tccatgacga tgaccggttg taaccccaag gttcagcctt
    45901 ccataaatat tggtagcata ttctttaatt ccatttactg aaatagttat agtatgtccg
    45961 gcatttatct tagtgatagc gccggaaggg ccattagtaa cattagggga ccataccgtt
    46021 gcagtttgcc aattacctga agataatgtt tcataattca ttacattatc agggatagca
    46081 ccatttttag tagcatcaac agccccggca gtaaaatcag cattaagttc agcatcacct
    46141 acattagcaa aagaaaaact tagttcattg tttatttggt ccacttctgc ccagtcgcct
    46201 ttgacccaat tgccaacacc atcattttgc aagctggcag gaatataatc attaatacta
    46261 taaggagctg taaatttaat atcagaagga agatatttca ttttagctgt tccgctgaaa
    46321 gcagaaatgc ctgatgtttt catagtccaa tagtattgaa gtgcattatc agcatcaaca
    46381 atggcaacac cagtttctgt atcttcaatg atagaaggat gaatttcgtt agcaggttta
    46441 actgtaatac ttcctgtact attaccattt gcagtaatat tgagggttac aggggtatat
    46501 ttaccatcgc aaccaacagg ataaacaaaa gttattggtg acgctgtggc agggaaaacc
    46561 tttctcacac cattatcagt aaatgaaata tttgtttcaa tcatgttgat gtctgaaaaa
    46621 ggcgaagctt gttcaatagt acagttcaat ccaagatcaa ggaggttttt tccaatatta
    46681 aaaatacctg cctgcatcct tagtgcattg gtaattttga actggtttgc caccggaaca
    46741 tttacaccat gactgttatt aatagtcaac ttaccaaaag tgccattgcc agttaatgtt
    46801 tgaggttgaa ccccattcat cacaatacca tttccaaggc cgccataaac atgcgtccca
    46861 tcattgtagc aatcaccttt gactgaaata gtattgccat tatctgttaa agtaccagtc
    46921 tgaatagcta aattattaag aattgtaaga ggtgtattat tagctgtcaa tgccaggtta
    46981 ttagatgctg tttttgtcaa gttataaaaa ttgagaggtg ttccattgcc agtaatggtt
    47041 tgagttaaag aaccatcgaa ataaactgtg ttgccattag gcacaaaagt tccagctaca
    47101 ttcatgtctc ccatcaaata catgtcttta ccattagcat taaatgttgc acccgcgtcc
    47161 acctgaagaa ggtttgtaat agtggccgga acaacatcga gccgggctgt aggactgtta
    47221 aagccaaaaa catgcaattt aggcaactca tctccggcat aaatactaat agtttggctt
    47281 gccggagtca aagaaggatg cccaatatga atattagcag cagtactaat attgtttaca
    47341 tccgggtcaa aatagaatga agcaatggta ggattggact gttgacgaca aatataaagg
    47401 tcaccaccag acatgttaaa agaacttcca gggttcaata tttcaaaaac accacgattg
    47461 tttactgtag ccgaattttc accgacaata acagtacctc cagactgggt ataatccaat
    47521 gtaccaatgt tagaagtcgg gctgcgacgt atttgggccc caacagtcaa tgaaccggaa
    47581 gatatttgta atacagagtt cccggaagac gaatattcaa ttggattatt cccgccggcc
    47641 atatttaatg tacctccggc aattcttaac aaaccatcta gttcaatacc cgaatttcca
    47701 ttagcattgg ctgtaccctg tctaatttct aaccctgctg tagaaggaat tgagaaataa
    47761 tcgtagcctg tagtcaggtt aatattggta gctgcattat tcatgataaa caaaccattc
    47821 ttaagctcca aagctttagc gacccctgca cctgatgttg gcccgttaag gttaaagttg
    47881 caattaaact ggactgtaga agcaatgctt gaacctttat cgataatcaa gcggtacaag
    47941 ttggtaacgt taccagcagt agggttagtg atggtataag tagcattctc tccacctgtc
    48001 atataaaggt tgatatgggc aaggctcccg gcattaccta actgaaggcc atttggcaca
    48061 gctggggttt cgtcagcata aggtttttca tctatatata tatcaccatt gacttttaat
    48121 gtatgttcaa gcgctattcc tccattaggg tttctgacat tgatgatagc gctggtattt
    48181 ttcatactta taccacgccc aacggttata gtacgctcat ttccagaatt ttgataagca
    48241 atttcagcgc ctccatagct tctatttccc tgcacacctg cagattcaaa aaatttaagc
    48301 gtccggccta tattaatgtt acctgtagca cccgaagaaa gcacaaggtt aatatcgcca
    48361 agaatttcaa aatctccggt agtattaata tctctggcaa aagtaatatc atggttattg
    48421 ttaccccagc catcattaga aatcataaca ttggggaata atggagcggt attttgataa
    48481 gtaaaaggag tttcaaaatt ttcataaagc acataagaag aatcttcgcg tgcaaaatcg
    48541 ccaatatcca tcaaataata gcttgggtcg ctattatcac ggttccagaa cataccctcg
    48601 ccccttacat tctttgcaga caactgtccg ttataattat tgatacacaa tgtaggccta
    48661 aactggaaat ttgagcggta tttacgtgct accgggtttc ctgcaatatc tgtcatttta
    48721 taaaaaacca cttcggcagc agcatgggtg gtattatcaa tccaaacgcc atgaggcaaa
    48781 cctcgttgat caatattatc agtagcagtt ccaccattag caggatttcc gtctttttga
    48841 acatcatcat aaggcaccca gccaatttga gctacatctc cagcttttgg ataatcccca
    48901 acagcaacag caggctgacg gctatcatga ggactatata aaggattaag aatatcgctt
    48961 cgggtccagg tcaaattgtc ctgccaacga ggttcgcggt tagtaatatg ctgagtcccg
    49021 tcattatcgt taaccccatg atcacgagta taataaataa gcacactacc agaaaacctg
    49081 gtagataccc cggcagtaaa attagtattt tcagctacaa agccaacagc agcattagta
    49141 aataagccat tggtgctggt cccgttaaag gtaatcattc ggctgttatt gccagcgcca
    49201 aaatttttta caatatcatt gtctgtaagt ggttcaaaag cacgggtata aggagattcg
    49261 tccaatactt taccaggcaa aaagttgttt tcattattca ataagtcaac attagccaca
    49321 ccagtattgt ttcgataata aaaacgatac gagacggtag gatttgtatt gaaaccagaa
    49381 cggctaaccc tccagtaatg ctgtaatacc tcacctcctg taagattaga tgtaggcagt
    49441 gcccccctta ccggacgtac agtaacataa ccatcatctc cggcagctaa atgagaaaca
    49501 accatttgtg caggacgaaa atataaagta gtaccgccat taggggtaaa accaacaggg
    49561 aaagtcacag gggcacggtt attaagcctg tttacctgat tttcagcaag tgtttcttca
    49621 taaatacgta gcgttaagcc tccatctccg gcattcccat cagtaaatat catagaatta
    49681 cacccggcag ccaaaccaga gttaacaatc ttgagattgc ttgaatttgc aacatcttca
    49741 aaaaggttag tttctaaatt ccacaaatcg tccactttca ggttaaaatt cttgagataa
    49801 atcaaccctt tgacaaattg taacctttca atataaatat cactggtaag gctaacgata
    49861 gtcggaggag aaacattaaa ctgaacgtta ccgaatatgg ctttatttga ggtattaata
    49921 gttaacccgg ttttatcggc aaattggatt tgagcagaac ttggcgttgt cggggtattt
    49981 gtacccatac ggtcatagtt gtagatatta ccccaggtac gaatggtata attcccctga
    50041 tcaagagtac cagacaaaac agatatattg ccattgacat ccaaaaggac agaaccatta
    50101 gtcctggcag caggggcaga taacctaata gcattgcccg ttgttttgtt tactaccatt
    50161 ttcccaaatt ctaattcggc agttgcatta ccaaagtaca attcttcatt ggcagaagta
    50221 ccagcagttt gattaaaata tgtagtattt gatgttgtag gctgatttgc aatagagtta
    50281 tatggtgcag tacctcccag aattgggatg tactgagctc cattttctaa ataaaacgag
    50341 cctcctatat aaacatcatt tacattggtt gcactggtaa ccggggtaaa ccggataaca
    50401 gggtaaccgg cgcctccaga ttcggttccc cagatgcgaa aatcatttaa gacttttaaa
    50461 ggttgagccg caagatcgac atcagtagtt ccaacatcaa ccccttcatt tataatgaaa
    50521 tctctttggg cacctgatgt atttctaaat tcaaggttcc aaaatggagc gcgtgaatta
    50581 atagaagggt tacgagcata acttgcttca ataatcacag tacctccggt aactttaata
    50641 ttttcaggat cagaattaat aaaaatagta cccttagcag atgcattatt gactcgtaat
    50701 gtccctcctg acatattgaa aatactattg ttatatgtca aacaaaaagt gtaataatca
    50761 cttgtataag gagttcctcc aacatttact tcaccaccag tttggatata acctcctaaa
    50821 tgagcagcac ccaaaaccga agtcctaatt tgattcacat taacagtacc tccatcgaca
    50881 aggatcaacc cgctttcgcg aacagtaata ccacttgcta cttttgattc taataagcca
    50941 ctggtaattc ttatttttcc gtaagggact atagcattac caatattctc caatacagta
    51001 cccccatcta cccatagttg ggcagcagta gaaatattgt aattaccaga ataagaaaga
    51061 gagctaatgg taacaccatc tcctatacga acggtacctt taattaaagc caatgcatta
    51121 tcattggtaa ttaactggtt aatgtttgga tgaccttgat tagtataacc caataaataa
    51181 aaattagttg gagcagtagc atcaatattg agaatgtaag tatcatctgt acctttatcg
    51241 attttgatgc gataaaaaat tgaaggccca tcacagagta tgctttgatc tgcattagcg
    51301 ttcagacagt tcaaatcaac aataccatcg gttgcctgag ttgtataata cgtatctaca
    51361 ataggggcgg tacgattggt aaaagccaga tgtccatgat ttttgaggtt gccatataaa
    51421 tcaagctggt gacgggcgtt ggcagaacct gtaacgatat ttccggctgt attaacagta
    51481 atatctttgc gaacaataat attcagattg ttagtagctg tagcattatt aatagacaat
    51541 gtcccggact taaccaacag cgaaccgttt acagtcaggt tgttgagcaa agtaagggtg
    51601 tttgtcccca tatttacctc aacgttatag taagtagttg ctaaagtaat atcacgcgaa
    51661 gttccataaa attcgacagt accttcacct tgtccggctg taataaaatt agtagcatta
    51721 ccagaaggaa aattatttcc tgcaatacga atttttcctg aaccttcaat atcagtaaaa
    51781 ctatgtccgg caacatctct caaatcaaga acgccattaa ctgttaattt agcattaact
    51841 ttagcccctg aagatacggt aattgtacgt cctgaaagga taacgacttc atcatacata
    51901 ctggttgtag aagtagcagg cgtaaacccg ttagggttat taggctgaga gccggaagga
    51961 tctagcgtcc aaacagaagg atcgtcccaa tctccacttt ttaatgaata aaaaatccgc
    52021 ttcccggaaa cagagccaga ccaggtagca tcaataaaag gagacgaaac cgaagccata
    52081 gggttgacac ctgattggct taaaccggat ggcacaatcc acgaacccat atagtttacg
    52141 aacaagtcat aagccgtagc cccgccagtt cggtcattat tatcataagc aaaactaaaa
    52201 ttggccaaaa cagggcctga aataccagta gaagtagttg tccactgacg gttcagagga
    52261 ttagagcccg gaatacccgg aacaacctgc gaattagccg tagcaactgt gatagtcgaa
    52321 ttaggggcaa ctgttcctga taaactattt aagctgacag ggacatacct tgtacccatt
    52381 cctatgggaa atattgaaac taattcatct acggtagagc cacgtttgat aaagcgcccg
    52441 agtcctgact ggttgacaaa gctcgtgagg ctgcccccgg taactttacc acctacgata
    52501 aggttataat tgcccaaatt aaggataccg ttggtaagaa taaggccgcc attaagtgta
    52561 gtatggttag ctaaggcctt ggttgctcct cctccaattt cgagtatccc ataattataa
    52621 gttgctatag tttggtcagc agcactttga taagcaacgc gacttgtagc atcaagcgtt
    52681 acagtagaaa aattggttgg aaaaattaca gcagtagcat ttgtagtacg gcctaaagta
    52741 agcgcagtac cagctcccaa agtaaacgag cctccagcag cagccctgct aatttgataa
    52801 atatccgaag ctaaagtagc tgtacccgaa acgttgagat tgccggatat agtaatattg
    52861 ttagctatcg ttttaacgcc agagcccgaa acggtaagat tattatagga atatactgaa
    52921 actgtttgat ccgacaatcc actataattt acagtaccag aagtagtcga ataagatgca
    52981 ggagtaaatg ccccttctat ataaatagtc ccatttccat ttactgttat accagtacca
    53041 atattaccag ctacgtacaa gattcctgca acagaaatag tgccagttcc ggaaattgaa
    53101 ccatcaacat ataaagtttg accattactt atggtaattg tattattact attaaaaatt
    53161 acattattta catgatatgc aggcgtaaca ttaagcgtta cattattttg aatataaata
    53221 ctaggcaaac tgttgtcaac acccggataa tttgcagtat tgttccaggc tgtgtttgct
    53281 gcattcctta tttgccaaac atttgcacca ttccaatttc cattgtttct tgttcgataa
    53341 tctcctgcag actgagaaac agcatcaaaa gcactaaaga aaaatgcaat aaataagaat
    53401 aaaataacac gaaaagcttt accttttgag tttactctat acatgtaaaa ctttaagccg
    53461 gataagttat taatattttc caatttgaat ttgattaaag tcatggtttt caggtgacaa
    53521 atatcacctt tttgtacgaa ataaaataca gtctggttta aaatttttga tgttagataa
    53581 aataaattac acgaacgctt gtttttctta gacaaaaaaa gtaaattatt tttgaaaaaa
    53641 catgaaaaac catactaata ttactttttg tcattttgac aaaacttaaa agattcattt
    53701 tcaaaaaaaa tgaaaatata agaaatataa acatttctat tttttatggt tcttatctaa
    53761 caaatgtaaa attagcctcg cctacctcct gcctgaggcc atagccatca agacaggaaa
    53821 tgtatcagta ttcaagccca gtaagggctt catattggta gaaaatacgt tttaaaataa
    53881 accgcaccaa gcacttaacg aatgatgcgt ggtgcataaa aataaaagct gattaaatga
    53941 gagtcttcta ttgtgcgccc aaaaaaggaa tgctcatttt ctatcatact tctggcgcac
    54001 tttatttgag cattcttgaa ttctaccaat atatagccct tacagggctt aaatacaatg
    54061 tattattttt aagtacacct tagcttgacg gcgatgcgac agaggcggaa gaagctacag
    54121 attctaatta agaatacatg caaaataatt tggcaaaaat ttttaacttg ccataccgaa
    54181 gtaataatcg tatctttgca gaagaataca tgtcagcatg gggctgaccg gttttgacag
    54241 caatggaaat ggacatgtaa gcatgcaggc tcacgcaaca acagctttta cagaggttgc
    54301 aaaatataga aggcgaaaat aattttgctc ttgctgctta atcgaattat agtagattag
    54361 ctttatcccg gcccaggtgc cgcggacaag acgtcgctcg gggagcaagc ccatcggtcc
    54421 ctggtcaagc ggcgctcgtg agaatgggat agttggtatt aagctccggg gtaccaatga
    54481 aacaaaggag ataagggctg gttaggctgt ctgttgccgg accaacttcg aaaaccaaga
    54541 accgactaag catgtagaaa gcatctctgt ttattgtttg gacgcgggtt cgactcccgc
    54601 cagctccaca aaaagcctct cccctatcct atccaaagga gagggccatt atagcaaatt
    54661 cttgttattt acggttcatt cgattacatt tgtttttgta tataacttaa acaaaaagta
    54721 tccataaaag aactatgtaa atgtaaaatt ttaataatat gagaagaata attatggacg
    54781 atgagcccga aatggaacct caatcaaaga aatcagcatc gatgaaaatt attgatttga
    54841 aatttctcga acaaagaaaa atctttctgt ggggcgttgt agatgacgac tctgctcaag
    54901 atatcgtgag caaactatta tatttggagt cggtaaaacc tggtgaaaaa ataacctttt
    54961 tcatcaatag cccgggaggc atggtaacat caggtttctc tattctggat accatgaacc
    55021 ttatatcttc gccggtgtca actgtttgca tgggacttgc tgcttctatg ggatctatat
    55081 tactttcggc cggtgaaaag ggtgaacgat atatatttcc attaggcgaa gtgatgatac
    55141 accaaccaag cgtaggtttg ttgcaaggta cagctgccga tatagaaata caaaccaggc
    55201 aactgctcaa aaccaaagag attggggctg agattttggc taagaattgc aaccagtctg
    55261 taaaacagat atacaaagac tttgaccgcg actattggat gaatgcccag gaatctatca
    55321 attacgggat tgtggacaag atttatgaga tgaaataaac ttgtaaaata accatttgtg
    55381 gttatttttt caagtttggg gtttgtggta ttctatgatt ttcatatttt tatagcatat
    55441 tacaaacccc aaacttttac acaaatgttc acctggaaaa ctaaaggcgg ctataggata
    55501 gtacaattaa ttggcggcag aagcaatgtt tttcttgtat tttacggcac caaaaaaata
    55561 atggttgata ccagcacgaa gtggaactgg aataaacttg acaaacgctt gagattatac
    55621 gctataaatg aaatagacta tctggtttta acccattcgc attacgacca tgctgaaaat
    55681 gcccgtcgaa tccgtgaaaa atatcatgct acagttatcg tgcataaaaa agagtctcac
    55741 tttttgctgg gtaaagaaaa cattgtccct gccggaacaa acttttttac ccgtttttta
    55801 gcatcatttt ttgggaaatg gttcttttca ttacgaaaag taacctgtcc tgtggatatt
    55861 gtagttgatt caacatttag ctttcacgat tttgggttca aagcttatct cattcacact
    55921 ccaggtcatt ctgaaggctc tatgagcctt attgttgatg atgaagtggc aatagtaggt
    55981 gatgcaatgt ttggtgtatt ccgatggacg gttttcccgc cttttgccaa caatccccga
    56041 caattagtag atagttgggc cccattgctt aataccaatt gccaggtctt tcttccatcg
    56101 catggtaatg caaacacccg ggatatggtg ctgaaagatt atgaaaaaag aaaaatagct
    56161 tagctttctt atccataacc taatcactgt tttacggtat tcaatttgcc atttatgcgg
    56221 tattccaagg ttaaagcaat cttcataaca ttgcataaac cattaataat taaagctatg
    56281 gcagatttag tatttagtgt agaaggaaaa gcagaatcgc aaacacgcat tactgcaaag
    56341 gcaagacaat tcagtattgt tgttgacgaa cctccaaccc tcggggggca ggatttaggg
    56401 gcaaacccgg tagaatatct gctggccagt tatgccggat gtgtcaacgt tatggcctat
    56461 cttatagctg cagagttgaa catcaaactt gataaactga ccattaaggt tgacggcaac
    56521 atcaacccca atcgtttgtt tggaaaatcg tttagcgaaa gggctggttt taagcaaatc
    56581 aacctgactt tagaacccaa aacagatgca tctccagagc tgataagcaa atggataaaa
    56641 gaaattgaga accggtgccc tgttaatgat aacctttcga acccaacccc tgtttcaata
    56701 aagttatcgt aattttaaag cttatattaa tgtcgtttag ttaagtataa aaaaaacgcc
    56761 gacaggcctg aaagctgtcg ggttagtttc acccctgacc tgacagcgtt cgcagaacct
    56821 gtcaaagtct taactaaacg acattgaatc ttatatattg aaagaaatat aaaattaact
    56881 ctaatgttct caaatcttct atgtccaaga tagcatatta ttctttagct catcatttac
    56941 tggcccaatg gggggcatga gatttttatg ttaatatgca attaaagatt atatgctttc
    57001 ttcaatgcct gctcaatatc ggctataatg tcattggcat tttcgatacc tacagaaaaa
    57061 cggataaggt caggggcaac tccagcggct attaactgct catcagtaag ttgccggtga
    57121 gtatggcttg caggatgaag gacagatgta cgggcatctg ctacgtgagt aacaattgct
    57181 gccaactgca aactatccat aaaacggata gaattatccc gtccaccttt aagtccaaat
    57241 gtcaaaacac cacatgaacc atttggtaag tatttcaggc caagctgata atacttatca
    57301 cctggcaaac cacaataatg cacccatgcc actttatcat ttgattgaag atattcagcc
    57361 actttttgtg cattctcgca atgacgtgcc atcctcaaat gaagcgtttc taagccaaga
    57421 tttatcaaaa atgcatggtt aggcgattgt atcgagccca aatctctcat caactgtacc
    57481 gtagcttttg taatataagc cttattccca aaacttttgg tatatatcag gccatgatac
    57541 gaatcatcag gagtggtaag ccctgggaat ttgtctttat tggcatccca gtcaaattta
    57601 ccactatcaa caatggctcc tcctacagta ctggcatggc cgtccaaata ttttgtggta
    57661 gagtgaacaa caatatcagc gccccattca aaaggacgac aattgattgg agtagcaaaa
    57721 gtgttatcaa ctattaatgg aacgcccatt ttatgggcta ttttggccat tttttcaata
    57781 tccagcacgg ccattcccgg attggcaata gtttcgccaa acaaggcttt tgtgttagga
    57841 cgaaatgctt ttacaatatc ttcttcagaa tcgtcctgat ttacaaaagt aacctcaata
    57901 ccaagttttt tcatagttac agaaaaaagg ttaaaggtcc ctccataaat agcagaagtg
    57961 ctaacaaaat agtcaccagc tgaacaaata ttaaaaatag cataaaaatt agcggcctgt
    58021 ccagacgaag taagcatagc cccaactcca ccttcaagtg cagcaatctt tgaagcaaca
    58081 gcatcattgg tagggttttg caaacgggta taaaaataac cggcttcttc caaatcaaaa
    58141 agtttggcca tttgctcgct tgtttcgtac ttaaatgtag tactttgata aattggcaat
    58201 acccttggct cgcctttttg cggctgccat ccgccctgta cacagattgt ctccctgctc
    58261 tgcttgttca ttttattctt tctttttgtt tcttcgttaa aacttaggtt taaagcaaaa
    58321 cccttttatc aattcagaaa caaaagtaac aaacaatttg ttattcagag tacgaatttc
    58381 atcaggcttg actataaagc aacagaatca aacaaatacc tgccctattc aacgacttca
    58441 gggcaaaaag ataataaaac aaagggccat aaaatagata tttctacatt acggccctcg
    58501 attatacgtt gctgcctttt gaaaaagaca attttagaaa aaaatcagtg ttgtctggta
    58561 aaagtattaa tgtagggcta aagccctgca catataaacc tataaatgac tacaataaat
    58621 aatcgctaaa aacaatctgc gaccgtttta attggttttc cggacaacag tgaaaaaatt
    58681 aacctttaag tttttttaaa gcttcaagtc tttcctgatt ttttctaata ttttcttcaa
    58741 gtttttgaat ttctttttct ttgatatttt caagaatttc tctgattttt tcaatttgct
    58801 ctaaagaaaa atctctattt tcaatttctt gtttaagaaa tgaaatgaaa tctttttctt
    58861 ttctgatata ttttcttttt gttggcttgg gcaattttaa atcaacaggg ttttcagttt
    58921 ttccttgaat tgtattaact gggttttctg ttttctttgc agcaatattt ttaaactttg
    58981 ctatcttttt agtagctggg gcaggctttg caactttgtc ttcaagaatt tttcttattc
    59041 tgggcataaa aaataaaaat taactgtgaa taatgcacaa atatataaat tatttaattc
    59101 taaacattta caatcaactc cccaaatagt aaatttacaa acgttaaagt ttgtatttcg
    59161 ttcatggcat tgcacaaatt gaaggtttgc ccaaagaccc gggagtaaaa gatttaagag
    59221 cccaaagagg gagaaaaaca caaagttgct tgcttttgtt tattggttaa tcaataactt
    59281 tttttatcat aacgataccc gaaaaacaat ctcttccaca ataaatacca tttttattta
    59341 acaaaaatat ttgatagata ttttcttgtc tggcaaaatc tttcaaaaac aacaagtttt
    59401 cgggtttgta attggctttg atggctttga ttatacgttt agtgtttttg tttgagtaaa
    59461 atttgacaga atctgtattg aattcgtaaa atccaatcat cattaatgat tgagctttat
    59521 tgaagtgaaa aacaaaatta catgatttgt tattgacaat gctatcaaaa cttttttttg
    59581 ctatctcatc ggcttttatt tgtgaaacag atttgtttaa tgaaataact tcgctcatat
    59641 atgcgctata taaagcattg ctttcgcaac atatttcttt tgcagtgttt gttttggtat
    59701 ctacagctgt aaatactaca aaatctgaag aacacgaatt gttataaaaa gttttattga
    59761 tatactttgc cctggcagaa tcataacaat tgttgcagtt taccaaaagc agcgcatgga
    59821 aaaagaaaat aaaaatttta acgtgtttca tagtagtgtt ttttatttgt gttttataat
    59881 caagccaaaa gcattttaaa gggcaaaaat attaaataga cattaataat gaaaacccca
    59941 tgattctgca aatcgcaaac acgatgatgt ttattgtggg tgttctcccg ccaaagacag
    60001 gacaagctat cattccttaa aaattaaatt ttattatgaa ggaataaggt acatttaaaa
    60061 taaaatggcc ctctgcaaaa acagaaggcc atttttattg cgaacctgac ggttcattat
    60121 cttcggctaa agattataca agaccttgag ctatcatagc atcggccact ttgataaagc
    60181 caccgatgtt agcacctttc acgtagttta catatccgtc tttttctgaa ccatatttta
    60241 cacaagtagc gtggatgtta cacatgattt ggtcaagttt agcatcaact tcttcgcgtg
    60301 tccaaggcag gcgcattgag ttttgggtca tttcgagacc tgaagtagca acccctccgg
    60361 cgttagcagc tttgcccggg ccatacatga ttttagactt taagaaaact tctatggctt
    60421 ccggggttga aggcatatta gcgccttcgg ctactgcaat acaaccattt ttaataaggg
    60481 ttttagcatc ttcttcgttt aattcgtttt gtgtagcgca aggaagggct atatcacatt
    60541 taacccccca aggagttttg ccttcatggt aggtagcgct tttgtatttt gcagcatatt
    60601 ctttgatacg gccacgtttt acatttttga ggtccatcac aaatgcaagt ttttcttcgt
    60661 tgaaaccttc agggtcatgg ataaaaccgt tagagtcaga aagggtaaca acttttgccc
    60721 catattgcat tgctttttca actgcatact gagcaacgtt gcctgaacct gaaataacaa
    60781 cagttttgcc tttcatgtcc atttttctca tcttcatcat ttctctgaca aaatatactg
    60841 taccgtagcc agtagcttca ggacgaatta ggctaccgcc ccagccaagg cctttacctg
    60901 taagcacccc ggtaaattcg ttacgcaatc ttttatattg gccatacata aaaccaattt
    60961 cgcggccacc aacgcctata tccccggcag gtacgtcagt atctgcgcca atatgcttac
    61021 aaagttcggt catgaagctt tggcaaaaac gcatgatttc gttgtctgat tttcctttag
    61081 ggtcgaagtc ggaaccacct tttccacctc ccataggaag tgttgttagg ctgtttttga
    61141 aaacttgctc aaaagccagg aatttcagca cacccaggtt tacagttggg tgtaaacgca
    61201 aacctccctt gtaagggcca atggcactgt tcatctgaac acggaaccca cggttgattt
    61261 ggaaatttcc ggcatcgtct aaccatggaa cacggaaaat gataacacgt tctggctcag
    61321 ctatcctttc aaggatttta gcagctttgt acttagggtt ttcttcaata aaatccataa
    61381 gagattcagc aacttctttt acagcctggt gaaattcaga ttccccaggg tttttagcaa
    61441 taatttttgc catgaattca ttcaatgcag catctgtctt tgccatattt tttgtttttt
    61501 aaaatgttta aaattttcaa tgtcccaaat gtaatatggc ctttggattg acaaaaaata
    61561 tggactttac tttacgtgtt tcacgtatgt ttttcagtaa ttttcttatg caaaagattt
    61621 tatatttcta tcaaattatt gcgtatggca aattttacca tatctacggt ggtctttagg
    61681 ttgagttttt gcataatatg gtttttgtga gtttctacgg ttcgaaggct gataaatagt
    61741 ttctcggcaa tttctttgtt ggtataattt tcgccccaga gtttaagtat ttctacctgg
    61801 cgggcgctaa ggttcccgat ttcagcttta ggcatcttgt cttcgctttt gaagcgggta
    61861 atatatttat tgataagtaa atgtgtgatg gacttgctat aatagtcaaa accattacga
    61921 atagtataaa tagcttcaac catctcgttg cggtcagcct ctcgggccaa ataacctttt
    61981 gcacctgcct tgatagtttt caaaaccatt tcgtcactat taactgctga aattacaagt
    62041 atgcgtgtct ttgaaatggt aaggtttatt tgcgagatta ggtttaaaat agcagtatca
    62101 aggacatgaa tattgacaat gaggatattt actggttgca atttaagggt ttctaataac
    62161 ttagctttgt cagaacattg aaatattatt ttaatatttt cattctggga gaataatgag
    62221 cttaaccctt ccattactat tttatgctca tcaaaaagac ctatcgaaat gctcatatca
    62281 aaacctgttt aaatgcaaat taaaacatta ttactgattt tttttctcgt ccgttcatca
    62341 ctattttcaa aggctttgca aattgtacat gtttaaaata gttagtttta tggattactt
    62401 tttgcttgtt aagtattttc cactgtataa attcagaaag agaagtgtcg tgcacagaaa
    62461 aatacccaat attcatagaa gttacgttat gaaaaaaatg agatcctaaa gatgaatcaa
    62521 gcgggaaatt actcaggctg atttctacaa tcacttttgc attagagatt tgtccccaga
    62581 ctacaggaat gccaagaaat tggtcttgag tgccccaacg tcccggaccg ataagaatat
    62641 actgccggtc gtttttttgc atcatattgt tcaaatgttc aatttctttg accatttcca
    62701 atgttttgag ccggtcaaat tttgccacat caacgtatat tacatcgcga atattgttaa
    62761 ttaccccatt gccaaggctt gactcggtat ataatatggt attttctttg tttatttcgt
    62821 caaaatcaaa attatggttg aactggctac ctacaagtgg tttgatttgc agcagataaa
    62881 aagagggcag accgttggcc gaaggggtta aatcaacagc atattctatt tcgaccggac
    62941 aaccttgagc ttctttgata gtgttgagta aaacgtcaat aagctgggca agagggatgt
    63001 agttgtattt taaaatattg gcaaaattta caatacgagg accgggagag ttgatgccgg
    63061 tttcaatacg atcattatca gggttataaa cagaagcaca atgtttcagt gttccatctt
    63121 tttcagctac actaatatca agcaatgcaa gaggtgccat ttctccatct tcaatgtaat
    63181 tgatgttttt cttggacata tctactgcta agaagtttac ctgtgtacta ttgagcaggt
    63241 cttttcgagt aaaaatttct attttcgggt actttggcga gaagcgaaat gactttcctc
    63301 cgccaacaac ataattgcca aggccaacag cagcaaccgc aaacccctct tcgggtttca
    63361 tgtgggcaac cggataatag ttgtatgatt gggctgtacc gctaatgtga gggtagtaat
    63421 aattgccgta ttgattcccg accagttcct gcaacacaac agccatacgt tcttcttcaa
    63481 ctttatgatg tatcgcctta aagtaggaac gtgaatggtc tgaataaata gatgcaaata
    63541 ccagttttat agcctttgta aggtggtcaa ggttaatttt tttattttta ctattattaa
    63601 gtacaataaa ggtgtcaaaa accccggcaa atggctgaga taatgaatct tcggagatac
    63661 ttgacgaacg tacagcaata ggtttgttta tccgcgaaat aaaatgttcc agcttgcgca
    63721 ttaagccatg tgagaggtgg gcatgaagaa aatgatgttt tatttcctca tacgaaatat
    63781 tggcattgaa aattttgtcg tacaaatggt tacgttccat gaaactttca aattcctcaa
    63841 ctccaataat agcagtaaca ggagttcgga tatttatttc tttgttaaat gccgaaaaat
    63901 cgaggttatg tatcaatgta ttgataaaag ccagtccccg tccttttcct cccaaagaac
    63961 cattggccaa caaataaata tttttttcgt caatgatgct tgtttcttca taggtaagta
    64021 ttttgccgcg tattttctct tctttgtagt cattgatggt tttaataaaa ttttgcctaa
    64081 aaacttcaat agactcaaac tctttaattc taaaagggtt tatggttttt gccaatttaa
    64141 tctcgcctcg cgacataagc caaagcgaaa actgattggc ttcactatgc aaaaacaggg
    64201 attcttcagg tatagtgtaa aggatatcgc gaaattcttt cagagtgtgg gcgacagcaa
    64261 gctgtcttcc ttctttatcg cggaatacaa aatctccaaa gccgagatga tatgtaataa
    64321 attgtttaat ctcagtaagc aggctttctg agtttttatt aatgaaactt accccgattt
    64381 tttcagctat tttttggttc tttacttccg aagattgcaa taaaattggc agatttttca
    64441 gattagcctt cacatattta ctaaacctga cccctgcatg tttatcaagt tttccatccc
    64501 tttcaaattc aacatcagag ataacacaaa gcagaaagtc tttatattta ttatatatat
    64561 acattgcatc ttcgtaattg cgggcatgaa gtattttcgg acgcgaacgc atcttactaa
    64621 tcttatcaat ctcgtttttc tctacttcgg gcaacaattg ctgaatttgg ccaaatacaa
    64681 cagaatacag tgtttgcagg tattttgaat aatactgtgc agaatcttcg ataaccagga
    64741 tgacacgtac caagcctacc ttagtgtcgt tttctacgtt ggcgctatct tctattgact
    64801 taacaatagc aaaaataatc tgagagttcc cgttccatac aaaaagttta tctacagatt
    64861 tcacggtagg cacaagttcc tcaaaatatt ttatattact tttttgattt agcaggagga
    64921 atataggcag gttgggccta acttgcttaa ttttctggct taaatctatg ggggcattag
    64981 aatcgatacc aaccataagg attacaaggt caaaactggt tgtatcaagg agctcaagag
    65041 cttcttcggc agatgtgaca cctgtaatac ggggcaacga gaacaaactg tactggtata
    65101 tttcgcccat gaactgctca aagaagctgt cctcattttc gaggataaaa gcatcgtaca
    65161 atgtagctac aaaaaggatg tccttaacct tgtacttcat catatcgaga taaatatact
    65221 tatccagaat ctgtttattg aaaaacagct gtaaaggttg cttattttta attaatgatt
    65281 ttcgatattc ttcagaattg tggcctgcaa tctctttaac ctgacttttg ttaaatattt
    65341 cgcggccttt atagttgtta aggtaaccac ttatcaattt agcaaggttg ataagcaact
    65401 gtcgttcttc tttcaaaaat gggccttcaa attcttcagg aaattctttg aggtaaaaaa
    65461 tctcaatcgt tccttttttg ttatcgaaag ttacaaaatg ctcgcgttgc ccccatattg
    65521 tttcactaaa atttttgctg gtataaatgt tgccttcaaa tttgatagca gcaacagtat
    65581 gttcggggta ttgccatgat tttgggataa tggcacaaat gtcccttaac gtatcatcaa
    65641 tactttcacc tttagcaata atggccgatg tctggttaat agcattgagc tctttgagcc
    65701 gttcgcggtt ttcgtattgt aattttctta atacatcttt tgtggctgat ccggcaataa
    65761 gagtagaaat gttgaccaac aggttgcgtt cttctttcaa aaacgggcct tcatctgctt
    65821 cggggaaagc ccgggtgtaa tatatttcga taaggccttt ggaattatca ggtgtttcaa
    65881 aaacctggtt tattttccat ttagtaacct tgaaattctt acttctgaaa actttatcct
    65941 tgtatgtgat acgggcaaca gtatgttcag gaaattgcca tgcttcgggc aagatgctgc
    66001 atattttctg caaagcatca tccatattat tgttctgttt taaaatttca gaagtttgat
    66061 ttattgcacg aagttctttc aaacgttcgg tattttgaag caaaagatgt tgtaatgctt
    66121 cttctgaagc cgtcccggaa atcaacaatg cgaggttgtc gagcagttgt ctttcttcgg
    66181 acaagaatgg cccttcgtct gcttctggaa attctttcag gtaaaatatc tctattttac
    66241 cttctttatt gtcgggggta ttaaagtatg cccgttggac ccactgcgaa tcctggaatt
    66301 ttttactttt aaactccata ttatcaaagg taatgcgggc tgcagtatga gccgggtatt
    66361 gccatgcttc gggcaaaaac aaacagatgt tttgcaacga ctcttcaagc gtatcgcttc
    66421 gttttaacac ttcagcggcc cggctaatcc ctttaagttc tttgctccgc tcatgggtag
    66481 cattggccaa cttggccaat tggtttttcg acaactcgcc ctctaaaagt gtggaaacat
    66541 cctttaaaaa agctgcattt tttgccacaa aagctttact gaacgagtcg gcatatgccc
    66601 caaaaaaatg aagttcaaca gctccaaggg taccatctgg caaattaaaa gtgtatttgg
    66661 tcaaacatgg tgaatcttga taattattac tgtcaataat gctagaatta taataaatcc
    66721 gggcacaaac ctctgaaggt tgaaggtaag ccctgggaat aaggttgcat acctttttaa
    66781 gcagctcatt gaccggatat tcctgctcaa aagaatggat taattgcagg tctattttag
    66841 aaatagacgg ctcttctgta acaccttcag cagagatatt ttttgttgcc ataatactcc
    66901 tttttcaata atttgctatt aaacttatgt ggttttctgt aaaaaatatc aaaacataag
    66961 ttttttttaa ttatgagggt taaaagtatg gcaaaaattt tcttttaagg tgatttttta
    67021 taatgttgaa atagtattgt ctgataaaat aagaaaaatt aatacctctt tatttaatcc
    67081 ttttcataaa aacaacctta tcatcacccg gggcataaaa gtctggcata atggcaactt
    67141 tatcataacc tatagctaaa tagaaagccc ttgttggttc gtaaataaca cgtcctgaag
    67201 tctcgaccca aacaatagtc cctccgagct tggcaatggc ttgttcggcc atagtcatta
    67261 gcttacggcc aataccccgg ttcatctggc ttttatgaac agcaatccag taaaggtcgt
    67321 agctcgattg ggtacaaggg tttaacccgt aacagcaata gccaagcatt gtgttatttt
    67381 cgtaagctac tataaagtga tagcccgaag cttctgcccc tttctcctga ttagcatctg
    67441 aaagctcttt tgccacatca atttcgtacg aataaaaaaa gcctgttgat tcgagaagtt
    67501 ccccgatagc ttggctatca ccagagacaa gttgtgtacg aaattctatc atgtttttta
    67561 gtttaacgtc agttcaacat aagaataata gctaatatta ggtatttaaa ggtcttaact
    67621 aaacttccct tacttttgcc ttgaagcaaa agtaacaaaa attcaaggcc gagaagccta
    67681 acgctcctgt tgtttgcata ttgcaacatt acaaggcttc gagggtgaaa gagttaa
//
LOCUS       DAOSPF010000011        64165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_492_length_64165_cov_7.594229, whole genome shotgun sequence.
ACCESSION   DAOSPF010000011 DAOSPF010000000
VERSION     DAOSPF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 64165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_64165_cov_7.594229"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="PK252_03720"
     CDS             complement(<1..1678)
                     /locus_tag="PK252_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT13850.1"
                     /translation="MTNQPLHCGIDIGSTTIKMVVLDEKKDKVLFSDYQRHHANISSA
                     LTDILKKAFCVVGNQAIAVCVTGSAGMGVSERYGLQFAQEVIAATDYISHKHREIQTL
                     IDIGGEDTKMVFLKAGSIPDIRMNGSCAGGTGAFIDQMATILAVDIAEMSRQALSAGK
                     IYPIASRCGVFSKTDVQNLIARNVSIADISASVFHAVALQTITSLSRGTEPLPKIFFC
                     GGPLAFIPALRQAFVNYLKLKDEDYIISENAALIPAFGCALTEKQDEKAVLLNSLIEK
                     IESINKQPHSISKGRLNKLFDNQAELEQWIALKQNHRLESITLEKLEGNECFIGIDSG
                     STTTKIVAIDDKERIVFQFYSRNKGNPLKTISEGLSEFLVLANSAKKDIHIVASSVTG
                     YGEDLVKAAYNIDHGIVETIAHYSAAQKFNPKVSFILDIGGQDMKAIFVENGAINRLE
                     INEACSSGCGSFIEGFAKSLNYTAANFATLACNANAPCDLGTRCTVFMNSKVKQFLRE
                     GASVADISAGLAYSVVKNCLYKVLKIKDVSELGDNIVVQGGTMRNHAVVRA"
     gene            1889..2368
                     /locus_tag="PK252_03725"
     CDS             1889..2368
                     /locus_tag="PK252_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13851.1"
                     /translation="MSPYSDRKEDFIIKPDYLSPVKVYSIVRPAIETGYVYNFTSDSG
                     LFYEVRFAPKVDNILGMVVNFTVDSDEFDSDYPVTNRGEVYNIIATVIEIIRIFHHFH
                     NFTTSYEFSGEFKENESKTEHNASIRSRLYLRYAPIVLSSNWKAQITGNKVLLKKVK"
     gene            2368..4062
                     /locus_tag="PK252_03730"
     CDS             2368..4062
                     /locus_tag="PK252_03730"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT13852.1"
                     /translation="MNPLLEVKNLCISFNNSGKAVDNLSFSLPVGQTLGIVGESGSGK
                     SVTGLAITRLLPKTTNISGQILAPHNWLDNAADDYIDLLQLPEKSMIKLRGKRISFIF
                     QEPMTALNPSMHCGKQIEEILKLHKIVEKSNLIERQLQLLKDVQLPNPEIVLKKYPHQ
                     LSGGQRQRLMIAMAMAANPDIIIADEPTTALDVTVQFEILELLKQMKQERNLTMIFIS
                     HDLNVISHIADNVLVMRHGNMMEQGTVRQVMNNPQHLYTKALLESKPSFHSRPVKLPT
                     VDDFIDEKPFYKEEESIELRKEKQNKIYNARPLIEISNLNVGFSLKSLFNKQQTLILR
                     GIDLKIWKGETLGLVGGSGSGKTTLGRTLMQLIEGYQGNIKYEGKKIKDFSKNEMLKF
                     RQKVQLVFQDPYSSLNPYQTIGQSITEPIIVHNLYKNNSEVRNRAIELMQMTRIQPEW
                     YSRYPHQLSGGQRQRVVIARALALNPEVLICDESVSALDVSVQAQVLNLLNELKEKIG
                     LTYIFISHDLAVVKYMSDRIVVLDKGLIVEDNEADELCQNPAHPYTQRLLSAAKME"
     gene            4183..5391
                     /locus_tag="PK252_03735"
     CDS             4183..5391
                     /locus_tag="PK252_03735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT13853.1"
                     /translation="MKTKLIIVSVIFIGLFSIFMLKGLSSYPEKPKMELNPAPVKSEL
                     EALYALYDSVMRAEMDSTGVVGASVAIVYNNKIDFLKCYGVKKVGETDSINPQTVFRL
                     ASVSKAVTGVLAGLLDNEGVISLNDKVIDYIPDFKLKSAVNTNNLRIKNILSHTSGLT
                     PHAFDNLVEQKMPFNKLMYNLSKAEISGKPGQLYSYQNVMYSLFDTIASVKTSKPYGE
                     LMSEKIFKPFNMYNASTSYESFKNNDNIAYPHKWTKNGYKPIPLNQGYYSTISAAGVN
                     ASISDMASFLLKLLRTDSLSLDSGVIRTIFTPQVVTPLNRHYFKEWGKVASRQYGIGW
                     RIIEYKGHQLAYHGGFIQGYRAEIALCREMNVGIVFLTNSPNKAATISIPTFLNMFFD
                     LKEKGVLAAK"
     gene            complement(5401..5955)
                     /locus_tag="PK252_03740"
     CDS             complement(5401..5955)
                     /locus_tag="PK252_03740"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT13854.1"
                     /translation="MENFQAVKNEKAYQLLNSGGLIIVCTVSSEGIYNMAPIAWHCPV
                     DYDKTTRLLFVSDLQHKTYANIIDTKKFVIALPSANQVEMVKELGSCSGHKVNKIEHL
                     HLSTFESPVLKIALPLDCIAYLECNLHSVVMQETVGIVLGDVINAFGQKDAYKGRLLS
                     ENLNAKTIHHLGSNNFITTADKLL"
     gene            6353..7243
                     /locus_tag="PK252_03745"
     CDS             6353..7243
                     /locus_tag="PK252_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13855.1"
                     /translation="MAHCIIKNILFITVLFLTFACRNESNSNVTLTEEDTLVNTEEVV
                     AKARSVFYRMYLPNEMYKVFEKAGAIYSPEILNPVENVNLYESSTKAALNLGVYGVDL
                     SYNKIFGQNQKTLLYFTVIHKLSQQLGIPDNIFSQALKKMEQNLSNRDSLTEYATDVF
                     SSTHKFLNENDRKSTAALIIAGGWIEALYIASQISDDDVENKEIIERIAFQKYSLRSL
                     MSMLANYQAEPSVNRYYLMFKALNNAYNEFEIYYFPDDVSIDTINKLINAKKVNIDLP
                     AGKIEKIKKIIAQIRQEITM"
     gene            complement(7246..7485)
                     /locus_tag="PK252_03750"
     CDS             complement(7246..7485)
                     /locus_tag="PK252_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13856.1"
                     /translation="MSSYTNIYAQKIYEILVPLIGDMMTKGTLKSQAAKLGINEESIA
                     PVHLPALAEELRRGLVAFLGSDAAKAVASKIMSIR"
     gene            complement(7488..8294)
                     /locus_tag="PK252_03755"
     CDS             complement(7488..8294)
                     /locus_tag="PK252_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13857.1"
                     /translation="MKKSKLLLIFIAVLTVLNVVYYLGQWGGPSVLQYVSDTLPVLTS
                     FIAVVCLYHTFKAFKEIDFARVSWVLIFIGIFFDFLAETTYAILEIGFKIDMNEYFPS
                     LADIFWCTAYIPLFAGLFMMFFGYKRSGFPLGKMKVYAIIAASSFVVFVAMAYFLLIP
                     ILSDNESDDITKLFYMYYPIADLLTVIPAIILFYITTLFGRAAISLPMRMLSIGFVCF
                     TMADMLYAYLGWLDIYGNGNLIDVLWHVGYLVIGLAGLYQKELVESVNAQ"
     gene            8483..9190
                     /locus_tag="PK252_03760"
     CDS             8483..9190
                     /locus_tag="PK252_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13858.1"
                     /translation="MKAIFFASFLAIVLVNTWAQEKDSSGLSLNLGADVVSRYIWRGQ
                     ANSSSPAIQPTLSLNYKNISFGSWASYTTSNNDLQEVDLFLTYKIGGFTVGLNDYFMS
                     SDSLDDLYFNWKNKETGHTLEPFITYSEIAGTPLSLTAAVFAYGDDLDENEDNYYSSY
                     LELSFAKTLGNIDYNIFCGGTFGKGYYADDMNIVNVGITLTKTIEITPSFSLPCNGSF
                     VINPSTENVFVVFGITF"
     gene            complement(9277..11121)
                     /gene="mutL"
                     /locus_tag="PK252_03765"
     CDS             complement(9277..11121)
                     /gene="mutL"
                     /locus_tag="PK252_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT13859.1"
                     /translation="MPDIIALLPDSVANQIAAGEVIQRPASVVKELVENAIDAGATQI
                     SVIIKDAGRTLIQVIDNGKGMSETDARMSFERHATSKIKRAEDLFDIHTMGFRGEALA
                     SIAAVAEVVLRTKRQEDELGTEINIAASEVLGQTPVSCATGTNFMVKNLFYNIPVRRK
                     FLKTDNYEFKLIVFELQKVALAFPHIQFELRNGNTEVLKLYTSPFRQRIIQLFGKNIN
                     QSLVDVNCETSIIKITGYITKPDVAKKSQPDQFFFVNNRYMRHPILHKAIIKAYEKIL
                     LPELQPSYFICFTIDPANIDVNIHPVKTEIKFFDEQAVWQILHATVKESLGKFSIVPS
                     IDFEIDRSVEIPVLSRATDVNLPQIDIDATFDPFKPEQKKSVSVRADFSSHSRSLPNN
                     WQDLYSGFENEPKTELRIPNFEPVENKGADDVQVSKQQQFLQVKNKYILTPSKSGLLL
                     IDQRRAHLRILFEQYLENIEKSANAVQKEIFPQVVELSPADHELIMEYSAAINQAGFD
                     IQDFGQNSVVVNGFPAALEQANPQKLVDDLLAELHHAPSQSGTSVKETIALALSKASA
                     IPYGRALGSEEMSDLIDKLFACKNHNYCPEGKTILTIINIEELENKLK"
     gene            11203..11928
                     /gene="recO"
                     /locus_tag="PK252_03770"
     CDS             11203..11928
                     /gene="recO"
                     /locus_tag="PK252_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT13860.1"
                     /translation="MLEKTRAITLHGLKYGDTGLVVHLYTEAHGRQSYFVKGAFSKKS
                     QIKSNLFFPLSLLELEVYHKPSSQLNKIKEAVLTPQLLTIHSNPYKNAIAIFISEVLY
                     KVLREEAANDSMFNFLFNSVCMLDLHHDSPSNFHLVFMCLLAKQLGFFPTNNYSSVNK
                     FFDLHEGFFVSQHPSHRQCMGAGMSEIFSELLGTNYENSAHLPIDRMMRASMLDAIAT
                     YFYLHMHGIGQFKSLQVLHDVFA"
     gene            11988..12734
                     /locus_tag="PK252_03775"
     CDS             11988..12734
                     /locus_tag="PK252_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOT13861.1"
                     /translation="MIARNAFLWIIAFVTISAFQQAKAQKTITFPSKDSLTITADYYE
                     ADPKAPFILLFHQAGSSRGEFTEIAPRFVKLGYNCLAVDLRSGNETKYVINQTALKAK
                     SQKKPTKYLNAALDMQAAIEYASNKSRKPVVVLGSSYSASLGLLLAKTNPQISAVIAF
                     SPGEYFEKENFTRDSIAGLDIPIFAASAKDEYKYLTALFGKVNRINYTLFCPSKGNGE
                     HGAKALWTESPENGEYWLALMLFINQIKDF"
     gene            12763..14172
                     /locus_tag="PK252_03780"
     CDS             12763..14172
                     /locus_tag="PK252_03780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOT13862.1"
                     /translation="MLFNTLHFLIFFVVVFVLFFTLPHKFRWVMLLAASCYFYMAFIP
                     IYILILAFTIVVDYFAGIMIEKSHDKRKRWFLIASLVANIGILAFFKYYNFLNNNLSL
                     LLGKFGYPDPIPALEIILPIGLSFHTFQAMSYTIEVYRGKQKAERHFGIYALYVMFYP
                     QLVAGPIERPQNLLHQFHNKHSFSIQNLSDGLRLIIYGLFLKMVIADNVSPYVNSMYA
                     NPQNYSSTDIITALCFYSIQIYCDFCGYSLIAIGSAKSMGFNLMNNFHTPYFSRTVSE
                     FWKRWHISLSTWFKDYLYIPLGGNRVALPRWMFNIFIVFAISGLWHGASWNFVIWGVL
                     WGLLNVGEVLYNKLFSRPEEKGDFKIWHSIHAAKMFIIATFLWVFFRSENMEKVKLLA
                     KSIKHNFSLNNSLNVPHLVWALLIVFLVFEIMFYNHRFDKWCEKRDPIIKWSVYIILI
                     SSIIMFAGIEDIPFIYFQF"
     gene            14264..15328
                     /locus_tag="PK252_03785"
     CDS             14264..15328
                     /locus_tag="PK252_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13863.1"
                     /translation="MIKIAKFIIPRVLVLAITLIAANFIYTKFFFEKDLQMHADMINL
                     TRGKDSARVIYMGESSNFTSHSDDKDPRSISGMLCDYFPGLNIQDISHGALHAYNYYI
                     FLKTIQNNSKLETIIITLNLRSFDANWRYSDLETFLQKQMVMIDPTYPPLFRRFLLGF
                     KGYDNKTVKERERQFLHEWETTVLKVPFPLQYNTVRKWDKGIATNGYIDVSGNRNEDS
                     TILACHYVKTYAFQIDTNKNPRIKDFDNIVKLAKQRGWHVIFNLMAENTQKADKLVGK
                     ELVYLIKQNRDLLVSRYSKMGAIVVDNLECVNDSNYIDQNWTTEHYKETGRKTIAKNL
                     SEALKTIYPQYYKSIVADNK"
     gene            15339..16325
                     /locus_tag="PK252_03790"
     CDS             15339..16325
                     /locus_tag="PK252_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOT13864.1"
                     /translation="MKVKFIILRFSSIGDIVLTTPVIRCLKEQVEGAEIHFVTKKKFA
                     SVVADNPYIDKIHLLDGKLKSLIEELRQEECDHIIDLHHNLRTYIVKKKLGLFAFSFD
                     KINIQKWLMVQFKINKLPQKHIVDRYLETCRLFDVTNDGKGLDFFINPKNEIEISQLP
                     SFLQNGYIALVIGGMHGTKQMPNAKLAELSNKLNLPVVLLGGPDDSQAAESIINESKN
                     ANIFNACGKYNLQQSASIVKQAKLVIAHDTGLMHIAAAFKKRIISIWGNTIPEFGMYP
                     YVSDENQAIFEVKGLKCRPCSKIGYKKCPKGHHNCMNLIDVNKIAEKANSWI"
     gene            16345..17292
                     /locus_tag="PK252_03795"
     CDS             16345..17292
                     /locus_tag="PK252_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT13865.1"
                     /translation="MITFFALILAIVLQFAGAIVAISNIRRTKYSISWILISLGFLVM
                     AIQRFIEFIPYVWRGWEKDVATINTWLGIITSILIAVGVIYIRKILDFLRKVEREKQE
                     SQKKILNAVIQAEEKERRRLAKELHDGMGPLLSAVKMAVSSLVKTETNSNNKLIIENT
                     DQMITEAIRSLKEISNNLSPHVLENFGLASAIKSFSSKISATRAIQITFFSNMFDIRL
                     NSNTEIIIYRVVCELINNTLKHANAQNIEIELKQHGKIITLLYTDDGCGFDTSTVLRN
                     ENKGMGLSNIINRIKSIKGAVNIESSHGKGLKVIIIVNT"
     gene            complement(17289..18140)
                     /locus_tag="PK252_03800"
     CDS             complement(17289..18140)
                     /locus_tag="PK252_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT13866.1"
                     /translation="MNEKTKAAGISVFSNTSLVIFKLITGFITGSVSILSEAIHSAMD
                     LLAAVIAFFAVRISDTPADKGHPYGHGKYENVSGIIEALLILLASGWIIYEAVVKILH
                     PGPIESIGLGSVVMFVSAVANFFVSRYLYKVAKKTDSIALEADALHLKTDVYTSLGVA
                     IGLLLIWATGWHFLDSVIAVFVATLIVKEAVHLLRAAYAPLLDASLSESEVEVIKDSI
                     KSHNLSFHALRTRKAGHYRFAELHLEVAPDISVGMAHEMCDMIEKDIMEKIKNIEVSI
                     HVEPLSS"
     gene            complement(18213..19817)
                     /locus_tag="PK252_03805"
     CDS             complement(18213..19817)
                     /locus_tag="PK252_03805"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT13867.1"
                     /translation="MSHKTPRFAYKFPKFTEADSLHGAISPRRACYDVTFYELFIRIQ
                     PQTKEVGGKVIMHCKALSDFEMLQVDLYPNLSFDSVLMNGKPLIFSRKHGAVFIYTPQ
                     KVAKGERLQLTMYYHGKPQVAKRPPWEGGMVWKNDKYGNTWAGVACEVAGSSLWWPSK
                     DQLPDEPDSILSHFIVPKGLYCVSNGVMIDSTAIGDEVTYTWKVHYPINHYNFTFYLG
                     NFAKFQRIYSSRFSNFDMDFYVLKDNYKIAQNHFLIVDSIISKFEQFYGPYPWPKDGY
                     KLVESPYEGMEHQSAIAYGNAYKIAPWIGTDHIILHETAHEWWGNSVSASDYADVWIH
                     EGFATYSEALFLEAAKGKDDYFKLLRFYAMLIKNKKPVIGPYAVNFWDYRDTDVYMKG
                     ALTLHTLRNSINNDSIFRDILKTFYLQNAYGIATTEAFMKLVNEKTGKDFTTFFNQYL
                     YSRVCPILLWEFSYDQNTGKNNFYYKWGNAVEGFSIPVVIKAGERTFIVRPTNKLQRS
                     ELPYSSTIIVNTKESYIAQKRTEVFK"
     gene            20164..20583
                     /locus_tag="PK252_03810"
     CDS             20164..20583
                     /locus_tag="PK252_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT13868.1"
                     /translation="MKKIIFLVSLVTIAFWGCKNNSSEYINKLEQKIKEDSLKNVAEM
                     QTLKSEMQARLDSMQQACGDCIGSFYVVTGSFREPQNADNYLAEMKKQGFKAQIIVAP
                     NGFHLVCIYCGQNLKDVLGALQSARNNVQPESWVYIK"
     gene            20645..21163
                     /gene="rimP"
                     /locus_tag="PK252_03815"
     CDS             20645..21163
                     /gene="rimP"
                     /locus_tag="PK252_03815"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOT13869.1"
                     /translation="MQSKYHSGNRGQKVPYFISYMNVEQIKNIVEEAIDKSKLFVVEV
                     NCSPSNKITVAVDAFHNITLDECVFISRAIESKLNRDEEDFELEVTSPGLSQPFKVHQ
                     QYLKNQGKEVEVLLKDGEKLTGILQNVSSDGIELALFAQKKKSKKPPVEEPEKEFYKF
                     EEIKWTKEHIKF"
     gene            21196..22431
                     /gene="nusA"
                     /locus_tag="PK252_03820"
     CDS             21196..22431
                     /gene="nusA"
                     /locus_tag="PK252_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT13870.1"
                     /translation="MENASLIETLLEFKEQKNIDRATLMRVLEDVFRAHLVKHYGNAD
                     NFDIIINIDKGDFEIWRNRHVVADDELTDPVSQISLTEAQKIDEDYEIGEDVTDEVKL
                     SSFERRSILSLRQNLNARISELEKSNIYAKYKDRIGEIITGEVYQVWKKEILVLDDEE
                     NELSLPKTEQIPGDHFRKGDTVRAVVYKVEMRNNSPQIILSRTASTFLERLFELEVPE
                     INDGLITIKKIVRMPGERAKVAVESYDERIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INFTNNIQLFIQRALSPAKINSIKIIEETKHAEVYLHPKEVSLAIGKGGLNIKLASKL
                     TGYEIDVYRESEQDEEDVNLDEFADEIDDWIIDELKAIGCDTAKSVLEIGAEDLVKRT
                     DLEEETVKELIRILKAEFE"
     gene            22600..25503
                     /gene="infB"
                     /locus_tag="PK252_03825"
     CDS             22600..25503
                     /gene="infB"
                     /locus_tag="PK252_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT13871.1"
                     /translation="MSDIKGVRLSKLAKELNVGINTITDFLKKKGHTIEHDPNAKISP
                     ELVTLLEKEFSSEISAKKESEKVDLKGIREKKETIVLQEKHQSIDKDNNQETETALSE
                     SKEKIELKVVGKINLDAPKVEKSAPKVNAEESKKEIVVNEKPKNIEKTAAKIEKPVDI
                     PLEVPNEIEAPKVVGRIDLDNMNLKTRPDKKTKEERIREEKEKQKEARANVTAKIAEP
                     QNKSEEVKAATEEIEEEIEETSESASQEDLIRMPIEKLNGPKVLGKIDLPSEKEKKLV
                     ISSSSIDSDKFKKKKRKRIKIEGQKVSVVPEQKAPAKPLEVKLKKDKKKFKKPFRPEV
                     SEEEVSKQIKETLARLTSKGKSKTSKYRREKREEIRERQQAELELQEIEKNILKVTEF
                     VSVNELATMMDVPATKLIATCMSLGLFVSINQRLDAETIVLLAEEHNYKVEFISAEIQ
                     EAIKEEEVDTPDKLLPRPPIVTVMGHVDHGKTKLLDFIRNTNVIAGEAGGITQHIGAY
                     SVQVADGKQITFLDTPGHEAFTAMRARGAKITDIAIIVIAADDGVMPQTREAINHAQA
                     AGVPIIFAINKIDKPTANPEKIKEELAAMNLLVEDWGGKYQSQEVSAKSGLNIDKLLE
                     KVLLEAEILELKANPNKNAKGTVVESTLDKGRGYVATILVQSGTLKQGDIVLAGTNYG
                     KVKAMYNERGKKIDVAGPSTPVLILGLNGAPQAGDEFHVLNDEKEVKEIAAKREQLQR
                     EQGLRTKKHITLDEIGRRIAIGNFKELNIIVKGDVDGSIEALSDSLIKLSTEEIQVNI
                     IHKAVGQISESDIMLAVASNAIVVGFQVRPSMSARRLAEKEQIEIRLYSIIYDAIEEI
                     KDAMEGMLSPEIKEEIVATLEIREVFKISKVGAVAGCICKEGKIQRNTKVRVIRDGIV
                     VYTGELASLKRFKDDVKEVLPGQDCGLGISNFNDIKVGDIIEGYKETEVKKKL"
     gene            25673..26848
                     /locus_tag="PK252_03830"
     CDS             25673..26848
                     /locus_tag="PK252_03830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT13872.1"
                     /translation="MPINVFVPKFRTDEILEEIKECLEKGWTGLGFKTVEFENHWKQY
                     TGMPNAHFLSSNTAGLHLALNIFKEQNKWADGDEVISTPLTFISTNHAIVYERLTPVF
                     ADVDEYLCLDPKSVLKAITPKTRALIFVGIGGNSGQYEEIVKICKEHNIKLILDAAHM
                     AGTKLNGKHIGSDADVVVYSFQAVKNLPSADSGMICFADQQLDNLARKKSWLGISKDT
                     YQRFNKNEGSYKWKYDVESIGFKYHGNSIMASMGLVQLKYLDEDNTYRRKLASLYDKK
                     LENIKNIQIIKVAPNCESSRHLYQICVPNRDEVMQFFYDNGIYPGVHYIDNTEYTVYN
                     YAYGKCPNASLKSKMLLSLPLHLNLTENDLNLIVSTLEKALKNCVKPNESLISDFSK"
     gene            26967..27848
                     /locus_tag="PK252_03835"
     CDS             26967..27848
                     /locus_tag="PK252_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT13873.1"
                     /translation="MKKVAKSIHKKLNNLNFFFTSLFFTNIFIQKVYLPRWEIKIFKY
                     CGKNIIYDFDDAIYVNEQSQAISKNKRKRLDYVLKQADWITVENETNANYAKICGNNN
                     SIIITGPIECNRYFPQENTNSGNEFVIGWIGSPSTTKYLEIVKRPLQILSQTHPNFKL
                     MLIGANDIAIEGVNTENYPWTLDSEVELLRKFDIGIMPLYNDIWCAGKGGYKLLQYMA
                     LGIPSIASPVGVNDDILNMGDIGFKAITEEDWIKSFEKLINNKEIRLQMGKKARETAL
                     TYFSYEKYVHDLIHCFR"
     gene            28321..29265
                     /locus_tag="PK252_03840"
     CDS             28321..29265
                     /locus_tag="PK252_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HOT13874.1"
                     /translation="MNTQITQLFGIQYPIVQGGMVWCSGWELASAVSNAGGLGLIGSG
                     SMQPEILREHIQKTKAATSKPFGVNVPLLYSQIEKTMDVIMEEGVKIVFTSAGNPATW
                     AQKLKDKGIKVVHVVSNVKFAQKCQDTGVDAIVAEGVEAGGHNGREETTTLVLIPLIK
                     KHVNIPVIAAGGIATGQAMAAAFALGAEGVQMGTRFAASIECSAHEAFKQQIVALSDG
                     GTYLALRKKAPVRLIRNAYANRIEEAENSGQSLEQVLQLVGKGRSKLGMFEGELNEGE
                     LEIGQAAALIDAVQPCDVIMQNIISEYNQAVKGLKILD"
     gene            29323..30876
                     /locus_tag="PK252_03845"
     CDS             29323..30876
                     /locus_tag="PK252_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT13875.1"
                     /translation="MKNIIATIIILFTSLSGSLMAKDPIPSPEKIRITNGSILGNNED
                     ANYYTIRWNYNNLNLVDYYVIYSEDKVSPNGWKEEGKQPINTKKEFPLNSFNGEQISQ
                     YTVEAVAKDQANYDDSKTNMTNSYTAQNIVLGTAYNACNNELTFTWNSNPLVTPRSQV
                     IRYTIDGVYDEKKYFSVTNTENKYILATNKLTLKKGVQYKFQLEILYKDVEDYTYSNI
                     ISFTIPETAKTFPDYINIDKLDASSGKVVLNINIDPKNELTDLWVFRSLSTEQTKSDI
                     IKIKLGSAEYPSLNNVSITDDSELELKTSQYKYTVSLLSCENVVKSSSIFTLPLEDPQ
                     EREGSLDLWWKPIQPSTQLDHYEITRYRNNGSSTLETPANTAINQRDYLNKIKDKQPG
                     EFICYTITAYMNVNFGTSVSNKVCYQIDEMVTMPKYIVIGENNKAISDFTFPSNSYTP
                     EKFTMRIYDRLGTKLFETTDPQKGWNGCYNNGNGSPVPAGAYIYSIDFTGNDGKIKHQ
                     QNSFIVVHK"
     gene            30894..33185
                     /locus_tag="PK252_03850"
     CDS             30894..33185
                     /locus_tag="PK252_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOT13876.1"
                     /translation="MSKLTDEEIKEINELYRLLITNSPRCKEDEEKALVDKAFRIAFD
                     AHKDMRRRSGEPYIIHPLLVARIVSEDIGLGVKSIISALLHDVVEDTEITLEEIEHTF
                     GPKIAYIIDGLTKTKEIEKNSIIPDLIGEVSEEVRSKQLDNFKKMLLTLSDDVRVIFI
                     KLADRLHNMRTLSSMPRDKQLKIASETIYLYAPLAHRLGLYSIKTELEDLSLRYRNPE
                     IYNDLQQKREEAVKQQDEYIKNFIDPISKKLKDNHIEFEISSRFKSVYSIWNKIQNKK
                     VSFDQIYDIFAIRIIFAPSADISEKTQCWNIYSIISDLYMPMPERLRDHITKPKSNGY
                     EALHITVMGPQGRWIEVQIRTKRMDEIAERGLASHFKYKNDNQQESELDKWLRDIREM
                     LKNPDSNALEFIEEFKLNLFSSEIQVFDRSGKLVTLPKGSTVLDFAYYIHSKNGNTAM
                     GAKVNHKLVPINHVLSSGDHVEIINSDKNRVQREWLDFIVTARAKSAIKNVMKAENRN
                     RLEKGKKMLESAFKQWGQSLNSKSLRTILEEFRMSNKEELYSKIGSEIILLDDIHKIF
                     KKESSNKWMKYWKLTIGRATGISLGSSDKPHPPVKKSNKFSKDNPFILTENIEDNTRS
                     YIIAPCCTPLPGEEVIGFLNVRDEVIIHKSKCPNAVKLLASQSNKIVPTRWTTHKILS
                     FPGKIHIKGIDRLGIYNDIISTISKDLNINIRSINMSSNNGIFEGEIELYVHSINDIN
                     KLTVTINKIKGVDFVKRVENIEQ"
     gene            33191..33997
                     /locus_tag="PK252_03855"
     CDS             33191..33997
                     /locus_tag="PK252_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT13877.1"
                     /translation="MGLHFRKLGQGQPLIILHGLYGSSDNWYSIAKVFSAYFTVYTID
                     LRNHGHSAHYPTHTYNDMAADIVNFCQEHNIEKATFAGHSMGGKTAMLLALEHPQLIE
                     KLIVVDIAPKAYSLLLEPSEHVLQHINIMQAFANVDLTGLTKREEVDMLLAEYIADPP
                     TRQFLMKNLVRKESGFQWQLNTKVLRNSLPEIINGPDFNKILFDKSQITYPVLFVKGE
                     KSGYIKDEDMITIRKIFPKAELSVIFNAGHWLHAEQPQSFINTLKYFLCL"
     gene            33994..34365
                     /locus_tag="PK252_03860"
     CDS             33994..34365
                     /locus_tag="PK252_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT13878.1"
                     /translation="MKIFDKLADKITLRKANALELIPVKLVNAKDENIMPITLLVPRF
                     NNRFLTGIIRRPEHFTVRLDETGSKVWLLIDGKRNVFEITQHLCAPNEPSDEWFRRVA
                     EFVTSLYKKDLVKLEVKKQFY"
     gene            complement(34486..35652)
                     /gene="hflX"
                     /locus_tag="PK252_03865"
     CDS             complement(34486..35652)
                     /gene="hflX"
                     /locus_tag="PK252_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT13879.1"
                     /translation="MINLAPPAETAVLVGLISSQQDEATAREYIDELEFLADTAGAVV
                     VKKFFQRLDVANPRTFVGEGKLLEIAEFIKENEITLVIFDDELTPSQLKNIEKKTNCK
                     VLDRTNLILDIFAKRARTSHAKTQVELAQYQYLLPRLVGMWTHLERQRGGIGLRGPGE
                     TEIETDRRVIRDRIARLKEQLKKIDTQMATQRKNRGQMVRVCLVGYTNVGKSTLMNLL
                     SKSDVFAENKLFATLDTTVRKVVVGNLPFLLSDTVGFIRKLPHHLVESFKSTLDEVRE
                     ADILLHVVDISHPSYEDQLAVVNQTLKELGAANKLTYVVFNKIDAYMPDNPLCVEELN
                     NSYIASVNAPCIFISAKNKTNIENLRKTLYDKVKEIHITRYPYDNFLYPDGEFV"
     gene            35846..36427
                     /locus_tag="PK252_03870"
     CDS             35846..36427
                     /locus_tag="PK252_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT13880.1"
                     /translation="MGVLQSLVLKNRSYRRFKEDENISSDFLKELVSLASITPSGRNA
                     QPLKYVLINTPETNLKVFNTLSWAAYLKGKGAPNEGERPSAYIVILLDKHISENPFCD
                     HGIVAQTMLLGAVEKGYGGCMIMNIKKEELSNILNLPEHFEILMVIALGKPIEQVVLE
                     TMPQDGNFQYWRDEQQVHHVPKRSVNELILKIQ"
     gene            36440..39751
                     /locus_tag="PK252_03875"
     CDS             36440..39751
                     /locus_tag="PK252_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT13881.1"
                     /translation="MKINQLVLRGIALLLLIIAATSLINSQTGLPYISNFTISEEDLT
                     ENYSLVQDSQGIIFTANRKGILSFDGEDWQIIKTPDMPTVLAISSATNTIYVGCRNTV
                     GYLSKNSLGKYEFNSLSDHKTGFVSQIIVHNNNVYFLSDSLLLYVTDNSLPSKVVVAN
                     NQANKFMCMIPFGSQLFIDIAGKGLFSLKNNKADEKVKNFTLSEQVVFTIPLEKSTII
                     GASDNKLYFFDGKNVSAFRSQADQILLQGTIIGGKKVTQSVIAIATAYAGCIFIDIKT
                     GKTIYTINTQNGLPDDAIYAIEVDQNNGIWLTHGYGLSRIDINLPVRNFSHYKGLDGK
                     LHAIAMFKSKLYIGSTTGVLVLNEKKIFDVSTRQQTIKPIQPAASGWASDQPQVSAEK
                     RGFWSKLFTKKINPENEKPTNKQPFARSEKSIKIFKQIAERYEYEKVQGINSRCKQLI
                     VYSNHLLAYTTNGLYEISGNSATQILSGNDINYLIPVNDDKALYVCTEKGIKILKDEN
                     GKLISSDFEIMQNQPVYSLAKDMFDNYWFGLENKVIKIKVKNGKIKEKKAYNFNSDFR
                     ERIIVRISNKKPVFFLSDGVYSIFNDSIQPNLSLSKYINKDSKVYFPAGNHTYINSNN
                     QWIKIEENREVDSTSYQYLGLFSNINQICSDKDSNFWVIDESTNVYMLNAKEIEKYKT
                     DFKAFVKKFFSNLSGQDFALNNVTLERKDNYLKIKIGAPYYIKKGTNQYQIYIEKLMP
                     GWSAWSNNSEFDIPLSPGKWQIQVRAKNILGEISEPRTISFNIKKPFYNSWWFYMLCI
                     AALVGVIYLIIKFRERQLQHEKEVLEEKVRERTREIEKQKEEIELQRNKIIKINNEII
                     DSIRYAKRIQAAALPSDYIEPSYKLKTFVYYKPKDIVSGDFYWIKQKNDIAMIAAADC
                     TGHGVPGGFLSMLGISFLNELAAIEQKLTAAEYLNMLRVKVKTTLSSKMEDIESKDGM
                     DIAFCIVDVKNKKLQYAGANSPIYLVRQNELIQYDADKMPIGSFIAEKDSFTNHDILL
                     QDGDRVYLFSDGYRDQMGGPMQKRLKTPGFKQLILQASQMPIVEQHKHFESFFEKWKG
                     DNEQMDDILVMGFGI"
     gene            complement(40009..42450)
                     /locus_tag="PK252_03880"
     CDS             complement(40009..42450)
                     /locus_tag="PK252_03880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT13882.1"
                     /translation="MKKLLSILTAMAMPMLAMASEADLKVPEAIKSEGILYAGFVVCA
                     LGLLFGLYQFMKVKKLPAHQAMLDVSEVIYKTCSTYLKQQGKFLAILFIFIGAAVAGY
                     FGFLAKDHEGHSLFGFGGVLLILAWTIIGILGSYSVAAFGIRMNTLANARMAFASLEK
                     KPLKLLNIPLTAGMSIGVMLICIELIMMLIILLLMPGHLAGACFIGFAIGESLGASAL
                     RIAGGIFTKIADIGSDLMKVVFKIGEDDPRNPGVIADCTGDNAGDSVGPTADGFETYG
                     VTGVALISFILLAVGGSALGMDEIVTSGLMVKLLTWIFVMRILMIITSIVSYYINGML
                     SNAKYGNVDDLDFEQPLTNLVWITSILSIIVTFAASYVMIPDLNGNTNMWWILSTIIS
                     CGTIGGALIPEFTKIFTSPKSAHVQEVVESGKEGGASLTILSGFIAGNFSAFWKGVVF
                     FVLMFIAYFMSTKGLSEIMVYPSIFAFGLVAFGLLGMGPVTIAVDSYGPVTDNAQSVY
                     ELSLIEEVPNVGAEIEKDFGFKPDFEKSKHYLEANDGAGNTFKATAKPVLIGTAVVGA
                     TTMIFSLILMIQKTLGVQPETILNLLNPYTILGFILGGAVIYWFTGASTQAVSTGAFR
                     AVAYIKEHINLDPNAPKKADAKSSSEVVKICTQYAQKGMFNIFISVFFFALAIACLSS
                     PESVGGVAAVSLFVSYLIAIAVFGLFQAVFMANAGGAWDNAKKVVEVDLKAKGTELHA
                     ATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAMEIAISENFRAAAPYVGLIFIAVA
                     LVFVWRSFYKMRIEK"
     gene            42808..43428
                     /locus_tag="PK252_03885"
     CDS             42808..43428
                     /locus_tag="PK252_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT13883.1"
                     /translation="MNEELKQIIEKVQALYFKYGIKSVTMDDVARELGMSKKTLYQHV
                     SDKNDLVQKVVNYVMERDHDEFDEIFENHKNAIEELIEMTLYINKVMKNHNPSTIYDL
                     KKYYPNLYKVMMEKRRDKMFQHTINNLKKGKEQGIYRTEIKEDIIAKYTTILTFQTFE
                     NDTLDMNEFTSPDFFNEVFSYHIRGIANAKGLEILEHNLNKITENK"
     gene            43447..44868
                     /locus_tag="PK252_03890"
     CDS             43447..44868
                     /locus_tag="PK252_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT13884.1"
                     /translation="MKRACLDAIKNSNESRLVGTSSIRHLKNKLVRIKEKKLLLSVLF
                     ICLTLQTFASGGADSSMQFSLEQAKQYALENSPLVKSAALDLEAAKKKIWETTAIGLP
                     QASAKYSYSYILTLPEIYKQFAQMGGGGEANLDDMKSSSTLDITVSQLLFSGAYLVGL
                     QTSRVYKGLSELSLTKSKNDLNESVTNAYLLVLIAKENKKILDTMYNNMNALLETIKA
                     MNSKGFVEETDVDQFQITVNTVKNNLELVTNQVLIAERMLKYQLGYPFESAITLTDSI
                     NGFVNNININTILAEPFQVENNVDYKLVETQTKLMKLNLKLNKSEFLPDIAAFYQHDK
                     NFNDKAVSFTPPDLVGLTINIPIFSSGQRLAKVSQARISYEKAKISQDQTAKGLKIQF
                     EDNKAKYINALGNYESSKSNYALAEKIYNRSITKYKAGMISSSDLTQVQNQYLQAQTN
                     YFSAILDLTSAKSKIENLISTVK"
     gene            44889..45986
                     /locus_tag="PK252_03895"
     CDS             44889..45986
                     /locus_tag="PK252_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT13885.1"
                     /translation="MKTKLLSLAFILVLVSCGKTDKKAEIAKLRKQQAELAEQIKRLE
                     AEVAGDSTNVAKGTRVGITDVAYQDFIHCIEIQGKVDGDESVDVFAEGTGGIIKQILV
                     KEGQYVTKGQVLATIDDKIYQVQLKSNEANLEYVNSLYEKQKALWDQKIGSEVQYLDI
                     KNKKESLESAVETIKEQINMCKIKAPINGTVEGITIKIGQLVGPAIPAFRVVNLSTLK
                     ITAEVAEGYSSKVRKGDKVSIYLPDMKKEFNGNVDFDSKYISPVNRTFTVNVRIPSND
                     KDIKANMVAVVRITDYHNPKAIVVPLNVVQSDLNGQYVFVAQNKTAKKVHITTGEIYA
                     GNIEIKSGLNIGDKVITVGYQELEDGQAINF"
     gene            46069..49509
                     /locus_tag="PK252_03900"
     CDS             46069..49509
                     /locus_tag="PK252_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT13886.1"
                     /translation="MEKLKFKEFKPTSWSIDNKTSIYVLTFIIAVFGLINYNTIPKEQ
                     FPEIVIPQIIVSTVYPGTSPADIENLITRPLEKNMKSINGVKKITSKSVQDYSLIIAE
                     FNTDVKVADAKQKVKDAVDKTKKDLPSNLLEDPFVQEIDLSDIPIMYLNISGNYDLAK
                     LKKFAEDLQDRIESFPEITRVDIVGALDREIQINVDMYKMQAAKITFYQITQAIGNEN
                     MTIPGGTINMQNMTRSIRVVGEYKNIDQIKNTVLISNSGAIVYLKDIAEIKDGFKDQE
                     SYSRFNGKNVITLNVIKKSGKNLLDASDKIEAAIAEFKENKFPSDLEVSITGNQAKFT
                     RSILTELNNTIIIGFILVTIVLMFFMGVTNAIFVGLSVPLSMALAYIFMPWIDFTMNM
                     LVMFSFIFALGIVVDDAIVVIENTHRIFKKENGRMTIKEAAKNAAGEVFLPVLSGTLT
                     TLAPFFPLAFWPGVVGSFMFYIPVTIIITLFASLLVAYIINPVFAVDFMKHDEDHTVK
                     LHQKKAYKNGAIIIAIAAFFYIVGGFKYGSTMFGIGTFLVLVAILYVGHNTFWYKIIH
                     KFQNRVIPGLMSRYDRILRWAIEGKRPYKLLWGTFILLIVSFILTAIAGPKVVFFPEE
                     TPKTIMVYAKLPVGTRVEYTDSIAKEIEKRVNTVLGATNPDVESIVTNVAFGASDDMF
                     DGGIVTSNKAKIAINFVEYNKRTVNYNTMVYMNPLREAMKDIAGAEITVDKNQDGPPT
                     GKPINIEISAEDLDELITTTDRFIRFLDSINIPGIEKYKTDFQKTKPEIIVDIDRLRA
                     NHEGISSAQVGSAINTAVLGTETSKFRDGEDQYPIQLRFSKAQREDVDKLINMEITYR
                     DMNSGMLRSIPLSSLAIVKYQNSVGGINRTDLKRVITVTSNVLDGYNANEIVAKIRKA
                     IPEFQKSDKIDIKLTGEQEDQAETMAFLSKAMILALFLILFILITQFNSFGKTAIILS
                     EVIFSIIGVLLGFTICNMTFSIIMTGMGVVALAGIVVRNGILLVEFTDVLKERGLRTR
                     EAIIQAGKTRITPVILTATAAILGLIPLAIGFNINFATLFTHLNPHIHFGGDNSMFFG
                     PLAWTIIFGLIFATFLTLVLIPVMYFITYSGKVKLARARHRSKIETMRELEEDELNEY
                     "
     gene            49721..50893
                     /locus_tag="PK252_03905"
     CDS             49721..50893
                     /locus_tag="PK252_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13887.1"
                     /translation="MQYQVIEVNDKKTAREFLLFQVKLYKNEKYYIRPLDTDIEKVFD
                     PKQNKLFRQGECQRWILKDEKGETVGRVAAFVDRKSAAKNDQPTGGMGFFDCINDKTA
                     AFALFDTCKKWLETRGMEAMDGPVNFGERDRWWGCLIEGFTYPNYCNNFNFPYYRELF
                     EAYGFQDYFRQITYHSPIDDSNMSPLIREKAKRLSSNPDYHFKHIEKGNNDKYALDFM
                     TIYNKAWAKFPGVKPLAEGHVKAMFKSLKPVMDEKLLWFGYYKNEPIACFIMIPEINE
                     IVRRLNGRFDFIGKLKFLYYKWRKVCFKSFGFVFGVVPEQQRKGVEGALITAYANMAC
                     KPGFPYSELELNWIGDFNPAMMHLVEEIGCKPVKIHVTYRYLFDRTKEFKRCTKVS"
     gene            50949..51086
                     /locus_tag="PK252_03910"
     CDS             50949..51086
                     /locus_tag="PK252_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13888.1"
                     /translation="MIFIYRTLTKKNGSVKSIIDRKVHEEKIKVAKFYVQIINTLCAL
                     R"
     gene            complement(51166..52155)
                     /gene="trpS"
                     /locus_tag="PK252_03915"
     CDS             complement(51166..52155)
                     /gene="trpS"
                     /locus_tag="PK252_03915"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT13889.1"
                     /translation="MEIVLSGIRSTGNLHLGNYYGAVRNFVKMQHNYNCYFFIADLHS
                     LTTHPKPGDWKANVKQILCEYLAAGIDPEVATIYVQSDIPEIAELYLILNMLAYKGEL
                     ERTTSFKDKVRQNPDNVNAGLLTYPVLMAADILIHRANKVPVGKDQEQHLEMTRNFAS
                     RFNNMYGNDFFPLPTAFNFGSDLIKIPGLDGTGKMGKSDGNGIYLADDAKTVEKKVMR
                     AVTDSGPTHKGQKMPEPIRNIFTILEVVSEPDVVKYFKEQYHECTIRYGDLKKQLAKD
                     INLSIDPIRERIKQYLSDDAYLRKVTKMGQEKARESAIKTLKGVREIIGITGF"
     gene            52264..52617
                     /gene="rbfA"
                     /locus_tag="PK252_03920"
     CDS             52264..52617
                     /gene="rbfA"
                     /locus_tag="PK252_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT13890.1"
                     /translation="MTSSEQTPRRLKVARLVQKEIGNHLQREAFTLCKGRMVSVTNVR
                     MTPDLAIARVYLSIFPSDNADEVLNNIKLHIKSIRHFLGNQVHQQLRIVPDLEFFIDD
                     SLDYVAKIDNLLKKS"
     gene            52643..53452
                     /locus_tag="PK252_03925"
     CDS             52643..53452
                     /locus_tag="PK252_03925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT13891.1"
                     /translation="MKYEPIKASLGKVFNNIPVLRIIFYKMLDLLLLRAWHIKKELRK
                     IKKQTGPNASVLDAGAGFGQYTYYMSTFSKGWSITGVDIIDAQIEDCNRFFAKINKSQ
                     QVKFEKADLTAFLRPESFDLITSVDVMEHIEDDRAVFSNFYKSLKKGGCVLISTPSDK
                     GGSDAHDNDDHSFIAEHVRNGYNIDEIAEKLTTAGFSKVECLYQYGTPGKISWTLSMK
                     FPIIMLNKTKLLFILLPFYYIVTFPFCFILNYFDLQMKHAEGTGLIVKAWK"
     gene            53456..54670
                     /locus_tag="PK252_03930"
     CDS             53456..54670
                     /locus_tag="PK252_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT13892.1"
                     /translation="MSFPFFISKRYFVSKKSQSAINIISWISLGGVAVGTMALVVVLS
                     VFNGFDDVIQSLFNSFDPDIKITLKEGKVFDLKADNIAKIEHLQEVDKVTYVLEESAL
                     LQYGDKQYIAQMKGVSANYPESNPIKSKVSHGEFLLSKNNVPFALIGAGVAYYLGVDP
                     NFVKPLNIYIPRRDADINDAENAFTHQLISISGEFSIEQDIDAKYFIVPYDFASKALE
                     YSNKVSALEIKLKDYSLVEKAQEKISQIVGTEFHVKNRYQQQEMFYKIMKSEKWAIFF
                     ILTFILIVASLNIIGSLTMLIIDKKRDIQTFGYLGANWKDIRRIFLFNSWINVIIGAV
                     IGLFIGLAVCLAQIYFGFIKLQGSGSFVIENYPVKIVATDILLVFFTVIVIGYITSLI
                     PVRAISRKYFED"
     gene            complement(54673..55536)
                     /locus_tag="PK252_03935"
     CDS             complement(54673..55536)
                     /locus_tag="PK252_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT13893.1"
                     /translation="MKKGYILLISAEFFFAAATVFAKFATTNTDIPALEATFMRFFLG
                     FIITWMGMRHEQLSFKPNNVKLVGARVFFNTIAVILFFLSVKYTTITNANMLNMTYPV
                     FMLFLAPWLLKEKVAGKQYLFLLIAMIGIALVVHPDFHAINIGDLYGLLSGATGAVAI
                     ASLRIARKYDSTHIIIFYLMGIGALINAILLIPSFVMPTWWQFLWLSVSGLLGYVGQV
                     FLTAGYRYVDATKGSMLSSSRIIFAFFLGVLIFSEPFAWPIITGIVLIVFAIIGVTRP
                     SAIFIKQLSRK"
     gene            55936..58791
                     /gene="galA"
                     /locus_tag="PK252_03940"
     CDS             55936..58791
                     /gene="galA"
                     /locus_tag="PK252_03940"
                     /inference="COORDINATES: protein motif:HMM:NF041462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HOT13894.1"
                     /translation="MKKLTIILSVVVSISFCYALRAQDIQVRERINFDKGWLFALGHS
                     FDASKDFDNGTGYFSYFAKAGYGDGAASANFDDRAWRVLDLPHDWCVELPFSEKGGHS
                     HGYKAIGRNFPENSVGWYRKKFSIPESDLGKRISIEFDGVHRDAIVWVNGFYLGTEHN
                     GYASYAYDITDYLNYGGENVVAVRVDATMEEGWYYEGAGIYRHTWLTKTNPLHVARYG
                     TFITTELSNNNTVAKAVVRTTIVNEAREEQTFSLEDMIVDAENKAITSGSKTNLKLKP
                     GESKCFYSTYTLKNPNLWTLEKPYLHKLLTIVRTNNKVVDTYETNFGVRTVRFDANEG
                     FFLNGKHVKIVGTNCHQDHAGVGTAIPDGLQEYRIKKLKDMGNNGIRTSHNPPTPEML
                     DACDRLGMLVLDENRLMGCNDEHFKYLKNMMLRDRNHPSVVLWSLGNEEWAIEGNIKG
                     ARIAATMQAYAQQLDSSRAFTAALSGGWDTGIGTVTQVMGYNYIVQGNIDEHHKKFPW
                     QAGIGTEESNTVGTRGIYETNDNLCHLAPTNRMPENVGTESGWQFYAARPFLAGLFYW
                     TGFDYRGEPTPYGWPAIASQYGIVDLCGYPKDIFYYLKAWWGKDPVLHIWPHWNWKGK
                     EGQPIKVTVYSNCDEVELFLNNKSLGKQTMPVNGHLEWAVNYEPGVLSAKGYKGGKET
                     LQSKVETTDVEDGIQLTTDKSAISSDPNDISVITVNVTDKNGLVVPTAMNEVAFTIEG
                     EGRIIGVGNGNPSSHEKDKYTETIKLSKNVDLKEFSVVSLENCPQVDPKVDASGWKAA
                     FTTQSKDWKVYTDSLIVVRGTFEIPGFTNETQINLFTKSIVENQSVYVNGRLIKANIK
                     RDAEGQSFVLDHSILKPGKNVFAVTGQRFRKKHQWDEPNTDPGLVQVIIPAEQWKRKV
                     FNGYAQVIVQPTGKTGNIKLSASSAGLKPASLIINVK"
     gene            58928..60181
                     /locus_tag="PK252_03945"
     CDS             58928..60181
                     /locus_tag="PK252_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT13895.1"
                     /translation="MLDNFYQPQSLVIVLLALLLTARVLGEIAERFKIPAIIGEVMAG
                     IILGPAILNWITPNESISVLSDLGVFFLVVLAGLEIVPHDIRTSLKGRNIFYIVFASL
                     IPFISGALIGYLFKMPLSTCLFIGLCFTVTALPISARILLEMNKLNTPTGQRIISIAV
                     IADVLSLLLLGVVIEVNNLQNINQFSQAVGFSLLKLVLLLFIFVLLYRIYQYAKLHFD
                     VIRQKVDLLMLLLKGNESIFALVIVFILLFAGLTQFIGLHFIVGAFFASMLLNRQILG
                     TRNYIRVRRNLYTVTMGLLAPVFFASIGLSFKVFDMSYFLFFIVLVAGVTISKTLSGL
                     LGARLAGHLQIEGLTTGLGISMSGLMGLVIANVAYTQHFINNAMYSVLVSMGLFTTLV
                     IPVLLKKTFVILDKKSALCLDSASN"
     gene            60157..61572
                     /locus_tag="PK252_03950"
     CDS             60157..61572
                     /locus_tag="PK252_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT13896.1"
                     /translation="MFGLSIELTLILFFCLIAACAFEFINGFHDTANAVANVIYTKTL
                     KPAYAVMWSGLFNFLGVMLGGISVAMGIMKLLPTSVLVDQNVYHSLALILALIFTAII
                     WNLGTWYIGLPCSSSHTLIGSIFGVGIAYMLLPDVGAVALNWTKVKDAGLSLLISPVI
                     GFGLALGLYFLLKKVTKKNQVLFEHDEKRKHPPVWVRALLIFTCTSVSFAHGSNDGQK
                     GVGLVMIILIAFLPARFILNPHLETASISSKIEVIQAVLVSADTAKLSDDNKIKLTGI
                     LAKTNKVEAISLKTKNGHSPTINDKYEIRRDLGFIQTETKDLMKITVEQPQVYSNKQV
                     AVVSTELEALKKYTEYAPYWVILMISVSLGLGTMIGWKRIVVTIGEKIGKTPLTYAQG
                     VSADLVTASTIAMASNFGLPVSTTHVLSSGVAGTMVARNGLKNLRKKTIRNILTAWLI
                     TLPVTIVLSAGLFLLLRWIFS"
     gene            61645..63621
                     /locus_tag="PK252_03955"
     CDS             61645..63621
                     /locus_tag="PK252_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOT13897.1"
                     /translation="MKPHESSHIHKVSFAGLLVTLGIVFGDIGTSPLYVMQEIAIATG
                     KIDSSYILGVLSCIIWTLTLQTTIKYVLFTLRADNKGEGGIFSLFALIRRRVPLAYIF
                     AIIGGSTLLSEGIITPAITVTSAVEGLRLINTDIPVLPVVIAIITALFLVQQFGTKLI
                     GSSFGPIMFVWFGMLGILGFSHILDMPMVLKAFNPAYALKLIIQQPSALLILGAVFLC
                     TTGVEALYSDLGHCGLKNIRISWFFVKTTLILNYLGQGAYLLNHSYNGTNPFFAIMPE
                     WLLFPGIIIATCAAIIASQALISGSFTLISVAISLNVWPKMKKKNPTTVRGQVYIPVV
                     NWFLFVGCIFVILYFQESSKMGAAYGLSVNLTMLMTTTLLMFYMMIKRINSYFIALFL
                     FTYYIIEGTFLIANLQKFMHGGFLTIILASVFFLVMYGWYNARKIRNSFTSFVKLSDY
                     SDLITGLSRDRSVPKYATNLVYLTHANSREEIESKIMYSMINKQPKRADVYWFLHVDI
                     ADEPDTFEYEVTHIVPRTVIKIDFRLGFKVDPKINMYFKQVLDEMEENREIDLVSRYE
                     SLRRYNIDSDFVFIVIDRIPNFDYDFTPHDKMLLNLYRFLRHIGVNDTKAFGLDTSNV
                     IIEKVPLLVEKSYKRVIKRRIVQNTLSLDGSQAN"
     gene            63602..>64165
                     /locus_tag="PK252_03960"
     CDS             63602..>64165
                     /locus_tag="PK252_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOT13898.1"
                     /translation="MGHKPISTKHATFAGIVMTLGIVFGDIGTSPLYVMQSIIGGDSH
                     VDESFILGALSCIIFTLTLQTTIKYVIITLRADNHGEGGIFSLFALIRRIFPWAFIFA
                     IIGGTTLLADGIITPAITVVTAIEGLELYSPGIQVIPITIAIIVFLFLIQQFGTHKVG
                     KSFGPIMFFWFLMLAVLGALQISSYPAI"
BASE COUNT        21391 a        12494 c        12127 g        18153 t
ORIGIN      
        1 aagcccttac aacagcatga tttctcattg tgccaccctg aacaactata ttatcgccaa
       61 gttcagaaac gtctttaatt ttgagtactt tgtataagca gtttttaact acagaataag
      121 ccaaacctgc agaaatatcg gcaacagaag ctccttcacg aagaaattgc tttacttttg
      181 aattcatgaa cacggtacaa cgtgtaccaa ggtcacaggg agcatttgca ttgcatgcca
      241 acgttgcaaa gttagctgca gtatagttaa gcgatttggc aaaaccttca atgaaagaac
      301 cacagcctga tgagcaagct tcattaattt caagcctgtt aatagcgcca ttttcaacaa
      361 agatagcttt catatcctgg ccaccaatat cgaggatgaa ggatacttta ggattaaact
      421 tttgagctgc ggaataatgg gcaatggttt caactattcc atgatctatg ttatatgcag
      481 ctttaacaag gtcttcacca tatccggtta cgctggatgc aacaatgtga atatctttct
      541 tggcactatt tgctaagaca agaaattcac taagcccttc agatattgtt tttaaaggat
      601 ttcctttgtt tcgtgagtaa aattgaaaaa cgatacgttc tttatcgtca atagctacaa
      661 tcttagtagt agtagaacca gagtctatac caataaaaca ttcgttgccc tcaagttttt
      721 caagtgttat actttccaaa cgatggtttt gctttagggc aatccattgt tcaagttcag
      781 cctgattgtc aaaaagctta tttaacctgc ctttagaaat actatgaggc tgtttgttaa
      841 tgctttcaat tttttcaatt aagctattca ataaaacagc tttttcatct tgtttttcgg
      901 ttaaagcaca tccaaaagcc ggtattaatg cagcattttc agaaataata taatcttcgt
      961 cttttagttt aagataattt acaaaagcct gacgaagtgc agggataaac gctaaaggcc
     1021 caccacagaa aaatattttt ggaagaggct ctgtaccccg tgaaagagaa gttattgttt
     1081 gtaaagctac agcatgaaat acagatgctg aaatatcggc tatgctaaca ttacgtgcaa
     1141 taaggttttg gacatcagtt tttgaaaaaa caccacaacg agaagctatc gggtaaattt
     1201 tccctgcaga caaagcctgg cggctcattt ctgcaatatc cacagctaaa atggttgcca
     1261 tttggtcgat gaatgccccg gtacctcctg cacagcttcc attcatacgg atatcaggga
     1321 tactgccagc tttaagaaaa accatttttg tgtcttctcc gccaatatca atgagcgttt
     1381 gaatttcacg gtgtttgtgg gagatataat ctgttgctgc aataacttcc tgagcaaatt
     1441 gcaggccata gcgttcagat acgcccatgc cggccgaacc agttacacaa acagcaatag
     1501 cttggttgcc aactacacag aaggcttttt taagtatgtc ggtcaaagca ctgctgatat
     1561 tggcgtgatg cctctgataa tctgaaaaca ggactttgtc ttttttctca tccagaacta
     1621 ccattttaat ggtagttgag cctatgtcga taccgcaatg aagtggctgg ttggtcataa
     1681 tatagggttc acttaaaata aaaacacaaa tataggtatt ttatgttttt taacaaaaag
     1741 cgagttgact atggatgaaa ggttgaactt agttggaagc gcaaaatctt ttgcaacggt
     1801 tttaaaaaag ccaataaaag gattattttt gttttgtaat gttgctaatt attctgttaa
     1861 gactaattga gcaggtataa aaataagtat gagcccttat tctgaccgta aagaagattt
     1921 tattataaaa cccgattatt tatctccagt taaagtttat agtatcgtca ggcctgctat
     1981 cgaaactggc tatgtttaca attttacctc agatagcggg ttattttatg aagtccggtt
     2041 tgcgcccaaa gtggacaata tacttggtat ggtggtcaat tttactgtgg atagcgatga
     2101 gtttgacagc gactatccgg taaccaacag aggcgaggta tataatatca ttgccacagt
     2161 tatagaaatt atcaggatat ttcatcattt tcacaacttt actacatcgt acgaattttc
     2221 tggggaattc aaagaaaacg aatcaaaaac tgagcataat gccagtatcc gttcccgtct
     2281 ttatttgcgt tatgccccaa tagtacttag tagtaattgg aaagcccaaa ttaccgggaa
     2341 taaagtatta cttaagaagg tgaagtaatg aatcccttat tagaagtaaa aaacctctgt
     2401 attagtttta acaattcagg caaagctgta gataatcttt cattttcatt gccagtaggc
     2461 caaacattag gcattgtagg agagtcgggc tcaggaaagt ctgttacagg attggctata
     2521 actcgtcttt tgcctaaaac cacaaatatt tcaggacaaa ttcttgcacc tcacaattgg
     2581 cttgataatg ctgctgatga ttatattgat ttacttcagt tacctgaaaa atcaatgatt
     2641 aagctacgag gtaaacgaat atcttttatt tttcaagaac cgatgacagc attgaaccct
     2701 tcaatgcatt gtggtaagca gatagaagaa atattaaagt tgcacaaaat agtagagaaa
     2761 agcaatttga ttgaaagaca attgcaattg ctcaaggatg ttcaacttcc aaaccccgaa
     2821 attgttttaa aaaaatatcc ccatcaactt tcaggagggc aaaggcaacg gttgatgata
     2881 gctatggcaa tggcagcaaa tccggacatt attattgcag acgagcctac aacagctttg
     2941 gatgttacgg ttcagtttga gatacttgaa ttgctcaagc aaatgaagca ggagcggaac
     3001 ctcaccatga tatttatctc gcacgacctg aatgtcatca gccatatagc cgataatgtg
     3061 ctggtgatgc gacatggcaa catgatggag caaggcactg tcaggcaagt gatgaataat
     3121 ccgcaacatt tatacaccaa agcattgctc gaaagtaaac cttcatttca ttcaaggcct
     3181 gttaaattgc ctacagtaga tgattttatt gatgaaaagc ctttttataa agaagaagaa
     3241 agtattgagt tacgaaaaga aaagcaaaat aaaatataca atgcccggcc gctcatagaa
     3301 ataagtaatt tgaatgtagg cttctcgtta aaatccttat ttaacaaaca acaaactttg
     3361 atattgagag gtattgacct gaaaatatgg aaaggcgaaa cgcttggcct tgtaggtggc
     3421 tcgggaagcg gaaaaactac attaggaaga actttaatgc agttaataga aggatatcag
     3481 ggcaacatta agtatgaggg aaaaaaaata aaagactttt cgaagaacga aatgctgaag
     3541 ttcaggcaga aagtccaatt ggtttttcaa gacccttatt cttcattaaa tccttatcag
     3601 accattgggc aatctataac tgagccaata attgtgcata atttgtataa aaataattca
     3661 gaagtacgta acagagctat agaactgatg caaatgaccc gcatacagcc cgaatggtat
     3721 agccggtatc cacatcaact ttcgggagga cagcgtcagc gggtagttat tgctcgggca
     3781 ttagcattaa acccagaagt gctaatctgc gacgaatcgg tatctgcgct ggacgtttct
     3841 gtacaagcac aagtgcttaa cctgctcaac gaactaaaag aaaagatagg gcttacatat
     3901 attttcattt cgcatgacct ggcagttgtt aagtatatgt ctgatcgtat tgttgtactt
     3961 gacaaagggc ttattgttga agataacgaa gctgacgaat tgtgccaaaa tcctgcacat
     4021 ccatatactc aaaggctgtt aagtgcggca aaaatggaat aaatattttc taacaataaa
     4081 agtttattta ttcatcaata aatatatttt tgtattctca agagtatcac attattggat
     4141 aggataattt ttaaaaagat aagtaaagac agggattgaa aaatgaaaac taaacttata
     4201 attgtttcag tcatttttat tggccttttc agtattttca tgctgaaagg gctgagttct
     4261 tatcccgaaa aaccaaaaat ggagctcaac ccagctcctg tgaaatctga attagaggca
     4321 ttatatgctt tatacgatag tgtaatgcgt gccgaaatgg attcgacagg tgttgttggg
     4381 gccagcgtgg ccattgttta caacaataaa atagacttcc tcaaatgtta cggagttaaa
     4441 aaagtaggag aaaccgattc gataaacccg caaacagtgt tcaggcttgc ttcggtttca
     4501 aaggctgtta caggcgtttt ggcgggattg ttggataacg aaggtgttat cagcctcaat
     4561 gataaagtaa ttgattatat tcctgatttt aaattaaaaa gcgctgtcaa tacaaataac
     4621 ttgcgtataa aaaatatcct gagccacact tcggggctta ctcctcatgc ttttgacaac
     4681 cttgtagagc aaaaaatgcc tttcaataag ctaatgtaca atttgagtaa agctgaaata
     4741 tctggaaaac ctggacaatt atatagttat cagaatgtaa tgtatagttt atttgacact
     4801 atagcttcgg ttaaaacatc aaagccttat ggagagctta tgtcggaaaa gattttcaag
     4861 cctttcaata tgtataatgc atcaaccagt tacgagtctt ttaaaaacaa cgacaatatt
     4921 gcctatccgc acaaatggac caaaaacggg tataagccaa ttcctcttaa tcaaggctat
     4981 tattccacca tatcagcagc cggggtgaat gccagcattt cggacatggc cagttttttg
     5041 ttaaagcttt taagaacaga cagtttgtct ttagactctg gtgtaatccg cactatattt
     5101 acaccacaag tggttactcc gctaaacagg cattatttta aggagtgggg aaaagttgct
     5161 tccaggcaat atggcattgg ctggaggatt atcgaatata aaggccatca actggcctat
     5221 catggaggct ttatacaggg ttacagggca gaaatagcat tgtgtcggga gatgaacgtt
     5281 ggcattgtgt ttttgaccaa ttcacccaac aaagcagcta ccataagtat ccctactttt
     5341 ttgaatatgt ttttcgacct gaaagaaaaa ggcgtattag ctgccaaata aaacagggct
     5401 ctaaagcagc ttatctgctg ttgttataaa attattcgaa cccaaatgat gaattgtctt
     5461 tgcgttcaga ttctcggaaa gcagacggcc tttataggca tctttttggc cgaaggcatt
     5521 gattacatca cctaatacta ttcctactgt ttcttgcatt actactgaat gcaggttgca
     5581 ttcgagataa gcaatacaat ccaaaggcaa agctattttc agaaccgggc tttcaaaggt
     5641 tgataaatgg agatgctcaa ttttattgac cttatggcca gaacaagagc caagttcttt
     5701 taccatttct acctgattag ctgaaggcaa agcaatgaca aatttttttg tatcaataat
     5761 attggcataa gttttatgtt gcaagtcact aacaaaaagc agacgtgtgg ttttgtcata
     5821 gtccaccggg caatgccatg caataggagc catattgtaa ataccttcag aagacacagt
     5881 gcatactata atcagtcctc ccgaattgag gagttgataa gctttttcat ttttgacagc
     5941 ttgaaaattt tccattatta gcatgagttc agattaaaca tttctatctt ttgcttaaaa
     6001 gcaaaagtac tacaaataaa atgctaaagg tcaaagattt atcgtaaatg ggctaaaaaa
     6061 gatttttatt ctcatcagga cattgcttgc ggaaccttta aatcatttat ttcttcccaa
     6121 agcttttgaa atagttaccg ggaatttctt acattgtcac ccaaaccgcc ccattaataa
     6181 agagtttgcg tgtttaggcg ttttataatt tttttcaagg ccattaccgg aaactatttt
     6241 tgaaaaaaac tccaatattg acattttggc aaaaagaatt gtttttataa ttttaaaata
     6301 tcatctttga ttaaatttac atttactaca agtaactgaa aatatactat tcatggcaca
     6361 ctgcataata aaaaacattt tgttcattac tgttcttttt ttaacgttcg catgccgaaa
     6421 cgaaagcaac tctaatgtta ccttaacaga agaagacaca cttgtcaata ctgaagaagt
     6481 agtggctaaa gcccgaagtg ttttttaccg gatgtattta cctaatgaga tgtataaggt
     6541 ttttgaaaaa gccggggcta tttattcgcc cgaaattctg aaccccgtag aaaacgtcaa
     6601 tttatacgag tcgagtacca aagccgccct taatttgggt gtttatggcg ttgaccttag
     6661 ctacaataag atttttgggc aaaaccaaaa aacattgctc tattttacag taatacacaa
     6721 actttcacaa cagcttggca tcccggacaa tattttttcg caagcattga aaaaaatgga
     6781 gcaaaacttg tcaaatcgtg actcattaac agagtatgcc acagatgttt tttcttctac
     6841 acataagttt cttaacgaaa acgacaggaa atcaacagca gcactgatta tagctggtgg
     6901 ctggattgaa gcgctataca ttgcctcaca aatatctgat gacgatgtag aaaacaaaga
     6961 aatcatagag cgtatagcat tccaaaaata ttctttacgt tcgctcatgt ctatgcttgc
     7021 caactatcag gccgaacctt cggtaaacag gtattatctt atgttcaaag cattgaataa
     7081 tgcatacaac gaatttgaaa tatactattt cccggatgat gtttcaattg atactattaa
     7141 caagcttatc aatgctaaaa aagtaaacat tgatttgccg gccgggaaaa ttgaaaaaat
     7201 caaaaagatt attgcccaaa tccgtcagga gattaccatg tagcctcaac gtattgacat
     7261 aatttttgaa gccactgctt ttgcagcatc agagcccaaa aaagctacaa gccctctgcg
     7321 cagctcttct gcaagggcag gcaaatgaac cggtgcaata gattcttcat ttattccaag
     7381 cttagcggcc tgactcttga gggtaccttt tgtcatcata tctccaatca aagggactag
     7441 aatttcatat attttttgcg cgtatatatt agtatatgag ctcataatta ttgtgcatta
     7501 acagattcaa ctaattcttt ttgatacaaa ccggcaagac caatgaccag gtaacctaca
     7561 tgccataaaa catcaataag gttgccattt ccataaatat ccagccaacc taaataagca
     7621 tataacatat cggccattgt aaagcaaaca aacccgatgg acaacatgcg cattggcaag
     7681 gaaatagcag cccgtccaaa aagcgtagtt atgtaaaaca gtataattgc aggaataact
     7741 gttaacaaat ctgctattgg ataatacata taaaaaagtt ttgtaatatc atctgattca
     7801 ttatcagaca aaatgggaat aagcagaaaa taggccattg ccacaaaaac gacgaaagaa
     7861 ctggcagcaa taatggcata aactttcatt tttcccagtg gaaagccact ccttttataa
     7921 ccaaaaaaca tcataaacaa acctgcaaat aaaggaatat aagctgtaca ccagaaaata
     7981 tcagctaaag aagggaaata ctcattcata tcaattttaa atccaatttc gagaatggca
     8041 taagtggttt ctgccaaaaa atcaaaaaag ataccaataa aaataagcac ccagcttacc
     8101 cgggcaaaat ctatttcttt aaaagctttg aaggtatgat acaaacatac aacggcaata
     8161 aatgatgtca atacgggcaa tgtatcactt acatactgca atacagaagg cccgccccac
     8221 tgtcccagat aatataccac atttaaaact gttaatacag caataaaaat caaaagcaat
     8281 ttagactttt tcatacattg taattgtgtt ggttttatat ttaaatgtaa gccttttttt
     8341 tcaaatattc aagctcaaga taagattagc cctttagata ataaaactta agcattagcc
     8401 tatcagtttt tttcgtatat ttaactaaat gtaagataat tgcatgtttg ttttacttaa
     8461 tacaaactca aaatcgaaaa ctatgaaagc tatatttttc gcctctttct tagccattgt
     8521 cttggttaat acctgggcgc aggaaaaaga ttcgtcaggc ctgagtttga atcttggagc
     8581 tgatgttgta agccgctata tctggcgtgg ccaggccaat agctcctcac cggccatcca
     8641 gccaacttta agccttaatt ataaaaacat aagctttggc agttgggctt cctataccac
     8701 ttccaataac gacttacagg aggttgatct tttcctgacc tataaaattg gaggatttac
     8761 agtagggttg aacgattact tcatgtcatc agatagtttg gacgacctat attttaattg
     8821 gaaaaacaaa gaaacaggcc atacgttgga gcctttcatt acttattcag aaattgctgg
     8881 tacgccatta agcctcactg ctgcggtatt tgcgtatggc gatgatttag atgaaaatga
     8941 agacaactat tattcaagct atttggaact tagctttgcc aaaaccttag gaaacattga
     9001 ctacaacatt ttttgtggcg ggactttcgg gaaaggttat tatgccgatg atatgaacat
     9061 tgtaaatgtc ggcatcactc tgaccaagac aatagaaata acgccaagct tttcactgcc
     9121 ttgtaatggt tcatttgtga taaacccaag taccgaaaat gtttttgttg tttttggaat
     9181 aactttttag cctgaaactt aaatactata attacattta aaccacccct acccttcctt
     9241 atacaggagg ggaaatattc catattacta aaaaaattat ttcaacttat tttcaagctc
     9301 ttcaatattg ataatggtca ggatggtttt gccttcaggg caataattat ggtttttaca
     9361 ggcgaatagc ttgtcaataa ggtcagacat ttcctcactg ccaagcgctc gtccatacgg
     9421 tatggcagat gctttgctca atgccagggc tattgtttct tttaccgaag tcccgctttg
     9481 cgaaggagca tggtgcaatt cggcaagcaa atcatccacc agcttttgcg ggttggcctg
     9541 ctccaaagca gccggaaagc cattgacaac aactgagttt tgcccaaaat cctgaatgtc
     9601 aaaaccagcc tggttgattg cagcagaata ttccattatc agctcatggt ctgcaggaga
     9661 aagctcaacg acctgcggaa aaatttcttt ttggactgca tttgcacttt tttcaatatt
     9721 ttcaagatat tgctcaaaca agatgcgcaa gtgcgccctg cgttggtcta ttaacaaaag
     9781 gccggattta gaaggggtta ggatatattt attcttaact tgaagaaatt gttgctgctt
     9841 tgacacctgc acgtcatcag cccctttatt ttcgacaggt tcaaaatttg ggattcgtag
     9901 ctctgtctta ggttcatttt caaacccact gtacagatct tgccagttat tgggcaaaga
     9961 acgcgaatga cttgaaaaat cagccctcac gcttacgctc tttttttgct caggtttaaa
    10021 tgggtcaaaa gttgcatcaa tatctatttg aggcagattg acatctgtag ctcgcgaaag
    10081 tacaggaatt tcaaccgaac ggtctatttc aaaatcaata gatggcacaa tgctgaattt
    10141 ccctaaggat tccttcaccg tagcatgcaa aatctgccat acagcctgct cgtcaaaaaa
    10201 ctttatctca gtctttaccg gatggatatt gacatcaata ttggccgggt caatggtaaa
    10261 acaaatgaaa taggaaggct gcaattctgg taacaaaatc ttttcataag ccttgataat
    10321 ggccttatgc aaaattggat gccgcatgta acggttgttg acaaagaaaa actggtcggg
    10381 ctggctcttc tttgccacat ctggtttggt aatgtatcct gttattttta tgatagaagt
    10441 ttcgcaattg acatctacca gcgattggtt gatatttttg ccaaaaagct gaataatcct
    10501 ttgtctgaaa ggcgaagtat aaagctttaa aacctctgta ttgccatttc tcagttcaaa
    10561 ctggatatga ggaaacgcca atgctacttt ttgcaactcg aacacaatga gcttaaactc
    10621 gtaattgtca gttttcagga acttgcgacg aaccgggatg ttataaaaca ggtttttcac
    10681 cataaaattt gtacctgtgg cacatgaaac cggtgtttgg cctaatacct cagaagccgc
    10741 aatgtttatt tctgttccca gctcatcctc ctgacgctta gtacgcaaaa caacctcggc
    10801 cacagcagct atagaagcaa gtgcttcgcc cctgaagccc atggtatgta tatcaaacaa
    10861 atcttcagcc cgcttaatct tagatgtagc atgacgttca aaagacatac gggcatcagt
    10921 ttcgctcatc cccttgccgt tgtcaataac ctggattaaa gtacggcctg catccttgat
    10981 aattactgat atttgtgtag ccccggcatc aatagcattt tcaaccagct cctttactac
    11041 cgaagcaggg cgctggataa cctctccagc cgctatctgg ttggcaacac tatcgggcaa
    11101 aagcgctata atatcgggca tatttcagat ttttatgcaa atttacattt attgcaacat
    11161 ttgccaacag ggggtaaagg catatttatt aactttgcca ttatgctgga aaaaacaagg
    11221 gctataacac tacatgggct taaatatggc gacacgggct tggttgtaca tctttatacc
    11281 gaggctcatg gccgccaatc atattttgtg aaaggtgctt tttcaaaaaa aagccagata
    11341 aagtccaacc tttttttccc attgtcatta ctggaactgg aagtatatca caagccatca
    11401 tcacagttaa ataaaataaa agaagccgta ctaactcctc aactgcttac gattcatagc
    11461 aacccataca aaaatgccat tgccatattt atttcagagg tgctttacaa agttttgcgc
    11521 gaagaagcag ccaatgacag tatgtttaac ttcttgttca attccgtttg catgctggac
    11581 ctgcatcacg actcgccttc caactttcac ctcgttttca tgtgccttct ggcaaaacag
    11641 cttggctttt tcccaacaaa taactactct tcggtaaaca aattttttga tttgcacgaa
    11701 ggcttttttg tttcacaaca cccctcacac agacagtgca tgggcgcagg tatgtctgaa
    11761 atattctcag aacttctcgg tacaaattat gaaaatagtg cccatttgcc catcgacagg
    11821 atgatgcggg cttcaatgct tgatgctatt gctacatatt tttacttaca tatgcacgga
    11881 ataggccagt ttaaaagcct ccaggtgttg cacgatgttt ttgcctgatg tttttgcaag
    11941 aaattttcaa aatgatacct ttacaaccat aaccaaaaat actaaatatg attgcaagga
    12001 acgcatttct ttggattata gcatttgtaa caatttcggc ctttcagcag gccaaagcac
    12061 agaaaaccat tacttttcct tcaaaagaca gccttaccat cactgccgat tactacgaag
    12121 ctgacccaaa agcgcctttt attctgctct ttcatcaggc aggttccagt cgtggcgaat
    12181 ttactgaaat tgccccccgc tttgtcaaac tcggctacaa ttgtctcgct gttgaccttc
    12241 gttcggggaa tgagacaaaa tacgttatca accaaactgc tttaaaagca aaaagccaga
    12301 aaaagccaac aaaatactta aatgcagccc tcgatatgca agcagccatt gaatatgcaa
    12361 gcaataaaag ccgaaagcct gtagttgtgc ttgggagctc ttattcggcc tcacttgggc
    12421 tgttattagc aaaaaccaac ccgcaaatta gcgctgttat agccttcagc ccgggcgagt
    12481 atttcgaaaa agaaaatttt acaagggata gtattgcagg ccttgacatt cctatatttg
    12541 cagcatcggc aaaagatgaa tacaagtacc ttacagcgct tttcggcaaa gtaaaccgta
    12601 ttaactatac gctcttttgc ccttcaaaag gcaatggcga acatggcgct aaggcgcttt
    12661 ggacagaatc tcctgaaaat ggcgaatatt ggctggcatt aatgctgttt atcaatcaaa
    12721 taaaggattt ttaatttcac tgcttcataa aacaatataa ttatgttatt caacaccctg
    12781 cattttttga tattcttcgt tgttgtattt gtccttttct ttacactgcc ccataaattc
    12841 agatgggtta tgttgttagc cgccagctgt tatttttaca tggccttcat cccaatctat
    12901 attctaatcc tggcattcac aatagtagtg gactattttg cagggataat gattgaaaaa
    12961 tcgcacgata aaagaaaaag atggttttta atagccagtt tagtggccaa cattggcata
    13021 ctggcatttt tcaaatatta caactttctt aacaacaacc tttcattatt gttggggaaa
    13081 tttggatatc ctgacccaat acccgccctt gaaatcattc tgcccatagg gctttcgttc
    13141 cacacgtttc aggctatgag ctataccatt gaggtgtaca ggggaaaaca aaaagctgaa
    13201 agacattttg gcatttacgc actctatgta atgttttacc ctcagctggt tgccggccct
    13261 attgaaaggc cgcaaaattt gctccaccag ttccacaata agcattcatt tagcatacaa
    13321 aacttatctg acggattacg gttaatcatt tatgggttgt ttttaaaaat ggtcattgcc
    13381 gataatgttt ctccttatgt caatagcatg tatgccaatc cacaaaatta cagctctacc
    13441 gacattatta ccgccctttg tttctattcc atccaaatct attgcgattt ctgtggttat
    13501 tctttaatcg ccataggaag cgctaagtct atggggttta atttgatgaa caactttcac
    13561 acgccatatt tctcaaggac agtatctgaa ttttggaaaa gatggcatat ttcactctct
    13621 acctggttta aagactattt gtacataccg cttggcggga accgtgttgc attgccgcgc
    13681 tggatgttta atatatttat tgtctttgct attagcgggc tttggcatgg ggccagttgg
    13741 aactttgtga tatggggcgt attgtggggc ttgttaaatg taggggaagt tttatacaac
    13801 aagcttttta gcaggcctga agaaaaagga gactttaaaa tctggcatag catccatgct
    13861 gctaagatgt ttataatagc tacctttttg tgggtctttt tcagaagtga gaacatggag
    13921 aaggtcaaac ttcttgcaaa atcgattaaa cacaactttt cattaaacaa tagcttaaat
    13981 gtaccacatt tggtttgggc gttgttaatt gtatttcttg ttttcgaaat tatgttctac
    14041 aaccatcgtt ttgacaaatg gtgcgaaaaa agggacccga taataaaatg gtcggtttat
    14101 atcatcctta tctcgtcaat tatcatgttt gcgggcattg aggacattcc atttatttac
    14161 ttccagtttt aaaaattatg aaacgcccat gattgtaaac aaacaaaaag tcacatgagt
    14221 aaaatgcggg cgttgtacca tcccttaaaa ataaaatttt attatgataa aaatagctaa
    14281 atttataatt cccagggttt tagtgcttgc cataacatta atcgcagcaa atttcatcta
    14341 cacaaaattc ttttttgaaa aagaccttca aatgcatgct gatatgatta accttacccg
    14401 tgggaaagac tcagcaagag tgatatatat gggagaatcg tcaaatttca cttcgcacag
    14461 tgatgacaaa gacccccgaa gcatatccgg tatgctatgc gactatttcc cggggctaaa
    14521 cattcaagat atttcgcatg gggcattaca cgcatataat tattatattt ttttgaaaac
    14581 aattcagaat aattctaaac tggaaaccat catcataacc ttgaaccttc gttcttttga
    14641 tgccaattgg agatattcag acctggaaac ttttctccaa aaacaaatgg taatgataga
    14701 ccctacttat cccccattgt ttaggcgttt tttgctgggc tttaaaggat atgacaataa
    14761 aacagtaaaa gaacgggaac gccaattttt acatgaatgg gaaacaacag tactgaaagt
    14821 ccccttccca cttcaatata ataccgtaag aaaatgggac aaaggaatag caacaaacgg
    14881 gtatatagat gtttcaggca atagaaatga agattcaaca attctcgcct gtcattacgt
    14941 aaaaacttat gcctttcaaa tagacaccaa caaaaacccc cgtattaaag attttgacaa
    15001 cattgttaaa ctggcaaaac aaaggggttg gcatgttatt tttaacctga tggccgaaaa
    15061 cactcaaaaa gcggacaaac tcgtaggaaa agaacttgtt tacctgataa agcaaaatag
    15121 agatttatta gtttcgcgat attcaaaaat gggggccatt gttgtagata acttagaatg
    15181 cgtcaatgac agcaattata ttgatcaaaa ctggactact gaacattata aagaaacagg
    15241 gcgcaaaaca attgctaaaa atttgtcgga agcactaaaa accatatacc cacaatacta
    15301 taaaagtatt gttgcagata ataaatagat tataattaat gaaagtaaaa tttatcattc
    15361 tccgcttcag ctctattggc gacattgtgc taactacccc tgtcatcagg tgtttgaaag
    15421 aacaggttga aggggcagaa attcattttg ttacgaaaaa aaagtttgca tcggtagtgg
    15481 ccgacaatcc ttatattgac aaaatccacc ttctggatgg taagttaaaa agccttattg
    15541 aagagttacg ccaggaagaa tgtgaccata tcatcgattt gcaccataac cttcgtacat
    15601 atatcgtaaa aaagaagctt gggttgtttg cattttcttt tgacaaaatc aacatccaga
    15661 aatggctgat ggtacaattt aaaatcaata aactacctca aaaacatatt gttgaccgct
    15721 accttgagac gtgccgcctt tttgatgtaa ccaatgatgg caaaggcctt gattttttta
    15781 tcaatcctaa aaatgaaatt gaaatttcgc agctcccatc atttttacaa aacggataca
    15841 ttgcccttgt cattggaggg atgcatggca caaagcaaat gcccaatgca aaactcgccg
    15901 aactaagcaa taagctgaat ctgcccgttg tattgcttgg tgggcccgac gattctcaag
    15961 ccgcagaaag cattattaat gaaagtaaaa atgccaatat cttcaacgcc tgcggaaaat
    16021 acaacctaca acaatccgct tcgatagtaa aacaagcaaa gcttgttatt gcgcacgata
    16081 caggactgat gcatattgca gcagcattta aaaagcgtat tatctctata tggggcaata
    16141 ccattcccga atttgggatg tacccttatg taagtgatga gaatcaggct atttttgaag
    16201 tcaaaggtct gaaatgcagg ccatgttcaa agattggcta caagaaatgc ccaaaaggcc
    16261 accacaattg tatgaacctt attgatgtca acaaaatagc cgagaaagct aatagttgga
    16321 tttaaaacat tcatcttacc cggaatgatt actttttttg ctttaatatt agccatagta
    16381 ctccaatttg caggggcaat agtagctatt agtaatatca ggcgaacaaa atacagtatt
    16441 tcgtggatat taatctctct gggctttctg gtgatggcca ttcagcgatt tatcgaattt
    16501 ataccctacg tttggcgggg ctgggaaaaa gacgtagcaa caatcaatac atggttaggg
    16561 attatcacct ccattcttat tgctgttggg gtaatttata tacgcaaaat ccttgatttt
    16621 ctgcggaaag tagaacgaga aaagcaggaa tcgcagaaaa agattttgaa cgctgttatc
    16681 caagctgaag aaaaagaacg ccgccgcctt gcaaaagagc tgcatgacgg catggggcca
    16741 ttgctctcgg cagtaaaaat ggccgtttcg tcactggtaa agacagaaac caactccaac
    16801 aataaactta tcatcgaaaa caccgaccag atgataacag aagctattcg cagtttaaaa
    16861 gaaatatcaa acaacctgag ccctcacgta ctggaaaact ttggattggc aagcgctatt
    16921 aaatctttct catctaaaat aagtgctaca agggctattc agattacatt cttttccaac
    16981 atgtttgata ttaggctcaa tagcaatacc gaaatcatca tttaccgtgt agtgtgcgag
    17041 ttgataaaca acactcttaa acatgccaat gctcaaaaca ttgagattga gctgaaacaa
    17101 catggcaaaa tcattacttt gctatatact gatgacggct gtggttttga taccagcaca
    17161 gtgttacgaa acgaaaacaa aggaatgggg ctttcaaaca ttattaaccg tataaaatct
    17221 atcaaagggg ctgtaaatat tgagagtagc cacggcaaag gtttgaaagt aataataatt
    17281 gtaaatactt aagaagataa aggttctaca tggattgata cttctatatt ttttatcttc
    17341 tccataatat ctttctcaat catatcgcac atttcatgag ccatacccac cgaaatatcc
    17401 ggagcaactt cgaggtgcaa ctccgcaaac ctgtaatgac cggccttacg agtacgcaaa
    17461 gcatgaaaac taagattgtg tgatttaatg gaatctttaa taacctctac ttcactttcc
    17521 gagagagaag catccaataa cggggcgtaa gccgctctta aaaggtgtac agcctccttg
    17581 actattagtg tagcaacaaa aacagcaatc acagagtcta aaaagtgcca accagtagcc
    17641 caaataagca ataacccaat agcaacaccc aaagatgtat aaacatcggt cttcaaatgc
    17701 agcgcatctg cttccaatgc aatagaatca gtctttttag caactttata taaatatctt
    17761 gaaacaaaga aattggcaac agccgaaaca aacatcacaa ccgagccaag cccaatagac
    17821 tcaataggac caggatgtaa aatctttaca acagcttcat aaataatcca tcccgatgct
    17881 aataatataa gtaaagcctc aataataccc gatacatttt catacttccc atgaccataa
    17941 ggatgacctt tatcagccgg ggtatctgaa atacgaacag caaaaaaagc aatcactgca
    18001 gccagcaaat ccattgccga gtgaattgcc tcagacaaaa tactcaccga acccgttata
    18061 aatccagtaa ttaatttgaa aattaccaac gatgtattgg aaaaaacgga aattccggca
    18121 gctttagttt tttcgttcat caaagctttt ttagattgtt ttttgcaaat ataaaatcta
    18181 atttaaaaag aataattgaa tttgcaaaag ctttacttaa acacctctgt ccgcttctgg
    18241 gctatatacg actctttagt attaacaatt atcgtagaag aataaggcaa ctctgagcgt
    18301 tgaagtttat tagtaggcct tacaatgaaa gtcctttccc cagccttgat tacaacaggt
    18361 attgagaatc cttcaacagc attgccccat ttgtaataaa aattgttttt gccagtattt
    18421 tggtcatacg aaaactccca aaggagtatg ggacaaaccc tcgaatacaa gtactgattg
    18481 aagaatgtgg taaaatcttt accagtcttt tcattcacca gcttcataaa agcctctgta
    18541 gtagcaatac cataagcatt ctgcaaataa aaggttttta aaatgtccct gaaaatagaa
    18601 tcattgttta ttgagtttcg taaggtatgc aatgtcaaag cccctttcat atatacatcg
    18661 gtatcacggt aatcccaaaa gttaacagca taagggccta taacaggctt tttgttttta
    18721 atcaacatgg cgtaaaacct gagtaattta aaataatcat ctttcccctt tgctgcctcc
    18781 aaaaaaaggg cttcagaata agtagcaaaa ccttcatgta tccagacatc agcataatct
    18841 gaggccgata cgctattccc ccaccattca tgggccgttt cgtgcaatat aatatggtcc
    18901 gtcccaatcc aaggtgctat tttataagca ttaccataag caatagcaga ctggtgttcc
    18961 attccctcat aaggactttc tacaagcttg tatccatctt taggccatgg atatggcccg
    19021 taaaattgct caaatttgct gataatacta tctactatca aaaaatggtt ctgggcaatt
    19081 ttataattgt cctttaatac ataaaaatcc atgtcgaaat ttgaaaacct gctactgtaa
    19141 atcctttgga acttggcaaa gttgccaaga taaaatgtaa aattgtaatg attgatagga
    19201 taatgaactt tccaggtata agtaacctca tcgccaatag cggtagaatc aatcatcacg
    19261 ccattagaaa cacaataaag acctttaggc acaataaaat gtgacaatat gctgtcaggt
    19321 tcgtcaggga gctggtcttt tgatggccac caaagactcg aacccgcaac ttcacaagca
    19381 actccggccc aggtattccc atatttatca ttcttccaaa ccatgccacc ttcccaggga
    19441 ggacgcttag ccacttgtgg tttgccatga taatacatag tcaactgcaa tctctctccc
    19501 tttgctacct tttgcggggt ataaataaat acagctccat gttttcgaga aaaaatgaga
    19561 ggtttaccat tcatcaaaac actatcaaaa gataaattgg ggtacaaatc tacctgtaac
    19621 atttcaaaat cactcaatgc cttgcagtgc atgattacct ttccgccaac ttctttagtt
    19681 tgaggttgta ttcgaataaa caactcataa aacgtaacat catagcaggc tcgccgggga
    19741 gaaatggccc catgcaaaga gtctgcctca gtaaactttg gaaatttata agcaaaacga
    19801 ggcgttttat gcgacatatt aatccctaca aaagagcatg aactaaaaag taaacagcaa
    19861 aaacaacaaa aaaggccctt ttgaacaaaa ttaattttca ataatcggat aacttgagct
    19921 gcaaaaacaa gtgagcttgt cccgacaaat ggcaatccaa acgcgtttgt gtttacttct
    19981 atacatgccc gtttcatgcg aaaaattctt acaacgtcga aaatacctgc aaatacgatg
    20041 cagtgcacaa ttcaaaatct ttaaatgcgc cacaatatta atgaaagata attagaaatt
    20101 tggaaaacaa taagtttttt tgtacatttc gccaaaatcg ttgtaaaacc taaaacacat
    20161 cacatgaaaa agattatatt cttagtttcg ctggttacaa tagctttttg gggttgcaaa
    20221 aacaattcat ctgaatatat aaacaaactg gaacagaaga ttaaagaaga ttcgctgaaa
    20281 aatgtagcag aaatgcagac cttaaaaagc gaaatgcagg cacgccttga ttcaatgcaa
    20341 caagcttgtg gcgattgcat tggatcgttc tatgttgtaa ccggaagttt ccgtgaacca
    20401 caaaacgctg acaattatct ggctgagatg aaaaagcaag ggtttaaagc tcaaatcatc
    20461 gttgccccta atgggtttca tctggtttgc atctattgcg gacaaaatct caaagacgta
    20521 ctcggagctc tccaatctgc ccgcaacaat gtacaacctg aatcgtgggt gtatattaaa
    20581 taatatgtgt ttttatattt attttatcta ataccttgca aattagaaaa aaaaacatat
    20641 aaatttgcaa tcgaaatatc attcagggaa taggggacaa aaagtcccct attttattag
    20701 ctatatgaac gtagaacaaa taaaaaacat agttgaagaa gccatcgaca aatctaaact
    20761 ttttgtcgtt gaagtaaatt gcagtccttc caataaaatc actgttgctg tggatgcttt
    20821 tcacaacatc acacttgacg aatgtgtatt tatcagccgt gccattgagt caaagctcaa
    20881 ccgcgatgaa gaagactttg aactggaagt aacctcaccg ggcctttcgc agccatttaa
    20941 agtacatcaa caatacctta aaaatcaggg caaagaagta gaagtgcttt tgaaagacgg
    21001 agaaaaacta acaggcatat tacaaaacgt ttcctccgat ggaatcgaat tagcgctttt
    21061 tgcccaaaag aaaaaaagca agaaacctcc tgtagaagaa cctgagaaag agttctacaa
    21121 gtttgaagag ataaaatgga ctaaagaaca tataaaattt taatagtact ttgttgaaac
    21181 cagaaaatta ctgatatgga aaacgcaagt cttatcgaaa ccttattgga gtttaaagag
    21241 cagaagaaca tcgatcgtgc aaccctgatg agggtacttg aagacgtatt cagggcacac
    21301 cttgtaaaac attatggcaa cgcagacaat tttgacatca ttatcaacat tgacaaaggg
    21361 gactttgaaa tttggcgcaa ccgtcatgta gtagcagatg atgaacttac cgaccctgtt
    21421 agccaaattt cgctgacaga agcccaaaag atagacgaag attatgaaat tggcgaagat
    21481 gttaccgatg aagtaaaact atcatcgttt gaacgccgtt cgatattatc attgcgccaa
    21541 aacctcaacg cccgtatcag cgaactcgaa aagagcaata tttacgcaaa atataaagac
    21601 agaattggcg aaatcatcac cggtgaagtt taccaggttt ggaaaaaaga aatacttgta
    21661 ttggatgacg aagaaaacga actttcgttg cccaagactg aacaaatccc gggcgaccat
    21721 ttccgcaaag gcgataccgt aagggcagta gtttataaag tagaaatgcg caataactct
    21781 ccacaaatca tcttgtcacg tactgcatct actttcctcg aaaggctttt cgaacttgaa
    21841 gtcccagaaa tcaatgacgg cctcattact atcaaaaaga ttgtacgaat gccaggggaa
    21901 agggcaaaag tagcagttga atcttatgat gaacgcattg accctgttgg ggcatgcgta
    21961 gggatgaaag gctctcgtat ccacggcata gtacgtgaat tgcgcaatga aaacatagac
    22021 gtaataaact ttacaaacaa cattcagctt tttatccaaa gagcgttgag ccctgctaaa
    22081 atcaactcta ttaaaataat tgaagaaact aagcatgccg aagtatatct tcaccccaaa
    22141 gaggtatctc tggctattgg taaaggaggg ctcaacatta agcttgccag caagcttaca
    22201 gggtatgaaa ttgacgttta tcgtgagtca gaacaggacg aagaagatgt taacctcgac
    22261 gaatttgctg acgaaattga cgattggatt atcgatgagc tcaaagccat tggttgcgac
    22321 accgccaaaa gcgtattaga aattggtgcc gaagacctgg ttaaacgtac agatctggaa
    22381 gaagaaacag tgaaagaact gatcaggatc cttaaagctg aatttgaata agcacataac
    22441 aaaaaagaag gttgatataa atttaaatat cagttattta taaaataaaa aagaaaagtt
    22501 acttttgttc cttgaaaaac aaataaaatc aaaaggcaac agtatcaatt tcgagtatca
    22561 ggcaaagaaa acaaaattga attttagcag cactataata tgagcgacat taaaggagta
    22621 cgacttagta aattagccaa agaactgaat gttggtataa acaccattac cgacttctta
    22681 aaaaagaagg gacataccat agaacatgat cccaacgcaa aaatttcgcc agagctggta
    22741 accttacttg aaaaagagtt tagcagtgag attagtgcaa aaaaagaatc tgaaaaagtt
    22801 gatttgaaag gcatacgtga aaaaaaagaa actattgtcc ttcaagaaaa gcaccagtca
    22861 atagataagg ataataatca agaaactgaa actgctttaa gcgaaagcaa agaaaaaatt
    22921 gaacttaaag ttgttggtaa aattaacctt gatgccccta aagttgaaaa gtctgctcca
    22981 aaggtaaatg ctgaagaaag taaaaaagaa attgttgtca acgaaaaacc aaaaaatatt
    23041 gaaaaaactg ctgctaaaat cgaaaagcct gtagatatcc ctcttgaagt gcctaatgaa
    23101 attgaagctc caaaagttgt tggccgtatt gacctcgaca atatgaacct caagacccgt
    23161 ccggataaaa aaacaaaaga agaacgcatt agagaagaaa aagaaaaaca aaaagaagcc
    23221 agggcaaacg taactgctaa aatagcagaa ccccagaata aaagcgaaga ggtaaaagca
    23281 gctaccgaag aaatagaaga agaaatagaa gaaacctctg aatcagcaag ccaggaagat
    23341 ttaatccgca tgccaattga aaaacttaat ggccctaagg ttttagggaa aatagacctt
    23401 ccttcagaaa aagaaaaaaa acttgtaata tcttcaagct caattgacag cgacaaattc
    23461 aaaaagaaaa aacgcaaacg gataaaaatt gaagggcaaa aagtaagtgt agtccctgag
    23521 caaaaagctc ccgcaaaacc tcttgaagta aaactcaaaa aagacaagaa aaaatttaaa
    23581 aaacctttca ggcctgaagt aagtgaagaa gaagtttcta aacaaattaa agaaaccctt
    23641 gcccgtctca cctcaaaagg aaaatcaaaa acttcaaaat accgtcgcga aaaacgtgaa
    23701 gaaatccgtg aacgtcaaca ggctgagtta gaactgcaag aaatagaaaa aaacatcctt
    23761 aaagtaacag aatttgtttc ggtaaacgaa ttggccacta tgatggatgt tccggcaaca
    23821 aagcttattg ctacatgtat gagccttggg ctgtttgttt caatcaacca aagattagat
    23881 gcagaaacca ttgtattatt ggccgaagaa cacaattaca aagtagaatt cattagtgca
    23941 gagattcagg aagcaatcaa agaagaagaa gttgacaccc cggacaagct tttaccaagg
    24001 cctccaattg taacagtaat ggggcacgta gaccatggga aaaccaaact gcttgacttt
    24061 atccgcaaca ccaacgttat agctggagaa gccggaggta ttacccaaca tatcggagct
    24121 tattcagtac aggttgcaga tggcaaacaa atcacatttc ttgatactcc gggtcacgaa
    24181 gcatttacag caatgcgcgc ccgtggggcc aaaattacag acattgctat cattgtgatc
    24241 gcagccgatg atggcgtaat gccccaaacc cgggaagcaa ttaaccacgc ccaggcagcc
    24301 ggcgtaccaa ttatttttgc cattaacaaa atagataaac ctacggccaa ccccgaaaaa
    24361 ataaaagaag aacttgcagc catgaacctt ctggtagaag attggggagg taaatatcaa
    24421 tctcaggaag tttcagccaa atctggatta aacatcgaca agttacttga aaaagtgcta
    24481 cttgaagcag aaatattaga actcaaagca aacccaaata aaaatgccaa aggtacagtt
    24541 gtagaatcaa cgctggacaa aggccgtggg tatgtagcta ccatacttgt tcagagtggc
    24601 acattaaaac aaggcgacat agtacttgca gggaccaatt atggaaaagt aaaagccatg
    24661 tacaacgaac gtggcaaaaa aattgacgtt gcagggccct cgacaccggt acttattttg
    24721 ggtttaaatg gtgcgccaca ggccggtgat gagtttcatg tacttaacga tgaaaaagaa
    24781 gtaaaagaaa ttgctgcaaa acgagaacaa ttacaacgcg agcaaggctt acgtaccaaa
    24841 aaacatatca ccctcgacga aattggccgc cgtattgcta ttggcaactt caaagagctt
    24901 aacattattg taaaaggtga cgtggacggt tccatcgaag ccttatcaga ctcgcttatc
    24961 aagctttcta ccgaagaaat ccaggtcaac attatccata aagcagtagg ccagatctcc
    25021 gaatcggata tcatgttggc tgttgcatcc aatgctattg tagtagggtt tcaggtacgt
    25081 ccatcaatga gtgcccgacg cctggccgaa aaagaacaaa tcgaaatacg cctatactct
    25141 atcatctacg atgccattga agaaattaaa gatgctatgg aaggcatgct ttcgcccgaa
    25201 atcaaagaag aaattgttgc cacccttgaa atacgcgaag tatttaagat ttcaaaagtt
    25261 ggtgctgttg ccgggtgtat ttgtaaagaa gggaaaatac aacgcaacac caaagttcgc
    25321 gttatccggg atggtattgt agtttacacc ggcgagcttg cctctttgaa acgtttcaaa
    25381 gatgatgtaa aagaagtact cccaggccaa gattgcggct taggcatttc caacttcaat
    25441 gacataaaag ttggcgatat tatagaaggc tacaaagaaa ctgaagtaaa gaaaaagctg
    25501 taaaaaattc aacttaagaa tatcataaaa aaacgccttg actcaaaagt taaggcgttt
    25561 ttttatggga aattacagcc tgctgatgag attaatttgg gtttaaataa acatttaaaa
    25621 attatatata tatttgcttg ccaagcaaaa gaaaccataa caatgaaata taatgccaat
    25681 taatgttttt gtgccaaaat tcagaactga tgaaattttg gaggaaataa aagaatgtct
    25741 cgaaaaaggt tggacaggat taggttttaa aactgtagaa tttgaaaacc attggaaaca
    25801 gtatactggt atgcccaatg ctcacttttt atcatcaaac acagcaggcc tgcatttagc
    25861 attaaacata tttaaagagc aaaataagtg ggcagatgga gatgaggtta tttctactcc
    25921 tttaactttt atttctacaa accatgcaat agtatatgaa aggcttacgc ctgtttttgc
    25981 agatgttgat gaatacctat gcctcgatcc aaaatcagtt ttaaaggcca tcacccctaa
    26041 aacgagagca ttaatatttg ttggcattgg cggcaacagt ggacaatacg aagaaatagt
    26101 caaaatttgt aaagaacata atataaagct catattagat gcagcccata tggccgggac
    26161 caaactcaat ggcaaacaca ttggtagtga tgctgatgta gttgtgtata gttttcaggc
    26221 cgtaaaaaat cttccttctg ctgattcagg gatgatttgt tttgctgatc agcaattgga
    26281 caatttagcc aggaaaaaaa gctggttggg catttcaaaa gacacatacc agcggttcaa
    26341 taaaaacgag ggtagttaca aatggaaata tgatgtcgaa tctatagggt tcaaatatca
    26401 tggcaattca attatggcat caatgggctt ggtacaactc aaatatttgg acgaagacaa
    26461 tacttataga agaaaacttg cgtctttata cgataaaaaa cttgaaaata taaaaaacat
    26521 tcaaattatc aaagttgccc ctaattgtga aagttcaagg catctttacc aaatttgtgt
    26581 cccgaatcgg gatgaggtta tgcaattttt ctatgacaat ggtatttatc caggcgtaca
    26641 ctatattgat aatacagaat acactgttta caattatgct tatggcaaat gcccaaatgc
    26701 ttcgttaaaa tcaaaaatgc tattaagttt accactacat ttaaacttaa cagaaaacga
    26761 tttaaactta attgtatcaa ctttagaaaa agcattaaaa aattgcgtta aacccaatga
    26821 gagtcttatt tctgacttta gcaaatgata acatagccag tagccgaaca agggtattcc
    26881 aattcattcc gtatttaaat aaactgggca ttacttgtaa agttattgtc agtaacccat
    26941 attatgcaag acggtcaaaa aacatcttga aaaaggtcgc aaaaagtatt cacaaaaaac
    27001 taaataacct taatttcttt tttaccagcc tgttttttac taatattttt attcaaaaag
    27061 tatatttgcc cagatgggaa atcaagattt ttaaatattg tggtaaaaac attatttatg
    27121 attttgatga tgctatttat gtaaatgaac agagtcaggc tatttcaaaa aacaaacgca
    27181 aaaggcttga ttatgttttg aaacaagcag attggattac agttgaaaac gaaactaatg
    27241 caaactatgc caaaatttgt ggaaacaata attcaataat tatcaccggc ccaatagaat
    27301 gtaatcgtta ttttccacaa gaaaacacca attcgggcaa cgaatttgtt attggttgga
    27361 ttggaagccc cagtaccaca aaatatcttg aaattgtaaa aaggccactc caaatattat
    27421 cccaaacaca tcccaatttc aaactgatgc tgattggggc caatgatatt gcaatagaag
    27481 gggtaaatac tgaaaattac ccttggacat tagattctga agtagaattg ctaagaaaat
    27541 ttgatattgg gataatgcca ctgtataatg atatttggtg tgcaggaaaa ggcggatata
    27601 aacttttaca atacatggcc ttgggtatcc cttctattgc atcccctgta ggagtcaacg
    27661 atgatattct taatatggga gatattgggt tcaaagccat tacagaagaa gattggatca
    27721 aatcttttga aaagctaatc aacaataaag aaatacgctt gcaaatgggc aaaaaagcaa
    27781 gagaaactgc gttgacatat ttttcctacg aaaaatatgt ccatgattta attcattgct
    27841 ttagataatt catttttgag ttttctatta cttttctttc tttgagaacc aatctccatg
    27901 caaaaaaacc tttttatttc tataaccatc aacaggcaat attttgccga actatatgat
    27961 ttggatgttt taatcctccc gttataaacg ggacaagctg atttagaaat tgtttgaaaa
    28021 attttattaa attattgaat taccatattt aaatcgatta agcattttta atgcggaatt
    28081 ttgattttat aaacattcag acagaataaa taatgagtta atttttaaaa ttccaaagaa
    28141 gccataactt gcaacagaac atttcgttat ctttgtattg ctgattaaac attcattttg
    28201 ggttattttc aattttgttt tttgaaccaa attcatgtaa catcagcata gtccattgta
    28261 atttacaaaa gtcttttgta ctttttataa atacaatagg gataattttg cgaagaaaaa
    28321 atgaatacac aaattacaca attgtttggc atacaatatc ctattgtaca gggaggtatg
    28381 gtttggtgca gcggatggga gctcgcttca gccgtaagca atgccggagg tttggggctt
    28441 attggctctg gttctatgca gcctgaaata ttgcgtgagc atattcaaaa aaccaaggct
    28501 gctaccagca agccttttgg ggtaaatgta ccactgctat acagccaaat agagaaaacc
    28561 atggatgtca tcatggaaga aggagtaaag attgtattca cctcggccgg caaccccgca
    28621 acatgggcac aaaagctcaa agacaaagga ataaaagtag tacatgtagt atctaatgta
    28681 aaatttgccc aaaaatgcca ggatacaggc gttgatgcta tcgttgcaga aggggttgaa
    28741 gccggaggcc acaatggcag agaagaaacc actaccctgg tgctgatacc actgataaaa
    28801 aagcatgtta atatacctgt tattgctgcc ggcggaatag ctacaggcca agctatggca
    28861 gcagcttttg cccttggggc agaaggagtg cagatgggca cacgctttgc tgccagcata
    28921 gaatgttctg cccacgaggc attcaaacaa caaattgttg cattatcaga cggaggcaca
    28981 tatcttgctt tgcgcaaaaa agcccctgta aggcttatcc gcaacgctta tgccaatcgc
    29041 atagaggaag ctgaaaacag cgggcaaagt ttggaacagg ttttgcagct tgtaggcaaa
    29101 ggacgtagca aactgggtat gtttgaaggc gagctgaatg aaggcgagct ggaaatagga
    29161 caagcagcag ccttgattga tgctgtacaa ccctgtgatg tgattatgca gaacattatc
    29221 agtgagtata accaagctgt aaaagggctg aaaatactgg actaaaattt taataaccca
    29281 attgtatttt gcacgtaaac ttctcatata aaaattgaaa ttatgaagaa tatcattgca
    29341 acaatcatca tactttttac ttctttatca ggttcattaa tggctaaaga tccaattcct
    29401 tcgccagaaa aaatacgcat tacaaatggc agtattttag gaaataatga agacgcaaat
    29461 tattatacaa tccgatggaa ttataataac ctaaatttag tggattatta cgtgatatat
    29521 tctgaagata aagtttctcc taatggatgg aaagaagaag gaaaacaacc aataaataca
    29581 aaaaaagaat ttccattaaa ctcatttaat ggagagcaaa tcagtcaata tactgttgaa
    29641 gctgttgcaa aagatcaggc aaattatgac gatagtaaaa caaatatgac taacagctat
    29701 acagcccaaa atattgtttt aggcactgcc tataatgctt gtaataatga attgactttt
    29761 acttggaata gcaatcctct tgtaacacca aggagtcaag ttataagata cactattgat
    29821 ggcgtttatg atgaaaaaaa atatttttca gttacaaata ctgaaaacaa atatattctt
    29881 gcaactaata aattaacact aaaaaaagga gtacaataca aatttcagct tgaaattctg
    29941 tacaaggatg ttgaagatta tacatactca aatattattt cttttacaat accagaaacc
    30001 gccaaaacat ttccagatta tatcaacatt gacaagctgg atgccagcag cgggaaagtt
    30061 gtactaaaca taaatattga ccctaaaaac gagctaacag acttatgggt ttttaggtca
    30121 ttatcaaccg aacaaacaaa gtcagatatt ataaaaatca agcttggttc tgctgaatat
    30181 cccagcctaa acaatgtatc aataacagat gattcagaac ttgaattaaa aacaagccaa
    30241 tataaataca ctgtatcatt acttagctgc gaaaatgttg taaaaagcag tagtatcttc
    30301 acgctgccat tagaagaccc gcaagaacgt gaaggttctt tagacctttg gtggaaacct
    30361 attcagcctt ccacccaatt agaccattat gaaattacaa ggtatagaaa caatggctca
    30421 tctacactgg aaacgcctgc aaatactgct attaatcaaa gagactactt aaacaaaata
    30481 aaagataaac aacccgggga attcatttgc tataccatta ccgcatatat gaatgtgaac
    30541 tttgggactt cagtaagcaa taaggtatgc tatcaaatag acgaaatggt aactatgccc
    30601 aaatacatag ttattggcga aaataacaaa gccatttctg actttacctt tccttccaat
    30661 tcttataccc ccgaaaaatt taccatgcgt atttacgatc ggcttggtac aaaattattt
    30721 gaaacaacag acccccaaaa aggatggaac gggtgctata acaatggcaa tgggagccct
    30781 gtaccggcag gggcttatat atactccatt gattttacag gcaatgacgg aaaaataaaa
    30841 catcagcaga actcatttat tgttgtacac aaataagcta tctttaacca tatatgagca
    30901 aactgaccga tgaagaaata aaagaaataa acgagctata caggcttctt attaccaata
    30961 gccccagatg taaagaggac gaagaaaaag cattggttga taaagctttt cgcatagcct
    31021 ttgatgcaca caaagatatg cgccgtaggt caggcgagcc atacatcata catccacttt
    31081 tagtagcccg tattgtttct gaagacatcg gactgggagt taaatctatc atcagcgcat
    31141 tacttcacga tgtagtagaa gataccgaaa ttacccttga agaaatagaa cacacctttg
    31201 gccccaagat tgcttatatc attgatggac taaccaaaac caaagagata gaaaaaaact
    31261 cgattatccc cgacctcatt ggcgaagtat cagaagaagt gcggtcaaaa cagttggaca
    31321 attttaaaaa gatgctactt accttatctg acgatgtaag ggttatcttt ataaagctgg
    31381 ccgacaggct gcacaacatg cgtacgctaa gttcaatgcc gcgcgacaaa cagctcaaga
    31441 ttgccagcga aaccatatac ctttacgccc ctttagccca tcgacttgga ttatactcca
    31501 tcaaaactga gctggaagat ctgagtttgc gttaccgcaa ccctgaaata tacaacgacc
    31561 ttcagcaaaa acgcgaagag gcagtaaaac aacaagacga atatatcaaa aacttcattg
    31621 acccaatcag taaaaaactc aaagacaacc atattgaatt tgagatatca agcaggttca
    31681 aatcggtata ttccatctgg aacaaaatcc agaacaagaa agtatctttt gaccagatat
    31741 acgacatttt tgccattcgc ataatatttg cccccagcgc tgatatatct gaaaaaacac
    31801 aatgctggaa catttactct atcattagcg acttgtacat gcccatgcca gaaaggctgc
    31861 gcgaccatat caccaaacca aaatctaatg gatacgaggc gttacacatt acagtaatgg
    31921 ggccacaagg ccgatggata gaagtgcaga tacgcaccaa acgaatggac gaaattgccg
    31981 aacgaggcct ggcatcgcat ttcaaatata aaaacgacaa ccagcaggaa agcgagctgg
    32041 acaagtggct acgcgacatc cgcgaaatgt tgaaaaaccc tgattcaaat gcactggagt
    32101 ttattgaaga attcaaactc aacctttttt cgtcagaaat acaggttttt gaccgaagcg
    32161 gcaaattagt aaccctgccc aaaggttcta cggtacttga ctttgcgtac tatatccact
    32221 ccaaaaacgg taatactgca atgggcgcta aggtaaacca taagctggtg cctatcaacc
    32281 atgtactttc aagtggcgac cacgtagaaa tcatcaactc ggataaaaac agggttcaac
    32341 gcgaatggct cgactttata gtcacagcaa gagcaaaatc ggccatcaaa aacgtgatga
    32401 aagccgaaaa tcgcaacagg cttgaaaaag gcaaaaaaat gcttgaatca gcttttaaac
    32461 aatggggaca atcgctcaac tcgaaaagcc tgcgaactat cctcgaagag tttaggatga
    32521 gcaataaaga agaactttat agcaagattg gcagtgaaat catcctgttg gatgacatcc
    32581 acaagatttt taaaaaagaa tcgtccaaca aatggatgaa atactggaag ctcaccattg
    32641 gacgagctac aggaataagc cttggttcat cagacaaacc ccacccccct gtaaaaaaaa
    32701 gtaataaatt ttctaaagac aatcctttta tccttacgga gaatattgaa gacaacaccc
    32761 gctcgtatat cattgcccca tgctgtacac ctcttcccgg cgaagaagta attgggttcc
    32821 ttaatgtacg ggacgaagta atcatccata aatcaaaatg ccctaatgct gtaaaattgc
    32881 tggccagcca aagcaacaag attgtcccta cccgttggac aacccacaaa atactatctt
    32941 tccctggcaa gatacacata aaaggcatag acaggcttgg tatctacaat gatattattt
    33001 ctaccatctc aaaagacctc aacatcaaca tacgttctat caacatgtcg agcaacaacg
    33061 gcatttttga aggagagata gagctctatg tccatagtat caacgatatt aataaactta
    33121 ctgttaccat caataaaatc aaaggggttg actttgtcaa acgtgttgag aacattgaac
    33181 aataggtaaa atgggattgc actttcgtaa attgggtcaa gggcagccac ttatcatctt
    33241 gcacggccta tatggcagtt cggacaactg gtatagcatt gccaaggtat tttcagctta
    33301 ttttacagta tataccatag acttacgtaa tcatgggcac tctgctcatt atcctacaca
    33361 tacatacaac gacatggccg ctgacatagt taatttttgt caggagcata acattgaaaa
    33421 agcaacattt gcagggcatt ctatgggagg aaaaacagca atgttacttg cattggagca
    33481 tccacaatta attgagaaac tcattgttgt agatattgcc cctaaagcat actcgttgct
    33541 attagagcca tcagagcatg tattgcagca tattaacatc atgcaggctt ttgcaaatgt
    33601 agatttgaca ggacttacaa aacgagaaga agtggatatg cttttagctg aatacatcgc
    33661 cgacccccct acccgacaat ttttaatgaa gaatcttgtt agaaaagaaa gtggttttca
    33721 atggcagtta aatacaaaag ttttaagaaa ctcgctacct gaaataatta atgggcctga
    33781 ctttaacaaa atattatttg acaaaagcca aattacttac ccggttttgt ttgtcaaagg
    33841 agaaaaatcc gggtatatca aagacgaaga tatgattact attcgtaaaa ttttcccaaa
    33901 agcagaatta tctgtcatat tcaatgccgg gcactggcta catgccgagc aacctcaaag
    33961 ttttattaac accctcaaat attttctttg tctatgaaaa ttttcgataa gcttgccgat
    34021 aaaataacat tacgcaaagc caatgcatta gaacttattc ctgtaaagct tgtcaatgca
    34081 aaagatgaaa acatcatgcc tataactttg ctcgtcccaa ggttcaacaa tcggtttctg
    34141 acgggcatta tcaggcgccc tgaacacttc accgttcgcc ttgacgagac cggcagcaaa
    34201 gtatggcttt tgatagatgg taaacgcaat gtttttgaaa taacacaaca cctctgcgct
    34261 cccaatgagc catcagacga atggttccga cgcgtagcag aatttgttac aagcctttat
    34321 aaaaaggatt tggtgaaact ggaggttaag aaacagtttt attaatttga gttgtaattt
    34381 ttaaccacaa agaaaacagg gttttacaac gagctgtaaa gaaaaaaagc attgtaacac
    34441 tttgcttaag atagctttgc agcatcttca gttaattatt acatattaca caaactcccc
    34501 atccggatac aaaaaattgt cgtaaggata acgagtgata tgtatttctt ttactttgtc
    34561 ataaagggtt ttgcgaaggt tctcaatatt ggttttattt tttgccgaaa taaatataca
    34621 aggagcatta acactggcaa tataactatt gttcaattct tcgacacaaa gcgggttatc
    34681 aggcatgtat gcatcaatct tgttaaacac tacataagtc aacttattag cagcccccag
    34741 ctctttaagc gtttgattga ccacagctaa ttggtcctca taagaaggat gagaaatatc
    34801 tactacatgt aacaaaatat cagcttcacg cacttcgtcc aacgtagatt taaaagactc
    34861 gacaaggtga tggggcaatt ttctaataaa accaacagta tcagacaaca aaaatggcaa
    34921 attgccaaca accaccttac gtacagttgt atcaagtgta gcaaacagct tattttcggc
    34981 aaatacatcg cttttgctca aaaggttcat taaggttgat ttacccacgt tggtataacc
    35041 tactaaacat acccgtacca tctgaccacg atttttgcgc tgagtagcca tttgggtatc
    35101 aatctttttg agctgttctt taagtcgggc aatacggtcg cgaatcacgc ggcggtcagt
    35161 ttcaatctcg gtttcacccg ggccacgcaa accaataccc ccacgctgcc gttcgaggtg
    35221 agtccacatc ccaacaagcc gtggcagcaa gtattgatat tgagcaagct ctacctgagt
    35281 tttggcatgc gaagtacggg cacgtttggc aaatatatca agaataaggt tagtgcggtc
    35341 aagcacctta cagttggttt ttttttcaat atttttaagc tgtgaagggg taagctcatc
    35401 atcaaaaata accagagtaa tttcattttc tttaataaat tcggctattt ctaaaagctt
    35461 tccttctcca acaaaagtgc ggggattggc aacatcgagc cgctgaaaaa atttttttac
    35521 taccacagct ccggcagtat ctgccaaaaa ctcaagttca tcaatatatt ccctggctgt
    35581 ggcttcatct tgttgtgaag aaataagtcc aaccaataca gcagtttcag caggaggagc
    35641 aagatttatc atgcaatatt ttttggtaaa aataagccga gtttaacaga taatcaagaa
    35701 ttaaaaaagc ctcaaaacaa gcactatgta actcaaaaaa tcaaaatatc ttctacgtta
    35761 attatcaaac acttacaata aaaaaacaac cattattaag tagcaatgaa gaataaaaat
    35821 actatcttag taaaaatttt ttatcatggg cgtattacaa tctttagttt tgaaaaacag
    35881 aagttacagg cgtttcaaag aagatgaaaa catttcttcc gattttttaa aagaattagt
    35941 aagcctggcc agcattaccc cctccggacg caatgcccag ccactaaaat atgtactgat
    36001 taataccccc gaaacaaacc taaaggtatt taacactttg tcttgggctg cataccttaa
    36061 aggtaaaggg gctccgaatg aaggcgaaag gccttcggcc tatattgtaa tacttttaga
    36121 caagcacatc tccgaaaacc ctttttgtga tcacggtatt gtagcacaaa ccatgctatt
    36181 gggtgcagta gaaaaaggtt atggtggttg tatgattatg aatattaaaa aagaagagct
    36241 aagcaatata cttaaccttc cagaacactt tgaaatactt atggtcatag cccttgggaa
    36301 acctatagaa caagtagttt tagaaacaat gccccaagat ggtaatttcc aatactggcg
    36361 tgacgaacag caagtacacc atgtcccaaa acgaagcgtc aatgaactga tattgaaaat
    36421 acaataaata cactatcaaa tgaaaatcaa ccaacttgtt ttaaggggaa tagcgttgct
    36481 tcttcttatc atagcagcta caagcttgat aaacagtcag acaggcctgc cttatatttc
    36541 taattttact ataagtgaag aggatcttac tgaaaattat tctttggttc aagattcgca
    36601 ggggattata tttactgcca accgcaaagg aattttatct tttgacggag aggactggca
    36661 aattattaaa actccggata tgccaactgt tttggcaata tcttcagcta caaataccat
    36721 ttatgttgga tgccgcaata ctgttggtta ccttagcaag aactcattgg gcaaatatga
    36781 gtttaacagt ttgtctgacc ataaaacagg gtttgtcagc caaatcatag ttcataacaa
    36841 caatgtttac tttctaagtg attctctcct gctttatgtt acagataata gcttgccttc
    36901 aaaagtagtt gttgccaaca accaggccaa taaattcatg tgcatgatac cctttggttc
    36961 gcaattattt atagatattg caggaaaagg gttatttagt ctaaaaaaca acaaagcaga
    37021 tgagaaggtc aagaatttta cgctaagcga acaggttgta tttaccatac cccttgaaaa
    37081 aagcacaata ataggggctt cggacaacaa actctacttt tttgatggaa aaaacgtcag
    37141 cgcttttcgc tctcaggctg accaaatcct tttgcaaggc acaattattg gaggtaagaa
    37201 ggtaacccaa tcggtaatag ccattgctac tgcatatgca ggatgtattt ttattgacat
    37261 caaaacgggc aaaaccatct ataccatcaa tactcaaaat ggtctgccag acgatgccat
    37321 ttatgccatt gaagttgacc aaaacaatgg gatctggctt actcatggat acgggcttag
    37381 ccgcattgac attaaccttc ctgtcagaaa cttcagccat tacaaaggac tcgacggaaa
    37441 actacatgcc attgccatgt ttaaaagtaa gctttatatt ggcagtacaa caggagtgct
    37501 tgttttaaac gaaaaaaaaa tatttgacgt ttcaacccgc caacaaacaa taaaacctat
    37561 tcagccggca gcatcagggt gggcatctga tcaacctcag gtttcagcag aaaaaagagg
    37621 cttttggtca aaactattta ccaaaaaaat aaaccccgaa aatgaaaaac caaccaacaa
    37681 acaacctttt gcccgttcag aaaaaagcat taaaatattc aaacaaatag ccgagcgcta
    37741 tgaatacgag aaggtgcaag ggataaactc gcggtgcaaa cagctaatcg tttacagcaa
    37801 tcacctgttg gcttatacaa caaatgggct ttatgaaata tcaggcaata gtgcaacaca
    37861 aatattgagc ggcaacgata tcaattatct tatccccgta aatgacgaca aagcactata
    37921 tgtttgtacc gaaaaaggca ttaaaatttt gaaagatgaa aatggtaagc ttatttcttc
    37981 agattttgaa attatgcaaa accagcccgt atattctctt gcaaaagaca tgttcgacaa
    38041 ttactggttt ggattggaaa ataaggttat taagataaaa gtcaaaaatg gaaaaataaa
    38101 agaaaaaaag gcctataatt ttaacagtga ttttcgcgaa agaataattg tccgcatttc
    38161 taataaaaaa cctgtattct ttttatctga cggcgtttat tccattttta acgattctat
    38221 tcaacctaac ctttctttat caaaatatat caacaaagat tcgaaagtct attttcctgc
    38281 aggaaaccac acatacatca atagtaacaa ccaatggata aaaattgagg aaaatagaga
    38341 ggttgacagt acctcatacc aatatctggg cttattttcg aatataaatc aaatttgcag
    38401 cgacaaagac agcaatttct gggtgataga cgaaagcacc aacgtgtaca tgctgaatgc
    38461 taaagagatt gaaaaatata aaactgactt taaggctttt gtgaaaaaat tctttagtaa
    38521 cctttcaggc caggattttg cattaaataa tgtaacattg gaaagaaaag acaattacct
    38581 gaaaattaaa atcggggctc catactacat caagaaaggt acaaatcaat accaaatcta
    38641 tatagaaaaa ctaatgccag gatggagtgc atggagcaac aactcagaat ttgacattcc
    38701 tttatcgcca ggcaaatggc aaatacaagt tcgtgccaag aacatccttg gagaaatatc
    38761 tgaaccccgc actatttctt ttaatattaa aaaaccattt tataacagct ggtggtttta
    38821 tatgctttgc atagcagcat tggtaggagt tatttacctc attattaaat tccgcgagcg
    38881 ccagttacaa cacgagaaag aagtgctgga agaaaaagtc cgcgagcgaa cccgcgaaat
    38941 tgaaaaacaa aaagaagaaa tcgaacttca acgaaataaa attataaaaa tcaacaacga
    39001 aattatcgat agtatccgct atgcaaaacg catacaagca gcagcattgc catctgatta
    39061 tattgaacct tcatacaaat tgaagacttt tgtctattac aagcctaaag acattgtttc
    39121 aggtgacttt tactggataa aacaaaagaa tgacattgcc atgatagctg cagcagactg
    39181 cacagggcac ggagtgccag gagggtttct cagcatgctt ggaatatcat ttctcaatga
    39241 actggcagct atagagcaaa aacttactgc tgctgaatat ttaaatatgc tccgcgtcaa
    39301 ggtaaaaaca accctttcat caaaaatgga agatatagaa agcaaagatg gtatggacat
    39361 tgctttttgt attgttgatg taaaaaataa aaagttgcaa tatgccgggg caaacagccc
    39421 aatctatctt gtccgacaaa acgagcttat tcaatacgat gctgacaaaa tgcccattgg
    39481 ctctttcatt gccgaaaaag actcatttac caaccacgat atcttgctac aagacggaga
    39541 tcgggtatat cttttttctg atggttaccg tgaccagatg ggtggcccta tgcaaaaacg
    39601 tttaaaaacg cctggattca aacagcttat tttacaagct tctcaaatgc caatagttga
    39661 acaacataag cattttgaaa gtttttttga aaaatggaaa ggcgacaacg aacaaatgga
    39721 cgacatactg gtaatggggt ttggaatata ataatatcta aaacttagct ttgccgcaac
    39781 gaagctgcgg caaaatgaga tgttgccgag aaattaacaa atatttcaaa ctaattcatt
    39841 ttgcttctat tctgaaattg tcaatccatt attattaact aaacctgtta aaagatttca
    39901 tttcattcat cttgtcagcg gtttaataac ttgtactttt atgggtctaa ttcaaaaagc
    39961 aaaaaagccg tccccaacga gacggctttt aatatatcgt ttcaagaatt atttctcaat
    40021 acgcattttg taaaacgaac gccatacaaa aacaagggca actgctataa atatcaaacc
    40081 aacataaggg gcagcagcac ggaaattttc gctaatagca atttccatag ccaacaaacc
    40141 aaacaaagtg gtaaacttga taatagggtt catagctaca gaagaagtat ctttaaatgg
    40201 gtctcctact gtgtcgccta caaccgtagc agcatgtaat tcggtacctt ttgccttaag
    40261 gtcaacttcc actacttttt tagcattgtc ccatgctcca ccagcgttgg ccataaatac
    40321 agcctggaat aaaccaaata cagcaattgc aataagataa ctaacaaaca atgaaacagc
    40381 agcaacgcca ccaacacttt caggtgatga taaacaagca atagctaatg caaagaagaa
    40441 tacagaaatg aagatgttga acattccttt ttgagcatat tgtgtacaaa tcttaactac
    40501 ctcagaagaa gatttagcat cagctttttt aggggcatta ggatcaaggt tgatgtgttc
    40561 tttgatgtaa gccacggcgc ggaatgcacc tgttgatacg gcttgagttg aagccccggt
    40621 aaaccagtaa ataacagcac caccaaggat aaagccaaga attgtatatg ggttcaacaa
    40681 gttcaaaata gtttcaggct gaacccctaa agttttttgt atcattaata tcaaagagaa
    40741 gatcatggtt gtggcgccta ctacggcagt cccaataaga acaggtttag cagtagcttt
    40801 aaatgtatta ccggcaccat cgttagcttc aagataatgt ttagattttt caaagtcggg
    40861 tttgaaacca aaatcctttt caatttcagc tccaacgttt ggaacctctt caatcaatga
    40921 aagctcatat acagactgtg cattatcggt tacaggccca tagctgtcaa cagcaatagt
    40981 tacagggccc atgccaagta aaccaaatgc tacaaggcca aaagcaaaaa ttgacggata
    41041 aaccataatt tcgctcaatc cttttgtgct cataaaatat gctatgaaca tcaatacaaa
    41101 aaacactacc cctttccaga aagctgaaaa gttaccggca ataaaaccag aaagtatggt
    41161 caatgaagcc ccaccttctt taccagactc aaccacttct tgtacgtgtg ctgattttgg
    41221 tgaagtaaat attttggtaa actcaggaat aagtgcacct ccaatagtac cacagctgat
    41281 aattgtagaa agtatccacc acatattggt gttaccattc aaatcgggaa tcatcacata
    41341 actggcagca aatgttacaa taattgacaa aattgaggta atccatacaa ggtttgtaag
    41401 tggttgttcg aaatcgagat catcgacatt tccgtatttt gcattactta acatgccgtt
    41461 gatatagtaa gaaacaattg aagtaatgat catcaaaata cgcataacga aaatccaagt
    41521 cagcagctta accattaaac ctgaagtaac aatttcgtcc ataccaagag ccgaaccacc
    41581 aacagccaga aggatgaaag agataagggc aacaccggtt acaccgtaag tttcaaaacc
    41641 atcagcagta ggaccaacac tatcgccggc attgtcaccg gtacagtcag caataacacc
    41701 aggattacgt gggtcgtctt cgccaatttt gaaaactact ttcatcaggt cagaaccgat
    41761 atcggcaatt ttggtaaaga taccaccggc aatacgaaga gcagaagctc cgagagattc
    41821 accaatagca aaaccaataa agcaagcacc agccaaatga ccaggcataa gtaaaagaat
    41881 gataagcatc atgataagct caatacaaat aagcataaca ccaatgctca tacctgcagt
    41941 aagaggtata ttaagaagtt tcaggggctt cttttctaat gaagcaaaag ccatgcgggc
    42001 gtttgccaaa gtattcatac ggataccaaa agcagctacg ctatatgaac ccaaaatacc
    42061 aataatagtc caggcaagga taagcaatac acctccaaaa ccaaacagcg aatggccttc
    42121 atggtctttg gccaaaaaac caaaataacc tgcaacagca gcgccaatga aaatgaaaag
    42181 aatggctaaa aacttacctt gttgcttaag gtaggtcgaa caagttttgt aaatcacttc
    42241 agacacgtcg agcatagcct gatgtgcagg aagtttttta actttcataa actgataaag
    42301 gccaaaaagc aaaccaagtg cgcaaacaac gaatccggca tacaaaatac cttcactttt
    42361 tatagcttcg ggtactttca aatcagcttc actcgccatt gccagcatag gcattgccat
    42421 agctgtcaaa attgatagta actttttcat aatgaactgt ttgtattagt gtgaataatt
    42481 ataatttttt taggacacac aaatttataa atagttgtaa catattaaac taaatcggtt
    42541 ataaatttta ctgtagaacc caataaattc aatatttatt taacttgctg attttgaact
    42601 aatcgtaaat acaccaatcg gaacatcata tcactgttta taccgttcaa cccctttttt
    42661 acgccattca ccgaaaaaca atttcacaat ttacaactta cctttttttt tgcagctaaa
    42721 tacaaaatat acgcagaaaa cttttaacat atttttagtt tccggtgttt atttagaaat
    42781 ttacttttgc gccaaatttt tgacttgatg aatgaagagc taaaacagat tattgagaaa
    42841 gtacaagcgc tgtatttcaa gtatggcatc aaaagtgtga ctatggacga tgtggcccgc
    42901 gaacttggca tgtcgaaaaa gaccctgtac cagcacgtca gcgacaaaaa tgatttggta
    42961 caaaaggtgg taaactatgt catggaacga gaccatgatg aatttgacga aatttttgaa
    43021 aaccacaaaa atgctattga agagcttatt gaaatgacgc tttacattaa taaagtgatg
    43081 aaaaaccaca atccttcaac catatatgac ctgaaaaagt actatcccaa cttatacaag
    43141 gtaatgatgg aaaaacggcg tgacaaaatg tttcaacaca ccattaataa cctgaaaaaa
    43201 ggaaaagaac aagggatata tcgtaccgaa ataaaagaag atataattgc taaatatacc
    43261 accatattaa cctttcaaac ctttgaaaat gacactcttg acatgaacga atttacttca
    43321 ccagactttt tcaacgaagt attcagttac catattagag gtattgcaaa tgcaaaaggt
    43381 cttgaaatat tggaacataa cttgaataaa attacagaaa acaagtaaca agataaatag
    43441 atatatatga aacgagcatg tttagatgca atcaaaaact cgaatgaatc ccgattagtc
    43501 gggacgagtt ctatccgaca tttaaaaaat aaattagttc ggataaaaga aaagaaatta
    43561 ttattaagtg tgcttttcat ctgtctgacc ttgcagacct ttgcctcagg cggtgcagac
    43621 agctcaatgc agttttcgtt ggagcaggcc aagcaatatg ctctggaaaa cagccctctt
    43681 gtaaaaagtg ctgcattaga tcttgaagct gccaaaaaga aaatttggga aaccacagct
    43741 atagggcttc cgcaggccag tgcaaaatat tcatacagtt atatcctgac attgccagag
    43801 atatacaagc aatttgcaca aatgggaggt ggtggcgaag caaacctcga tgatatgaaa
    43861 tcctcatcta cgcttgatat cactgtcagt caactgcttt ttagcggtgc atatcttgtt
    43921 ggattgcaaa catcaagggt ttacaaaggt ttgtccgagc tatcccttac taaatcaaaa
    43981 aatgacctta acgaatcagt taccaacgcg tatttattgg ttctcatagc taaagaaaat
    44041 aaaaaaatac tggatacaat gtacaacaat atgaatgcct tgcttgaaac tattaaagca
    44101 atgaattcaa aaggatttgt agaagaaaca gatgtagacc aatttcaaat tacagtaaac
    44161 acagtaaaaa acaaccttga gttagtaacc aatcaggtat tgattgccga aagaatgctc
    44221 aaatatcaac ttggctatcc gtttgaatct gctattacgt tgaccgacag catcaatgga
    44281 tttgtaaaca acatcaacat caatactata ctggcagaac cttttcaggt tgaaaacaat
    44341 gttgattaca agttagttga aacccaaaca aagctgatga aattgaacct aaaattgaac
    44401 aaatcagagt ttttgccaga cattgcagct ttttatcaac atgacaaaaa cttcaacgat
    44461 aaagccgttt cgtttactcc tcccgattta gtcggattaa caataaacat tccgattttt
    44521 agcagtggac aacgtttggc caaagttagc caggcccgta tctcttacga aaaagccaaa
    44581 atatcgcaag accagacagc caaaggttta aaaatacagt ttgaagacaa caaagccaag
    44641 tacatcaatg ctttgggtaa ttatgaatcg tcaaaaagca actacgcttt agccgaaaaa
    44701 atatacaatc ggtcaataac caaatacaaa gccgggatga tctcaagcag cgatttgacg
    44761 caagtacaaa accaatattt acaggcacaa acaaactatt tcagtgctat actggattta
    44821 acctcggcca agtcaaagat tgaaaacctt atttcaacag taaaataaaa ttttaaaaaa
    44881 taaatatcat gaagaccaaa ttattaagtt tagcattcat cctcgttttg gtttcttgtg
    44941 gcaaaacaga taaaaaggct gaaatagcca agctcagaaa gcaacaagct gagctggccg
    45001 agcaaattaa gagactcgaa gctgaagtag caggagattc aaccaatgta gccaaaggaa
    45061 cacgtgtagg cattaccgat gttgcatacc aggactttat ccactgtatc gagatacaag
    45121 gcaaagtaga tggcgatgaa agtgttgatg tatttgccga aggcactggt ggcattatca
    45181 agcaaatact ggtaaaagaa gggcaatatg ttaccaaagg ccaggttttg gccactattg
    45241 acgacaaaat ataccaggtt cagctcaaat caaacgaggc aaacctcgaa tatgtcaact
    45301 ccctttatga aaaacaaaaa gcattgtggg accaaaagat tggcagcgaa gttcaatacc
    45361 tcgatattaa aaacaaaaaa gaaagccttg aaagtgctgt tgaaaccatt aaagaacaaa
    45421 tcaacatgtg caaaatcaaa gcccctatta atggaactgt tgaaggtata acaataaaaa
    45481 ttggtcaact ggtaggcccc gcaatacctg ctttcagggt tgtaaacctc tctacattga
    45541 aaattacagc agaagtggcc gaagggtatt caagtaaagt ccgaaaaggg gacaaagtat
    45601 caatttattt gcctgacatg aaaaaagaat ttaacggcaa tgttgacttt gacagcaaat
    45661 acatcagccc tgtcaacagg actttcactg tcaatgtgcg tatcccttca aatgacaaag
    45721 acataaaagc aaacatggta gctgttgtcc gtattactga ttatcacaac cctaaagcta
    45781 ttgttgtccc attaaacgtt gtacaaagcg acctcaacgg ccagtatgtt tttgtagcac
    45841 aaaataaaac tgcaaaaaaa gtacatatta ccacaggtga aatttatgcc gggaacattg
    45901 aaataaaaag cgggctaaac ataggagata aggttattac tgtgggatac caggaattgg
    45961 aagacggaca ggctatcaat ttttaagccc atgtttttgt aaaatataaa ttgacaaata
    46021 aagccttaac ccaggccaat aaccaggcaa gacattaaaa atttttatat ggaaaaacta
    46081 aaattcaaag agtttaagcc aacaagctgg agtattgata ataaaaccag tatttatgtg
    46141 cttaccttta ttatcgcggt atttggtttg ataaactata ataccattcc taaagaacaa
    46201 ttccccgaaa ttgtaattcc acaaatcatt gtaagtacag tttacccggg cacctctccg
    46261 gcagatatag agaaccttat caccaggccg ctggaaaaga acatgaagtc aataaacggg
    46321 gtaaaaaaaa ttaccagtaa atctgtacaa gactattctc ttatcattgc agagtttaac
    46381 acagatgtaa aagttgccga tgccaagcaa aaagtaaaag acgcagtgga caaaacaaaa
    46441 aaggacttac ccagcaacct gttggaagac ccttttgtgc aagaaataga cttgtcagat
    46501 atccctatca tgtacctcaa catctcgggc aattacgacc tggccaagct gaaaaaattt
    46561 gctgaagacc ttcaggaccg cattgaatcg ttcccggaaa taacccgcgt ggacattgta
    46621 ggagcacttg acagagaaat ccagataaat gttgatatgt ataaaatgca agctgcaaaa
    46681 attacatttt atcaaataac tcaagctata ggcaacgaaa acatgactat tcccggaggt
    46741 accatcaaca tgcaaaacat gacccgcagt atcagggttg taggtgagta taaaaatata
    46801 gaccaaatca aaaatactgt acttatttca aatagcggcg ctattgtcta tttaaaagac
    46861 atagccgaga taaaagacgg ttttaaagac caggaaagct attcgcgttt caatggcaaa
    46921 aatgtcatta cgctcaatgt aatcaaaaaa agtggaaaaa acttactcga cgcttctgat
    46981 aaaatagaag cggccattgc cgaatttaag gaaaacaaat ttccgtcaga cctcgaagtc
    47041 agcatcaccg gaaaccaggc aaaattcaca cgcagtatcc ttaccgagct caacaacacc
    47101 attatcatag gttttatact ggtcaccatt gtgctcatgt ttttcatggg tgttacaaac
    47161 gctatatttg tgggcttgtc agtaccattg tcgatggctt tggcctatat tttcatgccc
    47221 tggatagact ttaccatgaa catgctggta atgttttcgt tcatctttgc ccttggcatt
    47281 gtggttgacg atgctattgt ggttatcgaa aacacacatc ggatatttaa gaaagagaat
    47341 ggccggatga ctattaaaga agctgccaaa aacgcagccg gcgaggtgtt tttaccagta
    47401 ttgtccggaa cattgaccac cctggcccca tttttcccat tagctttttg gccgggagta
    47461 gtagggtctt tcatgttcta cataccggta actattatta ttactttgtt tgcttcacta
    47521 ttagtggcat atattatcaa ccccgtgttt gcggttgact ttatgaaaca tgacgaagac
    47581 cacactgtaa aactacacca aaagaaggcc tacaaaaacg gagcaattat tattgccatt
    47641 gcagctttct tctacatcgt aggtggcttc aaatatggtt caaccatgtt tggcataggt
    47701 acattccttg tcctggttgc catattgtac gtagggcaca atactttctg gtacaaaata
    47761 atccataagt tccaaaacag ggtaattccc ggattaatgt cacgttacga ccgcatcctg
    47821 cgctgggcta ttgaaggcaa aagaccttac aaactgctat gggggacatt tattctacta
    47881 attgtttctt tcattttaac agcaatagca gggccaaaag ttgtattttt ccctgaagaa
    47941 accccaaaaa ccattatggt ttatgccaaa ttgccagttg ggacaagggt tgaatatacc
    48001 gactcgattg ctaaagagat tgaaaaacgt gttaacactg ttcttggagc aacaaacccc
    48061 gatgttgaat caatagtaac aaacgtagct tttggcgcat ccgatgacat gtttgatgga
    48121 ggcattgtaa catccaacaa agccaagata gctatcaact ttgtcgaata caacaagcgt
    48181 acagtgaatt acaacaccat ggtttatatg aacccgctgc gtgaagccat gaaagatatt
    48241 gccggcgctg aaattaccgt tgacaaaaac caggatggcc ctcctaccgg aaaacctatc
    48301 aacattgaaa tctcggctga agaccttgac gagttgatta ctacaacaga caggtttata
    48361 cgctttctcg attcaatcaa tatcccgggt attgaaaaat ataaaactga ttttcaaaaa
    48421 actaaacccg aaattattgt tgacattgac cgtttacggg ctaaccatga aggtatatca
    48481 tctgcgcagg taggctcagc gataaataca gcagttctag gaactgaaac gtctaaattc
    48541 agggatggag aagaccaata cccaatccaa ctccgcttta gcaaagcgca acgtgaagat
    48601 gttgacaagt tgataaatat ggaaatcacc taccgcgata tgaattccgg aatgctccgc
    48661 tctataccgc tttcatcgtt agcaattgta aaataccaga attcagttgg aggtattaac
    48721 cgtacagatt taaaacgagt tattactgtt acatctaacg ttctcgacgg gtacaatgcc
    48781 aatgaaattg tggcaaaaat tagaaaagcc attccagaat ttcaaaaatc tgataaaata
    48841 gacatcaagc ttaccggaga gcaggaagac caggcagaaa ctatggcttt cttgtcaaaa
    48901 gccatgatat tggcactttt cttaatattg tttatcctta ttacccagtt taactcattt
    48961 ggtaaaacag ccataatcct tagtgaagtt attttcagca taattggggt acttctcggc
    49021 tttaccatct gcaatatgac attctctatt atcatgacag gaatgggtgt tgttgcattg
    49081 gccggtattg ttgtccgaaa cggcatattg ctagttgaat ttactgatgt acttaaggaa
    49141 agaggattgc gtacacgcga agccattata caggcaggta aaacacgtat cactccggtt
    49201 atcctcaccg ctactgcagc tatattgggg ctgatacctt tagctattgg tttcaatatc
    49261 aactttgcaa cactattcac gcacctcaac ccccatattc attttggagg tgacaactca
    49321 atgttttttg gcccgttggc gtggacaatt atctttggcc tgattttcgc aacattcctc
    49381 acccttgtgc ttatcccggt aatgtatttc attacctatt caggaaaagt aaagcttgcc
    49441 cgtgcccgtc acagaagcaa gatagaaacc atgcgtgaac tggaagaaga tgaactgaat
    49501 gaatattaaa gctcaatatc tatagtacag aaatggcccc tcctaacctc cccttataaa
    49561 ggcaagggcc ctaacctaag ttcagggcag gaatcccctc ctttataagg aggggaaggg
    49621 gtggtaaaaa acaaaacctt tcaggaatta aaaaactgga aggttttatt tttacaaaaa
    49681 tctgtacttt tacacatcaa caattcataa aataaacgtc atgcaatacc aggttattga
    49741 ggtcaatgat aaaaagactg cgcgcgagtt ccttcttttt caggtaaaac tttacaaaaa
    49801 cgagaaatat tatatccgtc ctttagatac agatatcgaa aaagtatttg accccaagca
    49861 aaacaagctg ttcagacagg gcgaatgcca acgctggata ttgaaagatg aaaaaggcga
    49921 aactgtaggg cgtgttgctg cttttgttga ccgcaagagt gctgccaaga atgaccaacc
    49981 gacaggcggc atgggctttt tcgattgcat caacgacaaa acggccgctt ttgctttatt
    50041 tgacacctgc aaaaaatggt tagaaacccg tggcatggaa gctatggacg gccctgtcaa
    50101 ctttggcgag cgtgaccgtt ggtggggctg cctcattgaa ggctttacct accccaacta
    50161 ttgcaacaac ttcaacttcc cctattaccg cgagctcttt gaagcttacg gttttcagga
    50221 ctatttcaga caaattacct atcactcgcc tattgacgac agcaacatga gccctctcat
    50281 ccgtgaaaaa gcaaaacgtt tatcgtctaa cccggactat cactttaaac atatcgagaa
    50341 aggcaacaac gacaagtatg cccttgactt tatgaccatt tacaacaaag cctgggcgaa
    50401 atttcccgga gtgaagccat tggctgaagg ccatgtcaaa gccatgttta aatcgttaaa
    50461 gcctgtcatg gacgagaaac tactttggtt tggttattat aaaaatgagc ctatagcctg
    50521 ttttataatg attcccgaaa tcaatgaaat agtacgccgc ttaaacggaa ggtttgactt
    50581 tattggcaaa ctgaaattcc tttactacaa atggcgcaag gtatgtttca aatcttttgg
    50641 ctttgttttt ggcgttgttc cagagcaaca gcgcaaaggt gtagaagggg cattgattac
    50701 agcgtatgcc aatatggcct gtaagccagg cttcccttat agcgaacttg agctcaactg
    50761 gattggcgat ttcaacccgg ccatgatgca ccttgtagaa gaaataggct gtaaacctgt
    50821 taaaatacat gtaacttatc gctacttatt tgatcggacc aaagaattta agcgctgcac
    50881 aaaggttagc tgataatacc ccgatcaatg tgctatgaaa cgcaagattt ttaattggtg
    50941 tgcaaaaaat gatttttatt tatcgaaccc ttaccaaaaa aaatggcagt gtaaaaagca
    51001 tcattgaccg caaagtccac gaagaaaaaa taaaagttgc aaaattttac gtgcagatta
    51061 ttaacacttt gtgtgcgttg cgataaaaaa attagcaact tttactattg gctttttttg
    51121 attttttaca cacccagttt tgttatgttg tttaagtagc ataacttaaa acccggtaat
    51181 accaattatt tcacgaactc cttttaaagt ttttatagca ctttcgcggg ctttttcctg
    51241 gcccattttt gtaacctttc gtaagtacgc atcatcagag aggtactgtt tgatacgctc
    51301 acggataggg tctatagata aattaatatc tttagccaat tgttttttga ggtcgccata
    51361 acgtattgtg cattcgtggt actgctcttt aaagtatttt accacatcag gttctgaaac
    51421 tacctctaaa atggtgaaaa tattgcggat tggttcgggc atcttctgac ctttgtgggt
    51481 tggcccgcta tcagtaaccg cacgcatcac tttcttttca acagttttgg catcatcggc
    51541 aaggtaaatg ccgttgccgt cagattttcc cattttgccg gtaccatcaa gaccggggat
    51601 tttaatcaaa tcgctgccaa aattaaaagc agtaggaagc gggaaaaaat cgttgccata
    51661 catgttatta aagcgagagg caaaattacg ggtcatttca agatgttgtt cctgatcttt
    51721 gcccacaggc actttatttg cacgatggat aaggatgtca gcagccatca atacagggta
    51781 tgtcagtaac ccggcattga cattatcagg gttttggcgt actttatctt tgaaagacgt
    51841 ggtacgctcc aattcgcctt tataagcaag catattgagg ataaggtaca gttcggctat
    51901 ttcagggatg tcactttgga catatatggt tgccacttca gggtcgatgc ctgctgcaag
    51961 gtattcgcaa aggatttgtt ttacattggc cttccagtcg cctggcttgg gatgtgtggt
    52021 taatgaatgc aagtcagcaa taaagaaata gcagttgtaa ttgtgctgca tcttaacaaa
    52081 attgcgcact gcgccataat aattccccaa atgaaggttg cctgttgagc ggataccact
    52141 taatacaatt tccatagttt tcaaaataaa atgcagcaaa gctaaaaatt tctgcgtcaa
    52201 cttacaggca gaaaatcata cattggaaaa atttgggtaa cttagcgcaa ttttattttg
    52261 aatatgacat cttctgaaca aacaccacga cgactaaagg ttgcacggtt ggtccaaaaa
    52321 gaaattggca atcatcttca acgcgaagcc tttactttgt gcaaaggccg aatggtatct
    52381 gttaccaatg tgcgtatgac gccagacctt gctattgcaa gggtttatct tagtatattc
    52441 ccgtctgaca atgcagatga ggtactcaat aatattaaat tgcatataaa aagtatccgt
    52501 cattttttgg ggaaccaggt acatcagcaa ttgcgtattg tacctgatct ggagtttttc
    52561 atcgatgata gcctcgacta tgtagccaaa attgacaact tgctgaaaaa aagttaaact
    52621 tattctgaaa aaagtaatac ggatgaaata cgaaccaata aaagccagtc tgggtaaagt
    52681 ctttaacaat atacctgttt tacgcattat attttacaaa atgcttgatt tattgttgct
    52741 tcgcgcctgg catatcaaaa aagagctccg caaaataaaa aagcaaacag gcccaaatgc
    52801 ttcggtatta gatgctggtg caggctttgg tcaatatacc tattacatgt ccactttttc
    52861 aaaggggtgg agcatcacag gggttgacat tattgatgcc cagattgaag attgcaaccg
    52921 tttttttgca aagattaata aatctcagca ggtaaagttt gaaaaagctg atttgactgc
    52981 ttttttaagg cctgaaagtt ttgacctcat cacttctgtt gatgtgatgg aacatataga
    53041 agatgacagg gccgtatttt caaattttta caagtcttta aaaaaaggcg ggtgtgtgct
    53101 tatctctact ccttcagaca agggagggtc agatgctcat gataacgacg accattcttt
    53161 tattgccgaa cacgtccgca atggctacaa tattgatgaa atagccgaaa aacttaccac
    53221 ggccgggttt tcaaaagttg aatgccttta tcagtatggc accccgggta aaatttcgtg
    53281 gacgctgtca atgaagtttc ctattatcat gctcaacaaa accaaattat tgtttatcct
    53341 tttgcctttc tactatatcg taacgtttcc attttgtttc atcctaaatt attttgattt
    53401 gcagatgaaa catgccgaag gtacgggtct tattgtaaaa gcctggaaat aaaaaatgag
    53461 cttcccgttt ttcatatcaa agcgttattt tgtttcgaaa aagtcgcagt cggccatcaa
    53521 catcatttcg tggatttcgc ttggcggtgt tgctgttggt actatggctt tggtagtggt
    53581 tttgtcggta ttcaatggct ttgatgacgt tatccaatcg ctcttcaatt ctttcgaccc
    53641 tgatatcaaa attacgttga aagaaggcaa agtatttgac cttaaagctg ataacatagc
    53701 caaaattgag catttacaag aggttgacaa agtgacttat gtactcgaag agtctgcctt
    53761 gctccaatat ggagataaac aatacatagc tcaaatgaag ggggtttcgg ccaactatcc
    53821 tgagtcaaac cctataaaat ctaaagtttc tcatggagaa tttttgttat caaaaaataa
    53881 tgtaccattt gcattgattg gagcaggagt tgcctattac cttggtgttg acccaaattt
    53941 tgtaaagcct ttaaatattt atattcccag aagggatgct gacatcaacg atgctgagaa
    54001 tgcatttacc catcagttaa tttctatatc aggcgaattt tctatcgaac aggatattga
    54061 tgcgaagtat tttattgtcc cttacgattt tgcttcaaaa gcacttgagt acagcaataa
    54121 agtatctgcc cttgaaatta agctcaaaga ttattcgctg gtcgaaaaag cacaagagaa
    54181 gattagccaa attgtgggaa ccgaatttca cgttaaaaac cgctatcagc agcaggaaat
    54241 gttttacaaa atcatgaaaa gcgaaaaatg ggccattttt ttcattttaa cctttatact
    54301 cattgtggcc tcacttaaca ttattggctc gcttactatg cttatcattg ataaaaagcg
    54361 cgacatacaa acttttggtt acctgggggc caactggaaa gacattcgtc gcatattcct
    54421 tttcaacagc tggattaatg taatcattgg tgctgtcata ggtttgttta ttggccttgc
    54481 agtttgtctt gcccaaattt attttggttt tattaaactc cagggcagtg gttcttttgt
    54541 cattgagaac taccctgtaa aaattgtagc taccgatatt ttattggtct tttttactgt
    54601 cattgtaata ggttatatta cttctttaat tccagtcagg gctatttcgc ggaaatattt
    54661 tgaagattag ccttattttc ttgataattg tttgataaaa atagcagaag gccttgtcac
    54721 tcctattata gcaaatacta ttaacacaat cccggtaata ataggccagg caaaaggctc
    54781 agaaaaaatg agcaccccaa ggaaaaaggc aaaaatgata cgcgacgacg atagcattga
    54841 gcctttggtt gcatctacat atctgtaacc ggcagtcaga aaaacttgcc caacataacc
    54901 aagtaaccca gaaacgctca accataaaaa ttgccaccag gtgggcatta caaagcttgg
    54961 aataagcagt atagcattga taagggcccc tattcccatc aggtaaaaaa tgatgatgtg
    55021 cgttgagtcg tatttacggg caatgcgcaa cgaagcaatg gcaacagccc cggtagcacc
    55081 cgatagtaat ccatacaaat cgccaatatt aattgcatga aaatcgggat gtacaaccaa
    55141 cgcaatacct atcatggcaa taagcaaaaa taaatactgc tttccggcaa ccttctcttt
    55201 caacaaccaa ggggcaagaa acaacataaa caccggatat gtcatattta gcatgttggc
    55261 gttggtgatg gtagtgtatt ttacagaaag gaaaaacaat atcacggcta tggtattgaa
    55321 aaacaccctt gctccaacaa gtttcacgtt attaggcttg aaagacaatt gctcatgcct
    55381 cattcccatc cacgtgatga tgaaacccag aaaaaaacgc atgaaagttg cttccaaggc
    55441 agggatatcc gtgttggtag tggcaaattt tgcaaaaaca gtagccgccg caaagaaaaa
    55501 ctcggccgag atgaggagta tgtagccttt tttcatccgt aaataattct ataaacaagg
    55561 tcggatgaac cccgaagggc tctccgaagg aaaactcgca tgatgaattt tcatccttgt
    55621 ttgtgtttaa cgaatcatgc tcgtttcatc atatttattt ttaattttta aaaatgaaag
    55681 atagtttgct cccaccaata aatattgctg tgtttgcaat tgcgtaatca tgggtattta
    55741 atttcaaaaa atatgttttg tcccgcctta aattttatat ctttcaatta tcggaccaaa
    55801 gataataact cctcaaaatg ttaacaatgc ctgttcattt tgtaaaagcg taccattttt
    55861 taaccattgg ctaagtattt ttaagtaaaa aagttggagt tttgttttgc aaactatctc
    55921 aaaaaactac atacaatgaa aaaattgaca attatactca gtgttgttgt aagcatatct
    55981 ttttgctatg ctttaagagc acaagacatc caagtgaggg aaaggataaa ctttgacaag
    56041 ggatggcttt ttgcccttgg ccactctttt gatgcttcaa aggactttga taatgggact
    56101 ggctatttct catattttgc aaaggctggc tatggcgatg gtgcagcttc tgccaatttt
    56161 gacgataggg catggcgtgt tcttgacctc cctcacgatt ggtgcgttga gctccctttt
    56221 agcgaaaaag gtgggcatag ccacggatat aaagctatag gccgcaattt ccctgaaaat
    56281 agcgttggct ggtaccgaaa aaagttttct attccggagt ctgatctggg gaaacgcatt
    56341 agcatcgagt ttgacggcgt acatcgtgat gccattgttt gggtgaacgg attttactta
    56401 ggtaccgaac acaacggcta tgcaagttac gcctatgata tcactgacta cctcaattat
    56461 gggggagaga atgttgtagc tgtccgtgtt gacgctacca tggaagaagg ctggtattac
    56521 gaaggtgcag gcatttatcg ccacacatgg ctcaccaaaa caaacccttt gcatgtagcc
    56581 agatatggga catttattac tacagaactc agcaataaca atactgtggc aaaagctgtt
    56641 gttcgtacaa ccatagttaa tgaagcccgc gaagaacaga ctttttcttt agaagatatg
    56701 atagtggacg ctgaaaataa agcaataact tcaggttcaa aaacaaacct taagttaaaa
    56761 cctggagaaa gtaaatgttt ttatagtact tatacattga aaaaccctaa tttatggact
    56821 ttagaaaagc catacctgca caaactgctt actattgtca ggacaaacaa taaagtagta
    56881 gatacctatg aaacaaattt tggcgtacgt actgtccgtt ttgacgctaa cgaaggtttt
    56941 tttcttaacg gcaaacatgt taaaattgtt ggcaccaatt gtcatcagga ccatgccgga
    57001 gtaggtacgg ctatccctga tgggttacag gagtatcgaa taaaaaagct caaagacatg
    57061 ggcaacaacg gcattcgcac atcgcacaac cccccaaccc cggaaatgct tgatgcctgc
    57121 gacaggcttg gcatgctcgt actggacgaa aaccgcctga tgggctgcaa tgacgagcat
    57181 tttaaatatt taaaaaatat gatgctccgc gaccgcaacc accctagtgt tgtcctttgg
    57241 tcattgggca acgaagaatg ggcaatagag ggcaatataa aaggggcacg cattgcagct
    57301 accatgcagg cctatgccca acagcttgac tcatccaggg cttttacagc agcattaagt
    57361 ggtggctggg ataccggcat tggcactgtt acccaggtaa tgggctacaa ctacattgta
    57421 cagggcaata ttgacgagca tcacaagaag tttccatggc aggcaggcat tggcactgaa
    57481 gaaagcaata ctgttggcac acgcggcatt tacgaaacaa atgataatct atgccatctt
    57541 gcccccacaa accgtatgcc cgaaaatgtt gggaccgaaa gtggatggca gttttatgca
    57601 gcacgccctt tccttgcagg cttgttttat tggacaggat ttgattatcg tggcgaacct
    57661 actccttacg gctggcctgc catagcctct caatatggaa ttgttgattt gtgcggttat
    57721 cccaaagata tattttacta tctcaaagca tggtggggga aagaccctgt attgcacatc
    57781 tggccccact ggaactggaa agggaaagaa gggcaaccca taaaagtcac tgtttatagt
    57841 aactgtgacg aggtagaact ttttttaaac aataaaagcc tgggcaagca aaccatgcct
    57901 gttaacgggc atttggaatg ggcagttaat tatgaacctg gcgtgttgag cgccaaagga
    57961 tataagggag gaaaagaaac cttgcaaagt aaggtagaaa ctacagatgt tgaagatggt
    58021 attcagttaa caacagataa atcagctatt tcttcagacc caaatgatat atctgttatt
    58081 acagttaacg taactgacaa aaatggcttg gtagtcccta cagcaatgaa tgaagtagca
    58141 tttaccattg aaggagaagg cagaattatt ggcgtaggca atggcaatcc ttcaagtcac
    58201 gagaaagaca aatacaccga aaccatcaaa ttgtcaaaaa atgttgattt gaaagagttt
    58261 tctgtagtat cattagaaaa ttgcccacag gttgacccta aggtggatgc ttcaggctgg
    58321 aaggctgcct ttacaacaca aagcaaagac tggaaagttt ataccgactc gctgattgtg
    58381 gtcaggggga catttgaaat tcccgggttc actaacgaaa cgcagataaa cctttttacg
    58441 aaaagtattg ttgaaaacca gtcggtgtat gtaaatggca ggttgataaa agcaaacatc
    58501 aagcgtgatg ccgaagggca gtcatttgtg ttagatcatt ctattttgaa gcctgggaaa
    58561 aatgtatttg ccgttaccgg acagcgtttt cgcaaaaaac atcaatggga cgagcctaat
    58621 accgaccctg gccttgttca ggtaatcatt ccggcagaac aatggaaacg taaagttttc
    58681 aatggttatg cacaggtaat agtccaacct accggtaaaa caggcaacat taaactttcg
    58741 gcaagctcgg caggcttaaa accagcctct ttgataataa atgtaaaata aactacttca
    58801 aaaaaagacc tgacaagttt ttaaaacctg tcaggtcttt ttttaaaaaa catttacggt
    58861 tgaaagaaat aattaccttt gtatgagaaa aataggacgc agataattca atgcgttaat
    58921 ttttagtatg ttagataatt tttatcaacc acaatctctt gtcattgtcc ttctggcatt
    58981 attattaact gccagggttt taggtgaaat tgcagaacgt ttcaaaatac ctgcaattat
    59041 tggcgaggta atggcaggta tcattctggg ccctgccatt ttgaactgga taacacctaa
    59101 tgaatcaata tcagtacttt cagaccttgg cgtttttttt cttgtggttt tagccgggtt
    59161 agagatagtc ccacatgata tacgcacctc actaaaaggc cgaaatatct tttatatcgt
    59221 ctttgcttct ttaatccctt ttatttcagg ggctttaatt ggctatctgt ttaaaatgcc
    59281 tttatccaca tgtcttttca taggattatg ttttacggtt acggctttac ctataagtgc
    59341 acgcatattg cttgaaatga acaagctcaa caccccaaca ggtcagcgaa ttatttcaat
    59401 agcggtaata gcagatgtcc tttccctttt attgcttggg gttgtgatag aagtgaacaa
    59461 tttgcaaaat ataaaccagt tttcgcaggc tgtagggttt tcgcttctca aacttgtatt
    59521 attgttgttt atttttgtgt tattataccg tatttaccaa tatgccaagt tgcattttga
    59581 tgtcatacgc caaaaagtgg acttgttaat gcttttacta aaaggtaatg aatctatatt
    59641 tgctttggtc attgttttta tcctgctttt tgccggtttg acgcaattca tagggctaca
    59701 cttcattgtt ggggcttttt ttgcctctat gctcctcaac aggcaaatac tcgggactcg
    59761 caattatatc cgcgtacggc gtaaccttta tacagtaaca atggggcttc ttgccccggt
    59821 attttttgct tccattggct tgtcttttaa agtgtttgac atgagttact ttttgttttt
    59881 cattgtcctg gtagccgggg taaccatatc aaaaaccttg tcgggattat taggggccag
    59941 gttagccgga catttacaga tagaaggcct taccaccggc cttgggattt ctatgagcgg
    60001 gctaatgggg cttgtaattg ccaatgttgc ttacacacaa cacttcatta ataatgctat
    60061 gtattctgta ttagtctcaa tgggactttt tacaacctta gttatacctg ttttattgaa
    60121 aaaaacattt gttatactgg ataaaaaatc tgccttatgt ttggactcag catcgaattg
    60181 acccttattt tgtttttttg tttaattgcc gcctgtgcct ttgagtttat caatgggttt
    60241 catgatacag ccaatgcagt agccaatgtt atttacacca aaacgctaaa acctgcttat
    60301 gccgtaatgt ggtcaggctt gttcaatttt cttggcgtta tgctcggggg tatttctgta
    60361 gcgatgggta ttatgaagct gttgcccacc agcgtgctgg ttgatcaaaa tgtgtatcat
    60421 tcattggccc tgattttagc cctgattttt accgctatta tctggaacct tggcacctgg
    60481 tacattggat tgccatgctc cagctctcac actttgattg gctctatttt tggcgttggc
    60541 attgcttata tgctgctccc tgatgtaggt gcggtagccc taaactggac aaaagtaaaa
    60601 gatgcaggcc tgtcgctgct tatctcacct gttattggtt ttggcctggc cttgggattg
    60661 tattttttgt tgaaaaaagt gacgaaaaaa aaccaagtac tttttgagca tgacgaaaaa
    60721 cgcaaacacc ctcctgtttg ggtgcgggca ttgcttattt ttacctgcac cagtgttagc
    60781 tttgcccacg gctcaaatga tggacaaaaa ggggttggcc tggtaatgat tatcctgata
    60841 gcgtttttgc ctgccaggtt tatcctcaac cctcacctgg aaacggcctc tatttcttca
    60901 aaaatagagg taatacaagc tgtattggta agcgctgaca ccgctaaact ttcagacgac
    60961 aataaaataa agctgacagg cattcttgcc aaaacaaata aagtagaagc gattagtttg
    61021 aaaactaaaa atggccattc gcctacaata aatgacaaat atgaaatacg gcgcgattta
    61081 ggctttatac aaaccgagac aaaagacttg atgaaaataa ccgttgaaca accacaagta
    61141 tatagcaata agcaggtagc tgttgtttca acagagctgg aagcactgaa aaaatatacc
    61201 gaatatgccc cgtattgggt aattttgatg atatctgtat cacttggcct tggcactatg
    61261 ataggctgga aacgtattgt ggttacaatt ggcgaaaaga ttggcaaaac tccattgact
    61321 tatgcacaag gggtaagtgc agatttagtt actgcctcta caattgctat ggcctcaaac
    61381 tttggattgc ctgtaagtac cacgcatgtg ctctcttctg gggtagcagg taccatggta
    61441 gcccgaaacg ggctgaaaaa cctgcgtaaa aaaactattc gcaacatttt gaccgcctgg
    61501 ctgataacac ttccggttac tattgtatta tctgctggtt tattcttatt attgcgctgg
    61561 attttttctt aatgtaccat ggaataaagt tgcatataac tttttagtcc ttaattttgc
    61621 accagaaaag aaccgcaaag cggaatgaaa ccacacgaat cttctcatat ccataaagta
    61681 tcttttgccg ggttgctggt aactttaggt attgtctttg gagacatcgg gacatctcct
    61741 ttgtatgtga tgcaagaaat agccattgct accgggaaaa ttgactcctc ttatattctt
    61801 ggggtactat cctgtattat ctggactttg actttacaaa ctactataaa atacgtatta
    61861 tttaccttaa gagcagataa caaaggtgaa ggcggtattt tttcgttatt cgccctgata
    61921 aggcggcggg tacccctggc ttatattttt gccattattg ggggcagtac actgctttct
    61981 gaaggtatta tcactcctgc tatcacggtt acttctgcag tagaagggtt aaggcttatc
    62041 aatactgata tccctgtttt accagtagtt attgccatca ttacagcatt attccttgtc
    62101 cagcagtttg gcaccaagct gataggcagc tcttttgggc ctattatgtt tgtttggttt
    62161 ggcatgttag gaatactagg gtttagccat attttagata tgccaatggt gctgaaagct
    62221 tttaacccgg cttatgcatt aaaattaatt atccaacaac cttcggcttt actgatatta
    62281 ggggctgtgt tcctttgcac taccggggtt gaggcgttat attctgacct tggccattgt
    62341 gggctgaaaa atatccgtat ctcctggttt tttgttaaaa ccacacttat cctcaattac
    62401 cttgggcaag gagcatacct gctcaaccat tcatacaatg gcacaaaccc gttttttgcc
    62461 attatgcccg aatggctttt gttcccgggc attattatag ctacttgtgc tgcaattatt
    62521 gccagtcagg ctcttatttc gggttcgttt acattgatta gtgttgccat ttcactgaat
    62581 gtatggccta aaatgaaaaa gaaaaacccg actactgtcc gtgggcaagt atatatcccc
    62641 gtggtcaact ggtttttgtt tgtaggctgt atttttgtta tcttgtattt tcaggaatcg
    62701 tccaagatgg gtgctgctta cggcctctcg gtcaacctga ctatgctgat gactaccacg
    62761 ctactcatgt tttatatgat gattaagcgc atcaacagct attttattgc tttattcttg
    62821 tttacttact atattattga agggacattt cttattgcta accttcagaa atttatgcat
    62881 ggcgggtttc tgaccattat tttggccagt gtattcttcc ttgtcatgta tggttggtac
    62941 aatgcccgaa aaatacgcaa cagctttacc agctttgtca aactttcaga ttattctgac
    63001 ctcatcacgg ggctcagtcg cgaccgaagc gtcccaaaat atgccacaaa cctggtatat
    63061 ctgacacatg ccaattcgcg tgaggaaatt gaatcaaaaa tcatgtactc catgattaac
    63121 aaacagccaa aacgtgctga tgtttattgg ttcttacatg ttgacattgc tgatgaacct
    63181 gacacctttg aatatgaagt aactcatata gttccccgaa cagtgattaa aattgatttc
    63241 aggctcggat ttaaggttga cccgaagatt aatatgtatt ttaagcaggt gctcgacgaa
    63301 atggaagaaa atcgtgagat agatttggtc tcaaggtatg aatccctgcg caggtacaac
    63361 attgacagcg acttcgtatt catagtcatt gaccggatcc caaacttcga ttacgatttt
    63421 actccgcatg ataaaatgct actcaacctt taccgcttct tgcgccacat tggggtaaac
    63481 gataccaaag cctttggcct tgataccagc aatgttatca tagaaaaagt gccacttttg
    63541 gttgagaaaa gctataaaag ggtcattaaa agaaggatag tgcaaaatac cttgtcatta
    63601 gatgggtcac aagccaatta gtacgaaaca tgccactttt gccgggattg ttatgaccct
    63661 tggcattgta tttggcgaca ttgggacttc tccgctgtat gttatgcaaa gtatcattgg
    63721 tggggattcg catgttgacg aaagctttat tttaggggcc ctttcctgca ttatatttac
    63781 cctgacactt caaaccacca ttaagtatgt aattattaca cttagggccg ataatcatgg
    63841 cgaaggaggc attttctcgc tttttgccct gataaggcgc attttcccct gggctttcat
    63901 ttttgccatc atcggaggca caacattact ggccgatggc attatcaccc ctgcaattac
    63961 tgtcgttact gctattgaag ggcttgagtt atattcgcct ggcatacagg ttattcctat
    64021 taccattgcc attattgttt ttctgttcct gatacaacag tttgggactc ataaggtggg
    64081 aaaatctttt ggcccaatta tgtttttttg gttcctgatg ttagctgtat taggtgcgtt
    64141 gcaaattagt tcgtatccgg caatt
//
LOCUS       DAOSPF010000012        63191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_501_length_63191_cov_7.294285, whole genome shotgun sequence.
ACCESSION   DAOSPF010000012 DAOSPF010000000
VERSION     DAOSPF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 63191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_63191_cov_7.294285"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..2068)
                     /locus_tag="PK252_03965"
     CDS             complement(440..2068)
                     /locus_tag="PK252_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13899.1"
                     /translation="MKRLIISVLALLPIVLHAQSFWSIVDDESCHNKSHITDVALFQD
                     SMILVSGFVSDVSCSGQNLFAYNFSGQKLWSRYSVSDLIVTDKDNIYTTGYTIATDDI
                     VGDEKLVLSKYNKEGTEIFRLNYSEKTSNYSYFGFYPTGINITDDGKILVCSKTSVVK
                     SDINGSGIKKYDFGSNMDITGINPINPRFYLINTPERIYKSDSSFVLSDSIAFSGNIV
                     KAFLKKDTIYTLLSSCLVRLDTSLNIIDTVISSTLGFQNIETFDNTFWLQLKQAESIK
                     IIKTNDFKSIDTLTFPLLANDIRFIAAGKNYVFAGNSFTNQIGIYHFHADTFASEDYT
                     LPDIELVDFKVDSICIDYVKYLGDSFPRGYYFNAEFDIKNNGNDTIKGLSIFSQLHGG
                     MNCAQNYYYGKYQNLNILPGQTQTVKIHHKYEDGLNNNKLCFQCLAPNSKLETNVNNN
                     SLCKTIDITGVENVKQSKFKVFPNPMANYLMIENWDLKINHVEIIDIDGKTCISKAVS
                     GGLVKIETFNVKPGLYLLKLKSTNKVYSQLVVKE"
     gene            complement(2129..2311)
                     /locus_tag="PK252_03970"
     CDS             complement(2129..2311)
                     /locus_tag="PK252_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13900.1"
                     /translation="MGKRITNNGPSGAIYGLGMIGAAIFYISQATSFWSGVLGFLKAL
                     VWPAFLVYELLKFLAK"
     gene            2534..3631
                     /locus_tag="PK252_03975"
     CDS             2534..3631
                     /locus_tag="PK252_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT13901.1"
                     /translation="MRVIPKSIWSFLFEETKYRLPILISVFALFIGLFVFFHGKIDYI
                     VNLYFGIPVIMATYYYGMTIGVGLFATEALVHAIILTFKNEDISFFYSNHRVVIAFIS
                     LFVVVYIVSLFRKVSNRLLLSEKKNKEVSDELQKLNNSKDMFFSLIAHDLRSPIGSIR
                     SQIEMLLDNKIQLTEDERTKVLQALQSLANRVYSLLENLLKWSQIQKGLLSITLERIN
                     IQNICNDCILLFQQAAKDKEISIYNKITSELYIMADKNAIETVIRNLLANAIKYCTPG
                     GWVSVSAIEKADTYCIMVTDNGIGMEKELVENLFKIDVDVSRKGTKGEKGTGLGLILA
                     HELVDRCHGTLTVESQPTIGSVFKIELPKAE"
     gene            complement(3641..6535)
                     /locus_tag="PK252_03980"
     CDS             complement(3641..6535)
                     /locus_tag="PK252_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT13902.1"
                     /translation="MRKNFIIPIFALLVFCNTSFATNTGSSVYNVDQYTLSNGLTVYL
                     NPDHNLPKIFGAVVVRAGSNNESNEATGIAHYFEHIMFKGTDSIGTIDWAKESVLLDS
                     IRLKYDELGNTKDEKKRKEIQQQINQLSVKAAEYAIPNETDKILKHCGGTEVNAYTSF
                     DRTVYHNSFPSSQLENWLHIYYERFRKPVFRLFQSELETVYEEKNMSLDDPYSIIIDT
                     LFSKAFAGHPAGHHTVLGLSEHLKNPSLSKMYEFFDKYYVANNMALVLCGDFDPDKAK
                     VWIEQYFGKLRSGEKNFNKLPKPKGFTGREFYQYKLFPIKVGLLSYHTPESANPDNIS
                     IDLINLLLSNNSSTGLLDKLRMDNKILMAGLSPVSFPDAGADIIFFVPKILGQKLPNA
                     EALVKKEIQRVKNGDFPDELLSSLKTEKRRQLLNNKENIHGRANMLIDAFIDGVSYKI
                     KFEEDKIIDTLKKKTIVLIANKYFGDDHLVIYSKTGFPKKTKLEKPAYKPIESKEGEK
                     ASVFAKEIYNKEVIIPQPHFIELNKDVVVDSLQSNLAFFHVNNPYNNIFNINIKFQRG
                     VYYDKTLAVAANYMNLIGTENMNFDIFRKELQKLGSDIKIWADEDALCINITGFDENL
                     GQTLLLANELITKPKADEKQLMKCLDEAKLDRKFETSSPDFMGWALQQYVLYGKKSDY
                     LNRLSLKEIKKLTSAQLLASFHEAMKYQATIHYSGRLDAETVKKAIAKDIQFNSQLIA
                     FKSPVYKDRNVVTQNTIFIVNDKKAVQSKIYIFREGNVLNKDQYYLSSAFNSYFGTEM
                     SSIVFQELREKRSYAYSAYSFFDYNLSYPEKPGYLFSHIATQSDKTEEALATFYLLID
                     SMPIKPERYNIIKTNLLQSLSLKEPDFRRRSELVEKFINAGYKQDMRKDQYNYFSSFT
                     FEDIVDFYRQNVSGKPTIITIYGDTRSFNMNNLSRLGKIVKVNKKDLVKN"
     gene            6734..7306
                     /locus_tag="PK252_03985"
     CDS             6734..7306
                     /locus_tag="PK252_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOT13903.1"
                     /translation="MAEYFAHPTAVIDQGCTIGSGTKVWHFCHLMPGCVIGNNCILGQ
                     NVMVAPGVRLGNNVKVQNNVSLYSGVVCEDDVFIGPSAVFTNVINPRSHVNRKEEFKQ
                     TIVGKGASIGANATIVCGVNIGQYAFVGAGAVVTKDVLPYSLVKGNPARHAGWYSENG
                     HKLTFDNKNIAVCPETGQKYKLEKNNLKKL"
     gene            7319..8359
                     /gene="gmd"
                     /locus_tag="PK252_03990"
     CDS             7319..8359
                     /gene="gmd"
                     /locus_tag="PK252_03990"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT13904.1"
                     /translation="MAKKALITGITGQDGSYLAELLLSKGYEVHGIIRRASSFNTGRI
                     DHLYKDRHNKDVQLFLHHGDVTDSSNLNRLLEKIHPDEIYNLAAQSHVKVSFDVPEYT
                     AEVDAIGTLRFLDAIKEIGIKTKFYQASTSELYGKVQEVPQTEKTPFYPRSPYAVAKL
                     YGYWIIVNYREAYNIFACNGILFNHESPRRGETFVTRKITIAAAKIKAGLQDKLYLGN
                     LDSQRDWGYAPEYVEGMWRILQHDKPEDFVLATNQMHTVREFTELAFSHLGIELEWQG
                     KGVNEKGICKKTGKTIVEVDPAYFRPTEVEQLLGDYSKAKKLLGWEPKVTFKELVKIM
                     VEADYNTYGKKL"
     gene            8343..9437
                     /locus_tag="PK252_03995"
     CDS             8343..9437
                     /locus_tag="PK252_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOT13905.1"
                     /translation="MEKNSKIYVAGHLGLVGSALVRRLREKGYTNIIGRSIDELNLLD
                     QRDTLKFFETEKPEYVLLAAAKVGGIVANNTYRAQFIYENLQIQNNIIHASYLTGVKK
                     LLFLGSSCIYPKHAPQPLREDFLLTSDLEYTNEPYALAKIAGIKMIESYNIQYGTNYI
                     SVMPTNLYGPNDNYDLEKSHVLPALIRKMHLAKMLQNGNWEAICNDFNKRPVEKVDGK
                     AAKETILAMLNKYGISIDTSGKATVKLWGTGTPRREFLFSDDMADACVYLMENVDFKD
                     ILEKQNLTGGEIKNTHINIGTGRDLTIKELAYKVKSIIGFEGQIDWDATKPDGTAQKL
                     LDVSKIHSLGWKEKVSMDEGIAKVYKQYQE"
     gene            9560..9706
                     /locus_tag="PK252_04000"
     CDS             9560..9706
                     /locus_tag="PK252_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13906.1"
                     /translation="MDNNKLIQVAAGCGLHCGACSLYIGSTEAPERLRILSERMGQTH
                     LLWL"
     gene            9732..10043
                     /locus_tag="PK252_04005"
                     /pseudo
     CDS             9732..10043
                     /locus_tag="PK252_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
     gene            complement(10536..11417)
                     /locus_tag="PK252_04010"
     CDS             complement(10536..11417)
                     /locus_tag="PK252_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13907.1"
                     /translation="MDQKTFTQRGTFMIVLFGSFILLFTGILFYIGFDDPSKYVLLPS
                     VLLFVVCLGLFHKLTITVTDERLSFSLGFGLISKSYLLSEIESVKPVRNSFIHGIGIK
                     MLPNGWLYNVSGLDAIEVKFKNRKSVVRIGTDQPVNVSNYINSKLNNDLHQTNAEIPT
                     NSRYLLYWAILASALLIVTMFILWGNKEPKAKFENNVFELSGMYGYKVNIADISSIDT
                     VQVIPPISMRTNGYAFGNRLKGYFRLTDGNTVKLSVHLGSRPYMLIRAKNEYPIYISF
                     KDNKKTVELFKVLKGEK"
     gene            11579..14071
                     /locus_tag="PK252_04015"
     CDS             11579..14071
                     /locus_tag="PK252_04015"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOT13908.1"
                     /translation="MHYIYALFAFYHYMNQCLHKKLYTFIVFVCLSSLLSGQMTRIKG
                     IVRDAETGEPIPYASVYVKNTPLVVFTNDSGAYVFETRQKINAISVSFLGYKEATEEV
                     DKGITQIINFDLEPNVTVFEDVVVRPGENPAHRILRAIDKNRNRNSLRLNNIQRNQYS
                     KIQISLNNIDEKFKNRKILQPFKFVFDNVDTNAITGNVYLPIMIAESSIDHYSQNDPS
                     FKKDIVRATKISGIQDETIINFFGGLNQSFDVYSSYMDLYTESGFISPIADNGLLFYK
                     YYLLDSAVRAGHKCYHISFKPRRKQERTFFGDFWVADSSFALQSMTMRLNPQANVNFI
                     TDLYAEYSFYPENDSLWPIRSEFIQMDFNLAEMKRVKGVQGKKTVIYSNYKIDKPIPK
                     EIFDVSKDAEKLDSLTNSSQIDTLRPVQLSKSEQTIYGMVDSIKNVPAFKTTYNTIRT
                     IVEAHYSFEKFKIGPYFSLYSYNRVEGHRFRIGGVTNQNFSKRMRANGYVAYGTDDKE
                     YKYFANMHYVVSRIPYTRITALYRHDMSQIYLAPGELLNENIVSSFIRRYDFKKLQMI
                     DNASFNIESDIASSLSVILGTNYFKVDAGKYIPFVKPDSSEVKSINTGEIFTNIHFEI
                     GQKFFTTKYKRFRMQNEMPAFDLNLTAGIKGMYKGEYDYLKTSLTYSQYLKTNPFGYN
                     KYSVEVGKIFGKVPWPLLNVFKGSETYGFEPNGFNMMNYYEFVANEYLKIISEQHFQG
                     IFFNYVPLLRKLKLREVATCKAVIGHLNYKPEMMLLPSYMYGLHQPYVELGVGVENIF
                     KFFRIDALWRLSYWNNPDIEKFGIRLSLQFIL"
     gene            14192..15331
                     /locus_tag="PK252_04020"
     CDS             14192..15331
                     /locus_tag="PK252_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13909.1"
                     /translation="MKSKRLMIIFLLSVPSLIIIGILFMQQKAFGQQPKEERLQKILL
                     SPNYKDGKFQNMEETPVMSEEGMTYKFLFEMFKRHKNTKPSLPIPSVKVDLKQLVGGS
                     TDIVWFGHSSYLIRTNKTNILVDPIFSKKASPVSFAGVSSFEGSLPYAAADMPDIDIM
                     VITHDHYDHMDYKTIMALKDRVKFFVCPLGAGQHLDKWGIAKSKIIELDWWEGKEIVP
                     GIMLTATPARHFSGRGLVRNKTLWASFVLKTDILQIFIGGDSGYGKHFKAIGEKFGAF
                     DIAMLECGQYNKFWPYIHSMPEETALAAKDLNTKVLMPVHWGKFKLAMHPWYEPVMRV
                     GAKAAELHIPIVTPVIGKPFILENIVNEPLWVNELLKNDNNVTLN"
     gene            complement(15328..15696)
                     /locus_tag="PK252_04025"
     CDS             complement(15328..15696)
                     /locus_tag="PK252_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13910.1"
                     /translation="MIFQLSQLLQKLKIFILTKAIVTWSWIKSVGRAFKRESEETKIA
                     SKILLKMLKGKDVTPEEVQFLKGQSVDLGKALALIGLQAIPGSSFAIIAIEKVAQKYG
                     FTLFPQEQKELKKSANKKNP"
     gene            complement(15693..16322)
                     /locus_tag="PK252_04030"
     CDS             complement(15693..16322)
                     /locus_tag="PK252_04030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOT13911.1"
                     /translation="MNSNEVYPRKGDKETVYLRPTITKPNIIVGEYTMYNDFVNDPTQ
                     FENNNVLYHYPINNDKLKIGKYCSIACGAKFMFTSGNHRMSSLSTYPFPLFWEEWGLE
                     RNKVTDAWDNEGDIIIGNDVWIGYEAVIMQGVQIGDGAIVATRAVVTKDVPPYSIVGG
                     IPAKVIKKRFSEQVIKKLLDIQWWNWDEDKIKSNLPHILNGNVDKLTHL"
     gene            complement(16367..16732)
                     /locus_tag="PK252_04035"
     CDS             complement(16367..16732)
                     /locus_tag="PK252_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13912.1"
                     /translation="MNPKIDKIKEMLIAQYSKLCGLSIEATETKLANIDWHSEFQKLK
                     RESKRTKYAYFIFISACTLILIFALISFQNNISVSKFNNFTLIFSGLFFLSASCYSVY
                     NKINEKIRILGLLFEIYKS"
     gene            complement(16833..17957)
                     /locus_tag="PK252_04040"
     CDS             complement(16833..17957)
                     /locus_tag="PK252_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT13913.1"
                     /translation="MKNFKSGQYISQGYYKSFQPEFINRQLHIDNMEVMQLLSQADRE
                     LGRLDMYSKYIPNIDLFISMHVLKEATQSSKIEGTQTNMDEALLDKEDIPMEKRDDWE
                     EVQNYIKAMEWAISELEKLPFSSRLIRETHKVLLQGVRGEKKQPGEFRISQNWIGGAT
                     INDAIFIPPVHTSVPELMSDIEKFLHNEELFLPELLKIGLVHYQFETIHPFLDGNGRV
                     GRLLIPLYLVSKGILQKPILYLSDFFEKNRALYYDNLTIVRDKSDLNQWYKFFLVGII
                     ETAKNGIETFDNILQLQKKTEVNLQSLGSRAAKAKKIVDYLYKRPVLNADKIHEIADI
                     SMPSSYKLISDLEKMDILKEVTGGQRSRMYVFDNYIKLFR"
     gene            complement(18230..18529)
                     /locus_tag="PK252_04045"
     CDS             complement(18230..18529)
                     /locus_tag="PK252_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT13914.1"
                     /translation="MEKYSKIKNFDELIELEHGKLGTESRNKYEEQAQMFIVSEMLKE
                     ARREAKMTQEQLAIKAGTKKSYISKIENAKGNIQLSTLIRIFEKGLNKRIGLTFL"
     gene            complement(18532..18871)
                     /locus_tag="PK252_04050"
                     /pseudo
     CDS             complement(18532..18871)
                     /locus_tag="PK252_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(19134..20348)
                     /locus_tag="PK252_04055"
     CDS             complement(19134..20348)
                     /locus_tag="PK252_04055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT13915.1"
                     /translation="MNFIDFNFTSDLQQGLDAMGFNNPTPIQEQAIPVILSGKDLIAC
                     AQTGTGKTAAYLLPVIHKLVEQKASLDTFKCLIIVPTRELAMQIDQQVEAFTYFCQLS
                     SISVYGGGEGVLWDIQKKALTEGVEIVIATPGRLIAHLAMGYIDTSNVQYLILDEADR
                     MLDMGFHDDIVQIISYLPKERQTLLFSATMPEKIRALAKKIMGDAPEQISLAISKPAE
                     GIVQAAYFVEDHFKIGLIKELLKEKKNINAIIFSSKKNTVRIIERELLKLGFDVASIH
                     SDLEQKQREQALLDFRNRKLQILVGTDVVARGIDIAGIGLVLNYDVPPDAEDYIHRIG
                     RTARAETTGVALTFVNRADRHKFRQIETFLEKQVFKIPVPKHFSTGEEVVDEEHAEPK
                     KKFFKKRWFKKK"
     gene            20489..20881
                     /locus_tag="PK252_04060"
     CDS             20489..20881
                     /locus_tag="PK252_04060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT13916.1"
                     /translation="MESKLVKSFLNDRFAEHVGIKLVEIGNGYALAELALTEKHLNGL
                     DKIQGGVIFTLADYAFGLASNSQGFITLGLNVNISYFKSPTGKVIRAEAKEISAQNKI
                     CGYQVNVTDEDGSLVASFNGLGYRKILN"
     gene            complement(20882..21124)
                     /locus_tag="PK252_04065"
     CDS             complement(20882..21124)
                     /locus_tag="PK252_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HOT13917.1"
                     /translation="MQDINPVNPQLLLELVNTKMPYGKYAGRLLCNLPEPYLVWFKQK
                     GFPEGKLGMQLQTMYEIKLNGLEYLLKPLIQQGRNR"
     gene            complement(21343..22290)
                     /locus_tag="PK252_04070"
     CDS             complement(21343..22290)
                     /locus_tag="PK252_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HOT13918.1"
                     /translation="MKTFINPPFISALITGWRFAKKGYSGKPQKYIRKQQLKIVYFTL
                     LNPGFANAWFNFLKSSKFRHVFIHRRRLYVKPFRPYISIKWDKERKVNVILDTYRFLE
                     SKGDVFRQFLTQKDGIVVAKLKFDNKYEGFIKLGYNYAIRKEGELALWLECDKLGGRI
                     TSVVFSIEEKTKGQWVCLIGCIQGHKNKAVQDAFKVMQKSLYGLRPNSLIIYSAQELI
                     RNMGFSAIYGIGNSIHISNQKHAVNFPWKRNITFDYDNFWDEIGGRSINKDWFEIPLR
                     PTRKNIQDIKSHKRSMYIKRYEMLDNLSQQITKLAETLN"
     gene            22670..26707
                     /locus_tag="PK252_04075"
     CDS             22670..26707
                     /locus_tag="PK252_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT13919.1"
                     /translation="MPGKKERQNSFFLFQKITVKAFLLCLTFVLEAYPIFANKKFNFK
                     HLTIENGLSQSWVRCILQDKYGFMWFGTDNGLNRYDGSSFVVYMNDPANSKSLSNSRI
                     NALYEDKDGNIWVGTLNGLNLFDRKTNTFSCDPSWPSSRVTSIAADKQENLWIGTHSG
                     LYFLDKHTQKASYCAYATDKNKVNNLAINKVYVDKENNVWIATMNGLNVIKSKTKQVI
                     FYTSDKNRQNSLSHNDVRCLFEDRQRRLWLGTAKGIDIFTNAADVPETAHLIHIQNNS
                     NKKSLNEGAVQVIFQDNKGRILIGIENGGLDVLHPQNLANGKFEFDHHVNIADDDNSL
                     SNNSIYSIFQDNQNSIWVGTHGDGLNIYNPLNEKFELVQNSTKTSRLIINNQVNAFVE
                     DEKYLWVGTEGGLSCYDKKTRTSRHFEHNPANKKTLRANAVLALLKDKSGHLFIGGME
                     MGLSQFDFASGHFIHYKANPSDTNSLRSDNIFALYQDSKGRIWAGQNCGGLSLFDFTT
                     KKFKTFRKNKDADDCIKAIAETPDGKLWLINATSIECFDPESGSFTTYTNNPSDPESL
                     SSNYGFSIFTDSKGALWVGTDAGLNVFDYKTKKFKKKYTIKNGLPDNAIKSINEDNFG
                     NLWIGTDKGLSKFVNATEQPSNPRFINYKVQDGLQANEFRKRATYKNAQGKLYFGGIN
                     GFNAFYPDSIAENKAIPNVVITELLIFNQKVNVGDADSALKEDISIAKEITLSYRHSV
                     FTLKFAALNFIAPAKNQYAYKMEGFDKEWNIVGNKNEATYTNLSPGKYTFVVKASNND
                     GIWNQKGRSLTITILPPWWKTWWFQTLFYLFIGFMIFAIIQFRTAFYKKQNEKLAQMV
                     KERTLELEEINVNLEEKQEEINEQNEELLTQRAQLEHTNELLVEHQLQISQQNEELMS
                     QRDALEIANKELEKQKAKILEQNIELDKHRNELESLVEIRTQQLIQAKDKAEEADRLK
                     SSFLANLSHEIRTPLNAIIGFSNLVIDGIDTPEEKENIRQIIYSSSESLLGLINDIID
                     FSKIESGHIDIILKEVPLKKIMADIQSIFQLQLKRQQIGTSHNIDFRVIANEQLNDIY
                     LETDEVRIKQILSNLIHNAIKFTLKGSIEVGCHVLGDNEIVEFYVKDTGIGIKKENLQ
                     IIFERFRKIEDDKTHLHRGAGIGLAITQQLVSLLGGQIRVESEHGIGTTFYFTIPVKK
                     NPEGNQTQSSNAIAENITDFKNVKVLVAEDDLANYAYIERLLLRFNINVVHALNGTQA
                     IYLLTKIPDIKLVLMDIKMPLLDGIQALHQIKERKIDIPVVAQTAYALSHEVVKLKEE
                     GFDDYIAKPLSLADISRVLHRWIKI"
     gene            complement(26926..27378)
                     /locus_tag="PK252_04080"
     CDS             complement(26926..27378)
                     /locus_tag="PK252_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HOT13920.1"
                     /translation="MKTFKYIALLAITLFTLACEKDEDRVVLNSNPGTPDIITPAPNS
                     AYVLTAANDKQTIQFVWKEPNYGFSASAAYNILADTPEGNFVKPQKLLSSVSYNDSAL
                     VTYKALNTAALALKLKAGEEGVLKVKVISVLAGDTLHSAAINIKVTPY"
     gene            complement(27406..28956)
                     /locus_tag="PK252_04085"
     CDS             complement(27406..28956)
                     /locus_tag="PK252_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT13921.1"
                     /translation="MKKNIVFPIVASLILISSCTKDLDVTPIDSKLATANNVFKTPDD
                     YKQALAKLYTSISLSGQQGPAGQPDIAGLDEGFSNYLRLYWNCQELSTDEAVNCWADG
                     TIKDFHWQTWTPNNEFITTMFSRLYYTISLCNEFIRTANDNSSVQGMGLMKAEARFIR
                     ALSYWHAIDLFGNVPFVTEADKPGFFNPKQVNRADLFKYIETELLEAQNDLGEPRFEY
                     GRADKAAAWTLLAKLYLNAKVYTGTERNTECITYCKKVIDAGYSLAPTYSDNFMADNN
                     TSPEIIFAFTYDFFHSTGYGGMNYIIHAQVGGSMKADSFGIASGWGGNRVTSAFVSKF
                     PDVTGTIDTRALFHTDGQTLEITDIANFTNGYAVTKFRNMNKDRKLPKDRGDFVNTDW
                     PVFRLADVYLMYAEAILRGGSGATIDDAITYVNKVRERAYKGTSGNVSTIDLQFIIDE
                     RSRELYWEGHRRTDLIRFGQFSKSTYVWPWKGNVAAGVSTDEHLDLYPIPASDLNSNQ
                     NLKQNPGY"
     gene            complement(28993..32004)
                     /locus_tag="PK252_04090"
     CDS             complement(28993..32004)
                     /locus_tag="PK252_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13922.1"
                     /translation="MKKILLSIMATIIGLTSFAQQLTIKGTVTDAKDGSPIPGVNISI
                     KGTTKGTLSSFDGVYSINANPNDVIVFSFIGYENQEVVAQTSTTLDISLKESVTKLDE
                     IVVIGYGTVKKSDATGSVAVVSTKEFNKGAVTTPQDLIIGKTAGVVITTNDGSPGGGA
                     TIRVRGGSSLTASNDPLIVIDGVPIDNTPVSGQPNSLSTINPSDIESFSVLKDASATA
                     IYGSRASNGVIIITTKKGKEGNIIVSYNNTTSVSTVIDYYDVMTGDELRQLALERSTI
                     KGSGVTAASLQRLGTANTNWQKEIYQNAFNQDHNLSISGGIKKMPFRVSLGYTDQDGI
                     LKKKNMDRKTLALAIDPKFFDDHLKVNLNVKGMYTDNNFSVDDAIGAAVAFDPTQPVM
                     NGNTRFGGYTTFVNISEVNPDGSINVNGDPNAIATKNPVAMIEQTDNKATVKRSIGNA
                     QFDYKFHFLPELRANLNVGYDLSSSKGHNNCDTIAAFTFRDGIGRKQTYTQTLKNQLL
                     DFYLNYVKDLSAINSKIDITAGYSWQYFYREKEDIKQNLVAPIKIDTTGYKSENYLVS
                     FFGRINYTLLDRYLITFTLRNDGSSKFGKDSRWGLFPSVALGWKINDEAFLKDVSWLS
                     TLKLRLGYGITGQENIGDPEKSYTYYPYLATYQSSSSTAQTQFGNTFINTSRPNPYDA
                     RIKWEETSTTNIALDYGFFKNRLNGALEVYYRKTNDLISNIPIAAGTNFSNYLTTNIG
                     NLENKGIEFSLNGVIVSQKDYFWEIGYNIAYNENEITKLRNIEDPTFRGMDYGDISGG
                     VGNKIQIRAVGQPTNSFFVYQQVYDSQGRPVEGLYVDRSGKKGNVSGNNLNKYYYKKP
                     APDVTMGISTRGNIKKVDFSISSRINLGNYVYNNVLSEKANYAQLYLQSGYFNNLPKA
                     VSKTNFASPQYWSDYYVENASFFKLDNISVGYNFDKIITNKLSGRLGFAVQNVLTITK
                     YSGLDPEVESGIDKNIYPRPRTYLVSLSLNF"
     gene            complement(32238..32486)
                     /locus_tag="PK252_04095"
     CDS             complement(32238..32486)
                     /locus_tag="PK252_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13923.1"
                     /translation="MKIVNSTIYFDELILNSMFYCQRGKHGCNCFFQWVQKKSTPDKA
                     AWFRSLSDHDKRELVISHLKCKATLNDNYKAIAHWQNN"
     gene            32655..33056
                     /locus_tag="PK252_04100"
     CDS             32655..33056
                     /locus_tag="PK252_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT13924.1"
                     /translation="MAKIVNLSEASSIALHAMILIGRAKGGSINVDQIAEVINSSRHH
                     VAKVMQRLVKEGFVNSYRGPHGGFFLVRKPEQINLLEIYEAIEGKVSPSACPMDKPVC
                     NFGMCFMDNIARELTVQFTDYLKSQTLAKYL"
     gene            33263..36949
                     /locus_tag="PK252_04105"
     CDS             33263..36949
                     /locus_tag="PK252_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT13925.1"
                     /translation="MRTNATKNFVDQTIDALSNNSDTLPEKEKDVYTLLENVSVAKDI
                     LESELNKKENEIKPNKGKNCIQSFVNTLCGYIGAYQVFIGRMSESIEGIVEIILSNDI
                     ENADKIKHVKIEGTPFSKCKEIDMLVCTEAPLKSFPGLTKITSIEPKSLIQYVHKKSG
                     AVCLALFNSYAMDVSKTEEVLESFIPEMVNFVNENENINNIEQSLARYKHIVETLSEY
                     IVINKIENGKVINISHSDICQKVTGYSMEDYKNDPDLWLKMVYEADKRYVTEQLNKII
                     FGIEVKAFEHRIYHKNGTVRWIRNTPVLFYNAMHQPVEYVAIINDISELKQVELKMIE
                     SEKHYKMLFNEMNSGFGLHEMIYDENGNPADYRFIEVNLAYERLTGLKREAIIGKRLL
                     EILPGIDKEWIMRYGQVVKTGIPETFVKYEPLFDRHYEVVAYSPDKDLFAVVFNDVSD
                     RVKAREQLEERENRYRSIVELSPDAIVIFANEEIVYANNKAANLFGFCSAQQMQGRNV
                     KEFFRNAYIDEVKLNVVKEFEEKLPGRLVEEQIKKQDGTLVEVEISAAPVFLGKKTIQ
                     LIIRDISQRKRTEAALIRSEERFLMAMDAVNDGVFEWDLKANEVYYSPRNYNMLGYEV
                     NEFEPKHAVWESMIHEEDKANNLAEFQRHLRGESEMYELEYRVRTKSGQYKWISERGK
                     ILERDKTGNPIRIIGIHSDIDARKAMEENLRNEAEFINNLMNIAPVGIFIVTVDGKIP
                     FANDLAIQMIRLKPGQDGYYNVPQYKVYDSDGKEVNFQDLPIYNAFKSMTSALHQRFW
                     YESEGKKIFVETNMRPSVSLYQKKEGFIIVVSDITNQIDAENKLRQAKEKAEESDRLK
                     SAFLANMSHEIRTPMNGIIGFSTLLAKPDLTQDKRDNYLNIVQNSANQLLSIINDILD
                     ISKIEAGQMQIDHSHLKLNDLLDSLHLQFIKEKKDKGKEHVGLFLKKGLLDEASHIIS
                     DKIRIGQILSNLLGNAIKFTEKGSIEFGYTINNNKWIEFYIRDTGIGIPAEKGKLIFE
                     RFRQIDETTARTYGGTGLGLSISKGIVDLMKGKIWFEYEIGVGTVFYFTVPYNPLKNV
                     QQTETVKLVSADENHSLEGYEVLVVEDVPENFYLLREILEEAGAHILYASDGKTGLSI
                     LKNTEKIDFILLDIRLPDISGYDLARQMRKEKPDVPIIAQTAYGLSGDREKALAAGCV
                     DYISKPIVADELMKVIKRNITPKQ"
     gene            complement(37124..39442)
                     /locus_tag="PK252_04110"
     CDS             complement(37124..39442)
                     /locus_tag="PK252_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT13926.1"
                     /translation="MKQACLELNSNTYESRFLSTSSSAFTEQAMRTLENNFVPMGTSN
                     LKKCRVGLLFFVVLFFAMTTSAQSFTIKKKVADRYNNRFEYYKAIPMYHELHDAQPYN
                     MEIVYKLANAYYRINDSQHAEYWLKLITDSFPNDTISVLRLAQVLARNGKYNQSKEWY
                     EKYAILKPADAQGEAFSSAYNNVSIFYKDSASYLLKNMPFNSSFSDFSPAYYKNGLVY
                     ASAREKKGIIRLWYSWTNSSYLDLYFALVDSSLSTEFMPGLNSLYHEGPVAFNKTFDT
                     IIFTRSNFHNLLFGKSKEKINKLKLYQANWDSTKKKWVNVVPLEFNNNEYSCGHPALS
                     ADGNTLFFVSDMPGSIGGTDIYKTHRVKNENGYFHWSKPQNLGSVINTAENEMFPFID
                     NEGNLFFASNGHAGLGGLDIFMAKTINDGFENPVNLGFPINAQSDDFGFVISPSGNEG
                     YFSTDRFNDVGNDDIYRWKKISRNLVILTYDKKNLQPLSSVNLIISNENSDPENKTTN
                     ILGLAPLVVKPSKDYQFTATRDKYIETKVNFTASELTNLDTIRIPLEKRIPKFVINGK
                     VYAADDKQPFANIKAIVTNLADSSKTELVTDASGNFIADLQPEANYNIAVQISVKGKK
                     CSANAVNISTKELEDDNVFTEMFPVLCVGDVIRVENIYYDLGKWDIRPDAAKELDKLL
                     DMMNNYPKMRIELRSHTDSRGSDAANLALSDKRAKAAVAYLISKGIAPERIVGKGYGE
                     SIPLNKCKNGIKCSEEDYQVNRRTEFKILSIE"
     gene            complement(39728..40663)
                     /locus_tag="PK252_04115"
     CDS             complement(39728..40663)
                     /locus_tag="PK252_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOT13927.1"
                     /translation="MTKYFKISVISIIVFAANVNAQQDPLYAQYQFNGLVINPAYAGT
                     HEVLSATLLYRNQWVNMPGAPKTFTFSADAPFRKNKVGLGMTVIADKIGITKRNALNG
                     IYSYKIPMKKATLALGVQMGLSFFSSNYTDVNYDNRNRSTDNAFSQNTSNTYPNIGFG
                     AYYFTDKYYIGASMPSFINSSLLEKYFSKPDAYNFSQSNHMFITGGYVFDINSDLKIK
                     PSALMVYVYGANPQVDINTTAWLFETLGLGVSYKSFDAVNAFTEIKVLPQLYFGYAFE
                     YSLTNLRNYNSGTHEIMLRYDFDFAHPRVITPRYF"
     gene            complement(40675..53310)
                     /locus_tag="PK252_04120"
     CDS             complement(40675..53310)
                     /locus_tag="PK252_04120"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT13928.1"
                     /translation="MKRVFEKISRIAIIMATLFLISKSIYAQEICNNGIDDDADGMID
                     CFDNQCYTSPYCALPSAQCEITADPGNAEFATKLECAYLKGGFMVGGVIRNFTPYGYD
                     TPKVGDVDGDGKVEIIAVGDGYGGTGDEGVYIFDPSNCEPKYYIARDISGNHGGISFA
                     NVDADPYAEIFVTTGNDGCATNANSLIRYDFDGIAWNQTWIISNIYQKRGFPVDFVDF
                     NEDGTSELVVYGWTTDATRTAKVLRASDGATLVDLTTNATFRNRLYWKDVFAFADVIP
                     TGFDPDGAGPKTALQYANGPEIIAGNRIYTVDIASGNIEDVYGVDPLTSTGSASDHFP
                     WSYNHGDATNPANQQDNDGGQKNSLADINMDGVMDIITVGQGRLSVWNPLTNQRLYPT
                     FVLAGDNLTAGKHDGGVACIGDVDGLPDNRPEMGIVSTRRFEMIRVNDAGTALQRMWQ
                     SATTDQSGETAMNFFDFEGDGKVELVYRDQDTLKVYVGVGDGAGNAKILFQDANHCGS
                     GTGGEHAVIADIDDDNRAEIIASCTEGIRVYRDNRTPWISARKVFNQRAYNYVNINND
                     LTVPKVQQKNHLVPYLNNYLTQLYRIDQSGDKYFPGPDFTVEVEDVTSDVDISSSCPD
                     KVIIRLKICNRGDALANYTIPMSFYQNDPRNAGAIFLKKSMITVTLNASECYKTEPIT
                     VSLSDLVNSNGANDGNLYFVVNDDADGLAPNQVISNPISIPNSPYGECNYSNNIYGPI
                     KIVDCHFVPIIHLLTTDVTLEDQPLTFTSLSSNQILVTDYDNDAQTVTINVTGGTITL
                     SGTTGLTFTSGDGNSDASMIFSGSLVDVNNALNNAVFTPESNYSGTATVQVKSSDGLG
                     EDVKIINVAVTPVNDNPVINDVNMLCAEDNFITFAPSDFTNSYSDPDNDALNKIKIIT
                     LPPYGVLKLSGVAVTAGQEISFAQLGNLIYVPDANWNGAITCDYNSSDGTLYASTSKK
                     IWLTVTPVNDKPVLSDIYKSSAVNAATSFATSDFTASYFDADANPLEKVQINSLPAKG
                     TLYYGGSPVGLFYEIMSANLGLLTYQPNTGYSGTDEFNWNAYDGTSYALTGAQVKFVI
                     SNTNVAPVISGFIKNGTEDNELSFAATDFLGVYSDANADALNKIRIETLPGNGTLLLS
                     GVAISAFQEITATNLGNITFAPNANWNGSTSFSWKAYDGLTYSSSSANVTINISAVND
                     LPVVADFSKAGTENITLAFTAADFTNAYTDIDNDALAKIRVVTLPANGTLKLSGANII
                     ANQEIIAANLANITFVPNADWSGNTSFTWNGNDGSAYASSNMTVNIVIANVNNAPVLA
                     GIEAGALSFTEDELPKIITSSITISDIDNTTFRNATIKITGNYNSGQDVLSFTNTATI
                     TGTWNLATGTLNLVGTDTKANYEAALRSVKYQNTSDDPATSVRTVSFTISDGSGSSNT
                     QTRNINIVAVNDAPILENIETTALVTPQLVNKVISNTITIKDFDDSYIESASVKLSIN
                     YVNGQDVLQFTNTATITGVWTPATGILTLTGTDTKANYEAALRSVTYRNNSSTPNTNA
                     RTIVFTVNDGNLNSNIQFRSVNISVANNPPVLAAIEGAALAYTENAAATNVTASMTVS
                     DDGPNLQGAEVQITGNYQSGQDVLSFTATGSIVGIWNAATGKISFSGTDTKANYQTIL
                     RSVKYANTSENPSTLVRTVSYTVNDGEYNSNVQTRNINVTKVNDLPVVAALNKSANVS
                     TDVTFTLADFTSHFTDADGDAMTQIQIGAALPANGTLYRDANSNGVVDAGEAVAANAV
                     ITNANIPFLKFKPTAAWSGTTTFNWNGYDGTAYAATAAAVNIAISNNTPPVIQAFSKS
                     GNEDTDLAFAASDFGNAPTYTDAESNAMANVQITTLPANGTLYRDANSNGLVDAGEAT
                     TVGQVYTSAQLALLKFKPTSNWNGSTSFTWMAQDNNAIPKYSTTAVNANITVTAINDA
                     PTTANFSVTTLEDVDKVFAISDFPYMDVDAGDVLVRIRIVSIPGTGVLYNDANLNGIV
                     DGGETLAATNIVSATDINAGKLKFKPVANGNGTPYTTFQFNVGDVSAYSGNATMTINV
                     TPQQEAAVLSAIEGTTLNYTENAGPMAISSTINLTDIDNTQAQSATVKITNNYQYGQD
                     FLSFSDNIFISSSWDQATGTLTLTGPADISYFISALRTVYYQNTSDDPSTLTRTISFN
                     VNDGTVNSNTISRNISIVAVNDAPVLANIESASLLYTENDAATNITSNLTLADPDNTD
                     IQGATVQITSNYQNGQDVLSFINTGSITGSWNVATGTMTLSGTDTKANYLAALKSVKY
                     VNTSENPNISNRSVSFSATDGLLNSNTVSRTIQINSINGAPTSANNTVTTNEDVDKIF
                     SDTDFPYTDIEGDALAKVMVTSLESAGSLYLDLNGNSVINAGEDITLNQEILVTQLSF
                     LKFKPAANANGTAYATFGFKVNDGSVYAVAENTMTINVLAVNDAPTSADNAVTIVEDN
                     QKTFASAEFPFSDIDAGDVLTKIRIASLPGAGTLFNDANLNNVVEAGEILAVNDEVTK
                     AQLDAGYLKFRPVPDANGSPYVLFQFDVYDGVSYSASSYNMTINVTAVNDAPTITSSS
                     TVSVAENTTAVIAVTAYDPDAGDPKTFSISGGADQALFSINALTGDLSFISGRNYENP
                     TDANTDNIYVVDVRVTDGGGLTNTQSISVTVTNVNEAPTVADFTVNGTEDNTFIFAAT
                     DFTSNYTDVEGSALSSIKVITLPANGILKLSGVNIAINDVIAFANLGNISFVPTTDWN
                     GSTSFDWNASDGSLFALANKTVTINVTAVNDAPVLAAIEAGALTYTENAVATAITSTL
                     TVSDADNANMASATVQITGGYQSAEDVLSFTNTASITGTWNSSTGTMTLTGSDTKANY
                     QAALRAVKYANSSDNPTTAARTVTFTVNDGTAGSNTQTRTINITAVNDAPTASNSTVS
                     TIKNIQKVFNVASFGYSDIEGDALTLVKITNLPASGTLYNDANLNGSIDAGEAIAMNG
                     TINKADIDAGQFKFKAPFNDFGAPYASFDFKVYDGIDYSVSSYTITININSSNTAPTA
                     VNKALSTNEDTYLSIVTADLGYADVDGDVLNHLQITMLPAIGVLFIDANSNNILDAGE
                     PIALNGTISKADIDAGKLLYSPASNGNGISYASFSFKVNDGNVYSTSDYTITIDVTAV
                     NDAPVVSDFTVNGTEDNIFSFAVTDFTGNYADVEGTALSSIKVISLPANGLLKLSGLN
                     IAINDVIGTADLGSITFVPSANWNGSTTFDWNATDGALYASANKTITISIAAINDAPV
                     VNDTTFFIAENTATATSVGTVSATDADADALTYSITSGNTGTAFAINASTGEITVNGA
                     LDYETKSSYTLTVHVSDGVTSDDATITINITNVNDNAPVSTVDSYTVAEGGSLNISAP
                     GVLANDNDVDGDALFAVLVSGTTHGSLAFNADGSFSYTHDGTETTSDTFTYKANDGLL
                     NGNTVSVSVSVTAVNDAPVLTKDTIKMSIPINKDTTFDVCSNVTDAEGATPTIIIGCA
                     ALHGSASVSGCNITYNPIAGYSGNDTICYTACDNGTPNKCVTGYIVIKINSAPSDNAP
                     IIDPGTTFVVSTTEDIPQVVIVVSNSVSDPDGDSLVFSISDAPKHGNAIINANGDVVY
                     VPAKNYFGNDTIVFTACDITTPVQCVTDTIYITVANVNDAPVLDNDTILKNTNENEAV
                     IIDITAISFDPDSDILTTSVVNGPSNGSIKFKGNDIVYTPSTGFAGNDTLYFSVCDNG
                     APKLCSSGMIVISVINSNKAPVAVNDIFEINEDNTFSGNVLANDFDPNGGSLTALVSI
                     NPVHGAIMLNFDGTIIYTPEKDYFGKDSVQYLICNNGSPSLCNNAWIVFTVKPVNDKP
                     VCSVNNGVTNENTALNIDLLSNITDIDNSSSDLIVSVLVNPTGGTVKIYNGTLIYTPN
                     IGFTGQDTIILNICDNGIPSQCTSDTIIIHVLPVPAIAIAKSVATPDVQADDSYDITY
                     TIYVKNMGSTKLNDVLVGDNLISVFPEPAIFKVKHNSISANGNLIVNPNYDGAIDTML
                     LLEGSTLEGNEMATITFTVNVYVEGDETTTITYNNIARASAKSINGKTVIASSINGII
                     PEIANGEPTPVNIKPVEFFVPEGFSPNGDGYYDNFIIRGGQRYIIHLQVYNRWGNLVY
                     ENKNYKNDWDGKTNIGITVNSTLPDGTYFYIAKFNKNRKPIQGYITINK"
     gene            complement(53351..53491)
                     /locus_tag="PK252_04125"
     CDS             complement(53351..53491)
                     /locus_tag="PK252_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13929.1"
                     /translation="MKGKHNNRLISSSAELTEALKKMIESIEHLEKEGHYTKEKNKQN
                     DK"
     gene            complement(53896..55119)
                     /locus_tag="PK252_04130"
     CDS             complement(53896..55119)
                     /locus_tag="PK252_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT13930.1"
                     /translation="MNVPRILIVDDLPEMLMITSKSLLTSGEKYEIREALNGKQAYET
                     MGQWTPDLVITDWDMPAMSGLELISKMKQDETFCDIPVIMITGMMVTPEDLKEAFRCG
                     ASDFLRKPFDKTELLARTKAMLIVSKSIKDKKNYNLVLSDYNRFINSLLLAIPSPMVY
                     YNKDGIVLGCNESFQKTFSHISSNFIGTEVYTICPNPEQMKAQSEDMINKGLQQTSFE
                     TVMKTVNKEDATFLVTKTIFYSPDKKPEGLICLLSDISEIKQEHARNLEEKKKELVST
                     AMRLMQYSTMNNKLLEDLEILDKHTDEEGKKLIAEFKSKYNIKVYNSMWEEFETRFGQ
                     VYEDFYHRLVKLHPELTPNEKKLCALLKLNLSSKDIAALTLQDAKSVDMARYRLRKKL
                     GLQQEDSLVEYLQSI"
     gene            complement(55134..56885)
                     /locus_tag="PK252_04135"
     CDS             complement(55134..56885)
                     /locus_tag="PK252_04135"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOT13931.1"
                     /translation="MHPLSLILLLTSFINLLIAFYTIRYYKSPGALAYLSILLCIALY
                     AFGYVMELNSDNLEDIYFWLKVEYVGIALFPSVLVIFTLHYTGYNQYLKKWMIVVMIA
                     ISCTTIVLQNSNFGHLFYTKLAINTAQSSSFANFTKGPWYWVHQVYSNLTLLFSFLLY
                     VWMAIKQVGINRVRAFLMIVSLTIPWGFYIYYLSSYYKSNMDIIPFSFSIVGIMGALG
                     MFRFKLLDFEPLALEVIFESMSDGVIIVDKKLRITNFNNHAKQIFNNLNEKSIGKSIE
                     KILEQCEDTKRIAFRHEEYSTDIENDCFGGRRYFLIKTTPVFENKDRLAGWAIILSDI
                     TQRKLNEVALKENAKKLKELNATKDKFMGIIAHDLRNPFHVLINLSEMVQQDIADNNY
                     EDAGKCAEMLYETAKNTHTLLQNLLEWSNFQRQGVRFNPEWIDVAEALKDDLNTIELL
                     ASQKKITIKKTISPGLKAYADSQMLKTIVRNLMTNAIKYSYTHTEVFLQIIEETGNIK
                     ISIQDCGVGLSQEDLSRLFKFETSFSKSGTASERGTGLGLVLCAEFVALHKGQIWAES
                     IPEKGSTFNVLLPLNNN"
     gene            complement(56959..58425)
                     /gene="crtI"
                     /locus_tag="PK252_04140"
     CDS             complement(56959..58425)
                     /gene="crtI"
                     /locus_tag="PK252_04140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT13932.1"
                     /translation="MTKTACIIGSGIGGMAVAIRLAAKGYKVKVFEKNDMPGGRISQA
                     EENGFRFDTGPTKLYMPEMLDELFVLHNKNPRNYFNYHRAESSGLYFFPDGTQINAWC
                     NAEKLADEISNKTSVPKHKVYQYLHKCQKLWELASPLFLYSPLYLCKNFLKAPCRKAL
                     LQFYRLDIFTSLDKRNRKLFLDSKVVEIFNSAASSFGNNPYKSVATIKYVSHLNYNLG
                     VYHVQNGMYSIVKALYDLAIEAGVTFYFDSPITRVFQSNKRIVSINTATNAYFADLLV
                     IDTDVVHFYRDLMQDKKMAKKAMKKKLTSSAIIFYWGLSKKIEGLNFYNIIVSGDSKK
                     QFDTLFKNKAIDSDPSIYINDISGLSGNNNPEGYQYLQVMVSVPAKHGHNWKKVTEEI
                     KNIVLSKIEKTFGENISKNILFEQVATPLTYTNIYNTHNGSLFGQAINSFRSFFTKHP
                     NFISRETENLYFTGSSVHPGGGIHHCLASAKIIATEAN"
     gene            complement(58488..59264)
                     /locus_tag="PK252_04145"
     CDS             complement(58488..59264)
                     /locus_tag="PK252_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT13933.1"
                     /translation="MPTFLFDKIIFGPVKSRRLGHSLGVNLLPTDAKVCNFNCIYCEC
                     GLNTKGKKDLYLPTVGEISNLLQRRLNEMKSKGEPVDTITFAGNGEPTMHPQFEEVID
                     AVITVRNNIFPQVKIAVLSNSLLLHKQSVLNALKKADLNIMKLDSAINRTFQILNQPQ
                     LPITANDVIENLKKFNGKLVVQTMFVKGSFNGQAFDNTTDTEIIAWTDALKSISPELV
                     MIYTIARDTPVETIEKISGKQLKAIASTIEKAGLRVSVSE"
     gene            complement(59443..60012)
                     /gene="efp"
                     /locus_tag="PK252_04150"
     CDS             complement(59443..60012)
                     /gene="efp"
                     /locus_tag="PK252_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT13934.1"
                     /translation="MASTADFKNGLCILFNNDIYTIIQFQHVKPGKGGAFVRTKLKSL
                     TNGKIIENTFTAGEKVDLAHVERRPHSYLFSDEYGYTFMHNETYEQVSIPKNMIENAD
                     LMKEGQYVEIVFDADNETPLSCELPPFVVLEVTYTEPGLKGDTASSTALKPATVETGA
                     RINVPLFVNQGEKIKIDTRDYSYCERVKE"
     gene            complement(60427..61116)
                     /gene="radC"
                     /locus_tag="PK252_04155"
     CDS             complement(60427..61116)
                     /gene="radC"
                     /locus_tag="PK252_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT13935.1"
                     /translation="MKPNNSIKNWAEDDRPREKMMRKGIGTLSNAELLAILFSTGSRE
                     MSAVELARQLLEMADNNLDRLAKMGLDQLMQIKGIGEAKAISVHTALELGKRRSVQPY
                     EEAKTFASSHDVYTYFGNKLRDLPYEEFWILLLNRANRMIEPVKISQGGMSGTVVDVR
                     IVLRNAIIKQASSVIMCHNHPSGNTKPSQQDIDITKKVKEAGALMDIKLFDHLIVTDK
                     AYFSFADEGMI"
     gene            complement(61260..61913)
                     /locus_tag="PK252_04160"
     CDS             complement(61260..61913)
                     /locus_tag="PK252_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT13936.1"
                     /translation="MIHPIKVFIVEDHKIFREGLKLILNTVKNVKVIGEAPNGKVFLE
                     LLNESMPDIIFMDLNMKEMSGIEATEKALELFPELKIIALTSFEDEEYLIKMADLGVK
                     GYLLKNSLRDDFIKAIERVMEGYTYYSDELIVKLSKRFNEIQKKRHQQPEETFSDEEK
                     ELLTYICQGMTNKQIADLMHLSSRTIEAHRARLLEKTQTKNSVALAVYAVNHKLVEL"
     gene            complement(62043..>63191)
                     /locus_tag="PK252_04165"
     CDS             complement(62043..>63191)
                     /locus_tag="PK252_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13937.1"
                     /translation="IILTDSLNTEKSFGSFINTGIHALPADMEKKHALHESTGGVNIL
                     WQNEYIVLGALGTATKYDAVKPASDDFYKRFAFTGNSISNASTYYRVNTRRIRFYGET
                     AMAKNYSLALLHGIAVNPASEICFSLMYRYYDKSYYAPYANAFGQQSLNVNEHGWYAG
                     IDIQPFTKYFVSLFADITSYPWLKYRTSEPSYSSTYYFQVKRIFDNGLNLSARYTAKY
                     GMQDSSGTIPVKHIIDYTTQRARLEAKYSPNEWISITNRAEWVWYNEDGQSKQSGFAM
                     LQDICWKPMQERFYLQGRLAWFNTDSYDARIYAIERGAGSYMSIPVYYGKGLRYVVSG
                     KLKLSKRMNAQIHYSSTHYFDRTTLGQGGYAIDGSDKNEIVVACKLRL"
BASE COUNT        19320 a        11896 c        12052 g        19923 t
ORIGIN      
        1 aataactatt tgaatatctt tgttttataa aatcaaaccg tcaaagtcgg gaaaatcagg
       61 aaatcaaagg tataaggaag cgctcatcga ggttattttg aagtctgtaa actaacgtga
      121 attcgatata agaataacat tgccaattat tgttaaaaag gcaataaata caaagctttt
      181 aataataaaa cagggaaaca ctgttggagg atgcccaaat gatgcgcaac tgccagatta
      241 tataaggcaa acattagata caggctcgta agcgcttgac aataaagcag tttgcaaaag
      301 acaaaatact ggtttcgtcc attgattggc tcgacaggct gaagtttaaa ataagactga
      361 tagaatttga ggctatagca gccctgtaaa caaacattgc aactatccgg ataaatagaa
      421 aattcggata gttgcattac tattctttta ccaccagctg agaataaacc ttgtttgttg
      481 actttaactt aagcaaatat aggccaggct taacattaaa ggtttctatt tttactaatc
      541 cccccgatac agctttacta atgcatgttt ttccgtcaat gtctattatt tcgacatgat
      601 taattttcag gtcccaattt tctatcatca aataattggc cattggatta ggaaaaactt
      661 tgaacttcga ttgttttaca ttctctacac cagtaatatc aatagttttg caaagagagt
      721 tattgtttac attggtttcc aattttgaat taggcgccaa acattgaaaa catagcttat
      781 tgttatttag cccgtcctca tatttgtgat ggatttttac tgtttgagtc tgaccaggaa
      841 gaatattaag gttttggtat tttccataat aataattttg ggcacaattc atacctccat
      901 gtaattgaga aaaaatggaa agacccttaa ttgtatcatt accgttgttt ttaatatcaa
      961 actcagcatt gaaataatac cctcttggaa aagagtctcc cagatatttt acataatcaa
     1021 tgcaaatgct atccacctta aaatcaacta attctatatc aggcaaagta taatcttctg
     1081 aagcaaaagt atcagcgtga aaatgataaa tacctatttg attggtaaac gaattacctg
     1141 caaaaacata atttttcccg gctgcaataa acctgatatc gtttgctaac aacggaaatg
     1201 tcaatgtatc tatgcttttg aaatcattag tttttataat ctttatgctt tctgcttgtt
     1261 tcaattgcag ccaaaaagta ttgtcaaaag tttcaatgtt ctgaaagcct aaagtgcttg
     1321 aaataacagt atctataatg tttaagcttg tatcaaggcg cacaagacat gagcttagca
     1381 aagtataaat ggtatccttt tttaaaaatg cttttactat gttgccagaa aatgcaatgg
     1441 aatcggatag aacaaacgaa ctatccgatt tgtatattct ttccggagta ttgattagat
     1501 aaaaacgggg atttatcggg ttaatcccgg tgatgtccat atttgagcca aaatcatact
     1561 tttttattcc agatccgtta atgtcagatt taacaacgga tgtttttgaa cagaccagaa
     1621 tttttccatc atcggtaata tttattcctg tgggatagaa tccaaaatag ctgtaattag
     1681 atgttttttc agaataatta agtctgaaaa tctctgtacc ctctttgttg tatttggaca
     1741 aaaccaattt ttcatctccc acaatatcgt ctgtagcaat agtgtaccct gttgtataaa
     1801 tgttatcttt atccgtgaca attaaatcac taacactata cctgctccaa agcttttgac
     1861 cagaaaaatt ataggcaaat aaattctgac cgctgcacga aacatcacta acaaacccgc
     1921 ttaccaatat catggaatct tgaaacaatg ccacatcggt aatatgcgat ttgttatgac
     1981 aactttcatc atcaacaatg ctccaaaagg actgggcatg caaaacaatt ggcaataatg
     2041 ccaaaacaga aatgatcaac ctcttcatat ccatttattt tagaaacgaa gaattaataa
     2101 tcttttgtct gtaaataaaa tagcgttttt acttggccaa aaactttagt agttcataaa
     2161 ccaaaaatgc cggccatacc aaagctttta aaaagccaag tacacctgac caaaagcttg
     2221 tggcctgcga aatataaaat atggcagcgc caatcatccc caatccataa atagcccctg
     2281 aaggcccgtt gtttgttatt ctttttccca tagcattgac ttttagtaat ttaataacga
     2341 aaggtataaa aaaaacagtt atcaaataga caaaggggaa ctaatatttt tctgagggaa
     2401 agagtttttg ttttgtcagc cacatttgca tttttaaccc attgacagat agtgtttttt
     2461 tcgttgataa taacattatc atttgcgaaa attaatataa aatgttaact ttaaaataat
     2521 tgaaaatact tctatgaggg ttattccaaa atcaatatgg agctttcttt ttgaggaaac
     2581 aaagtacagg cttcccatac ttatttcagt gtttgccctt tttattggtt tgtttgtttt
     2641 ctttcatggc aaaatagact acatcgtcaa tctttacttt gggataccgg taattatggc
     2701 tacctattac tacgggatga ctattggtgt tgggcttttt gctactgagg cgctggtaca
     2761 tgcaattata ttgacattta agaatgaaga catttcgttt ttttattcta accatcgtgt
     2821 tgttattgca tttataagcc tttttgttgt ggtatatatc gtttcgctgt tcagaaaagt
     2881 cagcaacagg ctgcttctta gcgaaaaaaa gaataaagaa gtgtctgatg aactgcaaaa
     2941 gctgaacaat tcgaaagaca tgtttttttc gcttattgcc cacgatttgc gcagccctat
     3001 tggttctatc aggtcgcaaa tagaaatgtt gctcgataac aagatacaat tgactgaaga
     3061 tgagcggaca aaagtattac aagccttaca atctttggcc aaccgggttt atagcttgtt
     3121 agaaaatctg cttaaatggt cacaaataca aaaagggctt ttgagcatta cattagagcg
     3181 tattaacatc caaaacatct gtaatgactg tattttactg tttcagcaag cagccaaaga
     3241 caaagaaata agcatttata ataaaattac gagcgaactc tacattatgg ctgacaaaaa
     3301 tgcaatagaa actgttatcc gcaacctgct tgctaatgct ataaagtatt gtaccccggg
     3361 cggttgggtt tcagtcagtg ctattgaaaa ggccgataca tattgcatca tggtaaccga
     3421 caacggcatt ggcatggaaa aagaactggt cgaaaatttg tttaaaatag atgtcgatgt
     3481 aagccgcaaa ggcactaaag gagaaaaagg cacaggactg ggactaatcc ttgcccatga
     3541 actggtggac agatgtcacg gaacacttac ggtagaaagc cagcccacca taggcagcgt
     3601 gtttaaaata gagctgccta aagcagaatg acagatatta ttagtttttt accaaatcct
     3661 ttttgtttac cttaacaatc tttccaaggc gggacaagtt attcatgtta aagctccggg
     3721 tatctccata aattgtaata attgtaggct tccctgacac attttgacga tagaagtcaa
     3781 caatatcttc aaatgtaaaa gagctgaagt agttatactg gtcttttcgc atgtcttgct
     3841 tatacccggc attgataaat ttttcaacca actcggaacg tcttctgaaa tcaggttctt
     3901 tcaaacttaa tgattgtaaa aggtttgttt ttattatatt gtaacgttca ggttttatgg
     3961 gcatcgagtc tatgagcaaa taaaaagtag ccaaagcttc ttcagtttta tcactctgag
     4021 tagcaatatg gctaaacaag tatccgggtt tttcggggta tgaaaggtta taatcaaaaa
     4081 aactgtaggc cgaataagca taagaacgct tttcgcgaag ttcctgaaaa actatagaac
     4141 tcatctcggt gccaaaataa ctgttgaaag ctgaagaaag gtagtattgg tctttattta
     4201 acacgttgcc ctcgcgaaat atatatattt tgctttgtac agcttttttg tcatttacaa
     4261 taaagatagt attttgggtt acaacattcc tgtctttata taccggagat ttaaaagcta
     4321 tcagttgaga gttaaactgt atatcttttg caatggcttt tttcaccgtc tctgcatcta
     4381 atcgtcccga ataatgaatg gttgcctgat atttcattgc ttcgtgaaaa gaagccagta
     4441 attgtgccga ggttagtttt ttaatttctt ttaaagaaag gcggttcagg tagtccgatt
     4501 ttttgccata aagcacatat tgctgcaatg cccatcccat aaaatcaggg cttgaagttt
     4561 caaactttct gtccaactta gcttcatcaa ggcatttcat aagctgtttt tcatcagcct
     4621 taggttttgt tatcaattca ttggccaaaa gcagtgtttg tccgaggttt tcgtcaaaac
     4681 ctgtaatatt aatgcatagc gcatcttcat cagcccaaat tttaatatca gaccctaact
     4741 tttgtaattc ttttctgaaa atatcaaaat tcatgttttc agtaccaata aggttcatat
     4801 agtttgcggc cactgccagg gttttatcat aataaacacc gcgttggaat ttgatgttga
     4861 tgttgaaaat attgttgtac gggttgttga catgaaaaaa tgccaggttg gattgcagag
     4921 agtctactac aacatccttg ttcagctcaa taaagtgtgg ctgaggaata ataacttctt
     4981 tgttgtagat ttcttttgca aaaactgatg ctttttcacc ttcttttgat tctattggtt
     5041 tgtaagcagg cttttcgagt ttagtctttt tagggaaccc ggttttggag tatataacaa
     5101 gatggtcgtc tccaaaatat ttatttgcaa tgaggacaat agttttcttt tttaaagtat
     5161 caataatctt gtcttcttca aattttattt tgtatgaaac gccatcaata aaagcatcta
     5221 tgagcatgtt tgccctgcca tgtatatttt ctttgttgtt taaaagctgt ctgcgttttt
     5281 cagttttaag cgaagaaagc agttcgtccg ggaaatctcc gtttttgaca cgttgaattt
     5341 ctttctttac cagcgcctca gcatttggca atttctggcc caatattttc ggtacaaaga
     5401 aaataatatc agcgccagca tcagggaatg acacaggaga taaaccggcc ataagtatct
     5461 tattgtccat ccttaacttg tcgagcaagc cggttgacga gttgttggat aataataagt
     5521 taatcaaatc aatacttatg ttatccggat tggccgactc gggggtatga taactcaaca
     5581 atccaacttt tatggggaac agcttatatt gataaaattc ccttccggtg aaacctttag
     5641 gtttaggaag tttattgaaa tttttctcgc ctgaacgtag tttcccaaaa tattgctcaa
     5701 tccaaacctt tgctttgtca gggtcaaaat cgccacaaag taccaaagcc atattgtttg
     5761 ccacgtaata cttgtcaaaa aactcataca ttttcgataa gctgggattc tttaaatgtt
     5821 cgctcaaacc taagaccgta tggtggccgg caggatgccc cgcaaaagcc tttgaaaaca
     5881 aggtgtcgat aataatcgaa tacggatcgt caaggctcat attcttttct tcatacactg
     5941 tttctaactc agactgaaat aacctaaaaa caggctttct aaatctctca taatatatat
     6001 gaagccaatt ttcgagctgt gacgatggga atgagttgtg atataccgta cggtcaaagc
     6061 ttgtataagc attcacttct gtacctccgc aatgtttgag gattttatca gtttcatttg
     6121 ggatggcata ttctgccgct tttaccgaga gttggttaat ttgttgctga atttcttttc
     6181 gctttttttc atctttggta ttgcccagtt catcgtattt caaacgaata ctatcgagca
     6241 aaacactttc ttttgcccag tcaatagtcc ctatactgtc tgttccttta aacataatat
     6301 gttcgaagta atgagcaatg ccagttgctt cattagactc gttattagag ccggcacgta
     6361 ccacaacagc cccaaatatc ttaggcaggt tatggtcggg gtttaaatac accgtcaggc
     6421 cattgcttaa cgtatattgg tcaacattat agacagatga gccggtattt gttgcaaaag
     6481 aagtgttaca aaaaactaat aatgcaaaga tgggaatgat aaaatttttt ctcatgtaaa
     6541 agggttttgt taaaaaaagg tttcaagttt caaacttagg gttaattttc aaaaaaaaag
     6601 ggaaaattga cataattatt gttaaatgct aatgggtcga aacagatgag agaagcctgt
     6661 ttaatttgta attgtatctg catgtcaggt gttgtaacag attatttact aattttacac
     6721 actttaaaag aaaatggcag aatattttgc acatccgacc gctgtaattg accaaggttg
     6781 caccattggc tcagggacta aagtgtggca cttttgccac ctgatgcccg gctgtgtaat
     6841 tggcaataat tgtatcctcg gacaaaacgt gatggttgcc cccggtgtca ggctgggcaa
     6901 taatgttaag gtacaaaata acgtatcatt atattctggc gttgtttgtg aagacgatgt
     6961 attcattggc ccttcggcgg tgtttacgaa tgtcatcaat cctcgcagtc acgttaaccg
     7021 taaagaagaa tttaagcaaa ccattgtagg taaaggggct tccattggag caaatgctac
     7081 tattgtttgc ggagtaaata ttggccaata tgcatttgtt ggggcaggtg ctgttgttac
     7141 taaagacgtg ttgccatatt cgttggtaaa ggggaatcct gctcgtcatg caggctggta
     7201 tagcgaaaac ggccataaac ttacatttga taataaaaat attgctgttt gccctgaaac
     7261 cgggcaaaag tacaaattgg aaaagaataa tctgaaaaaa ctctaaatca taattgaaat
     7321 ggctaaaaaa gctttaataa ccgggattac agggcaagat ggttcgtacc ttgccgagtt
     7381 gctcctttca aagggatacg aagtgcatgg catcatccgt cgtgcttcat cttttaacac
     7441 aggccgtatc gaccatttgt acaaagaccg tcataacaaa gatgtacaat tgtttttgca
     7501 ccatggagat gtaacagatt cgagtaatct caaccgtttg ctcgaaaaaa tccatccgga
     7561 tgaaatatat aacctggctg cacaaagcca tgtaaaggtt tcttttgatg tccctgaata
     7621 tactgccgag gtggatgcta tcgggacttt gcgttttctc gatgctatta aggaaatagg
     7681 cataaaaacc aaattctatc aggcttctac cagcgagctt tatggcaaag tccaagaggt
     7741 ccctcaaact gaaaaaacac cattttaccc ccgcagccct tatgctgttg caaagcttta
     7801 cggatactgg attattgtta actaccgcga agcatacaac atctttgcct gcaatggcat
     7861 acttttcaac cacgaatcgc ctcgcagggg cgaaacattt gtaacacgta agattactat
     7921 tgctgctgcc aaaataaaag cagggcttca ggacaaacta tacttaggaa accttgactc
     7981 acagcgcgac tggggttatg ctcctgaata tgttgaaggt atgtggcgca ttttacaaca
     8041 tgacaagcct gaggactttg tattggctac aaaccaaatg catactgtcc gtgaatttac
     8101 cgaattggcg tttagtcact taggcattga acttgaatgg cagggaaaag gcgtaaatga
     8161 aaaaggtatt tgcaaaaaaa ccggtaaaac tattgttgaa gttgaccctg cttatttcag
     8221 gccaactgaa gttgagcagt tgctgggcga ttattctaaa gctaaaaaat tgctggggtg
     8281 ggaacctaag gttactttta aagaattggt aaaaataatg gtcgaagctg attacaatac
     8341 ttatggaaaa aaactctaaa atttatgttg ccggccatct tggccttgta ggaagcgccc
     8401 tggtacgtcg cttacgcgaa aaaggatata ccaatatcat tggccgctct attgatgaac
     8461 ttaatttgtt ggatcaacga gacacattaa agttttttga aacagaaaaa cctgagtatg
     8521 tattgttggc agcagctaaa gttggcggta ttgttgccaa caacacttac cgtgcccagt
     8581 ttatatacga aaacctgcaa atacagaata acattattca tgcttcatat ttgacaggcg
     8641 ttaaaaaact tctttttctt gggagctcct gtatttaccc taagcatgcc cctcagcctt
     8701 tgcgcgaaga ctttttgctt acttctgacc tcgaatatac caacgagcct tatgctttgg
     8761 ccaaaatagc aggtatcaag atgattgagt catacaacat ccagtatggc accaactaca
     8821 tatcggtaat gcctaccaac ctttatggcc ccaatgataa ttacgacctc gaaaaatcgc
     8881 atgtactgcc tgccctgata cgtaagatgc acctggcaaa aatgttgcaa aatggcaact
     8941 gggaagctat ttgcaacgac ttcaacaaac gccctgtgga aaaagtggat ggtaaggctg
     9001 caaaagagac tatccttgct atgctcaata aatatggtat ttctattgat acatcgggca
     9061 aagctactgt aaaactatgg ggtacaggta ctccccgccg cgaattcctt ttttcagatg
     9121 atatggccga tgcttgcgtt tatctgatgg aaaatgttga ctttaaggat atactggaaa
     9181 aacaaaacct tactggtggt gaaataaaaa atacccacat caacattggc acaggccgcg
     9241 acctcaccat caaagagctt gcttacaaag taaaaagcat tattggcttt gaaggccaga
     9301 ttgattggga tgccacaaaa cctgatggca cagcccaaaa acttttggat gtttcaaaaa
     9361 ttcactctct tggctggaaa gaaaaggttt cgatggacga aggaatagct aaggtttata
     9421 aacagtacca ggagtaaatt ttatatgtac gaattcagct aagattctgt cttagctgtt
     9481 tttaatgtca tttgaattat tgtagcattt actaagcttt aagaaccgct taaaaaggtt
     9541 ataatataaa acaaagatta tggacaacaa caaactcatc caagtcgcag ccggatgcgg
     9601 gctacattgc ggggcttgtt cactatatat tggtagtaca gaggcgcctg aaaggttaag
     9661 aattttatca gaacgaatgg gacaaactca cttgttatgg ctgtagaagt gagaaaaagt
     9721 ctgttttttg tcaaagttgt aactttatta aatgtttgga tcaaaaagga ctagattatt
     9781 gtggacaatg cagcgattat ccttgcgatg aattaaaagc gtttcagaag gaaaggccac
     9841 atcgtattaa tctttgggat gatatgaaat gtcttttaga aaaaggcatt gaaaaatggg
     9901 attctgaagt tatggataaa tataaatgtc ctacttgtgg caccataaac tcttatgatc
     9961 tcgtgtgccg caaatgtggc ctaaaaccat cttgcaaatt tgttgagacc aattatcagc
    10021 aaattgaggc tgctatgaaa taatatttag gcaagtaagg cttataataa gtcttttaaa
    10081 aagaaagtac ttacaaacag cttgaattgt atatttttaa accacactat cctgtttact
    10141 tatgttcaat gtggcgctta taaagcaatg taatgacaat gccaattatt ccaattaggc
    10201 acaatactcc aaaaaaatat aatgcaatca ttttctttaa tttttaattg aaagaagtaa
    10261 aaaaccccaa atagcaaata agagtgtagc caatactcca actatcaaaa ctaatagttt
    10321 attgtggcta attttcagta cgacacttaa tacaactcct ccaaaagtca attttgaaac
    10381 atcaatcaga tattccccca attatgtggt catatttttc ttttcatcca tgttacaaat
    10441 ttacatcgtt ttaaaaacaa ttcctaaact ttgtcaatgt gccaaactat gtgttgctta
    10501 taattttgat gaagtttact ttgtaatact gccctttatt tttccccttt caataccttg
    10561 aaaagctcaa ctgtcttctt attatcctta aagctgatat aaatcgggta ttcgttctta
    10621 gcccggataa gcatataagg ccttgagccg aggtgtacag atagttttac ggtgttacca
    10681 tcggtaagac ggaaatagcc tttcaggcgg ttgccaaaag catagccatt ggtgcgcatg
    10741 gaaataggcg gaataacttg aactgtatct atactggata tatctgcaat attaacttta
    10801 tacccataca ttccgctcag ttcaaaaaca ttgttttcaa attttgcttt aggttccttg
    10861 ttcccccaaa ggataaacat ggtaacgata agcaaagcgc tggctaaaat tgcccagtaa
    10921 agcaaatatc ttgaatttgt tggtatttcg gcgttggttt ggtgtaaatc attgttcaat
    10981 ttggagttga tatagttact gacatttacc ggctggtctg tgccaatgcg taccaccgat
    11041 ttgcggtttt tgaatttcac ttcaatggca tctaagcccg aaacattata aagccagcca
    11101 ttgggcagca tctttatgcc tatcccatgt ataaaagaat tgcgcacggg cttaactgac
    11161 tctatttcgg aaagcagata tgacttgctg atcaatccga aacctaagct aaaagaaagc
    11221 cgttcatctg ttaccgtaat ggtaagttta tgaaataagc caaggcaaac gacaaataaa
    11281 agtacagaag gcaataaaac atattttgac gggtcgtcaa aaccaatata aaagagaatc
    11341 cccgtaaaaa gcaaaataaa cgacccaaaa agcacaatca taaaagtgcc tcgttgggtg
    11401 aatgttttct ggtccatgat ttgtatagtt tatgtgataa aagtaatttt tagttcatta
    11461 aaacaaaata tgatgcaatt atttttgtat aaatttttta gtggtttaag gtgcagatgt
    11521 tgtcagttta ccgggaatat tgtctgtttt accatttttt agcttgaaat gaaattatat
    11581 gcattacatt tacgccctgt ttgcctttta ccattacatg aaccaatgtt tgcataaaaa
    11641 actatatact ttcattgtat ttgtttgctt gtcttctttg ctatcggggc aaatgacccg
    11701 tataaaaggc atagtgcgtg atgccgaaac tggcgagcct attccttatg cctctgttta
    11761 tgtcaaaaat acccctttgg ttgtatttac taatgattct ggggcttatg tatttgaaac
    11821 aaggcagaaa attaatgcta tttcggtttc atttttaggg tacaaagaag ccaccgaaga
    11881 agtggacaaa ggcattacac aaatcatcaa ctttgatttg gagcccaacg ttacggtgtt
    11941 tgaagatgta gtggttcgcc ctggtgagaa ccctgctcac cgtattttga gggctattga
    12001 taaaaacaga aatcgaaaca gtttaagatt gaataatatt cagcgcaatc agtacagcaa
    12061 aatacagatt tcgttgaaca atattgatga aaagtttaaa aaccgaaaaa tcctccaacc
    12121 tttcaagttt gtttttgaca atgtggacac caatgccatc acgggcaatg tgtatttgcc
    12181 aattatgatt gccgaatctt ctattgacca ttattcacaa aacgaccctt cgtttaagaa
    12241 agacattgtc agggctacca aaatttctgg tatccaggac gaaactatta tcaacttttt
    12301 tggtgggctc aaccaaagct ttgatgttta ctccagttat atggatttat ataccgagtc
    12361 gggctttatc agccctattg cagataatgg gcttttgttt tacaagtatt atctgctcga
    12421 tagcgctgtc agggccgggc acaaatgcta ccacattagt tttaagcccc ggcgtaagca
    12481 agaacgtaca ttttttggcg atttctgggt agccgactct tcttttgctt tgcagtcaat
    12541 gactatgcgc ctcaaccctc aggcaaatgt caactttatt accgatttgt atgccgaata
    12601 ttctttttat cccgaaaatg attcgttgtg gcctatacgg agtgaattta ttcagatgga
    12661 ctttaacctg gccgaaatga aacgtgttaa aggggtacag ggaaaaaaga cagttatata
    12721 ctccaattat aaaatagaca agcccattcc aaaagaaata tttgatgttt caaaggatgc
    12781 cgagaagctt gattctttga ccaacagctc tcaaatagat actttaaggc ctgtacaact
    12841 ttcaaaatcg gaacaaacga tttatggaat ggtggactct atcaaaaacg tgccagcctt
    12901 taaaactacg tataacacca tccgtaccat tgttgaagcc cattattctt tcgagaaatt
    12961 taaaattggc ccatattttt cgttatatag ctacaacagg gtagaaggac accgtttcag
    13021 gattggtggg gttactaacc aaaatttcag caaacgcatg agggccaacg gctatgttgc
    13081 ttatggcact gacgataagg aatacaagta ttttgccaac atgcactatg ttgtttccag
    13141 aatcccctat acgcgcatta cagcgctgta ccgccacgat atgagccaga tataccttgc
    13201 cccgggcgaa ttgctcaacg aaaacattgt gtcgtctttt atccgccgct atgactttaa
    13261 aaagctgcaa atgatagaca acgccagctt taacatcgaa agtgacatag ccagttcatt
    13321 gtccgttatt ttgggaacaa actactttaa ggttgatgcc ggcaaatata ttccatttgt
    13381 aaagcccgat tcatcagagg tgaaatcaat taataccggt gaaattttta ctaacattca
    13441 ctttgaaata gggcagaagt tttttaccac aaaatataag cgttttagga tgcaaaacga
    13501 aatgcctgct tttgacctga acctgaccgc aggtattaag gggatgtaca aaggcgagta
    13561 tgactacctg aaaacttcac ttacatatag ccaatacctg aaaaccaacc ctttcgggta
    13621 taacaaatat tcggtagaag ttggcaagat atttggcaaa gtgccatggc cgttgcttaa
    13681 tgtgtttaaa ggaagcgaaa cttacgggtt tgagcccaac gggtttaaca tgatgaacta
    13741 ttatgagttt gtagccaatg aatatcttaa aataatctcg gagcaacact ttcaaggcat
    13801 atttttcaac tatgtcccat tgttgcgaaa gctgaaactt cgcgaagtgg ctacatgtaa
    13861 ggctgttatc gggcacctca actataagcc agagatgatg ctgctgccct catacatgta
    13921 cgggctgcac cagccttatg ttgaattggg tgtgggggtc gaaaatatct tcaaattttt
    13981 ccgaatagat gccttgtggc gccttagcta ctggaataac cctgatatag agaagtttgg
    14041 catcaggcta agcttacagt ttattttgta atcagtttta aattcctgtt ttagctcttt
    14101 tacccaggta aaaaaccatt tcgccgtttt tatgtttaag gaaagtacaa taatagcaac
    14161 ctttgcctcg aaataaattc tttttacaaa catgaaaagc aaaaggctta tgatcatatt
    14221 tcttttatca gtgccttcat tgataataat tggcatatta tttatgcagc aaaaggcttt
    14281 tggacaacaa cccaaagaag agcgccttca aaaaatcctg ctctcgccta attataaaga
    14341 cggcaaattc caaaatatgg aagaaacacc tgttatgagc gaagaaggta tgacttataa
    14401 atttttattt gaaatgttca aaagacacaa aaatactaag ccatctctgc ctattccttc
    14461 tgtaaaggtt gacttaaaac aattagttgg cggcagcact gatatagtat ggtttgggca
    14521 ctcttcatat ctgatcagga caaataaaac aaacatattg gttgacccga tatttagtaa
    14581 aaaggcatca cccgtgtctt ttgccggggt aagcagtttt gaaggttcat tgccttatgc
    14641 tgctgccgat atgcccgata tcgacattat ggttatcacc cacgaccatt acgaccacat
    14701 ggactataag acaatcatgg cattgaaaga ccgtgtaaaa ttctttgttt gtccgttagg
    14761 ggcagggcaa catctggaca aatggggtat tgctaaaagc aaaatcattg aactcgactg
    14821 gtgggaaggc aaagaaatag ttcccggtat catgcttaca gctactcctg cccgtcactt
    14881 ttcaggccgt ggacttgtcc gtaacaaaac gctttgggct tcatttgtgc tgaaaactga
    14941 tattttgcag atatttattg gcggcgactc aggatatggc aagcatttta aggctattgg
    15001 cgaaaagttt ggggcatttg acatagcaat gctcgaatgt gggcagtaca acaaattctg
    15061 gccatacata cacagtatgc ctgaagagac tgcattagct gctaaagacc ttaatacaaa
    15121 ggtgctaatg cctgtacatt ggggaaaatt taaacttgcc atgcatccct ggtacgaacc
    15181 tgttatgcgt gttggggcca aggctgccga acttcatata cctattgtta cgcctgttat
    15241 tgggaagcct tttattcttg aaaatattgt gaatgaacca ctttgggtaa atgaattgtt
    15301 gaaaaacgat aataatgtga cgttaaatta aggattcttc ttgttcgcac ttttcttgag
    15361 ctctttttgt tcttggggaa acaaggtaaa gccatatttc tgtgccactt tttcgatggc
    15421 aataatggca aagctcgatc caggaatagc ttgcaggcct atcaacgcca gagcctttcc
    15481 caggtcaact gattggcctt tcaggaattg tacttcttcg ggtgtcacat cttttccttt
    15541 caacatcttc aatagaatct ttgatgcaat cttggtttcc tctgactccc gtttaaaagc
    15601 cctcccaaca ctctttatcc agctccaggt aacaatggct ttggttaaaa tgaagatttt
    15661 tagtttttga agaagttggg acaattgaaa aatcataaat gagttaattt gtcaacattt
    15721 ccattgagaa tgtggggaag attgctttta attttatctt cgtcccagtt ccaccattgt
    15781 atatctaata gctttttaat tacctgttca gaaaagcgtt tttttatcac ttttgctggt
    15841 atcccgccta cgattgaata aggaggtaca tcctttgtga ccactgcccg tgttgcaact
    15901 attgccccgt ctcctatttg tacaccctgc attattactg cttcgtaacc tatccaaaca
    15961 tcatttccaa taataatatc gccttcatta tcccatgcat ctgttacttt attacgttcc
    16021 aaaccccatt cttcccaaaa aagagggaat ggataagtgg aaagtgatga catcctatga
    16081 tttcctgatg taaacatgaa ttttgcccca caagcaatgg aacaatattt cccaattttc
    16141 aacttgtcgt tatttattgg ataatggtat aaaacattat tgttttcaaa ttgggttggg
    16201 tcatttacaa aatcattata catcgtgtat tctccgacta tgatattggg ctttgtaatt
    16261 gtaggcctca gatatacggt ttctttatca ccctttctgg gatatacttc gttactattc
    16321 atttccagat tttaaagatt attctatact tatcagaaat acggtattaa cttttgtata
    16381 tttcaaataa cagccctaaa atccttattt tctcgtttat cttattataa actgagtagc
    16441 aagatgccga aagaaaaaac aaaccagaaa aaattagggt aaaattgtta aactttgaaa
    16501 cactgatatt gttctgaaag ctaatcaatg caaaaataag aattaatgta catgctgata
    16561 taaatataaa ataagcatat ttcgttcttt ttgattctcg tttcagtttc tgaaactcgc
    16621 tatgccaatc aatattggcc agtttagttt cagttgcttc tattgataat ccgcaaagct
    16681 ttgaatactg tgcaatcaac atttctttaa ttttatcaat cttggggttc atagattagt
    16741 ttttataaag ggtttaacgt aaaaaaattc gtcaggagtt tcatctgtaa atagtaatga
    16801 aatgtgtcgg ccttcgtggt tgatttcatt tttcatcgaa acagtttaat ataattatca
    16861 aaaacgtaca tacgacttct ttgtccgcca gtcacctctt ttagaatatc cattttttct
    16921 aaatcggaaa ttaacttgta tgatgaaggc attgaaatat cggctatttc atggattttg
    16981 tctgcattta aaactggccg tttatataaa taatcaacaa tttttttagc ctttgcggcc
    17041 ctgctaccta aactttgcaa gttaacttct gtcttttttt gcaattgaag tatattgtca
    17101 aaagtttcaa ttccattttt tgcagtttcg ataataccta ccaaaaaaaa cttataccat
    17161 tgatttagat cacttttatc ccttactatt gtgagattat catagtataa ggccctattt
    17221 ttctcaaaaa aatcggatag atatagaatg ggtttttgca gaatcccttt gcttactaag
    17281 tataatggaa ttaacaatct tcctaccctg ccatttccat caagaaaagg gtggattgtc
    17341 tcaaattgat agtgtactaa tcctatcttt aataattcgg gcaaaaatag ttcttcatta
    17401 tgtaaaaatt tttcaatatc actcattagc tcaggcacag aggtatgtac tggtggtata
    17461 aaaatagcat cattaattgt tgctcctcca atccagtttt gactaattcg aaattctccc
    17521 ggttgttttt tctcgcctct tactccttgt aaaagtactt tatgggtctc tcgaattaac
    17581 cttgaagaaa aaggcaattt ttctaattcc gaaatcgccc attccatagc ttttatatag
    17641 ttctgaacct cttcccaatc atctcttttt tccattggaa tatcttcttt atctagtaga
    17701 gcttcatcca tgtttgtttg tgttccctca attttactac tctgagtggc ttcttttaaa
    17761 acatgcatgc taataaataa atctatgttg ggaatgtatt tagaatacat atccaatctg
    17821 cctaattccc tatctgcctg gctcaacaat tgcattacct ccatattgtc aatatgcagt
    17881 tgcctattga taaattcagg ttgaaagctt ttgtaatatc cctgactgat atattgtcca
    17941 gatttaaaat ttttcactgt taaaatttta aacaaagtta tatcttattt aagattttga
    18001 caattttctt aaataagagt tttcttaact taagtttttt tgaaatatta aaaaaattca
    18061 gtttgagttc tctcgtctgt tgtcaacagc tttggtttat gaagcgggtc aggagtatgt
    18121 atttgtcctc catggcgatg ttacttgtga tgaaaattat tgcagattta tacgcttgta
    18181 cttcttaaat ctgccatgtt tcataagcgt tttggagtgc ttaaatattt cataaaaaag
    18241 tcagtccaat tcgtttgttt aatccttttt caaaaatcct tatcaaagta gataattgaa
    18301 tattcccttt ggcattctct atctttgaga tatagctctt ttttgttcca gctttaattg
    18361 ctaattgttc ttgtgtcatt ttagcttccc ttctggcttc tttcagcatc tcactaacaa
    18421 taaacatttg agcttgttct tcatatttat ttctgctctc tgtcccgagt tttccgtgtt
    18481 ctaactcaat caactcatca aaattcttaa ttttactata cttttccata attataattt
    18541 tttaagttga aaatattctt cttttaatcg catagctctt tctatctctt cttttggagt
    18601 tttttgtgtc ttttttttga aatccattta gcaaaatcac tagttgtcct ttatcaaaac
    18661 aacaaaaaat tcgatatatg tttgattggt attcaattct aatttcaaat aatccatcat
    18721 atccagttat tggtgcaaga aatttttctg gaactctatc aacctgttta atcaattcaa
    18781 gcacatattg aaccttagct ttaagcttaa tatctaagtc tttataaaag ttaataaaat
    18841 aatctttatg aaatatgatt tgccttgtca tggagcaaag ttatcttaaa agataacata
    18901 ttcaaaataa taataaagta gaaatctttg ttttggtttt aaaatatgac aataagcaag
    18961 tccctttcaa aatactaaat ctgcttataa aaagctcctt tataagtgtc tacaatgggg
    19021 atatacttgc tgcctatttt gatgcggtcg ttttctatgc tttctatttt tggaactatc
    19081 acccctctga gggttaaacc tttggagggg tttcagagta taaaaaagca aatctatttc
    19141 ttcttaaacc accttttttt aaagaacttc tttttaggct cagcatgctc ctcgtcaaca
    19201 acttcttcac ctgtcgaaaa gtgctttggt acagggattt tgaacacctg cttttccagg
    19261 aaggtttcta tctggcgaaa tttatgacgg tcagccctgt tgacaaaagt taacgccact
    19321 ccggtagtct cggcacgggc agtacggcct atgcggtgaa tgtagtcttc ggcatctggc
    19381 ggtacatcgt agttgagtac caacccaata cctgcaatat ctataccacg ggcaacaaca
    19441 tcggtgccca ctaatatctg caacttgcgg ttacggaaat caagcaaagc ctgttcacgt
    19501 tgtttttgct ccaggtctga gtggatagaa gctacgtcaa atcccaactt tagcagttca
    19561 cgttcgatga ttcttacggt attcttttta gaagaaaaaa taatggcgtt gatgtttttc
    19621 ttttccttca aaagctcttt gatgaggcct attttgaaat ggtcttctac aaaatatgca
    19681 gcttgcacaa taccttcagc aggttttgag atggcaaggc taatctgttc cggggcatcg
    19741 cccataatct tcttggccag tgccctgatt ttttcgggca tagtggccga aaaaagaagt
    19801 gtttgccttt ctttaggcaa atagcttata atttggacta tatcatcatg gaagcccata
    19861 tccagcatac ggtcagcttc atccaatatc aaatattgaa cattagatgt atcaatatat
    19921 cccatagcca ggtgggctat aagcctacct ggtgttgcta ttactatttc taccccttcg
    19981 gtcagggctt ttttctggat atcccacagt accccttcgc caccgccata aacactgatg
    20041 gagctcagtt ggcaaaagta ggtaaaagcc tctacctgtt ggtctatctg catggccaac
    20101 tcacgagtag gcacaatgat gaggcattta aacgtatcta aagatgcttt ttgctcaacc
    20161 agtttgtgga ttactggcaa caaatatgcc gcggttttgc ctgttcctgt ctgggcacag
    20221 gctataaggt ctttgccact aaggattacg ggtattgcct gttcctggat aggggttgga
    20281 ttgttaaaac ccatggcatc gaggccttgt tgcaaatccg aagtaaaatt aaaatcaata
    20341 aaattcaatt tctgcttttt aaattaaagc atcaaaatta cctaataata ttcaaaaacg
    20401 ctaacatact cagaaatata tacatgctgg catttattcc agtatgatgg ttttaaatta
    20461 cttttgtaaa gaatacagat ttttacctat ggaaagcaaa ttagtaaaaa gctttttgaa
    20521 cgaccgtttt gcagagcatg tcggaataaa gcttgttgaa attggcaatg gatacgcttt
    20581 ggccgaactg gctttgactg aaaaacacct caatggcctg gataagattc aggggggagt
    20641 tatttttacc ctggcagatt atgcttttgg ccttgcttcc aattcgcaag gctttattac
    20701 tttggggctc aatgtgaaca tctcatactt caaatcgccc acagggaaag ttattagggc
    20761 agaagcaaaa gaaataagtg cacaaaacaa gatatgtggt taccaggtaa acgttacaga
    20821 cgaagatggc tcgctggttg ccagcttcaa tgggctggga taccgaaaaa tactgaactg
    20881 attatctgtt ccggccttgt tgaataagcg gctttaaaag gtattccaag ccattgagct
    20941 ttatctcgta catggtttgc agctgcatgc caagtttccc ttcaggaaaa cctttttgtt
    21001 tgaaccaaac caggtaaggc tcgggcaaat tgcagagcaa tcggccggca tacttgccat
    21061 agggcatttt ggtatttacc agctcgagca acaattgcgg gtttacaggg ttgatgtctt
    21121 gcatagctct aatttttgag tgaagatacc ttttttatgt gaaacgaaaa gcttttttca
    21181 aaaaaacgct tttcggtaag cctggccaat ggtgatttgc atattgttga cgttcatgat
    21241 atctcctctt atcaagtcaa gaagattttt gatacagaaa tgataaccta acaataacaa
    21301 atgagcgaaa atgagcgaaa atgagctaat ttttaaatct gtttaattaa gagtctcagc
    21361 cagctttgta atttgttgcg aaagattgtc aagcatttca tatcttttaa tgtacataga
    21421 gcgtttgtgc gattttatat cctggatatt tttacgggta ggccttaatg gaatttcaaa
    21481 ccagtccttg tttatactcc ttccgccaat ttcgtcccaa aaattatcat aatcaaaagt
    21541 aatgttacgt ttccagggga aattgacagc atgtttttga ttggatatgt gaattgaatt
    21601 gccaatacca taaattgcac taaaccccat attccttatt aattcttgtg cagaataaat
    21661 gattaatgaa ttagggcgca atccatataa cgatttttgc attaccttaa aggcatcttg
    21721 aacagccttg tttttatggc cctggataca accaattaaa catacccatt gacctttcgt
    21781 tttttcttca attgaaaaaa ctactgatgt aattcttcct ccaagcttat cacactctaa
    21841 ccacagagct aattcgcctt cttttcggat agcataatta taacctaact ttataaatcc
    21901 ttcatattta ttatcaaatt tcaatttagc aacaacaatg ccatcttttt gagtaagaaa
    21961 ttgtctgaat acatctcctt tgctctctaa aaatctatag gtatcaagga ttacatttac
    22021 tttccgttct ttatcccatt taattgaaat gtatggcctg aatggcttga catataaacg
    22081 acgacgatgt ataaaaacat gcctaaactt agatgattta agaaaattaa accaagcgtt
    22141 tgcaaatccc gggtttaaaa gagtaaaata aacaatcttt aattgttgct ttcttatgta
    22201 tttttgaggc ttacctgaat atcccttttt ggcaaacctc caaccagtga ttagtgctga
    22261 aataaatgga gggttaataa aagttttcat taaaacgttt aatattaatt gtttaacaaa
    22321 tctttaaaat gataaaacgt aaccctaaga cagcttaaat gttactctcc ccccaattag
    22381 agcagaacac ttttgaagca gataaagtct ttatttggct gtaaactatt tgagccaggc
    22441 cgaaaggcag ggtaagtttt caaagccaga cttttttggg ttttttgata aaaaatacaa
    22501 ctgaatacca gactgttata tttttttcat cggattcagg ttaaaattga aaatagcctc
    22561 aagcttttgt tgggggctat tttcttttat tgtggggcta ttttgttata aatcttgcat
    22621 ttctgtattt tttaggtgat atttgacata cctaaccaaa aaaataatta tgcccggcaa
    22681 aaaagaaaga caaaactctt tttttctttt ccaaaagata actgttaagg cttttctttt
    22741 gtgtttgact tttgttttag aggcttatcc gatatttgcc aataaaaaat ttaacttcaa
    22801 acatctcacc atcgaaaatg gcctttcgca aagctgggta cgttgtattt tgcaagacaa
    22861 atatggattc atgtggtttg gcaccgacaa cgggctcaac aggtatgatg gctcctcttt
    22921 tgtagtttac atgaatgacc ccgccaatag taaatctctt tcgaatagtc ggattaatgc
    22981 gttgtacgaa gataaagacg gcaatatttg ggtaggtaca ctcaatggct taaacttgtt
    23041 tgaccgcaaa accaacactt tcagttgcga tccaagctgg ccttcttcca gggttacttc
    23101 tatagctgca gacaagcaag aaaatttatg gattggtacc cactccggct tgtatttttt
    23161 agacaagcat acccaaaaag cttcttattg tgcttatgcc acagacaaaa acaaggtgaa
    23221 taatttagcc atcaacaaag tatatgtgga caaagagaac aacgtgtgga ttgcaaccat
    23281 gaacggcctt aatgtcatta aaagcaagac taaacaagta attttttata cgtccgacaa
    23341 aaaccggcaa aatagcctga gccacaacga tgtgcgatgt ttgtttgaag accggcaaag
    23401 gcgtttatgg ttaggcacag ccaaaggcat tgatattttt accaatgctg cagatgtccc
    23461 tgaaactgcc catttaatcc atatacaaaa taatagcaac aaaaaaagcc tgaacgaagg
    23521 ggctgtacag gttatttttc aggacaataa aggccgcata cttataggca ttgaaaacgg
    23581 cggattggat gtccttcacc ctcaaaacct ggcaaatggc aaatttgaat ttgaccatca
    23641 tgtcaatatc gcagacgatg ataatagcct gagcaacaat tctatttact ccatatttca
    23701 ggataaccaa aacagtattt gggtggggac ccacggagac gggctaaaca tctataaccc
    23761 attgaacgaa aagtttgaac tggtacaaaa ttcaacaaaa acatcacggc ttattatcaa
    23821 taaccaggta aacgcctttg ttgaggatga aaaatacttg tgggtaggaa cagaaggcgg
    23881 actaagttgc tatgacaaaa agacccgtac cagccgtcac ttcgaacaca acccggcaaa
    23941 taaaaaaacg ttgcgggcca atgctgtact tgccttactt aaagacaaat caggccattt
    24001 gtttattggc gggatggaaa tggggctgag ccaatttgat tttgcatcgg ggcatttcat
    24061 tcattataaa gccaacccgt cagataccaa ttcgctcaga agcgacaaca tatttgcttt
    24121 gtaccaggac tctaaaggaa gaatttgggc aggacaaaat tgtgggggcc tttctttgtt
    24181 tgattttaca acgaaaaagt tcaaaacatt tagaaaaaac aaagatgcag atgattgcat
    24241 caaagctatt gccgaaaccc ctgacggtaa attatggctg ataaatgcca cctccattga
    24301 atgtttcgac cccgaaagtg ggagctttac aacatacacc aacaaccctt cagaccctga
    24361 aagcctcagc agcaactatg gtttttctat ttttaccgat agtaaagggg cactgtgggt
    24421 tggcaccgat gcagggttaa acgtttttga ttataaaact aagaaattca aaaagaaata
    24481 taccataaaa aacggcctgc ccgataatgc tattaaaagt atcaatgaag acaattttgg
    24541 gaatctatgg attgggacag ataaagggct ttcaaaattt gtaaatgcaa cagaacaacc
    24601 atcaaacccg aggtttatca actacaaagt tcaggatggc cttcaggcaa acgaattcag
    24661 gaaacgggcc acatacaaaa atgcccaagg caagctttat tttggcggaa taaacgggtt
    24721 caatgcattt tatcccgaca gcattgctga aaacaaggct attcctaatg ttgttattac
    24781 cgaattgctc atttttaacc aaaaagtaaa tgttggcgat gctgattcgg cactaaagga
    24841 agatatttct atagcaaaag agattacttt gtcatacaga cattctgttt ttacgctgaa
    24901 atttgccgcc ctgaacttta tagcccctgc caaaaatcaa tacgcttaca agatggaagg
    24961 atttgacaaa gaatggaaca tagtgggcaa taaaaatgaa gctacttata ctaatctttc
    25021 tccgggcaaa tatacatttg tggttaaggc cagcaacaat gacggtatat ggaaccaaaa
    25081 aggccgctcg ctgaccatca ccatcttgcc cccttggtgg aaaacctggt ggtttcaaac
    25141 cttgttttat cttttcattg gttttatgat atttgccatt atccagttta gaacggcctt
    25201 ctacaaaaaa caaaatgaga aacttgcaca aatggtaaaa gaacggacat tggaactgga
    25261 agaaataaat gttaacctgg aagaaaaaca agaagaaatt aacgagcaga acgaagaatt
    25321 gcttacccaa cgggcacaac tcgaacatac caacgaactg ttagttgaac atcagctgca
    25381 aataagccag caaaatgaag agctgatgag ccaacgcgat gcattggaaa tagccaacaa
    25441 agaacttgaa aaacagaaag ccaaaatact ggaacagaac attgaacttg acaaacaccg
    25501 aaacgaactt gaaagcctgg tcgaaatcag gactcagcaa ctaatccagg ctaaagacaa
    25561 agccgaagaa gctgacaggc taaaatcttc gtttttggcc aacctatcgc atgaaatcag
    25621 gactccgctc aacgccatca ttggtttttc caacttagtt attgatggca tcgatactcc
    25681 ggaagaaaaa gaaaatatcc gacagattat ttatagcagt agcgaaagct tgttaggcct
    25741 aattaatgat atcattgatt tttcaaaaat agaatcagga catattgata ttatcctcaa
    25801 agaagtacca ttgaaaaaaa taatggctga tattcaaagt atttttcaat tacagcttaa
    25861 acgacaacag attggcactt ctcacaacat tgattttagg gtaattgcaa atgagcaact
    25921 aaatgatatt tatctcgaga cagatgaggt tcgcataaaa caaatacttt ctaacctgat
    25981 acacaatgct attaagttta ccctcaaagg ctctattgag gtggggtgtc atgttttagg
    26041 ggacaatgaa attgttgagt tttatgtaaa agatacagga attggcataa aaaaggaaaa
    26101 cctgcaaatc atattcgaac gtttcaggaa aattgaggat gacaaaaccc atctccatcg
    26161 tggtgcaggg attggccttg ctattaccca acagttggtc agtttacttg gcggacaaat
    26221 cagggttgaa tctgaacatg gcattggtac aaccttttac tttactatac cagtcaaaaa
    26281 aaatcctgag ggcaaccaaa cacaatcatc aaatgccatt gccgaaaaca ttaccgattt
    26341 caaaaacgtt aaagtattgg ttgcagaaga tgatttggct aactatgcct atatcgagag
    26401 actgctttta cgtttcaata tcaatgttgt acatgccctc aatggtacgc aggcaattta
    26461 tcttcttacc aaaatacccg atataaagct tgtgctaatg gatattaaaa tgccccttct
    26521 tgatggcatt caggctttgc accagattaa agaaagaaag atagacatac ctgttgtggc
    26581 ccaaacggcc tatgctttgt cgcacgaggt agtaaagctc aaagaagagg gttttgacga
    26641 ttatattgcc aagcccctga gcctggcaga catctcgagg gtactacatc ggtggataaa
    26701 gatttaagat gcatgtaaaa acattattag ccaaattacg gccaatcttg aatttttgta
    26761 accttcttat gccggattgt cgttataaat tattgaatta caatttttta atagactgag
    26821 cattttctat cgcgtaattt ggttttaaac aattagtttt ataaataaaa aggctgtgca
    26881 aattatcttt acacagcctc gtaaaacaat ttttaacaat aatttttaat aaggggttac
    26941 cttaatgtta attgcagcag aatgtaaagt atcgccggca agtaccgaaa taactttcac
    27001 ttttaacaca ccttcttcac ctgctttaag ttttagggca agggcggcag tattaagggc
    27061 tttgtaggtg accaaagccg aatcgttata tgatactgat gaaagcaatt tttgcggttt
    27121 gacaaagttg ccttcaggag tgtcggcaag aatgttatat gcagcagaag cactaaaacc
    27181 gtaatttggt tctttccaaa caaactgtat ggtttgtttg tcattcgcag cagtaaggac
    27241 ataggcagaa tttggggccg gggtaataat gtcgggtgtc ccggggttgc tattaagcac
    27301 aaccctgtct tcatcttttt cacatgccag agtgaataag gttattgcaa gcaaagcgat
    27361 atatttaaaa gtcttcataa ttttaaaatt tagtttgttc atacattaat agccgggatt
    27421 ttgtttgagg ttttggtttg agtttaggtc actggccgga atggggtata aatctaaatg
    27481 ttcatcagta gaaaccccgg cagcgacgtt acctttccag ggccatacat acgttgattt
    27541 tgaaaattgg ccaaagcgga ttaggtctgt acggcgatgc ccttcccaat acaattcgcg
    27601 gctacgttcg tctatgataa attgaaggtc aatggtgcta acattcccgg atgtaccttt
    27661 ataagcccgt tcgcgaactt tattaacata cgtaattgca tcatcaatgg tagctccaga
    27721 acccccgcga aggatagctt cagcatacat caggtaaaca tcagcaagac ggaatacagg
    27781 ccagtcagta ttgacaaagt cgcctctatc ttttggcagt ttacggtctt tgttcatatt
    27841 tcggaatttg gtaacggcat aaccattggt aaagttggca atgtcagtaa tttcaagtgt
    27901 ttggccatcg gtatgaaaca atgccctggt atcaatagtg ccggttacat cagggaattt
    27961 gcttacaaaa gcgctggtaa cgcgattgcc accccatccg ctggcaatac caaagctatc
    28021 ggctttcatt gagcccccta cctgggcatg aataatataa ttcatgcccc catatccggt
    28081 actatgaaaa aagtcatagg taaaagcaaa tattatctcg ggactggtat tattgtcagc
    28141 cataaaattg tctgaataag taggggccaa agaatatcct gcatcaataa cctttttgca
    28201 ataagtgata cattcggtat ttctttcagt gcctgtataa actttggcat tgaggtaaag
    28261 ttttgccaaa agggtccatg cagcagcttt gtcggccctg ccatattcga aacggggttc
    28321 gcccaaatca ttttgggctt cgagcagttc ggtttctatg tatttgaaca agtcggcccg
    28381 gttaacctgt ttgggattaa agaacccagg tttgtcagcc tcagtaacaa aaggtacatt
    28441 cccgaacaaa tcaatggcat gccaatagct tagagccctg ataaaccttg cttcggcttt
    28501 cataaggccc attccctgca cagagctgtt gtcgttggca gtcctaataa attcgttaca
    28561 taacgagatg gtgtaataca accgggaaaa catggtagta ataaactcgt tgttgggggt
    28621 ccaggtttgc caatgaaagt cttttattgt gccatctgcc caacaattga cagcttcgtc
    28681 agtactaagt tcctggcaat tccaataaag cctcagataa ttggaaaaac cttcatcaag
    28741 gcctgcaata tcaggttgtc cggcagggcc ttgttggccg ctaagcgaaa tactggtata
    28801 aagtttagcc aaagcttgtt tgtaatcatc aggcgttttg aaaacattat tggctgtagc
    28861 aagttttgaa tcaatggggg tcacatccag atccttagta catgaggata tcaatataag
    28921 ggatgcaaca atggggaaaa ctatattttt tttcataaca ggcatattta aaattttgtc
    28981 aaaaagcagc aattaaaaat tcaatgaaag agagactaaa taagtccttg gccttgggta
    29041 gatgttttta tcaatgccgc tttctacttc agggtcaagg ccggagtatt tagttatggt
    29101 caagacattt tggacagcaa aacctaacct gccactaagt ttgttggtga tgattttatc
    29161 aaaattatac cctacgctga tattgtctaa tttgaaaaat gaagcatttt ctacgtagta
    29221 atcagaccaa tattgagggc ttgcaaaatt agtttttgac acggctttag gcaagttgtt
    29281 gaagtatcct gattgtagat ataattgtgc atagttggcc ttttcggaca aaacattgtt
    29341 gtaaacatag tttccaaggt tgatacggct cgaaatagag aaatctacct ttttgatgtt
    29401 tcctcttgtt gaaataccca tagttacgtc gggtgccggt tttttgtaat aatacttgtt
    29461 gaggttattt ccacttacat tgcctttttt gccactacgg tcaacataaa gcccttcaac
    29521 aggccttccc tggctgtcat atacttgttg gtaaacaaag aatgaattgg taggttgccc
    29581 aaccgcacga atttggattt tgtttcctac tccgcctgaa atatcgccat aatccatacc
    29641 cctaaaagta gggtcttcaa tatttctgag ctttgttatt tcgttttcgt tgtaagctat
    29701 gttatagcca atttcccaaa aataatcttt ttgcgatacg attactccat tgaggctaaa
    29761 ttctatccct ttgttttcca ggttaccaat gtttgtagtt aaataattag aaaagtttgt
    29821 cccggcagct attgggatat tactaatcaa gtcattagtt tttctgtaat acacttctaa
    29881 agcaccgttt agcctgtttt tgaagaaacc ataatcaagg gctatgttag tagtacttgt
    29941 ttcttcccat tttatacggg catcgtaagg attgggcctc gatgtgttaa taaatgtgtt
    30001 gccaaactga gtttgtgcag tagaagaact gctttggtat gtggccaaat atgggtaata
    30061 ggtatatgat ttttccgggt ccccaatatt ttcctgacct gtaatcccat aacctaaacg
    30121 aagtttaagg gtacttaacc acgaaacgtc ttttaaaaag gcctcatcgt taattttcca
    30181 ccccaaagca acagaaggga agagccccca acggctatct ttgccaaatt ttgacgagcc
    30241 atcattacgc agggtaaaag ttattaaata cctgtctaat aatgtataat tgatacggcc
    30301 aaagaatgaa accaggtagt tttcactttt atatccggta gtgtcaattt ttataggggc
    30361 taccaggttt tgttttatat cttctttttc tctgtaaaaa tattgccagg aatagccggc
    30421 agtgatgtca attttactgt tgatagccga taaatctttt acataattca ggtaaaaatc
    30481 taaaagctgg ttttttaaag tttgggtata agtttgtttt cttccaattc catcacggaa
    30541 agtaaaggca gcaatggtat cgcagttgtt gtgcccttta cttgagctca aatcatagcc
    30601 aacattgagg ttagccctta gctcgggcaa aaagtgaaat ttataatcaa attgggcatt
    30661 cccaatactt cttttaacag tagctttgtt atcagtttgc tcaatcatgg ctacagggtt
    30721 ttttgtagct atggcattag ggtctccatt aacattgatg ctcccgtcag ggtttacttc
    30781 cgaaatattg acaaaggtag tatatccccc aaacctggta ttgccattca tgacaggttg
    30841 cgtagggtca aatgctacag cagcaccaat agcatcgtcc acactaaagt tattgtcagt
    30901 gtacatgcct tttacattaa ggttaacttt tagatggtca tcgaaaaatt tagggtcaat
    30961 agccaatgcc agggttttcc tgtccatatt ttttttcttc aaaataccat cctggtctgt
    31021 ataacctaat gaaacacgga aaggcatttt tttaattccg ccggatatac tcaggttgtg
    31081 gtcttggttg aaagcatttt gatatatctc cttttgccag ttagtattgg cagtgcctaa
    31141 acgttgaagt gaagctgctg ttacgccaga gccttttata gtggaccttt ctaaggccaa
    31201 ttgacgcaat tcatcccctg tcatcacgtc ataatagtca atgaccgtac taacagaggt
    31261 agtattgttg taactgacta taatattccc ttctttgcct tttttagtag tgataataat
    31321 taccccgtta gaagctcttg aaccataaat ggctgtagca gaagcatctt ttaatacaga
    31381 aaaagactca atatcactgg ggttaatggt agaaaggctg ttaggctgtc ctgaaaccgg
    31441 ggtgttatca atagggacac catcaataac aataagcggg tcattactgg cggttaatga
    31501 agaaccgccc ctgacacgga tagtagcccc acctcctggt gaaccatcat tggtagtgat
    31561 tacaactccg gcagttttgc cgataataag gtcctgagga gtagtaactg ctcctttatt
    31621 gaactcttta gtgctaacaa cagctacaga gcccgttgca tcagactttt ttacggtacc
    31681 ataacctata accacgattt catcaagctt ggttacagac tcttttagcg aaatatcaag
    31741 agtagtgctt gtttgtgcaa caacctcctg gttttcgtac ccaatgaatg aaaaaacaat
    31801 aacatcattt ggattagcgt tgatagaata aaccccatca aaagaagaaa gtgtaccctt
    31861 tgttgtcccc ttgattgaga tatttacgcc cgggataggg ctaccgtctt tagcatcggt
    31921 aacagtccct ttaatggtca attgctgggc aaagcttgta agcccaataa ttgttgccat
    31981 aatcgaaagt aagatttttt tcatatgact actttttttg tgtttagttc gtttactgca
    32041 aaagtaaaat atgagttaag cgttaaaaag atttaagtta ataaaatttt acgaaaaatt
    32101 aaaaaacaag gcattttaag cttgaaaagg caagttgcaa acgtttgcgg gaaaattgca
    32161 ggagagaaca aaagaaaaaa aggcataagt gctttaaact tatgcctttt tctttttctt
    32221 cagttattgc aagcgaatca gttgttttgc cagtgtgcta tggctttata attatcattc
    32281 aatgtagctt tacattttaa atggcttata acaagctctc tcttgtcgtg gtcagataaa
    32341 gacctgaacc atgcagcttt atccggtgtt gacttttttt gtacccactg aaaaaagcag
    32401 ttacatccat gtttgccacg ctgacaataa aacatggaat tgaggatgag ttcgtcaaaa
    32461 tatatggttg agtttacaat tttcataagt ttatcaagtt ttgttatatc aaaattaata
    32521 catcaaaaca gctaagtaaa gagctattat actcaatttt agatagaata aaccgaaaag
    32581 tgcgatggta gaaatacgat gaaatgtttt tcattgctaa aaaaagtata tttttggtat
    32641 ttaaatactt atttatggca aagattgtaa acctgtcaga agcatcgtca atagcattac
    32701 acgcaatgat attaattgga agggccaaag gaggctcaat taatgttgat caaattgcag
    32761 aagtaatcaa ctcgtcaagg caccatgtgg caaaggtaat gcaaaggctt gtaaaggaag
    32821 gctttgtcaa ttcatatcgt ggccctcatg gcggtttttt tctggttcga aaaccagagc
    32881 aaataaattt gcttgaaata tatgaagcta ttgaaggaaa ggtaagccca agtgcatgcc
    32941 ctatggataa accagtatgc aactttggca tgtgttttat ggataacatt gcccgcgaac
    33001 ttactgtaca gtttaccgat taccttaaaa gccaaacttt agctaaatat ttgtaagctt
    33061 atcaactaat aagcgcaggt tttaaaaggg cctacacttt gcattgatga atattcttgt
    33121 tttttgtaaa gtaaaaaata aggctttcat ttttttcata actcagtttt ttttactaac
    33181 ttcaattaaa aatgagcttc taagaatttt tttaagtggt tattatcaag tttacttctc
    33241 tataccaata ataatattta ttatgaggac caatgccact aaaaattttg ttgaccagac
    33301 aatagatgca ttgtcaaaca atagtgacac gctacccgaa aaagaaaaag atgtttatac
    33361 tttacttgaa aatgtatctg ttgcaaaaga tatattagaa agtgaactga acaaaaaaga
    33421 aaacgaaata aagcccaata aggggaaaaa ttgtatccag tcttttgtaa atacattgtg
    33481 cggatatata ggggcatacc aggtatttat cgggcgcatg tctgagagta tagaaggcat
    33541 tgttgaaatt attttgagca atgacattga aaatgctgat aagattaaac atgtaaaaat
    33601 tgaagggact ccatttagta aatgcaaaga aatagatatg ctggtgtgta cagaagcacc
    33661 gttaaaatct tttccgggcc ttacaaaaat taccagtatt gaacccaaaa gcctgataca
    33721 atatgttcac aaaaagtcag gggctgtttg tcttgccctc ttcaatagtt atgcaatgga
    33781 cgtatccaag actgaagaag ttctggagtc ctttatccct gaaatggtta attttgtaaa
    33841 cgaaaatgaa aatatcaaca atatagaaca aagccttgcc aggtataagc atattgtaga
    33901 aacactttca gaatatattg taataaataa aattgaaaat ggcaaggtca taaacatctc
    33961 gcatagtgat atttgtcaaa aggttacagg gtattctatg gaagattata agaatgaccc
    34021 tgatttgtgg ctcaaaatgg tgtacgaagc cgacaaaagg tacgtcacag agcagctaaa
    34081 caaaattata tttgggatag aggtaaaagc ttttgaacac aggatatacc acaaaaacgg
    34141 cacagtaagg tggattcgca atacaccggt acttttttac aatgccatgc accaaccagt
    34201 tgaatatgtt gccattatca atgatatttc agagctcaag caagtagaac tgaagatgat
    34261 agaaagtgaa aagcactata aaatgctttt caatgagatg aacagtggtt ttgggcttca
    34321 cgaaatgata tatgatgaaa atggcaatcc ggcagattat cggtttatag aggtaaacct
    34381 tgcttatgaa aggcttactg gtttaaaaag agaggctatt attggcaaaa ggcttttgga
    34441 aattcttccc ggaattgaca aagaatggat tatgcgctat gggcaagttg tcaaaacggg
    34501 tatccctgaa acatttgtca aatatgagcc attgtttgac aggcattatg aagtggtggc
    34561 atattcgccc gataaagatt tgtttgcagt agtatttaat gatgtcagcg atagggtaaa
    34621 agcccgcgaa cagttagaag agcgagaaaa ccgctatcgc agcattgtgg agttgtcgcc
    34681 tgatgccatt gtaatttttg ccaacgaaga aattgtatat gccaacaaca aagctgccaa
    34741 tctatttggc ttttgttcag ctcagcaaat gcaaggcaga aatgtgaaag aatttttcag
    34801 aaatgcctat attgatgagg tcaaattaaa tgtagtcaaa gaatttgaag aaaaactgcc
    34861 cggacggtta gttgaagagc aaattaaaaa gcaagacggg actttagttg aagtagaaat
    34921 ttcggctgcc cctgtttttt tggggaaaaa aaccatacaa ctaataatca gggacatcag
    34981 ccagcgtaaa cgaaccgaag ctgcccttat acgcagtgaa gaaaggtttt tgatggctat
    35041 ggatgctgta aatgacggtg tttttgagtg ggacctcaaa gcaaatgagg tatattacag
    35101 tccgcgaaat tataacatgc tcggatatga agtgaacgaa ttcgagccaa aacatgctgt
    35161 atgggagagc atgatacatg aagaagataa agccaataac cttgcagagt ttcagcgcca
    35221 tttgcggggc gaaagtgaga tgtatgagct cgaatacagg gttaggacaa aatcgggcca
    35281 gtacaaatgg atttctgaaa ggggcaaaat tttagaaagg gacaaaacgg gcaaccctat
    35341 acgtattatt ggaatccata gtgatattga tgcccgcaag gcgatggaag aaaacctcag
    35401 gaacgaggca gagtttatca acaacctgat gaacattgcc ccggtgggca tttttatagt
    35461 gacagttgac ggcaaaatac catttgccaa tgacctggca atacaaatga taaggttgaa
    35521 gcctggccag gacggatact ataatgttcc acaatacaag gtttacgaca gcgatggcaa
    35581 agaagttaat tttcaggatt tgccaattta taacgctttc aaaagtatga cctctgcatt
    35641 acatcaaagg ttttggtacg agtctgaagg gaagaagatt tttgtagaaa ccaatatgcg
    35701 gccttctgtt tcgctgtacc aaaaaaaaga ggggttcatt atagttgtgt ctgatattac
    35761 caaccagata gatgccgaga ataaattgcg acaggccaaa gaaaaggccg aagagagcga
    35821 caggctaaag tcggcctttt tggccaacat gagccacgaa atacgtaccc cgatgaatgg
    35881 aatcattggt ttttccacct tattggcaaa gccagatttg acgcaggaca aacgcgataa
    35941 ttacttgaat atagtccaaa acagtgccaa tcagctcctt tctattatca acgacatact
    36001 ggatatatca aaaattgaag ctgggcagat gcagattgac cattcgcacc tcaagctcaa
    36061 tgatttgctt gacagtcttc accttcagtt tataaaagaa aaaaaagaca aagggaaaga
    36121 acacgtgggt ttgtttttaa agaaagggct tttggatgag gcttcgcata ttatcagcga
    36181 caaaatacgt attggacaaa tactatctaa cctgctgggc aatgccatca agtttaccga
    36241 aaaaggcagc attgaatttg gctacaccat aaataataac aaatggattg agttttacat
    36301 ccgcgatacg ggtattggca tcccggccga aaaaggcaag ttgatatttg agcgtttcag
    36361 gcaaatagat gaaactacag ccagaactta tggaggtacg ggtttggggc tttctatcag
    36421 taaaggcatt gttgatttga tgaaggggaa aatctggttt gaatatgaaa taggcgttgg
    36481 tacagtcttt tattttacag tgccttacaa tccgctgaaa aatgtacagc aaacagaaac
    36541 tgttaaatta gtatcagcag acgaaaatca tagccttgag ggatacgaag tgttagtagt
    36601 tgaagatgtg ccggagaact tttacctgct gcgcgaaata cttgaagaag ccggggcgca
    36661 catattgtat gcttcagatg gaaaaaccgg gctttcaata ttgaaaaata ctgaaaaaat
    36721 agatttcata ttgctcgata tcaggcttcc ggatatcagt ggatatgatt tggcccgaca
    36781 gatgagaaaa gaaaagcccg atgtcccaat tattgcacaa acggcttacg ggctttcagg
    36841 cgaccgcgaa aaagccctgg cagcagggtg cgtagattat atttcaaagc ctattgtggc
    36901 agacgaattg atgaaagtga taaaaagaaa tataacgccg aagcaataga gaaatcagga
    36961 cttcaaagta aattgagggg gtataaagaa atactaaccg caaagtacac gaataaaata
    37021 aagtacgcaa tttttatgtg ctgactatta acaccttgtg ttttttgcca agcactttgt
    37081 ggcctctgca gttagcttat ttcgaacaca acctttaaaa tttttattca atcgacagaa
    37141 ttttaaattc ggtgcgacgg ttgacctgat aatcttcttc actacacttt atgccatttt
    37201 tacatttgtt taaaggaatt gattcgccat aacctttacc aacaatacgt tctggggcaa
    37261 tacctttaga aatcaggtag gcaactgctg cttttgcgcg cttatcagaa agggctaaat
    37321 ttgcggcatc tgagccacgg gaatctgtat gcgacctaag ttcaatgcgc atcttaggat
    37381 aattgttcat catatctaat agtttgtcaa gctctttagc ggcatcggga cggatatccc
    37441 atttcccaag gtcgtaatat atgttttcaa cgcggataac atcacccaca caaagaaccg
    37501 ggaacatttc agtaaaaaca ttatcgtctt caagctcttt tgtgctaata tttacagcat
    37561 tggcgctaca ctttttgcct ttgacagata tttgtactgc aatattataa ttggcttcag
    37621 gttgaaggtc tgcaatgaaa ttaccagaag catctgttac aagttcggtt ttagagctat
    37681 cagcgagatt agtaacaatg gctttaatat tagcaaaagg ctgtttatcg tcagcagcat
    37741 agacctttcc gttaataaca aatttgggga tgcgtttttc gaggggaata cgaatggtgt
    37801 ccaaattagt taattcgctt gccgtaaaat ttaccttagt ttcaatatac ttatcgcggg
    37861 ttgcagtaaa ttgataatct tttgatggtt ttacgacaag aggggctaag cccaggatat
    37921 ttgtggtttt attttcgggg tcagagtttt cattagagat aataaggttt acagaagaaa
    37981 gtggttgcaa attttttttg tcataagtca aaatgaccag gttacgggaa atttttttcc
    38041 aacggtagat atcatcattc ccgacatcat tgaagcggtc tgtactaaaa tagccttcat
    38101 tgccggaggg agaaataaca aacccaaaat catcactttg ggcattaata ggaaacccaa
    38161 gatttacagg attttcaaaa ccatcattta tggttttagc cataaaaata tctaagcctc
    38221 caagcccggc atggccgtta ctggcaaaaa acaggttgcc ttcattatct ataaaaggga
    38281 acatttcatt ttcggcagta ttaatgacag aacccaagtt ttgtggttta ctccaatgaa
    38341 aataaccatt ttcgttttta acccgatgag ttttgtagat atcagtacct cctatactgc
    38401 caggcatgtc agaaacaaag aaaagagtgt tgccatcggc cgataaggct ggatggccac
    38461 aagagtattc gttgttgtta aactccaatg ggacaacatt tacccacttt tttttagtac
    38521 tatcccaatt ggcttggtaa agcttgagtt tattaatttt ttctttactc ttgccaaaaa
    38581 gaagattatg aaaattgctg cgcgtgaaaa taatggtgtc gaaagtttta ttaaaagcta
    38641 cagggccttc atggtaaaga gagtttagcc caggcataaa ctctgtggat aaagatgaat
    38701 caacaagggc aaaatataaa tcgaggtatg agctgttagt ccaagagtac caaagcctga
    38761 taataccttt tttttcgcgt gcactagcat aaactaagcc atttttgtaa tatgcaggag
    38821 aaaagtcaga aaaactgctg ttaaatggca tgtttttgag caaatacgat gcagagtctt
    38881 tgtaaaatat tgaaacatta ttataagcgc tggagaaagc ttcaccctgg gcatctgcag
    38941 gttttagtat tgcatatttt tcataccatt ctttcgattg gttatacttg ccatttctgg
    39001 caagcacctg agccagcctt aaaacagaga ttgtatcatt tgggaaagag tcagtaatca
    39061 attttaacca atattcggca tgttggctat cgttgatcct atagtatgcg ttggccagtt
    39121 tatagacaat ttccatgtta taaggttgtg catcgtgcaa ctcgtggtac atggggattg
    39181 ccttgtaata ctcaaaacgg ttattgtaac ggtcggccac tttctttttt atagtaaaag
    39241 actgggccga agttgtcatt gcaaaaaaca ggacgacgaa aaaaagaagc cccacccgac
    39301 actttttaag atttgacgtt cccataggca caaaattatt ttctaaagtc cgcatagctt
    39361 gttcggtaaa agcagaagaa ctcgttgata aaaaacggga ttcataggtg tttgagttta
    39421 gttctaaaca tgcttgtttc atccgtataa aattttaatt tgttgaaatt taatcattta
    39481 agccatcatt atttgccatt taatgtgagc ggatggaaca ccctcattat tcatgtttgt
    39541 ttttgtttgg cttcaatcat ggaaggttga tcatatttat tggaatgata gcctgctccc
    39601 acaataaata ccatcgtgtt tgcgatttgc actatcatgg gtgtttcatt ttatgattat
    39661 cattttttgt ttccataatt ttatttattt cgtccccttt ttgagaagcc agttgtaaca
    39721 atatattcta aaagtacctt ggggtaatta ccctggggtg agcaaagtca aaatcatatc
    39781 tgagcataat ttcatgggtt ccggaattgt agttgcgcaa gtttgtaagc gaatattcaa
    39841 aggcataacc gaagtacaac tgtggcaata ctttaatttc ggtaaatgcg ttaacagcat
    39901 caaagctttt gtatgaaacg ccaagcccaa gagtttcaaa taaccaagcg gttgtattga
    39961 tatccacctg aggatttgcg ccataaacat aaaccattaa ggctgagggt ttaattttta
    40021 ggtcactatt aatatcaaat acataccctc cggtaataaa catgtggtta ctttgcgaaa
    40081 agttgtaagc atcaggtttt gaaaagtatt tttcgagcaa cgagctgttg ataaaagaag
    40141 gcatacttgc tccaatgtaa tatttatcgg taaaataata tgcaccaaaa cctatattgg
    40201 ggtacgtatt acttgtgttc tgcgaaaagg cattgtcggt tgacctgtta cggttgtcat
    40261 aattgacatc ggtgtagttt gagctaaaaa agctcagccc catttgaacc ccaagtgcta
    40321 aagtagcttt tttcattggt attttgtaag aataaatacc atttaaagcg ttgcgtttag
    40381 tgatgccaat tttatcagct atgactgtca taccaagtcc aactttattt tttcgaaatg
    40441 gagcatctgc cgagaaagtg aaagtcttag gcgctccagg catgtttacc cattggtttc
    40501 ggtacagcaa ggtagcgctc aacacctcat gggtacctgc ataggcagga ttaataacca
    40561 aaccattgaa ttgatattgg gcatacaaag ggtcttgttg agcatttaca tttgcagcaa
    40621 atacgattat cgatataacc gagattttaa aatattttgt catggtcgat atttttactt
    40681 gttaatagtg atataacctt ggattggctt acggtttttg ttaaatttag caatatagaa
    40741 ataagttcca tcgggcaaag ttgaattaac agtgattcct atatttgttt taccgtccca
    40801 gtcatttttg taatttttat tttcgtaaac aaggttgccc catcggttat atacttgaag
    40861 atgaataata tagcgttgtc ctccacgaat gataaagtta tcatagtagc cgtcaccatt
    40921 tggtgaaaat ccttcaggga caaaaaactc aacaggttta atgttgacag gggtgggttc
    40981 tccatttgct atttcaggaa taatgccatt tatagaacta gctataacgg ttttgccatt
    41041 tatgctttta gctgaggctc ttgcaatatt gttataagtg atagtggtag tttcatcacc
    41101 ttctacataa acattgaccg taaatgtaat ggttgccatt tcgttgcctt ctaaagtact
    41161 tccttcaagt aacagcattg tatcaatggc cccatcatag ttgggattaa caatcaggtt
    41221 gccatttgca gaaattgaat tgtgttttac cttaaaaatg gcaggttcgg gaaatacact
    41281 tatcaggttg tcaccaacaa gtacatcgtt gagttttgta cttcccatat tttttacata
    41341 aatggtataa gtaatatcat agctgtcatc agcctgaaca tcaggagttg caacactttt
    41401 tgcaatggca attgcaggga ccggcaaaac gtggataata atagtgtctg aggtgcattg
    41461 tgaagggatg ccgttatcgc agatgttgag tataatggta tcttgtcctg taaacccaat
    41521 gtttggggta tagattaggg tgccattata aatttttact gtacctcctg ttggatttac
    41581 cagaacagaa acgatcaggt cagaagaaga attgtcaata tctgtaatat ttgatagcag
    41641 gtcgatattt agagctgtat tttcattggt tacaccatta tttacagaac atacaggttt
    41701 atcattaaca ggttttacag taaatacaat ccatgcattg ttgcataacg aaggagagcc
    41761 attgttgcat attaaatact gtactgagtc ttttccgaaa taatcttttt cgggggtata
    41821 aataattgtg ccatcaaaat tcaacataat agcaccatgc acaggattaa tgctaactaa
    41881 agcagtaagg ctcccgccat tggggtcaaa atcattagcc aggacattgc cgctaaaagt
    41941 attatcttca ttgatttcaa aaatatcatt aacggctacc ggagctttat tgctgtttat
    42001 tacagatata acaatcattc cgctactgca aagcttaggg gccccattat cgcatacact
    42061 gaaatacaga gtatcattgc cagcaaaacc agttgatgga gtgtacacta tatcattacc
    42121 tttaaatttg atactaccat ttgaagggcc attaaccaca gatgttgtca aaatatcact
    42181 atcggggtca aacgaaatgg ctgttatgtc aataataacc gcctcatttt cattggtatt
    42241 tttcagaata gtatcattgt caagaacagg agcatcattt acattggcaa cagtaatata
    42301 tattgtatca gttacacatt ggacaggtgt tgtaatatca caggctgtga aaacaatggt
    42361 gtcattgcca aagtaatttt tggcaggaac ataaaccaca tctccattgg cattaataat
    42421 agcattacca tgctttgggg catcagatat gctaaacaca agggaatctc catcaggatc
    42481 agacactgag ttgcttacta caataacaac ctgaggaata tcttctgtag tgctaactac
    42541 aaaggtagta ccaggatcaa ttatcggagc attatcagaa ggcgcagaat ttattttgat
    42601 aacaatatat ccggttacac acttgttagg tgtaccattg tcacaagctg tgtagcaaat
    42661 agtatcattg cccgaatatc cggcaatagg attgtaagtg atgttgcaac cacttacaga
    42721 agctgagcca tgcaatgcag cacatccaat gataatagtt ggtgtagcac cttctgcatc
    42781 agttacattg gaacatacat caaaggtagt gtctttgttg attgggatag acattttaat
    42841 ggtgtctttt gtcaataccg gggcatcgtt tacagcagta acactgacag atacacttac
    42901 tgtattgcca ttcagcaaac catcattggc tttgtatgta aaggtatcgc tggttgtttc
    42961 agtaccgtca tgagtataac taaacgaacc gtctgcgttg aacgccaggc tgccatgagt
    43021 tgtaccactg accaatacag caaacaatgc atcgccatcg acatcgttgt cattggcaag
    43081 aacgccgggg gcactgatgt tcaggctgcc cccttcagct acggtatagc tgtcgactgt
    43141 tgataccggt gcattgtcat tgacatttgt aatatttatc gtgatagtag catcatcact
    43201 ggttacacca tctgaaacat ggacggtaag ggtatatgaa ctcttggttt cataatctaa
    43261 agcgccattg accgttattt caccagttga agcattaata gcaaaggctg tgcctgtatt
    43321 tcctgaggta atgctgtaag ttagcgcatc ggcatcagca tcagtggctg atactgttcc
    43381 aacggaagtg gcagtagctg tattttctgc tataaagaaa gtggtatcat tgacaacagg
    43441 agcgtcattg atggcagcta tgctaatagt tattgtttta tttgcacttg cataaagggc
    43501 cccatctgtt gcgttccagt cgaaggttgt agaaccgttc caatttgcag aaggaacaaa
    43561 agtaatactg ccaaggtcag cggttccaat tacatcgttt atagctatgt ttaaacctga
    43621 aagcttgagc aatccatttg caggaaggct gattaccttg atactactaa gagctgtacc
    43681 ttctacatca gcgtaattgc cagtaaaatc agtaacagca aaagagaaaa tattgtcttc
    43741 tgttccgttt acggtaaagt cagaaacaac aggagcgtca ttaaccgctg taacatcaat
    43801 ggtaatggta taatcagatg ttgaataaac attcccgtca ttaactttga aactgaatga
    43861 agcataagaa attccatttc cattggatgc cggactgtat aacaattttc cggcatcgat
    43921 atcagctttg ctgattgtcc cattgagtgc aattggctct cctgcatcaa gtatattatt
    43981 tgaattggca tctataaata aaactccaat tgctggtaac atggttattt gcaaatgatt
    44041 taatacatcg ccgtcaacat cagcataacc caaatcggca gttacaatgc taagataagt
    44101 atcttcattc gtagaaaggg ctttattaac agccgtcggc gcggtattgc ttgaattaat
    44161 atttatagta attgtatagg atgatacaga ataatctatt ccgtcataga ctttaaaatc
    44221 aaaactggca taaggagctc caaaatcatt gaatggagct ttgaatttaa actgtccggc
    44281 atcaatatct gctttattaa tagttccatt cattgctatt gcttcgcctg catcaatact
    44341 tccattgaga ttggcatcat tatacaaagt ccctgaagcc ggaaggttag tgattttaac
    44401 cagcgttaat gcatctcctt caatatctga atatccaaaa gaagcaacat tgaaaacttt
    44461 ttgaatattt ttaattgtag aaacagtaga attagatgcc gtcggcgcat cgttcacggc
    44521 tgtaatgttg atggtacggg tttgggtgtt gctaccagca gtgccgtcat tgacagtaaa
    44581 agtaacggtg cgtgccgcgg tagttgggtt gtcactgctg ttggcgtatt ttactgctcg
    44641 caaagcagcc tggtagttgg ccttagtatc gctgcctgtt aaagtcattg taccagttga
    44701 gctgttccat gtcccggtga tagatgcagt attggtaaaa gacaatacat cttctgcact
    44761 ctggtagccc ccagttatct gtacggtagc cgaggccatg ttggcattgt cggcatcaga
    44821 taccgtgagg gtagatgtta ttgccgtagc aacagcattt tcggtataag tcaaagcccc
    44881 tgcctcaatg gctgccagta ccggcgcatc gttcacggct gtaacgttga tggtcacagt
    44941 tttgtttgct aaagcaaaca aagaaccgtc agaagcgttc caatcaaagg aggttgaacc
    45001 gttccagtct gttgttggca caaagctaat attaccaagg tttgcaaaag caatgacatc
    45061 atttatcgca atattcaccc ctgaaagttt aagaattcca ttggctggaa gagtgataac
    45121 cttgatactg ctgagtgcag aaccttctac atcagtgtaa ttactggtaa aatcagtagc
    45181 agcaaagata aaggtattgt cttcagtacc gtttacggtg aagtctgcga ctgttggggc
    45241 ttcgttgaca ttagttacgg tcacactaat tgattgcgta ttggtaaggc ctcctccgtc
    45301 ggtaacacgt acatctacaa cataaatatt gtctgtatta gcatctgtcg ggttttcata
    45361 gtttcttcct gaaataaatg atagatctcc agtcaatgcg ttgatactaa ataatgcctg
    45421 atctgctcca cccgatattg agaatgtttt agggtcacct gcatctggat cataggcagt
    45481 aacggcgatt acagcagttg tattttctgc aaccgataca gtagaagaac tggtaatagt
    45541 tggtgcgtca ttaacagcag taacattgat ggtcatatta taagaagaag ccgaatagct
    45601 gacaccatca taaacatcaa actgaaataa cacataagga gaaccatttg catcgggcac
    45661 aggcctgaat ttcaaatatc cagcatctag ttgagctttt gtaacttcat catttactgc
    45721 tagtatttca cctgcctcaa caacattatt gaggtttgca tcattgaata aagtacctgc
    45781 tccgggtaat gaggcaatcc gaattttagt caatacatcg ccggcatcaa tatctgaaaa
    45841 tggaaattct gcagatgcaa aggtcttttg gttatcttca acaatggtaa cagcgttatc
    45901 agcagatgtt ggggcatcgt ttactgctaa aacattgatg gtcattgtat tttctgcgac
    45961 agcatataca cttccgtcat taactttaaa accaaaagtt gcataggcag ttccattagc
    46021 attagcagca ggtttaaact tcaggaaaga gagttgtgtt accagtattt cctgatttag
    46081 cgtaatatct tcgccggcat ttattaccga attgccattt aagtcaaggt acaacgaacc
    46141 ggcagattca agactggtaa ccatcacttt tgccaaagca tctccttcaa tatctgtata
    46201 agggaaatca gtatctgaga atattttatc aacatcttca tttgtagtta ctgtattatt
    46261 ggctgatgta ggggcaccgt taattgaatt gatttgaatt gtccttgaaa ctgtatttga
    46321 attcagtagt ccatcagttg ctgagaagct gacacttctg ttcgagatat ttgggttttc
    46381 agaagtattg acatacttaa cgctctttaa agctgcaagg taatttgctt tagtatcagt
    46441 tcctgaaaga gtcattgttc ctgttgcaac gttccaactt ccggtaatac taccggtatt
    46501 gataaatgaa agcacatcct gcccattctg gtaattagat gtaatctgaa cagttgcacc
    46561 ttgaatatct gtattatcag gatctgccag ggtcagattt gaagtaatat tggtagcagc
    46621 gtcattttct gtgtacaata aagatgcact ttcaatgttt gcaagcacag gggcatcatt
    46681 aaccgctaca atactaatat ttcgacttat agtatttgag tttacagttc catcattaac
    46741 gttaaaagat attgtcctgg ttaacgttga aggatcatct gaagtatttt ggtaataaac
    46801 cgttctgagt gcagaaataa aatatgaaat atcagcaggg ccggtcaaag tgagggttcc
    46861 ggtagcctga tcccatgaag agctaatgaa aatattatct gaaaacgaca gaaagtcctg
    46921 tccatactgg taattattgg taattttaac agtagcgctt tgagcttgag tattatcaat
    46981 atcagtgagg ttaatagtag aacttatggc cattggtccg gcattctcgg tataattaag
    47041 agttgtaccc tcaatagccg aaagcactgc agcctcttgt tgcggagtga cattgatagt
    47101 catggtggca ttgccagaat atgccgaaac atcgcctaca ttgaactgaa aagttgtata
    47161 aggggtacca ttaccattag ctacaggttt aaattttaac ttgccggcat tgatatctgt
    47221 tgctgaaact atattggtgg cagccagtgt ttctccacca tctactattc cattgaggtt
    47281 tgcatcatta tataatacgc ctgttccagg gattgaaaca atacggatac ggaccaaaac
    47341 atctcctgca tcaacatcca tatatggaaa atctgatata gcaaatactt tatccacatc
    47401 ttccaatgtt gtaacagaaa aattggctgt tgtaggagcg tcatttattg cagtaacagt
    47461 aatatttgca tttacagcgg ttgtcgaata ttttggtata gcattgttat cttgcgccat
    47521 ccaggtaaat gaagttgacc cgttccagtt gctggttggt ttaaatttta gcaatgctaa
    47581 ttgtgccgaa gtatatactt gtccaacagt tgtagcttcg ccggcatcaa caagcccatt
    47641 gctgttagca tctctgtata aagtcccatt tgcaggtaat gtagtaatct gaacattggc
    47701 catagcattt gattcggcat ctgtataagt tggggcattg ccaaaatcag aagctgcaaa
    47761 cgccagatct gtatcttcat ttcctgattt agaaaatgcc tggatgacag gaggtgtatt
    47821 gttgcttata gcaatattta cagcagcagc ggtagctgcg taagcagtac cgtcataacc
    47881 attccaatta aatgtagttg tgccactcca tgccgctgtc ggcttgaact tcaaaaatgg
    47941 gatgttggca tttgtgatta ccgcattagc ggcaacagcc tcaccggcat caacaacgcc
    48001 atttgagtta gcatcacggt aaagcgtacc attagcaggc aaagcagccc caatctgtat
    48061 ttgtgtcatg gcatcaccgt ctgcatctgt aaagtgcgaa gtaaaatctg ccagggtgaa
    48121 ggttacatct gtacttacgt tagccgactt attaagggca gcaacaacgg gaaggtcatt
    48181 tactttggta acattaatat ttcttgtttg tacatttgaa ttatattctc catcattgac
    48241 agtataagaa actgttctaa caagggtcga agggttttca ctggtattgg catatttcac
    48301 acttcgcaat attgtttgat aattggcttt tgtatctgtg ccactaaaag atatttttcc
    48361 tgtcgcagca ttccagatgc caacaataga acctgttgca gtgaatgaaa gtacatcctg
    48421 cccactttga tagttacctg taatctgaac ctcagcgcct tgcaggttag ggccatcatc
    48481 gcttactgtc atggatgcag ttacatttgt tgcagcggca ttttcagtat atgctaatgc
    48541 cgcgccttca atagcagcaa ggaccggagg gttgttggct accgagatat taaccgatct
    48601 gaattgtatg ttagaattca ggttaccatc atttactgta aatactatgg tccgggcatt
    48661 agtattaggg gttgaactgt tattccgata agtaacagag cgtaacgcag cttcgtagtt
    48721 tgcttttgta tctgtacctg tcagggtgag tatccctgtt gcaggagtcc agactcctgt
    48781 tattgttgcc gtattggtaa attgtaatac atcctggcca ttcacatagt ttattgacaa
    48841 ctttacacta gcactttcaa tatatgaatc atcaaaatcc ttaatggtga tggtattgct
    48901 aattacttta tttactaatt gaggagtaac aagggctgtt gtttcaatgt tttcgagtat
    48961 tggggcatca ttgacagcta caatattgat gtttcgggtt tgtgtatttg aactacctga
    49021 gccatcacta atagtaaaag acactgtcct gaccgaagta gcaggatcat cagatgtatt
    49081 ctgatattta acagagcgta atgcggcttc gtagttagcc ttggtgtctg taccaactaa
    49141 atttaacgtg ccggttgcaa ggttccatgt acctgtaatt gtagcagtat tggtaaacga
    49201 aagtacgtct tgccctgaat tgtagttgcc cgtgattttt atagtagcat ttctaaaagt
    49261 agtattatct atatcagaaa tagttataga agaagttatt attttgggca attcgtcttc
    49321 tgtaaacgat aaggcgccag cttcaattcc ggcgagtacc ggggcattat tgacattggc
    49381 aatgacaata tttacagtca tattggaaga tgcataggct gagccatcgt taccattcca
    49441 ggtaaacgaa gtattgccgc tccaatcggc attagggaca aaagtaatat tggccaggtt
    49501 ggcagctata atttcctggt ttgctataat atttgctccc gacaatttaa gggtaccatt
    49561 ggccggaagt gtaactaccc ttatttttgc cagggcatca ttatcaatat ctgtgtaagc
    49621 atttgtaaaa tcagcagcag taaaggcaag cgtgatgttt tctgttccag cctttgaaaa
    49681 gtcagcaact accggaaggt catttaccgc acttatatta atggtaacat tggcagaaga
    49741 tgatgaataa gtaagcccat cataagcttt ccagctaaat gatgtagagc cattccaatt
    49801 tgcatttgga gcaaaggtaa tattaccaag gtttgtagct gttatttcct ggaaagccga
    49861 aatagcaaca cctgaaagta aaagggtacc gttgcctggc aatgtttcaa tccttatttt
    49921 attcagggca tcagcatttg catcgctata aactcctaaa aaatcagtag ccgcaaaact
    49981 caattcattg tcttctgtcc cgttttttat aaaccctgaa attacaggcg ctacattagt
    50041 attgctgata acaaatttta cttgggcccc tgtcagagca taagaagtac catcataagc
    50101 attccagtta aattcatctg taccactata tcccgtgttg ggctgataag ttaaaaggcc
    50161 caggtttgct gacattattt cgtaaaataa gcctaccggg ctacctccat aatatagggt
    50221 accttttgca ggaaggctgt ttatctggac cttttcaagc gggttagcat cagcatcgaa
    50281 gtagcttgcc gtaaagtctg atgttgcaaa tgaagttgca gcatttacag ccgatgattt
    50341 atatatatcc gaaagtacag gcttatcgtt gacaggggta actgttaacc aaattttttt
    50401 acttgttgaa gcgtacaatg taccatctga tgagttataa tcgcatgtaa tggcaccgtt
    50461 ccaattggca tccggaacat agataaggtt gcccaactga gcaaatgata tttcctgacc
    50521 agctgtaaca gctacaccac tcagcttaag tactccgtaa ggaggcaatg ttataatctt
    50581 tattttattg agggcatcat tgtcagggtc tgaataagag tttgtaaaat cagatggagc
    50641 aaatgtgatg aaattatctt cagcacacaa catgttgaca tcattgataa ccgggttatc
    50701 gttgaccggg gttaccgcta cattaatgat ttttacatct tctcccagcc catcgcttga
    50761 ttttacctgc acagtagcgg ttcctgagta attactttca ggagtaaaaa cggcattatt
    50821 caaagcatta tttacatcaa caagagaacc actgaaaatc attgaggcat ctgagttgcc
    50881 atcacctgaa gtaaatgtaa ggcctgtagt cccggacaat gttattgtgc ctcctgtaac
    50941 attaatggtt actgtttggg catcattgtc gtaatcggta actaaaatct ggttgctgct
    51001 tagtgaagta aaagttaatg gctggtcttc cagtgtaaca tcagtagtta acaaatgaat
    51061 aattggaaca aaatggcaat ccacaatttt tatcgggcca taaatattgt ttgaatagtt
    51121 acattcaccg taaggactgt ttgggataga gatagggtta gaaattacct gatttggtgc
    51181 taatccatcg gcatcatcat taacaacaaa atataagttc ccatcattag ccccgtttga
    51241 gtttaccaag tcgctaaggg aaacggttat aggctctgtt ttatagcatt cactggcatt
    51301 gagggtaact gttatcattg attttttgag aaaaatagcc ccggcatttc ttgggtcgtt
    51361 ttggtaaaaa gacatgggaa tagtataatt tgccaatgca tcgcccctgt tgcaaatttt
    51421 taacctaata ataaccttat caggacaaga agaagaaata tctacatctg atgtaacatc
    51481 ttcaacttca actgtaaagt ctggcccggg aaaatattta tctccgctct ggtctattct
    51541 gtatagctgg gtaaggtagt tgttcaggta tgggacaaga tggttctttt gttgtacctt
    51601 aggaacagtc aggtcattat tgatgttaac atagttatag gccctttggt taaatacctt
    51661 tcgggccgaa atccatggtg tacggttgtc tcgatataca cgtatgcctt ctgtacatga
    51721 tgctatgatt tctgcacggt tgtcatcatc aatatcagca ataaccgcat gttcgccacc
    51781 agtaccacta ccacaatggt tggcatcctg aaacaagatt ttggcattgc ctgcaccatc
    51841 tcccacccca acataaactt taagcgtatc ttggtcgcga taaacaagct caactttccc
    51901 gtcgccttca aaatcaaaaa aattcatggc tgtttcgcct gactggtctg tggttgcaga
    51961 ttgccacata cgttgcagag ctgtacctgc atcgtttacc ctgatcatct caaacctgcg
    52021 ggttgaaact atccccatct ctggcctgtt atcaggcaaa ccatcaacat caccaataca
    52081 ggcaacacct ccatcgtgct tgcctgctgt caggttatcc cctgccaata caaatgtagg
    52141 gtacaatcgt tggtttgtca aagggttcca gacactaagc ctgccttggc caacagttat
    52201 aatatccata accccgtcca tgttaatatc agccaatgag ttcttttgtc cgccatcatt
    52261 atcttgctga ttagccgggt tagtagcatc tccatgattg tatgaccatg ggaaatggtc
    52321 gcttgctgaa cctgttgacg taagaggatc tactccgtaa acatcttcta tatttccact
    52381 tgcaatatct actgtgtaaa tccggttccc ggcaataatc tcagggccat tggcgtattg
    52441 taaagctgtt ttggggcctg ctccatccgg gtcaaagccc gttggaataa catcagcaaa
    52501 agcaaaaaca tctttccagt acaacctgtt cctgaaagta gcattggtgg tcaaatcaac
    52561 aagtgttgcg ccatcagatg cccttaacac ctttgctgta cgcgtagcat cagtagtcca
    52621 tccatatact actaattcag aagtaccatc ttcattaaaa tcaacaaagt caaccgggaa
    52681 ccctcgtttt tgatatatat tggaaattat ccaggtctgg ttccaggcaa tgccatcaaa
    52741 atcataacgt atcaatgaat tggcattggt tgcacaacca tcattaccag ttgtcacaaa
    52801 aatttcagca taaggatcgg catcaacatt ggcaaagctt atgcctccgt ggttgccgct
    52861 tatgtctctg gctatataat atttaggttc gcaattagac gggtcaaaaa tatatacccc
    52921 ctcgtctccg gtacctccat aaccatcccc tactgcaata atttcaactt tgccatctcc
    52981 atcaacatct ccaaccttcg gggtatcata accataaggg gtaaagtttc ggatcacccc
    53041 tcctaccata aaacctccct tcaaataagc acattctaat tttgtggcaa attctgcatt
    53101 gcctgggtcg gcagtaattt cgcattgtgc cgaaggcaag gcacaatatg gcgatgtata
    53161 gcattggtta tcaaagcagt ctatcatacc atcagcatca tcgtctatac cattattgca
    53221 tatttcttgt gcgtaaatac tttttgatat caagaataat gttgccatga ttatggctat
    53281 tctgctgatt ttttcaaata cccttttcat ctttgacatt gtttgaaata tttggcagtc
    53341 tcgtagttgg ttacttatca ttttgtttat ttttttcttt tgtataatgc ccttcttttt
    53401 ctaaatgctc aatgctttct atcatttttt tcaaagcttc tgtaagttca gccgaagagc
    53461 tgatcaacct attgttatgt ttgcctttca tttgcataat ggtatatttg acacaaagtt
    53521 agaccctgca agtgctacat tgcttgttag ttatctctac aatatctcta cattgcctca
    53581 aggacatctc tactatttct ctacaataac gttattgtga aacataaaaa cgcctgtcaa
    53641 aacattgtca gacaggcgtt taaatagtat taaagccaat ttacggcaag ctttttattt
    53701 gtattaaaaa acactcaagc gattaagaaa ttgtaattca atgatttaac aaaattttca
    53761 aacaatttct aaatcagcct gtcccgttta tatcgagagg gctaacccag ctaaatcata
    53821 ttgttcggct ttaaaaagcc tcctaatgat taatttggtt gaaaactaac ttttttgatt
    53881 ttaatttttt aaaaattata ttgattgcag atactctacc aaactatctt cctgctgaag
    53941 tcccagcttt tttcttaacc tgtacctcgc catatcaaca ctctttgcat cctgcagggt
    54001 aagggcggca atatctttag agctcaaatt gagtttaagt aaagcacaca gttttttctc
    54061 atttggcgtg agttccgggt gaagcttaac gagccgatga tagaagtcct cataaacctg
    54121 cccaaaacgg gtctcaaatt cttcccacat tgaattatag accttaatat tatatttgct
    54181 tttaaattct gctattagct ttttgccttc ctcatctgta tgtttatcaa gaatttcaag
    54241 gtcttctaat agtttgttgt tcatagtgct atactgcatc aaccgcatgg ccgttgatac
    54301 taattccttt tttttctctt caaggttgcg ggcatgctct tgttttattt ctgatatatc
    54361 tgataaaagg catatcagcc cttctggttt tttgtcagga gagtaaaaaa tagtttttgt
    54421 aactaaaaat gtagcatctt ctttattcac tgttttcatt acagtttcaa aagaggtttg
    54481 ttgtaacccc ttgtttatca tatcttcact ttgcgctttc atctgttcag ggttggggca
    54541 aatagtgtac acttctgtac caataaaatt agacgaaatg tgactaaacg ttttctgaaa
    54601 actctcattg caacctaaaa ctatgccgtc tttgttataa taaaccattg gggagggaat
    54661 agctaaaagc aatgaattta taaaacggtt ataatcagat aataccaggt tataattttt
    54721 tttatccttt attgatttag aaacaataag catggctttt gtccttgcca gtaactctgt
    54781 tttatcaaaa ggtttacgca aaaagtcaga agccccgcat ctaaatgctt ccttaagatc
    54841 ttcaggagta accatcatcc ctgtaatcat aataacaggt atatcacaaa atgtctcatc
    54901 ctgtttcatt ttacttatta gctctaaccc gctcattgcg ggcatatccc aatcagtaat
    54961 gaccaggtcg ggagtccatt ggcccatggt ttcataagcc tgtttgccat ttaatgcttc
    55021 tcgaatctcg tacttttcgc cagatgtaag caatgattta cttgtaatca ttaacatttc
    55081 aggcaaatca tctacgatta atattcgtgg aacattcata aaataaggtt ttattaatta
    55141 ttgtttaatg gcagtagtac attgaacgta ctgccctttt ccgggatact ttctgcccat
    55201 atttggcctt tgtgcaatgc aacaaattcg gcacaaagca ctaaacctaa acctgtaccc
    55261 ctttcacttg ctgtgcctga ttttgaaaaa ctggtctcaa atttaaacaa ccgggataaa
    55321 tcttcctggc ttagcccaac cccgcaatct tgaatagata tcttgatatt gccggtttct
    55381 tcaatgattt gaagaaatac ttctgtatgg gtataagaat attttattgc attggtcatc
    55441 aggttgcgta ctattgtctt caacatttga ctatcggcat aagcttttag accggggctg
    55501 atggttttct ttatggttat ttttttctga ctggcaagca attcaatggt atttaagtca
    55561 tctttcaagg cttcggcaac atcaatccat tcggggttga atctaacccc ttgcctttga
    55621 aaattgctcc attctaacag gttttgtaaa agtgtatgtg tgtttttggc tgtttcataa
    55681 agcatctcgg cacactttcc tgcatcttca tagttgttat cagcaatatc ttgttgcacc
    55741 atctctgaaa ggttaattag cacatgaaac ggattgcgca ggtcatgggc aattatcccc
    55801 ataaatttat ccttggtggc atttaactct tttaattttt ttgcattttc ttttaaggct
    55861 acttcgttga gcttacgctg ggtaatgtca gataagatta tggcccaacc ggccagtctg
    55921 tctttgtttt caaaaacggg agttgttttt atcaaaaaat atcgtcgtcc tccaaaacaa
    55981 tcattttcaa tatcagtaga atactcttcg tgcctaaaag caattcgttt ggtatcctcg
    56041 cattgttcaa gaatcttttc tatgcttttc ccaatgcttt tttcgtttag gttgttaaaa
    56101 atttgttttg cgtgattgtt gaaatttgta atcctaagct ttttatctac tattattacc
    56161 ccatctgaca tgctttcaaa aataacttct aatgctaatg gctcaaaatc aagcagctta
    56221 aaacgaaaca tgcctaaagc ccccattata cctacaattg aaaatgaaaa tggaataata
    56281 tccatattac ttttgtagta tgatgaaaga taatatatat agaaacccca tgggatagta
    56341 agtgaaacta tcatcaaaaa tgcccttacg cggttgatgc caacttgttt tatggccatc
    56401 catacatata aaagaaaact aaaaagcagc gttaaatttg aatatacctg gtgtacccaa
    56461 taccaaggcc cttttgtaaa gttggcaaat gaagatgatt gtgcggtatt gatggccaat
    56521 tttgtataaa aaaggtggcc aaaattacta ttctgaagaa ctattgttgt acaggaaatt
    56581 gcaatcatta ccactatcat ccattttttc aggtactgat tatagcctgt ataatgaagc
    56641 gtaaatatga ccagtacaga agggaataaa gcaattccaa catattctac tttcaaccaa
    56701 aaatagatgt cttccaaatt gtcagaattg agttccataa cataaccaaa agcatacaat
    56761 gctatgcata ataaaataga caaatatgcc aatgcccctg gtgacttgta ataccttata
    56821 gtgtaaaatg ctatcagcag gttaatgaaa gaggtaagca gcaatatcag gctaagagga
    56881 tgcataaata ttggttttgg tctgtttcaa aaacaaaatt aataaatcct aagctacttt
    56941 tataacagat ttattgtgtt aattggcctc tgtggcaata attttggccg atgcaagaca
    57001 atgatgtatc cctcctccgg gatgtacact gctgcctgtg aaatataaat tttcggtttc
    57061 tctcgaaata aagttcgggt gttttgtaaa aaatgaccta aaactattta tggcctggcc
    57121 aaatagcgaa ccgttatgtg tattgtatat attagtatag gttaatggag ttgctacctg
    57181 ttcaaatagt atattttttg atatattttc tccaaatgtt ttttcaatct ttgataacac
    57241 aatattttta atttcttccg taactttttt ccagttgtgc ccgtgcttag caggcacact
    57301 aaccattact tgcaagtatt gataaccttc aggattatta ttgccagata aaccactaat
    57361 atcattgata tatatagatg ggtcactgtc aatagccttg tttttaaata gtgtatcaaa
    57421 ttgttttttg ctatcgcccg atactattat attataaaag tttaaccctt caattttctt
    57481 gcttaacccc caataaaata tgatggccga tgaagttagt tttttcttca tggctttttt
    57541 ggccatcttt ttatcttgca taaggtcgcg gtaaaagtgt actacatctg tatcaataac
    57601 aagaaggtct gcaaagtaag cattagtggc agtgttgata gatacaatac gcttattgct
    57661 ctggaaaacc cgggttattg ggctgtcaaa atagaatgta acgccggctt ctatggccaa
    57721 atcatacaaa gcctttacaa tactgtacat cccgttttgt acatgatata cccccaaatt
    57781 atagtttaaa tgcgaaacat atttgatggt tgcaacagat ttataagggt tattgccaaa
    57841 tgatgaagct gcactattaa atatttcaac aaccttgctg tctaaaaata atttgcggtt
    57901 tcttttatcc agtgaagtaa aaatatcaag cctgtaaaac tgcaataaag ctttacggca
    57961 aggggctttt aaaaaatttt tgcacaaata caaagggcta tataaaaaca atggagatgc
    58021 aagctcccat aatttttgac atttgtgaag atattggtaa actttgtgtt taggtactga
    58081 tgttttgttg cttatctcgt ctgcaagttt ttcagcattg caccatgcat tgatttgtgt
    58141 cccgtcgggg aaaaaatata gcccgctgct ttcggcacga tggtagttga aataatttct
    58201 ggggtttttg ttgtgaagga caaatagttc gtccagcatt tcaggcatat atagttttgt
    58261 aggcccggta tcaaagcgaa aaccattttc ttcagcctga ctaatccgtc ccccgggcat
    58321 atcgtttttt tcaaatactt ttactttata ccctttggct gcaagcctta tagccacggc
    58381 catccctcca atacccgaac caatgatgca agcggttttt gtcatgttct tcattctaat
    58441 tgcaacaaat atagcaaatt aaaaatccga aacaaatgct aactgtttta ttctgaaacg
    58501 cttaccctta aacctgcttt ttcgatagtt gaagcaatgg ctttcaattg ttttccgctt
    58561 attttttcta ttgtttcaac aggtgtatct cttgctatag tataaatcat aaccagctca
    58621 ggagatatgc tcttcaaagc atctgtccag gctataattt cagtatctgt ggtattgtca
    58681 aatgcttgtc cattaaagct tcctttgaca aacatagttt gaacaaccag ttttccattg
    58741 aattttttca gattttcaat aacgtcattt gctgtaatag gtaattgagg ctgattaagt
    58801 atttgaaatg tcctatttat agccgaatca agtttcatta tgttcaaatc tgctttcttt
    58861 aaagcattta acacactttg cttgtgcaaa agcaacgaat tggaaagtac tgcaattttt
    58921 acttgtggaa agatgttatt tcttacagtg attacagcat ctataacttc ttcaaattga
    58981 gggtgcatag taggttcgcc gttgcctgca aaagtaatgg tgtcaacagg ctcaccttta
    59041 cttttcattt cattgaggcg cctttgcaac aaattgctta tttcaccaac agttggaagg
    59101 tataaatctt ttttgccttt tgtattgagc ccacattcgc agtaaatgca attgaaattg
    59161 catactttag catctgtcgg caacaggttt acccctaatg aatggcctaa cctgcggctt
    59221 tttaccgggc caaaaatgat tttgtcaaaa agaaaggtag gcatgattgg gaagatttat
    59281 taaagatgtt tattgaatgt tatgcaggta atgcaagcat tatccccata aaatttattt
    59341 catgtagttt aaaagttctt ttaactattg caggtaaaaa taaacaaaaa agctgccaga
    59401 gtttattctg acagctttga aatcgaaaat tttacaaaaa tgctattctt tgaccctttc
    59461 acagtagcta tagtcgcgag tatctatctt tatcttttct ccctggttga caaacaatgg
    59521 cacattgata cgtgctcctg tttcaacagt tgcaggtttt aaagccgttg atgatgcagt
    59581 gtcgcctttt aagcctggct ctgtataagt aacttcaagc actacaaaag gaggcaattc
    59641 gcaacttagc ggtgtttcat tatcagcatc aaaaactatt tcaacatact gtccttcttt
    59701 catcaggtca gcattttcaa tcatgttttt cgggatagat acttgctcgt atgtttcgtt
    59761 atgcatgaag gtatatccat actcatcact aaaaagatat gaatgtggcc tgcgttcaac
    59821 atgagccaaa tcaacttttt cgccagcagt aaaagtgttt tcaattattt taccgttggt
    59881 aaggcttttg agctttgtac gtacaaatgc cccacctttg cccggcttta catgctgaaa
    59941 ttgtataatg gtgtaaatat cgttgttgaa taatatacac aaaccatttt tgaaatctgc
    60001 tgtacttgcc atattatcaa tgtattaatt attaaaaaaa gagtggcaaa gatatacttt
    60061 ttcatggaaa ctgaaaaaac aggaattata tttttaaaca atgttgtccc gtaaaataaa
    60121 atagatactt ataaatactg gtaatattgt tgattagaaa ctgggccccg ccggggctta
    60181 attttaacga tgtttaattt tactacaaac aggtcatcgc tacgcgacta tataatagca
    60241 gcgcagcagc gaactgtttg taggataacc aaatacacca catgtttcaa agccacatag
    60301 gggcaaccag tttttgccgg gcaacaatgg tttttaaaac atattaatca atagtcgtgt
    60361 aacatcttgg tattttctgc ttcttctaaa ataattttta attcgtaatt gaaaaatcct
    60421 taattgtcat atcatccctt catctgcaaa actaaaataa gccttgtctg taactataag
    60481 atggtcaaaa agttttatat ccatcaatgc gccagcttct tttacttttt ttgtgatatc
    60541 aatatcttgc tggctgggct ttgtattgcc cgaaggatga ttgtggcaca tgattactga
    60601 gctggcttgt tttataattg cattgcgaag tacaatacgc acatctacaa ctgtacccga
    60661 catgcctccc tggctgattt ttaccggctc aatcatcctg tttgccctgt tgagcaatag
    60721 tatccaaaac tcctcataag gtaagtccct gagtttgttt ccaaaataag tataaacatc
    60781 atggcttgag gcaaaggttt ttgcctcttc gtatggttgt acgctacgcc ttttgccaag
    60841 ctcaagagct gtatgcacac tgatggcttt ggcttctcca atgcctttta tctgcatcaa
    60901 ctggtcaagg cccattttgg caagacggtc gaggttgtta tctgccattt ccaacaattg
    60961 acgggccaat tctactgccg acatctcgcg gctaccggta ctaaaaagta ttgctaatag
    61021 ttctgcatta gacaaagtgc ctatgccttt gcgcatcatc ttttcacgcg ggcggtcgtc
    61081 ttccgcccag ttttttatac tgttgtttgg tttcatattg gggggtaaaa atttcaatac
    61141 gataaatata atacgataag ttaaaataac agtggtaata tatcgtcagg caaaatggtt
    61201 tggctccgtt tgatgccgaa ataaacggat tatacttgta atttgcaatt gagaattaat
    61261 caaagttcta ccagcttgtg gtttactgca taaacggcca gcgccaccga attcttggtt
    61321 tgtgtctttt ccagtaagcg tgcccggtgt gcttctatcg tccggctgct taaatgcatc
    61381 aaatcagcta tttgtttatt ggtcatcccc tgacaaatat aggtaagcag ctctttttct
    61441 tcgtccgaaa aagtttcttc gggttgttgg tgccgttttt tctgtatctc gttgaaacgt
    61501 ttagaaagct ttacaatcag ctcatccgaa tagtaggtat aaccttccat aacccgctca
    61561 atggctttta taaagtcgtc acgcaacgaa ttcttcaaca aatagccttt tactcccaaa
    61621 tctgccattt tgataagata ttcttcatcc tcaaatgagg taagtgctat aatcttcaat
    61681 tcggggaaaa gttcgagggc tttttcagta gcttcaatgc cgctcatttc tttcatgttg
    61741 aggtccataa aaatgatatc tggcatcgat tcgtttaaca actcaagaaa aaccttgcca
    61801 ttgggggctt cgcctatgac ttttacattt tttaccgtat tcaggattaa ctttagccct
    61861 tccctgaaaa ttttatggtc ttcaacaata aatactttta ttggatggat catttgtaac
    61921 ttttttttat tggcacgaaa aatacaaaaa aagcaattgg ggttcaatat taagttaaac
    61981 aaaaaaaaga gcatatttta tcattgaagg ctttaaaaaa tcgaactatg ataattaagg
    62041 cgctataacc ttagtttaca ggcaacaaca atttcgtttt tatcagaccc atcaatagca
    62101 tatcctccct ggcctaaagt agtacggtca aaatagtgag ttgatgaata atggatttga
    62161 gcattcattc ttttgctgag ttttaatttt cctgacacta catagcgtaa tcccttccca
    62221 taataaactg gaattgacat gtatgaacca gctcctcttt ctattgcata tattcgggca
    62281 tcatagctgt cggtattgaa ccaggccagg cggccttgca gataaaatct ttcttgcatt
    62341 ggcttccaac atatatcctg caacatagca aatccagatt gtttgctttg gccgtcctcg
    62401 ttgtaccaaa cccattctgc cctgttggta atgcttatcc attcattggg cgaatatttt
    62461 gcttctaatc ttgccctttg tgtggtataa tctatgatat gctttacagg tattgtgcct
    62521 gaactgtctt gcatcccata tttcgctgtg tatctggcag acaagttaag cccattgtca
    62581 aatatccttt ttacctgaaa ataataagtt gaactatacg aaggttcgct ggtgcgatat
    62641 tttaaccaag gatagcttgt tatatctgca aacaaagaaa caaagtactt agtaaatggt
    62701 tgtatgtcaa ttccggcata ccatccatgt tcgttgacat tcagcgactg ctgtccaaaa
    62761 gcgttggcat aaggggcata gtaactttta tcgtagtagc ggtacatgag cgagaagcat
    62821 atctccgagg caggatttac agctattcca tgaagcaatg ccaacgaata gtttttagcc
    62881 atggccgttt cgccgtaaaa gcggatacgc cgggtattta cgcggtaata ggtgctggca
    62941 ttgctgatgc tgttgcctgt gaaagcaaaa cgtttgtaaa aatcatcact ggcaggtttt
    63001 acagcatcgt attttgttgc agtaccaagg gcaccaagca ctatgtattc attttgccac
    63061 aaaatgttta ctccgcctgt actttcatgc agtgcatgct tcttctccat atcagcaggc
    63121 aaagcatgaa tgccagtatt aatgaaagaa ccaaagcttt tttcggtatt cagtgaatca
    63181 gtaagaatta t
//
LOCUS       DAOSPF010000013        56242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_618_length_56242_cov_7.102034, whole genome shotgun sequence.
ACCESSION   DAOSPF010000013 DAOSPF010000000
VERSION     DAOSPF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_56242_cov_7.102034"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1282)
                     /locus_tag="PK252_04170"
     CDS             complement(113..1282)
                     /locus_tag="PK252_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT13938.1"
                     /translation="MEDNSRYKMRGVSSGKEEVHQAIKNIDKGLYPKAFCKIIPDIIG
                     GDPAYCNIMHADGAGTKSSLAYAYWRETGDLSVWKGIAQDALIMNIDDLLCVGATDNI
                     LVSSTIGRNKLLIPGEVISAIIQGTEELCAELRNVGIGVYPTGGETADVGDLVRTIIV
                     DSTVTCRMKRSDVIDNANIKAGDVIVGLSSTGVASYETSYNGGMGSNGLTSARHDVFE
                     KYLAKLYPETFDAAVPNDLVYSGKYKLTDPSPVEGVNVGKLVLSPTRTYAPVVKAVFD
                     KLRPAIHGMVHCSGGAQTKVMHFVDNLHVIKDNLFPTPPLFEIIQQQSKTDWKEMYKV
                     FNMGHRLEFYTDAATAKEIISISEKFNIEAKIIGRCEAHQGKKLTLETVNGKFEY"
     gene            complement(1300..2544)
                     /locus_tag="PK252_04175"
     CDS             complement(1300..2544)
                     /locus_tag="PK252_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT13939.1"
                     /translation="MSFEYFIAKRQLKGNDKKRKISGSLVNVTIFGVALCIAVMIISI
                     AILQGFKNEIRNKVVGFGSHIQILNYDSNSSYETTPISNKQEFIADLKNAEGIKHVQQ
                     FATKAGILKTETDIQGVVLKGIAPDFSWDFFRQNMVVGKTFTVKDSEKTNEIIISRYI
                     AKLLKLKVGDEISMYFIQDPPRVRVFTISGIYETSVEEFDKIIVLCDIKHIQKLNNWT
                     DDQISGFEVFVNKFDDIDKMTVVVNDIVGYSFMENGQALQVLSIKQKYPQIFDWVNLQ
                     DLNVMIIIILMLVVCGFNMIAGLLILILDRTELIGLLKALGSTNKKIRRIFLFESVFI
                     LFKGLFWGNLVGLAICLGQQYTRFLKLDASSYYIDHVPILLDPWYIVLLNVGVAIATL
                     LFLIIPSSIISRFSPAEIIRFK"
     gene            2731..3768
                     /locus_tag="PK252_04180"
     CDS             2731..3768
                     /locus_tag="PK252_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT13940.1"
                     /translation="MLKEKKVGLVANHTSVVKDAHLLDTLLSLKIDVSRVFAPEHGFR
                     GETEAGERVKNEVDAKTGIKVVSLYGRHYKPTKQDMDKLDIVVFDIQDVGVRCYTYLS
                     TLHYVMEACARYNKQLIVLDRPNPNSFYVDGPVLELKYKSFVGLHPVPLVYGMTIGEY
                     AMMINGEGWLPEALKCQLTVVPCKNYTHADKYILPIKPSPNLPNMQAIYLYPSLVLFE
                     GTCFNVGRGTDFPFQTYGHPQFELKNFSYTPRCKKGTMTLHCDTLCYGEDLRNVSCKG
                     FTLDYVINAYNRYPYKQEFFNRFFANLTGNTWVKEQVVNGKEVSEIQKMWKKDLVFFK
                     DSVRVKYLIYK"
     gene            3880..5265
                     /locus_tag="PK252_04185"
     CDS             3880..5265
                     /locus_tag="PK252_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT13941.1"
                     /translation="MIRKKLYFLVLVPLLFSTCNFNRNNLATEADLPEIQKRGRIVAL
                     TNYNTSDYFVYNGNPMGFQFELLRQFAEYSGYQLDIVVSNNMADAMQMLIDGKCDIIA
                     QNIAPTPEHLLYASFTFPILQGKQVLVQQNKSESPGRYISDLNQLKNKTVIVSSGTAF
                     AQRLKNLETEIAGKIDVVEVPEDEEQLIRFVAGGEIDYTVCDEYTARLNKQYFPKLDI
                     NVEVSIPQNMSWAVRNNSPKLLELLNRFIEAEKKTSRIAVLYNKYYQNQWAQGMVNSK
                     YFVLNSGQLSPYDELLMSCSAELQWDWRLLAAIIYKESQFIPNIKSHRGAVGLMQLMP
                     STAGLMGYDSTQVEDPSKNIIIGVKYLKWLDNKISSLIPVKEERIKFVLAAYNIGLGH
                     IFDAIELAKKYKKDATKWDTVKDFLLNKAMPEYYRDAVVKFGPCVGKQTVTYVSEVLS
                     CYSHYKNLTPQ"
     gene            complement(5271..6692)
                     /locus_tag="PK252_04190"
     CDS             complement(5271..6692)
                     /locus_tag="PK252_04190"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOT13942.1"
                     /translation="MFMLLTVFQAKAQDNYTLYFMHTLPQANILNPAVQIPCKVYVGI
                     PALSSIHVGYSNTLFRYKDIITTNQSGNSVFKPDYLINASGDLENIRLETQISLIHFG
                     FFYKDIYFNFHLQDKIDVGLNYSGNLISLTLQGNAPHIGETFDVGGTALHGIYYREWG
                     IGASKKIDNQWTLGIHAKLLFGKVNIQTRKKDLALTTTSDSLQLSASGHYLIKASPFV
                     ITTDAQNQPTSIELVDASIVSLLLNRQNPGISFDFGTLYQLDDNTTVAASLLDIGGIY
                     WRYSPAQVDISGSYNFNGIQYDPLTGTFNNLDSVVNAAQDAYSYSTSQSSYFTFLTPK
                     LYLGASYDLNDWANASLTYRSEYYHGKILQGISPAINAKYKNFLAGSLSWTFTRGTLM
                     NLGAGISARTPTFGFYAIADNVYGMIKWKSTKFVNLRFGFNLLFGCSSGKSKGSSSAA
                     CSAYSNTLKKKQRLEELKSKNGK"
     gene            complement(6725..7141)
                     /locus_tag="PK252_04195"
     CDS             complement(6725..7141)
                     /locus_tag="PK252_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13943.1"
                     /translation="MGDNVPIDIFKFDQNNKYIKSLTLRIFSSNQFPANAALFVTLDT
                     SNNQSTLGSSFLSKIDIDAAQYNEDLTIKEEAKNRTDITLDKQQIDSWLKAQYIHLYC
                     AVNNQIKKNEQYKYYETYKVNLYIAVIIEFDYLISE"
     gene            complement(7119..7364)
                     /locus_tag="PK252_04200"
     CDS             complement(7119..7364)
                     /locus_tag="PK252_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13944.1"
                     /translation="MKKIPLFFIAFIMFAFAACDEPDDLSGLKTEHQYQGTWAFPLGN
                     TYLNLKIMGINMPEAWKEHPEYLQIPDTIIYGRQRTH"
     gene            7663..8706
                     /locus_tag="PK252_04205"
     CDS             7663..8706
                     /locus_tag="PK252_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13945.1"
                     /translation="MMKHIYSLIAGFILFSANLYSCTVFSVSNADTALFGNSEAWLPG
                     SKTRYWVIPASDNSFGRICFGYQGEALAQGGMNEAGLAFDWVASLESSGYELNDTLIT
                     LETSLSEKILAEASTVDEAIKYYEKYNEPYFSNAQILLADKNGDAAIVGWQNGKLKVE
                     KKNGKAIVLGYEDTVAFNFLKNIEPINPQNCATILAKVLQRGIIATCYSNIFNLKTGE
                     INVYNFLETSNSIRLNLKEELAKGAHSVNLNEALSSVKIKVAEANDTKNKVYPTICKD
                     SFTLEMRDVNGKILDVFIIDMFGRKQMHFIENVVGSNVFTKTFHISNLKEGNYLVIVY
                     DGMNQSQYKVVKY"
     gene            8828..9403
                     /locus_tag="PK252_04210"
     CDS             8828..9403
                     /locus_tag="PK252_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT13946.1"
                     /translation="MIEVSIKNMLGDYQYNPADIEIIASKFYPETIKKGAVFCERGKR
                     INKVGILSKGLLLAYYENYQDEIEVSRYFDTVVNGIVVDFDAFVNQKPCSETIKAIED
                     SEMLCITRQNLYQLYKEVPAMNIIGRRLAEEGYVLAVRRIQALQTLDTEERFRRLYLA
                     NPTLFNRISKKDMATFLKMNRNLITQYLKKV"
     gene            9478..10224
                     /locus_tag="PK252_04215"
     CDS             9478..10224
                     /locus_tag="PK252_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT13947.1"
                     /translation="MKNTFYIMCICVCTTLLAQCQSIPTTNIMEDEVYIKCFPTGIYN
                     SNCYVVSKDGLGFMVDAGAPDTTVLQYIRENNIKIKYIFCTHSHIDHVLFAPEAKQYT
                     GGKIVLHANDEDHYKYYTSERIGEWMKSGQVTEQQMPYIEKFIHIKYDTLVHGDEEFA
                     IGKLKVKILYTPGHSKGSMCIFLNEKYLFTGDAVFNDNVGNAEISSGNISDLEKYIKE
                     EILVLSDDVVLFQGHGGKMLLKDLLPKISF"
     gene            10443..11588
                     /gene="nspC"
                     /locus_tag="PK252_04220"
     CDS             10443..11588
                     /gene="nspC"
                     /locus_tag="PK252_04220"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOT13948.1"
                     /translation="MAIDFNKVPSPCYVLDEALLRRNLQIISNVRDKAGVEIIVALKG
                     FAMWSAFPILREYGFKHATASSLNEATLANEIMGTPAHTYCPAYSPDEFDEIVGLSSH
                     ITFNSLSQFEFFKNKIKSANENVSVGLRINPGYSEVKTMLYNPCAPGARLGIPAQTIG
                     NSLPKGIDGLHFHTLCESSSYDLEKTLTVVEERFGHLFGQIKWINMGGGHLMTRKDYD
                     TEHLIKLLQTFKQRYPHLHVILEPGAVFAWETGYLVSTVLDVVENDGIKTAMLDVSFS
                     AHMPDCLEMPYKPVILGATDEEEGKPTYRMGGNSCLSGDYIGSWSFNNPLKVGDKIVF
                     NDMIHYTMVKTTTFNGVRHPSIGIWNDKQGFRLVRRFGYEDYKNKLS"
     gene            complement(11700..12347)
                     /locus_tag="PK252_04225"
     CDS             complement(11700..12347)
                     /locus_tag="PK252_04225"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HOT13949.1"
                     /translation="MKTHRLLFVVISMVGLLSACEETDNETYYSTLGNLKITADSVII
                     ESDKGNRMMVKNSLSSSFKDNDRVIAYFTMAEQPLPKGIDYVINVYNIEKVLVKPVIV
                     YDSTKADSIGNDPIFVNGTWISKNYLNVDFRFYGYNLKHMINLTRNAGIFGADTIQLE
                     IRHNDKNDAPQYTLNGFVSFDLTTIEHPEKDSVVLHVTAKEYGNKVYEKYLTYRY"
     gene            12678..13541
                     /locus_tag="PK252_04230"
     CDS             12678..13541
                     /locus_tag="PK252_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13950.1"
                     /translation="MKYTIGLVLICLASIVQLYADTIPQRGIGFAAGYLRYSSIDCIV
                     NPYIYSGQNMSYSFSTFYRNQKHFRQFDFTFSFLNKEIAHVNIPDSYSIVGYDNNNTY
                     SVASNEWLKKVPVRFFVLQYSYSRRMGFFNSFQMGDFYWSLSNINQTIFKNDLSYMEL
                     IAFGLYPGLKYRHKLFEKYYVSCDVNILLASITIRRPYAGVDAQLSDVQNVQTFWSSV
                     KANNYFNLPNNHLDLRAQICLERAIGRRFSMFCKYLFEYSEVKRPRVLQNSYGAFNLG
                     IYYNFYRKINE"
     gene            13534..14625
                     /locus_tag="PK252_04235"
     CDS             13534..14625
                     /locus_tag="PK252_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT13951.1"
                     /translation="MNKGFLAILILFVFTSCNESLLNEDVENTPLGNFDALWDAYDKT
                     YGAFEAKNINWDSLKIVYRSKLSQNSTNTELYNVLSGLIQCLNDGHVQLTASGYKRFY
                     SSGAYTIFLDVKYYENGNMISVFYKLDFSKYINTLLADDNFVYGYMMAKIKSKKVGYL
                     LVPTFSYNKYRQDIVDKAFDEFKSNACAIIIDLRFNGGGATETFLKLLNMFADQKRLY
                     LKSKLRNGPKHSDFTQLYDHYLEPKYGTFDPKPVIVLVNRFSASSSEHFMLGMQTLPY
                     VTVVGDTTYGALSTVLENVLPNGWQFRTCPQVLCDTSGNYLRDSKGRYPDGVGLCPDV
                     YQPNYYSDILNGHDYMLERAMQIISEQVK"
     gene            complement(14670..15104)
                     /locus_tag="PK252_04240"
     CDS             complement(14670..15104)
                     /locus_tag="PK252_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13952.1"
                     /translation="MGLFDIFDSREKKKRLSHMFNLLAVACADGVLEQAEIDLLLQIG
                     KRMGVSYDELKRVIERPGSVKFTAPETNEERIALLYDMVLVMMVNGDIDEGEVLYCKN
                     VAQKLGFSHKIIDIMVNKVIELVKQGVENEKAVENLLTVAEN"
     gene            complement(15134..15979)
                     /gene="hisJ"
                     /locus_tag="PK252_04245"
     CDS             complement(15134..15979)
                     /gene="hisJ"
                     /locus_tag="PK252_04245"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HOT13953.1"
                     /translation="MQLSNYHSHTSLSDGHCLMEDIVSEAINCNLISMGFSEHSPVPF
                     YSDWNMKASDITNYINKAKELKVKYASQLEIYCGLELDYFEPYNKQILALSQVEKLDY
                     TIGSVHYMGFFADGEAFNIDNVLEKYEQGIVELFNNDPVLLYQKYYEAVIKMIEQMQP
                     AIIGHIDKIRMFNTNNRFFNESSEEYKKAWKQVLVAAKEKGCIVELNTRGMYKFSAEL
                     SYPSSELLHAMQRMDIPVTINSDCHKAEEIVKGFDLAFNLLKDAGYTEYMQLRNGKWV
                     GNKIL"
     gene            complement(16163..18298)
                     /gene="scpA"
                     /locus_tag="PK252_04250"
     CDS             complement(16163..18298)
                     /gene="scpA"
                     /locus_tag="PK252_04250"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOT13954.1"
                     /translation="MKPDFSKININEMPASGKKPETEANWLTAEQIKVKPVYTADDLE
                     KMEHLNYAAGLPPFLRGPYSTMYVMKPWTIRQYAGFSTAEESNAFYKRNLASGQKGLS
                     VAFDLATHRGYDADHERVVGDVGKAGVSICSVEDMKILFNGIPLNKMSVSMTMNGAVL
                     PVMAFYIVAALEQGAKLEELAGTIQNDILKEFMVRNTYIYPPDMSMRIIGDIFEFTSK
                     NMPKFNSISISGYHMQEAGATADIEMAYTLADGLEYLRTGIKAGLKIDDFAPRLSFFW
                     AIGMNHFMEIAKMRAARLLWAKIVKQFDPQNPKSLALRTHSQTSGWSLTEQDPFNNVA
                     RTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETGICKVVDPWA
                     GSYYIESLTHELAHKAWKLIEEVESLGGMSKAVESGIPKMRIEEAAARKQGRIDSGKE
                     TIVGVNKYRLEKEDPIDILDVDNAAVREAQIKRLQQVKQNRNNAEVKAALEAITEAAR
                     TGKGNLLALSVDAAQKRATLGEISDAIEKISGRYKAVIRTISGVYSAESQDDEDFKAA
                     REMCDKFAQMVGRQPRIMIAKMGQDGHDRGAKVVATGYADLGFDVDMGPLFQTPAEAA
                     KQAVENDVHVMGVSSLAAGHKTLVPQVIEELKKLGREDILVIAGGVIPAQDYDYLYKS
                     GVVAIFGPGTSVSKAGRKIMEILLETHHE"
     gene            complement(18344..18733)
                     /locus_tag="PK252_04255"
     CDS             complement(18344..18733)
                     /locus_tag="PK252_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOT13955.1"
                     /translation="MTQEDFNKLTQSVIASAIEVHKELGPGLLESVYEICLMEELKNR
                     NIKAINQVKLPVSYKGKELNKEFIIDILVENELVIELKSVEEILPVHEVQLLTYLRLA
                     DKKLGLLINFNVPALKDGIRRRINGYL"
     gene            complement(18758..20617)
                     /locus_tag="PK252_04260"
     CDS             complement(18758..20617)
                     /locus_tag="PK252_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT13956.1"
                     /translation="MEESQLFKEFPPISVEEWEKVIIADLKGADYDKKLIWKTIEGIN
                     VKPYYTAADTSGLAQKNAIPGTFPYIRGNKPNHNLWEICQDIKVYDVKTANSLALEAV
                     ERGATAINFIFCKEEITSEKWQTLTKDIKIGCICFSVKACSQIETLYQLIVDDAAKNG
                     YDINLMHGALQYDPVGMLLTKGAWVKSEKEDMAVAANLIEKAKTQLPKYKVITVNGAL
                     LQESGATIVQEMAYSLAIANEYISKITAISKFSAEDVIASIRFNMATSSNYFFEIAKI
                     RAFRLLWAKVAEAWTGNIDKAKAYIHCKTLQRNMTAFDPYVNMLRATTECMSAVIGGA
                     DSVSVRSFDEIYKKADSFSNRIARNVQIILKEEAYLGNVADPAAGSYYVESITSSLAT
                     EAWKLFQQIESEGGFLEAVKKGTIQSAIEAVAQKRLANISSRRETLLGTNQYPNFTEQ
                     QLANIDAEIYDEKQILSSSPVVKPLIRFREANEIENLRLTTERSGKRPKVFMLTIGNL
                     AMRLARSQFSCNFFAVGGFEVIDNNGFKTVAEGVEAAQKAKADIIVLCSSDDEYATFG
                     AEAIEAVGSKAILVIAGAPACMDELKAKGAKNFISVKSNVLDTLKEYQKMLNI"
     gene            20815..21015
                     /locus_tag="PK252_04265"
     CDS             20815..21015
                     /locus_tag="PK252_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13957.1"
                     /translation="MKVNYSAEKLKKMIEKAIEDHEITRDEYDMIIHIATEDGHIDDQ
                     EKALLAELNNMIENKMVKFVAK"
     gene            complement(21084..22652)
                     /locus_tag="PK252_04270"
     CDS             complement(21084..22652)
                     /locus_tag="PK252_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT13958.1"
                     /translation="MKTRTFLVSAAILMGTRLFAGDFVTNSNHSAQWARMLSRNAVID
                     ADATYYNPAALNFLPKNGLFVSLNNQVISQERTIKSTYPALNDGSYTGKIFVPAFPSI
                     YAGYKTEKFTISGAFFIAGGGGGATYDKGLPAFEYGLTDLVPGLAPQGVNGYRADIHF
                     EGTSVFYGYQLNFSYKINDQLSIAVGGRLVYASESYKGYLRNIEVYNYYKNPNNWTRA
                     DAIMQGAATSFTNAAKGSQALIDNGAGAMTFAQVEGAGYISATNRAELEGALTALGYP
                     AATPISSANAIFTGAAAQYGAKAMLLGDQEADTKRTGMGFAPVIAINFKPNDQLNIGL
                     KYTHQANIELEYDTKKDFITGFNTDGTPITKFPNGQKNRLDMPSTLSLGATYKPIEKL
                     LVSVSCDYFFDKQANWSGRQDSLDGNGIEAGIGFEYSLNEKLALSTGYMYSKPGIAAG
                     CQSDMGFELNAHSVAFGARYKLNEMIEINAGIEYSAYEDFDKNFTRNAIAYKETYGKH
                     TLIGAIGLNLYFGK"
     gene            22944..23675
                     /locus_tag="PK252_04275"
     CDS             22944..23675
                     /locus_tag="PK252_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13959.1"
                     /translation="MKNHLKFLVMAAFTAVFVFASCSKDDDDNNTSSTEYKAPSVAHQ
                     NYAVEIPEALAAKVQNDNYLMGLTGALQSVNYLAQACFAQFPIDTKELGNASSKSNSD
                     GSVTYSVSIQQGIFYLTYFYSSAKSSWTAEFQYTGTNSKTKIAYVEETGNAGKAEYYY
                     DSGKVALKYFWTDEKTGQKIVVTGYDEQGKVDLTWNIVSNDDKSGSMKAYDAANVLIY
                     DITWKADGTGSYTINSEDGTIKGTF"
     gene            complement(23725..24333)
                     /locus_tag="PK252_04280"
     CDS             complement(23725..24333)
                     /locus_tag="PK252_04280"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOT13960.1"
                     /translation="MRNFEKYIEDLAEKLKQPLPGISAQQKMSPSHRHLFQVTQELTK
                     AAVLILLFPENGQPHIVLIKRTDKDEPHGGQISLPGGRKDNADKTLEQTAIRECYEEL
                     GVPQESIHIIGALTPLVIPVSHYEVFPYIGRVESKPNWQVQKEEVAYCIEIPVADIYN
                     PHTIKKETKNYKGLNVEIPYFLYKNEKIWGATAMILSEFISL"
     gene            complement(24498..25097)
                     /gene="thrH"
                     /locus_tag="PK252_04285"
     CDS             complement(24498..25097)
                     /gene="thrH"
                     /locus_tag="PK252_04285"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOT13961.1"
                     /translation="MVFFCSDLEGVWVPEVWVNVARITGIEELKLTTRDISDYDVLMK
                     HRIKILKERNITLKDIQDVISKIEPLPGALEMLDKIRSKSQIAIVSDTFTEFASPLMK
                     QLKYPLLFCNYLSVDEKGMVCGYNLRQPDQKREVTKALKQLKYEVVAFGDSYNDISML
                     KEADHGILYCPPQKVIDDYPELPVVHNYKELESAISKYL"
     gene            complement(25103..26464)
                     /gene="thrC"
                     /locus_tag="PK252_04290"
     CDS             complement(25103..26464)
                     /gene="thrC"
                     /locus_tag="PK252_04290"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT13962.1"
                     /translation="MSKIEYYSTNHCSPSVSFREALIAGQAPDKGLYIPNYIPTLSKD
                     VIWGFSEKNYFEIAYTVINNFLQGEIPEADLFALAKDAYNFDVPLEQVYDRNYIMRLD
                     RGPTASFKDFAARMMGRMIQYFLKKDNRKMLILTATSGDTGSAIANAFYGLDNIEVVI
                     LFPKTEVTDRQRKQMTTLHKNISVIALDGKFDDCQAMVKKAFADKDLQKLDLSSANSI
                     NIGRLIPQSVYYFWAWSQLASNADDKIIFSVPSGNFGDLMGGILAKHMGLPVEKFVIA
                     TNENDEVPVYFRSGSYKTIEPSRNCISSAMNVGHPSNMARIITLYGGQMDEKGNILKE
                     PDVAKLKNDMTAFSVTDEATRQTIREAYNSYNLLLEPHGAVGWKGLHDFYAENPSYKN
                     PYQLTVTLETAHPAKFPQEIKSILNIEPELPASLSQLDNKTEEMTDMPNNYEAFKTYL
                     LKR"
     gene            26646..27839
                     /locus_tag="PK252_04295"
     CDS             26646..27839
                     /locus_tag="PK252_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOT13963.1"
                     /translation="MSKVLIIGAGGVGTVVAHKVAQVKDVFTDIMLASRTKSKCDAIA
                     KAIGDSRIKTAAVDAENVPQLIELINSFKPDMVINVALPYQDLTIMDACLATKTSYLD
                     TANYEPKEEAKFEYSWQWAYQEKFKQAGITAILGCGFDPGVTSVFTAYAAKHHFDEIQ
                     YLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYWENGKWVETQPHEIHKPLNYPGI
                     GPKESYLIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMARIDPVMYNG
                     VEIVPIQFLKAVLPNPGDLGENYKGWTSIGCRIRGLKDGKEKTYYVYNNCSHEEAYKE
                     TGTQGVSYTTGVPAMIGAMMYLKGLWKKPGVFNVEEFDPDPFMEALNKHGLPWVEVHN
                     GDLEL"
     gene            complement(28236..28457)
                     /locus_tag="PK252_04300"
     CDS             complement(28236..28457)
                     /locus_tag="PK252_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13964.1"
                     /translation="MASIILYQGMTKRIVIYPKDVMTITGRSEKFSRNLLKQIRQQLD
                     KEQHHFVTIYEFCQFTGMDAEQVQPLLVS"
     gene            28643..29392
                     /locus_tag="PK252_04305"
     CDS             28643..29392
                     /locus_tag="PK252_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13965.1"
                     /translation="MANQKGIIKIEGTVGGMTFYKKDGEYLVKEKSSISADKIAKDPA
                     FARTRENNAEFGAAGTAGKVLRDALRNMMLNASDTLVTSRVTQLMTDELKLDVTSVRG
                     KRLPANGLALATGKALLKGFNFNANSMLSGVLFKPYTVVTATGVITITGLNPATDIAA
                     PAGATHMSITGGMANVNFATGVWAASLTNVQNLALNAAAATVTLTPTAVPAGTGIKVH
                     LLKIEFFQLLNGVQYPLKNGSFNALAVVEVA"
     gene            29395..29655
                     /locus_tag="PK252_04310"
     CDS             29395..29655
                     /locus_tag="PK252_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13966.1"
                     /translation="MNEFQLFGIIISLLLSVVAFFVKQLHRDFKRVEKDLGEVKVTTS
                     LIKTEFKGINDLMNQKIEFLEKRLTHIETFIFKTIKKWQRLI"
     gene            29637..29855
                     /locus_tag="PK252_04315"
     CDS             29637..29855
                     /locus_tag="PK252_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13967.1"
                     /translation="MAKTNLTLVQRITAPTPKIFRIIRTVGLSLTAVGGAILAAPAMP
                     VILVTIAGYLSAAGAVMAAVSQVAVDGD"
     gene            complement(29931..30398)
                     /locus_tag="PK252_04320"
     CDS             complement(29931..30398)
                     /locus_tag="PK252_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13968.1"
                     /translation="MRSEASKIFIEETMHTFTVKSSTVKEESPKPHFFALNKGLNSGK
                     PLLQACPNCFKIEAENEELKETLYWVPFALWKAKAFHPYLIGSVIPFIRIGDYKKAIN
                     DKMEVVKVNQPEFIRAVEKLKFFDIKEKQMKEHLQLIQELKRAYALSCFNKHG"
     gene            complement(30565..31251)
                     /locus_tag="PK252_04325"
     CDS             complement(30565..31251)
                     /locus_tag="PK252_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13969.1"
                     /translation="MVFAKKSKNPASLPLPHVPSIGFVPQPSCPPPLLNSQWLRRVKP
                     SGAKKNCTLWLALLAHGCYNLLFTTHKNKKKRKISQYQCFNKYTKTKCKVTGLQAKAD
                     KGSLRQPQKIFLFLLPEKQKVKTKRVFFGLPLHLCLSACGIQALCLTVFFAVGWLAGG
                     WLPTLVVRFASTFLISVVPFRWFLFSSCGCNSLLVSCLFRWLTLFFCAFGQKKQGQSQ
                     KSKKCSKLGI"
     gene            31406..31537
                     /locus_tag="PK252_04330"
     CDS             31406..31537
                     /locus_tag="PK252_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13970.1"
                     /translation="MKCKDAESSEALKCNVAVMSEALLQDRVGCNERKQGNEKHDKL"
     gene            complement(31768..32352)
                     /locus_tag="PK252_04335"
     CDS             complement(31768..32352)
                     /locus_tag="PK252_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT13971.1"
                     /translation="MNRIETPEYPLPALREMLLNALVHRNYMGAHTQIRVYNDKISIW
                     NDGLLPDSITFEALKRQHASKPRNQIIADVCFKGGYIDSWGRGTIKIMEACKNANLPE
                     PEFKELSGGFLSELFKDRFSVENLQKLNLNERQTKAILFIRGKHSISNSEYIELCSVS
                     DRTALRDLDDLVEKGILQRIGEKKGSKYQLVFGG"
     gene            complement(32399..33091)
                     /locus_tag="PK252_04340"
     CDS             complement(32399..33091)
                     /locus_tag="PK252_04340"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT13972.1"
                     /translation="MPEQQNIEYKSNWHDDYLKWICGFANAQGGTIYIGMDDSGKVIG
                     LADYKSLMDEIPNKIRNNMGIMVEVNLLESDSLQYIEINTPPYSVPISLRGRYYYRSG
                     STKQELIGTALNEFLLKKSGKTWDDVIEPRATLADIDEQTIKLYLKASEKAGRLPDFE
                     NLSISELLDKLRLSDNNQLKRAAIVLFGKDPGKFYPSMFVKIGRFGNSETDLMFQEQE
                     EGNIIRLLDKCA"
     gene            complement(33266..33862)
                     /locus_tag="PK252_04345"
     CDS             complement(33266..33862)
                     /locus_tag="PK252_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13973.1"
                     /translation="MESITFDTVYSALKNKGYEIFETDTKPYNLNIIGIRNNNRVPNS
                     FDDYIVIMWKYKGQWNFHKYEATTDPGLYYLNHPLSPQGTAILKADQYFHCYCLGLHQ
                     GKYKALVQFMPVTVIRDFNKNNYLDFTSGKEETGIFGINIHRANLTGKSINVNQWSAG
                     CQVFADSGQFSEFISLCEMAQKYWGKTFSYTLINFNDL"
     gene            complement(33889..34497)
                     /locus_tag="PK252_04350"
     CDS             complement(33889..34497)
                     /locus_tag="PK252_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13974.1"
                     /translation="MTFDFTKGLTFIESTGKKLYHPSFKVIPEDYEIIYKLLVYILKD
                     NAGAEKHKLSLRKGILLSGPVGCGKTSLMNIIRHIQPLEERFVMKTCRDVSFEFIQEG
                     YSVIHKYSKQSFKNDIPRTYCFDDLGTENSLKYFGNDCNVMAEILLSRYELFVSRQMF
                     THLTTNLNSSEIEDIYGARIRSRLREQFNLIAFSESAKDKRV"
     gene            complement(34528..35349)
                     /locus_tag="PK252_04355"
     CDS             complement(34528..35349)
                     /locus_tag="PK252_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13975.1"
                     /translation="MNYIKHLTGFFDRVVPDDRLNPTHISLYLSLFQFWNINRFQNPI
                     SICREETMKVSKICSKATYHKCMKDLHNFGYVKYMPSYNPFKGSLVHLVIFDPDYKPV
                     QQTDGQPFKNQSGNETTTGTGTEQALVPSINNTNLLNIINDSDANSQTQFSKNENLPF
                     SENNNPGMLQSQNSKRKKVAPKKEKPDSIPPIMEEVIAFFNTEQFPIVEANKFYNHFE
                     SNGWRVGGKTPMKNWQAAARNWMLNAQHFIAPQGLPKPQSKPQPSPNGKNYNEPL"
     gene            complement(35346..35666)
                     /locus_tag="PK252_04360"
     CDS             complement(35346..35666)
                     /locus_tag="PK252_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT13976.1"
                     /translation="MPTSIITTDDLREFKTELLEDLKKILKEQGCQSEQKQWLKSAEV
                     RKLLNISPGTLQNLRVNGTLSFTRIGSIIFYNYNEIEKIINGNQINNQKPHPSNLQKG
                     KSGL"
     gene            complement(35832..36677)
                     /locus_tag="PK252_04365"
     CDS             complement(35832..36677)
                     /locus_tag="PK252_04365"
                     /inference="COORDINATES: protein motif:HMM:NF020914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HOT13977.1"
                     /translation="MSDHICNICQNLDDDLKTIESEFSNILKISERSIVCISACLKEL
                     KDFVSQNKFPNQEAEILFFKETKPAIYSKLIYFIKIFNIESKRPNGSDESQKRYLQNE
                     LNKLEKFFAENLEFYQYMRNKLVYLDDKYFVRGKYDLHLYLDTFYFDADPAFSTSHDF
                     KVAKILANDLLCVYLKTELALIDRKEFKRTDQVRKSKYAWTDPKIALVELIYALQACN
                     SINNGLIDIKEIALFVEAVFDIDLGDFYRTYLEIKQRQNPSKFLDALKTALLKKIEQD
                     DEHPR"
     gene            complement(36670..37308)
                     /locus_tag="PK252_04370"
     CDS             complement(36670..37308)
                     /locus_tag="PK252_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOT13978.1"
                     /translation="MRIGGFVKQSLIDYPGKIAAVVFTQGCNFRCGYCHNPQLVLPEL
                     FIEHLELAPINIISYLAKHKNWLDGVVVTGGEPTIHKDLPLFLKELKDLGYSIKLDTN
                     GSNPYMLEQIVENKLVDYIAMDIKIAPEKDLYGKIIGIQMTDEIIEKIMVSILLLNNT
                     SIEGEFRTTFIPGIHDEATVNLIQRLVGQNKSYKVNEFREGKTIEKSVVSYE"
     gene            complement(37460..37975)
                     /gene="cobO"
                     /locus_tag="PK252_04375"
     CDS             complement(37460..37975)
                     /gene="cobO"
                     /locus_tag="PK252_04375"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT13979.1"
                     /translation="MKGYIQIYTGNGKGKTTAAFGLALRAVGAGKKVYFAQFVKGQVY
                     SEVKAVFNYLPTISIKQFGLECFIVKSPTQADIDIARQGFKEVSGIILSGTYDVVILD
                     EANIALYYNLFSVDELIQLLKKKPEQTEIIITGRYAPQELIDMADLVTEMKEVKHYYT
                     QGVEARIGIEC"
     gene            complement(37972..39105)
                     /locus_tag="PK252_04380"
     CDS             complement(37972..39105)
                     /locus_tag="PK252_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13980.1"
                     /translation="MKQIILPILALLFITQSCKKDPIEPVVDPPVVKPVTDTTYTTKG
                     LYVLNEGLFNMNNSTLTYYSFADSVPQTDYFNLQNGRKLGDTGNDIAIYGNKMYIIVN
                     VSSQLEVVNPFTGKSIKQIPFFNGDKPRQPRYIAFNKNKAFVCSFDGTVAVIDTATLE
                     IEKYITVGRNPDGIAVANNKVYVSNSGGLDYPNYDNTVSVIDVATLVETKKITVRINP
                     YIIVPDKYGDLYVISRGNYGDVKMCLQIIDSKTDELKYTFPGFEALNLAINGDTAYVY
                     YYDFMTGSGSKIMLINVKNETIIRDNFITDGTVIETAYGITVDKQSGNVFITDAHGFV
                     NTGNVVCFSKEGKKKYSFKAGLNPGHIAFLNETKIDTLKTTIK"
     gene            complement(39117..41105)
                     /locus_tag="PK252_04385"
     CDS             complement(39117..41105)
                     /locus_tag="PK252_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT13981.1"
                     /translation="MKQLFLIILFSFSVFNLFSQKLIKDTVKLEEVTVTAVKPPEKDK
                     SVVPMQSVTRVELDRISGTSAADAVKTFSGITVKDYGGIGGLKTVMVRSLGANHTGVF
                     MDGVQFSDVATGQVDLGKISTDNADNVSLFIGQPTIICQPARYYASASVISINSSIPN
                     FSNKPFRFKVGCKIGSFGLINPLFSIQNRLGQKSYSDISCNYTKANGEYPFHIQLGRS
                     KDTTAIRKNSDIESVNLNAALVTILRDSSRLSLKMYYYGSERGLPGAVVFYNPFATQR
                     LWNKDFFSNLQYKSDTKKRLQWLTNIKYSQNWLRYLDPEYLNAEGKLDNRYLQREYYI
                     SQAVNWKVIDSLHLSIASDFFVNTLDANLYQYAKPTRYSSLSVLAFQYLRPRWEVNGN
                     ILATLVREKTMEGQPAPDRNILTPSVSCGYRLTQSPNIRLRFLYKDVFRMPTFNDLYY
                     TLVGNNNLKPEYAKQYNAGVTAFSHWGIIEYISFKADFFYNHVKDKIVAVPTKNLFVW
                     SMRNIGLVDIKGMELHGQLQTKPVLGLRYSLSCNYTYQAATDVTDENSATYNQQIPYI
                     PFETFTAMGTVAYKSFSVSYNTLFNGYRYVLGENIYENMIPSWWTSDISALYEFKIKD
                     YTLKMKGEVNNLLNKQYEVIRSFPMPGRSYTVALTLLF"
     gene            complement(41156..43159)
                     /locus_tag="PK252_04390"
     CDS             complement(41156..43159)
                     /locus_tag="PK252_04390"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOT13982.1"
                     /translation="MRKILLFMAGCMCIMHNSPAQNKYAPGAGNTGSTAIYTDSSVIK
                     SWATGIELYRGWVNIADTSVKYNGTNKASFGYPAIALGKAQNSPYDVVSLGDSGVAIL
                     SFDRPISNGKGPDFAVYENGFYTPAGQKSTTFSELAFVEVSSDGKRFVRFPAVSLTPQ
                     SPQLGGFAAIDPTNIYNLAGKDIAGYGTPFDLDDIKDSTGIDLNNIRFVKVVDVVGNI
                     NAPYASHDSKGHIVNDPWPTPFHSCGFDLDAVSVVNAGQPYSITTLDELPLAKDSWWS
                     PTNDTSWASGLAKFRFFHDTNFDYGFMYSNNRNDTAIDQSKLETSTLSAITKGGMNAP
                     DTGGTNYAVAFVAQNWLGDFSNMPVEITFKDDKSYAVSGFYVTNCTYAYLSMRDGDMF
                     AKKFGGTTGNDPDYFKLLIWGEKQDGSKTDTIEFYLADFRSNDNSRDYIVKNWRWVDL
                     NKLGKVKKLRFNLASSDVGSWGMNTPAYFCIDNLTIIPDAQPVKLTPLDDVNVNMNAQ
                     PLNVDLSSAFTAYDPSGITLAVASNSNTGLVSTNLSGKTLTLTFLANQHGESEIVVSA
                     TLNNQMVTDTFKVTVTNSATGIHNALANNVKVYPNPFSANLNVECEANSRVILLDIYG
                     RIIEEQITAGNTVQFSTEHLTPGYYILKISGKSDSQSVKILKQ"
     gene            complement(43173..44039)
                     /locus_tag="PK252_04395"
     CDS             complement(43173..44039)
                     /locus_tag="PK252_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT13983.1"
                     /translation="MKKKHLFLSLVLSIFLSINAFSQFSFNDIQLWAGSGTNKAALVI
                     EWTTPDGNYVTKVWGYKWTGTKTGRDMLNAIKAIDPRIFEKAGSYGDQTVYGLGYDMD
                     CDGFTYVAGANETGHAGDSDDVYKEGWLYQGYWSYWLSTNGTSWTYSNYGIANRTLTN
                     GAWDGWRFAYAPSWTANPPRTPFTAATACGSLKSASTSSVASENSALSLPKAVEYDIN
                     ISPNPCTEFINIYTDKPAQVTIIDLQGNEILKESYSTQSPSVNVANIEKGIYTLKIAT
                     DGKTVYKRIFKN"
     gene            complement(44058..44636)
                     /locus_tag="PK252_04400"
     CDS             complement(44058..44636)
                     /locus_tag="PK252_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13984.1"
                     /translation="MTEKKNLHLRYGVITLMVLLAAFSRLLPHPPNFAPIGAMALFGA
                     AYFSQRYLSFLIPIISMWLSDLVLNNVVYSQYFDHFVWFYQGCYWTYGAFIMIGLIGF
                     VALKRIKIRNLILASLLASIIFFLLSNFGVWASTNMYPKSFSGLMTCYAAGLPFFKNT
                     LLGDFVYIGVLFGAFEFAQSRMPVLRLESIKY"
     regulatory      complement(44750..44935)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(44942..47083)
                     /locus_tag="PK252_04405"
     CDS             complement(44942..47083)
                     /locus_tag="PK252_04405"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOT13985.1"
                     /translation="MVINGALKNVQISKRDGRILPFEAEKITFAIFKALRAVGRPDRQ
                     LAENLMLDVLKQLSMFEKVNFTPSVEEVQDTVEKVLFENKLFDVAKAYILHRKQHENI
                     RNAKELFSNLDIVEKYLSLDDWRIKESANSSYSLQGLNQHIATIVSSQYWLERIYSDE
                     IKQAYQSGRFHIHDLGFLSVYCVGWDLEDLLITGFKGVTGKIESRPAKHLRTILGQIV
                     NFFYTMQGEAAGAQAFSNFDTYLAPFIRFDKLEYAQVKQALQEFMFNINVPTRVGFQT
                     PFTNITLDLKIPEFLKNQPVVIGGKPQEATYGDFQPEMDIFNAAFAEVMAEGDANGSV
                     FSFPIPTYNITKDFEWDNPAYTKIWEISAKYGIPYFSNFVNSDMSPDDVRSMCCRLRL
                     DKRELQKRGGGLFGANPLTGSVGVVTINLPRLGYESKSETEFFQRLDKLMELAKESLE
                     VKRKVIENLTEKGLYPYSKFYLRHVKQRTGKFWKNHFSTIGIVGMNECCLNFLGCNLV
                     QEKGYAFAQKVMDVMRSKLETFQDETGNIYNLEATPAEGTSYRLAKIDKAEYPDIVTA
                     NEVHVRKGAKPYYTNSTQLPVNYTDDLFEALTIQDGLQTKYTGGTVFHTFLGEKQLHV
                     DSVKALIKKITHTFHLPYITLSPTFSICPEHGYLFGEHKQCPKCAETGQTSECEVFSR
                     IVGYLRPVGQWNDGKQEEFKERKLFDKMIKD"
     regulatory      complement(47202..47407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(47520..48764)
                     /locus_tag="PK252_04410"
     CDS             complement(47520..48764)
                     /locus_tag="PK252_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOT13986.1"
                     /translation="MNTTFSLLFYLKKPKTYETGPVPIYLRITVNSQRAEASTGRECI
                     PDKWIPSAGRASGNKEESKALNTYLDTIQTKVYEAHRQLLNSGNLITAETLKNKYIGK
                     GEKPRSLVKIFENHNKQIEALVGSEYASGTLQRYKTSLKHTIDFLKWKYSVSDIDIRQ
                     VDHAFITEFEFYLRSVRKCNNNSAVKYIKNFGKVIRICIANDWLEKNPFRNYKAKIKE
                     VERAYLNNEEIQAIANKRFKSERLNQVRDIFLFSCYTGLAYADVKKLDSSQISMGIDG
                     EKWIYTHRQKTDTRATIPLLPMALEILEKYKNHPQCLNSGKLLPVLSNQKMNSYLKEI
                     ADFCEIEKELTYHIARHTFATTITLTNGVSIESVSKMLGHKNLTITQHYAKILDRKVS
                     DDMMLLSEKLSSKPKSRLSKIN"
     gene            complement(49027..49114)
                     /locus_tag="PK252_04415"
     tRNA            complement(49027..49114)
                     /locus_tag="PK252_04415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49078..49080),aa:Ser,seq:gga)
     gene            49256..49873
                     /locus_tag="PK252_04420"
     CDS             49256..49873
                     /locus_tag="PK252_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOT13987.1"
                     /translation="MWKEHTKKFYRRIFDYFLQGLLYIAPLGLTVYFFVLILQFIGGF
                     AQEYFDKWLPFHIPGLGVILMFALITIAGYFGHKLIFTPFKLLLEKLLSKAPLIESVY
                     TSIRDLISAFVGKDKKFNQPVLVKVNKTAELEKIGFLTANDLSALKVKGKVAVYFPHS
                     YAFSGELFIVPSELVTPLDITPAEAMKFIVSGGVTEINDNEPDKK"
     gene            49870..52533
                     /locus_tag="PK252_04425"
     CDS             49870..52533
                     /locus_tag="PK252_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13988.1"
                     /translation="MSAKTKISVGIIVVAFFCLGILFWQYFKPQKILLSPLNAVPENT
                     AIVIKINGILPFLQHLQENDVYRAIKDERFKTEIAILSNKLDSIAHDISGGLSLFSDS
                     SVYIAIEGLYNNKPKILVLFNISEKITISEIKGYGQDVFGNTFQKIDTALISNVSALD
                     TSWGFYLKSGILVLGNNYHFLEASKKCLTGRHSLFSDTLFRQMANIAGKKVAANLFVQ
                     PKRCLKALKPYIAESIYNSLELMVADKGFTALDINIDNSFIAFQGFSKGTASNSLWPK
                     ILRAEEPVEAVDWRLLPEGVYNFSAFSMSDPYNYTISTNELYKAIGQWSENIRKLNDD
                     LNNNLLKSFQQCIGSQVISMTVKSPGGQLNNYYIVKTNQEDATVRFLNIICKEEKDKP
                     VKSDTSDVILIRKLKNPDIIKVLFDKKGDAGKYEIVCKYNDFLVFAESEYALYSYLDV
                     LKVNKSILNDSVFVSGCKTLFSTQSNYITYFNLSKASEVFFENLTNKGIDVASRYLGL
                     ETGKATWGFQLSGNNEMSYCNGFIRNEVDVNPVSMKLWEVPVSAGVAEVYKMYNADSS
                     ICRIIAQDSLRNVYILSGKGDVLSKISCHSKMLGQPVFYKNKSKQDCIAFNSSDELYF
                     VTFNGMLLENSPIKLRNNASAGIATADFDNNGNYRFYLPLENKDIVALDQKGKKVDGW
                     AFKKTNGVVRLPILCFSYKNKDYLVVADDNKPLFLMRNGLERFKVNKAAKWENIAVMG
                     VEKLGRYFKLYTQEKDGCLKSVFPDGHIEKLHCDSFSMFLNGQQIFFVKKDSLTIKLY
                     NTNFKPLIDRALFNTVSSITDYTFSDNMGFIAAFDKLSGLVTLCDQQKVVSGFPVNAF
                     SPFIFCTLKKHKPDNIIVVCENKKLAAYSVP"
     gene            52703..54397
                     /locus_tag="PK252_04430"
     CDS             52703..54397
                     /locus_tag="PK252_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13989.1"
                     /translation="MKNQMCIVPKILFAGFVALLAWGCVDNEYDITDISMKMEYDPNL
                     AFPIGSTTIKLGDLLEKYALPENLKEGTDGSFTVEYEEELEPLRGEQVFLVPLQNQIV
                     VPGVIFPATIPASGSVTREVTFIFPVRFLETQQVDSLITKCFYANIWGQSTFDPALSL
                     QAKIAFLDMKKDTVIGGSKTSLTYTEEFSPTANYNYLTPNKYKAYNYKLRFSSTKDSV
                     SETSVNVSLTLSGAAGTPINPASYFTAIMQLNELRYRVMYGYLGKKTILDMVYTFNVD
                     FLNREMARNINWYNPQIGIKIRNSYAVPVQFSIDKIEMFSSVQNQLINVSPDATANNP
                     RFINSPKAAFQEVSDNISFTKYNGYKSFYEGIENSAPKSIIYHVSAVANPDAVIDKHN
                     IAMDTSAIFPKFYFKLPIWFRSGGFGYTDTLDFDLAGMQKDNQDVELKSLLIRLVSIN
                     NMPIDMNLQAYFLDEHNNILDSMFNTAGQSRQVLKAGTVDPATSLVTAPTKTVKDISF
                     TPSQLSKMKNMKKLIYKVSVATALYDTQPNLYVKFQKQNSLKISFACQVQPKITRE"
     gene            54422..55840
                     /locus_tag="PK252_04435"
     CDS             54422..55840
                     /locus_tag="PK252_04435"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOT13990.1"
                     /translation="MRQAIIAIVILFTGINVKSQEPNTLYFMDRVPQASMMNPAFVPK
                     CNFYFGIPASNFHNNINFAPLKFNIIFPYDNVDKTVGNFMRNDNTKASFLNTLNSNNE
                     LYTDFQDDILSFGFRIKDWYFHFNASARSTFSFAYPKTFVESLLINGFELNTMYDYSK
                     LGLSMNSYVEFGFGLTRQINESWTVGAKMKVLRGIANVRTNNNQFSIGTVDENDLYVL
                     RVKSDFSVDMAIPYYKVNKGVNLSLDSLIEKDNDYKIKPFENIGFAWDFGATYSGIEN
                     LVLSASVIDLGSISWKDNISNLKLSSNFEFDGTDFVADSFDNAFDDSFEALKDSFKFT
                     SSSNVSYSTALPTKIYVGGEYSLARFFSIGVLSSTRIYQAEYSEQLTFSANLKPAKMA
                     MLSLSYSIIDNAAHTFGMGFSFRIIPPTQLYFVWDNIPVYYGKNFFPYKMDRMNIRLG
                     MNWVFGCNGRTKKLKDKPMGWE"
     gene            complement(55844..>56242)
                     /locus_tag="PK252_04440"
     CDS             complement(55844..>56242)
                     /locus_tag="PK252_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT13991.1"
                     /translation="NIPEDENAIYINGNVRLLKVMFTNLIDNACKFSSNHKALVKIGH
                     ENNLAIVKVIDKGIGIPANDIELIFKPLYRSKNAGDLQGSGIGLSIVRKVATIHNAQI
                     EFESEQNIETTVTVKLHNCLDLSIDAVSIS"
BASE COUNT        16197 a        10378 c        11341 g        18326 t
ORIGIN      
        1 ttagcaaatt agcaaattag caaattagca aattagcaaa ttagcaaatt atatttctta
       61 gacattctcg aattttcaaa ttttcaaatt ttcaaatttt caaattttct cattagtatt
      121 cgaactttcc gtttaccgtt tccagcgtaa gttttttgcc ttggtgtgct tcgcatcggc
      181 ctataatctt ggcttcgatg ttgaattttt ctgaaatgct tattatttct ttagctgtgg
      241 ctgcatcggt gtaaaactcc aagcggtgcc ccatgttgaa gactttgtac atttctttcc
      301 agtctgtttt gctttgttgt tggatgattt caaacagcgg aggtgttggg aaaaggttat
      361 cttttattac atgcaggtta tctacaaaat gcatcacttt ggtttgtgct ccgcctgaac
      421 agtgcaccat gccatgtatt gcaggcctga gcttgtcaaa tacagcctta acaacaggcg
      481 cgtaagtacg ggtaggagag agtacaagtt tgcctacatt taccccttca acaggcgaag
      541 ggtctgtaag cttatatttt cctgaataaa ccaagtcatt aggtacggca gcatcaaaag
      601 tttcggggta aagcttagcc aggtactttt caaatacgtc atgacgggca gaagtaagcc
      661 cgttacttcc cataccgccg ttatatgagg tttcataaga tgcaacgcct gtagaagaaa
      721 ggccaacaat tacatctccg gctttgatat tggcattgtc aataacatca gagcgtttca
      781 tgcggcaggt aacagtactg tcaacaatga tggtgcgtac aaggtcgcca acatcagcag
      841 tttctccacc agtaggatac acgccaatac caacattgcg cagttcagca caaagctcct
      901 cggtaccctg aataatggcc gatattactt cgcccggaat gagtagtttg ttgcgtccta
      961 tggtagatga aacaaggata ttgtcagtag ctccaacaca cagcaagtcg tcaatgttca
     1021 taatcagggc atcctgagct atgcctttcc atacagacaa gtcaccagtt tctctccagt
     1081 aggcataagc tagtgatgac ttggtaccag caccatcggc atgcatgatg ttgcaataag
     1141 cagggtctcc accgataatg tctggtatta ttttgcaaaa ggcttttgga taaaggcctt
     1201 tgtcaatatt tttaatggcc tggtgcactt cttcttttcc ggaggaaaca cctcgcattt
     1261 tgtatcttga gttatcttcc atttgtgcca taatttctgc tatttaaacc ggataatctc
     1321 tgccgggctg aaacgtgaaa taattgatga tggtatgatt aaaaataaca atgttgctat
     1381 ggctacgcca acgttaagca atacaatgta ccatgggtca agcaaaatag ggacatggtc
     1441 aatgtaatac gagctggcat cgagttttaa gaaacgggta tattgttgtc ccaaacaaat
     1501 agcaagtcct acgaggtttc cccagaacaa tcctttgaaa aggataaaca cagattcgaa
     1561 aagaaatatg cgccttattt ttttattagt agaaccaagg gctttgagca atccaatgag
     1621 ctcggtacgg tcgaggataa ggataagcaa cccggctatc atgttaaagc cgcatactac
     1681 aagcatcagg atgatgataa tcatcacgtt gaggtcttgc aggttgaccc agtcaaatat
     1741 ttgcgggtac ttttgtttaa tgctcagtac ttgtaaggcc tgaccgtttt ccataaagct
     1801 gtagcccaca atgtcattga caacaaccgt cattttgtcg atgtcgtcaa atttgtttac
     1861 aaaaacctca aagcctgaaa tttggtcgtc tgtccagtta ttcaacttct gtatatgctt
     1921 gatatcgcag agtacaatta tcttgtcaaa ttcttctacg ctggtttcgt atattccgga
     1981 gatagtaaac acacgtaccc ttggtgggtc ttggataaaa tacatgctta tttcgtctcc
     2041 tactttcagc ttaagcagtt tggcaatata tcttgaaatg attatttcat ttgttttttc
     2101 gctgtcttta acggtaaaag tcttgcctac taccatattt tgcctgaaaa agtcccaact
     2161 gaagtcaggc gcaatgcctt ttaatacaac gccctggatg tctgtttcgg ttttcaggat
     2221 tccggctttt gtggcaaatt gttgaacatg ctttatgcct tcagcatttt tcaggtcagc
     2281 gataaattct tgtttgttgc ttatgggagt agtttcgtaa gagctgttgc tatcgtagtt
     2341 gaggatttgg atatgcgagc caaaaccgac caccttgttg cgtatttcgt ttttgaagcc
     2401 ttgcaggatg gcaatagaaa taatcatcac agcaatgcaa agcgctacgc caaatatcgt
     2461 gacattgaca agcgaaccgg aaatttttcg tttcttgtca ttgcctttca gttgtcgttt
     2521 tgctatgaaa tattcgaaac tcaaatgcgt ttttgtataa gttgcgcaaa ggtagcaaaa
     2581 ccaatggtct attttaatat ttttgttcct tcaattttta aaagcatgat ttttagaata
     2641 ttattcgcct gggtcttgtt ttgttttttt cagttgttgc caagtcaggt catagagccg
     2701 ggtgcttggc agatttctga ttatttgccc atgttgaaag aaaaaaaagt tggtttggtg
     2761 gccaatcata cctcagtagt gaaggatgcc catttattag atactttgtt gtccttgaaa
     2821 atagatgttt ccagggtatt tgcccccgaa catggcttca ggggtgagac tgaggctggt
     2881 gagagagtga aaaatgaagt ggatgccaag accgggataa aggttgtaag cctgtatggc
     2941 aggcattata agcctactaa acaagatatg gataagctgg atattgtggt gtttgatatt
     3001 caggatgtag gcgttcgctg ttatacatat ttgtccacct tgcattatgt aatggaagcg
     3061 tgtgcccgat ataacaagca attgatagtg cttgaccgtc ctaatcccaa tagtttttat
     3121 gttgacgggc cagtgttaga gctaaaatat aagtcatttg ttgggttgca tccggtacct
     3181 ctcgtatatg gtatgaccat aggcgagtat gctatgatga ttaacggtga aggctggttg
     3241 ccagaagctt tgaaatgtca gcttactgtt gttccctgta aaaactatac acatgctgat
     3301 aagtatattt tgccaattaa gccatcaccc aatctgccta atatgcaggc gatatatctt
     3361 tacccatcgt tggtgctgtt tgaaggaacc tgctttaatg ttggccgtgg aactgatttc
     3421 ccttttcaaa catacggaca ccctcagttt gaattgaaaa acttttcata caccccgcgt
     3481 tgcaagaaag ggacaatgac tctgcattgt gataccttat gttatgggga agatttgaga
     3541 aatgtttcct gtaagggctt tactctcgac tatgtaataa atgcatataa tcgttaccct
     3601 tataaacagg aatttttcaa tcgttttttt gccaatctta ctggaaatac gtgggttaaa
     3661 gaacaggttg ttaatggaaa agaagtaagc gaaattcaaa aaatgtggaa aaaagatctc
     3721 gtttttttta aagattctgt cagggttaaa tatttgatat acaagtaaaa aagctgattt
     3781 ttgccatttt tttcatcctc gaaaaagaat tgttagtcaa aattaacttt atcggcatga
     3841 tttttgaaat acttttgcgc tttcaaaaat ttaaatgttt tgataagaaa aaaactttac
     3901 tttttagtac tggtgccctt gttgttcagc acctgtaact tcaaccgaaa caacctggct
     3961 acagaagccg atcttcccga gattcaaaaa cgggggcgaa tagtagcact caccaactac
     4021 aatacctcag attactttgt ttacaatggc aacccaatgg gttttcagtt tgagttgttg
     4081 aggcagtttg ctgagtattc gggttaccag ttagatattg ttgttagcaa caatatggca
     4141 gatgctatgc agatgcttat tgacgggaag tgcgatatta ttgcccaaaa cattgcccct
     4201 acgcctgaac atttgttgta tgcctcgttt acgtttccta tattgcaggg aaagcaggtg
     4261 cttgtgcaac aaaacaaaag tgagtcgccg gggcgctata tttcagacct gaaccagctg
     4321 aaaaacaaaa cagttattgt atcatctgga actgcctttg ctcaaagatt aaaaaacctt
     4381 gaaacagaga tagccggtaa aatagatgtc gtagaagtgc ctgaagatga agaacagctt
     4441 attaggtttg tcgcaggtgg cgaaattgac tataccgtgt gcgatgaata tactgcaagg
     4501 ctcaataaac aatatttccc aaagcttgat attaatgtag aggtgagtat tcctcaaaat
     4561 atgtcgtggg ctgtgcgcaa taactcgccc aaattattgg aattgctaaa ccgctttatt
     4621 gaagcagaaa agaagacttc ccgtattgct gtgttatata ataagtatta tcaaaaccag
     4681 tgggcgcaag ggatggtcaa tagcaagtat tttgtattga actccggaca actttcgcct
     4741 tacgacgagc tgctgatgag ttgtagcgct gagcttcaat gggattggcg attgctggct
     4801 gccattattt ataaggagtc gcagtttatt cccaatataa aatcccacag gggggcggtt
     4861 gggctaatgc aattaatgcc ctctactgca ggcttgatgg gttatgattc tactcaggtt
     4921 gaagaccctt caaaaaatat tattataggg gttaaatatc ttaagtggct tgacaataag
     4981 atatcttcat taatcccagt caaagaagaa cgtattaagt ttgtattagc tgcatataat
     5041 attgggttgg gccatatttt tgatgctatt gaactggcta aaaagtataa aaaggatgca
     5101 accaaatggg atacagtgaa agattttttg ttaaacaagg ctatgcctga gtattaccgt
     5161 gatgccgtag tgaaatttgg cccttgtgtt ggaaagcaga ctgttactta tgtatctgaa
     5221 gtattaagct gttattcaca ttacaaaaac cttacaccac agtaaatatg ctacttccca
     5281 ttcttgcttt ttaattcttc cagtctttgc tttttcttta gcgtgttgct ataagcacta
     5341 caggctgcag aacttgaacc ttttgatttt ccggacgagc aaccaaacaa gaggttgaag
     5401 ccaaagcgga ggttgacaaa ctttgtagat ttccatttta tcataccata aacattgtct
     5461 gcaatggcgt aaaagccaaa tgtaggggtg cgggcgctga taccagcccc gagattcatc
     5521 aaagtgccgc gcgtaaacgt ccagctcagg ctcccggcca gaaagttttt gtattttgca
     5581 ttaatggctg ggcttattcc ttgcaatatt ttcccatgat aatattcgga gcggtatgtg
     5641 aggctggcat ttgcccaatc attcaaatca taagaagctc cgaggtataa cttgggtgta
     5701 aggaaggtaa aataagatga ttgagaagtt gaatagctat aagcatcttg tgcagcattg
     5761 actactgaat cgaggttgtt gaatgttcct gttaaagggt cgtattgaat gccattgaaa
     5821 ttataactac cactaatgtc aacttgtgca ggtgaataac gccagtaaat acccccaata
     5881 tcaagcaaac tggctgctac agttgtgtta tcgtccagtt ggtaaagcgt gccaaagtca
     5941 aacgagatgc ccgggttttg ccggttgaga agcaaagata caatgcttgc atctacaagt
     6001 tctatggacg ttggctgatt ttgggcatcg gtagtaatta caaacggaga tgctttaata
     6061 agataatgcc ctgaagcgct tagttgcaaa gagtctgatg tagtggtcag cgctagatcc
     6121 ttttttcgtg tttgaatgtt cactttgcca aacagcaact tggcatgaat gcccaaagtc
     6181 cattggttgt caattttttt tgaagcgcca atgccccatt cacgataata tatgccatgt
     6241 aaggctgtcc ccccaacatc aaatgtttcg cctatatgcg gtgcattgcc ttgcagggta
     6301 aggcttatga ggttaccgga atagttcaac cctacgtcta ttttatcctg aaggtgaaag
     6361 ttgaagtaga tgtctttgta aaaaaatcca aagtggataa ggcttatttg cgtttcaagg
     6421 cgtatgtttt ccaaatctcc tgaggcattg ataaggtaat caggtttgaa aacactgttc
     6481 ccggactggt tggtagtgat gatatctttg tatctaaata aggtattgga atatccaaca
     6541 tggattgacg acaaagccgg gataccaaca tagactttac aagggatttg tactgccggg
     6601 tttagtatat tggcttgtgg taaggtgtgc ataaagtaaa gcgtatagtt gtcctgtgct
     6661 ttcgcctgaa atacggttaa aagcatgaac ataataaaga tgctatgttt agcctttatt
     6721 gttatcattc gcttatcagg tagtcaaatt ctatgattac agcgatatac aaattcacct
     6781 tgtatgtttc ataatacttg tattgctcgt tcttttttat ctggttgttg actgcacagt
     6841 acagatgaat gtattgtgct ttaagccatg agtctatctg ttgtttgtcg agggttatgt
     6901 ctgttctgtt tttagcttct tccttaatgg taaggtcttc attgtattgg gctgcatcaa
     6961 tgtctatttt tgataagaag cttgaaccca gggtgctttg attgtttgat gtgtcaaggg
     7021 ttacaaatag tgccgcattg gccgggaatt ggttagagct gaaaatacgc agggtcagtg
     7081 attttatata tttgttgttt tggtcgaatt taaaaatgtc aatgggtacg ttgtctccca
     7141 tatattatgg tgtctggtat ttgcaggtat tcggggtgtt ctttccatgc ctcaggcata
     7201 tttatcccca taatctttag gtttaaatac gtattgccaa gtggaaaagc ccatgttccc
     7261 tgatattggt gctcagtttt gaggccagag aggtcatcag gttcatcaca tgctgcaaat
     7321 gcaaacatga taaaagcaat aaaaaatagg ggtatttttt tcatggcgtt gcttttgttt
     7381 ttataaatat acgaataaag agcatattga taaacgctca aagaggtgaa atagttcgtg
     7441 agatggtttt tgtagaaaaa taacttgttg agagttttat agttatttag ttgctatata
     7501 tatgtagaac tgggtaaaca tatcataaat aaagctatgt cattgatata tcagtgctac
     7561 atgcctcttt tgattatatc tgaacaagta atttgttttt gttatttgag ggataacaat
     7621 aaatgatgtg tctcatggta aacttagaaa ttaaaattta ttatgatgaa acacatttat
     7681 tccttaatcg caggttttat cctgtttagt gccaatttgt attcttgcac agtcttttcg
     7741 gtcagtaatg ctgatacagc tttgtttggt aatagtgaag cctggttgcc gggttcaaaa
     7801 acccgttatt gggttattcc ggccagtgac aatagtttcg gcagaatttg tttcggttat
     7861 caaggcgaag ctttggccca aggaggcatg aacgaagcag gcctggcgtt tgattgggtt
     7921 gccagtcttg aatcttcggg ttatgaactg aatgacactc ttattacgct tgaaaccagt
     7981 ttgtccgaaa aaatactggc tgaggcttct acagttgatg aggccattaa gtattatgaa
     8041 aaatataacg aaccttactt ttctaatgct caaatactgc ttgctgataa aaatggtgat
     8101 gcagcaatag ttggatggca aaatggtaaa cttaaggttg aaaagaagaa tggcaaggct
     8161 attgtcttag gatatgaaga tacagtagct tttaattttc tcaaaaatat tgagccaatt
     8221 aatcctcaaa attgtgccac aatcctggct aaagtacttc aaagaggtat cattgctaca
     8281 tgttattcaa atatattcaa tttaaagaca ggagaaataa atgtgtataa ttttctggaa
     8341 acgagcaatt ctataaggct caacctcaaa gaagaacttg caaaaggtgc acattctgtc
     8401 aatctcaatg aggcattgtc ttcagtgaaa ataaaagttg cagaggctaa tgatactaag
     8461 aacaaggttt atcctactat atgtaaggat agttttacac tggaaatgag ggatgtcaat
     8521 ggtaaaattc tcgatgtttt tattattgat atgtttggcc gaaaacaaat gcatttcatt
     8581 gagaatgtgg ttggcagtaa tgtttttact aaaacatttc atattagcaa tctcaaagaa
     8641 ggcaattatt tagttattgt ctatgatgga atgaatcaaa gccagtataa ggtagtgaag
     8701 tattaatcat ttttttatta agaaaactgt atcgcaaaac ttctttttgt aaaagtcaaa
     8761 gtcgtctttt acatttgttg gaaataattt ttatcagata aagcccttct ttgttatttc
     8821 tctggttatg attgaagttt ctataaaaaa tatgcttggc gattatcaat ataatcctgc
     8881 tgatattgag ataattgctt caaaatttta cccggaaaca attaaaaaag gagctgtttt
     8941 ttgcgaaaga ggcaaacgta ttaacaaggt aggcatttta agcaaagggc tgttgttggc
     9001 ttattatgag aattatcagg atgaaataga ggtttcgcga tactttgata cagtagtaaa
     9061 tggcattgtt gtggactttg atgcgtttgt taatcaaaag ccatgcagcg aaaccattaa
     9121 agcaattgaa gactcggaga tgctttgtat taccaggcaa aatttatatc agctctataa
     9181 agaagtccct gcaatgaaca ttataggaag aagattggct gaagaaggtt atgtgttggc
     9241 cgtcagacgt attcaagcat tgcaaacgct tgatacagag gagcgtttcc ggaggttgta
     9301 tttggcaaat ccaacgctgt tcaatagaat ctccaaaaaa gatatggcca cctttctgaa
     9361 aatgaacaga aacctcatta ctcaatatct taaaaaagtt taaagcccta tttggcaatc
     9421 taaattgcat tttgttgttt ttcttgccca tatttttgtc agtaaaaacc taatagcatg
     9481 aaaaacacat tttacattat gtgcatctgt gtatgcacca ctttgttggc tcagtgccaa
     9541 agtattccaa ctactaacat catggaagat gaagtgtaca taaaatgctt cccgacaggg
     9601 atttacaact ccaattgtta tgttgtctct aaagacggcc ttgggtttat ggtcgatgcc
     9661 ggagcccctg atactactgt tcttcagtat ataagggaaa acaatattaa gataaagtat
     9721 atcttttgca cacatagcca tattgaccat gtgctttttg ctcctgaggc aaaacaatac
     9781 actggcggta aaatagtgct tcatgccaat gatgaagacc attacaagta ttacacctct
     9841 gaacgtattg gtgaatggat gaaaagcgga caagtgacag aacagcaaat gccgtatatt
     9901 gagaaattta tccatatcaa atatgacaca ttggttcatg gcgatgaaga atttgcaata
     9961 gggaagctga aagttaaaat tctttatact ccagggcatt caaaaggctc gatgtgtatt
    10021 tttctgaatg aaaaatatct ttttaccgga gatgctgtat ttaacgacaa tgtgggcaat
    10081 gctgagatta gttcgggcaa tatcagtgat ttggaaaagt atataaaaga agaaatactg
    10141 gttttaagtg acgatgtcgt attatttcaa ggccatggcg gaaaaatgtt gctaaaagat
    10201 ttattgccaa agatttcttt ttagcttttg gaaaaaagag ggtgtgtaaa aaggtcaaat
    10261 ttgttcattt ttgactctgc tcaaaatgac aaattgctga ttatcaatcg tcaggctgag
    10321 cgaagtcgaa gccttttcag actttttaca caccctcttt cagttttttg cttttctttt
    10381 ggcattcgta attcgtcatt agtaatcagt aattctcttt atctttgccg aaaaattaaa
    10441 gaatggcaat agattttaat aaagttcctt ccccttgtta tgtgcttgat gaagcgttgc
    10501 ttcgccggaa ccttcaaata ataagcaatg tacgagataa ggcgggtgtt gaaataattg
    10561 ttgcgctcaa gggttttgct atgtggagcg ctttccctat acttcgcgag tatgggttca
    10621 aacatgctac ggcaagctca ctcaatgaag ctactttggc caatgagatt atggggacac
    10681 cagctcatac ctattgcccg gcatatagcc ctgatgagtt tgatgaaata gttggtttga
    10741 gcagccatat tacgttcaat tcgctttcgc agtttgagtt ttttaaaaat aaaattaagt
    10801 cagccaacga aaatgtaagc gttggtttac gcatcaaccc gggttattcg gaagtaaaga
    10861 ctatgctcta caatccttgt gcaccgggtg ccaggttggg catacctgct caaactattg
    10921 gcaattcttt gcctaaaggt attgacggcc tgcactttca tactttgtgc gaatcttcat
    10981 cgtatgattt agagaagacc ttgactgtgg tagaagaaag gtttggacat ctttttggcc
    11041 agataaaatg gataaatatg ggcggaggcc acctgatgac ccgcaaagac tatgatacag
    11101 aacatctgat aaagctgtta caaactttta agcaacgata cccgcatttg catgttatac
    11161 ttgaaccggg ggctgtattt gcatgggaaa caggctatct tgtttctaca gtgcttgatg
    11221 tggttgaaaa tgatggtata aaaacagcca tgctcgatgt ttctttttcg gcacacatgc
    11281 cagattgttt ggaaatgcca tacaaaccgg ttattttagg ggctactgat gaagaagaag
    11341 gaaagcctac ctatcgtatg ggcggtaact catgcctttc aggtgattat attggtagtt
    11401 ggtcgtttaa caatccgcta aaggttggcg ataagatcgt tttcaacgat atgatacact
    11461 ataccatggt aaaaacaact actttcaacg gtgtccgtca tccttccatt ggtatttgga
    11521 acgacaaaca aggctttcgc cttgttcgcc gctttggata tgaggattat aaaaataagc
    11581 tttcgtaggg ttcaggaaga tagaataaag aataaagaac aaagaataaa gaagatagaa
    11641 caaagaagat agatgttgcg tttccttgtc tttgttcctt gttccttgtt cttttctttt
    11701 taatacctat acgtcaaata tttttcatat accttattcc cgtattcttt tgcagtgaca
    11761 tgcagcacta cagagtcttt ctcaggatgt tcaatagttg tgaggtcaaa cgatacaaag
    11821 ccatttaaag tatattgcgg ggcatcattt ttgtcattat gcctgatttc caattggatg
    11881 gtgtcagcgc caaatatccc cgcatttctg gtcaggttta tcatgtgctt taagttatag
    11941 ccataaaaac gaaaatctac gttcaggtag tttttggata tccatgtccc atttacaaaa
    12001 atcgggtcat tgccgataga gtctgctttt gtcgagtcat aaactattac aggttttaca
    12061 agcacctttt cgatgttata aacgtttatt acataatcaa ttcccttagg caatggttgc
    12121 tctgccatgg tgaaataagc tataaccctg tcgttgtctt tgaatgatga gcttaagctg
    12181 tttttgacca tcatcctgtt ccccttgtca gactctataa taacactgtc agcagttatc
    12241 tttaggttcc ctaatgtaga ataataggtt tcgttgtctg tttcttcgca agcactaagc
    12301 aacccaacca ttgaaataac aacaaacaat aatctatgcg tcttcatatc tttcgttttt
    12361 taatgtttaa ataaatgata tctggtaact gaaaaggttg cgttatcttt taatgaataa
    12421 tttaaccaat ttactgcaag ccttgaattt ttttactttt gcttcaagtc aaaagttagc
    12481 actgcctgta aatacaaaat acatgagctt ttttcttata tctgacgtta aatttagtgg
    12541 tttagtcctt ttggttgtat tactttataa atgtttttat cttcaaaatg ttttaaggga
    12601 aatgttaaaa aaacttgtct taaagtaaag tttgctctta agaaatatat ttacctttaa
    12661 ttgttaaaag gcacttcatg aaatatacaa taggcctggt tctcatttgt ttagccagta
    12721 ttgtccaact atatgccgat actattcctc agaggggtat tggctttgcg gcagggtatc
    12781 tgcgttatag ttctattgat tgcattgtta atccatacat ttattctggg caaaatatgt
    12841 cttattcttt ttcaacgttt tatagaaatc aaaagcattt tagacaattt gattttacat
    12901 tttcattttt gaataaagaa atagctcatg ttaatattcc agacagttat tcaattgtag
    12961 gctatgataa taataatacc tatagtgttg ccagcaatga atggcttaaa aaggtacctg
    13021 tccgtttttt tgttttacaa tacagctata gtcgaaggat gggcttcttc aattcttttc
    13081 aaatgggtga tttttattgg tctttgagta atatcaacca gacaattttt aaaaatgact
    13141 tgtcttatat ggagctaatt gcttttgggc tttatccagg cctgaaatac aggcataagc
    13201 ttttcgaaaa atattatgtt tcgtgcgatg taaatatcct tttggcaagt atcactatac
    13261 gcaggcctta tgccggtgtt gatgctcagc tttctgatgt gcaaaatgtt caaacattct
    13321 ggagttcggt gaaggcaaac aattatttca atttgcccaa taatcacctt gacctcaggg
    13381 cgcagatatg tcttgaaaga gctattggca gaaggtttag catgttttgt aaatatctgt
    13441 ttgagtattc tgaggttaag cggcctcgtg tcttgcaaaa ttcttatggc gcttttaatt
    13501 tgggtattta ttacaatttt tacagaaaaa tcaatgaata aaggtttttt agcaatattg
    13561 atattgtttg tttttacttc gtgtaacgaa agtttattga atgaggatgt tgaaaatacg
    13621 cctttgggaa attttgatgc tttgtgggat gcttatgata agacttacgg agcctttgag
    13681 gctaaaaata tcaactggga ctcgcttaaa attgtttatc gaagcaaact tagccaaaat
    13741 tctacaaata cagaattgta caatgtgtta tccggcttaa tccaatgcct taacgatggc
    13801 catgttcagc ttaccgcttc tggttataaa aggttttatt catccggggc ttataccatt
    13861 ttccttgatg ttaagtatta cgaaaatggc aacatgatta gcgtttttta taagttagat
    13921 tttagtaagt atataaacac cttgctggct gacgataatt ttgtatatgg ttatatgatg
    13981 gccaaaatca agtccaaaaa agtggggtac cttttggtgc ctactttttc atacaacaaa
    14041 tatcgtcaag atattgtaga taaggctttt gatgagttta aaagcaatgc ttgtgctata
    14101 attattgatc ttaggttcaa tggtggtggg gctaccgaaa cgttccttaa gcttttaaat
    14161 atgtttgctg accaaaagcg cttatacctt aagtctaaac tgcgaaatgg ccctaaacat
    14221 tctgatttta ctcaattata tgatcattac ctggaaccaa agtatggaac atttgatcct
    14281 aagcctgtta tcgtacttgt caatcgtttt tctgcaagct cttcagaaca ttttatgctg
    14341 ggaatgcaga ctcttcctta tgtcactgtg gtaggagata ctacttatgg cgctctttct
    14401 actgtgcttg aaaatgtctt gcccaatggc tggcaatttc ggacttgccc gcaagtgctg
    14461 tgcgatacct caggcaatta cttgcgtgac tcaaagggtc gctatcctga cggagtaggg
    14521 ctttgccccg atgtatatca gcccaattat tattcggata tcctcaatgg gcatgattat
    14581 atgctggaaa gggccatgca gattatatca gagcaggtaa aataaaaaat gcaaaacttt
    14641 tcaaaaagct ttgcatttca tcttaaattc tagttttcag caactgtaag caggttttct
    14701 acagctttct cgttttctac gccttgtttt acaagttcga tgactttatt taccatgata
    14761 tcaattatct tgtgcgagaa acctaatttt tgggctacat tcttgcaata taaaacttct
    14821 ccttcgtcaa tgtcgccatt gaccatcatt acaagtacca tatcgtacaa aagggcgata
    14881 cgctcttcgt ttgtttctgg tgccgtaaac ttaacgctgc cgggacgctc aataacacgt
    14941 ttcaattcat cataactcac gcccatgcgc tttcctatct ggaggagaag gtcaatttca
    15001 gcttgttcaa gcactccgtc tgcacatgcc acagccaaca ggttgaacat gtggctcaac
    15061 cttttctttt tttctctact gtcaaaaatg tcaaataatc ccataattat aaattgttta
    15121 agaatcttaa tttctaaagt attttattgc caacccattt gccattacgt agttgcatat
    15181 actcagtata tccggcatct tttagaaggt taaaagccaa gtcaaaccct tttactatct
    15241 cttcagcttt atgacaatcg gaattgatag taaccggaat atccatacgt tgcatagcgt
    15301 gcaaaagctc cgaagatgga taagataatt cagcagaaaa tttgtacatg ccacgggtgt
    15361 tgagttctac aatacaacct ttttctttgg ctgcaactaa gacttgtttc caggcttttt
    15421 tgtattcttc cgagctttca ttgaaaaagc ggttgttggt gttgaacatc ctgattttat
    15481 cgatgtgtcc gatgatggca ggctgcattt gctctatcat cttaatcacc gcttcataat
    15541 acttctgata aagcaatacc gggtcgttgt taaacagttc aacaatgcct tgttcgtatt
    15601 tttctaatac gttgtcgatg ttaaaagcct cgccatcagc aaagaatccc atgtaatgca
    15661 ctgaacctat agtgtaatca agcttttcga cttgcgatag tgctaatatt tgcttattgt
    15721 atggctcaaa gtaatccaac tccaatccac aatatatctc cagttgagat gcgtatttga
    15781 cttttagctc tttagcttta ttaatgtaat tggtaatgtc agaagctttc atgttccagt
    15841 ctgagtagaa tggaacaggg ctatgctctg aaaagcccat agaaataagg ttgcagttga
    15901 tagcttcgct aacaatgtct tccatcagac agtgcccgtc tgaaagactg gtatggctat
    15961 ggtagtttga tagctgcatt gttgggtatt ttaaaccttc ccggtttcag ggccctggaa
    16021 ggtattttat aagattttct tgtattttaa tttttaccct tcctgcctcc ccttgtaaag
    16081 agaagggttc gcttttttta acttaagcga caggtttagg gtttcaaata aaactctgcg
    16141 tttttgtcct tcagtgttag aattattcgt ggtgagtctc cagcaatatc tccataatct
    16201 tacggccagc ctttgataca gatgttcccg ggccaaagat ggctacaaca cctgatttat
    16261 aaaggtagtc gtaatcttgt gcaggaataa caccacctgc aataacaagg atgtcttcac
    16321 ggccaagttt cttaagctcc tcaataacct gtggaactaa agtcttatgt cccgcggcca
    16381 atgaagaaac gcccatcacg tgaacgtcgt tttcgacagc ttgtttagca gcttctgctg
    16441 gtgtttgaaa caacgggccc atgtccacgt cgaaacctaa gtcggcataa cccgttgcaa
    16501 caacttttgc accacggtcg tgtccgtctt ggcccatttt ggcaatcata atacgaggtt
    16561 gacggccaac catttgtgca aacttgtcgc acatttcgcg ggcagctttg aagtcctcgt
    16621 catcctggct ttcggcgcta tacacgccag atattgttct aatcacggct ttgtacctcc
    16681 ctgatatttt ttctatagca tctgaaattt cacctaaggt agcgcgtttt tgtgcagcat
    16741 ccactgaaag cgccaataaa ttgcctttgc cggttctggc agcttcggta atggcttcaa
    16801 gagcggcttt cacttctgca ttgttgcggt tttgtttaac ttgttgcagt cgttttatct
    16861 gggcttcgcg aactgcagcg ttgtcaacat ctaagatgtc tattgggtct tctttttcca
    16921 aacgatattt attaacgcca acaatggttt ctttgccact gtcgatacgg ccttgtttac
    16981 gagctgctgc ttcttcgata cgcattttgg ggatgcccga ctctacggct tttgacatac
    17041 cgcccaaaga ctcaacttct tcgataagct tccatgcttt atgcgccagt tcgtgggtta
    17101 agctttcaat ataatatgaa cctgcccatg gatcaactac tttgcagata ccggtttctt
    17161 cttgcaagta tatttgtgtg ttacgtgcaa tacgtgctga gaaatcggtt ggtaatgcaa
    17221 tagcttcatc taacgcattg gtatgaagcg actgtgtatg ccccaatgca gcagccatag
    17281 cttcaataca tgtacgggca acgttgttga atgggtcttg ctcggtcaaa ctccagcctg
    17341 aagtctgtga gtgtgtacgc aatgccagtg attttgggtt ttgcgggtcg aattgcttaa
    17401 caatcttggc ccacaaaaga cgtgcagcac gcattttggc tatttccata aaatggttca
    17461 tcccaatagc ccagaagaat gaaaggcgag gggcgaagtc gtcaatcttc aaccctgctt
    17521 taatccctgt acgcaggtat tccaagccgt ctgccaaagt atatgccatc tcaatatcag
    17581 cagtagcgcc tgcttcctgc atgtgatatc ctgagatact gatgctgttg aattttggca
    17641 tgttcttgga tgtgaactca aagatatcac caatgatacg catactcata tctggtggat
    17701 agatgtatgt gttacgaacc atgaactctt tgagaatatc gttctggata gtgcctgcca
    17761 attcttcgag tttagcgcct tgttctaagg ctgccacaat gtagaaagcc attacaggta
    17821 gcacggctcc gttcatggtc atagatactg acatcttgtt caatgggatg ccattgaaaa
    17881 gaattttcat atcttctaca gaacagatgg atacacctgc ttttccaaca tcgccaacaa
    17941 cacgttcgtg gtcggcatca tagccgcggt gtgtggcaag gtcaaaagca acggacaatc
    18001 ccttttgtcc tgaggccagg tttcttttgt aaaaagcatt tgactcttca gcagtagaga
    18061 aacctgcata ttgacggata gtccagggtt tcattacgta cattgtgcta tatgggccgc
    18121 gtaagaatgg agggagacct gctgcatagt tcaggtgctc cattttttca aggtcatcag
    18181 cggtatatac cggttttact tttatttgtt cggcagtaag ccagttggct tcagtttcag
    18241 gcttcttacc tgatgctggc atctcgttaa tgtttatttt actaaaatct ggtttcataa
    18301 tttttacaat tttttaaaca cagaggcaca gaaacacgga gtttcacaga tatccgttta
    18361 ttctccttcg gatgccatct tttaatgcag gaacgttgaa attgatcaaa agtccaagtt
    18421 ttttatctgc taatcgtaga taggtaagta attgaacctc atgaactggt agaatctctt
    18481 caactgattt tagctcaata actaattcat tttcaaccaa aatgtcaata ataaattctt
    18541 tgttcaattc ttttccttta taagaaacag gtaatttgac ttggtttata gctttaatat
    18601 ttctattttt tagttcttcc ataaggcaaa tttcataaac agactctaac agcccaggcc
    18661 caagttcttt atgaacttcg atagctgaag ctattacaga ttgagtaagt ttattgaaat
    18721 cttcctgtgt catctctgtg actttgtgtc tcagtgttta aatatttagc attttttgat
    18781 attcttttag tgtgtccaat acatttgact ttacactgat gaagttttta gctcctttgg
    18841 ctttaagctc atccatacag gctggagctc cggcaataac aagtattgct ttgcttccca
    18901 ctgcttctat agcttctgca ccaaaggttg catattcgtc atcgctgctg cacagcacaa
    18961 tgatgtctgc ttttgctttc tgagcagctt caacaccttc agcaacagtt ttgaacccat
    19021 tgttgtcaat cacttcaaag ccgccaactg caaagaagtt gcatgagaat tgcgaacggg
    19081 caaggcgcat ggccaggttg ccaatagtga gcataaatac tttagggcgt tttccgctac
    19141 gttcggttgt taacctgaga ttttcaatct cattagcttc tctgaaacga attaatggct
    19201 taacaacagg tgagcttgaa agtatttgct tttcatcata gatttcagcg tcaatgttgg
    19261 caagttgttg ctcagtgaag ttaggatatt ggttagtccc caaaagggtt tcgcgacggc
    19321 tcgaaatgtt tgccagccgt ttttgggcta cagcttcaat agcactttgt atagtacctt
    19381 ttttaacagc ttcgaggaag ccgccttcgc tttcgatttg ttggaaaagt ttccaggctt
    19441 cggtagcaag tgaagaggtg atgctttcta cataatatga gccggctgca ggatcagcca
    19501 cattgcccag atatgcttct tctttcagga tgatttgaac gttacgggca atacggttcg
    19561 agaaactatc agcttttttg tatatttcat caaaactgcg gactgaaacg gagtcagccc
    19621 cgccaataac agcgctcatg cattcggttg ttgcacggag catgtttaca taagggtcaa
    19681 acgctgtcat gttgcgttgt aatgttttgc agtggatgta tgcttttgct ttgtctatat
    19741 tgcctgtcca ggcttcagca actttagccc acaataaacg gaatgccctt attttagcta
    19801 tttcaaagaa atagttactg ctggttgcca tattaaacct gatagatgca atgacatctt
    19861 cagcagaaaa ttttgaaatt gctgtgattt tcgatatgta ttcattggca atagcaaggc
    19921 tataagccat ttcctgaaca atggtagccc cggattcctg caaaagggct ccattgacgg
    19981 tgattacttt gtatttagga agctgcgttt tagctttctc aatgaggttg gccgctacag
    20041 ccatgtcttc tttttcgctt tttacccaag cgcctttggt cagtagcatt ccaacagggt
    20101 cgtactgcaa tgccccatgc atcaggttaa tgtcgtaccc gtttttggct gcatcatcaa
    20161 caatgagttg atataaggtt tcaatttgtg aacaagcttt tacggaaaag caaatgcagc
    20221 caattttaat gtctttggta agggtttgcc acttttcaga ggtgatttct tctttacaga
    20281 aaataaaatt gatggctgtt gcgcctcttt caactgcttc taacgcaagt gaattggcgg
    20341 tttttacatc ataaactttt atgtcctgac agatttccca gaggttgtgg ttgggtttat
    20401 tgccccggat ataagggaat gttcccggaa tagcgttttt ctgggctaaa cccgaagtat
    20461 ctgcagcagt gtagtaaggt tttacattaa tgccttctat tgttttccag ataagttttt
    20521 tatcgtagtc ggcacccttg aggtcagcta ttatgacctt ttcccactct tcaacggaaa
    20581 ttggcgggaa ttctttaaaa agttgtgatt cttccatgcc tgtgtttttg aaaaataaaa
    20641 tgcaaaagta gtatttttat tcattttgag atgttttatg caggaaaatg cttgataaat
    20701 aaagagatat tatttttttt atggttgaag taaagctatt ttatttttta acatggattt
    20761 tattaatttt attcaacttt aaagaagtaa tattgtttta aatacaaaaa ggctatgaaa
    20821 gttaattata gtgctgaaaa gctaaaaaag atgatagaaa aggccattga agaccatgaa
    20881 atcactcgtg atgagtatga tatgattatt catatcgcca ctgaagatgg tcatattgac
    20941 gatcaggaaa aagccctgct tgctgaactt aataatatga tcgagaataa gatggttaag
    21001 tttgtggcaa aataaaaaaa ccttccaggg gtataaaatc ctgggaggtt tttgagcttt
    21061 tttattaaat atcgaattat tttctatttc ccaaaataca ggtttagccc tatagcgcct
    21121 atcaaagtgt gcttgccata agtttctttg tatgcaatgg cgttgcgggt aaagttttta
    21181 tcaaaatctt cataagctga atattcaata cctgcgttga tttctatcat ttcgttgagc
    21241 ttgtatctgg caccaaaagc tacgctatgt gcgttaagct caaagcccat gtctgattga
    21301 caaccggcag caatgccagg ttttgaatac atataaccgg tactcaaagc caatttttcg
    21361 ttaaggctat actcaaaacc tatcccggct tcaataccat tgccatcaag gctgtcctgg
    21421 cgtcctgacc agttggcttg tttgtcgaag aaataatcgc atgaaacgga aaccaacaat
    21481 ttttctatag gtttgtacgt agcacctaac gacagggttg aaggcatgtc taacctgttt
    21541 ttctggccat taggaaattt ggtaattggt gttccatcag tgttaaaacc tgtaatgaaa
    21601 tcttttttgg tgtcatattc cagttcaata ttggcctgat gggtatattt caatccaatg
    21661 tttaattggt catttggctt aaaattgata gctattaccg gggcaaaacc catacctgtt
    21721 cttttggtat cagcttcctg atcgccaagt aacatggctt ttgcaccata ttgggcagct
    21781 gcaccggtaa aaatagcgtt tgcacttgat attggagttg cagctggata tcctaaagct
    21841 gttaatgccc cttccagttc agctctattg gttgccgaaa tataaccagc accttctacc
    21901 tgtgcaaaag tcattgcgcc agctccgtta tcgataaggg cttgacttcc tttagcagca
    21961 ttggtaaagc ttgtggctgc accctgcata atagcatcag ctcttgtcca gttattgggg
    22021 ttcttataat aattatatac ttctatattt ctcaaatagc ctttgtatga ttcagaggca
    22081 tacactaatc ttccacctac tgcaattgaa agctggtcgt taattttgta agaaaagttg
    22141 agctggtagc cataaaatac tgaagttcct tcaaaatgaa tatctgccct atatccatta
    22201 acgccttgag gagctaagcc tggcactaaa tcggtaagac cgtattcaaa ggctggtaag
    22261 cccttgtcat aagttgcgcc gccaccgccg ccggcaataa agaaggctcc tgagatggta
    22321 aatttctcag ttttgtatcc tgcataaata ctggggaaag ctgggacaaa tattttgccg
    22381 gtataactgc cgtcatttaa tgcagggtaa gtgcttttta tagtcctttc ctgactgata
    22441 acctggttgt ttaacgaaac aaaaaggccg tttttgggca aaaagtttaa agcagccggg
    22501 ttgtaataag ttgcatcggc atcaattact gcatttcggg atagcatacg tgcccattgt
    22561 gcactgtggt tcgagtttgt tacaaagtcg cctgcaaata atcgggttcc cataagaata
    22621 gctgcagaca caaggaatgt tctggttttc atttctattt attgttaaat taataatgta
    22681 agccacttta agtggctttt ttgttctggt ttaacatcaa atataaattc tttttttaga
    22741 ataaaaaatt ttgtctgaaa aaataggcat attcagatgt atggttaaca gagagaatga
    22801 cggagcttta gtttaatatt agtgtctttc ttaaattcag cctgatgtcc aaaaaatcct
    22861 tgctttaggg gatatgcagc attttcttca gggttatttt tgttttgcta tattgatttt
    22921 gtattaacct aaataaatta attatgaaaa accatttgaa atttttagtt atggcggctt
    22981 ttactgccgt ttttgttttt gcatcatgca gtaaagatga tgatgataat aatacttctt
    23041 caactgagta taaagctcct tccgtagccc atcaaaacta tgctgtcgag attcctgaag
    23101 ctttggctgc taaagtacag aacgataatt atcttatggg gcttaccggt gctttacagt
    23161 ctgtaaatta tcttgcccag gcttgttttg cccagtttcc tattgataca aaagaacttg
    23221 gaaacgcttc gtctaagtca aacagcgatg gctctgttac ttattcagtt tctatacagc
    23281 aagggatatt ttatctgacg tatttttatt cttcagcgaa atcttcatgg actgctgagt
    23341 ttcaatatac aggaacaaac agtaaaacga agatagctta tgttgaggaa accggaaatg
    23401 ccgggaaagc cgaatattac tatgattctg gcaaagtggc cctcaaatat ttctggactg
    23461 atgaaaaaac aggccaaaaa attgtggtta caggttatga tgaacagggg aaagtggatt
    23521 tgacctggaa tatcgtttca aatgatgata agtctggctc tatgaaagct tatgatgctg
    23581 ctaatgtact tatttacgat atcacgtgga aagctgatgg tacaggaagt tatactatta
    23641 attctgaaga cggcactatt aaagggacat tctaatgttt tttaaggtta aatgaaatgc
    23701 ccggctaagt ccgggctttc gtttttataa cgagatgaat tcgctcaaaa tcatggctgt
    23761 tgctccccat atcttttcat ttttgtacaa aaaataaggt atttcaacat taaggccttt
    23821 gtagttttta gtttctttct tgattgtatg agggttatat atgtctgcaa caggtatttc
    23881 aatgcaatat gcaacttctt ctttttgaac ctgccaatta ggctttgatt caacccgccc
    23941 aatgtaagga aatacttcat aatggcttac cggaataact aaaggcgtta aagcccctat
    24001 aatatgtatt gattcttgag gcactccaag ttcttcgtaa cattcgcgga tggctgtttg
    24061 ttccagtgtt ttgtctgcat tgtcttttct tccgcccgga aggcttatct gtcctccgtg
    24121 aggttcatct ttgtcagtgc gcttaattag tactatgtgt ggttggccat tctcgggaaa
    24181 caataaaatt aatactgctg ctttggtcaa ttcttgtgtt acctgaaaca agtgccgatg
    24241 cgacgggctc atcttctgtt gtgcagaaat accgggtaaa ggctgtttga gcttttctgc
    24301 taaatcttca atatattttt caaaatttcg cattgttttt aaacacaggg tcacggaaac
    24361 acagagaaac acaaataaat tctattttaa agaataccag atgtttaatt tggaaaaccg
    24421 atataattca attttatgaa aatacttgaa ctacagaaaa actttctctt ctgtgttttt
    24481 gtatctctgt gttctgtcta taaatatttt gaaatggcgc tttccagctc cttataattg
    24541 tgtaccactg gtaattcagg ataatcatca attaccttct gaggaggaca atacaaaatg
    24601 ccatgatctg cttctttcaa catgctaatg tcgttgtatg agtcaccaaa agcaacaact
    24661 tcgtacttta gctgtttcag ggcttttgta acttcccttt tttggtcagg ttgacgaagg
    24721 ttgtatccgc aaaccattcc cttttcatca acagacaggt agttgcagaa aagtaaagga
    24781 tatttaagct gcttcatcaa tgggctggca aactcggtaa aagtgtcaga aacaatggct
    24841 atttggcttt tgctcctgat tttatcaagc atttccaatg ctcctggcag tggttcaatt
    24901 tttgaaataa catcctggat atcttttaat gtgatgttgc gttctttcaa gattttaatc
    24961 ctgtgcttca tcaatacatc gtaatcactg atgtcgcggg tggtgagctt taattcttca
    25021 ataccggtaa tgcgtgcaac gttgacccac acttcaggaa cccatactcc ctctaaatct
    25081 gaacagaaaa ataccatggc tgttatctct ttaataaata tgtcttaaat gcttcgtaat
    25141 tgtttggcat gtcagtcatt tcctcggttt tgttgtcgag ttgcgaaaga ctggcgggga
    25201 gttcaggctc aatgttgaga attgacttaa tctcttgtgg aaacttagca ggatgtgcgg
    25261 tttctaatgt tactgtcaac tggtaagggt ttttatatga aggattttca gcataaaaat
    25321 catgcaaccc cttccatcca acagctccgt gtggttctaa aagtagattg tagctgttat
    25381 aagcttcacg tatggtttga cgtgtagctt cgtcagtcac actgaaggct gtcatgtcgt
    25441 ttttgagctt tgcaacatca ggttctttca ggatattgcc tttttcgtcc atttgtccgc
    25501 cataaagcgt aatgatacgg gccatgttgc ttggatggcc aacgttcata gcgcttgaaa
    25561 tacagttgcg cgatggctcg atagttttgt atgacccgct acggaaataa accgggactt
    25621 cgtcgttttc attggtagca ataacaaact tctcaacagg taatcccatg tgtttggcaa
    25681 ggataccgcc catgaggtcg ccaaaattcc ctgaaggtac cgagaatatt atcttatcat
    25741 ctgcattgga tgccaattgt gaccatgccc aaaaataata aacactttgt ggaatcaaac
    25801 gacctatgtt tattgagttg gcggaagaca ggtcgagttt ttgcaagtct ttgtctgcaa
    25861 aagctttttt taccatcgcc tggcaatcgt caaacttgcc atctaaagct attacagaaa
    25921 tgtttttgtg cagtgtggtc atttgtttgc gctgacggtc tgtaacctcc gtttttggaa
    25981 acaaaataac tacttcaata ttgtctaacc cataaaatgc attggctata gcgcttcctg
    26041 tgtcgcctga tgtagcagta aggataagca ttttgcggtt atccttcttc aggaagtact
    26101 gtatcatgcg ccccatcata cgagcagcaa agtctttgaa agaagcagta gggcctctgt
    26161 caaggcgcat gatgtagttg cggtcataaa cctgttccag cggaacatca aagttgtaag
    26221 catccttggc aagtgcaaac aggtctgctt ctggtatttc gccctggagg aagttgttga
    26281 tgacggtata tgctatttca aaatagttct tttcggaaaa accccaaatc acgtctttgc
    26341 tgagtgttgg aatatagttg gggatgtaaa gccctttgtc gggggcttgt ccggcaatca
    26401 atgcttcgcg aaatgataca cttggcgaac aatggtttgt tgagtagtat tcgatttttg
    26461 acatgtgaaa ctaaaatttt agccctgcaa aataaatgaa tttcctgtta ttcaggtgac
    26521 attaaatata aaatgatttt ttgtatttaa agaaacaata gccaatttca atattctatc
    26581 gccaatcatc aataaaaatg agtacctttg cagtttaatg taaacttttt aaataaagaa
    26641 agttaatgag taaagtattg attatcgggg ctggcggtgt tgggaccgtt gtagcccata
    26701 aggtagctca ggtaaaagac gtttttaccg acattatgct ggctagccgc accaaatcaa
    26761 agtgcgatgc cattgccaaa gctattggcg acagccgtat caaaactgct gctgtcgatg
    26821 ccgaaaatgt cccacaattg attgagctta tcaatagctt taaacctgac atggtcatca
    26881 acgtggctct tccttaccag gatttgacca tcatggatgc atgcctggct acaaaaacct
    26941 cttacctcga tacggccaac tacgagccaa aagaagaggc taagtttgag tatagctggc
    27001 aatgggctta tcaggaaaaa tttaaacaag caggtattac tgctattctc ggatgtggtt
    27061 ttgatccggg cgttacgagc gtttttacgg cttatgctgc caaacatcat tttgatgaaa
    27121 ttcagtactt agatattgtt gactgtaacg caggcgacca tggcaaagct tttgccacaa
    27181 attttaaccc ggaaataaat atccgcgagg taacccaaaa aggtaaatac tgggaaaatg
    27241 gcaaatgggt tgaaactcag cctcacgaaa tacacaaacc tctcaattat cccgggattg
    27301 gacctaagga atcgtattta atatatcacg aagagcttga gtcattggtc aaaaactttc
    27361 caacccttaa acgtgcccgt ttttggatga cttttggcca ggaatacctg acacacctgc
    27421 gtgttatcca aaacattggc atggcccgga ttgatccggt tatgtacaat ggtgtagaga
    27481 ttgttcctat ccagtttttg aaagcagtac tgccaaatcc cggtgatttg ggcgaaaact
    27541 ataaaggatg gacttctatt ggctgccgta tccgcggttt gaaagatgga aaagaaaaga
    27601 cgtattatgt ttacaataac tgtagccacg aagaagctta taaagaaacc ggtacacagg
    27661 gtgtaagtta tacaacaggt gttccggcta tgattggcgc tatgatgtat cttaaaggct
    27721 tatggaaaaa gcccggcgtg tttaatgttg aggaatttga cccggatcct ttcatggaag
    27781 cactgaataa acatggcctc ccttgggtag aagtgcataa tggcgacttg gaactataga
    27841 aagaaaatat tgacttaata tttactcccg acgatgcaat ggcattttcg ggagtttttg
    27901 tttgagtgca cttcattgtg ttgtttcttc gaaagaacaa cacaatgaag cttaaaaaac
    27961 accttcgccc taaagggctt cgataagcga aagcggagag agtgggattc gtaacccctt
    28021 aataactcct tatttttcaa tattttataa ccttacgaaa tttttactca ccgaattact
    28081 cactattgtt ttgaagcaat ttggctgcaa atcgtattac aatattacaa cttttgaggg
    28141 acttttgaaa gtaaagcaga ctattttttg aactggataa cagaatagtt aagggttgta
    28201 agcgtgttct atcttggttt ttaagaaaga atacactaac tgaccaaaag cggttgaacc
    28261 tgttctgcat ccatgcctgt gaactggcaa aactcataaa tagtaacaaa atgatgctgt
    28321 tctttatcga gttgctgccg tatctgcttt agcaggtttc tactgaactt ttcagaacgt
    28381 ccggttatgg tcattacatc tttcggatat attacaatcc tttttgtcat gccctggtat
    28441 aaaattatgc tggccatagc tttaccatga ctttaatatg gctttactat gactttgata
    28501 cgaagataca cagtttaacg gaaataagcg gaagtaatcg gcaaattatg cccttacgtg
    28561 gctgcatgtg tactatttac aggtatttta atgtaatgtt gctgataatt aacttttgtt
    28621 caacccatta aaattgtaaa ttatggctaa tcagaaagga attatcaaaa tagaaggtac
    28681 tgtaggcgga atgaccttct acaaaaagga tggcgaatac ctcgtcaaag aaaaaagcag
    28741 catttcggct gataaaattg caaaagaccc agcatttgca cgaactcgtg agaataatgc
    28801 cgagtttggg gctgccggaa ctgccggaaa agtattgcgt gatgcattgc gaaatatgat
    28861 gttaaatgct tccgatacgc ttgtaacatc gagggtaacg caattaatga ccgatgagct
    28921 taaattggat gtaaccagcg taaggggtaa gcgtttacct gctaatggtt tggcattggc
    28981 tacaggaaaa gccttgttaa agggcttcaa tttcaatgca aattcgatgt taagtggcgt
    29041 gttgttcaaa ccctatacgg ttgttactgc aacgggtgta ataacaatca caggcttaaa
    29101 ccctgctact gatattgccg ccccagccgg agcaactcac atgagtatca ctggcggtat
    29161 ggcgaacgtc aactttgcaa ctggggtttg ggctgcaagc cttaccaacg ttcaaaacct
    29221 tgccttaaat gcggctgctg caaccgtaac attgacacca acggctgttc ctgccggaac
    29281 gggtattaaa gtacacctgt tgaaaattga gtttttccag ctcttaaatg gggttcaata
    29341 tccgttaaag aatggttctt tcaatgcttt ggcagttgtt gaggttgcat aatcatgaat
    29401 gagtttcaac tattcgggat tattatctcg ttgcttctgt cggtggtggc tttctttgta
    29461 aaacaattgc acagggattt taagcgtgtc gaaaaagacc tgggcgaagt gaaagttacc
    29521 acctctctca ttaaaactga attcaaagga atcaatgatt tgatgaacca aaaaattgaa
    29581 ttccttgaaa agaggcttac gcatatagaa acgttcattt ttaaaactat aaaaaaatgg
    29641 caaagactaa tttaacgttg gttcagcgga ttaccgcccc aacaccaaaa atattcagga
    29701 taattaggac tgttggcctg tctttaaccg ctgtcggtgg cgcaatactc gcagctccgg
    29761 caatgcctgt aatactggtg accattgcag gttatttgtc ggctgctggt gctgtcatgg
    29821 cagcagttag tcaggttgct gtggatgggg attaaacgcc atccaaacct catcagaggt
    29881 actttaaaag cttggagcga aaaactctag gctttttttg tggctacacc tcaaccatgc
    29941 ttgttaaaac agcttagtgc atacgcccgt ttcaattcct gaataagctg taagtgttct
    30001 ttcatttgtt tttcttttat gtcaaagaac tttagttttt caactgcacg aataaattca
    30061 ggctgattta cctttactac ctccattttg tcgtttattg cttttttgta atcccctatt
    30121 cggataaacg gaataacaga ccctattaaa tacggatgaa aagcttttgc cttccaaagt
    30181 gcaaatggta cccaatataa tgtttctttt aattcttcgt tttcagcctc aattttaaaa
    30241 caattgggac aagcttgcaa aagtggctta cctgagttta accctttatt taaagcgaaa
    30301 aaatgaggtt tggggctttc ttctttcacg gtcgatgatt ttacagtaaa tgtgtgcatg
    30361 gtttcttcga taaatatttt gctcgcttcg cttcgcacac atattttcaa agaagaaaga
    30421 aaaaacggaa aaaaagaaaa aagaaagcct cttttcaggc atgacggggc ggcctgtcaa
    30481 ggttttttac gaaaaatact acccgacaaa ggagggtgga gattttttta aaaacccgaa
    30541 gggcatgacc tttactggtt gcccctaaat gcctaacttg ctgcactttt ttgatttttg
    30601 tgattgtccc tgctttttct gtccgaatgc acaaaaaaat aaagtaagcc acctaaaaag
    30661 gcagcttacc aacagagaat tgcagccaca agagctgaat agaaaccaac ggaacggcac
    30721 caccgatata agaaaagtgg aagcgaacct tactacaagt gtaggcaacc aaccaccagc
    30781 cagccaacct acggcgaaaa atacagttaa acataatgcc tgtatgccac aggcggaaag
    30841 gcaaagatgc aagggcagac caaaaaatac tctttttgtt ttgacctttt gtttttcggg
    30901 caaaagaaaa aggaagattt tttgaggctg gcgcagcgaa cccttgtcag ctttggcttg
    30961 aagtcctgta actttgcatt ttgttttggt gtatttgtta aaacattgat attgacttat
    31021 ttttcttttc tttttgtttt tgtgggtcgt aaaaagcagg ttataacagc catgagctaa
    31081 aagggcaagc catagggtgc aatttttctt tgcacccgac ggcttgaccc tgcgaagcca
    31141 ctgcgagttg agcaagggtg gtggacaaga aggctgaggc acgaagccga tggacggcac
    31201 atgtggaagc ggaaggctgg cggggttttt gctttttttt gcaaaaacca tgacagcctg
    31261 gagcgcacta cacgaggcta ccgctttaat agccagaacg aagtggaggc aattgaataa
    31321 agtcagccga agcccgtagc gacccgcagg cgagctaaac aatgcagccg gtggactgca
    31381 aaaggctgaa aagaatggag gcggaatgaa atgtaaggat gctgagagca gcgaagccct
    31441 gaaatgtaat gtagccgtaa tgagtgaagc tttattgcag gatagagtag gctgcaatga
    31501 aaggaagcaa ggcaatgaaa agcatgacaa gctgtgaagt gcaacggaac acccgaaggg
    31561 cggcacagct cttttttata cctttaaaaa ataagcaagt ttataaaaat gtgctgtgac
    31621 ggcttggcgt gtctttttgt attgccttga tggagtgaat accatataac ctgcttttgt
    31681 ggtgggcggg tgaggattaa aaagagtttc attccacttt tatcaatatc cgacatttat
    31741 ccgccaaagt gtaagtatat ccgacattta tccgccaaac actaactgat atttagagcc
    31801 ttttttctca cctattcttt gcagaatgcc tttttcaact aaatcgtcta agtctctaag
    31861 ggcagttctg tcagaaacag aacatagttc tatatattcg ctatttgaaa ttgaatgctt
    31921 tcccctgata aataggatgg cttttgtttg gcgttcgttt aaattcagtt tctgtaaatt
    31981 ctcaactgaa aatctatctt taaataattc tgagagaaaa ccaccgctta gttctttaaa
    32041 ttccggttca ggaagattgg cgtttttaca tgcctccata attttgatag taccacgccc
    32101 ccatgaatca atatagccgc ccttaaaaca aacatcagca attatttggt ttctgggttt
    32161 tgaagcatgt tggcgtttca aggcttcgaa cgtaatacta tcgggaagta ggccatcatt
    32221 ccaaatacta attttatcat tatacacacg aatttgtgta tgagcaccca tataattgcg
    32281 atgtaccagt gcgtttaaca acatttcacg aagtgcagga aggggatatt cgggggtttc
    32341 aatcctgttc ataccttcaa agctaacctg tgaaataaga aacttatggt ttaactgctt
    32401 aagcacattt gtcaagtaac ctgattatat taccttcctc ctgttcctga aacataaggt
    32461 cagtttcgga attgccaaaa cgaccaattt taacaaacat gctaggataa aatttaccgg
    32521 ggtctttgcc gaaaagaaca atagcagccc gttttaactg attgttatct gataaacgaa
    32581 gcttatcaag caattcagaa atactgaggt tttcaaaatc aggcaagcga cctgcctttt
    32641 ccgaagcttt taaatacaat tttatagttt gttcgtcaat atcggcaagt gttgcacgag
    32701 gttcaataac atcatcccag gttttgcccg atttctttaa aagaaattcg tttaatgcag
    32761 tacctattaa ttcttgcttt gtactgccac ttctgtaata atagcgacca cgcaatgaaa
    32821 tggggactga gtatggagga gtattgattt caatgtattg caaactatct gattccaata
    32881 aattaacctc aaccataatg cccatattgt tgcgtatttt attgggaatc tcgtccatta
    32941 aagacttgta gtctgccaaa ccaattactt tgcccgaatc gtccatgcca atatatatag
    33001 ttcctccctg ggcatttgcg aaaccacaaa tccatttaag gtagtcgtcg tgccaattcg
    33061 atttgtattc tatgttttgt tgttcgggca ttgagctttt tcgtaattta ttttttacaa
    33121 agatattaaa atggatttta gccgaaatat tattttcgca atctcatggt ttgtgacaac
    33181 actgttgaca caaattgaga ataatactgc caaatattca taatgggtaa tttcgcagaa
    33241 atcgtctgcg aaatgttttg agtatttaca aatcattgaa gttaattagt gtatagctaa
    33301 acgttttacc ccagtatttt tgtgccattt cgcaaagtga aatgaactcg ctgaactggc
    33361 cggaatcggc aaagacctgg caacctgctg accattggtt tacattgatg gatttacctg
    33421 ttaagtttgc acggtgaatg tttatgccga atatgcctgt ttcttcttta ccgcttgtaa
    33481 aatcgagata attgtttttg ttaaagtcac ggattactgt tactggcata aattgaacca
    33541 atgctttgta tttgccctga tgtaaaccaa ggcaatagca atgaaaatac tggtccgctt
    33601 tcaatatagc tgtgccttgc gggcttaagg gatgattgag ataatacaaa ccagggtcgg
    33661 ttgttgcttc gtatttgtgg aagttccact gacctttgta cttccacatg ataacgatgt
    33721 aatcatcgaa ggaatttggt acacggttat tgtttcttat cccgataatg ttgaggttat
    33781 atggtttggt gtcagtttca aatatttcgt agcctttatt tttaagggct gagtaaacgg
    33841 tgtcaaatgt tattgattcc ataatttgaa aatgtattaa taatttggtt aaactctttt
    33901 gtcttttgcc gattcgctga aagcgatgag gttgaattgt tcacgcaagc ggctgcgaat
    33961 tcgtgcgccg taaatatctt caatttcgct actattgagg tttgtagtta aatgtgtgaa
    34021 catttgacgt gaaacaaata attcgtaccg tgaaagaaga atttcggcca ttacgttgca
    34081 gtcgttgcca aagtatttca ggctgttttc ggtgcctaaa tcgtcgaaac aataagttcg
    34141 aggaatgtcg tttttgaatg attgtttgct gtatttgtgt attaccgaat agccttcttg
    34201 tataaactca aaactcacat cacgacaggt tttcattaca aaacgctctt cgagtggttg
    34261 aatatgccgg ataatgttca ttagtgaagt tttaccacaa ccaacagggc cagaaagtaa
    34321 aatacctttc cgcaaggaaa gtttgtgttt ttctgctccg gcattgtctt tgagaatata
    34381 caccagcagc ttgtaaataa tttcgtaatc ttcaggaatt actttaaaac ttggatggta
    34441 cagttttttg cctgtagatt cgataaaggt taggcctttt gtaaaatcga aagtcataat
    34501 gtgctggttt gaaaatatga aaatgattta aagaggttcg ttgtagtttt tgccgtttgg
    34561 cgatggttgg ggctttgatt gtggttttgg caaaccctgt ggggctataa aatgctgtgc
    34621 atttagcatc cagtttcgtg cggctgcttg ccagtttttc atgggtgttt tgcccccaac
    34681 acgccaaccg ttgctttcga aatggttata gaatttattg gcctcaacta ttgggaactg
    34741 ttcggtatta aaaaatgcta ttacttcttc catgattggc ggtattgaat caggcttttc
    34801 tttttttggc gcaacttttt ttctttttga attttggctt tgtagcatac cggggttatt
    34861 gttttccgaa aaaggtaaat tctcattttt ggaaaattgt gtttgtgagt tcgcatcact
    34921 atcgtttatt atgtttaata agtttgtatt gtttatagaa ggtactaatg cttgttcagt
    34981 gcctgtacca gtagtagttt cgttacctga ctgatttttg aacggctgcc cgtctgtttg
    35041 ctgaactggt ttgtagtcgg ggtcgaatat tacaagatga accaaactgc ctttgaaagg
    35101 attgtatgat ggcatgtatt ttacatatcc gaaattgtgc aaatctttca tacacttgtg
    35161 gtaggttgct ttcgaacata ttttactaac cttcatggtt tcttcacggc aaatgcttat
    35221 cgggttctgg aaacggttta tattccaaaa ctgaaataaa gacaggtaca ggcttatatg
    35281 tgtggggtta agcctgtcgt cgggaactac acgatcgaaa aaaccagtaa ggtgttttat
    35341 gtagttcata atccactctt gcctttttgc aaattggaag gatgtggttt ttgattgttg
    35401 atttgatttc cgttaattat cttttcaatt tcgttgtaat tgtaaaagat aatactacct
    35461 atccgggtaa atgacaatgt gccattaaca cggagatttt gcaaagtacc aggagagatg
    35521 tttagcagtt tacgaacttc ggcagatttg agccattgtt tttgttctga ttggcaacct
    35581 tgttctttca gaattttttt aaggtcttcg agaagttctg tcttaaactc ccgcaaatcg
    35641 tcggttgtaa taatagatgt aggcatactt tttaaaaaat ttggtttcta caccaaaatt
    35701 caaaagatta gcccaaaaaa agcccaagtt atacccaagt tgggtacaca ttaattttga
    35761 tatgttttga cacagaataa tataactttt aggctctgat gtgtcaaaaa tgataaaata
    35821 ccaagttggt atcatcttgg gtgttcgtca tcctgttcaa tttttttgag caatgccgtt
    35881 tttaatgcat caaggaactt ggatggattt tggcgttgct taatttcgag gtaggtacgg
    35941 taaaagtcgc ccaagtcaat atcaaaaaca gcttcgacaa acagggctat ttccttaata
    36001 tctattaagc cattattaat gctgttacat gcttgcaaag cataaataag ttctacgagt
    36061 gcaattttag ggtcagtcca ggcatattta ctttttcgca cctgatctgt tcttttaaat
    36121 tctttgcggt caattaatgc cagttcagtt ttgaggtata cacaaagcaa atcattagct
    36181 aatattttgg caactttgaa atcgtggctg gtggagaaag cggggtcggc gtcgaaataa
    36241 aatgtatcga ggtaaagatg caaatcgtac ttacccctga cgaaatattt atcgtcgaga
    36301 tataccaatt tgttacgcat atattggtaa aattcgaggt tctcggcaaa gaacttttcg
    36361 agcttattga gttcattttg caagtatctt ttttgagatt catcgctccc gtttggccgt
    36421 tttgactcga tattgaaaat ctttataaag taaattagtt tgctgtaaat tgccggtttt
    36481 gtttctttga aaaacaatat ttcggcttcc tgatttggaa atttgttttg acttacgaaa
    36541 tctttcaatt cttttaaaca ggcacttata cagacaattg agcgttctga tatttttaga
    36601 atgttagaaa actcactctc gattgttttt aggtcatcat caagattttg gcatatattg
    36661 caaatgtggt cactcataac ttacaactga tttttctatt gttttgccct ccctaaattc
    36721 atttacttta tagcttttat tttgcccaac gaggcgttgt atcaaattta cagttgcttc
    36781 gtcatgaata ccaggaataa acgtggtgcg aaattcgcct tcgattgagg tattgttgag
    36841 aagaagaatg gaaaccatta tcttttcaat aatttcatct gtcatttgaa ttccaatgat
    36901 tttgccgtac aaatcttttt cgggcgcaat ttttatgtcc atagcaatgt aatctactag
    36961 cttgttttca acgatttgct ccagcatgta tgggttcgat ccgttggtat cgagtttaat
    37021 ggaatagccc aaatctttta attctttcag aaataatggt aaatccttgt gaatggttgg
    37081 ttcaccgcct gtaacaacta ctccatcaag ccaatttttg tgttttgcaa ggtaacttat
    37141 tatgtttata ggagctaatt caagatgttc aataaaaagt tcaggtagca caagctgcgg
    37201 gttatgacaa tacccacaac ggaaattaca gccttgtgta aacacaacgg ctgcaatttt
    37261 accgggatag tcaataagac tttgttttac gaaaccacct attctcatag ttttttatgc
    37321 ttttttagtt tttcaaaccg agtaggcaga attcctaatc tattattgag ccattctgaa
    37381 tatatatccc aacacagcaa aagaggtgca gtatttggct tacatggttt tcttttcatg
    37441 gtaatcagtt ttttagctgt taacattcaa tgccaattct tgcttcaacc ccttgagtgt
    37501 agtagtgttt tacctctttc atttcggtaa ctagatcggc catgtctatt agttcctgtg
    37561 gggcatagcg tccggtaata atgatttcgg tttgttcggg tttctttttc agcagttgaa
    37621 tgagttcatc cactgaaaac aagttgtaat aaagggcaat gttggcctca tcgagaatta
    37681 ccacatcgta cgtgcctgac aagatgatgc ccgaaacctc tttaaagcct tgccgggcaa
    37741 tgtcaatatc ggcctgagtc gggcttttta ctataaaaca ttccaaacca aattgcttaa
    37801 ttgaaatggt tggcagataa ttaaatacag cctttacctc ggaatatacc tgacctttaa
    37861 caaactgggc aaaatatact tttttaccag caccgacagc ccgcaatgca agcccaaaag
    37921 cggcagttgt ttttccttta ccgtttccgg tgtaaatttg aatgtaccct ttcattttat
    37981 tgttgttttt aatgtgtcaa tttttgtttc gtttaaaaat gctatgtgtc cggggtttaa
    38041 cccggcttta aaagaatact tctttttgcc ttctttactg aaacaaacta cattgccagt
    38101 gtttacaaaa ccgtgcgcat cggtaatgaa tacgttgccc gattgcttat caaccgtaat
    38161 cccgtaagcc gtttctatta cagtaccatc ggtaatgaaa ttgtcgcgga tgatcgtttc
    38221 gtttttaaca ttaataagca ttatttttga accggagccc gtcataaagt catagtaata
    38281 aacataagca gtatcaccat ttatagcaag gttcaaggct tcaaaaccgg ggaaggtata
    38341 ttttagctca tcggttttac tgtctataat ttgaaggcac atttttacat caccgtaatt
    38401 gcccctcgaa atcacatata aatcgccata tttatcgggt acaataatat aagggttaat
    38461 gcgtaccgtt attttctttg tttcaacaag cgtagcaaca tcaattacag aaacagtatt
    38521 gtcatagttt ggataatcaa gcccgccgga atttgacaca tacactttat tattggcaac
    38581 ggcaatcccg tcgggattac ggcctacggt aatgtatttt tctatttcga gtgtagccgt
    38641 gtcaattacc gcaaccgtac catcgaacga acaaacaaat gccttgtttt tattaaaagc
    38701 gatatatcgg ggctggcgag gtttgtcgcc gttaaaaaac ggtatttgtt tgatgctttt
    38761 accagtaaat gggtttacta cttctaactg gctcgaaaca ttcacgatga tatacatttt
    38821 gtttccataa atggcaatat cgttccctgt gtcacccagt ttacggccat tctgcaaatt
    38881 gaaataatcg gtttggggca cagaatcagc aaaactgtaa taggtaagcg ttgaattgtt
    38941 catattgaac aatccttcgt ttaacacata tagccctttg gtggtatagg ttgtatcggt
    39001 tacgggtttt acaaccggag ggtcaacaac aggttcaatc gggtcttttt tgcaagattg
    39061 tgttatgaac aatagtgcaa gaatgggcaa tattatttgt ttcatatttt tcatttttaa
    39121 aatagtaaag ttaaagctac ggtgtatgag cgtccgggca tcgggaaact tcttattacc
    39181 tcgtattgtt tgtttaaaag gttgtttact tcacctttca tctttaaagt gtaatctttt
    39241 attttaaact cgtacaacgc agaaatgtcg ctcgtccacc atgacggaat catgttttca
    39301 taaatatttt cgcccaacac ataacgatag ccattaaaca acgtgttgta gcttaccgag
    39361 aatgatttgt aggcgacagt tcccattgcc gtgaatgttt cgaaagggat atagggtatc
    39421 tgctggttgt aggttgccga attttcatcg gtaacatcgg ttgctgcctg ataggtataa
    39481 ttgcacgaca acgaatagcg taatcctaaa acaggtttgg tttgcaattg gccatgcaat
    39541 tccatgcctt ttatatcgac taacccgatg tttcgcattg accaaacaaa caggttcttc
    39601 gtaggaacgg cgactatttt atctttcacg tggttgtaaa agaaatcggc tttaaacgaa
    39661 atatattcaa taatgcccca atgggaaaaa gctgttactc cggcattgta ttgctttgcg
    39721 tattcaggtt taaggttatt gttgcctaca agggtataat ataaatcgtt aaatgtgggc
    39781 atcctgaata cgtctttgta caagaaccgc aggcggatat tggggctttg tgttagtctg
    39841 taaccacacg aaacggaagg ggttaaaata ttacggtcgg gggctggttg tccttccatt
    39901 gttttttcac gaaccagcgt ggccaatata ttgccattta cttcccagcg aggccgtaaa
    39961 tattgaaatg ccaaaaccga aagcgaagaa taacgtgtag gcttggcata ctggtagagg
    40021 ttggcatcta gtgtgtttac aaaaaaatca gaagctatgg aaagatggag ggaatcaata
    40081 actttccagt ttaccgcttg cgatatgtaa tattcccgtt gcagatacct attatcaagt
    40141 ttcccttcgg cattcaggta ttcggggtct aaataccgta accagttttg agagtatttt
    40201 atgtttgtga gccattgcag ccttttttta gtgtcagatt tatattgaag gttggagaag
    40261 aaatccttgt tccacagccg ttgcgttgca aacggattgt aaaacacaac tgcacccggt
    40321 aaccctcttt cagaaccgta gtaatacatt tttaacgaaa gccttgagct gtctcgcaat
    40381 attgtaacta atgcagcgtt gaggttgaca gattcaatat ctgaattttt tcttatagct
    40441 gtggtatctt tgcttctgcc aagctgtata tgaaaagggt attcgccatt ggctttggtg
    40501 taattgcatg aaatatcgga atatgatttt tgccccagtc tattttgtat tgaaaacagg
    40561 ggattaatta acccaaacga accgatttta cagcccactt tgaatctaaa aggtttattg
    40621 ctaaagttgg gaatagatga attaatgctt attacgcttg ccgaggcata ataccttgct
    40681 ggttgacata taattgttgg ttgtccgata aacaacgaca cgttgtcggc gttatcggta
    40741 gaaattttac ctaaatcaac ctgccctgta gcaacatcgc taaattgtac cccatccata
    40801 aatacaccgg tatggtttgc ccccaagcta cgcaccatta ctgttttcaa ccctccaata
    40861 ccaccataat ctttaactgt aatgcccgaa aaggtcttaa cagcatcggc agcggaagtg
    40921 cctgaaatcc ggtcgagttc tacccttgta acagattgca tggggacaac cgacttgtct
    40981 ttctcgggtg gctttactgc ggttactgtt acttcttcga gttttacagt gtcttttatt
    41041 agtttttggg aaaacaagtt aaatactgaa aaagagaata atatgattaa aaataattgt
    41101 ttcatcgaaa agtttaaaag gtgccggaag ttaagctccc ggcaccttta ttttactatt
    41161 gtttcaatat tttaacagat tggctgtctg attttcctga aatttttaag atatagtaac
    41221 caggcgttaa atgttcagta gagaattgaa cggtattacc tgctgtaatt tgctcttcaa
    41281 taatccttcc gtaaatatca agcaaaataa ccctgctatt agcctcgcat tcaacattca
    41341 gatttgcaga gaacggattt ggataaactt tgacattgtt ggcaagagca ttatgaatac
    41401 ctgttgcgct attggttacg gtaactttga aagtatctgt aaccatttgg ttgtttaaag
    41461 ttgcacttac cacaatttct gattcaccgt gctggttggc caagaatgtt aatgtcagtg
    41521 tttttcccga taaatttgta gaaacaagcc ctgtgtttga attcgatgct actgctaaag
    41581 taattccaga agggtcataa gcagtaaatg cagaacttaa atcgacattt aaaggctgtg
    41641 cattcatatt gacgttcaca tcgtcaagcg gggtaagttt tacaggttgg gcatcgggta
    41701 ttatagtaag gttgtcgatg caaaaatagg ctggtgtatt catgccccat gaaccaacat
    41761 cagatgatgc gaggttgaaa cgaagttttt ttacttttcc caacttgttt aaatctaccc
    41821 atcgccaatt ttttacaatg taatccctgg agttgtcgtt agaacggaag tcggcaagat
    41881 aaaactcgat ggtatctgtt tttgagccat cttgtttttc tccccaaatc aaaagcttga
    41941 agtaatcagg gtcgttgccg gttgtgcctc caaacttttt ggcaaacata tcaccatctc
    42001 tcattgacaa ataagcatac gtacagtttg tcacataaaa accgcttaca gcatagcttt
    42061 tatcatcttt aaaagtaatt tcaacaggca tgtttgagaa atcgcccaac cagttttggg
    42121 caacaaaagc aactgcatag ttagtacccc cagtgtcggg ggcattcata ccacctttgg
    42181 tgattgcact aagcgttgag gtctcaagtt tactttggtc aatagctgtg tcgtttcgat
    42241 tattggaata catgaaccca taatcgaaat tagtatcatg aaaaaaacga aattttgcca
    42301 agccagaagc ccaactggta tcgttggttg gagaccacca ggaatctttg gctaaaggta
    42361 attcatcgag tgttgttata gaatatggtt gccctgcatt taccacgcta actgcatcga
    42421 ggtcgaagcc acaagaatga aatggggttg gccaagggtc attaacaata tgtcctttag
    42481 aatcgtgcga agcataagga gcatttatgt tgcccactac atcaaccact tttacaaaac
    42541 gaatattatt caggtcaatg ccagtactgt ctttaatatc gtctaggtca aaaggagtcc
    42601 catagccagc aatatctttg cctgccagat tgtaaatatt ggttgggtca atagctgcaa
    42661 agcctccaag ttgagggctt tgtggtgtaa gtgataccgc agggaaacgg acaaatcttt
    42721 tgccgtcgga actaacttca acaaaagcca attcagaaaa agttgtactt ttctgccctg
    42781 ctggtgtata aaaaccgttt tcataaacgg caaagtcagg gccttttcca tttgaaatag
    42841 gcctgtcgaa agaaaggata gcaactccac tgtcgcccaa acttaccaca tcgtaaggcg
    42901 agttttgtgc tttgcccaaa gcaattgcag gatagccaaa cgaagctttg tttgtgccat
    42961 tgtattttac agaagtgtcg gcaatgttta cccaacctct gtatagctca atgccagtag
    43021 cccagctttt aataactgaa gaatcggtat atatggccgt agaaccagta ttgccagcac
    43081 cgggggcata tttgttttgt gcaggtgaat tgtgcattat acacatacaa cccgccataa
    43141 aaagtaaaat ttttctcatg ttaatttatt ggttaatttt taaatattcg tttgtaaaca
    43201 gtcttcccgt ctgtagcaat ttttaacgta taaattccct tttctatgtt tgctacatta
    43261 acagaaggac tttgagtgct gtaagattct ttaagaattt cattaccttg caaatcaata
    43321 atagttacct gtgcaggctt atcggtataa atattaataa attcggtaca ggggtttggg
    43381 ctaatgttta tatcgtattc aaccgcttta ggtaaactta atgcgctgtt ttcacttgca
    43441 acagatgaag tgcttgccga ttttaaactg ccacaggctg tagctgctgt aaatggtgtg
    43501 cgtggagggt ttgcagtcca ggaaggggca taagcaaaac gccagccatc ccatgcgcca
    43561 ttggtaagtg ttctgttggc tatcccgtaa tttgagtatg tccaactggt tccgtttgta
    43621 cttaaccagt aactccaata gccttgatat agccatcctt ctttataaac atcatcggaa
    43681 tcacctgcgt ggcctgtctc atttgccccg gcaacatagg taaagccatc gcaatccatg
    43741 tcgtaaccta aaccgtaaac tgtttggtcg ccataagaac cagctttttc gaaaatccga
    43801 gggtctatag ctttgattgc attaagcata tcccggcctg ttttagttcc tgtccattta
    43861 tagccccaaa ctttggtaac ataatttcca tcgggtgttg tccattcaat taccaaagca
    43921 gctttattag ttcctgaacc agcccaaagt tgaatatcat tgaacgaaaa ttgagaaaag
    43981 gcatttattg aaaggaaaat gctcaaaacc agtgataaaa acaaatgttt ctttttcata
    44041 aaaagtaagt attaaaatta atatttgatt gattccaacc gcaaaacagg cattcggctt
    44101 tgcgcaaact caaatgcgcc aaacaaaacc ccgatataaa caaaatcacc taaaagtgta
    44161 tttttaaaga atggtagtcc ggctgcatag caagtcatta acccgctaaa actcttaggg
    44221 tacatgtttg tagaagccca aaccccaaaa ttggagagta gaaaaaatat aatagaagct
    44281 aaaaggcttg ccagaataag gtttcggatt ttaatccgct ttagggcaac aaaacctatc
    44341 aaacctatca tgataaaagc accataagtc cagtaacagc cttggtaaaa ccatacaaaa
    44401 tggtcgaaat attggctgta aacaacatta tttaatacaa ggtcgcttaa ccacatgctg
    44461 ataatgggaa tcaagaatga aaggtatcgt tgcgaaaaat aagcagcacc aaacaaagcc
    44521 attgccccga tgggggcaaa atttggagga tgaggcagca atcggctgaa tgctgccaaa
    44581 agcaccatca gggtaataac cccgtaacgg aggtgtaaat ttttcttttc tgtcataact
    44641 atcgaatttt aagtgaaaca tacacgttaa aacccgatat gcagcaatgt gaagaaggat
    44701 agaaaatcaa aaggcttaag ccggatgatt tttcgctttt ctcccgaaag ctacaaatcg
    44761 gaatatcggc aggttttctg acttgctcgc ttttaaacgc cttcccatct ttaatgacag
    44821 tggcttctgt gtttaaaagt ttttgttgag cttacagcag cgggaactat tgccgactta
    44881 cacggcattc ccttttaatc ctgtttaagc aggaaccgat atgtaattct atattctaaa
    44941 atcagtcttt tatcatttta tcaaaaagtt tccgttcttt aaattcttcc tgtttcccat
    45001 cattccattg tcccaccggt cgcagatagc caacaatcct tgaaaaaact tcgcattcag
    45061 atgtttggcc tgtttcggcg catttagggc attgtttgtg ttcaccaaac aggtagccat
    45121 gttccgggca aatgctaaat gtgggcgaaa gggtaatata aggtaaatgg aacgtatggg
    45181 ttattttctt aatcagcgct tttacagaat ccacatgcaa ctgtttttcg cccaaaaaag
    45241 tatgaaatac tgttcctccg gtgtatttgg tctgcaatcc gtcctgaata gttaaagctt
    45301 cgaacaaatc gtctgtataa ttaacaggta attgtgttga attggtataa taaggctttg
    45361 ccccttttcg cacatgtact tcgttggcag taacaatgtc gggatattct gctttgtcaa
    45421 tttttgccaa acggtacgat gtgccttcgg ctggtgtggc ttctaagttg tagatgttgc
    45481 cagtttcgtc ctggaatgtt tcgagttttg aacgcattac gtccattact ttttgggcaa
    45541 aggcatagcc tttttcctgt accagattgc aacctaagaa gttgaggcaa cattcgttca
    45601 tgccaacaat accaatggtt gaaaaatggt ttttccaaaa cttacctgta cgttgtttta
    45661 catggcgtaa ataaaatttt gaatacgggt aaagcccttt ttcggtaagg ttttcaatta
    45721 ctttgcgttt tacttcaagg ctttctttgg ccaattccat cagcttatcg aggcgttgga
    45781 aaaattcggt ttctgattta gattcgtaac ccaaacgagg caagtttatg gtaactactc
    45841 caacacttcc ggtaagagga tttgcaccaa ataagccacc accacgtttt tgaagttccc
    45901 gtttgtctaa gcgtaagcgg caacacatgc tgcgcacatc gtcgggactc atatcggaat
    45961 ttacgaagtt ggaaaaataa ggaataccgt atttggctga tatttcccat attttggtgt
    46021 aagcagggtt gtcccactcg aaatctttgg taatgttata ggttggtatt gggaatgaaa
    46081 atacactgcc atttgcatca ccttcggcca ttacttcagc aaatgcagca ttgaatatgt
    46141 ccatttctgg ctggaaatca ccgtaggttg cttcttgagg ttttccgcca attacaacgg
    46201 gctgattctt taaaaactca ggtattttaa gatcaagcgt aatattggta aacggtgttt
    46261 gaaaacctac acgggttggc acgtttatat taaacatgaa ttcctgtaag gcctgtttta
    46321 cctgtgcata ttctagctta tcgaagcgaa taaatggcgc aaggtaggtg tcgaagtttg
    46381 aaaaagcttg tgctccggca gcttcgccct gcatggtata aaagaaattt actatttgcc
    46441 ctaaaatggt acgcaagtgt tttgccggac gactttctat ttttcctgtt acgcctttaa
    46501 aacctgtaat aagcaaatct tccaaatccc agccaacaca gtagacactt agaaagccta
    46561 aatcgtggat atgaaagcga cctgattgat aggcttgttt aatttcatcg gaatagatgc
    46621 gttctaacca atattgagag cttacaatgg tggcaatatg ttggtttaga ccttgtaaag
    46681 aataggatga atttgcactt tcttttatgc gccaatcatc gagactaagg tatttttcaa
    46741 cgatgtcaag attggagaaa agctcttttg cattacgaat attttcatgt tgcttgcgat
    46801 gaagtatgta tgctttggca acatcaaaca atttattttc aaacaacacc ttttctacag
    46861 tgtcttgtac ttcttcgaca gaaggcgtga aattcacctt ttcgaacata ctcaattgtt
    46921 tcaacacatc gagcatgaga ttttcggcta attgacggtc gggacggcca acagcacgca
    46981 aggctttaaa aatagcaaaa gtgatttttt cagcttcgaa agggagtata cgcccgtcac
    47041 gtttactgat ttgaacattt tttaatgcac cattaatcac cataggtttg atatttagta
    47101 agttaataaa cctaaattat ggtagctttg aataaaaaag gggaatgaaa aataaagata
    47161 tctgcgctca gaagccttta ctcttcgcct ttcacccgaa agctacgaat tacattacag
    47221 gcaggtcttc tggctcgtcc tattttggct gccttcccat tccggcttaa cggaacagtg
    47281 gcgagggtgt gccaaaactt tttaaaggac ttacagctac ggggatagcc ccggatttaa
    47341 accgggttcc cttttaatcc acaatcccga aagtttcggg acaggaacct gtaaatttta
    47401 acgaaacaaa ggaatgtcat tttttcaact aaacaatagc aaatcgtatt aacttttgaa
    47461 cagatggttg ttgaaaagat gcaaaaagcc ctaaattgta tgagctgcac ccatgaaaat
    47521 taatttattt tacttaatcg ggatttaggt ttggatgaaa gtttctcact taaaagcatc
    47581 atgtcatcac ttacttttct atctaatatt ttagcatagt gctgtgtaat ggttaaattt
    47641 ttatgcccaa gcattttgct tacactttcg attgaaacac cattagttaa agttatcgta
    47701 gttgcaaaag tgtgtcgggc aatgtgatag gttaattctt tctcaatttc gcaaaagtcg
    47761 gcaatttcct ttaggtagct attcattttt tggttactaa gcacaggaag taacttgcca
    47821 gaatttaaac attgtggatg atttttgtac ttttccagta tttctaatgc cattggcaac
    47881 aaaggaatag ttgcacgggt atctgttttc tgacgatggg tatatatcca cttttcacca
    47941 tcaataccca ttgatatttg cgagctgtca agctttttta catcggcgta agctaaacct
    48001 gtgtaacagc taaataaaaa tatgtcccgt acctggttaa gtcgttcgga tttgaacctt
    48061 ttatttgcaa tagcctgtat ttcttcgttg ttgagataag cccgttcaac ctctttaatt
    48121 ttggctttgt agttcctgaa agggttcttt tctaaccaat cattagctat gcaaatacga
    48181 ataaccttgc caaaattctt aatgtatttg actgctgaat tgttgttgca cttgcgaacg
    48241 ctacggagat aaaactcaaa ttctgttata aaagcatggt caacctgcct aatgtcaata
    48301 tcggaaacag aatacttcca ctttaaaaag tcaatggtat gttttaatga agttttgtag
    48361 cgttgtaaag ttcctgaggc atattcagaa cccaccaaag cctcaatttg tttgttgtgg
    48421 ttttcaaata ttttcaccag tgaacggggc ttttcgcctt taccgatgta tttatttttc
    48481 aatgtttcgg ctgtgattaa atttcccgaa tttaatagct gacgatgggc ttcgtaaacc
    48541 tttgtctgaa tggtatcaag gtaggtattc agggctttcg attcttcttt attacccgat
    48601 gcccgacccg ctgacggaat ccatttgtcg ggtatgcact cacgccctgt tgatgcttca
    48661 gctcgttggc tattaacagt aatacgcaaa taaataggaa ctggacccgt ttcgtaggtc
    48721 tttggcttct tcaaatagaa gagcaaacta aaagttgtgt tcatggctgg tgaccttttt
    48781 aactcacttt ttaatgatac aaatttagat tttcagccaa tgctatacaa gatgttcaaa
    48841 ttctgaacca cctattatac aagcgtttga agtagttctg gtgagtaatt tttagccttt
    48901 gttttactca ccgaatgact caccaaagtt gtgataattg gtgaatattt tgatgaagac
    48961 taaaaacgta aaagcctgta agtcatacga tttacaggct tttgattcgt tttggttatg
    49021 tttctgcgga gagttaggga ttcgaaccct agaagccctt ttggagctta cacactttcc
    49081 aggcgtgcgc cttcgaccac tcggccaact ctcctttgat gctgcaaaga aaacatattt
    49141 tttccaatct gccaattttt ggctattgcc tttgagctct tatttagttc gtaatttgtc
    49201 attcgtcatt cgtcattttt actacatttg cataaatagc ataatacatt aaaatatgtg
    49261 gaaagaacat accaaaaaat tttacaggag aatctttgac tactttttgc aagggcttct
    49321 gtatattgct cctttggggc ttacggtgta tttctttgtg ctaattcttc agtttatcgg
    49381 tggttttgcc caggaatatt ttgataaatg gctccctttc catattcctg gcttgggagt
    49441 gatattaatg tttgcactta ttactattgc cgggtatttt gggcataagc tgatttttac
    49501 ccctttcaag ctcttattgg aaaagctgtt gagcaaggcc ccgcttattg agtcggttta
    49561 tacctctatt cgcgacctga tttcggcttt tgtagggaaa gacaaaaagt tcaaccagcc
    49621 ggttttggtg aaagtgaata aaactgctga gctcgagaaa attggttttc ttactgccaa
    49681 tgacctttca gcgcttaaag taaaaggaaa agtagcggtt tactttccac attcgtatgc
    49741 tttttctggc gaacttttta ttgttccgtc tgaacttgtt accccgcttg atattactcc
    49801 tgccgaagca atgaaattta ttgtatctgg tggtgttaca gaaattaacg ataacgaacc
    49861 tgacaaaaaa tgagtgcaaa gacaaaaata agcgttggga ttattgttgt tgcttttttt
    49921 tgtctgggta ttttgttttg gcaatatttc aaacctcaga agatacttct ttcgccattg
    49981 aatgcagttc ctgaaaatac tgcaatagtt ataaaaataa atggcattct tccttttctt
    50041 cagcatttac aagaaaatga cgtttatcgg gccattaaag atgagcgttt taagactgaa
    50101 atagcaatac tatcaaataa actcgatagt attgctcatg atatttcagg aggtttgtcg
    50161 ttgtttagcg attcttcggt ttatattgct attgaaggtt tgtacaacaa taagcctaag
    50221 atacttgtat tgtttaatat ttctgaaaag ataacaatta gtgagataaa aggttacggg
    50281 caagatgttt ttggtaacac ttttcaaaaa attgacactg ctttaatttc aaatgtttct
    50341 gcgttggata cttcatgggg cttttatctg aaaagcggaa ttttagtgtt gggaaataat
    50401 tatcattttt tggaagcttc aaaaaaatgt cttactggcc gacattcctt attttctgat
    50461 acattgttca ggcaaatggc caatattgcc gggaaaaaag ttgccgctaa tttatttgtt
    50521 caacctaaaa ggtgtttgaa ggctttaaag ccatacattg cagaaagcat atataatagc
    50581 ctggaactta tggttgcaga taaaggtttt actgctttag acatcaatat tgacaatagt
    50641 tttatcgctt ttcagggttt ttcaaaagga acggcttcca attctctgtg gcctaaaatt
    50701 cttagggctg aagaacctgt tgaagctgtt gattggagat tgttgccgga aggagtgtat
    50761 aatttttcag ccttctcaat gtcagaccct tacaactata ctatatcgac caatgaattg
    50821 tataaagcca ttggccaatg gtctgaaaat ataagaaagc taaatgatga cttaaataat
    50881 aacttgctga aaagcttcca gcaatgtatt ggttcgcaag ttatttcgat gactgtaaaa
    50941 agtccaggcg gtcagttaaa taactattat atagttaaaa ctaaccagga agatgctaca
    51001 gtgaggtttt taaatataat ttgtaaagaa gaaaaagata agcctgttaa atctgatact
    51061 tctgatgtga tattgatccg aaagcttaaa aatcctgata ttataaaagt actttttgat
    51121 aaaaaaggag atgctggcaa atacgagatt gtttgcaaat acaatgattt tcttgttttt
    51181 gccgagtctg agtacgcttt atacagttat ctggatgttt taaaggtaaa caagtcaata
    51241 cttaacgatt ctgtctttgt ttcgggctgc aaaactttat tttcaacgca aagtaattat
    51301 atcacgtact tcaacttaag caaagcttca gaagtctttt ttgaaaacct tactaataaa
    51361 ggaattgatg ttgccagtcg ttatttgggt ctggagacgg gtaaagctac ctggggcttt
    51421 cagctttctg gcaataacga aatgtcttat tgcaatggtt tcatcagaaa tgaagtagat
    51481 gttaaccctg tttcgatgaa gctttgggaa gtgcctgtat ctgctggtgt tgccgaagtg
    51541 tataaaatgt ataatgctga ttcgtccatt tgtcgtataa tagctcagga tagcctcagg
    51601 aatgtttata ttctttcagg aaaaggagat gttttatcta aaataagctg ccatagtaag
    51661 atgttggggc aacctgtttt ttataaaaat aaaagcaaac aggattgtat cgcatttaat
    51721 tcttctgacg agctttattt cgttactttc aacggcatgc ttttagaaaa cagcccgata
    51781 aaactacgaa ataatgcttc tgccggaatt gcaactgctg attttgataa caatggcaat
    51841 taccgatttt acctgcccct tgaaaataaa gatattgtag ctttagacca aaaagggaaa
    51901 aaagttgatg gttgggcttt taaaaagact aatggtgtcg ttcgcctgcc tattttatgt
    51961 ttttcttata aaaataaaga ttatcttgtt gttgctgatg ataataaacc cttatttctt
    52021 atgcgaaatg gccttgaacg tttcaaggtc aataaagctg ccaaatggga aaatatagcg
    52081 gtgatggggg ttgaaaagct tggcaggtac ttcaaattat atacacagga gaaagatggt
    52141 tgtttaaaat ctgtctttcc cgatggccat attgaaaagc ttcactgtga ttcgttcagc
    52201 atgtttttaa acggccaaca aatatttttt gttaagaaag attctttgac aataaaacta
    52261 tataatacca actttaaacc gctaattgac agggcactgt ttaacactgt ttcatcaatt
    52321 acagattata ctttttcaga taatatgggt tttatagcgg cttttgacaa actctcgggc
    52381 ttggtaacac tttgtgacca gcaaaaagtg gttagcggct ttccggtcaa tgccttttct
    52441 ccttttattt tttgtacttt aaaaaaacac aaacctgaca atattattgt tgtctgcgaa
    52501 aataaaaagc ttgctgccta ttctgtgcct taataacaaa acagcttttt caatataaat
    52561 tgttgagctt tcataaaagt atttcatata tttaaaccaa ttttgtatat ttataaataa
    52621 gaataactta gtcagacagg cttaattgtt tataaatgat tgcattagag ttattggtgt
    52681 aaaatcatta aagttagttt cgatgaaaaa ccaaatgtgc atagtgccaa aaatattgtt
    52741 tgcaggcttt gtagccctgt tggcatgggg gtgtgttgat aatgaatacg acattactga
    52801 tatatctatg aaaatggagt atgaccctaa cctggctttt cctattggga gtactactat
    52861 taaactaggc gatttgctcg aaaaatatgc tttgcccgaa aatttgaaag aaggcacaga
    52921 tggtagcttt actgttgagt acgaagaaga gcttgagcct ttgcgcggcg aacaggtatt
    52981 tttagtgcct ttacaaaacc aaattgtagt tcctggtgtt atttttcctg ctacaatacc
    53041 tgcttcaggt tctgttaccc gggaagtaac gtttatattt ccggtcaggt ttcttgaaac
    53101 acaacaggta gatagcctca ttaccaaatg tttttatgcc aatatttggg gacagtcaac
    53161 ttttgatccg gctttgtccc ttcaagctaa gattgcattt ttagatatga aaaaagatac
    53221 agttattgga ggcagcaaaa cctccctgac atatactgaa gaatttagcc caacagctaa
    53281 ttataactac ttaacaccta ataaatacaa agcatacaat tataagctca ggttttcttc
    53341 cactaaagac agtgtttctg aaacatctgt aaatgtttcc ttgacattgt caggtgctgc
    53401 aggtacccct attaacccgg caagttattt tacagctatt atgcagctca atgagctcag
    53461 ataccgcgtt atgtatggtt atcttggaaa gaaaactatt cttgacatgg tatatacttt
    53521 taatgtcgat tttctgaata gagaaatggc ccggaatatt aattggtata atccgcaaat
    53581 agggattaaa attagaaata gttatgctgt ccctgttcag ttttccattg ataaaattga
    53641 aatgttctct tcggttcaaa accaattgat aaatgttagc cctgatgcaa cggccaacaa
    53701 tcccaggttt ataaattcgc caaaagctgc ttttcaggaa gtgagtgata atatcagttt
    53761 tactaaatac aatggttata aaagttttta tgaagggata gaaaatagtg cccccaaatc
    53821 tattatttat catgtgtcgg ctgttgccaa tcctgatgca gtaattgata aacacaatat
    53881 tgctatggat acaagcgcta tttttcctaa gttttacttt aaactgccta tttggttccg
    53941 ctcaggcggc tttggatata ccgatacgct tgattttgat ttggccggga tgcaaaaaga
    54001 taaccaggat gtagaactga aatctttgtt gatccgattg gtttctatta acaatatgcc
    54061 tattgatatg aatttgcagg cttatttttt ggatgagcat aacaatattc ttgactctat
    54121 gtttaatact gccgggcaaa gcaggcaggt acttaaagcc ggaactgttg accctgcaac
    54181 aagcctggta acagcgccaa ctaaaactgt aaaagatatt tcgtttactc cttcgcagtt
    54241 aagtaagatg aaaaatatga aaaagttgat atataaggtt tctgttgcta ctgctttata
    54301 cgatacacag cctaaccttt atgtcaaatt tcagaagcaa aacagcctga aaatatcttt
    54361 tgcctgtcag gtacaaccaa agataaccag agaataagta gaaccaataa acctaaagta
    54421 tatgcgccaa gctattatag ccatagtaat attgttcaca gggataaatg ttaaatctca
    54481 ggagcccaat acattgtact ttatggacag ggtgccgcag gcaagcatga tgaaccctgc
    54541 ttttgtgcct aaatgcaact tttattttgg tatccctgct tcaaactttc ataacaatat
    54601 caattttgct cctttaaagt ttaatattat ttttccttat gacaatgtgg ataaaacggt
    54661 cgggaatttc atgagaaatg ataacactaa ggcttcgttc ctaaatacat taaattcaaa
    54721 caatgagcta tatactgatt ttcaggatga tatcttaagt tttgggttta gaattaaaga
    54781 ttggtatttt catttcaatg cttctgcccg ctcaactttt agttttgctt atcctaagac
    54841 atttgtagag tcattgctaa tcaatggttt tgaactgaat accatgtatg attactcaaa
    54901 actggggctt tcaatgaatt cttatgttga atttggtttt gggctgacaa ggcaaattaa
    54961 tgaatcttgg acagtagggg ctaagatgaa ggtgttgcgg ggtatagcta atgtcaggac
    55021 aaataataac caattttcaa taggtacagt tgatgaaaat gatttgtatg ttttgcgcgt
    55081 aaagtcagat ttttctgttg atatggccat tccttattac aaggtaaaca aaggagtgaa
    55141 cctttcatta gacagcctta ttgaaaaaga taacgattat aagattaaac cttttgagaa
    55201 tatcggtttt gcatgggatt tcggagctac ttattcgggt attgaaaatc ttgtgctttc
    55261 tgcttctgtc attgatttgg gttctatctc ctggaaagat aatatcagca acttaaagct
    55321 gtcgtcaaat tttgaatttg atgggactga ttttgtggct gatagttttg acaatgcttt
    55381 tgatgattct tttgaagcgt tgaaagattc tttcaaattt accagctcaa gcaatgtgtc
    55441 atattctaca gctcttccta ctaaaatata tgttggaggg gaatattctt tggctaggtt
    55501 tttctcgatt ggggtactct cttccacaag gatttatcag gctgaatatt ctgaacaact
    55561 tacgttttct gctaatctga aacctgcaaa aatggctatg ttgtctttaa gttattcaat
    55621 tattgataat gctgctcata cctttggtat gggtttttct tttcgtatca taccgcctac
    55681 ccagctttat tttgtttggg acaacatccc cgtttattat gggaaaaatt ttttcccgta
    55741 taaaatggat agaatgaata ttaggcttgg gatgaactgg gtttttggct gcaatggccg
    55801 gacaaagaaa ctcaaagaca aaccaatggg ctgggagtaa agattaagat atagaaacag
    55861 cgtctatact caagtcaagg caattatgta acttcacagt taccgttgtc tctatatttt
    55921 gctccgattc aaattctatt tgagcattat ggatagttgc tacttttcgc acaatagata
    55981 atccaatgcc gctaccttga aggtctcctg catttttcga cctgtaaagt ggcttgaata
    56041 ttaactcaat atcgttagcc ggaattccaa tgcctttatc tataactttt acaatggcca
    56101 gattgttttc atgccctatt ttaactaatg ctttgtggtt tgacgaaaat ttgcaggcat
    56161 tgtctataag gttcgtaaac attactttga gcagccggac gttaccgtta atgtaaatag
    56221 cgttttcgtc ttcgggtatg tt
//
LOCUS       DAOSPF010000014        55239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_629_length_55239_cov_7.163906, whole genome shotgun sequence.
ACCESSION   DAOSPF010000014 DAOSPF010000000
VERSION     DAOSPF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 55239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_55239_cov_7.163906"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..532)
                     /gene="cas2"
                     /locus_tag="PK252_04445"
     CDS             complement(257..532)
                     /gene="cas2"
                     /locus_tag="PK252_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOT13992.1"
                     /translation="MLVVSYDIADDKLRRKFSKMLEKNGGVRLQYSVFEINNTKRMQD
                     ILRVKIESVFAEKFGGGDSIFIFETELDHAVKYGNAIHRDQPLVYFP"
     gene            complement(536..1507)
                     /gene="cas1"
                     /locus_tag="PK252_04450"
     CDS             complement(536..1507)
                     /gene="cas1"
                     /locus_tag="PK252_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HOT13993.1"
                     /translation="MFTHKDIEYRSVFVINCIEERALRVMNGELLLEDTEANKTLTKL
                     PFQKILALFVVGHITITTPLIDKCTRFGIPVVVMKPNFRPVFFFSVTAEANFLLRQKQ
                     YEWDKEDISIPKILVANKIQNQLRLLEKTRKKTEQIDKAKEHCAKAISLLKEAPDYDT
                     VMGIEGRIAKSYFAAYFDELGWKARCPRAKTDPVNVTLDIGYTILFNYVEAFVRLFGF
                     DPYIGVYHRLWFRRKSLICDLMEPFRCIIDQQVRKAFNTKQCKDEDFLLFKNEYVLKR
                     EKNSDYTHMFYDVLIEYKSDVFRYVRSYYRCFMQRKSVPDYPTFNIN"
     gene            complement(1494..2060)
                     /gene="cas4"
                     /locus_tag="PK252_04455"
     CDS             complement(1494..2060)
                     /gene="cas4"
                     /locus_tag="PK252_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HOT13994.1"
                     /translation="MQDYIAISTLNDFIFCPYSIYLHNVYMGGDEDLVHATPQTQGKA
                     AHEAIDEKKYSTRKDEITALSVYSNELGIIGKIDLYKGNEKALIERKYLLHTIYQGQI
                     YQLWAQYYCMTEMGYEVKHLSFYSISTNTKYPIDVPDENGKQELVDFITRFKQFDPTK
                     PIHTNINKCAHCIYCNLCDKTETENVYS"
     gene            complement(2066..2248)
                     /locus_tag="PK252_04460"
     CDS             complement(2066..2248)
                     /locus_tag="PK252_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13995.1"
                     /translation="MASIVRGFKIGVTKYARERGMAFEWQARFHDHIVRNDDEYWRIR
                     RYIQNNVQHWDVDSLK"
     gene            2369..2785
                     /locus_tag="PK252_04465"
     CDS             2369..2785
                     /locus_tag="PK252_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT13996.1"
                     /translation="MRPYGCDHIIVRRRFNDIANVFKTFVTTDPFDGKQQMHVVGHNN
                     KFVHRYKRKMQGYIHGTLVCLPAQVGHFHCLHPITIGNNMAKTMHPAMRANGNKIRPV
                     GGIIPTGATCRGAAVFVLVFIGRHRMSGLLGFNVWL"
     gene            complement(2832..6665)
                     /gene="cas12a"
                     /locus_tag="PK252_04470"
     CDS             complement(2832..6665)
                     /gene="cas12a"
                     /locus_tag="PK252_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HOT13997.1"
                     /translation="MKKDLSEFTGLYSLSKTLRFELVPQGKTLEHIEKNGFLIQDEHR
                     AESYQEVKKIIDDYHKAFIEKALTGFKLKYASENKNDSLEEYYSLYMQKQRDDNLKKK
                     FEDVQAKLRKQIAERFEKDSAFKDQFKRLFAKELIKEDLKTFVKDEKDWQLVSEFENF
                     TTYFTGFHENRKNMYSAEEKSTAIAYRLVHDNLPKFIDNINSFEKAKTTAVAENLPQL
                     YKDLEAYLNVKTIDDIFTLSAYSDFLTQTQIDVYNAVIGGKTLDNGTKIKGLNEYINL
                     YNQQQTDKTKRLPKLKPLYKQILSDRNAISWLPEDFKTDKEVLESIEKCYREIHEHVF
                     YKQKEGEHSLIELLQLLKDFDLHKIYLRNDTGLTDISQKMFGHWGVIQKAIEKRYEND
                     FPQKNKESQEKYQERKEKYFKDFDSISIGFINECLALLGEGYDKKVDGYFAALGKEEK
                     ENKEDKETKSLFEIIADNYKKAEDLLKISYQGSSLAQDPVAVEKLKLLLDSIKAVQWF
                     VKPLLGKGNEADKDEKFYGEFTELWKTLDQITPLYNKVRNYMTKKPYSEEKIKLNFEN
                     STLLDGWDVNKEADNTSVILRKDGLYYLAIMDKKFNTIFKGKLPDDGTCFEKLVYKQY
                     KAEMDIQNLIITENGVKRFTKNLDELKKKYIPEIFEIKKKQSYVQDKPKAEVKVFDER
                     DLGKYIEYYKSAVSNYLGIPFNFRDKYNTWQDFTTHVTEEAYKLYFINVSENYINQLV
                     EESKLYLFKIYNKDFSPHSKGTPNMHTLYWKMLFDPENLKNVVYKLNGQAEVFFRKSS
                     INAEHTTIHPAKQALDNKNELNQKKQSIFEYDIVKDKRYTIDKFQFHVPITMNFKGIG
                     LNNINARVNQFIKDNSDDIHIIGIDRGERHLLYLTLINPKGEIVKQFSLNEIVNEYKG
                     NTYRTNYHDLLDKREKARLEERESWKTIETIKELKEGYISQVVHKIAELMVQYNAIVV
                     LEDLNLGFIRGRQKVEKSVYQKFEKMLIDKLNYLVDKKKAPTEVGGTLNALQLTNKFE
                     SFKTMGKQSGFLFYVPAWNTSKMDPVTGFVNLFDTRYDNVKSAQAFFSKFDTIRYNAE
                     KGYFEFVVNDYTRFSGKAEGTRQNWVICTYGSRIVTFRNPDKNSQWDSKEVQLTQEFI
                     NFFNHYNIDYKSSGLKEAIVSRTDKTFFYNDGKEHKNEPEGLLQLFRLTLQMRNSITG
                     TDVDYLISPVADNNGVFFDSRHAGDKLPKDADANGAYNIARKGMWVVEQIKQAPDIKK
                     VKLAISNKEWLQYIQ"
     gene            complement(6916..7236)
                     /locus_tag="PK252_04475"
     CDS             complement(6916..7236)
                     /locus_tag="PK252_04475"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOT13998.1"
                     /translation="MERRHLTHFNIAGFTYYDGPIVFNELKIGTELRLVAEPDNRYDP
                     KAVALYFGEHKLGFVPRGENSPISKLLEQGYAEIFETRIQRINATDHPENQVGVVVYL
                     KKNG"
     gene            complement(7281..8195)
                     /locus_tag="PK252_04480"
     CDS             complement(7281..8195)
                     /locus_tag="PK252_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT13999.1"
                     /translation="MDQPRIERALRLLPLLIGNRRNTNELAEILDTKVRNVQRYIDTF
                     RAAGFIVEYHSRGIPYLNPNDGRMKKLSDLVHFTEEEAYILHKAIDGIDDGSTLKQNL
                     KAKLYNIYHYPWLADVIVKPGQGENVHALIEAIEDKRCAVLKNYRSSNSNTVEDRQVE
                     PYSFTTNYQQVWCFEPASKSSKLFKVSRIGEVEVLANNWQNEKLHQLPQIDVFRISGH
                     DYIGKAELKLNIRAYNLLVEEYPLAEQYVNCQNHNSFIFSAPVCSYEGVGRFILGLYT
                     DIQILGDEKLKAFIHEKINYLIKYNDRN"
     gene            8593..8781
                     /locus_tag="PK252_04485"
     CDS             8593..8781
                     /locus_tag="PK252_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14000.1"
                     /translation="MFEKKTTFKSPDLGELQEVKIDFKTKIYIPLGKNPEEAKRRILE
                     REAEKSDSIAFRKKPVTA"
     gene            complement(8862..10004)
                     /locus_tag="PK252_04490"
     CDS             complement(8862..10004)
                     /locus_tag="PK252_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14001.1"
                     /translation="MYYNRLAERLLGEYISQFRVVGVMGPRQSGKSTLVKQLLGDRYV
                     YVNFDNIELRNLYYTDPVAFMRKYNQHVIFDEVQQMPDIFQRIKEIVDNNPEERGQFV
                     LTGSGQFLLSKNISESLAGRIGLISLLPMQYKEGPKSLQETFVINGGFPELLSGSFKQ
                     THNFFNAYVNTYVQKDLRQMLNIVDINAFTLMLKLLAARVGQQLNLSDIAKETGITVS
                     TLSKWVSVLEASFIIFLLKPFYANIGKRLVKTPKVYFYDNGLLAFLTGIKTMEQWQDG
                     VLYGALFENLIISETLKNIYHNGDTANLWYLRTSHGDEIDLIVEDGQKVSCVEIKASV
                     TYKPVFHKTIEKLSLPFIEKNIVYQGDTQEVITGVTAKNYKEYLDF"
     gene            10184..11794
                     /locus_tag="PK252_04495"
     CDS             10184..11794
                     /locus_tag="PK252_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT14002.1"
                     /translation="MITVTNLGIQFGKRVLFQDVNMKFTPGNCYGIIGANGAGKSTFL
                     KMLYGDVENMRGSVTLGPGERLSVLKQNHFEFDECTVINTVLMGHSELWGIMQEKETL
                     YAKADFSEADGIRASELEERFNEIDGWNAENEAASLLSGLGVKEYLHSKLMKDLEGKD
                     KVKVLLAQALFGKPDNLLLDEPTNDLDIDTVTWLENYLSNFENTVLVVSHDRHFLDAI
                     CTHTVDIDYNKVQLFAGNYSFWYESSQLALRQQLAQNKKAEEKKKELQEFIQRFSANV
                     AKSKQTTSRKKMIEKLNIEEIKPSTRKYPGIIFTPGREPGNSILEVKGLTKSIDGTVL
                     FKDLTFTVNKNDKITFLSKDTRAITALFEILKGHDKPDSGTFQWGQTIVKAYLPLDNA
                     KFFEKDINLIDWLAQFSEDTSELYLRGYLGKMLFSGEEIYKKANVLSGGEKVRCMIAR
                     MMIRNANVMLLDSPTNHLDLESIQAFNNALTKFKGNILMTSHDHEFIQTVCNRFIEFS
                     PKGMIDKEMDFDDYLSDERIKELREKLH"
     gene            complement(12367..13002)
                     /locus_tag="PK252_04500"
     CDS             complement(12367..13002)
                     /locus_tag="PK252_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOT14003.1"
                     /translation="MHDLSLFLTTVFLGFFAILNPIGNTPVFISMVGESEDKVIKKFA
                     FRAVFVAFIIITTFCLFGHVIFRMFGITLPAFQIAGGIIVFFIGYDLLQGKAAGAHQT
                     KMENDTAKLNDMAISPLGIPLLAGPGTISSAMNFVGEGNSFLITLLVILVFAVVCTIT
                     FFMFIISKKIADKLSPSLIKVVSRIMGLILTVIAVQMFINGVFKVIKEFGV"
     gene            complement(13062..13631)
                     /locus_tag="PK252_04505"
     CDS             complement(13062..13631)
                     /locus_tag="PK252_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HOT14004.1"
                     /translation="MLNTQRKLRKIEDEIWKEVPGSNGRYFLSNYGRLKSFVYKKEGA
                     ILKGGETNGFKLVEIQFKGEKKQRLYLHKLLAEVWLTKPSEEHTFVTHLDGNLKNNHI
                     SNLVWMTKEQLDEHHRQLKAKIPPEARVRQITYSKLKVRDVIAIKKMLQRGVQNVDIA
                     KMFCISEMQVTRIKRGENWGHVKVELNSK"
     gene            complement(13749..13838)
                     /locus_tag="PK252_04510"
     tRNA            complement(13749..13838)
                     /locus_tag="PK252_04510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13802..13804),aa:Ser,seq:tga)
     gene            complement(14083..16254)
                     /locus_tag="PK252_04515"
     CDS             complement(14083..16254)
                     /locus_tag="PK252_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT14005.1"
                     /translation="MKRLILLQLAFLLSFYFVNAQEADSIIEKKIDVLIKQMTLDEKV
                     RMCHANSKFSSRGVPRLGIPDLKMSDGPHGVRAEISWATWDYAKWTNDSVIAFPALTC
                     LAATFNPQMAYEYGVALGEEARFRNKDIILAPGVNIYRHPLNGRNFEYMGEDPLLAAQ
                     MAVPYIKGVQSIGVAACIKHFALNNQERWRGHINVKVSDRALYEIYLPAFKAACQEGD
                     AWTLMGAYNKYNSQHCSHNDFLLNKVLKKDWGYKGVVMSDWNATHNTLEAANNGLDIE
                     MGTWGKRWDTIFDFSEYYLAKPFEQALLKGEVSEDVLNDKVRRILRLCLKTNMRTDRP
                     IGKIGGEHSIIARNIAREGIVLLKNNNQMLPLDASKTIKIAVIGENAVKPMTPGGGSS
                     ELKALYEVSPLDGIQKRFKNAAITYSRGYESPTFAWGKKPELKFNADSLAKAAIEAAS
                     KADLVIFVGGLNKNFFQDSEGGDRKSMDLPYNQDSLINEILKVNPNMLMVLLTGNAVS
                     MPWINKVPAIVQAWYIGSEAGNVIADVISGDVNPSGKLPFTFPVKLEDCPAIRNGAIS
                     YPGDSINQEYKEGIYVGYRWYLTQKIKPLFPFGYGLSYTTFDIKPATSGIQKVKANET
                     FTIKANITNTGKVKGSEVLQLYVRKPKSVVDRPLRELRAFKKVALEPGQTSTVELSVA
                     AQNLSFYDEKKGSWTLEPGEYLIEVGTSAENILFTNKISVE"
     gene            complement(16588..17319)
                     /gene="deoC"
                     /locus_tag="PK252_04520"
     CDS             complement(16588..17319)
                     /gene="deoC"
                     /locus_tag="PK252_04520"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT14006.1"
                     /translation="MKTSITQLAKMIDHSILHPMLTDADLKKECAVAVKYNVASVCVK
                     PYMVPMAKELLKGSDVLVGCVIGFPAGNSTIASKVFETEEACKNGAVEVDMVINIGKA
                     LQGDWDYVEKEIDAVTKTCHKHGAIVKVIFETDFVTKEADIIKLCQICTKVKADFVKT
                     STGYGFVKGADGKYDYQGATISNIKLMRHHCGAEVKVKAAGGVRTLEQLIAVREAGCD
                     RCGASATAAIMEEAEKRRLRKIEED"
     gene            17357..17764
                     /gene="crcB"
                     /locus_tag="PK252_04525"
     CDS             17357..17764
                     /gene="crcB"
                     /locus_tag="PK252_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOT14007.1"
                     /translation="MPYKRCNVVFMIKILLIIGTGSFIGGIMRFLASRYIQNAIISAF
                     PWGTFLVNVVGCFLIGLFYGLSEKNNIASTELRMFLTVGFCGGFTTFSTFANENLALL
                     RNGEFFYFALYAGLSVFIGLLFTYMGNMTTKLF"
     gene            17767..18135
                     /locus_tag="PK252_04530"
     CDS             17767..18135
                     /locus_tag="PK252_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOT14008.1"
                     /translation="MNTAGEAKVLRIYISNTDKFKHAPLYEMIVFAAKRYGLAGATVT
                     KGIMGFGSSSAIHYIKFWEFTEKIPVVVEIIDEAKKIDSFIKTITPWFELISYGCLIT
                     VEKANIVLYKTGKRKKISII"
     gene            18139..18609
                     /locus_tag="PK252_04535"
     CDS             18139..18609
                     /locus_tag="PK252_04535"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOT14009.1"
                     /translation="MEKNKTIGLVAHDNMKHNLIEWVEYNWETLKNHRLVCTGTTGRL
                     VEEAITKKMEHSKSGVENIHILKLKSGPLGGDQQLGAMISEGNIDILIFFWDPMLPQP
                     HEVDVKALLRICVVYNVPSASNRATADFLISSPLFNGYYSPKRIDYSSYTNRKI"
     gene            18937..20340
                     /locus_tag="PK252_04540"
     CDS             18937..20340
                     /locus_tag="PK252_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOT14010.1"
                     /translation="MKFKIEVVTRQILADTITPVSVYLKLRDVFPYTILLESSDYHGS
                     KGSWSFICCKPIATFKVEKGKIYERFPNQTSSVSHINDEFSVADRLNAFRNAFDCQSK
                     QDVPINGIFGYAAYDAIEYFENIKLNTSISEDREIPEIFYSFYQYIIAINQFHNKLYI
                     IENLMQGEKDSTDRIEGLLQNRSYAMYPFKSANNETSNKTDEEYMNMVTLGKQSCQRG
                     DVFQIVLSRRFTQTFTGDEFNVYRALRSINPSPYLFYFDYGSFKIFGSSPEAEIEIKG
                     GKAAIHPIAGTFKRTGNDEQDKILAEKLSQDKKENAEHVMLVDLARNDLSRNASNVTV
                     ETYREIQYYSHVLHMVSVVSGQLPDNYNPVQILADSFPAGTLSGAPKYKAMTLIDKYE
                     GDRRGYYGGCIGFLGFNGDCNHAIMIRSFLSKDNRLFYQAGAGIVLESKEESELAEVN
                     NKLAALKSAIVLAENLK"
     gene            20350..20919
                     /locus_tag="PK252_04545"
     CDS             20350..20919
                     /locus_tag="PK252_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOT14011.1"
                     /translation="MKIIVLDNYDSFTYNLVHSIEKFANSKIDVVRNDKVDVEYFAKY
                     DKIVLSPGPGIPDEAGILKPVIKRWAAEKSILGVCLGLQAIGEVFGGTLENLNKVYHG
                     VASTIIQTVPDETIFHGIPEIFEAGRYHSWIVNRNTLPEELEITAIDKQGQIMALRHK
                     KHDVRGVQFHPESVLTPMGDKMIENWLNS"
     gene            21075..22067
                     /gene="trpD"
                     /locus_tag="PK252_04550"
     CDS             21075..22067
                     /gene="trpD"
                     /locus_tag="PK252_04550"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT14012.1"
                     /translation="MKNTLNYLYSHKTLTREEAKTVLTNIAQGQYNHSQIASFLSVYM
                     MRNITVEELVGFRDALLELCLAVPMDEYNTIDLCGTGGDGKNTFNISTLSSFVVAGAG
                     YKVTKHGNIGVSSGCGSSNIMESLGYKFSSDHDKLRREIEKTGICYLHAPLFNPAMKN
                     VAPVRKDLAVRTFFNMLGPMINPSFPKNQIVGVFSLELARIYTYIYQQSGINFYILHS
                     LDGYDEISLTSDFKVIGNEKDQILSPESLNLPRINPEDITSDKSVEESVKLFLNIIEG
                     KGTPAQNNVVAVNSGLAIQCLNKNKKLEDCIELAREVLISGKAKQVLKHLIEMQ"
     gene            22183..22980
                     /gene="trpC"
                     /locus_tag="PK252_04555"
     CDS             22183..22980
                     /gene="trpC"
                     /locus_tag="PK252_04555"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT14013.1"
                     /translation="MTILDKIIETKKQEVAENKAKTPVSKLERSQYFERKCISFRKNI
                     EMQGTSGIIAEYKRQSPSKGIINNNVPVKEVAEGYFKAGASAMSILTDNQYFGGCNND
                     LLEGRSAVGLPIIRKEFIIDEYQIVEAKSIGADAILLIAACLTALEIKTLTDFAHSLG
                     LEILLELHDESELKKVYDHADMIGINNRNLKNFVVDVEHAIKMAHSLPKKMTKIAESG
                     LDSPHTVAMLRKEGFHGFLMGEHFMKTSNPGLACQNFITELRNIEKR"
     gene            22983..23615
                     /locus_tag="PK252_04560"
     CDS             22983..23615
                     /locus_tag="PK252_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOT14014.1"
                     /translation="MQKKLKIKVCGLRDEKDILKLSLMDIDFMGFIFYKPSPRYAGEM
                     LKPERIENIPERIIKTGVFVNEKAEDTIRIAQKYHLQAIQLHGNESTDLCNELKEKGY
                     IVIKALKGNKEDLVTSSILYKEVCDYYLIDTPTVQHGGSGQKFNWSILNEFEFNKPFF
                     LSGGIGPHDTDIINSLNFRDLYAVDINSKFETSPGKKDTTIISEFIKKIK"
     gene            23622..24833
                     /gene="trpB"
                     /locus_tag="PK252_04565"
     CDS             23622..24833
                     /gene="trpB"
                     /locus_tag="PK252_04565"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT14015.1"
                     /translation="MTDKGNKDYMADEKGFYGQFGGAYIPEMLYHNVEQLRQQYKPII
                     ESASFQQEFKKLLKDYVGRPSPLYYAQRLSEIYKTNVYLKREDLNHTGSHKVNNTIGQ
                     ILLAKAMGKKRIIAETGAGQHGVATATVCALMGLECVVHMGEIDVKRQMPNVLRMKML
                     GAQVIPSMSGSRTLKDAGNEALRDWINNPEDTFYLIGSVVGPHPYPDMVARFQSVISE
                     EIKWQLKEKTNSELPNYVIACIGGGSNAMGAFYNFLNDTSVKLIAVEAAGKGVNTDET
                     AASIAKGSVGIIQGAKTYLMQTNDGQIIEAYSISAGLDYPGIGPQHAHLYNIGRTQIM
                     DATDHEALEAAFELTRVEGIIPALESSHALAALKKLKFEKSDVVVVNISGRGDKDMDT
                     YIANMDKYCQK"
     gene            24845..25621
                     /gene="trpA"
                     /locus_tag="PK252_04570"
     CDS             24845..25621
                     /gene="trpA"
                     /locus_tag="PK252_04570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT14016.1"
                     /translation="MNNIAITFENKKNNVLSIYFTAGYPKLTDTIKILEILQDAGADM
                     VEIGMPFSDPLADGPVIQNSSTVALKNGMSIQELFDQLHGMRTKITMPVILMGYYNPV
                     FKYGVENFCKSAAKVGIDGLIIPDLPIDEYLANKPIFDKYGLCNILLITPQTTDERIK
                     QIDNIASGFIYAVSSYATTGSGKALNLSAQYFEKLKNTAFNNPVMIGFGIKDKATFDN
                     ACQYANGAIIGTEFIKLLEKYGADKTKITEFIKGVKGNDQ"
     gene            complement(25824..25961)
                     /locus_tag="PK252_04575"
     CDS             complement(25824..25961)
                     /locus_tag="PK252_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14017.1"
                     /translation="MKKVALILAVVCMFGFACSSPKKTVSKEKQEQIDKDKKDFEKNV
                     E"
     gene            complement(26020..26562)
                     /locus_tag="PK252_04580"
     CDS             complement(26020..26562)
                     /locus_tag="PK252_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14018.1"
                     /translation="MKTKDILKGGMIGLFMLLFSSVFAQESNKDSAVLHAGHALIYEQ
                     AGNYDLAAEEYTVALSFDSENAVLYDKRGVCYTKLEKYRKALADFNTALMLLPDNLDS
                     YNHRGIVFFLLNNYNKALNDFDEVIHADSSYSKAYFNRGLVKMAINDEKGLSEDLKKA
                     ASLNNEKAISFMKEIGLCQN"
     gene            26834..28837
                     /locus_tag="PK252_04585"
     CDS             26834..28837
                     /locus_tag="PK252_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOT14019.1"
                     /translation="MSSDNQHSVQNEQQGIANNNTENTMNTYLPENGLTMEDKMKDIV
                     PQEQETQNQSNGLEIDEFEMEHMSNIPDEMLIPEDMHDDDDEHEEHSAPVTSEIDYSK
                     LSKEELVATAARLFNERAIDQLKPDFDAIKIHFYKIVKTEQEKIKRQFLEQGGTLEDF
                     KVEPDILETRLKAIFTQYKEKKSAYIQHLESEKLHNLNLKKAVIEEIKDLCNSAESVN
                     DTFNQFRELQKKWRSIGPVPQSEVNNLWENYNHHVEKFYDYIKINKELRDLDFKRNHE
                     AKLELCEKAEELLLEPSIVKAFNLLQEYHAKWREIGPVPFEMRAEIWNRFKDITSKIN
                     KKHQEYFDNQKALQEKNLEEKTKLCEEAEALLNIDNKSVKDWEDRSKALLEIQEKWKS
                     IGFAPKKDNNKIYKRFRNACDAFFAKKRDFYKALKEQQNNNLQLKLDLCVQAEALKDS
                     TEWKKTTDDMLQLQKKWKEIGPVPRKMSDVVWKRFRAACDVFFNNKAAHFSTIDNKYA
                     ANLALKETLISEIENYSPVESAEENFEHLKAFQRRWAEIGFVPMQNKEEIQARYKKAI
                     DKLFETLKVEKSKVNMLNYKNKIENLSHSKHSEKKIDKEREKIIMQIKKLENDIVQYE
                     NNIGFFSKSKGAQLLVAEVQKKIDDAKAEMKSLEEKIKMIDSL"
     gene            28848..30476
                     /locus_tag="PK252_04590"
     CDS             28848..30476
                     /locus_tag="PK252_04590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT14020.1"
                     /translation="MQSKQTSSQPHTTKYIFVTGGVTSSLGKGIISASLAKLLQARGY
                     NVTIQKLDPYINVDPGTLNPYEHGECFVTIDGAETDLDLGHYERFLGTPTTQANNVTT
                     GRIYQTVITKERKGDYLGKTVQVVPHITDEIKRRIKLLGSKHQYDVVITEIGGTVGDI
                     ESLPYVEAVRQLRWELGANNSLVIHLTLVPYLAASGELKTKPTQHSVKMLLENGIQPD
                     ILVLRTEHELSPDIRKKVALFCNVEMDAVVQSIDVSTIYEVPLMMQKEKLDLTVLRKL
                     AMKEGGAPDMAAWNSFLQRFKNPKSTVNIALVGKYVELKDSYKSIIEALYHAGAYNDC
                     KVNLDCIHSEKITEQTIDNLLQGKQGIVVAPGFGDRGIEGKILAVKYAREKNIPFLGI
                     CLGMQCAVIEFARNVLGLTDAHSTEMTRSTEHPVIDLMESQKGVTEKGGTMRLGAYPC
                     KLKKGSKAYKAYGESQIMERHRHRFEFNNEYIDSFEKAGMQPVGINPDSNLVEIMEIP
                     SHKWFVGVQFHPEYQSTVLKPHPLFVEFIKFAKE"
     gene            30486..31559
                     /locus_tag="PK252_04595"
     CDS             30486..31559
                     /locus_tag="PK252_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOT14021.1"
                     /translation="MYSHQYLKDFTASVFKKMGCSPDHAQIIADVFIAAELRGLPSHG
                     MIRLKDYFQLWKANRINVNPNVKIIHESPSTAVVDGDNAIGMVSAHRSMQVAIEKARQ
                     AGTGWVATQNSNHYGIAGYYSMMALEHDMIGICVTNANPLVAPTFSTQRMLGTNPLAV
                     AIPAGKYPAFVADFATTPIARGKLAVLEKKGEKSPLGYVQDKDGKPSDDPSILSKGGS
                     MLTLGGDYEHGSHKGFCMASIIDIFSSVLSGANFGPFVPPSVAYLPVLEEKVGVGTGH
                     FFGAMRIDAFQPADAFKAKMDKWIETFKNAKPAVGTNGVIIPGEPERKKEEELKVKGI
                     SIVPAIEKDVQEIADYFGLKFGE"
     gene            complement(31751..33031)
                     /gene="glyA"
                     /locus_tag="PK252_04600"
     CDS             complement(31751..33031)
                     /gene="glyA"
                     /locus_tag="PK252_04600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT14022.1"
                     /translation="MEKDKLIFDLIEKEHQRQLHGIELIASENFVSDQVMQAMGTWLT
                     NKYAEGYIGKRYYGGCQVIDEVEGTAIERAKKLFNAEWVNVQPHSGAQANAAVMLAVL
                     KPGDTFLGLNLSHGGHLSHGSPVNSSGILYKPVEYSLNPETERVDYDQMAKLAREHKP
                     KLIIAGASAYSRDWDFKRMREIADEVGALLMADIAHPAGIIAKGYLTHPFPHCHIVTT
                     TTHKTLRGPRGGMIMIGKDFPNPWGLTTPKGEIKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVAFGEALTDSYDAYVKQMLANAKVMAKSFTDLGYKVVSGGTDNHSMLIDLRTKLS
                     DVNGKQAENALVAADITLNKNMVPFDTRSPFQTSGFRVGTPALTTRGVKEDVIPQIVE
                     MIDRVLTNIDNQSVIAEVRQKVNKLMSNYPLFAY"
     gene            33238..35661
                     /locus_tag="PK252_04605"
     CDS             33238..35661
                     /locus_tag="PK252_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT14023.1"
                     /translation="MTIKKYFFLLVLLTGLQQLIAQENGILKGIVSDTSGYTVGFANI
                     TLEGTKFGTMADRFGQFETKVPAGKYSLVITCVGYQPQKTEIEIIANQVTTINFTLKE
                     STQKLDEVTVAGRSEKSGTVQRIDAKAFNAVPNTTGNIENVVKQMAGVASNNELSSQY
                     SVRGGSFDENLVYVNDIEIFRPVLIRSGQQEGLSFINPDLVSSIKFSSGGFDASYGDK
                     ISSVLDVTYKKPTAFGASASISMLGATSHFEGSAAANKLRYLAGFRYKTTKYFLGGLD
                     TKGDYEPNFLDFQTNIGFDITPTLEFSVLGNISQNQYDFKPDTREIEFGTYNLPLRAN
                     IYYEGQEKDKYLNGMAAGTLNFHPSDDMYFKLIGSVYQNDESETFDLLGEYNISEIDN
                     SSGQDSSLEIGVGGFLNHARNFLKINIYNVSHIGMVQLDKNKFKWGLTYQSESVNDRL
                     NEWEIIDSAGYIYPYNIDNIKTLTSVNSKNDITSHRLMGYLQHTFEFESGNDKFFLSA
                     GVRTQYWSFSKQQTWSPRISLSYQPGWKNGLMMYMAWGFYHQHAFFKEMRNEQGLLNK
                     EIKAQQSVNYSAGLDYMFYSWARPFKFSAEVYYKQLDKLIPYRIDNVRSIYSALNIAK
                     GYATGIDLRLNGELVKGTESWVSVSLLQTQEKILANSTIPSSNYYSRPTDQFVNINFY
                     FQDYVPSYPSYRVHLNVHYGSELPVTIPWQKRWDKVQKLLPSYKRVDMGVSKMLKGEE
                     VPAKYPWLNVFKEMWLTAEIFNIIGLNNTASFMWIKTFSNDPDIPSSFAVPNYLPGRR
                     FNIKLTATF"
     gene            35695..36156
                     /locus_tag="PK252_04610"
     CDS             35695..36156
                     /locus_tag="PK252_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOT14024.1"
                     /translation="MIQRIQTFYLIAAIICLFVNTLVPMAYFTDHANNTFYLMHNGLQ
                     ELVKGKIIQVYYSLSITILLSVAIAMAVYIIINFKRRALQMFSCKILWVAIFAIQGSI
                     LWIYFMIKPQFVHGKLSFTAFLPLLAMIFSILAFRNIKKDDDLVKSIDRLR"
     gene            36153..37292
                     /locus_tag="PK252_04615"
     CDS             36153..37292
                     /locus_tag="PK252_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT14025.1"
                     /translation="MKTGFLVFITIALSIYILGNWYLFTRLRQSWPEQWQGFSLVITL
                     FLFCAGCFVLSRFIGHTRYTGLYNIVSWIGYFWLVILLYSVVILAVFDIVRILNWIMP
                     FLPDSTSIQYKNLKTFTLLVTCCTLVLVILTGYFNANNTKIRKISLSSEKINGKPVVI
                     ALASDLHFGNMVCDKKIQKLVDLINSYNPDIVILAGDLMDEAHEYTFKNKVGQPLKHL
                     KASKGVYAIAGNHEYFGGIKGAIQYIKSLGIEFLRDSVVELDDIVIVGRDDIHNLGTS
                     NTSRMPLSQLIVEKHRNKFILVADHQPINLSESAKAKVDLQVSGHTHNGQMWPFIHLV
                     NLAFERAYGLFKIDETNFYITSGFGTWGPPIRLGSNSEIAIITIN"
     gene            complement(37554..37751)
                     /locus_tag="PK252_04620"
     CDS             complement(37554..37751)
                     /locus_tag="PK252_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14026.1"
                     /translation="MLRENYIVDKKGNKIAVQLPIKVYEKLLADSEELEEIKEYRKAK
                     MHKSEPIPFELAFKEIEEAIK"
     gene            complement(37836..38192)
                     /gene="trxA"
                     /locus_tag="PK252_04625"
     CDS             complement(37836..38192)
                     /gene="trxA"
                     /locus_tag="PK252_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT14027.1"
                     /translation="MVSHLNAATFKEKVFNYETNKDWKFEGDKPCMVDFYANWCAPCK
                     MVAPILEELSVEYEGKIDIYKVNTDEERELSSLFGIQSIPSLLFVPKEGTPQMAMGAL
                     PKESFKRAFKEVLKVE"
     gene            38346..39767
                     /locus_tag="PK252_04630"
     CDS             38346..39767
                     /locus_tag="PK252_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT14028.1"
                     /translation="MKHCLDRKIFSVISDIAERENVQCFVIGGYVRDCILKRPCKDID
                     IVVAGSGIRIAELTAKALKHTTKVSVFESFGTAMIRHHDLEIEFVGARKESYRSHSRK
                     PIVEDGTLEEDQLRRDFTINAMAISLNKEDFGTFIDPFGGQSDLQNKIIKTPTDADIT
                     FSDDPLRMMRAIRFATQLDFEIEPATLAAITKNKDRITIVSAERIADELNKIVLSPKP
                     SKGFKLLDETGLLAIIFPEMSKLKGVETIKGKAHKDNFYHTIEVLDKIAVNSNDLWLR
                     WAAILHDIAKPAVKKYIEGQGWTFHGHEFLGQKMIPGIFKRMRLPLNEKMKYVQKIVG
                     LHLRPIVLAQEVVTDSAVRRLLFEAGDDIDDLMMLCEADITSKNEKLQKKYLDNFAIV
                     RHKLKEIEEKDAIRNFQPPIDGTEICDIFGLSPCKTVGDLKNAIKDAILDGIIPNDRK
                     AAYGLLLAEAEKAGLKIINPLNI"
     gene            complement(39905..40111)
                     /locus_tag="PK252_04635"
     CDS             complement(39905..40111)
                     /locus_tag="PK252_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal transport/detoxification protein"
                     /protein_id="HOT14029.1"
                     /translation="MNTIKLKTNIACAGCKSKVAKVLDNDPKIKTWDVDLVSAERWLT
                     AEVEDVDESYVKAAVAMAGYVCKD"
     gene            complement(40204..41631)
                     /gene="dnaA"
                     /locus_tag="PK252_04640"
     CDS             complement(40204..41631)
                     /gene="dnaA"
                     /locus_tag="PK252_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT14030.1"
                     /translation="MEQNSCQTLWSNCLKVIKDNVSENSFKTWFEPIIPVKLEKNILT
                     IQVPSTFFYEYLEEKYIDILKKTLRRELGNDAMLEYNIVMDNDSEENKPYTVKYPGRS
                     ASVAKNKPVNMPLSEEQPVMNPFVLPGIKKLNIDPRLNPNYSFSNFIEGECNRLARSA
                     GSAVADHPGGTAFNPLMIFGDSGLGKTHLVHAIGLATKEKFPEKNVLYVNAHIFQTQF
                     AEATRNNNRNNFLYYYQMIDMLIIDDVHEFCGKEKTQDIFFHIFNHLHQSGKQLILTS
                     DKAPADLQGMEQRLLSRFKWGLAADLKAPDFETRVAILRNKCYMDGIQVSDDVLEYIA
                     ANITSSIRELEGALISTLAQALNHKTISIEVAKEVVDKISRNVKKEITIDYISKVVCN
                     YFNITPDQLSVNTRKREIVLARQVAMYFSKLLTKSSLTTIGTNIGKKDHATVLHAYKT
                     VLNLRDTDKMFKRQLEDIERKLKAV"
     gene            complement(41869..42780)
                     /locus_tag="PK252_04645"
     CDS             complement(41869..42780)
                     /locus_tag="PK252_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT14031.1"
                     /translation="MKKEIKIGLIMFATLGSLIWGINFLKGKNFFSNTKDYIVIYEDV
                     SGLLESNGVYIKGYKVGHVSSISFSDSSFAHLKVVLSIKKTINIPKNTVARIYNLDLI
                     GNKAVELNFSRSNEFCKENDTIIGEVETTIAKQLEPYKVQAYKLLNSMDSVSNAIYKV
                     FDKQTIQNMKETFKNLRVSSEILASSSENIALTLENVRAISANLKDNNKQVNKIVSNF
                     VAISDSIKKIRIEPRLMLLDSMLTESRNLIRDIKGGSGTVSRLLYQDSLYNSLLNVTN
                     NLDSFITNIKENPNRYIHFSVFGKKNK"
     gene            complement(42871..44064)
                     /locus_tag="PK252_04650"
     CDS             complement(42871..44064)
                     /locus_tag="PK252_04650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT14032.1"
                     /translation="MYKNIYQGIVYKFSVVLVVLSCICMSKTYAVDEQTKSGNEKKNS
                     LTKIVIDPGHGGQDPGALGKNHKEKDVVLAVALKVGKLISQRFPDVDVIYTRNTDVFV
                     PLHERAEIANRHNAELFISIHANANKNKDIKGIETYAMGLHKNESNFDVAKKENAAII
                     FEKDYTTRYEGFDPNSAESYIMFSSMQNTYLIQSLDFAGLVQSNSSRYNSALDRGVRQ
                     AGFLVLWKTAMPGVLVEVGFISNPDEERYLSSETGQMEIANGIANAIGEYKKRFEQKT
                     FYVKNNDDSALKTPVYQDNTDSGTVVSVENVDESVNFKIQILTSQKKVSKKDQVFKHC
                     KKLKLNNSIAEYRINSTYKYTIGNTNQYLQAVELASEVKKYYPQAFIVAVKDGQIVPL
                     QSVVK"
     gene            44122..46761
                     /locus_tag="PK252_04655"
     CDS             44122..46761
                     /locus_tag="PK252_04655"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOT14033.1"
                     /translation="MEIRYIKIKLGRFFLFTYKLCFLLLFLAVNTANAQVDTTNSVQP
                     FDNLIKKDSTLNDTTANAAIANDSVKKKGSDLDEPVKYSAKDSMFIDMENKTVHLYGD
                     AKVTYKDIELKAGYISLATEDQIVYATGTIDSSGNMSNYPTFKDGKEEFQSHWMRYNF
                     KTKKGFVYFVKTKQGEGTLIGDSTKRSASGSVSLKGATYSTCDLDHPHFYMKLTKARV
                     VPNDKIVSGPAYLVIADIPFYMLFVPFGYIPNTPDFSSGLLLPTWGEDKAKGFFFRDG
                     GYYFAISDYMDVTLRGEYYTYGSWGASLSTRYKKRYGYTGNLNFTFARNIYGEKGFPD
                     YQTSNDYKFIWSHQQDRKFNPNSTFSSNLNISSSKYDRHNSYDPAEHTRNTKSSSISY
                     SYKWANLPFSMTTSLNHSQSSQTGKVDLTLPQASLTMETLYPLRKEEGAAKPKWYENL
                     SIRYSSKLDNKVYTNDSAFLTDRMWDRMKNGFSHDIPIELVIKVTKSINVTPILKYTG
                     VAYTEYDTYQWNDNTTNPRTRRNGAVDTITHHGLTYAHALYPSVSTGYSPKLFGMYQF
                     RNSRVKALRHVMSPSVSVSYTPDVSDLLPNYYYKYKSDTNRYTETSWYAGNIYGTPTH
                     PPGKPVASVNFALKNNFEMKIAPSANDTSAKNRKISLLDNFDFSGSYDLIADSFNLSN
                     ITMQTGTSLFDSKLKIDIRGIFNPYKTDSTGNRVVNSYILNDWEQPARLTDLSLSTSI
                     SFSSSELKSKKEDGKKTEQTTEEGDDNSIPWNLNIGYSLTYEHINPYKKYSIIHSCTF
                     SGSLTLTKNWKLGIQSGYDFTNKEFNMTSINITRDLHCWQMTFNVIPFGSMQSYSFNI
                     SAKAAILQDLKYSKVNQSWRGRY"
     gene            complement(46826..47578)
                     /locus_tag="PK252_04660"
     CDS             complement(46826..47578)
                     /locus_tag="PK252_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOT14034.1"
                     /translation="MSNIRVLLVEDEEPARELVKSYFKKFSNIEIIGEAADGFNAVKA
                     INQMKPDIVMLDIQLPKLTGFEILELVEFTPVVIFATAFDQFAIKAFEMNAVDYLLKP
                     FSAERFEVALNKAIEKLKNTKQAETEVQGVLNSMDEKEAIIDRIVVKNGTKIKVIPCP
                     SVHYIEAQDDYVMIYADEGKYLKEKTMKYFESHLDGDKFIRIHRSYIVNVEQIIQLEH
                     FTKDSYVVVLKSGAKLKVSDSGYKNLKSKLNF"
     gene            complement(47550..48641)
                     /locus_tag="PK252_04665"
     CDS             complement(47550..48641)
                     /locus_tag="PK252_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT14035.1"
                     /translation="MNHPILESFRKTIAYLITWLVFAVISFFFLYFKHHLSLEGSILN
                     SIMVNLFFAVMGLTIWYAVYYSYPEKIDISNVIINHCISASVIILFLVGSVTLFNKVL
                     LNPYDANFIQQHIKGFVFTCLIFYITAVLVYYNIVYNYYLKQRKENEERLLSIIKQTE
                     LDMLKSQINPHFLFNSLNSISSLTITNPAKAQEMIIKLSDYLRYSIKYNSTQLTTLQA
                     ELDNMLRYLDIEKIRFGSRLNYRTNINEDCYKATLPVMILQPLFENAIKHGVYESVNT
                     ITLEIEAVMNDNYLVIKVRNNYDSEVPSRKGAGIGLKNIKERLRLTYMTEGLMNFSKT
                     DEVFEVQILIPQQIINEPNLYEQHQGVIS"
     gene            complement(48652..49383)
                     /locus_tag="PK252_04670"
     CDS             complement(48652..49383)
                     /locus_tag="PK252_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOT14036.1"
                     /translation="MRHEHHHITPVDAPRSKKLMFGIIVLCAGVLFLFDNIGILPENI
                     KHIFFSWQMLLIAIGLIAVSGHGAVPGLILISIGVFFLLPYIFNVPFNFTRLFWPVIL
                     IFIGIVIIFRRKPDAKRFCDSFREEVTASDLVDDVNVFSGIKRNINSQNFCGGRVTSI
                     FGGAELDLTNAALAPGKSVMELQCIFGGVTLIVPAEWRVRIEVSSILGGFADKRQVAK
                     ISINSDSELIIRGVAIFGGGEIKTR"
     gene            complement(49627..49926)
                     /locus_tag="PK252_04675"
     CDS             complement(49627..49926)
                     /locus_tag="PK252_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOT14037.1"
                     /translation="MKTLANIHPGEILMEEFLIPMNISAYKLSKDIGIPQTRTSAILK
                     GKRGITADTALRLSIYFGNSAKFWLGLQDDFDLEEAKRAKQSELEKIKKIEYNAA"
     gene            complement(49937..50218)
                     /locus_tag="PK252_04680"
     CDS             complement(49937..50218)
                     /locus_tag="PK252_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT14038.1"
                     /translation="MILSFGDKETEQIYNGIRVKQLPLEIQTIGRRKLRMLNNSQTIN
                     DLKIPPSNRLEKLGGKLNDYYSIRINDQWRIIFQWVEGQSENVKIVDYH"
     gene            complement(50455..52962)
                     /locus_tag="PK252_04685"
     CDS             complement(50455..52962)
                     /locus_tag="PK252_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT14039.1"
                     /translation="MKKIYLKILVLGFALVLLSCKEKAIYQDPSQSVEARVNDLLAKM
                     TLEEKIAIISGDTTGFDTPAIERLGIPAIRITDGPLGVRNGQATSFISGVAMGATWDT
                     ALVKEIGIAMGKETKAKGRNYLLGPCVGIHRFPLNGRNFESYSEDPYLTSRMAVNWIL
                     GVQSQKVITSVKHFATNDQEWERNNYDVKVDDRALHETHLAPFEAAVKEADVKSVMAA
                     YNLQNGQHCTENYHLISDILKGQWGFKGFVISDWVSVYSTVNAANAGLDLEMPVGVYF
                     NKDSILKYIQSGQIKESTIDDKVRRILTAAFWAGLFDNNEKADTSVLHGDAHKKLVLK
                     SAQEAITLLKNEGNILPLSVSKVKTIAVIGPNAAYTATGGGGSSFISPYYDISPLQGL
                     KCRLGDSVKIVYSTGDSIPQPKIEPVDGSFLFCSSKNASGLWAEYYGNKELQGKPVLE
                     RLDKQVNFDYGTGSPAPNIKPNFFSCRWTGELKVKETGKYNLQTLSDDGVRLFLNDKK
                     VIENWNNHGPIVDQYETTLEAGKSYKIKLEYFEDGGGAVIQLGLVRINEKAGDPIENA
                     AKVAASADVAIVFAGTNSNLESEGRDRDGLELPGKQEALVKAVAKANKNTIVVVNSGC
                     PINVSSWIKEVKALVNMYFIGQETGNAIASVLMGDVNPSGKLPFTYIANAKQSPAFEG
                     YKKSLKVEYKEGVFVGYKFLEKNNMEPAYPFGFGLSYTSFAYSNLKVKELAKNTYEVS
                     VDVTNTGARSGDEVVQLYLSDKECSVARPVKELKGFSRVSLKAGETKTVSMKVKERDF
                     AFWDVNTNGWKVEPGEFTFMVGSSSKDIKLTSSIMLK"
     gene            53346..54227
                     /gene="sucD"
                     /locus_tag="PK252_04690"
     CDS             53346..54227
                     /gene="sucD"
                     /locus_tag="PK252_04690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOT14040.1"
                     /translation="MGILLNKHSKVIVQGFTGTEGSFHAQQMIEYGTQVVGGVTPGKG
                     GSFYLNRPVFNTVYEAKKATGADTSVIFVPPAFAADAIMESAEEGIALIVAITEGIPT
                     SDMVKVKSYLSDKNSRLIGPNCPGIITPGEAKVGIMPGFIHSPGNIGIVSRSGTLTYE
                     SVFQISQLGLGQSTCVGIGGDPVIGTTTLEVVKLFMKDPQTEGIIIIGEIGGNMETEA
                     AQWIKDNSSKPVVGFIAGQTAPKGRRMGHAGAIIGGINDTAEAKMKIMRECGIYVVDS
                     PANIGRDMFKALKKFTA"
     gene            complement(54217..55011)
                     /locus_tag="PK252_04695"
     CDS             complement(54217..55011)
                     /locus_tag="PK252_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT14041.1"
                     /translation="MNNAFSFVPLSNALYKRLSQLHDKKFRKEYKQFIVSGDKLVREL
                     LVSGLKIDYIIAEAGWLQEIDKRAVQRYTCYAVDEAVLKKLSGQVTPNRVMAVLPLFA
                     HAKAVLPSKNERIIALDCIQDPGNLGTIIRLADWFGIDHIVCSNDCADFYNPKVIQST
                     MGAFLRVKVHYTNLESYLQEARNANVPVYGAFLKGENIYNASLAKQAIIVMGNESKGI
                     SDKIGSYVTQSITIPSFRVGGAGSESLNVAVATAIICSEFARQSML"
BASE COUNT        17322 a        11284 c        10023 g        16610 t
ORIGIN      
        1 acaatagtag aaatttatta ggctttatag ccaagaagat ttgattgaaa taatatttgg
       61 aatctacaat agtagaaatt tattaggctt tatagcctct atgcaaaaat ttttgcttta
      121 caaatgtagc aatttcaata aagaaaaaca tattaagagt attcttttta cccccaccct
      181 tttgatgtac attatttcaa agaactaata ataaaccttt tacattattt tcataaaaaa
      241 taaagaccaa ggtttgttat ggaaaataaa caagcggttg gtcgcgatgt atggcattgc
      301 catattttac agcatggtcg agctccgttt cgaaaataaa gatactgtcg cctcccccaa
      361 acttttcggc aaataccgat tcgattttca ccctcaggat atcctgcata cgcttggtat
      421 tgtttatctc aaacacggaa tactgcaaac gcacgccccc gtttttttcg agcatcttcg
      481 aaaactttcg gcgaagttta tcatcagcta tatcgtaact tactacaagc atggctcaat
      541 tgatattaaa tgttggataa tcgggaacac tcttgcgttg cataaagcag cggtagtacg
      601 aacgcacgta acgaaacaca tctgacttgt attcgataag cacatcgtaa aacatgtggg
      661 tatagtcgct gtttttctcg cgtttcagga cgtattcgtt tttgaaaagc aaaaaatctt
      721 cgtctttaca ttgcttggta ttgaatgcct tacgcacctg ctggtcaata atacaacgga
      781 aaggctccat gaggtcgcaa ataagcgact tgcgcctgaa ccacaagcga tgatatacgc
      841 caatgtaagg gtcaaaacca aaaagccgca cgaaagcctc gacataattg aatagtatgg
      901 tatatccaat gtcgagcgta acatttacag ggtctgtctt tgccctgggg cagcgggctt
      961 tccatcccag ttcgtcaaag tatgcagcaa agtacgattt ggcaatacgc ccctcgatac
     1021 ccataacggt atcatagtcc ggagcttctt ttaacaaaga tattgcttta gcacaatgtt
     1081 cttttgcttt gtctatctgc tcggtcttct tgcgcgtttt ttccaacagc cgcaactggt
     1141 tttgtatttt gttggcaaca agtattttgg gaatgctgat atcttcttta tcccactcgt
     1201 actgcttttg gcgcaacaaa aaattggcct cggctgtaac cgaaaagaaa aacacaggcc
     1261 tgaagttggg tttcattacc actaccggga ttccaaacct ggtgcatttg tcaatcaacg
     1321 gcgtggtaat ggtaatatgc ccgacaacaa acaaggccag tattttctgg aaagggagtt
     1381 tggtcaacgt cttgttggct tcggtgtctt caaggagcaa ttctccgttc attacacgca
     1441 gtgcccgctc ttcaatgcag ttgattacaa agacagagcg atattcgatg tctttatgag
     1501 taaacatttt cggtttcggt tttgtcgcac aaattgcagt atatacaatg agcgcatttg
     1561 tttatattag tatgtatagg ctttgtcgga tcaaattgtt tgaaacgtgt aataaaatca
     1621 accagttcct gtttgccatt ctcatcaggc acatctatcg ggtattttgt attggtcgaa
     1681 atggaataaa acgacaaatg tttcacctcg taacccatct ctgtcataca ataatattgt
     1741 gcccaaagct ggtaaatctg cccctggtat atggtgtgca acaggtattt acgttcgatg
     1801 agcgcttttt cattgccttt gtaaaggtct attttgccta taatacccag ctcgttgcta
     1861 tataccgaca gggcggttat ttcgtctttg cgggtactgt atttcttctc gtcaatagcc
     1921 tcatgcgccg ctttgccttg tgtttgcggt gtggcgtgta ccaaatcctc gtcaccaccc
     1981 atatacacgt tgtgcaggta tatagaatac gggcaaaata tgaaatcgtt cagggtcgat
     2041 atggctatat aatcctgcat tcctgttatt ttaacgaatc aacatcccaa tgttgaacat
     2101 tattttgtat gtaacgcctg atacgccaat attcatcatc attacgcaca atatggtcgt
     2161 gaaaccgcgc ctgccattca aacgccatgc cccgttcgcg tgcatatttg gttaccccta
     2221 ttttgaaccc acgtacaatg gacgccacgt tttgcgattg tggaccaaaa tggttgcacg
     2281 gggcgttgac gggcggcggt gttttttttt gttgggacgc aatattttgc gtccgttcca
     2341 atggttgcgt ttgtagggac gcaaaatttt gcgtccctac ggttgcgacc atatcatcgt
     2401 ccgtcgtcgt tttaacgata tcgcaaacgt ttttaaaacg ttcgttacca ccgacccgtt
     2461 tgatggtaaa caacaaatgc acgtggttgg gcataataac aaattcgtcc accgttacaa
     2521 acggaaaatg caggggtata tccatggcac attggtgtgc ctgcctgccc aggtcggtca
     2581 tttccattgt ttgcacccca ttaccattgg caacaatatg gccaaaacaa tgcaccctgc
     2641 catgcgtgca aatggtaata aaatacgacc cgttggaggc ataatcccaa ccggggcaac
     2701 gtgccgaggc gctgcggtat ttgttctggt atttatcgga aggcatagga tgagcgggtt
     2761 attgggtttt aatgtttggt tataacggcg caaaattttg cgccgttacc ttgataattg
     2821 taccattggt attattggat atattgtagc cattctttat tactaatagc cagttttacc
     2881 ttttttatat caggagcttg cttgatttgc tcaaccaccc acattccttt gcgggctatg
     2941 ttataagccc cgttggcatc ggcatctttg ggcagtttat cgcccgcgtg acggctatca
     3001 aagaaaacac cattattatc agcaacaggc gaaatcaaat agtcaacatc agtacctgta
     3061 atgctgttgc gcatttgtaa ggttagcctg aacaactgca acaagccctc cggttcgttt
     3121 ttatgttcct tgccatcgtt gtaaaaaaac gttttgtcgg tcctgctaac aatggcttct
     3181 ttcaagcccg atgacttgta atcgatgttg taatggttga aaaagttgat aaactcttgt
     3241 gtaagctgca cctctttgct gtcccactgg ctgtttttat cggggttacg gaaggtaaca
     3301 atacggctgc cataggtaca gataacccag ttttgccttg taccttcggc tttgccactg
     3361 aaccgggtat aatcattgac tacaaactcg aagtagccct tctcggcatt atagcgtatg
     3421 gtatcgaatt tggaaaaaaa cgcttgcgca cttttcacat tatcgtaccg ggtatcaaac
     3481 aggttgacaa atcctgttac cggatccatc ttgctggtgt tccatgccgg cacgtagaaa
     3541 agaaaaccgc tttgcttgcc catggttttg aaactctcga atttgttggt caactgaagg
     3601 gcattgagcg taccaccaac ttctgttggt gccttctttt tgtcaactaa gtaattgagc
     3661 ttgtcaatga gcattttttc aaatttttga tagaccgatt tttctacttt ctgcctgcca
     3721 cgtataaagc ccaggttcaa atcttccaat accacaatgg cattgtattg taccatgagt
     3781 tcggctattt tatgcaccac ctggctgatg tagccttcct tcagctcctt aatggtttca
     3841 atggttttcc agctttcgcg ctcttcaagc cttgcttttt cacgtttgtc gagcaaatcg
     3901 tgataattag tccggtatgt gttgcctttg tattcgttta caatctcgtt gagcgaaaac
     3961 tgtttaacaa tctcaccttt ggggtttatc aatgttaaat acaacaaatg gcgctcgcca
     4021 cggtctatgc caataatgtg aatgtcgtcg ctgttgtctt tgataaactg gttgacccgc
     4081 gcattgatat tgttcagccc aatgccttta aagttcatgg ttatgggcac atgaaactgg
     4141 aatttgtcga tcgtgtagcg tttgtcctta acaatatcgt actcaaaaat gctttgcttc
     4201 ttttggttca gctcattttt gttatcgagg gcctgtttgg ctgggtgtat ggtggtatgc
     4261 tcggcattga tgctcgattt gcgaaaaaac acctcggcct gcccattgag cttataaacc
     4321 acgtttttaa gattttcggg gtcgaaaagc attttccaat aaagcgtgtg catgttgggt
     4381 gtgcctttgc tgtgcggcga aaaatctttg ttgtaaatct tgaaaagata cagcttgctt
     4441 tcttctacta attggttgat gtagttttct gatacattaa taaagtataa tttatatgct
     4501 tcttcagtaa catgagttgt aaaatcttgc catgtattgt atttatccct aaaattaaaa
     4561 ggtattccaa gataattact gacagcactt ttgtaatact caatatattt gcctaaatct
     4621 ctttcatcaa atacttttac ctctgctttg ggcttatctt gcacatagct ttgtttcttc
     4681 ttaatttcaa atatctcagg tatatatttt ttctttaatt catccaaatt ttttgtgaac
     4741 cttttgacac cattttcagt aatgataagg ttttgtatat ccatttctgc tttgtactgc
     4801 ttatatacaa gtttttcaaa gcaagtgcca tcatcaggca atttaccttt gaaaatggtg
     4861 ttgaactttt tgtccataat tgccagatag tacagcccgt ctttacgtag gataacgctg
     4921 gtattgtcgg cttccttgtt cacgtcccaa ccatcgagca gggtagagtt ttcgaagttg
     4981 agctttattt tttcttccga atacggcttt ttggtcatgt aattacgtac cttgttgtac
     5041 agcggggtga tttggtcgag cgttttccat agctcagtaa attcgccata aaatttttcg
     5101 tctttatcgg cctcgttgcc cttgcccagc aggggcttta caaaccattg cacggccttg
     5161 atgctgtcga gcaatagttt tagtttttct acggccactg ggtcttgtgc caagctactc
     5221 ccctggtaac ttatctttaa aaggtcctcg gcctttttat agttatctgc aattatctcg
     5281 aacaggcttt tggtttcttt gtcctctttg ttctcttttt cttcttttcc aagagcggca
     5341 aagtaaccat ccactttctt gtcgtaccct tcgccaagca atgcaaggca ctcgtttata
     5401 aaccctatgg aaatactatc gaaatctttg aaatactttt ctttacgttc ctggtatttt
     5461 tcctggcttt ctttgttttt ttgtggaaaa tcgttttcgt accttttctc gatagctttt
     5521 tgtataacgc cccaatggcc aaacatcttt tgcgaaatat cagtaaggcc ggtatcgttg
     5581 cgcaggtata ttttgtgcag gtcaaaatcc tttagcagct gtaaaagttc aattaacgaa
     5641 tgttcgcctt ctttttgttt gtaaaacacg tgttcatgaa tttcacgata acatttttcg
     5701 atactttcga gaacttcttt gtctgtttta aaatcttcgg gcaaccacga aatggcattg
     5761 cggtcgctca aaatttgctt atacagcggc tttaacttag gcaaacgctt tgttttgtcg
     5821 gtttgctgtt ggttgtacag gttgatgtac tcgttaaggc cttttatttt agtaccgttg
     5881 tcaagtgttt tgccaccaat aacagcatta taaacgtcaa tttgcgtttg ggtaagaaaa
     5941 tcggaatagg ccgaaagggt aaaaatatca tcaatagttt ttacattcag gtaagcctct
     6001 aaatctttgt acaattgtgg taaattttca gcaacagcag ttgttttagc tttttcaaag
     6061 gaattaatat tgtcaataaa ttttggcagg ttgtcatgga ctaagcggta tgcaatggct
     6121 gttgattttt cctcggccga atacatattc ttcctgtttt cgtgaaaccc cgtaaaatag
     6181 gtggtaaaat tttcaaactc agacacaagt tgccaatctt tttcatcttt cacaaaggtt
     6241 ttaaggtctt cctttatcag ctctttagca aatagcctct tgaactggtc tttaaaagca
     6301 ctgtcttttt cgaaccgctc agcaatttgt tttcggagtt ttgcctgaac atcttcaaac
     6361 ttcttcttta agttatcatc tctttgtttc tgcatgtaga gagaataata ctcctctaaa
     6421 gaatcatttt tgttttcact ggcatacttc aatttaaacc ccgtcaaagc tttttcaata
     6481 aaagccttgt gataatcatc tataattttt ttcacttcct ggtaactttc agcccgatgt
     6541 tcatcttgga ttaaaaatcc attcttttca atgtgttcca aagtttttcc ttgtggcacc
     6601 aactcaaacc tcaaagtctt cgacaacgaa taaagtcctg taaattctga taaatctttt
     6661 ttcatcataa taaaatttaa tttttaaagg aatgatggaa ctcccatgtt gacaatttta
     6721 tttctgtcgt gtttgcgttc gcaagaacat gggtgtttca taggtttaat atttaaatta
     6781 tttgtaaatg tcatttctac gttcaacatt aaattatact acacaccata ataattctac
     6841 aagttacaaa atattacaac attcattttc ttcaaaaaac gctttgcatc ttgaagatgc
     6901 aaagcgttta agcgtttacc cgttcttttt caaatacacc actacgccta cctggttttc
     6961 ggggtggtcg gtggcattga tgcgctggat gcgggtttcg aatatttcgg catagccctg
     7021 ttcgaggagc ttgcttatgg ggctgttttc gccacgcggt acaaaaccca gcttatgctc
     7081 tccaaagtaa agcgccactg cctttgggtc gtatctgttg tcaggctcgg ccaccagacg
     7141 taattctgtg cctattttta actcattgaa tactatgggg ccatcgtaat aggtaaaccc
     7201 tgcaatgttg aaatgtgtca aatgtctgcg ttccattgtt ttatagtttt aatctggaac
     7261 aaaactattg tacccaagcg tcagtttctg tcgttatatt taatcaggta atttattttt
     7321 tcatggataa atgcttttag tttttcatcg ccaagtattt gtatatcagt gtataaacca
     7381 aggataaaac gccccacgcc ttcgtagctg cacaccgggg ccgaaaatat aaagctgttg
     7441 tggttttggc aattgacata ttgctcggcc agcggatact cctccaccag caggttgtag
     7501 gcacgtatgt tgagtttcag ctcggctttg ccaatatagt cgtgccctga aatacgaaaa
     7561 acatctatct gcggcaactg gtgcagcttt tcgttttgcc agttgttggc aagcacttct
     7621 acctcgccta ttcgcgacac tttaaacagc tttgagcttt tcgaagctgg ctcaaaacac
     7681 catacctgct gataattggt ggtaaagcta tacggctcca cctgtcggtc ctctacggtg
     7741 ttgctgttgg acgagcgata gtttttcagc accgcgcaac gcttatcctc aatggcctca
     7801 atgagggcat gtacattctc gccctgcccg ggcttgacta tcacatcggc cagccacggg
     7861 taatggtaga tgttgtaaag cttggccttg aggttttgct tgagggtact gccatcgtca
     7921 ataccatcaa tggctttgtg caagatgtag gcctcttcct ctgtaaaatg cacaaggtcg
     7981 ctgagtttct tcatacgccc gtcgttggga ttgaggtatg gtatgccgcg cgaatgatat
     8041 tcaacaataa agcctgcggc acgaaaggta tcaatataac gctgaacatt cctgactttt
     8101 gtgtccaata tttcagcaag ttcattagta tttcgcctgt tgccaataag caaaggcaat
     8161 aaacgcaaag cacgttctat cctgggttgg tccataataa atgagttttg ataattataa
     8221 cgattaaaag tataaacaaa atcttatact tgttagaaaa ttctgaaaaa actaacacag
     8281 ttgaaattta aatccaaaag atccgcaaaa aaaactacct ttaccaataa tccggcacga
     8341 agatactatt gcacgtaatc cactatcagc aatagattac aaagtttttc atctgttaac
     8401 tccagcgaat agaagcattg catcgttttt tataattaat aaataaaaat tcaacgtcgt
     8461 ttagtaagta ttaaaaaaaa acgataacag gtctgaaaga cgctgtcggg ttagtttcac
     8521 ttctgacctg acagcattcg tagaacttgt tagagtctta actaaacgac attgaataaa
     8581 aatgagtatt aaatgtttga aaagaaaaca acgtttaaat cacccgatct cggggaattg
     8641 caggaagtaa aaatcgattt caaaacaaaa atttatattc ctttgggtaa aaatcctgaa
     8701 gaagccaaaa ggcggatttt agaaagagaa gctgaaaagt cagattcaat agcttttcgc
     8761 aaaaagcctg taactgcata aagtttaaca ttattgaaat gtaaagcgta gttttttgaa
     8821 gcaaacaact gcgctttttg cttttatcag gctacatatc tctaaaaatc caaatattct
     8881 ttataatttt tagctgtaac acctgtaatt acttcttgcg tatcaccctg ataaacaata
     8941 tttttttcaa taaaaggcaa agatagtttt tcaatagttt tatgaaaaac aggcttatat
     9001 gttacagatg cttttatttc aacacaacta accttctgtc catcttcaac aatcaggtca
     9061 atttcatctc catgacttgt acgcaaatac caaagatttg cagtatcgcc attatggtat
     9121 atatttttca gtgtttcaga aataatcaga ttttcaaaaa gagccccata aagtacgccg
     9181 tcctgccatt gctccatagt tttaattccg gttaaaaaag ccagcaaccc attatcataa
     9241 aaatagactt tgggagtttt taccaaccgc ttaccaatat tggcatagaa aggtttcaga
     9301 agaaaaatta taaatgaagc ttctaatacc gatacccact ttgaaagggt tgacactgta
     9361 ataccagttt ctttagcaat atcagacaag ttaagttgtt gtccaacacg tgctgccaac
     9421 agttttagca tcagagtaaa tgcattaata tcaacaatat tgagcatttg cctaagatct
     9481 ttttgaacat aggtattgac atatgcatta aaaaagttgt gagtctgctt aaatgaccca
     9541 gacaacaact cgggaaaacc gccattaata acaaatgttt cctgtaacga ttttggtcct
     9601 tccttatact gcattggcaa gagagagatt agcccaatac gcccagccag actctcacta
     9661 atatttttgg ataataaaaa ttgccctgag cctgtcaata caaattgtcc cctttcctca
     9721 ggattattat caacaatttc tttgattcgt tggaagatat ctggcatttg ttgcacttca
     9781 tcaaaaataa catgctggtt gtattttctc ataaaagcaa caggatccgt ataatataaa
     9841 tttcgaagct caatattatc aaaattcaca tatacataac gatctccaag caattgcttt
     9901 accaaggttg attttccaga ttgtcggggg cccattaccc caaccaccct gaattgcgaa
     9961 atatattcgc ccaaaagtct ttctgcaagc ctgttataat acattttgta caattttata
    10021 atgcaattat aaatttacac aaatatataa taaaatattt aaaatcattg ttatctataa
    10081 aaatcatgaa tcaaaaaatc cattaatcta ttcatcaaaa acatgccatt gcttcaagaa
    10141 tattgaatac ttttgcacgc tttttaaaaa ttatatataa taaatgatta cagttaccaa
    10201 ccttggcatt cagttcggaa aaagggtttt gttccaagat gtgaacatga aattcactcc
    10261 cggcaattgc tacggtatta ttggcgctaa tggtgccgga aagtccacct tcctgaaaat
    10321 gttgtatggc gatgtggaga atatgcgtgg ttcagtgaca ttaggccctg gcgaacgctt
    10381 gtcggtacta aagcagaacc actttgaatt tgacgaatgc actgttatca acaccgtatt
    10441 gatggggcac tctgaacttt ggggcattat gcaggaaaag gagacccttt atgccaaagc
    10501 tgatttttcg gaagctgatg gcataagggc ttcagaactt gaagaacgtt ttaacgaaat
    10561 agatggctgg aatgccgaaa acgaagccgc cagtttgctt agtggccttg gtgttaaaga
    10621 atacctgcat agcaaactga tgaaagacct tgaaggcaaa gacaaggtga aagtattgct
    10681 ggctcaggca ctgtttggca aacccgacaa cctgctgctc gacgagccta ccaacgacct
    10741 cgacatagac accgtcacct ggctggaaaa ctacctttca aactttgaaa atactgtgtt
    10801 ggtggtatcg cacgacagac actttctcga tgctatttgc actcatactg tagatattga
    10861 ttacaacaag gttcagctct ttgctggcaa ctacagcttc tggtacgaat cgagccagct
    10921 ggcattgcgc caacagcttg cccaaaacaa gaaagccgaa gagaaaaaga aagagctaca
    10981 agagtttatc caacgcttta gtgccaatgt ggccaagtca aagcagacta ccagccgcaa
    11041 aaaaatgatt gaaaagctaa acattgaaga aatcaaacct tcaacccgca aatacccggg
    11101 tatcatcttt acccctggcc gtgaacccgg caacagtatc cttgaagtaa aaggcttaac
    11161 caaaagtatt gatggtactg tacttttcaa agaccttact ttcacagtca ataaaaacga
    11221 caaaataacc tttctttcaa aagatacccg cgccattact gcactgtttg aaatactgaa
    11281 aggccacgac aaacctgata gcggcacttt ccaatggggg caaaccatcg tgaaggcata
    11341 cctgccactc gataatgcca aattctttga aaaagacatc aacctcatcg actggctagc
    11401 ccagttttcg gaagacacca gcgagctata cctcaggggg taccttggca agatgctttt
    11461 ctcgggcgaa gaaatttaca aaaaagctaa cgtcttatcg ggaggggaaa aagtacgctg
    11521 catgattgcc cgcatgatga tacgcaatgc caatgtaatg ctgctcgact cgcctaccaa
    11581 ccacctcgac cttgagtcga tacaggcttt taacaatgct cttacaaaat tcaaaggcaa
    11641 catcctgatg acctcgcacg accacgagtt tatccagaca gtttgtaacc gctttatcga
    11701 gttttcgccc aaaggcatga ttgacaaaga aatggacttt gacgactacc tctcagacga
    11761 acggataaag gaattgagag agaaacttca ctaaaatgaa atagaattag gcttttcata
    11821 atattataaa cgcagagaac cgcaaagttg aatgatcttc tttgcggttc tctgcgttaa
    11881 aatactttat cagatattag aaaaaggctt acgtatttgt catttacaga cattctttac
    11941 ttttgccttg aagcaaaagt aacaaaaatt caaggcttgc cgtaaattgg ctaaaaaaag
    12001 ctttatcgac ggctccgggc aacaactcgc ttcgctcaaa cagttgccct cacttcgccg
    12061 ataaagcttt tttcttaacg ccatttacgt caggccataa acagaaaagc tcaatgaaac
    12121 aggcgaagcc ttaaaagaaa aattcctttt catcgccttt ttgttcgatt tcggccgaga
    12181 agcctaacat tcctgtggtt tgcatattgc aacattactt ggcttcgagg gtgaaagagt
    12241 taaggcgaaa agagtttttg aacaactttt ttcgataaaa aaattgcata gagacataat
    12301 ttattgctat tcaatacttt ttaaacaata attgacaatg ttattctttc gtcgaattga
    12361 cgttaattaa accccaaact ccttaataac cttgaacact ccgttgatga acatctgaac
    12421 tgcaataaca gtaaggatta aacccattat tcttgataca accttaataa gagaagggct
    12481 tagcttatct gcaatctttt tacttataat aaacataaag aaagtaatgg tacaaacaac
    12541 agcaaaaaca agtattacaa gcaatgttat taaaaagcta tttccttcgc caacaaagtt
    12601 catggcagaa gatatcgtcc caggcccggc cagcaatggt atccccaaag gtgaaatagc
    12661 catatcgttt aattttgctg tatcattctc catttttgtt tggtgtgcac cggcagcttt
    12721 accttgtaag aggtcataac caataaaaaa aacaataatg cctccagcta tctgaaaggc
    12781 aggcaaggta atgccaaaca ttctgaatat cacgtgccca aacaaacaaa acgtggtaat
    12841 gataataaaa gctacaaaaa cagccctgaa tgcaaacttt ttaatcactt tgtcttctga
    12901 ttcgccaacc atggaaataa aaaccggagt atttccaata gggttcagta tagcaaagaa
    12961 acctaaaaat actgttgtca gaaataggct taaatcgtgc atagcaataa ggttttagaa
    13021 atgcaaagtt aattaagaga agctactcct tccaacaaaa atcatttact attaagctct
    13081 actttaacat gcccccaatt ttcgccccgc ttaatacgtg taacctgcat ttcgctaata
    13141 caaaacattt tagctatgtc aacattctgt acaccccttt ggagcatctt cttaatagca
    13201 ataacatccc gcaccttcaa tttgctgtac gttatttgtc taaccctcgc ctcaggcgga
    13261 atttttgctt tcaactgtct gtggtgctca tccaactgtt cttttgtcat ccagaccagg
    13321 ttcgagatat ggttattctt caggttgccg tccaaatgag ttacaaacgt atgttcttca
    13381 gatggttttg tcagccaaac ctctgccaac aatttgtgca aataaagtct ttgttttttt
    13441 tcccctttaa actgaatttc aacaagttta aaaccattcg tttctccacc tttaagaatt
    13501 gccccttctt ttttataaac aaatgatttt agcctgccat aattacttaa aaaatagcgg
    13561 ccattagacc cgggtacttc tttccaaatc tcatcctcaa tttttctgag ctttctttga
    13621 gtattcaaca tttttttaaa ttatgtaaaa gtactaaaga tgaaatacaa atgataaatt
    13681 accaggattt taaataagcc atactctttt cttcataaaa aaagccagct ttttccactg
    13741 gcttctattg gcggagaaag agggattcga acccccggtc cgagtaaacg aacaacggtt
    13801 ttcaagaccg ccgcattaga ccactctgcc atttctccgg gggcaaaagt agtatttttt
    13861 gtttatttgc caaagaatcc ggtaaaattt tctaaaaatt ttaattcact aaatatcaat
    13921 actttgaact ctaaaaaata ttacttcatt ttatttaagt caaaaaataa ccaaacatca
    13981 acaaaaaacc tgtcaggttt ttacaccaag acaggttttc caaaatgttt ttagactttt
    14041 tgttttttta acttccagtc aaaactcatt attaacttaa aatcactcta cacttatctt
    14101 attagtaaat aaaatgtttt cagcagatgt acccacctca atcagatatt ctcctggctc
    14161 tagcgtccaa ctaccttttt tctcatcata aaatgaaaga ttttgggcag ctacactaag
    14221 ttcaaccgta ctggtctggc caggttcgag ggctactttc ttaaaagccc tgagttcgcg
    14281 caacggacga tcaaccacag actttggctt tctgacatat aactgaagca cttccgatcc
    14341 tttaacttta cctgtgttgg taatattggc ctttatggta aaagtttcat tggcttttac
    14401 tttctgtata ccagaagtgg caggttttat atcaaaagtt gtatatgaca ggccataccc
    14461 gaaaggaaac aaaggcttaa tcttttgggt caggtaccat cggtaaccta cgtatatccc
    14521 ctctttgtat tcctggttga tactatcgcc cggatatgat atagccccat tgcgtatagc
    14581 aggacagtct tccagcttaa ccggaaatgt aaatggcagc ttacctgaag ggtttacatc
    14641 acccgaaata acatcagcta ttacatttcc agcctcactg cctatgtacc atgcctgaac
    14701 aatagccggg accttattta tccagggcat gcttactgca ttacctgtaa gcaataccat
    14761 taacatattc ggattaactt tcagaatttc gtttatcagt gaatcctggt tatatggaag
    14821 atccatcgac ttgcggtcgc caccttcgct gtcctggaaa aagtttttat tcagccctcc
    14881 aacaaaaata accaaatcag ctttcgaggc agcttcaata gcagcttttg caagcgaatc
    14941 agcattaaat ttaagctcag gctttttccc ccaggcaaat gtcgggcttt cgtatcctct
    15001 tgagtaggta atagcagcat ttttaaacct tttctgtatg ccatctaaag gagaaacttc
    15061 atataaagcc ttaagctcag atgaacctcc gccaggtgtc ataggtttga cagcattttc
    15121 gccaataaca gctattttta ttgtttttga agcatctaaa ggaagcattt gattattgtt
    15181 tttgagcaat acaattcctt ctcttgctat gttacgtgct attattgaat gttctcctcc
    15241 aattttgcca ataggcctgt cggttcgcat gtttgttttc aggcaaagcc tcaaaatcct
    15301 tctgacctta tcgttcagca catcttcaga aacttctccc tttagtaaag cttgttcaaa
    15361 gggttttgca agataatatt cgctaaaatc aaagatggta tcccaacgtt tgccccaagt
    15421 ccccatttca atatcgaggc cattgtttgc agcttcaagc gtattgtggg tagcgttcca
    15481 gtctgacata acaactcctt tgtaacccca gtcttttttg agtactttgt taagcaaaaa
    15541 gtcgttatgg ctacaatgct gcgaattgta tttattataa gctcccatta gggtccatgc
    15601 atcgccttcc tgacatgcag ccttgaacgc cggaagataa atctcgtaca aagccctgtc
    15661 actaaccttt acattgatat ggccacgcca tctttcttgg ttattaaggg caaaatgctt
    15721 aatacaagct gcaaccccaa tagattgaac tcccttaata taaggaactg ccatttgggc
    15781 tgccaacaat gggtcttcac ccatatattc aaaattgcgg ccatttaacg gatgacggta
    15841 aatgttgact cccggggcaa gaataatatc tttatttctg aaacgcgctt cttcgcctaa
    15901 ggcaacacca tattcataag ccatttgcgg gttaaacgta gcagccagac aggtaagcgc
    15961 cggaaaagca ataacagagt cgtttgtcca cttagcatag tcccacgtag cccaacttat
    16021 ctcagcccta accccatgag ggccgtcaga catcttcaaa tctggaatcc ccaaacgagg
    16081 aacaccacga gaactgaatt tactgttagc atgacacatc ctgactttct cgtcaagagt
    16141 catctgcttt atcagtacat ctattttctt ttcgatgatg ctgtcagctt cttgtgcatt
    16201 gacaaaataa aatgacaaca aaaaagcaag ttgtagtaat attaaccgtt tcattagttg
    16261 tatttattta gattcaaaaa tcaaaagtca agaaccaaaa aatattcttc accctatttc
    16321 aaatgcaaat aaatatcaaa tcaatgaccc aaacagttaa tgaatagtta acggcattag
    16381 taaatgataa aattcagtca aattaccaat aaaaacagag tattcaaaca tatcaaaata
    16441 acccttagcg tattttagac gcaaccaacg ttgccctaaa ttgctaagct ctaatcattt
    16501 ctatataata tcgccgtatg cagctaaata aataactcgc agacttcctt taccttcttt
    16561 ctcttcctca atcttccttt atcttcctca atcttcttca atcttcctca atcttctctt
    16621 ctccgcttcc tccataatag ccgcagtagc cgaagccccg cacctgtcgc aacctgcctc
    16681 acgaactgca atcagttgtt ccagtgtccg cactccaccg gcagctttta cttttacttc
    16741 cgcaccgcaa tgatggcgca tcagctttat attgctaata gtagccccct gatagtcgta
    16801 cttcccgtca gcacccttta caaaaccata accggtagat gttttcacaa aatcagcttt
    16861 taccttggta catatttgac aaagcttaat aatatcagct tctttcgtta caaaatctgt
    16921 ttcaaatatc acttttacaa tagctccatg cttatgacat gtttttgtaa cagcatcaat
    16981 ttctttttct acataatccc aatcgccttg taaagccttt ccaatgttta tcaccatatc
    17041 aacctctaca gcaccatttt tacaagcttc ttcagtttca aacactttag atgcaatggt
    17101 tgaattgcca gccggaaaac caattacaca ccctaccaat acatctgagc ctttcaacaa
    17161 ttcttttgcc attggcacca tatagggttt cacacataca gatgcaacat tgtattttac
    17221 agcaacggca cattcttttt tcaaatctgc atcggtaagc attggatgca ggatagaatg
    17281 atcaatcatc ttagctaatt gagttattga agtcttcatt tttatatttt tattgatatg
    17341 caaaaaaagt acatttgtgc catataaacg ttgcaatgtt gtttttatga taaaaatcct
    17401 gttgataatt ggcacaggca gttttattgg aggtatcatg cgctttttag catcacgtta
    17461 catccaaaac gctattattt cagcgtttcc atggggaact tttttagtca acgttgttgg
    17521 ttgttttctc attggtcttt tttacggact atctgaaaaa aacaatatag ccagtactga
    17581 gctgcgaatg ttccttactg taggtttttg tggtggattt accacgttct ctacttttgc
    17641 caatgaaaac cttgccttat tgcgcaatgg cgagtttttc tatttcgcgt tgtacgccgg
    17701 gttgagcgtt tttattggct tactgtttac atatatgggc aatatgacta ctaaactctt
    17761 ttaactatga acacagctgg cgaagcaaag gttttgcgta tatacatcag caacaccgat
    17821 aaattcaaac atgctccttt atacgaaatg atagtgtttg cagcaaagcg atatgggctg
    17881 gcaggggcaa cagtaaccaa gggtattatg ggttttggct cttcgagcgc gatacattac
    17941 attaagtttt gggagtttac cgaaaaaatc cccgtagtgg tcgaaattat tgatgaagct
    18001 aaaaaaatag atagttttat taaaacaata acgccgtggt tcgaactaat aagttatgga
    18061 tgccttatca ctgttgaaaa agcaaacatc gttctttata aaacaggtaa aagaaaaaag
    18121 atttcaatta tataaattat ggagaaaaat aagactattg gccttgtggc acacgacaac
    18181 atgaagcaca acctgattga atgggtcgaa tacaactggg aaacattgaa aaaccatcga
    18241 ctggtatgca ccgggactac agggcgtttg gttgaagaag ctatcacgaa aaaaatggag
    18301 cattccaagt caggcgttga aaatattcac atacttaaac ttaaatcggg gcccctggga
    18361 ggtgaccagc aattgggcgc tatgatttca gaaggaaata ttgatatcct tattttcttt
    18421 tgggacccaa tgcttccaca gcctcacgaa gtggatgtca aggctttgct tcgcatttgc
    18481 gtagtttaca atgttccttc tgccagcaac agggctacag cagattttct tatttcatca
    18541 cccttgttca acggctatta tagccccaaa aggattgatt acagcagcta taccaacagg
    18601 aaaatttagc ataaggcctt tctgggaagc aacttatcag gtattatatg ttgtttctca
    18661 agataaagat atcaaacctc cctattgaaa aggtttaata aaagctaaaa agaattttta
    18721 aaaattactt gctttttaaa aatatatttt cgtcttttgt aaaaaagaaa taagaaagtg
    18781 aaaatacaaa tgaacatatt ttggtggtgg cgcaattctt tcatttaccg tgagaggaat
    18841 taggccattg taccatttaa tgatatagca aaaggcttac caatcacggt aagccttttt
    18901 tgttttaaaa ttttaaaacc ttgaaaatta aaagatatga aattcaaaat tgaagtagta
    18961 acccgtcaaa tacttgccga caccattacc ccggtgagtg tttacctcaa actgcgcgat
    19021 gtgtttccat acaccatctt gctcgaaagc tctgactatc acggaagcaa aggaagctgg
    19081 tcattcattt gctgcaagcc tatagccacc tttaaagttg aaaaaggcaa gatttatgag
    19141 cgcttcccta accaaacatc ttcagtttct cacattaatg acgaatttag cgttgcagat
    19201 cgtctaaacg cattccgcaa tgcctttgat tgccaatcaa aacaagatgt acccattaat
    19261 ggtatcttcg gatatgccgc ttatgatgcc attgagtatt ttgaaaacat aaaactcaac
    19321 accagtatta gcgaagaccg cgaaatccct gagatatttt atagctttta tcagtatatc
    19381 attgccatca accagtttca caacaaactg tatattatcg agaacctgat gcagggcgaa
    19441 aaagacagta cagaccgcat tgagggactt ttgcaaaacc gcagctatgc catgtaccct
    19501 tttaaatctg ccaacaacga aacgtcaaat aaaacggatg aagaatatat gaacatggta
    19561 acgcttggaa aacaaagctg tcagcgtggc gatgtatttc agatagtgct ttccagaagg
    19621 tttacacaaa cttttacagg tgacgaattc aatgtttaca gggcattacg ctcaatcaac
    19681 ccttcacctt acctgtttta cttcgactat ggcagtttca aaatatttgg ttcatctcct
    19741 gaggccgaaa ttgagattaa aggaggtaaa gctgctattc accctatcgc cggcacattc
    19801 aagcgtactg gcaacgatga acaagataaa atcctggctg agaagctttc gcaggacaaa
    19861 aaagaaaatg ccgagcatgt tatgttggtt gaccttgccc gtaacgacct aagccgcaac
    19921 gccagtaatg ttacggtaga aacctatcgc gaaatacagt actattcgca tgtattgcac
    19981 atggtttcgg tggtatcagg ccaattacct gacaattaca accccgtgca aatcctggcc
    20041 gattcatttc ctgcaggaac attatccgga gctcctaaat acaaggctat gacccttatt
    20101 gacaaatacg aaggtgacag acgtggatat tacggaggct gcattggttt tttgggcttc
    20161 aatggcgact gtaaccatgc catcatgatc cgctctttcc taagcaagga caacaggcta
    20221 ttttaccagg caggtgcagg tattgttctt gagtctaaag aagaaagcga gttggctgaa
    20281 gtaaataaca agctggcagc cttaaagtcg gccatagtat tagcagaaaa ccttaaataa
    20341 ctgataacga tgaaaattat agtgttagat aactacgatt cctttacata caatcttgtg
    20401 cattctattg aaaagttcgc taacagcaag attgatgtag tgcgaaatga caaagtcgat
    20461 gtagaatact ttgcaaaata tgataaaata gtgctttcgc caggccctgg tatcccagac
    20521 gaagcaggca tacttaaacc tgtaataaaa agatgggctg cggaaaagag catccttggt
    20581 gtttgccttg gattgcaggc tataggcgaa gtatttggcg gaacacttga aaaccttaat
    20641 aaggtatatc atggcgttgc atcaactatt atacaaacag tacctgacga aactatattc
    20701 catggaatac ctgaaatatt tgaggctgga cgttaccatt catggattgt caacaggaat
    20761 acattgcctg aagaattgga aatcacagcc atagataaac agggacaaat catggctctt
    20821 cgccataaaa aacacgatgt acgaggagtc cagtttcacc ccgaaagcgt ccttacccca
    20881 atgggcgaca aaatgattga aaactggttg aatagttaat tacgaataac caattacgaa
    20941 ttacaaatta ttttttaata gcgtttaata ttcattcctt ggacaactct tttataaagc
    21001 aggtatttcc tatcttttat aaggagagct cgtcccgcct caggcgggag agaggaatag
    21061 taaaaagaaa tattatgaaa aatacattaa actacttgta ctcgcataaa acattgaccc
    21121 gtgaagaggc caagactgta ctgaccaata ttgctcaagg tcaatacaac cattcgcaga
    21181 tagcctcatt tttaagtgtt tacatgatgc gaaacattac tgttgaagaa ttagtaggct
    21241 tccgcgatgc attacttgag ctatgccttg ctgtaccaat ggatgaatac aacactatcg
    21301 atttatgcgg aactggcggc gatggtaaaa acacgtttaa catttctaca ttatcttcgt
    21361 ttgtggttgc cggcgcagga tacaaagtaa caaaacacgg aaacattggt gtttcatcag
    21421 gttgtggctc atcaaatatc atggaaagcc ttggctataa attttcttct gaccatgaca
    21481 aacttcgccg cgaaattgaa aaaaccggca tttgctactt acacgcacca ttgttcaatc
    21541 cggccatgaa aaacgttgct cccgtgagaa aagaccttgc cgtccgtacg tttttcaata
    21601 tgctcgggcc tatgattaac ccttcattcc ctaaaaacca gattgttggc gttttcagtc
    21661 tcgaacttgc cagaatctac acctatattt atcaacaaag tggcataaac ttttatattt
    21721 tgcacagttt agatggctat gacgaaattt cgctcacctc agactttaaa gttattggaa
    21781 acgaaaaaga ccagatactt tctcccgaaa gcctaaattt gccgcgtatt aatcctgaag
    21841 acattaccag cgataaatct gtggaagaat ctgtaaagct tttcttaaat attatcgaag
    21901 gaaaaggcac tccggcgcag aataatgttg ttgctgtaaa tagcggcctt gctattcaat
    21961 gtttgaacaa aaataaaaaa ttggaagact gtattgaatt ggcccgcgaa gtattgatct
    22021 ctggaaaagc caaacaagta ctgaaacatc tgattgaaat gcagtagaag gcaaaaggag
    22081 ataaggaaaa agtaaaagaa gaattaataa taacaggcac ttacttgtaa aggagcagca
    22141 tttcccctcc tttataagga ggggccaagg gtggtaaaat atatgacaat tcttgataaa
    22201 ataatagaga ctaaaaagca agaagtagct gaaaacaaag caaaaacgcc tgtttcaaaa
    22261 ctcgaaagaa gccaatactt cgaacgtaaa tgtatttcct ttcggaaaaa cattgaaatg
    22321 caaggcacat caggcattat tgccgaatat aaacgccaat cgccatctaa agggataatt
    22381 aacaacaatg taccagtaaa agaagttgct gaaggttatt tcaaagcagg agcttctgct
    22441 atgtctattt tgacagacaa ccaatacttt ggaggctgca acaacgacct tttagaagga
    22501 cgttcagctg ttggtctgcc tattatccgc aaggaattta tcattgacga ataccaaatc
    22561 gttgaagcaa agtctattgg tgcagatgcc atattgttga tagccgcatg ccttactgca
    22621 ttagaaataa aaacactaac ggattttgcc cattcgctgg gtcttgagat attattagaa
    22681 ttacatgacg aaagcgaatt gaaaaaggta tatgaccatg cagacatgat aggcattaac
    22741 aaccgtaacc tgaagaactt cgttgttgac gtggagcatg ctataaaaat ggcccattca
    22801 ttgccaaaaa aaatgaccaa aattgcagaa agtggacttg acagccctca cacagttgct
    22861 atgctccgaa aagaaggatt tcatggcttt ttgatgggtg agcattttat gaaaacatca
    22921 aaccccggac tggcatgtca aaacttcata accgaactac gaaacattga aaaacgctaa
    22981 tcatgcaaaa aaaattaaag ataaaagtat gtggcctgcg tgatgaaaaa gatatactca
    23041 agctatccct tatggatatt gattttatgg gtttcatctt ttacaaacct tctccacgct
    23101 atgctggcga aatgcttaaa cctgaaagaa tagaaaatat acctgaaaga attatcaaaa
    23161 ctggcgtttt tgttaatgaa aaggcagaag acaccatccg tatcgcacaa aaataccatt
    23221 tacaggccat tcaactacac ggcaacgaat ctaccgactt gtgtaacgag cttaaagaaa
    23281 agggatatat tgttattaaa gcattaaaag gaaataaaga agatttagta acaagttcaa
    23341 tattgtataa agaggtttgc gattattatc ttatagatac ccccacagtt cagcacggag
    23401 gaagtggcca aaaattcaac tggtccattt tgaatgagtt cgagtttaac aaacctttct
    23461 ttcttagcgg tggtattggc ccacatgata cagacatcat aaattcactt aattttagag
    23521 acttatacgc tgttgatata aacagcaaat ttgaaacatc acctggaaaa aaggatacaa
    23581 ccatcatttc tgaattcatt aaaaagataa aataaaaaac tatgacagat aaaggaaata
    23641 aagattacat ggctgatgaa aaaggcttct atggacaatt tggaggagcc tatatccccg
    23701 aaatgcttta tcataacgtt gagcagctca gacaacaata caaacccatt attgagtcag
    23761 cttcttttca gcaagagttc aagaaactcc tcaaagacta tgttggacgg ccttctccat
    23821 tatattatgc ccaacgcctt tctgaaatat ataagaccaa cgtctatctg aaacgtgaag
    23881 accttaacca cactgggtct cacaaggtaa acaacaccat tggccagata ttgctagcaa
    23941 aagcaatggg caaaaaacgc atcattgccg aaaccggagc cggccaacat ggtgtagcta
    24001 cagccaccgt gtgtgctttg atgggacttg aatgtgtcgt ccacatgggc gaaattgatg
    24061 tcaaacgcca aatgccaaat gtacttcgca tgaaaatgct tggtgcacaa gttatccctt
    24121 ctatgagcgg cagccgtaca cttaaagacg caggcaacga agctttacgc gactggatta
    24181 acaatcccga agatactttt tatcttatag gctcggttgt tggccctcac ccctaccccg
    24241 atatggttgc ccgcttccag tctgttatta gcgaagaaat aaaatggcaa ctcaaagaaa
    24301 aaacaaacag tgagcttcca aattatgtaa ttgcctgtat tggcggagga agtaacgcta
    24361 tgggcgcttt ctacaacttc ttaaatgaca cttctgtaaa actaatagcc gtagaagctg
    24421 caggaaaagg agttaatact gatgaaacag cagcatctat cgccaaagga agcgttggca
    24481 ttatccaagg tgcaaaaact tatctgatgc aaaccaacga cgggcaaatc attgaagcat
    24541 actccatttc agcaggactt gactaccctg gcattggccc acaacatgcc cacctctata
    24601 acattggtcg tacacaaata atggatgcca cagaccatga agcacttgaa gctgcgtttg
    24661 aattgacccg cgtagaaggc attattcctg ctcttgagtc ttctcatgct ttagccgcat
    24721 tgaaaaaact aaaatttgaa aaaagtgatg tggtagttgt caatatatcc ggacgtggcg
    24781 acaaagacat ggatacttat attgcaaata tggacaaata ttgccagaaa taaatcatca
    24841 aaatatgaac aacatagcaa taaccttcga aaataagaaa aataacgtgc tgtcgattta
    24901 tttcacagct ggctatccaa aattgaccga cactattaaa atacttgaaa tcctgcaaga
    24961 tgcaggagct gatatggtcg aaatcgggat gcctttctct gacccccttg ccgatggccc
    25021 tgtcattcaa aatagcagca ctgttgcact taaaaatggc atgagcattc aggagctttt
    25081 cgaccaactc cacggcatgc gcaccaaaat tacaatgcca gtaatcctca tgggctatta
    25141 taacccagtt ttcaaatatg gcgtagaaaa tttttgtaaa agtgcagcca aagttggcat
    25201 tgacggccta ataattccag atctcccaat tgatgagtat ttggcaaaca aacccatctt
    25261 tgacaaatac gggctttgta atatattgct tattaccccg caaacaactg atgaacgtat
    25321 taaacaaata gataacatcg ccagtggctt tatatatgca gtatccagct atgctaccac
    25381 cggaagcgga aaagcattaa acctctctgc ccaatacttt gaaaagttga aaaacactgc
    25441 ttttaataac ccagtcatga taggctttgg cattaaggat aaagcaacat tcgataatgc
    25501 ctgccaatac gccaatgggg ctattatcgg taccgaattt attaaacttc tggagaaata
    25561 tggcgcagac aaaacaaaga ttactgagtt tattaagggg gtgaaaggga atgaccaatg
    25621 accaatgaca aattacaaat tacaaattac aaatgaagaa tgaagaaaat catcatctta
    25681 acaaactcgg ttcctattag gcagtatttc ccctcctttt catgaagagc ttgtcccgcc
    25741 tcaggcggaa ggttaaggtg gttattaata aaaaagccgg atgtatcaac gacttccggc
    25801 ttttgatttc ttcaacaaaa caattattct acattctttt caaaatcctt tttgtctttg
    25861 tctatttgct cttgtttttc ttttgaaact gtctttttag ggcttgagca tgcaaatcca
    25921 aacatgcata caacagccag aatcagggct acttttttca ttttgtttaa atttgtttaa
    25981 ctgaaaacaa agataaacag taaaacaaaa gtaagcaaat caattttggc acaatccaat
    26041 ctctttcata aaactaatgg ctttttcatt gtttaaactg gctgcttttt tcagatcttc
    26101 gctcagccct ttttcatcgt taatagccat tttaaccaat cccctgttga aatatgcttt
    26161 tgaataactc gaatctgcat gaattacttc atcaaaatca ttcaaagctt tattatagtt
    26221 atttaataaa aaaaatacta ttcccctgtg attgtaactg tccaaattat caggcaaaag
    26281 cattaatgct gtattaaaat cagccaatgc ttttcggtac ttttcaagct tagtataaca
    26341 aaccccgcgt ttatcataca agactgcatt ctcactatca aagcttaatg caacagtgta
    26401 ttcttcagca gctaagtcat aatttccggc ctgctcatat ataagcgcat gacctgcatg
    26461 taaaacagcg ctatccttat tactctcctg ggcaaataca cttgaaaaca acaacataaa
    26521 cagcccaatc attccacctt tcaatatatc ttttgttttc atagttagat atttttattt
    26581 tatcttacag caaagatatt attattattc acttcaaaac aattacatta tattgataaa
    26641 aagcttatgc aacggtgatt tacatcatca tcaccccaaa aaaatatatt ttatttaatt
    26701 attaatcaat taattaatca aataaaaagg gggattttaa accaaaatca agaatgttta
    26761 ctaattttat ttgagttttc atgcgcaaaa agctactttc gtagcttaaa tataaaatat
    26821 gcatatataa caaatgagct ctgacaatca acactctgtt caaaatgaac aacaaggtat
    26881 tgccaataac aatactgaaa atacgatgaa tacttattta cctgaaaatg gacttactat
    26941 ggaagacaaa atgaaagaca tcgttcctca ggaacaagaa acccaaaatc aaagcaacgg
    27001 actcgaaatt gatgagtttg aaatggaaca catgagcaac atccctgatg aaatgcttat
    27061 tccggaagat atgcatgatg acgatgatga acatgaagag cattcagctc ctgttacctc
    27121 tgaaattgat tatagcaagc tgtcaaaaga agaacttgtt gccaccgccg cccgtttgtt
    27181 caacgaacgt gcgatagatc aactaaaacc tgattttgat gccataaaaa tacattttta
    27241 taaaattgtt aaaactgaac aagaaaaaat caaacgccag tttctcgaac aaggaggaac
    27301 attggaagat ttcaaagtag aacccgacat tttggaaaca aggttaaaag caatatttac
    27361 acaatacaaa gaaaagaaaa gcgcatatat ccagcacctt gaatctgaaa aactccataa
    27421 ccttaaccta aaaaaagctg tcattgaaga aattaaagat ttatgcaata gcgccgaatc
    27481 ggtaaacgac acctttaatc aattcaggga actacagaaa aaatggcgca gcattggccc
    27541 tgtacctcag tcagaagtaa acaacctttg ggaaaactac aaccaccacg ttgagaaatt
    27601 ttatgattac attaaaataa ataaagagtt acgtgatttg gacttcaagc gtaaccacga
    27661 ggctaagctc gaattgtgtg aaaaagccga agagctgctt ctcgagcctt ctattgttaa
    27721 agcctttaac cttttacaag aataccatgc caaatggcgc gagattgggc cagtgccttt
    27781 tgaaatgcgt gccgaaatat ggaaccgctt taaagatata acttccaaaa tcaataaaaa
    27841 acaccaggaa tattttgaca accaaaaagc tttgcaagaa aaaaacctgg aagaaaaaac
    27901 caaactttgc gaagaagctg aagccctttt aaatattgat aataaaagcg tcaaagactg
    27961 ggaagaccgt tccaaagcac ttttagaaat acaagaaaaa tggaaatcca ttggttttgc
    28021 ccccaaaaaa gacaacaaca agatttacaa acgctttaga aatgcctgtg atgcattttt
    28081 tgctaaaaaa cgtgattttt ataaagcact gaaagagcaa caaaacaaca acctgcagct
    28141 caaacttgat ttgtgcgttc aggcagaagc cctcaaagac agtaccgaat ggaagaaaac
    28201 aacagacgat atgttgcaac ttcaaaaaaa gtggaaagaa attggccctg tgccacgcaa
    28261 aatgtctgat gtagtgtgga aaaggttccg tgcggcatgt gatgtatttt tcaataacaa
    28321 agctgctcat ttttcaacca tagacaacaa atatgctgca aacctggcgt tgaaagaaac
    28381 tttgatttca gaaattgaaa attacagccc tgtagaatct gctgaagaaa acttcgaaca
    28441 tttaaaagca ttccaacgca gatgggccga aataggcttt gttcctatgc aaaataaaga
    28501 agaaattcag gctcgctaca aaaaagctat tgacaaactc ttcgagaccc taaaagttga
    28561 aaaaagcaag gttaacatgc ttaattataa aaacaaaatt gaaaaccttt ctcatagcaa
    28621 acattccgaa aagaaaattg acaaagaacg cgaaaaaatt atcatgcaaa taaaaaaact
    28681 cgaaaacgac atagttcaat atgaaaacaa tattggtttc ttttctaaat caaaaggagc
    28741 gcaactttta gtagccgaag tacaaaaaaa gattgatgac gctaaagctg aaatgaagtc
    28801 tttagaagaa aaaataaaaa tgatagacag tttataattt tatagccatg caatcaaagc
    28861 aaacatcttc tcaaccccac accacaaagt atattttcgt taccggaggc gtaacttcat
    28921 ctctcggaaa aggcattatt tctgcatctc tggctaaact tctgcaagca aggggctaca
    28981 atgttactat tcaaaagctc gatccataca tcaacgttga cccggggaca ctcaacccat
    29041 acgaacatgg cgaatgcttt gttaccattg acggagctga aacagacctt gatcttggac
    29101 actacgaaag atttttgggg acacctacaa cacaagcaaa caacgtaaca accggcagaa
    29161 tctaccaaac cgtaattacc aaagaacgta aaggcgatta tttgggcaaa accgtacagg
    29221 tagtacctca tattaccgat gaaatcaagc gcaggattaa acttttgggc agtaaacacc
    29281 aatatgatgt tgttattacc gaaattggcg ggactgttgg cgacatagaa tctttgcctt
    29341 atgtagaagc tgtacgtcaa ttaagatggg agcttggagc aaataactcg ctggttatcc
    29401 accttaccct ggtgccatac ttagccgctt cgggagagtt gaaaacaaaa cctacacaac
    29461 atagtgtaaa aatgctgctt gaaaacggaa tccagcccga catacttgtt ttgcgcaccg
    29521 aacatgagct ttctccagac ataagaaaaa aagttgcgtt gttctgcaat gttgaaatgg
    29581 atgctgtagt tcaatctatt gatgtatcca ccatttatga ggtgcctctt atgatgcaaa
    29641 aggaaaagct tgatttaact gtgcttagaa aacttgccat gaaagaaggc ggagcccctg
    29701 acatggctgc atggaacagt tttttgcaaa ggtttaaaaa cccaaaatca acggtaaaca
    29761 ttgcccttgt tggaaaatat gtagaattga aagactcgta caaatctatc attgaagcct
    29821 tgtatcatgc tggtgcatac aacgattgta aagtaaacct cgattgtatc cattctgaaa
    29881 aaattacaga acagactatt gataacttat tgcaaggaaa acaaggaata gttgtggctc
    29941 ccggctttgg cgaccgtggt attgaaggaa aaatattggc tgtaaaatat gcccgcgaaa
    30001 aaaatatacc atttttaggg atttgcttag gtatgcaatg tgctgttatt gaatttgcaa
    30061 gaaatgtact aggattgaca gatgcccatt caacagaaat gacacgttca acagagcatc
    30121 cagttataga ccttatggaa agccaaaaag gtgtaaccga gaaagggggg accatgcgtt
    30181 tgggggcata tccttgtaag ctaaaaaaag gatcaaaagc atacaaagct tatggcgaaa
    30241 gtcaaatcat ggaaagacac cgccaccgtt ttgaattcaa caacgaatat atagacagct
    30301 ttgaaaaagc cggcatgcaa cctgttggta ttaacccgga cagtaatttg gtggaaatta
    30361 tggaaattcc ttcgcacaaa tggtttgtcg gagttcagtt tcaccccgaa taccaaagta
    30421 cagtgctaaa accacaccct ctgtttgtcg aattcatcaa atttgctaaa gaataaaatc
    30481 gttttatgta ttctcatcaa tatttaaagg actttacagc ctctgtattc aaaaaaatgg
    30541 gttgtagccc tgaccatgca caaattattg cagatgtttt tattgctgcc gagctgcgcg
    30601 gacttccatc gcacggtatg atacgtttga aggattattt ccaattatgg aaggccaatc
    30661 gcatcaatgt aaacccaaac gtaaaaatta tacacgaatc accatcaact gcagtagttg
    30721 atggtgacaa tgctattggt atggtttcgg ctcatcgttc tatgcaggtt gccattgaaa
    30781 aagcacgtca ggccggaacc ggatgggttg ccactcaaaa ctcaaaccac tatggcattg
    30841 caggctacta ttctatgatg gcactggagc atgacatgat tggaatttgc gttaccaatg
    30901 ccaacccgct tgttgctcct acattttcaa cacagcgtat gttaggcact aacccactgg
    30961 ctgttgccat tcctgccggt aaataccctg catttgttgc agactttgcc actactccta
    31021 tagccagggg caagcttgct gtattagaga aaaaaggaga aaaatcacct cttggatacg
    31081 tacaagataa agatggtaag ccttctgacg acccaagtat cctttcaaaa ggaggttcaa
    31141 tgcttacttt agggggcgat tacgaacatg gcagccacaa aggattctgc atggcttcta
    31201 taatagatat tttctcatct gtattatcag gcgccaactt tgggcctttt gtaccgcctt
    31261 cagtagctta tcttcctgtg ttggaagaaa aagtaggggt tggaacaggc cacttcttcg
    31321 gggctatgcg catagatgct ttccaacctg ccgatgcttt caaagctaag atggacaagt
    31381 ggattgaaac atttaaaaat gcaaagccgg ctgtaggaac aaatggagta atcatacccg
    31441 gtgaacctga aagaaagaag gaagaagaac tcaaagtcaa aggtatttcc attgttcctg
    31501 ccattgaaaa agatgttcag gaaatagctg attacttcgg gctcaaattc ggggaataat
    31561 ttattttgaa aacaaataaa gcgtactatt gtccggctaa tattaatagc cttcgtataa
    31621 atcatttgat ttgaaaagga gagtattttt attggtttct tcctgataca gctcctgaaa
    31681 aaaccaaatt gacaaaaaag gccctaaaca tttgtccagg acccttttca ttattttttt
    31741 atttatactc ctaataagca aatagcggat aattgctcat caatttattt accttttgac
    31801 ggacctctgc aattacagac tgattgtcga tgttagtcaa cacgcggtca atcatttcaa
    31861 caatttgagg aattacatcc tccttaacac cacgggtagt caaagcaggt gtgccaaccc
    31921 tgaaacctga agtttggaaa ggagaacgag tatcaaaagg gaccatattt ttgttcagcg
    31981 taatatcagc agcaacaaga gcgttttcag cctgtttgcc attgacatca gaaagcttgg
    32041 tacgtaagtc aatcaacatt gagtggttgt ctgtacctcc tgaaactacc ttgtatccaa
    32101 ggtcagtaaa tgatttagcc ataaccttag cattggcaag catttgctta acgtatgcat
    32161 cgtacgaatc agttaatgct tcgccaaaag caacagcttt agcagcaatc acgtgttcga
    32221 gcggaccacc ctggatgcca gggaatacag ctgaatccag caaagcagac atcatcttaa
    32281 tttcaccttt aggggtagtt aagccccatg ggtttggaaa atcctttcca atcataatca
    32341 taccaccacg agggccacgc aatgttttgt gtgttgtggt tgttacgata tgacagtgcg
    32401 gaaatggatg tgtcagatag cctttggcaa taatacctgc agggtgagca atgtcagcca
    32461 taagtaaagc gccaacttcg tcagcaattt cgcgcatacg cttgaaatcc caatcccgtg
    32521 aataagcaga agcgccagct ataataagtt ttggtttatg ttcgcgggcc aatttggcca
    32581 tttggtcgta gtcaaccctc tctgtttcag gattgaggct atattcaact ggtttgtata
    32641 atatacctga tgagtttaca ggagaaccat gagaaaggtg accgccatga gaaagattaa
    32701 ggccaaggaa tgtgtctccc ggtttcaata cagctaacat aacagctgca ttagcctgag
    32761 ctcctgagtg aggctgtacg tttacccatt cagcgttaaa aagcttctta gccctttcaa
    32821 ttgcagtacc ttcaacttca tcaattacct ggcaaccgcc ataatagcgt ttcccaatat
    32881 aaccttcagc atatttgttg gttaaccatg ttcccatggc ttgcattacc tggtcgctta
    32941 cgaagttttc ggaagcaata agctcaattc catgaagctg tctttgatgt tccttttcaa
    33001 tgaggtcaaa aattaattta tccttttcca ttttaaaaaa atttgatgct gcaaaggtaa
    33061 ttctttcatt ttaatccatt agaattttta aatcaggtaa agcatgaata attaacaagt
    33121 tattcaccaa ataaaaatat ttccacgaac ctaaagttca ttaaggcaca attaaaaaaa
    33181 aattctcaac ttcggggcaa ttttttcaag ctatcattgt tttaaaagaa aactttcatg
    33241 acaataaaaa aatatttctt tttacttgtt ttattaacag ggttacagca attaatcgca
    33301 caggaaaacg gcattctcaa aggcattgta tctgacacgt ctggctatac tgttggtttt
    33361 gccaacataa cacttgaagg cacgaagttt ggcaccatgg ctgaccgttt tggccaattt
    33421 gaaaccaagg tgcctgccgg gaaatattca ttggtgatta cctgtgtggg gtaccagccc
    33481 caaaaaaccg aaatagaaat tattgccaac caggtgacta caataaactt tacgctgaaa
    33541 gaatcaacac aaaagcttga cgaagtaact gttgcaggaa ggagcgaaaa gagtggcaca
    33601 gtacagcgta tagatgccaa agcctttaac gccgtaccta atacaaccgg caatattgaa
    33661 aatgtagtaa aacagatggc tggcgttgct tcaaacaacg agctcagctc gcaatattcg
    33721 gttcgaggag gcagctttga tgaaaacctt gtttatgtta acgacatcga aatattcagg
    33781 ccggtgctta tccgtagcgg gcaacaggaa gggctgagct tcatcaaccc tgatttggta
    33841 agctctatca aattttcttc tggtggattt gatgcttctt atggcgataa gatatcatct
    33901 gtgttagatg ttacttataa aaagcctact gcatttggtg cttcagccag catcagtatg
    33961 cttggtgcca catcacattt tgaaggctcg gcagctgcaa acaaattgcg ttatctggca
    34021 ggtttcaggt ataaaaccac caaatatttt ctgggaggcc ttgatactaa aggggattat
    34081 gaaccaaact ttttagattt ccaaacgaat attggatttg acatcactcc tacccttgaa
    34141 ttcagcgttt taggcaatat ttcgcaaaac cagtatgact ttaagcccga cacccgagag
    34201 attgaatttg gtacatacaa cttaccctta cgtgctaaca tttattatga aggccaggaa
    34261 aaagacaaat acctcaatgg catggcagca ggcacattga actttcaccc gtctgatgac
    34321 atgtatttta agcttattgg ttcggtttac caaaatgacg aatctgaaac attcgattta
    34381 ttgggggaat acaacattag cgaaattgac aattcatctg gacaagatag cagcctcgaa
    34441 atcggggttg gcggcttcct aaaccatgcc cgtaatttct taaaaatcaa catatacaat
    34501 gtcagccata ttggaatggt gcaattagac aagaataaat tcaagtgggg acttacctac
    34561 cagtcagaat cagtaaacga caggcttaac gaatgggaaa ttattgattc ggcaggttat
    34621 atttaccctt ataatattga caacatcaaa acccttacca gtgttaactc taaaaatgac
    34681 attacttcgc accgcctgat gggctacctg caacatacgt ttgaatttga aagcggcaac
    34741 gacaaatttt tcttaagtgc cggagtgcgt acacaatatt ggtcattcag caaacagcag
    34801 acttggagcc cgcgtatttc actctcctat cagcctggct ggaaaaacgg cctgatgatg
    34861 tacatggcat ggggttttta ccaccagcat gctttcttta aagaaatgag aaacgagcaa
    34921 ggcttactaa ataaggaaat taaagcccag caatctgtca attactctgc cggattggat
    34981 tatatgtttt attcctgggc caggcccttt aaattctctg cagaagtata ttacaaacag
    35041 cttgacaagc ttattccata ccgtatagat aatgttcgtt ctatatactc agcattaaac
    35101 atagctaaag gatatgcaac cggtatcgac ttaaggctaa acggcgagtt ggttaaaggt
    35161 acagaatctt gggtaagcgt ttcgctgctg caaacacagg aaaaaattct tgccaattcg
    35221 acaattccaa gttctaacta ttactcacgc cctaccgacc agtttgtcaa catcaatttc
    35281 tactttcagg attacgttcc cagctatcct tcataccgcg tacaccttaa cgtacattat
    35341 ggaagcgagc tgcctgtaac aataccctgg cagaaaagat gggacaaggt acagaaactt
    35401 ctgccatcat acaagcgagt tgacatggga gtttcaaaaa tgctcaaagg tgaagaagtg
    35461 ccagcaaaat atccctggct caacgttttc aaagaaatgt ggcttaccgc tgaaatattc
    35521 aatatcattg ggttaaacaa cacggcatcg ttcatgtgga taaagacatt ctcgaacgat
    35581 cctgacattc cttcaagctt tgccgttcca aactatttgc cgggacgaag gtttaatatt
    35641 aagcttactg caacttttta accagctttt tgttcatttt aaaaataaaa caacatgata
    35701 caacgtattc aaacatttta cctgatagca gctattatct gcctttttgt gaacacttta
    35761 gtaccaatgg cttattttac agaccatgca aacaatactt tttacctcat gcacaatggc
    35821 cttcaagaat tggttaaagg taaaataatc caggtttatt acagcctttc gataacaatc
    35881 cttctgtctg tggcaatagc tatggctgtt tatattatta tcaatttcaa aagacgggca
    35941 ttgcaaatgt tttcctgcaa aattctttgg gttgcaattt ttgcaattca gggcagtata
    36001 ttgtggatat atttcatgat aaaaccacaa tttgtccatg gcaaattaag tttcacagcc
    36061 tttttgcctt tattggccat gatattttcg atactggctt tcagaaacat caaaaaagat
    36121 gacgatttgg taaaatctat tgaccgcttg cgatgaaaac cgggttcctt gtttttatta
    36181 ctattgcgtt aagtatttac attcttggca actggtactt gtttacaagg ttaaggcaat
    36241 cgtggcctga acaatggcaa ggtttttcat tagtaattac tttgttcctt ttttgcgcag
    36301 gatgttttgt tttaagtcgt tttattggcc atacaagata taccggattg tacaacattg
    36361 tttcgtggat aggttatttt tggctggtaa tattacttta ctctgttgta atacttgctg
    36421 tatttgatat tgtaagaata ctaaattgga ttatgccatt tttgcctgat tcaacaagta
    36481 ttcaatataa aaacttgaaa acatttaccc tgttggttac ttgctgtacc ttggttttgg
    36541 ttattttaac aggatatttc aatgcaaaca acacaaaaat ccgtaaaata agcctttcaa
    36601 gtgagaaaat caatgggaag cctgttgtta ttgccttggc atcagatttg cattttggga
    36661 acatggtatg tgacaagaaa attcaaaaac ttgttgactt aatcaacagc tataaccctg
    36721 atatagtgat acttgctggc gatttgatgg acgaagccca tgaatacact ttcaaaaaca
    36781 aagtcggaca gccattaaag cacttaaaag cctcaaaagg tgtttatgca attgcaggca
    36841 atcatgaata ttttgggggc attaaaggtg ctattcaata cataaaatct ttaggtattg
    36901 aattcttgag ggatagtgta gttgaacttg acgatattgt tattgttggg cgagatgaca
    36961 tccacaacct tggcaccagc aataccagta gaatgccctt atcgcaattg attgtagaaa
    37021 aacatcgcaa caagtttata ttagtagctg accatcaacc cataaacttg agtgagtctg
    37081 caaaagctaa agttgatttg caagtttcgg gacatacgca caatggtcaa atgtggccat
    37141 ttatccatct tgtcaacctt gcctttgaaa gggcttacgg gctcttcaaa attgacgaaa
    37201 caaattttta cattacttca ggttttggca cctggggccc tcctattcgt ctgggcagca
    37261 attcagaaat agccattatt acaattaatt gaaatgagaa attacttata aatctctttt
    37321 ctatgtccca ccgaaacaac cgttacaaca attatggtat catctatttc atatataata
    37381 cgataatcgc caattcgaat tctataagca tcttcacctt taagcttttt ataaccaaat
    37441 ggccttggat tattaccaag gtttgaaata gcattcttaa ataacaggta taaccttttt
    37501 atcaagcttc atgatttgct tttgtgcatg tcgctcaatg aaaattgcat atttcatttt
    37561 atagcttctt ctatttcttt aaaagcaagt tcaaaaggta taggctcgct cttatgcatt
    37621 ttagcttttc tatattcttt aatctcttct aattcttcag aatcagctaa aagtttctca
    37681 taaaccttta taggtaactg aacagcaatt ttattacctt ttttatctac tatataattt
    37741 tctctgagca taaaaataat ttgtattgta aaactacata aaatacgttc aaaatacaaa
    37801 atttgttcag aacttcaact taattaagaa tagcatcact ccactttcaa tacttcttta
    37861 aaagcacgtt taaacgactc tttaggaagt gcacccattg ccatttgtgg tgtaccttct
    37921 tttgggacaa ataaaagaga tgggatactc tgtatgccaa ataatgaaga caactcccgt
    37981 tcttcatcag tatttacttt ataaatatca atcttacctt catattcaac tgacaattct
    38041 tcaagaatcg gagctaccat cttacaaggg gcacaccagt tggcataaaa atcgaccata
    38101 caaggcttat cgccttcaaa cttccaatct ttattggttt cgtaattaaa aactttctct
    38161 ttgaaagtcg ctgcatttag atgacttacc atattttcaa attttattaa aagtaaaatt
    38221 cgtaattcat aattcgtaat ttattcataa acaatcataa aagcaaaaag tttataaaca
    38281 caatcaacaa tggcatatcg aagtaaaatt gtacctttgc agcctaaaga aatggcggga
    38341 acattatgaa gcattgcctc gatagaaaga ttttttcagt aatatcagat attgccgaac
    38401 gagaaaacgt tcaatgcttt gttattggcg gatatgtacg cgattgcatc ctgaaaaggc
    38461 catgtaaaga tatcgacatt gttgttgccg gcagtggtat cagaatagca gaacttacag
    38521 ctaaagccct caagcatact accaaagtaa gtgtttttga aagctttggc acagccatga
    38581 tacgccatca tgaccttgaa atagagtttg taggtgcccg aaaagaatcg taccgcagcc
    38641 atagccgcaa acctattgtt gaagatggta ccctcgaaga agaccagttg cggcgggatt
    38701 ttaccatcaa tgctatggcc ataagcctta acaaagaaga ctttggtaca ttcattgacc
    38761 cttttggcgg gcagtctgac ttgcaaaata aaattatcaa aactcccact gacgccgata
    38821 ttacctttag cgatgatccc ttgcggatga tgcgtgccat caggtttgcc acacaactcg
    38881 actttgaaat tgagcctgct accttagcag caattacaaa aaacaaagac cgcattacca
    38941 ttgtctctgc agagcgtatt gccgatgaat taaacaaaat agtgctttcg ccaaaaccgt
    39001 caaaaggttt taaactgctt gacgaaacag ggcttttggc cattatcttc cctgaaatga
    39061 gcaaattgaa aggggtagaa accatcaaag gtaaagctca taaagacaat ttctaccata
    39121 ccattgaggt attggataaa atagctgtaa actctaatga tttatggctt cgttgggctg
    39181 caatacttca cgacattgcc aaacctgctg tcaaaaaata cattgaaggc cagggatgga
    39241 cttttcatgg acatgaattc ctcggacaaa agatgatacc aggcatcttc aagcgtatgc
    39301 gtttaccgct caacgaaaaa atgaaatatg tgcaaaaaat tgtagggctt catttacgtc
    39361 ctatcgttct ggcccaggaa gtagttaccg attcagcagt ccgtcgtttg ctattcgagg
    39421 caggcgacga tattgacgac ctgatgatgt tgtgcgaagc ggatatcact tcaaaaaacg
    39481 aaaaactgca aaagaaatac ctcgacaact ttgccattgt acgccataaa ctgaaggaaa
    39541 tagaagaaaa agatgccatc cgcaacttcc agcctcctat tgacggtacc gaaatatgcg
    39601 atatctttgg gcttagccct tgcaaaacgg ttggagacct caaaaacgcc ataaaagatg
    39661 caatccttga tggcattatc cctaatgacc gtaaagcagc ttatgggctt ttgctcgccg
    39721 aagctgaaaa agcagggtta aagattatta acccactaaa tatttagaaa tttatctcga
    39781 aaatttatct gaaacacatt tcgactccgc tcaatgtgac gtagtttggt catactgagc
    39841 ggagttgaag tatgatacta atgaaattct tataaaattt taaattttca gaaatactca
    39901 acaatcagtc tttgcataca tatccggcca tagccacagc agctttaaca tagctctcat
    39961 ctacatcttc aacttcagca gtcaaccacc tttcagcgct taccaggtca acatcccacg
    40021 ttttaatttt cgggtcgtta tccaatacct tggcaacttt agacttacag cctgcacagg
    40081 caatatttgt tttaagtttt atggtattca ttttatacac tttttataat aaaacaagat
    40141 tttctctgaa atgtttgaat ttactacaaa taaaaaaggg cttttcagcc cttttactat
    40201 attctatact gctttaagct ttcgctcaat atcttcaagc tgtcgtttga acatcttatc
    40261 tgtatctcgc agatttagta ctgttttgta tgcatgaagc acagtagcat ggtctttctt
    40321 gccaatattg gtcccgatgg tagtaagcga gctctttgta aggagtttag agaagtacat
    40381 cgctacctgc cttgccaata caatctcacg tttacgggta ttgacagaaa gctgatccgg
    40441 agtgatattg aaatagttgc acaccacttt tgaaatgtag tcaatcgtga tttctttctt
    40501 tacatttctt gagattttat ccactacttc tttagccact tcaatactga tagttttgtg
    40561 gttcaaggcc tgggctaaag tagaaatcaa agccccttcg agttcgcgga tactgctggt
    40621 tatgttggct gcaatatatt caagcacatc gtccgaaacc tggataccat ccatgtaaca
    40681 cttgttgcga agaattgcta ccctggtttc aaagtcaggg gctttcaaat ctgcagctaa
    40741 cccccatttg aaacgtgaca acaaacgttg ttccatccct tgcaaatcag ccggagcttt
    40801 atccgaagta agaatcaact gtttacctga ctggtgcaaa tgattgaaaa tatggaagaa
    40861 aatatcttgt gttttttctt ttccgcagaa ttcatgtaca tcatcaatga tgagcatatc
    40921 tatcatctgg taataataaa ggaagttatt cctgttgtta ttacgggtag cctctgcaaa
    40981 ctgagtttgg aaaatgtgag cattgacata cagcacgttt ttttcaggaa atttttcctt
    41041 ggtagccaaa ccaatagcat gaacaaggtg ggtcttgccc aaacctgaat caccaaaaat
    41101 catcaaaggg ttaaatgctg taccgcctgg atgatcggcc acagcagaac ctgcagaacg
    41161 agccaacctg ttacattcgc cttcaataaa gttggaaaat gaatagttag gattgagcct
    41221 cggatcaatg tttaacttct tgataccagg caaaacaaat gggttcatca caggctgctc
    41281 ttcagacaaa ggcatattaa ccggtttgtt tttagccact gaagcacttc tgccaggata
    41341 ctttacagtg taaggcttgt tttcttccga atcattgtcc ataacaatgt tgtactcaag
    41401 cattgcatca ttgcccaatt ccctgcgcag cgttttctta aggatatcga tgtacttttc
    41461 ttcaagatat tcataaaaaa acgtactagg aacttggatg gttaagatgt ttttctcaag
    41521 tttcactgga atgattggtt cgaaccatgt cttgaagcta ttttctgaaa cattatcctt
    41581 aattactttc agacaattgc tccataaggt ttgacatgaa ttttgttcca ttttaatcat
    41641 ttgaatatta attagtcagt gttttttggt ttcgtaaagc aattttggtg aaaaaaaagc
    41701 tgaaaaaaaa atcctttttg ccttgattta ttaaaaaaaa agatatccat ttagttttca
    41761 attttttata aaatcaacga attgataaaa attaattaac taatattcaa atatttacaa
    41821 ttgaagaaaa aaaaacaatt caatgtcagt acattacatt ttgttgaatt atttattttt
    41881 ttttccaaat actgaaaaat gaatatatct gttgggattt tctttgatat ttgtaataaa
    41941 agaatcaaga ttgttagtga catttaacag gctgttgtac aacgaatcct gatataacaa
    42001 gcgactaaca gtcccacttc cgccttttat atctcgaata agattgcgag actctgtcag
    42061 cattgaatcc aacaacatca accggggctc tatcctgatt tttttgatac tatcactaat
    42121 ggccacaaag ttggaaacaa ttttattcac ctgcttatta ttatccttga ggttggctga
    42181 aatagccctg acattttcga gggttagggc tatgttttca ctggatgagg ccaatatttc
    42241 ggacgatacc ctaagatttt taaatgtttc cttcatattc tggatagtct gtttgtcaaa
    42301 tactttgtaa atggcatttg agacagaatc cattgaattc aacaatttat atgcttgtac
    42361 tttgtatggt tcgagctgtt tggcaatggt tgtctctact tctccaatga tggtatcatt
    42421 ttctttacaa aactcgttag acctcgagaa attgagctct actgctttgt ttccgataag
    42481 gtcgaggttg taaatgcggg ctaccgtgtt tttagggatg tttatcgttt ttttgataga
    42541 aaggacaact tttaaatggg caaatgaact gtcagaaaag ctgatactgc ttacatgccc
    42601 cactttatag cctttgatat acaccccatt cgactcaagc aggccgctaa catcttcata
    42661 tatcactata taatctttgg tattgctaaa aaaatttttt ccttttaaaa aattaattcc
    42721 ccagataaga cttccgaggg tagcaaacat gataagccct attttaattt cttttttcac
    42781 agatattgaa cagattagct attaccaaat ataaaaaaat aatggctaat gtacaacaaa
    42841 cagccccagt ttgtttcaag aagtccagac ttatttaact acactttgga gaggaactat
    42901 ctgtccatct ttaacagcca caatgaaagc ttgtggatag tactttttca cctcagaggc
    42961 cagttcaaca gcctgcaaat attgattggt attgcctatg gtatatttat aggtactgtt
    43021 tattctgtat tcagcaatac tattatttag cttcaacttt ttgcaatgtt tgaaaacctg
    43081 gtctttttta ctcacttttt tttgagaagt aaggatttga attttaaaat taacagattc
    43141 atcaacgttt tcgacactta caactgtacc tgaatcagta ttatcctgat aaacaggagt
    43201 cttcaatgca gaatcatcgt tatttttaac ataaaaagtt ttttgttcaa atcttttctt
    43261 atactctcca atagcattgg ctataccatt ggcaatttcc atttgtccag tctcagacga
    43321 aagatacctt tcttcgtctg gattgctgat gaaacctact tctaccaaaa ccccaggcat
    43381 cgctgttttc cacaacacca gaaacccagc ctgacgcaca cctctatcta atgcagaatt
    43441 gtaccgcgac gaattgctct gcaccaatcc agcaaaatca aggctttgta ttaaataggt
    43501 gttttgcatg gaagaaaaca taatatacga ctcggccgaa ttggggtcaa agccttcgta
    43561 gcgggttgtg tagtcttttt caaaaataat ggcggcattt tcctttttag ctacatcaaa
    43621 gttgctttca ttcttgtgca agcccatagc ataagtttcg atacctttta tgtctttgtt
    43681 tttattggca ttggcatgta tcgaaataaa aagttcggca ttatggcggt tggcgatttc
    43741 ggccctttcg tgcaatggaa caaaaacatc agtattgcgg gtataaatta catctacatc
    43801 aggaaatctt tgcgaaatga gctttccaac ttttaaagca acagccaaca ccacatcttt
    43861 ttctttgtga tttttgccca aagccccggg atcttggcct ccatgacctg gatcaattac
    43921 aatttttgtt aaagaattct ttttttcgtt tccagatttt gtttgttcgt caacggcgta
    43981 agtttttgac atacaaatac atgaaagcac aaccaacaca accgaaaact tgtaaacaat
    44041 tccctgataa atatttttat acatcttcca atgcaaaatt ttaattagct ttgagcgttt
    44101 tttttgcaaa aatatacaaa gttggaaatt aggtatataa aaataaaatt gggaaggttc
    44161 tttttattta catataagct ttgcttttta ctattgtttt tggctgtaaa tacggctaat
    44221 gcacaggttg acactactaa ctccgtccaa ccctttgaca atttaataaa aaaagattct
    44281 actttaaatg atactactgc taatgctgcc attgcaaatg acagtgttaa aaaaaaaggc
    44341 agcgaccttg acgaaccggt aaagtactct gccaaagact ccatgtttat tgacatggaa
    44401 aataaaacag ttcatttata cggcgatgcc aaagttacct ataaagatat cgagctaaaa
    44461 gctggctaca ttagcctggc tactgaagac cagatagtat atgctacggg aactattgac
    44521 agctccggga acatgagcaa ttacccaaca tttaaagacg gaaaagaaga atttcagtcc
    44581 cactggatgc ggtacaattt caaaaccaaa aaaggctttg tttactttgt aaaaacaaag
    44641 cagggcgaag gaaccctcat aggcgactca accaagcgtt cggccagcgg cagtgtgagc
    44701 ttgaaagggg caacctattc gacctgcgac ctggaccatc cccactttta catgaaactc
    44761 acaaaagcaa gggtagtacc caacgataaa attgtttcgg gcccggcata ccttgttata
    44821 gctgacattc cattttacat gctttttgtg ccttttggct atatccctaa tacaccagat
    44881 ttttcgtcag gtttgctatt gcctacctgg ggagaggaca aagctaaagg ctttttcttc
    44941 agggatggag gttattattt tgccatcagc gattacatgg atgttacatt aagaggtgaa
    45001 tattacacct atggctcttg gggtgcaagt ctgagtacac gctataaaaa gagatacgga
    45061 tatactggaa acctcaattt tacttttgcc agaaacatct atggagaaaa agggtttcct
    45121 gattatcaga cttctaatga ttacaaattc atttggagcc atcagcaaga tagaaaattt
    45181 aatcctaact ccactttttc ttcaaacctg aacattagtt cgtcaaaata tgacaggcac
    45241 aactcttacg atccggctga acatacaagg aacacaaaga gttcgagcat aagctattca
    45301 tacaaatggg ctaatttgcc ttttagtatg accacttcgt taaaccattc gcaaagtagc
    45361 cagactggta aagtagattt aacattgcct caagcctcat taacaatgga aactctttat
    45421 ccgttgagaa aagaagaagg agctgccaag cccaaatggt acgaaaacct ttctattcgt
    45481 tatagttcaa aattagataa caaagtttat accaacgatt cagcctttct gactgaccgt
    45541 atgtgggacc gcatgaaaaa tggatttagc catgacattc ctattgagtt agttataaaa
    45601 gtgaccaaat caataaatgt cactccaatt ttaaaataca caggggttgc ttatacagaa
    45661 tatgatacat atcaatggaa tgacaataca accaatccaa gaaccagaag aaacggggct
    45721 gtggacacca tcacccatca tggcttgact tatgcccatg ctctataccc ttcagtttca
    45781 acaggttatt cgcccaagct ttttggtatg taccaatttc gcaactcaag ggttaaagct
    45841 ttaaggcatg tgatgtcgcc ctctgtatcg gtatcatata ccccggatgt aagtgatttg
    45901 ctccccaatt actactataa atataagtcc gatacaaaca gatatacaga aacatcatgg
    45961 tatgccggaa atatatacgg cacccccacc catcctcccg gaaaaccagt tgcttctgtt
    46021 aattttgctt taaaaaacaa ttttgaaatg aaaattgccc cttcggcaaa cgacacctcc
    46081 gctaaaaacc gtaaaatatc gttgcttgat aattttgatt ttagtggttc atacgacctt
    46141 attgcagatt cttttaacct ttcaaatatc acaatgcaaa ccgggacttc actttttgac
    46201 agtaaattaa aaattgatat tagaggcata tttaatccat ataaaactga ctctaccgga
    46261 aacagggtgg tcaactccta tattctaaac gattgggaac agccagcccg attgacagat
    46321 ttatcactgt ccacaagtat atcttttagt tcatctgagc taaaatcaaa aaaagaagac
    46381 gggaaaaaga ctgaacagac aacagaagaa ggtgatgaca actcaattcc ctggaacctc
    46441 aatattggat atagcctcac ctatgagcat ataaaccctt ataaaaaata tagcattata
    46501 cattcttgca cattttcagg ctcattgaca ttgacaaaaa actggaaact tggtattcaa
    46561 tcaggttatg actttaccaa caaagagttc aatatgacat ctataaacat cacccgtgat
    46621 ttgcattgct ggcaaatgac attcaatgta ataccatttg gttctatgca aagctacagc
    46681 ttcaacataa gtgcaaaagc agctatcctg caagatttga aatactcaaa ggtcaaccaa
    46741 tcctggagag gaaggtatta gaagagagtc aagaatcaag aacaaaagat gctctttttt
    46801 ctatgaacta acaactaata actaactaaa aattaagttt tgacttgagg tttttgtagc
    46861 ctgaatcgct gaccttaagt ttggcaccac ttttcaacac aaccacatag ctatccttag
    46921 tgaaatgctc taactggata atctgctcaa catttactat gtaagaacgg tgaatgcgga
    46981 taaacttatc gccatccagg tgtgattcaa agtatttcat ggttttctcc ttcagatact
    47041 tgccttcatc ggcataaatc attacataat catcctgggc ctcaatataa tgaaccgaag
    47101 gacatggaat aacctttatt ttagtaccat ttttcacaac aatacggtct ataatagctt
    47161 ccttctcatc catgctattt agcacgccct gaacctctgt ttcagcctgt ttggtgtttt
    47221 ttagcttttc aatagcttta tttaatgcca cttcaaaacg ctcggccgaa aaaggcttca
    47281 acagataatc cacagcattc atttcaaaag ccttgatagc aaactggtca aaggcagtgg
    47341 caaaaatcac cacaggcgta aactcaacca gctcaaggat ttcaaagccg gtaagcttgg
    47401 gcaattgaat gtcaagcatc acaatgtcag gtttcatctg gttaatggct ttaacggcat
    47461 taaacccatc cgctgcttct ccaataatct caatattaga gaacttcttg aaatagcttt
    47521 ttaccaattc cctggctggc tcttcgtctt caactaataa caccctgatg ttgctcatat
    47581 aaattaggtt cgttaatgat ttgctgcgga attaatattt gaacttcaaa tacttcgtct
    47641 gttttagaaa agttcatcag tccttcggtc atgtaggtaa ggcgaagccg ttccttgata
    47701 tttttaaggc caatacctgc gccttttctg gaaggtactt cgctgtcata attgtttctt
    47761 actttaatta ccaggtaatt gtcattcatt acagcctcaa tttcgagggt tatagtgttg
    47821 acactttcat aaacaccatg tttgatggca ttttcaaaaa gtggctgtaa tatcattacc
    47881 ggcaatgttg ccttataaca atcttcgtta atatttgtcc ggtaattgag ccgacttcca
    47941 aagcgtatct tttcaatatc caggtatcga agcatattgt ccagctcggc ttgcaaggtt
    48001 gtcagctggg tagagttata cttaatgcta taacgcaggt aatctgagag tttgataatc
    48061 atctcctgcg cttttgcagg attggtgatg gttagcgaac tgatagaatt caggctgttg
    48121 aacagaaagt gagggtttat ctgcgatttg agcatatcca gctcagtttg cttaatgatg
    48181 gaaagaagcc tttcttcgtt ttccttgcgt tgtttcaggt aataattgta aacaatgttg
    48241 taataaacaa gcaccgctgt tatgtaaaaa atcaaacagg taaaaacaaa gcctttgata
    48301 tgttgctgta taaaattggc atcataaggg tttaaaagga ctttattaaa tagcgtgaca
    48361 gaccccacca aaaagaggat aataaccgat gccgaaatac agtggttgat aatgacattg
    48421 gaaatatcaa ttttttcggg ataactgtaa tagaccgcat accaaatagt taaacccata
    48481 actgcaaaaa acaggtttac cataatgctg tttaatatgc tgccttctaa agataaatga
    48541 tgtttgaaat aaagaaaaaa gaaactaata acggcaaaaa ctaaccatgt aataagatag
    48601 gcaatagttt tcctgaaact ttccagtatt ggatggttca tggcttaaga cttaccgtgt
    48661 tttgatttca ccacctccga aaatagctac gccacgtata atcaactcgc tatcagagtt
    48721 tatagaaatt ttggccacct ggcgtttgtc tgcaaaacct cccaatatag aagaaacttc
    48781 aatacggacg cgccattcgg caggtacaat aagggtaacg ccaccaaaga tacattgtag
    48841 ttccatcaca cttttacctg gggccaatgc tgcattggta aggtcaagtt ctgcaccacc
    48901 aaagatagaa gtaacacggc ctccacaaaa attttggctg ttgatattgc gttttatccc
    48961 gctaaaaaca tttacatcat ccaccaaatc agatgctgta acttcttctc tgaaagaatc
    49021 gcaaaacctt ttggcatccg gctttcgcct aaaaataata acaatgccaa taaatataag
    49081 tattaccggc caaaacaacc gggtaaaatt aaaaggaaca ttaaaaatat acggaagtag
    49141 aaaaaaaact ccaatagaaa tcaatataag gcctggaaca gccccatgac cggaaacagc
    49201 tattaaacca atggctataa gcaacatttg ccacgaaaag aaaatatgct tgatgttttc
    49261 tggcaaaatt cctatattgt caaacaaaaa aagcacacct gcacacaata caataatacc
    49321 aaacattaac tttttgcttc ttggtgcatc tacaggtgta atatgatgat gttcgtgtct
    49381 catattgtaa tttttctcca aagataagag tgttatcaaa ccagacacag aaaatttcgg
    49441 taaatattaa ttattcatcg gtaaaaatgc catttgtgta tttatgacat ttctgataat
    49501 tggttgaata gcatttccct gctattccca atgtagaatt tcgaaacctt gtaaggtcaa
    49561 ccattaacaa gtataatgaa aaaactgact ttgcatatta ttaaatcatg caatgctttt
    49621 gaactatcag gcagcgttat attctatctt tttaatcttt tctaattcag attgtttcgc
    49681 tctttttgct tcctctaaat caaaatcatc ctgtaatcct aaccaaaatt ttgcagaatt
    49741 accaaaataa atacttaacc ttaatgctgt atcagcagta attcctctct tcccttttaa
    49801 tatagcagat gtgcgggtct gaggtatccc aatatctttt gacaatttat aggcagaaat
    49861 attcattgga attaaaaatt cctccattaa aatttctcct ggatgaatgt ttgctagtgt
    49921 tttcataatc aattaattaa tgataatcta caattttaac attttctgat tgtccctcaa
    49981 cccactgaaa aattattctc cattggtcat ttatcctaat gctataataa tcattaagtt
    50041 tgccacctaa tttttccaat ctatttgatg gcggaatctt caaatcatta atggtttgtg
    50101 agttatttag catcctgagt tttctgcgac ctatagtttg aatctctaaa ggaagttgct
    50161 taaccctaat cccattataa atctgttctg tttccttatc tccaaacgaa agaatcataa
    50221 ttacgacaat cgttacttac gacaaaagta agtataattg ttgtataaaa aaaatttata
    50281 atgaaattat acatctgtct gattataagt atattaacaa agtgtctatt atgccgttta
    50341 gcactaaaac catataaaag gaccgcaaag aagatttctc aactttgcgg tcctcaatat
    50401 ttgtaattta tactctctgc tatttttatt gcttaaaagt gtcaatcaat acacttattt
    50461 cagcataatg cttgaagtca gcttaatatc ttttgatgaa ctcccgacca taaatgtaaa
    50521 ttcgcctggt tcaaccttcc aaccgttggt attcacatcc caaaaagcaa agtcgcgttc
    50581 tttgaccttc atgctaacag tcttggtttc accagctttc aatgataccc gtgaaaagcc
    50641 ttttagttct tttacaggac gggcaacaga gcattctttg tcagacaaat aaagctgtac
    50701 cacttcatca ccacttcttg ccccggtatt ggtaacatct acagaaactt catacgtgtt
    50761 ctttgccaat tctttaactt tcaggttact ataagcaaaa gaggtgtatg aaagcccgaa
    50821 tccaaatgga taagctggtt ccatgttgtt tttttctaaa aatttataac caacaaaaac
    50881 gccttctttg tactcaactt tcaaagactt tttgtatcct tcaaaagccg gagattgctt
    50941 agcatttgca atgtatgtaa atggcagctt gcccgaaggg ttgacatcgc ccattaatac
    51001 tgaagcaata gcattgcctg tttcttgtcc gataaaatac atattgacaa gtgcttttac
    51061 ctctttaatc catgaagaca cgtttatcgg acatccgctg tttacaacca caatagtatt
    51121 tttgttagct ttagccacag cttttacaag ggcttcttgt ttacctggaa gttctaaccc
    51181 atcacggtct cgtccttcgc tttcaaggtt agagtttgtg ccggcaaaaa caatagctac
    51241 atcagccgaa gcagcaactt ttgcagcatt ttcaatcggg tcgccagctt tctcgttgat
    51301 acgtacaagg cctaactgaa taacagcacc accgccatct tcaaaatact caagtttgat
    51361 tttatagctc tttcctgctt caagtgtggt ttcgtattgg tcaacgatag gcccgtggtt
    51421 gttccagttt tcaataactt ttttatcatt caaaaacaaa cgcactccat catcagaaag
    51481 tgtctgaaga ttatacttgc cagtttcttt gacttttaat tcgcctgtcc agcgacagga
    51541 aaagaagttt ggcttaatgt ttggggctgg tgaacctgta ccataatcga aatttacttg
    51601 tttgtcaagc ctttccaaaa caggtttacc ttgcaactct ttattcccgt aatattcagc
    51661 ccaaagtccg ctggcatttt tactactgca aaacaaaaac gagccatcaa caggttcaat
    51721 cttaggctgc ggtattgaat cgccggtaga ataaacaatc ttaaccgaat cgccaagcct
    51781 gcatttgagc ccctgtaatg gggaaatatc ataataaggc gaaataaacg agcttccacc
    51841 accgcctgta gctgtataag cagcattagg gccaataact gcaatagttt taactttcga
    51901 aacgctcaga ggaagaatat ttccttcatt cttcaaaaga gtaatagctt cctgtgcact
    51961 ttttaaaaca agttttttat gggcatcacc atgcaataca gacgtatcag ctttttcatt
    52021 attgtcaaac aagccagccc agaaagctgc tgttaaaatg cgacgaactt tatcatctat
    52081 ggtactttct ttaatttgtc cagattgaat atacttcaaa atactgtctt tattgaaata
    52141 aacgcctaca ggcatttcca aatccagacc agcattggca gcattgacag tgctataaac
    52201 agatacccag tcagagatga caaatccctt gaagccccat tgtcctttaa ggatatcgga
    52261 tatcaggtga taattttctg tacaatgctg gccattttgc aggttgtatg cggccatcac
    52321 gcttttcacg tcagcttctt taacagcagc ctcaaaaggg gcaaggtggg tttcgtgtaa
    52381 tgcacggtca tccaccttta cgtcataatt gttccgttcc cactcctgat cattggtagc
    52441 aaagtgtttt acagaagtaa taaccttttg gctttgcacg cccaagatcc agtttaccgc
    52501 catacgtgat gtcaggtatg ggtcttcaga ataagattcg aaatttcggc cattcaacgg
    52561 gaaacgatga atgccaacac atgggcctaa caggtaatta cggcctttag ctttggtttc
    52621 tttacccatg gcaatgccaa tctcttttac caacgcagta tcccatgtag cccccattgc
    52681 tacaccagaa ataaatgacg ttgcctgtcc attccttacg cctaaagggc catcggtaat
    52741 acgtattgcc ggaataccaa gtctttcaat ggcaggagta tcaaagcctg tggtatcacc
    52801 agaaataatg gcaatttttt cttcaagtgt catctttgcc agcaaatcat tgacgcgcgc
    52861 ttcaacagat tgtgaagggt cctggtatat tgctttctcc ttacacgaaa gaagaactaa
    52921 agcaaaacca agaactagga tttttaaata aatctttttc ataactcttt taaatggttg
    52981 ataatttgta attaggttgt ctgtaatatg cgaataaaag tatttctaaa caattataca
    53041 agtttttaaa tacatgttaa tgtacaaaat attgcttaaa tatcttaatt ttaatcactg
    53101 ttgtccggta aaataaaata aatacttata aatactagta atatcgttga ttagaaattg
    53161 gtcgccccgc tggggcttaa ttttaacgat gtttaatttt actacaaaca ggacgtcgct
    53221 acgcgactaa ataatagcag cgtagctgcg aactgtttgt aggataacca aataatatta
    53281 ctaagccgca tagctgcgaa ctgtttgtac cggacaccaa tgaattttaa tatgaataat
    53341 aaaatatggg catactgtta aataagcatt ccaaagttat cgtgcaggga tttaccggca
    53401 cagaaggctc atttcatgct caacagatga tagaatatgg cacccaggtt gtaggagggg
    53461 ttacacccgg caaaggaggt tcattttatc tcaacaggcc tgtctttaac actgtttatg
    53521 aagcaaaaaa agctaccggt gctgacacgt cagtaatatt tgttcctccg gcttttgcag
    53581 ccgatgctat catggagtct gccgaagaag gaatagctct aatagttgct ataacagaag
    53641 gaatcccaac ttcagacatg gtgaaagtaa aatcatacct ctccgacaag aattcaagac
    53701 tgattggccc caattgtcct ggaattatta ctcccggtga agctaaggtt ggaattatgc
    53761 cagggtttat tcactcccct ggcaatatag gcattgtttc ccgctcaggg acactcactt
    53821 atgagtcagt atttcaaatt tcgcaattag ggcttggcca atccacctgc gtaggcattg
    53881 gaggcgaccc tgtaattggt accacgacat tagaggttgt aaaattgttt atgaaagacc
    53941 ctcagaccga aggcatcatc atcattggcg aaataggcgg aaatatggaa acagaagccg
    54001 ctcaatggat aaaagacaac agctccaagc cggttgttgg gtttattgcc ggacaaaccg
    54061 caccaaaagg aagaaggatg ggtcatgctg gcgctattat cggagggata aacgataccg
    54121 ctgaagcaaa aatgaaaatc atgcgagaat gcggcattta tgtagttgac tctcctgcaa
    54181 acattggcag ggatatgttt aaagctttaa aaaaatttac agcatagatt gcctggcaaa
    54241 ttcagaacat attatggcag tcgcaacagc tacatttaat gattcgcttc ctgcacctcc
    54301 cacacgaaat gaaggtatgg taatggattg cgtaacgtaa cttccaattt tgtccgaaat
    54361 gccttttgac tcgttaccca taacaattat ggcctgtttg gccaacgaag cattgtaaat
    54421 attttcacct ttcaaaaatg ccccataaac agggacgtta gcatttcggg cctcttgcaa
    54481 atagctttct aaattggtat aatgcacctt gaccctcaaa aaagccccca tcgttgattg
    54541 tatgacctta ggattataaa aatccgcaca atcattggaa cacacgatgt ggtcgatacc
    54601 aaaccagtca gccaggcgga tgatggtacc caggttgcca gggtcctgaa tacaatccaa
    54661 ggctattatt ctttcgtttt tagaaggtaa aacggccttt gcatgagcaa ataaaggtaa
    54721 aacagccatt accctgttcg gggtaacctg ccccgaaagt ttttttaaga ctgcctcatc
    54781 tacagcataa caggtgtacc tttgaacagc acgcttatct atttcctgca accaacctgc
    54841 ctcagctata atataatcta tctttaagcc agacaccaac aattctctga caagtttatc
    54901 gccagagaca ataaactgct tatattcttt tctgaatttt ttgtcatgaa gctgactaag
    54961 ccttttatac aatgcattgc tgagcggtac aaaagaaaaa gcgttattca tcggcctgat
    55021 ttttttgacg cacaaaatta aacattaact gatataaaat actttcgtta aaataatgaa
    55081 ttccatgctt cacatttaca tctgcaaata atctcttaac aaggttgaat agaacccgca
    55141 tgaatttcaa tttgtcggga caaattctcc tgcttttgca atacaaacca tccgtcctta
    55201 aaaaatattt ttgttctcat gaaacgagca tgatttttt
//
LOCUS       DAOSPF010000015        53340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_661_length_53340_cov_7.404804, whole genome shotgun sequence.
ACCESSION   DAOSPF010000015 DAOSPF010000000
VERSION     DAOSPF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 53340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_53340_cov_7.404804"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1752)
                     /locus_tag="PK252_04700"
     CDS             complement(214..1752)
                     /locus_tag="PK252_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14042.1"
                     /translation="MNLTVSKKIKLGFSIILLLILINLTFTVFISYRNEILKRETTRV
                     LEPISAALNELYSLVEGSEILTKSWVYIEKFDNTPNKGKLRKIHQQTFFSTDSTIKAL
                     LYKWDSPKANYVKENYEKISQEIKDTLFRTQNMIMTRLSKMADYEDPIAMMSVSPLVD
                     QNGLLSVQSRKALDDIAMVQTIINAKLKAMQKETNDVYSAFQVYTIISGLIVIIISVF
                     VSFWIVKSVVNPLKEAVTFAKHIENGELNTSIKIKSHDEIGELSIALQNMQQTLIHVI
                     GLIRDGSEQLTQASATLNDKVCTMSDNAQNQAASAEEISASIEEISTNVESNMLHLEK
                     VKDIALHATSEVKAVNTLSKISAESISKIDNYTSMISDLAFQTNILSLNAAVEAARAQ
                     EHGKGFSVVANEVRKLAERSKITANEINDLTKAGLNQTTNASNKLQEIMPEIEKTAQL
                     IEEITYSSAEQTTNINQITNAVLVLNKKIQENASSAEFIASHTNKLETLATELKEIVA
                     YFKI"
     gene            complement(1786..2304)
                     /locus_tag="PK252_04705"
     CDS             complement(1786..2304)
                     /locus_tag="PK252_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOT14043.1"
                     /translation="MMNLLRISIAVLCAVNFLIGTSAQVKSVKIGNQVWMAKNLDLEI
                     AGSFPYANAQSTAMYGRLYTYQAAMKACPAGWRLPTESDWDELISQLGGEDVAAGKLK
                     ADGASGFNALYGGYANGSSYMFVGTFGGFWSATSSDTEHAWYYFFTNKSNMLTKSFFN
                     KNYAFSVRCIKK"
     gene            2937..3461
                     /locus_tag="PK252_04710"
     CDS             2937..3461
                     /locus_tag="PK252_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT14044.1"
                     /translation="MALIKSVRGFTPKIGNNVYLADNATIIGEVEIGDDCSIWFNTVI
                     RGDVNGIKIGNKVNIQDGSVLHCLYEKSTIEIGNNVSIGHNVVIHGAKLCNNVLIGMG
                     AIVMDHAVVGENSIIAAGSVVLDSTIIEPDSIYAGVPAKFVKKVDPKQSAEMIQRIAN
                     NYLMYASWYKEGKE"
     gene            complement(3701..6067)
                     /locus_tag="PK252_04715"
     CDS             complement(3701..6067)
                     /locus_tag="PK252_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14045.1"
                     /translation="MKNILYISVLLFGSNILIAQKSNGVVQGTVYDASNNMPIEFASV
                     AIYGTNFGSITDDKGNFTFNNVPPGFIEIRVTALGFEPYISEQLRVTNAGKTFVEVKM
                     VEATQQIEEVVVKASPFRRDKESPVSLRNIGLKEIEKSPGGNRDISRVIQSFPGVAST
                     PNYRNDVVVRGGGASENRFYLDGIEIPNLNHFATQGSSGGAVGIINADLIREVSFYSG
                     AFPADKGNALSSILDIKQIEGNSEKVKVKSGIGASDFALTLDGPIGENTNFILSARRS
                     YLQFLFSVLELPFLPIYNDYQIKVKTRLDNKNELTFMSIGAYDVSDLNLEANKTEKQK
                     YILSYLPAYSQWNYTIGATYKHFHEHSYDTWVVSRSMLNNNQIKYANNIELPENKLLD
                     YNSFETENKFRYEYNRANLLGLKVIAGAGFEYARYYNSTYRKLFGGEDLYKSDFDLFK
                     WNFFGQASKELLDKKLTISFGLRSDANNYTSQMKNFVNQFSPRLSVSYALTQSLTLNS
                     SVGRYYQLPSYTTLGYRDTLGVLINKFNNLKYMQADHIVAGFDILPTSNSKFSIEGFY
                     KNYSNYPFSIRDGIALAGKTVDYGTYGDEPVTSTGVGRAYGAELLYQNRDLMGANVML
                     SYTLVRSEFKTAYGGYSPSAWDNKHLLNILVAYEFRNNWTVGSKFRLVGGAPYTPVDI
                     QRSSLVEAWDVNNQAYLDYSRFNSLRLKAFHQMDIRVDKEFFFNRWSLTAYMDIQNVY
                     NFKSDSPPTYLVDRSVPIIPNPDRYTLKTLENDGGGTILPTVGVIIEF"
     gene            complement(6198..6926)
                     /gene="lptB"
                     /locus_tag="PK252_04720"
     CDS             complement(6198..6926)
                     /gene="lptB"
                     /locus_tag="PK252_04720"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOT14046.1"
                     /translation="MILRTENLVKRYKSRTVVKSASFQVEKGEIVGLLGPNGAGKTTS
                     FYMIVGLITPNEGKIFLDDEEITHEPMYRRAQKGIGYLAQEASVFRQLSVEDNLRAVL
                     EMTKLSKDEQNERVESLLTEFSLHKIRKSLGIQLSGGERRRTEIARALAINPSFILLD
                     EPFAGVDPIAVEDIQEIVKKLKQKNIGVIITDHNVHETLSITDRAYLLYEGEILKSGT
                     AEDLANDEQVRRVYLGKNFELRTV"
     gene            complement(7026..7832)
                     /gene="tatC"
                     /locus_tag="PK252_04725"
     CDS             complement(7026..7832)
                     /gene="tatC"
                     /locus_tag="PK252_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT14047.1"
                     /translation="MKPDGEMTFLQHLEELRWVIIKALIGIVIGAALVFFFKNIVFDV
                     ILLGPRSPGFISNRLLCRVGGYLGTTSLCINSHPAPLQNITMGGQFNMHIWVAIVGGF
                     ILAFPYVFYQFWSFVAPALREKERKAARGAVVVSSLLFMTGIMFGYFILLPFSIDFLT
                     TYSVSDLVENHINFVSYISNVTSVVLACGLVFELPVVVYFLSSIGLVTPALMKKFWRH
                     AIVVILIIAAIITPPDWVSQVLVAFPLFALYMGSISISAWVVRKRERAGS"
     gene            8173..10359
                     /gene="recQ"
                     /locus_tag="PK252_04730"
     CDS             8173..10359
                     /gene="recQ"
                     /locus_tag="PK252_04730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOT14048.1"
                     /translation="MIKEDLSLQDYLKKYFGFTSFKGNQEAIIRNLLDGNDSFVLMPT
                     GGGKSLCYQLPALIMEGTAIVISPLIALMKNQVDAMRTFSEEDGVAHFLNSSLNKTQI
                     NKVKEDVLSQRTKLLYVAPESLTKEENVAFLKQIKVSFYAVDEAHCISEWGHDFRPEY
                     RRIRPIINQIGHAPIIALTATATPKVQNDIQKNLEMLDAKVFKSSFNRANLYYEVRPK
                     IDPAKQIIKFIKSKGSKSGIIYCLSRKKVEELTELLKVNGIRALPYHAGMDAATRATN
                     QDKFLMEEAEVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRAGRDGGEGHC
                     LTFYKYQDIQKLEKFMQGKPIAEQEIGNQLLQETVSYAESSVCRRKFLLYYFGEVYEK
                     DNCENCDNCLHPKTQFEGQEYIEQVLECILAVKEKFKGEHIANILSGNLTTAIKSYKH
                     NKLELFGVGADRDIKFWNGIIRQMLVNQFIAKEIENYGLLKVTEKGLQYLEKPYSIMF
                     TEDHDYEVENEEDQVVMVGKTTTVDEELLHILKDLRKKIAKQKNLPPFVIFQDPSLED
                     MAIQYPISVEELQNIAGVGAGKAMRYGKEFVELIKSYVEEKDIIRPQDMVVKSVVNKS
                     GLKVHIIQSIDRKMNLEDIAASKNLSLQELITEIEAIVNSGTKLNIDYYINEILDEDH
                     QQEIYDYFREAETESIEDALSELGTDEYSEEEVRLMRIKFMSEMAN"
     gene            complement(10356..10961)
                     /locus_tag="PK252_04735"
     CDS             complement(10356..10961)
                     /locus_tag="PK252_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOT14049.1"
                     /translation="MNFLAHIYLSGNSNEIRLGNFIADYVKGAEHTRYPTLVQRGILL
                     HRKIDYFTDTHPIVKRHKLLFQPKYRKHAGVVTDIIYDHFLASEWHHFSDIPFDDYVA
                     QVHDWLNDYMESIPLEMRRIMPGFIKNSWLQSYRTLEGIESVLIGMSKGTSMPPENAF
                     AIYVMHKNYQSLKKDFFAYFELLCEYSQKELDRMEMEGNRS"
     gene            11093..12430
                     /locus_tag="PK252_04740"
     CDS             11093..12430
                     /locus_tag="PK252_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOT14050.1"
                     /translation="MRKRIIIIGAAGRDFHNFNMLFRDNELTEIVGFTAAQIPDIDGR
                     KYPAELAGKLYPDGIPIFAEKELPMLIKTLKIDECLFSYSDVSYSKIMSLASVVNAAG
                     AHFTVAGTTNTMLKSNKPVIAVGAVRTGCGKSQTSRKIAEIINQKGIRTIAIRHPMPY
                     GDLVKQKVQRFASIEDLQKHECTVEEMEEYEPHIMRGIPIYAGVDYEAILRQAENDPK
                     GCDVILWDGGNNDFPFINPDLMVTVLDPLRPGHEVAYYPGETTLRIANIAIINKIDSS
                     TPENVELVKNNIAKVNPNAIVIEANSPISVENANIIKNKRVLVIEDGPTLTHGEMKIG
                     AGIVAAEKFGASEIIDPRPYLVGKLKDTFQIYPNIGKLLPAMGYGQQQLADLEQTINN
                     TVCDAVIVGTPINLGRIININKPYTRVSYELQEISHPDLETLLNEFLKEKHFY"
     gene            12462..14000
                     /locus_tag="PK252_04745"
     CDS             12462..14000
                     /locus_tag="PK252_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT14051.1"
                     /translation="MLVKTFGSAVHGINATTVTIEVDVSQGINFCIVGLPDSAVKESQ
                     QRIDSALRANDYKMPGRKVTINMAPADIRKEGSAYDLPLAIGILAASDQITSDKLDKY
                     VIMGELSLDGGLQPIKGVLPIAIKAREEGFKGFILPKQNAREAAVVNNLDVYGVETLR
                     EVIDFMTGQKELDPMEVNTREEFYANVSNYDVDFADVKGQENVKRALEVAAAGGHNII
                     LIGPPGAGKTMLAKRIPTILPPLTLHEALETTKIHSVAGKMDRNSSLMTVRPYRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRAVLEVMRQPLEDRIVSVSRAK
                     FSVEYPASFMLVASMNPCPCGYYNHPEKECMCSPGIVQKYLNRISGPLLDRIDIHLEV
                     VPVPFEKLSEKRANETSSQIRDRVTTARKRQEERFAGQKGTHCNAQMSSKQLRKYCEL
                     DSASQELLKNAMQRLGLSARAYDRILKVSRTIADLEGKENIESHHLAEAIQYRSLDRD
                     GWAG"
     gene            14021..14353
                     /locus_tag="PK252_04750"
     CDS             14021..14353
                     /locus_tag="PK252_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14052.1"
                     /translation="MKLKITLFTFFVFLHLMFIGQKQSVRIGPPCPKQQYEPLFVIDG
                     IRIPPSVGSKFMDEKKETIDSLSLQPDSIFDCNGKVTNFGIVRIFTKDSINTGAKKIQ
                     FLTDYYLN"
     gene            14534..15481
                     /locus_tag="PK252_04755"
     CDS             14534..15481
                     /locus_tag="PK252_04755"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOT14053.1"
                     /translation="MKPRLIIIAIALVAICKTSFSQVTEAEDKLKVQTKDTLQGWRTG
                     GVLGLSLAQTSLTNWSAGGQNSMAGNGILSFFANYKKDKTAWDNSFDFGYGLLKQGTD
                     KDYKKTDDKIDILSKYGHEAFKNFYYAALFNFKTQMTAGYKYPTDTTKVKISDLFAPA
                     YVVGALGMDYKPNAYLSVFAAPLTAKMTIVNNQALADAGAFGMKDGKKSKTEFGGYVR
                     FIYSKNDFKNEALKNVSFTTKLDLFSNYAEDPQNIDVSWETQIAFKVNKYISVNVNTH
                     LLYDDNIKTIEKDGIGQDVVKGAKIQFKEILGAGFSYNF"
     gene            complement(15500..22834)
                     /gene="sprA"
                     /locus_tag="PK252_04760"
     CDS             complement(15500..22834)
                     /gene="sprA"
                     /locus_tag="PK252_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOT14054.1"
                     /translation="MGTGLTKFSKYLFTAITLVFIGAALMTTEASFRVFKKTPSQYED
                     DEEPVDDTNFKAEGPQFKLKKDPDHVNSEIGEPEGIQLKNPSNFKSKVEYDPETNQYV
                     VKNKVGNLDYRQPYYMEPSEYRKYEMQKTVRDYWKQQANGGKGTGRNLGSSFNVGGEV
                     FQKVFGGSTISIVPQGEAELIFKGNVNTTNNPIIPETQRSVWSFDFEEKIQMNVTGTI
                     GDRVKLGVSYNTDASFEFENKTKIEYEGDEDDIVRKIEAGNVTLPLPGTLITGSQSLF
                     GIKTELQFGKLNVTTVLSQQKGQTQVVEVQGGAQVTPFEITSTEYEANKHFFLGHSFR
                     GNFDKAHKSLPQITSPITITKIEVWVTNKTRGKVEDVRPVYAFTDLGETNPSVLSNAA
                     SLQIAPNNTGWPSNGANTLYNIVKTPVSNRDDESLRTLGLVDGRDYDKTQSARKLTSS
                     EYSVDTKLGYISLNMSLNNDEILGVAYEYMVNGYTYKVGDLSTEVTSSKTPLVLKLLK
                     GTTLSPKLSTWDLMMKNIYNLNAYEISKDDFYLNVLYHDNKNGSDVSYIPEPAGRIDS
                     IALLKFLGLDNTNSQMNGNSDGVFDFIDGVTIDKRYGRVIFPMLEPFGRGLRDRFIKR
                     GVTDSLTWSKYTYYELYDSTKTKAEDVSEKNKFKLKGTYKSASSSEIYLNAMNIPKGS
                     VKVTSGGMPLVENQDYVVDYALGTVKITNSAIMSSGQPIKVSLENNANFNFQTKTMIG
                     THLNYKVNDNFDIGGTLLHLSETPLTSKVNIGDEPISNTIWGLNASYRANSQFLTTMV
                     DKLPLLQTKEMSSITVNAEFAQLIPGTSSKIGKKGVAYIDDFEASETKYDLKYITGWT
                     LSSTPQTRVEGNYFNNLDYNRDRARLSFYTIDESFYRKSSLTPAGLTDSMLNANDSRE
                     ILETELFPKKQNVNGVPTNMYILNLTFNPNKRGPYNFNTTDLDQNGNFITNPEARWGG
                     MMRALQTNDFEAANIGYIQFWVMDPFYNDSTNNNQGGDMYFQLGDISEDILKDGKMSF
                     EQGLPTPGDDSSKVVETVWGKVPDSDPITKAFVTGNNIRKYQDVGLDGLSNEDEAKIS
                     PAFAAKFQGTPIMDDPSGDDFKYFRNADFNNKDIVSRYEFYNGMENNSPEAGKDFSQS
                     NYSTPDMEDINDDNTLSDKENFFEYRVSMRPNDLVKGTNYVVDATTYSKGNIKARWVQ
                     FKIPIEDFTRKFGTIEDFKSIRFLRIYLTGFNQKTTFRFATLDLVRSEWRKYNGSLLQ
                     NSEGFGGQAPVSNFDLQSINIEENGDVYDIPSEDARPVDPTNSQYTLQNEQSMVLKVE
                     NLADDDARAVFKNGHVDMRQYKRLKMYTHAQPLNGEALQNGDVTVFVRFGTDNKENYY
                     EYEVPMIVSPIGTNISRDAMWPAKNQFDIELAKFLRLKQERNDADVSLLKRYVKPDGD
                     NNMYICGNPSLSDIRTIMIGVRNPSEANNKYSNDGAPKSAEIWLDELRLTDFSDQGGW
                     AANARAQIKLADFGNIRIAGATMQPGFGSIEKKVNDRAKETTNQIDASTDLELGKFFP
                     EKAQVRIPFYASYSQIDILPQYNPYDPDIPLQAAIDNAKTQAEKDKLRKEAIDHTLRR
                     SFSFTNVKVNKASKTPRFYDPANFSVSYGYNETKSYNYATKYKNYYHHEGGFNYIFNN
                     RPKNIMPLKKVSFLNKKAFQLIRDINFYYAPTSISFRTNMTHDFHEVQLKNLNDRSYE
                     LPKTIDQSFLWNRMYDIRYDLSRAIKVDFSATNAARIEEWDETMDKQYKNDPRYDVWK
                     QIKEGGRNINYKHQINITYTVPVNKLPLLDWTSLSTRYSGTYEWQRQPFMKSEEGAIV
                     EVGHNIANSRSIQINPGLNLNTLYNKVAFLRDLNAGNRRGKGAQKEKEKEMKTVSFEK
                     EGVSLKAGEAKSISHKLGSEDVTIKVTDSKGNEVKGTMEVASPNRLIFTPEKDVTGAK
                     IVVEGKVEAGPDVLMFLARNVVRLATGVKTISVSYTQTEGTALPGYIRPSKYLGMSGD
                     YAPGWQFVMGEQAPSLVERMVHKEWISNNELLSGSLLKNRTQSVNVRVNYEPFRGLRV
                     DLTGNHSFTQNISAAYLYTGNLSDTIQLYLSQFSRPMKSGNMSMTIISLGSWENMTKD
                     NYANSKYFDKFKEAAITAAQKLNENNQEIIARHGNSLDTGTQPGFNRKIQQALIPAFL
                     STYGGYGLDSKTLALFPVLSMIRPNWRVNYDGLIDIPIVGEYAQSVNLTHAYVATYNV
                     GSYTTNSKYDTRVDYSYVRDRNTGFYVPQFDVGSVSITEQFSPLIGVDVTLKNNITAR
                     AEYKKNRSLMLGTSNYSLIESYGKEYVFGAGYRFPDIPLTMLTLGAIASPIKSDLDLR
                     ADVSIRQDMNVMRKLDDGVAPSLTQGNTNVKISFTADYQLSDRLTVRLFYDRALTNPY
                     ISSSFMTIATDFGFSVRFVIAQ"
     gene            complement(22841..23422)
                     /gene="ruvA"
                     /locus_tag="PK252_04765"
     CDS             complement(22841..23422)
                     /gene="ruvA"
                     /locus_tag="PK252_04765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT14055.1"
                     /translation="MYDYITGKLTELTPTYLIVENNGIGYVMHISLNTYSKLEVNTQA
                     RIFAHLQIKEDAHTLYGFYEAKEREVFRILISVSGIGANTARMILSSLTAEEVANAIS
                     KENVRALQNIKGIGAKTAQRMIVELKDKMIKIAGSEQNLLPQYNTSADEALSALVMLG
                     FQKGAAEKALAQISRENKQASVEEYIKLALKVL"
     gene            23631..23936
                     /gene="rplU"
                     /locus_tag="PK252_04770"
                     /pseudo
     CDS             23631..23936
                     /gene="rplU"
                     /locus_tag="PK252_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            24045..24302
                     /gene="rpmA"
                     /locus_tag="PK252_04775"
     CDS             24045..24302
                     /gene="rpmA"
                     /locus_tag="PK252_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT14056.1"
                     /translation="MAHKKGVGSSKNGRDSLSKRLGVKIFGGEVVKAGNIIVRQRGTV
                     HNPGNNVGIGKDHTLFALVDGTVSFKKTRDNKSFVSVIAAE"
     gene            24396..25670
                     /gene="serS"
                     /locus_tag="PK252_04780"
     CDS             24396..25670
                     /gene="serS"
                     /locus_tag="PK252_04780"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT14057.1"
                     /translation="MLTLQVIREKRDFVLERLAVKNFKQPELVDKIIELDNQRRAIQQ
                     QADASQAEMNNLSKEIGILFKNGKKEEADAAKLKTNELKEKIGQLTEQLTVVEKELQN
                     TLVLLPNLPHTNVPKGHSAEDNLEIRKGGNVPALADDAMPHWDLAKKYDIIDFELGVK
                     ITGAGFPVYKDKGARLQRALINFFLDEARKAGYIEMMPPLMVNEDSGYGTGQLPDKEG
                     QMYHATLDNYYLIPTAEVPVTNIYRDVILNDSDFPIKNTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLEQMVEYVESLVNKLELPYRIIRLCGGDMSFASA
                     MTYDFEVFAAAQKRWLEVSSVSNFESFQANRMKLRFKEAGEKKTTLAHTLNGSALALP
                     RIVAALLENNQTAEGIKLPKALVPYCGFDMIN"
     gene            complement(25753..26991)
                     /locus_tag="PK252_04785"
     CDS             complement(25753..26991)
                     /locus_tag="PK252_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT14058.1"
                     /translation="MNYKVEFKGENVEGIIKVPASKSIANRLLIIQALCKEKFNINNL
                     SDSEDTVVLQRGLVSESDIIDIGHAGTSMRFLTAYFASQPGNKILTGSARMKERPIGS
                     LVEALQNLGADIEYVEKKGYPPLQIRGKQLNGKRIAIDSSISSQFISALLMVAPTFKG
                     GLELELEKNVISSSYIEMTISLMKLAGVLVKKQDNLLIVPEATYKMKDFTVEGDWSGV
                     SYWYQIAALAKKPDIAITSLYKESFQGDARCAEVFKHVGIETAYNKDGIALSKSTDIP
                     AHFEFDFIENPDIVQTLAVTCVMLGISFKFTGTQTLRIKETDRIAALQNELGKFGAVL
                     EYSNNGTLSWDGKRNSLAAIKEPIRIPTYHDHRMAMAFAPIALMGIPVIIENVDVVRK
                     SYPAFWTDLKAVGFSLTECE"
     gene            27127..27609
                     /locus_tag="PK252_04790"
     CDS             27127..27609
                     /locus_tag="PK252_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14059.1"
                     /translation="MRIENSAFVVRERKLMYLSTISLTILIPIIFLADFFNTPVLGLN
                     RELYIIGIALLYILLHLYRYWLNLNFIEAATEDNKLVFRYYSLRFFSAKHKSIEIPLA
                     DFIGFEINQPFKGLKKSLILFQRMKNKKAKYPPISISALTKEQIAELETFLKGLSKSI
                     "
     gene            27615..28724
                     /gene="dprA"
                     /locus_tag="PK252_04795"
     CDS             27615..28724
                     /gene="dprA"
                     /locus_tag="PK252_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT14060.1"
                     /translation="MDNSLLHAIALTMIPGIGCMTAKKLIAYTGSVEGVLKEKKSNLL
                     KIPGIGEYVADEILKSEVMTKAEAELAFIDKYNIKAWFYLDDNYPARLKHCPDSPVVL
                     YSKGNCNLDSPKMISIVGTRKATDYGKAFCEKLIFTLAERKHDVVIISGLAFGIDITS
                     HKAAIKAGLPTIAVIGHGMKTVYPAEHDKYAREIAKNGALVTEYTSDTKADRALFVRR
                     NRIIAGLSDAVIVVETGKKGGALATAEFANSYNRDVFALPGRIDDNHSQGCNMLIKQN
                     KAALIESAEDIEYLLGWASENENKPKVIQRNLFVELTEEEKVVMDVLNRHDSAHIDII
                     GVESEMPVSKVSSMLLKLEFDGMVRSLPGKIYRKV"
     gene            complement(28737..29951)
                     /locus_tag="PK252_04800"
     CDS             complement(28737..29951)
                     /locus_tag="PK252_04800"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOT14061.1"
                     /translation="MRKTSSLSFGIVAVLSIFLATPLSYSCSKDDKTPAAKGSIFTDT
                     SKLSKMNRYYVFIGTYTDGTGVAKSTGIQIYQMNPDSGSLKFVGVSSNTDNPSYLTIH
                     PSKKWLLAVNESWPGKITSFKIDTTNISLVEINSVASSGNSPCYISIDKTGKYAMAAN
                     YSSGSFASFGIGVDGTLTEVLSVLHGKGKSTNAIRQEGPHAHMIDQDTSIGFVYATDL
                     GIDKVHTISLDTTTGKLKLLTSLKSEAGAGPRHFAVHPAKHMLYVLHELKGIIEVSTI
                     KTADGSLQKIQSISSAPAVSAVYPGSADIHITADGRFLYVSNRGELNNIAIFAVNQTD
                     GTLQLIKHVACGGTAPRNFAIDPTGKFLLVANMDSNNIVVFKIDPTTGDLISTGIDIT
                     VPKPVCVKFLVL"
     gene            complement(30107..32443)
                     /locus_tag="PK252_04805"
     CDS             complement(30107..32443)
                     /locus_tag="PK252_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT14062.1"
                     /translation="MSDFLNQLNESQRAAVECTNGASLVIAGAGSGKTRVLTYKIAHL
                     LEKGIDARSILALTFTNKAAREMKERITKLVGEEASRNLQMGTFHSIFSKILRIESAS
                     LGYSTNYSIYDSSDSKSVIQAVIKSLSLDTQNYKPAIVQSCISMAKNNLITPAVYAAS
                     AEIIERDKAAKLSYIAEIYRLYNARCKASDAMDFDDLLLNTNILFRDFPDVLAKYQRY
                     IRYILVDEYQDTNFAQYLIIKKLAANHRNICVVGDDAQSIYSFRGAKIENILNFKNDY
                     PEYQLFKLEQNYRSTQNIVNAANSVIAKNSQQIPKNVFSKNAEGELVNVISNATDIEE
                     AYSIANEINTLKREDKVNLDEIAILYRTNAQSRVFEDAFRKMGIAYKIYGGLSFYQRK
                     EIKDLTSYFRLVINPNDNEAFKRCINEPARGIGDTTLEKLETSANENQTSLWNVINSE
                     MLGSINFNKGTLAKLEGFVKLIKGFAEMVGNTDAYNLARHIAVASGYLNALKEENTQE
                     SLNRIENIDELLNSIQKFIDDNAEENQIVTLNQFMETISLLTDADNEDETSREKVTLM
                     TIHASKGLEFNYVFVVGIEEDLFPSKRMVGSPAELEEERRLFYVAITRAAVKATLSYS
                     NFRYKHGSPTFCVPSRFIRDVDEKFLDTSRARDLYDTPKATTDGMPVDPRTKAYSRDL
                     RSVAEPKSTSTAVNEVRRTLTRIQEVNRKPVAIPENFEPDDPNSIKEGMTVLHQKFGP
                     GMVVSIEGSAPNNKATVVFEMDGEKTLLLKFARLKILN"
     gene            32669..34141
                     /locus_tag="PK252_04810"
     CDS             32669..34141
                     /locus_tag="PK252_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT14063.1"
                     /translation="MQTKLKQVSYKILLVISIVFSAYTEGYAQLYHVGANKDTLINYR
                     KTADFKAFYAKVFAYNVDIKAKEHIGKIKAISPDRQSGWTFKWWTYNFAERILKDTLS
                     VYSNIDSMELENLPTGCYKVQMSKGNVDTSFYAWIVINRSRFHLVKDDKGYIKYFEAT
                     CFKTDIRAIPSIGEKFPDSLRYVHSDNYVLPNPEDYQYNVFQNTPRIIIKGTDGSIYT
                     ADKNKYSITNPPSKDTRYFVEFSDKLNTLIRDTVVYKSIIPKAEFSLFISEDTMSEVS
                     TRFYTEKASGEAPLWAKVVSKSQNTNHLTWVLADSIKDPADSVYYFHTANDSINTISD
                     IPYKYPYKYKLILYAQNDRCTDSISKTLEVVNSKVGEQGANAFPKVFTPNGDGKNDYF
                     SIKSDTIRSIQFLHLFIVDSHGKKVYEYKGKVAQGWKGWDGKTSWGSDAKPGIYYYYF
                     EATGWGPIPKTGVTDPKQIKNSPNKVSGKGYFYLFRDSNN"
     gene            complement(34138..35202)
                     /locus_tag="PK252_04815"
     CDS             complement(34138..35202)
                     /locus_tag="PK252_04815"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOT14064.1"
                     /translation="MKLKIVFAFGILSLGLLAKAQYVEKAVLVDYNNISTEEAFIYYF
                     LPKTVIGATVFYSQINEKSGPYSQFAEEMLGITDIVKTNRTYWRIDSVKVSNFTVPDK
                     KQLVGIKPGKKFEMRFLQPIADAGFILSPQAIGFKQATTDNYIGEKQNIISEILRKPI
                     IRPTTDTTFRTVIRDSVTHRLPIIKNVTAAVSLRDRAKEAAQDIFKLRQRQYEIILGE
                     DEPHTNKKMFNAQLNALAEMERSYLMLFTGQTTTSSCQATFYYTPSDDLTTDASATLF
                     WFDEINGFSGKKGKAIKLSMIIEKENNATQVLPQGKNVVYYKMPVNVDFSIADNDKTY
                     FKGQIPVYQFGKIMPFIIVP"
     gene            complement(35264..36772)
                     /locus_tag="PK252_04820"
     CDS             complement(35264..36772)
                     /locus_tag="PK252_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT14065.1"
                     /translation="MLKILTSAQTREIDRMTIESEPIKSADLMERAAWQCTHWLMVRF
                     GKHQHFHIFCGSGNNGGDGLAIARQLDNQDYLATVYLVAGKDKLSPDAALNLGRLKNT
                     RVYVRFENIEEHLPAIKEDDVIVDAVLGSGLNKPLDGRLAELIEKLNKIKAYKIAIDI
                     PTGLMDVKANTGQTVFNAQHTLTFQFPFLSFFFAENAQFVGDWTVLDIGLDKNAVAQT
                     QVQYVLTETSDIHIKKRLKFGHKGTFGHALIIAGSRSMPGAAILSAKAALRCGCGLLT
                     SHVPSALAGILPVAINEAILSLDKGDNYTSNIPEISYYSAVAVGPGLGITNSTKTAFS
                     RLIKSRPKNLVIDADALNILASKPQLKNELPANTILTPHPKEFDRLFGSSANSEERLA
                     RLIENAISLDIIIVLKGHYSIVALPSGHCYFNPTGNNGMATAGSGDVLTGVIVSLLAQ
                     GYDAAHAAITGVFLHGLAGDIAAKELCQESLMANDIINALPKAFKQIETAKH"
     gene            36920..38572
                     /locus_tag="PK252_04825"
     CDS             36920..38572
                     /locus_tag="PK252_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOT14066.1"
                     /translation="MFKGHPKGLYVLFISNMGERFGFYTMFAILTLFLQDHFGWNESQ
                     ASILYGWFIMLSYLTPLLGGILADKFFGYGKAVILGLIVMALGYLVLSQGNGSTPYTI
                     YAGLFIVALGIGFFKGNLNVLVGNLYNTSELKSLRDSAFIIYYMGINAGALFAPYAAA
                     WAKSLFLQHAGWVYDPALPSVLHNIIAGIAPTTEQLALVNKYTNSADLAGFAHKYLEL
                     LSKGYLMAFGLAAIASVVSLGVFLLFKKHYKYADYTYKNASADAKEKELTPTETRQRL
                     VALGLVFLVVIFFWLAFHQNGSTLTFFARTYTNLEVGKFSGLFFSLPFIVCFVIFISL
                     LPSVFNKKIVAIRRSLYGAVSLVLLMVLAYFIYTQPGQSTIDPSLFQAFNPIFIILLG
                     PLVIWAFSLLNKRGAEPSTPAKIGLGMFITAIAFGLLIIPSIGMPVAESLQGATVSKS
                     IAASPFWLISNYFVITIAELCLSPMGLSFVSKISPPKYKGSMQAGWYVAMSIGNLLAG
                     LLGVYYYRWPLWVFFAMLAILSIVSGLLMMSLLRIIKQTEGK"
     gene            38789..40837
                     /locus_tag="PK252_04830"
     CDS             38789..40837
                     /locus_tag="PK252_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14067.1"
                     /translation="MARTYNIDSLKIEYKNATEDTSKIKVLSLIAVSYLDKQDYEKVI
                     ENCQAIIEIANGKNDRIEIIQAYYWLGISYYRKGNVNEALSYYLKALAIAKVIKNNYK
                     SDILNDIAVLYKGRGEYLKAIEYFQQNLEYAEQVKDSLMIGKTLNNMANVYFSYGIYQ
                     KSIEYYEKSIPIFHNIGKEMYVAYLYINLGLSYKKVNQRTKALESFNKSKEIFSSLKF
                     KAGIAQVNGELGTYYAEQGNYNMALRFHEQSLVVYIALGEKMLIAQTYQAIGEVYQKS
                     GAASTALTYYEKSLALFKELNAKKEMLDNYQNIASIYSVLGDYKKAYENFKTYSNLKD
                     STLSDDFLKQMQEMEAKYESAKKENEIKLQKAEISKKDAESKRKNIFMIAIGLIALMV
                     IGFAVLIFKQYSEKKKANILLAQQNDEIKKQRDQIFQQKKEITDSIHYASRIQRAVLP
                     ASKLLDDAGIKHFILYKPRDIVSGDFYWINQKDNKLIVAAADCTGHGVPGAFMSMLGM
                     AFLNEIVTKAEFNNAADILNQLRVLIVKSLHQSGKQEETKDGMDITLCMIDKENWNLQ
                     FAGAFNPLYLIRNGEIIEGPADRMPVGFHDKLEVPFTNTELPIQAGDTLYVFSDGYVD
                     QFGGENGKKFMAKRFKQLLLDIQDKPMDTQRDILDTNIIDWRGELDQVDDILVIGMRL
                     "
     gene            41108..41590
                     /locus_tag="PK252_04835"
     CDS             41108..41590
                     /locus_tag="PK252_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT14068.1"
                     /translation="MSVKTKRLIAIRKILSTSQIGTQEELLEELSNKGFQVTQATLSR
                     DLKMLKVGKMAEAGKGYVYFLPSDHENKAAKATDSRDSFPINGFLSLTFSKGLAVIKT
                     KSGYASSVASLIDSSDCYEIIGTIAGDDTILIIPVEDATPQDIRNALKLIMPDLENKI
                     "
     gene            41657..42469
                     /gene="proC"
                     /locus_tag="PK252_04840"
     CDS             41657..42469
                     /gene="proC"
                     /locus_tag="PK252_04840"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT14069.1"
                     /translation="MGSVKNTKITILGGGNIGMSIAAGLIKSGQWKASDLTITRVASQ
                     SLTDIVNMGINATHDNKKALKDADFVFLAVQPRQAVKVLEDIKEALDPKKHNIISVVT
                     ALSFADIEKVIGKGFSLTRIMPNTAISICESMTCMASNDTESEMFKKAQALVNHLGKT
                     LIIEEKMMAAATVLASCGTAFMMRFIRAAAQGGIQVGFHASEAQYIVAQVAKGAACLL
                     MENGLHPEQEIDKVTTPMGLTIKGLNEMEHNGLSSAVIKGIVHSYNEMEKIK"
     gene            42485..43813
                     /gene="argH"
                     /locus_tag="PK252_04845"
     CDS             42485..43813
                     /gene="argH"
                     /locus_tag="PK252_04845"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT14070.1"
                     /translation="MKLWDKGTPVNQRVEQFTVGRDREMDKMLAKADILGSLAHIAML
                     NSIGLIADDELKLLSAALKDIYKNHLTDFTIEPGVEDVHSQIELMLTRKLGDVGKKIH
                     SGRSRNDQVLVDIKIFAREQIREIHDLILTLFETLVALSEKHKNVLIPGYTHLQIAMP
                     SSFGLWFGAYAESLTDDLLMLQTAYRITNQNPLGSAAGYGSSFPLNRTMTTKLLGFET
                     LNFNVIYAQMNRGKTEKTISFALSSIAATLAKMSMDICLFMSQNFSFVSFPDELTTGS
                     SIMPHKKNPDVFELIRAKGNRIQALPNEISMMLTNLTSGYHRDFQLLKEILFPAMEDM
                     KAMLDIANMMLQNIRVKDDILKDEKYKYIFSVEEVNRLVLKGVPFRDAYKQVGGQIAE
                     GKFNPSLTVKHTHEGSIGNLCNENIRKKMQNISEGFCFANVDKALQHLLQ"
     gene            43840..44688
                     /gene="proB"
                     /locus_tag="PK252_04850"
     CDS             43840..44688
                     /gene="proB"
                     /locus_tag="PK252_04850"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT14071.1"
                     /translation="MKDRARLKEKEKIVIKIGTSSLTHDNGKINFKSFEKLASVISQM
                     VNDGKKVLLVTSGAIAVGAGKIGLGQKPSKLSEKQALAAIGQAELIKIYEKFFADHNK
                     ISAQVLLTKDGVLNPIRRQNAKNTLNTLLSMGIVPIINENDTVSTDEIEFGDNDTLSA
                     HVAVVSEADLLVILSDIDGLFDGDPRKDANAKIISKVGNVDDVEKYAGASNSEFAKGG
                     MITKIAAARLCNNAGIDMVIAKGEDPAIVLDIIAGKDLGTLFVGKTEISIKKPEESVI
                     YKEKRG"
     gene            44768..45553
                     /locus_tag="PK252_04855"
     CDS             44768..45553
                     /locus_tag="PK252_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14072.1"
                     /translation="MTKSKIDKITIHSKCLGKEMNVLVYLPYDYDKKENLTTLYFLHG
                     RSGDENILIEAGINIVADRLIANQEIESMIIVCPNIENSRGLNSSFNCKEVRDPLGRI
                     INIGMYEDYLIREVIPEIDNRYNTNRNRNGRYIGGASAGGYAALHNAFRHYDLFSKVG
                     GHMPAIELKLEDEDKAYFQNQDIWEKYDPITIAQQMKSCDLKVYLDAGDKDEGRFYEG
                     CLILNKILNEKGIDSQNHVFEGCHSLEYIKSNFEKYMFFYGKK"
     gene            45716..47017
                     /locus_tag="PK252_04860"
     CDS             45716..47017
                     /locus_tag="PK252_04860"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT14073.1"
                     /translation="MSQIIDIAKQAKKASIPMSALSAKTKNNALKAIANALVKAEAQI
                     IEANTKDLQKAEQEKLAAPLLKRLKFDKNKIKEVVDGIESLIRLAEPVGQTLSATELD
                     NGLELYKVSCPIGVIGVIFESRPDALVQISSLCLKSGNAVLLKGGSEALHTNRILTEI
                     ITKASADAGIPQGWLHLLETRSDVNEMLKMDDYIDLIIPRGSNEFVKYIMDNSNIAVL
                     GHADGICHVYVDKEADIDMAVSVAADSKTQYVAVCNAAETLLVHSSIAPKFLPALKEK
                     MDALHVELRGCEKTLKYISVKPASEQDWATEYLDYIWSVKVVDSMDEAIEHINKYGSG
                     HTESIITKDNVAALKFMDLCDSGNVFWNASTRFSDGFRYGLGAEVGISTNKIHARGPV
                     GLEGLLIYKWKLIGNGQIVADYASGKKVFTHKKLDKKFNPS"
     gene            47128..47805
                     /gene="ung"
                     /locus_tag="PK252_04865"
     CDS             47128..47805
                     /gene="ung"
                     /locus_tag="PK252_04865"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT14074.1"
                     /translation="MADNIQIEDSWKELLADEFESGYFAALKTFLIEEKQKYEVFPPG
                     SLIFSAFNLTPVDKVKVVIVGQDPYHGKGQAHGLCFSVQRGIKPPPSLVNIFKELQDD
                     IGIAIPSHGCLDSWAKQGVLLLNATLTVRANTPRSHFGKGWEKFTDAAIKLLSDKRNN
                     LVFFLWGNDAKAKETLIDSKKHLILKAAHPSPFSAYNGFLGCKHFSKANQYLVQHGYE
                     PVDWNLK"
     gene            complement(47825..48754)
                     /locus_tag="PK252_04870"
     CDS             complement(47825..48754)
                     /locus_tag="PK252_04870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT14075.1"
                     /translation="MRYNWFENATPGLKIMLSIAFMATFLLIGMFLSVLFAIPVWGND
                     ILKTLTAGADIHSTTGINILKYLQTFQSIFLFVLPPLALPFFFGQRPLNYLYLKERPT
                     AKSALLAILILICAVPFINFTEHINSLMKLPSWLSGIESWMKDAEKNAQSLTEAFLKT
                     STISGLLLNLFIMALIPAVGEEFVFRGLFQRIFTEWTKNAHWGIIIAAAVFSSIHFQF
                     YGFIPRFLLGVMFGYLLLWSGSIWLPVLSHFLNNALGVVFFYLNFNHYTSIDIDKAGV
                     ESALNLPVLFTSIFLTGILLFYFWKQKKQETEI"
     gene            48888..49895
                     /gene="dusB"
                     /locus_tag="PK252_04875"
     CDS             48888..49895
                     /gene="dusB"
                     /locus_tag="PK252_04875"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT14076.1"
                     /translation="MKIADIELGERPVFLAPMEEVTDVSFRRFCKEFGADMMYTEFIS
                     SDGLIRNAGKSLKKLEIFDYERPIGIQIYGHLKDAMVEAAIIAERANPDVIDINFGCP
                     MKKIVRRGAGSGMMNNVPLMVEITDAIVKAVKKPVTVKTRLGYDEENKNIVDIAERLQ
                     DVGIKAITIHGRTRSQLYTGKADWTLIGAVKNNPRMTIPVIGNGDIRSGFDAYEAFEK
                     YGVDGIMIGRATVGCPWIFEEIKHFLKHKSPLPPKNVAEVSTLVKKHLLLSVELKGER
                     GGILEMRRHFAHYYRGLPNFRETRIKLLTSLNVDEILQIIDSIDERYGELFMPPVDEK
                     H"
     gene            49984..50721
                     /locus_tag="PK252_04880"
     CDS             49984..50721
                     /locus_tag="PK252_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14077.1"
                     /translation="MRTFHSKIFIFCVFVLTGISLAYSQQANDGVSSKGPANYDGTKT
                     INAQTNDTAKVALKQETIDNAPKADVAPANTVISEQTTSERYDTIVKKTGGKMKVKIT
                     GKNVHEVTFFMPGEKKEKRLSTGYIKELRYADGRIDLIDNTPEKITAKDPILLIPESE
                     WKKVEVAKSEIEVKDLVEKEVIEAEAEGKKYTTTDSFLEKNAIVILRKKAVNLNATKI
                     LVIDKIVTREYGEFPKMKVIAKAYANE"
     gene            50774..51250
                     /gene="coaD"
                     /locus_tag="PK252_04885"
     CDS             50774..51250
                     /gene="coaD"
                     /locus_tag="PK252_04885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT14078.1"
                     /translation="MERIALFPGSFDPFTIGHESIVKRGLSLFDKIIIAIGSNSDKAG
                     FFPVEKRIQWIKQVFEYEEKVMVAQYTGLTVEFCKEVGAQFIIRGLRTSADFEYERSI
                     AQVNKAMAPEIESVFILTAPEHASVTSSIVRDIIRHGGDASKFLPNSQELKNLRIH"
     gene            51270..51617
                     /locus_tag="PK252_04890"
     CDS             51270..51617
                     /locus_tag="PK252_04890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT14079.1"
                     /translation="MIYKKDQAAEFEKQGVKMRVYNSKEQCPEASVVYQETQTGHHEE
                     FYHSKSNFIFYIIEGAGTWFIEDIPYDVEAGDVVIVPPGKRFYYKGKLKQVCITSPSW
                     EPQYEHHVRDVEF"
     gene            complement(51628..52086)
                     /gene="smpB"
                     /locus_tag="PK252_04895"
     CDS             complement(51628..52086)
                     /gene="smpB"
                     /locus_tag="PK252_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT14080.1"
                     /translation="MKTEQQKIDIRNKKASFEYEFLERYTAGIKLVGTEIKSIRAGKA
                     NLSDAYCYFVNGELYVKNMHVAEYDWGTCNNHEPRRERKLLLNKKELDKLYKKSQDKG
                     LTIVCISLWINERGLAKLDIALARGKKQFDKREDLKRKDASREMDRAMKQ"
     gene            complement(52140..52748)
                     /locus_tag="PK252_04900"
     CDS             complement(52140..52748)
                     /locus_tag="PK252_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14081.1"
                     /translation="MQKYKQLWEKTKKIILHPDEGWAEIFVEPQEMRWHMQNYVLPAM
                     GVLAVAILAGYTFSAITVGNYSFVYCLFKAIEVVAASFFSLYVSSLLVYEISIRKIAQ
                     IDYDKLFILMLYSLAFFWVATALAGILANYPTLGSFFMFLGIYGMYPFYRGAKMAAIA
                     DPLTIMKILLLAFACIVITYLAIHWSFSFILKPLHYSHALQQ"
     gene            52817..>53340
                     /locus_tag="PK252_04905"
     CDS             52817..>53340
                     /locus_tag="PK252_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOT14082.1"
                     /translation="MLSSKYNLLTILGPTACGKTQLAVAAAAKLGSCVISADSRQVYR
                     QMDIGTGKDLSEYSFEGSKVPHYLIDICQPGEKYNVYEFQKDFFNVYEKVYAENAIPV
                     LCGGSGLYIEAVLSNYQLLSVPVNEDLRNSLKNKSLEELATILASYKLMHNKSDVDTV
                     KRAIRAIEIAEYQK"
BASE COUNT        16597 a        10470 c        10444 g        15829 t
ORIGIN      
        1 aaaatgcttc gcctaaaggc gagggatttc acccatccca gaattataaa ttaaacattt
       61 tctaatgctt aatttgggct taaccaatct aatttgtagt tcaactaata aactgttgtc
      121 gttttcttct catgatcata aaataacctc caatgctgaa atcattggag gtaaaacgaa
      181 tggtaaaaac ttatgaagac taagaaatgt ttattaaatc ttaaaatatg ccactatctc
      241 tttcagttct gtggccaggg tttctaactt attagtatga ctggctataa actcggccga
      301 agaggcattt tcttgtattt tcttgttcaa aaccagtact gcgttagtga tttggttgat
      361 gttggtggtt tgttcggcac tgctataggt tatttcttct atcagttgag ccgttttttc
      421 aatctcaggc ataatttcct gcagcttatt gcttgcgttg gttgtttgat ttagtccggc
      481 tttggtgagg tcgttaattt cgttggcagt tattttactt ctctcggcaa gtttgcgtac
      541 ttcgttagcc actaccgaaa agccttttcc gtgttcttgc gcccttgcag cttctacggc
      601 agcattgagc gataatatgt tagtttgaaa ggccaaatca cttatcatcg atgtatagtt
      661 gtctatctta gatattgatt cggctgatat tttcgaaagg gtattaactg ctttcacttc
      721 actggtggca tgcaaggcga tgtctttcac tttttcaagg tgtagcatgt tgctttctac
      781 atttgttgat atttcttcaa tactggccga aatttcttct gctgatgcgg cttggttttg
      841 tgcgttgtca gacatggtac acaccttgtc gttaagggta gcgcttgcct gggtgagttg
      901 ttctgaacca tcgcgaataa ggccaatcac atgtattagc gtttgctgca tgttttgaag
      961 ggcaattgat aattcgccaa tttcatcatg gcttttaatt tttatacttg tattgagctc
     1021 tccattttca atatgtttgg caaatgtaac tgcttctttc aatggattga caactgattt
     1081 gactatccaa aacgatacaa atactgatat tattattact atcaatcccg aaataatggt
     1141 ataaacctga aatgctgagt aaacatcgtt agtctctttc tgcattgcct tcaacttggc
     1201 gtttattatt gtttgcacca tggcaatatc atcaagagct tttctacttt gcactgaaag
     1261 cagaccattt tggtcaacca aaggagatac agacatcata gcaatagggt cttcgtagtc
     1321 tgccatttta gaaagccttg tcattatcat gttttgagtc ctgaacagcg tatcttttat
     1381 ttcttgcgat attttttcat agttttcttt tacataattt gcttttgggc tgtcccattt
     1441 atataaaagc gctttgattg tgctgtctgt actgaaaaat gtctgctggt gaatctttcg
     1501 gagtttccct ttgttgggcg tattgtcaaa tttttcaata taaacccagc tttttgtcaa
     1561 tatttctgag ccttctacaa ggctgtaaag ctcattgagg gcagctgaaa ttggctcaag
     1621 cactcttgta gtttcgcgtt tcaatatctc attgcgatac gaaatgaaaa cggtaaaagt
     1681 aaggttgata agtattaaaa gcaggattat gctaaacccg agctttattt tttttgatac
     1741 ggtcaaattc attaatgatg atggttttgt ttgaaaaatg gccaattact tttttataca
     1801 tcgaaccgaa aaggcatagt ttttattaaa aaaacttttg gtcaacatgt tacttttatt
     1861 ggtaaaaaag taataccacg cgtgttctgt atcagatgag gttgctgacc aaaaaccgcc
     1921 gaaggtccca acaaacatgt atgacgaacc attggcataa ccgccatata aagcattaaa
     1981 tccggaggcc ccatcagcct ttagctttcc ggcagctaca tcttcgcctc ccaactgtga
     2041 tataagttcg tcccaatcgc tttcggtagg taaacgccaa cctgccgggc atgctttcat
     2101 tgctgcctga taagtgtata acctgccata catagctgtg ctttgggcat ttgcataagg
     2161 aaaacttccg gctatttcta aatccagatt cttggccatc catacctggt tgccaatttt
     2221 aaccgattta acttgagcgc ttgtccctat tagaaaatta acagcacata aaacagcaat
     2281 ggaaatacgt aataagttca tcatggtata attttttatt agaaatgagt ttgttttttt
     2341 tctgaagctt taaaaataaa ttttgctttt ttcgtatttg tattttaaac cccatatagt
     2401 catacaagaa tctataagcc aactataata gttttgatat agtttaaaat atattgttat
     2461 attataattt cttaatcttt tagggataaa acaagtttgt tttatctgta aataattttt
     2521 ataatgttgg atgacccccg aaggacaact cgtcccgact attcgggatt catccttgtt
     2581 tagctccatt aagcttgcaa gcttggtggt gtttaaccat tcatggttgt ttcttttttt
     2641 tggttcaaaa atactttgtg ccaaaatttt gtccgttaaa tttgttttat ctctttttta
     2701 tgtaattgtt aaaacttttt atttaattgt atttacagcc atgtatcaaa tcagaatatg
     2761 acctgtttat ctcaaattta tattagggta actttataaa gcaaatcatt gaaatataga
     2821 attttaattg attttgtgtt gcttaatgtt taaaatgggt taagtgtata aaatgtctaa
     2881 tacaaaaaag ctacaagagt ttttattaag taactttgca ctgcaaaaat gaaaatatgg
     2941 ctttaatcaa atcggtaaga gggtttaccc caaaaattgg gaataatgtt tatttggctg
     3001 ataatgctac tataattgga gaagttgaaa ttggggacga ttgtagtatt tggttcaaca
     3061 ctgtcatcag aggagacgta aatggtatta aaattggcaa taaggtaaat attcaggatg
     3121 gaagtgtatt acattgcctt tatgaaaaat caaccataga aataggcaat aatgtatcta
     3181 ttggccacaa tgtagtaatt catggggcaa agttgtgcaa caatgtttta attggtatgg
     3241 gagctattgt tatggaccat gccgttgttg gcgagaactc aattattgca gccggctcag
     3301 tggtattgga tagtacaatt attgaaccag atagtattta tgccggagtt cctgcaaagt
     3361 ttgtcaaaaa ggttgaccca aagcaatctg ccgagatgat acaacgtata gccaataatt
     3421 acctgatgta cgccagctgg tacaaagaag gtaaagaatg aaacttttat attaccataa
     3481 aatgttgttc aaacatgtca aaatttagtt tcatccgtaa atagtttttt taataaagtc
     3541 ggacgaaccc caaaaagctc ttcgtaagaa agctcgtccc gactattcgg gactcatcct
     3601 tgtttatgtt ttaacgaacc atgctcgttt catgttaatt ttgtcgtttt atgcgcctca
     3661 atttcccgac ttcttttata cccctttacc ttcttgtttt ttaaaactca ataatcacgc
     3721 caacagtagg aagtattgtg cctccaccat cattttcaag agtttttaaa gtatatctgt
     3781 cgggatttgg tatgataggc acacttcggt caaccaaata agttggaggc gaatctgact
     3841 taaagttgta aacattctga atatccatat atgctgttaa cgaccaacgg ttgaagaaaa
     3901 actctttgtc cacacggatg tccatctgat gaaatgcttt caacctcaat gaattaaacc
     3961 tgctataatc gaggtaggcc tgattgttaa catcccatgc ttctacaagt gacgaacgtt
     4021 gtatatctac cggagtataa ggggctccac caacaagcct gaatttggaa cctacagtcc
     4081 agttgtttcg gaattcgtaa gccaccaaaa tattcagcag gtgcttattg tcccatgccg
     4141 aagggctgta tcctccgtag gctgttttaa attcgctacg caccaaagta tatgacaaca
     4201 taacattagc tcccatcaag tcgcggtttt gatataacaa ttctgccccg tatgccctgc
     4261 ctacccccgt cgaagtaacg ggttcatcgc catacgtgcc ataatcaacc gtttttccgg
     4321 caagggcaat accatcacgg atagaaaaag gatagttgct ataatttttg taaaaacctt
     4381 caatagagaa ttttgaattg ctggttggca atatatcaaa gcctgcaaca atgtggtcgg
     4441 cctgcatgta tttgaggttg ttgaatttat tgataagtac acccaaagtg tctctgtatc
     4501 caagtgttgt gtatgaaggc aattgataat atcttcctac gcttgaattt agtgttaatg
     4561 actgtgtcaa agcataagac acactaagcc ttggcgaaaa ctggtttaca aagttcttca
     4621 tttgactggt ataattattg gcatcagacc tcaagccaaa agaaatagta agttttttgt
     4681 caagcagctc tttgctggcc tgcccgaaaa agttccattt gaataagtcg aaatctgatt
     4741 tgtacaaatc ttcacctcca aaaagttttc ggtatgtgct gttatagtat cgggcatatt
     4801 caaaaccggc ccctgcaatg actttcaaac ccaaaaggtt ggctctgttg tattcatagc
     4861 gaaatttgtt ctcagtctca aacgaattat agtccaaaag cttattttca ggcaattcga
     4921 tgttatttgc atattttatc tggttattgt tgagcatact acggctaacc acccaggtat
     4981 cataactgtg ctcgtgaaaa tgcttataag tagcaccaat agtgtaattc cattgcgaat
     5041 aagcaggtaa atagctcaaa atatactttt gtttctcagt tttgttggcc tcaagattaa
     5101 ggtcgctaac atcataagcg ccaatactca taaatgtcag ctcgtttttg ttgtcaagac
     5161 gggtttttac ctttatttga taatcattgt aaataggtaa aaaagggagc tcaagtacgc
     5221 tgaatagaaa ctgtaaatag cttctgcgtg ctgagagtat gaaatttgta ttttcaccga
     5281 tagggccatc aagggtaagt gcaaagtcag aagccccaat gccgcttttt accttcacct
     5341 tttccgaatt gccctctatt tgcttgatgt ctaatataga acttagtgca ttgcctttat
     5401 ctgcaggaaa agcgccggaa taaaagctta cttccctgat aaggtcagca ttgatgatgc
     5461 ccacagcccc acctgacgaa ccttgggtag caaagtggtt aagattgggt atctcgatgc
     5521 catcaagata aaagcggttt tcactggcac cacctccacg cactactacg tcattacggt
     5581 aattgggagt agaagcaacc ccggggaaag actgtataac ccttgaaata tcgcgattgc
     5641 cgcccgggct tttttcaatc tctttcagtc caatgttacg aagcgacaca ggactctctt
     5701 tgtcgcgtct aaacggagaa gctttgacta caacctcttc aatttgttgg gtagcctcaa
     5761 ccatcttcac ttccacaaaa gttttgccag cgttagtaac acgcaactgt tctgaaatat
     5821 aaggttcaaa accaagggca gtcaccctga tttctataaa tcccggagga acattgttaa
     5881 aagtaaagtt tcccttgtcg tctgtgattg agccaaagtt ggtgccatag atagccacag
     5941 aagcaaactc tatgggcatg ttattcgaag catcataaac agttccctga accaccccat
     6001 ttgatttttg ggctatcagg atgtttgacc caaacaataa aactgaaatg tataatatat
     6061 ttttcatagc atctgttttt gtagataaac agatgcaaaa gtattttgtt taaaaaataa
     6121 acaaaataat tgaacgaaaa atctgccgtc gaaatactga ggtttttaag caaaaaccta
     6181 acctattaat gttacagcta aacagtacgc aactcaaagt ttttgccaag ataaacccga
     6241 cgtacctgct cgtcattggc caaatcttcg gcagtccccg attttagtat ctcgccttcg
     6301 tacaataaat aagccctgtc agtaatacta agggtttcgt gcacgttgtg gtcggtaatg
     6361 attaccccaa tgtttttttg cttcaatttt tttacaatct cctgaatatc ttcaactgca
     6421 atagggtcaa cacccgcaaa aggctcatca aggaggataa agctagggtt tatagccaaa
     6481 gcccttgcta tttcagttct gcggcgttcg cctccagaaa gctggatacc aaggctttta
     6541 cgtattttgt gcaggctaaa ttcagtcaaa agactttcaa ctctttcatt ttgttcatct
     6601 tttgagagct tggtcatttc gagtacagcc ctgaggttgt cttccacgct tagttgcctg
     6661 aatacagaag cttcctgcgc cagatagcca ataccctttt gggcacgtct gtacataggt
     6721 tcatgggtaa tctcctcatc atcaagaaaa atttttcctt cattgggcgt gataaggcca
     6781 acaatcatat aaaaagaagt tgtctttccg gcaccattag ggcccaaaag gcctacaatc
     6841 tcaccttttt caacctgaaa ggatgcgctt ttaacaacag tacggctttt atatcgttta
     6901 accaggtttt cagtacgtaa aatcatcaga aaaatttttg taaagataaa atttgatttt
     6961 gaacaaaaat aaaattgtta cgctaacaac caactactac ctctataata actaccaact
     7021 gttaactaac tgcctgccct ttcccgctta cgcaccaccc atgcagaaat agaaatactg
     7081 cccatataca aagcaaaaag aggaaatgca accaaaacct ggctcaccca atccggagga
     7141 gtaatgatgg ccgcaataat aagaattact actatggcat gtcgccagaa ttttttcatc
     7201 aaagccgggg taactaaacc aattgacgaa agaaaataca caacaacagg caattcgaat
     7261 accaatccgc aagccaatac cacagaagtt acattggaaa tatacgaaac aaaattgata
     7321 tggttttcta ccaggtcaga aaccgaataa gtagtaagaa agtcaataga aaaaggtaaa
     7381 aggataaaat agccaaacat aatgcctgtc ataaaaagca atgaactaac caccacagct
     7441 ccacgggcag cttttctttc cttttcgcgc aatgcaggag ctacaaagct ccaaaactga
     7501 taaaatacat acggaaaagc cagaataaaa ccaccaacaa tggcaaccca aatgtgcata
     7561 ttgaattgcc cgcccatggt aatgttttgc aaaggggctg gatggctgtt gatacaaagt
     7621 gatgttgtcc ctaaataacc gccaactctg caaagcaacc tgtttgaaat aaaaccagga
     7681 cttcggggcc ccaacaatat tacgtcaaaa acaatgtttt taaagaaaaa aaccaaggca
     7741 gctcctataa caataccaat caatgctttg ataataaccc atctcagttc ctcaaggtgc
     7801 tgcaaaaaag tcatttcccc atcaggtttc attacacaat caactttggg tttaaaaatt
     7861 tttttcgtaa aggtatgatt tttgtatata cattttttac aattaacatt ttgtcacatt
     7921 tatgcaaaag ttgatgataa tgttaatatt ttcaatacct tgaccaataa ccttgccagt
     7981 tgtttacaaa tgagcgtttt cacccatttt gatgatattc gtcatttaat ttttataaga
     8041 ttggatagtt tgaagtaaat tagcataaat ttcaaaaagt gataaaaacg cttaaccgtg
     8101 caatgttttc aatcacttac actacaaaaa ggttattaac actatacttt gtcagataaa
     8161 aagtcattgt tgatgataaa agaagactta tcactccagg attatttaaa aaaatacttt
     8221 gggtttacca gtttcaaagg gaaccaggaa gctatcatcc gcaacttgct tgacgggaat
     8281 gattcatttg tgctaatgcc cactggtggg ggaaaatcgt tatgctacca gttgccagcc
     8341 ctaatcatgg aaggtacggc cattgtcatc tctccgctca ttgcgctgat gaaaaaccag
     8401 gtagatgcca tgcgtacatt tagtgaagaa gatggcgtag ctcactttct caattcatca
     8461 ctcaacaaaa cacagataaa taaagttaaa gaagatgttc tgtcgcaaag gaccaagctt
     8521 ctctatgttg cacccgaatc actgacaaaa gaagaaaacg ttgctttcct caagcaaata
     8581 aaagtatcat tttacgctgt tgacgaagct cactgtattt cagaatgggg acacgacttt
     8641 aggccagaat acaggcgcat caggccaatt ataaaccaga taggccatgc cccaattata
     8701 gctttaaccg caacagcaac cccaaaagta caaaacgaca tacagaaaaa cttagaaatg
     8761 cttgatgcta aggttttcaa gtcttcattt aaccgggcca acctctatta tgaagtcagg
     8821 ccaaaaattg accctgcaaa acaaattata aaattcatca aaagtaaagg ttcaaagtct
     8881 ggtattattt actgcttgag ccgtaaaaaa gtagaagagc ttactgaact tttaaaagta
     8941 aatggaattc gtgcacttcc ttaccatgcc ggaatggatg cggctacccg tgctaccaac
     9001 caggacaaat ttctgatgga agaagctgaa gtcattgttg ctaccattgc attcggtatg
     9061 gggattgata agcccgatgt gaggtatgtt attcactacg atattcctaa aagccttgaa
     9121 ggatattatc aagaaacagg ccgggctggc cgggacggag gtgaaggcca ttgtttgacg
     9181 ttttacaaat accaggatat ccaaaagctt gagaaattca tgcaaggcaa gcctattgct
     9241 gaacaagaaa taggcaacca gctattgcag gaaaccgtat cgtatgccga atcatcggtt
     9301 tgtcgaagaa agtttttgct ttattatttt ggtgaggttt acgaaaagga caactgtgaa
     9361 aactgtgata actgcctgca cccaaaaaca cagtttgaag gacaggaata tattgagcag
     9421 gttttggaat gtattcttgc cgtaaaagag aagtttaaag gtgaacatat tgccaatatc
     9481 ctgtcaggca atctgactac agccataaaa tcttataagc ataataagct tgagctattt
     9541 ggtgttgggg cagatagaga cataaaattc tggaatggga ttatcaggca gatgctggta
     9601 aaccaattta ttgccaaaga aatagaaaac tacgggcttc taaaagtgac cgaaaaagga
     9661 ctgcaatatc ttgaaaagcc ttattctata atgtttaccg aggatcacga ctatgaagta
     9721 gaaaacgaag aagaccaggt ggtaatggtt ggaaaaacca ccactgtgga cgaagaatta
     9781 cttcatatat tgaaagacct gcgaaaaaaa atagcgaagc aaaaaaacct tccgcctttt
     9841 gttatttttc aagatccttc acttgaagac atggccattc aatacccaat ttcggtagaa
     9901 gaactgcaaa atatagccgg agttggggct ggaaaagcaa tgcgctatgg caaagaattt
     9961 gttgagctta tcaaatcgta tgtagaagag aaagatatca ttaggcccca ggatatggtg
    10021 gtcaaatctg tggtgaataa gtcggggctc aaagttcaca ttatccaaag tatcgaccgc
    10081 aagatgaacc ttgaggatat tgctgcttcc aaaaacctca gcttacaaga actcattact
    10141 gaaattgagg ccattgtaaa tagcggcacc aaactcaata tagactatta catcaacgag
    10201 atacttgatg aagaccatca gcaagaaata tatgattact tccgtgaagc cgaaaccgaa
    10261 tcaatagaag atgcccttag cgaattaggc acagatgaat atagcgaaga agaagtaaga
    10321 ctgatgcgaa tcaaatttat gtcagaaatg gcaaattaac tacggttacc ctccatttcc
    10381 atcctgtcaa gctctttttg actgtattcg caaagtaatt cgaaatatgc aaaaaaatct
    10441 tttttcaggc tttgatagtt tttgtgcatc acataaatag caaaagcatt ttccggaggc
    10501 attgatgtac ccttactcat cccgattaag acactctcaa ttccttcgag ggtgcgatat
    10561 gactgcaacc aactgttttt tataaaacca ggcattatcc gtcgcatctc cagcgggata
    10621 ctttccatat aatcatttaa ccaatcgtgg acctgagcta catagtcatc aaaaggtata
    10681 tccgaaaagt gatgccattc tgaagccaga aaatggtcat aaattatatc ggtaaccacc
    10741 ccggcatgct tacggtactt aggttgaaac aataacttat gccgttttac aatcggatgg
    10801 gtatctgtaa agtaatcaat ttttctgtgc aacaatatcc cgcgttgtac aagcgttgga
    10861 taacgtgtat gttctgcacc ctttacatag tcggcaataa aatttcccag ccttatttca
    10921 ttgctattcc ctgagagata tatgtgagcc agaaaattca ttttttcatc caaatcgtta
    10981 ttatacaaat ttaaaggttt attttctgtt tccagcgaaa aaatctaagt atttaaaagc
    11041 ttccaagcat gaaaattcat aaatttactg taatgaacct aaataaggcg ctatgagaaa
    11101 acgcattatc atcatcgggg ctgctggtcg cgattttcac aacttcaata tgttgtttcg
    11161 tgacaatgaa ttgacagaaa tagtagggtt tacagcagca caaataccag atattgatgg
    11221 aagaaaatac ccagcagagc ttgccggaaa attataccct gacgggatac ccatttttgc
    11281 cgagaaagaa cttcctatgc ttatcaaaac cctgaaaata gatgaatgcc tattttcata
    11341 cagcgatgta tcatattcca aaattatgtc tcttgcctct gttgttaatg cagccggagc
    11401 tcattttacc gtggccggaa ctacaaatac catgctgaaa agcaataaac cggtaattgc
    11461 cgtaggggct gtccgaacag ggtgcggcaa aagccaaact tcacgaaaaa tagctgaaat
    11521 aattaatcag aaaggaataa gaacaattgc tatccgacat ccgatgcctt acggtgattt
    11581 ggtaaagcaa aaagtacaaa gatttgcatc catagaggat ttgcaaaagc atgagtgtac
    11641 agtagaggaa atggaggaat atgagccaca tatcatgcgc ggaataccca tatatgcagg
    11701 cgttgattat gaagcaatac tgcgtcaggc agaaaatgac cccaaaggat gtgatgttat
    11761 tttgtgggat ggtggaaata atgacttccc gttcattaac ccagatctga tggtaaccgt
    11821 gctggatcca ttgcgaccgg gacatgaagt tgcttattat cctggagaaa caacattgcg
    11881 aattgccaac atagctatta tcaataaaat tgactcctca actcccgaaa acgtagaatt
    11941 ggtaaagaat aacattgcta aggtcaatcc aaatgctatt gttattgaag ccaactcacc
    12001 aataagcgtt gaaaatgcca atattattaa aaataaaagg gtattggtaa tagaagatgg
    12061 cccaacgcta acgcatggcg aaatgaaaat tggcgcaggc attgttgccg cagaaaaatt
    12121 tggagcttca gaaataatag accccaggcc atacctggtt gggaaactta aagacacttt
    12181 ccaaatttat cccaacatag ggaaactgct tccagccatg ggctatggac aacaacaact
    12241 ggcagacctc gaacaaacca ttaacaatac tgtctgtgat gcagtgattg ttggcactcc
    12301 cattaacctt ggccgaatta tcaatatcaa caagccatac acgcgtgttt catacgaatt
    12361 gcaggaaatc agtcatcctg atttagaaac tttattaaac gaatttttaa aagaaaaaca
    12421 tttttactaa acttacagaa aaataacccc aaaccagacc tatgttagta aaaacatttg
    12481 gtagtgccgt acacggaata aatgccacta ccgtgaccat tgaagtggac gtaagccagg
    12541 gcatcaactt ttgtatcgta ggattgccag actctgccgt taaagaaagt caacaacgca
    12601 ttgattctgc cttgcgtgcc aatgattaca aaatgccagg ccgcaaagta actattaaca
    12661 tggccccggc tgatatccgc aaggaaggct ctgcatacga tttacctttg gctataggca
    12721 ttttggccgc ttcagaccaa ataacctccg acaaactcga taaatacgtg ataatgggag
    12781 aactaagcct cgatggaggc ctgcaaccta ttaaaggcgt gttgcccatt gccataaaag
    12841 cccgcgaaga aggttttaag ggttttattt tacccaaaca gaatgcccgc gaggcagctg
    12901 ttgttaacaa ccttgatgtt tatggagtag aaacgttgag agaggtcatt gactttatga
    12961 ccggccaaaa agaactggac ccaatggagg tcaatacccg tgaagagttt tatgccaatg
    13021 tttccaatta tgacgtggac tttgccgatg tcaaagggca ggaaaacgtt aaacgggcat
    13081 tggaagtggc tgccgcaggg ggacacaaca ttatcctcat cggcccaccg ggagctggta
    13141 agactatgct tgccaaacgt attccaacca tcctgccacc gctgactttg catgaggcat
    13201 tggaaactac caaaatacat tcagtagcag gcaagatgga tcgcaactcg tcccttatga
    13261 cagtaaggcc ttacagaagc ccgcaccata caatatctga tgtagccttg gttggcggag
    13321 gaacatttcc tcaacccggt gaaatatccc ttgcccacaa tggcgtgctt tttcttgacg
    13381 aattacctga gtttaaacga gctgtgcttg aagtaatgcg acaacctttg gaagaccgta
    13441 ttgtcagtgt atcgcgtgcc aagtttagcg ttgaataccc ggcaagtttc atgcttgtag
    13501 cctcaatgaa cccttgtcca tgtggttatt acaaccaccc cgaaaaagag tgtatgtgtt
    13561 cgcccggtat agtgcaaaaa tacctcaacc gcatctccgg ccccctgctc gaccgcatcg
    13621 atattcatct ggaagttgtg ccagtgccat ttgagaagct ttcagaaaaa cgagccaacg
    13681 aaaccagcag ccaaataagg gacagggtta ctactgcgcg caaaaggcag gaagaacgtt
    13741 ttgccggcca aaaagggact cactgcaacg cccaaatgtc ttcaaaacag cttaggaaat
    13801 actgcgaact ggactctgcg tcgcaagaat tgttgaaaaa tgccatgcaa cgccttggcc
    13861 tttcagccag ggcatacgac cgcatcttaa aggtatctcg caccattgca gaccttgaag
    13921 gaaaagaaaa catagaatcg caccacctgg ccgaagccat acaataccgc agcctggaca
    13981 gggatggatg ggcaggataa acaaatatta aaaaaagaat atgaaactta aaataacact
    14041 ttttactttt tttgtctttc ttcatttgat gtttattgga caaaaacaat cagttcgcat
    14101 aggcccacca tgtcccaaac aacaatatga acctttattt gtcatagatg gtataagaat
    14161 acctccaagt gttggctcca agttcatgga tgaaaaaaaa gaaactattg atagtctttc
    14221 tcttcaacca gactcaatat ttgattgcaa tggaaaagta acaaactttg gtatagttcg
    14281 gatttttaca aaagattcaa taaatacagg agctaaaaag attcaatttt taacagatta
    14341 ttacctgaat taaaaacttt tgtttcaaag ttgattgtta acaacaatgt tgttgattgg
    14401 gacgaaaaaa catatcataa attgataagc cttaaacctt gtgatatatt atcagcaaag
    14461 attcttaacg aaaaaggtaa tttttgcgac agaatacttg aaataaaaat tttttaaact
    14521 taataacatt gccatgaaac ccagattaat catcatcgcc atagctttag tagctatatg
    14581 caaaacttca tttagccagg ttacagaagc tgaagataag ctaaaggtcc aaacaaaaga
    14641 cacattacaa ggttggcgca ccggaggcgt tttgggactt agtcttgcgc aaacctcatt
    14701 gaccaactgg tcggctggcg gacaaaactc catggcgggt aatggcattc tcagcttttt
    14761 tgccaattat aagaaagata aaacagcgtg ggacaactct ttcgattttg gttatggcct
    14821 tctaaaacaa ggaacagaca aggattataa aaagaccgac gataaaattg acattttgtc
    14881 caagtatggt cacgaagcat tcaaaaattt ctattatgcg gcgttgttca acttcaaaac
    14941 acagatgaca gccgggtata aatatccgac ggacacaaca aaagtgaaaa tatctgatct
    15001 gtttgcccct gcatatgttg ttggggctct tggtatggat tataagccta atgcttatct
    15061 tagcgttttt gccgcccctt taacagcaaa aatgaccatt gtaaataatc aagccctcgc
    15121 tgatgccgga gcttttggga tgaaagatgg taaaaagtcg aaaaccgaat ttggcggata
    15181 tgtcaggttc atttattcaa aaaatgactt taaaaatgag gctttgaaaa atgtttcgtt
    15241 cacaaccaag ctcgacctct tctccaatta tgcggaagac cctcaaaata tagacgtaag
    15301 ctgggaaacc caaatcgctt tcaaggtaaa caagtatatt tctgtaaatg tcaataccca
    15361 cctgttatac gatgacaata tcaaaaccat tgaaaaagat ggcataggtc aggatgttgt
    15421 gaaaggtgct aaaatacagt ttaaagagat tcttggagcg gggttctcat ataattttta
    15481 gctttatatc aaattcttat tactgtgcaa taacaaacct cacgctaaag ccaaagtcgg
    15541 tggcaatagt cataaatgag ctggatatat atggattggt aagggccctg tcgtagaaca
    15601 gcctaacagt aagcctgtcg ctaagctgat aatcagcagt aaaggaaatc ttaacattag
    15661 tattaccctg cgtcaaagaa ggagccactc catcatccag ttttcgcatc acgttcatgt
    15721 cctgacggat agaaacatct gcccgaagat cgaggtcgct cttgatagga ctggcgatag
    15781 cccctaaagt aagcatggta aggggtatat cggggaaccg ataaccggcg ccaaatacat
    15841 actcttttcc ataactttca attagcgaat agttcgatgt acctaacatc agcgaacggt
    15901 tctttttgta ttcagctcgg gcagtgatat tgttttttaa ggttacgtca acaccaatca
    15961 gcgggctgaa ttgctcagta atagataccg aaccaacatc aaattggggg acataaaacc
    16021 cggtatttct atcacgaacg tagctataat caaccctcgt atcatatttt gagttggttg
    16081 tatatgaacc aacattatac gttgccacat aagcatgggt aaggtttaca gattgtgcat
    16141 attcgcctac aataggaata tcaattaatc cgtcataatt gacacgccag ttaggcctaa
    16201 tcatgctcaa tacagggaac aatgccaatg tttttgaatc aagcccatat cctccgtaag
    16261 tagaaaggaa tgccggaatt aaggcttgtt ggattttgcg attaaaacca ggttgcgtgc
    16321 cagtatctaa actattgcca tgtctggcaa ttatttcctg gttgttttca tttaattttt
    16381 gggcagcagt aatagccgct tctttaaact tatcgaaata ttttgaattg gcataattgt
    16441 ctttagtcat attttcccat gagccaaggc tgatgatggt catgctcata ttgcctgact
    16501 tcatcgggcg tgagaactgg ctgaggtaca gctgaatagt atcgctaagg ttgccggtat
    16561 aaagatatgc agcagatata ttttgggtaa acgaatggtt gcctgtcagg tcaacccgga
    16621 gccccctgaa aggctcgtaa tttactcgca cattaacaga ttgggtcctg tttttcaaga
    16681 gtgagcctga taataattca ttatttgaaa tccattcctt gtgtaccatg cgttctacaa
    16741 gagaaggagc ctgttcgccc ataacaaact gccatcccgg ggcataatcc ccgctcatgc
    16801 ctaaatattt tgaaggacga atatatcccg gcaaagccgt accttcagtc tgggtataag
    16861 atacagatat ggtttttaca cccgttgcaa gcctcacaac gttgcgggca agaaacatca
    16921 gcacatccgg gccagcttct actttgcctt ctaccacaat cttggcccca gttacgtctt
    16981 tttcaggggt aaaaataagc ctgtttggcg atgccacttc catagtacct tttacctcgt
    17041 tgcctttgct gtcagttacc ttaatggtta catcttcaga acccagcttg tgcgaaatgc
    17101 ttttagcttc tccggctttt aatgaaaccc cttctttttc aaaagaaacc gttttcattt
    17161 ctttttcttt ctccttttgg gcaccttttc cacgtctgtt gcctgcattc aaatccctga
    17221 ggaaagcaac tttattatac aaagtattca ggtttagtcc cgggttgatt tgtatgctcc
    17281 tcgaattggc aatattatgg ccaacttcaa cgattgcacc ttcttcagac ttcataaatg
    17341 gctggcgttg ccattcgtaa gtgccggaat accttgtaga taagcttgtc cagtcaagta
    17401 acggcaattt attgacaggt acagtatagg taatattaat ctggtgctta taattaatgt
    17461 ttcggcctcc ttctttaatc tgcttccaca cgtcatagcg cgggtcattt ttatactgtt
    17521 tgtccattgt ttcgtcccac tcttcaatac gggcagcgtt tgtagccgaa aaatcaactt
    17581 tgatagccct ggacaaatca tagcggatat cgtacatacg gttccacaaa aagctttgat
    17641 cgatagtctt gggcaattca tagctgcggt cgttaaggtt tttgagctgc acctcatgga
    17701 aatcatgggt catattggtc ctgaacgaaa tgctggtagg ggcataataa aaattgatat
    17761 cccttatcag ttggaatgcc tttttgttca ggaacgaaac tttcttcagc ggcatgatat
    17821 ttttgggacg gttattgaaa atataattga agcccccttc atggtgataa tagtttttat
    17881 actttgttgc ataattgtag gatttggttt cgttgtaccc gtaacttacc gagaagttgg
    17941 ccgggtcgta aaagcgcggg gtcttacttg ccttgttgac ctttacattg gtgaagctga
    18001 aactcctgcg caatgtatga tcaatcgctt cttttctgag cttgtctttt tcagcctgcg
    18061 ttttagcgtt gtcaatagca gcctgcaaag gtatatcagg gtcataagga ttatactgtg
    18121 gcaatatatc tatttgagag tacgatgcat aaaaagggat acgaacctgg gctttttcgg
    18181 ggaaaaactt accaagctca aggtctgttg aagcatctat ttggttggta gtttctttag
    18241 ccctgtcatt tactttcttt tcaatacttc caaaaccagg ttgcattgta gcccctgcaa
    18301 tacggatatt cccaaagtcg gcaagcttaa tctgggccct ggcattggca gcccatccgc
    18361 cctgatcact aaaatcagta agtcgcaatt cgtccaacca aatttcggcc gattttggag
    18421 cgccatcatt agagtatttg ttattggcct cagacgggtt cctgactcca atcatgatgg
    18481 tgcgtatatc acttaatgac ggattgccgc aaatgtacat gttgttatcc ccatcaggtt
    18541 ttacatatcg cttcaataaa gaaacatcag catcattacg ttcctgtttc agccgaagga
    18601 atttggccaa ctcaatatca aactggtttt tagcgggcca catggcatcg cggctaatat
    18661 ttgtcccaat ggggctaacg atcataggca cttcatactc ataatagttt tctttattgt
    18721 cagtaccaaa acggacaaat acagtcacat cgccattttg aagagcctcg ccgttcagcg
    18781 gctgggcatg ggtgtacatt ttcagtctct tgtattggcg catgtccaca tgcccatttt
    18841 tgaacactgc cctggcatcg tcatcggcaa ggttctctac tttaagcacc atagattgct
    18901 cattctgcaa ggtgtattgt gagttggtag ggtcaacagg ccgtgcatct tcagatggga
    18961 tatcataaac atccccattt tcttcaatat taattgattg caagtcaaag ttactaacag
    19021 gggcctgtcc gccaaatcct tctgagtttt gcaaaaggga gccattgtat ttacgccatt
    19081 cacttctgac cagatccagc gtagcaaaac gaaatgtagt tttttggtta aacccggtaa
    19141 gataaatgcg taaaaatctg atagatttga aatcttcaat agtgccaaat tttcttgtaa
    19201 aatcttcaat agggatttta aactgcaccc aacgtgcttt aatatttcct ttcgaatagg
    19261 tagtagcatc aacaacataa ttagtcccct tcaccaggtc attaggcctc atgctaaccc
    19321 tgtattcaaa aaagttttcc ttatcgctga gggtattgtc atcattgata tcttccatgt
    19381 cgggggtact gtaattgctt tgtgaaaaat ccttaccggc ttcaggggag ttgttttcca
    19441 taccattata aaactcgtac cggctgacaa tatctttatt attaaaatca gcattcctga
    19501 aatatttaaa gtcatcgcca gaagggtcat ccataattgg cgtcccttga aacttagctg
    19561 caaatgccgg tgatattttt gcttcatctt cgtttgacaa accatctaaa ccaacatcct
    19621 gatattttct aatattattc cctgtaacaa aagctttggt tatagggtca gaatcgggca
    19681 ctttccccca aactgtttca actactttgg atgaatcgtc tcctggagtt ggtaagccct
    19741 gttcaaaaga catttttccg tctttcagga tgtcttccga aatatctccc agttgaaaat
    19801 acatatcccc cccctgattg ttatttgtag agtcattata aaaaggatcc atgacccaaa
    19861 actggatata cccgatgttg gccgcttcaa agtcattggt ttgcaaagcc ctcatcatac
    19921 ctccccaacg agcctcgggg ttagtaatga agttcccatt ttggtccaaa tctgttgtgt
    19981 taaagttgta tgggccacgt ttattgggat taaatgtaag gttcaatata tacatattgg
    20041 ttggtacgcc attgacattt tgttttttcg ggaatagctc tgtttccaat atttcacgag
    20101 agtcgttggc atttagcata gaatctgtaa gcccggcagg agtaagagaa ctttttcggt
    20161 aaaaagattc gtctatggtg taaaatgaaa ggcgtgccct atccctgtta taatcgaggt
    20221 tgttgaaata gttgccttca acacgtgtct gcggggtact cgacaaagtc catccggtaa
    20281 tatatttcag gtcatatttt gtttcgctgg cctcaaagtc atctatataa gcaacgccct
    20341 ttttcccaat cttgctagaa gtaccaggga taagctgggc aaattcggca ttaaccgtaa
    20401 tagaggacat ttctttagtt tgtagcaatg gcagcttatc aaccatagtt gttagaaact
    20461 gtgaattagc acggtacgaa gcgttcagcc cccagatagt gttcgatatt ggttcatctc
    20521 caatgttgac tttgctggta agcggcgttt cactaaggtg taaaagagta cctccaatgt
    20581 caaaattatc gttcacctta taattaaggt gtgtacctat catggttttt gtctggaaat
    20641 tgaaattggc attgttttct aacgaaactt taataggctg ccccgaagac atgatagcac
    20701 tgttggttat cttaaccgta cccagagcat aatccaccac gtaatcctgg ttttcaacta
    20761 atggcatgcc accagaggtc acttttactg aacctttggg aatgttcatc gcattgaggt
    20821 atatttccga agaagaggcc gacttgtagg taccttttaa cttgaactta tttttttccg
    20881 aaacatcctc tgccttggtc tttgtagagt cgtataattc gtagtaagta tatttactcc
    20941 atgtgaggga atctgttacg cctcgtttta taaacctgtc acggagcccc cggccaaaag
    21001 gttccaacat gggaaaaata acccgaccgt aacgtttgtc aatggttact ccatcaataa
    21061 agtcaaaaac cccgtctgaa ttgccattca tctggctatt ggtattgtcc aaacctaaaa
    21121 atttgagcaa tgcaatacta tcaatgcgtc cggcaggctc agggatataa ctcacatccg
    21181 acccgttttt attgtcgtga taaagcacat tcaggtaaaa atcatctttg ctaatctcat
    21241 aagcattgag gttgtaaatg tttttcatca tcagatccca agtcgaaagc tttggcgaca
    21301 atgttgtccc tttcaaaagc tttaacacca aaggcgtttt agatgatgta acttctgtgg
    21361 acaaatcccc taccttatag gtatagccgt tgaccatata ctcgtaagcc acaccaagta
    21421 tttcgtcatt gttcaacgac atgttaagcg aaatataacc aagttttgtg tcaacagaat
    21481 attcgctgct ggtaagtttt ctggcgcttt gggttttatc gtagtcgcgt ccatcaacaa
    21541 gccccaatgt tctcaggctc tcatcatccc tgttgcttac aggggttttt acaatgttgt
    21601 ataaagtatt agcaccgttg gatggccagc ctgtattatt aggcgcaatc tgaagagatg
    21661 cagcgttgct cagtacagag gggttagttt cgcccaaatc ggtaaatgca taaacaggcc
    21721 tgacatcttc taccttgccc ctggttttgt tggtcaccca aacttctatt ttagtgatgg
    21781 taattggaga agttatttgc ggtaatgatt tatgtgcttt gtcaaagttt cctcgaaaac
    21841 tatgcccaag gaaaaagtgc ttattggcct cgtattcagt agatgtaatt tcaaaagggg
    21901 taacctgagc gcccccttgc acctcaacca cctgtgtctg tcctttttgc tgagacagta
    21961 ctgtggtaac atttagtttt ccaaactgca attcggtttt gataccaaac aagctctggc
    22021 taccagtaat aagcgtaccc ggcaatggca atgtaacgtt tccggcttct atttttctga
    22081 caatatcgtc ctcatcgcct tcgtattcaa tcttggtctt gttctcgaac tcaaaagaag
    22141 cgtcagtgtt gtaagaaacg cccagtttaa cgcggtctcc aatcgttccg gtaacattca
    22201 tctggatttt ttcttcaaag tcaaaagacc agacactacg ttgcgtttca ggaataatag
    22261 ggttattggt ggtatttaca ttgcctttga atatcagctc agcctcgcct tgtgggacaa
    22321 tagaaatagt actaccccca aatacttttt gaaacacttc gccaccaaca ttgaatgagc
    22381 ttccaagatt acgccctgta cctttcccac cgttggcttg ttgtttccaa tagtcgcgaa
    22441 ccgttttttg catctcgtat ttgcgatact cgcttggctc catatagtat ggctggcggt
    22501 aatcgaggtt gccaaccttg ttttttacaa catattggtt ggtctcaggg tcatactcaa
    22561 cttttgattt gaaattagaa gggttcttta attgaatccc ttccggctca cctatttcag
    22621 agtttacatg atcagggtct ttctttagct taaactgtgg gccttcagcc ttaaagttag
    22681 tatcatcaac tggttcttca tcatcttcgt attgagaggg tgttttttta aacacacgaa
    22741 aagaagcttc tgttgtcatc aacgcagccc caataaacac caatgtaata gcagtaaaaa
    22801 gatattttga aaacttagtc aaacctgtac ccaataaagt ttataacact tttaacgcaa
    22861 gcttaatgta ttcttccact gaagcctgct tattttcgcg cgaaatttgt gccagcgctt
    22921 tttcggcagc ccctttttga aagcccaaca ttactaaagc agataacgct tcatctgccg
    22981 aagtattgta ttgcggcaat aaattttgtt ccgagcctgc aattttaatc attttatctt
    23041 taagttcgac gatcatccgc tgtgcagttt tcgccccaat gcccttaatg ttttgcaaag
    23101 ccctcacgtt ttccttgcta atggcattgg caacctcttc agcagtaagc gaagacaata
    23161 tcatgcgggc agtattggcc ccaatacccg aaactgaaat caggatacgg aaaacttcac
    23221 gctctttcgc ttcataaaag ccatacaaag tatgggcatc ttcctttatt tgtaaatggg
    23281 caaatatacg agcctgagta tttacttcaa gcttagaata agtatttaac gaaatgtgca
    23341 tcacataacc tatgccattg ttctctacaa taagatacgt aggtgtaagc tctgtcagtt
    23401 tgcctgttat ataatcgtac ataatgtttt taggcgtaat acgcttgtta tatgattttt
    23461 actaaaatta ttcctaacaa aatgaggcta aaattactat aaaaatcaga aataggaaac
    23521 ctcagggcaa attgaaaaag aaatatttaa cacacttgtt attatgtgaa aagtttttat
    23581 ctttgcagtc cattttttga ataattgtaa caaaataaaa gcattaagaa atgtacgcaa
    23641 tcgttgacat agctggtcag caatttaaag taacaaaaga tcaaaagatc tatgttcacc
    23701 agcttgaggg gcaagaaggc gcccaggtta gttttgacca agtccttctc atcgacaatg
    23761 aaggcgatgt aaaagttgga atgcccacag tagaaggggc tagcgtaaaa gctactgttt
    23821 tagcaaatgt taaaggtgac aaagtgttgg ttttcaagaa aaaccgcaga aaaggttacc
    23881 aaaaactgaa cggccaccgt caatgtttca ctcaaataaa aattgatgaa atagttggcg
    23941 ccggtgaagt tgctgctaaa ccaaaaagaa caacaaaaaa agctgctgcc gctcccagcg
    24001 acgaagctca agcataattg aaaagttaat aaaattaaat agtcatggca cataaaaaag
    24061 gtgtaggtag ttcgaagaac ggtcgtgatt cactgagtaa acgacttggt gtgaaaattt
    24121 tcggtggaga agttgtaaaa gccggaaata ttatcgtacg tcaacgtgga acagtgcaca
    24181 atcctggaaa taatgttggt attggtaaag atcatacttt attcgccctt gttgatggca
    24241 ctgtaagctt caagaaaacc cgcgataaca aatcttttgt ttcggttatt gcagccgaat
    24301 aaacaaattt tacaatatct gactcttgca gttgtttttc ttgcgaaaaa taattgtaag
    24361 agttttttta ttccatcact ttaaacttca acaccatgtt gactttacaa gttataagag
    24421 aaaaacgtga tttcgtcctt gaacgcctgg ctgttaaaaa cttcaagcaa cctgaactgg
    24481 ttgataaaat cattgaactg gacaaccaac gcagggcaat acaacagcaa gctgatgcca
    24541 gccaggccga aatgaacaac ttatctaaag aaataggtat ccttttcaag aacggcaaaa
    24601 aagaagaagc tgatgcggct aagttgaaaa ccaatgagct gaaagaaaaa ataggacaac
    24661 tcaccgaaca acttacagtg gttgaaaaag aactccagaa tactttggtt ctattgccca
    24721 atttgcccca tacaaatgtt ccaaaggggc acagcgcaga agataacctc gaaatccgta
    24781 aagggggcaa tgtgcctgct ttagccgatg atgcaatgcc tcactgggat ttggccaaaa
    24841 aatatgacat catcgatttt gaacttggtg taaagattac cggagccggg tttcctgttt
    24901 acaaagacaa aggggccagg ttgcaaaggg cattgattaa cttcttcctt gacgaagccc
    24961 gcaaagcagg atatattgaa atgatgcctc cgctgatggt caacgaagac tccgggtacg
    25021 gcactggtca gcttcctgac aaggaaggtc agatgtacca tgcaacattg gataactact
    25081 atcttatacc cacagcagaa gttcctgtta ctaatattta tcgcgatgta atattgaatg
    25141 attctgattt tccgataaag aatactgctt ataccccatg tttcaggcgc gaagcaggtt
    25201 cgtatggcaa agatgtacgc ggccttaacc gcctgcatca gtttgataag gttgaaatcg
    25261 ttcagatcca acacccagat aaaagttatg agactttaga gcagatggtt gaatatgttg
    25321 aaagcctggt taacaagctt gaattgcctt accgtatcat tcgcctttgt ggaggcgata
    25381 tgagctttgc ttcagccatg acatacgact ttgaagtatt tgctgcagct caaaaacgct
    25441 ggttagaagt aagttcagtt tcaaattttg agtcgtttca ggcaaaccga atgaaactgc
    25501 gtttcaaaga agccggagaa aagaaaacca ctttagccca tacgcttaat ggcagtgcac
    25561 tggctcttcc tcgtattgtt gctgcattgc ttgagaataa ccaaacagcc gagggtatta
    25621 agctccccaa ggcattagtc ccatactgtg gttttgacat gataaattaa tcaatactct
    25681 tgatatgcaa aagcctgata acgaaaatgt tgtcaggctt ttttgtataa atttatcttg
    25741 taaatattat atttactcac attctgttaa cgaaaacccg accgctttaa gatctgtcca
    25801 aaatgcagga tatgatttgc gcactacatc aacattttca atgattacag gaatacccat
    25861 caatgctatt ggggcaaaag ccatagccat acgatggtca tgataagttg gaatgcgtat
    25921 gggctcttta atagctgcaa gagagttacg tttgccatcc caactgaggg ttccattatt
    25981 ggaatattcc aaaacagcac caaacttgcc taattcattc tgcaaagcag caatacggtc
    26041 agtttccttg atacgtaaag tttgtgtacc ggtaaattta aaagaaatgc ccaacataac
    26101 gcaagtaacc gccaatgttt gaacaatatc agggttttca ataaaatcaa attcaaaatg
    26161 agcaggaata tcagtcgatt tagaaagagc tattccatct ttattatatg cagtctcaat
    26221 tccaacgtgt ttaaatactt cagcacaacg agcatcgccc tgaaaacttt ccttatataa
    26281 cgaagttata gcaatatcag gctttttggc taatgccgca atctggtacc aatacgaaac
    26341 gccactccaa tctccctcaa cagtaaaatc cttcattttg taagtagctt ctgggacaat
    26401 aagcagatta tcctgttttt tgaccaaaac tccggctaat ttcattaaac ttattgtcat
    26461 ttctatataa gacgaagaaa taacattctt ttcaagctca agctcaagcc cccctttaaa
    26521 tgtaggggca accatcaaca aagcactaat aaactggcta cttatgctac tatctatagc
    26581 tattcgcttt ccattaagct gttttcccct aatttgcaaa ggaggataac cttttttttc
    26641 aacatattca atatctgccc caagattttg taaagcttca acaaggcttc caataggacg
    26701 ttctttcatg cgggcagatc cagtaagtat tttattgcca ggctgcgaag caaaataagc
    26761 tgtcaaaaag cgcatagaag taccggcgtg accaatgtca ataatatcag attcagaaac
    26821 aagacctcgt tgcaaaacaa ctgtatcttc actatctgac aagttattga tattaaattt
    26881 ttctttacac aaagcttgta taataagcaa acgattggca atgcttttag aggcaggtac
    26941 cttgataatc ccctctacat tttcaccttt aaattctacc ttataattca cttttacatt
    27001 tttattggat gcaaagatat tctattgatt caaaaatgtt tataaacttc aagtctttgt
    27061 gtctttgcgt gtaaaatttc aacataatca taaaaagcat tacattagta caaattttaa
    27121 gtaagtatga gaatagaaaa ttctgccttt gttgtccgcg aacgcaagct aatgtacctt
    27181 tcaactatca gcctgaccat attgataccc attattttcc tggccgattt ttttaatacg
    27241 cctgtattgg ggctcaacag agaactttat attattggta ttgccttact ttatatattg
    27301 ttgcacttat acagatattg gctcaacctc aattttattg aagctgctac cgaagacaat
    27361 aaacttgttt tcagatatta ttcattgcgc ttttttagcg ccaaacacaa aagtattgaa
    27421 attcctttag ccgattttat tggttttgaa ataaatcagc cattcaaagg ccttaaaaaa
    27481 agtttgattc tttttcaaag gatgaaaaac aaaaaagcaa aataccctcc tatctcaatt
    27541 tcagctttaa caaaagagca aatagccgaa ttagaaacct ttttaaaagg tttgtctaaa
    27601 agcatttaac atttatggac aattcactac tccatgctat agctttaacc atgattccag
    27661 gcattggctg catgactgct aaaaagctca ttgcttacac aggaagtgtt gaaggggttt
    27721 tgaaagaaaa aaaaagcaat ttgcttaaaa taccaggcat tggcgaatat gttgcagatg
    27781 agatactcaa atcagaggtg atgacaaaag ctgaagccga acttgccttt attgacaagt
    27841 ataacataaa agcttggttt tacttagacg acaattaccc ggcccgactg aaacattgcc
    27901 ctgattcgcc cgtagtgctt tattctaaag gcaattgtaa cttagattcg cccaagatga
    27961 ttagcattgt aggcacacgt aaagccacag attatggaaa agcattttgt gagaaactaa
    28021 tttttacctt agctgagaga aaacatgatg tagtcatcat cagcgggtta gcttttggca
    28081 ttgacattac ctctcataag gcagctataa aagccggatt gcctacaata gccgtaatag
    28141 gtcacggcat gaagacagta tatcctgcag aacacgataa atatgcccgt gaaatagcca
    28201 aaaacggggc actggtaacc gaatatacat ctgataccaa agcagacagg gcacttttcg
    28261 tacgccgcaa tcgcattata gcaggtttat cagatgctgt aattgtagtt gagacaggca
    28321 aaaaaggagg ggcacttgcc actgccgaat ttgccaactc atataaccgg gatgtatttg
    28381 cccttcctgg acgcattgat gacaatcact cgcaaggatg caatatgttg ataaaacaaa
    28441 acaaagcagc gttgatagaa tcagccgaag atattgaata tcttcttgga tgggcttcag
    28501 aaaatgaaaa caaaccaaag gttatacaac gtaacctttt tgtagagttg acagaagaag
    28561 aaaaagtagt gatggatgta ttaaatcgcc acgactcagc acatattgat attattgggg
    28621 ttgaaagtga aatgccagta agcaaagttt catcaatgct tttaaaactt gaatttgatg
    28681 gaatggtaag aagcctgcca ggaaaaattt accgaaaggt ttaaagatgg tgttctctat
    28741 aatactaaga atttgacaca aacaggtttt gggactgtta tgtcaatgcc ggttgaaatc
    28801 aggtctccag tagtaggatc aattttaaaa accacaatat tgttgctgtc catgttcgct
    28861 acaagtaaga atttccctgt cgggtcaatg gcaaaattcc ggggtgctgt acctccgcat
    28921 gcaacatgct ttatcagttg cagcgttcca tccgtctgat taacggcaaa aatggcaata
    28981 ttgttaagtt cacctcgatt ggaaacataa agaaaccggc catcagcagt aatgtgtata
    29041 tctgccgagc caggataaac agcagaaact gcaggcgctg aagaaatact ttgtatcttt
    29101 tgcaaagaac catcagcagt tttgattgtt gatacttcta taatcccctt taattcatgc
    29161 aatacataaa gcatgtgttt ggcaggatgc acagcaaaat gtcttggtcc ggccccagct
    29221 tcagatttca acgatgtcaa aagctttaat ttgccggttg ttgtatctaa agaaatagta
    29281 tgtaccttgt cgatacctaa atcggtagca taaacaaatc cgattgatgt atcctgatct
    29341 atcatgtggg catgagggcc ttcctgccgt atagcatttg tactttttcc tttgccatgc
    29401 aggacagaca atacctcagt caacgttccg tcaaccccaa ttccaaagga tgcaaaacta
    29461 ccactgctgt aattagccgc catagcgtat ttgccagttt tgtcaatgct gatataacag
    29521 ggcgaattgc cagaagaggc aactgaattg atttctacca acgatatatt tgttgtgtct
    29581 attttgaaag aggttatttt gcccggccac gattcattca cggccaacag ccatttcttt
    29641 gatggatgga tggtgagata cgaaggattg tctgtattgg aagacacacc aacaaactta
    29701 agcgacccgc tgtcagggtt catctggtaa atttggatac cagtagattt cgcaactccg
    29761 gttccatcag tataagtgcc gataaataca tagtaacggt tcatttttga aagttttgaa
    29821 gtgtctgtaa aaattgagcc tttggctgct ggggttttat catcttttga acagctataa
    29881 gacagtggtg tagctaaaaa gatggataaa actgctacaa tgccaaaact taacgaagaa
    29941 gtttttctca taatatactt tttaaaaaca aaccctgcat ttactaaatc ttggaatagc
    30001 aaaggcaggg cgttttttat tattaattta accttattac aatattacaa ttcctgcttg
    30061 aaaattacgg ccaatagtag ttcattaaca agcatttaat atttgtttag ttgagaattt
    30121 tcaacctggc aaatttcaac aggagcgttt tttctccatc catttcaaat accactgtag
    30181 ccttattgtt aggagctgat ccttctatag atactaccat ccctggccca aacttttgat
    30241 gcagcacagt catgccttct tttatgctat tggggtcatc aggttcaaaa ttttcaggta
    30301 tagcaacagg tttacggtta acttcctgta ttcgggtaag cgtgcgcctt acttcattta
    30361 ccgctgttga agttgactta ggttcagcta ccgaacgcag gtcgcgagaa tatgctttag
    30421 tacgaggatc tacaggcatc ccatcggtag tagccttagg agtatcatac aaatcgcgag
    30481 cccttgaggt gtcaagaaat ttctcgtcca catcgcggat aaaacgacta gggacacaga
    30541 aggtaggcga accatgcttg tacctaaagt tagaatatga aagcgtagcc tttacagcgg
    30601 ccctggtaat agctacataa aacaacctgc gttcttcttc caattcagca ggcgaaccta
    30661 ccatacgttt tgaagggaaa aggtcttcct caatgccaac cacaaaaaca taattgaatt
    30721 caaggccttt agatgcatga atagtcataa gggtaacttt ttccctggaa gtttcatctt
    30781 cattatcagc atcagtcagt aacgaaatgg tttccataaa ctgattcaat gtaactattt
    30841 gattttcctc agcattatca tcaataaact tctgaatact gtttagcaac tcgtctatat
    30901 tttcaatacg gttaagactt tcctgtgtat tctcttcttt caaagcattg agatatcccg
    30961 aagcaactgc aatatgcctt gcaaggttat aagcatcggt attgcccacc atttcagcaa
    31021 accctttaat tagtttcaca aagccttcca gtttagctaa tgtgccttta ttgaaattga
    31081 tactgccaag catttcagag tttataacat tccataagct tgtctgattt tcattagcgg
    31141 aagtttcgag cttttcgagt gttgtatcgc caattccgcg ggcaggttcg ttgatacaac
    31201 gtttaaaagc ttcgttgtca ttggggttta ttactaacct aaagtatgaa gtcagatcct
    31261 ttatttcttt gcgttgatag aacgatagcc ctccataaat cttgtaggca atgcccattt
    31321 tgcgaaaagc atcttcaaat acacggcttt gagcgttagt acggtacaag atagctattt
    31381 catcaaggtt aactttatct tcgcgcttca gtgtattgat ctcattggct atactgtatg
    31441 cctcttcaat gtcggtagca ttagaaatga cattgaccag ctccccttca gcattttttg
    31501 aaaaaacgtt tttcggaatt tgctgtgaat tttttgcaat aacactattg gcagcattga
    31561 caatattttg cgtagaacga tagttctgtt caagcttaaa aagctgatat tccggatagt
    31621 cgtttttaaa atttaagata ttctcaatct ttgcaccacg aaatgagtag atactctggg
    31681 catcatcgcc caccacgcaa atgtttctgt gattggcggc caatttttta ataataagat
    31741 attgggcaaa gttagtgtcc tggtactcat caaccagtat ataacggatg tagcgctggt
    31801 atttggccaa cacatcagga aaatcgcgga acaaaatgtt ggtattcaac agcaagtcat
    31861 caaaatccat agcatctgaa gccttacaac gtgcattgta caggcgatat atttcagcaa
    31921 tgtatgataa tttagcggcc ttgtccctct cgataatttc agcagatgca gcataaacag
    31981 caggcgtgat aaggttattc ttagccatag atatgcaaga ttgcactatg gcaggcttgt
    32041 aattttgcgt atcaagcgaa agacttttta ttacagcctg tatcacactt tttgagtcgc
    32101 tgctgtcgta tatggaatag ttggtcgaat aacccaaaga agctgattca atacgcaaaa
    32161 tctttgaaaa gatagaatga aaagtgccca tttgaaggtt tcgtgaggct tcctcgccta
    32221 caagctttgt aatacgctct ttcatttcgc gtgcagcctt attggtaaag gtaagagcaa
    32281 gaatagagcg ggcatcaatg cctttttcta acagatgagc aattttatag gtcaatacac
    32341 gtgttttgcc cgacccggca cctgcaatta ctaatgatgc accattggta cactcaacag
    32401 cagcacgctg gctttcattc aactggttca aaaaatcact cacaagattt cattttagtg
    32461 gctcaaaaat acttcaccta tatggctctg cattaatatt tttatgatga aatattaaca
    32521 aacgtcacaa gggctgtttt ttttacccat ataaacaatt tttgccgact tttgtggtta
    32581 taacaatgct aaacattttt cttgaaaaag aaacagacac agttagcagt atttcaatat
    32641 atttgccact tatttttaaa aattattttt gcaaaccaaa ttgaaacaag tatcttataa
    32701 aatattattg gtcataagca ttgtgttttc tgcctacaca gaagggtatg cccaactgta
    32761 ccatgtaggg gccaataagg acacattgat taattatagg aaaactgctg attttaaagc
    32821 tttctacgcc aaagtatttg cttataatgt tgatattaaa gcaaaagaac atattggaaa
    32881 aataaaagcc atttcgccag accgccagag cggttggact tttaaatggt ggacttacaa
    32941 ctttgctgaa agaattttaa aagacacctt gtctgtttat agcaatatag acagcatgga
    33001 gcttgagaac cttccaacag gctgctacaa ggtacaaatg tcaaaaggaa atgttgatac
    33061 cagcttctat gcctggattg ttatcaaccg ttcacgtttc cacttagtaa aagatgataa
    33121 aggatatatc aaatactttg aagccacttg ttttaaaaca gatataaggg ccattccctc
    33181 tataggagaa aaatttcctg atagtttaag gtatgtacac tctgacaact atgtccttcc
    33241 taatccagaa gactatcaat acaatgtttt tcagaataca cctcgtatta ttataaaagg
    33301 aacagatgga agcatctata cggcagataa aaacaaatat agcatcacaa atcccccgtc
    33361 aaaagatact cgttattttg tcgagttttc tgataagcta aacaccttga tcagagacac
    33421 tgttgtttac aaatccatta ttcctaaagc tgaatttagt cttttcattt cggaagatac
    33481 aatgtcagag gtttctacgc gtttttacac tgaaaaagct tcaggagagg ctcctttgtg
    33541 ggcaaaagtg gttagcaaaa gccaaaatac caaccacctg acctgggtac tcgctgacag
    33601 catcaaagac cctgctgata gcgtttatta ttttcacaca gccaatgatt ctattaatac
    33661 tataagtgac attccttaca aatatcctta taaatacaag ctcatcctgt atgcccaaaa
    33721 tgaccgttgt acagattcta tatctaaaac tttagaagta gtcaactcaa aggttggaga
    33781 acaaggtgca aatgcattcc caaaagtatt taccccaaac ggggatggta aaaacgatta
    33841 tttttcgatc aaatcagaca ctatacgatc catccaattc cttcaccttt tcattgttga
    33901 cagccacggc aaaaaggttt atgaatataa aggcaaggta gcccagggat ggaaaggctg
    33961 ggatggaaaa acttcatggg gctcagacgc caagccgggt atatattatt attattttga
    34021 agctacaggt tgggggccaa tacctaaaac aggagtaaca gaccctaaac aaattaaaaa
    34081 ttctccaaat aaagtttcag gaaaaggtta tttttacttg ttcagagaca gcaacaacta
    34141 aggaacaata ataaaaggca ttatttttcc aaattgataa accggaattt gccctttaaa
    34201 atatgtttta tcgttgtcag caatcgaaaa atcaacattt acaggcattt tgtaataaac
    34261 gacattcttc ccctgcggca aaacctgagt tgcattgttt tctttttcaa taatcataga
    34321 aagtttaata gccttacctt ttttccccga aaagccattt atttcgtcga accaaaacaa
    34381 tgttgcagat gcgtcagtag ttaaatcatc tgaaggtgtg taataaaaag ttgcctggca
    34441 gcttgaagta gtggtttgcc ctgtaaacag catgaggtag cttctttcca tttcagcaag
    34501 ggcatttaat tgagcattga acatcttttt gttggtatga ggctcgtctt cgcccaaaat
    34561 tatttcatac tgcctttgcc tgagtttgaa aatgtcctgt gctgcttctt tggccctgtc
    34621 acgaagtgaa acagcagcag tgacgttctt tataattggc agacgatggg taaccgaatc
    34681 cctgattaca gtccgaaaag ttgtatctgt agtgggccta attataggct tgcgtaatat
    34741 ttctgaaatg atgttttgct tttctccaat atagttgtca gtagttgcct gtttaaaacc
    34801 aatagcctga gggcttaata taaaacctgc atcagcaata ggttgtaaaa accgcatttc
    34861 aaactttttg ccaggtttaa tcccaaccaa ctgcttttta tctggcacag taaaattaga
    34921 gactttaaca ctgtcaattc tccaataagt cctgttggtc ttcactatat cagttatacc
    34981 aagcatctct tcagcaaatt ggctgtatgg cccgcttttt tcattgattt gactataaaa
    35041 aacagttgct cctattactg ttttaggcaa aaaataataa ataaaagctt cttcagtgct
    35101 aatattgtta taatctacca aaactgcctt ttcaacatat tgagctttgg ccaataagcc
    35161 taatgaaagt atcccaaacg caaaaacaat ttttaacttc atattctttt ttttgaaaaa
    35221 acggatgagg tgcaaaaata ttgaaaaagg ccgaatctat ttactaatgt ttcgcagttt
    35281 caatttgttt aaacgccttg ggcagggcat tgataatatc attagccata aggctttcct
    35341 gacaaagctc ttttgcagca atatctccag ccaagccatg taaaaaaact cctgttatag
    35401 cagcatgtgc agcatcatac ccttgagcca ataaactgac aataacacct gtgagtacat
    35461 ccccacttcc ggcagtggcc ataccattat tcccagtcgg gttaaaataa caatgccctg
    35521 atggtaatgc cactatcgaa taatgccctt ttaaaacaat aataatatca agggatatgg
    35581 cattttctat gagcctggca agccgttcct cactattggc tgatgaacca aaaagcctgt
    35641 cgaactcttt aggatgcggt gttaatatag tattggctgg tagttcgttt ttcaactgag
    35701 gcttagacgc aagtatattc aatgcatcag catctataac aaggttctta gggcgacttt
    35761 ttatcaaccg cgaaaaagca gttttagtac tattagtaat accaaggcca ggcccaacag
    35821 caacagctga ataataacta atttcaggga tatttgaagt ataattatca cctttatcaa
    35881 ggcttagaat ggcttcgttg atggcaaccg gcaatatacc agccaaagca gaaggaacgt
    35941 gagaggtaag caatccacat ccgcaacgca atgctgcttt agcagagaga atagccgctc
    36001 cgggcataga cctgctgcct gctattatca gtgcatggcc aaatgtaccc ttatgcccga
    36061 actttaacct tttcttaatg tgaatatctg atgtttcggt caaaacatat tgaacctggg
    36121 tctgggcaac tgcatttttg tcaagtccga tatccagcac tgtccaatca ccaacaaatt
    36181 gggcattttc agcaaaaaag aaagacaaaa aaggaaactg gaatgtcagt gtatgttgag
    36241 cattgaaaac tgtttgccct gtattggcct taacatccat caatcctgta ggtatatcta
    36301 tagctatttt ataggctttt attttattaa gtttttcaat cagctcagcc aatctgccat
    36361 ccagcggttt gttcaggcca gagcccagca cagcatcgac aattacatca tcttctttta
    36421 ttgcaggcaa gtgttcttca atgttttcaa accttacata cacacgggta tttttcagcc
    36481 ttcccaaatt caacgctgcg tcaggcgaaa gcttatcttt tccagcgacc agataaacag
    36541 tagctaaata atcctggttg tcaagctgtc tggcaatagc caaaccgtcg ccaccattgt
    36601 tgccactacc acaaaaaata tgaaagtgct gatgcttccc aaaacgaacc attagccagt
    36661 gggtacactg ccaggcagcc cgttccatga ggtcggcact ttttataggt tctgattcaa
    36721 tggtcatccg gtcaatttcc ctggtttgcg cagatgtaag tattttcagc atggtaacac
    36781 tggttttaag ttacaattga aaactcctta gaagggcata ttttgtcaaa ctttgacaaa
    36841 gttatttttc ttattgcaat aatgttttaa attagcacct tagatgttat aaagtttatc
    36901 aaacaaaaat acgaaaaata tgttcaaagg acatcccaaa ggcttatatg tgctctttat
    36961 ttccaatatg ggcgaacgtt ttggttttta taccatgttt gctattctca cccttttttt
    37021 gcaagaccat tttggatgga acgaaagtca ggccagcatc ctgtatgggt ggtttatcat
    37081 gttgagttac ctgactcctc tgttgggagg cattttggct gataaattct ttggatatgg
    37141 caaggcggtg atattaggtc tcattgttat ggctttggga taccttgttt tatctcaagg
    37201 gaatggttca actccttata caatctatgc cggattgttt attgttgcat tagggattgg
    37261 atttttcaaa ggaaacctca atgtccttgt cggaaaccta tacaatacct cagaactaaa
    37321 gtcattgaga gattcagctt ttatcattta ctacatgggc atcaatgccg gagcactctt
    37381 tgccccttat gccgcagcat gggcaaaatc tttgttcctc cagcatgcag gctgggttta
    37441 tgatcctgca ctaccttccg ttttacacaa catcatagca ggcattgcac ctactactga
    37501 gcagttagct ttggtaaata aatacactaa ttcagccgat ttggcaggtt ttgcccacaa
    37561 ataccttgaa ttattgtcaa aaggatatct catggcattt ggactggcag caatagcaag
    37621 cgttgtatca ttaggcgtat ttttattatt taaaaagcac tataagtacg ccgattatac
    37681 atataaaaat gcaagtgctg acgctaaaga gaaagaactc accccgacag aaaccaggca
    37741 gcgactggta gcattgggat tagtattttt agttgtgata tttttttggc tggcctttca
    37801 tcaaaatggc agcaccctga cgttctttgc caggacatat accaacctcg aggtgggcaa
    37861 attctccggc ctgttttttt cgttgccttt cattgtttgt tttgtcattt ttatttctct
    37921 tttgccctca gttttcaaca aaaaaattgt agccatccga agaagccttt atggagctgt
    37981 ttctttagtt ttattgatgg ttttggctta ttttatctat acgcagccag ggcaaagcac
    38041 aatagaccct tcattattcc aggcattcaa cccaattttt atcatattgc tgggtccatt
    38101 ggtaatctgg gctttttcac ttctaaacaa acgcggggca gagccttcaa ctcctgcaaa
    38161 aattggcctg ggaatgttca tcacggccat tgcatttggc ttattgataa tcccgtccat
    38221 cggaatgcct gtggcagaga gcttacaagg tgcaaccgta tctaaaagta ttgcagcatc
    38281 accgttttgg ttgattagca attattttgt cattactatt gctgagttat gcttaagccc
    38341 aatgggctta tcgtttgtct ctaaaatttc accaccaaaa tataaaggtt ctatgcaagc
    38401 cggatggtat gttgccatgt ctataggcaa tttgcttgcc gggctacttg gggtttatta
    38461 ctatcgctgg ccattgtggg tgttttttgc catgcttgcc atactcagta ttgtctcagg
    38521 gttattaatg atgtctttgc taaggataat aaagcaaact gaagggaagt gaaaacaaaa
    38581 aaaatttatc atgcgtaaaa atatgtatta cttcattttg cccaaccttt tgcagataca
    38641 aaacaaataa ctatatttga aaattgttgt aaggttgtta aaagcgattg tttaatagct
    38701 aatttatcta tcgtttgaag cattaatcag aatgagaaac aaagcatata ttatactttt
    38761 tttattagtt attgcaccaa taaatctttt ggcaagaact tataatattg atagtctaaa
    38821 aatagaatat aaaaatgcca cagaagatac ctccaaaatc aaagttcttt cgctcattgc
    38881 agttagttac ctggataaac aagattatga aaaagtgata gaaaattgcc aggcaataat
    38941 tgaaattgca aatgggaaaa acgacaggat tgaaataatc caagcatatt attggcttgg
    39001 gatatcttat tatagaaaag gtaacgtcaa tgaagcactt tcctattatc taaaagcgct
    39061 tgccatagca aaagttataa aaaacaatta taaatctgac attttaaatg acatagctgt
    39121 tttatataaa ggcagaggag aatatttaaa agctattgaa tattttcaac aaaatcttga
    39181 atatgccgaa caggtaaaag atagcctgat gattggcaag accttaaata acatggccaa
    39241 tgtatatttt tcttatggca tttaccaaaa gtcaattgaa tattatgaaa aaagtatccc
    39301 tatttttcat aacattggca aagagatgta tgtagcttat ctttatataa atctcggttt
    39361 gagctataag aaagttaatc aaagaactaa agccttagaa tcatttaata agagcaaaga
    39421 aatattttct tctctaaaat ttaaggctgg catagcccag gtaaatggag agctggggac
    39481 ttattatgca gaacaaggca actataacat ggccttaagg tttcatgaac aatctcttgt
    39541 tgtttatata gcattaggtg aaaaaatgct tattgcccaa acatatcaag caattggcga
    39601 agtttatcaa aaatcgggag cagcctctac agcattaact tattatgaaa aaagtttggc
    39661 tttgtttaaa gaactcaacg ccaaaaaaga aatgctggac aattatcaga atattgccag
    39721 tatttattct gttttgggag actataaaaa agcttacgaa aatttcaaaa catactccaa
    39781 cctcaaagac tctaccctta gcgatgactt cctgaagcaa atgcaggaaa tggaagccaa
    39841 gtacgaatcg gccaagaaag aaaacgagat aaagctgcaa aaggccgaaa tttcaaagaa
    39901 agatgctgaa agtaaacgca aaaacatctt tatgattgct atcggcttga tagcgcttat
    39961 ggtgattggg tttgcagtac ttatattcaa acaatattca gaaaagaaaa aagccaatat
    40021 cctgcttgcc caacaaaatg acgaaataaa aaaacaacgt gaccagatat tccagcaaaa
    40081 aaaggaaata acggatagca tccattatgc aagccgcata caacgtgctg ttttaccagc
    40141 atcaaaacta ctggatgatg ccggaattaa acatttcatt ttgtacaaac cacgcgacat
    40201 tgttagtggc gacttttatt ggattaacca aaaagacaac aagttgatag ttgctgctgc
    40261 cgactgtacc ggacatggag tgccaggcgc attcatgagt atgttgggca tggccttttt
    40321 gaatgaaatt gtaaccaaag ccgaatttaa caatgccgcc gatatcctca accaacttcg
    40381 cgtattgatt gtaaaatcgt tgcaccaaag cggcaagcaa gaagaaacta aagatggcat
    40441 ggacattact ctttgtatga ttgacaaaga aaactggaat ttacaatttg caggggcttt
    40501 taacccactg taccttatac gtaatggcga aattattgaa ggccctgcag accgtatgcc
    40561 tgtgggcttc catgacaaac ttgaagtgcc gtttaccaac acagagctgc ccatacaggc
    40621 tggcgacacc ttgtatgttt tctctgatgg gtatgttgac caatttgggg gcgaaaatgg
    40681 caagaaattc atggccaaac gtttcaaaca acttcttctc gatatacaag acaaacctat
    40741 ggatacccaa cgcgatattc ttgatactaa tattatagac tggcgcggcg aacttgacca
    40801 ggtggacgat atcttggtta ttggaatgag gttgtaatat ttttattgaa ttaagctttt
    40861 aactaatatt tcaaagaatt ctttttaccg caacagagta ttaatatcca tatcgcaaag
    40921 aactgcaagg aaaatattta aacctttgcg gttctctgcg aatacttttg tgtactctgc
    40981 aatttatctt tctctaaaag cctaattcaa tattttatac tgcatattgc agtttttccc
    41041 tcttaattta cttaaagttt ttataaataa ttgcatattt ttgcactcga aatgaatatc
    41101 tatacagatg agtgtaaaaa caaaaaggct tatcgccatt cgcaaaatac ttagtacttc
    41161 gcaaatcggc acccaggaag aattgctgga agagctttcc aataaaggat ttcaggtaac
    41221 acaagctacg ctttcgcgtg atttaaagat gctcaaagtt ggtaaaatgg ccgaagccgg
    41281 caaaggttat gtctattttt tgccctcaga ccatgaaaac aaagctgcca aagctaccga
    41341 ctcaagagat agttttccta ttaatggttt cctatcgtta actttttcaa aaggactggc
    41401 agtaataaaa accaaatcag gctatgccag cagtgtagct tctctgattg acagttccga
    41461 ttgttatgaa attattggta ccatagccgg ggatgatact attctcatca tccctgttga
    41521 agatgcaaca cctcaagaca ttcgtaatgc cctgaaacta atcatgccag atttggagaa
    41581 taaaatttaa aaacaaattt aaagaccggc caagtagcga aaattaacta aatttgcggg
    41641 tttaaagcgt ttaattatgg gctcagtaaa aaatactaaa ataaccatcc ttggcggtgg
    41701 aaacatcggg atgtcgattg ctgcagggtt aatcaaatca ggccaatgga aagcttcaga
    41761 tttgactatc acccgggtag cctctcaatc acttacagat attgtaaata tgggtataaa
    41821 tgccacccat gacaacaaaa aagcattgaa agatgctgat tttgtttttt tagctgttca
    41881 gccacgtcag gctgtcaagg ttcttgaaga tattaaagaa gccctggatc ctaaaaaaca
    41941 taatataata tctgttgtaa ctgctttaag ctttgccgat atcgaaaaag taataggtaa
    42001 aggtttctct cttacgcgca ttatgcccaa tacagccatt tctatatgcg aatcgatgac
    42061 atgcatggcc agcaatgata ctgaatctga aatgttcaaa aaagcacagg ctttagttaa
    42121 ccaccttgga aaaaccctga ttatagaaga aaagatgatg gcagctgcta cagtgcttgc
    42181 ctcatgcgga acagctttta tgatgcgttt tatccgtgct gcggcacagg gcggcatcca
    42241 ggtaggtttt catgccagcg aggcccagta cattgtagcc caggttgcaa aaggggcagc
    42301 ttgtcttttg atggaaaacg gcctgcatcc cgaacaagag attgacaaag ttactacccc
    42361 tatgggcctt accatcaaag gcctgaacga aatggaacac aacggcctca gctcggctgt
    42421 aatcaaaggg atagtacact cctacaacga aatggaaaaa attaaataat cagaccttta
    42481 aaaaatgaag ctttgggata aaggaactcc tgtaaaccaa cgtgtagagc aatttaccgt
    42541 aggccgtgac cgtgaaatgg ataaaatgct tgcaaaagcg gacatcttag gttcgttggc
    42601 ccatatcgcc atgctcaaca gcattggact tattgcagat gatgaattaa agcttttgtc
    42661 agctgctttg aaagatattt acaaaaacca tcttacggat tttaccatag aacctggtgt
    42721 agaagatgtg cattcgcaaa tagagcttat gctaacccgc aagcttggcg atgtaggaaa
    42781 gaaaatccac agcggccgtt cacgcaacga ccaggtgctg gtggatatta agatttttgc
    42841 ccgcgaacaa atcagggaaa tccatgattt gatattaacg ctttttgaaa cgcttgttgc
    42901 acttagcgaa aaacataaaa atgttctaat cccaggttat acgcaccttc aaatagccat
    42961 gccatcgtct tttggcttgt ggtttggagc ttatgccgag agccttacag atgacctgct
    43021 aatgctgcaa acggcgtatc gcattaccaa tcagaacccg ttagggtcgg ctgccgggta
    43081 cggctcatcg tttccgctta accgcaccat gactacaaag ttgttgggtt ttgaaaccct
    43141 caattttaat gttatttatg ctcagatgaa ccgtggaaag actgaaaaga ccataagctt
    43201 tgcactctct tccattgctg ccacgctggc caagatgtct atggatattt gcctgtttat
    43261 gagccaaaat tttagttttg tcagcttccc cgatgagctt accacaggct cgagcatcat
    43321 gccacacaag aaaaaccccg acgtttttga acttatcagg gccaaaggaa accgcataca
    43381 ggcattgcca aacgagataa gcatgatgct tactaacctt acatcgggct atcaccgcga
    43441 ttttcagctg ctaaaagaaa tccttttccc ggccatggaa gacatgaaag ccatgctgga
    43501 cattgccaac atgatgttgc agaatatcag ggtaaaagac gacatcctga aagacgagaa
    43561 atataagtat atcttcagtg tggaagaagt aaaccgcctg gtattgaaag gtgttccttt
    43621 ccgcgatgct tataaacaag ttggagggca gatagctgaa ggaaaattca atccatcatt
    43681 gacagtaaaa catactcatg aagggagtat tggaaacctt tgtaatgaaa atataagaaa
    43741 aaagatgcaa aatatcagcg aaggcttttg ttttgccaat gttgacaaag ctttacagca
    43801 tttgttacaa taaccccaat ttaaacaatt gattatttta tgaaagacag agcaaggtta
    43861 aaggagaaag agaaaattgt tattaaaata ggtacttcgt cattgactca cgataatggc
    43921 aagataaatt ttaaaagctt tgaaaagctt gccagtgtta tcagtcaaat ggtcaatgat
    43981 ggcaaaaaag tactgttggt tacttcaggg gcaattgctg taggggcggg gaaaataggc
    44041 ctgggacaaa aaccatcaaa attgtccgaa aaacaggcac ttgctgctat cggacaagct
    44101 gaacttatta aaatctacga aaaattcttt gccgaccata acaaaatctc agcccaggtg
    44161 cttttgacaa aagatggtgt gctaaacccc atcaggaggc aaaatgccaa aaatacatta
    44221 aatactctgc tcagcatggg cattgtgcct atcatcaacg aaaatgacac cgtgtcaaca
    44281 gatgagattg aatttggcga taacgatacc ctgtctgccc acgtggcagt ggtttcagaa
    44341 gcagatttgc tggttatctt atcagatatt gacgggcttt ttgacggcga tcctcgtaaa
    44401 gatgctaatg caaaaattat ttcgaaagtt ggcaacgttg acgatgttga aaaatatgca
    44461 ggggcttcca attctgagtt tgccaaaggt ggaatgataa caaaaattgc ggctgcacgc
    44521 ttgtgcaata atgccggcat cgacatggtt attgccaaag gcgaagaccc tgccattgta
    44581 ctggatatta ttgccggaaa agatttaggt actctttttg ttgggaaaac agaaataagt
    44641 atcaaaaaac cagaagaatc tgtcatttat aaagaaaaaa gaggttaggt tgttggtagt
    44701 tgctaaatat tagttatgca gctgctaaaa aaaataaaaa tagaaagatt agaaacaaaa
    44761 tttgaagatg acaaaatcaa aaatcgataa aataacaatc catagtaaat gtttaggcaa
    44821 agaaatgaat gtattagtct atttgcctta tgattacgat aaaaaagaaa atttgacgac
    44881 attgtatttt ttacacggaa gaagtggcga tgaaaatatt ttaatagaag caggtattaa
    44941 tattgttgcg gatagattaa tagcaaatca ggaaatagaa tcaatgataa ttgtctgtcc
    45001 aaatatagag aatagcagag ggctaaattc ttcctttaat tgtaaagagg ttagagatcc
    45061 gttgggtaga ataattaaca taggaatgta tgaagactac ctaataagag aggttattcc
    45121 tgaaattgat aatagatata ataccaatag aaatcgaaat ggcaggtata ttggaggagc
    45181 ttcggcagga ggatatgctg cacttcataa tgcctttcgt cattatgatt tattttcgaa
    45241 ggttggaggg catatgcctg caatagagct taagcttgaa gatgaagaca aagcctattt
    45301 tcaaaatcaa gacatttggg aaaaatacga tccgattaca attgcacaac aaatgaaatc
    45361 ctgtgattta aaggtttatc ttgatgctgg cgataaagat gaaggtcgtt tttatgaagg
    45421 atgtctaatt ttaaataaaa tcctaaatga gaaaggtatt gattcacaaa atcatgtttt
    45481 tgaaggatgc catagtttag agtatataaa atctaatttt gaaaagtata tgttttttta
    45541 cggaaaaaaa taaagccgat cgcataacac gcattatagt taattgctgg tgaagtattt
    45601 gagcggtaca actcgtatca aacctattgc aaactggcag gacagcgttt cgaaagcgga
    45661 aacaaaaaca tactcccaaa acattgatag tcaattaaaa aattaaatta ttgaaatgag
    45721 ccagattata gatatagcaa agcaagcaaa aaaagcgtct attccaatgt cagcattatc
    45781 tgctaaaaca aagaacaacg cattaaaagc catagccaat gcattagtca aagctgaagc
    45841 acaaattatt gaggccaata caaaggattt gcaaaaagca gaacaggaaa agcttgctgc
    45901 tcctttgctt aaacgtttga aattcgataa aaacaagata aaagaggttg ttgatggcat
    45961 cgaaagcctg atacgccttg ctgaacctgt tggccaaaca ctctcggcca ccgaattgga
    46021 caatggtctt gagctttaca aagtaagctg tcctataggg gttataggag ttatttttga
    46081 atctcgccct gatgccttgg tccagatatc atcactttgc cttaaaagtg ggaatgccgt
    46141 tttacttaag gggggcagcg aagctttgca tacaaaccgc attttaacag agataattac
    46201 caaagcatct gcagatgcag gcattcccca ggggtggctc catcttcttg agacccgctc
    46261 ggatgtaaac gaaatgctca aaatggatga ttatatcgac cttatcatcc ctcgcggttc
    46321 taacgagttt gtgaaataca tcatggacaa ttctaacatc gctgtgttag ggcatgctga
    46381 tggtatttgc catgtatatg ttgacaaaga ggctgacata gatatggcag tgtctgttgc
    46441 tgcagattct aagactcaat atgttgcagt ttgcaatgca gccgagactt tactggtgca
    46501 ttcgtctatt gccccaaaat ttctacctgc tttaaaagag aagatggacg ctttgcatgt
    46561 agagttacgt ggttgcgaaa aaacattaaa atatatttca gtaaaacctg ccagcgaaca
    46621 agattgggct acggaatatt tagattatat atggtctgta aaagtagttg attctatgga
    46681 cgaggctatt gagcatataa acaaatatgg ctcggggcat accgagtcta tcattaccaa
    46741 agacaacgtt gcagccttaa aattcatgga tttgtgcgac agtggcaatg tgttctggaa
    46801 cgccagcaca cgctttagtg atggtttccg ctatggatta ggtgctgaag ttggtatcag
    46861 tactaataaa attcatgccc gtggccctgt aggcctcgaa gggttgctta tttacaaatg
    46921 gaaactcatt ggcaacggcc agattgtggc tgattacgct tctgggaaaa aagtttttac
    46981 ccataaaaaa ctagataaaa agtttaaccc aagttaaaca taaaaaatgc ctaattgggg
    47041 ttaaaacagc tttgctttta tttcagcctt ttatggttga aaaaattatt tattaaactt
    47101 tatcttcgct tcaagacaaa aatagctatg gctgataaca ttcaaataga agacagttgg
    47161 aaggaattgc tggctgatga atttgagtca ggctattttg ctgcgctaaa aacatttttg
    47221 attgaagaaa agcagaaata tgaagtgttt cccccgggaa gcctaatttt cagtgctttt
    47281 aacctgacac cggttgacaa agtcaaagtg gtgatagtgg ggcaagaccc ttatcatggg
    47341 aaggggcaag cccatgggct ttgtttttct gttcaacgtg gcataaaacc gccgccatcg
    47401 ttggtcaata tttttaaaga actacaggac gatattggca ttgctattcc atctcatgga
    47461 tgccttgata gttgggcaaa acaaggcgtg ctattactaa atgcaacgtt gacggtacgt
    47521 gccaatacgc ctcgttcgca ttttggcaaa ggttgggaaa aatttacaga tgccgctata
    47581 aaattactat ctgataagcg aaacaacctt gtattctttc tctggggcaa tgatgccaaa
    47641 gcaaaggaaa cattgattga tagcaaaaag catttaatcc tgaaagctgc ccatccttcg
    47701 ccattttcgg cttacaatgg ctttttgggt tgcaagcatt tttcgaaagc caatcaatat
    47761 ctcgtccagc atggttatga gcctgtagat tggaatttga agtaaattgt tttttgatga
    47821 cgacctatat ttctgtttct tgtttttttt gtttccagaa ataaaagagc aatattccgg
    47881 tcaaaaatat tgaggtgaac aatacaggta agttcaaagc actttctact cctgccttgt
    47941 cgatgtcaat gcttgtatag tggttgaaat tcaggtaaaa gaaaacaaca cctaacgcat
    48001 tgttgagaaa gtgggataat acaggtagcc atatactccc actccatagc agcagatatc
    48061 caaacattac accaagtagg aatcgtggga taaatccata gaattggaaa tggatactac
    48121 tgaaaactgc tgctgcaatg ataattcccc aatgtgcatt tttggtccac tcagtaaata
    48181 tgcgttgaaa caagcctctg aaaacaaatt cttcgccaac agcagggatt aaagccatta
    48241 taaacaagtt gagcagcaaa ccgcttattg tactagtttt taagaaggcc tcggtaagtg
    48301 attgggcgtt tttttctgca tctttcatcc agctttctat gcccgacaac cacgacggga
    48361 gtttcatcaa tgagttgatg tgttcggtaa aattgataaa gggtacagca catatcaaga
    48421 taagaatggc aagtaatgca ctctttgcag taggcctttc ttttaaatac aaataattta
    48481 atggcctttg gccaaagaaa aatggcaaag ccaatggggg caatacaaac aaaaatatcg
    48541 actgaaaagt ttgcaggtat ttcaaaatat taataccagt agtcgaatgt atgtcggctc
    48601 cagctgtcag tgttttcagt atgtcatttc cccaaacagg gatagcaaaa agcacactga
    48661 gaaacatccc tatcagcaga aatgttgcca taaatgcaat gcttaacatt atcttaagcc
    48721 cgggtgtagc gttttcgaac cagttgtatc tcatttttaa tgtcaattat tgttgcagat
    48781 tgatttcaaa gcttgtttta tatagacttt atcctgacgt tgtttcttga aactataaag
    48841 cctaactttg cagctacaaa agtaataatt tattggagag ctgaaggttg aaaattgcag
    48901 acattgagtt gggtgagcgg cctgtattcc tggccccgat ggaggaagtg acggatgttt
    48961 ctttcaggcg tttttgtaaa gaatttggtg cagacatgat gtacaccgag tttatttcgt
    49021 ctgacgggct aatccgcaat gcaggtaagt cattaaagaa gctggagata ttcgattatg
    49081 agcgtcccat aggcatacag atatacggcc atctgaaaga tgcaatggta gaggcagcta
    49141 ttattgctga acgtgcaaac cctgatgtga tagatattaa ctttgggtgc ccgatgaaaa
    49201 agattgtccg tcgtggcgct ggatcaggca tgatgaacaa tgtgccgttg atggtagaaa
    49261 ttaccgatgc catcgtcaag gctgtaaaaa agccagttac cgtgaaaacc cgccttgggt
    49321 atgacgagga aaacaaaaac attgtcgata tagccgaaag gcttcaggat gttggcatca
    49381 aagctatcac catccatggc cgtacccgta gccagcttta taccggaaag gcagactgga
    49441 ctttgattgg tgcagtaaaa aataatccac gcatgacaat tcctgttatt ggcaatggag
    49501 atatacgttc tggcttcgat gcttatgagg cttttgaaaa atatggggtg gatggcatta
    49561 tgattggacg ggccactgtt gggtgtccct ggatttttga ggaaataaag cattttctta
    49621 aacataaaag tccacttcct cccaaaaatg tggctgaagt atcaactctt gtaaagaaac
    49681 accttttgct ttctgttgaa ttgaaaggag aacgtggcgg aatactcgaa atgcgacggc
    49741 attttgcgca ttactaccgt gggttgccca attttaggga aacccgtatt aaactcctca
    49801 cctctttgaa tgttgatgaa atattgcaaa ttatcgatag tattgatgag cggtatggag
    49861 agcttttcat gcctcctgtt gacgaaaaac attagttgtt tcattttttt tatctccgca
    49921 tttttttgtt acaaattttc tggactaatt ttatatggat acgtatatat gaagaaaaac
    49981 tttatgcgaa cgtttcattc aaaaatcttt attttctgcg tttttgtttt gacaggtatt
    50041 tcactggctt attctcagca ggctaatgat ggtgtttcga gcaaaggacc cgccaattat
    50101 gatggtacaa aaacaatcaa tgcacaaacc aatgatactg ctaaagttgc tttaaaacag
    50161 gaaactattg acaatgcccc aaaagctgat gttgctccag ccaatacagt catttcggag
    50221 caaacaacct ctgaacgtta tgatactatt gtaaaaaaaa ctggaggaaa gatgaaggtc
    50281 aaaattaccg ggaaaaacgt gcatgaggta acctttttta tgcctggaga gaagaaagaa
    50341 aaaaggctaa gtacgggata tattaaggag cttcgttatg ctgatggacg cattgacctc
    50401 attgacaata cccctgaaaa aatcacagcc aaagatccca ttcttttaat tcctgaaagt
    50461 gaatggaaaa aagtggaagt ggccaaaagt gaaattgagg tgaaagattt ggttgaaaaa
    50521 gaggttattg aagctgaggc cgaagggaaa aaatatacca ctacagatag ttttttggaa
    50581 aaaaatgcaa ttgtcatttt acgaaaaaag gcggtaaacc ttaatgccac taaaatattg
    50641 gttattgata agattgtaac ccgcgagtat ggcgaatttc cgaagatgaa agtgattgcc
    50701 aaagcttatg ccaacgaatg atttggtttt tactttcaat aaatctactt ttgtttttta
    50761 tttctaaaaa agaatggaac gaatagcatt gtttcccggg tcattcgacc catttaccat
    50821 tggtcatgag tcaatcgtaa aacgcgggtt gtctttattt gataaaatca tcattgccat
    50881 agggagcaat agcgataagg ctggtttttt ccctgtcgaa aaacgtatcc aatggattaa
    50941 gcaggttttt gagtacgaag aaaaggtcat ggttgcccaa tataccggcc ttactgtaga
    51001 attttgcaaa gaagtcgggg ctcagtttat tattcgtgga ttgcgcactt ctgctgactt
    51061 tgaatatgag agatctattg ctcaggtgaa taaagctatg gctcctgaaa ttgagtctgt
    51121 ttttatcctt actgcgcccg aacatgcttc ggttacatct tctatagtcc gcgatattat
    51181 caggcatggc ggagatgctt ctaagtttct ccccaatagc caagaattga aaaatttgcg
    51241 aatacactga ttttaatttt ccccaaacta tgatatacaa aaaagaccaa gccgctgaat
    51301 tcgagaaaca gggcgtaaaa atgcgtgttt ataactcgaa agaacaatgt cctgaagctt
    51361 ctgtcgttta ccaggaaacg caaaccggtc accacgagga gttttatcac tccaaaagta
    51421 attttatatt ttatatcatt gaaggagctg gaacctggtt cattgaggat attccttatg
    51481 atgttgaagc tggagatgtt gttattgtgc ctccaggtaa aagattttat tataaaggca
    51541 agcttaaaca ggtttgcatt acatcgcctt catgggagcc tcaatatgag catcatgtaa
    51601 gggatgtaga gttttaatcc taaagtttta ttgtttcatt gcccggtcca tttcgcggga
    51661 agcatcttta cgtttcagat cttcgcgttt atcgaattgc tttttaccac gggcaagggc
    51721 aatatcgagc ttagcaagtc cacgctcgtt tatccacagg ctgatgcaaa caatggtcaa
    51781 tcctttgtcc tgcgactttt tatacagctt gtcaagctct tttttgttta gcaggagctt
    51841 gcgctcgcgg cgtggttcgt ggttgttgca ggtaccccag tcgtattcgg caacatgcat
    51901 attttttacg tacaattcgc cattgacaaa gtaacaataa gcatcgctca ggttagcttt
    51961 tccggcacgt atagatttta tttcggtgcc aacaagcttt atccctgcgg tataacgctc
    52021 caaaaattcg tattcaaacg aagctttttt gttgcggata tctatttttt gttgttctgt
    52081 tttcattttt atagcttggc tgcaaaggta tgcaaaaaag cttttacgtc aaattcggat
    52141 tattgctgca aggcatggct gtaatgcaaa ggctttaata taaaactaaa cgaccagtgt
    52201 attgccaaat aagtaataac aatacatgca aaggccagca acaagatttt cattatcgtc
    52261 aaagggtcag caatggcagc catcttagca cccctgtaaa atgggtacat gccataaatg
    52321 ccaaggaaca taaaaaacga gcccagcgtg gggtaattgg ctaaaatgcc agccaatgcc
    52381 gttgcaaccc aaaagaatgc aagtgaatac agcatgagga taaacagctt atcatagtca
    52441 atctgggcaa tcttccggat agagatttcg tacaccaaca acgacgatac atacaacgaa
    52501 aaaaagcttg ccgcaactac ttctatagct ttgaacagac aatagacaaa tgaataattg
    52561 cccacagtaa tagcactaaa tgtatatccg gccaggatag ctacagcaag tacgcccata
    52621 gctggtaaaa cgtagttttg catgtgccag cgcatttcct gcggttctac aaatatttct
    52681 gcccatccct cgtcagggtg gagaataatc ttcttcgtct tttcccaaag ttgtttatac
    52741 ttttgcatcg ttttgatttt gttgggtaaa aatagtttta tttgtaaagt ttttgaaaga
    52801 atattacaat tttaaaatgt tatcatccaa atacaacctg cttactattc ttgggcctac
    52861 tgcttgcggc aaaacccaac tcgccgttgc tgcggctgct aaattaggct cgtgtgtcat
    52921 aagcgccgat tccaggcagg tttataggca aatggacata ggaacaggca aagatttgtc
    52981 tgagtattct tttgaaggct cgaaagtgcc tcattacctg atagacattt gtcagcccgg
    53041 tgagaagtac aatgtttatg agtttcagaa ggactttttc aatgtttatg agaaagttta
    53101 cgctgaaaat gctatccctg ttttatgcgg aggctccggg ctatacatcg aggcagttct
    53161 gagcaattac cagcttttat cagttccggt caatgaagat cttcgtaata gtctgaaaaa
    53221 taaatctttg gaagagcttg caacgattct tgcttcatat aaattgatgc acaacaaaag
    53281 cgatgtagat acggtaaaac gtgctatccg agctattgag attgcagaat accagaaaaa
//
LOCUS       DAOSPF010000016        52601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_678_length_52601_cov_6.906977, whole genome shotgun sequence.
ACCESSION   DAOSPF010000016 DAOSPF010000000
VERSION     DAOSPF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_52601_cov_6.906977"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PK252_04910"
     CDS             <1..537
                     /locus_tag="PK252_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT14083.1"
                     /translation="YLMTILMQNPNFLILDEPTNDLDIVTLNILEEYLQQFKGCVLIV
                     SHDRYFMDKLVDHVFVFEGNGVIRDFPGNYSDYREYKLANEQIEEPKVKKDAPQPTMP
                     KATSRPKKLSYQQQREFDALTVEIEKLESEKKELEIALASGALNNSEVIEKSNRFSEV
                     CQQIDAKTERWMELSELA"
     gene            681..3272
                     /gene="clpB"
                     /locus_tag="PK252_04915"
     CDS             681..3272
                     /gene="clpB"
                     /locus_tag="PK252_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOT14084.1"
                     /translation="MNLNQFTIKAQEVVQDATLIAQNKNNQAVDTAHLLKSMLDKSDG
                     IAVHLLKKSGVNIQMLAQVVDKIIDSYAKVSGADQYLSSDAIKALQKALDHSKKLGDE
                     FVSVESILYGLLTITDRVAQVLKDAGLDEKTLHTAIQEMRKGAKVTSSNADDTFDALN
                     KYAINLNDRAKKGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIVSGDVPENLKTKLIFSLDMGALIAGASYKGQFEERLKSVINEVVSSNGDIVLFID
                     EIHTLVGAGKSEGAMDAANILKPALARGDLRAIGATTLNEYQKYFEKDKALERRFQVI
                     VIDEPDKLSAVSILRGLKERYENHHKVRIKDEAIIAAVELSQRYITDRFLPDKAIDLI
                     DEAASKLRLEMNSVPQEIDDIERRIKQLEIEREAIKREGDNEKIALLSKDIADLDEER
                     KQMRAKWQAEKDIIDAIQKNKQAIESFKFEADQAERQGDYGKVAELRYGKIKEAEQAI
                     AQLQESLVKQQADWALIKEEVSAEDIAEIVARWTGIPVNKMLQTEKDKLLHLEDELHK
                     RVVGQHEAIQAVADAVRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDE
                     NMMTRIDMSEYQERHTVSRLIGAPPGYVGYDEGGQLTEAVRHKPYSVVLLDEIEKAHP
                     DVFNILLQVLDEGRLTDNKGRVVNFKNTIIIMTSNIGSHIIQDAIDELNRSNRQMVLD
                     RLSIDVMNLLKQTIRPEFLNRIDDLIMFAPLTEADIAEIVKLQFAKVAEHMEGTGIEL
                     ELTEPAIEWLSKEGYDPHYGARPVKRLLQKYLLNELSKKLIDGSFTRGDKILVDSFGE
                     DLVFRKG"
     gene            complement(3453..4433)
                     /locus_tag="PK252_04920"
     CDS             complement(3453..4433)
                     /locus_tag="PK252_04920"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HOT14085.1"
                     /translation="MKKLTNEYQNIIKCPLQLTGTFILLIFRLYIAKKQCKKILGLSG
                     KIHSKGDKRRLGYAFKVVGIVGYFIECITNKPLSQNSKKNLIISGALTPIFDDYFDSI
                     NTSVESINLKIKVKDLTNSLRFDEASRSFFSILQEKTSHTEFAGHLESAIHWQIESTK
                     QKTTKDTLILEEITINKGGSAFTFFASCTDYNLKEKQINAVYNFGAFIQLVDDIFDIY
                     QDEKEGINTLASINIDNLKEISRILTEFIAKSFDNITITNNKNKLARLYALYAFPAYY
                     YLHYLSEMSTKKDFWDASGKLNEKYSWKGWTMPHIMKFIWYMFSKKNFYQ"
     gene            complement(4440..4781)
                     /locus_tag="PK252_04925"
     CDS             complement(4440..4781)
                     /locus_tag="PK252_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT14086.1"
                     /translation="MIKTEKINNVIVVSFNNITRLNALVAEPVKEEIKSLFNKPNTKM
                     ILNLEGVQFIDSSGFGVFLSVMKVANNNYGAFKICNVGNEVMELFKLLQLHNVFEIYP
                     TVDDCLKHFNN"
     gene            complement(4799..5140)
                     /locus_tag="PK252_04930"
     CDS             complement(4799..5140)
                     /locus_tag="PK252_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOT14087.1"
                     /translation="MQTDLTYMKSMFGDNKKVLKEMIDIFAEQVVEISEQMDHAFENK
                     DYVTLGKLAHKAKASVSMMGMKNMAAMLKELELNASEGKYTETFEATVTQFKNSCSIA
                     VEELYNFLEKE"
     gene            5360..6424
                     /locus_tag="PK252_04935"
     CDS             5360..6424
                     /locus_tag="PK252_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14088.1"
                     /translation="MNLLKKIGLFFAFIVIGWLSINGQALSGNDIDKMQRNKKATAAY
                     NKGVDALKTANYSVALQFFNEAVQLNPQSALALLQRGKVKFQMKNKAGAYADFNQAVL
                     LDNKLGECYYALGYLYYSDDSLQKAGELFLKATQNFYEQPEVLYYKGVVNYKLKKYQE
                     AIDDFSKAVALKPNFTLAIFERGIVRLSKEEYQGALYDFRQALQSDSTFLAALNNMAV
                     AKIRSGDFQGAIADLDALIKKQSKNYLAFYNRGCAKFKMKNYDAANEDFKQSQLLNAD
                     YPLVYVGLGNVCAKNGDNNKAIEQYNAAIKLQSDNASAYLNRGLSKELTGDLDGSCVD
                     WQKAAELGNASAELYNNECK"
     gene            6436..8508
                     /locus_tag="PK252_04940"
     CDS             6436..8508
                     /locus_tag="PK252_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT14089.1"
                     /translation="MTISSGFKKICLAIVIASVNVCFTFTQENVKIKKSQFFTVETKF
                     KEAWKAVKSGDKYYKKGIAEYRRAREQYLKAYTYNDKNAALNYKIGVCYLYTDDKPEA
                     IKYLQKAFQIDNNLVWDIHFQLARAYHMNYDFDNAIKEYKLFLASTPKRKLKTITVNI
                     DRCMQECENGKDLITKPVRVVVQNLGKNINSEYDDYASVFDSKEETMYFTTRRLTRKN
                     NKLNAADNKYGENIYISKRRGRDWSLAQPFDQFNTRKSEGMLAVSPDNKKLYFYQSKK
                     NGTIGYSEQEDLEWSRIKAVPKINSRKGRETSVAFTPDGKMMFFVSDKDYKGSIGGKD
                     IYYCVRDSKGDYSKPVNMGATVNTPYNEDAVWVSHDGKTLYFSSKGHESMGGYDIFST
                     SLVNIPDWSKPENIGYPINTPDDDLYFSMSSNGKFGYLSANREKSNGGRDIYRVIYIG
                     AEKEFDMPAGESPAGYAIYPTPGLLDRLPQYFTVDTSVVIMGKVYDADSKKGIMSKIQ
                     FIDSQKSQVVAVVISDSIGNYSAKLPEKKSFGVEINAKGYLFYLDMVNLKDSVKDVVR
                     KDFALKELSIGAKVVLKNIFFETGKATLKEESFQQLEAVIAFMKENPSLKLEISGHTD
                     NVGAMAANIKLSQARAKAVVTYMVTNGIEQERLTFEGYGPKQPIAPNNTKEGKAKNRR
                     VEFKITGK"
     gene            8589..9476
                     /locus_tag="PK252_04945"
     CDS             8589..9476
                     /locus_tag="PK252_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14090.1"
                     /translation="MNKTIYIAFLVFLLVTGCNNHPSENVNKEVAEFEADSVDVIKEY
                     DNAVFCIPSPQFSNLYFKKKGIYPDRSLTLDLNAVENFTTSNQKALNLGVIAVDLAYL
                     NLYTLSEKNEEYLSAVNYLVNGLGLNTVITKEVFENIRRLVNDQDSLAIFLSKIFENA
                     DIFLKDNARQQSAALIVAGGWIESFYLLSKAYEKKPDKELLTFMYQQKFVLENLIRFL
                     SPYYNKSDDVSEIVDDLIEIAYDFDVVDIKYSYKNKAVQTVGQGVIHILNSCKILGNE
                     KTLNNILIEIEELRNKITS"
     gene            9566..9898
                     /locus_tag="PK252_04950"
     CDS             9566..9898
                     /locus_tag="PK252_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14091.1"
                     /translation="MLKDYILDGQQHVLKIKEGLKGKAYFTFFEGFVYRVVVCSNVVK
                     NYKISVFDIENNLIYSKNCDNYSSLLDLKFDANIAGYLLIEVGDKQNINTFTFDLTIG
                     IKEDKAKK"
     gene            9895..12975
                     /locus_tag="PK252_04955"
     CDS             9895..12975
                     /locus_tag="PK252_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT14092.1"
                     /translation="MSEQILKALMQLFAIIARPESNSTDRRTVVQSFLLRLLNEELVQ
                     EYLKIFDGFYFTQQDKTRDTEKHRSIISASSVKVLKIANKINEELTQEQKLIVLFQLF
                     DFVRIDSVEVSQQEIEFIDTIADSFHISLEEYNEIKEFVIHDFSNIPNNERILIVDSE
                     SNFFHPYVKHLEVEAFTGQMRILFISSANMYFLRYIGQNELYLNGQIILEDRAYVFNY
                     GSSIRNPQIKPLYYSDIVARYNEERLKSRILFEAKNIEYKFANGKIGLHNLSFSEESG
                     RLVGIMGASGAGKSTLLYVLNGTNPPTSGEVLINGYNIHTHKEKIEGLIGFVSQDDLL
                     IEELTVFQNLYYNARLCFDNYTEEQLNDIVNKTLVNLGLYEIRHIQVGSPLNKKISGG
                     QRKRLNIALELIREPAVLFLDEPTSGLSSRDSENILDLLKELTLKGKLVFVVIHQPSS
                     DIFKMFDKLLILDTGGYLIYNGDPIESIMYFKSRIHHANWNESECHTCGNVNPEQIFN
                     IVESRVLNEYGQETLARKISPKQWATFFKESFHNGDKEKGLNNDLPPINFKIPGKLRQ
                     FLIFMTRDILSKLSNTQYLVVTFLEAPLLAFILAFIIRYYNVDISNQLGYTFFKNSNV
                     PVYIFMSVIVAIFMGLTNSAEEIIKDRKILKREAFLNLSWGSYLFSKAAVLTIISAIQ
                     ALTFVLVGNSIIEIHGMFFQYWIVLFSAWVSSNIMGLVISDSFKTVVTIYMLIPFLVI
                     PQIILSGIIVKYEKINPLISSPSSIPFYGEIITARWGYEALAVYQFIENDYNRQFYKY
                     NKAMSIASFKKDYWIINLKNKLEMIKSHYQRPEYKDEVANALVVLKNEIGKELKANPK
                     VVYIHLEELTPEKINDAAIEDALTYLEQLNRYYIKLFNKANSEKDEIVNMAQQTPAMR
                     EQFLELKRRCANESLEEFVTNTNEVSRIIEYKGELIQKLDPIYLDPTSPFIKAHFYAP
                     RKMVFGVYMSTFWVNVIVIWLTTVLLFLVLYFRLLLKLLDKLEALGGYFSKSEE"
     gene            complement(12976..13977)
                     /gene="holA"
                     /locus_tag="PK252_04960"
     CDS             complement(12976..13977)
                     /gene="holA"
                     /locus_tag="PK252_04960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT14093.1"
                     /translation="MKEYDEILNELKKKIYKPVYFFHGEEPYYMDRLTEYIAGNILTD
                     AEKTFNQMVYYGKDTSIDKILDAARRFPMMANNQVVIIKEAQELQHIEKLIPYFEKPQ
                     PSTILVINYKKKLDKRTKLATLASKHVSFETKKIPDYKIVEWINNYLKKSGYNIGPEA
                     SQLLADHIGEDLSRIAHELDKLMLVIPKDSKTITKELIEQNIGISKDYNVFELQKTLG
                     TKNHLKANRIIEYLGANPKANPPYIIISVLFKFFSNLLIYHHLDNKSPQHVASSLSLS
                     HVFFVKEVEVAARNYSVQKIITNISILREYDLKTKGIGSVNTPPYELMRELLFKLMH"
     gene            14044..14514
                     /locus_tag="PK252_04965"
     CDS             14044..14514
                     /locus_tag="PK252_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOT14094.1"
                     /translation="MKFPALNLPVADFTLRKQNGKIYVFDVLRKKYVYLTPEEWVRQS
                     FLHYLLFHLQYPQGLIALETPIKLNDNKMRSDIVVFDKQGKPALLVECKAAQIEICQQ
                     TFDQIIVYNMKLNAPYLIVTNGLEHFCFRQPSLGQKIEFFNNIPAYELISGTLG"
     gene            complement(14495..15034)
                     /locus_tag="PK252_04970"
     CDS             complement(14495..15034)
                     /locus_tag="PK252_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT14095.1"
                     /translation="MPLLENELLLLRAPEPTDIDFLYHWENDTDIWQTGITHSPFSKF
                     TLSKYLETAHLDIIEAKQQRFIIVLKSTKNKAIGTVDIFDYDIFNNRAGIGILIAEKQ
                     DRQKGYAFNTLKLICEYCFSTLDMVQLYCNIDTTNTSSINLFEKIGFEAIGVKKKWTR
                     RNDGYHDELLMQIINPKYR"
     gene            complement(15036..16454)
                     /locus_tag="PK252_04975"
     CDS             complement(15036..16454)
                     /locus_tag="PK252_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14096.1"
                     /translation="MDAMKKVLVSVAFMVVKTLACYGQEEINSLKLLTKADMLMFNGQ
                     NDEAANILKQLLNKDPENKYLNFQIGLTLAGKSKDSYKNFLKAYNVPDNNFGQNPVFI
                     TVGEKSTFTDSTSNSKPFNLSSGFKFDLKKPYNNICISKDFTRIVFIDLTSSENKIYF
                     IQKKGNEWLSPADITSQLGSTGDFYPSSFSADGKRLYFTKFDAFGGTIYVSTFDGKTW
                     QTLKKLNSNINSGYSDTHPYETEDGVYLYFASSRPGGFGGMDIYFSVKVNGEWNKAIN
                     LGAVINSNFDEDYPALVNKDNCLLFSTMSISKGKNKAEICKAYRIDDTQWSEPEALGF
                     PFNTPEDDYSYSPLNLYSLNFLYNNILRKNNSNIKQPNTILFETKVMTNKHPASNYSI
                     FINSLSSKDFTKIAETNNNGIATFSLPEGIYQLSYKGSNIAEKNMHIIVPSILKNDTA
                     FINIEIADSPQKENSPANIWKQ"
     gene            complement(16488..17966)
                     /locus_tag="PK252_04980"
     CDS             complement(16488..17966)
                     /locus_tag="PK252_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14097.1"
                     /translation="MMKVLTFKTCLLATILLIIATNNHAQKKPQKGSTQPQLKEIFED
                     AAFFYESGDYREALYNYLKLHEAMPDNHNINFQIGMCYINIPGEETKSLPYLEKASAN
                     ISAKYKANTLEEKKAPLYALYYLGNAYRINNQLTEALNAYNKFRNSPKFESTYNSQMV
                     DNEIDACSRAKIIQDLPVDIKVTPLPSVINTAGTNNHAITNVEGSILIYVSELKFYNA
                     IFVSYRTDTGWSTPQNINPQIGNDGECYPTCISSDGKELFIVKQQKGNNDLYVSVLNE
                     NNKWSLMKPLNKNINSSKNESAATISSDGKILIFSSDRRGGEGKLDLYLSKKDENGEW
                     GKPVNLGNKVNTPSNETNPILCNDDKILYFSSEGHFNMGGYDVFTTTMTDDNKWDVPI
                     NAGYPVCTTGNDLYYYPFNKGTKAFNCLTKEDGFGKEDIYEIENYTVEKNLLSDKKPQ
                     KILVKDEYTKAIIGGLYVNDEETKKKVLSSLQKLSLTINNSK"
     gene            complement(18184..21426)
                     /locus_tag="PK252_04985"
     CDS             complement(18184..21426)
                     /locus_tag="PK252_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT14098.1"
                     /translation="MGKTVFSKTVLKILFFVIIAFNTVLTAQYRNFKSYSIDAGLPNS
                     NIYAIYNDSRGVLWIGSETGLCNFNGLTFKYYTRKNGLPGNWIRAITEDKYGRIWVGT
                     DNGLSIFDGKNFTNYAKIKDTNTLTIYTFFKDSRDNIWIGTSGQGAYKASLSNNKITF
                     VHYNADSAIEAPHIFGIAEANHKIYLGSFGYGIYVIDSKGVTNISQPVIPSNHILSTW
                     SNNSGQVFFSTYNAGVFQLNSKTDKITSSSQIKQYPPATDDMQVWKVLTDKQNNLWLG
                     TNNAGIGRLYPDGISYMGTTAGLPSDFIYTIYQDNEKNLWYGTLTGGLIQFLGDHFSH
                     FNKDDGLIDNVTSIIQQPDGTYYLGTTNGLYHLAFNKNKPVIKPFDRLGGVKINSMLY
                     DKNGYLWIGTGSHGVFAYRGNSLKIYNSNTGLINNEVVCIFNDSQNRIWVGTIGGISI
                     IKDDKFFSFDLTQYGFSTNEIQCIAEDKNKAVWIATLDGLIKMKNNQMISYDEQEGLF
                     DKKTNTLTSDYNGNIWVGTSGGTLYRINPDTTVKKPFIEVANSSHLSCNSIYSLLFYD
                     PQMLLCGTDKGMVKISLDEHKKAKQFFKYDRSNGFIGEENIQNSIYKDRQNQVWMGTV
                     KCLTKYNPKLEEANTSTPHLQIEKIKLFFKDYYADTTSKKLNYFKIKDSISFAYNQNH
                     ITLQFSGISLRNPSKVEYSYMLEGLETTWSPPRQTNEAVYAGLKPGKYCFKLKARGEN
                     GLWSAQPLTYSFEIRPPFWMRWWFYLICSILGLTIFYTYVTWREKKLREDKAKLEQIV
                     LERTTEVVRQKNEIESQRDEISRQHDIVQTQNKDITDSIMYAERIQKALMTKSVGLNK
                     AFNDYFVLFRPRNIVSGDFFWFGQVGNTTFTIAADCTGHGVPGAFMSMLGMSMLNKIV
                     IERNIISPAQILNELRNDIICSLQQNDSQNLNKDGMDITIVAFDKEKMQIHFAGANNP
                     LLLLKNGQIIQTKGDKMPVAIYFEMKDFQQHTFDVSKGDRYYLMSDGYEDQFGGPNGK
                     KFMSKRLNELILQLANTPMGKQEKLFAEAFEQWKGDIEQVDDVLLMGFEI"
     gene            complement(21459..22919)
                     /locus_tag="PK252_04990"
     CDS             complement(21459..22919)
                     /locus_tag="PK252_04990"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOT14099.1"
                     /translation="MKKYLILLLCLTYGINNAQTGNFDTLMFNMFDKLIAASKVKTTA
                     KVVVLGFQGNGKDYKQYDSYLKTIAAIKMNKGKFKLIDQSAVEQAKNSSGWNLSKKND
                     PQVNEAFSQALFEKSGESFTHILYGEIEDMNDKVKITLNLLKNESFTNVASNTEMIDA
                     CEQTDKLLGKAVTHKKMAEDVEQPKVAQQSATSSQPSDSPKEISTSTTTREYVVKETT
                     TDQKVTYRGSTDPLKGLNVAQEKKTMQVGNYYALIIGIDNYKGEWAKLSNAVNDAKAL
                     EDLLKKKYKFDSFRSLYNENATRENIIKELENLIKSVKENDNVLIYYSGHGDFKQELN
                     KGFWVPIDATTNSTSQCISNNDIQTYLGGIKSKHTLLISDACFSGDIFRGKTMTIPFE
                     ETEKYYTKVYNLASRKAITSGGIEPVMDGGKDGHSVFAYYLLKTLSNNNNNMYDASQL
                     FDAIKIPVVNNSSQSPNFQPIKDTGDEGGQFIFIKK"
     gene            23059..24390
                     /locus_tag="PK252_04995"
     CDS             23059..24390
                     /locus_tag="PK252_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT14100.1"
                     /translation="MESKIAFKDILRISLPIIGGSAVENITAIINTAFLGHVGAVQLG
                     AVALGGIMYLIFVMLGFGLGIGMQIIVARRYGSKQLDKIGEVLYHTFFIMLLLGLAFL
                     LAGVLFGPSLLRMIVKSDEIYFNLHAFLSFRIWGIGFAYLNIVFRGFYTGIMRTKVIA
                     VYSIILASVNVFFDYSLIFGHFGFPQMGIAGAGLSSVIAEFAAMSFFLIFTLFHKEIK
                     AFSITRFAGFKKEVLFNLLKVASPVMVQFLISFGGWFFFFTMVENMGEVPLAASNLLR
                     TFYMIALLPLWGFSSATNTLVSYLIGSGRIGEVKKLVLKIMLLSFSSVLMLVLIINIF
                     SAPYLAIFTNDIGLIRACFPAFYIVGLSSLLLSLTIVFFNVISGAGKTTVSLFIEFSV
                     ILVYISWAFLVSHVWHSSVAMIWLAEAFYGFTMGSLALLYLKFGNWRNAKI"
     gene            complement(24391..26220)
                     /locus_tag="PK252_05000"
     CDS             complement(24391..26220)
                     /locus_tag="PK252_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOT14101.1"
                     /translation="MNKIELLSPAQSLEFGKAAINCGADAVYIGAPKYGARAAAGNSI
                     EDIEKLIQYAHIYHARVYVTLNTLLFDNELEEAQEIANKLWNIGADALIIQDMAWLEM
                     KLPPIPLFASTQTHNNTPEKVSFLEKIGIQRAILARELSLGEITKIRKSTNIELEAFV
                     HGAICVSYSGQCYMSQVLTGRSGNRGECAQPCRWLYDLKTADGRLLEKNKHILSPKDL
                     RLDNKLKSMIDAGVTSFKIEGRLKNIAYVKNITAWYRQQLDKIIEESHQTLTKPSSGK
                     SIINFTPDPERSFNRGFTQYFINGRTENMASLYSPKSLGKEVGHIINTKGNWIQAQLS
                     EKISNGDGLCFINKDGIMEGFRVNIAEGSRLQPNENISIPDGTILYRNHDEEFEKKLE
                     KSIQTRKIEADIKVTETPTGLCIESSDEDNNIVNINFDIEKNIATKAEQAFEGLKNTL
                     KKSGNTPFEVKNVDIQFSQPLFFTSAQSNEMRRQCLELLEKERIKNFKKLEYQIIPNN
                     TPFPLTENSYKLNITNKLSKAFYVRHGIASPAAGAEMSDKSSLADLMTNRYCIKYELG
                     ACPKINPNSLLAKEKTLILESHKNRLEVFFDCKNCQMIIRKAR"
     gene            complement(26277..27215)
                     /locus_tag="PK252_05005"
     CDS             complement(26277..27215)
                     /locus_tag="PK252_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14102.1"
                     /translation="MKISGFTMGKNVTKLYYPIKESILSILPICDEFIVALGNCDEDD
                     KTREEILSINSPKIKIIDTVWDIEKYPEGTELAHQTDIAKSYCTGDWLFYIQADEVVH
                     EEDLPKIKKRCEELLNDNEIEGLLFRYFHFWGDYDHHIDSHGWYKNEIRIIRNDKDIH
                     SWRDAQSFRRIPGFDEKNYRQEENTSKLKVAKVDARIFHYGWVRPLNYMQKKIKDFSE
                     IQSKAELQKKIQLERFDYGVMSKIPVYKGTHPKLMQSWIDKLFWKDELAKVTEMPKDR
                     KLTKHEKRKYRFITFLEKYVFFRPMWEFKNYILLKR"
     gene            complement(27227..29050)
                     /locus_tag="PK252_05010"
     CDS             complement(27227..29050)
                     /locus_tag="PK252_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT14103.1"
                     /translation="MKKILSLAEYIKPYWLKVTLSILCNLLSGVFTISTIMFLQPFFD
                     ILFSTQPVSTVPPGDFNILEYKKHFDYIFAQLITEYGQYKTLLIVAFALAFFSLLRNT
                     FAYLAQHLQAPVRAYMLRDIRNKLFDKILQLPLSFFSNEKKGDLMARMTSDVQEIEVS
                     VISSIEMVFRDPLLILIYLATLLTMSVHLTVFVLIMLPISGLLIGRIGRTLRKQSYKS
                     QNKLGSILALIEETISGLRIIKAFNAEKPINKRFKEMNQDFAQMNKRITRRRSLASPV
                     SEFLGTVVMLIILVYGAVLILNKSIDMQPSGLLAFLVTFYLILEPAKKLSSAYYNIQK
                     GLASAERIDFILKAENKIKEKKDAVSKKKFDSVIEYVNISFKYDKHEVLKNINLRIEK
                     GKTVALVGQSGSGKSTLVDLLPRFYDVEHGEILIDGINIKDLKIKDLRSLMGIVNQEP
                     ILFNDTFYNNIIFGRDEIKEEDVYAAAKVANAHDFITSTNHQYHHNIGDRGGKLSGGQ
                     RQRLSIARAVLNNPPILILDEATSALDTESERLVQDALNNLMKNRTTIVIAHRLSTVV
                     HADEICVLHEGQIVERGKHDELLALNGYYKKLNDMQMFSAE"
     gene            complement(29080..30423)
                     /gene="purB"
                     /locus_tag="PK252_05015"
     CDS             complement(29080..30423)
                     /gene="purB"
                     /locus_tag="PK252_05015"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT14104.1"
                     /translation="MKLTELTAVSPVDGRYRDKISELAPYFSEFGLIKYRVLVEVEYF
                     IALCNLPLPQLKDFNKDKYIALRDIYKNFTIEDALEVKNIEKTTNHDVKAVEYLLRKY
                     FDKLGLEQYKEFIHFGLTSQDINNTATPMLLKEAVIEVYVPLLNQILSKLCEVRDEWD
                     KIPLLAHTHGQPASPTRLGKEIQVFIERVEVQKKQLGQIPWSAKFGGATGNFNAHVVA
                     YPQIDWVKFGNQFVNETLGLSRSQTTTQIEHYDNLAATFDALKRINTILIDLCRDMWT
                     YISMEYFKQKVKKDEVGSSAMPHKVNPIDFENAEGNFGIANAIFEHLSAKLPISRMQR
                     DLTDSTVLRNVGVPLSHTVIALKSLQKGMGKVIVNETAIKNDLEKNWAVVAEAIQTIL
                     RRENYPDPYNALKLLTRGNNSINEASIKEFVTSLEVSDAVKKELLAITPFNYTGI"
     gene            complement(30477..31055)
                     /locus_tag="PK252_05020"
     CDS             complement(30477..31055)
                     /locus_tag="PK252_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOT14105.1"
                     /translation="MLQINDTLISLDIIEKKFVCDILQCKGACCIEGDSGAPLEPEEA
                     NKIKNDIEKIKTYLQKEGAKTIDKEGVYVIDTDGDMVTPLINGAECAFAIFNNGIAQC
                     GIEIAWQNGHSSLRKPISCWLYPVRISKYKSFSAVNYHKWDICKNALIKGNNENIPLY
                     VFLKEPLIQRFDNEWYEQLKIAAEHLNSNSGK"
     gene            31170..31820
                     /locus_tag="PK252_05025"
     CDS             31170..31820
                     /locus_tag="PK252_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14106.1"
                     /translation="MAKTQDKKISVILVDDHQLFRNGLKFILGEIEGLEVVGEASNGH
                     EFLQLLEYVKPDLVIMDISMPGMNGIDACRHALSKDPKLKILVLSMYGDDAYYNTMIE
                     LGVKGFILKDSDNKELQNAVRTILSGQNYFSQELLLKIIRNKNVGPAVKLSKRENEIL
                     SLICQGMSTIQISEMLHISHRTVERHRASLLEKTDSSNSIALVVYAIKNNLVTLNK"
     gene            31968..32348
                     /locus_tag="PK252_05030"
     CDS             31968..32348
                     /locus_tag="PK252_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14107.1"
                     /translation="MKKLNVLLVDDNQHFRQALKYMVEESNKEKINNIYQAGSGSEAL
                     NILQMQHIDLVFMDIDMPDMNGIETTRKATELNRFLTIVALSFHQEMQYIMKMLEAGA
                     RNYIIKEDICTDSIKKVFDTVAVN"
     gene            complement(32434..35490)
                     /locus_tag="PK252_05035"
     CDS             complement(32434..35490)
                     /locus_tag="PK252_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14108.1"
                     /translation="MNRFLLSCVLILTAISISGQKKDDQNPREMFLEAESYLLYEEFS
                     EALPLYLKLQKADPENDNLNFKVGLCYLNIPFEKEKAIGYLQKAVKGISPNYNPNSFK
                     ERKAPLDALYYLGNAYRVNNMFEKALETYDEFKSKSNNDSEIYDINLVNDQIKCIERA
                     NNLMSRPIYFVRNNVGEPINSRFEEKNAVVSGDEKTLVYNVKLQFYDALYYSKKVNGR
                     WTAPINIIPDLGVDGDVYATGLSYGGTELYIYRNDNYDGNLYVTRLVNGKWTPLTRLG
                     DNINTKYWESHASLSADGKTLVFTSNRKPGFGGLDIYTATRNDTNNILWTNIRNVGPN
                     VNSEFNEETPFLSQDGKVLFFSSYGHYNMGGYDIFYSTLLSNGTWSTPLNMGYPLNTP
                     DDDLFFMPVQNGDFAYITRYYPDNFGKTDIYKVELFSDQHPRKFILNGMISLKGHAEN
                     NSKIKISLINKTKKDTVKQFSIDQSNPNFVSEITAGNYQMVVEGDGFKKNIENFTIDK
                     NQKESQVNVTSELKPEDVVKTEISQVTSNKPETIEFNKTFYKVENDSVINIIMPLEAG
                     TSVGIMINNKKESKHENLKLKNDNQIYKYKPIEGKNEIIFNATTPEGIKKTGTIIIYY
                     TPVDTTKAQSAAELAMKKREMKYSARMLPFLLPDSAKECLAELDTEENFTSYVELSDF
                     LKTKQSCSALTPLVDSALKHFDSIAAALTQSYALALSKISDGRLKQLADSSAVKKFAS
                     VNQLSDYLIYNTIDNSNDYQQLISNTARLAWQGNAYYYLQNLKKVTSGNLRATLDSVD
                     LSKQKINTGSQLLGYLYAKKDKNSYKEEDIYSAFFALPNFTNIPLVLLNGMAMNTEEH
                     NKDFFENNKKLAGQLKNIESLGSFLHEKALSGVSNDSIILFALVKANEKEYLKQVVSD
                     LKHFSSRVIKENVSFDELEKNNFSTIGEIVNYLFVNNDSVKNEIIRTFAQIGTKNIYQ
                     MNQFEPHKFKPSFFTATNIALISIFFVLMLGLVIYMIRKKSYNTVA"
     gene            complement(35761..37356)
                     /gene="asnB"
                     /locus_tag="PK252_05040"
     CDS             complement(35761..37356)
                     /gene="asnB"
                     /locus_tag="PK252_05040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOT14109.1"
                     /translation="MCGILAIIGKKDLESAKKLSKRMSHRGPDESGIVQFDNGYILTH
                     ERLSIMDLTTGRQPIKGTNNAYVVHNGEIYNYMHLRNTKLADHQFHTTCDSEVIVHLY
                     EKYGYDFCNELDGDWAFVIVDGDKFMASRDPIGVKPFYYGKDAEGNLYFSSEMKTMSD
                     QCEWFEAFPPGHYYSGDTGLVKYYTPKYHDYRQCTENADLKKIRHFLTEATKKRLMSD
                     VPLGVLLSGGLDSSLISSITKRLMRGTDQKLHSFSVGLNKDVPDMVAARKVADFIGTI
                     HHDVTFTIEEGIAAIEKIIWHLESYDVTTIRASTPMYFLSKYITDMGIKVVLSGEGAD
                     EIFGGYLYFHNAPSAEEFTKETIRRVSLLSTADCLRADKSTMAHGLEVRVPFLDRYFL
                     DAVMNIKGEDKQPDRSTGKPEKYILRAAFDDKNDPYLPAEILWRQKEQFSDGVGYSWI
                     DSLVAHCNAKVSDAEFAEAAKRFPHNTPATKEAFYFRTIFEKYYPSESAAKSVLKWIP
                     KWQKNEDPSGRANEVHEKTIEKV"
     gene            37573..38037
                     /locus_tag="PK252_05045"
     CDS             37573..38037
                     /locus_tag="PK252_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOT14110.1"
                     /translation="MEEILQIDSLDRKILSIIQGNARVPFLEVARECGVSGAAIHQRV
                     QKLIKIGVITGSEFIVDPQKVGYHTCAYIGIFLEKAGLFRDVLDELKDIKEIVQCDYT
                     TGNYAIFVKVYTRDNTHLKNIIADKIQAIRGISRTETFICLEECFSRHIPVL"
     gene            38191..38970
                     /locus_tag="PK252_05050"
     CDS             38191..38970
                     /locus_tag="PK252_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT14111.1"
                     /translation="MARVISIANQKGGVGKTTTAINLAASFAVLERKVLLIDADPQAN
                     ATSGTGFETKSIKTSIYQCIIDEADPSSIILNTEIAGFDLIPSHIDLVGAEIEMLNMP
                     NREKMMKQVIEPLKDKYDYILIDCSPSLGLITVNALTAADSVIIPVQCEYFALEGIGK
                     LLSTVKIIQSRLNPALEIEGFLLTMYDPRLRLSNQVVDEVKKHFQQMVFETIVQRNIK
                     LSESPSFGKPCVLYDAESKGTLNYMNLAREIIQKNESVKIK"
     gene            39071..39964
                     /locus_tag="PK252_05055"
     CDS             39071..39964
                     /locus_tag="PK252_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT14112.1"
                     /translation="MNAKKNALGRGLGALIEEANLPQEIVHAVEADSEIEVSLIDVNP
                     YQPRKDFDEEALQELSDSIKQIGIIQPLTLRKMDNGRFQIIAGERRFRAAKMAGLKKV
                     PAFIRIADDQGMLEMALVENIQRADLNAIEIAISYQRLMEECNLTQETLSERVGKKRA
                     TVANYLRLLNLPAEIQAGLRLDNITMGHARALLAVEDNEVKLDIFHQIINDQLSVRDV
                     EEMVRNINNPQQVQEKITLKAPKAATSTSEDYDELRKQLSKRFNAKVDFKRNAEGDGK
                     IIIKFRNDEELEQILSILDTK"
     gene            40006..40569
                     /locus_tag="PK252_05060"
     CDS             40006..40569
                     /locus_tag="PK252_05060"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOT14113.1"
                     /translation="MCKKIFAAVGVSLFLFHSQRLSGQIKGDIDTFDTTKIVLHSPRK
                     ASFYSAVLPGLGQVYNKKWYKVPFIYAGGAVIIYYLKFNHDKYLEYRSNYIRKVANDP
                     NDPVDYKFRYASKTTIKNNMDYWRRNRDWLIVGLSALYIANILDATVDAYLFEYDISP
                     DLSMKMEPVLLNIGNSPTFGVSLNMRF"
     gene            40582..42123
                     /locus_tag="PK252_05065"
     CDS             40582..42123
                     /locus_tag="PK252_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT14114.1"
                     /translation="MTANLRKVLLIVLVFSCFTNIKARQLDDTTDIDQVNQTKVVFED
                     NLDSLLNSYYLQQSDVENDLAVDTVIENVEYSNIPDSVYAERLARIPSVIKMTYNPIV
                     RKYIEMYLQRKKDRVQVMLGLSEYYFPIFDDIFDYYGLPNELKYMSIIESALNCRARS
                     RTRAIGLWQFMYGTGKLYGLTINSLVDERRDPIKETHAAAKFSKDLFNVFGDWQLVIA
                     AYNCGPGNVKKAIRRSGGKRDFWEIYRFLPRETRGHVPAFIAATYVMNYYKEHNLKAV
                     KSDFPRVTDTIVIHDDLHLMQVSYVLGIPIEQLRDLNPQYIRDIIPAKGTSMAITIPA
                     DFTNKFIDYQDSIFAFKDSVFFNPKELNKNPNYEARYYGKAPKGYVKIRHVVKEGESL
                     GAISAKYKCRVNDIMDWNGLYSSRIREGRILTIYVPQKLSKQYLSENTTAAKENAASG
                     LITSDNTKAQGGQIVYYTVKQGDTLWEIAQRYPGISCNDILRWNGLSRGANIVPGQQL
                     KIKVM"
     gene            42206..43540
                     /locus_tag="PK252_05070"
     CDS             42206..43540
                     /locus_tag="PK252_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT14115.1"
                     /translation="MQKNLEKGKIGLFTTSAFVIANMVGTGVFTSLGFQLNGTENIIA
                     VLLLWFLGGIIALCGSLVYSELGAAMPRSGGEYHYLTKIYHPWVGFLSGWVSVTVGFA
                     APVALAAMALGNYTSRVIPVLAPETIAISVLLVITFIHSFDVKVGGKFQNVFTSFKIL
                     LIVFFVVCGFLLVPSFQSFAPSVKSFSLSELLKPEFFVSLIYVTYAFSGWNASAYIAS
                     EIDQPQKTIPKSLIISTLIVTVLYLLLNLVFLMTTPVGELKGQLEVGYIAANKIFGLV
                     GGNLMAVLISILLISTISSMIFVGPRVTQVMGEDIRIFRFLGEKSKRGTPVYALILQF
                     AISFFMILTSTFESVLTYAGFTLNLFTFLTVMGVFVHRKKFPNAERPYKTLWYPWVPI
                     IYMFVILLTIGYLVYIRPYESLMGFITLFSGSVFYIINQWIDTPKKKVKENQ"
     gene            43551..44684
                     /locus_tag="PK252_05075"
     CDS             43551..44684
                     /locus_tag="PK252_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14116.1"
                     /translation="MKLNKVVIAIMIPMALLAIISCNNANGSGINNAKKHSAESVTLN
                     NIANYIAGLETDTSWEQKRLTKFADWQKYKAESKNAWEKFYKEASKYSAFAQKEIPHK
                     YDTIETLFYPFSGPDFLFANTLFPKVKNIILIGLEKPGSVPKLQNLDEKNLADVLNLY
                     KKAIEDVMQLSFFRTLDMKEELANSNIDGTTPVLMLFLVRSEKRINKVEYINLNENGN
                     LVVVDPEKVKDYIGVKIYYTDKDNEVEKTILYLSTNLADPSLSKDKAFMAFLKNIDNP
                     CITLVKSATYLMHKSYFSIIRNTCLEHSALIVQDDSGIAYKFFAPEKWDLSLYGSYTK
                     PIKLFEEFYEPELTKAFETKKAKPINFRFGYSQKSCILIAERK"
     gene            44736..45596
                     /locus_tag="PK252_05080"
     CDS             44736..45596
                     /locus_tag="PK252_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT14117.1"
                     /translation="MFIFINIEAESISKALPGVDTSYVTSEDSLFKQDTVYVEELQES
                     DFQDYLDTVSMAVDTVFWDNKMINSGRFDSKNMCDTVSFVLRDSTTGKNFYIPFKNYI
                     TSNFGPRRYIFHYGVDIKLFKGDSVHAAMDGVVRVTKYDRRGFGNVIVIRHSSGLESI
                     YGHLSKVLVEPNQKVKAGEVIGLGGNTGRSTGSHLHLEFRYKGEPFDPNYVIDFEKSC
                     LRRDTLVLTRNNFEYLVELRKAKYCTVRKGDNLGKIARRYHTTVAKLCSLNHINRKTI
                     IRAGRKLRYQ"
     gene            45609..46964
                     /locus_tag="PK252_05085"
     CDS             45609..46964
                     /locus_tag="PK252_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT14118.1"
                     /translation="MSQSNNTGAPFLVRWVILIIISVAMMGNYYIYDSISPLADMLKV
                     QLGFSSTAIGTLNAIYSIPNIFMVLIGGIIIDKIGVRKAGLIFTALIMFGAVITCLKG
                     SLIVMATGRLIFGLGAESMIVAITTSIARWFKGKEFSFAFGINLTVARLGSFLALNSP
                     NWAASFYAKGWQFPLFIAAIAGIAAVICMVVFYFADGIAGKRYHLPKEGNQDKIEWKA
                     IFKFSTSFWFISMLCIVFYSAMFPFQTFAVMFFEHSYNLDNGAGGLLSSMLTLAAMIF
                     TPIFGYLSDLVGRRSLFMIIGSLMIIPLYLMLGYHVDLVSALHLSSTWHVSIKILSID
                     AFISPNVLIPMFVMGLAFAMIPALMWPSVALVVDNSKLGTAYGLMTMIQNIGLVGFNL
                     LIGVVNDHFQAGAGNPDGYIPGMWLFSMCGVLGIVFSFLLRKSAKTEKGVTLDLPMRK
                     K"
     gene            complement(46969..47169)
                     /gene="xseB"
                     /locus_tag="PK252_05090"
     CDS             complement(46969..47169)
                     /gene="xseB"
                     /locus_tag="PK252_05090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT14119.1"
                     /translation="MSKKGLTYENAMAEIEMIVEGIENNQFSIDELSEKVKRISELVL
                     FCKEKLRQTEEQVNKIINEIKE"
     gene            47267..48274
                     /locus_tag="PK252_05095"
     CDS             47267..48274
                     /locus_tag="PK252_05095"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT14120.1"
                     /translation="MKVAIVGVSGAVGQEFLKVLEERNFPADELVLFGSARSAGNSYN
                     FMGKSITVKELKHNDDFKGIDIALTSAGASTSKEFATDITKFGAVMIDNSSAFRMDAD
                     VPLVVPEVNPMDCKNRPRNIIANPNCSTIIMVVALKPIQDLSPIKRIHVATYQAASGA
                     GAAAMDELFAQMKQVVNHEKVHVEKFKYQLATNLIPQIDVFTDNDYTKEEMKMHNETR
                     KIMHSDVCVSATTIRVPVLRAHSEAVWVETEKPLEVAAVRAAFEKAEGLVVQDDPKNS
                     IYPMPLMVAGADPVYVGRLRKDIANPNGITFWCVGDQIKKGAALNAVQIAEYMIKNRL
                     L"
     gene            48414..49397
                     /locus_tag="PK252_05100"
     CDS             48414..49397
                     /locus_tag="PK252_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOT14121.1"
                     /translation="MKKLNEIKKGIFFILAMMAMMDISAQNPVGRHGFLKVKGNKIVG
                     EKGEEAQLRGMSLCWTQWFGKHYNYQTVQWLQEDWKCDVVRAAMAVEHDGYLVHPDME
                     QMKIETVINAAIDIGMYVIIDWHDHHAQRNVEAAKRFFGEMARKYALFPNIIYELYNE
                     PLQISWKDSIKPYCEAVIAEIRKHDKRNLVICGTPTWSQDVEVAAADPVKDENVAYAM
                     HFYAATHKQFLRDKTKKALDMGVAVFVSEFGICDASGRDPIDYEETKTWLKFCDENKL
                     SYCNWGIYDKKEAAAALYPGKNTFGGWTFEMLTPSGKFIRAWLRKEALVTK"
     gene            complement(49578..50288)
                     /gene="aat"
                     /locus_tag="PK252_05105"
     CDS             complement(49578..50288)
                     /gene="aat"
                     /locus_tag="PK252_05105"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOT14122.1"
                     /translation="MPVFELCADLIFPHPSYAEEDGFLAYGGDLTSERIILAYKNGIF
                     PWFNPGDPIMWWSPNPRCVLFPEKLKVSKSLLTVLKKKKFTIKCDSAFLEVIVHCAKT
                     ARKKQTGTWITNEIIDAYANLHKMGIAHSIEAYQNDKLVGGLYGLAIGKVFFGESMFY
                     HVSDASKVAFCHLVKYLNVKGYQIIDNQMQTEHLMRFGSELIDRSAFLDIVKKACAEE
                     INTSNWTGFNSFCSNIAW"
     gene            complement(50292..51029)
                     /locus_tag="PK252_05110"
     CDS             complement(50292..51029)
                     /locus_tag="PK252_05110"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT14123.1"
                     /translation="MEHLWPIVSLNTVSSTNNYALDLIKQNQNLAEQVISTRTQSNGK
                     GQAGNTWESESNCNLTFSLIIKPRDLKAENQFAITIASSLGILDFLNENKISSRIKWP
                     NDILHGRKKIAGILIENTIIGEYIQHSVIGIGLNINQTDFKQLSGTAISMKEITGMYF
                     NLDIALHQLLNCLKSRIEQMYKTDFSQFKNIYLEHLYGFQKWLKYSDMNHHFEGKITD
                     VLDTGELVLEDEHGQLSTFMHKEIALV"
     gene            51258..>52601
                     /locus_tag="PK252_05115"
     CDS             51258..>52601
                     /locus_tag="PK252_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT14124.1"
                     /translation="MSKRFRFGLIGLLQITILNIFAQDIEQKQVSVSTEKTSLIQFLN
                     KVSTENQVQFYYKKEWFEGDSIALTISDIQLGRLLDKISDSRAIMWLPISEDAIVFLP
                     KDQVATLLGNMVDMSGSKNINTVEVGKMSEAGKYANVILKGTVKDGKNDEPVIGASLR
                     IANTNIGCMTDANGKYQLRLSPGIYEVEVSSVGYEKNVWKVKLISTGEFNPEIFEKST
                     KIDEITIYAKRADKNVRSNQMSLIDLDSKSIKQLPSMVGGKDIIKSFTMMPGVKSVGE
                     FGSGINVRGGGDDQNLYLIEGAPLLNTSHVLGMMSVLNPDIVSNVALYKGQIPANYGE
                     RVSSVMEIQVRENNIKNFHVKGGIGLYDGRVLVEGPISKKVTYKIGGRSSYSNWLLGV
                     IPDANIQKSAASFYDLNSYISWAGEKDRVSLFAYYSNDYFRYARLQAYNYGNILGS"
BASE COUNT        15621 a         8737 c        10423 g        17820 t
ORIGIN      
        1 tatctgatga ccatcttgat gcagaaccct aatttcctga ttctcgatga acctaccaac
       61 gacctggata ttgttacact gaatattctt gaagaatatc ttcagcaatt caagggttgt
      121 gtgctaatcg tatcgcacga ccgctacttt atggacaagc tggtagatca tgtgtttgtc
      181 tttgaaggga atggcgttat tcgggacttt ccgggcaact attccgatta tcgtgaatat
      241 aaattggcta atgaacagat agaagaacca aaagtcaaga aagatgcacc acagccaact
      301 atgcctaaag ctacatcccg tccaaagaag cttagctatc aacaacagcg tgagtttgat
      361 gctttaactg tcgaaattga aaaactcgaa tcggaaaaga aagaactgga aattgcttta
      421 gcttcaggag ctctcaataa ttctgaagtt atagaaaaaa gcaatcgctt ttctgaagtt
      481 tgtcagcaaa ttgatgctaa aacagagcgt tggatggagc tcagtgagtt ggcctaaggc
      541 tttttcattt tacatcattt aaaagtcgaa aaatttaaat atgacataat ggctatattt
      601 ttcattaact tatgacattt tgacttattt tattatttgg tgcttatttt gtactaactt
      661 agataaacaa aataagtatt atgaacctca atcaatttac aataaaggcg caggaagtag
      721 ttcaggatgc tactcttatt gcgcagaata agaataatca ggccgtagat actgcgcacc
      781 tgcttaaaag tatgttggat aagtctgatg gtattgccgt tcacttgctt aagaaaagtg
      841 gtgtcaatat tcagatgtta gctcaggttg ttgataaaat cattgattcg tatgccaaag
      901 ttagtggagc tgaccagtat ttgtcttcag atgctataaa agcgttgcag aaagcattgg
      961 atcattcaaa gaaattaggt gatgagtttg tttcagtgga gtcaatcctt tatggtttgc
     1021 tcactataac agatagagtt gctcaggtgc ttaaagatgc aggcctcgac gaaaaaacat
     1081 tgcatacagc cattcaggaa atgcgtaaag gcgctaaagt aacatcaagc aatgccgatg
     1141 atacttttga tgcattaaat aaatatgcta taaatcttaa tgacagggct aaaaagggta
     1201 agcttgatcc agtaattggc cgtgatgaag aaattcgtcg tgttttgcaa atcttatcgc
     1261 gccggaccaa aaacaaccct attcttattg gcgaacctgg cgtaggcaaa acagctattg
     1321 ccgaaggcct tgctcaccgt attgttagtg gcgatgtacc tgaaaatttg aaaacaaagc
     1381 tcatattttc attagatatg ggggcattga ttgccggggc aagctataaa ggccaatttg
     1441 aggaacgtct aaagtcagta atcaatgaag tggtttccag caatggggat atagtgctgt
     1501 ttattgatga aattcatacc cttgttgggg caggaaagag tgagggggct atggatgctg
     1561 caaacattct gaaaccggct ttggctcgtg gtgatttgcg cgccattgga gcaactacgc
     1621 taaatgaata tcaaaagtat tttgaaaaag ataaagccct cgaacgcagg tttcaggtga
     1681 ttgtcattga tgaacctgac aagctcagcg ctgtttctat tttgcgtggg ttgaaagaaa
     1741 ggtatgagaa ccatcacaag gtacgtatca aggatgaggc tatcattgct gctgttgagc
     1801 tttcgcaacg gtacattaca gaccgcttct tgccggacaa agctattgac ctcatagatg
     1861 aagctgcatc taagcttcgt ctggagatga actctgtacc gcaagaaatt gatgatattg
     1921 agcgaaggat aaaacagctt gagattgaac gcgaggccat taaacgtgaa ggtgataatg
     1981 aaaaaatagc cttgctttct aaagatattg ccgatttgga cgaagaacgc aaacaaatgc
     2041 gggctaaatg gcaggccgaa aaggatatca ttgatgctat ccagaagaac aagcaggcta
     2101 ttgaatcttt taagtttgaa gctgaccagg ccgaacgtca gggcgattat ggcaaggttg
     2161 ctgagcttcg ttacggtaaa ataaaagaag cagaacaggc tattgctcaa ttacaggaat
     2221 cgctggtaaa gcagcaggca gattgggctt tgatcaaaga agaagtgtca gctgaagata
     2281 tagccgagat tgttgcccgt tggactggca tcccggtaaa taaaatgtta caaaccgaga
     2341 aagataaact gcttcatctc gaggatgagc tgcataaaag ggttgtaggg caacacgaag
     2401 ctattcaggc tgtggccgat gctgtccggc gtagtcgtgc cgggctgcaa gacccgaaaa
     2461 ggccaatagg ctctttcatt ttccttggta caacgggggt tggaaaaact gaattggcga
     2521 aggcattggc tgagtttctc tttgatgatg aaaatatgat gacccgcatt gatatgagcg
     2581 aatatcagga acgtcatacg gtttcgcgcc ttattggtgc gcctccgggc tatgtcgggt
     2641 atgatgaagg gggccaactt actgaggctg ttcgccataa accttattct gttgtacttt
     2701 tggatgagat tgaaaaggcc caccctgatg ttttcaatat tttgcttcag gtgcttgatg
     2761 aaggccgttt gacagacaat aaaggaaggg tggtcaattt caaaaatacc attatcatta
     2821 tgacttctaa tattggttcg catattatcc aggatgcaat tgacgagttg aaccgctcta
     2881 accgccaaat ggttctcgac aggctttcta ttgatgtaat gaatctcttg aagcaaacta
     2941 tcaggccaga gtttcttaac cgtatcgatg accttatcat gtttgcccct ttaacagagg
     3001 ctgatattgc tgaaattgta aaattacagt ttgctaaagt ggcagaacat atggaaggaa
     3061 caggtattga gctcgaactt acagaacctg caatagagtg gctttcaaaa gaagggtacg
     3121 accctcatta tggggcaaga cctgtaaaac gcttgttgca aaagtatttg ctcaacgagc
     3181 tttctaaaaa gcttattgat ggttctttca cacgtggcga taaaatattg gttgatagtt
     3241 ttggcgaaga cttggttttc aggaaaggat aaatttgggt atgtaaacct gttttaaggt
     3301 agaaaatccc ggagggatta aatatttgta gccccggcat tcatgccggg gtaatggtat
     3361 agcatgtatt ttagcgacgg agtaaggtgt aaaagtgaaa agggtatgta tttcccgtcg
     3421 caaaagaatg attttattga aacttgggat ggttattgat aaaaattctt cttagaaaac
     3481 atataccata taaacttcat tatatgtggc attgtccagc ctttccagct atatttctcg
     3541 tttagctttc cggatgcatc ccaaaagtcc ttttttgttg acatctctga aagataatgt
     3601 aagtagtaat atgctggaaa ggcatataag gcatataatc ttgcaagctt gtttttattg
     3661 ttggttatag ttatgttatc aaatgatttg gctataaatt cggtaagaat cctgctaatt
     3721 tcttttaaat tgtcaatgtt tatgcttgct aatgtattta tgccctcttt ttcatcctga
     3781 taaatatcaa aaatatcatc aacgagttga ataaatgccc caaaattata cacagcattt
     3841 atttgctttt ctttaaggtt ataatcagtg caggatgcaa aaaatgtaaa agctgaacct
     3901 cctttattta ttgttatttc ttcaagtatt aaggtgtctt tagttgtttt ttgtttggta
     3961 ctttctattt gccagtgtat agcagattca agatgtcctg caaattctgt atgagatgtt
     4021 ttttcttgca aaatggaaaa aaagcttctg gaagcttcgt caaatcttaa actattcgtc
     4081 aaatctttga cttttatttt taaattaata ctttcaacag aggtgttaat tgaatcgaaa
     4141 taatcatcaa atattggcgt taacgctcct gagataatca ggtttttctt actattttgg
     4201 cttagaggtt tgttagtaat acattctata aaatatccaa ctatccctac aactttaaaa
     4261 gcataaccta agcgtctttt atctcccttt gaatgtattt tccccgataa acctaatatt
     4321 tttttacatt gttttttagc aatataaagc ctgaagataa gtaaaataaa agtgcccgtc
     4381 agttgtaacg ggcactttat tatattttga tattcattgg taagtttttt caatattatt
     4441 taattgttga aatgttttaa gcaatcatcc acggttggat aaatttcaaa tacattgtgc
     4501 aactgtaata gcttaaacag ctccataact tcgttcccta cgttgcaaat tttaaaagcc
     4561 ccgtaattgt tattagctac tttcatgacc gaaagaaata ccccaaatcc tgagctgtct
     4621 ataaattgta ctccttcaag gttgagtatc atcttcgtat ttggtttatt gaacaagctt
     4681 ttgatttctt ctttaacagg ctctgcaacc agggcgttaa gtctcgtgat gttattaaag
     4741 gaaacaacga taacgttgtt gattttttcc gttttaatca tggtattata gttttaaatt
     4801 attccttttc taaaaaatta taaagttctt caacagcaat tgagcaacta tttttaaatt
     4861 gtgtaactgt agcttcgaac gtttctgtat atttgccttc agaggcatta agctcaagct
     4921 ctttaagcat agctgccatg tttttcattc ccatcatcga cacagaagct tttgctttgt
     4981 gcgcaagctt gccaagagta acataatctt tgttttcaaa agcatggtcc atttgttctg
     5041 aaatttcaac cacctgctcg gcaaagatgt caatcatttc tttcaacacc tttttgttgt
     5101 cgccaaacat gcttttcata tatgtcaaat ctgtctgcat tacccttctg attgtaacaa
     5161 atgtacaata ttttttataa ctttaaaaat gaaaaaacct ttagttcgtt catttaatat
     5221 ttcaaagtaa attatttttt ttatatttat caattattga tgatttgttt ttgctggttt
     5281 taaaaagtgt ttactgttat tttcaagtaa aaatgagtca tttatatttt aatttgtagc
     5341 aaattttaat taatataata tgaacctcct gaaaaaaatt ggtctttttt ttgcctttat
     5401 tgtcattggc tggttaagca ttaatggaca ggcattatca ggtaatgata ttgataaaat
     5461 gcaaagaaac aagaaagcta cagctgctta taacaaaggg gttgatgctt taaaaactgc
     5521 taattactcc gtggcgcttc agtttttcaa tgaagctgtg cagctcaacc ctcagtctgc
     5581 attggcattg ttgcaaagag ggaaagtcaa atttcaaatg aaaaacaaag ctggggctta
     5641 tgccgatttc aatcaggcag ttttgttaga caacaaatta ggggagtgtt attatgcatt
     5701 aggttatttg tattattcag atgatagttt gcaaaaagct ggagaattgt ttttaaaggc
     5761 tactcaaaat ttttatgaac agcctgaagt cctttattac aaaggtgtag tcaattataa
     5821 acttaaaaaa taccaggagg ccatagatga tttttctaag gctgttgctt tgaaaccaaa
     5881 ctttactttg gctattttcg agagaggcat tgtacgttta tctaaagaag aatatcaggg
     5941 ggctttgtat gattttcgtc aggcattgca gtctgattct acttttttgg cagcattgaa
     6001 caatatggct gttgctaaga ttcgctctgg cgattttcag ggggctattg ctgatttgga
     6061 tgctttaatt aaaaaacaaa gtaaaaatta tctcgctttt tacaatagag gttgtgcaaa
     6121 attcaaaatg aaaaattatg atgcagccaa cgaagatttc aagcagtcac aactgctaaa
     6181 tgctgattat cctttagttt acgtcggact tggcaatgtt tgtgctaaaa atggcgacaa
     6241 taacaaagct attgaacaat ataatgctgc tataaaactg caatcagaca acgccagtgc
     6301 ttatcttaac agggggcttt ctaaagagct cacaggtgac ctcgacggct catgtgttga
     6361 ttggcaaaaa gcagccgagc ttggaaatgc cagtgctgaa ttatacaata atgaatgtaa
     6421 ataaacttgc ttaatatgac aatatcatca ggttttaaaa agatttgttt agccattgtg
     6481 atcgccagcg tcaatgtatg ctttacattt acccaggaga atgtgaaaat caaaaaatct
     6541 cagtttttta ctgtcgaaac caagtttaaa gaagcctgga aagctgtgaa atctggcgat
     6601 aaatattata aaaaaggaat agctgaatat cggcgggccc gcgagcaata tctgaaagca
     6661 tacacttaca acgataaaaa tgcagctctg aattataaaa ttggggtatg ctatttatac
     6721 acagatgaca aacccgaagc gattaaatat ctgcaaaaag cttttcagat agacaataac
     6781 ctggtttggg acattcattt tcaattggca cgggcttatc atatgaacta cgattttgac
     6841 aatgctataa aagaatacaa gttgtttttg gccagtaccc ccaaacgaaa acttaaaact
     6901 attacggtca acattgacag gtgtatgcaa gagtgcgaga atggaaaaga tctaattact
     6961 aagcccgtaa gagttgtagt gcaaaatctc ggcaaaaata ttaattctga gtatgatgat
     7021 tacgcatctg tttttgacag caaggaagaa accatgtatt ttacaacccg ccgcctgacc
     7081 agaaaaaaca acaaacttaa tgctgctgat aataaatatg gcgaaaatat ttatatttca
     7141 aagcgtagag gccgcgattg gtctctggca cagccttttg accagtttaa tacccgcaaa
     7201 agcgaaggga tgcttgctgt ttcgcctgac aataaaaagc tttactttta tcaaagcaaa
     7261 aaaaatggaa ccataggtta ttctgaacaa gaagatttag aatggtccag aattaaagca
     7321 gttccaaaaa ttaattcgcg caaagggcgc gagacatcag ttgcatttac cccagacggg
     7381 aagatgatgt tttttgttag tgataaagac tacaaaggtt ctattggggg caaggatatt
     7441 tattattgtg tccgagattc aaaaggcgat tattcaaaac ccgtaaatat gggggctact
     7501 gttaatactc cttataacga agacgctgtt tgggtttctc atgatggaaa aacattatat
     7561 ttttcgtcaa aaggtcatga gtctatggga ggttatgata ttttcagtac ctctttagta
     7621 aatataccgg attggtcaaa gcctgaaaat attggatatc caatcaatac tccagacgat
     7681 gatctctatt tttcaatgtc ttcaaatgga aagtttggtt atttgtctgc caatcgtgaa
     7741 aaatctaatg gaggccgcga catttatcgt gttatatata taggcgccga gaaagaattt
     7801 gatatgccag caggagaaag ccctgcaggt tatgccatat atccaacccc aggattatta
     7861 gaccgtttac ctcaatattt tacggtagat acctctgttg ttattatggg taaggtgtat
     7921 gatgcagata gcaaaaaagg gataatgtcc aagatccaat ttattgattc gcaaaaatca
     7981 caggtggtag ctgttgttat aagtgattct atcggtaatt attcggcaaa gctccctgaa
     8041 aagaaaagct ttggggttga aatcaatgcc aaaggctatc ttttttacct tgatatggtt
     8101 aacttgaaag attctgtaaa agatgttgta cgtaaagatt ttgcgctcaa agaattgagt
     8161 attggggcaa aagttgtatt gaaaaatatt ttctttgaaa ctggtaaagc tacattgaaa
     8221 gaagagtcat tccagcaact tgaagcagta atagctttta tgaaagaaaa tccatcattg
     8281 aagcttgaaa tatctggcca tacagacaat gttggagcaa tggctgctaa tattaaactc
     8341 tcacaagccc gggccaaggc ggttgtaacc tatatggtta ccaatggcat tgagcaggaa
     8401 agattgacct ttgagggata tgggcctaaa caacctattg ctcccaacaa tactaaagaa
     8461 ggaaaagcta aaaaccgaag ggtagagttt aaaattactg gtaagtaatt acttgtcgaa
     8521 atggatattt gcctgtttca atattttcat tatttttgga gtgacaatct attttacccc
     8581 aaagcgtaat gaataagaca atttatattg cttttcttgt atttttgctt gttacaggat
     8641 gtaataacca tccttctgaa aatgtaaaca aagaggtagc cgaatttgaa gcagattctg
     8701 ttgatgttat caaagaatat gataacgctg tgttttgtat tccttctcca cagtttagca
     8761 atttgtattt taagaaaaaa ggcatttatc ctgatcgatc attgacctta gatctcaatg
     8821 ctgttgaaaa cttcaccact tctaatcaaa aagccctcaa tcttggggtt atagctgtag
     8881 atttggctta tttaaacctt tatacattat ctgaaaaaaa cgaagaatat cttagtgccg
     8941 tcaattattt agtgaatggg ttgggcctta atactgttat tacaaaagag gtttttgaaa
     9001 atatcaggag actagttaat gatcaggatt ctttggcgat atttttatcc aaaatatttg
     9061 aaaatgccga tatttttctt aaagataatg caagacagca gtcagcagca ttgattgttg
     9121 ccggaggttg gatagaaagt ttttatcttc tttcaaaagc ttatgaaaaa aaaccagata
     9181 aggaattgct gactttcatg tatcaacaaa agtttgtcct tgaaaacctc atccgttttc
     9241 tttcacctta ttacaataag tctgatgatg tgagtgaaat tgtggacgat ttgatagaaa
     9301 tagcttatga ctttgatgtg gtggatataa aatatagcta taaaaataaa gctgtacaaa
     9361 cagtgggcca aggggtaatt catatattga atagttgtaa aattttgggc aatgaaaaga
     9421 cacttaacaa tatattgata gaaatagaag aattgagaaa taaaataacc tcataaaata
     9481 gccaatgttc aaatatcttt ttatattatt cattagttgt tccacatatt tgtgtgccgg
     9541 agattgtttt aaaagcacca gtgatatgct caaagattat attttggatg gacagcaaca
     9601 cgtgttgaag attaaagaag ggttaaaagg aaaggcatat tttacttttt ttgaaggatt
     9661 tgtatataga gttgtggttt gcagcaatgt agtcaagaat tataaaattt ctgtttttga
     9721 cattgaaaat aacctgattt attcaaaaaa ctgtgacaat tattcgtcat tgctggattt
     9781 gaaatttgat gcaaatattg caggttattt acttattgaa gttggagata agcaaaatat
     9841 aaatactttt acatttgatc tgacaattgg aattaaagag gataaagcta aaaaatgagc
     9901 gaacagatac ttaaagcttt gatgcaatta ttcgcaatta ttgcgaggcc cgaaagtaat
     9961 tcaacagaca ggaggacagt tgtacaatca tttttactca ggttattaaa tgaggagctt
    10021 gttcaggaat atttaaaaat ttttgatggt ttttatttta ctcaacaaga taaaacacgc
    10081 gatacagaaa agcatcggag cattatttcg gccagttctg ttaaagtgct caaaatagcc
    10141 aataaaatta acgaagagct tactcaggag caaaagctaa ttgttctttt tcaacttttt
    10201 gactttgtca ggattgatag cgttgaagtt tcgcaacagg agatagaatt tatagatacc
    10261 atagccgact cgtttcatat ttcgttagaa gaatataatg aaatcaaaga atttgtaatt
    10321 catgatttca gcaacatccc caataatgaa agaatattga ttgttgacag cgaatcaaac
    10381 ttttttcatc cctatgtaaa acatttagaa gtagaggcct ttacagggca gatgagaata
    10441 ttgttcattt cttcggctaa tatgtatttt ctgcggtata ttgggcaaaa tgagctttat
    10501 ctcaatggcc agattattct tgaagaccgt gcgtatgtgt ttaactatgg ttcatctata
    10561 cgcaatccac aaataaaacc tttgtattat agtgatattg ttgcccgtta caatgaagaa
    10621 cgactcaaat cgcgtatttt atttgaagcc aaaaatattg aatacaaatt tgccaacgga
    10681 aaaattggtt tgcataattt gagttttagt gaagagtcag gcaggttagt gggtattatg
    10741 ggagccagtg gtgccgggaa gtcaactttg ctttatgtgt tgaatggtac taaccctcca
    10801 acatctggag aagttttaat caatgggtat aacattcata cccataagga aaagatagag
    10861 gggcttattg gttttgtttc gcaagatgat ttattgatag aagagcttac agtatttcaa
    10921 aacttgtatt ataatgcccg tttgtgtttt gataattata ccgaagaaca gctcaacgat
    10981 attgtaaata aaactttggt taacctgggg ttatatgaaa tccggcatat tcaggtaggt
    11041 tctcctttga ataaaaaaat tagcggaggg cagcgtaaaa ggctcaatat agcgcttgaa
    11101 cttattcgcg aaccagcagt attgtttctt gatgagccta cctcagggct ttcgtcgcgc
    11161 gattcggaaa atatcctcga tttgctcaaa gagcttacgc tgaaaggaaa attggtattt
    11221 gtggttatcc accagccttc ctccgacatt tttaaaatgt ttgacaagtt gcttattctg
    11281 gatacgggtg gttaccttat atacaatgga gaccctatcg agagtatcat gtatttcaag
    11341 tcccggatac atcatgccaa ctggaacgaa agtgaatgtc atacatgcgg gaatgtcaat
    11401 ccagagcaaa tatttaatat agtagaatca agggttttga atgagtatgg acaagaaaca
    11461 ctggcccgta agatatcccc taagcaatgg gctactttct ttaaagaaag ttttcacaat
    11521 ggagataaag aaaaagggct aaacaatgac ctgcctccaa tcaacttcaa aattcccggt
    11581 aaattaaggc agtttttaat ttttatgaca agagatatct tgtcaaagct ttcaaatacg
    11641 cagtatctgg tagttacttt tttagaggcc ccacttttgg cttttattct ggcgtttatt
    11701 atcaggtatt ataacgtaga tatttctaac cagttgggtt atactttttt taaaaatagc
    11761 aatgtcccgg tttacatttt tatgtctgtg attgtggcaa tatttatggg cttgaccaat
    11821 agtgccgaag aaataatcaa agatcgtaag attctcaaaa gggaagcatt tttaaacctc
    11881 agctggggaa gttatttgtt ttctaaggca gctgtactta caataatttc tgctattcag
    11941 gctttaactt ttgttttggt cggaaatagt attatagaaa ttcacggaat gttttttcaa
    12001 tactggatag ttttgtttag tgcgtgggtt tcatctaata ttatggggtt ggtaatttca
    12061 gatagtttca aaacggtagt taccatttat atgcttattc cgtttcttgt tattcctcag
    12121 attattttga gtggtatcat tgtaaagtac gaaaagatta atcctttaat atcatcacca
    12181 agttctatcc ctttttatgg ggagataatt acagctcgtt ggggatatga agctttggca
    12241 gtttaccaat ttattgaaaa tgactataac cggcagtttt ataagtacaa caaagctatg
    12301 agcattgcca gtttcaaaaa agactattgg attatcaatc ttaagaataa gttggaaatg
    12361 ataaaaagcc attatcagcg tcctgagtat aaagatgagg tagctaatgc tttagttgtg
    12421 ttgaaaaatg agattggcaa agagttaaaa gcaaacccta aagtggtgta tatacatctt
    12481 gaggagctta cccctgagaa aattaatgat gcagcaattg aagatgcttt gacttatctt
    12541 gaacaattaa accggtatta cataaagcta tttaataaag ccaacagtga gaaagatgag
    12601 attgtaaaca tggcacaaca gaccccagca atgcgcgaac aatttcttga gttaaaaagg
    12661 cgttgtgcta atgagtcttt agaagagttt gtaacaaata ctaatgaagt ttcacgtatt
    12721 atcgaatata aaggtgaact gatacagaaa ctagacccta tttatcttga tcctacctct
    12781 ccatttataa aagctcattt ttatgctcct cgtaagatgg tttttggggt gtatatgtcc
    12841 acattttggg tcaacgtgat tgtaatttgg ctgacaacgg ttttgttgtt tttggtacta
    12901 tatttcaggc tcttgcttaa gttgttagat aaactggaag ctttgggcgg atatttttca
    12961 aagtcggaag aataatcagt gcatcagctt aaaaagtagc tcgcgcatta gctcgtaagg
    13021 tggagtattt acacttccta taccttttgt ttttaaatca tattctctca gtattgaaat
    13081 attggtgata attttctgaa ctgaataatt tcgggcagca acttcaactt cttttacaaa
    13141 gaaaacatgc gaaaggctta agcttgaagc tacatgttgt ggcgacttat tgtcgaggtg
    13201 atgataaatt aacagattag aaaagaattt aaacaaaact gaaattatta tgtagggcgg
    13261 gtttgctttg ggatttgctc ccaaatactc gataatccga ttagctttta aatggttttt
    13321 agttccaagt gttttttgca actcaaatac attatagtct ttgcttatcc cgatattttg
    13381 ttcgataagc tctttggtaa ttgttttgct gtcttttggg ataaccagca taagcttatc
    13441 cagctcatgg gcaatacggc tcaaatcttc accaatatgg tcggccaaaa gttgagaagc
    13501 ctccggtccg atattataac cacttttttt gagataatta tttatccact cgacaatttt
    13561 gtaatcaggt attttttttg tctcaaagct tacgtgctta cttgctaagg tagcaagttt
    13621 tgttcgttta tcaagtttct ttttataatt aataaccagt attgtagaag gctggggctt
    13681 ttcaaaatag gggatgagct tttcaatatg ctgaagttcc tgggcttctt taataataac
    13741 aacctggttg tttgccatca taggaaagcg gcgggctgcg tctaatattt tgtcaatact
    13801 ggtatctttg ccgtaataaa ccatttggtt aaaagttttt tcagcatctg tcaggatgtt
    13861 tcctgcaata tattctgtaa gccggtccat gtaataaggt tcttcaccat gaaaaaaata
    13921 gactggtttg tatatctttt ttttaagctc gtttaatatt tcatcatatt ctttcatcaa
    13981 tggctatttt gtttttggct atattatctt tacagcaaaa ataatcatat tctaatcttc
    14041 agaatgaaat ttccggcact taacttacct gtagctgatt ttacattgcg aaagcaaaat
    14101 ggtaagattt atgtgtttga tgtgttgcga aagaaatatg tttaccttac tcctgaagaa
    14161 tgggtacggc aaagttttct gcattattta ttgtttcatc ttcagtatcc ccaggggtta
    14221 attgcccttg aaactccaat taagcttaac gacaacaaaa tgcgctcaga tattgtggtt
    14281 tttgacaaac aagggaaacc tgcattgctg gttgaatgta aagctgctca aatagaaatt
    14341 tgccagcaga cttttgacca gataattgtt tataatatga agcttaatgc tccttatctt
    14401 attgttacta atgggttgga acacttttgt tttcggcagc cttcattggg gcaaaagata
    14461 gaatttttta ataatatccc agcttacgaa ttgattagcg gtactttggg ttaattattt
    14521 gcatgagtaa ttcgtcatgg tacccatcat tgcgtctggt ccactttttt tttacaccta
    14581 tggcctcaaa gccaattttt tcaaacagat tgatacttga tgtgtttgtg gtgtctatat
    14641 tgcaatatag ttgaaccata tctaatgttg aaaaacagta ctcgcaaatt agttttaagg
    14701 tattgaaggc ataccccttt tgtctgtctt gtttttctgc tataagtatt cctattcctg
    14761 cacggttatt aaaaatatcg taatcaaaaa tgtcaactgt gccaatggct ttgttttttg
    14821 tacttttcaa aacgatgata aagcgttgct gttttgcttc aataatgtct aaatgggctg
    14881 tttcaagata tttagaaagg gtaaattttg aaaatggcga atgggtaata cctgtttgcc
    14941 atatatcggt atcattctcc caatgataaa ggaaatctat atctgtaggt tcaggtgccc
    15001 gtaataataa taattcgttt tcgagtaatg gcatattatt gtttccagat atttgcaggt
    15061 gagttttctt tttgggggct gtctgctatt tcaatgttta taaaggctgt gtcatttttt
    15121 aaaattgatg gtacaatgat gtgcatgttt ttttcagcta tattactacc tttataagac
    15181 agctgatata taccttctgg aagactaaag gtggcaatgc cattgttgtt agtctcggca
    15241 atctttgtaa agtctttgct tgataatgag ttgatgaaga ttgaataatt tgatgccggg
    15301 tgtttattgg tcattacctt cgtttcaaac aatattgtat tgggttgttt tatattagaa
    15361 ttatttttac gtaatatatt gttgtataaa aaattaaggc tatataagtt tagcgggcta
    15421 tacgaatagt cgtcttcagg ggtattgaat ggaaaaccta aagcttcggg ctcagaccat
    15481 tgagtgtcgt caattctgta tgctttgcat atttcggctt tgttttttcc ttttgaaatg
    15541 gacatggtag aaaaaagcag acaattgtct ttattgacaa gagccggata atcttcgtca
    15601 aaattggagt ttatgacagc gccaaggttg atggctttgt tccattctcc attgacttta
    15661 acagaaaaat aaatgtccat gcctccaaaa cctcctggac gactactggc aaaatacaag
    15721 taaacgccgt cttctgtttc atagggatgt gtatcgctat atcccgaatt gatattgctg
    15781 tttagttttt tcaatgtttg ccacgtctta ccatcaaagg tactaacata tattgttcct
    15841 ccaaatgcat cgaatttggt aaagtacagc cgtttgccgt cggcgctgaa tgaagatggg
    15901 taaaaatcgc ctgttgagcc aagctgtgag gtaatatctg cagggcttaa ccattcgttt
    15961 cctttctttt gtatgaagta aatcttattt tcagaggagg taaggtcaat aaagacaatg
    16021 cgtgtaaagt cttttgatat gcagatgttg ttgtatggtt tttttaaatc aaatttaaaa
    16081 cctgaagata aattgaatgg ttttgaattt gaagtagaat cagtaaatgt gcttttttct
    16141 ccaactgtta taaaaaccgg gttctgtcca aaattgttat ctggaacatt atacgctttt
    16201 agaaagtttt tatagctgtc ttttgatttt ccggcaagag ttaagcctat ttgaaaatta
    16261 aggtacttgt tttcagggtc tttgttaagt aattgtttta aaatattggc agcttcatcg
    16321 ttttggccat taaacatgag catgtctgct ttggttagta gttttaagct gtttatttct
    16381 tcctggccat aacaagctaa tgtttttaca accataaagg ccactgaaac caataccttt
    16441 ttcattgcat ccatttttca ggttttttta tgtttgtttt ataaaagtta cttactgttg
    16501 ttgatggtaa ggcttaactt ttgtaatgaa gataaaactt ttttcttagt ttcttcgtca
    16561 tttacataaa gccctccaat aattgctttg gtatattcgt ctttaacaag gattttctgg
    16621 ggttttttat cgcttaaaag gtttttttct actgtatagt tttctatttc atatatatct
    16681 tctttaccaa agccatcttc tttggttaag cagttaaaag cttttgtccc tttgttgaaa
    16741 ggataataat ataggtcatt tccggtagta caaacagggt aacctgcgtt gataggtaca
    16801 tcccatttgt tatcgtctgt cattgttgtt gtgaaaacgt cataacctcc catgttgaaa
    16861 tgcccttcag aactaaagta taatatttta tcatcgttgc acagtatggg gttggtttca
    16921 tttgaagggg tatttacttt attcccaagg ttgacaggtt ttccccattc tccattttcg
    16981 tcttttttag acagataaag gtcgagttta ccttctcctc ctcttctgtc tgagctaaat
    17041 atcaatattt taccatcaga agaaattgtg gctgcacttt catttttgga cgaattgatg
    17101 tttttgttca gcggtttcat aaggctccat ttgttgtttt cgttcagcac ggatacataa
    17161 agatcgttgt ttcctttttg ttgttttaca atgaataatt cttttccgtc tgatgaaatg
    17221 catgtaggat aacattcgcc gtcattgcca atttgagggt tgatgttttg aggcgtactc
    17281 cagcctgtgt ctgttctgta cgaaacaaaa attgcgttgt aaaattttaa ttcagaaacg
    17341 taaataagaa tagagccttc aacgtttgtt atggcgtggt tatttgtacc ggcagtattt
    17401 ataacagaag ggagaggggt aacttttata tctaccggca gatcttggat aatctttgct
    17461 ctcgaacatg catcgatttc attgtccacc atttgagagt tgtaggtgct ttcaaactta
    17521 gggctgtttc tgaatttgtt atatgcattt aaggcttcag taagctgatt attaatgcgg
    17581 tatgcattgc ctaggtaata aagggcatat aaaggggctt ttttttcttc cagtgtgttg
    17641 gctttgtatt tagcgctaat gtttgcagat gctttttcaa ggtatggcaa tgattttgtt
    17701 tcttctcccg gtatatttat ataacacata cctatctgaa aattgatatt gtgattatcg
    17761 ggcattgctt catgcaattt aaggtaattg tataaagctt cacggtaatc tcctgattca
    17821 taaaagaaag ctgcatcttc aaaaatttct tttagttggg gttgggttga gcctttttgg
    17881 ggcttttttt gggcatgatt atttgtggca atgatcagca aaattgtggc caaaaggcat
    17941 gttttaaagg tcaatacttt catcataata gaatttaatt tttaaggaat gatagcttgt
    18001 cccgtctttg gcgggaaaac gcccatgttg gctattttgt tactattgtg tttgcgttcg
    18061 caagaacatg agtgtttcat tttcttaaat tatctgaatt ttataaaatg ctcaaatata
    18121 gagatttttg aagtaaaatc ataaaaaaag ctgattaagg caatggttgt tgataaaaaa
    18181 tggtcaaatt tcgaacccca tcaatagtac atcatcaact tgttcaatat cgcctttcca
    18241 ttgctcaaaa gcttctgcga ataatttttc ctgcttgccc ataggggtat ttgccaattg
    18301 taagataagt tcgttaagtc ttttgctcat aaactttttg ccattaggcc cgccaaactg
    18361 gtcttcatat ccatctgaca tcagataata tctgtctcct ttgcttacat caaaggtatg
    18421 ctgttgaaag tccttcatct caaaatatat agctacaggc attttatctc ctttggtttg
    18481 tattatttga ccatttttta ataaaaggag ggggttgttt gccccggcaa aatgtatttg
    18541 cattttttct ttatcaaagg caacaatggt aatgtccatc ccgtctttat tgaggttttg
    18601 gctgtcattt tgttgtaatg agcagataat gtcgtttctc aattcgttta atatttgtgc
    18661 agggctgata atgttacgtt ctattacaat tttattgagc atgctcatgc ccaacatact
    18721 cataaaagct ccgggtacac catgtcctgt gcagtctgcg gctatggtaa aggttgtatt
    18781 gcctacctgg ccaaaccaga aaaagtcacc actcacgatg tttcttgggc gaaacagcac
    18841 aaaataatca ttaaaggctt tgttcaatcc gacactttta gtcatcaatg ctttttgaat
    18901 acgctcggca tacatgatgc tgtcggtgat gtctttattt tgtgtttgta caatgtcgtg
    18961 ttgtctgcta atttcatccc gttgtgattc aatttcattt ttttgtctaa caacttctgt
    19021 agttcgttcc agtacaattt gttcaagttt ggctttgtcc tctctaagct tcttttctcg
    19081 ccaggtaacg taggtataaa atattgttaa gccaagtatc gaacatatta agtaaaacca
    19141 ccagcgcatc caaaatggag gcctgatttc aaatgaataa gttaatggtt gtgcagacca
    19201 taagccattt tcgccccttg ctttcagctt gaaacagtat tttccgggtt taagaccggc
    19261 atatactgct tcgtttgttt gtctgggagg cgaccacgta gtctctaatc cttcaagcat
    19321 atatgaatat tcaacttttg aagggttacg gagcgagatt ccgctaaatt gaagtgttat
    19381 gtggttttga ttgtaagcaa aagaaataga gtctttgatt ttaaagtagt taagtttctt
    19441 gcttgttgta tcggcataat aatctttaaa gaatagtttg attttttcaa tttgcaagtg
    19501 gggggtgctg gtgttggcct cttcaagttt agggttatat ttggtcaaac attttacagt
    19561 acccatccat acttggtttt gtctgtcttt gtatattgaa ttttgaatat tttcttctcc
    19621 tataaatcca ttagaacggt catatttaaa aaactgtttt gctttcttgt gttcatctaa
    19681 cgaaatttta accattcctt tgtctgtgcc acaaaggagc atttggggat cataaaaaag
    19741 caaagaatat atggaattgc aactaaggtg cgagctgttg gctacttcaa taaatggttt
    19801 ttttactgtg gtatctgggt taatccggta taaagttcct cccgatgttc caacccaaat
    19861 attaccgtta taatcagaag tcagtgtatt tgtttttttg tcaaataatc cttcttgttc
    19921 atcgtagctg atcatttggt tgtttttcat ttttatgagc ccgtctaaag tggcaatcca
    19981 gacggctttg tttttatctt ctgcaataca ttgtatttca ttggtgctaa aaccatattg
    20041 ggtaaggtca aaagaaaaga atttatcgtc tttaatgata ctgatgccgc ctatggttcc
    20101 tacccagatt cggttttggc tgtcgttgaa tatgcaaaca acttcattgt tgattaaccc
    20161 tgtgtttgaa ttgtaaattt ttaaactgtt ccccctatag gcaaaaacgc catgagagcc
    20221 tgtgccaatc cataaatagc catttttgtc ataaagcatt gaattgattt ttaccccgcc
    20281 taaacggtca aatggcttta tgacaggttt atttttgttg aacgccaaat gatatagccc
    20341 gtttgttgtt cctaaataat aagtgccgtc tggttgctgg ataatgctgg taacattgtc
    20401 gatcaaacca tcgtccttgt tgaaatgcga gaaatgatcg cccagaaatt gaattagacc
    20461 gcctgttaat gttccatacc ataagttttt ttcattgtct tgataaatgg tataaataaa
    20521 atcagatggc aaacctgctg tggtacccat gtatgaaatt ccatcagggt ataatctgcc
    20581 tataccagca ttatttgtgc caagccataa gttattctgc ttgtcagtaa gcactttcca
    20641 tacctgcatg tcatcagtgg caggtgggta ttgctttatt tgagatgagc ttgtaatttt
    20701 gtctgtttta ctgttcaatt ggaatactcc tgcattgtat gtgctgaaaa atacttgtcc
    20761 ggagttgttg ctccatgtag aaagtatatg atttgaaggt ataacaggtt gtgaaatatt
    20821 ggtgacccct ttagaatcaa ttacataaat accataaccg aaacttccaa ggtaaatctt
    20881 atggtttgct tctgcaatgc caaaaatatg aggggcttca attgcagaat cggcgttgta
    20941 atggacaaat gttattttgt tgttagataa tgaagcctta taggctcctt gtccagatgt
    21001 ccctatccaa atattgtctc gtgaatcttt gaaaaacgta taaatagtaa gagtgttagt
    21061 atcttttatt ttggcataat tggtgaaatt tttcccgtca aaaatgctta aaccgttatc
    21121 tgtccctacc caaatcctgc cgtatttatc ttcagtaata gctcttatcc agttgccggg
    21181 caatccattt ttacgggtgt aatatttgaa tgtcaatccg ttgaaattgc aaagtccggt
    21241 ttcggagcct atccaaagta cacctctcga atcattgtat atggcgtata tgttagagtt
    21301 gggcaaaccg gcatctatac tgtaactttt gaaattcctg tattgtgcgg ttaacacggt
    21361 attaaaagca attataacga aaaacagtat tttcaatact gtttttgaaa atactgtttt
    21421 tcccattttt gacaaattta atggatgatg caatttgttt attttttgat aaagataaat
    21481 tgtccgcctt cgtcaccagt atctttgata ggttgaaagt tgggtgactg agaagaattg
    21541 tttactaccg gtattttgat agcatcaaac aactggcttg cgtcatacat attgttgttg
    21601 ttgttgctca atgttttgag caaataatat gcaaaaacag agtgcccgtc ttttccgcca
    21661 tccataacag gctcaatacc gcctgatgta atagctttgc gcgaggccag gttataaact
    21721 tttgtatagt atttttctgt ttcttcaaaa ggtatggtca tggttttgcc cctgaaaatg
    21781 tcgccactga aacaagcgtc tgaaatcaaa agtgtatgtt ttgactttat tcctccaaga
    21841 taggtctgga tatcattgtt ggagatacat tgtgaagtgg aattggtggt agcatcaata
    21901 gggacccaaa aacctttgtt taattcttgt ttgaaatcgc catgtccact ataataaatc
    21961 aatacgttgt cgttttcttt tacactttta ataaggtttt ctaattcttt aatgatattt
    22021 tctcttgttg cattttcgtt atataatgat ctgaaactgt caaatttata ttttttcttc
    22081 aataggtctt ccaatgcttt tgcatcgttg acggcattac tgagcttggc ccattctcct
    22141 ttgtaattgt caataccaat gattaaagca taataattac ctacttgcat ggtctttttt
    22201 tcctgggcta cattcaaacc tttcaatggg tcggtgcttc cacggtaggt aactttttgg
    22261 tctgtagtag tttctttaac aacgtattcg cgggttgtag tgcttgtcga aatttctttt
    22321 gggctgtctg aaggttgaga cgaggtagca gattgctgag ctacttttgg ttgttccaca
    22381 tcttctgcca tttttttatg ggtaacagct tttcccaaaa gtttatcagt ttgttcacag
    22441 gcgtcaatca tttcagtatt actggctaca ttggtgaatg attcgttttt taaaaggtta
    22501 agggtgattt ttaccttgtc gttcatgtct tcaatttcgc cataaaggat atgtgtaaat
    22561 gattctccgg acttttcaaa taaagcctgc gaaaaagctt cattgacctg agggtcgttt
    22621 tttttgctta ggttccaccc tgagctgttt ttggcttgtt caaccgctga ctggtcaata
    22681 agtttaaatt tgcctttgtt catttttatg gctgcaatgg ttttaagata actgtcatat
    22741 tgtttataat ctttaccatt gccttgaaaa cccaaaacta caacttttgc tgtggttttt
    22801 acttttgaag ctgcaataag cttgtcaaac atgttgaaca tgagggtatc aaagttgcct
    22861 gtttgggcat tatttatccc gtaagtcagg cacaataata agattaaata ttttttcatt
    22921 gttaatgatt tttaaggttc gttattgtta gttaagtatc acaaatttag gttaattact
    22981 aaagctttca aagcaaaaag tacagtttct taatcaggta ttgtactttt gaacagtaaa
    23041 tttaaaaata ttttccgatt ggagtctaaa attgctttta aagacattct gagaataagc
    23101 cttcccatta ttggcgggag tgcagttgag aatattacag ctattatcaa tactgcattt
    23161 ttgggtcatg tgggtgctgt gcagcttgga gctgtggcat tggggggtat tatgtacctc
    23221 atttttgtca tgcttggttt tgggttgggt attggcatgc agattattgt tgcccgaagg
    23281 tatggtagca agcagcttga taaaattgga gaggtgctgt atcatacttt ttttattatg
    23341 ttgctattgg ggcttgcatt tcttttggcc ggggttttat ttggaccttc tttgctcagg
    23401 atgattgtga aatctgacga gatatacttc aatcttcatg catttctttc atttcgtata
    23461 tgggggattg gttttgccta cttaaatatt gttttcaggg gcttttacac cggtatcatg
    23521 agaactaagg taatagctgt ttatagtatt atcctggctt ctgttaatgt gttttttgat
    23581 tattctttga tttttggaca ttttggcttc cctcaaatgg gtattgctgg tgctggcttg
    23641 tcttcagtta ttgctgaatt tgcagccatg agcttttttc tgatttttac attgtttcac
    23701 aaggaaatca aagcgttttc tattacccgt ttcgcgggtt tcaaaaaaga agtgcttttc
    23761 aatcttctga aagttgcatc gcctgtaatg gtgcagtttt taatttcatt tggaggttgg
    23821 tttttctttt ttaccatggt cgaaaatatg ggagaggttc cgttagcagc ttctaacttg
    23881 cttaggacat tttacatgat tgctttgctg cctctttggg gcttttcatc tgcaaccaat
    23941 acgttagtca gttatttgat tggtagtgga aggattggag aagtaaaaaa actggtgttg
    24001 aaaattatgc tgttgagttt ttccagcgtt ttgatgcttg ttttgattat caatatcttt
    24061 tcggctccgt atctggccat ttttaccaat gatattggcc ttatacgagc ttgttttccg
    24121 gccttttaca ttgtcggcct gagttcgctt ttgctatcac ttactatagt attttttaac
    24181 gtgatttcag gagccggaaa aactacggtt agcttattta ttgagttttc ggttatcttg
    24241 gtttatatct cctgggcttt tctggtttct catgtttggc attcgtctgt tgccatgatt
    24301 tggttggctg aggcatttta tggatttaca atgggaagcc tagccttgtt gtatttgaaa
    24361 tttgggaatt ggagaaatgc caagatctga ttaccgtgct tttctgataa tcatctggca
    24421 gtttttgcag tcgaaaaata cttccagcct gtttttgtgg ctttccaata ttaacgtctt
    24481 ctctttagcc agtaaagaat tggggtttat cttggggcaa gctccaagtt catatttaat
    24541 gcaatacctg ttggtcatta aatcggcaag gcttgattta tctgacattt cagccccagc
    24601 tgctggcgaa gcaataccat gtctgacgta aaaagctttt gaaagcttat tggtgatgtt
    24661 cagtttgtag ctgttttcag ttaaaggaaa aggtgtgttg ttagggatga tttgatattc
    24721 gagtttttta aagtttttaa ttctttcttt ctccaataat tcgaggcatt gccgacgcat
    24781 ctcgttagat tgggctgaag taaaaaacaa aggttgggaa aattgaatgt ccacattttt
    24841 cacttcaaaa ggtgtgttcc cggacttttt caacgtgttt ttcaaccctt caaaagcttg
    24901 ttctgcttta gtcgcaatat ttttttctat atcaaaatta atatttacaa tattgttgtc
    24961 ttcgtctgat gattcaatgc aaagtccggt tggggtttct gtaaccttga tatctgcttc
    25021 aatctttctt gtttggatag acttttcgag ctttttctcg aattcttcat cgtggttgcg
    25081 gtatagaatg gtaccgtcag gaatgctaat gttttcattg ggttgcaatc tgctgccttc
    25141 ggcaatatta acccgaaagc cttccattat gccgtctttg ttgataaagc aaagcccatc
    25201 gccattgctt attttttctg ataattgggc ttgtatccaa tttcctttgg tattaataat
    25261 gtggccaact tcttttccta aagatttagg gctgtataaa gatgccatgt tttctgttcg
    25321 gccatttata aagtattgag taaagccacg attgaaactt ctttcagggt caggtgtgaa
    25381 attgatgatg ctctttccag atgaaggttt agtcagggtt tgatggcttt cttcaatgat
    25441 tttatccagt tgttgcctgt accatgccgt aatgtttttt acataagcaa tgtttttgag
    25501 ccgtccttca attttaaagg atgtaactcc agcatctatc atgcttttga gcttattgtc
    25561 caacctaagg tctttgggcg aaagtatgtg cttattcttt tcgagcaatc ggccatcagc
    25621 agttttaagg tcgtacaacc agcgacaggg ctgggcacat tcgccacggt ttccgcttct
    25681 gcccgtcagt acttggctca tgtaacattg tccgctgtat gatacgcaga tagcgccatg
    25741 tacaaaagct tcaagttcta tattggtact ttttctaatc ttagtaattt cgcccaaact
    25801 aagttccctg gccagaatag ccctttggat gccaattttt tcaagaaaag aaaccttttc
    25861 tggcgtattg ttgtgtgttt gagtgctggc aaaaaggggg atagggggca acttcatttc
    25921 aagccatgcc atatcttgta taatcagggc atcagctcca atattccata atttattggc
    25981 tatttcctgg gcttcttcaa gctcattatc aaacagcagg gtatttagtg taacataaac
    26041 acgggcgtgg taaatatggg catattggat aagcttttca atgtcttcta ttgaattgcc
    26101 tgctgctgcc cgtgccccat actttggagc gccaatatat acagcatctg caccgcagtt
    26161 tatagctgct ttgccaaatt cgaggctttg tgccggtgat aataattcaa ttttattcat
    26221 tgaataacag aaatttgaaa aatcccaacc gacaaaccga caaaggcttg gaatatttat
    26281 cgtttgagca ggatatagtt cttaaattcc cacataggcc taaagaaaac atatttttca
    26341 agaaaagtaa tgaaacggta tttgcgcttt tcgtgtttcg ttaactttct gtcttttggc
    26401 atttcagtga cttttgcaag ttcatctttc caaaaaagct tgtcaatcca tgattgcata
    26461 agctttggat gagttccttt atagactgga attttgctca ttacgccgta gtcaaatctt
    26521 tccaattgaa tctttttttg aagctcagcc ttggattgta tctcgctgaa atctttaatt
    26581 tttttctgca tatagttcaa tggcctaacc catccatagt gaaatatccg ggcatccacc
    26641 ttggctactt ttaattttga ggtattttct tcctgtctgt aatttttttc gtcaaatccg
    26701 ggaattcgcc tgaaagattg agcgtcgcgc catgagtgga tgtctttgtc attccggatg
    26761 atacggattt catttttgta ccagccatgc gaatcgatat ggtggtcgta atcgccccaa
    26821 aagtggaaat atctgaaaag caagccttct atctcgttgt cattcaataa ttcttcgcac
    26881 cgttttttta tcttgggcaa gtcttcctca tgaactacct cgtctgcctg gatgtaaaac
    26941 agccaatcac ctgtgcaata agattttgct atgtcagttt ggtgtgcaag ttctgtccct
    27001 tctggatatt tttcaatatc ccagacagtg tcaattattt taatttttgg gctattgatg
    27061 ctaagaattt cttctcgcgt cttatcatcc tcatcacaat ttccaagtgc tacaatgaat
    27121 tcatcacaga tgggaaggat ggataaaatt gattctttta tagggtaata gagttttgtc
    27181 acattcttac ccatggtaaa tccgctaatt ttcatagata caaagattat tcagctgaaa
    27241 acatttgcat atcgttcaat ttcttataat acccgttcaa agcaaggagt tcgtcatgct
    27301 ttccacgttc tacaatctga ccttcatgca acacgcaaat ctcatcagca tgtacaactg
    27361 ttgaaagcct gtgtgcaatg acaatggtag tacggttttt catcaggttg ttaagagcat
    27421 cttgcaccag tctttcgctt tcagtatcaa gagcagaggt agcttcgtca agtatcagta
    27481 ttggtgggtt atttaaaact gcacgtgcta tacttaacct ttgacgctga cctccggata
    27541 attttcctcc tctatcccca atgttatggt gatattgatg atttgttgac gtgataaagt
    27601 catgtgcatt ggctacttta gcagcagcat atacatcttc ttctttgatt tcgtcccgtc
    27661 caaaaattat gttattgtaa aatgtatcat taaagaggat tggttcttgg ttaacgatac
    27721 ccatcaggct gcgaaggtct tttattttaa ggtcttttat gtttataccg tcaatcaata
    27781 tttcgccatg ttcaacatcg taaaagcggg gtaaaaggtc aaccaacgtt gacttgcccg
    27841 aaccagattg gcctaccagg gcaacagttt tgcctttttc aatacgaaga ttaatatttt
    27901 taagtacttc gtgcttgtca tacttaaatg aaatgttgac gtattcaatg acactgtcaa
    27961 attttttctt ggatactgca tcttttttct ctttgatttt gttttcagct ttcaggatga
    28021 aatctatacg ttctgccgaa gctaaacctt tttggatgtt gtagtatgca gaagaaagct
    28081 ttttggcagg ttctaaaatc aaataaaatg ttaccaaaaa tgccaataag cctgatggtt
    28141 gcatgtctat ggatttatta agaatcagga cagccccata aactaatatg ataagcataa
    28201 ccactgtgcc taaaaactcg ctaactgggc ttgccagcga acgtctgcgt gtaatacgtt
    28261 tattcatctg ggcaaaatcc tggttcattt ctttaaacct tttattaata ggtttttcag
    28321 cattgaaagc ttttataatc cttaatcctg aaatggtttc ttcaattagg gcaaggatag
    28381 aacctaattt gttttggctt ttgtatgatt gttttcgcaa tgtcctgcca attcggccaa
    28441 taagcaagcc gcttattggc aacataatca aaacaaagac agttaaatgc acactcatag
    28501 tcagtaatgt agccagatat atcaatatta acagcgggtc gcgaaaaacc atttcaatag
    28561 agctgattac agatacctct atttcctgaa catcagatgt catacgcgcc ataaggtcgc
    28621 cttttttctc atttgagaag aaagaaaggg gcaactgcaa tattttatca aaaagcttat
    28681 tgcgtatgtc gcgcagcata tatgcacgta caggggcttg taaatgctgg gcaagatagg
    28741 caaatgtgtt tcttaacaaa gagaaaaaag ctaatgcaaa agcaacaatt agcaaagtct
    28801 tatattgtcc atattctgtg attaattgcg caaaaatgta atcaaaatgc tttttgtatt
    28861 ctaaaatgtt aaaatctccc ggagggacag tagaaacagg ctgtgtagaa aataaaatgt
    28921 caaaaaacgg ctgaaggaac atgatggttg aaatggtaaa aaccccggat agtaagttgc
    28981 ataaaatgct tagggttact tttaaccaat aaggtttaat atattcagca agtgagagta
    29041 tctttttcat gagtgtttgt tgttagtgta ttctttcaat tatatcccgg tataattaaa
    29101 aggggtaata gcgagcaatt ccttttttac agcatccgaa acttcaagac tggtcacaaa
    29161 ttcttttatt gaagcttcat taatactatt gtttcccctg gtcaatagtt tcaaggcgtt
    29221 gtatgggtca ggataattct cgcgacgaag aatagtctga atagcttctg ctacaacagc
    29281 ccagttcttc tcaaggtcat tttttatagc ggtttcattg acaataactt tgcccattcc
    29341 tttttgtagt gattttaagg ctattactgt gtgtgataat ggaaccccaa catttctaag
    29401 cactgtagag tcagttaaat ctctttgcat cctcgatatt ggcaatttgg ccgaaagatg
    29461 ttcaaagata gcattggcaa tgccaaagtt accttctgca ttctcaaagt caatagggtt
    29521 aaccttatga ggcatggccg aagaaccaac ttcgtctttt ttcacctttt gtttgaaata
    29581 ttccatcgat atatatgtcc acatatcgcg gcaaaggtcg ataagtatgg tgttgattcg
    29641 ctttaaagca tcaaatgtag cggcaaggtt gtcgtaatgc tcaatttgag tagtggtttg
    29701 tgacctgctc agtccaaggg tttcattaac aaactgattg ccgaatttta cccagtcaat
    29761 ttgagggtaa gcaacaacat gggcgttgaa attgccggtt gccccgccaa acttagcaga
    29821 ccaaggaatt tgccccagtt gttttttttg tacttcaacc ctttcaataa atacttgtat
    29881 ttcttttccc aagcgggtag gagaggctgg ttgcccgtga gtatgggcaa ggagcggaat
    29941 tttgtcccat tcatctctta cttcacagag ttttgatagt atttgattta ataaaggaac
    30001 gtaaacttca ataacagctt ctttgagaag cattggtgta gctgtattgt ttatatcttg
    30061 cgaggtaagc ccaaagtgga taaattcttt gtattgttct aatccgagtt tgtcaaagta
    30121 tttgcgcaaa aggtattcaa cagccttaac atcatgattg gttgtttttt caatgttttt
    30181 aacctccaat gcatcttcaa tagtgaagtt tttgtagata tcgcgcaaag caatatattt
    30241 gtccttattg aaatccttta gctgcggaag gggcaggtta cataaagcaa taaaatattc
    30301 gacttctacc agcacgcggt atttaataag tccaaattcc gaaaaataag gagcaagttc
    30361 agaaatttta tctctatatc tgccatcaac tggcgaaaca gcggtaagtt cagttaattt
    30421 catcgttgtt tattaaaagg acaaaagtag aataaaaact atattttagt taaaaatcat
    30481 ttgccggaat ttgaatttaa atgttcggca gcaattttta attgttcata ccattcatta
    30541 tcaaaacgtt gaattagtgg ctctttcaaa aaaacgtaaa gaggtatgtt ttcattgtta
    30601 ccttttataa gcgcattttt acagatatcc catttgtggt agtttacagc gctaaaagat
    30661 ttgtatttgc ttattcttac cgggtataac caacatgaaa ttggctttct taatgaagaa
    30721 tggccgtttt gccaggctat ttcaataccg cattgtgcaa tgccgttgtt gaaaatggca
    30781 aacgcacatt cagcgccatt aataagcgga gtaaccatgt cgccgtcagt atcaataacg
    30841 taaactcctt ctttatcaat ggttttagct ccttcttttt gtaaataagt ttttattttt
    30901 tcaatatcgt tctttatttt attggcttct tcgggttcta aaggggcgcc tgaatctcct
    30961 tcaatacaac aagctccttt acattgaagg atgtcgcata caaatttttt ctcaataata
    31021 tcaaggctta tcaacgtgtc gtttatctgg agcatattat cttttttgca aaattacgca
    31081 aacgcagata gattacctcg aattgttttt tgattaaagc aaatattttt attacatttg
    31141 agtatatttg taaaactttg taactaataa tggctaaaac tcaggacaaa aagatttcgg
    31201 ttattcttgt ggacgatcat caattgtttc gcaacgggct gaaatttatt cttggcgaaa
    31261 ttgaaggcct cgaggtggtc ggagaagctt caaacggtca cgagtttttg cagcttcttg
    31321 agtatgtaaa gcctgatttg gtgattatgg atatcagtat gcctggcatg aatggcattg
    31381 atgcttgtcg tcatgctttg agcaaagacc caaagctgaa aatccttgtt ctttcgatgt
    31441 atggtgatga tgcttattat aacaccatga tagaacttgg ggtaaaaggt tttattctta
    31501 aagattcgga caataaagaa ttacaaaacg ctgtcaggac tattctttca gggcaaaatt
    31561 atttttcgca ggaacttttg ctcaagatta ttcgtaataa aaatgtaggc cctgcagtaa
    31621 agcttagcaa acgcgagaat gagatattgt cgcttatctg tcaggggatg tcaaccattc
    31681 agatttctga aatgctgcat attagccatc gtactgttga acggcatcga gccagtttgc
    31741 ttgaaaaaac agattcttcc aattctattg cattagttgt atatgctata aaaaacaatc
    31801 tggttacatt aaacaagtga cggtttttac ccatattaaa aataagtatt tctttagttg
    31861 tatttgagtg tctctttctt aaaattgggt gattttcctc ttttattcac tttcttattg
    31921 agtcattttt gtaataccca taaaatgcgt ttacaaataa ttgacttatg aagaaactta
    31981 atgtgttgct tgtagatgat aaccaacatt tcagacaagc attgaaatac atggttgaag
    32041 aatcaaataa agagaaaatt aacaatattt atcaggcagg aagtggttct gaagctttga
    32101 atattcttca aatgcagcat attgaccttg tttttatgga tattgatatg cctgacatga
    32161 atggcattga aacaacccgt aaagctactg aactgaacag gtttttaact attgtggcat
    32221 tgtcgtttca tcaggaaatg caatatatta tgaaaatgct tgaggccggg gcccgtaatt
    32281 atatcatcaa agaagatatt tgtaccgatt ctatcaaaaa agtatttgat acagtggcgg
    32341 tcaattagtc atagtttgtt ataaattggt aaaaaaaata tccccttatc ttttcaaatg
    32401 gataggggga tatttttatt tagctacagc ttatcaggct acagtattat agcttttttt
    32461 tcgtatcatg taaatcacca atcctaacat aagaacgaag aagattgata ttaatgcaat
    32521 atttgttgca gtaaagaagc ttggtttaaa cttatgaggt tcaaactggt tcatttggta
    32581 aatatttttt gttccaatct gtgcaaaggt gcgaataatt tcatttttaa cagaatcatt
    32641 gtttacaaag aggtaattta ctatctctcc aatggtgcta aagttgtttt tctccaattc
    32701 gtcaaatgat acattttctt ttatcactct tgaagagaag tgttttaagt cgctaacaac
    32761 ttgtttgaga tattcttttt cgttggcttt gactaaggca aaaagtatga ttgaatcatt
    32821 gcttactccg ctaagagctt tttcatgcag gaatgagcca aggctttcta tgttttttaa
    32881 ctgtccagcc agctttttat tgttttcaaa aaaatctttg ttatgttctt cagtattcat
    32941 tgccattcca tttaatagta ctaatggaat atttgtgaaa ttgggtaatg caaagaaggc
    33001 tgaatagata tcttcttctt tataagaatt tttatctttt ttcgcgtata aataccccag
    33061 tagctgagac cctgtattga ttttttgttt tgataaatca acagaatcaa gggttgcccg
    33121 taggtttccc gaagtaactt tcttaaggtt ctgcaaataa taataagcat tgccttgcca
    33181 agctaaacgt gctgtatttg aaatcaactg ttgataatcg tttgagttat ctattgtgtt
    33241 gtaaattagg taatcagaca attggttgac agatgcaaat ttctttacag ccgaagagtc
    33301 ggcaagttgt ttcagcctgc catcagatat tttagagagg gctagtgcat aactttgtgt
    33361 cagtgcagca gctatagagt cgaaatgttt taaagcagaa tcaaccagag gggttaaagc
    33421 tgaacagctt tgttttgtct tcaaaaaatc agacaattca acgtacgaag taaaattttc
    33481 ttcagtatca agctcggcaa gacattcttt ggcagaatca ggcaacaaga atggtaacat
    33541 tcgggcagaa tatttcattt ctcttttttt catggccaat tcggctgccg attgagcttt
    33601 ggttgtatca acaggggtat aatatataat tatggtacct gtctttttta tcccttcagg
    33661 ggtggtagca ttgaatataa tttcgttttt accttctatt ggtttatact tataaatttg
    33721 attgtcgttt ttgagtttta aattctcatg tttagattct tttttgttgt tgatcatgat
    33781 gccaactgag gttcctgctt caagtggcat aataatattt ataactgagt cattctcaac
    33841 tttataaaaa gttttgttga attctattgt ttcaggttta ttcgaagtaa cttgtgatat
    33901 ttcagttttt acaacatctt caggttttag ctctgaggta acattgacct ggctctcttt
    33961 ttgatttttg tcaatagtaa aattttctat attttttttg aaaccatctc cttctacaac
    34021 catctgataa ttgccggcag tgatttctga aacaaagttt ggatttgatt ggtcaatgct
    34081 aaattgttta acagtatctt tcttagtttt gttgattaat gaaattttaa tttttgaatt
    34141 gttttcggca tgtcctttta aactgatcat accgtttagg ataaacttac gcgggtgttg
    34201 gtctgaaaat aattctactt tatagatatc ggtttttcca aaattgtcag ggtaataacg
    34261 ggttatgtat gcaaaatctc cattttgaac aggcataaaa aataagtcat catccggggt
    34321 gtttagcgga tatcccatat ttaaaggggt tgaccatgtg ccattgctaa gcagcgtaga
    34381 ataaaagatg tcataccctc ccatattgta atgcccgtaa gagctaaaaa agagaacttt
    34441 tccgtcttgc gataaaaacg gggtttcttc attaaattct gagttgacat taggccctac
    34501 gttgcggatg tttgtccata atatattatt ggtgtcgttt cttgtggcgg tataaatgtc
    34561 aagtcctcca aatccaggtt tacggttaga ggtaaaaacc aatgtttttc catcagccga
    34621 caaagaagca tgtgattccc agtattttgt gtttatgttg tcaccaaggc gggtaagcgg
    34681 tgtccatttg ccatttacca agcgggtaac gtaaaggttc ccatcataat tgtcatttct
    34741 gtatatatat aattcggttc ctccgtatga aagcccggta gcataaacat ctccgtcaac
    34801 gcccaggtca gggatgatat taataggggc agtccagcgt ccatttactt tttttgaata
    34861 gtacaatgca tcgtaaaatt gaagttttac attgtaaacc agtgtttttt catcgcctga
    34921 cactacggca tttttttctt caaacctgct gttaatcggt tctccaacgt tgttgcgtac
    34981 aaagtatatt ggccttgaca taaggttgtt ggccctttca atacatttta tctggtcatt
    35041 aacaaggtta atatcgtaaa tttcagaatc gttgttactt ttactcttaa attcatcgta
    35101 agtttctaaa gctttttcaa acatattgtt taccctgtaa gcattcccaa gatagtacaa
    35161 tgcatccaac ggggcttttc tttctttaaa actattgggg ttgtaattag gagaaatgcc
    35221 tttaacggct ttttgtaaat atccgatagc cttttctttt tcaaatggga tattgaggta
    35281 acacaaacca actttgaagt taagattgtc attttccgga tcagcctttt gaagtttcag
    35341 gtataaagga agggcttctg aaaattcttc gtaaagtaaa taagattcag cttccagaaa
    35401 catttcgcgt gggttctggt catctttttt ttgtcctgaa atacttattg cggtcagtat
    35461 aagtacacag cttagtaaaa aacggttcat gaatgtataa tagttagtgt caggttatgc
    35521 aaactaagtt taatattatt gtttattaag cctaataact ctgccttttt atgtgttcaa
    35581 ccgcctcaaa agagtttatt agaaagcttt taacaaaaaa tattgaacaa atatatgata
    35641 ttatgataaa taatttgata tataactttt attttaggaa gttatcttca gcttgaaaaa
    35701 aagaaaaccg gcatttctta aacaagaaga tgccggtttt tacaagggaa aaaggagatt
    35761 ttaaactttt tcaatggtct tttcgtgaac ttcgttggcc ctgccagaag ggtcttcgtt
    35821 cttttgccat tttgggatcc attttaatac agatttagca gcactttcag atggataata
    35881 tttttcaaaa atagtcctga aataaaaagc ttcttttgta gccggagtgt tgtgagggaa
    35941 ccttttggcg gcttcagcaa attcagcatc agatactttg gcattgcaat gagctaccag
    36001 gctgtcaatc cagctatagc ctacgccatc agaaaattgt tctttttgac gccacaatat
    36061 ttcggctggc aaataggggt cgtttttgtc gtcaaaagct gccctaagta tgtatttctc
    36121 aggtttgccg gttgatctat cgggctgttt atcttcgcct ttaatgttca tcacggcatc
    36181 aagaaaatac ctgtcgagaa atggaacgcg aacctccaat ccatgtgcca tggtcgattt
    36241 gtctgctctt aggcagtcgg cagttgacaa caggctgacc cttcgaatgg tttctttggt
    36301 aaactcttcg gctgacgggg cattgtgaaa ataaaggtat ccgccaaaaa tttcatctgc
    36361 tccttcgcca gagagcacaa cttttatacc catgtctgta atatattttg ataagaaata
    36421 cataggtgtt gaagccctga tggtagtaac atcatacgat tcaaggtgcc aaatgatttt
    36481 ttcaatagca gcaatacctt cttcaatggt aaaggttaca tcgtgatgaa tggtaccaat
    36541 aaaatcagct actttacgtg cagcaaccat gtcagggaca tctttattta gcccgacaga
    36601 gaaggagtga agcttttggt ctgttccacg catcagacgt ttggtaatag aagaaatcag
    36661 gctggaatca agccctccgg acaataaaac gcctaacggg acatcactca taagcctttt
    36721 tttagtagct tctgttaaaa aatggcgtat ttttttaagg tctgcatttt ctgtacattg
    36781 gcggtaatcg tgatattttg gggtgtaata ttttaccaaa cctgtatcgc cactataata
    36841 gtgcccggga ggaaaagctt cgaaccattc acattggtcg gacatagttt tcatttctga
    36901 tgaaaaatat aagttgcctt ctgcatcttt cccgtaatag aaaggtttaa ccccgatagg
    36961 gtcgcggctt gccataaact tgtcaccgtc aacaattaca aaagcccaat cgccatcaag
    37021 ctcattgcaa aaatcgtaac catatttttc gtacaaatgg acaataacct cgctgtcaca
    37081 ggtggtatga aactggtggt ctgcaagttt tgtgtttcga aggtgcatat agttatatat
    37141 ctcaccatta tgtacaacgt atgcattatt ggtacctttg attggctgac gccctgtggt
    37201 taaatccatg atcgataaac gttcatgggt aagaatgtag ccgttgtcaa attgaactat
    37261 cccactttca tcaggccctc tgtggctcat tctttttgaa agcttttttg cgctttctaa
    37321 atcttttttt cctattattg ccaaaatacc gcacatgata aataatttta tatatttaaa
    37381 aagtaattta ttttaaactg taattaaaaa atgtttttta aattaaaaaa tgaattaaaa
    37441 acagtgtaaa agtataaata tgtaacgaaa aaaccaaaat aaagtttaaa aaatgatgaa
    37501 aaaaatcatt ttttgttaaa aaatgaaata ttttaaggtt gaaaattgaa atagtaacaa
    37561 aagtttttaa ttatggaaga aattttacag attgatagtt tggacaggaa aattctatcg
    37621 attattcagg gaaatgcccg ggttcctttt ttagaagtag cacgtgaatg tggtgtttct
    37681 ggggcagcaa tacaccaaag ggttcagaaa cttataaaga ttggggtcat tactggttct
    37741 gagtttattg tagatcctca aaaagtaggt tatcatactt gtgcatatat aggtattttt
    37801 cttgaaaaag cagggttgtt tcgtgatgtg cttgacgagc ttaaagatat taaagaaatt
    37861 gtgcaatgtg attatacaac cgggaactat gcgatttttg ttaaagttta tacccgagac
    37921 aacacccacc tgaaaaatat tatagcagat aagatacagg caattagagg aatatcgcgc
    37981 accgagacat ttatttgtct cgaagagtgt ttcagcaggc atattcctgt attgtagttt
    38041 gaataattac ttatttttgt aaaacaaatg tattggttaa aagactgtgc aataagctct
    38101 ttgcttgttg atactttttt ttgacaatat tattgaagta catttttata ttcactaaaa
    38161 ttgtagcctg aaatttaaca taaaatatca atggcaagag taatatctat tgcaaatcaa
    38221 aaaggtggcg taggaaaaac tacaacagct atcaacctgg cagcaagctt tgcagtgctt
    38281 gaaagaaaag tgttgttgat agatgctgat cctcaggcaa atgctacttc tggtacagga
    38341 tttgagacca agagtattaa aaccagtata tatcaatgta tcattgatga agcagaccct
    38401 tcaagcatta tccttaatac agaaattgcc gggtttgacc tgataccttc gcatattgac
    38461 ctggttggag ctgagattga aatgctcaat atgcccaatc gcgagaaaat gatgaagcag
    38521 gtgattgagc ctttaaaaga taaatacgat tatatcctga tagattgttc gccatcttta
    38581 gggttaatta ctgtaaatgc gcttactgcg gctgattcgg ttattattcc tgtgcaatgc
    38641 gaatattttg ccttagaagg tatcggaaag cttctgtcca cggttaagat tattcaaagc
    38701 cgcctaaacc ctgcacttga aattgaaggc tttttgttga cgatgtacga tccgcgtttg
    38761 aggctttcga accaagtggt tgatgaagta aaaaagcatt ttcaacaaat ggtttttgaa
    38821 accattgtcc aacggaatat taagcttagc gaatcgccaa gttttgggaa gccttgcgtt
    38881 ttgtatgatg cagaatcaaa aggtactttg aattatatga acctggcaag ggaaattatt
    38941 caaaaaaacg aatctgttaa gataaaataa cagtattcat ttcggtttta attttacatg
    39001 tgattattat tgtcgcgtat attgtattgt tgtatcgtta aacatttaaa ttggtattat
    39061 ctcaaaatat atgaatgcaa agaaaaatgc gctgggaaga ggattaggag cactgataga
    39121 agaagcaaat ttaccacagg aaattgttca tgctgttgaa gcagacagtg aaatagaagt
    39181 atcgcttatt gatgttaacc cttaccagcc ccgaaaagat tttgacgaag aagcactgca
    39241 agagttgtct gattcgatca agcaaattgg gataatacag ccgcttactt tgcgtaagat
    39301 ggataatggc cgttttcaga ttattgcggg tgagcgtcgt ttcagggctg caaaaatggc
    39361 cgggttgaaa aaagtgcctg catttatcag gatagctgat gatcagggaa tgcttgaaat
    39421 ggctcttgtt gaaaacatcc aacgggcaga ccttaatgct atagaaatag ctatttcgta
    39481 tcagcgcctg atggaagaat gcaaccttac ccaggaaact ttgagcgaaa gagttggaaa
    39541 gaaaagggct acagtagcta attatcttcg gttgctcaat ttgcctgctg agattcaggc
    39601 tggtcttcgt ttagacaata ttactatggg gcatgcacgt gcattgcttg cggtagaaga
    39661 taatgaagtg aaattagata tttttcacca aattattaat gaccagcttt cagtgcgtga
    39721 tgttgaagaa atggtacgaa atatcaacaa tccgcaacaa gtacaggaaa aaattacttt
    39781 aaaagcacct aaagcagcta catccacttc agaagattat gatgagttgc gcaaacagct
    39841 tagtaagcgt ttcaatgcaa aggttgactt taaacgcaat gctgaaggtg atggcaaaat
    39901 aatcatcaag ttcaggaatg atgaagaact tgagcaaata ttatcaattc tggatacaaa
    39961 ataatacacc tgttgctttt cgataatttt aaaataaaga gatctgtgtg caaaaagatt
    40021 tttgcagcag tcggggtatc attgttttta tttcattcgc aaaggctttc cggacaaata
    40081 aaaggagata tagacacctt tgatacaaca aaaatagtgc tgcattctcc tcgaaaagct
    40141 tcattttatt ctgctgtttt gccgggtttg gggcaggtat ataataaaaa gtggtataag
    40201 gtgcctttta tctatgccgg gggggctgtg ataatttatt atcttaaatt taaccatgat
    40261 aagtatttag aataccgatc gaattatatc cgcaaagttg ccaatgaccc caatgatccg
    40321 gttgattata agtttaggta tgctagtaaa actacaatca aaaacaacat ggattattgg
    40381 cgtagaaacc gggactggtt aatagttggg cttagcgccc tttatattgc caatatactt
    40441 gatgcaacgg tagatgctta tctttttgaa tacgatatct ctcccgattt aagtatgaaa
    40501 atggaaccag tgttgctaaa tattggtaat tcacctactt ttggtgtaag tttgaatatg
    40561 cgattttaaa aaaactaaat tatgactgca aacctgagaa aagttttatt gatagtgttg
    40621 gtttttagtt gttttacaaa tattaaagca agacaacttg acgatactac tgatattgac
    40681 caggtaaacc aaaccaaagt ggtatttgaa gacaacctgg acagcttgtt gaacagttat
    40741 tatttgcaac aatcagatgt tgaaaacgac ctggctgttg atactgtaat tgaaaacgtt
    40801 gaatacagta acatccctga ttcggtctat gctgaacgtt tggcccgtat cccaagtgtt
    40861 attaagatga cttacaatcc aatagttcgg aaatatatag aaatgtattt acagcgaaaa
    40921 aaagaccgtg tacaggtgat gcttgggctt tctgagtatt atttcccaat atttgatgat
    40981 atttttgatt attacggatt gcccaatgaa ttgaaatata tgtctattat tgaatcggcg
    41041 ctaaattgcc gtgccaggtc aaggaccagg gcaattggcc tgtggcaatt tatgtatggt
    41101 acgggtaaac tgtatggact tactataaac agtttggttg acgaaagaag ggatcctatt
    41161 aaggaaactc atgccgctgc caaattttct aaagatttgt tcaatgtttt tggcgattgg
    41221 caattggtca ttgctgcata taactgtggc cctggcaatg ttaaaaaagc tatccgtcgt
    41281 tcgggcggga aacgcgactt ttgggaaata tacaggtttc ttccccgcga aacacgcgga
    41341 catgtaccag cttttattgc tgctacgtac gtaatgaatt attacaaaga acataattta
    41401 aaagctgtaa aaagtgattt tccgcgtgtt actgatacta tagtcattca tgatgacttg
    41461 catttgatgc aagtttcata cgttttgggg ataccaatag aacagctgcg ggatcttaac
    41521 ccgcaatata taagggatat aataccagca aaggggacat ctatggctat aactattccg
    41581 gcagatttta ccaataaatt tattgattat caggatagta tctttgcatt taaagattca
    41641 gtttttttca accctaagga gttaaacaaa aatcccaatt atgaagcccg ttattatggt
    41701 aaagctccta aaggctacgt aaaaataaga catgttgtaa aagaagggga gtctttgggt
    41761 gccatttctg caaaatataa gtgcagggta aatgatatta tggattggaa cggtttgtat
    41821 tcaagccgta tccgcgaggg gcgcatactt actatctatg tacctcaaaa attgtctaaa
    41881 caatatttaa gtgaaaatac aactgccgca aaagagaatg ctgcttctgg tctcataacc
    41941 tcagacaata caaaagctca aggcggacaa attgtatatt acacagttaa acaaggagat
    42001 acattgtggg aaatagctca aagatatcct gggatcagtt gcaatgatat attgagatgg
    42061 aacggattgt cccggggggc taatattgtt cccggacagc aactgaaaat aaaggttatg
    42121 tagtttatat tcaaaaaaaa gtagataaac tcggagattc tcagaaatag ttttacatta
    42181 gcaggcattt tagagcctaa atacaatgca aaagaactta gaaaaaggta aaattggcct
    42241 gtttacaact tctgcctttg tcattgccaa catggttgga acaggggtat ttacttctct
    42301 tggttttcag ctcaatggaa ctgaaaatat tatagctgtt ttacttctat ggtttttggg
    42361 agggataata gctctttgtg gttcgctcgt ttatagtgaa cttggtgctg caatgcccag
    42421 gtccggaggc gaataccatt atcttacgaa aatttatcat ccctgggttg gctttctttc
    42481 tggttgggta tcggttactg ttggttttgc agctccggta gcgttagcag caatggcttt
    42541 aggtaattat acgtcaaggg tgattcctgt gttagctcct gaaactattg ctatttctgt
    42601 attgttagta atcactttta ttcattcttt tgatgtaaaa gttggaggaa agtttcaaaa
    42661 tgtatttacc agttttaaaa tattgttaat agtgtttttt gttgtatgtg gttttttatt
    42721 ggtcccatcg tttcaaagct ttgccccatc tgtaaaaagt tttagtttgt ccgagctgct
    42781 taagcctgaa ttttttgtat cattgatata tgtaacctat gcattttcag gatggaacgc
    42841 ttctgcttat attgcttcag aaatagacca gccacaaaaa actattccaa aatcattaat
    42901 aataagcacg ttgattgtga cggtgttgta tttattgcta aacttagtat ttttaatgac
    42961 aacacctgta ggagagctaa aaggacaact tgaagttgga tatattgcag caaacaaaat
    43021 atttggcctg gttggtggca acctgatggc tgttttgatt tctatattgt tgatatcgac
    43081 cattagttct atgatatttg ttggccccag ggtaacgcag gttatgggag aagatattcg
    43141 tattttccgt tttctgggcg aaaaatcaaa aagaggcact cctgtttatg ctttaattct
    43201 gcaatttgca ataagttttt tcatgattct tacatcaact tttgaaagtg tacttacata
    43261 cgcaggtttt accttgaatt tatttacatt tcttacggtg atgggtgttt ttgtccatcg
    43321 aaagaaattc cccaatgcag aaagaccata caaaactctt tggtatccct gggtaccaat
    43381 tatatacatg tttgtaatat tattaactat tggatatctg gtatatatta ggccttacga
    43441 atcattaatg ggttttatta ctttattttc cgggtctgtg ttttatataa ttaaccaatg
    43501 gattgatact cctaagaaaa aagtcaaaga aaatcaataa tattttagtt atgaaattga
    43561 ataaagttgt tattgcaata atgatcccga tggctttatt agcaattatt tcttgcaata
    43621 atgccaatgg atctggcatt aataatgcaa aaaagcattc tgccgagtct gttaccctca
    43681 ataatattgc taattatatt gccggtttag aaaccgatac cagttgggag caaaaaagac
    43741 ttaccaagtt tgccgattgg caaaaatata aggcagaatc aaaaaatgcc tgggaaaagt
    43801 tttataaaga agcctcaaaa tattctgcat ttgctcaaaa agaaatacct cataaatacg
    43861 acacaataga aaccttattt tatcctttta gcgggcccga ttttcttttt gctaatacct
    43921 tatttccaaa ggttaagaat attatcctta ttgggttgga gaaaccaggg agcgtgccta
    43981 agcttcagaa ccttgatgaa aaaaatttgg cagacgtttt aaatttatac aaaaaagcaa
    44041 ttgaagatgt catgcaattg agtttcttca ggacacttga tatgaaagaa gaacttgcta
    44101 atagtaacat tgacggtaca actccagtgc ttatgttgtt tttggtaaga agtgaaaaga
    44161 gaataaataa agttgaatac atcaacctta atgaaaatgg taatttggtg gttgttgacc
    44221 ctgaaaaggt taaggattac attggagtta agatttatta cactgacaag gacaacgaag
    44281 tggaaaaaac aatattatat ctttctacta atctggctga tccttcactt tcaaaagaca
    44341 aagcattcat ggcatttttg aaaaacatag acaatccttg tataacactt gtaaaatctg
    44401 ctacctacct gatgcataaa tcgtactttt ctattattag aaatacatgc ttggagcatt
    44461 cggctttgat tgtccaggat gattcaggaa ttgcttataa attttttgcc cctgaaaagt
    44521 gggacctaag tttgtatggg tcatatacta agcctattaa attgtttgaa gaattctatg
    44581 aacctgaatt aacaaaggct tttgaaacaa aaaaagccaa accaatcaat tttaggtttg
    44641 ggtatagcca aaaatcttgt attttgattg cagaaagaaa ataataaatt tgggaggaag
    44701 gcatggggag aaaccttatc gtaacagcgc tgtcaatgtt tatttttatt aacattgaag
    44761 ctgaaagtat atctaaagca ttgcctggag ttgataccag ctatgtaaca tctgaagaca
    44821 gcttgtttaa acaagataca gtgtatgtag aagagcttca ggaaagtgat tttcaagatt
    44881 atcttgatac tgtgtcaatg gcggtagata ctgtcttttg ggacaataaa atgattaatt
    44941 cgggccgttt tgattcaaaa aatatgtgcg atacggttag ctttgtgtta agagattcga
    45001 caactgggaa aaacttttat attccattta aaaactatat taccagtaat tttgggcctc
    45061 gcaggtatat ctttcattat ggtgtagata ttaagctatt taaaggggat tcagttcatg
    45121 ctgccatgga tggggtagtc agggtcacaa agtatgatag gcgtggtttt ggtaatgtta
    45181 tagtgataag gcattcatct gggttggagt ctatatatgg gcatttgtca aaagtacttg
    45241 ttgagcccaa tcagaaggta aaagctggcg aggttattgg gctcggaggt aataccggcc
    45301 gttctacagg ttctcatttg catttagaat ttaggtataa aggcgaacct tttgacccca
    45361 attatgtgat tgattttgaa aaatcatgtt tgcgccgaga tacgcttgtt ttgacaagaa
    45421 ataattttga atatcttgta gaacttagaa aagcgaaata ttgcactgta cgaaaaggcg
    45481 ataatcttgg taaaatagcc cgaaggtatc atacaactgt tgccaaactt tgttctttga
    45541 accatattaa ccgaaaaacc attatcaggg ctggccgtaa gctgagatat caataatcag
    45601 ttgaaaatat gagccagtcc aacaatacag gtgcgccttt tttggtaaga tgggtgatac
    45661 taattattat tagtgttgcc atgatgggta attactatat atatgatagt attagcccat
    45721 tggctgatat gctaaaagtt cagttagggt tttcgagtac tgcaataggt acattaaacg
    45781 caatatacag catccccaat atttttatgg tacttattgg gggaattatt attgataaaa
    45841 ttggtgtgcg aaaagcaggg cttattttca ctgcattaat catgtttggg gctgtcatta
    45901 cctgcttaaa aggtagtttg atagtaatgg cgaccggccg tctgattttt gggcttgggg
    45961 ccgagtctat gattgttgcc attaccacct ctatagcccg ttggtttaaa ggaaaagaat
    46021 tttcgtttgc ttttggcata aaccttactg ttgcaaggct tggttctttt ctggctttaa
    46081 actcgcccaa ctgggcagca tcgttttatg ctaaaggttg gcagtttcct ttgtttattg
    46141 ctgctattgc cggtattgct gctgtaattt gtatggtggt gttttatttt gctgatggca
    46201 ttgcaggtaa aagataccat cttcctaaag aaggaaacca ggacaaaatt gagtggaaag
    46261 ccatttttaa gttttcaaca tctttttggt ttatcagtat gctttgtatc gttttttact
    46321 ctgccatgtt cccttttcag acttttgcag taatgttttt tgagcattca tacaacttag
    46381 ataatggtgc tggtggcttg ctttcgagta tgctgacttt ggcagccatg atatttacac
    46441 ccatctttgg atatttatca gatcttgtag ggcgtcgttc gttgtttatg attataggct
    46501 cattgatgat tatcccattg tacctgatgt tgggctacca tgttgattta gtttcggctt
    46561 tgcacctgtc ttctacatgg catgtaagta taaaaattct gagcatagat gcttttattt
    46621 cgcccaatgt gcttattcca atgtttgtaa tgggattggc gtttgctatg attccggcac
    46681 ttatgtggcc ttcggttgca ttggtggttg acaattcaaa gcttggaaca gcttatggcc
    46741 tgatgactat gatacagaat atcgggttgg ttggctttaa tctgttgatt ggcgttgtta
    46801 acgaccattt tcaggcagga gcaggcaatc cggatggcta cattccaggc atgtggttgt
    46861 tttcaatgtg tggagtgtta ggtattgtgt tttcctttct tttacgtaag agtgcaaaaa
    46921 ctgaaaaagg ggtcacgctt gatttaccaa tgagaaagaa ataaaatttt actcctttat
    46981 ttcgttaatt attttattta cctgttcttc tgtttgtctg agtttttctt tacagaaaag
    47041 taccagctca gatattcttt ttactttttc ggataattcg tcaatagaaa attggttatt
    47101 ttcaataccc tcaacaatca tttctatttc tgccatggca ttttcgtatg tcaaaccttt
    47161 tttactcata tttttttctg caaaagtatt aaaaaaagag gtgcaggtgt ttaatttgcg
    47221 taattttcgt aattttgcat caattataac caaaaaattt atatacatga aagttgcaat
    47281 cgttggagtt agtggcgctg tgggccagga attccttaaa gtgttggaag aacgtaactt
    47341 ccctgcagat gaattagtat tgtttggttc ggcccgtagt gccggcaatt catacaattt
    47401 tatgggtaaa agtatcactg ttaaggaact aaagcataat gatgatttta agggtattga
    47461 tatagcttta acatcagccg gagctagtac atcaaaagaa tttgcaactg atataaccaa
    47521 gtttggcgct gtaatgattg acaattcaag cgcttttcgt atggatgcag atgtaccatt
    47581 ggtggtacca gaagtaaatc ctatggattg taaaaatcgt cctcgtaata ttattgccaa
    47641 ccctaattgt tcaacaatca ttatggtagt tgcgttgaaa ccaattcagg atttgtcgcc
    47701 tattaaacgt attcatgtag ctacatacca ggctgccagt ggcgctggtg ctgctgctat
    47761 ggatgagttg tttgcgcaaa tgaagcaagt ggttaaccat gaaaaagtgc atgtcgaaaa
    47821 gtttaaatac caattggcta ctaaccttat tcctcagata gatgtattta ccgataacga
    47881 ttacaccaaa gaggaaatga agatgcacaa cgaaacacgc aagattatgc actctgacgt
    47941 atgtgtaagc gctacaacaa tcagggttcc tgtattacgt gcccactcag aagctgtttg
    48001 ggttgaaact gaaaaacctc ttgaagttgc tgctgttcgt gctgcatttg aaaaggcaga
    48061 aggtcttgtt gtacaggatg accctaaaaa tagcatttat cctatgcctt taatggtagc
    48121 cggagctgat cctgtgtatg taggccgttt gcgtaaagat attgccaatc ctaacggtat
    48181 taccttctgg tgcgttggag accaaatcaa gaaaggtgcg gcattgaatg ctgttcagat
    48241 tgccgaatac atgattaaaa acagactgct gtaaaaagtt gattataaat aaaaaaagct
    48301 tgcttcggca ggcttttttt atgttaattt caatataaaa atcaaattgt tctgccgaaa
    48361 ataaaatagt cgggtatttt tgtagcatta accaaaaact tgaatttttt aacatgaaaa
    48421 aactaaatga aattaagaaa ggtatatttt ttatacttgc aatgatggca atgatggata
    48481 tttcagctca aaacccggtt ggcaggcatg gttttttaaa ggtaaaaggc aataaaatag
    48541 taggcgagaa aggtgaagag gcccaattgc gcgggatgtc attgtgctgg acacaatggt
    48601 ttggcaaaca ttacaattac caaacagttc aatggctgca agaagactgg aaatgcgatg
    48661 tagtgcgtgc tgcaatggct gttgaacacg acgggtacct tgtacatcct gatatggagc
    48721 aaatgaaaat agaaaccgtt atcaatgctg ctattgatat tggtatgtat gttattatag
    48781 actggcacga ccatcatgcc cagcgcaatg ttgaggctgc taaacgcttt tttggcgaga
    48841 tggcgcgcaa gtatgcctta tttcctaata ttatatatga gctatacaat gaaccgctcc
    48901 agatttcatg gaaagattca attaaaccat attgtgaggc tgtgattgct gagattcgca
    48961 aacatgacaa acgcaacctg gttatttgcg gtactcctac ctggtcgcaa gatgtagaag
    49021 tggcagctgc tgaccctgtt aaagatgaga atgtggctta tgccatgcat ttttatgctg
    49081 ctactcacaa acagtttttg agggacaaaa ccaaaaaggc tttggatatg ggtgtggctg
    49141 tgtttgtatc ggagtttgga atatgcgatg ccagtggcag agaccctatt gactatgaag
    49201 aaaccaaaac ctggcttaag ttttgtgatg aaaacaagct aagctattgc aactggggta
    49261 tttacgataa aaaagaggct gcagctgctc tttatcccgg aaagaacacc tttggcggat
    49321 ggactttcga aatgttgacc ccgtctggaa aatttatcag ggcctggctg agaaaagaag
    49381 cattggtaac aaagtaaaag agacaagaac caagagacaa gaaatactta gtcaagataa
    49441 tattaaagcg tttcattgaa ggtaaaactg aaatggaacg cttttttagt tggccgaaaa
    49501 agagctaaaa cctttcaaag tttctcaaaa ttggaaaggt taagtcgtaa aaaaagcttt
    49561 ttataaattt tgagggttta ccaagctata tttgaacaga aagaattgaa acctgtccag
    49621 tttgaagtat tgatttcttc tgcgcaagct ttttttacaa tatctaaaaa tgcgcttctg
    49681 tctatcaatt cagaaccaaa gcgcatgaga tgttctgttt gcatttggtt gtctattatt
    49741 tggtaacctt tcacgtttaa atactttacc agatggcaaa aagccacttt tgaagcatct
    49801 gaaacatgat aaaacattga ttctccaaaa aacaccttcc caatagccag tccgtagagg
    49861 cctccaacta acttgtcatt ctgataagct tcaatagaat gggcaatacc cattttatgg
    49921 aggtttgcgt atgcatcgat tatttcattg gttatccagg tgccggtttg tttttttctt
    49981 gcagttttgg cacaatgcac aattacttcc aaaaatgcgc tgtcgcattt tatagtgaac
    50041 tttttctttt taagaaccgt aagcaggctt ttgctaacct ttaatttttc ggggaaaagt
    50101 acacatcttg gattgggcga ccaccacatg attgggtcgc cagggttgaa ccaggggaaa
    50161 attccgtttt tataagctaa aataatcctt tcggaagtta aatcacctcc ataagctaag
    50221 aacccatctt cttcggcata cgatggatga ggaaatatca aatctgcaca aagttcaaaa
    50281 acaggcatgc attaaaccag ggcaatttct ttgtgcataa atgtagaaag ctgtccatgc
    50341 tcatcttcca ggactaattc tcctgtgtcc aacacatctg taattttgcc ctcaaaatga
    50401 tggttcatgt cgctgtattt gagccacttt tgaaagccat agaggtgttc aaggtagatg
    50461 tttttgaatt gcgaaaaatc agttttatac atttgctcaa tccttgattt caggcagttt
    50521 aacagttgat gaagggcaat gtcgaggtta aaatacatac ctgttatttc cttcattgaa
    50581 atagctgtgc ctgaaagttg tttaaaatcg gtttggttaa tattcaggcc aataccaatg
    50641 accgaatgct gaatgtattc accaataatg gtattttcga ttaaaatacc ggcaattttt
    50701 tttctgccgt gcaaaatgtc attaggccat ttaatacggg atgatatttt gttttcattt
    50761 aaaaaatcta atattcctaa tgatgaagca atagtaatgg caaactggtt ttcggccttc
    50821 aaatcacgag gtttgattat taaagagaac gtaaggttgc aattggattc gctttcccaa
    50881 gtgttgccgg cctgaccctt accgtttgat tgtgtacggg ttgaaataac ctgttcggcc
    50941 agattttgat tttgttttat caaatcaagg gcataattgt tagtagagct gaccgtgtta
    51001 aggctaacta taggccataa atgttccata gtaattagtt tagtgttagt tacaaaccta
    51061 tattttttat ttttaatttg gttattttaa agcacaaaaa tttcgattaa aaaaatgtta
    51121 atttgttttt tgattctttt cgtttgaaaa caaggattac cttagcaaca cttttcacag
    51181 tcagacgacc ttaccttaac attatttaat tttgttcagc acatttttga tgtgcttata
    51241 tgtttttttt gatttttatg agtaaaagat ttcggtttgg gcttattggt ctattgcaaa
    51301 ttactatact aaatattttt gcgcaagata tagaacaaaa gcaggtttca gtatctactg
    51361 aaaaaacttc attaatccaa tttctaaata aagtaagtac ggaaaatcag gtacagtttt
    51421 attataaaaa ggaatggttc gaaggcgatt cgatagcctt gactatttca gatattcagc
    51481 ttggccgttt actcgataaa atttcagact cacgggcaat tatgtggttg ccaatatctg
    51541 aggatgccat agtttttttg cctaaagacc aggtagctac gttgcttggg aatatggttg
    51601 atatgagcgg ctcaaaaaac ataaatactg ttgaagttgg taaaatgtct gaagcaggaa
    51661 aatatgctaa tgtaatattg aaaggcacag ttaaagacgg caaaaatgac gaacctgtaa
    51721 taggcgccag tttgaggata gccaatacca atataggttg tatgactgat gccaatggaa
    51781 agtatcagtt gaggcttagc ccaggtatat atgaagttga agtgtccagt gtagggtatg
    51841 aaaaaaatgt ttggaaagta aaacttatca gcaccggaga gtttaaccct gaaatatttg
    51901 aaaaatctac taaaatagac gaaatcacta tttatgccaa acgggcagat aaaaatgtcc
    51961 gaagcaacca gatgagtttg attgatttgg attcaaagtc tattaaacag cttccttcga
    52021 tggtgggcgg aaaagatatt ataaaaagct tcacgatgat gcccggggta aaatctgttg
    52081 gcgaatttgg ttccggtatc aatgttcgtg gtggtggcga tgaccagaat ttatacctta
    52141 tagaaggggc cccgttattg aatacttcgc atgttttggg gatgatgtca gttttaaacc
    52201 ctgatattgt aagcaatgta gctttgtata aaggccaaat ccctgccaat tatggcgaaa
    52261 gggtttcttc tgtaatggaa atccaggtga gggaaaataa tattaaaaat tttcatgtca
    52321 aaggcggtat tgggctttat gatggtaggg ttttagtgga aggcccgatt tctaagaaag
    52381 taacttataa aataggaggc cgctcaagtt attccaattg gcttcttggt gttatccctg
    52441 atgccaatat tcaaaagagt gccgcttctt tttatgattt gaacagctat atctcatggg
    52501 ctggcgaaaa agaccgggtt tcgttatttg catattacag caatgattat ttcaggtatg
    52561 cccgtttaca ggcatacaat tatggcaaca ttctcggttc g
//
LOCUS       DAOSPF010000017        52516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_680_length_52516_cov_6.923905, whole genome shotgun sequence.
ACCESSION   DAOSPF010000017 DAOSPF010000000
VERSION     DAOSPF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_52516_cov_6.923905"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PK252_05120"
     CDS             <1..1594
                     /locus_tag="PK252_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT14125.1"
                     /translation="QTYYDSNGDGIGDLQGIIQKLDYIKSLGVDAIWINPFFESPFCD
                     AGYDISDYYKVAPRYGTNEDAKRLFDEAHKRGLKILFDFVASYTSMEHPWFKASARQD
                     TNKYTNWYIWSDNVWMNPPKEYLDAFIKGYGTRNGQYMRNFYYCQPALNYGFALPDTN
                     YKWQLTINHPDVLSLREEMKNVVRFWMNMGADGFRADMAGAMVKSANVDGNPQFFNTH
                     EDGTKKFWQEIRQILKKDYPEAFMVAEWSYPIAALDGCFHADFFHWFDGYNDLFQKES
                     WRILNGYSQGHSYFDIEGKGNITHFLEKYMEQYNATKGKGYISLPLGNHDNARLGNKR
                     SDDDLEIIYAFGLTMPGVPFIYYGNEIGMRQLPVTWPQVEGAYRPRNGARTPMQWNNN
                     KNLGFSTAEANKLYLPVDTATNAPNVEAQDKNPNSLLNRTRNLIKLKKSEPALANYAE
                     FIPLYAKENTYPFIYARAKDKDVVLVMLNPSGKEVTAEFDLNIKYAKTVVLAGKTLKL
                     KKNGQKVSVTMPGQSYSIVKLQ"
     gene            1741..2118
                     /locus_tag="PK252_05125"
     CDS             1741..2118
                     /locus_tag="PK252_05125"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14126.1"
                     /translation="MEPLRQEKTKSTPEINFDKSQEIFEIKGWSKPENTLEFYEPVLS
                     WLTEYSAQPNPNTVFNFNLDYFNSSTSKIILNIIRVLEAIPQNGQKLEINWYYNDEDN
                     LEAGEAYKSLTEVPFNCIKTSGN"
     gene            2229..3269
                     /locus_tag="PK252_05130"
     CDS             2229..3269
                     /locus_tag="PK252_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT14127.1"
                     /translation="MKSKYPVGKLIRFNFWLTAYFFLLGFVTTMAGVGLSGLHEAISR
                     ASFGALLLSLNAFTNLGLLLAFELDVIENKNKHKTRFLATSIILNSIIYLTVTILNSM
                     VFGHKLHTFTIIITVILSILVNMLILALQNFIVLHEAKANADIENSKLRAANADAANQ
                     LLRQQIHPHFLFNALNILKSLYRVNAKLGEEYLIRLSDFLRAAVSNNNIKIIPLKEEL
                     KLCQDYLEMQKIRFNNSLEYSVSISTEALNSGYVPSFSIQPLLENAIKHNELTEESPL
                     HIDILQEGNWVKIVNNLKRKNTSETSTGSGLMNLAERYRLLSGEELFIDETDKTFTVS
                     IKILKQGNSLQK"
     gene            3302..4060
                     /locus_tag="PK252_05135"
     CDS             3302..4060
                     /locus_tag="PK252_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT14128.1"
                     /translation="MKIVIIEDEKITADDLASTIMSIEPTAEIVAILSSVKNAVAYFQ
                     KAESPDLIFSDVQLGDGLSFEIFKAVNVSAPIIFCTAYDEYALNAFKTNSIDYILKPS
                     TTISISEALDKYRNLKQSFSNQERQYKTIIEALLHQATPKSNAILVHYKDKIMPVKTE
                     DIALFYIENEITYLLTFENKNYCINKNLEELEDICGSLFFRANRQCLVNRKAVIDASH
                     YFSRKLSVNISVPFQEKILVSKEKSPAFLKWLEL"
     gene            4254..4631
                     /locus_tag="PK252_05140"
     CDS             4254..4631
                     /locus_tag="PK252_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT14129.1"
                     /translation="MSNELPKKENILREKSYAFAIRIVKLAQHLNNEKHEHIISNQIL
                     RSGTAIGALVRESEFGQSKADFISKLSIALKEANETDYWLSLLKDSSYLSENEFNSIS
                     PECTELIKLLISSINTAKNNNKK"
     assembly_gap    4791..4890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5024..6430
                     /locus_tag="PK252_05145"
     CDS             5024..6430
                     /locus_tag="PK252_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT14130.1"
                     /translation="MEIRLKVLATFLFGAVLAGSFQASGQTKLNDIISYALEHSREVK
                     KANYQIEEAKYAKREAIGHGLPQVEASTSYSKMILPEIGISNEASQQIAGMANLLINE
                     SVTAEQRQQITNSISGMLNQLNNLDALYTASAGIQVTQLIYSQAYWVGLKTAKKAEEL
                     YSILKTKSEEDLIAEVATGYYQAGSLMLQLQTVDKSLNNLKEIHRMVELSYQNDMVKE
                     SQVNRLKVTISNLEVTKQTVQNGITIQLNYLKALAGMPEDTALTIDTTSLINDFINNG
                     HSFDFNAENVPDYQALIKQNEVYEQQVRLSKAKYYPTLAAFAKISYSSYNTEMEIKKF
                     SNTNTFGLSLTMPIFASGSDYAKVKQAKLKQAQLQQDILKTKDLLTVSYNNAFLEYQT
                     SKDMVTAQKENKDLAMKVYHQTSLQFQEGMASMADLLNVNSDFLQADNSYNQQILKCK
                     TAEIKMLKASGKLKNIVK"
     gene            6561..7622
                     /locus_tag="PK252_05150"
     CDS             6561..7622
                     /locus_tag="PK252_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT14131.1"
                     /translation="MKIRKSRLIIIAIVVVAIVFVAFKLAVNKQKFQEEVAFSQRTID
                     KIPVAVEVASKNVLSENVVATGTLEASDVLNLVSETQGKIIAIYKEKGDRVSVGEVIA
                     KVDDEVISANVLTAEANYTQFEKDVERLTRLAEQNAVTKRDLEQANIGLKKAKADLIN
                     ARKALNNTSIKAPISGYINNDFITVGQLLGGGSPVCEIVNNSQLKLNVKVSEREAYKI
                     KIGQNVAVRLSVFPDKKFSGRITSIAEKADAAMKFNVEITISNNGAHLRSGLYAEAEL
                     PVKNEENIIIKKVSIIGSMENPQVFVVEGEKAIRKTLIIGQSNNKEVEVLSGLTEGDK
                     VVVSGQLNLKDGDNVKVIN"
     gene            7681..10800
                     /locus_tag="PK252_05155"
     CDS             7681..10800
                     /locus_tag="PK252_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT14132.1"
                     /translation="MKLTELSIKRTTIPVVIFALLALGGLFTYSLLNKELFPNIDMPV
                     NAVLTVYPGAAPSEVESSVSEKIEDAISSLEGIDKITTYSFESMSVVVITYKDGIDAD
                     LSLQECERKVNAIKSNLPENIKDPQFMKFDLGMFPVMTIAANANIPEKEFYDFVDNDI
                     KTRISQVKGVASVSIIGGNQREIQIKVDANKIEQLGLSLVQVKQMIQAANVDFPTGKI
                     KDDDTKYIVRIAGKFDNLDEIRNLIVGATKDGSVIKISDIATVVDGSKKATKLARNNG
                     QPSIGMTIQKQTDGNAVEISKEVNKILADYEKEYTKDNLKFNVASDTSVFTDQAVDGV
                     MVDLVFAIILVSITMLLFLHTFRNLVFIFVSIPTSIISTFTFFWLFGFSLNLLTLLAM
                     SIVVGVIVDDAIVILENIYRHMEMGKSRWQATLDATKEIGLTVTSITIVLVAVFLPIG
                     LTTGITGQMLRSFALVIVISILISLLVSFTLVPLLTSRFGKLKEYNKTKFFDRFLLGF
                     EHVITWARLVILKGTQICLKHKAITIVTAVFCLLGSCSLLQFGFIQTEMMDAGDRGEF
                     IVTMELDRTATLEQTSSMCLKVEKTLMEHPEVTNVFTKVGSKGGSLSIAETPYAAEFN
                     VKLVDKNKRELSTKLFSKQLQNELKSKFAGPNFKITEISMMGSTTTPIEIYVRCNDFE
                     KAKAFSHIVTNELRSIKGTTDIESTIENGDKEMVVKFDREKLAILGLTIGEIGNQMYM
                     SFEGNRDLKYRDGSNEYDILISLDEFDRESKADLENIAFTNHAGQLIKLSQVADIIEA
                     ESPSTLNRYNKLPSVRITGNMIGKTIGTVGTELKERMAKIEKPAGVDIVYAGNMEQQS
                     KSFASMGIALIASLIFMYLIMVALYDSYVYPLVVMLSLPLAVIGALLALALSGKSLSL
                     FAIMGIIMLMGLVAKNAILVVDFANGAQEKGKKAFDAIIEATSVRFRPIVMTNLALII
                     GMLPIALGSGAGAEWKNGLGWVLIGGLTSSMLLSFIIVPVLYVTLDKWIKRFKKNNTP
                     EESSLIENP"
     gene            complement(11094..12212)
                     /locus_tag="PK252_05160"
     CDS             complement(11094..12212)
                     /locus_tag="PK252_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT14133.1"
                     /translation="MMNMKNLAVAAFALIVLVSCSEKKQTSLKDAYKDYFYIGAAIDR
                     DQIAGKDSAATQLLVSQFNSIVPENVMKWERIHPQMDSFVFAPVDSFVELGLKNKMFI
                     VGHCLCWHSQVPPYVFVDAKGKDVSKDTLLARLKSHITTVVSRYKGKVNGYDVVNEAL
                     NEDGTFRQSKWYQIAGEEYIEKAFQYAYEADSSAELYYNDYNIEQPAKRAGAIKLIKN
                     LQAKGIKVSGVGIQGHWHVNSPSMSVVDSAIVDFAKLGVKVMFTELDFTVIPNPWDNN
                     NANISDTFAFKKEMNPYADGLPDSVQQKLANRYADFFTVAAKHKDVVSRVTFWGLHDG
                     QSWLNNWPIFGRTNYPLPFDRKLQPKPAYYSILKVVSK"
     gene            complement(12287..13732)
                     /locus_tag="PK252_05165"
     CDS             complement(12287..13732)
                     /locus_tag="PK252_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT14134.1"
                     /translation="MDSKLSMKEKVGFSLGDGAANFIFQTMMLLQLSFYTDTFGLTAA
                     AAGWLFLVARLWGAIFDPIMGVVADRTHTRWGMYRPWVIWTAVPFGIFGFLAFTTPDL
                     GPGGKLFYAYVTYILLMTIYSMNNIPYSALSGVITGDMKERTSLSSVRFVVVTVATLA
                     IQGLTLPMVNHFGQGDSSRGYQVTMGIFCLLAVVFFFITFFTTKERIKPDPKQETSLK
                     QDIKDLLSNKPWVAMFVVFLLMFIFLAMRNSILLYFFKYYLDKDSMIAFLETLNKCLF
                     GLIAKVGMLGSNADISGNVFSIINIFGQLAALCGIIFSKPLAARFGKRDAFIFTIVLA
                     AVFAAMFIFLSPTAIWATFALQLLFNFSWGISMPLPWAMMADVADYSEWKNNRRATAI
                     VFAGIVIGLKVGLALGGALTGYLLSGYGYVANAVQTQEAISGIRLITSIFPAIALVGM
                     ITILFSYKIKKSVEYQMQDELAERRKSYSAE"
     gene            complement(14019..15098)
                     /gene="hypD"
                     /locus_tag="PK252_05170"
     CDS             complement(14019..15098)
                     /gene="hypD"
                     /locus_tag="PK252_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOT14135.1"
                     /translation="MKYISEYRDAEKVQQLAAYIKQIAKEPMVIMEVCGSHTMSIHRF
                     GLKHLLPEHIVLVSGPGCPVCVTPVDYIDKAVALARIPDVIIATYGDLVKVPGSSSSL
                     EKEKAKGADVRMVYSVLESIDFAVNNPDKKVVFLAIGFETTTPPTAAGILKAKELGLD
                     NFSVLSSHKIMPPPMQAIIEEGIAVDAFLAPGHVSVITGTEMYNFIPEKFGKGVVVSG
                     FEPIDVLQSVIMLIKQKNENRPSVEIQYTRVVKPEGNPKAQQMVNEIFEPADANWRGL
                     GIIPKSALSLREKYKSFDAEHVFQVIPEQSVEAKGCICGQVLKGLKKPSQCGLYGNKC
                     VPENPVGACMVSSEGACNVYYKYGE"
     gene            complement(15095..15328)
                     /locus_tag="PK252_05175"
     CDS             complement(15095..15328)
                     /locus_tag="PK252_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOT14136.1"
                     /translation="MCLSIPAKVEEINGNEAVVSLSGNKIRASLDLLDNIQVGEYVLV
                     HAGYAIQKVDEEEARQTLELIDILGMNDEIPQK"
     gene            complement(15406..17712)
                     /gene="hypF"
                     /locus_tag="PK252_05180"
     CDS             complement(15406..17712)
                     /gene="hypF"
                     /locus_tag="PK252_05180"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOT14137.1"
                     /translation="MQGNNTIVAKRFTVKGLIQGVGFRPFVFRLAAEYNLKGWVNNTN
                     EAVNIHVEGTSEAIHKFELNLEKKKPVHSEIETIKSSDVEIEHYEYFSIIQSTDVSKN
                     FTEVSPDIAVCEDCLDDMLQPSDRFHYPLINCMHCGPRFSIVKELPYDRCNTTMAPFT
                     MCENCRKEYEDPSDRRFHAQPIACNHCGPLYSLYKKNDETVVADSAENLQTIINQLQQ
                     GLGRGEIYAFKATGGYNLVCNALDEKAVARLRAVKHRDTKPFAVMFSSISTLKGYLYC
                     NHHEEQCLTSFRRPIVILKNKKELAHSVSKGFPTTGAMLPYMPLHYLIFQHTNLEALV
                     MTSANISEEPIVIDDEKALNAFLPLVDGVLTYNRDIHNRIDDSVLFVANDIPRLIRRS
                     KGYVPQSIDVVDNVEGIVAVGAELTGAFALGKGDKAILSQHLGDLQDFENFQFFVESF
                     ERFKHLFRFNADMVVCDMHPDYVTTGFAKSLNVKVVEVQHHHAHIASVLSEHKCTEPV
                     LGIAFDGTGYGLDGKIWGSEFLYCHKSEMERLAHFEYVSLPGGDMATLEPWRVAVSYL
                     YAAYGKDFKSLPLEMFKSIDAVKLNTVVTSIDKQINSPQSCSAGRLFDAVSALTGICI
                     LPSFHAEAPMRLEAAIVSDEKFYTPRVNGDEISFLPVIRQIVEELLSGTDKGIIAARF
                     HNTIINVVVEMAEKIRQKKEINIVAFSGGVFQNKYLLENSENKLSERGFKVLTNINVP
                     CNDVSVALGQLYLGLLYKNLKTTSTPSL"
     gene            17810..18514
                     /locus_tag="PK252_05185"
     CDS             17810..18514
                     /locus_tag="PK252_05185"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOT14138.1"
                     /translation="MNISEIELLNEKLKNKSPEEVIEHFIKLFGNRIALSSSLGAEDQ
                     VLTHMACNIDKNVRIFTLDTGRLFPETYELIDKTCKHYNTQIEIFFPDHQQVECMVKE
                     KGINLFYDSVENRKLCCNVRKIEPLKRAFAGLGAWICGLRKDQSVTRFYSKLVEWDEV
                     NKLVKINPLINWTEQQVWDYIRINNIPYNALHDKNFPSIGCQPCTRAIVTGEDIRAGR
                     WWWEQPEQRECGLHKK"
     gene            complement(18517..18750)
                     /locus_tag="PK252_05190"
     CDS             complement(18517..18750)
                     /locus_tag="PK252_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14139.1"
                     /translation="MYKSIVIHSRSTYWTNHEFSEEMEVDSDDLAKELDEKCNELEKQ
                     GFQIISILPVSSGNVVNGNGYYHTASIIITAHK"
     gene            complement(18834..20480)
                     /locus_tag="PK252_05195"
     CDS             complement(18834..20480)
                     /locus_tag="PK252_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT14140.1"
                     /translation="MRYCLFLIILFFVFPLKNSFAQRIPAERPKLIVGIVVDQLRYDY
                     LYRFMDKYEPNGLRRLMNEGLVCKNAHYDYMFSGANSGYATISTGANPSVHGIVSDEW
                     YVGLSDKTEHCSRDEKYNCVGNNESNEKHSASKLMSSTFGDELKLAWYKQPIVLSVSI
                     DPSASVMLSGHLVDGAYWFDVQTGTFVTSSAYKTTLPAWVTDFNKKKFADSYLDKTWT
                     TLLPDSTYVESLADNNSYERGIRGQITFPYDLKALSTIAGGKRDYNILRQTPMADNIV
                     KDFAISAIVNEKMGKDDIPDYLCINFCATGAIGKQFGIASREMEDACLRLDKEIKHLL
                     DFLDSYVGKGNVLVYFTAAHGATYPPKLMTDMGMPAGEFNLSLANTLLKSFLNATYGN
                     GNWIKKFSDHQIYLNESLIQDAKIDIAKFESDVAGFLIQFAGVANVATASMLQYSNYV
                     DGVLAKMQNSYYQKRSGNIVYQLQPGWAETTSNNSSSCTGYSYETHVPLIFYGWRISR
                     KVVYDKISIADIAPTLSLFLNIALPSGSEGKPAESILKLQ"
     gene            complement(20487..20861)
                     /gene="folB"
                     /locus_tag="PK252_05200"
     CDS             complement(20487..20861)
                     /gene="folB"
                     /locus_tag="PK252_05200"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT14141.1"
                     /translation="MSKAWFELEKMEFFAFHGCFSEERVIGNRYHVWLSYMTDISKAA
                     STDNIYDTVNYIDIYKTIQQEMAITSHLIEHVANRIANCLFEKFNAIDAVKLKLQKLN
                     PSMGGHMHAVSITIEKSRVDKK"
     gene            complement(20886..22619)
                     /gene="rho"
                     /locus_tag="PK252_05205"
     CDS             complement(20886..22619)
                     /gene="rho"
                     /locus_tag="PK252_05205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT14142.1"
                     /translation="MYDIIELNEKSLPELKEIAQKLSIKKTDNLSKQDLIYRILDQQA
                     IEVTNSKKKKEVSAVQGTSDGSEPVKNKRGRKKKSESEQGPAVAEEKIINEPVNKIEV
                     KVNPMPLQTETVTVPPVNQDEESKRIRPRVILAKKQPVKLDDVLDFPVEDIVDNSENI
                     EAVNSNVEGVDEPQITKAETEQAKEYRQEIQIKKNTDKRPEKLFDFEGIVTAVGVLEI
                     MPDGYGFLRSADYNYLNSPDDIYVSQSQIKLFGLKTGDTVRGTIRPPREGEKYFPLIK
                     VEEINGRDPDYIRDRVPFDYLTPLFPDEKFNLVSASKPNLSCRIVDMFSPIGKGQRGL
                     IVAQPKTGKTVLLKDIANAIAENHPEVYLIILLIDERPEEVTDMARSVNGEVIASTFD
                     EPAERHVKVANIVLEKAKRLVECGHDVVILLDSITRLARAFNTNAPASGKILSGGVDS
                     NALQKPKRFFGAARNIENGGSLTILATALTETGSKMDDVIFEEFKGTGNMELQLDRKL
                     SNKRIFPAVDLNASSTRREDLLLDKAMLNKMWVLRNYLSDMNPVEAMDFLIDRLSKTS
                     SNEEFLVTMNS"
     gene            22977..23840
                     /locus_tag="PK252_05210"
     CDS             22977..23840
                     /locus_tag="PK252_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOT14143.1"
                     /translation="MRQLKIVKQVTNRETPSLDKYLHDIGKVELITAEEEVELAKKIK
                     NGDEKALDKLVNANLRFVVSVAKQYQNQGLSLPDLINEGNVGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVRLPLNKIGSINKINKTFSKLEQEFEREPTILELSQ
                     ALELAPADIKDAMRTSGRHISMDAPIGQDDDGDMYDIYLNNDVPSPDRSLITDSLRHE
                     IERALSTLPDRDSDIIRYYFGLSGQSPLTLEEIGEKLNLTRERVRQIKEKAIKRLKTT
                     TRGSLLKTYLG"
     gene            23933..24589
                     /gene="rpe"
                     /locus_tag="PK252_05215"
     CDS             23933..24589
                     /gene="rpe"
                     /locus_tag="PK252_05215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT14144.1"
                     /translation="MSYILSPSILNSDFLRLSDSIEMLNNSEADWIHIDVMDGSFVPN
                     ISIGLPVVEAINSTAKKPLDVHLMIVNPDNYVERFYEAGAKILTVHYEACPHLHRTLQ
                     QIKAKGMKAGVAINPHTPVSVLEEIIPFTDMVLNMTVNPGFGGQKFIETSLKKIEKLK
                     SLITKMNAQTLIQVDGGVNSSNLKQLLNVGVDVIVAGSVIFQAPNPSATIKELKQISR
                     "
     gene            24611..25606
                     /locus_tag="PK252_05220"
     CDS             24611..25606
                     /locus_tag="PK252_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14145.1"
                     /translation="MRKVLFLLLILTAICGCKSKPTPPKVEKIPLTVLRFDKDLFAID
                     YATLEDSLPFFRKKYGEFFDIFNFKIIQIGSSENKGYADLLKGFVTDYTINKAKKECD
                     SVFADFNTIANNITSSMSYFHYYFPQKPVPTIITYVSGFNQSMVTTDTILGIALDRYL
                     GENHDFYKRMALPKYIRLKMNKSRIPADVVRAWLITNFPMNDSAFNLLSNIIYLGKIA
                     YASSLLQPNAEDTVFTGLSKDEIKWCKENEKNMWTFLVEKKKLFSSDMMLIRKYCEEA
                     PFTKDFGQESPGKAVIWIGYNIVNAYMANNPQVDLQRLMEMNDYMAILKSSKYKP"
     gene            complement(25652..25819)
                     /locus_tag="PK252_05225"
     CDS             complement(25652..25819)
                     /locus_tag="PK252_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT14146.1"
                     /translation="MAYKISNDCTACGTCISECPVEAISEGQIYKIDPEVCTDCGACA
                     DVCPVSAISQA"
     gene            complement(25898..26782)
                     /locus_tag="PK252_05230"
     CDS             complement(25898..26782)
                     /locus_tag="PK252_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT14147.1"
                     /translation="MATYSIKDMEKLSGIKAHTIRIWEKRYSLICPSRTNTNIRLYSD
                     AELRKLLNISILVRNGMRISQISRLPDSVLGEKVAILAQTTSKIEDLSEGLISAMIEF
                     DENKFDRILSNLIIKFGFEDTYIKVLTPFFEKVGILWQTGTILPAQEHFVSSLIRQKL
                     IIAIDSQRDVPITRKSNFLLFLPEGEMHELGLLFIQYLLRKRGYSTVYLGQNVPMDNF
                     PGLSEKLSVDVLVTSFTLSSVRVNAEKIVRNILLSFPHTKVILTGLQIKNFSFQKTHN
                     VHCYSHVHDFIQAIEVFD"
     gene            complement(26899..30024)
                     /locus_tag="PK252_05235"
     CDS             complement(26899..30024)
                     /locus_tag="PK252_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT14148.1"
                     /translation="MNESILKSLMRLFAIVADVKADGNFGNKRDIVIDYLDRQYSQEL
                     VDKYIQYFDDQVRLFHPEAMYANDDATAHQHNMNEEKIQEICEKINVELEMEQKMIVL
                     VYLLDFINRGEAITLKEHKFVFKVAIYLKINIDEFKDLRAFTFGEFAKIIHKDNILFI
                     DSNPTPEYADIKQMYNSKMEGRIAVIHLPITNTYVFRYDGNLTFYLNGHYVKPQRSYI
                     WVVGAVIKNPKVGSLYYTRMAGRFIQANIESKFVYTAEDIEFRYGNSTNGLRRFTLNE
                     ESGRFIGIIGGSGSGKSTLLNVLCGNIKPRSGSIKVNGYDIHQYKEELKGVIGFVPQD
                     DLLIKELTVYQNLYYNAKLCFNDYSEEEINRVVEHALVDFDLVEARDLNVGDAFTTIL
                     SGGQRKRLNLALELIREPSILFVDEPTSGLSSADSEKVINLLKKQTFKGNMIVANIHQ
                     PSSDVFKMFDKLLVVDQGGRIIYYGNPVDGITYFKRIAHFADAEESECLSCGNINPDQ
                     ILRTVEARVVDANGRLTRKRKTTPEEWYQLYLKNIDPEIRQIYREHDGSIPKNAFKIP
                     GHFKQFVIFLKRDVLAKIKNEQYLLLTAIEAPLLALILAFFTKRFVFVDGMAQYIFGE
                     NTNIPAFLFMAVIVSIFLGSIISAEEIFKDRNILKREKFLNLSRSSYLFSKVAIMFTI
                     SAIQMFLFVWIGNHVLHIEGMNWRYWVILFSASCWANMVGLNISAGLNSVVTIYILIP
                     LILVPQLLFSGVVVDFDKMHNKIASDMEVPVIGEFMVSRWAYEALAVTQFKDNPYEKH
                     FFQYEQLQKYANYYQSYVIPKLYELVDEAKAHKTNKADSSLVLNNMTVVCNEVKVMSL
                     LLGSSLPENIVPSTYPDDKQIAEIRLFLENANKRFLVWYQYSTSKRDSVVKALVKKTG
                     SVEEVLKLKQKYFNKQLAFVVTNEKELYDFSIQNGKILPIKNAVYRYPYSSNGRAHYY
                     APVKRVGKWLIDTFWFNVFILWFFSGLLMVVLYFDVLKRIIAYFETYRLNRLNRRRFM
                     RLLKVTDSFEK"
     gene            complement(30100..30927)
                     /locus_tag="PK252_05240"
     CDS             complement(30100..30927)
                     /locus_tag="PK252_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14149.1"
                     /translation="MTTKRNILNALGLVGVAIFISLASCQSSNNPQGTATSDSLNKNE
                     ITENVEAIVYPLPSPFEMTKMLNEIGAAYLVKNLNAPNKVEKYLTEKDKAINLGVYGA
                     DLAYASTYNKEQDIKIYLEPVKALIDDLGIKVDYSPLIDENLKAKLNNKDSLVKVVTN
                     TIYETYSFLNKQNKPELSAMLVSGMWIELMYIATHISKDTYNNTQIVDIVSKQSESYN
                     KLMELLGRYNNNKDINDIQQKLMVLKPAFDKVKSGLTEQDYSLILNTVEAVRKTFVM"
     gene            31224..32195
                     /locus_tag="PK252_05245"
     CDS             31224..32195
                     /locus_tag="PK252_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT14150.1"
                     /translation="MTHNNINITDILLSKYFSGNASEEEANAILMWKKASEANRKEFD
                     QSYQVWQLTMTAGISKLDPDAAWNKVNARAKVTKKPTFKVQRFYSNMLKVAAVLVVSL
                     LTWFTYDRYFSTVRYYADANIENVILEDGTNVTLNKNSWIEAPKHFRGSLRKVSMTGE
                     AFFNVKHDASHPFIIETKNAAIKVLGTSFNVKTMPEGNINVTVATGRVAFMPHNTKEA
                     VILSKGEYVSWNESKKELRKARSANQNYLAWKTHRFEFKENKLDSVIKAINAIYNSEI
                     ALGKEVSQLKLTARFDSLSIEQIVETLKVTFNLKAENTNTGLVLVKK"
     gene            32225..33775
                     /locus_tag="PK252_05250"
     CDS             32225..33775
                     /locus_tag="PK252_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT14151.1"
                     /translation="MSFRWVIISILFLQCSIQLTAQANRTINLCFENIEIAKAIDTIS
                     GKYHVNFSYNAELDVLKKKKKVCGEYTLSSALQALLNGENTGYSIISNTVVLFQLITP
                     DADSIQNISPPKPEFIDITGKISNATDKSPISYASVSVNKYPISTVCNNDGYFKLRLP
                     ERYYNDTIVISSIGYMQEKIAVSDFNGQIRNIKLFESVVNINPVIIKQISAENIVKES
                     LSRIKNNFCQRKAVYTSYYRETIKENEEYYSVAEGVLNIAKAPYNSHTQIDQVKIFKA
                     RKNENKLKLTSVIYKLEGGVANCVKMDIVKDEAPFYSSEAFNTYKYQVDRMDNYNGRL
                     LYIISFKPYSDTEDLTYTGKLYIDKESFALVAATFKLSPSGLERATQLLVKKTPRKTR
                     IKPYNTDYRVFYREINGKWFLDFTYTGIELKARSKIIGFNSTFSAISQMVVNNIDTTN
                     TERYKYRDISKVQDIFTEQIGEPDKNFWGECNIIEPEESLLEAIKRIRVESVQTKSQG
                     FWQKIFGL"
     gene            complement(33790..34191)
                     /locus_tag="PK252_05255"
     CDS             complement(33790..34191)
                     /locus_tag="PK252_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14152.1"
                     /translation="MFGLFERKKSHYQIDEEKKMVFIRFWGEICTNDIHKTFKALIEK
                     EQAVEGYHWICDVTKGKRLFETKQVDAFANVFKTHSEKFVNSKVAVVIANRKQSQTVD
                     ALVGYFDEHHIKIPVKKVLDTKEALHWLQNN"
     gene            complement(34543..36105)
                     /locus_tag="PK252_05260"
     CDS             complement(34543..36105)
                     /locus_tag="PK252_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT14153.1"
                     /translation="MRHFKNFMLFLMVAGVCCISGCKTKAGSTLEPIVKTTFGEVRGS
                     YSDTVYAFKGIPYAKAERFMPPQDPDAWDNVLECKNFGFVAKQIVPWIPDSTQDEKKL
                     FNLNVWTQGINDGKKRPVMVWLHGGGFHVGSSNDPMTYGQALAKKGDIVVVSVNHRLN
                     ILGFLDLSACGEKYAQSANVGMLDIVKALQWVKNNIENFGGNPSDVTIVGESGGGGKV
                     GTLMCMPAAKGLFHKAIIQSGTLINTMNKEKSQSLGLAVLENLGLKKEDVQKLDTISY
                     LALVKAGNDAIAKIAGPRKPGSPTMFGFAPSADGVVLIQQPFSPGFADFSKDIPLMMG
                     STFNELMPTAYADKKLTLEQAKQRLAKEYGNKTEQYIKLFAKAYPDYTPQDLLSIDKV
                     FRPYTIRTADARAKETSAPLYMYLLAWKSPVDSASKGAFHGLDIPLAFNNVDMRTDWT
                     STTPEAYKLADKMSSAWINFIKTANPNVEGMLPQWEPYTAQNGATMYFDNECKIVNNH
                     DRELMQFIKPLE"
     gene            complement(36275..37039)
                     /locus_tag="PK252_05265"
     CDS             complement(36275..37039)
                     /locus_tag="PK252_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HOT14154.1"
                     /translation="MDYFLTIAASDNSGGAGIQQDIKVAYNLGYWPLSAITGITVQNF
                     NKVFKVEAVQTSLLKFQIEQCFLSFPVKTVKIGAICSHDNLLAIVDCLKQFSQKHVIL
                     DPVLFSTGGVPFLDKSSLKVLQQALFPLTDLITPNKLEFEILTNQKINKIEEAIEIAI
                     EKCKEWNTSVLLKGGHFSDKLLKEALITPTDVYHFQRKRKKFSYSHGTGCTLSTALAC
                     YIGKNKSLYDSYLLSSKYLIGFYDKLHNPQVMDETN"
     gene            complement(37040..38314)
                     /gene="thiC"
                     /locus_tag="PK252_05270"
     CDS             complement(37040..38314)
                     /gene="thiC"
                     /locus_tag="PK252_05270"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOT14155.1"
                     /translation="MSTQIQKAKEGIITPEMLEVANKENVDVNWLRDEIAFGRIVIPK
                     NKNHTFPVRGIGKGLSTKINANIGTSEKHCNISEEMEKMKVAIKYGADAIMDLSTGGN
                     LHKILQQIISESSVMVGTVPVYGVVARLLSEGKRIAQLEPEDLFNEIEIQAQMGVDFM
                     TLHCGVTRHSLSFLDNDKRVCGIVSRGGALLKRWILDNKKENPLYEQYDRILEICKKY
                     DVTISLGDGLRPGCGADATDRGQISELLILGELVDRAREMGVQVMVEGPGHMPLDQIE
                     INMRLMKKLCHNAPFYVLGPLVTDSAPGYDHIVGAIGGTIAAIYGADFLCYVTPAEHL
                     CLPNISDVKEGVMAGKIAAHSADIVKNIYGSRQRDYVVSKARRELDWDTIFINALDPE
                     MAQKRKLESESSNEDYCTMCGSLCAVKNDKNI"
     gene            complement(38311..38925)
                     /gene="thiE"
                     /locus_tag="PK252_05275"
     CDS             complement(38311..38925)
                     /gene="thiE"
                     /locus_tag="PK252_05275"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT14156.1"
                     /translation="MENFGIYIIITKPYLSYSAIAEKCVAHGIQMLQLREKHLSDKEL
                     IKIAKNIRSITKGTSTNFVVNDRPDIAAICDADYLHLGQDDISVEDARKIVGNMKIGL
                     STHSIEQAKEALKKKPDYIGFGPIYPTNAKAKPDKPVGTEQLKQVLGFSHVPVVAIGG
                     IFPENIEQVIDAGAQNIAMVRHFMQTDEFDKRIQDLNNLLKRKR"
     gene            complement(38927..39694)
                     /locus_tag="PK252_05280"
     CDS             complement(38927..39694)
                     /locus_tag="PK252_05280"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOT14157.1"
                     /translation="MEQIVSKGIVDSYFEKLKENLSVDVAIVGGGPSGLVASYYLAQK
                     GLKVALYERKLAPGGGMWGGAMMFNEIVVQKEALFILDEFHITYKHFEKDYYTLDSVH
                     ATSALIYHATKSGVTIFNCTSIEDVVFQDNKVCGIVLNWSPVHREHMHVDPLFIMAKA
                     VIDGTGHDCDIARTLERKNNIKLNTATGKVMGERSLSVDEAERTTVENTKEIFPGLYV
                     SGMASNGVSGGFRMGPIFGGMLLSGKKVADLIAEKLK"
     regulatory      complement(39756..39847)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(39960..40952)
                     /locus_tag="PK252_05285"
     CDS             complement(39960..40952)
                     /locus_tag="PK252_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14158.1"
                     /translation="MCRRWLILGCLLVLSFTSKAGDTRIASLRGSWKFALGFKNEWIK
                     PEYNDADWDEVFVPKNWEDQGFYGYDGYACYRKKVVIKDFPEDRDLILSLGRIDDCDE
                     VYFNGKLVGFSGTFPPDFSTAYNVYRKYVIPSEYVNIGRYNTIVVKVYDGEREGGIVD
                     GDIALFLGEQKPPFEINLSGMWQFKVGDKADYKKPTFDDSRWNKLYVPKYWEEQGYKD
                     YDGFAWYRKTFVASEGLANDRVVIVLGKIDDQDEVYLNGTYIGTPKTLISMDAGTRYN
                     QLRVYYIDGSLLIPNKENTLAVRVYDSGGGGGIYEGPVGIMKQKAFVQFWRNKK"
     gene            complement(40973..42040)
                     /locus_tag="PK252_05290"
     CDS             complement(40973..42040)
                     /locus_tag="PK252_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOT14159.1"
                     /translation="MKTIVKILFLLMLTGCSITNIPASSNLRTLVNLSGTWLFSLGDE
                     KDWSAVKFDDSKWDQIKVPSSWENQGYVEYDGYAWYRKSFSLPQGASKYDICLQIGNI
                     DDVDKIFINGHLIGQTGKFAPNYTSGYGNERIYRIPANILNYNSENIIAVRVYDDQRD
                     GGITSGPVRLVYNEDEENLDYKLDGEWKFSIKDRTEYADINYDDSSWKTIKVPQSWQS
                     QGYWGFYGYAWYRYKFEIDPGMDLDNKYLVLGKIDDYDQVYVNGKLIGQNWSKTLFGN
                     TYFHNHDLYKILRGYKIPKNLLKPGINVIAVRVFDHGGVGGIYEGPIGITNKNSWEDM
                     RRKNENQIKGVENFYFGNFFE"
     gene            complement(42184..42543)
                     /locus_tag="PK252_05295"
     CDS             complement(42184..42543)
                     /locus_tag="PK252_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14160.1"
                     /translation="MKTREIFLVSVMFLLTLGVSVKATEAVTSNSVENSISNEITRKV
                     SNFIEDKLEMTREMDNTFALVYFQVDTKGKISFTNVDYEDRDMARSLELVVDRLDIEP
                     TTLKQGKYYIKVWFKVR"
     gene            42716..44260
                     /locus_tag="PK252_05300"
     CDS             42716..44260
                     /locus_tag="PK252_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT14161.1"
                     /translation="MFKKYTYIILFNALLLPAVLFAIFEFSALSSNEKVIQQVYNNQL
                     ETIVFSVNQYSDDIISSWAEKINYFLTEYNNPADTLRKYLKSKVQLSNVFLWKNNKVL
                     SLDKNIQPPKIVYIERLIKINDKQIRKNKSYLNVNYRKNEPLGTDEAQKKLYLAFWSG
                     VNPESAQLCVMEIDISKFVKEVIAVRLQVSVEKEFYVAVFNRAANNDLVYFSEYNKTP
                     PAFNFRKNLWLFPNYDIAVQIKKNTIEDVVKSRLLLNISLFAFIELLFVLVAVLIFRF
                     TRKEMKLAKLKSEFISNVSHEIRTPLALINMYIETLDMGRIKPEEKVKEYYQVILQET
                     QRLSGIVNNILNFSKLESGKTTFNFEINPVNCLVDEVLSTYNFHLKNKGFVLKTELAE
                     DILKVKVDKRSICDVLVNLLDNAVKYSAEVKEITIRTGVKSGWVYIEVADKGIGISKE
                     NQRFIFDQFYRVTQGNLAHHAKGTGLGLSIVKYVVEAHNGKIEVDSTEGKGSIFRVLL
                     PIKYDV"
     gene            44279..44974
                     /locus_tag="PK252_05305"
     CDS             44279..44974
                     /locus_tag="PK252_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14162.1"
                     /translation="MHKILVVEDEQAMLLGLRDNLEIEGYEVDTANNGADGLNKILEN
                     EYNLIILDVMLPQMSGFDVCRTVRKRGKKVPIILLTAKGEEIDKVFGLEIGADDYITK
                     PFSLRELLARIKAILRRTATIESDVEQGKLPQKIGRLQVNFTSYVATDETGAEVKLSH
                     KEFELLHYFVKNSGQVISRDELIKNVWNYNYEMTTRTIDNFVVKLRQKIEVNPNEPHL
                     ILTVHGTGYKMVL"
     gene            45196..45462
                     /locus_tag="PK252_05310"
     CDS             45196..45462
                     /locus_tag="PK252_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOT14163.1"
                     /translation="MKKEIHPKTYRLVAFKDMSNDYTFICRSAAASKESITIEGVEYP
                     LIKLEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKDHFDKKKGK"
     gene            45548..46732
                     /locus_tag="PK252_05315"
     CDS             45548..46732
                     /locus_tag="PK252_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOT14164.1"
                     /translation="MKNIILFDDCRVQLLPLTYTRPVAELRVGITTISEKWQYFLGDK
                     VSFYTEKYLSEKFPLCIEEDNYLINGSIIPDAALVSAIEKLQTADVLVKANTIIACRM
                     NKAATASFNNQISEAGKTIQYSGEYLQVKNTWDIFELNDKLLRIDFQTLTSGRNSQPI
                     SKTNNVISPENIFIEEGAKVECSILNASTGPIYIGKEAEIMEGCMVRGPLALCDHSVL
                     KMGAKIYGATTIGPGSKVGGEVHNSVFFANSNKAHDGFLGNSVLGEWCNLGADTNNSN
                     LKNNYAEVKLWNYATERFVNTGLQFCGLIMGDHSKCAINTMFNTGTVVGVSANIFGAG
                     FPRNFIPSFTWGGAHGLDTYPLNKAIETAQKVFGRRKKDFSEIEQNIFKQIFIQSEKY
                     RR"
     gene            46938..49355
                     /gene="lon"
                     /locus_tag="PK252_05320"
     CDS             46938..49355
                     /gene="lon"
                     /locus_tag="PK252_05320"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT14165.1"
                     /translation="MEFFPILPEDEENNRGEPSMPELLPVLPLRNTVLFPGVIIPITV
                     GRQKSMKLLKDAYKANIIIGAISQKDSSIDEPTEKDLHPMGTLAFVIKILEMPNGDTT
                     AIIQGRNPFRLGEVLVKKPYFKATYQVVDDIIPPAKDKEFNAILANVRELAIKMINYS
                     ANIPQEIGFAIKNIENPSFLIHFVCSNINISANDKQRMLEIPDLKQRAMLLLEFLNKE
                     VQALELKNDIQSKVRQELDQQQREYFLQQQIKTIQDELGGNPLEQEIEELRKKASKKK
                     WSKTVAEAFEKEIGKLQRLNPAAGEYSLQVNYLQTLVELPWNVYSTDVFDLPKAQKIL
                     DADHFGLEKVKERIIEHLAVLKLKGDLKAPILCLYGPPGVGKTSLGKSIAKALKRKYV
                     RISLGGLHDEAEIRGHRKTYIGAMPGRILQNIKKAKTSNPVFVLDEIDKVGNDFRGDP
                     SAALLEVLDPEQNNNFYDNYLELEYDLSKVMFIATANNISAIHPALRDRMEMIEVSGY
                     ITEEKVEIAKKHLIPRQLEAHGMKKASIAIKSEAIEFIIENYTRESGVRDLDQKLAKV
                     FRFVARQIAMEEPFNKTLGIEEVKKILGQPLFIRDKYENNDYAGVVTGLAWTSAGGEI
                     LFIETSLSRGQEKKLTITGNLGDVMKESAIIALEYIKAHADVLNLDMEIFNNWNVHIH
                     VPEGAIPKDGPSAGITMATSLASAFTQRKVKPNLAMTGEITLRGKVLPVGGIKEKILA
                     AKRAGITDIILSSENRKDIEEINEVYLKGLNFHFVKDIIEVYKLALLDEKVSNPLNLI
                     IEKPKKK"
     gene            complement(49647..51539)
                     /locus_tag="PK252_05325"
     CDS             complement(49647..51539)
                     /locus_tag="PK252_05325"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOT14166.1"
                     /translation="MKFLNSLKIGVRISVLISSAVVVILTMLGFYIYQLQSSKIIEDT
                     DIRMTEQLDDLCQIVQLQLKERQMRIESAMKVAHEILKNQGELSFDEKQKIEANVTGQ
                     TSQSTSNITIPALILNKKPIYKNFDLVDKITYLTDAKATIFQKIENGYLRISTSVLKA
                     NGDRAINTYIPNNSPVVLAIEKKEDYNGRAWVVDDWYFTSYKPLIVNNKVAGMLFVGI
                     PEKDFKNIKSIFDQKKYLQTGYPFIIDKDGKLIVHPSKEGAILSSEDFFKKMLASDSG
                     TGKISYTWEGKDKVLYFKYIKEIESYVAVSFYEDEMMKILYHLRYIIVLAILLSIGVI
                     VSINIYLSRNISNSINKGVNFARQIAKGNLTATLDIDQKDEIGVLASSLSQMAEKLRE
                     LLGGIDRGASEILMASQQISEGSQLLSQGANTQAASAEEVSSSMEQMSANIMQNKDNA
                     FQTEKISLHAQKGMEQMKNAGEKSIVSIKDIAGKITIINDIAFQTNILALNAAVEAAR
                     AGEHGKGFAVVAAEVRKLAEKSKAAADEIAQISQSSIGVTEESERIILSLTPEIEKTA
                     KLVQEIASASNEQSLGVEQINNALAALNHIIQQNAASSEELATSAEELASQAAQLKEM
                     ISFFKY"
     gene            complement(52005..>52516)
                     /locus_tag="PK252_05330"
     CDS             complement(52005..>52516)
                     /locus_tag="PK252_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT14167.1"
                     /translation="LRKNILISAKLLKNITDPNEDYIHIKLEPAQKKQLNASIADMKS
                     FTEYAPWWVILLISLSLGIGTMVGWKRIVVTIGEKIGKEHLSYAQGASAELVAASTIF
                     FSTSLKLPVSTTHILSSGVAGSMVATNGLKNLRKKTLKNILIAWLVTLPVTIILSGGL
                     FLLISYFMG"
BASE COUNT        16972 a        10417 c         9234 g        15793 t
ORIGIN      
        1 tcaaacatat tacgactcta atggtgatgg cataggcgac ttgcaaggta tcatccaaaa
       61 gcttgattat atcaaaagtt tgggagttga cgcaatatgg atcaacccat ttttcgaatc
      121 cccattttgt gatgcaggct atgacatatc tgactattac aaagtagccc cccgatatgg
      181 caccaacgaa gatgccaaac gccttttcga cgaagcccac aaaagagggc tgaagatcct
      241 tttcgatttc gtggccagct atacctctat ggaacaccca tggtttaaag cctctgccag
      301 acaagacacc aacaaatata ccaattggta catctggtca gacaacgtat ggatgaaccc
      361 tcctaaagaa tacctcgatg cctttataaa aggctatggt acccgcaatg gacaatacat
      421 gcgcaacttc tattattgcc aacctgcgct gaactacggt tttgccttgc ccgacaccaa
      481 ttacaaatgg caacttacca tcaatcaccc cgatgtactg agcttacgcg aagaaatgaa
      541 aaatgtagta cgtttttgga tgaacatggg cgctgacggg ttcagggccg acatggcagg
      601 ggctatggta aaatctgcca atgttgacgg aaatccgcag tttttcaaca ctcacgaaga
      661 tggcacaaaa aagttttggc aagaaatacg ccaaatcttg aaaaaagatt accctgaagc
      721 ctttatggta gccgaatggt cataccctat tgcagctctc gacggttgct tccatgccga
      781 ctttttccac tggtttgatg gctataacga ccttttccag aaagaaagct ggcgtatcct
      841 caacggatat tcgcaaggac acagctactt tgacattgaa ggcaaaggca acatcaccca
      901 ttttctcgaa aaatacatgg agcaatacaa tgccaccaaa ggcaaaggat atatcagcct
      961 gccacttggc aaccacgaca atgcacgcct tggcaacaaa cgtagcgatg acgacctcga
     1021 aattatttat gcctttgggc tcactatgcc cggagtaccc tttatctatt atggcaacga
     1081 aataggtatg cgccaacttc ccgttacctg gccacaagta gaaggtgcat atcggccacg
     1141 gaacggagct cgtaccccaa tgcaatggaa taacaacaaa aacctcggct tttctacagc
     1201 cgaagccaat aagttatatc tgcccgtgga caccgcaaca aatgccccaa atgttgaagc
     1261 tcaggataaa aacccaaatt cattattgaa ccgtacgcgt aacctgataa aactgaagaa
     1321 gtcagaacca gcacttgcca attatgccga atttatccct ttgtacgcta aagaaaacac
     1381 ctatccgttt atctatgcaa gagctaaaga caaagacgta gttttggtaa tgctcaaccc
     1441 ctctggcaaa gaggttacag ctgagtttga cctcaacata aaatatgcaa aaactgttgt
     1501 tttggctggc aaaactctta agctcaagaa aaacggacaa aaagtgtctg taactatgcc
     1561 cggacaaagc tattctattg taaaactgca ataaactttt tagataattg aaaaggtggt
     1621 taaacaattg tgcaaccacc tttttcattt aaaatcaaca atttatattt tctttcttga
     1681 ttcttgactc taaaaatctc ttaaaaaaaa tatctttgta taaacataaa aactcaaact
     1741 atggaacctt tacgacaaga aaaaaccaaa tcgacacctg aaatcaattt tgataaatcg
     1801 caggaaatat ttgaaatcaa aggatggtca aagcctgaaa atacacttga gttttacgaa
     1861 cctgtattaa gctggttgac agaatattct gctcaaccca atccgaatac agttttcaac
     1921 tttaatctgg attatttcaa tagttccact tctaaaatta tccttaatat tatccgtgtc
     1981 ctggaagcta ttccccagaa cggccaaaag cttgaaataa actggtatta taacgatgaa
     2041 gacaacctcg aagctggcga agcttacaaa agcctgacgg aagtaccatt caattgtatt
     2101 aaaacatctg gtaactaatc aatttttatt aactttcaaa taaattcacc ctttcttttg
     2161 tccgattcgg atacaaaaaa catttttgtg gccgcacttc atataatttt gtaatacatt
     2221 tatattttat gaagtcgaag tatccagtcg gtaaattgat cagattcaac ttttggctca
     2281 cagcttattt ctttttgctg ggatttgtca ctacaatggc cggcgttgga ctgagcggcc
     2341 tgcacgaagc tatttcaagg gcttcgttcg gggcattgct tttatctcta aatgccttta
     2401 caaacctggg cttgttatta gcctttgagc tggatgttat tgaaaataag aataaacaca
     2461 aaacaaggtt tctggcaacc agtattattc ttaactctat tatatacctg acagtaacta
     2521 tattaaattc aatggtattt ggccataagc tacatacatt taccattatc ataacggtta
     2581 tcttaagtat attggtcaac atgcttatcc ttgctttgca aaactttatt gtactacacg
     2641 aagcaaaggc caatgccgat atcgaaaatt ccaaactcag ggcagccaat gctgatgccg
     2701 ccaaccagct tctgcgacag cagatacacc ctcacttttt gttcaatgcc cttaatatcc
     2761 tcaagtcgct ttaccgggtg aatgccaagc ttggcgaaga atatttaatc cgtctgtcgg
     2821 actttttgcg ggctgcagtt tcaaacaaca acataaaaat tatacctttg aaagaagaac
     2881 ttaagctttg ccaagattat cttgagatgc aaaaaataag gttcaataat tcactggaat
     2941 attcagtttc aatttcaaca gaagcattga acagcggcta tgtaccttca ttttcaatac
     3001 aacccttact ggaaaatgcc ataaaacaca acgaacttac cgaagaatca cctttgcaca
     3061 ttgacattct gcaagaaggc aattgggtaa aaattgtaaa taacctgaaa cgcaaaaaca
     3121 ccagcgaaac ctctacaggc agtggattga tgaaccttgc cgaacgatat aggcttcttt
     3181 cgggcgaaga actttttatt gacgaaactg acaagacttt tacggtaagt attaaaattt
     3241 taaaacaggg aaattccctg cagaaatagc tatttcagtc aaacagaaaa accaacgacc
     3301 aatgaagata gttattatag aagatgaaaa aattactgct gacgaccttg ccagcaccat
     3361 catgtctata gagcctacag ccgagattgt agccatatta agctctgtaa aaaatgctgt
     3421 tgcctatttt caaaaggccg aaagtcccga tttgattttt agcgacgtac aactgggtga
     3481 tggcctgagt tttgaaattt tcaaagctgt aaatgtttct gcacccatca ttttctgcac
     3541 agcttatgac gaatatgctt tgaacgcctt taaaaccaat agtattgact atattttaaa
     3601 accttcgaca accatctcca tatctgaagc cctcgataaa taccgaaacc tgaagcaatc
     3661 tttctcaaac caggaaaggc agtataaaac tattatagaa gcccttttgc accaggcaac
     3721 gcccaaatct aatgccatac tggtacatta caaagacaag ataatgccgg tcaagacaga
     3781 agatatagcg ctgttttata tcgaaaatga aattacttat ctccttactt tcgaaaacaa
     3841 aaactattgt atcaacaaaa atctggaaga gctcgaagat atttgtggca gcctgttttt
     3901 tagggcaaac cggcaatgtc ttgtcaaccg aaaggctgtc attgacgctt ctcattattt
     3961 ttctcggaaa ctatccgtca acatttctgt accttttcag gaaaaaatcc tggtgagcaa
     4021 agaaaaatcg ccagcttttc tgaaatggct ggaactgtaa acaattaaca atgaatagtg
     4081 aacaatgaat aataaaatta aatactttga tgtattaatt ttattgatgt acataacgta
     4141 attcgttttt aattttgaaa caaaccctat tcttagattc aataaacttg attttttatt
     4201 gcactttaat acttatattt gaattattct ggacaattta ataaaagtca aatatgagta
     4261 atgaacttcc taaaaaagaa aatatactga gggagaaaag ttatgctttt gccatacgaa
     4321 tagtaaaatt agcacaacac ttaaacaacg aaaagcatga acacattatt agcaaccaaa
     4381 tccttcgaag tggaactgct ataggagctt tagtgcgaga aagcgagttt ggccaaagta
     4441 aagccgattt tatcagtaaa ttaagcattg ctcttaaaga agccaacgaa actgactatt
     4501 ggctttcgtt attaaaagat tcatcatacc tatcagaaaa cgaattcaac agcatttctc
     4561 ctgaatgcac agaacttata aaactgctaa tatcctccat caacaccgca aaaaacaaca
     4621 ataaaaaatg aacagagaac attgaataat aatgtgacgt aaccactaat tgtaaggagt
     4681 tgagcagtat ttaacaaatt ccataaagaa ctttaataaa gcaaattatt cacttttcat
     4741 tattcacttt tcattattca cttttcatta ttcacttttc attattcatt nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttcattattc atttatttca ccttcccttt
     4921 tgtccgtttc ggtatctaat ccattttacc tcatttaata acaacggaac tttgcttcgt
     4981 aattggaaaa atagtccaaa cgaaatttta agaagcaaag aatatggaaa tcaggttaaa
     5041 agtattagca acatttcttt tcggggcagt attggcagga agctttcagg cttcgggaca
     5101 aaccaaactg aacgacatca tcagctatgc actcgaacat agccgcgaag taaaaaaagc
     5161 caactatcaa atagaagagg ctaaatatgc aaaacgagaa gctataggac atggattgcc
     5221 acaagtagaa gcatctacca gttatagcaa gatgatactg cccgaaatag gcatttcgaa
     5281 tgaagcaagc cagcaaattg caggaatggc aaatctttta attaacgaga gtgttacagc
     5341 agagcaaaga caacagatta caaacagcat atcaggcatg ctcaaccagc tcaacaacct
     5401 cgatgcttta tataccgcca gtgccggtat tcaggtaaca cagcttatct atagccaggc
     5461 atactgggta ggcctaaaaa cagcaaaaaa agccgaagaa ctttattcga ttttgaaaac
     5521 aaaaagcgag gaagatttaa ttgctgaagt ggcaacaggt tattatcagg caggttcgct
     5581 aatgttacaa ttgcaaacag tagataaatc gctcaacaac ctcaaagaaa ttcaccgtat
     5641 ggtagaattg agctatcaaa acgatatggt taaagaaagc caggtgaacc gccttaaggt
     5701 tactatttca aaccttgaag tgacaaagca aaccgtccaa aatggaataa caatacagct
     5761 aaactacctg aaagctttag ccggtatgcc tgaagatact gcattgacta tagatacaac
     5821 aagcttaatc aatgatttta tcaataatgg acatagtttt gattttaatg ctgagaatgt
     5881 ccctgattat caagccttga taaaacaaaa cgaagtatat gaacaacaag tcaggctttc
     5941 aaaagcaaaa tattatccaa cccttgctgc ttttgcaaaa attagctatt cctcgtataa
     6001 taccgagatg gaaattaaaa agttttcaaa cacaaacaca tttggtctta gcctgaccat
     6061 gccaatcttt gcttcaggtt cagattatgc caaagtaaaa caggcaaaat tgaagcaagc
     6121 ccagttgcag caagatattt tgaaaactaa agatttgctg acagtcagtt acaacaatgc
     6181 ctttcttgaa tatcagactt caaaggacat ggtaactgct caaaaagaaa ataaggacct
     6241 tgccatgaaa gtatatcacc aaacatcact gcaattccag gaaggaatgg cctctatggc
     6301 tgatttgtta aatgtaaatt cggacttcct gcaagccgac aactcttata accaacaaat
     6361 tttgaagtgc aaaacagccg aaatcaagat gctaaaagct tccggaaaat tgaaaaatat
     6421 agttaaatga gagcaaagaa caaagaacaa agaacaaaga acaaagagca aagaacaaag
     6481 agcaaagaac aaagaacaaa gagtaaagaa caaagagcaa agaacaaaga gtaaagaaca
     6541 aagaataaaa aataatagtc atgaaaatta gaaaatcacg tctcataatt attgctatag
     6601 tagttgtagc aattgtattt gtagccttca aattggctgt caacaaacaa aaatttcagg
     6661 aagaagttgc tttctcacaa cgcactatcg acaaaatacc tgttgctgtt gaagtcgctt
     6721 ccaaaaatgt attgtctgaa aatgttgtcg caactggtac cctggaagca tcagacgttt
     6781 taaaccttgt ttcagaaact caaggaaaaa tcattgccat ctacaaagaa aaaggtgaca
     6841 gggtttctgt tggagaggtt attgcaaaag tagatgacga ggtaatatca gccaatgtac
     6901 ttactgcaga agccaattat acacagtttg agaaagatgt tgaacgtctg acccgccttg
     6961 cagaacaaaa tgcagtaacc aaacgcgacc ttgaacaagc taacattggt ttgaaaaaag
     7021 ccaaagccga ccttatcaac gcccgtaaag ccttgaacaa taccagcata aaagcgccta
     7081 tttccggcta tatcaacaat gactttatta ctgttggcca actacttggt ggaggttctc
     7141 cggtttgcga aattgtcaat aacagccagt tgaagctaaa tgtaaaagtt tcggagcgtg
     7201 aagcttacaa aatcaagata ggacaaaacg ttgctgttcg tttatcagtt tttcctgaca
     7261 aaaaattctc aggccgcatt acttctattg ccgaaaaagc tgatgctgca atgaaattca
     7321 acgttgaaat aaccatatca aacaatggcg cacatttgag aagtggtttg tatgccgaag
     7381 ccgaacttcc tgtaaaaaat gaggaaaaca tcattattaa gaaagttagt attattggaa
     7441 gcatggaaaa ccctcaggta tttgtagtag aaggagaaaa agccataaga aaaacgctga
     7501 ttataggcca aagcaacaat aaagaagtag aagtattgag tggtttgact gaaggagaca
     7561 aagttgttgt aagtggccag ctcaatctca aagacggcga taatgttaaa gttatcaact
     7621 aatctgtagg ctttagctgt aacgatgttt tttctttaat aaaatgaaaa acgattaaaa
     7681 atgaaattaa ctgaattatc aataaaacga acaaccatac cagtggtaat ctttgcctta
     7741 ctggctttgg gaggattatt tacatatagt cttttgaaca aggagttgtt cccgaatatc
     7801 gacatgcctg taaacgctgt attgacagta tatccaggcg ctgcaccttc agaagtagaa
     7861 agctctgttt ctgaaaaaat cgaagatgcc atttcaagct tggaaggtat cgacaagatt
     7921 actacttact cttttgaaag catgtctgtg gtggtaatca cttacaaaga tggaattgat
     7981 gcggacttgt cgttgcagga atgtgagcga aaggtcaacg ccataaagtc caatcttcct
     8041 gaaaatatca aagacccgca attcatgaag tttgacttgg ggatgtttcc tgtaatgacc
     8101 attgcggcca atgccaatat tcctgagaaa gaattttatg actttgtgga caatgatatc
     8161 aagacgagga tatcgcaggt aaagggtgtt gcttctgtta gtattattgg tggtaaccaa
     8221 cgcgagattc agataaaagt tgatgctaat aagatagagc aattagggct ttcgcttgtt
     8281 caggtaaaac aaatgattca ggctgccaat gtcgatttcc ccacaggaaa aattaaagat
     8341 gacgatacaa aatatattgt ccgtattgcc gggaagtttg acaaccttga cgaaatcagg
     8401 aacctgattg ttggtgctac taaagatggc tcagtaatca aaataagcga cattgctaca
     8461 gtagttgatg gttcaaaaaa agctaccaag ttggcccgaa acaacggaca gccatctatt
     8521 ggtatgacta ttcaaaaaca aacagacgga aatgcggttg aaatcagtaa agaagtaaac
     8581 aaaatcctgg cagactacga aaaagaatat accaaagaca atcttaaatt caacgtggcc
     8641 agtgatacct cagtatttac tgaccaggcc gttgacggtg tgatggttga ccttgttttt
     8701 gctatcatcc ttgtttctat taccatgcta ttgttcctgc ataccttccg caacctggta
     8761 ttcatctttg tatctatacc aacctcaatt atatcaacct ttacattctt ctggttgttt
     8821 gggttctcgc tcaacttgct gacattgttg gctatgtcaa ttgtagtcgg cgttattgtt
     8881 gatgatgcta tcgttatcct cgaaaacatt taccggcaca tggaaatggg caagagccgc
     8941 tggcaggcta cattggacgc taccaaagaa attggtctta ccgtaacatc tattaccatt
     9001 gtattagttg ccgttttctt gcctataggt ctgacaacag gcattaccgg tcaaatgctg
     9061 cgttcctttg ccctggtgat agttatttca atcctcataa gccttttggt ttcgtttaca
     9121 cttgtccccc ttttaacttc aaggtttgga aaattaaaag aatacaataa gactaagttt
     9181 tttgaccgtt tcttacttgg cttcgagcat gtcattacct gggcaaggtt agtcattctt
     9241 aaagggacac aaatatgcct gaaacataaa gctattacta ttgtaacggc tgtcttctgc
     9301 cttttaggtt catgtagttt gctccaattc gggtttatcc aaacagaaat gatggatgcc
     9361 ggcgaccgtg gcgagtttat tgttactatg gaattggacc gtacggcaac tcttgaacaa
     9421 accagtagta tgtgtctgaa agtagagaaa acactgatgg aacatccaga ggtaactaat
     9481 gttttcacca aagtgggttc aaaaggtgga agtttgtcta ttgccgaaac cccttatgca
     9541 gccgaattta atgtaaaact ggtagataaa aacaaacgag agttaagcac caaactattc
     9601 tcaaaacaac ttcaaaacga gctgaaatcg aagtttgccg ggcctaattt caaaattacc
     9661 gaaatcagta tgatgggaag caccaccact cctattgaaa tttatgtaag gtgcaatgat
     9721 tttgagaaag ccaaagcgtt tagccatatt gtcaccaacg agttaaggag tatcaagggt
     9781 actacagata ttgagtcaac gattgaaaat ggcgacaagg aaatggtggt aaagtttgac
     9841 cgcgagaaac ttgccatatt aggccttacc attggcgaaa ttggcaacca aatgtatatg
     9901 tcgttcgaag gcaaccgcga cctcaaatat cgcgatggca gcaacgaata tgatattctg
     9961 atttcgctgg acgaattcga ccgggaaagt aaagctgacc tcgaaaacat agcttttacc
    10021 aaccatgccg gacaattgat caagctttcg caggtggctg atattattga agctgaaagc
    10081 ccttcgacac ttaaccgata caacaagctc ccatcggtaa ggattaccgg taatatgatt
    10141 ggaaaaacca ttgggaccgt tggtactgaa cttaaagaaa gaatggctaa aattgaaaaa
    10201 cctgcaggcg ttgacatcgt ttatgccgga aacatggaac aacaaagcaa atcgtttgca
    10261 agtatgggta ttgcccttat tgcatcgctt atattcatgt accttatcat ggtagccttg
    10321 tacgatagct atgtttaccc tttggtagtg atgctttcac ttcctctggc cgtcatcggg
    10381 gcattgttgg ccctggcgct gtcgggcaaa agccttagct tgtttgccat catgggtatc
    10441 atcatgctta tgggcttggt agccaaaaat gccatattag tcgttgactt tgccaatggg
    10501 gcgcaggaaa aaggaaagaa agcatttgat gccatcatcg aagctacatc tgttcgtttc
    10561 cgccctattg taatgaccaa ccttgccttg ataataggta tgttacctat cgcattgggc
    10621 tcaggcgctg gtgccgaatg gaaaaacgga cttggctggg tgcttattgg cggtttgaca
    10681 agctccatgc tcctgtcgtt tatcattgta ccagtacttt atgtaacact tgataaatgg
    10741 ataaaaaggt ttaaaaaaaa taatacaccc gaagaatcat cattaataga aaacccctaa
    10801 gtcaatcatt gcttttcaaa aacaatgtcg tttatttagg actccgacag gttctgcaaa
    10861 tgctgtcagg tctgatgtga tactaacccg acagcgtctt tcagacctgt cggcgttttt
    10921 ttagatactt aacaaaacgg cattgacttc aaatgtataa aaacagataa atacctgtct
    10981 tgtagcaatt gtaaaaaaat ccgttcgggg ttcgtccggt taaaaaagag gctgtgcgta
    11041 agacttttca agcttttcac acagcctctt tttatgtata accagaaaaa tatttatttc
    11101 gaaactactt ttaagataga ataatacgct ggcttaggtt gcagctttct gtcaaaagga
    11161 agtggataat tggtccggcc aaaaataggc caattattga gccatgattg gccatcatga
    11221 agcccccaaa atgttacacg acttactacg tccttatgtt ttgcagctac tgtaaaaaaa
    11281 tctgcataac gatttgccag tttttgctga actgaatctg gtaaaccatc tgcataaggg
    11341 ttcatttctt ttttgaaggc aaatgtatca gagatattgg cattattgtt atcccatggg
    11401 ttaggaataa cagtaaaatc gagctctgta aacattactt tcactcctaa ctttgcaaaa
    11461 tcaacaattg cactgtccac aactgacatt gaagggctgt tcacatgcca gtggccttga
    11521 ataccaacac cgcttacttt tatgcctttt gcctgcaagt tcttaatcaa cttaatggct
    11581 ccggctcttt tggcaggttg ttcaatgttg tagtcattgt aatatagctc tgccgaagag
    11641 tctgcttcat aagcatactg aaaagctttt tcaatatact cctcgccggc tatctggtac
    11701 catttcgact ggcggaaagt cccatcttcg ttcaacgctt cgttaactac atcgtaacca
    11761 ttcactttac ctttgtagcg gcttaccact gttgtaatat ggcttttcaa acgtgctaaa
    11821 agagtatctt tactaacatc tttgcctttt gcatcaacaa aaacataagg aggtacctga
    11881 gaatgccaac ataaacagtg cccaacaata aacattttat tcttaaggcc cagctctaca
    11941 aagctatcca ccggggcaaa tacgaatgaa tccatttgcg gatggatacg ctcccacttc
    12001 attacgtttt cgggcacaat agaattaaat tgggagacca acaattgtgt tgctgcagag
    12061 tctttcccgg caatctggtc acggtcaata gctgcaccaa tgtagaaata gtctttgtaa
    12121 gcatctttta agcttgtttg ttttttttca gaacagctta ccaatacaat caatgcaaaa
    12181 gctgcaactg caagattttt catattcatc atatctttag ttttttatgg tataaaaaac
    12241 acccataaat attatgggtg ttttttttaa ttttttaatc atatttttat tctgcactat
    12301 acgacttacg cctttcagcc aattcatcct gcatctggta ttctacgctc ttcttaatct
    12361 tatatgagaa aagaatagtt atcataccaa ctaatgcaat ggccgggaaa atactggtaa
    12421 ttaacctgat acccgaaata gcttcttgcg tttgtacagc attagcaaca tagccatacc
    12481 ctgataacaa atatccggta agggcacctc caagagctaa accaacttta agaccgatta
    12541 caataccagc aaatacaata gctgtagcac gacggttgtt tttccactca gaatagtctg
    12601 caacgtcagc catcatagcc caaggcaaag gcatcgagat accccatgag aagttaaaga
    12661 gcagttgaag tgcaaatgta gcccaaatag ctgttggtga aaggaagata aacatagcag
    12721 caaatacagc agctaaaaca atagtgaaaa taaaagcatc acgtttacca aaccttgcag
    12781 caagcggttt tgagaatata ataccacaaa gagccgccaa ctgtccaaag atattgatta
    12841 tactaaatac attacccgaa atatcagcat ttgaacctaa catacccact ttagctatca
    12901 atccaaataa acacttgttc aatgtttcga ggaatgcaat catgctgtct ttgtcaaggt
    12961 aatatttgaa gaagtacagc aatatgctgt ttcgcatagc aaggaaaata aacatcaaca
    13021 ggaacacaac aaacatggct acccatggct tgttgctcaa taagtctttt atgtcttgtt
    13081 tcaatgatgt ttcttgtttt gggtccggct taatacgttc ttttgtggta aaaaaggtga
    13141 taaagaagaa tactactgcc aataagcaaa aaatacccat agtaacctga tatcctcttg
    13201 agctgtcccc ttgtccgaaa tggttgacca taggaagcgt taacccctga atagctaatg
    13261 tcgcaacagt aacaacaaca aaacgcacag aagacaggct ggtacgttct ttcatatcac
    13321 cagtaattac accgctaagc gctgaataag ggatattgtt cattgaatat attgtcatca
    13381 gaagaatata agtaacatag gcgtaaaaaa gttttcctcc gggacctaaa tcgggcgtgg
    13441 taaatgccaa aaaaccaaaa ataccaaaag gtactgctgt ccatataacc caaggccgat
    13501 acatacccca acgtgtatgt gtccggtctg ctactacccc cataattggg tcaaaaatag
    13561 cgccccacaa acgggcaacc aagaataacc aacctgctgc tgcagctgtt aaaccgaatg
    13621 tatcggtata aaaactaagc tgtaacagca tcatggtctg aaaaataaaa ttagcggctc
    13681 cgtctccaag actaaaacct actttttctt tcattgaaag tttagaatcc attgtttttg
    13741 tttttattat tgtttgtctt atttatatgt ccttttccgg gaaaaatact ccgaaaacat
    13801 tactacgcaa aaaaactcct aatttttcaa tcccaaaaat attatttgaa catcttaaca
    13861 tagattaaat ttctgtcata atgtagcagt aatttgtcga ataatgcaat ttttgggcat
    13921 ttgcactatt aggtaagttg ttcatatatt gtatgttata aaacttatga atgaaatttg
    13981 atagtcacat ttttgcatgt ttttaacact attatttttt actctccgta tttataataa
    14041 acattacagg caccctctga tgataccata caagccccga caggattttc gggcacgcat
    14101 ttattgccat acaagccaca ttgcgaaggc tttttcagcc ctttcaatac ctggccacaa
    14161 atacatcctt ttgcttccac agattgttct gggataacct gaaaaacatg ctcggcatca
    14221 aaacttttat acttttctct taaagacagg gcacttttag ggataatacc aagtccacgc
    14281 caattggcat ctgccggttc aaatatttca tttaccattt gctgggcctt tgggttccct
    14341 tcgggtttta ccacgcgtgt atattgtatt tcaaccgaag gccgattttc atttttttgt
    14401 tttataagca taataacaga ttgcaacaca tctatcggtt caaaaccaga aactacaacc
    14461 cctttaccaa atttttccgg tataaaattg tacatctcag taccggtaat aacacttacg
    14521 tgacccggag ccaaaaaggc atctacagca atgccttctt caatgatagc ctgcatagga
    14581 ggtggcataa ttttgtgcga acttagtaca gaaaaattat ccaacccgag ttcttttgcc
    14641 ttcaaaatac ctgcagctgt tggaggtgta gttgtttcaa atcctatggc aagaaataca
    14701 acctttttgt caggattatt aacagcaaaa tcaatagact caagtaccga ataaaccatc
    14761 ctaacatcag cgccttttgc tttttccttt tcaagtgatg aacttgaccc cgggaccttg
    14821 accaaatcgc cataagtagc aataataaca tcaggaatac gggctaaagc aacagcttta
    14881 tcaatataat caaccggagt aacacatacc gggcagcccg gaccagatac caacacaata
    14941 tgctcaggca acaaatgctt taatccaaaa cgatggatag acatggtgtg actgccacat
    15001 acttccataa tcaccatggg ctctttggct atttgcttta tataagcagc aagttgctga
    15061 accttttcag catcacggta ttcgcttata tatttcattt ctggggaatt tcgtcgttca
    15121 taccaagaat atctatcaac tccaatgttt gtcgggcttc ttcctcatca actttctgaa
    15181 tggcataacc tgcatgaacc aatacatatt ctccaacctg aatattatcg agcaaatcaa
    15241 gtgatgccct gattttattc ccagacaagc taacaacagc ttcattgcca tttatttctt
    15301 caaccttagc aggaatactt aaacacatat acttatttta tttgtttata tctaatacat
    15361 ttatctttct ttattccata tattaatatt cgtatttccc ctcctttaca aggagggggt
    15421 agaggtggtt ttcaaatttt tatacaacaa acctaagtac agttgcccca aagcaaccga
    15481 aacatcattg caaggcacgt ttatattggt taacacttta aaacctcttt cagaaagctt
    15541 attttcagaa ttttctaata aatatttatt ctgaaacaca cctcctgaaa aggctacaat
    15601 atttatctct tttttttgcc ttattttttc tgccatttca acaactacat tgattattgt
    15661 attgtgaaat cgtgcagcaa taatgccttt atctgtgcca ctcaacaatt cttccacaat
    15721 ctggcgtatc acaggaagaa atgaaatctc gtccccatta acacggggag tataaaattt
    15781 ttcatcagaa acaatagctg cttctaatcg cattggagct tcagcatgaa aacttggcaa
    15841 aatacatatt ccagtaaggg cagacacggc atcaaacaac ctgcctgcac tacatgattg
    15901 cgggctattt atctgcttgt caatagatgt aacaacagta ttcaacttaa cagcgtcaat
    15961 agatttaaac atctccaatg gcaggctttt aaaatctttg ccataagcag catacaaata
    16021 cgaaactgct acacgccatg gttccaaagt ggccatatcc cccccgggca aagaaacata
    16081 ctcaaaatga gcaagccttt ccatttctga tttgtgacaa tacaaaaact cgctacccca
    16141 tattttaccg tcaagtccat aacctgttcc atcaaaagca atacccaaaa caggttcagt
    16201 acatttatgt tctgacaaca ccgatgctat atgggcatga tgatgttgaa cttccacaac
    16261 ctttacattc aggctttttg caaaaccggt agtcacataa tcggggtgca tatcgcaaac
    16321 caccatatct gcattgaaac ggaaaagatg cttaaaccgc tcaaaggact caacaaaaaa
    16381 ttggaagttt tcaaaatcct gcaaatctcc caaatgctgg ctcagtatgg ccttatcgcc
    16441 ttttcctaaa gcaaaggccc cagtcaattc tgctccaaca gctacaatac cttctacatt
    16501 atcaacaaca tcaattgact gtggcacgta tccctttgaa cgccttatca aacgaggaat
    16561 gtcattagcc acaaacaaca ccgaatcatc tattcggttg tgtatatccc gattgtatgt
    16621 caaaacccca tccactaaag gcaaaaatgc atttagggct ttttcatcat caataacaat
    16681 aggctcttca gatatatttg cactggtcat taccaatgct tcaaggtttg tatgttgaaa
    16741 aatgagataa tgcaaaggca tataaggcag cattgcccct gttgtcggaa aaccttttga
    16801 aacagaatgc gccaactctt ttttattttt cagaattaca ataggacgcc ggaatgaagt
    16861 caaacattgc tcttcatgat ggttgcaata caaataacct ttaagtgttg atatagaaga
    16921 aaacatcaca gcaaagggct tagtatctct atgctttaca gctcgcaaac gggcaacagc
    16981 tttttcatcc agtgcattac acaccaagtt ataccccccg gtggccttaa aagcataaat
    17041 ctctcccctg cccaatcctt gttgcaattg attaattatg gtttgtaaat tttcagcaga
    17101 atcagcaact acagtttcat catttttctt atataaagaa tatagagggc cacaatggtt
    17161 acaagctata ggctgagcat gaaaccttct gtctgatggg tcttcatact ctttcctgca
    17221 attttcgcac attgtaaaag gtgccattgt agtattgcac cgatcgtaag gtaactcctt
    17281 aacaatcgaa aacctaggcc cacaatgcat acaattgatt aacggataat gaaacctgtc
    17341 agaaggctgc aacatatcat caaggcagtc ttcacaaaca gcaatatcag ggcttacctc
    17401 agtaaaattt tttgaaacat cagtactttg gatgatagaa aagtattcat aatgttctat
    17461 ctcaacatct gaagatttta tcgtttcaat ctcagaatgt acaggttttt tcttttccag
    17521 gtttaactca aacttatgta tagcctcact agtgccttcg acatgaatat tgactgcctc
    17581 atttgtattg tttacccagc ctttcagatt atattcagcg gcaagcctaa atacaaaagg
    17641 cctaaacccc accccttgta tcaacccttt aacggtaaag cgttttgcaa caatagtatt
    17701 attcccttgc acctgaaaaa tatttgcggc aaagttacca ttttaaatca attttgattt
    17761 ttaagattaa atattttaag atatttttgt cttttattaa aggcaaatta tgaatattag
    17821 tgaaattgaa ttattgaacg aaaagcttaa aaacaaaagc ccggaagaag tgattgaaca
    17881 ttttatcaag cttttcggga accgtatagc tttatcttcc agtcttggcg ctgaagatca
    17941 ggttctgact cacatggcct gcaacattga taaaaatgta cggattttca cgctggacac
    18001 aggccgtctt tttcccgaaa cttatgagct cattgataaa acttgtaaac actataacac
    18061 tcaaatagaa atattcttcc ctgaccatca acaagttgaa tgtatggtaa aagaaaaagg
    18121 tattaacctt ttttatgaca gtgttgaaaa cagaaaacta tgctgtaatg tcagaaaaat
    18181 agaaccattg aaaagggctt ttgctggcct gggtgcatgg atatgtggtt tacgcaaaga
    18241 ccagtctgta actcgcttct attctaagct ggttgaatgg gacgaagtaa ataagctggt
    18301 aaaaataaac ccgcttatca actggacaga acaacaagtt tgggattata tccgtataaa
    18361 caacatacct tacaatgctt tgcatgacaa aaacttccca agtattggat gtcagccatg
    18421 tacacgagcc attgtaaccg gagaagatat cagggctgga agatggtggt gggaacaacc
    18481 cgaacaacga gaatgcgggt tgcacaaaaa gtaaacctat ttgtgggctg taataattat
    18541 actggcagta tgataataac cattaccatt aaccacgtta ccagagctta ccggcaaaat
    18601 tgagataatc tgaaaccctt gtttttctaa ttcattgcat ttttcatcaa gttctttggc
    18661 caagtcgtca gagtcaactt ccatctcttc actaaactca tgattagtcc aatatgtaga
    18721 acgcgaatga ataacaattg atttatacat aacaattttt attttacttt taagcaaaat
    18781 aaaaaaaaat attgagaaaa taaaaataat gaaggcctat tttggcaaac agcttattgc
    18841 aatttcaaaa tactttcagc aggtttcccc tctgaacctg aaggtaacgc aatatttaaa
    18901 aacaaagaca atgtaggggc aatatctgca atgcttattt tgtcataaac aaccttgcgg
    18961 cttattctcc atccgtaaaa aattaaaggt acatgcgttt cataagaata gccagtacag
    19021 gatgacgaat tgttggatgt tgtttcggcc cacccgggtt gaagctgata tacaatgttg
    19081 cccgagcgtt tttgataata gctgttttgc atcttagcca aaaccccatc aacataatta
    19141 gagtattgca acattgaagc agtggcaaca ttagccactc cggcaaattg aatcaaaaaa
    19201 ccagccacat cactttcaaa ctttgctata tctatttttg catcttgtat aaggctttcg
    19261 ttcaaatata tctggtgatc cgaaaatttc tttatccaat tgccattacc ataggtagca
    19321 ttcaaaaaac ttttcagcag cgtattggct aaactcaggt taaattcacc agcaggcatt
    19381 cccatatctg tcatcagctt tggcgggtaa gtagccccat gggctgcggt aaaatacacc
    19441 aaaacgttgc ctttaccaac ataactatcc aaaaagtcaa gaagatgttt aatttcttta
    19501 tccaatctga gacaggcatc ttccatctcg cgcgatgcaa tgccaaattg ttttccaatt
    19561 gccccggtag cacaaaagtt gatacagaga taatctggaa tatcatcttt tcccattttt
    19621 tcattcacta tggccgatat tgcaaaatct tttactatat tatcagccat aggcgtttgg
    19681 cgcaaaatat tataatcgcg ttttcctccg gcaattgttg acaatgcttt caaatcataa
    19741 ggaaacgtaa tttgcccacg aatacctctt tcgtaactat tattatctgc aaggctttca
    19801 acataagtac tatcaggtaa cagagttgtc caggtcttat caagatatga atctgcaaac
    19861 ttcttcttgt taaaatcagt tacccaggca ggtaaagttg ttttatatgc agaactggta
    19921 acaaatgtac ccgtttgcac atcaaaccaa tatgcaccat caaccaaatg acccgatagc
    19981 ataaccgaag cagaagggtc tattgaaact gaaaggacta taggctgttt gtaccaggca
    20041 agctttaatt catccccaaa agtactactc atcaattttg atgcagaatg tttttcatta
    20101 ctttcattat ttccaacaca gttgtatttt tcatcacggc tgcaatgctc tgttttatca
    20161 gacaaaccca cataccactc atcagagaca ataccatgaa cagaagggtt agctcctgtt
    20221 gaaatagtgg catatccact attggctcct gaaaacatgt aatcataatg ggcattttta
    20281 catacaaggc cttcgttcat caaacggcgc aagccattag gctcatattt atccataaac
    20341 cggtacagat agtcatatct aagttgatct actacaattc caacaattaa ttttggcctt
    20401 tcagcaggta ttcgttgtgc aaaagaattt tttaaaggaa atacaaaaaa aagtattatc
    20461 agaaataaac aataacgcat ataagattat tttttatcaa ccctgctttt ttcaatagtt
    20521 atactaactg catgcatatg cccccccata gaagggttta acttctgaag cttaagtttt
    20581 acagcatcta tggcattaaa cttttcaaac aagcaatttg caattcggtt cgccacatgc
    20641 tcaatgaggt gcgaggttat agccatttct tgttgaatgg ttttgtaaat gtcaatataa
    20701 tttaccgtat cataaatatt atctgtagaa gccgccttgc taatatctgt catgtacgaa
    20761 agccacacat gatatctgtt accaatcacc ctctcttcgg aaaaacagcc atgaaaagca
    20821 aaaaactcca tcttttcaag ctcaaaccat gctttactca tcgcaatatg gcttatttaa
    20881 aagtattaac tattcatggt aaccaaaaat tcctcattag acgatgtttt agacagtcgg
    20941 tcaattaaga aatccattgc ttctaccggg ttcatatctg acaagtaatt acgcaacacc
    21001 cacatcttgt tgagcatagc cttatcaagc aacaggtctt cgcgacgggt acttgaagca
    21061 ttgagatcaa cagcagggaa gatacgcttg ttagacagct tacggtcgag ctgaagctcc
    21121 atattacctg tacctttgaa ctcttcaaaa ataacatcgt ccatttttga gcctgtttct
    21181 gtaagtgcag tagccaaaat agtaagcgaa ccgccatttt caatgttacg tgcagcacca
    21241 aagaaacgtt taggcttttg cagggcattg gagtcaacac ctcccgagag tatcttacca
    21301 gaagctggag cattggtatt aaaagcacga gctaaacgag tgattgagtc taacaagata
    21361 acaacatcat gcccacattc tacaagtctt ttagcttttt caagaacaat attagcaacc
    21421 ttaacatgcc tttcggctgg ttcgtcaaat gtagatgcta taacttcccc atttacactg
    21481 cgggccatat cggtaacctc ttcaggacgc tcatcgataa gtaaaataat aagatatact
    21541 tccggatggt tctcagcaat tgcatttgca atatctttaa gaagaactgt cttaccagtc
    21601 ttgggttgtg caacaatcaa accacgttgt cccttaccaa ttggcgaaaa catatcaaca
    21661 atacgacacg aaaggttggg ctttgatgct gaaacaagat tgaacttttc atccggaaaa
    21721 agtggtgtca gataatcaaa aggtaccctg tctcggatat aatcagggtc gcgtccattg
    21781 atttcttcta ctttaatcaa aggaaaatat ttctccccct ctcttggagg gcgaattgtt
    21841 cctcttacgg tatctcctgt ttttaaacca aaaagcttaa tttgtgattg tgaaacataa
    21901 atatcatcag gagagttgag gtaattataa tcggctgaac gtaaaaagcc ataaccatca
    21961 ggcataatct caagcactcc aactgcagta actatacctt caaaatcaaa taatttttca
    22021 ggacgtttgt ctgtattttt ctttatttgt atttcctgac gatattcttt agcttgctct
    22081 gtttcagctt ttgtaatttg aggctcatca acgccctcaa cattcgaatt aactgcttca
    22141 atattttcgc tattatcaac aatatcttct acaggaaaat caagaacatc atccagtttc
    22201 acaggctgct ttttagccaa aattaccctg gggcgaatac gcttagattc ttcgtcctgg
    22261 ttaacaggtg gaactgtaac tgtttctgtc tgaagcggca ttgggttaac ttttacttct
    22321 atcttgttaa cgggttcatt aataattttt tcttcagcaa cagcaggccc ttgttcagat
    22381 tcagatttct ttttacggcc tcgtttattt tttacaggtt cacttccgtc agaagtccct
    22441 tggacagcag aaacttcttt tttcttcttg ctattggtaa cttcaatagc ttgttggtca
    22501 aggatgcgat aaatcaaatc ttgcttactt aaattgtctg tttttttaat actaagcttt
    22561 tgagctattt ctttcaattc aggtaaagat ttttcattta attcgataat atcgtacata
    22621 aaaaatggat tatgttattt gattgtttaa tacaaaacaa aaggaattta ctcaggaggt
    22681 aaggttttgt tggtgcaata ttaagcaatt gattgtaaac ttcaaaaaaa attgcactca
    22741 atattaataa aaataaaata actactttta ttcaacctgt ataacccatt aaataccaaa
    22801 aagaaaactc aaacattggc aaaaatgtaa aaagaaaaaa caattaaaat ttaaaaattt
    22861 attacccaaa ttcttaaaaa tatgcactaa tttagtcatc ttattttcgg gcttacttgt
    22921 tggtcgaagt gctgttgtcc ctaagcaaag aaaatattcc ccctaaaaat aaacacatgc
    22981 gacagttaaa aattgtaaaa caagttacta accgtgaaac gccttcgctc gacaaatatc
    23041 tacacgatat tggcaaagtt gagttgatta ctgctgaaga agaagttgaa ttagcaaaaa
    23101 aaattaaaaa tggagacgaa aaagcccttg ataagctggt aaatgccaat ctgcggtttg
    23161 tggtttctgt tgccaaacaa tatcaaaacc aagggcttag cctgcctgac cttattaacg
    23221 aaggaaacgt agggcttata aaagctgccc aacgttttga cgagacccgc ggtttcaagt
    23281 ttatctctta tgctgtatgg tggatcaggc aatccattct tcaagctctt gctgagcaag
    23341 cccgtatcgt caggctccct ttaaataaaa ttggttcaat caacaaaatc aacaaaacat
    23401 tttcaaaact cgagcaggaa tttgaacgag aacctaccat cctcgaactt tcccaggccc
    23461 ttgaacttgc ccctgccgat attaaagatg ccatgcgtac ttcgggtcgt catatatcta
    23521 tggatgcacc tattgggcaa gatgatgatg gcgacatgta tgatatttac ctaaacaatg
    23581 acgttcccag ccctgaccgc agccttatta cggattcgtt gcgccacgaa attgaaaggg
    23641 ccctttctac cctgccagac agagattctg acattatccg ctattatttt gggctaagcg
    23701 gacaaagccc cttaacactt gaagaaatag gcgaaaaact taaccttacc cgcgaaagag
    23761 tccgtcaaat aaaggaaaaa gctataaaaa ggctcaaaac aacaactcga ggctctctgc
    23821 ttaaaacata tcttggttaa aatttattaa acgttcggaa ggaccaataa aaaattgaca
    23881 tgtaaaggca cttgttattc aagtttttta cctttgcaga aaatagcaga gaatgagtta
    23941 tattttatca ccttctatat taaattcaga ttttttgagg ttatccgata gtattgaaat
    24001 gcttaacaac agtgaagccg actggataca tattgatgtt atggacggta gtttcgtacc
    24061 caatattagt attgggttac cagttgttga agctatcaat agcactgcca aaaagccttt
    24121 ggacgtacac ttaatgattg ttaacccgga taattatgta gaaaggtttt acgaagccgg
    24181 ggctaaaatc ctgacagtcc attatgaagc ctgtcctcat ttgcaccgta cgttgcaaca
    24241 aatcaaagct aaagggatga aggctggcgt tgctatcaat cctcacaccc ctgtgtctgt
    24301 gcttgaagaa atcattccat ttactgacat ggttcttaat atgaccgtca atccaggatt
    24361 tggaggacag aagttcattg aaacttctct taaaaaaata gaaaagctta aaagcctgat
    24421 aacaaaaatg aacgcacaaa cacttattca ggtagatggt ggcgttaata gctcaaattt
    24481 aaagcaattg cttaatgttg gcgtagatgt aattgttgct ggtagcgtga tatttcaagc
    24541 tcccaatcct tcagctacca tcaaagaact taaacaaata tcacgataac ttttaaaggt
    24601 ttgcatctat atgaggaaag tcctgttttt gctgcttatt ctgactgcca tttgtggttg
    24661 caaaagcaaa ccaacacctc cgaaagtaga aaaaataccc cttactgttc tgcgttttga
    24721 caaagacctt tttgctattg attatgcaac acttgaagat tccctgccct ttttcagaaa
    24781 aaaatatggc gagttttttg acatctttaa ttttaaaatt attcagattg gcagtagcga
    24841 aaataaaggc tatgcagacc ttttaaaagg gtttgtaaca gactacacta ttaacaaggc
    24901 aaaaaaagaa tgtgacagtg tatttgccga ttttaacact attgccaaca atataacttc
    24961 aagtatgtca tattttcatt attattttcc tcaaaaacct gtacctacta ttattactta
    25021 tgtatctggc ttcaaccaat ctatggtaac aactgacact atacttggaa tagctttaga
    25081 cagatatttg ggcgaaaatc atgattttta caagcgtatg gcattaccaa aatacataag
    25141 gctaaagatg aacaaaagca ggattccggc tgatgtggta agagcctggc ttatcacaaa
    25201 ctttccaatg aatgactcag catttaacct gctttcaaac atcatctatc ttggaaagat
    25261 agcttatgca agctcattac ttcaacctaa tgccgaagat actgtattta cagggctaag
    25321 caaagatgaa attaaatggt gcaaagaaaa tgagaagaac atgtggacat ttcttgttga
    25381 aaagaaaaag cttttcagct cagatatgat gcttatcaga aaatattgcg aagaagctcc
    25441 ttttaccaag gactttgggc aagagtcgcc cggaaaagca gtaatatgga taggttataa
    25501 tattgttaac gcgtatatgg ctaataatcc tcaggttgac ttacaacgac tgatggaaat
    25561 gaatgattac atggctatcc ttaaaagttc aaaatacaaa ccataaaaaa aggctacccg
    25621 aaagtagcct tgatttttta cagctaaact attaagcctg gctaatagcg ctaaccggac
    25681 aaacatcagc acaagcgcca cagtctgtac atacttcagg gtcaatcttg tagatttgtc
    25741 cttctgagat agcttctacc gggcattcgc tgatacaagt gccacaagct gtgcaatcat
    25801 ttgaaatttt ataagccatt gttttaaaat ttaaagtttg tttcaacaat taagtatgca
    25861 aatgtagaga aaaagcaact ctcctacaaa acatttttca atcaaaaact tctatagcct
    25921 gaataaaatc atgcacatgg ctataacaat gaacattatg agttttttga aaactaaaat
    25981 tttttatttg caaaccggtc aaaataactt tagtatgagg gaaactcaat aaaatattac
    26041 gcacaatttt ttcagcatta accctaacac tagaaagggt gaacgatgtt actaacacat
    26101 ctaccgaaag cttctcggat aaaccgggaa aattgtccat aggtacattt tgtccgagat
    26161 aaacagtaga atagcctcgt tttctcaata aatactggat aaaaagcaaa ccaagctcat
    26221 gcatctcacc ttcaggaagg aaaagtaaaa aattactttt gcgagttatt ggcacatcgc
    26281 gctgactgtc tattgcaatt ataagcttct gcctgatgag cgaagataca aaatgttcct
    26341 gggctggcaa gatagtccct gtctgccata aaatgcctac tttttcaaaa aacggtgtca
    26401 atactttaat gtaagtatct tcaaaaccaa attttataat taagtttgac aatatcctgt
    26461 caaacttatt ctcatcaaat tcaatcatgg ccgaaataag cccttctgat aaatcttcta
    26521 tcttagaagt tgtttgagca agaatagcca ctttctcgcc cagcacactg tccggcaaac
    26581 gcgaaatctg cgatatacgc atcccatttc tgactaaaat agaaatattg agaagttttc
    26641 gcaattcggc atcagaatac aagcgaatgt tagtatttgt tctcgacgga caaattaaag
    26701 aatagcgctt ttcccatata cgtatggtat gggccttaat gccactaagt ttttccatat
    26761 ctttaattga gtaggtcgcc attttctggt tttacaatta aacacataaa gataaaaaag
    26821 gttcagaaga agaaatcaga agtttaagaa ggttaaggaa catatttgct aatttgctaa
    26881 tttgctaatt tgctaatttt atttctcaaa tgagtctgta actttcaaca aacgcatgaa
    26941 acgcctgcga ttgagacgat tgagacgata tgtttcgaag taagcaatga tacgcttgag
    27001 tacgtcaaaa tacaacacaa ccattaataa ccctgaaaaa aaccataaaa tgaaaacatt
    27061 aaaccaaaat gtatctatca accatttgcc aactcttttt acaggagcat aataatgggc
    27121 ccttccattg gaagaataag gatacctgta aactgcattt tttataggaa gaatcttacc
    27181 attctgaata ctaaaatcgt aaagttcttt ttcattggta acaacaaaag ccagttgttt
    27241 attgaaatat ttctgcttta gctttaaaac ttcttctact gagcctgtct tttttactaa
    27301 agcctttaca acactatcac gtttagaagt agaatattga taccaaacta aaaacctttt
    27361 attggcattt tcaagaaaca acctaatttc tgcaatctgt ttatcatcag gataagttga
    27421 aggaacaatg ttttcaggca aagaagagcc taacaacaaa gacataactt taacttcatt
    27481 gcatacaaca gtcatattat tcaaaaccaa agaagaatcg gccttatttg ttttatgggc
    27541 ttttgcttca tcaaccaatt catacaactt aggtataaca tacgactgat aatagttggc
    27601 atacttttga agctgttcat actgaaaaaa atgcttttca taaggattgt ctttgaactg
    27661 cgttacagcg agcgcctcgt atgcccaacg agataccata aactcaccta tcacaggcac
    27721 ctccatatct gaggctattt tgttgtgcat tttatcaaaa tcaaccacaa cgccactaaa
    27781 caaaagctgc ggaacaagta tcagcggaat taaaatgtat atggtaacta cagaattaag
    27841 gcctgccgaa atgttaagcc caaccatatt ggcccagcat gaagctgaaa acaaaatcac
    27901 ccagtacctc cagttcatcc cctcgatatg cagcacgtga ttgccaatcc aaacaaacaa
    27961 aaacatttga atagccgaaa tggtaaacat aatagccact ttggaaaaca aataacttga
    28021 acggcttaaa ttaagaaact tctcacgttt taaaatatta cggtctttaa aaatctcttc
    28081 tgcactgatt atcgagccca aaaagataga gacaataacg gccataaaca aaaatgcagg
    28141 gatattcgtg ttttcgccaa aaatatattg tgccatccca tcaacaaaaa caaacctttt
    28201 tgtaaagaaa gctagtatca gagccaacaa aggcgcttca attgctgtca aaagcaggta
    28261 ttgttcattc tttattttgg ccagcacatc acgttttaaa aaaatgacaa actgtttaaa
    28321 atgccctgga attttaaaag catttttagg aatgctgcca tcatgttcgc gatatatttg
    28381 ccttatttca gggtcaatat ttttaagata aagctggtac cactcttccg gggtagtctt
    28441 tcttttacgg gtcaaacggc cattagcatc aactacacga gcttcaacgg tacgcaatat
    28501 ctggtctggg ttgatatttc cacaactcag gcactcactt tcttcggcat ctgcaaagtg
    28561 tgcaattctt ttaaaatagg taataccatc aacagggttc ccataatata tgatacgtcc
    28621 tccctgatcc acaaccagta atttgtcgaa catcttgaac acgtcagatg acggctggtg
    28681 gatattggca acaatcatat tgcctttaaa agtctgcttt ttcaaaaggt ttattacctt
    28741 ttcggagtca gcagaagaca atcccgacgt aggttcgtca acaaaaagga tagaaggctc
    28801 gcggataagt tcaagagcca aattcaggcg tttacgctgc cctccgctca atattgttgt
    28861 aaaagcatca cctacattca aatctctggc ttcgacaagg tcaaagtcaa ctaaagcatg
    28921 ttctacaaca cggtttattt cttcttcgct ataatcgttg aaacaaagct tagcattgta
    28981 atacaagttt tgatatacgg taagttcttt tatcaacaag tcatcctgtg gcacaaaccc
    29041 aattactcct ttgagctctt ctttgtattg gtgtatatcg tagccgttaa ccttgataga
    29101 accactgcgg ggcttaatgt tcccgcacaa tacatttagc aacgtagatt tacccgaacc
    29161 actgcctcca ataatgccaa taaacctgcc tgactcctcg ttaagggtaa aacgacgaag
    29221 gccgtttgta ctgtttccat aacggaactc tatatcttca gcagtatata caaatttact
    29281 ttcgatattg gcctggataa aacggccggc catgcgggta taatacaacg agccaacctt
    29341 ggggttcttg ataactgccc caactaccca aatatatgaa cgctggggtt tgacataatg
    29401 accgttgaga taaaaggtaa ggttgccgtc ataacgaaaa acataagtgt tggtaatagg
    29461 taaatgtata acagcaatgc ggccttccat tttactatta tacatttgct ttatatcggc
    29521 atattcagga gtcgggtttg agtctataaa aagaatgttg tctttgtgga taatttttgc
    29581 aaactctcca aaagtaaagg ctcgcaaatc tttaaactca tcaatattaa ttttgagata
    29641 aatggcaact ttaaaaacaa acttatgttc ttttagtgta attgcttctc ccctattgat
    29701 aaaatcgagc aaatagacca aaacaatcat tttttgttcc atttcaagct caacgttaat
    29761 cttttcgcat atctcctgaa ttttttcttc gttcatgttg tgctgatgcg ccgtagcatc
    29821 atcatttgca tacatggcct caggatggaa aaggcgtacc tggtcgtcaa aatactggat
    29881 gtacttgtcc accagctctt ggctatattg cctgtcaaga taatcaataa caatatcgcg
    29941 tttgttgcca aaattaccat cagccttaac atcggccacg atagcaaaaa ggcgcatcag
    30001 cgattttaat attgactcgt tcattgtttg gttaattatt caacaaaaca gccctaacct
    30061 tttcaggaca gggctgcttg aaacttttta tccaacaaat tacattacaa atgtttttct
    30121 aacagcctct acggtattta ggatcaaact gtaatcttgt tcggttagac cagattttac
    30181 tttgtcaaaa gctggcttca acaccattag tttttgttgt atatcgttaa tgtctttatt
    30241 gttattatat ctgcccaaaa gctccataag tttattgtat gattcgcttt gttttgaaac
    30301 aatatcaaca atctgagtat tattgtatgt atcttttgaa atgtgtgttg caatgtacat
    30361 caactctatc cacatgccag aaaccagcat agctgaaagc tcaggtttat tctgtttgtt
    30421 aagaaatgaa tatgtttcat aaatagtgtt ggtaaccact ttaacaaggc tgtctttgtt
    30481 gttaagcttt gctttgaggt tttcgtcaat aagcgggctg taatctactt tgatacctaa
    30541 gtcatcaatc aaagctttta ccggttcaag gtaaatcttg atatcctgct ctttgttgta
    30601 tgttgaagca taagccaaat cagcgccata aacacccagg ttaatagcct tatccttttc
    30661 agttaaatat ttttcaactt tattaggggc attaaggttt tttaccaaat aagcagcccc
    30721 aatttcatta agcattttgg tcatttcaaa tgggcttggc aagggataaa caatagcctc
    30781 aacattttct gtaatttcat ttttgttcaa tgaatctgat gttgctgttc cctgtggatt
    30841 atttgaactc tggcatgaag ctaatgaaat gaaaatagcc acccctacaa ggcctaaagc
    30901 atttaatatg tttctctttg ttgtcataat gttagtaatt ttattattct ttcggccaac
    30961 aaaattaact gttttttcgg catttcaaat tttttatttt aaaagaaaag ttaattccat
    31021 ataaaatttt aaagtacaaa aatatccact tactaatcaa gtggcgcatg cataaaacac
    31081 cgcaaactaa agaaatatta agaatttaac aagtatttac catgaacatt tacccactta
    31141 tttatatatt tacctcctga atttttttca accctacata agggtaatca taaattcagt
    31201 tgtggatact ataaaggcgt aatatgactc ataataatat aaatattaca gacatcttac
    31261 tttcaaaata cttttcaggc aatgccagcg aagaagaagc caatgctatt cttatgtgga
    31321 aaaaggcctc tgaagcaaac agaaaggaat ttgaccagtc atatcaggta tggcagctga
    31381 ccatgactgc cggcatctcg aaacttgacc ctgatgcagc ctggaacaag gtcaatgcca
    31441 gggctaaagt gacaaaaaag ccaacattca aagttcaaag gttttatagc aatatgctca
    31501 aagttgcggc ggtgcttgtt gtatccctat taacttggtt tacctacgac agatacttct
    31561 caactgtccg atattacgct gatgcaaata ttgaaaatgt aattttagaa gatgggacaa
    31621 atgttacatt aaataaaaat tcgtggattg aagcaccaaa gcactttcgg ggttcattaa
    31681 gaaaggtaag catgacaggt gaagcatttt ttaatgttaa gcatgacgct tcccacccgt
    31741 ttattatcga aacaaaaaat gctgccataa aagtactggg gacctctttc aatgtaaaaa
    31801 caatgccgga aggcaacatt aatgttactg tagctaccgg cagggttgct tttatgcctc
    31861 acaacacaaa agaggctgta atcctttcaa aaggtgaata tgtaagctgg aatgaatcaa
    31921 agaaagagct tagaaaagcc cgtagcgcta accaaaacta tttagcttgg aaaactcaca
    31981 ggtttgagtt taaagaaaat aagctggaca gtgtaatcaa agccattaat gcaatttata
    32041 attctgaaat tgcccttggc aaagaggttt cacaacttaa gcttactgcc aggttcgaca
    32101 gcctttctat tgagcaaatt gttgaaacat tgaaagttac ttttaattta aaagctgaaa
    32161 acaccaatac tggccttgtc cttgtaaaaa aataattggc cctaaccaga acaaccctta
    32221 aaacatgagc tttaggtggg taattataag catattattt ttacaatgca gcattcagct
    32281 tacagctcaa gccaaccgga ctattaatct atgttttgaa aatattgaaa ttgctaaagc
    32341 cattgacacc atatccggca aatatcatgt caacttctct tacaatgcag aactcgacgt
    32401 attgaaaaaa aagaaaaaag tttgtggcga atatacttta tcttcagctc ttcaggcact
    32461 cctcaacggt gaaaatacag gctattcaat tatttccaac acagtagtat tgtttcagtt
    32521 aatcaccccg gatgccgact ctatacaaaa tataagccct ccaaagcctg agtttattga
    32581 tattacaggc aaaatatcaa atgcaacaga caaaagccca atttcttatg cctctgtatc
    32641 ggtaaacaaa tatccaatct caacagtttg caacaacgac gggtatttta aacttcgtct
    32701 gcccgaaagg tattataatg ataccattgt aatatcaagt ataggttata tgcaagaaaa
    32761 aatagctgtt tctgatttta atggacaaat acgcaacatc aaactttttg aatctgttgt
    32821 gaatatcaac ccggtgataa ttaaacaaat aagcgccgag aatatagtaa aagagtcttt
    32881 aagccggata aaaaacaact tttgccaaag aaaagcggtt tatacatcgt attaccgcga
    32941 aactataaaa gaaaatgaag agtattactc tgtagccgaa ggtgtactta atattgccaa
    33001 agccccttac aacagtcata ctcaaattga ccaggtaaaa atattcaaag cccgtaaaaa
    33061 tgaaaacaag ctaaaactaa cgtctgtcat ttataagttg gaaggaggcg ttgccaattg
    33121 tgtaaaaatg gatatagtca aagacgaagc tcctttttat tcgagtgaag ccttcaatac
    33181 atacaaatac caggttgaca gaatggacaa ttacaacgga aggcttttgt acattatttc
    33241 atttaaacca tactccgata cagaagactt gacttacaca ggaaaactgt atatcgacaa
    33301 agaaagcttt gctcttgttg ctgcaacatt taaacttagc ccttcgggct tagaaagggc
    33361 aactcagctt ttggtcaaaa agactccccg taaaaccagg ataaaacctt acaacactga
    33421 ctatcgtgtt ttttacagag aaataaacgg caaatggttt ctcgatttca cgtacactgg
    33481 catagaactg aaagcaagga gcaaaattat tggcttcaac tcaacatttt cggccatttc
    33541 gcaaatggtg gtaaacaata tcgacactac taatactgaa cgttacaaat accgtgacat
    33601 atcaaaggtg caagacatct ttaccgaaca aattggcgaa cctgacaaaa acttttgggg
    33661 cgaatgcaat attattgaac ctgaagaatc tttgcttgaa gccatcaaac gcatcagggt
    33721 agaatcagtt caaaccaaaa gccaggggtt ctggcaaaag atttttgggt tgtaagttga
    33781 acaaatcaac taattatttt gcaaccaatg caaagcctct ttagtatcca aaactttctt
    33841 tacaggaatt tttatatgat gttcatcaaa atagccaacc aaagcatcta cagtttgtga
    33901 ttgtttgcga ttagcaataa caacggctac ttttgaattc acaaactttt cagaatgagt
    33961 tttgaataca ttggcaaaag catctacctg cttagtttca aaaagccgtt ttcctttagt
    34021 tacatcgcaa atccaatgat aaccctcaac agcttgttct ttctcaatca atgctttgaa
    34081 tgttttatgg atatcattgg tacaaatttc gccccaaaac cggataaata ccattttttt
    34141 ttcttcatct atctgataat gagatttttt tctttcaaat aatccaaaca taggtcaggt
    34201 caaataattt tatcaaacat aagcaaaagt atggtctttt aatcacaaaa atgagcaaca
    34261 tcatcaaaat attcaggaag agtttgggta aaaagcctaa aaaagctaac cgaaaagggc
    34321 tgtaattttt gcaaacaaac acaaagtgtt gccaatcaat attttactca acttatttaa
    34381 aatacaagct tattaagcaa gatatacaaa ataaaccggg tccctgcaat gcgtggttta
    34441 tttagaatgt tgtccaaaag cccaatgaca acaaaaaaaa caaccattgc cctggcaaaa
    34501 aacaaggcca atggttgtta ttattttgaa actccgaaaa gcttattcaa gaggtttgat
    34561 gaattgcatc agctctctgt catggttgtt tacaatttta cattcgttat caaaatacat
    34621 ggtagctccg ttttgggcag tataaggttc ccattgtggc aacatccctt ctacgttagg
    34681 attggctgtt tttataaaat ttatccacgc agaactcatt ttgtcagcta acttataggc
    34741 ttcaggggta gtacttgtcc agtcagtacg catatcaaca ttattgaagg ccaaaggaat
    34801 atccaaacca tgaaacgcac cctttgaagc gctgtcaacg gggctcttcc atgccagcaa
    34861 atacatatac aatggggcac ttgtttcttt tgcccttgca tcggctgttc taatagtata
    34921 aggcctgaaa actttatcaa tagagagaag gtcctgagga gtataatcgg gatatgcctt
    34981 tgcaaatagc tttatgtatt gttctgtttt atttccatat tcttttgcca gacgttgttt
    35041 tgcctgctcc agggttaatt ttttgtcggc ataagcagtt ggcatcagtt cgttaaaagt
    35101 agaacccatc ataaggggaa tatcttttga gaaatctgca aaaccagggc tgaaaggttg
    35161 ctgaattaaa acaacaccat ctgcagaggg tgcaaaacca aacattgtag gcgacccggg
    35221 ttttctcggg ccggcaattt ttgcaatagc atcattcccg gcttttacaa gagccaaata
    35281 tgagatagta tcgagctttt gcacatcttc ttttttcaag cccaaatttt caagtactgc
    35341 taaacccaag ctttgcgatt tttctttatt cattgtgttg ataagtgttc cactttgaat
    35401 aatagccttg tgaaacaaac cttttgcagc cggcatacac atgagagtgc caacttttcc
    35461 gcctccgcct gattcgccaa caatggtaac atcagaaggg ttgcctccaa aattttcaat
    35521 gttattttta acccattgca gggcctttac aatgtcgagc ataccaacgt tggctgattg
    35581 agcatatttt tcgccacaag ccgacaaatc cagaaaccca agaatattca gccggtgatt
    35641 aaccgatact acaacaatgt cacctttttt ggctaaagcc tgaccataag tcatcggatc
    35701 attactgctt cccacatgaa aaccgccgcc atgcaaccaa accattaccg gacgtttttt
    35761 gccgtcatta atgccttgtg tccatacatt gaggttaaat agtttttttt cgtcctgggt
    35821 agaatcagga atccaaggca caatttgttt ggcaacaaag ccaaaatttt tacattctaa
    35881 aacattgtcc caggcatcgg ggtcttgtgg gggcataaac ctttcggctt tggcataggg
    35941 tatgccttta aaagcatata cagtgtcgct ataactccca cgcacctcac caaaggtggt
    36001 tttaacaatg ggttcgaggg tgctgccggc ctttgtcttg cagcctgaaa tgcaacacac
    36061 tcctgcaacc atcaagaaga gcataaaatt tttaaaatgt ctcatagtat tgtttgttta
    36121 tgttgtgaat atgtaataaa tgttaaaaat acattttatt ttctttgtct tgaaaaaata
    36181 aaaagattta aatctatttt aaccctattg atgtaattag ggttaaaaag aattgaattt
    36241 caaaaaatta acaacatttc aaacaatttc taaatcagtt tgtttcgtcc atgacttgag
    36301 ggttatgtaa cttgtcataa aacccaatga gatattttga agataataaa tatgaatcat
    36361 acagggactt gtttttacca atataacaag caagggccgt tgaaagagta caaccggtac
    36421 catgactgta cgagaatttt ttacgctttc tttgaaaatg gtacacatct gttggggtaa
    36481 tcaacgcttc tttaagcaat ttatcgctaa aatgtcctcc ctttaacaaa acagaggtat
    36541 tccattcctt acatttttca attgctattt caatagcttc ttcaatttta ttgatttttt
    36601 gattggtaag aatctcaaat tcaagtttat taggagttat taaatcggtt aaaggaaaca
    36661 atgcttgttg taaaaccttc aatgacgatt tgtctaaaaa tggcacccct cccgtagaaa
    36721 ataaaactgg gtcgaggatt acatgttttt gagaaaattg ttttaaacaa tcaacaatag
    36781 ccaataaatt gtcgtgactg caaatagccc caattttaac agttttaacc ggaaatgaga
    36841 gaaagcattg ctctatttga aatttcaaca agcttgtttg cacggcctca accttaaaaa
    36901 ctttattaaa gttttgcacg gtaatgccgg taatagccga aagtggccaa taacccaaat
    36961 tataagctac tttaatatct tgttggatac cggcaccacc gctgttgtca gaggctgcaa
    37021 tagtcaaaaa ataatccatc tatatatttt tatcattttt aacggcacac aaactgccac
    37081 acattgtaca gtagtcttca ttactgcttt ccgattccaa ttttcgtttt tgtgccattt
    37141 caggatcaag ggcattgata aatatagtgt cccagtctaa ctcacggcga gcctttgaaa
    37201 caacataatc acgttgacgg ctgccataga tgttttttac aatatcggcg ctatgggctg
    37261 caattttgcc agccataaca ccttctttta catcggatat attgggtaag cacaaatgct
    37321 cggccggggt tacataacac aaaaaatcgg ctccataaat agcagcaata gtaccgccaa
    37381 tggcccctac tatatggtca taacccgggg ccgaatcggt taccaatggg cccaaaacat
    37441 agaagggggc attgtgacaa agttttttca tcagtctcat atttatttct atttgatcta
    37501 aaggcatgtg ccccggccct tcaaccataa cctgcactcc catttctcgt gcacggtcaa
    37561 ccaattcgcc aagaattaat aattcagata tttgtcccct gtcggtagca tcggctccac
    37621 aacctggccg taacccatct cctaaactga tggtaacatc atattttttg catatttcaa
    37681 gtatcctgtc gtattgctca taaagagggt tttctttctt gttatccaat atccatcgtt
    37741 tcaacaaagc cccgccacgg cttactatac cgcaaacccg tttgtcattg tctaaaaacg
    37801 aaagagaatg cctggtaacc ccacaatgaa gtgtcataaa atcaacgccc atctgggcct
    37861 gaatttcaat ttcattaaac aaatcttcgg gttcaagctg tgctattctt ttgccttcag
    37921 acagtaaacg ggctactact ccataaacag gtacagtacc aaccattact gaagattctg
    37981 atataatttg ttgtaagatt ttgtgaaggt tgccccctgt agataaatcc atgatagcat
    38041 cggcgccata ctttattgca accttcattt tttccatttc ttcagaaata ttgcaatgtt
    38101 tttcggatgt accaatattt gcattaatct ttgttgataa acctttgcca atacctcgaa
    38161 ccgggaacgt atggtttttg tttttgggaa ttactatccg accaaaagct atttcatctc
    38221 ttaaccaatt aacatctaca ttttctttat ttgccacttc aagcatttcg ggggtaataa
    38281 tgccttcttt tgctttttgt atttgagtac tcatcttttt ctttttaata gattgtttaa
    38341 atcttggatt cttttgtcaa attcgtcggt ttgcataaaa tgacgtacca ttgcaatgtt
    38401 ttgtgcacca gcatcaatta cctgttcaat gttttcgggg aaaatacctc ctatggcaac
    38461 tactggtaca tgtgaaaaac ccaaaacctg tttcaattgt tctgtaccaa ccggtttatc
    38521 gggtttggct tttgcattag taggataaat tgggccaaaa ccaatataat cgggtttttt
    38581 ctttaatgct tcttttgctt gttctattga atgggtagat agacctattt tcatgttgcc
    38641 aactattttt cgggcatctt caacagaaat atcgtcttgt ccaaggtgta aataatctgc
    38701 atcgcaaata gctgcaatat ctggccgatc atttacaaca aaattagtag atgtaccttt
    38761 agttatactg cgtatgtttt ttgctatttt tatcaattct ttatcagaaa ggtgtttttc
    38821 gcgcagttgc aacatctgta tgccatgggc cacacatttt tcagcaattg cagaatatga
    38881 caagtatggt tttgtgataa ttatatatat gccaaagttc tccatattat tttaattttt
    38941 cagcaatcaa atcggcaact tttttacctg aaagaagcat cccgccaaaa attgggccca
    39001 tcctgaaccc tccactgact ccgtttgatg ccataccaga cacataaagc cccgggaaaa
    39061 tttcttttgt attctcaaca gtagtacgct cggcttcatc aacagaaaga gaacgttctc
    39121 ccattacttt cccagtggcg gtattcaatt ttatgttatt tttacgttca agggtacggg
    39181 caatgtcaca atcgtggccg gttccgtcta ttaccgcttt tgccattata aacaatgggt
    39241 caacgtgcat gtgttctcga tgtacaggcg accagtttaa aacaatcccg caaactttat
    39301 tatcttgaaa tacaacatct tcaattgatg tacaattgaa aatggttact cctgatttgg
    39361 ttgcatgata tataagggca gatgtagcat gtaccgaatc taacgtataa taatcttttt
    39421 caaagtgctt atatgtaata tgaaattcat caagaataaa taaagcttct ttttgcacca
    39481 ctatttcatt gaacatcata gcgccgcccc acatgccccc tcccggagca agctttcgtt
    39541 catataaagc aacctttaag cctttttgtg ctaaataata agatgcaact aacccggatg
    39601 ggcctcctcc aacaatagca acatcaaccg ataaattttc tttgagtttt tcaaaatacg
    39661 aatcaacaat tccttttgat acaatttgtt ccattttact gtttatttaa gttaaaaaaa
    39721 attaaataaa caaggaataa ccttaattaa tggttgcttc gtatatttca ctttcctacg
    39781 caggtattat cctgatcagg ttaaagggta taatctcagc cttaataagg cacccctagt
    39841 ttattttgca caaatgtatg acaatttttt taatcttctt tataaacagg agggttaacc
    39901 ccaattattg aaataccatt tttaatgaag actttttgag ccgtaaaata tcaaagagtt
    39961 tattttttat ttctccaaaa ctgcacaaag gctttttgtt tcataatgcc tactggccct
    40021 tcgtagatgc ctccacctcc gcctgagtca taaactcgta cagctaatgt attttcttta
    40081 ttagggataa gtaagctacc atctatataa tatactctta attggttgta acgtgtcccg
    40141 gcatccatac tgataagggt ctttggggtg ccaatgtagg tgccgttgag gtacacctcg
    40201 tcctggtcat ctatttttcc aagcactata accactctgt cgttggccag accttctgag
    40261 gctacaaatg tttttcggta ccaggcaaaa ccgtcatagt ctttgtatcc ttgctcttcc
    40321 caatatttag ggacgtataa cttgttccaa cgcgaatcgt cgaatgttgg ttttttataa
    40381 tcagctttgt ccccgacttt aaattgccac ataccgctaa ggttaatttc gaaaggaggt
    40441 ttttgttctc ctaaaaacaa agctatatcg ccatcaacaa tacctccttc gcgttcgcca
    40501 tcataaactt ttactacaat ggtattatat cttcctatat tgacatactc tgatggaatt
    40561 acatatttgc ggtaaacatt atatgcagtt gaaaagtccg gaggaaaagt acccgaaaag
    40621 ccaaccagct tgccgttaaa atatacctcg tcacaatcgt ctattcggcc caaagaaagt
    40681 attaagtccc tgtcttcggg aaaatcttta attactacct ttttccgata gcaggcatat
    40741 ccgtcatatc cgtaaaaccc ttggtcttcc cagtttttcg ggacaaacac ttcgtcccag
    40801 tcggcatcgt tgtactctgg ttttatccat tcgtttttga agccaagtgc aaacttccaa
    40861 ctccctctca ggctggcaat tcttgtatca ccagctttag atgtaaagct gaggacaagt
    40921 aagcaaccca atattaacca tcttctgcac attgtcggca attttcaaaa tatcattcaa
    40981 aaaaatttcc aaaataaaag ttttctactc ctttgatttg gttttcgttt tttcggcgca
    41041 tatcttccca gctgttttta ttggtaatac ctattggccc ttcgtaaata ccgcctactc
    41101 cgccatggtc aaaaaccctg actgcaatta catttattcc gggtttcaaa aggtttttag
    41161 gtattttata acctcgcaga atcttgtaaa gatcgtgatt atgaaagtag gtattgccaa
    41221 ataatgtctt gctccagttt tggccaatga gtttcccgtt gacgtaaacc tggtcgtagt
    41281 cgtcaatttt tccaagcaca agatatttgt tatccaaatc catgcccggg tcaatctcaa
    41341 atttgtaacg gtaccaggca tatccgtaaa atccccaata accttgcgac tgccagctct
    41401 gtgggacttt aatagttttc caactgctat cgtcataatt aatgtcggca tattcagttc
    41461 tgtctttaat agaaaacttc cattcgccat ccagcttata gtcgaggttt tcttcatctt
    41521 cgttatatac aagtcttacc gggccgcttg ttatacctcc atcgcgttga tcatcataaa
    41581 ctctgacagc aataatgttc tcggagttat aattgagaat gttagctggt attcggtaaa
    41641 ttcgttcgtt accatagcct gaggtataat tgggggcaaa tttgcccgtt tgcccaatta
    41701 aatggccatt gataaatatt ttgtccacat cgtcaatgtt gccaatttgc aggcaaatgt
    41761 catattttga ggctccttgt ggcaagctga acgattttcg gtaccaggca taaccgtcat
    41821 attcaacgta gccttggttt tcccagcttg aggggacttt aatctggtcc catttgctat
    41881 cgtcaaattt tactgccgac cagtcttttt cgtctccaag gctaaacagc catgtaccag
    41941 acaagttgac caatgtccgc aggtttgacg aagcagggat attggtaatg ctgcatcctg
    42001 tcagcattag tagaaataat atttttacta ttgttttcat ttcttttgtt tttacatatc
    42061 actggtggta caatgataaa actattttat atgttatttt tctgttaatt gtaaaaagat
    42121 gtaaaagttt agtttgttgg ttgccctcca ttcattgagc gcgtttgtaa catgtcctat
    42181 cagttaccta accttaaacc aaacttttat gtaatattta ccttgtttaa gggttgttgg
    42241 ctcaatatcc agtctatcta ccaccaattc taaacttctg gccatatctc tgtcttcata
    42301 gtccacattg gtaaaagaaa tcttgccttt tgtatcaacc tggaagtaaa ccaacgcaaa
    42361 cgtattatcc atttcgcggg tcatttccaa tttatcttca ataaagttgc tcactttccg
    42421 ggtgatttcg ttacttattg aattctccac agaattgctg gttacagctt cagtagcttt
    42481 tacacttacc cctaatgtta ataagaacat tacacttact aaaaaaattt ctcttgtttt
    42541 cattttctta aaatttattg ttattacatt ttgtccatga atcacgatac aaaagtatgc
    42601 cgggatatgt aagcctttag tcataaggtg taaagccatg caaccaattg taaaaatttg
    42661 taaagagtga gttgaatggt gtttttttca gaaagtattg tgtaatttcg tttccatgtt
    42721 taagaaatac acctatataa tattattcaa tgcattgctg cttccggcgg tattgtttgc
    42781 tatttttgaa ttttcggctt tgagcagcaa tgaaaaagtt attcagcagg tgtataacaa
    42841 ccagctcgaa accattgtct tttctgtcaa tcagtattct gatgacatta ttagttcctg
    42901 ggctgaaaaa atcaattact tcctgacaga atacaacaat ccggccgata ccttgagaaa
    42961 atacctgaaa agcaaggttc agttaagcaa cgtttttttg tggaaaaaca acaaggtact
    43021 ttcgttggat aaaaacatac agccccctaa aattgtttat attgaacggt taattaagat
    43081 taatgataaa caaatcagga aaaacaaaag ttatttgaat gtaaactata gaaaaaatga
    43141 gcctcttgga actgatgagg cgcaaaaaaa actttaccta gctttttggt caggcgttaa
    43201 ccctgaaagc gcccaactgt gcgtaatgga gatagatata agcaaatttg taaaagaagt
    43261 catagctgtg cggttgcagg taagtgttga gaaagaattt tatgttgcag ttttcaacag
    43321 ggctgccaat aatgatttag tatatttttc tgaatataat aaaaccccgc cggcttttaa
    43381 ttttcgcaaa aacctttggc tttttcctaa ttacgatata gccgtgcaaa taaagaaaaa
    43441 cactattgaa gatgttgtaa agtcaagact tttgttaaac atttcgctgt ttgcctttat
    43501 tgaattgtta tttgtgcttg tagctgtgtt gatattccgc tttacgcgaa aagaaatgaa
    43561 gctggctaag ctaaagtctg aatttatctc taatgtatca cacgaaatcc gcacaccgct
    43621 tgcgcttatt aatatgtaca tcgaaacgct ggacatgggg cgcatcaaac ctgaagaaaa
    43681 agtaaaagaa tattaccagg ttattttaca agaaacccaa cgtttgtcag gcattgttaa
    43741 caatatcctg aatttttcaa agttagaaag cggaaaaaca acgtttaact ttgaaataaa
    43801 ccccgtaaat tgcttggtag atgaagtgtt gagcacctat aactttcatt taaaaaacaa
    43861 aggttttgtt ttaaaaacag aactggcaga agatattttg aaagtgaagg ttgataaacg
    43921 ctcaatatgc gatgtactgg tcaatttgct tgacaatgct gttaaatata gtgcagaggt
    43981 taaagaaata actattcgta caggggttaa gtcgggttgg gtttatatag aagtggccga
    44041 taaaggaatt ggcatttcaa aagaaaacca acgctttata tttgaccagt tttatcgtgt
    44101 tacacagggt aaccttgcac accatgccaa aggaacgggt ttggggcttt ctattgtaaa
    44161 gtatgtagta gaagcccata atggcaaaat agaagttgac agtaccgaag ggaaagggag
    44221 tatatttagg gtgctgttgc ccataaaata tgatgtttaa aaaataaata ttacagatat
    44281 gcataaaata ttggtagtag aagacgaaca ggccatgttg ctcgggcttc gcgataatct
    44341 tgagattgaa ggttacgaag tggacactgc caacaacggc gctgacggat tgaataaaat
    44401 cctggaaaac gaatacaacc tgataattct ggatgtgatg cttccgcaaa tgtcggggtt
    44461 tgatgtttgc cgcacagtgc gaaaacgcgg aaagaaagtg ccaattatcc tcttaacagc
    44521 taaaggagaa gagattgata aggtatttgg cttagaaata ggggctgacg attacattac
    44581 caagccattt agcctgcgcg agctgcttgc acgtattaag gctattcttc gccgtactgc
    44641 tactatagaa agtgatgttg aacaaggtaa actaccccaa aaaattggtc gtttgcaggt
    44701 aaactttacc agctatgtag ctacggacga gactggggca gaagtgaagc tttcgcacaa
    44761 agaatttgag ctgttgcatt actttgtaaa aaacagcggc caggtaatca gccgtgacga
    44821 gctgattaaa aacgtatgga actataatta cgagatgact acccgtacca ttgataattt
    44881 tgtggtcaaa ctccgtcaaa agattgaggt aaatcctaat gaacctcatc taatccttac
    44941 agtgcatggc accgggtata aaatggtgtt gtaataaaat cttaaaacat aaccattgtc
    45001 cactccaact tttattctct taatttttca acttaataaa gtcaacacta accagcatga
    45061 cataaaaaac taaaaaaatt gtacaattat taaaaaatat tctatttttg cagcccgttg
    45121 aattttgttt ttaacggaac aaaaagcagg cgaaaacttg caaaaaatat agaaaactga
    45181 ataaaaaata agataatgaa aaaagaaatt catccgaaaa catataggct ggttgccttt
    45241 aaggatatgt cgaatgatta tactttcatt tgccgttctg ctgctgcttc taaagaaagt
    45301 attactattg aaggggttga atacccttta atcaaacttg aaatttcaaa cacttcacat
    45361 cctttctaca caggaaaaat gaagttggtt gatactgccg gtcgtgttga taagttcatg
    45421 aaccgctata aagaccactt tgacaagaaa aaaggtaaat aattaagact ttttatagaa
    45481 tcttaaaaac tccggtataa ttttatcgga gttttttgtt tataaacttt ttgacaaaat
    45541 tttcagtatg aagaatatta tactttttga tgattgcagg gttcaattgt tgccacttac
    45601 ttatacccgc cctgttgctg agcttcgtgt aggtattact acaattagtg agaaatggca
    45661 atactttttg ggagataagg ttagctttta tactgaaaaa tacctttcgg agaagtttcc
    45721 tctttgcatt gaggaagaca actatttgat aaatggaagc attattcctg atgcagctct
    45781 ggttagcgcc attgaaaaac tccaaactgc tgacgtgctt gttaaagcaa atactatcat
    45841 tgcttgccgt atgaataaag ctgctacagc gtcttttaat aatcaaatat ctgaagcagg
    45901 gaagacaata caatatagcg gagaatattt acaggttaaa aatacctggg atatttttga
    45961 attaaacgac aagttgctgc gtattgattt tcaaactctt acttctggcc ggaattctca
    46021 gccaataagc aaaacaaaca acgtcatttc tccagaaaat atatttattg aagaaggggc
    46081 aaaggttgaa tgctcaatat taaatgcatc tacagggcct atatatatag gcaaagaagc
    46141 tgaaattatg gaaggctgca tggttcgtgg ccccttagct ttgtgcgacc atagtgtact
    46201 taaaatggga gcaaagatat atggagctac aactattgga ccaggttcaa aggttggagg
    46261 agaagtacac aatagcgtat tttttgcaaa ctctaataaa gctcatgatg gatttcttgg
    46321 caattctgtt cttggcgaat ggtgcaacct gggagccgat accaataact caaacctcaa
    46381 aaataattac gctgaagtaa agttgtggaa ttatgctaca gaacgatttg taaatacagg
    46441 ccttcaattt tgtgggctaa taatgggcga ccactctaag tgtgctataa atacaatgtt
    46501 taatacaggt actgttgttg gggtaagtgc taacattttt ggtgcaggtt tcccgcgcaa
    46561 ttttatccct tcatttacat ggggtggagc acatgggctc gacacttatc cattaaacaa
    46621 agctattgaa acagcccaaa aagtgtttgg acgccgtaaa aaagactttt cagagataga
    46681 acaaaatatt tttaaacaaa tttttatcca gtccgaaaaa taccgacgtt aagcatattt
    46741 tttttggcat aactattgat actaattagc ctcctgctat aacaaacact gtatatagtt
    46801 gttgtaacaa tgaggttaaa attattttta agtaaaacgt ttgacatttt gtcataacat
    46861 aatggcacaa tatgaattat tttcaaaata gaaaaaattt cgacaatttt ttttcggaca
    46921 attacaacca tgattcgatg gaatttttcc ctatattgcc tgaagatgaa gaaaacaata
    46981 gaggcgagcc atccatgcct gagcttttgc cagtgctgcc attgcgcaac actgttttgt
    47041 tcccgggggt aataataccc attacggttg ggcgccaaaa atcaatgaaa ttgctcaaag
    47101 atgcctacaa agccaatatt atcataggag ctataagcca aaaagactca agtatagatg
    47161 aacctacaga aaaagacctt catcctatgg gcacattggc atttgtaata aaaattttag
    47221 aaatgcccaa tggcgataca actgctatta ttcagggacg caaccctttc aggcttggcg
    47281 aagttttagt taaaaaacca tatttcaaag ccacttatca ggtagttgac gacattattc
    47341 ctcctgcaaa agataaagaa tttaatgcaa ttcttgccaa tgtccgcgag ttggctatta
    47401 aaatgattaa ctattcggct aacatccccc aggaaattgg ttttgcaatt aagaatatcg
    47461 agaacccttc atttcttata cattttgttt gttccaatat taatatcagc gccaatgaca
    47521 aacagcggat gctcgaaatc cctgatttaa agcaaagggc aatgcttctg ctcgaatttc
    47581 ttaataaaga agtacaagca ttggagctca aaaacgatat acaatcgaaa gttaggcagg
    47641 agcttgacca gcagcaacgc gaatatttcc ttcagcaaca aataaaaacc attcaggatg
    47701 aactgggagg caatccgctt gaacaagaaa tagaagaact gcgcaaaaaa gcctccaaga
    47761 aaaaatggag caaaaccgta gccgaagcat ttgaaaaaga aataggcaag ttgcaacgcc
    47821 taaaccctgc tgccggcgaa tattcgttac aggtaaacta tttgcagaca ttggtagaac
    47881 tgccctggaa cgtttattct acagatgtgt ttgatttgcc taaagcacaa aaaatacttg
    47941 atgccgacca ttttgggctt gagaaagtca aagaacgtat tattgaacat cttgcagtat
    48001 taaaactaaa aggcgattta aaggctccga tactttgctt atatggccct ccgggggtcg
    48061 gaaaaacatc attgggcaaa tctattgcca aggcgttaaa aagaaaatat gtacgcattt
    48121 ctcttggcgg cttacatgat gaagccgaaa tacgaggaca tcgtaagaca tacattggcg
    48181 ctatgcccgg gcgtattttg caaaatatta aaaaagctaa aacctctaac ccggtatttg
    48241 tgctggacga gatagacaaa gtgggcaatg acttccgtgg cgacccatct gctgcgctgc
    48301 ttgaggtgct tgaccctgaa caaaacaaca atttctatga caactatctt gagttagaat
    48361 atgatttgtc taaggtaatg tttattgcta ccgccaacaa catttcggct atacacccgg
    48421 ccctgcgtga ccgtatggag atgattgaag tcagcggcta tattaccgaa gaaaaagttg
    48481 aaatagctaa aaagcacctt atccctcgcc aacttgaagc ccatgggatg aaaaaagctt
    48541 caattgccat taaaagtgag gctattgaat tcattatcga aaattatacc cgcgaatcag
    48601 gcgttcgtga tttagatcaa aagctggcaa aggtatttag gtttgttgcc cgccagatag
    48661 ctatggaaga gccttttaat aagactcttg gcattgaaga agttaaaaag attcttggac
    48721 aacctctttt cattcgtgat aaatacgaaa acaatgatta tgctggtgtt gttactgggt
    48781 tagcatggac ttcggccgga ggtgagattt tatttatcga aacaagctta agccggggac
    48841 aagaaaaaaa acttactatc accgggaacc ttggagatgt aatgaaggaa tcggccataa
    48901 ttgcccttga atatatcaag gcccatgcgg atgttctgaa tctggacatg gaaatattca
    48961 ataattggaa cgtacacatc catgtacccg aaggcgctat ccccaaagac ggcccttctg
    49021 caggtattac aatggcaacc tcattggcat cggcatttac acaacgtaag gtaaaaccaa
    49081 accttgctat gactggcgaa attacccttc gaggtaaagt attgccagta ggcggtatca
    49141 aagaaaaaat acttgctgct aaacgtgccg ggattactga cattatcctt tcttcagaaa
    49201 acagaaaaga tattgaagaa atcaatgaag tatacctgaa aggcctgaat ttccattttg
    49261 tgaaagatat tattgaagtc tataagctgg cattactgga tgaaaaggta tcgaacccgt
    49321 taaaccttat catagaaaag ccaaagaaaa aataaataat ttgaaaaggc ctttcggtat
    49381 attatccctg aaggcctttt ttattaaaag cttgatttca ttaccaagat ttggtataaa
    49441 acaaaaattt ataacaccta tattcaaaag cactgttatc cgataaaagt atctgaacta
    49501 aagtaatcca gtaaattact atttgcaacc ccagctttaa ggccggggtt agtcaaagta
    49561 atgatataat tgggctttag ccctaatatt tacaatggtg gttcaaatgg ataaattgtg
    49621 gtgatgacga aacaaatgag atgtttctaa tatttaaaaa agctaatcat ttctttcaat
    49681 tgtgcagcct gacttgccaa ttcttcagcg ctggtagcca actcttctga cgatgcagca
    49741 ttttgctgga ttatatggtt cagggcagcc aaagcattgt taatctgttc tactcccaat
    49801 gattgttcgt tgcttgccga ggcaatttct tgtaccagtt ttgcagtctt ttcaatctca
    49861 ggggtaaggc taagtataat cctctctgat tcttcagtaa ccccaatact actttgcgat
    49921 atttgagcaa tttcatctgc tgcagcttta ctcttttcgg ctaatttgcg tacctcagca
    49981 gctacaacag caaaaccttt gccatgctcg cctgcacggg ctgcctcaac agcagcattt
    50041 aaagcaagta tattggtctg aaacgctata tcattgataa tagtaatttt ccctgcaatg
    50101 tcttttattg aaacaatact tttttctcct gcatttttca tttgctccat ccctttttga
    50161 gcatgcaacg aaatcttttc agtctggaaa gcattgtctt tattttgcat aatattggcc
    50221 gacatttgtt ccattgaaga ggatacttcc tctgctgaag ctgcctgggt attagcccct
    50281 tgcgacaata actgcgaccc ctcgcttatt tgctggcttg ccattaatat ttcggatgcg
    50341 cccctgtcaa tacctccaag caattctctt agtttttctg ccatttgcga aagtgatgat
    50401 gccaatacgc ctatctcatc tttttggtca atatctaatg tagctgtcaa attacccttg
    50461 gctatttgcc tggcaaaatt tacccctttg tttattgagt ttgaaatatt ccggcttaga
    50521 tatatattga tggaaacaat aactcctata cttagtagga tagctaatac aattatgtac
    50581 cgtaaatgat aaagaatttt catcatttca tcttcataaa atgacacagc aacataagac
    50641 tctatttctt tgatatattt gaaataaagt actttatctt ttccctccca ggtgtaactt
    50701 atttttccgg tcccggaatc tgatgcaagc atttttttga aaaaatcttc gctgctcaat
    50761 atagcccctt cttttgaagg atgaacaata agtttcccgt ctttgtcaat gataaaagga
    50821 tatccggttt gtaaatattt cttctggtca aaaatagatt ttatattttt aaaatccttt
    50881 tctggtatcc ccacaaaaag catcccggct accttattat ttactattaa tggtttatat
    50941 gaagtaaagt accagtcatc tactacccaa gccctgccat tataatcttc tttcttttct
    51001 atggccagaa caactggcga attattagga atatatgtat ttatagccct atctccatta
    51061 gcttttaata ctgaagtaga aattcttaaa tacccgtttt caattttttg aaaaatagtg
    51121 gctttagcat ctgtaaggta tgtaatcttg tccaccaggt caaagttttt ataaataggc
    51181 tttttattta aaattaaagc cggaatggtt atatttgaag tgctttggct tgtttgtccg
    51241 gtaacattgg cttcaatttt ttgtttttca tcaaacgata attctccctg gttttttagt
    51301 atctcatggg ccactttcat ggccgactcg attctcatct gtctttcttt tagctgtaat
    51361 tgtactatct ggcaaagatc atccaattgc tctgtcatgc gaatatctgt gtcttcaata
    51421 attttgctac tttgcagctg ataaatataa aaacccaaca tagtcagtat tacaactact
    51481 gcagaactaa ttaggacact aatacgtaca cctattttta agctatttaa aaatttcatg
    51541 tgtttgtttt ttgaggtttt gtgatacgca aatttaaaaa agttccgtta tttttaacat
    51601 gtatttaaaa aatctacact ttcccatgtg cgataaataa gaattgaagg caaaaggtag
    51661 ctatcatacc gttaatcccc aattaagcat taggcaatat ttgccaaact atatgattta
    51721 gttgggtcta atcgcttaag tatatactaa taattaatca ttaaatatca gggctaaagt
    51781 ccgtcaggaa gtaagagttt gtacccccca accttaaggc tgggggtagt caaagctatg
    51841 aagtaattgg gctttagccc taatgcttaa ttttatatcg gacaatgttg ataattaatt
    51901 aagggttaga gtaggtataa aaaatgagtt acaaaacaaa accatgtaac tcattttctt
    51961 aacaattatt tactaacagt gtaaatgtaa atatcctctg gatattaacc cataaaataa
    52021 ctaataagca ggaataatcc gccagacaat attattgtta ccggcaaggt tactaaccag
    52081 gctatcagaa tattttttaa cgtctttttc ctcaagtttt tcaaaccatt ggttgccacc
    52141 atagaccctg ccacacccga tgacagaata tgagtagtac ttacaggtaa tttcaatgag
    52201 gttgaaaaaa atattgtact ggctgcaaca agttcagccg aagctccctg ggcataagaa
    52261 aggtgttctt tgccaatttt ttcgccaatg gtaactacta ttcgcttcca tccaaccata
    52321 gtaccaatac ccaatgataa cgatataagg agtattaccc accaaggagc atattcggta
    52381 aaagatttca tatcagcaat actggcattg agttgttttt tctgagctgg ttctaattta
    52441 atgtggatgt aatcttcatt agggtctgtt atgtttttca atagtttggc cgaaatcagg
    52501 atattttttc ttaatg
//
LOCUS       DAOSPF010000018        52105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_690_length_52105_cov_8.469414, whole genome shotgun sequence.
ACCESSION   DAOSPF010000018 DAOSPF010000000
VERSION     DAOSPF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 52105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_52105_cov_8.469414"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..403)
                     /locus_tag="PK252_05335"
     CDS             complement(59..403)
                     /locus_tag="PK252_05335"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOT14168.1"
                     /translation="MKLLFDQNISYRIVNKLIDKFPESKHVSQVGLNDTEDIDIWQYA
                     RKENYVIVTFDSDYYDISLINGCHLKVFWLRTGNLTTNEIKKLLISNSEIIHDFIKSE
                     ENADKTCLELNE"
     gene            complement(403..624)
                     /locus_tag="PK252_05340"
     CDS             complement(403..624)
                     /locus_tag="PK252_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOT14169.1"
                     /translation="MINYHSIIKRNPNKRFGKPCVRETRITVYDVLGWLAAGMTNEEI
                     IEDFPELTNEDIQACLAFAADREHKLQYA"
     gene            complement(903..2354)
                     /locus_tag="PK252_05345"
     CDS             complement(903..2354)
                     /locus_tag="PK252_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT14170.1"
                     /translation="MIKIKNTVMGTTALLFMLVSTTLMAQIGKTFIHDPSTIMECDGK
                     YYTFGTGGGGLISEDGWTWNSGAERPGGGAAPDAIKIGDRYLIAYGSTGGGLGGGHNG
                     KINTMWNKTLDPKSPDFKFTEPIVVATSEGMEDCDAIDPGLLLDPTDGRLWCSYGTYF
                     GFIRLVELDPKTGKRIEGNKAIDIAIDCEATDLEYHDGWYYLFGTHGTCCDGANSTYN
                     IVVGRSKKVTGPYLDNMGRDMLKGGGKMVVAAGERVNGPGHFGRFIVENGVEKMSCHY
                     EADLDRGGLSVLAIRPLLWKNGWPVAGENIKEGTYEIESERRGYALELIVDFVRMPVA
                     HGWRRNPNEPVKPVPSQQLADVINTWPTGNIGVRIGDYMARPHQKWTITSAPDSSGYL
                     GGPYYKIVIAGTNRALAATADAEVTTVPQFTGAPEQLWRIDQLTDGTYRIMPKVVPNS
                     NRKLALVSSGDSTPTLAPFDMDSDNSKWNFKAH"
     gene            complement(2387..3121)
                     /locus_tag="PK252_05350"
     CDS             complement(2387..3121)
                     /locus_tag="PK252_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT14171.1"
                     /translation="MNIKHKTFLVAGFVIIASINLHAQTGPAGRQMDDPALTDTTGRT
                     ISGYFNPASKTKMAPDADGFIQRWLLLEPISMPIRSNTIFTDSYLRKTFDTLYFQNQF
                     TILPKDGQKVKVAGKELAWHALDSKLWNVKLFRFAFGLKKPIYGVMFWAVTVVNCPDE
                     MKNVRLAVGSNSASMWWVNGKEALLLSGDRRMVMDDGTSNRITLNKGKNIIRGVVING
                     PGMSDFCVRFLDENGQPVKNITITLE"
     gene            complement(3387..4118)
                     /locus_tag="PK252_05355"
     CDS             complement(3387..4118)
                     /locus_tag="PK252_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14172.1"
                     /translation="MKLFFLLLCLAVFVPVIRGQETNKREMSDMPLKLDKNALNSILG
                     PAFNNNNYPGYKLNFNTLTDSAMYCDIPDSVKNKSEEYQRLYRLFGSYSDFKRKFLAL
                     KVDDKVPELGLTGSKYLTVQALNPYYSDRVVVIMDGPVSFFYKKFNKHGQAEQKYADL
                     KKYEPKQKEINRKYNTQNVQRWTGLQGQKLTKFVLYCHFSDEYLLKATEYQLIEAVFA
                     KLKEFNALADTSTNNTPEADTSKRD"
     gene            complement(4688..5398)
                     /locus_tag="PK252_05360"
     CDS             complement(4688..5398)
                     /locus_tag="PK252_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14173.1"
                     /translation="MNKTLLIASVVFCMIFTPAVSQQDSIKKLTRDSLVISILKHRSV
                     IFNFVNHTKLQPKEIQKGLETALKNVFTPEEIVNMAYDPKISRYDFDLLMENGVRAMR
                     AILNDDIIKYNNVPKTMLDDAKYESDKKAAKDTFEMNLKNESAEWYDFYVVKNGVTDD
                     WNRDRRMLNDILNSKSYITYRYALDDARRRYEAPYFKLHQDLTKLRASLLAYNVAAIA
                     LFNSYKPKGEVFRSTSNY"
     gene            complement(5616..6851)
                     /locus_tag="PK252_05365"
     CDS             complement(5616..6851)
                     /locus_tag="PK252_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT14174.1"
                     /translation="MKNSRKKYTSFCGLLLVIFLTGIANVGAKAPAKKSEKWVGTWST
                     APMLVEPANMPPAPGLNNNTIRQIVRVSIGGECLKLKFSNEFGNSAVTLKKVQIAVSK
                     GEGLIDTCTIKELSFDGKAEVAINPGTAIASDEVLFHLKPRMDVAITIYFGETSKDLT
                     GHPGSRTTSYIFAGNKVSSTDTSGVVKTDHWYIISGIDVKAPKTAASVAILGNSITDG
                     RGSVTNKQNRWPDVLSERLLANSKTQQIGVLNLGIGGNCVLKNCLGPAAVDRFERDIL
                     RQQGVRWLIILEGVNDLGQAPDSLAAVKTANDLISAYKQMIEKAHANNIKVFGATILP
                     INKSFYYTSYREAARKMVNDWIRSGQYFDAVIDFDKVMQNPEDISTLLPDLHMGDFLH
                     PNEKGYQKMGIAVDLKLFE"
     gene            7070..7441
                     /locus_tag="PK252_05370"
     CDS             7070..7441
                     /locus_tag="PK252_05370"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14175.1"
                     /translation="MEDLIIEKGANTPAITFMANGVLRIEGRSIPENPAAFYEKAFEW
                     LNDYKTTNPKKIELHVDLEYFNSSSSIILMNIFKTLRGILNHGADVRIHWHNSANDEE
                     MIESGRYYESILKMPFFYEMN"
     gene            complement(7545..8252)
                     /locus_tag="PK252_05375"
     CDS             complement(7545..8252)
                     /locus_tag="PK252_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOT14176.1"
                     /translation="MENFLQNLRNDLHSKKDETFQAISRRFFKEEIKCYGIRASVITK
                     MSKALSPQIKERPKKEIFALCEGLWKSGYLEETFFAANWAYAIRKTFEPDDFKTFKRW
                     IDNYIYNWASCDSFCNHTMGAFIEMYPSFLKDLKKLAVSENRWTRRAAAVSLIIPAKK
                     GLFLEDIFEICNILLPDKDDMVQKGYGWLLKAASHKHPIDVFDYVMSKKETMPRTAFR
                     YAIEKLPKDLRQKAMGK"
     gene            complement(8305..9492)
                     /locus_tag="PK252_05380"
     CDS             complement(8305..9492)
                     /locus_tag="PK252_05380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT14177.1"
                     /translation="MDFKHLSLFKPNMEYRRFGKTNKNISVITLGGMRYKHGWDEPRN
                     VVADDTIEECKDTVEKALANGINLIETAYGYMKSETTYGIVLNDVLKLKRGSYYLMTK
                     GAAFTADEMRRLVEEQLQALKTDYFDFYAWHGINNWDRYEKACAKGGPVEELLKMKNE
                     GLIKHVGFSTHALQEIIIKAIETDLFEFVNLHYYYFFQRNKGAIDLAQSKDMGVFIIS
                     PNDKGGQLFNPPQKLIELTSPLTPIQFNARFCLSNPAIHTLSFGFPATSPFDQIEGIF
                     PTSIPLSEQDAAIKYNLDKQRLLDKYADFDGYCMANDPSGINIPEILRFRTMLKCYDM
                     RTFGQYRYNMFVENDVWFEGAFPTEEKLAKVDLSKCPPSVPILDLLRETHAELYKAPK
                     KKE"
     gene            9861..10190
                     /locus_tag="PK252_05385"
     CDS             9861..10190
                     /locus_tag="PK252_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOT14178.1"
                     /translation="MNIFVAQLNFKIQSESLKQIFEEYGAVTSAKVINDQVTGRSKGY
                     GFVEMADDDQALHAIEELNGAEVEGKTIVVKKANPRPEGGAGNGGGRRDFRSGGGRPQ
                     RRFESRN"
     gene            complement(10260..11225)
                     /locus_tag="PK252_05390"
     CDS             complement(10260..11225)
                     /locus_tag="PK252_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT14179.1"
                     /translation="MQEKKIIPPVDPQLIEKELTEDKFIRKTNYGGNLLYTFTHQDSP
                     NLMREVGRLRELSFRDAGGGTGEEMDIDEFDISDDPYHQLIVWDPQHREILGGYRFYI
                     PHPGASGDDIAKRLSTSHLFKFSDKFKTEYMPYMIELGRSFVQPAYQSTSRARKGIYA
                     LDNLWDGLGAILVHNSNTKYFFGKVTMYTHFNKEARDLILYFLKMHFGDKDNLVSPID
                     PLIPNWDLEEMAKTFNAENYKENYKILSRRVRSLHENIPPLINAYMNLSPSMKTFGTI
                     INHEFGGVEETGILITVADLYPEKLQRHVSTYKRVRYFLRKDRMF"
     gene            complement(11257..12066)
                     /locus_tag="PK252_05395"
     CDS             complement(11257..12066)
                     /locus_tag="PK252_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT14180.1"
                     /translation="MNINVRKVFADKNPALARRLPGFFYRWLERTIHQEEMNYFLDIS
                     ANKTGIAFLDEAIAYFDLHIQTIGLEHIANDRRYIFASNHPLGGLDGIVLINILSKKF
                     GNIKAPVNDILMNIENIKEFFIPINKHGKQDRASAIETEEIYKSDKQVLTFPAGLCSR
                     KIKGKIVDLQWKKNFITKAIQYKRDIVPVYFDGKNSDFFYNLSNLRKKIGVKANIEML
                     YLPDEMFKQKGKELNIYFGKPISYETFDKSKTPEQWADFVKSIVYQSKNII"
     gene            complement(12072..13892)
                     /locus_tag="PK252_05400"
     CDS             complement(12072..13892)
                     /locus_tag="PK252_05400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT14181.1"
                     /translation="MEVPKIPMEDFFKNPEKAAFQLSPDGLYFSFTAPYKGRMNIFVQ
                     KIGSADTIRITSETDRDIRSYFWANNKRILYLKDNGGDENFRLYGVDIDASNPMCYTN
                     FEGVRTQVIDDLEDIEDEVIIAMNKRDNKVFDPYRLHLSTGKMEMLAENPGNIQDWLT
                     DHNGKLRIAVTADGTNTSILYRENENDSFQTIITTDFKESVDPLFFTFDNSMFYAASN
                     KGRDTKAIVVFDPKSKKEKKVLYSDPEYDVYGLSYSRKRKVLSAISYTTWKRKLVFLD
                     NEVKQLYQKFEKHLGTSYEISITSVNKDEDKFIVRTYSDRSLGSYYFYDLKKDSIAKI
                     AELSPWLNENFLAEMKPITYKSRDGLTIHGYLTLPVGKKAENLPVIINPHGGPWARDR
                     WGYDSEVQFLANRGYAVLQMNFRGSTGYGRKFWEASFKQWGKTMQNDITDGVQWLIDQ
                     KIADPKRVAIYGGSYGGYAVLAGLCFTPDVYCCGIDYVGVSNLFTLLKTIPPYWKPIL
                     DMMYEQIGHPEKDSAYYREVSPVFHADKIKAPLFIAQGANDPRVNKNESDQMVDAMRK
                     RGVEVEYMVKDNEGHGFGNEENRFDFYRAMEKFLEKYMKK"
     gene            14103..15821
                     /locus_tag="PK252_05405"
     CDS             14103..15821
                     /locus_tag="PK252_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14182.1"
                     /translation="MYTIKPLCLLVFAITLISCEYEPKGSFYQEIDKDIKNPEVETRS
                     LTFNKDTIVIEEGNLLGYSFFNADKKIRRILFKIDGQVFVDASTFSGSFSLKGVSKGI
                     HELEAVFMLNSGTGSIMDKLGGELYATKKTWVLVVLKAHSETINATSENGFLKIYWDT
                     CYDFDFQYFEIFKGPTLIGKTKLPFYVDSSYIGESVTYTINANRANSSTIWCSKDFKE
                     LHYDFLFISDHKRCVIKWNRPICYNAIEKTIIYINNSTFETTDTSIILPVTENGQRFY
                     FREWEVSPKYGNNKYRVFEPNTLYFYSSYKFCQSDIVNLLKIDESNFLYSSSSDKDFF
                     EYSINDKALIASQHYLDEIKLVSQSNKCIYGKLNGQDAIQSLDGNQNLLMIDGSPNII
                     ALSDYSFIRYDLDGCYLVKFGDENKVFLSQNYLENCQMSFDGKYIMFSEGGLLKINKI
                     ENDRLILKGSFENNDVRFVRFGFKYPSYLYYWNGKRFLVKNCYNFALSSDILLNTPNT
                     EILDINEQKNCILVKVNSSIHIINYTNGQILVNMELGDDARYEKLILMDNAIVSEGGY
                     IYHVNY"
     gene            15893..16612
                     /locus_tag="PK252_05410"
     CDS             15893..16612
                     /locus_tag="PK252_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14183.1"
                     /translation="MKKTFLLVAILLIFSSLHSQNLMFGLKSGVGSYSMKDMKDFNRY
                     FVSNLSFANKLTDDFPANYYFQPEVLFNYQHLTFGYSFAFYSTGSRIASNDYSANFNM
                     DDVLNCYSHGFIFEYVFYRASLFDFSVYSRFSFENTKLKITNNLMVVAEDTLLQTKYS
                     YRTKSNSTEPGIAISYDYKFLKIKYYAGYMFSIMTDGFTSDDEKKQKVYNPYTRNQLK
                     PDWSGFRTGISVYFMFSLKRN"
     gene            17150..19186
                     /locus_tag="PK252_05415"
     CDS             17150..19186
                     /locus_tag="PK252_05415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT14184.1"
                     /translation="MNENIIQKFRDKFIDESVGLLDQMEKDLLELEKAPQDKELLESA
                     FRAMHTIKGVSGMYGFEHICEFTHILENIYQSLREKKVFFNKEISDLSFAAIDHIRRL
                     LEDEKLRDEENKKRHKKLTDEINELAKRCNLSQPQPVFKQNEKVQAEKTKNSWYIILN
                     TNEQMFFRGINLTSLFKELSQLGEYHIQPIPVLTTHESDSWGIVLVSDCSENDIRDVF
                     IYIEDDIQIFRITSSDIFHPENEPKVEFEKTEQEEISSIIDLVEETEKPPVFYAEEKK
                     ESKETEIIKSASFKLQTKRIAVDSAKLDHLMYLVSELITINSQLVQATKGSYYASLQH
                     YVEQVDSLSKQFRNNALEIRLVPLSDLALRFQRLIRDLSSNLGKSIAFVTEGLETELD
                     KSTIDILAEPLMHLIRNCIDHGIESPEKRAGKGKPEKGTIKLSAVQSGSYILISISDD
                     GNGIDTEKVKQKAIEKGIIGPNDNPSDKELFDFIFLPGFSTAKSLTDVSGRGVGMDVV
                     KKKITDLRGEIIVESQKNVGTTFTLKIQQSIAIIDTLLFRVENSSLIVPMSDIEICIQ
                     MPIEELDARRNTETIEFNERLIKFYDLRQMLSLTGQYKENVKLLIIKREEQFVAIMAD
                     KIIGEHQAVLKPLGNTLKDQNIFSAASQLGDGNLAFLLDTSALFKNINILMDSF"
     gene            19252..19962
                     /locus_tag="PK252_05420"
     CDS             19252..19962
                     /locus_tag="PK252_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14185.1"
                     /translation="MKKLIAILLCMYQVHAICQEKAENSAASFSLNADVVSRYVWRGL
                     LYSPNVNVQPYAAFSYKGLSLSTWASYGISDRYAEVDFTLSYAIDNLSVSVNDYYNEF
                     EDSLGTNRHFNFKDNTTPHALEATLSYKISETMPLTLSLSSFFYGNDKDSEGENMYSS
                     YIEASYPFDFSNYSFNLFIGGTLDKGLYADKAAIVNVGFSATRQIKVSDSFQLPFSAS
                     LISNPYAEDIFMVLKLSF"
     gene            20080..22191
                     /locus_tag="PK252_05425"
     CDS             20080..22191
                     /locus_tag="PK252_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14186.1"
                     /translation="MKTQKFVSLKQKLGLIVGTGITLTALVLISYSSIKTRTDSINSA
                     KSNALALAQDFAVNISLELETAISSSRSMANALSAVGDPKSPIRLSREEAMKMAEKVL
                     FSNPNFLGFTLAFEPNAFDGQDAKFRNAEAHDKTGRFLVYQTKKVDNTVAREVLIDYD
                     DPEKAPWYWVPKKTMNEFLTEPVIYPVQGVDVLMVSFMTPVINNGRFLGNTGIDYPID
                     FMQHKVKKAGYYDSLAIISIISNEGTFVAHSKTPELINHSLNTIYSDAPGQILKIKKG
                     EKQIFVQNDTLSIHVPLNIGQTHRPWQVRMEVPMKIITAEANEQMWFQLSMGVILIIV
                     SIVVIRLFVKRIIKPVGIMSEKAKAAAEGNLVYETIGDKTNDEIGLLSDALDTMIEKI
                     KDVVSNVIISSENFVESSKQLSASAQQISSGANEQAAASEEISSSIEEMASSVNQNAE
                     NAQQTERLASSAAQNIKTANDAVIETITAMKTIIQKISIIKEIAEKTDLLAVNAAIES
                     ARAGEYGKGFAVVASEVRKLAEHSQNAAKEIDDISVSSLQIAEKSGQMLNAVIPEIQN
                     TAKLVQEITATSIEQNGGVSQISNAIQQLSTVVQQNSALSEELAASSEELTGQATLLL
                     ESISYFKISKNDISNETDEEIENQIKKLSALLDSRKKGPNKKETQIKQKVSEPKKDPL
                     KGFKIHLDDKENDKDKEYESM"
     gene            22208..22687
                     /locus_tag="PK252_05430"
     CDS             22208..22687
                     /locus_tag="PK252_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14187.1"
                     /translation="MSNTYLSFVVGEEQFAVNVTKVLEVLQKQTITKVPNAPDFIKGI
                     INFRGEVVPVFEMRVKFNLAERDETKGYVIIILDLQSGEETIRIGAIADKVRDVITLN
                     DSDIKPVPAMNKDFNTEFLQGIVKLNDKFILLLNVDKVFTSNQLQQLHDSSEVLKNA"
     gene            22717..24465
                     /locus_tag="PK252_05435"
     CDS             22717..24465
                     /locus_tag="PK252_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14188.1"
                     /translation="MKTLNNFSIGSRLTFVFGLIIVLSIVNLFVNLRGLDKSSKAIDI
                     MHVSQTSISYLLEADRDAYQSSIGISQCLDKEPNDNAELEAYITDILDNRGQIKTRYT
                     KFAEEFNVKQNQAYAAIDSNFWLKYNDLCTYSDEIVNHLKARKYKQAAAIYFTDYQDA
                     FKPMREAINQFTEIHQNDSQKSYETNKEVSRNIRINSISIFIAIVIIFLISDILLTQS
                     IAKPLSNAVNITTKISGGDLTEEFEVVGKDETSKVLGAIKAMSGKISETVSSIKISAE
                     NFFESSKQISASSQQIASGANQQASASEEISSSIEEMAASINQNTDNAKQTEKLALQA
                     VENLKIANDSVAHTLKDMKMIIQKISVIKEIAEKTDLLAVNAAIEAARAGETGKGFAV
                     VATEVRKLAEHSQQAAKEIDDLSASSVLIADKSGQLLASVTPEIQNIAKLIQEISATS
                     IEQNTGAAQISASVQQLSQVVQQNAALAEELAASSEELTGQASSLLDNVSYFKTSREE
                     IDKYTDEEFNMLMKRINEILAAKNRNGGAEPAPFQKNLKAEKNVVASVKPAAKPAPKD
                     GLDIKLEEKDPDYDKF"
     gene            24714..25997
                     /locus_tag="PK252_05440"
     CDS             24714..25997
                     /locus_tag="PK252_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14189.1"
                     /translation="MKKNIISLASLAVMLSLGQAEAQETQAKSGTDTLAPMVKEVQAD
                     LKKLKKIKISGYIQGQYQKAESKGIETFSGDDFPSDIDQRFVTRRGRLKTTYTGKLSE
                     MAIQLDVTVEKGVALKEAYYKVTEPVLKTVSLQFGLFMRPFSHELNYSNTIMEYPERT
                     RVVQNVLSGETDLGACLIFQLPKENPLSALRLDFGLFNGTSNKGADYDNEKDFIGRLS
                     YNNTFADKYITLGAGISYFNGGTWNFATNYYEWKDTAFTSITADSLKVLKKNYMGADI
                     QLTLDNSFGQTQIRGEMMWGTQPTANDKTKTFLEAYKKPAYMRDFSGGYVCLVQTIAK
                     TKHQFVVKYDWYDPNTKVNKDEVGAKTPSGATNLTTKADIAYTTLGLGWIYNWDENVK
                     ITAYYDMVSNEKTLLKGYTEDLKDNVFTLRVQYKF"
     gene            complement(26190..26810)
                     /locus_tag="PK252_05445"
     CDS             complement(26190..26810)
                     /locus_tag="PK252_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14190.1"
                     /translation="MEEMVANIQQTADNAQQTEKIAHSISQGIQKVGDSSRESLSSIK
                     VIASKISIINDIAFQTNILALNAAVEAARAGENGKGFAVVAAEVRKLAERSKIAADEI
                     VSLANQSVNVTENSAQLLEAIIADIVKTASLVQEITTASVEQNTGAVQINNAIQQLNN
                     HTQQNASSSEEMAVGAEKLNQLASQLTETISYFKLNEKDKYLNSYN"
     gene            complement(26841..27344)
                     /locus_tag="PK252_05450"
     CDS             complement(26841..27344)
                     /locus_tag="PK252_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14191.1"
                     /translation="MNFFISNLISIAAAGLAAVLLLNRFFKNSVFVRVGIVWLFNLLF
                     IMFMVGLKYKFFEDNKPVVIAITLSNIIVSVICFYYGSIAVVKPLSKSVEKLEEISNG
                     NLDVEIDTKDVNPNRDLGKLIIASEKIKQNLTKVVTEINNNVKNLNNSSRQLQLGSQQ
                     LSVSASN"
     gene            27647..29512
                     /locus_tag="PK252_05455"
     CDS             27647..29512
                     /locus_tag="PK252_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT14192.1"
                     /translation="MRILENKQLANGLASLLIVAFSFSNTTKAQPSGGPYGPIKQTYE
                     IPKTTGVIYYVAPDGKKEEQGLSLDKPTTIESAIQRAKTGDVIIVRGGIYRTGSLLLN
                     QGITIQPYADEHPVFKGTYVAKEWKDLKNGTWSVKWEHLFPSKPDTWWNRAKYIRETP
                     LHRFNNDMVFVNGKFLQSVGQENEVDKNTFYVDYEAGMVYIGIDPTNKLVEITAFDRA
                     IHRVTTECNGKTSDKKGFTLKGIDFMQYAYHGIEVDGKDPEAVSNEADHGKDVVGTLI
                     ENCSITFCGRVNVFLKGDKITLRHCKISESSTEGLYIVASNDVLLEKNIFSRNNIENI
                     TGYYPAAVKIFNQSYRVTCRDNLVTDLANSNGIWYDVGNVDGCFINNWVENVGDGSIK
                     FNKNKTWPSQNGFFFEISKGVTCAGNVFFNCDQGIFILNSCGAKVYNNTFVNSTATFG
                     RNERNPANDGTFGWHSGTGPDVDKRTGHVFSNNLMFSDARHERPMMFIWQPDTFYKRN
                     YKPQLEKLENNVMVKIPSLDTIVAIYWTQAVDKSCLMAFQNFDSLKKTYPEVKDNNRF
                     YTDINVFKSVELKQLTVLKNFPGNIDAGLVPANISALMNLPKKDYKFVGAYPPIK"
     gene            29611..32325
                     /gene="galB"
                     /locus_tag="PK252_05460"
     CDS             29611..32325
                     /gene="galB"
                     /locus_tag="PK252_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HOT14193.1"
                     /translation="MNRNVFLACIIFCIWRSVTAQHLLSRQRILINEGWRFYKYDSAS
                     QADNLIYDVRPEIKDNTESRVADAKPTEAVETKSSQNVLKAWVLPSANAFIKDPQKRH
                     LRPDGNPGSDFRFVQNNFDDNNWEHIDLPHDWAIKGPFQKGWDSEVGGGMGRLPVNGV
                     AWYRKKLDITASDKGKTIILEVEGAMSYAIVWLNGSLVGGWPYGYNSWQLDLTPYVVP
                     GGENQLAIRIDNPNYSARWYPGAGIYRNVWLIKTNPVHVGQWGTYITTKNVSKTSATI
                     NLAVIIDNNTTKEAEVEATTQIFALDSLLHKTGNAVAVFSPIKTKIRPKESSKVEAVI
                     ELNAPRLWGPLPTQQPNLYVALTTLFQNGKTIDQYETRFGIRSVEFDPDKGIKVNGER
                     IQIKGVNQHHDLGALGAAFNRRAAQRQLQILQEMGCNAIRMAHNPPAPELLELTDQMG
                     LLVIDEIFDSWERKKTPYDFHLIFPDWSEPDARAFVRRDRNCPSVIIWSFGNEVGEQY
                     TAEEGAAVGKRLYNIIKEEDPTRPTITAMNYAKPDMALPAVADMIGLNYQGEGIRQAP
                     EFEGTERIRTAPQYDAFHTRYPGKVVLSTETASAASSRGIYLFPVTKETSAPIRDGRG
                     GDSKICQVSAYELHAVDFGSSADKVFGTIERRSFVAGEFVWNGWDYIGEPTPYYEARS
                     SYTGIIDLAGFKKDRFFLYQAHWRPDYPMAHILPHWNWPARVGEVTPVHIFTSGDEAE
                     LFLNGKSLGKQKKAQYEYRLRWDSVIYEPGELKAIAYKNGIRWAEDIFSTTETASKIE
                     LTADRATILADGKDLSFVTVRVTDKNGLTVPDASNLVKFFVEGPGEIVATDNGNAMDL
                     VAFPSTARNAFSGLCLVIIRGYKGQTGTIKLIAQAEGLISASLEINIK"
     gene            complement(32426..33592)
                     /locus_tag="PK252_05465"
     CDS             complement(32426..33592)
                     /locus_tag="PK252_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14194.1"
                     /translation="MKKLIFIAAALLTMHISQAQQALWGGANIISPEIKNDNTVTFRF
                     VAPNAKEVKISGEWMPAENWQPGSTLMTKGDSGVWTYTTAALTSDLYSYYFIVDGLKC
                     SDPNNVYQIRDVATVANVFIVGNGKGDLYKVNKVPHGSVTRRWYDSPSLGLTRRITIY
                     TPPGYETSKQSYPVLYLLHGMGGDEEAWIALGRASQILDNLIAQGKAKPMIVVMPNGN
                     VVQEAAPGESSLGMYKPNFFLPNSMDGKFETSFKDIIKFVESSYRVKAEKGSRAICGL
                     SMGGFHSLHVSRTYPNTFDYVGLFSAAVNPLAKEPIPFYSDIDVTLKKQKENGCKLYW
                     IGMGKTDFLYKDMVDYRAKLDAIGLKYTYVETEGGHVWTNWRVYLSEFVPLLFK"
     gene            complement(33643..34338)
                     /locus_tag="PK252_05470"
     CDS             complement(33643..34338)
                     /locus_tag="PK252_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT14195.1"
                     /translation="MKLSVIAIDDEPLALQLVEGYIRKTPFLELKGCFDNPLSAIEFL
                     DDNPVQLIYLDIQMPDLMGTEFARTLQAKAKIIFTTAYEKYALEGFRLEAIDYLLKPF
                     AYEEFLVASHKARKLIELEQKSANKNKIEANTEFLFIKSDYKIRRINFDDILYIEGLK
                     DYVKVVVENDPKPILSISSMKAVEAKLPESKFMRVHRSFIVNLERIQTIERSRIIFGS
                     TYIPVSDQYKDKF"
     gene            complement(34335..35396)
                     /locus_tag="PK252_05475"
     CDS             complement(34335..35396)
                     /locus_tag="PK252_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT14196.1"
                     /translation="MNWFSDKKIAVFLHILAWATFILLPLYFIHENGGMDMFFIQRIY
                     FRTIIYILIFYINFFLLIPWLLFKGKKLQFYLILVALVASLYFVNESVNRLVFVNEQQ
                     QLNKVFDRAAREMKPPKHPWKFDIYNYLFTAVLVSGFSIGLRMNGRYIENEKKRKELE
                     KEKLSSELALLKNQVSPHFFFNTLNNIYSLTEINTKDAQDAILKLSKMMRYLLYESEK
                     EDTTLSSEIDFLKNYIELMKLRLNDKVSLNVALPAKFKDSKFPPLMFISFVENAFKHG
                     VSYREPSFIDIELNVGHEKINFVCRNSLVSKTENDAGSGIGLENIRKRLNLLFPGKHR
                     LYINQSDKAFEVELEIINI"
     gene            complement(35432..36652)
                     /locus_tag="PK252_05480"
     CDS             complement(35432..36652)
                     /locus_tag="PK252_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14197.1"
                     /translation="MNYTNLIKIAINALRRNKMRAFLTMLGIIIGVASVITMLAIGQG
                     SKKSIQDQVSSMGSNLLFVMPGGQFKGGVQMGNASSKSLTLDDIDAIRANSTAISRVS
                     PEVRTSGQVIYGNKNTPTTIYGVNPEYLDIRKVSIKSGRIFTQAEINSSAKVCLIGKT
                     VIKNVFGENFDPTGMIIRFNSIPFKIIGILAEKGQNNFGQDQDDLLIAPYTTVQKRLL
                     AITHVQSIYISAVSEDKSALAKTEIETILRQKHKIKDGEDDDFEVRSQEELIKMFTSI
                     SDILTILLGAIAGISLLVGGIGIMNIMYVSVTERTREIGLRMSIGGRGIDILMQFLTE
                     SILLSVTGGVIGIVLGMMASTAIGTIMSWPIVVMPQAVLLAFLVCTVIGVFFGWYPAR
                     KAANLDPIDALRYE"
     gene            complement(36755..37501)
                     /locus_tag="PK252_05485"
     CDS             complement(36755..37501)
                     /locus_tag="PK252_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT14198.1"
                     /translation="MKKIIEVRNLRKDFHVGEVTVHALRGVSLEIEEGDFVAIMGASG
                     SGKSTMLNILGCLDKPTSGDYFLDGHPMAQMSRNELANLRNRKLGFVFQAYNLLPRTT
                     ALENVELPLLYNSTVKAKERKERAMYALESVGLKDRMHHMPNQMSGGQQQRVAIARSL
                     VNDPVVILADEATGNLDTRTSYEIMALFQELNNNGKTIVFVTHEPDIARFTKSTLIFK
                     DGHIIKQEKIADRFDAKQLLAEMPVTNGYE"
     gene            complement(37595..38866)
                     /locus_tag="PK252_05490"
     CDS             complement(37595..38866)
                     /locus_tag="PK252_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT14199.1"
                     /translation="MKKKTIIWVSAIAAVLILIAFAKGCKKQAANISFETTVVKKGTV
                     SNTVTATGTVQAIKTVSVGTQVSGVIDKIYVDYNSHVKTGQLLAQIDTRTLQTSLENA
                     EANMENAQAEMTLQTANFNRIKALNEKSLVSQNDYEQALYNYTRAEAALKTAKLEYNR
                     AKINLNYASITSPIDGVVLERAVDEGQTVAASFNTPTLFSIANDLTQMQVEASIDEAD
                     IGKIKEGQRVSFTVDAFSDLKFEGKVTQVRLQPVTKSNVVTYTVIVKAPNPDYKLMPG
                     MTASIKVFVEEAIDVLTVSSKALHFTPDMAVMEEYKKSVMKKKGPDKEAPKPEGMPDG
                     QTGSTKHEKPDSTAKIVWLKKGEMVFPVPVKIGIDDDMNAQVISGLKEGDEVITAINS
                     GSTVVTKGTSSSPFMPKPPSQKSGSNPPRPQ"
     gene            complement(38888..40225)
                     /locus_tag="PK252_05495"
     CDS             complement(38888..40225)
                     /locus_tag="PK252_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT14200.1"
                     /translation="MMKISWKINSLLLLALMGLPLEGQVTDSVWTLPKCIDYAMQKNI
                     SIQQKALTNETNRINANQAKAMRIPSLNAAVSQNFQWSRSFDNANSTYGKYSSSDGTN
                     YSLNSSLRLFNGFKTTNDIKQAELNYQAGNYDVETLKEAISLNVLDAYLQVLYSEEQV
                     KNSEKQIESTSHQLTLSEERLKLGSISRADYLQVNSQLATEKLTLANAQNQLAVNRVS
                     LMQLMELPVSSSFAIEHPDMEISEAFNAEYNADSVYSQALEIKPQIKSAELNKQIAEV
                     NVSIAKSGYSPTLTLNAGLSTGYISGVGMNYDYQVKNKLNPSVGLTLSIPIFQNRQVH
                     SSVAIAKIGTTKASLNETDVKNQLRKSIEQACVNLKSAYVKYQASLEKFNSAQEAYNV
                     AQEKFSLGSLSSVDFLIQKTNLITAESDLLQSKYNLVFSKKILDFYLGKPLTL"
     gene            40454..41092
                     /locus_tag="PK252_05500"
     CDS             40454..41092
                     /locus_tag="PK252_05500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT14201.1"
                     /translation="MDKYSITIDTYNKSAVSFQDKFMNMDLYNATYDKFCQKVEKKHA
                     SILEIACGPGNITKYIAAKRPDFSIFGIDLADKMIELARVNVPSARFEVMDCRDILKI
                     NNTFDAIMCGFCLPYLTKEEATKLISDAFQLLNAGGVLYISAMEGDYSKSGYETTSFS
                     NGNQVYVHYHQADFLKEKFKEAGFVDVELITQQYPEPDGSFTTDMIFFAQKP"
     gene            41226..42233
                     /locus_tag="PK252_05505"
     CDS             41226..42233
                     /locus_tag="PK252_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14202.1"
                     /translation="MKKIILLLVFLAFFSHGFSQTIHSPDEILKIMMDSKFSYEIKKL
                     EKTIECKDYSERLITHDYYRVVSDSGLFTKEITINEKAKPLFEKAEYYFQKNKLDSAI
                     TFYQLSLSADSSIYTVMTYIGQVFEKIGDNNNAILWYKKAISKNYIDYMAHWFLADAY
                     LNANNLKDAVDEIVIAQILNRNNPRIKKSFTNIFEKAKRNTDDWCFNPQIELIKESDT
                     KASVAFNEKWTGYAMAKALWTFEPGYKESMGVTNGYSTLEDKECLIALLIALENAKIS
                     INDEPQLKILKDAAMNKYLQEYIIYEIVLPQSPFVAYQLPEQTILNIKNYILNFRNTM
                     K"
     gene            42307..43086
                     /locus_tag="PK252_05510"
     CDS             42307..43086
                     /locus_tag="PK252_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT14203.1"
                     /translation="MSFEILTSELLVEFTLKVNQSPVTKLNQMKKLELPVDYFQFYNS
                     VSSVYSSKIEGEDIDFDSYFKHKFMKIQFKADYTAKADDLYSAYEFIASKKITLENVK
                     KAHAILSSNLLPKSQQGVFRTNPMFVINSDDQIEYVAASAEQVIFELEKLFRDIGILQ
                     NKELNSYEIFYFASLIHLVFVKIHPFQDGNGRTARLLEKWFLIEKLGEKAHSIQLEKN
                     YYQKLKDYYNNIKKLGLEYESLDYKKGIDFLLMTVQSINEE"
     gene            43263..43499
                     /locus_tag="PK252_05515"
     CDS             43263..43499
                     /locus_tag="PK252_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14204.1"
                     /translation="MIKIRNLTIDDLDGLIKLLQQLWVDKEINKEAVRVVIEKGLHSD
                     NQKYICATDGEKLAGYCSLTIKKQPVGIGQPRQR"
     gene            43507..43695
                     /locus_tag="PK252_05520"
     CDS             43507..43695
                     /locus_tag="PK252_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT14205.1"
                     /translation="MVDSQYRNQGIGKLLLNEIEAIARQHKCVKIELDSAFHRTAAHE
                     FYERLGFKKRAYLFIKEL"
     gene            43741..44541
                     /locus_tag="PK252_05525"
     CDS             43741..44541
                     /locus_tag="PK252_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT14206.1"
                     /translation="MTPPAIIGHQKNRTAFITLDTSKCSACWLCLSECPDKVIGKTSG
                     NMPPHAYIAFPKQCKGCLQCVKVCPTNAFTLINTLQVKKDKEKKRLITQLVAYIFLFT
                     ISLLMILSGLVLQISYHIGEQKKFHENAEKLLTPTMTYKQVRAIDATRTVVGLNYADW
                     SLVHKIAISLCTLLLMYHIFLQRKWYSMIFVHKLWGKNIGTLSLSLLFILLAITGYIS
                     WFGGFSELTRNQLIEIHDKLGILLVVLLVWHVAKRIKWFVNAYGKLYL"
     gene            complement(44875..45669)
                     /gene="lgt"
                     /locus_tag="PK252_05530"
     CDS             complement(44875..45669)
                     /gene="lgt"
                     /locus_tag="PK252_05530"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT14207.1"
                     /translation="MVLNFIQWDVNPVIFELGPFQVRWYGLLFAMAFVLGYIVFKKIF
                     NKEGHTIEMLDQLTVYLAIGTIIGARLGHVLFYDPGYYFAHPLKILAIWEGGLASHGS
                     AIGMLVALYLWARKNKQPYLWLLDRIGIVVALGGMCVRLGNLMNSEIYGIPTSMPWGF
                     IFVRENEILPKHPTQIYEALSYLAIFGFLFYSYTKEWLFNKRGLAFGIFLILLFSARF
                     LIEFIKNPQEDFEKGMPLMMGQILSIPFIVAGIILVFYALRKSAKS"
     gene            45984..46520
                     /locus_tag="PK252_05535"
     CDS             45984..46520
                     /locus_tag="PK252_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HOT14208.1"
                     /translation="MGKKNVSERWKVLKLPNLDITEKYAISDHGRVKSFKCHEKGKII
                     NCSVIRGYKAIVVKLSSGKYTSLYVHKLVAEMFLERAFLLQKYVIHKDYDKSNNHFQN
                     LCWVTFQTMNAHQKLNPNNKVLKVNHAKLSEEEVKTIKLKLKKNVKALILAKKYGITH
                     TQVYRIKNGQNWKHVNVG"
     gene            complement(46759..48078)
                     /gene="metK"
                     /locus_tag="PK252_05540"
     CDS             complement(46759..48078)
                     /gene="metK"
                     /locus_tag="PK252_05540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT14209.1"
                     /translation="MAYLFTSESVSEGHPDKVSDQISDALLDEFLKSDPNSKVACETL
                     CTTGLVVLGGEVKTEGYVDVQQVARKVIERIGYNKGEYMFESNSCGVISAIHEQSPDI
                     NMGVVRIKEEDQGAGDQGMMFGYATNETENYMPLSLDLSHLLLRELAAIRREGKEMTY
                     LRPDSKSQVTVQYDDNNKPVRIHTIVVSTQHDEFVLPKTNHPEDKHEAEKEMQMVIKN
                     DVQNILIPRIMKQLPERIKGLFKNDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYG
                     GKGAHGGGAFSGKDSSKVDRSAAYAARHIAKNMVAAGIADEVLVQLAYAIGVAQPVSL
                     FINTYGTSKVKLSDGQIAEKIAKIFDNRPFAIVKRFGLKNPIFLETASYGHFGREPFE
                     AEVEVFYKDSETYTRQTNGHEQIFKKVKYFGWESLDYVDVLKKEFGL"
     gene            48497..48940
                     /locus_tag="PK252_05545"
     CDS             48497..48940
                     /locus_tag="PK252_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT14210.1"
                     /translation="MEDLAMLALRNLNSLPSFVEEFFNHELPYSWNRNWATISTPAVN
                     IVESKDEFRIEVAAPGLTKEDFKINLENDQLTISANKEVKKEENEETYTRREFSYSSF
                     SRSFTLPETVDSEKIKASHQDGVLTIHVPKKEEAKPKPAREIAVA"
     gene            complement(48998..49252)
                     /locus_tag="PK252_05550"
     CDS             complement(48998..49252)
                     /locus_tag="PK252_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOT14211.1"
                     /translation="MSKLTTLPNIGETLAEKLEKAGIEDEKKLKVLGSEKAFLLIKEI
                     DPKASLNNLYSIEGAIQGVKSALLSKEKKDELKEFFQLCQ"
     gene            49388..50554
                     /locus_tag="PK252_05555"
     CDS             49388..50554
                     /locus_tag="PK252_05555"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOT14212.1"
                     /translation="MSNFDEIINRKNTQCLKYDALFERFGNTKLLPMWVADMDFRVSQ
                     PITEAIMNVAKHGIYGYSVESQKYYDAVKYWFENKHQWQLDDDSIFFTNGVVSAIHNL
                     INAFTSKGDGVLIQSPVYYPFFSLVKSNDRKLLVNQLIELNNQYFIDFDDFEKKAREA
                     SMFILCSPHNPVSRVWKRDELERMAQICYDNNVIIISDEIHNDLVQKPFKHIPTATIN
                     TLIEDITITCHSASKTFNLAGLNTAYIITKNRKWKQQIQQHYARQHAHAFNIFGAEAM
                     YAAYMHSDQWYSQLLQYIKDNYLYLVQYIQANIPHVKITPMEATYLIWLDFRAFNMTD
                     QQLKNFIIDNAALALNDGPSFGIGGSGFQRINIACPRKTLEIALIQLTKACNSL"
     gene            50640..51578
                     /locus_tag="PK252_05560"
     CDS             50640..51578
                     /locus_tag="PK252_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT14213.1"
                     /translation="MIFDIPNLREKRLIQWAKINRVNIPHDPAQLVNVTSLDISFKNL
                     KALPKEIGCLVNLEEMDASYNQIADLPWEFAQLKNLVRLNLSNNLFSDIPGIVCNHVQ
                     LESLNIEGNFIKKITPVIANLSSLVNFSIGYNQIFELPIEFGHLKHVVNLDLSCNALH
                     ELPSSFVKLYNLKNLKIGGNQFAQKPALLAEMPNLETVDESKDIEIINKAFLFSVISD
                     STRRVEKYIALGADVNYQLKDYQNTPFTTALFEAQSLEMVKILLAYGANAAVQRQYKM
                     TVKSFFGQKEKQVTETFLTKKHKPEIEKYLKSIEMI"
BASE COUNT        15911 a        10145 c        10234 g        15815 t
ORIGIN      
        1 acctgtgtac cctgactcaa aattgcatgg taccgaaaat attgaaaaga aaaaatattt
       61 actcgtttag ttctaagcat gttttatcag cattttcttc acttttaata aaatcatgta
      121 ttatttcaga atttgaaata agtagtttct ttatttcgtt agtagtaaga tttcctgttc
      181 ttagccaaaa tacttttaga tgacatccgt ttattaagct aatatcataa taatcggaat
      241 cgaaagttac aataacgtaa ttttcttttc ttgcatattg ccaaatatca atatcttctg
      301 tatcatttaa tcctacttga cttacatgtt ttgattcagg aaacttatca attaatttat
      361 tgacaattct ataagaaata ttttggtcaa atagtagttt cattatgcat actgcaattt
      421 atgttctctg tcagctgcaa aagccaaaca tgcttgtatg tcttcatttg ttaactctgg
      481 gaaatcctca attatttctt catttgtcat gcctgcagct aaccatccca aaacatcata
      541 aactgttatt cttgtttcac gaacacaagg ttttccaaat cttttatttg gattcctttt
      601 tattattgag tgatagttta tcatatcatc taaattttat ttatcaaata taatctattt
      661 tttatgctta taaaagtcga aagtactact ctgagatgcg agagctcatg tggcctaact
      721 attatctatc catcataatc atgggtattt cttgattatc aaaggtagtg gataaggggt
      781 aataacccca acaaaatcat atagtttggc aaaattttgg cccctgatac ttaataaaca
      841 aagaataaaa aaaggcgcag atgatggtta acccggcgcc tttcttgctg aaggatatat
      901 ttttagtgag ctttaaagtt ccattttgaa ttgtcgctat ccatatcaaa cggggcaagt
      961 gtgggagtgc tgtcgcccga agataccaat gctaactttc ggtttgaatt gggcacaacc
     1021 tttggcatta tacggtatgt gccatcggtc agctggtcga tgcgccacaa ttgctcaggg
     1081 gcacctgtaa attgggggac agtagttact tcagcatcag ctgttgctgc caaagcacgg
     1141 ttggtgcctg caattactat tttgtaatat gggcctccta aatatccgct tgagtcgggg
     1201 gctgacgtaa ttgtccattt ctgatggggg cgggccatgt aatctcctat tctgacccca
     1261 atgtttccgg ttggccaggt attaataaca tctgccagtt gctgtgatgg gacaggcttt
     1321 acaggttcgt tggggttgcg cctccagccg tgggctacgg gcattcgcac aaagtcaact
     1381 atcaattcaa gggcatatcc tcttctctcc gattcaattt cgtaagttcc ttctttgatg
     1441 ttttcgccgg caacgggcca tccatttttc cacagtaaag gacgaattgc taatacgctg
     1501 aggccgccgc ggtccaagtc tgcttcgtaa tggcaagaca ttttttcaac gccattttca
     1561 acaataaaac gcccgaaatg ccccgggccg ttaactctct caccggcggc aacaaccatc
     1621 ttgcccccgc cttttagcat atcgcgtccc atgttgtcaa gataggggcc ggttactttt
     1681 tttgaacgcc caactacaat attgtaggtt gagtttgccc cgtcgcaaca ggtaccgtgt
     1741 gtgccaaaca aataatacca tccatcgtgg tattccaggt cggtagcttc gcagtctatg
     1801 gctatatcta ttgctttatt gccttctatg cgtttgccgg ttttggggtc gagttctact
     1861 aaacgaatga acccaaaata agtgccgtac gaacaccaca aacggccatc ggtaggatcg
     1921 agaagcagcc ccggatcaat ggcatcacaa tcttccatgc cttcagatgt agcaacaaca
     1981 atgggttcgg taaatttgaa atcgggcgat tttggatcaa gtgttttgtt ccacatggta
     2041 ttaattttgc cattatgccc gccgcccaag cctccgccag ttgagccgta agcaatgagg
     2101 tagcggtcgc caatttttat tgcatcaggg gcagcgccac caccaggcct ttcggccccg
     2161 ctattccatg tccaaccatc ttcagatatt aatccgcctc ctccggtacc aaatgtgtaa
     2221 tactttccat cacattccat gatggtagac gggtcgtgta taaatgtttt ccctatttgc
     2281 gccataagtg ttgtgcttac caacatgaac aacagggctg ttgtgcccat tactgtattt
     2341 tttatcttta tcataatatc aaatttctaa attatagaca tttgttttac tcaagggtaa
     2401 tcgtgatatt ttttaccggc tggccatttt catcaagaaa gcgaacgcag aaatcgctca
     2461 tccccgggcc attgataaca acgccacgga taatgttttt ccctttgttg agtgtgatgc
     2521 ggttagatgt gccatcgtcc atcaccattc gtctgtcgcc cgaaagcaac agggcctctt
     2581 ttccgtttac ccaccacata gaagcagagt ttgatccaac ggccagcctt acatttttca
     2641 tttcatcagg gcagttaaca acggttacag cccaaaacat cacaccgtat ataggttttt
     2701 ttaacccaaa agcaaaacgg aataatttca cgttccaaag tttgctatct aatgcatgcc
     2761 aggccaactc tttgccggcc acttttactt tttggccgtc tttggggagt atggtaaact
     2821 ggttttggaa atacaaggtg tcaaaagttt ttcgcaggta gctgtcggta aaaatggtat
     2881 tactgcggat tggcatgcta atgggttcga ggaggagcca acgttggata aagccatctg
     2941 catccggggc catttttgtt ttagaagccg ggttaaagta tcccgaaatt gttcttcctg
     3001 tggtatcggt taaagccggg tcgtccattt gacgccctgc agggccggtc tgggcatgta
     3061 agtttatgct tgctataatt acaaagcccg ctaccaaaaa agttttgtgt ttgatgttca
     3121 tagtgtatta tgccgagttt atgttgttag taaaagaatc aaaaaaaaat tcctttgcct
     3181 gcaatactac gaaaaaaaat gaaaaaagca ctgcaaagct catgaacatt ctgtttgagg
     3241 ggggcaaaag tctgtttttt gacaataaat taacatttac aagctggccg ttaactttat
     3301 ctgtaagcaa gctatgcttt accaaccata gctggcagca tgatagttat agttgttaaa
     3361 gggttattac aatgttttgc aatttattaa tcgcgtttac tggtatctgc ctccggggta
     3421 ttgttagtgc ttgtatcggc aagggcatta aattctttaa gtttggcaaa aacagcttct
     3481 ataagttggt attcggttgc tttgagcaga tattcgtcgc taaagtggca ataaaggaca
     3541 aatttagtga gcttttgtcc ctgtaagcct gtccaacgtt ggacgttttg tgtattatat
     3601 tttcggttta tttctttttg ttttggttca tattttttta agtcagcata cttttgttca
     3661 gcctgtccgt gtttattgaa ttttttgtaa aagaatgaaa cagggccatc cataataact
     3721 acaaccctgt cagaatagta tgggttcaaa gcttgcacag tgaggtattt tgagcccgtc
     3781 agccccaatt cagggacctt atcatcaacc tttagtgcca gaaactttct tttaaaatcg
     3841 ctataactac caaacaaccg atacaacctt tgatattctt cagatttatt tttaaccgag
     3901 tcggggatat cacaatacat tgccgagtcg gtcaaggtgt taaagttgag cttgtaacca
     3961 ggatagttgt tattgttaaa tgccgggcca agtatgctgt ttagcgcatt tttgtccagt
     4021 ttcaaaggca tatctgacat ttcccgttta ttcgtctcct gaccacggat aacggggaca
     4081 aaaacagcca aacacagaag taaaaagaaa agtttcatag ttgggcaaag ctaagcttta
     4141 aggcttaatt aaaagtgtta atttttttga aaagaaggta ggatgaaccc cgaagggctc
     4201 tccggatgaa tttttatcct tgtttgtgtt taacgaatca tgctcgtttc atcatattta
     4261 ttttttagtg ggatgatagc ttgtcccgtc aaagacggga caacacccac aaataaatac
     4321 catcgtgctt attcacttcg ttcatgaggg cgcttcgctt agttgcgttc gcaaaaacat
     4381 gggtgtttca ataaatctaa attaagtgtc tttgtggggt aacgagagtt tgatataaga
     4441 attacattgt caattattta ataaaaaaca ataaatacat tatttaactt ttagccactt
     4501 tatggcaagc cttaaatttt tgaaacattt gtttcagggc aaaagtttgt aatgcctgta
     4561 aatatcaatt acctgagttc tttttatgcc agactcgtta aaatacttac tgctattggt
     4621 tttaagccaa gtatgtggtt gcttgtaccc ttcgataaag cttttgcttg atgaattgcg
     4681 aaaattctta ataattactt gtgctccgga atacttcacc tttaggttta taagaattga
     4741 aaagcgcaat ggctgctacg ttgtaagcaa gcaaactggc cctcaatttg gtcaggtcct
     4801 ggtgaagttt aaaatatggg gcctcatatc ttcttctggc atcatcaagg gcatacctgt
     4861 atgtgatata agacttactg tttaatatat cgttcagcat cctgcggtcg cggttccaat
     4921 catcggtcac gccatttttt acaacataaa agtcgtacca ttcggcgctt tcattcttca
     4981 ggttcatttc aaaggtatct ttagccgcct ttttatcact ttcatatttt gcatcatcta
     5041 acattgtttt gggcacgttg ttgtacttaa tgatgtcatc gttcaggata gccctcatgg
     5101 ccctgacccc attttccatg agcaagtcaa aatcgtaccg tgatattttg ggatcgtaag
     5161 ccatattgac aatctcctca ggcgtgaaca cgtttttcaa agctgtttcc aagccttttt
     5221 gtatttcttt aggctgcaat ttggtatggt ttacaaagtt gaaaataacg ctccgatgct
     5281 ttaaaatact aataactaat gaatcacggg taagcttttt tatagaatcc tgctgtgata
     5341 ctgcaggtgt aaaaatcata caaaagacaa cactggcaat aagtagagtt ttattcataa
     5401 aagtttattt tagggcaaaa gtgtttttat aatagttttt gtattgttta gtttattcaa
     5461 atttttaaaa ataaaggttt taaaaatttt aacctaaaaa ataaagttca aatttagtta
     5521 aaagcttttt tagccagttt acgtcaagcc ttaaactttt aaattttttg cttcagggca
     5581 aaatccaacc aggccaggta atgagctgta acaatttatt caaaaagctt caaatcaaca
     5641 gctatgccca ttttctgata gcccttttca ttggggtgca agaaatcgcc catgtgcagg
     5701 tcgggtaaga gggtacttat gtcttcgggg ttttgcatga ctttgtcaaa atcaatgaca
     5761 gcatcaaaat attggccgga gcggatccaa tcattgacca tttttcgggc ggcttcgcgg
     5821 tatgaagtat agtaaaaaga cttgtttatt ggcaatatgg tagccccaaa cactttgata
     5881 ttattggcat gggctttttc aatcatttgt ttgtaagccg agataaggtc gttagcagtt
     5941 tttacagctg caaggctatc aggggcctgg cctaaatcat tcaccccttc caggatgatg
     6001 agccagcgta caccttgctg tcttaaaata tctctttcaa aacggtcaac agcagccggg
     6061 cctaaacagt ttttgagaac acaattgccg cctattccaa ggttaagcac ccctatttgt
     6121 tgtgttttgc tgtttgccaa aaggcgttcg gagagtacgt ccggccaccg gttttgtttg
     6181 tttgttaccg agccgcgtcc atcagtaatc gaattgccca atatagctac agatgcagct
     6241 gtttttgggg cttttacatc aataccgctg atgatatacc aatggtctgt ttttacaacc
     6301 ccacttgtat cggtggaaga aactttgttg ccggcaaaaa tataagatgt agtgcgcgat
     6361 cctggatgcc ctgtcaggtc tttcgatgtt tctccaaagt aaatagttat agctacatcc
     6421 attcggggct ttaagtgaaa taaaacctca tctgatgcaa tggcagtgcc ggggtttata
     6481 gcaacttcag ctttaccatc aaagctcaat tctttgatgg tgcaggtatc tatcagccct
     6541 tctccttttg aaacagcaat ttgtaccttt ttcagggtaa cagcactgtt gccaaactcg
     6601 tttgagaatt tgagttttaa acattcgccg cctattgaaa cccttactat ttgtctgatg
     6661 gtattgttat taaggcccgg ggccggcggc atatttgcag gctctacgag cataggcgct
     6721 gtgctccatg tacctaccca tttttctgat tttttagcag gagcctttgc gcccacattt
     6781 gcaatacctg tcaaaaagat gactaaaagc agaccacaaa atgaggtata tttttttctt
     6841 gagtttttca tattttctta gtttttaggg tgataaaagt agtgattata ttaaaaatag
     6901 tgaagtatta atttgtgttt ttaagtaggc atattcagtc tttttagccc aagggacaat
     6961 ttgtttttag ttttatcctt gcgaaaaaga aaagcaaaaa aacgtcttaa ctttttttta
     7021 agtttttcgt aaaagactta gcgtaacact aacactaact ataattaaga tggaagattt
     7081 aataattgaa aaaggggcta atacgcctgc cattaccttc atggcaaatg gcgtgttgag
     7141 aatagaaggg aggtcaatac ccgagaatcc ggcagcattt tacgaaaaag cctttgaatg
     7201 gcttaacgat tacaaaacaa cgaaccccaa aaaaatagag cttcatgttg atttagagta
     7261 tttcaattcg agtagcagta ttattttgat gaatattttc aaaacactca ggggtatctt
     7321 aaaccacggt gcagatgttc gtattcactg gcacaatagc gctaacgacg aagagatgat
     7381 agagtctggc cgctattacg aaagtatttt gaaaatgcct ttcttttacg aaatgaatta
     7441 aaaggaaatg ctgaacacaa cgacacgaag gcacaaagta gtaataatca aatttaatcc
     7501 attgggtctt cgtgtcttta ttgtttcact tttattcaag cagattattt ccccattgct
     7561 ttttgcctca ggtcttttgg cagtttttca atagcatagc ggaaggcagt gcggggcatg
     7621 gtttcttttt ttgacattac ataatcaaac acgtctattg ggtgtttgtg gctggctgct
     7681 ttcaatagcc agccatagcc tttttgtacc atatcatctt tgtcgggcag aagtatattg
     7741 catatttcaa aaatatcttc gagaaataat ccttttttgg caggaataat aagtgatact
     7801 gcagcagccc gtcttgtcca gcgattttct gaaacagcca attttttcaa atctttcaaa
     7861 aatgacggat acatttcaat aaaagctccc atggtatggt tacagaagga atcgcacgaa
     7921 gcccaattat atatgtaatt atctatccat cgtttgaaag ttttgaaatc atcaggctca
     7981 aatgttttgc ggatggcata agcccagttg gctgcaaaaa aagtttcttc aagatagccg
     8041 gatttccaga gcccttcgca caacgcaaaa atctcttttt tggggcgctc ttttatctgt
     8101 ggggacaatg ctttgctcat ttttgtaatg acagaagccc gaatgccgta acatttaatt
     8161 tcttctttga aaaaacgccg gcttatggcc tggaatgttt catctttttt tgagtgtaga
     8221 tcgtttcgga ggttttgcaa aaaattttcc atttttttgt ttttgtatct taaaccttcc
     8281 aggttttcta tcgatttgaa ctaattattc ttttttcttt ggtgctttat aaagctcagc
     8341 gtgtgtttct cttagcaaat cgagtatggg tactgatggc gggcacttgc taaggtccac
     8401 ctttgccagt ttttcttcgg tcgggaaagc cccttcaaac caaacatcgt tttcaacaaa
     8461 catgttgtaa cgatattggc caaaagttcg catatcatag catttcaaca tagtcctgaa
     8521 acggagtatt tcggggatgt tgatacccga agggtcattg gccatgcaat agccgtcaaa
     8581 atcggcatat ttgtccagca accgctgttt atcaaggttg tatttaatag cagcatcttg
     8641 ttctgacaat gggatggagg tggggaatat gccctctatc tggtcaaaag gcgatgtggc
     8701 cggaaatccg aatgacaggg tgtgtattgc cgggtttgac aggcaaaaac gggcgttgaa
     8761 ttgtataggg gtcaacggag aagttagttc gatgagcttt tgaggggggt taaatagctg
     8821 tccccctttg tcgttaggag atataataaa tacccccatg tctttgcttt gtgctaagtc
     8881 gatagcgcct ttgttgcgtt gaaagaagta gtaataatgc aggttgacaa attcaaaaag
     8941 gtctgtttct atagctttga taatgatttc ctgcaaggca tgagtgctaa aacctacatg
     9001 tttgataagc ccttcgtttt tcattttcag caattcttct acagggccac ctttggcaca
     9061 ggctttttcg tacctgtccc agttgttgat accgtgccag gcataaaaat caaagtagtc
     9121 ggtttttagg gcctgcagtt gttcttctac caggcggcgc atttcatcgg cagtaaaggc
     9181 tgcccctttg gtcatcaggt aataagagcc acgtttgagt tttagtacat cattgagcac
     9241 aatgccatag gttgtttcgc ttttcatata gccataggct gtttcgataa ggttaatgcc
     9301 atttgctaaa gccttttcaa cagtatcttt acattcttca atagtgtcat cggctactac
     9361 attgcgtggt tcgtcccagc cgtgtttgta tcgcatccct ccgagggtaa tgacgctaat
     9421 gtttttattt gttttgccaa agcggcggta ttccatgttg ggtttgaaaa gtgaaagatg
     9481 tttgaaatcc ataagtaaaa attttttaca aaaataattt ttttaaaggt gaaagatttc
     9541 gggccagaaa aaacagttat aaaataaaat gttaaaccaa atttttaatt tacataacaa
     9601 aatgtcggta tattctgttt ttgcattgcc ctgtaaaagt ttttataatt gaaaagcttt
     9661 ttaaatgata agaaaatgaa cgaattacaa cgaaaaaaaa tgaaaatgta aaatataatg
     9721 ttatctttgc aaatgtttgg cgttaagcgc tgctttatat ttttagtttt cttattgtat
     9781 ctgaatcctg aaaatcttct gccctcttaa gccaggtaca tttaattatt taatttttta
     9841 acttttattc atcatttttt atgaacattt ttgttgcaca gctgaatttc aaaattcaga
     9901 gcgaatctct taaacaaatt tttgaagagt acggagcagt tacatcagct aaagttatca
     9961 acgaccaggt aacaggccgt tcaaaaggct atggttttgt tgaaatggct gacgatgatc
    10021 aagctcttca tgctattgaa gaactcaatg gtgcagaagt agaaggcaaa actatcgttg
    10081 ttaagaaagc taatccacgt cctgaaggtg gagctggtaa tggcggtggc aggcgcgact
    10141 tccgctcggg tggtggccgt cctcaacgca gattcgaaag ccgtaactaa gaaatttttg
    10201 ttttataatg cataaaaaac ctggtgatga caccaggttt tttttatttc gtgcgtattt
    10261 taaaacattc tgtcttttct gaggaagtaa cggacccgct tgtaagtaga aacgtgtcgt
    10321 tgaagttttt cggggtaaag gtcggccacg gtgattaaaa tgcctgtttc ttcaactcct
    10381 ccaaactcgt ggttgataat ggtcccaaag gttttcatgg aaggcgaaag gttcatataa
    10441 gcattaatga gcggggggat attttcgtgg agcgaacgta ctcgtctgga gagtatttta
    10501 tagttttctt tataattctc tgcattaaaa gtctttgcca tttcttccag gtcccaattg
    10561 gggataagag gatcgatagg gcttactaag ttgtctttat cgccaaagtg catttttagg
    10621 aagtaaagta tcaggtcgcg ggcttctttg ttgaagtggg tgtacatggt tactttgcca
    10681 aagaagtatt tggtattgct gttgtgtacc aatatagccc caagcccgtc ccacaggtta
    10741 tcaagagcat atatgccttt acgggcacgg cttgtacttt gataggcagg ctgaacaaag
    10801 gagcgcccaa gctctatcat gtaaggcatg tattcggttt tgaatttatc ggaaaatttg
    10861 aagagatgtg atgtagaaag ccttttggca atatcatctc ctgaagcgcc cggatgtggg
    10921 atataaaacc tgtaacctcc aagtatttcc ctgtgttgag ggtcccaaac aataagctgg
    10981 tgatatgggt cgtctgaaat gtcaaattcg tcaatgtcca tttcttcgcc tgtcccccct
    11041 ccggcatcgc gaaatgataa ttcgcgcagg cggcctactt cgcgcatcag gttgggcgag
    11101 tcttgatggg taaatgtata aagcaggttc cctccgtaat tggttttacg gataaactta
    11161 tcttcggtga gttccttttc aataagttgc gggtcaaccg gtggaataat ttttttctcc
    11221 tgcatcacaa aaaatttgga ctacaaaatt aatattttag atgatatttt ttgactggta
    11281 aacaatactt ttcacaaaat cagcccattg ctcaggggtt tttgacttgt caaaagtttc
    11341 ataagatatt ggtttgccaa agtaaatgtt tagttctttc cctttttgtt tgaacatttc
    11401 gtcgggcagg taaagcattt ctatattagc ttttacacca attttttttc taagattgga
    11461 caaattgtaa aagaaatcag aattttttcc gtcgaagtaa accgggacta tatcacgttt
    11521 gtattgaatg gctttggtga tgaaattttt tttccattga aggtccacaa tttttccttt
    11581 tattttgcgt gaacacaatc cggcagggaa agtaaggact tgcttgtcag acttgtaaat
    11641 ttcttctgtt tcgatggccg aggccctgtc ttgtttgcca tgtttgttta ttggtatgaa
    11701 gaattctttg atattctcaa tattcatcaa tatgtcattg acaggggctt tgatattgcc
    11761 gaactttttt gaaaggatat tgataagtac aattccatca aggcctccaa gtggatggtt
    11821 agaggcaaaa atataccgtc ggtcgttagc aatatgttca aggccaatag tttgaatgtg
    11881 caagtcaaaa taagcaatag cttcatctaa aaatgcaatg cctgttttgt tggcactaat
    11941 atcaagaaag tagttcattt cttcttggtg gatggtgcgt tctaaccatc tgtaaaagaa
    12001 ccccggcaaa cgacgggcca aagccgggtt tttatcggca aaaaccttac ggacgttgat
    12061 gttcatacca attacttctt catatatttt tccaaaaatt tttccatggc cctgtaaaag
    12121 tcaaagcggt tttcttcgtt accaaagccg tgtccttcgt tgtcttttac catatattct
    12181 acttctacgc cacgtttgcg catagcatct accatttggt cgctctcgtt cttatttaca
    12241 cgagggtcgt ttgctccctg agcaataaaa agaggggctt tgattttatc tgcatgaaaa
    12301 accggtgata cttcgcgata gtatgcgcta tctttttcgg gatgccctat ttgctcgtac
    12361 atcatgtcta atattggttt ccaataaggc ggaatggttt ttagcaaagt aaataagttt
    12421 gaaacgccta cgtaatctat gccgcaacaa tacacatcgg gggtaaagca caacccggcc
    12481 aataccgcat atcctccgta gctgcccccg tagatggcaa cacgtttggg gtcggcaatt
    12541 ttttggtcaa tgagccattg aaccccatcg gtaatgtcat tttgcatggt ttttccccat
    12601 tgtttgaagg acgcttccca gaatttgcgg ccatatcctg ttgagccacg gaagttcatc
    12661 tgcaaaacag cataacccct gttggccagg aactgcactt ccgaatcata gccccaacgg
    12721 tcgcgagccc atgggccgcc gtgtggattg atgataactg gcaagttttc agcttttttg
    12781 cctacgggca aagtaaggta cccatgaata gtaaggccat cgcggctttt gtaagttatt
    12841 ggcttcattt cggccaggaa gttttcgttt aaccaggggc tgagttcggc gattttggct
    12901 atgctgtctt tttttagatc ataaaaataa tatgaaccta aagacctgtc gctgtatgta
    12961 cgcacaataa acttatcttc gtctttgttg acgctggtga tagaaatttc gtagcttgtg
    13021 cccaaatgtt tttcaaactt ttggtaaagc tgttttacct cattatcaag aaatacgagc
    13081 ttgcgtttcc aggtagtgta tgaaatagca gaaaggactt tacgtttgcg cgaatagcta
    13141 aggccataca catcatattc ggggtcgctg taaagtacct ttttttcttt tttgcttttt
    13201 gggtcaaaca caacaatggc tttggtgtcg cgtcctttgt tggaggctgc ataaaacatg
    13261 ctgttgtcga aggtgaaaaa cagcgggtca acagattctt taaagtcggt ggtgataatg
    13321 gtttgaaaag agtcgttttc attttcgcgg taaaggatac tggtattggt cccatcggca
    13381 gtgacagcaa ttcggagctt cccgttatgg tcggttaacc aatcctggat gttacctggg
    13441 ttttcggcca gcatttccat tttaccggtg ctcaggtgca gccgatatgg gtcaaaaacc
    13501 ttgttatcgc gtttgttcat ggctataatt acctcatctt ctatatcttc gaggtcatcg
    13561 atgacttgtg tacgtacccc ttcaaaattg gtatagcaca tggggtttga agcgtcgata
    13621 tcaaccccgt agagacggaa gttttcatct cctccattgt ctttcaaata caagatacgc
    13681 ttgttgttgg cccaaaagta tgaacgaata tcgcggtcag tttcagaagt aattctgatg
    13741 gtatctgcac tgcctatttt ttggacaaaa atattcatac gccccttgta aggagctgta
    13801 aatgaaaaat ataaaccgtc gggtgagagt tggaatgctg ctttttcagg gtttttgaaa
    13861 aagtcttcca tgggtatttt cggtacttcc atttgttgag cttgtatagt taaagttggc
    13921 aataatacag caacaatcaa tgaaataaaa atgtttttgt tcatggccga aatatttagt
    13981 ttgaacaaaa gtataaagaa atgaaataca gatttatata aagttataga atctattttt
    14041 gccaataaat gagttttgag aataaaaaaa attcgtttat tttattgcaa aaatatagat
    14101 cgatgtacac tataaaacct ttatgtttat tggtatttgc aattactctt atttcttgtg
    14161 agtacgaacc caaagggtct ttttaccagg aaatagataa ggacattaaa aaccctgagg
    14221 tagaaacaag aagcctgaca tttaataaag ataccattgt tattgaggaa ggtaatcttt
    14281 tgggttatag ctttttcaat gctgacaaaa aaatcagacg gattttgttt aaaattgatg
    14341 ggcaggtttt tgttgacgct tcaacctttt caggtagttt ttctttgaaa ggtgtgtcaa
    14401 aaggaattca cgaattagaa gctgttttta tgctgaattc aggtactggg agtattatgg
    14461 acaaacttgg tggagagctt tatgctacaa aaaaaacgtg ggttttggta gttttgaaag
    14521 ctcattcgga gacaataaac gccacttcag aaaatggctt tttaaaaatc tattgggata
    14581 catgttatga ttttgatttt caatactttg aaattttcaa aggtccaacg ctaattggca
    14641 aaactaaact tcccttttat gttgattcat cttatattgg agagtctgtg acatatacaa
    14701 tcaatgcaaa tcgggctaat tcgagtacaa tatggtgtag caaagatttt aaggaactac
    14761 attatgattt tttgtttatt tcagatcata aacgttgtgt aattaaatgg aacaggccta
    14821 tttgttataa tgctattgaa aaaactataa tttatataaa caacagcaca tttgagacca
    14881 ctgacacaag cattattttg ccagtaaccg aaaatgggca aagattttat tttcgtgaat
    14941 gggaagttag cccaaaatac ggcaacaaca aatatagagt ttttgaacct aatacattat
    15001 atttttattc aagttataaa ttttgtcaaa gtgacattgt taacttatta aaaattgatg
    15061 agtcaaattt tttatattca tctagttcag ataaggattt ttttgaatat tcaataaatg
    15121 acaaggcctt aattgcttct caacattatc tggatgaaat aaaattagtt tctcagagca
    15181 ataaatgtat ttatggaaaa ttaaatggac aagatgccat tcaatcgctg gatgggaatc
    15241 aaaacctttt aatgattgat ggaagtccta atattattgc attgtcagat tattctttta
    15301 tacgatatga tttagacggc tgttatcttg taaaatttgg tgatgagaac aaagtctttt
    15361 tatctcaaaa ctatttagaa aattgtcaaa tgtcttttga tggtaaatat attatgtttt
    15421 cagaaggtgg attattgaaa attaataaaa ttgaaaatga caggctaatt ttaaagggct
    15481 catttgaaaa taatgatgtc aggtttgtca gatttgggtt taaatatccc tcctatttgt
    15541 attattggaa tggtaaaaga tttttggtca aaaattgtta taactttgcc ctaagctctg
    15601 atattctgtt aaatactccc aatactgaaa tactggacat aaatgagcaa aaaaattgca
    15661 ttttggttaa ggttaattcc agtatccata ttatcaatta taccaatggg caaatattgg
    15721 taaacatgga gttaggagat gatgctcgct atgaaaaatt aattcttatg gacaatgcta
    15781 ttgtttctga gggaggttac atctatcatg taaattattg acaaaaaaat tgaggatctt
    15841 tttactcaaa aagaggacaa gagaaaatag attaaactga atttttttaa ggatgaaaaa
    15901 aacattttta ttagttgcca tactgcttat ttttagttca ctgcattcac aaaacctcat
    15961 gtttgggttg aaatcaggcg ttggcagtta cagcatgaaa gatatgaaag atttcaacag
    16021 gtactttgtt tcaaatttgt catttgcaaa caagctaaca gatgattttc ctgcaaatta
    16081 ttactttcag ccagaagtat tgttcaatta tcagcatctt acatttggtt attcttttgc
    16141 tttttattca acgggctcgc ggatagcttc aaatgactat tcggccaatt tcaacatgga
    16201 tgatgtgcta aactgttact ctcatgggtt tatttttgaa tatgtgtttt accgggcttc
    16261 gttgtttgac ttttcggttt attcgaggtt ttcatttgag aatacaaagc taaaaattac
    16321 aaacaacctg atggtggttg ctgaagacac ccttttgcaa actaaataca gctatcggac
    16381 aaagtcaaac tctacagaac ccgggatagc catttcatac gattacaagt ttctgaaaat
    16441 aaagtattat gcgggttaca tgttttcaat aatgaccgat ggttttactt cggatgatga
    16501 aaaaaaacaa aaagtgtata acccctacac ccgcaatcag cttaagcctg attggagtgg
    16561 tttcaggaca ggcatttcgg tatattttat gttttcgttg aaacgaaatt agcggttgtt
    16621 atttgttggc ccggaatatt ttgcctctct caagtctgaa taaacggacg ttttttgggt
    16681 aatgccaaac aacatttcgg caaattgaag gcttaatctg agagctttct aatgtttaat
    16741 tagcgttaac cttcccgtca taaacaggac aaacagattt aggaattgtt tgatgtattt
    16801 tagtaatttg ctgaaataca aatttttagt tgataaagaa ttaacggcaa gccttaaatt
    16861 tttgaaactt ttgcttcaag gctaaagttt gcaatgcttg taaattaaat actttaggca
    16921 ttcttcttat atcaaactca ggttaaatta aggtttaaac gccatttttt tcatttctca
    16981 tcctaaagga ttaatctttt gattgtcttt ttggctaatc gaaaaaacgg tttattttca
    17041 agtttttagc tatgtatttt caatcttaat gaggttgatt agaggctttc atggttttta
    17101 actttacata atagcaaacg tttgttaaaa actacaaaat ataaaagcca tgaatgaaaa
    17161 tatcatccaa aaatttcggg acaaatttat tgatgagtcg gtagggcttt tggaccaaat
    17221 ggaaaaagac ctgcttgaac tcgagaaagc ccctcaggat aaagagctgt tagaatctgc
    17281 attcagggct atgcatacca tcaagggcgt tagtggtatg tatgggtttg aacatatctg
    17341 cgaattcacg catattttag aaaacatata tcagtcgctg cgcgagaaaa aagttttttt
    17401 taataaagaa atatccgatt tgtcttttgc cgccattgac cacatacgcc ggctccttga
    17461 agacgaaaag cttagagatg aagaaaataa aaaaaggcac aaaaaactta ccgatgaaat
    17521 aaacgaactg gctaagcgtt gcaacctttc tcagcctcaa ccggttttta aacaaaacga
    17581 aaaggtgcag gccgaaaaaa ccaaaaattc atggtacatc atcctgaaca ccaatgagca
    17641 aatgtttttc aggggcatta acctgacaag cctctttaaa gagctttcgc agcttggaga
    17701 atatcacata cagcctattc cggtactaac aacacacgaa agcgactcat ggggcattgt
    17761 gctggtatca gactgttcag aaaacgacat acgcgacgta tttatttata tagaagacga
    17821 tatccagatt ttccgaatta ccagcagcga tatttttcac cctgaaaatg aaccaaaagt
    17881 tgagtttgaa aaaacagaac aggaagaaat atcttcaatc atcgacctgg ttgaagaaac
    17941 agaaaagccg cctgtttttt atgctgaaga aaagaaagaa agtaaagaaa ctgaaattat
    18001 caagtctgcc agcttcaaat tacaaaccaa gcgtatagct gttgattcgg ccaagttaga
    18061 ccacctgatg tacttagtca gcgagcttat cacaattaat tcgcaattgg tgcaggctac
    18121 caaaggcagt tattatgcca gccttcagca ttacgtagaa caggtggaca gcctttcaaa
    18181 acaatttcgc aacaatgccc tcgaaatcag gctggtacct ttaagtgatt tagctttgcg
    18241 ttttcagcgg ttgatacgcg atttgtcatc gaatttgggc aaaagtatag cttttgttac
    18301 cgaaggcctt gaaaccgagc tggacaaaag cactatagat atactggctg agcctttgat
    18361 gcatcttata cgcaattgta ttgaccacgg catcgagagc cctgaaaaga gagctgggaa
    18421 agggaagccg gaaaaaggta ctatcaagct ttcggcagtg caatcaggca gttatatcct
    18481 catttctatt tcagatgatg gcaatggcat tgataccgaa aaagtgaaac aaaaagctat
    18541 agaaaaaggt atcattggcc ccaatgacaa cccgtcggac aaagaacttt ttgattttat
    18601 ctttttgcct gggttttcta ctgccaaaag ccttaccgat gtttcgggac gtggtgttgg
    18661 catggatgtg gtgaaaaaga aaatcaccga tttgcgtggc gaaatcatag tagagtcgca
    18721 aaaaaatgtt ggcaccactt ttacacttaa aatccagcaa tctattgcca ttatcgacac
    18781 cttacttttc agggttgaaa attcaagcct cattgtgcct atgtctgata ttgaaatttg
    18841 catacaaatg ccaatagaag agcttgatgc ccgtcgcaac accgaaacca tagagtttaa
    18901 cgaaaggttg ataaaattct acgacctgcg ccaaatgctc agcctgacag gccaatacaa
    18961 agaaaatgta aaattgctca ttatcaaacg cgaagaacaa tttgtagcca ttatggccga
    19021 caaaatcatt ggcgaacacc aggctgtgct gaaaccttta ggcaatacgc taaaagacca
    19081 aaacattttt tcggcagcca gccagcttgg agatggcaac ctggccttct tgctggatac
    19141 ttcggctttg ttcaaaaaca ttaacatttt aatggacagc ttttaacttc aaaaagcctg
    19201 caccacaggt acagcagact attatacatc accatttaaa ccattataac aatgaaaaaa
    19261 ttaatcgcca ttttgttatg catgtaccag gtacatgcta tatgtcagga aaaagctgaa
    19321 aatagcgctg catctttttc ccttaacgcc gatgttgtta gccgatatgt ttggagggga
    19381 ttactatata gccccaatgt caacgtgcag ccttatgctg ctttttcgta caaaggtttg
    19441 tcgttaagca catgggcttc ttacggcatt tcggacagat atgctgaagt ggactttact
    19501 ttgagctatg ccattgataa ccttagcgta tctgtaaacg attattacaa cgagtttgaa
    19561 gacagcctgg ggacaaacag gcatttcaat tttaaagaca acactacccc ccatgcttta
    19621 gaggccactc tttcttataa aatatccgaa acaatgcccc ttactttgtc cttgtcctct
    19681 tttttctatg gcaatgacaa agacagcgaa ggggaaaaca tgtactcatc gtacattgag
    19741 gcttcatatc catttgattt cagcaattat tcattcaacc ttttcatcgg gggtaccctg
    19801 gacaaagggt tatatgccga caaagccgct attgtcaatg ttggtttttc tgctaccagg
    19861 cagataaaag tatctgatag ttttcaactg ccttttagtg catcgttaat ttctaaccct
    19921 tatgctgaag atatttttat ggtactcaaa ttgagtttct gacctgaaaa tgaaccttgc
    19981 aaacaagaca ataaaatgca ggtattccat ctgcttacat tatatggcaa atattgatgg
    20041 attaaataat tgaatttcaa taaattaaaa tttataaaga tgaaaacaca aaaatttgta
    20101 tcgctaaagc aaaagttggg tttgattgtt ggaacaggga taaccctgac ggcgcttgta
    20161 ttaatctctt attcatcaat aaaaacccgg acagattcga taaattcggc aaaaagcaat
    20221 gccttggcgt tggctcagga ttttgcggtg aatatctctt tagaacttga aactgccata
    20281 tcttcatcaa ggtcaatggc caatgccctc tcggctgttg gcgacccaaa atcgcccatc
    20341 aggctatcgc gtgaagaggc tatgaaaatg gccgagaaag ttttgttttc gaaccccaac
    20401 tttttgggct ttactttggc ctttgaaccc aatgcctttg acgggcagga tgcaaaattc
    20461 cgtaacgctg aagcccatga caaaactggc cgttttctgg tatatcaaac caaaaaagtt
    20521 gataatacag ttgcccgtga ggtgcttatt gattatgacg accccgaaaa agccccttgg
    20581 tattgggtac ccaaaaaaac tatgaacgaa tttttgacag aacccgttat ttaccctgtt
    20641 cagggagtgg acgtgctcat ggtatctttc atgaccccgg ttatcaacaa tggccgcttt
    20701 ttggggaaca ccggcataga ttaccctata gatttcatgc agcataaagt taaaaaagcc
    20761 ggttattatg actcgttggc tatcatcagc attatatcta acgagggtac atttgtggcg
    20821 cattccaaaa cccccgaact aataaaccat tcgctaaata ccatttattc agatgccccg
    20881 ggtcaaatac taaaaataaa aaagggcgaa aaacaaatat ttgtgcaaaa cgataccctt
    20941 tcgatacatg tgcccttgaa tattggacaa acccaccggc catggcaggt acgcatggaa
    21001 gtgccaatga agataattac agcagaagct aatgaacaaa tgtggtttca gctttctatg
    21061 ggagtaatcc ttattattgt cagcatagta gtaataaggc tttttgtaaa acgtatcatt
    21121 aagcctgttg gtattatgtc tgaaaaagct aaagctgcag cagaaggcaa cctggtttac
    21181 gaaaccatag gcgacaaaac caatgatgag ataggacttt tgtccgatgc actcgatacc
    21241 atgatagaaa aaataaaaga tgtagtgtca aacgtgataa taagctctga aaactttgtc
    21301 gaaagcagta agcaacttag tgcttcagcc cagcaaatat cgagcggggc aaatgaacaa
    21361 gccgctgcca gcgaagaaat atcttcatct attgaagaaa tggcctcatc ggtcaaccaa
    21421 aatgccgaaa atgcccaaca aaccgaaagg ttggcctcga gtgccgccca aaacatcaaa
    21481 acagccaacg atgctgttat tgaaaccatt acagcaatga aaactattat ccaaaagata
    21541 tctatcatca aagaaatagc cgaaaaaaca gacttattgg cggttaacgc agccatcgag
    21601 tcggccaggg ctggcgaata tggcaaaggg tttgcggttg tcgccagtga agtacgaaaa
    21661 ttggcagaac atagccaaaa tgcagcaaaa gagattgacg atatttcggt ctcgagtctt
    21721 caaattgccg aaaaatccgg acaaatgctc aatgccgtga tccccgaaat ccaaaacacc
    21781 gcaaagcttg tacaggaaat taccgctacc agtattgaac aaaatggggg tgtctcgcaa
    21841 ataagcaatg ccatccaaca actgtcaaca gtggtacaac aaaattcagc cctttctgaa
    21901 gaactggcag ccagctctga agagcttacc gggcaggcta cgctattgtt agagtcaatt
    21961 tcatatttca aaatatctaa aaacgatatt agcaatgaaa cagatgaaga aattgaaaac
    22021 cagatcaaaa aactctcagc ccttctcgat tcgcgaaaaa aaggcccaaa taaaaaagaa
    22081 acccaaataa aacaaaaagt gtcagaaccc aaaaaagacc ctttaaaagg gtttaaaatt
    22141 cacttagatg acaaagaaaa tgataaagac aaagaatatg aatcaatgta aaaattaaat
    22201 taataccatg agcaacacat acttgtcatt tgttgttggc gaagaacaat ttgccgtcaa
    22261 cgtaaccaaa gtactggaag tacttcaaaa gcaaaccatt accaaagtgc ccaatgcccc
    22321 ggactttatc aaaggcatta tcaacttcag gggcgaagta gtgcctgttt tcgaaatgcg
    22381 ggtaaaattt aaccttgccg agcgtgacga aacaaaaggc tatgtaatca tcatccttga
    22441 tttacaatca ggagaagaaa ccatacgtat tggggccatt gcagataagg taagagatgt
    22501 tatcaccctt aatgatagcg acataaaacc tgtacctgcc atgaacaaag attttaatac
    22561 tgaattttta cagggcattg tcaagctgaa cgacaaattc atcctgctgc tcaatgtaga
    22621 taaagtattt acaagcaatc agttgcagca actccatgat agctctgaag ttttaaaaaa
    22681 tgcataataa ataaattata aaaaaaaatg aaaaccatga aaacattgaa caattttagc
    22741 attgggagcc ggttgacctt tgtatttggt ttaattattg tacttagcat agtaaacctt
    22801 tttgtgaacc tgcgaggcct cgacaaatcc agcaaagcca ttgatataat gcatgtttcg
    22861 caaaccagta tctcttattt gctcgaagcc gaccgcgatg cctaccaatc gagtattggt
    22921 atcagtcaat gtttagacaa agagccaaat gataatgctg aattagaagc ttatattacg
    22981 gacattttag ataaccgcgg acagataaaa acccgttata ccaaatttgc cgaagaattt
    23041 aatgtcaagc aaaatcaggc ttatgctgcc attgattcta acttttggtt gaaatataac
    23101 gatttgtgca cttattctga tgaaatagta aatcatttaa aagcccgcaa atacaaacaa
    23161 gctgctgcta tctatttcac agactatcag gatgctttta agccaatgcg tgaagctatc
    23221 aaccagttta ccgaaattca ccaaaacgac tctcaaaagt catacgaaac caacaaagaa
    23281 gtgagcagga atatccgcat caattctatt tccatattta tagccatagt cattatcttc
    23341 ctcatcagtg atatcctttt aacacaaagt atagccaaac cattgtcaaa tgcagtgaat
    23401 attaccacca aaatttcggg cggcgacctc accgaagagt ttgaggtggt tggaaaagac
    23461 gaaaccagca aagtactggg cgctattaaa gccatgtcag gcaaaattag tgaaacggta
    23521 tcttctatta agatttcggc cgaaaacttc ttcgaaagca gcaagcaaat aagcgcatca
    23581 tcgcagcaga ttgcttcagg ggccaatcaa caagcttcgg ccagcgaaga gatatcttcg
    23641 tcgattgaag aaatggctgc cagcatcaac caaaacactg ataatgctaa gcaaaccgaa
    23701 aaacttgctt tgcaagccgt tgaaaaccta aaaattgcca atgactcggt agctcatacc
    23761 ctgaaagata tgaagatgat tatccaaaaa atatctgtga ttaaagaaat agccgaaaaa
    23821 accgatttgc ttgctgtaaa tgctgctatt gaagctgccc gagctggcga aacaggcaaa
    23881 ggctttgcag tagttgccac agaagtacgt aagcttgccg aacacagcca acaagctgcc
    23941 aaagaaatag atgacctttc tgcatcaagc gtccttattg ccgacaaatc tggacaactc
    24001 ttagcatcag ttacccctga gatacaaaat atagcaaagc taatccagga aatatcggct
    24061 accagtatcg aacaaaacac cggcgctgcc caaatcagcg cttccgttca gcagctttcg
    24121 caagtagttc agcaaaatgc cgcgttggcc gaagaattgg ctgccagctc cgaagaactt
    24181 accgggcaag cctcatcgtt gctcgacaac gtttcgtatt tcaaaaccag tcgcgaagaa
    24241 attgacaaat acaccgacga agaattcaat atgctgatga aacgtatcaa cgaaatactg
    24301 gctgcaaaaa acagaaatgg gggcgctgaa cctgctccat tccaaaaaaa cctcaaagcc
    24361 gaaaaaaatg ttgttgcttc ggtaaaacct gcagctaaac ctgccccaaa agatggcctt
    24421 gacataaaat tagaagaaaa agaccctgac tacgacaagt tctaagagtg tataacatcc
    24481 tgtaactatg gcaaagctgc aaaactttgt catagtccct tacaggatat tgttatcttt
    24541 gccttttgaa aaaatgaaac gttaagccag actttcaaaa agaaatatga tgaaacaccc
    24601 atgattattc aatcgcaaac aaatatttat cggcaggtgt tccatccgtt caccttaaag
    24661 catataatga tggcttaaat tgctaatttt taacaagtta gaattttata cggatgaaaa
    24721 aaaatattat atcattagca agtttagctg ttatgctttc actgggacaa gctgaagccc
    24781 aggagacaca agctaaaagt gggaccgaca cattggctcc aatggtcaaa gaggtgcaag
    24841 ctgatttgaa aaagctgaaa aaaattaaaa tcagcggata catacaagga caataccaaa
    24901 aagccgaatc aaaagggatt gaaacattta gtggcgacga tttcccttca gacattgacc
    24961 aaaggtttgt tacccgccgt ggaaggctca aaactacata tacaggaaaa cttagtgaaa
    25021 tggcaatcca attggacgtt actgttgaaa aaggcgttgc cctcaaagaa gcctattata
    25081 aggttacaga accagttttg aaaacagtat ccctgcagtt tgggcttttc atgaggcctt
    25141 ttagtcatga attaaattat tccaatacca tcatggaata tcccgaacgt accagagtag
    25201 tacaaaatgt attgtcggga gaaacagacc ttggggcttg cctgatattc cagctaccaa
    25261 aagaaaaccc gctaagtgct ttgcgtcttg actttggcct ttttaatgga acaagcaaca
    25321 aaggggctga ttatgacaat gagaaagact ttatcggacg attgtcatat aacaacactt
    25381 ttgctgacaa atatattact ttaggtgccg gtatttctta ttttaacgga ggtacatgga
    25441 attttgcaac taactattac gagtggaaag atactgcatt tacaagtatt acagcggaca
    25501 gccttaaggt actcaaaaaa aattacatgg gagcagatat ccagcttacc ctggataaca
    25561 gttttggaca aacccaaata cgtggcgaaa tgatgtgggg gacacagcca acggccaatg
    25621 ataaaacaaa aacatttctt gaagcataca aaaaacctgc ttatatgcgc gatttttcgg
    25681 gtgggtacgt ttgcctggtg caaactattg caaaaacaaa acatcagttt gtggtgaaat
    25741 atgattggta cgatcccaat acaaaagtaa ataaagatga ggttggtgct aaaacgccat
    25801 caggagcaac aaatttgact acaaaagcag atattgctta tactacctta ggcctgggct
    25861 ggatatacaa ctgggacgaa aatgtaaaga ttactgccta ctacgacatg gtatctaacg
    25921 aaaaaaccct cttgaagggt tataccgaag atttgaaaga caatgtattt actttgaggg
    25981 tgcagtacaa gttctgatag cttttttcaa taatacaaag aaagtgccag tagcctttga
    26041 ggtatcaaac tgatatggtt tatctgcaac aggaagctca aaaaccaggt tttcagcaca
    26101 aataaaggct atgcagccta ttggttaaaa attcaattca ttttttaaag caggatttcc
    26161 gaaagagaat cctgcttttt tttcagcatt taattgtagc tgttgaggta tttatccttt
    26221 tcattgagct tgaaatagct gatagtttcg gtaagttggc tggcaagctg gttaagtttt
    26281 tcagctccta cagccatttc ttcgcttgat gaggcatttt gctgggtatg gttattaagc
    26341 tgctggatgg cgttgtttat ctgcacagcg ccagtgtttt gttcaacgct ggcagtggta
    26401 atctcttgca caagactggc tgttttgaca atatctgcaa tgatagcttc aagcagttga
    26461 gcagagtttt cggtaacgtt gacactttgg tttgccaggg agacaatttc gtcagcagct
    26521 attttactgc gttcggcaag tttacgtact tcggcggcaa ccacagcaaa acctttgccg
    26581 ttttcgccgg cacgggcagc ttcaacagcg gcattcagcg ccaaaatatt ggtctgaaat
    26641 gcaatatcat tgatgatgct gattttactt gcaataacct taatagagct aaggctttcc
    26701 ctggatgaat cacctacttt ctgaatacct tgtgatatgc tgtgggctat tttttcggtc
    26761 tgctgggcat tgtcagcagt ttgctggatg ttggccacca tttcttccat cgacgaagat
    26821 acctcttcaa ccgaagcagc ctagtttgaa gcgcttaccg atagctgttg cgagcctaat
    26881 tgtaattgtc tgctcgaatt attaaggttt tttacgttat tatttatctc agttacaacc
    26941 ttagtgaggt tttgtttaat tttttcactg gcaatgataa gtttaccaag gtccctatta
    27001 gggttcacgt ccttagtgtc aatctctaca tcgaggttgc cattggagat ttcttccagt
    27061 ttttctaccg atttggacaa aggcttcact acggcaatag aaccatagta aaaacaaatg
    27121 acgctgacaa tgatattgct cagggtgatg gcaatgacaa caggcttatt gtcctcaaaa
    27181 aatttgtact tgaggcccac cataaacata ataaaaagca ggttgaacaa ccatacaatg
    27241 ccaacacgaa caaaaacaga gtttttaaaa aaacggttga gcaacaatac tgcagcaagc
    27301 ccggctgctg caatactaat caaattactg ataaaaaaat tcataataaa aagcttaaaa
    27361 ggttagttta ttttataggt tcagaattaa aggtatttat ttgcaaaata atgagtttta
    27421 tcaattttat ccttgatttt tcagaaattt ttagcactga aaaagtgtat ttgtttcaat
    27481 acaattaaat gtcatcttac catagtttgg aacgttttta ttaagaaaac tttatcaggg
    27541 aaatgtacct tgcctgaaaa tgacagtttt ttgaatgcat tttaacacgt cttttgcact
    27601 cttctggttt agttttgata agtttgaatt taaaaaaact aaacacatga gaatattgga
    27661 aaataaacaa ctggcaaatg gtttagcgag tttactaata gtggctttta gcttttcgaa
    27721 cacgacaaaa gcccaaccat ctggcgggcc ttatggccct attaagcaaa cttatgaaat
    27781 acctaaaact acaggagtta tatactatgt agcccctgat gggaaaaaag aagaacaggg
    27841 tttgtcgttg gacaagccta ccaccatcga atctgctata caaagggcta aaaccggcga
    27901 tgtgattatt gtccgtgggg gtatataccg cacgggttca ctcttgctca accaggggat
    27961 aacaatacag ccttatgctg atgaacatcc tgtttttaaa ggaacttatg ttgcaaaaga
    28021 atggaaagac cttaaaaatg gcacctggtc tgtcaaatgg gagcacttgt tcccctcaaa
    28081 acccgataca tggtggaacc gtgcaaaata catcagggaa accccattgc accgcttcaa
    28141 caatgacatg gtgtttgtca acggaaaatt tttgcaatct gttgggcaag aaaacgaggt
    28201 tgataaaaat actttctatg ttgattatga ggccggaatg gtgtatattg gcattgaccc
    28261 gaccaacaaa ttggtcgaaa ttactgcatt cgacagggct atacacaggg tgaccaccga
    28321 atgtaatggg aaaacatcgg acaagaaagg gtttaccctc aaaggcattg actttatgca
    28381 atacgcctat catggtatcg aggtggatgg caaagaccct gaggctgttt caaatgaagc
    28441 cgaccatgga aaagatgttg taggcaccct aattgaaaac tgcagtatta ctttctgtgg
    28501 ccgtgttaat gtttttctca aaggcgataa aattaccctt cgtcattgca aaataagcga
    28561 aagcagtacc gaagggcttt atattgttgc ctcaaatgac gtgttgctcg aaaaaaatat
    28621 tttttcgcgc aacaatatcg aaaatatcac cggctattat ccggctgccg tgaagatatt
    28681 caaccaaagt taccgcgtaa cctgccggga caaccttgtg accgatttgg ccaactctaa
    28741 cggtatttgg tatgatgttg gaaatgtcga tggctgcttt atcaacaatt gggtagaaaa
    28801 tgtaggcgat ggaagcatca aatttaacaa aaacaaaacc tggccaagtc aaaacggctt
    28861 tttctttgag atttcaaaag gggtaacttg tgccggcaat gtatttttca actgcgacca
    28921 gggtattttt atcctcaaca gctgcggggc aaaggtgtac aacaacacat ttgtaaacag
    28981 cacagctact ttcgggcgta acgaacgaaa cccggccaat gacggcacgt ttggatggca
    29041 ttcgggcact ggccctgatg tagataaacg caccggtcat gttttttcaa acaacctcat
    29101 gtttagcgat gcccgtcacg aaaggcctat gatgtttatt tggcagcccg atacttttta
    29161 caaaaggaat tataaacccc aattagaaaa gctcgaaaac aatgtaatgg tcaaaatccc
    29221 cagcttagat acaattgtcg caatatattg gacacaggct gttgacaaga gttgtcttat
    29281 ggcttttcaa aactttgaca gcctgaaaaa aacatacccg gaggtaaagg ataataaccg
    29341 tttttacact gacataaatg tttttaaaag tgttgaactc aagcagttaa cggtgctgaa
    29401 gaattttcct ggcaatatag atgcaggcct ggtgcctgcc aacattagcg cgttgatgaa
    29461 cctccctaag aaagattaca aatttgttgg ggcgtatcct ccaataaagt aatgaactta
    29521 ttattttagg gcatttatga ataataaaat gcatataaca aatcatggcc tttgaccgtg
    29581 gtttgttatt taaactaatc atcataaaaa atgaacagaa atgtattttt agcttgtata
    29641 atattttgta tttggcggtc tgtgacagct caacatcttt taagccgtca acgcatcctt
    29701 atcaatgaag gttggcgctt ttataaatac gattcggcct cgcaagctga caatctcata
    29761 tacgatgtac gccctgaaat aaaggacaat accgaaagca gggtagcaga tgctaaacct
    29821 actgaagctg ttgaaacaaa atcgtcacaa aatgtactaa aagcctgggt tctgccttcg
    29881 gccaacgcgt ttatcaaaga cccgcaaaaa cgtcatctac gtccagatgg aaaccccggt
    29941 agcgactttc gttttgttca aaataatttt gatgataata actgggagca tattgacctg
    30001 cctcatgact gggccatcaa aggccctttt cagaaaggct gggactcaga ggttggagga
    30061 ggtatgggcc gtttgcctgt caatggtgta gcatggtatc gaaaaaaact tgatattaca
    30121 gcttctgaca aaggcaaaac tattattctt gaagtagaag gggctatgtc gtatgccata
    30181 gtgtggctaa acggcagttt agttggcgga tggccttatg ggtacaactc atggcagctt
    30241 gatcttaccc cttacgttgt gccgggtggt gaaaaccagt tggctattcg tattgataac
    30301 ccaaactatt cagctcgctg gtatccggga gcggggattt accgaaatgt atggctgata
    30361 aagaccaacc cggttcatgt gggacaatgg gggacctata ttaccaccaa aaatgtttca
    30421 aaaacttcgg caaccatcaa tctggcggtt attattgaca acaacacaac taaagaagct
    30481 gaagttgagg ctactacaca aatatttgcc ttagattccc ttcttcataa gactggtaat
    30541 gcggtggctg ttttttctcc aataaaaaca aagattagac caaaagaaag ctcaaaagtc
    30601 gaagctgtaa ttgagcttaa cgctccaagg ctttgggggc cgcttccaac acagcaaccc
    30661 aatttgtatg tagccttaac caccttattt caaaacggaa aaacaataga ccagtacgaa
    30721 acccgctttg gcatacgttc tgtagagttt gaccctgaca aaggcatcaa ggtaaacggt
    30781 gaacgcatcc aaattaaagg tgtaaaccaa catcacgact tgggggctct tggcgctgct
    30841 ttcaacaggc gtgccgcaca acgtcagttg caaatactgc aagagatggg ctgcaatgcc
    30901 atccgtatgg cccataaccc tccagccccc gaacttctcg aactgacaga ccagatgggc
    30961 ttacttgtta ttgacgaaat ttttgattcg tgggaacgca aaaaaacacc gtatgatttt
    31021 caccttattt ttcccgattg gtctgagcct gatgcccgtg cctttgtccg tcgggataga
    31081 aactgtcctt cagtaattat atggagcttt ggcaatgagg ttggcgagca atatactgct
    31141 gaagaaggtg ctgccgttgg aaaacgactt tataacatca ttaaagaaga agaccccacg
    31201 cgtcctacca tcacggccat gaactacgcc aagcctgaca tggctttgcc tgctgtagcc
    31261 gatatgattg gcctcaatta ccaaggtgaa ggcatacgtc aggctcctga gtttgaaggc
    31321 accgaacgta ttcgtacagc tccgcaatat gacgcttttc ataccaggta cccgggaaaa
    31381 gttgtcctca gcaccgaaac tgcctctgcg gccagcagcc gtggaatata cctgttcccg
    31441 gtaaccaaag aaaccagcgc ccctatacgt gacgggcgtg ggggcgattc aaaaatatgc
    31501 caggtaagcg cctacgaact tcatgccgta gattttggtt cttcagctga caaagtcttt
    31561 ggtaccattg aaagacgctc ttttgtggca ggcgaatttg tatggaatgg ttgggactac
    31621 ataggcgaac ccacccctta ctacgaagcc cgcagttcat ataccggaat aattgattta
    31681 gccgggttta aaaaagaccg tttcttcctt taccaggcgc actggcgccc cgattatcct
    31741 atggctcaca tattgccaca ctggaactgg ccagcaaggg taggggaggt tactcctgtt
    31801 catatattca cttcaggtga tgaagccgaa cttttcttaa acggtaaatc actgggcaaa
    31861 caaaagaaag cacaatacga ataccgcttg cgatgggaca gtgttattta cgaacctggc
    31921 gaactaaagg ccattgctta taaaaatggc atcagatggg ctgaagatat ttttagcact
    31981 accgaaacag cttcaaaaat agagcttact gctgaccgtg ctacaattct tgccgatggc
    32041 aaagatttat cttttgttac tgtgcgtgtg acagataaaa atgggcttac tgtacctgat
    32101 gcctccaatc tggtaaagtt ttttgtagaa ggccccggcg agattgtagc tacagacaat
    32161 ggcaatgcta tggacttagt ggcatttccc tcaacagcac gcaacgcttt tagtggcttg
    32221 tgtttggtta ttatacgagg atataaaggc cagacgggaa ccataaaact tatcgctcaa
    32281 gcagaaggtt taatatctgc ctctttagaa atcaatatca aataacctga ttcctgcctc
    32341 taaaatcaat aaagaacgtt caaaaagaag aggctgtgtt ctttgttgta aaacttagaa
    32401 cacagcctct taatccaata atcccttatt taaataacaa agggacaaac tccgaaaggt
    32461 aaacccgcca attggtccaa acatggccgc cttcggtttc tacatacgta tatttcaaac
    32521 caatggcatc aagctttgcc cgatagtcaa ccatatcctt ataaagaaaa tctgtttttc
    32581 ccataccaat ccaataaagc ttacaaccgt tttctttttg ttttttcagc gtcacgtcaa
    32641 tatcactgta aaaagggatc ggctcttttg caagtgggtt aacagcagcc gaaaacagcc
    32701 caacataatc aaatgtatta ggataagtgc gggatacgtg caacgaatga aatccgccca
    32761 tcgacaaacc gcatattgca cggctgcctt tttcggcttt tacccggtaa ctgctttcga
    32821 caaacttgat aatatcttta aaagatgttt caaacttacc atccatgctg tttggtaaaa
    32881 agaaatttgg tttatacatg ccaaggcttg attcgccagg agcagcttcc tgaacaacat
    32941 tgccattagg cattaccaca atcataggtt tggctttgcc ttgcgcaata aggttgtcga
    33001 gtatttgcga agccctgccc aaagctatcc aagcttcttc gtcgccaccc atgccatgga
    33061 gcagataaag aacaggataa ctctgcttgc tcgtttcgta tcccggagga gtgtaaatag
    33121 taatgcggcg tgtcaaccca aggcttggcg aatcgtacca acgccgcgta acagaaccat
    33181 gcggtacttt gtttactttg tacaaatcgc ctttgccatt accaacaata aaaacattgg
    33241 ccactgttgc cacatcgcga atttgataaa cattattagg gtccgaacac tttaacccat
    33301 cgacaataaa gtaataacta tacaaatcag aggtcaaagc cgcggtagtg taagtccata
    33361 cgccgctatc gcctttggtc atgagggtcg agccaggttg ccagttttca gcaggcatcc
    33421 attcgcccga aattttaact tctttggcat taggcgcgac aaaacgaaag gtaaccgtat
    33481 tgtcgttttt tatttcgggc gaaatgatat tcgctcctcc ccataatgct tgttgagctt
    33541 gagatatgtg cattgtcaat aatgcagcag caataaaaat gagttttttc ataatattaa
    33601 gagattagtt tgtttttaca aaaaattctt atccaaaaat tcctagaact tgtctttgta
    33661 ttggtcgctg acaggtatgt aagtactgcc aaagatgatg cgtgaacgct caatagtttg
    33721 aatacgctca aggttgacaa taaacgaacg gtgtactctc ataaatttcg attccgggag
    33781 cttggcctca accgctttca tagaactgat agacaatatt ggctttgggt cgttttctac
    33841 tacgactttt acatagtctt tcaagccttc gatatacaaa atatcgtcaa aattgatacg
    33901 ccggattttg taatctgatt tgataaacaa aaactcggta tttgcctcta ttttattttt
    33961 attcgcagat ttttgttcca gctcaataag cttgcgggct ttgtgcgatg caaccagaaa
    34021 ttcttcataa gcaaaaggct ttagcaggta gtcaatggct tcgagcctga aaccttccaa
    34081 agcgtacttt tcgtaagccg tagtaaaaat aattttggcc ttggcctgca atgttcgggc
    34141 aaactcggtc cccatcaaat cgggcatttg aatgtcaaga tatataagct gcacagggtt
    34201 gtcatcgaga aattcgatag cagacagcgg gttgtcaaaa caacctttta gctccaaaaa
    34261 aggcgttttt cggatatacc cctcaacaag ctgtaaagcc aaaggttcgt cgtctatggc
    34321 aatgaccgaa agcttcatat attaataatt tccagttcta cttcaaaagc tttgtctgat
    34381 tggtttatat ataacctatg tttgccgggg aacaacaaat tcaggcgttt ccgtatattt
    34441 tcaagcccaa tgcccgaacc tgcatcattt tctgtttttg acacgaggct gttcctgcaa
    34501 acaaaattga ttttttcatg tcctacattg agctctatgt caataaacga aggttcgcga
    34561 tagctaacac catgcttaaa ggcattttcg acaaacgaga taaacataag cggcggaaac
    34621 tttgagtctt tgaactttgc aggaagcgcc acgttcagcg aaaccttgtc gtttagccgc
    34681 aacttcatca gctcaatata gtttttcaaa aaatcaattt cgctggaaag cgtcgtgtct
    34741 tccttttcag attcatacaa caggtaacgc atcattttcg agagtttcag gatggcatcc
    34801 tgtgcatctt tagtgtttat ctcggtaagc gaataaatat tgttcagcgt attgaaaaaa
    34861 aagtgagggc tgacctggtt tttgagcagg gccaattcag agctgagctt ctctttttca
    34921 agctctttgc gctttttctc gttttctata taacggccat tcatccttag gccaatactg
    34981 aagccagaaa ccagcactgc ggtaaacaaa tagttgtata tatcaaactt ccacgggtgt
    35041 tttggaggct tcatttcccg tgcggccctg tcaaagactt tattcagctg ctgttgctca
    35101 ttgacaaata caaggcggtt gaccgattcg ttgacaaagt ataaacttgc cactaaagca
    35161 accaaaatca aataaaactg gagttttttc cctttaaaca acagccacgg gatgagcaaa
    35221 aagaaattga tgtaaaatat caggatgtaa atgatggtcc gaaagtaaat acgctggatg
    35281 aaaaacatat ccatgccccc gttttcgtga ataaagtaca aaggcaggag gataaacgtt
    35341 gcccatgcca ggatgtgcag aaacacagcg atttttttgt cagaaaacca attcatctcg
    35401 ggtttgtttt acttatcaaa tctacaacat tttattcgta gcgcaaagca tcaatagggt
    35461 caagatttgc tgccttacgt gcagggtacc aaccaaaaaa tacaccaata acggtacaaa
    35521 caagaaatgc taacagcaca gcttgtggca tgactacaat tggccagctc attattgtac
    35581 caatagcggt tgaagccatc atgccaagta ctatgcctat tactcctccg gtaacgctga
    35641 gcaggataga ttcggtcaaa aattgcataa gaatatcaat gcccctgcca ccaatagaca
    35701 tacgcaagcc aatttcgcgg gtacgttcgg taaccgaaac atacatgata ttcataatgc
    35761 ctatgcctcc aacaagtagc gatatgccgg ctatggcccc caaaaggatg gtaagtatgt
    35821 cactgataga ggtaaacatt ttgatgagtt cttcctgtga gcgcacttca aaatcgtcgt
    35881 cttcgccgtc ttttatttta tgtttttgac gaagtatggt ttctatttcg gttttggcca
    35941 aagctgattt gtcttcgctc accgctgaaa tgtagatact ttgcacgtgg gttatggcca
    36001 ggaggcgttt ttgtacagtt gtgtatgggg caatgagcaa atcgtcctgg tcttgcccaa
    36061 aattattttg gcctttttcg gccaaaatac caataatttt gaacgggata ctattgaaac
    36121 gaattatcat gcctgtcggg tcaaaattct cgccaaaaac gtttttaatt acagttttgc
    36181 cgataaggca cactttagcc gaagagttga tttcggcctg ggtaaatata cgccccgatt
    36241 ttattgatac tttacgaata tcgagatatt cggggttaac cccgtaaata gttgtagggg
    36301 tatttttgtt gccataaata acctggccac tggtgcgtac ttcaggtgat acacgcgaaa
    36361 tggcagtgct attggcgcgt attgcatcaa tatcgtccag cgtcaggctt ttgctgctgg
    36421 cattgcccat ttgtacgccg cctttgaact gaccgcccgg cataacaaac aacaagttgg
    36481 agcccatgct tgacacttgg tcctggatgc ttttttttga gccttgtccg atagcaagca
    36541 tggttattac cgaggcaaca cctatgataa tgcccagcat ggtaagaaat gcacgcatct
    36601 tattgcgccg caatgcgttg atggctattt ttatgagatt tgtgtaattc ataatgttgt
    36661 tttttaattc aaaaagtttt caataaactt ctgtggcttc ttaaataatt gaagctatta
    36721 ctcctcctct acaaaagcag gaagtatggt tggtttattc atacccattt gttaccggca
    36781 tctcagccag caattgtttt gcatcaaaac ggtcggcaat tttttcttgt ttgatgatat
    36841 gtccgtcttt aaaaatcaat gttgatttgg taaatcgggc aatgtctggc tcatgggtga
    36901 caaatactat ggtttttccg ttattattca gttcctggaa caaggccata atctcataag
    36961 acgttcgggt atcaaggtta ccggtagctt catcggccag gattactacc gggtcgttga
    37021 ccagtgagcg ggcaatggcc acacgttgct gttggccgcc tgacatttgg tttggcatgt
    37081 ggtgcatacg gtctttaagc cccactgatt cgagtgcata catggccctt tctttgcgct
    37141 cttttgcctt gactgtagag ttataaagca gcgggagctc aacattttcg agtgctgtag
    37201 tacgcgggag cagattgtaa gcttgaaaaa caaagcccag cttgcggttt cgcaaattgg
    37261 caagttcatt tcggctcatc tgggccattg ggtgcccatc aagaaaataa tcgccactgg
    37321 taggcttatc taagcaccct aagatattga gcatggtgga cttccccgag ccgctggccc
    37381 ccataatggc cacaaagtcc ccttcttcta tttcgagcga taccccgcgc aatgcatgta
    37441 cggtaacttc gcctacatga aagtctttgc gcaaattacg gacttctata atctttttca
    37501 ttttcaattt actttactat tttaaacgca atggcacgac gatacaaagc aatatccttc
    37561 agcgtcattg cgccttagca ttctaaaaat tggtttactg tggccttgga gggttactac
    37621 ccgatttttg tgaagggggc tttggcataa acgggctgct cgatgtccct ttggttacaa
    37681 ctgtagaacc tgaatttatt gctgtaatta cttcgtcgcc ttcttttagg ccgcttatta
    37741 cctgtgcatt catatcgtca tcaataccaa tcttcactgg tacaggaaac accatttctc
    37801 cttttttgag ccaaactatt tttgcagtag aatctggctt ttcatgttta gtactgccag
    37861 tttggccatc aggcatgcct tctggctttg gggcttcctt atcgggacct tttttcttca
    37921 ttacactttt tttatactct tccattactg ccatatctgg tgtaaaatgt aatgccttgc
    37981 tcgaaacagt aagtacatct atggcttctt caacaaatac ttttatactt gctgtcatac
    38041 ctggcataag cttataatca ggatttgggg ctttaacaat aacagtatag gtaactacgt
    38101 ttgattttgt aaccggctgc aaacgcactt gtgtaacctt gccctcaaac ttcaaatccg
    38161 aaaaagcatc tactgtaaat gatacccgtt gcccttcttt tatttttcct atatcagcct
    38221 cgtcaataga agcttctacc tgcatctggg taaggtcgtt ggcaatgcta aacaaagtag
    38281 gggtattaaa gctggcagca acagtctgtc cttcatctac agccctttct aatacaaccc
    38341 catcaatggg cgaagtaata gaagcatagt tgaggttgat tttggcacgg ttatattcca
    38401 attttgctgt ttttaaagct gcttcggccc tggtataatt gtaaagggcc tgctcgtaat
    38461 cattttgtga tactaagctt ttctcgttca atgcttttat acgattgaag ttggccgtct
    38521 gcaatgtcat ctcggcctgg gcattttcca tatttgcttc agcgttttca agactggttt
    38581 gcaatgtccg ggtatcaatc tgggctaaca actgtcctgt ctttacatgc gaattgtagt
    38641 ccacgtaaat tttgtcaatg acccccgaaa cctgggtacc tacgctaact gtttttatag
    38701 cctgcactgt acctgttgct gtaacggtat tacttactgt gccctttttt accactgtgg
    38761 tttcaaaaga tatattcgca gcttgttttt tacagccttt tgcaaaggct atgagtatca
    38821 acactgcagc aattgctgaa acccatatta tcgttttctt cttcatataa ttgattttta
    38881 tcatttttta tagtgtcaaa ggttttccca aatagaaatc aaggattttt ttgctaaaaa
    38941 ccaggttgta cttcgattgc aacaagtcgc tttcggctgt aatgaggttt gttttttgaa
    39001 tcagaaaatc tactgaactt aacgaaccca aactgaactt ttcttgtgct acattgtacg
    39061 cctcctgcgc tgagttgaac ttttcaaggc tggcctggta cttgacataa gctgacttca
    39121 agtttacaca agcctgttct attgactttc gcaattggtt ttttacgtcc gtctcattca
    39181 aagaggcttt ggtagtacca attttggcta ttgctacact ggagtgtacc tgcctgtttt
    39241 gaaaaatagg aatcgacagc gtcaaaccca cagatgggtt tagcttattc tttacctggt
    39301 aatcataatt catgcctact ccggaaatat aaccagtgct taaccctgca tttaatgtta
    39361 atgtaggaga atatcctgat ttagctattg atacatttac ctctgctatt tgcttattaa
    39421 gctcagccga tttaatctgc ggctttatct caagcgcttg tgagtaaacc gagtcggcat
    39481 tgtattcagc gttaaacgcc tcagagattt ccatgtcagg atgctcgata gcaaacgaac
    39541 tacttaccgg gagctccatc aactgcatta aagaaacacg attcactgcc aactggtttt
    39601 gtgcattggc cagcgtaagc ttttcggtgg ctaactgtga attcacctgc aaataatcgg
    39661 cccgtgaaat agaacccagt tttaaccgct cttctgaaag ggtaagttgg tgagaagttg
    39721 actctatttg tttttcactg ttcttcacct gctcttccga atacagcact tgcaaatatg
    39781 catcaagtac atttaaactt attgcttctt tcaatgtctc aacatcgtaa ttaccggcct
    39841 gataatttag ttcggcctgc ttaatatcat tagtggtttt aaaaccatta aagagcctga
    39901 ggcttgaatt gaggctgtaa tttgtcccgt ctgaacttga atatttgcca tacgtgctat
    39961 ttgcgttgtc gaagctacgg ctccattgaa aattctgcga aacagcagca ttcagcgatg
    40021 gaatacgcat agcttttgcc tgattggcat taatccggtt ggtttcgttg gtcaaagcct
    40081 tttgctgaat gctgatattt ttttgcattg cataatcaat gcatttgggc agcgtccaaa
    40141 ccgaatctgt tacctgtcct tccaacggca atcccatcag ggcaagtaac aataaactgt
    40201 ttattttcca tgaaattttc atcatattac tttaatttga acccaaaagt accttcacca
    40261 cttatgctgc ttcaaatgaa atagacgttt gggctttttt taccgacaga aaaggaaaat
    40321 ttctgccttt tgcagatagc aggcaactca aaatttatta ttgaaaggat tatttagtat
    40381 tgctgataaa ctttttatag atttgataaa aaaacgtatt cactacattt taagacctta
    40441 aaaaatcatt gtgatggaca aatacagcat tactattgat acatacaaca agtcggctgt
    40501 tagcttccag gacaagttta tgaacatgga cttgtacaac gctacctacg acaagttttg
    40561 ccaaaaggta gaaaaaaagc atgcaagcat cctcgaaatt gcctgtggac ctgggaacat
    40621 taccaaatac attgccgcaa aacgtcctga tttcagtata tttggcattg acctggctga
    40681 caaaatgatt gagctggcca gggtcaacgt accttctgcc cgctttgaag tgatggactg
    40741 cagggacatc ctgaaaatca ataatacctt tgatgccatc atgtgcggtt tttgccttcc
    40801 atacctcacc aaagaggaag ctacaaagct cattagtgat gcgtttcaac tgctaaatgc
    40861 tggcggagtg ctttacataa gcgctatgga aggcgattac agcaagtcgg gatatgagac
    40921 taccagcttt agtaatggca accaggttta cgttcactat caccaggcag attttttgaa
    40981 agaaaaattt aaagaagcag gttttgttga cgttgaactg ataacccaac aatatcccga
    41041 gccagatggc tcttttacta ctgatatgat attttttgcc caaaaacctt gacaataagc
    41101 aattcaaaac ttaattacac atgaaacacc catgattcta tcgttcataa acacgaataa
    41161 ataaaatatg ggtgttctcc cgtcaaagac gggacaagct atcattccat taaaaaataa
    41221 aaatgatgaa aaagattatc ctgcttcttg ttttcttagc tttttttagt cacggatttt
    41281 cacaaacaat tcattcacca gacgaaattt tgaagataat gatggattca aaattctcgt
    41341 atgaaataaa aaaattagaa aagactattg aatgcaaaga ttattctgaa agattaatta
    41401 ctcatgatta ttatagggtt gtcagcgact ctggcctttt cacaaaagaa attaccataa
    41461 atgaaaaagc aaaaccatta tttgaaaaag ctgaatacta ttttcaaaaa aataaacttg
    41521 acagtgcaat cacattttac caattatcat tgtcagctga tagttcaatt tatactgtga
    41581 tgacctatat tggccaggtt tttgaaaaaa ttggtgataa taataacgct attttgtggt
    41641 ataaaaaagc gattagtaag aactatattg actatatggc ccattggttt ttagctgatg
    41701 cgtatctgaa tgcaaataat ttgaaagatg ctgttgatga aattgtgatt gctcaaattt
    41761 taaacaggaa taacccaagg attaaaaagt ctttcactaa tatttttgag aaggcaaaaa
    41821 gaaacactga tgattggtgc ttcaatcctc aaattgagtt gatcaaagag tctgatacaa
    41881 aagcaagcgt ggcatttaat gaaaaatgga caggatacgc tatggcaaaa gcattatgga
    41941 cttttgaacc aggatataaa gaatcaatgg gagttaccaa tggatattct actcttgaag
    42001 ataaagaatg cctgatagcc ttacttattg cattagagaa tgcaaaaata tcaattaatg
    42061 acgagcctca actaaagatt ttaaaggatg ccgcaatgaa taaatacctg caggaatata
    42121 ttatttatga gattgtattg ccacaatctc catttgtagc atatcaattg cctgaacaaa
    42181 ctattttgaa tataaaaaat tacatcttaa attttagaaa cacaatgaaa taaccatgtc
    42241 aaacaacctg caatacaaat gaaaaatcta tcaaaaaact atctttggga aaagtaataa
    42301 caggatatga gttttgagat tttgacatca gaattattgg tcgagtttac cctaaaagta
    42361 aaccaatccc ctgttactaa gcttaaccaa atgaagaagc tggaattacc tgttgattat
    42421 tttcaatttt ataattctgt atcttctgtt tattcctcaa aaattgaagg cgaagacatc
    42481 gattttgaca gctatttcaa gcacaaattc atgaaaattc agtttaaagc agattatact
    42541 gccaaagctg acgatttata ctcagcttat gaatttattg catcaaaaaa aattacgctc
    42601 gaaaatgtta aaaaggctca tgctatcctg agttcaaatt tattgcccaa aagccaacaa
    42661 ggagtgtttc gcaccaatcc tatgtttgtt atcaacagcg atgaccaaat tgaatatgta
    42721 gccgccagtg cggagcaagt tatttttgaa ttggagaaat tattcaggga catcggaatt
    42781 cttcaaaaca aagaactgaa ttcttacgaa atattctatt ttgcatcgtt gatccacttg
    42841 gtatttgtca aaatacaccc ttttcaagac ggaaacggaa gaactgcacg attgcttgaa
    42901 aaatggtttt taattgagaa attaggcgaa aaagcccatt ccatccaact cgaaaagaac
    42961 tattatcaaa aattgaaaga ctactacaat aatatcaaaa agcttgggct tgaatatgag
    43021 agcttggatt acaaaaaggg tatagatttc ttgttaatga ctgtacaatc aattaacgaa
    43081 gaataaaaaa accatactcg tggcatttgt taccaaatgg taattaattg cccagtacca
    43141 gcaatcggaa caaactatat ctcataaaag attggctact attgatgtat gagaaaaata
    43201 gtaaagggct ttaattttga tggaagcctt tgcccgtaag gcttcttgaa aaaacgaaaa
    43261 ccatgataaa aatccgcaac ctcactatcg acgacttgga tggcctgata aaactccttc
    43321 aacagctatg ggttgacaaa gagataaata aagaggccgt cagggtagtg atagaaaaag
    43381 gcttgcacag cgacaaccaa aaatatatct gtgccacaga tggcgaaaaa ctggcaggct
    43441 attgttcact gaccataaaa aaacaacctg tgggtatcgg ccaacctcgg caacgttgac
    43501 gagctggtgg ttgacagcca ataccgtaac caaggcatag gaaaattgct gcttaacgag
    43561 attgaagcca tagcccgtca gcacaaatgt gtaaagattg agcttgatag tgcctttcat
    43621 cgcaccgcag cacacgaatt ttatgagcgg ttgggtttta aaaaaagggc atatttgttt
    43681 atcaaggagt tgtaattttt tatatttttc aggcaatttt aatcattgct ttacaaaatt
    43741 atgacacccc ccgcgattat tggccatcaa aagaacagaa cagcgtttat aacgctggat
    43801 acaagcaaat gcagcgcatg ttggctttgc ctgtccgaat gccctgataa ggtaattgga
    43861 aaaaccagtg gaaacatgcc ccctcatgct tatattgctt tcccgaagca atgcaaaggc
    43921 tgtttgcaat gcgtaaaggt ttgtccaacc aatgctttta ccttgattaa taccttgcag
    43981 gtaaaaaaag acaaagaaaa aaagcggtta ataacccaat tagtagctta tatctttttg
    44041 tttacaataa gtttattgat gatactctcg ggattggttt tacaaataag ctatcatata
    44101 ggtgaacaaa aaaaatttca tgaaaatgct gaaaaacttt taacacctac tatgacatac
    44161 aagcaggtaa gggcaataga cgcaacacgt acagttgtag gcctcaatta tgctgattgg
    44221 tcattggtac ataaaatcgc aatttcttta tgtacattgc ttctcatgta tcatatattt
    44281 ttacaaagaa aatggtacag catgatattt gtccacaagc tttggggaaa aaatatagga
    44341 acactatccc tatcattgtt gttcatttta ttggcaataa caggttatat ttcttggttt
    44401 ggaggatttt ctgagctaac ccgaaaccaa cttatcgaaa tacacgacaa actgggcatt
    44461 ctattggttg tattgctggt atggcatgta gccaaaagaa taaaatggtt tgtgaatgcg
    44521 tatggcaaat tataccttta acggccatcg caaatattca aaactgtatg acaagctatt
    44581 cataaataag atgttaatac tggacaatat gaaatgagca agatcattca ttaaacacga
    44641 gcaaggatga agcccgattt gtcgtgacgg gtttttcctc ggaaagcccg acggggtccg
    44701 tctgacctta ttaaaaattt attcacgcat gaaaaatatt tgagattgaa attatagttg
    44761 gcagttttga aaaaataata aacagcttgg tttagtttta gccaaaaagc ctgatataat
    44821 atgatagaac tctgggaatg acaataacaa atgaatcgag aaaatggcta tttcttagct
    44881 tttagcgctt tttcgtaaag cataaaacac caggataatt ccagctacga taaacggaat
    44941 acttagtatt tgtcccatca tcaaaggcat gcctttttca aagtcttcct gcggattttt
    45001 aataaactcg atgaggaacc ttgccgaaaa caataatatc aggaagatac caaaagccag
    45061 gccgcgcttg ttaaacaacc attctttggt atagctgtaa aaaagaaaac caaatatagc
    45121 aaggtaactt agtgcttcat atatctgggt tggatgttta gggagtattt cattctcgcg
    45181 gacaaaaata aagccccagg gcatcgatgt cgggatgcca taaatctccg aattcatcag
    45241 gttgcctaac cttacacaca taccgcccag cgctaccaca ataccaatac ggtcgagcaa
    45301 ccacaaatag ggttgtttgt ttttgcgggc ccacagatat aaagcaacca gcataccaat
    45361 ggccgaacca tgactggcaa gccccccttc ccaaattgcc agtattttca atggatgggc
    45421 aaagtaataa ccggggtcgt aaaacaaaac atgccccaaa cgggctccta ttatggtacc
    45481 aatagccagg taaacagtca actggtcaag catttcaatg gtatgccctt ctttgttaaa
    45541 aatcttttta aatacgatgt accccaatac aaaagccatt gcaaaaagaa gcccgtacca
    45601 gcggacctga aaaggcccta actcaaaaat taccgggtta acatcccatt gaataaagtt
    45661 tagcaccata gtgttttatt ttaaatatca aaagtaacaa acactggcat ataatccata
    45721 caacggcata gttaattttt gtgaacttaa cccaaattaa tcattaggag gctttttaaa
    45781 gccgaactat ctgatttagt tgggttaatc cttctgtctt aaacgggata agctaaataa
    45841 aaacgggttg atgcaattaa atagctggat ttcattcatt taattaatta aacttcctaa
    45901 tgtttaattt aggtcgaaaa aagtcttctt gcgcagggtt atttttttgc ttttctgaca
    45961 ttaatagtaa actttcaaaa taaatgggga aaaaaaatgt ttcagaacgt tggaaggttt
    46021 taaaacttcc taatttggat ataactgaaa aatacgctat ttcggaccat ggcagggtca
    46081 aaagttttaa atgccatgaa aaagggaaaa ttatcaattg ttctgttatc aggggctata
    46141 aggctattgt ggtcaagctt tcgtcaggca aatacacttc tttatatgta cataagctgg
    46201 tagccgaaat gttcctcgaa cgggcgtttt tactacaaaa atatgttatc cataaagact
    46261 atgataaatc aaacaaccat tttcaaaatc tttgttgggt gacatttcaa accatgaatg
    46321 cccatcaaaa actaaaccct aacaataaag ttctgaaggt caatcatgcc aaactctctg
    46381 aagaagaggt caaaactata aaattgaaat tgaaaaaaaa tgtcaaagca ttgattttgg
    46441 caaaaaaata cggcatcacc catactcagg tttaccgcat aaaaaatgga cagaattgga
    46501 aacatgtaaa tgtaggttga aatacccccc cttatttatt tcataaagtt ttagttagta
    46561 aaagcaagag cctgtcagga aaatccctaa caggcttagt tttattatta gtcagcccaa
    46621 caaaagaata actttgccaa tttacggcag gccttgaatt tttgtaactt ttgcttcagg
    46681 gcaaaagttt gcaatgcctg taaatataaa tacccaagcg tttttcttat gccgggctga
    46741 ccttatttaa ggcttttgtt acaaaccaaa ttcttttttc agcacgtcaa cataatccaa
    46801 tgattcccat ccaaaatatt tcactttttt gaatatctgt tcgtgcccat ttgtttgtcg
    46861 ggtataagtt tcgctgtctt tgtaaaatac ttctacttct gcttcaaaag gttcgcgccc
    46921 aaagtggccg tatgaagcag tttcgaggaa gatagggttt ttaaggccaa aacgttttac
    46981 aatggcaaaa ggcctgttgt caaatatttt ggcaattttt tcggctatct ggccatcaga
    47041 cagttttact tttgaagtac cataagtgtt gataaacaag cttacaggct gtgctacgcc
    47101 aatagcataa gccaactgga ctaatacctc gtcggcaata cctgcagcaa ccatgttttt
    47161 agcaatatga cgagcagcat aagcagcaga acggtccact ttcgaagagt ctttacctga
    47221 aaaagccccg ccaccatgag ctcctttgcc tccataagtg tcaacaataa ttttacgccc
    47281 tgtcaaacca gtgtcaccgt gcgggccgcc aataacaaac ttaccggttg ggttaacgtg
    47341 aaggatgtaa tcatttttga acagcccttt aatcctttcg ggaagttgtt tcataatgcg
    47401 cgggataagg atgttttgta catcgttttt gatcaccatt tgcatttcct tttcggcttc
    47461 atgtttgtct tcagggtgat tggttttggg cagtacaaat tcatcgtgtt gtgtagaaac
    47521 cacaatcgta tgaattctta caggcttgtt gttgtcatcg tattggaccg tcacttgcga
    47581 ttttgagtcg ggcctgaggt aagtcatttc tttgccttcg cgccttattg cagccagttc
    47641 gcgcaataac aagtgcgaaa ggtcaagtga aagcggcatg tagttctcgg tctcgttagt
    47701 agcataacca aacatcatac cctggtctcc ggccccctgg tcctcttctt tgatacgtac
    47761 aactcccatg ttgatatcag ggctttgctc gtgaatggca gaaatcacac cgcacgaatt
    47821 gctttcaaac atatattcgc ctttgttgta gccaattctt tcaatcactt tgcgggctac
    47881 ctgttgaacg tcaacatagc cttcggtttt tacttcgcca cctaaaacta caagcccggt
    47941 agtgcataag gtctcgcatg ctacttttga gttggggtcg ctttttaaaa attcgtcgag
    48001 gagggcatct gaaatttggt cagatacttt gtccggatgt ccttccgata ctgattcgga
    48061 tgtaaataaa tatgccataa tcttttaaaa attttgttta aaacaattgt ttttgggaaa
    48121 tgttgtaatg gcagtttgca agtgtataaa tgcggcagga aggaagtcca tatttagcat
    48181 tttttacagt ggttgcaagc agacacaaat ttttccacat ttgggcacaa aaatacacat
    48241 ttttgtgaaa agtacaattg ttatttttat ttgcttaata aacagttgtt tataaattta
    48301 ttcggacatt ttggcatgca aaattttcaa aaattgccat tttgtctaac gcttaaattt
    48361 tgctttaatt tgtttatatc tctgattatc aggtttatat atttttattt aaaattttgg
    48421 tatataattt gacaaaacct atactgaaat caaaataaaa agttttcgat aaaatttaaa
    48481 atgttgaact aaaataatgg aggatttagc catgttagca ctcagaaatt taaacagttt
    48541 gcccagcttt gttgaagaat ttttcaacca cgaattgcct tatagctgga accgcaattg
    48601 ggctaccatc agtacccctg cggtaaatat tgtcgaaagc aaagatgagt ttcgtattga
    48661 agtagctgcc ccgggcctta ccaaagaaga tttcaagatt aaccttgaaa atgaccagtt
    48721 gaccatttcg gccaataaag aagtgaagaa agaagaaaac gaagaaactt acacccgcag
    48781 ggaattttcg tatagctcct tcagccgctc atttactttg cccgaaactg ttgatagcga
    48841 aaaaattaaa gcttctcatc aagacggggt attgaccatt catgtgccca aaaaagaaga
    48901 agccaaacct aaaccagcca gagaaattgc tgttgcataa taatttggca ggtagttgga
    48961 ctaaactttc caggttttta aaacctggaa agttttttta ttgacatagc tgaaaaaatt
    49021 ctttcagctc atctttcttt tctttgctca acaaagcaga tttcacgccc tggatagccc
    49081 cttcaataga atagaggttg tttaaactgg ctttggggtc tatttcttta atcagcaaaa
    49141 aagctttttc gctgcccagc acttttagtt ttttttcgtc ctcaatgcct gctttttcaa
    49201 gcttctcggc caaggtttcg cctatattgg gcaatgttgt caatttgctc atttttacta
    49261 aatgttttga atttcattta tttaacgctt acaaaattat agcaaaagtg atgaatagaa
    49321 tttattaaat tgacgaatgt gtagaaaaag ctatttttga gaaaaatata acccttaaaa
    49381 atttgatatg agcaattttg acgagataat caaccggaaa aacacccaat gcctcaagta
    49441 tgacgctctt tttgaaagat ttggaaatac aaaattgtta ccaatgtggg ttgcagatat
    49501 ggatttcagg gtaagccagc ccataacaga agccataatg aatgttgcaa aacatggcat
    49561 ttatggctac tctgttgaaa gccaaaaata ctatgatgcg gtaaaatatt ggtttgaaaa
    49621 taaacaccaa tggcaactcg atgatgacag catatttttt accaatggcg ttgtttcggc
    49681 tattcacaac ttaatcaatg cttttacttc taaaggcgac ggagtgctta tccagtcgcc
    49741 ggtgtattac ccttttttta gcctggtaaa atctaacgac cgaaaactgt tagtaaacca
    49801 gttgattgag ctcaataacc aatattttat cgattttgat gattttgaaa agaaagcccg
    49861 cgaggcctcg atgtttatct tgtgcagccc gcataacccc gtcagcaggg tatggaaaag
    49921 agacgagctt gaacgtatgg cccaaatttg ttatgacaac aatgtcatta tcatttcgga
    49981 cgaaatccat aacgatttgg ttcagaaacc gtttaagcat atcccgacag caaccattaa
    50041 tactttgatc gaagatatca ccattacatg tcattcggcc agcaaaactt tcaacctggc
    50101 aggcctcaat acagcttata taatcacaaa aaaccgtaag tggaaacagc aaatacagca
    50161 gcactatgcc aggcagcatg cccatgcatt caatattttc ggggcagaag ccatgtatgc
    50221 tgcttatatg cattcggacc aatggtatag ccaattgttg caatacatta aagacaatta
    50281 cttgtatctg gtacagtata tccaagccaa tatcccacat gtaaaaatta cgcctatgga
    50341 agctacatat cttatttggc ttgattttag ggctttcaat atgactgacc aacagttgaa
    50401 aaattttatc atcgacaatg cagctttggc tttgaacgat ggcccgtctt ttggtattgg
    50461 gggcagcggt tttcaacgga ttaacatagc ctgtccccgc aaaacccttg aaatagcttt
    50521 aatacagctt acaaaagctt gtaattcatt ataatattga atggtatgta ataaaaaaat
    50581 actaaacatt cttgtatatt aattttcaat ggattaaatt taatctaaaa tttataccta
    50641 tgatttttga tattcccaat ttgagagaaa aacgcctgat ccaatgggca aagataaaca
    50701 gggtaaacat acctcatgac ccggctcagc tggtcaatgt aacgtcttta gatatcagtt
    50761 tcaaaaatct caaagctctt cctaaagaaa taggttgctt ggtaaatttg gaagaaatgg
    50821 atgcctctta taatcagatt gccgatttgc cgtgggaatt tgcgcaactc aagaatcttg
    50881 tcaggctgaa tctgagcaat aaccttttta gtgatatccc gggtattgta tgtaaccatg
    50941 ttcaattaga atcactcaat attgaaggca attttattaa aaaaattacg cctgttattg
    51001 ctaatctttc cagcctggtt aacttttcaa taggttataa ccagattttt gaattgccta
    51061 tagagtttgg gcatttaaag catgttgtta accttgattt atcatgtaat gcattgcatg
    51121 agctcccttc ttcatttgtt aagttgtata acctgaaaaa cctgaaaatt ggaggcaacc
    51181 agtttgcaca aaaacctgcc ctgcttgctg aaatgcccaa tcttgaaaca gtggacgaga
    51241 gtaaagacat tgaaataatt aacaaagcat ttttattttc tgttatttct gatagtaccc
    51301 gcagggtaga aaaatatatt gctttagggg ctgatgtaaa ttaccagttg aaggactatc
    51361 aaaacacacc ttttactact gctctatttg aagctcaatc attagaaatg gtaaaaatac
    51421 tgttggctta tggagccaat gctgctgtcc agcggcaata caaaatgact gtcaaaagct
    51481 ttttcgggca aaaagaaaaa caggttacag aaacatttct taccaaaaaa cacaagccag
    51541 aaatagaaaa atacctcaaa agtattgaga tgatttagtg tatccttttg gtttaataac
    51601 ttattgttaa aaatattcct caaaaaaaac gctttggcct tttggcttta gccaataaag
    51661 ctttttttaa cactattttc gtcaggccaa aagcaacaat tgatgatgtt atccttattt
    51721 caaacgcacg ttaatttatt aaattgctga atgacaattt cttaatcgat taagtgtttt
    51781 ttcaatactt atttgggtta aattaaaaat ttttactttt cgttcattgt ttgctaaaaa
    51841 aagttcaact tattgctatt tgggcgcttt ttaactcctg cttaacagat aaaccaacag
    51901 atatttttac tttagccaat ttacattgac tataaattta acacacattt gaacactttg
    51961 tataactagc tatgaaaaac ttttttaaaa aaatttttaa cgagaagttc agtcttcccg
    52021 caaaaataag ctttgtgctg ataggcttag cttctaccat ttggtttttg gtacgcgtaa
    52081 taccaaagcc ccaacgtgct gcata
//
LOCUS       DAOSPF010000019        51533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_702_length_51533_cov_6.794106, whole genome shotgun sequence.
ACCESSION   DAOSPF010000019 DAOSPF010000000
VERSION     DAOSPF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 51533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_702_length_51533_cov_6.794106"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..4731)
                     /locus_tag="PK252_05565"
     CDS             complement(127..4731)
                     /locus_tag="PK252_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14214.1"
                     /translation="MSVVRKTLVCLLSVVSLICCFTAKASEQSFKQFSGKDTLFTNEL
                     RLFMAKVEDDSALVQLGVFTALWDSAKISSENKKFILRTAIGLIDKKFRPFPDFMGYI
                     NVVIILNSKYPRHSKAWLEAMAEKVESKKVSATALRNVLANTIVFFKQRVLYKAPSLS
                     WLPSSPDFVVKNEERLIFFFPKTDLTCRAKRDSIMIKNTTGSYDPFENKWYGKGGLVT
                     WERAGYSVDSVSAILSGYTIDLSRTSYQADSVSFTNKSFFSAPMLGKLEDNVMYISKP
                     ENAQYPQFDSYIKEYNIKNIYPDVNYKGGVSMQGAKLIGSGTVYEPAKLYFFLHDTLK
                     LVAGSVTFGFKPHKVTASNAMVTIFLEKDSIYHGDVQFSYVSKNKELTLQKSDNYVAQ
                     SPFLNTYHNIDMNFDRMVWHIDEPFILLTSALGSSAGKAIFESVNYFKYEEFEALQYY
                     DDKHPLSQLKKCSQYYNGDRNFTAIDFANFIKRPMDQVRTLLFPLAVKGYIYYDPSTE
                     KVELKDRLFDALQSSIGRKDYDVLRFISNTSAPLENASLNLRNFDLTIHGIPAVFVSD
                     SQNVKIYPKARTIKLKHNRNFQFDGIVEAGLFTFFGRNFFFNYDSFKVSLGNVDSIRM
                     KVIVGYDLAMKPIYQDVNNVIQDVTGDILIDAPDNKSGVVNYPEYPIFTSRGNSYVYY
                     DHPSIFGGVYKRSRDFYFRIEPYVLDSLDNFSKEGMKFNGFLSSAKIFPNINEKLVLQ
                     PDFSLGFTHDVPDAGFPAYGGKGKFSRLLNLSNQGLRGKGHVKYLSATVTSEDIIFFP
                     DSMDAVDDFTIEAQAAPASFPNVEGKAIKVNWQPYKDVMYASNTANQFDMYSKQANFN
                     GLLSLTPKKLSGNGMINFPTADNSSKNFTFYQMLAHADTSCFRLKSSLDRQITVKTNN
                     VKSDINFATRIGDFKTNDTAVITEFPENRYIAKIDLLKWYMNKDELVMSSARKNVVPQ
                     EGKLYGFKDEPLIGARFVSTAPLQDSLWFVSGSAVYINKTKSIEASKVKYLDIADARI
                     FPNQEKIVIDSISKVQLLYKSKILANRETRFHNIYDATVDIASRKMYKASGKYDYIPV
                     TNIPEVISMHTITVDSANSTFTDGTIVEPDSFMLSPDFMYQGRAQIFAKEKYLTFNGS
                     TKIVVECPNTGTSWFDFKTVIDPTDVKIPVGDTLKEINDKKIVLGSYLRADTIMLYST
                     MFGTRKFHSDTSLIKSKGLLIFNKKLGNYEVASEEKLKNKSLPGQLITFNRKTCRFYG
                     EGKLKTGVNLGQVKLLNAGTIDHDLVANKVKLHMAMAVKFHFYQPSLEITRKYIDSLC
                     RDSIKIKTPYFRKLYGEFADTATIRKFFTEFDLPMLSEKEKENILPEAFKSTIFIDDI
                     NLVWDQATKSFRSEGKIGIGFIGGKPLHRYVNGYVEIWRKRSGDAMDIYLQVDEKNYF
                     YFGYTRGAMWVASSDKQFELPIREMSMKERSITVGRFQTPYSFVLSPQDKVVRVVRRW
                     KNGTTEEDLQLQNQDDINETPEIQIEEPQVTPNDSLQFNK"
     gene            complement(4707..5543)
                     /gene="prmA"
                     /locus_tag="PK252_05570"
     CDS             complement(4707..5543)
                     /gene="prmA"
                     /locus_tag="PK252_05570"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT14215.1"
                     /translation="MGYLEYNVTIEPFQPETSEALIALLGDYGFESFVENETGFQAYI
                     KEDEHKVSFFEKALSKLITRSEVRYTFKKIKDENWNAVWESNFEPVIIDTRIAVVAPF
                     HQINPDTKYVIKIEPKMSFGTGHHETTSQVMLMMLEVEMKGATVLDMGCGTGILAILA
                     HKLGAVDIVGIDNDPQAVENAIENFERNSCTEIIAITGDTGAIPSKMFDIILANINRN
                     TIADNVGEWMKYLKKGGYIIFSGFYEEDIIFLKAIFDKLNLSILKTTTNNKWACLLCE
                     KH"
     gene            5658..7529
                     /gene="yidC"
                     /locus_tag="PK252_05575"
     CDS             5658..7529
                     /gene="yidC"
                     /locus_tag="PK252_05575"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT14216.1"
                     /translation="MDKNSVIGIIIIGAILIVFSVWNTPNKKELEEARRKADSIALVQ
                     KLEAQQKQLEAKKDTVAAKALDAKSEAQIATEKYAEFGQAATGEAEFYTIENELVKLT
                     LSKKGGKPYSVELKQYKTFDKKPLILFDGDSNQFGFNFYTANNRLISTNELYFTPVQT
                     NKNVVLAEKDTAASISFRLNAGENKYIQYTYTLHPANYKIDFKVKFVNLDSIISSNTT
                     SIDLKWHVFLKAQEKEVPNEKTYTEVFYKFNQEDVESFKPSKENEKTETLRNKIKWVA
                     FKQQFFSSVLIADNNFENANLTIKNNTDTSRILSLMNAELGIAYQPGKTNEVGFAFYY
                     GPNHYQTLKKYDLDLEELVTLGSWIVKWINRFLIIPVFNFLNQFIGNYGIIILLLTIF
                     IKLLLFPLTYKSYLSMAKMRVLKPQIDEINEKIPADKPMERQQATMNLYRKVGVNPLG
                     GCLPMLLQFPILIAMFRFFPSSIELRQQGFLWASDLSSYDAIITWSGHIPLITEYFGN
                     HISLFNILMTITTLITIKMNDQANAQQQMPGMKVMMYIMPVIFMFVLNRFSAGLTYYY
                     FLANLITIAQNEIFRRSIDEAKLLHQLNENKKKPMKKSSFQARLEEAARKRGYNTKK"
     gene            7526..8233
                     /locus_tag="PK252_05580"
     CDS             7526..8233
                     /locus_tag="PK252_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT14217.1"
                     /translation="MMPVGRLFLIPTTLGSDNPLEVLPAYNVEVLKNISTFIVEDEKT
                     ARRFIKKIWREAIIDNMQFLLLNEHTKPEDTVHFLDKCTHEDIGLMSEAGVPCIADPG
                     NIIVNMAHAKGIEVVPLVGPSSIIMALMASGLNGQNFAFNGYLPIEKPARAKALKHLE
                     NRSASENQTQCFIEAPYRNNQLVADMLQCLSPATKLCIACDISLPSQFIKMQTIDQWR
                     KKVPDLHKRPAIFLISK"
     gene            complement(8267..9604)
                     /locus_tag="PK252_05585"
     CDS             complement(8267..9604)
                     /locus_tag="PK252_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT14218.1"
                     /translation="MAKEKKIFVLDTNVILHDYKCIYNFEENDIVVPIVVLEELDKLK
                     RGNDILNMHAREFTRELDALSGEGLCTSGIPLGKKLGKLFIITGKPFSKNLQASFPES
                     TPDHRILAIAEYIQESNKGQYSTTVLVTKDINLRVKAKSLGILAQDYKSDKVHDLETF
                     FHSIETIEKVDPKHISELYNLQDGIPLSNVTKKKVTDESAKYFIIRNEKTSVLARYNR
                     RTSNLEKVEKLRCYGIEPRNAEQTFSLNALMDPEIQLVALTGKAGTGKTLLALAAALQ
                     QEKDFETIYLTRPIVPLANRDLGFLPGDVNEKIGPYMQPLYDNLSFIKHKLKVQSKEA
                     AKIDEMQKNEKLQITPLAYIRGRSLSNAFFIVDEAQNLTPHEVKTIITRAGEGTKMVF
                     TGDIQQIDSPYLDSFSNGLSYLTNKMQGQEIFAHIHLLKGERSYLADLAGNLL"
     gene            9646..10974
                     /locus_tag="PK252_05590"
     CDS             9646..10974
                     /locus_tag="PK252_05590"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT14219.1"
                     /translation="MIHTYSEAIEYLYSQMPVFHRDGASAYKPGLERITKLCTILRLD
                     KLDFKTIHIAGTNGKGSVSHGLASVLQTSGYKTGLFTSPHLVDFTERVKVNGENIEKD
                     KVIQFINHSFEAIQEVQPSFFELTTALALWYFKIMQVEIAVIETGLGGKLDSTNIISP
                     ILSVITNISYDHIDLLGDTLEKIAIEKAGIIKPNVPLVVGQTQAETGPIFIDTAQKLN
                     SPVYFADKLLKMTNAKVTNEFKQKISVIANDLTMKEHEGDYLIDLQGVYQQKNILTML
                     TAIDQLKKQNITITHEQIKLGLSSIVEKTGLLGRWQVINQHPYTVLDTGHNEDGIRCV
                     VEQLNTIPHNKIHIIFGMVADKDINKVLALLPKNATYYFTKAPGPRALNEHLLQQQAK
                     QYNLQGNTFETVENAYKDASFNAQPNDIIYIGGSTYLVGRFLEIRKNMEE"
     gene            complement(11116..12384)
                     /gene="ilvA"
                     /locus_tag="PK252_05595"
     CDS             complement(11116..12384)
                     /gene="ilvA"
                     /locus_tag="PK252_05595"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF006390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOT14220.1"
                     /translation="MSALSQSSVKIKLNQVQKARERLRNVVTNTPLMHDVNLSERFDC
                     NILLKREDLQVVRSYKIRGAFNKISMLPLDILKKGIVCASAGNHAQGVAYSCRKLQIK
                     GHIFMPTPTPKQKIKQVEFFGREYVNIILAGDTYDDAFNAALKFKNEQKLEFIHPFDD
                     IHVIEGQATAGLDILSDTSSHIDYLFVPIGGGGLAAGVGSVFKTLSPDTKIIGVEPAG
                     AASMKESIEKGEVVTLQHIDKFVDGAAVQRVGDLTFEICRNIIDDIILVPEGKVCSVI
                     MQLYNEEAIVVEPAGALSIAALDFYREQIRGKNVVCIVSGSNNDITRTEEIKERSLLY
                     EGLKHYFIVKFPQRPGAFREFLENILGPNDDISYFQYSKKNNRELGPAVVGIELQKKE
                     DFEPLFQRLKASNFLQEYLNEKPDLFQFLI"
     gene            complement(12386..13474)
                     /gene="hydE"
                     /locus_tag="PK252_05600"
     CDS             complement(12386..13474)
                     /gene="hydE"
                     /locus_tag="PK252_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOT14221.1"
                     /translation="MQKIFSSILALESFEASDLKVLLNSEGEQKKALFEKANEIKQSI
                     IGNKVFFRGIIEYSNVCQKDCFYCGIRKSNKKIKRYTVSDPEVIDAALFAFKNRYGSI
                     VLQSGEMTSPAFVERIENLLRQIRLLTNGQLGVTLSMGEQSEETYQRWKNAGAHRYLL
                     RIESSNRQLYAQMHPNDAMHNFDRRLDCLKLLKKLGYQVGTGVMIGLPGQTIDNLVED
                     LLFMKELDIDMCGMGPYLEHEDTPMYEKREMLDSQKQRFDLALKMVATLRLLMKDINI
                     AATTALQAIDPLGREKALKCGANIIMPNITPAKYRTDYKLYENKPCTDEAADDCLSCL
                     DVRISLTGNKVGYGQWGDSQHFKNKKHL"
     gene            complement(13561..14343)
                     /locus_tag="PK252_05605"
     CDS             complement(13561..14343)
                     /locus_tag="PK252_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14222.1"
                     /translation="MLKKVIYLLIACGVAICAVFIRQFSDSDRPVVHYAFVGEKEYKL
                     KFPIVSEGKDECLVDFEIPDTSVTGKIYYRLANSSMAWKVQPMVRTGNTLVTMIPPFK
                     PNVKVIYYVELIKGGTAIAVAKDNPVWLRFQSTVPKYISYPYDFLYLLIVLLLIYVCI
                     LAIYNIRTYAKYLRASFYISVGVILFSFVIHLISFRHIFLAFDPHNNLTFYKDLVAFL
                     FLYFVFKMRQKPKARFLIIGIPLVVLLLYCIPQHIMFSMFYR"
     gene            complement(14412..15740)
                     /locus_tag="PK252_05610"
     CDS             complement(14412..15740)
                     /locus_tag="PK252_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT14223.1"
                     /translation="MKDLTVGHEGKLIWQFSVPMIIGNVFQQLYQIIDSVVVGHFIGK
                     EALAAIGASFPIIFVIVSLAIGVSMGGTVLVAQFFGAKDFQRLKRTVDTINVLLFFLS
                     MVLSTLGIIFSAQIFRMMHLPEPLIPIATSFLNVYLSGTFIFFGFNAISAILRGMGDS
                     KTPLYFLIISTIINTLLDLLFVGIFKWGVASTAVATIISQSVAFIAAIIYLNKTHKLL
                     KFSIRNIEWDRLIFSKIMRIGLPTGIQNTFVALAMMALMVIVNQYGTDVIAAYSVGYR
                     IEMFASMPAMNFAMALSAFVGQNIGAGKMERVKTGLLATLKMSVCVALCISVLVFFAG
                     DLLINMFTTDANVIRYGHEYLRIAGSFYAVFTAMFIFGGVMRGAGDTFIPMLVTLFSL
                     WFVRLPLASFLSGKYAEKGIWWSIPIAWFIGAIFSYFYYRTGRWKKKSFV"
     gene            complement(15811..>16791)
                     /locus_tag="PK252_05615"
     CDS             complement(15811..>16791)
                     /locus_tag="PK252_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOT14224.1"
                     /translation="KVEFGSQKVSFNRAEMGKVNLNFEGCEQKTGRFNFKKVLIQGGS
                     SNFSLVEMPDTEAVFSSSDIGMCTTSFHNSAFKSLFLQSCHFDCYIDLRVKKADFLDL
                     SDSIVRDIVDLMPYDWTVDIKSVNFEGMRLVGRMYLSWDKNRVKSMINNQPNLSNRSK
                     CEQFRVLKQNFNVTGQYTDEDKAYVEFRRHEMAALLDEMQQKSTLKSFFYYPVYWFKK
                     IVFDYMGLYATSPSRVLISVVVCWFLFGFGYFFIELLGVGKTMSGVGNPDNISVFAQS
                     FYHSAITFFTIGYGDVFPMGVSRLFSALEGFLGVFMMSYFTVAFVRKILR"
     assembly_gap    16792..16801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16802..17554)
                     /locus_tag="PK252_05620"
     CDS             complement(<16802..17554)
                     /locus_tag="PK252_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14225.1"
                     /translation="MQDFQLSIIDEPYTTSDGNGYRQTALLRSNEKHHNARKEIRLGY
                     ISAIELYRLIKEEKPIKLDSVFIDDFDLHQYRINEKLDIKKGIVLHDFTVTNSFLVSS
                     SDIDFTYAVFEGAEACFDNTVFVAPKINFANAHFRTENVSFSYCHFKVQRTDFSYSFF
                     KGNSLSFKNAVFIDGVKDFRYIDVEKANINFVNTDFGHGDVFFINAQFGTGDVSFKVA
                     TFGDGAKDFHYARFFGSDVSFERCRFGNGLIDF"
     gene            complement(17556..18359)
                     /locus_tag="PK252_05625"
     CDS             complement(17556..18359)
                     /locus_tag="PK252_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOT14226.1"
                     /translation="MPVITIISDWNRGDFYLSAMKGKIISNLPGVQLVDISHGISSFD
                     YVRAAYIMRNTYRTFPKGTVHIICVDSEKKPQSPHIVVKHDNHYFIGAYNDVMKHIFI
                     APAREVWVLEHDLNTVFADYEVFADVAVYLAQGGNVENLGTKWDITLEAHLQLPRIDS
                     NSMEGNPVYIDSYGNAITNISLEDFTRVGKNRPFELYVLNKRYTLNKISKGYYEAKDG
                     ELLALFNSSGWLELALYRGNLAEMFNLSNKTPVIINFFDKTEENQLILN"
     gene            18466..19422
                     /locus_tag="PK252_05630"
     CDS             18466..19422
                     /locus_tag="PK252_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT14227.1"
                     /translation="MTEKEIYIEEIDPGILFGVHNANMTLIREAFPMLKIMTRGNVIK
                     ILGDKYNIEAFEQKLAAIIDYFHQFHSLSKDEVDEILFATGRTSLKTSESNGVLIYSH
                     SGKAIKARTVNQHKFLKEYDKNDLIFAVGPAGTGKTYTAIALAVKALRDKHVKRLILT
                     RPAVEAGEKLGFLPGDMKEKLDPYLQPLYDALHDMMPMKRLTTYLEDGTVQIAPLAFM
                     RGRTLDNAFVILDEAQNATVNQLKMFLTRMGRDAKFIVTGDLTQIDLPRKSQSGLPFA
                     LKLLRNIDGISVVEFDQRDIIRHPLVKQIVDAYDKSDIHEND"
     gene            complement(19419..20114)
                     /locus_tag="PK252_05635"
     CDS             complement(19419..20114)
                     /locus_tag="PK252_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14228.1"
                     /translation="MDDKSKILEVLRSKSLTKQMIHDNTANVFTVLKTVLTEIAAEIN
                     HELSGLDQRILLEFKDNGKAEAQLRIAGDLLIFSMHSNVFEFNREHNIQKTSFAAQNP
                     LNTYCGIINIYNFLADSFKFNREEDLGYLIGRIFINRDFYYMVEGKGQLAFTCNMLGT
                     EKIDRAAIRKIVIGSIQYALDFDLLVPPYDQVKIATVAQMIENIENSKMQTGKRLGFV
                     FNSDDINGVKNHR"
     gene            complement(20127..21602)
                     /gene="proS"
                     /locus_tag="PK252_05640"
     CDS             complement(20127..21602)
                     /gene="proS"
                     /locus_tag="PK252_05640"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT14229.1"
                     /translation="MAKELTSRATDYSQWYNDLVIKADMAESSAVRGCMVIKPYGYSI
                     WEKMQASLDKMFKDTGHTNAYFPLFIPKSFFSKEASHVEGFAKECAVVTHYRLKNDPN
                     GKGVVVDSEAKLEEELIVRPTSETIIWNTYKGWIQSYRDLPILVNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATREEAEIETRKMIDVYADFAENYMAIPVIKGHKSANERFA
                     GALDTLCIEALMQDGKALQCGTSHFLGQNFAKAFDVKFQSKQGVQEYVWATSWGVSTR
                     LIGALIMAHSDDNGLVLPPKLAPLQVVIVPIYKTDEEFKKVAEKANEIFSMLKANGIS
                     VKFDDRDTHKPGWKFNEYELKGVPVRIAIGPKDLENGTVEIARRDTLEKKSYAATEIL
                     DTVKFLLGDIQKNLYNKALAYRKANTTEVNSWEEFKKVLEEKGGFISAHWDGTSETEE
                     KIKEETKATIRVIPFDQKKEGGKCVYTGKPSEGRVLFAKAY"
     gene            complement(21852..22637)
                     /locus_tag="PK252_05645"
     CDS             complement(21852..22637)
                     /locus_tag="PK252_05645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT14230.1"
                     /translation="MLLIYRIFSAIGFLYHSKPLRVNSFGLPFLIKLTDGFLLNAKNV
                     QKNLAPVELLREELLSDKTLIHIIDPGTGVVRYDTISGICKKASGSARKLRILHQLVK
                     FVEPQMAIELGTSLGLSSASIALAMPYKTVITVEGEKNLAEKANDNFVKLGLKNVRLI
                     SQKFDDVLPVLVDNLKSPFFAYVDGNHTYKATVKYYEMFSQHLDEHSMLVFDDICWSK
                     DMGKAWDYICNHPKSTICIDFYDIGVVVFQRGLIKQKINLRVY"
     gene            complement(22640..23362)
                     /locus_tag="PK252_05650"
     CDS             complement(22640..23362)
                     /locus_tag="PK252_05650"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOT14231.1"
                     /translation="MKKYLYCLLVLFFVFASCEKKTIKGNGKVTEQTRLLEAFNQVSI
                     MGAFDVVLVNSDSSRLRIIADENLIENVVAIVEDSALKISNIKTVIGSRELKLYIYAP
                     NIESVDFSGATELSSDSLLSWDALALNITGAGKVDLQIDVKKFSVNVSGGAEITLKGK
                     TDELALNIAGTGSLNAFSLKIGQCDIDISGFGRAYVDVEKMLRVNISGFGKVEYNGAP
                     EIRQNITGSAKIIKAGTEPKDK"
     gene            23754..27296
                     /locus_tag="PK252_05655"
     CDS             23754..27296
                     /locus_tag="PK252_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14232.1"
                     /translation="MTIKTKYLVKALTLIMGILLQFQMLEAAPKDQFRFEGSLKSSTE
                     GVADMIINIFENNKKINSIKSNSQGNFALELEHNKQYTLELIKTGFIIQKIVIDSKIS
                     PKLQASGGIDLAIDNEIPVFENVEGLKTDIFEQPVIYLIYNENKSYFEQDRKRSKNLS
                     ALTNQIVSHKNKLANNELKNANNLFNKKKYPEAIESYLKVLSISPNNQQASLKIKEIE
                     KITKDEQALENKYNKTIKEADALLKQADYYEAKEKYKIAVAIYPEKKYAKSKVSEIDS
                     IISSNLSKNLTLFEKLKKDALKAYQNKDLENSLALYNEAKKLNGEDKQVIKQIAAISK
                     AIEAEKLRKEEELRQLEEKFTSFLNKAKDYEKNKDWNNSLSMISQALLIKPDDAECNK
                     IKDNAEKNIAIEKQRQQKEKNYQDTLALADKAAQDKNYHQAIKLYEYAQKMKPGEKYS
                     SERINKIKTFLKELEASKQVSPLSKNIAINDSLSNEKYKNAEDYEKKLKQLSFTKDKK
                     HYMQTIARLSIMYQQQRKLDKGIRLLEKSLLEAKRNKDIESITEILNYLTVFYYDSGM
                     YKQTIDAYNQMIDIFKQTGNLEMLAHTLSEAGAVLASVYQYDAAIETLEKALELHKKN
                     DDKKMALLTCQYIAEVYANMEDSKNAIKYFKQAIDRAHSMNDQQALGTLQNSIGVVYC
                     KSGNYDMALTYYNQALEVEKKANNMKNMSLTLNNIGNINYNWNEYEKAIEYYQKSLKI
                     KREINFEDGIAITLYNIGSTYIELKNLSKALDYLNSSLEVANKTKFGEVIQQSYKALS
                     KVYEMKDDFRNAVAAYKSYTDNAIPGYNNEKQFYETNDFYGKDRQLVTKLKKELYKQK
                     ILIENQALINKQKEQQIAIQKMEISQQKSKTAKTRTLLLFSILISLLIGSITIQIFRR
                     YKEKKLYSEVISFQKRQITDSIEYASRIQKAVLPPDEVVKAQFPNSFIYNKPKDIVSG
                     DYFYVTAIDNKVYVAVADCTGHGVPGAFMSMLGLSLIKEVIHFSNEAPSANTVLNNLR
                     DALMKSLHQTGREDEARDGIDIALCVIDKNTNILEYSGANNPCYIIRDNKIIDLKADR
                     MPIGIHPILNDFNSQQLELKKNDVIYLFSDGYRDQIGEETLKKFRKDYFNNLLLEIHA
                     LPMDQQAYILDQRHLQWRGSIEQTDDILIMGIKV"
     gene            27349..27873
                     /locus_tag="PK252_05660"
     CDS             27349..27873
                     /locus_tag="PK252_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT14233.1"
                     /translation="MTLPSGEYTQEELNAFKGIFDKYYESIRNFAYFKTGDIDVAEDI
                     VQETFIKVWEIRDTLVEDTVKSLLYAIAANSIKNHFRHKKVVLNFAYSKNNESENSES
                     ADADLQQQEMHNKLLFILNEMPEKSKEVFLLNRIEKLTYQEIAKRLDLSVKTIEKRMH
                     QALVHIRKHLNYKI"
     gene            27873..28739
                     /locus_tag="PK252_05665"
     CDS             27873..28739
                     /locus_tag="PK252_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT14234.1"
                     /translation="MATDNDILKQFGLESDIEIQRFLESVDKLKVPVSSKNRDEAWSK
                     LENSIKTRKTKEIKFNFKHAFLIAASIALFIGLWFGFEQWNHAKYSTSSGKTLTFELP
                     DGSQVILNAESQVNWNKFRWKYSRIVILKGEALFKVNKGSRFEVQTSQNKVSVMGTKF
                     NVFSRPGYFEVKCYEGKVAVDIKGSSQVILTKGKAIKKESSSSTAESFEIQESDTNNW
                     TKGEFYYSNASLRLVLDEIERQFNVNINYTAEDRRYSGYFKNNSLDSALINICMPLGL
                     KYNIIRDSVVIR"
     gene            28795..31482
                     /locus_tag="PK252_05670"
     CDS             28795..31482
                     /locus_tag="PK252_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14235.1"
                     /translation="MFKWLTRYLLLFLIIPFSINTYGQKVSADYSNLSLTEVLTAISQ
                     QYHIKIAFDTQLADKIAITQSLKQIPIEKALSLILNNSGLKVQRMGDVYMVIPDLNPV
                     KRVPDGNSVQQTATTKTRKFIYGAIKDMQSGEALPFASIYLKNNHQGITSNADGIFKL
                     ETKDEDSIYLVINYIGYEPFAIKAKASSHPELLQIRLNPQLQQLQAIVISPKIEVFEN
                     RGTQPDVIKFCPSKLENIPSINELDISTPLQMLPGISGCTENAGGFSIRKAPTDKTLM
                     VFDGFTLYHMNHFFGAFSAINTKSIKDIQVRKIPGDANLGGSSAGLIEITGKSGNMQD
                     ISVNAGIDMLATDVEIEIPIIKNKCSFLFAGRRSFTDKFRTPLYNAMFDNARYEFLSY
                     YRKPPVAFSSGTGDPQYYFGDFNSKISINTSEKSTLSLSAFGSKDDLNFSQLAAYPKI
                     KENTNWQTKGFSIRWAGDVNTSWNTELVSGYSNTTFDYTLSDSIQKFRKRLVRTVEYI
                     VSKGNNIDLYLKNFSIQNNNKINTSEKGQLIFGAGMQVVSSYYGLNAFAWVNSMNILD
                     TSRIYKENSKITSAWLQYEWKNEYLTIKPGLRVNHYKLLNKNYPELRGTMFIRPVNKL
                     ILRVEAGNFYQFVNKVDFVRHGDFRSAWILSDGEKYPVASSKNIALGVNYNITSRLNI
                     DIEAYYNQMFDLTSSQTIYKITTNNNIKLANISWLYDNKVKGLDVLLKQDWQYYQIWL
                     SYTLSEAKNKAPQNSTTQTYTADNDQMHEVKLLQLFNYKNFSISWSNIGGSGIVWDEY
                     ILDNNLQLTPDYQKNSGSAPFYYRMDAGANYTLKLSKCSLKAGINLFNILDRKNLINK
                     FEKLSDTPIADINQGLNPLEETSVYGLGVACNFFLNISF"
     gene            31590..32450
                     /locus_tag="PK252_05675"
     CDS             31590..32450
                     /locus_tag="PK252_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT14236.1"
                     /translation="MSSEGKKYKHKDIKAFDETLKLYENFKIPAKYSKDDSWEKFKTV
                     IETPHEKAPPKTRSLKVFYTSAAAIILLLIGLSLFNYFNANIIITSKNGENKLVVLPD
                     SSTVELNAASTLSYNQFGWKHHRLVILDGEGFFSVKKGKRFEVKTNHGNVVVLGTSFN
                     VFARNENFFVVCKTGKVKVESINTVVLTPGLKAFKNKDQKINIENAIVEHETAWQNGE
                     FWFNNVRLIDVLNELERQFDIKIVYKDIENRRYTGYFNIKSLQDALTAVCSPMQLKFK
                     NENDKTYIIY"
     gene            32469..33299
                     /locus_tag="PK252_05680"
     CDS             32469..33299
                     /locus_tag="PK252_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14237.1"
                     /translation="MRKMNYLTMMALVAITINFTSCKKDDDTQVSITNEDVTVMQDDA
                     QINDMMDDIDNEADEISDVAQSKSALASDSTTYSGREVDRKLNNDGTRTITITYTNFQ
                     HPKAKNERIKNGIVYIVVTGSRSDNTYKRIVTFENFTINNNKIEGTKTIEKMETLKYK
                     ITLTNGKITFTDGSSVTCNYIRYRTMVAGIATPLYIWDDAYTFEGSASGVTSKNIEYT
                     KTITKAVKIYTAYRYPVEGTFSFTSGDKTFIFDYGDGTKDNLATITYKDVSKEITLKK
                     "
     gene            33557..33934
                     /locus_tag="PK252_05685"
     CDS             33557..33934
                     /locus_tag="PK252_05685"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOT14238.1"
                     /translation="MKEKIELEYILKTSPKVLFPRLSTATGLAEWFADNVTVKNKTQY
                     AFHWDKSIEEAEQTYIRENVVVKYEWKNKDEKAFFEFRIRIDDLTGEMALIVTDMVDS
                     DEKDEIIDLWDIQINKLKHVLGL"
     gene            33975..35483
                     /locus_tag="PK252_05690"
     CDS             33975..35483
                     /locus_tag="PK252_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT14239.1"
                     /translation="MRIKKLHIFILKSFISPLFVTFFIVIFILMMQFLWKYVDDLVGK
                     GLGYDVLLELLFFASATFVPLALPLALLLSAIMTFGNLGENFELTALKASGISLWRIM
                     YPLILLIAAISIAAFFYSNISLPYFNWKFRALLFDIQQQRPELQIKEGVYYNGIQGYV
                     IKIGDKDVRTNQLKNIKIYDHTQNDGNTSVTTADSGYIKITPDKRFLVLTLLHGQSYV
                     DMPEKNRSEQDRKYPFRRDKFSKQEVNIPLEGFNFQRTEEALFRNNFQMMNLKQLNQA
                     VDSMNNEIKADANMLKSNMMYGHLTSSLSPPKDTSFKLDSTIKFNNVRFIYYLLDKNE
                     RKAIINQALADARNAKNNINSETEVRRNKMIRLRKHEIEWHKKFTLSLACIIFFFIGA
                     PLGAIIRRGGLGLPLVISVVFFIIYYIVSLTGEKMARESLWPVWQGVWFSSLILMPIG
                     IFLTIKATNDSGIFKVEVYQNTIIKFFKQVWSYLKPLYISISNVLSKKTDNR"
     gene            35520..36716
                     /locus_tag="PK252_05695"
     CDS             35520..36716
                     /locus_tag="PK252_05695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT14240.1"
                     /translation="MKILILSNKPPYPPKDGGAIAIFNLAKGLADAGHFVHILSMNTS
                     KHQVKPETMPLFSNIRFSFVNIDTSIQPVKALFNLLFSSIPYNAERFISKYFLSQLKT
                     ILQADQYNVVQLEGPYLGYCIPLIRKYSNAKIVIRAHNIECEIWERSALQEKNILKKL
                     YLSILSKRLKLYEKNILQQCDIILPISSRDNEHLKNIGICKPGIVVPVGIDINNNYNH
                     HNSSENSILYIGALDWMPNQEGILWFINDVWPIIQKKEKNVILHIAGRNAPEWLVKKL
                     SSVKNLLFYGEIENSHNFIKQHGIMIVPLFSGSGMRVKIIEGMALAKAIVTTPVGCEG
                     IDAENGKHLFITDKAETYAEHVLYLLHNNAKAEEMGNAAFEFVGKEYSNQSIISKLTN
                     FYNQTL"
     gene            36716..37894
                     /locus_tag="PK252_05700"
     CDS             36716..37894
                     /locus_tag="PK252_05700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT14241.1"
                     /translation="MTAVAVIFWLCVLAVFYSYVLYPLILQLIVFIKGRKTYKSYEKN
                     EQLPSISILIAAFNEELVIEQKLRSIFNTTYPKNKIEVWIGSDNSTDNTNAILEKLSN
                     EYSNLHFINFHNRQGKISIINQLSDKANGAILIISDANVIFEENTLFELTRFFADEQI
                     GLVDTHMKNYGFKRDGISIQEKSYISYEVKIKNLESSSFGTMIGPFGGCYAVRKELFT
                     KVPINFMVDDFFVCMKVLISGKDAINNMNAIVYEDVSNNLNVEFRRKIRIAAGNFQNL
                     RYFKHLILTVYKGVSYCFISHKVLRWLGPFFIIGAFISNLILALNAKFYLILFLIQLM
                     VSILPLVDFLLKKINLHIVILRFATHFYTMNLSLLLGFIKSIKGIKSNVWEPTQRLQG
                     "
     gene            37860..39116
                     /locus_tag="PK252_05705"
     CDS             37860..39116
                     /locus_tag="PK252_05705"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOT14242.1"
                     /translation="MSGNQHNDFKGKLSTIPEAIEDIKNGKIIIVVDNEDRENEGDFI
                     CAAELITPEIINFMATHGRGLICTPLPEERCDELELDLMVNKNTATYSTAFTVSVDLL
                     GYGCTTGISAYDRAMTVKALVNPETRPEELGRPGHIFPLRAKNKGVLRRPGHTEAAVD
                     LTILAGLKPGGVLVEIMNEDGTMARLPELVKIAEKFDLKIISIKDLIAYRLEHESLVI
                     GGEKVKLPTAYGDFELIPFIQKSNGQEHVAIIKGSWTKDTPVLVRVHSSCITGDIFGS
                     KRCDCGDQLHHAMQMIEKEGTGVLLYLNQEGRGIGLFNKMKAYKLQEQGMDTVEANLF
                     LGFEDDERDYGIGANILRELGLGKLRLITNNPKKRIGLEGYGLEVVENIPIVVSPNPH
                     NLKYLTTKEEKMGHFLNLARYRKDSF"
     gene            39132..40088
                     /gene="fmt"
                     /locus_tag="PK252_05710"
     CDS             39132..40088
                     /gene="fmt"
                     /locus_tag="PK252_05710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT14243.1"
                     /translation="MGLKVVFMGTPDFAVATLDAIVKSKHKVLAVVTSPDKPAGRGLQ
                     LQMSEVKQYALQQSIPVLQPEKLKDESFIEKLKSFNADVFVVVAFRMLPEVVWSIPRK
                     GTINVHASLLPQYRGAAPINRAIMNGETVTGVTTFFIEKEIDTGQIIKQNKVNIEPTE
                     NASSLYEKLKIAGANLLIETLDGLENNTLKPIGQHILIDKKIPLNEAPKIFKEDCKID
                     WNKSKEAIFNHIRGLSMYPAAWAILAGPKTATVKIYTASMVESVHNLPNGTIETNNRS
                     YLHVAVNNGMIAIETLQLEGKKRLNVDEFLRGFQHIESYHFE"
     gene            40100..40849
                     /locus_tag="PK252_05715"
     CDS             40100..40849
                     /locus_tag="PK252_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT14244.1"
                     /translation="MMHTILNHRTIRKYKTTPVSQKMLEEILLAGTKASNTGNMQTYS
                     MIVTTDSTLKEQLCKCHFGQEMVKVAPVLITFCADFNRFNKWCNLRKAKPGYDNFLSF
                     FNAAIDALLVAQNVALAAEEKGLGICYLGTTTYNAKQIIDILNLPKGVVPVTTIVMGY
                     PEVIPDLTDRLPLEAVVHYEKYQDYSDEKINELYSEKEALPFHQELLKINNKETLAQI
                     FTDIRYKKADNIAFSKAFLKAIEDQMFMNNE"
     gene            40855..41820
                     /locus_tag="PK252_05720"
     CDS             40855..41820
                     /locus_tag="PK252_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14245.1"
                     /translation="MDLLTFINSNCKELLHLLNADEDGWQLPFIESINKAIHLNRWFD
                     KENILRRLRCTFEYYASDALINDLTANEKVIEQKTIGLISEERIPMEEITTILNLINA
                     GNKVIYKKPENYDIVLCSLFEYWQNKAGRELLPIEFTDRPIKGFDTLLITGREPWKGE
                     KRKFFEKHHVIEKTRYNSIGIITDSITNEQFKAMASDIFCYYGLGCGNIRKIYIPEGF
                     AIERIFEAIEPWHNVMLHNAYANNFQYHQSVFLMNKIKHLDNGFILLKEDAASYAPIG
                     ILHYQYYSDINKLKVLIDKENKYQILSNNPELGLKFGNADKQLLN"
     gene            41845..42753
                     /gene="miaA"
                     /locus_tag="PK252_05725"
     CDS             41845..42753
                     /gene="miaA"
                     /locus_tag="PK252_05725"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT14246.1"
                     /translation="MNKKFLIVIAGPTAVGKTAFAVTAAQYFSTEIISADSRQFYKEL
                     SIGVARPSEAELSLVKHHFIASHSIHDYFTAGMYELSVIELLNRLFQNHDVIVLTGGS
                     GLYIDAVCNGIDDLPDIDMDIRNKVLERYENEGIEPLVEDLKSIDPAYYQTADLANPK
                     RIFKALEIFYMTGKPYSEFLTNPKKERNFNIIKIVLNKAREELYQQINQRVDMMMETG
                     LLDEARNLYPLRKLNALNTVGYKELFEYFDNKITLKQAIDKIKQNTRHYAKRQITWFK
                     RNNDYTWFDATNKELILQFLQKEIHA"
     gene            complement(42750..43517)
                     /gene="mazG"
                     /locus_tag="PK252_05730"
     CDS             complement(42750..43517)
                     /gene="mazG"
                     /locus_tag="PK252_05730"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT14247.1"
                     /translation="MDPRLQAFQRLLDIMDELREKCPWDKIQTFDTIRNLTIEEVYEL
                     ADAIIEKNLPEIKKELGDLALHLVFYAKIASETGAFDITDVLNGICEKLVFRHPHVFG
                     EVSVSNDSEVKHNWEKLKLREGNRSVLGGVPHSLPALVKANRIQEKVRSVGFDWDNPE
                     QVWDKVKEELEEIQVEIKSGDKQKLEAEFGDLLFSVVNAARLYGIDPETALERTNRKF
                     KKRFEYLEKESSDMHKKLKDMTLDEMNAIWEKAKKFD"
     gene            complement(43521..44342)
                     /gene="panB"
                     /locus_tag="PK252_05735"
     CDS             complement(43521..44342)
                     /gene="panB"
                     /locus_tag="PK252_05735"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT14248.1"
                     /translation="MYISDQLNLKKITTHKLSEMKLRGEKISMLTAYDYSMARILDQA
                     GIDVILVGDSASNVMAGFETTLPITLDHMIYHAASVARAVNRALVVCDLPFGTYQGNS
                     KEALASAIRIMKETGAGAVKIEGGIEIQESVERILSAGIPVMGHLGLMPQSIHKYGTY
                     VVRAREEAEANKLVEDAHLLEKIGCFSIVLEKIPAVLAKKVAGELKIPIIGIGAGNGC
                     DGQVLVLHDMLGLTKEFSPRFLRRYQNLFDIITNAVQRYVSDVKTGDFPNENEQY"
     gene            44709..46613
                     /gene="dnaK"
                     /locus_tag="PK252_05740"
     CDS             44709..46613
                     /gene="dnaK"
                     /locus_tag="PK252_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT14249.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVITNSEGKRTTPSIVAFTG
                     NGERKVGDPAKRQAITNPKHTVYSIKRFMGETYDRVTKEIQREPYDIVKGDNNTPRVV
                     IEDRKYSPQEISAMILQKMKKTAEDYLGTEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LNVRRIINEPTAAALAYGLDKKHQDMKVAVFDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDQVIINWLAEEFIKDEGIDLRKDPMALQRLKEAAEKAKIELSSSTSTEIN
                     LPYIMPVNGIPKHLVKNLTRAQFEKLADSLIQACIEPCRKALSDAGLKASDINEVILV
                     GGSTRIPAVQKIVEDFFGRTPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIEANTTIPTRKSETFTTAADNQSSVEIHILQGERPMASGNKTIGR
                     FHLDGIPPSPRGVPQIEVSFDIDANGILHVSAKDKATGKSQSIRIEASTGLTDEEIKR
                     MRDEAKANEAADKQAKERIDKLNAADSLIFQTEKQLKEFGDKIPADKKGAIESALANL
                     KEAHKSQNIEAIDKATAELNHVWQAASEEMYKAQAQQGQAGGSSAGAQDNAKKSGDSE
                     VTDVDFEEVK"
     gene            complement(46755..47177)
                     /locus_tag="PK252_05745"
     CDS             complement(46755..47177)
                     /locus_tag="PK252_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14250.1"
                     /translation="MIANFDWKDKTILIAEDDYYSFMYLKEILHHSGIKMIHVENGLR
                     AFSECIKNNQIDIVLMDVKMPVINGLESTRLIKKYKPAIKIIAQTAFAMYDDMSKCIN
                     AGCDDYITKPIKPEELILKLEKHFSGKASEQVEVKFLN"
     gene            complement(47262..48287)
                     /locus_tag="PK252_05750"
     CDS             complement(47262..48287)
                     /locus_tag="PK252_05750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT14251.1"
                     /translation="MYKASVVILNWNGIGFLRQYLPLLVEHTTFKDVELVVADNNSSD
                     GSQEYVKQMFPTIRLVEFSKNYGFAGGYNLALSKIEAKYFILLNSDVEVTKGWIEPLL
                     DFLDKNENVAAVMPKVLSVHDRSMFEYAGAAGGYIDIFGYPFCRGRVFDHLEKDNCQY
                     DDVAEVFWATGACLTIRSEVYKAVGGLDEHFFAHMEEIDLCWRVHWAGFRIFCVPQSL
                     IFHYGGGALPKENPKKIFFNIRNSLYMMLKNLPLAYLPFILFCRLFFDAVAGFQFLLK
                     KKPAFAWAVVRAWFSFFVKVPFYLNYRQKSHKTRVSKEWKKLVFHKSVVCSYFVLKKQ
                     TFSQIIK"
     gene            complement(48274..49167)
                     /locus_tag="PK252_05755"
     CDS             complement(48274..49167)
                     /locus_tag="PK252_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT14252.1"
                     /translation="MALFIYCIVFPLLWLISLMPLRLLYVFSDIVLYPLIYYVANYRK
                     KVVYENLRKSFPEKSEAEIAFIARKFYKHFGHILVETIKLIHISHAEVKKRIRYRNPE
                     VFFELFEKGKSIVAVTAHYGNWEWLVGTPENMPYRIISLYKPLSNKYIEKIMTRMRTQ
                     FGAELVTMEEAPRVLFTYKQNNVKTLSCFISDQTPIRSRIHYQTQFLNQVTPVFLGPE
                     KMARKLNSAVVFVKMIPVKTGYYEFEIVPITEDASLTNEYEITNKHVKILEEQIKEHP
                     EFWLWTHRRWKYQILDRDVQG"
     gene            complement(49167..49961)
                     /locus_tag="PK252_05760"
     CDS             complement(49167..49961)
                     /locus_tag="PK252_05760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT14253.1"
                     /translation="MQLEKICLNVCQLARETGLFINQEGEKIKAENVEVKGLNNFVTY
                     VDKNAEAKIVEGLKKLLPEAGFIAEEGSGKEAKGALNWIIDPLDGTTNFIHGMPPHAV
                     SIGLADGEELLAGVVYEVKADECFYAWKGGNAFMNNKQIHVSKAPTVADSLIATGFPY
                     YDFDQMQAFMGTLEYLFQNSHGVRRIGSAATDLAYVACGRFDSFYEYNLNPWDVAAGA
                     LIVKEAGGQVSDFKGESNYLFGREIIASNNNTHKEFLALINKYFYK"
     gene            complement(50009..51298)
                     /gene="mtaB"
                     /locus_tag="PK252_05765"
     CDS             complement(50009..51298)
                     /gene="mtaB"
                     /locus_tag="PK252_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT14254.1"
                     /translation="MMRKVAFYTLGCKLNYAESSMLQRKFEQSGYQKVSFDAEADVFV
                     INTCSVTQVADKKCRQAIKKATSKGAKVVVIGCYSQLKPEEIAAIEGVDLVLGTADKF
                     KLVEHVESLWNGNAVKIHSCDIASVNSYEASYSISDRTRAFLKVQDGCDYHCSYCTIP
                     LARGSSRNEDISSVVSRAKEIAAKNTKEIVLTGVNIGDFGKTTGENFFELVKQLDKVE
                     GIERYRISSIEPNLIHDEIIDFVKSSNRFAHHFHIPLQSGCDKILAAMGRRYKREVFA
                     ARVKAIKEVMPYACIGADVIVGFPGETDDDFMDAFEFIKSLPVSYLHVFPFSERPNTK
                     AFDMKGKLSPQVKERRSKALIELGNVKKEAFIQENIGRKETVIFESKLKDGQMHGFTS
                     NYIKVSAPYDKKLIAALSAVTLDEVLDNMLVKASIVS"
BASE COUNT        17514 a        10323 c         8560 g        15126 t
ORIGIN      
        1 gttcaaccgg ggcttcatgt tgggctttac aagccacatg aagccctatt tattagttta
       61 ggttataatt tccatttttc aatcactaaa caacaacaat gtaccctact tgcataattt
      121 tttctatcat ttattgaact gtaaactatc gttaggtgta acctgaggct cttctatttg
      181 aatttcaggg gtttcgttaa tatcatcctg gttttgaagc tgtaaatctt cttctgttgt
      241 tccgtttttc catcttctaa ccaccctaac aactttgtct tgtggagaaa gaacaaatga
      301 atatggtgtt tgaaaacgtc caacagttat acttctttct ttcattgaca tttcgcgaat
      361 aggaagctca aattgcttat ctgaagaagc aacccacatt gccccgcgtg tataaccaaa
      421 ataaaaataa tttttctcat ccacctgcaa ataaatatcc atagcatcgc ccgaacgctt
      481 acgccaaatc tcaacatatc cgttgacata acgatgcagg ggtttgcccc caataaagcc
      541 aatgccaatt ttcccttcag aacggaacga cttcgtagcc tggtcccata ccaggtttat
      601 atcatctata aatattgtac ttttaaaggc ttctggcaaa atattttctt tttctttctc
      661 agacaacatt ggcaggtcaa actctgtaaa aaatttacga atggtagcag tatcagcaaa
      721 ttcgccatat aattttctga aatatggtgt ttttattttg attgaatcac ggcacaagct
      781 gtcaatgtac ttcctggtga tttccaacga aggttgataa aaatgaaact ttacagccat
      841 tgccatgtgc agttttactt tatttgcaac caaatcatgg tcaatggttc cggcattaag
      901 cagttttact tgtccaaggt ttacgcctgt ctttaatttt ccttcgccat aaaaacgaca
      961 ggttttacgg ttaaaagtaa taagctgtcc gggcaaactt ttgtttttca acttttcttc
     1021 gctggcaact tcataattgc caagcttttt gttaaatatc aacaatcctt tagacttaat
     1081 caaacttgta tcactatgaa acttgcgtgt accaaacata gttgaatata gcataattgt
     1141 atctgcccgc aaatatgaac ccaatacaat ttttttatca ttgatttctt ttaaggtgtc
     1201 acccacagga attttaacgt cagtggggtc tataacagtt ttaaaatcaa accaactggt
     1261 tccggtatta gggcattcaa ccacaatttt tgtagagccg ttaaaggtaa ggtatttttc
     1321 tttagcaaat atttgggctc gtccctgata cataaaatcg gggctcagca taaaactatc
     1381 aggttctaca atagtaccat cagtaaaagt agaattggca gagtccacag taatggtatg
     1441 cattgaaatc acctctggta tattggttac aggaatatag tcgtattttc ccgaagcttt
     1501 atacatcttg cggcttgcaa tatcaacagt agcatcgtaa atattatgga aacgagtttc
     1561 ccggttagcc aaaattttcg atttataaag aagttgtact tttgagatac tatcaataac
     1621 aatcttttct tggtttggaa aaatacgagc atctgcaata tcaagatatt tgacctttga
     1681 agcttctata ctctttgttt tattaatata aacagcactt cccgagacaa accaaagcga
     1741 atcctgcagc ggggcagttg acacaaatct tgctccaatt aaaggctcat ctttaaagcc
     1801 atataatttt ccttcctgag gtacaacatt ttttcgagca gaagacatta ccaattcatc
     1861 tttattcatg taccatttca acaaatcaat cttggctata tatctatttt cggggaattc
     1921 ggttattaca gcggtatcat ttgttttaaa atcgcctata cgtgtagcaa agtttatatc
     1981 tgacttaaca ttgttggttt ttacggttat ctgcctgtct aaagatgatt tcaggcgaaa
     2041 acaagaagta tcagcatgtg ccaacatttg ataaaacgtg aaattttttg acgaattgtc
     2101 agcggtaggg aaatttatca ttccatttcc tgacaatttt ttaggggtca atgataataa
     2161 cccgttaaaa ttggcttgtt tcgagtacat atcaaactga ttggccgtat ttgatgcata
     2221 catcacatct ttgtatggct gccaattaac ttttatggct tttccttcaa cattaggaaa
     2281 tgatgcaggt gcagcctggg cttctatcgt aaaatcatct acagcatcca tgctgtcggg
     2341 aaaaaatatt atatcttcag aagtaacagt agctgaaagg tatttcacat ggcctttccc
     2401 gcgcagaccc tgattagaca aatttaacaa cctggaaaac ttcccttttc caccataagc
     2461 agggaaaccg gcatctggta catcatgggt aaaacctaaa gaaaaatcag gctgtagtac
     2521 caacttctca ttaatattag gaaatatttt agccgaagaa agaaaaccat tgaatttcat
     2581 cccttctttt gaaaagttat ctaaactatc gagcacgtaa ggctcgattc tgaaataaaa
     2641 atcacgcgaa cgtttataca ccccgccaaa aatagaagga tgatcgtagt aaacatacga
     2701 attgccacga ctggtaaaaa tgggatattc aggatagttc accacccccg atttattgtc
     2761 aggggcatca ataagtatat cgcctgttac atcttgtata acgttgttaa catcctgata
     2821 aatgggcttc atagcaaggt cataaccaac aataactttc atcctgatag agtcaacatt
     2881 tcctaatgaa accttaaaac tatcatagtt aaaaaagaaa ttgcgtccaa aaaaagtgaa
     2941 caaacctgct tcaacaatac catcaaattg aaaattgcga ttatgcttca actttatggt
     3001 ccgggctttg ggatagattt taacattttg gctatcacta acaaaaactg caggtatgcc
     3061 atgtattgtc aggtcaaaat tgcgtaaatt aagcgaagca ttttcaagag gagcagaagt
     3121 gttagaaata aagcgcagca cgtcatagtc ttttcttcca atgcttgact gtaaggcatc
     3181 aaaaagtctg tccttcagct caaccttttc tgtggaaggg tcataataaa tatatccttt
     3241 cacagccaac ggaaaaagca acgtcctaac ctgatccatg ggccgtttga taaaattggc
     3301 aaagtcaatg gcggtgaaat tgcggtcacc attgtaatat tgcgaacatt ttttgagctg
     3361 cgaaagaggg tgcttgtcat cataatactg taatgcttca aactcctcat atttaaaata
     3421 atttacagac tcaaaaatag ctttaccagc agaagagcca agtgcagaag taagtaatat
     3481 aaatggctca tcgatatgcc agaccattcg gtcaaaattc atatctatat tatgataagt
     3541 atttaaaaaa gggctttggg ccacataatt gtccgatttc tgcagagtaa gttctttatt
     3601 tttagaaaca taactaaact gtacatctcc atgataaatt gaatcttttt cgagaaaaat
     3661 agtgaccata gcattgcttg ctgtcacctt atgcggttta aaaccaaaag taaccgagcc
     3721 tgcaacaagt tttaacgtgt catgaaggaa aaaataaagc tttgcaggtt catataccgt
     3781 gccactacca ataagtttgg ccccttgcat gcttaccccg cctttataat tcacatcagg
     3841 gtagatattt ttaatgttat attctttaat atacgaatca aattgcggat attgggcatt
     3901 ctcaggttta gaaatataca taacattgtc ttccaacttc ccgagcatgg gggcagaaaa
     3961 gaaactttta ttagtaaaac tcaccgaatc tgcctggtat gaagttcggc taaggtctat
     4021 tgtataacct gaaagaattg ccgaaaccga atcaactgaa tagcctgctc tttcccaagt
     4081 aacgaggccg ccttttccat accatttatt ttcaaaaggg tcatagctcc ctgttgtgtt
     4141 ttttatcatg atgctatcac gcttagcacg acaagttaag tctgtttttg gaaaaaagaa
     4201 aataagcctt tcttcatttt taaccacaaa atctggcgaa gatggcaacc atgacaagga
     4261 aggagcttta taaagtaccc gttgtttaaa aaaaactata gtattggcaa gcacattgcg
     4321 caaagctgtt gctgaaacct tttttgattc gaccttttct gccatagctt caagccatgc
     4381 cttactatgc cggggatatt tgctattcaa aataataaca acgttgatat accccataaa
     4441 atcaggaaaa ggcctgaact ttttatctat caggccaatg gccgtcctga ggataaactt
     4501 tttgttttct gaagatattt tggccgaatc ccataaagcc gtgaaaacgc caagttgcac
     4561 taaagccgaa tcgtcttcta cttttgccat aaacaaccgt aattcattgg taaacaatgt
     4621 atctttacca gagaattgtt taaacgactg ttcgctggct ttggctgtaa aacagcaaat
     4681 cagagatact acagatagta agcaaactaa tgtttttcgc acaacagaca tgcccatttg
     4741 ttattggtag tagtttttaa aatagataaa ttcagtttat caaatatagc tttaagaaaa
     4801 ataatatctt cttcatagaa accactaaaa ataatatatc cacctttttt taaatatttc
     4861 atccattccc caacattatc tgcaatggtg tttctgttga tattagccag aatgatatca
     4921 aacattttgg aaggaattgc tcctgtgtcg ccagtaatag caataatttc tgtacaacta
     4981 tttcgctcaa agttttcaat ggcattctct accgcctgag ggtcattgtc aataccaaca
     5041 atatcaacag ctccaagctt gtgtgctaaa atagccaata ttccagttcc gcagcccata
     5101 tccaataccg tagcaccttt catttcaact tcaagcatca tcagcattac ctgcgaagtt
     5161 gtttcgtgat gaccggtgcc aaaagacatt tttggttcta tctttattac atactttgta
     5221 tcggggttga tttgatgaaa cggagccacc acagcaatgc gggtatcgat aattacaggc
     5281 tcaaaattgc tttcccaaac ggcattccag ttctcatcct taattttttt aaaggtatat
     5341 ctcacttccg aacgtgtaat caactttgaa agtgcttttt caaaaaagct taccttatgc
     5401 tcatcttctt ttatataagc ctgaaaacct gtttcgtttt cgacaaagct ttcaaatccg
     5461 taatcaccaa gcagggcaat taaagcttcg ctggtttcag gctgaaaagg ttcaattgta
     5521 acattatatt ccaaataccc cataaacact acattttacg ataaaataaa aatatcgtaa
     5581 atctacacgt tttttttctt tgacataaac tcatacgaaa ataaaattta cctttgcagt
     5641 ccaaaaaaaa tgtactaatg gataagaata gtgtaattgg gattataatc attggagcca
     5701 tacttatagt tttttcggta tggaacacac ctaacaaaaa ggaacttgaa gaagccaggc
     5761 gaaaagccga ctcaatagct ttagtccaaa aactggaagc tcaacaaaaa caactggaag
     5821 ctaaaaaaga cactgttgct gcaaaagctt tagatgcaaa atctgaagct caaattgcta
     5881 ctgaaaaata tgctgaattt ggtcaagctg ccacaggaga ggctgagttt tacactatag
     5941 aaaatgaatt ggtaaagctt accctcagta aaaaaggggg aaaaccttat tcggtagaac
     6001 tcaaacagta taaaactttt gataaaaagc ctttaatctt gtttgatgga gattcaaacc
     6061 agtttgggtt caacttttac accgccaata acaggcttat ttcaaccaat gaattgtatt
     6121 tcacacctgt tcaaacaaat aaaaatgttg ttttggcaga aaaagatact gctgcaagta
     6181 tttcatttcg cttaaatgca ggtgaaaaca aatatataca atacacctac acactgcatc
     6241 ctgcaaacta taagattgac ttcaaagtaa aatttgtaaa cctcgatagt atcatatctt
     6301 ccaatactac ttcaatcgac ctgaaatggc atgttttcct gaaagctcag gaaaaagagg
     6361 tccctaacga aaaaacatat actgaggttt tttataagtt caaccaggaa gatgttgaaa
     6421 gctttaagcc ttctaaagaa aatgaaaaaa ccgagaccct ccgtaataaa atcaaatggg
     6481 tagcattcaa acaacaattc ttctccagcg tgcttattgc tgataataat tttgaaaatg
     6541 ccaaccttac tataaaaaac aatacagaca cgtccaggat attaagcctg atgaatgctg
     6601 agcttggcat tgcctatcaa ccaggaaaga ctaatgaagt tggctttgct ttctattatg
     6661 gcccaaacca ttaccaaacc ttgaaaaaat atgatcttga cctggaagag cttgttacac
     6721 ttggttcgtg gattgttaag tggatcaacc gttttcttat tatcccggtt ttcaacttcc
     6781 tgaaccaatt tataggaaac tatggtatta tcatcttgtt gcttaccata tttatcaaac
     6841 tgttactctt ccctttgaca tacaagtcat acctttcaat ggcaaagatg agggtattga
     6901 aaccgcagat tgacgagata aacgaaaaaa tacctgccga caaacctatg gaacgtcaac
     6961 aagccacaat gaacctttac cgaaaagtag gcgtcaaccc gttgggagga tgtttgccaa
     7021 tgctgttgca gttcccgata cttattgcca tgttcaggtt tttcccgtct tctatagaat
     7081 tgcgccagca aggcttcttg tgggccagcg acctttcatc ttatgatgct atcataacct
     7141 ggtctggaca tattccgctt attactgaat attttgggaa ccacatcagc ttgttcaaca
     7201 ttttgatgac cattacaaca cttattacta ttaaaatgaa cgaccaggcc aatgcccaac
     7261 agcaaatgcc cggaatgaaa gtgatgatgt acataatgcc tgttatcttt atgtttgtac
     7321 tgaaccgctt ctcagctggt ttgacatact actacttcct tgccaacctt atcactattg
     7381 cccaaaacga aatcttcaga cgttcaattg acgaagctaa gcttttgcac cagttgaacg
     7441 agaacaagaa aaaaccgatg aaaaaatcaa gcttccaggc tcgtcttgaa gaagcagcca
     7501 ggaaaagagg ttataatact aaaaaataat gcccgtggga cgattgttcc tgataccaac
     7561 aacgcttggc tcagacaatc cccttgaggt tttgcctgca tacaacgtag aagtgctgaa
     7621 aaacatcagc acttttattg ttgaagatga gaagactgcg cggcgtttca tcaaaaaaat
     7681 atggcgcgaa gccatcattg acaatatgca gtttctactg cttaacgaac atacaaaacc
     7741 cgaagatact gttcattttc ttgataaatg tacccacgaa gacattggcc tgatgtccga
     7801 agcgggtgtc ccctgtattg ccgaccctgg caatattata gtaaacatgg ctcatgctaa
     7861 aggcatagag gttgtaccat tggttgggcc ttcttctatt atcatggcgc tgatggcctc
     7921 cgggcttaat ggccagaact ttgctttcaa cggatatttg cctatagaaa aacctgcaag
     7981 ggcaaaagcc ttaaaacatc tcgaaaaccg ctctgcttct gaaaaccaaa ctcaatgctt
     8041 tatagaagcc ccatatcgca acaaccagct cgttgctgat atgctacaat gcttatctcc
     8101 tgctacaaag ctttgtatag cctgcgacat ctcccttcct tcacagttta tcaaaatgca
     8161 aaccattgac cagtggcgca aaaaagtgcc cgacctgcac aaacggccag caatattctt
     8221 aattagcaaa tgaaaaaaac aatttgaaaa tgagagaatt tgaaaattat agcaagtttc
     8281 ctgccaaatc agcgagatag cttctttctc ctttcaaaag atgaatgtgg gcaaatattt
     8341 cctgcccttg cattttgttt gtcaagtaac ttaagccgtt tgaaaagcta tcgaggtacg
     8401 gagagtctat ttgctggata tctccggtaa acaccatctt agtcccttcg ccagcacggg
     8461 taatgatagt ctttacctca tgcggggtca aattttgagc ctcatctaca ataaagaaag
     8521 cattggacag gctacgccca cggatatatg ccaaaggtgt aatctgaagc ttctcatttt
     8581 tctgcatttc gtcaatcttg gcagcttctt tgctttgaac tttcagcttg tgcttgataa
     8641 acgaaaggtt gtcgtacaaa ggttgcatgt atgggccaat tttctcgttt acatcgccag
     8701 gcaggaagcc aaggtcgcga ttggcaagcg gaacaatagg acgagtaagg taaattgtct
     8761 caaagtcttt ttcttgctgc aacgcagcag caagagcaag caaagttttt cccgtacctg
     8821 ccttcccggt aagtgccaca agctgaatct caggatccat caaggcattg agggaaaagg
     8881 tttgttcggc attgcgaggc tcaatgccat aacagcgaag cttttcaact ttttcaagat
     8941 tcgatgtacg cctgttgtat cgggctaaaa cgctcgtttt ttcattgcga ataatgaaat
     9001 atttggcact ttcgtctgta accttcttct tggtgacgtt tgacaatgga atgccatctt
     9061 gcaggttgta caactcagat atatgcttag ggtcaacctt ctcaatggtc tcaatgctat
     9121 ggaaaaaagt ctccagatcg tgcactttgt cagatttgta gtcctgagcc aggatgccaa
     9181 gcgatttggc tttcactctc aggttaatgt ctttggtaac cagcaccgta gtagaatatt
     9241 gccctttgtt actctcctgt atatattctg caatagccaa tatacgatgg tctggcgtac
     9301 tttcaggaaa agaagcctgc aaattttttg aaaagggctt cccggtaata ataaaaagtt
     9361 tacctaattt tttacccaac ggaataccag aagtacaaag cccttcccca gataaggcat
     9421 ctaactccct tgtaaattcg cgggcatgca tgttcaatat atcattgcca cgtttcaact
     9481 tatcgagttc ttcaagcaca acaatgggaa cgacaatgtc attttcttca aaattgtaaa
     9541 tacatttgta gtcatgtaaa atcacgtttg tatccagtac gaatatcttt ttttcttttg
     9601 ccataatttt attattaact cattaaagat aggaaaacct ttgaaatgat tcacacatat
     9661 tctgaagcaa tcgaatattt atattcgcaa atgcctgttt ttcaccgcga tggagccagt
     9721 gcttacaagc caggcctgga aagaataaca aagctatgta ctattcttag actggataag
     9781 cttgacttca aaacaatcca tattgccggg accaacggta aaggctctgt tagtcatgga
     9841 ttagcttcgg tattgcaaac ttcgggatat aaaaccgggt tgtttacttc gcctcatttg
     9901 gttgatttta ctgaacgtgt taaagttaat ggcgaaaaca ttgaaaaaga taaggtaata
     9961 caatttatca accattcatt tgaggctata caggaagtac aaccatcttt ttttgagcta
    10021 acaacagcct tagcattatg gtactttaaa ataatgcagg ttgaaattgc ggttatagaa
    10081 accggacttg gtggaaagct tgattctaca aacattattt cccctatctt atctgtaatc
    10141 accaacatat catacgacca tatagatttg cttggcgata cccttgaaaa gattgctatt
    10201 gaaaaggcag gcatcataaa accaaatgtt cctttggttg ttggccaaac acaggctgaa
    10261 actggaccaa ttttcatcga cacagcccaa aagcttaact cacctgtata ttttgcagat
    10321 aaattgctca aaatgacaaa tgctaaggtc actaacgaat ttaaacagaa aatatcagta
    10381 atagccaatg atttgactat gaaagaacat gaaggcgatt atttaattga ccttcaagga
    10441 gtatatcagc aaaaaaatat attaaccatg cttacagcaa tagaccagct taaaaagcag
    10501 aatattacta tcacacatga gcaaattaaa ctcgggttgt catctattgt tgaaaaaaca
    10561 gggcttttag gccgttggca ggttataaac caacatcctt acactgtttt agatacagga
    10621 cacaacgaag acggtatcag gtgtgttgta gaacaactta acactatacc tcacaacaaa
    10681 attcacatca tctttggaat ggttgcagat aaagacatca acaaggtgct ggcgttgctt
    10741 cccaaaaatg caacctacta ttttacaaaa gctcctgggc caagggcttt aaatgaacat
    10801 cttttgcaac aacaagctaa acaatataat ctgcaaggta acacttttga aacagtcgaa
    10861 aatgcttata aagatgcctc tttcaatgca caacccaacg atattattta cattggtgga
    10921 agtacatatc tggtaggaag atttttagag attagaaaga atatggaaga gtaaggaaga
    10981 aaaaacgcag tttctactta aactttttag agcttaaaac attagctata tatcagtaac
    11041 gacttcctca ctcttcccta aacttctatt atcttcctta aacttccata cacttcagca
    11101 atcttcaata ttcttctata tcaaaaactg aaacaaatcg ggcttttcat tgaggtattc
    11161 ctgcaaaaag ttgcttgctt tgagacgttg aaatagtggt tcaaaatctt ctttcttttg
    11221 tagttcaata ccaacaacag cagggcctaa ttcacggtta ttcttcttcg agtattgaaa
    11281 atatgaaata tcatcattag ggcctaaaat attttcaaga aattcgcgga aagcaccagg
    11341 tcgttgagga aatttcacaa taaaataatg ctttaaacct tcgtataaca gcgaacgctc
    11401 ctttatttct tcagttcggg tgatatcatt gttgctaccg ctgacaatgc atactacatt
    11461 tttgcctcta atctgctccc tgtaaaaatc gagtgcagca atagacaatg ccccggcagg
    11521 ctcaacaact atagcttctt cattatacag ttgcataatc acgctacata ccttaccttc
    11581 cggaacaagg ataatgtcat ctataatatt tcggcatatt tcaaaagtca aatcccctac
    11641 cctttgcact gctgctccat ctacaaactt gtcgatatgc tgaagcgtta ccacttctcc
    11701 tttttcaatt gattctttca tagatgccgc accagcaggc tcaacaccaa ttatttttgt
    11761 atcggggctt aacgtcttaa atacgctccc cacaccggct gcaaggcctc ctccgccaat
    11821 aggcacaaac aagtaatcaa tgtgcgaaga ggtatcagac aatatatcta accctgcagt
    11881 agcttgtcct tcaataacat gaatatcatc aaaaggatga ataaattcaa gcttttgctc
    11941 atttttgaac tttaaagcag cattgaaggc atcatcataa gtatcgccag ccaaaataat
    12001 attaacatat tcgcgtccaa aaaattcaac ctgcttaatt ttctgctttg gagtaggcgt
    12061 tggcataaat atatgtcctt tgatttgcaa ttttcggcac gaataggcaa ctccctgggc
    12121 atggttcccg gcactggcac aaacaatacc ttttttgagt atatcaagtg gcaacatact
    12181 gattttattg aatgccccgc gaattttata tgaacggaca acctgtaaat cctcacgttt
    12241 taacaatata ttacaatcaa aacgctcaga aagattgaca tcatgcatca aaggagtgtt
    12301 agtaaccacg ttacgaagac gttcacgggc tttttgaacc tggttcaatt ttattttaac
    12361 cgatgattga gataaagcag acattctata agtgtttttt atttttaaag tgttgagaat
    12421 caccccattg gccataccca actttattgc cggtaagaga aatccgaaca tcaagacaag
    12481 acaggcagtc atctgcagcc tcatcggtgc atggtttatt ctcatacaac ttataatcag
    12541 tgcggtattt ggcaggagta atattaggca taataatatt ggcgccacac ttcagggctt
    12601 tttcacgacc caaagggtct atagcctgaa gtgctgttgt agctgcaatg ttaatatctt
    12661 tcataagcaa acgtaatgta gccaccattt tcagggctaa gtcaaagcgt tgtttttgac
    12721 tatcgagcat ttctcgcttc tcatacatag gggtatcttc atgttcgagg tatggcccca
    12781 ttccacacat atcaatatcc agttccttca taaacaaaag gtcttcaaca aggttgtcta
    12841 tagtttggcc tggtaagccg atcattacac ctgtacccac ctggtaaccc aactttttga
    12901 gcagcttcaa acaatctaag cgcctgtcaa aattgtgcat tgcatcatta gggtgcattt
    12961 gtgcatacaa ctgcctgttt gagctttcta tacggagcaa ataacgatga gccccggcat
    13021 ttttccacct ttggtatgtc tcttcgcttt gttcgcccat tgataaagta actccaagct
    13081 gtccatttgt taaaagcctg atttgccgga gaaggttctc aatacgttca acaaaagccg
    13141 gcgaagtcat ttccccggat tgcaaaacaa tagacccata gcggttttta aaagcaaata
    13201 atgcggcatc tatcacttcc ggatcagaaa cagtatatct tttaattttt ttgttgcttt
    13261 tgcggatgcc acagtaaaaa caatcttttt gacaaacgtt ggaatactct attattcccc
    13321 taaaaaaaac tttattacca ataatagatt gctttatttc attggccttt tcaaaaagcg
    13381 ccttcttttg ctctccttca gaatttaata acacctttaa atcagatgct tcaaagcttt
    13441 caagtgctaa tattgaagaa aaaatttttt gcatataacc ttttaaagca ataaaacatc
    13501 aaagctgtca aatagtttga aaatcgaaaa ctaaaatgct gtttttgctt tggccccaat
    13561 ctacctgtaa aacatcgaaa acataatatg ttgtgggata caatataaaa gtaaaaccac
    13621 taatgggatg ccaataatca aaaaacgtgc cttaggtttt tgacgcattt taaaaacgaa
    13681 atataaaaac aaaaaggcca caagatcttt gtaaaaagta aggttattat gagggtcaaa
    13741 tgccaaaaaa atatgcctaa atgaaatcag gtgtataaca aaactaaaca aaataacacc
    13801 tacagagata taaaaactgg cccgaagata ttttgcatag gtcctgatgt tataaatagc
    13861 taaaatacac acatatatta ataaaagcac tatcaacaaa tacaaaaagt cataaggata
    13921 agagatgtac tttggtactg tactctgaaa ccttaaccag accgggttat ctttagctac
    13981 agctattgct gtaccacctt ttattagctc cacataataa ataaccttca catttggctt
    14041 aaatggaggt atcattgtaa ctaatgtatt tccggtacgt accattggtt ggactttcca
    14101 tgccatcgaa gaattggcca accgataata tatttttccg gtgactgaag tatcaggtat
    14161 ttcaaaatcg acaaggcatt catccttgcc ttcagaaaca atcggaaatt taagcttata
    14221 ttctttttcg ccaacaaaag cataatgaac aacaggcctg tccgaatcgg aaaattgtct
    14281 gataaaaacc gcacaaatag caaccccaca agctatcaac aagtatataa cttttttcaa
    14341 catcccttat caacatgatt aatgaatttc aaatatacga ttttatagtg aaataaaact
    14401 gaatgtacca cttaaacaaa tgacttcttc ttccatctcc cggtacgata ataaaaatag
    14461 ctaaaaatag ccccaataaa ccatgcaata ggaattgacc accagatacc tttttctgca
    14521 tacttccctg aaagaaaaga agctaaagga agccttacaa accataatga gaaaagggtc
    14581 accagcatag ggataaaggt atcgcctgcc cccctcatta ccccgccaaa aataaacatg
    14641 gcagtaaaca cagcataaaa agaacctgct atccgaaggt actcatgccc atacctgata
    14701 acattagcat cggtcgtaaa catattgata agcaaatcgc cagcaaaaaa taccaatacc
    14761 gaaatacaaa gtgcaacaca tacagacatt ttgagtgttg caagcaaacc ggtttttacc
    14821 ctttccatct ttcctgcccc aatattttgg ccaacaaaag cagaaagcgc catagcaaaa
    14881 ttcatggctg gcatagaagc aaacatttct atacgatacc ccaccgaata agccgctata
    14941 acatctgtcc catattggtt aacaattacc atcagagcca tcatagcaag cgctacgaag
    15001 gtattttgta ttccggtagg taaccctatt ctcattattt ttgaaaaaat aagcctgtcc
    15061 cactctatgt ttcgaataga aaacttcagt agtttgtgag ttttattgag ataaataatg
    15121 gccgcaataa atgctaccga ctgactaatg atagtagcaa cagcagtact agccactccc
    15181 catttgaaaa taccgacaaa aagcaaatca agcaacgtat tgattatcgt actaataatc
    15241 aaaaaataaa gcggagtttt tgaatcgccc atgccgcgaa gtatagcgct aatagcatta
    15301 aacccaaaga aaataaacgt acctgaaaga tatacattta aaaagcttgt agctataggt
    15361 attaaaggct caggaagatg catcatcctg aatatttggg cgctaaatat tatacctaat
    15421 gtggataaaa ccattgacaa gaaaaacaac aaaacattaa tggtatctac agtcctttta
    15481 agcctttgaa aatctttagc gccaaaaaac tgggctacaa gaacagtgcc ccccatggaa
    15541 acaccaatag ccaaagaaac aataacaaat atgatgggaa acgacgcccc tatggcagca
    15601 agggcttctt taccaataaa atgcccaact acaacactat ctattatttg ataaagttgt
    15661 tgaaaaacat taccaataat cattggaact gaaaattgcc agattaactt cccttcatgg
    15721 ccaactgtaa ggtctttcac aaaaataatt tttgcaaata tctaaatata ttctcattta
    15781 gaataaataa atattagcaa tatctgtatc tcacctcaaa atctttctga caaaagccac
    15841 agtaaagtaa ctcatcataa acacccccaa gaatccttct aaagcagaga ataaacgact
    15901 tacccccata ggaaaaacgt caccataacc aatagtaaaa aaggtaatag cgctatgata
    15961 aaaagattgg gcaaagacag agatattatc cgggttccct acgccagaca tggttttccc
    16021 aacacccaac aactctataa aaaagtagcc aaacccaaat aaaaaccaac acacaacaac
    16081 actaatcaac actcttgatg gcgatgttgc atacaagccc atgtaatcaa aaactatctt
    16141 tttaaaccaa tatacaggat aataaaaaaa tgactttaga gtactttttt gttgcatttc
    16201 atcaagcaag gctgccattt catgacgcct aaactctaca taagctttat cttcatcagt
    16261 gtattgcccg gtcacattga aattctgctt taacacccta aactgctcac atttgctcct
    16321 attactcaga ttgggctggt tatttatcat tgattttacc ctgtttttat cccaactaag
    16381 gtacatacgc cctacaagcc gcattccttc aaaattcaca cttttaatat caacagtcca
    16441 atcgtaaggc attaaatcta caatatcccg aacaatagaa tctgaaagat ctaaaaaatc
    16501 tgccttttta acacgtaaat caatataaca atcaaaatgg cacgattgaa gaaacaacga
    16561 tttgaacgct gaattatgaa agcttgttgt gcacatacca atatcagaag aactaaaaac
    16621 agcctcagta tcgggcatct caaccaaaga aaaattagaa gaaccgcctt gtattagcac
    16681 ttttttaaaa ttaaaacgtc ctgtcttttg ttcacaacct tcaaagttca ggtttacttt
    16741 gcccatctcg gccctgttaa aagacacctt ctggctccca aattctactt tnnnnnnnnn
    16801 naaaatcaat aaggccatta ccaaagcgac aacgttcaaa gcttacatcg cttccaaaaa
    16861 atctggcgta atgaaaatcc tttgccccat caccaaaagt agctactttg aaggaaacat
    16921 cacctgttcc aaactgagca ttgataaaaa aaacatctcc atgcccaaaa tcagtgttga
    16981 caaaattaat attagctttt tcaacatcta tatatcgaaa atcttttacc ccatcaataa
    17041 atacagcatt tttaaaagac aaactattgc ctttgaaaaa agaataagaa aaatctgttc
    17101 gctgtacctt aaaatgacaa taagaaaaag atacattctc ggtacgaaaa tgagcattag
    17161 caaagtttat ctttggcgca acaaataccg tgttgtcaaa gcaagcctca gcgccttcaa
    17221 aaacagcata agtaaaatca atatcggatg aagaaaccaa aaaactgttt gtaaccgtaa
    17281 aatcatgtaa tacaattccc ttttttatat caagcttttc atttatacgg tattgatgca
    17341 aatcaaaatc atctataaaa acagaatcaa gctttatagg cttctcttct tttataagtc
    17401 tatataattc aatagcacta atgtacccca gcctgatttc tttacgtgca ttatggtgtt
    17461 tttcatttga acgcaacaag gccgtttgtc tgtacccatt cccatccgaa gtggtataag
    17521 gctcatcaat aatagataat tggaagtctt gcattttagt tcaagataag ttgattttct
    17581 tctgttttgt caaaaaaatt aataataacg ggcgttttgt ttgacaggtt aaacatttcg
    17641 gcaaggttgc ccctgtatag tgctaattca agccatccgg atgagttaaa caacgctaaa
    17701 agttctccat ctttagcctc ataatatcct ttactaattt tattcagggt atatcgtttg
    17761 ttcaatacat acaactcaaa aggcctgttt ttaccaaccc ttgtaaaatc ctccaaagat
    17821 atgtttgtga tagcattacc ataagaatca atataaaccg ggttaccttc catcgaattg
    17881 ctatcaattc tgggcaattg cagatgagct tctaaggtaa tatcccattt agtaccaaga
    17941 ttttcaacat tacctccctg ggcaagatat acagcaacat ctgcaaatac ttcataatca
    18001 gcaaaaacag tatttaaatc atgttcaagc acccacactt ctctggcagg ggcaataaag
    18061 atgtgtttca taacatcatt gtatgcccca ataaaataat gattgtcatg cttaacaaca
    18121 atatgcgggc tttgaggctt cttttcacta tcaacacaaa taatatgaac tgtacctttg
    18181 gggaaagtcc tgtaagtatt gcgcataata tatgcagccc tgacataatc aaaagatgat
    18241 ataccatgtg aaatatcaac aagctgtaca ccgggcaaat tggaaataat cttacctttc
    18301 atggcactca aataaaaatc tccacggttc cagtcagata tgattgtaat tacaggcatt
    18361 tacaaaaaat tgaaaataaa tgaaattcag aaaaaaataa cgagcaaaag agttttaacc
    18421 tacatttgta ttaaaattca aaaataaaaa atatagctgg attcgatgac agaaaaagaa
    18481 atttacattg aagaaataga cccgggcata ctttttggag tccacaatgc caatatgact
    18541 ctcatccgtg aggcttttcc tatgttgaaa attatgaccc gcggtaatgt cataaaaata
    18601 ttgggcgaca agtacaatat tgaagctttc gagcaaaaac tggcagctat tatcgattat
    18661 tttcatcagt ttcatagcct cagcaaagat gaagtggacg agatactgtt tgcaaccgga
    18721 cgcacctctt taaaaacaag tgaaagtaac ggagtattga tatatagcca cagcggaaag
    18781 gctattaaag cccgtactgt caaccagcat aaatttttaa aggaatacga taaaaacgat
    18841 ttgatttttg ccgttgggcc tgccggaact ggcaaaactt atactgctat tgccctggct
    18901 gtaaaagcct tgcgcgacaa acatgtaaaa cgccttattc ttactcgtcc tgctgttgaa
    18961 gctggtgaaa aactcggatt tttgccagga gacatgaaag aaaaactcga tccatactta
    19021 cagcccttgt atgatgcctt gcatgatatg atgcccatga agcgcctcac tacatatctt
    19081 gaggatggca ctgtgcaaat tgcccctttg gcctttatgc gtggccgcac gctggacaat
    19141 gcttttgtaa tccttgacga agcacaaaat gccactgtaa accaattaaa gatgtttctt
    19201 acccgtatgg ggcgcgatgc taaatttatt gtaaccggcg accttactca gattgactta
    19261 cccagaaaaa gccaatcagg cttgcctttt gcccttaagc tattaagaaa cattgatgga
    19321 atttcggttg ttgaatttga tcagcgcgat attatccggc acccgctggt taaacagatt
    19381 gttgatgctt atgataaatc tgatattcac gaaaatgatt aacggtgatt ttttacaccg
    19441 ttaatgtcat ctgagttaaa tacaaaacca agccttttgc ctgtttgcat ttttgaattt
    19501 tcaatatttt caatcatctg ggctacagta gctatcttta cctggtcata aggcggtact
    19561 aacaaatcaa aatctagggc atattgtatt gaaccaatta caatctttct aatagctgcc
    19621 ctgtctatct tttcagtacc aagcatgtta caagtaaaag ccaactggcc ttttccttca
    19681 accatgtagt aaaagtccct gtttataaat atcctgccga tgaggtaacc caaatcttct
    19741 tcacgattaa atttgaacga gtcggccaga aaattgtaaa tattaattat cccacaatat
    19801 gtatttaaag ggttttgtgc tgcaaaactc gttttttgta tattgtgttc gcggttaaac
    19861 tcaaaaacat tggagtgcat actgaaaata agcaaatctc cggcaatccg caactgtgcc
    19921 tccgctttgc cattgtcttt aaattcaagt aaaattcttt gatctaaacc acttaattca
    19981 tggtttatct cggcagctat ctctgtcaac actgttttca acacagtaaa tacatttgcc
    20041 gtattatcat ggatcatctg tttggtcaat gattttgacc ttaacacctc aagaatttta
    20101 cttttatcgt ccatacaaaa ggtttattag tatgctttgg caaacaatac acggccttca
    20161 gatggttttc cggtataaac gcatttaccc ccctcctttt tctgatcaaa agggattaca
    20221 cgaatagtag ctttggtttc ttccttaatt ttttcttctg tttcagacgt cccatcccag
    20281 tgtgctgaaa taaaaccacc tttttcttct aatacttttt taaactcttc ccaagaatta
    20341 acctcggttg tattagcttt tctataagcc aatgctttat tgtaaagatt tttctgaata
    20401 tcgccaagca aaaatttaac cgtatcaagt atttcagtag cagcataaga cttcttctcc
    20461 aatgtatcgc gacgtgctat ttctactgta ccgttttcaa ggtctttggg gccaatggca
    20521 atacgcacag gtaccccttt taattcatat tcgttaaatt tccagccagg tttatgggta
    20581 tctctatcat caaacttaac agatatgcca tttgccttaa gcattgaaaa tatttcattt
    20641 gctttttctg ctaccttctt aaactcttcg tctgtcttat aaattggaac aataacaacc
    20701 tgaagtgggg ccaatttagg aggcaacaca agtccattat cgtcagagtg ggccatgata
    20761 agcgctccaa tcaaacgggt tgaaactccc catgacgtag cccaaacata ctcctgaaca
    20821 ccttgtttgc tctgaaattt cacatcaaag gctttggcaa agttctgtcc cagaaagtgt
    20881 gaagtaccac attgcagagc tttcccatcc tgcataaggg cttcaataca aagagtatct
    20941 aaagcgcctg caaaacgttc atttgccgat ttatgtcctt taataaccgg aatagccata
    21001 taattttctg caaagtcagc ataaacatca atcatttttc gggtttcaat ctctgcttct
    21061 tctcgtgtag catgagctgt atgaccttcc tgccacaaaa attcggcagt acgaagaaac
    21121 agtcgggtac gcatttccca acgaactaca tttgcccatt gattgacaag gattggaagg
    21181 tctcgatagc tctgaatcca tcctttataa gtattccaaa tgatagtctc cgatgtaggc
    21241 cttacaataa gctcttcttc aagctttgcc tcactgtcta ccactacgcc tttaccattt
    21301 gggtcattct tcaaacgata atgggtaacc acagcacatt cttttgcaaa accttctaca
    21361 tggcttgcct cctttgaaaa aaacgactta ggaatgaaaa gcgggaagta tgcgttggta
    21421 tgtcctgtat ctttaaacat tttgtccagc gaagcctgca tcttttccca tattgagtat
    21481 ccatagggtt taataaccat acaaccacga actgctgaac tttcggccat atcagcctta
    21541 attaccaaat cgttatacca ctgcgaataa tctgtcgccc tgcttgtcaa ttctttagcc
    21601 atcttatgcg ttttaaattc tttctgaaaa agaccacaaa gtaaagaaaa aaagatttac
    21661 agagcaagct ctttagttat tggttgacat tcccccccca taaataactg aagcattagc
    21721 tcataaaact aacgtttgat gagattaaaa atacacttct tgattttttc ttttgcataa
    21781 gggcaaatgt ttgtaataac tgtaattttc aaacacctaa gcattcttct tatgttggaa
    21841 tgacgtatta tttaataaac cctaagattt attttttgct ttattagtcc tctctgaaat
    21901 actactactc caatatcata aaaatcaata catattgtac ttttcgggtg attgcaaata
    21961 taatcccaag cttttcccat atcttttgac caacaaatat catcaaatac aagcatagaa
    22021 tgctcatcca aatgttgact aaacatttcg taatacttca cagtggcttt ataagtatga
    22081 ttaccatcaa cataagcaaa gaatgggctt ttcaaattat caaccaatac tggcagcacg
    22141 tcatcaaact tttgagaaat aagccttaca tttttcaacc ctaatttaac aaagttatcg
    22201 tttgcttttt cagccaaatt cttttcgcct tctactgtaa taactgtttt gtaaggcatt
    22261 gctaatgcaa tcgacgcaga agacaaaccc agagaagtac ccaattctat tgccatttga
    22321 ggttctacaa atttcaccaa ctgatgtaaa attcttaatt ttcgggcact tccagaggct
    22381 tttttacata taccagatat agtatcatac ctaacaaccc ctgtccccgg atcgataata
    22441 tgaataaggg ttttatcgct aagcaattcc tctcttaaca attctacagg ggctaaattt
    22501 ttttgaacat tcttagcatt tagtaaaaat ccatcggtga gttttatcag aaaaggcaag
    22561 ccaaaactat tcacccttaa aggcttactg tgatacaaaa aaccaatagc tgagaatatt
    22621 ctataaatga gtagcaaact tacttgtctt taggttcagt accagcttta ataattttag
    22681 cgctacctgt gatattttgc ctgatttcgg gggccccatt atattcaact ttcccaaatc
    22741 ctgaaatatt gactctcagc atcttttcta catctacgta agctctgcca aaaccagaaa
    22801 tatcaatatc acattgccca attttcaggc taaaagcatt aaggctgcct gtaccggcaa
    22861 tattcagggc taattcatcc gttttgcctt ttaaagtaat ctcagcccct ccactaacat
    22921 tgaccgaaaa cttttttaca tctatctgca aatcaacctt tccggcacct gttatattaa
    22981 gagctaaagc atcccatgaa agcaaactgt ctgaagaaag ctctgtagcg cccgaaaaat
    23041 ctaccgactc aatattgggg gcatatatgt atagcttcaa ctccctcgat cctataactg
    23101 tcttgatatt agaaattttc aaagcactat cttctactat agctacgaca ttttcaataa
    23161 gattttcatc agcaataatt ctcagtcttg aactgtcact atttaccaaa acaacatcaa
    23221 aagcccccat aatagaaacc tggttaaaag cctccagcaa ccttgtttgt tctgttactt
    23281 tcccattgcc cttaatcgtt ttcttttcgc aagaagcaaa aacaaaaaac aacaccagca
    23341 aacaatataa atattttttc atccgtaaat aatttttaag aaaactcgtc ccgaccaatc
    23401 gggattcatc cttgtttgta tttaacaaat catgctcgtt tcattcgaac aaatttgatt
    23461 ttttaaggta aaatgataca aaaaataaac aatactcact tttacaattc gtgtttcatt
    23521 tttaggtaca ccaaaacgtt tacattttat tcatcacttt tagtgatttt aaaatcatgg
    23581 acatttcatc tttttttaca aaattacaat gatttggaaa aatgtgcttc actttaaaat
    23641 ttttattaaa tttattcttc ataaaaaaaa tgatttttca atgccaacat tgacaaatgt
    23701 ataataaact ctatttttca tttgcaaaat acagataaaa atcagtacac aatatgacta
    23761 taaaaacaaa atatctggta aaagccctta ccttgattat gggaatatta cttcagtttc
    23821 aaatgcttga agctgcgccc aaagaccagt ttagatttga gggttcgctt aaaagcagta
    23881 ctgaaggggt agctgatatg attatcaaca tatttgaaaa caacaaaaaa attaattcga
    23941 taaaatcaaa ttcgcaaggg aattttgctt tagaacttga acataacaaa caatatacct
    24001 tggaattgat aaaaacaggg tttatcattc aaaaaattgt tattgatagt aaaatttctc
    24061 caaaattgca agcttcagga ggtatagacc ttgctattga caacgaaata cctgtgtttg
    24121 aaaatgttga aggcttaaaa actgacattt ttgaacaacc tgtaatttat ctgatttata
    24181 acgaaaataa aagctacttt gagcaagacc gtaaacgctc aaaaaacctt tcagcactta
    24241 ccaaccaaat agtttcgcat aaaaataaac tggcaaataa tgaactcaaa aatgccaata
    24301 accttttcaa caaaaaaaaa taccctgaag ccattgaaag ctatttgaaa gttttgtcca
    24361 taagccccaa caaccagcaa gcctctttaa aaattaaaga aatagaaaaa attaccaaag
    24421 acgaacaggc cttagaaaat aaatacaaca aaactatcaa agaagctgat gcccttttaa
    24481 aacaggcaga ctactatgaa gccaaagaaa aatacaaaat agccgttgct atttatcccg
    24541 aaaaaaaata cgctaaaagt aaagtctctg aaatagactc aattatctcg tccaacttaa
    24601 gtaaaaacct aaccctattt gaaaaattga aaaaagatgc cttaaaagcc tatcaaaata
    24661 aagatcttga aaattccctg gcattatata acgaagctaa aaagcttaac ggcgaagaca
    24721 aacaggtgat aaaacaaata gctgctatat caaaagctat tgaagccgaa aagctaagaa
    24781 aagaagaaga actaaggcaa cttgaagaaa agtttacctc atttttgaac aaggccaaag
    24841 attacgaaaa aaacaaagac tggaacaact ctctatcaat gatttcacaa gcactattaa
    24901 tcaaacctga cgatgcagaa tgcaataaaa taaaagataa tgcagaaaaa aacattgcca
    24961 ttgaaaaaca acgccagcag aaagaaaaaa attatcagga tacattagcc cttgcagaca
    25021 aagcagctca ggataaaaat taccaccagg ctatcaaact ttatgagtat gcccaaaaaa
    25081 tgaaacccgg agaaaaatat tcttctgaaa gaattaataa aataaaaaca tttttaaaag
    25141 aattagaagc ttctaaacaa gtttcaccac tatctaaaaa catagctatt aatgattcat
    25201 tgtcaaatga aaagtataaa aacgctgaag attatgaaaa aaaattaaag caactttcat
    25261 ttacaaaaga caaaaaacat tatatgcaaa caatagccag gctaagtata atgtaccaac
    25321 aacaaagaaa gctggacaaa ggtatcaggc tcttagaaaa atccttatta gaagctaaaa
    25381 gaaacaaaga catagaatct ataaccgaaa tacttaatta ccttacagtt ttttactacg
    25441 actctggaat gtataaacaa actatcgatg cttataacca aatgatagat attttcaagc
    25501 aaacaggaaa cctcgaaatg ttggcccata ccctttctga ggcaggcgcc gtactagcct
    25561 ctgtttacca atatgatgct gctattgaaa ccctcgaaaa agccttagaa ttgcataaaa
    25621 aaaatgatga taaaaaaatg gctttgctca catgtcaata tattgcagaa gtatatgcca
    25681 acatggaaga ctcaaaaaat gcaattaaat acttcaaaca agctattgac agggcccaca
    25741 gcatgaatga ccagcaagcc cttggtacgt tgcaaaacag cataggcgta gtatattgca
    25801 aatcaggaaa ttatgatatg gctttaacct attataacca ggcattagaa gtagagaaaa
    25861 aagccaataa catgaaaaat atgtccctca ctttgaacaa tatcggaaat ataaattata
    25921 actggaacga atacgaaaaa gctattgagt attatcaaaa atcgctcaaa ataaaacgag
    25981 aaataaactt tgaagacggc atagctatta cattgtacaa tattggctca acttacatcg
    26041 aattgaaaaa tctttcaaaa gcactggact acctaaactc cagcctcgaa gttgccaaca
    26101 aaacaaagtt tggagaagta atacagcagt cttacaaagc gctatcaaaa gtatatgaaa
    26161 tgaaagacga ctttcgcaac gccgtggctg catacaaatc atatactgac aatgcaatcc
    26221 caggctacaa caacgaaaaa caattttatg aaacaaacga cttctatggc aaagacagac
    26281 aattagtaac taaactaaaa aaagaacttt ataaacaaaa aattttgatt gagaaccaag
    26341 ccctcattaa caaacaaaaa gaacagcaaa ttgccatcca gaaaatggaa atcagccaac
    26401 aaaaatcaaa aaccgccaaa acaaggacgc ttttattatt ttctattctc atcagcctat
    26461 taattggttc catcactatc caaatcttca gaaggtacaa agaaaaaaaa ttgtacagcg
    26521 aagttatcag ttttcagaaa agacaaatta ccgacagtat agaatatgcc agccgcatcc
    26581 aaaaagctgt tttacccccg gacgaagtag tcaaagccca attcccaaac agtttcatat
    26641 acaacaaacc aaaagatatt gttagtggcg actattttta tgtcactgcc attgacaata
    26701 aagtttatgt agcagtagcc gattgtacag gccatggcgt acccggggca tttatgagta
    26761 tgttgggctt atcgttaata aaagaagtaa tacattttag caatgaagcc ccttcggcaa
    26821 ataccgtatt gaacaatctg cgagatgcat taatgaaatc actgcaccaa accggtcgtg
    26881 aagacgaagc acgcgatggt atagatattg ctctttgtgt aattgataaa aacaccaata
    26941 tccttgaata ttcgggggcc aataatccat gctacatcat tcgcgacaat aaaatcatag
    27001 atttaaaagc cgaccgcatg cccattggca tacatcctat attaaacgac ttcaacagcc
    27061 aacagctcga attaaagaaa aacgatgtaa tttatttatt ttctgacggt tatcgcgatc
    27121 agataggaga agaaacttta aagaaattta gaaaagatta cttcaacaat cttttactcg
    27181 aaatacatgc ccttcctatg gaccagcaag cttatatctt agaccaacga catctccaat
    27241 ggcgcgggtc aattgaacaa accgatgaca tactaattat gggcataaaa gtataaaatg
    27301 tagtttcttt gaaaaactct ccttatcatt gcacttacat tttagtcaat gactttacca
    27361 tctggagaat atacgcagga agaactgaat gctttcaaag gtatttttga taaatactat
    27421 gaaagcatta ggaattttgc ctatttcaaa accggagata ttgatgttgc agaagatatt
    27481 gtacaggaaa cattcattaa agtttgggaa attcgtgata ctttagtaga agacactgtt
    27541 aaatcgttgc tttatgccat agccgcaaac agtataaaaa atcatttcag acataaaaaa
    27601 gtagtgttaa attttgccta ttcaaagaac aatgaatctg aaaattcaga atctgctgat
    27661 gctgaccttc aacaacagga aatgcataat aagcttcttt ttatcttaaa tgaaatgcct
    27721 gaaaaatcta aagaagtatt tttactcaac cgcatagaaa agcttactta tcaggaaata
    27781 gccaaacgcc ttgatttgag cgtcaaaact attgaaaaaa gaatgcatca agcattggtt
    27841 catattcgca aacacctaaa ttacaagatt taatggccac tgataatgac atattgaagc
    27901 aatttgggtt agaaagcgac attgaaatac agcgcttttt agaaagtgtt gataaactta
    27961 aagttcctgt atcatctaaa aacagggatg aagcatggtc taaattggaa aattcaatca
    28021 aaacaagaaa aacaaaagaa attaaattca atttcaaaca tgctttttta attgctgctt
    28081 ctatagcttt atttattggt ttatggtttg gctttgagca atggaaccat gctaaataca
    28141 gcacttcatc aggaaaaact cttacttttg aacttccaga cggaagccag gtaatactta
    28201 atgcagaatc acaggtcaac tggaataaat ttcgttggaa atattccaga atagttattt
    28261 taaaaggtga agccttattc aaagtaaaca aaggaagcag gtttgaagta caaacatcac
    28321 aaaacaaagt gtctgtaatg gggacaaaat tcaatgtttt ctctcgccct ggttattttg
    28381 aagttaagtg ttatgaaggt aaggtagctg ttgatattaa aggatcctca caggttattc
    28441 tgaccaaagg gaaagctata aaaaaagaat cttcaagtag tacagctgaa tcatttgaaa
    28501 tacaggaatc agacactaac aactggacaa aaggcgagtt ttattatagc aacgcctctt
    28561 taagattagt attagatgaa attgagcgcc aattcaatgt taacataaat tacactgctg
    28621 aagacaggag atattcaggt tatttcaaaa acaattcatt agattcagca ttgataaata
    28681 tttgtatgcc tttaggatta aaatacaaca ttatccgaga ttctgttgtt atcaggtagg
    28741 gtttttcttc acatatgtta aatgaagaaa agatattatt caaaaaaaac atttatgttt
    28801 aaatggttaa ctcgctattt gctccttttc ttaataatac ctttttcaat caacacttat
    28861 ggtcaaaagg taagcgctga ttattcaaac ttatcgttga cagaggttct gactgcaata
    28921 tctcagcaat accatataaa aattgctttt gacacacaac tagctgacaa aatagcaata
    28981 acccaaagtc tgaagcaaat accaatagaa aaagcgctat cattaattct taacaactca
    29041 gggctgaaag ttcagcgtat gggcgacgtt tacatggtta tacctgattt aaaccctgtt
    29101 aaacgtgtac ctgatggaaa tagtgtacaa cagactgcga ctacaaaaac cagaaaattt
    29161 atttatggcg ccatcaagga tatgcaaagt ggtgaagcac tgccttttgc cagtatatat
    29221 ctgaaaaaca accaccaggg cataacatcc aacgctgatg gtatctttaa actcgaaact
    29281 aaagacgaag atagcattta cttggtaatc aattatattg gatatgaacc gtttgctatt
    29341 aaagccaaag cctcatctca ccctgaactt ttgcaaataa ggctaaatcc gcaattgcag
    29401 caactgcaag ctattgttat aagccctaaa attgaggtat ttgaaaacag gggtactcag
    29461 cccgatgtga taaagttttg tccatcaaaa ctagaaaata tcccttctat taatgagctt
    29521 gacatttcaa cacctttaca aatgctgcct ggaataagtg gttgtaccga aaatgcaggt
    29581 gggtttagta ttagaaaagc gccaactgat aaaaccctta tggtatttga tggttttaca
    29641 ctttaccaca tgaaccattt cttcggagct ttcagcgcaa ttaacacaaa atcaatcaaa
    29701 gatatacaag tacgcaaaat tccgggggat gcaaatttag ggggaagttc ggccggacta
    29761 attgaaatta ccggaaaatc tgggaacatg caagatatat ctgtaaatgc tggtattgac
    29821 atgcttgcta ctgatgttga aattgaaatt ccgattataa aaaataaatg ctcattttta
    29881 tttgccggac gccgctcatt tactgataaa ttccgaactc ctctgtacaa cgccatgttt
    29941 gacaacgccc gctatgaatt tctttcgtat tacagaaaac cacctgttgc attctcttca
    30001 ggcacaggtg atcctcaata ttattttggc gacttcaatt cgaaaatatc aataaacacg
    30061 tcagaaaaaa gtacattatc attgagtgct tttggcagca aagacgattt aaactttagt
    30121 caactggctg cttaccccaa aataaaggaa aacaccaatt ggcaaactaa aggattcagc
    30181 attcgctggg ctggtgatgt taacacttca tggaacactg aattggtaag tggctactca
    30241 aatacaacat ttgattatac tttgtctgac tctatccaga aatttagaaa gcgtttagtg
    30301 aggacggttg aatacattgt atcaaaaggt aacaatattg atttgtatct aaaaaatttt
    30361 tctatccaaa ataacaataa aataaatact tctgaaaaag gtcaactaat atttggagca
    30421 ggtatgcagg ttgtaagctc ttattatgga ttaaatgcat ttgcctgggt aaattcaatg
    30481 aacattcttg acacatcccg catatataaa gaaaattcta aaataacttc agcttggctc
    30541 caatacgaat ggaaaaatga atatttgaca ataaaaccag gcctaagagt taaccactac
    30601 aaacttctca acaaaaatta ccccgaattg aggggaacta tgtttatcag acctgtaaat
    30661 aagctaatat taagagttga agccgggaac ttttatcaat ttgtaaataa agtagacttt
    30721 gtcaggcatg gagactttag aagtgcttgg atattaagtg atggggaaaa ataccctgtt
    30781 gcttcatcaa aaaatattgc acttggagtt aattacaaca ttacatcgcg tttaaatatt
    30841 gatattgaag catactataa tcagatgttt gaccttacca gctctcagac aatatataaa
    30901 attacaacaa acaataacat taagcttgct aatataagtt ggctgtatga taacaaagta
    30961 aagggtctcg acgtgttatt aaaacaagat tggcaatatt accaaatctg gctgtcatat
    31021 accctctctg aggcaaaaaa taaagccccc caaaacagta caacacaaac ttatacggca
    31081 gacaacgacc aaatgcatga agtaaaactt ttgcaattat tcaattataa aaatttctct
    31141 ataagctgga gcaacatcgg aggctcaggt attgtctggg atgagtacat acttgacaat
    31201 aacctgcaat taactcctga ttatcaaaaa aactcagggt cagcaccttt ctattacaga
    31261 atggatgccg gggctaatta tacccttaaa ctatctaaat gtagcctaaa ggctggtatc
    31321 aaccttttca acattttaga caggaaaaac ctgattaata aatttgagaa actgtccgat
    31381 actcccattg ctgatattaa ccaaggccta aaccctcttg aagaaacctc tgtgtatggt
    31441 ttaggagttg cctgtaattt ctttctaaac atttccttct aattactaat atacagtaat
    31501 actgttattt acatcttttg aaatattttt ttcaaaaaag gtagggtttt agttttttga
    31561 agttaactat tatcgaaaag caaattgcta tgtcgtcaga aggaaagaaa tataaacata
    31621 aagacatcaa agcttttgat gaaactttaa aactttatga aaattttaaa attcctgcaa
    31681 aatatagcaa agatgattct tgggaaaaat tcaaaacagt aattgaaaca ccccatgaaa
    31741 aggctcctcc aaaaacacgt tctttgaaag tattttatac atctgctgct gcaataatat
    31801 tactactaat tggcctttca ttgttcaatt atttcaatgc caacatcatt attaccagta
    31861 aaaacggtga aaataaactg gtagtattgc ccgacagctc aactgttgag ctaaacgcag
    31921 cttcaacgct ttcgtacaac caatttggat ggaaacatca cagattagta attttagacg
    31981 gagaaggatt ttttagtgta aaaaaaggaa aacgttttga ggtaaaaacc aatcatggaa
    32041 acgtagtagt attaggcact tcattcaatg tatttgccag aaatgagaat ttctttgtag
    32101 tttgtaaaac aggaaaagta aaagtagaat caataaacac tgttgtgctg actcccggat
    32161 tgaaagcctt caaaaataaa gaccaaaaga taaatataga aaatgcaata gttgaacacg
    32221 aaacagcttg gcaaaacgga gaattctggt tcaacaatgt cagacttatt gatgttttga
    32281 atgaattgga aagacaattt gatattaaaa tagtttataa agatattgaa aacagaagat
    32341 ataccggata ctttaacata aagtcgctcc aagatgcttt aacagcagta tgtagcccaa
    32401 tgcaattgaa gtttaaaaat gaaaatgata aaacttatat tatttattaa caagcaaatc
    32461 aaacaattat gagaaaaatg aattatttaa ccatgatggc cttagttgcc ataaccatca
    32521 actttacatc ctgtaaaaaa gatgatgaca cccaggttag tattactaac gaagatgtta
    32581 ctgtaatgca agatgacgct caaattaatg acatgatgga cgacattgat aatgaagctg
    32641 atgaaatatc tgacgttgcc caatcgaaat cagcattagc aagcgattca acaacatact
    32701 caggcagaga agttgacaga aagctaaata atgacgggac tcgtacaata accattacat
    32761 atacaaactt tcagcaccct aaagcaaaga atgaacgtat caaaaatggc attgtatata
    32821 ttgttgttac cggttcccgg tctgacaata cttataagag aatagttact tttgaaaact
    32881 ttaccatcaa caataataaa atagaaggga ccaaaaccat agaaaaaatg gaaaccctga
    32941 aatacaaaat cactttaact aatggtaaaa taacctttac tgatggttca tctgtaacat
    33001 gcaattacat tcgctatcgt acaatggttg ctggtattgc tacacctctt tacatctggg
    33061 atgatgccta tacatttgaa ggcagcgcct caggagtaac cagcaaaaac atcgaatata
    33121 ccaaaactat taccaaagct gttaaaatat acactgcata tcgctacccg gttgaaggaa
    33181 cattcagttt cacctctggt gacaaaacct tcatatttga ctatggcgat ggcactaaag
    33241 acaacctggc aacaatcact tacaaagatg tatccaaaga aataacatta aaaaaatagc
    33301 atttgattga aaaaaaagcg ccttaaaatt tcggcgcttt ttttttcaag ctttaacaca
    33361 actaacaata tgtcaataaa aacattttga aaagatgatt tttttttaca tcttgcggaa
    33421 aatgaaaaga gagtattttt tgattgaaat gaattaaaaa tttggatgaa acaagcatgt
    33481 ttagaagtaa aaaacaaaaa cgaataaaca aaatcatgcc agttccatcc ggctttataa
    33541 aaataatttt gttcggatga aagaaaaaat tgaattagag tatattttaa aaacatcacc
    33601 caaagtacta tttccaaggt taagcactgc taccggactg gcagaatggt ttgcagacaa
    33661 tgttactgta aaaaacaaaa cacaatacgc ttttcattgg gacaaatcaa ttgaagaagc
    33721 cgagcaaaca tacatccgtg aaaatgttgt tgttaaatat gaatggaaaa acaaagacga
    33781 aaaagccttc tttgaatttc ggatcaggat tgatgacctt accggcgaaa tggccttaat
    33841 agttaccgac atggttgaca gcgatgaaaa agatgaaatt attgacttgt gggatattca
    33901 aataaataag ctaaaacacg tattaggttt ataaacgaaa accattacat ttgcagtttt
    33961 caacccgggc agcaatgaga ataaaaaaac tgcatatatt cattctaaaa agctttatta
    34021 gtcctttatt tgttacgttt ttcatagtca tcttcatctt gatgatgcaa tttttatgga
    34081 aatacgttga cgatcttgtc ggaaaaggtt taggatatga cgttttactc gaactgcttt
    34141 tttttgcctc ggctacattt gtaccattag cccttcccct tgccttgctg ctgtctgcca
    34201 ttatgacttt tggtaacctt ggcgaaaact ttgaacttac agcccttaaa gcttccggca
    34261 tatcactttg gcgaatcatg taccccctta tactgttaat tgcagctatc agtattgcag
    34321 cattctttta ttccaatata tctttaccct atttcaactg gaaattccgg gccttgcttt
    34381 tcgatataca acaacaaaga cctgaacttc agattaaaga aggcgtatat tacaacggga
    34441 tacaaggata tgtaataaaa ataggcgata aagacgtacg caccaaccag ctcaaaaaca
    34501 tcaaaatata tgaccatacc caaaatgacg gaaatacaag cgttactacc gccgattcag
    34561 gctacatcaa aataacacct gacaaacgct tccttgtatt aaccctgtta catggacaaa
    34621 gctatgtaga tatgcccgaa aaaaacagaa gtgaacaaga tagaaaatac ccctttcgcc
    34681 gcgataaatt ctcaaaacaa gaagtcaata tcccactgga aggctttaat tttcaaagaa
    34741 cggaagaagc acttttccgc aataactttc aaatgatgaa cctcaaacaa ctcaatcaag
    34801 ctgttgactc tatgaacaat gaaataaaag cagatgccaa tatgctaaaa tccaacatga
    34861 tgtatggcca tttaacttca tccctctctc ccccaaaaga cacaagcttt aaactcgata
    34921 gtactataaa atttaacaac gtccgcttta tatattatct tttagacaaa aatgaaagaa
    34981 aagcaataat aaaccaagcc ctggcagatg cccgcaacgc taaaaacaac atcaacagcg
    35041 aaactgaagt acgcaggaat aaaatgatac gcttacgaaa acatgaaatt gaatggcaca
    35101 aaaaatttac actttcgtta gcctgtatca tatttttctt tattggtgcg cctctcgggg
    35161 ctattatccg acgtggaggg ctcgggctcc cacttgttat ttcggttgtt ttctttatca
    35221 tatattacat cgtcagtttg acaggcgaaa agatggcacg cgagtcattg tggccagtct
    35281 ggcaaggagt atggttttca tctttaatat taatgcctat tggtatattc ttgactatca
    35341 aagctaccaa cgattcgggt atttttaaag ttgaagttta tcaaaatacc attatcaaat
    35401 tctttaagca agtatggtct taccttaaac ctttatatat cagtatttca aatgttttaa
    35461 gcaaaaaaac tgataacaga tgatgaaaca agcatgttta gaagtaaaca caaccagaaa
    35521 tgaaaatact gattctatct aacaaaccac cctaccctcc aaaagacgga ggtgctattg
    35581 ctatttttaa cctggctaaa ggacttgctg atgccgggca tttcgtccat atactatcaa
    35641 tgaataccag caaacatcag gtaaaacctg aaactatgcc tttattttcc aatatcaggt
    35701 tttcattcgt aaatatagat acttctattc agcctgtaaa agcattgttc aacctgttat
    35761 tctcatctat cccttataat gctgagcgtt tcatatcaaa atacttttta tcccaattaa
    35821 aaactattct tcaggcagac caatacaatg ttgtccaatt agaaggccct tacctgggtt
    35881 attgcattcc tttaatccga aaatattcta atgccaaaat tgttatcagg gcacacaata
    35941 ttgaatgtga gatttgggaa cgctccgccc tgcaagaaaa aaatatcctg aaaaagttgt
    36001 atttatctat cttatcaaaa agattaaagc tgtacgaaaa aaatatcctc cagcaatgcg
    36061 atatcattct gccaatctct tcaagagata atgaacacct taaaaatatt ggaatttgca
    36121 aaccaggcat tgttgttcct gttggtatag atattaataa caattacaat catcataact
    36181 cttctgaaaa ttcaatactt tacattggtg cactagattg gatgccaaac caggaaggca
    36241 ttttatggtt tatcaacgac gtatggccta tcatccaaaa aaaagaaaaa aatgttatac
    36301 tccatattgc cggccgaaat gcccccgagt ggttagtaaa aaagttgtca agtgtcaaaa
    36361 accttctttt ttatggagaa attgaaaact cccataactt catcaaacag catggaataa
    36421 tgatagttcc gctattttcg ggaagtggca tgcgtgtaaa aatcattgaa ggaatggcgc
    36481 tggcaaaagc cattgtcaca acccctgttg gatgtgaagg tattgatgcc gaaaacggca
    36541 aacacctttt tattactgac aaagcagaaa cttatgctga gcatgtcctg tatttattgc
    36601 acaacaatgc caaagctgaa gagatgggaa atgcagcttt tgaatttgtt ggcaaagaat
    36661 atagcaacca gtctattatt agtaagttga ccaactttta taaccaaact ttgtgatgac
    36721 agctgttgca gtgatatttt ggctttgtgt tttggccgta ttttactcat acgtattata
    36781 cccgttaata ttacagctaa tagtttttat aaaaggccga aaaacttaca aaagctacga
    36841 aaaaaatgaa caactccctt caatatctat attaattgca gcattcaatg aagaattagt
    36901 tattgaacaa aagcttaggt ctatatttaa cacaacttat ccaaaaaaca aaattgaagt
    36961 ctggataggt agcgacaact caacagataa caccaatgct attcttgaaa agctaagcaa
    37021 tgaatacagc aacctgcatt ttatcaattt ccacaacaga caaggtaaga taagtatcat
    37081 caaccaattg tctgacaagg ccaatggagc tatactcatc atttccgatg ccaatgttat
    37141 ttttgaagaa aatacactct tcgagctaac cagatttttt gctgatgagc aaattggact
    37201 ggttgatacc cacatgaaaa actatggttt caaaagagat ggcatttcaa tacaagaaaa
    37261 atcctatatt tcttatgaag taaaaattaa aaaccttgaa agctcatcat ttgggacaat
    37321 gattgggccc tttggaggat gctacgcggt acgaaaagaa ttattcacca aagtacccat
    37381 taacttcatg gttgacgact tttttgtttg tatgaaagtc ctcatttcgg gaaaagatgc
    37441 aataaacaac atgaacgcca ttgtatatga agacgtttca aacaatttga atgttgaatt
    37501 caggcgaaaa ataaggattg cagctggtaa cttccaaaac ctcagatatt tcaagcattt
    37561 gatactaacc gtttacaaag gcgtttctta ttgctttata tctcataaag tactccgctg
    37621 gttaggccca tttttcatca ttggggcctt tatatccaac ctgattctgg ccctaaatgc
    37681 aaaattctac ttgatattgt ttttaattca acttatggtc tcaattttac cattggttga
    37741 ttttttatta aaaaaaataa atttacatat cgtaattttg cgcttcgcaa cgcattttta
    37801 caccatgaac ctttcgttgc ttttagggtt cattaaatct ataaaaggga ttaaatcaaa
    37861 tgtctgggaa ccaacacaac gacttcaagg gtaaactaag caccatccct gaagctattg
    37921 aagatatcaa aaacggaaaa attatcatag tggttgacaa tgaagaccgc gaaaatgaag
    37981 gcgattttat ctgcgcagca gaactaatca cccctgaaat catcaatttc atggcaacac
    38041 atggcagggg ccttatctgc actcctcttc ctgaagaacg ttgcgatgag cttgaactcg
    38101 acctgatggt taacaaaaat actgcaacct acagcacagc cttcactgtc agcgttgacc
    38161 ttcttggata tggctgtact acaggcatat ctgcttatga cagggcaatg acggtaaaag
    38221 cattggtaaa tcccgaaaca aggcccgaag aacttggccg cccgggacat atatttcctt
    38281 taagggctaa aaacaaaggc gtactgcgcc gtcctggaca taccgaagct gccgttgacc
    38341 ttaccatcct tgcagggcta aagcctggag gtgtcctcgt cgaaattatg aacgaagatg
    38401 gcactatggc ccgcttgccc gaattagtta aaattgcaga aaaatttgac ttaaaaatta
    38461 tctctataaa agaccttatt gcttaccgtc ttgaacatga aagccttgtt atcggaggcg
    38521 aaaaagtaaa attacctact gcttatggcg actttgagct gattccgttt attcaaaaat
    38581 caaacgggca ggaacacgtt gctattatta aaggttcatg gacaaaagac acccctgtac
    38641 tggttagggt gcactcttcc tgtatcacag gcgatatatt tggttctaag cgatgcgact
    38701 gtggcgacca actacatcat gctatgcaga tgatcgaaaa agaaggaacc ggggtcctgt
    38761 tgtatttaaa ccaagaagga cgcggaattg gcctttttaa caaaatgaaa gcatataagc
    38821 ttcaggaaca aggcatggat actgttgaag ccaatttatt tctcggtttt gaagatgatg
    38881 aaagagatta cggtattgga gccaatatac tgcgcgaatt gggactgggt aaacttaggc
    38941 ttatcactaa caaccctaaa aaaagaatag gtttggaagg ttatggcctt gaggtagttg
    39001 aaaatattcc tattgttgtt tcgccaaatc ctcacaacct caaatacctt acaactaaag
    39061 aagagaaaat ggggcatttc cttaatcttg cccgctatcg taaagactcc ttctaaaata
    39121 cataataaaa tatgggactt aaggtagtat tcatgggtac gcccgacttt gctgttgcaa
    39181 cacttgatgc cattgtaaaa tcaaaacaca aagtactggc tgttgttaca tctcctgaca
    39241 aaccggccgg aagaggcctg caattgcaga tgtctgaagt taaacaatac gcattacaac
    39301 aaagcatccc ggtgttgcag cccgaaaagc taaaagatga atcttttatt gaaaagctga
    39361 aaagcttcaa tgcagatgtc tttgtagtag tggctttccg tatgttgcca gaagtagttt
    39421 ggtcaatacc aaggaaaggc actataaatg tccatgcatc ccttctgcct caataccgtg
    39481 gagctgcacc aataaatagg gctattatga atggtgagac tgtaacagga gtaactactt
    39541 tctttataga aaaagaaata gatacaggac aaatcattaa gcaaaacaaa gtaaatattg
    39601 aacctacaga aaatgcttct tcgttgtatg aaaaactgaa aattgccggg gctaatctac
    39661 ttatcgaaac acttgatggg cttgaaaaca acacattgaa gcccattgga cagcatatat
    39721 tgattgataa aaaaatacct ttaaatgagg ctccaaaaat ttttaaagaa gactgtaaaa
    39781 ttgactggaa caaaagcaaa gaagccatat tcaaccatat acggggacta agcatgtacc
    39841 ccgcagcctg ggctattctg gcaggcccca aaacagccac tgtcaaaatt tacaccgctt
    39901 caatggtaga aagtgtacac aatttgccaa atggaacaat tgaaacaaac aatagaagct
    39961 atctccatgt tgctgtcaac aatggaatga ttgccatcga aacgctccag ctcgaaggca
    40021 aaaaacgcct caatgtagat gagttcttac gtggctttca acatattgaa tcttatcatt
    40081 tcgaataaat ataagcacta tgatgcatac tattctcaat caccgcacta tacgtaagta
    40141 taaaactact cctgtttcac aaaaaatgct tgaagaaata ttactggctg gcacaaaagc
    40201 ctctaacacg ggcaacatgc aaacttatag catgattgtc actactgaca gcaccttaaa
    40261 agaacaactt tgcaaatgcc actttggaca agagatggta aaggttgccc ctgtactcat
    40321 tacattttgt gcagatttca accgttttaa caaatggtgc aacttaagaa aagcaaaacc
    40381 aggatatgac aacttcttgt cttttttcaa tgctgccatt gatgccctat tagtagccca
    40441 aaatgttgcc cttgctgctg aagaaaaagg cttaggtatt tgctacctcg gcactaccac
    40501 ctacaatgca aaacaaatta ttgatattct gaaccttcct aaaggcgttg tcccggtaac
    40561 aacaatagtc atgggttatc ctgaagtaat tccagacctt accgaccgtt taccattgga
    40621 agctgttgtt cattacgaaa aataccaaga ttattccgat gaaaaaatta atgaacttta
    40681 cagcgaaaaa gaagcattgc catttcatca ggaactgttg aaaataaaca acaaagaaac
    40741 attggcacaa attttcacag atattcgtta caaaaaggcc gacaatattg ctttctctaa
    40801 agcatttcta aaagcaattg aagaccaaat gtttatgaat aacgaataaa aggcatggat
    40861 ttactgactt ttattaatag caactgcaaa gaactacttc atttgcttaa tgcagacgaa
    40921 gatggctggc aattgccatt tattgaaagt attaacaaag ctattcatct caaccgatgg
    40981 tttgacaagg aaaatatctt acgaagatta cgttgcacct ttgaatacta cgcctctgat
    41041 gccctaatca atgaccttac agctaatgaa aaggtaattg aacaaaaaac aattggactt
    41101 atcagcgaag aacgcattcc aatggaagag attactacaa ttctgaattt aattaatgcc
    41161 gggaacaagg tcatatacaa aaagcctgaa aactatgaca ttgtcttatg ttcccttttt
    41221 gaatattggc aaaataaagc tggacgcgag cttttgccca tagaatttac agacaggcct
    41281 ataaaaggtt ttgatacgct gcttatcaca ggacgtgaac cttggaaagg tgaaaaaaga
    41341 aagttttttg aaaaacatca tgtcattgaa aaaacacggt acaattccat tggtattatt
    41401 acagactcca taaccaatga acaattcaag gcaatggcca gtgatatatt ttgttattat
    41461 gggcttggct gtggtaacat acgtaaaata tatattcctg aagggtttgc tattgagcgt
    41521 atatttgaag ccattgaacc ttggcacaat gtcatgttgc acaatgcgta tgccaataat
    41581 ttccaatacc atcaatcggt tttcctgatg aacaagatta aacacctcga caatggcttt
    41641 attttgctta aagaagatgc tgcatcttat gccccaattg gaattttgca ttatcaatac
    41701 tattcagata taaataaact gaaagtttta attgataaag aaaataaata ccagatttta
    41761 agcaacaatc ctgagcttgg cctcaaattt ggcaatgcag ataaacagtt gctgaattag
    41821 ttattagttg aaagtaattc gtaattgaac aaaaagtttc tcatagtaat tgccgggcct
    41881 actgctgttg gaaaaactgc ttttgccgtt acggcagccc aatatttctc gacagaaatc
    41941 atatctgctg attcaaggca attttacaaa gagcttagca taggcgttgc acgcccatcc
    42001 gaagccgaat tatctttagt gaagcatcat ttcattgcct cacattctat acatgactat
    42061 tttactgccg gaatgtatga gctatctgtc attgagcttt taaacaggct atttcaaaac
    42121 catgacgtaa ttgtattaac cggtggttca ggattgtaca ttgatgctgt ttgcaatggc
    42181 attgacgacc tgccagatat tgatatggat atccgcaata aagtattgga aagatatgaa
    42241 aatgaaggta ttgagccttt agtagaagat ttgaaaagca tagaccctgc atattatcaa
    42301 acagccgatt tggcaaaccc taaacgaatt tttaaagcgc tcgaaatatt ttacatgaca
    42361 gggaagcctt actcagaatt ccttaccaac ccaaaaaaag aacgaaattt caacatcata
    42421 aagattgtgc tcaacaaagc ccgggaagaa ctttaccagc aaatcaacca gcgtgtagat
    42481 atgatgatgg aaacaggtct attagatgaa gcccgcaacc tatatcctct tcgaaaactc
    42541 aatgcactta atactgttgg atacaaagag ctttttgaat atttcgacaa taaaataaca
    42601 ctaaaacaag ccattgacaa aataaagcaa aacacccgcc attatgccaa aagacaaatt
    42661 acctggttta aacgaaacaa tgattatacc tggtttgatg ctactaacaa agagctgatt
    42721 ttgcaattct tacaaaaaga aatacatgct tagtcaaatt tcttggcctt ttcccagata
    42781 gcattcatct catccaacgt catatctttc agcttcttat gcatatcgct tgactctttt
    42841 tcaaggtatt cgaaacgctt tttaaacttg cggttagtac gttcaagggc tgtctcaggg
    42901 tctataccat aaagccttgc ggcatttacc acagaaaaca ataaatcgcc aaactccgct
    42961 tccaattttt gtttgtcacc actttttatc tcaacctgaa tttcttcaag ctcttccttt
    43021 accttgtccc atacttgttc gggattgtcc caatcaaagc caaccgaacg caccttttcc
    43081 tggatacggt tggcttttac caatgccggt aacgaatgtg gcactcctcc taatacagag
    43141 cgattaccct cccgaagttt caacttttcc cagttgtgct taacttccga atcattagat
    43201 acactcactt caccaaatac atgaggatgc cggaatacaa gcttttcgca aatgccattt
    43261 aaaacatcag taatatcaaa agccccggtt tcagaggcta ttttagcata aaagaccaaa
    43321 tgaagcgcta aatcgcccaa ttcttttttt atctcaggca aatttttctc aataatagca
    43381 tctgccaact cataaacctc ttcaatagta aggtttcgga tagtatcaaa ggtctgtatt
    43441 ttatcccagg gacatttttc cctgagttcg tccataatat caagaagacg ttgaaaagct
    43501 tgtaaacgtg ggtccataat ctaatactgt tcgttttcgt taggaaaatc accagtttta
    43561 acatcagaaa catagcgctg aacagcattg gtaattatat caaaaagatt ttggtaacga
    43621 cgaagaaaac gtggcgaaaa ctctttagtc aggcctaaca tatcgtgcag taccaatacc
    43681 tgcccatcac atccgtttcc agccccaata ccaataatag ggattttaag ctctcctgca
    43741 actttttttg ctaaaacagc aggaattttt tctaatacaa ttgaaaaaca accaattttt
    43801 tcaagcaaat gagcgtcttc tacaagttta ttagcctctg cctcttctct ggcacggact
    43861 acatatgtac catacttatg aatagattgt ggcataaggc caagatgccc cataaccggt
    43921 attccggcag ataatatgcg ttctacagat tcttgtatct ctattcctcc ctcaatttta
    43981 acagctccgg caccagtttc tttcataata cgtatagccg aagctaaagc ttcttttgag
    44041 ttgccctgat atgtcccaaa tggcaaatcg caaacaacta aagctcgatt tacagccctg
    44101 gcaacagagg ccgcatgata tatcatatga tctaaagtta ttggaagagt tgtttcaaac
    44161 cccgccatca cattagaagc tgaatcgcca acaagaataa catctatccc ggcctgatct
    44221 aaaatacggg ccattgaata atcgtaagcc gtaagcatag atattttttc tcctcttagc
    44281 ttcatctcac tgagcttatg agtagtaatc tttttcaagt ttaattgatc tgatatatac
    44341 ataaaagtaa aatcaattag gtttgtcaat aaaatactat aaaatagcat ttaaagtgca
    44401 aagttgctaa ttattttttt actgccatta ataaataggc atattattaa tcaatttgct
    44461 tctccttaat tcaaatctat ttaacaccag caagttaatt tctgcctaca caacaatcaa
    44521 aatattaaaa acaatctcaa gtatatacaa tcaaaacaaa tctgacgttt tgtcatattt
    44581 tttatgtcag agattaaaat ttttatctca tttgtgtgcc ataataacac aaaaagaaac
    44641 tttaataatg ttggcacaaa gcttgataaa ccatctttaa acaagaaaaa gtttaaaatt
    44701 atattaatat gggaaaaatt attggaatcg atttaggaac cacgaactca tgcgtggcag
    44761 taatggaagg taatgagcca gtggttatta ccaacagtga aggaaaacgt acaactcctt
    44821 caattgttgc ttttacagga aatggcgaaa gaaaagttgg agaccccgca aaacgtcagg
    44881 ctattacaaa cccaaaacat accgtatatt ctataaaacg tttcatggga gaaacctacg
    44941 accgcgtgac taaagaaatc cagcgtgaac cttacgacat cgtcaaagga gacaataaca
    45001 cgccacgtgt tgttattgaa gatcgtaaat actcgccaca agaaatttca gcaatgatac
    45061 ttcaaaaaat gaagaaaaca gctgaagatt atcttggcac tgaagttaca gaagctgtta
    45121 tcactgttcc ggcttatttt aatgacgcac agcgtcaagc aactaaagaa gccggagaaa
    45181 ttgcaggatt aaatgttcgt cgtattatta acgaaccaac agctgcagct ttagcttatg
    45241 gccttgacaa aaaacaccag gatatgaaag tagctgtctt cgaccttggt ggtggaacat
    45301 ttgatatttc tatcctcgaa ttgggcgaag gtgtttttga agtaaaatca accaatggcg
    45361 acactcatct tggaggagac gatttcgacc aggttattat caactggctg gcagaagaat
    45421 ttatcaaaga cgaaggtatt gacttacgta aagaccctat ggctttgcaa cgtcttaaag
    45481 aagctgccga aaaagctaag attgagcttt caagcagtac ttcaactgaa attaacctgc
    45541 catacattat gcctgttaac ggtatcccta aacaccttgt taaaaacctc accagggccc
    45601 agtttgaaaa acttgccgat tcattaatcc aggcttgtat tgaaccttgc cgtaaagcgc
    45661 tttctgatgc aggtttgaaa gcttcagata ttaacgaggt gatattagtt ggaggttcaa
    45721 cacgtatccc ggctgtacaa aaaattgtag aagacttttt tggccggacc ccttcaaaag
    45781 gagtcaaccc tgatgaagtt gtagctgttg gggctgcaat ccagggaggt gtattaaccg
    45841 gagaagttaa agatgtttta ctccttgatg ttactcctct ttcattaggt atcgaaactt
    45901 tgggcggtgt aatgaccaaa cttattgaag ccaataccac cattcccacc cgtaagtccg
    45961 aaacctttac cactgcagca gataaccagt cgagcgttga aatacatata ttgcaaggcg
    46021 aacgtccgat ggcaagcggg aacaaaacaa tagggcgctt ccacctcgat ggcatcccac
    46081 cttcaccacg tggtgtacct caaattgaag taagttttga cattgatgcc aatggtattt
    46141 tgcacgtctc tgctaaagat aaagccacag gcaaaagcca aagtatccgt attgaggcat
    46201 ctacaggcct taccgatgaa gaaattaaac ggatgcgaga tgaagctaaa gccaatgaag
    46261 cagcagacaa acaagctaaa gaacgtatcg acaaacttaa tgctgcagac agtcttattt
    46321 tccaaacaga aaaacaactg aaagaatttg gagataagat ccctgctgac aaaaaaggag
    46381 ctatcgaatc agccctcgct aacctcaaag aagctcataa atcgcaaaat attgaagcca
    46441 ttgacaaagc tacagcagaa ctgaaccatg tttggcaagc tgcatcagaa gaaatgtata
    46501 aagctcaggc tcaacaagga caagctggcg gatcttctgc aggagcccag gacaatgcaa
    46561 agaaaagtgg agacagtgaa gttacagatg ttgactttga agaagtaaag taagtttcat
    46621 catttgtaaa ccacccctac ccctccttat aaaggagggg aaatactaac tgaagtttaa
    46681 atatttacat gatttataaa aagaaaaggc tggaaaaatt ccagcctttt cttttatctt
    46741 tctttccaat attatcaatt caaaaatttt acttcaacct gttctgaagc cttgccagaa
    46801 aaatgtttct caagcttaag tatcaattct tcaggtttga taggttttgt aatataatca
    46861 tcgcatccgg cattgataca tttagacata tcgtcataca tagcaaatgc tgtttgagct
    46921 attattttaa ttgcaggctt atattttttt atcaggcgtg tactttccag tccatttatt
    46981 actggcattt tcacatccat caaaactata tctatttgat tgtttttaat acattctgaa
    47041 aacgccctta ggccgttttc aacatgaatc attttaatgc ctgaatggtg taatatttct
    47101 ttcaggtaca tgaaactata gtaatcgtct tctgcaatta aaatagtttt atctttccaa
    47161 tcaaaatttg caatcatctt ttggtttatt tagataacct taatgttatc tacattaaaa
    47221 aagtaaccct gacaaatagc aattttttat gaaaagtaaa attatttaat tatttggcta
    47281 aaagtttgtt ttttcaaaac aaaatatgaa caaactacac ttttgtgaaa aactaatttt
    47341 ttccattctt ttgaaaccct ggtcttgtgg gatttttgtc tataattgag ataaaaagga
    47401 accttgacaa aaaaactgaa ccaagcccta accacagccc aagcaaaagc aggctttttc
    47461 tttagcagaa actgaaaccc tgcaacagca tcaaaaaata acctgcaaaa caaaataaaa
    47521 ggaagataag ccaaaggcag gtttttcaac atcatgtaaa ggctgtttcg gatattgaaa
    47581 aatatcttct ttggattttc ttttggcaat gcccctccgc cataatgaaa tatcaatgat
    47641 tgaggcacgc aaaaaatcct gaaacctgcc caatgtacac gccaacaaag atctatttct
    47701 tccatgtggg caaaaaaatg ttcgtcaagg ccaccaacag ccttgtaaac ctcagaccta
    47761 atggtcagac atgctcctgt tgcccaaaat acttcagcta catcatcata ttggcaatta
    47821 tctttttcaa gatggtcaaa aaccctgcct cgacaaaatg gataaccaaa tatatcaatg
    47881 taacctcctg cagcccctgc atattcaaac atcgacctgt cgtgcacgct aagtacctta
    47941 ggcattaccg ctgcaacatt ttcgtttttg tctaaaaaat caagtaaagg ctctatccat
    48001 cctttagtaa cttcaacatc actattgagc agaatgaaat actttgcttc aattttactt
    48061 aatgcaagat tgtacccgcc agcaaaacca taatttttag aaaattcaac aagcctgatt
    48121 gtcggaaaca tttgttttac atactcctgc gaaccgtcag acgaattatt gtcagcaaca
    48181 acaagctcta catctttaaa tgttgtatgt tcaacaagca aaggaaggta ctggcgcaaa
    48241 aatccaatac cattccagtt taatataacc acactagcct tgtacatccc tgtccagtat
    48301 ttgatatttc caacgacgat gtgtccatag ccaaaactcg ggatgctctt ttatttgctc
    48361 ttcaagaatt tttacgtgct tgttggtgat ttcatattcg ttggtaagcg aggcatcttc
    48421 agtaattggc acaatctcaa actcgtaata accagttttt accggtatca tctttacaaa
    48481 tactactgca gaattaagct ttcgggccat cttttcggga cctaaaaata ccggtgttac
    48541 ctggttcaaa aattgtgttt ggtaatgaat ccgcgagcgt attggtgttt gatctgatat
    48601 aaaacatgac aaggttttta cgttattttg cttgtaagta aaaaggaccc ggggcgcttc
    48661 ttccatagta accagctctg ccccaaactg ggtacgcatg cgcgtcatta ttttttcaat
    48721 atatttatta gataaaggtt tatataacga aataattctg tatggcatat tctcaggggt
    48781 tccaaccagc cattcccaat ttccataatg tgctgttacg gcaacaatgc tttttccttt
    48841 ttcgaataat tcaaaaaata cctcagggtt tcgataccgg atcctttttt tcacctcagc
    48901 atggctaata tgaataagct ttattgtttc taccaatata tgcccaaaat gtttgtaaaa
    48961 tttcctggca ataaatgcaa tctcagcctc cgacttttcc gggaatgatt ttcgcaggtt
    49021 ctcgtaaaca actttttttc tgtagtttgc aacataataa attaatggat ataatacaat
    49081 atctgaaaat acatataata ggcgtaatgg catcaatgaa atcagccaga gtaaaggaaa
    49141 tacgatgcaa tatataaaca atgccattat ttataaaaat acttattgat taaagctaaa
    49201 aactccttat gcgtattatt gtttgatgca ataatttcgc ggccaaacaa ataattactt
    49261 tcacctttaa agtccgacac ctgcccgcca gcttctttaa caatcaaagc cccggcagct
    49321 acatcccatg gattaaggtt atattcgtaa aatgaatcaa aacggccgca tgctacatag
    49381 gccaaatcag tagcagcaga gcctattctc cttactccat gtgagttttg aaaaagatac
    49441 tccagggttc ccatgaaagc ttgcatctgg tcaaaatcat aatatggaaa cccggtagct
    49501 ataagtgaat ctgcaactgt tggagctttg ctcacgtgta tttgcttatt gttcataaaa
    49561 gcgttgcctc ctttccaggc ataaaagcac tcatcagctt tcacttcata cacaacacct
    49621 gccagaagct cttctccatc agccaaccct atgcttactg catgcggagg cattccgtga
    49681 atgaaattgg tggtaccgtc caaagggtca ataatccaat taagggcacc tttagcttct
    49741 ttgccactac cttcttcggc tataaaccca gcctctggca acagtttttt caacccctca
    49801 acaatttttg cttcagcatt tttatccacg tatgtaacaa agttgttaag gcctttcacc
    49861 tcaacatttt ctgctttaat tttttcgcct tcctggttaa taaacaatcc tgtctcgcga
    49921 gccaactggc aaacattaag gcatattttt tctaattgca ttggaaatgt cttttgaaaa
    49981 ttatatgtaa tgcacaaaat taggcaaatt atgaaacaat agaagctttt accagcatat
    50041 tatccaatac ttcgtctaaa gtaacagcag aaagagctgc tatcagcttt ttatcataag
    50101 gtgctgaaac tttgatatag ttcgaggtaa aaccatgcat ttgcccatct tttaacttac
    50161 tttcaaatat cacagtctct ttgcggccta tattttcttg tataaacgct tcttttttta
    50221 cattaccaag ttcaataagc gctttgctcc tgcgttcttt aacctgtggc gatagctttc
    50281 ctttcatatc aaaggctttg gtattaggcc tttccgaaaa aggaaataca tgcaggtaac
    50341 ttacaggcaa cgacttgata aactcaaatg catccatgaa gtcatcatct gtctcacccg
    50401 gaaagccaac aatgacatca gctccaatgc atgcgtaagg cataacctct tttattgcct
    50461 taacacgggc tgcaaaaacc tcacgtttat aacgacgccc catggcagca agtattttat
    50521 cgcatcccga ttgcagaggg atatgaaaat gatgggcaaa acggtttgag ctttttacaa
    50581 agtcaataat ttcatcatgt ataagattcg gctcaatgga agatatgcga tatcgctcaa
    50641 ttccttcaac cttatctaat tgttttacaa gctcaaagaa gttttcgccg gtagtcttgc
    50701 caaaatcgcc aatatttaca ccagtcaaga ctatctcttt agtatttttt gctgctattt
    50761 cttttgcccg agatactaca cttgagatat cctcgttcct gctcgaacct cgtgcaagtg
    50821 gtatagtaca atatgaacaa tgatagtcac agccatcttg aaccttcaaa aaagcccttg
    50881 ttctgtcaga tatggaatac gatgcttcat aagaatttac actggcaata tcacacgagt
    50941 gtattttaac agcattgcca ttccacaagc tttcaacatg ctctacaagc ttaaacttat
    51001 ctgcagtgcc caataccagg tcaacacctt ctatagccgc tatttcttca ggtttcaatt
    51061 gcgaataaca gccaattaca actacttttg cacctttaga tgtagccttt tttattgcct
    51121 gtcggcattt tttatcagca acctgggtta ccgaacaggt atttatgaca aatacatctg
    51181 cctcggcatc aaatgacact ttttgataac cactttgctc aaatttacgc tgaagcattg
    51241 acgattcggc ataattgagc ttacaaccca aggtataaaa cgctactttt ctcatcattt
    51301 ttaacaaaaa aggacattaa ataaatgccc ttttgctctg aaataaaact atttttttta
    51361 tattttcatt tctattgtga ctccggaggg attagtttcg ttgcacttca tgacccctcc
    51421 taatgatgtc gcaatcaagt ccctttcaaa ccaaaagaag aaacatttct acttctcctt
    51481 tctattgtga ctccggaggg attagtttcg ttgcacttca tgacccctcc taa
//
LOCUS       DAOSPF010000020        50035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_742_length_50035_cov_7.008764, whole genome shotgun sequence.
ACCESSION   DAOSPF010000020 DAOSPF010000000
VERSION     DAOSPF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 50035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_50035_cov_7.008764"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK252_05770"
     CDS             complement(<1..244)
                     /locus_tag="PK252_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14255.1"
                     /translation="MKCYIMLTTGTFGFSVEIGQKAMKKNFSFKASPVSLKLFYIWPD
                     VNGVKKKALSAKRGQLFEHSELLPGAVDKAFFSQFTA"
     gene            complement(383..988)
                     /locus_tag="PK252_05775"
     CDS             complement(383..988)
                     /locus_tag="PK252_05775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT14256.1"
                     /translation="MNTEIENKRFVESIEPANINLLPMARFEGKIEVISHIAEVKSAC
                     EYLSSYKLLGFDTETKPSFKKGKSNKPCLLQLSNHEIAFIFRLNKTGLTDELCHLLAN
                     KNITKIGTSLKDDFRELQKLGHFTPNSFIDLQSFVDQFGIKDKSLKKLTAIVLNLHLS
                     KSQQVSNWENEVLTPQQQIYAATDAWVCLEIYNELLKYQTL"
     gene            complement(966..1571)
                     /locus_tag="PK252_05780"
     CDS             complement(966..1571)
                     /locus_tag="PK252_05780"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOT14257.1"
                     /translation="MLKELVFSKNVVEFTAVANEYCHFLENVSSNEKAEFIDKSIKLL
                     SLMYYKALSLPNAELVNEEPNETFVTEFDWHFIRNGIQSLLDEDDIYFEMYDENLQEL
                     SEPESNTISEAMADIYQNVKDYLEIYKLGNEELSNDAIVNITETFNEYWGFELVKSLK
                     TLHILRKHTSDNLSEQPKNKPLSDKKNWFISQAQDEYRDRE"
     gene            complement(1607..2224)
                     /locus_tag="PK252_05785"
     CDS             complement(1607..2224)
                     /locus_tag="PK252_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT14258.1"
                     /translation="MEGKLPEKTVERLSEYRRSLLNCLLEGKTYIFSHELARLHNITA
                     VQVRRDIMFIGYSNAQRKGYEIKEMINVIGKVLDSNDGINVAIVGIGNLGRAITSYFS
                     GKRSKLNIVAAFDNDPDKIDTIIAGVPCFHISQLGQIVKEKNISIGIITVPSDYAVDT
                     AKSLISVGIKGILNFTSVPLNVPESVYLENSDMITLLEKVAYFVK"
     gene            complement(2359..3195)
                     /locus_tag="PK252_05790"
     CDS             complement(2359..3195)
                     /locus_tag="PK252_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14259.1"
                     /translation="MKHRWVVICMFLFLPSFAYKGICSLTPSSLSNEINTLLDSIKNI
                     PGDSLRKNVNDKVISILDKYFRQATSFNDSLIGMKYIGKIFSPDKKFNIITWNLPMQD
                     GTNTFYGFIQINPKKDSVCKVYFLKNNNLFDPKALPYVKTNTDRWIGALYYEIVPPLN
                     KKNDTYILLGSQLNNMFTSKKIIETMTIDEDNIVFGSPVIYIGQKPQCRIIFEFAISA
                     KMSLQYIADKQMIVFDHLSPVSPVYVGNYKFYGPDFTYDGLKYEKDRWNYVSDIFLRN
                     KK"
     gene            complement(3226..3933)
                     /locus_tag="PK252_05795"
     CDS             complement(3226..3933)
                     /locus_tag="PK252_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT14260.1"
                     /translation="MNCIIIDDDKLSRKVVEEFIQKTESLELTGSFSNAPDAIQFLKQ
                     NDQTNLIFLDIEMPDMSGLDFLDTMKKVPQIIIMSSKGKYALNAFEYDVTDYLLKPIQ
                     YARFYKAIDKAMNRMQRNSIAAPENEEIFIRKNSALVKLKCDDILFVEALENYVIFNT
                     QNEKYTIHFTMKLVEQKLPSNKFVRVHRSYIVNSRCFNEIEDNSIIITTGEGRKIIPI
                     GKSYRDNLMNALNLISK"
     gene            complement(4123..4806)
                     /locus_tag="PK252_05800"
     CDS             complement(4123..4806)
                     /locus_tag="PK252_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT14261.1"
                     /translation="MSESLNTFLKQFVNENRLKLIDQKLQYRTRHIAVVLENLHQSHN
                     ASAILRTCECLGIQDIHIIENSNNFQPNDEIALGASQWLTLHNYNTQTNNTTWAIQQL
                     KKQGYTIAATSLHTASVPIDEYPIEDKPTAFVFGTEVTGISEDIEKLADCFVSIPMVG
                     FTESLNVSVAVGIVINQMLTRLKKNSADWMLSPDELEELELQWLRNSIKHVKLLEKHF
                     YETVKKEKF"
     gene            complement(4887..5162)
                     /locus_tag="PK252_05805"
     CDS             complement(4887..5162)
                     /locus_tag="PK252_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT14262.1"
                     /translation="MNKAQLIDAIAADAKLTKADSKKALDAVIKATTVALKKGDRVAL
                     VGFGSFSVAKRSARTGRNPQTGKEIKIAAKKVVKFKAGSDLTSAVQK"
     gene            complement(5576..6406)
                     /locus_tag="PK252_05810"
     CDS             complement(5576..6406)
                     /locus_tag="PK252_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOT14263.1"
                     /translation="MAYKLLKGKRGLIFGALNPKSIAWQVAERAFEEGAHIMLSNAPV
                     AKRLGEVEELAKACNSEVIYADATNLDDLEMLISASVEKLGGKLDFVLHSIGMSPNVR
                     KDIPYDNINYDNYLKTLDVSALSFHKVLQTCRKLDAINEWGSVVALTYIAATRTLYGY
                     NDMADAKAALESIARSFGYIYGREKKVRINTISQSPTLTTAGSGIKGFGSLYDFSERM
                     SPLGNATAEECADYAITLFSDLTRKVTMQNLYHDGGFSSMGMSEEAMTQYDKSFEECK
                     "
     gene            6684..9134
                     /gene="pheT"
                     /locus_tag="PK252_05815"
     CDS             6684..9134
                     /gene="pheT"
                     /locus_tag="PK252_05815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT14264.1"
                     /translation="MKISYNWLKQYIDTQLSAEELKTILINLGLEVESMERWQSVKGG
                     LGGFIIAEVLTCQKHPNADKLSVTTVNTGSGEPLQVVCGAPNVAAGQKVILATIGTKI
                     YSGDEVFEIKKSKIRGEASEGMLCAEDELGLGSSHDGIIVLPDDVKVGTPANQYFNVE
                     DDIVFEIGLTPNRIDCASHYGVARDLAAFFNQNNPKKLIKPSVEAFKVDNHNLPIEVV
                     VENQEACIRYSGVSISGVTVKESPDWLKNRLNAIGLRPINNIVDVTNFILHELGQPLH
                     AFDAAHIIGNKVVVRTLPQNTEFVTLDGARRKLDANDLMICNTQEGMCIAGVFGGEKS
                     GVTVATTKVFIESACFHPVWVRKTARRHGLNTDSSFRFERGTDPNITVYALKRAALLI
                     QELAGGAISSEIVDIYPKKVENFKVDITYNYIDTLIGKVIDRDRIKKILEGLEITIAS
                     ETSEGLSLLVPPYRVDVQRPADIVEEIIRVYGYNNIEFSEKVISTLGFTERPTAEQLQ
                     NSISDYLTDNGFNEIMCNSLTASAYYENIEEYKNNIVKILNPLSSDLNCMRQTLIYGA
                     LESIVLNINRKQNNLKMYEFGNTYFFDANKGEGLKKYNEKYHLGLFVTGLDQETNWNT
                     AKEPVGFFHLKGYVNNLLIKAGVNIAQVVAEPVNHPLISNGLQYSINGKLLAVLGSIK
                     SAVLKSFDIKQEVFAAEVEWDLVFSLAKKKKVEYKELPKFPEVKRDLSMILDMQVTYA
                     QIEQLAYKTERKLLKNVNLFDVYQGDKIEQGKKSYALSFILQDEEKTLTDNQIDKVMQ
                     NLIKAYESQLGAKVRMQL"
     gene            9136..9654
                     /locus_tag="PK252_05820"
     CDS             9136..9654
                     /locus_tag="PK252_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOT14265.1"
                     /translation="MAQIKVIKDDITRQQVDVIVNAANSSLLGGGGVDGAIHRAAGPA
                     LFAECKAHNGCKTGDIKVTKGYQLPCKYVFHAVGPIWQGGEHNEEELLKSCYLKSLKH
                     ATELKAKSIAFPNISTGVYHFPKEQAAEVVLKTVQCFFQEQKSSLSEVYFVCFDDENF
                     EIYNRLLPKYIK"
     gene            9654..10520
                     /locus_tag="PK252_05825"
     CDS             9654..10520
                     /locus_tag="PK252_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOT14266.1"
                     /translation="MSVNEVSKYLSLVKFSHTLFAMPFAFIGYFLALKYGDSYFSLKL
                     LLLVVACMVFARNTAMAFNRYADRHIDLHNPRTALREIPAGILKAESVLVFTIINAVL
                     FIATTWFINPMAFYLSPVALFVITGYSLTKRFTVLCHFILGLGLSLAPIGAYIAVTAH
                     FHWLPILFSFIVLTWVSGFDIMYALQDERFDNDWKLKSIPAKLGSRRALILSILLHTI
                     SAFLVVLAGTLAHMNLLYWIGAGVFITLLIYQHLIVKPNDISKVNLAFATTNGIASVV
                     FAAFAIADMLFT"
     gene            complement(10513..11283)
                     /gene="yaaA"
                     /locus_tag="PK252_05830"
     CDS             complement(10513..11283)
                     /gene="yaaA"
                     /locus_tag="PK252_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOT14267.1"
                     /translation="MSFLVVISSAKTMTDKAKKKFATFTQPEFLNEAKKINHTLRELN
                     APQIESLMQINPQLARLNLDRIHNWHTDTNQNNALQAIFSYTGEVYRGVNVQSYTEQD
                     LLFAQDHLRILSGLYGIVRPLDLILPYRLDMGTSFSVEKYKNIYEVWQNKVTASLEKH
                     MKEKKINTLINLASNEYFSVLDTSKLSCKIITPVFYELSGDQLKTIVVYVKKARGLMT
                     SFIMKNRIDDPDDLKNFNQAGYSFYPEVSDENKWVFVR"
     gene            11486..12157
                     /locus_tag="PK252_05835"
     CDS             11486..12157
                     /locus_tag="PK252_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14268.1"
                     /translation="MQKILAFIAIFVMLVSCNTINFSDQPQGVIEYEVIYLTNKSGMP
                     TNLLPRKVVLKFRSNKSITTIEGFMGLFSLSNITDFRKHTNSILLKVMDNRLVYEGKK
                     NEPPFFLSKYFDCSIAITNEKKVLAGLPCYKAIISVPNNKEKKIVYFTQAIPLKNANK
                     ASPFASLEGILMEFDLSLTNIEMHLKATKYIQTEVDAAIFKVDDSYKEVSQRKIISIL
                     NKLLE"
     gene            12233..14674
                     /locus_tag="PK252_05840"
     CDS             12233..14674
                     /locus_tag="PK252_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT14269.1"
                     /translation="MAKEKDKKPKEDTKEDKKGIFTDIRFKAIAGILLILFSVYLTVA
                     FVSFLFTWKYDASFTWQNVFSGSEIEVANWAGKTGARIANWLMSEWMGYAAFGIPIIM
                     FVGGTRMLGFKRFKFRKTALVVLFGMIISSLLFGYFSSIIESFSNGTKIFGSGPGGAH
                     GYFVSHWLNALLGKVGTGFMLLLIAFTYTALIFKPFAQWFVQTVLDMFTHKPKEVPAE
                     AEGTAMTDGIAENMDIPAPPVIENPTEDITKQLNNDELEVIFQKPGDEDVLSTPEPED
                     FDLEDEDDTELELVEGAEEETTDAASLLQELGPYDPTLDLSNYKYPTIELLEDHKSNN
                     PEVTQDELISNKNKIVETLKNYKIEISHIKATIGPTVTLYEIIPAPGVRISKIKSLEN
                     DIALSLAALGIRIIAPIPGRGTIGIEVPNRNPETVSMRSVVSNKKFQESNADLAIVLG
                     KTISNDTYVVDLAKMPHLLVAGATGQGKSVGLNAILTSLLYKKHPSELKLVLIDPKKV
                     ELTLYSKLEKHFLAKLPDTEEAIITDTQKVINTLNSLTIEMEDRYSLLKKAHVRTIKE
                     YNTKFKERRLNPEHGHRFLPYIVLIIDEFADLIMTAGREIETPIARLAQLARAIGIHL
                     IIATQRPTTNIITGMIKANFPARIAFRVISMIDSRTILDSPGANQLIGRGDMLVSTGS
                     DLIRVQCAFVDTPEIEKIVDFIGNQPGYSSAFLLPEVKAEGEDGSISSEDLSSRDSLF
                     DEAARLIVMHQQGSTSLIQRKFSIGYNRAGRIMDQLEVAGIVGPSEGSKARQVLVVDE
                     MSLEHILKRIDGRNN"
     gene            14740..15417
                     /locus_tag="PK252_05845"
     CDS             14740..15417
                     /locus_tag="PK252_05845"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT14270.1"
                     /translation="MISKRYIVAALFIFIAVSYFDTIKAQKDPEAKKIVEQFVRKNKG
                     LDAIFMKFTINTENHQNKDKTSINGEALLKGDKYKLNINKTVIYNDGKLVYSFLPDEK
                     EVSISNFNPQKEENMMVNPAYLFKIYDKNYKYRLLGETTFNSKNCYEIDFYPNDLNKK
                     YSIVKLIISKEKLSLEALKVKMKSGIDYIIKVQSFEEKKNISDSEFVFDTKAHPDVEV
                     VDLRKKK"
     gene            15426..17081
                     /gene="recN"
                     /locus_tag="PK252_05850"
     CDS             15426..17081
                     /gene="recN"
                     /locus_tag="PK252_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT14271.1"
                     /translation="MLKKLTIKNYVLIDDLSISFDNGFSTITGETGAGKSILLGALSL
                     LVGQRADTDVLLDKTSKCIIEAVFDVEQYAIQSFFQQNEIDYDVQTIIRREILETGRS
                     RAYINDTPVNLSVLKELGDKLIDIHSQHQNSYLSDQVFQLKVIDTMAHNAALLSEYRE
                     QFRKYHECKKQLIELTEKVEKDKAELGFLQYQFDELVGANLKAGEQEELENEAIVLSN
                     AESIKIALLNISHYLQAEENGIEVQMKEALNIAQKVKNIFHKAENIAERLGSMYVELK
                     DLSAETERYAEQVELDPERLTLVKERLDMLYSLSQKHKVTSSEELIGLRDKIGERLSN
                     IQSFDEHLALLHKQIEDFCASLHEISLKLREKRKAIAPSLENTVSQMLRQLGMPNAVF
                     VVQFDELNDFQSSGLDSVQFLFTANKQIPAQDISKVASGGEISRTMLAVKSAIASSLA
                     MPAIVFDEIDTGVSGEIADKMGGIMQAMSANMQVISITHLPQVAAKGQYQYKVFKTES
                     SKATNTHIKRLTNDERIIEIAKMLSGKDVSEAAITNAKVLLQS"
     gene            17186..17259
                     /locus_tag="PK252_05855"
     tRNA            17186..17259
                     /locus_tag="PK252_05855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17220..17222,aa:Arg,seq:acg)
     gene            17503..18009
                     /locus_tag="PK252_05860"
     CDS             17503..18009
                     /locus_tag="PK252_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HOT14272.1"
                     /translation="MIKYIYIGVFLVLNLVQIEVYSQQDLANTKKSWLYTSGIWFSKT
                     FNNFDTVFVKNTPSKYSLILRNSNWLNFYNFDMDNKNKIVMQSELYYNAGFILGYKFL
                     LLGYSVNLNETFSGQTVKSQELDLHVNCSCLGIDVYYTDNQGATSITEFKGIKNIKNK
                     GVSFDLMA"
     gene            18012..18536
                     /locus_tag="PK252_05865"
     CDS             18012..18536
                     /locus_tag="PK252_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HOT14273.1"
                     /translation="MRTLDGDINYYFNNKKYANSAAYSRGYFKQQKKSAGSFIAGLSY
                     ARHRIYFDFTNLPEDTMYSSLKTLDDKSILYNSYCLNFGYGYNLVFSKKWLANLTVIP
                     SAGFNSYKKKMSEDIPNNKISMNTKVKAALVYNMSDYFLGFSAIFSADWFFADNYTLV
                     NSFGNFNISYGMRF"
     gene            complement(18607..20757)
                     /gene="dcp"
                     /locus_tag="PK252_05870"
     CDS             complement(18607..20757)
                     /gene="dcp"
                     /locus_tag="PK252_05870"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HOT14274.1"
                     /translation="MIKNISILLTGVLLISLAACRSNSERRTTMEDSVNLASNPLMHV
                     STLPFQAPDFTKIKNSDFAPAFDEGFKEQRAEIDSIANNPEQPTFENTLVALEKSGQL
                     LRRVNAIFNMLAGANTDSVLQKLNEEVSPKLAAQTDAIFLNDKLFERVKKIYQKKNEL
                     KLDIESAKLLEFYYFKFEQSGADLSSQDKEKLKKLNEEEAGLSAKYTNQLLAAAQAGA
                     YFTNNKETLKGLAEGTLNSASQDAEAAGKKGQWMLPLQNTTQQPLLQSLENRQTRQQL
                     FEASWSRAEKGDKNDTRQTILRIAEIRAEKAELLGFPNYAAWNLQDQMAKTPEAVRDF
                     LKKLVPAATAKARKEAADLQALIDKEKGGFRLQPYDWDYYAEKIRKERYDLDETQLKP
                     YFELYTVLEKGVFYAANQLYGLTFKQRNDIPVYNSDMRVYTVYDKDGSELALFYCDYF
                     KRDNKSGGAWMSNVVEQSKLLGTKPAIYNVCNFTKPAPGEPALISFDDVTTLFHEFGH
                     ALHGMFADQTYPSLSGTSVARDFVELPSQFNEHWALEPKVFSNYAIHCKTGKPMPKDL
                     VEKIKKSQTFNQGYAFTEILAAASLDMEWHTIKAGDAIKSVDEFETNALKRTNLALEQ
                     VPPRYRSSYFLHIWGNGYAAGYYAYSWAEMLDNDAYQWFKENGGMTRANGQRFRDKIL
                     SRGNTEEYNQMYYDFSGRKPDIKPMLKKRGLLEE"
     gene            complement(21035..23530)
                     /locus_tag="PK252_05875"
     CDS             complement(21035..23530)
                     /locus_tag="PK252_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14275.1"
                     /translation="MKYKIFPVAIMLLACYTTNITAQDEVINDLAEKIKTYHQEYPEE
                     NIYFTTDKEVYKPSENIWFRAQVVSDNMPSGLSKGLKVKLFDNKGTVVADEKFRVDSS
                     LAVGNIALPKKLSDGYYTILAHSTWMENDSLNHIFRKFIEIRNNKASNYSISIKKINE
                     YFSAKEPIKLEVTVSSNDLQPVADAKVKLELFADNKKSYKETQKTDASGKFVFTIPAE
                     SFTTPIFIKCTSKYKGNESTANTVIYPSPSKIRLSFFPEGGKILSEFAKQVVIKSTDE
                     YGNYVAVSGEIKDSKGPFSTVFKTDKNGLGSVIVASKDNSTLKATLTSPQGNSSEYIL
                     PVKTSGTSMSLIKKTKDTLMIRLENVNNTEASDLYCIAYKAGKIYWASKFGNSKAGMF
                     RVPVNDVPAGVVHVTIFNNKKEVISSLSTYIDHNRTIIASIKPEDNNTAFSIKVNDNK
                     GTPVKGSFSISVYKQNENILGYDTRNFNTYLMKNDFSEYKIDSLSQENTTIDMLLISS
                     LPQFPFLNSSLKKVKYSPKDGISGFVTNYSEKPIPKLQLSLLNKKTLEVATATTDSSG
                     YFICPDINQSVGTVDYTITPTLFKKDIPINILLKSKFDENIKSTAKNTVMTDYLYRNY
                     PIFSNEANSEDMLYDPTLNAKRNKYNTIDQNKYNSSKTVLELIAEKKSYTINENKMFF
                     ANAVRSFKFTIGVMFVIDSVLTSDDITVVKNLNSSDIAEIRILTDPVEVSRYSSFAPN
                     GIVEIITKKGNSNKPVINNEYKVKEFAAVQPFKASPTSNTIFWCPNIMLDAEGKALVK
                     LDNTNVKGRYVVNIAGFTEDGIPFYKKHEFDVK"
     gene            complement(24632..26419)
                     /locus_tag="PK252_05880"
     CDS             complement(24632..26419)
                     /locus_tag="PK252_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT14276.1"
                     /translation="MFKQKLKLSEKIGYGFGDAASSMFWKLFGMYIMFFYTDIFGISA
                     AVVGTMFLITRIGDAFFDPFVGIVCDRTETRWGKFRPYLLWFALPFGIIGVMTFTTPN
                     FDVTGKIVYAYITYSLMMLIYSMINVPYASLMGVMTADGKERTTLATFRFIFAFGGSF
                     LVLGLFQPLFNGFGTDTIGKFSNGNVIEVKDSTQTKVIEKNFIWSGTTQKEQDETVVD
                     SLLYVTAKASIKSAEPIKICVINQSTKDTGCFTFADGKDTLGLMRNGAWSNIELKLSS
                     FKGLDSFSDSAQWQVLVTTAGEADLKLEKIAIKQIDYRSGTQKAVIIIAIIAVIFFLL
                     TFLMTKERIKPLEEKTSLKDDLKDLGKNIPWFILLGAGISTIFFNTIRDGAAVYYFKY
                     YFKGASDITYDSFNMTLAISTIYLLLGQAANIVGVILAKPVSDKIGKKSTFLSAMLFA
                     AVLSIIFFWVDRSNLTLILVLQVLISINAGIIFPLVWSMYADTADYSEWKNGRRATGL
                     VFSAASMSQKIGASIGIALAGWLLAIYGYQANVEQTAETQNGIRMMLSIWPAIGAILA
                     AVFMFIYPLNEKKLEQIEDDLNERRSKKA"
     gene            complement(26440..27066)
                     /locus_tag="PK252_05885"
     CDS             complement(26440..27066)
                     /locus_tag="PK252_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT14277.1"
                     /translation="MKKAQLFLILYFVVILACFIIMAIFSKADIHLFINSLNNNIADH
                     FFKTITNIGDGLFVVIIGVLFLLYRTRVSVNIISTYIISGLAAQIIKRLIDAPRPIAF
                     FEGTAHLHLVEGVKMATVHSFPSGHTASIFALSLCLAFAWNKKITQVIWLLLACIVAY
                     SRMYLSQHFLIDVTAGSIIGITTTLIFTYFSDKWNYAWLDKPIYSIKN"
     gene            27198..28217
                     /gene="galT"
                     /locus_tag="PK252_05890"
     CDS             27198..28217
                     /gene="galT"
                     /locus_tag="PK252_05890"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT14278.1"
                     /translation="MKFEGKWEKRWHPLLQEWVILAATTSERPWSGDTVKNIQAEEPA
                     FDPKCYLCPGVTRASGVTNDNYTEPWAFTNDFASFSLDAPDVHRKSKFETVEPVHGTN
                     RVICYSPVHNVTLAEMPEEQVAKVVALWQKEYATLSVNPKIENVLIFENKGRAIGVSN
                     PHPHGQIYATNFVPRIVERQRQSFADYYRENKSHLLVDLLADELSHKKRVVYENDHFV
                     AFIPFFARFVYETYVVPRRQVARITDLNEAESKSLANMHKVMLSKFDNLYNMSFPNIT
                     MLQNAPCNGHPDNELFHFHIEFYPPLRSPDKLKYLAGFESGGGNIINPVLPDEAALAL
                     RNAKY"
     gene            complement(28517..29665)
                     /locus_tag="PK252_05895"
     CDS             complement(28517..29665)
                     /locus_tag="PK252_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT14279.1"
                     /translation="MLSIKIFFQSRKFFAPAFLYTCFSLIFSTWVTYIPYIAEKINIT
                     EGKIGGAIFFSSLGSFFMIPITNRLVDKIGVGRYAFYAFILYTFALINIFLAKNYAML
                     CAALFCLGFCSCAFAISLNSLTATIEKRAGKYIMTGSHGFWSIGGIIGASTGSFLAGL
                     LKMPLLHVSLLVVVLLSVHIWLRKEYYDIRTEAVEKEKYKKFPFKPLLAIASIGLIMM
                     VSEGAIADWSALYLKKITQVKVQYIGLGYALFAVGMTIGRFNGDALSNRIGSWKLLRL
                     AIGTSLAGFTLVLLTFSVTAFIGFFIIGLGFSIIVPEIYRLASTIKGVKTADGVSFIA
                     ATANIGFMTGPVLLGFIAELHSLHLSFIVLTCFVLLAFVMAIIRAKAA"
     gene            29920..31803
                     /locus_tag="PK252_05900"
     CDS             29920..31803
                     /locus_tag="PK252_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14280.1"
                     /translation="MQPELVPIKIKKGFTLFTLDQSISAANDTVIFLPVNQDFYITKT
                     SSIIATNFFLSLKQKAFKRRWSKSINNVVISTPDSIGPDDTIKVQKAEIPFINYQNLI
                     IRDINVSKLNIFGPSFNNPNDIPRNVFERWVNRTHVTTREYFIKQYLLFKKNSRLDPY
                     QLAESERLLRELPFIEDARVYIYPTSSPDSVDVEVVTKDTWSLGFDFTTANIKDYKLD
                     VWNQNLMGTGSELDNVYFKNSKKIPVDGLKGSYTIRNISGSFINTLIGYEKFGNESFR
                     INFWRDFFTQQTRYAGQVYFENIEKRYVSVEQDTGGYVFFPLKGTVSRLWLGRAFKLP
                     TPLISATTKTSFSIAGGLYQYDYTKAPLVRERYRYEFQNRTFYLLSYSLASYGYYRSS
                     LVYNFGRTEDIPYGLLIKTTQGLEVGEFGNRYYHGVSFLNGGPLLSLGFFYFDAAVGG
                     FIKKKEVEQGVLNVGGNYISNLLVIGRIKARAFINVDFIKGFKRYEDEYLDINNFSGV
                     RGFYNDSARGGQRLVINMESVFFTPWYAGGFRFAPFAFADFAYVGSTRQWIFRNNLYS
                     GFGIGLRLRNEHFVFKTFQIKLAYYPFLSKKASGDLFTLSQEWRFRPNDFTVRSPEIL
                     IYK"
     gene            complement(31804..32388)
                     /locus_tag="PK252_05905"
     CDS             complement(31804..32388)
                     /locus_tag="PK252_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14281.1"
                     /translation="MKIKIFILSALFSASLFGQTTKDTTKVEKTKSKFAENFIFGGFF
                     GLQFGSATYIGLSPMAGYYITPKIIAGIGATYEYYYEKWYSKSISSSIYGARVYGQYT
                     FIDEIGKSTRYKTNMGFFGQVEYEAINLDRDFSDAEKLSKTNRYWLHGVLVGGGIKQS
                     IGKHSSFNISVMFNILEDKRSPYSNPVARVGLYF"
     gene            32493..33194
                     /locus_tag="PK252_05910"
                     /pseudo
     CDS             32493..33194
                     /locus_tag="PK252_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(33272..35329)
                     /gene="ligA"
                     /locus_tag="PK252_05915"
     CDS             complement(33272..35329)
                     /gene="ligA"
                     /locus_tag="PK252_05915"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT14282.1"
                     /translation="MSKEQIALEIEKLRKTLREHNHRYYVLHQPVIGDQEFDQLMKQL
                     QQLEAENPEFFDPTSPTVHVGSDISNEFEQKEHQYPMLSLDNTYNEAELLDFDKRVGK
                     GLGGQQYSYSAELKFDGISISLIYENRQLAYAVTRGDGVKGDIVTNNVKTIRSIPLKI
                     ASATAPANFEIRGEIFMPRAVFEALNEERREAADEPFANPRNAAAGSVKLLKSAEVAK
                     RKLDCYLYNLYTPVPISDSHYQSLQMAAGWGFKISPYSKKCDTIKDVLAYINYWDQKR
                     DSLPFDIDGIVIKVDSLAQQSELGFTAKIPRWAISYKFKAEQVTTELLSVSYQVGRTG
                     AITPVANLKPVQLSGSTVRRASLHNADQIQLLDLHEHDIVCIEKGGEIIPKVTGVEKH
                     SRASDAKPVVFISRCPECNAQLVREEGEAKHFCPNADSCPPQIKGKIEHFISRKAMNI
                     ETLGEGRIDTLYEKGIIKNVADLYYIKYGQIIGLEKTYNAEDGSKPRIVRFQDKTVNN
                     ILQNIEASKQTPFERVLFALGIRHIGQTAARKLAQHFKNIDAIKNSTIEQLTAVDDIG
                     EVMAQSVLAWFSVPNNAIIVERLREAGVQMQIKEDTNKVVSEKLKGKSIVVSGSFSSP
                     QRRKELEQMVIANGGKLVDSVTSKTSFIVAGENMGPSKLQKAKELNIDVIDEKTFLNF
                     LEY"
     gene            complement(35405..35779)
                     /locus_tag="PK252_05920"
     CDS             complement(35405..35779)
                     /locus_tag="PK252_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14283.1"
                     /translation="MTTLQIEKTSNTPKVTFDPQSGVMRLEGRSIPENPGDFYKPLVL
                     WLIDYFKSPQKKTIFELKFEYINSGSSKSLLEMFRLIKEQHISGNECLVKWYYEEDDE
                     SVQELGEHYQFTLKIPFEFEAY"
     gene            complement(35825..37066)
                     /locus_tag="PK252_05925"
     CDS             complement(35825..37066)
                     /locus_tag="PK252_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14284.1"
                     /translation="MNFGILNENTRIAIRSIKANMLRAILTMFIIAFGIMALVGILTA
                     IESIKSTLTTQFSTMGASAFAIVKKGNNMHHGGHRERTVNTANITYDQATEFVGRFQF
                     PATVAISGYVSGASTIKFQMQKTDPNIEIWGVDEYDMITSGKSIGQGRNFSHEEIENG
                     KHVIIVGSDVVKKLFKKNENPINQTISVGNGKYRIIGVLAQQGTSFNGSDRLCLIPIT
                     NARQYYPNPDQTYRINILPAKPELLDVAISEAEGIFRIVRRLAPHDANDFEIESSNSL
                     ANMLINNLRLVTMAATIIGIITLFGAAIGLMNIMLVSVTERTSEIGIRKALGAKTSTI
                     KQQFLLESVIIGQMGGALGIILGILIGNMVAMLTGGSFIIPWVWILLGVLLCFVVGVI
                     SGYVPAQKAAKVDPIISLRHE"
     gene            complement(37144..42048)
                     /locus_tag="PK252_05930"
     CDS             complement(37144..42048)
                     /locus_tag="PK252_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT14285.1"
                     /translation="MIFLKILDKYRKISFSERDKGDRFERLMQAYLLTDPQYAHQFRK
                     VWLWDEFPGKDDMGGADTGIDLVAVTHFDEYWAIQCKCYQETATIDKPAVDSFLATSG
                     REFRGEDSKTTSFAQRLWISTTNKWGPNALEAIKNQKPPVTRLNLHDLIEAPVDWEAL
                     EQGIHGQKARVEKKKIYPHVFEVRDKVVEYFKENERGRLIMACGTGKTMTSLKIAEKQ
                     TNNKGTILFLVPSIALIGQTLREWASQAEEPINPICICSDPEITKNKNSADQDLTSTI
                     DLAWPASTDANYILKQFEHYKQNGNAAMTVVFSTYQSIEVIANAQKILLKNGFPEFDL
                     IICDEAHRTTGYTEPGMDDSAFVKVHDENFIKAKKRLYMTATPRLYSIDAQSQAAKQD
                     IPLWSMDNEQYFGKEIHRIGFGEAVEKGLLTDYKVIILTLNDKDVPPAVQNMITNGET
                     EIKTDDLTKLIGTVNALSKQFLGNEAIKTDGDESPMKRAVAFCGKIPDSKNIAASYNL
                     AAENYLDSLPREKKEKMVTVHARHMDGTMAAPQRDQMLAWLKEDTHNNECRIITNVRV
                     LSEGVDVPSLDAVLFLSARNSQVDVVQSVGRVMRRAEGKKYGYIIIPVIVPADVEPEQ
                     ALDDNERYKVVWTVLNALRAHDDRFNATVNKIELNKKRPGQIIVGGADTSFDNDGTPI
                     DKRQGEKQSSETNKEIGRQIAIQFEQLQDVVFARLVQKVGDRRYWEQWAKDVALIAER
                     QIERIAYLITEKKDQREAFDKFLSGLQKNINPGINQEQAIEMLAQHIITQPIFDALFE
                     GYSFVKSNAVSVAMQSMIEALEKGNNLAEQDETLQKFYGSVRKRAQGIDNAEGKQRII
                     IELYDKFFKTAFPKMVERLGIVYTPVEVVDFIIHSVNDILQKEFGRTLSDDNIHILDP
                     FTGTGTFMVRLLQSGLIGLKDLERKYRHEMHANEIVLLAYYIAAINIENAYHDATPDP
                     DDGVGKARYTPFEGIVLTDTFQLGETDDTDKLFSEMFPRNSERVQRQQKAPLRVIIGN
                     PPYSIGQKSANDNAQNQAYPRLDQRIADTYAKLSTAGLNKSLYDAYIKAFRWSTDRLD
                     KTGGVICFVSNGAWLDGNSTDGFRKAIEKEFSSVYVFNLRGNQRTSGELSRKEGGKIF
                     GSGSRTPIAITLLVKNPDIKTDKATIYYHDIGDYLGREQKLKIVKGFKTFANPKLPLK
                     TLQPNGHGDWISVRNSLFDEFIPLEPEKKFDAKSQSVFIAQTLGTATNRDAWVYNFSK
                     ATLCENVKKSIAYYNEQLDLILKQKNKEIIKDVTKGNWTRDWDNQIKRRKEIIENEYE
                     YRITMHRPFTKLNSYFDDDLNQERYQLPKLFPTKNHKNIVISLCGTGTNKDFSALITD
                     VVPDLQLQANGQCFPLYYYEENKVVQKSLFDSGEAAAGGTAAGKYVRRDAVSDFILER
                     ARAQYGKSVSKEDIFYYVYGFLHCKEYRETFAADLKKMLPRLPLVESVADFWAFSKAG
                     RQLAELHLNYETVAPYPHVTVSGDDGQTYTVEKMRFPKKDQKDTILYNSHITVSNIPE
                     KAYEYVVNGKSAIEWVMERYQVTVHKESGIKNDPNDWAAETGNPRYILDLLLSIINVS
                     VQTVDIVNSLPKLTFETGAQEPGK"
     gene            complement(42429..45152)
                     /gene="ppdK"
                     /locus_tag="PK252_05935"
     CDS             complement(42429..45152)
                     /gene="ppdK"
                     /locus_tag="PK252_05935"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT14286.1"
                     /translation="MTTKRVYFFGAKNADGKADMKNLLGGKGANLAEMCLLGLPVPAG
                     FTVTTDVCTEYYANNCELPKELYDEIWANMKKVEDVMGMKYGDPENALLVSCRSGARA
                     SMPGMMDTVLNVGLCTATIPGFIKKTNNPRFVWDSYRRLIMMYADVVMEKAEGIEPKD
                     GKGIRKQLDDMLDDFKHKRGYKSDTEITADELKQLSEDFKKKVKEVLGKEFPDDAKEQ
                     VIGGIKAVFKSWNGKKAVSYRRIEGIPDSWGTAVNVQAMVFGNMGDTSATGVAFTRNP
                     ATGENKFYGEWLVNAQGEDVVAGIRTPSPLNDDTKNEQNKHLKSMEEAMPSTYKELVE
                     IRNTLEKAYKDMLDIEFTIQEGKLYMLQCRAGKRTGTAALNMAMDMLAEGLIDEKKAV
                     MRVEPAQLDELLHPICDPAEEKKVSVLTKGLPAGPGAAVGKIVFTAEDAVAWHRKGEK
                     VILLREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGSLHVNVEAK
                     TAKIADTDIVLKEGDVVTLNGTKGNVYLGALKLMDASENPRFQTFMKMVDKYRTMGVR
                     TNADTPSDAKIARDFGAEGIGLFRTEHMFYGEGSDQPLFLLRKMILSNGVEERKAALD
                     ELFAFVKKDIKATVLAMDGCPVTFRLLDPPLHEFVPVGAEKQAELAKALGISVEAIKK
                     RGEALHESNPMMGHRGVRLGVTYPEVSEMQIRAIFEASAELSKEGKDPHVEIMVPVTC
                     DVSELDFTKKIVDRVYAEVCAKMGVSKINYKYGTMIEIPRAALLSDRMAKTAEFFSFG
                     TNDLTQMTFGFSRDDIGGFLHDYLDKKMLAADPFQTIDQDGVGQLIAMSVQKGRATRP
                     DLKVGICGEQGGDPASVEFCYKNGLTYVSCSPFRVPIARLAAAQAAIKAGK"
     gene            45553..45636
                     /locus_tag="PK252_05940"
     tRNA            45553..45636
                     /locus_tag="PK252_05940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45587..45589,aa:Leu,seq:cag)
     gene            45751..45834
                     /locus_tag="PK252_05945"
     tRNA            45751..45834
                     /locus_tag="PK252_05945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45785..45787,aa:Leu,seq:gag)
     gene            complement(45976..46048)
                     /locus_tag="PK252_05950"
     tRNA            complement(45976..46048)
                     /locus_tag="PK252_05950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46013..46015),aa:Lys,seq:ttt)
     gene            complement(46211..46283)
                     /locus_tag="PK252_05955"
     tRNA            complement(46211..46283)
                     /locus_tag="PK252_05955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46248..46250),aa:Lys,seq:ttt)
     gene            complement(46417..46881)
                     /gene="pyrI"
                     /locus_tag="PK252_05960"
     CDS             complement(46417..46881)
                     /gene="pyrI"
                     /locus_tag="PK252_05960"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOT14287.1"
                     /translation="MHSKDKTQLKVSALRNGTVIDHIPAQNLFKVISILKLEKIKTQI
                     TFGTNLESKQLGSKAIIKISDVFFQQEEISKIALVAPEAKLNIIKEYEVVEKKLLVAP
                     DEVKGIARCFNPKCITNNEAITTRFEVLTKKPVSLKCHYCEKIMHAEHLVLI"
     gene            complement(46887..47795)
                     /gene="pyrB"
                     /locus_tag="PK252_05965"
     CDS             complement(46887..47795)
                     /gene="pyrB"
                     /locus_tag="PK252_05965"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOT14288.1"
                     /translation="MKNKSLVSINDFTQEEHLKLIDLAFQFEKEPPLDKLRNKVVATL
                     FFEPSTRTRLSFESAVTRLGGRVIGFSDASSSSVTKGETLKDTIKIVSSYCDLIVMRH
                     PLEGAARFASEISNVPIINAGDGANQHPSQTLLDMFSIKKTQGKLENINLMMVGDLKY
                     GRTVHSLLMAMNKFNTTFYFISPPELKMPDEYKMLLAQRGMKYYEHTDMAEYIDKVDI
                     MYMTRVQKERFSDPIEYEKTKNAYILNNEMLRNTKDNLKVLHPLPRVNEIDVDVDDNH
                     KAYYFDQARNGVFMRQAIICSILGLI"
     gene            complement(47973..48668)
                     /locus_tag="PK252_05970"
     CDS             complement(47973..48668)
                     /locus_tag="PK252_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOT14289.1"
                     /translation="MEIQILQLIEGAKKAKGLTVVIDVFRAFSLACYVMDHKGTRIFP
                     VGKLEEAYAIKEQHPDFILIGERNERIPEGFDFGNSPTHISQYDFDYKTVIHTTSAGT
                     QGIINAVNADQIITGSFVNAEAIAKYIQKQKPDVVSLVCMGYSAQRPTEEDTFCAEYI
                     KQYLEGSKPDFEQMKKTIRQTSGQRLFLPENQAHSPSSDFDMCLDLNRFSFVLKAEYN
                     GEIKQIELHKILI"
     gene            48771..49847
                     /gene="buk"
                     /locus_tag="PK252_05975"
     CDS             48771..49847
                     /gene="buk"
                     /locus_tag="PK252_05975"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT14290.1"
                     /translation="MYRKLILVINPGSTSTKVAVYHNNSPLFLKNIKHNAEQLKDFPS
                     MSAQYEFRKNFITRELGHAEIDLCDIGIIIGRGGLIKPIESGIYEVNESMYNDLLNSP
                     FGQHASNLGGLIAYDMAKALKGVRAIIADPVVVDELSDVARVAGHPEFKRYPIFHALN
                     QKAIARNYANSHDQKYEELHLIVAHLGGGISVGAHEKGRVVDVNNALDGEGPMSVERS
                     GTIPAGQLVKICFSGKYDQQTIEKMIVGQGGLVAYLGTNDGYEIELMAREGNEKAKTL
                     QNALSYQVGKAIGEMAAVLKGNVDAILLTGGMANNSMITDYIKSMVKFIAPVIIYPGE
                     DEMKAMADNALRVLRGEITPKKYI"
BASE COUNT        14207 a         9637 c        10011 g        16180 t
ORIGIN      
        1 ttgccgtaaa ttggctaaaa aaagctttat cgacggctcc gggcaacaac tcgctatgct
       61 caaacagttg ccctcgcttc gccgataaag cttttttctt aacgccattt acgtcaggcc
      121 aaatgtagaa aagtttcaat gaaacaggcg aagccttaaa agaaaaattc tttttcatcg
      181 ccttttgtcc gatttcgacc gagaagccaa acgttcctgt tgtaagcata atgtaacatt
      241 tcatagcttc gaggttgaaa gagttaaggc gataagagtt tttgcacaac ttttttcgac
      301 aaaaaagttg tataaaagag atgtatttat agctattcta ttgacaaaat gaatttatat
      361 tgaaaatcaa caaaatagga gtttacaacg tttgatattt taaaagctca ttatatattt
      421 caaggcaaac ccaggcatct gtagctgcat atatttgttg ttgcggggta agtacttcgt
      481 tttcccagtt cgatacctgt tgcgacttgg acaaatgcag gtttaaaact atagctgtca
      541 gctttttcag gcttttatct ttaatgccaa attggtcaac aaaactttgt aagtcaataa
      601 aactattggg tgtaaaatgt cctaatttct gcaactccct gaaatcgtct tttagcgagg
      661 tgcctatctt ggtaatgttt ttattggcaa gaagatggca tagctcgtct gtcaggcctg
      721 ttttgttcag cctgaaaata aaggctattt catgattgga caactgcaac aggcagggtt
      781 tattggactt ccctttttta aacgaaggct tagtttcggt atcaaaccct aatagtttat
      841 atgatgacag atattcacat gctgatttta cttcagcaat atggcttatt acttctatct
      901 ttccctcaaa cctggccatt ggcaacaagt taatatttgc cggctcaatg ctttcgacaa
      961 accttttatt ctcgatctct gtattcatcc tgtgcttgtg aaatgaacca attttttttg
     1021 tctgataaag gtttattttt aggttgttca gataggttgt ctgaagtatg tttacgcaat
     1081 atatgtaagg ttttaaggct tttaacaagt tcaaaacccc agtattcgtt aaaagtttca
     1141 gttatattaa ctatcgcatc attagatagt tcttcgtttc caagtttata tatctccagg
     1201 taatctttaa cattttggta aatatctgcc atagcctcag agatagtgtt cgattctggc
     1261 tccgacagct cttgcaggtt ttcatcgtac atttcaaagt aaatatcgtc ctcgtcgagc
     1321 aggctttgta tgccattgcg gataaaatgc cagtcaaatt cggtcacaaa ggtctcatta
     1381 ggctcttcat tgaccagttc ggcattgggc aatgacaaag ctttgtaata catcaacgaa
     1441 aggagtttga tgcttttgtc gataaactca gctttttcgt tcgacgaaac attctcaaga
     1501 aaatgacagt attcattagc tacagcggta aattctacta cattttttga aaatacaagt
     1561 tctttcagca ttttggacaa atttcaacag tacaaaggta aagtttttac tttacaaaat
     1621 aggcaacttt ttccaaaagg gttatcatat ctgagttttc cagataaacg ctttccggaa
     1681 cgttaagagg tactgatgta aaattgagaa taccttttat gccaacagat ataaggcttt
     1741 tggcagtatc aactgcgtaa tcgcttggta cagttataat tcctatactg atatttttct
     1801 cttttactat ttgtcccagt tgtgaaatat gaaaacaagg aactcctgca atgatagtat
     1861 ctattttatc agggtcattg tcaaatgcag caacaatatt aagttttgac ctttttcctg
     1921 aaaaatacga tgtaatagcc cggcctaaat taccaatacc aacaatggcc acgtttatcc
     1981 cgtcgtttga gtcgagtact tttccaatga cgttaatcat ttcttttatt tcgtaccctt
     2041 tacgctgggc attgctatac cctatgaaca tgatatcgcg ccttacctgc acagcagtaa
     2101 tgttgtgtaa gcgggcaagt tcgtgcgaga atatatatgt tttcccttca agcagacaat
     2161 ttaagagcga acgtcggtat tcacttaatc gctcaacagt cttttccggt aatttacctt
     2221 ccatttagtt ttgttttttg ttctgttctt agttttatgt tttcaaagat aatttttttt
     2281 atcagatgta taaaaataca ttcgtttact caatattttt ttgaaattga ggtttatttt
     2341 caaagtgtta tgtgaaaatt attttttatt acgaagaaaa atatcggaaa catagttcca
     2401 tctgtctttt tcatatttta acccatcata ggtgaagtct ggtccgtaaa atttgtaatt
     2461 gccaacatat acaggcgaaa caggagacag gtggtcaaaa acaatcattt gtttatcagc
     2521 aatgtattgt aatgacatct ttgcagatat tgcaaattca aatattatac ggcattgtgg
     2581 cttttgacca atatatataa cggggcttcc aaatactata ttatcttcgt caatagtcat
     2641 cgtctcaata atttttttac ttgtgaacat attattgagc tgtgagccaa gtagaatata
     2701 agtgtcattt tttttgttta atggaggcac tatttcgtaa tataatgctc caatccacct
     2761 gtctgtattg gttttcacgt aaggtaatgc ctttgggtca aacagattat tgtttttcaa
     2821 aaagtaaact ttgcaaacgc tgtctttctt gggatttatc tgaataaacc cgtaaaaagt
     2881 gttggttcca tcttgcattg ggaggttcca ggtgatgatg ttgaattttt tatcgggcga
     2941 gaaaattttt ccaatatatt tcatcccaat aaggctgtcg ttgaatgatg tagcctgtct
     3001 gaaatatttg tccagtatgg aaattacttt gtcgttaaca ttttttcgca ggctatcacc
     3061 gggaatattt ttaatactat ccagcaaagt atttatttca ttgcttaatg agcttggcgt
     3121 taagctacaa atgcctttat aagcaaacga aggaagaaat aaaaacatac aaataacaac
     3181 ccatctgtgt ttcataccaa ataatttgtt ctgcaaataa acaaattatt ttgaaatcag
     3241 gttaagggca ttcatcaggt tgtctcgata tgatttgcct atggggatta ttttgcggcc
     3301 ttctccggtg gttatgataa tactgttatc ttctatttca ttaaaacaac ggctatttac
     3361 aatatatgac cggtgtacac gcacaaactt gttggatgga agcttttgtt ctaccagttt
     3421 catggtaaag tggatggtat atttttcgtt ttgtgtgtta aaaattacat agttttctaa
     3481 cgcttcaaca aataaaatat catcacattt cagcttcacc aatgccgaat ttttgcggat
     3541 aaatatttct tcattttccg gggcggcaat actgttgcgc tgcatacggt tcatagcctt
     3601 gtcaatagct ttgtaaaaac gggcatattg aatgggcttg agcagatagt ccgtaacatc
     3661 atattcaaag gcattgaggg catatttccc ttttgaagac atgataataa tctggggtac
     3721 ttttttcatg gtgtcaagaa agtctagccc agacatatct ggcatttcta tgtctaagaa
     3781 tatcaggttg gtttggtcat tttgttttaa aaattgaata gcgtcggggg cattagaaaa
     3841 actaccggta agttcaagac tttcagtttt ctgtataaat tcctcaacta cttttctcga
     3901 caacttgtca tcatctatta ttatgcaatt catatcagaa atgtttttaa cagaaacctg
     3961 catttgtcat aaaaacattt taaatgcggc ttatatatca aagatagcta tttttttgac
     4021 aaattgtatc tgttgaaata tttttagtaa gaaaagatgt aaataatatc taaacactta
     4081 aaccaggtca tacctcagat ataagcaaca tgataaaggt ctttaaaact tttctttttt
     4141 aacggtttca taaaaatgtt tttccaatag tttgacgtgc tttatcgaat ttcttagcca
     4201 ttgcagctcc agctcttcta attcatcggg actgagcatc caatcagcgc tgtttttttt
     4261 caagcgtgtc agcatttggt tgatgacaat gcccacggcc accgatacat tgaggctttc
     4321 ggtaaagcca accataggaa ttgatacaaa gcaatcggcc agtttttcaa tatcttctga
     4381 gatgcccgta acttcagtgc caaagacaaa agcggtaggt ttatcttcta tcgggtattc
     4441 gtcaataggt acagacgctg tgtgtaatga ggtagcagct atggtgtagc cttgcttttt
     4501 taattgctgg atagcccagg tagtattgtt cgtttgcgta ttgtaattgt gaagcgtgag
     4561 ccattggctg gccccaaggg ctatttcgtc gtttggctga aaattgtttg agttttctat
     4621 tatgtgtatg tcttgtatcc ctaagcattc acatgtacgc aaaatagcgc tggcattatg
     4681 cgactggtga aggttttcta gcactacagc aatgtgcctt gtccggtatt ggagtttttg
     4741 gtcgataagc ttcagccggt tttcgttgac aaattgctta agaaatgtat ttaaagactc
     4801 gctcaagggt ggtgttttta tttgaatgat aaaataaaaa aaaaggagta ccgaagcact
     4861 cccctttaaa agacaatgtc tttttcttac ttttgaacag cagaagtaag atcgctacca
     4921 gctttgaatt taaccacttt cttagcagca attttaattt cttttccagt ttgaggattt
     4981 ctacctgttc ttgcgcttct tttagcaact gagaaagaac cgaaacctac taaagcaact
     5041 ctgtcacctt ttttaagagc aacagttgta gctttgataa ctgcgtcaag agctttcttt
     5101 gagtcagctt ttgttaattt tgcatctgct gcaatagcat caataagttg tgctttgttc
     5161 ataacattaa tttttttagg gttagaccta tttttgttta aaacgtaata caaatatata
     5221 gcatttatta gcatttgcaa attttatgac aagaaaattc ttaccaaagt cactgacaga
     5281 gaggcttttg acttaactta aagaaataga tatagttgca taagtaattg taaataaaca
     5341 atttatcacg caataaaggg gtgccaaggg ttgttgattt ttttttaata aaaatgtaaa
     5401 aaaaatgcta aaaaaatttt ttttagatgc ttttaacaat ggtttgtgga gaattttttc
     5461 aaattggaat acttgaagaa tgaatttttg agcatttttg aaaataaaaa aggccgtcaa
     5521 tgcatgttaa cgacctttta ttcatttttt ataccttata tattatatgg tatatttatt
     5581 tgcattcttc aaaactttta tcatactggg tcatagcttc ttcgctcata cccatgcttg
     5641 agaaccctcc atcgtgatat aagttttgca tggttacctt acgggtaagg tcagaaaaga
     5701 gcgtgattgc atagtcggca cattcttcgg cagtagcatt gccaagaggc gacatgcgtt
     5761 cagaaaaatc gtacaatgaa ccaaaacctt taatgccact tccggctgtt gttagggttg
     5821 gagattgtga gatggtgttg atgcgcactt ttttctccct tccataaata taaccaaaac
     5881 tacgggcaat agactcgagt gcagctttag catcggccat atcgttgtac ccatagaggg
     5941 tacgcgttgc tgcaatgtaa gtcagtgcta ctacagagcc ccattcgttt attgcatcaa
     6001 gcttacggca tgtttgtaaa actttatgaa atgacaatgc agaaacatcc aatgtcttta
     6061 agtaattgtc atagttgata ttgtcgtaag gtatgtcttt acgaacgttt ggcgacatcc
     6121 ctattgaatg tagcacaaag tcaagtttcc caccaagctt ttcaacagat gcgctgataa
     6181 gcatttcaag gtcatcaagg ttagtagcat cagcataaat tacttcgctg ttgcaggctt
     6241 ttgcaagctc ttctacttca cccagacgtt tagcaactgg agcattggat aacataatgt
     6301 gtgcaccttc ttcaaatgca cgttctgcta cctgccatgc aatagatttt gggtttaatg
     6361 ccccaaaaat caaccctcgt ttgcctttta aaagtttata agccataata tattgattaa
     6421 attaaactta gcttttaaca atccgtgttt tcttttgttc aattttggct gcaaaggtaa
     6481 aaatctttac tacatttttt gcttaatctg aaaaatgatg cctaaagagc tttttaaaca
     6541 gggaaaagct ttcaactttt ttgtacattt gcccattgtt aaatttttgt tgttacatga
     6601 agcgagcatg attcgttaaa cacaaacaag gatgaaaatt catcatgcga gttccgtccg
     6661 cccttattta taattattta cggatgaaaa tttcatacaa ttggctgaag caatatatag
     6721 atacccaact ttcagctgaa gaattaaaaa caattttaat aaaccttgga cttgaagtgg
     6781 agtcaatgga aagatggcag tctgtaaaag gcggtcttgg aggttttatt attgccgaag
     6841 tgcttacctg tcagaaacac cctaatgctg ataaacttag tgttactact gtcaataccg
     6901 gtagtggaga acctttgcag gtggtttgtg gtgctcctaa cgtggcagca gggcaaaagg
     6961 taatacttgc caccattggc actaaaatat attcgggtga tgaagttttt gaaatcaaaa
     7021 agtcaaaaat aaggggagaa gcctcagaag gcatgctttg tgccgaagat gagctggggt
     7081 taggcagttc gcacgatggc attatcgtat tgcctgatga tgtaaaagta ggaacgccag
     7141 caaaccaata ttttaatgta gaggacgata ttgtttttga aataggcctt acgcctaatc
     7201 gtatcgactg tgcttcgcat tatggcgtag cccgcgattt ggctgctttt ttcaatcaaa
     7261 ataaccccaa gaaacttata aaaccaagtg ttgaagcttt taaagttgac aaccataatt
     7321 tgccaataga ggttgtagtt gaaaaccagg aggcttgcat tcgttactct ggtgtctcta
     7381 tcagcggggt cactgttaaa gaatctccgg attggttgaa aaacaggtta aatgccatag
     7441 gccttcgccc gataaataat attgtggacg ttaccaactt tatcttgcac gagttggggc
     7501 agcctttgca tgcttttgat gcagcgcata ttattggcaa caaggtagtt gtacgtacat
     7561 tgcctcaaaa taccgaattt gttacccttg atggggccag gcgcaagctg gacgctaatg
     7621 acctgatgat atgtaatacc caggaaggca tgtgtattgc cggggtgttt ggtggtgaaa
     7681 aatctggtgt tactgttgcc acaacaaagg tttttattga aagtgcctgc tttcatccgg
     7741 tttgggtaag gaaaactgcc aggcgccatg gtctgaacac cgattcttct ttccgctttg
     7801 agcgtggtac agaccccaat attactgtgt atgccctgaa aagggctgca ttgcttattc
     7861 aagagcttgc cgggggggct atttcttcag aaatagtaga tatttatcct aaaaaagttg
     7921 aaaattttaa agttgatatc acttataact atatagatac cttgataggc aaagtaatag
     7981 acagggatag aattaaaaaa atattagaag gccttgaaat tactattgct tcagaaacat
     8041 cagaagggct gtctttgttg gtcccaccat acagggttga tgtacaaagg cctgcagata
     8101 ttgtagaaga gattatccgc gtttatggct ataacaatat agaattttct gaaaaagtta
     8161 tatccacact gggttttacc gagaggccta cagctgagca gttacagaac tctatttcag
     8221 actacctgac cgacaatggc tttaatgaga ttatgtgcaa ctcgcttaca gcttctgcat
     8281 attatgaaaa cattgaagaa tataaaaaca atatagtaaa aatattgaac ccgctgagtt
     8341 ctgaccttaa ttgtatgcga caaacattga tttacggtgc acttgagtca atagtgctca
     8401 atataaaccg taaacagaac aacctgaaga tgtatgagtt tggaaacacc tatttctttg
     8461 atgcaaataa aggtgaaggc cttaaaaaat acaatgaaaa atatcattta gggttgtttg
     8521 ttaccgggct tgaccaggaa actaactgga acacagcaaa agaacctgtt gggttctttc
     8581 atttaaaagg ctatgtgaac aacttattga ttaaagctgg cgtaaatatt gcccaggttg
     8641 tagcagaacc tgttaatcat cctcttattt caaatggttt acaatattca ataaatggaa
     8701 aacttctggc tgtattggga agcattaaat ctgctgtttt aaaaagcttt gatatcaagc
     8761 aggaagtgtt tgcagcagag gtagaatggg acttggtgtt tagcctggct aaaaagaaaa
     8821 aggtagaata taaagaattg cctaagttcc ctgaagtgaa gcgcgacctt tcgatgatac
     8881 ttgatatgca ggttacgtac gcacagattg aacaattggc ctataaaaca gagcgaaaat
     8941 tgttgaaaaa cgttaatttg tttgatgttt atcagggaga taaaatcgaa caaggtaaaa
     9001 agtcttatgc tctgagcttt atcttgcaag atgaagagaa aacccttaca gataaccaga
     9061 ttgacaaggt gatgcaaaac cttatcaagg cgtacgaatc gcagcttggg gcaaaagtaa
     9121 gaatgcagtt gtaatatggc tcagattaag gttattaagg acgatatcac acgccagcag
     9181 gtggacgtga tagttaatgc agccaactcc tctttgttag gtggcggtgg agttgatggc
     9241 gcaatacacc gggctgccgg gccagccctt tttgctgaat gtaaagctca taatggctgt
     9301 aaaactggcg atattaaagt tactaaaggt tatcagcttc cttgtaaata tgtttttcat
     9361 gctgttggtc caatttggca aggaggtgaa cataatgaag aagaactatt gaaatcttgt
     9421 tacttaaaaa gccttaaaca tgccaccgaa ttgaaagcaa aaagcattgc atttcctaat
     9481 atttcaactg gtgtgtatca ttttccaaaa gaacaagcag cagaagttgt tctaaaaacc
     9541 gttcaatgtt tttttcaaga acaaaaaagc tctttatcag aggtatattt tgtttgtttt
     9601 gatgacgaga attttgaaat atataaccgt ttacttccca aatatatcaa gtaatgtcag
     9661 taaatgaggt aagtaaatac ctttcgcttg ttaagtttag tcatactctt tttgccatgc
     9721 cttttgcttt tattgggtat tttttggctt taaaatatgg ggactcttat ttttcattaa
     9781 aattattatt gcttgttgtt gcatgtatgg tatttgcccg taatacagca atggcattca
     9841 atcgttatgc cgacaggcac attgatttgc acaatcctcg cacggcattg cgcgaaattc
     9901 ctgccgggat attaaaggct gaatcggtat tggtatttac tataataaat gcagtattgt
     9961 ttattgcaac tacctggttc atcaacccaa tggcttttta tctgtcgcca gtggccttgt
    10021 ttgtgattac cggatatagc ctgaccaagc gttttacagt actttgtcat tttatacttg
    10081 gattaggcct ttctttagcg ccaattgggg catacatagc tgtaactgcc cattttcatt
    10141 ggcttcctat attgttttca tttatagtcc tgacatgggt tagcggtttt gatatcatgt
    10201 acgctttaca ggacgagcgt tttgacaatg actggaagtt aaaatctatt cctgccaagc
    10261 tgggaagcag gcgtgcattg atattgtcca ttttgttaca tacgatttct gcttttctgg
    10321 tagttttagc tggtacatta gctcacatga acctattata ttggatagga gcgggggtat
    10381 ttatcacttt gctcatttat cagcacttga tagtaaagcc taatgatatc tcaaaggtaa
    10441 atcttgcttt tgctactacc aatggtattg caagtgttgt ttttgcagct tttgcaatag
    10501 cagatatgct ttttacctga caaacaccca tttgttctca tccgatactt ctggataaaa
    10561 ggaatagcca gcttggttga agtttttcaa atcatccggg tcgtcaattc tgtttttcat
    10621 aataaaactg gtcatcaacc cacgggcttt tttgacatac accactatgg tctttagctg
    10681 gtcgccagaa agttcataaa aaacaggggt aatgattttg caggaaagtt tgctggtgtc
    10741 caaaacactg aaatattcgt tagaggccag gttgatcagg gtatttattt ttttttcttt
    10801 catgtgtttt tcaagcgagg ctgtaacctt attttgccaa acttcataaa tgtttttata
    10861 tttttcaaca ctaaaagatg tccccatgtc cagacggtat ggcaaaataa ggtctaaagg
    10921 cctcactatg ccgtacaatc cggataatat acgtaagtgg tcttgtgcaa aaagcaaatc
    10981 ttgttcagta tatgattgta catttacacc cctgtaaact tctcctgtgt atgagaaaat
    11041 agcttgcaaa gcattgtttt ggttggtatc tgtgtgccaa ttgtgaatgc ggtcgaggtt
    11101 aagcctggcc aactgtggat tgatttgcat caacgactca atttgcggag cattcaattc
    11161 acgcagcgta tggttgattt tttttgcttc attcaaaaat tcgggttgag taaatgtggc
    11221 aaatttcttt tttgccttgt ctgtcattgt tttggcagaa gaaattacga ctaaaaagct
    11281 catgttgtta tattatttgt tttaatcatt aaacataaaa aggggataaa agtttagttt
    11341 atgaaaaaat aggtgttctg atgccttgtt ttcggctaac ttttttattt ttggtacgca
    11401 ataggatagt ggttggccaa aacttgtata tttggctgaa aataaggtat ttttatataa
    11461 agttaaaact gaacaaaagg cggagatgca aaaaatatta gctttcatag ctatttttgt
    11521 gatgttagta tcatgtaata caatcaattt ctccgatcaa ccacaggggg tcattgagta
    11581 tgaagtgata taccttacca acaaatctgg tatgcctacc aacttgttgc cccgcaaagt
    11641 tgttttaaaa tttcgttcaa acaaaagcat tactaccatt gaaggtttca tggggttgtt
    11701 ttctttaagt aatattactg atttcaggaa acataccaat tcaatcctgc taaaagttat
    11761 ggataacagg cttgtttatg aagggaaaaa aaatgagccc ccattctttt taagtaaata
    11821 tttcgattgt tctatagcta ttaccaatga aaaaaaggta ttggcaggtt tgccatgtta
    11881 taaggcaatt atttcggttc ctaataataa ggaaaaaaaa attgtctatt ttacccaggc
    11941 cataccatta aaaaatgcca acaaagcatc gccttttgct tctttagaag gaatattgat
    12001 ggagtttgac ctatcgctta ccaatataga aatgcacctt aaagcgacca aatacattca
    12061 aacagaggta gatgctgcta tattcaaagt tgatgacagc tataaagaag taagccagcg
    12121 aaaaattatc agcatcctga acaaattgct ggaatagtta tcaacaattt atttttccac
    12181 tgctttactt tgtcgaaaat actaaatttg gcaagtccaa aaaatgaatt tcatggctaa
    12241 agaaaaagac aaaaagccca aagaagacac caaagaagat aaaaaaggga tttttacaga
    12301 tatcaggttt aaagccattg caggtatcct gctcatactt ttctctgttt atttgaccgt
    12361 tgcatttgtt tccttcctgt ttacctggaa atatgatgcc agctttacct ggcaaaatgt
    12421 attttctggc tcagaaatag aagtagccaa ctgggctggt aaaaccggtg ccagaatagc
    12481 aaactggttg atgagcgagt ggatgggcta cgctgctttt ggcattccta ttattatgtt
    12541 tgtaggtggc acacggatgc tcggttttaa aaggtttaaa tttagaaaaa ctgcattggt
    12601 ggtgcttttt ggaatgataa tatcttcatt acttttcgga tatttttcaa gcataataga
    12661 atcttttagt aatggtacaa aaatatttgg cagcggtccg ggcggagctc atggttattt
    12721 tgtatcgcat tggttaaatg cgctgttggg taaggtaggt actggcttta tgttgctatt
    12781 aatagctttt acctatacag cactgatatt caagccattt gcacaatggt ttgtacaaac
    12841 agtgttggac atgttcaccc acaaacctaa ggaagtgcct gctgaagctg aaggtactgc
    12901 catgactgac ggtattgctg aaaatatgga cattccggct cctccggtaa tcgaaaatcc
    12961 cactgaagat attaccaaac aacttaacaa tgatgagctg gaagttattt tccaaaaacc
    13021 cggagatgag gatgttttaa gcacgccgga accagaagat tttgatttgg aagatgaaga
    13081 cgatacagaa cttgaattgg tagaaggggc agaggaagaa acaacagatg cagcttcgtt
    13141 gctccaggag cttggccctt acgaccccac gctcgacctt tccaattaca aatatcctac
    13201 tatcgaactg ttagaagacc ataaatcaaa caatcctgaa gtaactcagg acgagcttat
    13261 ttctaacaag aataagattg ttgaaacgct caaaaactat aagatagaaa tatcgcacat
    13321 caaagccacc attgggccta cggttacatt gtatgaaatt atcccggcac cgggcgtgcg
    13381 catttccaag attaagagcc ttgaaaacga tatagcgctt agccttgcgg ccttgggtat
    13441 ccgtatcata gcgccaattc cgggtcgtgg cacaataggt atagaagtgc ctaaccgtaa
    13501 ccccgaaacc gtgtccatgc gttcggtcgt ttctaacaaa aagtttcagg agtcgaatgc
    13561 tgatttagcc attgttttag gtaaaaccat ctcaaacgac acgtatgttg ttgatttggc
    13621 caaaatgccg cacttgctgg ttgccggggc tacaggtcag gggaagtctg tagggcttaa
    13681 tgccatcctt acctctttat tatataagaa acatccgagt gaacttaagt tggtacttat
    13741 agatcctaag aaagtagagt tgacattgta ttcgaaactt gaaaaacatt ttcttgccaa
    13801 gcttccagat accgaagagg ccattattac tgatacccag aaggtgataa atacgctaaa
    13861 ctcgttgacc attgagatgg aagaccgcta tagcttgctg aaaaaagccc atgtgaggac
    13921 aataaaagaa tataatacca aattcaaaga acgcagactc aatccagaac atggacatcg
    13981 atttttgcct tatattgttc tcatcatcga tgagtttgca gaccttatca tgacagcagg
    14041 ccgtgaaata gagaccccga tagcccgttt agcccaatta gcccgtgcta ttggtattca
    14101 tcttattatt gcaacacaaa ggcccactac taatattatt acagggatga taaaagctaa
    14161 cttccctgcc cgtattgcat ttagggtaat atcaatgatt gactcgcgca ctattcttga
    14221 cagtccggga gcaaaccaac tgattggacg cggcgatatg cttgtttcga caggtagtga
    14281 cctgataagg gtccagtgtg cttttgttga caccccagaa atagagaaaa tagttgattt
    14341 tattggaaac caaccaggat actcatcggc attcctctta ccagaggtta aagccgaagg
    14401 cgaagatggt agcatttcgt cagaagactt atcttcgcgc gacagtttgt tcgatgaggc
    14461 tgcccgcttg attgttatgc accaacaagg atcaacctca ctgatacaac ggaaattttc
    14521 tattggctat aaccgcgcag gccgtattat ggatcaatta gaagtggcag gtattgttgg
    14581 gccaagtgaa gggagtaaag cccgacaggt gcttgttgtg gacgaaatgt cacttgagca
    14641 tattttaaag cgtattgatg gaagaaacaa ttaagaatta aaaaattaag aattaaaaaa
    14701 ttaagaatta aaaaattaag aattaagtac gaattaagaa tgataagtaa gagatatatt
    14761 gtagctgcat tgtttatatt tattgcagtt tcttattttg atacaattaa agcccagaaa
    14821 gaccctgagg cgaaaaaaat tgtagaacag tttgtcagaa aaaataaggg acttgatgca
    14881 atatttatga aatttactat aaatacggag aaccatcaaa ataaggataa aacttcaatc
    14941 aatggtgaag ccctgctgaa aggggataaa tataagctca atatcaacaa aacagttata
    15001 tataatgatg gaaaactggt atatagtttt cttcctgacg aaaaagaagt ttctatttct
    15061 aattttaacc cgcagaagga agaaaatatg atggtaaacc ctgcctatct ttttaaaatt
    15121 tacgataaaa attataaata caggttattg ggggaaacaa cttttaatag caaaaattgt
    15181 tacgaaatag acttttatcc caacgacctg aataaaaaat attctattgt aaaactcata
    15241 atcagtaaag aaaaacttag tcttgaggca ttgaaagtaa aaatgaaatc tggcattgat
    15301 tatattatta aagtacagtc ttttgaggag aaaaaaaaca tttcggatag tgagtttgtt
    15361 tttgatacga aagcccaccc ggatgttgaa gttgttgatt tgcgcaagaa aaaataggcc
    15421 ttgctatgct aaaaaagttg accataaaga actatgtatt gattgatgat ttgtccatca
    15481 gttttgataa cggtttttcg actattaccg gcgaaacagg agcaggtaag tcaatattac
    15541 tgggagcttt gtcgttgcta gttggacagc gtgcagatac agatgttttg cttgataaaa
    15601 cttcgaagtg tattattgaa gctgtttttg atgttgaaca atatgctatt caatcttttt
    15661 ttcaacaaaa tgagatagat tatgatgtgc aaactattat acgtcgcgaa atattggaaa
    15721 cagggcgttc gcgtgcttac attaatgata caccggtcaa tctttcggtt ttaaaagagt
    15781 tgggcgataa actcattgat attcattcgc agcaccaaaa ctcgtatttg tctgaccagg
    15841 tttttcagct caaagttata gataccatgg cgcataatgc tgccctgctg agtgagtacc
    15901 gtgaacaatt cagaaaatac cacgaatgta agaaacaatt gatagagctt actgaaaaag
    15961 ttgaaaaaga caaagcagaa ttggggtttc tgcaatatca gtttgatgag cttgtaggtg
    16021 ccaatcttaa ggcaggagag caagaagaac ttgaaaacga agcaattgtt ttgtctaatg
    16081 ctgaaagtat aaaaatagcc ttactcaata tttcacatta tttgcaagct gaggagaatg
    16141 gtatagaagt acaaatgaaa gaagccctca atattgcaca aaaagtaaaa aacatatttc
    16201 ataaagccga aaacattgcc gaaaggttag ggtctatgta tgttgagcta aaagatttgt
    16261 cagccgaaac tgaacgttat gctgaacagg tagaacttga ccctgagcgg ttgacactgg
    16321 taaaagagcg tcttgatatg ttgtattcgt taagtcagaa acataaagta acttcaagtg
    16381 aagaattgat tgggttgcga gacaagatag gagagaggtt gtctaatatc cagtcttttg
    16441 atgagcattt ggctttgtta cataagcaaa ttgaggattt ttgtgcttcg cttcatgaaa
    16501 tatcactaaa acttagagaa aaacgtaagg ctatagcccc ttcgcttgaa aatactgtta
    16561 gccaaatgtt gcgacaactt ggtatgccaa atgctgtatt tgttgtgcag tttgacgagc
    16621 tgaatgattt tcagtcttca gggcttgatt cggtacagtt tctttttact gccaataaac
    16681 aaatccctgc ccaggatatc agcaaagttg cttcgggagg cgaaatatca cgtaccatgt
    16741 tggccgtaaa atctgctatt gcctcatctt tagctatgcc tgccattgta tttgatgaaa
    16801 tagatacggg tgtatcgggt gaaatagctg ataaaatggg gggtattatg caggctatgt
    16861 ctgccaatat gcaggttatc agcattaccc atttgcctca ggtggccgct aaaggccaat
    16921 atcagtataa agtatttaaa acagaaagct caaaggccac taacacccat atcaaaagac
    16981 ttaccaacga tgaacgtatt attgaaatag ctaaaatgct tagtggtaag gatgtttcgg
    17041 aagctgccat taccaatgcc aaagtattac tgcaatctta agtatcaata gattataaaa
    17101 tcttccaggt ttttttacca aaatatttgt aagataaaca aaacacacta tctttgcacc
    17161 gcattttaga aaatgtcatc agcatggctc cgtagcttaa ttgaatagag catctgacta
    17221 cggatcagaa ggttacaggt ttgagtcctg tcggggtcac taaaaataat accaattgta
    17281 agtcaatgat ttataattgg tatttttgtt atacagacat ctttaccttt aaaatttcaa
    17341 ttttcagttg ttccatttta tacacgaagt ttagcccatc taaatcatat cttttcaaaa
    17401 aaaagccttt taatgcttaa tttgggttaa actgtcataa tcaatacccg gtactatcat
    17461 gtatttttgt atttacattt gaaaagtata ttgtttaagc ttatgataaa atatatttat
    17521 attggtgttt ttctggttct gaacttagta caaatcgaag tttattcgca gcaggatttg
    17581 gctaatacaa aaaaaagctg gttatatacg tctggtattt ggttttcaaa aaccttcaac
    17641 aactttgata cggtatttgt aaaaaatacc cccagcaaat actcattgat tttgcgaaac
    17701 tctaactggc tgaattttta caatttcgat atggacaaca aaaacaagat tgtcatgcaa
    17761 tctgagttgt attacaatgc aggttttatt ttgggataca aattcctgtt attgggttat
    17821 tcggtcaatc tgaacgaaac tttttcaggc caaacggtta aaagccagga attggacctg
    17881 catgtaaact gtagctgcct gggtatagat gtatattata cagacaatca gggggcaaca
    17941 agcattaccg aatttaaggg tattaaaaat ataaaaaaca agggtgtttc gtttgatttg
    18001 atggcctgac catgagaacc ttggatggcg acataaatta ttatttcaac aataaaaagt
    18061 atgccaattc tgctgcatat tcaaggggct atttcaaaca gcagaagaaa agtgctggtt
    18121 catttattgc cgggctttct tatgccaggc accgaatata ttttgatttt accaatttgc
    18181 ctgaggatac aatgtattca tcgctaaaaa cgctggacga caagtctata ctttataata
    18241 gctattgcct gaactttggg tatggttata acctggtatt ttcaaagaaa tggctggcta
    18301 accttaccgt aatcccctct gccggcttta attcatacaa aaagaagatg agcgaggata
    18361 ttcccaataa taaaatttcg atgaatacaa aagttaaagc cgcgttggtt tacaatatgt
    18421 cagattattt cctgggattt tcggccatat tctctgccga ttggtttttt gcggataatt
    18481 atactctggt caattccttt ggaaatttca atatttctta tggaatgagg ttttgaaaag
    18541 ttaatacttg gaagcttaaa ccttccaggt tttgtaaacc tggaaggttt ttcaataaat
    18601 ttggctttat tcttcaagca agccgcgttt ttttagcata ggtttaatat cgggcttgcg
    18661 gccgctaaag tcataataca tttggttgta ttcctcagta ttgccgcgcg acaatatctt
    18721 gtcacgaaaa cgctggccgt tagccctggt cataccccca ttttctttga accattgata
    18781 tgcatcattg tcgagcattt cggcccacga ataggcgtaa tatcctgctg cgtagccatt
    18841 gccccaaata tgcaagaagt aacttgagcg ataacgaggt ggaacctgtt ccaaagcaag
    18901 attggtgcgt tttaaagcgt ttgtttcaaa ttcatctact gattttattg catctcctgc
    18961 cttaatggta tgccattcca tatccaggct ggcagcagcc aatatttcag taaaggcata
    19021 tccctggttg aatgtttgcg attttttgat tttttctacc aaatccttgg gcataggctt
    19081 gccagttttg caatggatag cataattgct gaataccttt ggttcaagtg cccaatgctc
    19141 gttaaattgc gaaggcagct caacaaaatc ccttgctaca cttgttcccg aaaggcttgg
    19201 ataggtttgg tcggcaaaca tgccatgcaa cgcatgccca aactcgtgga aaagtgtagt
    19261 gacatcgtca aagctaatca gagcaggctc gcccggagct ggtttggtaa aattgcaaac
    19321 gttgtaaatg gcaggtttgg tgccaagtaa ttttgattgt tcaacaacat tgctcatcca
    19381 tgctccgcct gatttattgt cgcgtttgaa atagtcgcaa taaaacaggg ccagctcgct
    19441 gccatcttta tcataaacgg tatatacccg catatcgcta ttatataccg gaatgtcatt
    19501 tctttgtttg aatgtaagtc catacaattg gttagcggca taaaaaacgc ctttttccaa
    19561 tactgtgtat agttcaaagt aaggctttaa ctgggtttca tccaggtcgt aacgctcttt
    19621 gcggattttt tcggcgtaat aatcccaatc ataaggttgt aacctgaaac ctcctttttc
    19681 tttatcaatc aatgcctgta agtcggctgc ttctttgcgg gctttagcag tggctgcagg
    19741 gacaagtttt ttcaaaaaat cgcgcacagc ttcaggggtc ttggccattt ggtcttgtaa
    19801 attccatgca gcataattgg ggaaacctaa tagttctgct ttttcagccc tgatttcagc
    19861 tattcgtaaa atagtttgac gggtatcatt tttatctccc ttttcagccc tgctccagga
    19921 agcttcaaac agctgttgac gggtttgcct gttttccagc gattgcaaaa gaggttgttg
    19981 ggtggtattt tgcaaaggga gcatccattg gccttttttg ccggccgctt cggcatcttg
    20041 cgaagcagag tttaaagtcc cttccgcaag ccctttcagc gtttctttat tgttggtaaa
    20101 gtaagcccct gcttgtgctg ccgctaaaag ctggttggta tatttagcgc ttaatccggc
    20161 ttcttcttcg ttcagttttt tcaatttctc tttatcctgc gatgaaaggt cagccccgga
    20221 ttgctcaaat ttgaaataat aaaactccaa caactttgca gattctatat ccaatttgag
    20281 ttcgtttttc ttttgataaa ttttttttac cctttcaaaa agcttatcat ttaaaaaaat
    20341 agcatcagtt tgtgctgcca acttaggcga cacctcttcg ttgagctttt gtaaaaccga
    20401 atcggtattg gcccctgcca acatattgaa tatggcattt accctgcgaa gcagctgtcc
    20461 gcttttttca agtgctacaa gggtattttc aaaagtaggc tgttcggggt tgttggcaat
    20521 tgagtcgatt tctgcccgtt gttctttaaa accttcgtca aaagccgggg caaaatcgct
    20581 gtttttgatt ttggtaaaat caggagcctg aaaaggcaag gtactgacat gcatcaaagg
    20641 gttggatgcc aagttaacag agtcttccat agttgttctg cgttctgagt ttgagcggca
    20701 tgcggccaat gatatcagca atacgccggt aagtaaaatg gatatatttt ttatcataac
    20761 aaaattattt aattattctt gtttcatttg aaccccaaaa ttattaaaca ccattgtctg
    20821 atataaattt aagtatttgg gttaaagccc aatgtattct tagctttgat taattccagc
    20881 cttaaagctg gagttacaaa ctcttacata ttgatatttt atcaaacaac agtatttatt
    20941 aaatattaaa gctcaaaata gcattggcat cgaatacttc taaattgata caataaaaca
    21001 cttcactcaa caaaaagttg aaaaagagca atatttactt tacatcaaat tcatgctttt
    21061 tataaaaagg tattccgtct tctgtaaaac ctgcaatatt cactacatac ctgcctttta
    21121 cattagtatt gtcaagtttt acaagggctt taccttctgc gtcaagcatg atgttggggc
    21181 accaaaatat tgtattgctt gttggtgatg ccttaaatgg ctgaacagcg gcaaattctt
    21241 tcactttgta ttcattgtta ataacgggct tattgctatt ccctttttta gtgataattt
    21301 ctactattcc attgggagca aatgagctgt accgcgatac ttctaccgga tcggtcaaaa
    21361 tacgaatttc ggcaatatca ctggaattaa ggtttttaac aacagtaata tcatccgatg
    21421 ttagtacgct gtcgataaca aacataacgc caatggtaaa tttaaaagac cttacagcat
    21481 tggcaaaaaa catcttgttt tcattgatgg tatatgattt cttttcggct atcaattcca
    21541 atacggtttt tgaagaattg tatttgtttt ggtcaatcgt gttgtacttg tttcgtttgg
    21601 cattcagcgt agggtcgtaa agcatatctt cactgtttgc ctcattgctg aaaatcgggt
    21661 aattgcgata cagatagtct gtcataaccg tatttttagc cgttgacttt atattttcgt
    21721 caaatttgct tttcagcaaa atgttgatag gaatatcttt tttgaacaag gtaggggtta
    21781 tggtataatc aactgtgcca accgactgat tgatatctgg acagataaaa taacctgagc
    21841 tgtctgtcgt tgctgtggca acttccaatg tttttttatt gagcaggctt aattgaagct
    21901 ttggtattgg tttttcgctg taattggtca caaaacctga tattccgtct ttaggcgaat
    21961 attttacttt ttttagggat gaatttaaaa aaggaaattg tggcaaagac gaaattaaaa
    22021 gcatgtcaat tgttgtattt tcttgcgaca acgaatcaat cttgtactcc gaaaaatcat
    22081 ttttcatcag gtaagtattg aaatttcgtg tatcatagcc caaaatgttt tcgttttgct
    22141 tataaacaga tatgctgaag ctccctttta ctggtgtacc cttgttatcg ttgactttta
    22201 tgctaaaagc tgtgttattg tcctctggtt ttattgaagc gataatcgtc cggttatggt
    22261 ctatatatgt actgagcgag cttatgactt cttttttgtt gttgaaaatt gtcacatgca
    22321 ccaccccggc agggacatcg tttacgggaa ccctgaacat gccggctttt gaattaccga
    22381 attttgaagc ccaatatatt ttcccggcct tataagcaat gcaatacaaa tcagacgctt
    22441 cggtgttatt gacattttct aacctgatca tcaacgtgtc ttttgtcttt tttatcagcg
    22501 acatggaagt gcctgatgtt tttacaggca aaatgtattc tgaagaattg ccctgagggc
    22561 ttgttaatgt ggcttttagc gttgaattgt cttttgaggc tacaatcaca ctgcccaacc
    22621 cgtttttgtc tgttttaaaa actgtgctaa atgggccttt tgaatctttt atttcgcctg
    22681 atactgctac atagttccca tattcatcgg tagattttat aaccacttgc ttggcaaatt
    22741 ccgagagtat ttttcctcct tcgggaaaaa acgaaagcct gatttttgaa ggtgaaggat
    22801 atattacagt gttggcagtg ctttcatttc ctttgtattt cgacgtacat tttataaaaa
    22861 taggagtagt aaaggattct gcaggaatgg taaatacaaa cttgccggaa gcatctgttt
    22921 tttgggtttc tttatagctt tttttgttgt ctgcaaaaag ttcgagcttg acttttgcat
    22981 cggcaacagg ctgcaaatca ttgctgctga cagtaacttc aagttttata ggctcttttg
    23041 ctgaaaagta ttcattgatt ttttttatag aaatactgta atttgatgct ttgttgtttc
    23101 tgatttcaat aaattttcta aaaatatggt tcagcgagtc gttttccatc caggtagaat
    23161 gggccaaaat ggtataatac ccgtcagata actttttggg cagtgcaata ttgcccacag
    23221 ctaaactgct gtcaacccgg aacttttcat cagccacaac tgtgccttta ttatcaaata
    23281 atttaacttt caatcctttg cttaaacccg aaggcatgtt atcagaaacc acctgtgccc
    23341 taaaccaaat attttccgaa ggcttgtaaa cctctttgtc tgtggtaaaa tagatgtttt
    23401 cttcagggta ctcctggtga taggttttta ttttttcggc caggtcattt ataacctcat
    23461 cctgggcagt aatgtttgtt gtataacatg ctaaaagcat gatggcaact gggaaaattt
    23521 tatatttcat tttttatagg tattgttgct tttgatattt attactataa caaaaaaaga
    23581 ataaagaacc cttatcctta tttaataaga agttaaaaat agtcattgtt gtccgattaa
    23641 aaaaattgcg ttcctgcggg acttttaaaa gaagcagttt attttatttc taccaatatt
    23701 ttgcccgccg tggcgcatag ccgtcaggct aagaaatttt gcatacgaat tctattttga
    23761 agccctgtaa gggcgacata ttggtagaaa aacaagttat taaaaagaga acgcaccaag
    23821 cataaacaaa atacaacgaa caaatgctga atggtgcgta atttggtgaa gctttttaaa
    23881 tgcagttctt ctattgcgcg cccaaaagag tttcgttcat tttcaaacat tcatctgaca
    23941 cgctttttat caatactttc tttttctacc aatatgccgc ccatactggg cttgtcacag
    24001 aagtgcgcaa gcgggagtct gtgtaaaaag tattgatttt tctaatttac ccctaaatcc
    24061 cctgaaggcg acttttgaca atcaataact taggacaaag cccctttaga gctagtctcg
    24121 tctcaggcgg gaggtttggg gtttaaattg actttctgcc aagcgtacct ttccaaacaa
    24181 caatgatttt atttaacaat ataaaaagtc aaaaagttaa ccgcaaagaa cgcaaagtgt
    24241 ttataatcaa tatttaaact ttgtgttctt ttattctttt ttcgtgaact ttgcggttaa
    24301 ttacttcttt ttacacaact tcttttattt gattgtactg ttaatctatc ttgagacatg
    24361 tgtcgctcct acagagctta ttttaattat aatgcaacac ttcaccatac tattgcccct
    24421 acggggctta tcattagctg cgttagcagc gatagtatgg tagtatagtt gtatcaaaga
    24481 tattaatagc tctgaaggag cgatacattt ttattactgg tttaatacca gaaaataata
    24541 tgtaaaacat tttgcgaacc tcaataaaaa aagctgccga attgctccga cagctttttt
    24601 tgaaattttt aattcgtaat tgacacttga attacgcttt tttacttctt ctttcgttca
    24661 ggtcatcttc aatttgctca agtttctttt cgttgagcgg atagatgaac atgaataccg
    24721 cagcaagtat agcacctata gcaggccaaa tactgagcat catacggata ccgttttgtg
    24781 tttcggctgt ttgctctacg ttagcctggt agccataaat agcaagtaac catccggcaa
    24841 gtgcgatacc tattgaagct ccaatctttt gtgacataga agctgcagag aataccagac
    24901 ctgttgcacg acggccattt ttccattcgg agtagtcggc tgtatctgca tacattgacc
    24961 ataccaatgg gaagatgata ccagcgttaa tactgatgag tacttgtaaa acaaggatca
    25021 atgtaaggtt gcttctgtcc acccagaaga agatgatact caacacagcg gcaaataaca
    25081 tagcgctaag gaaggtgctc tttttaccaa tcttgtccga taccggtttg gcaaggataa
    25141 ccccgacaat gttggcagct tgtcccaata gcaggtaaat agtgcttatg gcaagggtca
    25201 tattgaacga atcgtaggta atatcgcttg ctcctttgaa gtaatatttg aaatagtaaa
    25261 cagcagcgcc atcgcggata gtattgaaga agatggtcga aatacctgcc cccagaagga
    25321 taaaccaagg gatgtttttg ccaaggtctt tgaggtcgtc tttcagcgaa gttttttctt
    25381 ccaaaggttt gatgcgttct ttggtcataa ggaaagtcaa caggaagaag ataaccgcaa
    25441 taatagcaat gatgataaca gctttttggg taccactgcg atagtcaatt tgtttgatgg
    25501 caatcttttc cagtttcaaa tcagcttcgc cggcagtagt tacaagtact tgccattgtg
    25561 ctgaatccga gaaactatcg agtcctttaa aactgctgag ttttagttca atattgctcc
    25621 aggccccgtt acgcattagg cccagagtgt ctttaccatc tgcaaaagta aagcaaccgg
    25681 tgtcttttgt agattggttg attacacaaa ttttaattgg ctcagcactc ttgatactgg
    25741 cttttgcagt tacatatagc agtgagtcca caactgtttc gtcttgctct ttttgagttg
    25801 tgccgctcca aataaaattt ttttcaatca ctttggtttg ggtagaatct ttaacctcaa
    25861 taacattgcc attgctgaat ttgccaatgg tgtcggtccc aaaaccattg aaaagaggtt
    25921 ggaacagacc aagtacaagg aaactgccgc caaaagcaaa gataaacctg aaggtagcaa
    25981 gggttgtacg ttctttacca tcggcagtca taacgcccat cagcgaagca taaggaacgt
    26041 taatcataga atagataagc atcatcaacg aataggtgat gtacgcataa acaattttgc
    26101 ctgtaacatc aaagttaggt gtagtaaagg tcatcacacc gatgatccca aaaggcaagg
    26161 caaaccaaag caggtatggc ctgaactttc cccatcgggt ttcggtgcgg tcgcagacga
    26221 tacctacaaa cgggtcaaaa aatgcatccc cgatacgtgt tattaaaaac attgtcccaa
    26281 ccactgcggc cgagatgcca aaaatgtcgg tatagaaaaa cataatgtac atcccgaaca
    26341 acttccaaaa catggacgat gcggcatctc caaatccata cccgattttt tccgatagtt
    26401 tcaatttttg tttaaacata agtgagtgta ttaattaatt tagtttttta tcgaataaat
    26461 aggcttatca agccatgcgt aattccattt atctgaaaag taagtaaaaa taagcgttgt
    26521 agttatacct ataatagaac ctgccgttac atctatcaaa aaatgttgcg aaaggtacat
    26581 ccttgaatat gccactatac aagccaacaa caaccaaatt acctgggtta tttttttgtt
    26641 ccaggcaaat gccagacata aagataatgc aaaaattgat gctgtatgcc ctgaggggaa
    26701 actatgtaca gtggccatct ttaccccttc tacaaggtgc aggtgggctg ttccttcaaa
    26761 aaaagcaatc ggccttgggg catctatcag gcgtttgatt atttgcgctg ccaaacctga
    26821 tataatatag gtactaataa tgtttactga aactcttgtc cgataaagta aaaacaatac
    26881 gcctataata actacaaaca aaccatcccc aatgttggtg atggttttga aaaaatggtc
    26941 agctatatta ttgttcaatg aattgataaa caggtgtata tctgctttgg aaaagattgc
    27001 catgataatg aaacaggcca gtatcacgac aaagtaaagt attaaaaaca gctgggcctt
    27061 cttcactttt attgcattgt taatttctgt tcgctaataa aaacaatgaa aatgttatag
    27121 aaacactatt gcattatttt ttaaataatt ttgcacgtga aaatagttat attttttgtt
    27181 tcataaaatt taaagtgatg aaatttgaag gtaagtggga gaaacgatgg cacccgcttt
    27241 tgcaggaatg ggtaatactg gctgctacaa ccagcgaaag gccttggtct ggcgatacag
    27301 ttaagaatat acaagccgaa gaaccggcct ttgaccctaa atgttatttg tgccccggtg
    27361 ttacaagggc ttctggtgtg accaatgata attatacaga gccttgggct tttactaacg
    27421 attttgcttc gttctcgctt gatgcccctg atgttcaccg caagtcaaag tttgaaacag
    27481 tagagcctgt gcatggtaca aaccgtgtta tttgctactc gccggttcac aacgtaacct
    27541 tggccgaaat gcctgaagag caggtggcaa aagtagttgc gctgtggcaa aaagaatatg
    27601 ctacgttgtc tgtcaatcct aagattgaga acgtgttgat atttgaaaac aaaggaagag
    27661 caataggtgt atcaaacccg cacccgcatg ggcagatata tgctaccaat ttcgttcccc
    27721 gtattgttga aagacaacgc cagtcttttg ccgattatta tcgtgaaaat aaaagccact
    27781 tgctggttga ccttttggca gatgagcttt cgcacaaaaa acgtgttgtt tacgaaaatg
    27841 accactttgt agcttttatt cccttctttg cccgttttgt atatgaaact tatgttgttc
    27901 cgcgcagaca ggttgcccgc attaccgatt tgaacgaagc tgaaagcaaa tcgttggcca
    27961 atatgcacaa agtgatgctg agcaagttcg acaacctata taatatgtcg ttcccaaaca
    28021 ttactatgct tcaaaatgcc ccttgcaacg ggcatcccga caacgagttg tttcatttcc
    28081 acatagaatt ttacccacct ttgcgttcgc cggataaact caagtacctt gcaggttttg
    28141 agtcgggtgg gggaaatatc atcaatcctg tgctgcctga tgaagccgcc ttagctttga
    28201 gaaacgcgaa atattagatt tatataaatg aaattggctc ttaactgata gtgcgagcct
    28261 gtcttatttt acctcaaaga ttttatcgat gtttaatttt actacaaaca gggcgccgct
    28321 acgcgactaa atgagagctg cgtagctgcg aactgtttgc aggataaccc aatagttggt
    28381 tgttgcgcca atcatcctca aagcgcttca ggtctttcat aactggggcg ttgcataaat
    28441 ttaactgctt aatttcttat atttagatag gaaacacgct tgccaggcag tttagaaatc
    28501 ttgtttttgt tgtattttat gcggcctttg ccctgattat tgccataaca aacgccagca
    28561 aaacaaagca ggtgaggacg ataaaactca ggtgcagcga atgaagctct gcaataaaac
    28621 caagaagtac cgggcctgtc ataaaaccaa tattggcagt ggctgcaata aacgaaaccc
    28681 cgtctgccgt tttcactcct ttgattgtcg aggcaaggcg gtatatttcg ggtacaataa
    28741 tcgaaaatcc taaccctata ataaaaaatc ctataaaagc agtaacagaa aacgttagca
    28801 acactaaagt aaagcctgcc aggcttgtgc caatagccaa tcgcagtagt ttccatgaac
    28861 caatgcggtt gctcaatgca tcgccattaa aacgcccaat ggtcatacct actgcaaaca
    28921 aggcatatcc aaggccgata tactggactt ttacctgtgt tattttcttt aagtataagg
    28981 cactccagtc ggcaatagcc ccttccgata ccatcatgat taaaccgatg gaagctattg
    29041 ctaacaatgg cttaaaagga aattttttgt acttttcttt ttcaacagcc tcggtgcgaa
    29101 tatcataata ctcctttctt aaccaaatat ggacacttaa aagaacaact acaagcaatg
    29161 atacatgcag caggggcatt tttagtaaac ctgccaaaaa actgcctgtt gaagccccaa
    29221 tgataccccc tatgctccaa aagccatgac taccggtcat gatatattta ccagcacgtt
    29281 tctcgatagt agcagtaagt gaattaagcg atatagcaaa agcacaacta caaaatccca
    29341 gacaaaacaa agcagcgcag agcatggcat agtttttggc cagaaatata tttatcaaag
    29401 cgaaggtata taaaataaaa gcataaaagg cataacgacc aacccctatt ttgtctacca
    29461 gcctgttggt aatgggtatc ataaaaaaag aacccagcga agaaaaaaag atggccccgc
    29521 ctatcttgcc ttctgtaatg tttatttttt ctgcaatata agggatatag gttacccagg
    29581 tgctgaaaat aaggctaaaa caagtgtata aaaacgccgg ggcaaagaat ttccggcttt
    29641 gaaagaaaat tttaattgaa agcatatcta ttttgaaaac cgcgacaaaa ctaatatttt
    29701 ttgggcttta tggtatcaaa aagttgttaa acactttagc cgttaacata aatcctttcc
    29761 tactaaccat tgtttatttt atctttacag cttttaaata gataacaaaa caatcattat
    29821 cagatatttg tattgtcaat ggggctaagg ctgaaatatt ttctattcct gctgttattt
    29881 ggggcgatag gggttacgct tgtggcccaa aataagtttt tgcagccaga acttgtcccc
    29941 attaaaataa aaaaaggttt tacccttttt accctcgatc agagcattag cgctgccaat
    30001 gatactgtaa tatttctacc tgttaatcag gatttttata taaccaaaac ttcgtctatt
    30061 atcgccacca attttttctt gtcattaaaa caaaaggctt ttaaaagaag gtggtcgaaa
    30121 agtattaata atgttgttat ttctaccccc gacagtatcg ggcctgatga tacaattaag
    30181 gttcaaaagg ctgaaatacc ttttatcaat tatcaaaacc ttattatcag ggatatcaat
    30241 gtgagtaaac ttaatatttt tggcccgtca tttaacaatc ccaacgatat accccgcaat
    30301 gtttttgaaa gatgggtaaa ccgtacccat gtaacaacaa gagaatattt tatcaagcaa
    30361 tatcttttat ttaaaaaaaa ttcgaggctc gacccatacc agttggccga aagcgaacgc
    30421 ctccttcgcg agttgccttt tattgaagat gcccgtgttt atatttatcc tacctcgtcg
    30481 ccagatagtg ttgatgttga agtggttacc aaagatacct ggtctttggg ttttgacttc
    30541 accacggcca acataaaaga ttacaaactg gatgtttgga accaaaacct tatgggtacg
    30601 ggtagtgagc tggataatgt atatttcaaa aattcaaaaa aaatacctgt tgatggatta
    30661 aaaggcagtt atactatacg taacatatca gggtctttta taaatacatt gataggttat
    30721 gaaaaatttg gcaatgaaag tttccggatt aatttctggc gcgacttttt cacccagcaa
    30781 acccgctatg ccggacaggt ttactttgaa aatatcgaaa aaagatatgt aagcgttgaa
    30841 caagataccg gaggttatgt attttttccg cttaaaggga ccgtatcgag gttgtggctt
    30901 ggcagggcat ttaaattgcc tacgccttta atcagcgcta ctacaaaaac cagcttttct
    30961 attgccgggg gattgtacca gtacgattat accaaagcgc ctttggtaag agaaagatat
    31021 cgttacgaat ttcaaaaccg tactttttac ctgctttcgt attcattggc cagctatggt
    31081 tattatcgtt cgtctttggt atataatttt ggccgaacag aagacattcc ttatggcttg
    31141 ctcatcaaaa caactcaggg cctcgaggtt ggcgaatttg gcaaccgcta ctatcatggg
    31201 gtctcttttc tcaatggtgg cccgttattg tcattagggt tcttttattt tgatgctgct
    31261 gttggcggtt ttatcaaaaa aaaggaggtt gagcaggggg tattaaatgt aggtggcaac
    31321 tatatttcca acttgttggt tattgggcgt atcaaggccc gtgcatttat caatgtcgat
    31381 tttatcaaag gttttaaaag gtatgaggat gaatatcttg atatcaataa tttttcaggg
    31441 gtacgtggtt tttataatga ctcggcccgc ggtggacaac gtttggtgat aaatatggag
    31501 tctgtgtttt ttactccctg gtatgccggc gggtttaggt ttgccccttt tgcttttgcc
    31561 gattttgctt atgttggctc aaccaggcag tggatttttc gcaataatct ttatagcggc
    31621 tttggtattg ggctgcgttt gcgcaatgag cattttgtgt ttaaaacttt tcaaataaaa
    31681 ttggcttatt acccgttttt gagcaaaaaa gccagtggcg acttgttcac tttatcgcag
    31741 gaatggcgct tcaggcctaa tgattttact gtaaggtcgc ccgaaatttt gatttacaaa
    31801 taattaaaaa tataaaccaa cccttgccac cggatttgaa tatggtgaac gcttatcttc
    31861 taaaatattg aacataaccg atatgttaaa agaagaatgc ttgccaatgc tttgttttat
    31921 accgccccct actaatactc catgaagcca atagcggttt gtttttgaaa gcttctcggc
    31981 atccgaaaaa tcgcggtcta agttgatggc ttcatactca acctgcccaa aaaagcccat
    32041 attggtttta tatcgcgtgc ttttgcctat ttcatcaata aatgtatatt ggccatatac
    32101 acgtgcaccg tatatactcg agctgatact ctttgaatac catttttcat aataatactc
    32161 ataagttgct cctatgccgg ctattatttt gggcgtaata taatagccgg ccatgggcga
    32221 aagtcctatg tatgtagcac ttccaaattg taacccgaaa aatcccccga aaataaagtt
    32281 ttctgcaaac ttcgattttg ttttttccac tttcgtggtg tcttttgtag tctgtccaaa
    32341 aagacttgcc gaaaacagtg ctgacaggat gaatattttg attttcatgg agtttgctat
    32401 tagcttttat gatacaaatg tatttatttt ggctataatt ggaaacagat ttaaataaca
    32461 tttcgggttg ttttgctggt aaaatatttg gtatgaatat agttataact ggttgtagca
    32521 gtggtatagg ttttgaaact gtcctggagc tgctgaaaaa taaagataac aaggtgttgg
    32581 gcatttcgcg caataaagaa aaactgtctt tattggaagg tgctgtcaat tcagcacaat
    32641 tgcaaacgct ttcgcttgat ataacacagt tggacgagca tcagggtact cttcaacaga
    32701 tgctgcataa gcatttgcaa tcggtagatg tgttaatcaa caatgccggg ctcttggtca
    32761 ataaatattt tgaatctttt gaaccaatgg agattgacaa tattatcgat accaatttta
    32821 aagctcctgc ggccattatt aaatttttgc tgccctagct tgagaaatca aacaatgccc
    32881 acattgtaaa cattagcagt atgggcgggt ttcagggaag cagcaaatac cccggattat
    32941 catggtatag cgctgcaaag gctgctttgg ccgtattgtc cgaatgtctt gctgttgaat
    33001 tggccgaaaa aaatataaag gtaaattgtc tggctttggg tgccgtcaat actgatatgt
    33061 tgcaaaaagc tttccctgga tataaggctc ccattgaggc tgaccagatg gcaaagttta
    33121 ttgccgattt tgctttgaat ggtcacaatt ttttcaatgg ccaaattctg cctgtcaggc
    33181 tttcaaatcc ttagtaaatc tcttattggg ctagttgaaa taaaaattgc acccatttgt
    33241 agcgcgtact gtgctatgcc cttgcccata atcaatattc aagaaaattt aaaaatgttt
    33301 tttcgtctat tacatctata ttgagttctt tggctttctg gagctttgat ggccccatat
    33361 tttcgcctgc tacaataaat gatgtttttg aagttaccga atctacaagt tttcctccgt
    33421 tggcaataac catttgttca agttctttgc gccgttgcgg gctgctaaaa ctaccactta
    33481 ccactattga tttgcctttg agcttttcgg ataccacttt attggtatct tcttttattt
    33541 gcatctggac gcctgcctct ctgagccttt caacaataat ggcattattg ggtactgaaa
    33601 accatgctaa cacactttgg gccattacct cgccaatatc gtccactgct gtcaactgtt
    33661 caatggtcga atttttaata gcatcaatgt ttttaaaatg ttgggcaagc ttacgtgctg
    33721 ctgtttggcc aatatgtctt atccccaaag caaacaatac cctttcaaag ggggtttgct
    33781 ttgatgcttc tatgttttgt aagatattat tgactgtttt atcctgaaat ctcactatac
    33841 gtggtttact tccgtcttct gcattgtatg ttttttcaag gcctataatt tgcccgtatt
    33901 ttatgtaata aaggtcggct acatttttaa taataccttt ttcataaagg gtgtctatcc
    33961 ggccttcgcc taaggtttca atattcatgg ctttgcggct gataaagtgt tctattttcc
    34021 cttttatctg cggagggcat gagtcggcat tggggcaaaa gtgtttggct tcgccttctt
    34081 cgcgcacaag ctgtgcattg cattcagggc agcggctgat aaatactaca ggcttggcat
    34141 cggatgcgcg cgaatgtttt tcaacacctg ttaccttcgg aattatttct cctccttttt
    34201 caatacaaac aatatcgtgc tcgtgaagat cgagaagttg gatctggtct gcgttgtgca
    34261 atgaggcccg cctgacggtc gaacctgaca actgaacagg ttttaagttg gctaccgggg
    34321 taatggcccc tgtacgccct acctggtatg aaactgaaag caactcggtt gttacctgct
    34381 cggccttaaa cttgtacgaa atagcccaac gtggtatttt ggccgtaaag ccaagctctg
    34441 attgttgtgc caacgaatct actttgatta ctataccgtc aatgtcaaat ggcagcgaat
    34501 cccgcttttg gtcccaataa ttgatatatg ccaatacgtc ttttatggtg tcgcattttt
    34561 tgctgtaagg tgatatttta aatccccagc ctgctgccat ctggaggctt tggtaatggc
    34621 tgtccgaaat tggaacaggt gtgtaaaggt tgtataaata acagtcaagc tttcgtttgg
    34681 ccacttcggc agattttaaa agctttaccg aacccgcagc agcgttgcgc gggttggcaa
    34741 agggctcatc ggcggcttcc ctgcgctctt cgtttaatgc ttcaaaaacg gcacgaggca
    34801 taaatatttc gcccctgatt tcaaaatttg caggggctgt ggcgctggca atttttaacg
    34861 ggatagaacg tattgttttt acgttattgg taacaatgtc gccttttacg ccgtctccac
    34921 gggttacggc ataagccagt tgacggtttt cgtatattaa gcttattgaa ataccatcaa
    34981 atttcagttc tgcgctatac gaatattgtt gccccccgag gcctttgccc actcttttgt
    35041 caaagtcgag caattcggct tcgttgtatg tattgtccaa cgaaagcata gggtattggt
    35101 gctctttttg ttcaaattca ttgctaatgt cgctcccaac atgcacagta ggagaggtcg
    35161 ggtcaaaaaa ctcagggttt tctgcttcca gctgttgaag ttgtttcatc agttggtcaa
    35221 attcctggtc gccaattaca ggctggtgta aaacgtaata cctgtggtta tgttcccgaa
    35281 gggtttttct gagtttttct atttcgaggg ctatttgttc tttactcatt gaaatgtcct
    35341 tttttgataa taaaaaatgc cattcccgta acagaatggc ataaatactt gtagtttgtt
    35401 attattagta tgcttcaaac tcaaaaggga tttttaaggt aaactggtaa tgttcgccaa
    35461 gctcttgtac cgattcgtcg tcttcttcat aataccattt tacaaggcac tcattccctg
    35521 atatgtgttg ttcttttatc aacctgaaca tttcgagcaa tgacttggac gaaccgctat
    35581 tgatgtattc aaatttgagt tcaaaaattg tttttttctg tgggctcttg aaatagtcaa
    35641 ttaaccataa aaccaatggt ttgtaaaagt cgccggggtt ttcgggtatt gaccttcctt
    35701 ccaaacgcat taccccactt tgcgggtcaa aagtaacttt gggagtgttt gatgtttttt
    35761 ctatttgcag cgttgtcata ccaaatacat ttgtttatac aaatatatat aaaaatatca
    35821 attgttattc atgccgtaaa gatattatag ggtcaacttt tgcagctttt tgggccggta
    35881 cgtatcccga aattacccct actacaaaac ataacagtac ccctaacaat atccacaccc
    35941 acgggatgat aaacgatcct ccggtcaaca tggccaccat gttgcctata agtatcccaa
    36001 gtattatacc taatgccccg cccatttggc ctattataac agattcaagc aaaaattgtt
    36061 gtttaatggt gctggttttg gcccctaatg ctttacgtat ccctatttct gatgtccgtt
    36121 cggttaccga tacaagcata atgttcatca atcctatggc ggccccaaaa agcgtgataa
    36181 taccaataat ggtagcggcc atagttacca gcctgaggtt gtttattagc atattggcaa
    36241 ggctgttgct cgattctatc tcaaagtcat tagcgtcgtg cggggccaat cttctgacaa
    36301 tcctgaatat cccttcggct tcgcttatgg caacatcaag cagctctggt ttggccggca
    36361 atatgtttat acggtatgtt tgatcagggt tgggataata ctggcgggca ttggttatgg
    36421 gtatcaggca cagcctgtcg ctgccgttaa aacttgtccc ttgttgggct agtaccccta
    36481 taatacggta ttttccattg ccaacactaa tggtttggtt gatcgggttt tcattttttt
    36541 taaaaagttt ttttactaca tcggagccta ctatgataac gtgtttgccg ttttctattt
    36601 cttcatggct gaaatttcgg ccttgcccga tgcttttgcc cgatgttatc atatcgtact
    36661 catctacgcc ccatatttct atgttggggt cggttttttg catttgaaac ttgattgttg
    36721 atgccccgct aacatacccc gatatggcta ccgtggccgg gaactgaaaa cgtccaacaa
    36781 attctgtggc ctggtcgtag gtaatgttgg ccgtattgac cgtgcgctcc ctatggccgc
    36841 catggtgcat gttgttccct tttttcacaa tggcaaatgc gcttgccccc atggtcgaaa
    36901 attgggtggt aagtgtactt tttatcgact ctatggcggt caatatccca acaagggcca
    36961 taatcccaaa ggctataata aacatggtca gtatggccct cagcatgttg gcttttatcg
    37021 accttatggc tattcgtgtg ttttcgttca ggataccaaa attcatcagg cttgtgtttt
    37081 aattttttat caaatgtata aaaaaaagcc catccggggt ttttattttt tgcccgggtc
    37141 atatcattta cctggctctt gggctcctgt ttcaaatgtc aattttggca aactgtttac
    37201 tatatctacc gtttgcacgc tcacgttgat gatgctcagc aacaaatcta aaatgtaccg
    37261 tgggttacct gtttcggctg cccagtcgtt ggggtcgttt ttaatgccac tctccttgtg
    37321 gacagttact tggtagcgtt ccattaccca ctcaatggcg cttttgccgt ttaccacgta
    37381 ttcgtaagcc ttttcaggaa tattactcac ggttatgtgg ctgttgtata agattgtgtc
    37441 tttttggtct ttttttggga aacgcatttt ttcgaccgtg taggtttggc catcgtcccc
    37501 actgaccgtt acatgcggat aaggggcaac tgtttcatag tttaggtgca gttcggccag
    37561 ctggcggcct gccttgctaa atgcccaaaa gtctgctaca ctctctacca gtggcaggcg
    37621 gggcagcatt tttttcaggt cggcggcaaa ggtttctcta tactctttgc agtgcaaaaa
    37681 gccgtacacg taataaaata tatcttcttt actgacgctt ttgccgtatt gcgcccgggc
    37741 cctttctaaa ataaaatcgc ttacggcatc gcgccgtaca tacttgcccg cagcagtgcc
    37801 ccccgcagca gcttcgccgc tatcaaacaa gctcttttgt actactttgt tttcttcgta
    37861 gtaatagagg gggaagcatt gtccatttgc ttgtaattgc aaatcaggaa caacatctgt
    37921 tattaatgct gaaaaatctt tatttgtacc tgtgccacac aatgaaatga caatgttttt
    37981 atgattctta gttggaaata gttttggaag ctggtatctt tcttgattta agtcatcatc
    38041 aaaatatgaa tttaattttg tgaaaggtct gtgcattgtt attcgatact catattcgtt
    38101 ttcaattatt tcttttcttc ttttaatttg attgtcccaa tctcttgtcc agtttccttt
    38161 agttacatct tttataattt ctttattttt ttgttttaag attagatcta attgctcatt
    38221 ataataagca atgctctttt ttacgttttc acacaaagtg gcttttgaaa aattatatac
    38281 ccatgcatct ctgtttgtcg cagttcctaa tgtctgagct ataaaaacac tctgactttt
    38341 cgcatcaaac tttttttccg gttccaaagg gataaactcg tcaaaaaggc tgttgcgcac
    38401 gcttatccag tcgccatgcc cgttgggctg cagggttttc agcggaagtt tcggattggc
    38461 aaaggttttg aagcctttga ctattttcag cttttgttcg cggcccaggt agtcgcctat
    38521 gtcgtggtaa taaatggtgg ctttgtctgt ttttatatca gggtttttta ccagcagggt
    38581 aatggctatg ggcgtgcggc tgccgctgcc aaaaattttt ccgccttctt tgcggctcag
    38641 ctctccgctg gtacgttggt ttcctctcag gttaaacacg taaacggatg aaaactcttt
    38701 ctcgatggct ttgcggaagc catcggtgct gttgccgtcg agccaggcgc cgttgctcac
    38761 aaagcatatc acgccgccgg ttttgtccaa tcggtcggtg ctccaccgga aagcttttat
    38821 gtaagcatcg taaagcgatt tgttgaggcc tgctgttgat agcttggcgt aggtatcggc
    38881 aatgcgttgg tcgagccttg ggtaggcttg gttttgcgcg ttgtcgttag ccgatttttg
    38941 cccgatggag tagggcgggt tgccaataat cacgcgcagg ggtgcttttt gctggcgttg
    39001 cacgcgttcg gagttgcggg ggaacatttc cgaaaagagt ttgtcggtgt cgtcggtttc
    39061 gcccagctga aaggtatcgg taagcacaat gccctcgaaa ggggtgtaac gggctttgcc
    39121 tacgccatcg tcggggtcgg gggtagcatc gtggtaagcg ttttcgatgt tgatggcggc
    39181 aatgtaatag gccagcagca caatttcgtt ggcgtgcatt tcgtggcggt atttgcgttc
    39241 gaggtctttg aggccaatca atccgctttg cagcaaacgc accataaacg ttcctgtccc
    39301 ggtaaagggg tcgagtatgt ggatgttgtc gtccgaaagc gtgcgcccaa attctttttg
    39361 cagtatgtcg ttgacggaat ggataataaa atcgactacc tccacggggg tataaacaat
    39421 gcccagccgt tcgaccattt tcgggaaagc ggttttgaaa aacttatcgt acagctcgat
    39481 aataatgcgt tgtttgcctt cggcattgtc aatgccctgg gcacgtttgc gcaccgagcc
    39541 gtagaatttt tgcagggttt cgtcctgctc tgccaggttg ttgccttttt cgagggcttc
    39601 tatcatgctt tgcatggcca ccgaaacggc atttgatttt acaaacgaat aaccctcgaa
    39661 cagggcatcg aaaataggct gggtaataat gtgctgtgcc agcatttcaa tggcttgctc
    39721 ctggttgatg cctgggttga tgtttttttg caagccactt aaaaacttat cgaaggcctc
    39781 gcgttggtct ttcttttcgg taatgaggta tgcaatgcgt tcaatctggc gttcggcaat
    39841 cagggccacg tcttttgccc attgctccca ataacggcgg tcgcctactt tttgcactaa
    39901 gcgggcaaac accacatctt gcaactgctc aaactgaatg gcaatttgcc gccctatttc
    39961 tttgtttgtt tcgctgcttt gcttttcgcc ttgtcttttg tcaatggggg tgccatcgtt
    40021 atcaaacgaa gtatcggcgc cgccaacaat gatttggccg gggcgttttt tgttgagttc
    40081 aattttattg acagtggcat taaagcggtc gtcgtgggca cgcagggcgt tcaatacggt
    40141 ccaaaccacc ttgtaacgtt cgttatcgtc gagcgcttgt tcgggctcaa catcggcggg
    40201 cacaattacc gggataataa tatagccata ttttttgcct tcggccctgc gcataacacg
    40261 cccgaccgat tgtactacat ctacctgcga gttacgtgcc gaaaggaaaa gcacggcatc
    40321 taacgaaggt acatcaacgc cctcgctcaa aacacgcacg tttgtaataa tgcggcattc
    40381 gttattgtgt gtgtcttctt tgagccatgc gagcatctgg tcgcgttgcg gggcagccat
    40441 ggtcccgtcc atgtgccggg catgcacggt aaccattttt tcttttttct cccggggcaa
    40501 cgaatccaga taattttcgg cagcaaggtt ataacttgcg gcaatgtttt tagaatcagg
    40561 aattttacca caaaaagcaa ccgcccgttt catggggctt tcgtcgccat ctgttttgat
    40621 ggcctcgttg cccagaaatt gttttgataa ggcattgacc gtcccgatga gcttggtaag
    40681 gtcgtcggtt tttatttcgg tttcgccatt ggtaatcata ttttgtacag cgggcgggac
    40741 gtctttgtcg tttagtgtca ggataatcac tttgtaatcg gtcagcaagc ccttttcaac
    40801 tgcttcgcca aaacctatgc gatgaatttc tttgccgaaa tactgttcgt tgtccatcga
    40861 ccacaaaggg atatcctgtt ttgcagcctg cgattgagca tcaatactgt acaatcgggg
    40921 cgtagctgtc atatataaac gtttttttgc ttttataaaa ttttcatcat gcacttttac
    40981 aaaagccgaa tcgtccatgc ccggttcggt atagccagtg gtacggtgtg cttcatcgca
    41041 aataatcaaa tcaaattcgg gaaaaccgtt tttgagcaat atcttttgtg cattggctat
    41101 tacttcaata gattgatagg tagagaatac cactgtcatt gcggcattgc cattttgctt
    41161 ataatgctca aattgtttga gaatatagtt tgcatcggtc gatgcaggcc aggccaaatc
    41221 aatggtactg gttaaatcct ggtcggcgct gtttttgttt ttcgttattt cggggtcgga
    41281 gcaaatacaa atggggttga tgggttcttc ggcctgcgag gcccattcgc gcaacgtttg
    41341 gccaataagg gctatggaag gcactaaaaa aagtatggtg cctttgttgt tggtttgctt
    41401 ttcggcaatt tttaacgagg tcatggtttt gcctgtgcca catgccataa tcagccgtcc
    41461 gcgttcgttc tctttaaagt actccactac tttatcgcgt acttcgaaca catggggata
    41521 tatttttttc ttttctaccc ttgctttctg accatgaata ccttgttcga gtgcttccca
    41581 atcaacaggg gcttctatta agtcgtgtaa atttaacctt gttaccggag gtttttgatt
    41641 tttaatggct tccagcgcgt ttgggcccca tttgttggtg gtcgaaatcc aaagcctctg
    41701 ggcaaagctt gttgttttcg aatcttcgcc cctaaattcg cggcccgaag tggcaagaaa
    41761 actgtccacg gcaggtttat caatggtagc cgtttcctgg taacatttgc attgaatagc
    41821 ccagtattcg tcgaaatggg taactgccac caaatctatc cctgtatcgg ccccgcccat
    41881 atcgtctttg ccgggaaact cgtcccataa ccatactttc ctaaattggt gggcatattg
    41941 cgggtcggtg agcaaatatg cctgcattaa acgttcaaaa cggtcgcctt tatctctttc
    42001 ggaaaaggag attttacggt atttgtctaa tatttttaaa aaaatcatgc tataaatgct
    42061 atgtttttat gattaaaaat ggcacgacta aaatatatat ttttactgtg gtttggtaaa
    42121 aatatcagga ttaaagtctt ttagcagctt taagttttta acccaaatta atcattagga
    42181 ggctttttaa aaccgaacta tttgatttag tcgggttaat cttcccgtta taaacgggac
    42241 aagctgattt aaaaattgtt tggtctaatt tttattaaat taatgaatta ctatttctta
    42301 atcgattaag ctttttgatg cttaattggg ggtaaatcag cctgggcaat aaaaaagccc
    42361 tgctgaaatg cttcaacagg gccttgtatt tatttccccc gcctttgcga aggataatta
    42421 gcgaaaagct atttgccggc tttaatagca gcctgtgcag ctgctaaacg ggcaataggg
    42481 acacggaatg gcgagcaact aacataagtc aatccgtttt tgtaacagaa ttcaacagaa
    42541 gcagggtcac caccttgctc gccgcaaata cctactttca ggtcaggacg ggtagcacgg
    42601 cctttctgga ctgacattgc aataagttgt ccaacgccat cctggtctat tgtctggaat
    42661 gggtctgcag ctaacatttt cttgtcgaga taatcgtgca ggaaacctcc aatatcatcg
    42721 cggctgaaac caaaggtcat ctgggtcagg tcgttggtcc caaatgagaa aaactctgcg
    42781 gtctttgcca tacggtctga caataaagca gcccgtggaa tttcaatcat tgtgccatat
    42841 ttgtaattga ttttgcttac gcccatttta gcgcatactt ctgcatatac gcggtcaaca
    42901 atctttttgg taaaatctaa ctcagaaaca tcgcaggtta ctggtaccat gatttctacg
    42961 tgtgggtctt taccttcttt gctcagttcg gcagaagctt cgaatatagc acgtatttgc
    43021 atttcagata cttcaggata ggttactccc aaacgtaccc cacggtgccc catcataggg
    43081 ttgctttcgt gcaacgcttc gccacgtttt tttatagctt caacagatat acctaatgct
    43141 ttggccaatt ctgcttgttt ttcggcacct actggtacaa actcgtgaag tggagggtca
    43201 agcaaacgga aagttaccgg gcaaccatcc atggcaagca ctgtagcttt aatatctttc
    43261 ttaacaaaag caaacaactc gtccaaagca gctttgcgtt cttcaacccc gttgctgaga
    43321 atcatcttgc gaagcaagaa caatggctgg tcgctacctt cgccataaaa catgtgttct
    43381 gtgcggaaaa gcccaatacc ttcagctcca aagtcacgtg ctattttggc atcagaagga
    43441 gtatctgcgt ttgtacgtac acccatggta cggtatttgt ccaccatttt cataaatgtt
    43501 tggaaacgcg gattttctga agcatccatt aattttaatg cccccaggta aacatttcct
    43561 ttggtaccat tcaatgtaac aacatcccct tctttcaata caatatctgt atctgctatt
    43621 ttagctgttt ttgcctctac attgacatgt aaactccctg ccccaacaat acagcattta
    43681 ccccatccac gggcaacaag ggctgcatgc gaggtcatac cgccacgggc agtaaggata
    43741 ccttcggcag ctcgcatacc ttcaacgtct tcagggttgg tttcttcgcg aagaagaatt
    43801 actttttcgc ctttacggtg ccaggcaact gcatcttcag cagtaaatac tattttacca
    43861 acagcagcgc caggtccggc aggtaagccc ttggtcaata cggatacttt cttttcttct
    43921 gccgggtcgc aaattgggtg gagcaactcg tctaactgtg ccggctcaac acgcataact
    43981 gccttctttt cgtctatcaa cccttcggca agcatgtcca tggccatgtt caaagctgct
    44041 gtcccggtac gtttaccagc acggcattgt aacatgtata gcttgccttc ctgtatggta
    44101 aactcgatat caagcatgtc tttatatgct ttctcaagtg tattacgaat ttcaacaagc
    44161 tctttatatg tagaaggcat agcttcttcc atacttttga ggtgcttgtt ttgctcgttt
    44221 tttgtgtcat cgttcaaagg gcttggggta cgtatccctg ccacaacatc ttcaccttgt
    44281 gcattgacca accattctcc ataaaatttg ttttcgcccg tagccgggtt ccgggtaaaa
    44341 gcaacccctg tagctgatgt gtcgcccatg ttgccaaata ccatggcctg aacgtttact
    44401 gctgttcccc atgaatcggg aataccttcg atacgacggt acgaaactgc tttcttcccg
    44461 ttccaactct taaatactgc tttgataccc ccaattacct gttctttagc atcatccgga
    44521 aattcttttc caagtacctc ttttactttt ttcttaaaat cttcagatag ttgcttcagc
    44581 tcatctgcag tgatttcggt atctgatttg taacctcttt tatgtttaaa atcgtccaac
    44641 atgtcgtcaa gctgcttgcg gataccttta ccatctttgg gctctatacc ttcagctttt
    44701 tccattacta catcggcata catcatgatc aaacgacgat atgagtccca tacgaaacgc
    44761 ggattgtttg tttttttgat aaatcccgga atagtagctg tacatagccc tacgttcaat
    44821 actgtgtcca tcatgcctgg cattgatgcc cgggcccctg aacgacatga aaccagaagc
    44881 gcattttctg ggtcgccata cttcatcccc ataacatctt ctactttttt catatttgcc
    44941 caaatttcat catatagttc tttaggcaac tcgcaattgt tggcataata ctcggtacat
    45001 acatcggttg taactgtaaa acccgcaggt acaggcaatc caagtaaaca catttcggcc
    45061 aagtttgccc cttttcctcc cagcaggttt ttcatgtctg ccttaccatc ggcattcttt
    45121 gcgccgaaga aataaacacg ttttgtagtc atagtttaat aatctttaat tatttgatag
    45181 ttagatattt aatagaattt gtataatttc atttattaca aatgtactca aaaatcaatt
    45241 taatttgatt tctcttgcat tttttatcat tttttttaat acaaaaatcc ttaggattat
    45301 tggctgctta aatcactttg tttaaaaaaa ccttaaaata ttgatttaca gcgtatgtct
    45361 tttttaaaac ggctatcatt cttatctttt gcttattttt ttctaaaata gatttttgtg
    45421 ctgtatatct aaaaaatggg agagcccaaa ctcgcatatg ttgatataca actacaagtt
    45481 cttgtggctt tatcgggcct tcaaaattta attttgttca gataaaaaat ataattattt
    45541 ttgcggccac ttacccaaat ggcggaattg gtagacgcgc tagtttcagg gactagtgtc
    45601 cgaaaggatg tgcaggttcg agtcctgttt tgggtactaa aatgttcatt tgctattgat
    45661 gtttttaaat tgttaattgc atatttaaaa tagatgtata tctttgcggc aaatttgaca
    45721 ttcatcaatc aatagttgtc aatgttaaat gcccaggtgg tggaattggt agacacgcca
    45781 ctttgagggg gtggtggggg ttaccctgtg tgagttcgag tctcgtcctg ggcactaaaa
    45841 aattaaagct ttgtaaacaa tagtttgcaa agcttttttg ttttcgctgc ctaagtcttg
    45901 aatatgcaaa aagaaagtgt attgtctgct aataaaacaa aaaagaggcc ggcattgctg
    45961 ccagcctctt ttcagtgatc tcgctgggat tcgaacccag gaccccatcc ttaaaaggga
    46021 tgtgctctac ctgctgagct acgaaatcat atcgtttttg atggcgcaaa gatagtatct
    46081 atttttgttt tgccaaatct gaaatgcgtt tttgtcaaaa aaataaatat ttgatgtgtt
    46141 tatgcttttg aatgagcctt aaatacttaa taaagcaaaa aagaccagca tcaactgcca
    46201 gtcttttcag tgatctcgct gggattcgaa cccaggaccc catccttaaa agggatgtgc
    46261 tctacctgct gagctacgaa atcataccgt ttttggtggc gcaaagatag acagtatttt
    46321 atttctgcca aaacttttgc accttttttt gtaaaaaata ctaagactat attatattgc
    46381 tcaatatctg gtagttgtaa taaatacttt ttgccattat ataagcacta agtgctctgc
    46441 gtgcattatt ttttcgcaat aatgacattt taatgatacc ggtttttttg ttaatacctc
    46501 aaagcgagtg gtaatagctt cgttattggt aatgcattta ggattaaaac aacgtgcaat
    46561 gccttttact tcatcagggg ctaccaatag ttttttttct accacttcat attctttgat
    46621 gatattgagc ttggcttcgg gggcaacaag ggctattttg cttatttctt cctgttgaaa
    46681 gaatacgtca gatattttaa taattgcctt agaaccaagt tgcttgcttt cgaggttggt
    46741 gccaaaggta atctgcgttt ttatcttttc cagtttaagg atagaaatga ccttaaagag
    46801 gttctgtgcc ggaatatggt ctattactgt cccgttgcgg agggcactta cttttaactg
    46861 ggttttatct ttgctgtgca ttttttttaa attaatccta atattgaaca tataatggcc
    46921 tggcgcataa ataccccatt gcgtgcctga tcaaagtaat aagctttatg gttgtcgtcc
    46981 acgtcaacat ctatctcgtt tacacgtgga agagggtgca gtactttgag gttatcctta
    47041 gtattccgaa gcatttcatt gttcaatata taggcattct ttgttttttc gtattcaatc
    47101 gggtccgaaa acctttcttt ttgtaccctg gtcatataca tgatgtccac tttatcaata
    47161 tattcggcca tatcggtgtg ctcgtaatac ttcatcccgc gctgggccaa cagcatttta
    47221 tattcatccg gcattttaag ttcgggcggc gaaatgaaat aaaaagtagt attgaatttg
    47281 ttcatggcca tcagcagcga atgtacggtt cgtccgtatt tcaagtcgcc taccatcatc
    47341 aggttaatat tttcaagttt tccctgggtt ttttttatcg aaaacatatc cagcagggtt
    47401 tgtgaagggt gttggttggc tccgtcacca gcatttataa taggtacatt agatatttca
    47461 gaagcaaacc ttgcagcccc ttctaaaggg tggcgcatga ctatcaaatc gcaataactg
    47521 cttactattt taatggtgtc ttttagcgtt tcacctttgg tcacgctgga tgaagatgca
    47581 tccgaaaatc caattacgcg gccacccaaa cgggtaacgg cactttcaaa actcaatcgg
    47641 gtacgtgtag atggctcgaa aaacaaagtg gccacaactt tattccttaa tttgtctaaa
    47701 gggggctctt tttcaaattg aaaggctaaa tcaataagtt ttaagtgttc ttcctgcgta
    47761 aaatcattga ttgaaactag gcttttgttt ttcatttgtt gctgattctt agagtttatc
    47821 tggtatatga agccttaatg cctgcttttc ttttccccga taaaaatagt gaatattttc
    47881 tattttgtat ttttatacag gacttctttt tcaacaaaat acattaatcc ttaaatttga
    47941 ataataatat tagatgtatt ttgtaatata ttttatatca atattttatg taactctatt
    48001 tgctttattt ctccattgta ttctgctttt aatacaaaac tgaaacggtt caggtcaagg
    48061 cacatgtcaa agtctgaaga aggggagtgg gcctggtttt caggtaaaaa caatcgttga
    48121 cctgaagtct gccttattgt tttcttcatt tgttcaaaat caggcttaga accttctaag
    48181 tattgtttga tatattccgc gcagaatgta tcttcttcgg ttggcctttg ggctgaatag
    48241 cccatacaaa ctaatgaaac cacatcaggc ttttgctttt ggatatattt tgctatagct
    48301 tcagcattaa caaagcttcc tgtaataatc tgatcggcat ttacagcatt gataattcct
    48361 tgagtcccgg cactggtagt atgtataaca gttttataat caaaatcata ctggctgata
    48421 tgcgtggggc tgtttccaaa gtcaaaaccc tcgggaatac gctcatttcg ttcgccaatc
    48481 aggataaaat ccgggtgctg ttctttgata gcgtaggctt cttccaactt tcccacagga
    48541 aatatccttg ttcctttatg gtccatcaca taacaggcca atgaaaaagc cctgaaaacg
    48601 tcaataacaa cggtcagccc tttggctttt ttagctcctt cgatgagttg caatatttgt
    48661 atttccatta tttttatttg tttcgcaaag gtaaaaaata tcaaggcttt tataacaatg
    48721 tgctttgata tctgtttatg tttctataaa tttacctaaa aaccaaagat atgtaccgaa
    48781 aacttattct tgtcattaat cctggttcaa catccactaa ggtggctgtt tatcataata
    48841 attccccgct ttttctgaag aatatcaagc ataatgctga acaactgaaa gattttcctt
    48901 ctatgagtgc tcaatacgag tttcgcaaaa acttcattac ccgcgaactc gggcatgccg
    48961 agattgactt gtgtgatatt ggcattatta ttggccgtgg tggacttatt aagcctatag
    49021 aatccgggat ttatgaagtg aacgaatcta tgtacaacga tttgctcaac agccctttcg
    49081 gacagcatgc cagtaacctt ggaggattga ttgcttatga tatggctaaa gcattaaaag
    49141 gggtaagggc cattattgcc gacccggtgg tggtggatga attgagcgat gtagcccgtg
    49201 ttgctgggca tccggagttt aagcgttatc ctatctttca tgcactaaac caaaaggcca
    49261 ttgcccgcaa ttacgctaac tcgcatgacc agaaatatga agaactgcat ttgattgtag
    49321 ctcacttagg tggtggtatt tctgtcgggg cacacgaaaa gggcagggta gtggacgtta
    49381 acaatgcgtt ggatggtgaa gggccaatgt cggttgaacg tagcggaact atacctgccg
    49441 gacaacttgt gaaaatatgc ttctctggca agtacgacca gcaaactatc gaaaaaatga
    49501 ttgttggaca aggaggcctc gtggcatacc ttggtaccaa tgatggttac gagattgagc
    49561 ttatggctcg cgaaggtaat gaaaaagcta aaaccttgca aaatgccttg agttaccagg
    49621 tgggaaaagc tattggcgaa atggccgctg tgctcaaagg caatgtagat gccatattgc
    49681 ttacaggggg aatggccaac aatagcatga ttactgacta tataaaatca atggtgaaat
    49741 ttatagcacc ggtcatcatt tatcccggtg aggatgaaat gaaagctatg gccgataatg
    49801 cgctcagggt tttgcgtggc gagataaccc ctaaaaagta tatatagaaa aatgcctttt
    49861 attttaactc atcttgcgaa agtcctgttt atagaataca agttgtcatt tattgtttaa
    49921 aatcattgaa tagcaataaa tacaaagtct catgcaactt ttttatcgaa aaaagttgct
    49981 caaaaactct tatcgcctta actctttcac cctcgaagcc tcttaatgtt gcaat
//
LOCUS       DAOSPF010000021        48445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_789_length_48445_cov_7.385927, whole genome shotgun sequence.
ACCESSION   DAOSPF010000021 DAOSPF010000000
VERSION     DAOSPF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_789_length_48445_cov_7.385927"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK252_05980"
     CDS             <1..230
                     /locus_tag="PK252_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14291.1"
                     /translation="QKKDKRQEQMEKKQEKEKKEQEQYAKAQQHQLDIQSKKTRERML
                     KNKKMTDDYYKKKARHRCWRKFVTFVQQNFP"
     gene            234..716
                     /locus_tag="PK252_05985"
     CDS             234..716
                     /locus_tag="PK252_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14292.1"
                     /translation="MDADCNFIPFLVVNKAGIDGGTLFYIIIAVIVLISGFIQKKNAK
                     NNQEPSKPEHAPDPWKEIFDFPEMDEKPEPEYKPEPVKVEVQKKPEVKKAPEPINPFL
                     SQETLEYLKTREGTVTTFDDVIKQSEIGSAFESEHYKPIEIDLKKAVIYSEIINRKYH
                     "
     gene            875..1348
                     /gene="greA"
                     /locus_tag="PK252_05990"
     CDS             875..1348
                     /gene="greA"
                     /locus_tag="PK252_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT14293.1"
                     /translation="MSKVSYITQEGLQKLKAELEHLINVERPAISRQIAEARDKGDLS
                     ENAEYDAAKDAQGMLEMRISKLEQLLANSRVIDESKIDTSKVQILNKVKIKNTKTNAV
                     LEYQIVSETEANLKEGKIAANSPIAKALMGKKVGDVAEAIIPAGKVMFQILEISR"
     gene            1368..2405
                     /locus_tag="PK252_05995"
     CDS             1368..2405
                     /locus_tag="PK252_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOT14294.1"
                     /translation="MENFEFVGYRFPAEWERHEATWLTWPYREESFPENLHVVYEQYV
                     CFIGQIAKSEKVRINIPDEGQKAVLYSWLEKLSLGDENIQCFIHPSNDVWCRDHGPAF
                     IVNPKAVYPKAIVDWEFNAWGGKYPSNFDNQIPQFIASTFSLPYVSPGIVMEGGSVDF
                     NGEGDLLTTTACLLNKNRNPHLSQKQIEEYLRKYYGVHNIIWLGDGIAGDDTDGHVDD
                     ITRFVDANTIITVIEHDKSDENYEPLKENARKLAKVRLANGKQPDIVELPMPKPLYYK
                     ADRLPASYANFYFANSALIVPVFNCDADEKVLEILSNLIKDRPVVGIDSSNIVRGLGS
                     FHCLSQQEPLV"
     gene            2427..3830
                     /gene="rlmD"
                     /locus_tag="PK252_06000"
     CDS             2427..3830
                     /gene="rlmD"
                     /locus_tag="PK252_06000"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT14295.1"
                     /translation="MGRKKSLPVIEDVEIIELAAEGNAIAKIDGKVLFVPKAIPGDVV
                     DVQLTRKRTSFMEGYVVNYKRYSQHRIEPFCSHFGTCGGCKWQHLPYTEQLRWKQKQV
                     TDNLQRIGGGQIEKVFNIMPSENTRFYRNKLEYTFSNNRWLPAEMIKQPDFVREPGAG
                     FHIQGMFDKVLDLDYCYHQPDPSNEFRLALKQFLLDNNLSFFDIREKKGFLRNLVVRN
                     TVAGQWMLIVIFGEDNPQQITKTLTYLKDRFSALDSLMFTVNTKLNDTFHDLDIKLYY
                     GKPYIVEKMEDLQFKIGPKSFFQTNTQQAVNLYSKVREWASLHSDDIVYDLYTGTGTI
                     ANFLAPHAKRVVGIEYIADAIEDAKENSKLNNITNTLFFAGDIKDLLNDSFFNEHGRP
                     RVVVTDPPRAGMHPDVINALLGALPETIVYVSCNPATQARDIKLMADKYKVVSIQPVD
                     MFPHTHHVENIALLSLR"
     gene            3827..4600
                     /locus_tag="PK252_06005"
     CDS             3827..4600
                     /locus_tag="PK252_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14296.1"
                     /translation="MTEIDISAKRLLEKLAKKVGKTILNHGLVQPGDRILVAVSGGKD
                     SYALLHLLTETLRHAPFKPYIVAIHVVNKDIGYQIDLDFMKSFCLYLGIDLYIEESTV
                     DLTVKSKKSPCFICSWERRKVIFQKAREFQCNKIAFGHHLDDAIQTLLMNMIYHGSIS
                     SLPYFVKMFQGERTLVRPLLDLDEQILSSLAKMMNYPVEVKQCKYSDTTKRDAVKKII
                     EELNSKYPLTGKNLFKSMQNCFHEYLPELNQTLNKSPET"
     gene            4870..5823
                     /locus_tag="PK252_06010"
     CDS             4870..5823
                     /locus_tag="PK252_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT14297.1"
                     /translation="MKRMKSKIWIAGLVALLTVSCNYDPRAGINEETPTRGNIKIGVD
                     ESFYLLSEAHLAVFQSIYENAHIKPLYKPELDVLNDFLNDSIRLMVTSRKLTTEELEY
                     FKSRNIFPQTVTIAYDGIAFIVNKTNKDSLIRYNQIRDIFLGKIDNWNKINPKNTNGA
                     IKVVFDNIKSSNVRYLNEKFKVGSKFPKYCYSVNTNEEVINYVEKNKNAIGVLSVNWI
                     SDKDDSITRGFLKRIKVVGVTPENDPEGPEYTKPYAGFIAEKSYPFIREVYMINRESF
                     SGLGSGYLQWVTADQGQRIVLKMGMVPATMPIRLIKTKSNF"
     gene            5911..7779
                     /locus_tag="PK252_06015"
     CDS             5911..7779
                     /locus_tag="PK252_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14298.1"
                     /translation="MKIFSFRISMYWCIAALFLSLGSALLQAQDINQALKYLQSERYE
                     DANAIFQKLIDSNPTEANACFYYGESILLNFQNDPYSNTLEEVVKESATYFNMGIKRD
                     STNMLNYIGLGMLKLLQHNDTIGADIYFTKVENVIPKKEKKFTDNDFNLLIKLAMAQL
                     YSPKPRYNKAIAYLERAKAASVVIAQKTKKEVPSVYIALGDIYNKMNNASLAVANYNK
                     AVYIDDKLYEPIVKIGRLYMRSRNLQEARNYFDQAKEIDSTYAPLYRAYGEMYAMGGL
                     ANLSKQNFKKFLDLSGNNIPAKIQYVNSLFKAKDYKTCVENIEEIFAVDKSRNYLNRL
                     AAYSCYEMRPADYEKAKMYIEEFFKNSSPEKIIPKDYTYYGRTLLKMKDTTLIMKAFE
                     QLQKAYSFDTTDYALLTDIAVDAYYAKQFKLAVETFNKKIASGQAEISDYFTLGKTYY
                     QMAQGEKDTAIQRAAYNDAIKTFTTITKLEPSNVEAYLWIANTSFGLDPESKLGIAKP
                     AYEMVIEKGSADTIKYAPQLFAAYSYMGSYYLFAAKPDYAMAESIFWKMVWLDPKKKE
                     WQVKGYYSLGATYTSRKNYAVAQTYYKKVLEIDPKNEAALKDLESINKVLKAKQNQ"
     gene            7876..8676
                     /locus_tag="PK252_06020"
     CDS             7876..8676
                     /locus_tag="PK252_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT14299.1"
                     /translation="MKALKEIFSSVFNTLVILVCLAIGITIYMKVLGNPINFMEGNPK
                     GHPLPGNYLGMMYKGGPIVPVLMTVVLVLLTFTFERFISLSRASGRGSVGKFVRNIQT
                     LLNENKIEEASAACDKQRGSLANVVKAGLVRYRDLQNDTTLPKDQKVLALQKDLEEAT
                     ALELPMLSKNLVILSTIASISVLIGLIGTVLGMIRAFSALATAGAPDALALATGISEA
                     LINTAFGISGSTIAILVYNYFSSKIDAMTYKIDESNFSVVQTFAATLK"
     gene            8727..9350
                     /locus_tag="PK252_06025"
     CDS             8727..9350
                     /locus_tag="PK252_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT14300.1"
                     /translation="MSKIKMTVRTPRIDMTPMVDLFMLLLTFFMLTTTFRPSEAVQVD
                     TPNSISEKTAPDKNVITIFISKDNKVFFNVDEGTDTSQHIRRQVLKSISEHFRIPFTE
                     EQVAKFEKLASFGMPVKNIPAWIDAEQKQRDEMQVGMPYDSVDNQLALWVLFARNANP
                     NAEAAIKGDAEADYKVVKRVLDILQDYKINKFNLTTNLEKVEVKLEN"
     gene            9365..10024
                     /locus_tag="PK252_06030"
     CDS             9365..10024
                     /locus_tag="PK252_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT14301.1"
                     /translation="MAEIIQDEGGHKKGKRRAKKHSTHIDMTPMVDLACLLLTFFMLT
                     TAFSKPKAMEIVLPDKVKDEKPDPKNESKIPKYRAVTIMIDENDKVYYYTGLMEKEPY
                     PEIVETDFSKDGLRKFLLGRNKKLYKQIDSLNTAVLKSKKAISPDTVVKMKRKLRKED
                     KAGPIVLIKATEKAKYKNMVDVIDEMAICEIPIYAIVDMTPQEKKMLEAKKKGTSTTK
                     Q"
     gene            10036..10827
                     /locus_tag="PK252_06035"
     CDS             10036..10827
                     /locus_tag="PK252_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT14302.1"
                     /translation="MSENNINISNQAEFLELDEIVFSKRNKEYGAYALRKRYKKVLLI
                     AFSGAFLIIGTVVAVPLIEAYRAKSKALKRIDKNVSLEMEKLNQEDAPPPPPPPPPPA
                     ALEQQVKFRAPVVVDTVKEEVALATVEEQAEIVNETPPEEIVVEEKKKDEVIEAEEPA
                     FVIVEENASFQGGDINSFRIWVQQNIVFPTAAAEAGISGKVIVQFAVNSRGQIVDAKV
                     LRGVHPELDKEALRCIMGSPKWTPGKQGGKAVKQQFVIPIVFTLQ"
     gene            complement(11249..11839)
                     /locus_tag="PK252_06040"
     CDS             complement(11249..11839)
                     /locus_tag="PK252_06040"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOT14303.1"
                     /translation="MAENVTIVKDTTANPAPLGLLGFGMTTLLLNIHNAGFYDLNSMI
                     MGMGLFVGGLTQVIAGIQEWKKNNTFGATAFTAYGFFWIALVAIWLIPRTSFGADLKS
                     DETSMGWFLLIWGIFTLGMFIGTLKLNRALQLVFGTLVVLFLLLAIGDFTGIKEIKTV
                     AGYEGIICGLLAIYTCLAQIINEVYGKTILPIDSKK"
     gene            complement(12111..13103)
                     /locus_tag="PK252_06045"
     CDS             complement(12111..13103)
                     /locus_tag="PK252_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT14304.1"
                     /translation="MKVLFIGGTGNISSAVSKMAVSKGINLYLLNRGNRDSIEGVKQM
                     HTDIHDVIKTKQLLSTEKWDVVVNWVAFTVDDITRDFELFANSTKQYIFISSASAYQK
                     PPVNHIITESTPLVNPFWEYSRNKIACEEQLMHLYRTKGFPITIVRPSHTYNTVIPIT
                     LGGWTEYTTVYRMKKGLPVVVQGDGTSLWTLTHAKDFARGFIGLLGNIHAIGEAFHIT
                     SDEILSWNQIYETLAEAAGVKANIVHIPTKQICQYADKTGYPSEFGSLWGDKSHCAIF
                     DNTKIKQFVPEYKVVIPFSEGIKETIKWFEAKPERMIINEATNNLIDGLIKIWG"
     gene            13364..13621
                     /locus_tag="PK252_06050"
     CDS             13364..13621
                     /locus_tag="PK252_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOT14305.1"
                     /translation="MTITFNELRKIKDSLPSGSMKKIADKLNIDEDTVRNYFGGTHYK
                     NGEGKTVGVHIEQGPDGGIVTFDDETILSVAREMLAESAHH"
     gene            complement(13651..14838)
                     /locus_tag="PK252_06055"
     CDS             complement(13651..14838)
                     /locus_tag="PK252_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14306.1"
                     /translation="MTRLLAFILLFSSLYLPAQTSLQKAVAYSQQNTEDSANFYFSKA
                     IEETPNNYLVYLRQGMFLYSLKKDAKLPLLKANTLRKNSASFYLALVFASERDTLNTI
                     KYLKQNLSSEYQVKPSDIKMEKAFSFLEKTEQWKTLWEQNWYNKNDELIGELLFLYHS
                     GNYMEVINTVSNFAINNQKISHEMYYLRACSYLALGNYNAALSDINEAIRINKHRSKY
                     FELRADVKIKLKQYVQASKDYKEAKRLDLLNFEAICKNAYCFLLQQQYEQSLEEIAIY
                     IYLLPQNEKGLYIGAQAAFHSNKYIKSLEYLNTLFKAGYNNTDYYILRAKCYAQAQLT
                     NNALQDLNTVLNKQPHNISALLERGNIYYSIKQFSQACEDWKKAKSLGSLQADKLLWM
                     NCK"
     gene            complement(14841..15836)
                     /locus_tag="PK252_06060"
     CDS             complement(14841..15836)
                     /locus_tag="PK252_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT14307.1"
                     /translation="MNTIGTLFKLSIFGESHGNSVGIVIDGVPAGIPITREELEADLS
                     RRKSGAKGTTPRQEADIPRIMSGVFENHTTGAPILILFENTNTRSKDYSFLLETPRPG
                     HADFVAHKKFGGYEDYRGGGHFSGRITLGIVAAGVIAKKIIAPIIVKSTLIEAGGTTD
                     IEKAVEKAIAEQDSIGGIVETIATNMPIGLGEPFFGNIKAVLAHYIYSIPAIKGLEFG
                     SGFAAARMKGTEHNDKFITVSGKTATNYAGGINGGISNGNDLVFKVAVKPTSSIHATQ
                     HTMNMKTGQMTDLSVEGRHDTCIALRIPVVVEAMTCLALADLVLQEQKFPRVVKS"
     gene            complement(15864..16502)
                     /locus_tag="PK252_06065"
     CDS             complement(15864..16502)
                     /locus_tag="PK252_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOT14308.1"
                     /translation="MICVSLNITDVDQCIEALKSLEMAEIRIDLAGYNEQQVERVFSS
                     HKNLIATCRPDNITDEKRLKLLIRAIESGAAFVDVEYESDLSFKHAVKQAIQNKNCKL
                     IISFHDFKKTPTREQLEIIVQQCFDMGADIAKLACMVNTDADNAALLSLYDSEKPVVS
                     LGMGEKGKISRIAAPLLGSPFTFAAFDEQSATAPGQIQYKRLAELYSFIKNS"
     gene            complement(16483..17520)
                     /gene="aroB"
                     /locus_tag="PK252_06070"
     CDS             complement(16483..17520)
                     /gene="aroB"
                     /locus_tag="PK252_06070"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT14309.1"
                     /translation="MEKVVLNTAGAVSNILIGESVYNLAKYIPANQKTIIITDDNIAK
                     LYAKAIEGFDVIVIPQGENNKTLATIDLIMGKLVEFEADRSTFIVGIGGGIVSDITGF
                     AASIYMRGLNFGFVTTSLLSMVDASVGGKNGVNYGGFKNMVGVFNQPQFVICDIEMLK
                     TLDREEFIGGMAEVIKHGAIKDKALFEFLENNYNAILKYDKDAIHRCIKDSVEIKANV
                     VSQDEREKGERRKLNFGHTFGHAIEKLTMMPHGKAVAIGMNMAAAASVLLGHLSQNEA
                     LRLKKLTENFLLPTYLDADSNALFNAMKKDKKRDGASLHLVLLDSIGHAIIENVEYKK
                     LEEIIHDMRLA"
     gene            complement(17522..18622)
                     /locus_tag="PK252_06075"
     CDS             complement(17522..18622)
                     /locus_tag="PK252_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOT14310.1"
                     /translation="MESQLKLQPFTFENVPSTRPMIIAGPCSAESEQQMVETATQLAA
                     QGIKIFRAGIWKPRTRPGSFEGYGSAALPWMKSVKQATGMLTATEVANVRHVYEALKA
                     GIDILWIGARTSVNPFAVQEIADALRGVDIPVLIKNPVNPDLELWIGAIERFYHAGVT
                     RLAAGHRGFSSHEKLVYRNDPNWQIPIELRRRIPNIPMFVDPSHICGNRQYLGEICQK
                     SMDLNYDGIIIESHINPDVAMSDAKQQLTPADLSVLLSKLVLRKEYADNNSLVTLEEL
                     RAQIDKLDDQLVDLLEKRMKVAEQIGVCKKENNIAILQTSRWDKIITKRLEEGAKKGL
                     SAQFIETIFRSIHEESIDHQTKVMNEPKNGNK"
     gene            complement(18690..19553)
                     /locus_tag="PK252_06080"
     CDS             complement(18690..19553)
                     /locus_tag="PK252_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOT14311.1"
                     /translation="MTPKTSKQTSRVAIQGGYGAFHEIAAKHYFENENIEIVTANTFK
                     DLFKSLKNNHADLGIMAIENSLAGSILPNYNLLQDSNMKIIGEIYLRIKQNLVALPGV
                     KIEDITEVYSHPIAILQCQMFFDNYPNIKVLDSIDTALSAKDIADKKLKNTGAICSKL
                     AADKYGLNILAEGIETNKMNYTRFLILSEKIGYHKQDKHIDKASITFVLEHQTGSLSK
                     VLSILSYYGINLTKIQSMPIIGKDWEYQFYVDVTFNDYVMYRQALDAIRPFTSRLDIM
                     GEYAKGKTVME"
     gene            complement(19802..22939)
                     /locus_tag="PK252_06085"
     CDS             complement(19802..22939)
                     /locus_tag="PK252_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT14312.1"
                     /translation="MRTRSFILSLCFIALLIAQGFSQSINIKRFNIEQGLSHPSVYSI
                     IQDKTGFIWLCTGVGLNRFDGTTFTNTFMGDTLPNTVASANFQSSKDSLWFGFDDGSL
                     YLYSKRKFVKKYSNPQSPSTITCLTKDNDGNILACTQNNGIILITPEKTETLPGTNDK
                     LIYSIINIGNNHFLVGTSSGLWEAKLDAAKSEFIFRNNIKCIAQTKIQCIASYGQDKY
                     LIGTEDEGISLLQYNSKKTNAQKLSNDQKLKLANIQSIQIDDNNNIWLATFGNGIYKL
                     ANKNNTFEITENYNESNGLGDNYIKSIYFDREKDLWIGTYSNGLSAIINESFVFYNFS
                     KEQINNNILSIASTKDGIFFGSRQEISFMSAKNKQIFTLTTKNGLPADNITAILPIEN
                     NQLYFGTEKNGVYLLNFQTKQISKVFRSNNSVENKINYLTCNKNTLWIATNGGIIKID
                     QNTGRTEKYSMDNGLPHNKINSLFISPDGNIWVASKSNGLVSLDNKKHFKMEGIPEIE
                     FTGIAMDKKGNLWASTYGDGVFCFRKDSVIHLSENEGLKSDYGYFIISDPDGNLWTGH
                     RMGLSRINSNTFKVLTYGIELGIRGDCNLNAANASDEGVLRFGTTDGIVQYNYSSNKQ
                     KLLPPNVDLVSVKIGDKEIDFSKPIVLPYGKYRIRFDFVGLNFTNPAGVKYQYKLEGW
                     ENEWSEISSNTFALYPRVDDGNFTFLVKAYNAEGIANEIPFSVTVKIKAPFWKQWWFI
                     LLSIISLVAAVYAIIKYREQKQIAFQKHLQKLLDERTREVVEQKEEIEIKNRDITDSI
                     TYAQRIQTSILPSIKKLQDSFSGCFIFYQPRDIVSGDFYWFDKVTDTKFSIVCGDSTG
                     HGVPGALMSMIGTTLIKDICTRPDVVKPSDILLKLDEEMANTLNQNFDAEKSSDGMDI
                     IACEIDVESYVVRIASAMRPVILYQNGEQVYVQGSKNSIGGTEFGDQGKDFEDQTFQL
                     QKGDIIYMFSDGYPDQFGGPMGKKFKMIRLRNMLRDIHELPMEEQYLHVKNTFNLWKD
                     QLEQVDDVLFMGIKI"
     gene            complement(23022..24008)
                     /locus_tag="PK252_06090"
     CDS             complement(23022..24008)
                     /locus_tag="PK252_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14313.1"
                     /translation="MNTLIKKHLFILLIASYLPIACFSQAAPADDENIASLITFGKNA
                     ETSWGDNDHVQIFFFLIPEKFKKPVYIRVFDPEASGDDDELNGVFDTETTFSVYGGKK
                     CWSEEAAQSSNPTSGNYKSGNLKATKTFGNTSKYDKTWYTFGPFNPTEGEYVQKFGGN
                     VLKVIAEGISGDDGNKYKFYLSTSPTDNVPIEGANAFAYDYCFRMWDDPKQVSHIYPY
                     VDDKTISVKISNFDWDDDGFIRIVSVARKGQMEAVSGQDNWVTTEFKIIQEEKNTSLD
                     IQMIKRKNAPVKNNNVVINVRNQYGELLPFYTIPIGGVPKYKYSIGVKQKTE"
     gene            complement(24046..25479)
                     /locus_tag="PK252_06095"
     CDS             complement(24046..25479)
                     /locus_tag="PK252_06095"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT14314.1"
                     /translation="MKKIIIYGVFLFLADIIPLNLGFSQRQYTVEKLSFCSNKQNEFA
                     PAFAKDGIIYCTDRKHSFWFSYSDTAENSSQPFDLYQVKLTKDFKDYKSIERLNLNTI
                     FNEGPCCLFNSDKSIVFTRNLDATKKFGNYNKKGNTLGIFFAELKKDEWVNITAFEYN
                     STEYNVMHPTITADGTTMYFASNMPGGIGGYDIYVTHLNNGSWSKPENCGPNVNTDKN
                     EAFPFIHQSGRLFFASKGWNTRGGYDIFFTLKMNGQWIKPENMKEPFSSTADDFGLIL
                     DPYLQTGFFASSRNKNDDIFRVQSAISEFENCTQQKKNNFCYVFFENGTSEEDVKGTM
                     KYEWDLGDGTKIRGLEAEHCFAKPGKYRIQLNVIDSVTNEIMMNQAEYDFNVEEIEQP
                     YITAPDMATIGETITFDAKKSNLKNFKIAKYYWDFGDGTKSFGESISHVFYEEGLYDV
                     KLMLESTQTRTGIKKYCVFKSISIKQH"
     gene            complement(25500..26423)
                     /locus_tag="PK252_06100"
     CDS             complement(25500..26423)
                     /locus_tag="PK252_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOT14315.1"
                     /translation="MKKKLAILSILSIVAINANAQYYPLFSQYLSNGVLLNPAYTGSR
                     DVLSVNAMHRSQMIGFKGAPSYQSFTAHMPLKNENIGIGLMALNEKAGPIQNTHIYAT
                     YAYRVAAGPGKLAFGLKAGLNYGNYGWSNLYTNESGDPAFENNNTTLLLPNLGAGIYY
                     YSNTFFTGLSIPYFLSYKEKNSHDGFEIFHDINNYNFLLTAGFLLNISPNLRIKPSTL
                     LKYNMTAKEQFDINLNIITLNDMLSFGALYRTGEAMAGIVEVQLNHQLRIGCTYEYST
                     PSTNTYNYYSFEFSVRYEFLYKIKAANPRLF"
     gene            complement(26423..30553)
                     /locus_tag="PK252_06105"
     CDS             complement(26423..30553)
                     /locus_tag="PK252_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT14316.1"
                     /translation="MHFTHIHKYNFKKTLIALLLVLLANPIILRTEVINQYYKIDSIH
                     FGAANQLDTIFVTNPSLIKDSDIIILHQAKGVNWSYDSISPVGNNGFTGRFEFHVVHK
                     KISNKILVRSISKDTIFNVSYSDTCNLQLVKVGTLNDITKNKTINNTVTCAPWDGSKG
                     GIVAIVADTLILNANIDVSGKGFRGGNPTGDSKATCWENDSTNVSKGYLPKSKMAFGG
                     FKGETIVSIDTNFTRGRTKLYTAGGGGNGYHAGGGGGACFYMGGNGGYEAPLCDTTPK
                     TLGGEGGLGLGLGLLNYPDKNNPNPAIAVFGGGGGCSTQQFPYVASKGGNGGGIIIIM
                     ANAIKANGYSLKANGESVTGIATAGAGGGGGGGMILIDISTYLDKLNIEIKGGNGGSV
                     NTVPPMPGPGGGGAGGFLRFNNSILSDSIILRKDEGNFGNPTLPFKNASSGTPGEMMG
                     KLIMPVKDFLFNLMPADQEICEGVTPKLIKASTPKGANNKFRYVWRKSTNKILWDSIA
                     NQMTYQPGPLTVTTYYQRIVHPLDENNKVMSAYSDTSTILTIKVWPKIKNNFITLDTD
                     SNVVCKNKQMPLLCRNIKDKEDTIKGGLGPGTYTYLWQDSISAWDSIAPGISNKFTYQ
                     PVLNDSAKFRRVVISGVCRSYSNTIKIDVLPTIKNNIISDTQWVALGANIDTIRGLAV
                     NGGDNSYKYIWKKSFDKINWTTFDSTNNVHDIIWPKPTQTDTFYLYRLVISGKSNTCQ
                     DISNTITIHILDSIRNNRIKGNDTICSLTKPSDFIGSLPKGGDGVYYYKWEQSNNASF
                     SSPQSFDVISDTIFNPGIIKHNTYFRRIALSGPGYCCKDTSNYIYIHTKPAITNNKIL
                     HSLDTTICYNQSLGLIMGTTPLFGDGPYKYFWEIKENGPFSVNNEKDTLKDFTPPSFK
                     DTVQIRRKVISGICQSYSDTLIVKVLSLISNNISGTDTTICKGTDLEKFNALSPKGGD
                     GTYKYLWQCHEENKPWKNCPGNYSVMQYNPPAIDSAIYFRRIVFSGLNDCCKDTSSIG
                     LIKIFELPTSTLTQFDDSLCLGNKIDLSIRLTGKAPIKIKYTNGFETKEKEFNTTGLN
                     TFSETPLIAGVYNYLITSVQDNNKCLAIDMSGTATKHVFEVPVVFAGIDTAVCGDSIT
                     LIATKTIGKGKWSLEVPATFYPADTANKVSIKTSAFGPQNVTWEANNGGCIDKKNIQV
                     TFFQPPLDPSIGENQSGPYLFKTHLKAYLPQLGVGKWISPDNSIIIENNSDPETYVEN
                     LKFGKNQFIWVVTNGVCQATSDTLIVEATDLKIPEGFSPNGDKINDLFEIKGLENVKD
                     ANLTIINRWGKQIYNQPDYKNNWNGTQDGKELPSDTYYYILNVIGRTYKGYIVIRK"
     gene            complement(30611..34339)
                     /locus_tag="PK252_06110"
     CDS             complement(30611..34339)
                     /locus_tag="PK252_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14317.1"
                     /translation="MHETNDNKRMSRRFLTYAILLLSFSLSLDILLKSDFYKNIQLQH
                     FQERFLQVESELDLQLDDISNQLGKTTNDILLSNKIKELGNWAQSRQSYFFIFKHDSL
                     VFWSSNRFVETDILQYLISNRTALQLSNGWCYAAERQNFDYKIIGLVLLKNNYPIENK
                     YLKNTFNNIFNTDENVKMTTSFVNEKYVVFNKFHQKVLALNFDETKRYNLFYELFITV
                     CLLSSLILFFRFMKSILENQNNSIRKILYIFIHALASLFLFILLRKLQIPSFFDNTKI
                     FCNSIISLLGFSVSSGDILVISMFVYFSVSLWHKNIFSINSTLFNNKWLIKSIQYLLL
                     VTTFSLYFLACHVLENIFSDLNINIETNQILEHPELFITSILIASTVFCILTLVIDII
                     GRNLFEKKEPLAGKEFFISAVFTFLIFNIIGRQLSVLHLVIVLIISACIVYIRLIKNK
                     NITIILTLWLVIAFSTYTIIFALYGRQKSQINESKQIAFSLSSEHDKVAEYLLIDMGK
                     KLSLDSTLRLRITNENVDVSWIIRYTKKKYFSGYWDKYDFQLTICQANDSVISQTSSQ
                     KELCSLYFNELLKNKAEPVPQSNFFYLKNSNGRITYLSHIKYYNKNQNVSLYIQLDSR
                     LKSEAPNYPQILLTNENLQSPVTQKYSYAKYYKNQLSYQSGNFLYNTTLVKYPTFSGK
                     YYQTSFSGYDHLFFKPDKDNIIILSRPSIGFYNLMVLFSYIFIFSLLVIFILLFSVDS
                     SSVLPYMKKGFITKIRLSVAGIILLSFITIACITFFFIIKQNRENTYKTIREKMQTIY
                     LEMESRLGNVETIRFNWKSYEFSNLENYLKHLSDIFYANINIYDTKGKLISTSRPELI
                     EKGLVGTYMNMQALTQLKQLNKNEFTQAEWIGKQKYNTIYTPFINKQGKLTAYINLQF
                     YDLIYNQVDDTSSLIQGISNFFVIMVLITMGLAFAFSENLIRPLRVIQENMARIKIGS
                     KNEKIEYNGHDEIAELIEAYNHKVEELEVSAQLLAKSERETAWREMAKQVAHEIKNPL
                     TPMKLSIQQLQRAWDDKHPNLDMYFSKVTATLVEQIENLSNIASEFSSFAQMPKTKNE
                     IINLESTIKQAINLFEHNKVNIILSLQDEKTFMVYADKEQLCRVFINLITNSIQAMHN
                     TQDATIEIALKASGNMAVIDFSDNGPGISDENLGKLFRPNFTTKTSGSGLGLAICKKI
                     IDNANGSIEYLTSNQGAHFRIVIPLYNKDFTTNSLSFQ"
     gene            complement(34350..36311)
                     /locus_tag="PK252_06115"
     CDS             complement(34350..36311)
                     /locus_tag="PK252_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT14318.1"
                     /translation="MMKYFALPIFAILITSSAFSQGNKLKKANDSFAAAEWLKAIEQY
                     RDAYEVVEDKNKKTEIIFQIAECFRHMCDPQHAEMWYKKTIDREFQNPVIYLYYANAL
                     RAQGKLKEAEPMYRKYKELVPDDIRGENGIQSCRIAQQWIDSPNGYLVENFKPANSRA
                     CDNSPAFGRDDFKVVFFASTNESAKGQVREGTGEKAFDIFYTKLDKKDKWSAPVSVGD
                     EINTEFEEGSPSINKSFTKMYYTSCKPVKGKKASAHIMVSTLSGETFGTPEMLDIAGD
                     TVDVIHPAISNDEKTLYFVSDMPGGFGGYDIWKVTTNEDGKWGNPENLGPEINTPGNE
                     LYPYSHADGTLYFSSDGHLGLGGLDIFKAKNENGKWTVENMRSPINSYADDYGIVFQP
                     NEERGFFSSGRDKKDIDLYSFVLPPLRFNIAGKIIDDRSEKPLADALIKCISSDGITT
                     DTKSGPDGIFKFALKPNTDYVFIAFKEGFLNNKARQSTKGEVKSKDFTAQINLTPIDK
                     PIEVPDITYDFARAELRPESKTALDKLVETLNDNPNITIEMMANTDYIGNDKANTELS
                     QRRAQSVVDYLISKGIAMDRLSAKGNGESVPKVVDEKLNKQFPFLPVGTILNETFIKT
                     LSPDNQEFANQINRRTEFKVLRTDYVPKQ"
     gene            complement(36459..37481)
                     /locus_tag="PK252_06120"
     CDS             complement(36459..37481)
                     /locus_tag="PK252_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOT14319.1"
                     /translation="MPKKNRILWLVLPIVSIIIFGILGYIIIEHYNFRDALFMTIITI
                     TTVGFREVHPLSDIGMYFTSFLIIISFGIFAYTITALTQYIVDSVFRNYFKIKKMQKK
                     IDKLSNHVIICGFGRNGKQALIELTMHKVPVVVIENEAAQIQQIQECQYEVLFIEGDA
                     TQDETLELAGIRRAKALIAAINSDAQNVFLTLTARELNPKLTIIAKASEEGSDIKLRR
                     AGADNVIMPDKIGGRRMARIVAQPDVVEFVDNIIQQGDTNVLIDEIPLQNISDYLNGR
                     ALRDFESYNDTGAKIIGIKQQNKIFLVNPLPETILSNRDKLFVLGTVKQINNLKQLIA
                     ELNVHV"
     gene            complement(37491..38759)
                     /locus_tag="PK252_06125"
     CDS             complement(37491..38759)
                     /locus_tag="PK252_06125"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOT14320.1"
                     /translation="MSEEFLQYIWKFRLFQNKVFTSQNGETVEILSPGEQNFNSGPDF
                     INTRIKINGVTWAGCSEVHKKASDWQRHNHHLDKAYDNTILHVVEEHDTDIKNSAGQT
                     IPFIELKYDSALLEKYKNLVSVSTNIPCKSCLPKIERIELQQWLTKLMVERLEKRTDE
                     ILQILNFTNNHWEEAFYIMLCRNLGFKVNAEPFEMLARSLPLQILAKHKNNLIQLEAL
                     LLGQAGLLSDKPADEYCSQLATEYNFLKKKYQLISLQKQVWKFMRLRPANFPTIRLAQ
                     LASLINNSSSLFSKLIEAEDIKQIEGLFNTSTSAYWENHYTLGKTSPASPKKLSKESI
                     DLLTINTIIPFLFVYGRERKILHLQDKAFQLAEAISAEKNSIIAAWKKYGAKVSNAFE
                     SQALLQLYNHYCTKQKCLHCTIGHWYLTKF"
     gene            complement(39245..39511)
                     /locus_tag="PK252_06130"
     CDS             complement(39245..39511)
                     /locus_tag="PK252_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14321.1"
                     /translation="MPETVVITDTSCLIALSKLDLLRSLDEIYQDIRITPEIESEFGD
                     PLPEFIKISEPQDNKYQKILETILDKGEASAISLAFEYDNVYLF"
     gene            complement(39504..39731)
                     /locus_tag="PK252_06135"
     CDS             complement(39504..39731)
                     /locus_tag="PK252_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOT14322.1"
                     /translation="MRAITLNIPDDINLKTLDLSIYLASKMYEDEIISSGQAAKMVGL
                     SKRSFIEIMGKYGVSVFSSSTDDLLSDIKNA"
     gene            39906..41822
                     /locus_tag="PK252_06140"
     CDS             39906..41822
                     /locus_tag="PK252_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT14323.1"
                     /translation="MMTFEELGLKPEILKALQELGFENPMPVQEKVIPLLLSKNTDIV
                     ALAQTGTGKTAAFGLPIVEQTDTKSKKTQALVLAPTRELCLQIADDLNDFSKYVDSLS
                     VVPVYGGASIEVQIKALRNGAQIIVATPGRLIDLINRGVAKLENVSKVVLDEADEMLN
                     MGFEDDLKTILAQIPENRNTLLFSATMPPAIASIAKNYMSNPLEVTIGQRNTGAENVK
                     HVCYTVHAKDKYQALKRIADYYPNIYGIVFCRTRKETQEVADKLMHDGYNADALHGDL
                     SQSQRDFVMNRFRLRQLNLLVATDVAARGIDVDSLTHVINYNLPDDVEVYTHRSGRTG
                     RAGKTGISIAIINLREKHIIKQIERIINKKFELQDIPSGRGICEKQLFHLIDKLERVE
                     VDEDEIAPFLTTTFKRLEWLDKEEIIKRFISLEFNRFLEYYKNMPDLEIPTEHSRREG
                     QRDSFREGKREGRREAGAPRTAEKGYSRLFLNLGKEDGLYPPDLIELLNQYSQGRRIK
                     VGRIELMKKFSFFEVPEQDAQFVVDSMKGASVSGRRIAVDWASGGENEGKQQSRGRDD
                     FKRSGERSGFKKFGERGNRGEHGDRKRFDKDRGEKNERNNSGNRSDRSEKKEKGSFRG
                     EEKKKYGRKKENRR"
     gene            42144..44063
                     /locus_tag="PK252_06145"
     CDS             42144..44063
                     /locus_tag="PK252_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT14324.1"
                     /translation="MKKYLFSVLTLICMSMQGQITMPKVFSNHMVLQRDINIPVWGGA
                     TPGTTVIAELNGVQVMGITDEAGKWMVHFPKFKAGGPYSLKVTEKGKKSYAFEFSDVM
                     IGDVWLASGQSNMEWQVQQAKDAGTEIKNANYPNIRFFSVPHDIALKVLSDTKSSSWQ
                     LCDTNNVKTFSAVAYYFAREINKDEHVAIGIVQSTWGGTPVEAWTSREILLSSSITRK
                     RILDNDSLTELHLKNEETNLKRFWEIVYNPQNGMDTIVPKENYVDKDWRTISMPSTFK
                     QWGMPPYEGIMWLRKTITLPESFAGKDLTIYLGHPEINYSLYFNGQNICKTIWNAALT
                     HYYTIPGKLVKAGNNLISVRMSLLWNAGGFNPPAVEMFITDGNEKVSIAGDWDTNIKL
                     EPAVPKIFNFQYYPTILFNAMLNPVIPYGLKGFLWYQGEANDSAPAHYRSLLPMMVAD
                     WRIRWQQGYLPFLIVQLPNYMKPCTEPQEKSNWAEMREAQQLATLQPNVGIACTIDIG
                     EENSIHPINKQDVGHRLALQAKKLVYGHDVIASGPVFTNMEINGNEITLNFTSTGAGL
                     VTKNNAALKGFAIAGENKKFVWADARIVGNTIVVSAKSIAKPVAVRYNWADNPAGNLY
                     NKEGLPALPFRTDCW"
     gene            complement(44566..44982)
                     /locus_tag="PK252_06150"
     CDS             complement(44566..44982)
                     /locus_tag="PK252_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOT14325.1"
                     /translation="MKKVFVDTNIVIDLLSRREPFFEEAAILFSLADKKQIELTVSSL
                     TIANTSYALLRQLDSINAKSILRKLRLIVNILPLDDKIIGLALNDNTFSDFEDELQYF
                     SAIENEQELIITRNLKDFKNSKLPAMTAKQFIETIE"
     gene            complement(44979..45230)
                     /locus_tag="PK252_06155"
     CDS             complement(44979..45230)
                     /locus_tag="PK252_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOT14326.1"
                     /translation="METKLTLRLNDNVIERAKIYAHSHKISLSKMIESYLDSITKQKV
                     ENKKISITPLVESLSGVIDLPADFDYRKEYRDYLEEKYK"
     gene            complement(45415..45816)
                     /locus_tag="PK252_06160"
     CDS             complement(45415..45816)
                     /locus_tag="PK252_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14327.1"
                     /translation="MENISKKICLHCQKPLEKGRSDRKFCDYYCRNAYNNQRTNESEK
                     IIKTINQILRKNRTILRNLNPTGHSTVRKEYLVMQGFNFNHFTHRYVANNKNEYWFCY
                     EYGYMFKALENEGDVEKVVIVNWQSYMAKPS"
     gene            45918..47357
                     /locus_tag="PK252_06165"
     CDS             45918..47357
                     /locus_tag="PK252_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT14328.1"
                     /translation="MNPKLPIVFANRFVHGSHKLIKFWFNHNPDVADMLEKLPWVRFS
                     FQYGCFYIIDDGVEINKTVEALKDKVLFNLKRVNTFISKPLTVVATGYNALHQPETVI
                     KKPVVKFLPVVSGGREVVLLTFEYNRELYMMLLKSGLVVWDKQLRGFVLPCDTDSIKN
                     IVRFFRGRVKVLFNSAMKVRDTELMKLLLEQGWASDESFVGCPLAYLDKLFLKEYSPN
                     TIRTYHAMFIRFLNHFKRYSMEQIDAMDAVQINAYHALLKASNKYSVSYINQSINAVR
                     FYYAEVLQRQVEMDGIDRPQRGFVLPKVLSEAEVEAIFDKVRNIKHRAMLMMMYAGGL
                     RISEVLQLKIKDIQGERKMIYIQGGKGNKDRYTVLSDKLLVLLREYYKVYRPKEYLFE
                     GQYGGAYSAESIRSVLKRAIEESNLNKRVTPHMLRHSFATHLLERGTDLRYIQEMLGH
                     ESTKTTEIYTHVSKKQISKIISPADFLKI"
BASE COUNT        14441 a         8289 c         9793 g        15922 t
ORIGIN      
        1 aacaaaaaaa agacaaaaga caagagcaaa tggaaaaaaa acaagaaaaa gagaaaaaag
       61 aacaagaaca atatgccaaa gctcaacagc accaacttga tattcaaagc aaaaaaactc
      121 gggagcgcat gctcaaaaac aagaaaatga cagatgacta ttataaaaag aaagcccgtc
      181 accgatgctg gagaaaattt gttacatttg ttcaacaaaa ttttccttaa gatatggacg
      241 cagattgtaa tttcatacca tttttagtgg ttaataaggc cgggattgat ggcggcacac
      301 ttttttatat tattatagcc gttattgtcc ttattagtgg atttattcag aaaaaaaatg
      361 caaaaaacaa ccaggagcct tcaaaacctg aacatgctcc cgatccttgg aaagaaatct
      421 tcgatttccc tgaaatggac gaaaaacctg agccagagta taaacctgaa cctgtaaaag
      481 ttgaagtaca aaaaaagcct gaggtaaaaa aagcccctga gccgataaac ccttttttgt
      541 cgcaagaaac cttagaatac ttaaaaacaa gagaaggaac cgtaacaaca tttgatgatg
      601 taatcaaaca gagcgaaata ggtagtgctt ttgaaagtga acactataaa ccaattgaaa
      661 ttgatttaaa aaaagcagtg atttattctg aaataatcaa ccggaaatac cattaagctg
      721 tttttggtaa aatattgatt attaatattt taaattttat ttgatttcta tttgcattta
      781 ctctttaaag agttgtatat ttgcaaaaaa gagtttcggg ttgccggaat tcttttttat
      841 tttatatgta tagtttataa taggaggttt tagcatgtca aaagtatcat acataacaca
      901 ggaagggctt caaaagctta aagctgaact cgaacatttg ataaatgttg agcgtcctgc
      961 aatctcacgt cagatagctg aagcccgcga taaaggcgat ttgtccgaaa atgcagaata
     1021 tgatgctgct aaagatgccc aaggtatgct tgaaatgcgt atttctaaac ttgagcagct
     1081 tcttgctaat tcaagggtta ttgatgaatc aaaaattgat acttctaagg ttcaaattct
     1141 aaacaaggta aaaataaaaa ataccaaaac taatgctgtt cttgaatatc agattgtttc
     1201 agaaacagaa gccaacctta aagaaggtaa aatagcggcc aatagcccta ttgctaaagc
     1261 tttgatgggc aaaaaagttg gcgatgttgc cgaggctatt atccctgccg ggaaagttat
     1321 gtttcaaatt cttgaaatat ctcgttaatt tataatcata tttgtttttg gaaaattttg
     1381 agtttgttgg ataccggttt ccggcagagt gggagaggca tgaagctaca tggttgacgt
     1441 ggccttatcg tgaagaatca tttcctgaaa acttgcatgt ggtatatgag cagtatgtct
     1501 gttttattgg gcaaatagca aaatcagaaa aggtccgcat caatattcct gatgaagggc
     1561 aaaaggctgt tttgtatagc tggctcgaaa agctcagttt aggcgacgag aatatccaat
     1621 gctttataca tccaagcaat gacgtatggt gtcgtgatca tggccctgca ttcattgtca
     1681 accctaaagc tgtatatcca aaggctattg ttgattggga atttaatgcc tggggaggga
     1741 aatacccttc caattttgac aatcagatac ctcagtttat agccagtaca ttttcattgc
     1801 cttatgtaag ccctggcatt gtcatggaag gaggctcggt cgatttcaat ggcgaaggcg
     1861 acttgcttac aactactgct tgtttgctca ataaaaaccg aaatccacat ttatcgcaaa
     1921 aacaaattga agagtatttg cgtaagtatt atggcgttca caatattata tggcttggag
     1981 acggtattgc tggcgatgat accgatggcc atgttgacga tattacccgt tttgttgatg
     2041 ctaataccat cattactgtt atcgagcatg ataaaagtga tgaaaattat gaaccattaa
     2101 aagaaaatgc ccggaaatta gcaaaagtta ggttagccaa tggaaaacaa cctgacattg
     2161 ttgaattgcc aatgccaaag cctttgtatt ataaggctga caggcttcct gcttcttatg
     2221 caaattttta ttttgccaat agcgctctga ttgtgcctgt ttttaattgt gatgcggatg
     2281 aaaaggtact tgaaatcctt tcaaatctta tcaaagacag gcctgtggtt ggaatcgact
     2341 cgtcaaatat agttcgcgga ctgggtagtt ttcattgtct cagtcaacaa gaacctttag
     2401 tatagtacat atatacaatt cattgtatgg gaagaaaaaa gtcattgcct gttattgaag
     2461 atgttgaaat tattgaatta gctgctgaag gcaatgccat agcaaaaata gatggtaagg
     2521 tattgtttgt gcctaaagcc attcccggcg atgttgttga tgttcaatta acccgaaaac
     2581 ggacttcctt tatggaaggt tatgttgtca attataagcg atattctcaa catcgtattg
     2641 aacctttttg ttctcacttt ggaacatgcg gggggtgtaa atggcaacat ttaccatata
     2701 ccgaacagct ccgctggaag caaaaacagg taacagataa tcttcagcgt atcggggggg
     2761 ggcaaataga aaaggttttc aatattatgc cttctgaaaa tacaaggttt tacaggaata
     2821 agctggaata tacattttcc aacaaccgtt ggttgcctgc tgaaatgatt aaacaacctg
     2881 attttgtacg cgaaccaggt gccggctttc acattcaggg catgtttgat aaagtgttgg
     2941 atttagatta ttgttatcat caacccgacc cgtccaatga gttccgtttg gctttgaaac
     3001 agtttctttt agataacaat ttaagctttt ttgatatcag ggagaaaaaa ggctttctca
     3061 gaaatcttgt tgtacgcaac actgttgccg gccaatggat gctaattgta atatttggtg
     3121 aagacaatcc gcagcagatt acaaaaacgc ttacctattt aaaagatagg ttttcagctt
     3181 tggattcatt gatgtttact gtgaatacca agctcaatga tacttttcat gatttagaca
     3241 taaaacttta ttatggaaag ccatacattg ttgagaaaat ggaagaccta cagtttaaga
     3301 ttggccctaa atctttcttt cagacaaata ctcaacaagc tgtcaatttg tactcaaaag
     3361 tcagagagtg ggcttcattg catagcgatg acattgtgta tgacctatat accggaacgg
     3421 gaaccattgc caacttttta gctccgcatg ctaaaagggt tgtaggtatt gaatatattg
     3481 cagatgccat tgaagatgca aaagaaaatt caaaattaaa taatattact aatacgttgt
     3541 tttttgccgg agatatcaaa gatttgctca atgattcttt ctttaatgaa cacggcaggc
     3601 cacgggttgt ggttacagac ccgcccaggg cagggatgca cccggatgta ataaatgcac
     3661 tgcttggggc cttgcctgaa acaatcgtgt atgttagctg taaccctgca acacaagcca
     3721 gggatataaa acttatggct gataaatata aagttgtttc tattcagcct gttgatatgt
     3781 ttcctcatac tcatcatgtt gaaaatattg cacttctgag tttgcgatga cagaaattga
     3841 tattagtgcc aaaagattgc tggaaaagct ggctaagaaa gtgggaaaaa ctatattaaa
     3901 tcatggtttg gttcagccag gagaccgaat tcttgttgca gtttcaggtg gaaaggattc
     3961 ctatgcctta ttgcacctgt tgaccgaaac attgcgacat gcacctttca aaccatacat
     4021 tgtagctata catgttgtta acaaagatat aggttaccag attgaccttg atttcatgaa
     4081 atcattttgc ctgtatctgg gcatcgactt gtatattgag gaaagtacag ttgatttaac
     4141 tgtaaaatcg aaaaaatccc cttgttttat ttgttcgtgg gagcgtcgga aagttatttt
     4201 tcaaaaagca agagaatttc agtgtaacaa aattgctttt gggcatcacc ttgatgatgc
     4261 tattcaaacc cttttaatga atatgatcta tcatggctca ataagttctt tgccgtattt
     4321 cgtcaaaatg tttcaaggag aaaggacttt ggtacgacct ttgctggatc ttgatgaaca
     4381 aattttgagt tctttggcaa aaatgatgaa ttatcctgtt gaggttaaac aatgcaaata
     4441 ctctgatact actaagagag atgcagtgaa aaaaataatc gaggaattaa acagcaaata
     4501 tcccctgaca ggtaaaaatc tttttaaatc gatgcaaaat tgctttcacg aatatttgcc
     4561 tgaacttaac caaaccttga ataaatcccc tgaaacttaa caatgatttg tatagacaaa
     4621 cgtaattatg tattttttaa acttacaaaa aatatactat gaacaggata caaccaaatt
     4681 ttgaaacaat ataccggatt ttcggattga tagttgttat tttgcttata attcttggct
     4741 tttctctcat gttttcaaag tattttcaat atattccttc aaattttcga ttttttatag
     4801 gtgtatttat aatcgcttat ggagctttta gattggtcaa tatcattata aaatttaaaa
     4861 ataaaaccaa tgaagaggat gaagagtaaa atatggatag ctggcttagt tgctttgctt
     4921 actgtttcgt gcaactatga ccccagggca ggcataaacg aagaaactcc tacccgcgga
     4981 aatatcaaaa ttggtgttga tgagtctttt tatttattgt ctgaagctca tcttgctgtt
     5041 tttcagtcaa tatatgaaaa tgcccatatc aaacctcttt ataagcctga gttggacgtt
     5101 cttaacgatt ttctcaatga ttccatcagg cttatggtta cttcccgaaa gcttacaacc
     5161 gaagagcttg agtactttaa gagcaggaat atttttcctc aaacagttac tattgcatac
     5221 gacggaatag catttattgt aaataaaact aataaagatt cccttatccg gtacaaccag
     5281 attcgggata tttttttggg caaaattgat aactggaata aaatcaaccc taaaaatacc
     5341 aatggggcta ttaaagtagt ttttgacaat ataaaatctt ctaacgttcg ttatctaaac
     5401 gagaaattta aagttggtag taaatttccc aaatattgtt actctgttaa taccaacgaa
     5461 gaagtaatta attatgttga aaaaaataaa aatgctattg gtgttttgag cgtaaattgg
     5521 attagcgata aagacgattc tataaccagg ggctttctta agcgaataaa agtagttggc
     5581 gttacgcctg aaaacgaccc cgaaggcccc gaatatacca aaccttatgc tggctttata
     5641 gctgaaaaat catatccttt tattcgtgag gtttacatga ttaaccggga atcattttca
     5701 ggacttggtt ctggttacct gcaatgggtt acagcagatc agggacagcg tattgtctta
     5761 aagatgggaa tggttcctgc tacaatgcca ataaggctta taaagacgaa atctaatttt
     5821 taaattgtta ttaatatgga ataatgtttt ttttcatcga ataaaaatta attttgagca
     5881 aattttttaa ctaacaaaaa ttatctagct atgaaaatat tctcttttcg tatttcaatg
     5941 tactggtgca tagccgcatt gtttttaagc ttaggttcgg cgttgttgca agcgcaggat
     6001 attaatcagg ctctcaaata tctgcaaagt gaaagatatg aagatgctaa tgcgattttt
     6061 caaaaattga tcgactctaa ccctactgaa gcaaatgctt gtttttatta cggtgagagt
     6121 attttgttaa acttccagaa tgatccatat tcaaatactt tagaggaagt cgtaaaagaa
     6181 tcggcaacat actttaatat gggcattaaa cgtgatagta caaatatgtt aaactacatc
     6241 ggactgggta tgctcaaact tcttcaacat aacgatacta ttggggccga tatatatttt
     6301 actaaagttg aaaatgtaat ccctaaaaaa gagaaaaaat ttaccgataa tgattttaat
     6361 ttattgataa aattggccat ggcccagttg tattctccta aacccagata taacaaagct
     6421 atagcatatc tcgaaagggc taaagccgcc tcggtggtta ttgcccaaaa aaccaaaaaa
     6481 gaagtcccat ctgtatatat tgctttagga gacatataca ataaaatgaa caatgcttct
     6541 ttagctgttg caaactataa caaagcagtt tatattgatg ataaactata tgaaccaatt
     6601 gttaaaattg gccgtttgta catgcgttct cgcaatttgc aggaagcacg taattatttt
     6661 gaccaggcaa aagaaataga ctctacttat gccccgcttt atcgtgctta tggcgaaatg
     6721 tatgcaatgg gtggtttggc caacctttca aaacaaaact tcaaaaaatt cctcgacctt
     6781 tcaggtaaca atattcctgc aaaaatccaa tatgtaaact ctttgttcaa agcaaaagat
     6841 tataaaacct gcgttgaaaa tattgaagaa atttttgcag tggacaaatc aagaaattat
     6901 ttgaaccgtt tggctgctta ttcgtgttat gaaatgagac ctgccgacta tgaaaaagca
     6961 aaaatgtata tcgaagaatt ttttaaaaat tcctctcccg agaaaattat ccctaaggat
     7021 tatacttatt atggaaggac tttgttgaag atgaaagata caaccttaat aatgaaagca
     7081 tttgaacagc ttcaaaaagc ttattctttc gatactacag attatgcttt gcttacagat
     7141 attgctgttg atgcttatta tgccaagcag tttaaacttg ctgttgaaac ttttaacaaa
     7201 aaaatagctt caggacaagc tgaaattagt gattatttta ctttaggcaa aacttattat
     7261 caaatggccc aaggtgaaaa agatacagct atccagcggg cagcttataa tgatgctatc
     7321 aaaactttta caactattac aaaacttgaa ccatccaacg ttgaagctta tttatggatt
     7381 gccaatacca gttttggact tgaccctgag tcaaagttag gtattgccaa accggcttat
     7441 gaaatggtta ttgaaaaagg cagtgctgac accattaaat atgcccctca attatttgct
     7501 gcatactcat acatgggttc atactacctt tttgctgcca aacctgatta tgctatggca
     7561 gagtctattt tctggaaaat ggtttggtta gaccctaaga aaaaagaatg gcaggtgaaa
     7621 gggtattatt ctctgggtgc tacatatacc agcagaaaaa attatgctgt agcccaaaca
     7681 tattataaga aagtgcttga aatagacccg aaaaacgagg cagcattgaa ggatttagaa
     7741 agcattaaca aagtgttaaa agcaaaacaa aatcaataat actaaacaag cattttggaa
     7801 aataggaaag aaaatgcttt atttgtgctc gaatttttag tgaaaattta attaataaca
     7861 actaatttaa tttatatgaa ggctttaaaa gaaattttta gttcagtgtt caacacatta
     7921 gtaattttag tgtgcttagc tattggtatt accatttaca tgaaagtgtt aggtaatcct
     7981 atcaacttta tggaaggtaa ccctaaaggc cacccacttc cagggaacta tcttgggatg
     8041 atgtataaag ggggccctat cgttcctgta cttatgaccg tagttttagt tcttcttacc
     8101 tttacttttg aacgttttat ttctttatca cgtgcttcag gacgtggtag tgttggtaaa
     8161 tttgttcgta acattcaaac tttattgaat gaaaacaaga ttgaagaagc tagtgctgct
     8221 tgcgacaaac aaagaggttc acttgctaat gttgttaaag ctggtttagt tcgttatcgc
     8281 gatcttcaaa atgatacaac attgccaaaa gatcagaaag tgttagcttt acaaaaagac
     8341 cttgaagaag ctacagctct cgaattacct atgttgtcaa aaaaccttgt aattctttct
     8401 actattgctt ctatctctgt gcttatcggt cttatcggta ctgtacttgg tatgatccgt
     8461 gcatttagtg ctcttgctac agctggtgcc ccagatgctt tggctcttgc tactggtatt
     8521 tctgaagctc ttattaatac tgcttttggt attagcggtt ctaccattgc tattttggtt
     8581 tacaactatt tctcttcaaa gattgatgct atgacataca aaattgatga atcaaacttt
     8641 agcgtagtac aaacatttgc tgctactttg aaataaaaat aaacaggttt gattttatca
     8701 attattaact taaaaaaagg atagttatgt caaaaataaa aatgacagtt cgtactccga
     8761 ggattgacat gactcctatg gttgacttgt tcatgcttct gttaacattt ttcatgttaa
     8821 cgacaacatt tcgtccttca gaagcggttc aggtcgatac accaaattct atatctgaaa
     8881 aaacagctcc ggataaaaac gttataacca tttttatatc gaaagacaat aaagtgtttt
     8941 ttaacgttga tgaaggaaca gatacatctc agcatattag aagacaagtg ttgaaatcca
     9001 taagtgagca ttttagaatt ccctttacgg aggagcaagt tgcaaagttt gaaaaacttg
     9061 catcttttgg aatgcctgtt aaaaatatcc ctgcatggat tgatgctgag caaaaacaaa
     9121 gagatgagat gcaggttggt atgccttatg atagtgtgga taaccaatta gctttgtggg
     9181 tattgtttgc ccgtaatgct aaccctaatg cagaagctgc tattaaaggc gatgctgaag
     9241 ctgattataa agttgttaaa agggtattgg atatccttca ggactataag attaataagt
     9301 ttaaccttac caccaacctc gaaaaagtgg aagttaagtt agaaaactaa aaaagtgaaa
     9361 taacatggca gaaattattc aagacgaagg tggtcataaa aaaggtaaac gcagggcaaa
     9421 aaaacactct actcatatag acatgacacc aatggtggat ttagcatgtc ttttgctcac
     9481 attttttatg cttaccactg catttagtaa acctaaggcg atggagattg tccttcccga
     9541 taaagtgaag gatgaaaagc cggatcctaa aaatgaatca aagattccta agtatcgtgc
     9601 tgttaccatc atgattgatg aaaatgataa agtttactat tatactggtt taatggaaaa
     9661 agaaccttat ccggaaatag tagaaacaga tttcagcaaa gatgggttgc gtaaattttt
     9721 acttggtcgt aacaagaaac tatacaaaca gattgatagt ttaaatacag cagttttaaa
     9781 aagtaaaaaa gctattagcc ctgatactgt tgttaagatg aaacgtaaac ttaggaaaga
     9841 agataaagct gggcctattg ttcttattaa agccactgaa aaagctaaat ataagaatat
     9901 ggttgatgtt attgatgaaa tggcaatatg cgaaattcct atttacgcta tcgttgatat
     9961 gacccctcag gaaaagaaaa tgctcgaagc taagaaaaag ggcacaagta ctactaaaca
    10021 ataaattata agagtatgag tgaaaacaat ataaatatta gcaatcaggc agaattttta
    10081 gaacttgacg aaattgtatt ttcaaagcgt aacaaagaat atggagctta tgcattacgt
    10141 aagagataca aaaaagtcct tctgattgca ttttcgggtg cgtttttaat tattggcact
    10201 gttgtagctg tacctcttat tgaagcttac cgggctaaaa gcaaagcttt gaaacgcatt
    10261 gacaaaaatg ttagtttgga gatggaaaaa cttaatcagg aggatgcacc tccaccacct
    10321 ccaccacctc caccaccagc tgcgcttgaa caacaagtta agtttagggc tccggttgtt
    10381 gtagatactg taaaagaaga agttgcactt gctacggttg aagaacaagc tgaaatagta
    10441 aatgaaactc ctcctgaaga aattgtagta gaagagaaaa agaaagatga agtcattgaa
    10501 gccgaagaac ctgcgtttgt tattgttgaa gaaaatgcat cgttccaggg tggagatatt
    10561 aactctttcc gtatctgggt acaacaaaat attgtattcc ctactgctgc agcagaagct
    10621 ggtatttcag gaaaagtaat tgttcagttt gcagttaact ctcgtggcca gattgtagat
    10681 gcaaaagtcc ttcgtggtgt ccaccctgaa cttgataaag aagcgctacg ctgtattatg
    10741 ggttcaccta aatggactcc tggtaaacaa ggcggtaaag ctgttaaaca gcaatttgta
    10801 attcctattg tatttacact tcaatagtga taatataact caaaaagaaa gctgctttca
    10861 acagaaggca gctttcttta tttttatact actgaagtat cgtatagtga tagtgaacat
    10921 tgattgaaat agcagggcaa gcgcatttaa aaaacgatat atatcatatt atcaataaga
    10981 tgtgtgtgtt gcttttaaaa cctcccaact ctctttaaga agtagggcta acattgcagt
    11041 tgtttgcata gcccgttata gtcccttttc aagtgggtgc ggattatttg tattttaatg
    11101 cttttgccct gattgaaata ccctattagt ttttttgtaa tgcaaagtaa tggttattta
    11161 tactatttca aatcaacgag caaaactttg catgaaataa aaaaagcacg ctcattggcg
    11221 tgctttactc tttatagctt attaattttt actttttgct atcaataggg agaatggttt
    11281 tgccataaac ttcattaata atctgtgcaa ggcatgtgta aatagccaat aaaccacaaa
    11341 taataccttc atagcctgca acggttttta tttctttgat acctgtaaag tcaccaatag
    11401 cgaggagtaa aaacagcact acaagggtcc caaacacaag ttgcaatgcc ctgtttaatt
    11461 taagtgtgcc aatgaacatc cctaaggtga aaattcccca aatcaaaagg aaccaaccca
    11521 tagatgtttc atctgatttt aaatcagctc caaagcttgt tcgtgggatt aaccagatag
    11581 caaccagcgc tatccagaaa aaaccataag ctgtaaatgc tgttgctcca aaagtattgt
    11641 ttttcttcca ctcctgaata cctgctatca cttgcgttaa acctccaaca aacagtccca
    11701 tgcccataat catagagtta agatcataaa agcctgcatt atgaatgttt aaaagaagtg
    11761 tggtcattcc aaatcctaat aacccaagag gtgctggatt tgctgttgtg tcttttacaa
    11821 ttgttacgtt ttctgccatt ttcattacat tttattttgt tttcggtgcg caaaaataag
    11881 ataataaatg aaataacctt attatgttaa tttttaataa aatagttaaa ttagccttat
    11941 gaacaatatt taaaaccgca taaaacacaa agattacctc aaaaggcttc aaaaaggcac
    12001 ttcaattttg ctgtttctgt agtagaatat gataggcccg tacaatcctg ttaagagcct
    12061 gtttcagcaa aataaaaggc gtatgacttt tttccagaac cagaatatgg ttatccccaa
    12121 atttttatta atccgtcaat aagattattt gtagcttcat taataatcat tcgttctggt
    12181 ttggcttcaa accactttat agtttctttt atcccttctg aaaatggaat aacaacttta
    12241 tattcaggaa caaattgttt gatttttgta ttgtcaaaaa tagcacaatg cgatttgtcg
    12301 ccccaaagcg aaccaaattc cgatggatag cctgttttgt ctgcatattg acagatttgt
    12361 tttgtgggta tgtggacaat attggcttta acaccagcag cctcagcaag agtttcatat
    12421 atttggttcc aacttaatat ctcatcggat gtaatatgaa aagcctcacc tatggcgtga
    12481 atatttccta aaaggcctat gaaacctcgg gcaaaatctt tggcatgagt aagagtccat
    12541 aatgatgtgc catcgccttg gactactacc gggagacctt ttttcatcct gtaaacagtg
    12601 gtgtactctg tccagcctcc taaagtaatg ggaattactg tattatacgt atgagatggc
    12661 cttacaatgg taatgggaaa acccttagtt ctgtaaaggt gcattaattg ttcttcacac
    12721 gcaattttat ttcgagaata ttcccaaaaa gggttaacta aaggtgtaga ttcagttata
    12781 atgtggttta caggtggctt ttgataagca gaggctgagc tgataaagat atattgtttg
    12841 gtactattag caaaaagttc aaaatcgcgg gttatatcat caactgtaaa tgcaacccag
    12901 ttgacaacaa catcccattt ttctgtggaa agaagctgct ttgttttaat tacatcatga
    12961 atatctgtat gcatttgctt tactccttca atactatctc tattgccccg gttaaggagg
    13021 taaaggttaa ttccttttga aacagccatc ttgcttacgg ccgagcttat gttacctgtt
    13081 cctccaataa ataatacttt catacgctgt ttttatcaaa aatacaatac aaaagacaaa
    13141 ctcctgatca atatcgatga tttaaaataa aggataaaaa tgttttgtgt ttttaatatt
    13201 tatatcaaat gtaatatact gatttgcaat gtattaatga tgtttaactt gttgataatt
    13261 ttaagtattt tttaagtatt ttagtttaat ttaatgtgaa aaaactcgaa atttttagta
    13321 tatttcggaa tgtaagtttt atcaaaccta aaatagtaaa actatgacaa taacatttaa
    13381 cgaacttagg aaaatcaaag acagcctacc ttctggcagt atgaaaaaga tagctgataa
    13441 gcttaatatc gatgaagata ctgtaaggaa ttacttcgga ggtacccatt acaaaaatgg
    13501 agaaggaaaa acagtaggtg tacatattga acaaggtccg gatgggggca ttgttacctt
    13561 tgatgatgaa acaattttat cagtagcccg tgaaatgctt gcagaaagtg cgcatcacta
    13621 aattataaac ccggtattaa gccgggtttt ttatttacaa ttcatccaaa gtagtttatc
    13681 tgcctggagg ctgcctaagc tcttggcttt tttccaatct tcgcaggctt gggaaaattg
    13741 ttttattgaa taataaatat tcccgcgttc gagcaatgct gaaatattat ggggttgttt
    13801 attcaataca gtatttaagt cctgaagtgc gttattggtc aattgtgcct gagcgtaaca
    13861 tttagccctt aaaatataat aatcggtatt gttatatccg gctttaaata gtgtattaag
    13921 atattccagg ctcttaatat atttattgct atgaaatgca gcttgtgccc ctatgtaaag
    13981 tcccttttcg ttttgaggca ataggtatat ataaatggct atttcttcca ggctttgttc
    14041 atattgttgt tggagcaaaa aacaatacgc atttttacaa attgcttcaa agtttagcag
    14101 gtctaagcgt ttagcttctt tatagtcttt tgaggcctgt acatattgtt ttagcttaat
    14161 ttttacatca gcccgaagtt caaaatactt gcttcggtgt ttgtttatcc ggatagcttc
    14221 atttatatca gaaagggctg cattatagtt tcccaaggcc agataagaac atgcccgcaa
    14281 ataatacatt tcgtggctta ttttttgatt attgatggca aagttgctta cggtgttaat
    14341 gacttccata taattgccag aatggtataa gaaaagcaat tcgccaatta gttcgtcatt
    14401 tttgttgtac caattttgtt cccaaagcgt tttccattgt tctgtttttt caagaaatga
    14461 gaaagctttt tccattttaa tgtcagaggg cttgacctga tattcggaag ataagttttg
    14521 ttttaagtat ttaatggtgt tcagggtgtc gcgttcggaa gcgaaaacta aggccagata
    14581 aaaggatgca gaattttttc tgagggtatt ggcttttaat aatggcaatt tagcatcttt
    14641 ttttaatgaa tataaaaaca taccttgcct cagatagaca aggtaattat taggtgtttc
    14701 ttctatggct ttgctgaaat aaaagtttgc agaatcctct gtgttttgct ggctataagc
    14761 aacggccttt tgcaaacttg tttgagccgg gaggtataga cttgaaaaaa gtaagatgaa
    14821 ggctaataaa cgcgtcatgt ttagcttttt acaacgcgtg gaaatttttg ttcctgcaaa
    14881 acaaggtctg ccaatgccag gcatgtcatg gcttcaacaa ccacgggaat acgcagggct
    14941 atacaagtgt cgtgacgccc ttcaacactg aggtctgtca tttgaccagt tttcatgttc
    15001 atggtgtgct gtgtggcatg aatgctgctt gttggcttta cggctacttt aaatacaagg
    15061 tcgttgccgt ttgaaatacc accattgatg cctccggcgt aatttgttgc tgtttttcct
    15121 gaaacagtaa taaacttgtc gttgtgttct gtacctttca tgcgggcagc tgcaaaacct
    15181 gaaccaaatt caagaccttt gattgccggg attgaataga tgtaatgcgc caaaacggct
    15241 ttaatattgc caaagaaagg ttcgcctaat ccgattggca tattggtagc aatagtttca
    15301 acgattcctc cgatggaatc ttgctctgca atagcttttt caactgcctt ttcaatgtct
    15361 gttgttcctc ctgcctcaat aagagtagat tttacaatga taggagcaat gatttttttt
    15421 gcaataacgc ctgctgcaac tataccaagt gttatacggc cagaaaaatg gcctcctccg
    15481 cgatagtctt catatccgcc aaacttttta tgggcaacaa agtcggcatg gccagggcga
    15541 ggggtttcta aaagaaacga atagtctttt gaacgggtat tggtattctc aaaaaggatt
    15601 aaaatagggg cgccggtagt atgattttca aatactccgc tcatgatgcg tggaatatca
    15661 gcttcctggc ggggagttgt acctttggct ccacttttac gacgacttaa gtcggcttca
    15721 agttcttccc gggtaattgg tattccggct ggaacgccat ctattactat tcctacacta
    15781 ttgccgtgag attcgccaaa tatactgagt ttgaagagag ttcctatggt attcatgttt
    15841 ctatattaat ttaccgttga aaattatgaa tttttgatga atgagtatag ttcagccaac
    15901 cttttatatt gaatctggcc gggagccgta gcgctttgct cgtcaaaagc agcaaaggta
    15961 aaaggagaac caagcaatgg ggctgctatc cttgatattt tgcctttttc gcccatgcct
    16021 aatgatacta caggcttttc agagtcatat aaagatagaa gagcagcatt gtcggcatcg
    16081 gtgtttacca tgcaagccag cttagcaata tctgcaccca tatcaaagca ttgctgaaca
    16141 ataatctcta attgttctct tgtcggagtt tttttgaaat cgtggaaaga gatgataagc
    16201 ttacaatttt tattttgtat ggcttgtttt acggcatgtt tgaaagataa atcagattcg
    16261 tattcgacat cgacaaaggc tgcaccgctt tcaatagctc ggataaggag ttttaggcgt
    16321 ttttcgtcag tgatattgtc cgggcggcag gtagctatca ggtttttatg agacgaaaaa
    16381 accctttcga cttgctgttc gttatagcct gccaaatcta tcctgatttc ggccatttca
    16441 aggcttttta aagcttcaat acattggtca acatctgtaa tgttaagcga gacgcatatc
    16501 atgaattatt tcctccaatt ttttatattc tacattttcg atgatagcat ggccaatact
    16561 gtcaagaagt acaagatgca aactggctcc atcgcgcttt ttgtcttttt tcatggcgtt
    16621 aaacaaggca ttgctatctg catccagata tgtgggcaat aagaagtttt ctgtaagctt
    16681 tttaagcctc agtgcttcgt tttgcgaaag atgaccaagc aggaccgaag cagcagcagc
    16741 catgttcatg ccaatagcaa cagctttgcc gtgtggcatc atggtaagct tttcaatagc
    16801 atgaccaaag gtgtggccaa aattgagttt tctgcgttcg cctttttccc gctcatcctg
    16861 tgacacgaca ttggctttta tttctactga atcctttata caacggtgta tggcgtcttt
    16921 gtcatatttt aaaatggcat tataattgtt ctcgagaaat tcaaacaggg ctttgtcttt
    16981 aatggcacca tgttttatca cttcggccat gcctccaata aattcttccc tgtcaagggt
    17041 tttgagcatt tcaatatcgc atatgacaaa ctgaggttga ttgaaaacgc ccaccatatt
    17101 tttgaatccg ccataattga ccccgttttt gcctccaacg cttgcatcaa ccatagatag
    17161 gagagaggtt gtgacaaaac caaaatttaa ccccctcata taaattgatg ctgcaaaacc
    17221 agtaatgtct gaaacaatgc cgccacctat acctacaatg aaggttgagc ggtcagcctc
    17281 aaactctacc aatttgccca tgataaggtc aatggttgca agggttttat tattttcccc
    17341 ctgagggata acgataacat caaagccttc aattgctttg gcataaagct ttgcaatgtt
    17401 atcatctgta atgatgatgg ttttctggtt ggcagggatg tattttgcaa gattataaac
    17461 cgattcgcca atcaggatgt tagaaacagc gcctgctgta tttaatacaa ctttctccat
    17521 gctatttatt accattttta ggctcattca tcacttttgt ctggtggtcg attgattcct
    17581 cgtgtataga acggaaaatg gtttcaataa attgggcact caagcctttt tttgcccctt
    17641 cttcaagacg cttggtaatg attttatccc aacggctggt ttgaaggatg gcaatgttgt
    17701 tttctttttt gcatacacca atttgttcgg ctactttcat acgtttttcg agcaaatcaa
    17761 caagttggtc atcgagtttg tcaatttggg cgcggagttc ttcaagggta actaaagagt
    17821 tgttgtcggc atattctttg cgcaatacca gtttactcaa caatacactt aaatcagctg
    17881 gtgtaagctg ttgtttagca tcactcatgg caacatccgg gttgatatgc gattcgataa
    17941 tgatgccatc gtaattcagg tccattgatt tttggcatat ttcgcccagg tattgtctgt
    18001 ttccacagat atgacttggg tcaacaaaca ttgggatgtt agggatgcga cggcgcagct
    18061 cgattgggat ttgccagttt gggtcgttcc gataaacgag cttttcgtga gaagagaagc
    18121 ctctatgtcc tgccgcaaga cgggttacac cagcatgata aaagcgttca atagccccaa
    18181 tccataattc aaggtctggg tttacagggt ttttaattag taccggaatg tcaacacctc
    18241 gcagggcgtc tgcaatttct tgcactgcaa aagggtttac agaggtacgg gctccaatcc
    18301 ataagatgtc gatgcccgct tttaatgctt catacacgtg tcttacgtta gctacttcag
    18361 tagcggtaag catacctgta gcttgtttta cgcttttcat ccaaggtaaa gctgcagagc
    18421 cgtatccttc gaatgagccc ggacgtgttc ttggtttcca aatgcctgcc ctgaatattt
    18481 tgattccctg ggctgcaagt tgagtagctg tttctaccat ttgttgctct gattcggcac
    18541 tacaaggccc tgcaataatc attggcctgg ttgaaggtac attttcgaat gtgaatggct
    18601 gtaattttaa ttgagattcc atattattta cttctttttt ttctgatgaa attgctgtag
    18661 tattcattgc tgtaaagttt atttaattat tattccataa cagttttgcc ttttgcatat
    18721 tcgcccatga tgtctaatct tgaggtaaaa ggcctgatag cgtctaaagc ctgtctgtac
    18781 attacgtaat cattgaatgt aacatctaca taaaattggt attcccagtc cttcccaatg
    18841 atgggcattg attgaatttt ggtaaggttt atcccataat aagaaagtat agaaagaact
    18901 ttagaaaggc ttcctgtttg gtgttcaagg acaaatgtaa tggatgcttt atcaatgtgt
    18961 ttgtcttgtt tgtgatatcc aattttttca gaaaggataa ggaaacgtgt atagttcatt
    19021 ttgttggttt caatgccttc tgccaaaatg tttagcccat atttgtcggc agccagtttg
    19081 gaacatatag cccctgtatt tttaagcttt ttatctgcta tatctttagc gctaagagct
    19141 gtgtcaatac tgtcaagtac tttaatatta ggataattgt caaaaaacat ctgacactgc
    19201 aatatggcaa tagggtggga gtacacttcg gtaatatctt caatttttac acccggtaat
    19261 gctaccaggt tttgttttat tcgcaggtat atttcgccaa taattttcat atttgaatct
    19321 tgcagcaagt tgtagtttgg tagaatgcta cctgccaaag agttttcaat agccataata
    19381 ccaaggtctg catggttgtt ttttaaagat ttaaataggt ctttaaatgt attggctgtt
    19441 actatttcaa tgttttcatt ttcaaaataa tgtttggcgg caatttcgtg aaatgctccg
    19501 tatccgcctt gtatggcaac tctgcttgtt tgtttggatg ttttaggtgt cataattttt
    19561 tgttttataa aaaaaaagtc ccggtttttg accgggactt gctattgttt tttgttttgt
    19621 ttatgcaata gaatattggc cccggtgttt ccgcagaaaa aagaaaaaaa agtaaaaata
    19681 attatttgaa acccaagacc gcatttgctt ttcttttatg tatttgttgt tgacaaaagt
    19741 aaaaattatt tagaataaaa ccaaataatt gataaaatat cttcttttat tactaaatgt
    19801 cttaaatttt gatacccata aaaagtacgt catctacttg ttcgagctga tctttccata
    19861 gattaaaagt gtttttgaca tgaaggtatt gttcttccat tggcaattca tgaatgtccc
    19921 gcagcatatt acgcaggcgg atcattttaa attttttacc cattggccct ccaaactggt
    19981 cggggtaccc atcagaaaac atataaatga tgtctccttt ttggagttga aaagtttggt
    20041 cttcaaaatc tttgccttgg tcgccaaatt cagtacctcc gatggagttt ttgcttccct
    20101 gaacgtaaac ctgctctccg ttttgataga gaataacagg tcgcatagca gaagctatgc
    20161 gtactacata agattcaaca tcaatttcgc aggcaataat atccattcca tcgcttgatt
    20221 tttcggcatc aaaattttgg ttaagggtat tggccatttc ttcatcgagc ttaaggagga
    20281 tatcagaagg tttgactacg tcagggcggg tacaaatatc tttaataagg gttgtcccaa
    20341 tcatagacat gagtgctccc gggacgccgt gcccggtaga gtcgccgcaa acaatagaaa
    20401 acttggtatc tgttactttg tcaaaccagt aaaagtcgcc actgacgatg tctcttggtt
    20461 gatagaatat aaaacaaccc gaaaagctat cctggagttt tttgatagag ggcaatatac
    20521 tagtttggat acgttgagca taagtgatac tgtctgtaat atcccggttt ttaatttcaa
    20581 tttcttcttt ttgttcaaca acttcacggg tacgctcatc taaaagtttt tgcaggtgtt
    20641 tttgaaaggc tatctgcttt tgttctctat atttaataat ggcataaacg gcagccacta
    20701 atgatattat agagagcaag ataaaccacc attgtttcca aaaaggagct ttgattttta
    20761 ccgtgacaga aaaaggtatt tcgttagcaa tgccttctgc attgtaggct ttaactaaga
    20821 atgtaaaatt accatcgtct acgcgtggat aaagggcaaa agtattggaa cttatttcgg
    20881 accattcatt ttcccagcct tccagtttgt attgatattt aactcctgcc gggttggtaa
    20941 aattcagtcc gacaaagtca aaacggatac ggtatttgcc ataaggcaat acaataggtt
    21001 tagaaaaatc tatttcttta tctccaattt taacagaaac caagtcaaca ttagggggta
    21061 aaagcttttg tttgttgctc gaatagttgt attgaacaat tccatctgta gtcccaaagc
    21121 gcaaaacacc ttcgtctgag gcattagcag catttaggtt gcagtcgccc cttattccaa
    21181 gttctattcc ataggttaat actttaaatg tattgctgtt tatacggctt aggcccatac
    21241 gatgtcctgt ccaaaggtta ccatcggggt ctgagataat gaagtatcca tagtcagatt
    21301 ttagtccttc gttttcggat aggtgaatga cagaatcttt tctaaagcag aaaacgccat
    21361 ctccataagt cgaagcccaa aggttgcctt tcttgtccat tgcaatgcct gtaaattcaa
    21421 tttcaggaat accttccatt ttaaaatgtt ttttgttgtc aagactaacc aaaccgttac
    21481 ttttggaggc aacccaaatg tttccgtctg gagaaataaa caggctgttg attttattat
    21541 gggggaggcc gttgtccata gagtattttt ctgtgcgtcc tgtgttttgg tctattttaa
    21601 ttattcctcc gtttgtggct atccaaagag tgtttttatt acaagtaaga tagttgattt
    21661 tattttcaac agaattgttg gaacgaaaaa cctttgatat ttgtttggtt tgaaaattta
    21721 aaagatacac gccatttttt tcagttccaa agtaaagttg gttattttct atagggagaa
    21781 tagcggtgat attgtctgcc ggcaatccgt ttttagtagt caatgtgaaa atttgtttgt
    21841 tttttgcaga cataaacgat atttcctgtc tgctgccaaa aaagatcccg tcttttgtag
    21901 aggctatgct caggatgttg ttgttgattt gttctttaga aaagttgtaa aaaacaaaag
    21961 attcatttat gatggcactc aatccgttgg aataagtgcc tatccataag tctttttcac
    22021 ggtcaaaata aatggatttg atgtaattgt ctcccagtcc gttgctttcg ttatagtttt
    22081 cggtaatttc aaacgtattg tttttgttgg caagtttata tatgccattt ccaaaggtag
    22141 caagccagat gttattgttg tcgtcaattt gaattgattg aatattggca agttttaact
    22201 tttggtcatt ggaaagtttt tgagcgttcg ttttttttga attgtattga agaaggctta
    22261 tgccttcatc ttctgttcca ataagatatt tgtcctggcc atagctagct atacattgaa
    22321 ttttggtttg ggctatgcat ttaatattat ttctaaaaat aaactctgac ttggctgcat
    22381 cgagttttgc ttcccaaagg ccggatgaag tgccaaccaa aaaatgattg ttaccgatat
    22441 tgataatact gtaaataagt ttgtcattgg tgcctggcag ggtctctgtt ttttcggggg
    22501 taataaggat aatgccgttg ttttgggtac aagccaaaat attgccatca ttgtcttttg
    22561 tcaggcaggt gatggtactt ggggattgag ggttgctgta tttttttaca aactttcttt
    22621 ttgaatataa ataaaggcta ccatcgtcaa agccaaacca tagagagtct ttagaagatt
    22681 gaaaattagc tgaagcgact gtgttgggga gtgtatcacc cataaaagta ttggtaaatg
    22741 ttgtcccgtc aaatcggtta aggccaactc cagtgcatag ccaaatgaag cctgttttgt
    22801 cctggatgat tgaatatacc gaagggtgag aaagcccttg ttcgatatta aaacgcttta
    22861 tgtttatact ttgcgagaag ccttgtgcta taagtaatgc tataaagcaa agcgataaaa
    22921 taaaagagcg tgtcctcata atagtgttat attattgttt gtaatacttt ttaagtaata
    22981 aacacgataa agttacaaac attcatgaaa ggaacaaagt cctattcggt tttctgctta
    23041 accccaattg aatatttata ttttggtact ccgccaatag gaattgtgta gaacggcaat
    23101 agctcgccat attggtttct gacgtttatt actacattgt tgtttttgac tggagcattt
    23161 tttcttttta tcatttggat atctaaagaa gtgttttttt cttcctggat aattttaaac
    23221 tcggtagtta cccaattgtc ttgtcctgaa acagcttcca tctggccttt acgggctaca
    23281 gatacaatgc ggataaagcc atcatcatcc cagtcgaagt tgctgatttt taccgagata
    23341 gttttgtcat ctacataagg ataaatatgc gaaacctgtt tagggtcatc ccacatcctg
    23401 aaacaataat cgtaggcaaa ggcatttgcg ccttcaattg gtacattatc ggtaggggag
    23461 gtagagaggt aaaatttata tttgtttccg tcatcgccac taatgccttc tgctataact
    23521 ttcagaacgt tgccgccaaa tttttgtacg tattcacctt ctgtagggtt aaaagggcca
    23581 aaagtgtacc atgttttatc gtattttgag gtattcccaa atgttttagt agcttttaga
    23641 ttgccactct tataatttcc agaagtaggg tttgagcttt gagctgcttc ttccgaccag
    23701 cattttttgc caccatacac tgaaaatgta gtttctgtat caaatacgcc gtttaattcg
    23761 tcatcatctc cgctggcttc ggggtcaaaa acacggatat aaacaggttt tttaaatttt
    23821 tcagggatta aaaagaaaaa tatttgtaca tggtcattgt ctccccatga ggtttcggca
    23881 tttttgccaa atgtaataag acttgcaata ttttcgtcat cagcaggggc agcctggcta
    23941 aaacaagcta taggtaaata actggcaatg agcaatataa ataggtgttt ttttattaga
    24001 gtgttcattt gtgtaataat ttatacaaaa gtaacaaatc gttgtttaat gttgtttaat
    24061 agaaatactt ttgaatacac aatatttttt tatgccggtg cgggtttgtg tactttctaa
    24121 cataagtttt acatcataaa ggccttcttc atagaaaaca tgtgatatgc tttcgccaaa
    24181 gcttttggta ccatcgccaa aatcccaata atatttagca attttaaagt ttttgaggtt
    24241 gctttttttg gcatcaaaag taatggtttc tccaatagtt gccatatccg gggctgtaat
    24301 gtagggttgt tcaatttctt ctacattaaa atcatattcg gcctggttca tcataatttc
    24361 attggtgaca gagtctataa cattgagttg gatacggtat tttccaggtt ttgcaaagca
    24421 atgttctgct tcaagccctc tgatttttgt cccatcgccc agatcccatt catatttcat
    24481 tgttcctttt acgtcttctt cactggtgcc attttcaaaa aaaacatagc aaaagttgtt
    24541 tttcttttgt tgggtacagt tttcaaattc tgaaatggct gattggacgc gaaagatgtc
    24601 atcgtttttg ttacggcttg aggcaaaaaa gcctgtttgg agatatggat ccaaaataag
    24661 cccaaaatca tcagctgttg acgaaaatgg ttctttcata ttttcaggtt ttatccattg
    24721 tccgttcatt tttagtgtaa agaatatgtc atatccacct cgtgtattcc agcctttgga
    24781 agcaaaaaat aagcgaccag actgatgaat aaaagggaaa gcttcatttt tgtcagtgtt
    24841 aacgttgggg ccacaatttt caggtttaga ccagcttcca ttgttcaggt gtgtaacata
    24901 aatatcgtat ccgccaatac ctccgggcat attagaggca aaatacatag tagtgccatc
    24961 agcagtaatg gttgggtgca ttacgttgta ttcggtgcta ttatattcaa aggctgtgat
    25021 atttacccat tcatcttttt taagttcggc aaagaaaatt cccaatgtat ttcctttttt
    25081 gttgtagttg ccaaattttt ttgtggcatc aaggtttcgg gtaaagacaa tacttttatc
    25141 actgttgaac aaacaacagg ggccttcgtt gaaaatagtg ttaaggttga gcctttcaat
    25201 gcttttatag tctttgaaat cttttgttag ttttacctgg tagaggtcaa aaggttgaga
    25261 agagttttcg gcggtatcag agtatgaaaa ccagaagctg tgttttctgt cggtacaata
    25321 aataatgccg tctttggcaa aagcgggtgc aaattcgttt tgtttgttag aacagaacga
    25381 aagcttttca acggtgtatt gacgttgaga gaagcccagg ttcagaggta taatatctgc
    25441 caggaataaa aaaacgccat atattatgat tttcttcatt tttattttct ttcaagcatt
    25501 taaaataagc gggggtttgc agctttaatt ttatacaaaa attcataccg gaccgaaaat
    25561 tcgaatgaat aatagttgta tgtattagtt gatggtgttg agtattcata cgtacatcct
    25621 attcgtaact gatggtttag ctgtacttca acaatcccgg ccatagcttc tcccgttcta
    25681 taaagggccc cgaagctaag catatcgtta agtgtaataa tattaaggtt tatatcaaac
    25741 tgttcttttg ctgtcatgtt atatttgagt aaggtagaag gtttaattct taaattgggc
    25801 gaaatgttta gcaaaaagcc tgctgttaat aaaaagttat agttgtttat gtcatgaaaa
    25861 atttcaaaac catcatgtga gtttttttct ttataactta aaaaatatgg gatagaaagt
    25921 cctgtaaaaa aggtatttga atagtaatat attccggctc caaggtttgg cagcagcagc
    25981 gttgtgttgt tgttttcgaa ggccgggtcg ccgctttcgt tagtataaag gttactccag
    26041 ccataattgc cataattaag ccctgcttta agcccaaagg ctaatttgcc tgggcctgcg
    26101 gcaacccgat atgcgtaagt agcataaata tgagtattct gtatagggcc agctttttca
    26161 ttcaatgcca taagtccaat gccgatattt tcgtttttca acggcatgtg ggctgtgaaa
    26221 ctttggtagc ttggagctcc tttaaaacct atcatttgag agcgatgcat tgcattaaca
    26281 ctaagcacat ccctgctacc tgtatatgcc gggtttagaa gcaccccgtt ggacaaatat
    26341 tggctaaaaa gcgggtaata ctgggcattt gcatttattg caacgataga caatatagat
    26401 aatatggcaa gtttcttttt catcatttgc ggattacaat gtatccttta taagtgcggc
    26461 caatgacatt gagaatatag taataagtat cagatggtag ttctttgcca tcttgggtgc
    26521 cgttccaatt gtttttataa tctggttggt tatatatttg ttttccccat cggtttatga
    26581 tagtgaggtt tgcgtctttg acattctcaa ggccttttat ttcaaataaa tcgtttattt
    26641 tgtcaccatt aggtgaaaat ccttccggga ttttgaggtc tgtagcttct acaatcaaag
    26701 tgtcacttgt tgcttgacaa acgccattag ttaccaccca aataaattgg tttttcccaa
    26761 atttcaggtt ttctacatac gtttcaggat cggagttgtt ttcaatgatt atactgttat
    26821 cggggcttat ccactttcca acgcccaatt gtggcaaata tgctttcagg tgtgttttaa
    26881 atagatatgg gccagattgg ttttcaccaa ttgaaggatc taaaggaggc tggaaaaagg
    26941 taacctgaat gttttttttg tcaatacagc ctccgttgtt agcttcccaa gtaacatttt
    27001 gagggccaaa ggcagatgtt ttgatagata ctttatttgc tgtatcggca ggatagaaag
    27061 tagcaggcac ttctaatgac catttgcctt ttccaatagt ttttgttgct atcagggtta
    27121 tactgtctcc gcagacagca gtatcgatcc cggcaaaaac aacaggtact tcaaatacat
    27181 gtttggtagc tgttcctgac atatctattg ccaggcattt gttgttgtct tgtactgagg
    27241 taattaaata attgtaaacg cctgctatta atggggtttc agaaaaagta tttaagccgg
    27301 tagtattaaa ttctttttct ttagtttcaa atccattggt gtatttaatt tttataggag
    27361 ctttgccggt aagcctgatt gatagatcaa ttttgttgcc gagacaaaga ctgtcatcaa
    27421 attgggtcag ggtactggta ggaagctcaa aaattttgat caagccaatg gatgaagtgt
    27481 ctttgcagca atcgttaaga cctgaaaata ctatgcgcct gaaataaata gcagaatcaa
    27541 tagccggagg gttgtattgc atcactgaat agttgccggg gcagtttttc catggtttat
    27601 tttcttcatg gcattgccaa aggtatttgt atgtgccatc tcctcctttg ggtgacaggg
    27661 cattgaattt ttctaaatcg gtccctttgc agattgtagt gtctgttccg gaaatgttat
    27721 tggatattaa actaagcact ttgactatta aagtatcgct atagctttgg caaattcccg
    27781 aaattacctt tcttctgatt tgtacagtat ctttaaatga tgggggggtg aagtctttta
    27841 aagtgtcttt ttcgttatta acgctgaatg gtccgttttc tttaatttcc cagaagtact
    27901 tgtaaggccc gtctccgaaa agaggcgtag tacccattat caggcctaat gattggttgt
    27961 aacaaattgt agtatccagg ctatgaagta ttttattgtt ggtaattgcg ggtttggtat
    28021 gtatatatat ataattgctg gtgtctttgc agcaatatcc aggcccggac aatgcaatac
    28081 gtctaaaata tgtattatgt ttgataatgc cggggttgaa gatggtatcg cttatgacat
    28141 cgaagctttg aggagaagag aagctggcat tgttgctttg ttcccactta taatagtaaa
    28201 ccccatcgcc acctttgggc agactgccaa taaaatcgga aggtttagtc aggctgcaaa
    28261 tggtgtcatt gcctttaatg cggttgtttc tgatagagtc aaggatatgt atggtgatgg
    28321 tattgcttat atcctgacaa gtgtttgatt ttcctgatat tacaagtctg taaagataaa
    28381 aagtgtcggt ttgggtaggt tttggccaga taatgtcatg aacattgttt gtactgtcaa
    28441 aggttgtcca attgatttta tcgaagcttt ttttccaaat gtatttgtat gaattgtctc
    28501 cgccattaac ggcaagtccg cggatggtgt caatatttgc cccaagcgca acccattggg
    28561 tatcagagat aatattgttt ttgatggttg gtaaaacatc aattttaatg gtattgctat
    28621 aactgcggca tacaccggag attaccactc ttctgaattt ggctgaatca ttgagaacag
    28681 gctggtatgt gaatttgttg gatattccgg gtgcaataga atcccaggca gaaatgctgt
    28741 cttgccagag gtatgtatat gttcctggcc ctaaaccacc tttgatagtg tcttctttat
    28801 ctttgatgtt gcggcaaagg aggggcattt gtttattttt acatactaca ttagagtctg
    28861 tatcaagggt tatgaagtta tttttgattt tgggccacac ttttattgtc aagattgtac
    28921 ttgtatcgga gtatgcactc atcactttgt tattttcgtc aagggggtgg acaatacgct
    28981 gataataagt ggttactgtt aagggcccag gttggtatgt catttggttg gcaatagaat
    29041 cccataagat tttgttggta ctttttctcc atacatacct gaatttattg ttggcacctt
    29101 tgggtgtgga tgctttaatc aattttgggg taactccttc gcatatttcc tgatctgcag
    29161 gcatcaggtt gaataagaaa tctttaacag gcataatgag ttttcccatc atttcgccgg
    29221 gagttccgga ggaagcattt ttgaatggca gtgtagggtt gccaaagtta ccttcatctt
    29281 ttcgtaaaat tatgctgtcg ctgaggatcg aattgttaaa tctcaaaaat ccgcctgctc
    29341 caccacctcc cgggcctggc ataggaggta ctgtattgac tgagcctccg tttcctcctt
    29401 taatttcgat gtttagttta tcaagataag tgctgatgtc tataagtatc atgcctccgc
    29461 cgccaccgcc tccggcaccg gctgtagcaa tacctgtaac actttcgcca ttggctttta
    29521 agctgtatcc attggcttta atggcattgg ccataatgat gatgataccg cccccatttc
    29581 ctcctttaga ggcaacataa gggaattgtt gggtactaca tcctcctcct cctccaaata
    29641 ccgcaatagc agggttgggg ttgtttttgt ccgggtaatt gagcaatcca agaccaagtc
    29701 cgagacctcc ttcgcctcca agggttttag gggttgtgtc gcataatggg gcttcgtatc
    29761 caccgttccc gcccatataa aaacaagctc cgcccccacc tccagcgtga tagccattac
    29821 cgcccccccc ggctgtgtat agttttgtac gcccccttgt aaaattggtg tctatcgaaa
    29881 caattgtttc tcctttgaat ccgccaaagg ccatttttga ttttggtaaa tatcccttag
    29941 aaacgtttgt tgaatcgttt tcccagcaag tagcttttga atcgcctgtg gggtttcctc
    30001 ctctaaagcc tttaccagaa acatcaatgt tggcatttag gataagtgta tctgctacaa
    30061 tggccacgat acctcctttg cttccatccc aaggggcaca ggtaacagta ttgttgatgg
    30121 tcttattttt ggtaatgtcg ttaagtgtcc caaccttgac aagttgaagg ttgcaagtgt
    30181 ctgaatagct tacattgaat atggtatctt tagagatact ccgaacaaga attttattgc
    30241 ttattttttt gtgtacaaca tgaaattcaa accttccggt aaaaccgtta ttacctacag
    30301 gagagattga atcgtaactc cagttgactc ctttggcttg atgaaggata ataatatcgc
    30361 tgtctttaat taaagacggg ttggtgacaa agatggtatc cagttggttg gcagctccaa
    30421 aatgtattga gtctattttg taatattgat taatcacttc agttcgtaaa ataattgggt
    30481 tggccaataa aacgagaaga agggcaatta atgtcttttt gaaattgtat ttatgtatat
    30541 gtgtgaaatg cactcttatt ttttttgagg ttcaagtttt tagtttacgt ttattgcaaa
    30601 tttataatag ttattgaaaa gacaatgagt ttgttgtgaa atctttattg taaagaggta
    30661 taacaatccg aaaatgcgcc ccctgattgg aggtgagata ttcgattgag ccattggcgt
    30721 tgtcgattat ttttttgcaa atagccaggc ctaaaccgct tccgctggtt ttagtcgtga
    30781 agtttggcct gaaaagtttg cccaggtttt cgtctgatat gccggggcca ttgtccgaaa
    30841 agtctattac ggccatatta cctgaggctt taagggctat ttcaatagta gcgtcctggg
    30901 tattgtgcat ggcttgtatt gaattggtta taaggttgat aaacaccctg cacaattgtt
    30961 ctttatctgc atacaccata aatgtttttt cgtcttgtaa agaaagaata atgtttacct
    31021 tgttgtgttc aaaaaggttg atggcttgtt tgatggtgct ttcaaggttt attatttcat
    31081 tttttgtctt tggcatttgg gcaaaacttg aaaattcaga ggctatgttt gaaaggtttt
    31141 caatttgttc aaccagggta gctgttacct tgctgaaata catgtcaagg ttagggtgtt
    31201 tgtcgtccca agcccgctga agctgttgaa tacttaattt cattggggtt agaggattct
    31261 ttatttcgtg ggctacttgt ttggccattt cgcgccaagc cgtttcacgt tccgatttgg
    31321 ccaacagttg tgccgagacc tcaagttctt ctactttgtg gttataagct tctattagtt
    31381 cggcaatttc gtcgtgtccg ttatattcta ttttttcgtt ttttgagcca atctttatcc
    31441 tggccatatt ttcctggatg acccttagcg ggcggatgag gttttcggaa aatgcaaaag
    31501 ccaggcccat ggtgataagt accatgatta caaagaagtt gctgattccc tggatgaggg
    31561 agctggtatc atccacctgg ttgtatatca ggtcgtaaaa ctggaggttg atataagctg
    31621 ttagtttgcc ttgtttgttg ataaatgggg tataaatggt attgtatttt tgcttgccaa
    31681 tccattcggc ttgcgtgaat tcatttttgt tcagttgttt tagttgggtt agggcttgca
    31741 tgttcatata tgttccaaca agtccttttt ctattaattc tggccgtgag gtactaataa
    31801 gcttgccttt ggtatcgtaa atattgatgt tggcataaaa aatgtctgaa aggtgtttca
    31861 aatagttttc aaggttactg aattcgtatg atttccagtt aaatctaatg gtttctacat
    31921 tccctaaacg gctttccatt tccagataaa tggtctgcat tttttcccgt atggttttgt
    31981 atgtgttttc gcggttttgt tttataataa aaaagaatgt gatgcaggca atggtaatga
    32041 acgaaagtaa gataatacca gccacagaga ggcgtatttt agtaataaaa ccttttttca
    32101 tatatggcaa aacggatgat gagtcaacgc taaaaagcaa gataaagatg accagcaggc
    32161 taaagatgaa aatgtaagag aatagtacca taaggttata aaatccgata gaagggcggc
    32221 tcaatatgat aatattgtct ttatcgggct tgaaaaacag gtggtcgtaa ccgctgaaag
    32281 aagtttgata atattttcct gaaaatgtgg ggtatttaac caatgttgta ttgtatagaa
    32341 agttaccaga ttggtaagaa agttggtttt tataatattt ggcataagaa tatttctgtg
    32401 ttaccggcga ttggaggttt tcgttggtca gcagtatttg aggatagttt ggagcttcgc
    32461 ttttcaggcg cgaatcaagc tggatatata ggctgacatt ctggtttttg ttgtaatatt
    32521 ttatatgtga gaggtaagtt atgcggccat tactgttttt gagatagaaa aaattagatt
    32581 gggggactgg ttcggctttg ttttttaata attcattgaa gtaaagcgaa caaagttctt
    32641 tttgactgct tgtttgagat attaccgagt cgtttgcctg acaaatggta agctgaaagt
    32701 cgtatttgtc ccaataacct gagaaatatt tttttttggt gtacctgatt atccatgaaa
    32761 catcaacatt ttcgttggtg atacgcaacc tcagggtgct gtcgaggctc agttttttgc
    32821 ccatatcaat caacaggtac tctgctactt tgtcgtgctc tgatgaaagt gaaaatgcta
    32881 tttgtttact ttcgtttatt tgcgattttt gtcggccata aagggcaaaa attattgtat
    32941 atgttgagaa ggcaataacc agccaaaggg tgaggataat ggtgatattt ttatttttga
    33001 tgagcctgat ataaacaata caggcgctga taataagaac aattacaagg tgcagtaccg
    33061 ataactgcct tccgatgata ttaaatatca ggaaagtaaa tacagcgctg ataaaaaact
    33121 ctttgcctgc caatggttct tttttttcaa acaggttacg tccaatgata tcaatgacta
    33181 aagtgagtat gcaaaataca gttgaagcga taagtattga agtgataaaa agttcgggat
    33241 gttcaagtat ttggtttgtt tcgatgttga tattcaggtc tgaaaaaatg ttttctaata
    33301 cgtgacaggc taaaaaataa aggctgaatg tagttactaa caaaaggtat tgaatgcttt
    33361 tgataagcca tttgttatta aaaagtgtac tgtttattga aaaaatattt ttgtgccaca
    33421 gtgatactga aaaatagaca aacatcgaaa taacaaggat gtcgccagat gaaaccgaaa
    33481 agccgagcag gctgatgatt gaattgcaaa aaatctttgt attgtcaaaa aatgagggta
    33541 tctgaagttt tcttaataaa atgaaaagaa acaatgaggc caatgcgtgg ataaaaatat
    33601 atagtatttt acggatactg ttattttgat tttcaagaat agatttcata aacctgaaaa
    33661 ataaaattaa cgaggacaga aggcatactg tgataaaaag ttcgtaaaac aggttatatc
    33721 ttttggtttc gtcaaagttt aatgccagga ctttttgatg aaatttatta aagacgacat
    33781 atttttcatt gacaaaagaa gtagtcattt ttacattttc atcggtattg aaaatgttgt
    33841 taaaagtgtt tttaaggtat ttattttcaa tggggtagtt atttttgagc aaaacaaggc
    33901 ctataatttt gtagtcaaag ttttgtcttt cggctgcgta gcaccatcca ttggaaagtt
    33961 gaagtgccgt acggttgctg ataagatatt ggaggatatc ggtttctaca aacctgttgg
    34021 atgaccaaaa aaccaggctg tcatgtttga aaataaagaa ataagattgg cggctctgtg
    34081 cccagttgcc aagttccttt attttattgg acagaagtat gtcatttgtg gttttgccga
    34141 gctggttgct aatgtcgtca agctgcaagt cgagttccga ttctacttgg agaaaacgtt
    34201 cctggaaatg ttgtagctgg atgtttttgt aaaagtctga ttttaggagg atgtctaagg
    34261 atagtgaaaa cgacagtaaa agaatggcgt acgtcaggaa acgcctcgac attcttttat
    34321 tgtcgttagt ttcatgcata atgcccgggc tattgtttag gcacgtaatc tgtgcgaagc
    34381 accttgaact cggtacgtcg gttgatttgg tttgcaaatt cctggttgtc aggagaaagg
    34441 gtttttataa aggtttcatt aagtatagtt cctaccggta aaaatggaaa ctgtttattg
    34501 agtttttcgt caactacctt cgggacagat tcgccattgc ctttagctga aagcctgtcc
    34561 atagcaatgc cttttgaaat aagataatct acaacacttt gtgcacgacg ttgagatagt
    34621 tctgtgttgg ctttgtcgtt gccaatgtag tctgtgttgg ccatcatttc aatagtaatg
    34681 ttggggttat cgttaagcgt ttctacaagt ttgtccagtg ctgtttttga ttcgggcctt
    34741 aattcggcac gggcaaagtc ataggtaata tcgggtactt caataggttt gtcaataggc
    34801 gtaaggttga tttgtgctgt aaagtcttta ctttttactt caccttttgt gctttgacgg
    34861 gctttattgt tcaggaaccc ttctttaaat gcgatgaaaa cataatctgt atttggcttt
    34921 aacgcaaatt tgaaaatgcc atcggggccg ctcttggtat cagtagtgat accatcactg
    34981 ctgatacatt tgataagggc atcagctaaa ggtttttcac ttctatcatc aatgattttg
    35041 ccagcaatat tgaaacgcag cggaggcaat acaaaagagt ataagtctat atctttcttg
    35101 tcccggccag aactaaagaa acctctttct tcattgggtt ggaaaacaat accataatca
    35161 tcggcatagc tgttaatagg gctacgcata ttttctactg tccatttgcc attttcattt
    35221 ttagctttga aaatatcgag gccacccaaa ccaagatgcc cgtcagatga gaagtataac
    35281 gtaccatcgg cgtgtgaata tggataaagt tcatttcctg gggtattgat ttcggggccc
    35341 aaattttcag ggttgcccca tttgccatct tcattggtag ttactttcca aatgtcgtat
    35401 ccgccaaaac ctcccggcat gtcagaaaca aaatataaag tcttttcatc atttgatatt
    35461 gccggatgga ttacatctac cgtatcgcct gctatatcaa gcatttcagg agtgccaaaa
    35521 gtttcgccgc tgagggtgct taccatgata tgggcactgg cttttttgcc ttttactggc
    35581 ttgcaagaag tataatacat ttttgtaaaa ctcttgttga tactgggcga accttcttca
    35641 aattctgtgt ttatttcatc gccaacagaa accggggcgc tccatttgtc ttttttatcc
    35701 agtttggtat aaaagatgtc aaaggctttt tcgccggtac cttctcttac ctggcctttg
    35761 gcactttcat tagttgaggc aaaaaataca actttgaagt catcgcgtcc aaaggccggg
    35821 ctgttatcac aggcccttga attggcaggt ttaaagtttt ctacaagata gccattgggc
    35881 gagtcaatcc attgctgggc tatacggcat gattgtatgc cattttcgcc gcggatatca
    35941 tccggaacca gctctttata tttacggtac ataggttcgg cttcttttag tttgccttgt
    36001 gcgcgtaatg cattagcgta ataaagatag atgaccgggt tttgaaattc gcggtctatt
    36061 gtttttttgt accacatttc ggcatgttga gggtcgcaca tgtgcctgaa acattcggct
    36121 atctgaaata tgatttcggt ttttttgttt ttgtcttcaa ccacctcgta agcatcgcga
    36181 tattgttcaa tggctttgag ccattcggcg gcagcaaatg aatcattggc ttttttcagt
    36241 ttattgccct gagaaaaagc agatgaggta atgagtatag caaaaattgg taatgcaaag
    36301 tatttcatca tgataaaatt tcattttact taattagtaa tcaaagtttt gttaacgtat
    36361 tttactgttt ggttattttg tgtaaaagta attttttgca tcaaaaaaag cgacaatcct
    36421 atatatttgt ttatcaacat tttgttatgg gggtaaaatt aaacatgtac atttaattcg
    36481 gcaataagtt gtttgaggtt gttaatttgt ttgaccgtgc ccaaaacaaa cagtttatct
    36541 ctgttagaaa ggatggtttc gggcaatggg ttgaccagaa atattttgtt ttgttgtttg
    36601 atgccgataa ttttggcgcc agtatcgtta taactttcga agtcacgcaa agcacggccg
    36661 ttgaggtaat ctgaaatgtt ttgcaacggg atttcgtcta ttagtacatt ggtgtcgcct
    36721 tgttgaatga tgttatctac aaattctact acatcaggtt gtgcaacaat tctggccatg
    36781 cggcgtcctc ctattttgtc gggcatgata acattatctg ccccggcacg gcgtaatttt
    36841 atgtctgagc cttcttctga agctttggct atgatggtta gcttagggtt gagctcgcgg
    36901 gcagtaaggg taaggaatac gttttgtgca tcactgttga tggctgctat cagggctttg
    36961 gcacggcgta tccctgcaag ttcaagagtt tcatcctggg tggcatcccc ttcaatgaaa
    37021 agtacttcgt attggcattc ttgtatttgt tgaatctggg ctgcttcgtt ttctataact
    37081 actacaggga ctttgtgcat ggtaagttca ataagcgctt gcttcccgtt tcggccaaag
    37141 ccgcaaatga taacgtggtt gctaagctta tctatttttt tttgcatctt ttttatttta
    37201 aaatagttac gaaaaacact gtccacaatg tattgtgtta atgccgtaat agtataggca
    37261 aatataccaa aactaatgat tatcaaaaaa gaagtgaagt acattcctat gtccgaaagt
    37321 ggatggacct cgcgaaaccc aacagtagta atggtgataa tggtcataaa cagggcatca
    37381 cggaaattgt agtgttcaat aatgatatag cccaaaattc caaaaatgat aatggacaca
    37441 atgggcaata caagccataa tatccggttt ttcttaggca taggcagtgt ttaaaattta
    37501 gttaaatacc aatggcctat ggtacaatgc aggcattttt gctttgtaca ataatggttg
    37561 tacaattgca gcaatgcctg gctttcaaaa gcattagata ccttggcccc atattttttc
    37621 catgctgcaa ttatactgtt tttttcggca gaaattgctt ctgcaagttg aaaagcctta
    37681 tcttgtaagt gtaaaatttt tctttcacgg ccgtaaacga acaaaaacgg gatgatggta
    37741 ttgattgtta acaggtcaat gctttctttt gaaagctttt taggcgaagc agggcttgtc
    37801 ttgcccaatg tataatgatt ttcccagtat gcagaggtgg aagtgttgaa taatccttct
    37861 atttgtttga tatcctctgc ttctatgagt tttgagaaca atgaagatga gttgttgata
    37921 aggctggcaa gctgggcaag cctgatagtg gggaaatttg ccgggcgcag gcgcatgaat
    37981 ttccatacct gtttttgcag agagataagt tggtattttt ttttcaggaa attgtattct
    38041 gtagctaatt gcgagcagta ctcatcggca ggcttatctg aaagcaggcc tgcctggcca
    38101 agcagcaaag cttcaagttg tataaggttg tttttatgct ttgccagtat ttgtagaggt
    38161 agcgaacgtg ccagcatttc aaaaggttcg gcattgacct tgaagcccag gtttcggcat
    38221 agcatgatat aaaaagcctc ttcccaatgg ttgtttgtaa agttaagtat ctgaagaatt
    38281 tcatccgtcc gtttttcaag acgttctacc atgagtttgg tcaaccattg ctgcaattca
    38341 atccgttcaa tttttggaag gcagctttta caagggatat tggttgaaac cgaaaccagg
    38401 tttttatatt tttcgagcaa tgcgctgtcg tacttcaatt ctataaatgg gattgtttgg
    38461 ccggcactat ttttgatatc ggtatcatgt tcttctacta cgtgcaatat tgtattgtca
    38521 taagctttgt cgagatggtg gttgtgacgt tgccagtctg aggccttttt gtgaacttcg
    38581 ctgcacccgg cccaggttac gccgtttatt ttgatgcggg tattgataaa gtccgggcct
    38641 gaattgaagt tttgttcgcc ggggcttaaa atctccactg tttcgccatt ttgagaggtg
    38701 aagactttgt tctggaagag cctgaacttc cagatatact gcaaaaattc ttcagacatg
    38761 agggttgtgt tttggtttga aaccaaatat acgattttat tgaaaaaatt tgtaaaagaa
    38821 gtttaaattg ttgttgccag attgttctgc aatctgatat attcagccat atagtggcta
    38881 aatcttggta gaaaatttag tattgggaaa ctattaagcc atatagtggc gaaacacata
    38941 tctgtgttat gtaccttggg gaaattggga tgttttattt ctatatttat gtcgcaacta
    39001 tgttgctttt ttgttcagtt tttattctaa aaaggagtag gttgtgtttt ttagaattct
    39061 ttgttttgtc tgtataattc ttgttcaact ttgtcagaga tcctaaaacc taaaattttt
    39121 aattcttcaa ttaaaggtct tattttatca attaccccaa tttgtttagc ttttgttaaa
    39181 actccaagag tgccggttat tttaaacccg attctttgcg cttcttttct gccttttaaa
    39241 tcgtctaaaa caagtaaaca ttgtcatatt caaaagcaag tgaaatagct gaggcttctc
    39301 ctttgtctaa tatggtttca agaattttct gatatttgtt atcttgcggt tctgaaattt
    39361 taatgaattc aggcaaaggg tcaccaaatt cactctcaat ttctggggta atcctgatat
    39421 cttgatatat ttcgtcaagg gatctcaata aatcaagctt agatagagct attaagcaac
    39481 tggtatctgt aatgacgaca gtttcaggca tttttaatat cgcttaataa atcatcagta
    39541 gaactactaa atacagacac accatattta cccataattt ctataaaaga acgtttagat
    39601 aatccgacca ttttagctgc ctgaccagaa gaaataattt catcttcata catttttgat
    39661 gctaaataga tggagaggtc taaagttttt aaatttatat catcagggat atttagtgta
    39721 atagctctca taatcaactt atttttacaa atatacaata tttgcttcag gttttacaat
    39781 tatttgggtg ctacaaatta aactttgtca gggaaatgtt ttttatttcc tggtgacgaa
    39841 tattatttaa atcattgata taaaggtaaa aatataatgt acctttgcgg gctatttaat
    39901 aatttatgat gacatttgaa gaactcgggc tgaaacccga aattttaaaa gctcttcagg
    39961 agcttggatt cgaaaatccc atgccggtgc aggaaaaagt tattcctctt ttactctcaa
    40021 aaaacactga tattgtagca ttggcccaga caggtactgg taaaaccgca gcctttggtt
    40081 tgcccattgt agagcaaacc gatactaagt caaaaaagac tcaagcctta gtgcttgctc
    40141 ctacacgcga actttgtttg cagattgctg atgatttgaa tgatttttcg aaatatgttg
    40201 acagccttag tgtagtgccc gtttatggcg gggcttctat tgaggtacaa ataaaagcat
    40261 tgagaaacgg ggcgcaaata attgttgcta ctccgggacg tcttattgac cttatcaacc
    40321 gtggtgtggc aaaattggag aacgtttcaa aggtagtgct tgacgaggct gatgagatgc
    40381 tcaatatggg ttttgaagac gatttgaaaa ctattttggc tcagatacct gaaaatcgta
    40441 atactttgtt attttcggct actatgcccc ctgctattgc ttctattgct aaaaactaca
    40501 tgagcaatcc gttggaagtt actattggac agcgaaatac cggggctgaa aatgttaagc
    40561 atgtatgcta taccgtacat gccaaagata aataccaggc attgaagcgt attgctgact
    40621 actatcctaa tatttacggc attgtatttt gccgcacacg taaagagact caggaagtag
    40681 ccgacaaact tatgcatgac gggtataatg ccgatgcatt gcacggcgac ctttcgcaat
    40741 cgcagcgcga ctttgtaatg aaccgctttc gccttcggca attgaacttg cttgtcgcta
    40801 cagacgttgc agcaaggggt attgatgtgg atagtttgac gcatgttatc aattataacc
    40861 tccctgatga tgttgaggta tatacacacc gcagtggccg tacaggccgt gcaggtaaaa
    40921 ctggcatttc tattgccatt attaatcttc gcgaaaagca tattatcaaa cagattgaaa
    40981 ggattataaa caagaagttt gagttgcagg atatcccttc aggcagaggt atttgcgaaa
    41041 aacaattatt tcatctcata gataaacttg aacgtgtaga ggttgacgaa gatgaaattg
    41101 ccccattttt gactactacc ttcaaacggc ttgagtggct cgataaagaa gagattatta
    41161 aacgttttat ttcattagag tttaacaggt ttctggagta ttataaaaat atgccagacc
    41221 ttgagatacc tactgaacac tcaaggagag aaggacaacg tgatagcttc cgcgaaggta
    41281 aacgtgaagg ccgtcgtgaa gcaggtgccc ctcgtactgc cgaaaaaggc tatagcaggt
    41341 tattccttaa tcttgggaaa gaagatggtt tgtacccacc cgatttgatt gaattgttaa
    41401 atcagtattc acaaggccgc aggataaaag ttggccgtat tgaactgatg aagaagtttt
    41461 cgttttttga agttcctgag caggatgccc agtttgtggt tgactctatg aaaggggctt
    41521 cagtcagtgg ccgccgcatt gctgttgact gggcttcagg tggagaaaac gaaggcaaac
    41581 agcagagcag gggacgtgat gattttaaaa gatcaggaga aagatctggt tttaagaagt
    41641 tcggagaacg aggaaaccgg ggtgaacatg gtgaccgtaa aagatttgac aaagaccgcg
    41701 gtgaaaaaaa cgaaagaaac aatagtggta atagaagcga caggtccgaa aaaaaagaaa
    41761 aaggtagctt tcgtggcgaa gaaaagaaga aatacggtag aaagaaagag aatagacgat
    41821 agaaattaat tacgaaattt taatcctaaa actaatgtat tcaatttata ttattaccta
    41881 acctgccaat ttaaggcagc atttcccctc ctttacaagg agagcttgtc ctgccccagg
    41941 caggaggcag gggtggttga aacagcttta gtttaaaata cgaaaccgca acaccgcaaa
    42001 gaagaacatt tttctttgcg gtgttctgtg tttagacctt agcaaacttc gcggttgatc
    42061 aaaagcgccc aattcaataa ttccatttta gcctgaaaat gatttacttt taaggtttga
    42121 aaccaactta atttgtttaa aaaatgaaaa agtatttatt ttcggtattg acgcttattt
    42181 gcatgtcaat gcaagggcaa ataaccatgc ctaaggtgtt ttccaaccac atggtattgc
    42241 aacgtgatat taatattcct gtttggggtg gtgcaactcc gggaacaact gttattgctg
    42301 agcttaatgg agttcaggtg atgggtatta cagacgaagc cggtaaatgg atggttcatt
    42361 ttccaaagtt caaagccgga gggccatatt ctttaaaggt aacagagaag ggcaagaaaa
    42421 gttatgcttt tgagttttca gatgtaatga taggtgatgt ttggctggct tccgggcagt
    42481 cgaacatgga atggcaggtg cagcaagcta aagatgctgg aacagaaatc aaaaacgcta
    42541 attaccccaa tatccgtttt ttcagcgtgc ctcatgacat agccttaaaa gtattgtctg
    42601 atacaaaaag tagtagttgg caattgtgcg acaccaataa tgtaaaaaca ttttcggctg
    42661 tggcctatta ttttgccagg gaaattaata aagatgagca tgttgctatt ggaattgttc
    42721 aaagtacttg gggcggaacg ccggttgagg catggacaag ccgcgaaatt ttactttcgt
    42781 cttctattac ccgtaaaagg atactggata atgattcgct gacggagcta catttgaaaa
    42841 atgaagaaac caatttaaaa cgtttttggg aaattgttta taatccgcaa aatgggatgg
    42901 atacaatcgt ccctaaggaa aattatgtgg ataaagactg gagaacaatt tcaatgccca
    42961 gtacattcaa gcaatggggc atgcctcctt acgaaggcat catgtggctg cgaaagacga
    43021 ttacattgcc cgaaagtttt gctggaaaag acctgactat atatcttgga catcccgaaa
    43081 tcaactattc gctttacttc aacgggcaga atatttgtaa aactatatgg aatgcagctt
    43141 taacgcatta ttacaccatt ccgggcaagc tggtaaaggc tggcaataac cttatcagcg
    43201 tgcgtatgtc gttgttgtgg aatgcaggag gctttaaccc tcctgctgtc gagatgttta
    43261 ttactgacgg aaacgaaaag gtaagcattg ccggagattg ggatacaaat attaagcttg
    43321 agcctgctgt accaaaaatt ttcaactttc aatactatcc aactatctta tttaatgcta
    43381 tgcttaaccc tgtaatccct tatgggctaa aaggatttct atggtatcag ggtgaggcca
    43441 atgattcggc acctgcacat tatcgctcgc tgctacctat gatggttgcc gattggcgta
    43501 tcaggtggca gcagggatac ttgccattct tgatagtgca gttgccaaac tatatgaaac
    43561 catgcactga accacaggaa aagagcaact gggctgagat gcgcgaggct cagcaactgg
    43621 caactttgca acccaatgtt ggtatagcct gtaccattga cattggtgaa gaaaacagca
    43681 ttcaccctat aaataagcag gatgttgggc ataggttggc attacaggct aagaaattgg
    43741 tatatgggca tgatgttata gcttcaggcc cggtatttac taatatggaa ataaatggca
    43801 atgaaataac ccttaatttt acatcaactg gtgcaggttt ggtaactaaa aataatgctg
    43861 ctttaaaagg atttgctatt gctggtgaaa ataaaaagtt tgtatgggca gatgcgagga
    43921 ttgttggcaa taccatagtt gtttctgcta aaagcattgc caaacctgtt gctgtgcgtt
    43981 acaactgggc tgataaccct gctggtaact tgtataataa ggaaggtttg cctgcattgc
    44041 ctttcaggac tgattgctgg taatattttt ttttgaaatc acggttgtct ggtaaaagta
    44101 tcaggactaa agtccttcag taagttacta tttgcaaccc cagccataag gctggggtta
    44161 gtcaatgtca tgatgtaatt gggctttagc cctaatattt aattttatat cgggctattt
    44221 ctactttgac agattagcaa taaccttctc ttaaacgcgt agcggtgaaa tatctgtaga
    44281 aaaatgagct ttataatgat taagccacat agtggcgaaa tattatttcg gtgctacgca
    44341 actcatgtga aagtgtattc tctttctaca aagatattgc aactacgttg cttttattga
    44401 acccagcttt tccctcttaa ttctatttta ttagttttca gtttattaaa tctgtcaaag
    44461 tagaactttg gtgttttgaa aattgtaatg gaggttaaaa aaagtaatac aattattgta
    44521 aataattaaa tttcaatgtt ttaaatatgg ttttagcaga tggccttatt caatagtttc
    44581 aataaattgt ttagctgtca ttgcaggtaa ctttgaattt ttaaaatctt taagatttct
    44641 agttataatc aattcttgtt cgttctcaat tgccgagaaa tactgaagtt catcctcaaa
    44701 atcggaaaaa gtattgtcat tcaaagccaa tccaataatt ttatcatcca atggcaaaat
    44761 attcactatc aatcttagtt ttcttaaaat tgattttgca ttgattgaat ccaattgtct
    44821 taataacgca taacttgtat tagcaatcgt taaggatgat actgttaatt caatctgctt
    44881 tttgtcagca agtgaaaata gtatagctgc ttcttcaaaa aaaggttctc ttcgtgataa
    44941 taaatcaatc acaatatttg tatcaacaaa aacttttttc atttatactt ttcttctaaa
    45001 taatcacgat actcctttct gtaatcaaag tcagcaggta aatcaatcac gccgctcaag
    45061 ctctctacaa gaggcgtaat tgaaatcttt ttattctcta ctttctgctt tgttatgcta
    45121 tctagatacg attcaatcat ttttgaaagg ctaatcttat ggctgtgggc ataaatttta
    45181 gctctctcga taacattatc atttaatctc aatgttaatt ttgtttccat aatcttaaaa
    45241 tcaaagttac gtacaaatat aataaattaa tacgtcaatt acaaggtttt ctttttttta
    45301 gttttgttgc cattatttcg ccacgagttg gcttaattat tataaagctc attttaaaca
    45361 aatatcttgc ttttaagcgg ctaagagaag gttttgctaa tctgtcatag taaattaact
    45421 aggtttggcc atatagcttt gccagtttac aatgaccact ttttctacat ccccttcatt
    45481 ttcaagagct ttgaacatgt atccatattc gtaacaaaac cagtattcgt ttttattatt
    45541 tgctacgtac ctgtgagtaa agtggttgaa gttgaaccct tgcataacaa gatattcttt
    45601 tcggactgta gaatggccgg tagggttgag gttacgtagt attgtacggt ttttgcgcag
    45661 gatttgattg atggttttaa ttatcttttc cgattcgttg gtgcgttggt tgttgtatgc
    45721 attgcggcaa tagtagtcac aaaattttct gtcggaacgg cctttttcta agggtttttg
    45781 acaatgaaga cagatttttt tggatatatt ttccatttta ttaaatgttt atagtcgttt
    45841 atattcgatt ttaagcgttt aaaaacgaag ttagtgtttt ttttcatagc aattttgtac
    45901 aaatacattt tttgcaaatg aatccgaaat taccaatagt atttgccaat cggtttgtgc
    45961 atgggagcca taagctgatt aaattttggt ttaaccacaa ccccgatgtg gctgatatgc
    46021 ttgaaaagct cccatgggta aggttttcgt ttcaatacgg gtgtttttat attattgatg
    46081 acggggtgga gattaataaa acggtagaag cattaaaaga taaagtgtta tttaacttaa
    46141 aacgggtaaa tacctttatt tcgaaacctt tgacggtggt agctacgggt tacaatgcct
    46201 tgcatcagcc cgaaactgtc ataaagaagc ctgtggtgaa gtttttgccg gtggtttcgg
    46261 gaggaagaga agtggtgttg ctgacttttg agtacaaccg tgagctttac atgatgttgc
    46321 tgaagtcggg gctggtggtt tgggataaac aactgagggg atttgtgttg ccgtgcgata
    46381 cggattcaat aaaaaatatt gtacggtttt ttagaggaag agtaaaggta ttattcaatt
    46441 cggccatgaa agtgcgtgat accgaattga tgaaactttt gttagagcag gggtgggctt
    46501 cagacgagag ttttgttggc tgtccgttgg cttatttgga taagcttttt ttaaaagagt
    46561 attcgcccaa taccatcagg acatatcatg ctatgtttat ccgttttctc aaccatttca
    46621 aaagatatag catggagcag atagatgcta tggatgctgt gcagataaat gcataccatg
    46681 ctttgttgaa agcaagtaac aagtattctg tatcttatat caaccaatcg atcaatgcag
    46741 ttcgttttta ctatgcagaa gttttgcaaa ggcaggtaga gatggatggg attgacaggc
    46801 cacaacgggg ttttgttttg cccaaggtac ttagcgaggc tgaagtggag gctatttttg
    46861 acaaggtgcg taatataaag catagggcta tgctcatgat gatgtatgct ggtgggttga
    46921 ggattagcga agtactgcaa ctgaaaataa aggatataca aggcgaacgg aaaatgatat
    46981 atatacaagg cggaaagggc aataaggata gatatacggt attgtcggac aagttgttgg
    47041 tattgttgcg ggagtattat aaggtgtata ggcctaaaga atatttgttt gaggggcagt
    47101 atggaggggc atattcggca gaaagcatta ggagtgtgtt aaagcgggct atagaagagt
    47161 caaatttgaa caagcgagta acaccacaca tgttgcggca tagttttgcc acacatttat
    47221 tggagcgagg caccgatttg aggtatatcc aggagatgct ggggcacgaa tcgaccaaga
    47281 cgactgagat atatacccat gtatcaaaaa aacagatatc gaagattata agtccggctg
    47341 attttttgaa aatatgaaat taagtatata catttgaata aaatctgata gcagtgatag
    47401 gataataaaa ctttaaacaa tgataaaata taaaaaacta accagcaatg aattgtaagc
    47461 atgatgataa ggacaatata aaggttattc actgtgctat tagttagttg tgcactatgc
    47521 gcctgctacc agcaatcggg cgattaaata cataaaaagt ctgcataaga ctatttctaa
    47581 gcattttgac tgtaaacttg tacgcggatt agatttgata aacgattaga ttactttgaa
    47641 atagtctcgt gccgacttta ttgggataga atagatagat aaactggata acagacgaat
    47701 aatgcctttt taactgacaa cgacaggatt tgattaaatg acaagtgcta catagatttg
    47761 ctctttgatt aacttgattt tattggatta catggattaa gtataacgat tagattataa
    47821 acactattac tttgaattga tttgcttttg gataacagat tattggatta attcatagat
    47881 aacagataaa tcaatatttt caacgattgc tgatttcaat ctcaatgatt tcagggtaat
    47941 tttcggatta attcatagat aatagataaa taagcacatt caacgattac taatttcaaa
    48001 tccaataact acaagataaa tctcggatta attcttagat aacaggatat tcgtgctgtt
    48061 tagattgaat aacgataaac aataatagat ttgtggtttc tggcgatgct tgttccgatt
    48121 aatagatttg ataagacaaa tacaacgata accgtcaaag acttatgatt actgagaaat
    48181 ataaagcctg aagcccagta aaacagaata aaatacagtg cccaacaatg cctatagcaa
    48241 aggcgggcta cacaaatttg caaatgtcca catcaacgtt tatatttgtt caaggtttat
    48301 ggtcgggggc tcggaaaccc gcccttgcca taggccaggc gttggttgca atgttaagac
    48361 aaaaattttt ggttcaaaaa tctaatacaa ctttttctaa aaaaattaat attttcgtaa
    48421 aaacataaaa caaaaaatta tgagt
//
LOCUS       DAOSPF010000022        48436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_790_length_48436_cov_6.919349, whole genome shotgun sequence.
ACCESSION   DAOSPF010000022 DAOSPF010000000
VERSION     DAOSPF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_48436_cov_6.919349"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..506
                     /locus_tag="PK252_06170"
     CDS             129..506
                     /locus_tag="PK252_06170"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14329.1"
                     /translation="MENLEIQGTNDTPYIFCDAEKKEITLTGRSLPENPFKIYEEVFK
                     WGEQYDGESIQINFKLEYFNTASSKQIFTLIKTFVENKTVKEIKVKWHYEEGDYDSKE
                     TGEHFSHLFRIPFDFIEYAESGF"
     gene            658..2382
                     /locus_tag="PK252_06175"
     CDS             658..2382
                     /locus_tag="PK252_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14330.1"
                     /translation="MRKVHYSKLAVLLSLAVVLGSCSGLKKMQEQASTTKFELTPKVL
                     EMHNGEVNATISGKFPPKYFNKKAVVTVTPIIKYEGGQIELQSYKLQGEDVQANDKVI
                     PFEAGGDFSYSDKFAYKDEMMLSKVSVQVSAQMKKENLVIGTFEIGDGIIVTPKLVRL
                     EPKASELTQNQFVRNVAQDKLANIKYLINQAEIRNTELKKDEVKQLNDFVKTIAKDPK
                     FNIKGIELSSYASPDGPENKNAELAGKRESAAVKYFKSELKKAKLDKILKDSLFSMLK
                     TPEDWDGFKTSLEKSDVKDKDLILRVLSMYSDPAVREKEIRNISQAFDELKVKILPEL
                     RRSKLIVKAEAIGRSDEELISLADSNPDALSLEEVVYTANMIQDLDKKAALYAKASEK
                     YPNDVRLKNNLGYVYFKQGKLDEAKAALEQAKSIENNDAVKNNLGAIALIKGDVAAAE
                     ELFTAALGAGAAPNYNLGIVNIIKGKYDIAVSYFGNECDANAALAKLLNKNYEAAQNA
                     MGCSKDESAQGYYLRAVIGARMDNSDLVFNNLRAAVGKDIQLKNYAKKDVEFLKYFND
                     ETFKSIIQ"
     gene            complement(2455..3297)
                     /gene="folP"
                     /locus_tag="PK252_06180"
     CDS             complement(2455..3297)
                     /gene="folP"
                     /locus_tag="PK252_06180"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT14331.1"
                     /translation="MISNKYTINVNGSLVEIDHPLVMGIVNITPDSFFSGSRFVTEKE
                     IIDRTQQIIDEGGSIVDIGAYSTRPGAEYVDEATEKARLKVALTVIRANFPEVIISLD
                     TFRAEIARWTVEKYNIGIVNDISGGTLDEKMFQTVASLKVPYILMHMRGNPSNMNDYA
                     VYNDVVKEVIFELSEKVDKLKEMGVSDIIIDPGFGFAKTLEQNYQLLNKLEVFQFFDL
                     PVLVGISRKSMIYRLLQTNPEQAINGTTALNTIALLKGAKILRVHDVKEAVETVKIVS
                     QLSR"
     gene            complement(3304..4452)
                     /locus_tag="PK252_06185"
     CDS             complement(3304..4452)
                     /locus_tag="PK252_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT14332.1"
                     /translation="MKSIKSILQKWLVQFNEFTYKIGFKQIDRYILKKFLGTFVYSII
                     LVMSVAIVFDVAEKIDDFVETKTPMQAIIFDYYLNFIPYFTILFSHLFTFISVVFFTS
                     KLAYRTEIIAILSSGVSYKRMLVPYMWGATFIAIMSYLLMNFVIPNANKVRLEFEDRY
                     IHNSPFQYNQRNIHKQVRPGMVIYMESYSNSANTGYKVSIENFENGTLKSKLMGDFMT
                     WDSTKSKWTIRNYYIRTIDSLNEKITKGKAIDTTLYIYPGDFKRRVNVVESMDLFELN
                     AFIEEQELQGGENIEAYQIERYKRVSLPFSTYILTIIGVCVSSRKTRGGTGVHIGIGM
                     GLCFSYIVFMQFSQQFAISGSLSPLLAVWLPNIIYAIIAYWFYRKVPK"
     gene            complement(4473..5807)
                     /gene="ffh"
                     /locus_tag="PK252_06190"
     CDS             complement(4473..5807)
                     /gene="ffh"
                     /locus_tag="PK252_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT14333.1"
                     /translation="MFENLSEKLEKSFKRIRGEGRITEINVAETLKEVRKALLDADVS
                     YKIAKNFTDTVKQKALGQEVLTSLKPGQLLVKIVHDELVELMGGAYQDINLKGSPSII
                     LIAGLQGSGKTTFSGKLANYLRTKKGKSVLLVAGDVYRPAAIEQLKVLGNQVGVQVYT
                     EENNTNPVNIAQSAIKHAKQHGHNVVIIDTAGRLSVDEAMMNEIAAIKKAVDPNEILF
                     VVDSMTGQDAVNTARAFNDRLNFDGVVLTKLDGDTRGGAALTVKSEVNKPIKFVSTGE
                     KMEAIDIFYPDRMADRILGMGDIVTLVEKAQEQFNAEEARKLQKKIAKNQFDFDDFLG
                     QIQQIKKMGNIKDLASMIPGVGKALKNMDIDDNAFKGVEAIIKSMTPKERQNPDLING
                     SRRKRIADGSGTNIQEVNRLLKQFEDTRKMMKMMSGGNAAKFANSLKNMRPR"
     gene            complement(6372..6809)
                     /gene="tnpA"
                     /locus_tag="PK252_06195"
     CDS             complement(6372..6809)
                     /gene="tnpA"
                     /locus_tag="PK252_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOT14334.1"
                     /translation="MTEFSIIKKVWLHIVLQTNNRLPLILPEMEKDVYATLRECCKNV
                     SLQVSEIGGTPDHIHLLVLDEDKSSVSFNVQSFALESAAVINEKHFKAGSFAWHSQVY
                     VYSVSHSQAQKVGEYIANQKEIHKTRNSKKEIEEFAALHQLNG"
     gene            complement(6802..7296)
                     /locus_tag="PK252_06200"
     CDS             complement(6802..7296)
                     /locus_tag="PK252_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOT14335.1"
                     /translation="MNGPAELYQLLDQLQVSFDYYEHPAAPTIEEAKKYWADIEATHC
                     KNLFFRNHKGDRHYLVVLHYQYKLNIHDLEKRLKQGKLTFASEQRMWKYLQVKPGSVS
                     PLALFADTEKHVTVFFDEKLKEASKLSFHPCINTASLVIPMPHFMRFIEFCGNTHYFV
                     SMYD"
     gene            complement(7301..8332)
                     /locus_tag="PK252_06205"
     CDS             complement(7301..8332)
                     /locus_tag="PK252_06205"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOT14336.1"
                     /translation="MGFRIGDKVKFLNEAGGGVIKKFLANNQAMVEIEDGFEINTLLS
                     NLVHDTSAQHIALDVEPEAKPTKVEKPKNFVKEPQDKASGPTFSEKKIVSDDKALVCL
                     AIVPVGLDSAFAYSLFDLYILNDSNYDILFTAAQVKDNIYRLIEKGELEAGMSMNIGQ
                     YTLEKLKTIQRIVVDVLYFSTSSYISKVPTTHNSDFAGIAWNNPETFSKNDYFDEPAI
                     IIQLGRKTSFKSSSKTNKDVVKIESEAQVVNETEEVDLHIEEITDSIEGLQPGEILQM
                     QMARFETTLEGALKAKTKRIVYIHGVGNGKLRYEIQKVLKNKYPSLQYQDASYAEYGF
                     GATMVILKK"
     gene            8658..10238
                     /locus_tag="PK252_06210"
     CDS             8658..10238
                     /locus_tag="PK252_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOT14337.1"
                     /translation="MSLQDEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIKKTAMSDFMEIEKQRGISVSTSVMGFEYKGKKINILDTPGHEDFAEDTYRTLTA
                     VDSVIIVIDSAKGVETQTRRLMDVCRMRSTPVMIFVNKLDRPGKDPFELLDEIQEELK
                     IHVRPLSWPISNGSTFKGVYNIYEKNLNLFEGADKTTIEEAIEFKDIENPELENYIGE
                     KYAKTLREELELVEGVYEPFDHELYSEGHLAPVFFGSALNNFGVRELLDCFIEIAPSP
                     KGFTTVERSVDPYEQKFTGFVFKIHANMDPNHRDRIAFVRICSGTFMRNTNYLHVRQG
                     KQMKFSSPTAFMADKKSIIDEAFPGDVIGLHDTGNFKIGDTLTEGEILHFKGIPSFSP
                     ELFKYVENADPLKSKQLAKGLEQLMDEGVAQLFINKSNGRKIIGTVGALQFEVLQHRL
                     LHEYGASCRYEPINLYKACWMVSSNNAQLEDFKKRKYQYIAEDKYGRDVFLADSSYAL
                     SMAQEKFPDIKFHFTSEF"
     gene            10465..10836
                     /locus_tag="PK252_06215"
     CDS             10465..10836
                     /locus_tag="PK252_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT14338.1"
                     /translation="MKKIEAIIRKSKFDDVKIALQTIDIQFFTYWDVTGVGKETEGHI
                     YRGAVYQANQIQRTMLSIVVRDHNAQKTVDAIIAAARTGQFGDGKIFVSDIEEVIRIR
                     TGEKGPEALYLKEEEKPLEIK"
     gene            complement(10957..11394)
                     /locus_tag="PK252_06220"
     CDS             complement(10957..11394)
                     /locus_tag="PK252_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT14339.1"
                     /translation="MSVNKVILVGNVGKDPEVRHLENGTAVSTIALATSEYYTNKSGE
                     KVSTTEWHNVVLWRSLADLAEKYIRKGSQIYVEGKIRSRSYDDKDGNKRYITEIVADT
                     VQLLGRKPEDGNNSGAQTSAGTVAKPQEEPFQVSESPIDDLPF"
     gene            complement(11447..12517)
                     /gene="mutY"
                     /locus_tag="PK252_06225"
     CDS             complement(11447..12517)
                     /gene="mutY"
                     /locus_tag="PK252_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT14340.1"
                     /translation="MHEFTFFKKSIISWFAQNKRDLPWRDTNNAYYIWISEIILQQTR
                     VNQGINYYYRFIDQFPDVVSLANANIDEVMKCWQGLGYYSRARNLHAGAMQIVNNFGG
                     KMPNSYAELIKIKGIGRYTAAAIASIAFAERVPAIDGNVFRVLSRLFADSTCIDDDNG
                     YTHFYKIAQSMMVDCSPSDFNQALMEFGALVCLPRNPDCAGCPIMSVCMAFQTNQMLS
                     FPVKKQKKAPTSRFFNYFIFQYKQSFFLRKRDNEDIWKGLYEFPMSETKELLTVEKKY
                     FEKIINEYVGDIQWNIKKHSDAVKHQLTHRTVVATFYHIELSALPNELSEYICIPKSQ
                     FHQYPVPKVIERYMEKEFDFKD"
     gene            12655..12939
                     /locus_tag="PK252_06230"
     CDS             12655..12939
                     /locus_tag="PK252_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT14341.1"
                     /translation="MTKAEIVNEISKVTGIEKVTVQKTVEAFMESIKDSMVKDKNIYL
                     RGFGSFIVKKRAQKTARNISKNTTIVIPAHNIPAFKPAKSFVNKVKSSIK"
     gene            13203..14747
                     /locus_tag="PK252_06235"
     CDS             13203..14747
                     /locus_tag="PK252_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT14342.1"
                     /translation="MDTNNELIIHVTESDIDIAILENRQLVELHKEKNNNQFSVGDIY
                     LGRIKKIMPGLNAAFVDVGSEKDAFLHYHDLGPQVLSLNKYLKQSLSKKGKPISLSKF
                     ENELDINKNGKIADVLQQGQMVLVQIVKEPISTKGPRLSSELSLAGRNLVLMPFSDKI
                     SVSQKIESAEERNRLRTLIQSIKPKNFGVIIRTVAQDKKVATFDLELRSLVKKWESAF
                     ENIKGTTTPKRVISEVSRTSAILRDLINASFSNIYVDDEATCQEIKEYLQEIAPEKAD
                     IVKFYNESTPIFEHFNIEKAIKLLFGRTVSVRSGAYLVIEHTEALHVIDVNSGNRSRA
                     AKDQETNALEVNLAACDEIARQLRLRDMGGIIVVDFIDMQSNDHKQQVYDKMKNLLAE
                     QKAKHNVLPLSKFGLMQITRQRVRPETNIDTNEKCPTCKGTGEISSSVLFENELDIAL
                     SKYFEKSKNKKITLRVHPYIAAYLKKGFPSIRMVWSLRYKCFIKVEAAFALTFFQYKI
                     DPPAES"
     gene            15067..18168
                     /locus_tag="PK252_06240"
     CDS             15067..18168
                     /locus_tag="PK252_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT14343.1"
                     /translation="MIKKIITLIFTLFSAFIIANGQNNITITGNVTSAEDNLGVIGAA
                     VYVKGTTIGTTTDLDGNYKLTVPEGTKTLVFSYVGLASQEIAIEGLTQINVVLKPDVF
                     KMDEVVVAGVASATPKRKLSVSVAKVGADDLQNVPASSASTALQGKVAGITVVGSGSP
                     GQGASIRLRSSTSIYGSQSPLIIVDGVMIDGDLADINADDIETIEVVKGAAASALYGS
                     RAGSGVISIKTKRGSSAAEGTTEIKFRNEFGFSQLARKIKLAEHHPYLVGADYNQGTY
                     TKYDSVTYPVGYVQGYDNRIKGSRMLDYDHYADNPYAFVKDPQDEMFQNGLFYTNYLS
                     IASNSNKTGILISFENNKNTGIVFHKKGQSRQNVRINVDHKLNDIFKISASSLFTQNE
                     IDMSTTDAYTLGAFSDVLFMNPDVNLNMDAPASDTTILKKYYIKPDNWAITGNPKHTL
                     YYEEVTEKRNGMLLNLNGSANLLSWLVFDAEYSLEKRFTYYSRFVPIGFLGSNKENEK
                     NGSITKSTLPQTSQSVQATLNFNKKFNELVLKGKASYLFEKQQQEYFYGAGDSLLANG
                     VKSLSALGKGIIVGSDVIDIRARDYFGILDLDYKDRYIASILYRYDGSSLFGDENKWN
                     PYYRVSLAYRLSEDFKIPNVQELKLRYSIGTSGQRPGYSYQYETYNIENGQYVPYSAA
                     NKAIKPSETKETELGFNMQFFERFEADFTYSFNKTTNDFLPVPQGAASGFKIRWQNAA
                     TIEGNSMEFTLSTQAIKTKDFEWRLSLSYDKVRQKIADLYGSTPNFFTGPNSSFQYFY
                     NREGENYGVMYGYEWVKSLEQMKNQLKADDNIDNYTVNSDGYVIKKGTEGTVYEKPIA
                     LKDASGSEAFVKIADMNADFNMSLNSTFSYKKITLSMLWSWKQGGDIYNYTKQNLFLD
                     KRAGDYDQASKPDYQKKAIDYYNTFYDGFKANSYFVEDGSYIKLRELSLYYNVGNIKI
                     SGKTIFKAMKIGLLGRNLLTFTKYSGWDPEVASGTDRTNFIVDMFNYPNFRTYTASIE
                     LNF"
     gene            18189..19718
                     /locus_tag="PK252_06245"
     CDS             18189..19718
                     /locus_tag="PK252_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14344.1"
                     /translation="MTIYCKNTISKAFQVIALALTLTACESLDVENTDAPDRKDLLNS
                     SRDFGGFAQNIYATYWQAIHNEAFSSPQIASLAAADQITASWYTFGCYELSQEPRIEW
                     NNTPNYEYKEITYTFFTRMYTVLFMSNTTINFLLQGKTISSNNKTNSGYLSYAYMMQG
                     IALGQLALTFDSSQIVKETTKIENLAFSPYNEVMDSALESLEKAISIADTTTFNLGSN
                     IVNGVTLDNKLLSRVCHSFIARFLTLNARNKSEYAATDWPRVLANAKKGINEDFGPQG
                     DGNNKWIDEIARFITNVDNSQLFFCKTDNRIINLLDPSYPSRYWKNGHAKVVHPGYQS
                     GEAISKDYRLTSDFEYSPTIRFYADRGTVHYSHYRFKRFDNIRKTGLGKLIEYPEYEN
                     KLYAIEAMANMGQTENAINAINNIAYPRRTRGKLSPVDITTSKKELLEIIFYERDIEL
                     FGQGYLIAFCDMRRRDKLQKGTPLHFPVPANELQTHKRNIYTYGGVENADGTNTSNGG
                     W"
     gene            19833..20582
                     /locus_tag="PK252_06250"
     CDS             19833..20582
                     /locus_tag="PK252_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14345.1"
                     /translation="MNIKTYINIVSIGIIASLVSSCGNDDYDWNNVIPGSQRITTLDN
                     DTVKVKIDTIEGNNTDVVTLKAIAHGGSTFDWIASSNVLIPQNDAEKNYIVHVKANST
                     QDTYAWLKVQETTYGGIKGKPDSMQFFIIGYCEFNKQQLLGNGSFVSKMSYYAPFDVN
                     LSEGKGDTILYNNFNNMRWQLKLVISKDVYQKITIPANQYFVYNDEIVTVKGNGKYNT
                     CKSLFVINYAICRLSGDTLAYSSGVDSLRIK"
     gene            complement(20586..22592)
                     /locus_tag="PK252_06255"
     CDS             complement(20586..22592)
                     /locus_tag="PK252_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14346.1"
                     /translation="MKLVKILLTLLFVTVSFFKAPIIAANSDTTGLYAFGVKVSDLAD
                     ADSALFFSELAKYREIGEKLNADSLKGKSFLMQGEYYKKRGNTQAAIESFNKALDFFN
                     SSRKTTLIAKAYNNLGVTMLDVGKYEPALKNLFNALRRFEQAKDVYNEARVVLNIGLV
                     FYYQKNYDLALFYYNKSLKLRESIKDIKGMALAYNNIGIVYYFKGRTGAAMEYFKKAL
                     AIYEKNNMIRAMSMPLFNIGEIHYETGQYDSALVYYQKSYRIDSMLDDKASLTKSLTK
                     MAGIYNKHSRREEAARYANQALELAKLVDSKEDIRDCYLVLSDIYSDKGLYKEAWKCS
                     EKASLYKDSLYNSQSAEKIAEMQTLYETEKKDLMLKSQHDKIEHQKLITRILAAGLCI
                     VLALLILVFIEYYQKKKAYIILNIQKKQITDSINYASRIQAAILPHEEVLNTYLPEYF
                     IYFSPKDIVSGDFYWVSVHDGRTVIAVADCTGHGVPGAFMSMLGFAYLNEIVSKNKDI
                     EAKDILNQLRIKVKTLLNQDDKTGAKDGMDIALVIINRNKTKIQYSGANNPLLVVRDG
                     KINSLLPDKMPIGIYPTEKESFSQQEFELQEGDMMYMFSDGYADQFGGERSKKLGREG
                     FYQLLLEASVMPVKAQRYFLEEKFLQWSLNLEQMDDVLVLGMKV"
     gene            complement(22698..23507)
                     /gene="larE"
                     /locus_tag="PK252_06260"
     CDS             complement(22698..23507)
                     /gene="larE"
                     /locus_tag="PK252_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOT14347.1"
                     /translation="MDEKFKSLTAYIKKLEKVAIAFSGGVDSTFLLAVARQAVGDDAI
                     GITIDSPALPRYELEDARHLAQIIGVKLIIVESNEIEDEVRQNTVDRCYFCKKIEFGN
                     VIEEAAKNGIKYVLDGSNADDVNDFRPGAKAIDELQVHSPLKTHGFTKDEIRKYSKMM
                     GLPTWDKPAYACLFSRIPYGTAIKEEDLVKIEKSEKYLIDKGFRTVRVRCHDKLARIE
                     VAADQRVQIMSEPLASEVSAALRSFGFDFVTVDMLGYRMGSFNETLGADLK"
     gene            23994..25007
                     /locus_tag="PK252_06265"
     CDS             23994..25007
                     /locus_tag="PK252_06265"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOT14348.1"
                     /translation="MKNLKATYMGLELKNPIIAGSSNLSFDIDSLKGLEDAGASAIVF
                     KSLFEEQILLEKAILEDDLDEYAERHAEMTSLFPKLEHAGAKEYLLKLEKARKVVSVP
                     LFASLNAIFKETWVEYARQIEQTGVDGIELNFFAIPKDADITGSDIEKQQLAIVHEIK
                     KNINIPISVKLSPFYSNTLNFIKSLDKEGVDGLVLFNRFYNPDIDIENMKFDSLHHLS
                     HNDESKLPLRYAGLLYSNIAASICCNSGIHQGTDVIKMLLAGADCVQVVSTLYRNKLT
                     HIKTMLHDIELWMDFQNIKSLEEIRGKLSRKNLHDPFVYQRAQYIDLLIKSDELMNKY
                     TLR"
     gene            25187..28462
                     /locus_tag="PK252_06270"
     CDS             25187..28462
                     /locus_tag="PK252_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOT14349.1"
                     /translation="MNKLINIQAFRILFVLLLIILIISCKINNNPQNYKEIFQKSETY
                     FSNNEFFNASRGYQSYINCPQIDEYSLFISYGKLFICLHRMKDKNARQAYDSALAHIP
                     KLKDKAVEDLLYKIKSIDLQKNEEYLKAIDLTRKEICKYKLDDTLSIEHFHNLGGLYQ
                     SIGNKDSALYYYIKAKQLLDKKKDKFNQLSADIYNTYAYCQRYYLNTPLNAAKYYDSL
                     LYVSEKILHKDSDYYAWNLFNCGIFYQVIGANNKALHNYYKAFETYQKINGNFDEECN
                     YIQVFIASIELHLGNIQKASDKINNSDLYYKSTKDYSNIAKAYIFDAYLKTKLGKYRE
                     ALNIYDKYIRLADSLKLSTKETHYLDIATCYVKINQKDSANYWFEQCFNTLKSPKKNY
                     LIMLHTFLWEYVGFCLANDHIAKASEIIRFYESNLLKSYGNNSPNYALMLLCKARIAD
                     YQKEYAKGTDFYLQALKILENKSYANSQTNKNISLQRYNNLLAKEIIDCTTGLARDFK
                     HLAQKSTDKIKFLQKSFEYYKQTLELMGQMNGNIPVEWDKLRFFEQFNAVKKEILEVK
                     IAQYRNEPQVGKKNQLLLEAFEIADANKLSIMVEKLKEKQFKQQLGIHDSILKKDEKL
                     SENISFLQHIIDEEEKQEHPDNQMLEKYYKLIFKTYNEYDSLREFINKLYPAIQDIYG
                     ANHLRTSYKQMIHCLPQNELLIEYAFTADSLYAFAVKNGSISLFSVPSEIITSNIQDF
                     RALLTNVTDQAYTHQGITTYTNTAYSIYKQAVFPAIQNASFERLTIIPDGMLTFIPFE
                     ALVTHSATRQNADYGTLPYLCLSANINYTYSLALYSGQLSHYSNVASGITGYAPSYDK
                     SSPLSPLRNAELRKKLKNLKGASDEVYSLVNAYGGRKLIGKTATETAFKQAHNSKHIL
                     HLAMHTVIDDENPMYSKLAFAEEKDSVNEGFLNTYEIYPLKINSPLVVLSACNTGYGK
                     IIDGEGIVNLTRGFYYAGCNSLLITLWNISDQSSVQLMKLFYNNLDAHDDKNAALAKA
                     KVSYIANSDSRYSHPYYWAGFIHSGNAAPLAISKIETSAYIILSVLLLITIGLFNTKW
                     KFK"
     gene            28498..29310
                     /locus_tag="PK252_06275"
     CDS             28498..29310
                     /locus_tag="PK252_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOT14350.1"
                     /translation="MLKKVFISAFIFAVSGIGIHAQCNVAESVFQPEEKYDYLVSYHL
                     SSIWVKAGVVSFVTHKTTYRGKDSWFLESYGSSLPRFDWFFRVRDTFRAYTDADTFVP
                     DFAERVSDEGGYIVIEQYNYDKIRSKIFSRVYSSGKPWKYDTFKYEPCINDVLTAVYA
                     ARNQDFSNIKPQEKVPFQTLVFGKIQPLYIRFLGIETIQLKNGQKFNCLKFSSKLVEG
                     TIFKGGEDMFVWVTNDKNHIAVKIQAKIVIGSVVAELTSFKNNKYPLTSLQK"
     gene            29371..30573
                     /locus_tag="PK252_06280"
     CDS             29371..30573
                     /locus_tag="PK252_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOT14351.1"
                     /translation="MQKQIPIAEYTYDLPDERIAKYPLSHRDLSKLLVYDKGNILSEK
                     FGSISQYLPENSLLVFNNTKVIKARMMFQKATGAAIEIFCLEPENPSDYQQAFNQTRR
                     CSWKCLVGNLKKWKSGEITAIVNINNKPVTIKALLLQNMQQWQIVQFNWDAENITFGQ
                     LLENAGQIPIPPYLNRESENIDLLRYQTIYSQHEGSVAAPTAGLHFTDDVFKALSSKG
                     VEQCAVTLHVGAGTFMPVKTDDALQHDMHAERFYIPLQNIEKLLKNIGNITAVGTTSM
                     RALESLYQIGVALILKKNNPFLVSQWQYRQVNEQITALQALEAIINYCKEKNLKGIEA
                     ETRIMIVPGYKFRMVNRLITNFHQPQSTLLLLVAAFIGPDWRKIYDYALANNYRFLSY
                     GDSSLLIP"
     gene            complement(30647..31822)
                     /gene="mnmA"
                     /locus_tag="PK252_06285"
     CDS             complement(30647..31822)
                     /gene="mnmA"
                     /locus_tag="PK252_06285"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT14352.1"
                     /translation="MAQKVVIGLSGGVDSSVAAWLLKQDGYEVIGLFMINWHDTTGTL
                     YGDCAWKDDLTFAELVARKLDIPLHTVDLSVFYKQRVVDYMFSEYEKGRTPNPDVLCN
                     REIKFDLFVDAAKKYGAEYVATGHYARCEAIETENGQKYRLLAGVDGNKDQSYFLCQL
                     SQKQLSTAMFPIGHITKPEVRQIAESLGLATAQRKDSQGVCFVGKIDLPVFLQQKLKA
                     VEGVVVEIPSDCPVYKEKNNLDLYDTMQPYSYTIDMGKVVGKHSGAHFYTIGQRKGLN
                     IGGMQKPLFVIAIDVVNNIVYVGQGEDHPGLYRKALYVSEHDIHWIRPDLALDEGDEQ
                     RFRVRIRYRQPLQDASVLKLENGLAIVFDQAQRGVTPGQFAAWYAEDELVGSGVIAY"
     gene            complement(31825..32397)
                     /locus_tag="PK252_06290"
     CDS             complement(31825..32397)
                     /locus_tag="PK252_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HOT14353.1"
                     /translation="MEGEQRRLRKLNDEQWKEIPGTEGKYLISNYGRIKSFHFKKEGQ
                     IVKFYKMKNYLAVSLQKVTYSKYVHKLVAWVWLEKPSDKHIHVMHIDGNIRNNYYKNL
                     QWITREESLKRVSELMKEKTQKKSKEELITYSKLNEKDVKLIKSMLLRKTPQHIIAKM
                     FKVSEMQITRIKRGENWAHIQPDPIAPENE"
     gene            complement(32442..32606)
                     /locus_tag="PK252_06295"
     CDS             complement(32442..32606)
                     /locus_tag="PK252_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOT14354.1"
                     /translation="MKKYRCIICGYVYDPQEGDPDSGIAPGTSFEDIPETWSCPVCGV
                     SKKDFVEVDF"
     gene            complement(32699..33820)
                     /gene="pdxB"
                     /locus_tag="PK252_06300"
     CDS             complement(32699..33820)
                     /gene="pdxB"
                     /locus_tag="PK252_06300"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HOT14355.1"
                     /translation="MRVIADQKIPFLKGILEPYCNIEYLAAKDITKELVKDADALIIR
                     TRTLCNADLLNGSKVKFIVTATIGFDHIDTEYCEKNGIVWTNCPGCNAGSVMQYMAAA
                     FAHLATEKGFYFVEKSLGVVGVGHVGTRVVRLAETLGMRVFLNDPPLVKAKNLCGYIS
                     FDGILRESDIISFHTPLTYSGEFKTFHLLDEKKMQKVNKGTIIINTSRGEVVDNLALL
                     NSLQNGRIDTAVVDVWENEPALNVGLLDKSAIGTSHIAGYSADGKANATFMSVKALNN
                     FFRLGIDKIKIDIPQPQQPLIEIDCSNMGDQQVICNAILHTYPISSDSNMLKSHPQDF
                     ESYRENYAVRREFNAYTVKLIHGKTIQKDILKKLGFNVI"
     gene            complement(34255..35502)
                     /gene="fabF"
                     /locus_tag="PK252_06305"
     CDS             complement(34255..35502)
                     /gene="fabF"
                     /locus_tag="PK252_06305"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT14356.1"
                     /translation="MKRVVVTGLGAVTPLGNNVSSFWDALVQGKSGIANITRFDASAF
                     KTRIAGEVKGFDPLMFIEKTEVRKTDLFSQYAIASVEECIKDAGLDVTKINQQKAGII
                     WGTGIGGIETFENEVVSFSKNPEMPRFSPFFITKMISNMAPGLISIRYGLKGISYVTT
                     SACASSNSAIIDAVNFIKLGKADVIFTGGSEACITKAAVGGFSSMKALSERNDSPQTA
                     SRPFDVSRDGFVMGEGSGVLLLEELEHAKKRGAKIYCEITGSGFSSDAYHIAAPHPDG
                     EGAVAAMLDAINEAGLTPADVDYVSTHGTSTPVGDLSECHALCKVFEKSLDKLHVSAT
                     KSMTGHLLGAAGAIEAIACVKAIETGIVPPTINVNEIDPAIDKRLNVTPNKAVKKEVN
                     VAINNTFGFGGHVFVSLFRKYKG"
     gene            complement(35588..36586)
                     /locus_tag="PK252_06310"
     CDS             complement(35588..36586)
                     /locus_tag="PK252_06310"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOT14357.1"
                     /translation="MNKIKAAITGIEAYLPEYVLTNEELSKMVDTSDEWIMQRVGIKE
                     RRILKDKDKATAFMGGEAAKKLLAKTNTSPDTIDMVIVATVTPDMAFPSTACLVADYA
                     GIKNAWGYDVSAACSSFLFTLQTASSFIESGKYKKILLVTADKMSSITDYTDRTTCVL
                     FGDAAVAMLIEPTTQEVGVMDAMLHIDGSGKDHLNLKAGGSLRPASHETVDAKMHYIY
                     QDGKAVFKVAVSKMADVSVAMMEKHNLRPDDIAWLIPHQANLRIIDAVGNRMGLPKEK
                     VMVNIERYGNTTAATIPLCLWELERQLKKGDNLILSAFGGGFTWGAVYLKWAYNAK"
     gene            complement(36696..38348)
                     /locus_tag="PK252_06315"
     CDS             complement(36696..38348)
                     /locus_tag="PK252_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT14358.1"
                     /translation="MLNKYLVKTDFLSYDDFKKNFEIKVPENYNFAFDTVDEIANEQP
                     DKIAMVWCDDNGAEATFTFSQMKYYSDKAANFFLSAGIKKGDAVMLILKRRYEFWFSI
                     LGLHKIGAIAIPATHLLTKKDIVYRNNAAGIKMIVSVSDSEIVHQIEAALDESPTLKC
                     KAIIGEDKDGWFNFTTELERASSIFVRPTGSEATCNDDVMLLYFTSGTTGMPKMVFHN
                     FTYPLGHILTAKYWQNVQENGLHFTVADTGWAKSAWGKLYGQWLSGSAVMTYDYDKFV
                     PLKLLEIIQKYNVTTFCAPPTIYRFLIKEDLSKFNLSSLKYCVVAGEPLNPEVFKQFY
                     DAVGLKLKEGYGQTECTVALATYSWMEPKPGSMGRPSPGYDIDIVNDNGISCGIGEPG
                     QIVIYTGKSKPVGMFGGYYRDKELTEKAWSDDVYYTGDMAWRDEDGYYWFVGRADDLI
                     KSSGYRIGPFEVESALMEHPAVLECAITAVPDKIRGQIVKATIVTAKGFEHCEALAKE
                     LQEHVKKVTAPYKYPRIVEFVHELPKTISGKIRRVQIRQACN"
     gene            38836..39729
                     /locus_tag="PK252_06320"
     CDS             38836..39729
                     /locus_tag="PK252_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOT14359.1"
                     /translation="MVLFVGNDINNIQSNSNNSWEKLLKRIIEKYGKKVEVDKSQPFP
                     LIYEELYLKLLKTGKLENELTLKEEIAKALSTIKPNEIHRMLLELDCNNYVTTNYDYT
                     LQQAVLNGKTTKKLKNEGLVYEKKFSVFRHHVINNKKFWHIHGEINSPQSINLGFEHY
                     GGQLQHLRDYVVSGVKYKTRENPIAPLYQRLKENDVKNISWVDLFYTDEIHIVGLGLG
                     FEETDLWWLITNRARLKYEKKLDALKNNKIIYYCPLLYKNYHKKQIMDANGIKTIYIN
                     KINTEFYFEVINRIKNKNTIA"
     gene            39958..40326
                     /locus_tag="PK252_06325"
     CDS             39958..40326
                     /locus_tag="PK252_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOT14360.1"
                     /translation="MESDVDKKQDFSIKESEEIYSKAVRAGKRTYFFDVKATKFNELY
                     LTVTESKRKQNQDGSFYFEKHKIFLYKEDFEKFLDGINEAIDYIKTANPIIIKPAENQ
                     LDYPEEQFVSIDFEDLDTKM"
     gene            complement(40377..41288)
                     /locus_tag="PK252_06330"
     CDS             complement(40377..41288)
                     /locus_tag="PK252_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT14361.1"
                     /translation="MKPTLLVLAAGMGSRYGSLKQVDKIGPSGETITDYSIYDAIRAG
                     FGKVVFVIRKSIEADFKEVFQRFEKHIEVEYAFQELDALPSGYASPADRKKPWGTGHA
                     VLVAASKITTPFAVINADDFYGAQSYKLIAEHLIKTANDDKNTYSMVGFELSRTLSEH
                     GHVSRGVCTVNSQSQLEGIVERTKIQRKDGKIVFVESEDKMIPLAENEVVSMNFWGFP
                     VSIFNHLEKQFGDFMKTNKDSATAEFYLPMAVDNIVKAGNAKVKVLRSPDNWFGITYK
                     EDKDMTINRLRDLIGKGIYPSNLWNNK"
     gene            complement(41359..41745)
                     /locus_tag="PK252_06335"
     CDS             complement(41359..41745)
                     /locus_tag="PK252_06335"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14362.1"
                     /translation="MMDNLLIEGTKKTPTVHLKKDGQMLLKGRSIPEDPSKFFDIINS
                     WIEEYSHHPAPVTELNIDLEYFNSGTSKAMLNMFHQFVDLKSKGHQLVIKWHYEAGDD
                     DIKERGEYYSTLLDYPFEFIEIEGNE"
     gene            41885..42427
                     /locus_tag="PK252_06340"
     CDS             41885..42427
                     /locus_tag="PK252_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT14363.1"
                     /translation="MNEKTLIEAALFAAEKHRLQRRKSLLSIPYINHPLAVCYILAQS
                     GISSINVLVAALLHDTIEDTETSPDEIANLFGNEVLKIVLEVTDDKTLNDKKRKELQV
                     IKAPQLSQGAKLLKIADKISNLKDILTHPMDWSNKRKKDYFLWSKQVFDGCKGINPQL
                     DKLFEETYNEGIEKLSSLPD"
     gene            complement(42407..43153)
                     /gene="pssA"
                     /locus_tag="PK252_06345"
     CDS             complement(42407..43153)
                     /gene="pssA"
                     /locus_tag="PK252_06345"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOT14364.1"
                     /translation="MKQIVKNIPNFITLLNLVAGFTAIIFAFNINTIAYAPYYIFLAA
                     VFDFFDGFAARLLKSNSVIGKELDSLADVVSFGIAPGVLMYQMLQFSSFNFLMFEKST
                     FVNIIVASLIPAFSALRLAKFNIDERQGDTFIGLPTPASAILIASITIIFFSSDSLLG
                     LGALSYTYIGLIAISVVDSFLMVCNLPMFSLKMKSFSLKKYSIQILFLFICLLCLILM
                     SIKALPVIIVFYILISVFLYVKERLVGKAA"
     gene            43280..45481
                     /locus_tag="PK252_06350"
     CDS             43280..45481
                     /locus_tag="PK252_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOT14365.1"
                     /translation="MSKSKVFIPEYLAREYRKLLKLLRSTKGDVDFSKIRAAFTLAND
                     ACNCNNSYYSEPLIMQAVQVAQIVANDLPLGTEQVCIALMFKFVENGLLDEKNIEASL
                     GKHYAKQTKSLAEISKINTQKISGQAENFRKLLLTITDDVRIVLVKLADRLYVMRNLE
                     TEETEVQKRIAKEVFDLYAPIAHRLGLYHVKTELEELAMKFTNPDMYNYIEKKLQDTA
                     SARNKFIKEFIVPVKAILDQNNFVYEIKSRTKSIFSIYNKMRKQNIEFEEVYDLFAIR
                     IILKSSLENEKSDCWKVFSLITSMYPHNPDRMRDWITVPKSSGYESLHATVMTEQGKW
                     VEVQIRTQRMDEIAEKGFAAHWKYKGQKADGTIDTWINKIREALESSASDSESAIDNQ
                     KLSLYSQEIFVFTPKGDLRTLPKGATVLDFAYEIHTNVGNTCTGAKINGKNVPIRYEL
                     KNGDKVEIITSKTQTPKNDWIEFAVTTKARSKIKLALKNELILRAEAGKEIFRRRLRN
                     WKIGFNDVLVSKLMKHFKIKTAIDFYSLIADEKIEMTEIKKIITASEKTANESPEKID
                     NAQLDKIIPSLAVKSEEVLEIDNKIINHVEYKLAKCCSPIFGDDIFGFITINDGIKIH
                     RVTCPNARDMVNRFGYRIVKARWSNTDNNTFFQAALRVTGSNELGILSKITDIIAKDL
                     KVNLRSINVDTQDVVFEGVIKLFVKEVSHLDMLIHKLMKVNGVDVVHRIEA"
     gene            complement(45489..45692)
                     /locus_tag="PK252_06355"
     CDS             complement(45489..45692)
                     /locus_tag="PK252_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14366.1"
                     /translation="MQEVSMFTKLIEASKTHLTKGAYQLVYDETHELVQVVHKRNGEV
                     NYEICSDGDEMLMATIIYFGLKN"
     gene            complement(45817..47121)
                     /gene="murF"
                     /locus_tag="PK252_06360"
     CDS             complement(45817..47121)
                     /gene="murF"
                     /locus_tag="PK252_06360"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT14367.1"
                     /translation="MNINDLYLIYQQYPLISTDSRNIIPKSIFFALKGESFDGNKYAF
                     EALDKGAAYAVVDDVKVNHPKCFLVENVLNTLQELANYHRKHLNTKVIGITGTNGKTT
                     TKELTLATLSKKFKTIATKGNLNNHIGVPLTLLSVPVDTEMAIIEMGANHIGEIAQLC
                     SIAEPDLGIINNIGKAHLEGFGSFEGVVTAKTEMYRYLNNKENGIIFYNANNPLLVSE
                     VAKVSCQKIGFGDAAKTEIYGEIVEVNPTLVCKIYCKGSERIVKTHLTGSYNLENILA
                     AITIAMYVGVAFADACEAIAEYVPQNNRSQIVEKSFNTVLCDYYNANPVSVEAAIRNF
                     AILKTGKREKKIVILGEMKELGKYSLEEHTKIVNLLSEMKFSEVYLVGNSFIETAKQV
                     NFKTFENTNALVDYICQNPIKDAMVLVKGSRAMKLELTVDVL"
     gene            complement(47211..>48436)
                     /locus_tag="PK252_06365"
     CDS             complement(47211..>48436)
                     /locus_tag="PK252_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT14368.1"
                     /translation="VSVSSFYLDETEVRNVDYREYLFWLKRVFAEYPAVYKNALPDTL
                     VWRSKLAYNEPYVEYYFRHPAYNQYPVVGVNWIQANDYCAWRSDRVNEKIMIDNGYLK
                     MDPQQSGAENFNTDAYLVGQYDGLPGENPKLSPMGVERKITLEDGLLLPKYRLPTEAE
                     WEFAAFSLIGNSFDERIYERRLFPWNGHTLRNDDAKSRGQMRANFVRGRGDYMGTAGD
                     LNDNADVPAPVKSYWPNDYGLYCMAGNVSEWVMDVYRPLTQEDNDEFRPFRGNVFMEM
                     ERDNDGNVAPKDSLGRIKFKPVDPANAANRRNYKQADYRNYLDGDVQSSLDFSTSTTS
                     AQNPGGSTRMYAQGQGDMSSLITDNVRVYKGGSWKDRAYWLVPGTRRFLNETDSRDDI
                     GFRCAMTRVGSPKGF"
BASE COUNT        16066 a         9589 c         8245 g        14536 t
ORIGIN      
        1 cttatttata attaataata ggtaaaattt tgatgaaatg ttgaaaatta ttgataataa
       61 acaacattcg tgttgacatt aaaacatttt ttctatattt ttgtaactat tgctaaaaca
      121 ccttaaatat ggaaaatctc gaaatccaag gcacaaatga tactccttac attttttgtg
      181 atgcagaaaa aaaggaaata acccttacag gacggtcatt accagaaaac ccttttaaaa
      241 tatatgaaga agtgtttaaa tggggcgaac aatatgacgg cgaaagcatt cagataaatt
      301 ttaagctgga atatttcaat acagcctctt caaaacaaat ctttacctta ataaaaactt
      361 ttgtagaaaa taaaactgtt aaagaaataa aagtaaaatg gcactacgaa gaaggagatt
      421 atgacagcaa agaaacaggc gaacattttt cacatttatt tcgtattcca tttgatttta
      481 ttgagtatgc cgaaagtgga ttctgatatt taaccctgcc tgagcagggt ttattttttt
      541 gttagataaa acaatttttt taaaaattac caacataatt tttttttaat aaaaacatta
      601 ttaagtttgt acagctatta aatagccaat tattttcaaa ccaatatttt ataaactatg
      661 agaaaagtac attacagcaa attagcagta ttactgtctt tagcagttgt tcttggcagt
      721 tgcagcggac taaaaaagat gcaggaacaa gcatctacta ctaaatttga gttaacacca
      781 aaagtgttag aaatgcataa tggagaagtt aacgcaacca ttagcggaaa atttccacca
      841 aaatacttta acaagaaggc tgtagtaaca gtaacgccta ttattaaata cgaaggagga
      901 caaattgaac ttcagtctta taaacttcag ggtgaagacg ttcaggctaa tgacaaagtt
      961 ataccttttg aagctggtgg agatttcagc tatagcgaca aatttgcata caaagacgaa
     1021 atgatgcttt caaaagtaag cgtacaagta tcagctcaaa tgaaaaaaga aaaccttgtt
     1081 ataggaactt ttgaaattgg agatggtata atagtaacgc ccaaactggt ccgtctcgaa
     1141 cctaaagctt cagagcttac tcaaaatcaa tttgtaagaa atgtagccca agacaaactg
     1201 gcaaatatta agtatttgat caaccaggct gaaatccgca atacagagct taagaaagat
     1261 gaagtaaaac aattgaacga ttttgtaaaa acaattgcaa aagatcctaa atttaacatt
     1321 aaaggcatcg agctttcatc ttatgcttct cctgatggtc ctgaaaataa aaatgcagaa
     1381 cttgctggta aacgtgaaag tgcagctgta aagtatttta aatcagagct taagaaagca
     1441 aaattagata aaatcttaaa agattcttta ttctctatgt tgaaaacccc ggaagactgg
     1501 gatggcttca aaacatcatt agaaaaatct gatgtaaaag acaaagacct tatcttacga
     1561 gtactttcta tgtattcaga ccctgctgta cgtgaaaaag aaattaggaa tatttcacaa
     1621 gcatttgatg agttaaaagt aaaaattctt cctgagcttc gtcgttcaaa gcttatagta
     1681 aaagctgaag ctattgggcg ttcagacgaa gaattgatta gtttagctga ttcaaaccct
     1741 gatgctttaa gcctcgaaga agtcgtttat acagctaaca tgatccaaga ccttgataaa
     1801 aaagctgcct tatatgcaaa agcttcagaa aaatatccta atgatgtacg tttaaaaaac
     1861 aaccttggtt atgtatattt caagcaaggc aaacttgacg aagctaaagc tgctttagaa
     1921 caagctaaat ctatagaaaa taacgatgct gttaaaaata atttaggtgc aatagcttta
     1981 atcaaaggtg atgtagctgc cgctgaagaa ttatttaccg ctgcccttgg agctggcgca
     2041 gcacctaatt ataaccttgg tattgttaat attatcaaag gtaaatatga catagcagta
     2101 tcatattttg gcaacgaatg tgatgccaat gcagcacttg ccaaattgtt gaacaaaaac
     2161 tacgaagctg ctcaaaatgc catgggctgc agcaaagacg aaagtgccca gggatattac
     2221 ttaagagctg ttattggtgc ccgcatggac aattcagact tagtattcaa caaccttcgt
     2281 gctgctgttg gtaaagacat ccaacttaaa aactatgcca agaaagatgt tgaattctta
     2341 aaatacttta acgacgaaac tttcaaatct attatccaat aataatttga ataaatgata
     2401 aaagaaaagc tggccaaatg gtcagctttt ttttgtttta tatcatcaaa aaaattaacg
     2461 cgaaagctga gatacaattt tgaccgtttc tacagcttct ttgacatcat ggacgcgaag
     2521 gattttagca cctttcaaaa gggcaatcgt attcaaggcc gtggttccat taatggcttg
     2581 ttcaggatta gtttgtagta acctataaat catagattta cgggaaatac ctactaaaac
     2641 aggcaaatcg aaaaactgaa aaacctcaag tttattaagc aactgatagt tttgttccaa
     2701 tgttttagca aaaccaaagc ccgggtcaat aataatatca gatactccca tttcctttag
     2761 tttatctacc ttctcggaca actcaaaaat gacttctttg acaacatcat tatacacagc
     2821 ataatcgttc atatttgaag gattgccacg catgtgcatc agaatataag gcacctttaa
     2881 tgatgcaact gtctgaaaca tcttttcatc caacgttcca cctgagatat cattaacaat
     2941 accaatgtta tacttttcta ccgtccatct ggctatttca gccctaaagg tatctaaaga
     3001 tattattacc tcagggaagt tagccctgat aacggttaat gcaactttaa gcctggcctt
     3061 ttctgttgct tcatcaacat attctgctcc agggcgggtt gaatatgccc ctatatcaac
     3121 aattgagcct ccttcatcaa taatttgttg cgttctgtct atgatttctt tttcagtaac
     3181 aaatcgactc cccgaaaaaa aagaatctgg cgtgatattg acaatgccca tgacaagcgg
     3241 atggtctatt tcaaccaaag agccatttac attgatagta tatttattag aaatcataaa
     3301 acactactta ggcactttgc gataaaacca gtaagcaata atggcataaa taatattggg
     3361 taaccacaca gcaagcaaag gacttaacga gccacttata gcaaactgtt gagaaaactg
     3421 cataaacaca atataactaa agcaaagccc catgcctata ccaatatgaa cccctgtgcc
     3481 tccacgggtt ttccgggatg aaacacaaac cccaataata gttagtatat aagtagaaaa
     3541 aggtaaagaa acccttttgt acctctcaat ctgataggct tctatgtttt cgccaccttg
     3601 taactcctgc tcttcgataa aagcattaag ttcaaaaagg tccatagatt caaccacgtt
     3661 gacccgacgc ttaaaatctc cgggataaat atacagcgtt gtatcaattg ctttgccttt
     3721 ggttattttc tcattcaagc tatcaatagt cctgatgtaa tagtttctta tcgtccattt
     3781 acttttggtt gagtcccagg tcataaaatc tcccattaac ttagatttaa gcgtaccatt
     3841 ttcaaaattt tcaatagaga ctttatagcc ggtattggca ctattggagt atgattccat
     3901 ataaatcacc ataccaggcc taacctgctt atggatattg cgttgattgt attgaaaagg
     3961 gctgttatga atgtaacggt cttcaaactc gaggcgaacc ttattggcat tgggaattac
     4021 aaaattcatc aacaaatagc tcataatggc aataaacgtg gcgccccaca tataaggcac
     4081 caacattcgt ttgtagctta ccccgctgct caagatggca ataatttcag tcctgtaagc
     4141 caattttgaa gtgaaaaaca caacagaaat aaatgtaaac aagtgactga aaagtattgt
     4201 aaaataaggg ataaaattca ggtaataatc aaaaatgatg gcctgcatag gcgtcttagt
     4261 ctctacaaag tcgtctattt tttcggccac gtcaaaaaca atggcaacgg acatcaccaa
     4321 gataatagaa tatacaaacg tcccaagaaa ctttttgaga atataacggt caatctgctt
     4381 aaaacctatt ttataggtaa attcattgaa ttgaactaac catttctgta atatagattt
     4441 tatcgacttc aaatgacaat ttattatata aattacctgg gacgcatgtt ttttaatgag
     4501 ttggcaaatt tggctgcatt gccgccactc atcatcttca tcatcttgcg ggtgtcttca
     4561 aactgcttaa gcagcctgtt aacctcctgg atattagttc cactgccatc tgcaatacgc
     4621 ttacgacggc ttccgttaat caaatcaggg ttttgacgct cttttggggt catagactta
     4681 ataatagctt caactccttt gaaagcatta tcgtcaatat ccatattttt caaggcttta
     4741 ccaacacccg gaatcataga agccaggtct ttgatgttac ccatcttttt aatctgctgt
     4801 atctggccta aaaagtcgtc aaagtcaaat tggtttttgg caatcttctt ttgcagcttg
     4861 cgggcttctt ctgcattaaa ctgttcctgg gctttttcaa ccagtgtaac gatgtcaccc
     4921 ataccaagga tacggtcggc catacgatca gggtagaaga tgtcaatggc ctccatcttt
     4981 tcgccggtac tgacaaactt gataggtttg ttcacctcag attttacagt aagggcagca
     5041 ccaccacggg tatcaccgtc taacttggta agtacaacgc catcaaagtt gagcctgtcg
     5101 ttgaatgcac gtgcagtgtt tacggcatcc tgaccggtca tggagtcaac cacgaacaat
     5161 atttcgttag ggtcaacggc ctttttgata gcagcaattt cgttcatcat cgcctcgtca
     5221 acagacaaac ggccagcggt atcgatgatt acaacattgt gtccatgttg ttttgcatgt
     5281 tttatggcac tctgggcaat gttgaccggg ttggtattat tttcttcggt ataaacctga
     5341 acgccaacct ggttacccag caccttcaac tgttcgatag cagcaggacg ataaacgtcg
     5401 ccagcaacaa gcaatacgct cttacctttt ttggtacgca agtaattagc cagcttgccc
     5461 gagaatgttg ttttaccaga cccctgaaga ccagcaataa ggataataga agggctgcct
     5521 ttcaggttga tatcctgata agcaccaccc ataagctcta caagctcatc gtgaactatc
     5581 ttaacaagca attggcctgg tttaagtgaa gttaacacct cttgtcccag cgctttttgc
     5641 ttaacagtat cggtaaagtt tttagcaatt ttatagctta cgtccgcatc gagcagcgct
     5701 ttacgaactt ctttgagcgt ttcagcaaca tttatttcgg taatcctacc ttcgccacga
     5761 attcttttaa aggatttctc cagtttctcg gataaattct caaacatggt tttacaattt
     5821 tgactgcaaa attaaattta ttcttcaaat tcgctatgtt tacattagtt aatagttgtt
     5881 agttggaagt tagagtcgtt tatagcaaaa attctggtaa atttaagaac gaacgtaatt
     5941 tgatttatgt tatatagatt tgtaacaagt ttaataaaag atatatacat tttttgtttt
     6001 tgatgttagt taatattgtt ataattcttt acaaaagatc aatttgcaga gattaaaaaa
     6061 tacggcacct aaataacggg agctaaacat actgtattta aatagattgc gagtgtctta
     6121 ttccgcaatg agcttcacca aaggtacaga aacctaacac acaatatagc ccaataaggg
     6181 ttggttggta tgcgatggtt tgtagcatgc tttaatttat gtgggccatg atagaaaata
     6241 gccccgtaat ccggtacgac accacaccat caacgtttca tcaaaatggc acaatgttaa
     6301 acattttagc ctccaacgtg gcaattattt atcccaaacc aatctgaaaa atagttagaa
     6361 aaggggcttt tttacccatt caactggtgt aaagccgcaa actcttctat ttcctttttg
     6421 ctattcctgg ttttgtgaat ttccttttga ttggcgatat actctcctac tttctgcgcc
     6481 tgcgaatgac ttaccgaata tacgtacacc tggctatgcc aggcaaatga accagcttta
     6541 aaatgtttct cattaatgac agcagcactt tccaatgcaa atgattgtac attgaaggaa
     6601 acagaagatt tatcctcatc caataccagc aaatgaatat ggtcaggcgt tccgccaatt
     6661 tcacttacct gtaaacttac atttttgcaa cattcacgta atgtagcata tacatctttt
     6721 tccatttcag gaagtatcag aggcagcctg ttattggttt gcagcacaat gtgcaaccac
     6781 acctttttaa tgatgctaaa ttcagtcata catacttaca aaatagtgag tatttccaca
     6841 aaattcaata aaacgcataa aatgaggcat aggaataacc aacgatgccg tattaataca
     6901 aggatgaaaa ctaagttttg aggcctcttt aagcttctcg tcaaagaaaa ctgttacatg
     6961 tttttcggta tcggcaaaca acgccaaggg cgaaacagaa cctggcttga cctgcaaata
     7021 cttccacatt cgttgttccg aggcaaaagt cagtttccct tgtttcaaac gcttttccaa
     7081 atcatgtata ttcagtttat attgataatg caacaccacc aggtaatgcc tgtcgccttt
     7141 atggtttctg aaaaacaagt ttttacaatg cgttgcttct atgtctgccc aatatttctt
     7201 agcttcttca atagttggag ctgccggatg ttcgtaatag tcaaaactaa cctgcaattg
     7261 gtcgagaagt tgatataatt cagcagggcc attcatggtt ttactttttt agaataacca
     7321 tagtggctcc aaaaccatat tcagcatagg atgcatcttg gtattgcaat gaagggtatt
     7381 tatttttcaa tactttctga atttcgtacc tgagttttcc atttccaaca ccgtgtatat
     7441 aaacaatacg tttggtttta gccttcaaag ccccttccaa cgtagtctca aacctggcca
     7501 tttgcatttg cagtatttcg ccaggctgta aaccttcaat actatcagta atttcttcaa
     7561 tatgcaagtc cacttcctcc gtttcattta ccacttgggc ttcagactca atctttacga
     7621 catctttatt ggtttttgaa gaagatttaa acgatgtctt acggcctaat tggataataa
     7681 ttgcaggctc atcaaaatag tcatttttac tgaatgtttc agggttattc caagcaatac
     7741 cagcaaaatc ggaattatgc gtagttggaa cctttgaaat gtacgaagat gttgaaaaat
     7801 ataaaacatc taccactatt cgctggatag ttttgagttt ctcaagggta tattgcccaa
     7861 tgttcatgct catgccagct tcaagttcac ccttttcaat taacctatat atattatcct
     7921 ttacttgtgc tgctgtaaat aggatatcat aattactgtc gttcagaatg tataaatcga
     7981 aaaggctgta tgcaaaagca ctatcgaggc caacaggcac tatagccaga cataccaatg
     8041 ccttgtcatc ggatactatt ttcttttcag aaaatgtagg cccagaagct ttatcctgag
     8101 gttctttaac aaaattttta ggcttttcaa cttttgttgg tttggcttca ggctctacat
     8161 ccaatgcaat gtgttgcgca gaggtgtcat gtaccaggtt ggaaagcaaa gtattgattt
     8221 caaaaccatc ttcaatctcc accatagcct gattatttgc caaaaatttt ttaatcaccc
     8281 cgccaccagc ttcgtttaga aacttaacct tatctcctat tctaaatccc attgtttcgt
     8341 tttttagcaa aggtaagaaa ttgagatttc tcaaataata attttataaa ataacaccca
     8401 atgtcgttta gttaagactc tgacaggttc tgagaacact gtcaggtcag atgtgatact
     8461 aacgccgaca gcttctttca agcctgtcgg cgttatagta gaatcgttga caaaacgaca
     8521 ttaaaataac ccctctaata attcgttgat gttctttttg ttaaattttc ttttaaaaaa
     8581 gacaaaatac attctttcta tttatcagga agaaataatt ttgcgctctt aaattttaca
     8641 cctataattt atttattgtg agtttacaag acgaaatcaa aagaagacgg acctttgcta
     8701 tcatcagtca tccagatgcc ggtaaaacaa ccctgaccga aaagcttttg ctctttggcg
     8761 gggctatcca cgttgcggga gcagttaaat caaataaaat caagaaaact gccatgagcg
     8821 atttcatgga gatagagaaa caacgtggta tatctgtttc aacttctgtg atgggctttg
     8881 aatataaagg aaaaaaaata aacattctcg atactcccgg tcacgaagac tttgccgaag
     8941 acacctaccg tacactcact gctgtggata gtgttattat tgtcattgac agcgccaaag
     9001 gggtcgagac acaaacccgc cgattgatgg atgtttgccg tatgcgcagc accccggtaa
     9061 tgatttttgt caacaaactc gaccgccccg gtaaagaccc ttttgaactg cttgacgaaa
     9121 tacaggaaga acttaagatc catgtgcgcc cgttaagctg gcctatcagc aatggctcaa
     9181 cttttaaagg ggtttacaat atttatgaaa agaatctcaa tctttttgag ggagctgaca
     9241 agacaaccat agaagaagcc atagaattta aagatattga aaacccggaa ttggaaaact
     9301 atattggcga gaagtacgcc aaaacattac gtgaagaact tgaattagta gagggagttt
     9361 acgaaccttt tgaccatgaa ttatattcag aaggccacct tgccccggtt ttctttggca
     9421 gtgctttgaa taatttcgga gtccgcgagc tactcgactg ttttattgaa atagcgccat
     9481 ctccaaaagg ttttactact gtcgaacgca gcgttgatcc ttatgaacaa aaattcaccg
     9541 gctttgtgtt taagatacat gccaacatgg accctaacca ccgcgaccgt attgcatttg
     9601 tcaggatatg tagcggaaca tttatgcgta ataccaatta tctgcatgtt cgacagggca
     9661 aacaaatgaa attctccagt ccgacagctt ttatggccga caaaaaatcc attatagacg
     9721 aagccttccc gggcgatgtt attggtttgc atgacaccgg caacttcaaa attggcgata
     9781 cgcttaccga aggagaaatc cttcatttca aaggtattcc aagcttttcg cccgagttgt
     9841 ttaagtatgt tgaaaatgca gaccctctaa aatcgaagca attggccaaa ggtttggagc
     9901 aattgatgga cgaaggtgtt gcccagcttt ttatcaacaa atcgaatggc cgtaaaatta
     9961 ttggtacagt aggagcatta caatttgagg ttttgcaaca cagattatta catgagtacg
    10021 gagcttcttg tcgttacgaa ccaatcaact tgtacaaagc ctgttggatg gttagttcaa
    10081 acaatgccca gttggaagat ttcaaaaaac gtaagtatca atacattgct gaagataaat
    10141 atggccgcga cgtattttta gccgattcgt cttacgcttt gtctatggcc caggaaaaat
    10201 ttcctgacat caagtttcac ttcacttctg aattttaaaa attgtttaat attttatatc
    10261 tcaccattca tttttgatta taatttatta aacgtaagtt aagactaaac aaaaggctct
    10321 tttacctttt gtttagtctt agttttctgg tacaactcaa attaacactt atttataagc
    10381 acttcaacat gctgatacat aaaggcctga tgtttttttt catagcaaag aaaatatatt
    10441 tgcaaaaaaa taaactaaga aaacatgaag aaaatagaag ctatcattcg taagtcgaaa
    10501 tttgatgatg taaaaattgc attacaaacc attgatatcc aattttttac ttattgggac
    10561 gtaacaggcg ttggtaaaga aactgaagga catatataca gaggagctgt atatcaggcc
    10621 aaccaaatac agcgcaccat gttaagcatt gttgtccgtg accacaacgc ccaaaagact
    10681 gttgatgcta tcattgctgc agcacgcacc ggacaatttg gtgatggcaa aatctttgtt
    10741 tcggacattg aagaagtaat ccgtatccgt accggcgaaa aaggccctga agcactgtac
    10801 ctgaaggaag aagaaaaacc tttagaaatt aagtaaacca cttattaatt actatatagt
    10861 acagctgtat tgcgcactaa tacagctttt ttgtttaccc ataaaaaaag cgcctatacc
    10921 taacagatat aagcgctttt ctaattttat ttcctattaa aaaggaaggt cgtctattgg
    10981 agattcactc acctgaaaag gttcttcctg aggctttgca acagtaccag cacttgtctg
    11041 ggctccacta ttattaccat cttctggttt gcggcctaac aactgaacgg tatcggctac
    11101 aatctcggta atatatcgtt tattgccatc tttatcgtca taagaacggc tgcggatttt
    11161 cccttcaaca taaatctgag aacctttgcg gatatatttt tcagccagat cggccaacga
    11221 acgccacaac acaacgttgt gccattcggt agtgcttacc ttttcaccgc ttttattggt
    11281 ataatattca cttgtggcca acgctatggt acttacggct gttccatttt caagatgcct
    11341 aacttcaggg tctttaccca cgttgccaac taaaatcact ttgtttactg acataataat
    11401 tgttttaaag gttgttaaat atttttcact ggtgaaaata gtatttttaa tccttaaagt
    11461 caaattcttt ttccatatat cgctcgatca cttttggaac aggatattgg tgaaactggc
    11521 tttttggtat gcaaatatat tccgaaagtt catttggcaa tgccgacaat tcaatatgat
    11581 aaaaagttgc aactaccgtc cgatgagtaa gttgatgctt aaccgcatct gaatgcttct
    11641 ttatattcca ctggatatca ccaacatatt cgtttattat tttttcaaaa tatttttttt
    11701 caacagtcaa caattctttt gtttctgaca ttggaaactc gtacagcccc ttccagatat
    11761 cctcattgtc tctttttcgt aaaaagaaac tttgcttgta ctgaaaaata aagtaattga
    11821 aaaatcggga tgttggcgct ttcttttgct tttttacagg gaaactaagc atttgatttg
    11881 tttgaaatgc catacaaaca gacattatag ggcatccggc acaatccggg ttacggggca
    11941 aacataccag cgccccaaac tccatcaaag cctggttgaa atcagaagga gaacaatcaa
    12001 ccatcataga ttgagctatc ttgtaaaagt gggtatagcc attgtcatca tcaatgcagg
    12061 tactatctgc aaacaaccgc gacaaaaccc tgaaaacatt gccatcaatg gcaggcaccc
    12121 tttctgcaaa agctatagaa gcaattgccg cagcagtgta acggccaata ccttttattt
    12181 tgatgagttc agcatagcta ttgggcattt tacccccgaa attattaaca atctgcattg
    12241 ccccggcatg aaggttccgt gcacgggaat aataaccaag cccttgccaa cacttcatta
    12301 cctcatcaat attggcattg gccaatgaaa caacatccgg aaactgatca ataaacctgt
    12361 aataataatt tatcccctga ttgacccttg tttgctgaag aataatttct gaaatccaaa
    12421 tgtaataagc attatttgtg tcccgccacg gcaaatccct tttattttga gcaaaccaac
    12481 taattataga ctttttaaaa aatgtaaatt catgcatacc aacaaaataa aaatcaactt
    12541 tgacaaaaat aatgcattgg catgttcaat tttaaaatta aaacttatat atttgcactc
    12601 ctaaaatgaa tacattatta gttaaaatat tgataattaa aggttttaag aaaaatgaca
    12661 aaagcagaaa ttgtaaatga aatctcgaaa gttaccggga ttgaaaaagt tactgttcag
    12721 aaaacagttg aagcttttat ggaaagcata aaagattcta tggtaaaaga taaaaatatt
    12781 tatttgagag gttttggaag tttcattgta aaaaaacgtg ctcaaaaaac agcaaggaat
    12841 atctcaaaaa ataccaccat tgtaattcca gcccacaata ttccggcatt taagcctgct
    12901 aaatcatttg taaacaaagt taaatcgagc attaaataat aaaaatatta atcctttaaa
    12961 gagttaaaaa catgccttgc ggaaagaaaa gaaaaagaca taaaatggcg acccacaaac
    13021 gtaaaaaacg cttaagaaaa aatcgtcata aaaagaaaaa gtaattagta ttcttttact
    13081 aagctattaa taaacctaaa atcctaaaaa acatctggat tttaggttta ttggtattgt
    13141 acaatataca actcaacaat attttcaact tattaaaaat attgtttaaa aaataaaaga
    13201 ttgtggatac aaataatgaa ttaataattc atgtaaccga atcagacatt gatattgcca
    13261 tattagaaaa cagacagctt gtagaacttc ataaagaaaa aaataacaat cagttttctg
    13321 ttggagatat ttacctgggc aggataaaaa aaataatgcc cggtttaaat gctgcatttg
    13381 ttgatgttgg ctctgaaaaa gatgcatttt tgcattatca cgacttaggg ccacaggtgc
    13441 tttcgcttaa caagtaccta aaacaaagcc tttcaaaaaa agggaagcct atatcattgt
    13501 ctaaattcga aaatgaatta gatattaaca aaaacggtaa aattgccgat gtccttcaac
    13561 aaggacaaat ggtattggtg caaatagtaa aagaaccaat atctaccaaa ggcccaagac
    13621 taagctccga actttcactt gccggacgca accttgtttt gatgcctttt tctgacaaaa
    13681 tatctgtctc acaaaagatt gaatctgctg aagaacgcaa caggctgagg actttaattc
    13741 aaagcatcaa gccaaaaaac tttggggtta tcattcgcac tgtggcccag gataaaaaag
    13801 tggcgacatt tgatttagaa cttcgcagtt tggtcaaaaa gtgggaatcg gccttcgaaa
    13861 atatcaaagg gacaacaacc cccaaacgag taattagcga agtaagcagg acttcagcta
    13921 ttttaagaga tttaattaac gcttctttca gcaatattta tgttgatgat gaggctacct
    13981 gtcaggaaat aaaagaatac ttacaagaaa ttgcccctga aaaagcagat atagttaagt
    14041 tctacaatga atctacccct atttttgagc atttcaatat tgaaaaagct ataaagcttt
    14101 tgtttgggcg cacagtatca gtcagaagcg gtgcatacct tgttattgaa cataccgagg
    14161 ctttacatgt tattgatgtt aatagtggca acaggtcgcg agctgcaaaa gaccaagaaa
    14221 ccaatgccct tgaagttaat ttagctgcat gcgatgaaat tgcccgtcag ttaagactta
    14281 gagatatggg aggtattata gttgttgatt tcatagatat gcaaagcaac gaccacaagc
    14341 aacaggtgta tgataaaatg aaaaacctgc ttgccgagca gaaagccaag cataatgtcc
    14401 ttcctctcag caaatttggg ttgatgcaaa ttacccgcca gcgtgtaagg cccgaaacaa
    14461 acatagacac aaacgaaaaa tgcccaacct gcaaaggtac cggagagatt tcgtcaagcg
    14521 tattgtttga aaatgaactg gatattgccc tgagcaaata ttttgaaaaa tcaaaaaata
    14581 agaaaattac cctgagggta cacccataca ttgcagctta tttgaagaaa ggcttccctt
    14641 caatccgaat ggtatggagc ttaaggtaca aatgttttat taaagttgaa gccgcttttg
    14701 ctttaacttt ctttcagtat aaaattgacc cgcctgctga gagttaaaga agaaacacta
    14761 caaacaaagg cataagctga ttaacaacgg tttatgcctt ttttagttgt tcccaattca
    14821 tacatataaa gtcttcaaaa cttcagaaat gtttgcatat tttcatttag tccttaataa
    14881 atacagtaaa taggctattg tacaattact atattttcag taatattgta ttatgatatg
    14941 ttattgacat aaaaatacta tctaacagcc atagcatgaa gcatccatga ttgaaaatac
    15001 gaagattttt atatgcagtt cttcgtctta gacaagacaa gccatcattc tattaaaatt
    15061 aaaaatatga taaaaaaaat catcacgctc atctttacac tattttctgc tttcatcata
    15121 gcaaacgggc aaaacaatat aaccattaca ggtaatgtaa ccagtgctga agataacctt
    15181 ggcgttattg gggctgcggt atatgttaaa ggtactacca ttggaactac taccgatttg
    15241 gatggcaact ataagctaac cgttccggag ggtaccaaaa cacttgtatt tagttatgtc
    15301 gggcttgctt cacaagaaat tgccattgaa ggccttactc aaatcaacgt agtgctaaaa
    15361 cctgatgtat ttaagatgga cgaagtagtt gtagcgggtg ttgccagcgc tactcccaaa
    15421 cgcaaactca gtgtatctgt agctaaagta ggagcagatg atttacaaaa tgttccggcc
    15481 agctcggcat ctacagccct ccagggcaaa gtggcaggca ttaccgttgt tggaagtggt
    15541 tctccaggtc agggggccag catcaggctg agaagctcta catctatcta tggctcacaa
    15601 tcaccattga ttattgtaga tggggttatg attgacggag accttgccga tatcaatgca
    15661 gatgatattg aaactattga agtagtgaaa ggggctgcag cttcggcgtt atatggctct
    15721 cgtgccggga gtggagttat ttccatcaag acaaaacgtg gaagctcggc tgctgaagga
    15781 accacagaaa taaaatttcg taatgaattt ggcttcagcc agcttgcccg taaaataaaa
    15841 cttgcagaac atcatccata tcttgttgga gctgattaca accagggcac ttataccaaa
    15901 tacgacagtg taacatatcc tgtcggctat gtccaaggat atgacaaccg catcaaaggc
    15961 agccgtatgc tggattatga ccattatgcc gacaaccctt atgcttttgt aaaagaccct
    16021 caggatgaga tgttccaaaa cggtttgttt tacaccaatt acctgtccat agcttcaaat
    16081 agcaataaaa ccgggatact tatttctttt gaaaataata aaaacaccgg tattgttttt
    16141 cacaaaaaag gccaaagccg ccagaatgta aggatcaatg ttgaccataa actaaatgat
    16201 attttcaaaa tatctgccag ctcgctcttt acccaaaatg aaatagacat gtccactact
    16261 gatgcttata cattaggagc cttttcagat gtattattta tgaacccgga cgtaaacctc
    16321 aatatggatg ctcctgcttc tgacactact attcttaaaa aatattacat aaagccagac
    16381 aactgggcaa taaccggcaa ccccaaacat accctgtatt atgaggaagt aaccgaaaaa
    16441 cgcaacggga tgctgctcaa cctgaacgga agcgccaact tattgtcatg gttggttttt
    16501 gatgcagaat atagtctgga aaaaaggttc acttattatt cgcgttttgt cccgattggc
    16561 tttttgggaa gtaataaaga aaatgaaaaa aacggttcta ttaccaaaag tactctccct
    16621 cagacaagcc aatctgtgca agctactttg aattttaata aaaaatttaa tgaacttgta
    16681 ttaaaaggga aagcttctta tctgtttgaa aaacaacaac aagagtattt ctatggagca
    16741 ggagactcat tgcttgccaa tggggttaag tccctttcag cattgggcaa aggaatcatt
    16801 gttggttctg acgttattga tatcagggca agagattact ttggaatatt agacttagat
    16861 tacaaagaca ggtatatcgc ctctattctt taccgatacg acggttcttc cttatttgga
    16921 gacgaaaaca aatggaaccc ttattacagg gtatcattgg catacagact tagtgaagat
    16981 ttcaaaatac ctaatgttca ggaattgaaa ctacggtact caataggtac ttcaggccaa
    17041 aggcccggat atagctatca atacgaaact tacaatatcg aaaatggcca atatgtacca
    17101 tatagcgctg ccaataaagc aataaaacct tctgaaacca aagaaacaga gttaggtttt
    17161 aacatgcagt ttttcgagcg atttgaagct gattttactt attcgttcaa taaaactaca
    17221 aacgatttcc ttcctgtacc tcagggagcc gcatcgggct ttaaaatccg ctggcaaaat
    17281 gctgccacaa tcgaaggcaa ttctatggaa tttacgttaa gtacacaggc tataaagacc
    17341 aaagactttg aatggcgttt gagcctgtct tatgataaag taagacagaa aatagctgat
    17401 ttatatggtt ctacaccaaa cttttttacc gggcctaata gttctttcca atatttttac
    17461 aaccgcgaag gcgaaaatta tggtgtcatg tatggatatg agtgggtgaa aagcctggaa
    17521 caaatgaaaa accagctaaa agcagatgat aatattgata attatactgt caatagcgat
    17581 ggatatgtga taaaaaaagg aacagaaggt actgtatatg aaaagcccat agctttaaaa
    17641 gatgcttcag gaagtgaagc ttttgtcaaa attgctgata tgaacgccga tttcaacatg
    17701 tccctaaaca gtactttttc ttataaaaaa attactttat cgatgttgtg gagctggaaa
    17761 caaggaggcg acatctacaa ttatacaaag caaaacctgt ttcttgacaa acgtgccgga
    17821 gattacgacc aagcctcaaa accagactat cagaaaaaag ctatagacta ttacaatact
    17881 ttttatgatg gatttaaagc caattcttac tttgtcgaag atggctcata tatcaaactt
    17941 agggaactct cactgtatta caatgttgga aatatcaaaa tatcaggaaa aacaattttt
    18001 aaagcaatga agattggcct ccttggccga aacctgctca cattcaccaa atactctggc
    18061 tgggatcctg aagttgcttc aggtacagac cgcaccaatt tcatcgttga tatgttcaat
    18121 tatcccaact tcaggaccta tacggcctca attgagttga acttttaaaa actgataaaa
    18181 tacgagccat gacaatatat tgcaaaaata ccatatcaaa agcatttcag gtaatagctt
    18241 tagcccttac attaacagca tgcgaaagcc ttgatgtaga aaatacagat gctcctgaca
    18301 gaaaagactt actcaatagc agccgcgact ttggaggatt tgcccaaaat atatatgcaa
    18361 catactggca agccattcat aatgaagctt tttcaagccc acaaatagcc agccttgctg
    18421 ctgccgatca gattactgcc agttggtaca cgtttggctg ctatgagctt tcgcaagaac
    18481 cgcgaattga atggaacaac accccaaact acgagtataa agaaatcact tatacattct
    18541 ttacccgaat gtacacagtg ctttttatgt ctaataccac tataaacttt cttttacagg
    18601 gaaaaaccat cagttcaaat aataaaacca actcgggcta cttaagctat gcctacatga
    18661 tgcagggaat agcccttggc caacttgccc ttacctttga ctcaagtcaa atagttaaag
    18721 aaactaccaa aattgaaaac cttgcgttca gcccttacaa cgaagtaatg gattcagcct
    18781 tagaatcact cgaaaaagcc ataagcatag cagacacaac tacatttaac ttgggctcaa
    18841 atattgttaa tggcgtaacc cttgacaata aattacttag ccgggtttgt cattcattca
    18901 ttgccaggtt ccttactctt aatgcccgca ataaatcaga atatgctgca accgactggc
    18961 caagggtgct tgccaatgcc aaaaaaggta ttaacgaaga ctttgggcca cagggagatg
    19021 gcaataataa atggatagac gaaattgcca ggttcattac caatgttgac aactctcaat
    19081 tgttcttttg caaaacagat aacaggatta tcaaccttct tgacccatcg tacccaagca
    19141 gatactggaa aaatggccat gccaaagttg tacaccctgg ttatcaatca ggagaagcta
    19201 tttcaaaaga ctacagactt acatcagatt ttgaatatag ccctacgata aggttttatg
    19261 cagatagggg tactgttcac tactcgcatt acaggttcaa aaggtttgac aacatccgca
    19321 aaacaggatt gggtaaacta attgaatatc ctgaatacga aaataaactc tatgccatag
    19381 aagctatggc caatatggga caaaccgaaa atgcaataaa cgccatcaat aacattgctt
    19441 accctcgccg taccagagga aaattatctc ctgttgacat aactacctct aaaaaagaat
    19501 tgttagaaat tatattctac gagcgtgaca tcgaattatt tggtcaaggc tatttgattg
    19561 ctttttgcga tatgcgccgc agagataaac ttcaaaaagg cactccccta cattttccgg
    19621 taccagcaaa cgagcttcaa acccataaac gcaacattta cacttatgga ggcgttgaaa
    19681 atgctgatgg tacaaatacc tcaaatggcg gttggtaata ataactttga aaaattaaaa
    19741 caatgaaact cccatattcc atctgctctc atcaaaaaac atataatgat ggcttaaata
    19801 gctaattttt aaccaattaa aattttatac agatgaatat aaaaacatat atcaatattg
    19861 taagtatagg aatcatcgca tcattggttt catcttgtgg caatgatgat tacgactgga
    19921 acaatgtcat tcccggaagc caaagaatta ctactttgga taatgatact gttaaagtta
    19981 aaattgacac tattgaaggc aataatactg atgttgtaac attgaaggca attgcccacg
    20041 gaggctcaac ctttgactgg attgcaagct caaacgtttt aataccccaa aatgatgctg
    20101 agaagaatta cattgttcat gttaaagcca attctaccca agacacttat gcttggctaa
    20161 aagtacagga aaccacttat ggaggcataa agggcaaacc agattcaatg cagtttttca
    20221 ttattggcta ttgcgaattc aacaagcaac aactattagg aaatggttct tttgtaagta
    20281 aaatgagcta ttatgcccca tttgatgtta atctatccga aggtaagggt gacaccattc
    20341 tttacaacaa cttcaacaat atgcgttggc aactgaaatt agtcatcagc aaagatgttt
    20401 atcagaaaat cactattccg gccaaccagt attttgtata taatgatgaa atagtaactg
    20461 taaaaggaaa cggcaaatac aacacctgta aaagtttgtt tgtgataaac tatgcaatat
    20521 gccgtttatc aggcgacacg ctggcttatt cgtccggagt tgattcgctg aggataaaat
    20581 aagaactaaa ctttcatccc caacaccagt acatcatcca tttgctcaag gtttagcgac
    20641 cattgcaaaa atttttcttc cagaaaatag cgttgagctt ttaccggcat gacagaagct
    20701 tcgagcaata actggtaaaa gccctcgcgg cctaattttt tggaacgctc gcccccaaac
    20761 tggtcggcat atccatcaga aaacatgtac atcatatccc cttcctgtaa ctcaaattcc
    20821 tgctgcgaaa atgattcttt ttcggtagga taaatgccaa taggcatttt gtctggcaga
    20881 aggctattta tctttccatc gcggacaacc agcaacggat tgttagcgcc cgaatactga
    20941 atttttgttt tattccggtt aataatgaca agggctatat ccatgccatc ttttgccccg
    21001 gtcttgtcat cctggttgag caaagttttt accttgatac gcaattggtt aaggatgtcc
    21061 ttcgcttcaa tatctttgtt tttgctgaca atctcattca ggtaagcaaa gcccagcata
    21121 ctcataaaag cacctggtac tccatgtcca gtacaatcag caacagcaat aactgtgcgt
    21181 ccatcatgaa ccgagaccca ataaaaatcg ccgctgacaa tatctttggg cgaaaaatat
    21241 atgaaatact ctggtaaata ggtattcagc acctcctcgt gcggcaaaat agcggcttga
    21301 atgcgacttg cataatttat actatctgta atctgttttt tctgtatatt caggataata
    21361 tatgctttct tcttttgata atactctata aaaactaata tgagcaaggc aagaacaata
    21421 cataaaccag ctgcaagaat ccttgtaata agcttctggt gctcaatttt atcatgctga
    21481 gatttcaaca tcaaatcttt cttctccgtt tcatacaaag tttgcatttc ggcaatcttc
    21541 tcggcacttt gagaattgta aagcgaatct ttgtaaaggc ttgctttttc actacatttc
    21601 caagcctcct tatataaacc tttatctgaa taaatatcgc tcaaaaccaa atagcagtcc
    21661 ctgatatctt cttttgaatc aaccaactta gccaattcta aagcctgatt ggcatatctg
    21721 gcagcttctt cccggcggct atgcttatta tatattccgg ccatttttgt aagcgatttg
    21781 gttaacgaag ccttatcatc cagcattgaa tctattcgat aagatttttg gtaatacacc
    21841 aaagctgagt catattggcc tgtctcataa tgaatttcgc caatattaaa cagtggcata
    21901 gacattgccc tgatcatgtt atttttttca tatatagcca gagccttctt aaaatattcc
    21961 atagcagccc ctgtcctacc cttgaaataa taaacaatac caatattgtt ataggctaaa
    22021 gccattcctt tgatatcttt tattgactca cgcagtttta gcgacttatt gtaataaaat
    22081 aaagccaaat cgtagttttt ctggtagtaa aacaccaagc caatattcaa caccacacgg
    22141 gcttcattat acacatcttt tgcctgttca aaccttcgga gtgcattgaa caaatttttc
    22201 agggcaggct catacttgcc cacatcaagc atggttaccc ccaggttatt ataagctttg
    22261 gctataagtg ttgtttttct tgaagaattg aaaaaatcaa gggctttatt aaatgactca
    22321 atggcagctt gtgtattgcc tctcttttta taatactcgc cttgcattaa aaaagacttg
    22381 ccttttagcg aatcagcatt caatttttcg cctatttcac gatacttcgc caactcagag
    22441 aaaaataaag cagaatcagc atctgccaaa tcggaaactt taaccccaaa agcatataaa
    22501 ccagttgtat cagaatttgc agctataata ggagccttga aaaaactaac agttacaaat
    22561 agtaatgtta aaagaatttt aacaagcttc atattaaaaa aattaaagta aaaatttaac
    22621 tttgagaaat attaatcatt aatacttctt cacggtttac accaatgaga ggcgcttagc
    22681 aaatctcatt ggttttttta ctttaaatcg gcccctaagg tttcattaaa actgcccatt
    22741 ctgtaaccta acatatcaac agtgacaaaa tcgaacccaa acgagcgtaa agcagcacta
    22801 acctcgctgg ccaatggttc tgacatgatt tgtacgcgtt ggtcagcagc cacttcaata
    22861 cgggcaagtt tgtcgtggca acgcacacgc actgtcctga agcctttatc aataaggtac
    22921 ttttcagatt tttcaatctt taccaaatct tcttctttga tagccgttcc ataagggata
    22981 cgcgaaaaca ggcaggcata agctggtttg tcccatgtag gcaaacccat cattttcgaa
    23041 tactttctga tttcatcctt tgtaaaacca tgcgttttta gggggctatg cacttgcaac
    23101 tcatcaatag ctttagctcc gggcctgaaa tcattgacat catcggcatt actgccatcc
    23161 agaacatatt taatcccatt ctttgcagct tcctcaataa cattgccaaa ttctattttt
    23221 ttgcaaaaat agcagcggtc aacagtgttc tgcctcacct cgtcttctat ttcatttgac
    23281 tcaacaatga taagcttaac cccaattatc tgcgccaaat gcctggcatc ttccagctca
    23341 tatcgaggaa gcgcaggaga atcaatggtg atgccaatcg catcgtctcc aactgcctgt
    23401 cgggccacag ccagtaaaaa ggtactgtca acacctccag aaaatgcaat tgctaccttt
    23461 tctaatttct tgatataagc tgttaagctt ttaaattttt cgtccatctg tgctgttttg
    23521 aaaactgaaa acaatgataa aaccattatg ttcagaagaa ttaaaaattt caatctgaca
    23581 ggttgtaaag taagcttaat tttattggag aaatattaaa ataaatctaa aaacaatcat
    23641 tgtccggtac aaacaggtcg ccgctatgcg gcttagaaac atttggtgta tttggttatc
    23701 ctgcaaacag ttcgcagcta cgctgctatt atttagtcgc gtagcgacgt cctgtttgta
    23761 gtaaaattaa acatcgttaa aattaagccc cggcagggcg accagtttct aatcaacaat
    23821 attacttgta tttataagta tctattttat tttaccgaaa aaaagtgaaa aacaatagca
    23881 ttttgggtta aactatttaa cctgtaactt gttaaacaag tattacaata agtatatcaa
    23941 aaaggaataa ggatattagc taaaaaatat attaaccttt aacattgata cttatgaaaa
    24001 acctaaaagc tacatacatg ggtttggaat tgaaaaaccc aatcattgcc ggttcaagca
    24061 acctaagctt tgatatcgat tctctgaaag gacttgaaga tgcaggggct tctgctattg
    24121 ttttcaaatc gttgtttgaa gaacaaatat tgttggaaaa agccattctt gaagacgacc
    24181 ttgatgaata tgccgaacgc catgccgaaa tgaccagctt atttcctaaa cttgaacatg
    24241 ccggggcgaa agaatatctt ttgaaacttg aaaaagcccg caaagttgta tctgtcccct
    24301 tgtttgccag cctcaatgcc attttcaaag aaacatgggt tgaatatgcc aggcaaatag
    24361 aacagaccgg ggtggatggc attgaactta acttttttgc catacctaaa gatgctgata
    24421 ttaccggctc agatattgaa aaacaacaat tggccattgt ccacgaaata aagaaaaaca
    24481 tcaacatccc gataagtgtg aaattgagcc cgttttattc caatacgctc aatttcataa
    24541 aatcattgga caaagaaggc gttgacgggc ttgttttgtt taacaggttt tataaccccg
    24601 acattgatat tgaaaatatg aagtttgact cattgcacca tctgagccac aatgatgaaa
    24661 gcaagttgcc attgcgttat gcaggtcttt tatattctaa cattgcagcc agcatttgct
    24721 gtaatagcgg catacatcaa ggtacagatg taataaaaat gcttttggcc ggtgcagatt
    24781 gtgttcaggt agtaagcacg ttgtaccgca ataaattgac acatataaaa accatgcttc
    24841 acgacattga gttatggatg gatttccaga atatcaaatc gctggaagaa ataaggggaa
    24901 agctatcccg caaaaacctt cacgacccct ttgtgtatca acgggcccaa tacattgatt
    24961 tgctcatcaa atctgacgaa ttgatgaata aatatacatt acggtaatca tttcattttt
    25021 tttacaaaaa aagcagacct gttgaaaagt ctgctttttt aattagtaaa aactaccaaa
    25081 tttctatttc tttgtagttt tagaagtttt ctttttgccg cctgcacctg gttctggtgg
    25141 gtctgggaga aatacaacag tgtttttcat aatataaatt ttttaaatga acaaacttat
    25201 taatatacaa gcatttagaa tactctttgt gctgttatta ataatattaa taatttcgtg
    25261 taagataaat aataatcctc aaaattacaa agaaattttt caaaaatctg agacctattt
    25321 ttctaataat gaattcttta acgccagtcg aggttatcaa tcttatatca actgcccgca
    25381 aattgatgaa tactctttgt ttataagcta tggaaaatta tttatctgtc tgcaccgcat
    25441 gaaagataaa aatgcccgcc aggcctatga cagtgcttta gcacatatcc ccaagctaaa
    25501 agacaaggct gttgaagatt tgctttataa gataaaaagc attgatttgc agaaaaatga
    25561 agagtattta aaggctattg atttgacccg caaagaaatc tgcaaataca agcttgacga
    25621 tactttgagt attgagcatt ttcataactt gggcggcctt tatcaaagca ttggcaacaa
    25681 agacagtgct ttatattatt acatcaaagc caaacagctt ttagacaaga aaaaagacaa
    25741 gttcaaccaa ttgtctgcag atatttacaa tacctatgcc tattgccagc gttattatct
    25801 gaacacccca ttaaatgctg caaaatatta cgacagtttg ttgtatgtct ctgaaaaaat
    25861 actgcataaa gattcagatt attatgcatg gaacctattt aattgcggca tattttacca
    25921 ggttattgga gctaacaaca aagccctgca taattattac aaagcttttg aaacatacca
    25981 aaaaataaat ggcaactttg atgaagaatg taactacatt caggttttta tcgccagcat
    26041 tgaattacac ttagggaaca tccagaaagc atctgataag attaataatt ccgatcttta
    26101 ttacaaatca accaaagatt attcgaatat tgctaaagca tacatttttg atgcctactt
    26161 aaaaactaaa ctcggtaaat accgcgaagc tttgaatatt tacgataaat atatcaggct
    26221 tgctgattcc cttaaattat ctacgaaaga aacgcactat ctcgatattg ccacgtgcta
    26281 tgtaaagatc aaccaaaaag actcagccaa ctattggttt gagcaatgct tcaacaccct
    26341 gaaaagtcct aaaaagaact acctgattat gttacatact tttttatggg agtatgttgg
    26401 tttttgtttg gctaatgacc atatagccaa agcatctgaa ataatcaggt tttatgaaag
    26461 taacctactc aaaagctatg gtaacaacag ccccaactat gcccttatgc tgttatgcaa
    26521 agcccgtatt gctgattatc agaaagaata cgcaaaaggc acagattttt acctgcaagc
    26581 attgaaaata ttggaaaaca aatcatacgc caattctcaa accaacaaaa acatctcgct
    26641 tcagcgatac aacaatttgt tggctaaaga aatcattgac tgtacaaccg ggttagccag
    26701 agatttcaaa catcttgccc aaaaatcaac agacaaaatc aaattcttac aaaaaagttt
    26761 tgaatactat aaacaaacgc ttgaattgat ggggcaaatg aacggtaata tccctgtaga
    26821 atgggacaaa ctgaggtttt ttgagcagtt caatgcagta aaaaaagaga tacttgaagt
    26881 caaaattgcc caataccgaa acgaacccca agtcggtaaa aaaaaccaat tgcttttgga
    26941 ggcttttgaa atagccgatg ccaacaagct tagcatcatg gtcgaaaagc ttaaagaaaa
    27001 gcagtttaag cagcaattag ggatacatga ttctatactg aaaaaagatg agaaactgtc
    27061 cgaaaacatt agctttttgc aacatatcat tgatgaagaa gaaaagcagg agcatcccga
    27121 caatcaaatg ttggagaaat attacaagct cattttcaaa acatataatg aatacgatag
    27181 tttaagagag tttataaaca agttatatcc ggctattcag gatatttatg gagccaatca
    27241 tcttagaacc agctataaac agatgattca ttgcttgcct cagaatgaat tgcttattga
    27301 atatgctttt acggctgaca gcttatatgc ctttgctgtt aaaaatggct ctatttcatt
    27361 attttcagta ccttctgaaa taattacaag taacattcaa gattttaggg cacttttgac
    27421 aaacgtaact gaccaagcct atacacatca aggtattaca acatacacaa acacagctta
    27481 tagtatttac aagcaggctg tttttcctgc aatacaaaat gccagttttg aaaggcttac
    27541 catcattcct gacggcatgc tgacctttat tccctttgag gcattggtta cccatagcgc
    27601 aacacgacaa aacgctgatt atggtaccct tccctatttg tgcctttcag ccaatatcaa
    27661 ttacacctat tcattggctt tatactctgg ccaattgagc cattattcca atgtagcatc
    27721 aggaattacc ggctatgccc catcttatga caaaagcagc cctttaagcc ctttgcgaaa
    27781 tgccgaacta cgcaaaaagc tgaaaaacct caaaggagca agcgatgaag tttatagcct
    27841 tgttaatgca tacggcggac gaaagctgat tggtaaaacg gctaccgaaa ctgcttttaa
    27901 acaagcccat aacagcaaac atatactgca cctggccatg catactgtca ttgatgatga
    27961 aaacccaatg tattcaaagc ttgcttttgc agaggaaaaa gattcggtca atgagggctt
    28021 tctgaacact tacgaaatat atcccctgaa aatcaattcg ccactggttg tgctcagtgc
    28081 ctgtaacact ggctacggca aaatcataga tggcgaaggg attgtaaacc ttacccgtgg
    28141 tttttactat gcaggctgca attcgctttt aataactctt tggaatatct cggaccaaag
    28201 tagtgtccaa ctcatgaagc ttttttataa caacctcgat gcccatgatg ataaaaacgc
    28261 agctttggcc aaagctaaag tcagctacat agcaaattca gactcaagat attcccatcc
    28321 ttattattgg gcaggattta tccattcagg aaacgcagca ccattggcta tatcaaaaat
    28381 cgaaacatcg gcatacatta tactttcagt gcttctattg ataactattg gattatttaa
    28441 cacaaaatgg aagttcaaat aacattaata ttttaacatt gcagaagaat tcatttcatg
    28501 cttaaaaagg ttttcatatc tgcttttatt tttgcggttt caggcattgg gatacacgct
    28561 cagtgcaatg ttgccgaaag tgtttttcaa ccagaggaaa aatatgatta tttggtttcg
    28621 taccacctta gttctatttg ggtaaaagct ggtgtagttt ctttcgttac ccacaaaacc
    28681 acatacaggg gcaaagattc atggttttta gagagctatg gaagttcatt gccccgtttt
    28741 gattggtttt tcagggttcg tgatacattt agggcatata ctgacgctga cacatttgtc
    28801 cccgacttcg ccgaaagggt gtctgatgaa ggcgggtata tagttatcga gcaatacaat
    28861 tacgataaaa tccgaagtaa gatattctcg agggtatatt cgagcggaaa gccatggaaa
    28921 tatgacacat tcaaatatga accttgtatc aacgatgtat tgacagccgt ttatgctgcc
    28981 cgtaatcaag atttcagcaa tattaagccc caggaaaaag taccatttca aacccttgtt
    29041 tttggtaaga tacaaccgct atatattcgt tttttaggta tagaaactat acaactcaaa
    29101 aacgggcaaa aatttaactg tctcaaattt tcgtccaaac tggttgaagg taccatattc
    29161 aaaggcggcg aagatatgtt tgtttgggtt accaacgaca aaaaccatat tgctgtaaag
    29221 attcaggcaa aaattgtcat cggctcggta gttgccgaat tgaccagctt caaaaacaac
    29281 aaatatccac taacctcgtt gcagaaataa tacctatctg ctgattattc tttctatctt
    29341 tgcggcctcg atgattttga ttattttgcc atgcagaaac aaataccaat agccgaatac
    29401 acttacgatt tgccagatga gagaattgcc aaatacccac tctcccatcg cgatttgtct
    29461 aaacttttgg tttacgacaa aggcaatatc ctttcggaaa aatttggcag catatcgcag
    29521 taccttccgg aaaacagcct tttggttttc aacaatacca aggttatcaa agcaaggatg
    29581 atgtttcaga aggcaactgg tgcagctatt gaaatcttct gcctcgaacc tgaaaatcca
    29641 tcggattatc agcaagcatt caaccaaaca agacgttgct catggaaatg cttagttggt
    29701 aacctaaaga aatggaaaag cggcgaaata accgctattg tcaacattaa taataagcca
    29761 gtcaccatca aagccttgct gttgcagaat atgcagcaat ggcagatagt gcaattcaat
    29821 tgggatgctg aaaatataac ctttggacag ttgctcgaaa atgcaggtca gattcccatt
    29881 cccccttatc tcaaccgcga aagcgagaat atagatttac tgaggtatca gaccatctat
    29941 tcgcaacacg aaggctcggt agcagcccct actgccgggt tacattttac tgatgatgta
    30001 ttcaaagctc tttcatcaaa aggtgttgaa caatgtgccg tcacccttca tgttggagct
    30061 ggcaccttta tgccagtcaa gactgacgat gcattgcaac acgatatgca tgccgaacgt
    30121 ttttatattc ctcttcaaaa cattgaaaaa cttttaaaaa acataggaaa cataactgcc
    30181 gtgggaacta cttcaatgcg ggcattggaa agcctttatc aaattggagt tgcgttaatc
    30241 ttaaaaaaga ataacccttt tttggtgagc cagtggcaat acagacaagt aaatgaacaa
    30301 attacggctc ttcaagcact tgaggccatt ataaactatt gcaaagaaaa gaatttaaaa
    30361 ggcattgaag ccgaaacacg gataatgatt gtccctggct ataaattccg catggtaaac
    30421 aggctaatca caaattttca tcaacctcaa agtacattgt tgttgttagt agccgccttt
    30481 attggccccg attggcgaaa aatatatgat tacgcattag caaacaatta tcgcttctta
    30541 agttacggag acagctcttt gctgatacct taaaaagctg tatattgaaa tgacaaacgc
    30601 tttactttta ataaaagcaa agcgttttac tattttaaac tgagttctaa taagcaatca
    30661 cgccagaacc aacaagttcg tcttctgcat accaggctgc aaactgcccg ggagtaacac
    30721 ctcgttgggc ttggtcaaaa acaatagcca agccattttc caacttcagt acagaagcat
    30781 cctgcaacgg ttgcctgtaa cggatgcgaa ccctgaagcg ctgttcatcg ccttcgtcca
    30841 aagccaaatc aggccttatc caatgaatat catgctctga tacatacaaa gctttgcggt
    30901 acaagcctgg atggtcttct ccctgcccaa catatacaat attattaaca acatcaatgg
    30961 ctataacaaa caaaggtttt tgcataccac ctatattcaa ccctttacgt tgtccaatag
    31021 tataaaaatg ggctccacta tgctttccaa ccaccttacc catatctatt gtataggaat
    31081 aaggttgcat agtatcatat aaatcaagat tgttcttttc tttataaaca gggcaatctg
    31141 aaggtatttc caccaccaca ccttcaacag ctttcaactt ttgttgaagg aatactggca
    31201 agtctatttt tccgacaaaa caaacccctt gagaatcctt acgttgagct gttgccaacc
    31261 caagagactc agctatctgc ctcacttctg gtttggttat gtggcctata gggaacatag
    31321 ctgttgacaa ttgcttttgc gaaagttggc acaaaaaata gctttggtct ttatttccat
    31381 caacaccagc caaaagcctg tatttctggc cattttcagt ttcgatagcc tcacaacgtg
    31441 cataatgtcc ggtggctaca tattcggctc catatttctt agctgcatca acaaaaagat
    31501 cgaacttaat ctccctattg cacaatacat ctggatttgg ggtacgccct ttttcgtact
    31561 cgctaaacat ataatcgacc acacgttgtt tgtaaaaaac gctcaaatca accgtgtgta
    31621 agggaatatc aagtttccgg gcaaccaact cggcaaaggt aaggtcgtct ttccaggcac
    31681 aatcgccata aagagtacct gttgtatcat gccagttgat cataaacaaa ccgataacct
    31741 cgtagccatc ttgttttaac aaccatgcag caacactcga atcaaccccg ccggaaagac
    31801 ctataacaac tttttgggcc atttttattc attttcagga gcaataggat cgggctgaat
    31861 atgtgcccaa ttttcaccac gcttgatgcg ggtaatctgc atctcgctaa ctttgaacat
    31921 tttagcaatg atatgttgtg gtgttttacg caaaagcata gattttataa gcttcacgtc
    31981 tttttcgtta agcttagagt aagttatcag ttcttctttg ctttttttct gtgtcttttc
    32041 tttcatcagt tcagacacac gtttcaggga ttcttccctg gtaatccatt gcaaattctt
    32101 ataatagtta ttgcgaatat taccatctat atgcattaca tgaatatgct tgtcagaagg
    32161 tttttcgagc caaacccatg caaccaattt atgcacatac ttgctgtaag tcaccttttg
    32221 caggctaaca gcaaggtaat tcttcatctt ataaaactta acaatctggc cttctttttt
    32281 aaaatgaaaa gatttaatcc tgccataatt acttataaga tatttacctt ccgtacccgg
    32341 aatttctttc cattgctcat cattaagttt cctaagccgt ctttgctctc cctccatagt
    32401 ccttgatttt taagttaaaa aataataacc atcaaagtaa attaaaaatc tacttctaca
    32461 aaatcctttt tagacacccc acaaaccgga caactccacg tttcaggtat atcttcaaag
    32521 cttgtgcccg gggctatacc gctatccggg tctccttcct gcgggtcata aacatatcca
    32581 caaatgatac acctgtattt tttcatttct ttgataaata aatctttgca aagatataat
    32641 tacttataac atggtataaa cgttataagt aaaaaatagt taaatatctg aaaatacttt
    32701 atatgacgtt aaacccaagc tttttgagga tatctttttg aattgttttg ccatgaataa
    32761 gttttactgt ataagcatta aactcccttc ggacagcata attttcacga taggactcaa
    32821 aatcctgagg atgagatttt aacatatttg aatcgctgct aataggataa gtatgcaaga
    32881 tggcgttaca aataacctgc tgatcgccca tattagagca atcaatttct atcagcggct
    32941 gctgaggctg aggtatatca atttttattt tgtcaatccc caaacgaaaa aaattattca
    33001 acgcctttac agacataaat gtagcattgg ctttgccatc tgctgaatat ccggctatat
    33061 gggatgttcc aatggcagat ttgtcaagca aaccaacgtt caacgcaggc tcattttccc
    33121 acacatcaac cacagcggta tcaatccttc cattttgaag gctattcaaa agagccaaat
    33181 tatcaacaac ttcaccgcgt gaggtattga tgataatggt tcctttgttc accttttgca
    33241 tttttttctc gtccaaaagg tgaaaagttt taaactctcc gctataagtc aatggtgtat
    33301 gaaaagaaat gatatccgat tcgcgcaaaa tcccatcaaa tgaaatataa ccacaaagat
    33361 ttttggcttt taccaatggc gggtcattca aaaaaacacg catcccaagg gtttcggcca
    33421 gcctgaccac ccttgttcca acatgcccca ctcctacaac ccctaagctt ttttctacaa
    33481 aataaaaacc cttttcagtt gctaaatgag caaaagcagc tgccatatat tgcattaccg
    33541 aacctgcatt gcaccccggg caatttgtcc aaacaatacc atttttttca caatattcag
    33601 tatcaatatg atcaaaacca atagtagctg ttacaataaa tttgaccttg ctgccattga
    33661 gcaaatcggc attgcacaga gtacgggtac gtataatcaa cgcatcagca tctttcacca
    33721 attcttttgt tatgtctttt gcagcaaggt attcaatatt gcaataaggc tcaaggatac
    33781 ctttcagaaa tgggattttt tggtcggcta taactctcat tttagtttat tttgttacaa
    33841 taataaaaat ttaatccata ttgttttgta aaatattctt aaaatacttt aaaaccttat
    33901 tattgacaat attataaagg tcaaaaaaaa catataacaa caaaactttt ttctttttta
    33961 tgaatttaga attttatccc aacttatcaa agtatatttt ctaaaaattt ttatttaacc
    34021 caatattatc ttacatggca caatggaacc atggcatggt tgccataaat ttgtcaaaga
    34081 tggacgtttg tacaatatca aaaggcaatt ccaattatta gcaagccaga aaggcaggga
    34141 ttaattatcg gcgagtgtaa tattataaag tataaaaaaa gcgaaacagc catggaagtt
    34201 ggctgtttcg caaaatagaa gtgcttataa ataaaaatac taggaaatac ttaattaacc
    34261 tttgtacttc cggaacaatg atacaaaaac gtgtcctcca aagccaaagg tgttgtttat
    34321 ggctacgttg acttcttttt tcactgcctt attgggtgta acgttcaacc ttttgtcaat
    34381 agcagggtct atttcgttta cattgattgt tggaggtaca atgcctgttt ctattgcttt
    34441 cacgcaagca atagcttcaa tagctcccgc tgcacctaat aagtgacctg tcattgactt
    34501 ggtagcacta acatgcagtt tgtcaagcga tttttcaaac actttacata gagcatgaca
    34561 ttcgctcagg tcaccaacag gggttgatgt tccatgagta cttacatagt ccacatctgc
    34621 gggggttaaa ccagcctcat taatggcatc aagcattgca gctacagctc cttctccatc
    34681 agggtgcgga gcagcaatat ggtatgcatc cgagctaaac cctgagcctg ttatttcaca
    34741 gtatatctta gctccacgtt ttttagcatg ttcaagttct tcaaggagaa gtaccccact
    34801 accttcgccc ataacaaagc catcgcggct tacatcaaat ggacgtgaag ctgtttgagg
    34861 cgaatcgtta cgttcagaca atgctttcat tgagctaaaa ccaccaacag cagccttagt
    34921 aatacaagct tctgaacctc cggtgaaaat tacatcagct tttcccagtt tgataaaatt
    34981 aacagcatcg ataatggcac tattgcttga agcacaagct gatgtggtaa catagctgat
    35041 accttttaat ccataacgaa ttgaaataag gcccggagcc atatttgaaa tcatttttgt
    35101 aataaaaaac gggctaaaac gaggcatttc agggtttttg ctaaatgaaa ccacctcatt
    35161 ctcgaaagtc tcaatacctc caatgcctgt accccatata attccagctt tttgttgatt
    35221 aatcttagta acatcaagcc ctgcatcctt tatacactct tctacagaag caatagcata
    35281 ctgagaaaac agatctgttt ttctaacttc cgttttttct ataaacatta aagggtcaaa
    35341 accttttact tctccagcaa tacgagtttt aaaagcactt gcatcaaacc tggtaatatt
    35401 tgctatgccg ctttttccct gaactaatgc atcccaaaag gaactaacat tgttcccaag
    35461 tggggtaaca gcccctaatc cggtgactac tacacgtttc atattctttt attttaatta
    35521 atgaaataat cttgagacaa taataaatat aatctattat tactaaaaaa actaaacttg
    35581 ataaggttta ctttgcatta taggcccatt tcaaataaac agccccccag gtaaatcctc
    35641 ctccaaaagc tgataagata aggttgtccc ctttttttaa ttgtctttca agctcccata
    35701 aacataacgg aatagttgct gcagtagtat ttccatatct ttcaatattt accattactt
    35761 tttctttggg caagcccatg cggttaccta cagcatcaat aatcctgagg ttagcctggt
    35821 gtgggataag ccaggctata tcatctggtc tcaggttgtg tttttccatc atagctacag
    35881 aaacatctgc cattttcgaa acagctactt taaaaacagc ttttccatct tggtatatat
    35941 aatgcatttt agcatcaaca gtttcgtgag aggcaggcct taaggagccc ccggccttga
    36001 ggtttagatg gtctttaccc gaaccatcga tatgtaacat tgcgtccata acaccaactt
    36061 cctgggttgt aggttcaata agcattgcta cagcagcatc accaaatagt acacatgtag
    36121 tacggtcggt ataatcagta atacttgaca ttttatcggc tgttaccaaa agaatttttt
    36181 tgtatttgcc tgattcaatg aaagatgatg ctgtttgcaa agtaaataaa aagcttgaac
    36241 aagctgcact aacatcatat ccccaggcat ttttgatacc cgcataatca gctactaagc
    36301 aagctgttga agggaatgcc atatctggag taacagtcgc aacaatcacc atgtctattg
    36361 tatcaggtga ggtattggtc tttgccaata attttttggc agcttcgccg cccataaaag
    36421 ctgttgcttt gtctttgtct ttaaggatac ggcgctcctt aataccgaca cgctgcatta
    36481 tccactcatc agaggtgtca accattttac taagctcttc gttggtcaat acatattcgg
    36541 gcaaataagc ttcaataccc gtaatggccg ctttaatctt gttcattgta aatgtttttt
    36601 aagaaatata ttactatatt aaatgttaat tccgcaataa ctaatgtatt tataaagtca
    36661 aaagccagct ttgggccggc tttgtcttgt tttgtttagt tacatgcttg gcgtatttgt
    36721 acccttcgta tttttccact gattgtcttc ggcaattcgt gcacaaattc aactatgcga
    36781 gggtatttat atggagcagt aacttttttc acatgttcct gaagttcttt ggccaaggcc
    36841 tcgcaatgtt caaagccttt tgctgtaaca atagtagctt tcactatctg tcccctgatt
    36901 ttgtcaggta cagccgtaat tgcacattca agcacagcag ggtgttccat cagggcgctt
    36961 tctacttcaa aaggcccaat acgataccct gagctcttta ttaaatcatc agcacggcct
    37021 acaaaccagt aataaccatc ttcatcgcgc catgccatat cgccggtata atatacatca
    37081 tcgctccagg ccttttctgt cagttctttg tctcgatagt aacctccaaa catgccaact
    37141 ggtttgcttt taccagtgta aattactatt tgtccgggtt ctccaatgcc acaagagatg
    37201 ccattgtcat taacaatgtc tatatcataa ccaggtgatg gacgtcccat cgagcctggt
    37261 tttggctcca tccaggaata ggttgctaaa gctaccgtac attcagtctg accataccct
    37321 tccttaagtt taagcccaac agcatcataa aactgcttaa atacttcagg gttcaaaggt
    37381 tcgcctgcta caacacagta tttaaggctg cttaagttaa attttgaaag gtcttcctta
    37441 attaaaaagc gatatatagt aggaggtgca caaaatgtcg ttacgttgta tttttggatg
    37501 atttcaagca gttttaatgg tacaaactta tcataatcgt atgtcataac agcacttcct
    37561 gaaagccatt gtccatatag ctttccccag gcagattttg cccaaccagt gtcagctact
    37621 gtaaaatgta aaccattttc ctgaacattc tgccaatatt tggctgtcaa tatatgtcca
    37681 agcggatatg tgaaattatg gaaaaccatt ttgggcatac ctgttgtgcc agaggtaaaa
    37741 tacaatagca ttacatcatc attacatgta gcctcacttc ctgtaggtcg tacaaaaatt
    37801 gatgatgccc tttccaattc agttgtgaaa ttgaaccaac cgtctttatc ttctccaatg
    37861 attgctttgc atttcaatgt tggagattcg tctaatgcag cttctatttg atgaacaatc
    37921 tcactatccg aaacactgac aatcatcttt atgccagctg cgttatttct ataaacaatg
    37981 tctttctttg tcaataaatg agtagcagga attgcaatag ccccaatttt atgcaaacca
    38041 agaatactaa accaaaattc gtaacgcctt tttaaaatga gcattacagc atcgcccttt
    38101 tttattcctg cagaaagaaa aaaattagcc gctttatcac tgtaatattt catctggcta
    38161 aaggtaaatg tagcttcagc accattatca tcgcaccaaa ccatagcaat tttatcaggc
    38221 tgctcattgg caatttcatc aacggtgtca aatgcaaaat tgtaattttc aggaactttt
    38281 atttcaaagt tctttttaaa gtcatcataa gataaaaaat ctgtttttac aagatatttg
    38341 tttaacatct ttttctaaaa ttaattaata aatagccaag cccgagccta gaataatgag
    38401 caaaggaact aaagtaatta ttcagaggct aataaaaggg ttatgcaggg taaaacgtgt
    38461 ggctaaaatt cgtattttgg ggtgaaattt tatatttagg gttgaatttt atttaaaata
    38521 atcaacctga tttattttaa actgggctta ataaagctat tttcgaaaca aaaaagggtg
    38581 tagtatattt aaatttatgg aatattgcaa ttcaatgtcg tttagtcaag gctctaaaca
    38641 aatgtaaatt tgacttgact atttgtacaa ccaggcacaa tattataaac cataacgcct
    38701 caaataatga agaaaattgt tttctcatct aaattagctt ttaaacaata aaagcgaata
    38761 aacaaaaaaa cctttttgta ttggtcatca agagttaaat tatttttatc agtcatttgt
    38821 taaaattaaa aaaccatggt actcttcgtc ggcaacgata ttaataacat ccaaagtaac
    38881 tccaacaaca gttgggaaaa gctattaaaa agaattatcg agaagtatgg caaaaaagtt
    38941 gaagtggata aatcccaacc ttttcctttg atttatgaag agttgtattt gaaacttttg
    39001 aaaactggca aattggaaaa tgaattaaca ctaaaagaag agatagccaa agccctatct
    39061 actattaaac caaacgaaat acatagaatg ttgttggagc tggattgcaa taattatgtt
    39121 accacaaact acgattatac ccttcagcag gctgttttaa atgggaaaac aacaaaaaaa
    39181 cttaaaaatg aaggactggt atatgaaaag aaattcagcg tttttcgtca tcatgttatt
    39241 aataataaaa aattttggca cattcatgga gaaataaatt caccacaaag catcaacctg
    39301 ggctttgaac attatggcgg gcagctgcaa catttgcgcg attatgttgt atcaggcgta
    39361 aagtataaaa caagagaaaa cccaatagct cctttatacc aacgattaaa agaaaatgat
    39421 gtaaaaaaca tttcatgggt tgacttattc tataccgatg aaattcatat tgttggtttg
    39481 ggcttaggtt ttgaagaaac agacttgtgg tggctgataa ccaaccgtgc caggcttaaa
    39541 tatgagaaaa agctggatgc cctaaaaaac aataagatta tatattattg ccccttgtta
    39601 tataaaaatt atcacaaaaa acaaataatg gatgctaatg gcattaaaac aatttatatt
    39661 aacaaaataa acactgaatt ttattttgag gtaataaacc gcattaaaaa caaaaacact
    39721 attgcctaaa cctacattaa acaaactcta acttactgac ggactatagt tctgatactt
    39781 ataccggaca acagtgaaaa atatctggat tttacacgaa acaacaaaaa tcgtccataa
    39841 aaatcttgtc gaagtttcat tttcgtctta ccttgctttt gaaatattaa cattttttta
    39901 aaacttcata aaaactacat aaacacaact ttaactttaa ctaaaaaaca cttaaacatg
    39961 gaaagtgatg ttgacaaaaa gcaagatttc tctattaaag aaagtgaaga aatttattca
    40021 aaagcagtaa gggcaggaaa aagaacttat ttttttgatg taaaggccac aaaatttaac
    40081 gaattatacc taactgttac agaaagcaaa cgaaagcaaa accaagacgg ttcgttctat
    40141 tttgaaaaac acaaaatttt tctttataaa gaagattttg aaaaatttct tgacgggata
    40201 aacgaggcca ttgactacat caaaactgca aatccaatca ttataaaacc tgccgaaaac
    40261 caacttgatt atccagaaga acaatttgta tctattgact ttgaagacct tgacacaaaa
    40321 atgtaataaa aaagccatca aattgatggc tttttttatg attattcatt tcttacttat
    40381 ttattgttcc acaaattaga aggataaatt cctttgccta tcaaatctct caatctgttt
    40441 atggtcatat ctttatcttc tttgtaagta attccaaacc agttgtctgg cgaacgaagc
    40501 acttttactt ttgcattgcc agcttttaca atgttatcaa cggccatagg caagtaaaac
    40561 tcagcagtag cactatcctt attagtcttc atgaaatctc caaactgttt ttcaagatgg
    40621 ttaaatatac ttaccgggaa cccccagaaa ttcatcgata caacctcatt ctcagccaaa
    40681 ggaatcattt tatcttcaga ctctacaaaa acaatcttgc catcttttct ttgaattttt
    40741 gtccgctcaa caattccttc aagctggctc tgtgaattta ctgtacatac acctcttgat
    40801 acatgcccat gctctgaaag cgtcctggat agctcaaaac caaccatgct ataagtattc
    40861 ttatcatcgt tggctgtttt tataaggtgt tctgcaataa gtttgtatga ctgagcccca
    40921 taaaaatcat ccgcattaat aactgcaaat ggggtggtaa ttttagaagc tgccactaaa
    40981 acagcatgac ctgtacccca aggttttttt ctgtctgcag gagaggcata gcctgaaggt
    41041 aaagcatcta actcctgaaa tgcatattca acttcaatgt gcttttcaaa cctttgaaaa
    41101 acttctttaa aatcagcttc aatacttttg cggataacaa ataccacttt cccaaaacca
    41161 gccctgatag catcataaat tgaataatct gtaattgttt cgcctgaagg gccaatttta
    41221 tcaacctgtt ttaaacttcc ataacgactg cccataccag ccgctaatac cagcaatgtt
    41281 ggtttaatct ctcccataag ttttttgtgc aaaattacat ttatttacga caattgaaga
    41341 atattcaata ccaatgtgtc attcattgcc ttcaatctca ataaattcaa aaggataatc
    41401 aagcaaagtt gaataatact cgcctctttc cttaatatca tcatctcctg cttcgtaatg
    41461 ccatttgata accagctgat ggcctttgct ttttaggtcc acaaactgat gaaacatatt
    41521 taacattgct ttagaggtgc cactattaaa gtattccaaa tctatattta attctgtcac
    41581 aggagccgga tgatgagaat attcttctat ccaactgttt attatatcga aaaactttga
    41641 aggatcttct ggtatcgaac gtcctttcag caacatttgt ccatcttttt ttaaatgaac
    41701 agtaggggtc ttctttgtgc cttctattag taagttgtcc atcattcatt aaattctggc
    41761 taaatttata actttttaga ataaaaaaaa agagtatatt ttattatact tttatgtgta
    41821 aatttttttt actcaaaaca tgataaattt atacatttat gtaccaatag caataaaata
    41881 gcaaatgaat gagaaaacat taatagaagc tgctctattt gctgctgaaa aacacaggtt
    41941 gcaaagacga aaatccttac tgtcaatacc ttatattaac caccctttag ctgtttgcta
    42001 tattttagcc caatcaggaa taagctctat taatgttttg gttgctgctt tactacatga
    42061 taccattgaa gacactgaaa cttcccctga cgaaatagcc aatctttttg gcaatgaagt
    42121 attaaaaatt gtattggaag ttactgatga caaaactttg aatgacaaaa aaagaaaaga
    42181 acttcaggta ataaaggccc cacaactatc acaaggggca aaacttttaa aaattgcaga
    42241 caaaatatct aaccttaaag acatcctgac ccacccgatg gattggagca acaaacgtaa
    42301 aaaagattac tttttgtggt caaaacaagt gtttgatggc tgcaaaggca taaaccctca
    42361 gcttgacaag ctctttgaag aaacatacaa cgaaggtatt gaaaaactaa gcagccttcc
    42421 cgactaaccg ttctttaaca taaagaaata ccgaaatcaa aatgtaaaaa acaataatca
    42481 caggaagtgc ttttatgctc atcaaaatca aacacaacaa acaaataaac aaaaataaaa
    42541 tctgaatgct atattttttt aaagaaaaac ttttcatttt gagcgaaaac ataggaaggt
    42601 tgcataccat cagaaatgaa tcaacaacag aaattgcaat cagccctatg taagtatatg
    42661 acaaagcccc caaccctaat aagctatcgg acgaaaaaaa gataatggtt atagaagcta
    42721 tcaggatagc acttgcagga gtaggcaatc caataaaagt gtcgccctga cgttcgtcaa
    42781 tattgaactt tgccaaacgt aatgcagaaa atgcaggaat taacgaggct acaataatat
    42841 ttacaaaagt actcttttca aacataagaa aattaaacga cgaaaattgc aacatttgat
    42901 acatcaaaac tcccggagca ataccaaaac taacaacatc agcaagcgag tcgagctctt
    42961 tccctatcac cgaattagac tttaacaacc gtgcagcaaa gccatcaaaa aagtcaaata
    43021 ctgcagctaa aaaaatataa tatggggcat aagcaatggt attgatgtta aatgcaaata
    43081 ttatagcggt aaacccggct actaaattta ataaagtaat aaaattagga atattcttta
    43141 caatctgttt cattgtattt caataataaa atttgccgac aaatgtaaat gttttatcta
    43201 taaaacgaag cttatgtttg tttaaaattc ggcattttta ttgtattaga ttaaatttga
    43261 aaaataaata attgagcaaa tgagcaaaag caaagtattt attcctgaat atttggcccg
    43321 agaatatcgc aaactgctaa agttactgcg ttcaacaaaa ggagatgttg atttttcaaa
    43381 aatcagggcc gcgttcacat tagccaacga tgcctgtaac tgcaacaaca gttattatag
    43441 tgaaccgctt attatgcaag ctgtacaggt agctcaaatt gttgcaaacg accttccttt
    43501 agggaccgaa caagtatgca tagcgctaat gttcaaattt gttgaaaacg gacttttgga
    43561 cgagaaaaat atagaagcct ctctgggcaa gcattatgcc aaacaaacaa aatcattggc
    43621 agaaatctcg aaaattaaca cccaaaaaat atccggacaa gctgagaatt tcagaaaact
    43681 gctcttaacc attaccgatg atgtgcgtat tgtgttggta aaacttgcag acaggcttta
    43741 tgttatgcga aaccttgaaa ctgaagaaac agaggtacaa aagcgcatag ccaaagaagt
    43801 atttgacctg tatgccccca ttgcccatcg cttaggcctg tatcatgtca aaactgagct
    43861 ggaagagctt gctatgaaat ttactaatcc ggacatgtac aattacatag aaaaaaagct
    43921 acaagacact gcttcagctc gaaacaaatt tatcaaagaa tttatcgtac cggttaaagc
    43981 aatactcgac caaaacaact ttgtatatga aataaaaagc cgcacaaaat ctattttctc
    44041 gatatacaat aaaatgcgaa agcaaaatat tgaatttgag gaagtttacg atttatttgc
    44101 catacgtatc attttaaaaa gttcattaga aaatgaaaag tccgattgct ggaaagtttt
    44161 ttcgttaatt accagtatgt acccgcataa ccccgataga atgcgcgatt ggattactgt
    44221 gcctaaatca tcaggatacg aatccctgca tgccacggtt atgaccgaac agggtaaatg
    44281 ggtagaagtg caaatccgta ctcaaaggat ggacgagata gccgaaaaag gatttgctgc
    44341 acactggaaa tataaaggtc aaaaagctga tgggacaatt gacacctgga ttaataaaat
    44401 cagagaagca ttagaatctt ctgcatctga ctctgaatct gcaattgaca atcaaaaatt
    44461 aagcctttac tctcaagaaa tatttgtttt tactcctaaa ggcgatttgc ggacactacc
    44521 taaaggcgcc acagtgctgg attttgctta tgaaatccat acaaatgtag gaaatacctg
    44581 taccggagct aaaataaacg gcaaaaacgt tcctatccgc tatgaactaa agaatggaga
    44641 taaagtagaa attattactt ctaaaaccca aacaccaaaa aatgattgga ttgaatttgc
    44701 cgtaacaaca aaagcacgtt caaaaatcaa acttgccctc aaaaacgagc ttatcctccg
    44761 tgccgaagcc ggaaaggaaa tattcaggcg caggctgcgc aattggaaaa ttgggttcaa
    44821 cgacgtgttg gtttcaaaat tgatgaaaca tttcaaaatt aaaactgcca ttgactttta
    44881 ctccctcatt gcagatgaaa aaattgaaat gacagaaata aagaaaatca tcacagcatc
    44941 ggaaaaaaca gctaatgaat cgccagaaaa aattgacaac gcccaattag acaagataat
    45001 accttcttta gccgtaaaaa gtgaagaagt attagagata gacaacaaaa tcatcaacca
    45061 tgttgaatat aaactggcta agtgctgctc tcctattttt ggggatgata tttttggctt
    45121 tatcactatt aatgacggca ttaaaataca ccgagtcact tgtcctaatg ccagggacat
    45181 ggtaaaccgt tttggatacc gcattgttaa agcccgttgg tccaatacag ataataacac
    45241 cttttttcag gcagctttgc gagttacagg tagcaacgaa ttgggcattc tttcaaaaat
    45301 tacagatatc atagcaaaag acctgaaggt aaacttgcgc tcaataaacg ttgatactca
    45361 ggatgttgtt tttgaagggg taataaaact ttttgttaaa gaagtatcac acttagatat
    45421 gctgatacac aaactcatga aagttaatgg tgttgatgtt gtacaccgta tagaagcttg
    45481 atttttactt aatttttaag gccaaaataa ataattgtag ccatcagcat ttcatctcca
    45541 tcactacata tttcataatt tacttcacca ttacgtttgt gaacaacctg cacaagttca
    45601 tgtgtttcat catagaccaa ttggtatgcc cccttggtca gatgcgtctt tgatgcttct
    45661 attagttttg taaacatgct tacttcctgc attttgggga tgccttattt tactgtaaaa
    45721 gtatatcgtg caaaccttat ttacctttta atataacatt tgttatcaac aacttacatt
    45781 atattattaa caatcccgaa ggaatgaaat ggataatcat aatacatcca ccgtcaattc
    45841 aagtttcata gctcttgaac cttttacaag aaccatagca tctttaattg gattctggca
    45901 aatataatcc actaaagcat tcgtattttc aaatgttttg aagttgactt gttttgcagt
    45961 ttctatgaag gaattaccaa caagatatac ctcgctgaat ttcatttctg aaagtaaatt
    46021 aacaatcttt gtgtgttctt ctaaagaata cttgccaagc tccttcatct ctccaaggat
    46081 aacaatcttc ttttctctct tcccggtttt taatatggca aaatttcgta tagcagcttc
    46141 tacactgacc ggatttgcat tgtaataatc acacagcaca gtattaaagc ttttttcaac
    46201 tatttgcgag cggttattct gcggaacata ttctgctata gcttcacagg catctgcaaa
    46261 tgcaactccc acatacatag caattgttat cgctgccaat atattttcaa gattataact
    46321 tcctgtcaaa tgagttttaa caatgcgttc actaccctta caatatattt tgcataccag
    46381 tgtggggttt acttctacaa tttctccata aatttctgtt tttgcagcat ccccaaaacc
    46441 gatcttttga caactgacct ttgctacttc cgatacaagc aaaggattat tagcattgta
    46501 aaaaattata ccattttcct tgttattaag gtaccggtac atttctgttt tagcagtaac
    46561 caccccttca aatgaaccaa aaccttcaag atgggctttg ccaatattat ttataatacc
    46621 taagtcaggc tctgcaatac tgcataattg agcaatttcg ccaatgtggt tagcccccat
    46681 ctcaataatt gccatctctg tatcaactgg aactgaaagc aaagttagag gcacacctat
    46741 atgattgttt aagttgcctt tagttgcaat tgttttaaat tttttggaca aagttgccaa
    46801 tgtaagctct tttgtagttg tttttccgtt agttccggta atgcctatca cttttgtatt
    46861 tagatgcttt ctatgataat tagccagttc ttgtaaggta ttcaacacat tttcaaccaa
    46921 aaaacattta ggatggttta ctttaacatc atcaaccaca gcatatgcag ctcctttgtc
    46981 aagtgcctca aatgcatact tgtttccatc aaaagactct ccctttaaag caaaaaaaat
    47041 acttttaggg ataatatttc ttgaatcggt agaaattaag ggatattgtt gataaattaa
    47101 atataaatca ttaatattca taagtctgta ataaatctca attgattaaa ctaaaaagaa
    47161 aaagccttac actcattagc gtaaggcttt tatatactta attgatttca ttaaaatcct
    47221 ttagggctac ccacacgggt catagcgcaa cggaacccaa tatcatcacg cgaatctgtt
    47281 tcattaagga acctgcgggt tccaggaaca agccagtatg cacggtcttt ccatgaacct
    47341 cctttgtaaa cccttacatt gtcagtaata agagaagaca tatcaccttg accctgagca
    47401 tacattcgtg tagagccgcc aggattttga gcagatgttg tcgatgttga aaaatctaag
    47461 cttgattgaa catcaccatc gagataattc ctataatcag cttgtttgta attcctacga
    47521 ttggcagcat tagccggatc aacaggttta aattttatac ggcctaaact gtcctttgga
    47581 gctacgttcc catcattatc tctttccatt tccataaaca cgttaccacg gaaaggcctg
    47641 aattcatcat tgtcttcctg agtaagaggc ctgtaaacgt ccatcaccca ttcactaaca
    47701 ttaccagcca tacaataaag cccataatca tttggccaat atgacttaac aggagcagga
    47761 acatcagcgt tatcattaag gtctccggct gtacccatat agtcccctct gcctcttaca
    47821 aagttagcac gcatttgtcc acggctttta gcatcgtcat ttcttaacgt atgaccattc
    47881 caaggaaata atctgcgttc gtaaatacgt tcatcaaaac tgttaccaat aagagaaaat
    47941 gctgcaaact cccattcagc ttctgtagga agacgatatt taggcaataa caaaccatct
    48001 tcaagtgtaa tttttctttc aacacccatt ggcgaaagtt ttggattctc accaggaagg
    48061 ccatcatatt gcccaactaa ataagcatca gtattaaagt tttcagcacc agattgttgt
    48121 ggatccattt ttaagtatcc gttatcaatc attatttttt catttacacg gtctgaacgc
    48181 catgcacaat agtcatttgc ctgaatccag ttaaccccta caacaggata ctggttatat
    48241 gcaggatggc gaaaataata ttctacataa ggttcattat atgcaagttt acttctccaa
    48301 actaaagtgt caggcaaagc atttttgtaa acagcaggat actcagcgaa cactcttttt
    48361 aaccaaaaaa gatactcgcg atagtccaca tttctaactt ctgtttcatc taagtaaaaa
    48421 gaagaaacag aaacac
//
LOCUS       DAOSPF010000023        47966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_798_length_47966_cov_7.238317, whole genome shotgun sequence.
ACCESSION   DAOSPF010000023 DAOSPF010000000
VERSION     DAOSPF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_798_length_47966_cov_7.238317"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..825)
                     /gene="cbiM"
                     /locus_tag="PK252_06370"
     CDS             complement(154..825)
                     /gene="cbiM"
                     /locus_tag="PK252_06370"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOT14369.1"
                     /translation="MHIPDGYLGPKTYITMYAIFTAFIFVAIKKLKKKLSLKLIPFLG
                     FASAFSFLIMMFNIPIPGGTTGHAVGAALAGLVIGPWAAMIAVSVALIIQALVFGDGG
                     ITAIGANCFTMAIVMPFIAVWTYKLIAGNTDNKRRTGVAAFFAGYVSLNVAALITAVL
                     FGLQPIISTDAAGNPLYSPYPLKIAVPAMTLEHLLLFGIVEGIVTMVFFQYLKKANPE
                     LVSNK"
     gene            complement(840..1958)
                     /locus_tag="PK252_06375"
     CDS             complement(840..1958)
                     /locus_tag="PK252_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOT14370.1"
                     /translation="MKKFEAILLVLAIILKSNNAVSQEESNNKKQIEPIFSYTADAAR
                     NFSGGIKKGNAYIGLIDAGVILHTDKYWKNGEFTIELQNGHGENLSENYIGDAQLVSN
                     IENGNYTYLYQAYYRQSFENGYIFSGIHDLNSEFCTSEYGASLTNSSFGMFSTFSLNF
                     PVSMYPKTSLALAGCYKQNENIRHRLGFYDGDAGSLDDEKYNLSWSISANQGLLTIYE
                     LECNIPSINQVLKIGSYYHSGKFYNTSRTDSVIKGNYGFYGIIDQTIYKNNEKTLGAF
                     MQISYAPDKININDWYAGFGVSYSGICPKCIQDALSLGIALARIDKKIETDLELNYHI
                     YVCKHLSLQPTIHYVINPGVIARLDNAFAAYLRLNVVL"
     gene            2197..4089
                     /gene="speA"
                     /locus_tag="PK252_06380"
     CDS             2197..4089
                     /gene="speA"
                     /locus_tag="PK252_06380"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOT14371.1"
                     /translation="MRKWRIEDSAELYNINGWGINYFSINEHGNVQVTPKKNGVAVDL
                     RVLVDELLLRDVATPVLVRFPDILDNRIEKMSNCFRIASEEYGYKGQNFLIYPIKVNQ
                     MRPVVEEIVNHGKKFNIGLEAGSKPELHAVIAINTDNDSLIICNGYKDEDYIELALLA
                     QKMGRRIFLVVEKLNELKLIANISRRLKIRPNIGVRVKLASTGSGKWEESGGDGSKFG
                     LTSSELLEALDFLEKNKMKDCLKLVHFHIGSQVTKIRRIKIALREASQFYVQLYKAGF
                     NIEFVDIGGGLGVDYDGTRSSYSESSVNYSIQEYVNDSISTLVDVSDKNGIPHPNIIT
                     ESGRSLTAHHSVLLFEVLETTSLPTWPEDEEVQPTDHEHVQELYKLWDNLNQTRMLET
                     WHDAQQIREEALDLFSLGLIDLKTRAQVEKLFWSIAKEINALSTDTKHAPDELRQLPK
                     LLSDKYFCNFSLFQSLPDSWAIDQVFPIIPIQRLDERPDRSATLQDITCDSDGKIDNF
                     ITSKSFSYYLPVHSLRNKDSYYIGVFLVGAYQEILGDLHNLFGDTNAVHVTVNEDSYE
                     INQIIDGESVAEVLDYVQYNPKKMVRTVETWVTSSVKSGIISAEEGKEFLSNYRSGLY
                     GYTYLE"
     gene            4160..6097
                     /gene="thrS"
                     /locus_tag="PK252_06385"
     CDS             4160..6097
                     /gene="thrS"
                     /locus_tag="PK252_06385"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT14372.1"
                     /translation="MIKITFPDNSVREYPKGSSALDIARSISSNLAAEVLSATVNGEL
                     IDASRAINTDASIKFHKWDDEQGKHAFWHSSAHLMAEALEALYPGIKFGIGPAIEQGF
                     YYDVELPNGQNITDADLPKVEQKMLELARQKSVYQREEISKSKALELFNQKGDPYKVE
                     LITELEDGTITLYRQGNFTDLCRGPHLPDTSFIKAVKLTSIAGAYWRGNEKNKQLTRI
                     YGITFPKQKLLEEYLVMLEQAKLRDHRKIGKELELFAFSEKVGMGLPLWLPRGAALRD
                     RLEQFLRKIQKQHGYQQVITPHIGQKELYVTSGHYAKYGKDSFQPIHTPAEGEEFLLK
                     PMNCPHHCEIYKTKPRSYKDLPIRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHLFC
                     RPDQIKEEFCKVIDIILYIFKILDFKDYTAQISLRDKENRDKYIGSDENWDKAEQAII
                     EASAEKGLNTVIEYGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFELEYIGS
                     DDQRHRPVMIHRAPFGSMERFVAVLIEHTAGKFPLWLTPDQVVVLPISEKFQDYAEKV
                     LNYLNNSDIRTLIDTRNEKIGKKIRDNELRKIPYLLIVGEKEAENETVAVRKQGEGDK
                     GVMTLDEFVQHIKQEIERQLS"
     gene            6132..6698
                     /gene="infC"
                     /locus_tag="PK252_06390"
     CDS             6132..6698
                     /gene="infC"
                     /locus_tag="PK252_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT14373.1"
                     /translation="MVGKSNTPGPRPVQQKVVEKQHRINQEIKAKEVRVVGENVEGQN
                     VMPLQEALRLADELELDLVEISPQAVPPVCKIMDYEKFLYQQKRKQKEMKAKSVKVVV
                     KEIRFGPNTDEHDYNFKLKHAQSFLQEGAKVKAYVFFKGRSILYKDQGEILLLRLAND
                     LEELGKVEQLPKLEGKRMSILINPKGKK"
     gene            6722..6919
                     /gene="rpmI"
                     /locus_tag="PK252_06395"
     CDS             6722..6919
                     /gene="rpmI"
                     /locus_tag="PK252_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT14374.1"
                     /translation="MPKMKTNSGASKRFSLTGTGKIKRKHAFKRHILTKKTTKAKRNL
                     TYFGLVSPADEKNVQLLLCMK"
     gene            7004..7348
                     /gene="rplT"
                     /locus_tag="PK252_06400"
     CDS             7004..7348
                     /gene="rplT"
                     /locus_tag="PK252_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT14375.1"
                     /translation="MPRSVNSVASRARRKKILSRTKGNFGPRANVWTVAKNTWEKGQQ
                     YAFNDRRKKKGEFRALWIQRIGAAVRPFGLSYSEFIGLVNKKGVALNRKALADLAMNH
                     PVAFEAVVNAVK"
     gene            7535..8251
                     /gene="dapB"
                     /locus_tag="PK252_06405"
     CDS             7535..8251
                     /gene="dapB"
                     /locus_tag="PK252_06405"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT14376.1"
                     /translation="MKIALIGYGKMGKMIEEIALLRGHQISYRIDIDNIEELDQLNPS
                     NTDVAIEFTTPHSAYGNVMKVLKNKVNVVCGSTGWNDKLPEARKYAESNNLGFLWASN
                     FSIGVNIFFKVNEYLASLMKNTDGYTPEMTEIHHIHKKDAPSGTAITLAEGIIKHLPT
                     INTWKLAPDNASDAVTIEAKREGEVPGTHIIKYASDVDYIEITHCAHNRRGLALGAVV
                     AAEFLKGKKGNFSMTDVLNF"
     gene            8417..9808
                     /gene="lepB"
                     /locus_tag="PK252_06410"
     CDS             8417..9808
                     /gene="lepB"
                     /locus_tag="PK252_06410"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT14377.1"
                     /translation="MKSFLKLLKNKYFKFGVAITIYLLWVIWLGNYWFLLGAPIVFDI
                     YVSKKVNWTPWKKRSGKNSKLVEWIDAIIFAVVAVTIINIFIFQNYKIPTGSMERSLL
                     IGDHLYVSKLSYGPRIPNTPLAFPFAQNILFGTVPSFSTIIQWPYKRLAGFGKIKNDD
                     IVVFNYPEGDTVALEMPSNQSYYDYINMRIEELKGFDERNNVKRTDEEYYAQARKEVW
                     DRYTVVWRPVDKTDNYVKRCVAIGGDTLQVIDGEVFINGKPQKDIDGKQFVYYVKTNG
                     QPFNSKVLEDMDIYDSDIQYSGDGAFMPLTKLNYEKVRHLSNVTGIEPIFRGKGECDL
                     RVFPHNKAFAWNQDNFGPLYIPKQGDSIALTPKNLILYKRVIAHYEKNKLEVKDSIAY
                     INGKPSKYYTFKMNYYFMMGDNRHNSLDSRYWGFVPDDHIIGSPVFIWLSLNQNKSFP
                     GNIRWSRMFMLAK"
     gene            complement(10116..12779)
                     /locus_tag="PK252_06415"
     CDS             complement(10116..12779)
                     /locus_tag="PK252_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT14378.1"
                     /translation="MNIRNFRQAFITTLLLFFSFSVGIAQLKKHIPPVNKELTEFYQK
                     KKSLRAAIDEKTTGYIPPYTQLSEYYIENEESSKLKSAMVSGLPSVFDLRKIYNETSQ
                     KYDNLVSPVKNQGSGNTGGNCWAFAALSSIESMWYNPLSAINALDLSEQNMATCHGYS
                     WAFGAGGNEFFAQAYLSRLQGAVLETDVPYNTTDISTFTCKKAKPVAYFPESRRIYGN
                     IPMIKKVIMDYGAVATNIHWDTAYYKKKTNIFYSTDRTGPNHAISIVGWNDTITTQAG
                     KGAWIAKNSWGTTWGASGFFYIAYGDKQILKPASYYPTMWATESVDTVFKYNNIGVVS
                     FFGYEKESNYALLKYTSASKKLLKSLSTFLPVTGSSLTIDIYSDSLSTLIHHSVNVPV
                     QCPGTYAFDIMAEVEGTFYVKLTYFTPGVNYSIPVELKTDVNGEEYANPFVYPSGYQW
                     VSANGNIWQALGSDVVGWEANIIAHLYAITPSKPVAVFTTDKYEVCAGSTVTYTAKTN
                     GNINKYLWVFGEDASPAYATGAGPHVVTYSSNAKSGLRYPALYVIGGNDTVSFSKPIK
                     VVNSLTTLIAAPTYIKQSDSAILTAIVDADSYNWYPSTNLSDTFSKSVVFKADNPGLY
                     KFTLTAKQGNCQGQYSLNVYLKNPPSNDKPCNAITLSPGENGPFSNVGATADYNEVHP
                     ADTNCYLDKTWCNEGGVQNSVWFKVIGPDNGKLSVNTRGMDNQLALYSSESCDNIKFE
                     DLVAANDDYYGIEDNYAAALDEITVIPGKTYWLQVDGSFGGVEGEFYITITDTEITTA
                     INQISTSENLTLFPNPGKGVFTVHYKSDYTERLKIRIFGINGTLVYQSNEYKTPQEFS
                     KQINLNKLSKGVYFINVQSEHSSNTVKYIAQ"
     gene            12999..16301
                     /gene="secA"
                     /locus_tag="PK252_06420"
     CDS             12999..16301
                     /gene="secA"
                     /locus_tag="PK252_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT14379.1"
                     /translation="MSIIENLIRSFFGSKSDRDIKEIMPIVAKILAEYDKLKNLSNDE
                     LRALSSNLKNEIRDLIKTENEKVDALKQQADNPELSIEEKEDIYRDIDKLEKQIDTLV
                     KDKLDEMLPLAFAIVKDTARRFCENEKLEVTATDFDRNLASSRKSIEIIGDKAYYSKR
                     WMAGGNEITWDMIHYDCQLIGGIVLHQGKISEMATGEGKTLVATLPVFLNALAGRGVH
                     VVTVNDYLARRDSEWMGTLYEFHGLSVDCIDKHQPNSEARRKAYLADITFGTNNEFGF
                     DYLRDNMTSSPSELVQRVHNYAIVDEVDSVLIDDARTPLIISGPIPRGENHEFEMFKP
                     KVESLYNAQRKLVTQILADAKKAIDAGDKEKGGLLLLRAYKGLPKNKALIKLLSEEGL
                     KNLMLKTENFYMQDNNKQMHLVTDELYFVIDEKNNSIELTDKGIDLITTTSEDSSFFI
                     LPDIGSEIADIEKNITDPAKKLEKKEKLLQDYAIKAERVHTVNQLLKAYTLFEKDIEY
                     VVMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKIEAATQTFATITLQNYFRMY
                     KKLAGMTGTAETEAGEFWNIYKLDVVVIPTNRPVIRKDFEDKVYKTKREKYNAVIDEI
                     AELIEAGRPVLVGTTSVEISELLSRMLKIRGIKHNVLNAKLHQKEAEIVAEAGKSKTV
                     TIATNMAGRGTDIKLSAEVKAAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFF
                     VSLEDDLMRLFGSDRIASIMDRLGLKEGEVIQHSMISRSIERAQKKVEENNFGIRKRL
                     LEYDDVMNSQREVIYKRRRNALFGERLTLDISNMLLDICENIVERNHEAGDFEQFNLD
                     IIMNLAVESPFTEKEFKEMSVKEMIEKLHAGVWEAYKHKMNQIAQQVAPVIKDVYEKQ
                     GAQYENMVVPISDGIHVYQILFNLKKGAESNGAEMIKSFEKLAILMNIDDAWREHLRE
                     MDDLKQSVQNAAYEQKDPLLIYKFESFKLFRTMIENVNKQIVSLLMKGHVHSPEQNQP
                     QRQQAPHKIDLSKLNMSRSNSLPGSRPQMNTNREQQPMQPIKVEKRIGRNDPCPCGSG
                     KKYKNCHGQNAE"
     gene            16324..16605
                     /locus_tag="PK252_06425"
     CDS             16324..16605
                     /locus_tag="PK252_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14380.1"
                     /translation="MANKRTLKKDINYLTYELISEVLVYRFYHPEADQNVLNEILGTL
                     IENRNNLVGRINHINGKDNPKLVKQHFSSIRKDFQNSVTILDKLAKTKK"
     gene            16630..17979
                     /locus_tag="PK252_06430"
     CDS             16630..17979
                     /locus_tag="PK252_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOT14381.1"
                     /translation="MFERIRAKVLKEDNLVQFRKDHSDKKIVFCTGCYDILQSGHAVF
                     FNQCKEYGDILVVGVGRDAVITKLKGAGRPVNPENNRLYLVASLLDVDYAVLNGEEIR
                     PGKIDFYNIIEKLRPDVFVLNDDDSALAEKEVLCNKFGVKIELVSRVVPEELEATSTT
                     RIIDKINFSFKAPLRIDFAGGWADVPYIMGDETGYVTNVAIKPLIEYNKGKFNFSGYP
                     RGSGLSTSTAVKLLEMISAKNYNSDSKPLNVIAEDLFNLENTELKWAIGRQDQYAIVY
                     GGFNCFEFGKNYGKPVGTETDKEKLEKFRANLLLLHTGVSRNAQSAVEQVYLNFHTTE
                     GKAALKKISDCGKAFFTAFQQGDFMKCASIMEENFEAQKQLAKATSNEYLDEMYAFAK
                     QNGAYGGKICGAGGGGAFIFYCKDPVKLKQAMKHQFVDCFEIDFEFEYKSIKELNTI"
     gene            18170..19294
                     /gene="dnaN"
                     /locus_tag="PK252_06435"
     CDS             18170..19294
                     /gene="dnaN"
                     /locus_tag="PK252_06435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT14382.1"
                     /translation="MKFVISSNVLFERLQAINKVISSKNTLPILDNFLFDLSDKKLTI
                     TASDLESTMITSLDLENAEGEGVIAIPAKRLIDTLKEFSVQPLTFDVNTDTYATVILT
                     QNGKFTITGQNGDDFPTLARLKDDKKSVMVDANILFSGISKTLFAAAEDDLRPIMNGI
                     FMEIYSSGLTFVASDGHKLVRYRRTDVTVEEEGSFVLPQKPATLLKTVLAKEQGTVAV
                     SYDDRNAYFQMANYKMVCRLLEGSYPSYAAVIPNNNPNKMIIDRVEIYTTIKRVAVFS
                     NQASNLIKLQIANNQLIVSAQDIDFSISAFERLNCQYDGDEIEIGFKSQALLDILSNL
                     SSEHVVIELSDPGRAGIILPVEKDIEAEDILMLQMPLQIS"
     gene            19380..20186
                     /locus_tag="PK252_06440"
     CDS             19380..20186
                     /locus_tag="PK252_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT14383.1"
                     /translation="MELNLKNPLIFLDLETTGLNIVSDRIVEITILKVFPGGKEEVKT
                     YRVNPEMPIDPKATAVHGITDEDVKDCPTFKELAQTLVNEFEGCDFAGFNSNKFDFPM
                     LAEEFLRANVDFDLKKRKFVDVQVIFHKMEQRTLSAAYKLYCNKELVDAHSSKADTYA
                     TYEVLQAQLDKYQGKKFVDAAGNTSEPITNDIDFLSKFSAHNSNVDFIGRIIYNEKGV
                     EVFNFGKYKGMPVVDVLKKDPSYYSWMMNGDFPQYTKNVLTKIKLREFQK"
     gene            20195..20608
                     /locus_tag="PK252_06445"
     CDS             20195..20608
                     /locus_tag="PK252_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT14384.1"
                     /translation="MVAQHQIVLPEFRRGFHLVTSHIERNLPVLPKTGIVNLFIQHTS
                     AALTINENADPSVRADFRTFTDKIIPDGDRCFSHTLEGDDDMPAHIKSSVYGQSLTIP
                     VTNGRLNLGTWQGIYLCEFRDYGGPRHIVITVIGE"
     gene            20610..22067
                     /locus_tag="PK252_06450"
     CDS             20610..22067
                     /locus_tag="PK252_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT14385.1"
                     /translation="MREDSFNSLTPKKVWHFFSEICQIPRPSKHEKAIIAYLESFAKQ
                     RGLDYLVDKGGNIIIYKKATLGYENKPVIVLQSHVDMVCEKNADTTHDFFSEPINAYV
                     EDGWVKAQGTTLGADNGIGVAMQLALLDDIDIQHAALECIFTVDEETGLTGASTLEAS
                     AINGRTMINLDSEDEGIIFIGCAGGVETIGYCTPEYSNVPDNCAAFVLTVSGLQGGHS
                     GDDINKGRGNAIKILTRLLYQFQKHASFYMSDISGGNLHNAIPREAKSSFVIEKSKTT
                     VFVKETNDLIKHILDENKDNEPNLQIQVKETLLPEKVYTKDFSARLICTLYALPHGVV
                     AMSNTIPGLVETSTNLAFVRHTTDGRIEIGTSQRSALQTAKYDISQQVASVFILGSME
                     YVHTMGYPGWKPNKNSRILKHAEGVYQKMFGKEPVITAIHAGLECALFVDKIPGLDVI
                     SVGPTIKGAHSPDERLSIESVEKFWKYLIEILETW"
     gene            22098..22796
                     /locus_tag="PK252_06455"
     CDS             22098..22796
                     /locus_tag="PK252_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOT14386.1"
                     /translation="MQRKYQHLFFDLDRTLWDYDANAKETLCDIYEKYDLASKGVDLD
                     KFLHDFTRFNNILWAQFRHGEIKKEYLKVRRFYLTLKSSQIKDNDLAKAASDDYLSIS
                     PEKTRLIPGAMELLEYLSGKYHMHIITNGFTEVQFKKLRNCKLEKYFERIVTSDMVKA
                     NKPKKEIFHYALSGANAQKSTSLMIGDDWDIDIMGAKYFGFDQVYFNRDGKELKGKAT
                     YEIHSLEELITILT"
     gene            22819..23895
                     /locus_tag="PK252_06460"
     CDS             22819..23895
                     /locus_tag="PK252_06460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT14387.1"
                     /translation="MKKKFFYLVLFSLPILGCAQTKLIPFNSSNIYYEGRMLYKPEAA
                     VFNWSGNSATIIIDGTEISAVMKDLDTANYYNVIVDDSVIAKFHTDTVKKSYVLVSGL
                     KAGKHKVQIFKRTEWDKGKTLFYGFETNSNTKVLPPLPPKKRKIEFYGNSITCGYANE
                     DFTNQDRWFGYFQNNYLTYAAMTARHFDAQYSCVGKSGIGIVISWFPLLMPEMYNRLD
                     PTDSTSVWDFSKYTPDIVVINLLQNDSWLTNRPDHEQFKYRFGTKKPEVSFVIEAYKN
                     FVSSIRKVYPNAHIICALGSMDATREGSPWPGYIETAVKQLADPKVYTCFFPYKNTGG
                     HPKVSEHKVMANQLIDFIDKNIKW"
     gene            24118..24191
                     /locus_tag="PK252_06465"
     tRNA            24118..24191
                     /locus_tag="PK252_06465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24152..24154,aa:Arg,seq:tct)
     gene            24215..24898
                     /locus_tag="PK252_06470"
     CDS             24215..24898
                     /locus_tag="PK252_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14388.1"
                     /translation="MSEVNNEHSGIENVGEALSKAELFIEKNKKPIIIAVAAIVLVIG
                     GYFVFKKAYVAPMEKEAQEQMFVAEKYFEKDSFQLALNGDGNNWGFLKITEEYGITKA
                     ADLAHYYAGICYLQTGNFKAAIEHLEEYDGDDKLIAPIAVGATGDAYVELGDLKKGAK
                     LYEKAADMSENDFTSPLYLMKAGVIYENLKDFKKALAIYKQIKKDFPRSTEGRTIDKY
                     ITRAELLSK"
     gene            24914..25396
                     /gene="ribH"
                     /locus_tag="PK252_06475"
     CDS             24914..25396
                     /gene="ribH"
                     /locus_tag="PK252_06475"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT14389.1"
                     /translation="MATIYKPAYNEVPSGAGKSFGIVVSEWNPEITEALLDAACSTLL
                     KYGVKEKDIHVKRVPGSFELTLGAQFFAEYTEVDGVICLGCVIQGDTPHFTYVCQGVT
                     HGITELNLEYNIPFVFGVITTNTFEQAKDRAGGKLGNKGEESAIVALKMAALQDEMSK
                     "
     gene            complement(25399..25782)
                     /locus_tag="PK252_06480"
     CDS             complement(25399..25782)
                     /locus_tag="PK252_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14390.1"
                     /translation="MEFYLERTAHTPLIKFDKGKLLIAGRSIPEDITETYDPLFEAVA
                     NYAKHPEKHTEVSIMLDYSNSSTNRVLMSLFDYLNMYLFQNNLSVTVNWYYIKDDKEM
                     LDLGTDFKEIVKIPFALIEVDDFPE"
     gene            complement(25913..26443)
                     /locus_tag="PK252_06485"
     CDS             complement(25913..26443)
                     /locus_tag="PK252_06485"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT14391.1"
                     /translation="MLNKSEIRNSIKELKKKQSQDYYPVHSQIIADKIERLPQFVHSQ
                     TIAMYWPMPMEVDLTPLIIKYSTSKTILLPVVSDDDIVFSQFEGESQMKKNRYNILEP
                     TGTVFSDIEQIEALIIPGIAFDKSGNRLGRGKGYYDRFLSTSNAFKIGVCFEFQLFES
                     IPALPHDVKVNMVICG"
     gene            26578..29793
                     /locus_tag="PK252_06490"
     CDS             26578..29793
                     /locus_tag="PK252_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT14392.1"
                     /translation="MSKLLKINASAGSGKTFRLTREMLLLLTRNPENYKHILAITFTN
                     KAAAEMKSRVLKALLEINTDKGSVHLKALADLNKMPVEAIQKKADEALFNILHNYSLL
                     SVITIDSFFQRVIKSFAREMGFQMNFDIEMDLDSVLVSSAHEMINDLKTGDKLTEWLA
                     ELAIKRIEEGKSWSFEDDIVSMGKQLFNEDFKLFSETFHNRIKDRDWLLRYKQLLVQY
                     KKHIEVHTLKMLAVLSADAKRIMDKYQVGADSFKEKSRGIGTFMLSLDKYFVKDLPAC
                     VNKYKDDVSLWPQSDKVANSVIAAANAGLEKILKDAVNLIQVKKKSFNSCSLVLDNFY
                     TLGILSELSQKIDDYSKDNNLFLLSNTAYFLHEIIGNNDVPFVYEKMGQMYSHFMIDE
                     FQDTSIIQWNTLKPLIAGSLASGGKSLVVGDLKQSIYRWRNTDWRIMAQKVDEDVKEQ
                     GVKEETLDKNYRSDYNIVAFNNSFFFQAAESIQKSLTDEMVSVGFEDNIFKTMIKKAY
                     ESNFQLAKNQDKGLVKIDFIDTTEKEVEGEKVKLDFKAESLQLMLADLKLIGEKGSTA
                     ILVRTNKEGQEVMDFINENNRLEEDPTKRIAVISDETALLSNSVAVNFILNIFQYYIN
                     PVNVINKEELVNNWNIILSEMQANQAKVQWNELSVLLKENEHFRFLLPDRLLEKVIEL
                     FQLNQVTSYLPYLQRFYDVVNEYLHRSGSRHLLGFVDYWEEKKSKTSLSLTENQKAVR
                     IITLHKSKGLQFDNVLIPFCNWPMEYKNFDAPLKWVQGDYHLFKAIEHAPVQFSKAME
                     NSYFSKDYYIEKAQFYLDNLNLLYVAFTRPKNNLYLYCEFNESKSYTVSHLVNDVLRN
                     SNNIDGSIFPQVNLQKYWDKENMSQYQIGSYSVPEDKDAESIENIELYNLPVSAIDEH
                     LKSKPLVYDFASQQKASKRLSGNTMHKLFQYIYKAEDIDKAINKLVGEGLISSVDRQI
                     YSERVNSLVMKEPYKDWYCGDWKVYTEAAIVVPKQHEYRPDRVMINGKKAVVVDYKFG
                     FVQKDSYIKQVSDYKQTLLDMGFEHVDAFIWYVSLDKYVMA"
     gene            complement(29843..30835)
                     /locus_tag="PK252_06495"
     CDS             complement(29843..30835)
                     /locus_tag="PK252_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOT14393.1"
                     /translation="MIHYKELGLVNTKEMFQKAVKGGYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYAIPIVLHLDHGDSFELCK
                     SCIDMGFSSVMIDGSHLPYEKNIELTKQVVEYAHKYDVTVEGELGVLAGIEDEVQADH
                     HTYTEPDQVVDFVNRTGVDSLAISIGTSHGAFKFTPAQCTRNANGILVPPPLRFDILK
                     EIEKRIPGFPIVLHGSSSVPQEYVETINKYGGALKDSIGIPEEQLREAAKSAVCKINI
                     DSDGRLAMTAAVREVFATKPAEFDPRKYLGPARDELKKLYMHKTIHVLGSANKA"
     gene            31323..32252
                     /locus_tag="PK252_06500"
     CDS             31323..32252
                     /locus_tag="PK252_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOT14394.1"
                     /translation="MLTIWKQKHKLIEIDNFERNCWVNVTNPIPEEIDRLIEEFRIPE
                     DYIRDILDADERSRTEAEGRWLMIIIRIPVFRTDNGIPYITVPLGILISPHSIVTICL
                     YENDVIRSILYPVKTKAINLENKINFVLELFQRSSSQYLKYLKDINLRTNQIERDLER
                     STKNKELQNLLRMEKCLVFFITSLKSNEMLLQKLQRSRFASSEELDEDLLADVIIETK
                     QAIEMSQIYSDIQAGLMDAFASLISNNLNVVMKQLTSITIILMIPTLIASFFGMNLHN
                     FIEDSQYGFVSVVVGSLLLSIIGVLIFRRRQFF"
     gene            complement(32249..33733)
                     /locus_tag="PK252_06505"
     CDS             complement(32249..33733)
                     /locus_tag="PK252_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14395.1"
                     /translation="MKKLIGLIALCSVFSLSYSQNSFSSGYYIDGVFLSDMQGFSSAR
                     STALAGAYSSLGGDLGAIGQNPAGIGVFRTSQFSFTPAFSSVNTNSTFSGQENTDFIY
                     KMNLHNIGVNMNINTDNGSGWQCINLVLGYNQLANYSGSYKISGLTTGTSMANEFINF
                     ANGSKVNDLNSYFEQLAYRLIVIDADSGSTSYHSAYDNIPLKQQHTLEKNGSVGTYYF
                     GMGTNYDHKLYLGFNFNIISGNYQYKTNHTEIDINDLITDNSFSFGYNLDTWVKGWNL
                     GIGVIYRPIETLRLGLSIQTPSFIKITQETSTRMTATPDNGKTFVEVPTDEYGYELGA
                     MSESYDITTPAKYTAGISYLFEDKGLISFDYEIEDFTKIKFSNSDYGADYFSTENSEM
                     KDNMRIAQTFRLGAEAKLNKFYLRGGCAYKQSPYSSGMLNSDAYTMLYAAGFGYNTGN
                     TYIDFTYSLQTVSENYLMYNHSALPKAELETSSGKFLLTVGFKF"
     gene            complement(33779..34975)
                     /locus_tag="PK252_06510"
     CDS             complement(33779..34975)
                     /locus_tag="PK252_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14396.1"
                     /translation="MKTIQFKISGILASVILLGFGLNTIAQDDLYYMPSKDKSNAVVA
                     VPAVDTSGMTNYEKYRALRDGDYSKSNVKAKNNTAEVANQPDLTTITYDSSNVYVNNY
                     YVQEDPDRFWRYRSFYFDYYYPYGYYYYDPYFYNYGWGYPYFGFSYGYYSWSLGFYWY
                     YPYYYGYYGFPYYHGYYHGFYDGYYYSDNYYRPRGNYNYTPGRRAGGFANINDKSTKY
                     YGGRYSGADFYHGTRRGANYLTNQYSGTNGISRRDVITTAENNTAVSRRSATSSTRYT
                     QYDNTQSSTRRYSNTPAYTLPPSQTSNNNSVVTRRYSNSTDNNTYNRRQSTSSENNTD
                     VNQAPRRRSESYTPSSNSSSGNYSNSQPVYRRETNNSNSGSYSAPTNSGSSRSSSGNS
                     GGGRRR"
     gene            complement(35035..37827)
                     /gene="uvrA"
                     /locus_tag="PK252_06515"
     CDS             complement(35035..37827)
                     /gene="uvrA"
                     /locus_tag="PK252_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT14397.1"
                     /translation="MQKQYNQSITIKGTRVNNLQNVTVEIPRNKLVVITGLSGSGKSS
                     LAFDTLYAEGQRRYVESLSAYARQFLGRMQKPEVDFIHGIPPAIAIEQKVNSVNPRST
                     VGTSTEIYEYLKLLFARIGQTISPKTGNVVRKHSVTDVVNFILSLPLDQKILLIAKVP
                     LQDKNIEGQLLEFKKEGLSRMLVGEDIKQVDDIEAIAHAKKYLTINLIVDRLITSDNE
                     EFRERCGDSTQSALLIGKGQCFVFTLSDRKWNEFSIRFEEDGVAFEEPSVHMFSFNSP
                     LGACPVCDGYGKTIGIDPELVIPDKSLSVYEDAVACWRGESSGQWKQDLINAASKFDF
                     PIHRAYSDLTKEQIDVLWNGNKYFEGINNFFKYLEESSYKIQNRVMLARFRGKTTCPA
                     CLGSRLKPEAHYVKVGGKSISELVLMPISALKAFFDTIQLTDYENKVAGRLLTEIKNR
                     INFLLDVGLGYLTLNRTSSTLSGGESQRINLATALGSCLTGSLYILDEPSIGLHSRDT
                     DLLIKVLQNLKNLGNTVVVVEHDEEIMRAADHIIDMGPFAGRLGGKVIFEGNHQQLEK
                     NKESLTAAYLTGKEKIEVPHSRRKWNSFIKLTGARENNLKGIDVKFPLHVMTVVTGVS
                     GSGKSSLVKGILYPALSRVLNAEIAKPGNFNKIEGDIHLISGIEYVDQNPIGRSSRSN
                     PVTFLKAYDEIRKLYASVPEAVHNGFTPTHFSFNVEGGRCEVCQGDGHIKVEMQFMPD
                     VTLVCENCKGMRFKDDILEIRYHGKNIYEVLEMTVEEAIQFFSDKKGATQNKIVEKLQ
                     PLFNVGLGYIKLGQSSSTLSGGESQRVKLASYLSKENSLQGPTLFIFDEPTTGLHFHD
                     IKKLLDAFDALISHGHSLIIIEHNPEVIKYGDWIIDLGPEGGDNGGNIVFEGTPENLI
                     NERNSYTGRFIKNKLQ"
     gene            37994..38581
                     /locus_tag="PK252_06520"
     CDS             37994..38581
                     /locus_tag="PK252_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT14398.1"
                     /translation="MNYTVFSDQELINSFIAGNNEALEVLFLRHKDKVYSYIYITVRN
                     KSLAEDIFQDTFIKVLNSLKEGRYNEHGKFLGWVLRIAHNLIIDHFRKVKQMSFVSDD
                     ILSGCSSHNKNHFDKNVEDAMVKEQIYSDAKRMIEFLPAEQREVVLLRIFGDLSFKEI
                     ADMTQVSLNTALGRMRYALINLRKLAEKHKIILEE"
     gene            38652..38831
                     /locus_tag="PK252_06525"
     CDS             38652..38831
                     /locus_tag="PK252_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14399.1"
                     /translation="MQQTSTLFFIEDNTDDSHIEFMHSLLLNSEFDGFISVDELTYQA
                     PQRVTNYVLQLIRNS"
     gene            complement(38880..39896)
                     /locus_tag="PK252_06530"
     CDS             complement(38880..39896)
                     /locus_tag="PK252_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14400.1"
                     /translation="MRKIFLLSSVLLASSAIFFGQTKLTSEKHSFRPGDSHHFNLMKN
                     ANEGLAGENVIWDFTTLEKSDKDLTSHMYAPENIASDVKIDNSNFVLEEFGNLFYFNT
                     SKDKMLQYGSVCGNTVTRYDKPFLKLKFPFSYGNKVTGYYSGTQTSGNSSVAINGQYE
                     IYGDATGTLLLPNDVTIDNVLRVKQVRTIEYTNSTPIKEITYRWYAEDLRYPVLVIIK
                     YVTQAQEYTAETALFANVTTSKKSPTAVETPEFISTTKVFPNPCEEVINISCKLSSQQ
                     KLSVDLYDASGKFVKSIISKQKYSAGDVNLSLSTEELGMKYGIYYLKISNNERSYTHK
                     IIKQ"
     gene            complement(39982..40389)
                     /locus_tag="PK252_06535"
     CDS             complement(39982..40389)
                     /locus_tag="PK252_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14401.1"
                     /translation="MNDAAILNGKKVLIVEDNQLNLLIAKRCLDSFGMIYSIATDGQS
                     AITLLGQENFDVVLLDLNIPVLDGWEIASFIRSSAEKNQNTPIIVVSASEDPNLENKL
                     KASKINAAIYKPYTPEQLKKALIENFVPVSSSI"
     gene            complement(40393..42951)
                     /locus_tag="PK252_06540"
     CDS             complement(40393..42951)
                     /locus_tag="PK252_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT14402.1"
                     /translation="MKFFLKKKKYSLAFLFSVKNRYDLFKNRLILQFSAFLLVISIFS
                     NLFLYYSSSEIFRFISMPYLITSLIMLVIAFKTKNIRWISYSTLTLLLISVNFSFVLF
                     GYSASAPAILWPLVIIIYAFLILDSKYRFYTAIAQLAFCSFIMFAQDRLFEVPYKYNP
                     VPGFLYDISSLVPAFLIIYFIYYFIKIEKSLQDSLFLKNQQLLKRNQSLANLKNKIAN
                     DEKQFRVLTETTNDIVMICSPEGKIKYVSNSTCNTLKSSKEKLIKSNFADIVHPDDKE
                     LISAELDKLSIKQNIDKVVHFRINNEKGNIIWFEGILQSLSDTSGDVTQIHVVASDIT
                     EQKQQQSKSHILNQVLTQSNEGIILIDKEGKIIYSNKESSELYGFMLADMLHLSIFDL
                     DESLNDINKWKKFFETLKNEKKINHDITFKRQGLVKIEMEERWEFHEVEGMEYAIRFL
                     SNIDERKKNERKIRKNLEKQSLLSEISFILNTSDNFDYKINESLRIAGNFLKSSRVLL
                     FQNILSGKAFSCTHEWYSPDLNPKRNEWQAIPYSMISNIHQKLMNKELVEIQLSNIQD
                     NEILNIFHPDDFLKTVLNPIFVSTSMSGFIAIVDNNCATWNESDKQFFITFSTILQNT
                     FKNHDAIQSLKMSERRFRELAELLPEMVCEASINGKITFANKQMQIQFGFNENDLSKG
                     KIFFNLFTQVDKQRLLENFEKMLRSEHIENIEYTIVDREGNEIPVMVYINIIMKEHMP
                     IGLRAVMVDISRRKNYERQLLSLAGITDDSPVAFMRVDRTGNVIYSNKQAETIKQFIS
                     DNYHSSFYDIFNSIYTKQSIEEMNLEIYNEQYWFSLYPDISSNYMTICARKKMQ"
     gene            43198..44601
                     /locus_tag="PK252_06545"
     CDS             43198..44601
                     /locus_tag="PK252_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOT14403.1"
                     /translation="MNKPYIGLFFVFCFVSIQMFARERKIDTLAYIPDEDEYNLVVSA
                     IKGDIPTAKSLIDKGINVNTSIDENMTPLVYAAMGGQEKMIVYLIGKGAEVNYRATQG
                     PTPLVAAVRSKHRSIVDLLISKGAEIDIPDANGRTPLMYSILSEDTLLFEKFIKAGAD
                     IHHKDTTGIDVLMSAVINDQKYFVQKLIELGATADTYDENNVTPLMVAVSRGDIEMAT
                     ILLDNKADINHKSHNQQTALTVAIENMDEQMVDFLIKRGADVNQKLTFAEKPSTVANY
                     FNVNAFIKDALYSAGAKKTWLPDYRKFVAGPLILGNMDELFTGIEVGLKDYRYNSDLL
                     LGFIIRPFPIRVQADFEGRTYQFWEGRSAFFLGIEKRLALIRKANDQQLGFLFGAKGA
                     YTFGNYRGTTLDFENSIVPMFNAGVYVSNRYLQIGLSYVYTNFKAKTVSNNRMELSVR
                     LYLGKAFNFSKNDYSPW"
     gene            44652..45029
                     /locus_tag="PK252_06550"
     CDS             44652..45029
                     /locus_tag="PK252_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14404.1"
                     /translation="MVSCSNETVGEDYYCVDCISEEPDTAQLIVKVSYQQDKDSIKLL
                     VYNNKFSLDTKLDTILNDTIAKDVLKFYVKVNEYYSAEAIYYVGGKEIHAIDGGWFET
                     KKIEGCQNTCWKLIGGDYDLRLK"
     gene            45037..46140
                     /locus_tag="PK252_06555"
     CDS             45037..46140
                     /locus_tag="PK252_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14405.1"
                     /translation="MYLHLKKRNTFFIICLFLCMMTAGFFSCKFDENPDEYLFTTKEV
                     TKDIDSLDVWTKSNIYIIRKKDFTVKGRLIIQAGTIIKFDPAVKPSIIITDSGSLSCM
                     GTNASPIMFTSLDDDEKGGDSNNDKQPRKPAAGDWGEIIIRSKELAVINNCYFLYGGG
                     STDKSTIVADSAAVDISNCVFGYNNGGNLYEGNGVLNLLKAKTGTRVRNCYFHSGNLP
                     MVIASNFSIDNTNQFHNPKDSTQKNNYNAIKIAGNQAVQGQVIWNEENVPFYVSTDIL
                     EISGNNNLTLGNNTVLKFKAGAKLLLDNGYSSLLNADGPGVFLTSVYDDYRKGDTNGD
                     KNQTLPANGDWLIKIYSDSAAFNRSHLYYFVEN"
     gene            46148..47482
                     /gene="tilS"
                     /locus_tag="PK252_06560"
     CDS             46148..47482
                     /gene="tilS"
                     /locus_tag="PK252_06560"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT14406.1"
                     /translation="MFERFEKYLKSFCKCLPTDKIIVAVSGGADSMSLLHMLYKAKYD
                     IIIAHCNFQLRGEESDNEEQLVRQTAAKLDIPIYVKRFETTEYASLKNISIQMAAREL
                     RYSWFENLRRNLSYHKIAVAHNADDNIETFFINLIRGTGIAGLTGMKAVSGNIVRPLL
                     FATREEIEAYCQTENIVFATDSSNLSDKYLRNNIRHNIVPLLRQMNGVFDQTMLDNLK
                     KLQGAYAIYSDAIKNIINEIVSASSESMILIDIRALLNTVEPSTVLFEILKEYNFSPV
                     VVSEIYNSLDADSGKQFFSESHWLVKDRDKLIVSRLSEDKANNEDVFYIFEDTEHINI
                     PISMSFIKSSAEGYEIKKNPNIAALDYDKLVFPLILRHWRHGDYFIPMGMKGIKKVSD
                     FFVDKKLSLPEKESVWLLFSAGELVWVTGMRIDERYKITNGTQQVYEVEVGE"
     gene            47576..47911
                     /locus_tag="PK252_06565"
     CDS             47576..47911
                     /locus_tag="PK252_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HOT14407.1"
                     /translation="MSDNNQYEKIIASIATQKEAWILQTDEGLIAMFEDNDMNSYIPV
                     WTSKEQAQKSADEDWEGYTAGRMGIGEFVEWMQELKDDEILVGLYSDANQAFSVDPME
                     LKKQLLAKK"
BASE COUNT        15170 a         8278 c         9616 g        14902 t
ORIGIN      
        1 gatgattgca aatgcaacaa cccacttatt tttcaatatt ttcaacatat aatatgacta
       61 attacggatg actaatgaca aattaaatta ctaattacag atgatataat ggatggtatt
      121 gattgataac tcttaatatc tcctttttcc ttttcactta ttacttacta attcagggtt
      181 tgcttttttc aaatattgga aaaacaccat cgttactatc ccttcaacaa taccaaaaag
      241 caaaaggtgt tctaatgtca ttgccgggac agcaatcttc aacggatatg gagaatataa
      301 agggtttccg gctgcgtcgg ttgatattat cggctgaagg ccaaataata cagcagtaat
      361 taatgctgca acgttaaggc ttacataccc ggcaaaaaat gctgcaacac ctgttcttct
      421 tttattatca gtgtttccgg caataagctt gtatgtccat actgcaataa atggcattac
      481 aatagccatt gtaaagcagt tggcccctat tgctgtaatt ccaccatcgc caaatactaa
      541 tgcctggatt atcagggcta ccgaaacagc aatcattgca gcccaaggcc caataaccaa
      601 ccccgctaat gctgcaccaa cagcatggcc tgttgtgcct ccggggatgg gaatattaaa
      661 catcataatc aggaaactga atgcagaagc aaaacccaga aatggaataa gcttcaatga
      721 caactttttc ttgagttttt tgattgcaac aaaaataaaa gcagtaaaaa tggcatacat
      781 ggtgatgtat gtctttggtc ctaagtatcc gtcaggtatg tgcataattt atttaatttt
      841 taaaggacaa catttagcct caaataagca gcaaaagcat tatctaatct ggctataact
      901 cccggattta ttacataatg tatggttggt tgcaatgaga ggtgcttgca aacatatata
      961 tggtagttta actccaggtc ggtttcaatt tttttatcaa tgcgtgcaag agcaataccc
     1021 aaagataagg catcttgaat acattttgga caaatgccgc tataacttac gccaaatccg
     1081 gcataccaat cgttgatgtt aattttgtca ggagcatagc ttatttgcat aaatgcacct
     1141 aatgtttttt cattgttttt atatattgtt tggtctataa tgccataaaa gccataattt
     1201 cctttaatta ccgaatctgt gcggcttgtg ttataaaatt ttccagaatg ataatagcta
     1261 cctattttta atacctgatt aatagaagga atattgcatt cgagctcata aatagtaagc
     1321 aatccctggt ttgcactaat tgaccacgat agattgtatt tttcgtcgtc caatgaacca
     1381 gcatcgccat cgtagaagcc taagcggtgt ctgatgtttt cattttgctt ataacatccg
     1441 gccagagcca atgacgtttt ggggtacatt gaaaccggaa aattgagtga aaatgtacta
     1501 aacattccaa aactactgtt tgtaagtgat gccccatact cactggtaca aaattctgaa
     1561 ttcaaatcgt gtattccact gaaaatataa ccattttcaa aagattgtct gtaataagcc
     1621 tggtacaaat atgtgtaatt gccattttca atatttgaaa ctaactgggc atctccaatg
     1681 taattctctg aaagattttc accatgtcca ttttgaagtt ctattgtaaa ttccccgttt
     1741 ttccaatatt tatcggtgtg caaaataaca ccggcatcta tcaatcctat ataagcattt
     1801 ccttttttta tcccgcccga aaagtttctg gctgcatctg cagtatatga aaagataggc
     1861 tcaatttgtt ttttgttgtt gctttcttct tgcgataccg cgttattgct tttcaaaata
     1921 atagctaaga ccaataaaat cgcttcaaat tttttcattt aaaagtgtta cttttttaaa
     1981 attcgtgtta caaatgtata atatttttaa tacaaactaa taattgtctt gaaatgttca
     2041 aaaattttgt tcgtttcaat tttttattga aatacagtaa tgggttgaaa caaattctaa
     2101 atgcaaggtc aaattattca tatataggtt tgtaatattc atatcattcg ttaatttcgc
     2161 aaagtttttc taattacatt acagcaatag aattaaatga gaaaatggcg tattgaggat
     2221 tccgcagaat tgtataatat caatggatgg ggaattaatt atttttcaat caacgaacat
     2281 ggtaacgttc aagtaacccc aaaaaagaat ggggtagccg ttgatttaag ggtactggta
     2341 gatgaacttt tgctcaggga tgttgctaca cctgtacttg tgaggtttcc tgatattttg
     2401 gataaccgca tcgaaaaaat gtcaaattgt tttcgaatag cgtcagaaga gtatggttat
     2461 aaaggccaga actttctgat ttatcctatt aaggtaaacc agatgcgccc tgtggttgaa
     2521 gagattgtaa accacgggaa aaagtttaat attggtttgg aagccggttc aaaacctgag
     2581 cttcatgccg ttattgctat taatacagat aatgattcgc taatcatctg taatggttac
     2641 aaagatgagg attatattga gcttgccctg ttagctcaaa aaatggggcg ccgcattttt
     2701 ttggtggttg aaaaactcaa tgaacttaag cttattgcta atatctcgcg tcgcctgaaa
     2761 atcaggccaa acattggtgt ccgtgtcaaa ctggccagta ccggcagtgg aaaatgggaa
     2821 gaatcaggcg gcgatggtag taaatttggc cttacctcaa gtgagcttct tgaggcgctt
     2881 gattttcttg aaaaaaacaa gatgaaagac tgtctcaagc ttgtgcattt ccatattggc
     2941 agtcaggtga ccaaaatccg ccggataaaa attgccttac gtgaagcttc tcaattttat
     3001 gtccaactct ataaagcagg tttcaacatt gaatttgttg atattggtgg tggattagga
     3061 gtagattacg atgggactcg ttcatcatac agcgaaagta gtgtcaatta cagcattcaa
     3121 gagtatgtca acgactctat ttcaactctt gttgatgtta gcgataaaaa tggaattcca
     3181 catcctaata ttattaccga atcaggtcgt tcgcttacag cacaccattc agttttattg
     3241 tttgaagtgc ttgaaacaac cagtttgccc acctggcctg aagatgaaga ggttcaacct
     3301 acagaccatg agcatgttca agaattgtac aagctttggg acaacctgaa ccagacccgc
     3361 atgctcgaaa cctggcacga tgcccaacaa atacgcgaag aagcgcttga tttgttcagc
     3421 cttggtttga ttgacctcaa aacccgagca caggttgaaa agttgttttg gtcaattgct
     3481 aaagaaatca atgctttatc cactgacaca aagcacgctc ctgacgagct gagacaattg
     3541 ccaaagcttt tgtctgataa gtatttctgt aatttctcat tgtttcagtc tttgcctgac
     3601 tcatgggcta tagaccaggt atttcctatt atccctattc aaaggcttga cgaaaggcct
     3661 gaccgttcag caactttgca agacattact tgtgactcgg atggaaaaat agacaatttc
     3721 atcaccagca aaagtttttc gtattatctt cctgtacatt cgttacgtaa caaagattca
     3781 tattatatag gtgtttttct tgtaggggca taccaggaaa ttcttggcga cctacataat
     3841 ctttttggcg ataccaatgc agttcatgtt actgtaaatg aagatagtta tgaaataaat
     3901 caaattatag atggtgaatc agtagctgaa gtgttggatt atgttcagta caatcctaaa
     3961 aaaatggtcc gtactgttga aacatgggtt acatcatctg taaagtctgg cattattagt
     4021 gctgaagaag gaaaagaatt tctttccaac taccgatctg gtctttacgg atacacctat
     4081 cttgagtagt ttattttttg aaaataagat ttggattaat tgttgtattc caattaaatt
     4141 tgcgcttttt aaaaaaaaca tgataaaaat tacatttccg gataattcag taagagaata
     4201 cccgaagggg agttcagcgc tggatattgc acgttccatt agtagcaatt tggctgccga
     4261 agtgctttca gctacagtga atggagaatt aatagatgca tcaagggcaa tcaatacaga
     4321 tgcatcaata aagtttcaca aatgggatga tgagcaaggt aagcatgcat tttggcattc
     4381 atcggcccac ttaatggctg aagctcttga agccctttat cctggtatta aatttggtat
     4441 tgggcctgct attgagcaag gtttttatta tgatgttgag cttcccaacg gacaaaatat
     4501 taccgatgct gaccttccga aagttgagca aaagatgctt gagctggcac gtcagaaaag
     4561 cgtttatcaa cgtgaagaga taagcaaaag caaagcgctt gaacttttca accaaaaagg
     4621 agacccttat aaagttgagc ttattacaga gcttgaagat ggtactataa cgctatatcg
     4681 ccagggcaat tttaccgatt tgtgccgcgg tcctcacctt ccggatacca gctttatcaa
     4741 agctgtgaaa cttacttcta ttgcaggcgc ttattggaga ggaaacgaaa aaaacaagca
     4801 acttacccgt atttatggta ttacctttcc aaaacaaaag ttattggaag aatatcttgt
     4861 aatgcttgag caggctaaac tccgcgatca ccgcaagatt ggcaaagagc ttgaactatt
     4921 tgcttttagc gaaaaagttg gtatgggctt accattatgg ttgccccggg gagcagcatt
     4981 acgcgaccgg cttgaacaat ttcttcgtaa aatccagaaa cagcacggtt atcaacaggt
     5041 aattactcca catatcggac aaaaggaatt gtatgtaacc tcgggccatt acgctaagta
     5101 tggaaaagat tcatttcaac caatacatac tcctgctgaa ggtgaagagt ttttgttgaa
     5161 acctatgaac tgtcctcatc actgtgaaat ttataaaact aaaccccgct catacaaaga
     5221 tcttccaata cgttttgcag agtttggtac tgtttatcgc tatgagcaaa gtggtgagct
     5281 tcatggattg acccgtgtac gaggctttac acaagatgat gcacaccttt tctgtcgtcc
     5341 tgaccagatt aaagaagaat tttgcaaagt aattgatatt attttataca tttttaaaat
     5401 acttgatttt aaggattata ccgctcaaat atcgcttcga gacaaagaaa acagagacaa
     5461 atacattggg tctgatgaaa actgggacaa agcagagcaa gctattatag aagcttcagc
     5521 cgaaaaaggt ttgaatactg ttatcgaata tggtgaagct gcattttatg gcccaaagct
     5581 cgacttcatg gtacgtgatg ctattggccg caaatggcaa ttgggtacta ttcaggttga
     5641 ttacaacttg ccagagcgtt tcgagctcga atacattggc tcagacgacc aacgtcaccg
     5701 ccctgttatg attcacaggg caccatttgg ttcaatggag cggtttgttg ctgttttaat
     5761 agagcacact gccgggaaat tcccattatg gttgacacca gaccaggttg tggtacttcc
     5821 tatcagcgaa aaatttcagg attacgcaga aaaagtttta aattatttaa ataattccga
     5881 tattcgcacc ttgattgaca cgcgcaatga aaaaataggt aaaaaaataa gggacaatga
     5941 gttacgaaaa ataccttacc tattaattgt gggagaaaaa gaagctgaaa atgaaactgt
     6001 tgccgttcgt aaacaaggcg aaggcgataa aggggtaatg acattagacg aatttgttca
     6061 acatataaaa caagaaatag aaagacaatt atcgtaaact ttattattaa ctaatcaggg
     6121 agggtaaaac aatagtagga aaatctaata ctccggggcc taggccagtc caacaaaaag
     6181 ttgttgaaaa acaacatcgc attaaccaag aaataaaagc aaaagaagtg cgggttgttg
     6241 gagaaaatgt tgaaggacaa aacgtgatgc ctttgcaaga agcattgcgg cttgcagatg
     6301 agcttgagct tgatttggtg gaaatatctc cgcaggcagt accacctgtt tgtaagatta
     6361 tggattatga aaagtttttg tatcagcaga agcggaaaca aaaagaaatg aaagccaagt
     6421 cggtaaaagt tgttgtcaaa gaaatacgtt ttggtcccaa tacagatgaa cacgattaca
     6481 actttaagct caaacatgca cagagttttt tgcaggaagg ggcaaaagta aaagcgtacg
     6541 tattttttaa aggacgttct attctttaca aagatcaagg agagatttta ttgttgcgat
     6601 tggcaaacga ccttgaagaa cttggtaagg ttgagcaact tccaaaactt gaaggaaaac
     6661 gaatgagtat attgataaac ccgaaaggga aaaagtaatt taaattaaaa aaagtagaac
     6721 aatgccaaag atgaaaacta attctggtgc atcaaagcgt ttttcgctca ccggaacagg
     6781 aaaaatcaaa agaaaacatg cttttaaaag gcacattctt accaagaaaa caactaaggc
     6841 taaacgtaac cttacatatt ttggtttagt tagcccggct gatgaaaaga atgttcagct
     6901 tttattatgc atgaaataat taatcattat tttttaacct gaattattag caatacaagt
     6961 tcccggcagg cggggcgcta atcgttcata aaattgtaaa aatatgccac gtagtgtaaa
     7021 ttcagtcgct tcacgcgcaa gaagaaaaaa gattttatcg aggactaaag gtaacttcgg
     7081 tccaagggct aatgtttgga ccgttgccaa aaatacatgg gaaaaaggtc aacaatatgc
     7141 tttcaatgac agacgtaaga aaaaaggtga atttcgtgcc ttatggattc aacgtattgg
     7201 agcagcagta aggccttttg ggttaagcta ttcagaattc atcggattgg tcaacaaaaa
     7261 gggagttgcc ttaaaccgca aagcacttgc tgaccttgcc atgaatcacc cggtagcttt
     7321 tgaagctgtt gtaaatgctg taaaataatt tcaaaggata tactcattca tgaaaaagga
     7381 ggccaaaaag cttccttttt ttatatctac ttgtatattt gtataactaa aattaaaatc
     7441 atgaaacacc catgtttgga aaacacaaac actttagtaa taaaatagcg aacagggggg
     7501 ttccatcatt ccttaaaaat taaattttat tatgatgaaa atagcattaa taggatatgg
     7561 aaaaatgggc aaaatgatag aagaaattgc ccttttgcgc ggtcaccaga tttcgtaccg
     7621 catagacatt gacaatattg aagagcttga tcaattaaat ccttcaaata cagatgtagc
     7681 cattgaattt acaacccctc acagcgccta tggcaatgtg atgaaagttt tgaaaaacaa
     7741 agtaaacgta gtatgtggat caacaggatg gaacgacaaa ctgcctgaag cccgtaaata
     7801 tgcagagtct aataatcttg gttttctttg ggcttccaat tttagtattg gggtaaatat
     7861 attctttaag gtaaatgaat accttgcttc gttaatgaaa aacacggatg gttatacccc
     7921 agaaatgacc gaaattcatc atatccataa aaaagatgca ccaagtggca ctgcaataac
     7981 tcttgcagaa ggtattatca aacatttgcc aactatcaat acatggaaac ttgcccctga
     8041 taatgcatca gatgcagtta ccattgaagc aaaacgcgaa ggcgaagtcc ccgggaccca
     8101 tatcattaaa tatgcctcag atgttgatta tattgaaatt actcactgcg cgcataatag
     8161 aagaggcctt gcacttggag ctgttgttgc agctgaattt ttgaaaggta aaaaagggaa
     8221 cttctctatg acagacgttt tgaattttta accatatacc ttcttggttt caaaacctgg
     8281 aaggtttttt cttgttaaaa aagcttataa tcgcctgatt ttctttaaaa aatattgcgt
     8341 tgtctgtgct ttgtaaaaag atttgttact ttagcgcaac aaacaaaaaa caagtttcat
     8401 ttaatcaatc atttgtatga aatcattcct aaagctgtta aaaaataaat acttcaaatt
     8461 tggagtggca atcacaattt accttttatg ggtgatatgg ttaggcaact attggttttt
     8521 attgggtgcc ccaattgttt ttgatattta tgtaagcaaa aaagttaact ggacaccctg
     8581 gaaaaagcgt agtggaaaaa actccaaact tgtagaatgg attgatgcaa ttatttttgc
     8641 tgttgtagct gtgactatta tcaatatatt catttttcaa aattacaaaa tcccgacagg
     8701 ttctatggaa cgttcgttgc tgattggaga ccatttgtat gttagcaaac tcagctatgg
     8761 gcccaggatt cctaatactc cgcttgcttt tccttttgct caaaatatat tgtttggcac
     8821 tgttccttct ttttctacta ttatccaatg gccttataag cgccttgccg gatttggaaa
     8881 aataaaaaat gacgatattg ttgtttttaa ttatcccgaa ggtgatacgg tagctcttga
     8941 aatgcccagt aaccaaagct attatgacta catcaacatg cgtatagaag aattgaaagg
     9001 ttttgatgaa agaaataacg taaaacgtac tgatgaagag tattatgcgc aagcccgtaa
     9061 agaagtatgg gacagatata ctgttgtatg gcgtccagta gataaaactg acaattatgt
     9121 aaaacgttgt gtggcaattg gaggtgatac tcttcaggtt attgacggag aagtgtttat
     9181 aaatggcaaa ccccagaaag atattgatgg aaaacaattt gtatattatg taaaaaccaa
     9241 cggacaacct ttcaactcaa aagtgttgga agatatggat atttacgata gcgatatcca
     9301 atactctgga gatggtgcat ttatgccttt gacaaagttg aattatgaaa aggttcgtca
     9361 tttgagcaat gtaacaggta ttgagcctat attcagagga aagggcgagt gtgatttacg
     9421 agttttccct cataacaaag cgtttgcatg gaaccaggat aactttgggc cattgtatat
     9481 accaaaacaa ggagattcta tagctcttac ccccaaaaac cttatattgt acaaaagggt
     9541 gatagctcat tatgagaaaa ataagctcga agtaaaagac agtatcgctt atattaatgg
     9601 taaaccttct aaatactata cttttaaaat gaattattat tttatgatgg gtgacaaccg
     9661 tcataattca ctcgattctc gttactgggg gtttgttcct gatgaccata ttattggaag
     9721 tcccgttttt atatggcttt cattaaacca aaataaatca ttcccaggaa atataagatg
     9781 gagcaggatg tttatgctgg ctaaataaaa agaagcaaaa aaagaggctg taaaaaaatt
     9841 gtatatttta tcacactgtt gtccggtaaa ataaaaaata gatacttata aatactagta
     9901 atatcgttga atagaaactg gttgccctgc tggggcataa ttttaacgat gtttaatttt
     9961 actgcaaaca ggacgtggct atgcaactaa ataatagcaa cgtagctgcg aactgttgta
    10021 ggacagtcca atacacccaa aggtctctaa gctgcttagc ggcgatcttt ttttaccaga
    10081 caacagtgtt aaaaatcagg gcttcaatct tttatttatt gggcaatata ttttactgta
    10141 tttgagctgt gttcactttg aacattgatg aaataaactc ctttgctcaa tttattgaga
    10201 ttgatttgtt tgctgaactc ttgtggcgtt ttgtattcat tagattgata tactaaggtc
    10261 ccgtttattc cgaatattct tattttgagc ctttcggtat aatcagattt gtaatgcacc
    10321 gtaaaaaccc cttttccagg attggggaac aatgtaaggt tttctgatgt tgaaatttga
    10381 tttatagctg ttgttatttc agtatcggta atggttatat aaaattcgcc ttcaacgccc
    10441 ccaaaactgc catcaacctg caaccaatag gttttccctg gaattacggt aatttcgtca
    10501 agagcggcag cgtaattgtc ttctatacca taataatcgt cattggcagc aacaaggtct
    10561 tcaaacttta tattgtcgca gctttcagaa gaatacaggg ctaattggtt gtccatgcct
    10621 cttgtattaa cagaaagttt gccattgtca ggaccgataa ctttgaacca tacagaattc
    10681 tgcaccccgc cttcattaca ccaggtttta tctaagtagc agtttgtatc tgcaggatga
    10741 acttcgttat aatccgctgt agccccaacg tttgagaatg ggccattttc tcccgggctt
    10801 aatgttattg cattgcaagg tttatcgttt gatgggggat ttttcaggta aacgttcagc
    10861 gaatattgtc cctgacaatt accttgtttt gctgttaaag taaatttata taaacctgga
    10921 ttgtcagctt taaatacaac cgattttgaa aatgtgtccg ataaattagt tgatgggtac
    10981 cagttgtagc tgtctgcatc aacaatagct gtaagtattg ctgaatcaga ttgtttgatg
    11041 tatgtcgggg ctgcaataag tgttgtaagc gaatttacaa ctttaatagg tttactaaac
    11101 gaaacggtat catttcctcc aataacatat aaagccggat agcgaagccc agatttagcg
    11161 ttgctgctgt atgtcactac gtgcggcccg gctccggtag cataggcagg agaagcgtct
    11221 tctccaaata cccataaata tttattaata tttccgtttg ttttggctgt ataagtaact
    11281 gtactgcctg cacatacttc atatttatct gtagtaaata ctgcaacagg ttttgaaggg
    11341 gtaatagcat acaggtgagc tataatgttg gcttcccatc caacaacatc agaacctaat
    11401 gcctgccaaa tattcccgtt agcgcttacc cattgatagc cggatggata aacaaacgga
    11461 ttggcgtact cttcgccatt gacatcagtt tttaattcaa caggtataga atagtttact
    11521 cccggtgtaa aataggtaag cttaacgtaa aaagtacctt caacttctgc cataatgtca
    11581 aaagcataag tgccggggca ttgcactggc acattaaccg aatgatggat taatgttgac
    11641 agagaatcag agtaaatatc aatagtaagg gatgagcctg ttacaggcaa aaaagtactt
    11701 aagcttttta acagcttttt tgaagcagaa gtgtatttta acaaggcata attactttct
    11761 ttttcatatc caaaaaagct tacaacgcca atattattgt atttaaatac agtgtcaaca
    11821 gattccgtag cccacatagt tggataatac gaagcaggtt ttaatatttg tttatctcca
    11881 taagcgatgt aaaaaaaacc tgaagcgccc catgttgtgc cccagctgtt tttagcaatc
    11941 catgcccctt tgccagcttg ggttgtaata gtatcgttcc atcctacaat actaatggca
    12001 tggttgggcc cggttcggtc tgttgagtaa aaaatgtttg ttttcttttt ataataggca
    12061 gtgtcccaat ggatgttagt tgctactgct ccatagtcca ttataacttt ttttatcatc
    12121 gggatatttc cataaatacg tcgtgattca ggaaagtaag caacaggttt tgctttttta
    12181 caagtaaatg tagatatgtc agtggtattg tatggtacat ctgtttctaa aacggcacct
    12241 tgaagccgcg ataaataggc ttgtgcaaaa aattcgttac cgcctgcccc aaaagcccaa
    12301 ctgtaaccat gacaggttgc catgttttgt tcagataagt caagcgcatt gatagcagaa
    12361 agtggattat accacattga ctcaatagag ctcaaagctg caaaagccca acaattgccg
    12421 cctgtattgc cacttccctg gttttttaca ggtgaaacca ggttgtcata tttttggctt
    12481 gtttcgttat atatttttcg caaatcaaat accgatggaa gccctgaaac catagcagat
    12541 ttaagttttg agctttcttc attttctata taatattcgc tcaattgtgt ataaggaggg
    12601 atatacccgg ttgtcttttc atcaatagca gcccgcaatg attttttctt ttggtaaaac
    12661 tcagtaagtt ctttgttaac agggggtatg tgttttttca attgggcaat tcctacagaa
    12721 aaagaaaaaa acaacaaaag ggtagtaata aatgcctgcc tgaagtttct gatattcatg
    12781 ttataataga tttcgtaatg tttgtagata ttcaatggtt ttaaaaacat aaaattagca
    12841 aaaattatga cataatatac ttggtatgat agttgtcaac ttaaaagatt tttggttcac
    12901 aactttttgt ccaactaaca agtagttgcc ataaaatagt atatttttgt tgccaaaatt
    12961 ttgaaaactg aattttttta agatataaaa acaataaaat gagcattatt gaaaatttga
    13021 tcaggagttt ttttggttct aaatctgacc gtgatattaa agaaattatg ccaattgtgg
    13081 ctaaaatact ggcagaatat gataagctga aaaacctgag caatgacgaa ttaagggctt
    13141 taagctcgaa tttgaaaaac gaaatcaggg acttgataaa aacagaaaac gagaaggttg
    13201 atgccttaaa acaacaagca gacaatcctg agctatcgat agaagaaaaa gaagatattt
    13261 acagggacat tgataaactt gaaaagcaaa tagataccct tgtaaaagac aagctggacg
    13321 aaatgttacc attggcattt gccattgtaa aagatacagc ccgccgtttt tgtgaaaatg
    13381 aaaaacttga agttacagct actgattttg accgtaatct tgcttcaagc cgtaaaagta
    13441 ttgaaattat cggggataag gcttattata gtaaaagatg gatggccgga ggcaatgaaa
    13501 taacatggga tatgatacat tacgactgcc agttgattgg aggtattgta ttgcatcagg
    13561 gtaaaatttc tgagatggct accggagaag gtaaaacatt ggttgcaact ttgcctgtgt
    13621 ttctgaatgc tttggccggg cgaggggttc atgttgttac ggtaaacgat tatcttgccc
    13681 gtcgtgactc ggagtggatg ggtacgctgt acgagtttca tggcctttct gttgattgta
    13741 ttgataagca tcagcctaac tcagaagcca ggcgtaaagc atatttggct gatattactt
    13801 ttggtactaa caatgagttt ggctttgact atttgcgaga caacatgaca agcagcccgt
    13861 ctgaattggt tcagcgtgtc cataattatg ctattgtgga tgaggtggac tctgtactta
    13921 ttgacgatgc ccgtacacca cttattatat ccggcccgat cccaagagga gaaaaccatg
    13981 aatttgaaat gttcaaacct aaggtagagt cgttgtataa cgctcagcgt aagcttgtta
    14041 cacaaatttt ggcagatgca aaaaaagcta ttgatgccgg cgacaaagaa aaaggtgggt
    14101 tattgttgtt gagggcatac aaaggcttgc ccaaaaataa agctttaatc aagttactta
    14161 gtgaagaagg cttgaaaaac ctgatgctta agaccgaaaa tttttatatg caggacaata
    14221 acaagcaaat gcaccttgtt acagatgagc tttattttgt tattgacgaa aaaaacaata
    14281 gtattgagtt gactgataaa ggtattgacc tgattacaac cacctcagaa gactcatcgt
    14341 tttttatcct gcctgacatt gggagtgaaa tagcggatat tgaaaaaaat attacagatc
    14401 cggctaaaaa acttgagaaa aaagaaaaac ttttgcagga ttatgctata aaagccgaac
    14461 gcgtacatac cgtaaaccaa cttttaaagg cttatacttt atttgaaaaa gacattgaat
    14521 acgtggttat ggacaacaaa gtaaaaattg ttgacgaaca aaccgggcgt attcttgaag
    14581 gccgccgtta ttctgatggg ttacaccagg ctattgaagc taaagaaaat gtaaaaatag
    14641 aagctgcaac acaaaccttt gcaaccatca ctttacagaa ctatttcagg atgtacaaga
    14701 aacttgcagg tatgaccggt acagccgaaa ctgaagctgg agagttttgg aacatttaca
    14761 aacttgatgt tgtggttatc ccaaccaatc gtccggttat tagaaaagat tttgaagata
    14821 aagtatataa gacaaaacgc gaaaaatata atgccgtaat tgatgaaata gctgagctta
    14881 tcgaagctgg ccgtccggta ttggttggta ccacttcggt tgaaatatca gagttgctta
    14941 gccgtatgct caaaattcgc ggtattaagc ataatgtact aaatgcaaag ttgcatcaaa
    15001 aggaagcaga aattgttgca gaagctggta aatcaaagac tgtgactatt gctacaaaca
    15061 tggcaggtcg tggtactgac attaaactat ctgctgaagt taaggctgcc ggaggtttgg
    15121 ccattattgg caccgaaagg catgagtctc gccgtgttga cagacagttg cgtggtcgtg
    15181 caggccgtca gggcgaccct ggttcttcac agttttttgt ttcgttagaa gatgacttga
    15241 tgcgactctt tggttctgat cgtattgctt ctatcatgga caggctgggg ttgaaagaag
    15301 gtgaagttat tcagcattca atgatttcaa ggtctatcga aagggcacaa aagaaagtag
    15361 aagaaaacaa ctttggtatc cgtaagcggt tgctcgaata tgacgatgtg atgaactcgc
    15421 agcgtgaagt gatatacaaa cgtcgcagaa atgctttgtt tggagagcgt ttgaccctcg
    15481 atatatccaa catgttgctt gatatatgcg aaaatattgt tgaacgcaat catgaggctg
    15541 gagactttga acagtttaac ctggatataa tcatgaattt agcagtagaa tctcctttta
    15601 ccgaaaaaga gtttaaggaa atgtctgtta aagaaatgat tgaaaaatta catgctggtg
    15661 tttgggaagc ttataagcat aagatgaacc agatagccca acaagtggcc cctgtaatta
    15721 aagatgttta tgagaaacaa ggggcacagt atgagaatat ggttgttcct atctcagacg
    15781 gcatacacgt atatcagatt ttgttcaacc tcaagaaagg agctgaaagc aacggggctg
    15841 aaatgataaa aagttttgaa aagcttgcaa ttctgatgaa tattgacgat gcttggcgtg
    15901 agcaccttcg tgaaatggac gacctgaaac aatctgttca gaatgctgct tatgagcaga
    15961 aagacccgct tttgatttac aagtttgagt catttaagct tttccgcacc atgattgaga
    16021 atgtaaacaa gcaaatagtt tcattgctaa tgaaagggca tgttcattct ccagaacaaa
    16081 atcaaccgca gcgccaacaa gctcctcaca aaattgattt gagcaaactc aacatgagcc
    16141 gttcaaattc attgccggga agcaggcccc agatgaatac caacagagaa caacaaccta
    16201 tgcagcctat taaggtagaa aaaaggattg gccgtaatga tccctgccct tgtggaagcg
    16261 gtaaaaaata taaaaactgt catggacaga acgcagaata attaacaatt aaaattgaaa
    16321 aatatggcta ataaaagaac tctcaagaaa gatattaatt atctgacata cgaactgatt
    16381 tcagaagttt tggtttacag attttatcat ccagaggctg atcaaaatgt gttgaacgaa
    16441 attttgggaa cactgatcga aaaccgaaat aaccttgttg gcaggattaa ccatatcaac
    16501 gggaaagaca accccaagtt ggtgaaacag cattttagct ctatccgtaa ggattttcaa
    16561 aacagcgtga ctatactcga taagttggct aaaactaaaa agtaataatt taaggctatt
    16621 tagttttcta tgtttgaaag gattagggca aaagttctta aagaagacaa tcttgttcag
    16681 tttagaaaag accattccga caaaaagata gtgttttgta ccggttgtta tgacatattg
    16741 caatctgggc atgcggtgtt tttcaatcaa tgcaaagagt atggagacat tcttgttgtc
    16801 ggggttggtc gtgatgctgt aattacaaag ttaaaaggag ctgggcgtcc tgtaaatcct
    16861 gaaaataaca ggttgtatct tgtagcatct ttgcttgatg tggattacgc cgttttaaat
    16921 ggcgaagaaa tcaggcctgg caagatagac ttttataaca ttattgaaaa gctccgtcct
    16981 gacgtatttg tcctcaatga tgatgactct gcattagcag aaaaggaggt actttgcaat
    17041 aagtttggag tgaaaattga attggtgtcg cgcgttgttc ctgaagaatt ggaagcaaca
    17101 tctactacac ggattataga taaaataaac ttttctttta aagctccttt gcgtattgat
    17161 tttgcaggag gttgggcaga tgtaccttat attatgggcg atgaaactgg ttatgtaacc
    17221 aatgttgcca tcaaaccgct tatagaatat aataagggaa agtttaactt ttctggttac
    17281 ccgcgtggaa gcgggctaag tacatctacg gcagtgaaat tgcttgaaat gattagtgcc
    17341 aagaactaca attcagactc aaagcctcta aatgttatag cagaagacct ttttaacctt
    17401 gaaaatacag agttgaagtg ggctataggc cgtcaagacc aatatgccat tgtttatggc
    17461 gggttcaatt gctttgaatt tggcaaaaac tatggaaaac ctgttggtac agaaacagat
    17521 aaagaaaaac ttgagaaatt cagggccaac cttttgcttt tgcataccgg ggtgtctcgc
    17581 aatgctcaat cagcagttga acaggtttat cttaattttc atacaactga aggaaaagct
    17641 gcccttaaga aaataagcga ttgtggaaaa gctttcttca ctgcatttca acaaggagac
    17701 tttatgaaat gtgcttcaat tatggaagaa aactttgaag cccaaaaaca gcttgccaaa
    17761 gctacatcta acgaatacct tgatgaaatg tatgcttttg ctaagcaaaa tggagcctat
    17821 ggtggcaaaa tatgcggagc aggcggtggc ggggctttca tattttactg caaagacccg
    17881 gtgaagctca aacaagctat gaaacatcag tttgtggatt gtttcgagat tgattttgag
    17941 ttcgaataca agagtataaa ggagttgaat actatttagt gtttgatggt gctgattatt
    18001 agagggataa aattgtttat atttttttat ccagaaaatc cgcaatcctt gaattaataa
    18061 tcctttcgtt aacaattatt atttttttta aatggtcaaa ttttatattt ttgattgttt
    18121 gaaaaagaca gaaaatacaa ttagtaacta atttaaaata aacgacaaga tgaaatttgt
    18181 aatttccagc aacgtattat ttgagcgttt acaggctatt aataaagtaa ttagcagcaa
    18241 aaacacttta ccaatccttg acaatttttt gtttgacttg tctgataaaa aattgaccat
    18301 aacagcatca gatttggagt ccactatgat tacttccctg gatcttgaaa acgctgaagg
    18361 agaaggggta atagctattc ctgccaagcg tttgatagat acattgaagg aattttcggt
    18421 gcagccgctt acttttgatg tcaatacaga cacttatgct actgtaatcc ttacccagaa
    18481 cggtaagttt accattaccg gccagaatgg ggatgatttt cctacattag caaggcttaa
    18541 agacgataaa aagtctgtta tggtggatgc caatatatta ttcagtggta tttcaaaaac
    18601 gttgtttgct gcagctgaag atgacctcag gcctattatg aatggtattt ttatggaaat
    18661 ctattcaagt gggttgacat ttgtggcttc tgacggtcat aaactggttc gttaccgtcg
    18721 cacagatgtt accgttgaag aagaaggttc atttgtgttg ccccaaaaac cagctacttt
    18781 gttgaagact gttttggcta aagagcaagg aactgtggct gtttcttatg atgatagaaa
    18841 tgcatatttc caaatggcca actataaaat ggtttgtcgc ttgctcgaag ggtcataccc
    18901 aagttatgct gcagttatcc ccaataacaa tcctaacaag atgattattg acagggttga
    18961 gatttatacg accatcaaaa gggtggcagt gttttcaaac caggcaagta acctcatcaa
    19021 gttacaaata gccaacaacc agttgattgt ttctgcccag gatatcgatt tttcaatttc
    19081 agcattcgaa aggcttaatt gtcaatatga tggagatgaa attgagattg ggttcaaatc
    19141 acaggctttg cttgatattt tgtcaaacct ttcgagcgaa catgtggtaa ttgagctttc
    19201 agaccctggt agggcaggta tcatccttcc tgttgagaaa gacattgagg cagaagatat
    19261 tttaatgctc caaatgcctt tacagattag ctaaatttta gtttatattt gaacatttat
    19321 ttgataaaga cctgtgcgat gcctcggcag gtctttattg cttctaaaaa aaacaaaaaa
    19381 tggaattgaa ccttaaaaat cctttgatat tccttgatct tgaaactaca gggttgaata
    19441 tcgtctctga cagaatagta gaaataacca ttttgaaagt atttccggga ggaaaagagg
    19501 aggtaaaaac ttaccgcgtc aaccctgaga tgcctattga tccaaaagca acagcagttc
    19561 atggcattac agacgaagat gtaaaagatt gccccacatt taaagagctt gcccaaacat
    19621 tggtgaatga atttgaaggc tgtgattttg ccgggtttaa ttctaataaa tttgacttcc
    19681 ctatgttggc cgaggaattt ctgagggcta atgttgattt tgatttaaaa aagcgcaaat
    19741 ttgttgatgt tcaggtgata ttccataaaa tggaacaacg tacgctgagc gctgcttata
    19801 agctttattg caacaaagaa ttagtagatg cacatagctc aaaggctgat acttatgcta
    19861 cttatgaagt tttacaagcc caacttgaca aatatcaagg aaagaaattt gttgatgctg
    19921 ccggaaacac ctctgagcct attactaatg atattgattt cctttcaaaa ttttctgcac
    19981 acaattcgaa cgttgacttt attggccgga ttatctataa tgaaaagggt gtagaggttt
    20041 tcaactttgg taaatacaag ggaatgcctg tggttgatgt gttgaaaaaa gacccttcgt
    20101 attattcatg gatgatgaac ggcgattttc ctcaatatac aaagaatgtg cttacaaaaa
    20161 taaaactgcg cgagtttcag aaatagaaaa agctatggtt gcccagcatc aaattgtatt
    20221 acctgagttc cggagagggt ttcatttagt tacctctcat atagaacgta acttacctgt
    20281 gcttcctaaa actggtatag tcaatctttt tattcagcat acttctgctg cattaactat
    20341 taatgaaaat gccgatcctt ctgtaagagc tgatttcagg acatttacag ataagattat
    20401 tcctgacggc gaccgttgct tttcgcatac ccttgaaggc gatgatgata tgccggcaca
    20461 catcaaatct tctgtttatg gacaatcgtt aacaatacct gtaactaatg gccgtttaaa
    20521 ccttggaaca tggcaaggta tttatttatg cgaatttcgc gattatggag gccctcgcca
    20581 cattgtaata actgtaatag gagagtgata tgcgggaaga tagttttaac tcattgactc
    20641 caaaaaaagt ttggcatttt tttagtgaaa tatgtcaaat accaaggcct tcaaaacatg
    20701 aaaaggctat aatagcttat ttggaaagtt ttgcaaaaca acgaggcttg gattacctgg
    20761 tagataaagg gggaaacata attatctata aaaaagccac actgggttat gaaaacaaac
    20821 ctgttattgt tttacagtcc catgttgaca tggtttgcga aaaaaatgct gatacaaccc
    20881 acgatttttt tagcgagcca ataaatgctt atgtagaaga cgggtgggta aaagctcaag
    20941 gtacaaccct tggcgctgat aatggcattg gcgtagctat gcagcttgca ttgcttgatg
    21001 atattgatat tcagcatgct gcgcttgaat gcatttttac tgtcgatgaa gaaactggcc
    21061 tgacaggggc ttctactctt gaagcttctg caataaatgg ccgcacgatg ataaacctcg
    21121 attcggaaga tgaaggcatc attttcattg gctgtgccgg gggagttgaa acgattggtt
    21181 attgcacgcc tgaatattca aatgttccag acaactgtgc tgcttttgtc cttactgtgt
    21241 ctggtttgca gggaggacac tccggagatg acatcaataa gggcaggggc aatgctataa
    21301 agattcttac ccgcttgctg tatcaatttc agaaacacgc gtcattctat atgtctgata
    21361 tttcgggtgg aaatttgcat aatgccattc ctcgcgaagc taagtcttca tttgtgattg
    21421 aaaaatcaaa aactaccgtt tttgttaaag aaacaaatga tttaatcaaa cacatcctgg
    21481 atgagaataa agacaatgag cccaatcttc aaatccaggt aaaggaaacc ctgcttcctg
    21541 aaaaagtcta taccaaggat ttttcggcac gtttgatttg tacactctat gctttgcctc
    21601 atggggttgt ggctatgagc aatactatcc ctggattggt cgaaacttct accaacctgg
    21661 catttgtccg tcatacaact gatggccgga tagagattgg caccagtcag cgtagcgctt
    21721 tgcaaaccgc taaatatgat atttcgcagc aggtagcttc ggttttcatt cttggaagta
    21781 tggaatatgt tcatactatg ggctatccag ggtggaagcc taacaaaaac tcgcggatat
    21841 taaagcatgc agagggtgtt tatcaaaaaa tgtttggtaa agaacctgtc attactgcca
    21901 ttcatgcagg ccttgagtgc gctttgtttg tagataaaat ccctggcttg gatgttattt
    21961 ctgtagggcc taccattaaa ggggcacact ctcctgacga aaggctcagt attgaaagtg
    22021 ttgaaaagtt ttggaagtat ttgattgaaa tacttgaaac gtggtaaaat ataaataaaa
    22081 gaatcttatc atctgcaatg caaaggaaat accagcacct gttttttgat ttagaccgta
    22141 ctttatggga ttatgatgct aatgcaaaag aaacgctttg tgatatctac gaaaaatatg
    22201 accttgcttc taaaggggtt gatctggata aatttttgca tgattttacc cgctttaaca
    22261 acatcctttg ggcccagttc cgtcatggtg aaataaaaaa agaatatctc aaggttcgca
    22321 ggttttatct tactttgaaa agtagccaga ttaaagataa tgatttggca aaggctgcat
    22381 ctgatgatta tctttcaatt agccctgaga aaaccagatt aatacccggg gctatggaac
    22441 tactcgaata tcttagtgga aaatatcaca tgcatattat aaccaacggt tttactgagg
    22501 ttcaatttaa aaaattgaga aattgcaaac ttgaaaagta tttcgaaagg attgtaactt
    22561 cagatatggt aaaggccaat aaacctaaga aagagatatt tcattatgct ctgtctggcg
    22621 ctaatgctca aaagtcaacc tcattgatga ttggtgacga ttgggatata gacattatgg
    22681 gagccaaata ttttggtttt gaccaggttt atttcaaccg tgacggaaaa gagctgaaag
    22741 gaaaagctac ttatgaaata cattcattag aagaattaat aactatactt acataattca
    22801 aacaaaaacc aatcaactat gaaaaaaaaa tttttttacc tcgtattgtt tagtttgcct
    22861 attttaggct gtgcgcagac taagctaatc ccttttaaca gtagtaatat ctattatgaa
    22921 ggcaggatgt tgtataaacc agaagctgct gtttttaact ggtccggcaa ctctgcaacc
    22981 attataattg atggtacaga gatttctgca gtaatgaaag acttagatac tgccaactat
    23041 tacaatgtta ttgttgatga tagcgtaata gctaagtttc ataccgatac agtaaaaaaa
    23101 agctatgtat tggttagtgg tttaaaagcc ggtaagcaca aagtacaaat ttttaaacga
    23161 acagaatggg ataaaggtaa aactttgttt tatggctttg aaactaattc aaatacaaaa
    23221 gtgttgcctc cattgcctcc taagaaacgc aagattgaat tttatggcaa ttccatcacc
    23281 tgcggatatg ccaatgagga ctttaccaat caagaccgtt ggtttggcta ttttcagaat
    23341 aattatctta cttatgccgc tatgacagcc cgtcatttcg atgctcagta ttcatgtgtt
    23401 ggcaaaagtg gcattggtat tgtcatcagc tggtttccac tgttgatgcc cgaaatgtac
    23461 aatcgtcttg acccaaccga ctctacctct gtctgggatt ttagcaaata cactcctgat
    23521 attgtggtta tcaacctctt gcaaaacgat tcgtggttga ccaatcgccc tgaccatgag
    23581 cagttcaaat acaggtttgg gacaaagaag ccagaagtgt cttttgttat tgaggcgtat
    23641 aaaaactttg tatcgtctat tcgtaaggtt tatcccaatg cccatattat ttgtgcttta
    23701 ggaagtatgg atgccacacg cgaaggttct ccctggcctg gatatatcga aactgctgtc
    23761 aaacaacttg ctgacccgaa agtatataca tgtttctttc cttacaaaaa tacaggcggg
    23821 catcctaaag tgtctgaaca taaagtgatg gcaaaccaac ttattgactt tattgacaag
    23881 aatattaaat ggtaattttt ataaaagcct ttttgaatat aattcttaaa ggcttttttc
    23941 cttgaataga aaaatgtttt ttaatatttt aagataaaat tcaacacatc aacgtcttga
    24001 ctcttgcttc atcaattccg gccctttcaa tttgttgcaa aataatgaat aggaaagacg
    24061 gtttaccttt ttttaaaata ataaaatata tatatttgca gccttgatgt taaaaacggt
    24121 ctggtagctc agttggatag agcaacagcc ttctaagctg tgggtcacag gttcgattcc
    24181 tgtccagatc actaaaaatt taaaacctaa caatatgtct gaagtaaata acgagcattc
    24241 aggaattgag aacgtaggcg aagcactaag caaagccgaa ttgtttattg aaaaaaataa
    24301 aaagccgatt atcattgcag ttgcagctat tgttttagtg attggaggct attttgtttt
    24361 caaaaaagct tatgtagctc caatggaaaa agaagcacag gaacaaatgt ttgttgctga
    24421 gaaatatttc gaaaaagact ctttccagct tgctcttaat ggagatggta acaattgggg
    24481 ttttttaaaa attactgaag aatacggaat tacaaaagca gctgaccttg cacattatta
    24541 tgctggtatt tgttatttac aaactggcaa tttcaaggct gctattgaac accttgaaga
    24601 gtatgatggc gatgacaaac ttatagcccc tattgctgtt ggtgcaactg gcgatgctta
    24661 tgtagagctt ggcgatttaa agaaaggagc taaactttat gagaaagctg ctgatatgtc
    24721 tgaaaacgac tttacctcac cactttattt aatgaaagct ggcgttatat atgaaaatct
    24781 taaagacttc aaaaaagctt tagcaattta caaacagatt aagaaagatt ttcctcgtag
    24841 tactgaaggc cgtactattg acaagtatat taccagggct gaattactgt caaaataatt
    24901 tttgagccaa aagatggcaa caatttacaa acctgcatac aatgaagtcc cttcgggggc
    24961 aggaaagtca tttggtattg ttgtaagcga atggaaccct gaaattacag aggcattatt
    25021 agatgctgct tgtagtacac tcctgaaata tggggtaaaa gaaaaagaca ttcatgtaaa
    25081 aagggttcca ggcagctttg aattaacttt gggtgcccag ttttttgccg aatatactga
    25141 agttgatggt gtaatttgcc tggggtgtgt tattcagggt gatacacctc atttcactta
    25201 tgtatgtcaa ggcgttacac atggaattac agaattaaac ctcgaataca acattccatt
    25261 tgtttttggg gtaatcacta caaatacttt tgagcaggcc aaagaccgtg ctggcggaaa
    25321 acttggaaac aaaggggaag agtcggctat cgttgccctg aaaatggctg cccttcagga
    25381 tgaaatgagc aaataagttt attcgggaaa atcatccact tcaatcaggg caaaaggtat
    25441 ttttacaatt tctttgaagt cagtacctaa atcaagcatt tccttatcat ctttgatgta
    25501 gtaccaattt acggtaacac tcaggttgtt ttggaacaaa tacatgttca ggtaatcaaa
    25561 taaagacatt aaaaccctgt tagtgctgct atttgaatag tcgagcatga tagatacttc
    25621 ggtgtgcttt tcgggatgtt tggcataatt ggccacggct tcaaaaagcg ggtcatacgt
    25681 ttcggtaata tcttcgggaa tagacctgcc agctatcagg agtttgcctt tgtcaaattt
    25741 gattaaaggg gtatgggcgg ttctttctaa ataaaattcc attgtcgtat gattcaatta
    25801 tcgcacactt ttcattaagt aaaattaata tcttttttga aatagagaaa ttttagaccc
    25861 ctaaaattat aaatatcaat aaattgacgt tatttgtcat gcccaatatt aattatccgc
    25921 aaattaccat gttcactttt acatcgtgag gaagtgccgg aatactttca aaaagttgaa
    25981 actcaaagca tacccctatt ttaaaagcat tagaagtgga caaaaatcgg tcataatagc
    26041 ctttgccgcg tccaagcctg ttgccagact tgtcaaaggc tattccggga ataataaggg
    26101 cttcgatttg ttcaatatca ctgaaaaccg tacctgtcgg ttcgagaatg ttgtaacgat
    26161 tttttttcat ctgactttca ccctcaaact gtgaaaaaac tatgtcatca tcagagacta
    26221 caggcaaaag gatagttttt gatgtggaat attttataat taaaggcgtt aaatcaactt
    26281 ccatcggcat aggccagtac atggcaatgg tttgcgaatg gacaaattgg ggcagtcgtt
    26341 caattttgtc agcaattatt tgcgagtgga ctgggtaata gtcttgactt tgcttctttt
    26401 tcaattcctt gatggaattt cttatttctg atttattcaa catatacagt gtcaggtatt
    26461 gctttatttg ttaacaaaag gcctgaataa gtaaaaataa tgttattttg taaactggta
    26521 tatggttgtt cgaaaattgc attaaaattg ggtaaaaaag cgtgaaaata aaaatgtatg
    26581 tctaagcttc tgaaaattaa tgcttctgcc ggttcgggca aaacctttcg tcttacccgc
    26641 gaaatgcttc ttttgttgac ccgcaaccct gaaaactaca aacacatact tgctattact
    26701 ttcaccaaca aggctgctgc tgaaatgaag agcagggttt taaaggcatt gcttgaaata
    26761 aatacagaca aaggctctgt gcacctcaaa gctttggctg accttaataa aatgcctgtt
    26821 gaagccattc agaaaaaggc tgatgaagcg ttgttcaaca ttctgcacaa ttattctttg
    26881 ttatctgtaa taaccatcga cagtttcttt cagagggtta tcaagtcgtt tgcccgtgaa
    26941 atgggctttc aaatgaactt tgatattgaa atggacctgg attccgtcct tgtttcttca
    27001 gcccatgaaa tgataaatga cctgaaaaca ggcgataaac taaccgaatg gcttgcagag
    27061 cttgcaataa aacgcattga agaaggtaaa agctggagct ttgaagatga catcgtttcg
    27121 atgggtaaac aattgtttaa cgaagatttt aagctatttt cagaaacctt tcacaataga
    27181 attaaagaca gggattggtt attgagatat aagcaattgc tcgtacagta taaaaagcac
    27241 atagaagtgc atacactgaa aatgttggcc gttttatctg ctgatgccaa aaggataatg
    27301 gataaatacc aggtaggtgc tgattctttt aaagagaaga gcaggggaat aggaacattt
    27361 atgcttagct tggataaata ctttgttaaa gaccttcctg cctgcgttaa taaatataaa
    27421 gatgatgtaa gcctctggcc gcagtccgat aaggtggcta atagtgtaat tgctgctgca
    27481 aatgctggtc ttgaaaagat tttgaaagat gcagtgaacc tgattcaggt aaaaaagaaa
    27541 agttttaaca gttgcagcct ggtgttggac aatttttata cgctgggaat cctttcggaa
    27601 ttgagccaaa agattgacga ttattccaaa gacaataact tgtttctgct gtccaatacc
    27661 gcttattttt tgcatgaaat aatcgggaat aacgatgtgc cgtttgtgta tgaaaaaatg
    27721 gggcagatgt attcgcattt tatgattgac gaatttcagg atacatcaat cattcagtgg
    27781 aatacgctga agccccttat cgctggtagt ttagcatcgg gcggaaagtc cttagttgtg
    27841 ggcgatctga aacagtctat ttatcgctgg cgaaataccg actggcgcat catggcccaa
    27901 aaggttgatg aagacgtgaa agagcaaggg gttaaagaag aaacgctgga taaaaattac
    27961 cggagcgact ataatatagt ggctttcaac aattctttct tttttcaggc tgcggaaagc
    28021 attcagaaat ctttgactga cgagatggta agtgttggtt ttgaagacaa tatcttcaaa
    28081 acaatgatta aaaaggctta tgaaagtaac tttcagcttg ccaaaaatca ggataaggga
    28141 ttggtcaaaa tagatttcat cgatactacg gagaaagaag ttgaaggcga aaaggtaaag
    28201 cttgatttta aagcagaatc gcttcaactc atgcttgcag accttaaatt gattggagag
    28261 aaaggttcga cagcaattct cgtaaggact aataaggaag ggcaagaggt aatggacttc
    28321 attaatgaaa ataaccggct tgaagaagac ccaactaaaa gaatagctgt aatttctgat
    28381 gaaacagcat tgctatctaa ttcagtagca gtgaatttca tacttaacat ttttcagtat
    28441 tatataaacc ctgtaaatgt tataaataaa gaagaactgg tcaataactg gaatattatt
    28501 ttgtctgaaa tgcaggctaa tcaggctaaa gtgcaatgga atgaattgtc tgtattgcta
    28561 aaggaaaatg aacattttcg ttttcttttg cccgacaggc ttttagaaaa ggttattgag
    28621 ctgttccagc taaatcaggt tacttcatat cttccatatc tgcaacgctt ttatgatgta
    28681 gtcaatgagt acctgcaccg atcgggcagc aggcatttgc tgggttttgt tgattattgg
    28741 gaagaaaaaa aaagcaaaac atctttgtct ttgactgaga atcaaaaagc agtgaggata
    28801 ataaccctgc ataaatccaa aggtctgcag tttgacaatg ttttaattcc tttctgcaat
    28861 tggcctatgg aatataaaaa ttttgatgca cctttgaaat gggtacaagg cgattatcat
    28921 ttgtttaaag ccattgaaca tgctccggtt caattctcaa aggccatgga aaactcctat
    28981 ttttctaaag attattacat agaaaaagct cagttttatt tggataattt gaatttgttg
    29041 tatgttgctt ttacccgtcc taaaaacaac ttgtatttat attgtgagtt taacgagtca
    29101 aagtcttata cagtctcgca tttggtgaat gatgtattga gaaactctaa taatattgat
    29161 ggttctattt ttccgcaagt aaaccttcaa aaatattggg ataaagaaaa tatgtcccaa
    29221 tatcaaatag gcagctattc agttccggaa gacaaagatg ctgaaagtat tgaaaatatt
    29281 gaactatata atttgccagt ttcagcaata gacgaacatc tgaagtcaaa gcctttggtg
    29341 tatgattttg cttcgcagca aaaagcttcg aaacggttga gtggaaacac gatgcacaag
    29401 ctttttcaat acatttataa ggcagaagat atagataaag ctattaacaa gttggtagga
    29461 gagggattaa tttcaagtgt tgacagacag atttattcag aaagagtaaa tagccttgta
    29521 atgaaggagc cttataaaga ctggtattgt ggagactgga aagtttatac cgaagctgct
    29581 attgttgttc ccaaacaaca cgagtaccgg cctgaccggg ttatgataaa tgggaaaaag
    29641 gcggttgttg tggattacaa atttggtttt gtccaaaagg atagctatat taaacaggtt
    29701 tcagactata agcaaacgct tttagatatg ggttttgagc atgttgatgc tttcatctgg
    29761 tatgtttcgc tcgataaata cgtgatggca taaaaaaagc tgcacaattt gtacagcttt
    29821 ttgtttattt gtttgcttaa tattaagctt tgttagcgct tccaagcaca tggatagttt
    29881 tatgcatgta caatttcttg agttcgtcac gagccgggcc taagtatttg cgagggtcaa
    29941 attctgcagg ttttgtagca aatacttcac gtacagcagc tgtcatagct aaacggccgt
    30001 ctgagtcaat gttgattttg caaacagctg attttgcagc ttcacgaagt tgttcttctg
    30061 gaataccaat tgaatccttc aaagcaccac catacttgtt gatagtttca acatattctt
    30121 gtggaacaga agatgaaccg tgaagaacga taggaaatcc gggaattctc ttttcaattt
    30181 ctttcaaaat atcaaaacgt aagggaggag ggactaatat gccgttggca ttacgtgtac
    30241 attgtgcagg agtaaatttg aatgctccgt gtgaggtccc aattgaaatt gccaatgaat
    30301 caacacctgt acggtttacg aaatctacaa cctgatcagg ttctgtatag gtatggtgat
    30361 cagcctgtac ctcatcttca ataccagcca aaactccaag ttcgccttct actgttacat
    30421 catatttgtg agcatactca acaacttgtt tggtaagctc aatgtttttt tcataaggaa
    30481 ggtgagaacc atcaatcata actgatgaga aacccatatc gatacagctt ttgcaaagct
    30541 cgaatgaatc cccatggtct aagtgcaata cgattggaat tgcataacct aattctttag
    30601 catattctac agcgccttgt gccatataac ggagcagtgt ctgattggca tatttacggg
    30661 ctccgcttga tacctgcaat ataacaggtg attttgtttc aacacatgca gaaataatag
    30721 cctgcaattg ttccatattg ttgaagttaa aagcaggaat tgcatatcct ccttttacag
    30781 ctttctggaa catttctttg gtgttaacca aacctaattc tttataatgg atcatcgttt
    30841 tatgatttta attaaaattg ccgcaaagat aattaaaatt gtgaaagaca agtattaata
    30901 acgctattta gatgtaattt agaatgcttg aagtgttttt tttggggtta aaattttgaa
    30961 tatcaataat gtatattttt ttcttctttt ttagtaacat ctgtttatta aacggaataa
    31021 atttctttat tttgcagcaa caataaaaat acatttttat ggacgatggc ccggcgaata
    31081 taatgtttaa tacagcctta accgattagg tgtcattgtt gcaccttctt gtttaaggtt
    31141 taaatgaagg tgcttatgca tattgtaaaa gttttcaaaa tccatttttc aagttacatt
    31201 gaagcattaa tattgccttt gcgaaggttt ttgctgttgt attcttaccg atgttactgg
    31261 taacagtttc acaaaccgga tatgaataaa aatagtatta aaacctgcaa aaagaatata
    31321 atatgcttac aatatggaaa caaaaacaca aattaattga gattgataat ttcgaaagaa
    31381 attgctgggt taatgttact aatccaattc ctgaagaaat agaccgcctg attgaagaat
    31441 ttcgtatccc ggaagattat atccgcgata ttcttgatgc tgatgagcgt tcgcgtaccg
    31501 aagctgaagg ccgttggtta atgataatta tccgtattcc ggtatttcgc acagacaatg
    31561 gtattcctta cattacagtg cctttgggta ttcttatatc ccctcattct attgttacca
    31621 tttgtttgta cgagaatgat gttatcagaa gtattctata tcctgttaaa acaaaggcca
    31681 taaatcttga aaataagata aattttgtac ttgagctttt tcagcgttcg tcaagtcagt
    31741 atttgaaata cctcaaagat attaaccttc ggacaaacca aatagaaagg gatttagaaa
    31801 ggtcaactaa aaataaagag cttcaaaacc ttttgaggat ggagaaatgt cttgtttttt
    31861 tcattacctc attgaaatct aatgagatgc tgcttcaaaa gcttcaacgt tcaaggtttg
    31921 cctccagtga agagctagat gaagaccttt tggctgatgt aattatcgaa acgaagcagg
    31981 ctattgagat gtcgcaaata tatagcgata ttcaggcagg gttgatggat gcttttgctt
    32041 cgttgatttc aaacaacctt aacgtggtga tgaagcagct tacctcaatt accattattt
    32101 tgatgatccc tactcttatt gcgagctttt tcgggatgaa cctgcacaac tttattgaag
    32161 attcgcaata tggctttgtt tctgtagttg taggttcttt actgttatca attattggag
    32221 ttttgatatt caggagacga cagttttttt aaaatttaaa gccaactgtt aatagaaatt
    32281 ttcctgaaga tgtttctaat tcagcctttg gtaaagcgct atggttgtac atcaaataat
    32341 tttctgaaac ggtttgcaaa gagtatgtaa aatcgatgta cgtatttcct gtattataac
    32401 caaagccggc agcatagagc atcgtataag cgtctgagtt gagcattccg cttgaatagg
    32461 ggctttgttt ataggcacaa cctcctcgta agtagaattt gttcagtttt gcttcagccc
    32521 ccaacctaaa cgtttgggca atcctcatat tatctttcat ttctgagttt tcagtactaa
    32581 aatagtcggc tccgtaatcg ctattggaga atttaatttt ggtaaaatcc tcaatttcgt
    32641 aatcaaaact gattaagcct ttatcttcaa ataaatagga gattccagct gtatattttg
    32701 ccggagtagt aatatcataa gattctgaca tggctccaag ttcatatcca tattcgtcag
    32761 ttgggacttc aacaaaagtt ttaccattgt caggcgttgc tgtcatacga gtgcttgttt
    32821 cttgggtgat ttttataaaa cttggagttt ggatagataa tcccaaccgt aaagtctcaa
    32881 taggcctgta aataacccca ataccaaggt tccaaccttt aacccaggtg tccaaattgt
    32941 aaccaaagct aaaagaatta tctgtaatca agtcgttaat gtctatctct gtatggttgg
    33001 ttttatattg ataattgcct gaaataatgt tgaaattgaa ccccaaatat aatttatggt
    33061 catagttagt acccattcca aaataatagg ttcctacaga accatttttt tcaagggtat
    33121 gctgttgctt gagtggaata ttgtcgtatg cactatgata tgaagtgctt cctgaatctg
    33181 catcaatcac aattagtctg taagcaagtt gctcaaaata actgtttaaa tcgttgactt
    33241 tactgccatt ggcgaaatta ataaactcat tggccataga tgtgcctgtt gtcaagccag
    33301 atattttata tgaaccacta taattagcca actggttata tcctaaaaca aggtttatac
    33361 attgccatcc agagccattg tcagtattga tgttcatgtt aaccccgata ttatggaggt
    33421 tcattttgta tataaaatca gtattttcct gaccagaaaa agttgagttg gtattaacag
    33481 aagaaaaagc cggggtgaaa ctaaactggc ttgtcctgaa tacgcctatt ccggccgggt
    33541 tctgtccaat agcccctaaa tcgcctccaa gcgaactgta tgcacctgct aatgccgtgc
    33601 tacgggcaga agaaaaccct tgcatatccg aaagaaagac accatcaatg taatatcctg
    33661 acgaaaagct attttgagaa taacttaacg aaaaaacgga acataatgct attagaccta
    33721 ttagcttttt catatctatg taactttagg ttttcaataa taatcaattt tgaaaaaatt
    33781 aacgccttct tccgcctcct gaatttccac ttgatgagcg ggaacttcca gaatttgttg
    33841 gcgcagaata tgagccactg tttgagttat ttgtttcgcg gcgataaaca ggctgactgt
    33901 tcgaatagtt tccagatgaa ctgtttgaag aaggggtgta actttcagaa cgtctgcggg
    33961 gagcctgatt aacatctgta ttgttttcgc tgcttgtgct ttgtcttctg ttgtatgtat
    34021 tgttatctgt ggagtttgaa taccttctgg ttacaacact gttattatta gaagtctgtg
    34081 atggaggcaa tgtataagcc ggagtattac tatatcttcg ggtactcgat tgtgtattgt
    34141 catattgcgt atatcttgta ctgctggttg ctgaacgcct tgatactgct gtattgtttt
    34201 cagcagtggt aattacatcc ctgcggctta tgccattggt gccagagtac tggttagtca
    34261 aataattggc ccctcgcctt gtcccatggt aaaaatcggc tccggagtat cggcctccat
    34321 agtattttgt agatttatcg ttgatatttg caaaacctcc ggctcgcctg cccggtgtat
    34381 agttataatt acctcttggc ctgtaatagt tgtcgctata ataataacca tcataaaaac
    34441 catgataata gccatgataa tatggaaatc cataatatcc gtagtaataa gggtaatacc
    34501 aataaaaccc caatgaccat gaataatagc catagctaaa cccaaaataa gggtatcccc
    34561 agccataatt gtaaaagtaa ggatcgtaat aataatatcc gtaaggataa taataatcaa
    34621 aatagaaact gcggtaacgc cagaacctgt cagggtcttc ctgaacataa taattattta
    34681 catatacatt ggatgagtcg tatgtaatag tggttaagtc aggttgatta gccacttctg
    34741 ctgtgttatt ttttgctttt acattagatt tactataatc tccatcgcgt aaggccctat
    34801 atttttcgta attggtcatt ccggatgtgt caaccgccgg aacagcaact actgcattgg
    34861 atttatcttt ggatggcata tagtataagt catcctgggc aatggtattt aatccaaagc
    34921 caagtaaaat gacagacgca agtatgccgc tgattttaaa ctgaattgtt ttcatagttt
    34981 gctcctttcc gtgttagtct tcatatatat actcaaaaac tatgcctttg ttcattattg
    35041 tagcttattt ttgatgaatc tgcctgtgta agagttgcgt tcgtttatca aattttcagg
    35101 agttccttca aaaacaatat ttccgccatt atcgcctcct tctggcccca agtcaataat
    35161 ccagtcgcca tattttatta cctccgggtt atgctcaata ataattaacg aatgtccatg
    35221 cgaaattagt gcatcaaaag catctaaaag tttttttatg tcgtgaaaat gtaatccagt
    35281 agttggttca tcaaagataa acagggtagg gccttgcagg ctgttttctt tgctcaggta
    35341 acttgccagt ttcacgcgtt ggctttcacc tcctgatagt gttgatgagg actgtccaag
    35401 tttgatgtag cccaaaccaa cattaaaaag tggttgcaat ttttcaacta ttttattctg
    35461 tgtagctcct tttttatcag aaaaaaactg gatagcttct tctactgtca tttcaagcac
    35521 ttcgtagata tttttgccat gatacctgat ttccaggatg tcgtctttaa aacgcatccc
    35581 tttgcaattt tcgcaaacta gcgtaacatc tggcataaac tgcatttcta cttttatatg
    35641 tccatcgccc tggcacactt cgcatcgtcc cccttctaca ttaaaagaaa aatgggtggg
    35701 tgtaaatccg ttatgaacgg cttctggcac actggcgtat aacttacgta tttcgtcata
    35761 agctttcaaa aaggtaaccg ggttggaacg gctcgaacgt ccaatcggat tttggtccac
    35821 atattcaata ccgctgatga ggtgaatatc cccctcgatt ttgttaaaat ttcccggttt
    35881 ggctatttct gcatttaaaa ccctgctcaa tgctgggtac aatatgcctt ttacaaggga
    35941 agatttgcct gagccgctaa caccggtcac aacagtcatt acatgcaaag ggaattttac
    36001 atcaatgcct ttcaggttat tttccctcgc tccggtcagt tttatgaagc tgttccattt
    36061 tctgcgtgaa tggggtactt caattttttc ttttccggtc agataggcgg cagtaaggct
    36121 ttctttgttt ttttcaagct gttggtggtt accttcaaat attactttac ctcccaagcg
    36181 tccggcaaaa ggccccatgt cgatgatatg gtcggcagcc cgcatgattt cctcgtcatg
    36241 ttcgacaacc actacggtat tgccaaggtt tttaaggttt tgcaaaacct ttatcaaaag
    36301 gtctgtatct cgcgaatgta agccaatact gggttcgtca agaatataga gagagcctgt
    36361 aaggcagctt ccaagtgccg ttgcaaggtt gatacgttgc gattcgccgc cagataaggt
    36421 tgaagatgtc ctgttaaggg tcaaataccc taatccaacg tcgaggagaa agttgatgcg
    36481 gttctttatt tctgtcaaga gccttccggc tactttgttt tcgtaatcag tcaactgtat
    36541 ggtgtcaaag aaagctttga gtgctgaaat aggcattaac acaagctctg aaattgattt
    36601 gcctccaact tttacataat gggcttcggg tttgagcctt gagcctaaac aagcagggca
    36661 ggtagttttt cccctaaaac gggcgagcat cacgcggttt tgtattttat agcttgattc
    36721 ttcaaggtat ttaaaaaaat tgttaatgcc ttcgaagtac ttgttgccat tccaaagaac
    36781 atctatttgt tcctttgtaa ggtctgaata agctctgtgt attgggaaat caaattttga
    36841 agccgcattg ataagatctt gtttccattg gccggagctt tcgcctcgcc aacatgccac
    36901 agcatcttca tacactgata aacttttgtc aggtatcacc agctcagggt caatgccaat
    36961 ggttttacca tatccatcgc agacaggaca agccccaagc ggactgttga atgaaaacat
    37021 gtgaacgctg ggttcttcaa aggctacacc atcttcttca aaacgaatgg aaaattcgtt
    37081 ccatttgcgg tcagataagg taaaaacaaa acattgccct ttgcctatta gcagggcaga
    37141 ctgggtcgag tcgccgcaac gctctctaaa ttcttcattg tcagaggtaa tcagacggtc
    37201 aacaatcagg tttatcgtta ggtacttttt tgcatgggca atagcctcga tatcgtcaac
    37261 ctgtttgata tcttcgccta ctaacattct ggaaagccct tcttttttga attccaataa
    37321 ctggccttca atatttttat cttgcagcgg cactttggca ataagcaaaa tcttttggtc
    37381 caaagggagc gacaatataa agtttaccac gtctgttacc gagtgttttc ggacaacgtt
    37441 gcctgttttg ggtgatattg tttgtccaat gcgggcaaag agcaacttga ggtattcata
    37501 tatttctgta gatgtaccaa ctgttgaacg agggtttacc gagtttacct tttgttcaat
    37561 ggcaatagca ggcggaatac catgtataaa atcaacttcg ggtttctgca tccggcctaa
    37621 aaattgacga gcataagccg ataagctctc aacataccgt ctttgtcctt cggcataaag
    37681 cgtatcaaat gcaagcgagg atttgcctga accagaaagc ccggtgataa caacaagctt
    37741 gttgcgtggt atttctacag ttacattttg taaattgttt acccgggtcc ccttgattgt
    37801 aattgattga ttatattgtt tttgcaaatt tgacatagcg taaaaatgaa aaattgtaag
    37861 cgcttcaaag gtaaaaattt tgctgataag aaatttttat ttattttcga ctcctaattt
    37921 aaaatttgaa tgcctatcgg aaaaagttta cgctactaaa acaaatcttg ctaattaaaa
    37981 aaaggaggta gtcgtgaact atactgtttt ttctgatcaa gagttgatca attcgtttat
    38041 tgcaggcaat aatgaagccc ttgaggtttt atttttacgt cacaaggata aagtctatag
    38101 ttatatttat atcacagtgc gaaacaaatc actggcagag gatatttttc aggatacctt
    38161 catcaaagtc ctcaattcat tgaaagaagg caggtataac gaacatggaa agtttttagg
    38221 atgggtactt cgtattgccc ataatcttat cattgaccat tttcgtaagg tgaagcagat
    38281 gagctttgtt tcagacgaca tattatcagg atgttcatcg cataataaaa atcattttga
    38341 taaaaatgtt gaagatgcta tggtaaaaga gcagatatac agcgatgcca aacgtatgat
    38401 tgaatttttg ccggcagaac agcgtgaagt tgtgttgctt cgtatttttg gcgatttgag
    38461 ctttaaagaa atagcagata tgacccaggt tagtttgaac actgcattag gccgaatgag
    38521 atatgcttta attaatttgc gaaaattggc tgagaaacat aaaattattt tagaagaata
    38581 atttaaccta taaatactat tgggtaactt gttgcgttgg tacattataa aacttaaaca
    38641 aaaagatgcc tatgcaacaa acctctacac ttttttttat tgaagacaat accgacgaca
    38701 gccatataga attcatgcat tcgctgttgc ttaattcaga gtttgatggc tttatttcgg
    38761 tggacgaact aacttatcaa gccccgcagc gggtaacaaa ttatgtactt cagctgatac
    38821 gaaacagctg ataataaaaa agccggtttt tgaagccggc ttttttgtcg tttttttaat
    38881 tactgcttga taatcttgtg cgtatagctt ctttcgttat tgctgatttt cagataataa
    38941 atgccatatt tcatcccaag ttcttctgtc gataagctaa ggttgacatc ccctgcactg
    39001 tatttttgtt tagaaatgat tgattttaca aatttcccgg aggcatcgta caaatcaacg
    39061 ctcagttttt gttgagagct caacttgcat gaaatattta tcacttcttc acaaggattg
    39121 ggaaatactt ttgtagtaga aataaactct ggggtttcaa cagcagtagg gcttttcttg
    39181 ctggtcgtaa cattagcaaa taaagctgtt tcggcagtat attcttgcgc ttgggtaaca
    39241 tatttaatga taacaagaac aggatatctt aaatcttcag cataccagcg gtatgtaatt
    39301 tctttaatcg gcgtactgtt agtgtattca atagtcctga cctgcttaac cctcaaaaca
    39361 ttgtcaatag taacatcgtt gggcaagagc agcgttccgg tagcatcacc atatatttcg
    39421 tattgtccgt taatggcaac agaactattg cccgaggttt gagtccctga atagtaacct
    39481 gtaactttat ttccataaga aaaaggaaat ttcagtttta gaaaaggttt gtcatagcgg
    39541 gttacagtat taccacaaac gctaccatat tgcaacatct tatctttact ggtattgaag
    39601 taaaaaaggt tgccaaattc ttccaaaaca aaatttgagt tgtcgatttt tacatcgctg
    39661 gcaatgtttt cgggggcata catgtgactg gtaaggtctt tatcagattt ttcaagtgtt
    39721 gtaaaatccc agataacatt ttctccggca aggccttcat tggcattttt catcaggttg
    39781 aaatggtgcg aatcgccagg cctgaagctg tgtttttctg aggtcagctt tgtctggcca
    39841 aaaaatattg ctgaacttgc cagcaaaaca gaggatagta aaaatatctt tctcataaaa
    39901 ttcagggttt ttatttaata aaatcaaagt taatgagaaa taagctttaa tacaaattgc
    39961 aggtcaaatt taacttaaat atcatattga agaagaaact ggtacaaagt tttcgataag
    40021 ggcttttttc aattgttcag gtgtgtatgg cttgtaaata gcggcattta ttttacttgc
    40081 tttaagttta ttttctaaat tggggtcttc agacgctgac actactatta ttggcgtatt
    40141 ttgattcttc tcagcagatg accttataaa gctggctatt tcccagccat ccaaaacagg
    40201 aatgtttaaa tcgagcaata caacatcaaa gttttcttgc cctaacagcg ttatggctga
    40261 ttgtccgtct gtagcaatgg aataaatcat cccgaatgaa tctaagcatc tttttgcaat
    40321 aagcaggttt aactggttgt cttctacgat taaaaccttt ttaccattaa gaattgctgc
    40381 gtcattcata ttttattgca tcttttttcg ggcacaaatt gtcatataat ttgatgaaat
    40441 gtccgggtaa aggctaaacc agtattgttc gttgtaaatt tccaggttca tttcttcaat
    40501 tgattgtttg gtgtaaatcg agttaaagat atcataaaaa gatgaatgat aattgtcaga
    40561 tataaattgt tttattgttt cagcctgttt gttagagtat attacgtttc cggtacggtc
    40621 aacccgcata aaagcaaccg ggctgtcatc ggtaatgccg gcaagggaca aaagctgtct
    40681 ttcgtaattt ttacgcctcg aaatatcgac cattacagct cgtaacccaa taggcatgtg
    40741 ctctttcatg atgatattga tataaaccat caccggaatc tcatttcctt cgcggtcaac
    40801 gatggtgtat tcaatgtttt cgatatgttc gcttctgagc attttttcaa aattttccaa
    40861 aagcctttgt ttatctactt gtgtaaaaag attgaagaat attttgcctt tgcttaggtc
    40921 gttttcatta aagccaaact ggatttgcat ttgtttgttg gcaaaggtga ttttgccatt
    40981 gatagaagct tcacaaacca tctcgggcag cagttcggct aattctctga accgtcgttc
    41041 gctcattttc aatgactgga tggcatcatg gtttttgaaa gtattttgca gaatggtcga
    41101 aaatgtaatg aagaattgct tgtcactttc attccaggtt gcacaattgt tgtcaacaat
    41161 agcaatgaaa ccactcatac tggttgaaac aaatatgggg tttaacactg tttttaaaaa
    41221 atcatcgggg tgaaaaatgt tcagaatttc gttgtcttga atattggaaa gttgtatctc
    41281 gaccagttct ttattcatta gcttttgatg tatattgctg atcattgagt atggaatagc
    41341 ctgccattcg ttgcgtttgg gattgaggtc tggcgaatac cattcgtgtg tacacgaaaa
    41401 agcttttcca ctgagtatat tttgaaataa aagtacccgg cttgatttta aaaagttgcc
    41461 tgcaatgcgc aatgactcat taattttata gtcgaagttg tcggaagtgt tcaaaataaa
    41521 agaaatctca gatagcaaac tttgtttttc caggttttta cggatttttc gctcattctt
    41581 ttttctttcg tcaatgttgg acaagaacct gatggcatat tccatccctt ctacctcgtg
    41641 aaattcccat ctttcttcca tctcgatttt taccagacct tgtcttttaa acgtgatatc
    41701 gtggttgatt tttttctcat ttttcagggt ctcaaaaaat tttttccatt tattgatatc
    41761 gttcaatgat tcgtctaagt caaatattga taaatgcaac atatctgcca gcatgaaccc
    41821 ataaagctca gatgattctt tgtttgaata aatgattttt ccttctttgt cgataaggat
    41881 aatcccttca ttagattgtg tcaatacctg gtttaaaata tggcttttac tttgttgttg
    41941 tttttgttcg gtaatatcag aggcaacaac atgtatctgg gtaacatctc cgcttgtatc
    42001 ggagagtgat tgtaaaattc cttcaaacca aatgatattg cccttttcat tatttattct
    42061 gaaatgtaca actttatcaa tgttttgttt tatgcttaat ttgtcaagtt cggcagatat
    42121 taattctttg tcatcaggat ggacaatgtc tgcaaagttg ctttttatca gtttttcttt
    42181 gctgcttttt aaggtgttac aggtagagtt ggaaacgtat tttatttttc cctccggaga
    42241 acatatcata acaatatcgt tggttgtctc agtcaatacc ctaaactgct tttcgtcatt
    42301 ggcaattttg tttttgaggt tagccaatga ttggttcctt ttaagcagct gctggttttt
    42361 taaaaaaagt gagtcttgga gagacttttc aatttttata aaataataaa tgaagtaaat
    42421 gattaaaaat gcagggacaa ggctgctgat gtcatacaaa aagcccggaa cagggttgta
    42481 tttgtaaggc acttcaaaaa gcctgtcctg ggcaaacatg ataaaggaac aaaatgccaa
    42541 ctgggcaata gctgtataaa acctatattt tgaatcgagg ataagaaaag catatatgat
    42601 aattaccaaa ggccaaagta tagccggggc tgaagccgaa taaccaaaaa gtacaaagga
    42661 gaagttgaca gaaataagca ataaggtcaa tgtactataa gaaatccacc tgatgttttt
    42721 ggttttaaaa gcaataacga gcattatcag actggttatc aaataaggca tgctgataaa
    42781 cctaaaaatt tcggatgagg agtaatacaa aaataaattt gaaaagattg aaatgacaag
    42841 cagaaaagca ctgaattgca gtattaaccg gtttttaaag aggtcatacc ggttttttac
    42901 cgagaaaaga aaggcaagag agtatttttt cttctttagg aaaaatttca taatttattg
    42961 ttatgatgat tgataaaatt gttattattg atttttaagc cattaaaaaa taatcaaaac
    43021 cgctgttcaa cttcgtttta ttattaaata tactaaaaaa agaaggaata gttttaaaaa
    43081 agaagcaaaa aatacaaaca gtctgaaaag aagttttagt aaaagttcga aactcatatc
    43141 agcgtattta tttaaaaaag gcttattttt atccagaatt tctaaataag actgaaaatg
    43201 aataaaccct atattggatt attttttgtt ttttgttttg tctcaataca gatgtttgcc
    43261 cgtgaacgta agatagacac gttggcctat atacctgatg aagatgaata taacctggtt
    43321 gtttctgcca ttaaaggaga tattcccact gcaaaatcat taatagataa aggcattaat
    43381 gtaaatactt ctattgacga aaacatgacc ccgctggtgt atgcggctat ggggggccag
    43441 gaaaagatga ttgtatatct cattggcaaa ggggcagaag tgaattaccg ggccactcag
    43501 gggcctacgc cccttgttgc tgcagtgagg tcaaaacaca ggtcgatagt tgatttgcta
    43561 attagtaagg gtgcagaaat agacatacct gatgccaatg gccgtacccc ccttatgtat
    43621 tctattcttt cggaagatac cttgttgttt gagaagttta ttaaggccgg agcagatatc
    43681 catcacaagg acaccactgg aattgatgta ttgatgtctg cggtaatcaa tgaccaaaaa
    43741 tattttgttc agaaactcat tgaacttggg gctactgccg atacttatga tgaaaacaat
    43801 gttactcctc ttatggtagc tgtaagccgt ggagatattg aaatggcaac tattcttctg
    43861 gacaacaaag cagatataaa ccataaaagt cataatcaac aaaccgcatt gacggtagct
    43921 attgagaata tggatgaaca aatggttgat tttcttataa aaagaggggc agatgttaac
    43981 cagaaattga cttttgctga aaaaccgagt actgtagcta attacttcaa tgtaaatgct
    44041 tttattaaag acgccttata tagtgcaggt gcaaaaaaaa cgtggttgcc tgattaccga
    44101 aagtttgttg ctggccctct aatattaggc aacatggatg aactgttcac tggtattgaa
    44161 gttggcctta aagattacag gtataatagt gatttgctcc tgggttttat cataaggcct
    44221 tttccaatca gggtacaagc cgattttgaa ggccgtacct accagttttg ggaagggcgc
    44281 tcggcatttt ttcttggcat agaaaaacga ttggccttga tacgaaaagc aaatgaccag
    44341 caactgggat ttttgtttgg ggcaaagggt gcatacacct ttggcaatta cagagggaca
    44401 acattagatt ttgaaaattc aattgttcct atgtttaatg ccggtgttta tgtctccaac
    44461 aggtatttgc agatagggct tagctatgtt tataccaatt ttaaagccaa aacggtttcc
    44521 aacaacagga tggaactttc ggtcaggcta tatttaggga aagcttttaa tttttcaaaa
    44581 aatgattatt cgccatggta aagttgttat atagaatcac gtttttattt atcatagtaa
    44641 atatgctcat tatggtaagc tgttctaatg aaacagtagg agaagactat tattgcgttg
    44701 attgtatttc ggaagaaccc gatacggcac agttaatagt taaagtttcg tatcaacaag
    44761 acaaagacag tataaaacta ttggtatata acaacaagtt ttcattagac acaaagcttg
    44821 acaccatctt gaatgatact attgctaaag atgtgctgaa gttttatgtg aaagtaaatg
    44881 aatactattc tgccgaagct atttattatg ttgggggaaa agaaatacat gccatagatg
    44941 gcggatggtt cgaaactaag aaaatagaag gttgccagaa tacctgctgg aaattgatag
    45001 gtggagatta cgatttacgg ttaaaataaa aaatagatgt atttgcatct caaaaaacgc
    45061 aatacttttt tcattatttg cctgttttta tgcatgatga cagcaggctt tttttcttgt
    45121 aagtttgatg agaaccctga tgaatatttg tttacaacaa aggaagtaac taaagatatt
    45181 gattctttag atgtttggac taagtctaat atttatatca tccgtaaaaa ggattttacg
    45241 gtaaaaggaa ggcttataat ccaggctggt actattatta aatttgaccc tgctgttaaa
    45301 ccctcaataa ttattaccga ttctggttca ttgtcatgca tgggcaccaa tgcaagtcca
    45361 attatgttta cctcgcttga tgatgatgaa aaaggggggg attcaaataa cgacaagcag
    45421 cccaggaagc ctgccgctgg tgattggggc gaaataatta ttagaagcaa agaattagct
    45481 gtaattaata attgttactt tctgtatggc ggtggcagca ctgataaaag tacaatagtt
    45541 gcagatagcg ctgctgtaga tatcagcaat tgtgtatttg gatacaacaa cggcggtaat
    45601 ttgtacgaag ggaatggggt gctgaatttg cttaaagcta aaaccggcac cagggttagg
    45661 aattgttatt ttcattcggg gaatttaccc atggtcatag cttcaaactt ttctattgat
    45721 aatactaacc agtttcacaa tcctaaagac tctacccaaa aaaataatta taatgccatc
    45781 aaaatagccg gaaatcaagc tgttcagggg caggttattt ggaacgaaga aaatgtgcct
    45841 ttttatgtaa gtactgatat tcttgaaatt tcaggtaata acaaccttac attgggaaac
    45901 aatactgtgt tgaagtttaa agctggtgct aaactcctgc tggacaatgg ttacagctcg
    45961 ttgttaaatg cagatgggcc gggagtattc cttacatcgg tttatgacga ttaccgaaaa
    46021 ggcgatacca atggggataa aaaccaaaca ttacctgcga atggggattg gttgataaaa
    46081 atatactccg attcggcagc attcaaccgt tctcatcttt attattttgt tgaaaactga
    46141 actatctatg tttgaaaggt tcgaaaaata cctgaagtct ttctgcaaat gtcttccgac
    46201 agacaaaata atagttgccg tgagcggtgg ggctgactca atgagtttgc tgcacatgct
    46261 ctataaagca aagtatgata ttatcattgc ccattgcaat tttcagttac gtggcgaaga
    46321 gtcagacaac gaggagcaat tggtaaggca gaccgctgct aagcttgaca tcccgattta
    46381 cgtcaaacgc tttgaaacaa ctgagtatgc cagcctgaaa aacatcagca tccaaatggc
    46441 agcaagggaa ctacggtata gttggtttga aaacctcagg cgtaatctgt catatcataa
    46501 aattgccgtt gctcacaatg ctgatgacaa tattgagact tttttcatca atctaatcag
    46561 ggggacaggt atagctggtc ttactggtat gaaggctgtt tcgggaaata ttgtacggcc
    46621 gcttttattt gctacccgcg aagagattga agcttattgc caaactgaga atattgtttt
    46681 tgctactgat tcatctaatc tgtcggacaa ataccttaga aataatatcc gtcataacat
    46741 agttccgttg ttaagacaga tgaacggggt gtttgatcag accatgttgg ataacttaaa
    46801 gaagttgcaa ggggcttacg ctatttatag tgatgcaata aagaatatta ttaatgaaat
    46861 tgtatcagca tcatctgaaa gcatgatatt aattgatatc agagcattgt taaatacagt
    46921 agaaccatca acagtcttgt ttgaaatatt gaaggagtat aatttcagtc cggtggttgt
    46981 ttccgagatt tataacagcc tcgatgccga cagcggaaag cagtttttct ctgaaagcca
    47041 ttggctggta aaagaccggg ataagctgat tgttagcagg ctaagtgaag ataaggctaa
    47101 caacgaagac gtattttata tctttgaaga tacggaacat ataaacatac caattagcat
    47161 gtctttcatc aaatcaagtg ctgaaggata tgaaattaaa aagaacccca atattgcggc
    47221 tttagattat gataagcttg tttttccatt gatattgagg cattggcgac atggagatta
    47281 ttttatccca atggggatga agggaataaa aaaggtaagc gacttttttg ttgataaaaa
    47341 gctatctttg cctgagaaag aaagtgtatg gcttttgttc agtgccggag agcttgtatg
    47401 ggttacaggt atgcgtattg acgagcggta taagattacc aatggtacgc agcaggtgta
    47461 tgaggtggaa gtaggggagt aggggagtag aagagtagaa gagtagaaga gtagaagagt
    47521 agaagagtag aagagtagaa gagtaagaat atacttaaac aactatcaac taaatatgag
    47581 cgacaataat caatacgaaa aaataatagc ctcaattgct acacaaaagg aagcttggat
    47641 actgcagaca gacgagggac ttattgcaat gtttgaagac aacgacatga atagctacat
    47701 ccctgtatgg acaagcaagg aacaagccca gaaaagtgca gacgaagact gggaaggcta
    47761 tacggctggc agaatgggta taggcgagtt tgttgaatgg atgcaggaac ttaaggatga
    47821 cgaaatcctt gtaggcctat attctgatgc aaaccaggca ttttctgtag atcctatgga
    47881 attgaaaaaa cagttgttgg caaaaaaata aaagagagta taaaatattt tgtgtaaaca
    47941 caataatttg atttttaatt tatttt
//
LOCUS       DAOSPF010000024        46005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_850_length_46005_cov_6.962307, whole genome shotgun sequence.
ACCESSION   DAOSPF010000024 DAOSPF010000000
VERSION     DAOSPF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 46005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_850_length_46005_cov_6.962307"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..413
                     /locus_tag="PK252_06570"
     CDS             51..413
                     /locus_tag="PK252_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14408.1"
                     /translation="MTCHKYFSFKNKNIPFIDFHHLLGLREEQEETASNVLVLDLNIN
                     NRNKLLGISVDEILEISELDDFLSYPYMPLINNRPFDFREAIIIHNNQPVIILNTHKV
                     WAKQIKKEMIFHTGNYPN"
     gene            420..800
                     /locus_tag="PK252_06575"
     CDS             420..800
                     /locus_tag="PK252_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14409.1"
                     /translation="MVQKKKIMLIDDSSTNNILYESILSEEGFEVTVVSNPLLAIQNI
                     KSEKPDLIILDLRMPVLDGFQIMEKIRGDKEISSIPIIVLSAENSREAEKKSYDLGAA
                     LYLSKPIGIDDIIQIVSKHMSATA"
     gene            complement(804..1385)
                     /gene="nadD"
                     /locus_tag="PK252_06580"
     CDS             complement(804..1385)
                     /gene="nadD"
                     /locus_tag="PK252_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOT14410.1"
                     /translation="MMIKTGLFFGSFNPVHIGHLAIANYMVEFTDIDQVWFVVSGQNP
                     LKKRKSLLADYHRLALVKEAIDDDHRFKASDVELKLPKPSYTIDTITYLKEKYPEREF
                     VIIMGSDGLTTFDKWKNYKLLEKSVCRYVYPRPGVDLQSFDNYPNCIFTNAPMIDISS
                     SFIRESIKNKKDVRFFLHEKVYKYISEMHFFEK"
     gene            1518..3668
                     /locus_tag="PK252_06585"
     CDS             1518..3668
                     /locus_tag="PK252_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14411.1"
                     /translation="MAYNSKTRVEKANLKGYVLVISMLFVFFNLFAMKGEQIQKNIVF
                     NVDTNSYNSVSNSFFFKGERYLWFEFNKTDEDIEIRVLSDKLNAKNIILMPSAEFSQI
                     DSPAFLNNEYYSFHIRFRNIARSNFLSLRFKVYEDSVNYQIEEVKLMPVNKTYVNLYT
                     NDYDLFIGEEKIFELQCNMPENLRLSEEWVITPEMDYKIYKKNNQLLLSIVPKSLGKK
                     NFSATIPTFKPIDLGIKSLNYECVIKPITFTVKSGRLAFLNLDQKEITFNDLSRKEGI
                     EVQIDKNRDMQIGKTYRIEEQETPGGALIAEIYTRQLLNNDKILCRLRVYNLHKPSDG
                     YLYIKDGDIAKFITNFIISPQTSIQKISVLKNGVDWNESNIVFPGEDITIRLEGIGFH
                     KAKFLFEEIKDLRQDTVVKTDNAVEYRLKIPADISKKKIFIYNDNENTGRFLQVKEYQ
                     EPRRMDFILVDYGNGPKAVEQYAGPELYEKTIKNIIISFDPDKIDSINKFYGKQYVDI
                     SVKITGSKNELLELTTLPQMVICPGPKSARNIYYDKRDATNDVYSLNTLLGQKTYDLD
                     EWAKIKITFRHSKEKYNSPGLERNVEIILQRKIKFDIDVSFPSLLTKVVKDNNWQNGL
                     GVSMAMIAQFSFYQKDKIARLMPYKVGVGFLALNAFNFSENITRDLGIVILGSVYPTR
                     RDTKFSFPLYAGGGYLLNRKSWFWLFGPGIRVSF"
     gene            3879..4964
                     /gene="prfA"
                     /locus_tag="PK252_06590"
     CDS             3879..4964
                     /gene="prfA"
                     /locus_tag="PK252_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT14412.1"
                     /translation="MSENVILSKLEGVRLKFEEVSQQITDPAVIADMKRFVKLNKEYR
                     ELEPIVAACNDYKNKLSNIETNKAILAEEKDEELREMAKMELEELEKELPELEEKIKL
                     LLLPADPEDSKNAILEIRAGTGGDEASIFAGDLYRMYTRYCESKGWKISVTSISEGTV
                     GGFKEIVMEVTGEGVYGTLKYESGVHRVQRIPKTETQGRVHTSAATVAVLPEADEFDV
                     ELNMNDIRKDTFCSSGPGGQSVNTTYSAIRLTHIPSGIVVQCQDEKSQIKNFEKALVE
                     LRTRLYNLEYQKFIDEIAKKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINYTVYNLPS
                     IMDGNIQEFIDQLQMAENAERLKESEI"
     gene            5072..5899
                     /gene="pyrF"
                     /locus_tag="PK252_06595"
     CDS             5072..5899
                     /gene="pyrF"
                     /locus_tag="PK252_06595"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT14413.1"
                     /translation="MNYLQLFENIKKKKSFLCVGLDTDINKIPAFLKSNEDAVFEFNK
                     QIIDATAEYTVAYKPNLAFYETTGVAGWVSLEKTVKYIKQNYPNIFLIADAKRGDIGN
                     TSKMYAHAFLQGLDFDSITVAPYMGKDSVTPFLEYKDKWVILLALTSNEGSFDFQLIK
                     NAETGDLLYQEVLKKSQQWGNKENMMYVVGATRAEMLADIRKIVPDHFLLVPGVGAQG
                     GSLAEVAKHGMNNHCGLLVNNSRGIIYADTSENFAKVAGAKAKEMQLEMETLLAKYL"
     gene            5927..7120
                     /locus_tag="PK252_06600"
     CDS             5927..7120
                     /locus_tag="PK252_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT14414.1"
                     /translation="MDNKTPLSELNFRMERFKQNLTAASPNWQYAAIFSKINILYFTG
                     TMQEGMLFIPRNEDATYWVRRSYDRAIDESLFPSIKPMDSFKDAGSAYQLTDKTVYVE
                     TEFLPIAFVQRFKKYFPFENFLSLDFQVAMTRAVKSQYELDFMIESGKRHKVILEDRV
                     PGILREGMNEVELTGELYKMMLEEGHHGVARFSMFDTEAGIGHIAFGESSIYPTSFNG
                     PGGNYGMSAAVPHVGSYTNRLKKGQLAFIDIGCGVNGYHTDKTMVYAYGQDVSPEVRA
                     IHNQCVDIQNRIAERLKPGEIPSKIFEDIMGSLSPEFMKNFMGFGSRQVKFLGHGIGL
                     VIDEFPVIAKGFDMPLTEGMVFAVEPKKGIAGIGMVGIENTFMVGKEGGICITGTHPG
                     LMVVK"
     gene            complement(7122..7559)
                     /locus_tag="PK252_06605"
     CDS             complement(7122..7559)
                     /locus_tag="PK252_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HOT14415.1"
                     /translation="MYSKGEISVLFEEPFWIVLFEKEDEYGYAVAREIIGASEPTGAE
                     LAAFLENINIDNLRFTLPLITQQKHGNFKRANYKLQMRKAKHIQETSKYKYTFTKAQT
                     MLKEHQSIQKEERKKFSRQEKEKDLERKFELKQIKRKEKHRGH"
     gene            complement(7559..7942)
                     /locus_tag="PK252_06610"
     CDS             complement(7559..7942)
                     /locus_tag="PK252_06610"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT14416.1"
                     /translation="METIKNIENGRLYTINTTERNDNKQWNAHPAFKGVYLKHLITGA
                     ETQGQFSAHIVKVEPGCVLDTHIHAGKTEIHEVIVGTADCFIDTKRVVYKPGDIAIIP
                     NDTKHKVIAGDEGLYLMAKFYPALL"
     gene            complement(8012..8803)
                     /locus_tag="PK252_06615"
     CDS             complement(8012..8803)
                     /locus_tag="PK252_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT14417.1"
                     /translation="MSKIKYYKSIDKAISIVCGHGVSKKFPVHRHLSLSVGMVVSGSR
                     ILTINQSKYVVAEGDIFIINSEEPHSISETNDAGHDYIVLSISLEYLKKFYTQDRLYF
                     FNIVENAVIAENLSLLFNSLINDKEIENVNFYIEEIIRSLQFCIDARNVINHEDDRLK
                     RIRYILDNQIDVPISLEELADKAFLSMFHFSRLFKKLTGLAPHQYLLDNRLRAARNLL
                     ERKMSIADIAIMTGFYDSSHFVRHFTKYYGVSPLDFQKGTNILHL"
     gene            complement(8864..9622)
                     /locus_tag="PK252_06620"
     CDS             complement(8864..9622)
                     /locus_tag="PK252_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT14418.1"
                     /translation="MKKLYADTSYTNLKKKVVKEFKHAKPGHWGEFVKGVDEKISTKQ
                     KLIAFTFDACGKEKGKGYDYELIQLLRKENVPATLFVTGRWIDKHFKALVALSQDSLF
                     EIENHGLNHKPCSACGESEYGIKGTANPSEAFDEIEANARKIEAIIGKKPRFYRSATA
                     FIDESCARMATKLGITVVSYEVLSGDAVANTPDTIIAQNVIKHAKPGAIVIMHFNHPK
                     WNTCEALRIIIPALREQGYKFIRLKDFPLKSMKD"
     gene            complement(9780..10580)
                     /gene="lpxA"
                     /locus_tag="PK252_06625"
     CDS             complement(9780..10580)
                     /gene="lpxA"
                     /locus_tag="PK252_06625"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOT14419.1"
                     /translation="MSFHPLTNVHPEAQIGQNVIIEPFATIEKDVIIGDGTWIGAGAV
                     IRNGARIGKNCKIDSCASIAGLPQDLKFRGEYTTVEIGDNTTIREYVTVNRGTAAKNK
                     TVIGNNCLVMSYVHVAHDCLIGNNVILAGYTGLAGEVEIGDWVITGGSTVVHQFVKIG
                     AHAFISGGSLVRKDVPPYVKAAREPLAYVGLNSLGLRRRQFSNETINIIQEVYRCIYL
                     RNMNHSQALEYIETNLPAIPERDEIVMFIRNSKRGIMKGYETDSGDND"
     gene            complement(10610..12004)
                     /locus_tag="PK252_06630"
     CDS             complement(10610..12004)
                     /locus_tag="PK252_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOT14420.1"
                     /translation="MAEKQKTLAAEVHFSGKGLHTGLMVNMYIKPAAANHGIRFMRID
                     LDENPIVHAIVDNVIDTSRGTTLDENGVRISTLEHLLAALTGLGVDNALIEIDAPEVP
                     ILDGSAKPYVEGIEKVGIVELEAEKKFFELKERIEFHDVEKGIEIVAFPDDHFSITVQ
                     IDYNSKVLGNQYASMNSLKEFAKEIAPCRTFVFLHELELLAKHNLIKGGDIDNAIVIV
                     DRKVEQEELDHLSALLNKPKFSVQPEGVLNNIELRFRNEPARHKLLDMVGDLTLVGMP
                     IKARIIASRPGHHANTEFAKLLRKIIKKESLKPAVPKYNPAENPLLDINQIMKILPHR
                     PPFLLVDKIIERTDQMIVGIKNVTMNEPFFVGHFPGEPVMPGVLIIEALAQCGGLLAL
                     NTVEDPETYSTYFLKIDKVKFKKKVVPGDTLIFKLELIEPIRRGIVTMWAQAFVGNAV
                     VTEAELCAQIVKTK"
     gene            complement(12031..13074)
                     /gene="lpxD"
                     /locus_tag="PK252_06635"
     CDS             complement(12031..13074)
                     /gene="lpxD"
                     /locus_tag="PK252_06635"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOT14421.1"
                     /translation="MKFTAQAIASFLKGEVVGNPDVEVSDIAKIEEGRPGTMAFLANP
                     KYNKYLYETKASIVLVNKTLQIEGPVSATLIKVDDAYQCFAALLEMYQQAKPQKSGIE
                     SNCYISESATLGTDLYIGAFAYIDNNVKIGNNAKIYPQAFIGENVTIGENTVIYPGVK
                     IYADCKIGANCVIHAGAVIGADGFGFAPNSENNYKKVPQIGNVVIEDYVEIGANTTID
                     RATMGSTIIRKGVKLDNLIQIAHNVEIGENTVFAAQTGVAGSTKVGKNCMFGGQVGLA
                     GHITVADGVKLGAQSGVNASITKEDATMIGAPAFDFKEYMKCYVLFRNLPKLNAQLNQ
                     VERDIKEIKNKNQ"
     gene            complement(13171..14427)
                     /locus_tag="PK252_06640"
     CDS             complement(13171..14427)
                     /locus_tag="PK252_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT14422.1"
                     /translation="MNTNKRKIVNDPVYGFISINNDLIFDLIEHPWVQRLRRIRQLGL
                     TNLVYPGAMHTRFQHVLGAYFLMNQAIEVIRSKGHEITDEEAEAVNIAILLHDIGHGP
                     FSHALEFGIIEGFTHESISELLMAGLNAQFNNRLTLAIAIFQDKYHKRFLHQLVSGQL
                     DMDRLDYLRRDSFFTGVSEGVVGSDRIIKMLNVVDDKLVVEAKGIYSIEKFLIARRLM
                     YWQVYLHKTVLSAENLLINILKRAKELSLQGDKLFAPPALEYFLFKKFDRSFINAADE
                     KDLNTMLQHFVRLDDTDIISATKVWMEHDDKILSTLCQKMISRQLYKIELQNQPFSAE
                     RIAEMIEKTEKHFSLSSKEASFMVFADSITSNIYETGEHNIGILYNNGKVVDIAEASD
                     MLNMQVLTKVVTKHFLCYPKEIISKI"
     gene            complement(14847..16478)
                     /locus_tag="PK252_06645"
     CDS             complement(14847..16478)
                     /locus_tag="PK252_06645"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT14423.1"
                     /translation="MPTPPFYYQELFPLEKDNTEYYLLSKEHVSVKQFDGKEVLCVNP
                     EALTMVTNAALRDCSFLLRTEHQQQVAAILDDPQASENDKYVALTMLRNAEVSAKGQL
                     PFCQDTGTATVVAKKGHLVFTDGNDAEAISLGVYKTYTEENLRYSQTVALDMYNEKNT
                     GTNLPAQIDIYCEKGDEYKFLFVTKGGGSANKTYLYQETKALLTPGKLENFLIEKMKT
                     LGTAACPPYHLVFVIGGTSAEACLKTIKLASTKYLDTLPTTGNEHGRAFRDVELEQKI
                     LKAAQQFGMGAQFGGKYFAHDVRIIRLPRHGASCPVGMGVSCSADRNIKAKINKDGIW
                     VEKLEDNPGRFIPERLRNVGEGEAVKINLNRPMKEVLAELTKYPVATRLSLNGTIIVG
                     RDIAHAKLNERLEKGEGLPQYLKDHPIYYAGPAKTPAGMASGSFGPTTAGRMDSYVAK
                     FQENGGSMIMIAKGNRSPEVTEACKKFGGFYLGSIGGPAAILAQQNITKVELLEYPEL
                     GMEAIWKIEVVDFPAFILVDDKGNDFFKQLKPWNC"
     gene            complement(16532..16882)
                     /locus_tag="PK252_06650"
     CDS             complement(16532..16882)
                     /locus_tag="PK252_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOT14424.1"
                     /translation="MEKLKIYHNPRCKKSCEGLNYLKEKGVDFEVVKYLEKGISSDEL
                     KEIFLKSGKKPVELVRTQEPEYKIELKGRNFSDDEWVQIIIDNPKLLQRPIIVGKYKA
                     VLAQPPSNADILIK"
     gene            complement(16893..17982)
                     /gene="prfB"
                     /locus_tag="PK252_06655"
     CDS             complement(join(16893..17927,17929..17982))
                     /gene="prfB"
                     /locus_tag="PK252_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113942210.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT14425.1"
                     /translation="MITTDQIKDLKEREDALRGIFDIERRLIELEEEEQKTHAPNFWD
                     DPKEAEAQLKKVSSIKQWTDHYAKVKSAVEDLEVMFDFAKDGQAEESDVDAQYAQALK
                     IVEDLETRNMLRNEEDKFGAILKINAGAGGTESQDWADMLLRMYLRYGERHGYKMKEV
                     DYLKGDEAGIKSATIEFDGEFAYGFLKGESGVHRLVRISPFDANARRHTSFASVFVSP
                     LVDDTIEIVINPADITWETYRSSGAGGQNVNKVETAVRLRHAPTGIVIENQEDRSQLK
                     NKENAMRILRSMLYELELQKRREKQAEVEGKKMKIEWGSQIRNYVLHPYKLVKDLRTD
                     YETSNAQDVLDGGLDGFIKAFLMEYGGQ"
     gene            complement(18040..22260)
                     /locus_tag="PK252_06660"
     CDS             complement(18040..22260)
                     /locus_tag="PK252_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT14426.1"
                     /translation="MKKKKEMDVMRKISIITVFLFLSFIVNAADNDNFDNFRFEHLTI
                     SDGLSQSAVNEITQDCFGFVWIGTQDGLNRFDGYEVKVYKNDPQDKNSLTNNQILAIT
                     SDTSGNLWIGTFGGGLNIYNPHCDSFLNIINRPEFFKNISGNEIMRVFRDSKGNMWVG
                     TEIGLYRARAAANSKSVTEIRFERVQLTINKQLANYQVRSICESTDGKILIGTLGGLY
                     YVDMTGNTLSTLYPFSELAFPTVIALHQQKNGILWIGTLENGLYKLQNIQGKVQVTHQ
                     NNISFIRVTAITEDKKGNIWVASRQGLAKIAKDGAITYYKADNTSENAISDERLNCLF
                     IGNTNILWIGSEYKGLNWLNLSRKKFHTIKNIGSTQNALSTSFVTAIHSSQPNSVFVA
                     TDGGGIDKLSFDPLSGMFLNIEHWNRNLSGKDKQVISLYQDKNGSVWFGSAQNSINII
                     NSITQQLKTLSIFGYVFTIVEDSYGYIWLGTWGQGLYRYNKAKATLEHYKLNLSDEFS
                     TEGDIIFNLFEDSEKKLWIGTKGNGLHRLLNPSAAPDKARYKHYYHDKNKKKSLAHND
                     VYVVTEDKEKNIWIGTVAGLNKLSLKALNDNVEPEFTIWNEKNGLPNSVVYGILVDND
                     NNIWLSTNKGISKYLVKKHYLVNFDVNDGLQSNEFRQNAYFKDNKGRMYFGGIAGLTY
                     FNPSEIDLDTSKTNVVFTSLKINGVDVPINQSFNSRVLLNSSILATKTIELKASEREF
                     SIGFTAVNFTIPEKIQFQYRLLGFNEQWQPISNRIRNVTYTNLSPGDYELQVKATNSD
                     GVWSEKYTSMKIVVLPPLWRTWPAFLLYFMVLVVLYILFKKYSLIAVKKKNQLLIDSL
                     EKDKIEELTKLKLQFYTNISHELRTPLTLISSPIEDLIHDESVDNKVKAQLSVVYKNV
                     QRLLHMVNQLLDFRKIDAGQVKLTLSYENLVLLLNDVYTVFDQYALSRNINLQYIHKP
                     SEVMAWIDREKILTVMFNLLSNAFKYSPDNSLITLELCYEQPTENEQEDGGQTLPNIL
                     IKVIDNGCGISSEHLDKIFDRFYRVHNEKGQFIPGSGVGLSIAREFMLMHGGNIQVES
                     EPGKGSCFTITIKQGYRHLLHIESPEYFEDKSGAQKEPANDSSQQYLERMANSLNEKV
                     QASLQVENVLLLAEDNLELLDYISSKLSQKFKIVTAPNGEDAFKTALSINPDLIITDL
                     MMPVMDGLTLCKKLKSDIHTSHIPVIVITARSASDANIEALESGAEVFIQKPFSVDVL
                     KAQINSILDSRKRIQNSINKQLILQPKDIAVTSLDERFLKKLMEVVEQNLANPDYGIK
                     ELTHDMAMSHSVLFRKIKSLTGMNIVEFIRSVRLKKAALILEKNKIPITELCSMIGFN
                     DPKYFSKCFIAQFGLTPTEYAAAKGEGK"
     gene            complement(22519..23721)
                     /locus_tag="PK252_06665"
     CDS             complement(22519..23721)
                     /locus_tag="PK252_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT14427.1"
                     /translation="MPKISQRGIDMPQSPIRKLMPYAEEAKRKGIKVYHLNIGQPDIP
                     TAPLMLEAVKSYVGRVVEYSHSAGNESYRKKLAQYYCKQGINVDYNDVLITTGGSEAI
                     NFAFLSTMDPGDEIIIPEPFYANYNSFASAVGVHVVPVTSSIQNGFSLPPVEEFEKVI
                     TARTRGIMLSNPNNPTGCLYSRDDLEELRRIVKKYDLYLYADEVYREFCYTGEKFFSV
                     MELEGMEQNTILIDSVSKRYSACGVRIGAMVTRNKEVVNIAMRYAMARLSPPSFGQMA
                     GEAAIDTPAEYFEVAYNEYLARRNFLVERLNKIKGVFCPLPKGAFYTIAHLPVDDAEK
                     FATWLLKDFEYNNQTVMLAPAAGFYATPGLGKSEVRIAYVLNINDLTNAVECLKKALQ
                     EYPGTKVK"
     gene            complement(23745..24158)
                     /locus_tag="PK252_06670"
     CDS             complement(23745..24158)
                     /locus_tag="PK252_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOT14428.1"
                     /translation="MKRFFLVTVTIAVATFLSCTHASNEGNKKAAIIAFADTEHDFGT
                     IEQNSVAEYAFVFRNTGKEPLVIDNVHTSCGCTVPEWTRDPVKKNRSGVIKIHYNTHV
                     VTSFNKTITVYSNAANSPVTLTIKGTVVPKTEGKK"
     gene            complement(24382..25263)
                     /gene="folD"
                     /locus_tag="PK252_06675"
     CDS             complement(24382..25263)
                     /gene="folD"
                     /locus_tag="PK252_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOT14429.1"
                     /translation="MQIIDGKKVAEQIKLEIAEEVKQMKEQGLKIPHLAAILVGHDGG
                     SETYVAHKVKDCAEVGFKSTLVRFEDDVTETELLAAVQKLNNDSDVDGIIVQLPVPKH
                     ISEQKIIEAIDYRKDVDGFHPINVGRTVIGLPSYVSATPFGIVELLRRYKVETKGKNC
                     VVIGRSNIVGRPMSILLSQKAQPGDCTVTVCHSATPDIASYTSKADIIIAALGKPGFL
                     KASMVKEGAVIIDVGTTRVPAPETKSGFRLKGDVDYDEVAPKCSFITPVPGGVGPMTR
                     VSLLQNTLLAAKKSFFK"
     gene            complement(25362..26492)
                     /gene="tgt"
                     /locus_tag="PK252_06680"
     CDS             complement(25362..26492)
                     /gene="tgt"
                     /locus_tag="PK252_06680"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT14430.1"
                     /translation="MKFILHNTDKQSAARAGTIETDHGIIETPIFMPVGTAGSVKGVH
                     QRELVNDIGAQIILGNTYHLYLRPGMQIMKDAGGLHRFIGWDKPILTDSGGYQVFSLS
                     ENRKLKEDGAHFASHIDGSKHLFTPENVVDIQRIIGADIIMAFDECTPYPCEHRYAEK
                     SLKLTHQWLDRGVARFKETEPLYGYGQTFFPIVQGSVYKDLRKISAEKIASVGMEGNA
                     IGGLSVGEPAEMMYEMIEVVNDILPKDKPRYLMGVGTPANILEAISLGIDMFDCVMPT
                     RNGRNGMIFTRNGIMNMRNEKWKDDYSPIDAESDCYVDQVYSKAYLRHLVVSKEMLGA
                     QIATIHNLHFYLWLVKEARKQILANTFASWKVETLRKISARL"
     gene            26805..27923
                     /locus_tag="PK252_06685"
     CDS             26805..27923
                     /locus_tag="PK252_06685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT14431.1"
                     /translation="MTTLEITGLATLSVLILCSAIQYIFYWFIFTKPFSIKNKTSEAS
                     HPVSIIVCARNEEANLRENLPLLLEQEYANYEVVVVNDCSSDDSDLVLKALAAQYPHL
                     RYTTIKENEKFQHGKKLALTVGIKAAKNEWMLMTDADCKPSGPHWLSAMASQMTEDKA
                     IVLGYGGYLSQPGLLNKLIRFETAWIALQYFSLAIIGNAYMGVGRNLSYRKSLFFANK
                     GFASHARLSSGDDDLFINETATATNVSVQFLPEAHTYSKPKKSLSEWIEQKSRHFQTY
                     PQYKFKHKFLLTAETFSRVTFYFSLAALFILQQFIWVALALLFVRLVSQVIVLNVWFK
                     RLNEKKLFLLSLIFDLVMPFLYFIIYLTKKIKPARQWR"
     gene            27914..28519
                     /locus_tag="PK252_06690"
     CDS             27914..28519
                     /locus_tag="PK252_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT14432.1"
                     /translation="MEVNTNLSDKAQADMALVQRATAGDQRAFAELLSRYRDSIYFML
                     LKMVNNQDDAEDLTIEAFGKAFKNIHQYTPNYAFSTWLFKIATNNCIDFIRKRRGGIT
                     ISIDQGFANYEDDLPIHIHDIEPDPEANMINEQKVKVMREVVQKLKPRYRKLVEMRYF
                     DELSYEEIAEQLDLPLGTVKAQLFRARELLFNILKTSEHKI"
     gene            28519..29157
                     /gene="rsmG"
                     /locus_tag="PK252_06695"
     CDS             28519..29157
                     /gene="rsmG"
                     /locus_tag="PK252_06695"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT14433.1"
                     /translation="MNIVLKYFPELSDNQKRQFAALYDLYFDWNAKINVISRKDFEHI
                     YERHILHSLAIAKFYKFPAGTKIMDVGTGGGFPGIPLAIMFPECNFTLIDSIAKKIKV
                     VDAVANAIELSNVRPIAMRAETVPEKFHYVVSRAVTTIPEMISFIKKSFADENINPYI
                     KNGLIYLKGGDIDAEIKDYKNRTRVHEISEVFTEAFFETKKVIYIPAAAFAK"
     gene            29232..29531
                     /locus_tag="PK252_06700"
     CDS             29232..29531
                     /locus_tag="PK252_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT14434.1"
                     /translation="MYLDNYMESMKNLCQQNKVKALYVFGSVLTDRFNDKSDIDLIVD
                     IDSNDPLEYADNYFNLKFALQDLFKRPVDLLENKAIKNPYIRQNIDNSKHLIYAK"
     gene            29521..29862
                     /locus_tag="PK252_06705"
     CDS             29521..29862
                     /locus_tag="PK252_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT14435.1"
                     /translation="MQNDKKYDIRACLKDIELSIAEIYQFLPAKRNFQEFQKDLKTRK
                     AVERNIEIIGEAMGRILKADPTFPITDSRKIVDTRNRIIHGYDSVSEDIIWLIICKYL
                     PILEKEIKKFL"
     gene            29950..30648
                     /locus_tag="PK252_06710"
     CDS             29950..30648
                     /locus_tag="PK252_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT14436.1"
                     /translation="MKKLIFLLLLAPIALTAQNTDILPYIEVTGNAEDDVEPNKVVLS
                     LSVSENENIKKESNTVAMENKISAFLKSIGTDPANFVVDKYNTNERYTIISGKNVTIK
                     KYYKLTILNLSLLDTIITKCSEVGMDKISIFKIENTSIDSINNILLASAAKNARQKAD
                     AIASALGVSISNIQIVNANHFVEPNIVNANNFKMEKMLFSSEDAATGRTSSSLGLNKI
                     HLNASVFIRFGIKQ"
     gene            complement(30759..32465)
                     /locus_tag="PK252_06715"
     CDS             complement(30759..32465)
                     /locus_tag="PK252_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT14437.1"
                     /translation="MCLACGTCIKACSHGARYYVDDIERFLYDLKHGVKMVSVVAPAI
                     AANFPGQYLKINALLKKMGVEANFDVSFGAELTVKSYLEHLNNNNPRMIIAQPCPAIV
                     TFLEVYHPELLPYLAPADSPMMHTIKMIKEFYPQYKDHKVVVISPCVAKRREFDEVGM
                     GDYNVTIKYLKDFIDFNHINLEDFEDMDFDNPPAERAVLFSTPGGLLRTAEREVPTIG
                     KVSRKIEGHEVIYDYFESLIDEVQSGRAPVLVDCLNCHAGCNGGPGTLNQNEPLDKIE
                     YYVEQRNKEAQEKYKDAAELETNINSYWDEKIYKRTYVDRSANNKIRYPSESELQRIY
                     VDMKKLKETDFYNCAFCGYNSCENMAIAIYNHLNKKENCYHYKAAIISELANDVSKTV
                     NELDRQGGVVNAHVKNVQGITARLNAEFLSLLNEVDDNNSKLDEFSKVIETLNAIASK
                     TDLLSLNASIEAARAGEHGKGFSIVANEVKKLAENSQIEANKIKPYLTEIADLFIQIN
                     EKIKNTSKEFKSADKLNAEVRSSIESISEIISELNERTKAFVSHTQSILAEAEKQPVK
                     YN"
     gene            complement(32438..32596)
                     /locus_tag="PK252_06720"
     CDS             complement(32438..32596)
                     /locus_tag="PK252_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14438.1"
                     /translation="MINELSQVLLVDRKNVSTATSVYLFVLPSIVMMEVAILLLLTMI
                     CVWPVVLV"
     gene            33295..34146
                     /locus_tag="PK252_06725"
     CDS             33295..34146
                     /locus_tag="PK252_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14439.1"
                     /translation="MKTITKQITVLAVFGLISASAYSQANSYSVNSETLYRHGYNSGG
                     ANVQENTDSVTTGGTTRYWVYPDLSLNPGYDVNNPLTTPLTSAFNWTTSGATGTATGT
                     IAAVGAYGTYTNYKQVTWGGTGTINLNATETSGAGCVGGTAVTTPIAVIAAPDVTAIA
                     FSALPCPTGTIPYTQAGPTATLTITCPVNGNKEVSVNWSLSCSNAGWNGGVAMTGTAV
                     LGNGNTIDLSGVNLSHPGTYTLTIVNITDRIATKSGLTAIPDGTSNTFVLLPTPVTGP
                     MYHVPNQ"
     gene            34428..35258
                     /locus_tag="PK252_06730"
     CDS             34428..35258
                     /locus_tag="PK252_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14440.1"
                     /translation="MPYSVVPDSTIQTIVASGAPFQASSYNWRLGTTPLGSLRQADGS
                     TALANIATDPAPYNAGYYLDYDVAVIWGNTPGIDSIKVSEHSRNNGGLQTCDGADTTL
                     YVYVMPKPTAQFIEAGTLAGATNIVGTANDAIVGGCGIAGSTLHFNVTFTGTQDFYVY
                     YSSVYTPFGGLAAPAVTGSVTTGLGAANFYTATPTVPNTDAVTDAFTFAIPAATYGKY
                     VFTLTGVNDLVSRKSLTAADMGTLVNTTGSAPAGPTLTVYSLPTPTTGRVLHVTNQGW
                     "
     gene            35508..36611
                     /locus_tag="PK252_06735"
     CDS             35508..36611
                     /locus_tag="PK252_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT14441.1"
                     /translation="MLKTKHTILILLLLSVFAWGKAQVMDTVCTQSIVGFYTKPDALI
                     TDYHIMWHLDFDTSKIVDSKTTLNGDTLKIHFGNSKGWFRVGGQIISDIGPTGLNKCE
                     GDIVWDSVYVWGYGNIDLPDTLIRCENEPFVLAVDEGLRSYKWSSLNGNGNVYRGIAT
                     KSDTIWVSARDQHNCLLTDTTKLTVYPLPTFDILDYRSGKAIKDTMLCGTDQVTLDAN
                     SFATYLNWNTGEISQQIVVNPIELNTKDTVRYYKATAIMDYEYASCQYEDSVAVLACF
                     TGEIEGIPTVITPNGDGHNDDWNIPNLNRDPACTIDIYDRWGRLVFHSRGYAKNWDGK
                     GNDGKALPMDNYFYIIKLSGNTKTLIGNITIIR"
     gene            36732..37712
                     /locus_tag="PK252_06740"
     CDS             36732..37712
                     /locus_tag="PK252_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOT14442.1"
                     /translation="MRILTLFLLMLAIKTVSVYGQQEPSYTQYMYNMFLTNPAAAGSD
                     GSTSVSFTGREQWIGFPGAPKTHSLSAQTRVLKNSFIAKALNLRKKFSRRSSSGRIGA
                     GAHIFSDINGPVMRTGLHLTYAYHITIRQSQLSFGLTMMPYQFNTINSFFKNYDTNDP
                     LMINLKPRYVIDGNFGMLYTTPEYFGGLSVTNLFESNIKFGDNSTAGYQILRHYCIMG
                     GYKFPVNRYITIEPTSLIKFTEMGSFQMDITGRVFYRDDYWGGLGYRTGANAGAISLL
                     AGIRLKKYYFGYAFDLSLSPIQQYSYGSHEFIFMLKFGENARRYRWLNRY"
     gene            complement(37989..38720)
                     /locus_tag="PK252_06745"
     CDS             complement(37989..38720)
                     /locus_tag="PK252_06745"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOT14443.1"
                     /translation="MKREKSTVLVAIMLFFVGLISCSNVELIAGNGKIVKKEIDVKDF
                     KGVCVSAGLDVTLVQDSFERVVIETDENMLEYVKFENNDGVLNIYRSEGRSKPTKVKI
                     YVTAVNIEKLYASSGADIKGAGLIKTDYLEMDASSAADITVEVRGKYVVANSSSGADI
                     NILGEATVFNATASSGADIKAKSLIAGTTKANASSGADIAVCVKEEIIAEASSGGDVK
                     VIGSPIRRDVRLSSGGDVTFEKGGE"
     gene            complement(38896..40287)
                     /gene="lpdA"
                     /locus_tag="PK252_06750"
     CDS             complement(38896..40287)
                     /gene="lpdA"
                     /locus_tag="PK252_06750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT14444.1"
                     /translation="MDYDIIVIGSGPGGYVAAIRASQLGKKVAIVERSEIGGICLNWG
                     CIPTKALLKSAQVYSYAKHAAEYGIKLPGEAAVDFDAVVKRSRTVAEGMSKGVQFLMK
                     KNKIEVIAGFGKLKAVNTVEVEAADGSKKELTAQHIILATGARSKELPNLKQDGVKII
                     GYRQALTLPKLPKSMVVVGSGAIGSEFAAFYNTMGTKVTLVEFLPAIVPLEDEDVSKQ
                     LERSFKKDGMEVLTNSSVEAVQVKGDVCEVKIKTPNGEIVKEAEVVLSAVGITPNIEN
                     IGLENLGIKVEKGKVVVDEYYQTNVKGIYAIGDIVPGPALAHVASAEGIVCVEKIAGL
                     NPEPVDYSNIPACVYTTPEIASVGMTEKEAKEKGHEIKVGKFPYMASGKASAAGSKDG
                     FVKLIFDAKYGELLGAHLIGLNVTEMIAELVVARKLETTGHELIKAIHPHPTMSEAIM
                     EAAAQAYGEVIHL"
     gene            complement(40471..41271)
                     /locus_tag="PK252_06755"
     CDS             complement(40471..41271)
                     /locus_tag="PK252_06755"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOT14445.1"
                     /translation="MNYNCILGKLISEHTSPITYFLESGNEKININQWLGKLVRISYA
                     GEIYCIKCGKRTKTSFQQGYCYTCFTSAPETEECVFRPELCRAHEGIARDMEFAKAHC
                     LIEHIVYLASVDHIKVGITRHTQIPYRWIDQGAEKTRIIARTPNRYLAGCIEVALKQY
                     LPDKTNWRSMLTNKVEKEPSFDAIFDKVASVLPSDLLGYLSENEQVVDFQYPVNAYPQ
                     KVKSLDLEKQPDIEGKLSGIRGQYLIFEDGGVLNIRKFGGYKVSVNVE"
     gene            complement(41356..41799)
                     /locus_tag="PK252_06760"
     CDS             complement(41356..41799)
                     /locus_tag="PK252_06760"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOT14446.1"
                     /translation="MRIVVDTNIVFSALLSTNSYISQIILQPKTRLNFYSTERLIYEI
                     SSHRDKIISLTGYSNYDLDRMISLITSRIRFVNISLIPKEIYYDCELLTKDVDVDDCE
                     FVALTEHIRGKLWSGDKRLIRGLQKKKWIKFVNTDDLYRMIMKHP"
     gene            complement(41796..41999)
                     /locus_tag="PK252_06765"
     CDS             complement(41796..41999)
                     /locus_tag="PK252_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14447.1"
                     /translation="MIIERTNKEIIFRLPADTNVDELQDMADLLEYKEIAKKSKATQK
                     DVDELVKTIKKGRWAATKSTLNL"
     gene            complement(42062..42505)
                     /locus_tag="PK252_06770"
     CDS             complement(42062..42505)
                     /locus_tag="PK252_06770"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT14448.1"
                     /translation="MKKLLVPPVFVLLTLILTVAFYFCCPKFNVIDFPYNLAGLLSVV
                     LGFAIMSKTWDLFKKHNTTLEIKPSSSMITEGVFAKSRNPMYIGMFLLLLGFGICSTN
                     MLSILACFVFVLLVNGLVIPQEEKLMKEAFGQEYDAYKSKVRRWI"
     gene            complement(42551..43795)
                     /locus_tag="PK252_06775"
     CDS             complement(42551..43795)
                     /locus_tag="PK252_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT14449.1"
                     /translation="MGNKDLKGFHSRLLHAALSRPDAYGALQTPIYGCSAFEFESAES
                     IAGAFNGTMIAPAYSRTANPTVEHFELQVKSITEAIGVVAFSSGMAAVSAAIMNVCKA
                     GDNIVTTNKLFGNTVALYESTLNNFGISFKYADFTKPETIEPLIDGNTRAVLFEVLNN
                     PFVEIADVGSISAIAHKHNLVVLGDTTLTPPNMFNARKFGIDISLVSSTKYISSGAAV
                     IGGLVIDHGTYNWENNPKLKPFVPTSGQLTFLARLRKDILRNTGGCLSPFNAYLQSLG
                     IETMQLRFERMAANALAVATWLSNNAEVKTVNYPALKGSKYEAVCRKQFTALPGGLMS
                     FELESAEKCMKFINRLKLVKRATNMCDNKTLIIHPASTIYCDFTPEQQRAMGINEGLI
                     RLSVGIEETEDIIADFAQAFGQ"
     gene            complement(43850..44281)
                     /locus_tag="PK252_06780"
     CDS             complement(43850..44281)
                     /locus_tag="PK252_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT14450.1"
                     /translation="MKITNKSFYALKFMLNLATLPANGRLTVAGLAHKENISEKFLES
                     IVAMLKSNGIVKSKRGAGGGYLIGNSPSKITIKSIIVAVESDSKGFQPPYNEPALHTD
                     LVLRAMFDEMDESVGRFFENITLASLVEKMEMLGDNEMFYI"
     gene            complement(44308..44856)
                     /locus_tag="PK252_06785"
     CDS             complement(44308..44856)
                     /locus_tag="PK252_06785"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT14451.1"
                     /translation="MTNIFLVGFMGCGKSTIGKMLAQELGLQLVDMDAVIEEQQQMAI
                     SQIFATKGENAFREIEKDTLHILGKKEKLLIATGGGAPCFFDNMEWMNQYGITIYLKM
                     SAKALAHRLMSLSEKARLSRPLLAGKTESELAEYIEKTLKIREPFYGKSRIVILTDDI
                     NPQTTVSRIMTALNMMQAAKKV"
     gene            44949..>46005
                     /locus_tag="PK252_06790"
     CDS             44949..>46005
                     /locus_tag="PK252_06790"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT14452.1"
                     /translation="MENLDYIHSLINLCFAENKESFVHSYNELQQKSNWKNIAKVNFL
                     LPLVFYRAKKLGALSALQQKDSELADKQLKRASVKNLKLNHAFSKLYTIALNNNIEIT
                     AIKGVYLQHYLYPESYLRQMVDIDILVAASKVEELRKKLVIEGAIPITVHQSRFIESL
                     LHQISPIKLNDVSIEIHRNLFDSFDEFVCPEALIASNSRVETLSGHKVKVLNNEFLLV
                     YLCCHVFNTIRGGSLRLISLIDIFLLLNKSNINVEQLNSIAKEMNATLALQNILYLVS
                     KLFKVETPCCPSTPENIELFPISLDYAIKIFLSEQPPFDTSHYFTKFKKINGFKNKTK
                     YILHAVFPQQEYIKNRYL"
BASE COUNT        14326 a         9601 c         8041 g        14037 t
ORIGIN      
        1 agccaaattt gtttaatttt taataacgtc ccgattttaa tgggacacta gtgacctgcc
       61 ataaatattt ttcattcaaa aacaaaaaca ttccttttat tgattttcac cacttgttag
      121 gcctcagaga agaacaagaa gaaactgctt caaacgtgct ggttttagat ttaaatataa
      181 ataaccgaaa caaactactt gggatttccg tagatgagat actggaaatt tcagaattag
      241 atgatttttt aagttaccca tatatgccat tgatcaataa caggcctttt gatttccgtg
      301 aagcaattat tatacataat aatcagcctg taataatact aaatacgcat aaagtatggg
      361 caaaacagat aaaaaaagaa atgatttttc atacaggcaa ttacccaaac taaataaaaa
      421 tggtacagaa gaaaaaaata atgcttatcg atgattcgtc aacaaacaac attttatacg
      481 aaagcatttt gagcgaggaa ggatttgaag ttacagtagt tagtaatcca ttattggcca
      541 tacaaaacat taaatcagaa aaaccggatt tgattatttt agacctaagg atgcccgttt
      601 tagatggttt tcaaattatg gaaaaaatcc gtggcgataa agaaatttca tctatcccga
      661 ttattgtact aagcgccgaa aacagccgtg aagctgaaaa aaaatcatac gaccttggag
      721 cagctttata tttaagcaaa cccataggta ttgatgacat tatacagata gtatcaaagc
      781 atatgtctgc tactgcataa aagctatttt tcgaaaaaat gcatttcgga gatgtacttg
      841 taaacctttt catgaaggaa gaaacgtaca tcttttttgt ttttgatgct ttcgcgaata
      901 aaagacgaag aaatgtctat cattggagca ttggtaaata tacaattggg ataattgtca
      961 aaagattgca aatcaacgcc aggacgagga tagacataac ggcacactga tttttctaag
     1021 agtttgtaat ttttccactt atcaaacgta gttaacccgt cagaccccat aataatgaca
     1081 aattcccgct cagggtattt ttctttcagg tatgttatcg tatcaatggt gtaagacggc
     1141 ttgggcagtt tcaactcaac atctgaagcc ttgaagcgat ggtcatcgtc aattgcttct
     1201 tttaccaaag ccaatctgtg gtaatcagcc agcagcgatt ttcgtttttt aagaggattt
     1261 tggccactaa ccacaaacca cacctggtct atgtctgtaa actcgaccat atagttggct
     1321 attgccagat gcccaatatg aacagggttg aatgaaccaa aaaacaaacc agtttttatc
     1381 atcatttagt caatagttga acaaaagcaa agataaaaga acaaggctta gattttaact
     1441 tctaatgaaa aaagaaaatc aggcaaagaa attatttttg caacctaagt cttttgacca
     1501 aattaaataa taatactatg gcttataatt ctaaaacgcg tgtagaaaag gcaaatttga
     1561 agggatacgt attggtaatt agtatgctgt ttgtattttt caatttgttt gccatgaaag
     1621 gtgaacagat ccaaaaaaac attgtattta acgttgacac aaactcttac aacagcgttt
     1681 caaatagttt cttttttaaa ggcgagagat atctttggtt tgagtttaac aaaactgacg
     1741 aagatataga aattagggta ttatcagata agcttaatgc aaaaaacata attttaatgc
     1801 caagtgcaga gttttcacaa atagattcgc cggctttttt aaataacgaa tattattcat
     1861 ttcatatacg atttaggaat atagcaagaa gcaacttttt atcattacga tttaaggttt
     1921 acgaggactc tgttaattat caaattgaag aagtaaagct aatgccagta aacaaaactt
     1981 atgtaaacct ttacaccaac gattacgatc ttttcatagg agaagaaaaa atatttgagc
     2041 tacaatgtaa tatgccagaa aacctcaggt tgagtgagga atgggtgata acacctgaaa
     2101 tggattacaa gatatacaag aagaataacc aactgctatt gagcattgta cctaaatcat
     2161 tgggcaaaaa gaatttttct gctacaatac ccacctttaa gccaatagat ctgggcataa
     2221 aaagtttgaa ctacgaatgc gtaataaaac ctataacttt tacagtcaaa agcggccgat
     2281 tggcattttt aaatttagac caaaaagaaa ttaccttcaa tgatttgtca cgcaaagaag
     2341 gaatagaagt acagatagac aagaaccgcg atatgcaaat tggcaagact tacagaattg
     2401 aagaacaaga gacaccagga ggcgctttga ttgcagagat atataccaga caattactaa
     2461 ataacgataa aatactatgc cgattaagag tatataacct gcacaagcca agcgatggat
     2521 atttatatat aaaggatggc gacattgcta aatttattac aaatttcatt atcagcccac
     2581 aaacaagcat acaaaaaata agtgttttga aaaatggtgt tgactggaac gagagtaata
     2641 ttgtttttcc tggcgaagat atcacaatcc gccttgaagg aataggcttt cataaagcaa
     2701 aatttttatt tgaagaaatt aaagacctgc gtcaggatac agtagtaaaa acggacaatg
     2761 ccgtagaata tcgattgaaa atacccgcag atattagtaa aaagaaaata ttcatatata
     2821 atgacaacga aaatacaggt cgttttcttc aggtaaagga atatcaggag cccaggcgta
     2881 tggattttat cctggtagat tatggcaacg gcccaaaggc tgttgagcaa tatgccgggc
     2941 cagaactata cgaaaaaaca ataaaaaaca tcatcatctc ttttgacccc gataaaatag
     3001 atagcattaa caagttttat ggaaaacaat acgttgatat atctgtaaaa atcacaggaa
     3061 gtaaaaatga gctattagag ttgactacac ttccacaaat ggtaatatgc ccggggccaa
     3121 aatctgccag gaatatatac tatgacaaac gcgatgcaac caatgatgtt tatagcctaa
     3181 acaccttatt aggccaaaaa acatatgatc tggacgaatg ggccaaaatt aaaattacct
     3241 ttcggcatag taaagaaaaa tacaattctc ccggattaga aaggaacgta gaaataatcc
     3301 tccaacgaaa aataaagttt gatattgatg tgtcgtttcc cagcttgctt acaaaagtag
     3361 tgaaagacaa taattggcaa aacgggcttg gggtaagtat ggccatgata gcccaattca
     3421 gcttttacca aaaagacaaa atagcccgac tgatgcctta caaggtcggg gtggggtttc
     3481 tggccctcaa tgcattcaat ttcagcgaaa acattacccg tgaccttggc atcgtaatcc
     3541 ttggcagtgt atatccaacg cgcagagata caaaattttc atttccgctc tatgcaggag
     3601 gaggctattt gcttaaccgt aaaagctggt tttggctttt tggcccgggt atcagggttt
     3661 cattttaagc taaaacaata tataccttgc aatgtcgttt agttaagtat taaaaaaaac
     3721 gccgacaggt ctgaaagacg ctgtcgggtt agtttcacat ctgccctgac agcgttcgca
     3781 gaacctttca gagccttaac taaacgacat tgatatacct tgtcagttta tcaaacagga
     3841 taagcgagaa aagtttattt ttgcaaaaaa tttttaatat gtcagaaaat gtaatccttt
     3901 ctaaactgga aggagtccgc ttgaaatttg aagaagtaag ccagcaaatt accgatcctg
     3961 cggtaatagc tgatatgaaa cgtttcgtaa aactcaacaa agaatataga gaactggagc
     4021 caattgtagc cgcatgtaac gactacaaaa ataagcttag caatatagaa accaataagg
     4081 ctattttggc agaagagaaa gacgaagaac ttagagaaat ggccaaaatg gaacttgaag
     4141 aacttgaaaa agagcttccg gaacttgaag aaaaaataaa actacttctg ttgccggcag
     4201 accctgaaga ttcaaaaaat gctattcttg aaatccgtgc tggtactggt ggagacgaag
     4261 ccagtatttt tgcgggcgat ttgtaccgta tgtacaccag gtactgcgaa agcaaagggt
     4321 ggaaaatttc agtaaccagt atttcagaag gcactgttgg aggtttcaaa gaaatagtta
     4381 tggaagttac aggcgaaggc gtttatggaa cacttaaata cgagtctggc gtgcatcgtg
     4441 tacaacgtat ccccaaaacc gaaacgcaag gccgtgtgca tacttcagct gccacagtag
     4501 ctgtattgcc tgaagctgat gagtttgacg ttgagctcaa catgaacgat atacgtaaag
     4561 ataccttctg ttcatcaggt cctggcggac agtctgtaaa tactacatat tctgcaatac
     4621 gtttgacaca catcccttca ggtattgtcg ttcagtgcca ggacgaaaaa tcgcaaatca
     4681 agaactttga aaaagccttg gtggaactcc gtacacgttt gtacaacctc gaatatcaaa
     4741 agtttattga cgaaattgcc aaaaagcgta aaactatggt ttcgaccggc gaccgctcag
     4801 caaaaatcag gacttacaac tatccacaag gacgagtaac cgaccaccgc atcaattata
     4861 cggtttataa cctcccaagc attatggatg gcaacatcca ggagtttata gaccagctac
     4921 aaatggccga aaacgctgaa cgtttgaaag aaagcgaaat ctaatttaat tagctaattc
     4981 gagaatttga aaatttgaaa atgagtcaat ttgaaaatga aattctcaat tggctaattt
     5041 gttaattacc taatttgcta attgatataa aatgaattac cttcaactat ttgagaatat
     5101 taagaaaaag aaaagttttc tttgtgttgg ccttgacact gacatcaata agattccggc
     5161 atttttgaaa agcaatgaag atgcggtgtt tgaattcaat aaacaaatta ttgatgcaac
     5221 agccgaatac acggtagctt acaagcctaa ccttgctttt tacgaaacaa caggcgttgc
     5281 aggatgggtt agccttgaaa agacagtaaa atatatcaaa caaaattacc cgaatatctt
     5341 tctcattgca gatgccaaac gtggcgacat tggaaacacg tcaaaaatgt acgcccatgc
     5401 ttttctgcaa gggttggact ttgattccat cacagttgcc ccatacatgg gaaaagactc
     5461 ggtaactcca ttcctcgaat acaaggacaa atgggtgata ttgctcgcgc ttacctctaa
     5521 tgaaggctct tttgactttc aattaatcaa aaatgcagaa acaggagacc ttttgtacca
     5581 agaagtgctt aaaaagtcgc aacaatgggg aaataaagaa aatatgatgt atgttgtagg
     5641 tgctacccgt gccgagatgc ttgccgatat ccgaaagata gtaccagacc actttttatt
     5701 agtaccagga gttggagccc agggcggtag ccttgccgaa gtagcaaagc atgggatgaa
     5761 caaccattgc ggactgctgg taaataattc ccgtggcatt atttacgccg atacttcaga
     5821 aaactttgcc aaagtagcag gagcaaaggc caaagagatg caacttgaga tggaaacatt
     5881 actggcaaag tatttataaa aagaactaag ttttataaca gcatttatgg ataataaaac
     5941 acctctttca gaattaaact tccgcatgga gcggtttaag caaaacctga ccgctgcatc
     6001 tcccaactgg caatatgctg ccatattcag caaaattaac attctgtatt ttacaggaac
     6061 catgcaggaa gggatgttgt ttatcccccg aaatgaagat gctacctact gggtacgtcg
     6121 tagttatgac agggccattg atgaatcgct gtttccatct attaagccaa tggacagctt
     6181 taaagatgca ggttctgctt accaattaac agacaaaacg gtttatgtag aaaccgaatt
     6241 tcttccaata gcttttgtac aaagatttaa gaaatatttt ccttttgaaa actttctgtc
     6301 attagatttc caggtagcta tgacaagggc tgttaaaagc caatatgaac ttgactttat
     6361 gatagaatcc gggaagcgtc ataaggttat tcttgaagac cgggttccgg gcatattacg
     6421 tgaggggatg aacgaagtag agcttaccgg cgagctttac aagatgatgc tcgaagaagg
     6481 gcaccatggc gtagcccgtt tcagcatgtt cgatacagaa gccggaattg ggcatattgc
     6541 ctttggcgaa agctccatat accctacaag ttttaatggc ccaggcggaa actacgggat
     6601 gagcgcagct gttccccatg taggaagcta taccaacagg ttgaaaaaag gacagcttgc
     6661 atttattgat attggatgcg gtgtaaacgg ttatcatacc gacaaaacta tggtttacgc
     6721 ttatgggcaa gatgtatcac ccgaagtaag agctattcac aatcaatgtg tggatattca
     6781 aaaccgaata gctgaacgct taaagccagg cgaaatacct tcaaagattt ttgaagatat
     6841 catgggcagt ctgagccctg aattcatgaa aaactttatg ggctttggct cacgacaagt
     6901 aaagttcctg ggtcatggaa taggactggt tattgatgaa ttccctgtta ttgctaaagg
     6961 ttttgacatg cctctaactg aagggatggt atttgctgta gagcccaaaa aaggtattgc
     7021 aggaatagga atggtaggca ttgagaatac ctttatggta gggaaagaag gtggcatttg
     7081 tattaccggg acacatccgg ggttgatggt agtaaaataa actaatgccc tctgtgcttt
     7141 tctttacgtt ttatctgttt tagctcaaat tttcgttcta aatccttttc tttttcttgc
     7201 ctggaaaatt ttttcctttc ctctttttga atagactggt gttctttaag cattgtttgc
     7261 gctttggtga atgtgtattt atattttgaa gtttcttgaa tgtgctttgc ttttcgcatc
     7321 tggagtttgt aattggctct tttaaaattc ccatgttttt gttgggttat taaaggcaat
     7381 gtaaaccgaa gattatcaat gttaatattt tcgagaaagg cagccagctc agcacctgta
     7441 ggttcagaag cgccaattat ctcgcgggct acagcatatc catattcgtc ctctttttca
     7501 aaaaggacaa tccagaatgg ctcttcaaac aatacagata tttcgccctt cgaatacatt
     7561 acagtaaagc cgggtaaaac ttagccataa ggtataaccc ctcatcacca gcaataactt
     7621 tgtgcttcgt atcatttgga attatagcaa tatccccagg tttatacact acccttttgg
     7681 tatcgataaa acagtcggca gtaccaacaa taacctcatg gatttcagtt tttccggcat
     7741 gtatatgggt atctaaaacg caaccaggct caaccttaac aatatgggca ctaaattgtc
     7801 cctgcgtttc tgcgccagtg ataagatgct ttaaatatac ccctttgaaa gctggatgag
     7861 cattccattg cttgttgtca ttgcgttctg ttgtgttaat agtgtacaac ctgccatttt
     7921 caatgttttt aattgtttcc ataacttttg ttttaaattc aaaacaaaag taagagggtt
     7981 gaaaatgaaa ctattgtaca tttttgctct tttacaaatg aagaatattc gtgcctttct
     8041 gaaaatcaag tggagaaacc ccataatact tagtaaaatg gcgcacaaaa tggctgctgt
     8101 cataaaagcc agtcataata gcaatatcgg caatagacat cttacgttcg agcaaattac
     8161 gggcagcccg taaacgatta tcaagcaaat actgatgcgg agcaagccct gttagtttct
     8221 tgaacaaacg cgaaaagtga aacatgctta agaatgcttt gtccgccaat tcttcaagag
     8281 aaataggaac atcaatctga ttatctaaaa tatagcggat tcgttttaac ctgtcgtctt
     8341 catgattaat tacgttcctt gcatcaatac aaaattgtaa acttcttatt atttcttcga
     8401 tataaaagtt tacattttca atttccttgt cattgatgag actgttaaat aagagagaaa
     8461 gattttcagc aataacagcg ttttcaacaa tattaaaaaa ataaagcctg tcttgagtgt
     8521 aaaacttttt aagatactct aaagatatag acaaaacaat ataatcatga cccgcatcat
     8581 tggtttcgct aatactatga ggctcttctg aattaataat gaatatatcg ccttctgcaa
     8641 caacatattt tgattgattg attgtaagta tcctggaacc actgacaacc ataccaacag
     8701 ataatgacaa atgcctgtgc accggaaatt ttttagaaac accatgccca caaacaatgc
     8761 taatggcttt atcaatactt ttgtaatatt tgattttcga catgtttcaa atttagtaaa
     8821 tcgttgaaaa tgaattacca aaatattaaa agaccactcg aaattaatct ttcatacttt
     8881 tcaacggaaa atctttaagc cggataaact tatacccttg ttctctcaag gcaggaatga
     8941 tgattcgtaa agcttcgcac gtattccatt ttgggtggtt gaaatgcata atgacaatag
     9001 ccccaggctt ggcatgtttt atgacatttt gagctataat agtatcagga gtattggcca
     9061 cggcatcccc cgaaagaact tcataactaa ctacagttat tccgagcttt gtagccatac
     9121 gagcacaact ttcatcaata aatgcagtag ctgaacgata aaagcgtggt tttttaccaa
     9181 taatagcttc tattttacga gcattagctt ctatttcgtc aaaagcttct gaaggattgg
     9241 ctgtcccttt tattccatat tcgctttcgc cacaagcaga gcagggtttg tggttcaatc
     9301 catggttctc aatttcaaaa aggctgtcct gactaagtgc aacaagcgct ttgaagtgct
     9361 tgtcaatcca cctgccagta acaaaaagtg ttgcagggac attttcttta cgaagaagct
     9421 gaattaattc ataatcgtaa cctttgcctt tttcttttcc gcatgcatca aaggtaaaag
     9481 caatcagttt ctgcttagta gaaatttttt catcaacgcc tttaacaaac tcgccccaat
     9541 gaccaggctt tgcatgcttg aattctttta caaccttctt tttaagattg gtataagatg
     9601 tatctgcata gagttttttc aaaaaaacat catttttgaa ttgcccggcc atttgacaat
     9661 aggcaatgga gcaaaaaaat agtgaaatag ctgaaaacaa aaaagatttc attgtaaccc
     9721 attaaaaaaa aaggttttac ctataaagta aaacctcaac aaaatattta ataagacttt
     9781 taatcattat cgccactatc agtttcatag cctttcatga ttccacgttt agaattgcgg
     9841 ataaacatca caatttcatc acgttcagga atagcaggaa ggtttgtttc aatatattca
     9901 agtgcttgag agtggttcat atttctcaga tagatacatc ggtacacttc ctggataata
     9961 ttgatagtct catttgaaaa ctggcgcctg cgaaggccaa gactattaag accaacataa
    10021 gcaagcggtt cgcgggcagc ttttacataa ggcggaacat cttttcgaac cagagagcca
    10081 ccactaataa aagcatgtgc cccaattttc acgaactggt gcacaacagt tgaacctccg
    10141 gtaattaccc aatcgccaat ttctacttca ccagccaggc cagtatatcc ggcaagaata
    10201 acattgttac caatcagaca atcgtgggct acgtgtacat agctcatcac aaggcaatta
    10261 ttgccaatca cagttttgtt tttagcagct gtgccacgat taacagtaac atactcacga
    10321 atagtagtat tgtcgcctat ttcaacagta gtatattctc cacggaactt caaatcttga
    10381 ggaaggccag caatagaagc gcaagaatca atcttacaat ttttaccaat acgcgcacca
    10441 ttacggataa cagctccggc accaatccag gtcccgtcac caataataac gtctttttcg
    10501 attgtagcaa aaggctcaat gataacgttc tgccctattt gagcctcagg atgaacattt
    10561 gtaagaggat gaaaactcat attttagatt tttggcaaat gtatattttt tattttgttt
    10621 taacaatctg agcgcaaagt tctgcttctg taaccacagc attgccaaca aaagcctgtg
    10681 cccacatggt aacgatacca cgacgtatcg gttcaataag ctccaatttg aaaatcagcg
    10741 tatcacctgg gacaactttc tttttaaatt ttactttgtc aattttgaga aaatatgtac
    10801 tgtaagtttc agggtcttca actgtattta aagctaataa cccaccacat tgagccaaag
    10861 cttcaatgat aagcacacca ggcattactg gttctccagg aaagtggcca acaaagaaag
    10921 gttcgttcat tgttacattc ttgataccaa caatcatctg gtctgtacgc tcaatgattt
    10981 tatcaaccaa taggaaaggc ggcctgtgtg gcaatatttt cattatttgg ttaatatcaa
    11041 gcaatgggtt ttcagccgga ttatattttg gaacagctgg ttttaagctt tcttttttta
    11101 ttatttttcg aagaagttta gcaaattcag tatttgcatg gtgcccaggg cgtgaagcaa
    11161 ttatcctggc tttgattggc atacctacta acgttaaatc accaaccatg tcgagcaatt
    11221 tgtgacgagc tggttcgttg cggaaacgca gttcgatgtt gttcaacacc ccttcaggct
    11281 gaactgaaaa cttaggttta ttgagcaatg cagataaatg atcaagttcc tcttgctcga
    11341 cttttctatc aacgattaca atagcattgt caatgtcgcc acctttaata aggttatgtt
    11401 tggccagtaa ttcaagctca tgcaagaaaa cgaaagtacg acaaggtgca atttctttag
    11461 caaattcttt caacgaattc attgaagcat actgattgcc aagcactttt gaattatagt
    11521 cgatttgaac agtaatgctg aaatggtcat cagggaaagc aacaatttca ataccctttt
    11581 caacatcatg aaattcaatt ctttccttta actcaaaaaa ttttttttct gcttctaact
    11641 ctacaatacc aaccttttca ataccttcaa cataaggttt ggcactacca tctaaaattg
    11701 ggacttcagg ggcatcaatt tcaatcaaag cattgtcaac acctaaacct gtgagtgcag
    11761 caagcaaatg ttcaagtgta ctaatacgta caccattttc atcaagtgtt gtgccacgtg
    11821 aagtgtcaat tacattatct acaatagcgt gtacaatagg attttcatcc aaatcaatac
    11881 gcataaacct tataccatga tttgctgccg ccggtttgat atacatgtta accataaggc
    11941 ctgtatgaag tcctttgcct gaaaaatgca cctctgcggc cagcgttttc tgtttttctg
    12001 ccataatgga gtatgatttt aaatattttt ctattgattt ttattcttta tctctttaat
    12061 gtcgcgctct acctgattta actgagcgtt tagcttaggc agattgcgga aaagaacata
    12121 acatttcatg tattctttaa agtcaaatgc aggagcacca atcatagttg cgtcttcttt
    12181 cgtaatggaa gcattaacac ccgattgtgc gccaagttta actccatcgg caacagttat
    12241 atgaccagcc aggccgacct ggccgccaaa catacaattc ttgccaactt ttgttgaacc
    12301 agcaacgcct gtctgagctg caaatacagt attctcgcct atttcaacat tatgagctat
    12361 ctgaataagg ttgtcaagct taacgccttt acgaataatg gtcgaaccca tagtagccct
    12421 gtctattgta gtattagcgc ctatttcaac atagtcttcg ataacaacat tgccaatctg
    12481 aggaaccttc ttgtaattgt tttctgaatt tggagcaaaa ccaaagccat cggcccctat
    12541 caccgctccg gcatggataa cgcagttagc gcctatctta caatcggcat aaatcttcac
    12601 tccaggataa ataacagtgt tttcgccaat agtaacgttc tcgccaatga aagcctgagg
    12661 ataaatctta gcattgttgc caatttttac attgttgtct atgtaagcaa aagccccaat
    12721 atacaggtct gtaccaaggg ttgcagattc actgatatag caattgcttt ctattccaga
    12781 cttttgaggc tttgcctgtt gatacatctc caaaagagcg gcaaaacact gataagcatc
    12841 gtcaacctta attagggtgg ctgatacagg cccttcaatc tgtaatgttt tatttacaag
    12901 cactattgaa gcctttgttt catacaaata cttattgtac ttgggattag ccagaaaagc
    12961 cattgttccc ggccgtcctt cttcaatctt agcaatatct gaaacctcaa cgtccggatt
    13021 tccgacgact tcccctttta aaaagcttgc tatagcttgt gctgtaaatt tcatattgtc
    13081 aatattatgt tttgttttac tttttcaggc ttttgaccaa taaaactatg cttgtctgtt
    13141 atttaaataa gccaacaaag ataatatctt ttatatttta ctgattattt cttttggata
    13201 acaaagaaaa tgcttggtca cgacctttgt aagcacctgc atattgagca tatccgaagc
    13261 ttcagcaata tcaactactt tgccgttgtt atataatatt cctatgttgt gttcgcctgt
    13321 ttcgtatata ttgctggtaa tagaatcggc aaaaaccata aatgaagcct cttttgatga
    13381 aagtgaaaaa tgcttttctg ttttttcaat catttcagca atacgttcag cagagaaagg
    13441 ctgattttgg agttcaattt tatacaattg ccggctaatc attttctgac aaagggttga
    13501 caatatttta tcatcatgtt ccatccatac tttggtggct gagataatat ctgtatcatc
    13561 aagccttaca aaatgctgga gcatcgtatt taaatctttc tcgtcagcag cattgatgaa
    13621 gcttctgtca aattttttga acaaaaagta ttctaaagca ggaggtgcaa ataatttatc
    13681 accctgtaag ctcagttctt tagctcgttt caaaatattt atcaacaaat tctcagcaga
    13741 caaaaccgtt ttatgaagat acacctgcca atacatcaac cggcgggcaa tcaaaaactt
    13801 ttcgatagaa taaatccctt tagcttcaac caccaacttg tcatccacta cgttaagcat
    13861 cttgataatg cggtctgagc caacaactcc ttctgaaacc cctgtaaaaa aactatctcg
    13921 gcgcaggtaa tcgagcctgt ccatatccag ctggccagat acaagctgat gcagaaacct
    13981 cttatgatat ttatcctgaa aaatggcaat ggccaatgta agcctattgt tgaattgggc
    14041 atttaatcca gccataagca attcagagat actttcatgc gtaaagcctt caataatgcc
    14101 aaattccaaa gcatgtgaaa aaggtccgtg tccaatgtca tgcagcaaaa tagcaatatt
    14161 aacagcctca gcttcttcat cagtaatttc atggccttta gaacgaatta cttctatagc
    14221 ctggttcatc aggaagtaag cgcccaatac gtgttgaaaa cgagtatgca tagcccccgg
    14281 atatactaag ttggtaagcc ccagctgacg tatcctgcgc agacgctgca cccatggatg
    14341 ttctatcaaa tcgaaaatta aatcattatt gatagaaata aacccataaa cagggtcatt
    14401 gacaattttt cgcttatttg tgttcaaaag agaaattttt acaaaagtaa gattttgtaa
    14461 ttaacatgtt tttatgaaaa attaataata aggttaaaaa tgatattaaa ccccaattaa
    14521 tcatcaggag gccatttctt gccgaactat atgatttagt tgagttaacc ttcccgttat
    14581 aaacgggaca agctgattta gtaattgtct gatgttattt attaaattgc aggataacaa
    14641 tttattaatc gattaagtat taatttgggt tttaaaagat aatgaacgaa taaaaaggaa
    14701 tcgtagctaa agattagcga ggtttaataa tcacagcgtt tgccaatttt ttttacaaag
    14761 aacgcaaagt attactaatc attaatactc tgcgttcttt attttctttg tttcgccgca
    14821 attaatttgc agttaagtat tctttatcag caattccaag gtttaagctg cttaaagaaa
    14881 tcgtttcctt tatcatcgac aagaataaac gccgggaagt ccacaacttc tatcttccaa
    14941 atggcttcca ttccaagctc cggatattca agcaattcta ccttggtgat attttgttgg
    15001 gcaagaatag ctgcaggccc accaatactg ccaagataaa agcctccaaa cttcttacat
    15061 gcctcagtta cttcagggct acggtttcct ttagcaatca taatcatact tcctccattt
    15121 tcttggaatt tagccacata cgaatccata cgcccggctg ttgttggccc aaacgaacca
    15181 gatgccattc cagcaggggt tttagcagga ccagcgtaat atatcgggtg gtctttcaaa
    15241 tactgaggaa gcccctctcc tttctcgaga cgttcgttga gcttggcatg agcaatatcg
    15301 cgccccacga taatagtccc gttcaatgaa agtctggtag ctacaggata tttggtaagt
    15361 tctgccaaaa cttctttcat tggacggttc aggttaattt tcaccgcttc gccttctcca
    15421 acatttctca acctttcagg gataaatcgc cccggattgt cctccagttt ttcaacccaa
    15481 ataccatctt tgttaatctt ggctttaata ttgcggtctg cagaacatga aacacccata
    15541 ccaacaggac aagaagctcc atgtcggggc aaacgtataa tacgcacatc atgggcaaaa
    15601 tatttaccac caaattgagc gcccatacca aactgctgag ctgcttttaa tattttttgt
    15661 tcgagctcta catcgcggaa agcacggcca tgttcgttgc cggtagtagg caaagtatcc
    15721 aaatatttgg tagatgccag ttttattgtt tttaaacatg cctctgcaga agtgcctcca
    15781 ataacgaata ccaaatggta tggaggacag gctgcagtac caagcgtttt cattttttca
    15841 ataagaaagt tttcaagctt cccgggagtt aacaaggctt ttgtttcctg ataaaggtat
    15901 gttttattgg cagagcctcc ccctttagta acaaaaagga acttgtattc atcgcctttt
    15961 tcacagtaaa tgtctatttg ggcaggtaag tttgtcccgg tattcttttc gttatacata
    16021 tctaaagcca cagtctgcga atagcgaagg ttttcttcag tatatgtttt atatacccca
    16081 agagatatgg cctcagcgtc attaccatcg gtaaacacaa gatgtccttt ctttgccact
    16141 acagtagcag ttccagtatc ctgacaaaac ggcaattggc ctttagcaga tacctccgca
    16201 ttacgtagca tagtcaatgc tacatattta tcgttctcac ttgcctgtgg gtcgtcaagt
    16261 atagcagcaa cctgctgttg atgttcagtg cgcaagagaa atgaacaatc gcgcagtgct
    16321 gcattggtaa ccattgtaag cgcttcagga ttaacacaaa gcacttcttt cccatcaaat
    16381 tgttttacag atacatgctc tttgctaagc aagtaatatt cggtattgtc tttttccaat
    16441 ggaaaaagtt cctgataata aaagggaggt gttggcatga tgattactaa ttactaatta
    16501 ctaattacta atttcggata acaatgaaat attattttat taaaatatct gcatttgatg
    16561 gaggttgagc aagtactgct ttatattttc caacaattat aggcctttga aggagctttg
    16621 gattgtcaat gataatctga acccactcat cgtctgagaa attgcgacct ttaagctcta
    16681 ttttgtattc aggttcttgt gtacgcacca attctacagg ttttttgccc gatttgagaa
    16741 atatttcttt caactcgtca gaagaaatgc ctttttcaag atacttaaca acctcaaaat
    16801 ccactccttt ctctttcagg taattcaatc cctcgcaact ctttttgcaa cgcggattgt
    16861 gatatatttt aagtttttcc ataaacaaaa ttttactgtc ccccatattc catcaaaaat
    16921 gctttgatga aaccatctaa tccgccatct aacacatctt gagcattgga agtttcataa
    16981 tcagtacgca aatcttttac cagcttatac ggatgaagta catagttacg aatctgcgac
    17041 ccccactcta ttttcatctt tttcccttca acttctgcct gtttttcgcg gcgtttttgc
    17101 agttctaatt cgtacaacat agaacgtaaa atacgcatgg cgttttcttt attcttcaac
    17161 tgtgagcggt cttcctggtt ttcaatgaca attccagtcg gggcatgtcg caaacgcact
    17221 gctgtttcaa ctttgtttac attctgcccg ccggcacccg aagaacgata tgtttcccag
    17281 gttatatctg cgggattaat aacaatttca atggtatcat ccaccaaagg cgatacaaat
    17341 actgatgcaa aagacgtgtg acggcgtgca tttgcatcaa acggcgagat tcggactaaa
    17401 cgatgtacac cactctcccc ttttagaaat ccgtaggcaa attcgccatc aaattctatg
    17461 gttgcagatt taataccagc ttcgtctcct ttcaggtaat caacctcttt catcttgtaa
    17521 ccatgacgct caccatagcg caggtacata cgtaaaagca tgtctgccca atcttggctt
    17581 tcggtaccgc ccgctccagc atttatcttg aggattgcac caaacttgtc ttcttcattg
    17641 cggagcatat tgcgggtttc taaatcttct acaatcttaa gcgcttgggc atactgggca
    17701 tcaacgtctg attcttcggc ttggccatct ttggcaaagt cgaacattac ttcaagatcc
    17761 tcaacagcac tttttacctt ggcataatgg tctgtccatt gcttaataga cgacactttc
    17821 ttgagttgtg cttcagcttc tttcggatcg tcccagaaat tgggcgcgtg agtcttttgt
    17881 tcctcttctt caagttcaat caagcgacgt tcgatgtcaa agatacctcc taagggcatc
    17941 ttcacgctct ttcaaatcct ttatctggtc tgttgtaatc attgaatatt tttttgcaaa
    18001 ggtaaaactt atttgcctga tgcagcaatg ctaaattatt tactttcctt cccctttggc
    18061 agctgcatat tcagttggcg ttaaaccaaa ctgggcaata aaacacttac taaaatattt
    18121 tgggtcatta aaaccaatca tgctgcatag ttcagtaata gggatcttat ttttttccaa
    18181 aataagcgca gcctttttca aacgtacact tctgataaac tctacgatat tcatccctgt
    18241 caatgacttt atttttctaa acaacactga atgactcata gccatatcgt gcgtcaactc
    18301 tttaatacca taatcaggat tagctaaatt ctgctctaca acttccatca gctttttcag
    18361 aaaccgttcg tcaagagaag taactgcaat atctttaggc tgaaggatca actgcttgtt
    18421 aatactgttt tgtatgcgct tgcgcgagtc aagaatactg ttaatctgag cctttaagac
    18481 atcaacacta aatggtttct gaataaacac ttctgcacca gattcaagcg cctcaatgtt
    18541 tgcatcgctc gcagaacgtg cagtaataac aataacagga atatggctcg tatgaatatc
    18601 gctttttagc ttcttacata atgtcaagcc atccatcacc ggcatcatca ggtcggtaat
    18661 aatcaaatca gggttgatgc tcaaggctgt tttgaaggca tcttcaccat taggggctgt
    18721 tacaatctta aatttctggc taagctttga tgatatgtaa tccaaaagtt caagattatc
    18781 ttctgccagc aataatacat tttctacttg taaagatgct tgtacttttt catttaatga
    18841 gttggccata cgctcaagat attgttgact agaatcatta gcaggttctt tttgagcacc
    18901 actcttatct tcaaaatatt ctgggctttc tatatgcaat aaatgtctat aaccttgttt
    18961 tatagtaatt gtaaaacaac tacctttacc aggttcactc tctacttgaa tatttccgcc
    19021 atgcattaac ataaactccc tggcaataga caagccaacg ccactaccag gaataaactg
    19081 ccctttttca ttgtgaaccc tataaaaacg gtcgaaaatt ttatctaagt gttctgatga
    19141 aatgccacaa ccgttatcta tgactttaat caaaatattt ggcaatgtct gcccaccatc
    19201 ttcctgttca ttctctgttg gttgctcata gcaaagctca agagtaatca aggagttgtc
    19261 cggcgaatat ttaaaagcat tggacaagag gttaaacatc accgtcagaa ttttctccct
    19321 gtctatccat gccatcactt ctgaaggttt atgaatatat tgaagattga tattacggct
    19381 tagtgcatat tggtcaaaaa ccgtatagac atcattcagc aacaacacaa ggttttcgta
    19441 cgacaaagta agcttaacct gccctgcatc tatctttctg aagtcgagta actggtttac
    19501 catgtgcagt aagcgttgaa catttttata tacaactgaa agctgagctt tgactttgtt
    19561 atcaacgctt tcatcatgta ttaaatcttc aataggacta ctaataagag taagcggcgt
    19621 tctgagttca tgagatatgt tggtataaaa ttgaagtttc aatttggtca attcttcaat
    19681 tttgtctttt tctaagctat ctataagaag ctgatttttc tttttcacag ctatcaatga
    19741 atacttctta aataaaatat aaagcactac caaaaccata aaatacaata gaaatgccgg
    19801 ccatgtcctc cacaaagggg gcagaacaac aattttcatt gaggtatatt tttcagacca
    19861 aacaccatcg ctatttgtag cttttacttg caactcataa tcgccaggcg acaaattggt
    19921 ataggttacg ttgcgtatgc ggtttgaaat cggctgccac tgctcgttaa agcctaacaa
    19981 cctgtattga aattgaattt tttcgggtat tgtaaagtta acagctgtaa aaccaatgct
    20041 aaattcgcgc tctgaagcct ttaattcaat tgtctttgtg gccaatattg aagaattgag
    20101 caatactcga ctgttgaatg attgattgat agggacatct actccattaa ttttcaaact
    20161 ggtaaaaaca acgttggttt tggaagtgtc aaggtctatt tctgaaggat taaagtaggt
    20221 caaacctgct attcctccaa agtacatgcg ccctttattg tctttaaagt aagcattttg
    20281 cctaaattcg tttgactgta ggccatcatt tacatcaaag tttaccaaat aatgtttttt
    20341 aacaaggtat ttagaaatac ctttattggt acttaaccat atattgttgt cattatccac
    20401 caatatcccg tacacaacgc tattaggcag accatttttt tcgttccaaa tcgtaaattc
    20461 aggttcaaca ttatcgttga gcgcttttaa agataattta ttaagtcctg caactgttcc
    20521 tatccatata ttcttttctt tatcttcagt tacaacatat acatcattgt gagctaaaga
    20581 tttcttcttg tttttatcat gatagtagtg tttgtacctg gctttatcag gcgctgcaga
    20641 agggttgagc aacctgtgta gcccattacc tttagtgcca atccaaagct tcttctcact
    20701 atcttcaaaa agattgaaaa tgatatcgcc ttcggtcgaa aactcatcag aaagattgag
    20761 cttgtagtgt tccaatgtag ctttagcctt attatatctg tataaaccct ggccccatgt
    20821 gcctaaccaa atgtacccat aactgtcttc aacaatagtg aaaacgtaac caaatattga
    20881 cagggtcttt agttgttgtg ttattgaatt aataatgttt atagaatttt gagcagaccc
    20941 aaaccacaca gagccatttt tatcttgata taaactaata acctgtttat ctttcccgga
    21001 aagattgcgg ttccaatgtt ctatgttcag aaacatccca cttaaagggt caaaacttaa
    21061 tttatcaatg ccgcctccat cagtagcaac aaaaacagaa ttaggctgcg agctatgtat
    21121 tgctgttaca aaacttgtac ttaacgcatt ctgggttgat ccaatatttt tgatggtatg
    21181 aaatttcttt cggctcaggt tcaaccagtt taaaccttta tattcagaac ctatccacaa
    21241 aatattagta tttccaataa aaagacaatt caaccgctcg tcagatatgg cattttcaga
    21301 agtattgtca gccttataat aagttattgc accatcttta gctattttag ccaagccctg
    21361 tctcgaagct acccaaatat tgcctttttt atcttctgta attgcggtaa ctcttataaa
    21421 tgatatatta ttctgatggg taacctgaac tttcccttgt atattctgta atttatacaa
    21481 accgttttcc agagtcccaa tccacagaat tccattcttt tgttggtgaa gagctattac
    21541 agtaggaaag gccagttcag aaaaaggata caatgtagat aaggtattgc ctgtcatatc
    21601 aacataatat aaacctccca atgtacctat cagtatcttc ccatctgttg actcgcaaat
    21661 agaacgtacc tgataatttg caagttgttt atttatagta agttgcaccc tttcaaacct
    21721 aatttcggtt actgactttg aattagcagc agcacgggcc ctgtaaagcc ctatttctgt
    21781 gcctacccac atgtttccct tcgaatcacg aaaaacccgc ataatctcat tgccactgat
    21841 gtttttaaaa aactctggcc tgtttattat gttcaaaaag ctatcgcaat gaggattata
    21901 aatgtttagc ccgcctccaa aagttccaat ccataaattg cctgaagtat cagatgtaat
    21961 agctaaaatc tgattgtttg taaggctgtt cttatcctgt gggtcattct tatacacttt
    22021 tacttcgtaa ccgtcaaaac ggttcaaccc atcttgggta ccgatccaga caaatccaaa
    22081 acagtcctgc gttatttcat ttacagccga ctgcgacagc ccgtcagata tagtaaggtg
    22141 ttcaaacctg aagttatcaa aattatcatt atcagcagca tttacaataa aactaagaaa
    22201 cagaaaaaca gttataattg aaatttttcg catgacatcc atctcttttt tctttttcaa
    22261 tggtcaaagg taattatttc ttgatttaat aataaaaggc tatttgacac ttaaccaatc
    22321 atataagttt aataccatga aacacccatg ccttggccta acgcaaacac gatggtattt
    22381 atctgtgggt gcatgctatc attccaacaa aaattaaaaa taaatatgat gaaatagcta
    22441 tgtttaaaat aaaaaaagca aagtcatcta taactttgct ttctttattg tcaatttgaa
    22501 ccttctctaa tgcctcatct acttaacttt cgtaccagga tattcttgta atgctttttt
    22561 taaacactct acagcattag tcagatcgtt gatattcaaa acgtatgcaa ttcgcacttc
    22621 agatttccca agtcccggag ttgcataaaa ccctgcagcc ggtgccaaca ttactgtttg
    22681 gttgttatat tcaaagtctt tcaataacca cgtagcaaac ttttctgcat catccacagg
    22741 caagtgcgca atggtataaa atgctccctt gggcagtgga caaaataccc ctttgatttt
    22801 gttcaggcgc tcaaccaaaa agttacgcct ggcaagatac tcattataag caacttcaaa
    22861 atactcagca ggagtatcaa ttgcagcttc accagccatc tgtccaaacg atgggggact
    22921 taaacgagcc atggcataac gcattgcaat attaacaacc tctttgttgc gggttaccat
    22981 ggccccaatc ctaacaccgc aagcactata acgttttgat actgaatcta tcaaaatggt
    23041 gttttgctcc attccttcca gctccataac cgagaaaaat ttttccccgg tataacaaaa
    23101 ctccctgtag acctcgtctg cataaagata taaatcatat tttttaacaa tacgacggag
    23161 ttcctcaagg tcgtcacgcg aatacaagca acccgtaggg ttattcgggt tactcaacat
    23221 gatacccctt gtgcgggcag taattacctt ctcaaattcc tcaacaggag gcaatgaaaa
    23281 gccattctga atggatgatg ttacaggcac tacatgtaca ccaacagccg aagcaaaact
    23341 attataattg gcataaaacg gctcaggaat aattatttca tctcccgggt ccatagtact
    23401 aagaaatgca aagttgatag cttctgaacc tcctgtagtt attaacacat cgttataatc
    23461 aacattaatt ccctgcttac aataatactg ggctaatttt tttctgtaac tttcattccc
    23521 agccgaatgg ctgtattcta caacccttcc tacatatgac tttacagctt ccaacatcaa
    23581 tggtgctgtt ggtatatcgg gctgcccgat attcaaatga tatactttta taccctttcg
    23641 tttagcttct tcagcatagg gcataagttt cctgatggga gactggggca tatcaatacc
    23701 ccgttgcgat atttttggca tgattctttc ttgtaaaatt gatactactt cttcccctct
    23761 gttttaggta ccacggtacc tttaatagta agagttacag gagaattggc cgcattagaa
    23821 tacactgtga tagttttatt aaaacttgtt accacgtggg tattgtaatg aattttaatt
    23881 actccgctcc tgtttttttt taccgggtcg cgtgtccatt caggaacagt acatccgcat
    23941 gaagtatgca cattatctat caccagaggt tcttttcctg tgtttctgaa cacaaacgcg
    24001 tactccgcca cactgttttg ctcgatagtc ccaaagtcgt gctctgtatc ggcaaaagca
    24061 atgatcgccg cctttttgtt gccttcatta cttgcatgag tacaactgag aaaagtcgcg
    24121 actgcgattg ttacggttac taaaaagaat ctcttcatgt tttacaatta cgttttatca
    24181 atttgaaaga caaagatata tttttttaca tggcataaaa aaatgatttt ttcaatctat
    24241 ggccattatt aattgcaatt ttcacaaata taaagtgcaa ctctttaata attaaattat
    24301 taaaaagttg cactttattt tagaatttag aatatcgtac ctaagaaaat ttattcgtaa
    24361 ctcgtaattc gtaactcgta attatttaaa aaagcttttt tttgcagcca ataatgtatt
    24421 ttgcaacagc gaaacacggg tcataggccc aactccacct ggtacaggag taataaatga
    24481 gcatttaggg gcaacttcat cgtaatctac atcgccttta agtctgaaac cactctttgt
    24541 ttcaggagca gggactcggg ttgttccaac atcaatgata acagcaccct ctttcaccat
    24601 tgaagccttt aaaaaaccag gtttcccaag agctgcaata ataatatcag ctttagaggt
    24661 atatgaagct atatcaggag tagcactgtg gcatacagtt acagtacaat ccccgggttg
    24721 agccttttga gaaagcaata tactcatagg ccgccctaca atattgctcc tgcctattac
    24781 aacacaattc tttcctttag tttcaacctt atacctacgc aacaactcta caattccaaa
    24841 aggagtagct gatacatatg atggaagccc tataaccgtt cttccaacgt taattggatg
    24901 aaaaccatca acatctttac gataatcaat agcctcaata atcttttgtt ctgaaatatg
    24961 cttagggaca ggcagttgga caataatacc atccacatca ctgtcattat tgagcttctg
    25021 aacagcagcc agcaattctg tctcggttac atcatcttca aaccttacta aggtggactt
    25081 aaaaccgact tcagcacagt cttttacttt gtgggcaaca taagtctcac ttccaccatc
    25141 atgaccaacc aaaatagcag ccagatgagg tattttcaaa ccctgctctt tcatttgctt
    25201 aacttcttcg gctatttcga gcttaatttg ctcagcaacc ttctttccat caattatttg
    25261 cataaacaat attttaaata tttacttcct ttatcttctt aaactcccgt attcttactt
    25321 taccttcctc aaacttccat aaaattctgc aatcttcttc tttacaacct cgcactaatc
    25381 ttcctcagcg tttccacctt ccaactcgca aaagtattgg ccagtatttg cttacgggct
    25441 tcttttacca accacagata aaaatgcaag ttatgaatag ttgcaatctg cgcacccagc
    25501 atttctttag aaaccacaag gtgccgcaaa taagctttag aatatacctg atctacataa
    25561 caatcacttt ctgcatcaat aggcgaatag tcgtctttcc atttttcgtt tcgcatattc
    25621 ataatgccat tacgggtaaa aatcatacca ttgcgcccat ttcgggtagg catcacgcaa
    25681 tcgaacatat ctatgcccaa acttattgct tccaggatgt ttgcaggtgt tccaactccc
    25741 atcagatagc gaggtttgtc tttaggcaaa atatcattga ccacctcaat catctcatac
    25801 atcatctcgg caggctcgcc aacactaagt cctccaatag cattaccttc catgccaaca
    25861 gaagcaatct tttcggctga tatttttcgc aaatctttat aaacactgcc ctgtacaata
    25921 ggaaaaaaag tctgcccata accataaaga ggttccgttt ctttaaacct ggcaactccc
    25981 ctgtctaacc actgatgagt aagttttagc gacttctcgg cataccggtg ctcgcacgga
    26041 taaggcgtgc actcatcaaa agccataatt atatcagctc caatgatacg ctgtatgtcc
    26101 accacatttt caggggtaaa caaatgtttc gacccatcta tatgcgacgc aaagtgggct
    26161 ccgtcttctt taagctttct gttttctgat aaagagaata cttggtaccc cccactgtct
    26221 gtaagtatag gtttatccca gcctataaaa cgatgtaacc ccccggcatc tttcataatc
    26281 tgcatccccg gcctcaaata aagatggtaa gtgttgccca gtatgatttg cgcccctata
    26341 tcatttacca actcgcgctg atgaacacct ttaacagaac cagctgtgcc aacaggcata
    26401 aatataggag tttctattat tccatgatct gtttcgatgg ttcctgccct ggctgcagac
    26461 tgcttatcgg tattgtgtaa tataaatttc aacgttttgc cccttattcg aaaattgctg
    26521 caaagatata tgatttaata agttgacact aatttatggt taaacaatcg ctatgcttca
    26581 tagataaaag gctccgagct tttcatcaaa cttattaaaa taactaacac aaaaaatccc
    26641 tgtttcttaa aaatcctttc tctttgtccc ttgctcctgg ttcttacttc ttgactcttt
    26701 gttcttgaca ctaaaaaaaa cgttaaaatt gtaaaaaaaa gcagttgtac gatacttaaa
    26761 acttccgtat cattgcaatg ctttaaaaaa tgacagttta aaacatgaca actttagaaa
    26821 ttaccggctt ggcaacactc tctgtattaa tactttgttc ggccatccaa tatatttttt
    26881 actggtttat tttcaccaaa cctttttcta taaaaaacaa aacatcagaa gcaagccatc
    26941 ctgtttctat cattgtctgt gcccgcaacg aagaagccaa ccttcgggaa aaccttccat
    27001 tactgctcga acaagaatat gccaattatg aagtcgttgt tgtgaacgat tgttcctcag
    27061 acgacagcga ccttgtactt aaagcccttg ctgcccaata tccccattta agatatacca
    27121 ccattaaaga aaatgaaaaa tttcagcatg gcaaaaagct ggcccttacc gtgggcataa
    27181 aagctgcaaa aaacgaatgg atgctgatga ccgatgccga ttgcaaacct tctgggccac
    27241 attggttgag cgctatggca agccaaatga ctgaagataa agctatagtg ctgggttatg
    27301 gcggttacct ttctcagcct ggcttactaa ataagcttat ccgcttcgaa acagcatgga
    27361 tagcccttca atacttctct cttgctatta taggcaatgc ttatatgggt gtaggcagaa
    27421 acttatcgta tagaaaatct cttttctttg ccaacaaagg atttgcttct catgcccgtt
    27481 tgtcgtcagg cgatgacgac ctatttatca acgaaacagc cacagcaaca aatgtaagtg
    27541 ttcagttttt acctgaagct catacataca gcaaacccaa aaaatcactt tcggaatgga
    27601 ttgaacaaaa aagcaggcat tttcaaactt acccgcaata taagttcaaa cataaatttc
    27661 tgcttaccgc tgaaactttt tcacgtgtaa cattttactt ttcgcttgca gcgttgttta
    27721 tactccaaca attcatttgg gtggcattag ctttattgtt tgtacgattg gtcagccagg
    27781 tgattgtgct aaatgtttgg ttcaaaagat taaacgaaaa aaaattattc ttactttcgc
    27841 taatatttga tttggtaatg ccatttttat actttattat ttatttgact aaaaaaatca
    27901 aacctgcccg acaatggagg taaatacaaa cctttcggat aaagctcaag cagatatggc
    27961 tcttgtacaa agagctacag ccggagacca acgtgctttt gcagaattac tttcacgtta
    28021 ccgggattct atttacttta tgctgctaaa aatggtcaac aaccaagatg acgcagaaga
    28081 tcttaccatt gaagcatttg gcaaagcctt taaaaatatt catcaatata ccccaaatta
    28141 tgccttcagt acatggctgt tcaaaattgc aaccaacaac tgtattgact ttatccgcaa
    28201 aagaaggggc ggaataacta tatccatcga ccagggattt gctaactatg aagatgacct
    28261 gccaatacac attcacgaca ttgaaccaga ccctgaggcc aatatgatca atgaacaaaa
    28321 ggtaaaagtc atgcgagaag tagtgcaaaa gctgaaacct cgttatcgta agttggtcga
    28381 aatgcgctat tttgatgaac tttcatacga agaaatagcc gaacagttag atttacccct
    28441 tggaacagta aaagcccaac tcttcagggc aagagagctg cttttcaata tcctcaaaac
    28501 ttctgaacat aagatttgat gaacattgta ctcaaatatt tccctgaatt gtccgacaat
    28561 caaaaaagac aatttgccgc actgtatgac ctttattttg actggaacgc aaaaatcaat
    28621 gtcatatcac gcaaagattt tgagcatatt tacgaaaggc atatattaca ttcattagcc
    28681 attgccaaat tctataagtt ccctgccggg acaaaaatca tggacgtagg tacaggcgga
    28741 gggtttcccg gaataccttt ggccattatg tttcctgaat gtaactttac actaatcgac
    28801 tctattgcaa agaaaataaa agttgttgat gctgttgcca atgctattga gctttctaat
    28861 gtcaggccca ttgcaatgag ggcggaaact gtgcctgaaa aattccatta cgttgtaagc
    28921 cgtgcggtaa ctactattcc cgaaatgatt tcttttataa aaaaaagttt cgctgacgaa
    28981 aatattaacc catacatcaa aaacggcctt atttacctca aaggcggtga cattgatgcc
    29041 gaaatcaagg attataaaaa ccgcacacgt gtgcatgaga tttcagaagt ctttacagaa
    29101 gcgtttttcg aaactaaaaa agtcatttac attccggcag cagcttttgc aaaataaacg
    29161 ctttcgcaaa acaccttgca ttttgaaaat gctaaacgtt tttttattaa ttttgataaa
    29221 aattcaaatc catgtacctg gataactata tggaaagtat gaagaacctt tgtcaacaaa
    29281 acaaagtaaa agctttatat gtttttggat ctgttctgac ggacaggttt aatgacaaaa
    29341 gcgatattga tttaattgtt gacattgatt ctaatgatcc tttggaatat gctgataatt
    29401 atttcaacct gaaatttgcc cttcaggatt tatttaaaag gcctgttgat ttattggaaa
    29461 acaaggctat taaaaaccct tacattcgtc aaaatattga caattctaaa catttgatat
    29521 atgcaaaatg acaagaaata cgacattaga gcttgtctaa aagatatcga attgtcaatt
    29581 gctgaaattt atcaatttct accggccaaa agaaatttcc aagagtttca aaaagactta
    29641 aaaaccagga aagctgttga acgcaacatc gagattattg gtgaagctat gggtagaatt
    29701 ttaaaagctg acccaacttt tccaattact gactctcgta aaattgtaga tacccggaac
    29761 cgtattattc atggatatga tagtgtatca gaagatatta tctggcttat tatttgcaaa
    29821 taccttccta ttcttgaaaa agagataaaa aaattcttat aacttccata aattgtgcgt
    29881 accctaccct aaaaatcttt tcatcccgca accttcttaa caatttgtat ctttgaaaaa
    29941 aataatatga tgaaaaaact gatatttcta ttattgctgg ctccaatagc attaacagcc
    30001 caaaatacag atattttacc ttacatcgaa gtcacaggca acgccgaaga tgatgttgaa
    30061 cctaataaag tagtgctttc cctatctgtt tccgagaacg aaaacataaa aaaagagagc
    30121 aacacggtag ctatggaaaa taaaatcagt gcatttttaa agtccatagg tactgaccct
    30181 gccaactttg tcgtggacaa atacaatacc aacgaacgct acaccattat ttccggcaaa
    30241 aatgtcacta tcaaaaaata ttacaagctc actatcttga atttgtcctt actggacacg
    30301 attattacca agtgcagcga agtagggatg gacaaaataa gtatcttcaa aattgaaaat
    30361 acatctattg acagtataaa caacatattg ctggcctctg ctgcaaaaaa cgcccgtcag
    30421 aaggccgatg ccatagcttc agccttaggg gtttctatca gcaacataca aattgttaat
    30481 gctaaccact tcgttgaacc aaacatagtc aatgccaata attttaaaat ggagaaaatg
    30541 ttgttctcgt ccgaagatgc cgctactggc aggacaagca gctcgcttgg cttgaacaaa
    30601 atccacctca acgcctcagt atttatcagg tttgggataa aacaataagc aggatagcca
    30661 aaagaggctt tgcttttttg gaaaagcaaa gcctcttagc cccttgttcg actattattg
    30721 acctttcagg tttttaaaat ctgaaaggta tgctgtcctt aattatactt caccggctgt
    30781 ttctcagcct cggccaatat agactgggta tgtgatacaa acgcctttgt cctttcgttg
    30841 agctcgctga tgatttccga aatcgattca atactcgacc gtacctcagc attcagctta
    30901 tctgcacttt taaactcttt cgaagtgttt tttatctttt cgttgatttg tataaaaaga
    30961 tctgctattt cagtcaggta aggctttatt ttattggcct ctatctgcga attctcagcc
    31021 agcttcttta cctcattggc cacaatagaa aaacccttgc catgttcccc agccctggca
    31081 gcctcaatag aagcatttag cgaaagcagg tctgtctttg aagctatggc attcaacgtt
    31141 tcaataactt tagaaaactc gtccagtttg ctattgttat cgtccacttc gttcaacaaa
    31201 ctcaaaaact cagcattgag cctggctgta atcccctgta cattcttaac atgggcattg
    31261 acaacccctc cctgacggtc aagctcattg accgtttttg atacatcgtt ggcaagctct
    31321 gatataatcg cggctttata atggtaacag ttttccttct tgttcaggtg gttataaata
    31381 gctatggcca tattctcgca cgagttatat ccacaaaatg cacagttgta aaaatccgtt
    31441 tccttcagct tcttcatgtc cacataaatt ctttgcagct cactttcaga cggataacgt
    31501 attttattat tggccgaacg atccacataa gtacgtttgt aaatcttttc atcccaatag
    31561 ctgttaatat tagtttcgag ctctgcggca tctttatact tttcctgagc ttctttattg
    31621 cgttgttcaa cataatactc aatcttgtcc aatggctcat tttggtttag tgtgccgggg
    31681 cctccgttac acccggcatg gcaattgagg caatcgacca aaacaggggc ccttccactc
    31741 tgcacttcgt ctataagcga ttcaaaataa tcataaataa cctcgtgccc ttctatcttg
    31801 cgggacactt tgccaatggt tggcacctca cgttcggcag tacgcaaaag ccctccggga
    31861 gtagaaaaca atacagccct ttcggcaggc gggttgtcaa aatccatatc ctcaaagtcc
    31921 tctaagttaa tatggttaaa atcgataaaa tctttcaaat atttgatggt aacattataa
    31981 tcgcccatgc caacctcgtc aaactcgcgg cgtttggcca cgcatggcga aattaccact
    32041 accttatggt ctttgtattg tggataaaat tcctttatca ttttgatggt atgcatcatg
    32101 gggctgtcag caggggcaag atatggcaac agctccggat gatatacttc caggaacgta
    32161 acaatagccg ggcatggctg tgctattatc atccgggggt tattattgtt caaatgctca
    32221 aggtatgact taaccgtcag ctctgcgcca aagctcacgt caaagttggc ctcaacgccc
    32281 atttttttca acaaagcatt tatcttgaga tattggcccg gaaagttggc tgcaatagca
    32341 ggcgctacaa cagataccat ttttaccccg tgttttaggt catacaaaaa cctttcaata
    32401 tcgtccacat aatacctggc tccatgcgaa caagccttta tacaagtacc acaggccaga
    32461 cacatatcat tgttaacagt aacagtatcg ccacttccat cattacaata cttggcagga
    32521 caaacagata tacacttgtg gcagttgaca catttttcct gtcaaccagc aaaacctggc
    32581 ttagttcgtt tatcatggca tatatttttg gttgctaaat aaaaaatatt aacccttcta
    32641 tcaaatttaa cctttttttt agtctttttc aattaaaaaa acgctttgct tcataaatgc
    32701 tttgattttc gaaatgcaaa gcgttgggct aaaaaaggaa gaatacattt ataagtacca
    32761 agcatatcat attaagtatt tatgcccccc taaccctgcc aagctaaggt ctttaattaa
    32821 ccccaacccc ttaaaaaagg gctttaacaa agcagtgctt ttcagtttta tacaactttg
    32881 ttagccttcc tttttaagga gggatgggag gtttagagat aaaataatac ttagcctgac
    32941 agctatacgc tggcagggta agggttggaa ctataaacaa agtttgatac taacgcgctg
    33001 ttaagcttcc tgtataagag aatccacaat agcagaaaca aactattaca accccttttt
    33061 aaaccaactt tattgttagt caaaaagtat ttatctaatt ttttctcaaa ataagcgtaa
    33121 aaaaagaaac ctaataaaac tatgagcgta aaaaaaacaa aaattcagat acaatgttta
    33181 tgtatgaaaa atttaaaaca atggtgatgt aaaacacctc caaaaaggca acaaatattt
    33241 gttaaagtag tataaaatta ataacttcaa tatagaataa taatcataaa aattatgaaa
    33301 acaataacca aacaaatcac agtattagca gtgtttggat taatttctgc ttcagcttat
    33361 tcgcaggcaa actcatattc ggtcaatagc gaaacgctat accgccatgg gtacaactcg
    33421 gggggagcaa acgtgcagga aaataccgac tctgttacca caggagggac cacccgctat
    33481 tgggtttatc cagacctgtc attgaacccg ggttatgatg taaacaaccc gcttacaaca
    33541 cctttgacct cagcatttaa ctggacaacc tccggggcaa ccggtacagc tacaggtact
    33601 attgcagctg taggcgctta tggtacatat accaactaca aacaagtaac ttggggtggt
    33661 actggtacta ttaatttaaa tgcaacagaa acaagtggtg caggttgcgt tggcggaact
    33721 gctgtaacta ctcctattgc tgttattgca gctccagatg taacagctat tgcgtttagc
    33781 gccctgcctt gccctacagg tactatacct tatacccagg ctggtccgac tgcgacttta
    33841 acaataacct gccctgtaaa tggaaataaa gaagtatcag taaactggtc gctttcatgc
    33901 agcaatgccg ggtggaatgg cggagttgcc atgactggca cagctgtact cggaaatggc
    33961 aacaccattg acttatctgg cgtgaacctt tcgcaccccg gtacttatac cctgaccatt
    34021 gtaaatataa ccgaccgtat agccacaaag tcaggtttaa cagccatccc cgatggtaca
    34081 agcaacacat ttgtattgtt gccaacgccg gttaccggcc ctatgtacca tgttcctaac
    34141 caataatttt tgaaataaca aacctgacag gtaactgcca ggtttttgca taaaatataa
    34201 taattacaat ttttttttat taacatttaa attttattac catgagaaca aaaaatttat
    34261 ttcgttcgat cgttgctgct actttagcag tcgtttctac cagtgctttc gcacaattaa
    34321 ctacaacagg ttctgctact gttgataaag gtactccata tgcttataca agtccaacaa
    34381 gtggtgctaa tccagaagtt gtagattttg taacagttgg tagtacaatg ccttattctg
    34441 tagttccaga ctctactatt caaactattg tagcatctgg agccccattt caagcatctt
    34501 cttacaactg gcgtttagga acaactccac ttggatcttt aagacaagct gatggatcaa
    34561 cagctcttgc taatattgct accgatccag caccttataa tgcaggatat tatctcgatt
    34621 atgatgttgc tgttatttgg ggtaatacgc ctggtattga ttctataaaa gtaagcgagc
    34681 atagcagaaa taatggaggt cttcaaactt gtgatggagc agacactact ttatatgtat
    34741 atgtaatgcc taagcctact gctcagttta ttgaagctgg aactcttgca ggagctacta
    34801 atattgttgg tactgctaac gatgctattg ttggaggttg cggaatagca ggctctacac
    34861 ttcattttaa tgtaactttt actggtactc aagattttta tgtttattat agttctgttt
    34921 atactccatt tggaggatta gctgccccag ctgttacagg aagtgttaca acgggattag
    34981 gcgctgctaa tttttataca gctacaccaa ctgttccaaa tactgacgca gttactgatg
    35041 catttacttt tgcaattcct gctgcaactt atggtaaata tgtatttacc cttactggcg
    35101 taaatgactt agtatcaaga aaatctctta ctgcagcaga tatgggtact ttagttaata
    35161 ctacaggatc tgctccagct ggaccaactt tgacagttta ttcattgcct actcctacta
    35221 caggtagggt attacatgta actaaccaag gttggtagta gatattttaa tttaaccaaa
    35281 atatctcatg gtaaagatat agcccaaaac ggggtattgc ctgagcaggc aataccccat
    35341 agcgggcaat catgagtata aatggttggg aattgttgtg tttcctggta aagggaagcc
    35401 atacatgtgc atataggttt atactgcatt cattatatat gaattgggtt ttgtgcaagc
    35461 atatataaaa gcaatgggtt acttatacca tattatatta tataaaagtg ttaaaaacta
    35521 agcataccat attaatatta ttgctattat ctgtctttgc ctggggcaag gcgcaggtaa
    35581 tggatacggt atgtacacaa agcatcgttg gattctatac aaaaccagat gctcttatta
    35641 ccgactacca tattatgtgg cacttagatt ttgatacttc caaaattgtt gactcaaaaa
    35701 ccacacttaa cggcgacacc ctgaaaatac actttggcaa ctcaaaaggc tggttcaggg
    35761 ttgggggcca gattatctct gacattggtc ctacaggact gaataaatgc gagggcgata
    35821 ttgtttggga ctcggtatat gtatggggct atggcaatat tgaccttccc gacaccctta
    35881 tccgatgcga aaatgaaccg tttgtacttg ctgttgacga aggacttcgg agttataagt
    35941 ggagctcgct aaatggcaat ggaaacgtat atagaggtat agctacaaaa tcggatacca
    36001 tctgggtatc agccagggat cagcacaact gcttattgac cgatactacc aaattgacag
    36061 tttatccgtt gccgacattt gatatcctcg actacagaag tggcaaagct ataaaagaca
    36121 ccatgctttg cggtaccgac caggtaacat tggatgccaa cagtttcgct acatacctca
    36181 actggaacac aggtgaaatc tcgcagcaaa ttgttgttaa ccccattgag ctaaatacta
    36241 aggatactgt caggtattat aaagccaccg ctataatgga ctatgaatat gcaagctgcc
    36301 aatatgaaga ttctgtagca gtgcttgcct gcttcactgg cgaaatcgaa ggtatcccta
    36361 cagttattac ccctaacgga gatggccaca atgacgactg gaatattcct aacctcaaca
    36421 gagaccctgc atgtaccata gacatttacg accgttgggg cagattagta ttccactcaa
    36481 gaggctatgc caaaaattgg gacggcaagg gcaatgacgg taaagcttta cctatggata
    36541 actacttcta tattatcaaa ctgagtggca ataccaaaac tttaatcggt aatattacca
    36601 tcatcaggta aataacgttc atcactaaaa aaatgccatt catgtaaaag actgaagatg
    36661 tttttgaaac aaaaaaataa aaaatttata tttacacgct aaagaaaaaa agttgcatgt
    36721 acaaattaaa aatgaggata ttaactcttt tcctgttaat gttagctata aaaacagtaa
    36781 gtgtttatgg ccaacaagaa ccctcatata cccaatacat gtacaacatg tttttgacta
    36841 atcctgccgc agctggcagt gatggatcta cctctgtaag ttttaccgga agggaacaat
    36901 ggataggctt ccccggtgca ccaaaaaccc actcactgtc agcacaaaca agggtgctga
    36961 aaaatagctt tatagctaaa gccttaaact tacgtaaaaa attcagccgt cgttcgagca
    37021 gcggacgtat tggtgccggt gctcatattt ttagcgacat caacgggcct gtaatgcgta
    37081 ccgggcttca ccttacttat gcttatcaca ttactatcag gcaaagccag ttgtctttcg
    37141 gtttgacaat gatgccttac caatttaaca ccattaatag cttttttaaa aattacgaca
    37201 ctaacgatcc attaatgatc aatttaaagc caaggtatgt tattgatggc aactttggta
    37261 tgctttatac cacgcccgaa tattttgggg ggctatctgt tactaacctt tttgaaagta
    37321 acatcaagtt tggcgataac tcaactgccg gataccaaat attacgtcat tactgtatca
    37381 tgggagggta caaattccct gtcaacaggt atatcaccat agaaccaact tctttaatca
    37441 aatttaccga aatggggagc ttccagatgg acattaccgg tcgtgtattt tacagagatg
    37501 actactgggg cggacttggt tatcgtacag gggctaatgc cggggctatc tcgctattag
    37561 ccggcatacg cttaaaaaaa tactattttg ggtatgcctt tgacttaagt ttatctccta
    37621 tccaacaata ttcatacggt tcgcacgaat tcatatttat gcttaagttt ggcgaaaacg
    37681 cccgtcgtta ccgctggttg aaccgttatt agggtttagg ggttagtgtt tagtacctca
    37741 ccctttcaaa ttcattttaa cttattgctt taaaattttc atgcccttat aatgtaatga
    37801 attttcatgc aatgtctggg gcaaacattg ggctatcgta aaaagtttaa ttttagggct
    37861 gtaacttctt aaagaagaag tagtagaagt ataatcaatc aaagtttcaa caaagaatac
    37921 aaagcgataa taatcaacga taatctttta ccatatcccc ccctttttaa gaggttgttg
    37981 ggattttttt actctcctcc tttctcaaaa gtaacatcgc cgccgctgga taaacggaca
    38041 tcccgtctta tggggctgcc aattactttt acatctccgc cactgctggc ttcggcaata
    38101 atttcttctt ttacacaaac agcaatatca gcacctgatg aagcattagc ttttgtggtg
    38161 ccagctatca aactttttgc tttaatatct gcaccagaag aagctgtagc attaaataca
    38221 gtagcctctc ccaaaatgtt aatatctgcc ccggaagaac tattggcaac aacatacttg
    38281 cccctcactt caacagtaat atcagcagcg ctgctggcat ccatttcaag ataatcagtt
    38341 ttgataaggc ctgcaccttt tatatctgct cctgatgaag catacagctt ttcaatattt
    38401 acagcagtta cataaatttt gactttagta ggctttgagc ggccttcact tctgtagata
    38461 ttcaacaccc cgtcattgtt ttcaaacttt acatattcaa gcatgttttc atcagtttca
    38521 atgaccacac gctcaaaaga gtcctgcact aaagtaacat ccagtcctgc agacacgcaa
    38581 acccctttaa agtccttcac atcaatttcc ttttttacta ttttgccatt gcctgcgatt
    38641 aactccacgt tagagcatga aataaggcca acaaaaaata gcatgatggc cacgagcaca
    38701 gttgattttt ctcttttcat ccgtaaataa tttttttaat aaggccggat gaaccccgaa
    38761 gggctctccg aaggaaaact cgcatgatga attatcatcc ttgtttgtgt ttaacgaatc
    38821 atgctcgttt cattttatta tagtttaatt ttgaaactat gacggtcgga gagaatcaaa
    38881 agttacagaa aaaaactaca aatgtattac ttcgccataa gcctgggcag cagcctccat
    38941 aatagcctcg ctcatggtcg ggtgtggatg tatggcttta atcaactcat gtcctgtagt
    39001 ttcaagtttt cgggcaacaa caagctctgc aatcatctcg gttacattaa ggccaataag
    39061 atgtgcccca agcaactcac catattttgc atcaaaaata agtttgacaa aaccatcttt
    39121 actgcctgct gcactggcct tacctgatgc catatacgga aatttaccta cctttatctc
    39181 gtgccctttt tctttagctt ctttttcggt catgcccaca gaagcaattt cgggagttgt
    39241 ataaacacat gccggaatat tgctataatc aacaggttca ggatttagcc cggctatttt
    39301 ttcaacacaa acaatacctt cagccgatgc tacatgcgcc aatgccggcc ccggaacgat
    39361 atcgccaatg gcataaatgc cttttacatt ggtttggtaa tattcatcaa caacaacttt
    39421 acctttctca actttaatgc ccaaattttc aaggccaata ttctcaatat taggagtaat
    39481 gccaacagca gagagtacaa cctcagcttc cttaactatt tcaccattag gtgtttttat
    39541 cttcacttca caaacatccc cttttacttg aacagcctca actgaagaat tggtaagcac
    39601 ttccatgcca tcctttttaa acgaacgttc gagttgtttt gacacgtcct catcttcaag
    39661 cggtacaatg gcaggcagaa attctaccaa cgtgactttt gttcccatgg tattgtaaaa
    39721 cgctgcaaat tcgctaccaa tagctccaga acctactact accattgact ttggaagttt
    39781 tggcagcgtc aaggcctgtc tgtagccgat aattttcacc ccgtcttgtt tcaaattggg
    39841 caactccttt gaccttgccc cggtagccaa aatgatatgc tgtgctgtca attctttctt
    39901 tgagccatct gccgcttcaa cctcaacagt attaacagct tttagctttc caaaccctgc
    39961 aataacctca atcttgtttt ttttcatcaa aaactgaacc cctttgctca tgccttcagc
    40021 aacagtcctg ctgcgcttta caacagcgtc aaaatcaaca gcagcctctc ccggcagctt
    40081 aataccatac tcagcagcat gtttggcata actataaacc tgggcactct tgagtagcgc
    40141 ctttgtagga atgcaacccc aattaaggca aataccccct atttcactgc gctcgacaat
    40201 agctactttc tttcccagct gtgaagccct gatagcagcc acataacctc cagggccact
    40261 tcctatgaca atgatatcgt agtccataat attagttttt ctgttttaat ggaattaaac
    40321 aacttaaatg aaaaaaaagt tcagttttaa tactaaccct aaaatcccct aaaggggact
    40381 tgttacaatc aagcagttaa aatcattttt agaaatttca agtcttcctt cattaatcat
    40441 taaccattta ccattaataa ttatcattta tcattcaaca ttaacactca ccttataccc
    40501 gccaaatttc ctgatgttca acactccccc atcttcaaat atcaggtatt gccctcttat
    40561 cccggacaat ttaccctcaa tatcaggttg tttttccaaa tccaggcttt tgaccttctg
    40621 aggataagca tttacaggat attgaaaatc tactacctgt tcattttctg aaagataccc
    40681 aagcaaatca gaaggtaata cggatgctac tttgtcaaaa atagcatcaa atgaaggctc
    40741 tttctctact ttgttggtaa gcatagaccg ccagttggtt ttatcaggca gatactgttt
    40801 cagggcaact tctatgcagc ctgccagata cctgtttggt gtacgagcta taatacgggt
    40861 tttttcagca ccctggtcaa tccatcgata aggaatctgg gtatgtcgcg ttatacccac
    40921 ttttatatgg tcaacagaag ccaggtacac aatatgttca atgaggcaat gcgctttggc
    40981 aaactccata tctctggcta ttccttcgtg ggcacgacaa agctcgggac gaaaaacaca
    41041 ttcttcggtt tcgggggcag aggtaaaaca tgtataacaa tagccttgct gaaatgaggt
    41101 tttggtgcgc tttccacatt tgatgcagta tatctcgcca gcataactaa tgcgtacaag
    41161 ctttccaagc cattggttga tgtttatttt ctcatttcct gactccagaa aataggttat
    41221 tggcgatgta tgctcggaaa tcagttttcc taaaatgcaa ttgtaattca tgagaaaata
    41281 tttattataa actttttggt actatggaat atccatgata cgatttttat attcttttta
    41341 gtgagttcag ttacatcatg gatgtttcat tatcatccta tataaatcat ccgtatttac
    41401 gaactttatc catttcttct tttgcaagcc tcttatcagc cttttatccc cgctccataa
    41461 ttttcccctg atatgttcag ttaatgcaac aaattcgcaa tcgtcaacat caacatcttt
    41521 cgttaacaat tcacaatcat aatatatttc tttggggatc aggctaatat taacaaatct
    41581 gatgcgactt gttatcaaac ttatcatcct gtctaagtca taattggaat accctgtcaa
    41641 gctaatgatt ttatcgcgat ggctactaat ctcgtatata agtcgctcag tagaataaaa
    41701 atttaacctg gtcttaggtt gcagaattat ctgggaaata tatgaattgg tgctaagtaa
    41761 ggcgctaaat actatgttgg tatctacaac aatcctcata gattaagcgt cgatttagtt
    41821 gcagcccaac gtcctttctt tattgtttta accagttcat caacatcttt ttgggttgct
    41881 tttgatttct tggcaatttc tttatattca agcaagtcgg ccatatcctg caactcatct
    41941 acattggtat ctgctggcaa cctgaaaatt atttccttat ttgtcctttc tataatcatc
    42001 gccttacaat ttaggtaaag ttagtttttt tgagtcattt ttgcaagaag ggagcacttc
    42061 ctcaaatcca acgcctgact ttacttttat aagcatcata ttcctggcca aaagcctctt
    42121 tcataagttt ctcttcctga ggaataacaa gcccattgac gagcaacaca aaaacaaaac
    42181 aagcaaggat tgacaacata ttggtagaac aaatgccaaa ccccaacaac aacagaaaca
    42241 taccaatata catcgggttt cgcgacttgg caaaaacgcc ttcggtaatc atggacgaag
    42301 atggctttat ttctagtgta gtattgtgct ttttgaacaa gtcccaggtt ttgctcatga
    42361 tggcaaagcc caatacaaca cttaacaacc cagccaggtt ataagggaaa tctatcacgt
    42421 tgaactttgg acagcaaaaa tagaatgcta cagtcagaat caaagtcaac aaaacaaaca
    42481 caggaggaac aagcagcttt ttcattattc attgtccatt attcattctt cattattcat
    42541 tcttcattat tcattgtcca aatgcttgcg caaaatcggc aataatatcc tctgtttcct
    42601 caatacctac tgatagtcgg attaatcctt cattaatgcc catagccctt tgctgttcag
    42661 gggtaaaatc acaataaata gtagaagccg gatggattat cagggtttta ttatcgcaca
    42721 tattagtagc acgttttacc aatttcagcc tgttgataaa cttcatacat ttctcagcgc
    42781 tctcaagctc aaatgacata agtcccccag gaagggctgt aaactgtttt cggcaaacag
    42841 cctcatactt tgatcctttc aaagcaggat aattaaccgt tttcacctct gcattattgc
    42901 tcaaccaggt ggccacagcc aaagcattgg cagccatacg ctcaaaccgt aactgcatag
    42961 tttcaattcc caaactttgc aaatatgcat tgaacggaga aagacaacct cctgtattac
    43021 gcagtatatc tttcctcaaa cgggctaaaa aagtcaattg cccagatgta gggacaaaag
    43081 gcttcaactt tgggttattc tcccaattat atgtgccatg gtctataacc aaacctccaa
    43141 ttacagcagc tccgcttgaa atatacttag ttgacgaaac caggctgata tcaataccga
    43201 acttacgggc attgaacata ttgggaggcg taagggtagt atctcccaaa actacaaggt
    43261 tatgtttatg agcaatagca gatattgacc caacatctgc tatttcaaca aaaggattat
    43321 taagcacttc gaacagcaca gcacgtgtat tgccatcaat aagaggttct attgtttcgg
    43381 gttttgtgaa atctgcatat ttaaaggata ttccaaaatt attcagggtg gactcataca
    43441 aagcaacagt attgccaaaa agcttgttgg tagtaacaat attgtcacca gctttgcaaa
    43501 cgttcattat tgcagcagaa acagcagcca tacctgaaga aaaagctaca accccaatag
    43561 cttcggtaat gctttttacc tgcaactcaa aatgctcaac agttgggttt gctgtacgtg
    43621 aataagcagg ggcaatcatt gtgccattaa aagcaccggc aatactttcg gccgattcaa
    43681 actcaaatgc cgaacagcca tatataggtg tttgaagcgc cccataggca tcaggcctgg
    43741 ataatgctgc atgtaacaaa cgagagtgaa accctttcaa atctttattt cccattgttt
    43801 ttcttttatc tttattcttc tttaacttcc tcgattttcc gtattcttcc taaatataaa
    43861 acatctcatt atcccccaac atttccatct tttctaccaa cgatgcaaga gtaatatttt
    43921 caaaaaaacg ccccaccgat tcgtccatct cgtcaaacat tgcacgcaaa accaggtctg
    43981 tatgcaaagc cggctcattg tatggtggct gaaacccttt actgtcagac tctacggcta
    44041 caataatgct tttaatagta atttttgaag gtgaattgcc aattaaatat ccacctcccg
    44101 caccacgctt tgacttcaca atgccatttg atttgagcat agcaacgatg ctttccaaaa
    44161 acttttccga aatattctcc ttatgagcca accctgctac tgtcagcctt ccattggcag
    44221 gcaacgttgc aaggttcagc atgaacttca aagcataaaa cgatttattg gtaattttca
    44281 tccgtatgca agattaaaac acaaatttta taccttcttg gcagcctgca tcatattgag
    44341 tgcagtcata attcgcgaaa cggtagtttg cggattaata tcgtcggtga gaataacaat
    44401 tctggacttg ccataaaaag gttcccttat ctttaatgtt ttttcaatat attcagctaa
    44461 ctctgattct gttttgcctg ccaataaagg ccttgacaat cttgccttct ccgaaaggct
    44521 cattaaccta tgagccaaag cttttgctga cattttaaga taaatagtaa tgccgtattg
    44581 gttcatccat tccatattgt cgaaaaaaca tggcgcccca ccaccagtgg caataagcag
    44641 cttctccttc ttacctaata tgtgcagcgt gtctttttca atttcccgaa aagcattttc
    44701 tccctttgta gcaaaaatct ggctaatagc catctgctgc tgctcttcaa taacagcatc
    44761 catatcaacc agttgcaggc cgagctcctg ggcaagcatt ttcccaatag tagatttgcc
    44821 gcaacccata aaaccaacca gaaaaatatt cgtcatattc taactttttg agtcaaaagt
    44881 acttgtttta gcccaattag ccccagaaaa tacttaattt tgttcgatac actctttcct
    44941 tatttcaatt ggagaacctc gattacatac attcgttaat aaacctctgt tttgcagaaa
    45001 acaaagagtc ctttgtccat tcatacaatg aactgcaaca aaaaagcaac tggaaaaaca
    45061 ttgcaaaagt caactttctg cttccgcttg tcttttatcg tgccaaaaaa ctgggagcat
    45121 tatctgcatt gcaacaaaaa gactcagaac tggcagataa acagttgaaa agggcttcag
    45181 tgaaaaattt aaagctaaac catgcttttt caaagcttta tactattgcc ctcaacaaca
    45241 atattgaaat tacagcaatc aaaggcgtgt acctgcagca ctacttgtac ccagagtctt
    45301 atctgagaca aatggtagat atcgacatcc ttgttgccgc aagcaaagta gaagaactcc
    45361 gcaaaaaact ggtcatcgaa ggcgctattc ccattactgt gcatcaaagc cgctttatag
    45421 aaagcctttt gcaccaaatt tcgccaatta agctaaacga tgtcagtatt gaaatacatc
    45481 gcaacctctt tgattcgttt gatgaatttg tttgtcctga agccctgata gcaagcaaca
    45541 gcagggtcga aaccctttca ggccacaagg taaaagtgct gaacaatgag tttttgctgg
    45601 tttatttgtg ttgccacgta tttaacacca tcaggggcgg aagtctcagg ttaatatcac
    45661 taatagatat ttttctcctg ttgaataaaa gtaacatcaa tgttgagcaa ttaaacagca
    45721 ttgccaaaga aatgaacgct acgttagcgt tacaaaatat tttgtactta gtaagtaagt
    45781 tgtttaaggt ggagacccca tgttgtccct caactcctga aaatattgaa ctttttccta
    45841 tcagcctgga ttatgccata aagattttct tgtccgaaca acctccattt gacacaagcc
    45901 actattttac taaattcaaa aaaataaacg gatttaaaaa taaaacgaaa tatatattac
    45961 acgctgtttt ccctcaacaa gaatatataa aaaataggta tttac
//
LOCUS       DAOSPF010000025        45541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_861_length_45541_cov_7.795080, whole genome shotgun sequence.
ACCESSION   DAOSPF010000025 DAOSPF010000000
VERSION     DAOSPF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 45541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_45541_cov_7.795080"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..2366)
                     /locus_tag="PK252_06795"
     CDS             complement(21..2366)
                     /locus_tag="PK252_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT14453.1"
                     /translation="MAETVDKTNKYKKILWVLFVTPFFLVALIFILISIGAFGFMPTF
                     EDLENPNNNIASEIIAEDGVMLGTYYYQNRSFVDYDKISPNVINALIATEDVRFYKHS
                     GIDIRGLARVFFKTIILQSNSGGGSTITQQLAKNLFPRDTSLYNNFLSKSASLAIAKF
                     KEWQTAVKLERNYTKEEIITMYLNTVPFGGQAYGIKMAAKTYFNKSTDSLNIEEAAML
                     VGMLKAPTRYSPVQNPERALRRRNVVLSQLEKYDFINKRQCDSLKNLPLIVDYKAQDH
                     TEGLATYFREYLRTTLTANKPQKKNYYDIEDYKRDSVEWCNNPLYGWCNKHVKPDGTT
                     YNIYSDGLKIYTTINSVMQQYAEDAVYEHVGKYLQPAFFKEKKGRHDAPFASNLTADQ
                     RQQIMDQAVKRTERYRVLRNEGWSFEDIMKDFKKKHKMRVFTWNGERDTILSPLDSIR
                     YYKFFLQTGLMSMDPHTGYVKAYVGGIDYKYFKYDHVTISKRQAGSTIKPFIYTLAMQ
                     EGYSPCDKVPNVPVTFILGDTIWSPKNSGRSDYEGKMVTLKWGLANSVNFISAWVMKQ
                     FNPPSVIDIMRKMGFTSYIAPYPSLVLGTSDVTLYEMTGAYGCYANKGIYVKPMFVTR
                     IEDKTGNVLATFTQEKTEAISEQTAYLMVNLMEGVVNAGTACRLRYGYKFTGPIGGKT
                     GTTQNHSDGWFMGFTPDLVTGVWVGGEDRAVHFDNIDMGQGANMALPVWAIYMDKVFK
                     NKSLRCNSKSFERPYNFNVDIYCTDTDEKSLNEEDIRMMNE"
     gene            2495..4822
                     /gene="topA"
                     /locus_tag="PK252_06800"
     CDS             2495..4822
                     /gene="topA"
                     /locus_tag="PK252_06800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT14454.1"
                     /translation="MVENLVIVESPAKAKTIEKFLGKSFTVMSSFGHIRDLAKKNLGI
                     DIKNNFNPDYVVSDDKKKIVAELKKNVKEAKMVWLASDEDREGEAIAWHLAEVLQLDE
                     KKTKRIVFNEITKEAIQNAIKNPRNIDKNLVDAQQARRILDRLVGFELSPVLWKKVKP
                     SLSAGRVQSVAVRLLVDREREIIAFKPSSSYKTVAVFEVVDNKGKKVLLEAELDEKFN
                     TKAKALEFLKACSKAQFSIEDVVTKPSKRSPSAPFTTSTLQQEASRKLGFSVSQTMTI
                     AQKLYEAGKITYMRTDSVNLSETALKACKEVINKEFGNEYHNLRQYKTKSKGAQEAHE
                     AIRPAYFDQETVQGSAAEKKLYSLIYKRTLASQMSDALFERTTVSIKVEGLPYHFVAT
                     GEVLKFDGFLKMYIESNDEESESDNLKILPPLTKNQPLNVKNITSTERFTHHPPRYTE
                     ASLVRKLEELGIGRPSTYAPIISTIQQREYVVKTDRDGSERNFDVLKLENKNVTELTE
                     KETFNAEKAKLFPTDIGILVTDYLVDHFKDIMDYNFTANVEKDFDEIAEGKMLWTKML
                     DAFYAPFHKKVEMAIATRENTKGDRLIGNDLVSGKPIIARIGRFGPMVQIGQSDDEEK
                     PRYAKLQPGQYIETITLEQALNLFQLPKKLGSYENYEVSVGIGRFGPYILHHAKYYSL
                     VKGVDDPYNITLQRAIELINDKREKDKNKVINSFEYQKSEIQVLNGRFGPYICYNKEN
                     YKIPKNTDATKLTAEQCIDIIQKGASTTKTKKKRK"
     gene            4825..5979
                     /locus_tag="PK252_06805"
     CDS             4825..5979
                     /locus_tag="PK252_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOT14455.1"
                     /translation="MLLSDYLLPVSLDKPNDEVLQNKNSFCRHIDINTANKPVDLDKK
                     KYKLAIIGVPEYRGCGNEGVVNTPNAIRSELYQLHYFEKKIQLADLGDLKIGKELTDT
                     FYALREIVLELHARNIVPIVIGGSQDLTYGMFLAFEYLKVPYNVATVDFKLDLAFDSY
                     TPINYQNYLNTIILENKYLFEFFNLGHQACFSNYDGNFLLDTLSHEKVRLGNMRNQTE
                     KLEPYLRDTHLLSIDFSAIKHADAPAQLVSSPNGLYAEDFCQCMHYAALSENIKAVGL
                     FNIDASKDYQGLTSKLAAQGIWYFIEAFSRRNIENPSKSSSKFKKFSVTLNEDTSIVF
                     LKSNRTGRWWFEISDKKNQKIYFACAENDYLTAINNDIPERWLKLFKKLN"
     gene            6165..7175
                     /locus_tag="PK252_06810"
     CDS             6165..7175
                     /locus_tag="PK252_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOT14456.1"
                     /translation="MDNNIFKSNLCFLVRIFYASKGIAVRKGLFYTVLFLLLGPSFFT
                     RAQESQFTHFMYNNMGINPGYAGSTEMVGINIIHQNKLMGFEKAPRHDIASIDIPFTL
                     FEKSHGVGLNLINSSMGVNNDIGMQLAYAFQRKMAIGDGKLGIGICGGFNRSTFKAGD
                     LASGTDPLVPTTEATPMVFDFGTGVFYNSEKIHMGISVAHLITGERDYKIAAGDVMPL
                     QRTFYVTAGYNYQLPNPMLMLCPSFLVQTSGNLTAVSFNTNIVYNNRIWGGFSYSAQN
                     SVSAMFGLELLPGVKFGASYDYSVNYLRNGTGGSFEIFANYSFKLKKEKIPQRYKSIR
                     YL"
     gene            7248..8585
                     /locus_tag="PK252_06815"
     CDS             7248..8585
                     /locus_tag="PK252_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOT14457.1"
                     /translation="MRKFVYLSVVILVLAGCGKPSDTGELTGVSDGRKYFEPDPYGMV
                     FIPQGSFNMGPSDQDVAWAQNSLSKTVSIAAFWMDDSEITNDEYRQFVKWVQDSIMRR
                     MLAAQIEDFAIAEDELGNPIDPPFLNWEMEIDPQDEEQSGIINELYLTEQERFYRRKE
                     IDTRKLNYEYYWVDLKQAAQKGNRYNFATQAYEGTVINSKGESAQIINRSSFVLQDFI
                     NVYPDTLCWVADFTYSYNEPMANMYFWHPAYDNYPVVGVSWKQATAFCIWRTQLLNTT
                     LQKNGELFVHDYRLPSEAEWEYAARGGLDLSMYPWGGMYTRNNEGCFLANFKPLRGNY
                     TDDGGITTTRVGTYDPNEYGLYDMAGNVAEWTSSAFDESAYIYTHDLNPDYKYNALPD
                     DAPVLKRKVIRGGSWKDIGYFLQVGTRSYEYQDTAKSYIGFRCVRTYLGNSNQ"
     gene            8644..9564
                     /gene="gldL"
                     /locus_tag="PK252_06820"
     CDS             8644..9564
                     /gene="gldL"
                     /locus_tag="PK252_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOT14458.1"
                     /translation="MSKLYGYGASVVIIGAMFKIMHWPGAGFMIVAGLSTEAIIFFFS
                     AFEPLHEEVDWTLVYPELAGITDDDEMENYKESVTNSRGNVVLEKFENLFEQSSLSPE
                     TLEKLGEGFAKLSDTTSRLSDISEATAATQAFASNFRNAAQSLDSMTSLYSENSEALS
                     SSISSLSKTYQKTAEIIEKTGVQVSAQVEKSGLDMANSYQKLSESIASYSASVNNSGQ
                     NYQNQLEKINKNLGELNTLYELQLKETNDYVKHNEQLYAGLSNIIGNLSESVAETQRY
                     RQEMAKLSENLSSLNNIYGNMLSAMNYSKK"
     gene            9576..11189
                     /gene="gldM"
                     /locus_tag="PK252_06825"
     CDS             9576..11189
                     /gene="gldM"
                     /locus_tag="PK252_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOT14459.1"
                     /translation="MGHGKETPRQKMIGMMYLVLTALLALNVQKEVLNAFVIVDEGMT
                     KMNENYSKKNEEMYFAFDQANAQNPAKSKKWRDIAYTVKQKSEELYENIQDLKLKILS
                     TAGEVEAVKGREINTMLINGKENTDVPAQVMIVEGNGKKLKAMIEDYRRYLIEQIDPK
                     AEALINSLETGLSTKDPEPVGGKTESWESEHFEHLPLAGVLTIMTGLQSNVKNAEADV
                     LRYLYTMIDKGSFKFTSLEATVIANSNYVIQGNEYQAKVFMAAFDTTQDPEIYLGPCD
                     SVRTEEGYVYTEKKGFNYGAPLPVKKGKGIYSVRATAPGEKNLVGIIKLKAPSGGQDI
                     IRPFKTSYRVAEPMLVVSPTKMNVFYLGVDNPVEISVPGVEAEKIFPSISNGTITKSG
                     KSFIVRPSKSGIAQINILAEIDKQKKQMGAKEFRVKIVPDPVAKVAGIKGSGVIDKNV
                     LLAQSGIVAEMENFDFDLQFKVTEFKVSVNVGGFSNDKTSKSNKFTPEQFNLIKGANR
                     GQKVYIEDVKAVGPDGSIRLLGSIALTLK"
     gene            11217..12083
                     /gene="gldN"
                     /locus_tag="PK252_06830"
     CDS             11217..12083
                     /gene="gldN"
                     /locus_tag="PK252_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOT14460.1"
                     /translation="MKRALLFIAGLSILCSAQVVQAQDNKTEVYVKEHIPNKKPVPYP
                     YMREADVMWAKIIWRVIDLREKMNMPLYYPTKPIDKRMNLITLILKGLDDDSLGIHAY
                     STDDPLNEFKDPPLTKEAINEQMGAKVDTLKVTDVNTGDLETKIITRERQIDAVKQIM
                     IKEKWFFDKQHSTMQVRILGICPIIVRNKEVNGQITDEIEKKLTFWIYYPEIRNLFAS
                     YEIYNRFNDAQHISFDDLFLQRRFSSYIFRESNVYNNRSIGEYTSGIETLYESERIKE
                     SLFNYEHDLWEY"
     gene            12146..12631
                     /gene="rfaE2"
                     /locus_tag="PK252_06835"
     CDS             12146..12631
                     /gene="rfaE2"
                     /locus_tag="PK252_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOT14461.1"
                     /translation="MLSIEKKILTVGALLPELARLRFQDKKIVFTNGCFDILHRGHVD
                     YLSRAAELGDVFIVGLNSDASVSRLKGSTRPIQDQYSRSIILAALQCVSYVILFDEDT
                     PYNLIKAVQPDILVKGGDYTEISKIVGFDIVTQRGGKVMTIPFVEGFSTTGILEKISK
                     L"
     gene            12749..13966
                     /locus_tag="PK252_06840"
     CDS             12749..13966
                     /locus_tag="PK252_06840"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT14462.1"
                     /translation="MEKSVAILCAGGPAPGINTVIGSVTKVFLKNGYKVIGIHEGYKT
                     LFKGEPNVEFLDFQFADNVYSRGGSALRMSRYKPKDEEFKPDFFERFNVKLLVTIGGD
                     DTASTAARLTKYLQSKNLDVKNIHVPKTIDNDLPLPEGIPTFGYHSAKEEGVRLATTI
                     YEDGRTSGTWFVVAAMGREAGHLAFGIGTAVHYPMIIIPEMFNKTHVTFEKITNLIIS
                     SILKRNIMGIDYGCAIVSEGVFHFMSDEEIIASGIQFTYDDHGHPELGNVSKSHIFNY
                     LLQNKLKALGLKVKSRPNEMGYELRCCRPIAFDLAYATQLGLGVYKLFSEGKSGCMVT
                     VDHVNNITPLYLKDVSDEKGKVIPRLVNIDSSRVQMVFESNLHYIAPEDYEAAKKFVK
                     NPEEFDFKKILNW"
     gene            14116..14775
                     /locus_tag="PK252_06845"
     CDS             14116..14775
                     /locus_tag="PK252_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT14463.1"
                     /translation="MILLDATTVAVTKSESMIDIMLKGGWILVPIFLMSIFAVYYMVE
                     RYTVFQHALKVDKNIVDDICGHIKHGNLAKAHELASGYNHPIGRVIVAGLSNPARKIE
                     DIEQIMAEETKNEIFILEKNLGYLGSIATIAPMFGFLGTIFGVIKIFYNISLADNISI
                     GLIAGGLYQKMVSSAAGLTVGIFTYAGYYYLNNRVDVFVNRMERNLSPVVNAIRDSRL
                     S"
     gene            14788..15186
                     /locus_tag="PK252_06850"
     CDS             14788..15186
                     /locus_tag="PK252_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT14464.1"
                     /translation="MKIERRKSRTSEVYTASLNDIMFFLLLFFLIISTMVNPSVIKLL
                     LPKASNSEQTVSKQTINLAVTKDLKYYLNDEEVPFDKLEIKLKILMNRAKETTVVLRA
                     DNTITLQPVVDIIDLGNKLKIKVILATRKD"
     gene            15270..15956
                     /locus_tag="PK252_06855"
     CDS             15270..15956
                     /locus_tag="PK252_06855"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOT14465.1"
                     /translation="MKAIKLLIIAGLLLSVNLYSQKIKSFKVEAFNQLEVYGKFDIEL
                     KKSSEFSVKLYSDNIDLSKVDVVVENKKMKISALSGLFKKNSKVSVTIEYDSLNSLLV
                     TNGAEIYSSKPVQVNNLKLVAGNGSTVNLKFETESFTAYADKGATINAEGKSKFLDIE
                     VASGGIMNGFEFICDSADVRCNAGGMVKINAKRYLKAAASTGGEIIYRGKPVLNQRTT
                     LGGKIEQMKE"
     gene            complement(16177..16848)
                     /locus_tag="PK252_06860"
     CDS             complement(16177..16848)
                     /locus_tag="PK252_06860"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT14466.1"
                     /translation="MKLFVIIVAGGTGQRFGSAIPKQFLLLHGKPILMRTIEAFLAYD
                     PSMQIIITLPPDYIQLWQSLCKQYNFTVPHIFVEGGQTRFHSVKNGLNQIKEAGLVAV
                     HDAVRPLVSTAVIERCFQTAKIKGNAVPVIPVVDSIRQMTENGSVPVNRENFMQVQTP
                     QVFDVLLLKNAYNTEYVPEFTDDASVVEKLGKEIFLVEGNKENIKITSQTDLSIAEAL
                     GHYFL"
     gene            17492..20716
                     /gene="carB"
                     /locus_tag="PK252_06865"
     CDS             17492..20716
                     /gene="carB"
                     /locus_tag="PK252_06865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOT14467.1"
                     /translation="MDKTIKKVVLLGSGALKIGEAGEFDYSGSQALKALKEEGVYTVL
                     INPNIATVQTSEGIADKIYFLPVTPYFVEKVIAKEKPDGILLSFGGQTALNCGTELYK
                     TGVLEKYNVRVLGTPVTAIMNTEDRELFVNILNEIDVKTCRSEAVTTVDDAIKAAERL
                     GFPVIIRAAYTLGGQGSGFASNVEELRRMAEKAFSYAPQILVEESLKGWKEVEYEVVR
                     DKYDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNSEYHKLREIGIRTIRHIGIVGE
                     CNIQYALDPYSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLALGYGLHELKNSV
                     TKTTSAFFEPALDYIVCKIPRWDLNKFIGVSKQIGSSMKSVGEVMAIGRSFEEVIQKG
                     LRMIGQGMHGFVGNREIEIADIDKELTEPTDMRIFVIEKAFENGYSVDKIHDLTKIDK
                     WFLYKLKNIFDHKEALEKFDSIEKVPDIMVLEAKKLGFSDFQIARSVSKDENAKMEQA
                     MLDVRKNRIKRNILPYVKQIDTLAAEYPAQTNYLYLTYHANEHDIPFFSDKKSVVVLG
                     SGAYRIGSSVEFDWCSVNAVLTAHKLNYRSVMINYNPETVSTDYDMCDRLFFDELSFE
                     RVMDIIELENPKGVILSMGGQIPNNLAMRLYHQNVPVLGTSPISIDRAENRHKFSSML
                     DKLGIDQPRWKELTSMDEIDDFIDKVGFPVLIRPSYVLSGAAMNVCSNREELEHFLKL
                     AAKVSKQYPVVVSEFIENAKEIEIDAVAKDGEIMAYAISEHIEFAGVHSGDATLVFPA
                     QKIYFETSRRIKKISREIAKELNISGPFNMQFLAKDNDIKVIECNLRASRSFPFVSKV
                     LKANFIELATKIMLGAEVSKLDKSYFELDYIGIKASQFSFSRLAKADPVLGVDMASTG
                     EVGCIGEDYYEALLKAMLSVGYRMPKKNIMLSTGEMRSKVELLSGVKMLAERGYNIFA
                     TKGTAAFLEQNGIKVTTVAWPDEDAKDNVMEYIRGKKFDMVINIPKNLSKTELNNDYE
                     IRRGAIDFNIPLITNARLASAFIHAICKKSIDDIKITSWDEYKS"
     gene            20892..23306
                     /locus_tag="PK252_06870"
     CDS             20892..23306
                     /locus_tag="PK252_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14468.1"
                     /translation="MRKTFALIAILTLAGQTFAVENKAAMGTISGKIVDGNDNTPLEF
                     TNVAIYRQSDSSFVQAASSSADGIFKAENIPGGVYKAKVIFVGYKTSVIDSIRVTPFK
                     NTFVGEIKLFANTKNIGEVVVKGERSIIESKIDRKVFYVDKNIAAQSGNATDVLQQVP
                     SVQVDAEGTVSLRGSENVNIMVNGRPVMMDKTTFLQQMPANTIEKIEVITNPSAKFDP
                     EGTGGIINIVLKQGAGTGTSVVISANASTNDKYNFSANVGVNPGKYNIYASYGLRDDT
                     RKINGRQFTTYLHDNSTHQSISEEKRTNLSHMGKLGMDYNFTRTLTAGVSGSYSSGDR
                     NGDERNNYTETDTIVTLWYRSSDEEETNTRIEANSYITQKFDNNGHEAKLDYTFNKEN
                     ETERNNYKDRYSLSEAFISDELQDNTSNYRRHTIQADYTLPLDNGLKIELGTKEIFYM
                     SETDQKTFYDSTGTNALIADPSRTSLFHYNEDIYALYGTLSYNYGNFGVIGGFRTEYF
                     KSTFHFDENDTYSNPYWSYYPSIHTTYKITEKHEVSLSYSKRVNRPRGFMINPFPDYS
                     DPLHLRKGNPNLKSEYINSLEGGYTFKIEKITVQPTVFYRFTDNAFTNLAIVDTLTNV
                     TTSTFENSMKRSSWGTDLSVTLQPFKFWNINISASAYQQELNASNLNSSTKSDFGYSG
                     KIMSNTFLPLGFAFQVNAFYRSPFITPQGKSDPFYSMNLGLRKDFFKGKLTGNIMFND
                     VFDNTHFGMNIKNKQQEGEVYRKFDSRTIFVGLTYKFGKQSVNKKKQNNNQNNDEQNG
                     NEEMMY"
     gene            complement(23483..24088)
                     /locus_tag="PK252_06875"
     CDS             complement(23483..24088)
                     /locus_tag="PK252_06875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT14469.1"
                     /translation="MLLYLPGFGDMEGIVTVTNVLFPNAKRFFILSKTLPLRQQKSMT
                     NTFTAIDFETAQGYRHSICQVGLVRVVDGQVSETLNMLVQPPGNYYWYRFTGIHGISA
                     KDTMHSPTFDKVWPKIEKFIAGQTVVAHNGFGFDFPVLAYTLAFYGLPVPDYTKYCTY
                     KIYKSNLAALCSRYGISLNHHDALSDAIACAELFLMHVSRK"
     gene            24171..24947
                     /locus_tag="PK252_06880"
     CDS             24171..24947
                     /locus_tag="PK252_06880"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOT14470.1"
                     /translation="MNKLNALLIALMLLSVSFSSAQQYKPIKILPNTFFKGGEKLTLQ
                     LRYGFIIGGNVTLELTESNDLLHVKGTAKTTGLADKLFSVKDVYESYFMNSNNLPVFA
                     IQNVKEGKKYTYYNEVKFNHALKKVTSTKSGDHDVPEGIVDMMSVFYMIRRLDLSEAK
                     EGDIYKINTFFSDKLFPFELRYRGKETVETPLGKIRCIKFAPIVEPGRVFKSKDDMFI
                     WFTDDENRLPVLIAMEMLIGSVNCEMIKYENVMKEPVFKK"
     gene            complement(24958..25701)
                     /gene="kdsB"
                     /locus_tag="PK252_06885"
     CDS             complement(24958..25701)
                     /gene="kdsB"
                     /locus_tag="PK252_06885"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOT14471.1"
                     /translation="MIAVGIIPARYASTRFPGKPLVDIGGKSMIRRVYEQASKALEFV
                     FVATDDQRIFDHVEGFGGKAIMTQTTHKSGTDRCAEAIRKIQAEVSQKISVVINIQGD
                     EPFIKPEQLRLLKSCFEDENVQLATLSKVIDSAEELFNPNVVKVVRDKDNKALYFSRS
                     TIPYMRNFEPEHWVSSHKFLKHIGIYGYKVETLKEITRLAPSSLELAESLEQNRWIEN
                     GYNIYVQTTAFETVAIDTPEDLEKVKGLL"
     gene            25809..26612
                     /locus_tag="PK252_06890"
     CDS             25809..26612
                     /locus_tag="PK252_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT14472.1"
                     /translation="MKIVTLIENLVQQTNYVAEHGLSLYIETGGHRLLFDTGLSGKFI
                     DNAVKMGIDLAQVDAVVISHGHYDHAGGLNAFFEINAKAKVYAQKSAFLPKFSRGFKY
                     IGNETNAEVLAKRLIELNEVTEIVPGVYLFPQTKLFDRTDTNFKDFYFQEGDTKRPDE
                     FDDEQFLVLKKNSEINILTACSHRGITNVCATAVEYFKLPVGMILGGFHIKDCPESQY
                     QFMIEYLKRLAPKYIGVSHCTGVDKFARMMNDCSAKVFYNQTGNEINLK"
     gene            26613..27230
                     /locus_tag="PK252_06895"
     CDS             26613..27230
                     /locus_tag="PK252_06895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT14473.1"
                     /translation="MAENTFDIRAREWDKNRVHFDRSEAIAKRINAVIPDGRKMKALE
                     FGAGTGILSFLLKDKFSEITLMDSSQEMINVCTEKIEEYNTPHIKAIKYDLEHSLYPE
                     TFDIIYSQMVLHHVKDYKKILYTFSSMLNDGGYIALADLYPEDGSFHGEGVEVHKGFD
                     PDELSQMLSEMGFKPILEAKVFELKRDNGIVYPVFLLVSVAGMKR"
     gene            complement(27343..27483)
                     /locus_tag="PK252_06900"
     CDS             complement(27343..27483)
                     /locus_tag="PK252_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14474.1"
                     /translation="MIDEDKLFPDIPEFPDNYVEKGMNESKLTDFKFTPPPPPPPPAD
                     NE"
     gene            complement(27495..27665)
                     /locus_tag="PK252_06905"
     CDS             complement(27495..27665)
                     /locus_tag="PK252_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14475.1"
                     /translation="MDNASDKYFDLKKKASEILTFNDDTIFSVSKYNENKANEKMKIY
                     LKNNYNIEISIN"
     gene            complement(27762..27998)
                     /locus_tag="PK252_06910"
     CDS             complement(27762..27998)
                     /locus_tag="PK252_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14476.1"
                     /translation="MREQIWALFCDLKYKSYLSSILVDKYQKWDRNVNIFIAFSSSSS
                     VASWIIWNKYQVVWSAIIVISQLLTVIKPYIPFF"
     gene            complement(28044..28658)
                     /locus_tag="PK252_06915"
     CDS             complement(28044..28658)
                     /locus_tag="PK252_06915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOT14477.1"
                     /translation="MHIAIGGNIGSGKTTLAGLLAKHYNWEPHYENIDHNPYLTDFYE
                     DMQRWSFNLQVYFLKTRFEQLITLQKKKETVVQDRTIYEDAHIFAPNLYAMGLMSSRD
                     FETYKSLFDIMSALITPPQLLIYLRASIPVLVQQIQKRGRDFESSIRLDYLQRLNERY
                     EAWIENYNLGKVLIIDVDNYNFVDNPEHLSYVLDKINIQLNGLF"
     gene            complement(28761..30290)
                     /locus_tag="PK252_06920"
     CDS             complement(28761..30290)
                     /locus_tag="PK252_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOT14478.1"
                     /translation="MPIFEKLFKAITFSRLKKIEYCALHANEIQEMTFKGLIDRAKKT
                     EFGKTFHFSAIKTPESFASKVPVQHYEELYPWINKIRQEEENVLWPGKIDWLAKSSGT
                     TNDKSKYIPVTWESLHKCHYRGGTDSIILYLHNNKKSKLFCGKTITLGGSHKIDSLGN
                     NIKAGDLSAIMIQNIPTLANIFRTPPKKIALLSEWNEKLEKLSNHIIKENITAFAGVP
                     SWNMVLMKRVLEITGKRNLLEVWPNLELFIHGGVNFSPYQNSYKNIIPSENMHYMETY
                     NASEGFFAIQNDLQDSSMLLMTDYGIYYEFIEMTNYYQGKKNTIALADVKTGVNYAMV
                     ISTNGGLWRYIIGDTVMFTSTNPYKIKITGRTKHYINAFGEELIVDNADHAIAEVSAK
                     LNIRINDYTAAPVYMSEKSNGCHQWLIECDKKDINPHIVAQILDEELQKLNSDYQAKR
                     YNNITLTIPQVTILPTGTFMKWFEINNKLGGQNKIPRLSNDRKHIESILKLLPSLEKK
                     A"
     gene            complement(30367..31245)
                     /locus_tag="PK252_06925"
     CDS             complement(30367..31245)
                     /locus_tag="PK252_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14479.1"
                     /translation="MEKKKITVKHYLNLRAKEKTFQREKFFPLYIQIIVNGKKAQIKS
                     RIHEFLKIYRSDIERITQNNAEFYRLILDGYFSDKLLDNIEKRKLFPLYQLLQDEIAV
                     LKRIIASMRPFDNPKFTLYNFGAEYQVHTTEITRIFDNYIKENFRNELQAIFLRTIDQ
                     DENRELFKIVNFYINYLNWENSFSPLYQSIMEIIPEEMHIIENQLTKELFISIKAYLT
                     YLSKVNIVNRLFERRQEGHITTLSYLDWQTDIKDFVHNEFKKAVGEQKALEYLISLDA
                     ILQKAIKTVSVEAKTE"
     gene            31460..33010
                     /locus_tag="PK252_06930"
     CDS             31460..33010
                     /locus_tag="PK252_06930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT14480.1"
                     /translation="MTQEELFKKLISHCKEYGFIFPSSEIYDGLSAVYDYGQYGSELK
                     NNIKKYWWDSMVLLNDNIVGIDAAIFMHPRTWEASGHVGAFNDPLIDNKDSKKRYRAD
                     VLIEEHIAKLEGKIDKEVEKAAKKFGEAFDKNQFITTNGRVLEIQKEIDTVRARFVAA
                     LEKPDLDAMRQIIVDCEIVCPISGTKNWTDVRQFNLMFDTKIGSVSDEANVIYLRPET
                     AQGIFVNFLNVQKSGRMKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     SELDWFDKWKEKRLRWHLAMGINPEKYRFHKHDKLAHYANAAYDIEFQFPFGFKEVEG
                     IHSRTDYDLGRHAQFSGKKITYFDPELNQSYVPYVIETSIGVDRMVLLALANGYAEEK
                     VVKEDGTTDERVVLRIPPALAPVKLAVLPLVKKDGLDEKAQQIVKALRFEFNCQYDEK
                     DSIGRRYRRQDAIGTPYCITVDHQTMQDDTVTIRNRDTMQQERIPIAGLQKYMGDKVS
                     IRSMLEKI"
     gene            33090..35708
                     /locus_tag="PK252_06935"
     CDS             33090..35708
                     /locus_tag="PK252_06935"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOT14481.1"
                     /translation="MKNLKAAYPHLIAIAIFVLLASIYFAPHFKGYGLSQHDMQTAAG
                     MAKETVDFNKIENAESFWTNSAFSGMPTYQTAMNNKSNIPQLIESNIFVRFFDYSIGF
                     IIIAMISFYVLMLCFGASPWISIIGAIAYGFASINILYLGAGHVTKVHAIALMPGIIA
                     GLVYAYRKNLYIGAALVALFVCLHVSANHIQETYYLLFLIFTIIAFEFYRFYKEKLLV
                     SFIKITGIVAIAAIIGLLPAIIGVLLPYEHGKYTTRGKSELTINASKFEDKTNGLDNG
                     YIKQYSLGYGEVWSVAVPNIKGGASGYIGNDEKNLDKVSPEFKNYVASFSSYWGEQLF
                     SGGAFYFGAIIFLLFVLGVVFIKDKLKWAFFSISMLAVVLSWKFGFITDLFINNFPLF
                     NKFRDTKMMLVIAQLSFPFLGMLFLNELFSNKLDKKKLNYALIGTIGVFVIFYIMPTV
                     WFSFFNSQEKNYFDQQMAAYGNNPSALQQLEQFRIEIQNVRVDIFKADVLRSILFMLV
                     AGLFIYLFNAGKLAKKYLLIVLGVLILFDIWSVDKRYLNNKKENGEYRSWVTNESKAK
                     PYPASEADKAILQMELRQNPALSEKIGPAIEKLSTGNDNVFNHDELALFALRAETNYR
                     VFTLQDPFSNASVSYYHKSIGGYHGAKLKRYQDLIDFRLSKEYEAIVNAFNTKSDTAI
                     FKVINSGIPTLSMLNTKYIIYNPGAAPIVNPNALGNVWFVRNVKVVANPDAEILALDS
                     INPAQTAVVDKRFENFINTDIKEDSTAKIALVSYKANALVYESNTNTNQIAIFSEIYY
                     KDGWNAYIDGNPADYFRANYVLRGIQVPAGKHKIEFRFEPTIVRTARNISLAGSVLLF
                     LFIGGMLFFNYKKKKE"
     gene            complement(35710..36486)
                     /locus_tag="PK252_06940"
     CDS             complement(35710..36486)
                     /locus_tag="PK252_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT14482.1"
                     /translation="MTTGEARTLNESPIVSIITVVFNGEKVLPRTIESIRKQNWKNFE
                     YIVIDGNSKDSTLEILRQNEDIITYWQSEPDKGLYDAMNKGLKAAKGYYVCFLNAGDQ
                     LYDENTLKSIFEGLNPLPDIVYGETMIVDAEGKEIGLRRLKAPEKLTWRSFINGMLVC
                     HQSIYVKRELADSYNLKYKIAADYEWVLKALKKAKSIHNSKLVVTRFLDGGINKKNIR
                     RGLSERLRIMIKHYGFFLTTFNHLVIGVKFFWFLFKNKRF"
     gene            complement(36495..36908)
                     /locus_tag="PK252_06945"
     CDS             complement(36495..36908)
                     /locus_tag="PK252_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT14483.1"
                     /translation="MNRALIDTDILSYYFKGDPLVVKNFKKYLTEYDIIEISLITYYE
                     IMSGLLHKNAHKQLEMFNDFVDENFVVALIEESCKISSEIYSKLRISGESVDDIDLLI
                     AGVAIENEMTFVTNNESHFGRIPGLIIENWKTQEL"
     gene            complement(37204..38460)
                     /locus_tag="PK252_06950"
     CDS             complement(37204..38460)
                     /locus_tag="PK252_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT14484.1"
                     /translation="MLVVLINTSSKKGGAAIACRRLLKALNYNGTTAIMLVRDEAITE
                     NMVSTTHSKLKKWINFYRFAYERFFFFVREKSKSVRFLFSIANVGEDIHKRKEVKQAD
                     IIHFHWINEGFISITGIKEIFNQKKPVVWTLHDMWPFTGGCHHSGECNRFKQSCGECP
                     FLKYPSSHDLSYRILQKKKKAFAQANLTVVTCSQWLAGKARESALFANINVVSIPNPI
                     DTNVFKPLDKTEARKTFGLKENKKYLLFGAVNVKNYFKGFTYFRDALKVLIQSNPELS
                     GQLEIVILGRSDEQILREVPFKCNVIGSIHEESMMSQLYNAVHWFVTSSLQENLPNTI
                     MESFACGTPVVAFNVGGIPEMVEHKQNGYLAEYKSIDDLANGIKWAIYEVDHSQLSEN
                     ARKKVLDCYAEEVVAEKYNNLYKSLI"
     gene            complement(38467..41502)
                     /locus_tag="PK252_06955"
     CDS             complement(38467..41502)
                     /locus_tag="PK252_06955"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOT14485.1"
                     /translation="MKNFKRYNNISGWLVFAIAATVYLITKEPTASLWDCGEFIATSY
                     RLEVGHPPGAPLFMMLARMFSLFAGNNVALVASMVNSLSALASAFTILFLFWSITHIG
                     WRVAEKSMKSDTTHGILVLGSGFVGALAYTFSDSFWFSAVEGEVYALSSLFTAVIFWL
                     VLKWEENADQPRADKWLILIAYLMGLSIGVHLLNLLAIPAIVLVYYFKKYPYSLKGFL
                     TAIIISIIILAFVMYGLIQGLIILASKFELLFVNGIGLPYNSGIIFYIILLLGTIVYL
                     IIYSVKKHKRWLNLAILSITMIIIGYLSFAMITIRAVADPPINEGNPSNVFALLSYLD
                     REQYGDRPLFKGQYYNAPIVEVKDGEPKYVPLDGKYEIIGYDYDITYDSRFTTLFPRM
                     YSSDPQHVQSYHNWANIKGKLVDVTENGKVKKYVVPTFSENLKFFFSYQVGHMYMRYF
                     LWNFVGRQNDEQGNGILNGNWISGIPFIDAHLTGRQDNLPDIIKKDPSRNNYFFLPFI
                     LGLIGLFFLLDKSRRYFVVTVTLFILTGLAIVVYLNQTPLQPRERDYAYVGSFYAFAI
                     WIGLGVMGIFNFLKSKKDHLAKVITIIIICIFCVPVLMAVQNWDDHNRSGRYLARAYA
                     YNYLQSCAPNAILFTYGDNDTFPLWYLQEVEGIRTDVKVVNTMLLNGDSYIDQMKKKA
                     YLSDPLPISLNHKQYMEGTLDRVYVLDYIKDSISISDAIKFVASDDPRTKTIKGYDEK
                     IEYIPCKRLALPINKENLAKGGYIEKSYIDKAPSSITITIPENYIIKSQLIMLDIIAQ
                     NQWKRPIYFLANYASGTCWLNNYLQRDGFAYKLMPVKNTSENELSLNADSMYNKLMNV
                     FDYGNMQSPKVYLDHFHKQTLSIIRFRSVFAMTAEALNNEGKKDSAIAVLDKCEKLLP
                     PSKMPDDMGSLYIANCYLEAGATDKGNAVIERFFDVTYKQMNYYASLRPGFRSMIKNE
                     MEENIQYLMQMQKLSEYYKLPIASKISSILGLMQIQTNE"
     gene            41626..42123
                     /locus_tag="PK252_06960"
     CDS             41626..42123
                     /locus_tag="PK252_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOT14486.1"
                     /translation="MNYKLHFKPITDEDYFEIKEIYDYYIHHSTATFHINSLSIQELK
                     EFIFENHHLYPAYMIKCDGTTAGYCYLTYFKKREAYDRSAEVTIYLKPEFCNKDIGHK
                     ALEHLEKEAHKRHLKNLVALITGNNYQSIKLFEKSGYVKVAHFKNIGEKMGEVLDVLA
                     YQKEI"
     gene            42138..42479
                     /locus_tag="PK252_06965"
     CDS             42138..42479
                     /locus_tag="PK252_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOT14487.1"
                     /translation="MKKDKDRVNVVYSTNPSFQYEYNVGPEAITLPPQQQNLRVKNDR
                     KQRAGKTVTVVSGFVGSKEDLEALGRTLKSKCATGGSVKDGEILIQGENADKVVQILL
                     QAGYKVKKSGG"
     gene            complement(42481..43173)
                     /locus_tag="PK252_06970"
     CDS             complement(42481..43173)
                     /locus_tag="PK252_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOT14488.1"
                     /translation="MQGDLISYLKHRYEETQKRHTGPGPVITISREYGCPAKLIAEKL
                     ANELSAKHDEQGHTHLWKWYSKEILEESAKELQMDPSKIKYVFEYEKKSALEDFFGSF
                     GQYYQSDKKIRATIGRVIREIGENGHAIIVGRGGIAITRDIADSLHINLVAPLEWRTV
                     RISQRYDLTLENARQTCIDIDKKRAEFRDFFQGKNNDYTSPDLTINCMSFTVDEIVKI
                     LVKTVETRKLIY"
     gene            complement(43332..43787)
                     /locus_tag="PK252_06975"
     CDS             complement(43332..43787)
                     /locus_tag="PK252_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOT14489.1"
                     /translation="MIFDNLNHLGKYSHVMPEIEKVLDFIQKNDLSKMANGKYEIDGA
                     NVFASINEYKSKPEQDVKWEAHIRYADIQMITKGSEKIGFAYQPEMDIIDPYNGEKDI
                     MFLSGNGSFLNLKPGTFAIFWPRDAHKPGVMNTAVPDDIKKIVFKIKVA"
     gene            complement(43795..>45541)
                     /gene="dnaG"
                     /locus_tag="PK252_06980"
     CDS             complement(43795..>45541)
                     /gene="dnaG"
                     /locus_tag="PK252_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT14490.1"
                     /translation="FIMEHENLSYYDALRYLAKKYNIPIVEKELSPEEAAQRTEREAL
                     LVVTDFAAKFFRKTLNNHQEGIAVGKAYFRERGIRDDMIEKFQLGFSPTQRDALLNEA
                     LRAGYQLEHLIKAGLVAEKDNYRYDKFAGRVIFPILSISGQVIAFGGRTLRTDKNVAK
                     YLNSPESEIYHKSNVLYGLFQAKKAIEQQDKCFMTEGYTDVISMHQVGIENVVASSGT
                     SLTINQIRLIKRFTNNLTVLYDGDMAGIKAALRGIDLILEQGMNVRVLLLPDGEDPDS
                     FSKKHSASEVLDFIAKNESDFVLFKTKLLLDEAKNDPIKRATLIADIISSIAIIPNNI
                     VRSEYIKECAHILHTDEAVLYTEVMRVRRKKTEGNIAAPSPIVIEEKRTDKPLNQTNE
                     FEFLEREIIRLLLNYGNHILFEQTEIKGKQKQVHIVTVDHYIIDEIRKDELELQHPIY
                     RQIFEEYEINCLKNPEDATAYFLRHQDKEVHQVVADLLTKKYNLSKKLFARGAHLVIE
                     TEESKLPDLVRSVITEFKNKKVISFIKQIKLQLLEAQEKEDVDRIIALQEQMQQLNIL
                     KKAFAETLGNRIIIK"
BASE COUNT        14002 a         8150 c         9074 g        14315 t
ORIGIN      
        1 actggtatcc ctccgcgata ctattcgttc atcatcctta tgtcttcttc attcagcgat
       61 ttctcatcgg tgtcggtaca gtaaatatcc acgttgaaat tatatggtct ttcaaaagac
      121 ttggaattac aacgaagaga cttgtttttg aaaactttgt ccatatatat agcccagaca
      181 ggaagtgcca tgttagcccc ctgccccatg tcaatattgt caaaatgtac agcgcgatct
      241 tcgcctccaa cccatacccc tgtgaccaag tcgggggtaa agcccataaa ccatccgtct
      301 gagtggtttt gcgtagttcc tgtttttccg ccaataggcc cggtgaattt atatccataa
      361 cgcaaacgac aagctgtccc ggcattgaca actccctcca tcaggtttac catcaggtac
      421 gcggtttgtt cactaatggc ttcggttttt tcctgcgtaa atgtggccaa cacattgcct
      481 gttttatctt caatacgggt tacaaacatt ggcttaacat atataccctt attggcataa
      541 catccataag ctccggtcat ttcatataag gtaacatctg aagttcccaa taccaaagat
      601 gggtaaggtg caatatagct ggtaaagccc attttacgca tgatatctat cactgatggt
      661 gggttaaact gcttcatcac ccaggccgag ataaagttca cagaattggc caagccccat
      721 tttaatgtaa ccatctttcc ttcgtagtcg ctacgtcctg agttttttgg gctccagatg
      781 gtatctccga gtataaatgt tacaggcaca ttggggactt tgtcgcatgg cgaatagcct
      841 tcctgcatgg ccagggtata tataaaaggt ttaatggtcg aaccagcctg acgcttacta
      901 atagtaacat ggtcatattt gaaatacttg taatctatgc ctcctacgta tgcttttaca
      961 taacctgtat gcggatccat agacatcaag ccggtttgta gaaaaaattt ataataacgt
     1021 atcgaatcta atgggcttaa aatagtatcg cgttcgccat tccatgtaaa aacacgcatt
     1081 ttgtgttttt tcttgaaatc tttcataata tcctcaaaac tccaaccttc gttacgaagc
     1141 acccggtaac gctcagtgcg tttcacggcc tggtccatta tctgctggcg ttgatcagca
     1201 gtcaggttgc tggcaaatgg agcatcatgg cgtcctttct tttccttaaa aaaagcaggc
     1261 tgcaaatatt tcccaacatg ttcgtaaaca gcatcttcag catattgttg cataactgaa
     1321 tttatggtgg tatatatttt tagcccgtca gaatatatat tatacgttgt accatccggc
     1381 ttaacatgtt tattgcacca tccgtaaagc ggattgttac accattctac cgaatcgcgc
     1441 ttataatcct ctatatcata gtaatttttt ttctgaggct tattggctgt gagtgttgtg
     1501 cgcaaatatt ccctaaagta tgtagcaagc ccttctgtat ggtcttgtgc tttgtaatcg
     1561 actattaatg gtaagttttt taacgaatca cattgccttt tatttataaa atcatacttt
     1621 tctaactgag ataacactac atttctccga cgcaaagctc tttcaggatt ctgaaccggg
     1681 ctataccttg taggagcttt aagcatcccc accaacattg cagcttcctc aatatttaaa
     1741 gagtctgtag atttattaaa atatgtcttg gctgccatct taataccata agcctggcct
     1801 ccaaaaggta ctgtattgag atacatggtg attatctcct ccttggtata gttgcgctct
     1861 aacttaactg ctgtttgcca ttctttgaac ttggctatag caagcgatgc cgatttcgaa
     1921 agaaagttat tatataaaga ggtgtccctg ggaaacagat ttttggccaa ttgttgagta
     1981 atagtacttc cccctccgct attactctgc aaaataattg ttttgaaaaa aacccgtgcc
     2041 aaccccctga tgtcaatccc cgaatgcttg taaaaacgga catcttctgt tgctatcaat
     2101 gcattgataa cattgggcga tattttatca tagtccacaa acgaacggtt ttgatagtaa
     2161 tatgtaccca acatgacacc atcttcagca ataatctcac tggctatgtt attgttagga
     2221 ttttccaaat cctcaaatgt tggcataaac ccaaatgccc ctatcgaaat taaaataaaa
     2281 attaatgcta ctaaaaaaaa aggtgttaca aataataccc aaagaatttt cttgtactta
     2341 ttagttttat caacggtttc agccatatct tgtcctttct gtttgtgaca aaagtagcaa
     2401 ttatttgttt taaaattttg aacttacctt tctatttatt ttactttgtg ctttcttttt
     2461 caaaaattta cagacagtta attttatatt tattatggta gaaaatcttg tcatcgttga
     2521 gtctccggca aaagccaaga ctatcgaaaa attccttgga aaatcattta ccgtgatgtc
     2581 aagcttcggc catatccgcg atttagccaa aaaaaacctg ggtatagata taaaaaacaa
     2641 ctttaacccc gactacgttg tttcggacga taagaaaaaa atagttgccg agctgaagaa
     2701 aaatgttaaa gaagccaaaa tggtttggct ggcttccgat gaagaccgcg aaggagaagc
     2761 aatagcctgg cacttggctg aggtgttgca gcttgatgaa aaaaaaacca aacgcattgt
     2821 tttcaacgaa attaccaaag aagccattca gaatgctatc aaaaacccca gaaatattga
     2881 taaaaacctt gttgatgcac aacaggcccg ccgcattctc gacaggttgg ttggttttga
     2941 actatcgcct gtactatgga aaaaagtaaa gccatctctt tctgccggtc gcgtgcaatc
     3001 tgtggctgtc aggcttttag ttgaccgcga gcgcgagatt attgctttta agccatcttc
     3061 atcatacaaa actgtggcgg tgtttgaagt tgtggacaat aaaggaaaaa aggttttatt
     3121 agaagctgag ctggatgaaa agtttaatac taaagctaaa gcccttgaat ttttaaaggc
     3181 ttgttcaaaa gcccagtttt ccattgaaga tgttgttaca aaaccttcta agcgttctcc
     3241 ctctgcacct tttacaacct ctaccctgca gcaggaagcc agtcgaaaac tcggtttttc
     3301 agtatctcaa accatgacta tagcccaaaa gctttatgaa gctggtaaaa taacttatat
     3361 gcgtaccgac tcggttaact tgtccgaaac cgctttaaaa gcctgcaaag aagtgattaa
     3421 caaagagttt gggaatgaat atcacaacct tcgtcaatac aaaactaagt caaaaggagc
     3481 acaagaagct cacgaagcca ttagacccgc ttattttgac caggaaaccg tacaaggctc
     3541 tgctgctgag aaaaaattat actctctaat ttacaaacgc actttggcca gccagatgag
     3601 cgatgccctt ttcgaacgta ctactgtatc tatcaaagtt gaagggttgc catatcattt
     3661 tgttgctact ggcgaggtgc tcaaatttga tggtttcttg aaaatgtaca tcgaatcaaa
     3721 tgatgaagaa agcgaaagcg ataacctcaa aattttgcct cctttaacca aaaaccaacc
     3781 cttaaatgtc aaaaatatta cctctacaga aaggttcacc catcatccac cccgatatac
     3841 cgaagcaagc cttgttcgaa aactcgaaga gctcggtatt ggccgtccat ctacctatgc
     3901 tcctattatt tcaactattc agcaacgtga atatgttgtt aaaactgaca gagatggatc
     3961 tgaaagaaat tttgatgttt tgaagctcga aaataaaaat gttaccgagc tgacagaaaa
     4021 agaaactttt aacgctgaaa aggcaaagct tttccctact gatataggga tattggttac
     4081 cgattacttg gttgaccatt ttaaagacat catggactat aattttaccg ccaacgttga
     4141 aaaagatttt gacgaaattg ccgaaggtaa aatgctttgg accaaaatgt tagatgcttt
     4201 ttatgcccca tttcataaaa aagtggaaat ggccattgct actcgcgaaa atactaaagg
     4261 tgaccgtttg attggtaatg acctggtttc tggcaaaccc ataattgccc gtatcgggcg
     4321 atttggccct atggtgcaaa ttggtcagtc tgacgatgag gaaaaacctc gttatgcaaa
     4381 gttgcaacca ggtcaatata ttgaaactat tacccttgaa caggccttga acctttttca
     4441 actccccaaa aaacttggct cttatgaaaa ttatgaagtt agcgttggca ttggacgctt
     4501 tgggccttat atattgcacc atgccaagta ttattctttg gtcaaaggcg ttgacgaccc
     4561 atataacatt actttacagc gtgctattga attgattaac gataaaagag aaaaagataa
     4621 aaacaaggta atcaactcct ttgagtacca aaagtcagaa attcaagtac tcaacggccg
     4681 ttttggccct tatatttgtt ataacaaaga aaactataaa atccccaaaa atactgatgc
     4741 tacaaaactt actgcagaac aatgcattga tattatccaa aaaggagctt caaccaccaa
     4801 aaccaaaaag aaaagaaaat aataatgttg ctgtcagatt accttctccc agtatctctt
     4861 gataagccca acgatgaggt tttgcaaaac aaaaactcgt tttgcaggca tattgatatt
     4921 aatacagcca ataagcctgt tgatttagat aaaaaaaaat acaagttggc aattattggc
     4981 gtacccgaat accgagggtg tggtaatgaa ggagtagtta atacccctaa tgctatccgt
     5041 tctgagctat atcagcttca ttatttcgaa aaaaaaatcc agttggctga tttgggcgac
     5101 cttaaaattg gaaaagaact caccgatact ttttatgccc ttcgtgaaat tgttctcgaa
     5161 ttgcatgcca gaaatattgt accgattgtt attggtggct ctcaggatct gacttatggg
     5221 atgttcctgg cttttgaata cctgaaagtc ccttacaacg ttgctactgt cgatttcaag
     5281 ctcgatttgg cctttgacag ttatacccct attaactatc aaaattatct gaataccatt
     5341 atcttagaaa ataaatacct gtttgaattt tttaatcttg ggcaccaagc ctgctttagc
     5401 aactacgatg gcaacttttt gttagatact ttgtcgcacg aaaaagtcag gttgggtaat
     5461 atgcgaaacc agaccgaaaa gctggaacct tacctcagag acacccattt gctgagcatt
     5521 gatttttcag ccattaaaca tgccgatgcc cctgcacaat tagtttcatc tcctaatgga
     5581 ctttatgcag aagatttttg ccagtgtatg cactatgctg ccctgagcga gaacattaaa
     5641 gctgttgggt tgttcaatat agatgcctca aaagattacc aagggcttac ttcaaagctt
     5701 gcagcccaag gaatttggta ttttattgaa gctttttcgc gccgaaacat tgaaaaccct
     5761 tccaaaagct cttcaaagtt taaaaaattt tcagttactt taaacgaaga cacctctatt
     5821 gtattcctta aaagtaaccg gacaggacgt tggtggtttg aaatttctga caaaaaaaat
     5881 caaaaaattt attttgcctg tgctgagaat gactacctga cagccattaa caatgatatt
     5941 cctgaaagat ggctaaaact ttttaaaaag ctaaactagc ttcttttttc tgctttgtag
     6001 caatacacaa tatttgaaaa attattgcgt ttgcatattc aggtttattg aagaaatatt
     6061 tacaatattg acaatatgac tgagttgttt caatatttat ttttttatat ttgttgcaaa
     6121 atactatttt tgttaattga tataaattaa aactcatata tctgttggat aataatattt
     6181 ttaaaagtaa tttatgtttt ctggttcgaa ttttttatgc ttctaaaggc atagcagttc
     6241 gaaaggggct attttatact gttttatttt tacttttggg gccttctttt tttactcgtg
     6301 cccaagaaag ccagtttact catttcatgt ataacaacat ggggataaac cctgggtatg
     6361 caggaagtac agaaatggtt ggaattaaca ttatccatca gaataagctt atgggttttg
     6421 aaaaagctcc ccgacacgat attgcaagca ttgatatacc ttttactctt ttcgaaaaga
     6481 gccatggtgt tggccttaac ctcataaata gttcaatggg ggtaaataat gacatcggaa
     6541 tgcaacttgc ttatgcattt caacgtaaaa tggccattgg tgacggaaag ctcggtattg
     6601 gtatctgcgg cggatttaac agatctacat tcaaagccgg agatttagcc agtggtacag
     6661 atcctttagt ccctaccaca gaagctactc caatggtttt tgattttggg acgggcgttt
     6721 tttacaatag tgaaaaaata cacatgggta tatcagtagc acacttaata accggagaac
     6781 gtgattataa aatagcagca ggggatgtta tgccgcttca acggacgttt tatgtaacag
     6841 caggatacaa ctatcagttg cctaatccta tgcttatgtt atgtccttca tttttggtgc
     6901 aaactagtgg gaaccttact gcagtgagct ttaacacaaa tattgtatat aataatagaa
     6961 tatggggagg cttttcctat tccgcccaaa attcagtttc agccatgttt ggacttgaat
     7021 tattacccgg ggtaaagttt ggggcatctt acgattatag cgtcaattac ttacgaaatg
     7081 gaactggtgg cagctttgaa atattcgcaa attatagttt taaactaaaa aaagaaaaaa
     7141 taccacaacg atataaaagt ataagatatt tataagaata ttttattttt gaaaaattat
     7201 cattattttt agaaaagtat tgggcaaaaa aataataaca aattgttatg agaaaatttg
     7261 tgtatctcag tgttgtaatc cttgtcttag caggatgcgg aaagccatca gacacgggcg
     7321 aacttacagg agtgagtgat ggtagaaagt attttgaacc ggatccatac ggaatggttt
     7381 ttatccctca agggagtttt aatatgggcc ctagcgatca ggatgtagcc tgggctcaaa
     7441 atagcttatc caaaacagtt tctattgctg cattttggat ggacgatagt gaaattacca
     7501 acgatgaata tcgtcagttt gtcaaatggg ttcaagactc aattatgcgc cgtatgcttg
     7561 ccgctcaaat tgaagatttt gctattgctg aagatgaact tggcaatcct attgatcctc
     7621 catttctaaa ctgggaaatg gaaattgacc ctcaggacga ggaacaaagt ggcattatta
     7681 atgaacttta cctgacagaa caagaacgtt tttacagaag aaaagaaata gatacccgca
     7741 aactcaatta tgaatactat tgggtggact taaaacaggc tgcgcaaaaa gggaacaggt
     7801 ataattttgc tacacaggct tatgaaggta ctgtaattaa cagcaaaggt gaaagtgctc
     7861 aaattatcaa ccgttcatct tttgtattac aagattttat caatgtttat cctgatactt
     7921 tgtgttgggt ggcagatttt acctattcat ataatgaacc tatggccaat atgtacttct
     7981 ggcatcctgc ttacgataat tatcctgttg ttggtgtttc gtggaaacaa gctactgcat
     8041 tttgtatttg gcgaacacaa ttattgaata caacactcca aaagaatgga gaattattcg
     8101 ttcacgatta ccgtttgcct agtgaagcgg aatgggaata tgctgcaaga ggaggtttag
     8161 atttgtctat gtatccttgg ggtggtatgt acacccgtaa caatgaagga tgcttccttg
     8221 ctaatttcaa accattgcgt ggtaattata cagatgatgg aggtattaca actaccaggg
     8281 taggaactta cgatccaaat gaatatggac tttatgatat ggcaggtaat gttgctgaat
     8341 ggacttccag cgcctttgac gaatcagcat atatctatac ccacgacctg aacccggatt
     8401 acaaatacaa tgcattgcca gatgatgctc cggtattaaa acgtaaagta attcgtggtg
     8461 gttcctggaa agatatagga tatttccttc aggttggaac cagaagttac gaatatcaag
     8521 acactgcaaa aagctatatt ggatttagat gtgtaagaac atacttagga aatagtaacc
     8581 aataatcccc ttttaattat gaccttatct gaaatagtac aatcatccgg gtggaaaaat
     8641 tttatgtcca aactttacgg gtatggtgca tctgtagtaa ttattggtgc aatgttcaaa
     8701 attatgcact ggcctggagc aggttttatg attgtggctg gtcttagtac cgaagccatt
     8761 atctttttct tttcggcttt tgaacctttg cacgaagaag tggactggac acttgtatat
     8821 cctgaattag caggtattac agatgatgac gaaatggaaa attacaaaga aagcgttaca
     8881 aatagtagag gaaatgtagt acttgaaaaa tttgaaaacc tttttgaaca atcttctttg
     8941 agccctgaaa ccctcgaaaa acttggtgaa ggattcgcta aattaagtga taccaccagc
     9001 aggctttctg atatttcaga agctacagct gctactcagg catttgcttc aaattttcgt
     9061 aatgctgcac aatcattgga ttcaatgaca tctctttatt ctgaaaattc tgaggcactg
     9121 agcagttcta taagttcact ttcaaaaacc tatcaaaaaa ctgccgagat tattgaaaag
     9181 accggggtac aggtttcagc tcaagttgaa aaaagtggat tagatatggc aaattcatat
     9241 caaaaacttt cggagtctat tgcttcttat tctgcttctg taaataattc aggacagaat
     9301 tatcaaaatc aacttgaaaa aataaataag aaccttggag agctcaatac tctttacgaa
     9361 ttacagttga aagaaactaa tgattatgta aaacacaatg agcaattata tgccggattg
     9421 agcaatatta taggtaacct gagtgaatct gttgcagaga cacaacgtta ccgtcaagag
     9481 atggccaagc tttctgaaaa cttatcatca ctgaataaca tatatggaaa tatgttgtca
     9541 gccatgaatt attcaaaaaa gtaatttaga agtttatggg ccacggaaaa gaaacccccc
     9601 ggcaaaagat gattggaatg atgtacctgg tactaacagc cttgttagca cttaatgtac
     9661 agaaagaagt tttgaatgcc tttgttattg ttgatgaagg tatgaccaaa atgaatgaaa
     9721 attattccaa gaaaaatgag gaaatgtatt ttgcttttga tcaggctaat gcccaaaatc
     9781 ctgctaaatc taaaaagtgg cgggatattg catatacagt taaacaaaaa tcagaagagt
     9841 tatacgaaaa tattcaggat ttaaagttaa aaatattatc tactgcggga gaagttgaag
     9901 ctgttaaagg cagggaaatt aataccatgc ttataaatgg caaagaaaat actgatgtgc
     9961 ctgcacaggt aatgattgtt gaaggcaacg ggaagaaact taaagcgatg attgaggact
    10021 ataggagata tttgattgaa caaatagatc ctaaggctga agctttaatt aattctcttg
    10081 aaacagggct ttcaacaaaa gatcctgaac ctgttggagg aaaaactgag tcatgggaaa
    10141 gtgaacattt tgagcatttg cctttagcag gggttttaac tattatgaca ggtcttcaaa
    10201 gtaatgtaaa aaatgctgaa gccgatgtgt tgaggtactt atatactatg attgataaag
    10261 ggtcatttaa atttacaagt ctcgaggcta ctgttatagc aaactctaat tatgtcatcc
    10321 aggggaatga gtatcaggct aaagtcttta tggcagcttt tgatacaacc caggatcctg
    10381 agatatattt aggcccttgt gattctgtcc ggactgaaga aggatatgta tatacagaga
    10441 agaaaggctt caattatgga gctcctcttc ctgtcaaaaa agggaaaggc atttattcag
    10501 tcagggcaac agcgcccgga gaaaagaact tagttgggat tattaaattg aaagctcctt
    10561 cagggggaca agatattatc cgtccgttta agacatcgta tcgtgttgct gaacctatgt
    10621 tagtggtttc gccaactaag atgaatgtat tttatcttgg agttgataac cctgttgaaa
    10681 tttctgttcc tggggttgaa gctgaaaaga tttttccaag tattagtaat gggactatta
    10741 ccaaaagtgg aaaatctttt attgtaagac catctaaatc tggaattgct caaattaata
    10801 ttttggcaga aatagataag caaaagaaac aaatgggggc aaaagagttt cgggtaaaaa
    10861 ttgtacctga ccccgttgca aaagttgctg ggattaaagg gtctggagtt atagacaaaa
    10921 atgttctcct tgcacaaagt ggtattgtag cagaaatgga aaactttgat tttgaccttc
    10981 agtttaaagt cactgaattt aaagtttctg ttaacgttgg aggtttttct aatgataaaa
    11041 cctcaaaatc aaataaattt accccagagc agtttaattt gataaaagga gcaaatagag
    11101 ggcaaaaagt atatatagaa gacgtaaaag ctgttggccc tgatggttcg atcaggttat
    11161 taggctctat tgcattaaca ttgaagtaaa atagaaacaa ttaaataaat ataataatga
    11221 aacgagcatt attattcata gcagggttga gtattttgtg ttcggcacaa gttgttcagg
    11281 cacaagataa caaaacagaa gtatatgtca aagaacatat acctaacaag aagccggtac
    11341 cttatccata catgagagag gcagatgtga tgtgggcaaa aataatttgg agggtaattg
    11401 atttgcgtga gaaaatgaat atgccgcttt attatcctac caaacccatt gataaaagga
    11461 tgaaccttat tacgttaatt ttgaaagggc ttgatgatga tagtctggga attcatgctt
    11521 atagtacaga tgatcctctc aacgagttta aagatcctcc attaacaaaa gaggctatta
    11581 atgagcagat gggtgcaaaa gtggacactt taaaggttac agatgttaat accggagatc
    11641 ttgaaacaaa aattattacg cgtgaacggc aaatagatgc tgttaagcaa attatgatta
    11701 aagaaaagtg gttttttgac aagcaacatt ctaccatgca ggtgcgaata cttggtattt
    11761 gccctattat tgtcagaaac aaagaggtta atggtcagat tactgatgaa atagagaaaa
    11821 agttgacttt ttggatatat tatccagaaa taaggaacct ttttgccagt tatgagattt
    11881 acaatcgttt taatgacgca cagcatattt catttgacga tttgttttta caacggagat
    11941 tttcaagtta tatatttaga gaatcgaacg tatataacaa ccgaagtatt ggtgagtata
    12001 ccagtggtat agaaacgttg tacgagtcag aacgcattaa agaatcattg tttaattacg
    12061 aacacgatct ttgggaatat taaaaagtta aatttattca ttatggcttt ggtattaaat
    12121 atcaaagcca ttttttattt aacatatgct tagtatagaa aagaaaatat taacggttgg
    12181 tgcattattg ccagaattgg cacgattacg ttttcaagat aagaagattg tttttactaa
    12241 tggatgtttt gatatccttc accgagggca tgtagattat ttatcaaggg cggcagaatt
    12301 gggagatgtg tttattgttg ggttgaatag tgatgcctct gtatcaaggt taaagggttc
    12361 aacacgtcct atacaagatc aatattcacg ttcaataata ttagccgctt tacaatgcgt
    12421 tagttatgtg atattgtttg atgaagatac gccttacaat ctgattaagg ctgtacagcc
    12481 tgatatactt gtcaaaggag gggattatac tgaaattagt aaaattgttg ggtttgatat
    12541 tgttactcaa cgaggtggga aagtaatgac cattcctttt gtcgaagggt tttcgactac
    12601 aggtatctta gaaaagataa gcaaattgta attgtgacca ataggttata aatccacacg
    12661 aaaagccgcg aaaaattgaa aatattttag catctttgtg ccaaaataca tttatttgta
    12721 aatatctgtt aatcaataat ttaaaaaaat ggaaaaatca gttgcaattt tatgtgcggg
    12781 agggccagct ccaggtatta ataccgtaat tggttctgta actaaagtat tcttaaaaaa
    12841 tggttacaaa gttattggta tccacgaagg gtacaaaact ttgtttaaag gcgaacctaa
    12901 tgttgagttt ttagattttc aatttgcaga caacgtatat agccgtggag gttcagcatt
    12961 gcgcatgagc cgctacaaac caaaagacga agaatttaag cctgattttt ttgaaagatt
    13021 caatgtaaaa cttttggtaa ctattggtgg tgatgatact gcttctactg ctgccaggtt
    13081 gacaaaatat cttcaatcaa agaaccttga tgtaaaaaac atccacgttc caaaaaccat
    13141 tgacaatgac cttccattgc cagaaggaat ccctactttc ggatatcatt cagcaaaaga
    13201 agaaggcgtt cgtttagcta caactattta tgaagatggc cgcaccagtg gtacatggtt
    13261 tgttgttgca gctatgggcc gtgaagcagg ccaccttgct tttggtattg ggacagctgt
    13321 gcattatcca atgattatta tcccggaaat gttcaacaaa acccatgtaa cttttgaaaa
    13381 gattactaat ttgattattt cttcaatttt gaaaagaaat atcatgggta ttgattatgg
    13441 ttgtgctatt gtaagcgaag gtgtttttca ctttatgagt gatgaagaaa ttattgcctc
    13501 tggcatccag tttacttatg atgaccatgg tcaccctgaa cttggaaacg taagtaaatc
    13561 acacattttc aattatttgc ttcaaaacaa attgaaagca ttgggcctta aggtaaagag
    13621 ccgtcctaac gaaatgggat acgaacttcg ctgctgccgt ccgatagcat ttgaccttgc
    13681 ttatgctaca caacttgggt taggcgttta caaactgttc agcgaaggaa agagtggttg
    13741 catggttact gttgaccatg tgaacaacat cacacctctt tatttgaaag atgtttcaga
    13801 tgaaaaagga aaagttatcc ctcgtttggt aaatattgat tcatcaaggg tacaaatggt
    13861 ttttgaaagc aacctgcatt atattgcccc tgaagattac gaagctgcta aaaagtttgt
    13921 taagaaccca gaagaatttg acttcaagaa aattcttaac tggtaataac acttttatag
    13981 aatacaaatt caaaggcctt catcaatgga ggccttttta ttttctggac aaactattca
    14041 cttatagatt ccttatttat cttgttggag gattgagata agaaagttat atttgttgaa
    14101 caaaatttga taaaaatgat actacttgat gctaccacag ttgctgtgac aaaatcagaa
    14161 tctatgattg atatcatgct gaaaggagga tggattcttg tccctatctt tctaatgtcc
    14221 atttttgctg tttattatat ggtagaaaga tacactgtgt ttcaacacgc ccttaaagtt
    14281 gataaaaata ttgtagatga tatatgtggg catataaaac atggtaattt agctaaggca
    14341 catgaattag ctagtgggta taaccatcca attgggcggg taatcgttgc tggtttaagc
    14401 aatccggcaa ggaaaattga agatatagaa caaattatgg ctgaagagac aaaaaatgaa
    14461 atatttattt tagaaaaaaa tttaggttat ttaggttcaa tagctaccat agccccaatg
    14521 tttggttttt tggggacgat tttcggcgtg ataaaaattt tttacaacat atcattggct
    14581 gataatatta gtatagggct tatagcagga gggttgtacc aaaaaatggt aagctctgct
    14641 gcaggtttga cagttgggat atttacttat gccggttatt attacttgaa caacagggta
    14701 gatgtatttg taaaccgtat ggaaagaaac ctgtcaccag ttgtcaatgc catcagggat
    14761 agcaggttaa gttaaaaaat caaggcgatg aagatagaaa gaagaaaaag caggacatcc
    14821 gaagtatata ccgcttcgtt gaacgacatc atgttcttcc tgttgttgtt ctttttgatt
    14881 atttcgacca tggtaaaccc ttcggtcatc aagttattgt tgcccaaagc ttctaattca
    14941 gagcagactg tttcaaaaca aaccatcaac ctggctgtaa ccaaggattt gaagtattac
    15001 cttaacgatg aagaagtccc ttttgataaa ttagaaataa aacttaaaat attgatgaac
    15061 agggcaaaag agactactgt tgttttgagg gctgataata ccattaccct tcaacccgtt
    15121 gttgatatta tcgatttagg aaacaaacta aagataaaag tcattttggc tacccgaaaa
    15181 gactgatgcc agaaagcaat aaacagcaag gaaaaaagaa aatatttttt caagaagtcc
    15241 gttatttcta atatttagca aaacaaaaca tgaaggccat caaattatta ataattgccg
    15301 ggctattgct atcggttaac ctttattctc agaaaataaa gagttttaaa gttgaagcat
    15361 tcaatcaact tgaagtatat ggtaaatttg acattgaact aaaaaaatcg tcagagtttt
    15421 cagtcaaact gtatagtgac aacattgatt tatcaaaagt tgatgtggtt gtggaaaaca
    15481 aaaagatgaa aatcagtgcc ctttccgggt tgttcaagaa aaacagtaaa gtttccgtga
    15541 ccattgaata cgattcgctt aactctttat tggttactaa tggggctgaa atatatagtt
    15601 caaagcccgt gcaagtaaat aacttaaaac ttgttgcagg caatggctca accgtgaacc
    15661 tgaaatttga aacagaatct tttaccgctt atgcagataa aggggctact attaatgcgg
    15721 aaggcaaaag caaatttttg gatatagaag tggcgtcggg cggcattatg aatggttttg
    15781 agtttatttg tgatagtgcc gatgtcaggt gtaatgctgg aggaatggta aaaataaacg
    15841 caaaacgata tttgaaagct gcagcctcga ctggcggaga gattatatac cgtggcaagc
    15901 cggtactaaa ccagaggaca accttgggag gcaaaataga acagatgaag gaatagtatt
    15961 tcttctttct tccctttttt tcttgataaa aaagggaact aaaaaatcaa gatgattgaa
    16021 cgctatacgg tttttcattg attggccttc tcgttgcagg aacgctctcg cctctggcga
    16081 gagcacgttc taaagaaggc ttgataaata atatattcag gaacaccagg catgtcaata
    16141 attgcaataa atatttcttt taagagtaca aagtgcttaa agaaagtagt gtcccagcgc
    16201 ttcagcaata gataaatctg tttggctggt aatttttata ttttctttat ttccttctac
    16261 caaaaatatt tctttcccta atttttcaac tacggaagca tcatcagtaa attcagggac
    16321 atactctgta ttgtaggcat ttttaagcag aaggacatcg aaaacttgag gggtttgcac
    16381 ctgcataaaa ttttccctgt taacagggac actcccattt tcagtcattt gcctgataga
    16441 atcaacaaca gggataaccg gaacagcatt tcccttaatt tttgcggttt gaaaacatcg
    16501 ctcaatcaca gcagtactta ccaggggcct tacagcatca tgaactgcaa ccagcccggc
    16561 ttctttgatt tgatttaggc catttttgac agaatgaaaa cgggtttgtc ctccttcgac
    16621 aaagatatgg ggcactgtaa agttgtattg tttgcaaagt gactgccaca actgaatata
    16681 gtcggggggc aaggtaataa tgatttgcat ggaagggtcg taagctaaaa aagcctcaat
    16741 ggtgcgcatt aagataggtt ttccgtgcaa aagaaggaac tgcttaggta ttgcagagcc
    16801 aaatctttgg cctgttcctc cggcaacaat gataacaaaa agcttcatcc gtaaataatt
    16861 tttaaataag gttagatagt ttgttccgca aaagcgtgag aaatcgtttt gacaaattgg
    16921 gctttatcct tgtttgtgtt taacgaatca tgctcgtttc atttgttttt ttagcaatga
    16981 tatttaaaat ctctaaaaat aattactttt acaaagggta aataaagatt tatacaaatt
    17041 atggcgttaa ttagccacaa aaataatttg ttgtaagttg tattcattat ttttcttaat
    17101 tcatcctgtc agtaaaggtt aattttgttg ggataaaata agttttatct ttgcaaattt
    17161 taatttactg atattcaatt gtttaaattt tgacaattgt catttaacgg atggaaaccc
    17221 atgttggcta ttttattact atcgtgtatg cgtttgcaag aacatgggtg tttcgtgtta
    17281 taaattttgc aaaatccaac tctctccaaa aaatatttta gcatgtttgt tatgcggaaa
    17341 tattttttaa ctttgcagcc agaagaagtg tgtaaaaaaa ttggacaagc aaattaaaaa
    17401 taaattgaca atcagcaagt taaataatat attaggaaat acccctcata gcaggggtat
    17461 ttttttgcaa ttttgaataa aactaacagg catggataaa acaataaaaa aagtagttct
    17521 tcttggttca ggagcgttaa aaattggtga agctggcgaa tttgactatt ctggctcaca
    17581 ggcgttgaaa gcccttaaag aagaaggagt ttatacagta ttaataaacc ctaatattgc
    17641 aaccgttcaa acttcagaag gcatagctga taagatttac ttcttgcctg taacccctta
    17701 ttttgttgag aaagttattg ccaaagagaa accagatggc atactgcttt catttggggg
    17761 ccagacagcg ctaaattgtg gtacagagct atataaaacg ggggttctgg aaaaatacaa
    17821 tgtgcgagta cttggtacac ctgttacagc catcatgaac actgaggatc gcgagctttt
    17881 tgtgaatatt ctgaatgaaa tagatgtaaa gacttgtcgt agcgaagcag ttactactgt
    17941 ggacgatgct ataaaagcag ccgaaaggct tggttttccg gttatcatac gagcagcata
    18001 tactttagga ggccaggggt ccggttttgc ttcaaatgtt gaagagcttc gcaggatggc
    18061 cgagaaagct ttttcgtatg cgcctcaaat acttgtagaa gagtcactta aaggttggaa
    18121 ggaagttgaa tacgaggttg tacgagataa gtatgacaat tgtattactg tttgtaacat
    18181 ggaaaacttt gatccgctgg gtatccatac tggtgagagt attgtagtgg ctccatcaca
    18241 gacgcttagt aatagtgaat atcacaaact ccgtgaaata ggtatccgta ctattcgcca
    18301 cattggtatt gttggcgaat gtaacataca gtatgcatta gatccatatt cggaagatta
    18361 tcgcgtaata gaggtaaatg cgcgtctttc acgttcatca gccttggcat caaaagctac
    18421 cggttacccg ttggcatttg tggcagctaa gcttgctttg ggttatgggc ttcatgagtt
    18481 gaaaaactct gtaacaaaga ccacttctgc attttttgaa ccagcgttag attatatagt
    18541 ttgtaaaatt cctcgttggg acttgaacaa gttcattggc gtatctaagc aaattggttc
    18601 ttcaatgaaa tcggtaggag aagtaatggc tatcggtcgc tcttttgaag aagttatcca
    18661 aaagggtttg cgtatgatag gccaggggat gcacggtttt gttggcaacc gtgagattga
    18721 gattgccgat attgacaaag aacttaccga gccaacagac atgcgtattt ttgttatcga
    18781 aaaagccttt gaaaatggct attctgttga caaaatccat gacctgacca aaattgacaa
    18841 gtggtttctt tataagttga aaaatatttt tgaccataaa gaggcactcg agaagtttga
    18901 tagtattgag aaagttccgg atattatggt tcttgaagcc aagaagcttg ggttctctga
    18961 ttttcagatt gcccgaagcg tttcgaagga cgaaaatgct aagatggagc aggctatgct
    19021 ggatgttcgc aagaaccgta tcaaacgtaa catattgcct tatgtaaagc agatagacac
    19081 tcttgctgca gaatatccgg cacaaactaa ctatctgtac ctcacttacc atgctaatga
    19141 gcatgacatc ccatttttct ctgataaaaa atctgttgta gttcttggtt cgggcgctta
    19201 tcgtattggg agctcagtag aatttgactg gtgctcggta aacgctgtgc ttactgcaca
    19261 taagttgaac taccgctcag taatgattaa ttacaatcct gaaacagtaa gtacagacta
    19321 cgatatgtgc gacaggttgt tttttgacga gctttcgttt gagcgtgtga tggacatcat
    19381 tgaattggaa aaccctaaag gtgtcattct ctcgatggga ggccagatac ctaataacct
    19441 ggctatgcgc ttgtaccatc agaacgttcc ggtgcttggt acatctccaa taagtattga
    19501 cagggccgaa aaccgtcaca aattttcgtc gatgcttgat aagcttggta ttgaccaacc
    19561 tcgttggaaa gagctgacca gcatggatga aattgacgat tttattgata aagttggctt
    19621 ccctgtattg attcgaccct catacgtact ttcgggcgct gctatgaatg tttgttcgaa
    19681 ccgtgaagag ttggaacact ttttgaagct tgctgcaaaa gtttcaaaac aataccctgt
    19741 tgtagtgtct gaatttattg agaatgctaa agaaatagag attgatgccg tggccaagga
    19801 tggcgaaatt atggcttacg ctatttctga gcatatagaa tttgccgggg ttcactcagg
    19861 cgatgccaca ctggtattcc ctgctcaaaa gatatacttc gaaacatcaa gaagaatcaa
    19921 gaaaatcagc cgtgagattg caaaagagct taatatttca ggccctttca acatgcaatt
    19981 cctcgctaaa gacaatgata tcaaggttat tgaatgtaac ctgcgtgctt cacgaagctt
    20041 cccattcgtt tcaaaggtgt tgaaagctaa ctttatcgaa ttagctacca aaatcatgtt
    20101 gggtgctgag gtaagcaagc ttgataagtc atactttgag ctggattata taggtatcaa
    20161 agccagccag ttttccttct cacgtttggc caaagctgac cctgtgcttg gggttgacat
    20221 ggcctcgact ggtgaagttg gctgtattgg tgaagattat tacgaggctc ttctgaaagc
    20281 gatgttatct gttggataca gaatgcctaa gaaaaatatt atgctttcta ccggcgaaat
    20341 gcgttcgaag gttgaattgc tctctggtgt aaaaatgctg gccgaaagag ggtacaatat
    20401 ttttgcaacc aaaggtactg ctgctttctt ggaacaaaac ggcattaaag ttactactgt
    20461 tgcctggccg gatgaagatg ctaaggacaa cgtgatggaa tatatccggg gtaagaaatt
    20521 tgacatggtc atcaacattc ctaaaaacct gtcaaaaact gagctaaaca acgactacga
    20581 aatccgtcgt ggtgctatag acttcaatat tccattgatt accaatgccc gtttggcaag
    20641 cgctttcatt catgccattt gcaaaaagag tatcgacgat attaagatta ccagttggga
    20701 cgagtataaa agctaacatt tagcttttaa gatataatta acgaactctt gaaatccttt
    20761 gtctgtttgc ttttgataag cataagacga aggatttttt gcttttaaaa ataaaacagc
    20821 acaggatttg taataactgc atgtctaatg attgtagttt tgcagaccga aaacaataat
    20881 tgatacatat aatgagaaaa acttttgcat taattgccat tctgactttg gctggacaga
    20941 cttttgctgt ggaaaataaa gccgcgatgg ggacaatctc gggcaaaata gttgatggga
    21001 acgataatac cccgcttgaa tttaccaatg ttgctattta taggcaaagt gactcaagct
    21061 ttgttcaggc agccagttca tcagctgatg gaattttcaa agcagaaaat atccctggtg
    21121 gagtttataa agccaaagta atatttgttg gttataaaac ttctgtaatt gattctatcc
    21181 gggtcactcc atttaaaaat acttttgttg gcgaaataaa attgtttgcc aatacaaaaa
    21241 atattggaga agttgtagtt aaaggagagc gaagtatcat tgaaagcaaa attgaccgaa
    21301 aggtttttta tgtggacaaa aatatagcag cccaaagtgg caatgctact gatgttttgc
    21361 aacaagtacc gtcagtacag gttgatgctg aaggtacagt gtcgttgcgt ggttcagaaa
    21421 acgtaaacat catggttaac gggcggccgg ttatgatgga taaaaccaca tttttgcaac
    21481 aaatgccagc taacaccatt gagaagatag aagtaataac caatccttcg gctaagtttg
    21541 acccggaagg aaccgggggc atcatcaaca ttgttttaaa gcaaggggcc ggaacaggga
    21601 cttcggttgt tatttcggcc aatgccagta ccaatgacaa gtacaatttt tcggccaatg
    21661 taggcgttaa cccaggcaaa tacaacattt atgcatctta tggccttcga gacgatacgc
    21721 gtaaaataaa cggcaggcag tttacaacct acttgcacga caattctact catcaaagta
    21781 ttagcgaaga aaaacggacc aacctctcac acatgggcaa gctggggatg gactacaact
    21841 ttacccggac actgacggca ggtgtaagtg gaagttattc gtcaggcgac agaaatggcg
    21901 acgaacggaa caattatact gaaactgaca ctattgtcac cttgtggtac aggtcttcgg
    21961 atgaagaaga aaccaacact cgcattgagg caaattcata tattacccaa aaattcgata
    22021 acaatggtca tgaggctaag ctcgattata cctttaacaa ggaaaatgaa acagaaagaa
    22081 acaactataa agaccggtat tcgctctctg aagcatttat ctctgatgaa ttgcaggata
    22141 atacctcaaa ttacagacgg cataccatcc aggctgatta cactttgcca ctagacaatg
    22201 gtttgaaaat tgagttaggc acaaaagaaa tattttacat gtctgaaaca gatcaaaaaa
    22261 ctttttacga ctcaaccggg acaaatgccc taattgcaga cccttcacgt accagtttat
    22321 ttcattacaa tgaagatatt tatgccttgt atggtacgct gtcatataat tatgggaatt
    22381 ttggcgttat tgggggcttt aggacggaat atttcaaaag cacctttcat tttgacgaaa
    22441 atgacactta tagcaatcct tactggtcat attatccttc aattcatacc acttataaaa
    22501 tcacagaaaa acatgaggtt tcgctcagtt acagcaagcg tgtaaaccgt ccgcgaggtt
    22561 tcatgatcaa cccatttccc gattattctg accctttgca tttacgaaaa ggtaacccta
    22621 acctgaagtc cgaatatatc aattctttag aaggaggata tacatttaaa atagagaaaa
    22681 ttacggttca acctactgta ttttacaggt ttacagataa tgcgtttacg aaccttgcca
    22741 ttgttgatac attaactaac gttacgactt cgaccttcga aaattcaatg aaaagatcat
    22801 catggggtac tgatttgtct gtcactttgc aacctttcaa gttttggaat attaacatca
    22861 gcgcttcggc ttatcagcag gagctgaatg catcaaacct caatagctca accaaaagcg
    22921 actttggata tagtggtaaa atcatgtcca ataccttttt gcctttaggc tttgcctttc
    22981 aggtcaatgc tttttaccgt tcgccattta taaccccaca aggcaagagc gacccattct
    23041 attccatgaa cctcgggttg cgtaaagatt ttttcaaagg taaacttacc gggaatatta
    23101 tgttcaacga cgtttttgac aatacacatt ttgggatgaa cataaaaaat aagcaacagg
    23161 agggcgaagt gtaccgcaaa tttgactcaa ggacaatttt tgttggacta acatataagt
    23221 ttggcaaaca aagtgtaaat aagaaaaaac agaataacaa ccagaacaac gatgagcaaa
    23281 acggaaatga agaaatgatg tattaagcat tttaattact gttgtccggt aaaaatatca
    23341 ggactaaagt ccgtcagtat gttggagttt gtcaccccag ccttaagttt ggggtgagtc
    23401 aatgttatga agtaattggg ggtaaaaaaa caataaacac atcacccaaa aacctttcag
    23461 gcttaaaaac ctgaaaggtt ttttatttcc ggctgacatg catcagaaaa agctctgcac
    23521 aggctattgc atcgctcagt gcatcatgat ggttgagtga aatgccgtat ctgctgcata
    23581 gagctgccag attgcttttg tatattttat aggtacagta ctttgtataa tcaggtacag
    23641 gcaaaccgta aaaagctaat gtgtatgcca acaccgggaa atcaaacccg aatccattgt
    23701 gggctacaac ggtttgtccg gcaataaact tttctatctt tggccaaacc ttatcaaatg
    23761 ttggagagtg catggtatct ttggcactaa tgccatgaat acctgtaaac ctgtaccaat
    23821 aataatttcc tggcggctgt accagcatat tcaatgtttc tgatacctgc ccatcaacca
    23881 cgcggacaag ccctacttgg catatagaat gcctgtatcc ctgggctgtt tcaaagtcga
    23941 tggcagtaaa ggtgttggtc atactttttt gttgcctcaa aggtaaggtt tttgaaagta
    24001 taaaaaaacg ctttgcattt gggaaaagca cgtttgtcac tgttactata ccttccatgt
    24061 ccccaaaacc tggaaggtat agtaacaaaa cttaactatt ttttacgttt taaaaaatag
    24121 caaaattcca aaagcttatt acttttggtc ataagtgaaa aagctttttt atgaataaac
    24181 ttaatgccct gttaattgct ttgatgcttc tttcggtaag tttttcaagt gcccaacaat
    24241 ataaaccgat aaagatactt cccaatacct ttttcaaggg gggcgaaaag cttactttgc
    24301 aattacggta tggctttatt attgggggca atgtaacact tgaacttacc gaatcgaatg
    24361 atctgctgca cgttaaaggc actgcaaaaa caacaggctt ggccgacaaa ctcttcagtg
    24421 taaaggatgt ttacgaaagc tactttatga atagcaataa tcttccggta tttgccattc
    24481 aaaacgttaa agaaggcaaa aaatacacgt attacaacga agtcaaattt aaccatgcat
    24541 tgaaaaaggt aacttctacc aaaagtggtg accacgatgt acccgaaggc atagtcgata
    24601 tgatgtctgt tttttatatg atacgccgtt tggacctttc ggaagccaag gaaggcgaca
    24661 tatacaagat aaacactttt ttttcagaca agctttttcc ttttgagctg cgttatcgtg
    24721 gcaaagagac tgttgaaacc ccgcttggca agatcaggtg catcaaattt gcaccaatag
    24781 tcgaacctgg ccgggtattc aaatctaaag acgacatgtt tatctggttt actgatgacg
    24841 aaaaccgcct tccggtgctc attgctatgg aaatgctcat tggcagtgtc aactgcgaaa
    24901 tgataaagta cgaaaacgta atgaaagagc ctgtttttaa gaaataagta acagctatta
    24961 aagcaatcct tttacttttt ccaaatcttc aggggtatct atggctaccg tttcaaaagc
    25021 tgtagtctgc acgtaaatgt tgtacccgtt ttctatccag cggttttgtt caaggctttc
    25081 ggccagctcg agcgaagaag gggccaatcg ggttatttct ttcaatgtct caaccttata
    25141 cccatatatc ccaatatgct ttaggaattt atgcgatgaa acccaatgct ctggctcaaa
    25201 atttctcata tatggaatgg tcgatctgct aaaataaagg gctttgttgt ctttgtcgcg
    25261 taccaccttt accacattgg ggttaaataa ttcttcagcg ctgtcaataa ctttagaaag
    25321 ggtcgccaat tgcacgttct catcttcaaa acagcttttt aacaaccgca attgttcagg
    25381 tttgataaaa ggttcatcgc cctgaatatt gataacaacg cttattttct gtgatacttc
    25441 ggcttgtatt ttgcgaatag cttcggcaca tctgtcggta ccgcttttgt gagtagtttg
    25501 ggtcataatt gcctttccgc caaagccttc tacatggtcg aaaatacgtt ggtcatcggt
    25561 ggcaacaaag acaaattcaa gggcttttga ggcttgctca taaactctcc ggatcatcga
    25621 ttttccacca atatcaacta aaggtttacc ggggaagcgg gttgacgcat aacgtgctgg
    25681 tattattccg acagctatca taggttttat ttttttgtca aagctaagat aaatagaatc
    25741 aagaaccaag agtcggggaa agaagtagaa tttttaattg taaatttgca tatctaaatt
    25801 tcactatgat gaaaattgtg accctcattg aaaatttggt gcaacaaact aattatgttg
    25861 cagagcatgg gttgtcgttg tatatcgaaa caggtgggca tcggctgttg tttgatacag
    25921 gtctatctgg caagtttata gataatgcag ttaagatggg cattgacctt gcacaggtag
    25981 atgctgtggt aatatcgcat gggcactacg atcatgctgg gggattgaat gctttctttg
    26041 aaatcaatgc aaaagcaaag gtgtatgctc aaaaatcggc atttttgcct aaatttagca
    26101 gaggcttcaa gtatatcggc aatgagacaa acgctgaagt acttgctaaa cggttgattg
    26161 agttaaatga agtaactgaa atagtcccgg gtgtttatct ttttccacag accaagctct
    26221 ttgacaggac tgacactaat ttcaaggatt tttattttca agaaggcgat acaaagcggc
    26281 ctgacgagtt tgatgatgaa cagtttcttg ttcttaaaaa gaacagtgaa atcaatatac
    26341 tgacagcctg ttcgcatcgg ggtatcacta atgtctgtgc tacggctgta gaatatttta
    26401 agttgcctgt tggcatgatt cttggaggtt ttcacataaa agattgccct gaaagccaat
    26461 atcaatttat gattgagtat ttaaaaagac ttgcgccaaa gtatattggc gtttcgcatt
    26521 gcacaggggt cgataagttt gcccggatga tgaacgattg ctcggcaaag gtgttttata
    26581 atcaaacagg taacgagata aacttaaagt aaatggctga aaatactttt gatatacgtg
    26641 cccgcgaatg ggacaaaaac agggtgcatt ttgaccgttc ggaggctatt gctaagagaa
    26701 taaatgctgt tattcctgat ggcagaaaaa tgaaggcgct ggagtttggt gccgggacag
    26761 gaatcttaag ttttttacta aaagataagt tctctgagat aaccctgatg gacagttcgc
    26821 aggagatgat aaatgtatgt actgaaaaaa ttgaagaata caacactccg catatcaaag
    26881 ccattaaata cgatttagag catagcttat accctgagac ctttgatatt atctacagcc
    26941 aaatggtgtt gcaccacgta aaggattata aaaagatact ttacacgttt agcagcatgt
    27001 taaatgacgg cggttacatt gctttagcag atttatatcc tgaagacggc tcatttcatg
    27061 gcgagggggt agaggtgcat aaaggttttg accctgatga gctgtcgcaa atgctctcag
    27121 agatgggttt taaaccgata cttgaagcca aagtatttga gctaaaaagg gacaatggaa
    27181 ttgtatatcc ggtgtttttg ttggtatcgg tcgcgggtat gaaacgttag agtttgcgag
    27241 gtacgtttgt atccgcagag actaaacccg acataggcgc aaataccatc caaccactta
    27301 acccctaatg ttttatgacc cgttattagt ggctgtgttt atttattcat tatctgccgg
    27361 aggaggaggt ggaggtggtg gagtaaattt aaaatcagtc aatttgcttt cattcatccc
    27421 tttttccaca taattatcag gaaattcagg aatatccgga aataatttgt cttcatcaat
    27481 catggcttta aattttagtt aatactgatt tcaatattat aattattttt taaataaatc
    27541 ttcatcttct catttgcttt attctcatta tatttactaa cagaaaaaat cgtatcgtca
    27601 ttaaaagtta gaatttcact tgcttttttc tttaaatcaa aatatttatc agatgcatta
    27661 tccattgata tttttttatt ttgtatttga taccacaatt tctcaaaatc aatacataaa
    27721 atatctagtt tttgcgaacg ctgatttaac tcttttacat attaaaaaaa aggaatataa
    27781 ggttttataa ctgtcaataa ttgagaaata acaataattg cactccaaac gacctgatac
    27841 ttattccata taatccatga ggcaacgctt gatgaagagg aaaatgcaat aaatatattt
    27901 acatttctat cccacttttg atatttatca actaaaatac ttgataaata tgacttatat
    27961 tttaaatcac aaaataatgc ccaaatttgc tctctcatgt ttttgtgttt ttatcattgc
    28021 ctccaactta caaaaattaa tggttaaaac aagccgttta gctgaatatt gattttatcc
    28081 aatacgtagc tcaggtgttc cgggttatcc acaaagttgt aattatcaac atcaataatc
    28141 aagacctttc caaggttata attttcaatc caggcttcgt agcgttcgtt gaggcgttgg
    28201 agatagtcga gacggataga gctctcaaaa tcgcggccac gcttttgtat ttgctgcaca
    28261 agtaccggga ttgaagcccg aaggtatata agcagctgag gaggggtaat gagtgcagac
    28321 ataatatcga acagcgactt gtaagtttca aaatcgcgtg aagacattaa tcccatggca
    28381 tagaggttag gggcaaagat atgtgcatcc tcgtatatgg tacggtcctg tactacggtt
    28441 tctttctttt tctggagcgt gataagctgt tcaaagcggg ttttcaggaa gtaaacctgc
    28501 aagttgaacg accaacgttg catatcttcg taaaaatctg tcaaataagg gttgtggtca
    28561 atattttcat agtggggctc ccagttataa tgttttgcaa gcaaacctgc cagggtagtc
    28621 tttccagaac ctatatttcc gccaatggct atatgcattt taggtaattt aaaagtttaa
    28681 ccaaatatac agcttttctc tttttcggat aaagaatgtt gataaaagaa tgacgaatgg
    28741 cgaatggcga atgacgaatt ttatgccttc ttttctaatg aaggaagcag ttttaaaata
    28801 gattcaatgt gctttcggtc attagaaaga cgggggattt tattttgtcc accgagctta
    28861 ttgttaattt caaaccattt cataaacgta cccgttggca atatggtcac ttggggtatc
    28921 gtgagtgtaa tgttgttgta tcgtttggcc tggtaatcgc tgttgagttt ttgaagttct
    28981 tcatccaata tttgggctac aatatgaggg tttatatctt ttttgtcgca ttctataagc
    29041 cattggtgac atccattgct tttttcgctc atataaacgg gtgcagcagt atagtcgttg
    29101 atacgaatgt ttagtttggc cgatacttct gcaatagcat ggtcggcatt gtccactatt
    29161 agctcttcgc caaaggcatt gatgtaatgt tttgttctac ctgtaatctt tattttgtaa
    29221 gggtttgttg aggtaaacat cacggtgtcc ccgataatat atcgccaaag gccaccattg
    29281 gtagaaatta ccatggcata atttacgccg gttttgacat cagcaagcgc aatggtgttt
    29341 tttttcccct ggtaataatt ggtcatctct ataaattcat aataaatacc atagtctgtc
    29401 atcaggagca tagatgaatc ttgcaaatcg ttttgaatgg caaagaaacc ttcggaagca
    29461 ttgtaagttt ccatgtaatg catgttttca gaagggataa tatttttata gctgttttga
    29521 taaggcgaga aatttactcc tccatgaata aaaagctcga ggttaggcca tacttccaaa
    29581 agatttcttt ttccggttat ttcaagtact cgtttcatca aaaccatgtt ccatgagggg
    29641 actccggcaa aggctgtaat gttttcttta ataatatggt tagacaactt ttcaagcttc
    29701 tcgttccatt cagacagcaa ggctattttt tttggtggtg tgcgaaaaat attggccagt
    29761 gttggtatat tctgtatcat aatggccgat aaatctccgg ctttgatatt gttgcccagg
    29821 ctgtcaattt tgtggcttcc tcccaatgtt attgtttttc cgcaaaaaag ttttgatttt
    29881 ttattgttat gcaaatacaa tattattgaa tctgttccac cacggtaatg gcatttgtga
    29941 agcgattccc atgtaacggg aatatattta cttttatcgt tagtagttcc tgacgatttg
    30001 gccagccagt ctattttgcc tggccaaagg acgttttctt cttcctggcg aattttattt
    30061 atccatggat aaagctcttc gtaatgctga actggcactt tgctggcaaa agattcagga
    30121 gtttttatcg ctgaaaaatg aaaagttttt ccaaattcag ttttttttgc cctgtcaatc
    30181 agccctttaa aagtcatttc ctgaatttca ttggcatgta aagcacaata ttcgattttt
    30241 ttcagtctcg aaaatgtgat ggctttgaag agtttttcaa atattggcat aaatcgtttt
    30301 tatacagctc ttcaaagata tttaaaatgg taaataaagc aaatgcacaa cttgttaaaa
    30361 ctgcttttat tcggtttttg cttcgacact aactgttttt atggcttttt gaagaatagc
    30421 atctaagctt atgaggtatt ctaaagcttt ttgttcgcca acggcttttt tgaattcgtt
    30481 gtgtacaaag tcttttatgt cggtctgcca atcgaggtac gaaagggtgg taatatgtcc
    30541 ttcctggcgc ctttcaaaga gccggttgac gatatttact ttggacagat atgttagata
    30601 ggctttgatg gagataaaaa gctcttttgt caactggttt tcgatgatat gcatctcttc
    30661 cgggataatc tccatgatgc tttggtaaag cgggctgaat gagttttccc agttgaggta
    30721 gttgatataa aagtttacta ttttgaaaag ttcgcggttt tcgtcctggt caatggtgcg
    30781 aaggaaaata gcctgcaact cgtttctaaa gttttctttt atataattgt cgaaaatacg
    30841 ggtaatttca gtggtgtgta cctgatattc ggcgccaaag ttataaagtg taaactttgg
    30901 gttgtcaaaa ggcctcatgc tggcaatgat acgtttcaat acagcaattt cgtcctgtaa
    30961 aagctggtaa agcgggaaaa gtttccgttt ttcgatatta tccagcaatt tatcggaaaa
    31021 atagccatcg agtatcaatc tatagaattc agcgttgttt tgggtgatac gctcaatatc
    31081 actgcgatag attttcaaaa attcgtggat acgacttttt atctgggctt tctttccgtt
    31141 aacaataatc tgaatatata acgggaagaa cttttcgcgt tgaaaggttt tctctttggc
    31201 tcgcaggtta aggtagtgct taactgtaat tttctttttt tccatcggtt aaaagtcaaa
    31261 tttttctaaa gcacaatatt acaatttttt gcaaattgtt agataaaaac aattaatttt
    31321 ttaataacgt ctaaaaatca acatattgtt ttaaagtttt taaacatctg aaaatatgcc
    31381 taaaactttg aaaacccgat attttcacta aatttgcacc acaattttta aaaggtttac
    31441 tgaataacga ttttgagata tgacacagga agaattgttt aaaaagctta tcagccactg
    31501 taaagaatac gggtttattt tcccttccag cgaaatttat gacggtctta gtgcggttta
    31561 cgattatgga caatatggtt cagaactgaa aaacaacata aagaaatatt ggtgggattc
    31621 catggtattg ctcaacgaca acattgttgg tattgatgct gccattttta tgcacccgcg
    31681 tacatgggag gcttcaggtc atgttggggc attcaatgac ccgctgattg acaataaaga
    31741 ttcgaaaaaa cgttatcgtg ccgatgtact tattgaagaa catatagcta agcttgaagg
    31801 caaaatagac aaggaagttg aaaaagctgc caaaaagttt ggagaggctt ttgataaaaa
    31861 ccaattcatt acaaccaatg gccgtgtcct tgaaatccag aaggaaatag ataccgtgcg
    31921 tgcaagattt gttgccgcgc ttgaaaagcc tgaccttgat gccatgcgac aaattattgt
    31981 tgattgcgaa atagtttgcc ctattagtgg aaccaagaat tggactgatg ttcgtcagtt
    32041 caacctcatg tttgatacca aaataggttc agtaagtgat gaagctaacg ttatttatct
    32101 tcgtcctgag accgctcaag ggatttttgt taacttcctg aacgtacaaa aatcaggccg
    32161 tatgaaagtg ccatttggta tagcccagat agggaaagct ttccgtaacg agattgttgc
    32221 ccgtcagttt attttccgta tgcgcgagtt tgaacaaatg gaaatgcaat tctttgttcg
    32281 tcctggttca gagctcgact ggtttgacaa atggaaagaa aaacgacttc gctggcacct
    32341 ggctatgggc attaaccctg agaaataccg cttccacaaa catgataaat tggcacacta
    32401 cgctaatgca gcttatgata tcgaattcca gttcccattt gggtttaaag aagtagaagg
    32461 aatccattca cgtaccgatt acgacttagg ccgtcatgcc cagttttcag gaaagaaaat
    32521 tacttatttt gaccctgaac tgaatcaaag ttatgttcct tatgtaatag aaacatctat
    32581 aggtgttgac cgtatggtat tgttggcatt ggctaatggg tatgctgaag aaaaggtggt
    32641 taaagaagac ggtacaactg acgaacgtgt tgttcttcga attccacctg cattagcccc
    32701 tgttaagctt gcggttctgc ctttggtaaa gaaagatggt ttggacgaaa aagcgcagca
    32761 gattgttaag gcgcttcgtt ttgagttcaa ctgccagtat gatgagaaag actctattgg
    32821 ccgccgttac cgtcgtcagg atgctattgg aacgccttat tgtattactg ttgaccatca
    32881 aactatgcag gatgataccg tgacaatccg taaccgtgac accatgcagc aggaacgtat
    32941 tcctattgcc gggttacaga agtatatggg agacaaggta agcatccgtt ctatgttaga
    33001 aaaaatctaa attataaacg caaaagtcca aagacgcaaa aaagatgttt gaatctcttt
    33061 agcgtctttg ctttttaaaa acacaggcaa tgaaaaacct aaaagcagct tatccgcatt
    33121 taatagcaat tgctattttt gttttattag caagcattta ttttgcccca catttcaaag
    33181 ggtatgggct gagccagcat gatatgcaaa ctgcggccgg gatggcaaaa gaaacagttg
    33241 acttcaacaa aattgaaaac gctgaatcat tttggaccaa ctctgcattc agcggaatgc
    33301 ctacttatca gactgccatg aacaacaaat caaatatccc gcagctgata gaaagcaata
    33361 tttttgtccg gtttttcgat tactctatag gttttatcat cattgccatg ataagctttt
    33421 atgtgctgat gttgtgtttt ggggcaagtc cctggattag catcattggg gccattgctt
    33481 atggttttgc ttccattaac attctatatc ttggtgcagg acacgtgact aaagtacatg
    33541 ctattgcgct gatgcctggt attattgcag gactggtata tgcatatcgt aaaaaccttt
    33601 atattggggc tgcattggtg gcattgtttg tttgccttca tgtgtcggcc aatcacattc
    33661 aggaaacgta ttacttgctt ttcttgatat ttacaattat agcttttgaa ttttacaggt
    33721 tttataaaga aaagctgctt gtttcgttta taaagataac ggggatagtg gctatagcgg
    33781 caataatagg tttgctacct gccattatcg gggtattgtt gccttatgag catggcaaat
    33841 atactacgcg tggcaaatca gagttgacta tcaacgcttc aaaatttgaa gataaaacca
    33901 atggtctgga caatggctat atcaaacaat acagccttgg ttatggcgaa gtttggtctg
    33961 ttgccgtgcc taatattaaa ggtggtgctt cggggtacat tggtaacgat gaaaagaacc
    34021 ttgacaaggt ttctcctgag tttaaaaact atgtggccag tttttcaagt tattggggcg
    34081 agcaattatt ttcaggaggg gcattttact ttggtgccat tatattctta ttgtttgtat
    34141 taggtgtagt ctttattaaa gacaaactca aatgggcatt cttttctata tctatgcttg
    34201 ctgttgtgct ttcatggaaa ttcggattta ttactgattt gtttatcaac aacttcccgt
    34261 tgttcaataa atttagggat accaagatga tgctggtcat tgcccagctt tcattcccat
    34321 ttctgggaat gttgttcctc aatgagcttt ttagcaataa attagataag aaaaagctta
    34381 attatgcttt gattgggact attggcgtgt ttgtaatttt ctatatcatg ccaaccgtat
    34441 ggtttagctt ttttaacagt caggaaaaga attattttga ccaacaaatg gctgcttatg
    34501 gcaacaatcc ttctgcattg cagcaattgg agcagttcag gattgaaatc cagaatgtgc
    34561 gtgtagatat tttcaaagcc gatgtacttc gctcaatatt gtttatgctt gttgccgggc
    34621 tgtttattta cttgttcaat gccgggaagt tagctaagaa atacttgctt attgttcttg
    34681 gagtattgat tttatttgac atttggtcgg ttgacaaacg ctatctcaat aataaaaagg
    34741 aaaacggtga gtacagaagt tgggttacca atgagtcaaa agcaaagcca tatcctgcaa
    34801 gtgaagcaga taaagccatt ttgcaaatgg agttaaggca aaaccctgct ttatctgaaa
    34861 agattgggcc tgctatagaa aaattgagta ctggcaacga caatgttttt aatcatgatg
    34921 aactggcatt gtttgcttta cgagccgaaa ccaattatcg tgtttttact ttgcaagacc
    34981 ctttctcgaa tgcatcggtt tcgtattacc acaaatccat tggggggtac catggggcaa
    35041 agctgaaacg ctaccaggat ttgattgatt tcaggcttag caaagagtat gaggctattg
    35101 ttaatgcatt caacaccaaa tcggatactg ctatttttaa agtgataaac tctggcattc
    35161 caacgttgag catgcttaac acaaaataca tcatttacaa tcctggtgca gcgccaattg
    35221 taaaccccaa tgctttggga aatgtttggt ttgttaggaa tgtaaaagtg gttgcaaatc
    35281 ctgatgcaga aatattggct ttagatagca ttaatccggc acaaacagct gtagttgaca
    35341 agcgctttga aaactttatt aatacagata ttaaagaaga ttcaacggct aagattgccc
    35401 ttgtttctta taaagcaaat gcattggttt atgaaagcaa cactaacact aatcaaatag
    35461 ccatattctc tgaaatttat tacaaagatg gctggaatgc ttatattgat ggtaatccag
    35521 cggattattt cagggcaaac tatgttttgc gtggcattca ggtccctgcc gggaaacata
    35581 agatagagtt caggtttgaa ccaacaattg ttagaacagc ccgaaatatc tcattagcag
    35641 gttctgtgct tttattcttg tttattggag ggatgctttt cttcaattac aaaaagaaaa
    35701 aggaatagtt cagaaccttt tgtttttgaa aagaaaccaa aagaatttga caccaataac
    35761 caggtgatta aaggtggtta agaaaaatcc gtagtgtttt atcatgattc gtaaacgttc
    35821 agataaacct ctgcggatat tttttttgtt tatgccgcca tccagaaacc gcgtcactac
    35881 aagctttgaa ttatggatgc ttttggcttt tttcagggct tttaacaccc attcgtagtc
    35941 ggcagctatc ttgtatttta ggttgtagct gtcggccagt tcgcgtttga cgtatataga
    36001 ctggtgacac acaagcatac cgttaataaa tgaacgccag gtaagctttt caggggcttt
    36061 aaggcgacga aggccaatct cttttccttc ggcatctact atcatggttt ctccataaac
    36121 tatgtctggc aatggattta ggccttcaaa tatactttta agtgtatttt cgtcatatag
    36181 ctggtctccg gcattcagaa agcatacata atagcctttt gctgctttca atcctttatt
    36241 catggcgtcg tacaaacctt tgtcaggttc gctttgccaa taagtaataa tgtcttcgtt
    36301 ttgacgaagg atttctaacg tactatcttt tgaattgccg tctataacga tatattcaaa
    36361 gtttttccag ttttgtttcc ttatgctttc aatagtacgt ggcagaacct tttcgccatt
    36421 gaatactact gtaataatag aaactatagg gctttcattt aatgttctgg cttcacctgt
    36481 tgtcatgttc gatgttaaag ttcttgagtt ttccaatttt ctattataag tcctggtatt
    36541 cgtccaaaat ggctttcatt gttggttaca aaagtcattt cattttcaat ggcaacgcca
    36601 gcaattaata aatcgatgtc atcaacggat tccccgctaa tgcgaagttt agaatagatt
    36661 tcagacgaga ttttacagga ttcttcaatt aaagcaacta cgaaattttc atcaacaaaa
    36721 tcattgaaca tttccaattg cttatgagca tttttatgaa gtaaaccgct cattatttcg
    36781 taataagtta tcagacttat ctcaataata tcatattcag tcagatattt tttaaagttc
    36841 tttactacca aggggtcacc tttaaaatag tatgagagaa tatcggtatc aattaatgcc
    36901 ctattcattg accctgcgag agtttttact ccgatttaaa atcaaatttt cagtaagtac
    36961 tttgaatgct gattcgtcaa tattattcca acatccagca taggatagta ttttggatgt
    37021 cttttctgta ttctgttcca ggttgtttaa atattcattc aaatcactta gcttatttga
    37081 aggaatcctc caaatcttac gtaaaatctg ttgtcttatt tgagtttcgg gtatcatgtt
    37141 tttttcaaat atacaaaatt atcaggtggc aattaattga attatttgct ttttcaacca
    37201 actttaaatg agagatttat acaagttatt atatttttca gcaactacct cttctgcata
    37261 acagtcaagc actttttttc gtgcattttc agacaattga gaatggtcaa cttcataaat
    37321 agcccatttt ataccgtttg ccaaatcgtc aattgatttg tattcggcca aataaccgtt
    37381 ttgcttatgt tcaaccattt cgggtattcc tcctacattg aaagccacaa ccggtgtgcc
    37441 acaggcaaag gattccataa tggtattggg taaattttcc tgtaatgatg aggtaacaaa
    37501 ccagtgcacc gcgttgtaaa gctggctcat catactttcc tcatgaatac tgccaataac
    37561 gttgcatttg aaaggtacct cacgaagaat ctgctcatca gagcgtccca agattacaat
    37621 ttctaactgg cctgacaatt ccgggtttga ttgaattaaa actttcaaag catccctgaa
    37681 ataggtaaaa cctttgaagt agttttttac attgaccgca ccaaaaagca agtacttttt
    37741 attttctttc aatccaaatg ttttcctggc ttctgttttg tctaaaggtt taaaaacatt
    37801 agtatcaata gggtttggaa tgctaactac attgatgttg gcaaacaaag cactttcacg
    37861 ggctttaccg gcaagccatt gactgcatgt tactacagtg aggtttgcct gggcaaaagc
    37921 tttctttttc ttttgcagaa tgcgataaga aaggtcgtgt gatgatggat atttcagaaa
    37981 tgggcattcg ccgcaggatt gcttaaacct gttacactcg ccactatgat gacagccacc
    38041 tgtaaatggc cacatatcgt gaagcgtcca aacgacaggc tttttttgat taaaaatttc
    38101 cttaatacct gttatagaaa taaatccttc gttgatccaa tggaaatgaa tgatgtcagc
    38161 ttgttttact tcttttcttt tatgaatatc ttctcctaca ttggcaatag aaaacaaaaa
    38221 tcgtacagac tttgattttt ctctgacaaa gaaaaaaaat ctttcgtatg caaaacgata
    38281 aaagttgatc cactttttaa gctttgaatg ggtagtagat accatatttt ctgtaatagc
    38341 ctcatctctg accaacataa tggctgttgt gccattgtag ttaagggctt tgaggagcct
    38401 tcggcatgca atggcagctc cccctttttt tgatgaagta ttgataagta caacgagcat
    38461 tagtttttat tcattggttt gtatctgcat caatccaaga atgctgctaa ttttagatgc
    38521 aattggcaac ttgtaatatt cgctcagctt ttgcatttgc atcaggtatt ggatgttttc
    38581 ttccatttca tttttaatca tcgagcgaaa tcccggtctg agcgaagcat agtagttcat
    38641 ttgcttatag gttacatcaa agaaccgctc aatgactgcg ttgcctttat cagtagctcc
    38701 ggcttcaagg tagcagttgg cgatatacaa cgagcccatg tcatcaggca tttttgaagg
    38761 agggagcaac ttttcacatt tgtcaagcac tgcaatggct gagtcttttt tgccctcatt
    38821 gtttaatgct tcggcagtca tggcaaatac acttctaaac ctgattattg ataaggtttg
    38881 tttatggaaa tggtcaagat aaacttttgg cgattgcata ttgccataat cgaagacatt
    38941 catcagcttg ttgtacatgc tgtctgcgtt gagcgagagc tcattttcac tggtattttt
    39001 aacaggcatg agtttataag caaaaccatc gcgttgcaga tagttgttta accaacatgt
    39061 ccctgaggca taattggcaa ggaagtaaat tgggcgtttc cactggtttt gtgcaatgat
    39121 atccagcata attagttggc ttttgataat ataattttcg gggatggtta ttgtgattga
    39181 acttggggct ttgtcgatgt atgatttctc tatatatcct ccttttgcca ggttttcttt
    39241 atttattggc aaagcaagtc ttttgcaggg aatatattct atcttttcat catagccttt
    39301 gatagtcttt gttctggggt cgtcactggc tacaaacttt atagcatcgc taatactaat
    39361 gctgtcttta atgtaatcca gtacataaac cctgtccagc gttccttcca tgtattgttt
    39421 atgatttagg cttattggaa gaggatcgga taaataagcc tttttcttca tttggtctat
    39481 ataactatcg ccgttgagaa gcattgtatt cacaactttt acatcggtac gtatgccttc
    39541 aacttcctgt aaataccata aagggaaagt atcgttatcg ccataggtga acaatattgc
    39601 atttggtgca caagattgta aataattgta tgcataggcc ctggccaggt atcggccact
    39661 acggttgtgg tcgtcccagt tttgaactgc cataagcact ggtacacaaa agatacaaat
    39721 aataatgatg gttataactt ttgccagatg gtcttttttt gatttcaaaa agttgaaaat
    39781 acccattaca cctaagccaa tccagatggc aaaagcataa aacgaaccta cataagcata
    39841 gtcgcgttcg cgcggctgaa ggggagtttg gttaagataa acaacaattg ccagtccggt
    39901 aagtataaac aacgttacgg taactacaaa gtagcgtctg cttttgtcta ataaaaagaa
    39961 aagtcctata agccctaaaa tgaaaggtaa aaagaaatag ttatttctgg aagggtcttt
    40021 tttaatgata tctggaaggt tgtcttgtct tccggtcaga tgagcatcaa taaaagggat
    40081 accgctaatc caattgccat tcagaatacc gtttccctgc tcatcgtttt gtctgccaac
    40141 aaaattccac aggaaatatc gcatatacat gtgaccaacc tggtagctga aaaagaattt
    40201 gaggttttcg ctgaaggttg gtacaacata ctttttgact ttgccgtttt cagtaacatc
    40261 tactaatttg cctttgatgt tggcccaatt gtggtacgat tgaacgtgtt gtgggtcaga
    40321 actgtacata cgcgggaaaa gtgtagtaaa cctgctatcg taggtaatat catagtcgta
    40381 gccaataatc tcatatttgc catccaatgg tacgtatttt ggctcgccat ctttaacttc
    40441 aacaattggg gcattgtaat attggccttt aaatagcggt ctgtcaccat actgttccct
    40501 gtcgagataa ctaagcagcg caaatacgtt ggaaggattg ccttcgttga taggagggtc
    40561 tgcaacagcc ctaatggtaa tcattgcgaa tgatagatat cctataatta tcatggttat
    40621 tgatagtatc gcaagattaa gccatctttt atgctttttt actgaatata tgataaggta
    40681 aacaatagtt cccagtaata atataatata gaaaataatc ccactattgt atggcagtcc
    40741 aattccattg acaaacaaca gctcaaactt agaagccagg attataaggc cttgaattag
    40801 accatacatt acgaaggcaa ggattattat ggaaatgatg attgctgtca aaaagccttt
    40861 cagtgaatat ggatatttct tgaaataata aacaagtaca atagccggga tggcaagtag
    40921 attaagcaaa tgcacaccta tagataatcc catgaggtaa gcaataagga tgagccattt
    40981 gtcagctcgt ggttggtcag cgttttcctc ccatttaagt accaaccaaa atataactgc
    41041 tgtaaataaa gatgacaagg catatacttc gccttcaact gccgaaaacc aaaagctatc
    41101 agagaatgta taagccaaag ccccaacaaa tccacttcct aaaacaagta tgccatgggt
    41161 tgtatcagat ttcatgcttt tttcagcaac acgccatcct atgtgggtaa tactccaaaa
    41221 tagaaacaag atggtaaagg cactggccaa tgctgacagg gagttaacca tggatgctac
    41281 taaagctaca ttattcccgg caaatagtga aaacatcctg gccaacatca taaacagcgg
    41341 tgctcccgga gggtgcccta cttcaagcct gtatgaagtg gcaataaatt cgccacagtc
    41401 ccatagcgag gctgttggtt ctttggtgat aagatatact gtggcagcaa tggcaaacac
    41461 taaccagcca gaaatgttat tgtacctttt aaagtttttc acatgacaaa ttttagaaat
    41521 ggatactatt ctatcatact taagggcagg ctaaattata ctattttgct caaaatgata
    41581 aggcaatttc tgctataaag tgttaacttt gataaaaaaa gagccatgaa ttataaactt
    41641 catttcaaac ctattactga tgaagattat ttcgagataa aagaaatata tgattattat
    41701 attcaccatt ctacggctac ttttcatatt aattcattga gtattcagga gcttaaagag
    41761 tttatctttg aaaatcatca tctttatccg gcttatatga taaaatgtga tggcactact
    41821 gccggatatt gctatttgac atatttcaaa aaacgtgagg cttatgatag gtctgctgaa
    41881 gttactattt acctgaaacc tgagttttgc aataaagaca ttgggcataa ggctttggaa
    41941 catcttgaaa aggaggcgca taaaagacat ttgaaaaacc ttgtggccct tattaccggg
    42001 aataattatc aaagtataaa attgtttgaa aagtcaggtt acgtgaaagt ggcacatttt
    42061 aaaaacattg gggaaaagat gggagaggtg ttggatgttt tggcctatca aaaagaaata
    42121 taattgcagc tattattatg aagaaagata aagaccgtgt aaatgttgta tattcaacaa
    42181 atccaagctt tcagtacgag tataatgtgg ggcctgaagc aattactctt cctccacagc
    42241 agcaaaacct tagggtaaag aatgaccgta aacagcgggc tggcaaaaca gttactgttg
    42301 ttagtggttt tgttggttca aaagaagatt tggaagcgct tggacgtact ctaaaatcta
    42361 aatgcgctac aggcgggagc gttaaagatg gtgagatact tattcagggc gaaaatgctg
    42421 ataaagttgt gcagatatta ctacaagccg gatataaagt taagaaaagc ggtggttgat
    42481 ttagtaaatc aactttcttg tttcaactgt ttttacaagt atttttacta tttcatctac
    42541 tgtaaatgac atacagttga tggtcaggtc aggagaagtg taatcgttgt ttttcccctg
    42601 gaaaaagtcc ctgaattctg cacgtttttt gtcaatgtca atacaagttt gacgggcatt
    42661 ttctaaagta aggtcgtaac gctggctaat gcggacagtt ctccattcaa gcggggctac
    42721 aaggttgata tgtaatgaat ctgctatatc tcgggtaata gcaatgcccc cacgtccgac
    42781 aataatagca tggccatttt caccaatttc tctaattaca cggcctatgg ttgcacgtat
    42841 ttttttatcg ctttggtaat attggccaaa agaaccaaag aaatcttcta aagcactttt
    42901 tttctcatat tcaaaaacat actttatttt ggaaggatcc atttgcagct ctttggccga
    42961 ttcttccaaa atttctttgc tgtaccattt ccaaaggtgt gtatgccctt gttcatcatg
    43021 ctttgcagaa agctcgttgg ccagtttttc agcaataagt tttgccggac aaccgtattc
    43081 acgcgaaata gtaatgacag gccctggccc ggtatgtctt ttctgtgtct cttcgtacct
    43141 gtgtttaaga taagaaatca gatcgccctg cataattgag ttttttgaaa attcctaatg
    43201 atggttaaag cctatctttg aatttgaaaa cttctaaatc aacgtagtat ttactccatt
    43261 gacttagttt tatttggttt tcaacgttca agatgtataa aattataaat aattgccaac
    43321 taaagcaaca tttaagctac ttttattttg aatacaatct tttttatatc atcaggtaca
    43381 gctgtattca tcactcccgg tttgtgggca tctctgggcc agaaaatagc aaaggtgcca
    43441 ggttttaagt ttaggaaact gccgttgcca ctgagaaaca tgatatcctt ttctccattg
    43501 tacgggtcaa taatatccat ctcgggttga taggcaaaac caattttttc cgatcccttt
    43561 gttatcattt ggatatcggc atatctgata tgggcttccc attttacatc ctgctctggc
    43621 tttgatttgt attcattaat cgaagcaaat acgttggctc cgtcaatttc gtatttgcca
    43681 ttagccattt tgctgaggtc atttttttgg ataaaatcta aaaccttctc tatctcaggc
    43741 attacgtggc tatattttcc taaatggttt aaattatcaa aaatcatagt tgttttattt
    43801 aataatgatt cggtttccta aagtttcggc aaaggctttt ttgagtatat taagctgttg
    43861 catttgttcc tgcaaggcaa taatcctgtc cacatcttct ttttcctggg cttcgagaag
    43921 ttgtagtttt atttgtttaa taaagcttat taccttttta tttttaaatt cagtaataac
    43981 agatctgaca agatccggga gttttgattc ttctgtttcg attaccaaat gagcacctcg
    44041 tgcaaatagt tttttgctaa ggttgtattt tttcgtaagc aaatcagcta caacttggtg
    44101 tacctctttg tcctggtgtc gtagaaaata tgcagtggca tcttccggat ttttcaggca
    44161 attaatttca tattcctcaa aaatctgtcg gtaaatgggg tgttgcagct ccaactcatc
    44221 tttccttatc tcgtctatga tataatggtc aacagttaca atgtgcactt gcttttgctt
    44281 tccttttatt tcggtttgtt caaatagtat atggttccca tagttaagta aaagccttat
    44341 aatctcacgt tcgaggaatt caaattcatt agtttgattt aatggtttgt ccgtacgttt
    44401 ctcttcaata actatcggag aaggcgctgc tatatttcct tctgtttttt tgcgccggac
    44461 acgcatgact tctgtgtaaa gcactgcttc atcggtgtgt aaaatgtgtg cacactcttt
    44521 gatatactcg gagcggacaa tattgttggg aataatggct atactgctta taatgtctgc
    44581 aataagcgtg gcccgtttga tggggtcatt tttggcttca tccaacaaca gttttgtttt
    44641 gaaaagtaca aagtcgcttt cgttttttgc aataaaatca aggacttcgc tggccgagtg
    44701 cttttttgaa aaagagtcgg ggtcttcgcc gtcggggagc aacagcaccc tcacgttcat
    44761 cccttgttcg agaatgaggt cgatacctcg cagggcagct ttgattcctg ccatgtctcc
    44821 atcgtacaac acagtaaggt tgttggtaaa ccttttgatt agcctgattt ggttgatggt
    44881 cagtgacgtt ccggacgagg ctacaacatt ttcaatacct acctggtgca tagagataac
    44941 atcggtgtaa ccctctgtca tgaagcattt gtcttgctgt tctatggctt ttttggcctg
    45001 gaacaagcca tacaaaacgt tacttttgtg gtatatttct gattcagggg aattgagata
    45061 cttagctacg tttttatcag tacgcagggt tcgtccacca aaggctatga cctgtcctga
    45121 gatgctgaga atgggaaata tcacgcgtcc ggcaaatttg tcgtaacggt aattgtcctt
    45181 ttcggctacc agtccggctt ttataagatg ttcaagctga tagcctgccc ttagggcttc
    45241 attgagcaat gcatcgcgtt gggtaggaga gaagccgagt tgaaactttt caatcatgtc
    45301 atcgcggata ccacgctccc tgaagtaagc ttttcctaca gcaatacctt cctgatggtt
    45361 gttcagcgtt tttctgaaaa atttagcggc aaaatcggtt acaaccaaca gtgcttcgcg
    45421 ttcggtccgc tgtgcggctt cttcagggct aagttccttt tctacaatag ggatattgta
    45481 ctttttggcc aggtagcgaa gggcatcata atagctcagg ttctcatgtt ccatgataaa
    45541 a
//
LOCUS       DAOSPF010000026        44104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_906_length_44104_cov_8.282163, whole genome shotgun sequence.
ACCESSION   DAOSPF010000026 DAOSPF010000000
VERSION     DAOSPF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_44104_cov_8.282163"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..1307)
                     /locus_tag="PK252_06985"
     CDS             complement(336..1307)
                     /locus_tag="PK252_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14491.1"
                     /translation="MKTLKCIVLSSALFCFGLQYGAAQSQSDAANLYNGGIALMSTDL
                     DGAIVKIDSCIIVCKQIGDSANDLKNKAGQFYADLHYQKAFKLYNKDKNLQASIATAK
                     KAIAFADEYASDKVKSKTETFMVQLYAKLGSEFFKTKEYAKAVMAFDSALAINPEYIS
                     AIQNKTMAYKVLGDEPNFSKTVAILIEKLKAKNDTAQLSSINNTAIDFYSKLGSKANA
                     ANKLDEAIANLTKAETYGTDKDVCYFFADVYNKQKNYDKAIEYAQKGLALEKGNNDAK
                     AKYYYALGVAQLGKGDKDAACGSFKSAQFGQFATAAKAQRTNNKCAD"
     gene            1608..2180
                     /locus_tag="PK252_06990"
     CDS             1608..2180
                     /locus_tag="PK252_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT14492.1"
                     /translation="MEPIPDQILLLKIKENPENFGLVFDAHYKTIFSYIYRRVADYDL
                     ARDLSADTFMKAFLHISHFNWQGISLLSWLYRIATNETNYYFRKNRTNNLLRDLTFGD
                     AYLLTLTEESITEKQLLEEELKLNQDFLKAQQSIKKLDIKYQEVLSLRYFEKKSVLEI
                     AEILNKKEGTIKSLLSRGLEKLKIEFFSAT"
     gene            2238..2690
                     /locus_tag="PK252_06995"
     CDS             2238..2690
                     /locus_tag="PK252_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14493.1"
                     /translation="MENNFEKQMENLETPKTEFVKHQEILKIGMVNAKKSARIGILFI
                     LAPILILLLAFVKIKLLIGFDFFVKFEKMFTTTGENGLPGYLPFILFVVLPLIAFFIN
                     LLAVSHFYINKTTRELIITIKYRLRNVIVLIVSTIIIATFFVYFIFFY"
     gene            2731..3480
                     /locus_tag="PK252_07000"
     CDS             2731..3480
                     /locus_tag="PK252_07000"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOT14494.1"
                     /translation="MKRLYLFNFLIISIFSANAQQQPAKEIVRKAYDLMQGQSNETVM
                     RITIVRPTWQRTISLKTWAKGSDYSLALVTGPAKEKGQTFLKRKNEIWNWVPNIQRMI
                     KLPPSMMSQGWMGSDFSNDDLLKESSIVEDYNHTLLGNEPFDNKQCYKIKLEPKPDAA
                     VVWGYIIKWISKDQYFQLKSEYYDEDGALVKTETASNIKTMGDRQIPTHFELIPADKP
                     GNKTVVEMISVIFNKPISESFFSQQNMKIIR"
     gene            3572..4786
                     /locus_tag="PK252_07005"
     CDS             3572..4786
                     /locus_tag="PK252_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14495.1"
                     /translation="MLILAWRNLWRNKRRTLITAASVFFAVFFAILLRGFHAGTWSYL
                     IDSVLHSYTGYIQVHTKDFWENKSFDYSMAADNPALEKIKGIKGVKGLIPRIESFSLA
                     SYGDKTKGVITVGIDPMIENNFSALDKKIVRGRYFHEKDTGIILSERLANFLKVKVND
                     SLVLLSQGYQGASANGVFRIIGIVKLPAPEFDNQMVYMPLSAAQEFYSMQSRLTSVIV
                     DIDNPQKMDKITKHIAKSIDTKSFEVMTWEDMLVELYQQYVSDEGGGVIMLVLLYIIV
                     GFGVFGTVLMMISERRHEYAIMIALGMQRSSLLKLVGTELFYICFIGVLFGTAFSLPI
                     ITYFHFHPLQISGNLADVYAAFGMEPLLPVAWRTDYIIQQAINVTGIVAIVLLYPIYS
                     VYKLDLTKAIRR"
     gene            4922..5866
                     /locus_tag="PK252_07010"
     CDS             4922..5866
                     /locus_tag="PK252_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT14496.1"
                     /translation="MINRILLTILAITLQQICIAQPVVVKLWPNGAPQSKAEPNYKEQ
                     LVEQLNKKTWFAVTDPELYIYNLKTDTTPKTAVIICPGDNYQKLLFTTEGEDVAKWFN
                     EHGIAAFVLKYRLPSDSIMEDKSIGPLQDAQEAIRIVRRHAKEWNINPKKIGIMGFSA
                     GGHLAATLSTHYNNVVYPLTDTIRARPDFTILIYPMITVKNDATYQQAAYYLLGTKPD
                     IRQALFFSGETQVNKNVAPTFIVHSVNDKVVFVDNSIAYFNALKKNSIAVEMHIYPTG
                     GHGYGLATLGESEKSWPQACLNWLNYVFLKEKNNDKTK"
     gene            5942..7840
                     /locus_tag="PK252_07015"
     CDS             5942..7840
                     /locus_tag="PK252_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14497.1"
                     /translation="MDKKINILHISDVPKNSEHIQAILTTEGINFSMEQVMSEKELIA
                     KLNNAKYDIISIDYSSKSFNSDKILNIAKIHTPKTPVIVVSDFIGEEKTVEIIKQGAT
                     DLILKSNLPRIVFAINRAIKDLNNTKIEKPEDVSIFQTIIDSIPELIVVKNLNGQYMY
                     INKAAEKAFGKKAPDLIGQDDSTAFQPGEPKINIEQELIEKPTTVNTDQIIKLYSGKV
                     NTFNVTICPMYDEHGNTTGIIAVARDVTERKLLEQSLRNAKEKAEESDRLKSIFLHNI
                     SHEIRTPANAAIGFADFIVNPDISIEKRQHYANIISRSCQQILAIIADVVNTTTLESG
                     KINVNITEVNINDIFRTLHAQFEQQAHNKKLELKYSLGLDDKLSTIKTDKTKLTEILS
                     NLIKNAIKFTHEGHVSFGYTLKGEWLEFFVEDTGIGISEDMHQIIFERFRQGDSSIYQ
                     RFGGTGLGLAITKGYIEMLDGKIWVKSEKNQGSEFFFTLPYNLSKTEKEIQDKASATY
                     LDELKTILVAEDEDLNYIFIKEILYSHKIRLIRAMNGKEAVDISKTEKIDLILMDIKM
                     PVMDGLEATKKIRTFNDKIPIIAQTAFATDADKKNVFASGCNDFISKPFTKELLLSKV
                     QAHLSQSL"
     gene            8034..9248
                     /locus_tag="PK252_07020"
     CDS             8034..9248
                     /locus_tag="PK252_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT14498.1"
                     /translation="MLSLCWKNIWRNKTRSLVIIFAIVFGVIGGVGFSAFYNGMIYQR
                     LDAAIGNEVSNIQIHNPHFLENKEINDTIANMASILPILQNNKQIKAFSYRIKTFSMV
                     NSANSSNGLLLLGIDAEKEKKVTGIYQYVKQGHYLDSCKKNSILIGQKLAEKLKIKLR
                     SKVVVTLQSSSGNIASAAFKVAGIYNTGNTSFDERMAYVKYDDLAEIVGFNPGMAHEI
                     AITVTDNKLTDTVTDKLKTKLAGYSVKSWKEIMPELGLMTDSGQYMLLIYMTIIFLAL
                     SFGIINTMLMAVLDRIREIGMLMAIGMNKLRVFIMITLETILLMFTGSFIGLILSYIL
                     VEILKKTGIDLTAFASGMADMGFSAIVYPFIEPNAYIQIVVLVIITGILSSIIPARKA
                     LRLNPSEAIRTQ"
     gene            9317..10003
                     /locus_tag="PK252_07025"
     CDS             9317..10003
                     /locus_tag="PK252_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT14499.1"
                     /translation="MKIIEITNLTKVYSENGNEVVAVNNVNLDFEQGEFTAVVGPSGS
                     GKTTFLNMVGGIDNPTEGQILVDKVNICSLKSSELIDFRMNNIGFVFQAYNLIPVLTA
                     KENVEFIMFLQNKPKAERAKRSAELLNAVGLSDRMNSRPSKLSGGQQQRVAVARALAS
                     RPKFILADEPTANLDSKSAETLLDIMEKLNREENITFIFSTHDPRVVNKARRVITLVD
                     GQVAKDERNN"
     gene            10128..10574
                     /locus_tag="PK252_07030"
     CDS             10128..10574
                     /locus_tag="PK252_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOT14500.1"
                     /translation="MNNNYYNKDFQPFASNLRKTMTKAEACLWKYVLKAKKLNDYSFR
                     RQRPVGNYIADFICFELRLIIEIDGYSHTLDDVCNKDVVKSNDLKNLGYEVIRFTDAE
                     VLNDISNVIRRLEAYINDFENCPPLPPPTGDSTDKHFKTLIMYNII"
     gene            10605..11861
                     /locus_tag="PK252_07035"
     CDS             10605..11861
                     /locus_tag="PK252_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14501.1"
                     /translation="MKENEPERKKYTINFEQAIIKNQSCPPLAGVRGWILKQIIKLLV
                     LFLYTSPLFSQPDSASFSLSGYVSDVQSAAFESIDKSWNNNNIFLNRLNFKWSANDKL
                     FWTAEMRNRLAYGDNFTNFPGTIKSYDKDNGMIDLSFNVASGKSYLLNANFDRLYVSY
                     QMKKLVITGGRQRINWGQTFAWNPNDIFNAYSFFDIDYIERPGSDAIRFQYYNTETSC
                     FELAVKSDSSNKFTIAGLYRFNTHGFDIQTLAGIYEKNDMVAGIGWSGPIGPVSFRSE
                     ISYFRDMKHFADTSGQFLSSIDFDYVFPNSLTITAEYLYANHVAYSLQNISSINSSSV
                     NAKTLALAKHNAVVQVSYPITPLFSGSLAGMYLPNVNGYYISPSMTYSIAQNIDASLY
                     AQIFGLDNSSVSFHYQQVMFKMKLNF"
     gene            11958..14519
                     /locus_tag="PK252_07040"
     CDS             11958..14519
                     /locus_tag="PK252_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT14502.1"
                     /translation="MKFVKFMTVPFLQKKIIYLASLLLLFSGLKAQYARTDMKPVGIL
                     SKLKSTLTIPSKTLMTLDIDSLNRQDSAENIDNRIGILQYVDVDLIKHGKKITQEDSS
                     QVWYYHFYAPNALALGLRFDYYILPEGASMYVYNPSMTEVLGAFTNINNKPDSTLSVM
                     YLEDNEAIVEYHQPASCKKTALLKVGAISMAYKNLASTLAYNDINCPAWTSWNTIKHA
                     ICRMVFDDGGTSYYCSGSLINNTRNDGTPYFLTANHCISSQASASSLVTYFNYESTSC
                     NSKIVSNNKQTLSGAKLCANSAQTDFSLLLFYEKPDETYKAYLAGWDNTNHKITFATV
                     IHHPKGLYKSIAQSSTGAKMYPFTIDWDDGTLTKANSHWESTFTNGTINSGSSGSPLF
                     DQNVRIVGQLHGGDNTTNYYGALHVSWQLSTEPTSQLKYWLDPDNTGLTSIDGASLGD
                     IPITNFSIVPLNPCIGAEVAMIDSSANFPDKWKWEISPADYTFLYNTNDTDASPRIAF
                     NRDTIYSITLTTSNLFGSSKSTVKVQPRLYNLNIAIENPIDSICGYKLNKYPLKVTPN
                     YQYQYNISPASFIDTLVNANEIKLTTKDEAFYNGNFWATLIVKASHGACSGYDTMTTL
                     VEIPKNDMLRNAQFISLGESGPYSNRCASVETSEPHPDANGCSMTNKWCYADTIVNNS
                     LWYRFTGPSSGNINIILSGKNLRAAVYKADDYPELFQLGKSAIVAAADNGTSDEQTLS
                     FKKLAVSPAKNYFLQIDGYKRVTSTIKINMYSDAIELYPNPASNIVNIAVSVNVKSTV
                     SMKIVDATGKTVLAFNNLAPSTSYSLDVSNISPGFYYLYSIIDGQLYKKQLIIVK"
     gene            complement(14521..14904)
                     /locus_tag="PK252_07045"
     CDS             complement(14521..14904)
                     /locus_tag="PK252_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOT14503.1"
                     /translation="MAIDISSADISKFIIIGDRVLIKPKSAQERTTSGLFLPPGVQEK
                     ENIQAGYIIKTGPGYPIPAVSEIDEPWKDKGEEVRYVPLQAKTGDLAVFLQKNAYEIE
                     FNKEKYFIVPHSAILLLIRDEGLFE"
     gene            complement(14996..15304)
                     /locus_tag="PK252_07050"
     CDS             complement(14996..15304)
                     /locus_tag="PK252_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14504.1"
                     /translation="MEEAILNGTNMIFVTDCAGQGFALEHDDITKVIFKALHGSGVND
                     RLLALNLADKVMYRLGLIKSPLKAHTVVELEQMIRFVLNECGQQRASLQVSLVLDKVN
                     "
     gene            15693..16859
                     /locus_tag="PK252_07055"
     CDS             15693..16859
                     /locus_tag="PK252_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HOT14505.1"
                     /translation="MQQPNTNRIEVVDALRGFAIMSIMLLHHLEHFDFWFFPENLPAW
                     LKVVDGAIWKTMFFLFSGKAYTIFALLFGFTFFIMFNKQQQNGKGFRLRFVWRMFLLL
                     GFGFINSLFYEGDILTFYAVLGVSLVLVCKWNNKAVLITAIILLLQPIEMFRLGYAIF
                     HPEYTLPARMSDPLFGKIGEYLSAPSFWGMLKGNITNGKMAVIYWNWENGRIFQAPAL
                     FMLGMLLGRKGYFSTSDANQKFWKKVLFSSIATYLILGLIAMIMKTQALTPAVSDKTF
                     FLLGLWSNVAFTFFWVSLFYILYHKKAFSWLLQKLRPFGKMSLSNYVIQSLVGSFIYY
                     GYGLGLYQYTGATYCLLIGIVLFVLQLWFCKWWLSRFKQGPLEGLWHKGTWVFS"
     gene            complement(16856..17194)
                     /locus_tag="PK252_07060"
     CDS             complement(16856..17194)
                     /locus_tag="PK252_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14506.1"
                     /translation="MTITILTANLLTGVIGDLLTKYKSHYKPLTYTLIAMAIITVILY
                     PLYAKLEIWLTVLSSKIVRSGKSLGGRYLGLFVSFVVCIFILAYFYARMWYHIDLLGI
                     LIHGKILDYF"
     gene            17434..19011
                     /locus_tag="PK252_07065"
     CDS             17434..19011
                     /locus_tag="PK252_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT14507.1"
                     /translation="MEAIIGKIDTFIRPSYKDNKFILQKARLLVATHIITFIYALVHL
                     IIFLFIGFNAAACALSILIITTLTGLFLFKKTISINVATNSIIFFAFLSFITIIVSTG
                     GIFSPTLSWLLPVSLIGFLLANRTIGLFWSTISIFTILTFFVLNKNDVRFPIILQNQY
                     ISLHNVIAYFGSSVFVLLVVLIYDTISRNRTRELELINEKLTESEASLLESMQALENK
                     DFDLQVMNSELLEKTEELMQQTEEIITQKKEIESQRDEIFEQKKEIERKNQNLTDSII
                     YAKRIQQAMLPNKDLIATNCPEHFILFKPRDIVSGDFYWFKQIKNFTYIAAADCTGHG
                     VPGAFMSMLGISILNEIVTKRDLNPPNEVLDELRKRVKKSLHQTGQDGEAQDGMDIAL
                     CLIDKESNEIQFSGAFNPLYIYRKNAHNSYDLIESKADRMPIGIHPHDNRPFTNRRLN
                     LQKNDTLYIFSDGYTSQFGGQKSEKFKSVRLKEILLDIQDKDMQTQQQILEDTITQWM
                     GKSEQVDDILLIGVRIS"
     gene            19141..19929
                     /locus_tag="PK252_07070"
     CDS             19141..19929
                     /locus_tag="PK252_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT14508.1"
                     /translation="MTKKIILTITSLFVLFTPEKSINTILKASPLQTETVINKIDTAT
                     THSIFNISNLGPYVFPVKGKVISPYGKRGHRFHPGTDIKLQLGDTVRAMFKGIVVRSG
                     RYYGYGLLVILKHPDDIETYYAHLSKCIANVGDTVNPGDVIGLGGRTGRATTTHLHFE
                     IRYHAKAYNAQKLFDFEQQKLIAENYSEETPVKLADSKSDTLAAPINTEKNVHEEKIF
                     HIIKKGDTLFSLAKKYGTTVNNICKLNHLSENTILKIGRKLKIQ"
     gene            20154..21863
                     /locus_tag="PK252_07075"
     CDS             20154..21863
                     /locus_tag="PK252_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT14509.1"
                     /translation="MKKKLVFFALGVSLFVLGGFVDNEFKITKNLDIFFNLYKEVNYF
                     YVDDIDPEKIMQSGIDGMLKSLDPYTTFIPESKMNDFKFQTTGQYGGIGAIIRRSKDG
                     VLISEPYEGCPAAKTGLLAGDVIIEIDGKNVKDNTVSDVSELLKGEPGTNVTVLIKRP
                     FSDELIKKTIRREKITIPNVPYFGMLKDSVGYIKLTNFTTDAGEEVRKAVKQLKAQRA
                     KAYILDLRDNPGGLLNEAVKVANCFMPKKQLVVYTKGRIKEATKEHFTIDEPEDTISP
                     VIVLVNRGSASASEIVTGALQDLDRAVIVGQRTFGKGLVQQTRPLSYNTQLKVTTAKY
                     YIPSGRCIQAVDYSHRNEDGSVGHIPDSLIKEFKTRNGRKVYDGGGIIPDIKVIKEKV
                     SDITYNLYLGNYIFDFATQYCAKNKKIETPEKFTVTDTLYDQFITFLKDKDFDYKTKS
                     EEELKKLIDIAKKEKYYQHAEQEFAMLKEKLSHNKEKDLQTFRNEISGFIIDEIVTRK
                     YFQKGRIIASLQNDIELDSAMMVIKNKKRYYSLLQPSTAIVAEKPKTDKDNEEEKDEE
                     ADE"
     gene            22132..22641
                     /locus_tag="PK252_07080"
     CDS             22132..22641
                     /locus_tag="PK252_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14510.1"
                     /translation="METNTKTQVGSYLTFVLASELYAVHVKNVLNIVELTKITKVPRT
                     PEYMLGIINLRGMVLPVVDARKKFGMPETEITTNTCILVIEVTVLNKNLLVGLMVDGV
                     KEVVEIDASQVKEPPSVGFGNNNRFINGVYNDAAGQFIMILDMEEVFSTEEVVALSNH
                     TENVEQQAE"
     gene            22651..23238
                     /locus_tag="PK252_07085"
     CDS             22651..23238
                     /locus_tag="PK252_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOT14511.1"
                     /translation="MQQLQRVTVFCASSTKSDKAFLQSAYNLGCILALNKIAVVYGGG
                     SVGSMGALANGTLYNKGHITGVIPQFMMELEWGNPLVSEMIVTETLSERKKILLQMAD
                     AVIVLPGGSGTLDELADTMANKKLGLYTKPIILVNQANFFNPFLDFFDKMIEEKFMLP
                     AHRQMFSVVKTEVDVLQAIADAPKWENIAMDFAAF"
     gene            complement(23646..24137)
                     /locus_tag="PK252_07090"
                     /pseudo
     CDS             complement(23646..24137)
                     /locus_tag="PK252_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(24165..25016)
                     /locus_tag="PK252_07095"
     CDS             complement(24165..25016)
                     /locus_tag="PK252_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT14512.1"
                     /translation="MKNIIVPIDFSVDSLKGLEFALMISKYLPVKIQLVYVQRKTDVP
                     GNSDEEYKFADRNFTKLVQEYSSGLGENSQLSYIIKKGKVYQEITSQAQAFADSLIIA
                     STHGASGFEELFIGSNAYRIVCSTNKPVITIRRHPVPQKITKIVMPIDISIESRQKVM
                     YTALFASVFGAEVHVVLVSSSQNKKILSRLNGYVAQVCNYLKTNNVKYVTASVIAKSP
                     IQGLLQYTDENRAEMISIINEDGEGITDLIINGEAQQIISKSPVPVLTIRAKPHTIKE
                     SFSTFGG"
     gene            25488..28688
                     /locus_tag="PK252_07100"
     CDS             25488..28688
                     /locus_tag="PK252_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14513.1"
                     /translation="MMRKIAFLLVFLFSTVVAFSQTRTIKGKVIDESGSGLPGATVQL
                     KGTQKGSVTDVDGNYSIVVPTEGGTLVISYVGYSSQEVTIGQSDEINVNLALDVQNLE
                     QVVVVGYGVQKKSLVTGSISKIESKDITAMPATRVEQALQGKTSGVYIAQSSGTPGGA
                     MSIKIRGNSSDGKNDPIYIVDGIKTGSIDFLAPNDIESIEILKDAASCAIYGAEGGNG
                     VVIISTKKGSSSSSIIEYKYQHGWQNVANTIKMMNASQYVKYQKEAALIENNARHYAK
                     INALDSINGTNWMDEIFNQAPSDEHSILISSGSEKATYLLSASYLDQDGIVGGSKNNY
                     TRYTFRFSGDAKVKKWLSVGSNVAYTQSEKQNLTDNSDFYGGIITSALYFDPTVPVYY
                     KDSAEVNKLYRYNDVIINSLVKKGDQYFHNSTITTGDVANPLAMIENTHNTTTTNKIL
                     ADVHASLFIMDGLKFTSNLGLDYALRVDDVFSPKWYYDDTNLQTNDTLVTIQNTYTKY
                     YKYSFENYLSFDRSFGDHSFNIMAGISYENSKPTFLYVQGNRVPYGQQQFAYVHNVRN
                     NPNPAIDGGIGTRDNYDGLEIQNSYFGRIGYNYKERYMLQANFRRDGSSLFGSDNVYG
                     FFPSFSAGWNIARESFFADNVSLINTLKLRVSWGRNGNKQVLQPFGYTSIMNVETSYA
                     LTDGTFAQGAYPNRPGNPAYKWETSQSFDIGLDLGILKNRITIGIDYFEKRTKDQLGE
                     MALVYDLLGYNAKPYENRGEVKNNGVEFDITYRENEKEFKYSITANASYLKNKVLSYG
                     EDGTKKDGYKMNTGDFITRYEANQPVWYFRGYKAIGIFQDTAEINHYGAVNPTTGNFQ
                     LYQKQAKPGDVKFADVAGTDENGKVVMKPDGKIDDADKVNIGDPNPDFIFGLNFSCEF
                     KGFDFGMFWNGVAGNQIFFAAFRGDRGAYNKPEIYFTERWTGPGTSNKYPRATYNDKN
                     GNLAASTLNISDGDYIRLKSLSLGYTLPNNITSKIGISKLRIYGVATNLLTFTSYKGT
                     DPEIGQMDMTTNNSYGIDRGLYPSPQTFTVGVNVNF"
     gene            28717..30252
                     /locus_tag="PK252_07105"
     CDS             28717..30252
                     /locus_tag="PK252_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14514.1"
                     /translation="MKKTYLYIGTLLFMLVFWASCTKDDLEVTPSQENQASYYQYDEQ
                     IEAASWACYSGLKVIDRWGWTQISWGNFTSDDAHAGGESDNDQPGYHSADYFRCTPND
                     MGGNLGNMWSNTWKGINKCNNLITYIKSVKEKDFHRQAVAEAKVLKALYYFYLARMFG
                     GLPIFDKAPVPSDAITRASQEETYAAIERWLKEAIEAKSIDGSKPALTNIKSERISLD
                     AAKALLGKVLIYEASLFNKKDKYSEAITYLSQVNKELTVKFPDIFESDFNDESIFEVN
                     YTKVLGAGNTYYGNYEIQLYGIRSGDVQINENIGSGWGFNQPSKDLVAAFQSQNDIIR
                     LNATVISSDSAQKLHDKFKPNDVISWVNEISGYWDRKHIPNPTKRGPSWQTFENNNIL
                     LRMADVKLLLAEAYNRTGNDNNARIELNKVRARVNLPEVTSSGEELYAAIKLERRLEL
                     ALEGERYFDLVRWGDAASVLGPIGYNETSGPGIATKGLFPIPQGEINRNSGANNLTQN
                     PGY"
     gene            30403..31272
                     /locus_tag="PK252_07110"
     CDS             30403..31272
                     /locus_tag="PK252_07110"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT14515.1"
                     /translation="MKKVMFTLLACGLAFGMANAKEITIKKAATAPNIDGDIEDFVGD
                     VQAMDQKKGDGPHDNTASFQLTYDDNFVYVAVKVEDPTMDTVQSANVWEKDCVEVFFV
                     LNDTVKDGTTYSNGALGAWQLRKIWGGDMTDQPGKGFKAEEGEIKGGYTQEWKLSWDT
                     LAATGYWGGKQFRFECQNGDNDGSGRNSQNFWNSNADDQWNTILNQGTVKLETAVPSR
                     IVSVSKGKAAFSMTIASNGINFNKAVAEVNVYDITGKLVIKANNTNFVYTTALRNGVY
                     VVKAGNESKKFIK"
     gene            31430..32653
                     /locus_tag="PK252_07115"
     CDS             31430..32653
                     /locus_tag="PK252_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT14516.1"
                     /translation="MGPSVLKITKDVSWIGILDKEIVTFDIVMETKYGTTYNSYFINA
                     EKKTIVETAKEKFSAEYLEKVKAVVNPAEIEYIVLNHTEPDHSGSLKHLLEIAPQATV
                     VGSGQALNYLSEMVGKPFKSLKVKDGDVLDLGNKKLRVIGAPNLHWPDSIYTWLEEDK
                     ILFTCDSFGAHFCHDAMFDDQVGNYDEAFKYYFDVILKPFSKFMLKAIEKIRPLPIEI
                     ICNGHGPILRSTWKEKIDRSEKYAKEYLEKQGSEQKNILIAYVSAYGYTKIMAENIAK
                     GIRENSNYEVDVTDIENILLGDLDEKLTKANAIIVGSPTINQNTLLPVYKLFALINPI
                     RDRGKLAAAFGSYGWSGEAVGLIENHLKNLKLKIFCQGLASKFYPHNGKAEQFIAFGK
                     TFAMELAKEKVVEPD"
     gene            32807..33616
                     /locus_tag="PK252_07120"
     CDS             32807..33616
                     /locus_tag="PK252_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT14517.1"
                     /translation="MERTKVLYISQEITPFLPETEMSVIARTLPQGIQERGREIRTFM
                     PRYGCINERRNQLHEVIRLSGMNLIIDNTDHPLIIKVASIQAARMQVYFIDNEDYFQR
                     KALLKDENGVFFDDNDERMIFFCRGVLETVKKLRWAPDIIHCHGWFTSLIPIFIKKGY
                     ADDPLFANAKVITSLYDNDFSGNLHKNFANKLLFEDIDKNDVKILKEPSYNNLIKFAV
                     DYSDGIIFGSETINNEIVSIVKDSKKPFLEYQPTEAIIDKYAEYYDTIIEK"
     gene            33665..35503
                     /gene="glmS"
                     /locus_tag="PK252_07125"
     CDS             33665..35503
                     /gene="glmS"
                     /locus_tag="PK252_07125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOT14518.1"
                     /translation="MCGIVGYIGAKQAYPILINGLKRLEYRGYDSAGISIYNEKAWVF
                     KCKGKVSDLEAFVNNPEIKGSIGMGHTRWATHGEPNDINAHPHQSMNGKFILIHNGII
                     ENYAPLKKRLEDRGYIFKGQTDTEVLVNLIEYIYQKGEISAEEAVRLALHKVVGAYGL
                     VIFCTDEPDKLIAARKGSPLVIGVGEDEYFLASDATPIVEHTKSVIYLNDNDVAVIGF
                     HEMSLKNLKSETAIKPKIQTIDLDIDEIEKNGFEHFMLKEIFEQPKAIMDTCRGRVSI
                     GEMKIHLGGLYTVMPQLIDAKRIIIIGCGTSWHAGLVGEYLFEDLARIPVEVEYASEF
                     RYRNPVIYKDDIVIAISQSGETADTLAAIQLAKAEGATVLGICNVVGSSIPRNTHAGV
                     YTHAGPEIGVASTKAFTCQVTVLTMMAMLIGKEKGVIPMEKYHSLIAQLAEVPEKIEQ
                     ILNNSSAIEKIAEIYHEATNSLYLGRGYMFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDESMPVVFIATRDKSYEKILSNIQEVKARKGVIIAIVSEGDTVIRSLADHVI
                     EIPYCEEMLMPLLTVIPLQLLSYYIAVKRGCNVDQPRNLAKSVTVE"
     gene            complement(35508..36167)
                     /locus_tag="PK252_07130"
     CDS             complement(35508..36167)
                     /locus_tag="PK252_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOT14519.1"
                     /translation="MMHHVQAFLKAIKENNNREWYLANKDWYNNAKTEFEQVTMNLIS
                     ELGKIDKDIAFLEVSQCTYRIYRDIRFSADKTPYKTHMGAYMVKGGKNSGLAGYYFHV
                     EPGGNMLAGGVYMPMPPVLKKLRKEIYDNIDEFLEIVENKNFKKAFPSLDNDMKLQKA
                     PLGFPKDFEHIDYLKYKSYTVSSSFDDKLLASPKMIEKIIEQFAIMAPFIRFINTALT
                     E"
     gene            complement(36428..36502)
                     /locus_tag="PK252_07135"
     tRNA            complement(36428..36502)
                     /locus_tag="PK252_07135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36467..36469),aa:Arg,seq:cct)
     gene            complement(36621..37382)
                     /locus_tag="PK252_07140"
     CDS             complement(36621..37382)
                     /locus_tag="PK252_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOT14520.1"
                     /translation="MTQESLISIRELNLSVGKQQILKNINLDLPTNKITVIIGPSGCG
                     KTTLLKTLNRLTDLNPDVHVKGNIFINQEDILNTNRDITEIRKQMGLLAQRPFPLPMS
                     IFDNVAFGLRMSGRKRKAILARRVEHYLKQVSLWDEVKDRLNEPASALSIGQQQRLCL
                     ARGLAVDPKIILADEPTSALDPISSQAIEQKFLELKNNYTLVVVTHILRQAKRIADYV
                     VFMYLGEVVEHGTAEEFFNNPKEEKTRMYLAGTFN"
     gene            complement(37379..38233)
                     /gene="pstA"
                     /locus_tag="PK252_07145"
     CDS             complement(37379..38233)
                     /gene="pstA"
                     /locus_tag="PK252_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT14521.1"
                     /translation="MKKRNKIEENVFRVLMFVSTILISIVLLLILYGILHKGLWVLSW
                     EMLTSVPKGGYYFGHEGGIANAIVGSVYLSFGAVILAIIIGLPVALFINIHVVQYQRF
                     VHAVRFLLDLLWGVPSIVYGAFGFMLMIWLGLKSSLLAGIITVALFIIPIIIRGIDEV
                     LKTVPRGLLESSLSLGANRTEVGYKIMVRQCMPGIVTALLLSFGRAIGDAAAVLFTAG
                     FTDRIPTGLMQPTATLPLSIFFQLSSPIEEVKARAYAAAVLLTFIVLIISLLSRYFSH
                     KFKKNSIK"
     gene            complement(38371..39255)
                     /gene="pstC"
                     /locus_tag="PK252_07150"
     CDS             complement(38371..39255)
                     /gene="pstC"
                     /locus_tag="PK252_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT14522.1"
                     /translation="MRLRKVKDNLHQTWMIIAIAIVILLPFIIGAGLIYKSSFILHEY
                     PIKDILFSSEWKPQSSKFGLLPFITSSLFVTFFAIVIVLPISLLSAIHLTQYAKKWVL
                     KVTHPVIDILAGIPSVIFGIWGVIAVVPAVQYIAKLFGYTVSGYSILAGSIVLAIMIL
                     PFVLNMMIEIFQTVPEELTEAALSLGASRWQAIKKVILRKAFPGIISAIGLGISRAFG
                     ETIAVLMVTGNVVKIPEGIFQPGYPLPALLANNYGEMLSIPLYDSALMLSALVLFVIV
                     LLFNLVSRLTIIRFEKKI"
     gene            complement(39269..40270)
                     /locus_tag="PK252_07155"
     CDS             complement(39269..40270)
                     /locus_tag="PK252_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT14523.1"
                     /translation="MINKTLFLWILFVVFIISSCTNNPKYHKKSDLEGSITISGAFAL
                     YPITAKWAEEFMKLYPNVTIDVSAGGAGKGMTDALSGMVDLGMVSRPLSEAEINRGAW
                     YIGVVKDAVVPVFSSSNPYADSIRKRGMTQKQLKAIFIDGRLSDWQRIYNGNTKAHVH
                     LFTRSDACGAGEMFGRYLGMNQEALQGVGVFGDPGMADAVKNEPLSLGYNNIIYVYDI
                     KSRHYNKGIEVVPLDLNENRIIDKEEHFYQNLDSLSAAIREGKFPSPPARELYFVSKG
                     KPENKVVKEFLKWVLTDGQQYVKMAGYVELSEKKRIDELSKVRFVLRKGLSSNDSLK"
     gene            complement(40551..41267)
                     /gene="tsaB"
                     /locus_tag="PK252_07160"
     CDS             complement(40551..41267)
                     /gene="tsaB"
                     /locus_tag="PK252_07160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT14524.1"
                     /translation="MPLILSIETSEKLCSVALADGLQILGSEASTEDKSHASSLTLLI
                     DKLLHNCKISYEQLNAVAVGKGPGSYTGLRIGVSTAKGICYSLKIPLIAVDTFTILVN
                     EFIISEFYSKNKKTFDSAESMICAMIDARRMEVYRRFFDSAFNATSDIEAEVINSTSY
                     DNLLKEKRIYFLGSGADKLPNIIVSDNAFFVNNIRPHARSMCKAALAKFEANEVEDVA
                     YFEPFYLKDFVATISKKKVF"
     gene            complement(41280..42602)
                     /locus_tag="PK252_07165"
     CDS             complement(41280..42602)
                     /locus_tag="PK252_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT14525.1"
                     /translation="MSKKNWILLSIVIFLILIAIVGRKAGLIGKEKPVEVAVEEVKKR
                     TIYELVTANGKIEPETEVKISADVSGEIIEVYVEEGQKVEKGQLMLKIKPNIYISARD
                     RATAVVNSAKAGLENAEARKMQVEALFNKSKLNYERNEKLWEQKAISQHEWETAKTDF
                     ESNKAELLAAEKNVQSSKYNLKSAEASLTEAEENLYKTSIYAPMTGIVTRLNVEKGER
                     VVGTEMMAGTELMRVADLNKMEVKVEVNENDIVRVAVNDTAIIEVDAFLGNKFKGVVS
                     EIANSASSSSSAMSDQVTSFEVKILMLPESYKDLINADRKYPFRPGMTANVDIQTEYR
                     TNVLSVPIEAITSRSDSAMQNNDDKTKKKEEKEIDSKEAVFVLNGQSIVKLKKVKTGI
                     QDNNFIEILAGLSEKDKVVVAPYSAVSRKLKDGDVVKVVDKKMLFSNK"
     gene            42680..43405
                     /locus_tag="PK252_07170"
     CDS             42680..43405
                     /locus_tag="PK252_07170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOT14526.1"
                     /translation="MKKALHIISDIGLVEIIKRRQNRRIIVKSGNHQVCLRVSVPYYV
                     SFDEALGFVHKNIDTIRNKLINLKSHSIIPAVINEGFNYKNAFFSVKIERVLPLSPKK
                     EVELCQNGREYIIRVDEKLDLQNEKMQEKLLFYFEKIIRKEAKQYLPDRLKQLSALHN
                     IPFHSVKINNARTRWGSCSSNNQINLTLKLMLLPAHLIDYVLLHELAHVVHKNHSPKF
                     WAFLSSLTDDILQKRNELKKYNL"
     gene            43556..43777
                     /locus_tag="PK252_07175"
     CDS             43556..43777
                     /locus_tag="PK252_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14527.1"
                     /translation="MKKLFLLSVVAGLVITACGPSAEEREAQRIADSTRQADSIAAVE
                     AAQKAQADSIAAAADSAAKAAEAAKGKKK"
     gene            complement(43832..44041)
                     /locus_tag="PK252_07180"
     CDS             complement(43832..44041)
                     /locus_tag="PK252_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14528.1"
                     /translation="MVTYSKLKTDEVALIKSMLQRGVKQKLIAKMFCVSEMQITRIKR
                     GDNWQEVVAIDQENSTSTEKKIKAN"
BASE COUNT        14539 a         8802 c         7870 g        12893 t
ORIGIN      
        1 aaagaaaaat tccttttcat cgcctttttg ttcgatttcg gccgagaagc ctaacgctcc
       61 agttgtatgc acatcctatc actttatggc ttcgagggtg aaagagttaa ggcgataaga
      121 gtttttgcgc aacttttttc gataaaaaag ttgcataaaa aagctgtgtt tattgctatt
      181 caagtttttc cagtaattgg cagagatatt cttatatcga actcacgtta attaagatat
      241 tgaaaagttc tgcgaaaatt gtcagacaca atataaaaaa aaaccggcct gcaaaagcta
      301 caaaccggtt ttggtgattt atgctaattg tttacttagt cggcacattt gttattggta
      361 cgctgagctt tggcagcagt agcaaactgg ccaaactgtg cgctcttgaa tgagccgcaa
      421 gcagcatctt tatcgccttt acctaactgg gcaactccaa gggcatagta atacttagct
      481 ttggcatcgt tgttgccttt ttcaagggct aaaccttttt gggcatattc aatggctttg
      541 tcataattct tttgtttgtt gtaaacatca gcaaaaaagt aacatacatc cttatctgta
      601 ccataagttt cagctttggt aaggtttgca atagcttcat caagcttgtt ggcagcatta
      661 gcttttgaac ccaacttgct gtaaaaatca atggctgtat tatttatgct gctcaattgg
      721 gctgtatcat ttttagcctt tagcttttca atcaaaatag ctacagtttt tgaaaaatta
      781 ggctcgtcac ccaatacttt ataagccatt gttttgttct gaatagcaga tatatactca
      841 ggattgatgg ccaaagcgct atcaaatgcc attacagctt ttgcatactc tttggttttg
      901 aaaaactcag aaccaagttt ggcatataac tggaccataa aagtttcagt ctttgacttt
      961 accttatcag aagcatattc gtctgcaaaa gcaatagctt ttttggctgt ggcaatagat
     1021 gcctgaaggt ttttatcttt attatagagt ttaaatgctt tttggtaatg taagtcggca
     1081 taaaactgtc ctgctttgtt tttcaaatca ttggcagaat ccccaatctg tttgcaaaca
     1141 atgatacaac tgtctatctt tacaatagca ccatcaaggt cagtggacat aagcgctatc
     1201 cctccattgt ataagttagc agcatccgat tgcgattgag cagccccata ctgtaaacca
     1261 aagcaaaata aggctgaact taatacgata catttcaaag tcttcataat caatagtatt
     1321 tgtgttagtt attaatattt tcaaatttga aaaaccaaat taaatgtttt tatatttaaa
     1381 tatcaacatt tttagattaa ttatgaatta ttcagtcaac tgctgttttg cctttcaaaa
     1441 aagcaatttt tcagtatttt catattattt ataaatttct atttacatta caaaacttaa
     1501 cctggattta atgtaattaa taatcaacaa cttacaagta tccttaatta acatttttta
     1561 tcatattgca acctttttac ttttatgcat tgaatacaga aaaaataatg gaacctattc
     1621 ctgaccagat tttgctttta aagattaaag aaaaccccga aaactttgga ttggtctttg
     1681 atgcacatta caaaactatt ttcagctata tatacagaag ggttgctgat tacgacctgg
     1741 cacgtgactt gtctgccgac acttttatga aagcgttttt acatattagc cattttaact
     1801 ggcagggcat atcgctgcta agttggttgt accgcattgc cactaacgag acaaactatt
     1861 attttagaaa aaacaggacc aacaaccttt tgcgagactt aactttcggc gatgcttact
     1921 tgttaaccct aactgaagaa tctataactg aaaaacaact gctggaagaa gaattaaaac
     1981 tgaaccaaga ctttctgaaa gcccaacaaa gtataaaaaa gcttgacatc aaataccaag
     2041 aagtgctttc tttacggtat tttgaaaaaa aatcagtatt agaaatcgcc gaaatattga
     2101 acaaaaaaga aggtaccata aaatcgctcc tatcgcgtgg actggaaaaa ttgaaaatcg
     2161 aattttttag tgcaacctga tggctaaatt gcattttatt aaatagaaca aggaacaaag
     2221 agtaaagaac aaagaaaatg gaaaataatt ttgagaaaca aatggaaaac ctggaaactc
     2281 caaagacaga atttgtgaaa caccaggaaa tactaaaaat aggaatggtc aatgccaaaa
     2341 aatctgcccg cataggcatc ttgtttatcc tggccccgat attaatcctg ttgttagcct
     2401 ttgtaaaaat caagctcctg attgggttcg atttttttgt caaattcgaa aaaatgttta
     2461 caacaacggg cgaaaacggg ctaccgggct atctgccttt tattcttttc gttgtattgc
     2521 ctttaattgc ttttttcatt aatttactgg cagtttcaca tttttacatc aacaaaacta
     2581 ccagagagct aattattacc atcaaatacc gtcttcgcaa tgtcatagtg ctgattgtca
     2641 gcactattat tattgctaca ttttttgttt attttatctt cttttattga aataaacagt
     2701 tgtactttta ttctttctaa tcctcataga atgaaacgtt tatacctttt caatttcctt
     2761 attatatcca tattttcggc caacgcgcaa cagcaaccag ccaaagaaat tgtccgcaaa
     2821 gcttatgacc tgatgcaagg ccaaagcaat gaaacggtaa tgcgcattac cattgtccgc
     2881 cccacatggc aaaggacaat atcgctcaaa acctgggcca aaggctcgga ttacagcctt
     2941 gccctggtga caggcccggc caaagaaaaa ggccagacat ttctgaaacg caaaaacgaa
     3001 atttggaact gggtccctaa catccagcgt atgataaaac ttcctccatc gatgatgtca
     3061 caaggttgga tgggaagcga cttctccaat gacgacctgc tcaaagagtc ttcaatagta
     3121 gaagattaca accacacctt gctgggcaat gaaccctttg acaataaaca gtgctacaaa
     3181 ataaaattgg aacccaagcc tgatgccgca gtagtatggg gctatatcat caaatggata
     3241 agtaaagacc agtattttca gctaaaatct gaatactacg atgaggacgg tgcattggtt
     3301 aagaccgaaa cggccagtaa cattaaaacc atgggcgacc gccaaatccc aacacacttt
     3361 gagctaatcc cggccgacaa gcccggcaac aagactgttg tagaaatgat aagcgtcatc
     3421 ttcaacaaac ccataagcga aagcttcttc tcgcaacaga atatgaagat aattagataa
     3481 tttgaaaatg agggaattcg agaatgaaaa ccaatttaaa caatacaata ccgagtattt
     3541 tcaaatttgc taattttcaa attttcaaat tatgctaatt cttgcttggc ggaacctttg
     3601 gcgcaacaag cgccgcacgc tgataacagc agcatcggta ttttttgcag tattttttgc
     3661 aatcttgctg agaggcttcc atgctggcac atggagctac ctgatagaca gcgtgcttca
     3721 ctcttacact ggttacattc aggttcatac caaagatttt tgggaaaaca aaagcttcga
     3781 ttacagcatg gctgctgata acccggcttt agaaaaaatc aagggcataa aaggcgtaaa
     3841 agggctaatc ccacgtattg agtcattttc attggcttct tatggcgaca agaccaaagg
     3901 ggttatcacc gtaggcattg accccatgat agaaaacaac ttttcggcct tggacaaaaa
     3961 aattgtccgc ggaagatatt ttcacgaaaa agatacaggc atcatccttt cagaacgctt
     4021 ggccaacttc ctgaaagtaa aagtcaacga ttcgcttgtt ttgctaagcc aaggttatca
     4081 aggggcttca gcaaatggag tttttaggat tataggaatt gttaagcttc cggcccccga
     4141 atttgacaat caaatggtat atatgccact ttcggctgct caagagttct attcaatgca
     4201 aagcaggctc acttctgtta tagtagatat tgacaatcct caaaaaatgg acaaaatcac
     4261 caaacatatt gcgaaatcta ttgacaccaa aagctttgag gtaatgacat gggaggatat
     4321 gctggtagaa ctatatcaac agtacgtcag cgacgaaggc gggggcgtga taatgcttgt
     4381 gcttttgtat atcattgtag gcttcggtgt atttggaact gtgctgatga tgatttccga
     4441 acgccgtcac gaatacgcaa ttatgatagc cctcggcatg caacgaagca gtttattaaa
     4501 actggtaggc accgaattgt tttatatatg ctttataggt gttttatttg gaactgcttt
     4561 tagcttgcct attattacat actttcactt tcatcctttg caaataagcg gcaacctggc
     4621 tgatgtctat gcagcattcg gaatggaacc tttattacct gtagcatgga gaacagatta
     4681 tataatacaa caggccatta atgttacggg cattgtagca atagtcttgc tttatcctat
     4741 atattcagtg tataaattag atttgacaaa agctatacga aggtaatcct ttataaatga
     4801 tataacaaga ccttttcaaa aggataaacg aatgttatcg caatagcaat ttttttcaat
     4861 aaaaagagta actttaataa aatgaagaaa attgactaac tgctaataaa ccacaaaatc
     4921 catgataaac aggatacttt tgacaatatt ggcaattacg cttcaacaaa tatgtatagc
     4981 acaacctgtg gtagtaaagc tttggccaaa tggggccccg caatcaaagg ccgagccaaa
     5041 ctacaaagag cagcttgttg aacaattaaa caaaaaaacc tggtttgcgg taactgaccc
     5101 ggaattatat atttataacc taaaaaccga tacaacgccc aaaactgctg tgattatttg
     5161 tcccggggat aattaccaaa aactactgtt caccaccgaa ggcgaagacg tggccaaatg
     5221 gtttaatgaa catggcattg cagcatttgt gcttaaatat aggttgccat ctgatagtat
     5281 tatggaagac aagtcaattg ggcctttgca agatgcacaa gaggctataa ggatagtgcg
     5341 gcgccatgcc aaagaatgga acattaaccc caaaaaaata ggtataatgg ggttttccgc
     5401 cggaggacat ttagcggcta cgctatcaac gcattacaac aacgtagtat atccattaac
     5461 agacacaatc agagcccgtc ctgattttac cattctcata taccctatga taactgtaaa
     5521 aaacgatgca acataccagc aagcagcata ttatctgctc gggacaaaac ctgatatacg
     5581 tcaggcactc ttcttttcag gagaaacaca ggtaaacaaa aatgtagcac caacatttat
     5641 agtacatagt gtaaatgaca aagttgtttt tgtcgataat tcaatagctt atttcaatgc
     5701 attaaagaaa aactccattg ctgttgagat gcacatttac cctaccggcg gacatgggta
     5761 tggattagca acattagggg aatccgaaaa aagctggccg caagcatgtc tcaactggct
     5821 taattatgta tttttaaaag aaaaaaataa tgacaaaaca aaatagcaaa atccatattc
     5881 ataaaacaag ccatcagtat tttgaataat tataaaacca gcacggttta aaaaagctcc
     5941 aatggataaa aaaataaata tcctgcacat aagtgatgtt ccgaaaaatt ccgaacacat
     6001 acaagctatt ctcactactg aaggcattaa cttttctatg gaacaagtaa tgtccgaaaa
     6061 agagctgatt gcaaaactca ataatgccaa atacgacatt atttcaatag attacagttc
     6121 aaaaagtttt aatagcgata aaatacttaa tattgctaaa attcataccc caaaaacccc
     6181 ggtaattgtt gtatcagatt tcattggaga agaaaaaacc gtggaaatta tcaaacaggg
     6241 tgccaccgac ttaatattga aaagcaattt gccaagaata gtatttgcta tcaaccgtgc
     6301 tataaaggac ctaaataaca ccaaaataga aaagccagaa gatgttagca tttttcaaac
     6361 cattattgat agcatcccgg aattgatagt agttaaaaac ctcaatggcc aatatatgta
     6421 tataaataag gccgcagaga aagcttttgg caaaaaagcc ccagacttga tagggcaaga
     6481 cgacagtact gcttttcaac ctggcgaacc taaaataaat attgaacaag agctgattga
     6541 aaaacccact acagttaata cagaccaaat tattaaactc tatagcggaa aggtaaatac
     6601 tttcaatgtt accatctgtc caatgtacga tgagcatggc aatacaacag gtataatagc
     6661 tgtagcacgc gatgttactg aacgaaaact gttggaacaa agcctcagaa atgccaaaga
     6721 aaaagcagaa gaaagcgaca ggctgaaatc catttttttg cataatatct cgcacgagat
     6781 tcgtactcct gccaatgctg ctattggctt tgctgatttc atcgttaacc ctgatattag
     6841 tattgaaaaa cgccagcatt atgccaacat tatatcgcgg agttgtcagc aaatactggc
     6901 aataatagca gatgtcgtta atacaaccac tcttgaatcg ggtaaaataa atgtcaacat
     6961 cactgaggtc aacattaacg atatattcag aaccttgcat gcacagtttg aacaacaagc
     7021 gcacaacaaa aagttagaat taaaatattc tctgggactg gatgacaaac tttcaacaat
     7081 aaaaaccgac aaaacaaaac ttaccgagat attgagcaat ttgataaaaa acgctattaa
     7141 attcacacat gaaggacatg tttcttttgg atacacgcta aaaggcgaat ggctggagtt
     7201 ttttgtcgaa gacacaggga taggcatttc ggaagatatg caccaaatca tctttgaaag
     7261 atttaggcaa ggagacagct ctatttacca gcggttcgga ggtactggtt taggattggc
     7321 aataaccaaa ggttatatcg agatgttgga tggcaaaata tgggtaaaat cagaaaaaaa
     7381 tcaaggttcg gaatttttct tcacattacc ttacaacctc tcaaaaactg aaaaagaaat
     7441 tcaggataag gcttcagcta catatcttga tgaattaaaa acaatattag ttgccgaaga
     7501 tgaagacttg aactatattt tcataaaaga aatattatac tcccataaaa tacggcttat
     7561 ccgggcaatg aacggcaaag aagctgttga cattagtaaa accgaaaaaa ttgacctcat
     7621 ccttatggat ataaaaatgc ccgtcatgga cggacttgaa gctacaaaaa aaatacggac
     7681 ttttaacgat aaaattccta tcattgccca aacagccttt gctacagacg ctgacaaaaa
     7741 aaacgttttt gccagtggct gcaacgattt cataagtaag ccttttacta aagaattatt
     7801 actttccaaa gttcaggccc atttatctca gtcactatag caaaattata tactaaaaac
     7861 ggctctctta cttaaaaaaa caatgttcat cacctcctgg cgaaatattt ggcgaaacaa
     7921 gcgaaagaca ctaattacaa aatgtaaaaa acttttaaag ggattgataa actattacaa
     7981 atccttagcc catcccctat caactaataa ctaacatcta aagactatca actatgctat
     8041 cactttgctg gaaaaatata tggcgtaaca aaacccgaag cttggtaatc atctttgcta
     8101 tcgtttttgg cgttatcgga ggagttggat tttcagcttt ttacaatggt atgatttacc
     8161 aaaggcttga cgcagctatt ggaaatgaag tgtcaaatat tcaaatccac aacccacact
     8221 ttcttgaaaa taaagaaatc aacgacacca ttgccaatat ggcaagtatc ctccctatat
     8281 tgcagaacaa caaacaaata aaggcgtttt catatcggat aaaaacattc tcgatggtca
     8341 actcggccaa cagtagcaac ggacttttgc tacttggcat tgatgccgaa aaagaaaaaa
     8401 aagtaactgg catttaccag tatgtcaaac aagggcatta cttagacagt tgcaaaaaaa
     8461 acagtatcct tatcggccaa aagctggcag aaaaactcaa gataaaactg cggtcaaaag
     8521 tcgtagttac cttacaaagt tcatcgggaa acattgccag tgcggcattt aaagtagcag
     8581 gaatttacaa cacaggcaat acaagctttg acgaacgtat ggcttatgta aaatatgatg
     8641 acttagccga aattgtaggt ttcaatcccg gaatggcaca cgaaatagct attaccgtaa
     8701 cagacaacaa gctaaccgat acagtaacgg acaaactgaa aacaaaactt gccggataca
     8761 gcgtcaaatc atggaaagaa ataatgcccg aattgggact tatgaccgac tcgggacaat
     8821 acatgctctt aatttatatg accatcattt tcctggcatt gagctttggc atcatcaaca
     8881 ccatgctcat ggcagtactt gaccgtatcc gcgaaatcgg gatgctcatg gccattggca
     8941 tgaacaagct cagggtattt attatgatta cgcttgaaac catattgctc atgtttaccg
     9001 gctcattcat tggcctgata ttgtcgtata ttttggtaga aattctcaaa aaaacaggta
     9061 ttgaccttac agcttttgct tccggcatgg ccgatatggg atttagtgct atcgtttacc
     9121 ctttcattga gccaaatgcc tatattcaga ttgttgtttt agttatcatt actggcattt
     9181 tgtcaagtat catccctgca cgaaaggcgc tacggctaaa cccgtctgaa gcaatcagaa
     9241 ctcaatgacg aattaaaaat tacgaattaa aaaatatttc tttgtgtttt agtgtctttg
     9301 cgttaaaaaa aagataatga aaataataga aattacaaac cttactaagg tttacagcga
     9361 aaatggcaac gaagtggttg ctgtaaacaa tgtcaatctt gactttgaac aaggcgagtt
     9421 taccgcggtg gttgggccat ctggctctgg caaaacaacc ttcctcaata tggtaggggg
     9481 cattgacaac cctaccgaag gtcaaatctt ggtggacaag gtaaatatat gttcactaaa
     9541 aagttctgaa ctgattgact tccgaatgaa caatatcggc tttgtctttc aggcctacaa
     9601 cctcatccct gtgctcacag ccaaagaaaa tgtagaattt atcatgttct tgcaaaacaa
     9661 gccaaaagcg gaacgtgcca aacgtagtgc tgagctgctc aatgccgtgg ggctcagcga
     9721 ccgcatgaac agcaggcctt caaagctctc tggaggccag caacaacgcg ttgcagttgc
     9781 cagggcattg gcttcgcgcc ctaagtttat ccttgccgat gagcctactg ccaacctcga
     9841 ctccaaatcg gccgaaacgc tgctcgacat catggaaaag ctcaaccgcg aagaaaatat
     9901 tacctttatc ttttcaaccc acgaccctcg cgtagtgaac aaagcacgac gtgtgattac
     9961 gctggtagat ggacaggtgg ccaaagacga gagaaacaat tagcaaatgt gagaatgagg
    10021 aaatgaggga atgagggaat ttgaaaattt gaaaatgaaa aagaccaccc aaacgcctgt
    10081 caattaaagc tgtcccccgc tgacgggggt agggggtgga ttgagttatg aataacaact
    10141 attacaataa agactttcaa ccatttgcca gcaaccttcg taaaacaatg acgaaggctg
    10201 aggcttgctt atggaaatat gtcctgaaag cgaaaaaatt aaatgactac agctttcgtc
    10261 gtcaaagacc agttggtaat tatattgctg attttatttg ttttgaatta aggttgatta
    10321 ttgaaataga tggatattcg catactttag atgatgtttg caacaaggat gtagtaaaat
    10381 ccaatgattt gaagaattta ggttatgaag taatccgttt tacagatgca gaagttttaa
    10441 acgacatttc taatgtaatc cgaagactgg aagcctatat caatgacttt gaaaattgtc
    10501 cacccctgcc cccgccaacg ggggacagca cagataaaca tttcaaaaca ctcattatgt
    10561 acaacataat ctaagaataa aatagaaaaa ccgagaatgc tgcaatgaaa gaaaatgaac
    10621 ctgaacgtaa gaaatatact atcaactttg aacaagcaat aattaagaat caaagctgtc
    10681 ccccgctggc gggggtaagg gggtggattc tcaaacaaat tatcaaatta ctagttttgt
    10741 ttctctatac ctcacctctt ttttcccaac ccgactctgc ctctttttct ttatcaggat
    10801 atgtaagtga tgtacaaagt gcggcctttg aaagcattga taaatcatgg aacaacaaca
    10861 acatttttct caaccgctta aattttaaat ggtctgccaa tgataagctt ttttggacag
    10921 ccgaaatgcg taaccgcctt gcctatggcg ataattttac caatttccca ggcacaataa
    10981 aaagctatga caaagacaac ggaatgattg atttgagctt taatgttgct tcgggcaaat
    11041 cgtatttact caatgccaat tttgacaggc tatatgtaag ttatcaaatg aaaaagcttg
    11101 tcatcacagg cggccgtcaa cgcatcaact ggggacaaac ttttgcctgg aacccaaacg
    11161 acattttcaa cgcctattct ttctttgata tagactatat cgaacgcccc ggaagtgatg
    11221 ctatacgctt tcaatactac aataccgaaa cgtcttgttt tgaacttgca gttaaatctg
    11281 acagtagcaa caaatttact attgcaggcc tttacaggtt taatactcat ggcttcgaca
    11341 ttcagacttt agcaggtatt tatgaaaaga acgatatggt tgccggcatt ggctggtcag
    11401 ggccaattgg tccagtttcc tttcgcagcg aaataagcta ttttcgtgac atgaagcatt
    11461 tcgccgatac ctctggccaa tttctttcct ctattgactt tgactatgtt tttcctaatt
    11521 cacttaccat tacagccgaa tacctttatg caaaccacgt tgcatactca ttgcaaaaca
    11581 tttcgtcaat taactcttca tctgtaaatg ccaaaactct ggcattagcc aaacacaatg
    11641 ccgtagtcca ggtttcgtac ccaataaccc ctttattttc aggctctttg gcaggtatgt
    11701 atttgccaaa tgttaatggc tattatattt cacccagcat gacttattct atagcacaaa
    11761 atatagacgc ttcgttatac gctcaaattt ttggcctcga taatagtagt gtttctttcc
    11821 actaccagca ggtcatgttt aaaatgaaac taaactttta agttttttta cgtatatact
    11881 tcatactttt ggaagccaaa attgtttagg gtaaaactgt gttcaggagt ataacctgaa
    11941 tacaacatgc ccgaaatatg aaatttgtaa aattcatgac tgtacctttt ctccagaaaa
    12001 aaatcatata cttagcaagt cttttgcttt tattttctgg tttgaaggcc caatatgccc
    12061 gtaccgacat gaaacccgtc gggatactgt caaagctaaa atctacactt accatccctt
    12121 ctaaaaccct gatgacgctc gatatagatt cattaaatag gcaagactcc gcagaaaata
    12181 ttgacaacag gattggtatt ttgcaatatg ttgatgttga cctaattaaa catggtaaaa
    12241 aaattacaca ggaagacagc agccaggttt ggtattatca cttttacgcc cccaacgcat
    12301 tagctcttgg tttacgcttt gattattata tactgcccga aggagcttca atgtatgttt
    12361 acaacccttc tatgactgag gttttagggg cttttaccaa cattaacaac aagccggaca
    12421 gcaccctctc tgtcatgtac ctcgaagaca acgaagccat tgtcgaatat catcagccag
    12481 cttcgtgcaa aaaaacagca ttattgaagg ttggggctat atcgatggca tacaaaaacc
    12541 tggcctcaac tttagcatat aacgatatta actgccctgc ctggacaagc tggaatacta
    12601 ttaaacacgc catctgtcgt atggtatttg acgatggtgg cacttcatac tattgttccg
    12661 gttcgttaat taacaatacg cgtaacgatg gcacgcctta ctttttaact gccaaccatt
    12721 gtatcagctc ccaagcttct gcctcttcat tagttactta tttcaattac gaatctacat
    12781 catgcaacag caagattgta tccaacaaca agcaaacttt gtcaggcgca aaattatgcg
    12841 ccaattcggc acaaacagac ttttcgttgt tgctgtttta cgaaaaacca gatgaaacat
    12901 acaaagccta cctggcaggc tgggacaaca ccaaccataa aatcactttt gccacagtta
    12961 ttcatcaccc aaaaggcttg tacaagagta ttgctcaaag ctccaccggg gcaaaaatgt
    13021 atcccttcac tatcgactgg gacgatggaa ctttaacaaa agccaacagc cattgggaat
    13081 ctactttcac aaacggaaca ataaacagcg ggtcttcagg ctctccactg tttgaccaga
    13141 atgtccgaat tgtcggacag ttgcacggtg gcgacaacac tacaaactat tacggggccc
    13201 tgcacgtttc gtggcaatta agcactgaac ccaccagcca gctaaaatat tggcttgacc
    13261 ccgacaatac aggccttact tcaattgacg gagccagttt gggcgacatc ccgataacta
    13321 atttcagcat tgttccatta aacccttgta ttggcgctga agtagccatg attgacagct
    13381 ctgcaaattt ccccgacaaa tggaaatggg aaatcagccc tgctgattat acttttctat
    13441 acaacacaaa tgatacagat gcatctccgc gcatagcatt caaccgcgac actatttata
    13501 gcattacact taccacaagc aacctattcg gctcaagcaa aagtactgtt aaagtgcaac
    13561 ccaggcttta caacctcaac attgccattg aaaaccctat tgactccatc tgtggttaca
    13621 agttgaacaa atacccgcta aaggtaacac ccaattatca ataccaatac aacatttctc
    13681 ctgcctcttt tatcgacaca ttagtcaatg ccaatgaaat aaagctgacc accaaagacg
    13741 aagctttcta caacgggaat ttttgggcaa cattgatagt taaagcctct catggagctt
    13801 gtagcggtta cgataccatg actactcttg tcgaaatccc caaaaatgat atgttacgca
    13861 atgcccagtt tatatcattg ggcgaatcag ggccatactc caatagatgt gcctcggtcg
    13921 aaacctcaga gccacatccg gatgccaacg gatgtagcat gaccaacaaa tggtgctacg
    13981 ctgataccat tgtcaataac agtctgtggt acagattcac agggccttcc agcggaaata
    14041 tcaatattat cctttcgggg aaaaatctca gggctgcagt ttacaaagcc gatgattatc
    14101 cagaactctt tcagctaggc aaatcagcta tagtagcagc agcagataac ggcacttctg
    14161 atgaacagac tttaagtttt aaaaaactgg ccgtcagccc ggcaaaaaac tactttttac
    14221 aaattgatgg atataaacgc gttaccagca ccattaaaat aaacatgtac agcgatgcca
    14281 tcgaattgta cccaaaccct gcatcaaata ttgtaaatat tgcagtatct gtaaatgtaa
    14341 aatcaactgt tagcatgaaa atagttgatg ctacaggcaa aaccgtcctg gcatttaaca
    14401 atctggcccc aagtacaagc tactcattag atgtaagcaa cataagcccc gggttttact
    14461 acttgtacag catcattgac ggacaattat acaagaaaca actgataatt gtaaaataac
    14521 ctattcaaaa agcccctcgt ccctgataag caacaatatg gccgaatgcg ggacgataaa
    14581 gtatttttct ttgttaaact caatttcata agcgttcttt tgcagaaaaa cagccaaatc
    14641 tcccgtcttt gcctgtaaag gaacatatcg aacttcttcg cccttgtctt tccaaggctc
    14701 atcaatttcc gaaacagccg gaataggata acccggccct gtcttgataa tataaccagc
    14761 ctggatgttt tctttttcct gtaccccggg aggcaggaac aaacctgatg tagtacgttc
    14821 ctgcgcagac ttaggcttaa ttagtaccct gtcgccaata ataataaact ttgaaatatc
    14881 tgctgaagaa atatctatag ccataaccgt tttttttatc gcacgcaaaa taacaaaaaa
    14941 agcccaaacg tacaaaatta tgcacgtttg ggctatagat atttaacgat aatgcttaat
    15001 tgactttgtc taaaacaagg ctaacctgta agctggcccg ttgttgtcca cactcattca
    15061 ggacaaaacg gatcatttgc tcaagctcaa caactgtatg agcttttaaa gggctcttga
    15121 taagccccaa acggtacatt actttgtcag ccaggttaag ggccaacaga cgatcattaa
    15181 cgcccgaacc atgcagcgcc ttaaaaatta ctttagtaat gtcatcatgt tccaaagcaa
    15241 aaccttgccc cgcacagtca gttacaaaaa tcatgtttgt tccgtttaaa atagcttctt
    15301 ccatgttgtg atgttttaaa tcatacccca atattacaac ccctcattac acatttcaac
    15361 tttagttatt aacaaccaat atgtttctgt caaaaacctt aatcccagta aggaacataa
    15421 ttgttgataa tgtaaattgt acgctaatgc attggacaaa agtatatttt tcaacagtta
    15481 aaatgcaaat ttttaacatg agtgtttatg ctcaatttga cgaactgcta tttgaaatca
    15541 ttctatttaa gggacttgtg tatctcatac atttattcaa ctgccaattt ggggcattca
    15601 tctttaatta acagtatttt aattgatttt attgggctaa aaagaaatgt tttttacaaa
    15661 ttgcatgctt tataaacatt aaacaccaac ttatgcaaca gcccaacacc aaccgtattg
    15721 aagtggtaga tgccctgcgt ggttttgcca tcatgtctat catgctgctt caccaccttg
    15781 aacactttga cttttggttt ttccctgaaa atctgcctgc gtggctcaaa gtggtagatg
    15841 gtgcgatatg gaaaaccatg ttcttcctgt tttcgggcaa ggcttacacc atatttgccc
    15901 tgctgtttgg ctttactttc tttatcatgt ttaacaagca acaacaaaat ggcaaaggct
    15961 tccgtctgcg gtttgtgtgg cggatgtttc tgttgttagg ttttggcttc ataaactcgt
    16021 tgttttacga aggagatatt cttactttct atgcagtatt gggagtttcg ctggtgcttg
    16081 tttgcaaatg gaacaataaa gccgtgttga ttactgcaat tattttattg ttacagccaa
    16141 tagagatgtt ccgattaggc tatgccattt ttcatcccga atatacctta cctgcacgta
    16201 tgtccgatcc attgtttgga aagattggcg aatacctcag cgccccttca ttttggggaa
    16261 tgctaaaggg caatatcact aacggaaaaa tggcagttat ttactggaac tgggaaaatg
    16321 gacgtatatt ccaggctccg gctttattta tgctgggtat gctgcttgga cgcaaaggct
    16381 acttttcaac ttcagatgct aatcagaaat tttggaaaaa agtcctgttt agctcaatcg
    16441 ctacatatct catcctggga ctgatagcca tgataatgaa aactcaggct ttaaccccgg
    16501 ctgtaagcga taaaactttc ttcctgcttg gtttgtggag caatgtggca tttacattct
    16561 tctgggtatc gcttttctat attttatatc ataaaaaagc cttctcgtgg ctattgcaga
    16621 agcttcgtcc ctttggcaag atgagcctca gcaactatgt tatccagtcg cttgtaggtt
    16681 cttttattta ctatggctac gggctgggat tgtatcaata tacaggtgcc acttattgtt
    16741 tacttatagg tatcgttcta tttgtgctcc aactgtggtt ttgcaaatgg tggctatctc
    16801 gcttcaaaca aggcccgctc gaaggtctgt ggcacaaagg cacatgggtt tttagttaaa
    16861 aataatccaa tatctttcca tgtattaata taccaagcaa atcaatatga taccacatgc
    16921 gggcataaaa ataggccagg atgaagatgc aaacaacaaa gctaacgaac aatccaaggt
    16981 aacgccctcc taacgatttg cccgacctta caatcttgga agacaaaaca gtaagccaaa
    17041 tctcgagttt ggcgtataat gggtacaaaa taaccgtaat tattgccatt gcaatcaaag
    17101 tataggtcaa tggcttgtaa tgactcttgt atttggtcaa caagtcccca ataacaccgg
    17161 ttaacaggtt tgccgttaag atggtgattg tcattaccgc aaatttgaaa gcttttcgat
    17221 acaaattatt cattgttctc agttaactga ttaaatttca tacaattata caattttatt
    17281 ttatttattt acaactgaaa cgctgaaact tgccctgtgc ttaatttccc tttttttata
    17341 aaaaataccc aaaaagtatt aggtcatttt ttacttttat aaagatttta tattatcatt
    17401 gtaaaaaagt taggtatttt tttttttact aacatggagg caatcattgg caaaatagac
    17461 acatttattc gtccctcata caaggacaat aaatttattt tacaaaaagc ccgtttgctg
    17521 gttgccactc atatcatcac ttttatttat gcccttgtac accttatcat tttccttttc
    17581 atcgggttta atgcagctgc atgtgcctta agtatcctta ttattaccac tttaacaggg
    17641 ctttttttgt ttaaaaaaac catatctatc aatgtagcaa ccaattccat catttttttt
    17701 gcattcttaa gcttcataac tattattgtt tcaaccgggg gcattttttc gcccaccttg
    17761 tcgtggttac ttccggtttc gctcatcggc tttttgcttg ccaacaggac tataggcctt
    17821 ttctggagta ccatcagcat ttttaccatt ctgaccttct ttgttttaaa caaaaatgat
    17881 gtgaggtttc ctatcatttt gcaaaaccaa tatatcagcc ttcacaacgt tatagcttat
    17941 tttggatcaa gtgtattcgt gcttttggtg gttctcatat atgataccat cagccgtaac
    18001 aggacacgtg agctggagct gattaatgaa aagcttactg aaagtgaagc atcgttgctc
    18061 gaatcaatgc aagcattgga aaataaagat tttgaccttc aggtaatgaa cagcgaactg
    18121 cttgaaaaga cggaagagct gatgcagcaa actgaagaaa tcattacaca aaagaaagaa
    18181 atagaaagcc agcgtgatga aatatttgaa caaaaaaagg aaatagaacg taaaaaccaa
    18241 aaccttaccg acagcattat ttatgctaaa cgcatccaac aagccatgtt gccaaacaaa
    18301 gacctgatag ccaccaactg tcccgagcat tttatccttt tcaaaccccg cgatattgta
    18361 agtggcgact tttactggtt caaacaaata aaaaacttta cttatatagc tgctgccgac
    18421 tgcacaggac acggcgttcc gggggcattc atgagtatgc ttggcatctc tatcctgaac
    18481 gaaattgtca ctaagcgcga cctcaaccct cctaacgaag tgttggatga acttcgcaaa
    18541 agggtaaaaa aatcgttgca ccaaaccggg caagacggtg aagcccagga cggaatggat
    18601 attgcgcttt gcctgattga caaggaaagt aacgagatac aattttcagg ggcgtttaat
    18661 cccttatata tataccgaaa aaatgcgcac aactcgtacg acctcataga atcaaaagct
    18721 gaccgcatgc ccattggcat acacccgcat gacaaccgcc cctttacaaa cagaagactc
    18781 aaccttcaga aaaatgacac tctctacatc ttttctgatg gatatacatc tcaatttggc
    18841 ggacaaaagt ctgaaaaatt taaatctgtg cgattgaaag aaatactcct cgatattcag
    18901 gacaaagaca tgcaaaccca gcaacaaata ctggaggata ccataaccca atggatgggc
    18961 aaatcagaac aggtagatga tatattgctc attggcgtga ggattagtta actggcttaa
    19021 agattgtcag ttccagtaaa tacaagtatt gtagagttat attttaagat tctataacat
    19081 ttttaaggct tttctttcat ctaaaggttc tatttttgtg cccaaccaaa aatttttccc
    19141 gtgacaaaaa aaattatatt aactattacc tctttattcg tgctttttac ccccgaaaaa
    19201 agtataaata ctatattaaa agccagtcca ttacaaactg aaactgtcat taacaaaata
    19261 gataccgcta caacccactc catttttaat atttccaact taggccctta tgttttccct
    19321 gtcaaaggaa aagtaatcag cccctacggc aaacgtggac acaggtttca tccgggtacc
    19381 gacattaaat tacagttggg cgacactgtg cgggctatgt tcaaaggtat tgtagtccgt
    19441 tcaggcagat attacggcta tggcttgctg gttatcctca aacatcctga tgacattgag
    19501 acatactatg cccacctgag caaatgcatt gccaatgttg gcgacacagt caacccggga
    19561 gatgtaattg gcctcggagg aaggacaggc agggcaacta ctactcacct gcatttcgaa
    19621 ataagatacc acgctaaagc atataatgcc cagaaattat ttgactttga gcagcaaaag
    19681 ctcattgctg aaaattattc tgaagaaact ccagtaaaac ttgctgattc aaaaagtgac
    19741 acccttgcag cccctattaa tacagagaaa aatgtacatg aagaaaaaat attccatatt
    19801 atcaaaaaag gagatacttt gttctcgctg gccaaaaaat acggcactac agtaaacaac
    19861 atttgtaagc tgaaccattt gtccgaaaat actatactca agattggaag aaagctgaaa
    19921 atacagtaaa acatattctt caaaaaaaac tgcaaatagc ctgaaaatca caacaattat
    19981 agccaacact ttgcctcttc ccaaaaaaag tatcataaaa atctcccaag taacaaaaaa
    20041 tgagattatt atattttttt gaggttaaaa catgttaagt atagtgtttg gtgaacaatt
    20101 ttttcggaat attgtaaatt gtttgattta gtcacttttt ttaagttttt accatgaaga
    20161 aaaagcttgt tttttttgca ctcggtgtaa gtttatttgt tttgggggga ttcgtggaca
    20221 acgaattcaa aataaccaaa aacctcgata tatttttcaa cctatacaaa gaagtcaatt
    20281 acttttacgt ggacgacatt gaccccgaaa aaattatgca atctggcatt gatggtatgc
    20341 tgaaatctct cgacccatat accacgttca tccctgaatc taaaatgaac gatttcaaat
    20401 ttcaaactac aggacaatac gggggcattg gggccattat tcgacgtagc aaagacggag
    20461 tgctgataag cgaaccctac gaaggttgtc cggcagccaa aacaggcctc ctggcaggcg
    20521 atgttattat agaaattgat ggaaaaaacg tgaaagacaa tactgtttca gatgtcagcg
    20581 aattgctcaa aggcgaacct ggcacaaatg taaccgtctt aattaaaagg ccttttagcg
    20641 atgagctaat taaaaaaacc atccgccgcg aaaaaattac tatccccaat gtcccatatt
    20701 tcggcatgtt aaaagactct gtcggatata taaagcttac caactttact actgatgcag
    20761 gcgaagaagt ccgtaaagct gttaaacagc taaaagctca acgtgcaaaa gcttatatcc
    20821 ttgatttgag agacaacccc ggaggattgc ttaatgaagc cgtcaaagtg gccaactgtt
    20881 ttatgcccaa aaaacaactc gttgtttaca caaaaggccg cataaaagaa gctactaaag
    20941 aacattttac aatagatgaa cccgaagata ctatcagccc ggttattgtg ctggtcaacc
    21001 gaggctctgc ctcagcgtct gaaattgtta caggggctct gcaagacctt gacagggccg
    21061 tcattgtagg ccaacgtacc tttggcaaag gcctcgtgca acaaacaagg cctttaagtt
    21121 acaataccca gcttaaagta accactgcca aatattacat cccaagtggc aggtgcatac
    21181 aggccgttga ctactcgcac cgcaacgaag acggcagtgt cggccatata ccagactcgc
    21241 tcatcaaaga atttaaaacc cgcaatggcc gtaaagttta tgatggcgga ggaataatcc
    21301 ctgacataaa agtaattaaa gaaaaagtca gcgacattac ctacaatctt tacctgggca
    21361 actacatttt tgacttcgcc acacagtact gtgctaaaaa taaaaaaatt gaaacacctg
    21421 aaaaatttac agttacagat accttatatg accagtttat aacattcctt aaagacaaag
    21481 attttgatta taaaactaaa agcgaagaag aattgaaaaa actgattgat attgccaaaa
    21541 aagaaaaata ttaccaacac gccgaacaag aattcgccat gcttaaagaa aagttgtcgc
    21601 acaacaaaga aaaagacctt caaactttcc gcaacgaaat ttcaggtttt atcattgacg
    21661 aaattgttac ccgtaaatat ttccaaaaag ggcggataat agcttctttg caaaacgaca
    21721 tagaacttga ttcggctatg atggttatca aaaacaaaaa acgctattac agcttgttac
    21781 agccttctac tgcaatagta gccgaaaaac caaagacaga taaagataac gaggaagaaa
    21841 aagacgaaga agccgacgaa taatacttaa aaaaattatc aaaaagaatg gctcaaaaac
    21901 gcttattgcc atatactaaa ccactatcaa cataatcaaa tctattagca ggtttagtta
    21961 ctattacagc tcaaatcaat cattagcaag catttcctgc caacgtaaat gattaattgg
    22021 gggttaaaca tttcttaata cttaatttag aataaaaaat tgaccgctaa ttttttagta
    22081 aactaatatt tcctaacttc gtacaagtaa ttatggttaa ttctaaatct tatggaaaca
    22141 aatactaaaa ctcaggttgg ctcttacctc acatttgtgt tggccagtga attatatgca
    22201 gttcacgtaa aaaatgtatt gaacattgta gaattaacaa aaattaccaa agtgccacgc
    22261 actcctgagt atatgttggg catcatcaat cttcgtggca tggtactgcc tgtggtagat
    22321 gcccgaaaaa aatttgggat gcccgaaact gaaataacca caaatacctg tatattagtt
    22381 attgaagtaa ccgttttaaa taaaaacctg cttgttggcc tgatggttga tggtgtaaaa
    22441 gaagtagttg aaattgacgc ttcgcaggta aaagagccgc cttcagtagg ttttggcaac
    22501 aacaaccgct ttatcaatgg cgtttacaac gacgctgccg gccagtttat catgatactt
    22561 gatatggaag aagtattttc aaccgaagaa gtagtagctt tgagcaacca taccgaaaat
    22621 gttgaacaac aagcagaata accataaaac atgcaacaac tacaaagagt tacagttttt
    22681 tgtgcatcaa gtactaaaag cgacaaagct tttcttcaat ctgcctataa cttaggctgc
    22741 attctggcct taaacaaaat agccgtcgtt tatggcggag gttctgtagg cagtatgggg
    22801 gcattagcca acgggacgct atacaacaaa ggccatatca ccggagtgat ccctcaattt
    22861 atgatggaac ttgaatgggg aaaccctctt gtatcagaaa tgatagtaac cgaaactttg
    22921 tccgaacgaa aaaaaatatt gctccaaatg gctgatgctg taattgtatt gcccggaggc
    22981 tcaggaaccc ttgacgaact ggccgatacg atggccaata aaaaacttgg gctttacacc
    23041 aaacccatta tactggttaa tcaggccaac ttcttcaacc cttttttaga tttttttgac
    23101 aaaatgatag aagaaaagtt catgctcccg gcccaccggc aaatgttttc agtagtaaaa
    23161 accgaagtag atgtattaca agccattgcc gatgccccaa aatgggaaaa catagccatg
    23221 gactttgccg cattttaatt gctattttac tttgtcagtt ctctaaacac ttcttcaaga
    23281 ctattttcct gagcttgcat agaaagaacc aacaggcctt tttcagctat cattttagaa
    23341 attggttcac gaacgtctgt gtgactttca ataaaccatt tcccatcaag aaattctatc
    23401 ctctctattc cttcaatctt ctcaaaacag cttttttcta ttgctccgtt taattccaaa
    23461 acattggtac gctttgacat tttcatttct ttgggcctgt tgtctgctac aactttacct
    23521 ttgttgatta ccacaatacg gtcacatatt gcttcaactt cctgcataat atgagttgaa
    23581 agaagtatag ttctatgctt tcccgtttcc tttattaaat tccgtatctc tataatttga
    23641 tttggatcta accccgaagt aggctcatcc aaaattaata cctccggatc atgaatcaac
    23701 gcttgggcca atccaaccct ctgacgataa cccttcgaca aagcagatat ttgtttgttt
    23761 tgctcagctg tcaaccccgt ctttacaatc atttcattaa ccttctgctt tgcatcgtta
    23821 cgggtataaa aaaaccttgc cgcaaaatac aaatactcct taatgtacat attttcgtaa
    23881 agaggattat gctcaggcaa ataaccaatc ttttttttaa taactaaagg cgaatcccca
    23941 atattttcac cgtcaaataa tactcgccct gatgttggat gtagtatccc ggttattatt
    24001 ttcatcaagg tagtctttcc agccccattt ggccccaaaa aacctgttac ttgcgattca
    24061 acaatattaa aactgacctg atctaaagca gtctgtttgc caaacttttt tgtaacacct
    24121 tcaattgata ctaccataca accctatact ttgctaaaca acttttaccc cccaaaagta
    24181 ctgaaacttt ctttaatagt atgaggttta gcccttattg tgagcacagg cacaggcgat
    24241 ttgctgatta tttgttgagc ttcaccattg attatcaaat ctgttatccc ttcaccatcc
    24301 tcgtttatga tagatatcat ctcagccctg ttctcatcag tatattgcaa taaaccctgt
    24361 atagggcttt ttgcaataac cgaagctgta acatatttca cattattcgt cttcagataa
    24421 ttacatacct gtgcaacata accatttaaa cggctaagta tttttttgtt ttgcgaagaa
    24481 gataccagca ctacatgcac ctctgcccca aaaacagaag caaacaaagc cgtgtacatc
    24541 actttttgtc tcgattcaat agagatatct attggcatca ctattttcgt aattttttga
    24601 ggaactggat gcctgcggat agtaattacc ggcttattgg tagaacaaac aatccgatac
    24661 gcattgctcc caataaaaag ctcttcaaat cccgaagccc cgtgtgtgga tgctataatc
    24721 aggctatctg caaaagcctg ggcctgactg gtaatctcct gatatacctt gccttttttt
    24781 atgatataac ttaattgaga gttttctccc aaaccagaac tatactcctg aaccagtttt
    24841 gtaaaattcc tgtctgcaaa tttgtattct tcatcggagt tgccagggac atcagttttt
    24901 cgctgtacat acaccaactg tatttttaca ggcagatatt tagaaatcat taaagcaaac
    24961 tccaaccctt tgagcgaatc taccgaaaaa tcaataggaa ctataatatt tttcatccgt
    25021 aaataatttt tttaataagg tcggatggaa ctcgcatgat gaattttcat ccttgtttgt
    25081 gtttaacgaa tcatgctcgt ttcataactt taaatattta agttttttga agacttattt
    25141 ttgagctaaa ataaacatta atttttgtaa ttgaatcaaa caggcaaaat attcacagcc
    25201 agaaatgcct gaataaaaat ccaaacaata aaaaatatac ggtttttttt gacgaaaatg
    25261 aaattttcaa tgataaaata tgaaaatttt tcttacgaag tgtaaaaata acaattagcc
    25321 gtcagaaaag gataattgtt aatataacca tatcattttt aacatattac aagcctttaa
    25381 aaatctattt taacaagcgc tgaattgcat ttattttttt gttcgtatat ttttggctca
    25441 gaaaatctta aaatttttta acttaactat aactaaacat tcagctaatg atgagaaaaa
    25501 ttgcattttt attggtgttt ctctttagca ctgttgtggc ttttagccaa accaggacta
    25561 ttaaagggaa agtaattgat gagtcagggt caggtttacc tggggctacc gttcaattaa
    25621 aaggtactca aaaaggttct gtaactgatg ttgatggaaa ttattctatt gttgtcccaa
    25681 ccgaaggcgg cactttagta atttcttatg ttggatacag cagccaggaa gttacaattg
    25741 gtcaatcaga cgaaatcaat gttaaccttg cccttgatgt tcaaaatctt gaacaagttg
    25801 tggttgtagg atatggcgta cagaaaaaga gccttgttac tggttctatt tctaaaattg
    25861 aatcaaaaga cattacagct atgcctgcaa cacgtgttga acaagccctg caagggaaaa
    25921 cctcaggcgt ttacattgct caaagctcgg gtacccctgg tggcgctatg tctattaaaa
    25981 tccgtggtaa cagttctgac ggaaaaaatg acccaatcta catcgtagac ggtattaaaa
    26041 ccgggagcat tgattttctt gcccctaatg atattgaatc tattgaaata ttaaaagatg
    26101 ctgcttcttg tgctatttat ggtgccgaag gcggtaatgg cgttgttatt atttcaacta
    26161 aaaaaggctc ttcaagcagt tctattatcg aatacaagta tcaacacggt tggcaaaatg
    26221 tagccaatac catcaaaatg atgaacgctt cacaatatgt caaatatcag aaagaagctg
    26281 ctttaataga aaataatgcc agacattatg ctaaaataaa tgcccttgac tctatcaatg
    26341 gtaccaactg gatggatgaa atatttaacc aggcaccaag cgacgaacac agtattctta
    26401 tttcttcagg ctctgaaaaa gctacttatc ttttaagtgc atcttatctg gaccaggacg
    26461 gtattgttgg tggaagcaaa aacaactaca cccgttatac attccgcttc tctggtgatg
    26521 caaaagtaaa aaaatggctt tctgtaggta gcaatgtagc ttatacccaa tctgaaaaac
    26581 aaaatctaac agacaactcc gacttttatg gtggcataat caccagcgcc ttatattttg
    26641 accctactgt acctgtttat tataaagact ctgccgaagt aaataaactt tacaggtata
    26701 atgatgttat aatcaactca ttggtaaaaa aaggagacca atatttccac aattcaacca
    26761 taaccaccgg cgacgttgct aaccctctgg caatgattga aaatacacat aacaccacta
    26821 ctacaaataa aattttggct gatgtccatg ccagcttatt tatcatggat ggattgaaat
    26881 tcacttcaaa ccttggcctt gattatgctt taagagttga cgatgtattc agcccaaaat
    26941 ggtattacga tgacactaac ctgcaaacta atgatactct ggttacaatt caaaacactt
    27001 ataccaaata ttataaatat tcttttgaaa actatctttc atttgaccgc agttttggcg
    27061 accactcttt taatattatg gcaggtatat cttatgaaaa ttcaaagcct acatttttat
    27121 atgtacaggg aaacagggtc ccatacggac aacaacaatt tgcttacgta cacaatgtca
    27181 gaaacaaccc caaccctgct attgatggag gtatagggac acgtgacaac tatgatggcc
    27241 tcgaaattca aaattcatac tttggacgta ttggttataa ctacaaagaa cgttatatgt
    27301 tacaggctaa cttccgtcgc gatggttcat ctttgttcgg ctcagacaat gtttatggct
    27361 tcttcccttc gttctctgca ggttggaata ttgcccgcga aagtttcttt gctgataatg
    27421 tttctttgat aaacactttg aaactacgtg tcagctgggg ccgaaacgga aacaaacaag
    27481 tccttcaacc cttcggatat acctctatta tgaatgttga aaccagttac gccttaacag
    27541 atgggacatt tgcccagggc gcttatccaa acaggcctgg taacccggca tataaatggg
    27601 aaaccagcca aagttttgat atcggactcg atttaggtat tttaaaaaac cgcattacca
    27661 taggaattga ttactttgaa aaacgcacca aagaccaact tggcgaaatg gctttagtat
    27721 atgacctctt aggctataac gctaaacctt acgaaaatag aggtgaagtt aaaaacaacg
    27781 gcgtggaatt tgacattact taccgcgaaa acgaaaaaga atttaaatat tctatcactg
    27841 ccaatgcatc atatttgaaa aacaaagttc tttcttatgg agaagacggc accaaaaaag
    27901 atggttacaa aatgaatacc ggagatttta taaccaggta tgaagctaac caacctgtat
    27961 ggtactttag aggttacaaa gctattggga tatttcagga cactgccgaa attaaccatt
    28021 atggagctgt aaacccaacc accggaaact tccaacttta tcaaaaacaa gcaaaacccg
    28081 gtgatgttaa atttgcagat gttgccggca ccgatgaaaa cgggaaagta gtaatgaaac
    28141 ctgacggaaa aattgatgat gctgataaag taaatattgg cgacccaaat cctgatttta
    28201 tcttcggcct taacttttca tgcgaattca aaggctttga ttttggtatg ttctggaacg
    28261 gagtggcagg caaccagatt ttcttcgctg ctttccgtgg tgacaggggt gcttacaaca
    28321 aacccgaaat atacttcacc gaaagatgga caggcccggg cacatctaat aaatatccga
    28381 gagctacata taacgacaaa aacggtaact tagccgctag taccctaaat atctcagatg
    28441 gcgattatat tcgcttaaaa agcttatcat taggatatac tctgcctaat aacataacaa
    28501 gcaaaattgg tatcagcaaa ctgcgcatct atggcgttgc taccaacctg cttactttta
    28561 caagctataa aggtacagac cctgaaattg gccaaatgga tatgacaacc aacaattctt
    28621 atggtatcga ccgtggttta tatccttctc ctcaaacctt tactgttggt gttaacgtta
    28681 atttctaaat tcttaaacct tgtaatattt agcaaaatga aaaaaacata tttatacata
    28741 ggaacattac ttttcatgct ggtattttgg gcatcttgca ccaaagacga tcttgaagta
    28801 accccttcac aggaaaacca ggcatcatac taccagtatg acgaacagat tgaagctgcc
    28861 tcatgggctt gttattcggg cctgaaagta atcgaccgtt gggggtggac acaaataagc
    28921 tggggcaact ttacctctga cgatgcccac gcagggggcg aaagtgacaa tgaccagcca
    28981 ggataccatt ctgccgacta tttcaggtgc actcctaatg acatgggtgg caatcttgga
    29041 aatatgtggt caaatacctg gaaaggcatt aacaaatgta acaacctgat tacctacata
    29101 aaatctgtaa aagaaaaaga ttttcatcgt caggctgtag cagaagccaa agtactaaaa
    29161 gcactatatt acttttacct ggcacgtatg ttcggaggac ttccaatttt tgacaaagcc
    29221 ccggtgccat ctgatgctat aacacgcgct tcgcaggaag aaacctatgc tgctatcgaa
    29281 agatggctga aagaagccat tgaggccaag tccattgatg gctctaaacc agcattaacc
    29341 aatattaaaa gcgaacgtat cagtttagat gctgctaaag cccttttagg caaagtattg
    29401 atttatgaag ccagcctttt caacaaaaaa gacaaatatt cagaagcaat cacatacctg
    29461 tctcaagtta acaaagagct gactgtaaaa tttccagaca tttttgaaag tgattttaac
    29521 gatgagtcta tttttgaagt aaactataca aaagtgttag gtgccggcaa tacttactat
    29581 ggcaactatg aaatacaatt gtatggtatt cgttcgggcg atgtacagat taatgaaaat
    29641 ataggctctg gctggggatt taaccaacca tctaaagatc ttgtagcagc ctttcagtca
    29701 caaaatgaca tcatcaggct taatgccact gtcatttcaa gtgatagtgc acaaaaactt
    29761 catgacaaat tcaaacccaa tgatgttata agctgggtaa acgaaatcag cggatattgg
    29821 gacagaaaac acatccccaa tccgacaaaa agaggcccaa gctggcaaac attcgaaaac
    29881 aacaacatcc tgttgcgcat ggcagatgta aaactgttgt tagctgaagc ttataaccgc
    29941 accggaaacg ataacaatgc ccgcattgaa ctaaataaag taagggcaag ggttaacctc
    30001 cctgaagtaa caagctctgg cgaagaatta tatgcagcca taaaactcga aagaagactt
    30061 gagttagcct tagaaggcga acgttatttt gacctggtaa gatggggtga tgctgcaagt
    30121 gttttaggcc ctattggcta caacgaaacc agcggccccg gaatagctac caaaggctta
    30181 ttcccaatac ctcagggcga aatcaaccga aactcggggg ccaataacct gacccaaaac
    30241 ccaggctatt aaaaagcaca aaaatgtact ttggcttata tgtaagtaat gtcaaagtac
    30301 atttttattt tacaaaattt gcattttata ctttttagtt tatctttgta tctatcattc
    30361 tatattaata tgttttattt attaacttaa aacttacaac ttatgaaaaa agtaatgttt
    30421 actcttcttg cttgtggttt ggcatttgga atggcaaatg ctaaagaaat cacaattaaa
    30481 aaagctgcaa cagctcctaa tattgatgga gatattgaag attttgtcgg ggacgttcag
    30541 gctatggatc agaaaaaagg tgatggccct catgacaata cagcttcttt ccaactaact
    30601 tatgatgaca attttgttta cgtagctgtt aaagttgaag atcctacaat ggatacagtt
    30661 caaagtgcaa acgtttggga aaaagactgt gttgaagtat tctttgtatt gaatgacact
    30721 gttaaagatg gtacaactta ttcaaacgga gctcttggtg catggcagtt aagaaaaatc
    30781 tggggcggtg acatgacaga ccaacctggc aaaggcttta aagctgaaga aggagaaatt
    30841 aaaggtggat atactcaaga atggaaactt tcatgggaca cattagctgc tacaggatat
    30901 tggggtggta aacaattccg tttcgaatgc caaaatggcg acaatgacgg ctctggaaga
    30961 aacagccaaa atttctggaa cagcaatgca gatgaccaat ggaatacaat tcttaaccaa
    31021 ggtactgtta aacttgaaac agcagttcct agcagaatcg tatctgtatc taaaggtaaa
    31081 gcagcttttt ctatgactat tgcttctaac ggcatcaatt tcaacaaagc tgttgctgaa
    31141 gttaatgttt atgacatcac tggtaaatta gttatcaaag ctaacaacac taactttgtt
    31201 tacactacag ctctcagaaa cggagtttat gtagttaaag ctggtaacga atctaagaaa
    31261 ttcattaaat aagtataaca cttattaaaa actaaaagcc atccttcggg gtggcttttt
    31321 ttttgaccat tgtttttaaa taattttggt attttattac ctaaatatcg taaaaagctt
    31381 ttacctttgc ataaaagcaa gtatagtcgt tcatcaaaat aatatcaata tgggaccatc
    31441 agtattaaaa attaccaaag atgtatcgtg gattggaatc ctggacaaag aaattgtgac
    31501 atttgacata gtcatggaaa ccaaatatgg caccacgtac aattcatatt ttatcaatgc
    31561 tgaaaagaaa actatagttg aaacagccaa agaaaagttt tcagccgaat atttagaaaa
    31621 agtaaaagct gtagttaacc ctgctgaaat tgaatatatc gttttaaacc acaccgaacc
    31681 agaccattcg gggagcttaa aacatttgtt ggaaatagcg ccacaagcta cagtagtagg
    31741 aagtggacaa gccctcaact atttgtcaga aatggttgga aagccattta aaagcttaaa
    31801 agtaaaagat ggtgacgtgc ttgaccttgg caataaaaaa cttcgtgtta ttggtgcacc
    31861 caacttgcat tggcccgact caatatatac atggcttgaa gaagataaaa tattgtttac
    31921 ctgcgattct tttggggcac atttttgcca tgatgcaatg tttgatgacc aggtaggcaa
    31981 ttacgacgaa gcttttaaat attactttga tgtaatatta aagcccttca gcaagtttat
    32041 gctcaaagcc atagaaaaga taaggccttt gccaattgaa ataatttgta acgggcatgg
    32101 acctatactg cgttctacct ggaaagaaaa aatagacagg tcagaaaaat acgctaaaga
    32161 ataccttgaa aaacagggtt ctgagcagaa aaacatacta atagcatacg tttcggccta
    32221 tggctataca aaaatcatgg cagaaaatat tgccaaaggc atcagagaaa atagcaatta
    32281 cgaggtagat gttactgata ttgagaacat cctgcttggc gatttggacg aaaaactcac
    32341 taaagcaaat gcaatcatag taggctcacc caccatcaat caaaacacac ttttgccggt
    32401 atataaactt tttgcattaa tcaaccctat ccgcgaccgc ggaaaattgg ctgcagcatt
    32461 tggctcgtat ggatggagcg gcgaagccgt tggcctgata gaaaaccatt tgaaaaatct
    32521 gaaactaaaa atcttttgtc agggtttagc ctctaaattt tacccacaca atggcaaagc
    32581 cgaacaattt atcgcttttg ggaaaacatt tgccatggaa ttggcaaaag aaaaagtggt
    32641 tgaaccagac taaaatattt aacccgctaa gaattaaaaa catacaaata caaacataga
    32701 atttaattca acaacattga gacaagtact tcctttaaaa aattttatct aaaaaagcag
    32761 gatatttttt gtacttttgc aacaatttag ataaattata catcaaatgg aacgtaccaa
    32821 ggtactttat atttcgcaag aaataacgcc tttccttccc gagacagaaa tgtctgttat
    32881 tgcccgtact ttgccacagg gaatccagga acgggggaga gaaatacgta cattcatgcc
    32941 acgctatggt tgtataaacg aacgtcgaaa ccagctccac gaagtaatac gcctgtcagg
    33001 tatgaacctg attatagata acacagacca ccccctgata ataaaagtag catctataca
    33061 ggctgcccgt atgcaagttt atttcatcga caatgaagac tattttcaac gaaaagcttt
    33121 gctaaaagat gaaaatggcg tttttttcga tgacaacgac gaacgcatga ttttcttttg
    33181 ccgtggagtt ttagaaacgg taaagaagtt aagatgggcc ccagatatca ttcattgtca
    33241 tggttggttt acttcgctca tccctatttt tatcaaaaaa ggatacgcag atgacccttt
    33301 atttgccaat gctaaagtga taacttcact ctatgacaat gatttttcag gcaatttgca
    33361 taaaaatttt gccaacaaac tattgtttga agatattgat aaaaatgatg tcaaaatttt
    33421 aaaagagcct tcttacaata atctgataaa atttgccgtt gattattcag atggcattat
    33481 ttttggtagt gaaactatca acaatgaaat tgtcagtata gtaaaagata gtaaaaaacc
    33541 ttttttggaa tatcaaccta cagaagctat tatagataaa tatgctgaat attacgacac
    33601 tatcatcgag aaatagtata ataccccatt tttgaaataa actaacccaa gagaaataat
    33661 tgatatgtgc ggaattgttg gatatatagg tgcaaagcaa gcatacccta ttcttataaa
    33721 tggactaaaa cgtttggaat atcgaggata tgattcagcc ggtatttcga tatataatga
    33781 aaaggcctgg gtatttaaat gtaaaggaaa agtcagcgac ctcgaagcct ttgtgaacaa
    33841 ccctgagatt aaagggtcta taggtatggg gcatacccgt tgggcaacac acggcgagcc
    33901 caatgatatc aatgcccacc cacaccagtc gatgaatggc aaatttatcc tcatccacaa
    33961 tggcattatt gaaaattatg ccccattaaa aaaacggtta gaagatagag gttatatttt
    34021 taaagggcaa accgatacag aagttcttgt aaaccttatt gaatatattt accagaaagg
    34081 tgaaattagc gctgaggaag ctgtacgtct tgccttgcac aaggttgtcg gggcatacgg
    34141 gttagtaata ttttgcacag atgagcctga caagcttatt gctgcacgta aaggcagccc
    34201 gcttgttata ggggttggcg aagacgagta ttttctggca tcagatgcaa caccaatagt
    34261 tgaacacaca aaaagtgtta tttacttaaa tgataacgat gttgctgtta ttggatttca
    34321 tgaaatgagc ctgaaaaacc tgaaaagtga gacggcaata aagcctaaaa tacaaaccat
    34381 tgatttagat attgatgaaa tcgaaaaaaa tggctttgaa catttcatgc tcaaagaaat
    34441 atttgaacag cccaaagcca taatggatac atgccgggga agggtttcaa taggtgaaat
    34501 gaaaatccat ttaggcggat tatatacagt aatgcctcaa ttgattgatg caaaacgaat
    34561 catcatcata ggttgcggaa cttcatggca tgccggctta gtaggcgagt acctatttga
    34621 agatttggca agaattccgg tagaagtaga atatgcctca gaattcaggt atcgaaaccc
    34681 tgttatttac aaagatgata ttgtgatagc cattagtcaa agtggcgaaa cagccgatac
    34741 acttgctgct attcagttag ctaaagctga aggagcaact gttttgggca tttgtaacgt
    34801 tgtaggttca agcattcccc gaaataccca tgcaggagtt tatacccatg caggccctga
    34861 aattggtgta gcctcaacaa aagcctttac ctgccaggta acagtgctta ccatgatggc
    34921 tatgcttatt ggcaaagaaa aaggggtgat tcctatggaa aaatatcaca gcctgattgc
    34981 ccaattagct gaagtccccg aaaaaattga acagatattg aacaatagca gtgctattga
    35041 gaaaatagct gaaatatatc atgaagcaac aaactcatta tatttaggcc gtggatatat
    35101 gttccctgtt gcgttggaag gtgcattaaa acttaaagaa atatcttata tccatgcaga
    35161 aggttatcct gctgccgaaa tgaagcacgg gcctattgcc cttatcgacg aaagcatgcc
    35221 tgtagtattt attgctacac gggataagtc ttatgaaaaa attctttcga atatccaaga
    35281 agtaaaagca cgtaaaggtg ttattattgc tattgtttcg gaaggtgata cagtaataag
    35341 gtctttggcc gaccatgtga ttgaaattcc ttattgtgaa gaaatgctta tgcccttgct
    35401 gacagtaata ccacttcagt tgctttcata ctacattgcc gtaaaacgtg gttgtaatgt
    35461 tgaccaaccc cgcaaccttg caaaatcagt aacagtagaa taaagcacta ttctgtaaga
    35521 gctgtattaa tgaacctgat aaaaggcgcc atgatggcaa actgttctat aatcttttca
    35581 atcattttag gagaagccaa caacttatca tcaaaggaag aagaaactgt atagcttttg
    35641 tatttgaggt aatcgatatg ctcaaagtct ttaggaaagc ctaaaggggc tttttgtaac
    35701 ttcatatcat tgtccagaga aggaaatgct tttttgaaat ttttgttttc aacaatttca
    35761 agaaactcat caatgttgtc gtaaatttct ttacgcagct tcttcaatac aggtggcatg
    35821 ggcatgtaaa cgcctccagc cagcatgtta ccgcctggct cgacatgaaa ataatacccg
    35881 gcaagcccgc tattctttcc tcctttaacc atgtaagccc ccatgtgggt tttatacgga
    35941 gttttatcgg cagaaaacct aatatcgcga taaatacggt aagtacactg cgaaacttca
    36001 agaaaggcaa tatctttatc aatttttccc agctcagaaa tcaaattcat ggtaacttgc
    36061 tcaaactcag ttttagcatt gttgtaccaa tctttgttag ccaaatacca ctcacggtta
    36121 ttgttttctt ttatggcctt tagaaaagcc tgaacatggt gcatcatttg cttaattttt
    36181 aatcaaaccc aaccaatcaa atataagtgt atttattgtc aaacgaaaag agttagatta
    36241 aattatccca aattaatcat taggaggctt tttaaagccg aactatatga tttcatctcg
    36301 ttataaacgg gacaaactga ttaagcattt tttttaatgc ttaatttggg attatgaaat
    36361 tctgacactt gattttgtct tattaaaaaa ataaagccca tttaatcaaa tgattaaatg
    36421 ggtttcttgg tagccccgcc aggaatcgaa cctgaattta aagtttagga aacttccgtt
    36481 ctatccattg aactacaggg cctaatgtaa gtgacaaatt atccaatgac aaatgacaaa
    36541 cttaaataaa atgtcaaatt ttgaggtgca aatataaatg gatttgtttt tataatcgct
    36601 acaaaaggaa tttttctcct ttaattaaac gttccggcaa gatacatcct ggttttttct
    36661 tcttttgggt tgttaaaaaa ctcttcagca gtgccatgtt caacaacctc gcctaaatac
    36721 ataaacacca cataatctgc tatacgttta gcctgacgta atatatgcgt aacaacaaca
    36781 agtgtgtagt tatttttcag ctccagaaac ttttgttcaa ttgcctggct ggatattgga
    36841 tcaagtgccg acgttggttc atcggccagt attattttgg ggtcaacagc cagcccacgt
    36901 gccaggcaca atcgttgttg ctgtccaata gataatgccg aggccggttc attcaaacgg
    36961 tctttaactt catcccacaa acttacctgt ttcaggtaat gttcaacacg tctggcaaga
    37021 atggctttac gcttgcgccc gctcatacgc aacccaaaag caacattgtc aaaaatgctc
    37081 attggaagcg gaaaaggacg ctgggccaat aaccccattt gtttacgaat ttctgtaata
    37141 tcgcggtttg tattgagtat atcttcttga ttaataaaaa tattgccttt tacatgcaca
    37201 tcaggattga ggtcggtaag cctgttgaga gttttcaaca aggtggtttt accgcaacct
    37261 gatgggccaa taataacagt aattttattg gttggcaaat caagattaat gtttttcagg
    37321 atttgttgtt tgccaacgga caaattcagc tcacgtattg atattagcga ttcctgggtc
    37381 attttatact gttttttttg aatttatgcg aaaagtacct tgacaaaagg ctgatgatta
    37441 agactatgaa agttaaaagt accgcggctg cataagccct ggcttttact tcttcaatgg
    37501 gcgaactaag ctgaaagaat atggacaaag gcaatgttgc tgttggttgc atcagcccag
    37561 taggtatcct gtcggtaaat cctgcagtaa atagcacagc tgcagcatcc ccaatagccc
    37621 tgccaaaaga tagcagtaat gctgtaacaa tcccaggcat acattgacga accataatct
    37681 tgtaacctac ttcagtacgg tttgctccta aagacaatga agattctaac aatccacgag
    37741 gtacagtttt caagacttcg tctattcccc tgatgataat aggtataata aacaaggcga
    37801 ctgtaatgat tccggcaagc aatgaagatt tcaatcctaa ccatatcatc aacataaagc
    37861 caaacgcgcc ataaacaatt gaaggtaccc cccaaagcaa gtcaagcaag aaacgcacag
    37921 catgaacaaa gcgttgatat tgcactacat ggatattgat aaaaagagct accggtaacc
    37981 caataataat agccagtata acagccccaa atgacaaata aacagaacca acaatagcat
    38041 tggcaatacc tccctcatga ccaaagtaat aaccgccttt aggaacagag gtaagcattt
    38101 cccaactcaa tacccaaagc cccttgtgta atataccgta caaaataaga agcaatacaa
    38161 tactaataag tatcgtagat acaaacatca gcaccctgaa aacattctct tctattttat
    38221 tgcgtttttt cattttcttc attttaccac ccctacccct cctttgaaag gaggggaaag
    38281 atttctccta attcactgtt tctgatcccc tcctttacaa ggggaggtta ggaggggcta
    38341 tataatccgt aatttgtcat ccgtaattag ttatattttc ttctcaaacc tgataatcgt
    38401 caacctcgac accaaattaa acagaagcac aataacaaac aataccaaag ccgaaagcat
    38461 caaagccgag tcataaagtg gaatagagag catctcgcca tagttgttag ccaataatgc
    38521 cggcaacgga taacctggct gaaatatacc ttcaggaatt ttaactacat tgccggttac
    38581 catcagaact gcaatagttt caccaaaagc acgtgaaatg cctaatccta tagcagaaat
    38641 tattccggga aaagcttttc gcaggataac tttttttata gcctgccatc ggcttgctcc
    38701 taaagacaat gcagcttcgg tcagttcttc aggtactgtc tgaaatattt caatcatcat
    38761 attcagaaca aaaggcaaaa tcataatagc caacactatt gaacctgcca gaatgctata
    38821 gcccgaaact gtatagccaa agagcttagc aatatattgc actgccggaa caacagcaat
    38881 tactccccat ataccaaaaa taactgacgg aatgcctgcc agaatatcaa tcaccgggtg
    38941 agtgactttt agcacccatt tttttgcata ttgtgttaag tgaatagcag aaagcaaact
    39001 tataggcaac actataacaa tagcaaagaa agttacaaat agcgaactgg ttataaaagg
    39061 caataaacca aatttgctac tctgtggctt ccattccgat gaaaacagta tgtccttgat
    39121 agggtattca tggagtataa agctggactt atatatgaga ccagccccaa tgatgaaagg
    39181 aagcaagata actattgcaa tagcaattat catccatgtt tgatgcaggt tgtccttaac
    39241 ttttctcaac ctcattgggg ttgtataatt attttagact atcattacta cttaatcctt
    39301 tacgaagaac aaacctgact ttagaaagtt catctattcg ttttttttct gatagttcaa
    39361 cataacctgc catcttaaca tattgttgtc catcagtaag tacccacttc agaaattctt
    39421 ttactacctt attttcaggt ttacctttac ttacaaagta cagttcgcgg gcaggaggcg
    39481 aagggaactt cccttcacgg atggctgcgc taaggctatc aaggttttga taaaaatgtt
    39541 cttctttgtc aataatcctg ttttcgttca ggtcaagagg caccacttct attcctttat
    39601 tataatgcct cgatttaatg tcataaacat aaatgatatt gttatagccc aatgacaaag
    39661 gttcattttt aacagcatca gccattcccg gatctccaaa aacacctacc ccttgtaatg
    39721 cttcctgatt catgccaaga taacgtccaa acatttctcc ggcaccacaa gcatcagaac
    39781 gggtaaataa atgcacatga gccttagtat tgccattata aattctttgc cagtcagaca
    39841 acctcccatc aataaagatg gctttgagtt gtttttgcgt catccctctt ttacggatac
    39901 tgtctgcata agggtttgat gagctaaaaa caggaacaac agcatctttt acaacaccaa
    39961 tataccatgc gcctctgttt atttcagctt cactcaacgg ccttgaaacc attccaaggt
    40021 caaccatacc actcaaggca tctgtcattc ctttgcctgc accaccagca gaaacgtcaa
    40081 tagttacgtt tgggtataat ttcataaact cttctgccca tttggcagtg attggatata
    40141 gggcaaaagc cccggagatg gttatagaac cttctaaatc actttttttg tgatatttcg
    40201 gattgttcgt gcaagatgaa atgataaata ccacaaataa tatccataaa aatagtgtct
    40261 tgtttatcat gattaattaa tattaaggct attgaagtca ataaaagtaa ccttttatga
    40321 aaatcattgt ttaaaataaa tattatctca aattaaacat taggagtcaa aattttgccg
    40381 ataatggaat ttattaaatt actaaattac aatttcttaa tcgattaagt attttctaat
    40441 acttaatttt ggataattta agaaacaaag ataaaaatag tttaatatct tttaatattt
    40501 aatttattta aatccagaaa taaattacga aatctaaatt tcagaagcag ctaaaaaact
    40561 ttctttttag aaatagtagc aacaaaatct tttagataaa atggttcaaa ataggctaca
    40621 tcttcaactt cattggcctc aaattttgct aaagcagctt tgcacataga acgggcatga
    40681 ggcctgatgt tgttgacaaa aaaagcattg tctgatacaa tgatgttagg aagtttgtcg
    40741 gcaccgcttc ccaaaaagta tattctcttt tctttgagca aattatcata agaggtacta
    40801 ttgattactt cagcctcaat gtcagatgta gcattaaaag ctgaatcaaa aaacctcctg
    40861 taaacttcca tcctacgtgc atctatcata gcacaaatca tactttcagc cgagtcaaaa
    40921 gtctttttat ttttagaata aaattcggaa ataataaact cattgaccaa aatggtaaat
    40981 gtatcaacag caattaaagg tatttttaat gaatagcaaa taccttttgc cgtagaaaca
    41041 ccaatccgca aacctgtata tgaaccaggc cctttgccaa cagcaacagc attaagttgt
    41101 tcatagctaa ttttgcaatt gtgtaatagt ttatcaatca gcaatgtcag gctcgatgca
    41161 tgcgacttat cttcagtgga tgcttcgctg cccaatattt gcaaaccgtc tgcaagggca
    41221 acactacaaa gtttttctga tgtttctata gaaagaatta aaggcatctg cgcatctgct
    41281 tatttatttg aaaaaagcat ttttttatct accaccttca caacatcacc atccttcagt
    41341 ttgcgggata ctgcactata aggggcaacc actactttat ctttttcaga caaaccggct
    41401 aaaatttcta taaaattgtt atcctgaatc cccgtcttaa ctttcttcag cttgactatc
    41461 gattggccgt tcaaaacaaa aacagcttcc ttgctgtcaa tttctttttc ttcttttttc
    41521 tttgttttat catcattatt ttgcattgcc gaatccgatc ttgaggtaat tgcttcaatt
    41581 ggtactgaca atacgttagt acgatactct gtttggatat caacattggc agtcatcccg
    41641 ggtctaaaag gatatttgcg gtcagcatta ataaggtctt tatatgactc tggcagcatc
    41701 aatattttta cttcaaatga agtcacctgg tcagacatag cactggatga cgaactcgcc
    41761 gaattggcaa tttctgatac taccccttta aacttattcc ctaaaaaggc atctacttca
    41821 ataatggcgg tatcattaac agcaacacgc acaatatcgt tttcgtttac ttcaaccttt
    41881 acttccattt tgttcaggtc ggctacccgc atcagctcag tacctgccat catttcagta
    41941 ccaacaacac gttcgccttt ctcgacattt aaacgggtga caataccagt catcggagca
    42001 taaatagaag ttttgtaaag gttttcttct gcttcggtaa ggctggcttc tgcgcttttt
    42061 aggttgtatt ttgatgattg aacatttttt tctgccgcta aaagttcggc tttatttgat
    42121 tcaaaatcag tttttgccgt ttcccactcg tgctgggata tagccttttg ttcccaaagt
    42181 ttttcgttac gctcatagtt tagcttagat ttgttaaata gtgcttcgac ctgcatttta
    42241 cgggcttcag cgttttcaag accggccttg gcagaattga caacagctgt agccctgtct
    42301 ctggccgaaa tataaatgtt aggttttatt tttaacatta attggccttt ttcaactttt
    42361 tgcccctctt ctacatatac ttcaattatt tcacccgaaa catcagcact gatttttact
    42421 tctgtttccg gttctatttt gccatttgca gtcaccaatt catatatcgt ccgttttttt
    42481 acctcttcaa cagcaacttc aacaggcttt tcttttccta tcaatccggc tttgcgtcca
    42541 actatagcaa taagtattaa aaaaataaca attgataata gtatccagtt ttttttcgac
    42601 atattcatgt ttttgtgctt aaaatagtca acgaaaataa aaagaaatat gttcttttgt
    42661 tctaaattct tcttaatttt tgaaaaaggc attacatatc atctcagaca ttgggttggt
    42721 cgaaattatc aaacgtcgcc aaaacaggcg tattattgtc aaatcgggca accatcaggt
    42781 ctgcctgaga gtcagtgtcc cttattatgt ttcgtttgat gaagcccttg gttttgtcca
    42841 taaaaatatt gatacaatac gtaataaact catcaacctc aaaagccatt ctataatacc
    42901 tgctgttata aatgaaggtt tcaattataa aaatgccttt ttttcggtta aaatcgaaag
    42961 agttttacca ttatcaccga aaaaagaagt tgagctttgt cagaatggcc gcgaatatat
    43021 catcagagtg gatgaaaaat tagatttgca gaatgaaaag atgcaggaaa aattactttt
    43081 ttattttgag aagattatcc gcaaagaagc taagcagtat ctgccagata ggctaaaaca
    43141 actttcagca ttacataaca tcccttttca cagtgtcaaa atcaacaatg cccgtacccg
    43201 gtggggcagt tgttcttcaa ataaccagat aaaccttaca ctaaagctta tgcttttgcc
    43261 tgcacacctt atagattatg tattattgca tgagttagcc catgtagtac ataaaaacca
    43321 cagtccaaaa ttttgggctt ttttgtcaag tttgacagat gatattttgc aaaaaagaaa
    43381 tgaattaaaa aagtataatt tataaaatat tgattattaa taagttacta tattgctatg
    43441 acaaattgtt aaaaaaatat aataaaaaaa ttgcctttta aaaaaaaaat atgaaatttg
    43501 ttttcacaat tatctatttc aattattttt gcaacgttat taaatttaaa aactaatgaa
    43561 aaagttattt ttgttatcag ttgttgcagg attagtaatc actgcatgtg gaccaagtgc
    43621 tgaagaacgt gaagcacaaa gaatcgctga ctctacaaga caagctgact ctattgctgc
    43681 agttgaagct gcacaaaaag ctcaagctga ctctattgct gctgctgctg acagcgctgc
    43741 taaagctgct gaagctgcta aaggcaaaaa gaaataattc tggtagagca gaaaaaacaa
    43801 aggagtgcaa aagcactcct ttttttatat tttaattggc tttaattttt ttctctgtac
    43861 tggtgctgtt ttcctgatcg atagcaacca cctcctgcca gttatcccct cttttgatac
    43921 gcgttatttg catttcgcta acgcaaaaca ttttggcaat taacttttgt tttactcccc
    43981 gctgtaacat tgattttatc aaggcaactt catctgtttt cagttttgaa taagtaacca
    44041 cttttccttt tttcccgact ttgacggttt gattttataa aacaaagata ttcaaatagt
    44101 tatt
//
LOCUS       DAOSPF010000027        43125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_931_length_43125_cov_6.057627, whole genome shotgun sequence.
ACCESSION   DAOSPF010000027 DAOSPF010000000
VERSION     DAOSPF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 43125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_43125_cov_6.057627"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /gene="alaS"
                     /locus_tag="PK252_07185"
     CDS             <1..1873
                     /gene="alaS"
                     /locus_tag="PK252_07185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT14529.1"
                     /translation="KKSNYDTDVFTPIIEEISRLTGIKYGNENMTDIAMRVVADHLRA
                     ISFAIADGQLPSNNKAGYVIRRILRRAVRYAYSYLNQHGPFICKLVPALTANMGNAFP
                     ELKAQQKLIVQVIAEEETAFLRTLETGIKLLDQLILKAKAAKTSVIDGKSAFELYDTY
                     GFPLDLTELILREQSMSVNREEFDQQMEMQKSRSRNDAAVETDDWQVVSSKDTTEFVG
                     YDTIEADVKIVRYRKVTTKGKTQYHLVFDKTPFYAESGGQVGDMGTITANGEVVQIID
                     TKKENNLSVHICEQLPSFLSATFKAKVDETKRLDTASNHSATHLMHYALRQVLGKHVE
                     QKGSLVTPEYLRFDFSHFQKLSPEEIAKVEEMVNLQIRKNIALNENRAMSFDEARKTG
                     AMALFGEKYGDKVRVIKFGDSVELCGGTHVAATGQIGSFKIISETAIAAGIRRIEAIT
                     GNGFMQYVHKNEELISSLKEMLGNPQDLRKAMEKLLTDYSGLQRKIEQAQKQQLDIAV
                     QQLQKKVKNINGIEVIAETVEADSAQSLKDIAFRLRSHAENAYLVLGAVIDDKPTLAI
                     AISDKLVATKKLNAGQIIREVAKLIDGGGGGQPFLATAGGKNAAGLSQAIEKSLTFI"
     gene            1933..3714
                     /locus_tag="PK252_07190"
     CDS             1933..3714
                     /locus_tag="PK252_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT14530.1"
                     /translation="MNVTRVFDLFEHIKKEYNKADVFGYKKQGVWHTYHIDQCIELAN
                     LLSSGLLSLGLKKGDRIVSISNNRPEWNFLDFGMSQIGVIHVPVYPTISNDDFHYIFN
                     HSAPKCIFVSEQSVFNKIEPVAKQCGITDIYSFDGLNGTVNWNYILDLGRAHIDEHKA
                     EIKLLKDSILPSDVFTIIYTSGTTGLSKGVMLSHDNFVQNVKNSVHIHDVDHHCRSLS
                     FLPLSHVYERMLNYHYQYKGIGIYYAENFGTIADNLKEVQPHIFCTVPRILELFYEKI
                     VSKGYELSFVSRLLFFWAIHFGKKYEVMKKKSLLTKLKRKIADKLVYEKIRMSLGGNL
                     RVVVSGGASLQSSLARLFWAAGIQLLEGYGLTETSPVISVNRLLPKVETYPGTVGKVL
                     DGVEVKIAPDGEILCRGHNIMLGYYKQPELTAEAIDKDGWFHTGDIGFFTPEGFLKIT
                     DRKKEIFKLSSGKYIAPQVIETKLKELEYIEQAIVIGENEKFASALVVPNFYHLHKWS
                     SQHHIIFRDNEELIKNPEINKLYQKLVANINLQLGLVEQIKRFRLVHQEWTPQTGELS
                     ATLKLKRRVILEKYSDIIEQIYSASKN"
     gene            3876..4910
                     /gene="asnA"
                     /locus_tag="PK252_07195"
     CDS             3876..4910
                     /gene="asnA"
                     /locus_tag="PK252_07195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOT14531.1"
                     /translation="MKLYIPKNYKPVLNLIQTEKAIKLVKDFFESNLSSELRLRRVTA
                     PLFVLKGTGINDDLNGVERKVTFPIADMKEQQAEVVNSLAKWKRMMLADYNIPQGYGL
                     YTDMNAIRPDEELDNIHSLYVDQWDWERSMSAEERNVDFLKYIVRKIYSVLKRVEYVV
                     YEEYNQIKPILPEEITFIHSEELLKMYLNLSAKERETEFAKKHGAIFIIGIGDKLSNG
                     EPHDGRAPDYDDWTTKSENGFNGLNGDIILWNPVLEQAFEVSSMGIRVNKEALLKQLT
                     IAKAEDRKELMFHKRLLNDELPLSIGGGIGQSRICMFFLRKAHIGEIQSSIWPKEMVD
                     ECKKNQIILI"
     gene            5002..6387
                     /gene="glmM"
                     /locus_tag="PK252_07200"
     CDS             5002..6387
                     /gene="glmM"
                     /locus_tag="PK252_07200"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT14532.1"
                     /translation="MTLIKSISGIRGTIGGKPGDNLTPLDIVKFSAAFGTWLIQQNKQ
                     AKCTVVVGRDARISGPMMDHIICGTLVSLGIDVINIGLAATPTVEIAVTETKSNGGII
                     ITASHNPKQWNALKLLNEKGEFINDTQGRLVLQLAETEQFNFVDVDKLGKVSYRHDFN
                     DIHIQKILELPLVDKDAIAKAGFKVAIDCVNSVGGVVLPQLLKALGVTEVLPLYCNPD
                     GQFPHNPEPLPEHLTEISALVKDKKANLGFVVDPDVDRLAIINEDGSMFGEEYSLVAV
                     SEYILSKTPGNTVSNLSSTQALKDVTVKFGGTYTASAVGEVNVVTAMKQTNAVIGGEG
                     NGGVIYPELHYGRDALVGIALFLTYLAYKKMSCSQLRSTYPSYVISKNKIELKPGIDV
                     DAILANIKDKYKSKPIIDVDGVRINFENQWVHLRKSNTEPIIRIYAESKTMDDANNLA
                     QSIMNDIKAMS"
     gene            complement(6492..7349)
                     /locus_tag="PK252_07205"
     CDS             complement(6492..7349)
                     /locus_tag="PK252_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14533.1"
                     /translation="MSKIGLFCTPDTNETLINSFLNAKNIHHSAICIDLSSNAPEQAN
                     STNPFLLISNSDILIFDVSQPEWYNLAIESIQQSKHIILLNPYFLRHEQIKYLQMLSI
                     EAEIMIQPHISPKAIALSKLFKNTSYLKIEIYTNISFWEKHEYNKHYTTICDIVAALM
                     HGKIKKCYSYSSRHNNKEMSINGTYISFINGNSVHIEINTNNKDSFNFQIKICDEEKT
                     GSLEWTDNAINLIYYKTGSTITENIEYKAESFLNEAINGTITGIHAYKITDFLSALRI
                     FEELKKNAS"
     gene            7589..8230
                     /locus_tag="PK252_07210"
     CDS             7589..8230
                     /locus_tag="PK252_07210"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT14534.1"
                     /translation="MEDTFRHKGLRNKLVECLRLKGIRDEKVLLAINKVPRHLFLESS
                     LINHAYQDKALPIGAGQTISQPYTVAVQSELLKVKPFEKVLEIGTGSGYQACVLLEMS
                     ARVYTIERQRELYLKAMSFLPQIGYNPQFFYGDGYLGLPTYGPFDKIIITAGAPEVPK
                     ALLEQLKIGGRLVAPIGDSQVQVMTVVERKGEDSFVTTHHGSFIFVPLLKGTE"
     gene            8231..8875
                     /locus_tag="PK252_07215"
     CDS             8231..8875
                     /locus_tag="PK252_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT14535.1"
                     /translation="MNSYYTLTYNPFGVNTYVICNEQNECLIVDAACYDKRELEHLYN
                     FIEGKKLKIVAAVNTHGHFDHLFGVDEVCSKYNLTPYIHQDDMYVVKAAAELAIPFGL
                     VVRSLPPKWNYFTEDGMVSIGGFNFRVMQVPGHSKGSVVLYFEKYKILVTGDVLFAGS
                     IGRTDLPGGSYIDLISGIKSKILNLPLDTVVLPGHGPSTTIGEEISSNSFLNYL"
     gene            8885..11761
                     /gene="gcvP"
                     /locus_tag="PK252_07220"
                     /pseudo
     CDS             8885..11761
                     /gene="gcvP"
                     /locus_tag="PK252_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
     gene            11907..12509
                     /locus_tag="PK252_07225"
     CDS             11907..12509
                     /locus_tag="PK252_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT14536.1"
                     /translation="MEQFSNEDIIGGILNNNSKVLRYIYAENFPKVRQHICINGGSED
                     EAADVFQEAILIIFEKARKEQLVLTVKFSTYLYAVAKVVWKSYLRENRKEEDMFQLNE
                     TEWDFEIGEVDDYIIDNERFKLVWRYFDNISKDCQTIIRLFLEGKSISEVTQEMNYSS
                     EQHTKNRRLRCKESLIKKIMADPKYKELCREIKIDKINRW"
     gene            12738..13433
                     /locus_tag="PK252_07230"
     CDS             12738..13433
                     /locus_tag="PK252_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14537.1"
                     /translation="MMKFENENDLKLYSDYEHDVDTFLSDVEKKRLIDNLQTIQVEYI
                     ESQHDDKKTPIRLSRKKYYFVAVAMLILLIGVAGVLKFSILKTTIEGTDIFLQYYSVY
                     KVDGVMRSDINANSELSLALEAYSEGNFGKASSLLKSHAYSGIEFNKAQFYFGLASIE
                     LADYQVAIEAFNKVLSNGNNPYIAQSHWYLALTWLKLNNYNAAKEHLQWLQQNDRFYG
                     KKAKEIFNKLNDN"
     gene            14055..14645
                     /locus_tag="PK252_07235"
     CDS             14055..14645
                     /locus_tag="PK252_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOT14538.1"
                     /translation="MRSEIDYFKIEANHVAPEQGNILIAEPFLADNYFKRSIVYLVGH
                     GVEGSVGFILNKPVSMKIVDLIPDFPSNDFSLSLGGPVSPNTIHYIHTLGDIIPDSVR
                     VKDDIYWSGDFDVVKQLIAAKQINKSQIRFFIGYSGWSPGQLENEIDQNSWLIHHSNN
                     NFIMKNDKEHAWSKILYDMGGKYIMWANSPENPSMN"
     gene            complement(14742..15602)
                     /locus_tag="PK252_07240"
     CDS             complement(14742..15602)
                     /locus_tag="PK252_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT14539.1"
                     /translation="MAANDTFIIVDGNIYRSDEQLFNHNNRSFCFGDGLFESMHAYGT
                     DVQLFNLHFERLTNGIKALKMQPDKSFTPAAISKQITHLLNKNKLFTGARVKVILFRK
                     DGGRFLPETNDFSYIIETKQLEQPYYLLNKKGCSIEIFKDITKTASSISSFKTTSSAI
                     YVMASIFAHENGYDDCLILNQKQELIESCNSNLFVVKNNVLLTPAADSGCVSGVMRRY
                     LMTIAKEAGINVIDNIPITESILLEADEIFLTNAISGIRWVQAFRNRRYYNAMSQNLI
                     KLLNKSVFGV"
     gene            complement(15589..16092)
                     /locus_tag="PK252_07245"
     CDS             complement(15589..16092)
                     /locus_tag="PK252_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOT14540.1"
                     /translation="MNKAVFIDRDGVINNDEGLYYIYNVNDFRLNENIESNITKLCKA
                     GFLVIIISNQGGIGKGLYTIAHTEELHKILCEKVKEKGGTVTELYFCPHHEQSGRCLC
                     RKPDSLLLEKAIARFNIDKNMAFFIGDSKRDVEAADKANIKGILVPKNTDIAAFVDSI
                     IKEYGSK"
     gene            complement(16102..16728)
                     /locus_tag="PK252_07250"
     CDS             complement(16102..16728)
                     /locus_tag="PK252_07250"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT14541.1"
                     /translation="MTVFLVVATSIISTIAFYNQQLFELFELNPYKVVHRKQYWRLIT
                     HAFIHADWMHLIFNMIVLYSFGLGLEYYLSILKGMVSLRFPVVAYYMTMYFTAIVVAS
                     LTTLKKYKDTFIYNSVGASGAVSTIVFACIFFNPWQKLLFLGIIPIPGIIFGVLYLIY
                     SQYMSKRNHDNINHDAHLLGALYGFTFPILLEPKLLYGFIEQVFNFSF"
     gene            16897..17325
                     /locus_tag="PK252_07255"
     CDS             16897..17325
                     /locus_tag="PK252_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOT14542.1"
                     /translation="MPYKEKQEQFDRRYLEMARIWAKNSYCKRRQVGALIVKDKMIIS
                     DGYNGTPSGFENNCEDESGRTYPYVLHAEANAITKVAKSNNSSENATLYITTSPCLEC
                     AKLIIQSGIIRVVFADKYHQEDGINLLKKAGIEIVHIKFE"
     gene            17327..18940
                     /locus_tag="PK252_07260"
     CDS             17327..18940
                     /locus_tag="PK252_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT14543.1"
                     /translation="MSVENMNRRKIYLPLIISVSIVVGILLGSFLTNRIQSGRNSTLM
                     FPSSGKLNNILQYIEEEYVDTVSSDRLTESAIVSMLKELDPHSAYIPASELQAVTDPL
                     EGNFSGIGVQFNMQNDTVVIMNTVPNGPSALLGIQAGDRIVKVNDTVVAGVKMESDAI
                     VRKLKGPRGTKVQVSILRPGNKKPILYEITRDIIPLNSVDVAYMVTPKTGYIKINQFA
                     RTTPEEFVKAVTNLRNLGMQNLVVDLRGNGGGYLDAAINLADQFLPEGRLIVYTKGRA
                     KARENFTATYSGVCEGNEIAILIDELSASASEILAGAIQDNDRGTIIGRRSFGKGLVQ
                     EQIPLSDGSALRLTVARYYTPSGRCIQKPYHKGDEDYFLDITKRYQHGEFENKDSIKL
                     NDSLKYTTLKGRVVYGGGGIMPDIFVPLDTAGFSRFYEQIRSNGLIYRFSFKYSDNNR
                     QRLKSIANLDALRKYLREDKVFMKFIDFASEQGVKASTRDIEVSKKYISTELEAFIAR
                     YFFDDEGFYPIIGEIDNTLSKAIEVLEKK"
     gene            complement(19257..20006)
                     /locus_tag="PK252_07265"
     CDS             complement(19257..20006)
                     /locus_tag="PK252_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT14544.1"
                     /translation="MNVIVIEDEKPAADYLQSLIQDIDPNISILAHLDSVKKAVSWLS
                     KNSCDLIFLDVQLSDGISFEIFEQIKVETPIIFTTAYDHYAIKAFKVNSIDYLLKPIN
                     IEDLRQAIQKYKTRAAESPAPDFSVLFESLKKGPSYQERFLVSAGTKLKSIKTENIAY
                     FYILENGTFFVTKENRHYLVSQTLEKLEEVLDPKHFFRINRQCIVHIDAIESMEVVSK
                     SRLKLTLSPKADFETIVSVNNLHDFRIWVNK"
     gene            complement(20003..21058)
                     /locus_tag="PK252_07270"
     CDS             complement(20003..21058)
                     /locus_tag="PK252_07270"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT14545.1"
                     /translation="MHLTRKVIIKAIALVCICTTIIIPSVYLISNWLNNTEIVVNEIL
                     LNITFTFVVTLSITSANLFTFRFFENKYAGHKGYWRIFLVEMFITSVNAAVIISIIVY
                     LFYIVLGEFPKGQNEKVAALFFENIIVALIVNAIACGLAEAYIIFRRWKETLILTERL
                     QREKAESQYIALKSQVNPHFLFNSLNTLSSLIRTSPDKAIGFVDKFSKIYRYVLDSSD
                     KMLITVRQELDFINSYIFLQKIRFGDAINIDIQIESEYMNDFIPPLSLQLLVENAFKH
                     NEASQEAPLQVKIFVENSNIVVMNNLQMKRIRPESTGIGLKNLQARYNQLCDAMPEFY
                     TTEKQYIAKIPLIKDEQ"
     gene            complement(21070..23502)
                     /locus_tag="PK252_07275"
     CDS             complement(21070..23502)
                     /locus_tag="PK252_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14546.1"
                     /translation="MTNLKNILLCITFFVASITISAQNLKPAIVQTIKGRAIDKVTGQ
                     GLPGASVKVEFTGGETGVMADEEGYFKVTDIPVGRVTLSISYLGYNQATFSGLSVISG
                     KELVINAEMEEKVTVTDEVIVTAAKSKARANNEMAVVSSRMFTVEETERYAGSRGDVA
                     RMASNFAGVSFANDSRNDIIIRGNSPSGLLWKLEDTEIPNPNHFAENGTTGGPVGMLN
                     NNVLKNSDFMTGAFAAEYGNALSGVFDLKMRNGNTDKYEFLGQVGFNGFEAGAEGPIS
                     KKNKSSFLINYRYSTLDMMYKMGFSFGTSGVPKYQDLSFKANYPIKNGVITCFGIAGK
                     SSIEMFDSKQDEAKDDMYTSSGQDLTNYSSMAASGISLTLFPDTRKVLKISLSGLAQE
                     GGTNIDTLQQFKNIVNTDTFYYYRIPTRTLEHYISEYRTTATASLANKYNAKFNTKLG
                     ISIDQMGYNLDTRIYLTKNVPQPGLYQAMDASKSLFEGPQLYKAFYEASIKITDKITF
                     NPGLYATLFSLNNSKALEPRAASIFEYSNGKKINIGYGLHSKTHPLSVYYLVEADAPT
                     RAETNKNLDFTKAHHFVIGHDWNINKDFRLKTEAYYQYLFNVPVETKSSSFSLLNSGA
                     SWGVNAEDSLVNKGKGYNYGFELTFEKFLSRNYYFLVTTSLFDSKYKASDNKWRNTAF
                     NGNYVVNALVGYEHPVSQKWTLCFDIKSSFAGGKRYTEVDIAKSLQERDTKYKEDQAF
                     EKQYPMFFKLDVKFTIKNNNLRYSQEWQIFVENVTNHQNLLYEYFDSNTLRLKKVNQL
                     GIFPMMMWRINF"
     gene            23779..24864
                     /locus_tag="PK252_07280"
     CDS             23779..24864
                     /locus_tag="PK252_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOT14547.1"
                     /translation="MKKNIFTLVTLIMVTCAFSFGQSKKASIAFATEKYDFGKIQESA
                     GQVSYDFVFTNVGGEPLVISNVQASCGCTSPNWPKEPVMPGAKNKITVTYNPAGRPGK
                     FTKSITVTSNAEPASSTVFIMGEVIPKEPTIEDKFPQDLGKVRMKTTQFAFNAISPVK
                     VSTSSAELYNSSDSVVALGVKNVPNWMQVKIIPEKVQPKQIAVVEVTYDAKAKNDWGF
                     VVDKFDLTINGVPAKARMTVTGNIMDDFTTLTPEQKANAPKIVIDSAVYDFGTIKSGD
                     KAKHDFVVKNAGKSDLLIRKIATSCGCTATNLKSQVVKPGESTVIAVEFNSAGRTGNQ
                     AKQITVISNDPENPKMMLNIKGEVIAQ"
     gene            complement(24937..25746)
                     /gene="ispE"
                     /locus_tag="PK252_07285"
     CDS             complement(24937..25746)
                     /gene="ispE"
                     /locus_tag="PK252_07285"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT14548.1"
                     /translation="MVGFPNAKINIGLHVTSKRPDGYHNLESIFYPVNLTDAIEIVKT
                     DGHFSFTTTGITIDGKPENNLCIKAFKIMHNHYNITNVNMHLHKAIPFGAGLGGGSAD
                     AAWVIKMLNTTFDLNLNNKKLQEIALQLGSDCAFFIENKPSYVTGRGENIEPIDFSLK
                     GRYIVIVKPDIYISTPEAYKGIAPKPDRPALPDLIRQPVEMWKNIIVNDFEKHLFIQH
                     PTLSEIKQKLYEAGADYASMSGSGSAMFGLFRHEVDLKGLFHGMQVWCKEL"
     gene            26028..28139
                     /locus_tag="PK252_07290"
     CDS             26028..28139
                     /locus_tag="PK252_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOT14549.1"
                     /translation="MIKHLTLLIFILIGLKTSGQYKLVWEDNFDGTTLDSSKWGYVDE
                     IGVWNTGANNELQRYRKENVTVGSDGAGNNSLIITAKKESYDGYQFTSGRVDTRAKFS
                     FKYGKLVARIKVPDLANGLWPAFWTLGVTPDTWPACGEIDILEMGHAQGIASNTQNKY
                     LTGALHWESGGNQADYAKAYTAGKNLNEDYHLYTLEWTPMYIKMYLDSIATPYYTMNI
                     PSADGEEFTDYLHYVIFNLAVGGQLPGITNATGITASLPASMYVDWVKFYQKEGEGAV
                     NINDEAIWNNFGVFDETGSYFYKADIGFDAKIVVNSTFGKPSNYAVFEGDTALAYLLG
                     SNDSLSVSVNSLIKRNLILFNEGSICFYLKTNNSQPLTIAVNDFDGGSSFYTLSGFEA
                     DNTYHKVAVPVKGLKGNVNLSKIKSLFALSAKYPGAAEISIDKVFWSTSVPSAGNYLG
                     LYTENPNISEKLEVNNSSIFLYIWDNTLKVLTTNAYEGKNVMSFASVAGKTWYGTGLY
                     ANPAVDLSAYKNGSLHFALKTTDTKEFYIGIEGANRTKKTIIFNEPNDPYGFVRDGQW
                     HQIAIPMSEFSSVDLTACGNFFIMGGAPSITDIAVDDVYLSVDASSIENPAINTSVKN
                     AQSQMNDITVSPNPFHDVVNIKTTRPIDIIDLYTYDGKIAKSLKSNGNDVNINLSELQ
                     PGLYFLVVNKQRQSTRKLTKY"
     gene            28191..31754
                     /locus_tag="PK252_07295"
     CDS             28191..31754
                     /locus_tag="PK252_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14550.1"
                     /translation="MVKKMLLLVTFLAGLVQLYAQDRTITGKIVDEAGNILPGATVTL
                     KGTTKVTVTDLNGVYSLSAPQEGGTLVVSYVGYTSQEVQIGESNTVDINLQPDIQALN
                     EVVVVGYGVQKKSLVTGSIAKVKAEDLANQPALRVEQALQGKTSGVIFNQTSGNPGAQ
                     VTVRIRGVSSNGKSEPLFIIDGMKTSKFVMNEIDPADIESVEVLKDAASAAIYGSEGA
                     NGVIIITTKSGAGNKEKVTVTYDGYYGWQVADPVAVMSTAQYKDYFREAYAYDKSIEA
                     SNSIKSFALPAFVEKVSYWTDAADSLIFKYNTPAYQYERVRSIAEPALQKALTNQQTL
                     LSNGSITQKQYDNNVKAINNKLEALANLRPLTFEQYKANLFNKKLNSPTDKAYANIRD
                     NVDTTSNGTNWMDEIFQVAPMQKHHISVQASSEKANLYLSASYFTQDGVVGGAKNNFT
                     RYNFRLNGDVQATKWMKLGSNVTVAQSKSRNLPLNDLYGGIISAAMSHDPTIVPYWND
                     TSEVFAYYRSVGRNYTTEAAREDLLNNRLLKTEDGRYYSNTTMPVNERVNPLAIMQLS
                     QNDVATTERVIGNAYSEIKPFKFLTYRLSFSGELTYVTNDNWGPAYKLNDTRQRDYSY
                     VYKQIARYYNSQIDNTLSFVKSFGDHNVSATVGQSAEQYIGYSVNGTRINLQELNESW
                     NYLDAVQRPDTLSKLRDDNGGFKDKNTKMAYFGRVNYDFREKYMFGATIRRDGSSLFS
                     DKKRWGVFPSFSLGWNVHKESFFNVPFVSLMKVRGSWGRNGSTQNVDAYMWISTKTAQ
                     SLGGAKTIVTGVTKNEDLKWETSEQTDIGVDFGFLKNKLTLTIDWYNKKTIDLLAKPI
                     LPAWMASSSGDVAYYNVGEVSNKGLEFDLGYSDNIADFKYDAKLTATYLKNEVTDFNA
                     QRLTGYGAHGAPFEATAFEEGQPVWYFRGYKAEGIFQSWEEIRNYTYTDPNTGVVTLI
                     QPDAVPGDVKIKDVGGTVDPKTGKASIPDGKIDGNDYTYVGKPMPSWTFGLNLNGEYK
                     GFDLSMFWYAEVDKEIYNNTLRGDFLHLNRAETFYTNRWTEGSGTNDWMRARYTDNTT
                     GSTQIGFNSLFFEDGSFLRLKNIQLGYTLPVSLTQKVQISKLRFYVSASNLLTFTNYT
                     GSDPEIGYTGDNNSKNHNSFGVDRGMYPSAKQYIIGVNITF"
     gene            31773..33335
                     /locus_tag="PK252_07300"
     CDS             31773..33335
                     /locus_tag="PK252_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14551.1"
                     /translation="MKTIYNTFLILFSCMAIFSSCSEDRLDVEPFGKYAQDPDPNRPL
                     DLNMIDGEARGLYSQVSWCYNTQWGYSTYVTFNSASDDAVSGGAAAGDRPEYEAAEKF
                     TLDPNNLGVINLWKRFYSGIGSCNKLLDNYPDKDIDTLKRAKAEAKFLRAYYYFNLVN
                     IFGPVPMPLKSTDLAEMPNTPIAEIYAQIEKDLNDAIASNGLPKSKKQLSSVDLAITR
                     ATEYTVRAFLGKVYVYHSTMLNENKWQDAKAQLEAVKNSGEYSLVTNFRSLWSSKNEM
                     KDGNPESIFEASYTDIYGENWGGVATGNLDMQLMGIRDLQGYPGLDGGWGFCKPTQKL
                     VDAFIAESDSIRLDATVVAASWPASIAQWEADKKLLPSLNSKGAKYSEPYDPTGYWNG
                     KYRQDEHPLNADMYAQNELIYRFSDVLLLLAETEYHLGNEAAALGYVNQIRDRVHLAP
                     KSSTGMQLLNDIRKERQLELAMETQRYWDLIRWGLAAQEIPNFKTSTKGLWPIPQEQL
                     NVDPLLKQNEGY"
     gene            complement(33446..33847)
                     /gene="mce"
                     /locus_tag="PK252_07305"
     CDS             complement(33446..33847)
                     /gene="mce"
                     /locus_tag="PK252_07305"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOT14552.1"
                     /translation="MKPSHIEHIGIAVSNLDEAIKYYQDVLGLECYAVEEVKDQKVKT
                     AFFMVGQTKIELLESTDPEGPIGKFIEKKGAGIHHIAFAVDNVNEALTAVEQKGVTLI
                     DKQGRKGAEGLNIGFLHPKSTFGVLTELCSK"
     gene            34234..35997
                     /locus_tag="PK252_07310"
     CDS             34234..35997
                     /locus_tag="PK252_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HOT14553.1"
                     /translation="MKIKLAKSGLLVLCILAIIVAGCAKVGNIQGGPKDSIPPVVVKS
                     SPKNFSTNFKGKKIKIEFNEFIELKDLNKEFNMSPPVTKKPKIWLNGKVMVVEIRDTL
                     KDSTTYTLSFGNSIVDLNEGNKLSNFEFVISTGTYIDSMGVHGKIKDALTQKPLKEPL
                     LAMLYANLDDSAFFLQKPMYTGSVDKDGNFAVNNVKPGKYNLFVLSDLNSNLKYDIGE
                     MVAFCDSALVLSPEFITGKHKIEIEEDTARNDSVVMVVDSAKLRQARNSLHVNLISFI
                     EEGRKQYIKSYTRPNRRCVNIVFNKPLIKDSLSIDLINNKGENWYLKDRTNFKDSLFL
                     WIKDTTLIKTDTLRLGIGYYAFDKKDSLIYKKDTLLFRFIDTSNPKTKKPEEKANVLV
                     NAPQIFELNGIITIECPYPVLKIDKEKLILEEKVDTVFNPVAYDLIQDSIFQRLFVVN
                     NKWKEQTSYKMTILPGFANNIYGLPQDTIINSFTTKSLDSYGTLAINLTGPKMPVILQ
                     LLDGDKVKYEIKAETDGIIDFKYLEPKEYVLRLIEDKDNNGKYTSGNVLKKIQPENVI
                     YYGQMIKIRANWDMEINWELK"
     gene            36002..36922
                     /locus_tag="PK252_07315"
     CDS             36002..36922
                     /locus_tag="PK252_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT14554.1"
                     /translation="MEHHHPARKKHSHQRHSHAGDNLLIATVLNITITIVEVAGGLLS
                     NSIALLSDALHNFGDSIAVFLAYVSNKISQKGPSAKKTFGYKRVEIMAAMLNSLILVV
                     ICAYLLYESWHRMINPKPVQGLIMTIVATVGLLANLFAVFLLHKDKNRNINIKAAYLH
                     LLSDTVSSIGVVIGGLLIYFYKIYWIDPIITIIISLYIFKETWGILKQAYLILIQAAP
                     EGVEIDDILEVLERYEEIENVHHVHIWKLNDTEIHFECHIDMKNNVRLSETEELMHRI
                     KKILFEKFNIVHTTIQFEYNCCDDKSIIYK"
     gene            36919..37299
                     /locus_tag="PK252_07320"
     CDS             36919..37299
                     /locus_tag="PK252_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOT14555.1"
                     /translation="MKNYLTLAIILVLVISCGQRKTAVPASIIPEERMITILTDYHLA
                     QSLNTNYRVRNEMLKNMPVRLSDSVLKANGVTKAQLDSSLTYYATDLERFEKIYDEVL
                     NRLNKMQAKLQDTPKKDSSKVTKK"
     gene            37302..38471
                     /locus_tag="PK252_07325"
     CDS             37302..38471
                     /locus_tag="PK252_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT14556.1"
                     /translation="MRKVAANYVFPVSSAPIRNGIVTFNDEGIITEIKDNGGALMEAQ
                     GIEFYNGILVPGFVNCHCHLELSHLHKKISHGNGLMTFINELVSIRNVDIRDIKHCIS
                     VADSQMYSEGIVAVGDISNTSDSITAKMASKIKYHTFIELFDLYFKDVGELIANGALL
                     QKQYEELGLSSSIVPHAPYSVSPELFVNIASFHKKGSLYSIHNQETTDEDLMFLEGKG
                     VLFDMINKMPNSRFLPTGKASLPSYFDYLPNESKVLLVHNTFSRAEHIQLLKNKDITL
                     VLCPNANRYIENRLPDIPMFVKEGIKLALGTDSLASNYKLSILEEMKTIVASFPDISF
                     EEVLQWGTLNGAKALGLEADFGSFEVGKKPGINLICNFDFQKMTIDKDSFIKKLV"
     gene            38471..39271
                     /locus_tag="PK252_07330"
     CDS             38471..39271
                     /locus_tag="PK252_07330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT14557.1"
                     /translation="MIIESIQISDLSEIATLQPMDWPDIVPDIEYYIHSPFCRAVKVV
                     KNGVIAGIGANIFFEGTCWLAHIIVNESYRNKGIGTAIVANLMNYAKYEGYDTQMLIA
                     TELGEPVYKKSGFKKVTDYLFFKRTEPSHIETKSERIIPYSAQYKNALLELDRYVTGE
                     NRINILEKHLTSSYLIANQNEIYAFYIPSLGEGPIIAQDKEAGIELMQYKYATVDKAV
                     LPAENATGIEFLLANGFVQVSKCSRMLYGKEIPCHPEMIYSRIRGNVG"
     gene            39445..40137
                     /locus_tag="PK252_07335"
     CDS             39445..40137
                     /locus_tag="PK252_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14558.1"
                     /translation="MSFTHEVEGMICLAQGANHGPAPIPSEGKWVKAKEVKDISGLTH
                     GVGWCAPQQGACKLTLNVKDGIIQEALVETIGCTGMTHSAAMAAEILPNKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQTAFSEGGLPIGAGLEDLGKGLRGVTGTLYGTSAK
                     GPRYLEMAEGYITKIGIDKDNEIIGYEFVNLGRMMDMIKSGTDANEALKKASGKYGRM
                     EDAVKVIDPRKE"
     gene            40150..41157
                     /locus_tag="PK252_07340"
     CDS             40150..41157
                     /locus_tag="PK252_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOT14559.1"
                     /translation="MSQNAQLFEGYSRRINQINKVLNSYGISSLEEAKKICDDKGVDV
                     YKIVKDIQPIAFENAMWAYITGAAIAIKKGQKNAADIAATLGEGLQAFCIPGSVADDR
                     KVGLGHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANKVRKEPLRVILNGL
                     GKDAAKIIARINGFTYVQTQFDYATGEVKEVKRIAYSSGDRAKVNCYGADDVREGVAI
                     FHKEGVDVSITGNSTNPTRFQHPVAGTYKKECIEQGKKYFSVASGGGTGRTLHPDNMA
                     AGPASYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVALSQA
                     K"
     gene            41316..41678
                     /locus_tag="PK252_07345"
     CDS             41316..41678
                     /locus_tag="PK252_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT14560.1"
                     /translation="MKSENVVQQKSFDFAIRIVNVYKYLQTEKKEFVISKQLLRSGTS
                     IGANIEESIGGQSEKDFLSKVAIAYKEARETMYWLRLLLAAGYLTNEQSESLKLDAEE
                     ICRILGKIQITLKKKLHN"
     gene            41752..42192
                     /locus_tag="PK252_07350"
     CDS             41752..42192
                     /locus_tag="PK252_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOT14561.1"
                     /translation="MTKQETIDKAISFFEEGYACSQAVVLAFAPQFNIDETAAKLISS
                     TFGGGMGRLREKCGAVTGGFMVLGLAYGNTDPKDMDTKLAAYQKVRDINQLVIDKYGT
                     TMCSDILKQNVSENDVKQRNHHAIICYKVVGDVAGMVYDMLNKK"
     gene            complement(42236..42439)
                     /locus_tag="PK252_07355"
     CDS             complement(42236..42439)
                     /locus_tag="PK252_07355"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT14562.1"
                     /translation="MEKTKIGFDAGKIWQMLMEKGELKVTDLKKMSKMDIKDVYMALG
                     WLAREGKVHFFQLENEFAVCLSD"
     gene            complement(42470..42886)
                     /locus_tag="PK252_07360"
     CDS             complement(42470..42886)
                     /locus_tag="PK252_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOT14563.1"
                     /translation="MKKKEIVVTINDFAKLTGLIQTMRERKSIDVKYLDFLSYELGKA
                     KKIDSKKITPDFVTMNSIVKVCFADSDKTMDLRLVYPQQADFSKGMVSILSPLGCALI
                     GYKAGDTISFDAPKGKLFVKIESVVFQPEANGEDLY"
BASE COUNT        13523 a         7539 c         8702 g        13361 t
ORIGIN      
        1 caaaaaatca aattacgaca ctgatgtatt tacgccgatt atagaagaga ttagtcgttt
       61 aactggcata aaatatggca atgaaaatat gaccgatatt gctatgcgcg ttgttgctga
      121 tcacttaagg gcaatatctt ttgctattgc tgacggacag ttgccttcaa ataacaaagc
      181 cggctatgtt atccgtcgta tcttgcgccg tgctgtacgt tatgcatata gctacctcaa
      241 ccagcacgga ccttttattt gtaaattagt gccagccctg actgctaata tgggcaacgc
      301 tttccctgaa ttgaaagctc agcaaaagct aattgttcag gttattgctg aagaagaaac
      361 ggctttcctt cgcacattgg aaacaggtat taaattgtta gaccaactga tactgaaagc
      421 taaagctgca aaaacttctg taattgatgg taaatcggca tttgagcttt atgatactta
      481 tggtttccct cttgacctga cagagcttat ccttcgcgag caatcaatga gtgttaaccg
      541 cgaagaattt gaccaacaga tggaaatgca gaaatcacgt tcacgcaacg atgcagctgt
      601 cgaaacggat gattggcagg ttgtttcttc taaagataca actgagtttg ttggatacga
      661 cactattgaa gctgatgtaa aaatcgttcg ttacagaaaa gttactacaa aaggaaaaac
      721 acaatatcat ctggtgtttg ataaaactcc tttttatgcc gaaagtggtg gccaggtcgg
      781 tgatatgggc acaattacgg ctaatggtga agttgttcag ataattgata ccaaaaaaga
      841 aaacaacctg agtgtacata tctgtgaaca gctcccttct tttctttcgg ctacatttaa
      901 agcaaaggtg gacgaaacca aacgtttaga cacagcatca aaccactcgg caacacacct
      961 tatgcattat gcccttcgtc aggtgttggg caaacatgtc gaacaaaaag ggtcgttggt
     1021 tactcctgaa tatcttcgtt tcgacttttc acattttcaa aaactttcgc ctgaagaaat
     1081 agctaaagta gaagaaatgg tgaatcttca aataagaaag aatattgcat taaacgaaaa
     1141 cagggctatg tcatttgacg aggcacgaaa aaccggcgca atggctttgt ttggcgagaa
     1201 atatggtgac aaagtacggg ttatcaaatt tggggattct gttgagcttt gtggaggtac
     1261 ccacgttgct gctaccgggc agatagggag ttttaaaatt atttctgaaa ctgctattgc
     1321 tgctggtatc cgtcgtatag aagccattac cggtaatggg tttatgcaat atgtccataa
     1381 aaacgaagaa ctgataagct ctttaaaaga gatgctagga aatcctcaag atttgagaaa
     1441 ggccatggaa aagttgctta ccgattatag cggtttgcaa aggaaaatcg aacaagctca
     1501 aaagcaacag cttgatattg ctgttcaaca gttgcagaaa aaagttaaaa acataaatgg
     1561 tattgaggta attgccgaaa ctgttgaggc tgattcagcc caatcattga aagatattgc
     1621 tttcaggttg cgttctcatg ccgaaaatgc atatttagtg cttggtgctg taattgatga
     1681 taagccaacc cttgcaattg ccatatctga taaacttgta gcaacaaaaa aacttaatgc
     1741 tggccagatt atacgagaag ttgctaaatt aatcgatgga ggcggtggcg gacaaccatt
     1801 tcttgccact gcaggcggga aaaatgccgc aggattgtcc caggcaattg aaaaatcgct
     1861 tacttttata tagcaaaaca actctaacta ttcttaatat atactcaact aacgaactaa
     1921 ctaaattaac caatgaatgt aacacgtgtt tttgatttgt tcgaacacat taagaaggag
     1981 tacaacaagg cggatgtgtt tggttacaaa aaacaaggtg tatggcatac ttatcatata
     2041 gaccaatgta ttgaattggc aaatctgctc agtagtggcc ttctttcttt aggcttaaaa
     2101 aaaggagatc gtatcgtttc tatttcaaat aacaggccag aatggaattt tcttgatttc
     2161 ggcatgtcgc agattggagt gatacatgtt cccgtttatc ctactatcag caatgacgat
     2221 tttcattata ttttcaatca ttcagctcct aagtgcatct ttgtttctga acaatctgtt
     2281 tttaataaaa ttgaacctgt tgcaaaacaa tgtggcataa ctgatatcta ttcttttgat
     2341 gggctcaatg ggacagtaaa ttggaactat atccttgatt tggggcgggc acatattgat
     2401 gaacataagg ctgagattaa gctgttgaaa gatagtatcc ttccttctga tgtttttact
     2461 attatttata caagtgggac tacgggtttg tcaaaggggg ttatgctttc gcatgacaat
     2521 tttgttcaga atgtaaagaa tagcgtacat attcacgatg ttgaccatca ttgtcgtagt
     2581 ttgagctttt tgccattgag ccatgtgtac gaacgtatgc tcaattacca ctaccaatat
     2641 aaggggattg ggatatatta tgccgaaaat tttggcacta tagccgacaa cctgaaagaa
     2701 gttcaaccgc acatattttg tactgtaccc aggatacttg aactatttta cgaaaaaatt
     2761 gtcagcaaag ggtacgaatt aagctttgtt tcaaggcttc tatttttctg ggccattcat
     2821 tttggtaaga aatatgaagt aatgaagaaa aaatctttgt tgacaaagct taaaagaaaa
     2881 attgccgata aacttgttta tgaaaaaatc aggatgtcat tgggaggcaa cctgcgtgta
     2941 gtagtttctg gcggagcctc gctacaatcc agtcttgccc gtttattttg ggctgctggt
     3001 atccagcttt tagaagggta tggcctaacc gagacttctc ctgtaatttc agtaaacaga
     3061 ttgttgccaa aggtcgaaac atatccgggt acggtaggaa aagtgcttga tggtgtagaa
     3121 gtaaaaattg cccctgatgg cgagattttg tgccgtggac acaatataat gctcggatac
     3181 tacaagcaac cagagcttac agctgaagct attgataaag atgggtggtt ccatactgga
     3241 gatataggct tttttactcc tgaagggttt ttaaaaatca cagacagaaa aaaagaaata
     3301 tttaaacttt cgagcggaaa atatattgct ccccaagtta tagaaactaa attgaaagag
     3361 cttgaatata tcgaacaggc tattgttatt ggcgaaaacg aaaagtttgc cagcgctttg
     3421 gtggtcccaa atttttatca tcttcacaaa tggagcagcc aacatcatat tatttttcgc
     3481 gataatgaag aactaatcaa gaatcctgaa ataaataagc tttaccaaaa acttgttgcc
     3541 aatatcaatc ttcagcttgg tttggtagaa caaattaaaa ggtttcggtt ggtacaccag
     3601 gaatggacgc cgcaaaccgg cgaattatct gctactctta aattaaaaag acgcgttatt
     3661 cttgaaaaat atagtgatat catagaacag atttattctg cctcaaaaaa ttaaaattgc
     3721 tgttttttat aaaaatttga tataagtatg cttttttggt taaaatattt gtaaaaaaca
     3781 gtaaaaattg tttaattttt cgatttctta tgataattta aatcaaaaat tatattttta
     3841 cagcctttaa agttgaaaat tataaaaatt gattaatgaa actgtatatt cctaaaaatt
     3901 acaaaccagt tctgaatcta attcaaacag aaaaggcaat taagctggta aaagatttct
     3961 ttgaatcaaa cctttcaagt gaattgcgtt tacgcagggt aacggcacct ctttttgtat
     4021 taaaaggtac tggtatcaat gacgatttga acggggtaga acgcaaagtt acatttccaa
     4081 ttgccgatat gaaagagcag caagctgaag tagttaattc acttgcaaag tggaaacgta
     4141 tgatgcttgc agactacaat atccctcaag gttatggttt atacactgat atgaatgcca
     4201 tcagacctga tgaagagctt gataatattc attcattgta tgttgaccag tgggactggg
     4261 aacgtagtat gagcgctgaa gaacgcaatg ttgacttttt gaaatatata gtcagaaaaa
     4321 tttatagtgt actcaaacgc gtcgaatatg tggtgtatga agaatataat caaattaaac
     4381 caattcttcc tgaagaaatt acattcatcc atagcgaaga gttgctgaaa atgtacctca
     4441 atctttctgc taaagaacgc gaaacggagt ttgctaaaaa acatggagct atattcatta
     4501 ttggtattgg cgataaactt tctaatggag agcctcatga tggaagagct ccagattatg
     4561 acgactggac aaccaagtct gaaaacggct ttaatggttt aaacggagat attatccttt
     4621 ggaaccctgt tttagaacaa gcgtttgaag tatcttcaat gggtataagg gttaataaag
     4681 aagctttgtt aaaacagctt acaattgcta aagctgaaga ccgcaaagaa ctgatgttcc
     4741 acaaacgctt gttaaatgac gagttgcctt tgtctatagg cggaggaata ggacaatcac
     4801 gtatttgcat gtttttcctt cgcaaagccc atattggtga aattcaatca agtatttggc
     4861 caaaagaaat ggttgatgaa tgtaaaaaaa atcaaattat tctgatctag ttatttgaaa
     4921 aaataagagt aagagagata gggcttttta gctttatctc tctttttttt acctttgtct
     4981 tccaaaaata aaatcaaaaa aatgacgttg attaagtcta tctcaggaat tagaggtact
     5041 ataggcggta agccgggaga taacctcact ccactcgata ttgttaagtt ttcagccgct
     5101 ttcggaacct ggcttataca acaaaataaa caggctaagt gcacggttgt tgttggtcgt
     5161 gatgcccgta tttctggtcc tatgatggat cacattattt gtggtacatt agtgagcctt
     5221 ggtattgatg tgataaacat tggtttggct gccacgccta ctgtagagat tgcagttaca
     5281 gaaactaaat caaatggagg tattatcatt actgcaagtc acaatcctaa acagtggaat
     5341 gcattgaaat tgctcaatga aaaaggtgag tttattaatg atacccaagg tagattggtt
     5401 ttgcagttgg ccgaaactga acaattcaat tttgttgatg tagataagct cggcaaggtt
     5461 tcttatcgcc acgactttaa tgatattcat atacaaaaaa ttcttgagct gcctttggtg
     5521 gacaaagatg ctattgctaa agccggattt aaagtggcaa ttgactgtgt aaactctgtt
     5581 ggaggagttg tgttacctca gttgcttaaa gcacttggag taactgaagt gttgcctttg
     5641 tattgcaatc ctgatgggca gtttcctcac aatcctgagc ctctgccaga acatttaaca
     5701 gaaatttcag cactggttaa ggataaaaag gccaatcttg gttttgttgt tgatcctgat
     5761 gttgaccgcc ttgctattat taatgaagat ggttcaatgt ttggcgagga atattcgctt
     5821 gttgctgttt cggaatatat tttgtctaaa actcccggaa atactgtttc aaacttgtcc
     5881 agtacacagg ctttgaagga tgtgactgtt aagtttggag gtacctatac tgcatcagca
     5941 gtaggagagg taaatgtggt tacagccatg aagcaaacca atgctgtgat tggtggcgaa
     6001 ggcaatggtg gtgttattta tcccgaattg cactatggtc gcgatgcatt ggttggtata
     6061 gcactatttt tgacctattt ggcctacaaa aagatgagct gtagccagct tcgcagtact
     6121 tatccatctt atgtcatttc aaaaaataaa attgaattga agccaggcat tgatgtagat
     6181 gctattttgg ctaatataaa agataaatat aagagtaaac ctataattga cgtggatggc
     6241 gttcgtatta actttgaaaa tcaatgggta catctccgta aatcaaatac agagcctatt
     6301 atacgtattt atgctgaaag taagactatg gacgatgcta ataatttggc ccagagtatt
     6361 atgaacgata ttaaagctat gtcgtaaaag gcttttgaat aatcaggtat gaatgacttt
     6421 gagcgggtta ctattttaaa ataatcacca taactctgcg ttgttggttt attataatgc
     6481 cagttaagca attagctggc attctttttg agctcttcaa agattcttaa ggcagacaaa
     6541 aagtctgtaa ttttgtatgc gtgtattcct gttatagtac cgtttatggc ttcgttaaga
     6601 aacgattcag ctttatattc aatattttcg gttatagtac tgcccgtttt gtagtaaata
     6661 aggttaatgg cgttgtccgt ccattccaaa gagcctgttt tttcttcgtc acaaattttg
     6721 atttgaaagt taaagctgtc tttgttattg gtatttattt cgatatgaac agaattgcca
     6781 ttgataaaag agatataggt accattaatg ctcatttctt tgttgttatg gcgtgacgaa
     6841 tatgaatagc atttttttat ttttccatgc atcaatgcgg ctactatatc gcaaatggta
     6901 gtatagtgtt tattatactc atgcttttcc caaaaactta tgtttgtgta aatctcaatt
     6961 ttaaggtatg aggtattttt aaaaagcttt gataaagcaa tagcttttgg gcttatatga
     7021 ggttggatca ttatctcggc ctcaatggat agcatctgga gatactttat ttgctcatgc
     7081 cttaaaaaat atggatttaa caaaatgata tgctttgact gctgaatact ttctatggcc
     7141 aggttatacc attcgggctg cgatacatca aaaattagga tgtctgaatt ggaaataagc
     7201 agaaaaggat tggtactatt ggcttgttca ggagcattgc ttgacaggtc gatgcaaatg
     7261 gctgaatggt gtatattttt tgcattcagg aagctgttta taagagtttc attggtgtca
     7321 ggtgtacaaa ataagccaat cttgctcata ctctatcctt tataatgcat acaaacttaa
     7381 ttcataataa gtacattatg cagatgtgca taaaagagtt tatcaactaa ttgttaataa
     7441 aaattgtttt gcaaaaatcc tgttggtata gttgtttacg tgctgttaaa tcaaatagtc
     7501 agcgtatttt catatcgaaa aagtttcggt taaatcttat caaaaggtat aactttgtgt
     7561 agcattttta gagggataag aaattttcat ggaagataca ttcagacata aaggtttgcg
     7621 aaataaattg gtggaatgtt tacgcctgaa aggtattcgt gatgagaagg ttttactggc
     7681 tatcaataaa gtacctcgcc atttgtttct cgaaagtagt ctgataaatc atgcatatca
     7741 ggacaaggca ttacctattg gggccgggca gaccatttca cagccatata cagtagctgt
     7801 acaatccgag cttttgaaag ttaaaccttt cgaaaaggta cttgaaattg gtacaggaag
     7861 cggttatcaa gcttgtgtcc ttctcgaaat gtctgcaagg gtttatacta ttgaacgtca
     7921 gcgagagttg tatctcaaag ccatgtcatt tctgcctcag attggttata acccgcagtt
     7981 tttttatggc gatggctatt taggattgcc tacttatggg ccttttgata agattattat
     8041 tactgccgga gctcctgaag ttccaaaagc cttgttagaa caattaaaga ttggaggtcg
     8101 tcttgttgcg cctatcggag acagccaggt tcaggtgatg acggtggttg aaagaaaagg
     8161 cgaagattct tttgttacaa ctcatcatgg aagttttatc tttgtacctc ttttgaaagg
     8221 aaccgaataa atgaattcat attacaccct tacatataat ccttttggtg taaataccta
     8281 cgttatatgc aatgaacaga acgaatgcct gattgttgat gccgcttgtt atgataaacg
     8341 cgaattagag catttataca actttataga gggtaagaaa ttgaaaatag ttgcagctgt
     8401 taatactcat gggcattttg accatctttt cggggttgat gaggtttgca gcaagtacaa
     8461 ccttacccca tacattcatc aagatgatat gtatgtggtt aaagcagcgg cagaactggc
     8521 tataccattt ggcttagttg tcaggagcct gcccccgaag tggaactatt ttaccgaaga
     8581 cggcatggta tctattggtg gttttaattt tcgggtaatg caggttccgg gtcattcaaa
     8641 aggcagtgtt gtcctgtatt ttgagaaata taaaattctg gtgacaggag atgtgctttt
     8701 tgcaggaagc ataggccgta cagatttgcc gggaggaagt tatatagacc tgatttcggg
     8761 tataaaatct aagattctta atttgccttt agatactgtt gttttgccgg gtcatggccc
     8821 atcaactact attggtgaag aaatttcgag caattcattt ttaaattatt tataagatat
     8881 aaaaatgtca caggagaaat ttttatacag gcatattggc ccgagcgact cagaagttcc
     8941 ggcaatgctt gaaactattg gggtcgaatc tgttgatgat ttgattgcca agaccctgcc
     9001 tgagaatatc ttgctcaaaa gggtcttaag gcttcccaaa tcccagactg agcatgagta
     9061 cctcaagcat atttgggaga ttgctcagaa aaaccttgtg tataagtctt acatcggttt
     9121 aggatattac aataccataa caccgtcggt tatacttcgc aatatattcg aaaacccatc
     9181 atggtacact tcatatactc cataccaggc tgagatttcg cagggtcgtc ttgaagctct
     9241 gctcaatttt cagacggttg taatggagct aacaggtatg cccattgcca atgcttcgtt
     9301 gctggatgag gcaacagctg ctgccgaggc tatgatgatg ttttacaatt cacgtacttc
     9361 tgcgcaagct gaaaataggg tactcaaatt ttttgtggac aaaaatagct ggccacagac
     9421 catagatgtg ttgcgtaccc gtgctttacc ttttgaaata gaggttatag ttggagatta
     9481 caaaacatac tcattgggta atgacttttt tggggcatta gtgcaatacc cgggtgctga
     9541 tggtatcatc catgattatt ctggttttat tcaaaaagct cacgaaaagg aaattcaggt
     9601 ggctgtagct gctgatatat tgagccttac cattcttgta cctcccggaa atattggggt
     9661 agatgctgta ttgggttcaa cccagcgttt tggtattcct atggggtatg gaggaccaca
     9721 tgctgcttat tttgctgtta ctgagaaata taaacgtgcc attccgggcc gtattattgg
     9781 tgttacaaaa gatgtacatg gcttgcctgc tttgcgttta gctattcaaa cccgtgaaca
     9841 acatataaaa cgagaaaggg ctacttctaa catttgtaca gcacaagctt tacttgctat
     9901 tatggcaggt atgtatgctg tatatcatgg aaaaactggt ttacaacata tcgcattaca
     9961 cattcaccgc tcggcttcta cattggctca caagattgaa gccctgggtt atgaagtggt
    10021 tagcaaaaat attttcgata ccattgaaat caggctgcct catgaggtta cctctgatga
    10081 gttacagcgg atagctgaag aacgtatgat taacctgcga tacgaagcta aaaatactgt
    10141 atttattagt gttgatgaaa ctacaacaat agatgacctt aatgttttgc ttgatttatt
    10201 tgctgtcgct attttcaaaa agtatcaacc aatagagagt cttgagcata gcttgtcttt
    10261 tgataatatg catttgcgtg acgatgatta tttcacacaa gatatattta aattatatag
    10321 ttgtgaaaca gagatgatgc gctatattaa aaagcttgaa agacgtgata tttctcttac
    10381 acaaagcatg atttcgttgg gctcatgtac catgaagctc aatgctgcta cttaaatgct
    10441 ccctcttagc tggcctgagt ttcagtgcat acatccattt gctccgctcg accaagtgga
    10501 aggatatcat cagttgctca atcgacttgg caaatggctt tgcgagatta ccgggatgag
    10561 ccaaatcacc tttcagccaa actctggtgc tgctggagaa tacacgggtt tgtatattat
    10621 tcacaagtat cacgaaagca aaaatgagca tcaacgcaag attgtgctta tcccgtcttc
    10681 tgcacatggt accaatcctg caagtgcagc aatggcaggg ctgaaagtcg ttgtaattaa
    10741 ttgcgatgac aatggtaaca ttgacttcaa tgacctgaaa gaaaaagcaa atcaatacaa
    10801 agaagaactt tcatgcctta tgttgacata tccatcaacc catggagtgt ttgaagagca
    10861 ggtgaaagat attattgact atgttcattc attaggagcc caggtatata tggacggggc
    10921 caatatgaac gcacaggttg gccttaccag tccaggcaca attggggttg atgtttgtca
    10981 cctcaatttg cacaagacat ttgctatacc tcatggtggt ggggggcccg gaagtggtcc
    11041 tgtttgcgta gctgaacact tagttgagtt tttgccaacg catccgcttg ctgctgtggg
    11101 ggggcaaaaa ggtggtactg ctgtatctgc ttctccctgg ggtagtgcgt tgatagcaga
    11161 aatttcttat gcctatattg ctatgcttgg tgctgaaggt cttgctgagg ctactaaata
    11221 tgccattttc aatgccaact atatggctgc aaggctaaag aaaaagttta aaatacttta
    11281 tacgggcaaa aacggctttg tagcccacga gatgattatc gattgtcgtg aatttaagaa
    11341 aatggctgat gtttctgaaa ttgatatagc caaacgcctt atggattacg gttttcatgc
    11401 tcctactgtt tcttttcccg ttcatggtac cttgatggtt gaaccaacag aaagcgagtc
    11461 aaagcaagag ctcgacagat ttatagatgc tatgtttgcc atttatgaag agattgaaga
    11521 aattgcttca gataaatatc caaaaaatga caacgtgttg aagaatgctc ctcatactgc
    11581 tgctcatgtt gcttccgata gttgggcgca tccttatagc aggcaaaaag ctgctttccc
    11641 attatcttgg attcaggaga ataagttttg gccagcttgt ggcaggacgg atgatgcttt
    11701 tggcgaccga aacctggttt gctcgtgttc gcctattgat gagtatcgga agagcttata
    11761 aaaattgagg ttatataaaa tgtaaaatgt ctgaacattt aacaagtttt gttcagacat
    11821 ttctttttat ttaattattt ttatactttt ctcaaattcc agggttactt ttttgcatct
    11881 tcggacatta atagttgtat atataaatgg agcagttttc aaatgaggat attatcggag
    11941 ggattttaaa taataacagt aaggttcttc ggtatattta tgctgaaaat tttccgaaag
    12001 taaggcaaca tatatgcata aatggtggca gcgaagatga agcagcagac gtttttcaag
    12061 aggcaattct tattattttt gaaaaggcca ggaaagagca gttagtgctg acagtcaagt
    12121 tttccactta tttatacgca gtggccaagg ttgtttggaa gtcctattta agggaaaaca
    12181 gaaaagaaga agacatgttt caattgaatg agactgaatg ggattttgaa attggagaag
    12241 ttgacgatta tattattgat aatgagcgat ttaaacttgt ttggagatat tttgacaata
    12301 ttagtaaaga ttgtcaaaca ataatacggt tgtttttaga aggaaaaagc ataagcgagg
    12361 ttactcagga aatgaattac agcagcgaac agcatacaaa aaaccgaaga ttgcgatgca
    12421 aagagagttt gataaaaaag ataatggctg accctaaata taaagagctt tgtagagaaa
    12481 taaaaataga caaaataaat agatggtaaa taaagataat attgcctgtt tttatactat
    12541 aggaattgct tgcagggcgt ttgttttgat tgaaatgtaa ttgtctaact gataatgttt
    12601 tcaggtatta tttaatagat atttttcata tgcaaaaatt tttttaaaat gaaacaccta
    12661 tgatgcaaaa aattgcaaac acgttgatgt ttattgtggg tgcatactat catttcatta
    12721 aaaattaaaa aataaatatg atgaaatttg agaatgaaaa tgacctgaag ttgtattcag
    12781 attatgaaca tgatgtggat acatttcttt cagatgtaga aaagaaaaga ttgattgata
    12841 accttcaaac aatacaggtt gaatatattg aaagtcaaca tgatgataag aagacgccta
    12901 ttcgtctttc aagaaagaag tattattttg ttgcagttgc catgttgatt ttgttgattg
    12961 gagttgcagg tgtgttaaaa ttcagcattt taaaaaccac aatagagggg acagatattt
    13021 ttttacaata ttattcggtt tacaaagttg atggtgttat gcgttcagat atcaatgcaa
    13081 actccgagct ttcattagcg cttgaagctt attctgaagg caattttgga aaggcctcgt
    13141 cattgttgaa aagtcatgcg tattcaggaa tcgagtttaa taaggctcag ttttattttg
    13201 gtttggcaag tatagaactg gcagattatc aggttgccat tgaagctttc aacaaggtgt
    13261 tgagcaatgg taataatcca tacattgctc aatcgcattg gtatttggct ttaacatggt
    13321 taaagctgaa taattacaat gcagctaaag aacatttgca atggcttcag cagaacgaca
    13381 ggttttacgg gaaaaaagca aaggagatat ttaataaatt gaatgacaat taataatcta
    13441 taaataaaat ctattggcca taatattcat tagttttaca ttgacagatt agttgttcat
    13501 tgctgttaga cgcttagcgg cgaaacccat gtttattagt tttcatttta ttaaatatta
    13561 aggtaggatt aggttatttt ataaaacgtc agtatgtcac aagaagagtg cttaggtata
    13621 tgatatttac tggcattgta aacttttagc ataggagcaa aacttacaaa attcaaggta
    13681 gccttaaata ggctaaaaaa gctttatcat ctaaggtaca gggcaataac ttgctttttt
    13741 caaacagttg cttttacatg gccgataagg cttttttctt aaggtcattt acgtcaggca
    13801 aacggcgggg cctttaccag aaaaatccct tttcattgct ttataacaat aattaacgat
    13861 gttattctta tatcgacaca tgttaagtat taaaaggtta ctaaattttt tgaaccatag
    13921 ttgtgttttt ttgtttaaac attaagcatt aacaagtact ttaaagtaat gacatttttt
    13981 taaaacattg ataatctttt tattgtaaat atgtttgata ttttattatt tttgtagcat
    14041 caaaaaaata ttttatgcgc tctgagattg attatttcaa aatagaagcc aatcacgttg
    14101 cgcctgaaca aggcaatatt ctcattgctg agccattttt agcagataat tattttaaaa
    14161 ggtcaatagt ttatcttgtc gggcatggag tagaagggtc tgttgggttt attctcaata
    14221 agccggttag catgaagatt gttgacctta tccccgattt tccatctaat gatttttcgc
    14281 tttctttagg tgggccggta agccccaaca ctattcatta tatacatacg cttggcgata
    14341 ttattccaga cagtgttcgg gttaaagatg atatttattg gagcggagat tttgatgttg
    14401 ttaaacaact tatcgctgct aagcaaatta acaagtcgca gatacggttc tttattggat
    14461 acagtggttg gagccccgga cagctcgaaa atgaaattga tcaaaattca tggctaatcc
    14521 atcattcaaa caacaatttt ataatgaaga atgataaaga gcatgcctgg tcaaagattc
    14581 tttatgatat gggcggaaaa tatatcatgt gggccaattc gcctgaaaat ccttcgatga
    14641 actaaagtta aaaataaaaa ggtaaaagaa ataggatatt ttattgcctt tattgtcttt
    14701 actcttttgc cttctcgctt ttacctttct ttctactttt atcacactcc aaaaacactc
    14761 ttgttgagca gctttataag attctggctc atggcattgt agtacctgcg gtttcggaaa
    14821 gcttgcaccc agcgaatccc gcttattgcg ttggtcaaaa atatttcatc tgcttcaagc
    14881 aggatgcttt ctgtgatagg aatgttgtca atgacattaa taccagcctc tttagcaata
    14941 gtcattaagt aacgcctcat tactcctgac acacaaccag agtcagctgc aggggtcagt
    15001 aatacattgt tttttactac aaaaagattg gaattgcagc tttcaataag ctcttgtttt
    15061 tggttcaaaa tcagacaatc gtcataaccg ttttcatggg caaaaatgct tgccattacg
    15121 taaatagctg aagatgtggt tttgaaagaa gagatactgc ttgcagtttt agtgatatct
    15181 ttaaaaattt caatactgca accttttttg ttgagcagat aatatggctg ttccagttgt
    15241 tttgtttcga taatatacga aaaatcatta gtttcgggaa gaaaccggcc tccatctttt
    15301 ctgaatagaa taactttcac tcttgcacca gtaaatagct tatttttatt gagcagatgc
    15361 gttatttgtt ttgaaatagc tgctggtgta aatgatttgt cgggttgcat ttttaaagcc
    15421 tttattccat tagtaagacg ctcaaagtgc aaattaaaaa gctgaacatc tgtgccataa
    15481 gcatgcattg actcaaacaa accatcccca aagcagaatg aacggttgtt gtggttaaac
    15541 aattgctcat cagacctgta aatattgcca tcaacaatga tgaaagtatc atttgctgcc
    15601 atattcttta attatgctat ccacaaacgc tgctatgtct gtattttttg ggacaagaat
    15661 accttttatg ttggctttgt cagcggcttc aacatcccgc ttgctgtcac ctataaaaaa
    15721 ggccatgttt ttgtcaatat tgaagcgggc tattgctttt tccaacaata aagagtctgg
    15781 cttacggcac aaacaccttc cagattgctc atggtgaggg caaaagtaaa gctcagtaac
    15841 agttccgcct ttttctttta ccttttcgca aaggattttg tgcaattctt cggtgtgggc
    15901 aatggtgtat aagcctttgc caatcccgcc ctggtttgat atgattatca ccagaaagcc
    15961 tgctttacag agctttgtaa tattgctttc aatattttcg tttaaacgaa agtcatttac
    16021 attgtatatg taatacaacc cttcatcatt gttgattacc ccgtcacgat ctatgaaaac
    16081 tgctttgttc attgtattct gttaaaaact aaaattaaaa acctgttcaa taaagccata
    16141 aagaagtttt ggttctaata atattgggaa agtgaagcca tataaggccc ctaaaaggtg
    16201 agcatcatga ttgatattgt catgattgcg tttgctcata tattgtgaat aaatgaggta
    16261 taaaaccccg aaaataatac caggaatagg tattatgccc aaaaaaagga gtttttgcca
    16321 gggattgaaa aaaatacacg cgaaaactat agtggacaca gccccggaag ctccaacgga
    16381 gttgtaaatg aaggtgtctt tgtatttttt gagtgttgta agcgaagcta ccacgatagc
    16441 agtgaaatac atggtcatgt aatatgcaac gacaggaaat ctaaggctta ccattccctt
    16501 taatatagaa aggtaatatt ctaagcctag tccaaaagaa tagaggacaa tcatattgaa
    16561 aataaggtgc atccaatcgg catggataaa agcatgtgtt atgagccgcc agtattgctt
    16621 gcgatgaact actttgtagg ggttaagttc aaacaattcg aacaattgct ggttataaaa
    16681 cgctattgtc gaaataatag atgtagcaac aactaaaaat actgtcatgc tttttttgtg
    16741 caaaaataaa gaaaaaaggc atagtaaaaa gaggcaagaa ataacagaca agagttaaat
    16801 ggtgtcaaaa tgagttttgc tcattttttg tttcttataa aagccctaca tttgcattac
    16861 aaatattcaa aacctcattt tgtggaaaac aggctgatgc catacaaaga aaaacaagaa
    16921 caatttgaca ggcgatacct tgaaatggcc cggatatggg ctaaaaattc gtattgcaaa
    16981 cgcagacagg taggcgctct cattgtaaaa gacaaaatga ttatttctga cggatacaat
    17041 ggcactccat cagggtttga gaataattgt gaggacgaga gcgggcgtac atatccttat
    17101 gtgctacatg ccgaggctaa tgccattacc aaagtagcaa aatcaaacaa tagcagtgaa
    17161 aatgctactt tatatataac cacttctcct tgtcttgagt gtgcaaagct tatcatccaa
    17221 tcgggtatta taagggttgt ttttgctgac aaatatcacc aggaagatgg tataaacctt
    17281 ttaaaaaaag caggtataga aattgtacat ataaaatttg aataatatga gtgttgaaaa
    17341 tatgaataga agaaaaatat acctaccgtt aattatttct gtgtctattg tagtggggat
    17401 attgttgggc tcatttctga caaaccgtat tcaaagcgga agaaacagca ctttgatgtt
    17461 cccatcatcc gggaagctca ataatatatt gcaatatatt gaagaggaat atgttgatac
    17521 tgtgtcctct gaccgattga ccgagtcagc tattgtgtca atgctaaaag agcttgatcc
    17581 acactcggct tatatcccag catctgaatt gcaagctgtt actgaccctc ttgaagggaa
    17641 cttcagtgga atcggggtgc agttcaatat gcaaaacgac acagttgtaa ttatgaacac
    17701 tgtaccaaat gggccatcgg ctttattagg aatacaggct ggagatcgca tagtgaaggt
    17761 taatgatacg gtagtggctg gtgttaagat ggaaagcgat gctatagtcc gcaaactcaa
    17821 aggcccacgt gggacaaaag ttcaggtaag catcttgcgt ccaggcaata aaaagcctat
    17881 tttgtatgaa attacccgtg acattatccc gttgaacagt gttgacgtgg cgtacatggt
    17941 tactcccaaa acaggatata tcaaaataaa ccaatttgcc cgtactacgc ctgaagaatt
    18001 tgttaaggca gtgacaaatc ttagaaattt ggggatgcag aaccttgttg ttgatttacg
    18061 cggcaatggt ggcggatatt tggatgcggc tattaatctg gcagaccagt ttttgcctga
    18121 aggaaggctt attgtatata ccaaaggcag ggccaaagcc cgtgagaact tcacagctac
    18181 ctattcggga gtatgcgaag gcaatgaaat agcaatatta atagacgaat tgtctgcttc
    18241 ggcatctgaa atccttgccg gggctattca ggataatgac agggggacta tcatcgggcg
    18301 ccgctcgttt ggaaaaggct tggtacaaga gcaaatacca ctttcagacg gctctgcatt
    18361 aagattgacg gtagcaaggt attacactcc ctcgggaagg tgtatacaga agccttatca
    18421 caaaggagat gaagattatt ttctcgacat tactaagcgt tatcagcatg gtgagtttga
    18481 aaataaagat tccataaagt taaacgatag tcttaaatat acaacgctta aaggacgagt
    18541 tgtttatggt ggaggtggaa taatgccaga catttttgtg ccattagata cggccggttt
    18601 ctcaaggttt tatgaacaga tacgaagcaa tgggttgatt tatcggtttt cttttaaata
    18661 cagtgataac aaccgtcaac gccttaaatc tatagctaat cttgatgcat taagaaaata
    18721 tcttcgtgaa gacaaagttt tcatgaagtt tattgatttt gcctcagagc agggagtaaa
    18781 agcaagtacc cgggatattg aagtgtctaa aaagtacatt tcaacagagc ttgaggcttt
    18841 tattgcccgc tatttctttg atgatgaagg tttctaccct atcatagggg aaatagacaa
    18901 tacattgtca aaagcaatag aagtgctgga gaagaagtaa ttagcaaatt agcaaattag
    18961 caaattagca aattagcaaa ttagcaaatt atttttctta gatataatct tcgatttcaa
    19021 atcattattg tcgggttata ctgttttatt ttgatattcc tcactacgtt cggaatgaca
    19081 atcagttatt taagggttaa aggaggggct ggtttgcggc tttgctacaa accagccctt
    19141 ccttcatgtt attttcaaaa gctttgttat tcagacagag cgaagagata aatcttttca
    19201 ttctcaaatt ttcaaatttt caaattttca aattttcaaa ttttctcatt ggccaattat
    19261 ttattaaccc atatccgaaa atcgtgaagg ttgtttacgc ttacaatggt ttcgaaatcg
    19321 gctttgggtg aaagggtcag cttaagcctg ctttttgaaa ctacttccat gctttcgatt
    19381 gcgtcaatat gcacaatgca ttgcctgtta atgcggaaaa aatgttttgg gtctaatact
    19441 tcttcaagct tttcaagtgt ttgcgagaca agatagtgcc gattttcttt tgtgacaaag
    19501 aaagttccgt tttcgagtat gtaaaaataa gctatatttt cggttttgat agatttgagc
    19561 tttgtacctg ccgaaaccag gaagcgttcc tgatatgaag gccctttttt caagctttca
    19621 aaaagtacag agaaatctgg cgctggagat tctgcagccc gcgtcttata cttttgtata
    19681 gcctgcctta agtcttcaat attgataggt tttaataaat agtcgatgct gtttacctta
    19741 aaagccttta tagcgtaatg gtcgtaggct gtagtgaata taattggcgt ttcgactttg
    19801 atttgttcaa atatctcaaa ggatatgcca tccgaaagct gtacatcaag gaaaatcagg
    19861 tcacaactgt tttttgaaag ccagcttaca gctttcttaa ccgaatcaag gtgagccaat
    19921 atagatatgt tgggatctat gtcttgtatc agcgattgaa ggtaatctgc tgcaggtttt
    19981 tcgtcttcaa taacaattac gttcattgtt cgtcttttat taaagggatt tttgcaatat
    20041 attgtttttc agtagtataa aattctggca tagcgtcaca tagctggtta taccttgctt
    20101 gcaggttttt caaccctata ccagttgact cgggccttat cctcttcatt tgaagattgt
    20161 tcatcaccac aatatttgaa ttttctacaa aaatcttgac ctgaagcggg gcttcttgcg
    20221 atgcttcatt atgtttgaag gcattttcga caagcaattg caatgaaagc gggggaataa
    20281 agtcgttcat gtattcactc tcaatttgta tatcaatgtt tatggcatct ccaaaacgta
    20341 tcttttgtaa aaagatataa gaattgataa agtccagctc ttgtcttacg gtaataagca
    20401 ttttgtcgga tgagtcaagc acgtaccgat atattttcga gaatttgtca acaaaaccaa
    20461 tggctttatc tggtgaagtc cttattaaag aactaagagt attaagactg ttaaacaaaa
    20521 agtgcggatt tacctggctt ttcaaggcaa tgtattgcga ttcggctttc tcccgttgaa
    20581 gacgttcagt aaggatcaac gtctctttcc atcttctaaa aatgatataa gcttcagcaa
    20641 gaccgcaagc tattgcgttt acaattaatg caacaataat attttcaaaa aataaagcgg
    20701 caactttttc attctggcct ttggggaatt ctcctaagac aatatagaac aggtaaacaa
    20761 tgatggaaat gattacagcg gcattaacag aggttataaa catttctacc aaaaatatgc
    20821 gccaataacc tttatgcccg gcatatttat tttcgaaaaa gcggaaggta aacaggttag
    20881 ctgatgtgat agacaaggtt acaacaaaag taaatgttat atttaaaaga atttcattga
    20941 cgacaatttc cgtgttgttg agccaattac ttatcagata aactgaaggg ataatgattg
    21001 ttgtgcaaat gcaaacgaga gctatcgctt ttattattac tttccgcgta agatgcatgt
    21061 tttgctattt tagaaattaa ttctccacat catcattgga aatatgccca gttggtttac
    21121 ttttttcaac cttaatgtat tagaatcaaa gtattcgtat agcaggttct gatgattggt
    21181 tacattttct acaaatatct gccattcctg cgaatatctc aaattattgt ttttgatagt
    21241 aaacttcaca tccagcttaa agaacatggg gtattgtttt tcaaaagcct ggtcttcttt
    21301 atatttagta tctctttcct gaagggactt tgcaatgtca acctcggtat atcttttgcc
    21361 tccggcaaaa gacgatttga tgtcaaagca aagcgtccat ttttggctta cagggtgttc
    21421 atagcctacc aatgcattga ctacatagtt gccattgaat gccgtattgc gccatttgtt
    21481 gtcactggct ttgtatttcg aatcaaacaa ggatgtagtt acaagaaaat aatagttacg
    21541 gctcaggaac ttttcaaaag taagttcaaa tccatagttg tagccttttc ccttgtttac
    21601 aagactgtct tctgcattca cgccccagct tgcaccgcta ttgagaaggc tgaagctcga
    21661 gctttttgtt tcaacaggga cattaaacag gtactgataa taggcttctg tttttaaacg
    21721 gaaatcttta tttatgttcc aatcgtggcc gataacaaag tggtgagctt ttgtaaaatc
    21781 aaggttttta ttggtttcag cacgggtagg ggcatcagct tcaaccaaat aatacactga
    21841 taacgggtga gttttagaat gcaagccata gccaatgttg atttttttgc cattgctata
    21901 ttcaaaaatg gatgcagccc tcggttctaa agcttttgaa ttgttcaaag aaaataaggt
    21961 agcatataag ccgggattaa aagtgatttt gtcagtaatt ttaatactag cctcgtaaaa
    22021 agctttatat aactgaggcc cttcaaataa tgattttgag gcatccattg cctggtaaag
    22081 ccctggttgt ggtacattct ttgttagata tatccttgtg tccaggttgt agcccatctg
    22141 gtctatactt ataccaagtt ttgtattgaa cttagcattg tacttattgg caagagaggc
    22201 tgtagctgta gtcctgtatt cagaaatgta atgttcaaga gtacgcgttg gaattcggta
    22261 ataataaaaa gtatcagtgt ttacaatgtt cttaaattgt tgtaaagtgt caatatttgt
    22321 gccgccttcc tgtgcgaggc ccgaaaggga gattttaagt acttttcggg tatcggggaa
    22381 caacgtgaga gaaatgcctg aagctgccat cgaagagtaa ttcgttaaat cctggcctga
    22441 ggaagtgtac atatcgtctt tggcttcatc ttgcttgctg tcgaacattt ctatgctact
    22501 tttaccagct ataccaaaac aagttattac gccattttta atcgggtaat tggctttgaa
    22561 ggataaatcc tggtattttg ggactcctga ggtgccaaaa gagaagccca ttttgtacat
    22621 catatccaac gttgagtaac ggtaattgat taaaaaagag cttttgtttt tcttactaat
    22681 aggaccttcg gcacccgctt caaacccatt gaaaccaact tgtccaagga attcgtattt
    22741 gtcagtattg ccgttgcgca tcttcaggtc aaatactcca gagagcgcat taccgtattc
    22801 ggcggcaaaa gccccggtca tgaagtcaga atttttcagc acgttgttat tgagcatccc
    22861 caccgggcct ccggtagtcc cgttctcggc aaagtggttg ggattgggaa tttctgtgtc
    22921 ttcaagtttc caaagcaggc ctgacggtga gtttcctcgg atgataatat cgttgcggct
    22981 gtcattggca aaagaaacgc ctgcaaagtt agatgccatg cgagctacat caccacgact
    23041 gcctgcgtac cgctctgttt cttctacagt aaacatgcgc gagcttacaa ctgccatttc
    23101 gttgtttgcc ctggctttgc ttttggcagc agtaacaata acctcgtctg ttaccgttac
    23161 tttttcttcc atttcggcat taatcacaag ctctttgccg ctaataaccg ataaacctga
    23221 gaaggtggcc tggttgtagc caaggtagga gatagaaagt gttacccttc ctactggtat
    23281 gtctgttact ttaaaataac cttcttcatc ggccattacg ccggtttctc ctcctgtaaa
    23341 ttcaaccttt acagatgctc cgggaaggcc ctggcctgta actttgtcaa tggcccgccc
    23401 tttgatagtc tgtactattg ctggttttaa attttgggca gaaatggtaa tgcttgcaac
    23461 aaaaaatgta atgcataaaa gaatgttttt caaatttgtc atgttttgtt tgtttttggt
    23521 tttcgttatg acaaacaaaa ttatcccacc ctctactgag gttaaaaaac ttttttttga
    23581 acaggggaaa aaatatggtg aacagggttt aatagttatt tgtcagggtt tgagtacttt
    23641 tatattgttt tagttttact aactttagaa aaattaacgt ttcattcttt tatttggcaa
    23701 aataaataat ttcttttgta atataaagac cttttgcagg gctcaaattg atacttttaa
    23761 aacttaaaca ataacaatat gaaaaagaac atttttacac ttgttacgct tatcatggta
    23821 acctgtgcat tttcatttgg gcaaagcaaa aaagcctcta ttgcttttgc tacagaaaaa
    23881 tatgattttg gtaaaataca ggaatctgcc ggacaggtat cttatgattt tgtttttacc
    23941 aatgtaggag gcgaaccgct tgtgatatct aacgtacagg cctcttgtgg ctgcacttcg
    24001 cctaactggc caaaggaacc tgttatgcct ggtgctaaaa acaaaattac ggttacatat
    24061 aacccggctg gtcgtcccgg gaagtttacc aaatcaatta ctgtaacatc taatgccgag
    24121 ccggcttcaa gtacggtttt cattatgggt gaagttattc ccaaagagcc taccattgaa
    24181 gataaatttc ctcaagattt agggaaagtg aggatgaaaa ctacccaatt tgcattcaat
    24241 gctatttctc cggtaaaagt tagtacttcg agcgctgagt tatacaactc atctgattcg
    24301 gttgtagccc ttggggttaa aaatgtccct aactggatgc aggtaaaaat tattcctgaa
    24361 aaagttcagc ccaagcaaat tgctgttgta gaggttacgt atgatgcaaa agccaaaaac
    24421 gattggggct ttgttgtaga taaatttgac cttaccatca acggggtgcc tgcaaaagct
    24481 cgtatgaccg ttactggaaa tattatggat gattttacga cccttacgcc tgagcaaaaa
    24541 gctaatgctc caaaaattgt tatagatagt gctgtttatg attttgggac aattaaatct
    24601 ggagataagg ccaaacatga ttttgtagtg aaaaacgctg gaaaaagtga tttgctgata
    24661 cgtaaaattg cgacttcatg tggttgtact gctactaacc tgaaatcgca ggttgtaaaa
    24721 ccaggcgaat ctactgttat agctgttgaa tttaattctg ccggaagaac cggcaaccag
    24781 gcaaaacaaa tcactgttat ttctaacgac ccagagaacc ctaaaatgat gcttaatatt
    24841 aaaggagagg tgattgctca ataaaattag tgaaaaggga agggatttta taaaatcaag
    24901 aaaaacgccg gattttaaag ctggcgtttt ttttgtttat aattctttgc accacacctg
    24961 catcccgtga aataagcctt tcaaatctac ttcgtgcctg aataaaccaa acatggccga
    25021 accactgccc gacattgagg cataatcagc cccggcttca tatagctttt gctttatctc
    25081 agaaagtgtt ggatgttgaa taaataaatg tttttcaaaa tcattgacaa tgatgttttt
    25141 ccacatctca acaggttgcc tgataagatc aggcaaagct ggcctgtcag gcttaggggc
    25201 aatgcccttg tatgcctcgg gggttgatat gtaaatgtca ggttttacaa taacgatata
    25261 tcgtcctttt aacgaaaagt caatgggttc aatattttcg ccacgtccgg ttacatacga
    25321 aggcttgttt tcgataaaaa aggcacagtc gctgccaagc tgcagggcta tctcctgaag
    25381 ctttttgttg ttgaggttta agtcaaatgt agtattgagc atttttatca cccaggcagc
    25441 atcagcagaa ccacctccaa gacctgcccc aaaagggatg gctttatgaa ggtgcatgtt
    25501 gacgttggtt atattgtaat ggttatgcat gattttaaat gctttgatgc aaaggttatt
    25561 ttcgggtttt ccatcaatgg taatacccgt tgttgtaaat gaaaaatgcc cgtctgtttt
    25621 tacaatttct attgcatctg taaggttaac cggataaaat atcgattcga ggttgtggta
    25681 accgtctggg cgtttgcttg ttacatgcaa ccctatattg atttttgcat tagggaaacc
    25741 gaccattttt tttattgcga atatcgtgaa aaatattcaa atgcaagtgt ttgagcattg
    25801 cacgatgagt atttgtagta aaaaaatagt atagtggtag tatgtttcat gtaatagatt
    25861 gtgagcggtt tagaggggta caggcataca tttgttcaag atttattagt tgacaaatga
    25921 ggtttcattt aagttattgt tagtgagaaa attaagtggg gaaaattttg tttccctgct
    25981 tatttttctt aactttatta gaaatgcgaa actctaaacc aaattttatg ataaaacatc
    26041 tgacattact aatttttatt ttgatagggc ttaaaacttc cggacaatat aaattggtct
    26101 gggaagataa ctttgatggc acaactttag attcctcaaa atggggatat gtagatgaga
    26161 ttggagtttg gaataccggt gccaataatg aattgcagcg ttatagaaag gaaaatgtaa
    26221 cagtaggaag tgacggggca gggaataata gccttatcat tactgctaaa aaagaaagct
    26281 atgatgggta tcagtttaca tcgggacgtg tagataccag ggctaagttt tctttcaaat
    26341 acggtaaatt ggtagcccgt attaaagtgc ctgacctcgc taatggcctt tggcctgcat
    26401 tttggacatt gggtgttaca cctgatactt ggccagcttg cggtgaaatt gatattttag
    26461 agatgggaca tgctcaaggt attgccagca atacccaaaa caaatacctt accggggcgc
    26521 ttcattggga aagcggaggc aatcaggctg actatgccaa agcctatact gcaggcaaga
    26581 acctcaatga agattatcat ttatatactt tagaatggac acctatgtat ataaagatgt
    26641 atttagattc tatcgctaca ccttattata ctatgaatat accatctgct gacggcgaag
    26701 aatttactga ttatctgcat tatgttattt tcaaccttgc cgtaggggga caattgccgg
    26761 gtataacaaa tgctacaggt attacagcat ctcttccagc ctcaatgtat gttgactggg
    26821 taaagtttta tcaaaaagaa ggtgaaggag ctgtaaatat caatgatgaa gctatttgga
    26881 acaattttgg ggtatttgac gagacagggt cttattttta taaagcagat ataggttttg
    26941 atgctaaaat tgtagtaaac agtacttttg gtaagccttc aaattatgca gtttttgaag
    27001 gagatacagc attagcttat ttgcttggca gtaatgattc tttatcagtt tctgttaatt
    27061 ctttgataaa gaggaattta attttgttca atgagggaag tatttgtttt tatttaaaaa
    27121 caaacaattc acaaccatta actattgcag taaatgattt tgacggggga agttcttttt
    27181 atacattatc tggctttgaa gccgacaata cttaccataa agtggcagtg cctgtgaaag
    27241 gccttaaagg aaatgtaaac cttagtaaaa taaaaagctt gttcgccctt tctgctaaat
    27301 atccaggtgc tgctgagata tccatagaca aggttttttg gtcaacctca gttccatctg
    27361 caggcaatta tctgggttta tataccgaaa atcccaatat ttcagaaaaa ctggaagtaa
    27421 acaactcaag catttttctt tatatatggg acaatacatt gaaagtactt actactaatg
    27481 cctatgaagg aaagaatgtg atgtcttttg cttctgttgc tggaaaaacc tggtatggaa
    27541 caggtttgta cgccaatccg gcagtagatt taagtgcata caagaacgga tctttgcatt
    27601 ttgctttaaa aactactgat acaaaagaat tttatatagg tattgaaggg gcgaacagaa
    27661 ccaaaaaaac tataattttc aatgaaccta atgacccata cggatttgtt cgtgacgggc
    27721 agtggcatca gattgcaatt cctatgagcg aattttcaag cgttgacctt accgcttgcg
    27781 gcaacttttt tatcatgggc ggtgcacctt ctattacaga tattgctgtt gatgatgttt
    27841 atctttcagt agatgcctca agtattgaga atcctgcaat taatacatca gtcaaaaatg
    27901 ctcaaagtca aatgaatgat ataacggtaa gccccaatcc ttttcatgat gtggtaaaca
    27961 taaaaactac acgtccgata gacataatag acctttacac ttatgatgga aagattgcta
    28021 aatctttaaa aagcaatggc aacgatgtaa acattaacct ttcagagttg cagcctggct
    28081 tatatttttt ggtggtcaat aagcaacgcc aatcaacaag aaaactaact aaatattgaa
    28141 aaataactat ttataactaa ttaactatta actaaattca aaacaagctt atggtaaaaa
    28201 aaatgcttct tttggttaca tttctggcag gccttgtcca gctgtatgca caggacagga
    28261 ctataaccgg aaagattgtt gacgaggcag ggaatattct tcccggtgca accgtaacat
    28321 taaaaggtac tactaaagtc actgttactg atcttaacgg tgtatattct ctttcagctc
    28381 ctcaagaggg gggcactctt gtagtttcgt atgttggcta tacttctcag gaagtgcaaa
    28441 ttggcgagtc aaatactgtg gatatcaact tacaacccga tattcaggca ttaaatgaag
    28501 tagtggtggt tggatacggt gttcaaaaga aaagtctggt aacgggttct attgcaaaag
    28561 ttaaagcaga agacctggca aatcagccag cattgcgtgt tgaacaagct ttgcaaggta
    28621 aaacatccgg tgttattttc aaccagacgt ccggtaaccc cggagcacag gtcactgttc
    28681 gtatccgtgg tgtatcatcc aatggtaaat ctgaaccatt gttcattatc gacggaatga
    28741 aaacctcgaa atttgtcatg aacgaaattg accctgctga cattgaatct gttgaagtac
    28801 ttaaagatgc tgcttctgct gctatttatg gttcagaagg agccaacggg gttattatca
    28861 ttactaccaa atcaggtgcg ggcaataagg aaaaagttac ggttacctat gatggttatt
    28921 atggctggca ggttgcagac cctgtagcag taatgagcac tgcccaatac aaagattatt
    28981 ttagggaagc ttatgcttat gacaaaagta ttgaagcaag caattcgata aaaagtttcg
    29041 cactacctgc ttttgttgag aaggttagtt actggacaga tgcagcagac agcttgatat
    29101 ttaaatacaa tactccggct tatcagtatg aaagggtaag gtctatagct gagccagctt
    29161 tacagaaagc tttgaccaac cagcaaacct tattgtctaa tggttcaatc actcaaaaac
    29221 aatatgataa taatgtcaaa gccattaata ataaacttga agctcttgct aatttgaggc
    29281 ctttgacttt tgaacaatat aaggcaaatt tgttcaataa gaaattaaat tctccaaccg
    29341 ataaggctta tgccaatatt agggacaatg tggacaccac ttctaatggc accaattgga
    29401 tggatgaaat tttccaggta gccccgatgc aaaaacacca tatttcggta caagcaagct
    29461 ctgagaaagc aaatctgtac ttgtctgctt cttattttac gcaggatggc gttgtgggag
    29521 gagcaaaaaa taactttacc aggtataact tccgcttaaa tggtgacgtt caggctacca
    29581 aatggatgaa actcgggtca aatgtaacag tagcacagtc aaaatccaga aacctcccgt
    29641 taaatgacct ttatggaggt attatcagtg ctgcaatgtc tcatgaccct actattgtcc
    29701 catattggaa cgatacttct gaagtttttg cctattatcg ttctgttgga agaaattaca
    29761 ctacagaggc agcccgcgaa gatttgttaa ataaccgttt gttgaaaact gaagacggaa
    29821 ggtactacag taatactacc atgcctgtca atgaaagggt aaaccctttg gctattatgc
    29881 agttgtctca aaatgatgta gctactaccg agagggttat tggtaatgca tattcagaaa
    29941 tcaagccatt taaattttta acctataggc ttagttttag tggtgaactt acttatgtaa
    30001 ccaatgacaa ctgggggccc gcatacaaac ttaacgatac cagacaaaga gattacagct
    30061 atgtgtataa acaaatagcc aggtattata attcacaaat tgataacacc ctttcttttg
    30121 ttaaatcatt tggcgatcac aatgtgtcgg caactgttgg acagtctgct gagcaataca
    30181 taggatatag cgtaaatggt acccgtatca accttcagga gttgaatgaa agctggaact
    30241 atctggatgc tgtgcaaaga ccagatactc tttcaaaact cagagatgac aacggaggtt
    30301 tcaaagataa aaacacaaag atggcttatt ttggcagggt gaattacgat tttcgcgaaa
    30361 aatatatgtt tggtgctact attcgccgcg atggttcttc tttgtttagt gataaaaaac
    30421 gttggggagt attcccttca ttttctttag gttggaatgt tcacaaagaa agctttttca
    30481 atgtgccatt tgtttcttta atgaaggtta gagggagctg gggccgaaat ggcagcactc
    30541 aaaatgttga tgcttatatg tggatatcta ccaaaacagc ccaatcgtta ggaggtgcta
    30601 aaacgattgt aacaggtgtt accaaaaatg aagatttgaa atgggaaact tctgaacaaa
    30661 ccgatatcgg agtagatttt gggttcctta aaaataagtt gaccttaaca attgactggt
    30721 acaataaaaa gaccattgat ttattggcaa aaccaattct tcctgcctgg atggcttctt
    30781 cttccggaga tgttgcttat tataacgtag gcgaagtttc taacaaaggg ttagaatttg
    30841 atttgggcta ttctgacaat attgctgatt ttaaatacga tgcaaaactt actgctacct
    30901 atttaaaaaa cgaagttacc gattttaatg cacaaaggct tacaggttat ggggcacacg
    30961 gggctccttt tgaggctact gcttttgaag aaggtcagcc agtatggtat tttagaggtt
    31021 ataaagccga gggtattttc caaagctggg aagaaataag gaattatact tataccgatc
    31081 ctaatactgg tgtagttaca ttgattcagc ctgatgcagt gcctggagat gttaaaatta
    31141 aagatgtagg aggtaccgtt gaccctaaaa ccggaaaggc aagtattcct gatggaaaaa
    31201 ttgacggtaa tgattatact tacgtcggaa aacctatgcc ttcatggaca tttggtttga
    31261 acctcaatgg cgaatataaa ggatttgacc tgagcatgtt ctggtatgcc gaagtggaca
    31321 aagaaatata taacaacacc cttcgtggcg acttccttca cctgaacagg gctgaaacat
    31381 tctacaccaa cagatggact gaaggcagcg gtacaaatga ctggatgcgt gccagatata
    31441 ctgataacac tacaggttca acccaaattg gttttaacag cttgttcttt gaagacggta
    31501 gcttcctgag attgaaaaat atccaacttg gatatacttt gcctgtttcg ttgacccaaa
    31561 aagttcagat atcaaaattg cgtttttatg ttagtgcttc taatttgttg acttttacca
    31621 actataccgg ttcagatcct gaaattggtt atactggtga taacaattct aagaaccaca
    31681 acagttttgg tgttgacagg ggtatgtacc catctgcaaa acagtatatt attggtgtta
    31741 atattacttt ctaaaattta atgaatatga aaatgaaaac gatatataat acttttctga
    31801 ttttgttttc gtgcatggct atatttagct catgttctga agataggtta gatgttgaac
    31861 cttttggaaa atatgcccag gaccctgatc caaacaggcc tttggatttg aacatgattg
    31921 atggtgaagc caggggcttg tattcacaag tatcatggtg ctacaacacc caatggggtt
    31981 attctactta tgttactttt aacagtgctt cagatgatgc tgtttctggt ggcgctgctg
    32041 ccggtgaccg tcctgaatat gaagctgctg aaaagtttac ccttgaccct aataatttag
    32101 gcgtaatcaa tctctggaaa agattttatt ccggtattgg tagttgcaat aagcttttag
    32161 acaattatcc cgacaaagat attgacacgt taaaaagggc taaagcagaa gctaaatttc
    32221 tcagggctta ttattatttc aatttagtca atatttttgg gccggtccca atgccattaa
    32281 agtcaaccga tttggcagaa atgcctaata cccctatagc tgagatatat gctcaaattg
    32341 aaaaagacct caatgatgcc atagcttcca atggccttcc aaaaagtaaa aaacaattat
    32401 caagtgttga tttggctatt actcgtgcaa cagaatatac tgtccgcgca ttcctgggca
    32461 aagtttatgt ttaccattca accatgttga acgaaaacaa atggcaggat gctaaagcac
    32521 aacttgaagc tgtgaaaaac tccggcgaat attctttagt tactaatttc agaagccttt
    32581 ggtcaagcaa aaacgagatg aaagacggaa accctgaaag catcttcgaa gcttcatata
    32641 ccgatatata tggcgaaaac tggggaggtg tagctaccgg aaaccttgac atgcagttaa
    32701 tgggtatcag agatttgcaa ggttatcccg gtttagacgg tggatggggc ttctgtaagc
    32761 ctactcagaa acttgttgat gcttttattg ccgagagtga ttctatcagg ttagatgcca
    32821 ctgttgtggc cgcttcatgg ccagcttcaa tagcgcaatg ggaagctgat aaaaaattat
    32881 taccatcatt aaattcaaag ggtgctaaat atagcgagcc ttatgaccca actggctact
    32941 ggaatggcaa ataccgccag gacgaacatc ctctgaacgc cgatatgtat gctcaaaatg
    33001 agcttatata caggttttct gatgtattac ttcttttagc cgaaactgaa tatcatttgg
    33061 gcaacgaggc tgcagcttta ggatatgtta accagataag ggacagggta catttggccc
    33121 ccaaaagttc aacaggtatg cagctcttaa atgatattag aaaggaaaga caattagaac
    33181 ttgcaatgga aacccaaaga tattgggact taatccgttg gggattggca gctcaggaaa
    33241 ttcctaattt taaaacttca acaaaaggtt tgtggccaat accccaagaa cagttaaatg
    33301 ttgaccctct tttgaaacaa aacgaaggtt attaattatt ttccactcat aaaattaaag
    33361 aggctgccaa cactggcagc ctcttttggt ataaaccttc cacgttttga aaaacctgga
    33421 aagtcttttt attgaatgac accgtttact tactacacag ctcggtcaac acaccaaaag
    33481 ttgattttgg gtggagaaaa ccaatattca acccttctgc acctttgcgg ccttgtttgt
    33541 caatgagggt aacgcctttt tgttcaacag cagtcaatgc ttcatttaca ttatctacgg
    33601 caaaagcaat gtggtgtatc cctgctcctt ttttctcgat aaatttacca ataggtcctt
    33661 caggatcagt tgattccaaa agctcaattt tggtttgccc taccatgaag aaagctgttt
    33721 ttaccttctg gtcttttact tcttcaacgg cgtaacattc taaacccagt acatcctggt
    33781 aatacttaat agcttcatcg aggttgctta ctgctatccc gatatgctca atgtgcgatg
    33841 gcttcataaa taaactaaaa attaaaaatt aaaaatcttt gttgctcgcg gtaaaagtac
    33901 aaaataagaa ttttattaaa aatttatttt ggattgctgt ggataattct accaatagct
    33961 tgttttttac ccaaattaac cattaggtgg caatattttg ccaaactata tgattaattt
    34021 gggtcgttat aaacgggacc agctgattta gaaaatattt gatgtaattt gttaaattgc
    34081 tgaattacaa ttttttgatc aattaagtat tttctaatac ttaatttggg cttaatgaga
    34141 gtttgaaaaa agtaaagata atatcattaa aaaatggtgc aaaatagctt attttgaaag
    34201 ttttattaaa aaacgttgag gaagattgaa caaatgaaga taaaactcgc aaaatcagga
    34261 ctgttagtgc tttgcatttt ggctataata gtagccggtt gtgccaaggt aggaaatatt
    34321 cagggtggcc ccaaagactc tattcctccc gtagtagtga aaagctcccc gaaaaacttt
    34381 tcgaccaatt ttaaaggcaa aaagataaaa attgaattta acgaattcat tgagcttaaa
    34441 gacctcaaca aagaatttaa catgtcgccc cctgtgacca aaaaaccaaa gatatggctc
    34501 aatggcaaag taatggttgt cgaaattcgg gatacattaa aggatagtac cacctacacg
    34561 ttgagctttg gcaacagcat tgtcgatttg aacgaaggta acaagcttag caactttgaa
    34621 tttgttattt ctaccggtac atacattgat tcgatgggtg tacacggcaa aataaaagac
    34681 gccctgactc aaaagccctt aaaagagcct ctgctggcaa tgctttatgc caaccttgac
    34741 gattcggcct tttttctgca aaagcccatg tacaccggta gcgtagataa ggatgggaac
    34801 tttgcagtga acaatgtcaa gcctggaaag tacaaccttt ttgtattgtc tgacctcaat
    34861 tcaaacctga aatatgatat tggcgaaatg gttgctttct gcgattcggc attggtgctt
    34921 agccccgaat ttattactgg taagcataag attgaaatag aagaagatac tgcccgaaat
    34981 gacagtgtgg ttatggttgt tgacagcgca aagctccgtc aagccagaaa cagcttacac
    35041 gtcaacctta tttcctttat tgaagaagga aggaagcaat acatcaaatc atatacccgt
    35101 cctaacaggc ggtgcgttaa tattgtattt aacaagccct tgataaaaga cagtttgagc
    35161 atcgacctta tcaataataa aggagaaaat tggtatttga aagatcgtac caacttcaaa
    35221 gacagtcttt tcttatggat taaagatact acacttatta aaacagacac actgcgcctt
    35281 ggcattggtt attatgcctt tgacaaaaaa gattcgctta tctataaaaa agatacactc
    35341 ctcttcaggt ttatagacac ttcaaacccc aaaacgaaga aacccgaaga aaaagccaat
    35401 gtacttgtaa atgcaccgca gatcttcgag ttgaacggaa taataacgat agaatgccct
    35461 tatccggtct taaaaattga taaagaaaag ctgatactgg aggaaaaagt tgatactgtc
    35521 tttaacccag tggcgtatga tttaatacaa gacagcattt ttcagcgttt gtttgtggtc
    35581 aacaataaat ggaaagaaca gacttcatat aaaatgacaa tacttccagg ctttgctaac
    35641 aacatttatg ggcttccaca agatactata ataaatagct ttaccacaaa aagcctggat
    35701 agttacggga cattagccat taaccttaca gggcctaaaa tgccagtaat attgcaactt
    35761 ttagacggtg ataaggtcaa atatgaaata aaagctgaaa cagatggtat tattgacttc
    35821 aaataccttg agcctaaaga atacgttttg aggcttattg aagacaaaga caacaatggc
    35881 aaatacacct ctggtaacgt tttgaaaaag attcagcccg aaaatgttat atattacggg
    35941 caaatgataa agatacgtgc caactgggac atggaaatta attgggaact taaataaaac
    36001 tatggaacac caccatcctg caagaaaaaa acattcgcac caaagacatt cacatgctgg
    36061 cgataacctg ctgatagcta ctgtgctaaa tattaccatt actatagttg aggtagccgg
    36121 tggcctgttg tccaatagca ttgcgctatt gtcagatgca ttgcacaact ttggcgacag
    36181 tattgctgtt tttctggctt acgtttcaaa caaaattagc caaaaagggc cttcagcaaa
    36241 aaagacattt ggctacaaac gggtagaaat aatggcagcc atgctcaata gccttatttt
    36301 ggttgttatt tgtgcatatc tgttgtacga gtcgtggcac cgcatgataa accctaagcc
    36361 tgtccaaggg cttataatga ctattgtagc aacagtaggg cttttggcta acttatttgc
    36421 tgtatttctt ttacataaag acaaaaaccg aaatataaat ataaaggctg catatttaca
    36481 cttgttatct gacactgttt catctattgg cgtagtgata ggcggtttgc ttatctattt
    36541 ttacaaaata tactggattg acccaattat aaccatcatt atcagtcttt atatctttaa
    36601 agaaacctgg gggatattga aacaggccta tctgatcctt atccaggctg cccctgaagg
    36661 tgtggaaata gatgatatac ttgaagtgtt ggagcgttac gaagaaattg aaaatgtaca
    36721 ccatgtacat atttggaaac ttaacgatac cgaaattcat tttgaatgcc atatagacat
    36781 gaaaaataac gtgaggctca gcgaaactga agagttgatg caccgtataa aaaagatatt
    36841 attcgagaag tttaacattg tgcacactac tatccagttt gaatacaatt gttgtgacga
    36901 caaatctata atctacaaat gaaaaattat ttaaccttag caataatatt ggttttggta
    36961 atctcatgcg gacaacgaaa gacagctgtc ccggcttcta taatccccga agaacgtatg
    37021 attacaatac ttacagatta ccatctggca caaagcctca atacaaatta tagagtgcgg
    37081 aacgaaatgc taaaaaacat gcctgtcagg ctttctgaca gtgtcttaaa agccaatggc
    37141 gtaacaaaag cccaactcga tagctctctg acctattatg ctactgattt ggagcgtttt
    37201 gaaaaaatat acgacgaagt actcaaccgt cttaacaaga tgcaagctaa actacaggac
    37261 acccctaaaa aagactcttc aaaagtaact aaaaaatagc catgcgtaaa gtagctgcta
    37321 attatgtatt ccccgtatcg tcagctccta tacgcaatgg tattgttact ttcaacgatg
    37381 aaggaattat tactgaaata aaagacaatg gcggtgccct tatggaggct cagggtattg
    37441 aattttacaa tggtattctt gtccccggtt ttgtcaattg ccattgccac ctggagttaa
    37501 gccatttgca caagaagata tcccacggga atggattgat gacttttatt aatgaattgg
    37561 tatctatcag aaatgttgat attcgagata ttaaacactg tatttccgtt gctgacagtc
    37621 agatgtacag tgaaggaata gttgctgttg gcgatatttc taatacttct gatagcatta
    37681 ctgctaaaat ggcaagtaaa ataaagtatc acacatttat tgaactgttt gacctttatt
    37741 ttaaagatgt tggagagtta atcgctaatg gagccctttt acaaaagcaa tacgaagagc
    37801 tgggtttatc ttcttctatt gtaccacatg ctccatacag tgtgtctcct gaattatttg
    37861 taaatatagc ttcttttcat aaaaaaggtt cattgtacag catccataac caggaaacta
    37921 cagatgaaga cctgatgttt cttgaaggaa aaggcgtgtt gtttgatatg ataaacaaaa
    37981 tgcccaatag caggtttttg ccaacaggaa aagcttcatt gccctcttat tttgactatc
    38041 tgccaaatga atcaaaggtg ttgcttgttc acaatacctt tagcagggcc gaacatatac
    38101 aactattgaa aaataaggac ataacgttgg ttttgtgccc caatgccaat agatatattg
    38161 aaaacaggct gccagacata cctatgtttg taaaagaagg aataaaactt gctcttggaa
    38221 ccgatagcct cgcttcaaac tataaactct ccatcttaga agaaatgaaa actatagttg
    38281 ccagtttccc tgatatttca tttgaagaag tacttcaatg gggtacgcta aatggtgcca
    38341 aagcattggg cctggaggct gattttggta gttttgaagt gggaaagaag cctggaatta
    38401 accttatctg taattttgat ttccagaaga tgaccattga caaagatagt tttataaaaa
    38461 aactggtata atgattattg aatctattca aatatccgac ctttctgaaa ttgctaccct
    38521 tcaacccatg gactggcctg atattgtgcc cgatattgaa tactacatcc attccccgtt
    38581 ttgtagagct gttaaagtgg ttaaaaatgg cgttattgct ggtatagggg ccaacatctt
    38641 ttttgaaggc acctgctggc tggcacatat tattgttaac gaaagctatc gcaacaaagg
    38701 aattggaact gccattgttg caaaccttat gaattatgca aaatacgaag ggtatgacac
    38761 tcaaatgctt attgctactg agttgggtga acctgtttat aaaaaatcag gttttaaaaa
    38821 ggttacagac tatttgtttt ttaaacgtac tgaaccttct catatagaaa caaaatcaga
    38881 acgaataatc ccgtattctg ctcaatacaa aaatgccctt cttgaattag acagatatgt
    38941 aactggtgaa aatagaataa atatacttga aaagcatcta acatcttcat atcttattgc
    39001 taatcaaaat gaaatatatg cattttatat accttcatta ggtgagggcc caattattgc
    39061 ccaagacaaa gaggctggca ttgaactgat gcagtataaa tatgccactg ttgataaagc
    39121 cgttttacct gccgaaaacg ctactggtat tgaatttttg cttgccaacg gctttgttca
    39181 ggtatcaaaa tgttcccgga tgctttatgg caaagaaata ccctgtcatc ccgaaatgat
    39241 atacagccgc atccgcggaa atgttggata atattttaca atttaaacca ttgataaaaa
    39301 gttatttata aataatgtca cttttttaac gtcaaaatag tgttattttt ttaacgattt
    39361 gaaagtagaa gtaaaacaaa tgtgtaattt tgtcagcatt ttgattttta ccgaaatgat
    39421 ttattaatat ttaaacaaaa caatatgagt tttacacatg aagttgaagg aatgatttgc
    39481 cttgcacaag gtgcaaatca tggacctgct ccaataccct ctgaaggtaa atgggtaaag
    39541 gctaaagaag ttaaagacat ttcaggtttg acacacggtg ttggctggtg tgcaccacaa
    39601 caaggggctt gtaagcttac cttgaatgtt aaagatggta ttatccagga agcattggtt
    39661 gaaaccattg gctgtaccgg tatgacccac tcagctgcta tggctgctga aattcttcct
    39721 aacaaaacta tccttgaggc attgaataca gacctcgttt gcgatgctat caatactgca
    39781 atgcgcgagt tgtttctgca aatcgtttac ggacgtaccc agacagcatt ctcagaaggt
    39841 ggccttccta ttggcgctgg ccttgaagat ttaggtaaag ggcttcgtgg tgttactggt
    39901 actttgtacg gtacatctgc taaaggccct cgttatcttg aaatggccga agggtatatc
    39961 accaaaatcg gtattgacaa agacaacgaa attatcggtt acgaatttgt caaccttggc
    40021 cgtatgatgg atatgatcaa aagcggaact gatgcaaacg aagctcttaa gaaggcttct
    40081 ggcaaatatg gccgtatgga agatgcagtt aaagttattg acccacgtaa agaataggag
    40141 gaacttaaga tgtcacaaaa cgcacaatta ttcgaaggct atagccgccg tatcaatcaa
    40201 atcaacaaag tccttaattc ttacggaatt tcttcacttg aagaagctaa aaagatttgt
    40261 gatgacaaag gggttgatgt ttacaaaatt gtaaaagaca ttcaacccat agcatttgaa
    40321 aatgctatgt gggcatatat caccggagcg gcaattgcta taaaaaaagg acaaaaaaat
    40381 gcagctgata ttgcagctac tttaggcgaa ggtttacagg ctttttgtat ccctggttct
    40441 gttgctgacg accgtaaagt aggtcttggc catgggaact tagctgctat gctccttcgc
    40501 gaagaaacca aatgtttcgc tttccttgct ggtcacgaat catttgctgc tgctgaaggt
    40561 gctattggta ttgctaaatc tgcaaataaa gtacgtaaag agcctttacg cgttatcctt
    40621 aacggtttag gtaaagatgc tgctaagatt attgcccgta tcaatggatt tacctacgtt
    40681 caaacccagt ttgactacgc tactggagaa gtgaaagaag taaaacgcat tgcatattca
    40741 tcaggcgacc gtgctaaggt taactgctat ggtgctgacg atgttcgcga aggtgttgct
    40801 atcttccaca aagaaggtgt tgacgtttct atcacaggta actcaactaa cccaacacgt
    40861 ttccaacacc cagttgccgg tacatacaaa aaagaatgta tcgaacaagg caagaaatac
    40921 ttctctgtag cttcaggtgg tggtacaggc cgtacacttc acccagacaa catggctgct
    40981 ggtcctgctt cttatggtat gacagacact atgggccgta tgcactctga tgcccagttt
    41041 gccggttcat cttcagttcc tgctcacgta gaaatgatgg ggcttatcgg tatgggtaac
    41101 aacccaatgg taggcgcatc tgtagctgtt gctgtagctc tttcacaagc aaagtaattc
    41161 aattacgaat tataaattta aaattacgac ttgagtaaat gctggcattc agtatttctc
    41221 aggtcgtttt ttttcaatta aaaattgtca attacgaatt acggaatttt ctagctactt
    41281 ttaattcgta attttaaatt tttaattgaa ttgttatgaa gtctgagaat gttgttcagc
    41341 aaaagagctt tgatttcgct attaggattg taaatgttta taaatatctt caaacagaaa
    41401 agaaagaatt tgtaatatct aagcaattat tacgttctgg aacatctatt ggggctaata
    41461 tcgaagaaag tataggcgga cagtcagaaa aggacttttt gtcaaaagta gcaatagcat
    41521 ataaagaagc tcgagaaaca atgtattggc tgcgtctatt gctggctgca ggctacttaa
    41581 ctaatgaaca atctgagagt ttgaaattag atgctgaaga gatttgcaga atactaggga
    41641 aaatccaaat taccttaaaa aagaaactac acaattaaag attatcaatt acgaattacg
    41701 ttgtttttct gccattttta attcgtaatt ttgaattttt aattgatatt tatgactaag
    41761 caagaaacca tagacaaagc tatttcattt tttgaagaag gatatgcttg ttcacaggca
    41821 gtggtactgg catttgcgcc ccaatttaac atagatgaaa cagcagctaa gcttatatct
    41881 tctacttttg gcggaggaat gggccgtttg cgcgaaaaat gtggtgctgt gaccggaggt
    41941 ttcatggttc ttggtctggc ttatggcaac accgacccta aagacatgga caccaagctt
    42001 gctgcttacc agaaagtccg tgatataaac caactggtta ttgataaata tggaactacc
    42061 atgtgcagcg acatcctaaa acaaaatgtc tctgaaaatg acgttaaaca gcgaaatcat
    42121 cacgccatta tctgttataa agttgtaggt gatgttgccg gaatggtgta tgatatgctg
    42181 aacaaaaaat aaggccaggt gtgccccggc cttatcccaa ttaaaaacca aaaaatcaat
    42241 cgctcagaca cacagcaaat tcattttcga gctgaaaaaa gtgaactttt ccttctctag
    42301 ccagccatcc cagcgccata taaacatctt ttatgtccat tttggacatc tttttcaaat
    42361 cagtaacttt aagctcccct ttttccatta gcatttgcca gattttaccg gcatcaaacc
    42421 caatttttgt tttttccatg gtaatcgaat taagttaaac aatattactt caatacaaat
    42481 cttcgccatt agcctcaggc tggaaaacga cactctcaat cttcacaaac agcttccctt
    42541 taggggcatc aaacgagatg gtatccccgg ctttatagcc aattaaggca catccgaggg
    42601 gagagagtat agagaccatg cccttcgaaa aatctgcctg ctgaggatat accaaacgta
    42661 aatccatggt tttgtctgaa tctgcgaagc agacttttac aatggaattc atcgtgacaa
    42721 aatccggcgt aatctttttt gagtctatct tttttgcttt gcctaattcg tacgatagaa
    42781 aatcaagata ctttacatct atgctttttc tttctctcat tgtctgtata aggcctgtca
    42841 gctttgcaaa atcgtttatt gtcactacaa tttctttctt tttcatttcc acttgtttta
    42901 tcatatcttg tcctccattt ttataacatt ttaccaaact ttaatgcttt aaaaaatgct
    42961 atttttacct tgtaataaaa ctaaaaccga cacatacata aaacgaatac gatttatatt
    43021 tgtgtcggat acgatacaaa tataaggaac tttttgaata taaaaaatta gatgaagata
    43081 gctgtaaggg aaaacttaaa gccatcaggg ataagcaagg ggttg
//
LOCUS       DAOSPF010000028        41073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1012_length_41073_cov_7.498757, whole genome shotgun sequence.
ACCESSION   DAOSPF010000028 DAOSPF010000000
VERSION     DAOSPF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_41073_cov_7.498757"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..364
                     /locus_tag="PK252_07365"
     CDS             68..364
                     /locus_tag="PK252_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT14564.1"
                     /translation="MRGNKMPIGVYINDNKPFTSKKIELQPADRFYITTDGYADQFGG
                     EKDSKFMMKRLKKLLLDNHKLPFNKQCEILEHTSNNWRGECYQVDDMCVLGFEF"
     gene            complement(431..907)
                     /locus_tag="PK252_07370"
     CDS             complement(431..907)
                     /locus_tag="PK252_07370"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT14565.1"
                     /translation="MSKVVIIMGSKADLDWANQIVKVLDSFDVASVCRIASAHKVPLK
                     CYDLIKEYEKENVVFITIAGMSNALSGFADAQTCCPVIACPPYSDKFGGADVFSSIRM
                     PSGVAPMTILSPENAALAAAKIFGLSNPAIQKKVVDFQEKKRKELEEADRQLVSKG"
     gene            complement(900..2822)
                     /locus_tag="PK252_07375"
     CDS             complement(900..2822)
                     /locus_tag="PK252_07375"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOT14566.1"
                     /translation="MEDLFKKLEKKRISLPDDALIMLSGCKSYKTFDTVKELASAAVG
                     GDQASYEVKYQIEGKGEMTEAIVHRVTNGISVNYTEAYMRRRDPDTMVIADDYPSDKE
                     RFADRFGYSFDVLRKETIEWLQKQDLAVFFYFAGREKIGSGGIAIAPANAGFFAMGLA
                     MLQSIIPVNDLPEEFAIESAIYIAPTFRHTHFAGKQIVVHKRSEGLHEIFSYNLYPGP
                     SAKKGLYGALLTKGEREGWVTAHCSTVQVVSPYDNITTFMHEGASGGGKSEMLQNIVR
                     EENGQVLIGENTVTGEKRLINFPLFCSFNPVTDDMALCHPSIQKNNGKLTVLDAENAW
                     FIRVDSINDYGDDPFLEKLTIKPKEPLLFLNIKSNPECTALIWDHTEDSPGKKCPNPR
                     VIVPRNIVPNVVGKPVTVDVRSFGVRTPPCTKENPTYGIIGLFHILPPALAWLWRLVA
                     PRGHANPSIVDASGMGSEGVGSYWPFATGKMITHANLLLEQIINTPRMRYTLVPNQHI
                     GAWKVGFKPQLLMREYLTRRGNAKLRSDQYQYARCPLLGFELNYLTIEGAKIPSRFLQ
                     VHKQVEVETSGYDAGAEILYDFFRKELQQYLTPELSNTGRKIIEACLAGASVEEYLSI
                     IPMDYQYSFFKNSENE"
     gene            complement(2927..3478)
                     /locus_tag="PK252_07380"
     CDS             complement(2927..3478)
                     /locus_tag="PK252_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT14567.1"
                     /translation="MNLIGERIKKKRELLKLHLNELAEKVDISPSALSQIEKSKSFPS
                     IMTLKAIADALHTTIGDLVGENDSMANHPVVSKSEIKFVDQNNTGTIVFLLSNHDPNK
                     LMDTYLVRFAKASGIDGLFKTKHGQVFCHVLSGEIRFDLADKSYNLKQGDNIYFNAKI
                     SFNAINSNDGLSELLWVQSPPNF"
     gene            3928..4380
                     /locus_tag="PK252_07385"
     CDS             3928..4380
                     /locus_tag="PK252_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT14568.1"
                     /translation="MKIVDNLNLDQATKEKLFQSEEACFAMLAGMKWTEGYICKKCGH
                     DNFCDGKTPHSRRCTRCKNEESATAHTLFHNIKFPITKAFYILFEVANNHKYSIFDIA
                     TQINVRQLVCWNFKNKIEEKYNRLVRFNNIEAVTFNDLIIKEANSPFA"
     gene            complement(4392..7064)
                     /locus_tag="PK252_07390"
     CDS             complement(4392..7064)
                     /locus_tag="PK252_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOT14569.1"
                     /translation="MSDDLDNIPDPEELENLPDETSAEAAPGSHFQDKVIHTTVLGGM
                     YKDWFLDYASYVILERAVPHINDGLKPVQRRIFHSMRRLDDGRFNKVANIIGHTMQFH
                     PHGDASIGEALVQLGQKELAVECQGNWGNIFTGDSAAAPRYIEARLSKFAMEVIFNPK
                     TTEWKLSYDGRNKEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELLDASIAVLK
                     NEPFTLYPDFPTGGLADISKYNDGLRGGVVKVRAKINKLDKKTLVINDIPFGKTTSTL
                     IDSILKANDKGKIKIRKIDDNTAEKVEIIVHLQPGVSPDVTIDALYAFTDCEISISPN
                     SCVIDNDKPRFIGVSEILRANTYRTLELLRQELNIRMAELLEDWHLSSLEKIFFEEKI
                     YRELEKETESWELTIDAIDKAFNPFRPRFKREITREDLLKLTEKPVRRISRFDIKKAD
                     ELIKGLEKEMEQVQYDLDNIVTYTINWFKNLKKKYGADKQRLTELRSFESIEATAVIA
                     NNEKLYINRADGFIGTNLKKDEFVCDCSDIDDILVIRRDGTYLTTKVAEKTFVGKDIL
                     HVSVFKKNDTRTIFNIIYFDGESGFSYVKRCAINGLTRDKEYNLTQGTPYSKILYLTV
                     NPNGEAETVKVKLKPRPRLKVLDLIFNFAELAVKGRGAIGNILTKHAIHKIELKEKGI
                     STIGGTAIWFDNDTFRLNTDERGTYLGQFIGNDKILVLNQKGEFYTTSYELTNHFDDD
                     IVFIEKYTPDKVFSIVYFDAEQQFYYVKRCTFEMSERPQNFIDDNPKSKLILLSTEFF
                     PQFKIDFGGKHKNRPAEIIDVEQFIGVKSFRAKGRRLTTFEVKTVAEIEPLQKEIPVK
                     PDESQELQSGIADIKEEDIFKNTSSGKPIEPSLF"
     gene            complement(7126..8973)
                     /locus_tag="PK252_07395"
     CDS             complement(7126..8973)
                     /locus_tag="PK252_07395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOT14570.1"
                     /translation="MEQVNYSEDSIKTLEWNEHIRRRPGMYIGKLGDGSSPDDGIYIL
                     IKEVIDNSIDEYMMGYGKEIVIDVIDNEVVVRDFGRGIPLGKLVEVVSRMNTGAKYDS
                     KTFQKSVGLNGVGVKAVNALSESFTIESFRENISKKVVFNAGNKVEEFPETTTSEKNG
                     TRVTFKPDPALFGNYHFIQEYIETMLRNYTYLNAGLVIKFNGQVFTSKNGLLDLLTDN
                     MSEEGLYPVIHLKAEDIEVAITHNNEYGEDYFSFVNGQHTTQGGTHLQAFKEAYVKTI
                     REFYKKEYEPSDIRGSIVAAIAIRIQEPVFESQTKTKLGSKDMVEGGVSVNKYITDFL
                     KRDLDNFLHKNASIAEALQKKIVENEKERKAISGVQKLAKERAKKANLHNKKLRDCRI
                     HLTDKDERRLETTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNTYGLTKKIVYEN
                     EEFNLLQAALNIEEDYENLRYNNIVVATDADVDGMHIRLLLITFFLQFFPDLIRKGHL
                     YILQTPLFRVRNKQKTFYCYDEKEKEKAVKSLGANPEITRFKGLGEISPDEFKHFIGK
                     DIRLDPVRLRKDDVIHGLLEFYMGKNTQERQDFIIDNLRFEEDLLELAN"
     gene            complement(9145..9918)
                     /locus_tag="PK252_07400"
     CDS             complement(9145..9918)
                     /locus_tag="PK252_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14571.1"
                     /translation="MFFNKKYRILKKMHYVADQQGIIKRFLKESENWGSHLEHCKAAI
                     KKDLSGRGRGKCAVLGSGWLLDIPIDALLEHFEKVYLFDIIHPTQVKHKYAHNPKVVF
                     VELDITGSVITQMFEAVQYYKSTKYVKNIDTYQFEGFQYPEEFDFVISANIISQLATL
                     LVEYITPYGLYSDEELALLSRKVEESHLQSLPVGKTFLITDYEELVYSKDNILEKQRP
                     LLQVSFNSLPVAESWQWKFDRDDYYKDKNVVYNVIALQK"
     gene            complement(9970..10710)
                     /locus_tag="PK252_07405"
     CDS             complement(9970..10710)
                     /locus_tag="PK252_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT14572.1"
                     /translation="MRKLLTTAWIWFFGVVSLVVLFIPFFVLWIISWPFDQRHRCNMY
                     YTSLWAMLYLKILPIKRVEVIGKHKLKPCMKAVAISNHQSMADILVLFAVFPYYIWVS
                     KKENFQAPILGWVMYLNGYISLDRNDPKTFPKMYKKISEKLAKNFTIMMFPEGTRSKN
                     CEIGRFKDGAFRAAIENKASIVPIVLDGTGNAIPKEGIAVNKGDRIIVKVLDEVPYDK
                     FPTYNPAELKEYFRDMIAKELAALRSEN"
     gene            complement(10981..12378)
                     /locus_tag="PK252_07410"
     CDS             complement(10981..12378)
                     /locus_tag="PK252_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT14573.1"
                     /translation="MPNFFKNYFSGYHKLGGIKELLAIALPMIVSTACDGAMTFTDRL
                     FLAKVGSEQMNAALGGGVTMQMLMFFFVGLTGYSTALVAQYFGAGEKHNSSKAAFQAI
                     LITVLAWPVILILKPLVVSFYHFSDISQVQLGYQVQYLNILAWGGLFGLLRYTLGCYF
                     SGIGKTQIVMKATLAALVINVILDYLLIFGKFGFPSLGIQGAAIATVCGALGALIILV
                     LTYFQKKNVVEFSVFKSFHFDWDIMKKLIKFGYPAGLELFLLFCAMTMLVTVFHSKGE
                     VIATASTIMFNWDLVSFIPLLGIEIAVTSLVGRYMGAGRPQVAHRAALSGVKSGFFYS
                     IVILFLFLLIPESLVRVFSPEGLSSGFEETVPIAVAMIRIAAIYVLAEAIMVAIIGAL
                     RGAGDTMFTMIASVTAHWVLVPILYLSLHVFNISVVLSWFLMVIIFMVFCGVLYLRFQ
                     GGRWKQIKVIEVEAK"
     gene            complement(12606..13364)
                     /locus_tag="PK252_07415"
     CDS             complement(12606..13364)
                     /locus_tag="PK252_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOT14574.1"
                     /translation="MNGVWVNALVATFAVSAIAGIGIVFFVLKHDVLKQIMFIMVSFA
                     VGALLGNVIFHLLPEIFEEHASENIMGLALAAGLLLFFITERVFHWHHQHNLEENPCG
                     HEHSFGYVTLIAESIHNFTDGVLIAASWLTSNEIGMATTIAVMLHEIPQEISNFSILI
                     HAGFKKRKALMFNLFSACTAIVGAVITLLVGQSARDIIPYILPFAAGGFIYLACSDLI
                     PELHKQPFRTFVLQLIAISAGIVLMMAMSTGHSH"
     gene            complement(13429..14736)
                     /locus_tag="PK252_07420"
     CDS             complement(13429..14736)
                     /locus_tag="PK252_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT14575.1"
                     /translation="MKILTLKNTKISNTVRKVAVFLKQNGRVAMLASRGFFAYKSTMR
                     AASLTYFTLLSMVPILALVFGIAKGFGMERMLQREIMNKLAAQQEVALWVINLAKSIF
                     SKARGEWIAGVGFIILFWAVIRMLNNIENAFNDIWHIKKGRKFTRKLSGYFALIFFAP
                     IFIILSSSATVYLTTHIKTIISSIAFLGFFAPFIKFFISLIPYILVWALFTMVFMTVP
                     NTKVRFGPAIVAGILSGTMFQLTQWAYVKFQIGVSGYNALYGSLAALPLFMVWVQTGW
                     LIVLFGAEFCYIAHNIEQEYTNIRLTELSTNTKRALYLMITHVIVDKYAKGEKAPSIA
                     EIAIKTKLPNFLIADLITELVDSGVLIQVYTDLDEEQAYQPAVDPMLITIGFVIEAVN
                     TKGVKNDIVAEALKSKPYEYIVLKMNKAMEKSESNILVKNILL"
     gene            complement(14758..17706)
                     /locus_tag="PK252_07425"
     CDS             complement(14758..17706)
                     /locus_tag="PK252_07425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT14576.1"
                     /translation="MKLEVVYILTLHRKLGYLVVPYIIERKAQQSFFQLVEYLTVDNI
                     EPYKNILDETDLQLVKITSGYSEKNIHKNFAKRNSLKEFFDTLDQNLVEEHIRPYIEK
                     RIISCVELMVAFGKKVYKKDQKYNVVHDDEQIKLTSSLVHPVFNFVRTDVDFKYFLSL
                     RCEDKDIKLFGKSYFVLSNQPAIVVVENVLYQVSQIDSKKLIPFFSKDFIKVPKTSEK
                     SYLETFVRNAILHHPVKVVGFRVETTDYKKKAMLVLDSGLSGQPVFMLQFFYGEVKFE
                     YGSDVIGHVGLTDADDKVCFYKTLRDLAWEESMKDKLVSFGFRYIDRGAFVLHALEGE
                     PEWQFYEMVCWINSNTRMLQDEGFELDQMKFDKNYYLHEVLLKLNVNEAHDWFDINAV
                     VTIGEYVFPFSRFRKHIVHNIREFTLPNNQIVILPLAWFSRYRELFVFGEDDDERIRL
                     KKHHFNILKESMGVETRQMIMGIQEKLSLSENKYLKEPQGLQAKLRQYQKTGVKWMFQ
                     LYKNKLGGCLADDMGLGKTLQTIALLLKVREEELRNPAIAQTITRTGPRQLSLFDNPD
                     FTGSRCIGASLIVMPVSLLHNWETEFKKFAPSLHVVKFFGSQRMKAYEELLRADIVLM
                     SYGVVRNDIDHLSKLMFKYVILDESQMIKNPDSKIYKAILRLQSEYRLVLTGTPIENS
                     LTDLWSQLNFLNRELLKSQNYFREEFVIPIEKYGNQAKQQKLKMLINPFILRRKKEEV
                     VKELPPLTEQTIFCEMDEEQAQLYEQEKSSVRNMIMNNIDQHGFEKSAIVILKALNRL
                     RLMSNHPCLVDASYQGGSGKYDEIIRSIENVIVENHKVLIFSSYVKHLNLIAGYLEHN
                     GYKYSMLTGATHDRKQVIDRFQGDEENNIFLISLKAGGVGLNLTSADYVFIIDPWWNP
                     AAENQAISRAHRIGQDKKVFVYRFITTKSVEEKILKLQEHKSGMAKAFINSNNPFKAM
                     QREDIESLFL"
     gene            complement(17844..18107)
                     /locus_tag="PK252_07430"
     CDS             complement(17844..18107)
                     /locus_tag="PK252_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOT14577.1"
                     /translation="MELFTEIVGYLAAVFGTALMLPQVYKSIKTKRVDDISMSMLVVY
                     VINCTLWEVYGLLIHAMPVILCNIIALGIGIVQLVLKFKYRTN"
     gene            complement(18285..21128)
                     /locus_tag="PK252_07435"
     CDS             complement(18285..21128)
                     /locus_tag="PK252_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT14578.1"
                     /translation="MKPFLYLVADDLYKRFGEKLNTVRLVFPNRRSILFFKKYLSEIV
                     QKPVWAPGYFTVSDFFGQYTNLSKANDLLLNFTLYKAYTDVRQTGESFDNFYYWGEVM
                     LSDFDDIDKYLVNTHLLFRNLAAYKNINETFEALTEEQLETIRRFWQHFEKDNVSKHQ
                     HDFSNLWLNLEEIYSLFKERLRQQGFAYEGMIYRDVAESVMAGKFQSNDFEYTAFIGF
                     NALTEAENIVYKHFAANGKTLFYWDYYAEMLQEGHESGFFISENLRNFNPAINFNFPQ
                     LSNDKKIECIGVPSEVGQAKLAGNLVKKYLGKDAVPEETAILLTDEQLLPALLSAIPS
                     DIPDINITMGYPAANSAFFSLIEVFARIHLKNKPGEGGQNFFNHKDVFEVLMHPLVMQ
                     TSAEVAVGLRKQIVEKNLTYISSVQLRKDELLSLIFSPLKEAKEFIKTLSDLSVFFAN
                     EFIKNIDLTQEEPAGIEQQSIASILPVVDLLQQQLTLIGEELISLEVAIRATMKALRS
                     VQIPFEGEPLRGLQVMGFLESRALDFENVVILGFNEGKLPKNNFPVSFIPYSLRRGFK
                     LPTMKYRDAMYAYYFYRLLHRAKNVTLLYNSAPDSRSASEISRYALQLQYLHKMDLQY
                     KSQSFAITPGRVEPIQVAKDTAVIAALDDIVQKKYFSPSALSTYIDCPLKFCYKYIKR
                     LKPEEEVTEDVEGALIGLVVHAVMQHLYKPYIGQLLSAEIIDLLIADKKRIQELIHAA
                     LLEELKDEERMMDGKVVIIKEVIQRYVIRVLEMDKEIAPVHIMALEQEVVQPFIVNIG
                     PEKKEIKIGGKIDRIDEVQHIIRIVDYKTGNVAKSFTEVKDIFAAGVTENSKKEIFQT
                     GLYALIASKQGGLPAVPVPCLYALRSKFDTSGQTYFSMNRQTITDFQPLLATFEENLL
                     GLLKDIWNVEKPFVQTTAKKTCEYCDYKQICHK"
     gene            complement(21408..22136)
                     /locus_tag="PK252_07440"
     CDS             complement(21408..22136)
                     /locus_tag="PK252_07440"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOT14579.1"
                     /translation="MQVFYSNEIDGKHCFLSEEESKHASKVLRLRKGDEVCVTDGNGV
                     LYKGSMAEINSGKCIIWIEEELKRQRRKAQELHLAVAPTKNADRIEWMLEKAVELGLS
                     AFTPIVCSRSERRNINIERLERIALSAMKQSLNLFKPVINEAVAFDKFITANKSGSLY
                     IAHCIEADAIGENYKTESLFTIKPALGENMTVLIGPEGDFTPDEVRKAVNAGYKPVSL
                     GESRLRTETAALVAANYFYIQVNS"
     gene            complement(22140..22634)
                     /locus_tag="PK252_07445"
     CDS             complement(22140..22634)
                     /locus_tag="PK252_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT14580.1"
                     /translation="MKRIGLISDTHGYFDGDIANFFADCDEIWHAGDIGNVETLDYIK
                     MYKPVRAVYGNIDGFEVRHECKEWLHFDCEGLGVFITHIGGYPGRYEYGIKELLLKLK
                     PGLFISGHSHILKVINDKQLGLLHINPGAAGISGFHHVQTAVRFTVEDGQPKDLEIWE
                     KKRE"
     gene            complement(22791..25145)
                     /locus_tag="PK252_07450"
     CDS             complement(22791..25145)
                     /locus_tag="PK252_07450"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOT14581.1"
                     /translation="MKLSLNKLRLGTRLFLSIAVSLVVTLTVFVIFLSRDTTKMTTDL
                     MDKNIQLLVDAVFEDMDISYKNHLEIVNTSMKLAMQEFKGKFTYNANNQNFVEIKNQE
                     NNETKMVRLGNLMFNGVGLVNNTAHVDYISGLMGCSSTVFAVIPEGMVRISTTLKDET
                     GKRAVFTYIPETSPVYQAISQGREYTGLAKVLNKWVLSSYRPVFDDNKKVIGAIYVGL
                     SDNIYNDMKAKLSKIIVGEGSYFFIIDTTATEIVHPKLEGENLYNTADENGYLFMQDI
                     CRLKNGKIKYDWKNPGEPVARTKTAYFKHYRPLQWILALGVYDDDFLKAAKEQRAYLI
                     GFGILLLAVLSVIVYVMSLVIRRNIERLENETKMLIDSALDGDLKKRSHAKNLSVEFV
                     PIVEGINKILDTVIAPLTIAAEYIDRISKGDMPELNTKEYNGDFKALMANLNDLIVSQ
                     NTIIDKAKEVASGNLTISLEMRSKNDELMEALNDMVKSNASMINEFKTAIENIVQAGE
                     QLMAVAMQISQGSTEQAASTEEVSSSMEQMVSNINQNADNAKQTENIAVRASTDILDG
                     SKAVTTTVDAMKQIADKISIIGEIAEKTDLLAINAAIEAARAGEQGKGFAVVAAEVRK
                     LAENSQNAAKQIDELSKSSVKIADESGTLLQKIVPDIQKTALLVQEISASSMEQNSGA
                     AQINNAIMQLNAVTQKNAAAAEEMSASAEELNSQAEQLRQLIAFYKTEVEDHAASRFV
                     KKEKIVKPVQQPHKTNKPEVKSPAIKADIKMGHEDDVDKHYDTF"
     gene            complement(25157..25639)
                     /locus_tag="PK252_07455"
     CDS             complement(25157..25639)
                     /locus_tag="PK252_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14582.1"
                     /translation="MNRTYLSFLVYGQYFAVDVVKVLEVLQKQNIVQVPNAPAYVKGI
                     INFRGEIVPVFDLRSKFNFPENDAKSSFVIIVLDLSGQNGSFRIGAIADKVKDVITID
                     DNAIKPVPQMSEKFNTEFLQGIFKIDDSFILLLNVDKVFSNAETPDIQNVQEKEVTNL
                     "
     gene            complement(25675..27684)
                     /locus_tag="PK252_07460"
     CDS             complement(25675..27684)
                     /locus_tag="PK252_07460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT14583.1"
                     /translation="MDPILSKFRDKFIEEAIGLLDRLEKDLLELEANPQDKELIESAF
                     RAMHTIKGVSGMYGFDFISEYTHHMESVYQAIRDAKLKFDKAVFDVTFASVDHIRKLL
                     SDERLEDKQNQANHNKLLSEISSILDNKNDAASGHADPEPIPAGDEGIESTWNIIVRT
                     TEMMYFRGINIMNIFEELATLGKFQVARIESLSDEENDYWNIFLISSASEDDIREVFM
                     FIEDDCIIQNLSKGNLLDKEKKTNNVMNEGEKSILRFVEDAKFPKKEVTEPDIIAEKP
                     VKKEEKQELQNFVRISVDSRKLDNLMYLVSELITVNSQIQVNLNDDRYSAMRAQFEKI
                     DSLSKLFRNNALDIRLIPLNDTVLRFQRLIRDLSKQLNKKIEFRTQGTETELDKNTID
                     SLTEPLMHLIRNCIDHGIELPEVRKSKGKPETGMIKLSAFQSGNFVYINIEDDGTGID
                     LEKVRQKAVDKGILKANDKLSDKEIYDLVFLPGFSTAQSLTEVSGRGVGMDVVKQRIR
                     DLRGEIIVNSVPGKGTTFTLKLQQSLAIIDTLLFKVEKSYFIVPISEIVICQQMALVE
                     LDSCRNTGTIPYNDTLIRFVDLRESFNLGGAYNKKVKLIVLNQNGKNIALLADKIIGE
                     HQAVLKPLGQTFENQNYVSSASQLGDGGIAFMLDIASLINVAQKV"
     gene            complement(27803..28471)
                     /locus_tag="PK252_07465"
     CDS             complement(27803..28471)
                     /locus_tag="PK252_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14584.1"
                     /translation="MDAKKIKVFVVDDHDLFREGVNLLINGSSKAEVVGEAVNGEDFL
                     EKLDSLKVDIVLMDIAMPVMDGIEASKKAHEKHPDLKIIALTMFGEEKYYFKMLQAGV
                     KGFVLKSSGISELLNAIVDVYNNKSYFSNELLMNLVEGFKAQLSKDSKVAVNNMQHLS
                     AREVEVLRHIASGLSNEEIAEKLNISTTTIRTHRSRVMAKTGCNNTASLIFYAIKNDF
                     VKVP"
     gene            complement(28474..29916)
                     /locus_tag="PK252_07470"
     CDS             complement(28474..29916)
                     /locus_tag="PK252_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14585.1"
                     /translation="MSKNSNNSILYVDDEVNSLDVFRLYFENTYNVYVAQNTKEAEEL
                     LRTVPVKVLISDYYMPGESGIDFIQRINPLYPDVVKIMFSAFVDREIILGALNKASVY
                     RYITKPWNANEIRLAIDSAIREYDLKHENQYLFEELRVKNSELYDTLQSLKQSEGKIR
                     KVIENADEGIFFINDEKQIIDANPAFFKMLGMEALNGQFSLLTREIENRIPVLLNLPK
                     EIASREVERYKEIEFNDGAKVKYFEISSSYADAENKDIIVSLVKDFTEKKLVDKKLLD
                     AIIHTQEEDQKRYARELHDGLGPILSTLKMHLEWMNNPEQPVNKDKIIQHSIKTVNEA
                     VNTVKEIANNLSPHVLQKFGLAYAISTYINKIGLNPNVEISFHTNLKEQVNETIETDL
                     YRIVLECLNNSLKHAKCSSVLIKLKKYEHSLFLGFMDNGKGFDIAKVRESNNGMGLYN
                     IINRIRHLNGKYKMISKPNQGFILTSLINC"
     gene            complement(30245..34384)
                     /locus_tag="PK252_07475"
     CDS             complement(30245..34384)
                     /locus_tag="PK252_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT14586.1"
                     /translation="MKKKLYSALAFFLFVNISLFASEKVLKIDIYSYKDGLTTSSVNA
                     VFKDSKGFLWLCSGNGLFRYDGYSFRNLSTITNADLNCEVYSVAEDNLQNIWIGTKGK
                     GIFVLLNKTFELVHLNLKLSEEVVVNQILFLKGKVWVASNIGLLEINKNINLSDGTPI
                     SYKRLIPDLANRTSQDKVINCLYHKAGSKILWVGTNAQLYAYNVEIDEFSEIISFPQN
                     SIRCLSVLDNKILAGSWDGGVFLVDTLTLKKTSTPFIDYVNKIVGEKRVKAALPDNNG
                     NLFVATYGSGLYAFNNANASFSYINYRNDKNSKENIRSNIINQAYIDNSGILWLSMNQ
                     PGLAKLYSQESFISYFSTKKSSDLVNEFLSVQQSIDKNKLWVATNGDGIYLFDTKEKS
                     FLQFNNTAGSRLRLQSNGASYCFQDSKGNLWIVSGRKGIYVVPGKKALQLFNGKGGGV
                     LPLDANLLFSSDSRANSYITKFYEDSKGYIWVGSWGTLYVLRMKEGFQNASTPDELAQ
                     NCTVKSIFSDEQKDEFDFPIFPAITMCEVQKNEIWVGTSGGGVCSVKELPGFKFSVEP
                     LTYINKDLPQLNVRSICKDSKQGIWIGTGAGLCYLKTPWAKLKVLSKSDGLSSDDIGN
                     IVEDKKGALWAATNFGIIKINPENFSVQNFLSAHNDDFNQYILNAGCYDALGNVWFST
                     PGALASLNTDSTYSSGANLPFYFTDIKIDNQTVVPMQKVDGHTIIRCDINECNEINVP
                     YNHTLSVEFATLNFKAAGQILYRYKIEKSKEWIMLGPNQRNISFSSMKPGLYFLEVQE
                     TGMAKKTNSKLLKVRFLPPFWQTKTAFVIYFFVILILFLIYRRLTIQKVLQQSVIEKE
                     RYERKKLEELDKMKTEFFSNISHEFRTPLSLIISPLEKLAKSNELSEKNKEKINIVLK
                     SSNRLLKLTNELMDYSKIEKNLLVPEFYLCDIVALTKEVCQLFNSLAESMNIDFKVNN
                     PQEAIEVPMDKMMIEKVIFNLLSNAFKYTPAKGTILVNIENVNNFNKDFVKVSVVNTG
                     EGIVKEHLSKVFDRFYQVNNVQNRNIAGTGIGLSLVKSYVELHNGMVDVKSEPNVETT
                     FEFYLPFEQAAFTVNKDKNTSFAINHEQGKNLEPKPTYHYHLLIVEDEEEIRNYIVDE
                     LKTDFSISVAADGEEGLRIANEMMPDLIITDVMMPKISGIELCKTLKNQLGTSHIPII
                     ILSAKANINQQIEGLEMGADVYMVKPFSVDHLKIQVLRLIGFKESIYSRYLKETALIP
                     EGAVTSKLDDEFLKKLTTFIEDNLTDTNLNVDQLADCVALSKVQTYRKVKAITGLSIV
                     EFVRTVRLKKAVKLVLEKRLSYTEIAYETGFSSPSYFTRCFHDHFGKTPSEFAADYGK
                     G"
     gene            complement(34518..36245)
                     /locus_tag="PK252_07480"
     CDS             complement(34518..36245)
                     /locus_tag="PK252_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT14587.1"
                     /translation="MKFVKVFLLLIVFLVLAVLCYYKLELYEYDQTTICNPINISYRF
                     TLSGPSFREAASPTVVLFKNEYFLFLTRSGGYWYSKDLAKWELITTEDLPIEDRSPTA
                     VVLNDTMYFMASFGSPSIYRSADPKSGKWEEIRDNFPYGQMDPCLFVDDNKRMYLYYG
                     CSDKSPIYGIEINTKNFNPIGKPKELIDSHKDDYGWERKGDNNELKENPWMEGAWMTK
                     HNGRYYLQYAAPGTQYKGYCDGVYVSDSPLGNFKLAPNNPISYKPGGFIAGAGHSSTF
                     QDRYGNYWHISTMTISRKHIFERRIGLFPMFFDKDNELYTYTAYGDFPMILPRKKVSS
                     PEDLSPKWMLLSYGKPVMVSSELPDYPKENAVDEDVRTYWSASSGERGEWIIVDLQKV
                     CIVNAVQLNYAENNTTITGRNTDIYCQYDLEASLDKKDWETIKSRKGSKKDLPHDYIE
                     LKKPIKARYIKLTNRHVPDGTFALSGLRVFGNAAGALPKAVDKFSITRSDTDRCIVKL
                     KWQINPEDVGYTIRYGSKQDKLYHFYQVLGSDSLIIRSLNRFEKYYFTIDAFNENGIT
                     KGTKIVSVD"
     gene            complement(36304..38772)
                     /locus_tag="PK252_07485"
     CDS             complement(36304..38772)
                     /locus_tag="PK252_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT14588.1"
                     /translation="MIKKILCHALFFSFILVAQNALAQQGGKLKLWYNKPAKVWNEAL
                     PLGNGRLGAMVFGGPEKELVQLNEATFWSGGPSNNDNPDALEALPVVRQLIFEGKYDE
                     AQNLVNQKLTAKQLHGSMFQPVGNLLLTFGHTNFTDYYRELDIEKAVFTASYKVDGVI
                     YKREVFASAPDQVIVLRLTADKPGKLSFVAGINGPLQKTMTVLDNTTLELTGLSSDHE
                     GVTGQVKFNARVKLQLTGGAAEALPDGIKVSNANEALILISVATNFVDYKTLTANETE
                     KSANYLSKAEKKTFAELYKKHVAAYQQYFNRVSIDLGSSAARALPTDERVRNFSQTND
                     PELVALYYQFGRYLLISSSQPGGQAANLQGIWNDQVHPAWDSKYTININTEMNYWPAE
                     KCNMTEMHEPLIQMVRELSESGQNTAKTMYGCKGWVAHHNTDIWRICGVVDGSFWGMW
                     PMGGAWLSQHLWERYLYNGDLKYLESVYPVLKSACEFYQDFLIEEPVHKWLVVSPSIS
                     PENAPAGRSASVNAGNTMDNQILFDLFSKTIKAARLLNKDNALIADLQKTLDRLPPMQ
                     IGQYGQLQEWMQDLDNPKDEHRHVSHLYGLFPSNQISPYSSPQLFDAARNTLKYRGDV
                     STGWSMGWKVNFWARLLDGNHAKKLITDQLTLVDPVRPASGGTYPNLFDAHPPFQIDG
                     NFGCTSGITEMLMQTHDGAIHILPALPDDWKTGEIKGLRAYGGFEVSFSWENGKVKEI
                     VIKSNLGGNCRIRVPNKVQLAGGLKLKVAEGVNPNPFFAVPQIKSPIISDVAKLSAVE
                     LKPTLLYDFETKAGQKYIIHLLKE"
     gene            complement(39062..40981)
                     /locus_tag="PK252_07490"
     CDS             complement(39062..40981)
                     /locus_tag="PK252_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14589.1"
                     /translation="MKKLILSLSAILALGIVSIAQVAEKHAPQGFDVVRTNIAHGKID
                     TITYQSKTVGVARKALIYTPPGFSKSKKYPVLYLLHGIGGDEKEWFTQGQPHVILDNL
                     YADKKLEPMIVVLPNGRAMKDDRAVGNIFEPEKVQAFATFEKDLLNDLIPFIEKKYPV
                     IKDKGHRAIAGLSMGGGQSLNFGLGNLDVFSWVGGFSSAPNTREPEQLMPNPADAKKK
                     LKLLWISCGDNDGLITFSQRTHDYMVKNDVPHIYYIEPGVHDFKVWKNSLYMFSQLLF
                     KPVNVANFNKYSVLGSPSSYNVRGAKYPQVLPDGRAVFRIKAPEAQKVQVDLFRKYDM
                     VKGEDGVWEVTTDSLSEGFHYYTLIIDGVSVADPASETFYGMGRMASGIEVPFKGDGY
                     YAIKNVPHGEISIKRYFSNVTRSWRNLYIYKPAGYDQNTSEKYPVLYLLHGGGEDERG
                     WATQGKTDLILDNLIAEKKAKPMLVVMMDGNFNSQAFGEEVLKTFEAELKLSVIPFVE
                     KNYRAETDAQHRALAGLSMGGLQTLYAGVNNYSLFAYLGVFSSGWIVPMQADLANKQY
                     DFMKANADAINSNVKQFWVSQGGKEDIAWNNCQVMIKKFDELKIKYTYSEYPGGHTWP
                     VWRNNLYNFAQLLFK"
BASE COUNT        12497 a         8861 c         7280 g        12435 t
ORIGIN      
        1 agccaaattt gtttaatttt taataacgtc ccgattttaa tgggacacta gtgacgaaat
       61 ctatgaaatc agggggaaca aaatgcccat aggcgtttac atcaatgaca acaaaccttt
      121 tacgtcaaaa aaaattgagt tgcagcccgc tgacaggttt tacatcacca ccgatggcta
      181 tgccgaccag tttggcggcg aaaaagattc aaaatttatg atgaaaaggt taaaaaagct
      241 attgcttgac aatcacaaac tgccatttaa caagcaatgt gaaattcttg aacatacttc
      301 aaataattgg cggggcgaat gctaccaggt ggacgatatg tgtgtgttgg ggtttgaatt
      361 ttaaaaaggc acagcctctg ccttttttgg cagaaaccgt gcccaacttt ttagcacaaa
      421 agcttacagc tcagccttta ctaactaatt ggcgatctgc ttcttccagc tcttttcttt
      481 tcttttcctg aaaatcaact accttctttt gaatagcagg atttgacaac ccaaatattt
      541 tagcagcagc caaagcagca ttttcaggac taagtatcgt cataggcgct acacccgaag
      601 gcatacggat acttgaaaac acatcggccc caccaaactt gtcactataa ggcggacaag
      661 ctataaccgg gcaacatgtc tgggcatcgg caaaaccact cagggcgttg ctcatcccag
      721 caatggtgat aaatactaca ttttcctttt catattcttt aatgaggtca tagcatttca
      781 acggcacctt gtgagccgaa gcaatacggc aaacactggc aacatcaaaa ctatcaagca
      841 cctttacaat ctggtttgcc cagtcgaggt ctgccttgga ccccatgata ataacaactt
      901 tactcatttt cactgttttt aaagaatgaa tattgatagt ccattggaat aatagacagg
      961 tattcttcaa cgcttgcacc agccaggcaa gcttcaatta tctttcggcc ggtattggac
     1021 aattccgggg tcaagtattg ttgcaactcc tttcggaaaa agtcatacaa gatttcggcc
     1081 ccggcatcgt accccgaagt ttcaacctca acctgcttgt gtacctgtaa gaaccttgaa
     1141 ggaatcttgg ctccttcaat agtcaggtaa ttcagttcaa agcctaacaa tggacagcga
     1201 gcatactggt attggtcact tcttaactta gcattaccac gtcgggtaag gtattcgcgc
     1261 ataagcagct gaggcttgaa acccaccttc caggctccga tatgttggtt agggacaagg
     1321 gtataacgca tacgtggagt attgataatc tgttcgagca gcaaattggc atgagttatc
     1381 atttttcctg ttgcaaaagg ccaatacgag ccaacacctt cactacccat acctgaagca
     1441 tctacgatac ttggattggc atgtcccctt ggggctacta acctccacaa ccaagccaaa
     1501 gcagggggca atatatgaaa caacccaata ataccataag tagggttttc tttggtacag
     1561 ggcggggtac gcaccccaaa acttctcaca tcaacagtta caggcttacc cacaacattg
     1621 ggaacaatgt tcctgggaac aataaccctt gggttaggac attttttacc agggctgtct
     1681 tctgtatggt cccaaataag cgcagtacat tcgggattcg acttaatatt gaggaacaac
     1741 aatggttctt taggcttgat agttagtttt tcaagaaaag ggtcatctcc ataatcattg
     1801 atactgtcca cccttataaa ccaagcattc tcggcatcga gcactgttag tttaccatta
     1861 ttcttttgta ttgaagggtg acaaagtgcc atatcatccg taaccgggtt gaacgagcag
     1921 aataacggaa agttaatcag cctcttttcg ccagtgacgg tattttcacc aatcaacacc
     1981 tgaccattct cttccctgac aatattttgg agcatttcac ttttgccccc accgctggca
     2041 ccctcatgca taaacgtggt aatattgtca taaggactaa ccacctgcac cgtagaacag
     2101 tgagctgtta cccagccttc tctttcgcct tttgtcagca aggctccgta caaacccttc
     2161 ttggcactgg gcccggggta gaggttgtaa gaaaatattt cgtgcaaccc ttccgacctc
     2221 ttgtgtacaa caatttgctt accggcaaaa tgggtatgtc taaaagttgg agcaatataa
     2281 atggctgatt ctattgcaaa ttcttccgga agatcattca cagggataat gctttgaagc
     2341 atagcaaggc ccatggcaaa aaaaccggca ttagctggag ctatggcaat accacccgaa
     2401 cctatttttt cgcgaccagc aaaataaaaa aacactgcca aatcttgctt ttgaagccat
     2461 tctatagtct ctttccgcaa aacatcaaaa ctataaccaa agcggtcagc gaaacgctct
     2521 ttatcactgg gataatcatc agcaataacc attgtatcag ggtcacggcg acgcatgtac
     2581 gcctcggtat aattgaccga tatcccgttg gttaccctgt gaacaatcgc ctccgtcatc
     2641 tctcctttcc cttctatctg gtacttgact tcgtaactgg cctggtcgcc cccaacagca
     2701 gcagaagcca actctttaac tgtatcaaag gttttataag atttgcaacc actcagcata
     2761 ataagtgcat cgtcaggtaa gctaatcctt ttcttttcca gttttttaaa caaatcttcc
     2821 ataatatttg aatttagcaa cgattattaa caattgtaag tttttcaaag atatatattt
     2881 ttacttaaca aatgaagtaa tacttaattt atttgacaaa aaatttttaa aaatttggag
     2941 gcgattggac ccacagtaat tcactaagcc catcattact attaatagca ttaaaagata
     3001 tttttgcatt aaaataaata ttatcgccct gtttgaggtt atagctttta tctgccaggt
     3061 caaacctgat ttcacccgaa aggacatgac aaaaaacctg tccatgctta gttttaaaca
     3121 agccatcaat gccagaagct ttggcaaacc gcacaagata agtatccata agcttgttgg
     3181 gatcatggtt tgataacaaa aaaacgatag tgccagtgtt attctggtca acaaacttaa
     3241 tttcactttt tgaaaccaca ggatgattgg ccattgaatc attctcgccg accaaatcgc
     3301 caatagtagt atgcaacgca tcggcaatgg ctttcagcgt cataatagac ggaaacgact
     3361 ttgatttctc aatttgcgaa agagcactgg gactaatatc aactttttcg gccagttcgt
     3421 taaggtgcag cttcaaaagc tccctttttt tctttattct ttctcctatt aagttcatac
     3481 acaataagtt tttggcaaat atacaaaata attttataaa ttaagtaata cttaatttat
     3541 aaacaatacc cattacagct ttacgtttat atcaaaatca atatccccaa aagccgagtc
     3601 ggtatgtatc cctttatctt cttacaacga atatatgtta ttgggacaaa aagcacatca
     3661 aacaaagtaa tttggtcaaa gcaaaaaagt ttttatcaaa aaaaattaag ttttttacat
     3721 ttataaacaa catttctgca acaaaagtgc ttatttattt aaaaatatag caaccattat
     3781 tattccaatc aaagaaaaag tgctaaaaat tattattatc tacactttac aaaaaattaa
     3841 ccagtcagtt aagaaaaata tttttgctat cactaaattt atttagtatt tttgtgcaat
     3901 ataagttaaa atacaacagt ataaaatatg aaaattgtcg ataatttaaa tttagaccag
     3961 gcaactaaag aaaagctttt ccagagcgag gaggcttgct ttgccatgtt agccgggatg
     4021 aaatggaccg aaggttacat ctgcaagaaa tgcggccacg acaatttttg tgatggcaaa
     4081 acgcctcatt caaggcgttg tacacgctgt aaaaacgaag agtctgctac tgctcacacg
     4141 ttatttcata atataaaatt tcccatcacc aaggcatttt atattctttt tgaagtagcc
     4201 aacaaccaca aatactcgat atttgacatt gctacccaaa taaacgtcag acaacttgtt
     4261 tgctggaact ttaaaaataa gattgaagaa aaatacaaca ggcttgtgcg gttcaacaat
     4321 attgaagcag taacatttaa cgatttgatt atcaaagaag caaattcacc ttttgcttga
     4381 gccatccctc attaaaaaag ggatggctca ataggtttcc cgctgcttgt atttttaaaa
     4441 atgtcttctt ctttaatatc tgcaattccg ctttgtaatt cttgcgattc atcgggtttt
     4501 acaggtattt ctttctgcag aggttcaatt tcggcgacag tttttacttc aaaagttgta
     4561 agccttcgtc ctttggccct aaagcttttt actcctataa actgttccac atcgataatt
     4621 tcggcaggac gatttttgtg tttgccccca aaatcaatct taaactgagg aaaaaactct
     4681 gtactcagca atatcagttt tgattttgga ttatcatcga taaaattctg aggtctttca
     4741 gacatttcaa aggtgcatcg ttttacataa taaaattgtt gctctgcatc aaaatatact
     4801 attgaaaaaa ccttatctgg tgtatatttt tcgataaaaa caatatcatc gtcaaaatgg
     4861 ttggtcaact catacgaggt ggtataaaac tcgccttttt ggtttaggac caaaatcttg
     4921 tcatttccga taaattggcc caaataagtg ccacgttcat cggtattgag cctgaaagta
     4981 tcattatcaa accagatggc agttcctcct attgtagaaa tacctttttc tttcagttca
     5041 attttatgga tagcatgttt ggtaaggata ttaccaatag ctccacgtcc tttaacagct
     5101 aattcggcaa aattaaaaat caaatcgagc accttaaggc gcggacgggg ctttagcttc
     5161 accttaacag tttcagcctc accattaggg tttaccgtga ggtataatat ttttgaataa
     5221 ggtgtcccct gggtcaggtt atattctttg tcgcgggtga gaccattgat agcacagcgt
     5281 tttacatacg aaaaacccga ttcgccatca aaatatatga tgttaaaaat ggtgcgggta
     5341 tcattttttt taaataccga aacgtgcaaa atatctttac ccacaaaagt tttttcagcc
     5401 actttagtag tcaggtaagt tccatcacgg cgaataacca gtatatcgtc aatatcagaa
     5461 caatcgcaaa cgaactcgtc ttttttgagg tttgtaccaa tgaaaccgtc tgccctgttg
     5521 atgtaaagtt tttcattatt ggcaataaca gcagtagctt caatactttc aaagctacgc
     5581 aactcggtaa gcctttgttt gtcggctccg tacttttttt taaggttctt aaaccaatta
     5641 atggtatatg ttacaatatt atcaagatca tactgcacct gttccatttc tttttcaagg
     5701 cctttaatca gctcatcggc cttctttatg tcgaaacgcg aaatgcgcct tacaggtttc
     5761 tcggtcaatt taagcaagtc ttcacgggta atctcacgtt taaagcgggg cctgaaaggg
     5821 ttaaaggcct tatctattgc atctatagta agttcccatg attcggtttc tttttcgagc
     5881 tctcgataga tcttttcttc aaagaaaatc ttttcgagtg aagaaagatg ccaatcttcg
     5941 agcaattcgg ccattcggat attgagttcc tgacgaagaa gctctaaagt ccggtatgta
     6001 ttggccctta atatttcact tacgccaata aagcggggtt tatcattatc aatgacgcaa
     6061 gagtttggcg aaatagagat ttcacaatcg gtaaatgcat ataatgcatc aatggtaaca
     6121 tctggcgaaa ctccaggctg taaatgaaca ataatctcaa ctttttcagc ggtattatca
     6181 tcaatcttac gtatttttat ctttccttta tcatttgcct tgaggatgct atcaataagg
     6241 gttgaggtgg ttttcccgaa agggatgtca ttaatgacaa gggttttctt gtcgagctta
     6301 ttgatttttg ccctaacttt aacaactcca ccacgaaggc cgtcattata ctttgaaata
     6361 tcagccaatc ctcctgtggg gaagtccggg tacaaggtaa atggttcatt tttcaataca
     6421 gcaatagaag cgtcaagcaa ttcattgaag ttgtgaggaa ggatttttga agccaggcct
     6481 acggcaatac cttcaacacc ctgagccagt aataaaggga attttactgg caaagtgaca
     6541 ggttctttat ttcggccatc gtacgaaagc ttccattctg ttgttttagg gttaaaaata
     6601 acttccatgg caaactttga gagccgagcc tcaatgtaac gcggagctgc agccgagtcg
     6661 ccggtaaaaa tattccccca gtttccctga cattcgacag ccagttcttt ctggcccagc
     6721 tgaacaaggg cttcgccaat agatgcgtca ccgtgtgggt gaaactgcat agtatggcca
     6781 ataatattag ctactttgtt gaaacgtccg tcatctaaac ggcgcataga gtggaatata
     6841 cgtcgttgta ccggttttaa accatcatta atgtgtggta cagccctttc gaggattaca
     6901 taagatgcat aatctaaaaa ccaatccttg tacattccgc caagaactgt agtatgaatt
     6961 accttgtcct gaaagtgcga accaggggca gcctcagcag aagtttcgtc tggcaggttt
     7021 tcgagctcct ctggatccgg tatattatct aaatcgtctg acatgggtca attactaatt
     7081 acgaatgact aatgaatatt aagtgctttc tcattttcaa attggctaat tggctaattc
     7141 taaaaggtct tcttcaaacc ttaggttgtc aatgataaag tcctggcgct cctgggtatt
     7201 cttgcccata taaaactcaa gtaaaccatg aattacatcg tctttacgta aacgtacagg
     7261 gtcaagccta atatctttac caatgaaatg cttaaattcg tcgggagaaa tttcaccaag
     7321 gcctttaaac ctggttatct caggatttgc cccaagagac ttgacagcct tttccttttc
     7381 tttttcatca tagcaataga aagtcttttg tttgttacgc actctaaaaa gcggtgtttg
     7441 caatatatac aagtggcctt tacgtattaa atcagggaaa aactgcaaaa agaacgtaat
     7501 aagcaacaga cggatatgca ttccgtccac atcagcatca gtggcaacta caatattgtt
     7561 ataacgcagg ttttcgtaat cttcttcaat atttagggca gcctggagca ggttaaactc
     7621 ttcgttttcg taaacaattt tttttgtcag cccgtaagta ttcaggggct ttcccctaag
     7681 gctaaatact gcctgggtgt taacatcccg gcttttggta atagaaccgc tggctgaatc
     7741 accttcggtg ataaacaagg tagtttcaag cctgcgttcg tctttatcgg taagatggat
     7801 acggcaatcg cgcaactttt tattgtggag gttggctttt ttggcccttt ctttggccaa
     7861 cttttgtact ccgctgatag ctttacgttc tttttcattt tcgacaatct ttttttgcaa
     7921 cgcttcagct atgctggcat ttttatgcaa gaagttgtcc aaatcgcgtt tgagaaagtc
     7981 tgtaatgtat ttgtttaccg aaactcctcc ttcaaccata tctttagagc caagcttagt
     8041 tttagtctgc gactcaaata cgggttcctg gatacgaata gcaattgcgg ccacaataga
     8101 cccgcgtata tcagatggtt cgtattcttt tttataaaac tcacggattg tttttacata
     8161 cgcttcttta aaagcctgaa ggtgtgttcc tccctgggtg gtatgctgtc cgtttacaaa
     8221 gctgaaataa tcttcgccat attcattgtt gtgcgtaata gccacctcta tatcttcagc
     8281 ttttaagtga ataacagggt acaagccttc ttcagacatg ttatcggtaa gcaggtcaag
     8341 taacccgttt ttcgatgtaa atacttgtcc gttaaactta attaccaatc cggcattgag
     8401 atatgtataa ttgcgcaaca tagtctcaat gtattcttga ataaaatgat aattgccaaa
     8461 caatgcagga tcgggcttaa acgtaacgcg ggtgccattt ttctcgctgg tggttgtttc
     8521 aggaaattcc tctactttgt taccagcgtt gaatacaact tttttagaaa tattttcgcg
     8581 aaaagactca atagtaaagc tttcagaaag tgcattaaca gccttaaccc ctactccgtt
     8641 caaaccaaca gatttttgga atgttttaga atcgtactta gcgccggtgt tcatacgcga
     8701 aactacttct acaagtttgc ccaacggaat accacgccca aagtcacgaa caacaacttc
     8761 attgtcgata acatcaatca caatctcttt tccatagccc atcatatact catcgataga
     8821 attatcaatg acttctttga taagaatata tatcccatca tctggagaag agccatcgcc
     8881 aagtttacca atgtacatac ccggacggcg gcggatgtgc tcgttccact caagggtttt
     8941 aatgctatct tcagaataat taacttgttc catggtggca gtttagtatt gtattatgct
     9001 tcaaggcctt gcaatgcaag tgttaacctc gtctcaaagc acacaaatat agggaaaagg
     9061 tttcaggaaa aagtcaattt tttatcaact atattgttag agcccttacc aaacaataaa
     9121 aataatttat caacaaatga aaagctactt ttgaagggca attacgttat agaccacgtt
     9181 cttatccttg taataatcat cacggtcaaa tttccattgc caagattcag caactggcag
     9241 cgaattgaat gacacctgca gtaaaggcct ttgtttttca agaatattat cttttgaata
     9301 caccaactcc tcgtaatctg taataagaaa agttttacca accggaagtg attgcaaatg
     9361 gctttcttca actttacgac tgagtaaggc caattcttcg tcagaatata ggccatacgg
     9421 agtgatatat tctaccagta aagtagccag ctggctaata atattggcag aaataacaaa
     9481 atcaaactct tcgggatatt gaaaaccttc aaattggtat gtatcaatat ttttaacgta
     9541 tttggtactt ttgtaatact gtacagcttc aaacatctga gttatcacgc taccggtaat
     9601 atccaactca acaaaaacaa cctttggatt gtgtgcatac ttatgtttaa cctgcgtagg
     9661 atgtatgata tcaaaaagat acactttttc aaaatgctct aacaatgcat caatagggat
     9721 atctaaaagc caaccactac ctaaaacagc acatttgccc cttcctcttc ctgataaatc
     9781 cttttttata gctgctttac aatgctcaag atggctgccc caattttcag actcttttaa
     9841 aaatcttttt attatccctt gctgatcagc tacataatgc atttttttga gaatacggta
     9901 ttttttattg aaaaacattg gttgaagaat ttagagtcaa aattcaaaaa tcaagacgag
     9961 aatcaaatat taattttctg agcgcaaagc agccaattct ttggctatca tatccctgaa
    10021 gtattctttc agttcggccg ggttgtaagt tggaaattta tcgtatggta cttcatcaag
    10081 cacttttaca ataatcctat cacccttatt tacggctata ccttctttag gaatagcatt
    10141 accagtacca tccaacacaa ttggaacaat agaggcctta ttttcaatag ctgccctgaa
    10201 tgctccatct ttaaaacggc caatttcaca gtttttcgaa cgtgtccctt caggaaacat
    10261 cataatggtg aaattcttag ccaatttttc ggagatcttt ttgtacattt ttgggaaagt
    10321 cttaggatcg tttctatcaa ggcttatata accattaagg tacataaccc atccaagaat
    10381 aggcgcttga aaattttctt tctttgaaac ccatatataa taagggaata cagcaaatag
    10441 cacaagtata tctgccatag actgatggtt tgatatagct acagctttca tacaaggctt
    10501 taacttatgt ttgccaatta cttctacacg cttaatgggc agaatcttca aataaagcat
    10561 agcccacaaa ctggtataat acatattaca acggtgccgc tgatcaaaag gccagcttat
    10621 aatccaaaga acaaaaaatg gaataaacaa caccactaat gacacaacgc caaaaaacca
    10681 tatccaggca gtagtcaata acttacgcat agcgactcct ttagttttta aagcgctaaa
    10741 attaggtttt taaacccaaa gaaggggtgt aaaaaagcaa aaaaaaagca aaccgcaaag
    10801 gtcacaaagt tttttgcaaa gaattcaaag tgataataat caacacttta aataattaca
    10861 tattttattc tttgttcgtg tactttgtgg ttaatatttt tttacacacc ttcataaatt
    10921 ggcctgatag ataaaaggtc caggtctttc ccagacttta tggggtatat caacaaggta
    10981 tcacttagct tctacttcaa taaccttaat ctgtttccac cttccacctt gaaaacgcaa
    11041 gtacagcacc ccgcaaaaaa ccataaaaat aattaccatc aaaaaccagc ttaatacaac
    11101 agaaatattg aaaacatgaa gcgaaaggta taaaataggc actaacaccc agtgggcagt
    11161 aacagaagca atcatggtaa acatggtatc tcctgcacct cgcaacgccc caatgatagc
    11221 aaccattata gcttctgcaa gtacatatat ggcagctatc cttatcatag caactgcaat
    11281 tggcacagtt tcttcaaaac ctgaagacag cccctcggga ctaaagaccc ttaccagcga
    11341 ttcggggata agcaaaaaca agaacaatat gacaatggaa taaaaaaaac cgcttttgac
    11401 acctgacaac gctgcacgat gcgcaacctg aggcctgcct gcacccatat atcttccaac
    11461 gaggctggta acagctattt caattcccag cagcggaata aacgacacca aatcccagtt
    11521 aaacatgatg gtagatgcag tagcaataac ttctcctttt gaatgaaaaa ccgtaaccag
    11581 cattgtcatg gcgcagaaaa gaagaaacaa ctcaagcccg gcaggatagc caaatttaat
    11641 gagcttcttc atgatatccc aatcgaaatg aaaagattta aaaacagaaa actcaacaac
    11701 atttttcttt tggaaataag ttagcaccag tattatcaaa gcccccagag ctccacaaac
    11761 agttgctata gcagcgcctt ggatccccaa agaaggaaag ccaaacttcc cgaatatcaa
    11821 caggtaatcc agaatgacat tgataactaa agcagctaag gtagccttca tcactatctg
    11881 agttttacca ataccactaa aatagcaccc taaggtataa cgcaacaagc caaacaaacc
    11941 tccccaggcc aaaatattta aatactggac ctgatacccc aactgcacct gagaaatatc
    12001 agaaaagtga taaaacgaaa ccaccaaagg cttcaaaata agaataacag gccaagccag
    12061 gacagtaatc aaaatagcct gaaaagcagc ttttgaagaa ttatgttttt cgccggcccc
    12121 aaaatactga gcaacaagag cagtagaata cccggtaagc ccaacaaaga aaaacatcaa
    12181 catttgcatt gtcactccgc ctcccagagc ggcattcatc tgctcagaac caactttagc
    12241 aagaaacaag cggtcggtaa aagtcatagc cccgtcgcag gctgtagaga caatcatagg
    12301 aagggcaata gccaacagtt cctttattcc tcctagtttg tgataacccg agaaataatt
    12361 tttaaaaaaa ttaggcataa ctatgtaaaa attaaaaaag taagagatta gataaacaat
    12421 aacaccaatg cagcctttat ttgaaaaagc cacattagaa aatgtataaa aacaaacatg
    12481 gaattttgga ttaagctttc atgcagcaaa attaggctta agcagattaa caacaaactt
    12541 ttttacaaaa aaaatgacat ttctgctcac agatattgaa ttgaaacaaa gcaaataaaa
    12601 aagccctaat ggctatgacc ggttgacatc gccatcatca aaacaatacc ggcagaaata
    12661 gcgataagct gcaaaacaaa tgtacgaaaa ggttgtttgt gaagctcagg aataaggtcg
    12721 ctacatgcaa ggtaaataaa accgcctgct gcaaaaggta agatatatgg aataatatca
    12781 cgtgcagatt gtccaacaag caaagtaata acagccccaa caatagcagt acatgccgaa
    12841 aacaagttaa acatcaaggc cttacgtttt ttgaaccctg catgtataag aatgctaaag
    12901 ttgctaattt cctgtgggat ttcatgaagc ataacagcaa tggttgtagc cataccaatt
    12961 tcattgcttg tcaaccacga tgccgctata agcacaccat cggtaaagtt atgaatactt
    13021 tcggcaatta gtgtcacata cccaaacgaa tgctcatggc cacagggatt ttcttcaagg
    13081 ttatgctgat ggtgccagtg aaaaaccctt tcggtaataa aaaagagcaa taaaccagct
    13141 gctaaagcca agcccataat gttttcagaa gcatgctctt caaatatctc tggcaacaag
    13201 tgaaaaataa cattgcccaa taatgctcca acagcaaaac taaccattat aaacattatc
    13261 tgtttcaaaa catcatgttt taaaacaaaa aacacaatgc caatacctgc tattgcacta
    13321 actgcaaaag ttgccaccaa tgcatttacc cagactccat tcatgactat ttattaatta
    13381 ggttaaaagt caaagaaacg gcaaacttac agaaaaagta acattctgct acaaaagaat
    13441 attcttgacc aaaatattag actcagattt ctccatggcc ttattcattt tcagtacaat
    13501 atattcgtat ggtttagatt ttaaggcctc tgctacaata tcgtttttca ctcctttagt
    13561 attaacagcc tcaattacaa aacctatcgt aataagcata ggatcaacag caggttgata
    13621 tgcctgttct tcgtctaaat cagtataaac ttggattagc acccccgaat caaccaactc
    13681 ggtaattaaa tcggcaataa gaaagttagg cagctttgtt tttattgcta tttcggcaat
    13741 agatggagct ttttcacctt tagcatattt atcaacaata acatgcgtta tcataaggta
    13801 cagggccctt ttggtgtttg tactaagctc tgtaagacgt atattggtat attcctgctc
    13861 aatattgtga gcaatataac aaaattcagc gccaaaaagt actataagcc aacctgtctg
    13921 tacccaaacc ataaacaagg gcaaagcagc caagctccca tacaaagcgt tataacccga
    13981 tacgccaatc tgaaacttga cataagccca ctgagtaagc tgaaacatgg tacctgaaag
    14041 tatccccgca acaattgccg ggccaaacct gactttggta ttgggcactg tcataaacac
    14101 catagtaaac aaggcccaga caaggatata agggattaga gaaataaaaa acttgataaa
    14161 tggggcaaaa aaacccaaaa atgcaatact tgaaatgata gtttttatgt gggtggtaag
    14221 atagacagtt gcagagctgg agaggattat aaaaattggg gcaaagaaaa taagcgcaaa
    14281 atagcccgaa agtttgcggg taaatttccg gccttttttt atgtgccaaa tgtcgttaaa
    14341 agcattttca atattattga gcatacgtat tacagcccaa aacaaaataa taaaacccac
    14401 gccggcaatc cactctccac gggccttgga aaatatagac ttagcaaggt taattaccca
    14461 caaggccact tcctgctgag ctgcaagttt gttcataatt tcgcgctgca gcatccgctc
    14521 cataccaaac cctttggcaa taccaaacac caaggccaaa attggcacca tggacaataa
    14581 ggtaaaataa gtcagcgaag cagccctcat ggtagattta taggcaaaga aacctcgcga
    14641 agcaagcatt gcaacacggc cgttttgttt taaaaaaaca gctacttttc ttacagtatt
    14701 agaaatctta gtatttttca atgttaaaat tttcatgttg aagttaaagt aaaactatca
    14761 caaaaacaaa ctttcaatat cttcacgttg catggctttg aagggattgt tagaatttat
    14821 gaaagctttg gccataccag acttgtgttc ctgaagttta aggatctttt cttctaccga
    14881 ttttgttgta atgaagcggt aaacaaacac cttcttgtcc tgcccgatgc gatgggcgcg
    14941 gcttatagcc tgattttcgg ctgccgggtt ccaccaagga tcgataatga aaacataatc
    15001 ggctgaggta aggttgagcc ccaccccacc ggctttcaga cttatgagaa aaatattatt
    15061 ttcttcatca ccctgaaaac ggtcaattac ctgcttacgg tcgtgcgtag ccccggtcag
    15121 catggaatat ttataaccgt tatgctcaag atatccggca ataaggttaa ggtgctttac
    15181 atacgatgaa aagataagta ccttatggtt ttccacaatt acattctcta tactgcggat
    15241 aatttcgtca tatttaccag aaccaccttg gtaagacgca tcaacaaggc agggatggtt
    15301 tgacataagc ctcaaacggt tcaatgcttt gagaatgaca atggccgact tttcaaagcc
    15361 atgctggtca atgttgttca taatcatgtt acgcaccgat gacttttcct gctcgtaaag
    15421 ctgagcttgc tcctcatcca tctcgcaaaa aattgtttgt tcggtaagcg gggggagctc
    15481 ttttaccact tcttctttct tgcggcgcag aataaacggg ttaatgagca ttttcagctt
    15541 ttgttgtttg gcttggttgc catatttttc gataggaata acaaattcct cgcgaaaata
    15601 attttgactt ttgagtaatt cgcggtttag gaagttaagc tgcgaccaaa ggtcggtgag
    15661 gctattctct attggcgtac cagtaagtac caacctgtat tctgattgaa gtctaaggat
    15721 agccttgtat atcttcgaat ctggattttt aatcatctgg ctttcgtcca ggataacgta
    15781 tttgaacatc agcttagaca agtggtcaat atcgtttcgc actacgccat aggacattag
    15841 tacaatatct gcacgcaaaa gttcttcata agccttcatc cgctgcgagc caaaaaactt
    15901 gaccacatgc aacgatgggg caaacttttt aaactctgtt tcccagttgt gaagcaacga
    15961 aactggcata acaatcaacg aagccccgat acacctgctt cctgtaaagt cgggattatc
    16021 aaacaagctt aactgtcttg gccctgtccg ggtaattgtt tgagctattg ccggattgcg
    16081 caactcctct tccctaacct tcaaaagcag cgctatggtt tgcaaagtct ttccaagacc
    16141 catatcatcg gccaagcatc cgccaagctt atttttataa agctgaaaca tccattttac
    16201 accggtcttt tgatattgtc gcagtttagc ctgcaatcct tgtggctctt taagatattt
    16261 attttccgaa aggctcagtt tttcttggat acccattatc atttgcctgg tttcaacacc
    16321 cattgattct ttcaggatgt tgaaatggtg tttttttagc ctgatgcgtt catcatcgtc
    16381 ttctccaaat acaaacaact cgcgatacct ggaaaaccag gctaacggta aaattactat
    16441 ctgattgtta ggcaaagtaa actctctgat attatgcaca atatgtttgc gaaaacgaga
    16501 aaaaggaaaa acatattcgc caatagtaac cacggcattg atatcaaacc aatcgtgtgc
    16561 ctcgttgaca ttgagcttca gcagcacctc atgcaggtaa taattcttat cgaacttcat
    16621 ttgatccagc tcaaagcctt catcctgaag catgcgggta ttactgttaa tccaacaaac
    16681 catctcgtaa aactgccatt caggctcacc ttcaagcgca tgaagcacaa aagcccctct
    16741 gtcaatatac ctgaagccaa aagaaaccag tttatctttc atcgactctt cccaagccaa
    16801 gtcgcgcaag gtcttgtaaa agcaaacctt atcatcggca tctgtaagcc cgacatgccc
    16861 gataacatcc gagccatatt caaatttcac ctcaccgtaa aagaattgca gcataaatac
    16921 aggctgaccg gacaatccag aatcgagcac cagcatagct ttctttttgt agtctgttgt
    16981 ttcaaccctg aaaccgacta cttttacggg gtgatgcaaa atggcatttc gtacaaaagt
    17041 ttcgagatag ctcttttcgc tcgtctttgg caccttaatg aagtctttgc tgaaaaacgg
    17101 gataagcttc ttagaatcta tctgactaac ctggtaaagt acattttcga ccaccacaat
    17161 agccggttgg ttggacaaaa caaagtacga ttttccaaaa agcttgatat ctttatcttc
    17221 acagcggaga ctaaggaaat atttaaagtc aacatcggtc ctgacaaaat taaaaaccgg
    17281 atgtaccaat gaagacgtaa gttttatctg ttcatcatca tgaacaacgt tgtatttttg
    17341 gtctttctta tataccttct taccaaaagc aaccattaac tcaacgcaag aaatgatacg
    17401 cttttcaata taaggcctga tatgttcttc aactaaattc tgatccaatg tatcgaaaaa
    17461 ctctttaagg ctgttacgct tagcaaagtt tttatggata tttttttcag aataacctga
    17521 ggtaattttt acaagctgga ggtcggtttc atccaggata tttttgtaag gctcaatatt
    17581 atcaacagtt aaatactcaa ctaattgaaa aaaagattgt tgtgctttac gttcgataat
    17641 ataaggcaca acaaggtatc caagttttct gtgtaaagtt aatatgtaaa ctacttcgag
    17701 cttcatcagg aaaaaattag gcaattgtac aaaaattttg ccctaaaacg gttctgctaa
    17761 gccccaaagt tgttaacaat acattttaaa acacaaagac acaacaacac aaagacattg
    17821 ttttcttaag acctttgtgt ttttcaattt gttctgtatt tgaattttag cactaattgt
    17881 acaattccta ttcctaaagc aataatatta caaagaatta caggcatagc atgaatgagc
    17941 agcccataaa cctcccataa agtacaattg ataacataaa cgacaagcat actcatagaa
    18001 atatcgtcca cacgctttgt ttttattgac ttatacactt gaggcaacat caacgctgtt
    18061 ccaaacactg cggccaagta tccaacaatt tctgtaaaaa gctccatttt ttatttaatt
    18121 aaaatttaac cacccctacc ctcccgcctg aggcgggaca agctctcctt ataaaggagg
    18181 agaaatactt cctatttttg agaacgttag taactaaata cacgcataat acaactctat
    18241 ctatcaattc ctgattcttg gctcttgact cttgcttcta aaatctactt atgacaaatc
    18301 tgcttataat cgcagtattc acaagtcttt ttagcagtag tctgcacaaa aggtttctcc
    18361 acattccaaa tatcttttaa aaggccaaga agattctctt caaaagtagc caataatggc
    18421 tgaaaatcag taatggtctg tcggttcatg ctgaagtatg tttgcccgct agtatcaaac
    18481 ttacttcgaa gggcataaag acatggcaca ggaacagcag gcaaaccgcc ttgtttagaa
    18541 gcaatcaaag cgtaaagccc ggtctgaaaa atctctttct tactattttc ggtcactccg
    18601 gctgcaaaaa tatctttcac ttccgtgaaa gacttagcca cattgcccgt tttataatcg
    18661 acaatacgga tgatatgctg aacctcgtca atacggtcta ttttgccgcc tatctttatt
    18721 tccttctttt ccgggccaat gtttacaata aatggctgaa caacttcctg ctctaatgcc
    18781 atgatatgta ccggagcaat ttccttgtcc atttccagca cccgtattac atagcgttgg
    18841 attacctctt taataataac gacctttcca tccatcatgc gctcttcatc tttcaattct
    18901 tccaaaaggg cagcatgaat aagctcctga atccttttct tatcagctat cagcaggtct
    18961 attatttcag ccgaaagcaa ttgaccaata tatggtttgt ataaatgttg cattacagca
    19021 tgcacaacca atccaatcaa agccccttct acatcctcag tcacttcttc ttctggcttt
    19081 aagcgcttaa tgtatttata gcaaaacttg aggggacaat caatataagt acttaaagca
    19141 cttggtgaaa aatacttttt ctggacaata tcatctaaag ctgcaattac agcagtatct
    19201 ttagcaactt gaattggctc tacccggcca ggagtgatag caaaagactg gcttttatac
    19261 tgcaaatcca ttttatgtaa atactgcaat tgcaaggcat agcggctaat ctctgaagca
    19321 cttcgactat ccggtgcgct attgtacagc aaagtaacat tctttgccct atgcagcaaa
    19381 cgatagaaat agtaggcata catggcatcc ctgtatttca tggtaggcag cttaaaaccc
    19441 cttcgtaagc tgtatgggat aaacgacaca gggaaattat tcttaggcag cttaccttca
    19501 ttgaaaccca ggatgacaac attctcaaag tcaagtgcac gggattccag aaaacccatc
    19561 acctgcaatc ctctcagagg ttcgccttca aaaggtatct gcacagaacg taatgctttc
    19621 atcgtagccc tgatggctac ttccagactt atcaactcct cccctatcaa cgtcaactgt
    19681 tgctgcaaaa ggtcaaccac cggaagaata gacgcaattg attgttgctc aataccggca
    19741 ggttcttcct gagtcaaatc aatatttttt ataaactcat tagcaaaaaa caccgataaa
    19801 tcgcttaatg tttttataaa ctctttagct tcctttaagg gcgagaaaat taaactcaac
    19861 aattcatctt ttctcaactg taccgaggat atatacgtca gatttttctc cactatctgt
    19921 tttctaaggc caacagcaac ttctgctgaa gtctgcataa ccaaaggatg catgagtact
    19981 tcaaaaacat ctttatggtt gaaaaagttt tgaccgccct cgccgggctt atttttaagg
    20041 tgaatacggg caaatacctc tataagtgaa aaaaaagcag agttggctgc cggatacccc
    20101 atggtaatat tgatatcagg aatgtctgaa ggaatcgcgc tcaacaatgc tggcaataac
    20161 tgttcgtctg taagcaaaat agctgtttct tcaggcacag catccttccc caagtatttt
    20221 ttgaccagat taccggcaag tttggcttgt cccacttcag aaggaacgcc aatgcattct
    20281 attttcttat cattagaaag ttgtggaaag ttaaaattaa tggcagggtt gaaatttctg
    20341 aggttctctg aaataaagaa gcccgattca tggccttcct ggagcatttc ggcataatag
    20401 tcccaataaa aaagtgtttt accatttgcg gcaaaatgct tataaacaat attttccgct
    20461 tcggtcaacg cattaaaccc aataaatgca gtatattcaa aatcattact ttgaaattta
    20521 ccagccataa cagactctgc cacatccctg tatatcatcc cttcataagc aaagccttgc
    20581 tgacgcagcc tttctttaaa caaggaataa atctcttcca aattaagcca taagtttgag
    20641 aagtcatgct ggtgcttgct tacattatcc ttctcgaaat gttgccagaa acgacggatg
    20701 gtttcaagct gttcctccgt caaagcctcg aaagtttcgt ttatattttt ataggcagca
    20761 aggttcctga acaaaagatg ggtatttacc agatatttgt caatgtcgtc aaagtctgac
    20821 agcatcacct cgccccaata gtagaaattg tcaaagcttt cacctgtttg acgaacatcg
    20881 gtataagctt tgtatagcgt aaaattgagc aacaggtcgt ttgctttgct caagttagta
    20941 tattggccaa aaaagtcgga caccgtaaaa taacccggtg cccacactgg tttctggact
    21001 atctccgata aatatttctt aaaaaacagg atagaacgcc tgttgggaaa aacaagcctt
    21061 accgtgttga gtttttcacc aaaacgcttg tataaatcgt ctgcaacaag gtaaagaaaa
    21121 ggcttcatat tacaatatgt tgaaattaat aaaattcaac atcaaaacta ttgcaacaac
    21181 aaatcttata gatgttttta cgtttctttt tttcgtcaaa aaaagaaacc aaaaaagact
    21241 ggctgtgaaa actcgctccg tgaagaagtg gcaaacagcc attatacaca aaaagtcaga
    21301 atatttcgct tcttcacttc gctcggacag ttcactgcca aataaggaat ctctttttgt
    21361 ctgcttcgca gccattgatt caaaacatct tcctttacaa cttttcatta tgaatttact
    21421 tgaatataaa agtagttagc tgctactagc gctgcggttt cagtacgtaa gcgactttca
    21481 cccagcgata ctggtttata tcctgcattt acagcctttc gaacttcgtc tggagtaaaa
    21541 tccccttcag gcccaattaa cacggtcata ttttctccta aagcaggttt tatggtaaat
    21601 aaactctctg tcttataatt ttccccaata gcatcagctt ctatacaatg agctatgtat
    21661 aaagagccag atttattggc agtaatgaac ttatcaaaag ctacagcctc atttattact
    21721 ggcttaaata agttcaacga ttgcttcata gcagaaaggg caattctttc gagcctttcg
    21781 atattgatat tgcgacgttc actgcgtgaa cagacaatcg gggtaaatgc agaaagcccc
    21841 aattcaacag ctttttcaag catccattca atgcggtctg catttttggt gggcgctaca
    21901 gcaagatgca actcttgcgc tttacgccgt tgccttttca gctcttcctc gatccaaata
    21961 atgcatttgc cggagttaat ctcagccatt gagcctttat acaaaacgcc gttgccatct
    22021 gtaacacaaa cctcatcgcc tttgcgcaaa cgcagcactt ttgaggcatg tttcgactct
    22081 tcttccgaaa gaaaacaatg ctttccgtct atctcgtttg aataaaaaac ctgcatgtat
    22141 tattcccttt tcttttccca aatttccaaa tcctttggct ggccatcctc aacagtgaac
    22201 ctcacggcag tttgcacatg atgaaaaccg ctaatgcccg ctgctcccgg attgatatgc
    22261 agcaacccca attgcttatc attgattact tttaaaatat gcgaatgacc gctaatgaaa
    22321 agccccggtt ttaatttcag caataattct ttgattccat attcgtatcg tccgggatac
    22381 ccgccaatgt gggtaataaa aacccccagc ccttcacaat caaaatgcaa ccattccttg
    22441 cactcatgac gcacttcaaa accatctata ttgccataaa ccgcccgaac tggcttgtac
    22501 atcttaatat aatcaagcgt ttcaacattg ccaatatcgc cggcatgcca tatttcgtca
    22561 caatcagcaa aaaaatttgc tatatccccg tcaaaatagc catgcgtatc tgatatcaaa
    22621 cctatgcgtt tcaatttttt tgtgggtaaa gatagtaatc aatgacgaat tacgaattac
    22681 gaatgcctaa tgacaaatgc ttaaaaatac ataaaatttc ctttgttcag tttgcaacaa
    22741 aaaaaaaggc ctgcattgct gcaagccttt atattattta tgttgacttt ttaaaaagta
    22801 tcataatgtt tgtcaacatc atcttcatgg cccatcttaa tatcagcttt tatagcagga
    22861 gatttgactt ctggtttatt ggttttatgc ggctgttgca ccggttttac gattttttct
    22921 tttttcacaa accttgaagc agcatgatct tcaacttcag ttttgtaaaa tgcaatcaac
    22981 tggcgcaact gttcggcctg gctattaagt tcttctgcac tggccgacat ttcttcggca
    23041 gcagcagcat tcttctgtgt tacggcattt aactgcatga tagcattgtt aatttgtgcc
    23101 gcacctgaat tttgttccat actggaggcc gaaatttcct gtaccagcaa agccgttttt
    23161 tggatatccg gcacaatctt ttgtaacaaa gtccccgact catcagcaat cttaacgctt
    23221 gacttggaca actcatcgat ttgttttgca gcattttggc tattttcggc cagttttctc
    23281 acttcggcag caaccacagc aaaaccttta ccttgttcgc ctgcacgagc agcttcgatg
    23341 gcggcattta tagccagcaa gtcagttttt tcggctattt cgccaatgat agaaatcttg
    23401 tctgcaattt gtttcatggc atctaccgta gtggttaccg ccttactgcc gtcgaggata
    23461 tcggtagaag cacggacagc aatattttcg gtttgtttag cattatcggc attctggtta
    23521 atgtttgaaa ccatttgttc catactactt gaaacttctt cagttgacgc agcctgttct
    23581 gtagagcctt gtgaaatctg catagcaaca gccataagct gttcgccagc ctgaacaata
    23641 ttttcgatag ctgttttgaa ctcgttaatc atagaagcgt tagattttac catatcattg
    23701 agggcttcca tcagttcgtc attttttgaa cgcatttcga gcgatatggt gaggttgcca
    23761 cttgctactt cttttgcctt atcaataatg gtattttgcg aaacaataag gtcgttaaga
    23821 ttggccatga gggctttaaa atcgccattg tactctttgg tgttcaattc aggcatgtcg
    23881 ccttttgaaa tccggtcaat atactcggca gcaatagtca gcggggctat tacagtatct
    23941 aatattttgt taataccttc tactattggg acaaattcga cactgaggtt tttagcgtgc
    24001 gaacgctttt tcaagtctcc atcaagagcg ctatcaataa gcatcttggt ttcattctcc
    24061 aacctttcaa tatttcttcg gataactaat gacatgacat atacaattac agaaagcact
    24121 gcaagcagga gtattccaaa cccaataaga taagcccttt gttctttggc agccttcaaa
    24181 aaatcatcat cgtaaacccc cagtgcaagt atccattgca atggcctgta atgtttaaaa
    24241 taagcagttt tagtccttgc tacaggctct cccggatttt tccaatcata ctttattttc
    24301 ccatttttaa ggcgacaaat atcctgcata aacaaatatc cattttcatc tgctgtatta
    24361 taaagatttt cgccttcgag ctttggatgc acaatttctg ttgcagtagt atcaattata
    24421 aagaaatagc taccttctcc cacaataatt ttactaagct tagctttcat gtcattgtaa
    24481 atattatctg acaagccaac ataaatagcc cctattactt ttttgttgtc atcaaaaaca
    24541 ggacgatacg aagacaacac ccatttgtta agcacttttg ccaggcctgt atattctctg
    24601 ccttgcgata ttgcctgata aacaggtgaa gtttcaggaa tataagtaaa aacagccctt
    24661 ttacctgttt catctttcaa tgttgtagat atacgtacca ttccttcagg aattacggca
    24721 aatacggttg aagaacaacc catcaaccct gaaatataat caacatgagc tgtattattg
    24781 acaagtccca ctccgttgaa catcaaattg ccaagcctga ccatcttggt ttcattgttt
    24841 tcctgatttt ttatctctac aaagttttga ttattggcgt tataagtaaa cttcccctta
    24901 aactcctgca ttgccaactt catacttgta ttgacaattt ccaaatgatt tttgtaagaa
    24961 atatccatat cttcaaaaac agcatcaaca agcagttgaa tatttttatc catcaggtct
    25021 gtggtcattt tggtagtgtc tcgtgaaagg aagataacaa acacggtaag ggtaaccacc
    25081 aacgacactg caatagacaa gaacaacctt gtgcccagcc taagtttgtt taacgatagt
    25141 ttcatagctt taaattttat aaattagtaa cttctttttc ttgtacattt tgaatatccg
    25201 gcgtttcggc atttgaaaac accttatcga cattgaggag caggatgaac gaatcgtcaa
    25261 ttttgaaaat cccttgcaga aattcggtat taaacttttc gctcatttgg ggcacaggtt
    25321 ttatggcatt atcatctata gttatcacat ctttcacctt gtcggcaatg gcgcctatcc
    25381 tgaaagaccc attttgaccg gacaagtcga ggacaatgat aacaaacgaa gacttagcat
    25441 cattttcggg aaagttaaac ttggaacgca aatcaaaaac tggtactatc tcgccccgaa
    25501 aattgataat gccttttaca taagcaggtg cattgggtac ttgtacaata ttctgttttt
    25561 gcagtacttc aagcaccttt actacatcta cggcaaaata ttggccgtaa accagaaaag
    25621 acaaatacgt acggttcata catcaaggtt tttatagttt ttcacttaca atatttatac
    25681 tttctgggct acattaatta atgaagctat atccagcata aaagcaatac ctccatcgcc
    25741 caactgactt gcagacgaaa cgtagttttg attttcaaag gtctggccta atggtttcag
    25801 tactgcctgg tgttcgccaa taatcttatc ggcaagcaag gctatatttt tcccgttttg
    25861 gttcaggaca ataagcttca cttttttatt gtaagcccct ccaaggttaa agctttcgcg
    25921 aaggtcaaca aagcgtatca gcgtgtcgtt atatgggatt gtcccggtat tacggcagct
    25981 atcaagttca acaagcgcca tctgctgaca aatcacaatt tcggaaatag gcacaatgaa
    26041 atatgacttt tctaccttga aaagcagagt atcgataatg gccagcgact gctgcaactt
    26101 gagcgtaaag gttgtaccct tgcccggtac cgaattgacg attatttcgc ccctgaggtc
    26161 gcggatgcgt tgttttacaa catccatccc cactccacgg cccgaaacct cagtcagact
    26221 ttgtgctgtt gaaaaacccg gcaaaaaaac caagtcgtat atttccttat cggagagctt
    26281 atcatttgcc ttaagaatgc ctttgtccac agctttctgc cgaacttttt ccaaatcaat
    26341 cccggtaccg tcatcttcaa tattgatata cacaaagttg cccgactgaa aggccgaaag
    26401 ttttatcatc ccggtttcgg gtttcccttt gcttttgcgg acttctggca actcaatgcc
    26461 atggtcgata caattgcgaa tcaggtgcat tagcggttct gtgaggctat caatggtatt
    26521 tttgtccaac tcggtttcag taccctgggt acggaactcg atttttttgt tcaactgttt
    26581 ggacaaatcc cgaataagcc tttgaaaacg cagcaccgtg tcattgagcg gaataagacg
    26641 gatatcaagt gcattgttcc tgaaaagctt tgacaggctg tcgatctttt caaactgcgc
    26701 acgcatagcg ctgtaacggt catcgtttag gttgacctgt atttgtgagt ttaccgtaat
    26761 aagctcgctg actaagtaca taagattatc gagcttgcgt gagtcaaccg atatgcgtac
    26821 aaaattttgc agctcctgct tttcttcttt tttaacaggc ttttcagcaa taatatcggg
    26881 ttctgtaacc tctttcttgg ggaattttgc atcctcaaca aaacgaagga ttgatttttc
    26941 gccttcgttc ataacattgt tagttttttt ttctttatcc aaaaggtttc ctttagaaag
    27001 gttttgaata atgcaatcat cctcgatgaa cataaacacc tcgcgaatgt catcttcgct
    27061 ggcactggaa ataaggaaaa tattccagta atcattctct tcatcactaa gggattctat
    27121 cctggccacc tgaaattttc caagagtagc aagctcttca aaaatattca taatattaat
    27181 ccccctgaaa tacatcattt cggtggtacg gacaataata ttccaggttg actcaatgcc
    27241 ttcgtcccca gctgggattg gctccgggtc agcatgccca gaggcagcat catttttatt
    27301 atccaggatg gaagatatct cggacaacag tttattatgg ttggcttgat tttgtttatc
    27361 ttccaacctt tcgtcagaca aaagtttgcg gatatggtca acagaagcga aagtaacatc
    27421 aaacaccgct ttgtcgaact ttagcttagc gtcacggata gcctgatata cgctttccat
    27481 gtggtgggtg tactcactga taaaatcaaa gccatacatg ccgctcaccc ctttgatggt
    27541 gtgcatggca cgaaaggctg actctatcag ttctttgtct tgcggatttg cttcaagctc
    27601 gagtaaatcc ttttcaagcc tgtcgagcaa gccaatggcc tcctcaataa acttatctct
    27661 gaatttgctt aaaataggat ccatgagtaa caggttttgg gcaaaattac cttccctgcc
    27721 accctcaaaa catcgtacag ctgttgaatt ttgtagttaa aacaatgatt tgcgtgtagt
    27781 acatttgtac tacaaaagtt tattatggca ctttcacaaa gtcattctta atggcataaa
    27841 atataaggct tgcggtgttg ttacaccctg ttttggccat cacccttgac ctgtgcgtgc
    27901 ggatagttgt ggtactaata ttaagttttt cggcaatttc ttcgtttgaa aggccagagg
    27961 ctatatgcct taacacttct acttccctgg ccgacaaatg ttgcatatta ttgacagcca
    28021 ctttagaatc tttgctcaat tgggccttaa aaccttccac aaggttcatc aacagttcgt
    28081 ttgagaaata acttttatta ttgtacacat ctacaatggc attgagcaac tcactaatac
    28141 cagaagactt caacacaaag ccttttacgc ctgcctggag cattttgaaa taatactttt
    28201 cttcaccaaa cattgtcaag gcaattattt tcaaatcggg atgcttttca tgggcttttt
    28261 ttgatgcctc aataccatcc ataacaggca tggcaatatc catcaataca atatctactt
    28321 tcaggctatc aagtttttcc agaaagtctt ccccatttac agcctcgcca accacttcgg
    28381 cctttgagct gccattgata agcaggttga ccccttcgcg gaacaaatcg tggtcatcaa
    28441 caacaaaaac cttgattttc tttgcatcca tagctaacaa ttaattaatg aagttaaaat
    28501 aaacccctga ttgggtttgg atatcatttt gtatttccca ttcaaatgcc tgatgcggtt
    28561 tataatatta taaagcccca ttccattgtt actttcgcgc actttagcaa tatcaaaacc
    28621 tttgccattg tccataaagc caagaaacaa ggaatgttca tattttttaa gttttatcag
    28681 aacagatgag catttagcat gtttcaacga attgtttaaa cactccagca caatcctgta
    28741 caaatcagtc tcgatagttt cattgacctg ttcttttaag ttggtatgaa aagaaatttc
    28801 aacattgggg tttagcccaa ttttattgat gtaagtagag atggcataag caagcccaaa
    28861 cttttgcagc acgtgagggc tgaggttatt ggctatttct tttacggtat tgaccgcctc
    28921 gtttactgtt tttattgaat gctgaatgat tttatcttta ttaacaggct gttcagggtt
    28981 attcatccat tcgaggtgca tcttcagggt agaaagaata gggcccaggc catcgtgcaa
    29041 ttcgcgggca tagcgttttt ggtcttcttc ctgcgtatga atgatagcat ccagaagctt
    29101 tttatctaca agtttttttt cagtgaaatc tttaaccaat gacacgataa tatctttatt
    29161 ctcagcatca gcataagagc tgctaatttc aaaatatttc actttggcac cgtcattaaa
    29221 ctctatttct ttgtatcgtt ccacttcacg tgaagctatt tctttgggca agtttaacaa
    29281 tacaggtatt ctgttttcta tttcacgcgt aagtaaacta aactggccgt taagagcctc
    29341 cattcccaac attttgaaaa aagccggatt tgcatcaatt atttgctttt catcattgat
    29401 aaaaaaaatc ccttcatcag cattctcaat tacttttctt attttccctt cactttgttt
    29461 taaggattga agcgtatcgt acaattcact attttttacc cggagctctt caaaaagata
    29521 ttgattttcg tgtttcaaat catattctct gatggcgctg tcaatagcca gtcttatttc
    29581 attggcattc cagggctttg taatatacct gtaaacactg gctttgttca aagccccaag
    29641 tataatctcg cggtcaacaa atgccgagaa cataattttg acaacatccg ggtataacgg
    29701 gtttatacgt tggataaagt caatacctga ttcgccgggc atataataat ctgatatcag
    29761 tacttttaca gggacagtcc ttaaaagctc ttcggcttct ttggtattct gggcaacata
    29821 gacattgtaa gtattttcga agtacaacct gaacacatcc aggctattga cctcatcgtc
    29881 cacgtacaat atactattgt tgctattttt cgacatatta tgtttaaaca attgtttaac
    29941 atataaatat acgaaattga aacaacattc gctattgcca gcaaggacaa aataacccct
    30001 tcgatggcta aaaatttact ataacgccag ggcctgactt ttactttgaa acgggcatga
    30061 ttagttaaac ccaaacaagt atgattcccg attagtcggg aggaggtttt cttcggactt
    30121 gcggtatatt gtctataagt gttgggatat ccaatacata ttttttagcg ccttttttga
    30181 aaaaagttga ttttttgctt cttaaacagc aatttaacct acatcaaaga caattaagca
    30241 aatttcaacc ttttccataa tcggctgcaa actccgaagg cgttttgcca aaatggtcgt
    30301 gaaaacagcg cgtaaagtac gaaggtgaag aaaaaccggt ttcataggca atttcagtat
    30361 agctcagacg tttttcaagc accagcttca cagctttttt caacctaact gtccttacaa
    30421 actcaactat tgaaaggcct gtaatagcct ttacttttct gtatgtctgt actttactta
    30481 gggcaacaca atcggccaac tggtcaacat ttaaatttgt atcggtaagg ttgtcttcaa
    30541 taaaagtcgt taactttttc aaaaactcat catcaagctt agaagttaca gccccttcgg
    30601 gaattagtgc agtttctttc agatatcgcg aatagatgga ctctttaaag ccaatgagcc
    30661 tcaacacttg tatcttcagg tggtcaacac taaagggctt taccatgtaa acatctgcac
    30721 ccatttccaa gccttcaatt tgttgattta tattagcttt ggccgaaagg atgataatag
    30781 ggatatgaga agtcccaagc tggtttttca atgttttaca caattcaatg ccagaaattt
    30841 taggcatcat cacgtcggta ataataaggt cgggcatcat ctcgttagct attctcaacc
    30901 cttcttcgcc atcagcagca acagaaatac taaaatctgt ctttaattca tcaacaatat
    30961 aatttcgtat ttcttcttca tcctcaacaa ttagcagatg gtaatgataa gtaggcttag
    31021 gttctaaatt ctttccttgt tcatgattga tagcaaaaga ggtatttttg tctttattta
    31081 ctgtaaaagc tgcttgttca aaaggcaaat aaaactcaaa tgtagtctca acattaggct
    31141 cgctttttac atccaccatg ccattgtgaa gttcaacata actttttaca agagaaagcc
    31201 ctattcctgt cccggctata tttcggtttt gcacattatt gacctgataa aacctgtcaa
    31261 aaacctttga aaggtgttct ttgacaattc cttctccagt attgacaacc gaaaccttta
    31321 caaaatcttt attaaaatta ttcacattct caatattaac taatattgta cccttagcag
    31381 gggtatattt gaaagcattt gacaaaaggt tgaaaatgac tttttcaatc atcatcttgt
    31441 ccataggtac ttcaatagcc tcttgagggt tattgacttt aaaatcaata ttcatcgact
    31501 cggccaggct gttgaataat tgacaaactt cttttgtcaa tgcaacaatg tcacacaaat
    31561 aaaactcagg cactaaaaga tttttctcaa ttttgctata atccatcaac tcgttagtca
    31621 gtttcaacaa gcggttgctg ctttttaaca cgatattgat tttttcttta tttttttcag
    31681 agagctcgtt actttttgcc aacttctcta acgggctaat aatcaaactt aaaggggtac
    31741 ggaactcgtg cgagatatta gaaaaaaact cggttttcat tttatccagt tcttcgagct
    31801 ttttcctttc gtagcgttct ttttctataa cactttgttg taaaactttt tgaatggtaa
    31861 gcctcctgta tatcaaaaat aaaatcaaga taacaaaaaa gtaaatcaca aaagctgttt
    31921 tggtttgcca gaaaggaggt aaaaacctta ccttcaacaa tttagaatta gttttttttg
    31981 ccattccggt ttcttgtact tcaaggaagt acaaacctgg cttcatactg gaaaatgaaa
    32041 tattcctttg gttagggcca agcataatcc attctttact tttctcaatt ttatacctgt
    32101 aaagaatttg cccggctgct ttaaaattta atgtagcaaa ttcaacactc agggtatggt
    32161 tgtatggtac atttatttca ttgcattcat tgatatcaca cctgatgata gtatgcccat
    32221 caactttctg cattggtaca acggtttggt tatcaatttt tatatcggta aaataaaacg
    32281 gaagattagc cccactacta tatgtcgaat cagtattgag cgaagccaac gccccgggag
    32341 tagaaaacca aacattgccc aaagcatcat aacacccggc atttaagata tactggttaa
    32401 aatcatcgtt gtgggctgac aaaaaatttt ggacagagaa gttttcgggg tttattttga
    32461 ttatgccaaa atttgtggcc gcccacaaag cccctttttt atcttcaaca atattgccaa
    32521 tgtcatcaga tgacaacccg tcacttttac ttaatacttt tagttttgcc caaggagttt
    32581 tcaggtaaca caaacctgct ccggtaccaa tccatatacc ctgtttgctg tctttgcaaa
    32641 tgctcctaac attcaactgg ggtaaatctt tgtttatata cgtcaatggt tcgacagaaa
    32701 acttaaaacc cggcaattct ttaacagaac atacccctcc gccagaggta cccacccaaa
    32761 tttcgttttt ttgtacttca cacattgtaa tggcaggaaa aatggggaaa tcaaattcat
    32821 ctttttgttc atcagagaaa atgctcttaa cagtgcagtt ttgtgccagt tcatccggag
    32881 ttgaagcgtt ttgaaaacct tctttcatcc gtaaaacata taaagtaccc caagagccaa
    32941 cccaaatata acctttagaa tcttcataaa acttggtaat atatgaattg gcacggctat
    33001 cagacgaaaa cagcaaattg gcatctaaag gcaaaacgcc ccctcctttt ccattaaaca
    33061 actgaagggc ttttttgcca ggaacaacgt aaatgccttt tcttccggac actatccaca
    33121 agttgccttt gctatcctga aagcaataag aagccccgtt actctgcaac ctaagacgag
    33181 agcctgcagt attattgaac tgtaaaaagc ttttttcttt agtgtcaaac aaatatatcc
    33241 catctccatt ggtggctacc caaagcttgt ttttatcaat tgactgctga acagataaaa
    33301 actcgtttac cagatcgctg cttttttttg tagaaaaata agagatgaaa ctttcctggc
    33361 tatacaattt agccagtccg ggctggttca tactcagcca caaaataccg gaattgtcga
    33421 tgtatgcctg gtttataata tttgacctga tgttttcttt gctgttttta tcgtttcggt
    33481 aatttatgta cgaaaaagaa gcattggcat tattaaaagc atacagcccg ctaccatagg
    33541 ttgccacaaa aaggttcccg ttattgtctg gcaaggctgc ttttactctt ttttctccaa
    33601 caatcttatt gacatagtca ataaatggag tacttgtttt ttttagcgtt aatgtatcaa
    33661 ctaaaaaaac gcctccatcc cagcttccgg caagaatttt attgtcaagc accgacaaac
    33721 acctgattga gttttgaggg aaagaaatga tttcagaaaa ctcatcaatc tcaacattat
    33781 acgcatacaa ctgagcattt gtacctaccc acaaaatttt gctccctgct ttgtgataca
    33841 aacaattgat gactttatcc tggcttgtcc tgttggccaa atcgggtatc aggcgtttat
    33901 agctgatcgg ggtaccatcc gacaggttta tatttttatt tatttctaaa agtccaatat
    33961 ttgaagctac ccaaaccttt cctttcagaa atagaatctg gtttactacc acctcttcgc
    34021 tcaattttag gtttaagtgc accagctcaa aagtcttgtt gagcaaaaca aaaatccctt
    34081 tacctttagt gcctatccaa atgttttgca ggttatcttc agcaactgaa tatacttcgc
    34141 aattcaaatc tgcattagta atagtactaa ggtttctaaa gctataccca tcgtacctga
    34201 aaaggccatt tcctgagcac agccacaaaa aaccttttga atctttaaaa acagcattta
    34261 ccgaagaggt tgttaaacca tctttatacg aataaatgtc tattttcaat actttttcac
    34321 tggcaaacaa actaatgttt acgaatagaa aaaaagcaag tgcagaatac agttttttct
    34381 tcatcctttt tataataaaa caatccaaag aaaaagcaag atacacatac acaaaaatag
    34441 caggactaaa tactcagcca gagtttcttt catatatttt ttcaacatcc aaaaatcagg
    34501 ttataacttt atgggattta atcaacagac acaatttttg tcccctttgt gatgccattt
    34561 tcattaaaag catcaatagt aaaataatat ttttcaaaac ggttcaggct tcgaataatc
    34621 aacgaatcgg agcccagaac ctggtaaaaa tgatacagtt tgtcttgctt actgccatat
    34681 ctgatggtgt aaccaacatc ttcggggttt atttgccatt ttagcttaac aatgcaacgg
    34741 tcagtatctg accgggtaat actgaactta tctaccgcct ttggcaaagc tcctgcagca
    34801 ttaccaaaca cccgcaaacc tgacaaagca aaggttccat ccgggacatg cctgttggta
    34861 agctttatat atcgagcctt aattggcttc ttgagctcga tataatcatg gggcaaatct
    34921 tttttgctcc ccttgcggct tttgattgtt tcccagtcct ttttgtcaag cgaggcctcc
    34981 aagtcgtact ggcaataaat atctgtattg cggccggtga tagtggtatt attctcggca
    35041 taattaagct gcacagcatt gacaatacaa actttctgaa ggtcaacgat tatccattcc
    35101 cctctttcac cagagcttgc actccaatag gtccgaacat cctcatcaac tgcattttct
    35161 ttaggataat caggcaattc agaagatacc attactggct taccataaga aagtaacatc
    35221 catttaggag acaaatcttc tggagaagaa acctttttgc ggggcaaaat cataggaaaa
    35281 tcgccataag ccgtataagt atagagctca ttgtctttgt caaaaaacat ggggaataag
    35341 cctatacgac gctcaaaaat atgcttacgc gaaatggtca tggtcgaaat atgccaataa
    35401 ttgccatacc tgtcctgaaa ggtagaacta tggccagccc cggcaataaa gccccccggt
    35461 ttgtacgaaa ttgggttatt aggggcaagt ttaaaattgc ccaaagggct atctgaaaca
    35521 taaaccccat cgcaataacc tttgtactga gttcccggag ctgcatattg cagataatat
    35581 ctgccattat gctttgtcat ccaggctcct tccatccagg ggttttcttt cagctcgtta
    35641 ttatcgccct ttctttccca tccataatca tctttatggc tatcaataag ctccttaggc
    35701 tttccaatag gattgaaatt tttcgtattg atttctatcc cataaattgg gcttttatct
    35761 gaacatccgt aatagaggta catccttttg ttgtcatcaa caaataagca agggtccatt
    35821 tgcccgtaag gaaaattatc cctaatctct tcccatttgc ccgacttagg atcggcagac
    35881 ctatatattg aagggctccc gaacgaggcc atgaaataca tggtatcatt cagcactacg
    35941 gcggtaggtg aacggtcttc tatgggcaaa tcttcggtag taataagttc ccactttgct
    36001 aaatcttttg agtaccagta ccctccagat cttgtcaaaa acaaaaaata ttcattttta
    36061 aacagcacta ctgttgggct ggcagcttca cgaaaagaag gcccgctaag ggtaaacctg
    36121 tagctaatgt ttatggggtt acaaattgta gtttggtcat attcatatag ctccaacttg
    36181 taataacaaa ggactgccaa taccagaaat actattagta aaagaaatac tttgacaaac
    36241 ttcatcgaaa aaaacttttt ccaaaataaa accaattgtc taaaatttcc ttatctcttt
    36301 gcattattct ttaagcaaat ggataatata tttctgacca gcctttgttt cgaaatcata
    36361 cagtaaagtt ggtttaagct caactgctga aagttttgcc acatccgaaa ttatagggct
    36421 ttttatttga ggcacagcaa aaaaaggatt ggggttaacg ccttcagcta ctttcaactt
    36481 taaacctcct gctaattgaa ccttatttgg cacacgtatg cgacaattgc caccaagatt
    36541 agattttata acaatttcct ttaccttgcc attttcccag ctaaagctga cctcaaaccc
    36601 tccataagca cgcaggcctt ttatttcgcc ggttttccaa tcatcgggca aagcaggcag
    36661 tatatgaatt gcgccatcat gagtttgcat cagcatttcg gttatgccag aagtacaacc
    36721 aaagttgcca tcaatttgaa atggcggatg ggcatcaaaa aggttaggat aagtacctcc
    36781 agatgcgggc cttacagggt caaccaatgt aagctggtct gttataagtt ttttggcgtg
    36841 gttaccatcg agcaaacgtg cccagaagtt gaccttccaa cccatagacc acccggtaga
    36901 tacatcacca cggtatttga gggtattgcg ggcagcatca aaaagttgtg gcgaagaata
    36961 aggcgaaatc tggttgcttg gaaacaagcc atataaatgc gatacatgac gatgctcatc
    37021 tttaggattg tcaaggtctt gcatccattc ctgcaattgc ccgtactgtc ctatttgcat
    37081 agggggtaaa cggtctagcg tcttttgtaa atcagcaatt aaagcattgt ctttgttcaa
    37141 cagcctggca gctttgatag ttttagagaa caagtcaaac aatatctggt tatccatagt
    37201 atttccggca ttgacagaag ccgaacgccc ggccggggcg ttttcgggcg agatagaagg
    37261 gcttacaaca agccacttgt gaacaggctc ttctatgaga aaatcctgat aaaactcgca
    37321 agctgacttt agtacaggat ataccgattc aagatacttc aaatcaccat tgtaaagata
    37381 cctttcccaa aggtgttgcg aaagccacgc cccgcccata ggccacatac cccagaaaga
    37441 gccgtcaaca acaccacata tgcgccaaat atcggtatta tgatgggcta cccaaccctt
    37501 gcaaccatac atggttttgg cagtattttg cccggactca gaaagctccc tgaccatctg
    37561 aattaaaggt tcatgcattt cagtcatgtt gcacttttcg gcaggccagt agttcatctc
    37621 ggtattaatg ttgatggtgt atttactgtc ccaggcagga tgtacctggt cgttccaaat
    37681 cccttgcaaa ttagcggctt gtccgccggg ttgcgatgac gaaataagca aatagcggcc
    37741 aaactgataa tacaaggcca caagctcagg gtcgttggtt tgagaaaaat ttctgacacg
    37801 ttcatcggtg ggtaaagccc gagctgcaga tgaacccagg tcaatgctta cacggttgaa
    37861 atactgctga taagcagcca catgtttttt atataattct gcaaacgttt tcttttcagc
    37921 ctttgacaaa tagttggcag atttttcggt ttcgttggct gtcaaggttt tataatcaac
    37981 aaaattggta gcaacagaaa tcaggatgag cgcttcattg gcattgctga cttttatacc
    38041 atcaggtaaa gcctcggctg caccacctgt aagctgcagt tttacccggg cattgaattt
    38101 cacctgcccg gttacgcctt cgtggtcgct ggagagccct gtcagttcga gggtagtatt
    38161 gtccaacacg gtcattgttt tttgcaaagg cccgttgata ccggctacaa aagacaattt
    38221 gccgggtttg tcggctgtca acctaaggac aataacctgg tcaggggcag aagcaaacac
    38281 ttcgcgtttg tagatcactc cgtccacttt gtaagatgca gtaaatacag ccttttcaat
    38341 atccagctcg cggtaataat cagtaaaatt ggtatgtcca aaagtaagca acaggttgcc
    38401 tacaggttga aacattgagc catgcaactg tttggcggtc aacttttgat taaccagatt
    38461 ttgggcttcg tcgtactttc cctcaaaaat cagctgcctg acaacaggca atgcttctaa
    38521 agcatcagga ttgtcattat tggaagggcc tccggaccag aacgttgctt cattgagctg
    38581 taccaactct ttttcgggtc cgccaaacac cattgcccca agacggccat ttccaagagg
    38641 cagcgcctcg ttccacacct ttgctggctt gttgtaccat agcttcagct ttccaccttg
    38701 ctgagctaaa gcattttgcg ctacaagtat gaagctaaaa aaaagtgcat gacataaaat
    38761 cttctttatc atcgttttat cctattagaa attattgcta attttcacta tttagtattt
    38821 atccttaaag tcgtttttgc gatgggaata ttcatccatt acaatttcaa atcctgcttc
    38881 gtcactaaat caaatgccat cagattaccc tgatgtaaat tttgagctac aactttttga
    38941 aaacgggttg gaactattga gaaaccttcc aggtttttga aacctggaag gtttaagctg
    39001 caaaaccaac aactataacc caagaccaaa tattaaatcc ctttcggatt ttcgacaggc
    39061 tctatttaaa aagcagttgg gcaaagttgt acaggttgtt acgccaaaca ggccaggtgt
    39121 ggccgccggg atactcgctg taggtatatt taatctttag ctcatcaaat ttcttaatca
    39181 ttacctgaca attattccag gctatatctt cttttcctcc ctgcgaaacc cagaattgct
    39241 taacattgct attaatagca tctgcattgg ctttcataaa atcatattgt ttgttggcca
    39301 aatcggcctg cataggcaca atccaacctg agctgaacac accaaggtaa gcgaaaaggc
    39361 tataattgtt gacccctgca tatagtgtct gaagcccgcc catagacaat ccggccaaag
    39421 cacgatgttg tgcatcggtt tcggcacggt agtttttttc tacaaaagga ataacgctta
    39481 acttcaattc agcttcaaaa gttttcagca cctcttcacc aaatgcttga gaattgaagt
    39541 ttccatccat catcacaaca agcataggtt ttgctttctt ttcggcaatc aggttgtcaa
    39601 gtatcaaatc tgtcttgcct tgtgtagccc aaccacgttc atcttcgcca ccaccgtgaa
    39661 gaagataaag cacaggatat ttctcgcttg tattttggtc atatccggca ggtttgtaga
    39721 tgtataagtt acgccagctc cttgtcacat ttgaaaaata gcgtttgata ctaatttcgc
    39781 catgaggtac atttttgata gcataatagc catcgccttt aaaaggcact tctataccac
    39841 tggccatgcg ccccattcca taaaatgttt cgctggcggg gtctgcaacg cttactccat
    39901 caataatcaa agtataataa tgaaaacctt cgctaagcga gtcggtagta acttcccaca
    39961 cgccatcttc accttttacc atatcatatt ttctaaacaa atcaacctgt accttttgtg
    40021 cttcaggtgc ttttatgcga aaaacggcac gtccatcggg caacacttgt ggatattttg
    40081 ccccacgtac attgtatgaa gaaggtgagc ccaatacact gtatttgtta aaattagcaa
    40141 cattaactgg tttgaaaagc aactgagaaa acatatacaa tgagtttttc caaaccttaa
    40201 aatcatgaac accgggttct atataataaa tatgaggcac atcattctta accatgtaat
    40261 cgtgggtacg ttggctaaaa gtaatcagtc cgtcgttgtc gccacaagat atccagagga
    40321 gtttcagttt ctttttagcg tcagccgggt taggcatcag ctgttcgggt tctctggtat
    40381 tgggagcaga agaaaaccct cccacccatg aaaatacatc gaggttgcca agcccaaaat
    40441 tcaaagactg cccgccaccc atagacaatc cggcaatagc acgatggccc ttgtctttaa
    40501 tcacagggta tttcttttcg ataaaaggga taaggtcgtt cagcaggtcc ttctcgaaag
    40561 tagcaaaagc ctggactttt tcaggttcga aaatattacc aacagcacgg tcatctttca
    40621 tagcacggcc attgggcaat accacaatca taggctcaag ttttttatca gcataaaggt
    40681 tgtcgaggat tacatgaggc tgcccttggg tgaaccattc cttttcatcg ccacctattc
    40741 catgcaaaag atacaacaca gggtattttt tacttttcga aaatcccgga ggggtgtaaa
    40801 tcaaagcttt acgggctacc ccgactgttt tcgactgata cgtaatagtg tcaatttttc
    40861 cgtgggctat attagtacgg acaacatcaa agccttgtgg ggcatgtttc tcagccactt
    40921 gggctataga aacaatacct aatgcaagta ttgcagaaag tgagagaata agttttttca
    40981 tgaatattag tatttagtgt atagcaatta ggagatagat ggttaatggt taatggttaa
    41041 tggttaatgg ttaattcgta atttttaatt ttt
//
LOCUS       DAOSPF010000029        40541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1028_length_40541_cov_7.007459, whole genome shotgun sequence.
ACCESSION   DAOSPF010000029 DAOSPF010000000
VERSION     DAOSPF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_40541_cov_7.007459"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..3247
                     /locus_tag="PK252_07495"
     CDS             317..3247
                     /locus_tag="PK252_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOT14590.1"
                     /translation="MNFNGLKLNFKGELQLDGTFKKIYATDASAYRQYPVMVAFPACN
                     EDISKLIQYATANKLTLVPRGAGTSLAGQVVAEANGVVVDISRHLNNITEVNVQEKWA
                     WVQPGVVLDELNLAMQAHGLFFGPETSTSNRCTIGGMMGNNACGAHSLLYGSTRDHLL
                     EMKGFLSDGSFVHVKPLNKQEFLEKMKLNTLEGAVYKHIFKLLSNTHTQKSIQEGYPH
                     PLLKRRNTGYAVDLLLDNEVFGHSQELFNFCKLLAGSEGTLFFATDLKINLLELPPKH
                     KGLLCAHFHSLDEAVEANLLALKHQPASIELIDDIIINCTKDSIGQKPNRFFIEGNPG
                     AILIIEFYAESEVVLQQMQRNLEADLRQNNLGFHFPFVTGADMSKVWAMRKAALGLLS
                     NIVGDAKPVSLVEDTAVFPELLKDYLHEFKAMLAKYHLTCVFHGHISTGELHLRPVIN
                     VKTPEGLQLFRKVASETADLVKKYRGSLSGEHGDGRLRGEFISKIIGKDNFDLCCQIK
                     QAWDPRGIFNAGKITATPPMDTNLRYVVNPQFDNIPTYFSFDDSAGYYRHIERCNGSA
                     DCRKGQLMGGTMCPTFMATGDELMSTRARANMLREAFQNFDGIWNTQKDTLEVLDNCL
                     SCKACKKECPSNVDMTKLKAEYLQHHYDAHGVPLKSRMIASLPVLFKLSSLSPAFFNF
                     ISQSFVGHRLLKPLLGFSNQRSIPVVYRFTLTNWIDKQKKAEAKNIKGKNKIWLWADE
                     FTNYLDVEVGMKAYKLLSLLGYDVHLLPMVASSRTYLSKGMVKKAKAIVNKNLAWFER
                     HYDGMPIVGIEPSAILTLRDEYLDLADACYKSIAQKVAQHSMPVEEFICNLYQARPEI
                     SSLFQDKEIHIALHGHCYQKVLSDTSMVKKMLEIPANSKVTELKTGCCGMAGAFGYEK
                     RHYQLSMQIGELSLFPSVRKLSSDTIVAASGTSCRQQILDGTGKKAFHPVEILYNLLT
                     NSHL"
     gene            4422..4868
                     /locus_tag="PK252_07500"
     CDS             4422..4868
                     /locus_tag="PK252_07500"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT14591.1"
                     /translation="MQERLVQLMQAKQLNAAKLADLLGIQRSGLSHILSGRNKPGYDF
                     MEKLLLKFPDLNAEWLILGKGNMLKLSVNPIQDIFSANNEVNNAANVVNSEEAPVYNT
                     IQKPSKPVNLDKVTDVNLLKNVENKQIERIVLFYSDKSFSEYSPEK"
     gene            4903..5925
                     /locus_tag="PK252_07505"
     CDS             4903..5925
                     /locus_tag="PK252_07505"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOT14592.1"
                     /translation="MYKTIIRPIFFLINPERVHHIVVTLVSSVRFIPGGKWLLKKVFA
                     IKTQPIKVVGIEFPNRVGLAAGFDKNASCFPYMALFGFGHIEVGTVTPKGQPGNEKPR
                     LFRLCKDNALINRMGFNNHGVDAAVEKLKKLRRDGVVIGGNIGKNTATPNEDAVGDYL
                     DCFIKLYDHVDYFVVNISCPNVTNLHHLQDKDSLSAILGAIVEHRAKQITFKPILLKI
                     SPDLNFNQIDDTLEVLKQYNIDGVVISNTTVTRDNLITDKSIVENIGRGGLSGKPIKE
                     RSTELIRYVAQKTNHTLPIIGVGGIMSAADAQEKIDAGASLVQIYTGFIYEGPFLVKR
                     INKQLQ"
     gene            complement(6256..6720)
                     /locus_tag="PK252_07510"
     CDS             complement(6256..6720)
                     /locus_tag="PK252_07510"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOT14593.1"
                     /translation="MTVVEIIQSMMAPAVMISACGLLILGMNNKYSMVINRIRALDEE
                     KRKLKQMAEENSLNAHQEKRFNSIDRQTGKLAFRIKMVKNAVVCYSTAVALFIITCLG
                     IGLNIVFNNFDISGLVVVFFLLGMLSVFLGIIYAVKEVMKGYEIIYIEIEES"
     gene            6908..7840
                     /locus_tag="PK252_07515"
     CDS             6908..7840
                     /locus_tag="PK252_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOT14594.1"
                     /translation="MVSFNEKNTTALNHVVKELSDPKSYELVYHKPQLNEEMISVEAI
                     REIVELAKEIIFPGYFGNNAVRADSIDFYMGVSVDKLFKLLVGQIKRGYCFSCNEDNC
                     SDCNNCVVDAESIALSFIESLPAIRQKMAKDVHAMFLYDPASKGYGEIIFAYPAIIAI
                     THYRIAHRLIELNVPIIPRIISEMAHSATGIDIHPGAVIGENFVIDHGTGVVIGETCI
                     IGNNVRLYQGVTLGARSFQLDEHGNPVKGVARHPIVEDDVIVYSGATILGRVTIGKGS
                     IIGGNVWLTQSIPANSRILQGKPRETLFDMGSGI"
     gene            complement(7837..8289)
                     /locus_tag="PK252_07520"
     CDS             complement(7837..8289)
                     /locus_tag="PK252_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT14595.1"
                     /translation="MKTENVKETVKKIFTDYLEQKGHRKTPERYAILDEIYSRSGHFD
                     VESLYISMKNKNYRVSRATLYNTIELLLETNLVVKHQFGKNIAQFERAFQCLQHDHLI
                     DINSGLVIEFCDPRIQQIIKTACEMNNFIPSHHSLYIYGYKNEESGKV"
     gene            complement(8383..9576)
                     /locus_tag="PK252_07525"
     CDS             complement(8383..9576)
                     /locus_tag="PK252_07525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT14596.1"
                     /translation="MEKVSNRVKALAESSTIAMNTKTKELQAQGIDVINLTVGEPDFF
                     TPKHIKDAAKKAIDDNFSFYTNVAGYPDLLEAISKKLKRENNLDYAPNQIIVSNGAKH
                     SLANAILTLINPGDEVIIPAPYWVSYAELVKLAEGTNVIINTEVESDFKITPAQLEAA
                     ITGKTKALLLCSPSNPTGSVYSKAELKALVDVLVKHPDIFVITDEIYEHINFIGGHES
                     IAQFAEIKDRVVIINGVSKSYAMTGYRIGFMAAAPWIAKACNKLQGQMTTNASTIAQK
                     AAAAALNGDQAVVAEFRQAFHKRRDLVYNAIMQIKGVKCNKPDGAFYVFPDISAFFGK
                     SAEGRTIKNSDDMSFYLLEKAFVATVPGSGFGNDKCIRISFATSDEKLVEALKRLKTA
                     LENLK"
     gene            9651..10571
                     /locus_tag="PK252_07530"
     CDS             9651..10571
                     /locus_tag="PK252_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT14597.1"
                     /translation="MGKVYCIGETVCDIIFKNGNPVAAKAGGSMLNSSVSMGRTGLPV
                     SFISEYANDQLGQMIDDFLKDSGIDTSNIFRYDNGKTSVALAFLNEKNNASYSFYKHL
                     PQERLAIQMPDFTEDDVLLFGSFYAINKEIRPTVVELVTKAKKAGAVIIYDPNFRKAH
                     LHELSDLMPYIIENMQMASIIRGSNEDFEMIFGSKTIADAYNEVSKYCKNLLYTENEK
                     GFYIQTPSVGTFIPVDAIVPVSTIGAGDNFNAGFIYGIYKAKVKNAEIERFTSSQWNE
                     ICHYAPIFAKQVCKSYDNYLSKEFAASYKL"
     gene            complement(10575..10880)
                     /locus_tag="PK252_07535"
     CDS             complement(10575..10880)
                     /locus_tag="PK252_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14598.1"
                     /translation="MKNIGKLRIINEKTENKLMNKKYHIPVIGHFSNKSIEFKSIIEA
                     SAATGIHYSLIFEACIGKIFKAKNVIWEFKKGNHYIKYKAFYINAATSYKRSVGFNG"
     gene            complement(11342..13387)
                     /locus_tag="PK252_07540"
     CDS             complement(11342..13387)
                     /locus_tag="PK252_07540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT14599.1"
                     /translation="MKKILYSLTFALCFTNFQTKSMDNALLFKENDLIQFDKFTALQV
                     ESATQLVMEDVKKYIAEIAAVDNSKRTFENTMVRYDDMQQRLSSLTNIIYLMAYTHPD
                     DSLREVCQKNIEVLSKFNNEVNLNEELYKAVKAYSTTNEAKKLTGYKKKALEDMLIFY
                     HLQGFDLSADKRKELKVILDSISTLSLQFSANIAACKDYLEVTEDEIKGLPEDYKKAR
                     LQENGKYKIELNHPSVVPFMELSESDDARKRLSILYSNRAADKNLDILQAVLKERKKC
                     ARLLGYASAADYIIADNMAKNAATVWDFENSLYNKIKPKALKDYQQLLDRKKLDNPEA
                     TKINPWETAYLRNKVLKEQYNVDRQLVKQYFEVNNVIQGILTICKTLYQVSFEEVQGA
                     SVWAPGVKLFEMKEGTKVVGRIYLDLYPRPDKYSHFACFGMINGKKTTNGYQIPTAAL
                     VCNFPQATADMPGFMSHDDVETFFHEFGHMMHFVMSRSDLSFQAGISNTHEFVEVPSQ
                     MFENWAWNYQSLCLFAKHYKTGEVLPKALYDKMLSARNLCSGMDALRQLQYGMLDLTY
                     HDKYDPDGTQTTTDAMIELNNKVSLFEYVPGTHMQASFDHLMGYGAGYYGYMWSKVYA
                     EDLFSVFEKEGVLNPSTGLKYKNEVISKGSTVNEMDIVKKFLGREPNQDAFFKSLGL"
     gene            complement(13604..14500)
                     /locus_tag="PK252_07545"
     CDS             complement(13604..14500)
                     /locus_tag="PK252_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOT14600.1"
                     /translation="MITALDQLLAVCNYPQKAAIVWPTDAGIFKAVKPFIEAKQIIPV
                     FCGNKPEIENLCHAYGLKPLQYTVIHSNDQVIAAHDAVVMAENHEVGILMKGMVSTSI
                     FVKAILHSTLLENILTHITIFKHPLQPKLVALSDAAINIAPDIETKVAIINNAVDLMK
                     KLGYGHPKVAMVCPVEKPNPKIASTQDALILKDMNLKGEIKNCMVDGPFALDNALSAA
                     ASEMKNIKSEVAGNADVLVVHNLDAGNILYKSIGVIAGQPVAAVVAGAKVPIVLTSRA
                     DNEITRYYSLLLALHLTSANKA"
     gene            complement(14579..15664)
                     /locus_tag="PK252_07550"
     CDS             complement(14579..15664)
                     /locus_tag="PK252_07550"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Catalyzes the formylation of AICAR with
                     10-formyl-tetrahydrofolate to yield FAICAR and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOT14601.1"
                     /translation="MKLKYGMNPYQDYAEVIDSKNALQLKNGSPSVINVLDALNSWQL
                     VKEASKALNKEVSTSFKHVTPSGVAVSKPLDEKEKQAYLINQDLSALASSYARSRGTD
                     RLASFGDFIAVSHVVDKQTALLIKSEVSDGIVAPGYTQEALEILCGKKNGGYVIFEID
                     AAYEPQEIESRDVFGITVRQKRNNLVISDALLSNVVTSNAAISPQIKEDLKLGLITLK
                     YTQSNSICVVNNGHVIGIGSGQQSRILCSGLALSKANVWYQKMLLDYSFLKNYTGLKR
                     TDIDQIIEQKRQETFADKIILNQLPDSCLLSDGFFPQKDNIELAHKYGIKFIATPMGS
                     VRDNEIIETANKLGITIINTGVRLFHH"
     gene            complement(15843..17072)
                     /locus_tag="PK252_07555"
     CDS             complement(15843..17072)
                     /locus_tag="PK252_07555"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT14602.1"
                     /translation="MKILVLNCGSSSIKFQLFDIEKQLVMAKGGVEKLGLHGSFLKYE
                     PHNGESVKLEGEILDHQAGIEYIMGILISPKRGVIKTEEEINAVGHRVVLGGEEFKGS
                     VYIDNYVMQKLIEYSALAPLHNPANIKGIQAMQNILPNVPQVATFDTAFHQSMPEKAY
                     LYAIPYSLYKKHGIRRYGFHGTSHLYVSKRACEILGKDYNNSRIITCHLGNGASITAI
                     KDGKSVDTSMGLTPVEGLIMGTRSGDVDPGVINFVMQKEAVDAFTVNTILNKHSGILG
                     ISGVSSDMREVRAAADQNNERAKLALDMYHYRIRKYIGAYTAALEGLDILVFTGGVGE
                     NSDLTRKEVCKGFEYLGLEIDETLNAAARGKEQVINKPDSKIKVIVVPTNEELVIALD
                     TKDIVNQRNAVSKSKLW"
     gene            complement(17119..18087)
                     /locus_tag="PK252_07560"
     CDS             complement(17119..18087)
                     /locus_tag="PK252_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOT14603.1"
                     /translation="MAEVLEDYAYKRTTQKKGNIQKIGVIGCGQMGQEICRIVSQFGM
                     DVVFLDISPERVSEILNSIRKQLDDQVNQWGLTETEKKGILSRIKGTTDYNDFSDCDI
                     IIESINSKKPYTNLESRKEIFRKVESVISPEAIITSNNSTLMITELASELKKPERAIG
                     LHFMSPATTIKIVEVVKGIDTDENTYNRVCTFAKMIDRQVITINESPGHVSTRLIVSL
                     INEACELLMEGVSSVEAIDKIMKEGYGMQLGPFELADRIGLDKVVKWMDNLFQEYSLY
                     KFKASPVIKRLVRANYLGRKTGKGFYKYENGKIVSGNVMTRELFNR"
     gene            complement(18115..19116)
                     /gene="pta"
                     /locus_tag="PK252_07565"
     CDS             complement(18115..19116)
                     /gene="pta"
                     /locus_tag="PK252_07565"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT14604.1"
                     /translation="MDLLQQIKANAKKHQKRIVLPEGLEERTLKAADEVLNEELAQII
                     LIGNRDKIFDLAKQFGLKSIEKATIVDPLKHEKKDHYIDMMVELRKSKGLTREEAAKL
                     IENPLYLATLMIKNGDADGEVAGAMNATGDVLRPAFQYVKTLPGISVVSGAFIIILKD
                     KEFGEDGMFVFADCAVHPNPTERELAEIAVATAKTTRAIAGFEPRVAMLSFSTKGSAK
                     HEMCDKVINATRIAKEMDPSLKIDGELQADAAIIPSIGKSKAPGSEIAGKANVLVFPT
                     LETGNIAYKLVQRLAHAEAIGPVLQGMAAPINDLSRGCSVSDIVNLIAITANQAAGK"
     gene            complement(19248..20090)
                     /locus_tag="PK252_07570"
     CDS             complement(19248..20090)
                     /locus_tag="PK252_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOT14605.1"
                     /translation="MLKNKITISGHLGSGKTVLAKKLQAYLSYEILSVGGIQRKLAMQ
                     YGMDTTTFNKYMETHPEIDIECDNMVSRYGKSDEPLILDSRLAWHFVPHAFKIHLIVS
                     KTIAGKRIFGDTVRENEKHASIEDTIRSNYERRKSEVMRFKKQYGVDIDDIYNYDLVL
                     DTSYVTPDEVFECVKNQYLLWQNGQKHQSLWLSPKNLFPTRLDDNELHTGADIVVSKA
                     NAWFFIQHNHKKVSEGLKNNLGIVPCQIDKTVNSNILANYNCEIAVKWANNHGFVFDE
                     FPSV"
     gene            complement(20130..23123)
                     /locus_tag="PK252_07575"
     CDS             complement(20130..23123)
                     /locus_tag="PK252_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT14606.1"
                     /translation="MRGKSIVFVLLCVSVFVIAKQSDKEIKTYPSVIDNTSDKWVDSV
                     FKSLTLEERIAQMIMPAVYPKDTQNISQQLINIAKYNVGGYIIFQGGVTTVARLVNKL
                     QDAARTPLILGMDGEWGLTMRFDSAQLYPKQMMLGAANDTALIYQMACDIAQQLRRLG
                     LHTNFAPVADINNNPRNPVINSRSFGESRENVTRLAMAYMKGLQDNRILAVAKHFPGH
                     GDTGTDSHLDLPFINKPAKVIDSLELYPFKQLIKNGLSSVMTGHINMPSLDTTGKPAS
                     LSPIIVTDILKKQLGFGGLIFTDALNMKGAQKKKEKEQIISAVIAGNDILLMPANIKD
                     AIDTIKRAVKAGVVPEDEINSRCKKILKAKVWLGLDKSRQKPSVNNVVADLQQPLYRL
                     LSRYEIEKAITIIHDPKNLLPVKHLDTCYIASISWGCDTSCVFQQTLGLYAPIDEYYV
                     NENTSDSVLVQMYSRLRKYNLVIGSIVNTDMRLSKNYGVTDKAINFFSFLADSCQLIM
                     DIFGSPYLMNRFPENVNFQSILISYDDKKETQALSAQLIFGAIKAQGKLPVSINEKWK
                     LGTGITWDNTIRVKYTIPEEVGISSKRLAPIDSIMNDAIRQKAIPGGVVYIAKDNKVF
                     YHKAFGYYTYDSTLAVSTNTVYDLASITKIAATTPLLMHYYEAGKLKLDESLGHYLKH
                     FKKKGFKDLLVQDLMTHRSGFKSFSTIYLNGLNCIDKSRLTKGNSHAVNCSTKLNKVY
                     YANYKDKYYAVEVAKGIYTFKNWNDTIEKEIINTPLAKNNPYNYSDLNFIMLGRVVEN
                     ISKSKLDVLSSKLFYNKLGAGTLGYLPLKKMDTSKIAPAENDTIFRKQLIQGYVHDPI
                     AAVYGGVCGHAGVFGTANDLGKLMHVFLNKGIYGGDRYFEDSTMNKFNSRLFAGNRRG
                     LGFDKPEPDTAKQSPVSRYCSDQSFGHTGFTGVMTWVDPQYNLVYIFVSNRVYPSAAK
                     NKLAEMNVRTKIQDIVYKEMIGQKK"
     gene            23271..23675
                     /locus_tag="PK252_07580"
     CDS             23271..23675
                     /locus_tag="PK252_07580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT14607.1"
                     /translation="MLSSEVKIRVRYGETDRMGYLYYGNYPLYYEVGRTELLRKHGFA
                     YKILEDSGILLPVADMKVEYYFPALYDEELTVKTSILKKPGVKIEFLYEIFNSENLLI
                     NKATTTLVFVKASDRKPCKPPKDFSDLVDRFF"
     gene            complement(24203..25405)
                     /locus_tag="PK252_07585"
     CDS             complement(24203..25405)
                     /locus_tag="PK252_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT14608.1"
                     /translation="MSILYFFQMSPVLSFLIFAGIMLIIDLLIFLLLKKAFYHHKYWK
                     TIFIIHISVAALFLFFLFYFYFIYVSYLNAEKTANMFEFTAQLIPLYIFKLLFVLLSL
                     GSLLIKKYRQIIICISFSVAFVLFLYLQYGYWFGRFNYTLYEQSLSTTVPNQFKGLKI
                     VQISDLHLGTVVHHDKKLAKMIALVNEQQPDLVFLTGDLVNFFAQEAYPFIGHFSKIK
                     ARYGVFAINGNHDYGDYYRWHSEKDKELNLRLIDSFYLKTHIKLLSNANVFIEKNNEK
                     IWVAGIENIGIPPFRSKGDVAKAMEGIPDSAFVLFLSHDPSIWDSSLKTIPRLAVTFS
                     GHTHGFQFGKRNGKKPWSPLMWSNIHSWCGLYQYENRYLYVNVGQSGSFFPARLGMWP
                     EITVFTLN"
     gene            complement(25499..25810)
                     /locus_tag="PK252_07590"
     CDS             complement(25499..25810)
                     /locus_tag="PK252_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14609.1"
                     /translation="MGKVNKVISLQNLTPELLELLNKKYPDGYNNHVIKVTTPKNDFF
                     YGVTLDTPEVSYLVKVPVKIDTNPDEIDEKDYDEPESSVDIDDNISDSEDFEADADDD
                     E"
     gene            complement(25983..27125)
                     /locus_tag="PK252_07595"
     CDS             complement(25983..27125)
                     /locus_tag="PK252_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14610.1"
                     /translation="MKKVLALLMTAAMFISLSAQKKVVNNAESLMEKNELAKAYETIQ
                     PALTNDETKDLPNTWFVKAQILQKISISPDSSLRKSFENAPKEAFETYKKVNEIDVKK
                     KFDKKINMQMEELARGAAVYGSDAYGVQNYSKSLEYFELVLEIYKNPVFNNFIDTSMI
                     FNCGIAANNAKNYDKAIEYFKKAADYKYNGGITYSLLKSAYLSKGDSVQALAVLKQGF
                     ELFPNDLNLIVDLVNYYLVGGQAQEALNYLNMAKQKDPNNTSFYFAEGTLYEKINDID
                     KAVAAYTKATEIDPKNFNAYYNLGVVYYNNAVKIFDKAANEKDDAKYNELTIQGNEEL
                     KKSIPWLEKAHAIDPKEEICAKTLKGLYFRMQMTDKLNAINKEMGW"
     gene            complement(27192..29708)
                     /gene="gyrA"
                     /locus_tag="PK252_07600"
     CDS             complement(27192..29708)
                     /gene="gyrA"
                     /locus_tag="PK252_07600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT14611.1"
                     /translation="MSEDQRIIKINIEEEMKTAYIDYSMSVIVARALPDVRDGLKPVH
                     RRVLYGMSELGVTSDSAYKKSARIVGEVLGKYHPHGDLSVYEAMVRMAQPWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLMKIAEEMMADIEKNTVDFRLNFDDTLEEPTVMPN
                     RIPNLLINGASGIAVGMATNMPPHNLGEVIDATIAYIDNNGITTEELMQYIKGPDFPT
                     GGIIYGTSGIKEAYETGRGRVLVRSRTEIETTETGREKIIVTEIPYMVNKLELIQKIA
                     DLINEKKIEGISYLNDESDREGMRIVMICKKEASANVILNKLFKYTQLQNSFNTNNIA
                     LVNGRPKMLSLKDLIHYFVKHRHEIVIRRAKFDLEEAEKKKHILEGLLIALDHLDEVI
                     KLIRESKTPEEARNGLMENFNLSELQARAILDMRLQSLTGLQRQKIKDDYDALVKLIE
                     YLNSVLSDESLRMKIIKDELMEVREKYADERRTEIRLDETEFNPEDFYANDDMIITIS
                     HLGYIKRTPLSEFKTQSRGGIGSKASNTRDEDFLEHMFNATMHNTILFFTEKGRCFWL
                     KVYDIPEGSRTSKGRAIQNILSIDPDDKIRAFINVKSLEDQDFIKSNNVILCTKKGII
                     KKTSLEAYSRPRQTGINAITIREGDCLIEAKLTNGQNELLLAVKSGKAIRFNEENVRP
                     VGRNASGVKGVTLADENDEVVGMVCVDSPQLTIMVVSEKGFGKRSDISDYRVTNRGGK
                     GVKTINITEKTGKLIAIKGVTDDEDLMIITKNGLTIRLSIAEFRVTGRATQGVKLINI
                     KGSDSIAAVAQVPKSQDEETPVVEGGVEESNVGEENLPAE"
     gene            29879..32413
                     /locus_tag="PK252_07605"
     CDS             29879..32413
                     /locus_tag="PK252_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT14612.1"
                     /translation="MDANFSQKIKEVLSYSKAEAVRLHNNYIGPEHFFLGILKDGEGL
                     AVEVLAEQLDLEDIKNKLELQLVSNSELNTIETEDIPMLKSAERVLKLVYLEAKHLKQ
                     ETVNTGHLLLAILRQEDNFVTDLLKEKNVTYQKIKEELSKRQPRASSDYGDEDDDRSG
                     PFGQQGKGSPGNTPQQSKSNSDTPVLDNFGIDLTKAAEESRLDPIVGREREIERLAQI
                     LSRRKKNNPILIGEPGVGKSAIAEGLALRIVQRKVSRVLFGKRVIALDLASIVAGTKY
                     RGQFEERMKAILNELQKTPNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEIQC
                     IGATTLDEYRQHIEKDGALERRFQKVMVEPTSPEETLEILNNIKERYEEHHNVIYTAD
                     ALEACVKLTSRYITDRHLPDKAIDALDEAGSRVHISNINVPERIINLEKEIEETKNEK
                     IKSVKNQNFERAASFRDKEKELLEKLEIEKDRWEKELAQNRVIVDSEKVAEVVAMMTG
                     VPVQRIAQAEGTRLLKMSEELKGSVIGQDEAIEKVVRSIQRNRAGLKDPNKPIGTFIF
                     LGPTGVGKTQLAKVLAKYLFESYDNLIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQ
                     LTEKVRRKPYSVVLLDEIEKAHPDVFHLLLQVLDEGILTDSLGRRVDFKNTIVIMTSN
                     IGTRQLKDFGQGVGFSTSAKIAQANDYAKSVIQNALKKSFAPEFLNRIDDVILFNTLS
                     KEHISKIIDIELKGLYDRVISMGYRIELTSEAKDFVIERGYDVQFGARPLKRAIQKYL
                     EDPMAEAIIKASIHEGDTLTIDFDSENQEINIKIKPLEIKDNVAEN"
     gene            32893..34272
                     /locus_tag="PK252_07610"
     CDS             32893..34272
                     /locus_tag="PK252_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT14613.1"
                     /translation="MTNKFSILLIVLVALGCSKKNTLEEKSLQANTNSISNNLQKQVR
                     IMGHWYGEGKKELLVREMVREFSLLNQDLSIELLFPQQFLKYKEESELYFVECDTIEQ
                     MVRKNSWPFEVLFCDQERYKKIGQQLNDPNWGEKYLVDFSNEPWFKHAHKDGLIETMN
                     LKARYGNIMPGIVLEGITNILFVSEYVENKLGIKVKDLDMTFDDFIAYAQAVDNYNKT
                     HSDKISFLSQQYKTVNDILFTQLATSIYEGDKYESREKGIAALKKAYAEFEKLSKYNP
                     TKQYMDYSGYTYDEAQRILYDKQCLFTMNPTWMILLWLKSNPEGTKQLRPCEIPSFAG
                     KRSPFYSGFYQVIFVVAKNSKNPEAGKRFMKYMASTEIADKWVKYSSCPTGMRTSISY
                     TDFGQNKYEKFFQHIQKKYGNNQAEVSLPKLFLNNNTTASIDYGLNELLSGKIKADEA
                     YQRTMRQIK"
     gene            34301..35761
                     /locus_tag="PK252_07615"
     CDS             34301..35761
                     /locus_tag="PK252_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14614.1"
                     /translation="MNSSINNKVREKLSLKIGLLIIATVILVLLSSGAFYISKFSSDT
                     NKRFYKQLAAPALLMSSGKLKYDAAMDLKTISSLVGDSVIYSLVIGVNQKIYYSNDST
                     ILDKKVDEVKFLSPYDVFKAPIKEPYYAEANNGNEVICISPLYFEDGKYLGYLFIATD
                     TAAMNKSKNSLILIFLFGSLITVAILSILIMILFHRYITIRIKTILHSLTIIKNGDLS
                     KKIELNTQDELGQIAQSVNELTLQFKTIIQEIIGEIERLRSSSVELNSSAQSMSEDAN
                     NLASIAEQVASSMEEMVSNIHLNSSNAMTTENISKQAAKEMDVVGAYSQESLKFIKEI
                     AQKISIINDIAFQTNLLALNAAVEAARAGEAGKGFSVVAAEVKKLAERSRAAADEIHK
                     LSSVCVAQTEKSVSSVKMLEPEIMKTVQLVQEISSATNEQNSGADQVNNALQQLNVIT
                     QKNSSNSENIEDQASALSQQANKLNEIVSYFQIEEK"
     gene            complement(35763..36992)
                     /gene="larC"
                     /locus_tag="PK252_07620"
     CDS             complement(35763..36992)
                     /gene="larC"
                     /locus_tag="PK252_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOT14615.1"
                     /translation="MVLYYDCFAGISGDMNLAAMIDLGVDADVLISELKKLKLSGYQI
                     KVSKSMRNGISGTLVDVLLESSPKPAFQLNIPLSATKGASQHKHKQVHRSYKDIRKLI
                     EDSSLNDNIKKISLGIFHRVAVAEAKVHNCEVDNVHFHEVGAVDSIVDIVGAAICVDI
                     LKPSKILASAAIQLGSGMVKCEHGVFPVPAPATIEILKGLPVKTGGMPFESTTPTGAA
                     ILAELVEEFTDLTNFSISKIAYGLGHKEDAKPNVLRVIAAETEAKDFPTEQAMMLECN
                     IDDMSSEVFDFVFEKLYAAGAQEVFVTPVNMKKNRPAFVLSVLSPVSLENTLINIVLE
                     NTTTLGVRTYPVTKHILQREILTFSTSLGKVRVKKSFWNNIVKFKPEYEDCKLIAIQK
                     KLPLLEVIRIIQTELKE"
     gene            37175..38101
                     /locus_tag="PK252_07625"
     CDS             37175..38101
                     /locus_tag="PK252_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT14616.1"
                     /translation="MKRREFIGKSLGATLLAGAGLSLGTTPDLFASESSSSKAGSYDL
                     VAVRGGEPEVMLDKALEALGGMQKFVKKGQKVVVKPNIGWDKSPEFAATTNPALVKRI
                     IEHVYKAGAKEVIVFDFTCDNPKKCYENSGIKKAVEEAGGVLVSGNNEADYKPVSVPK
                     GIKLKEAKEHKQILDADVVINVPVLKSHQGSTVTISMKNLMGIIWDRGYWHRNDLHQC
                     IADFATYRKPTLNIVDAYRVMMKNGPKGVSVSDVKLMKSLIVSTDIVAADAAATKLFG
                     LEPRDIDHIRIAHEMGVGNMNLDKLNIARLTV"
     gene            38115..39668
                     /locus_tag="PK252_07630"
     CDS             38115..39668
                     /locus_tag="PK252_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT14617.1"
                     /translation="MQYLRTIRIVTSVIIFAVAFISFVGLPALLLPLFIQGPVLKMQL
                     VPSILNFSVVSMASFAFIIIILLTLLFGRFYCSSICPMGTLQDIMNRLSKPFRKRKIF
                     RFTLPYKKTRYIILALTIVPLFLSSSITLALLDPYSNFGKIISAFFRPITYGLNNLIS
                     NILAHFDIYSVDPVIMGSISVTAIIYASVLLISLVLLVFFHGRLYCNSVCPVGTTLGL
                     LSKFSFFQLKIENTKCTQCGKCSANCKAECIDIKHKELDFERCIGCYNCINVCTEKAI
                     SYSKLSPVKVEKETSTDEAKRSAFKTLAATGLGLVFANKYAFSQHETGKKNTIEVKKK
                     HTVSPPGSESHKHFLAHCTACHLCVSQCPSSVLKPSFLQYGLEGMMMPYLDFEKSFCN
                     FECTQCSQVCPTGAIKPIDSKKKVTVQMGIVQFIKENCIVYLEETACGACSEHCPTKA
                     VFMVPYKGMITIPETNKDICIGCGACEFACPAKPNKAIYIEGNTLHLTAKKPKEEKQE
                     ESNMEEFPF"
     gene            39684..40433
                     /gene="larB"
                     /locus_tag="PK252_07635"
     CDS             39684..40433
                     /gene="larB"
                     /locus_tag="PK252_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOT14618.1"
                     /translation="MNKEYIDKILSQLKNGEIEVSQASELLKTLPYAELGYAKVDHHR
                     TLRRGYPEVIFGAGKTPEHIKGIVANMLNTTDSNILVTRCSKEASEQVLSICPTAEYH
                     PLSKALVIKQKEITKTESYIAVVTAGTSDIPIAEEAQITAEIFGNKVETIFDVGVAGI
                     HRLYDQVDKLRGAKVLIVIAGMEGALPSVVGGLVDKPIIAVPTSVGYGAAFGGIAALL
                     GMLTSCTSGISVVNIDNGFGAAYQASLINQL"
BASE COUNT        12280 a         7769 c         7762 g        12730 t
ORIGIN      
        1 aactaaattt caaacttttc aaagaactct tttagatttt aatgggacag taatgtagca
       61 gaatttaatg taattggaag gatttagata atcccgatat ttttcataat ctgaaggaaa
      121 agataacaag ataaaggtcg tttttattca aaaaacgctt tgcttttttg aataagcaaa
      181 gcgtttagct aatgaaatat caggttaata aatagctcat aaaatctctt ttcattcctt
      241 cttccatgct tttcaacagg ctaaattagc cacataaaat atttaaacaa tttttcaaac
      301 taaattgtat aaaattatga atttcaatgg tttaaaattg aacttcaaag gcgaactgca
      361 acttgacggt acttttaaaa aaatatatgc caccgatgct tcagcataca ggcaataccc
      421 ggttatggtg gctttcccgg cttgtaatga ggatatctct aagctcattc aatatgccac
      481 tgccaataaa ttgacccttg tgccccgtgg tgctggtaca tctcttgccg gacaggtggt
      541 tgccgaggct aatggtgttg ttgttgacat ttcaaggcac ttgaacaata tcacagaggt
      601 caatgtgcag gaaaaatggg catgggtaca accgggagta gtgcttgatg agcttaatct
      661 ggctatgcaa gcccatgggc ttttctttgg ccctgagaca tcaacttcca acaggtgtac
      721 cattggcggc atgatgggca ataatgcctg cggcgcacat tcgttgcttt atggctctac
      781 gcgcgaccat ctgttggaga tgaaaggttt tctttcggat ggttcttttg tgcatgttaa
      841 accgctcaat aagcaggagt ttctcgaaaa gatgaaacta aatacgctgg aaggggctgt
      901 ttataagcat atttttaagt tgttatcaaa tacccatact caaaaatcta tccaagaagg
      961 ctacccgcat cctttgctaa aacgccggaa cactggttat gcggttgact tgttgctcga
     1021 taatgaggtt tttggccata gccaggaact gttcaacttc tgtaagttgc tggccgggtc
     1081 ggaaggaacg ttgttctttg ctactgattt aaaaatcaac ttgttggagc tgccccctaa
     1141 acataaaggc ttgttgtgcg ctcattttca ttcgttggat gaagcggtgg aggctaattt
     1201 gctggcgttg aagcaccaac cggcttctat cgagctgata gacgatatta tcatcaattg
     1261 taccaaagat agcataggcc aaaaacctaa ccgctttttt atcgaaggca atccgggtgc
     1321 aatcctcatt attgagtttt atgctgagtc ggaggttgtg ttgcagcaaa tgcaaaggaa
     1381 tcttgaggct gaccttcgtc aaaacaacct gggttttcat tttccgtttg ttacgggagc
     1441 tgatatgtct aaggtctggg cgatgcgcaa agcagcgtta ggacttttat caaacattgt
     1501 tggagatgcc aagccggttt cgctggtcga ggatacggct gtttttcccg agttactaaa
     1561 ggattatctg cacgagttta aagcaatgct tgcaaaatat cacctgactt gtgtttttca
     1621 cggacatatt tcaactggcg agttgcacct gcgcccggtg ataaatgtca aaacaccaga
     1681 gggcttgcaa cttttccgaa aggtggccag cgaaacggcc gacctggtaa agaaataccg
     1741 gggctcgctc agtggcgaac atggcgatgg ccgtttgaga ggggagttta ttagtaagat
     1801 tattgggaag gataattttg acttgtgttg ccagataaaa caagcctggg acccccgtgg
     1861 tattttcaat gccgggaaga taacggctac tccgccaatg gatactaatt tgcgttatgt
     1921 ggtcaatcct caattcgata atataccgac ttatttttct tttgacgatt cggcaggcta
     1981 ttaccgtcat atcgagcgtt gcaacggctc ggccgactgt cgtaagggcc aactgatggg
     2041 ggggactatg tgcccgactt tcatggccac aggcgatgaa ttgatgtcca cccgtgcacg
     2101 ggccaatatg ctccgcgaag cgtttcaaaa ctttgatggt atttggaata cccaaaagga
     2161 tactttggag gtattggaca actgtctttc gtgtaaggca tgcaaaaagg aatgcccgtc
     2221 taacgtggac atgaccaaat tgaaggctga atacctccag catcattacg atgctcatgg
     2281 tgttcctctt aaaagcagga tgattgccag cctgcctgtt ttatttaaac tcagttccct
     2341 gtctccggct tttttcaatt ttatttcgca aagctttgtt ggacatcggc tcctcaaacc
     2401 tttattgggc tttagtaatc agcgttctat ccctgtggtt tatcgtttta ctttaactaa
     2461 ttggattgac aaacaaaaaa aagcggaggc aaaaaatata aaaggtaaaa acaagatttg
     2521 gctttgggcc gatgagttta ctaattacct cgatgtggaa gtggggatga aggcttataa
     2581 attgcttagc ctgctgggct acgatgtgca tctgttgcct atggtggcga gtagccggac
     2641 ttacctgtcg aaaggcatgg taaaaaaagc caaagccatt gtcaataaaa accttgcctg
     2701 gtttgaacgt cattatgacg ggatgcctat tgttggtatc gagccttcgg ctattttgac
     2761 cttgcgggac gagtatctcg accttgccga tgcctgctat aaaagcattg cacaaaaagt
     2821 ggcccaacat tcaatgcccg tagaagagtt tatttgcaat ttgtatcaag cccggcctga
     2881 aatatcgtct ttatttcaag acaaggaaat tcatattgcc ttgcatggcc attgttacca
     2941 aaaagtattg tcggatacct ctatggtcaa aaaaatgctc gaaattccgg ctaatagcaa
     3001 ggttaccgaa ctaaaaaccg gatgttgtgg gatggccggg gcgtttggct atgaaaaacg
     3061 ccattaccag ttgtccatgc agataggaga gttgagcctg ttcccttcgg tacgcaaatt
     3121 aagttcagat acaattgtgg cagcatcggg taccagttgc cggcaacaaa ttttggatgg
     3181 caccggtaaa aaagcttttc acccggtcga aattttgtat aatttactaa caaattcaca
     3241 tttgtaaatt acaaaaatat actttcatca ataataatta gatacatttt gtttacaatt
     3301 gtatatttat aaacatgtaa atttaccttt aatttattta tctataaatc aatgctttat
     3361 atgttgttaa ttaaacttta tttacaagta aaaagacatt ttaagtttat tgtagtaaaa
     3421 taacattata aaaacatatt tatgatgtta taaaccaatg ttttaaatta aatataatca
     3481 tgcattgctt taagttgttt ttgattataa cttattaaag tcttttaaat tcaataattt
     3541 taagcgatta tttcttgttt tttgggttat tgtataggca tatcgcatat ttaagtgtta
     3601 ttcatttgct tagctagtag tttgtaaact attgacttac gccctttatc ttatcgccgt
     3661 caagctaagg aaaagcccag tatgggcgac atattggtag aaaagagtct gataaaaata
     3721 aaacgcacca agcacaattc taatacgaag atctattgtt gtatggtgcg taatatgaaa
     3781 tgtatgttaa atgggtattt ctattgattc cccaaaaaga atgcactttt ttatcatcct
     3841 tctggtgcta tttattgggg attaaagaaa ttctttatca gattaataat gtcagaccat
     3901 ccttcaagtt tatgcgctat atctaaccca ataatagaag aaaccataaa aatattatca
     3961 cggattaaat aggttattta taagtcaata aatcattgaa ttgcaatata ttcaaagtct
     4021 catgcaactt ttttatcgaa aaaagttgct caaaaatttt tatcgcctta actctttcat
     4081 cctcgaagcc tgataatttg caaacaacag gtgcgttagg cttctcggcc gaaatcaaac
     4141 aaaaaggcga tgaagacgtt attttcgcta ttcaaattag cacctaaaca acattgatta
     4201 atgttttaac ttattgataa taagcgtaac tatttatatg tttttagtaa tccaaaagtg
     4261 atatagtttc tgccttgtaa tgcccaaaat gatgcattta ttactcttaa acccaactaa
     4321 aatttttaac atggctttac atttgtaatt tattcaatta caaaattaat ttgcatttgt
     4381 aaacaatttt acattacatt tgtaaatgta aaattgccat tatgcaggag cgacttgtac
     4441 aattaatgca ggccaaacaa cttaatgcag ctaagttggc cgacttatta ggtattcaac
     4501 gttcgggctt atcccatatt ttatcgggta gaaataagcc cgggtatgat tttatggaga
     4561 aattattgct taaattccct gatttaaatg ccgaatggct tattttaggt aaaggaaaca
     4621 tgcttaaatt atctgttaat cctattcagg atattttttc ggccaataat gaggttaata
     4681 atgctgctaa tgttgttaat agtgaagagg ctccggtgta taatactatt caaaaacctt
     4741 caaaacctgt caatcttgac aaggttacag atgtaaattt attgaaaaac gttgaaaata
     4801 aacaaataga acgcatcgtg ttgttttatt cggacaaatc gtttagcgaa tattctcctg
     4861 agaaataatg gcgatgtttt gtatctttgt caaaaaattg ccatgtataa aacaatcatc
     4921 cgtcctattt tctttttaat caatccggaa cgggttcatc acattgttgt tactttagtt
     4981 tcttctgtca ggtttattcc tgggggaaaa tggttgctta aaaaggtttt tgctattaaa
     5041 acgcaaccaa ttaaagttgt cgggattgag tttcctaacc gggtgggact ggctgccggt
     5101 tttgacaaga atgcttcttg ttttccttat atggccttgt ttggatttgg ccatatcgaa
     5161 gtgggcacgg ttacgccaaa aggccaaccg ggcaatgaaa agccccgttt gttccgctta
     5221 tgtaaagaca atgccttgat taaccgcatg ggctttaaca atcatggtgt ggatgctgct
     5281 gttgaaaaac tcaagaaact tagacgtgat ggcgtggtca ttggtggcaa tataggtaaa
     5341 aacacggcta ctcctaatga agatgcggta ggtgattatc tggactgctt tattaagtta
     5401 tacgaccatg tcgattattt tgtggtaaat atcagttgcc ctaatgtaac caacctgcat
     5461 cacttgcagg ataaggattc gttgtcggca atccttgggg ccattgtcga acaccgtgca
     5521 aagcaaatta cttttaagcc tattttgttg aaaatttctc ctgatttgaa ctttaatcaa
     5581 atcgacgaca cgcttgaggt gttaaagcaa tataacattg atggggtggt cattagcaac
     5641 actacggtta cccgtgataa cctgattact gataagagca tagttgaaaa tattggacgt
     5701 ggaggattga gtggcaagcc tattaaagag cgctctaccg aacttatccg ctatgttgcc
     5761 caaaaaacaa accatacact gcctatcatt ggtgtagggg gcattatgtc tgctgccgat
     5821 gcacaggaaa aaattgatgc cggggcttct ctggtgcaga tttatactgg ttttatttat
     5881 gaaggccctt ttttggtaaa aaggattaac aagcaacttc aataaaacgt tttagcgcaa
     5941 tatcagtttt gttttgtaaa ctgtttaaac ttgtaataaa aatccttgtt gtccggtaaa
     6001 aacagctcgc tgctatgcgg catagaaaca attggcgtat taggttatcc tacaaacagt
     6061 ccacagctaa gctgctatta ttttgtcgcg tagcgatgtc ctgtttgtag taaaattaaa
     6121 catccttaaa attaagcccc gccggggcga cctgtttcta atcaacgata ttctagtatt
     6181 tataagtatc tatttttgag tgaagtcgaa aaatgaatat ctatactttt tacaccctct
     6241 ttaatttatg ttggcttaac tttcttctat ttcaatataa ataatctcat atcctttcat
     6301 tacttctttc acagcataaa taatgcctaa aaatacagaa agcatgccta aaagaaagaa
     6361 caccactacc aaaccggaaa tatcaaaatt gttaaataca atattcaatc caatgcccaa
     6421 acaggtgata ataaataatg ctacggcagt actataacaa acaacagcgt tttttaccat
     6481 ctttatcctg aaggccagct ttcctgtttg cctgtctata ctgttaaaac gcttttcctg
     6541 atgggcattt agactatttt cttctgccat ttgtttgagc ttgcgttttt cttcgtccag
     6601 ggccctgata cggtttatta ccatcgaata tttgttgttc atgcccagga ttaaaaggcc
     6661 acaggccgat atcatcactg ccggggccat catactttgg ataatttcaa ctactgtcat
     6721 tgcgttattt gttttgtggc gattatccaa atgttatgcc attatgtgct tgcgatgatg
     6781 taaaacacct gttttttcta tttgctataa agaatgtgcc aattggctta aattttgttg
     6841 agtgatttta attaaaggac ttttatatcc ctattatatt ttagtatttt tgtaaaaaac
     6901 aatcgtaatg gtttcattta atgagaaaaa taccacggca ttgaaccatg tggtaaaaga
     6961 attgtcagac ccaaaatcgt atgagctggt gtaccacaaa ccacagctca acgaggagat
     7021 gatttctgtc gaggctatcc gcgagattgt tgagttggcc aaagaaatta tttttccggg
     7081 ctattttggc aataatgctg tacgtgccga cagtatcgac ttttacatgg gcgtttcggt
     7141 agataagctt tttaagttgc ttgttggcca gataaaaagg gggtattgtt tttcgtgcaa
     7201 tgaagataac tgctcagatt gtaataattg tgtggttgat gccgaatcta ttgccttgag
     7261 ttttattgaa tctttacctg ctatcaggca aaagatggcc aaagatgtac atgctatgtt
     7321 cttgtacgac ccggcatcaa agggttatgg cgagattatt tttgcttatc ctgctattat
     7381 tgctattacc cattaccgca ttgcgcacag gttgatagaa cttaatgtcc cgattatacc
     7441 acgtatcatc agcgaaatgg cgcactctgc aactggtatt gatatccacc cgggcgctgt
     7501 gattggcgaa aactttgtta tcgaccatgg aactggtgtg gttattggcg aaacttgtat
     7561 tattggaaat aacgtcaggt tgtaccaagg ggttacgtta ggagcccgca gctttcagct
     7621 cgacgaacat ggcaacccgg tcaaaggcgt ggcccgtcac ccgatagtcg aggacgatgt
     7681 aattgtttat tcgggagcta caattttggg aagggttacg ataggcaaag ggtcgattat
     7741 tggcggaaac gtttggctta cacaaagtat ccctgctaat tcgcggatat tacaaggaaa
     7801 acccagagaa actttatttg atatgggaag cggtatttaa actttgccgc tttcttcgtt
     7861 tttatacccg tagatataca gcgaatgatg cgatgggatg aagttgttca tctcgcaggc
     7921 agtcttgata atctgttgta tgcgtgggtc gcaaaattca attaccaggc cgctgttgat
     7981 gtctatcagg tggtcgtgtt gcaggcactg aaaggccctt tcaaactggg caatgttttt
     8041 tccaaactgg tgcttgacca caaggttggt ttccaacaaa agctcaatgg tattgtacaa
     8101 tgtagcccta ctcaccctgt aattcttgtt tttcatggaa atgtaaagcg actcaacatc
     8161 aaaatgcccc gaacgggagt atatctcgtc aagaatagca tacctttcgg gagttttgcg
     8221 gtgccctttt tgctctaagt agtcggtaaa tattttcttt acggtttctt ttacattttc
     8281 ggtcttcata tttagactaa ttaaaaatta ctgtaaaagt atattatttt ttaaaataaa
     8341 aaaaccggct ttaggtgccg gttttcatta tttatcattg aattacttca aattttcaag
     8401 cgctgttttc aaacgtttta aagcttcaac aagtttctca tcggaagtag caaatgaaat
     8461 tcggatacat ttgtcattac cgaaacctga accaggaact gtggctacaa aagctttttc
     8521 taacaggtaa aaactcatgt cgtcagagtt tttgatagtc cttccttcgg cagacttacc
     8581 aaagaaagcg ctgatatctg ggaatacata gaacgcgcca tctggtttgt tgcattttac
     8641 acctttgatt tgcatgatag cattgtaaac caaatcgcga cgtttgtgga aagcctgacg
     8701 gaattcagca actacagcct ggtcgccatt taaagcagcc gcagcagcct tttgggcaat
     8761 ggttgaagcg ttggtggtca tctggccctg gagcttgttg caagctttgg caatccaagg
     8821 ggcagcagcc ataaaaccaa tacggtaacc agtcatggca tatgattttg aaacaccatt
     8881 gatgatgacc acgcggtctt taatttcggc aaactgggca atgctttcat gtccgccaat
     8941 gaaattgata tgttcgtaaa tttcgtctgt gataacgaaa atgtcaggat gcttaaccaa
     9001 aacatcaacc agagccttta attcagcttt tgaatatacg ctacctgttg ggtttgaagg
     9061 agagcaaagt aataaggctt tggttttgcc ggtaatggca gcttcaagtt gagcaggggt
     9121 aatcttaaag tctgattcta cttcggtgtt gataatgacg tttgttcctt ctgccaattt
     9181 taccagctca gcataactta cccagtaagg agcagggata atcacttcat ctcccgggtt
     9241 gattaaagtt aatatggcat tggccaagct atgctttgcc ccattcgaaa caataatttg
     9301 gttgggagca tagtccaggt tgttttctct ttttaacttt tttgaaatag cttcgagtaa
     9361 atcagggtaa ccggcaacat tggtataaaa agaaaagtta tcatcaatag ccttttttgc
     9421 tgcatccttg atatgcttag gagtgaaaaa atctggttct ccaacggtta agttaattac
     9481 atcaatccct tgtgcctgca actcctttgt tttggtgttc atggcaatag tagaggattc
     9541 agctaaagct tttactctgt tcgatacttt ttccataaaa ttattaattt gttttattaa
     9601 ccgtgcaaaa ttaaactatt atcttagcat tttcaaacat ttagagaatt atgggcaaag
     9661 tttattgcat aggggaaacg gtttgtgata ttattttcaa aaatggcaac cctgtagctg
     9721 caaaagccgg agggtcgatg ttgaacagtt cggtgagcat gggaaggaca gggttgccgg
     9781 tttctttcat cagcgaatat gccaacgacc agcttggaca gatgatagat gattttttga
     9841 aagattcggg cattgatacc agcaatattt tcaggtatga taatgggaaa acctcggtag
     9901 cccttgcttt tcttaacgaa aagaacaatg cctcgtacag cttttataag cacttgcccc
     9961 aggagcgttt ggccattcaa atgcccgact ttactgaaga tgatgtgctg ctgtttggtt
    10021 cgttttatgc tattaataaa gaaatccgcc caacggttgt ggaacttgtg accaaagcca
    10081 aaaaggctgg cgccgttatt atttacgacc caaacttccg caaagcccat ctgcacgagc
    10141 ttagcgacct gatgccttat attattgaaa atatgcaaat ggccagtatc ataaggggct
    10201 ccaatgaaga ttttgaaatg atttttggtt caaaaaccat tgctgacgct tataatgaag
    10261 taagcaaata ttgtaaaaac ctgctttata ccgaaaatga aaaaggcttt tatatacaaa
    10321 ccccgtcagt agggacgttc atacctgttg atgctattgt accagtaagt actattggag
    10381 caggggacaa ctttaatgcg gggtttattt acggcattta taaagcaaaa gtaaagaatg
    10441 cagaaataga acgctttaca tcaagccaat ggaatgaaat ctgccattat gccccgattt
    10501 ttgcaaaaca ggtgtgtaaa agctatgaca attacctctc aaaagaattt gcagcaagct
    10561 ataagctgta aaaattaacc gttgaaccca acgctacgtt tgtaactggt agcagcgttg
    10621 atatagaagg ctttgtattt aatgtaatgg ttaccttttt taaattccca aatgacattt
    10681 ttggctttga aaattttgcc aatacaggct tcgaaaatca aactataatg aatcccagta
    10741 gctgctgaag cttctatgat gcttttaaat tcaatgcttt tgtttgaaaa atggccaatt
    10801 acaggaatat gatatttttt gttcatcaac ttattctctg ttttttcatt aattatgcgc
    10861 agcttgccaa tatttttcat agcctttatg catttggttt ttacaaatat acatctttat
    10921 ctgattaaaa acaacatcaa ataatgataa actgatttag gtagaatgag cctcataatt
    10981 attcactgtt gtttaaaaca aaataacaat aaaaatgtgt cgccccaaaa gagcttaata
    11041 tccttgtaac aacttgacta ccatactttt gtggctaata ctactaataa tatgtcctgt
    11101 aagggcagta gtatggcaga agtgttgaat tatagttaaa ataagctcca taggagcaac
    11161 acctgtctaa aataaattta ttggttagct aacatgaatt tcaaaattga actgccgcaa
    11221 ttaaacagaa atttttttaa ttcatcttta tattggccca aacattaaaa aaggctcaga
    11281 aataatcctg aacctttttt tgcaattatt gtcttaggta acctttgtaa atcattaata
    11341 attaaagtcc taaagattta aaaaaagcat cctggtttgg ttctctgccc aagaattttt
    11401 tcacgatgtc catttcattt acagttgatc cttttgagat aacttcattt ttgtatttca
    11461 atcctgtaga gggatttaag actccttctt tttcaaatac agaaaataag tcctctgcat
    11521 acacctttga ccacatatag ccataataac ctgcaccgta gcccatcagg tggtcaaaag
    11581 aagcctgcat gtgggttccc ggaacatact caaacaagga aaccttatta ttcagctcaa
    11641 tcatagcatc ggtggtagtt tgagttccat cagggtcgta tttgtcgtgg taggtgaggt
    11701 cgagcattcc atactgcaat tgcctgaggg catccattcc cgaacataaa ttgcgggccg
    11761 aaagcatctt atcatataac gctttaggca gcacctctcc ggttttatag tgtttggcaa
    11821 acaagcaaag cgattgatag ttccatgccc agttttcaaa catctgtgac gggacttcga
    11881 caaactcgtg ggtattgctg atacctgcct gaaaagacaa gtcagagcgg ctcatcacaa
    11941 aatgcatcat gtggccaaat tcgtggaaga atgtttccac atcgtcatgc gacatgaacc
    12001 cgggcatatc tgctgttgct tgaggaaaat tacaaaccag ggcagcagta ggtatttgat
    12061 aaccattggt agtttttttg ccgtttatca tcccaaaaca agcaaaatgg ctgtatttgt
    12121 cagggcgagg gtagaggtcg aggtaaatac gtcctaccac tttagtccct tctttcattt
    12181 caaaaagctt aacgcccggg gcccaaacag atgcaccttg aacttcttca aaagaaacct
    12241 gatacaacgt tttacaaata gttaatatac cctggataac attattaact tcaaagtatt
    12301 gtttaacaag ctggcggtca acgttgtact gttctttcaa cactttattg cgcaaataag
    12361 cggtttccca agggtttatt ttggttgctt ctggattgtc aagttttttt ctgtcaagca
    12421 attgctgata atcttttaaa gctttaggtt taattttgtt atataacgaa ttttcaaaat
    12481 cccaaaccgt agcggcattt ttagccatgt tatcggcaat aatataatca gcagcagatg
    12541 cataacccag caacctggca cattttttac gctctttcaa cactgcttgc aaaatgtcga
    12601 ggtttttgtc agcagcacgg tttgaatata agatagaaag gcgtttacgg gcatcatcgc
    12661 tttcagatag ctccataaaa gggactacag aagggtggtt aagctctatt ttatatttcc
    12721 cgttttcttg caaacgggct tttttataat cctcaggcaa cccttttatc tcgtcttcag
    12781 tcacctcaag ataatctttg caggcagcaa tattggccga gaattgcaag cttaaggtgg
    12841 aaatactgtc aagtattact ttaagttctt tacgcttgtc ggcagataaa tcaaagcctt
    12901 gaagatgata aaaaatgagc atatcctcca gtgctttctt tttataacct gtcagttttt
    12961 tagcttcatt agttgtagaa taagccttga cagctttata caattcctcg tttaaattga
    13021 cttcgttgtt aaatttgctc aacacttcaa tgtttttctg gcatacttca cgtaaactat
    13081 catcagggtg ggtgtatgcc atcaggtaaa tgatattggt caggctgctg aggcgctgct
    13141 gcatatcatc gtagcgtacc atagtatttt caaaggtacg tttactatta tccactgcgg
    13201 caatttcagc aatgtacttt tttacatctt ccatcaccag ctgagtggca gattccacct
    13261 gcagggcagt aaacttatca aactgtatca aatcgttttc tttaaataac aatgcgttgt
    13321 ccatagattt tgtctgaaaa ttggtaaaac agagcgcaaa agtaagcgaa taaagtattt
    13381 ttttcatccg taaataattt tataaataag gtcggatgaa ccccgaaggg ctctccgaag
    13441 gaaaactcgc atgatgaatt ttcatccttg tttgtgttta acgaatcatg ctcgtttcat
    13501 attggttaat ttaataatag ttatgtaatg aataaataag ggtgcaatat ttgaaaagtt
    13561 ttcctttctt ggaagttatt taacaagaat tttttgtaat agtttaagcc ttgttggcac
    13621 ttgttaaatg caaggcgagt aggagagaat aataccgggt aatttcgtta tctgcccggg
    13681 aagttaacac aatgggcact ttggccccgg caacaaccgc agctacgggc tgacctgcaa
    13741 tgacacctat agacttatac aatatattcc cggcatcgag attgtgcacc actaaaacat
    13801 ctgcattccc ggccacttca cttttaatgt ttttcatttc agatgcagcc gccgaaagtg
    13861 cgttgtctaa agcaaaaggg ccatctacca tgcaattttt gatttcacct tttaggttca
    13921 tatctttaag aatcagggca tcttgcgtag aggcaatttt gggattaggc ttttcaacag
    13981 ggcaaaccat ggcaacttta ggatgtccat aaccaagttt tttcatcaaa tcaacggcat
    14041 tgttgataat agccacttta gtttcaatat ccggggcaat gttaatggct gcatcagaca
    14101 aggctaccaa ttttggttgc aaaggatgtt taaatatagt gatgtgggta agaatgttct
    14161 ctaataaagt actgtgcaat atagctttta caaaaatact ggtcgaaacc atacctttca
    14221 tcaaaatacc cacttcatgg ttttcggcca taacaacagc atcatgggct gctataacct
    14281 ggtcattgct atggataaca gtatattgca atggcttaag gccataagca tggcacaaat
    14341 tttctatctc aggtttgttc ccacaaaaaa cggggataat ctgtttagct tcaataaaag
    14401 gtttaacagc cttaaaaatg ccggcatctg taggccagac aatggctgct ttttgtgggt
    14461 agttgcaaac agctaaaagt tgatcgaggg cagttatcat acttttttta gtaaatgtaa
    14521 tcactaccct cgatttattt aaaaaaattc tttaagaatc tttgctgcaa agctaatatt
    14581 aatgatgaaa caaccttacc ccggtgttaa tgatggttat tcccagttta ttggccgttt
    14641 caataatttc attgtccctg accgagccca ttggcgtggc aataaacttt atcccatatt
    14701 tgtgggcaag ttctatgtta tctttttgcg ggaaaaagcc atcagatagc aaacaactgt
    14761 caggcaactg gttaagtatg attttatcgg caaatgtttc ttgtcttttt tgctctataa
    14821 tctggtcaat atcagtgcgt ttcagccctg tgtaattttt taaaaaagaa tagtcgagca
    14881 acattttttg gtaccagaca ttggctttgc tcaatgctaa tcctgaacat aaaatacgcg
    14941 attgttgtcc ggagccaata ccaataacat ggccattatt aaccacacaa attgaattag
    15001 attgggtgta tttcagggtg ataaggccca gtttcaggtc ttcctttatt tgaggagaaa
    15061 tagctgcatt tgaagtaaca acattagaaa gtaaagcatc ggaaataacc aggttattcc
    15121 gtttttgtct gacggtaata ccaaaaacat cgcgcgattc gatttcctga ggctcataag
    15181 ctgcatcaat ttcaaaaatt acatagccgc cgtttttttt gccgcataaa atctctaacg
    15241 cttcttgggt atatcccggg gcaacaatac cgtcagatac ttcagatttg ataagcaaag
    15301 ccgtttgttt atccaccaca tggcttaccg caatgaagtc tccaaaagaa gcaagcctgt
    15361 cggtaccacg ggaccttgca tacgacgatg ctaatgcaga taaatcctgg tttatcaaat
    15421 aagcctgttt ttctttttca tctaatggtt ttgaaacagc cacaccagaa ggagtgacgt
    15481 gtttgaatga ggtagaaact tctttgttta aagctttgct ggcctctttt accaattgcc
    15541 atgaattgag cgcatcaagc acgtttatca cgcttggcga gccatttttg agctgtaaag
    15601 catttttact atcaatcact tctgcataat cctggtaagg attcattcca tattttaatt
    15661 tcatccgtaa ataatttttt taataaggtt ggatggaact cgcatgataa atttcatcct
    15721 tgtttgtgtt taacgaatca tgctcgtttc atttttctat aatttgatga tttatttcgt
    15781 tttaaaacaa aaatagccga aacaacattg acgtttcgac tatttttatg ctacgtttta
    15841 ttttaccaaa gtttagattt tgaaacagca ttgcgctggt ttacaatatc tttagtatct
    15901 aatgctatta ccagttcttc attggtaggg acaacaatga ccttgatttt gctatcgggc
    15961 ttattaatga cttgttcttt acctcgggct gcagcattta atgtttcatc tatttcaagg
    16021 cctaagtatt caaacccttt acaaacttct ttccttgtca aatctgagtt ttcgccaaca
    16081 cctcctgtaa aaactaatat atcaagccct tcaagggcag ctgtataagc cccaatatat
    16141 ttacgaattc tataatggta catatctaaa gcaagttttg ccctttcatt gttttgatca
    16201 gcagcagccc ttacttcgcg catatctgat gaaactcccg atatgcccag gattccgcta
    16261 tgtttgttca aaatagtgtt taccgtaaat gcatcaacag cttctttttg catcacaaaa
    16321 tttattaccc ccgggtctac atcgccactg cgggtaccca ttatcaaccc ttcaacaggt
    16381 gtgaggccca tagacgtgtc aacagatttt ccgtctttga tagcagttat agaagctcca
    16441 ttgcccaaat gacaggtgat aatgcgcgaa ttgttataat ctttgcctaa tatttcgcat
    16501 gccctttttg atacatacaa gtggctggtg ccatgaaaac cataccttct gatgccgtgc
    16561 tttttataca aagagtaggg gatagcgtac aaataagctt tctctggcat actctggtga
    16621 aaagcagtat cgaaagtagc tacctgaggc acattgggca atatattctg catagcctga
    16681 atacctttga tattggcagg gttatgtaaa ggcgctaaag cagaatattc aataagtttt
    16741 tgcattacgt agttgtcaat gtaaacactg cctttaaatt cctctccgcc aagtactaca
    16801 cgatgcccaa cggcattaat ttcttcttct gtcttaatca cacctctttt aggactgata
    16861 aggatgccca taatatattc gataccagcc tgatggtcga gaatttcgcc ttcaagcttt
    16921 accgattcgc cattgtgagg ctcatatttt aaaaaagaac cgtgcaaccc aagtttttca
    16981 acacctcctt tggccatgac caactgtttt tcaatgtcaa aaagttgaaa ttttatcgag
    17041 gagctgccgc agtttaaaac aagtatcttc attttttaat ttatttcatc caacataaac
    17101 atttacccat gtgaaaaact aacggttaaa aagttcccgt gtcatcacat tgccggaaac
    17161 aatttttcca ttttcatatt tgtaaaaacc tttaccggtc ttacgtccaa gatagtttgc
    17221 cctcaccagc cttttaataa caggagaggc tttgaactta tacaaactat attcctgaaa
    17281 caaattatcc atccacttta ctactttatc aagaccaata cggtcggcca attcaaaagg
    17341 ccccaactgc atgccataac cttctttcat aatcttgtca attgcctcga ccgaagaaac
    17401 tccttccatc aatagttcac aagcttcatt gataagcgat acaataagac gggtacttac
    17461 atgtccgggc gattcattga tggtgataac ctggcggtca atcattttgg caaaggtaca
    17521 aactctgtta taagtgtttt cgtcagtatc gatacctttt accacttcaa caattttgat
    17581 agtggtagca gggctcataa aatgtaaacc aatagctctt tcaggttttt taagctcaga
    17641 agcaagctcg gttatcatca gggttgaatt gttgctggta atgatagctt ctggcgaaat
    17701 aaccgattct actttcctga aaatctcttt acgggattcg aggttggtat aaggcttttt
    17761 tgaattgatt gactcgatga tgatatcgca atccgaaaag tcgttataat ctgtagttcc
    17821 tttaatacga gatagtatcc ctttcttttc cgtttcggtc aagccccatt ggttaacctg
    17881 gtcgtccaat tgtttgcgga tactgttcaa tatttccgaa accctttccg gagaaatatc
    17941 cagaaacacc acatccattc caaactggct gacaatgcgg caaatttcct ggcccatttg
    18001 tccacagcca attactccaa tcttttgaat atttcctttc ttttgtgtcg tgcgtttata
    18061 cgcataatct tctaatactt cagccatatg ataagttgtt ttatttcgag ggggttattt
    18121 tccagcagcc tggtttgcgg taatggcaat aaggttgaca atgtcgctaa ccgagcagcc
    18181 acgagataag tcgttgatag gagctgccat accttgtaaa acaggcccaa tagcttcggc
    18241 atgggccaaa cgctgaacca atttgtaagc aatattcccg gtttcgagtg tagggaaaac
    18301 caaaacgttt gcctttccgg ctatttcact gcccggggct ttacttttcc caatagaagg
    18361 gatgatagcc gcatcggcct gcaattcgcc gtcaatcttt aggcttgggt ccatttcttt
    18421 tgcaatacgg gtagcattta ttaccttgtc gcacatctca tgttttgcac ttcctttggt
    18481 cgagaagctc aacatggcca cacgaggctc gaatcctgca attgccctag ttgttttagc
    18541 agtagcaaca gctatttcgg ccagttctct ttctgttggg ttagggtgta cagcacaatc
    18601 agcaaataca aacatgccgt cctctccaaa ttctttatct ttcagaataa tgataaacgc
    18661 tcctgaaact acgctaatgc caggtaatgt ttttacatat tggaaggcag gccttaaaac
    18721 atctccggta gcattcattg caccagctac ttctccgtct gcatctccgt ttttaatcat
    18781 cagagttgca aggtatagag ggttttcaat tagcttagca gcttcttctc ttgtaagacc
    18841 tttgcttttg cgtagctcaa ccatcatgtc gatgtaatgg tcttttttct catgctttag
    18901 cgggtcaaca atagttgcct tttctattga ttttaaacca aattgtttgg ctaaatcaaa
    18961 aattttgtca cggttgccta ttagtataat ttgtgccaac tcttcgttta gcacttcatc
    19021 cgcagcttta agcgttcgtt cttcaagacc ttcgggtaat acaatgcgct tttggtgttt
    19081 tttcgcattt gcttttattt gttgtaataa atccattgta aatcaatatt ttgggtttca
    19141 aaattttaaa atgtaaaggt agggtatttt cccctttgcc tatcaagaaa agagaagaat
    19201 ttttttgtca aactaacaat aaaagtctgt cagtttctca gcaatagtta gaccgatgga
    19261 aattcgtcaa atacaaaacc atgattatta gcccatttaa cagctatctc gcaattgtaa
    19321 tttgctaaaa tattgctatt tacagtttta tcaatttgac aggggacaat acccaggttg
    19381 ttttttaacc cttcgctcac ttttttatgg ttatgctgga taaaaaacca tgcattggct
    19441 ttagatacca caatatcagc tcctgtatga agctcattat catcaaggcg tgtaggaaag
    19501 aggtttttgg gagataacca caacgattga tgtttttgcc cgttttgcca tagcagatat
    19561 tgatttttaa cacattcaaa tacttcatca ggggtaacgt aggaggtatc aagcaccaaa
    19621 tcatagttgt aaatatcgtc aatgtctaca ccatactgct ttttaaaacg catcacctcg
    19681 cttttgcggc gctcgtaatt gctgcggatg gtatcttcta tgctggcatg tttctcattt
    19741 tcgcgaacag tgtcgccaaa tattcttttt ccggcaatgg ttttgcttac aatcaaatga
    19801 attttaaatg catggggtac aaaatgccag gccagccttg agtcaagaat tagtggttca
    19861 tctgattttc cataacggct aaccatatta tcgcactcaa tatcaatttc ggggtgggtt
    19921 tccatgtatt tattaaaggt tgtggtatcc atgccatatt gcatggccag tttgcgctga
    19981 atacccccga cagaaagtat ttcgtaagat aaatacgctt gtaatttttt ggcaagcaca
    20041 gttttgccgc ttcccaaatg ccctgatatg gttattttat tttttagcat acgatgtttt
    20101 tcaaagttgc aaagatagga gatatatgcc tatttttttt gtcctatcat ttctttataa
    20161 acaatatcct gaatttttgt cctgacgttc atttcggcca gcttgttttt ggcagctgaa
    20221 ggatacaccc ggttagaaac aaatatataa actaagttat attgaggatc aacccaggtc
    20281 atcaccccgg taaagccggt atgtccaaaa ctctggtcgg aacaataacg gcttaccgga
    20341 ctttgtttgg cggtatcagg ctctggtttg tcaaagccca gtccacgtct gttacccgca
    20401 aataatcggg aattgaattt gttcatggtt gaatcctcga aatatctgtc gcctccataa
    20461 atccctttat tcaaaaatac gtgcatcaat ttgcccaggt cattggccgt tccaaacacc
    20521 ccggcatggc cgcatacacc accatacaca gctgcaatgg ggtcatgcac gtatccttgt
    20581 atgagctgtt tcctgaaaat ggtatcattc tcggcagggg ctattttgct ggtgtccatt
    20641 tttttcaatg gtaaatatcc taaggttccg gcacctaatt tattataaaa aagctttgaa
    20701 gaaagaacat ccagttttga ctttgaaata ttttcaacca ctctgcccag catgataaag
    20761 ttcaaatcgc tgtagttata aggattattc tttgctaatg gagtatttat gatttctttt
    20821 tctattgtgt cgttccagtt tttgaaagta taaatacctt tggccacttc tacagcataa
    20881 tatttgtctt tataattggc ataataaacc ttgttgagct ttgtagagca atttacagca
    20941 tggctattgc cttttgtcaa cctgctctta tctatacaat tcagcccatt taaatatatg
    21001 gtggaaaaag atttaaaccc gcttcgatga gtcattaggt cttgcactaa taaatctttg
    21061 aacccttttt tcttaaaatg ttttaaataa tgccccaacg actcgtccaa ttttagtttc
    21121 ccggcctcgt aataatgcat taacaaaggc gttgtcgcgg caatcttggt aatagatgcc
    21181 aaatcataca cggtattggt cgaaacagcc agggtgctgt cgtaagtata atatccaaat
    21241 gccttgtgat aaaacacttt gttgtctttg gcaatataca caaccccgcc cggaatggct
    21301 ttttgtctta tggcatcgtt catgatagaa tctatcgggg caagacgttt tgaagaaatg
    21361 ccaacttctt cgggtatggt atattttacc ctgatagtgt tgtcccaggt aataccagtg
    21421 cctaatttcc atttttcatt aatcgaaaca ggcaattttc cctgggcttt tatagcccca
    21481 aaaattagtt gtgcagaaag tgcctgggtt tcctttttat catcataaga aataagaata
    21541 gactggaagt ttacattttc cgggaacctg ttcatcaggt atggcgaacc aaagatatcc
    21601 attatcaatt gacacgaatc agccaaaaag ctaaaaaagt tgatagcctt gtctgtaacc
    21661 ccgtaattct tgcttaagcg catatcggta ttgacaatgc tacctataac aaggttatat
    21721 tttctaagcc tggaatacat ttgcaccagc acgctgtctg atgtgttttc atttacataa
    21781 tactcatcaa taggggcata taagccaagg gtctgttgga atacacaact tgtatcgcat
    21841 ccccatgaaa tggaagctat atagcaggta tctaaatgct ttacaggcaa aagatttttg
    21901 ggatcatgaa taatggtaat ggctttttca atctcatacc ttgacaacag cctgtataaa
    21961 ggttgttgca aatcagcaac aacattattt acagagggtt tttggcgcga cttatctaac
    22021 ccaagccaaa ctttagcttt taaaattttt ttgcaacgtg aatttatttc atcttcaggt
    22081 acaactccgg ctttaacggc tcttttgata gtatcaatag cgtcttttat attggcaggc
    22141 ataagcaata tgtcattccc ggcaataact gcactgataa tttgttcttt ctcttttttc
    22201 ttttgggccc ctttcatatt taaggcatcg gtaaaaatca accctccaaa gccaagctgc
    22261 tttttcaaaa tatctgtgac aatgataggg gagagggacg ctggtttccc ggttgtatcc
    22321 agcgatggca tgttgatatg gcctgtcatg acagagctga ggccattttt tatcaattgt
    22381 ttgaaaggat aaagttcaag gctgtcaata actttggctg gtttatttat aaaaggcaaa
    22441 tccaggtgtg aatcagtccc cgtgtcgcca tgtcctggaa aatgtttggc tactgccaaa
    22501 attcggttat cttgcaaccc tttcatataa gccatagcca ggcgggtcac gttttcgcgg
    22561 ctttcgccaa acgaacgact gttgatcacc gggtttcgcg ggttattgtt aatatcggcc
    22621 acaggggcaa agttggtatg taaccctaat ctgcgaagtt gctgcgctat atcgcaagcc
    22681 atctggtaaa tcaaagcggt atcattagca gcgccaagca tcatttgctt agggtacaac
    22741 tgggcgctat caaagcgcat ggtcagtccc cattctccgt ccattcctaa tatgagcggt
    22801 gtacgtgcag catcctgtaa cttgttgacc aatcgggcca cagtggtgac accgccctga
    22861 aatataatgt acccgccaac attatattta gcaatattga taagttgttg cgatatgttt
    22921 tgcgtgtctt tgggatacac tgcaggcata atcatctggg ctatacgctc ttccagagtg
    22981 aggcttttaa acacagaatc aacccattta tcagaagtat tatcgatgac agaaggatat
    23041 gtttttatct ctttatcaga ttgtttggca atgacaaata ccgaaacaca tagtaataca
    23101 aaaactattg acttacctcg cataaaaaac taacactaac attacttttc aaaaataggg
    23161 tatcccaaca ttaaacacga caaagcttat aaacatttta taaacaatat gctgctaaat
    23221 attctctatg tagtttgctt gtattattta tttttgcaaa aaagtcattt atgttatcca
    23281 gcgaagtaaa aatcagggtc agatacggcg aaacagaccg catggggtat ttgtattatg
    23341 gcaattatcc gttgtattac gaagttggcc gtaccgaact gctcagaaaa catggttttg
    23401 cgtacaaaat tcttgaagat tccgggatat tgttgcctgt agctgatatg aaggtcgaat
    23461 attattttcc ggctttatac gatgaagagt tgaccgtgaa aaccagtatt ttgaaaaagc
    23521 ccggggtaaa aattgagttt ttgtacgaaa tatttaactc agaaaactta ttgataaata
    23581 aagctacaac aacacttgtt tttgttaaag catccgacag aaaaccatgt aaacctccca
    23641 aggatttttc ggatttggtg gacagatttt tttaaaaata acataagaag aatacttagc
    23701 atctgatatt tacagaaatt aactaaacat cccttacttt tgccttgaag caaaaaacaa
    23761 ttcaaggctt gccgtaaatt ggctaaaaaa agccttatct acggctccgg gcaacaactc
    23821 gcttcgtcga taaagttttt tcttaacgcc atttacgcca ggccattagc agagacgttt
    23881 caatgaaaca ggcgaagcat taaaagaaaa aattcctatt catccatcgc cttttgttcg
    23941 atttcggccg agaagcctaa cactactgtt gattgcatat tgtaacatta aaaggcttcg
    24001 agggtgaaag agttaaggcg ataagagttt ttgggcaact tttttcgata aaaaagttgc
    24061 atatcgaatt cgttataaat aacataaaaa tctgttgata aattgttcat aaaaaaacat
    24121 aaccagcctt ttactttttt cgtaaacaat gcccttcgca acaaacatta tcttgacaat
    24181 caatttttta taaaaaaaat acttaattta gggtaaaaac agtaatttct ggccacatac
    24241 caagccttgc cggaaagaaa gaaccacttt gaccaacatt aacataaagg tatctgtttt
    24301 catattggta taaaccgcac catgaatgaa tatttgacca catcaacggc gaccagggct
    24361 ttttgccatt tcgtttgcca aactggaaac catgagtatg ccctgagaaa gtaacagcca
    24421 aacgtgggat agtttttaac gagctatccc aaatagaagg gtcgtgcgaa agaaataaca
    24481 caaaagcact atccgggata ccttccatag ctttagccac atctccttta ctgcggaatg
    24541 gaggaattcc tatattttca atgcctgcta cccatatttt ttcattattt ttttcaataa
    24601 aaacatttgc gttgctgaga agtttgatat gggttttaag gtaaaaagaa tctattaacc
    24661 tcaaattaag ttctttgtct ttttccgaat gccatcgata atagtcgcca taatcgtggt
    24721 tgccattaat ggcaaatacc ccgtacctgg ctttgatttt tgaaaaatgg cctataaagg
    24781 gataagcttc ctgagcaaaa aaattcacca aatcgccggt aagaaaaacc aaatcaggct
    24841 gttgctcatt gaccaaagca atcatttttg ccagtttttt atcgtgatga acaactgtcc
    24901 ccaggtgcaa atcagatatt tgcacaattt tcaaaccttt gaattgattg ggaacagttg
    24961 tagatagtga ctgttcataa agggtataat taaaccttcc aaaccaatag ccatactgaa
    25021 ggtataagaa aaggacaaaa gcgaccgaaa aagatataca gataattatt tgcctgtatt
    25081 tctttatcaa cagcgagccc aacgacaaca gcacaaataa caatttgaag atatataaag
    25141 ggattagttg tgctgtaaat tcaaacatat tggctgtttt ttcagcattg agatatgaaa
    25201 cgtaaatgaa atagaaataa aacaagaaga acaaaaacag cgctgcaact gaaatatgga
    25261 tgataaagat ggttttccag tatttatgat gataaaaagc ttttttgagc agcaaaaaaa
    25321 tcagcaagtc aataatcagc attatcccgg caaatatcaa aaaactcaat accgggctca
    25381 tttgaaagaa atacaaaata ctcatagctt ttttaatttg ggctaaagta aagctaaatt
    25441 atataataaa aaaagtccgc taagggcgga cttgttatat atgacaattg atatttattt
    25501 attcatcatc atcagcatca gcttcaaaat cttcgctgtc agaaatatta tcatcaatat
    25561 caacagagct ttctggctca tcatagtctt tttcgtctat ttcgtcagga ttggtatcaa
    25621 tttttacagg aaccttcaca agataactta cttcaggagt atctaatgta acaccataaa
    25681 agaaatcatt ttttggtgta gtgactttaa tcacatggtt gttgtatcca tccggatatt
    25741 ttttgttcaa cagttcaagt agttccggtg ttaaattttg cagactaata actttgttta
    25801 cttttcccat aattcaatac taaaattttg tgcaataata aatcattcaa tgaaaagtta
    25861 agaaattttt ttgagttgat aaaaaaattt ttttaactat cttataattt atattatgtt
    25921 aaataatgta tataaaataa agaggctgca taatttacat tttatacagc ctcttatata
    25981 ttttaccaac ccatttcttt gtttatggca tttaatttat cagtcatttg catcctgaaa
    26041 tataatcctt tcagtgtttt ggcacagatt tcttcttttg ggtcaatagc atgtgctttt
    26101 tccaaccaag gaattgactt ttttaattct tcatttccct gaattgttaa ttcattatac
    26161 ttggcgtcgt ctttttcatt ggctgcttta tcaaaaatct ttacagcatt gttgtaataa
    26221 acaactccaa ggttgtaata tgcgttaaaa tttttaggat caatttcggt agctttggta
    26281 taagctgcaa ctgctttatc tatatcatta attttttcgt acaaagtacc ttcagcaaaa
    26341 taaaatgaag tattgtttgg gtctttttgt tttgccatat tgaggtaatt gagtgcttcc
    26401 tgggcttgtc ctcctactaa ataataattt acaaggtcaa caattaaatt aagatcattg
    26461 ggaaacaatt caaacccttg ttttaaaact gctaaagcct gcactgaatc accttttgaa
    26521 agatatgctg attttaagag agaatatgtt attcctccgt tatatttata atcggccgct
    26581 tttttgaaat attcaatggc tttgtcataa tttttggcat tgttagcagc aataccgcag
    26641 ttaaatatca ttgaggtgtc aatgaaattg ttaaaaaccg gatttttgta tatttctaaa
    26701 acaagctcaa aatactcgag cgatttggaa taattttgaa ccccataggc atcgcttcca
    26761 taaacagctg ctcctctggc caattcttcc atttgcatgt taattttttt atcaaatttt
    26821 ttcttaacat caatttcgtt aactttttta taagtttcaa aagcttcttt tggcgcattt
    26881 tcaaaagatt ttctcaatga agaatcaggt gaaattgaaa ttttttgtaa aatctgagct
    26941 ttaacaaacc aagtattagg caaatctttt gtttcatcat tggtcaaagc tggttgaata
    27001 gtttcataag ctttagccag ttcatttttt tccattaagc tctcagcatt atttactact
    27061 ttcttttgtg ccgaaagaga aataaacata gccgctgtca ttaacaaagc aagtactttt
    27121 ttcatcactt tttcttattt aattgattaa catttaatat tttgcaaata aaacaatttt
    27181 tactgtaatt tttattcagc aggaagattt tcttctccta cattactttc ttcaacgccg
    27241 ccttctacca caggagtttc ttcatcctgg cttttaggga cctgggccac agctgctatc
    27301 gaatcgcttc ccttgatatt aattaatttg acaccttgtg tagcgcgccc ggtgacccta
    27361 aattctgcaa tagacaagcg gatggtcaac ccgtttttgg taataatcat caggtcttca
    27421 tcatctgtaa cccctttaat ggctattaac tttccggttt tttcagtaat attgatggtt
    27481 ttaacccctt tacctcccct gttggtcact ctatagtcgg aaatatctga tcgttttcca
    27541 aatccttttt ctgatactac cataatagtc aattgtggcg aatcaacaca gaccattcca
    27601 actacttcgt cattttcatc ggcaagggta acacctttta ctccggaagc attgcgtccc
    27661 acaggcctaa cattttcttc gttgaaacga attgctttcc ctgatttaac agcaagcaac
    27721 aattcgtttt ggccattggt gagttttgcc tcaatcaggc agtcgccttc gcgaatggta
    27781 attgcattga tacctgtttg acgtggacga gaataagctt caagagatgt tttttttata
    27841 ataccttttt tggtacaaag aataacattg tttgacttga taaaatcttg atcttccaat
    27901 gatttaacat taataaaagc cctgatttta tcatctggat ctatgctcag gatattttgt
    27961 atagccctgc ctttagaagt acggcttcct tcagggatgt cgtaaacttt aagccagaaa
    28021 caacggcctt tttcggtaaa gaacaatatg gtattatgca tagtggcatt aaacatatgt
    28081 tctaaaaagt cttcatctct ggtattacta gcttttgaac ctattcctcc tctgctttgg
    28141 gtcttaaatt cacttaaagg agtacgtttg atatatccaa gatgagaaat ggtaataatc
    28201 atatcatcat tggcataaaa atcttcagga ttaaactcgg tttcgtctaa ccttatttcg
    28261 gtacggcgct catcagcata tttttcgcgt acttccataa gctcatcctt gataattttc
    28321 attcttagcg attcatcaga tagtacagaa tttaaatatt caatcaattt gaccaaagca
    28381 tcatagtcgt ctttgatttt ctgtctttgc aacccggtaa ggctttgcag gcgcatatcg
    28441 aggatagccc tggcctgtaa ttctgaaagg ttgaaatttt ccatcagccc gtttcgagct
    28501 tcttcaggag ttttagactc acgaataagc ttaattacct catccagatg gtccagggca
    28561 ataagcaagc cttctaaaat atgctttttc ttttctgctt cttctaaatc aaattttgcc
    28621 cttctaataa caatctcatg cctgtgttta acaaagtaat gaataaggtc ttttaacgac
    28681 agcattttgg ggcggccgtt gactaatgca atattattgg tattaaatga attttgtaac
    28741 tgggtatatt taaaaagctt atttaaaata acattagcgc tggcttcttt tttacaaatc
    28801 ataacaatgc gcatgccctc acggtcgctt tcatcgttaa ggtatgaaat cccttctatt
    28861 tttttctcgt tgataaggtc ggcgattttt tggattaatt ccaatttgtt caccatgtaa
    28921 gggatttcgg taacgataat cttttcacgg ccagtctcgg tagtttcaat ttcagtcctt
    28981 gatctgacta atactcttcc cctacccgtt tcataagctt ctttgatacc cgaagtgcca
    29041 taaataatgc ccccggttgg aaagtcaggg ccttttatat attgcataag ttcttcggtg
    29101 gtaatgccat tattatcaat gtaagcaatg gtagcatcta tcacttcgcc caagttatga
    29161 gggggcatat tggtagccat acctacggca atacccgaag ccccatttat caaaaggttg
    29221 gggatacggt taggcattac agttggttct tcaagtgtat catcaaagtt taggcgaaaa
    29281 tcgactgtgt ttttttcaat gtccgccatc atttcttcgg ctattttcat taagcgggct
    29341 tctgtataac gcattgctgc cgggctgtcg ccatcaatag agccaaagtt gccttgtccg
    29401 tcaaccaagg ggtaacgcaa tgaccagggc tgagccatac gtaccatagc ttcatatact
    29461 gataagtctc catgagggtg gtacttaccc aacacttctc cgacaattct tgctgatttt
    29521 ttataagccg aatcagaagt caccccaagc tcggacatac cgtacaatac cctgcggtga
    29581 acgggtttca ggccatccct tacatcaggc agggcacggg ctacaataac cgacatagaa
    29641 taatctatgt aggctgtttt catttcctct tcgatgttta tttttattat gcgctgatct
    29701 tcagacatat ataattatat tttcgtaaaa aattaaagca cgaaaatagg tatttaaaat
    29761 ggaaattcat attaattgat gttgataatt attaacagca ggtgtttaat aatatatgtt
    29821 taaatataga tatatttgca tatatttaat tgaaatttgc cctaaatatc aaacgtgtat
    29881 ggatgccaat ttttcgcaaa agataaaaga agttttatca tatagtaaag ccgaagctgt
    29941 caggttgcac aataattata tagggccaga acatttcttt ttaggaatat tgaaagatgg
    30001 agaaggtctg gccgttgaag tattagcaga acagcttgat ttggaagata taaaaaacaa
    30061 actggaactt cagttggtta gcaacagcga attaaatacc attgaaactg aagatatacc
    30121 tatgcttaaa tcagctgaaa gggttttaaa acttgtttac ctcgaagcta aacatttaaa
    30181 gcaggaaact gttaatacag gacatttatt attagccatt cttcgacagg aagataattt
    30241 tgttactgat ttattaaaag agaaaaacgt gacttatcaa aagataaaag aagaattaag
    30301 caaaagacag cccagggcca gctctgacta tggcgatgaa gacgatgacc gttcagggcc
    30361 atttgggcaa caaggcaagg gttcacccgg aaatacgcct cagcagtcaa aatcaaattc
    30421 agacacccct gtgctggaca attttggcat tgacctgacc aaggctgctg aagaaagccg
    30481 ccttgaccca attgttggcc gtgaaaggga gattgaacgt ttagcgcaaa ttctcagtcg
    30541 tcgaaaaaag aacaacccca tccttattgg agaacctggt gtaggaaaat ctgctattgc
    30601 agaaggattg gcattgcgca ttgttcaacg aaaagtatca agggttttat ttggcaagcg
    30661 cgtcatagct ttagatttgg catctatcgt tgccggtacc aaatatcgcg ggcagtttga
    30721 agagcgtatg aaggctattt tgaatgagct tcagaaaaca cctaatataa tattgtttat
    30781 tgatgaaatt cataccattg tcggggctgg tggagctacc ggttcgctgg atgctgcaaa
    30841 catgctcaag ccagctttag cccgtggcga aattcaatgc attggggcta caactttaga
    30901 cgaatatagg caacacattg aaaaagacgg ggcacttgag cgtcgttttc agaaagtgat
    30961 ggtagaacct acttcgccag aagaaacact tgaaatattg aacaatataa aagagaggta
    31021 tgaagaacac cacaatgtaa tttatactgc agatgcatta gaggcttgtg taaagttgac
    31081 atcaagatat atcacagaca ggcacttgcc tgacaaagct atagatgcct tagatgaagc
    31141 cggttcaagg gttcatattt caaatattaa tgttcccgaa cgaattatca atcttgaaaa
    31201 agagattgaa gaaacaaaaa atgaaaaaat caaatcggtc aagaaccaga attttgaacg
    31261 tgcagccagt ttcagggaca aagaaaaaga actgcttgaa aaattagaaa tagaaaaaga
    31321 ccgttgggaa aaagaattag cccagaacag ggtcatagtg gattcagaaa aagtagctga
    31381 agtagtggcc atgatgactg gcgtgcctgt tcagcgcatt gcccaagccg aaggtacccg
    31441 actgctcaaa atgtcagaag agttaaaagg cagtgttatt ggacaagatg aggccatcga
    31501 gaaagtggtc aggtcaatcc aacgtaaccg tgccgggctt aaagacccta ataagcctat
    31561 cgggacattt atcttccttg gccctacagg tgttgggaaa acccaattag caaaagtatt
    31621 ggccaaatat ttatttgaaa gctacgataa cctgattcgt atcgatatga gcgaatacat
    31681 ggaaaaattt tctgtatctc gccttgttgg ggcgcctccg ggctatgtag gatatgaaga
    31741 aggcggccag cttactgaaa aagtcagaag aaagccctat tctgtggtac ttcttgatga
    31801 aatagaaaaa gcccacccgg acgtttttca tttattgttg caggttcttg atgaaggcat
    31861 cctcacagac agtttaggaa gaagagttga cttcaaaaat accattgtta ttatgacatc
    31921 aaacattggt acacgtcaat tgaaagattt tgggcaaggt gttggttttt cgacctcggc
    31981 aaaaatagcc caggcaaacg attatgccaa gagtgttatc caaaatgcct taaaaaaatc
    32041 ttttgcccct gaatttttaa atcgaataga cgatgttatt cttttcaata cattatctaa
    32101 agagcatatt tctaagatta ttgatattga gcttaaaggg ttatatgacc gggttatatc
    32161 tatgggttac cgtattgaat taacctctga ggctaaagat tttgttatag aaagaggcta
    32221 cgatgttcag tttggtgcca ggcctctaaa acgtgctatt caaaagtatc ttgaagaccc
    32281 aatggcggaa gctattatca aggcaagtat ccacgaaggc gataccctta ctatagattt
    32341 tgatagtgaa aaccaggaga tcaatatcaa aatcaaacca ttggaaataa aagacaacgt
    32401 tgcagagaac taaaaattgt ttgaaaacct tccaggtttc aaaaacctga aaggttttca
    32461 actgttttgg cccattttca aaaatggtta atctttttat aaataaatca ttgttgtccg
    32521 gtacaaacag ctcgccgcta tgcggctcag aaacaattgg tgtattgggt tatcctacaa
    32581 acagttcgca gctacgctgc tattatttag tcgcgtagcg acgttttgtt tgtagtaaaa
    32641 ttaaacatcg ttaaaattaa gccccagcgg ggcgacctgt ttctaatcaa cgatattaca
    32701 agtatttatt ttaccggaca acagggtaaa taaatatttc tctaaaaata atttaagtac
    32761 agaaagtatt atacaacata cgtttatacc attgtttttc aaacatatct gcaagaactt
    32821 agtgtaattg tgcaataaat tgattatatt tataacacaa attatactat cattggagaa
    32881 aaatttggac caatgacaaa caagtttagt atattgctta tagtgctggt ggctttgggg
    32941 tgttcaaaga aaaataccct tgaggagaaa agtttgcaag ccaatactaa ctctatttcc
    33001 aacaacttgc aaaaacaagt ccgtatcatg gggcactggt atggagaagg gaaaaaagaa
    33061 ctactggtga gggaaatggt gcgagaattt tctttgctta accaggattt gagcattgaa
    33121 ttgttgtttc cacaacaatt tttgaaatac aaagaagaat cagagttata ttttgtagag
    33181 tgcgatacca tagaacagat ggtaaggaaa aattcctggc catttgaagt gttgttttgt
    33241 gaccaggaac gatataaaaa gattggacaa cagctcaatg accccaattg gggcgaaaaa
    33301 taccttgtgg atttttccaa tgaaccttgg tttaagcatg cccataagga tggtttgatt
    33361 gaaacaatga acctcaaggc aaggtatggc aatattatgc cgggcattgt ccttgaaggt
    33421 attaccaata ttttgtttgt cagtgaatat gtcgaaaata agcttgggat aaaagtcaaa
    33481 gacctcgaca tgacatttga cgattttata gcttatgccc aggctgttga caattataac
    33541 aaaacccata gcgataaaat cagttttctt tctcaacaat acaaaacagt taatgatata
    33601 ttatttaccc agctggccac ctctatttat gaaggagaca aatacgaatc gagagaaaaa
    33661 ggtatagcag ctttaaaaaa agcttatgca gaatttgaaa agctttcgaa atataacccg
    33721 acaaagcaat acatggatta ttcgggatat acttatgatg aagcccaacg catattatat
    33781 gataaacagt gcctgtttac catgaacccg acctggatga tattgttatg gcttaagtct
    33841 aatccagaag ggacaaaaca attacgtcca tgcgaaatac catcttttgc cggcaaaaga
    33901 tcgccttttt attcaggttt ttaccaggta atatttgtgg ttgctaaaaa ttctaaaaat
    33961 cctgaagccg gcaagcgttt tatgaaatat atggccagta ccgaaattgc cgacaaatgg
    34021 gtgaaatatt cgagttgccc taccgggatg cgaacctcta tttcatatac cgattttgga
    34081 caaaacaagt atgaaaagtt ttttcagcac atccaaaaaa aatatggtaa caaccaggca
    34141 gaagtcagtt tgcctaagtt atttctaaac aataatacaa cagcatctat tgattatgga
    34201 ttaaatgagc ttttgtccgg aaaaatcaaa gctgatgaag cttatcaaag aactatgaga
    34261 caaattaagt aacacacacc aactaataaa atacatcact atgaattcct ctataaataa
    34321 taaagtcagg gaaaaactta gcctcaagat tggattacta atcattgcaa ccgtcatatt
    34381 agtattactt tcttcgggag ctttttatat ttcaaagttt agctctgata ccaacaaacg
    34441 tttttataaa caattggcag cccctgccct attgatgtct tcagggaaac ttaagtatga
    34501 tgccgccatg gacctgaaaa cgatatccag cttagttggt gattcggtaa tatactcatt
    34561 ggttatcggg gtcaatcaaa agatttatta ttcaaacgac agcactattt tggacaaaaa
    34621 agtagatgaa gtaaaatttc ttagccctta tgatgttttc aaagcaccta taaaagagcc
    34681 atattatgct gaagccaata atgggaatga agtgatatgc atttcgccat tatactttga
    34741 ggatggcaaa tatttgggct acctttttat agccacagat accgcagcga tgaacaaatc
    34801 aaaaaatagc ctgattttga tattcttgtt tggttcgtta atcacagtgg ccattctttc
    34861 tatactgatc atgatattgt ttcatcgtta tataaccatc aggataaaga caatacttca
    34921 ttctctgacc attattaaaa atggcgattt gtctaaaaag attgaattaa acacccaaga
    34981 tgaactgggg caaattgccc aatcggtcaa tgagctgacc ttgcagttta aaaccatcat
    35041 ccaggaaatt attggtgaaa ttgaaaggtt gcgcagttcg agcgtagagc taaacagcag
    35101 tgcccaaagt atgtctgaag atgccaacaa cctggcttct attgctgaac aggtggcatc
    35161 atcgatggaa gaaatggttt ccaatatcca cctcaattca agcaatgcca tgactaccga
    35221 gaacatttca aagcaggcag ccaaagaaat ggacgtggta ggcgcttact cgcaggaaag
    35281 cttgaaattt attaaagaaa tagcccaaaa gatttctatc atcaatgata ttgcattcca
    35341 aactaatttg ttagccctaa acgctgctgt agaagcggca cgtgcaggtg aggctggtaa
    35401 aggcttttcg gtggtagctg ctgaagtgaa aaaactggca gaaaggagcc gtgccgcagc
    35461 tgatgaaatt cataagctat cttctgtttg cgttgcgcag accgaaaaat ctgtttcgag
    35521 tgtaaaaatg cttgaacccg aaattatgaa gacggtccaa ctggtacagg aaatatcttc
    35581 agctactaac gagcaaaatt cgggtgccga ccaggtaaac aatgccttac aacaactcaa
    35641 tgttattaca caaaagaact caagcaattc tgaaaatatt gaagaccagg cttcagccct
    35701 ttcacaacag gccaataagt tgaacgagat agtatcttat tttcagatag aagaaaagta
    35761 gcctattctt tcagttcagt ttgtataata cgaattacct ccaaaagggg taattttttt
    35821 tgtatggcaa tgagcttgca atcctcgtat tcgggcttaa attttacaat gttgttccag
    35881 aaagacttct ttaccctgac ttttcccaaa gaggttgaaa aagtgagtat ttccctttgc
    35941 aagatatgct tggtaaccgg gtaagtgcgg actcccagcg ttgtggtatt ttctaataca
    36001 atattaatca aggtattttc taaactcacc gggctaagta ccgataacac aaaagccggc
    36061 ctgttttttt tcatgtttac aggcgtaaca aacacctcct gggccccggc agcataaagt
    36121 ttttcaaaaa caaaatcaaa cacttccgaa gacatatcgt caatattgca ttcgagcatc
    36181 atggcttgtt cggttggaaa gtcttttgct tctgtttcag cagcaataac ccgcagcaca
    36241 ttgggtttgg catcttcttt gtgccccagg ccataggcta ttttcgaaat agaaaaatta
    36301 gttaggtctg taaactcttc caccagctcg gccaatatag cagcgccagt cggagtagtt
    36361 gattcaaaag gcatcccgcc agttttaact ggcaaacctt tcaatatttc tatagttgca
    36421 ggtgccggga ccggaaaaac gccatgctca catttgacca tgccactacc aagctgaata
    36481 gcagcagaag ccaaaatttt tgaaggcttt aaaatatcta cacaaatagc agcacccaca
    36541 atgtccacaa tagaatcgac tgctccaacc tcatgaaaat ggacattatc cacctcgcag
    36601 ttgtgtactt tagcttcggc cacagccaca cgatgaaaaa tacccaggct tatcttttta
    36661 atattgtcat tcaagctcga atcttctatc aattttctga tatccttata acttctgtga
    36721 acctgtttgt gcttatgttg gctcgcgcct tttgttgcag acagcggaat atttaactga
    36781 aaagcaggtt tgggcgatga ttcaagcaat acatccacca aagtgccaga aataccatta
    36841 cgcattgact ttgacacctt aatctggtaa ccactgagtt tcaatttctt caattccgaa
    36901 atcaatacat cagcgtcaac cccaaggtcg atcatggctg caaggttcat atccccgcta
    36961 attcctgcaa aacaatcgta atataaaacc ataaactaat tttttacgaa ggtaactaaa
    37021 tatatacttt aaaataaata tcacttaaca ttccttaaca gcaaattaac aaagaaagag
    37081 cgtcttttaa gaaacaattc catcaaatgg atttagtttt gtctattcga aaaaatggcc
    37141 taattttaag aaagaacaat taaataccat tactatgaaa agacgcgaat ttattggcaa
    37201 aagtttggga gcaactttgt tggccggtgc cgggttaagt cttggcacaa cccccgattt
    37261 atttgcatca gaaagtagct catcaaaagc cggatcttat gatttggttg ctgtaagagg
    37321 cggtgaacct gaagtaatgc ttgacaaagc acttgaagca ttaggcggaa tgcagaaatt
    37381 tgttaaaaaa gggcaaaaag tagttgtaaa acctaacatt ggttgggaca aaagccctga
    37441 atttgctgca acgacaaacc ctgccttagt aaaaagaatc atagagcatg tatataaagc
    37501 cggggcaaaa gaagtgattg tatttgactt cacctgcgat aaccccaaaa agtgttacga
    37561 aaactcaggt ataaaaaagg ccgtagaaga agctggcgga gttttggttt ccggtaataa
    37621 tgaagcggat tacaaaccgg tttctgtccc taaaggaata aaactgaaag aagccaaaga
    37681 acacaagcaa atactggatg ccgatgtggt tatcaatgtt ccggtactca aaagccacca
    37741 gggttcaact gttaccataa gtatgaaaaa cctgatgggt attatctggg acagaggata
    37801 ctggcaccga aacgacctgc accaatgtat tgccgacttt gcaacctatc gtaagcctac
    37861 tctgaacatt gttgatgctt accgcgtgat gatgaaaaat gggcccaagg gtgtatcggt
    37921 ttcagacgtt aaattgatga aatcgttgat tgtatctaca gatattgttg ctgccgatgc
    37981 tgctgctacc aagttgtttg gtttagagcc tcgcgatatt gaccatatcc gtattgccca
    38041 cgaaatgggt gttggcaaca tgaaccttga caaacttaat attgccaggc taacggttta
    38101 attaatattc tataatgcag tatttacgta caattcgtat tgtcacttcg gtaataatat
    38161 ttgccgttgc tttcatttct tttgtcggac ttccggcttt gttgttgccg ttattcatcc
    38221 aggggccggt gttaaaaatg caattggtac cttcaatact gaatttttcg gtagtgagca
    38281 tggccagctt tgcttttatt atcattatcc tgctgacgtt gttgtttgga cgtttttatt
    38341 gctcaagcat ctgccctatg ggtactttgc aagatatcat gaacaggttg agcaagccat
    38401 ttaggaaaag aaagattttc aggtttacct taccttataa aaaaaccagg tatatcattc
    38461 ttgcccttac cattgttcct ctttttctaa gcagttcaat aacgttagcg ctgcttgacc
    38521 cgtatagcaa ctttggcaaa atcatttcgg cctttttcag gccaattact tatggtttaa
    38581 acaacctgat aagcaacatc cttgcccact ttgatattta tagcgttgac ccggttatta
    38641 tgggcagcat ttctgttact gccattattt atgcttcggt tcttcttata tcattggttt
    38701 tgctggtatt ctttcacgga aggctttatt gcaacagcgt ttgcccggta ggaacaacct
    38761 taggtttact ttccaagttt tcattttttc aacttaaaat tgaaaacact aaatgcaccc
    38821 aatgcggcaa atgcagcgct aattgtaaag cagaatgtat cgatattaag cacaaagagt
    38881 tagactttga aagatgcatt ggttgttaca attgtattaa cgtatgtacc gaaaaagcaa
    38941 ttagttacag caagttaagt ccagtaaagg ttgaaaaaga aacttcaact gatgaagcta
    39001 aacgttctgc attcaaaacc cttgcagcca caggattggg attagttttt gccaataaat
    39061 atgccttttc gcaacatgaa acaggaaaga aaaacaccat tgaagtaaaa aagaaacata
    39121 ccgtttcacc tcccggttca gaatcgcata agcatttttt ggcgcactgt accgcttgtc
    39181 atctttgtgt aagccagtgc ccttccagcg tgctgaaacc gtcttttctg caatatggat
    39241 tagaaggaat gatgatgcct tatctggact tcgaaaaaag tttttgcaac tttgaatgta
    39301 cccaatgtag ccaggtttgt ccgactgggg ccataaaacc cattgacagc aagaaaaaag
    39361 ttactgtgca aatgggaata gtccaattta taaaagaaaa ttgtatagta tatcttgaag
    39421 aaactgcctg tggggcatgt tcagaacact gccctaccaa agctgtgttt atggttcctt
    39481 acaaaggcat gataactatc cctgagacca ataaagacat ttgcattggt tgcggggcat
    39541 gtgaatttgc ctgtccggca aagcctaaca aagccattta tattgaagga aataccttac
    39601 atttgacagc caagaagcct aaagaagaaa agcaggaaga gtcaaacatg gaagaatttc
    39661 ctttttaaat tttaaactat tttatgaaca aagaatatat tgataaaata ctttcccagc
    39721 ttaaaaatgg agaaattgaa gtaagtcagg cttcagagtt gttgaaaaca ttgccttatg
    39781 cagaacttgg ttatgcaaag gttgaccatc accggacttt acgaagggga tatcctgaag
    39841 ttatctttgg tgcagggaaa acacccgaac atatcaaggg aattgtagca aatatgctca
    39901 ataccaccga tagcaatata ctggtaaccc gatgttcaaa agaagccagt gaacaagtgt
    39961 taagtatttg tcctactgct gaatatcatc cgttatccaa agcattagtt ataaaacaaa
    40021 aagagataac taaaaccgaa tcctatattg ctgttgttac tgccggaacc agcgatattc
    40081 ctattgcaga agaagcacaa ataactgctg aaatatttgg caacaaagtc gagactatat
    40141 ttgacgttgg cgtagccggt attcacaggt tgtacgacca agttgacaaa ttgcggggag
    40201 ctaaagtact catagttatt gccggcatgg aaggcgcttt gccaagtgtg gtaggcggtt
    40261 tggtggacaa acctatcatt gccgttccta ccagtgttgg atatggagct gcttttggag
    40321 gtattgcagc ccttttaggc atgctcacca gttgtaccag cggcatttcg gtagtaaata
    40381 ttgacaatgg gtttggagct gcttatcagg caagcttgat caatcagtta taacataacg
    40441 tcagttcgac aaaagaaaaa tgcttacgta tttgatattt actggtattc ctaacttttg
    40501 ccttgaagca aaagttacaa aaattcaagg cttgccgtaa a
//
LOCUS       DAOSPF010000030        39774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1050_length_39774_cov_6.046401, whole genome shotgun sequence.
ACCESSION   DAOSPF010000030 DAOSPF010000000
VERSION     DAOSPF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1050_length_39774_cov_6.046401"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1005)
                     /locus_tag="PK252_07640"
     CDS             complement(109..1005)
                     /locus_tag="PK252_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14619.1"
                     /translation="MKKFIIYIILFTCLILVSIYLILLKADGYSDPYYLKFTTPRQNS
                     LILGTSKAAQGIIPDVANKILGNDIYNYSFNMNISPFGPKYLESIKRKLSQKSHSGIF
                     IVTVDCWSISCMGENPNDTTKFKENNSFIGQIKIVDQKPNLHYLLKYMFGNYYRIIFK
                     SSDAVLHENGWFEVSINTDSVSVNRRTKSTLQEYKNFLSNYKYSQARFDYLVKTIEFL
                     NKFGSVYLVRLPVSKGLMDIEHQCMPNFDYKIKQIADKTCGYLDLTPKNETFSYTDGV
                     HLDKRSGKIVTEEIAQWIKNEK"
     gene            complement(1011..2456)
                     /locus_tag="PK252_07645"
     CDS             complement(1011..2456)
                     /locus_tag="PK252_07645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOT14620.1"
                     /translation="MLFNSIHFAIFLPIVFTLYWFVTNKNLNLQNILLLISSYFFYAC
                     WDWRFLFLLVFSTSLDYFTGIKIYDATTKRNKLFWLWLSICINLGFLGVFKYYNFFAE
                     SFTQGLHLLGINANLGSIKVILPVGISFYTFHGLSYVLDLYKNKIKPERNFVNYSVFV
                     SFFPLLVAGPIERATHLLPQIVKKREFNYYTAVDGLRQILWGLFKKMVIADNCAIYAN
                     MIFNNQADYSGSTLVLGALFFTFQIYCDFSGYSDIAIGTARLLGIELLRNFAFPYFSR
                     DIAEFWRRWHISLSSWFRDYLYIPLGGSRGGTWMKVRNTFIIFIVSGFWHGANWTFIV
                     WGALNALYFLPLLLTDRNRIHLQTVAQGRLIPNLKELLAMIVTFSLTVLAWIFFRSQN
                     LEHAINYISGIFSSNLFSMPYFLGIKESAITVGFIIFLLIVEWIGREQQYAIKIASHW
                     DKPLQWLFYYLIAMIIFLFGSPSQEFIYFQF"
     gene            complement(2923..4422)
                     /locus_tag="PK252_07650"
     CDS             complement(2923..4422)
                     /locus_tag="PK252_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOT14621.1"
                     /translation="MITPIQKKQLEEMGISEEQLNQQIQSFIKGFEFAALVRPATIND
                     GIIVPSEKEIEKWTKLYDEYSKTASILKFVPASGAASRMFKNLYEFLDNPSANVGGSI
                     MMRSIDSFAFYQDLSGALKKLGYDIRTMHNVDDTKIVVETLLTNKGLNYGSLPKGLLK
                     FHAYNNETRTATEEHFVEGLMYAKGANKLQLHFTISVEHEQLFIAEFEKLKAKYDKEA
                     ELSISYSFQKKSTDTVAVDVNNVPITDKDGKFVFRPGGHGALLENLNDIDADLIFIKN
                     IDNVVPDHLKHVTVKYKKALAGLLVFVKTEINEYINYLEANTTISETLKERIATFMLK
                     YMGITLPKGMDNSVFAAYVKSKLDKPIRVCGMVRNVGEPGGGPFWVLNKKGEANLQIV
                     ESSQVDMNNHQQKVIFDGATHFNPVDLVCSTKDYSGFKFNLLRFRDNNAGFISEKSVS
                     GKKVKALELPGLWNGGMAHWITLMVEVPILTFNPVKTYADLLRKEHQPQ"
     gene            complement(4459..5043)
                     /locus_tag="PK252_07655"
     CDS             complement(4459..5043)
                     /locus_tag="PK252_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT14622.1"
                     /translation="MKTDIILAGVGGQGILTIAASIGYAALHDNLNLKQSEVHGMSQR
                     GGAVQSHMRLSSNPIASDLIPLGSADVILSVEPMESLRYLPYLSKNGWIITNSLPFIN
                     IPNYPEYQKILDEINKIPNHVLIDADKIAEEAGSVKSSNIVMLGAASPYIEMSYESFI
                     EGIKKLFGRKSEEMVNLNIKAFDLGRKISMEKKK"
     gene            complement(5062..6654)
                     /locus_tag="PK252_07660"
     CDS             complement(5062..6654)
                     /locus_tag="PK252_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOT14623.1"
                     /translation="MDKILLLADEAIAQAAIDAGLSGVYAYPGTPSTEITEYIQASEE
                     GKSGKIRSQWAANEKTAYEAALGMSYAGKRALVCMKHVGLNVAADGFINSAITGTNGG
                     LVIVVADDPSMHSSQNEQDSRFYGKFAMIPMFEPADQQEAYDIVYQAFDLSEYFKLPV
                     MVRIVTRLAHSRAGIVRRAAKAQKNTQIPDDSKRFVLLPAIARQRYKSLLGQQKEMME
                     IAYNSPFNKFTNHSNKSIGIIACGITYNYLLENFPDGNIPYPVLKLSHYPISQQLISQ
                     VYDECETVLVLEDGYPFVEEQLRGIMGVGTRIHGRMNGILPRDGELTPDIVGAALGLK
                     QNIKVTEIPEVVTNRPPSFCKGCAHIDIYTALNEALKDYTPGRVFADIGCYTLGALPP
                     FNAINSCVDMGASITMAKGAYEAGLQPAVAVIGDSTFTHSGMTGLLDAVNDNVGITVI
                     IADNATTGMTGGQKSSATGRIEQICEGLGVEKEHIVVFNPLKKNHEEAVALLKKELEY
                     KGVSVIIGRRECIQTLSRKHRN"
     gene            7340..8827
                     /locus_tag="PK252_07665"
     CDS             7340..8827
                     /locus_tag="PK252_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14624.1"
                     /translation="MKKKFIQFAKWSLCISTLVILSVIWYYNNGSNGPIPLILVAAFS
                     LLMYMFVGWQRWIVLTLYILVLAVHFLIEWYFPGRIKPYPDEKTRLIDFYSSAAMYVM
                     LMSLIMIYIKNINQQQKEKADRANELKTKFLSNMSHEIRTPMNGIIGFAQMLKKTGLT
                     EEKRERYVNAILTSSKQLLNIVNDILDISKIESGMISLSLRPVNIKKVIFNVIANNEL
                     LAVQKGLQLLYNIENYKENELFLADEQKLGQVLNNLVNNALKFTSNGSITIHCWVLEK
                     ELKFCVEDTGSGIEEKYQGAIFDRFQQVMNGNKENVGGTGLGLAICKNFVTIMGGKIW
                     LESVPEMGSKFFFTIEKKIPEESSTTKNNEKDIKRVDNLKFKSTSILVVEDDVNNYNL
                     LKEILAERGVTTFWAQNGKAAIKMVEENPHIKLVLMDIKLPDMTGVQALFQVLKIRPL
                     LKVIATTAYAFEEEKKELMSVGFADYVSKPIKVELLFKALNDSLS"
     gene            8966..9523
                     /locus_tag="PK252_07670"
     CDS             8966..9523
                     /locus_tag="PK252_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14625.1"
                     /translation="MKRNIFIGLFILFSVGLLSQSKTAYTVGVNSLGNYGLKVDRGDS
                     LSFFSFSYTSFNFNNTFNSKGFSMNAPSLGNSNMFDVAYFRNKDLSKRLTFVYGGGIS
                     LVSFPNITSSSVVVKNGGYKPNFDLNLGLVYKISDKVSVSASFNQRYGYSPYFYDNSL
                     FGNSFLSPFHNAGFNSFHNSPFDPF"
     gene            9866..11914
                     /gene="htpG"
                     /locus_tag="PK252_07675"
     CDS             9866..11914
                     /gene="htpG"
                     /locus_tag="PK252_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOT14626.1"
                     /translation="MSKGKIGVSTENIFPIIKKFLYSDHEIFLREIIANAMDASNKLK
                     ALASTGEYKGDVSNLKVSVAVDEKKKTITISDNGIGMTADEVEKYINQIAFSSAEEFV
                     EKYKNQANSIIGHFGLGFYSAFMVSKKVELFTRSYKEGSKAVRWECDGSPEYIIEDTE
                     KETIGTDVVLYLDDESADFATQSKVNELLKKYCKFLPVPIAFGKKKEWKDGKEVELAE
                     DNIINNPTPAWTRKPSDLTHDDYMKFYRELYPMAEEPMFYIHLNVDYPFNLTGILYFP
                     KIKNNIEISRNKIQLYCRQVFVTDSVEGIVPDFLTLLHGVIDSPDIPLNVSRSYLQSD
                     QNVKKISSHITKKVADRLQELFNNQREDFEKKWDNLKLFINYGIITDEKFWERAEKFT
                     LLKNVSDKYFTFEEYQKIIKENQTDKHGSLVYLYASDKEGQHSFIEAAQNKGYDVLVM
                     DGQLDTHFINHVESKLKNSRFVRVDSDIADNLIQKEEAKEVKLTQQQKDMLSPVFKSV
                     MPSNGQYYVSFEAMGENDMPAVITRNEWMRRMKDMSALGGGMNFYGEMPDNYNLIVNA
                     NHPLVNKVLQQTEEAKQAEIKETVEQITKLTAEREALMALHKDKKYDEIPQAERDKEA
                     ELDKKIDELEDKRNKVLEEYGKTNPLVSQIADLALLANNMLKGEALSRFVKRSIELIK
                     "
     gene            complement(12018..13319)
                     /locus_tag="PK252_07680"
     CDS             complement(12018..13319)
                     /locus_tag="PK252_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOT14627.1"
                     /translation="MAQENWKFETLQLHAGQEVDPTTNSRAVPIYQTTSYVFNNSEHA
                     AKLFGLKEFGNIYTRIMNPTTDVFEKRIAALEGGVAAVAVSSGQAAQFLALTNILQAG
                     DNFVSTSYLYGGTYNQFKVQFKRLGITAKFVDGDEPAEFEKLIDKNTKAIYIETIGNP
                     RFNIPDFDAIAAIAQKHGIPLVVDNTFGAGGYLFRPIEHGANVVVESATKWIGGHGTS
                     IGGVIVDAGNFNWGNGKFPQFTEPSEGYHGLKFWEVFGSAGPFGNIAFAIRARVEGLR
                     DYGSSLSPFNAFLLLQGLETLSLRVQRTVDNALEIAKWLEKHPKVEFVNYPGLESDPY
                     HKLAKKYMKHGFGGVLSFKVKAGKEAADKVVNNVKLISHLANIGDAKTLIIHPATTTH
                     EQLSPEEQKKSGVIPGQLRLSVGIEHIDDIKADLEQALAIA"
     gene            complement(13421..14080)
                     /locus_tag="PK252_07685"
     CDS             complement(13421..14080)
                     /locus_tag="PK252_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14628.1"
                     /translation="MNNKIQVIIADSQQLIVEGLVSIIYNEDKFEVKGIAEDIAYVHQ
                     LICPEDKGVVITDHQMAGIDNIEDLSRLKSKCPDIKILILTNSISRDEINVLNSIGIS
                     NILLKTTDKDDFLQALEATYKGKKYYSSELMETILGESKSKGKQKVKANLTKAEIEIV
                     RQIAEGLTTKEIADKKNISFHTVMTHRKNIFRKLEINSSSELIMYAMKNGLIDSIEYY
                     I"
     gene            complement(14149..14835)
                     /locus_tag="PK252_07690"
     CDS             complement(14149..14835)
                     /locus_tag="PK252_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT14629.1"
                     /translation="MEEIKTKTVLESLPGLNCGACGYRTCNDFAKAIDSKESEIKKCI
                     HLKPMASIETGRFNCINCVHTENLGKKLDWKDSLQRDFDFILDIIEGEPGPKETILPY
                     NPSLVKDLGVKKGDVMVGRPMGMSCGCPVTHCGVVYDVDPRNGVIYWHVTGPLRPRSE
                     GFVDIGFYVSQAYEGIIKESKEKIELGRRYWFLPRRCMLQWRHSGLVNAVTKLKDGSY
                     KVRIEGLLIG"
     gene            complement(14847..16010)
                     /locus_tag="PK252_07695"
     CDS             complement(14847..16010)
                     /locus_tag="PK252_07695"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT14630.1"
                     /translation="MKGIYLDNAATTPTDPRVIEAMMPYFTQIYGNASSMHTYGTRSK
                     EALENSRAKIASYIGCQPDELLFTSSGSESNNMALKGIAFANRNKGNHIIVSAIEHDC
                     ILNTCKWLEEEGFSITLVPVDSYGTLDLQALERAIKPETILVSVMYANNEIGTIQPIA
                     EIGAICRKHNILFHSDACQSFGKLPIDVVKSNIDLLTINAHKIYGPKGIGALYVRKNT
                     RITPILHGGGQENGLRSSTENIAVIVCFAKAAELAIKDMDNENIKLFTLQKSIIDDLS
                     ENMEGFYLNGHPNNRIPGHLSFSFQGLEGDTIRLLLMLDEMGIAVSAGSACSSNHGGD
                     GTSHVLKAIGLNQFEARGAIRVSMGRYTTDSDIEIFKKTIKQKVLQLNSIFSH"
     gene            complement(16071..16772)
                     /locus_tag="PK252_07700"
     CDS             complement(16071..16772)
                     /locus_tag="PK252_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT14631.1"
                     /translation="MKLIIFAGPPTCGKTTVIRQVIKRMIAQNQKTSYIKIDVLFADE
                     DETIREEFKIPTRKIYSGELCPDHCNVVVLDEAVEWAESTGAEYLFVETAGLCLRCSP
                     YIENSLGIVVLEATSGMNLPRKIGPMLTLADISVITKIDLISQAEREVFRYRVLEAAK
                     DIKVVESNALYGIGIDPIVRQITQAQDVEMPMYLRGNPPVGTCTICVGKKEIGAKNHF
                     GVLRTMEKELFYIGE"
     gene            complement(16777..17562)
                     /locus_tag="PK252_07705"
     CDS             complement(16777..17562)
                     /locus_tag="PK252_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT14632.1"
                     /translation="MDIKSIEIKGGQGKDGSKEQVDSLELKPGEILSIVGPTGCGKTT
                     LINDIELFANRNTPSGRQILINGDPIPAEFSFDPSKHPIALISQHTNFLSDLPVGEFL
                     TIHAKVRGAENIENVVNETIEFANQLTGEAIIKETAMTELSGGQTRSLLIADAVVIGN
                     SPIILLDEIENAGINKTKALELLKKYDKLFVFVTHDPIIALLSDKRIIMKSGAMQKVV
                     VANPEERLAVENLRKMDDIILHFRSIIRAGEELKAEHLNLIKL"
     gene            17673..18374
                     /locus_tag="PK252_07710"
     CDS             17673..18374
                     /locus_tag="PK252_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT14633.1"
                     /translation="MALNEQHRYFDCSQCLFKQLSCTLIEDSEFEVIRKTSYQMKYQK
                     GETILKQGAKSTGLLYLHSGIVKFSFKTEDQRNIITTIVRGPKLLGGANLFFKDINIF
                     SIVAVEDCDVCYIDSNALRSVALNNGRYTLAMFESTASMFQHSIFNFISLAHNHVNGR
                     IASILLYLWEHVYEGSGYDFIISRKEIAEFAACSHENVINTLSKFKKEGLIELNGKKI
                     EIVNIEALKELSKKG"
     gene            18376..18804
                     /locus_tag="PK252_07715"
     CDS             18376..18804
                     /locus_tag="PK252_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT14634.1"
                     /translation="MLSKKTRYAMMAMIKLATEYGKGPILIGDIAQSENIPQRFLENI
                     LLELKKMGYLGSRLGKSGGYYLLKHPKDVNLAEIIRHFEGTIALLHCVSEKAYQPCEF
                     CKNEAECKIKSVFKDIRDYTFRLLEGKTLEELASSKQTDM"
     gene            complement(18808..19401)
                     /locus_tag="PK252_07720"
     CDS             complement(18808..19401)
                     /locus_tag="PK252_07720"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT14635.1"
                     /translation="MNFDINNIPNDDPKRFIKIAEHFLQSLREHKISFAYEGEFTQQI
                     TIAFSALAESSISKADDKGLIQRRVNFVVVECMQNITKHAEIKSSNDPKPISRGIFML
                     SKSKEEYGITTGNIVERSRREALQKKIDYINNLSREEINELYMKQISEGELSEVGGAG
                     LGFIDIVRRTARKLNYHFLDIDENTSFFILTSFVSRN"
     gene            19608..21491
                     /locus_tag="PK252_07725"
     CDS             19608..21491
                     /locus_tag="PK252_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOT14636.1"
                     /translation="MQYWGFSQFRPLQEDIIASVLEKKDTLALMPTGGGKSITFQVPA
                     LAMDGICIVVTPLIALMKDQVEGLKKKGIKAVAVYSGMSKEEIDIAMDNCIYGDFKFL
                     YCSPERLGTEIFRMRLPKMNVNLLAIDESHCISQWGYDFRPSYLKIAEIREIIPDVPV
                     LAVTATATPIVVDDIMKQLKFRQPNVFRMSFERKNLVYLVREVEDKISYLLKVFGPTR
                     GSGIVYVRNRMKTKEISDFLNKNGISSDYYHAGLTDELRSSKQDAWKSGRVRVIVATN
                     AFGMGIDKPDVRTVVHVDLPDSPEAYFQEAGRAGRDGNRSFAVLLYNTNDKLSISQRI
                     ETNFPDLFEVKTVYNALGNFFQIPYGSGKYMAYDFDLAKFVGTYRMNVQKAYSSLKIL
                     EQEGYIEVTDSINNPSKVKFLLGRDDLYRFQVANEAFDAFIKLLLRSYEGLFSSYVSI
                     DESVLSKRANIPLDSVYKYLNKLSSAKILHYIPRRNKPVVVYTEERLEDKALLLSVES
                     YRLRKDRYVEKAETMLHYATATNKCRSVILLQYFGQNDAYRCGQCDVCLKRNEADVSK
                     YEFDLIVQDIKNLLHEPLLLVDISKKLEQRYGSEKLTKVIRYLLDNGKLKYNEEQYLQ
                     WVH"
     gene            complement(21498..22409)
                     /locus_tag="PK252_07730"
     CDS             complement(21498..22409)
                     /locus_tag="PK252_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14637.1"
                     /translation="MIKLLSNDQIDRAQWDKAIESSPYGLIYALSWYLDIVCPDWEAL
                     VSDNYEQVMPLPVKNKMGLLKYPILPTFTQQLGIFGQKIINSELTSEFVKNVKRQYNY
                     SNFSLNASNDISLLNDCCHNSGVTYILNLEKTITDIENAFSSNHVRNIKKAKEYKLNY
                     ASIPDIDFFLKFIDKHLPVKFGKKELKSAYHLYHEAFKRGMAEIVATSDEDKNILSMA
                     LWFNFNKRRTYILPATSSEGKEKRANFWLIYQYILLHANKDEVIDFEGSRIESLARFY
                     AGFGAEKTLFPHFHFNNLPFPLKMVKK"
     gene            complement(22406..23434)
                     /locus_tag="PK252_07735"
     CDS             complement(22406..23434)
                     /locus_tag="PK252_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14638.1"
                     /translation="MNKSFLCILALVGATIVAKAQQQTINITQMTFGADYNVYLKMDN
                     DSAYNYDFDKLIMADPDEVFNKKTEFIYYPVNFDQSYIDSLLNLNNQESDYTLNTASS
                     TAPRRATLWGSVGQTLGGGWAHFINCILFALESRQLEIADPILQRPESNWKPNPVTET
                     WKRTHKWRFYLPVEQKYAKEEYKARKKNNKLGDVNSIPQAYIKLMLHTSQREYNKLLK
                     KKDYKTLAKIDLVKLMLGSPYLGQQQIDYIKNRLITAISKYNRRNKPSVLIFDKYEAA
                     VAVSLDGTGYKAQKIVFRDENSLSPMQIMQRTTIIQGLIGLINVANNEAFKQKLNNLY
                     QTSPSEKN"
     gene            complement(23553..25511)
                     /gene="gyrB"
                     /locus_tag="PK252_07740"
     CDS             complement(23553..25511)
                     /gene="gyrB"
                     /locus_tag="PK252_07740"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT14639.1"
                     /translation="MQEDLDKETTSSYSADSIQILEGLEAVRKRPGMYIGDTGSRGLH
                     HLVYEVVDNSIDEALAGHCKNIEVFINEDNSITVADDGRGIPVDFHEKEQKSALEVVL
                     TILHAGGKFNKDSYKVSGGLHGVGVSCVNALSNYLKAEIHRDGKIYVQEYSKGKPLGP
                     VKTVGDTEKTGTIITFKPDETIFTITTEYNYEVLEARLRELAYLNKGIRLKLVDKREL
                     DENGQARQAIFYSETGLQEFVAFIDANREKLIEKSIYIDTEKNGIPVEIALHYNTSFT
                     ENVFSYVNNINTIEGGTHLAGFRRGLTRTLKAYAEKEGMLSKLKFDISGDDFREGLSA
                     VISIKVAEPQFEGQTKTKLGNNEVMGAVDAAVSTELANYLEENPRDAKQIVNKVILAA
                     TARHAARKAREMVQRKNVLSGSGLPGKLADCSDKDPATCEVFFVEGDSAGGTAKQGRN
                     RRFQAILPLRGKILNVEKAMQHKIFESEEIRNIFTALGVTIGTEDDSKALNLEKLRYH
                     KIIIMTDADVDGSHIATLIMTFFFRYMFDLIQKGHLYIASPPLYLVKKGKIEKYCWTE
                     EQRMAAIEEIGKGKGGVHVQRYKGLGEMNAEQLWSTTMNPENRILKQVTVENAAEADR
                     IFSMLMGEDVPPRREFIEKHAKYAKIDA"
     gene            25764..27191
                     /gene="hydG"
                     /locus_tag="PK252_07745"
     CDS             25764..27191
                     /gene="hydG"
                     /locus_tag="PK252_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOT14640.1"
                     /translation="MLYNPQNYKVADKPMEPFIDEQEIWDFIDSSKPDSHKVKDIIAK
                     SLDKHRLTLEETATLINCTEPGLVEQIKEGAKTLKERIYGKRIVLFAPLYVGNLCINN
                     CLYCGFRVSNTMQHRSTLTHDELVNQVIALEDKGQKRLILVFGEHPKYNPDFIYDAVK
                     TVYQVKKGNGEIRRVNINAAPLDIDGFRRVKEAGIGTYQIFQETYHRGAYAQYHKKGP
                     KQHYDWRLTALDRAQEAGIDDVGIGALVGLYDWRFETLGLIRHVNHFEAVYNVGPHTI
                     SFPRIQNASHLNIGKDYNVSNEDFTKMIAILRLAVPYTGMILTAREPAFVRDEILEFG
                     VSQIDGGTNIEIKGYTKDEAEGQNLDKEQFQINDNRSLNEIINELVVKGMIPSFCTSC
                     YRAGRTGEHFMEFSVPGFIKRFCQPNAILTFAEYLQDYAPEDTKIAGYALIEKEITEM
                     EDASLKQKLVEKLEAVKTGKRDLYF"
     gene            complement(27454..28161)
                     /locus_tag="PK252_07750"
     CDS             complement(27454..28161)
                     /locus_tag="PK252_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT14641.1"
                     /translation="MHSVSPEITFQENLKIQQKDIEKYHKVTAIKHLTKDAFIISFER
                     KNKTFKAGQHIRIKLPQYSSFRVYTIYSGTNSRYFEVLVKEVEDGVLTPKLKHLKIND
                     YVHIEGPSGHFCLDQEDINKTKYLFLASGTGISPFHSFTQSFPDLNYHIIHGVRYIED
                     AYEKEHYPKDKITICTTGDNSGGYHGRLTQYLHDSKIDLDQKVYLCGSSAMINDAIEI
                     LKKKGFQLSQIFIESYY"
     gene            complement(28281..29291)
                     /locus_tag="PK252_07755"
     CDS             complement(28281..29291)
                     /locus_tag="PK252_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT14642.1"
                     /translation="MAKSEYIWAIGDIHGYNQSLQAVLNAISSYNNKKLIFVGDYIDR
                     GPEPKDVINTITNIKEEKVTLLGEHEFLLLNAIEGEASNPKAVLEWSKHGYENTLKAY
                     EAHDVESLKAQIDKHHLEFINNLQLFHTETLGSGKKQLNLLFSHAGPFLDFPLEEQLK
                     IQHYKDFRNYINSKNIAIEENCLNNDDRLLQKSLSVWDGYLLVHGHFRTQYRQNKNRL
                     QYASKDKDYNYDMSDIPNPVYFPGGGAVAALCIDTGVDIGGKLTAVGFSEENIDFNSG
                     KMLLKVVQVDSSRRTKSFHPVTFELTLPFTDEISWLAKLKKSLFHSKKKKAKPSGHQH
                     HH"
     gene            29447..30046
                     /locus_tag="PK252_07760"
     CDS             29447..30046
                     /locus_tag="PK252_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOT14643.1"
                     /translation="MENDVFYTIKRPAEGVFKDKGSKFLAFAFPIESEQEFKDHLARL
                     KKQYFDARHHCYAFAIGINREINRSGDDGEPSGTAGKPIYNQILSSNLTNIAIIVVRY
                     FGGTLLGTSGLIQAYKGAAADAIANAEVIEKHVQAKLNLSFEYELLNQVLKIVKGEGA
                     DILNQQYDNTCTLEVSIRASKLELLSNKLEKIPGVRIEH"
     gene            30155..30703
                     /locus_tag="PK252_07765"
     CDS             30155..30703
                     /locus_tag="PK252_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14644.1"
                     /translation="MKKALIIVGSIIGLLVLVVVIYLAVMGMFSSPKVTEKEMGPYCF
                     VYEDFTGPYQNTGPVFDKVYKAMTDAGIKSTLGIGVYFDNPAEVPAEKLRSQCGAIYD
                     IADSAKVAKLLPTYKLGKIEKKTFVVVEFPIKGAMSYMIGPAKCYPVLDKYFTEKGMK
                     AAESFEIYDMPNKVTYYIMAGR"
     gene            31681..33189
                     /locus_tag="PK252_07770"
     CDS             31681..33189
                     /locus_tag="PK252_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT14645.1"
                     /translation="MEHLPKLIEDLALIMIVGALVTLLFKKIKQPLVLGYIIAGFFVG
                     PHLSLVPTVADTENIKTLAEIGVIFLLFSLGLEFNLKKLLHIGGASSITALVEIVFIT
                     LTGYMLGKFMGWTTMDSIFLGGMLASSSTTIIIKAFDDFGIKTRQFAHVVFGVLIVED
                     IVVILLLVLLPTISVAQQFAGVDMLYIVLKLLLFLVMMFVSGIFILPTLMKQMRKFLN
                     EETLLVLSVGLCLGMVVLATRIGFSAELGAFMMGFILAETSSAEKVEQVIKPVKDLFA
                     AVFFVSIGMMIDPAIIIEYGWAVIAVTLLTIFGKLISTTAGAILSGQSLKQSVQVGMS
                     MSQIGEFAFIVATLGLSLGVISEFLFPIAVGASAITTFTTPYMIKYSDKFYGFLERSL
                     PAKFLESVNNYSLSTQSIKSESRWKSVFKSYMFIVLSNSIIAFSLLFLLVEFLFPFLK
                     EVFPHQVIFGVVSLIVSLGVISPFLWGIMAKRPGDIAYKEMWVNEKYNRGLC"
     gene            33183..33944
                     /locus_tag="PK252_07775"
     CDS             33183..33944
                     /locus_tag="PK252_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT14646.1"
                     /translation="MLILEIFRVIIGLLIVGFLVNAVFTTVVALLILVPFILVVLFIF
                     SNHIKKFYQRLEDRFLNNLNTQKSHQDKDDASINTQKQISQYLDQTPWNIHIIELEVG
                     PHATFIGQTLESLSWREKFGINVAFIKRGEEMIYAPSRISRLFPFDKIGIIATDEQML
                     AFSAVFNEVDKINCVKHKIEDVGICAVQINEYNKLKGLSIKDSRIREYTNGMVIVIER
                     NKQRLLNPVSATVFEWGDIVWIAGERSKIQKLNQR"
     gene            complement(33935..34471)
                     /gene="cobC"
                     /locus_tag="PK252_07780"
     CDS             complement(33935..34471)
                     /gene="cobC"
                     /locus_tag="PK252_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HOT14647.1"
                     /translation="MKLIAIRHTTVNVPAGLCYGQTDVDTANSYDTEKNEIVSKLKNE
                     SFQAVYSSPSLRCRKLAEDIANGTPVTYDSRLLELNFGSWEGKTWDEITPTTEANNWF
                     KDWINQPCPNGESYRQLLQRVKEFTTFLKQAHTNETLLVVTHSGVIRALSCLLTGLDP
                     NKSFEIKVDFGSITQLYL"
     gene            complement(34495..35277)
                     /locus_tag="PK252_07785"
     CDS             complement(34495..35277)
                     /locus_tag="PK252_07785"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF001277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOT14648.1"
                     /translation="MIKRELHILLTAIMFYTRIKVPKNMAFSDEMLNAATRYFPFIGL
                     IVGGTGALVFWTLYMFLPLTLAIIFSMATTVLLTGAFHEDAFADFCDGFGGGYTSDRI
                     LEIMKDSHIGTYGAVGLIFMLLSKFLCISSFSIFEIPIMLVTAHAFSRFMPVCLIYTS
                     EYVRKDALSKSKPIGHKGSITTFIVAGIFGTAGFFFISWKEALVILALSLPLFIYFRW
                     YVTRKLGGYTGDVLGALQQLTEIAVYVGAIIAKQAVSISSPL"
     gene            complement(35279..36277)
                     /gene="cobT"
                     /locus_tag="PK252_07790"
     CDS             complement(35279..36277)
                     /gene="cobT"
                     /locus_tag="PK252_07790"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOT14649.1"
                     /translation="MKNALQHKVDFKTKPLGSLGKLEDIAVKIGMIQNTLSPELKNPT
                     HIVFAADHGLADEGVSPYPKEVTYQMVMNFLSGGAAINVFCRQNGIDLKVVDAGVDFE
                     FPQGANIVHAKMGRGTKNIMKEPAMTVELCQKTLDKGRDIVNAEFKKGCNIISFGEMG
                     IGNTSSASLLMNKYTGIAIEDCTGKGAGHDDEGLKRKIAILKAASNKHNVSSPLEILA
                     TYGGFEIAMMCGAMLQAKANGMIIIVDGFIVTSALLAAYNMDKTILDNCIFCHCSGEK
                     GHKAMLDYLGGVALVDLGMRLGEGTGAAVTYPLIKSAVLFLNEMASFESAGVSNKE"
     gene            complement(36365..36919)
                     /gene="cobU"
                     /locus_tag="PK252_07795"
     CDS             complement(36365..36919)
                     /gene="cobU"
                     /locus_tag="PK252_07795"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOT14650.1"
                     /translation="MAEIIFITGGQRSGKSRFAQQLAEQKSANPVYLATARAWDADFK
                     ERIKRHQADRGNQWSTIEEEKHISGLDLHGKTVLLDCITLWLTNIFHDNEYKVEEALT
                     QAKEEWTKFIQQNFTLIVVSNEIGMGIIPEHESARKFADLQGWMNQYIASLANAVYLM
                     VSGIPVKIKTTPTPSRLRREEIQH"
     gene            complement(36926..37732)
                     /locus_tag="PK252_07800"
     CDS             complement(36926..37732)
                     /locus_tag="PK252_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT14651.1"
                     /translation="MAYFFEIENLSCGYDDKFHISNINIGITKGSFVGIIGPNGCGKT
                     TLFKGITGELKLKSGKIKLNGINLKHLSHKEKARNIAIVTQDIETPYITVEDYVLMGR
                     IPYHKSYQFFDTKDDYDIAEKYMNLTDIAHLRNKYLTQLSGGERQMVAITRALVQEPE
                     LLLLDEPTSHLDITHQIQVFNLVQQLNEQLGLTVLMIIHELNLAGEYCDQLILLNKGE
                     IQQKGSPEEVLTYENIEKVYNTVVITKINPLSGKPSIFPVSQKTLNKMKE"
     gene            complement(37736..38755)
                     /locus_tag="PK252_07805"
     CDS             complement(37736..38755)
                     /locus_tag="PK252_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT14652.1"
                     /translation="MSISKNIKWGLWILAFLLLTILSVLISLSIGELHISLTDIPRIL
                     FNGKGSMEYTVLRDIRLPRIYIGLSVGGALSIAGAILQGVYRNPLVEPYTMGISGGAA
                     LSVAFIIVTGLSLRVGAFLLPLAGFIGALATIFLVYFLSVRNGKVLTQNMVLVGVMIS
                     FIASSGMMLLMSYTTSDNIQSIVFWIMGSLDEPNSSLIALGVTMSVAGLAVSYLFIHP
                     LNALRLGEDKARHLGINTDVAIKILFILASLLTGMCVAVAGVIGFVGLIIPQLMRLLT
                     GSDFRILLISSFLAGGSFLVFCDTVARTIIAPNELPIGVITGIIGGLVFIVMLSRSST
                     KAKLS"
     gene            complement(38752..39561)
                     /locus_tag="PK252_07810"
     CDS             complement(38752..39561)
                     /locus_tag="PK252_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT14653.1"
                     /translation="MKLKPGYILFFVFAILSLNTFSQRIVSLAPSLTKMLYILEAEEK
                     LVGCTNYCIEAIKDKKSIVITGMEINTEKIYTLKPDWVITTDLTKPTDIAALKKIGLK
                     VKVMPSPKSYTQICEQLTELARLTNKQSKAKSIIDQQQARLDRLKSLIPKDKKPKVFY
                     EIGAKPLFTVIPNTFMDDFITYSGGQNIAADLKQGTITREAVVLRNPDFIFIVTMGVV
                     GDEEKAAWLKYSNISAVKKHKIFIVDSDKACSPDPVSFVDVVEQLIKTMYP"
BASE COUNT        11606 a         7230 c         7932 g        13006 t
ORIGIN      
        1 ttatatccat acctgattac actcatggcg caaccttttc ctgaacgcta agcactgtgc
       61 caggttttaa tttgtattga taaatgcaac tttatatggt gttttttatt atttttcgtt
      121 tttaatccat tgggcaattt cttctgtaac tatttttcct gaccttttgt ccagatgaac
      181 accgtcagta tagctaaacg tttcattttt aggggttaaa tcaaggtatc cacatgtttt
      241 gtctgctatc tgtttgatct tataatcgaa attgggcata cattggtgtt ctatgtccat
      301 caggccctta gaaacaggta accttactaa atacacactt ccaaatttgt taagaaattc
      361 aatagttttt accagatagt caaaccttgc ctgagaatat ttatagttgg acaggaaatt
      421 tttatactcc tgtaaggtac tttttgttct tctgtttact gatacagaat ctgtattgat
      481 tgacacttca aaccatccat tttcatgcag tacggcatct gaagatttga atatgatcct
      541 gtaataatta ccaaacatgt acttgagcaa gtagtgcaaa ttaggttttt ggtcaacaat
      601 ttttatttgc cctatgaatg aattgttttc tttgaatttt gttgtatcgt ttggattttc
      661 gcccatgcaa gatatgctcc aacaatcaac agtaacaatg aatatgccgc tatggctttt
      721 ttgagatagt ttgcgtttta tactttccag gtatttaggc ccaaaaggac taatattcat
      781 attaaaagaa tagttataaa tatcgttccc taagatttta tttgcaacat caggtatgat
      841 cccttgtgca gcttttgaag tccctaaaat caggctattt tgtctggggg tagtaaattt
      901 tagataatat gggtcagaat aaccatcggc ttttaataaa ataagatata tcgaaaccag
      961 gataagacaa gtaaataaaa tgatatatat tataaacttt ttcattgttt tcaaaattga
     1021 aaatatatga attcctggct tggagagcca aacaaaaaga taatcatagc aatgaggtaa
     1081 taaaaaagcc attgtaatgg tttgtcccaa tgcgaggcta tttttatggc atattgctgc
     1141 tctcgtccta tccattcaac tataaggaga aaaataataa accccactgt tattgctgac
     1201 tctttgatac caagaaaata tggcatacta aacaaattgg aagaaaatat ccctgaaatg
     1261 taatttattg catgttccag attttgagac ctgaagaata tccaagccag aactgtcagg
     1321 ctaaatgtga caatcatagc taacaactct ttcagattgg gtattagtct gccctgagca
     1381 actgtttgta aatggatcct gttcctgtca gttaataaca atggcaaaaa atacaaggca
     1441 ttcaaagccc cccagacaat aaaagtccaa ttggctccat gccagaaccc acttacgatg
     1501 aaaattataa aggtatttct gactttcatc catgtcccgc cgcggcttcc gcctaatgga
     1561 atatacaagt aatctctaaa ccatgaagat aaagaaatgt gccatcggcg ccaaaactca
     1621 gcaatatctc tcgaaaaata aggaaaagca aagttgcgta ataattctat ccctaaaagc
     1681 cgggctgttc ctatggcaat gtcagaatag ccagaaaagt cgcaataaat ttgaaaggta
     1741 aaaaacaatg cccctaaaac aagggtactt cctgaataat ctgcctggtt gttgaaaatc
     1801 atgttggcat aaatggcgca attgtcagca attaccatct ttttaaataa tccccataag
     1861 atttggcgaa gtccatcaac agccgtataa taattaaact cccgtttttt cactatttga
     1921 ggcaaaaggt gagtagccct ttcgataggt ccggcaacta acaatgggaa aaagcttaca
     1981 aaaaccgaat agttgacaaa attacgttcg ggctttatct tgtttttata aaggtctaaa
     2041 acgtaagaca gcccatgaaa tgtataaaat gatataccta ctggcaatat tactttgata
     2101 gacccaaggt tagcatttat ccccagtagg tgaagccctt gggtaaatga ttctgcaaaa
     2161 aaattataat atttaaaaac gcccagaaac cccaggttga tgcagatact aagccataac
     2221 caaaataact tatttctttt tgttgtggca tcatatattt tgatacctgt aaaataatca
     2281 agtgaggttg aaaatacaag caaaaataag aacctccaat cccaacaggc ataaaaaaag
     2341 taacttgata ttaataacag tatattttgt agattcaggt tcttattggt aacaaaccaa
     2401 taaagggtaa aaacgattgg aagaaatatg gcaaagtgta tcgaattaaa aagcattttg
     2461 tctgtccgtt cagttttaac aattaaaagc ctctttgcaa aaatgtgaaa taatcaaatc
     2521 aaacacaaat aaactatcag gcaaggggca aaaggtatat atatttgatt ttgttgaaga
     2581 atattggcac ctcaaattta gcatcacctg taccttgcgt aaaaagagag gttgaaattg
     2641 ataatactta ttaattctac tttgacagat tagttaacct ttgctattag ccgcttagcg
     2701 gcgaaatctt tgtagaaaaa ggttgctcat aattattaag ccacgtagtg gcgaaatata
     2761 taatatgcta tatcggtgct acgcacctga tgactatggc aattttattg agctactaag
     2821 atgtcgcagc tacgtggctt ttttaccttc atattttaac tgtatgtaat tgaatttcaa
     2881 tatatttaat tagtcaaagt agagttaaat ttcattcgta atttattgtg gttggtgttc
     2941 tttgcggagc aggtcagcgt aagtttttac agggttgaag gtaagaatag gcacttctac
     3001 cattagggta atccaatgcg ccatacctcc gttccacaga ccgggcaatt caagggcttt
     3061 tacttttttg cccgatactg atttttctga aataaaacca gcattgttat ctctgaacct
     3121 tagtaaatta aacttaaatc cactatagtc ttttgttgag caaaccaaat caaccgggtt
     3181 gaagtgagta gccccgtcaa atatcacttt ttgctggtgg ttgttcatat ccacctgcga
     3241 tgattcaact atttggaggt ttgcttcacc ctttttgttt aacacccaga aaggccctcc
     3301 gccgggttcg ccaacgttac gtaccatacc acagacgcgg atgggtttgt caagttttga
     3361 ttttacataa gccgcaaata ctgagttatc cataccttta ggtaaggtaa tgcccatata
     3421 ttttagcata aacgtagcaa tgcgttcttt caatgtttcg ctaatggtgg tattagcctc
     3481 gaggtagttg atgtattcgt tgatttctgt ttttacaaaa accaacaaac ctgccaaagc
     3541 ctttttatac ttgactgtaa catgcttcag atggtcaggc acaacgttgt cgatattttt
     3601 gataaaaata aggtcagcgt caatgtcatt taggttttcg agcaaagctc catgtccccc
     3661 gggcctaaat acaaacttac cgtctttgtc tgtaataggg acgttattaa catctacagc
     3721 aacggtgtct gttgattttt tctggaaaga atacgaaata gaaagctctg cttctttgtc
     3781 atattttgct tttagttttt caaattcagc aataaaaagt tgttcgtgtt caacagaaat
     3841 cgtgaaatga agttgtagtt tatttgcacc ttttgcatac ataagccctt caacaaaatg
     3901 ttcttctgta gctgttctgg tttcgttgtt gtatgcatga aatttaagca gccctttggg
     3961 caaactgcca tagttgagac ctttgtttgt taataaagtt tctacaacaa ttttggtatc
     4021 gtcaacattg tgcatagtcc gtatatcata acccagcttt ttcaacgctc ctgaaagatc
     4081 ctgatagaag gcaaaactgt cgatgcttcg catcattatc gaacctccta cattggctga
     4141 agggttatct aaaaattcgt acaggttttt aaacatgcgg ctggcagctc ctgatgctgg
     4201 tacaaatttt aatattgaag ctgttttgct atattcgtca taaagctttg tccatttttc
     4261 tatttctttt tcggaaggta ctattatccc atcattgatg gtagctggcc tgacaagggc
     4321 tgcaaattca aatcctttaa tgaagctttg aatttgttgg ttaagctgtt cttcgcttat
     4381 gcccatttct tctaattgtt ttttttgtat cggggttatc atagttgtta gttaatagtg
     4441 tttaggggat agaggattct atttcttttt ctccatagat atttttctgc ccaaatcaaa
     4501 ggctttaatg ttcagattga ccatttcttc gcttttacgc ccgaaaagtt tttttatccc
     4561 ttctataaag ctttcataac tcatttcgat atatggagaa gctgccccaa gcattacaat
     4621 gttggatgat tttacagacc ctgcttcttc tgcaatttta tcggcatcaa ttagtacatg
     4681 gttaggtatt ttgttgattt cgtccaatat cttctgatat tcagggtagt tggggatgtt
     4741 aataaatggt aatgagttgg tgataatcca accatttttt gaaagatatg gaagatacct
     4801 gaggctttcc atgggttcta ccgaaaggat aacatctgcg ctgcccagtg gtattaaatc
     4861 tgaggctata ggattgctgg ataagcgcat gtgcgattga acggcacctc cacgttggct
     4921 catgccatgt acttcacttt gcttgagatt taaattgtcg tgcagggctg catatccaat
     4981 acttgctgca atggttaata taccttgtcc tccaacccct gctaatatga tgtctgtttt
     5041 cacttttttt cagtatttaa gttaatttct atgttttctt gaaagtgttt ggatacattc
     5101 gcggcgcccg ataattacag aaacaccttt gtactcaagt tcttttttca aaagcgccac
     5161 ggcctcttca tggtttttct tcagcgggtt gaacactacg atgtgttctt tttctacccc
     5221 aaggccttcg catatttgtt caatgcgacc tgttgcagaa gatttctggc ctccggtcat
     5281 acctgttgtg gcattatctg caatgataac tgttatacct acattgtcat ttacagcatc
     5341 taacaaacct gtcattcccg aatgggtaaa agtgctatcg ccaatgacag ctactgcagg
     5401 ttgcaatccg gcttcgtatg cccctttagc catagtaata gaagcaccca tgtcaacgca
     5461 tgagttgata gcgttgaagg gaggcaatgc ccctaaagtg taacatccga tatcggcaaa
     5521 tacccggcct ggggtatagt cttttaacgc ctcattgagg gctgtataaa tgtcaatatg
     5581 tgcacagcct ttgcaaaatg aaggaggacg attggttacc acttctggta tttctgtaac
     5641 tttgatgttt tgttttaacc ctaatgctgc accaacaata tctggggtaa gttcgccatc
     5701 acgcggcaat atgccattca tacggccatg gatacgggtg cctaccccca taattcccct
     5761 taattgctct tctacaaaag gataaccgtc ttctaatacc aaaacagttt cgcattcgtc
     5821 atacacttgc gagattagtt gttgtgaaat ggggtagtgg ctaagtttta aaaccgggta
     5881 agggatattc ccgtcaggaa agttttcaag gaggtagttg taggttattc cgcaggcaat
     5941 aatacctata gatttgttgc tatgattggt aaatttattg aaaggagagt tatacgctat
     6001 ttccatcatt tccttttgct gtcccagcag gcttttgtaa cgttgacggg ctatagccgg
     6061 caataacaca aagcgttttg aatcatctgg tatttgggta tttttctgag cttttgcagc
     6121 acgccttaca ataccagccc ttgagtgtgc cagacgtgtc acaatgcgca ccataaccgg
     6181 gagcttgaaa tattcagaaa ggtcaaaggc ctggtaaaca atatcgtatg cttcttgctg
     6241 atcggcaggt tcaaacattg gtatcatggc aaacttacca taaaaacgtg agtcctgctc
     6301 gttttgcgaa gagtgcatcg atgggtcatc tgcaacgaca ataactaagc ctccgttagt
     6361 acctgtgata gctgaattga taaagccgtc ggctgcaaca tttagcccaa cgtgcttcat
     6421 acataccaaa gctcgttttc cggcatacga catgccaagt gcagcttcat aagctgtttt
     6481 ttcgttggct gcccactggc tgcggatttt gccacttttc ccttcttcag aggcttgtat
     6541 gtactctgta atttcggtag aaggggtacc cgggtatgca tataccccag agaggcctgc
     6601 atcaatagct gcctgggcaa tagcctcatc ggcaagaagt aatattttgt ccatgtatat
     6661 tttttgttct caaaagtatt aaattctgaa cattttttgc aggattcttt gatgtagtct
     6721 gcttttttcg atatttacct aatactcagt acaataattg gctttttttt tgtaatttta
     6781 ataatcaatg aagtgaaatg aagatagttt gttaatcaga attatgaaca acatatatta
     6841 aaagtattat tgttattctt cgaagggggt tttgtttttc tgacttattg aaaatattta
     6901 tggatggaac atccttgctc ttgtgaatgc aaacattccg tcagctatcg ggataattaa
     6961 tattgcctgt atattcaaac acttataaac aaaatttctt tcaagggtgt tgtattcatt
     7021 tttattacgg atgaaacgtg cataattcaa acacaaaaaa acaggataaa attcatcatg
     7081 cgagttccat ccggccttta aaaattgatt ttatcatgat gaaaacaaaa ttgtatgaat
     7141 gttagaatat tacttcgacg ggtagctgca ttagaccgtg ggttagattc ggatcacctg
     7201 ctacttattg ctaatctgct tggggagctt tttatgttta ttagctgctg ccatcaattt
     7261 ttggttgggt tttgggtggt tagtttcaat ggcttgtttt attttgttct ttgttttttt
     7321 ggggatatat tttttagcct tgaaaaaaaa gtttattcaa tttgctaaat ggtctttatg
     7381 tatttctaca ctggttatac tttctgttat ttggtattac aataatggat caaacgggcc
     7441 tatcccgctt attcttgtgg cggctttttc tttgctaatg tacatgtttg tgggctggca
     7501 aaggtggatt gttcttactc tttacatatt agtacttgct gttcattttt tgatagaatg
     7561 gtactttcca gggaggatta aaccatatcc tgatgaaaag acccggttga tagattttta
     7621 ttcaagtgct gctatgtatg tgatgcttat gagtttaatc atgatatata ttaaaaatat
     7681 taaccaacaa caaaaagaaa aggctgatag ggctaatgag ctaaaaacaa agtttttgtc
     7741 aaacatgagc cacgaaattc gaaccccgat gaatggtatt attggttttg cccagatgtt
     7801 aaaaaaaact gggttgacag aagaaaaacg tgagagatat gttaatgcta ttcttaccag
     7861 ttcaaagcag ttattaaata ttgtgaacga tatacttgat atttctaaga ttgagtctgg
     7921 gatgatcagc ctttcattaa ggcctgttaa tattaagaaa gtgattttta atgttatcgc
     7981 aaataatgaa ttacttgccg ttcagaaagg tttacaattg ttatataata tagaaaatta
     8041 taaggaaaat gaactttttc tggctgatga acaaaaactt gggcaggtgc ttaacaatct
     8101 tgtaaacaat gctttaaagt ttacttcaaa tggcagtatt actattcatt gttgggtgct
     8161 tgagaaagaa ttgaagtttt gtgtagaaga cacggggagc ggaattgaag agaaatatca
     8221 gggcgctatt tttgaccgtt ttcaacaggt aatgaatgga aataaggaaa atgttggtgg
     8281 tacagggtta ggccttgcta tttgtaaaaa ttttgttacc ataatgggag ggaagatttg
     8341 gcttgagtcg gttcctgaaa tggggagtaa attctttttt accattgaaa aaaagattcc
     8401 ggaagaaagc agtactacaa aaaacaatga aaaagacatt aaaagggttg ataatttaaa
     8461 gtttaaatca acaagcatat tggtagtaga agacgatgtt aataattata acctattgaa
     8521 ggaaatactt gccgaacggg gggttacaac cttttgggcg caaaatggca aagctgcaat
     8581 aaaaatggta gaagaaaacc ctcatattaa attagttttg atggatatta aattacccga
     8641 tatgactggt gttcaggctt tgtttcaggt gctgaaaata cgccctttgc taaaagtcat
     8701 tgcaaccaca gcctatgctt ttgaagaaga aaaaaaagaa ttgatgtcgg ttggttttgc
     8761 tgattatgtt tcaaaaccca ttaaagtaga gttgttgttt aaagcattga atgatagctt
     8821 gagttaatgg ttaatggtta atggttaatg gttaatggtt aatggttaat ggttaatgaa
     8881 tgagttggga ggtttttcta aaatttgtta aaacgcgggc atatgagcct gttttttatc
     8941 aaatttgcat taaattaggt gggtaatgaa acgcaacata tttataggat tgttcatttt
     9001 atttagtgtt ggattgcttt cacaatctaa aacagcctat actgttgggg ttaacagcct
     9061 tggaaattat gggcttaagg tggacagggg agattcactt tctttcttct cgtttagcta
     9121 tacctcgttt aattttaata atacctttaa ttcgaaaggg ttttcaatga atgctccgtc
     9181 attgggcaat tctaatatgt ttgatgtggc atatttcagg aataaagacc ttagtaagcg
     9241 attgacattt gtttacggag gagggataag ccttgtgtcg tttcctaata ttaccagctc
     9301 atctgttgtt gtgaaaaatg gcgggtacaa gccaaatttc gatttaaacc tcgggttggt
     9361 ttataaaata tctgataaag taagcgtttc tgcttcgttt aatcagcgat atggttattc
     9421 tccctatttt tatgataatt cattgtttgg caactctttt ctcagtcctt ttcataatgc
     9481 tggttttaat tcgttccaca attcaccttt tgacccgttt tgagaagatg aattacgaat
     9541 taaaaattac gaattacgaa ataatgtaag ttgagaacaa acttcgtaat taattaaata
     9601 tctgttatat tgtattagca tgtttcctcc tttgagagaa gaaagtgtaa aaggtgttaa
     9661 ttctacgttc gacctgacag cgtttgcaga acctgtcaga gtcataaacg aaattgattt
     9721 ttaattcgta atttgtcatt cgtaattgtt tttttctgcc ataatttcac ctttgcaacc
     9781 tgtatatttg tcagttattg cgcttggcac atcatttgaa aagcagttat cagcaaaaag
     9841 aagtcaaact taaaaattaa ctgatatgtc taaaggtaag ataggagtaa gtacagaaaa
     9901 cattttcccg attatcaaaa aatttctcta ttcagatcat gagattttcc ttcgtgaaat
     9961 cattgccaat gccatggatg catctaataa gcttaaggcg cttgccagta ctggtgagta
    10021 taaaggcgat gtttcaaacc tcaaggtttc agtagctgtt gacgaaaaga aaaagactat
    10081 aaccatttct gataatggta tagggatgac tgccgatgaa gtagaaaaat atatcaacca
    10141 gattgctttt tccagtgccg aagaatttgt tgagaaatat aaaaaccagg ctaattctat
    10201 tatcggtcac tttggtcttg ggttttactc agcttttatg gtttctaaaa aggttgaact
    10261 ctttacccgt tcatataaag aaggttcaaa agcagtacgt tgggaatgcg atggcagccc
    10321 cgaatacatt attgaagata ctgaaaaaga aaccataggt acagatgttg tactttattt
    10381 agatgacgaa tcagctgatt ttgctactca aagcaaagta aatgagctgt tgaagaagta
    10441 ttgcaaattt ttgcctgtgc ctattgcttt tggtaaaaag aaagagtgga aagatggcaa
    10501 agaggttgag cttgctgaag acaacatcat caacaaccct acaccagcat ggacccgcaa
    10561 gccatcagac cttacccacg atgattacat gaaattctat cgtgagcttt atccaatggc
    10621 cgaagaacct atgttttaca ttcacctcaa tgttgactat ccttttaacc tcacaggtat
    10681 cttatatttc cctaaaataa agaataacat tgaaatcagc cgtaacaaga tccagttata
    10741 ctgccgtcag gtgtttgtaa ctgactctgt tgaaggcata gtgcctgatt tcttaacctt
    10801 gttgcatgga gttattgact cgcctgatat tccgcttaac gtttcgcgtt cgtacctgca
    10861 aagcgaccag aatgtgaaga aaatatcaag ccatattacc aagaaagtgg ctgaccgctt
    10921 acaagagttg tttaacaacc agcgcgaaga ctttgaaaag aaatgggaca acctcaaact
    10981 tttcatcaat tatggtatca tcaccgatga aaagttctgg gaaagggctg aaaagttcac
    11041 tttgctcaag aatgtaagtg acaaatactt tactttcgaa gaataccaaa agattatcaa
    11101 agaaaatcaa actgataaac atggaagcct ggtttatctt tatgcttctg ataaagaagg
    11161 tcagcactcg tttatagagg cagctcaaaa caaaggctat gatgtacttg ttatggacgg
    11221 gcagcttgat acacacttca tcaaccatgt tgaaagcaag ttgaaaaaca gtcgatttgt
    11281 ccgtgttgac tcggatatag ccgataacct tattcaaaaa gaagaagcaa aagaggttaa
    11341 acttacccag caacaaaaag atatgctttc acctgtattt aagagtgtga tgccttcaaa
    11401 tggccagtat tatgtatctt ttgaagctat gggcgaaaat gatatgccag ctgtcattac
    11461 ccgtaacgag tggatgcgcc gtatgaaaga tatgtctgca cttggcggag gaatgaattt
    11521 ttatggtgaa atgccggaca attacaacct cattgtcaat gccaaccatc ctttggtcaa
    11581 taaggtgctt cagcaaactg aagaagcaaa acaagctgaa atcaaagaaa ctgttgaaca
    11641 gattactaag ttaactgctg aaagagaggc tttgatggca ttgcacaagg ataagaaata
    11701 tgatgagata cctcaggctg agcgtgataa ggaagctgaa ctggacaaaa agattgatga
    11761 gttagaggat aagcgaaata aagtgcttga agaatatggc aaaaccaacc ctttggttag
    11821 ccagattgct gatttagctt tacttgccaa caatatgttg aaaggcgaag cattgagcag
    11881 gtttgtaaaa cgtagtattg aactgataaa gtagatatat agatgctttg cttttgaaaa
    11941 gacaaagcat ctgcgaaata aaaagcaaaa ggtcgttgat gtaccgtcaa cgaccttttg
    12001 cttttggcca ttgatgatta tgcaatggcc aatgcttgtt ctaagtcggc tttaatatca
    12061 tcaatatgtt caatacctac agataagcgt agctgaccgg gtattactcc agacttcttt
    12121 tgttcttctg ggcttaactg ttcgtgggtt gttgtagccg ggtgtattat taaggttttt
    12181 gcatctccaa tattggcaag gtggctaatt aactttacat tattaacaac tttgtcagca
    12241 gcttcctttc ctgctttaac tttgaaggat aatacccctc caaagccatg tttcatgtac
    12301 tttttagcaa gtttgtgata tgggtcgctt tctaatcccg gataattgac aaattctact
    12361 tttggatgtt tttctaacca tttggcaatt tccagggcat tatccacagt gcgctgtaca
    12421 cgcaatgaaa gagtttccaa accttgtagt aaaaggaaag cattgaaagg acttagcgaa
    12481 cttccataat cgcgaaggcc ttccactctg gcacgtatgg caaaagcaat atttccaaat
    12541 gggcctgcac ttccaaatac ttcccagaat tttaatccat ggtacccttc tgaaggttca
    12601 gtaaattggg ggaactttcc attgccccaa ttgaagttac ctgcatctac tatcacaccg
    12661 ccaatgcttg ttccgtgacc tccaatccac ttggtagccg attcaacaac aacgttagcg
    12721 ccatgttcta ttgggcggaa caaatatcct ccagccccaa atgtattgtc cacaaccagt
    12781 ggaatgccat gcttttgggc aatagctgca atagcatcaa aatcgggtat attgaaacgc
    12841 ggattgccaa tggtttctat gtaaatagct ttagtatttt tatcaatcag cttttcaaac
    12901 tcggcaggtt catcgccatc gacaaacttt gcagtaatgc caagcctttt gaattgcact
    12961 ttaaactgat tgtaggttcc accgtacaag tatgaggtag aaacaaagtt atccccggcc
    13021 tgcaatatgt ttgtcagagc aaggaactgc gcagcctgtc ctgacgatac tgccacagca
    13081 gcaactcctc cttcaagcgc tgcaatgcgc ttttcaaaca cgtcggtagt tgggttcatg
    13141 atgcgtgtat atatgttgcc aaattccttc aagccaaaga gtttagccgc atgttctgag
    13201 ttgttaaaaa cgtaagatgt cgtttgatag ataggaactg cccttgaatt ggtagttggg
    13261 tcaacttcct gtcctgcatg caattgcagc gtttcgaatt tccaattttc ttgtgccata
    13321 atgttgttta tttaatttat tgtaaaagta taaatggggt ttttttccaa aataccgtct
    13381 atgtggcatt ttacatacca taaatgcgtt atggggcgtg tcagatatag tattcaatac
    13441 tgtcaatcag cccgtttttc atggcataca tgatgagctc tgaagagctg tttatctcaa
    13501 gctttcggaa gatattcttt cggtgtgtca taacagtgtg gaagctgata ttttttttat
    13561 cggcaatctc tttagtagtt aagccttcgg ctatttgcct gacaatttct atttcagcct
    13621 ttgtcaggtt agcttttact ttttgtttgc ctttgctctt ggattcgcca agaatagttt
    13681 ccatcaactc agatgaatag tactttttgc ccttataagt agcctcaaga gcttgcaaaa
    13741 aatcatcttt gtcagtggtc tttaacaaaa tatttgaaat acctatagaa tttagcacat
    13801 taatctcgtc acgactaata ctattggtca gaataagtat ttttatatcc ggacatttac
    13861 ttttcagtct ggatagatct tcgatattat ctattcctgc catttgatgg tcagtaataa
    13921 caacaccttt gtcttcaggg caaattagtt gatgtacata agcaatgtct tcagcaatac
    13981 cttttacctc aaacttatct tcgttataga taatacttac aaggccttca acaattaatt
    14041 gttgcgaatc ggctattatt acttgtattt tgttattcat aattattaaa atgctattag
    14101 aacctatatt tgtttggata acaacaaaat taagctttaa tttataaatt acccgattaa
    14161 aagaccttca attcttactt tatagctacc gtctttgagc ttggtcacgg cattaaccag
    14221 cccgctgtgg cgccattgca gcatgcaacg tcttgggagg aaccaatatc tgcggccaag
    14281 ttcaattttt tctttagatt ccttaataat tccctcataa gcctgggata cataaaatcc
    14341 aatatcaaca aatccttctg aacgagggcg caatggacct gtaacatgcc agtaaataac
    14401 gccattacga gggtcaacat cgtacactac gccacagtgt gtcaccgggc agccacagga
    14461 cattcccata ggcctgccaa ccataacatc gcctttttta acgccaagat ccttgactaa
    14521 tgaagggttg taaggcaata tagtttcttt aggcccaggc tcaccttcaa tgatgtcgag
    14581 gataaagtca aagtcgcgtt ggagactgtc tttccagtcg agctttttgc caaggttttc
    14641 ggtatgtaca cagttgatac agttgaaacg acctgtttct attgacgcca ttggcttaag
    14701 atgaatacac ttctttattt cactctcttt gctgtcaata gcttttgcaa aatcattgca
    14761 ggtgcggtat ccacatgcgc cacagttcaa acctggcaat gattctaata ctgttttagt
    14821 ttttatctct tccataatgt ttatttctaa tgtgaaaaaa ttgaatttaa ctgtaaaacc
    14881 ttttgtttaa tggttttttt gaatatttcg atatctgaat ctgttgtata acgccccata
    14941 cttaccctaa tggctccacg ggcttcaaat tgattgagcc caatggcttt aaggacatgc
    15001 gaagtgccat ctcctccatg atttgaagaa catgcagagc ctgccgaaac agcaatgccc
    15061 atttcgtcaa gcatcaatag taaacgtatc gtgtcacctt ctaatccttg aaagctaaag
    15121 ctaagatgtc cgggaatcct gttgtttgga tgcccattga ggtaaaaacc ttccatattc
    15181 tcacttaaat cgtcaataat ggatttctga agcgtgaaaa gtttgatgtt ttcattatcc
    15241 atgtccttga tagccagttc agcagctttg gcaaagcata caataactgc aatgttttct
    15301 gtagaagaac gcaagccgtt ttcctggcct ccaccatgta gaattggagt tattctggta
    15361 ttttttcgca catataaggc cccaatgcct ttggggccat atattttgtg ggcattgata
    15421 gtcaacaggt caatattgct cttgactaca tcaataggca acttgccaaa gctttgacat
    15481 gcgtcggaat gaaaaagaat attgtgtttt cggcaaatag caccaatttc agcaataggt
    15541 tgaatggttc caatttcgtt gttggcatac atcaccgaga ctaatatagt ttcaggtttt
    15601 atagcccttt ctaaagcttg taaatcaagt gttccataac tgtcaacagg aactaatgtg
    15661 atgctgaaac cttcttcttc aagccatttg caagtattca atatgcaatc gtgctctatt
    15721 gctgaaacaa tgatatgatt gcctttgttt ctgtttgcaa atgctatacc tttcaaagcc
    15781 atgttgttag attcactgcc actagaggta aaaagcagtt cgtcaggttg acatccaatg
    15841 tatgaggcta ttttagcacg agaattttct aaagcttcct ttgaccttgt accataggta
    15901 tgcatacttg atgcatttcc atatatttgg gtaaaatatg gcatcattgc ttcaattacc
    15961 ctaggatcag ttggcgttgt tgctgcattg tccagataaa tacctttcat tgctagtttt
    16021 gttgttgtta cccctccgag ggttttaaac cctcggaggg gtattgtaat ttattctcca
    16081 atataaaaca gctctttttc catagtccgc aataccccaa agtggttctt ggcgccaatt
    16141 tcctttttgc caacacaaat ggtgcaggtt ccaaccggtg ggttaccacg taagtacatg
    16201 ggcatttcaa catcttgagc ttgggttatc tggcgtacaa tcgggtcaat gcctatgcca
    16261 tacagggcat tgctttctac aaccttaatg tctttggccg cttccaacac cctgtatcta
    16321 aacacttccc gttctgcttg cgatataagg tcaatcttcg tgataacact tatatctgct
    16381 aatgttagca taggccctat tttgcgtggc aggttcatgc cgcttgttgc ttcaagaact
    16441 acaatgccta atgaattttc gatatatgga gagcatctga ggcacaaacc tgctgtttcg
    16501 acaaaaagat attctgctcc ggtgctttct gcccattcta cagcctcgtc cagcactaca
    16561 acattgcaat ggtctgggca cagttcgcct gaatatattt tgcgtgtagg aattttaaat
    16621 tcttcgcgga tggtttcgtc ctcatctgca aatagtacat ctattttaat atatgatgtt
    16681 ttctggtttt gcgcaatcat tcgtttgata acctgtctga ttactgtggt tttaccacag
    16741 gttggaggtc ctgcaaatat tattagcttc atgttcttat agtttaatta aattaagatg
    16801 ttcagctttt agttcttctc cagccctaat gatactgcgg aaatgaagga ttatgtcatc
    16861 catttttctc agattttcaa ctgctaagcg ttcttcaggg ttggcaacta ctactttttg
    16921 cattgccccg cttttcatga taatacgttt gtctgaaagc aaggctatta ttgggtcgtg
    16981 tgtaacaaat acaaaaagtt tatcatattt tttgagcagt tccaatgctt ttgtcttgtt
    17041 tatccctgca ttttcaattt catccaaaag aataattggg gaattgccta ttactactgc
    17101 atcagctatt agtagtgaac gagtttgccc gccagacagc tctgtcatgg cagtttcttt
    17161 aataatggct tcaccagtga gctggttggc aaactcgatg gtttcattca ccacgttttc
    17221 aatgttttca gctccacgta ctttagcgtg aatggtcaaa aattctccaa cgggcaaatc
    17281 gctcagaaag ttggtgtgct gtgatattag tgctattgga tgttttgagg ggtcgaagga
    17341 gaattcggca ggtattggat cgccattgat cagtatttgt ctgccagaag gggtgtttct
    17401 gttggcaaaa agctcaatat cgttgatgag tgtggttttt ccacaaccag ttggcccaac
    17461 aatactcaat atttcgccag gtttcagttc cagcgaatca acctgttctt ttgaaccgtc
    17521 tttaccttgt ccgcctttaa tttctatact ttttatatcc atatcaatat ttttcaacaa
    17581 aaatatacat taagtacacc gacatagtgt acaatagatt tgattttgta atattgcact
    17641 atgaaatgtt tcatgtttta acctgcatta ctatggcttt aaatgaacaa cacaggtatt
    17701 tcgattgcag ccagtgcctt ttcaaacagc taagctgcac acttattgaa gattctgagt
    17761 ttgaggtaat tcgtaagact tcgtatcaga tgaaatacca gaaaggtgag actattttaa
    17821 agcaaggggc taaaagtacc ggtctgcttt atttgcatag tggcattgtt aagttttctt
    17881 ttaaaactga ggatcagcga aatattatca caacaattgt gcgtggtccg aagttattgg
    17941 gtggcgctaa tttgtttttc aaggatatta atatattcag cattgttgca gttgaagatt
    18001 gcgatgtttg ctatatagac agcaatgcat tacgaagtgt tgccctaaat aacggccgat
    18061 atacgttagc tatgtttgaa agcactgcca gtatgttcca acattccatt tttaacttta
    18121 tcagtctcgc acacaatcat gtaaatggcc ggattgcttc tattttgtta tatttatggg
    18181 aacatgttta tgaaggttca ggttatgatt ttattattag tcgtaaggaa atagctgaat
    18241 ttgctgcctg ttcgcatgaa aatgttatca ataccctgtc taagttcaaa aaggaagggt
    18301 tgatagaact caatggtaag aaaattgaga ttgttaatat agaagcttta aaagaactca
    18361 gtaaaaaagg ataatatgtt atcaaagaaa accagatatg ccatgatggc tatgattaag
    18421 cttgccaccg agtatggaaa aggccctatt ttgattggcg atatagccca gagtgaaaat
    18481 attccccaac gtttccttga gaatattttg cttgagctaa aaaagatggg ttatctggga
    18541 agcaggttgg gcaaaagcgg tggatattac ttattaaagc atcctaagga tgtgaattta
    18601 gctgaaataa taaggcattt cgaggggaca atagccttac tgcactgtgt gtctgaaaaa
    18661 gcgtatcaac catgtgagtt ttgtaagaac gaagcagaat gcaagattaa atcggtgttt
    18721 aaagacatcc gggattatac tttcaggctc ttagaaggaa aaaccctgga agagcttgcc
    18781 agttctaaac aaacggatat gtaaaattta attccttgaa acaaaggatg ttaaaataaa
    18841 aaatgaagta ttttcgtcga tgtctaaaaa atgataattg agctttctgg ctgttcttct
    18901 aactatgtcg atgaatccaa ggcctgcacc gcccacttca gacaattcgc cttcagaaat
    18961 ttgtttcatg tataattcgt ttatttcttc acgacttaaa ttgtttatgt agtcaatttt
    19021 tttctgcaac gcctctcttc ggcttctttc aacaatattt ccggtagtga taccgtattc
    19081 ttctttcgat ttactcagca taaatatgcc tcgtgatata ggctttgggt cattgctact
    19141 tttgatttct gcatgtttag tgatgttttg catgcattct accactacaa aattaaccct
    19201 tcgttgtatc agccctttgt catcagcttt tgaaatactt gattctgcta aggcagagaa
    19261 agctatagta atttgctggg taaattctcc ttcatacgca aagcttattt tgtgttcacg
    19321 taagctttgc aaaaaatgct cggctatttt tataaatcgc ttggggtcgt cgtttggtat
    19381 attatttata tcaaaattca tttttctgct ggtcaatttt gcttgtattt cttttcaaag
    19441 atagaaagaa taataagaaa aaaaatgcgc ttggttatgg atgaaagaat attccatctt
    19501 aaaagttttg gaagtttaat acatttgccc caatcatgcg tatctttaat gataaatact
    19561 aaaagagcat aacatcttat tacatggaca aatgtcgcaa aatattaatg caatactggg
    19621 gttttagcca attcaggccg ctccaggaag atattattgc gtctgtactc gaaaagaaag
    19681 acaccctggc gttgatgccc accggggggg gcaaatctat tacgtttcag gtgcctgcat
    19741 tggcgatgga cggcatttgc attgtggtta cgccacttat tgctttgatg aaagaccagg
    19801 tggaagggct gaaaaagaaa ggtattaagg ctgtggcggt gtattcgggt atgtctaaag
    19861 aagagataga tatagcgatg gataactgca tttatggtga ctttaaattt ttgtattgct
    19921 ctcccgagcg cttggggact gaaatatttc gtatgcgact tccaaagatg aatgtcaacc
    19981 tgctggccat agatgagtcg cattgtatct cacaatgggg ttatgatttt cggccttcat
    20041 atttgaaaat tgccgaaata agggagataa tacctgatgt cccggtgctg gctgttacag
    20101 ctactgctac tcctatcgtg gtggacgata ttatgaagca gttgaagttc aggcaaccca
    20161 atgtgttccg aatgagcttt gagcgtaaga acttggttta tcttgttcgt gaagtggaag
    20221 ataaaataag ctatttgcta aaggtgtttg gcccaacacg aggaagcggc attgtttatg
    20281 tccgaaacag gatgaaaacc aaagaaatat cagacttttt gaataagaat ggtatttcat
    20341 ccgactatta tcatgccggg ctgactgacg aactcaggag ttccaagcag gatgcatgga
    20401 agagtgggag agtgcgtgtc attgttgcta ccaatgcctt tggtatgggt atagataagc
    20461 ctgatgttcg gactgtggtg catgttgatt tgccagattc gccagaagcc tattttcagg
    20521 aagcaggacg tgctggtcgc gatgggaacc gctcttttgc tgttttgttg tacaatacca
    20581 atgacaagct cagtattagc cagcgtattg aaactaactt tcctgatttg tttgaagtga
    20641 agacggttta caatgccctt ggcaattttt tccagattcc ctacggctct ggtaagtata
    20701 tggcttatga tttcgatttg gcgaagtttg taggtacata ccggatgaat gtacagaaag
    20761 catatagtag tctgaagata ttggaacaag aaggctatat agaagttaca gattcaatca
    20821 acaatccttc caaggtcaag tttctgcttg gtcgtgacga tttgtaccgt tttcaggtag
    20881 ccaatgaagc ctttgatgct tttataaaac tgttgctcag gtcgtacgaa ggcctttttt
    20941 caagctatgt gagcattgat gaatcggtat tatctaaaag ggctaatatt ccgcttgata
    21001 gtgtatataa gtacctgaat aaactgtctt ctgctaaaat actgcattat attccccgtc
    21061 gaaacaagcc tgtggtggtt tataccgaag aaagacttga ggataaagct ttgctgctgt
    21121 cggtagaaag ttatcgcttg cgaaaagacc gctacgtgga aaaagctgag accatgcttc
    21181 attatgccac agctacaaac aaatgtcgta gtgttatcct gcttcaatac tttggccaga
    21241 atgacgctta tagatgcggg cagtgtgacg tttgcctcaa gcgcaacgag gcagatgtaa
    21301 gcaagtatga atttgacctg atagtgcagg atataaaaaa cctgcttcat gagccacttt
    21361 tattagttga tatttctaaa aagcttgaac agcgatatgg ctcggagaag ctaactaagg
    21421 tcatccgcta tttacttgac aatggtaagt tgaaatataa tgaagaacag tatttgcaat
    21481 gggtgcatta ataaatccta tttcttcacc attttcaaag gaaatggcaa attgttgaaa
    21541 tgaaagtgag gaaataaagt cttttcagct ccaaaacctg cgtagaacct tgctaatgat
    21601 tcgatacgag aaccttcaaa gtcaattact tcatccttat tggcatgtaa aaggatatat
    21661 tggtaaataa gccagaaatt ggctcttttt tctttacctt cgcttgaagt agcaggtagg
    21721 atataggttc gccttttgtt aaagttgaac cataaagcca ttgacagaat attcttatct
    21781 tcatcagaag tggcaactat ttcagccata ccacgtttga aagcttcatg atataaatga
    21841 taggcagatt ttaattcttt tttgccaaac ttaacaggta aatgcttatc aataaatttc
    21901 aaaaagaagt ctatatctgg tatagaagca taattgagtt tgtattcttt agctttctta
    21961 atattgcgta catggtttga cgagaaggca ttctcaatat cagttatagt tttttctaaa
    22021 tttaaaatat aggtaacacc tgagttgtgg caacaatcat taagcaaact aatatcattg
    22081 ctggcattaa gcgaaaagtt ggaatagttg tattgtcttt ttacgttttt cacaaattca
    22141 gatgttaact ctgaatttat tattttttgg ccaaatatgc ctaactgttg agtaaacgtt
    22201 ggtaaaatgg ggtatttcag taatcccatc ttgtttttta ccggaagggg catcacttgc
    22261 tcataattat cgctgacaag agcttcccag tccgggcata caatgtcaag ataccacgaa
    22321 agtgcatata tcaagccata aggagaagat tcaatggctt tatcccactg cgcacggtca
    22381 atttggtcgt ttgatagtag cttaatcaat tcttttcgga tggagaggtt tgataaaggt
    22441 tgttgagctt ttgtttgaaa gcctcgttgt tagccacatt gattaggcca atcagcccct
    22501 ggataatggt ggtacgttgc ataatctgca tcgggctcag tgagttttcg tccctgaaaa
    22561 ctatcttttg ggctttgtat cctgtaccgt ctaacgaaac tgcaacagca gcttcgtatt
    22621 tgtcaaatat caatacagaa ggcttgtttc ttcggttgta tttcgaaatg gcagtaatca
    22681 gcctgttttt gatataatca atttgttgtt gcccaaggta aggcgaaccc agcatcagtt
    22741 tgacaaggtc aatcttcgct agtgttttat aatccttttt tttgagcagc ttgttgtatt
    22801 ctctttgcga ggtatgaagc attagcttaa tgtatgcttg aggaatactg tttacatcgc
    22861 ccagtttgtt gtttttcttt cgggctttgt attcttcttt ggcatatttt tgttcaacag
    22921 gcaggtaaaa tctccatttg tgggtgcgtt tccaggtttc tgttacaggg ttaggcttcc
    22981 agttgctttc tggtcgttga agaattggat cagctatttc gagttggcgg ctttctaagg
    23041 caaataatat acagttgata aaatgtgccc aacctccacc taaagtttgg ccaacactgc
    23101 cccaaagggt tgcacggcga ggggctgttg aagatgcagt gtttaaggta taatccgatt
    23161 cctggttgtt taggtttagc aaactatcta tgtagctttg gtcaaagttg accgggtagt
    23221 agataaattc ggttttttta ttgaaaacct cgtcagggtc agccataatc aacttatcaa
    23281 agtcataatt ataggcagag tcattatcca tttttaggta aacattgtaa tcagccccaa
    23341 aagtcatttg ggtgatgttg atggtttgtt gctgtgcttt tgcaacaatg gtggctccta
    23401 ccagggccag gatgcaaaga aaacttttat tcatcggcca gttttgtagt tcatttatta
    23461 aagtgagtct caaatatcaa caataaagat attgagatat ttgagacttt atcttttgtc
    23521 ttacaatatt ttaagactaa ttctaaaaat tcttatgcgt ctatcttggc atattttgca
    23581 tgtttttcaa taaactcgcg gcgaggcggg acatcttcgc ccatcaacat ggaaaaaata
    23641 cggtctgctt cggctgcatt ttctactgta acctgcttta aaatacggtt ttcaggattc
    23701 atagtagtac tccacaattg ttctgcattc atttcgccca aacctttgta tcgttgtacg
    23761 tgaaccccgc ctttaccttt gccgatttct tcaatagctg ccattcgttg ttcttcggtc
    23821 cagcaatatt tttctatttt accttttttt accagatata gtggcggact tgcaatataa
    23881 agatgtcctt tttgaataag gtcgaacatg tacctgaaga agaatgtcat gattagtgta
    23941 gcaatgtggc ttccatcaac gtcagcatca gtcatgatta taattttatg atacctgagt
    24001 ttctcaaggt tcaatgcttt tgagtcgtct tcggttccaa tagttactcc aagagcagta
    24061 aagatattgc gtatttcttc gctttcaaaa attttgtgtt gcatagcctt ttcaacgttc
    24121 aggattttac ccctgagcgg aaggatagcc tgaaaacgtc tgttacgccc ttgtttagct
    24181 gtaccacctg ccgagtcgcc ctcaacaaag aatacttcgc atgtagcagg gtctttatcc
    24241 gagcagtctg ccagttttcc gggaagccct gagccagaaa gcacgttttt gcgttgaacc
    24301 atttcacggg ctttgcgggc agcgtggcgg gcagtagcag caaggataac cttattcaca
    24361 atttgcttag catcacgtgg attttcctca agatagttgg caagttctgt acttacagca
    24421 gcatcaacgg ctcccataac ttcattgttg ccaagctttg tttttgtttg tccttcaaac
    24481 tgaggttcag ctacctttat agaaattaca gccgaaaggc cttcgcggaa gtcgtcacct
    24541 gagatgtcaa atttaagttt tgaaagcata ccttcttttt cggcataggc tttaagggta
    24601 cgggtaagcc cccttctaaa acctgcaagg tgagtgcctc cttcaatggt attgatattg
    24661 tttacataag aaaaaacgtt ttcagtaaaa gaggtattgt aatgcaatgc tatttctact
    24721 ggtattccgt ttttttcggt atcgatatag atagattttt caattagttt ttccctgttg
    24781 gcatcgataa atgctacaaa ttcctgtaac ccggtttctg agtaaaaaat tgcctgacgt
    24841 gcttggccat tttcgtccaa ttcgcgttta tcaacaagtt taaggcgaat ccctttatta
    24901 agataagcca gttctcgcaa acgggcttct aaaacctcat aattatattc ggtagtaatg
    24961 gtaaatattg tttcgtcagg tttaaaagta atgatagtac ctgttttttc agtatctcca
    25021 acagtcttaa ccgggccaag gggctttcct ttactgtatt cctgtacata aatttttccg
    25081 tcacgatgga tttcggcttt cagataattt gataaagcat ttacacatga tacccctaca
    25141 ccatgcaaac ctccggatac tttgtatgag tctttgttaa attttcctcc ggcgtgaagg
    25201 atagtaagta ctacttccaa tgccgacttt tgttcttttt catgaaaatc aaccgggata
    25261 ccacgcccat catcggctac tgtaatagaa ttgtcctcat taataaaaac ttcaatattt
    25321 ttacaatgtc cggccaacgc ttcgtcaata gaattgtcaa ctacttcata aaccaaatga
    25381 tgaagccctc tggagcctgt atctccaatg tacatgcctg gccttttacg gactgcttca
    25441 agtccttcca atatctggat actatctgcg ctataacttg acgttgtttc tttgtctaaa
    25501 tcttcttgca tgtttgtatt tttgtgcttt gtaaattgtc ttttattttt ttaaacatac
    25561 aaagatacta tttccagccc aacattcaat acaaaaccat ttttagggaa ataatgtttt
    25621 caacaatttt tttgtctttt tgttagtttc tttagtaact tagtatctga ggtcttgttt
    25681 ttaggtacga attttgcatt ctttcggttt tatttgtaat gaaaggtttt aatctttatt
    25741 ttttaatttt taattgataa tcaatgttgt acaatcctca aaactataaa gtggcagata
    25801 aacccatgga gccatttata gatgagcagg aaatatggga ttttattgat agttcaaaac
    25861 ctgattcgca taaggtcaag gacataattg ccaaatcgct tgataaacat cggctaacac
    25921 ttgaagaaac agctacttta attaattgta ctgaaccagg gcttgtagag caaatcaaag
    25981 aaggggcaaa aacgttgaaa gaacgcattt atggcaaaag gatagtgctc tttgccccat
    26041 tatatgttgg caacttatgt attaataatt gcttgtattg tggctttcga gtttcaaata
    26101 ccatgcaaca ccgctctact ttaacgcatg acgagcttgt aaaccaggta atagctcttg
    26161 aagataaagg ccaaaagcgc ctgatattgg tttttggtga acatcctaaa tataatcctg
    26221 attttatcta cgatgcagta aaaaccgttt accaggtcaa aaaaggcaat ggcgaaatac
    26281 gcagggtgaa tattaatgct gcacctcttg atattgatgg tttcagaaga gtgaaagaag
    26341 ccgggattgg cacataccag atatttcagg aaacatatca tcgtggggct tatgcccaat
    26401 accacaaaaa aggtcctaaa cagcactacg actggcgtct tacagctttg gacagggcgc
    26461 aggaagcagg tattgatgat gtaggtattg gggcactggt aggtttgtac gattggcgtt
    26521 ttgaaacatt agggctaatc aggcatgtca accattttga agcggtatat aatgtagggc
    26581 cacacaccat atcgtttccc cggattcaaa acgcttcaca tttgaatatt ggtaaggatt
    26641 ataacgtaag caacgaggat tttaccaaaa tgattgctat ccttcgtttg gcagtgcctt
    26701 ataccggaat gatacttaca gcaagggaac cggcgtttgt gcgtgatgaa atactcgaat
    26761 ttggtgtaag tcagattgac ggtggtacca atatcgagat aaaaggatat actaaagatg
    26821 aagctgaagg acaaaacctt gacaaagagc agtttcagat taatgacaat cgcagcctga
    26881 atgaaattat caatgaattg gttgtaaaag ggatgattcc atcattctgt acttcgtgtt
    26941 atcgtgcggg gcgtactggc gagcatttta tggagttttc ggtaccaggt tttatcaaac
    27001 gtttttgtca acccaatgca atacttacat ttgccgagta tttacaggat tatgccccgg
    27061 aagacactaa aatagcaggg tatgctttga ttgaaaaaga gataaccgaa atggaggatg
    27121 cttcgctgaa gcaaaagctt gttgaaaaac tcgaagcggt gaagaccgga aaacgtgact
    27181 tatattttta aacaggtata aaaagaaaat gcagcgcatc gaagcgctgc atttaatgaa
    27241 tgatttagaa actaaaataa aaccccaaaa ctaaaaccct taaaaccttt aaaagcagaa
    27301 tgtctttgat aacattctcg gacgttgata caaagatata tttattaacc ttcagctgta
    27361 aatggtgaaa gtactaaaat caatttgtaa aaacactcaa ttttatatga gtgaaaatac
    27421 ttatataaaa gttctaattc gcacaaaatc agattaatag tagctttcaa tgaatatttg
    27481 acttaactga aaacctttct tttttaagat ttctatggca tcatttatca tggccgaact
    27541 gccgcaaaga tatacttttt ggtccaaatc aattttggaa tcatgcaaat attgggtcaa
    27601 cctgccatga taaccgccag agttgtcacc tgttgtacaa atggtaattt tatctttagg
    27661 gtaatgctcc ttttcatagg catcttcaat atacctgaca ccatgaataa tatgataatt
    27721 caagtctggg aagctttgtg taaaactgtg aaaaggggaa atacctgtgc ctgaggcaag
    27781 aaataagtat ttcgttttgt ttatgtcttc ttggtcaagg caaaaatgac ctgaggggcc
    27841 ttcaatatga acataatcgt ttattttaag atgttttagt ttgggagtaa gcacgccatc
    27901 ttcaacttct tttaccaata cttcaaaata tctgctattg gtgccactat aaatggtgta
    27961 aaccctaaag cttgaatatt gcggcaactt gatcctgata tgctgccctg ctttaaacgt
    28021 tttgttttta cgctcaaagc taataataaa ggcatccttt gtgagatgtt taatggctgt
    28081 tactttgtgg tatttttcaa tatccttttg ctgaattttt aaattttcct gaaaagtgat
    28141 ttctggtgaa actgaatgca ttaacatatt ttcgatgagt ttgatttata ttacacaaac
    28201 acataaaaca aaaaagtgtt gaatttgcat ccaacacttt ctgttcattg gtctttattg
    28261 gttaaataag gtaaaaatga ttaatggtga tgttggtgcc ccgaaggttt tgcttttttc
    28321 ttttttgaat gaaaaagtga ttttttcagt ttggccaacc atgatatttc gtcagtaaaa
    28381 ggcaatgtaa gctcaaaggt gaccggatgg aaactctttg tacgtctgga agagtccacc
    28441 tgcactactt taagcagcat tttacctgag ttgaaatcga tgttttcttc tgagaagcca
    28501 acagctgtaa gtttaccgcc aatgtccacg cctgtgtcga tacaaagggc tgcaacagct
    28561 cctcctcccg gaaaatagac tggattggga atatctgaca tgtcgtagtt gtaatccttg
    28621 tccttgctgg catattgaag acggtttttg ttttgcctgt attgggtgcg gaaatgccca
    28681 tgtaccagca ggtatccgtc ccataccgat aagctttttt gcaaaagcct gtcgtcattg
    28741 tttaggcaat tttcttcaat agctatattt ttagaattta tgtaattacg aaagtctttg
    28801 taatgttgta ttttcaattg ttcttctaac ggaaaatcta aaaaaggacc ggcatgcgaa
    28861 aaaagcaggt tcagttgttt tttgccagaa cctaaggttt cagtatgaaa tagttgcaga
    28921 ttgttaataa attcaagatg atgcttatct atttgggctt taagactttc tacatcatgg
    28981 gcttcataag ctttcaaagt gttttcgtag ccgtgtttgc tccactctaa tactgctttt
    29041 gggttagaag cttcaccttc aatggcattc aaaagcagaa actcgtgttc gcccaataaa
    29101 gtaacctttt cttctttaat atttgttatt gtgttaataa catcttttgg ttcagggcca
    29161 cggtctatat aatccccgac aaatatgagc tttttgttgt tgtatgacga gatagcattt
    29221 aacacggcct gaagtgactg attgtaaccg tggatatctc ctatagccca tatatattct
    29281 gactttgcca tattgctatt ttttttaaaa cataaagtta atgatttttt tatcctcaaa
    29341 ttgaatttaa aattattatt tagtacttct ttgagagata ttgtagtgca aaatttgtag
    29401 aagcaacaag gcttttgtgt atctttgttt ttttataggt tacagcttgg aaaatgatgt
    29461 tttttataca ataaaaaggc ctgctgaggg tgtttttaaa gataagggga gtaaatttct
    29521 ggcttttgct tttccaattg aaagtgagca ggagtttaaa gaccatttag cgaggttgaa
    29581 aaagcagtat tttgatgcac gccatcattg ttatgcattt gcaattggca tcaatcgcga
    29641 gattaaccgt tctggcgatg atggcgaacc ttctgggact gctgggaagc cgatttataa
    29701 ccagatactt tcgtcaaacc ttaccaatat tgccattatc gtggtccgtt attttggagg
    29761 aaccttgctt ggtaccagtg ggcttataca ggcgtacaaa ggggctgctg ccgatgctat
    29821 agccaatgcc gaagtgattg aaaaacatgt tcaggctaag ttaaatttga gttttgaata
    29881 tgaattactt aaccaggtat tgaaaatagt aaaaggcgaa ggcgctgata tccttaatca
    29941 gcagtatgac aatacttgta cgcttgaggt ttcaatcaga gcttcaaaat tagaactttt
    30001 gagtaataaa ttggagaaaa tcccgggagt aagaattgaa cattaatctc agtttgattt
    30061 tttggagata agatggtaat tttatatttc tgctgacata aggttgtcac ttcacaaaca
    30121 tagctttgtt gtgttaaact taaaaactac taaaatgaaa aaagcattga tcattgttgg
    30181 ctcaataatc gggttgcttg tcctggttgt tgttatttat cttgctgtta tggggatgtt
    30241 ctcaagtccc aaggttacag aaaaagagat ggggccttac tgtttcgtgt atgaagattt
    30301 taccgggcca tatcagaata ccggacctgt ttttgataag gtttacaagg caatgacaga
    30361 tgctggcata aaaagcacgc ttgggatagg cgtgtatttt gacaatcctg ctgaagtccc
    30421 ggctgaaaag cttcgtagcc aatgtggcgc tatttatgat attgcagatt cggccaaagt
    30481 ggctaaactg ttgcctactt ataagttagg aaagattgag aaaaagacgt ttgttgtggt
    30541 tgagtttcca ataaaaggag caatgtcata tatgattggc ccggcgaaat gttatccggt
    30601 gctggataaa tactttacag aaaaaggcat gaaggctgcc gaatcttttg aaatatatga
    30661 tatgcctaat aaagtaactt attatatcat ggcaggccgg taattttagg ttttctgcaa
    30721 ttaaaaactg cataagggat tgttacgcaa agcaatccct ttttgtttgc ttctttgtta
    30781 tgcatgtttt caggtttttc aaaaattggt tcgcggcctt tttcttgtag gacagaatac
    30841 caggactcca taaatgccag cataataaag tattgtttaa catggcagtt ctttcttttt
    30901 atttccaaac aaaagagtaa accaattgga tttttgggat taggtttttt attgctgaag
    30961 caaaaatgtt tactgaatgt ttattaagct tttttaatgt gtaattaaga tttaaaaacg
    31021 taaatttacc aatagttgga aataggcatg gggcagttac gaaaatataa aatgcaaata
    31081 ggtatcatac tttcattagt gtttttaatg gaagcgatgc ctttatgcct gaaatctatt
    31141 caacagcctg ataatactta caatacaata gttaaaacgt tttcaggggc agcttcaaac
    31201 cagtatttta gaccttccaa agcaactaaa gcagctgtat tttttaaaca attcttttat
    31261 gaaaaaacat attttgaaag tgttttttct tccattattg cagcaaatat ggttaagata
    31321 tattgctgta ccccaatata ttcaaatgtt tcattgttga aatttatttg catttttaaa
    31381 atctgattta ttttattttt taacaatcaa tggaaaatgt tcgtagccat taaatggttg
    31441 acggataatt ggttaaacgt ataataaaag taatcatggg tataaatctt gagaaaataa
    31501 atgaaaacac tgagcccacg gatgataaaa ccaattgggt ttatattata gttggcataa
    31561 tcattttgat tactttgctt ttattgacca actgagcctt tgttttagta tgagcagata
    31621 ctgtttatcg gcctgctcta agcctttatt gataattgac taaaaaaata gaacttactg
    31681 atggagcatt taccaaaatt aattgaagat cttgccctga taatgattgt aggggcttta
    31741 gtaaccttat tgtttaaaaa aatcaaacaa ccattggtgt tagggtatat tatagctggt
    31801 ttttttgttg ggcctcattt atcgttagtt ccgacagttg ccgatacaga aaacataaaa
    31861 acgcttgcag aaattggggt catctttttg ttgttcagct taggcttaga gtttaatctg
    31921 aagaaattgc tgcatattgg aggagcttcg tctattaccg ccttggtaga aatagttttt
    31981 atcacgctga cagggtatat gttgggaaag tttatgggat ggactacaat ggacagcata
    32041 ttcctgggag ggatgttggc aagctcttca actaccatta ttataaaggc ttttgatgat
    32101 tttgggatta aaaccaggca atttgctcat gtggtatttg gggttttaat agtcgaagat
    32161 attgtggtaa tacttttatt ggttttgttg cccaccatat cggtggctca gcaatttgca
    32221 ggggttgata tgctgtacat agtactgaaa ttgctgcttt ttcttgttat gatgtttgtt
    32281 tcgggcatat ttatattgcc tacattgatg aagcaaatga ggaaatttct gaatgaagaa
    32341 actttgctgg tattgtcagt cgggctttgt ttgggaatgg ttgttttggc tacccgcatt
    32401 ggcttttctg ctgagttagg ggcttttatg atgggtttta tcctggccga gacatcttct
    32461 gccgagaaag tagaacaggt tataaaaccg gtaaaagatt tgtttgctgc agtatttttt
    32521 gtttctatag gaatgatgat agatcctgca ataatcattg aatatggttg ggcagtgata
    32581 gccgttacgt tattgactat atttgggaag ctcatcagca ctactgccgg ggctatactt
    32641 tctggccaat cgctcaaaca atcggtacag gtgggtatga gtatgtctca aataggtgag
    32701 tttgccttta ttgtggcaac gttagggctt tcattaggcg tgataagtga gtttttgttt
    32761 ccgatagctg ttggggcatc ggcaataact acgtttacaa cgccttacat gattaaatat
    32821 tcagacaaat tttatggttt cttagaaaga agcttacctg caaaatttct tgagtcagta
    32881 aataactatt cgcttagtac tcaatcaatt aagtcggaga gcagatggaa atctgtattt
    32941 aaatcttata tgtttattgt gctttccaat agtattattg ctttttcgtt attatttctg
    33001 ttggttgaat ttttatttcc atttttaaaa gaggttttcc ctcatcaggt aatatttgga
    33061 gttgtatcac ttattgtttc acttggcgtt atttcgccgt ttttgtgggg aataatggca
    33121 aaaaggcctg gcgatatagc atacaaagaa atgtgggtca atgaaaagta taataggggc
    33181 ctttgttgat attagaaata tttagggtaa ttattgggct tttaatcgtt ggttttttgg
    33241 tcaatgctgt ttttacaacg gttgtagcat tattaatatt ggttccgttt atccttgtgg
    33301 ttttatttat tttttctaat catattaaga aattttatca gcgtttagaa gataggtttt
    33361 tgaacaacct gaatacgcaa aaaagccatc aggacaagga tgatgcttca atcaatacac
    33421 aaaagcaaat ttcgcaatac cttgaccaga ctccctggaa tatccatatt atagagcttg
    33481 aggtggggcc acatgctacg tttataggac aaacgcttga aagtctttca tggagggaga
    33541 aatttgggat taatgttgct tttatcaagc ggggcgagga aatgatttat gcaccatcac
    33601 gtataagcag gcttttcccg tttgataaaa ttggtattat agctacagat gaacaaatgc
    33661 tggctttttc tgctgttttc aacgaagtgg ataaaataaa ctgtgttaaa cacaaaattg
    33721 aagatgtagg tatttgtgca gttcagataa atgaatataa taaacttaaa ggcctttcaa
    33781 taaaggattc tcggatacgt gaatatacca atggcatggt aatagtgatt gaaagaaata
    33841 aacaaaggct actgaaccct gtttcggcta cagtatttga gtggggagat attgtttgga
    33901 tagccggaga aagaagtaaa attcaaaaat taaatcaaag ataaagttgg gtaatacttc
    33961 caaaatcaac ttttatttca aatgatttgt ttgggtcgag gccggtaagc aggcatgata
    34021 aagctctgat aaccccgcta tgggttacga ccaggagggt ttcatttgtg tgggcttgct
    34081 ttaagaatgt ggtaaactct tttacccgct ggagcaattg cctgtatgac tcgccattgg
    34141 ggcaaggctg gttaatccaa tccttgaacc agttattagc ttcagtggtt ggtgtgattt
    34201 catcccaggt tttaccttcc caactgccaa agtttagttc caacagtctt gaatcatagg
    34261 taacaggtgt tccgttggct atatcttcgg ccaattttct gcacctgagc gaaggacttg
    34321 aataaaccgc ctgaaaagac tcgtttttca acttactaac aatttcattc ttttctgtat
    34381 cgtatgaatt ggctgtatct acatctgttt gcccatagca aagcccggct gggacgttaa
    34441 cagtagtatg gcgtatggca atgagtttca tagttatagt ccacctaact tcacttataa
    34501 aggggaggaa atagaaacag cttgttttgc aataatagct cctacataaa cggcaatctc
    34561 ggttagttgc tgtaaagctc ccagtacatc acctgtgtat cctcctaatt tgcgggttac
    34621 ataccatctg aagtagataa atagcggcaa agacaatgct aatattacaa gtgcttcttt
    34681 ccatgagata aagaaaaaac ctgcagtgcc aaatataccg gcaacaatga atgtagttat
    34741 tgaacctttg tgccctattg gttttgactt gcttagagca tctttgcgta catattcaga
    34801 tgtataaata aggcatacag gcataaagcg actgaatgca tgagcagtga caagcattat
    34861 tggaatttca aatatagaaa agcttgaaat acataagaat ttggaaagta acataaagat
    34921 taaccctaca gctccataag ttccaatatg gctatctttc ataatctcca ggatgcggtc
    34981 gctggtgtag cctccaccaa agccatcgca aaagtcagca aaagcatcct cgtgaaaagc
    35041 cccggtgagc agtactgtag tggccatgct aaagattata gccaatgtca atggcagaaa
    35101 catatacagt gtccaaaata ccaaagcccc ggtacctcca acaataagcc cgataaatgg
    35161 gaaataacgc gttgcggcat ttagcatttc atcagaaaat gccatatttt ttggcacctt
    35221 aattcgggtg taaaacatga tggcggttag gaggatatga agctctcgtt ttatcatatt
    35281 actctttatt tgaaactccg gcactttcaa agctggccat ttcgttcaga aacaatacgg
    35341 ccgattttat caacggatag gtaacagcag ctccggtacc ttcgccaagg cgcatgccca
    35401 agtccaccaa tgcaacgcct ccaaggtagt cgagcatagc tttgtgcccc ttttctcctg
    35461 agcaatgaca gaagatacag ttgtccagaa tggttttatc catattgtaa gcggcaagca
    35521 aagcagatgt cacgataaat ccatcaacaa tgataatcat gccattggct ttagcttgca
    35581 gcatggctcc acacatcatg gctatctcga aacctccgta cgtagctaat atttctaacg
    35641 ggcttgaaac attatgcttg tttgaagctg ctttcagaat ggcaattttt cgctttaagc
    35701 cttcatcgtc atgtccggca ccttttcctg tgcaatcttc aatggcgatg cccgtatatt
    35761 tgttcatcag cagtgaggca gaagatgtat tgccaatacc catttcgcca aagctgatga
    35821 tgttgcaacc tttcttaaat tctgcattta cgatgtctct gcctttatcc aatgtttttt
    35881 ggcagagttc gacagtcatg gcaggttctt tcatgatgtt tttcgttccc ctgcccatct
    35941 tggcatgtac gatattggct ccctgaggaa attcaaagtc cacaccggca tctaccactt
    36001 taaggtcaat accattttgg cggcaaaaaa cattgatagc tgcacctccc gaaagaaaat
    36061 tcattaccat ctggtaggtt acctctttag ggtaggggct tacgccttcg tctgccaggc
    36121 catgatcggc agcaaagaca atatgtgtag gattcttcaa ttctggagac agcgtgtttt
    36181 gtatcatccc aattttgaca gctatatctt caagcttacc aagtgaacca agaggcttgg
    36241 ttttaaaatc aactttgtgt tgtaatgcgt ttttcataat aagataaata gttctatttc
    36301 attttttttg ttaaaacccc tcctaacctc cccttataaa ggggaggaat cagacgaaac
    36361 aatgtcagtg ctgtatttcc tcccgcctga ggcgggaagg ggtaggggtg gttttgattt
    36421 ttaccggaat tcctgatacc atcagatata cagcatttgc taatgaagca atgtattggt
    36481 tcatccagcc ttgaaggtca gcaaactttc gtgcagattc atgttcgggg ataatcccca
    36541 taccaatctc attgcttaca acgattaacg tgaagttctg ttggataaac tttgtccatt
    36601 cttcttttgc ttgtgttaat gcttcttcaa ccttatattc attgtcatgg aaaatattgg
    36661 taagccaaag cgttatacaa tcaagcaaca cagttttgcc atgtaaatca agcccagata
    36721 tatgcttttc ttcttctatg gttgaccatt gattgcctct atctgcctga tgtcgtttga
    36781 ttcgttcttt aaaatcggca tcccaagcgc gggctgtagc caaatatacc gggttggcag
    36841 atttttgctc tgctaattgt tgagcaaatc gacttttgcc agaacgctgt ccaccagtaa
    36901 tgaaaatgat ttcagccata ctgaattatt ctttcatttt gtttaaggtc ttttgcgaaa
    36961 ccggaaatat ggaaggtttt cctgacaaag ggtttatttt ggttataacc acggtattat
    37021 aaactttttc aatattttca taagtcaata cttcttcggg agagcctttt tgctgaattt
    37081 cccctttgtt gagcaggata agctggtcgc agtattcgcc tgcaaggttt agttcgtgga
    37141 tgatcataag cactgtaagc cctaactgtt cgttaagttg ctgaacaagg ttaaaaacct
    37201 gtatttgatg cgtgatgtcg agatgcgaag taggttcatc aagcaatagc agttctggtt
    37261 cttgtacaag tgcgcgagtg atagctacca tctgtctttc gcctccgcta agctgcgtca
    37321 ggtacttgtt acggaggtga gcaatatcag taaggttcat atatttctcg gcaatatcgt
    37381 aatcgtcttt tgtatcgaag aactggtagc ttttgtggta agggatgcgc cccatcaata
    37441 cgtagtcttc tactgttatg tagggcgttt caatgtcttg tgtcactatg gctatgttgc
    37501 gggctttttc tttgtgcgaa agatgtttca ggttgattcc attaagcttt attttcccgc
    37561 ttttgagctt cagttctccg gtaatacctt tgaaaagtgt agtctttccg cagccattgg
    37621 ggcctatgat acctacaaag cttcctttgg taatgccaat attaatattg gagatatgaa
    37681 atttgtcgtc atatccgcaa cttaggtttt ctatttcaaa aaaataagcc ataggctaac
    37741 ttagttttgc tttagtagaa ctgcggctta gcattacaat gaaaactaaa cctcctataa
    37801 tacctgtgat tacaccaatg ggcaattcgt tgggagcaat aatggtacgg gcaacagtat
    37861 cgcagaagac aagaaaactg ccgccagcca gaaatgagct gataagcagg atgcggaaat
    37921 ctgaacctgt taaaagacgc atcaattgag gtatgataag gcctacaaag ccaatcacac
    37981 ctgcaacagc cacacacatg cctgtcaata aagatgccag gataaacaat attttaatag
    38041 ccacatcagt attgataccc aggtggcgtg ctttatcttc acccaggcgc aaagcattca
    38101 gcggatggat aaacaggtat gatacggcta acccggcaac agacatggtg acaccaagag
    38161 caatcaggct gctattgggc tcgtcaagcg accccataat ccaaaacaca atgctttgga
    38221 tgttgtcgct ggtggtgtat gacatgagca gcatcatgcc tgatgaagca ataaagctta
    38281 tcattacgcc caccagtacc atgttttggg tcaatacttt gccgtttcgt acactcagaa
    38341 aatataccaa aaagatggtg gccaatgcgc ctataaagcc tgcaagtggc aatagaaaag
    38401 ccccaactct caggctaagg cctgtaacta ttataaaagc aacacttagc gcagcacctc
    38461 ctgatatgcc cattgtgtat ggctcaacaa gcgggttgcg gtaaacaccc tggagtatag
    38521 ccccggcaat gctcaaagcc ccacctactg aaaggcctat atatatcctc ggcaggcgaa
    38581 tgtctctcag aacggtatat tccatgcttc cttttccgtt aaacagtatt cgcgggatgt
    38641 ctgtcaatga aatatgcaac tcgccaatgg aaagcgatat cagtacagac aatattgtaa
    38701 gtagcaggaa cgctaatatc caaagccccc atttgatgtt tttggaaatg ctcatggata
    38761 catagttttt ataagttgtt caacaacatc tacaaaacta actgggtcgg ggctacatgc
    38821 tttgtctgaa tcaacaatga agattttgtg ttttttcaca gcgctaatat tgctgtattt
    38881 tagccatgca gctttttctt catcgcccac aacgcccatg gttacaataa aaataaaatc
    38941 agggtttcgc aaaaccacag cttcgcgggt tatagtgcct tgtttgaggt cagcagcaat
    39001 gttttgtccg ccagagtatg tgataaagtc atccatgaaa gtattgggaa taaccgtaaa
    39061 tagcggtttt gccccaattt cataaaatac tttgggcttt ttatcctttg ggatcaatga
    39121 ttttaaccgg tccaacctgg cttgttgctg gtcaataatg cttttggcct tgctttgctt
    39181 gttggtaagc ctggccagtt ctgtaagttg ctcgcagatt tgagtgtatg attttggcga
    39241 aggcatgacc tttactttga ggccaatctt tttcaaagca gcaatatctg taggttttgt
    39301 caggtcagtt gttatcaccc aatcaggttt taaggtatat atcttttcgg tgttgatttc
    39361 catcccggta attacaatgc tttttttatc cttaatagct tctatacaat agttggtaca
    39421 gcctacaagt ttttcttcgg cttcaagaat atacagcatc ttggttagcg aaggtgctaa
    39481 tgaaacaata cgctgcgaaa atgtatttaa gcttagtatg gcaaatacga aaaacagaat
    39541 atacccgggt ttcaatttca tgatttcaac atttattgaa ttatcaaagt aagtatttgt
    39601 acgttacttg agaactatga ggaatgtttt ttttataaaa aagcaaagac acaaagtgca
    39661 aagaacaaaa tctaattgag tttctttgtg tcgttgtgtc tttgcgtttt aaaatcttta
    39721 ttaataacta aaaactacag aatttgggaa gcttgcagtg ccaaagctaa atag
//
LOCUS       DAOSPF010000031        39139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1076_length_39139_cov_6.598378, whole genome shotgun sequence.
ACCESSION   DAOSPF010000031 DAOSPF010000000
VERSION     DAOSPF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 39139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_39139_cov_6.598378"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PK252_07815"
     CDS             <1..1159
                     /locus_tag="PK252_07815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT14654.1"
                     /translation="NNLQVCIMAPTEILAAQHFKSISRMIDGLDINIRLLTGSTRKKE
                     RTIIQQGLTDGSLQILVGTHALIEDNVQFKKLGLAIIDEQHRFGVAQRAKLRNKSAQP
                     PHVLVMTATPIPRTLTMTAYGDLDVSVIDELPPGRKPIYTKHIGINHRMDMYRLVARE
                     IQSGRQVYIVYPLIKESENLDYKALEKGYEQMVKIFPAPQYSISAIHGKMSNEDKESI
                     MKAFKEGKINILMATTVIEVGVDVPNASVMIIESAERFGLSQLHQLRGRVGRGADKSY
                     CFLCTSFKLSTDARKRLEIMESTNNGFTIAEADWKLRGPGNIEGTEQSGMPFSLHIAN
                     LGKDQQILQYARDLAQKIIDDDPHLQKSENVKLNILVSKTKKEQYNWELVG"
     gene            1196..1927
                     /gene="gldF"
                     /locus_tag="PK252_07820"
     CDS             1196..1927
                     /gene="gldF"
                     /locus_tag="PK252_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOT14655.1"
                     /translation="MISLFRKEISLFFNTLTGYLVIGVYLVLNTLFLWVIPGSFNIPE
                     SNQASLHGLFTISPWLFLFLVPAITMRMIADERKSGTIDLLFIKPISDIQIIAAKYLA
                     SLVLVAAAIIPTFVTFFTVYHLGYTPGNMDVGATIGSYMGLFFLAAIYAAIGVFASSI
                     TENSIVSFIIAALLSLLLYIGFDTLSQLPIFSGIEDAIKSIGINEHYNSISRGVIDTR
                     DLIYFAGVSAIFILLATYSIEKKHQ"
     gene            2110..3798
                     /locus_tag="PK252_07825"
     CDS             2110..3798
                     /locus_tag="PK252_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14656.1"
                     /translation="MRKLLLITSMLCYMAGAWSQDVASKKVVNRDNNPSKKYLRPSIT
                     VLFAKRNNDRSARMEKVFQAAKTQDKYNNHNVSTFAIEPSNDYSKYLSENVSRQIVAK
                     WFNRNEKGQFDMNLIAERGFYNASDADVLKANASVRKDALLADAGENLLDRSYILIYD
                     IDPILTMDEYYKRIGTPNNLMEGYAVQYKVSVYKLDWTDSVAAVFYNMWTDASSPDPN
                     KVAAFDKTNFPVKYVGQVSTAFGWVTSSQFKDHSKNIFGSYSDDELFARIYPLIEKET
                     SISLPKLNEDFRVKTPVYSTHPIAAKIGLKEGLSVDKRFFVYEYELTNSGEKKAVRKA
                     VIRATSRIADNRKVATGRSDSSVFYQVAGRKVYEGMQLQENPDWGIGLTVGYGSTSGA
                     IGEGLTGILEMNASLWAGNVFKGLPPGIKIYAQGSLATDEFTVENGILDKKYNITSYA
                     FGISKDLNFLHHFVLVPFVGYGQEQLVNTETDDEKWKNDKYDGTFLHFGARFAMNLNY
                     NFQIIACYSLNSYLSLEPNTDNPNKESQIVKDWAEELKKGRGLGQLTIGLRYQF"
     gene            3867..4388
                     /locus_tag="PK252_07830"
     CDS             3867..4388
                     /locus_tag="PK252_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14657.1"
                     /translation="MKNRILTVTLGLAFLLTGVVSTFGQARKKAMRDMEDYRYELECV
                     GVGAQGTYLIKVWTYSKKPKVAIAQAKKNAIHGVIFKGFPGGGQGCTSQQPFATPADE
                     DKNIDFFEKFFADGGDYMRYVAESSDGSIGAEDRLKVGKEYKIGIVVSVQKDLLRKDL
                     EKAGIIRALDSGF"
     gene            4603..5550
                     /locus_tag="PK252_07835"
     CDS             4603..5550
                     /locus_tag="PK252_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOT14658.1"
                     /translation="MMKKIIISLAAIMMCVASFGQAKKPTLMVVPSDNWCKQNGFTMK
                     FDNQGTIEELPDYKKALQGSSDLMLVTAKISELMANRGFPCKLLEQEMKKLASESAED
                     AMLTSKQGGGVSESPIDKLAKTAKADIIIQVTWAVNTQGPKKSVTFILQGIDAYTGKQ
                     VASASGTGAPSFSAELPKLLEEAVLNYIDNYNVQLQNHFDDMFKNGREIIIRIKKFDT
                     WADDFETEVDGKEISAHIEEWMQKNTVSGRFNTSDATANMMLFEQVRIPMYDANGKAL
                     DARSFCKGLQTFFKNPPFNVTSKLMTKGLGQATIVVGDK"
     gene            5623..6588
                     /locus_tag="PK252_07840"
     CDS             5623..6588
                     /locus_tag="PK252_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14659.1"
                     /translation="MKKLSIILLSVFAFTVMAKAQNNEAKADDYARIALHSAVPNIAD
                     MPEAAKKLLKNKLDQVATNTGAGGNAKNPRFIITANVSVLTKDITATAPPMTAITLDI
                     TIFIGDAQTMTKYSSASIQAKGVGTNETKAYIEAIKQIRANSPELKNAVETGKRKIIE
                     YYNSQCDFIIKKAETLASQSKFEEAIFELSSVPDVCKDCYNKSMNAVGPIYKKYIDKA
                     CKEKLNAAKGVWAGAQNWDGAQQVSQILGTVDPEAACINEVQDFIAEVKQKIKEMDQR
                     DWDFKMKVFDTSVELEKQRIEAARQVGIAFGENQPDTEVNIVGWK"
     gene            complement(6696..7238)
                     /locus_tag="PK252_07845"
     CDS             complement(6696..7238)
                     /locus_tag="PK252_07845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOT14660.1"
                     /translation="MSNALIIIDIQNDYFENGANPLKGSYEASLNAAKVLEHFRENHW
                     PVIHIRHISKRPGSTFFLPDTVGSEIHKHVFPLKDEKVVIKHYPNSFRDTELLKLLQE
                     RNVTNLTVCGMMTQMCVDATVRAAKDFGFEVTLIADACATKDLTIQNKSITAENVQNS
                     FLAALNYFYAKVVSTNEYLS"
     gene            7743..8399
                     /locus_tag="PK252_07850"
     CDS             7743..8399
                     /locus_tag="PK252_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT14661.1"
                     /translation="MIKKKYRKTVLYSLLASALIIIIISVGLLIYFKNSNKPKRLLPP
                     KVVAAELTRLNIAIPEMKEEPKQVLKKAKEKIKGQPVEKSEEPVVPVETAKVEKSDSA
                     KLAEKESNLEAQDPRLKEEGVFQCNNTQDFRLWFINHFTFPLDEGVKKSEGRITLDFM
                     VGLNGLVDTVIIISGIDPIVDSEVKAVIYNSPRWKTCPIDGKPVKQKYRFTVYLVRRM
                     "
     gene            8420..10624
                     /locus_tag="PK252_07855"
     CDS             8420..10624
                     /locus_tag="PK252_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT14662.1"
                     /translation="MKVSNFFRVEGMSCAACASSVESIVGAVEGVSLASVNFAANTLM
                     VEYDDEKTSLDIIVSAAGNIGYTLIEDIGISIEQEEKKQEQKLRSMRYRLIGSFVFAV
                     PVFILSMFLHHWHYSVYLQSILSLPVVLWFGKGFYIQGFKKIFTRYANMDTLVAVGTG
                     SAFIFSILNLVFSGYIINKGFEPHVYFESAVIIISFVLLGRYIEELSRNKASESIKGL
                     LLLHSKTAIVIKDGQDVEVSADTIQTGDMVRVLPGARVPVDGIIVSGQTSIDESMLTG
                     ESMPVDKTAGDKVIGATVNLTGSITVEALRVGSATMLGNIIRQVREAQGSKAKVQHMA
                     DKIASVFVPTVFAISLLTFAIWFFAAQDNNVFRAFQAFFTVLIIACPCALGLATPTAI
                     VVAVGKASSHGVFVKDAALFDKAGKIDAIAIDKTGTLTEGKPVVIDFIDYSGKENGLS
                     AIFAVEKHSEHPVSKAICHFLNNRNNESLVVEDFEAVPGKGIKAKVNNAWHFIGNMSF
                     MAENGVVISEPHKIAIDALDDKARTVVIAANMNNLVAIFGIADTVKSNAAEAIRLLQH
                     EGVEVHLLSGDDKKVVAAVAGQLGIDQYKSRMLPADKASYIANLQQNGNCVAMAGDGI
                     NDAPALSKADISFAMSNGTDIAAYSAMATLMHGDITKISSIISYSRKTMRIIKENLFW
                     AYFYNVLAIPVAAGILYPIWDIQLNPMIAGAAMAFSSVTVVLNSLRLRWYRF"
     gene            complement(10692..12014)
                     /locus_tag="PK252_07860"
     CDS             complement(10692..12014)
                     /locus_tag="PK252_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT14663.1"
                     /translation="MEPTGEIQILVNELNNRLMYVLRHVFSKMLGLRPIITNDEESFL
                     KFQGFKLNYSRTKYADELYIHPAGLVYKKGIQPVYAGVDFINEMPVLFKAETEDGCDA
                     GFDIFSSIFFVISRYEEYLPFEKDHHNRFRPECSVLFKHELYRKPLVDIWTEELKRIL
                     KNRYQDITFTVHHFQNQPTIDIDNAFAYKYRGVFRTFFAFVRDILTLRIYTFQDRLRT
                     LMRMKADPYDTFVFLRTCLYNCHPKPYFFFLSGKRSKYDTNISLKQGMMAKVISNVAE
                     YAHIGLHASYASSKKKRDYLIGKELTMLENVSNRKVSANRQHYIKLHLPDTYQHLIKY
                     GIKEDFSMGYASDYGFRAGTCHPFMFYDLQKEKETPLLLVPFVAMDATFSTYLKYTPE
                     QAQEIIDNLKQTVEKYQGTLCIIWHNETFAPTKAGMAWRKVFERLYNG"
     gene            12133..12807
                     /gene="trmD"
                     /locus_tag="PK252_07865"
     CDS             12133..12807
                     /gene="trmD"
                     /locus_tag="PK252_07865"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT14664.1"
                     /translation="MRIDILTVVPGLLHGPLDHSIVQKARKKGLVDIHIHNIRDYSND
                     KHRKVDDYAFSGGAGMVMTIEPIDNAISALKQEREYDEIIYTSPDGEKFNQKMANMLS
                     TKNNILILCGHYKGIDQRVRDHLITREVSIGDYVLTGGELAAAVMTDAIVRLLPGAIG
                     DEQSALSDSFQDNLLAPPVYTRPAEYKGWKVPDILLSGNTKLIDEWNLQQSIERTKKL
                     RPDLLE"
     gene            12928..13194
                     /locus_tag="PK252_07870"
     CDS             12928..13194
                     /locus_tag="PK252_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14665.1"
                     /translation="MTTLELKTKFHKLIDNINNDDVLSKFYEMLSKANEVKKGSLWNR
                     LTDEEQQELILLEKESHNPDNLTAHAVIKKNIKNGFKTIWELVN"
     gene            complement(13265..15844)
                     /gene="mutS"
                     /locus_tag="PK252_07875"
     CDS             complement(13265..15844)
                     /gene="mutS"
                     /locus_tag="PK252_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT14666.1"
                     /translation="MRQYYSFKSKHPDAILLFRVGDFYETFGEDAIKTSEILGITLTR
                     RANGAASFVELAGFPHHAIETYLHKLVRAGQRVAVCEQLEDPKLTKKIVKRGITEMIT
                     PGITYNDNVLERKENNFIACLHFEKTLTGIAFLDISTGEFLVAQGTNEYVDKLLSNFQ
                     PKEVLFEKSKTKDFVDNFGNKFYTYKLDDWVFQYDNCHDKLLKHFNTTSLKGFGIHEI
                     TAGVVSAGAILQYLELNEQNQISQITSISRIEEEKYMWLDKFTIRNLELVRSMNEDGK
                     SLLDVIDKTLSPMGARMLRRWLMLPLKDVDSINRRLNIVEYFISQSDIRDIAAEHIKV
                     IGDLERLASKIAVSRINPREVIQLKNALEAIIPLQQICAESGMPEMVRISEQLNPCRS
                     VIERISKSLVEEPPVQIGKGTIFNPGINEELDELKSIQTKGKDYLLQLQQREAERTGI
                     PSLKISFNNVFGYFIEVRNTHKDKVPADWIRKQTLVSAERYITQELKTYEEKILGAEE
                     RITVLESQLFEELIHFLSEYVQAFQLNANLIGQLDCLLAFAFVSAENGYSKPVVNDSD
                     VLEIKEGRHPVIEKQLPPGNSYVANDVLLDTVEQQIMIITGPNMSGKSALLRQTALIV
                     ILAQMGCYVPAKAANIGIIDKVFTRVGASDNISSGESTFMVEMIETASILNNLSARSL
                     VLLDEIGRGTSTYDGISIAWAIAEYIHEHPTAKSKTLFATHYHEMNEMENSFKRIKNY
                     NVSIKELNNNIIFLRKLVRGGSEHSFGIHVAQMAGMPKSVVKRAGEILKELEDSQRSD
                     KPQRPAPETTAKREGYQLSFFQLEDPVLKQIRDQIKNLDINTLTPLEALNKLNYIKRI
                     TGL"
     gene            complement(16012..16677)
                     /locus_tag="PK252_07880"
     CDS             complement(16012..16677)
                     /locus_tag="PK252_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT14667.1"
                     /translation="MYIDFHTHYCNEDANVLAIENITFFRDKINSINPQKFYSVGIHP
                     WYIRDDFSISEQMKQVKELAINLNILCIGETGLDKIRGAEWSLQLEVFKKHIELSEML
                     GKPLVIHCVKAFDVIFQLKKQIKPQQKWIIHGFQGKAVLYKQVIDNGLMVSISKKFFN
                     AEFIEMLDKNSFFLETDDSQNSIVELYQSISLRLGISVQELENIQKLNFSSVFGNPII
                     SHQ"
     gene            complement(16682..17815)
                     /locus_tag="PK252_07885"
     CDS             complement(16682..17815)
                     /locus_tag="PK252_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14668.1"
                     /translation="MLADHDRHLFEEEQLHFINHISKISSFEILKNIFDSFSVPSAIL
                     TPSRRVVYANYSLWHKLGFNSLEQIMGMRPGEAISCVYAIENHVCGVFRQCNYCGALN
                     AIKKVQSEKVEASSEMHATIFKNGEEIYFDFRVVVSPVFMDDKLYMILKLIDISNEKR
                     RKALERIFFHDVLNKVSQIKGMFEIVNKKWASNKPDEYLDMISGLIGDLSEEIVSQRM
                     LLSAENGELKVNFEGIIIQGFIESAIRQCSFFNNVQNAVFDFVPSGKAFMVFSDRTLL
                     NRIITNLLKNAMEAAYPNEVIKISVYQEDSKAKIEVCNKRLIPQDNIRRIFHRSFSTK
                     GENRGLGTYSVKLLAEKYLKGKVSVVSTEETGTIFTLELPIAK"
     gene            18076..18510
                     /gene="trxA"
                     /locus_tag="PK252_07890"
     CDS             18076..18510
                     /gene="trxA"
                     /locus_tag="PK252_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT14669.1"
                     /translation="MKKLFTTILLTTITAYNICTAIEPKSKVIHLDAETFKKKVFNYE
                     INKQWKYEGKKPAIVDFHASWCAPCRQLSPIIEEIAREYYGKIIVYKVDTDKERQLSD
                     SLGIQSLPTIIFIPVKGQPQATMGLQPKDTLKKIIKNVLLKK"
     gene            18601..19884
                     /gene="gldE"
                     /locus_tag="PK252_07895"
     CDS             18601..19884
                     /gene="gldE"
                     /locus_tag="PK252_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOT14670.1"
                     /translation="MQVYFHPLTFDTILGIIAIGILLLCSGLISASEVAFFSLRPIQL
                     DKMRQSFDTRSKKALQCLDNPEKLLANILIANNMVNIGVVILSTYVSKSLIDFSQAPL
                     LGFIFEVVVITFMILFVGEILPKIFAIDNAVAVSRMMAIPFYLLSFLFYPITILLLKS
                     TALVQKRLNNIKPELSVDDLSAAIEITSESLKEDKSILESIVKMGNISVIDIMHSRMD
                     MVALSIDTPYRRLFSSFIETGYSRIPIYENSVDNIKGVMILKDLLPYLDEDDSFNWHA
                     LIKPIHFVPESKKIDDLLAEFQKNKMHLAVVVDEHGGTCGLVTMEDVLEEVIGEIQDE
                     SDTAEEQPYKKIAANTWLFEAKIPLNDFFKVTGVNPNVFHDIKGDFETIAGLLLEMKG
                     TIPAKGTIIEHKNITFIIEQSDKRKIQLVKIIVKL"
     gene            complement(19919..21208)
                     /locus_tag="PK252_07900"
     CDS             complement(19919..21208)
                     /locus_tag="PK252_07900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT14671.1"
                     /translation="MKRACLEVTTNTYEPRFVGTSFPSQGPWGGIRLLKNNFVRMKKS
                     IPFILLACLFIPNIQSQTKLDIKLDSIFKTTGFVGLGISVYTHDSVLYQNAFGYADKE
                     SKIKYTVNTIQPIASVSKTLIAVALMKAREIGKLQLDEDINKYLPFRIENPYCPDKVI
                     TIRHLATHTSGIKDTREYEKGYIFTEKIQPIYHQMPFGLRKLLVKKYVKLYLGNQEMG
                     MEEFLSSIYVKGGKWYKKSNFSKNNPGEKVQYSNCGATLAAMIVEKATGMDYRDFVKE
                     YILTPLDMKSSCWSMLNQTHNIKSSLYLMQYKIPYYTIVTYPDGAFVTSISDFTKYMM
                     AMLKGYRGNQSILGPASCREMFAYQLKKEFKSGIFWEIKENSIGHNGSDPGVLTFAYF
                     YKEKDLGFVIFTNTSEAKDYIQKINKMVKALKESFYK"
     gene            21363..21947
                     /gene="gldD"
                     /locus_tag="PK252_07905"
     CDS             21363..21947
                     /gene="gldD"
                     /locus_tag="PK252_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOT14672.1"
                     /translation="MNKVLKLLFLMAISATAILYACSNGADTPKPKGYLRTDFETKSY
                     TILKGQYPYTFEYPTYGIVEKDTLKNAEPYWITINFKSYNSQLHLSYKKINKNLSLYI
                     EDARKMAYKHTIKADAIDEILVKRDSVKVYGIIYDIKGNTATSLQFFLTDSNRHFLRG
                     SLYFKNEPNKDSLAPSIEYFRKDVEHLINTLKWE"
     gene            21949..22575
                     /locus_tag="PK252_07910"
     CDS             21949..22575
                     /locus_tag="PK252_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT14673.1"
                     /translation="MGLYLKKYVEEDCLLGMWLIEEDFDTLYNKIQLTTEENDMLMSY
                     QSMQRRIEWLSVRVLLNEMLKNNSRIIYNESRKPFLSDNSYNISISHSLNFTSILLSK
                     TKKVGIDLEFMSHQIVNVAHKFINDKEYIDELDKRRHLYIHWCAKEALYKICDKQDIN
                     FKQNLTLEPFNIEECGVTKGIVENKFGREVFDVNYFSIDGYIIAWCTK"
     gene            22582..23310
                     /locus_tag="PK252_07915"
     CDS             22582..23310
                     /locus_tag="PK252_07915"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOT14674.1"
                     /translation="MNSSDKLYRQLLTGGPKPAVAVLVDPEKANAQSISKIVEQCKKC
                     KIDAIFIGGSLVSENPEQLISTIHQLTDIPVVLFPGSIIQISNNADAILLLSLISGRN
                     PEYLIGNHVLAAKSLYNSNLQIIPTGYILIDGGNVTSVQYISNTTPIPANKPELVVAT
                     AIAGKLLGLKAIYLEAGSGANKPVSPEIISEVKKNIDIPIIVGGGIKTHAQFTEAANA
                     GANLIVIGNSLESNPSKLSAIIRG"
     gene            23392..24900
                     /locus_tag="PK252_07920"
     CDS             23392..24900
                     /locus_tag="PK252_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT14675.1"
                     /translation="MQRKHFWILLSVLSAILVCLILIQTLWIRKSLIINNQLFKLQVN
                     GALSTVVNSLEQEETVFHITTQIPSASEETSKKTNQLLYNHNQNSHIKNNTDVSTAIS
                     AIPSENGQTAPHVSTSKACIDEYSKSIQKKAEMIERIVNRLINNQLKINERLSVKTLE
                     NIINAELMLKGIKLPYEYAIKDEKGVIALGSNNYSDTVNAERFIVRLYPNDIFNQPYF
                     LVIYFPGQQTYLYRSSWILIASSLALTISIILIISLALHIIIKQKRLSDVKTDFINNM
                     THELKTPIATISLASQMLADTNIPSSAKNIESLGKLVFDESKRLSMHVEKVLQMAMFE
                     KADLRLRMKDIHINDIITQVTNNFSIQVKSRNGKLLANLNAKEDLVHADEMHIVNIIV
                     NLLDNALKYCPADPTIIVSTRNNESGVFIDIEDNGIGMTKENLKKIFDQFYRVPTGNV
                     HNVKGFGLGLSYVKKVIDAHCGTIHVESKINKGSTFTIYIPYNQKNNKKEKL"
     gene            24897..25592
                     /locus_tag="PK252_07925"
     CDS             24897..25592
                     /locus_tag="PK252_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14676.1"
                     /translation="MNTPKIKILLAEDDENLGNLLREYLKARNYDAELVTNGEKALKS
                     LERNHYDICVLDIMMPIMDGFTVARELRKYNKELPIIFLTAKSLKEDVLEGFSIGADD
                     YLTKPFNIEELIFRIEAILKRTKNMDKDQKVYQIGSYTFDSEKQTLTLDDKVQKLTTK
                     ESDLLKLLCMNQNAVLERNFALKSIWMDDNYYNARSMDVYVTKLRKYLKDDPGIQILN
                     IHGKGFKLIIPNS"
     gene            25884..27122
                     /locus_tag="PK252_07930"
     CDS             25884..27122
                     /locus_tag="PK252_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14677.1"
                     /translation="MNTILRFLLAFSLFQFYNGLNTAKAVDEVAKLKNKLTTLSESTE
                     NSFLKLSCQSILNVIGAKQVLSENDSTLLSTMYKCFADEAADGSPSKLESYLARQRSF
                     IIAWTSPTDRAISFTWMKLPKDWNPANTYPLYIELHGLWSVANNSIDYLSYPYRNGSS
                     GSFAFEDGYLLSPWGRGNLWYKGISETDITECIGNFEKLANIDQTRKYLCGHSMGGFG
                     AWYIGQKDPSIWAALGIHAGALWYDASMTSDETISRLTDIPVYFVVGTNDGLLSINQT
                     AYNKLDAAGNENIKFVTFAGGHDYLEVNVQNMYLWMHTFTNDRVGIKSISALEEKIIL
                     LSQIKDNKAVIQCNIPESSSVSLSVTDMLGRNVAAAYIKNMAKGFQEISCDGLFIKGQ
                     IYICTLNTGKHREIVKIVVK"
     gene            27274..29169
                     /locus_tag="PK252_07935"
     CDS             27274..29169
                     /locus_tag="PK252_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOT14678.1"
                     /translation="MRKLRFILFLFLIISTKLYSQTLNDSVYSYYTDSSAYDAKYYSI
                     ELNAGTNNLYISGHTTILAQNTNKPLTKFFIQLNSSLTVDSVTIDGQKCAFTHYQHWI
                     KANPTLSISPNKLFRVTVYYKGYSATYSDFGGLSISSYNSKKILYTLSEPYSSLDFFA
                     CKQLLTDKADSVQITLNVPKGFVGIANGTLIKKSDKPDNYTSYTWKTSYPIAYYLIAI
                     AISDYKEYSYRFANTKGDSILFRNYYYNTPDFLKENKTEIDRTIDFIKYFEKITGIPY
                     PFAKDGYGHVTAPISGGMENQTLTMLSDFNFDLVAHELAHSWFGNYVTCSNWQDIWLN
                     EGFATYLQYLAHTEFNADNADIWISNCAKQARSDSHGTVFINDAEKWKPLNIFNYGIT
                     YMKGCMLVHMLRHEIANDTVFFKILHTYLSTYAYKTASTRDFIKIAEAVSGISLQTFF
                     DQWYYGAGYPIVDVSKKISANKFWIEAKQYSSNSTTQFKQLKLTVMLIFKDQTDSIIS
                     IDISSPSQNYTFTFSKEIESVSLNPQSRLLATINTYEYYVQDTLPDSSFFISPNPFRD
                     ELKLHLPSSEEIKNIWYYNINGQMLGNWQTKENILTLDTNKIQPGLYFFQATVDQKKY
                     TIKGIKR"
     gene            complement(29175..29555)
                     /locus_tag="PK252_07940"
     CDS             complement(29175..29555)
                     /locus_tag="PK252_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT14679.1"
                     /translation="MKKIIQTNKAPQAIGPYSQAIEINGSLFVSGQIPVDPATGNVVK
                     EDIIEQTHQVLKNLSSILSAAGYSMSDVVKTTCMLSDMANFAAMNSVYAEYFTSEYPA
                     RSTFQVVALPKGVLVEIDAIAMKG"
     gene            complement(29572..29958)
                     /locus_tag="PK252_07945"
     CDS             complement(29572..29958)
                     /locus_tag="PK252_07945"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14680.1"
                     /translation="MDNIIITADKDTPYFPSVTFDAEKGECTIAGESYMEDCYKFYTP
                     LIDWLTEYCNTHTAIVFNFKLYYFNTQSSRMVLKIIEVLMHFKEKGGNPEINWFYYES
                     DPDMIDEVEDFKRESGLEINLVAING"
     gene            complement(29999..30520)
                     /locus_tag="PK252_07950"
     CDS             complement(29999..30520)
                     /locus_tag="PK252_07950"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT14681.1"
                     /translation="MLTEITNKNIKLAYKGPFYIDILSELGSYLRMMYKSKPTTCTKL
                     YRLFFELTQNVAKYSMETSNVNIYKYSGVGSFMVEENGQQVFIKTSNLIKNEDGPILT
                     KYCSDINRMTKEQLRLYRGVRRKPQPEAKDTGAYIGIIQIGIISQNEIECVLESISDK
                     HSIFTICAKVNIE"
     gene            complement(30646..31866)
                     /locus_tag="PK252_07955"
     CDS             complement(30646..31866)
                     /locus_tag="PK252_07955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT14682.1"
                     /translation="MQEKIKILVADTSRLMLSTIKGIFDRNQPDYSVFIATEGKEACQ
                     KALNIKPDLILLDSELPILDVERVVRILKRVAKTRNIPIVLMSNSQQIDSLFDAGVDD
                     FIFKPFEEKEFLYRIKCALRLGRNLNKMMEQNELMAIQAKEMQRQYRMMEEQKKDIID
                     DITYSRRIQNAIMPERSYLLTLLPEYFLFYKPKRIVSGDFYWVAQKDNKVILAAADCT
                     GHGISGAFLTIAGTAFLNEIINYSFIDAAEILNHLRNHVMRLLHQKGLEGEAADGMDI
                     SLCIFDFQQMEVQYAGANNSIMQVKKNKELLVYSPDKMPIGIHENYDKPFKNQLIKLE
                     KGDMIYMYTDGYADQFGGPLDQKFRNKRLQRLCLEASGLSMAEQYQLFERTIDEWIGY
                     RDQIDDMLVIGIRV"
     gene            complement(31879..32271)
                     /locus_tag="PK252_07960"
     CDS             complement(31879..32271)
                     /locus_tag="PK252_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14683.1"
                     /translation="MGQEEKNIKVLVVDDSAINRHVLIEYLKIKDVVCDEVEDGEQAL
                     TYLQGNKPDVILLDLIMPVMDGFEVLDILKKQGNTIPVIVITAYLKGNTFQRCKELGA
                     AGFLNKPVKMQELYNLISQVLDQGNANL"
     gene            32550..33743
                     /locus_tag="PK252_07965"
     CDS             32550..33743
                     /locus_tag="PK252_07965"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14684.1"
                     /translation="MTNKLILLATLTIWQVCTSAQSVAPQKLTGKRYGFAFNNVTVEI
                     DTADGARIVSFKADNKEILYTGSTQDMNGSTFWPSPQSFWNWPPIAEIDNKAYAVKIT
                     GNKLKMEGKTNATHKLSVTKIFEADSRDTSFTITYILINHNTTTKKYAPWEVTRVLAE
                     GLTFFGKGTTEVTGNMKTNAVVADNTVWYHQDSTNSGSNFNKFFCDGQGWLAHANKEN
                     YLFLKKFENIEAANAATGEAEIEVYTADNHAYTELENQGAYVSVNAGDSIKWKVKWYV
                     RKIESKNDIFTGSQKLISIAKRLISLPELPDTFSTTRISQVKNQGINLYPVPVREKLT
                     VKLPDNVNKATVSIYNMQGAMVLSSDITASNSSISLKHVPAGPYVCRVYSKSGLNENK
                     FFLIQ"
     gene            34221..34931
                     /locus_tag="PK252_07970"
                     /pseudo
     CDS             34221..34931
                     /locus_tag="PK252_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(35121..36842)
                     /gene="lysS"
                     /locus_tag="PK252_07975"
     CDS             complement(35121..36842)
                     /gene="lysS"
                     /locus_tag="PK252_07975"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT14685.1"
                     /translation="MSLPGLSEQERFRRQSLDDLRKLGIEPYPAAMYVTNVSTKEILD
                     NFKPELQNFQEVSLAGRIMTRRIMGSASFAEIQDEKGRIQIYLKRDDICPGENKDLYN
                     IVFKKLLDIGDIIGVKGYVFVTQMGEISIHVRELTILSKSLRPLPIVKEKDGVVYDAF
                     TDPEQRYRQRYVDLIVNPQVRDVFVKRTKIINTMREFFNSKGYLEVETPILQPIPGGA
                     SARPFITHHNALDIPLYMRIANELYLKRLLVGGFEGVYEFAKDFRNEGMDRTHNPEFT
                     VMEIYVAYKDYFWMMDFTEEMLERVALALHGTTQVQFGDKIIDFKRPFARISMTDAIK
                     KYTGIDISGKDEDQLREICRQLNIPIDDTMGKGKLIDAIFGEKCEGEMVQPTFVTDYP
                     IEMSPLCKRHRNNPELTERFELMVNGKELCNAYTELNDPIDQLERFQEQLRLSEKGDD
                     EAMFIDYDFVRALEYGMPPTSGMGIGIDRLTMIMTNQLSIQDVLFFPQMKPEKKAVKD
                     EPAAYEALGIPAEWVLIIQQMGFNTVDMLKQASAGKLFNDLCGYNKKNKLGLVNPTME
                     QVKAWLA"
     gene            complement(36965..37411)
                     /locus_tag="PK252_07980"
     CDS             complement(36965..37411)
                     /locus_tag="PK252_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14686.1"
                     /translation="MKKLFVFVGILLMCTSIFAQRKGFAIGARIEGDEPNNLGVSLRG
                     KIHRAGMLEGIVHFYDGSVGATGLYERYINLSSSGTFDFVYGGGAHVEFTDDLGAGIA
                     GIVGLCYTFKEIPVNISVDWKPVLDIVSDVHMRARLLGVSIRYTMK"
     gene            complement(37461..38915)
                     /locus_tag="PK252_07985"
     CDS             complement(37461..38915)
                     /locus_tag="PK252_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT14687.1"
                     /translation="MIKHLMRVKPFEQLMIEANHEGHGFKRALSASNLVFLGIGAIIG
                     AGIFVLTGEVAAHYSGPAVALSFIISGIACALAGLCYAELASMIPISGSAYTYTYATM
                     GEFFAWIIGWDLIIEYLFAASTVSVGWSGYVVSFLANFGLQIPPSLTTAPFLYNDATG
                     GFEMSPYGIINLPAIFIVLAVSTLLVVGIKESARFNNIIVAVKITVITLFVIAGAAYI
                     YTANWHPFIPANTGHFGSFGWSGIFRGAAVIFFAYIGFDAVSTAAQEAKNPQRDLPKG
                     ILGSLLICTILYIIVSLVLTGIVPYTKLGVPDPIAVGVDAIGLKWLSPLIKIGAIAGL
                     SSVILVLLMGQPRIFYAMSKDGLLPSAFSKVHHKFRTPYMSTIIVGFIAMVLAGLFPI
                     GVLSKLVSLGTLLAFAAVCIAILVLRKTQPNTPRLFKTPFVPAVPILGIICCLGVTLF
                     LNAYAWIAMIIWLSIGIVVYFFYGYKHSRLRNNE"
BASE COUNT        12693 a         7699 c         7212 g        11535 t
ORIGIN      
        1 taataacctg caggtatgta ttatggcccc taccgagata cttgctgctc agcatttcaa
       61 aagcatcagc cgcatgatag atgggctgga tataaacatc aggctgctta caggctctac
      121 gcgcaagaaa gaacgaacta taatccagca agggcttaca gatggttcgt tgcagatttt
      181 ggttggcaca catgccctca ttgaagataa tgtacaattt aaaaaacttg gtttggccat
      241 cattgatgag caacaccgct ttggcgttgc ccaaagggca aagctgcgca acaagagcgc
      301 tcagcctcct catgtattgg tgatgacagc tacacctata ccacgtaccc tgaccatgac
      361 agcttatggc gaccttgatg tatcagttat tgacgaattg cctcccggtc gtaagcctat
      421 ttatacaaaa cacattggta tcaatcatcg gatggatatg tacaggttgg ttgcccgcga
      481 gatacaaagc ggaaggcagg tatatatagt ttatccttta atcaaagaat ctgaaaacct
      541 tgattacaaa gcattagaaa aaggatatga acagatggtg aagatatttc ctgccccaca
      601 atattctatc agtgctattc atggcaaaat gtcaaatgaa gacaaagaaa gtattatgaa
      661 agcctttaaa gaaggtaaaa ttaatatcct tatggctaca acagtaattg aagtaggcgt
      721 agatgtaccc aatgcatcgg tgatgattat tgaaagtgcc gaaaggtttg gtttatcgca
      781 gttgcatcag cttagaggaa gagtagggcg aggagctgat aaaagctatt gttttttgtg
      841 tacatctttc aagctttcta ccgatgcccg caaacgtctg gagataatgg aaagcacaaa
      901 caacggtttt accattgcag aagctgactg gaaacttaga ggcccaggaa acattgaagg
      961 aactgaacag agcggcatgc cattcagcct gcatattgcc aaccttggca aagaccagca
     1021 aatattgcag tatgcaagag atttggcaca aaaaattatc gatgatgacc cacatttgca
     1081 gaaaagcgaa aatgtaaaac tcaacatttt agtcagcaaa accaaaaaag aacaatacaa
     1141 ttgggaatta gttggataag tcaacgagag tgcttacttt taccgcaaaa acactatgat
     1201 ttcgttattc agaaaagaaa tatcgttgtt cttcaatacc cttaccggat accttgtcat
     1261 tggtgtttac ctggtgctaa atactttatt tctgtgggtt atacccggga gttttaatat
     1321 tcccgagtca aatcaagcct ctttgcatgg tctttttact atttctccgt ggctatttct
     1381 tttccttgtg cctgcaatta ccatgcgaat gatagcagac gaaagaaagt ccggaaccat
     1441 tgatttgctc tttatcaaac ccataagcga catacaaatc atagcggcca agtatcttgc
     1501 atcattagtg ttagttgctg cagctattat ccctacattc gttactttct ttacggttta
     1561 tcaccttggt tacacgcctg gcaatatgga tgtaggcgct actattggtt catacatggg
     1621 attatttttc ttagcagcta tttatgcggc tatcggagta tttgcatcat caattacaga
     1681 aaactcaata gtttcattta ttatagctgc attattgagc cttttattgt atattggttt
     1741 tgatacacta tcacaattac ctatattttc aggaatagaa gatgctataa aaagtatagg
     1801 aattaatgaa cattacaatt ctattagccg tggtgtaatt gatacacgtg atttgatata
     1861 ttttgcaggg gtttctgcca tcttcatttt attagctact tattctatcg agaaaaagca
     1921 tcaatagcat ttttgctata tacctttttt cagggatttt atgacaaaaa acaaatcaga
     1981 tatttgtatt ttaagaagtg gatataaaaa tagtgtgtgt atatttttac ccgcctgttt
     2041 tgctattatt tcgtgaaata tctttatttt taattcttca aaaacaaaat ttagaatttc
     2101 aatttagtta tgaggaaatt attgttaatc accagtatgc tatgttatat ggcaggagca
     2161 tggagccagg atgtagcttc aaaaaaagta gttaaccgcg acaacaaccc atcaaaaaag
     2221 tatttacggc cttctattac tgtcttattt gcaaaacgca acaatgacag gtcagccaga
     2281 atggaaaaag tgtttcaggc tgcaaaaaca caagataagt acaataacca caacgtttca
     2341 acctttgcta ttgaaccatc taatgattat tcaaaatatc tttcagaaaa tgtatcaaga
     2401 caaatcgtgg ctaaatggtt caatcgtaat gaaaaaggcc agtttgacat gaaccttatt
     2461 gcagaacgag gtttttacaa tgctagtgat gctgatgtgt taaaggccaa tgcttctgta
     2521 cgaaaagatg ccttgttggc tgatgcagga gagaatcttt tagacagaag ttatattctt
     2581 atttacgaca tagaccctat actgaccatg gatgaatact acaaaagaat agggacaccc
     2641 aataatttaa tggaaggtta cgctgttcaa tacaaggtgt cggtttacaa actcgactgg
     2701 acagactctg tagcagctgt gttttacaat atgtggacag atgcttcaag tcctgaccca
     2761 aacaaagttg cggcttttga taagaccaac ttcccggtaa agtatgtggg tcaggtttct
     2821 acagcttttg gctgggttac ttcatcgcaa ttcaaagacc acagtaagaa tatctttggt
     2881 tcttatagtg atgatgagct ttttgcaagg atttaccctt tgattgaaaa agaaacttcc
     2941 atctctcttc ctaagctcaa tgaagacttt agggtcaaaa cccctgttta ttcaacgcat
     3001 cctatagctg caaaaattgg gttaaaagag gggctttctg tagataagcg tttctttgtt
     3061 tacgaatacg aattgaccaa tagtggcgag aaaaaggctg tacgaaaagc ggttatccgt
     3121 gccacttcac gtattgctga caatagaaaa gttgcaaccg gccgtagcga cagctcagtg
     3181 ttttaccagg ttgccggacg taaagtttac gaagggatgc aattgcagga aaacccagat
     3241 tggggtatcg gattaaccgt aggatatggt tcaacaagcg gagctatagg tgaaggattg
     3301 acaggtatcc tcgaaatgaa tgcatcgctt tgggctggaa atgtgtttaa aggtttgcct
     3361 cccggaatta agatatatgc ccaagggtct ttggctaccg atgagtttac agtagaaaat
     3421 ggcattctcg ataagaaata taatatcact tcctatgcgt ttggtattag taaagacctg
     3481 aattttctgc accattttgt attagtgcct tttgtgggtt atggacagga acaattagtc
     3541 aatactgaaa ctgacgatga aaaatggaag aatgacaaat atgatggcac atttctgcat
     3601 tttggcgcaa ggtttgccat gaacctgaat tacaactttc agataatagc ctgttacagt
     3661 ttgaattctt atttgtcttt agaacccaat acagataatc caaacaaaga aagtcaaata
     3721 gtgaaagatt gggctgaaga acttaaaaaa ggccgaggct taggacaatt aactattggt
     3781 ttgaggtacc agttttaacc tgccagtttg aaagtattag tttatattaa atatgaattt
     3841 acattataaa ccttaattat tttattatga aaaacagaat tttaaccgtt acattaggac
     3901 ttgccttctt gttaacaggt gttgtttcaa catttggaca agccagaaag aaagccatgc
     3961 gcgacatgga agattaccgt tatgagttag agtgcgtagg tgttggagcc caaggcacat
     4021 atcttatcaa ggtatggacc tactctaaaa aacctaaagt agctattgcc caggctaaga
     4081 aaaatgctat tcacggtgtt attttcaaag gtttccccgg cggaggacaa gggtgtactt
     4141 cacaacaacc atttgcaact cctgccgatg aagacaaaaa catcgacttc tttgaaaaat
     4201 tctttgctga tggtggcgac tatatgagat atgttgccga atcaagcgat ggctctattg
     4261 gcgctgaaga ccgtcttaaa gtaggaaaag aatacaaaat tggcattgtg gtttctgtac
     4321 aaaaagacct tttgcgtaaa gaccttgaaa aagctggtat tatacgtgct ttagactcag
     4381 gtttttaatt cacattttta gtcgtaagcc ttatgtataa ctgaaagctt cagaactaat
     4441 tgcactttta aaattgtttc tcaagctgat aaaattaaaa aaaagaaaca tccatgacct
     4501 ctggcaactg agtttgcggg ctcatacgat ggtacaaaat atgtggtgtt ttcccgtcaa
     4561 agacggggca aataatcatt ctattaaaat aaaaaaataa atatgatgaa aaaaattatt
     4621 atttctcttg cagccatcat gatgtgtgtg gcatcttttg gacaggcaaa aaaacctacc
     4681 cttatggttg ttcccagtga taactggtgt aaacaaaacg gttttacaat gaaatttgat
     4741 aaccagggta ctattgaaga gcttcccgat tacaaaaaag cattacaagg gagtagtgac
     4801 cttatgcttg ttactgcaaa aatcagcgaa ttgatggcca accgtggttt cccatgtaaa
     4861 ttgctggagc aggaaatgaa aaaacttgct tctgaaagtg ctgaagatgc aatgttgacc
     4921 agcaaacaag gcggtggcgt ttcagaaagc cctatcgaca aactggccaa aacagctaaa
     4981 gctgacatta ttattcaggt tacttgggca gttaatacac aaggtcccaa aaaatctgta
     5041 actttcatcc ttcagggtat tgatgcatat accggcaaac aggttgcttc agcttctggt
     5101 acaggcgctc cttcgttttc agccgaattg cctaaattat tggaagaagc agttttaaat
     5161 tacatagaca actacaacgt tcaattacaa aaccactttg acgatatgtt caaaaatggc
     5221 cgcgaaatca ttatccgcat taagaaattt gacacctggg ccgatgattt tgaaacagaa
     5281 gttgatggga aagaaattag cgcccacatt gaagaatgga tgcagaaaaa cactgtttcc
     5341 ggccgtttca atacttcaga tgcaacagcc aacatgatgc tttttgaaca agtgcgtatc
     5401 cctatgtacg atgctaacgg caaagctctt gatgctcgta gcttctgcaa aggtttacag
     5461 acattcttca aaaaccctcc tttcaatgta accagcaagc tgatgaccaa aggtcttggc
     5521 caggctacta tcgttgttgg agataaataa gaatttacaa aaggttgctc gtgaagtttt
     5581 ttcagggcag ccttttttat tcaatcatct aaatacaaga aaatgaaaaa gttatcaatt
     5641 atacttttat cggtttttgc tttcacggtt atggccaaag cccagaacaa tgaagctaaa
     5701 gctgatgatt atgcccgtat agctttgcat tcggcagttc caaatattgc tgatatgcct
     5761 gaggcagcta aaaaactgtt gaaaaacaag ctcgatcagg tagccaccaa taccggagca
     5821 ggtggtaacg ctaagaaccc gcgttttatt attacagcaa acgtttcagt gcttaccaaa
     5881 gacattacgg caacagcacc tcctatgaca gctatcaccc ttgatattac tatctttatt
     5941 ggtgatgcac aaacgatgac caagtattca agcgcttcta tccaggctaa aggtgttggc
     6001 accaacgaaa ccaaggctta cattgaagct atcaaacaaa ttcgcgccaa ctcccctgag
     6061 ttgaaaaatg ctgttgaaac aggtaagaga aaaatcattg aatactataa ctcgcaatgc
     6121 gactttatta tcaaaaaagc tgaaactctt gccagtcaaa gcaaatttga agaagctatt
     6181 tttgaattaa gctctgtacc tgatgtttgc aaagattgct acaacaaaag catgaatgct
     6241 gttggcccta tctataaaaa atatatagac aaggcttgca aagagaaact taatgctgct
     6301 aaaggtgttt gggctggcgc tcaaaactgg gatggagctc aacaagtatc acaaatcctt
     6361 ggtactgttg accctgaagc tgcttgtatc aatgaagttc aggatttcat tgctgaagta
     6421 aaacaaaaaa tcaaagaaat ggaccaacgc gattgggact tcaagatgaa ggttttcgat
     6481 acctctgttg agctcgaaaa acaacgtatt gaggctgccc gtcaggtagg tattgctttt
     6541 ggcgaaaacc aaccagacac cgaggtgaat atcgttggat ggaaataatt cattctttta
     6601 taaaacaaaa aagcggaatc tatatggttc cgcttttttt tgtgcttttt tcaacctctc
     6661 caagggtttt aaacccttgg aggagttgat gttatttaac tcaaatactc attggtacta
     6721 accactttgg catagaagta attcaaagcc gccaaaaaac tgttttgcac gttttcggca
     6781 gtgatagatt tgttctgaat tgttaaatct ttggtagcgc aagcatcagc aatgagggta
     6841 acttcaaagc caaagtcctt agctgctctt actgttgcat caacacacat ttgcgtcatc
     6901 atgccacaaa cggtgaggtt ggtaacgttg cgttcctgca atagtttcag caattcggta
     6961 tccctgaaac tgttcggata gtgcttaatt actacctttt cgtctttcaa cgggaataca
     7021 tgtttatgaa tttcagaccc aacggtatct ggcaagaaaa atgttgaacc aggccttttg
     7081 gatatatgcc tgatatggat tacaggccaa tggttttctc taaaatgttc taacactttt
     7141 gcggcattca ggcttgcttc atacgaccct ttcagcggat tagcaccatt ctcgaaatag
     7201 tcgttctgaa tatctataat tattaatgcg tttgacatat tttcagtttc tatcaaagtt
     7261 acaaaaattg tgtttatttc tttaaattac taatgactaa ttacagatga cagttgacta
     7321 atgaaaaggc agtaagattg gctattgggt tgtaatgttg atttgcaaac gtttttatca
     7381 aacgccgaca ggtctaaaag acgctgtcgg gttaattcta tatctgacct gacagcgttc
     7441 gcagaagctg tctgcgtcta aacaattgat atctgttcat tgtatttatc attcgcaatt
     7501 ctatatcgtt tagttaagaa cacacccttc gactccgctc agggtgactg tcaccctgag
     7561 cggagtcgaa gggtgcatag cgattgaaga attgaaaata aaaccttaac taaacgacat
     7621 tgattcgtaa tttgtcatca gtcattagta atcagtaatt agtcattatc tttgaagctc
     7681 aaaatagcat ttgttgcatg ttctggaaaa atagaaacgg tgaatcatta tcggggtcct
     7741 ttttgattaa aaagaaatac cgtaaaacgg ttttatattc actattagcc agcgctttaa
     7801 tcatcattat tatttcggtg gggttgctta tttattttaa aaacagtaat aaacccaaac
     7861 gtcttttgcc tccaaaagtt gtagctgcag aacttaccag gcttaacatt gccatacctg
     7921 agatgaaaga agagcctaaa caggtgctta aaaaggcaaa agaaaagatt aaagggcaac
     7981 ctgttgaaaa aagtgaagag cctgttgttc ctgtagagac tgctaaagtt gaaaaatcag
     8041 attcagcaaa acttgccgaa aaagaatcta accttgaagc tcaagatccg cggctaaaag
     8101 aagagggcgt ttttcaatgt aataacaccc aggatttcag gctttggttt atcaatcact
     8161 tcacatttcc ccttgacgaa ggggtaaaaa agagtgaagg gcgtataaca cttgatttta
     8221 tggttggttt aaatgggctg gttgatacag tgattattat ttcaggcatt gaccctattg
     8281 tggacagtga agtaaaagct gtgatataca attcgccacg ttggaaaact tgtccgattg
     8341 atggcaagcc cgttaaacaa aaatatcgct tcactgttta tttagtccgt cggatgtaag
     8401 tttgtgaggg gacagaaaaa tgaaagtgtc aaactttttc agagtagaag gcatgagctg
     8461 tgcggcttgt gcttcgagcg tggaatctat tgtaggagct gtagaaggtg taagcttggc
     8521 atcggtcaat tttgcagcta atacgttgat ggtagaatac gatgacgaga aaacgtcttt
     8581 agacattatt gttagtgctg ctggcaatat tggatatacg ctcattgaag atattggtat
     8641 ttctatagaa caggaagaga agaagcagga gcaaaagctc aggtctatgc gatacaggct
     8701 tatcggatca tttgttttcg ctgtgccggt gtttatattg tccatgtttc tgcatcattg
     8761 gcattacagt gtttatctgc aatccatatt gtctttgcct gtagtgttat ggtttggcaa
     8821 aggcttttat atacaagggt ttaaaaaaat atttacccgc tatgccaata tggacacgct
     8881 tgtggctgtt ggtaccgggt cggcctttat tttcagtata ttgaaccttg ttttttctgg
     8941 ttatataata aataaaggtt ttgagccgca tgtttatttt gaatcggctg tgattattat
     9001 atcttttgtt ctacttggcc gatatattga agaattatca agaaacaaag ccagtgagtc
     9061 gataaaagga ttgttgttat tgcattcaaa aactgctata gtaataaaag acggacagga
     9121 tgtagaggtc tcggcagata caattcaaac aggagatatg gtgagggttt tgcccggggc
     9181 tcgtgtcccg gtggatggta ttattgtttc tggccaaacc agcatagacg aaagcatgtt
     9241 aacaggcgaa agcatgcctg tggataaaac tgccggggac aaagtgattg gggcaactgt
     9301 aaaccttact ggcagcatta ctgtagaggc tttgcgtgta ggttctgcaa ccatgctggg
     9361 caatatcatc aggcaggtgc gtgaagccca gggcagtaaa gccaaagtgc aacacatggc
     9421 cgacaaaata gcttctgtgt ttgtacccac agtatttgct atatcgctgt tgacctttgc
     9481 tatatggttt ttcgcagcgc aggacaataa cgtattcagg gcttttcagg cattctttac
     9541 ggtacttatc attgcatgtc catgtgcatt gggattggct acaccaacgg ctattgtagt
     9601 ggcagttggc aaagcttcat cacatggggt gtttgttaaa gatgctgctt tgtttgataa
     9661 agctggtaag attgatgcta ttgctattga taaaacgggt acattaactg aaggtaaacc
     9721 tgtagtaatt gattttattg attattcagg caaagaaaac gggttatctg caatatttgc
     9781 tgttgaaaaa cattcggaac atccagtatc aaaggctatt tgccattttt tgaataatag
     9841 gaataatgaa tcacttgttg tagaagattt tgaagctgtt cccgggaaag gaatcaaggc
     9901 aaaggtgaac aatgcatggc actttattgg aaatatgtca ttcatggccg aaaatggcgt
     9961 agttatttcc gaacctcata aaatagctat agatgccctt gatgataaag ccagaactgt
    10021 tgtgattgcg gctaatatga ataatttggt tgccattttt ggtattgccg atacggtgaa
    10081 aagcaatgcc gctgaagcga taaggctctt acaacacgaa ggggttgaag tccacctgct
    10141 atcaggtgat gataaaaagg tggttgccgc tgttgccgga cagcttggta ttgaccaata
    10201 caaaagccgc atgttgcctg ctgataaagc atcatatatt gccaatttac agcaaaacgg
    10261 caattgtgtg gctatggctg gcgatggcat caacgatgcc ccggcacttt cgaaggctga
    10321 cattagtttt gccatgtcta acggtacaga cattgccgca tattcggcta tggcaacatt
    10381 aatgcatggt gacataacca agatatcgtc tattatcagt tattcgcgca aaacgatgcg
    10441 aataatcaaa gaaaacctct tctgggctta cttttataat gtattggcta ttcctgtcgc
    10501 agctggcatc ttatatccaa tatgggacat acagctaaac cctatgattg ccggagctgc
    10561 aatggcattt agttcggtaa cggttgtgct gaatagtttg aggctaaggt ggtacaggtt
    10621 ttaggatatt ttattttaaa tttcattttt aaagtaattg tccaccattc aaaaattggc
    10681 ataattttcc cctatccatt ataaagtctt tcaaaaacct tacgccatgc cattccggct
    10741 tttgttgggg caaaggtttc attatgccag ataatacaaa gcgttccttg gtatttctca
    10801 acagtctgtt tcagattgtc aataatttcc tgtgcttgct caggggtata tttcagatag
    10861 gtagaaaaag tagcatccat agcaacaaac ggcaccaaaa gcaaaggtgt ttccttttcc
    10921 ttttgaaggt cataaaacat aaaagggtga caagtaccag ccctaaagcc ataatcagag
    10981 gcatagccca tcgagaagtc ttcttttatg ccatatttta tcaagtgctg ataagtatca
    11041 ggcaaatgta atttaatata atgctgcctg tttgctgaaa ccttgcggtt tgaaacattc
    11101 tcaagcatag tcaattcttt tcctatcagg taatcccgtt tctttttgct ggacgcatac
    11161 gaggcatgaa gcccaatatg agcgtattct gcaacattag aaatgacttt agccatcatt
    11221 ccttgtttta acgaaatgtt ggtgtcgtac tttgaccttt tcccagaaag aaaaaagaaa
    11281 taaggctttg gatgacagtt atacaggcaa gtacgtaaaa acacaaaagt atcataaggg
    11341 tcagccttca ttcgcatcaa ggtacgcaag cgatcctgaa aggtataaat gcgcaaagtc
    11401 aggatatccc ggacaaatgc aaagaaagtc ctgaacaccc cgcggtattt ataagcaaaa
    11461 gcattatcaa tgtcaatagt aggctggttt tgaaaatgat gcacagtaaa agtgatatcc
    11521 tgatacctgt ttttcaaaat ccttttcaac tcttctgtcc agatatccac caaaggcttt
    11581 cggtacaatt catgcttaaa aaggacacta cactctggcc taaacctatt gtgatgatct
    11641 ttttcaaatg gcagatattc ttcatacctt gaaataacga aaaatatcga agaaaaaata
    11701 tcgaaaccgg catcacaacc atcttctgtt tcagccttaa acagaaccgg catttcgttg
    11761 ataaaatcta ctcctgcgta aacaggttga atgccctttt tgtaaaccag acctgccggg
    11821 tgaatgtaca actcatcagc atatttcgta cgcgaatagt tcaatttaaa accctggaat
    11881 tttaaaaagc tctcttcatc gttggtaatg ataggcctaa gccctaacat ctttgaaaat
    11941 acatgcctta agacatacat caacctgttg ttcaattcat tgacaagaat ttgaatttca
    12001 cctgtaggtt ccatctggtt ctttttactg atttctaaag gtaggaaata tttaatattt
    12061 gagccttttc aaaatgacca atatcatctt tggacttgaa aaaatgctgt aatattgcaa
    12121 aaaactgata acatgcgtat agacatcctt actgtggtac cgggcctgtt gcacgggcct
    12181 ttggatcatt ctattgtaca aaaagcccgt aaaaaagggt tggtagatat tcatatacac
    12241 aacatacgcg attattctaa tgacaagcac cgaaaagttg acgattatgc ttttagtggt
    12301 ggtgctggca tggtaatgac cattgaacct attgataatg ctataagtgc attgaaacaa
    12361 gaacgtgaat acgacgaaat catttatact tcacctgatg gcgaaaagtt taaccagaaa
    12421 atggcaaata tgctttcgac caagaataat attctcatac tttgcgggca ttacaaaggc
    12481 attgaccagc gtgtgcgcga ccatctcatt acccgcgaag tttctattgg cgattatgtg
    12541 cttaccggcg gcgaactggc cgcagcagtc atgacagatg ccattgtcag gctgttgccc
    12601 ggcgcaatag gtgacgaaca atctgccctg tctgattcat ttcaggataa tctccttgca
    12661 cctcctgttt acacaagacc tgcggaatat aaaggttgga aagtgccgga tatactacta
    12721 tcgggcaata ccaaattgat tgatgaatgg aacctccagc aatcgataga acgcacaaag
    12781 aagctcaggc cagacctgtt ggaataattt gaaaatttgc gaattagcta atttgaaaat
    12841 gtaaaaagca attttaacca ttagcaaatt ttcaaattag ctaattcgca aattaatgta
    12901 gtaactttga ataataatat ctttactatg acaacattag aacttaaaac caaatttcat
    12961 aaattgattg acaacatcaa caatgatgat gtcttatcca aattttatga aatgctttca
    13021 aaagctaatg aagttaaaaa aggtagttta tggaacagat taaccgatga agagcagcaa
    13081 gagcttattt tattagaaaa agaaagccat aatcctgata atctaacggc ccatgcagtt
    13141 attaaaaaaa acataaaaaa tggctttaaa acaatttggg aattagttaa ttagctaatt
    13201 tgaaaatggt aagtattttt caattcattt actaattatc acattttcaa attcactaat
    13261 tgtcttaaag ccccgttatc cgcttgatgt agtttagttt atttagcgcc tccagtggtg
    13321 taagggtatt tatgtcgagg tttttaattt ggtcgcgtat ttgtttgagt acaggatctt
    13381 ctaactgaaa aaagctcaat tgataacctt cccgcttggc tgtagtctca ggagccggac
    13441 gctgaggttt atcgctacgt tgcgaatctt caagttcttt cagtatctca cctgcacgct
    13501 taacaacact cttaggcatg cctgccattt gggctacgtg gatgccaaag ctgtgttcac
    13561 ttccgccacg caccagttta cgaaggaaaa tgatattgtt gttcagctct ttgatagata
    13621 cattatagtt tttgatacgt ttgaaagagt tttccatctc gttcatttcg tggtagtgcg
    13681 tagcaaaaag agttttactt ttagccgtag gatgttcatg gatgtactcg gcaatagccc
    13741 atgcaataga tataccatcg taagtgctgg tacctcggcc aatttcgtca agcaaaacaa
    13801 gacttctggc cgagaggtta ttcaaaatac tggccgtttc aatcatctca accataaaag
    13861 tagattcgcc agaagaaatg ttgtctgaag cccctaccct ggtaaacact ttgtcgataa
    13921 tgccaatatt agcagcttta gccggaacgt agcagcccat ctgggcaagg attacaatta
    13981 atgctgtttg tcttaacaat gccgattttc cggacatgtt tggccctgta ataatcataa
    14041 tctgctgttc aacagtatca agcaatacat cattagccac atagctgttt cccggaggca
    14101 attgtttttc aatgacagga tggcggcctt ctttaatttc gagtacatcg ctatcattga
    14161 ccacaggctt ggaatagcca ttctctgctg acacaaaggc aaaagccaaa aggcaatcga
    14221 gctgccctat aaggttagca ttaagctgaa aagcctgaac atattcactt aagaaatgaa
    14281 taagttcttc aaataattga ctttcaagaa cagtgatacg ttcttcagca ccaagtatct
    14341 tttcttcgta tgttttcagc tcctgtgtaa tatatctttc agcactgaca agagtttgct
    14401 ttcgaatcca gtcggcaggc actttatctt tatgggtatt acgaacctca ataaaatagc
    14461 caaaaacatt attaaaactt attttgagag aagggatacc cgttcgttca gcctctcgtt
    14521 gctgcaattg cagcaaataa tctttgcctt tagtttgtat ggacttcaat tcatccagtt
    14581 cttcattaat tccagggtta aagattgtgc cttttccaat ctgtacagga ggttcttcaa
    14641 caaggctttt tgaaatacgc tctataacag aacgacaagg gttaagctgt tctgaaattc
    14701 ttaccatctc aggcatgccg ctttcagcac atatttgttg caaaggaatt atagcctcca
    14761 aggcattttt aagctgaatt acttctctcg gattaatgcg gctaacggct attttagaag
    14821 caagacgctc caaatcgcct ataactttga tatgttcagc ggcaatatca cgtatatcgc
    14881 tttgcgaaat gaaatactca actatgttta atctgcggtt aatagaatca acatctttga
    14941 gtggaagcat caaccaacgc ctcaacatac gtgcccccat tggagataac gtcttatcta
    15001 tcacatccag caacgatttt ccatcttcat tcatactacg caccaactca aggttgcgaa
    15061 tggtaaactt gtcaagccac atgtactttt cttcttcaat tcgcgaaata gatgttatct
    15121 ggcttatttg attttgctcg tttagctcaa ggtattgcag tattgccccg gcagaaacaa
    15181 ctcctgccgt aatttcatgt atgccaaagc cctttagcga tgtagtattg aaatgtttca
    15241 gcaacttgtc atggcagttg tcatattgaa aaacccagtc gtcaagtttg tacgtataaa
    15301 acttattccc aaagttgtca acaaaatcct ttgttttact tttttcaaaa agcacttcct
    15361 taggctgaaa attggacaac aacttgtcaa catattcatt agtcccttgg gcaacaagga
    15421 attcaccggt tgatatatca agaaaagcaa tacctgtcaa tgttttttca aagtgcaaac
    15481 aagcaataaa attattttcc ttacgttcca gaacattgtc attgtaggta atgcccggag
    15541 taatcatttc ggtaatacca cgcttgacta tttttttggt tagcttagga tcttcaagct
    15601 gttcgcagac tgcaacacgc tgacccgctc ttaccagttt atgcaggtat gtttcgatgg
    15661 catgatgagg gaaacctgct aattcaacaa aagatgcggc cccgtttgcc cttctggtaa
    15721 gagtaatacc aagtatttca gaagttttta tagcatcttc gccaaaagtt tcataaaaat
    15781 cacctaccct gaacaacaaa atagcatcag gatgtttact cttgaaacta tagtattgcc
    15841 tcattaatgg cgtatctgcg taattcccgg attctgtcac tgttgtattt ttcaacaaaa
    15901 ataggacgat aagcggtttt tcacccaagg caaaaaaaat agttgtcaac gagaaataaa
    15961 caaaggagga ttacgaatga ctaatgacta atttcaatta caaatgacaa attactgatg
    16021 actaatgata ggatttccaa atacagaact aaaattcagt ttctgaatat tttctaattc
    16081 ctgaactgaa atgccaagcc ttaacgaaat tgattgatat agctcaacta tagagttctg
    16141 actgtcatct gtctcaagaa agaagctatt cttgtcaagc atttcaataa actcagcatt
    16201 gaaaaacttt ttagagatag aaaccattaa accattatct attacttgct tgtacaaaac
    16261 agctttaccc tgaaaaccat gaataatcca tttttgctga ggttttattt gctttttaag
    16321 ttgaaatata acatcaaaag cctttacaca atgaatgacc aacggtttcc ccaacatttc
    16381 ggatagttcg atgtgtttct taaaaacttc caattgtaag ctccattctg cgcccctgat
    16441 tttatcgagc ccggtttctc caatacaaag gatattgaga ttaatagcaa gctctttgac
    16501 ttgtttcatc tgctctgaga tgctgaaatc gtcacgtata taccatggat gaatgcctac
    16561 agaataaaat ttttgtggat ttattgaatt tattttatcc cgaaagaatg taatattttc
    16621 aatagccaaa acattggcat cttcattaca gtaatgtgta tgaaaatcaa tatacataag
    16681 cttacttagc tattggcaat tctaacgtaa atattgtacc agtctcttcg gttgatacaa
    16741 ctgacacttt acctttgaga tatttttcgg ccaataactt aacactgtat gttcccaatc
    16801 ctctgttttc gccttttgtt gaaaacgaac ggtgaaaaat acgcctgata ttgtcctgcg
    16861 gaataagcct tttgttacaa acttcgattt ttgctttact atcttcctga taaaccgaaa
    16921 tttttatcac ttcattagga taagcagctt ccattgcatt ttttaacaag ttggtaataa
    16981 tgcggttaag cagggttcta tcggaaaaca ccataaaagc tttgccagac gggacaaaat
    17041 caaatacagc attctgaaca ttattaaaaa atgaacattg acgaattgcg ctttcgataa
    17101 agccttgtat tattatacct tcaaaattca ctttcagctc accattttcg gcagacaaca
    17161 acatacgctg cgaaactatc tcttcagata aatcgcctat taagcccgaa atcatgtcca
    17221 gatattcatc cggcttattt gaagcccatt ttttattgac aatctcaaac attcccttta
    17281 tttgagaaac cttattcagt acatcgtgaa aaaaaatccg ctcaagggcc ttacgcctct
    17341 tttcattgct aatgtcaata agctttaata tcatataaag cttatcatcc ataaaaacag
    17401 gagatactac taccctgaaa tcaaaataaa tttcttcgcc attcttaaat atagtggcat
    17461 gcatttcgct tgaagcttct actttctcgg actgaacttt tttgatggca ttcaacgccc
    17521 cacagtagtt gcattgacga aaaactccac aaacatggtt ttctatcgca taaacacaag
    17581 aaatagcctc acccggacgc attcccataa tttgttcgag agaattgaag cccagcttat
    17641 gccataacga ataattggcg taaacaactc ttcgtgaagg agttaaaatg gcagaaggca
    17701 cagaaaaaga atcgaaaata ttcttcagga tttcaaatga actgattttt gagatatggt
    17761 taataaagtg taattgctct tcctcaaaca aatgtctgtc atgatctgct aacataattt
    17821 cttttttttc aacattttgc ttttcaaact agaaaatgta aagcacaaaa aattggttaa
    17881 acattttttc tacaataaag atattccctt ttcgggaaaa acatttgtaa attataattt
    17941 ttatctcctg cttccattgt caaaaaagct ttgagttttt acaactaaaa aggtactttt
    18001 gcgtaaaaaa tagagttatt ctccagtacg ttaaaacgaa agactttagc agtctgacat
    18061 atgcggcaca acaatatgaa aaaacttttt acaaccatac ttttaaccac tataacagca
    18121 tataacattt gtactgcaat agagcccaaa agtaaagtga ttcatcttga tgcagaaact
    18181 tttaaaaaaa aggttttcaa ttacgaaatt aacaaacaat ggaaatacga aggtaaaaag
    18241 ccggctattg ttgactttca tgcatcatgg tgtgcgccat gccgtcaact ttcgcccatc
    18301 attgaagaga tagcaagaga atactatggt aaaataatag tttacaaagt tgacaccgac
    18361 aaagaacgtc aattatcaga ttctttaggc atacaaagtt taccaaccat tatttttatt
    18421 ccagttaaag ggcagcctca agccactatg ggcttgcaac ctaaagacac actaaaaaaa
    18481 atcatcaaaa atgttttatt aaaaaaataa acaacttctc ttacaattgt ttattgccct
    18541 tttaattttg cattcagtgt tttgttgaaa atacttacat ttgttaagta ataaagtgtt
    18601 atgcaagttt attttcatcc attgactttt gatactattc ttgggattat agcaattggc
    18661 atacttttgt tatgttcagg gcttatatca gcatctgaag tagctttttt ctcgttaagg
    18721 ccaatacaac tcgacaaaat gagacaaagc tttgacaccc gttcaaaaaa ggccttgcaa
    18781 tgccttgata accctgaaaa acttttggcc aatattctta tagctaacaa tatggtcaat
    18841 attggggttg taatactatc tacatacgtt tccaaatcgc tgattgactt ctctcaggcc
    18901 ccattattag gcttcatttt tgaggttgtg gtaataactt tcatgatact gtttgtaggg
    18961 gaaattcttc caaaaatatt tgctatagat aacgctgtgg cagtatcgcg catgatggct
    19021 atacccttct atctgttgtc attcctgttt tatccaatta ccatactact acttaaatct
    19081 acagcattgg ttcagaagag acttaataat ataaagcccg agctatctgt ggatgatttg
    19141 agcgctgcta tcgaaattac ttcagaatcg ctcaaagaag ataaatcaat actcgagagc
    19201 attgtaaaaa tgggtaatat cagtgttatt gacatcatgc attcgaggat ggatatggta
    19261 gccctatcta tagatacgcc ctatcgaagg ctgttttctt cttttatcga aacaggatac
    19321 tcgcgaatcc ctatttacga gaattctgtt gacaatatca aaggggtgat gatattaaag
    19381 gatttgcttc cttaccttga cgaagacgac tcatttaact ggcatgcttt gatcaagcct
    19441 atacattttg ttccggaatc aaaaaaaata gacgatttac ttgctgaatt tcagaaaaac
    19501 aagatgcacc tggcagttgt tgttgatgaa catggcggga catgcggcct tgtaactatg
    19561 gaagatgtgc tggaagaggt tattggcgag atacaggacg aatcagatac tgccgaagaa
    19621 caaccataca agaaaatagc tgcaaataca tggcttttcg aggccaaaat accgctcaac
    19681 gacttcttca aagttacggg agttaatccc aacgtttttc acgatattaa aggggatttt
    19741 gaaactattg ccgggctgct gcttgagatg aaaggcacaa tacccgctaa aggtaccatc
    19801 attgaacaca aaaatatcac cttcatcatc gaacagtccg acaaaagaaa gattcaactg
    19861 gtcaagataa ttgtaaagct ttaatagact ttagtggtta gtgtataggg gctagttatt
    19921 acttgtaaaa gctttctttt agcgctttca ccatcttgtt gattttctgg atatagtctt
    19981 ttgcttcgct ggtattggta aatatcacaa atcccaggtc tttttcttta tagaaatacg
    20041 caaaagtcag cacaccaggg tcgctgccat tatgaccaat tgaattttcc tttatttccc
    20101 agaaaattcc acttttaaac tcttttttaa gctgataagc aaacatctcg cggcagcttg
    20161 cagggcctaa gatgctctga tttcccctat acccctttaa catagccatc atgtactttg
    20221 taaagtcact aatactggtc acaaaagccc catcaggata agtaacaatt gtgtaataag
    20281 gtattttgta ttgcatcaaa taaagggatg atttgatgtt atgagtttgg ttcaacatag
    20341 accaacaaga actcttcata tcaaggggag tcaggatata ctcttttaca aaatccctgt
    20401 aatccatgcc tgtagctttt tcgacaatca tagcagccag tgttgcccca caattgctgt
    20461 attgcacctt ctcgcccgga ttattcttag aaaaattgct ctttttgtac cacttgccac
    20521 ctttaacata aatactggaa agaaactcct ccatgcccat ttcctggtta ccaaggtaca
    20581 gctttacata ttttttcacc aacaactttc tcaacccaaa cggcatttga tggtaaatag
    20641 gctggatctt ctctgtaaat atatatccct tctcatattc tcgtgtatcc ttaattccgg
    20701 aggtatgtgt tgccagatgc cttattgtta ttaccttatc aggacaataa ggattttcaa
    20761 tcctaaatgg caggtatttg ttaatatcct catcaagttg tagcttgcct atttcccggg
    20821 ctttcattaa agccacagca ataagggtct ttgaaaccga ggctattggc tggattgtat
    20881 taacggtata tttgattttg ctctctttgt cggcatagcc aaaagcattt tgatacagaa
    20941 cgctgtcatg agtataaaca gatatgccca acccaacgaa tccggtagtt ttaaatattg
    21001 aatctaattt aatatcaagt tttgtctggc tttgaatatt cggaataaat aagcaagcaa
    21061 gcaaaataaa gggaatagat tttttcatcc gaacaaaatt attttttaaa agccggatgc
    21121 ccccccaagg gccctgcgaa ggaaaactcg tcccgacaaa ccggggttca tacgtgtttg
    21181 tggttacttc taaacatgct cgtttcatgg actgtttttg atttcgaaaa gacaaaacaa
    21241 aaacaaatat ccctcatata aagcgaaaaa caaataaaac ttttgtcata caaatttgtt
    21301 ttgtaaaatc atggttcaca gatgagcaaa aattgtaatt ttatcgcctc aaaaacaaat
    21361 acatgaacaa agttttaaag ttattgtttt taatggcaat ttcagcaaca gctattttat
    21421 atgcctgtag caatggagct gacaccccaa aacctaaagg gtatctgcga actgactttg
    21481 aaacaaaatc atatactatt ttaaaaggcc aataccctta cacttttgaa taccccactt
    21541 atgggattgt tgaaaaagat acgttaaaaa atgctgagcc ttattggatt accatcaatt
    21601 tcaaaagtta caacagtcaa ctccatctaa gttataagaa aataaacaaa aacttatcac
    21661 tatacattga agatgccaga aaaatggctt ataaacatac tataaaagca gatgccatag
    21721 acgaaatatt ggttaaaaga gattcagtca aagtatatgg gattatttat gacattaaag
    21781 gcaatacagc tacgtcgctc cagtttttcc tgaccgatag caatcgtcac tttttaagag
    21841 gctcattata ctttaaaaat gaacctaata aagactcact tgccccttct attgaatatt
    21901 tcaggaaaga tgttgaacac cttatcaata ctttaaagtg ggaataacat gggattgtat
    21961 ttaaaaaaat acgtggagga agattgttta ttgggcatgt ggctcattga agaagacttt
    22021 gatacgcttt acaacaaaat acaacttaca acagaagaaa atgatatgct tatgtcatat
    22081 caaagtatgc aaaggcgcat agaatggctt agtgtaaggg tcttacttaa cgaaatgcta
    22141 aaaaataatt cgcgtattat atataacgaa agccgaaaac cttttctttc agacaactca
    22201 tataatatca gcatttcaca ttctttaaat ttcacgtcta tcttattaag caaaaccaag
    22261 aaagtaggaa ttgacctgga atttatgtcg caccagattg taaatgtagc tcataaattc
    22321 atcaatgata aagaatatat tgacgaactt gacaaaagaa ggcacctgta cattcattgg
    22381 tgtgcaaaag aagcgctgta caaaatatgc gacaagcaag atattaattt caaacaaaac
    22441 ctgacccttg agccttttaa catcgaagaa tgcggtgtaa ccaaaggtat tgttgaaaac
    22501 aaatttggac gcgaagtatt tgatgtaaat tacttcagca tagatgggta cattattgcc
    22561 tggtgtacaa aataaatact aatgaacagc tcagacaaac tatatcggca attacttaca
    22621 ggaggcccaa aacctgctgt tgcagtattg gtagatcctg aaaaagcaaa tgcccagagc
    22681 atttcaaaaa ttgttgagca atgcaaaaag tgcaaaatag atgctatatt tattggaggc
    22741 agccttgttt ctgaaaatcc cgaacaacta atttctacaa tacatcaatt gacagacatc
    22801 ccggtagttc tttttccggg aagcatcata caaattagca ataacgcaga tgctattttg
    22861 ttgttatccc ttatttcggg aagaaacccc gaatacctta tcggcaacca tgtacttgca
    22921 gccaaaagcc tgtacaacag taacctgcaa attattccta ccggctatat actaatagac
    22981 ggcggaaacg taacttctgt ccaatacata agtaatacca cccctatacc tgccaacaaa
    23041 cctgagcttg ttgtggctac tgccattgca ggcaagttgc ttggtttgaa agcaatatac
    23101 ctcgaagccg ggagtggcgc caacaagccc gtttctccgg aaattatttc ggaagtaaag
    23161 aaaaatatag acatcccgat tattgttggc ggaggcatca agacacatgc tcaattcacc
    23221 gaagctgcta atgccggggc taatcttatt gtgattggga attcattaga atcaaaccct
    23281 tcaaaactat cggccattat cagaggctaa acaaagctat ttgtttgggt ttaacacact
    23341 ttaacgtccc ccaatcggat ggttcaatac attcaattaa ttttgttcat tatgcaacga
    23401 aaacatttct ggatattact ttctgttctt tcagccattt tggtatgtct gatacttatc
    23461 caaaccttat ggataagaaa atcgcttatt atcaacaatc agttattcaa actgcaagta
    23521 aatggagccc tgtcaacggt tgtaaattct ttagagcagg aagaaacggt ttttcatatc
    23581 acaactcaaa taccttcggc ttcagaagaa acttctaaaa aaacaaacca acttttatac
    23641 aaccataacc aaaacagcca tatcaaaaac aataccgatg tttcaactgc catttcggcc
    23701 ataccttcag aaaacggtca aactgcccca cacgtatcca cctctaaggc ctgtattgac
    23761 gaatactcaa aatctattca aaaaaaagcc gaaatgattg aacgcatcgt aaaccgcctt
    23821 atcaacaatc aactgaagat taacgaacgg ctatcagtca aaacccttga gaacatcatc
    23881 aatgcagaac ttatgctaaa aggcattaaa ctcccatacg agtatgcaat taaggatgaa
    23941 aaaggagtta tagcattagg ttcaaacaat tacagcgaca ctgttaatgc cgaacgcttc
    24001 attgttaggc tgtaccccaa cgacattttc aaccagccgt attttctggt tatttatttt
    24061 cccggacaac aaacatacct ttatcgctcg tcatggatat tgatagcctc atctttggcc
    24121 cttactattt ctatcatttt aattatctct cttgccctgc atatcattat caaacaaaaa
    24181 cgcttgtctg atgtcaagac agactttatc aacaacatga cccatgaact aaaaacccca
    24241 atagcaacaa tatctctggc ctcgcagatg ctggcagaca ctaatatccc ttcttcggca
    24301 aaaaatattg aaagccttgg caaattggta tttgacgaga gcaaacgtct aagcatgcat
    24361 gttgaaaaag tattgcagat ggccatgttt gaaaaggccg atttgcgcct gagaatgaaa
    24421 gatattcaca tcaacgacat tattacacaa gtaacaaata acttctctat tcaggtcaaa
    24481 agccgtaacg gtaagttgct ggccaatctc aatgccaaag aagatttagt tcatgccgac
    24541 gaaatgcata ttgtcaatat tattgtaaat ttattggaca atgccctaaa atactgtccg
    24601 gcagacccaa ccattattgt ttctacaaga aataacgaat cgggcgtttt tattgacatt
    24661 gaagataacg ggataggaat gacaaaggaa aacctcaaaa aaatcttcga ccagttttac
    24721 cgtgtaccta ccggcaatgt acacaatgta aaaggctttg gcttagggtt aagttacgta
    24781 aaaaaagtaa ttgatgccca ttgtggcaca atccatgtag aaagtaagat aaataaagga
    24841 agtacattta ctatatatat tccgtacaat caaaaaaata acaaaaaaga aaagttatga
    24901 atacgccaaa aataaagatt ttattagccg aagatgacga aaatcttggc aacttgcttc
    24961 gcgaatatct aaaagcccgc aattatgatg cagaattagt cactaatggt gaaaaagcct
    25021 tgaaaagcct tgaacgcaac cattatgaca tctgcgtact cgatatcatg atgcctatca
    25081 tggatgggtt tactgtagcc cgcgaattac gtaaatataa caaagagctg cctatcattt
    25141 tccttaccgc caaatcgcta aaagaagacg tattggaagg cttctcaata ggtgcagacg
    25201 actaccttac caaacctttt aatattgaag aacttatatt tcgtattgag gctatcctga
    25261 aaaggacaaa aaacatggac aaagaccaaa aggtttacca aattggcagt tatacatttg
    25321 actccgaaaa acaaaccctc accctggacg acaaagtgca aaagcttacc accaaagaat
    25381 ctgatttgct caagttgctt tgcatgaacc aaaatgctgt gcttgaacgt aattttgccc
    25441 taaaatctat ctggatggac gacaattact acaacgcccg cagtatggac gtgtatgtca
    25501 ccaagctcag aaaatacctg aaagacgacc cgggcattca aatcctcaat attcacggca
    25561 aaggctttaa gctgattatt cctaatagtt aggtgatagg ggttaggagt taggagttag
    25621 gggttagtaa atcaatgtcg tttaattaag tattaaaaaa acgccgacag gtctgaaaaa
    25681 cgctgtcggg ttagtttcac atctgacctg acagccttcg caaaacctgc cagagcctta
    25741 actaaacggc attggatagt aaatataaat tacaacaagc taccgcaggg gtggtttaca
    25801 aagcctgggt aaactttatt catattttca ataagggtat agttctttaa gattgttata
    25861 taacaaacta aaagaatttt gccatgaata ctatcctcag gttcttatta gcatttagtt
    25921 tatttcagtt ttacaatggt ttaaacacag caaaagccgt ggatgaagta gcaaaattga
    25981 aaaacaaact cactaccctt tctgaaagta ctgaaaacag ctttctgaag cttagttgtc
    26041 aatctattct caacgtcatt ggcgcaaagc aagttctttc tgaaaacgac agtacgctat
    26101 tgtcaacgat gtataaatgc tttgccgacg aagcagccga tggaagccct tctaaacttg
    26161 aatcatacct ggcacgccaa cgctctttca tcatagcatg gacctcccca acagacaggg
    26221 ctatttcatt cacctggatg aaacttccaa aagattggaa ccccgccaac acctacccat
    26281 tgtatattga attgcacgga ttgtggagtg ttgcaaataa ttctatcgac tacctctcct
    26341 atccatacag aaatgggtct tccggaagct ttgcttttga agatggatac ctgctttccc
    26401 cttggggacg cggaaacctt tggtacaagg gtatttcaga aacagatata actgaatgca
    26461 tagggaactt tgaaaaactg gccaatattg accaaacccg gaaatacctg tgtggacatt
    26521 ccatgggtgg ctttggtgca tggtatatag ggcagaaaga ccctagcatt tgggcagcac
    26581 ttggcatcca cgctggcgct ctttggtacg atgccagcat gaccagcgac gaaacaataa
    26641 gccgattgac agacattccc gtgtattttg tagtaggaac aaacgatggc ttgctaagta
    26701 ttaaccaaac agcttataat aagctggatg cagctggaaa tgaaaatata aaatttgtca
    26761 cttttgccgg aggacatgat taccttgagg ttaatgtaca aaacatgtat ttatggatgc
    26821 atacctttac caatgacagg gttgggataa aaagcataag tgctttggag gaaaagataa
    26881 tattgctctc gcaaataaaa gacaataaag ctgttataca atgtaatatc cctgaatcca
    26941 gttccgtgag ccttagtgtt acggatatgt tgggaagaaa tgtagcagct gcttacataa
    27001 aaaatatggc taaagggttt caggaaatca gttgtgacgg tctttttata aaaggacaaa
    27061 tatatatatg taccttaaat actggcaaac atagagaaat tgtaaaaata gtggtaaaat
    27121 gaggcaaaat gatatgatta attagttctt aaattgtgtt ttaaggtttt ttattggtta
    27181 gttttatttc aaaaggcagg gttaaacaat cctgcctttt ttgagttttt tacacaaaag
    27241 ttccttatct tagtagataa aaggtcagga tagatgagaa aactacgctt tatattattc
    27301 ctttttctta tcatttcaac aaaactttat tcacaaaccc tgaatgacag cgtttatagc
    27361 tattacacag atagttctgc ttacgatgcc aaatactatt ctattgaatt gaatgccgga
    27421 accaacaacc tatacattag cgggcacaca acaatactgg cccaaaacac aaacaaaccg
    27481 ctgacaaaat tctttattca gctcaacagc tcacttacag ttgattcggt cactattgat
    27541 ggccagaagt gtgcatttac ccattatcag cattggataa aagctaatcc aacgctgtcc
    27601 atatcgccca ataagctttt cagggttaca gtttactaca aaggctattc tgcaacatat
    27661 agcgattttg gaggcctcag catcagcagt tataattcaa aaaaaatatt atacacgctt
    27721 tcagaaccat attcatcgct ggattttttt gcctgcaaac aacttttgac cgataaagct
    27781 gatagtgtac aaataacatt gaatgtacct aaaggctttg ttggtattgc caatggtaca
    27841 ctaataaaaa aatccgataa acctgacaac tataccagct acacctggaa aacctcttac
    27901 cctatagctt actacttaat agcaatagca attagcgatt ataaggagta ttcctatcgg
    27961 tttgccaaca ccaaaggcga tagcatattg tttcgcaact attattacaa cacaccggat
    28021 tttttaaaag aaaacaaaac tgaaattgac aggaccattg attttataaa gtattttgaa
    28081 aaaataaccg gcatccccta tccttttgca aaagatgggt atgggcacgt tactgccccc
    28141 atttcaggag gaatggaaaa ccagacattg actatgctca gcgactttaa ttttgacctg
    28201 gtagcccacg aacttgccca cagttggttt ggcaactatg ttacttgtag caactggcaa
    28261 gatatatggc tcaacgaagg gtttgccact taccttcaat atcttgccca tactgagttt
    28321 aatgcagaca acgcagatat ctggatatcc aattgtgcca agcaagccag gtctgacagt
    28381 catggcactg tttttatcaa tgatgctgaa aaatggaaac cgctcaatat tttcaactat
    28441 ggcattacct acatgaaagg ctgcatgctt gtgcacatgc ttaggcacga aatagccaac
    28501 gatacggtat tcttcaaaat cctgcacacc tatttatcta cctatgccta taaaacagct
    28561 tcaacccggg attttatcaa aatagccgaa gcggtttccg gtattagcct gcaaaccttc
    28621 tttgaccaat ggtattacgg ggcaggttat cccattgtcg atgttagtaa aaaaatatct
    28681 gcaaacaaat tttggataga ggccaaacaa tactcctcaa acagtaccac tcaatttaaa
    28741 caactaaaac tgacggttat gctgatattt aaagaccaaa ccgatagcat tatatctata
    28801 gacatttcaa gcccttcaca aaactatact tttacttttt caaaagaaat agaatctgta
    28861 agccttaacc cgcaaagccg tctgctagct acaataaaca cgtatgaata ttatgttcag
    28921 gatacattgc cggatagctc attttttatt tcgcccaacc ctttcagaga tgaactaaaa
    28981 ttacacctcc cttcttcaga agaaataaaa aatatctggt actacaatat aaatgggcaa
    29041 atgttgggca attggcaaac caaagaaaat atcctgactt tagataccaa taaaatacaa
    29101 cccggcttat atttttttca agctaccgtt gatcaaaaaa aatacactat aaaaggaatt
    29161 aaacgataga gctgttaacc tttcatggct atagcatcta tctctaccaa gactccctta
    29221 ggtagtgcaa ccacctgaaa tgtggaacgt gcaggatact cgctggtaaa atactcggca
    29281 taaacgctat tcatagctgc aaaattggcc atatcagaaa gcatgcaggt tgttttcaca
    29341 acatctgaca tactataacc ggctgctgac agtattgacg aaaggttttt caacacctgg
    29401 tgcgtctgct caataatatc ttctttaaca acattgccag ttgccggatc taccggtatc
    29461 tggcctgaaa caaacaaact tccgtttatt tcaatagcct gactatatgg gccaatagct
    29521 tgcggggctt tgttggtttg aattatcttt ttcattttaa aaaaatctgc gttagccgtt
    29581 tatggcaaca aggttaattt cgaggcctga ttcgcgtttg aagtcttcta cttcgtcaat
    29641 catatccggg tcactttcgt aataaaacca gttaatctca ggattgcctc ctttttcttt
    29701 aaaatgcatc aatacttcaa taatctttag caccattcgt gaagactggg tgttgaaata
    29761 gtacaattta aagttgaaca cgatagcggt gtgagtatta caatactctg tcaaccaatc
    29821 aataagtggt gtgtaaaact tgtagcagtc ttccatgtac gactcccctg ctatcgtaca
    29881 ttctcccttt tctgcatcaa aagtaactga tgggaaataa ggggtgtctt tgtctgccgt
    29941 aataattatg ttatccatta ttaattttat tacatctaaa actgatgaag taactatttt
    30001 attcaatatt aacttttgca caaatagtaa aaatcgaatg tttatcagat attgattcaa
    30061 gtacgcattc aatttcattt tgtgaaataa taccaatttg gattatccca atgtatgctc
    30121 ctgtatcttt ggcctcaggt tggggtttac gccttacacc tctatacaac ctcaattgct
    30181 ctttggtcat gcggttgata tcgctgcagt acttagttaa aataggcccg tcctcatttt
    30241 ttatcaggtt agatgttttt ataaacactt gctgcccatt ttcctccacc atgaaactac
    30301 ctactcctga atacttataa atattgacat tgctggtttc catagaatat ttggcaacat
    30361 tttgggtcaa ttcgaaaaaa agccgataaa gtttggtaca ggtggttggt ttagatttat
    30421 acatcattcg gagataactg cccaactcgg acaatatatc aatataaaaa gggcctttat
    30481 aagcaagttt aatattttta ttggttattt ctgttagcat gtgctataac attaatcagg
    30541 ctattgaaaa tcatttccaa tactgacaaa tatagtatca tttttaaatt ttaatctatt
    30601 gctaaatact ttttacaaaa atctttctca ttttgaccga tttatctaca ccctgatacc
    30661 aataaccagc atatcatcaa tctgatccct gtaacctatc cattcgtcaa tagtacgctc
    30721 aaaaagttga tattgctcag ccattgacaa acctgaagct tcaaggcaca accgctgaag
    30781 ccttttattc ctgaattttt ggtcaagagg gcctccaaac tggtcggcat atccatcggt
    30841 gtacatatat atcatatctc ctttttcaag tttaataagc tggttcttga aaggcttatc
    30901 ataattttca tgaattccaa taggcatctt gtctggcgaa tatacaagca actctttgtt
    30961 tttcttcacc tgcatgatag aattattagc cccggcatat tgtacttcca tctgctgaaa
    31021 atcaaatata cagagcgata tgtccatacc atcggccgcc tcgccttcaa ggcctttctg
    31081 gtgtaataaa cgcatcacat ggtttcgcaa gtggtttaat atttcggctg catcaataaa
    31141 tgaatagtta ataatctcat tcaaaaaggc agtaccagca atggtaagaa aagcccctga
    31201 tatgccatgc cctgtacaat ctgcggcggc aaggataacc ttattgtctt tttgtgccac
    31261 ccaataaaaa tcgccactta caatgcgttt aggcttataa aacaaaaaat attcgggcaa
    31321 aagagtcaat aaataagagc gctcgggcat aatggcattc tgtatccgcc tcgaataggt
    31381 aatatcgtca ataatgtctt tcttttgctc ttccatcatg cgatattgac gttgcatttc
    31441 tttggcctga attgccatca actcgttttg ctccatcatc ttgttcaggt tccggccaag
    31501 ccgtaaggca cattttatcc tgtaaagaaa ttctttttct tcaaatggct tgaaaataaa
    31561 atcatcgacc ccggcatcaa agagactgtc tatctgttga ctgtttgaca tcagcactat
    31621 cgggatatta cgggtttttg caaccctttt caaaatacgt acaacccgtt ccacatcaag
    31681 tattggcaac tcactgtcta aaaggattaa gtccggctta atgtttaaag ctttctgaca
    31741 ggcctcttta ccttctgtag caataaatac agaataatcc ggttgatttc tatcaaaaat
    31801 acccttgatg gttgaaagca tcaatcttga agtatcggca accagtattt tgattttctc
    31861 ttgcaaaact gtgaaatatt atagattagc attgccctga tccaacacct gcgaaattaa
    31921 attatagagc tcttgcatct ttaccggctt gtttaaaaaa ccagctgcac ctaattcttt
    31981 acaacgctga aatgtattgc cttttaaata tgcagttata acgataaccg gaatagtatt
    32041 tccctgtttt ttcagaatat ctaaaacctc aaagccatcc ataacgggca ttatcaagtc
    32101 aagtaatatc acatctggct tattgccctg aagataagta agggcttgtt ctccgtcttc
    32161 aacctcatcg catacaacgt cttttatttt caaatactct ataagcacat gccggttaat
    32221 agctgagtcg tcaactacca gtacttttat gttcttttct tcctgaccca tgagtttttt
    32281 atttcgattg gtaacttcaa aaatagcgaa aaaagactga taaaattaag ggtttttact
    32341 actttctaaa atatattttc acaaagtaac aaataaaaaa tattaacact gaaaagtgtt
    32401 ttttaatcat tagctgtatt gaacaaattg aaacattttt gaccaagccc ggatatgcag
    32461 aaaatggaca aattaacata tttttaaaaa gagccgcgta tttatactgt aaattgcaaa
    32521 ctgctaaaat caacaataat aaaacaatta tgacaaacaa actaatactg cttgctacgt
    32581 taaccatctg gcaagtttgt acttctgccc aaagcgttgc gccccaaaaa ctaacaggaa
    32641 aaagatatgg ctttgctttt aacaatgtaa ctgttgaaat agatactgct gatggggccc
    32701 gcattgtatc attcaaagcc gacaacaaag aaatattgta caccggctct acccaggata
    32761 tgaatggttc tacattctgg ccttcgccac aatcattttg gaactggccc cctattgctg
    32821 aaatagacaa taaagcatac gctgtaaaaa tcaccggcaa caagctcaaa atggaaggta
    32881 aaaccaatgc tacccacaaa ttgagcgtta caaaaatatt tgaagccgat agccgcgata
    32941 cttcttttac tatcacgtat attcttatca accataatac aacaactaaa aaatatgccc
    33001 cttgggaagt tacaagggta ttggccgaag ggcttacttt ttttgggaaa ggcaccactg
    33061 aagttaccgg aaacatgaaa accaatgctg ttgttgctga caatactgta tggtatcatc
    33121 aggacagcac taattcgggc agcaatttca acaaattttt ctgcgacgga caaggctggc
    33181 tggcccatgc caacaaagag aattatttgt ttttgaaaaa atttgaaaac attgaagctg
    33241 ccaatgcagc aacaggtgaa gctgaaatag aagtatatac agccgataac catgcatata
    33301 cagagcttga aaaccaaggc gcttatgtat ctgttaatgc cggcgacagc atcaaatgga
    33361 aagtaaaatg gtatgttcgt aaaatagaat caaaaaacga tattttcacc ggcagccaaa
    33421 aacttatcag catagccaag cgtctgatta gccttcctga acttcctgat acattctcaa
    33481 caacacgtat aagccaggta aaaaaccagg gcataaattt gtaccctgtg cctgtcagag
    33541 aaaaattaac ggtaaaactc ccggacaatg taaataaagc aactgttagc atttacaaca
    33601 tgcagggagc catggtatta tcttctgata ttactgcctc aaacagctca atatcattaa
    33661 aacatgtacc agcaggcccg tatgtttgca gggtttattc caaaagcgga ttgaatgaga
    33721 acaaattttt tttgattcaa taaatactaa ctattagata tattgcttcc cggcatgctt
    33781 aaaaaaataa gcatcccggg aagtttcgtg ttatgaccaa ttttctttgt acctttgcac
    33841 aaaaaaagat gaacaggaga agaagcaatt ttataaaacc aaaagtaaaa ttaaaaacat
    33901 ctgccagcaa agggtttgac agatcagaaa gccactcagg cgaccgaaaa acagccaaaa
    33961 atttctcaga gaaaccattt gccggaaaaa acagtaaacc agcctcccgt ttttcagata
    34021 aatcaacctt tgaaaagaaa gagcgcccat caagaagctt ttctgacaag gctttattta
    34081 gcaaaagcct aaggcctcta aatgaaggta aagaagaaac attcaaaaaa actttcaata
    34141 ccagacaaag aactaaacca gaatttaaaa ccgggaagtc tattgccgaa aagcccagat
    34201 tatatgcaga taacagcacc cgccttaaca aaatcattgc tgccagcggt atttgttcgc
    34261 gccgcgaagc agatgacatg atcaaatcag gcttaatttc tgtcaatggt aaagtggtaa
    34321 ccgaactggg gaccaaagta aaacctgaag atgatgtacg ctataatggc gaaagactta
    34381 gaaaagaaaa gcttgtctat attctattaa ataaacctaa agactacgtt acaacttcaa
    34441 aagacccttt tgccaagcgt acagttttag accttatcaa aggggcttgt aacgaaaggg
    34501 tatatcctgt aggacgttta gaccgtaaca ctacaggagt attactactt actaacgatg
    34561 gagagcttac ccgtaagctt acccacccca gccaaaaccg taaaaaaata taccaggtaa
    34621 gcttagataa aaatcttaaa caaacagact attgggccat caaaaatgga gtagagcttg
    34681 aagatggctt gtttataaaa cctgatgctg ttgaattcaa catcgaaggc gacaaaaaag
    34741 aagtcggaat tgaaatccat tcaggtcaaa accgcattgt acgcaggata tttgaaaagt
    34801 ttggctatga agttgtccgt cttgacaggg tttacttctg cggattaacc aaaaaaggct
    34861 tacaaagagg cttttggcgc tttctgactg agcaggaaat aaacatgctt aggatgggca
    34921 gatttgaata aaatactaat tcgggaattt gaaaattaac aaatgaagta ttttcaatcc
    34981 gttaaaattt taatttgttg aaaaaaacaa atttatcatt agaaggcaca atatcttgct
    35041 aattatatga ttttagttgg gttgaaacag aaaagcctga aatatcaggc tcttcattaa
    35101 tctttgtcct ttgccctacc ttatgctaac caggctttca cctgttccat tgttggattg
    35161 accagtccaa gcttattttt cttgttgtat ccgcacaagt cattaaaaag tttaccggca
    35221 gaggcttgtt taagcatatc aacagtattg aagcccattt gttggataat caacacccat
    35281 tcagccggaa tacctaatgc ttcgtaagca gcaggctcgt ctttaactgc tttcttctca
    35341 ggtttcattt gagggaagaa aagcacatcc tgaatggaaa gctggttggt catgatcatg
    35401 gtaaggcggt caataccaat acccatacca gatgtaggag gcataccata ctcaagggcc
    35461 cttacaaaat cataatcaat aaacatggct tcgtcatcac ctttttctga aaggcgtaac
    35521 tgttcctgaa aacgttcaag ctggtctatt gggtcgttaa gttcggtata tgcgttacaa
    35581 agctctttgc cgttgaccat cagctcaaag cgctcggtaa gctctggatt gttgcggtga
    35641 cgcttgcaaa gtggcgacat ttcaattggg taatcggtaa caaaggtagg ttggaccatc
    35701 tctccttcac atttttcgcc aaaaattgca tcaatcagtt ttcctttacc catggtgtcg
    35761 tcaatcggga tattcaattg acgacaaatt tcgcgaagct ggtcttcatc ttttccggaa
    35821 atatcaatgc ctgtatattt tttaatagca tcagtcatac taatacgggc aaaaggtcgc
    35881 ttaaagtcga ttatcttatc gccaaactgt acctgtgttg taccatgcaa agccaaagcc
    35941 acacgttcga gcatttcttc agtaaaatcc atcatccaga agtagtcttt ataagctaca
    36001 taaatttcca tcacggtaaa ttcaggatta tgcgtacggt ccattccctc gttacggaaa
    36061 tctttggcaa actcatacac cccttcaaaa ccgccaacca acaggcgttt taaatacagc
    36121 tcattggcaa tgcgcatata caaaggaata tctaatgcat tgtggtgggt gataaaggga
    36181 cgagcagaag ctccaccagg tataggttgt aaaataggtg tttctacctc caaataacct
    36241 tttgagttaa aaaactcgcg catggtattg ataatcttgg tacgtttaac aaacacgtca
    36301 cgcacctgag gattaacaat caaatcaaca taacgttgtc tgtatctttg ctcggggtct
    36361 gtaaatgcat cataaaccac accatctttt tcttttacaa taggcaatgg cctaagcgat
    36421 ttgcttaaaa tggtaagttc tctcacatgg atactgattt cgcccatttg ggtaacgaaa
    36481 acatagccct taacgccaat aatatcgcca atatcaagga gttttttaaa tacaatatta
    36541 tataagtctt tattttcgcc cgggcaaata tcgtcacgtt ttaaatatat ctgaatacgg
    36601 cctttttcgt cttgtatttc ggcaaaagaa gcgctaccca ttatacggcg tgtcatgata
    36661 cgtccggcaa ggcttacctc ctgaaaattt tgtagttcag gcttgaaatt gtccagtatt
    36721 tctttggtcg aaacattggt tacgtacata gcagccggat atggctctat tccaagcttt
    36781 ctcaaatcat ccaacgactg acgtctaaaa cgttcttgtt cgctaagtcc gggtaaactc
    36841 atagtaaata tttgtttttg agccgcaaag ataagtgttt ttaaaaatta tttcattgga
    36901 aaatacagat acaaaaaacc ggttaataaa gatttaaccg gtttgtatta ttgtaaagta
    36961 cagtttactt catggtatat ctgatagata cacccagtag cctggcccgc atatgaacat
    37021 cgctgacaat gtcaagtaca ggtttccaat ctactgagat attcacgggt atttctttaa
    37081 aagtatagca aaggccgaca ataccagcaa taccggcccc caaatcatca gtaaactcaa
    37141 catgggcacc acctccataa acaaagtcga aagtgccgct tgacgacaag ttaatataac
    37201 gctcgtagag cccagtagca ccaacagagc cgtcataaaa atgtacaata ccttcgagca
    37261 tgccggctct atgaattttg cccctcaacg aaaccccgag gttgtttggc tcatcacctt
    37321 caatacgtgc accaatggca aaaccttttc tttgggcaaa aatacttgta cacatcaata
    37381 atattcccac aaacacaaat agttttttca taatcattaa gttttattgg tttggtaaca
    37441 aattcattat tcattattca ttattcatta tttctcaacc tgctatgctt atatccatag
    37501 aaaaaataaa caacgatacc aatagaaagc caaataatca tggcaatcca ggcataagca
    37561 tttaaaaaca gggtaacgcc aaggcaacaa ataatcccta aaataggaac ggcggggaca
    37621 aaaggtgttt taaacaatct tggagtattg ggctgagttt tgcggagtac taaaatagct
    37681 atacacacag cagcaaaagc cagtaaagta cctaaagaaa ccaacttgct caatacccct
    37741 attggaaaga gcccggctaa aaccatggca ataaaaccca caataatagt tgacatataa
    37801 ggcgtccgaa atttatgatg cactttagaa aaagctgaag gtaaaagccc atctttagac
    37861 atagcataaa aaatacgggg ttgtcccata agcaaaacca atataacaga actaagcccg
    37921 gcaatagccc caatcttaat cagcggggag agccatttta agccaatagc atctacccca
    37981 acagcaatag gatcgggtac tccaagtttt gtataaggga caataccggt aaggacaaga
    38041 gatacaataa tatacaaaat tgtacatatc agtaatgaac ctaaaattcc tttaggcagg
    38101 tcgcgctgag gatttttagc ttcctgggca gctgttgaaa ccgcatcaaa accaatataa
    38161 gcaaaaaata taaccgctgc ccccctaaat atgccactcc agccaaacga accaaaatgt
    38221 cctgtattgg ccggaataaa aggatgccag ttggcagtat aaatatatgc agctccggca
    38281 atgacaaaca aagtaattac tgttattttt acagctacta taatattgtt gaaacgggca
    38341 gactctttaa tacccacaac cagtagagta ctaacggcca aaacaataaa aatagcagga
    38401 aggttgataa ttccatacgg tgacatttca aaaccgccgg tagcatcatt gtacagaaat
    38461 ggagctgtgg taagagacgg tggtatttgt aacccgaagt tggccaaaaa acttaccaca
    38521 tatcccgacc aaccaacact gaccgttgat gctgcaaaaa gatactcaat aatgaggtcc
    38581 cacccgataa tccaggcaaa aaactctccc atagtggcat acgtgtaagt ataagcactg
    38641 ccagaaatag gtatcattga ggctaactct gcatagcaca agcctgccaa tgcacaagca
    38701 atacctgata taataaatga caatgctaca gcaggacccg aataatgagc agcaacttca
    38761 cctgtcaaaa caaatattcc agctccaata atagcaccaa tgcctaaaaa tacaaggttt
    38821 gaagcagaca atgcccgctt aaacccatgc ccctcatgat tggcctcaat catcaactgc
    38881 tcaaaaggct taacccgcat caaatgtttt atcatacaac gtaaatgttt ttactgccgc
    38941 aaattaatca ttttttggtg taatcaaaaa cattgaatga aatagcattt tgccattcag
    39001 taaaaaggtt ttataagccc aatttggaca atagcgttca gcggtaaatt aaaaaaaaac
    39061 aggctcttaa ctgatagtgc aggtctttgg ctattgaacg gggcctgcac gtacgggtca
    39121 gggctcataa accgcccca
//
LOCUS       DAOSPF010000032        38311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1108_length_38311_cov_5.841776, whole genome shotgun sequence.
ACCESSION   DAOSPF010000032 DAOSPF010000000
VERSION     DAOSPF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_38311_cov_5.841776"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK252_07990"
     CDS             <1..655
                     /locus_tag="PK252_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14688.1"
                     /translation="IARLNEMLKSVVLYPISNTLGEVRYSLKIGDKVVTTAAKPKVTS
                     VNNCAKITEVLNNQTEWVIKYNYDGCFDDPANAIVVSYSMSPSMKNNFPFDVNANKAQ
                     IFLKGNVILTKISDQGSTVNAKCRMTVISKFNSPVTIDKITLVWNNYPPLVIDNFNKQ
                     IQGKGEHELVFDLPQAIDKAKCSASAKATINGNIQYRSVVGEKSTFNIYDQDMTTDW"
     gene            1774..4932
                     /locus_tag="PK252_07995"
     CDS             1774..4932
                     /locus_tag="PK252_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14689.1"
                     /translation="MIGKNYTTKRRVSLFRGVALLLFLVLANSIFAQEKTITGTVADK
                     SGMVLPGVNVIIKGTTKGTVTDLDGKFSLRVPDNNAELLISFVGYENQEIKVNDQTTF
                     SVVLTENVKEIDQIVVVGYGVQKKSDLTGAVASVSSEKLAKTPVTGLDQALQGMAAGV
                     NIIPVTGRPGSGVNIQVRGISSINGTKPKIILDGVAQDDDALNRINPDDVESIEVLKD
                     ASSAAIYGASGGNGVIIVTTKKGKSGDIRVNYNAYMGIDNVIKKLDLMNSQQWVELVE
                     EMSTSKKPFTTNPDTFKTYDWQDIFFREAISQNHDLSISGGSDKSTFLLSSSYSKEEG
                     IIKNTDHQRFTLRLNSEHKINKYVTFEEKISYVNNVTKGFADWEYSGYYQNPVMAAYQ
                     MIPYQPAYKEDGTWTEPYGGVTNPMVGIDMKNNTMRSNNFEGNFSVNITPLKGLTYTS
                     RFTQGLNFGDNKEYQAKYFASATNRRDQNALIQSMNRGLSWNVQNILTYTNTLFDSHN
                     FSVMAGHEAGKWWGYDISGQRVDMPSDMDELLYLSMSTNDTLAKQVVEGSGYEGRSYR
                     YFGRLNYDYKGKYLLTVNVSRDYHYSFGPANRGGTFPSFSVGWKFTEEEFVKNNISVL
                     NFGKIRYGYGQTGANAKTGYPYLTTVKTPAKYMYAVNGTTASIGAGPEQIANPAIKWE
                     SINMTNVGLDLGFWDNKLTLSAEYFIKTNDGMIMQQQVPFTVGAYDMTLPEVNIGSIE
                     NRGFELTVGHKNTIGDLKYSMDFNISFIKNKIIDLATDSLLNGGVHVVSPITLTREGG
                     AVSEYYGYKTNGLFRETDPMIAKVKGTKTTYIMANQTFFIKANGDTAYFQPIAQPGDV
                     RYVDVNGDGIMDNKDKVSLGSPLPKFTFGFMVNLEYKMFDLNAFFNGSYGNKVFNGTK
                     QYLYYAQGYGNRAAAFANRYKNEVVKDGVVVVHANKDTDIPRFNSDNYAKPSDFYVED
                     GSYIRLRSIQLGFTVPKSITQKMGIGRFKVYVGAKNLFTITNYSGVDPVVSGGTNVMA
                     QGIDIGGYPATKTYLAGINLEF"
     gene            4964..6613
                     /locus_tag="PK252_08000"
     CDS             4964..6613
                     /locus_tag="PK252_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14690.1"
                     /translation="MINKNLKYISVALLAISALSCKESYLDTKPVAQQMGSTFYLTQQ
                     AGEQAVITAYAQYNNVQLDMHLVMAPDVISDDAEAGGEYVNEVPSYENLNRLIMLPTA
                     GEFETVYGSLFKSIYFANVALQYLPGILTVDKEADPAVINRLMAECKFIRALDYSYLV
                     RYFGPVALVDHPLGADEYFQKNATLKQLYDFMEKDLKEAIAVLPERSAMTEIGRASKG
                     AAKTLMAKNLLFESSYAKYYPGDERFAGMKERWAEALDYAEQVINSGQYALVGKDGGR
                     FKCWRSPKANAYRFLFTSDGDNSSEGIYEIQNIIDGKGWAEARGNAICQFGSARRIVK
                     ADGSTDNSGYWGLDLPADGLVNEFEPGDPRLRAGIAIEGNGDSIEVQGGKRYLISFEK
                     SVTKTYMTKYESSGKEFKDVGGPWHSAPMNIRLMRYADVLLMAAEAAVMLGQNDKALN
                     YINQVRTRARLCGPDGNTVPANLTGTVTLNDIIHERRVELYLEGHRYFDLVRWNLAKK
                     YLNHFTEDGYQVIYESPKHDFMPFPQKEINTNPNLKQNPGW"
     gene            6964..9864
                     /locus_tag="PK252_08005"
     CDS             6964..9864
                     /locus_tag="PK252_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT14691.1"
                     /translation="MKRRLFITVMVLVAFLCYPKSFANTWYDSAQVRIDSLRKGNFTF
                     KIVDKDGNAVQDSVKVIHRKHEFPWGISIDLPYNATVPNTYTASSAIIAPADSEVYRT
                     ERWAAYLAYLLPAEKGKKYKITVKLSENWFDAAGKRIFDTYVDGQKKIENIDKYILGG
                     GKYKPFDTSINVVATDSVIHLTFQSSLDNAAVMGIVLADSVGNAILRINCGGEAMTTK
                     SGNSYVSDLAYIDLNAKSPSTTSDDWYKAVMLKYFNYGVCGNQFKWSGIEPNRGQLNY
                     APFENTLGWFKKVGWDMRAHTLLWGGTSSTDYHELPKWVGELPGKAMYDTCKMRVKRE
                     VTRYKGIVREYDVMNEPTHATYLQSKAGDSINWNCFKWAYEADPTARFFVNDYNIIEY
                     QDQTDNFIKLVKKMLEKGAPVTGIGAQCHIGATTDIDNFKKRFDQLSQFGLPIKVTEF
                     DMNALKVSQQQHAIEAAKMMRLCFSHPAIEGFIFWGIMDPGWADGVANLLNEDRTPKI
                     VADTVYHLIHNVWTTKITDKTNASGMFTFKGYYGDYDILVKVGDTWKKYSIPYKKAFK
                     DSVFVLNEIMGVATSPEVEKVKVNAPSTIEITFNKKMKDPSADAYSFKVFDNSTNYVK
                     SAALKNGDSTTIVLTMNTAFKERQFMPVSYYPGNVESADGGKLEAFGPVLDNNLVSAY
                     LSSSTSKNGKKVLVAFNGKLADTSLNAANFAVKVNNVNNAITAIALCPTKDTVYLTIA
                     NQVVNKSDVVTVTYTPGSLITSDSMYVAAFSAKSVTNAILEAKCVAAVTDYNGTQVLL
                     SFNQYMTLPAGVENNFKIKVNGVEVAIEKAELKSNDSRILIFTLSSTFYKGDELAVSY
                     EPGTLMSVDEVVVAKFSITATNRSKIVSVESLESANAKYYPNPVEDRLYLANTSEYTM
                     AKVTDLSGKVLLRSKINMGGITELNTSNLKSGIYFIVLSNNKKQLVSKVVKK"
     gene            10177..12771
                     /locus_tag="PK252_08010"
     CDS             10177..12771
                     /locus_tag="PK252_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT14692.1"
                     /translation="MKRKTCTLLALGICMGVWGQMMPFQNPELSSQERAQDLISRLTI
                     EEKVTLMCDQSDAIPRLDIKKFNWWSEALHGYANNDSVTVFPEPIGMAASFNDELLFK
                     IYDAVSDEGRAKYHQWLRRGNENKRFLSLSVWTPNVNIFRDPRWGRGQETYGEDPYLT
                     SRMGVQVVKGLQGPADAKYKKLLACAKHYAVHSGPEWSRHQLNLNNVDPRDLYETYLP
                     AFKALVQDADVRQVMCAYQRLDDEPCCGNTRLLQRILREEWGFKHIVVSDCGAITDFF
                     TSHKVSSTPVHAASKGVLAGTDVECVWENYPYKTLPDGVKRGLLKEEDVDKSLMCVLV
                     GRFELGDFDDDKLVPWAQIPASVLNCEKHKKLAYEMAQQSMTLLQNKKNILPLSKTSG
                     RIAVIGPNANDKVMLWGNYNGTPIETVTILDGIKTKLSDKKVFYDKGCDLVEDKVTDS
                     YFGQLSFEGKKGFKVTYWNNPEMKGDAVITQYITDPIKKTTAGQHEFASGVKLEGFSA
                     LFEAEFVPKVSEELIFKGGATGFFELVVNGDTLKRYHNWRTLPSRIPLKVEAGKKYNI
                     KISYAQLNNWQANIELDFGKEVDVDYTGLINKLKGIETVVFVGGLSGNLEGEEMPVSY
                     PGFKGGDRTNIELPLVQRNCLKALKQAGKKVVFVNCSGSAIALTPETESCDAILQAWY
                     AGELGGMAVADVLFGDYNPSGKLPITFYKSSDQLKDFEDYSMKGRTYRFMDDALFPFG
                     YGLSYTTFSIGNAKLNKTSLNKGDSLVLNIPVINAGKRNGTEIVQVYVRKVNDTDGPL
                     KTLKGFKRLNVSAGKTAQAVICLPYNSFEFYDRTTGKMTVAAGEYEVFYGNSSDKKDL
                     KTVKITVQ"
     gene            12794..13936
                     /locus_tag="PK252_08015"
     CDS             12794..13936
                     /locus_tag="PK252_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14693.1"
                     /translation="MLKHTLVALLSVFSLFGVAQQPTVIEDFKPSTLNQPGKEYPMVN
                     SQGYARFRVIAPEAQSVIVSLGLGGKGGTKLVKGADSVWTGTTEGPMDEGFHYYHLTI
                     DGGIFNDPGTLNFFGSTRWESGIEIPAHDKDFYALKDVPHGNVQQVLFPSPSTNSVRR
                     AFIYTPPGYGKDKNKRYPVLYLQHGWGEDETAWSNQGHANLIMDNLIAEGKVKPFIIV
                     MTYGMTNEIKFGTLKSFDIKPFQTVLVNELIPYVDANFLTLSDRNNRAMAGLSMGGME
                     TKMITLANLDKFAYIGLFSGGTFSMEDVNNTPGFKEKVKLAFVSFGSRELEHRWPGFG
                     GGDPKENAEALKQAGINSVFYVSPGTAHEFLSWRRSLREMAPLLFK"
     gene            14063..15223
                     /locus_tag="PK252_08020"
     CDS             14063..15223
                     /locus_tag="PK252_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14694.1"
                     /translation="MNIKPIHALLTVLLASQISFAQNTPSVVKEDFRPSSVNQPGKQY
                     PQVNSEGRVRTSITAPEANKVQLDIGGVKYDLVKDANGVWTGESAPQDEGFHYYQLNI
                     DGASVPDPGTKYYYGAGRWGSGIEIPAPDQDIYALKDVPHGLVSEVVYFSKITQAWRC
                     CIVYTPAGYETNANVRYPVLYLQHGSFEDETGWSSQGKANLILDNLIADKKAVPMIIV
                     MDNGYAFKPQEGENKKPLLVFEEVMMQEIIPMIDSRFRTIADKRHRAIAGLSMGANQT
                     LRIAMNNLAAFSAYGGFSGTANYPSADEIDPVTFLDGKFAEGKAVNQQLPVFFLSLGT
                     KEPNPFPGSVGAFRKMLDKQGIKYSFYESPGTAHEWQTWRRSLYQFAQLLFK"
     gene            15253..16416
                     /locus_tag="PK252_08025"
     CDS             15253..16416
                     /locus_tag="PK252_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14695.1"
                     /translation="MKFLAIRNYLAFTILTLLLCSMGIAKAQLKENSVAATTNIAQNL
                     CPCIMPDNSVFFQVKAPNAQKVQIDLGKKYDMVKATNGVWSVRTEPVVPGFHYYFLVI
                     DDVPVADPASQSFYGTGKMTSAIDIPEKGIDFYDIKDVPHGSISSKYYYSKVDNCWKR
                     LFVYTPPGYDQNTNMKYPVIYIQHGGGEDETGWAIQGKTDLILDNLIAGKKAKPMIIV
                     MANGNSRAGIGGYTSEAMARFKKEMTQNIVPFVDENYRTLADAKNRAICGLSMGGGQS
                     FYVGLQSLEYFSSVGIFSSGIFGGIRNPSGKEFNAETEIPGLLSKSDEFNAKLDLFYI
                     SCGEQDPRIEFTKKAVDVMKKNGLEVEFNSFPGDHEWQVWRKSLHDFASRVFK"
     gene            16448..17659
                     /locus_tag="PK252_08030"
     CDS             16448..17659
                     /locus_tag="PK252_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT14696.1"
                     /translation="MNIRKILFHTLSFLLIIFPALVRGQQANVNLDYNPQKNTEGLIP
                     FSAPLNSPEVHDDQTVTFRLKAPKAQEVKLAGVAILTALGKGKEMVPFTKGDDGVWTL
                     TVGPLKPDMYAYHFNVDGVQITDPSNTYAAFTAMPPYSQLIVHGSGPAYYDAQNVPHG
                     TVTRHIYHSEVTNGERELYVYTPPGYKPDKKYPVLYLLGGSGELPSNWVYDGRVNFIM
                     DNLLAEGKAVPMIIVIPNNQVVHRNHPKHAELTFDIFEKELRNHVVPLIDQNYSTIKE
                     PKGRALSGLSMGGRHSMYIGFRSLDLFANFGILSAGDVEAEKSLAQFLNDPKVNNKVD
                     YLFVGQGTEEANGFMGQRCIALHEALARHNIEHEYYVGGYGGHDWATWRHLLYYRFLP
                     NLWKKINNIKK"
     gene            17662..19224
                     /locus_tag="PK252_08035"
     CDS             17662..19224
                     /locus_tag="PK252_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT14697.1"
                     /translation="MKQINLLVAAFVLFGIVACKKQITGQVEVEGGIIQGIVTEDLTI
                     FKGVPFAAPPVGDLRWKAPQPVVKWEGVKQTTEFAPAPMQGGNPVSGKSEDCLYLNIW
                     TPAKSVDEKLPVLVWIYGGGFSFGSTSDPVHNGEHLARKGVVVVSIAYRVGQLGFLAH
                     PELSAENPEKVSGNYGLLDQIAGLQWIQRNIAAFGGDPEKVTIFGESAGAISVSMLCA
                     SPLAKGLFRGAISQSGGSFGPTRPTTYPGENMKTLKQAEKEAESYLQMAGVKTIAELR
                     KIEADKLPAGWGMPGAWPIVDGYVIPDDQYKLYESGKYNDVAVLIGYNSDEGLSFPTG
                     RTPEEFVSNVKMRFGPFADSLLQAYPLEKNAIARSARNLMRDAAFGWHTWSWARLQSQ
                     TGKSKVFYYYFDQHPVRPEGSPQADHGTPHGVDVPYVFMNLDTANQGVTKSDIEISQI
                     MGTYWTNFAKYGDPNGDGVAQWPAFSANNPQVMYLGPNTFIGKVPDEKALQVLDGYFA
                     WRRSPEGVAWAK"
     gene            19255..20091
                     /locus_tag="PK252_08040"
     CDS             19255..20091
                     /locus_tag="PK252_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT14698.1"
                     /translation="MKKIILIITVMLGLNAGISAQDPNFYIFLCFGQSNMEGNAKVEP
                     QDTIVDSRFQVMETVNCPNLKRDKGKWYAAVPPLCRCYTGLTPADYFGRTLLVNLPKN
                     VRIGVINVAVGGCKIELFDKDNYQTYVASAPDWLQNMVKEYDGNPYARLVEMAKLAQK
                     DGVIKGILLHQGESNTNDSLWPAKVKGVYDNLLKDLNLTANSVPLLAGEVVAADQKGA
                     CASMNAIIAQLPKVIPNSYVIPSTGCPQRGDNLHFTAEGYRMLGKRYGVKMLSLLLDK
                     VQ"
     gene            20118..21488
                     /locus_tag="PK252_08045"
     CDS             20118..21488
                     /locus_tag="PK252_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT14699.1"
                     /translation="MKKLLLSFAFVGLCGFVGAQKPIVQTYFTADPAPMVYNDTVYLY
                     TSHDEDSTVNNFFTMYDWRCYSSTDMVNWTDHGAVASLKNIAWFDKNNGAWAPQCIAR
                     NGKFYLYVPIHGGGISVLVGDSPTGPFRDVLGKRLIDSEHIWNDIDPTIFVDDDGQAY
                     LYWGNPVLRYVKLNNDMISYDKSIGKNGIVSLEMTGEAFGEAVDRDGKPRTKYVEGPW
                     FYKRNGIYYMVYAAAGIPEYIAYSTATSPVGPWTYKGFIMERAPKLAFTNHSGIIDYK
                     GNSYFFYHSESLTGGGGFRRSVCLEQFKYNADGSIPLIVPTAEGVKESLSALNPYKRV
                     EAETMAWSEGLKTAKDNSKGIYVTKINNGDYIKVSSVDFGKGATTFEASLASAAKGGQ
                     IEIRLNSTDGELLGTLDVKNTKGANNWAVQSCKVKAAKGIHDLYLVFKGGEGDLFNFD
                     WWKFSK"
     gene            21535..23574
                     /locus_tag="PK252_08050"
     CDS             21535..23574
                     /locus_tag="PK252_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOT14700.1"
                     /translation="MGRIGKLMMVAILMIALSMAGTNTFSAQTVWLDELDLSAATQGW
                     GVPMKNRSIDNKTLTIAGKTFERGFATHSVSSLLILLEGKAVSFSAKVGIDDEVKGHD
                     AAAEFELYGDGKKLWSSGLMRVGDTARVCSVNLTGIKKFELVVADGGNGNYYDHADWV
                     DARFEAKGVTTFKTYNPVASEPYILTPKPSDKPRINSAKVFGVRPGSPFQYLVAATGD
                     RPMVFSAKGLPEGLKIDQNTGLITGVLSNAGTYMVTLEAKNAKGRAEKQLRIECGNRI
                     ALTPPMGWNSWNCFAHEVSAEKVKKAAEAMVKSGLINHGWTYINIDDFWENHRDSKDP
                     SLQGKFRDDAGYIVTNSRFGDMKALADYVHSLGLKIGLYSSPGPWTCGGCAGSYGFEK
                     QDAESYAKWGFDYLKYDWCSYGNVINGLPDNDPYKVSSLSYNGGGELATAIKPFKLMG
                     EYIRQQPRDIVYSLCQYGMSDVWKWGDSVGGNCWRTTNDITDTWVSVKSIALDQDKSA
                     AYAKPGNWNDPDMLVVGHVGWGNPHPSKLKPDEQYLHISLWSLFSAPLLIGCDMEKLD
                     DFTLNLLTNDEVIEINQDPLGKQATCKQTIGELRIYVKELEDGSFAVGFCNFGLDIAE
                     LSYKDFSKIGVNGKFKVRDLWRQKDISTIDSQTGQLPVKVPVHGALLYKFTPQKR"
     gene            23707..26130
                     /locus_tag="PK252_08055"
     CDS             23707..26130
                     /locus_tag="PK252_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOT14701.1"
                     /translation="MRQTIISKHTNGLEHKCSWVKSSFFIMVFALFSLSTILNAQIEN
                     FQVGSTTRKMLVYAPSTIVPGRPLLISMHGMNQDINYQKGQAQWETIAKKENFVVVYP
                     GGEGNSWDLAGDKDIKFILAIIDEMVKRYSIDRDRVYLSGFSMGGMMTYYAATKIADK
                     IAAFAPVSGYNMGGPNTKSSRPIPLIHVHGTADDVVAYSGVAASMNAWVARNGCPTTP
                     VVTKPYPVGKSTSDEKKYWGPGIDSVEVVLISLSGKGHWHSIEASGVNTSQEIWDFCK
                     KFALGFGVPKFKAATVSDVNPKQIQVTFSKPLKALNKFEGFSVKVDGQDAAIDTVLFT
                     DSLHLAINLVANILKTNEITLSYNGGNVVSVYEKPLVTIKDTAVDNQLLGSAPKLVQL
                     FVNEKGDSLIAKFTKKMLLPADLSSIVLKAKYNGEKSVSLINCSFLNNDSTTLVFPLA
                     DTVYADYKLRLSYTGNVVASSDSGMFKNDTAYVIINKSRGLPAHIVSAIIDDSAIAIS
                     LEFNKPMSMKDAQISQLLLKVNGKVTKIKEVFNVNNTIRLNLFNSLYYRDSIVITYTP
                     GTITAADSGYLEAFTDFVVENPIAAPTYTAIPGKIEAENYAMQFGTDTETTSDAGGGL
                     NVGWIDAGDWMVYAIENNTEDSVYQIKFRLATQASGSKFEYFIDDVKIGQIDVPNTGA
                     WQKFTSVIKDIIVPKGKHYFKIVDVTGNFNINYFVIQKDFVSVSQTNKDDISIFPNPA
                     SDKIVIKSAGFQYDKIEISDMMGRTVLVKSLTYAPELVLPLNLNKGTYFLKISNSKSS
                     FTQKVNVVK"
     gene            26329..28278
                     /locus_tag="PK252_08060"
     CDS             26329..28278
                     /locus_tag="PK252_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOT14702.1"
                     /translation="MAECVKRMNSSVKRWIALSCLISLTMLAFSQPVKVVSPNQKNII
                     ELFNHQDGNENRWFLKISYSRAGSNVEAVSRVDIGLLRNDQDFSNNLKFIKASKPLII
                     DEQYSMLHGKKQKCTNSANEVVVYFENSNKEKMNLILRAYNDGVTFRYEFPGKGGSYV
                     IKDELTSYHINSAYKRWIQMFDLSNEWLFKYYKNDSVQAIWGYPALFNSADSACWFLL
                     HEADVNRSYCASRLNNKADKQAYKITFPEDWEGNGEVLPIITLPWKSPWRVVIMGSLA
                     DITASTLVDDVCPSSVIVNTDWIKPGIASWNYWSNNHGTKDYKTVCEFADLAAAMGWP
                     YTLLDWEWDQMGNGGNLEDAVKYIYSKGVKPLIWYNSGAFKWITATPVDRMLTHEKRM
                     EEFAKLKKLGIVGVKIDFFLNEKQEMMKYYLDILDDAAKFNIMVYFHGCLVPRGWART
                     YPHLMTYEGIRGAEWYNNGPEFTSAAPSHNSIIPFTRNVVGSMDYTPVTFTNSQFPHI
                     TSYGHELALSVVFESGIQHMADRPSGYYSLPDAAKTFLMNVPNTWDDIKLLDGYPGHE
                     VIMARNKGSQWYIGGINGENVEKNKILKFDFLPEGAKYKLTLIADGEHDKELKAQYMV
                     VEKTSLVDVKMLRRGGFVAQLIPIQ"
     gene            28278..30911
                     /locus_tag="PK252_08065"
     CDS             28278..30911
                     /locus_tag="PK252_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT14703.1"
                     /translation="MKVLKYFILKALLVLISLFPAMVYGQAYKSHAIQKDRLSVQLTE
                     GVLDIIPLADGAVRVQYKKELLKELPELIFTNASSAVKFKVSDAESALIFSTDKMIVK
                     LHKANGSLAFSDKSGKVFLSEKENSRWLVPDSVHGEPCYKAEQSFVSPSGEALFGLGQ
                     FQDGHYNLRGVTRRLIQVNSQIALPFIYSGKGYGLLWHQYGLTDFNPADNFISLEKQE
                     ASASANNQVAEVTTTSGMQKVSQDQTAYKGKFTVPVDGEYSIFLDLGDMGNRQFVVID
                     GIPCIDQSNLWLPPSISALVKLKAGEHHVQLVCKSSNNPELSWKRNDNLTTFRSPNAT
                     MLDYVVFYGPSADSVISAYRNLSGNVPMFPQWAYGFWQCRERYTSGSHLVATVKEFRK
                     RNLPMDVIVQDWQYWGNKGWGVPVFDDVNYANPAGFIKEIHDQHAHFNISIWSNPDRN
                     STLGKDYVAKNLYVQNSKWLDYFNPVTRETYWKSLNDNLFTHGVDSWWMDATEPENDA
                     LKGAKTFYGLGDFYRLTYPMFVSQAVYEGQRKLTSEKRVCILTRSAFLGQQRYGVINW
                     SGDIGSDWDTYRRQIVAGLNYTITGFPYWTTDIGGFFRPGNGQYTDEKYHEVLTRWFQ
                     WGALNPIFRIHGFMSETEPWKFGSQVENNMRKMLDLRYRLLPYIYSEAWQVSHNGSTL
                     MRPLVMDFNGDTVAVGQPYEFMFGKAFLVAPVTEPNLKEQAVYLPKTATWYDFWTGKR
                     LNGGQTVKTEIPLDKVPLFVKAGAIVPMGKFIQYAGQKSADTLEIRVYAGADGTFGLY
                     EDEGDNYNYEKGGYSTISFEWNEKKQTLTIGQRYGDFSGCLKNRVFNVVLVNENNGTG
                     ILSGVASKQVLYSGKKINLKL"
     gene            30927..32060
                     /locus_tag="PK252_08070"
     CDS             30927..32060
                     /locus_tag="PK252_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT14704.1"
                     /translation="MGNKLKILVALTCLAAVAISPARGADKNQKLKDAFAGKFFIGTA
                     MNAEQILEKDTAAVRLIKENFGAIVAENCMKAGPIQPKEGEFDFSLADKFVEFGEKNN
                     KFITGHCLVWHSQAPRWFFKDSLGNDVSREVLIERMKKHIYTVVGRYKGRVKGWDVVN
                     EAIDDRDGSLRKSKYYQIIGEDFIKLAFQFAHEADPQAELYYNDYSLSDPKKRKGAIE
                     IVKKLKEQGVRIDAVGEQCHVGLDYPTIDELEKTITEFAALGVKVMITEMDVVVLPMD
                     WSLGADVSARVEYKKKSDLYVNGLPDSVNTVFEKRYLDLFKLFLKHKDVITRVTLWGV
                     TDANSWKNNWPIPGRTDYPLLFDRKYQVKPVVGKIISEALKNN"
     gene            32163..33932
                     /locus_tag="PK252_08075"
     CDS             32163..33932
                     /locus_tag="PK252_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT14705.1"
                     /translation="MKKENLKMRKNRQLMLLSVFVALFSFVALSGKDKGKVAKNAPVF
                     SNFVYQGNDKVYTDNPLKADEFYNPILQGCYPDPSFTRKGDDYYLVCSSFAMFPGVPI
                     FHSKDLVNWTQIGHVLDRTSQLKVEDCGISAGVYAPQILYNPNNDMFYMITTQFSGGF
                     GNIVVKTKDPLKGWSDPIKLDFNGIDPSLFFDDNGKGYVVHNDAPDPGKELYSGHRVI
                     KIWEYDVQNDKVIPGTDVIIVDGGVDINKKPIWIEAPHIYKKNGKYYLMCAEGGTGGW
                     HSEVIFVSDNPKGPYKPAPSNPILTQRYFPKDRVNKVDWAGHADLVLGPDNKYYGVFL
                     AIRPNEADRVNIGRETFILPVDWSGEFPVFENGLVPIEPKLKMPKGVENKTGKDGFFP
                     NGNFTFTDNFTTEKLDYRWIGLRGPRENFISVTKKGLQITPFATNIKEVKPTSTLFLR
                     QQHNNFSYAVTLDYKPGSEKDLAGITALQSENFNYVFGITKKGSDYYILLERNEKQRR
                     RDAALESKIMGSAKIDLKSPVRLKVEAKGDNYEFSYSLDGTTFQTLGGTVSGDILSTN
                     VAGGFTGCLIGLYATSANDAKPE"
     gene            33991..36369
                     /locus_tag="PK252_08080"
     CDS             33991..36369
                     /locus_tag="PK252_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT14706.1"
                     /translation="MKKYIIAIIGVSLFLSNINAQDKLYPNTFPLGDVVLLDGPFKHA
                     RDLNIKTLLQYDVDRLLAPYRKEAGLPEKAKSYPNWEGLDGHVGGHYLSAMAINYAET
                     GNTECKKRMDYMIAELKACQDANGLKYPDWGVGYLGGVPNSAALWSKIKAGEVGAIWS
                     AWAPFYNIHKMYAGLRDAWLYTGNEEAKNIFLKFCDWGITLIAGLDDKQMEDMLANEH
                     GGMNEMYADAYQMTGDKKYLEAAKRYSHKAFLEPLSQGIDMLDNKHANTQIPKFIGFE
                     RIFQLSGDDKYGKAGSFFWETVTTNRSLAFGGNSRREHFPSPASCIDFVSDIDGPESC
                     NSYNMLKLTEDLFRVQPSAKYADYYERTVFNHILSSQHPEHGGYVYFTPARPRHYRVY
                     SAPNQAMWCCVGTGMENHGKYNQFIYSHSHDSLFLNLFIASELNWKEKKIKIRQETNF
                     PNEEKTKLSVVDGTASFKLMLRYPAWVNKNSLKIKVNGKVFSYNALPSSYVCIDRKWN
                     KGDVVEVTLPMHNTIEHLPNVPNYIAFMHGPILLCAKTGTEDLKGLIADDGRWGQLAV
                     GEKLPVDKAPILIEDDIQHIAEKLVPIKDKPLNFSLNVKMINPIQVQFQPFYQIHDAR
                     YMMYWLALTNNGYKSYLDSLSNIEKEKIALEDRTIDKVAPGEQQPEVDHAIQKENSGT
                     GNTNGVFFRDARDNGYFSYELATNSESNLSLVVRYWGAEWGNRKFDIYIDNEKLLTED
                     NTGRWNQSMFKDVVYTIPDAMLSGKKFVRVKFQAINGNAAGGVYGIRLVRNK"
     gene            36398..36931
                     /locus_tag="PK252_08085"
     CDS             36398..36931
                     /locus_tag="PK252_08085"
                     /inference="COORDINATES: protein motif:HMM:NF023010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HOT14707.1"
                     /translation="MKTFKILALLVFVVIFAQKINAQNVNTDKKDSLYKDFKTEFVWE
                     AKVKIAGMINVGQSKRGERRVIPITGGTFKGPKIKGEVLPGGEDWQLVRPDGDTELYA
                     RYLLKTDDGYVIQVINRALIHAGKEFYCKSVLDLEAPTNSPYEYLNHAIFIGTLNVPP
                     LKQGEEPYVIIGVYKLL"
     gene            36951..>38311
                     /locus_tag="PK252_08090"
     CDS             36951..>38311
                     /locus_tag="PK252_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT14708.1"
                     /translation="MKIFLSILAICSFALIKVNAQSWTADNGNGTYSNPLFFDEFSDP
                     DIIRVGEDFYLAGTTMHAMPGLVVLHSKDLVNWEFMSYCLDSLNLGKEFNLEDGKEAY
                     GQGIWAPCIRYHNNMFYIFTNVNGHGMQVFISKDPKGPWVHKPLNTKIYDLSVLFDDD
                     GKIYAVYNYNEVRMVELKPDMSGIVEGTEKVIIPAGNNMGEGHHIYKIKGKYYIISAN
                     YAPVGRMQCARADKPYGPYETAVISAEETMGFKRGWVTWSVGLGKPVPEPGFKFKLDE
                     PGENDFGAVPLHQGGIVDLPNGDWWGFSMMDFMSVGRTTCLSPVTWHDGWPYFGLPGN
                     LGRSPRTWVKPNVAAVTSPSAPYQRNDDFSGPKLQPVWQWNHNPVLRKWELNKKKGSL
                     RLHTLPAKDFLWAKNTLTQRVVGPESYATVVLDATFLKVGDIAGLAYLNMPYACLGVA
                     RTK"
BASE COUNT        11561 a         6822 c         8583 g        11345 t
ORIGIN      
        1 tattgccagg ttgaacgaaa tgctcaaatc tgtggtgctt tatcctattt ccaataccct
       61 tggcgaagtc agatattcgc tcaagattgg cgataaagtg gttactactg ctgccaaacc
      121 taaggttact tcggtaaaca actgcgctaa aattaccgag gtgttgaaca accaaaccga
      181 atgggtgatt aaatacaatt atgatggttg ttttgacgac ccggccaacg ctatcgttgt
      241 ttcttacagc atgagcccaa gtatgaaaaa caattttcct tttgatgtga atgccaacaa
      301 ggctcaaatt ttcctcaaag gcaatgtaat ccttaccaaa ataagtgatc agggtagtac
      361 agtaaatgca aaatgccgta tgacggtcat ttctaagttc aatagccctg ttactattga
      421 taaaataacc cttgtatgga acaattatcc accactggta attgataact ttaataaaca
      481 aatccaaggc aaaggcgaac atgaactggt ttttgacctg cctcaagcta ttgataaagc
      541 caaatgtagc gcttctgcca aagctactat aaacggcaat attcaatatc gctctgtggt
      601 tggcgaaaag tcaactttta atatttatga ccaggatatg actactgact ggtagaattt
      661 atagaggtaa aattaattca atattttaga ccttcagtat gttagtgttt gttaccctgt
      721 tttaatgccg gggttgaata aaaagaatgc aaagttttag taatcaatgt taaagctttg
      781 cattcttttt tttattgttt tatcccaaat tgagcattaa aagcgcttaa tcggttaaga
      841 aattgtaatt caatgattta acaaaatttt ttgaacaatt ttttaatcaa cttgtcaaag
      901 tttataacat gaaggttaac ccaactaaat catatagttc ggtaagaaat agcttcccat
      961 tgattttaaa ttgagtttgt ctaatatggt acttcgtaaa taaatttatt gtctataacg
     1021 agtaatggta taaattttat aagaggaaaa attgttttgt attttaaaaa tctttcattt
     1081 ttaagtaaaa aaatgaaagt attacatttt ttttaataat gaagaaatat tgaaagcgct
     1141 tttgaaaggg tttaaagggt taggtatgta tatttgaaca taattaaatt acaataatat
     1201 atttgataga atgtcaattt tttgagtaga taataggtgt taacaaatct accattaaat
     1261 aatggtttgt aatttgttta tatttagtta gttgtatgag tatgggaaag ggctggttga
     1321 aattttttca agtccctttt ctttttagga tattttgatg cgtttcagga ctttaatgaa
     1381 aattcaatat tttagtttag ctaaccttat taaaaccaac ctattttttc cttaaaaggg
     1441 aaagattact ctcttatatt gggtgggttt gtgaaatata aagcctctac caaacgataa
     1501 tgaataaaga atctttaaaa gttttaatta tttaaaatcc ctgataaata aagaaaagtg
     1561 tttttttaac tatttcttaa agtttgagtt tgagcagtgg ttgtttgtta aaaatctttc
     1621 attttttgtt taaaaattga aagagtggat aaataggggg ttaaatgaac taatattaat
     1681 aatatcagca taaagattga aaggtttcgc aataggcagt ctatactttt ggtttcgtta
     1741 acaaatttta tactaactat taattaaatg tttatgattg gaaaaaatta cacaaccaag
     1801 cgtagggtta gccttttccg gggagtagcc ttattgctat ttcttgtgct tgcaaattca
     1861 atatttgcgc aagaaaaaac cattaccggt actgtggcag ataaatcagg tatggtgctg
     1921 ccaggtgtaa atgttattat taaagggact acaaaaggga ctgtaaccga ccttgacggt
     1981 aagttttctt tgagagttcc tgataataat gctgaattat taatttcatt tgttggttat
     2041 gagaatcagg agattaaagt aaatgaccaa actacgtttt ctgttgtttt aactgagaat
     2101 gttaaagaaa ttgaccaaat tgttgtagta ggttatggtg ttcagaaaaa aagcgatttg
     2161 accggggccg tagcttctgt ttcaagtgaa aaacttgcca aaactcctgt taccgggtta
     2221 gaccaggctt tacaaggtat ggctgctggg gttaatatca ttcctgttac cggaaggcct
     2281 ggtagtggag ttaatatcca ggtgagaggg attagctcta tcaatggtac aaaacctaag
     2341 attattttgg atggtgtggc tcaggacgat gatgcattga accgtattaa cccggatgat
     2401 gttgagtcta ttgaggtctt gaaagatgca tcttcagctg caatttatgg agcttcgggt
     2461 ggtaatggtg ttattattgt gacaaccaag aaaggtaaat ctggagatat cagggtgaac
     2521 tataatgctt atatgggtat tgataatgtt atcaaaaaac ttgacctgat gaactcacaa
     2581 caatgggttg aacttgttga agaaatgtca acctcaaaaa aaccttttac taccaatccg
     2641 gataccttta aaacttatga ttggcaagat atctttttta gagaagctat ctcgcaaaat
     2701 catgaccttt caatttctgg tggtagcgac aagtcaacat ttttgttaag ctcaagctat
     2761 agtaaagaag aaggtattat taaaaacact gaccaccaac ggtttacttt gcgtttaaac
     2821 tcagaacaca aaattaacaa gtatgttact tttgaagaaa agatatctta tgtaaataat
     2881 gtaacaaagg gttttgcaga ttgggaatat tctggttatt atcaaaaccc tgtaatggct
     2941 gcatatcaga tgatccctta tcagcctgca tataaagaag atggtacctg gacagaacct
     3001 tatggaggtg tgacaaaccc tatggtaggc attgatatga aaaacaatac tatgcgcagc
     3061 aataactttg agggtaactt tagtgtaaat attacccctc ttaaaggcct gacttatact
     3121 tcaaggttta cacaaggctt gaattttggg gataataagg aatatcaggc taaatatttt
     3181 gcttctgcta caaacagaag agaccaaaat gctttgattc aaagcatgaa caggggattg
     3241 agctggaatg ttcaaaatat tttaacttat actaatacgt tatttgatag ccacaatttt
     3301 tctgtcatgg ctggacatga agccggcaaa tggtggggct atgatattag tggccagcgt
     3361 gtagatatgc caagtgatat ggacgaactt ctttacttgt caatgtcaac aaatgataca
     3421 cttgcaaaac aggttgttga aggtagtggg tacgaaggaa ggagctaccg ctattttgga
     3481 cgtttaaact atgactacaa gggaaaatat cttttgactg tcaatgtcag ccgtgactat
     3541 cattacagct ttggccctgc aaacaggggc ggtacattcc cttcattctc tgttggatgg
     3601 aaatttacag aagaagaatt tgtaaaaaat aatatcagtg tgctgaattt tggtaaaatc
     3661 aggtatggtt atggacaaac cggggctaat gccaaaactg gttatcctta cctgacaaca
     3721 gtaaagaccc ctgctaaata tatgtatgcg gttaatggta ctacagctag tattggtgca
     3781 ggacctgagc aaatagccaa ccctgctatt aaatgggaat ctatcaacat gaccaatgtt
     3841 ggtttggatc ttggtttctg ggacaacaaa ctgaccctct ctgctgaata ctttatcaaa
     3901 accaatgatg gtatgattat gcaacaacaa gtacctttta ctgttggtgc atacgatatg
     3961 actttgcctg aagttaatat tggaagtatt gaaaacagag gttttgaatt gactgttggt
     4021 cataaaaata caattggcga tttgaagtat tcaatggatt ttaatatttc tttcataaag
     4081 aataaaatta ttgaccttgc cacagactct ttgttgaatg gcggggtaca cgttgtatct
     4141 cctattacat tgacccgtga aggtggggct gtgtctgagt attatggata caagacaaat
     4201 ggtcttttcc gtgagacaga tcctatgatt gctaaagtaa aaggtacaaa aactacttat
     4261 attatggcaa atcagacatt ttttattaaa gccaatggcg atacagcata ttttcaacca
     4321 attgcacaac ctggagatgt taggtatgtg gatgtaaatg gtgatggtat tatggataat
     4381 aaagataaag tatctcttgg tagtccgctt cctaagttca ctttcgggtt tatggttaac
     4441 cttgaataca agatgtttga tttaaatgct ttcttcaacg gctcttatgg caataaagta
     4501 tttaatggta caaaacaata tttgtattat gcacaaggtt atggcaacag ggctgctgct
     4561 tttgctaaca ggtacaagaa tgaagttgta aaagatggag ttgttgtggt tcatgcaaat
     4621 aaagatactg atattccccg tttcaactca gacaactatg ctaaaccatc agacttttat
     4681 gtagaagatg gttcatatat caggttgaga agtattcagt tagggtttac tgtacctaaa
     4741 tctattaccc aaaagatggg tattggcagg ttcaaagttt atgtaggggc caagaatttg
     4801 tttactatta caaattatag tggcgtagat cctgtagttt caggtggtac caatgttatg
     4861 gcacaaggta ttgatattgg aggttatcca gcaacaaaaa cttacttagc tggtattaac
     4921 cttgaatttt aatataggta aaatttaaaa aaaattaaca tttatgataa ataaaaattt
     4981 aaaatatata tcggttgctt tgcttgctat ttcggcatta agctgcaaag aaagttacct
     5041 tgatacgaag cctgtggccc aacaaatggg tagtacattt tatttgactc agcaggcagg
     5101 ggagcaagct gtaattacag cttatgctca gtataacaat gtacaattag atatgcactt
     5161 ggttatggca cctgatgtta tttctgatga tgctgaggct ggtggagagt atgtaaatga
     5221 ggttcctagt tatgaaaatc ttaacaggct tattatgctg cctacagctg gcgaatttga
     5281 aactgtttac ggttctttgt ttaaaagtat ttactttgct aatgttgcat tgcaatattt
     5341 accagggatt ttgaccgtag ataaggaagc tgatcctgct gttatcaatc gtttgatggc
     5401 agaatgtaag tttatcaggg ctttagatta ttcatacctg gtaagatatt ttggtcctgt
     5461 tgcattagtt gatcatcctc ttggtgcaga cgaatatttt cagaaaaatg caactttgaa
     5521 gcaattgtat gattttatgg aaaaagatct taaagaagca attgctgtgt tgcctgaaag
     5581 aagtgctatg actgaaattg gaagggcatc aaaaggtgca gcaaaaacct tgatggccaa
     5641 gaaccttctt tttgagtctt cttatgcaaa atattatcct ggggacgaac gttttgcagg
     5701 gatgaaagaa cgctgggctg aagccttgga ttatgccgaa caggttatta attcaggtca
     5761 gtatgcttta gtgggcaaag atggcggcag gttcaaatgc tggagaagcc ctaaagccaa
     5821 tgcgtacagg ttcctgttta cttctgatgg agataattct tctgaaggaa tatatgaaat
     5881 acagaacatt atagatggta aaggctgggc tgaagcccga ggcaatgcga tttgtcaatt
     5941 tggttctgcc cgtagaatag taaaagcaga cggatctacc gacaactcag gatactgggg
     6001 gcttgatttg cctgccgatg gtttggtaaa cgaatttgaa cctggcgatc cccgcttacg
     6061 tgccggtatt gctattgaag gaaatggcga ttctattgaa gtccaagggg gcaagagata
     6121 tctcatcagc tttgaaaagt ctgtaactaa gacttatatg actaaatatg aaagctccgg
     6181 aaaagaattt aaagatgttg gtggcccttg gcacagtgcc cctatgaata tcagattgat
     6241 gcgttatgcc gatgtgttgt tgatggctgc tgaagctgcc gttatgcttg gccaaaacga
     6301 taaagcgctt aactatatca atcaggttag gacacgggca aggttgtgtg gccctgatgg
     6361 aaatactgtt ccggccaact tgacaggcac agttaccctg aatgatatta ttcatgagcg
     6421 cagggttgaa ctataccttg aagggcatcg ttattttgat ttggtccgtt ggaaccttgc
     6481 caaaaaatat ctgaaccact ttactgaaga tggatatcag gttatttacg agtctccaaa
     6541 gcatgacttt atgcctttcc ctcagaaaga gataaacaca aatcctaatc tgaaacaaaa
     6601 tccgggttgg taaaaagtgt tctttataaa tatactcttc ctcttcagaa tttggggaag
     6661 ggtatatttg agtttacttt tttaatgatt ttagttggtt gattgtaatt aaaggaaggg
     6721 ttttgcttgt ttggcatgcc ctttctttta actattaatt aaaaaaatag tttgcattaa
     6781 taagctactt ttatttttca actatctatt ggggctgtaa cctatctgct aagaaacttt
     6841 aaatgggata aaaagcatgc tataaaagca ggtttaactt tagaaattaa aaaaataaaa
     6901 tctcttttca gtttataacg taatacatac actaatttta tactaactaa atacaaacaa
     6961 attatgaaaa gaagattatt tattacagtt atggttttgg tggcattttt atgttatcca
     7021 aagtcctttg caaatacttg gtacgatagt gctcaagtgc gtatagacag cttacgcaaa
     7081 ggtaatttta cctttaaaat tgtcgataaa gatggcaatg ctgttcagga cagcgtaaaa
     7141 gttattcatc gaaaacatga gtttccctgg gggatatcaa ttgatttgcc ttataacgct
     7201 actgtgccta atacctatac tgcatcttct gctattattg ctcctgcaga ctctgaagta
     7261 tatcgaaccg aaaggtgggc tgcatatttg gcttatttgc ttccggccga aaaagggaag
     7321 aagtataaaa ttactgtcaa actttctgag aactggtttg atgccgcagg aaaaagaatt
     7381 tttgacacct atgtagatgg acaaaaaaag attgaaaata ttgataaata tattttggga
     7441 ggaggaaaat ataaaccttt tgacacctca atcaatgtag ttgctacaga ctctgttatt
     7501 caccttacat ttcagtcaag tttagataat gctgccgtaa tgggtattgt tttggcagac
     7561 agtgttggca atgctatcct tcgtatcaat tgtggcggag aagccatgac caccaaaagt
     7621 ggcaacagtt atgtcagcga tttggcatat atagatttga atgcaaaatc tccttctacc
     7681 acttcggatg actggtacaa ggctgttatg ttgaaatatt ttaactatgg tgtttgtggc
     7741 aaccagttta agtggagcgg cattgaacct aacaggggac agcttaatta tgcccctttt
     7801 gaaaatactt taggctggtt caaaaaggta ggctgggata tgcgtgccca cacgctgctt
     7861 tggggtggta ccagtagtac cgattatcat gaattgccta agtgggttgg cgaattaccg
     7921 ggcaaagcca tgtatgatac ttgtaaaatg cgcgttaaac gtgaagttac acgctataaa
     7981 ggtattgttc gtgagtatga tgtgatgaac gagcctaccc atgcaactta tttacaatcg
     8041 aaagccgggg actcaatcaa ttggaactgc ttcaaatggg cttacgaggc cgatcctact
     8101 gccaggtttt ttgtgaacga ttacaatatt attgagtatc aggatcagac agataatttc
     8161 attaagctgg ttaagaaaat gcttgaaaaa ggagctcctg ttactggtat tggtgctcaa
     8221 tgccatattg gggccactac agatattgat aactttaaaa aacgttttga ccagcttagc
     8281 cagtttggcc ttcctattaa ggttaccgag tttgatatga acgcgttaaa ggttagtcag
     8341 caacaacatg ctattgaagc tgctaaaatg atgcgccttt gtttttctca tcctgctatt
     8401 gaaggtttta ttttttgggg tattatggac ccgggttggg ccgatggtgt agctaacctt
     8461 ttaaacgaag acagaactcc taagattgtt gctgatacgg tctatcacct tattcacaat
     8521 gtttggacta caaaaataac cgataaaacc aatgcttccg gtatgtttac tttcaaaggc
     8581 tactacggag attatgacat ccttgtcaaa gttggagata cctggaagaa atattcaata
     8641 ccttataaaa aagcgtttaa ggatagtgtt tttgtcctga acgaaatcat gggcgtggct
     8701 acaagccctg aagttgaaaa agtaaaggtc aatgccccct caaccattga aattactttt
     8761 aataaaaaaa tgaaagaccc atctgctgat gcttatagtt ttaaggtttt tgacaactct
     8821 accaattatg ttaaaagtgc agccttgaaa aatggagatt ctactaccat tgttttgaca
     8881 atgaatactg catttaaaga aagacaattc atgccagttt cttattatcc tggcaatgtt
     8941 gagtctgctg atggcggaaa actcgaagca tttgggccgg tgcttgacaa caaccttgtt
     9001 tcggcatatc tttcttcttc aacaagcaaa aatgggaaaa aggtgttagt tgcattcaac
     9061 ggcaaattgg ctgatacctc acttaatgct gctaattttg ctgtaaaagt gaacaatgta
     9121 aacaatgcta ttacagcaat agccctttgt ccaacaaaag ataccgtata tcttactatt
     9181 gcaaatcagg ttgtaaataa atctgatgta gttacagtta catatacgcc tggttcttta
     9241 attacttcgg atagcatgta tgttgctgct ttttctgcta aatctgtgac taatgctata
     9301 ttggaagcaa aatgtgttgc tgctgttact gattataacg gaacacaggt cttactctca
     9361 ttcaaccaat atatgacact tccggccgga gttgaaaaca attttaaaat aaaagtaaac
     9421 ggtgtagaag ttgccattga aaaagctgaa ctgaaaagta atgacagccg tatcctgata
     9481 tttacacttt cttcaacttt ttataaagga gatgagcttg ctgtaagtta tgaacctgga
     9541 actttaatgt ctgtagatga agttgtagtg gctaaatttt ctattactgc tactaaccga
     9601 agcaagatag tgtctgtaga aagcttagaa agcgccaatg caaagtatta tcccaaccct
     9661 gtggaggata gattgtattt ggccaatact tcagaatata ctatggctaa ggttactgac
     9721 ctttcgggca aggtgttgct tcgttcaaaa atcaacatgg gaggtattac agaacttaat
     9781 acttcgaatt taaaaagtgg tatttatttc attgtccttt ctaacaataa aaaacaactt
     9841 gtttcaaaag tggtaaaaaa ataaacattc ataacaaacc gggtgatggc aaatgctatt
     9901 gcccggttta taaaaaagat accaaagttt ggcttacaga taaaaatatc gacagaaggc
     9961 atttttcata attgattaaa tatccatgaa ggataataaa acaacatatc agacacaaag
    10021 catgaataat tcatattcgt tgtcttttcc ccgaaataaa aacattgcaa atgaaatatc
    10081 cttgtttggg caaatgctta gcaaataggc attgtccggg cagtcaaaat atagggtcta
    10141 attagaaata tagtaacata ttaatacgaa atgcggatga aaagaaaaac atgcacatta
    10201 ttagctttag gaatttgtat gggggtgtgg ggccagatga tgccttttca gaatccggag
    10261 ttaagttcac aagaacgggc tcaggactta atatctcgtc ttacaattga agaaaaagtt
    10321 acgctgatgt gcgaccagag cgatgcaata cctcgtttgg acatcaaaaa attcaactgg
    10381 tggagtgaag ctttacatgg gtatgccaac aacgatagtg taactgtgtt tcctgaacca
    10441 attggtatgg cagcctcatt caatgatgag ttgcttttta agatttatga tgctgtatca
    10501 gacgaaggac gtgccaagta tcaccaatgg ttgcgcaggg gcaatgaaaa taagcgtttt
    10561 ttgagccttt cggtatggac accgaatgta aatatatttc gtgatccgcg ttggggtcgt
    10621 ggccaggaga cctatggaga agacccttat cttacttccc gtatgggcgt tcaggtagtt
    10681 aaagggctac aggggcctgc tgatgctaag tataaaaaac tccttgcttg tgccaagcat
    10741 tatgcagtgc attcgggtcc tgaatggagc cgtcatcaac taaaccttaa taatgttgat
    10801 cctcgcgatt tgtatgaaac ctatttgcct gcatttaaag cattggtgca ggacgctgat
    10861 gtccgtcagg taatgtgtgc ttatcaacgt ttggatgatg aaccttgctg cggaaataca
    10921 aggcttttgc aacgtatttt gcgcgaagag tggggcttta agcatattgt agtttctgat
    10981 tgtggtgcta taaccgactt ttttaccagt cacaaagtgt catcaactcc tgtacatgct
    11041 gcatctaaag gggtattggc aggaacagat gttgaatgcg tgtgggaaaa ttacccgtac
    11101 aaaacccttc ccgatggagt taaacgaggg ttacttaaag aagaggatgt tgataaaagc
    11161 ttgatgtgtg tattggttgg tcggtttgaa ctgggtgatt ttgatgatga caagttagtg
    11221 ccttgggcgc aaatccctgc ttctgttctt aactgcgaaa agcataaaaa gcttgcttat
    11281 gaaatggcac agcagtcaat gacattgttg caaaataaaa agaacatcct gccattgtca
    11341 aaaacgtcag ggagaatagc tgttattggc cctaatgcaa atgataaagt aatgctgtgg
    11401 ggaaattata atggtacgcc aattgaaaca gttactatcc ttgatggtat aaaaacaaag
    11461 ctttcggata aaaaggtttt ttatgataaa ggttgtgacc ttgtagaaga taaagtaaca
    11521 gacagctatt ttggacaact atcgtttgag ggtaaaaaag gctttaaagt aacatattgg
    11581 aacaaccctg aaatgaaggg tgatgctgtt ataactcagt atatcaccga tccgataaaa
    11641 aaaacaactg cagggcagca cgaatttgct tcgggggtaa aacttgaagg tttttcggct
    11701 ttatttgaag ctgaatttgt gcccaaagta tctgaagagt tgatttttaa aggtggggca
    11761 accggttttt ttgaactggt tgtaaatggc gatactctca agagatacca caactggaga
    11821 actttacctt cacggatccc attgaaggtt gaagccggga aaaaatacaa tattaaaatt
    11881 agctatgccc agctcaacaa ttggcaggcc aatattgagc ttgactttgg caaagaagta
    11941 gatgttgact atactggact tatcaataaa cttaagggaa tagaaactgt tgtgtttgtt
    12001 ggaggcctct ctggtaatct cgaaggtgaa gaaatgcctg tgtcttatcc gggtttcaaa
    12061 gggggcgacc gtaccaatat tgagctgcct ttggtacaac gcaactgctt gaaagccttg
    12121 aaacaggctg gtaaaaaagt tgtttttgtc aattgttccg gttcggccat agcgttgacc
    12181 cctgaaaccg aaagctgtga tgcgattttg caggcatggt atgccggaga gttaggagga
    12241 atggctgttg ccgatgtctt atttggagat tacaacccct ccgggaagtt gcctattaca
    12301 ttttataaaa gctctgatca attaaaggat tttgaagact attcgatgaa aggccgcacg
    12361 taccgattta tggacgatgc attgttccct tttggctatg ggctgagcta taccacattc
    12421 tcgattggaa acgcaaagct taacaaaaca tctttaaata aaggtgattc acttgttctt
    12481 aatattcctg ttatcaatgc cggaaaaaga aacgggactg agattgtcca ggtatatgtt
    12541 cgcaaggtaa atgatacaga tggcccctta aaaacactga aaggctttaa aagattgaat
    12601 gtatctgccg ggaagactgc tcaggctgtt atttgcctgc catacaattc atttgagttt
    12661 tacgaccgta caactggcaa aatgactgtt gctgctggtg agtatgaagt tttctacgga
    12721 aacagctctg ataaaaagga tttgaaaact gtaaaaatta ctgttcaata aaaacttata
    12781 aaatatttta atcatgctta aacatacatt agtagcgcta ttatctgtat ttagcctgtt
    12841 tggtgttgcc cagcagccaa cggttattga agattttaaa ccatcaacgc tcaatcagcc
    12901 cgggaaagaa taccctatgg taaactcgca ggggtatgcc cgttttcgtg tcatagctcc
    12961 tgaagctcaa agtgttattg ttagccttgg attagggggc aaaggaggaa ctaagcttgt
    13021 caaaggggca gatagtgttt ggacagggac aacagaaggc cctatggacg aaggctttca
    13081 ctactatcat ttgactattg atggcggtat ctttaatgat cccggaactt tgaacttttt
    13141 tggttcgact cgctgggaaa gtggaattga aataccagcc catgataaag atttttatgc
    13201 attgaaagat gtacctcatg gtaatgtcca acaggtattg tttccatccc caagtaccaa
    13261 cagcgtgcgc agggctttta tttatacccc tcccggatat ggtaaagata aaaataaaag
    13321 gtatcctgtg ttgtacctgc aacatggatg gggcgaagat gaaacagcct ggagcaacca
    13381 gggacatgca aacctgatta tggacaacct cattgccgaa ggtaaggtta aacctttcat
    13441 tatagtgatg acgtacggaa tgaccaacga aatcaagttt ggaactttga aaagctttga
    13501 tataaaacca tttcagacag tacttgtaaa tgaactgata ccttatgtag atgctaactt
    13561 tctgacactt tcagacagaa acaatcgtgc tatggccgga ttgtctatgg gaggcatgga
    13621 aacgaaaatg ataacccttg ccaatcttga taaatttgct tatatcgggt tgtttagcgg
    13681 agggacattc tcaatggaag atgttaacaa tactcctggt tttaaagaaa aagtgaagct
    13741 ggctttcgtt agtttcggta gccgcgagtt agagcaccgc tggcctggtt ttggcggagg
    13801 cgatccaaaa gagaatgcag aggccctaaa acaagctggt ataaacagtg ttttctatgt
    13861 ttcacccgga acagcccacg aatttcttag ctggcgccgt agtttacgcg aaatggcccc
    13921 actattattc aaataaggat tgagagtatt aattttttgg taatgagccg aaggctttcc
    13981 agttttcaaa aacctggaaa gcctaagctg ctaaatcagc aattttgacc caagaccatt
    14041 tttttaaatt atacaaaaat caatgaatat taaacctatc catgctttgt tgacggtatt
    14101 acttgcaagt caaataagct ttgctcaaaa taccccttct gtcgtaaagg aagatttcag
    14161 accttcatcg gtcaatcagc ccgggaagca atatccgcaa gtaaactctg aaggccgggt
    14221 gcgtacaagc attactgcgc ctgaagccaa caaagttcaa cttgatattg gcggggtgaa
    14281 atatgacctg gttaaggacg caaatggtgt atggactggc gaatctgccc cgcaagatga
    14341 agggtttcat tattatcagc tcaatattga cggggcatct gttcctgatc cgggaaccaa
    14401 atactattat ggtgccggac gctggggtag cgggatagaa atacctgcgc cagaccagga
    14461 tatctatgct ttaaaagatg tccctcatgg cttggtaagc gaggtggttt atttctcaaa
    14521 aatcactcag gcatggcgtt gctgtattgt ttacactcct gccggatatg aaacaaacgc
    14581 caatgttcgc tatccggtgc tttatcttca gcatggaagt tttgaagatg agactggttg
    14641 gtcaagccag ggaaaagcta acctgatact cgacaacctt attgctgata aaaaagctgt
    14701 tccgatgatt attgtaatgg acaatggtta tgcttttaaa ccacaggaag gagaaaataa
    14761 gaaaccttta ttggtttttg aagaggtaat gatgcaagaa attatcccca tgatagattc
    14821 ccgctttagg actattgctg ataaaaggca ccgtgctatt gccggtttgt caatgggggc
    14881 caaccaaacc ttgcgcattg ccatgaacaa tcttgcagct ttttctgctt atggcggatt
    14941 tagtggcact gccaattatc caagtgccga tgaaattgac cctgtaacct ttcttgatgg
    15001 aaaatttgct gaaggtaagg ctgtaaatca acaattgcct gtcttttttc tcagtttagg
    15061 tacaaaagag cctaatccat ttccgggttc agtaggggcc tttcgcaaaa tgcttgacaa
    15121 acagggcatc aaatatagct tttacgagtc gcctggtaca gcccatgaat ggcagacctg
    15181 gcggagatct ttgtatcagt ttgcccagtt attgtttaaa tagattttca aaattttgaa
    15241 cagttgaagt ttatgaaatt tttagccatc aggaattact tagcttttac tattttgact
    15301 ttgttgcttt gttcgatggg cattgccaaa gctcagttga aagaaaactc agttgcggca
    15361 acaacaaaca ttgctcaaaa cctgtgtcct tgtattatgc ccgataacag tgtttttttt
    15421 caggtaaaag ctcccaacgc ccaaaaagta cagattgatt tggggaaaaa gtatgatatg
    15481 gtgaaagcta ctaatggcgt ttggtctgtt cgtacagagc ctgtggtacc tggttttcac
    15541 tactattttt tggtgattga tgatgtccct gttgctgacc ctgcaagcca atcgttctat
    15601 ggaacaggga agatgacgag tgcaattgat attcctgaaa aaggtataga tttttatgac
    15661 ataaaggatg tcccacatgg ttcaattagt tcaaaatact attattctaa agtagataat
    15721 tgctggaaga ggctttttgt ttatacccct cccggatatg accaaaatac caatatgaag
    15781 tatccggtaa tatatattca gcacggtggt ggggaagatg aaaccggttg ggcaatacag
    15841 ggcaaaaccg accttattct tgataactta attgccggta aaaaagctaa acctatgata
    15901 attgtaatgg ctaacggaaa ctcaagggca ggaattggag gctatacaag tgaagcaatg
    15961 gcaaggttta aaaaggagat gactcaaaat attgtcccgt ttgtagatga aaactacaga
    16021 acattggctg atgctaaaaa tcgtgctatt tgtggattat ctatgggagg tggacaatcg
    16081 ttttatgttg gacttcaaag ccttgaatat tttagttcgg ttgggatatt cagttcggga
    16141 atttttgggg gtattcgtaa tccttcaggc aaagagttta atgctgaaac ggaaataccc
    16201 ggtttgttgt caaaatcgga tgaattcaac gcaaagcttg atttgtttta tatatcctgt
    16261 ggagaacaag accccagaat tgaatttaca aagaaagcgg tggatgttat gaaaaaaaat
    16321 ggattagaag ttgaattcaa ttcttttcct ggtgatcatg aatggcaagt atggagaaaa
    16381 tccttacatg attttgcttc cagagttttt aaatagcatt atttaaatat aataacaaac
    16441 aaatacaatg aatatcagga aaatattatt tcacacttta agctttttgc tgataatatt
    16501 tccggcgctt gtaagagggc aacaggctaa tgtcaacctt gactataacc ctcaaaaaaa
    16561 caccgaaggc cttattccgt ttagcgcccc gttaaattcg cctgaggtac atgatgacca
    16621 gacggttact tttcggttaa aagccccaaa ggcgcaggaa gtaaaattgg caggtgtggc
    16681 tatcctcact gctcttggta agggaaaaga gatggtgccg tttaccaaag gcgatgatgg
    16741 agtttggaca ttaactgtgg gcccgcttaa acccgatatg tatgcatatc atttcaatgt
    16801 tgacggagtg cagataaccg acccttccaa tacttacgcg gcttttaccg ctatgccgcc
    16861 atatagccag ttaattgttc atggtagcgg gcctgcttat tacgatgcac aaaatgtccc
    16921 gcacgggaca gtaacccgtc atatttatca ttcagaagtt actaatggtg agcgcgaact
    16981 ttacgtttac actcctcccg gatataaacc tgataaaaaa tatccagtgt tgtaccttct
    17041 gggtggaagt ggagagcttc cttcaaactg ggtgtacgat ggcagggtca attttatcat
    17101 ggacaacttg ctggcagaag gaaaagcagt gccaatgatt atagttatcc ctaataatca
    17161 ggttgttcat cgcaatcatc cgaaacatgc cgaattgacc tttgatattt ttgaaaagga
    17221 acttagaaac catgttgttc ctttgattga tcaaaactac agtactatta aagaacctaa
    17281 agggcgtgct ttgtctggac tttcgatggg aggccgccac agtatgtata ttggctttcg
    17341 ttcgcttgat ttgtttgcca attttggtat tcttagcgct ggcgatgtcg aagccgaaaa
    17401 gtctttagct caatttttaa atgaccctaa agtaaataac aaagttgatt acctttttgt
    17461 cggacaaggc actgaagaag caaatggctt tatgggccaa cgctgcattg ctttgcatga
    17521 ggcattggcc agacataata ttgaacatga atattatgta ggcggatacg gtggccatga
    17581 ttgggctacc tggcgtcatc tcttgtatta taggtttctt cccaatcttt ggaaaaaaat
    17641 taacaatata aaaaaataat catgaagcaa ataaacttgt tagtggctgc atttgtcttg
    17701 tttggtattg tagcctgtaa aaaacaaatc accgggcagg tggaagtgga aggcgggatt
    17761 atccagggta tagttactga ggatttgacc atctttaaag gggtaccttt tgcagctccc
    17821 ccggttggcg atttgcgttg gaaagctcca cagcctgttg taaaatggga aggcgtaaaa
    17881 caaactaccg aatttgcccc ggctccaatg caaggtggta accctgtttc gggcaaaagt
    17941 gaagactgtc tgtatttgaa tatatggact ccggccaaat ctgtcgatga aaaattgcct
    18001 gttttggtat ggatttacgg cggaggtttc agttttggct ctacctcaga tccggtacat
    18061 aatggagaac atttggcacg aaaaggagtt gtagtcgtaa gcatagcata cagggtaggg
    18121 cagttgggtt ttttagctca tcccgagttg agtgccgaaa atcctgaaaa agtttcggga
    18181 aactatggtt tgcttgacca aatagccggt ttgcaatgga tacaacgcaa tattgctgct
    18241 tttggaggtg accccgaaaa agtgaccatc tttggggagt ctgccggagc tatctcggta
    18301 agtatgcttt gtgcttcgcc tttagcaaaa ggattgttta ggggggctat ttcgcaaagc
    18361 ggtggctctt ttggccctac aaggcctaca acttacccgg gcgagaatat gaaaaccctt
    18421 aaacaggctg aaaaagaagc tgaaagctat cttcaaatgg ccggggttaa aaccatcgct
    18481 gagttacgaa agatagaagc agataaattg cctgcaggat gggggatgcc cggagcatgg
    18541 cctattgttg acggctatgt aattcccgac gaccaatata aactttatga aagtggcaaa
    18601 tacaatgatg ttgcagtatt aattggttac aattctgatg aagggttgag ctttccgacc
    18661 ggacgcacac ctgaagagtt tgtctctaat gtaaaaatgc gttttgggcc ttttgccgac
    18721 agccttttac aagcttatcc gcttgaaaag aatgcaattg cccggagcgc ccgcaacctg
    18781 atgcgcgatg ctgcctttgg ctggcatacc tggagctggg caaggcttca gtctcaaaca
    18841 ggaaagtcaa aggttttcta ttactatttt gaccaacatc ctgtacgtcc cgaaggttcg
    18901 cctcaggccg accatggcac accacacgga gtggacgttc cttacgtatt tatgaattta
    18961 gatacagcca atcagggtgt taccaagtct gacattgaga tatcccagat tatgggtact
    19021 tattggacca attttgccaa atatggcgac ccaaatggag atggagttgc ccaatggcct
    19081 gcattttcgg ctaacaatcc tcaggttatg tatttaggcc ccaatacttt cataggtaaa
    19141 gtgcctgatg aaaaggcctt acaggtgttg gatggctatt ttgcatggag aaggtctcct
    19201 gaaggagtgg cctgggctaa ataagtgtta aacgttaaat aataatatag taaagtgaaa
    19261 aaaatcatac tgataatcac cgtaatgttg ggattaaacg caggtatttc tgcacaagat
    19321 cctaattttt atattttcct ctgttttgga cagtccaata tggagggtaa tgcaaaagtt
    19381 gaacctcagg atactattgt agatagtcgt tttcaggtga tggaaacggt taattgccct
    19441 aatttaaaaa gagataaagg gaaatggtat gctgctgtcc ctcctttatg ccgttgttac
    19501 acaggcttaa ctcctgctga ctattttggg cgcacgttgt tggttaatct ccccaaaaat
    19561 gttaggatag gggttattaa tgtagcggta ggtggttgta aaattgagct ttttgacaaa
    19621 gataactatc aaacctacgt agcttctgct cctgattggc tacagaatat ggtaaaagag
    19681 tacgatggca acccgtatgc ccgtttggtg gaaatggcca aactggctca aaaggatggc
    19741 gttatcaaag ggatattgct acaccagggc gaatccaata ccaacgattc gctttggcct
    19801 gcaaaagtta aaggcgtata tgataatctt ttaaaagacc ttaaccttac agctaattca
    19861 gtgcctttgt tggccggaga ggttgttgct gctgaccaaa aaggggcatg tgcaagtatg
    19921 aatgctatca tagcgcagtt gcccaaggtt attcccaact cttatgtaat accttcaaca
    19981 ggatgccctc agcgggggga taacctgcat ttcacagcag aaggctaccg aatgctgggc
    20041 aaacgttatg gagtaaaaat gctttcgtta ctgcttgata aagttcaata aagggttttt
    20101 aataaaaata aagaaaaatg aagaaattgt tattgtcttt tgcttttgtt ggtttgtgtg
    20161 gttttgttgg ggctcaaaag cctattgtac aaacatattt taccgccgac ccggcgccaa
    20221 tggtttacaa cgacacagtc tatttatata cctcgcatga tgaagattct acggtgaata
    20281 atttttttac aatgtacgat tggcgttgct attcctcgac cgatatggtt aactggacag
    20341 atcatggggc agtagcttcg ctgaaaaaca ttgcctggtt tgataaaaac aatggggcat
    20401 gggctcccca atgcattgcc cgtaatggca agttttactt atatgtacct attcatggtg
    20461 gtggaatatc agtattggta ggtgattcgc cgacaggccc ttttcgtgat gtgcttggta
    20521 agcgcctgat agatagcgag catatttgga acgatattga ccctaccata tttgttgatg
    20581 atgatggcca agcctattta tattggggaa atcctgtgct tcgctatgta aagctcaata
    20641 atgatatgat ttcttatgat aaaagcattg ggaaaaatgg cattgtttca ttagaaatga
    20701 ctggtgaagc ttttggagag gctgttgacc gtgatgggaa acctcgcaca aaatatgtcg
    20761 aaggcccttg gttttacaaa cgaaatggca tttactacat ggtttatgct gctgccggaa
    20821 tacccgaata tattgcatat tcaactgcaa catcgccggt aggcccttgg acttacaagg
    20881 gatttattat ggaaagggct ccaaagcttg cttttaccaa ccattctggt ataattgatt
    20941 ataaagggaa ctcttatttc ttctaccaca gcgaatcatt gactggtggt ggtggtttca
    21001 ggcgttcggt ttgtttggag cagtttaagt acaatgctga tggttctata cctcttattg
    21061 tgcctactgc agaaggcgtg aaagaaagct tgtcggcttt aaacccatat aaacgtgtag
    21121 aagcagaaac tatggcttgg tcagaaggct tgaaaacagc aaaagacaac agtaaaggta
    21181 tttatgttac taaaatcaac aatggtgatt atatcaaggt gagtagtgta gattttggaa
    21241 aaggggcaac aacatttgaa gcaagccttg cttcggctgc aaaaggcgga caaatcgaaa
    21301 ttcgcttgaa tagcaccgat ggtgaactgt tgggtacttt agacgtaaaa aatacaaagg
    21361 gagcgaataa ctgggctgtt caatcttgta aagtgaaggc tgcaaaaggt attcatgatt
    21421 tgtatttggt tttcaaaggc ggagaaggcg atttgttcaa cttcgactgg tggaaatttt
    21481 caaaataata gagatagaaa atacattgga ggtaaaaaat aaaatataga taaaatggga
    21541 cgcattggaa agttgatgat ggtagcaatc cttatgattg ctttaagtat ggcgggaacg
    21601 aatacttttt cagcccaaac cgtttggctc gatgagcttg atttgagtgc agccacgcaa
    21661 gggtggggcg ttccgatgaa aaatagatct attgacaata aaacgcttac cattgctggt
    21721 aaaacctttg aaaggggctt tgcaacacac tcggtaagct cattgttgat tttgcttgaa
    21781 ggaaaagccg tttctttttc tgccaaagtt ggtattgacg atgaagttaa agggcatgat
    21841 gctgctgccg aatttgaatt gtatggcgat ggtaaaaagt tgtggtcgag cgggcttatg
    21901 cgtgttggcg atactgccag ggtatgttct gtaaacctta caggtattaa gaaattcgag
    21961 ttggtagtag cagatggcgg caatggtaat tattacgacc atgccgactg ggttgatgcc
    22021 aggtttgaag ccaaaggtgt tacaaccttt aaaacataca accccgtagc cagtgagcct
    22081 tatattctca ctcccaaacc ttcggataaa cctcgcatca atagtgcaaa agtttttgga
    22141 gttcgcccgg ggtcgccgtt tcaatatctt gtggctgcta ccggcgaccg cccaatggtt
    22201 ttttcagcta agggtttacc tgaaggcctg aaaatagacc aaaatacagg tttgattacc
    22261 ggggtgctct ctaatgccgg gacttatatg gttacccttg aggcaaagaa tgcaaaaggc
    22321 agggccgaga aacagttgcg catcgagtgt ggcaaccgta tagcccttac tccgcctatg
    22381 ggatggaaca gttggaactg ttttgcccac gaggtatcgg ctgagaaagt aaaaaaagct
    22441 gctgaagcta tggtcaaaag cgggcttatc aatcacggct ggacttatat caatattgac
    22501 gatttctggg aaaaccatcg cgattccaaa gacccgtcgt tgcagggtaa atttcgtgat
    22561 gatgctggct atatcgttac taattcaagg tttggagata tgaaagccct ggctgattat
    22621 gttcattcac ttggtttaaa aatagggttg tattcaagtc ccgggccatg gacatgcgga
    22681 ggctgtgccg gaagctatgg ttttgaaaaa caggatgctg aaagctatgc caaatggggc
    22741 tttgattacc taaaatacga ctggtgcagc tatggcaatg taattaatgg cttgcccgac
    22801 aatgacccat acaaggtttc atcattgtct tacaatggag gtggcgagct tgccacagct
    22861 attaagcctt tcaaacttat gggcgaatat atcaggcaac agccgcgcga tattgtttac
    22921 agtttatgcc aatacggcat gtcagatgtt tggaaatggg gcgactctgt tgggggcaat
    22981 tgttggcgta ccactaacga tatcactgat acctgggtaa gtgtaaaaag tattgcttta
    23041 gatcaggaca agtctgctgc gtatgctaaa cccggcaact ggaacgaccc cgatatgctt
    23101 gtcgtcgggc atgtaggatg gggcaatcca catccaagca aactcaaacc tgatgagcaa
    23161 tatctccata tcagcctttg gagcctcttt tcggctccgt tgcttattgg ttgcgatatg
    23221 gaaaaacttg atgatttcac gctcaacctg cttactaacg acgaggtgat tgaaattaac
    23281 caagaccctc ttggcaaaca agctacttgc aaacagacaa tcggagagct caggatttat
    23341 gtcaaagagc ttgaagacgg aagttttgct gtaggtttct gcaattttgg acttgatatt
    23401 gctgagcttt catataaaga ttttagcaaa ataggggtta atggcaaatt taaagtgcgc
    23461 gatttgtgga gacaaaaaga tataagcact attgattctc aaacagggca actgcctgtt
    23521 aaggtgccag ttcatggagc tttgttgtat aagttcactc cccaaaaaag ataatgggtc
    23581 gaaatagttg gaggaccttc cagctttcaa aaacctcgaa ggtttaagca gcaaagtcaa
    23641 taactttaac ccaagaccaa aaaaaagaat aacacacatg cttaaataac tataaataac
    23701 aactttatga gacaaactat tatttctaaa cacacaaatg ggcttgaaca taagtgttca
    23761 tgggtcaagt cttctttttt cattatggta tttgcgcttt ttagcctttc taccatattg
    23821 aatgcacaga tcgaaaattt tcaggttggt tcaacaaccc gcaaaatgct tgtttatgct
    23881 ccatctacta ttgttccggg gcgcccgttg cttatctcaa tgcacgggat gaaccaggat
    23941 atcaactatc aaaaaggcca ggcacagtgg gagacgattg caaagaaaga aaactttgta
    24001 gtggtttatc ctggcggtga aggcaattct tgggatttgg ccggggataa agacattaaa
    24061 ttcatcctgg ccattataga cgaaatggtt aaacgttaca gcattgaccg cgacagggtt
    24121 tatctttcag gtttttcaat ggggggtatg atgacttatt atgctgcaac taaaatagct
    24181 gacaaaattg ctgcttttgc cccggtgagt ggttataata tgggaggccc caatacgaag
    24241 agctcaaggc ctatacccct tattcatgtg catggtactg ccgatgatgt agttgcatat
    24301 agcggggttg ctgccagtat gaatgcctgg gtcgccagaa atggttgtcc aacaacccct
    24361 gtggttacca agccataccc ggtgggtaaa tctacttctg atgaaaaaaa atattggggg
    24421 cccgggattg attcggtaga agttgtacta attagtctta gtggcaaagg acactggcat
    24481 tcgatagaag caagcggtgt taataccagt caggaaatat gggatttttg caaaaagttt
    24541 gcccttggtt ttggagtccc taaatttaaa gctgcaacag tatcagatgt taatccaaaa
    24601 caaatccagg ttactttttc gaaaccttta aaagccttaa ataaatttga aggtttttca
    24661 gtgaaggttg acgggcagga tgctgccatc gatacagtgt tgtttaccga ttctctccac
    24721 ttagcaataa accttgttgc taatatttta aaaaccaatg aaataacttt atcttacaat
    24781 gggggcaacg tggtttctgt gtatgaaaaa cctttggtaa ctattaaaga tactgcagta
    24841 gataatcaac ttttaggttc tgcccctaaa ttggttcagc tttttgtaaa cgaaaaaggc
    24901 gatagcctta ttgctaagtt taccaaaaaa atgcttttgc ctgctgatct ttcatcaatt
    24961 gtgttgaagg ccaaatacaa cggcgaaaag agcgtttcat tgatcaattg ttctttttta
    25021 aacaatgatt ctaccacgtt ggttttccct cttgcagata cagtttatgc cgattacaaa
    25081 ttgaggctta gttataccgg caatgttgtt gcatcttcag atagcggaat gtttaaaaat
    25141 gatacagctt atgttataat taataagtct cgtggtttgc ctgctcatat tgtttctgca
    25201 attattgatg atagcgcaat agccatttca ttagagttta acaagcctat gtcgatgaaa
    25261 gatgcccaaa tttcgcagct tttgttgaaa gtaaacggga aagtaactaa aattaaagaa
    25321 gtattcaacg taaacaatac catcaggctt aatttgttta acagcttgta ttatcgcgat
    25381 tctattgtga taacatatac accgggtact attacagctg cagatagcgg atatttagag
    25441 gcttttaccg attttgtagt tgaaaatcca atagcagcac caacttatac agcaatacct
    25501 ggcaaaattg aagctgaaaa ttatgctatg caatttggta ctgataccga aactacaagc
    25561 gatgcaggtg gtggattaaa tgttggttgg attgatgccg gcgattggat ggtttatgcc
    25621 attgaaaaca acacagaaga tagtgtttac cagattaagt tccgtttggc cacccaggct
    25681 tcgggttcaa aatttgaata tttcattgat gatgtgaaaa ttggacaaat agatgttcca
    25741 aatactggcg catggcaaaa atttacttct gtaataaaag acattattgt ccctaaagga
    25801 aagcattact ttaaaatagt tgatgttact ggtaatttta atatcaacta ttttgtaata
    25861 cagaaagatt ttgtaagtgt aagccaaaca aataaagatg atattagcat tttcccaaat
    25921 cctgcttctg ataaaatagt gattaaatct gctggttttc agtatgataa aattgaaatt
    25981 tctgatatga tgggcagaac agtgttggtc aaatctttga cttatgcacc tgagctcgtt
    26041 ttgccactca atcttaacaa gggtacttat ttcttgaaga ttagcaatag caaatcttca
    26101 tttactcaaa aagttaatgt ggtgaaataa tttaaaaact tgttaatcta agtctatggg
    26161 gcgaatcagt ggatgaaaaa tgaatattat tggttcattc aggcatcaac tgtttcggcc
    26221 ccatagtttt taaaaacact tttttgaaac gaaagggctg ttttatttga aagacaatta
    26281 gttgaaacag atatgttaaa aagtataaac aatatcaaac ataaggatat ggctgagtgc
    26341 gtaaaacgta tgaattcttc agtaaaaagg tggatagcgt taagttgttt aatctcttta
    26401 accatgctgg ccttttctca acctgtaaag gttgtttcgc ccaatcaaaa aaatattatt
    26461 gagttgttca accatcaaga tggaaatgaa aaccgctggt ttttgaaaat atcctatagt
    26521 cgcgcgggaa gcaatgttga ggctgtttcc cgggtggata ttggactgtt gcgcaatgat
    26581 caggactttt caaataacct aaagtttata aaagcctcta agcctttgat tattgatgaa
    26641 caatattcga tgctgcacgg aaagaagcaa aaatgcacta actcggccaa cgaggttgtt
    26701 gtatattttg aaaatagcaa caaagaaaag atgaacctca ttcttagggc ttataatgat
    26761 ggggttactt tccgatatga gtttcccggc aaaggaggct cttatgtgat aaaagacgaa
    26821 cttacttctt accatatcaa ttctgcctac aaacgttgga tacagatgtt cgacctatcc
    26881 aacgaatggc tttttaaata ttataaaaat gatagtgtgc aggccatctg gggctatccg
    26941 gcgcttttca actcggccga cagcgcttgc tggtttctgt tgcacgaagc tgacgtgaac
    27001 cgaagttact gtgcatcgcg gcttaacaat aaggctgata aacaggctta caagattact
    27061 tttcccgaag attgggaagg taatggcgaa gtgctgccca tcattactct tccatggaaa
    27121 tcgccctgga gggtagttat catgggctct ttggcagata ttactgcatc aaccctggtg
    27181 gacgatgtat gcccttcgtc ggtcattgtt aataccgatt ggattaaacc tggtattgcc
    27241 tcgtggaact attggtcaaa taaccacggg accaaggatt ataagacagt gtgcgaattt
    27301 gccgatttgg cggctgctat gggctggcct tacacgctcc tcgactggga atgggaccag
    27361 atgggcaatg gcgggaacct tgaagatgct gtaaaatata tttattcaaa aggcgtaaaa
    27421 ccattaattt ggtacaattc gggggccttt aagtggatta ctgccacccc tgtggacagg
    27481 atgctcacgc atgaaaaacg tatggaagag tttgcaaagc tcaaaaagct tgggatagta
    27541 ggtgtaaaaa tcgatttttt cctgaacgaa aagcaggaaa tgatgaagta ttatcttgat
    27601 atcctcgatg atgcggcaaa gttcaatatt atggtttatt ttcacgggtg ccttgtcccg
    27661 cgtggctggg ccaggacata tccacacctg atgacttatg agggcatacg cggagccgag
    27721 tggtacaaca atggccccga atttacaagc gctgccccaa gtcataactc tatcatccct
    27781 tttacccgaa atgttgtagg ttcaatggat tatacccctg taacatttac caattcacag
    27841 tttccgcata ttacctcgta tgggcacgaa ctggctttga gcgtagtgtt tgagtcgggc
    27901 atacaacaca tggctgacag gccttcgggt tattatagtt tgcccgatgc cgctaaaaca
    27961 ttcctgatga acgtgcccaa cacctgggat gatatcaaac tgttggatgg atatcctggg
    28021 catgaggtta ttatggccag aaataaaggc agtcagtggt atataggtgg tattaatggc
    28081 gaaaacgtag agaaaaacaa aatactgaaa tttgactttt tgccggaagg tgctaaatat
    28141 aaacttacgc taatagctga tggtgagcat gataaggaat tgaaagcgca atatatggtg
    28201 gtcgaaaaaa caagtttagt ggatgttaaa atgctccgca gaggcgggtt tgttgcacaa
    28261 ttaataccaa ttcaataatg aaagtgttga aatattttat cttgaaggcc ctgttggttt
    28321 taataagcct gttcccggca atggtttatg gtcaggcata taaatctcat gcaattcaaa
    28381 aagaccgtct ttcggttcaa cttacagaag gggtattgga tataatccct cttgctgatg
    28441 gcgctgtacg ggtacaatac aagaaagaac ttttaaaaga actacctgaa ctgattttta
    28501 ccaatgcgtc ttctgctgtt aagtttaagg tttcggatgc cgagtcggcg ttgatttttt
    28561 caactgataa aatgattgta aaacttcata aagctaacgg tagtttggct tttagcgata
    28621 aatcgggaaa agtattttta agcgaaaaag aaaacagccg ttggttggtg cccgattcgg
    28681 ttcatggcga gccttgttat aaggcagagc aaagctttgt ttcgccttcc ggcgaagcgc
    28741 tctttggctt aggccagttt caggatgggc attacaattt gcgtggggta acaaggcgtt
    28801 tgatacaagt caattcgcaa atagctttgc cttttatata ttcgggcaag gggtatggtt
    28861 tgttatggca tcaatacggg cttaccgatt ttaatcctgc cgacaatttc atttctcttg
    28921 aaaaacaaga agcttctgct tcggccaata atcaggtagc tgaagttact acaacgtctg
    28981 ggatgcagaa agtgtcgcaa gatcaaacag cctataaagg caaatttaca gtccctgtag
    29041 atggcgaata ttctattttt cttgatttgg gcgatatggg caatcgccag tttgttgtca
    29101 ttgacgggat tccgtgtatt gaccagagta acctctggct cccgccatca ataagtgctt
    29161 tggtaaaatt aaaagccgga gagcatcacg ttcagctggt ttgcaaatcg tccaacaacc
    29221 ctgagctttc atggaaaaga aacgataatc ttactacatt ccgttcgcct aatgcaacaa
    29281 tgcttgatta tgttgtgttt tatgggcctt cggccgatag cgtaatttct gcttaccgca
    29341 acctttcggg aaacgtaccc atgtttccgc aatgggctta tggcttttgg caatgtcgcg
    29401 aacgctacac ctcgggttct catttggtcg cgaccgtcaa ggaattcaga aaaaggaact
    29461 tgccaatgga tgtcattgtt caggattggc aatactgggg caataaaggc tggggtgtac
    29521 ctgtgtttga cgatgtcaac tacgccaatc cggctggttt tattaaagaa attcacgacc
    29581 agcatgcaca tttcaatatt tccatctggt cgaaccccga taggaactca acgcttggaa
    29641 aagactatgt agcaaagaac ttgtatgttc aaaacagcaa gtggcttgac tatttcaacc
    29701 ctgtaacccg cgaaacctat tggaaaagct tgaatgacaa tctttttaca cacggtgttg
    29761 attcttggtg gatggatgcc accgaacccg agaacgatgc gttgaaagga gcgaagacgt
    29821 tctatggact tggagatttt taccgtctta cctatcctat gtttgtcagc caggctgtgt
    29881 atgaaggaca gcgcaagctt acatccgaaa agcgtgtttg tatccttacc cgctcggcat
    29941 tccttggaca gcagcgatac ggcgttatca actggtcggg cgacattggc agcgactggg
    30001 atacttaccg tcggcagatt gttgccggat tgaattatac cattaccggt ttcccttact
    30061 ggacaacgga tataggcggc ttttttcgtc ctggcaatgg acaatatacc gatgaaaaat
    30121 accacgaagt ccttacacgt tggtttcaat ggggcgcttt aaacccaata ttccgcattc
    30181 atggttttat gagcgaaaca gagccatgga aatttggaag ccaggtagaa aacaacatgc
    30241 gcaaaatgct cgatttgcgt tatcgcttat tgccttatat atactcggag gcatggcaag
    30301 ttagccacaa cggttcaaca ttgatgcggc ctttggtgat ggattttaat ggagatactg
    30361 ttgctgttgg acaaccttat gagtttatgt ttggcaaagc gtttttggtg gctcctgtaa
    30421 ccgaacccaa tttgaaagaa caggcagttt atttgcctaa aactgctaca tggtatgact
    30481 tctggacagg taaacgccta aatggcggcc agacagttaa aacagaaatc ccgttagata
    30541 aggtgccatt gtttgtcaag gctggcgcta ttgttccaat gggtaagttt attcaatatg
    30601 ccgggcaaaa aagtgccgat accctggaga tacgtgttta tgccggtgcc gatggcacat
    30661 ttggattgta cgaagacgaa ggcgataatt acaactatga aaaaggcggt tattcaacca
    30721 tttcatttga atggaatgag aaaaagcaaa ctttaacgat aggccaaaga tatggcgatt
    30781 tttccggttg tttgaaaaac cgggtgttta acgttgtgct ggtaaacgaa aacaatggaa
    30841 caggcatatt gtctggagtt gcatcaaagc aggtgctgta ttcaggaaag aaaataaatt
    30901 taaaactata aaatattgat aattagatgg gtaataagtt aaaaatttta gttgcattga
    30961 catgtttagc tgccgttgct attagtccgg caagaggtgc agataaaaac caaaagttga
    31021 aagatgcgtt tgcggggaaa ttttttatcg ggacagcgat gaatgctgaa cagattctcg
    31081 aaaaagacac tgctgctgtt cgccttatca aagaaaattt tggcgctatt gttgccgaaa
    31141 attgtatgaa agctggtcct attcaaccaa aagaaggaga gtttgacttt tcgttggccg
    31201 acaaatttgt cgaattcggc gagaaaaaca ataaatttat taccgggcat tgccttgtat
    31261 ggcattcgca ggctccaagg tggtttttta aagacagcct gggcaatgat gtttctcgtg
    31321 aggtgcttat tgaacggatg aaaaagcata tttacactgt cgtaggccgc tacaaagggc
    31381 gcgttaaagg atgggatgtg gtaaacgaag ctattgacga cagagatggc tcgcttcgta
    31441 aaagcaaata ttaccagatt attggtgaag actttatcaa gctggcattt cagtttgccc
    31501 atgaagccga ccctcaggct gaattgtatt acaacgacta ttctctttcc gatcctaaga
    31561 aacgaaaagg ggcaattgaa atagtcaaaa agctcaaaga acaaggcgtg aggattgatg
    31621 ctgttggaga gcagtgccat gtaggccttg attaccctac cattgatgaa ttggaaaaaa
    31681 ccattaccga atttgctgct ttgggcgtga aagtgatgat taccgagatg gatgtggttg
    31741 tattgcctat ggattggagc cttggcgctg atgtgtcggc tcgtgttgaa tacaagaaaa
    31801 aatcagattt atacgttaat ggcttgccag actccgttaa tactgttttt gaaaaaaggt
    31861 atttagacct gtttaagctc ttcctgaaac acaaagatgt tattacccgc gtgacgctgt
    31921 ggggggttac tgatgctaat tcgtggaaaa ataattggcc aatccccgga aggacagatt
    31981 atcctttgtt gtttgacagg aaatatcagg tcaaacctgt agtggggaaa attattagtg
    32041 aagctttgaa gaataactaa aatctgtttt tgaaatcttt ttcaattgta aacgtaaacg
    32101 accttgaaaa ttgaataatg ttttgtaact taataagata gtaataatta aaaataatga
    32161 atatgaaaaa ggagaattta aaaatgagga aaaacagaca acttatgctt ttgtctgttt
    32221 ttgtagcgtt attttctttt gttgccttga gtggcaaaga taaaggcaaa gtagccaaaa
    32281 atgccccggt gttcagcaat tttgtgtacc agggcaacga caaggtttat accgacaacc
    32341 cgctgaaagc tgatgagttt tacaacccca tccttcaagg atgctacccc gaccctagtt
    32401 ttacgcgcaa aggcgatgat tattatcttg tatgttcatc atttgctatg tttccagggg
    32461 tacctatttt tcattccaag gatttggtca actggacaca aataggccat gtactcgacc
    32521 gtacttcaca gctaaaggtt gaggattgtg gtatcagcgc cggcgtttat gccccccaga
    32581 tattgtacaa ccccaacaac gatatgtttt atatgattac cacccagttt tcgggaggtt
    32641 ttggcaatat tgtagtaaaa accaaagacc cattgaaagg ttggagcgac cccattaagc
    32701 ttgattttaa tggcattgac ccatcactgt tctttgatga taatggcaag gggtatgttg
    32761 tgcataatga tgccccggat ccaggtaagg agctttatag cggccatcgt gttatcaaaa
    32821 tatgggaata tgatgtgcaa aatgacaaag tgatacccgg aacagatgta attattgttg
    32881 atggcggcgt tgatatcaac aaaaagccta tctggattga agctcctcat atttacaaaa
    32941 agaatggcaa gtattacttg atgtgcgcag aaggaggcac aggtggatgg cacagcgaag
    33001 tgattttcgt tagcgataat cctaaaggcc cttacaaacc tgccccaagc aacccgatac
    33061 ttacccaaag gtattttcct aaagaccgcg tgaataaagt ggactgggca ggccatgccg
    33121 acttggtttt aggcccagac aataaatatt acggggtgtt cctggccatc cgccccaatg
    33181 aggctgaccg tgttaatata ggccgcgaaa cttttatttt gccggttgac tggtctggag
    33241 agtttcctgt ttttgaaaat ggcctggtgc ctattgaacc aaagctgaaa atgcctaaag
    33301 gcgttgaaaa caaaactggt aaagatggat ttttccctaa cggaaatttc acttttaccg
    33361 acaattttac aactgaaaag ctcgattatc gttggattgg cttacgaggc ccccgcgaaa
    33421 actttatttc ggttactaaa aaaggcttac agattacgcc atttgctacc aacattaagg
    33481 aagtaaaacc aacatcaaca cttttcttac gccagcagca caacaacttt tcgtacgcgg
    33541 taacactcga ctataagcct ggttcagaaa aagaccttgc cgggataact gctttgcaga
    33601 gcgaaaactt caattacgtg tttggcatca ccaaaaaagg atcagattat tacattttgt
    33661 tggaaagaaa tgagaaacaa aggaggagag atgccgcttt ggagtcgaaa atcatgggca
    33721 gtgccaaaat agacctcaaa agccctgtaa ggttgaaagt ggaagccaaa ggcgataatt
    33781 acgagttcag ctattcgctc gatggcacta catttcagac gttgggtggc actgtttctg
    33841 gcgacatcct ttcgaccaat gtagccggag gctttacagg ctgccttata ggcttgtatg
    33901 ctacatcggc caatgatgct aagcctgagt aaataatctt tgcaggcatg atgatgaaag
    33961 ccattttgat aagattaaat tagtgtagaa atgaaaaagt atataattgc tataatcggt
    34021 gtatcgttgt ttttgagcaa catcaatgct caggataaac tttatcctaa tacttttccg
    34081 ctgggagatg tggttttact agatggccca tttaaacatg cccgcgattt gaacattaaa
    34141 accctcttgc aatatgatgt tgacaggtta ttggcccctt accgcaaaga agctggtttg
    34201 ccagaaaaag caaagagtta tcccaattgg gaagggctcg atggccatgt tggcgggcat
    34261 tatttatcgg caatggctat aaattatgcc gaaacaggta atactgagtg taaaaagcgt
    34321 atggattata tgattgctga gctcaaagct tgtcaggatg ctaatggcct gaaatatccc
    34381 gattggggtg ttgggtatct tggtggtgta cccaacagcg ctgccctttg gtcaaaaatc
    34441 aaagctggag aggttggtgc aatctggtct gcatgggccc cattttataa tatacataag
    34501 atgtatgcag gtttgcgcga tgcatggtta tatactggca atgaagaggc aaaaaacatc
    34561 tttctgaaat tttgcgactg gggaataacc cttattgccg gtttggacga caagcaaatg
    34621 gaagatatgc tggccaatga acatggaggc atgaacgaaa tgtacgctga tgcttaccag
    34681 atgacaggag ataagaagta tttagaggca gcaaagcgat attcacataa ggcattttta
    34741 gaacccttgt cgcaagggat agatatgctg gataataaac atgccaatac tcaaatacct
    34801 aaatttattg gttttgaacg tatcttccaa cttagtggcg atgacaaata tggaaaagct
    34861 ggcagctttt tctgggaaac ggttacaacc aatcgctcgc tggcatttgg cggaaacagc
    34921 cgcagggagc atttcccaag tcctgcttcg tgtatcgatt ttgttagtga tattgatggg
    34981 cctgaatcct gcaattcata caatatgcta aagctaactg aagatttgtt cagggtgcaa
    35041 ccttccgcta agtacgccga ttattacgaa cgtacggtat ttaaccatat tttatcatca
    35101 caacatcctg aacatggcgg atatgtttat tttaccccgg cccgtccgcg tcattacagg
    35161 gtttattctg ctcctaacca ggctatgtgg tgttgtgtcg ggacaggtat ggaaaaccat
    35221 ggcaaataca accagtttat ctattcccat tcacacgatt cattgttttt gaaccttttt
    35281 attgcttcgg aattaaattg gaaagagaaa aagataaaaa tcaggcagga gacaaacttc
    35341 ccgaatgaag aaaaaacaaa actttctgtt gttgatggca cagccagttt caagctgatg
    35401 ctgaggtatc ctgcctgggt aaataaaaac agcttgaaaa ttaaggtcaa cggtaaagtg
    35461 ttttcttaca acgcccttcc ttcttcttat gtctgcattg acaggaaatg gaacaaagga
    35521 gatgttgttg aggttacatt accaatgcac aatactattg aacatttgcc taatgtaccc
    35581 aattacattg cttttatgca tgggccaatt ttgttatgcg ccaaaactgg tacagaagat
    35641 ttgaaaggcc tgatagctga tgatgggcga tggggacaat tggcagttgg agaaaagttg
    35701 cctgttgata aggccccaat tcttatcgag gatgatattc agcacattgc tgaaaagctt
    35761 gttccgataa aggacaaacc attgaatttt agcctgaatg taaaaatgat aaaccccatt
    35821 caggtgcagt ttcagccttt ttatcaaatt cacgatgccc ggtatatgat gtactggctg
    35881 gctttgacaa ataatggcta caagtcatac cttgactcgc tttcaaatat tgaaaaagaa
    35941 aagattgccc ttgaggaccg tacaatagac aaagtggctc ccggtgaaca acaacctgag
    36001 gtagatcatg ctatacaaaa ggaaaactct ggaactggca ataccaatgg cgtatttttt
    36061 cgcgatgcac gtgataatgg atattttagc tatgaattgg caacaaattc agaatccaac
    36121 cttagtctgg ttgttcgata ctggggagcc gaatggggca accgaaaatt tgatatttac
    36181 attgataatg aaaagttgtt gacagaagac aataccggtc gttggaacca gtctatgttt
    36241 aaagacgttg tttatactat acctgatgct atgttgagtg gtaaaaagtt tgtcagggtt
    36301 aagtttcagg caattaatgg taatgcggcc ggcggtgtgt atggcattcg tttggtaagg
    36361 aataagtaaa gtagccatta atctaacgat ttttaaaatg aagacattta aaatattggc
    36421 attattggtt tttgtagtca tttttgcgca aaaaatcaat gcccagaatg taaatactga
    36481 taagaaagac agtctttata aggattttaa gactgaattt gtatgggaag caaaagtgaa
    36541 aattgccggc atgataaatg taggccaaag taaacgtggc gaacgtcgcg ttatacctat
    36601 taccggtggg acatttaaag gcccgaagat taaaggcgaa gtgctgcctg gcggagaaga
    36661 ttggcaattg gttcgccccg atggcgatac cgagctttac gcacgttatc tgctgaaaac
    36721 cgatgatgga tatgttattc aggtgattaa tcgcgcgtta atccatgctg gtaaagagtt
    36781 ttattgcaaa tcagtattgg acttagaagc tccaacaaac agcccttacg agtatctcaa
    36841 tcatgccatc tttattggta ccctgaatgt ccctcctttg aaacaaggcg aagaacctta
    36901 tgttattatc ggggtttaca agttgcttta aactttaaaa tatgtttaaa atgaagatat
    36961 ttctttcaat actggctatt tgttcttttg ccttaataaa agtaaatgcc cagtcgtgga
    37021 ctgccgataa tggcaatggg acttactcta atccgctgtt tttcgatgaa ttttctgacc
    37081 cggatattat cagggttggc gaagattttt atctggcagg gacaaccatg cacgccatgc
    37141 ccggtttggt ggtgttgcat tcaaaagacc ttgtcaactg ggagtttatg agctattgtc
    37201 tcgattcttt aaatctgggt aaggaattta accttgaaga tggtaaagag gcttatggac
    37261 aaggtatttg ggccccatgt atccgctacc acaacaatat gttttacata ttcaccaatg
    37321 tcaacgggca cggtatgcag gtatttattt ccaaagaccc taaaggccct tgggttcata
    37381 aacctttgaa tacaaaaatt tatgatttgt cagtgctttt tgatgacgat gggaaaattt
    37441 atgccgtgta taactacaat gaagtcagga tggtagaact aaaacctgat atgagcggca
    37501 ttgtagaggg tactgaaaaa gtcattatcc ctgcaggaaa caatatgggg gaagggcatc
    37561 atatctataa aattaaagga aaatattata tcatcagcgc caattatgct cctgtaggcc
    37621 gtatgcaatg tgcccgtgcc gacaagcctt acgggcctta cgaaacggct gttattagtg
    37681 ctgaagaaac tatgggcttt aaaagaggct gggtgacctg gagtgtgggg cttggcaaac
    37741 ctgtgcccga acctggtttc aagtttaaac tcgacgagcc tggtgaaaac gactttggcg
    37801 ccgttccgct tcatcagggc ggtattgttg atttgcccaa tggcgattgg tggggctttt
    37861 ccatgatgga cttcatgtcg gtgggccgaa caacctgttt gtcgcctgtt acatggcatg
    37921 acggctggcc atattttggc ctgcccggaa accttggccg ttcgccacgt acctgggtga
    37981 aacccaatgt tgctgcggtt acctcccctt cggctcctta tcagagaaat gacgatttct
    38041 ctgggccaaa gctgcagcct gtgtggcagt ggaaccataa tccggttttg cgaaaatggg
    38101 aactcaataa gaaaaaaggt tcgttgcggc tgcatacttt gcctgccaaa gattttttgt
    38161 gggcaaaaaa cacgcttact caacgtgttg taggccctga gtcatatgct acggttgttt
    38221 tggatgctac ctttcttaag gttggcgata ttgccgggtt ggcttatttg aatatgcctt
    38281 atgcctgcct aggggtagct cgcactaaag a
//
LOCUS       DAOSPF010000033        38229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1111_length_38229_cov_6.174805, whole genome shotgun sequence.
ACCESSION   DAOSPF010000033 DAOSPF010000000
VERSION     DAOSPF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_38229_cov_6.174805"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1738)
                     /locus_tag="PK252_08095"
     CDS             complement(20..1738)
                     /locus_tag="PK252_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT14709.1"
                     /translation="MKYFIAIITGFILFGNYAQALDSPDPNFHIYICFGQSNMEGNAA
                     IAAADTIGVSERFKVMTVSAGDFLHNGRSAGNWYTAKPPLCRWDNGLTPADYFGRVLV
                     DSLPDSIKVGVIVVAMGGSGIDAFDKDNYTQYYQNADAWQKGLMDIYGGNPYAKIIEM
                     AKMAQKKGVIKGILLHQGESNNGQTDWPEKVKKIYNNMLADLNIEANSIPLLAGELLG
                     KDEGGICWGMNGIIATLPYYIPNAYAISSKGCAGNGKDGLHFSTEGARELGRHYGLQM
                     LSLLKTYNTVEGNTVDSLSVEKNSYTLLTGSIQRLSLTAIYADGHTLDVANKAGYSVS
                     NPDVVKMTNGYIEVVRDGAAVITASFKGSQGTMKQVDFKINATTFPLTNELLNPNIFS
                     NGSFDETTKTLKTGQWGFGGWQYSKGVDLSGYKYLVAKLGSANKADVEFKLFDGNNYW
                     GASTSGKFGNSRQVVVNLKNVKKGDGSTLEPSHIYIAGFWSNGSNAFVIDTVFLSNSQ
                     EFDPTGINDMPENNSELEMVDVYSVLGVKLRSKIIRKHAIEGLPKGVYMVGNKNGYVK
                     VIQNVN"
     gene            complement(2004..5168)
                     /locus_tag="PK252_08100"
     CDS             complement(2004..5168)
                     /locus_tag="PK252_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT14710.1"
                     /translation="MRKYLLLAFTVGFLMKSSEILAQADLKPGVDYPAEIENPELLGI
                     NKEPYHATLMPYANLQEALQAKRLASGLCKSLNGNWKFNWVPSPEQRPVDFYKPGFDV
                     SSWAEIPVPSNWEIHGYGTPFYRNFGYTIKKDFPRIMSTPESWFTAYKERNPVGSYRR
                     DFEVPAEWAGRRIFITFEGVDCAFFLWINGEKVGYSVNSRNAAEFDLTKYLKPGSNTI
                     AVEVYQYSSGTWLEDQDMWRLHGIFRNVYLWSAPQVHIRDFFVKQDLDKDYKDASVEV
                     VAKIKNYGDKGAKAQTLTATLYDKEGREVVKGNATAAAIASKQEQQVQIKFPVSNPAK
                     WTAETPNLYTLVLASTEGEILSTKVGFRKIEIKGRVFTVNGVPIKLKGVNRHEHWSDV
                     GHAITEEQMIRDLEVIKQGNCNHVRTSHYCDNPRWYELCDEWGIWLVAEANCESHGYD
                     GRFDEEPTMKAAIIDRNVANVENFKNHPSVIIWSLGNECGGVGSNFVAAMNAIKAIDS
                     SRPVHYERFGAGSRNPADLDGRMYGTAEDYVNMVKYWGLTKPLYICEFVHAMFNSMGS
                     LTDYSEAFDNNPEILGGAIWEFQDQALWNKRDPNHPILAYGGGFGEAPNDHYFIHKGV
                     VSFDRATGGTRVKPHFPEMKKAFQWIDISLVDPANGEIQIKNKYQFISLDGFDASWSL
                     TENGKEIDKGSLEIRNHWGRTIAIIPYKIENPKAGAEYFVRVSFTQKTKTLWADKGYE
                     VAWEQFKLPIKTLPVVEAKVTQPVKLIQDAQQVKVSGSGFALTFDKKTGFLTQIEKNG
                     KNILTSDGAPKLHLYRAMHQTDDWWVDREWSKYGVKDLKYSVVDMKTEIIDNTAAKVT
                     ATIKAEGKEGFAAYHTAVYTIKGDGTVKVDNKVQFTGFRINLARIGVRMLLDKKYDRL
                     NYFGRGPVENYADRKSAQDVGLYELEVNNQYEYEKPMEYGNREEMRWVKFNGADMPLF
                     EVKADEKLMQFSALPYTDEQMNPVEYKIDLPASTATVFCLSAKTLGVGSASCGPRPLE
                     KYQVKSDDTDFSYVLKLGDEKAEK"
     gene            complement(5210..6616)
                     /locus_tag="PK252_08105"
     CDS             complement(5210..6616)
                     /locus_tag="PK252_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT14711.1"
                     /translation="MTNLFKKLGSLAIAFSSFASYCFADNPIIQTKYTADPAPLVYHD
                     TVFLYTSHDEDGAFGFQMENWMLYTSTDMVNWTDRGIIAGVKEPYRTFAWADGHSAWA
                     PQCIERKGKFYLYCPTIYQGKMAIGVAVSGSPYGPFIDVLGKPLIFRSNPGDYDPTVF
                     IDEDGQAYLYWGGNGPCYYAKLNEDMVSLKGEIQVASIDFTGTPAEASYTEGPWLWKK
                     NKYYYLAWASRCCPEGIGYAMSDSPVGPWKCKGTIMDPDVRSSGNHPGVIEYKGKTYV
                     FGFNYNVLKRTMSKHYERRSICVEEIKYNADGTIQKHPWWSAKGVANVGTLNPFVKNE
                     AETMSFSEGLKTEFATEWERNVPWDKGKKIADRLYVTSIHNGDYIKVQSVDFAEGAKT
                     VEVSVASLYGGRIEFHIDKIDGPVVADINIVSSGEGDLWNTAKASVKDVKGIHDLYIV
                     FKGEKDLFNFDWWQFSNK"
     gene            complement(6694..7071)
                     /locus_tag="PK252_08110"
     CDS             complement(6694..7071)
                     /locus_tag="PK252_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14712.1"
                     /translation="MDNRLSFDSFINLELPKWFIYILLIFVFLWAFTAVMDFLRKKSG
                     IEAQSNSAHFDMIPFLIHFFKYSGIGMLWSCLFILLLQLVERAIRIDFSGLSVLLFCG
                     ILVLYISKALRVYFNEKTEKETD"
     gene            complement(7101..7616)
                     /locus_tag="PK252_08115"
     CDS             complement(7101..7616)
                     /locus_tag="PK252_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14713.1"
                     /translation="MKKILIILLVVIVLLMIISAVKIELYTSNYSNNIQTNWNNSKGI
                     VWLSGKVGKMEDLPQDSFPVVVKYDTLVVGDIHEPHPIVVDLNDVDFGPIWMPFYKAS
                     DYSISVNLKKEYEVNTDKANIKYRIDGQISISGKYKIVGLCSTQKAKELVIRDAMEKI
                     YSDAKINGIKL"
     gene            complement(7780..9765)
                     /locus_tag="PK252_08120"
     CDS             complement(7780..9765)
                     /locus_tag="PK252_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT14714.1"
                     /translation="MNKRFNLFFAVAFVISGQLCLFGQSSFETYTNPVIPGDHSDCTL
                     TKVGNDFYTTGSSFNPTPVIYHSTDLVHWEAIAQPVAADWSEYGDAPQGGCWGGQIVF
                     YKNKYWDFFSRANTMYYVIADKPEGPWSMPVRIKDPSQLSYGLGYDNSIFIDDNGKWY
                     LVIKNGQPNGGIVELGDDGQPTGIVYDLNWLNAKISSNPDVYQYSWAEGPVMWKHNGF
                     YYYSFARDVSGGQKVMRSKTLTADKSAWTTPVDFFNENDPDKANAIFQGPNHSSAAVE
                     LADGTSWVLHPVWARNSGEWFGQGRQGILNQVRYIGDEVVADYPANKSFTAPKLPSSG
                     IPWCVAKSDCFVSDKLNPEWSFLGFTPKNTWSLTERPGWLRLYPKSPTKANTVIKTDA
                     EHNYSLITRLDFDAKSTNDEAGLRLMNGLENLYIKLYSSVNSKGQKVFAIKFDSVYAE
                     VENKIGNVAWLKLVRVNHIMTGYYSADGSNWQQVGHEINIAALDKFNKDWNGWCGNRQ
                     GLYVAGKSADFDLYIYRDAYTPILAECPANQFGTTRTQTASKDYSLDSIHNNDWALYA
                     GVEFGNDDYKKKPYIVKMTAACTTKGSAVEVWIDAIGTGQKIATCNIESTGGVDAYKT
                     FLAKTATISGKHDVYLRFVGSEKARLFNLKSFCFEAK"
     gene            complement(10028..10183)
                     /locus_tag="PK252_08125"
     CDS             complement(10028..10183)
                     /locus_tag="PK252_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14715.1"
                     /translation="MAVQQMITLYILALMYDKNKLIQQHSCGIFVRKEIYKLIEATQF
                     LTIYQSI"
     gene            complement(10312..11355)
                     /locus_tag="PK252_08130"
     CDS             complement(10312..11355)
                     /locus_tag="PK252_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5627 domain-containing protein"
                     /protein_id="HOT14716.1"
                     /translation="MMKKLIFISLIAISFFTSCKNEDNEFADYKYTTVYFPYQYPVRT
                     IVLGKDLYDNSLDLAHKCKIMATMGGVYSNNSDRILDVEVDNSMFDTLLFDGEAGKYI
                     LPMPSNYYTLPSNMQIVIPAGSISGGIEVQLTDAFFADKKSTQATYVIPLRIKSVKNA
                     DSILSGKAAVTNPNLFVASDWSIAPKNYILYCIKYINPWHGAYLRRGVDVGKGSAGYS
                     KYDTVLTYHKQYVERDTIAKMYTVSMNECLMTVKTRNARSKKDVTFSLIIKVDDAGKC
                     VVSAPDTVSYKISGTGEFVKDGDMWGGVKQDVMRLKYSVDFDTITHTLTDTLVMRDRQ
                     VVMQTFTPAILKK"
     gene            complement(11459..13216)
                     /locus_tag="PK252_08135"
     CDS             complement(11459..13216)
                     /locus_tag="PK252_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14717.1"
                     /translation="MNTKLCILLALLLVFAGCEDVLSPADENNRELESIYEDAAFAEG
                     LLLNGYLRLPQGSYSYSDVATDDAVTNNVNSSYLLIATGRWSSMFNPTDEWTRCYAAI
                     QYLNIILAETDSVKWALTGKYVKEMFNDRTKGEAYGLRAYFMYYLLRAHGGYTADGQL
                     MGVPIYTKPVYTTEDFTKPRSTFKECLEQIYSDLKLAESYLPLDFEDIKDSLSIPAKY
                     LGKTTVSDYNRVFGAYNHQRLTARIVKGIRAKVSLLAASPAFSQGTSVTWEQAANDAA
                     EVLNLNGGLAGIAKNGWYWFSKANASEIGALAKGVNPPEVLWRTNVVDDNALEIDNYP
                     PSLYGKGYVNPTQNLVDAFPMANGYPISDPNSGYDANNPYANRDPRLAQYIVVNGSKV
                     GPKNTKIFTKVGQTKDGLNSISTSTRTGYYMRKLLREDVNLDPVSITKQKHYVPRIRY
                     TELYLAYAEAANEAWGPDGTGTNGYSARDVIAAIRKRAGIEQPDAYLASINNKEDMRK
                     LIRNERRLELCFEDFRFWDLRRWKESITEPAKGMMITADDQYVINTVENRQYSDYMYY
                     GPIPNSEVLKWGFVQNAGW"
     gene            complement(13338..16151)
                     /locus_tag="PK252_08140"
     CDS             complement(13338..16151)
                     /locus_tag="PK252_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT14718.1"
                     /translation="MKHIRIRMVLFGTCLGFTLAVSGQTETVEVAKVDSSKTDSIVNV
                     AYGTVHRNDLSNAISVLNPTEYLDKNYGTYPGDGIAAFVGGSNFWNVGNTLVLVDGVP
                     RPINEITTTEIQQITFLKGANAVVLYGSRAAGGVLLITTKRGKAGLRKSNVYVNTGIN
                     IPKSYPDYLNSAEYMKYYNQACINDGLVPLYSDETINNYASHSNIYRYPDVDYYSSDY
                     LRSMSNYNRADADFTGGNDRAQFYAQSGFQHSNSLLNFGEGKNENVTRFNIRGNIDLK
                     LNDYIKTYINMSNVFYDARSANGNFWSNSAKIQPHRYSPLIPVDMIAKDSTKAQLLAS
                     ESRHVIDGKYILGGTQEYLTNPIADVYAAGYTTYTSRQFQYNAGAIVDLNNAISGLTF
                     ATQIAVDYSNSYNEVVSNTYAVYLPTWNNSVANDNTIYGLTKYNKDKNAGEAKTLSDN
                     WNSQVLDFNMHFDYDKTFGDVHNFSGKLIAAAYRGRQTGDYQYRTNSNLGLQLAYNFD
                     HKYYLDFSSAVVNSTKLSTKKRVALSPTASVGWVLSNEEFLKGSDVVNRLKVSVSGGI
                     LNTDEGLSGYYLYNTSYVSNGWYSWSDGSWSNQATVVTRGANENLTYAKRKEITFGLE
                     GDLFNRLINFQTNVFYIKKDGIPVQNSSQYPSFFVTGWPQTSFLPYNNYEENSYKGLD
                     FQISFNKKLGDVNLTVGASGTYVVTEALVRDELYQDKYRNRAGKPINAIFGLQNEGFF
                     MDEKDIETHAKQKFGEVKPGDIKYKDQNGDNIIDENDEVMIGEWGNPFNFGVHITAQY
                     KAFTLFVLGTGGLGGTATKSGSYYWVYGDGKYSAVVRDSWTEATKNTATYPRLTTLSG
                     SNNFRASDFWTYSTNRFDLSKVQLTYTVPERFFKDSYIKGLNLYVNAFSLLTISDNKD
                     IMELNVGTEPQTRFYNIGVKAVF"
     gene            complement(16184..18022)
                     /locus_tag="PK252_08145"
     CDS             complement(16184..18022)
                     /locus_tag="PK252_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT14719.1"
                     /translation="MKKTIYKIFGAIVLFSFIGLFSCTEYLDKAPESEISSTDAFINF
                     ENFQGYTEEIYACIPDYIGTVWACDWLLADEVLHKEGGIWLNDLFDKGDYWGWTNSAW
                     ISYLDGNMSNGNNGVNTSTTSSFDKDLWPNAWYAIRKANLGLANLDNLKNATQEEKNI
                     IKGQLLFFRGWFHFELMSYWGGLPYINKVLSSSEKLTLPRLPYQQIADSVARDFREAA
                     DLLPVDWDKTEAGSRTLGKNQQRITKVTALAYLGKNYLYAGSPLMNKVSGGDAAYNTE
                     YCKKAAAAFAEMLKIVDSGETWVKLVSFADYSSLFYTDGRSTIPGYPEAIFQAPVYSS
                     WFKGCPWGPSAIFCDANIGGGYVSPNARYVENYGMANGLPIDDPESGYNPADPWTGRD
                     PRFYNDIVTDGQQVIKGSASADKEKYRYASLYNGGASRNNQGSGRTGYLIRKLTPMSA
                     NDIDGYSNNYMHLSYMRLADVYLMYAEAVLQGYGSATSSYPGYITAEEAFNKVRTRCG
                     AGAVAAKYVSDKDKFMGEIIRERAVELSFEGDFRFNDLRRWMIAGEKKYREKTALDFD
                     RGADGKPVNIKERVILTRPFESKHYWLPLKISDVTLYPEFGQNPGW"
     gene            complement(18033..21050)
                     /locus_tag="PK252_08150"
     CDS             complement(18033..21050)
                     /locus_tag="PK252_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14720.1"
                     /translation="MKGVIVDPDNLPVVGATVVVKGTTTGVVSNQNGEFSINVPTSGE
                     QILQISFIGLTPQEVDVKDKTTVNVAMTVSVTNLEDVVVVGYGMQKKESVVGAITQTK
                     GETLQKTGGVSSVGAALTGNLPGVVTVATKGTPGDEDPKIYIRGQSSWNNSDPLVMVD
                     GIERKMSSVDISSIESISVLKDASATAVYGVKGANGVILITTKRGHEGKAEIHVSANA
                     TMKVPSKLATKADSYDALSVRNEAIIREVSMNEASWEEYTPVAILEKYRNPANQEEAE
                     RYPNVDWNDELIKDYAMSYSANINASGGTSHVKYFTSVDFLNEADILKKIDNGKSYDP
                     GYGYKRTNVRGNLDFYLTKTTTLSSNLAGSYGIKQDCYNQDAWEYRIWQSIYSNPPDA
                     YLPRYSDGAWGYMEDYEVSTVNSLATMGNNGIRKTSTIQLNTDFTLKQDLSMLVNGLS
                     VKGSISFDNIFKTVGGIYDNGNVQQKYINPVTGEVTYSRYLGTNQFDWVPTQWSINTD
                     APYNNGSGAGSDNYRKLFYQGQIDYAKKIDEHDFTAMGLFSRDRNATGSEFAHFREDW
                     VFRTTYNYASKYFIEFNGAYNGSAKFGPENRFAFFPSGAVGWMISEESFMKNLTFLKS
                     LKLRASWGQVGDDNISTSRWLYMTTWTNGGNSPLGSQAGNTSPYTWWTESKIGNPDIH
                     WETTTKTNFGVDFSFLKDLISGSVDVFQDSRKDVLLAGGSRAVPSYFGGTPPTANLGK
                     VRVRGYEFELKFNKIFANELRVWANFSMAHSKDEVLEADDPILKDDYLKAAGKQIGQT
                     RTHVNGTFYNTWDELYSSTKLNTYDAQKLPGNLYMIDFNGDGVVDGYDRVPYGYPERP
                     QNTYNATIGAEWKGFSVYFQFYGVNNANRYVSLSNFSEHLDRVYNIGSYWTPQNTDAS
                     FPMSRWNTHNDFTGTTYLYDGSYLRLKNVEIAYTFGKDALKKIGISSLKIFLNGNNLY
                     MWTHMPDDREVNMGASSAYPTVRRFNLGLNITL"
     gene            complement(21667..22227)
                     /gene="ruvC"
                     /locus_tag="PK252_08155"
     CDS             complement(21667..22227)
                     /gene="ruvC"
                     /locus_tag="PK252_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT14721.1"
                     /translation="MVKKRVILGVDPGTNITGFGIITSDGKSPVLLIIDVINLSKEKD
                     IYRKLQIIFDKTLELIDHYQVTELAIEAPFFGKNVQSMLKLGRAQGTAIAAALFRRLP
                     ITEYAPRKIKLAITGNGDASKEQVASMLQKMLNLKTLPQDLDATDGLAAAICHYYQNG
                     MIEGAVGSKAKSWKQFLADNPERIKK"
     gene            complement(22273..23244)
                     /locus_tag="PK252_08160"
     CDS             complement(22273..23244)
                     /locus_tag="PK252_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT14722.1"
                     /translation="MGTINWGIIGLGRIAEKFAHDLQFVCNAKLYGVASREKDKAEEF
                     RVKYKAEKAYGSYEEMLQDANVDVVYIATPHVMHAENTIASLNAGKAVLCEKPFAMNG
                     QEVQAMVDKAREKDIFLMEAIWTRFLPTFLKVEHLIAERVIGDILQIKSDFGYQAVYD
                     KEWRLFNKKLGGGSLLDIGIYPVFLTLQLLGKPENIVTSAVIGETGVDESWVAGFTYK
                     NGATASLCSTFMANTPVETDICGTKGRIKMHRMWHCPSYLTLTMNDGAETVFKFDVKG
                     FGYEYEAQEVTNCLLTGRKESALLPLSFSISLMEMLDDVRERIGLHY"
     gene            complement(23277..24530)
                     /locus_tag="PK252_08165"
     CDS             complement(23277..24530)
                     /locus_tag="PK252_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT14723.1"
                     /translation="MIAYAAVDDTGYFRVEINDCEPKEIYSYLGIYKCYIYLVPGKHY
                     RIILPKKIEKTMQDKLNPFFEPVELPFGIYPNHEGVNRQIAEFDFKYAPFFRSLSANV
                     RPVRKNPLLDSALNVLSAMVDTIKDEYVKEYCNYRIGYLMAIGMVQKSRVVAGTYFEN
                     KPVLYNNTAYWQLFNFLYAKFFVYMGRGKVTKKIFEDINTYKSFDSLQLTLAGINDLK
                     SDTLRELVILKNLHDEFYSTNFSRSGILAVLDTLITRTKLPLHKQYALQIRSKITRLM
                     PDYAPPSFALYDIAGKRHELADFKGKYVYLNFCSCNSYACFKEYDLLKKLSERFAGKI
                     TIISVIADGSVEDLANFVKKSDYRWLFLHYGNQPEVIKQYDIRGFPVYYLIGPDGKLI
                     LSPAKGPTENIELELFKIMRSRGDL"
     gene            complement(24672..26078)
                     /locus_tag="PK252_08170"
     CDS             complement(24672..26078)
                     /locus_tag="PK252_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14724.1"
                     /translation="MNNISKILFAVILFVVPAFLSAQELDWEKMLIQDVENINPVYKP
                     VIGFGTGYINYMGDIRNNGSSIFAGSLAFKVNMHAYIDAQRHYKFNFYTLFTVPGNSK
                     TSMTVIQRDYTDPSKNFRFTSDLLTFGLNAHYDFDHFISQKSLFRPFVSLGAELVMFS
                     SKANLMNGNNMFYYWSDGTIRDKIQSAEQISTIVKPDDEYETDMRSNTEINKGEKYNQ
                     YSIAVPLEIGVQFDVTNRTSIRLGYAFHLNFTDNMDNVSSSNPIQSTLYNLNIKDNGS
                     AFDNFGYSYVSLHFDLFSDPKMLRNKLLFLDLEGYDYDLIGDEDGDNVFDINDKCLHT
                     PKGLAVDTAGCPEDTDKDGVPDFMDKQNDSPVGAIVDKDGVEIPDNLMWDNLGLEALP
                     RDQVEALLAITNNMSAGSGRRVGAMDIPEKFKSLDVDGDGYISFDEVLKAIDSFFDFD
                     SELNTSDIYELNDFFFAQ"
     gene            complement(26081..27466)
                     /locus_tag="PK252_08175"
     CDS             complement(26081..27466)
                     /locus_tag="PK252_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14725.1"
                     /translation="MLKWLIIPVNVLISLFAGLMFDGDVNVRVTAPQSVTAGDEFQVK
                     VVVQKGKVQSFSRFLQELPMGLKATAAYSANADFTFKDNKVRLIWLKMPETDSVEITY
                     TIKVDPRLKGSFDLGGKFSYIDNNERMSVESELLPITINPNPNIDPSLIVDIMDFKEK
                     VIPELPTGKEQNVACFREKPVPANDADNSYIVNLLVYKENAQKFAKIEEIVPQGYTAL
                     RIDSKEGMFIYKDGRAKIMWMNLPSNNYFIVSYRLVPNKAGIEAPVLKGQFSYMSDDR
                     TIVKDIIEKDVMLASLTEEEIKKLIEESKVGNVQVPISELIAVQPKVEPKDCAMVARQ
                     EPVKKVIEPLEKKPVQKKTGSSINASDQLEAQEGIYYRVQVAAGHKPINIKAYFKRYK
                     IADEVRAEEHEGWRKYSIGSFAEYKQARDYRVHIWNTTPLTDAFVSAYNSGKRITVQE
                     ALMITNQSWYR"
     gene            complement(27665..>28272)
                     /locus_tag="PK252_08180"
     CDS             complement(27665..>28272)
                     /locus_tag="PK252_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOT14726.1"
                     /translation="VEGFEKLARIGPCVSIFGSARTKPDNKYFRLAEEIAYKLTQYGY
                     GIITGGGPGIMEAGNMGAKKGGGSSVGINIALPFEQEPNLFIDNDKLITFDHFFVRKV
                     MFTKYAQGFVVLPGGFGTLDELFEAMTLIQTGKIGRFPIVLVGKDYWDGLVAWIREVL
                     LEDESNVSPADMELFTIVDTADEAVEIINSFYAKYLLSPNF"
     assembly_gap    28273..28372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28373..29187)
                     /gene="xseA"
                     /locus_tag="PK252_08185"
     CDS             complement(<28373..29187)
                     /gene="xseA"
                     /locus_tag="PK252_08185"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT14727.1"
                     /translation="MENKIFSLYEINSLIAGVISDNLSNNYWIVSEIARISENSSGHC
                     YLELVEKDAVSDTVLAQAKAMVWSRNYKMLKAYFETATGRRLERGMKILFKASVQFHP
                     LYGLSFHIVDIDPGYTIGDLALKKMQVVKKLKEDGVFELNKIVQMPLVVQRIAVVSSE
                     GAAGYSDFMAHLAENPHGYRFEVTLFQALMQGEKAEASIIAALDAIFAKIEKYDVVAI
                     IRGGGGQVDLSCFDSYLLASHIAQFPIPVLSGIGHERDDSVVDMVAHTRLKTPT"
     gene            complement(29188..>29487)
                     /locus_tag="PK252_08190"
     CDS             complement(29188..>29487)
                     /locus_tag="PK252_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14728.1"
                     /translation="PDFYKAYQILARENNANHVGFLAYPNPFSTLLNINTYFENSGIT
                     TLKCFNLYGQQMFSLQNEKDFIHLQGELQYLREGMYIFTIERNGQRRNFVGVKKR"
     assembly_gap    29488..29587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29588..30689)
                     /locus_tag="PK252_08195"
     CDS             complement(<29588..30689)
                     /locus_tag="PK252_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT14729.1"
                     /translation="MKIIWCHDSIVEYTIYIILFFFVATNSLRAQDVELYRVTFKDKA
                     GQAYLLDSPNLFLSNKALQRRAKNNVKVDETDLPIKPAYIDSLTSKGIKIVNKSKWFN
                     CVTVDLGKNNISEVEKISCVLEARIIRKKSTFTSKRYWKKLEDAADYTEISFAQVALV
                     NGFSLHKLGYKGKGISIAVMDAGFGNADVYESLTNVRNDGRIADVADFVYPRKNIYKC
                     NTHGAMVLSIMAGEKDGILYGTAPEATYYLFRTEDAESEWPCEEDNWVAAAEYADSAG
                     VDVINTSLGYTTFDDTGFDYSYNDLNGKVSRISQGATMSASKGIIVVASAGNEGNNAW
                     KYIGVPADAEKVITVGAVDSTGVRAVFSSVGPT"
     gene            complement(30664..31470)
                     /locus_tag="PK252_08200"
     CDS             complement(30664..31470)
                     /locus_tag="PK252_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOT14730.1"
                     /translation="MSIKAYSTSVLIVYLLFFHLFAASCQSDTVWNTTDSKGMKQGHW
                     KSFYENGKFKYKGFFKDNKPVGDFVRYFEDGTIKAKQYFVPNTTISYATFYYENGQMA
                     AKGKYVGSLKDSVWQYFSYYDKTLRMKETYSLGKKNGVTMRIYNNGNVAERLVWKNNV
                     KDGEWLMYFENNLPKLEGFYKNDKREGMYKMYRPDGSLEMQGKFENNVMQGKWNYYDE
                     KGNVKMEILYINGVPQNASQLEENQRKIFEMIEKNKGLIPEPDENNMVPR"
     gene            complement(31547..32266)
                     /locus_tag="PK252_08205"
     CDS             complement(31547..32266)
                     /locus_tag="PK252_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT14731.1"
                     /translation="MKNDLEPENDVMSYLDGNSGYGQNLSCSIQPDALSKLGPFYYFC
                     YDYAKKKYSFISDSVYTVLGYNPYNGADCNMLGLAESSYHPDFLNTQKAIYNHIEKYV
                     KELSASQISKYIFRYHCWVQNKNKHYIKLNISLRFLKKKSNEILASMIGLCFKTGEGI
                     PKDNIQTLTVYKSINNTEKKVFEKDFIVEYENGILTKKETQVWLMLKDNMSGKEIADE
                     LGLSIHTVNTHRKNIYKKLKN"
     gene            complement(32499..34397)
                     /locus_tag="PK252_08210"
     CDS             complement(32499..34397)
                     /locus_tag="PK252_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT14732.1"
                     /translation="MGTIVQLFEDSVAKYSGNPFLWEKIKDQYQSLTYAEIHQQVIFF
                     AKGLLSLGLRRGERVAMLLEGRTEWVVSELGILYCAAVNVPLSVKLNEPDELLFRLKH
                     SGAKFVITSGYQAKKIVSLLPSLPLIEKIIVVDDEPLGFSLEISYKEVVCMGKNAGID
                     IQSELEKRIASILPDDSSTICYTSGTTADPKGIILSHGNYYCNTTQALGLIAVPSYFV
                     TLLILPWDHSFAHTAGIYTVAKAGASLAAVQSGKTPLETLKNIPVNIKEVRPHFLLSV
                     PALAKNFRKNIESAIQAKGVIVQWLFTTGLKTAYLYNGNGYNKGKGWRVLFYLPVRLF
                     DAILFKKIRENFGGRLQFFVGGGALLDIELQRFFYAIGIPMLQGYGLTEASPIISANS
                     LTKHKLGSSGSLVPNMDFTIRDENGQVLPQGEKGEIVIKGGNVMSGYWQNEEATRQTI
                     KDGWLYSGDMGYLDKDNFLYVLGRFKSLLIADDGEKYSPEGIEEAMVSQSSFIEQCML
                     YNNQKPYTVALIVPNIVAIKRKLSEHHHTPTPEAVLKLIESEVNEYRTGRKFGHMFPQ
                     RWLPAAIGVLNEPFTEENHMLNSTLKMVRGKVVERYEKLIIFLYTPEAKNVCNSLNMA
                     SAEKLLKN"
     gene            complement(34405..35346)
                     /locus_tag="PK252_08215"
     CDS             complement(34405..35346)
                     /locus_tag="PK252_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14733.1"
                     /translation="MKYSKLIVLFFSACLILVSCSDKGGGSSVGADENTITYKDADTS
                     LVYNIQDYKVIYGGDTTTLVFNALDDSLKTALKITFYKIVNLSNTSYEVITSINDTSG
                     VRATYRDESNVEHEFSAGTVEVAKYQKGSVLQARYKFVTYETDLINGIDTLECEGEIN
                     LNMVLFDPDRIPNIDIKPASMTFRVNDTLRSYNSVATHISGNGIDKYVINGTWQNQTL
                     VIEMSDIELKMNKEYPIGQLIESVGFIKANYSSDRNTTYWADGRKGTSGKIKIYKIVE
                     NNTLQGYFEFVGVIPADTSKKARITQGKFYTKLKRIE"
     gene            35580..36236
                     /locus_tag="PK252_08220"
     CDS             35580..36236
                     /locus_tag="PK252_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14734.1"
                     /translation="MNSKVKLLIVDDHDIFRQGVKMLLNNDSAVEVIGEASNGKEFLD
                     VVEKLAPDVVLMDIAMPVMDGIEASKLAHEKFPNLKILALTMFGDEKYYYPMIQAGIK
                     GFILKSSGISELQNAINMISQGFNYFSNELLCKLIKNVSEKNSVPDDKNDKLTKRELE
                     ILKEIACGLSNDDIAQKLHISLSTVKTHRNSLLQKTGTNNTASLIMHAIKNKLLVVEE
                     "
     gene            36437..>38229
                     /locus_tag="PK252_08225"
     CDS             36437..>38229
                     /locus_tag="PK252_08225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT14735.1"
                     /translation="MDPIIQKFREKFFDEAGRLLDQLEKDLLELEKDLSNKELKESAF
                     RAMHTIKGVSGMYGFDFICEFTHSIENLYQALRENKLNVERNFFDISFSAIDHIRKLL
                     VDEKLNDPTNQKNHHDLINQVNQIVQTAPPKPEPTIQKEEATTNENTEKASTWHILLR
                     TDEKMYFRGISLLNIFRELATIGEFQISRLDFMSQTESDTWSIILTSKASQDEITDIF
                     LFIEDNCSITQLSLEDVNGIFAHQNTEAQEESTDHEISIIEYIENQNNQTNQPKKEIS
                     SEVKEQKAVKQKEAIERKRISVDSQKLDQLMYLVSELITVNSQLEQSTKEVTFNSIRG
                     FLEKVDNLSKQFRNNALEIRLVPLSDTILRFQRLIRDLSKQMHKKINLETSGIDTELD
                     KSTIDNLNDPLMHIIRNCIDHGIETPEQRMAKGKPETGTIAISAYHSGNYIYIKIADD
                     GSGIDAEKVRKKAIDKGIWKADYQPTRKEIFDLIFLPGFSTAQSLTEISGRGVGMDVV
                     RKKIQNLRGDVEVESEQGKGTAFILKLQQSVTIIDTLLFTVNESNFIIPISDIQICSQ
                     YSLNEINLRRHTATLPYNNRLIPFIDMRQWF"
BASE COUNT        11809 a         8339 c         6305 g        11576 t
ORIGIN      
        1 ttgttctttg ttcttgattc taattcacat tctgaatcac ttttacatac ccatttttat
       61 tcccaaccat ataaactcct tttggaagtc cttctatagc atgctttctt attattttcg
      121 accttaattt tactcctaaa accgaataaa catccaccat ttcaagctca gaattgtttt
      181 caggcatatc attgatgcct gttggatcaa actcttgtga atttgacagg aaaacagtat
      241 caattacaaa ggcattgctg ccattagacc aaaatccggc aatataaata tggcttggtt
      301 caagtgtact tccatcccct tttttgacat ttttgaggtt gactacaact tggcgactat
      361 tgccaaattt accggaggtt gaagcacccc agtaattatt cccgtcaaaa agcttgaatt
      421 caacatccgc tttgttggca ctgcccaatt ttgcaaccag gtatttataa ccagacaaat
      481 caaccccttt ggaatattgc cagccaccga agccccattg gcctgtttta agcgttttag
      541 tagtttcatc aaaactgcca ttgctgaata tattagggtt caacaactca tttgtaagcg
      601 gaaatgttgt tgcattgatt ttaaaatcaa cctgtttcat tgtcccttgt gaacctttaa
      661 aacttgcagt aataacagca gcgccatctc gtacaacttc aatatatccg tttgtcatct
      721 ttacaacatc aggattgctg acagaataac ctgctttatt ggcaacatca agggtatgtc
      781 cgtctgcata aatggcagtt aaagacaacc tctgtattga ccctgtcaac aaagtgtagc
      841 tgtttttttc aacactcaat gaatcaaccg tatttccttc aaccgtgtta taggttttta
      901 acagcgagag catctgcaac ccgtaatgtc tgcccaactc acgagcgcct tcagtagaaa
      961 aatgcaaacc atccttgcca ttgcctgcac atcctttaga agatattgca taagcattag
     1021 ggatataata aggcaaagta gcaataatgc cgttcatgcc ccagcaaata cccccttcat
     1081 ctttacccag caattcacca gcaagcaaag gaattgagtt tgcttcaata ttcaaatctg
     1141 ccagcatgtt gttataaatt ttcttaacct tttcgggcca atcggtttgc ccattattgc
     1201 tttcgccctg atgaagcaat atgcctttta tcaccccttt tttctgggcc attttggcca
     1261 tttctataat cttggcataa gggttgccgc catagatatc cataagccct ttctgccatg
     1321 catcggcatt ctggtaatac tgcgtataat tgtccttatc aaaagcatca ataccgctgc
     1381 cacccatggc aacaactatt acaccaactt ttatgctgtc aggaaggcta tcaacgagaa
     1441 cccggccaaa ataatcagcc ggggtaagcc cgttgtccca tcggcataat ggaggcttag
     1501 cggtgtacca atttcctgcc gaccttccat tatgcagaaa atcaccagca ctaactgtca
     1561 tcactttgaa acgttcgcta acacctatag tatcagcggc agcaatagct gcattacctt
     1621 ccatattgga ctgcccaaaa catatataga tatgaaaatt gggatcaggc gaatccaatg
     1681 cttgcgcata attgccaaac aatataaaac ctgtaataat tgcaataaaa tacttcataa
     1741 aacctttctt atttataaaa aaacattacc aaccccacac tattcgttta ctaccctctg
     1801 ttttcttcca atagcaatat ataatatgaa gcccaaaaaa tttaagcaca gtactattat
     1861 gacccaaatc aacttgttat tgccttcaaa tctggatttc agaatatcta ttaaggcaag
     1921 tactggcaac agcattgcca atacaaccat aataagaccc aatataacac ccgttcctgt
     1981 gattaattcc atacaatcaa ttgttatttc tcagcctttt cgtctcctaa tttcaaaaca
     2041 taactaaaat ctgtatcatc agactttacc tggtattttt ccaacggacg ggggccacag
     2101 ctggccgaac caacacccag tgtctttgct gacaagcaga atacagttgc tgtacttgcc
     2161 ggaaggtcaa tcttatattc cacagggttc atctgctcat cggtataagg caatgccgag
     2221 aactgcatca gtttttcatc ggcttttacc tcaaacaaag gcatgtcggc accgttgaat
     2281 ttcacccaac gcatttcctc acggttgcca tactccatag gcttttcata ctcgtattgg
     2341 ttgtttacct ccaattcgta taagccaaca tcctgggcac ttttgcggtc ggcatagttt
     2401 tcgaccgggc cacggccaaa ataattgagg cggtcgtact ttttgtcgag caacatgcgt
     2461 accccaatac gggcaaggtt gatacgaaag ccagtaaact gcactttatt gtcaaccttt
     2521 acagtgccgt caccttttat agtatataca gcagtatgat atgctgcaaa cccttctttc
     2581 ccttcagctt taatggtggc agtaactttt gcagcagtat tatcaataat ttcagttttc
     2641 atatcaacca cagaatactt gaggtctttc accccgtact ttgaccattc tctatcgacc
     2701 caccaatcgt cggtttggtg cattgcccta tataaatgca atttgggggc accgtcagaa
     2761 gtcaggatat tcttgccatt cttttcaatt tgggtcaaaa agcctgtttt tttatcaaag
     2821 gtgagagcaa aacccgaacc tgaaaccttt acctgctggg catcctgaat aagcttaaca
     2881 ggttgagtaa ctttagcttc aaccacaggc aaagtcttta tgggcagctt aaactgttcc
     2941 caggccactt catagccttt gtcagcccat aatgttttag ttttttgtgt aaacgaaacc
     3001 cgcacaaaat attcagcccc ggcttttggg ttttctattt tataagggat gatagcgata
     3061 gtgcgccccc aatggttgcg gatttctaat gagcctttgt ctatttcttt tccattttcg
     3121 gttaagctcc atgaggcatc aaagccatct aaagagatga actggtattt atttttaatt
     3181 tgaatttcgc cattagcagg gtcaacaagg cttatgtcta tccattgaaa agcctttttc
     3241 atttcaggga aatgaggttt aacacgggtg cccccggtag cacggtcaaa ggaaacaacc
     3301 cctttatgaa taaaataatg gtcgttagga gcttcgccaa agcctccgcc ataagccaga
     3361 atcggatggt tagggtcgcg cttgttccac agcgcctggt cttgaaactc ccagatagcg
     3421 ccgccaagta tctcaggatt attgtcaaaa gcttcagaat agtcggtcaa cgagcccata
     3481 gagttgaaca tggcatgaac aaactcgcag atataaagcg gctttgtcaa accccagtat
     3541 ttaaccatgt ttacataatc ttcggcagtg ccatacatgc ggccatcgag gtcagcaggg
     3601 ttgcggctgc cagcgccaaa gcgctcgtaa tgcacaggac gggatgagtc tattgctttt
     3661 attgcattca tagcagcaac aaagtttgaa cccactccac cacactcgtt gcccaacgac
     3721 caaatgatga ccgaaggatg gttcttgaaa ttttcgacat tggccacgtt acggtcaatg
     3781 atggcggctt tcatggtagg ctcttcatcg aaacggccat cgtagccgtg actctcgcag
     3841 ttggcttcgg ctacaagcca aatgccccat tcgtcgcaaa gctcgtacca tcgtgggttg
     3901 tcgcaataat ggctggtacg cacatggttg caattgcctt gtttgatcac ctcgaggtcg
     3961 cggatcattt gttcttcggt aatggcatgg ccaacatctg accaatgttc gtggcggtta
     4021 acgcctttca gtttgatagg aacaccgttt acagtaaata cgcgcccttt gatttctatt
     4081 tttcggaaac ccacttttgt agaaagtatt tccccttcgg tacttgccaa tacaagggta
     4141 tataaattag gcgtttctgc agtccatttg gccggattgc taaccggaaa cttaatctgc
     4201 acttgctgtt cctgttttga agcaatagca gcggcagtcg cattgccttt tactacctct
     4261 ctgccctctt tgtcatatag tgtagctgtc agtgtttgag ccttggctcc tttgtcgcca
     4321 tagtttttga ttttagccac cacctcaaca gaagcatctt tatagtcttt gtctaagtct
     4381 tgttttacaa agaaatcgcg aatatgtact tgtggagcgc tccaaagata gacatttcgg
     4441 aatatgccgt gcaaacgcca catatcctgg tcttcgagcc atgtaccaga gctgtattga
     4501 tacacctcaa cagcaatagt attggagccg ggtttcaggt atttggtcag gtcaaactcg
     4561 gcagcattgc ggctatttac gctatatccg actttttcgc cgttaatcca tagaaagaac
     4621 gcacaatcta cgccttcaaa ggtgataaaa atgcgtcggc cggcccattc ggcaggaact
     4681 tcaaaatccc ttctgtagct acccaccggg ttgcgctctt tgtaagcggt aaaccaactt
     4741 tcgggagtac tcattatacg cggaaaatcc ttttttatgg tataaccaaa attgcggtaa
     4801 aaaggagtac cataaccatg tatttcccaa ttggaaggca caggtatttc ggcccatgat
     4861 gaaacatcaa aaccgggctt gtaaaagtca accggccgtt gttcagggct gggcacccag
     4921 ttgaacttcc agtttccatt gaggcttttg cacaaaccag atgccaggcg tttagcctgc
     4981 aatgcctcct gcaaatttgc ataaggcata agggttgcat gatatggttc tttgtttatc
     5041 ccaagcagtt cggggttttc tatttcggca ggataatcaa caccgggctt taaatcagcc
     5101 tgggccaata tttctgagga tttcatcaga aagccaacag taaaagctaa gagtaaatat
     5161 tttctcatca tgtctattaa tttatttctc aaataaaaac tatacagcat cacttattgc
     5221 taaattgcca ccaatcaaaa ttgaacaaat ctttctcacc tttgaacaca atgtataaat
     5281 cgtgaatgcc ttttacatct ttaacagaag ctttggcagt attccataaa tcaccttcac
     5341 ctgatgaaac aatattaata tctgccacaa caggcccgtc aatcttgtct atatgaaact
     5401 caatccttcc tccatacaaa gaggctacac ttacctcaac agtcttggca ccttcagcaa
     5461 aatcaacaga ctgaaccttg atataatctc cattgtggat ggaagtaaca tacaacctat
     5521 cggctatttt ctttccttta tcccacggga cattgcgttc ccattcagta gcaaattctg
     5581 ttttcaggcc ttcactaaaa gacattgttt ctgcttcatt tttcacaaaa gggtttaaag
     5641 tgccaacatt ggcaacgcct tttgctgacc accagggatg tttctgaatg gtaccatcag
     5701 cgttgtattt aatctcctca acacaaatag agcgcctttc ataatgttta gacatggtac
     5761 gcttcaacac attgtaattg aacccaaaga cataagtttt ccctttatat tctatgaccc
     5821 ccggatggtt tccggaagat ctcacgtctg gatccattat tgtaccttta catttccaag
     5881 ggccaaccgg gctatcgctc atggcataac caataccttc aggacaacat cttgaagccc
     5941 aggccagata ataatactta ttctttttcc aaagccaggg gccttctgta taggaagctt
     6001 cagcaggagt ccctgtaaaa tcaatagagg caacctgaat ctcacctttt agcgaaacca
     6061 tatcttcatt gagctttgca tagtaacaag ggccatttcc accccagtat agataagcct
     6121 gaccatcctc atctataaag acagttgggt cataatcgcc agggttagac ctaaaaatta
     6181 gcggcttgcc aagtacatca ataaagggac cataaggact gccggataca gcaacaccaa
     6241 tcgccatttt cccctggtaa atagtaggac aataaagata aaacttgccc tttcgctcaa
     6301 tgcattgagg agcccaggca ctatgtccat cagcccatgc aaaagtccga tatggctctt
     6361 ttacgccagc aataatgcca cggtcagtcc aattgaccat atctgtagag gtatataaca
     6421 tccaattttc catctgaaag ccaaaagcac cgtcttcatc atgactggta tataaaaaaa
     6481 cggtatcatg ataaaccaat ggagccgggt cggctgtata tttagtctga attattgggt
     6541 tatcagcaaa acaataagaa gcaaagcttg aaaatgctat tgctaatgaa cccagtttct
     6601 taaaaagatt tgtcatgatt agtaattttt aaaatatctt taataactta ttgaataggg
     6661 cgttatgccg aaaacgaaaa gccacagcta cacctaatcc gtttcctttt cagttttttc
     6721 attgaaatac accctgagtg cttttgaaat gtacaatacc aggatgccac agaacaacaa
     6781 cacgctaagc cctgaaaaat ctatgcgtat tgctctctca accagctgca acaacagtat
     6841 aaataagcaa ctccagagca tcccaattcc agaatatttg aaaaaatgga ttaaaaatgg
     6901 gatcatatca aaatgggccg aattggattg ggcttctatg cccgacttct tgcgaagaaa
     6961 atccatcacc gctgtaaaag cccataaaaa cacaaatatc aacaagatat aaataaacca
     7021 tttcggcagt tcgaggttga taaatgaatc gaagcttaat ctattgtcca tgttagtttg
     7081 tttattagtt tataaaagtt ttagagtttt attccattaa tttttgcatc agaatatatt
     7141 ttctccatgg catcgcgtat cacaagttcc tttgcttttt gagtagaaca caacccgaca
     7201 attttatact tgccagaaat cgagatttgt ccgtcaatgc gatacttaat attagcttta
     7261 tcagtgttta cttcgtattc tttttttaga ttgactgaaa tggaataatc agaagcctta
     7321 tagaatggca tccagatagg cccaaaatcg acgtcgttaa ggtcaaccac tattgggtga
     7381 ggctcatgga tatcaccaac aacaagagta tcgtatttaa caacaacagg aaaagagtct
     7441 tgaggcaaat cttccatttt tccaactttg ccacttaacc aaacgattcc tttcgaattg
     7501 ttccagttgg tctgtatatt attggaataa ttacttgtgt ataactcaat ttttacagca
     7561 ctaatgatca tgagtaaaac aatgacaaca agcagtatga ttaatatttt tttcatcggt
     7621 tggggttttt attctttata atacagttac aaatcaaatt actttattga attaggctct
     7681 taactaatat agtatctcat ttatcgcaga ttagcagcaa actgtgttta atctctgcta
     7741 ccttttgata tatttgctaa aagcctaatt caaatgtctt tatttagctt caaaacagaa
     7801 actcttcaaa ttgaacaatc tggccttttc cgaaccaaca aagcgaagat aaacatcatg
     7861 cttaccacta atagtagcag ttttggctaa aaatgtttta taggcatcaa caccaccagt
     7921 gctttcgata ttgcaagtgg caattttttg accagttcca atagcatcaa tccacacctc
     7981 aacagctgag ccttttgttg tgcaagcagc agtcatttta actatatacg gttttttctt
     8041 gtaatcgtca ttgccaaact caactcctgc atataatgcc cagtcattat tgtggatact
     8101 gtcaagacta taatcttttg atgctgtttg cgtgcgggtt gtaccaaatt gatttgcggg
     8161 acactcggcc aaaataggag tataagcatc tctgtaaata taaaggtcaa aatctgcact
     8221 tttcccggca acatacagtc cctgacggtt accgcaccaa ccgttccaat ctttattgaa
     8281 cttatcaaga gctgcaatat ttatttcatg gccaacctgt tgccagttac ttccatcagc
     8341 gctataatat ccggtcatga tgtggttcac acgtacaagc ttcaaccaag ctacatttcc
     8401 aattttattt tcaacttcag catatactga gtcaaacttt atagcaaaaa ctttttgccc
     8461 tttgctgttt acactgctat aaagtttaat atataaattt tcgagaccgt tcattaaacg
     8521 caacccagcc tcatcgtttg ttgactttgc atcaaaatcc aggcgggtaa taagagaata
     8581 attatgttct gcgtctgttt ttataacagt attggctttt gtcggagatt ttggatacaa
     8641 ccttaaccag ccgggccttt cggtcaatga ccatgtattt tttggggtaa aacccaaaaa
     8701 tgaccattca gggttaagtt tatccgaaac aaagcagtcg gatttggcca cacaccaggg
     8761 aataccacta ctaggcaact ttggagctgt aaagctctta ttagccggat aatcagccac
     8821 tacttcatcg ccaatatacc ttacctggtt caatatgcct tgacggcctt gtccgaacca
     8881 ttcgccacta ttccttgccc aaacagggtg aagcacccaa cttgtaccat cggcaagctc
     8941 aacagctgca gagctatggt taggcccctg aaaaatagcg ttcgctttat ctggatcatt
     9001 ttcattaaaa aagtcaacag gagtagtcca agcagatttg tcggcggtaa gggttttgct
     9061 acgcattact ttttggccgc cagatacatc acgcgcaaaa gaatagtaat aaaaaccatt
     9121 atgcttccac attacagggc cttcagccca actatattgg taaacatcag gatttgaaga
     9181 tattttagca ttaagccagt ttaaatcata cacaatcccg gtaggctggc catcatcacc
     9241 cagttctaca atacctccgt taggttggcc attttttatc accaaatacc actttccatt
     9301 atcgtctata aaaatggagt tgtcataacc caagccgtat gaaagctggc ttggatcttt
     9361 gattctcact ggcatgctcc atgggccttc aggcttgtcg gcaattacat aatacatggt
     9421 attggcacga gaaaagaaat cccaatattt atttttgtaa aaaacaattt gaccacccca
     9481 acaacctccc tgaggggcat ctccatattc agaccaatca gcagcaacag gctgggctat
     9541 agcttcccag tgaactaaat ctgttgaatg ataaataaca ggtgttggat taaacgacga
     9601 tcctgtagta taaaaatcat taccaacttt tgtcagggta caatccgaat gatcgcccgg
     9661 aattacagga ttggtatagg tctcaaatga cgattgccca aacaagcata gctgtccgga
     9721 aataacaaaa gccacagcaa aaaacagatt aaacctttta ttcataatac ttatcatttt
     9781 cactaaataa aatatgtaaa tcattaacta ttaattcttt atctcgaaaa gttcaatata
     9841 tacaattagg taaaatacaa agttatgttt accctttgca gaggacttta aatttgacta
     9901 taaaatatcc ttcatataag actaatttta ccttactttc attttttaat cattaaatga
     9961 atcatttttt taaatcgggg aaaaatttat ttttaattta aaatagcttg tatttaggcc
    10021 tcagacatta aatactctgg taaatagtta aaaactgtgt agcttcaata agtttgtaga
    10081 tttcttttct tacaaatatg ccgcaactat gttgctgtat tagtttattt ttatcataca
    10141 tcaaagccaa aatatataag gtaatcattt gctgtactgc cattttgttg agttaaaaaa
    10201 aagggttgag gcacattttt gaccgcaacc ctttctaact acaactaact actaaacaag
    10261 atgtttagac catcttcttt ttaagaaatt atgattgaaa aattaacaac actatttttt
    10321 cagtatagca ggagtaaaag tttgcataac aacttgtctg tcgcgcataa ccaaagtatc
    10381 agtaagcgta tgggttatag tgtcgaagtc aacgctatat ttcaaacgca ttacatcttg
    10441 ttttacacct ccccacatat ctccgtcttt aacaaactca cctgttcctg aaattttgta
    10501 agaaacagta tcaggggccg aaactacgca tttaccagca tcatcaactt ttataataag
    10561 agaaaatgtt acatctttct tacttctggc attccgggtt ttaacagtca tcaaacactc
    10621 gttcatcgat acagtgtaca ttttggctat agtatctctt tcaacgtact gtttgtgata
    10681 agtcaaaaca gtatcatact ttgaataacc tgcacttcct ttgccaacat ctacacccct
    10741 gcgaagataa gcgccatgcc agggatttat atatttgata caatacaata tataattttt
    10801 gggggcaatg gaccagtcag aagcaacaaa caaattagga tttgtcacag cagcttttcc
    10861 acttaagatt gaatcagcgt ttttaaccga ttttattcgc aaaggaataa cataagtagc
    10921 ctgagtagat tttttatcgg caaagaaagc atctgtaagc tgcacttcaa tccctcctga
    10981 aatagaacct gcaggaataa caatttgcat gttacttggc aaagtgtagt aattagaagg
    11041 cataggaagt atatatttcc cggcttctcc atcaaacaat agtgtatcaa acatagaatt
    11101 atcaacctct acatctaata tcctgtctga attgtttgaa taaacgcctc ccattgtagc
    11161 cataattttg catttatgag caagatcgag tgaattgtca tacaaatctt tgcccaatac
    11221 aatggtcctt actggatatt ggtagggaaa ataaaccgtc gtgtacttat agtctgcaaa
    11281 ttcattatcc tcatttttac atgaagtaaa aaaggaaatg gcaataagtg atataaaaat
    11341 taactttttc atcatattca tttttttatt tttattgaat gatgaacacc cataaataac
    11401 ttttttatac ttgccacctt atggatgttc attttcttca agtgttatga tttaaatatt
    11461 accacccggc attttgaaca aagccccatt tcaatacctc actattagga attgggccat
    11521 aatacatata atcactatat tgcctgttct caacagtatt aataacatat tggtcgtcag
    11581 cagtgatcat cattcctttg gcaggttcag taatactttc tttccaacgg cgcaaatccc
    11641 agaaacggaa atcttcaaaa caaagctcca aacgtctttc gttgcgtata agttttctca
    11701 tgtcttcttt attgttaata gaagctaagt aagcatcagg ttgttcaatg cctgccctct
    11761 tacgaatagc agctatcaca tcgcgtgcag agtaaccatt agtcccggta ccatcaggcc
    11821 cccaagcttc atttgctgcc tcagcataag ccaaatacaa ttcggtatat ctgatacggg
    11881 gcacataatg tttttgtttt gtaatgctaa caggatcgag gtttacatct tccctaagca
    11941 acttacgcat ataataacct gtgcgggtag aagtagagat actgtttaac ccgtctttgg
    12001 tttgtccaac tttagtaaaa attttggtgt ttttggggcc aactttactg ccgttgacta
    12061 caatatattg ggccaaacgc gggtcacgat tagcgtaagg gttattagca tcatagccac
    12121 tattagggtc gctaatagga taaccattgg ccattgggaa tgcatctacc aagttttgtg
    12181 ttgggtttac atatcctttt ccataaagcg atggaggata attgtcaatt tctaatgcat
    12241 tgtcatcaac tacattggtt ctccataaaa cttcaggagg gtttacccct ttagccaatg
    12301 cacctatttc agaagcattt gctttagaaa accaatacca gccatttttt gcaataccag
    12361 caaggcctcc gtttaggttg agtacttctg cagcatcatt tgctgcttgt tcccaggtaa
    12421 cagatgttcc ctgactgaaa gcaggactgg cagcaagcaa gcttactttg gctcgtatcc
    12481 cttttacaat gcgggctgta agacgttgat ggttataagc cccaaaaacc ctgttatagt
    12541 ctgaaacagt tgttttgccc aaatattttg ctggaataga aagagaatct tttatatcct
    12601 caaaatcaag cggtaaataa ctttcagcca gctttaaatc actatagatt tgttccaaac
    12661 attctttgaa tgtactgcga ggcttggtaa aatcttcagt agtataaacc ggcttagtgt
    12721 aaataggtac ccccataagt tgcccgtcag cagtatatcc tccatgggcc ctgagcaaat
    12781 agtacataaa ataagccctt aagccataag cttctccttt tgtgcggtcg ttaaacattt
    12841 ctttaacgta tttgccagtg agagcccatt ttacgctatc agtttcagcc aggataatgt
    12901 tcaggtactg aatggctgca taacaccttg tccattcatc tgttgggttg aacatggacg
    12961 accaccttcc ggttgctatt aacaaataac tactattaac attatttgtc acagcatcat
    13021 cagtagctac atcactataa ctatagctgc cctggggcaa acgtaaatat ccgtttaata
    13081 aaagcccttc ggcaaaagca gcatcttcgt atatactttc aagctcgcgg ttgttttcgt
    13141 ctgcaggcga aagcacatct tcacagccgg caaaaactaa aagcaaagct aataatatgc
    13201 atagttttgt gttcatccga acataattaa atcttaaaag tcggatggaa cccgtcccga
    13261 ttaatcggga tttatacgag tttttattta tttctataca tgctcgtttc atattcattt
    13321 catttaaatg attttcatta aaatacagct ttgaccccta tgttgtaaaa ccttgtttga
    13381 ggctcagttc caacattcaa ttccataatg tctttattgt cagatatagt caacaggctg
    13441 aatgcattaa cataaagatt aagccctttg atgtacgaat ctttgaaaaa cctttcgggt
    13501 acagtatagg tcaattgaac ttttgaaagg tcgaaacgat ttgtactgta agtccaaaaa
    13561 tctgacgcac ggaaattgtt gctcccgctc agggtagtca aacgtggata agtagcagtg
    13621 ttctttgtgg cttctgtcca gctatcgcga acaacagcag aatacttgcc atcaccataa
    13681 acccaatagt atgaaccact ttttgtagct gtgccaccca aacccccggt acctaacaca
    13741 aacaaagtaa atgccttata ctgggctgtg atatgcacac caaaattaaa aggattgccc
    13801 cactctccaa tcattacttc atcgttttcg tcgataatat tatccccgtt ttggtctttg
    13861 tacttaatat ctccgggttt tacttcaccg aatttttgtt ttgcatgggt ctcaatatct
    13921 ttctcatcca tgaaaaaccc ttcgttttgc aaaccaaaaa tggcattgat tggtttcccg
    13981 gcacggttac ggtatttatc ctggtacaac tcgtcacgta ccaaagcctc agtaacaaca
    14041 taagttccgg aagcccctac tgtcaggtta acgtctccaa gttttttatt gaaactaatt
    14101 tggaagtcaa gtcctttgta gctgttttct tcatagttgt tgtatggcaa aaatgaagtt
    14161 tgtggccagc cggtaacaaa aaagcttgga tattggcttg agttctggac aggaatacca
    14221 tctttcttga tataaaatac gttggtctgg aaattaatca acctgttgaa caaatcacct
    14281 tctaacccaa aggtgatttc ttttcttttt gcataagtaa ggttttcatt ggcccctctt
    14341 gttacaacag tagcctggtt actccaactg ccatcgctcc atgagtacca accattagaa
    14401 acataactgg tattatacaa atagtagcca ctcaaacctt catctgtatt aagaatacct
    14461 cctgaaacag aaacctttag cctgttaact acatcagagc ctttgagaaa ctcttcattg
    14521 ctgagtaccc aaccaacact ggctgtaggc gaaagtgcca cacgtttttt agtggacaat
    14581 tttgttgaat tgacaacagc gctgctaaaa tctaaataat acttgtggtc aaagttgtaa
    14641 gccagttgta acccaaggtt tgaattggta cggtactgat aatcaccagt ctgacggcct
    14701 ctgtaagcag cagcaatcaa ttttccagaa aaattgtgta catcaccaaa ggttttatca
    14761 taatcgaaat gcatgttaaa atcaagtacc tggctgttcc agttgtcaga taaggtttta
    14821 gcttcaccgg cattcttatc tttattgtat tttgtcaaac cataaatagt attgtcattt
    14881 gccacagaat tattccaggt tggcaaataa actgcataag tattgctaac aacttcgtta
    14941 taactatttg aatagtcaac agctatctgg gttgcaaagg ttaacccgct tatagcattg
    15001 tttaggtcaa caatagcccc ggcattgtac tgaaactgac gactggtata agtagtatag
    15061 ccggcagcat acacatctgc aatcgggttg gtcaagtatt cctgtgtacc acctaagata
    15121 tacttaccat caataacatg gcggctttcg ctggccaaca attgagcttt agtcgaatct
    15181 ttagctatca tatcaacagg tatcaatgga gagtagcgat gaggctgtat ttttgcagaa
    15241 ttgctccaga agttgccatt agcagaacgg gcatcataaa atacattaga catgttaatg
    15301 tatgttttga tataatcgtt cagcttaagg tcaatattac cgcgaatatt gaaacgggta
    15361 acattttcat tcttaccttc tccaaaattc aacaacgaat tactgtgttg aaagccagat
    15421 tgtgcataaa actgggcacg gtcattaccg cctgtaaagt cagcatcggc cctgttgtag
    15481 ttagacatac tgcgcagata atctgacgaa tagtaatcaa catcaggata acggtaaata
    15541 ttactatggc ttgcataatt attgatagtt tcatcactgt acaaaggcac taacccatca
    15601 tttatacaag cctggttgta atatttcatg tattcggcag aatttaaata gtctggatac
    15661 gatttgggaa tgttaatacc cgtgttgaca taaacattgc tcttgcgaag ccctgctttg
    15721 ccacgttttg tagtaataag caatacccct ccggcagcac ggctaccata caaaaccaca
    15781 gcattagccc ctttaaggaa agtaatttgc tgtatttcag tggttgtaat ctcatttatc
    15841 gggcggggaa ctccatcaac caacactaaa gtgttgccca cattccagaa gttgctacca
    15901 cctacaaaag cagcaattcc atcacccgga taggtgccat aatttttatc aagatattca
    15961 gttgggttca atacagaaat agcatttgac aaatcatttc tgtgtacagt cccataagct
    16021 acattgacta tagaatcggt tttggatgaa tccacttttg ctacctccac tgtttcagtc
    16081 tggcctgata cagcaagtgt aaagcccaag catgttccga ataaaaccat tcgtattctt
    16141 atatgtttca tattaaatat tttaaagtta aaaaactaca ctcttaccat ccgggatttt
    16201 ggccaaactc aggataaaga gtaacatcac taatttttaa aggcaaccaa taatgtttgc
    16261 tttcaaatgg acgggtcaat attaccctct ccttgatatt gacaggcttt ccatctgcgc
    16321 cacggtcaaa atcaagggca gttttttcac gatatttctt ttcgccggca atcatccacc
    16381 tgcgaaggtc attaaacctg aaatctcctt caaaagaaag ctcaacggca cgctcgcgga
    16441 ttatttcgcc cataaattta tctttatcgc tgacatattt agcagcaaca gctcctgccc
    16501 cacagcgggt cctcactttg ttaaatgctt cttcggctgt aatataacca gggtagctgc
    16561 tggtagcaga accatatccc tgcaatacag cttcggcata catcaaataa acatcggcca
    16621 aacgcatata agacaaatgc atgtaattgt tcgaataacc atctatatca ttggcagaca
    16681 ttggggtaag cttgcggatt aaataacctg ttcggcctga accctggtta ttgcggcttg
    16741 cccctccgtt atacaacgat gcataacggt atttttcctt atctgccgaa gctgaacctt
    16801 ttataacctg ctgcccgtct gtaacaatat cattgtaaaa ccttggatcc cttcctgtcc
    16861 aaggatcagc agggttgtac cctgattcag gatcatcaat tggcaaccca ttggccatac
    16921 cataattctc tacatacctt gcattaggcg aaacatatcc tccgccaata ttagcatcgc
    16981 agaatatagc gcttggcccc caagggcacc ctttaaacca cgaactataa accggagcct
    17041 gaaatatggc ttcaggataa ccaggaatag tacttcttcc atcagtatag aaaagcgaac
    17101 tatagtcagc aaagcttacc aatttaaccc atgtttcacc actatcaaca atcttgagca
    17161 tctcagcaaa tgcagcagct gcttttttgc aatattccgt attgtaagct gcatctcctc
    17221 cggatacttt attcatcaaa gggcttcctg catataaata attcttgccc aaataagcca
    17281 atgccgtcac tttggtaata cgttgttggt ttttacctaa agtcctgctc ccggcttcgg
    17341 ttttgtccca atcaaccggc aacaaatcag cggcttctct aaagtccctg gcaacactat
    17401 cggctatttg ctgataaggc aacctgggaa gggtcaattt ttcacttgaa gataaaacct
    17461 tattaatata aggcaaaccg ccccaatacg acataagttc aaaatgaaac catccacgga
    17521 aaaacaacaa ctggccttta atgatatttt tctcttcctg agtggcattt tttaaattat
    17581 caagatttgc taatccaagg tttgctttac ggatagcata ccaagcattg ggccacaagt
    17641 ctttatcaaa acttgaggtg gtactggtat tgaccccatt attgccattg ctcatatttc
    17701 catctaaata tgaaatccag gcactgttag tccaacccca gtagtcgcct ttgtcaaaca
    17761 agtcattgag ccagataccg ccttctttat gaaggacttc atccgccaaa agccagtcac
    17821 aggcccaaac agtaccaata taatcaggga tacaggcata tatttcttca gtataaccct
    17881 gaaaattttc aaaatttata aatgcgtctg tagaagagat ttccgattct ggagccttat
    17941 caagatactc tgtacaagag aatagcccaa taaaagaaaa taagactatg gctccaaata
    18001 ttttataaat agtttttttc atactccctg gtttataaag ttatattaag acctaagtta
    18061 aaacgcctga cagtaggata agcagatgaa gcccccatat ttacctcacg gtcatccggc
    18121 atgtgtgtcc acatatataa gttgttcccg ttgaggaaaa ttttaagcga actgatccca
    18181 atttttttca aggcatcttt cccaaatgta taagctatct caacattttt cagacgtaaa
    18241 taagatccat cgtacaaata cgttgtcccg gtaaaatcat tatgtgtatt ccaccttgac
    18301 ataggaaatg aagcatctgt attctgaggg gtccagtacg aaccaatatt atacaccctg
    18361 tcgagatgtt ctgaaaagtt actcaaagaa acatagcggt tagcattgtt taccccataa
    18421 aattggaaat atacgctaaa acctttccat tcagcaccaa ttgtagcatt ataagtattt
    18481 tgcggacgtt cgggatatcc gtatggaacc ctgtcgtaac catcaacaac accatctccg
    18541 ttaaaatcta tcatataaag gttgcctggc agtttttggg catcataggt attcaatttt
    18601 gtgctactat acaattcgtc ccaggtatta taaaacgtac cattgacatg agtcctggtc
    18661 tggcctatct gcttgccggc agccttcaga taatcatctt tcaaaatagg atcatcggct
    18721 tcaagaactt catcttttga atgtgccata ctaaagtttg cccacacacg caattcgttg
    18781 gcaaatattt tgttgaactt aagttcaaac tcgtatcctc tgacacggac cttgccaagg
    18841 tttgcagttg gaggggtccc accaaaatat gaaggtacag cccggctacc accagccaaa
    18901 agtacatctt tacgagaatc ttgaaaaaca tctacactac ctgaaattaa atctttcagg
    18961 aatgaaaagt ccaccccgaa attggtcttt gttgttgttt cccagtgaat atcagggttc
    19021 ccaatctttg actctgtcca ccaggtataa gggctggtat taccagcctg agaacctaat
    19081 ggagaattgc ccccattggt ccaggtggtc atatataacc aacgactggt tgagatattg
    19141 tcatccccta cctgacccca tgaagctctt agttttaaag acttcagaaa agtcaaattt
    19201 ttcataaaac tttcttcact aatcatccag cctactgctc cagaagggaa aaaggcaaaa
    19261 cggttctccg gcccaaattt tgcagagcca ttgtatgccc cgttaaattc aataaaatat
    19321 ttagatgcat agttgtatgt ggtacggaat acccagtctt cgcggaaatg ggcaaattcg
    19381 ctacctgtag cattcctgtc acggctaaac aaacccatag ctgtaaaatc atgttcatcg
    19441 attttcttag catagtctat ttggccctgg taaaacagtt ttcggtaatt gtcgctacct
    19501 gcaccactcc cattattata tggtgcatct gtattgatac tccattgagt aggcacccag
    19561 tcaaattggt tggtacccaa atacctcgaa taggtaacct cacctgtaac agggtttata
    19621 tatttctgct gtacattgcc attgtcataa atccccccga cagtcttaaa tatgttatca
    19681 aatgatatag atccttttac acttaacccg ttgacaagca tgcttaaatc ttgttttagc
    19741 gtaaaatctg tattcaactg gatggtagat gtttttctaa taccattgtt acccattgta
    19801 gccaatgagt taactgtcga tacttcataa tcttccatat atccccatgc cccatcagaa
    19861 taacgcggca aataagcatc tggcgggtta ctgtatatac tttgccaaat acggtactcc
    19921 catgcatcct ggttgtaaca atcttgtttt attccataag aacctgctaa atttgaagac
    19981 aacgtggttg tttttgttaa ataaaagtcc aggttgccac gtacgttggt tcgtttataa
    20041 ccatatcccg gatcgtatga tttaccattg tctatttttt tcaaaatatc tgcttcattc
    20101 aagaaatcaa ccgatgtaaa gtatttcaca tgcgatgtac ctccggaagc attaatatta
    20161 gcactatacg acatggcata gtctttaatc aattcatcat tccagtcaac gtttggatat
    20221 cgctctgctt cttcctggtt tgccgggttg cgatattttt caagaatagc tactggggta
    20281 tattcttccc atgaagcctc attcatagac acttcacgaa taatagcttc gttccgaaca
    20341 gaaagagcat catacgaatc agcctttgta gccaatttcg aagggacttt catcgtagca
    20401 ttagctgata catgaatttc tgctttacct tcatgtcctc gttttgtggt aatcaatatt
    20461 accccgttgg cccctttaac accataaacc gctgtagctg atgcatcttt caataccgaa
    20521 atactttcaa ttgagctgat atcaacactg ctcattttac gctcaatacc atcaaccatt
    20581 accaatgggt cactgttgtt ccatgagctc tggccacgga tatatatttt tgggtcttca
    20641 tcaccagggg tacctttagt agccaccgta acaacccccg gcaaattacc ggtcaacgct
    20701 gctccaacac tcgaaacccc tccagttttt tgaagtgttt ctccttttgt ctgtgtaatg
    20761 gcccctacaa cgctttcttt tttctgcatc ccgtaaccaa ccactactac atcttccaaa
    20821 tttgttacgc tgacagtcat tgcaacattg acagtcgttt tgtccttaac atctacttcc
    20881 tgaggggtta acccgataaa tgaaatctga agaatttgct cccctgacgt tggaacatta
    20941 atcgaaaatt ctccattttg atttgacaca actcctgttg ttgtcccctt aactactact
    21001 gttgccccaa caaccggcaa gttgtccgga tcaacaatca cacccttcac tgttttgcta
    21061 ttctgcgcct gaagcataga tccataaaag atcaacgcag aaaacaagaa catcttgatc
    21121 agcagcccac tcttcaaata cgcttgaaaa gtttgatgcc gaaacctttt ctttgttttg
    21181 ctcatacgtt tagttaatta aaatagttag taataaaaaa tataataggt ggtaattaca
    21241 caacgaaaat aatcctgtca aaaatcaaca gctttaaaaa aggctttaaa aaatgtttca
    21301 tttacactta ttttctgact atttacaatt tttagacaaa acatgaacga ttttttaaaa
    21361 tggcgcaacg acaggcttaa tgcgacatgt attaaacgtt tgaaaataaa tcatttaaca
    21421 gaaaaatgct taaaatcagt agttaatact atttcggttt agaatttgat gcggttagta
    21481 aaaaaaaact ccaccagcat catatagacc tgacggagtt ataatcatct ttagatctga
    21541 cagtacttgc aatgcctgtc agcattcaaa cttaacaaca ttgaggacac caatattgag
    21601 gttacttcta tccctccaat ggcaattctt ttacaactta tcccttttca aacaatagca
    21661 gagaggctat ttctttatcc tttcaggatt atcagcaaga aattgcttcc agctcttggc
    21721 tttagagcct actgcacctt ctatcatccc attttgatag taatggcaga tagctgccgc
    21781 caaaccatca gtagcatcaa ggtcttgagg tagagttttt aaattaagca ttttctgcaa
    21841 cattgaagcg acttgttcct tggaggcatc gccattaccg gtaatggcaa gctttatttt
    21901 tcggggggca tattctgtta tcggcaaacg tctgaataac gcagccgcta ttgctgtacc
    21961 ctgtgcccgt ccaagtttta acatcgactg tacgttttta ccaaagaaag gagcttctat
    22021 agccaactct gtgacttggt aatggtctat taactcaagg gttttgtcga aaattatctg
    22081 aagttttcta tatatatctt tctctttcga aaggttaatg acatctataa taagcaaaac
    22141 aggagatttc ccatcgcttg taattatccc aaacccagtg atgttggttc ccgggtcaac
    22201 gccaagtata actcgttttt ttaccacact ttacattgtt tacaggctcg taatatcaac
    22261 atttgcaata tttcaataat gcaacccaat cctttccctc acatcatcga gcatttccat
    22321 caatgatatg ctaaacgaca aaggcagcag ggcactttct tttcgcccgg tcaaaagaca
    22381 attggtcact tcctgggctt catattcata accaaaccct ttaacatcaa acttaaatac
    22441 ggtttcagca ccgtcattca tcgtaagggt caaatacgaa ggacaatgcc acatcctgtg
    22501 catctttatc cttccttttg taccgcatat atcagtttca acaggggtat tagccataaa
    22561 tgttgaacat aacgaagctg tagccccatt tttatatgta aaaccagcaa cccatgattc
    22621 atcaactcct gtttcgccta tcacagctga tgttacaata ttttcgggct tacctaacag
    22681 ttgcaatgtg agaaataccg ggtagatgcc aatatctaac aagctgcccc ctccaagttt
    22741 tttattaaac aaacgccatt ccttgtcata taccgcctgg taaccaaaat cagattttat
    22801 ctgcaatata tcaccaatta cccgctcggc aataagatgt tcaactttta aaaatgtagg
    22861 taaaaaacgc gtccagatag cctccatcag aaatatgtct ttctcacgag ccttatccac
    22921 catagcctgc acttcttgtc cattcattgc aaaaggtttc tcgcacagta ccgctttgcc
    22981 tgcatttaaa ctggcaatag tattctcagc atgcataaca tggggcgtgg caatatatac
    23041 aacatcaaca tttgcatctt gaagcatttc ttcgtaggaa ccataagcct tttcagcttt
    23101 atattttacc cgaaattcct cagctttgtc cttttcacgc gaagctactc cgtaaagctt
    23161 cgcattgcag acaaattgca aatcatgggc aaatttctca gctatccggc ccaatccaat
    23221 aataccccag ttgattgttc ccataataaa ttgtgtttaa gtatttcttt tgccttttac
    23281 aaatcccctc ttgatcgcat gattttaaac aactccagtt caatattttc tgtaggccct
    23341 tttgctggag ataaaataag cttaccatca ggcccaataa ggtaataaac aggaaagccc
    23401 cttatatcat attgtttaat aacctccggc tggttgccat aatgaagaaa caaccaacgg
    23461 taatcacttt tttttacaaa atttgccaaa tcttcaacac taccgtctgc tataacgcta
    23521 attatagtaa tttttccggc aaatctttca gacaactttt ttaacaaatc atattcttta
    23581 aaacaagcat aactattgca cgagcaaaaa ttcaaatata catatttccc tttaaaatca
    23641 gcaagttcat gcctcttccc agcaatatca tacagtgcaa aagacggagg tgcataatca
    23701 ggcatcaaac gtgttatttt actcctaatc tgaagcgcat actgtttgtg caaaggcaat
    23761 tttgtacgtg taattaatgt gtctaaaaca gcaaggatac ctgacctcga aaagtttgta
    23821 gaataaaact cgtcatgcag gtttttcaaa attacaagtt ctcttaaagt gtccgatttc
    23881 aaatcattta tcccagccaa agttagttgc aagctatcaa aacttttata agtatttata
    23941 tcttcaaaaa tcttttttgt tactttccct ctgcccatat acacaaaaaa tttggcatac
    24001 aaaaagttaa ataattgcca ataggcagta ttattatata aaactggttt attttcaaag
    24061 tatgtacctg ctacaaccct cgacttttga accataccta tggccattaa atatcctatc
    24121 ctataattgc aatattcttt tacatactca tctttaatag tatcaaccat agcagaaaga
    24181 acatttaatg ccgaatccag aagcgggttt tttcgtacag gcctgacatt ggcagaaagc
    24241 gaccgaaaaa aaggtgcata tttaaaatca aactccgcta tctgccgatt aaccccttcg
    24301 tggtttggat aaatcccaaa aggaagctca acaggctcaa aaaaaggatt gagcttatcc
    24361 tgcatagttt tctcaatctt tttgggcagg ataatccgat aatgtttgcc aggtaccaaa
    24421 taaatataac atttatatat acctaaatat gaatatattt ctttaggctc acaatcgtta
    24481 atctctactc tgaaatagcc ggtatcatca acagctgcat aagctatcaa actgtccttc
    24541 ccggtaaaag ggtctgcaga agtgaaaaaa tcaactcgag tatgggcata ctcgatattt
    24601 accccttcaa taatactttt agaaaaggtg tttaaacaaa aaaaaaagaa aaagtaaggc
    24661 caaactacgc atcattgggc aaagaaaaag tcatttaact cataaatatc agaagtgtta
    24721 agctctgaat caaaatcaaa gaaagaatca atagctttaa gcacttcgtc aaaggaaata
    24781 tatccatctc catcaacatc caacgatttg aacttttcag gaatatccat tgcccctacc
    24841 cttctgccag acccggcaga catattgttt gtaattgcaa gcaatgcttc tacctgatcg
    24901 cgaggtaaag cttccaaacc aagattgtcc cacattaagt tgtctggtat ttcaacacca
    24961 tctttatcta caattgcccc aacagggcta tcattctgct tgtccataaa atcaggcact
    25021 ccatccttat cagtatcttc agggcaacct gcagtatcta ctgctaaacc tttcggggta
    25081 tgaaggcatt tatcattgat atcaaataca ttgtcaccat cttcatctcc aatcaaatcg
    25141 tagtcatatc cttccaaatc aagaaaaaga agtttattcc tcaacatttt tggatcagaa
    25201 aacaagtcaa agtgcaatga aacataagaa taaccaaaat tgtcaaatgc acttccgttg
    25261 tctttaatat tcaaattgta caatgtactt tgtattgggt ttgaagatga tacattatcc
    25321 atattatcag taaaattaag gtgaaaagca taccctaacc ttatactggt acgattagtc
    25381 acatcaaact ggaccccaat ttctaatggt actgcaatgc tatactgatt gtacttttcg
    25441 cctttattta tctcagtatt actgcgcata tctgtttcat attcatcatc aggcttcact
    25501 attgtcgaaa tttgttcagc agattgaatc ttatccctga ttgtcccatc gctccaataa
    25561 taaaacatat tattcccgtt catcaaatta gcttttgagc taaacatgac caattcggct
    25621 ccaagcgaaa caaacggcct gaataaactt ttttgagaaa taaaatgatc aaaatcataa
    25681 tgggcattca acccaaatgt aagcaaatct gaagtaaaac gaaaattttt agaaggatcc
    25741 gtatagtccc tttggataac ggtcatagat gttttactat ttcccgggac agtaaataag
    25801 gtataaaaat tgaacttgta atgcctttga gcatcaatat atgcatgcat attgactttg
    25861 aacgccaggc tgcctgcaaa aatagaagaa ccattatttc tgatatcccc catataattg
    25921 atataccccg ttccaaaacc aataaccggc ttatatacag ggttaatatt ttctacatcc
    25981 tggataagca tcttttccca atccaactcc tgggcagata aaaatgcagg aacaacaaat
    26041 aatataacag caaataatat tttagatata ttgttcatat ttacctatac caactctggt
    26101 ttgttatcat taacgcttcc tgcactgtaa tgcgtttgcc actattgtat gccgaaacaa
    26161 aggcatctgt caaaggagtt gtattccaaa tatgaaccct gtagtctcga gcttgtttat
    26221 attcagcaaa agagccaata gagtatttac gccatccttc atgttcttcg gcacgtacct
    26281 catcagcaat tttatatctc ttaaaatacg ctttaatatt aataggctta tgcccggctg
    26341 ctacctgaac cctgtaataa ataccttctt gtgcttctaa ttgatccgaa gcattaattg
    26401 acgaaccggt ttttttctgt acaggctttt tttccaaagg ttctataact tttttaacag
    26461 gttcttgacg agctaccata gcacaatctt taggctcaac tttaggctga acggcaatta
    26521 attctgaaat aggtacctga acattgccta cctttgattc ttctataagt ttctttatct
    26581 cttcttctgt taatgaggca agcatcacat ctttttcaat aatatctttt acaatcgtgc
    26641 gatcgtctga catatacgag aattgcccct tcaaaacagg agcttctata ccagccttgt
    26701 taggcaccaa tcggtacgaa actataaaat aattgttaga tggaagattc atccacataa
    26761 tcttggccct cccatcttta taaataaaca taccttcttt actatcaata cgcaaagcag
    26821 tatagccctg tggtacaatc tcttcaattt tggcaaactt ctgcgcattt tctttataaa
    26881 ccaaaaggtt tacaatataa ctattatcgg catcattagc cggaacaggt ttttccctga
    26941 aacaagccac attttgttct ttacctgtag gcaattcagg tattaccttt tctttgaaat
    27001 ccataatatc tactatcaac gaagggtcta tattggggtt ggggttaata gtaattggca
    27061 gcaactccga ctctaccgac atccgctcat tattgtcaat atacgaaaac ttacccccaa
    27121 ggtcaaaact tcctttcaac cgcggatcta ctttgatggt ataggttatt tctacagaat
    27181 ctgtttctgg catcttaagc caaatcaacc taaccttatt gtctttaaac gtaaagtcgg
    27241 cattagccga atatgccgca gttgctttca atcccatagg cagctcttgc aaaaaacgcg
    27301 aaaaactttg caccttgccc ttctgaacaa caacctttac ctgaaattca tcaccagctg
    27361 taacagattg gggggcagta acccgtacat taacgtctcc atcgaacata aggcctgcaa
    27421 acagactaat aaggacattt acaggtatta tcaaccattt taacatagtt cgactcttta
    27481 gtaacgatat ttcctatata tatataacat tagctactcc ttttcaccaa attgacaaaa
    27541 caaatttaat tatttacaca tgaaaacaag cagtttgtta atattttctg caaattgttg
    27601 aaaacttaac tcaaacaaaa acacgttatc gtattgtgaa tcaatatttt attcttttta
    27661 aaaattaaaa gtttggactc aataaatatt tagcatagaa actgttgata atttcaacgg
    27721 cttcatcggc tgtatccaca atggtaaaaa gttccatgtc agcaggacta acattacttt
    27781 catcttcaag taacacttct cgtatccaag ctacaagtcc atcccaataa tcttttccaa
    27841 caagtactat cgggaaacgt ccaattttac cagtctgaat aagagtcatt gcctcaaaaa
    27901 gctcatctaa agtaccaaaa cctcctggaa gaacaacaaa tccctgtgca tacttagtaa
    27961 acataacttt gcgaacaaaa aagtggtcaa aagtaatcag cttatcgtta tcgataaata
    28021 agttaggctc ttgctcaaaa ggcaaggcta tattgatacc tactgaactt cccccacctt
    28081 ttttggcccc catattgccc gcttccataa tgcccgggcc tcccccggta ataataccat
    28141 atccatattg tgtcaactta taagcaatct cttcggccaa cctgaaatac ttgttatcag
    28201 gctttgtcct ggccgaacca aatatggaaa cacaagggcc aatacgtgct aatttttcaa
    28261 aaccttcaac aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtaggcgt
    28381 ttttaaccgg gtatgtgcaa ccatatcaac aacagagtca tctcgctcgt gcccaatacc
    28441 agaaagtact ggaattggaa attgtgcaat atgcgaagcc aacaaataac tatcaaagca
    28501 gctcaggtca acctgcccgc ccccgcctcg aataatggca acaacatcat acttttctat
    28561 ttttgcaaaa atagcatcta aagctgcaat aatagatgcc tcagctttct cgccctgcat
    28621 taaagcctga aacagtgtga cttcaaacct gtaaccatga ggattttcgg ccaggtgggc
    28681 cataaaatca gaatatcctg ctgccccttc agaagaaact acagcaatac gctgtacaac
    28741 aaggggcatc tggactattt tattcagttc aaaaaccccg tcctctttca gctttttaac
    28801 aacctgcatc tttttcagtg ccaggtcgcc aatggtataa ccagggtcaa tgtcaacaat
    28861 atgaaaactt agcccataaa gaggatggaa ctgaacagaa gctttgaaaa gtattttcat
    28921 cccccgctcc aacctgcgcc ctgtagccgt ttcaaaataa gcctttagca tcttatagtt
    28981 gcgcgaccat accatggcct tggcttgcgc caaaactgta tccgaaactg catctttctc
    29041 aaccaattcc aaataacaat ggccggaaga attctcagat atacgggcta tttcagacac
    29101 tatccagtaa ttgttggaca ggttatctga aattacgcca gcaatcaggc tgtttatctc
    29161 atagaggcta aatatcttat tttccatcta tcgtttttta acgccaacaa aattacgtct
    29221 ttgcccattt ctttcgattg taaaaatata cattccttcc cttaaatact gcaattctcc
    29281 ttgtaagtgt ataaagtctt tttcattttg caatgaaaac atttgttgtc cgtacaaatt
    29341 aaaacactta agcgtagtaa tcccgctgtt ttcaaaatag gtattaatat tgagcaatgt
    29401 gctaaaagga ttgggataag ctaaaaaacc tacatggttt gcattgttct cccgggcaag
    29461 tatttggtat gccttataaa aatcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29581 nnnnnnnacg ttggcccgac agatgaaaaa acagctctaa caccggtgct atctacagct
    29641 cccactgtaa ttaccttttc agcatcggca gggaccccga tgtatttcca tgcattattt
    29701 ccttcattgc cggcactggc aaccacaatg ataccctttg aagcagacat ggttgcccct
    29761 tgagatatcc ttgaaacctt gccatttaaa tcattataag aataatcaaa ccccgtatca
    29821 tcaaaagtgg tgtatcccaa cgaagtgtta attacgtcaa ccccggcact atcagcatat
    29881 tcagcagccg caacccagtt gtcttcttcg caaggccact cgctctctgc atcctctgtc
    29941 ctgaaaagat aataagttgc ttcgggagct gtaccataca aaataccgtc tttttcacca
    30001 gccataatac tcaaaaccat tgccccatgt gtattacatt tatagatatt tttccgggga
    30061 taaacaaaat ctgctacatc agcaatacgg ccatcatttc gtacatttgt aaggctctca
    30121 taaacatcgg cgttaccaaa gcctgcatcc ataacagcaa tgctaatgcc tttgccttta
    30181 tatccaagct tatgcaggct aaatccatta accaaagcaa cctgtgcaaa gcttatctca
    30241 gtataatcgg ccgcatcttc aagtttcttc caatatcttt tacttgtaaa agtagatttc
    30301 tttcgaataa tccgggcctc taaaacacaa gatatttttt ctacttcaga tatattattt
    30361 ttgcccaggt caaccgttac acaattaaac cactttgatt tgttgacgat tttaatccct
    30421 tttgaggtaa gactgtctat ataagcgggc ttaattggta aatcagtttc atccactttt
    30481 acattatttt tggcccttcg ctgcaatgct ttatttgaaa ggaataaatt gggagagtct
    30541 aataaatacg cctgtcctgc tttatcttta aatgtaaccc tatacaactc aacatcctga
    30601 gcccgtaatg aattggttgc aacaaaaaaa aacaggatga tatatatggt atattcaaca
    30661 atactatcgt ggcaccatat tattttcatc aggttcggga atgaggcctt tgtttttttc
    30721 aatcatttca aatatcttac gttgattttc ttctaactgt gaagcatttt gaggtactcc
    30781 attaatgtat aaaatttcca ttttaacgtt gcccttttca tcataatagt tccatttgcc
    30841 ctgcatcaca ttgttctcaa actttccttg catctcaagg ctaccatcag gcctgtacat
    30901 cttgtacatc ccttcacgtt tatcattttt gtaaaaacct tctagcttag gcaaattatt
    30961 ctcaaaatac atcagccatt caccgtcttt aacgttattt ttccaaacca accgctcagc
    31021 aacatttcca ttattgtaaa tacgcatagt gactccattc tttttgccta aagaataagt
    31081 ttctttcatc ctcaaggttt tgtcataata agaaaaatat tgccaaacgc tatctttcaa
    31141 agagccaaca tacttcccct ttgcagccat ctggccattt tcgtaataaa aagtggcata
    31201 agaaatagta gtattgggta caaaatattg ctttgccttg attgtcccat cttcaaaata
    31261 tcttacaaaa tctccaacag gcttattgtc tttaaaaaaa cccttgtatt tgaatttccc
    31321 gttttcataa aaacttttcc agtgaccttg cttcatccct ttactatcag ttgtattcca
    31381 aacagtatca gactggcagc ttgcagcaaa caaatgaaaa aacaaaagat aaacaatcag
    31441 aacacttgta gaataagctt ttattgacat aactgtattt gttaagtaga acttaacaaa
    31501 gtaagcctat ttttaacaaa ataacaataa agagattgct gccaaactaa tttttaagct
    31561 ttttataaat atttttcctg tgagtgttaa cagtatgtat gcttaaccca agctcatcgg
    31621 ctatttcttt tccgctcata ttatctttaa gcattaacca tacctgggtt tccttttttg
    31681 ttaatatgcc attctcgtat tcaactataa aatctttttc aaatactttc ttttcggtgt
    31741 tattaattga tttataaaca gtcagtgtct gaatattatc tttagggata ccttcacctg
    31801 ttttaaaaca caaaccaatc attgaggcta atatttcatt actctttttc tttaaaaaac
    31861 gtaaactaat attaagcttg atataatgtt tgtttttatt ctgaacccag caatgatacc
    31921 taaatatgta ttttgatatc tgcgaagcag aaagctcttt gacatatttt tctatatgat
    31981 tatagatggc tttttgagta ttcaaaaaat ctggatgata agaactttca gcaagcccca
    32041 acatattaca atcagcccca ttatatgggt tataaccaag cacagtgtaa accgaatcac
    32101 ttataaatga atatttcttt tttgcataat catagcaaaa gtaataaaat ggcccaagct
    32161 tagacagtgc atcaggttga atactacatg acagattttg tccatagccg gaatttccgt
    32221 ccagatatga cattacatca ttttcgggtt ctaaatcatt tttcataagt aagagtttac
    32281 ttacaaatat aataaaataa ttagataatt aaaaattatc aaacattttt tattacattt
    32341 atatcgctaa atatcaatat tttaattttt tattttcaaa atactttgaa ataaaaagaa
    32401 ttttaagcag caaataaact caatgaaatc aattattcta ataatgaaac gagcataatt
    32461 cgctaatcac aaacatggat aaaaagaaaa tcaaatcgtc agtttttcaa aagtttttca
    32521 gcagatgcca tgttcaagct attgcaaaca ttcttagctt cgggagtgta taaaaaaatg
    32581 atgagcttct cgtaacgttc aacgacctta ccgcgaacca ttttcaatgt actgttcagc
    32641 atgtggtttt cttcggtaaa aggttcattt agtacgccaa tggcagccgg caaccaacgt
    32701 tgcgggaaca tgtgcccaaa ctttctccca gttcgatatt cgttaacttc ggattcaata
    32761 agcttaagta ccgcttctgg cgtaggagta tggtgatgtt cggataattt acgctttatg
    32821 gcaactatat tgggcactat aagtgcaact gtatatggtt tttgattatt atacaacata
    32881 cattgctcaa taaaagaaga ctgcgaaacc atggcttctt ctatcccttc cggactatat
    32941 ttttcaccat catcagcaat caaaaggctt ttaaaacggc caagcacata taagaaatta
    33001 tctttatcaa ggtaacccat atcgcctgag tagagccaac cgtctttaat cgtttgccgg
    33061 gtagcttctt cattttgcca atagcccgac atcacattgc caccttttat aactatttct
    33121 cctttttcgc cctgcggcaa aacttgtcca ttttcgtccc ggatagtaaa atccatatta
    33181 gggacaagag aacctgatga acctaattta tgttttgtaa gtgaattggc cgaaattatt
    33241 ggagaagcct cggtcaaacc atagccttgc aacatgggaa tgccaatggc ataaaaaaac
    33301 ctttgcaatt caatgtcaag taatgctcct cccccaacaa aaaactgaag ccggccccca
    33361 aagttttctc ttatcttttt aaacaaaatt gcatcaaaca acctcaccgg caaataaaac
    33421 aataccctcc aacccttacc tttattgtat ccattgccat tataaaggta tgctgttttc
    33481 agcccggtag taaaaagcca ttgaacaata acccctttag cttgtatagc agattcaatg
    33541 ttttttcgga aatttttggc caaagccggc acgctaagta aaaaatgagg gcgtacctct
    33601 tttatattga ccggaatatt ttttaacgtt tccaaaggcg ttttaccgct ttgcacagca
    33661 gccaatgaag ctccggcttt tgcaacagta taaatacccg cagtatgggc aaaagaatga
    33721 tcccagggta aaataagcaa agtaacaaaa tagcttggaa cagctataag ccctaaagcc
    33781 tgggtggtat tgcaataata gttgccatga gacaatataa tgcctttagg atcagctgta
    33841 gtccccgaag tataacatat agtagaacta tcatcgggca aaatagaagc tattcttttc
    33901 tctaactcag attgtatatc aattcctgca tttttgccca tgcagacaac ctctttataa
    33961 cttatctcta aagaaaaacc taacggctca tcatcaacaa caatgatttt ttcaatgaga
    34021 ggaagcgatg gcaataacga aacaatcttt tttgcctggt aaccagaggt aatgacaaat
    34081 tttgcccccg aatgttttaa cctgaaaagt aactcgtcag gttcattgag cttaacagac
    34141 aaaggtacat taacagcagc acaatacaat atccccaact ctgaaacaac ccattctgtg
    34201 cgaccttcaa gaagcatagc aacgcgttcg ccacgtctta ggcctaagct cagaagccct
    34261 ttagcaaaaa aaattacctg ttgatgtatt tctgcataag taagggactg atattggtct
    34321 tttatttttt cccacaaaaa agggttacca ctatattttg caacgctatc ttcaaataat
    34381 tgtactatag tgcccatgca ataattactc tattcgttta agtttggtat aaaatttccc
    34441 ttgggtgata cgggcttttt ttgaagtatc agccggaata acacctacga actcaaaata
    34501 gccttggagc gtattgtttt caacaatttt gtatatcttt atttttcctg aagtcccttt
    34561 tcgaccatct gcccaataag tagtatttct gtctgatgaa taattggcct tgataaaacc
    34621 aacagattct atcaattgtc caataggata ttctttgttc atcttaagct caatatcact
    34681 catttcaata acaagggttt gattttgcca ggtaccgtta attacatatt tatctattcc
    34741 attgcctgat atgtgggtag caactgagtt ataagacctg agcgtatcat taaccctgaa
    34801 agtcatggat gcaggcttaa tatcaatatt gggaatacgg tcaggatcaa acaataccat
    34861 gttcaggttt atttcacctt cacattcaag cgtatcaata ccgttgataa ggtctgtttc
    34921 ataagttaca aacttgtacc gcgcttgtaa aacactacct ttctgatact tggccacttc
    34981 tactgttccg gccgaaaatt catgttctac atttgactca tccctataag tagccctaac
    35041 ccctgatgta tcattaatcg aagtaataac ctcataggaa gtattgctca gattgacaat
    35101 tttatagaaa gtaattttta aagctgtttt caagctatca tcaagagcat taaatactaa
    35161 ggtagtagta tctccaccat aaataacttt ataatcttga atattataga ctaaactggt
    35221 atctgcatct ttatatgtga ttgtattttc atcagcgcca acactcgacc ctcctccttt
    35281 atcgctacac gaaaccagaa ttaaacaagc agaaaaaaac agaacaatta gtttggagta
    35341 tttcataatc attttttgtt taaatttcat cacaaactta aagctttttg aaaataaaac
    35401 aaaagataag cgaatgacat tgcttatctt catgtttgtt ttttaattaa aaactaataa
    35461 aacaaatatg aattcacatt tttatttata aaaaataaaa aataaaaaag aaaattatac
    35521 tgtactctgt taaaaatttt ctcacttttt tctaacttta acaaaataaa ctaaaaacaa
    35581 tgaactcaaa agtcaaatta ctaatagttg acgaccacga cattttcaga caaggtgtta
    35641 agatgctgct caacaatgat tctgctgtgg aagtaatagg tgaggccagt aatggaaaag
    35701 aatttcttga cgttgttgaa aagcttgccc cggacgttgt attgatggat attgccatgc
    35761 ctgttatgga tggcatagaa gcttcgaaac tggctcacga aaaattccct aatctgaaaa
    35821 tactggccct gacaatgttt ggcgatgaaa aatactatta tccgatgatt caggccggca
    35881 taaaaggttt tatattaaaa tcatcaggca taagcgagtt gcaaaacgct ataaatatga
    35941 tttcgcaagg ctttaactat ttttcaaacg agttactttg caaacttatt aagaatgtaa
    36001 gcgagaaaaa tagtgtccct gatgataaaa atgataagct caccaaacgc gaattagaaa
    36061 tactcaaaga aatagcttgc ggtttatcta atgatgatat tgcccaaaaa ctacatatca
    36121 gcctttcgac ggtaaaaacc caccgcaaca gcctgcttca aaagaccggc actaataata
    36181 ctgccagctt gattatgcat gctattaaaa acaaactgct cgtagtagaa gaataaacac
    36241 ttggcttaat atatcctcaa gagcttatag caaaccaatg ctataagctc tttttgtttt
    36301 ctgataacct aaagctgtat tgctgcatcc acctttctac cgagccaaaa atcaaccttt
    36361 aggtcaggat aaatacaccc aacacacgat gcattgagga gccttcaaaa cctaaatttg
    36421 ttaaaaaact cagtctatgg atccaattat tcaaaaattt agggagaaat tctttgacga
    36481 ggcaggacga ttgctcgacc aactagagaa agatttgctg gagctcgaaa aagatttatc
    36541 caataaggaa cttaaagagt ctgctttcag ggcaatgcat acaataaaag gtgtcagtgg
    36601 catgtatggc tttgatttta tatgcgagtt tactcatagt atagaaaacc tttaccaagc
    36661 tcttcgagaa aacaaactta atgtcgaacg aaacttcttt gacatctcgt tttcagccat
    36721 tgaccacata agaaaacttt tggtagatga aaaactcaac gaccctacca accagaaaaa
    36781 ccatcacgac ctcatcaacc aggtcaacca aatagtgcag actgcacctc caaagcccga
    36841 accaaccatt caaaaagaag aagcaacaac aaacgagaat actgaaaaag ccagtacatg
    36901 gcacatattg ctaagaactg atgaaaaaat gtatttcagg ggaatcagtt tgctgaacat
    36961 tttcagggaa ctggctacca ttggcgaatt tcagattagc cggttagact ttatgagcca
    37021 gactgaatct gacacctgga gcattatcct cacctccaaa gcgtcgcagg atgaaataac
    37081 cgacatattc cttttcattg aagacaattg cagcattaca caacttagcc tggaagatgt
    37141 aaacggcatt tttgcccatc agaataccga agcccaagaa gaaagcacag accatgaaat
    37201 ttcaataata gaatatattg aaaatcaaaa caaccaaact aaccaaccta aaaaagagat
    37261 ttcttcagaa gttaaagagc aaaaggctgt aaaacaaaaa gaggccattg agcgaaaaag
    37321 aatatctgta gattcgcaaa agctcgatca actgatgtac ctcgtaagcg agttaataac
    37381 cgtcaattcg cagcttgaac aaagcacaaa agaagtaacg tttaattcca ttcgaggatt
    37441 tttagaaaaa gtggacaacc tctcaaaaca atttcgcaac aatgcacttg aaataaggct
    37501 tgttccgctc agcgacacca tcttgcgctt ccaaagactt atccgcgatt tatcaaaaca
    37561 aatgcacaaa aaaataaacc ttgaaacatc aggcattgat accgagcttg acaaaagcac
    37621 aattgacaac ctcaacgacc ctttaatgca tattatccgt aactgcatcg atcatggcat
    37681 tgaaacgcca gaacaacgca tggcaaaagg aaaaccagaa acaggaacaa tcgctatcag
    37741 tgcatatcac tcaggaaatt atatttatat aaaaatagcc gatgacggaa gtggcattga
    37801 cgctgaaaaa gtcagaaaaa aagccattga caaaggcatt tggaaagcag actatcagcc
    37861 cacccgaaaa gaaatcttcg accttatatt cttgccaggt ttctcaacag ctcaaagtct
    37921 taccgaaatc tcagggcgtg gcgtaggcat ggatgttgta cgcaaaaaaa tccaaaacct
    37981 caggggtgat gtcgaagtcg aatcagaaca aggaaaagga acggcattta ttctcaaact
    38041 acaacaatcg gtaaccatca tagacacctt gctctttacc gttaatgaaa gcaatttcat
    38101 tattcctatt tccgacattc aaatttgcag tcaatatagt ttaaacgaaa taaacttgcg
    38161 cagacataca gcaacactgc catataataa tcgactcata ccatttattg atatgcgtca
    38221 atggtttga
//
LOCUS       DAOSPF010000034        38009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1118_length_38009_cov_6.727275, whole genome shotgun sequence.
ACCESSION   DAOSPF010000034 DAOSPF010000000
VERSION     DAOSPF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38009
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1118_length_38009_cov_6.727275"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..903)
                     /locus_tag="PK252_08230"
     CDS             complement(16..903)
                     /locus_tag="PK252_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14736.1"
                     /translation="MDVKTLKDRLVNAYAVDNLNKIALVLINLYQSQQYSVLQKIAEM
                     IGDYIDIEIDDKGKGFSKLMMLYHPDKAAFHISEIEKLANANDFDRLLDYSHILRLEK
                     IEEIAQSLDSFEDIDYSPVYGWDTEAEGFRIVNDYEKKTTFQQQNYTKNDGLTFYDAI
                     KIRYYGHTEIEYPTYYLEDIDEFELSASNINDLEGVQYCKHVVKLDLSANSIADIDLL
                     CSLQNIEELNLSDNEIHDIAALSNLSNLKSLFLNNNNISDLSPLFELTELELVEVLDN
                     PVNALHVQQLQELGVNVVC"
     gene            complement(1001..1456)
                     /locus_tag="PK252_08235"
     CDS             complement(1001..1456)
                     /locus_tag="PK252_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14737.1"
                     /translation="MEKVSVSVVKKVIVAFSITVIWAMYNVKAQELLAYSDLKVSNSE
                     IVNGKEELKVNKQLAEIGAIIGQYIGKEAEMHLDQYNITAGKYYCDIDLWVSKTGKIL
                     RYELNNKVEPKIDKMLADIISKAPVFEAVKMNGFAVRMHYQIPVTIIVR"
     gene            complement(1974..2738)
                     /locus_tag="PK252_08240"
     CDS             complement(1974..2738)
                     /locus_tag="PK252_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT14738.1"
                     /translation="MKTIIIEDEELAARRLESMIKTIDPQIEVMAKLESVEDSVAWLK
                     TNSHPDLIFLDIHLEDGISFEIFDQVKVNVPIIFTTAFDEYAIKAFKHKSIDYLLKPI
                     VQDDLGKAIAKYREWTGTQSAQLDLTGLYKLMQGNNKEYRERYSVSVGEKLKTIEVKD
                     IAYFFSTSGITFVVMNSKNQYSLDLSLDKLYNELNPKDFFRVNRQYLINHKAIANVHV
                     FPKSRLKIDLNPPCQEDLYVSLDKVAEFKRWLDGEK"
     gene            complement(2760..3170)
                     /locus_tag="PK252_08245"
     CDS             complement(2760..3170)
                     /locus_tag="PK252_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14739.1"
                     /translation="MKIVIIEDEAFAARRLENLVKDYNPQFQIVAWLESVQDSIEWFS
                     QNTHPDLIFLDIHLEDDLSFAIFNKVTVTCPIVFTTATDELAVKAFVTKGIDYLHKPI
                     VQDELNKMLDKYSSGDFPQRIIDAQYFNDLFNTK"
     gene            complement(3220..7167)
                     /locus_tag="PK252_08250"
     CDS             complement(3220..7167)
                     /locus_tag="PK252_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT14740.1"
                     /translation="MNLKELNELNKKESFKDFRLKSPRQLLLALTLGGTIIVALWQFF
                     DGMYGIHQSKVLFQLRGLTLAAMLLNLLVGLIRANDKAYKQHIIFGFYIGTLFCTLLA
                     LLTGAGQSPYWYGLFFVLIGWFILLPFSYTALTFNGLAFVAIFTGVIFTQHKDFIDAQ
                     VYFRIFSIYSGILMVGWIAAINRNKADAENFLSSIRIQEANEELQAAIEELRAEVESH
                     HETTCKLQEKQQLLDSILENAPMVIWSIDLEGRFIYSQGKGLENIGVRAGDNIGKSVF
                     DVYKGTEAEIFVRKVLDGTIEQDIVKVKNLLYDTRVSPLYNTNGLKYGYIGVAMDVTE
                     QKMAEYNLKKFRLVLDQVPVAAYIIDNNMHFEYVNAEFAKQSGYTVEDLLHKHINETI
                     YNGEIPASRKFIAEALLRGESWQGELYSRHKTGRMYWANTIASPYNDEAGNVDGYIVI
                     QQDVTDQKQMELDLIESEALYRSLIENTLDSVVLTQDYKFIYVNDVFLNLMGYTHEEL
                     MNIEPTSIIAPEDKERVMEYHAKRINGEVKAQTYTATFVRKDGSRFIAEMNSSTVVIK
                     GKNSSLVTLRDITEREKLQQALRESELQYRNVVEQAIDGITITQDRNLIFANKAFCDI
                     LEYSMDEVLGMPVMQAVSAEDMDDMNDMFRRIESGEKFTRYTRWNAIAKSGKKKSVEL
                     HVTVIDYKNRPAFLVITRDITERLKMEQAIHESEAKYKTLVENSQDCITIIRDNKFLF
                     VNDTYCKMLGYTREELYNMPSVATLHPSEHEKAFAIAKRRNELDFSTINEIFLFLTKD
                     GQIRECETTSTLIQYQGEWASFFTSHDITESKRLQLAIQENEEKYRLLFEAESDAIFM
                     IDVETGDILDTNPAATRIYGYSKEEFLKMKNTDVSAEPERTKTATNNKETLVPLRYHK
                     RKDGSVIPVELSAGFTVLNGKGIQIVTSRDITEREQNQKALAESEEKYRELVETTNSI
                     ILKWNNKFEITFLNEYGLKFFGYQKEEIIGKPIVGTIVSPSEQYTGRDLKQLMEDVFK
                     TPEKYAHNMNENTCKNGEKVWISWNNTAIKNLDGEVTDMFSVGTDITERRRNEEELRI
                     TKEKLQELNQNLEIQVNEAVKKLTEANTQLIRLQKENLQSQFEVLRQQVNPHFLFNSL
                     NVLTSLIKLEPDLAEKFTEHLSKVYRYVLENKDNDLVSLQTELDFLDAYLFLLNIRFM
                     DKIKVEINIEMDCKAYLILPLALQLIIENAIKHNTMSKKAPLVIQIFADAQQMLNIIN
                     NLQERESHMASTGVGLKNIAHRYELLELPAPQFEKTETHFIAKIPLKKTEQIL"
     gene            complement(7239..7670)
                     /locus_tag="PK252_08255"
     CDS             complement(7239..7670)
                     /locus_tag="PK252_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT14741.1"
                     /translation="MDYRNMTAPCGKDCFNCPLYIGEENKENRREFFERGNIPDEKYR
                     CLGCRDNKGYCPGLEILGIDPHCKTYQCVQEHKVEFCFECGQFPCFRLQPVADRAERV
                     PHALKVYNLCLIKKMGVEKWAEELSKKIFSDYYTRKLNDFM"
     gene            complement(7923..10373)
                     /locus_tag="PK252_08260"
     CDS             complement(7923..10373)
                     /locus_tag="PK252_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14742.1"
                     /translation="MKTNNLLRLFTITSCMAIAFAIGVNAEPMAEMPFNSVNGKIVDE
                     QSGSPIEFASVAVFNTSDSSLVSGTMTDENGNFKIKDLKIGSYYVKATFIGYDEYRSD
                     AFSVSKAGKSANLGDIAMKSKNTKIDEVVVKGEKSRIEYKIDKRVINVDKDLNAKGGT
                     AANALENTPSVQVDPQGNVTLRGSSEFIVLIDGKPSVVKGSDALKQIPASAVKQIEVI
                     TNPSAKYDAEGQVGIINVILKKDKLQGLNGNVSASAGTHDKRNGNVLLNYRAGKANFF
                     GGVDFIDNKYSEVIDFNSRITQNNASLHTLQSTDQFNYNNKLSFKAGIDYDLDTMNSL
                     SFSGTIGREGYDNGSNSQARVIPDIVDNTTYFSSNNYMDVMGDVYSLNADYSHSFTKD
                     SKISFTNIFYNWKGEDDNKLNELNTNSVYEYIDVSSMLHNHKDNKNYNYRANVDFSSP
                     LLSGNFEAGAQFRYEYRWEDLYFENYDVNQALWVKNDTFSNNLKYNNTIYSGYTTYSG
                     SVWGINYQLGLRGEVFLRKIEISTDADPFEYNKFMFYPSVHLSKSINEKHQFQLSYSR
                     RINRPQPWILSNNPSYLDSKNVFIGSPYLKPEYTDAYEFNYRTTLKIFTFSTQTYFRN
                     TTNCFQTSRYLNTDGKMYHRLINSDNQQSYGVEQGIDINLSKWWQINSGANVYHYILK
                     TMNGASENKKEVNTWDARYVNNFSTKWKTRIQTVFYYRAPSIDAVGDVNGFYTVNMAI
                     SQSILKDRGNISLSAQNIFNSIKFDYKVRGAGFDNVYGINAEGTTFMLNFSYSFNNFQ
                     YKNRGRADALEFKGGGAF"
     gene            complement(10684..11043)
                     /locus_tag="PK252_08265"
     CDS             complement(10684..11043)
                     /locus_tag="PK252_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOT14743.1"
                     /translation="MQLTARLINVLPLQTGNGKNGQWRKQDIIVETDGQYPKKVCIAI
                     WGDKINESQLQVGNLLKIDFDVESREFNGKWYTDCKAWKIEIAGGSQSEPSDWQAAPI
                     TEEPPMGVGAPVDDLPF"
     gene            complement(11063..12586)
                     /gene="dnaB"
                     /locus_tag="PK252_08270"
     CDS             complement(11063..12586)
                     /gene="dnaB"
                     /locus_tag="PK252_08270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT14744.1"
                     /translation="MAKQYSNRQSISTPVPVSELGKMPPQAIEIEEAVLGAIMLERDA
                     IIAVMDILRPESFYLDKHQKIYKAAMELSRKEKPIDILTVTEELKKEKHLDEIGGAYE
                     VSSLTERVASAANIEYHARIIQQKFIQRELIRISSEIQTRSYDDSIDVDDLLDFAEKE
                     MFDVAQGNIKKDAVEVSSLISEALHQIEEAGKNTNSLSGVPSGFTKLDAMTAGWQRSD
                     LIIVAARPSMGKTAFVLSMTRNMAVDYQKSVAIFSLEMSSLQLVNRLISCETELPSSS
                     IRTGKLSTEEWQQLEAKVKPLEKSKIFIDDTPGISIYELRAKCRRLKKLHDIDIVIID
                     YLQLMTGPPETRANREQEVSVISRSLKALAKELNVPVIALSQLNRSVEMRSGNKRPQL
                     SDLRESGAIEQDADLVCFIHRPEKYGITEDEYGPTIGIAEIIVAKHRNGAIGDVRLRF
                     RDFIARFIDLEDVLPYDDNIEVKPIKTVGSKMNDDLSGVDMNSLPVNNNFDIESPFV"
     gene            complement(12862..13173)
                     /locus_tag="PK252_08275"
     CDS             complement(12862..13173)
                     /locus_tag="PK252_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOT14745.1"
                     /translation="MAENIEKLHLDDADYITTFTRKFRNRKLYEPVNPKLITSFIPGI
                     VREIKVNVGQVVKKGEPVLIVESMKMLNIIRTPVDGTVKSINVKIDEKIAKNHLMVEI
                     A"
     gene            complement(13188..14732)
                     /locus_tag="PK252_08280"
     CDS             complement(13188..14732)
                     /locus_tag="PK252_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT14746.1"
                     /translation="MALEKKIRELKEKREIVSKGGGDKAIEKQVAMGKLTARDRINSL
                     LDKESFHEYDLFVQHDARDFGMEKKELHGDGVITGTGTLNGNPISIFAQDFTVVGGSL
                     GRMHAGKITKIMDHALKMRMPLIGINDSGGARIQEGVNSLAGYGEIFFRNTLASGVIP
                     QISVILGPCAGGAVYSPALTDWVFVVDNISKMFITGPEVIKSVLGEEISQEDLGGARV
                     HAEITGNAHFFAKTEYECFEQIKKLLSFIPWNNSEKAPKTAPKPPKKEYKIDTIVPED
                     PKQPYDVRNVIRAIVDDSDFFEVQEMWAANAVIGFGRVNGETVGFVANQPLVMAGVLD
                     VDSSDKIARFIRYCDAFNIPLVTLVDLPGYLPGIDQEHAGVIRHGAKILYAYSEATVP
                     KTTVILRKAYGGGYIAMSSRHLRADFVFAWPGAEIAVMGPEGAANIIFRKEITEAADP
                     EAMRKEKVKEYSEKFANPFVAASKGYIDSVIEPGETRRYIIHAIEVSERKDISMPKKK
                     HGIPPF"
     gene            complement(14943..16313)
                     /locus_tag="PK252_08285"
     CDS             complement(14943..16313)
                     /locus_tag="PK252_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14747.1"
                     /translation="MRTNLLFKLAVALFCVSCLVGCEDNTKDAEAGPTLTVSGSNISG
                     NSINASAGTSITVKWVAEKGTSDLSTFSIKAGAGFLDSWQDKTIDQVSAAKYVDSLNI
                     QLPQTEGEYLLSFVVTDKKGKTASQSLTVFLTKPPITDVFAYYQNSTFIFVIKDGTST
                     VKTAKMTWKVTNYNESSKVATISCTLDPATTVTPLNSVFYFRKATSGALEFSNDGVTW
                     RNLTDNSKDINFIFGTKAAKPSSLYGSVNTTISSAYVIYPEGTSSGYLVSSEYNSSNA
                     DRYLFTEYTKEYYSDKVGFTQFVTFTSDGSDYPPFTYKREIELVYYKVYQSGGTIIEG
                     GAKKPAAPGNLSGSYARRVESWNSRTGMYELHSFISLSWTDNSDNEIQFNVYIKADDG
                     NYYPLSSITNLVLIPDYFPANTNSGIVRRGYYVDWDAGTYYFKITAKSATEESDFSNE
                     AAVTCY"
     gene            16521..19628
                     /locus_tag="PK252_08290"
     CDS             16521..19628
                     /locus_tag="PK252_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT14748.1"
                     /translation="MKITNKVYSKTSAFLHHSFVLSILLGLFLNANSQTSISDVKPDN
                     FSNALALFKQGKYAAAQAKFESIANNDVSKSNDIKREALYYSFLCSQQLLLNDAENKG
                     LEFIKTYGGSSKANDVYFQLGLNQYVWKRYPKAIDYFAKVKKSELTESQQSEYIFKYG
                     YSFFMLDSLEKARKQFYELIDIDTRYTPSAIYYYAHIAYTQKNYETALQNFLKIKDDE
                     MFAAIVPYYISQIYYIQEKYDALIEYAQPLLDSVIESRAAEMNRLVADAYYQKKNYDK
                     ALTYYNVFLSKKSDLKAAEYYQPAFCYYMKSDFQKAIPLFEKAALDDSLTGQYANYQL
                     GDCYLKTNQKQKAQMAFASAAKDSFDKQVKEESTFNFAVLTYQLSDKPFNSAIKAMTD
                     YLDAYPNSLRKDEAYNYLILACINTQNYGQALKYLSNVRKNEPKVKKAYQRASFFRGL
                     ELYNNLEFDTAAVLFESSLKYAEQEPILAARANYWLGEISFRKKLYDEALEYYNLFIK
                     SPVAKQASEYSMVSYNQAYCYFNMKNYSAANENFQKFYKTEKPKKSKLYADACSRIGD
                     CIFSTGQYSEAITYYNEAIVADAADKDYATFQKAFAMGLMGKHPQKIEILNNLLKDMP
                     QTKLADNALFEIGRSHVVLQNNPKAVKSFKSIIKDYPSSSFVPKAMLQLGMIDYNDGR
                     NDSAIARYKQVIALYPATPEARNALNGIRTIMIEQDQLDAFMAYAETLGSFANITQSA
                     QDSLLFFSAEKIYTSSRFVEAIDAFQKYIDKFPSGNFIINAWYLQGICYIAMSRQSDA
                     LKNFEKIIAKPRTSFTEPALLEASKIYMSKKNYQEALAYSIILDSIAETPENAMIAKL
                     QKTEAYFVLQQYNECITVASKLLALPSLTPENERKARYFLAKALYMNNEEAKATEQLR
                     RLAKDIKNAEGAEAEYLLIKMLYEQKQPDKAYKEIFKFIDRNTPHQYWLAKAYIILAQ
                     IYAEKNDKFQAVKTLESIIENYDISNDGILDEAKKLKNKLQNNKTQKSSDDEFKIAEP
                     INDLQ"
     gene            19640..21298
                     /locus_tag="PK252_08295"
     CDS             19640..21298
                     /locus_tag="PK252_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14749.1"
                     /translation="MNCNISKNIIFTGIIAFASMSLTGQDLKKDITVTKSYIPTIQEA
                     AKINQMPVFNDTGYVEPDYKYQIKPLAFHLPYQIKPLPSAKMAKETWDNVYNNYALLG
                     FGNPYSPLAEISINSGRTKNYMYGINLYHHSANGKVRLKKDPDYKVNAPFSFTSVNGN
                     GKYILDYASIQGNIGYNHHRLAYYGVDTAFLNSYKEDSNKQFYNNAYFDLLFKSTNLA
                     YDQPDYIAKTSYNFFSDKTGYSENNFSLNATYMHPYKKLKLGGSILYQVSEPSQPADS
                     GSSSIFLFKPTVTKKIQNVNISAGLNIWVDNYQSLSTIYSQPFASLDVVVVPEVLQAQ
                     LGYESQLQLNNATYLITVNPFMKSGITSPTTYSKNIFSANITADISNTATMALAVSYS
                     NNKDQVLWRNDATSTLKNKFVPVIDDVETIKVKTSLIFTPTKSFNATIEAGFNNYTTL
                     REKKAWGLPSFEGNTTIRYNIQDKIISTTQVYLVGPRYYETVANTSKSLPAFVDFNEN
                     IEYQYTKLFSIFVQVKNLTASQYEYWNNYSSYRFQFMAGVRYVF"
     gene            21641..22852
                     /gene="rocD"
                     /locus_tag="PK252_08300"
     CDS             21641..22852
                     /gene="rocD"
                     /locus_tag="PK252_08300"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOT14750.1"
                     /translation="MTTQDYIALEDKYGAHNYHPLPVVLEKGEGPFVWDVEGKKYFDF
                     LSAYSAVNQGHCHPKIIKALKDQAEKLTLTSRAFYSNTLGEYEKFVTSYFGYDKVLPM
                     NSGAEGDETALKLCRKWAYKVKGIKDGQAKIIVCDGNFHGRTITIVSMSTDPDSYGGF
                     GPFTPGFIKIQYNNLNELAKALEDPDVAGFLVEPIQGEAGVYVPDAGYLKKAYEMCKA
                     KNVLFIADEVQTGLCRTGKKLCCDHEGVRPDILILGKALSGGVMPISAVLADDEIMLT
                     IKPGEHGSTFGGNPLACKVAIAALQVLEEEKLAENAERLGKIFRDEISKIQSPLIEIV
                     RGKGLLNAVVIKPHKGKEAWDVCVALKENGLLAKPTHQHIIRFAPPLVINEAQIREAI
                     DIIGKTIKSFE"
     gene            23018..23803
                     /locus_tag="PK252_08305"
     CDS             23018..23803
                     /locus_tag="PK252_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOT14751.1"
                     /translation="MTKIIAVLLFLTTLCVKGQESKYQLIWADEFNQSYLDTNYWTIE
                     VNGHGGGNNELQYYTDRASNIRLENGLLVIEAHQEPFLEKQYTSARIKTQKKAFWKYG
                     RFEARMKLPYGQGMWPAFWMLGENISSVGWPKCGEIDIMEMIGGNANDSTVYSTLHWA
                     NIEDGGHKQHGDTLKLISGKLADDFHVYAVEWDEKTIKAFCDNACFFIIDINSPEMHA
                     FHNNFFIILNYAVGGNWPGSPIETTVFPQKMQVDYVRVYKKHD"
     gene            23914..24807
                     /locus_tag="PK252_08310"
     CDS             23914..24807
                     /locus_tag="PK252_08310"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HOT14752.1"
                     /translation="MLTKKLLYVALSTMVLACCTPEEGEDLVDPNNPPAKEIEYGMEA
                     KIGKTSWKAVNFSIQMIGKNLIISGTSDTMGTITIHLQDTAVGTYYLNPASGNYAFVA
                     DTISDALGYTFGNSLAGGAVIINKVDRDSLTISGNFIFTAYNGVRHTTTTYSQGSFIN
                     IPYSETSLPKKGMISYINNKKVTADSVKAGIAKDEIMFMAYFGNDEIGIDIKNVFKGN
                     YPLGKEYAHLARYNTIGDTILYTTKDNNYVGGIVSITNITKDSLASGSFVFTVYRAKD
                     RKIMNFNRGIFNDIKLNNYTK"
     gene            24808..25803
                     /gene="mgrA"
                     /locus_tag="PK252_08315"
     CDS             24808..25803
                     /gene="mgrA"
                     /locus_tag="PK252_08315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HOT14753.1"
                     /translation="MMAYTANEARYNNMVYRRCGKSGIKLPMISLGLWHNFGGVDVFE
                     NSREILWHAFDNGITHFDLANNYGPPAGSAEETFGQVMDKDFRSYRDEMIISTKAGYY
                     MWPGPYGEWGSRKYLVSSLDQSLKRMKLDYVDIFYSHRPDPETPIEETMMALDHVVRQ
                     GKALYAGISNYTAEQTQKAAEILKSLGTPCLIHQARYSMFDRWVENALLDELENQGAG
                     LIAFSPLAQGLLTDKYLHGIPEGSRASKEHGFLKPQHITDEKLDKIKQLNQIALDRGQ
                     SLAQMAIAWLLKDNRVTSILVGVSKKEQLYDCIKSIEKLEFAKDELQKIEKILGV"
     gene            complement(25800..27068)
                     /locus_tag="PK252_08320"
     CDS             complement(25800..27068)
                     /locus_tag="PK252_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT14754.1"
                     /translation="MELLSSSWFLVILCLLVSAFFSGMEIAFVSANRLRIELDRKSGI
                     FTSGIVQIFVKNPSQYIAVVLLANNLVLVIYGLIMAKMLSAPLMQVLNNEVSVFVLQT
                     IISTLLIITAGEFFPKFIFRLNPNFFLNYLSVPLFVFYLILYIPAKLIMMFSEWILKL
                     VGKNSDTKNTKEVFSKLDISYLLEQTEDKQIQDIDTEHEIRLFRNALDFSSVKVRDCM
                     IPRTELVAVDIKTSIDNVKNKFIESGFSKLLVYKENIDNIIGYINIKDFIKNPNNIKT
                     RIIPAPFVPETMNANNLLHSLLQERKSLAVVVDEFGGTSGIVTIEDIMEEIFGEIEDE
                     HDKNDIVEKQLSEDSYIFSGKIDIETLNSKFQLNIQEDENYNTLAGFVVYKLDSIPAQ
                     NQVFEIDHFQVKILKTVNKRLELIYLKILN"
     gene            27429..28415
                     /locus_tag="PK252_08325"
     CDS             27429..28415
                     /locus_tag="PK252_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOT14755.1"
                     /translation="MKASYKVSIIIAGLVLLSFLGYSVVCGSSTKKVNDQYIISAAEL
                     PEALAFAGDSVPLQKFDVRESLDREIHINIYFQSQTILILKRANRFLPEIERILKEEG
                     VPADFKYLAVAESGLTNVVSPAKAAGFWQFLKSTGQKYNLKITDEVDERYHLEKATRA
                     ACQYLKELYGMFGNWTLAAAAYNVGENRLKDILANQKVSSYYDLWLNEETARYIFRIL
                     ALKLIIENPVAYGFSIPEKDLYQPLQYIELSVDSTVTDLARFALAQKSNLKMLVYFNP
                     WIRSNSLTCKTNEKYTIRIPAPGFRESVESSNYQSYLQQNCDTTSKHKTTSF"
     gene            28478..31288
                     /gene="uvrA"
                     /locus_tag="PK252_08330"
     CDS             28478..31288
                     /gene="uvrA"
                     /locus_tag="PK252_08330"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT14756.1"
                     /translation="MEDPDNIHVYGARVHNLKNIDLSIPRNSLVVITGLSGSGKSSLA
                     FDTIYAEGQRRYMETLSAYARQFVGNMERPDVDKITGLSPVIAIEQKTTNKNPRSTVG
                     TITEIYDFLRLLYARASIAYSYNTGEAMVRYSDDQIIKIINEKYKGKKIYLLAPIVRG
                     RKGHYKELFEQIRKKGFIQVRIDGKIEELRYNMRLDRYKTHYIEIVVDKLIAGEENSK
                     RIKDSLAVAMRQGKGIIMVLEKDEEKVQYFSRLLMCPSTGISYNEPAPHSFSFNSPQG
                     ACHKCNGLGQIAEPDMNRLIPNPKLSIKKGGIAPLGTYKSSYIFLIIEALGKKYDFTL
                     DTPLENISEDALNTILYGTDEPLQVKSAYGGLSNYTLSFDGLINYLNQNAEKEDDTKK
                     SELNFFKNVVCPECNGTRLNREALHFKIDGKNIAELSAMDIADLAKWLEGVEDRLSEK
                     QQAIAREILKEVRTRLGFLLNVGLDYLTLDRSSRTLSGGESQRIRLATQIGSQLVNVL
                     YILDEPSIGLHPRDNKRLIKSLQNLRDANNSVIVVEHDREMIESADYVIDMGPKAGRH
                     GGDVCAHGKPEDLKNYCSITSLYLTNKRKIDVPDKRRKGNGKTITLIGATGNNLKNVD
                     IEIPLGMMVCITGVSGSGKSSLINQTLQPILSQHFYRSLTDPLPYKEIRGLENIDKVI
                     EVDQSPLGRTPRSNPATYTGVFTDIRKLFELTPDAKIRGFGPGRFSFNVKGGRCEACK
                     GAGIQIVEMNFLPDISVVCKECNGKRYNRETLEVRYKGETISDILDMTIDRAVDFFAN
                     IPAIHRKLDTLKQVGLGYITLGQQATTLSGGESQRVKLAAELARKDTGKTLYILDEPT
                     TGLHFEDIRILIDVLNKLVEKGNSVVIIEHNTDIMKVADYIIDLGPDGGKRGGNIVNA
                     GTPEFIVKSAKGFTAQYLKNEL"
     gene            complement(31407..32069)
                     /locus_tag="PK252_08335"
     CDS             complement(31407..32069)
                     /locus_tag="PK252_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOT14757.1"
                     /translation="MRPAQNQPFRFVRSIKILAFFATIGIVILVTSYFYISLKSNPYI
                     YTDTNKLPNTKVALLLGTSNRLKEGSPNPFFYNRIGAAVELYRKGKVKYFILSGDNQY
                     MSYNEPIKMRNVLVRMGIPDSVLYLDFAGIRTFDSVVRCRTIFGQTEVLIISQNFHLQ
                     RAIYIARYMGMNAIGYRATGVGIIRGLKTLLREFPARLLVYWDLYNQTMPKYLGEKVN
                     IP"
     gene            complement(32140..32469)
                     /locus_tag="PK252_08340"
     CDS             complement(32140..32469)
                     /locus_tag="PK252_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOT14758.1"
                     /translation="MKKIILLIVVVLSFSGFMKAQSSDTLKIQTSAQCEMCKERIETA
                     LAYEKGVVSSDLEMNTKILTVVYKPAKTNADKIKAAISWLGYDADDVKAGLKAYNKLP
                     ACCKKKE"
     gene            complement(32524..34740)
                     /locus_tag="PK252_08345"
     CDS             complement(32524..34740)
                     /locus_tag="PK252_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14759.1"
                     /translation="MNKIFILIVSLCLTFAMAYPSSVLRGKVYYINEQGDSVALPYAT
                     VFWENTTINTQTKEDGSFSINKPHGKTNIIASYVGYKADTIATKPEEEFISIVLSANS
                     QIEEVTITKKTEGNYISVLKPMKTEVISQAGLHRLACCNLSESFQSNASVDVGFADAV
                     TGARQIQMLGLSGIYSQLMFENIPLMRGLSIVNGMSYVPGSWMEAIQISKGTSSILQG
                     YESITGQINIEYKKPEEEPPLFINVYAANDSRMELNANGVIVSGHDWNGMLMAHVSGN
                     FNKMDENGDSFIEQPLGRQINIFNRWKYTSEHVEQQFGFRFLNDEKQGGQMDFNQRSD
                     LDKDIYGFESKIKNFQLYSKTGIVVDSTEYNGFGLISSYTYYDNNSFMGYNDYKGRQH
                     SIYANLIYQTMISNSSHKVSTGISTNYDNYNQLLNDSAFKKQEFVPGLFAQYTWSPSD
                     KFTAMAGSRYDYNSRYGWYLIPRGHIKYELFGGIVLRASVGRGMHSAEVIAENLNYLV
                     SARKYIFTEQLNLEKAWNYGFNITKTFAFVEGRSIQWSADYYHTTFDNQVVVDIDANP
                     QSIIVYNLKGKSYSDAFQTDLNIHPLKGLEINTAYRINDVKQTVHGELKSKPFVVTHR
                     GLISASYAFNHRKWQIDATTQFNGRAPLPNTSPNPAEYQLAQYSPAFINVLAQVTRRF
                     KHFDVYIGGENLTNYTQKNPVIAANAPFSNYFDASMIWGPVMGRMFYAGIRYTLKD"
     gene            35410..36912
                     /locus_tag="PK252_08350"
     CDS             35410..36912
                     /locus_tag="PK252_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14760.1"
                     /translation="MRQFLLVGISQFLIIASFAQKPVWVDFEKRKQLYPNDKYIVGFY
                     SAPKKTDKPDDSQLQTLLEYAKTDIIENIYVSVTSASELNTRQTGKEFIQSFSQQSLM
                     ATKAELAGLKTETWKDKKELYALAYVSRTELISFYKGKLATMLANIDKSVTAAELEKT
                     NGDKEKALDMFYQSAKQVRDAEQAAAILLGLMKQVPANNLADLNTRINNGINAIISRE
                     NPTPDAAALTIAGMLKKQISATAPKVKIASPTFQDTKMGSPFSAYFSKMLESKLGSRG
                     FNIEQNTQNNSDYVLNCTYWKEADKLKLMLNMYAVKTGKITATAEVSVPQSWFAQNNI
                     AFMPENFEESYSRAKAFTKDEIVGGGLRIDLWTNKCDNNPVFFENDTLKIYMRVNHEC
                     YLRFVYYMADGSKVLLMNNYYIASDKVNKVIEIPESFVCAEPFGNEILVVNAQTEPFQ
                     KLNTRDQDGYTFINENVTEVVAKTRGFTKTTNQTLNGEKRLNLTTLKKQY"
     repeat_region   37164..37980
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=37164..37199
                     /rpt_unit_seq="atctacaatagtagaaatttattaggctttatagcc"
BASE COUNT        11836 a         7635 c         6782 g        11756 t
ORIGIN      
        1 aatatttacg tactatcagc aaaccacatt aacccccaac tcctgtagct gttgtacgtg
       61 taacgcattg acagggttgt ccaacacttc caccagttca agctcagtaa gttcaaataa
      121 aggtgaaagg tcgcttatat tgttgttatt gagaaatagg cttttcaaat ttgataaatt
      181 gctcaatgcg gcaatgtcat gaatctcgtt atcagaaaga ttaagttctt caatattttg
      241 aagagagcaa agtaaatcaa tatcggcaat cgaattggca gacaaatcaa gctttacaac
      301 atgcttgcag tactggactc cttcaaggtc gttaatatta gaagccgaca actcaaattc
      361 atcaatatct tcgagataat acgttgggta ttcaatctca gtatgcccgt aatacctgat
      421 tttgatagca tcgtaaaacg tcaacccatc atttttcgta taattttgtt gttgaaaagt
      481 cgtctttttc tcgtaatcat taacaatacg aaagccctca gcctcagtat cccaaccata
      541 cacagggctg taatctatat cctcaaagct atcgagcgat tgggctattt cttctatctt
      601 ttcaaggcgc aggatatgcg aataatccaa caacctgtca aaatcattag cattggcaag
      661 cttttcaatt tctgaaatat gaaaagccgc tttatccggg tgatacaaca tcatcagctt
      721 tgaaaagcct tttcctttgt cgtctatctc aatgtctatg taatcaccta tcatttcggc
      781 aatcttttga agtaccgagt attgttggct ttgataaaga ttaataagca caagcgctat
      841 cttattcaga ttgtcaacag cataagcatt tacaagccta tcttttagtg ttttaacatc
      901 catatttatt ctattttttt aaacaaaaaa atcccgggac catgacagcc ccggaactca
      961 acgaaccaac acataaacta attaactaca aaccttcaac ttatctgact attattgtta
     1021 ccggtatttg ataatgcatg cggacagcaa agccattcat tttgaccgct tcaaacacgg
     1081 gagccttact tattatgtct gctaacattt tatctatttt gggttccacc ttattattga
     1141 gctcataacg cagaattttc cctgttttgc tgacccacaa atcaatatcg caatagtatt
     1201 tgccagcagt gatattgtat tgatctaaat gcatttcagc ctcttttcca atgtactgac
     1261 caataattgc acctatttca gccaattgtt tatttacttt taattcttct ttgccattga
     1321 caatctcact gttgcttacc ttcaaatcac tgtatgccaa tagctcttga gctttaacat
     1381 tgtacattgc ccaaatcact gttatagaaa atgctacaat tactttcttg acgacactta
     1441 cactcacttt ttccatggtt taaaattttt atttttaaat gatggaacaa aggtagtttt
     1501 gattgtaaag cattgacaac tattatatta cgaacgccag ataagaagaa ttgaatgcct
     1561 gtttttatat tttaaagaac tttataaagt atcattgttg tccggtaaaa acaggtcgcc
     1621 gctatgcggc ttagaaacat ttggtgtatt tggttatcct acaaacagtt cgcagctacg
     1681 ctgctattat ttagaggttg tgtaaaaagg tcaaatttgt tcatttttcg actccgctca
     1741 aaatgacaaa ttgctgatta tcaatcgtca ggctgagcga agtcgaagcc ttttcagact
     1801 ttttacacac cctcatcctg tttgtagtat aattaaacat cgttaaaatt tagccccagc
     1861 ggggcgacca atttctaatc aacgatatta ccagtattta taagtatcta ttttatttta
     1921 ccggacaaca gtgataaagt attagaaatt aaaaagctct gcggttgcta atgttatttc
     1981 tctccgtcaa gccaccgctt aaattcagcc actttatcaa ggcttacata caagtcctcc
     2041 tgacaaggcg gattgaggtc tattttcaac ctgcttttgg gaaatacatg cacattggca
     2101 atggctttat gatttataag gtattgacgg ttgaccctga aaaaatcctt tggattaagc
     2161 tcattgtaaa gtttgtcgag gctcaagtct aatgaatact ggtttttgct gttcatcacc
     2221 acgaaagtaa tgccactggt ggaaaagaag taggcaatat ccttcacctc aatagtcttg
     2281 agcttctccc ctactgatac cgagtatctt tcgcgatatt ccttgttgtt gccttgcata
     2341 agtttataca gccctgtcaa atcaagctgg gcagattgag taccagtcca ctccctgtat
     2401 ttggcaatgg ctttgcccaa atcgtcctgc acaataggct tgagcagata gtctatgctt
     2461 ttgtgcttaa aagctttgat ggcatattcg tcaaaagcag tagtgaaaat tataggcaca
     2521 ttgactttta cctgatcaaa aatctcaaag ctgatgccat cttccaaatg gatgtcaagg
     2581 aaaatgaggt caggatggct gtttgtttta agccatgcta cagaatcttc gacagattcg
     2641 agctttgcca ttacttcaat ctggggatca atagttttaa tcatcgattc caaacgacga
     2701 gcggctaatt cttcgtcttc aataatgatt gttttcattg gttattaggt ttaaaaagat
     2761 tacttggtat taaataaatc gttgaaatat tgggcatcaa taattctctg cggaaaatcg
     2821 cctgacgaat atttgtccag cattttgtta agttcatcct ggacaatagg tttgtgcaaa
     2881 tagtcaatcc ctttagtgac aaaggcttta acggccaact catctgtagc tgtagtaaac
     2941 acaatagggc atgttacggt tactttatta aaaatggcaa aactaaggtc gtcttcaagg
     3001 tgtatatcca gaaaaataag gtcaggatgg gtattttggc taaaccattc gatggaatcc
     3061 tgtactgact caagccaggc aacaatttga aattgtggat tatagtcttt aaccaggttt
     3121 tccaacctgc gtgctgcaaa tgcttcatct tcaatgataa caattttcat ttttttgtat
     3181 tttgtaactt attatttaaa actatccgat tttcaagggt tataatatct gttccgtttt
     3241 tttcaatggt attttggcta taaaatgggt ttctgttttt tcaaactgag gagctggtaa
     3301 ttccaacagt tcatagcgat gggcaatatt tttcaatcct acacctgtag aggccatgtg
     3361 cgattcacgt tcctgcaagt tatttataat attgagcatc tgttgcgcat cagcaaaaat
     3421 ttgtatcacc agcggggctt tcttggacat ggtattgtgc tttatagcat tttcgataat
     3481 cagctgaagt gccaatggca atatcaggta tgctttgcag tccatctcta tgtttatttc
     3541 caccttgatt ttgtccataa acctgatgtt gagcaaaaac aggtaagcat ctaaaaagtc
     3601 aagctctgtc tgcaaactaa ccaggtcgtt gtctttattt tcaagcacat agcgatatac
     3661 ctttgagaga tgctcggtaa acttctctgc caggtcaggt tcgagcttga taagcgaagt
     3721 tagcacgttc agactgttga acaggaagtg cgggttcacc tgctggcgta aaacctcaaa
     3781 ctgcgattgc aggttctcct tttgaagcct gataagctgg gtattggcct cggtaagctt
     3841 tttaaccgcc tcatttacct gtatttcaag gttttggttc agctcctgca acttttcttt
     3901 tgtgatccgc agctcttcct catttctcct gcgttctgta atatctgtac ccacgctaaa
     3961 catatccgtt acttccccgt ccaaattctt tattgcagtg ttattccacg aaatccatac
     4021 cttttcacca ttcttacaag tattttcatt catgttgtgg gcatattttt cgggcgtttt
     4081 aaatacatct tccatcaact gcttcaaatc ccttccggta tattgttctg atggcgatac
     4141 tattgtccca acaataggtt tcccaatgat ttcttctttt tgatacccaa aaaatttaag
     4201 cccgtattca ttcaaaaatg taatttcaaa tttgttgttc catttgagga tgatgctatt
     4261 ggtggtttcg accaattcgc ggtatttttc ttcgctttcg gcaagggcct tttgattttg
     4321 ctcacgctca gttatatctc ttgaagttac aatctggatg ccttttccgt taagtacggt
     4381 aaaacctgca cttaattcaa caggaataac agacccgtct tttctcttgt gatagcgtaa
     4441 aggcactaaa gtttctttgt tatttgtagc tgttttggtc ctttcgggct cagcagagac
     4501 atcggtattt ttcattttca aaaattcctc tttgctatac ccgtaaatac gagtcgcagc
     4561 agggttggta tccagaatat cgcctgtttc aacatctatc atgaatatag catcagattc
     4621 ggcttcaaac agcagcctat acttttcttc attttcctga atggccaatt gcaatcgttt
     4681 actttcggta atatcatgcg aagtgaaaaa actggcccat tcaccctggt attggatcaa
     4741 ggttgatgtt gtttcgcatt ctcttatctg gccatctttt gttaaaaaaa ggaatatttc
     4801 attaatagtc gaaaaatcaa gctcatttct tctcttggca atggcaaaag ctttttcatg
     4861 ctcgcttggg tgcaaagtgg ccactgatgg catattgtac aattcttccc ttgtatagcc
     4921 aagcattttg caataagtat cattgacaaa taaaaattta ttgtcccgga tgatagtaat
     4981 gcaatcctgc gaattttcaa ccaacgtctt atatttagcc tcgctttcat gaatggcttg
     5041 ctccatcttc aacctctcgg taatatcgcg cgtaataacc agaaatgcag gcctattctt
     5101 ataatcgata accgtaacat gaagctcgac agattttttc tttccagatt tggctatagc
     5161 attccacctg gtatatctgg taaacttctc cccgctttca atcctgcgga acatatcgtt
     5221 catatcgtcc atatcttcgg cgctgactgc ctgcataacc ggcataccca atacttcatc
     5281 catagaatat tcaaggatat cacaaaaagc tttgttggca aaaatcaaat ttcggtcctg
     5341 agttatagtg attccgtcta tggcctgttc tacaacattt ctatactgta gttcgctttc
     5401 gcgtaaagct tgttgcaatt tttcacgttc ggtgatatcc cgcaatgtaa ctaaagaaga
     5461 gtttttgcct tttataacta cggtgcttga gttcatttcg gctataaaac ggctgccatc
     5521 tttacgaaca aatgttgcag tataggtttg cgctttaacc tcgccattta ttcgtttagc
     5581 gtgatactcc attacccgtt ctttgtcttc tggagcaatg atgctggttg gctcaatatt
     5641 cattaattct tcatgggtat aacccatgag gttcaggaaa acgtcattta cataaataaa
     5701 tttataatcc tgtgtcagca caacgctatc aagagtattc tcgataagtg aacggtaaag
     5761 tgcttcactt tcaatcaaat caagctccat ctgcttttgg tcagtcacat cttgctgaat
     5821 aacaatatac ccgtcaacat ttccagcttc gtcgttatat ggcgaggcaa tggtgtttgc
     5881 ccaatacatg cggccggtct tatgcctcga gtatagttcg ccctgccacg attcacccct
     5941 taacagcgcc tcagctatga atttccttga tgctggaatt tctccgttat aaatggtttc
     6001 atttatatgc ttatgcagca aatcttcaac cgtataacca gattgtttgg caaattcagc
     6061 attgacatat tcaaaatgca tgttattatc aataatataa gcggcaacag gaacctgatc
     6121 aagcaccaac ctgaacttct ttaggttata ttctgccatt ttctgctctg taacatccat
     6181 tgccactcca atatatccat atttcaggcc attagtattg tacaacggcg agaccctggt
     6241 atcataaagc aaatttttaa cctttacaat atcttgttca atggttccgt ccaatacttt
     6301 tctaacaaat atttcagctt cagtgccttt ataaacatca aaaacagatt tgccaatatt
     6361 atctcctgct ctaaccccaa tattttcaag gcctttgcct tgagaataaa taaatctgcc
     6421 ttctaaatca atgctccaaa ttaccatcgg ggcattttcc aatatactat ccagcaattg
     6481 ttgtttttct tgcaacttgc atgtcgtttc gtgatggctc tctacttcag ccctcaattc
     6541 ttctatcgca gcctgaagct cctcattggc ttcctgaatc ctaatactgg aaagaaaatt
     6601 ttcagcatcg gctttgttcc tgttaatagc tgcaatccag ccaaccatga ggataccgga
     6661 ataaatggaa aatatcctga aataaacctg ggcatcaata aaatctttgt gttgggtaaa
     6721 gataacccct gtaaaaattg ccacaaaagc caagccattg aatgtgagtg ctgtataaga
     6781 aaatggcaat aaaataaacc atccaatcaa aacaaaaaac aacccatacc aatatgggct
     6841 ttggcctgcc ccggtcaata atgccaacaa cgtacaaaac aaagtgccaa tataaaagcc
     6901 aaagatgata tgttgcttgt atgctttatc attggccctg attagaccaa ctaaaagatt
     6961 taacagcata gctgccaaag tcaaccccct gagctgaaac agtaccttcg actggtgaat
     7021 accatacata ccatcgaaaa actgccataa ggcgacaatg attgtcccgc ccaatgtcag
     7081 ggccagtaaa agttgcctgg gagatttcag ccgaaagtct ttaaaacttt cttttttgtt
     7141 tagttcgttt agctccttga ggttcataat caagatttta aaaagccaag gtacttgaaa
     7201 tccttatttt ctttaacatt ttttctttat tttatatctc acatgaaatc gtttagttta
     7261 cgggtataat aatcgctaaa aatcttcttt gacagttctt ctgcccattt ctcaacaccc
     7321 atttttttta tcaggcataa attatacact ttcaaggcat gaggtaccct ttcggccctg
     7381 tcagcaacag gttgtaacct gaagcatgga aactgcccgc attcaaaaca aaactccacc
     7441 ttatgttcct gcacacattg gtatgtctta caatgagggt ctataccaag aatctcgaga
     7501 cccgggcaat atcctttatt gtcgcgacag ccaagacatc ggtatttttc gtcaggaata
     7561 ttgccccttt caaaaaattc ccttctgttt tctttgtttt cttcgcctat atacaaagga
     7621 cagttaaaac aatccttgcc acatggggct gtcatatttc tgtaatccat ttttcgcatg
     7681 ttttaatagt ttaaaggtat tttttaagaa acatttcttt ctttcaaaaa aagttgaatg
     7741 acggaaattg aaccatgaat gacgtatgtc acattttaaa atcaaaatac gcagtaagaa
     7801 aatctcttat ctgcgtattt tattacaacc aatcaactaa ctatgtaact aactaattta
     7861 tatctaagca attaaattcg taatctgtat tttttaattc ttaattgtta atttttaatt
     7921 gtttaaaaag cccctcctcc tttgaattcc aaagcatcgg ccctgccacg gtttttgtat
     7981 tggaagttgt tgaaagagta tgaaaagttg agcataaacg ttgtaccttc tgcatttatc
     8041 ccgtaaacat tgtcaaagcc tgctcctctt actttataat caaactttat tgaattgaaa
     8101 atattttggg ctgaaagact gatatttccg cggtctttga gtatagattg gcttatagcc
     8161 atattcacag tataaaaacc attaacatca cctacagcat caatacttgg tgccctgtag
     8221 taaaatactg tctgaatgcg ggttttccat ttggtactga aattattgac atagcgggca
     8281 tcccaggtat ttacttcttt tttgttttcc gaagccccgt tcatagtttt taaaatatag
     8341 tgatatacat tagcaccact gtttatttgc caccatttgc tgaggttaat atctattccc
     8401 tgctcaacac cgtatgattg ttggttgtcc gaattgatca acctgtgata catctttccg
     8461 tctgtgttca aatatcttga agtctggaaa cagttggtgg tatttctgaa ataagtctga
     8521 gtggagaatg taaatatttt aagggttgta cgataattga attcataagc atcagtatat
     8581 tcaggcttta gataagggct tccaatgaaa acgttcttcg aatcgagata tgagggatta
     8641 ttgctcaata tccaaggctg aggcctgtta atacggcggc tgtagctcaa ctgaaactgg
     8701 tgcttttcat ttatgctctt tgacaagtgt accgatggat aaaacatgaa tttgttgtac
     8761 tcaaaaggat cggcatcggt agatatctct atttttctca aaaatacttc acctcttaag
     8821 ccaagctgat aattgatgcc ccatacagag ccagaatatg ttgtataacc tgaatatatg
     8881 gtattattgt acttaaggtt attagaaaac gtatcattct tgacccataa ggcttggttc
     8941 acatcatagt tttcaaaata caggtcttcc caacgatact cataacggaa ctgagctcct
     9001 gcctcaaaat taccagataa taaaggcgaa gaaaaatcta catttgcacg atagttataa
     9061 ttcttattgt ctttatgatt gtgcagcata gatgaaacat caatatattc gtaaacacta
     9121 ttggtattaa gttcattcag tttattatca tcttcgccct tccagttgta aaagatatta
     9181 gtaaatgata ttttactatc tttagtgaat gaatggctgt agtcggcatt caggctatat
     9241 acatcaccca taacatccat gtagttatta cttgaaaaat aagttgtatt atcaacaata
     9301 tcaggtatta cacgagcctg cgaattgctc ccgttgtcat atccttcacg gccaatggta
     9361 cccgaaaaag ataaagaatt catagtatcc aggtcataat caattccagc tttaaaagac
     9421 agtttgttgt tatagttgaa ctggtctgta ctctgcaaag tatgtaatga agcgttgttt
     9481 tgggttatcc tgctattaaa atcaatcact tcggagtact tattgtcaat aaaatcaaca
     9541 cctccaaaaa agtttgcctt tccagcccta taatttaaaa gcacattgcc atttctcttg
     9601 tcgtgtgtcc cggcagaagc gcttacatta ccattcaaac cctgcaattt gtctttcttt
     9661 agtataacat tgataatgcc cacctgcccc tcggcatcat acttggccga cgggttggtt
     9721 atcacctcta tttgcttaac agcactggca ggtatttgtt tcaaagcatc acttcctttg
     9781 acaacagatg gtttaccatc aatcaacact ataaactcag aactaccgcg caaagtaaca
     9841 ttgccctgtg ggtcaacctg tacagatggg gtattttcca aggcattagc tgctgtacct
     9901 cctttagcat taaggtcttt gtccacattg ataacacgtt tgtcaatctt atactcaatc
     9961 ctcgattttt caccttttac aaccacttca tctatcttgg tattttttga tttcatggct
    10021 atatcgccaa gattggcgct ctttcctgct tttgaaacac taaaagcatc tgaacgatat
    10081 tcatcataac caataaaagt agctttaacg tagtaactcc ctattttcag gtctttaatc
    10141 ttgaagtttc cgttttcatc ggtcattgtg cccgagacca agctcgaatc agaggtattg
    10201 aacacagcaa cactggcaaa ttcaatagga ctacctgatt gttcatcaac tattttgcca
    10261 tttactgaat tgaatggcat ttcggccatt ggctcagcgt taacacctat tgcaaaagct
    10321 attgccatac aactggtgat ggtaaataaa cgaagtaaat tgtttgtttt catttgaata
    10381 agtatttaat tgttcttttt gatttctaat taccatgcaa aattggttca gcaattgtta
    10441 acagatgtct ccacaatttc atggattgta aaccattgta aaaaattgta aacgcacagc
    10501 taactcactg atttgcaagc aaagttgcat acatcaaata cttaacttca aaaatgactt
    10561 attgaatgac gagtttggag gaatgaaaac aaaaataaaa ccctccggca gcattatact
    10621 cccgaagggt tcaataaaag ggctaacccc tcccttaata ttaaaaaaaa tgtcaaaatc
    10681 tgtttagaat ggcaaatcat ctaccggagc tccaactccc ataggaggtt cttctgtaat
    10741 aggtgcagct tgccaatcag aaggttcaga ttgcgacccc cctgctattt caatcttcca
    10801 tgctttacaa tcggtgtacc acttgccatt gaattcacgg ctttcaacat caaaatcaat
    10861 tttaagtaaa ttgcccactt gaagctggct ctcattaatt ttatcacccc agatagcaat
    10921 acaaaccttt ttaggatatt gcccatctgt ttctacaata atatcttgct ttctccattg
    10981 tccgtttttt ccatttcctg tttgtagtgg caatacatta attaatctgg ctgtaagttg
    11041 catctatctt tagttttaaa atttatacaa aaggtgattc tatatcaaaa ttattattga
    11101 ccggcaatga attcatatct actccagaca aatcatcgtt catttttgag ccaacagttt
    11161 tgattggttt tacttctata ttatcatcgt acggcaacac atcttcaaga tctataaacc
    11221 gggctatgaa gtcacggaaa cgcaagcgta catcgccaat ggctccatta cggtgttttg
    11281 ccacaataat ttcggctata ccaatggtag ggccatactc gtcttctgta ataccgtact
    11341 tttcaggacg gtggataaag cacaccaagt ctgcatcctg ctcaattgca cctgactcac
    11401 gaagatcaga taactgaggg cgcttattgc cacttcgcat ttcaactgaa cggttcaact
    11461 gcgacaaagc tatcaccgga acattcaact ccttggccag cgccttgagc gaccttgaaa
    11521 taacactaac ctcttgctca cggttggcac gggtttctgg cgggccagtc atcagctgca
    11581 agtagtctat gataacaatg tcaatatcat ggagcttttt caaacgacgg cactttgccc
    11641 ttagctcata aatagagata ccgggcgtat catcaataaa aatcttagac ttttccaatg
    11701 gttttacttt ggcttccaac tgttgccact cctctgtaga aagctttccg gtacggatac
    11761 tcgacgaggg caattctgtc tcgcacgaaa taagacggtt taccaactgc aacgacgaca
    11821 tttccaacga aaatatggct actgattttt gataatctac tgccatattg cgggtcatag
    11881 aaagtacaaa ggcagttttt cccatcgatg gacgtgcggc cacgatgatt aaatctgaac
    11941 gctgccaacc agcggtcatg gcatccaact tggtaaaacc agatggcacg ccacttaaac
    12001 tgttagtatt tttaccagcc tcttcaatct ggtgcaatgc ctctgaaata agactactca
    12061 cttctactgc atctttctta atgttgccct gtgccacatc aaacatttct ttttcagcaa
    12121 agtcaagcaa atcgtccaca tcaatagaat cgtcgtacga acgtgtctgt atttctgagc
    12181 tgatacgtat caactctcgc tgaataaatt tttgctggat aattcgggca tggtactcaa
    12241 tatttgcagc agaagctaca cgttctgtaa ggcttgacac ttcgtaagcc ccacctatct
    12301 catccagatg cttttctttt tttagttctt cagtaacagt aagtatatca ataggctttt
    12361 ccttacggga aagctccatt gctgctttat aaatcttctg gtgtttgtct aaatagaaac
    12421 tttcaggcct caatatatcc atcacggcaa taatggcatc acgctcaagc atgatagccc
    12481 ctaagacagc ttcttcaatc tcgattgcct gtggtggcat ttttccaagc tccgacaccg
    12541 gaactggtgt actgatactc tgcctgttag aatattgttt ggccataaaa actttttgtc
    12601 caaatttata cattctcggc aatcagcaag acgcttttaa aaattgattt gataaaaaat
    12661 aatgaacagg ttatatagtt gaatatcaat ttttaaaatt aaaataaaac ctattccttt
    12721 actctttcct acaaggaaga ggtgttaaag ctactactaa attaagcgct attcttcaat
    12781 tatgcggaaa cgttgaaagt gtataaaaaa agcccttgaa aactttcatt tccaagggct
    12841 tctctaaaac actttaagct tttatgctat ttccaccatt aaatggtttt tagcaatttt
    12901 ttcgtcaatc tttacattaa ttgactttac cgtcccatca accggagtcc tgataatatt
    12961 taacattttc atagactcaa caataaggac tggttcgcct ttttttacaa cctgtcctac
    13021 attgaccttt atttcgcgga caatacccgg gataaatgat gttatcaatt ttgggtttac
    13081 aggttcatat aactttctgt tcctaaattt ccgggtaaaa gtagttatat aatcagcatc
    13141 atcaagatgc aatttttcaa tattttcagc cataacattt cctcctatca aaatggtggg
    13201 ataccatgtt ttttcttagg catgctgata tccttgcgtt ctgaaacttc aatggcatgg
    13261 ataatgtaac ggcgggtttc gccaggctct attacagaat caatataacc tttagaagca
    13321 gcaacaaacg gattagcaaa tttttctgaa tattctttta ctttctcttt acgcattgct
    13381 tccgggtcag cagcttcagt aatttcttta cggaaaataa tgttagctgc tccttctggg
    13441 cccataacgg caatctcggc acccggccat gcaaacacaa agtctgcacg taagtgacgt
    13501 gaactcatgg caatgtaacc tcctccataa gctttacgca agataacggt tgttttaggt
    13561 acagtagctt cgctgtatgc ataaaggatt tttgccccat gacggataac acctgcatgt
    13621 tcctggtcaa tacctggcaa ataccccgga aggtcaacca atgtaactaa aggaatattg
    13681 aatgcatcgc aatatctgat aaagcgggca atcttatctg atgaatccac gtcaagtaca
    13741 cctgccataa caaggggctg gttggctaca aaacccacag tttcgccatt aacacggcca
    13801 aaaccaatta cagcattagc tgcccacatt tcctgaactt caaaaaaatc ggaatcgtca
    13861 acaatagcgc ggataacgtt acgaacgtca taaggctgtt ttgggtcttc aggaacaatg
    13921 gtatcaattt tgtactcttt tttaggaggt tttggagctg tttttggggc tttttcactg
    13981 ttattccaag gaataaatga aaggagcttt ttgatttgct caaaacattc gtactctgtt
    14041 tttgcaaaga agtgtgcatt accagtaatt tcagcatgca cacgggcccc accaaggtct
    14101 tcctggctga tttcttcacc taacacactc ttgattactt ctggcccggt aataaacatt
    14161 tttgaaatgt tgtcaaccac aaaaacccag tcggtaagcg ccggagaata tactgcacca
    14221 cctgcacaag gcccaaggat aacagaaatt tgaggaataa caccagaagc taaggtgttg
    14281 cggaagaaaa tttcgccata accagccaat gagttaaccc cttcttgaat acgtgcacct
    14341 ccagagtcgt tgataccaat aagcggcata cgcattttca acgcatggtc catgattttg
    14401 gtaatttttc cggcatgcat acggccaagc gaaccaccaa caactgtaaa atcctgggca
    14461 aaaatactga taggattgcc gttgagcgta ccggtaccgg taatgacgcc atctccgtgc
    14521 agttctttct tttccatgcc aaagtcgcgt gcatcatgtt gcacaaacaa atcgtattcg
    14581 tgaaatgatt ctttatcaag caaactatta atgcgatccc tcgcagtgag cttacccatg
    14641 gcaacttgtt tctcaatggc tttgtctccg ccaccttttg aaacaatttc ccgtttttct
    14701 ttcagttcac ggattttctt ttctaatgcc ataatatttt tatttgatta ataaatatga
    14761 ttagagctgt ttaaaatttg aaagtgcaac attacgaaat gaattttaac taaaaaaata
    14821 aattcaggtg aaaataacta acgtttaatt aaccgtctgc accaacaata acaaacacaa
    14881 tgcttttatc ataaacaacc cataaacata ttataaataa aagtattaag cattagattt
    14941 atttagtaac acgttactgc tgcttcgttt gaaaaatccg attcttctgt tgcagatttt
    15001 gcagttattt tgaaataata cgtaccagca tcccaatcta cataataacc gcgcctgact
    15061 atccctgaat ttgtgttggc cggaaaataa tccgggataa gcaccagatt ggttattgac
    15121 gatagcggat aataatttcc atcatcagct ttaatatata cattaaattg tatttcatta
    15181 tctgaattat ctgtccaaga taacgaaata aaactatgta attcgtacat gccagtcctt
    15241 gaattccaac tttctaccct gcgcgcataa cttccgctca gattgcccgg ggccgcaggt
    15301 ttctttgccc cgccttcaat aatagtacct cccgactggt aaaccttgta atacaccagt
    15361 tctatttccc ttttgtatgt aaatggagga taatctgaac cgtcggaggt aaaagttacg
    15421 aactgcgtaa accctacttt atcgctgtaa tactcttttg tatattctgt aaacaaatat
    15481 ctatcggcat ttgacgaatt atactcggac gaaaccaaat accctgaaga tgtcccttca
    15541 ggataaatta cataagcact gctaatggtg gtatttacac tgccatataa agatgatggc
    15601 tttgctgctt ttgtcccgaa aataaaattt atatctttac tattgtcggt caaattgcgc
    15661 catgtaaccc catcgttaga aaactccaaa gccccactgg ttgctttcct gaaataaaaa
    15721 accgaattca atggagtcac tgttgtggca gggtctaaag tacatgaaat agttgccact
    15781 tttgatgatt cattgtaatt cgtaactttc catgtcattt tagccgtttt tacggtgctt
    15841 gttccatctt tgataacaaa aataaaggtc gagttttgat aataagcaaa tacatctgta
    15901 ataggtggct ttgttaaaaa aacggtaagg gattgagatg ccgttttccc ttttttatcg
    15961 gtaacaacaa atgataacaa atattctcct tctgtctggg gcagttgaat atttaatgag
    16021 tcaacatact tagcagccga aacctggtca atagttttgt cttgccagct atctaaaaat
    16081 ccggctccgg ctttgataga aaaagttgaa aggtcggaag tccctttttc tgcaacccat
    16141 ttcactgtaa tagaagtacc tgcgctagca tttatagagt tgcctgaaat atttgaaccc
    16201 gaaactgtca gtgtagggcc tgcttctgca tcttttgtat tatcttcaca accaaccaaa
    16261 caagacacac aaaacaatgc aacagctaat ttaaataaca aatttgttct catggtttta
    16321 aaatttgtgg tttaggtcaa ttctgtctga atttttaata ttcaataaat atacaaaatt
    16381 ttacccacaa atactataag caattgattt aaaagtattt caatcgtcaa aatgaatgga
    16441 tagaatatta cattcatgtt tcacccaata tttgtatttt tatcacgttt ttttaaaaat
    16501 aactattaac ctgtaatttg atgaagataa caaataaggt ttattcaaaa acttcagcat
    16561 ttctgcatca ttcctttgta ttatcaatat tgctgggttt atttttgaat gcaaacagtc
    16621 aaacatctat aagcgacgta aaaccagata acttcagtaa tgctctggct cttttcaagc
    16681 agggaaaata tgcagcggca caagctaagt ttgaaagcat tgctaataat gatgtttcaa
    16741 aaagcaatga catcaaaaga gaagctttgt attactcttt tctttgttca caacagctat
    16801 tgctcaatga tgccgaaaat aaggggttag aattcattaa aacctacggg ggaagcagta
    16861 aagcaaatga tgtatatttc cagctgggtc ttaaccaata cgtttggaag cgttatccta
    16921 aggctataga ctattttgct aaggttaaaa aaagtgagct taccgaaagc cagcaatcag
    16981 aatatatttt caaatacggt tacagctttt tcatgctcga tagcttggaa aaagcccgca
    17041 aacagtttta cgaactgata gacatcgata cacgctacac cccatcggca atttattact
    17101 acgcccatat tgcctatacc cagaaaaact acgaaacagc attgcaaaac ttcttaaaaa
    17161 taaaagatga tgagatgttt gctgctatcg tcccttacta tatcagtcaa atctactata
    17221 tacaagaaaa gtacgatgct ttaattgaat atgcacaacc tttgctcgac tcagtcattg
    17281 aatcacgtgc agccgagatg aaccggcttg ttgcagatgc ctattatcag aaaaaaaact
    17341 acgataaagc attgacttac tataatgttt ttctctcgaa aaaatctgac ctcaaagctg
    17401 cagaatacta ccaacccgcc ttttgctatt atatgaagtc cgattttcaa aaagcaatcc
    17461 ctctttttga aaaagcagcc ttagacgaca gtcttaccgg gcaatacgcc aactatcaac
    17521 ttggagactg ttatttaaag accaatcaaa aacaaaaggc ccaaatggct tttgcttcgg
    17581 cagctaaaga cagcttcgac aaacaagtaa aagaagagtc aacttttaac tttgcagtac
    17641 ttacttacca attgtctgac aagcctttca acagcgccat caaagccatg accgattatc
    17701 ttgatgctta ccccaattct ctaagaaaag acgaggccta taattacctt atattggctt
    17761 gcatcaatac ccagaactat ggacaggcat tgaaatacct ttctaatgtc agaaaaaatg
    17821 agccaaaagt aaaaaaagca taccaaagag cttcattttt caggggattg gagttgtaca
    17881 acaatttgga atttgataca gcagccgttt tgtttgagtc ttccttaaaa tatgctgaac
    17941 aggagcctat tttggccgcc agggccaatt actggttagg cgaaataagc tttagaaaaa
    18001 agctatacga tgaggctctc gaatattaca accttttcat aaagtcgcct gtggcaaaac
    18061 aagccagcga atatagtatg gttagttaca accaggccta ttgctacttt aatatgaaaa
    18121 actactcggc agccaacgaa aacttccaaa aattttataa aaccgagaaa cctaaaaaat
    18181 caaaactcta tgccgatgct tgctctcgca ttggcgattg cattttctct acaggccagt
    18241 atagtgaagc tataacatat tacaatgaag ctatcgtggc tgatgccgct gacaaagact
    18301 acgcaacatt tcagaaagcc tttgctatgg ggcttatggg taaacaccct caaaaaattg
    18361 agattttaaa caacctgctt aaagacatgc cacaaactaa actggcagac aatgctttat
    18421 ttgaaattgg ccgcagccat gtggttttac aaaacaatcc taaagcagtc aaaagcttta
    18481 aaagtattat caaggattac ccatcgagca gctttgttcc aaaagcaatg ctccagcttg
    18541 gcatgattga ttacaacgat ggccgcaacg actcagctat tgcaaggtac aaacaggtta
    18601 ttgcgttata tcctgccacc cccgaagccc gcaatgcctt gaatggtatc cgcaccatca
    18661 tgattgaaca agaccagctc gatgccttta tggcttacgc cgaaactttg gggagctttg
    18721 caaacattac ccaatcggca caagactctc tgctgttttt ttcggccgag aaaatataca
    18781 cctcaagccg ctttgttgaa gctatagacg catttcaaaa atatatagac aaattcccat
    18841 ctggaaattt cattatcaat gcatggtatt tacaaggaat ctgctatatt gctatgtcgc
    18901 gccagtctga tgccctaaaa aactttgaaa aaattattgc caaacccagg acaagcttta
    18961 cagaacctgc attgctggaa gcttcaaaaa tatacatgag caaaaagaat taccaggaag
    19021 cccttgccta ctctattatt ctcgacagta tagccgaaac ccctgaaaat gcaatgattg
    19081 caaagttgca aaaaacagaa gcctattttg tactgcaaca atacaatgaa tgtatcacag
    19141 ttgcctcaaa attactggct ttaccatctt taacccctga aaatgaacga aaagcaaggt
    19201 attttcttgc caaagcttta tatatgaaca atgaagaggc taaggctacc gagcaattgc
    19261 gcagattagc caaagacatt aaaaatgccg aaggggccga agctgaatac ttgttaatca
    19321 aaatgcttta tgaacaaaaa cagccagaca aagcttacaa agaaatattc aaatttattg
    19381 accgcaatac ccctcatcaa tactggctgg caaaagcata cataattctt gcccaaattt
    19441 atgcagagaa aaatgacaag ttccaggctg tcaaaaccct cgaaagtatt attgaaaact
    19501 acgatatcag caatgatggg atattggacg aagctaagaa actaaaaaac aagcttcaaa
    19561 acaataaaac tcaaaaatct tctgatgatg agtttaaaat agccgaacca attaatgatt
    19621 tgcaataaaa ttaaaaacaa tgaactgtaa tatttccaaa aatataatct tcactggtat
    19681 tatagctttt gcaagcatgt ctttaacagg tcaggacctg aaaaaagaca tcacagttac
    19741 caaatcatat atcccaacta ttcaggaagc tgccaagatt aaccaaatgc cggtatttaa
    19801 tgataccggg tatgttgaac ctgattacaa ataccaaatt aagcccttag catttcattt
    19861 gccatatcaa ataaaaccct taccttctgc caagatggcc aaagaaacat gggacaatgt
    19921 ttacaacaac tatgcacttc ttggcttcgg aaacccttat tctccattag ctgaaattag
    19981 tatcaacagt ggccggacaa aaaattacat gtatggcatt aatttgtacc atcattcggc
    20041 caatggaaaa gtacgcctga aaaaagaccc tgattataaa gttaatgccc ccttttcttt
    20101 cacctctgtc aacggaaatg gcaaatatat actcgattac gcctctatac aaggcaacat
    20161 tggatataac catcatcgtt tggcctatta cggagtggac acagcattct taaacagtta
    20221 taaagaggat tccaataagc aattttacaa caatgcttat tttgacctgc ttttcaaatc
    20281 aaccaacctg gcttatgacc aaccagacta tattgccaaa acgtcatata actttttctc
    20341 agataaaaca ggttattctg agaataactt ttcgctaaac gctacataca tgcaccctta
    20401 taagaaatta aaacttggag gttcaattct ttaccaggtt tctgagcctt cacaacctgc
    20461 tgacagcggg tcaagttcta tctttctttt taaaccaacc gttacaaaaa aaatacaaaa
    20521 cgttaatatc agcgcgggtt tgaatatctg ggttgacaat taccaatcac tctcgaccat
    20581 ttattcacag ccttttgctt cgttagacgt ggtggttgtc ccagaagtat tacaagccca
    20641 attgggctac gaaagccaac tacagctaaa caacgccact tacctgatta ctgtcaaccc
    20701 tttcatgaaa tcgggaatta catcacctac tacatattct aagaatattt tcagcgctaa
    20761 tatcacagca gacattagca ataccgcaac aatggcattg gcagtatcgt acagcaacaa
    20821 taaagaccag gttttatggc gcaatgatgc aacatcaaca ttgaaaaaca agtttgtacc
    20881 tgtaatagac gacgttgaaa ctatcaaagt aaagacttcc ttgattttta cgccgacaaa
    20941 aagcttcaat gccactatag aagctggatt caacaactac actaccttac gcgaaaagaa
    21001 agcctggggc ttaccttcat ttgaaggcaa taccaccata cgatacaaca tccaggacaa
    21061 aattatcagc accacacagg tgtaccttgt tggcccacgc tattacgaaa ctgttgcaaa
    21121 tacctcaaaa tcgctgcctg cctttgttga ctttaacgaa aacattgaat accaatatac
    21181 aaagctgttt agcatatttg tgcaagtcaa gaaccttact gcttctcaat atgaatactg
    21241 gaacaactat tcatcctacc gttttcagtt tatggccggg gtgagatatg tattttaaca
    21301 taattagtag aaaattaccc ttttaggcat ttctcatact ttgttcccca aacatttctt
    21361 gcctataatt cagccttagc cggcaaattt acaggcaacg ctgagttgct tactgacaca
    21421 tacataaaat acataacctt aaaaagaatt tataatcttt gaatttttaa catttttagc
    21481 acgatttttg aataaaatac aaataaatat acatcaactt tgtataaata taaacagata
    21541 ttcctttgat ttaccaaaaa gatgtagtac cattgcatac gaaaacagaa cgtatttcat
    21601 atatacataa tttattaaaa ataagcgcat tatggctaaa atgacaactc aggattacat
    21661 tgcactcgaa gataaatacg gtgcacacaa ctatcacccg ctcccggtag tgttggaaaa
    21721 aggcgaaggc ccttttgttt gggatgttga aggtaaaaag tattttgact ttctttcagc
    21781 ttattcggct gtaaaccaag gacattgcca tcctaaaatt atcaaagctt taaaagatca
    21841 agcagaaaaa ctaaccctta cttcaagggc tttctatagc aatactttag gtgaatacga
    21901 aaaatttgtt accagttact ttggctacga caaggtattg ccaatgaact cgggtgctga
    21961 aggtgatgaa acagcattaa agctttgccg taaatgggcc tacaaagtta aaggcatcaa
    22021 agacggccag gctaagatta tagtatgtga tggcaacttt cacggacgta ccattactat
    22081 tgtttcgatg agtaccgacc ctgattctta tggtggattt ggccctttta ctcctggttt
    22141 tatcaaaatc cagtacaaca acctcaatga gcttgcaaaa gcattggaag acccggatgt
    22201 agcaggtttt cttgttgaac ctatccaggg cgaagctggt gtttatgttc cagatgccgg
    22261 atacctcaag aaagcttacg aaatgtgtaa agctaagaac gtattgttca ttgcagacga
    22321 agtacaaaca ggcctttgcc gtacaggtaa aaagctttgc tgcgaccatg aaggcgtccg
    22381 tccggatatt ttaatccttg gaaaagctct ttctggaggt gtaatgccta tttctgccgt
    22441 gcttgctgac gatgaaatta tgcttaccat caaacccggc gaacatggtt caacttttgg
    22501 aggtaacccg cttgcttgta aagtagctat cgcagccctt caggttcttg aagaagaaaa
    22561 actggccgaa aatgcagaac gccttggtaa aatattccgc gatgaaatta gcaaaataca
    22621 atctccactt atcgaaattg tccgtggtaa aggtttgctc aatgctgtgg ttataaaacc
    22681 tcacaaagga aaagaagcct gggatgtttg tgtagctttg aaagaaaatg gtttgcttgc
    22741 taaaccaaca caccagcaca tcatccgttt tgccccccct ctggtaatca acgaagctca
    22801 aattcgtgaa gctattgaca tcattggcaa aacaattaag tcatttgaat aaaagttaat
    22861 atgtatttcc aaaaagctgt caaagtataa attgacagct ttttgtattt ttgtactatc
    22921 ctcattgtag tatatttata gtaaatgtat tgcattttaa tagtatccct aattgtttct
    22981 attggccttt ttgctcaata tttttgtata aaaaaacatg acaaaaatta tagctgtttt
    23041 attgtttcta acgactcttt gtgttaaagg ccaggaatca aaataccagt taatatgggc
    23101 agatgagttc aatcaaagtt atttagatac caattattgg actattgaag tcaatggcca
    23161 tggtggcgga aataatgaat tacaatatta tacagatagg gcttcaaata tcaggctgga
    23221 aaacggtctg cttgttatag aagcccatca agagccgttt ctggaaaaac aatacacctc
    23281 agcccgcata aaaacgcaga aaaaagcctt ctggaaatat ggacgctttg aagcccgcat
    23341 gaaattgcct tacggtcaag gaatgtggcc agcattttgg atgctcggag aaaatatttc
    23401 ttctgtagga tggccaaagt gtggcgaaat agatatcatg gaaatgattg gcggaaatgc
    23461 caatgattca accgtatatt ctaccctgca ttgggcaaat atcgaagatg gaggccataa
    23521 acaacatggc gacacgctga aacttatctc tggcaaactt gctgacgatt ttcacgttta
    23581 tgctgttgaa tgggacgaaa aaacaataaa agccttttgc gacaacgcct gtttctttat
    23641 aatagatatc aatagccctg aaatgcacgc ttttcataac aattttttta tcatccttaa
    23701 ttatgctgta ggcggcaact ggccaggcag cccaattgaa accaccgttt ttccccaaaa
    23761 aatgcaggtg gactatgtca gggtttataa aaagcatgat taacagatat tttgcagata
    23821 ctcatttctt atcagactga tatttaggga caaacttttc taatttaagt atattttata
    23881 tctttagccc attaatacta aaataaaaga aaaatgctta ccaaaaaatt attatacgtt
    23941 gcgttatcaa caatggtatt ggcttgttgt acccccgaag aaggtgaaga ccttgttgac
    24001 ccaaacaatc ctccggccaa agaaatcgaa tacggcatgg aagccaaaat tggtaaaact
    24061 tcatggaaag cagtcaattt tagcatacaa atgattggca aaaatctgat catcagcggt
    24121 acctctgata caatgggcac tattaccatt catttgcaag atactgctgt tggtacctat
    24181 tatctcaatc ctgcttctgg caactatgcc tttgtagccg ataccatctc tgatgcatta
    24241 ggctatacgt ttggcaattc gcttgctggc ggggcggtaa taattaataa agtggacaga
    24301 gattcgttaa ctatttcggg aaacttcatt tttactgctt acaatggggt tcgccacaca
    24361 acaactacat attcgcaagg ctcgtttatc aatatccctt attctgaaac aagtttgccc
    24421 aaaaaaggga tgatttcata cattaacaac aaaaaagtaa ctgctgatag tgttaaagct
    24481 ggcattgcta aggatgaaat aatgtttatg gcttactttg gcaacgatga aattggcata
    24541 gatattaaaa atgtttttaa aggaaattat cctcttggca aagaatatgc gcatttggcc
    24601 cgttacaata ctataggcga tacgatttta tatactacta aagataataa ttatgttggt
    24661 ggcattgtat ctattaccaa tattaccaaa gattcattag catccggaag ctttgttttt
    24721 actgtttaca gggcaaaaga ccgcaaaata atgaatttca accgtggaat attcaacgac
    24781 attaaactta acaattatac caaataaata atggcatata cggctaacga ggcaaggtac
    24841 aacaacatgg tgtaccgcag gtgcggaaaa agtgggataa agttgccgat gatatctttg
    24901 ggcctttggc acaattttgg aggcgttgat gtttttgaaa atagccgcga aatcctttgg
    24961 catgcctttg ataacgggat aacccatttt gaccttgcaa ataattacgg cccaccagct
    25021 ggttcagccg aagaaacttt tggacaggtg atggataaag actttcgttc atatcgtgac
    25081 gaaatgatca tttctactaa agcaggctat tatatgtggc caggtcccta tggcgaatgg
    25141 ggctcacgaa agtatcttgt ctccagctta gaccagagcc tgaaaaggat gaagcttgac
    25201 tatgtggaca tattctattc gcaccgtcca gaccctgaaa ccccgataga ggaaaccatg
    25261 atggcacttg accatgtagt gcgtcaggga aaagctttat atgctggtat ttcaaactat
    25321 acagccgaac aaacacaaaa agctgctgag attcttaaaa gtcttggaac tccttgcctg
    25381 attcaccaag ccagatactc gatgtttgac cgttgggtcg agaacgcatt gttagatgaa
    25441 ttagaaaacc aaggtgcagg attgattgct ttttcgccac tggcacaggg gttgcttacc
    25501 gataaatatt tacatggcat tcctgaaggt tcgcgagctt caaaagagca tggttttctt
    25561 aaacctcagc atattactga cgaaaagttg gataaaataa aacagctcaa ccaaatagca
    25621 ttagatagag gccaaagcct tgcccagatg gctattgcct ggttgctcaa agacaacagg
    25681 gtgacttcaa tattggttgg cgtaagcaaa aaggaacaac tttacgactg tatcaaatct
    25741 atcgaaaaac ttgaatttgc taaagacgaa ttgcaaaaaa tagagaaaat actcggcgtt
    25801 tagttcaata ttttaaggta tatcaactcc aacctcttgt ttaccgtctt caaaattttc
    25861 acttggaaat ggtctatttc aaaaacctga ttttgggccg ggatgctatc taacttatac
    25921 accacaaaac ctgcaagcgt attgtaattt tcatcttcct gtatattgag ctggaattta
    25981 gagttcagcg tttcaatatc tattttgccc gaaaagatgt agctatcttc agaaagttgt
    26041 ttttcaacaa tgtcgttttt gtcgtgttca tcttctattt ccccgaaaat ttcttccata
    26101 atatcctcaa tagtaacaat acctgaggtg ccgccaaact catctaccac aaccgccagg
    26161 cttttccttt cctgcaaaag gctatgcagc aaattgttgg cattcatcgt ttccgggaca
    26221 aaaggagccg ggataattcg ggtttttatg ttattcgggt tttttataaa atctttaata
    26281 ttgatataac cgatgatatt gtcaatattt tctttataaa ccaaaagttt tgaaaaccct
    26341 gactcgataa acttattttt aacattgtca atagaagttt tgatatctac ggcaaccagt
    26401 tcagtcctgg gaatcataca gtcgcggact ttgacactcg aaaaatcaag ggcatttctg
    26461 aaaagtctta tttcatgttc ggtatcaatg tcctgaatct gtttatcttc ggtttgttcc
    26521 agcagatagg atatatccag cttgctaaaa acttctttgg tgtttttggt atcgctattt
    26581 ttgcccacca gctttagtat ccattccgag aacatcatga ttaattttgc cgggatgtac
    26641 aaaatgagat aaaaaacaaa caggggaaca cttaagtaat tcaaaaaaaa gttagggttt
    26701 aagcggaata taaacttagg gaagaactct ccggcagtaa ttataagcaa cgtagaaatg
    26761 atggtttgta aaacaaagac agacacttcg ttattgagta cttgcataag cggagccgag
    26821 agcatctttg ccattatcaa accatagata accaaaacca ggttattggc caaaagcact
    26881 actgcaatat attgcgaagg gtttttaaca aaaatctgta ctatgcccga tgtaaatatt
    26941 ccgcttttcc ggtccagctc aatccttagg cgattggccg aaacaaaagc aatttccatg
    27001 cctgaaaaaa atgctgaaac taaaaggcat aaaataacta aaaaccacga tgaggacaac
    27061 aattccataa aaggtcaatt taacagtata ttaaaatatt tttgacacac taacctgcaa
    27121 tcaacagaga atgatataac atccctttaa ttatctgaat aaataattgt ttcatttatc
    27181 agccaaaagt ttttatttgc aatcgtagtc aacaaaatta atgggtttct taatatgaaa
    27241 attctttttc gtacaaaaaa ctgattaatt tcaacatcaa tgattttcat ccccttgggt
    27301 aagacaccaa tcaaaatttt gttttgaccg ctaaacaaac atatttaaaa tttctttaaa
    27361 catattggat acacaaacac ttatgtattc tgtaataaaa taagtacttt tgcattttcc
    27421 gaataattat gaaagcatca tataaagtat caattatcat tgcaggtctg gtcttgctgt
    27481 cttttttggg ctatagcgtt gtttgtggaa gttctaccaa aaaagtaaat gaccaatata
    27541 tcattagtgc agccgagttg cctgaggctt tagcttttgc cggcgattct gttccgctcc
    27601 aaaagtttga tgttcgcgag agccttgacc gcgaaataca catcaatatt tattttcagt
    27661 cgcagaccat tttgatcctc aagcgcgcta accgtttttt gcctgagata gaacgaatac
    27721 ttaaagaaga aggggttcct gccgatttta aatacctggc agtagccgaa agtggtctta
    27781 ccaatgtggt ttcacctgct aaagctgcag gcttctggca atttctcaaa agcacaggac
    27841 aaaaatataa cctaaagata actgatgagg tagatgaacg ctatcatctc gaaaaggcta
    27901 ccagagcggc atgtcaatac ctgaaagagc tgtatggtat gtttggcaat tggacattag
    27961 ctgctgccgc ttataatgtt ggcgaaaaca ggcttaaaga tattttggca aaccaaaaag
    28021 ttagctcata ctatgacctt tggctcaatg aagaaacggc acgctatata tttaggattc
    28081 ttgctttaaa actaataatt gaaaaccctg ttgcttatgg tttttcgata ccagaaaaag
    28141 atctttatca acctttgcag tatattgagt taagtgttga ttctacagtt actgatttag
    28201 ctcggtttgc gttggcccaa aaatcgaacc tgaaaatgct cgtatatttc aatccttgga
    28261 taagaagcaa tagccttacc tgtaagacaa acgaaaaata tactattcgt atccctgctc
    28321 cgggttttag agaaagtgtt gagagcagca attatcagag ttatctgcaa caaaattgcg
    28381 acacgacaag caaacataaa actacatcat tttaacattc tcaaagcatt tacaaatggc
    28441 agataaggct ttagacaatc agtcgcagca agcatttatt gaagatccgg acaatattca
    28501 tgtgtatggg gcacgggtac ataacctaaa aaatattgat ttgtctatac cccgcaattc
    28561 attggttgtc atcaccggat tgagcgggag tggcaagtca tcgcttgctt ttgacactat
    28621 ttatgccgaa gggcagcgcc gctatatgga aacactgtcg gcttatgcac gccaatttgt
    28681 gggcaacatg gaacgccccg atgtggataa aattaccggg ctcagccctg ttattgctat
    28741 tgagcaaaag acaacaaata aaaacccccg ttcgactgtt ggcactatca ccgagatata
    28801 cgattttttg cgtttgcttt atgccagggc ttctattgca tattcgtaca acacaggcga
    28861 agcaatggtg cgctatagcg atgaccagat catcaaaatt atcaacgaga agtataaagg
    28921 aaaaaaaata tatcttttag ctccaattgt acggggacgc aaaggccact ataaagagct
    28981 ttttgaacaa atacgtaaga aagggtttat tcaagtccgt atagatggta aaatagaaga
    29041 actccgctat aacatgcgtt tagacaggta caaaacacac tatattgaga ttgttgttga
    29101 taagcttata gctggcgaag aaaatagtaa gcggataaaa gattctttag ctgtggcaat
    29161 gcgccagggt aaaggtatta tcatggtttt ggagaaagat gaagagaaag tacaatattt
    29221 tagccggctt ttgatgtgtc cttctaccgg aatatcatat aatgagccgg ctccgcatag
    29281 tttttccttt aattcgccac aaggcgcctg tcataaatgc aatgggcttg ggcaaattgc
    29341 agaacctgac atgaacaggc ttattccaaa tcctaaactg tctataaaga aaggtggtat
    29401 agcgccttta ggtacttaca agagttcata tatatttctt attatcgagg cattaggcaa
    29461 aaagtacgat tttacgctcg atacaccact tgagaatatt tcagaagatg ccctgaacac
    29521 cattttatat ggcacagacg agccattgca agttaaaagt gcttatggcg ggctttctaa
    29581 ctatactttg agttttgacg gccttatcaa ttatcttaat cagaatgctg aaaaagaaga
    29641 cgataccaaa aaaagcgagc tgaatttttt caaaaatgta gtatgccctg agtgtaacgg
    29701 cacccgtctt aaccgtgaag ctttgcattt taagattgac gggaaaaaca ttgccgagct
    29761 ttctgctatg gatatcgcag atttggccaa atggctcgag ggggtagaag accgtttgag
    29821 tgaaaaacag caagccattg cccgggaaat attgaaggag gtgaggacca gactgggctt
    29881 tctgctcaat gtaggtcttg actatcttac cctcgatcgt tcatcgcgga ccttgtctgg
    29941 cggtgaaagt caacgtatca ggcttgcaac acagattggc tcgcaattag tcaatgtttt
    30001 gtacatattg gacgaaccaa gtatcggtct tcacccgcgc gataataaaa ggctgataaa
    30061 atcgttgcaa aatcttcgtg atgcaaacaa ttcggtaata gttgttgagc atgaccgcga
    30121 aatgattgaa tctgctgatt atgttattga catggggcct aaagccggac gtcatggggg
    30181 cgatgtttgt gctcatggga aacccgaaga tttgaaaaat tattgttcta taacatcttt
    30241 gtacctgact aacaaaagaa aaatagacgt ccctgacaag agaagaaaag gaaatggcaa
    30301 aactatcacg ctgataggcg ctaccggcaa taatcttaag aatgtggaca ttgaaattcc
    30361 ccttggaatg atggtatgta taacaggcgt ttcaggtagc ggaaaatctt ctctgataaa
    30421 ccaaacctta cagcctattt taagccagca tttctatcgt tcgttaacag atcctcttcc
    30481 ttataaagaa attagaggcc ttgagaatat tgataaagtt atagaggttg accagtcgcc
    30541 tcttggccgt accccacgtt ccaatccggc aacttataca ggggttttta cagatattcg
    30601 taaattattt gagcttacgc ctgatgcaaa gatacgtggt tttgggccag gccgtttttc
    30661 ttttaatgta aagggcggac gttgcgaagc ttgtaaagga gctggcattc aaattgtcga
    30721 aatgaatttt ttgcctgata tttctgtcgt ttgtaaagaa tgtaatggca aacgttacaa
    30781 ccgtgaaaca ttggaagtac gttataaagg agaaactatc agcgatattt tggatatgac
    30841 cattgaccgt gccgtggact tttttgccaa cattcctgct atacaccgca aattagatac
    30901 cttgaaacag gttggacttg gatatattac acttggtcag caggctacta cgttgtcggg
    30961 tggtgagtca cagcgtgtaa agcttgccgc tgagcttgcc cgaaaagata ccggaaaaac
    31021 tttatatatt ttggacgaac ctactacagg gcttcatttt gaagacatcc gtattcttat
    31081 tgatgtactc aacaagctgg ttgagaaagg caattcggta gtcatcatcg aacacaacac
    31141 agatattatg aaggtagccg actatatcat agaccttggc cctgatggag gcaaacgtgg
    31201 aggaaatatt gtcaatgccg gcaccccgga gtttattgtt aaatctgcta aaggctttac
    31261 tgcacaatat ttgaaaaatg aattataata tttacccttg attattaaat tttgaaaaag
    31321 ttaaagaagt aagggcccga tgcaagagta acacgtttat ggttaataac tcaaatttaa
    31381 aattttagct tcctcattat tttttattag gggatattta ctttttctcc taaatatttg
    31441 ggcatcgttt gattgtataa atcccaatat accaacaaac gtgcaggaaa ttcgcgcaac
    31501 agtgttttta accccctgat aatacctacg ccggtagctc tgtatcctat agcattcata
    31561 cccatatatc gtgctatata tatagccctt tgaagatgaa agttttgcga tataataaga
    31621 acttcggttt gaccaaatat cgtcctgcac cgcactaccg agtcgaaagt cctgataccc
    31681 gcaaagtcaa gatatagtac cgaatccggg attcccatcc tgacaaggac atttctcatt
    31741 tttataggct cattgtaact catgtactga ttatcaccgc ttaggatgaa atatttcact
    31801 ttgccttttc gataaagctc tacggcagcg ccaatcctgt tgtagaaaaa tggattaggg
    31861 gacccttctt ttagtcggtt gcttgtcccc aataataaag ccactttggt attagggagc
    31921 ttgtttgtat ctgtataaat ataagggttt gattttaagg atatataaaa atatgatgtt
    31981 acaaggataa caatacctat agttgcaaaa aacgcaagta tttttatact tcttacaaag
    32041 cgaaatggtt gattttgagc gggtctcatt tatttgttct attgttgcaa aaatagttgt
    32101 ttttttgata cccgctttca atcggccaat aaatagaagt tattcttttt ttttgcagca
    32161 ggccggaagt ttgttgtatg ccttaaggcc tgcttttaca tcatcagcat cgtaccccag
    32221 ccagcttatt gcagctttta ttttgtctgc attggttttt gccggtttat aaactacagt
    32281 taaaattttt gtattcatct ccagatcaga actgactact cctttttcat aagcaagcgc
    32341 tgtctcaatg cgttctttac acatttcgca ttgtgcagat gtttgaattt tcaaggtatc
    32401 tgaactttgg gctttcataa agccagaaaa tgaaagcact acaactatta ataaaattat
    32461 ctttttcatc ttgtaagatt ttaacttgtt aatattcaat cgccatcagt catcagtaat
    32521 tattcaatct ttcaaagtat atcgtattcc ggcataaaac atacgtccca tgaccggccc
    32581 ccaaatcatt gatgcatcaa agtaattgct aaatggggca ttggctgcta ttacagggtt
    32641 cttttgcgta taattagtca ggttttcgcc ccctatatat acatcaaaat gcttgaaacg
    32701 acgtgttacc tgggctaata cgttgataaa agccggagaa tattgagcta attgatattc
    32761 agccgggttg gggctggtat taggcagtgg tgcacgaccg ttgaactggg tagttgcatc
    32821 aatttgccat ttgcggtggt taaaagcata agatgccgaa ataaggcccc tgtgggttac
    32881 aacaaaaggc ttgcttttca gttcaccgtg aacagtttgt tttacatcat tgatacgata
    32941 agctgtatta atttcaaggc ctttcaaagg atgaatattc aaatctgtct gaaatgcatc
    33001 agaataagat tttcctttca ggttgtaaac gattatcgat tgtggattgg catcaatgtc
    33061 aacaactacc tggttatcaa aggtagtatg gtagtagtcg gcagaccatt gaatgcttcg
    33121 gccttcaaca aaagcaaatg ttttagtaat attaaaacca taattccatg ctttttcgag
    33181 gtttagctgt tcggtaaaga tatatttgcg cgcactaacc aggtaattta aattttcggc
    33241 aatgacttcg gccgaatgca tgccacgccc aactgatgcc ctgagcacaa tgcccccaaa
    33301 tagctcatat ttaatatgtc cccttggtat taaataccat ccataacgtg aattgtagtc
    33361 gtaacgggag cctgccatag ctgtaaattt gtcagaaggc gaccacgtgt actgggcaaa
    33421 gaggccaggt acaaactcct gctttttaaa agccgaatca ttgagcaatt ggttgtaatt
    33481 gtcgtaattg gtgcttatcc ctgtgcttac tttatgagat gagttactta tcatcgtttg
    33541 ataaataaga tttgcataaa tactgtgttg acggccttta taatcattat accccatgaa
    33601 tgagttgtta tcataataag tatatgatga aatgaggcca aaaccattgt attcagtgct
    33661 gtcaactact attcctgttt ttgaatacag ttggaagttt tttattttcg actcaaatcc
    33721 gtaaatatct ttgtcaaggt cacttctttg gttgaaatcc atttgcccgc cttgcttttc
    33781 gtcattcaaa aaccggaaac caaattgttg ctcaacgtgt tcagaggtgt atttccaacg
    33841 gttgaatatg ttaatctgtc tgcctagggg ttgttcaata aatgagtcac cattttcatc
    33901 cattttattg aagtttcccg aaacatgggc catcaacatc ccgttccagt catgaccgct
    33961 aacaataacc ccgttggcat tgagctccat ccttgagtcg ttggctgcat aaacattaat
    34021 aaaaagaggg ggttcttcct caggtttttt gtattctata tttatttgtc cggttataga
    34081 ctcatatcct tgcaggatag aagatgtgcc tttagaaatc tgaattgcct ccatccacga
    34141 accggggaca taactcatcc cgtttacaat agataagccc ctcatcaaag ggatgttttc
    34201 aaacataagc tggctatata taccagaaag ccccaacatc tgtatttgtc gggcacctgt
    34261 aacagcatcg gcaaaaccaa catctacgct ggcattgctt tgaaagcttt cagagaggtt
    34321 gcagcaagcc agtctgtgaa gcccagcctg gctaataacc tctgttttca ttggttttaa
    34381 aacagaaata tagttacctt ccgttttttt tgttatagta acctcctcaa tttggctatt
    34441 ggcagacaat acaatggaaa taaattcttc ttcaggctta gttgctatgg tatctgcttt
    34501 atagcccaca taagaagcta taatattggt ttttccgtga ggtttattaa ttgaaaagct
    34561 cccatcttct ttggtttggg tgttgatggt ggtgttttcc caaaatactg ttgcatacgg
    34621 caatgccacc gaatcgcctt gctcgttaat ataatatacc ttgcctctca aaacgcttga
    34681 tgggtatgcc atagcaaagg tgagacaaag cgaaacgatt aaaataaaaa ttttgttcat
    34741 aatatattga gatttaaata aaacacaatg cataccagca tttgccaatt gtattttttt
    34801 acctggcaga tgaaataaac ttaaaaagct atgtattgat tagatctcaa caacgaaagt
    34861 tattttgcaa tataaataat aaaggggggc ttataaaaat agcaccagga gaaacctgta
    34921 tgttgaaaaa aactgttttt attagaattg gagaggtaat agtttttaaa taatgaacag
    34981 ccttaagttt taaaactttt tcaacggtat aaaggcttgt aatgtgtatg gtttgttctt
    35041 gatttgagca gcaagcttgt tgtataaccg tttcttttgc acttgttatg tttatttcgt
    35101 tacagcagcc atgattatca aaattgttta tggagtaaac acttttgccc aaagcaaggc
    35161 aagtatggaa attaatacta atgccaatgc ttgctgtggc aaagattagt atttggattg
    35221 acaatattat ttttccagtt agtttcacct ggctattttt tgcaaataaa acgttttcag
    35281 cccaattaag tttaagagta aaacatcttt ttatgttaaa tttattgaaa ttttgcacca
    35341 tggtttaata attgaaatca acctgaatca aaatcatttt ttaattttgg gaaataaaat
    35401 actttgcaca tgagacaatt cttattagtc ggtatttcac aatttttaat aatagcttct
    35461 ttcgcccaaa aaccagtttg ggttgatttt gaaaaacgta aacagcttta cccaaatgat
    35521 aaatatattg ttggctttta ttctgcccct aaaaaaacag acaaaccaga tgactcccaa
    35581 ttgcaaaccc ttttagaata tgctaaaact gatattattg aaaatatata tgtttcggtt
    35641 acttcggcca gcgaattaaa tacccggcaa acaggcaaag aatttatcca atcgttttcg
    35701 caacaaagcc tgatggcaac aaaagctgag cttgccggat taaaaaccga aacatggaaa
    35761 gataaaaaag agctttatgc tttagcatac gtgtcacgca ccgaattaat aagcttttac
    35821 aaaggaaagt tagctacaat gcttgctaat attgataaaa gtgtaactgc tgccgagtta
    35881 gaaaaaacta atggggataa agagaaagct ctggatatgt tttaccagtc tgccaagcaa
    35941 gtacgtgatg ccgaacaagc tgcagcaata ttgttgggcc taatgaaaca ggttcctgca
    36001 aacaacctgg cagatttgaa tacccgtatc aacaatggaa tcaatgctat catttcgaga
    36061 gaaaacccta cccctgacgc agcagcactt accattgctg ggatgctgaa aaaacagata
    36121 agtgctactg caccaaaggt taagattgca tcgcctacat ttcaggatac taagatggga
    36181 agcccttttt ctgcttactt ttcaaaaatg ttggaaagta agcttgggag ccgtggcttt
    36241 aacattgaac aaaataccca aaacaacagc gattatgtat tgaattgcac ctactggaaa
    36301 gaagcagata aactaaagct tatgctcaat atgtatgctg ttaagaccgg caaaataaca
    36361 gcaacggcag aggtctctgt gccacagtcg tggtttgcac aaaacaacat tgcatttatg
    36421 cccgaaaact ttgaagaaag ctacagccga gccaaagctt ttacaaaaga tgaaattgtt
    36481 ggaggcggct tgcgtattga tctttggacc aacaaatgtg ataacaaccc tgttttcttc
    36541 gaaaatgaca ctttgaaaat atacatgcgt gtaaaccacg aatgttactt gcgttttgtt
    36601 tattatatgg cagacgggtc aaaggtttta ctaatgaaca attattatat cgcttcggac
    36661 aaagtaaaca aagtgataga aatacctgaa agctttgtat gtgctgaacc ttttggcaac
    36721 gaaatacttg tagtcaatgc tcaaacagag ccttttcaga aacttaacac tcgcgaccag
    36781 gatggttaca cctttatcaa tgaaaatgta actgaagtag tagctaaaac acgtggtttc
    36841 acaaaaacaa caaaccaaac acttaatggc gaaaaacgtt tgaaccttac tacattgaaa
    36901 aaacaatatt gaaagtcatt gttgtccggt aaaaacagct cgctgctatg cagcttagaa
    36961 acatttggtg tattgggcta tcctacaaac agttcgcagc tacgctgcta ttatttagtc
    37021 gcatagcgat gtcctgtttg taataaaata aacatcgtta aaattaagcc ccagcggggc
    37081 gaccaatttc taatcaacaa tattcccagt atttataagt atctatttta ttttaccaga
    37141 caacagggat taaaagtatt attatctaca atagtagaaa tttattaggc tttatagcca
    37201 tttttatcgt gtaaaaatgg aaataataaa tctacaatag tagaaattta ttaggcttta
    37261 tagcctatat taaggagctg tcaatgacag ctgttgaatc tacaatagta gaaatttatt
    37321 aggctttata gcccccgata taactctgaa ttatctgaac gaaatctaca atagtagaaa
    37381 tttattaggc tttatagccg ccatcttacc cttactgtct gaccaattat ctacaatagt
    37441 agaaatttat taggctttat agccttacta atttaccatt tctgaaatag tatctacaat
    37501 agtagaaatt tattaggctt tatagccatc ttctacgtgc gcagaatacg tataatctac
    37561 aatagtagaa atttattagg ctttatagcc atccttcaga accaattcca ccaaatggac
    37621 atctacaata gtagaaattt attaggcttt atagcctgtc aatggttatt ttattgatag
    37681 ttctgatcta caatagtaga aatttattag gctttatagc ctttctttgc agtacaacat
    37741 tacaacgtat atctacaata gtagaaattt attaggcttt atagccaata gttttgaaac
    37801 tggctcaaga ttttatctac aatagtagaa atttattagg ctttatagcc acaacttaga
    37861 taaaagagct gaagcattaa atctacaata gtagaaattt attaggcttt atagcctcca
    37921 attgtgccca cataccattt aacaatctac aatagtagaa atttattagg ctttatagcc
    37981 ccccaacata aaaactatca ggcgaaaat
//
LOCUS       DAOSPF010000035        33965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1313_length_33965_cov_6.674314, whole genome shotgun sequence.
ACCESSION   DAOSPF010000035 DAOSPF010000000
VERSION     DAOSPF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_33965_cov_6.674314"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2205)
                     /locus_tag="PK252_08355"
     CDS             complement(<1..2205)
                     /locus_tag="PK252_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT14761.1"
                     /translation="MKRFLFFAVFSLLLFNGFSQSVAYLNPDLPFEQRVDSLVAQMTL
                     DEKISQMMDVAPAVERLGIPAYNWWNEGLHGVARAGYATVFPQAIGLAAMWNDSLMYV
                     IAKTIATEFRAKYNDAIKHNDRSRYKGLTVWSPNVNIFRDPRWGRGQETYGEDPYLSA
                     RYGVAFVKGLQGNNPKFYATIATPKHFVVHSGPEKLRHEFDIDVSQHDFLETYTPAFE
                     ACIKEGKAYSLMSAYNRFRGKSCSASDDLLNNLLRKKWGFEGFVVSDCDAVDDIYRTH
                     KIAKTPEEAAAISLKSGLDLNCGDCFSNLKVAIQKGLVTEADIDVSLKRLMMARMKLG
                     MFEPKGSVPYDTISMYSNDSKANRVLSLNAARQSIVLLKNNGVLPINRKTLTKIAILG
                     PNCEASRTMYGNYNGIPSKVVTPLEGLKAALLPKNKVYTNEMNGLVTEALSGKPVNAA
                     NLSFEGKPGLKAEIFNNLNFEGTPILTKIDTTIDYEWDSQFPAKGVSATNISIRWTGE
                     LTASETAEYYISFTGDDGFRVWIDNKLFLEEWKRQSPYTLTKAIKLNKGEKHAIKIEY
                     FQAGGGAVASLKWSYLDKTLADKVVAEAEKSDVIVYFGGLSPELEGEEMNVPYPGFDR
                     GDRTSIELPEVQRKMLKLLKKTGKPVVLVLMSGSAIGLDWENENLDAIVEAWYPGQEG
                     GTAIADVLLGNFNPGGRLPITFYQSTEQLPPFEDYSMKNRTYRYFTGKPLFPF"
     gene            complement(2238..3368)
                     /locus_tag="PK252_08360"
     CDS             complement(2238..3368)
                     /locus_tag="PK252_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT14762.1"
                     /translation="MRKLFFLALSALAVMTSCVNKDKSQERFIVKRGVNLSHWLSQDF
                     GWEPKYTYIKESDIRFIDSIGYDHVRIPIDEAEFWDEKGEPVQKAFEHLITCLDWCAT
                     YDLRAIVDLHIIRSHYFNATNEGGSNALWADTAAQSHFVDLWEKLSSVLNKYPTNMVA
                     YELLNEAVAPTDEDWNILYTKAYDAVRKLEPERTIVIGANMWQIVSNIPKMKIPAHDK
                     NIIISFHCYSPLVFTHYTAHWTPLKYFNGKVTYPGKIVSDEDLKNYLDTVKADVKGVL
                     SEVAQPFTKETFKEMFKPAVEFAKKKGLQLYCGEFGCLPTVPRADRLNYYKDIVSVFE
                     EQGIAWANWEYKGEFGIYTFDMEKKKSADPDFELIDVLLNAK"
     gene            complement(3433..3666)
                     /locus_tag="PK252_08365"
     CDS             complement(3433..3666)
                     /locus_tag="PK252_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14763.1"
                     /translation="MRYIVFLLVFLFTAGPIVENIQANTASHALMALASKKRKKPAKK
                     KKKDVKKKKKKKPAPKRKATKKTAKKNRVRYPF"
     gene            4002..4075
                     /locus_tag="PK252_08370"
     tRNA            4002..4075
                     /locus_tag="PK252_08370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4036..4038,aa:Pro,seq:tgg)
     gene            complement(4415..9211)
                     /locus_tag="PK252_08375"
     CDS             complement(4415..9211)
                     /locus_tag="PK252_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14764.1"
                     /translation="MPIHLHFKIKIIYLILLLGLSLDIFSTEQEKYLHFTEVDGLPRN
                     ITTCIEQDQYGYLWTGTTNGIAQYDGNSFYSYKELAGVSVFGLLYDSHNTLWAATGKG
                     LYKYNRLTNQFDIIVEGFIPKVQEFENDIYFIMYSGIGKIVGNKFEVFYKASDLADFF
                     ISKEGIWLAKSTDGVWLVNRNNKQNILAKYLQGKYVALINKIDDKLFVGCYNGQLYCI
                     PEDGKLVQLEFANHYFPKKFAKVGREIWLATDGNGIIVFDNNLKYIRTLSRSKNTEAS
                     VNSNSIYDIYPGKNNEIWLASFGAGLTCILPDNLLFQNILPEKGNENSLVANEGVSVF
                     VKQPLIYFGTNYGLSVWNENTNRFKNLSSEKLRKDLNGNKVTAINVDAYNNLWIGTYD
                     GLLGKYSSDLKLIKTFHPSSSSPNEMQQIVHIAEIDKSNLLILTQFQNNILLHFNIET
                     EKTDVFELNSKGSNITYLLINSLRKNQHGKLLALIADKGLYAVNWKENILENKFQEMN
                     KKLNSYMVDFYQDKKGRYWIASTSGLVCISADGKYFKRYTTKDGLPSNNLSRIEPEND
                     QYLWISSISGICRLNTQTDEVLNFNLNDGLPANEFYDRVSAKTNDGRIIFGSLAGFTI
                     IDPSKVNSGSSKADMIISDIVFQNQSIRNIEGIQLLKKPLEETKEIWLPYNKNSFSIH
                     FFKKDKNFLKYHNYAYRLKGLEKNWTYLTKTNFANFTNLSPGQYTFEVKSADKAKDGA
                     TTSIVIHIRAPWYLSWYAFLVYFIIFVTILYLSVYAYLKRLELKKEKEISEFKIQKEH
                     ELTEKKLSFFTNVSHDLKTPLTLIDAPVNDLLQSDNLNPEQVNKLMIISRNSKRLYKL
                     ITDLLDFRKVTQKQYVLEISETRISAIVADIYEAFKEECKHKEINFNYAVEENLTGYV
                     DASKIEKILWNLLSNALKFTGKGGQISLKIKEQIVDNQKYIKFEVVDTGIGIPENEKN
                     KIFDRFFKVQNSSLLNQDGTGIGLSIVKELVEIHLGQINVESTLGHGSTFTVIIPSGQ
                     EAYLHLNIEAPAIIGPVAQPIETKESTENLPLFENKKQYNLPTLLIVEDNSELRDYLC
                     KHFEKKYKVCSAKDGLEGLKQARDSSPDIIITDVQMPNMNGYEFCKEIRHNFDTSHLP
                     VIMLTANNTTEQQIEGLSTGADSYITKPFDINVLDAQVTSLTENRKLLRNKFLGIETP
                     ENLEKVLPQKDIDFILDLKLFIEENMMDQKLSIEMLSEHFALSIAQLHRKIKYIAGTT
                     PNNLIKSIRLKKAYQLIKEGRLRVSEAAYQTGFSDPNYFTICFKKEFGENPSKIAPFA
                     NETYNDTPVKLVSNEPANISKPEEPVSFEQQPLMLIAEDNEDMRNYICSELSSKYKII
                     AVADGWSAYEKAVEVIPDIILSDIMMPGIDGIELCRKLKTDDRTSHVPFVLLTAKDTD
                     ENTIEGLESGADDYIAKPFNIQVLKARLDNLILSRIALRKIFIKEPDATPKEISPSAH
                     DENFLKKAYEIVEKHLLDPNFDVHIFASELGVSRAQLYRKIDALSGQSVNEFIRIVRL
                     KKAADLLINSNFQISEIAEKVGFNSFAYFTKSFKEYFGATPSQYKR"
     gene            9516..12575
                     /locus_tag="PK252_08380"
     CDS             9516..12575
                     /locus_tag="PK252_08380"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT14765.1"
                     /translation="MKKIFIALPLLAMSFFVFGQNDSIVLADYETNSVYPARTYGWND
                     NWGLTIGVDNPTKDAVNPSEKVSSIHLTNQWGHAIVLNNFDEPVSLAMYPNVKLKVLS
                     DSAVSKKVGINIIDVNDFAVYAEVTLPADVNSKWANVVVNFAEKVTSTPVSFISKIEI
                     YIDRGTGDPAVGNKHNKYFVDDIRFTGPKANITETDMVTLFQESFSDNAWWMSEDYDG
                     CKSSGYKKLEDMRSIQWGGSALTAQSKFYNSTDSLLMVMVDWNGKGVAELRPFASRPG
                     LSSLVLKDIAIAGMKNMKLKYNLRWKEAPASYSLAPTVEYKVDGGNWAAVTTSSILPS
                     GTGTWANDIEYDFSAVSGKNISLKISNLTTAKCLLDEIRFIGNMVFADSVIVAGTDNV
                     SEINVDNGTLQMLDTIMPQIASQDVIWSVENGTGMASISQSGLLKAIKNGTVTVKARA
                     KDIWGTKVGKKTINLSNQIYYVDSISIKGKDGVFAINKSGDTLDIIIEAFPEAAHNKK
                     LNITLKPVGDKVAAVFENNKLIPVCDGQVYIVAQATDGSGIKDSALFTLTNQVPLKSI
                     AFTKQADTIKTVYGKLKLEVSFDPDTASNKSIVYSLISEGASAVITLSPNGNILPKAN
                     GKALVKVSSAVNPAIADTILIVVENYPVWKEFNVATKVDTTHISNNNDFSVKGKAAWD
                     SKYIYLHFDVTDDSIYAVGKESTWMLDNFEIYFDMNNNKTPAWPRGQSIWPSSFDGEK
                     GTHQLRMIPGMPFDSVNTTFKGWAVQKYEVTSSGYNFDLQITIDSLLKGYVPEVGKEI
                     GFDVLASDNDNAPYYRDQLSIFAPLGSIWCDAALWGTVRFAPDGGFEVVNDTIKPTEA
                     INLAATKGNKQVLLSWDAATDNIVVDKYVILVNNKPVETIYAQKTKNSYVVKNITPGD
                     YKFGVIAEDVAGNQSAQASISYTYTGVKGLEVNSFTIYPNPSSSFISFGNESMINVKI
                     FNAAGQMVLLKSVEPGERIDISSLKTGIYNVRIMGENSVSVLKLVKK"
     gene            12878..14458
                     /locus_tag="PK252_08385"
     CDS             12878..14458
                     /locus_tag="PK252_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT14766.1"
                     /translation="MNRLSILLLSCFLTITIYGQKSKPKTPERVINVDYSLEKGPLNT
                     MFNECVGAGRANEGLRADWQQQLTYIKNECGFRYIRFHGLLTDDMGVYGVDKNGKPQY
                     NYMYIDALFDFLLSINIKPFVELGFMPAALASGDKTIFWWRGNVTPPNDYERWGELVR
                     NLTAHFTERYGVDEVKTWYFEVWNEPNLTPYFWTGTREEYFKLYEYSAKAVKSVHPDY
                     RVGGPATAGASWEAELIDFCVKKNLPLDFISTHAYGVDQGYLDEYGNTGTRLAPNDLA
                     LSQDVLQSRKEIETSAKPGLELHYTEWSASYTPADPIHDSYHEASYVLHKLKQIGNAA
                     QSMSYWVFTDIFEEAGPRFTPFHGGFGMLNTQGINKPVFYAYQFLNRLGNIELINQDP
                     ASWVCKDKNGNVQVLAYDFTYTLPDDSINNQDYYIRDLPAKSKGKLKVNVSKLAAGKY
                     SLEVYKVGYRCNDAYTSYYDLGRPAQLTKLQVEQIKKINDGSPVGKEIITIQEGGVFS
                     KELDIRENDVYFLKLIKL"
     gene            14478..17114
                     /locus_tag="PK252_08390"
     CDS             14478..17114
                     /locus_tag="PK252_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT14767.1"
                     /translation="MRKNKILLSFLLCQVGLYAMAQTLSYNIRVNQVGYLPNAVKIAA
                     VADSKADSFKVMTSNLDSVVFRGQFLPPAYYNLSGEEVKLADFTLLQTSGSFVIVVDD
                     LGKSVPFSIDKNVFVNLSKASIKAYYYNRASTPILAQYAGVYAREAGHPDTAVVVLPS
                     AASANRPAGTKISTPGGWYDAGDYNKYIVSSGGTVATILSAFESFPAFYDTLNTNIPE
                     SNNNIPDILDEALWNIKWMMTMQDTDGGVYNKTTEANFSGFEMPSKVTATRYVCAKGT
                     AATLDFAAIMAMTARIYKKYNPQLADSALKQAIKAWQWAKKNPNKAFNNPSASGGYPA
                     VNTGGYGDNDFSDEFSWCAAELYITTKDSIYYKQMDLAKAYVLPGWPTVRTYGLLSLL
                     VNKDSLTSIADVDLLKSKLIDLVSGTKNNLFKTPYRIPGDFFYWAGNGAYANWGMLFM
                     QAYRITGNAAYYNAAISTLDYLLGKNATTYCFVTGQGTKSPMHPHHRISSADGVTEPV
                     PGMIVCGADGGDVADCGASKYPSTFSAKSYLDSECSYSTNEVAIGLNASFAFLAGAVQ
                     AEYLKTFIDSMPSYFSISTNKIDLPYKKGNDIELAIEGNLEWTLTPSADWITISTTDG
                     KGSGSVLINSQSNNPAESSRKGVVYVYNKNLLIDSITVTQMGIRKNFKIEAEDYSRMS
                     TVQIETTSDVGGGQNVGYVGKGSWVAYNIEITVEGVYNVTVRHAGYAGNFDIYIDSVF
                     VQNVVYPKTADWQVWDNFSTQLSFTEGKHDVMFKFNSDGINLNWYQFDWLWTLKVNSL
                     SNPGFINVYPIPTRAYLNIENNSTTSVNIQLLSMDGKVVKKQTMNGHAKERLDLSGIE
                     KGLYILEACSGWQKITKQVIVK"
     gene            17248..19278
                     /locus_tag="PK252_08395"
     CDS             17248..19278
                     /locus_tag="PK252_08395"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOT14768.1"
                     /translation="MKPLYISAICLFFLFTNAYSQQLYVGTNYHPHDSKTEQWKKDIA
                     LMKDAGFNVVRVGHLAWDSYEPSDGNFNFEWFDTVMNMLDQAGIKVILDIAIRPAPIW
                     LHQKFPSINVVDDNGSVRYPNHRYMEDAGDPNYQKYAIRFADVISKRYAKHPALLAFG
                     IDNESGDGPISYSETVRQRFIVWLKNKYGNLDKLNKAWATQRWSRRINSFDEVGLPVA
                     GNKNGAPEKILDFRRFVSDEVNQILFKVLDVVGKNAPNALTNTNAWYYSWMKYFDYSK
                     IAYSGKMTRHGCGFYPGNSLITNWGVMNAAFGIQRIQFESENPFWCNEFNTMTSVPNS
                     IRKSAYATLMYGNQMVCGWTWQSMWAGEEQYLQGMIDWDGVPNRKYDEYKKIAAEFKK
                     IEKYFPYKNKAEVGLAFSFASQIASGAFPVQHDGQIQACWDMFYWRNMDCKMLEISQS
                     SLKYKLLIVPGLAVMDELTANKIRAFIENGGTVIMTANSAIVDTTGQVFATTLPGLLS
                     DVFGIRVAGFEETEPMNEISRKGFKDKKLEFTYKNKAVETESARFDIIDAKSAKVTGL
                     ITSLDKDYPAMTLNKFGKGQAIYVGLPAKGEVLGNLLDDLIDELGIKKGPDVPSGIMA
                     RQIDEKHYLYLNVSAEPKEIKMRSKSKSILFDKEYNGNFTIAPYEPEFIEIK"
     gene            19305..21236
                     /locus_tag="PK252_08400"
     CDS             19305..21236
                     /locus_tag="PK252_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14769.1"
                     /translation="MNTKTNLLLTGTMILFCPFIFGQEKNCFLNDFVPKTAVIPASVN
                     ANKPTDKASVLVKLKNDTIGKVSKYVYGNAIVAWAGSYTNTEFVDGVKKLQPSLIRWP
                     GGSWADGWFLDKIPTDVPDSVYDGTKYTNSIAKTPKNKFGGHHGKEGGWVTTTDQYYT
                     LRQKTGVSEGLITVNYAYARYGTSKDPVSQAAHEAARWVRYDAGRTKFWEIGNENGGP
                     WEYGWMIDTTLNQDGQPTFMSGALYGKHFKVFADSMRNAAKETGATIYIGGQVMAGEA
                     TGGGQWGNGDRAWNEGFFKEVGDYADFYVIHNYFSNSTDVNSAITAAITSLRANAEYV
                     KKDIIKKNGISKPIALTEYNMNSGNDVLSNSYVMGMQAVALMCEMIKGNWGLGSRWNL
                     ETLFSGSGNYANHPHADFYYLYYLQKLYGDMAFSSTSSNKDVLSYTTKYSSSGDVAMV
                     IINKGNADQVVTVSSDSVGVGEKYYIYSFTAGTETTLSPYVYINENGPDFYHYGPYDG
                     LADIKAYAYTIDDEIKFTSPAKSLQMILIEAGNKKVVVNDPAFTAINSASGSTGITNY
                     PNPVGPSTLITYQLPKSSMVTLKVCNLQGKTVSVLVNQYQNAGYQAARLNAANLPAGI
                     YFYTIDAGSYHETRKFIKL"
     gene            21445..23964
                     /locus_tag="PK252_08405"
     CDS             21445..23964
                     /locus_tag="PK252_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HOT14770.1"
                     /translation="MAQTASLNPSGIVVEQPKSENDFPVVSANGTASAIYYDNAEYPG
                     VIRAICDLQNDIESVTGKKPAFITNNEVSDYEIIIGTLGKNKQIDNLVSSKKLDVKDL
                     KGKWESFVITTIANPAKNVKNSLVIVGSDKRGTIYAIYELSKQLGVSPWYWWADVPAQ
                     KRASAYIVSGRYAWGEPKVKYRGIFINDEAPCLTSWVKNTYGTDYGDHRFYARVFELI
                     LRLKGNYLWPAMWGWAFYADDTLNSKVADEMGIVIGTSHHEPMARNHQEWARKRAEYG
                     AWNYAKNQKVIDQFFKEGIERVQGKEDIVTIGMRGDGDEAMSKETDVQLLQRVVKNQR
                     AIIEQVTKRPAKETPQLWALYKEVLDYYEAGMRVPDDVIILLCDDNWGNVRRLPNEKE
                     RKHPGGWGMYYHVDYVGAPRNTKWLNVTPIQNMWEQLQLTYDYGVDKLWVLNVGDIKP
                     MEYPITLFMDMAWDPTRFSVKNLLEHPRAFCAQQFGDDQADEASRILNLYCKYNGRVT
                     AEMLDRNTYNLETGEWKKVSDEYLKLEAEALRQYLTLKPQYRDAYKQLILFPVQAMAN
                     IYEMYYSQAMNHKLYAENNPQANEWANNVERTFKRDAELSYDYNKVMANGKWDGMMTQ
                     KKIGYTTWNDNFPADKMPEVFRIENPENATGNYVFTPDNGYVAIEAEHYYTLKDAANA
                     KWTVLPYFGRTLSGMAVMPYSKPVDGASISYKMKLPDSVKTVTVHVVVKSTLAFANLS
                     GHRYQVGFNGADAQTINFNSDLNEKPENIYSIFFPTIARRIVEKKVKLNVPATTDGMK
                     VLTLTPLDPGIVFEKIVVDFGGYKNSYLFMEESTNKREVTK"
     gene            24129..26561
                     /locus_tag="PK252_08410"
     CDS             24129..26561
                     /locus_tag="PK252_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT14771.1"
                     /translation="MKRNTLHWFLILASLTLSIVTSYTQVHGQKQFSIERKQLFDYDW
                     NFFLGDTSSAQLNDFNDVAWRSLDLPHDWSIEGKILPKNVTGCAGGYFPAGIGWYRKT
                     FKAPVEWKNQKVSVYFEGIYMNSEVFINGKSLGVHPYGYTSFCYDLTPYLNFDKENVI
                     AVRVDNSQQLNSRWYSGSGIYRHVWLITTNPVHVAQWGVGITTSEVSTKKAKVQIKTL
                     VKNENSSSQSVIVKALLLDKNGKNVGNNQVKLDIAANSQKENIQTITVSNPLLWSPES
                     PNLYNAQIQILKEKQVVDNIKTQFGIRSISFSAENGFQLNGKTIKLNGGCVHHDNGCL
                     GAAAYDRAEERKIELLKAAGFNAVRTSHNPPSEAFLDACDKLGILVIDESFDGWKEAK
                     NKFDYSLYFDQWSTSDVEAMVLRDRNHPSIFMWSIGNEVYERKKPEAVEITKRLSAAI
                     KKIDTSRSVTSAMTTWDNDWAVFDPLMAAHDVCGYNYQLHRAADDHKLVPSRIIVQTE
                     SYPRDAFANWQLVQNNNYILGDFVWTAIDYLGESSIGRWYYSGDIPGEHWTQDFFPWH
                     GAYCGDIDLVGWRKPISHYRSMLYNSNEKLYLAVREPEPAPLEIKETLWSVWPTWESW
                     TWPGFEEKNVQVEVYSKYPKVRLYLNNKLVGEQPTTLEQQFKATFTVPYSPGLLRAVG
                     IENDKEQESTILQTAGEAAQIKLATDRKEIKANGQDLSFVTIEITDKNGIVQPNANNR
                     LIFKIEGEGTIAAVGNADIKDCDQYVGNARKAWKGRALVVVKSKRNAGTIKLTVSYLG
                     LPDATIDIKTIQ"
     gene            26593..27993
                     /locus_tag="PK252_08415"
     CDS             26593..27993
                     /locus_tag="PK252_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT14772.1"
                     /translation="MKIKFNLKQTLFKWAFLLVIGQTFISTIKAQLPTAQQIVDKMKV
                     GCNIGNTLEAICSETAWGAPKVTQILVDSIKAAGFNTVRLPVAWFCHSDTNSNIIDDA
                     WIARVKEVVDYCINDSLYVIINIHWDKGWLEKRVNAANQTQVNERQLAYWTQIAEYFK
                     DYDEHLLFASANEPDVSDANGMTILLSYHQTFIDAVRATGGNNSSRTLIIQGPSTDIE
                     KTNSLMNTMPTDQIANRLIVEVHYYTPYQFCLMDKDANWGKMFYYWGKSNHSTTDVTR
                     NATWGEETDVEKYFNMMKTKFVDKGIPVIIGEYGAYKRKLSPPSDQSLNDASVEYYHK
                     YIVKSSTSKGIIPFYWDTPGGLFNRNTGAVQDRGVLNAIMQGANEATGIASSIDMDIE
                     LYPNPFSSSFNLKISKPNEVVDISIFDFMGKLIKSVEKSNISGSMSINVPFGPGMYLI
                     QINGVNWIKSIKCIRI"
     gene            28045..30126
                     /locus_tag="PK252_08420"
     CDS             28045..30126
                     /locus_tag="PK252_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT14773.1"
                     /translation="MIKGLIIFAIVCSGFILDNTAQTKAPAPYGPVPTENQMRWQEME
                     YYGFIHFSLNTYTDQSWGYGNEEVKLFNPEKLDCRQWARICKEAGMKGVILVAKHHCG
                     FCFWPTETTEYSVKNAPWKGGKGDVVREMADACKEYGLKLGIYISPWDRNSPAYGKPE
                     YIQIFRKQIKEVLTNYGEIFEIWFDGANGGDGYYGGANETRKIDRTTYYDWKNTYAMI
                     RKLQPNIVIWNDGGDRGDLRWVGTEEGYVGETNWSLLNATGDVEWAMLHHGLENGNAW
                     VPAEVNTSIRPEWFYHPSEDAKVKNLPKLMNTYYNSIGRNGSLLLNFPVMPNGLIHEK
                     DEKAALDFAAAVKGSFAVNLAEKAKASASNVRGNSKDFSASNAIDGNKDTYWATDDNV
                     NTASLTIDFGKPVLFNRFMVQEYIRLGQRVKAFTLEAFVDGSWKEIAKATTIGYKRIL
                     RFSSVNATKIRFNITDSKSCPVISNVGVYNAPLILDAPVILRNQDGEVSISSDDIGPH
                     YYYTLDGSEPTPNSLRFTGPVKTNGKLVVKAIAFDPTSAKSSLMTQEKFDICRKDWKI
                     LGFSDENTRFILDGNPSTAWHQRDKKLPSDLIVDLGNTYEIAGFKYLPDQARWSAGVI
                     TNYEFYVSPDNVEWKKVDQGEFSNINNNPVWQIKQFAPEKARFVKLRALKTTWGDDIA
                     GYAEVDIITSK"
     gene            30890..32056
                     /locus_tag="PK252_08425"
                     /pseudo
     CDS             30890..32056
                     /locus_tag="PK252_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
     gene            32194..>33965
                     /locus_tag="PK252_08430"
     CDS             32194..>33965
                     /locus_tag="PK252_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT14774.1"
                     /translation="MAGNLFANIELLKGFTYNNNFGFDINFYEEDDFKPYYYITSTQN
                     NSVVQVYRKLDRNFSWNWQHVLDYKTTLFDKHNIDLMAGLEANEYLGKTLSGNADSLL
                     KNGATPEYQYIDATLRQTGSTYYYAKGGYSHQTGYAYFGRLNYEFNNLFLAQFTYRYD
                     GSTKFGPGHRFGSFPAFSTGFKFSELDFVKNSLPLLSFGKIRFGWGKTGNDGIPSNKF
                     YSLVGASAENGYPLGGTGTPGGIALAPGNSQLHWESITTYNYGLDLNFFDNKLSVTTD
                     YFEKYTTGMLQSLSLPLVTGRYGFDGSDGKYTDHIGSLSNKGIELALGYKDNVGDLKY
                     TVDFNITKIVSKVYDLTDTITLPDWESNPKAILRNGDAPGAFWGYKTDGLFRPSDVDT
                     IIENGKKRQVWKAGLPYKIDAQGRRVYAQNTAKPGDLRFVDTNGDTVLNDKDKVVIGN
                     PNPDFTFGFTINLEYKGFDFNCFFQGSYGNDIFNTSKASWYNPNGLNNWVKDALNAYR
                     DPVYDKNGVMIDPGNTTSNQFKLKGSTSDNYRMSDWYVEDGSYIRLKSIQIGYTLPEN
                     FTKKYGIERLRVYVGGRNLITWTN"
BASE COUNT        10637 a         6551 c         7029 g         9748 t
ORIGIN      
        1 aaaagggaac aaaggcttgc ctgtaaagta tcgatatgta cggtttttca tactataatc
       61 ctcaaatggg ggcaattgtt cagtcgattg atagaacgta atcggcaaac ggccgccagg
      121 gttaaaattg ccaagcaaaa catcagcaat agctgttccg ccttcctgtc cgggatacca
      181 tgcctcaaca atcgcatcaa ggttctcatt ttcccaatca aggccaatag cactacctga
      241 cattagcacc aaaacaacag gtttaccagt ttttttgagc aacttaagca ttttccgctg
      301 aacctcgggt aattcaatag aagttctgtc gcctctgtca aaaccaggat aagggacatt
      361 catctcttca ccttccagct cgggagaaag ccctccaaaa taaacgatca catcactttt
      421 ttcagcttcg gcaacaactt tatcagcaag ggttttatcc agatagctcc atttcaggct
      481 ggcaacagca ccgcctccag cttgaaaata ttcaatttta atggcatgtt tttcgccctt
      541 attaagtttt atagcctttg taagagtata aggcgattgt ctcttccact cttccaaaaa
      601 aagcttattg tcaatccaaa ccctaaagcc gtcatctccg gtgaaagaaa tgtaatattc
      661 agctgtttca gaagccgtaa gctcacctgt ccagcggata gaaatattgg tagcacttac
      721 cccttttgcg gggaactggc tatcccactc ataatcaatc gtagtatcaa ttttagtcaa
      781 gataggggtc ccctcaaaat tgaggttatt gaaaatttca gctttcaacc cgggttttcc
      841 ttcaaagctc agattggcgg cattaacagg ctttccggac aaagcttcag taaccaggcc
      901 attcatttcg ttggtataaa ctttattttt aggcaacaaa gccgctttta ggccttcaag
      961 aggagtaaca acttttgaag ggattccgtt gtaattgcca tacatagtcc ttgaagcttc
     1021 acaattgggg ccaaggatag caatttttgt caaagtcttc ctgttgattg gcaatacgcc
     1081 attatttttg agcaaaacaa tagactggcg ggcagcgttc aaagatagca ccctgtttgc
     1141 cttactatca ttagaataca ttgatattgt atcgtaaggc acagaaccct taggctcaaa
     1201 cataccaagc ttcattctgg ccatcataag acgtttgagc gaaacatcta tatcggcttc
     1261 agtaaccagg cctttttgaa tggcaacttt taaattagag aaacaatcgc cacaattgag
     1321 gtctaacccg ctttttagcg agatagcagc cgcttcttca ggtgttttag ctattttgtg
     1381 cgtacgataa atatcatcca cagcatcgca atcagaaaca acaaaacctt caaagcccca
     1441 ttttttgcga agaaggttat tgagcaaatc gtcagaggca gaacaagatt tgccacggaa
     1501 acgattgtat gcgctcatca acgagtatgc tttaccttct ttgatgcagg cctcaaatgc
     1561 cggggtataa gtctcgagaa aatcatgctg ggaaacatct atatcaaact catgcctcaa
     1621 cttttctggc ccgctatgta caacaaaatg cttaggagtg gcaatagtgg cataaaactt
     1681 ggggttgttt ccctgaagcc ctttaacaaa agctactccg taacgggcag acaaatatgg
     1741 gtcttcgcca taagtttcct gtcccctgcc ccatcgtgga tccctgaata tattgacatt
     1801 gggcgaccat acagtcagcc ctttataacg gcttcggtcg ttatgtttga tagcgtcatt
     1861 gtatttagcc ctaaactctg tggcaatggt tttagcaatt acatacatca atgaatcgtt
     1921 ccacatggca gccaatccta ttgcctgagg aaatactgtt gcatatccgg cacgtgccac
     1981 gccatgcagg ccttcattcc accagttata cgccggaata ccaagccttt cgacagcagg
     2041 agcaacatcc atcatctgcg aaatcttttc atccagtgtc atctgggcaa caaggctgtc
     2101 caccctttgt tcaaaaggca aatcggggtt taaataagca acactttgag aaaagccatt
     2161 aaataggagc aatgagaaaa ctgcaaaaaa taaaaaacgc ttcatatcat atcaaattag
     2221 taaagtattc aaatcaatta ttttgcattt aatagcacat caatcagctc aaaatcaggg
     2281 tccgcggatt ttttcttttc catatcaaag gtataaattc caaactcgcc tttatactcc
     2341 cagtttgccc aggcaatacc ttgttcttca aaaacgctta ctatatcttt ataataatta
     2401 agcctgtcgg cacgagggac agtaggcaaa cagccaaatt cgccacaata aagttgcagc
     2461 cccttctttt tggcaaactc aacagcaggt ttaaacatct ctttaaatgt ttctttggta
     2521 aatggctgag ctacttcact caaaacccct ttaacatcag ctttaacagt gtcgaggtaa
     2581 tttttcaggt cttcatccga cacaatttta ccagggtaag taaccttacc attgaaatat
     2641 ttcagtggtg tccaatgggc agtataatga gtaaagacaa gaggcgaata gcaatggaag
     2701 ctgataataa tatttttgtc atgcgcagga atcttcattt tagggatatt agaaactatc
     2761 tgccacatat tagccccaat gacaatagtc ctttcaggtt ctaattttct gacggcatcg
     2821 taagctttgg tgtacaagat attccaatct tcgtctgtcg gggccactgc ctcattgagc
     2881 aattcgtatg caaccatatt ggtagggtat ttgttcaaca cagaagataa tttctcccaa
     2941 aggtcaacaa aatgggattg agcagcggta tcagcccata atgcattaga tccaccttca
     3001 tttgttgcat taaaataatg agaacgtata atatgcaaat cgacaatggc acgtaaatca
     3061 taagtagcgc accaatcaag gcatgtaata agatgttcaa aggccttttg gacaggctcg
     3121 cccttttcat cccaaaattc ggcttcatca atagggatac ggacatgatc atacccaata
     3181 ctatcaataa acctgatatc gctttctttg atgtaagtgt atttgggttc ccatccgaaa
     3241 tcctgcgaaa gccagtggct aaggtttaca cctcttttaa caataaacct ttcctgcgat
     3301 ttatctttat taacacaaga agtcattacg gctaaagcac ttaacgctaa aaaaaacagt
     3361 tttctcataa tttaaagtgt tgtttaaatt acgaatttaa taaaagtaat catgatgaaa
     3421 cttagaaaga tgttaaaaag gataccttac gcggttcttt ttagctgttt tttttgtagc
     3481 cttacgtttg ggagcaggtt tctttttttt cttcttctta acatcttttt tctttttctt
     3541 ggcaggcttt tttcttttct tagaagccag tgccattaat gcatgcgaag cggtattggc
     3601 ctgaatattt tcaacaatag ggccagcagt gaaaagaaaa actaaaagaa agacaatgta
     3661 gcgcatacat attttttttg catagataca aaaataaatt tattagataa aataaaaatg
     3721 aataaaatca tctaacacat aagcacctaa gtaagctgaa atattaaacc caaattaagc
     3781 attaaaaatg cttaatcgat taaaaaattg aaattcaata atttaataaa acttttcaaa
     3841 caattctaaa tcagcttgta agaggtaaat gattggtata taaattagta aaacaaaaat
     3901 ttaaaataat gattttttta gaaaagaaaa attggtataa ttgaaaaaag tattattttt
     3961 gcgcctcgat tgatgagcga atcagttgca attaatagtt tcggggtgtg gcgcagttgg
     4021 ctagcgcacc tgctttggga gcagggggtc ctccgttcga gtcggagtac cccgacaacg
     4081 caaacagtaa ggcttgcaga aaggcaagcc ttatttgttt actaagtggg ttaagcatta
     4141 atccatttgc tttatttcat ccgtaaataa atttataaac aaggtcggat gaaccccgaa
     4201 gggctctccg aagaggaaaa ctcgcatgat gaattttcat ccttgtttgt gtttaaggaa
     4261 tcatgctcgt ttcatcataa taatatttcc ttttcaagga atgatagctt gtcccgtctt
     4321 tggcggaaga acacccctgt tcgctatttt ataattaaag tgtttgtgtt ttccaaacat
     4381 gggtgtttca tttttttaat aaaatacatt ttctttatcg tttatactgc gaaggagtag
     4441 ccccaaaata ttctttgaaa cttttggtga aataagcaaa tgaattaaac cctacttttt
     4501 ctgcaatttc agaaatctgg aaattagaat tgatgagtaa atcagcagcc tttttcaacc
     4561 tgacaattct tataaactca tttaccgatt ggcctgacag agcgtcaatc tttcggtaaa
     4621 gctgagccct gcttacgccc agttcagagg caaatatatg tacgtcaaaa ttggggtcta
     4681 acaagtgttt ttcaactatt tcatacgctt ttttcaagaa attttcgtca tgagcagaag
     4741 gtgagatttc ttttggagtg gcatcaggct ctttgatgaa aatctttcgc agggcaattc
     4801 gcgacaggat taggttgtcc agacgggcct ttaaaacctg gatgttgaat ggtttagcaa
     4861 tataatcatc ggctccactt tcaaggcctt cgatagtatt ttcatcggta tctttggctg
     4921 taagcaatac aaaaggcacg tggcttgtcc ggtcgtcagt tttaagtttt cggcaaagtt
     4981 caataccgtc tatcccgggc atcatgatgt cgcttagtat aatgtccggg ataacttcaa
     5041 ccgctttttc ataagcagac catccatcgg caacggcaat aattttgtat ttcgagctca
     5101 actcactgca gatataattt cgcatatcct cattgtcttc tgcaataagc atcaatggtt
     5161 gttgttcaaa actgacaggc tcttcgggtt tgcttatatt agcaggttca ttactgacaa
     5221 gctttacagg agtgtcgtta tatgtttcat tagcgaatgg ggcaattttg gatggatttt
     5281 cgccaaactc ttttttaaaa cagatggtaa aataattggg gtcgctaaaa cctgtttgat
     5341 aagctgcttc agagactctc aaacgtcctt ctttaataag ctgataagcc ttttttaaac
     5401 gtatagattt aataaggtta ttaggggttg tgcctgctat gtattttatt tttctatgca
     5461 attgggcaat acttaatgca aaatgttcag acagcatctc gatactcaat ttttggtcca
     5521 tcatgttttc ttcaataaaa agttttaagt caagaataaa atcaatatcc ttttgaggta
     5581 aaactttttc aaggttttcg ggggtttcaa tgcccaaaaa tttattgcgc agcagttttc
     5641 tgttttcggt caacgaggtt acctgggcat caagtacatt gatgtcaaaa ggcttggtga
     5701 tgtatgaatc agccccggta gagagccctt ctatttgttg ttcggtggta ttgtttgcag
     5761 tcagcatgat taccgggagg tgcgaagtat caaaattgtg ccggatttct ttacaaaact
     5821 catatccatt catgttgggc atttgaacat cggtaatgat aatgtcaggg cttgagtccc
     5881 tggcttgttt taagccttcc aatccatctt ttgcagagca aactttatat tttttttcaa
     5941 aatgtttgca caaataatcg cgaagctccg aattgtcttc aacaatcaat aatgttggaa
     6001 gattgtattg tttcttattc tcaaaaagcg gcaggttttc ggtgctttct ttagtctcaa
     6061 tgggttgtgc aacaggccca ataattgcgg gagcttctat gttgaggtgc agataagctt
     6121 cctgcccgga tgggataata acggtaaatg ttgagccatg tcctaaagtt gattcaacgt
     6181 tgatttgacc aaggtgtatt tctactaact cttttactat tgacaatcca atacctgtac
     6241 catcctggtt taacaaactt gagttttgga ctttaaagaa gcggtcaaag attttgtttt
     6301 tttcattttc agggatgcct atgccggtat caacaacttc aaatttgata tatttttgat
     6361 tatcaacaat ttgctctttt atctttagtg aaatttgacc accttttccg gtaaatttca
     6421 atgcattgga caataagttc cagagaattt tttcaatttt ggaggcatca acataaccag
     6481 tcaaattttc ttcgactgca taattgaaat ttatttcttt gtgcttacac tcttctttga
     6541 atgcttcgta aatatcagcc acaatagctg aaatgcgggt ttcggaaatt tctaacacat
     6601 attgtttttg ggtaactttt cgaaagtcca gcaagtcggt gattaatttg taaagccgtt
     6661 ttgaatttcg gctaataatc atgagtttgt ttacctgctc ggggttgagg ttgtcggact
     6721 gcaacaagtc gttgaccggg gcatcaatca gggttaaagg cgttttcaaa tcatgcgaaa
     6781 cattagtaaa aaacgaaagt ttcttttcgg tcagttcatg ttctttttga attttgaact
     6841 cagagatttc tttttctttt ttcagttcga gccttttgag ataggcatat accgaaagat
     6901 aaagtatagt tacgaagatt ataaaataca ctaaaaaagc ataccaactc aggtaccatg
     6961 gggccctgat gtgtattacg atgctggttg tcgcgccgtc ttttgcttta tcggcacttt
     7021 ttacctcaaa agtgtattgg ccgggggaaa ggttagtaaa attggcaaaa ttagttttgg
     7081 tcaggtacgt ccagtttttt tccaatcctt ttaacctata cgcgtaattg tggtatttca
     7141 ggaagttttt gtcttttttg aaaaaatgga tagaaaaaga gtttttgttg taagggagcc
     7201 aaatttcttt agtttcttcc aaaggttttt taagcaattg tatgccttca atattcctga
     7261 tactttgatt ttgaaaaaca atatccgaaa ttatcatatc agcttttgaa gaacccgaat
     7321 ttacttttga cgggtcaata atggtaaacc cggccaaaga accaaagata atacggccat
     7381 cattggtttt ggcagatacc ctgtcgtaaa attcgttggc agggagcccg tcgttgaggt
     7441 taaaattcaa cacttcgtct gtctgggtgt tgagcctgca aatgcctgag atactgctaa
     7501 tccacaaata ttggtcattt tcaggttcaa tccttgacag gttgtttgaa ggcagaccat
     7561 ctttggtggt gtatcttttg aaatatttac cgtcagcaga gatacacacc aggccacttg
     7621 tagaagcaat ccaatagcgg ccttttttgt cctggtaaaa atctaccatg tagctgttga
     7681 gttttttgtt catctcctga aatttgtttt ccaggatatt ttctttccag ttcactgcat
     7741 agaggccttt gtcggcaatt aaagccagca gtttgccatg ttggtttttg cgcaatgaat
     7801 ttataagcaa ataagtaata ttgctgcctt ttgagtttag ctcaaaaacg tcagtttttt
     7861 cagtttcgat attaaagtgc aataatatgt tgttttgaaa ctgggtaaga ataagaaggt
     7921 ttgacttgtc aatttcggca atatgcacta tttgctgcat ctcattgggg ctactgctgc
     7981 tgggatgaaa tgttttaata agtttgaggt ctgaagaata ttttcccaaa agcccgtcat
     8041 aagtaccaat ccacaagttg ttataagcat caacattaat ggcagtgact ttgttgccat
     8101 tcaaatcttt tctcaacttc tcgctactaa gatttttgaa gcgattggta ttttcgttcc
     8161 atacagaaag cccgtaatta gtgccaaagt aaataagcgg ctgttttaca aaaacagaaa
     8221 cgccttcgtt ggcaaccagc gaattttcgt ttcctttttc gggaaggata ttctgaaata
     8281 acaggttgtc tggcaaaata caggttaagc cagcaccaaa tgaagccagc caaatttcat
     8341 tgtttttgcc gggataaata tcgtaaatgc tattgctatt tacagaggct tctgtatttt
     8401 tgctacggct caaagtcctg atatatttga ggttattgtc aaaaacgata ataccgttgc
     8461 catctgtggc caaccagatt tcgcggccaa cttttgcaaa ttttttaggg aaataatgat
     8521 tagcaaactc aagttgtacc agctttccat cttccgggat gcaatataat tggccattat
     8581 agcagccaac aaatagtttg tcgtcaattt tgttaatcag ggcaacatat ttgccctgaa
     8641 ggtatttggc cagaatattt tgtttgttgt ttcggtttac aagccatacg ccatctgtgc
     8701 ttttggccag ccatatacct tcttttgaaa tgaaaaaatc ggctaggtcg ctggctttat
     8761 aaaaaacttc aaatttattg ccaacaatct tgccaatgcc cgaatacatg ataaagtaaa
     8821 tatcgttctc aaattcctgt actttgggga taaagccttc aacaataata tcaaactgat
     8881 tggtcaaacg gttatattta tacaagcctt ttccggtggc agcccaaagg gtattgtgcg
     8941 aatcgtacaa caggccgaaa acgctaacac ctgctaattc tttgtagcta taaaaagagt
     9001 tgccgtcata ttgggctatc ccgttggtag tccctgtcca taaatagcca tattggtctt
     9061 gttcaatgca agtggtgata ttgcgcggga gaccatccac ttctgtgaag tgcaggtatt
     9121 tttcttgttc ggtcgaaaat atatcgagcg agagccccaa taagagtatt aaataaatga
     9181 tttttatttt aaagtgcaga tgtattggca ttaacaacaa gttttagtga atgtatatga
     9241 agttgttgct tataagtgta atatgaaata gggcattaga gcgtaattat aatcattgtc
     9301 tcattgaaaa cgtgttaatt ttaatacaaa tgcgataaaa attcaaagat tttagagatt
     9361 attttcaaga ccatggagaa atacaagata tagttttggt taaaagctgg tttaaaaagc
     9421 ccactattta accaaatata ttgtttaact caaagtaagt tgatgttttt caaaagtggg
     9481 ggcatcagct ctttggacta ataactaacc aaattatgaa aaaaattttt attgctttgc
     9541 ctttattggc aatgtcattt tttgtattcg ggcaaaacga cagtattgtc ttagccgatt
     9601 acgaaacaaa cagtgtttat ccggcacgga cttatggctg gaacgacaac tggggtttga
     9661 cgattggagt tgacaaccca acaaaagatg ccgttaaccc gagtgaaaaa gtaagcagca
     9721 tacaccttac aaatcagtgg gggcatgcta tagtgttgaa caattttgac gaacctgtga
     9781 gcctggcaat gtacccaaat gtgaaactaa aagttttgtc agattctgca gtgtcaaaaa
     9841 aagtaggtat taatataatc gatgtcaatg attttgcagt gtatgcagaa gttacattgc
     9901 cagcagacgt aaatagtaag tgggcaaatg ttgtggtaaa ttttgccgaa aaggtaacct
     9961 ccaccccggt tagttttatc agcaaaatag agatttatat tgaccggggc acaggtgatc
    10021 cggctgtcgg caacaagcac aacaaatact ttgtggacga cattcgcttt acagggccca
    10081 aagccaatat aacagaaacc gatatggtta ctttgttcca ggagtcattt tcggacaatg
    10141 cctggtggat gtctgaagat tatgacggtt gcaaaagctc gggttacaaa aaacttgaag
    10201 atatgaggag tatccagtgg ggcggttcgg cattaacagc acaatctaaa ttttataaca
    10261 gcacagactc gttattgatg gtcatggttg actggaacgg caaaggtgtg gccgaacttc
    10321 gtccttttgc ttcacgcccc gggttaagct ctcttgtatt aaaagacata gcaatagccg
    10381 gaatgaagaa tatgaaacta aagtataacc tgaggtggaa agaagccccg gcaagttaca
    10441 gtcttgcacc aactgtcgag tacaaagtag atggaggcaa ttgggctgct gttacaactt
    10501 caagtatttt accatcgggt acaggcacct gggccaatga tattgaatat gattttagcg
    10561 cagtcagtgg aaaaaacatt agcctgaaaa tcagtaacct cactaccgca aaatgccttc
    10621 ttgacgaaat acggtttatc ggcaatatgg tatttgcaga tagtgtaatt gtagccggca
    10681 cagataatgt ttctgaaatt aacgttgata atgggaccct gcaaatgctt gataccatta
    10741 tgccgcagat tgcctcgcaa gatgtaatct ggtctgttga aaacgggaca ggtatggcca
    10801 gcatcagcca aagtggatta cttaaagcca tcaaaaatgg gacagtaaca gtaaaagcac
    10861 gtgctaaaga tatctggggc acaaaagtcg ggaagaaaac aatcaacctg tcaaaccaga
    10921 tttattatgt ggacagcatc tcgataaaag gcaaagacgg ggtatttgct atcaacaaaa
    10981 gtggagatac gttggatatt atcattgaag ctttccctga agctgcccat aataaaaagc
    11041 tcaatattac cttaaaaccc gttggcgata aagttgcagc agtatttgaa aacaacaagt
    11101 taatccctgt ttgcgatgga caagtatata ttgtggccca ggctacagat gggtcaggaa
    11161 taaaagactc agcccttttt acacttacca accaggtacc gcttaaaagc attgcgttta
    11221 caaaacaagc agataccatt aaaacagtgt atggaaaatt aaagctggaa gtaagctttg
    11281 accctgacac agcatcaaat aagtctattg tttattcact tatttcagaa ggggcatcgg
    11341 ctgtcataac acttagcccc aacggcaata tcttaccaaa agcaaatggc aaagccctgg
    11401 ttaaagtgtc atctgccgta aacccagcca ttgccgatac tatattgatt gtagtcgaaa
    11461 attatccggt ttggaaagaa tttaatgtag ccaccaaagt tgacacaacc catatcagca
    11521 acaacaatga tttttcggtt aaaggaaaag cggcctggga tagcaaatac atttatttgc
    11581 actttgatgt taccgatgat tccatttatg ctgttggtaa agaaagcaca tggatgcttg
    11641 acaattttga gatatatttt gatatgaaca ataacaaaac acctgcgtgg ccacgcggtc
    11701 aaagtatctg gccttcatct tttgatggag aaaaagggac acaccaattg cgtatgatac
    11761 ccggcatgcc gtttgattcg gtcaatacaa ccttcaaagg ttgggctgtt cagaaatatg
    11821 aagtaaccag ctcagggtat aattttgacc ttcaaataac tattgattcc ttattaaagg
    11881 ggtatgtccc tgaagttggt aaagaaatag gttttgacgt tttggccagt gataatgaca
    11941 atgctccata ttatcgtgac caactatcaa tatttgcccc gctgggttca atatggtgcg
    12001 atgcagcatt gtggggcacc gtaagatttg cccccgatgg aggttttgag gtagttaatg
    12061 acactataaa accaaccgaa gccataaacc ttgccgcaac aaaaggcaac aagcaggtac
    12121 ttctttcatg ggacgcagca acagacaaca ttgttgtaga taaatacgtc attttagtaa
    12181 acaacaagcc tgttgagact atctatgccc agaaaactaa aaattcatac gtggtaaaaa
    12241 atatcacccc gggagattat aaatttggtg taatagctga agatgtagca ggcaaccagt
    12301 cggcccaggc aagtatttct tatacttaca caggcgttaa agggttagaa gtaaatagtt
    12361 ttacaatata tcccaaccct tcatcatcgt ttatttcttt cggtaacgaa agtatgatta
    12421 atgtcaaaat tttcaacgcg gcaggacaaa tggtgcttct caaatctgtc gagccaggcg
    12481 aaagaattga tatttcttcg ctgaaaacag gtatttataa tgttaggatt atgggagaaa
    12541 atagcgtttc agttttaaag ttagttaaga aataaaatct tatttctaaa agacttttgg
    12601 agagggcaat tgagtcattc attgccctct ctttttgcta tgcggtaaag cctttaaaaa
    12661 acaggtacac gggagaatga aaacaacaat atgtaaacag cagatataca acaatctatc
    12721 gaattataat tattatctca tggtgttaga gtggattttt ataataaatg atagaaactt
    12781 aaagattttg gagatttttt tcaagagaca aagccttgca tagagaatag ttttgacttg
    12841 ttttcaaaaa gtgtataact aaataaaaaa taaaaaaatg aaccgtcttt caatattgct
    12901 acttagctgt tttttgacaa tcacaattta tggccaaaaa tcaaaaccaa aaactcctga
    12961 aagggttata aatgtagatt atagtttaga aaaaggcccg ttgaatacca tgtttaatga
    13021 atgtgtagga gcaggtcgtg ccaacgaagg tttaagggcc gattggcagc aacaacttac
    13081 ttatataaaa aatgaatgtg gtttcaggta catacgtttt cacgggctgt tgaccgatga
    13141 tatgggtgtt tatggggtag ataaaaatgg aaaacctcaa tacaactata tgtatatcga
    13201 tgcccttttc gattttttgc tcagtatcaa catcaaacct ttcgttgagt tgggctttat
    13261 gccggcagca ctggcaagtg gcgacaaaac aatcttctgg tggcgaggca atgtaacccc
    13321 tcccaacgat tatgaaagat ggggagagtt ggtgcgcaac ctcactgcac atttcactga
    13381 acgttatggc gttgatgaag taaaaacctg gtattttgag gtatggaacg aacccaattt
    13441 aaccccgtat ttctggactg gaacccggga agaatatttc aaactatatg aatacagtgc
    13501 taaagcggta aaaagcgtac accccgatta tcgtgtcgga ggccctgcta ctgctggcgc
    13561 ttcatgggag gctgagttga tagatttttg tgttaaaaaa aacctcccct tagattttat
    13621 cagtacgcac gcctatggag tagatcaggg atatcttgat gagtatggca atacaggtac
    13681 aagattagct cccaatgacc ttgccctgag ccaggatgtt ttgcaatcca gaaaagagat
    13741 tgaaacatca gctaagccag ggttagagct tcattatacc gaatggagcg cttcatatac
    13801 ccccgccgac ccgatacatg acagctacca tgaggcttca tacgtgttgc ataagcttaa
    13861 acaaataggc aatgctgccc aatctatgtc gtactgggta tttaccgata tttttgaaga
    13921 agcaggccct cgttttaccc cttttcatgg aggatttgga atgttaaaca ctcagggtat
    13981 caataaaccg gtattttatg cgtaccaatt tttaaaccgc cttggaaaca tcgaattgat
    14041 taatcaagac cctgcttcgt gggtatgcaa agacaaaaac ggcaatgtgc aggttcttgc
    14101 ttacgatttc acttacacgc ttccagacga ctctatcaat aaccaggatt attacatccg
    14161 cgatttgccg gcaaaatcaa aaggcaagct aaaagtcaat gtatctaagc ttgcagccgg
    14221 aaaatattca ttagaagtat ataaagtagg ataccgatgc aatgacgcat ataccagcta
    14281 ttatgatttg ggcaggcctg cacagcttac aaagcttcag gtagaacaaa taaaaaagat
    14341 caatgacggc tctcctgttg gaaaagagat aatcactata caagaagggg gcgtattttc
    14401 gaaagaactg gatatacgcg aaaatgatgt ttatttctta aaactaatca agctatagtc
    14461 tttataaaaa tataaaaatg agaaaaaata agattttact ttcgtttcta ttgtgccagg
    14521 ttggtttgta tgccatggcc caaacattaa gttataatat cagggtaaac caggtggggt
    14581 atttgcccaa tgctgtcaaa attgctgctg tggctgattc aaaggcagac agttttaaag
    14641 tcatgaccag caacctggac tcggtggttt ttcggggaca gtttttgccc ccggcctatt
    14701 acaacttatc cggcgaggag gtcaaacttg ctgattttac tttattgcaa acatccggca
    14761 gctttgttat tgttgttgat gatttgggca aatctgtccc tttttctatt gacaaaaatg
    14821 tatttgttaa tctttctaag gcaagtatta aagcatatta ttacaacagg gcttcaacgc
    14881 ctatacttgc ccaatatgcc ggggtttatg cacgtgaggc gggacacccc gacacagcag
    14941 ttgttgtact tccttcggca gcatcagcca acaggcctgc cggcactaaa atatcaacac
    15001 ccggcggatg gtacgatgca ggtgattata ataaatacat tgtaagctca ggcgggactg
    15061 tagcgacaat tctttcggca ttcgagtcgt ttccggcatt ctatgacaca ttgaatacca
    15121 atatcccgga aagcaacaac aacatcccgg acattcttga tgaagccctg tggaacatta
    15181 aatggatgat gaccatgcag gataccgatg gcggtgttta caacaaaaca accgaagcta
    15241 atttcagcgg ttttgaaatg ccctcaaaag taacagcaac acgttatgtt tgtgccaaag
    15301 gaacagctgc aactcttgat tttgctgcta ttatggctat gactgcccgt atttataaaa
    15361 agtataaccc gcaattggct gattctgccc ttaaacaggc tattaaggcc tggcaatggg
    15421 ctaagaaaaa ccccaacaaa gcatttaaca acccttcggc ttcaggtggc tatccggctg
    15481 taaataccgg aggatatggt gataatgatt ttagcgatga attttcatgg tgcgcggccg
    15541 agttgtacat tacaaccaaa gattcgatct actacaagca aatggacctg gccaaagctt
    15601 atgttttgcc tggctggcct actgtcagga cttatggatt gctgtcgctt ttagtcaata
    15661 aagattcgct tacatcaatt gctgatgttg atttacttaa aagtaagctc attgatttgg
    15721 tttcaggaac taagaataat ttgttcaaaa cgccttaccg cattcccggc gactttttct
    15781 attgggcagg taacggagct tatgccaact ggggtatgct tttcatgcag gcatacagga
    15841 ttaccggaaa tgctgcatat tacaatgctg ccatctctac acttgattat ttgttaggga
    15901 aaaatgctac cacctattgc tttgttaccg gacaagggac aaagagccca atgcacccac
    15961 atcaccgtat ctcaagtgca gatggtgtaa cagaacctgt tcccggcatg attgtttgcg
    16021 gcgctgatgg cggcgatgtt gctgattgtg gcgcttcaaa gtatccttct acattctcgg
    16081 ccaagtcata tcttgattcc gagtgcagct attcgaccaa tgaagttgcc attggcttaa
    16141 atgcttcttt tgctttctta gccggggccg ttcaagctga gtatttgaag acttttatcg
    16201 actctatgcc aagttatttc tctatttcaa ctaacaagat agacctgcct tataaaaaag
    16261 ggaatgacat agaattggct attgaaggta atttggaatg gactttaacc ccttcggcag
    16321 attggattac tatttctaca actgatggaa aaggcagcgg aagtgtgttg attaattctc
    16381 aaagcaacaa tcctgctgaa tcttcgcgaa aaggcgtagt gtatgtttat aataaaaact
    16441 tgctcatcga ttctattaca gttactcaaa tgggcattag gaaaaacttt aaaatcgaag
    16501 ccgaggatta tagccggatg tcaacagtgc aaattgaaac caccagcgat gttggtggcg
    16561 gacaaaatgt tggctatgtt ggcaaaggct cgtgggttgc ttataatatt gaaatcacgg
    16621 ttgaaggggt gtacaacgtt accgtcagac atgccggtta tgccggaaat tttgatatct
    16681 acattgatag tgtctttgtc caaaatgttg tatatcctaa gacagccgat tggcaggttt
    16741 gggacaattt ttcaacacag ttgagcttta ctgaaggcaa acacgatgtg atgttcaaat
    16801 ttaactctga tggcatcaat ttaaactggt accagtttga ctggctttgg acattaaaag
    16861 tgaattcact gtcaaaccct ggttttatta atgtttaccc tataccaacc cgggcatatt
    16921 tgaatatcga aaataattca acaacttctg taaatatcca attgctgtct atggatggca
    16981 aggtggtaaa aaagcaaaca atgaatgggc atgccaaaga aagacttgat ctttcgggaa
    17041 ttgaaaaagg gttatatatt ttggaagcat gttcgggttg gcaaaaaata actaaacagg
    17101 taattgtaaa atagcgcaaa ttactatata ttaataaatt gccattcgga acaataatag
    17161 ttgtaatgat tagttaggtg aattgaaatg ggaagggtaa gtaaaccttc ccatttgatt
    17221 aaaaattaaa aatctaaaat cttttatatg aagccattat acatttctgc gatatgcttg
    17281 tttttcttat ttaccaatgc ttattctcag caattgtacg tagggacaaa ttatcacccg
    17341 catgattcaa aaactgagca atggaaaaaa gacatagccc tcatgaaaga tgcagggttc
    17401 aatgtagtac gggtaggcca ccttgcctgg gacagttatg aaccttctga cgggaatttc
    17461 aattttgaat ggttcgacac cgtaatgaat atgctcgacc aagccggcat taaagttatc
    17521 ctcgatattg ccattcgtcc tgcgccaata tggctgcatc aaaagttccc gtccatcaat
    17581 gttgttgatg ataacgggag tgtcaggtat cctaaccatc ggtacatgga agacgcaggc
    17641 gaccccaact accagaaata tgctatccgc tttgccgatg ttatttcaaa acgttatgca
    17701 aaacaccctg cattacttgc ctttggcatt gataacgagt cgggcgatgg tccaatatcg
    17761 tattccgaaa ctgttcgtca aaggttcatt gtttggctga aaaataaata tggcaacctc
    17821 gataaactaa ataaagcctg ggccacacaa cgctggtcaa ggcgcattaa cagctttgac
    17881 gaagtagggt tgcctgttgc cggcaataag aacggtgctc ccgaaaaaat actcgatttt
    17941 cgccgctttg tttcagacga ggtaaaccaa atccttttca aagtattgga cgttgttggc
    18001 aaaaatgccc ctaatgccct gaccaacacc aatgcctggt attatagctg gatgaaatat
    18061 ttcgattatt caaaaatagc ttattcgggc aagatgacgc gtcatggatg cggtttttat
    18121 ccgggcaatt cgctgataac caattggggc gtgatgaatg cagcctttgg catacaaagg
    18181 atacagttcg aaagcgaaaa ccccttctgg tgcaacgagt tcaataccat gacctcggta
    18241 ccaaattcga tacgcaaatc agcttatgct actttgatgt atggcaacca gatggtttgc
    18301 ggatggacct ggcaaagtat gtgggcaggc gaagaacaat acctgcaagg catgatagat
    18361 tgggacggtg tgccaaaccg taaatacgac gaatacaaga aaattgccgc tgagtttaaa
    18421 aagatagaaa aatattttcc ttacaagaac aaggctgaag tggggctggc gttttcattt
    18481 gcaagccaga tagcaagcgg cgcctttccg gtgcagcacg atggccagat acaagcttgt
    18541 tgggatatgt tttattggcg aaacatggat tgcaagatgt tagaaataag tcaaagctct
    18601 cttaaataca agctcctcat tgtcccgggc ctcgcagtta tggacgaact tacagccaac
    18661 aaaatcaggg catttattga aaatggcgga accgttatta tgacggccaa ctcggctatt
    18721 gtcgatacta ccggacaggt atttgctacc acgctccctg gcttattgag cgatgtattt
    18781 ggcatcaggg tagcaggttt tgaagaaacc gaacccatga acgagatttc gcgcaagggt
    18841 ttcaaagata aaaagctcga atttacctat aaaaataagg ctgttgaaac cgaatctgcc
    18901 cgctttgata ttattgatgc aaaaagcgct aaagtaacag gccttattac cagtcttgac
    18961 aaagactacc cggcaatgac attgaacaag tttggcaaag gacaggccat ttacgttgga
    19021 ctgcctgcta aaggcgaagt tcttggcaat ctgctcgatg atttgattga tgaacttggc
    19081 attaaaaaag ggccagacgt tccatcgggt attatggcac gccaaattga cgaaaaacac
    19141 tatttatatc tcaatgtaag tgccgaaccc aaagaaatca aaatgagaag caaatcgaaa
    19201 agcatccttt ttgataagga atataacggc aattttacta tagcgcctta tgagcctgag
    19261 tttattgaaa ttaaataaat gataagcatt taaattacaa actaatgaac acaaaaacta
    19321 acttactgct aacaggaaca atgatattat tttgtccatt catttttgga caggaaaaga
    19381 attgcttcct caatgatttt gtgcctaaaa cggcagttat acccgcatct gtcaatgcca
    19441 acaagcccac tgacaaagcc tcagtattag taaaactgaa aaatgacacc atcggaaagg
    19501 tttcaaagta tgtatatggc aatgctattg tagcttgggc gggttcatat accaatactg
    19561 aatttgttga tggggtgaaa aaactacagc catcgcttat tcgctggccg ggaggaagct
    19621 gggccgatgg ctggtttttg gacaaaatcc ctacagacgt gccagactcg gtatatgacg
    19681 gaaccaaata caccaacagt atagccaaaa ccccaaaaaa taaatttggc gggcatcatg
    19741 gcaaagaagg aggatgggtt accactactg accagtatta tacacttcgt caaaagacag
    19801 gtgtttcgga aggtttaata acggtaaatt atgcctatgc acgctatgga acaagtaaag
    19861 accctgtatc ccaagctgca catgaagcag ccagatgggt acgctacgat gccgggcgta
    19921 caaagttttg ggaaattggt aacgaaaatg gcggcccctg ggaatatggc tggatgatag
    19981 atactactct caaccaggat ggacagccta catttatgtc tggagcactt tatggcaaac
    20041 attttaaagt tttcgccgac tcaatgagaa atgctgctaa agaaacaggc gctaccatat
    20101 acatcggggg gcaggtaatg gcaggcgaag ccacaggagg cggccaatgg ggcaatggcg
    20161 acagagcctg gaacgaaggc ttttttaaag aagttggcga ttatgctgac ttttatgtta
    20221 ttcacaatta tttcagcaat tccactgatg ttaatagtgc aataaccgca gcaatcacaa
    20281 gcctcagggc caatgcagag tatgtaaaaa aagatattat aaaaaagaat ggaatctcaa
    20341 agcctatcgc attaacagag tacaatatga attcgggcaa tgatgtactt tcaaattcgt
    20401 atgtaatggg tatgcaagct gttgctttga tgtgcgaaat gattaagggc aattggggct
    20461 tgggttcccg ttggaattta gagaccttat tttcaggttc gggaaattat gccaatcacc
    20521 cacacgccga tttttattac ttatactatt tgcaaaagct ttatggcgac atggcttttt
    20581 cttcaacgtc aagcaataaa gacgttttaa gttatactac gaaatattct tcaagtggag
    20641 acgtagccat ggtaattata aataaaggca atgccgatca ggttgttacg gtaagttcag
    20701 actcagtggg cgtaggtgaa aaatattaca tatactcgtt tactgcaggc actgaaacta
    20761 ctctttcgcc atatgtttac atcaatgaaa atggccccga tttttatcac tatgggcctt
    20821 atgacggact ggcagatatt aaagcctatg cttatactat tgatgatgaa ataaaattta
    20881 cttcgccagc caaatcattg caaatgatac tgattgaagc gggaaataaa aaagtcgtag
    20941 taaatgaccc tgcatttacc gcgataaact ctgcatcagg ctcaacaggc ataacaaatt
    21001 atcctaaccc tgttggtcca tcaacgctta taacttatca attgcccaaa agctcaatgg
    21061 taactttgaa agtatgcaac ctgcaaggca agacagtatc tgttttagtc aaccaatacc
    21121 aaaatgcagg ctatcaggca gctcgtctca atgcagccaa cttacctgcc ggtatatatt
    21181 tctataccat tgatgccgga agctatcacg aaacaagaaa gtttataaag ctttaatggt
    21241 gcttatcaga gatggcattt ttgtgcaagt aaacgttgtt tttatgtgta attttatcac
    21301 gcttaaaatt tataatatca actaataatc gacaatgtta tgattactac attaaaaaca
    21361 gcaaagttta ggaagaattt atctggcatc tttcgaaaat taatcctgat gattctcctg
    21421 aatatatcat ttattggtgt tactatggcc caaacagcca gcctgaaccc atctggtatt
    21481 gtagttgagc aacctaaaag tgaaaatgat tttccagttg tatctgccaa tgggacagca
    21541 tcggcaatct actacgataa tgccgaatat ccgggggtaa tacgtgctat atgcgatttg
    21601 cagaacgata tagaaagcgt tactggcaaa aagccagcat ttataaccaa taacgaggta
    21661 tcagattatg aaattatcat tggtacgctg ggcaaaaata aacaaattga caatttagtt
    21721 tcatcgaaga aactggatgt aaaagacctt aagggcaaat gggaaagctt tgttattacg
    21781 accattgcca atcctgcaaa aaatgtcaaa aacagtcttg taatcgtggg tagcgacaag
    21841 cgcgggacca tttacgcgat atatgaactt tctaagcaat taggggtgtc gccctggtat
    21901 tggtgggccg atgtaccggc tcaaaaacgg gcatcagcat atattgtctc tggtcgttat
    21961 gcctggggcg aacctaaagt aaaatatcgc ggtattttca tcaacgatga agccccttgt
    22021 ttgacaagct gggtaaaaaa tacatacggt accgattatg gcgaccatcg cttttatgcc
    22081 agggtttttg aattaatcct tcgcctgaaa ggcaattatt tatggcctgc catgtggggc
    22141 tgggcttttt atgccgatga cacgctaaac agcaaggtag ccgatgaaat gggcattgtc
    22201 attggtactt cccaccacga gcctatggca cgcaaccacc aggaatgggc ccggaaacgt
    22261 gccgaatatg gggcttggaa ttatgcaaaa aaccaaaagg taatcgacca attcttcaaa
    22321 gaaggcattg agcgggtgca aggaaaagaa gatattgtaa ccattggtat gcgtggcgat
    22381 ggtgacgaag ctatgtcgaa agaaaccgat gtacaactac ttcagcgagt ggtgaaaaat
    22441 caacgtgcta ttatagaaca ggttaccaaa cgccctgcca aagaaacacc ccaattatgg
    22501 gcgctgtata aagaagtgct tgattactat gaagcaggca tgcgcgttcc tgatgatgta
    22561 attatacttt tgtgcgatga caactgggga aatgtaaggc gcctgcccaa cgaaaaagaa
    22621 cgcaagcacc cgggcggctg gggtatgtat taccatgttg attatgttgg tgctccccgc
    22681 aataccaaat ggctcaatgt tactcctatc cagaatatgt gggaacaatt acagctgacc
    22741 tacgattacg gcgtagataa actctgggta ctcaacgtgg gcgacatcaa accaatggaa
    22801 taccccatta cgctctttat ggacatggct tgggacccga cgcgctttag tgtcaaaaac
    22861 ctgcttgagc atcctcgggc cttttgcgcc caacagtttg gcgatgatca ggccgatgaa
    22921 gcctcccgta tccttaattt atattgtaaa tacaacggac gcgtaacagc cgaaatgctt
    22981 gaccgcaaca cctataacct cgaaacaggc gagtggaaaa aggtttcgga cgaatacctc
    23041 aaactcgagg ctgaagcttt gcgacaatac cttacgctga aacctcaata ccgtgatgct
    23101 tacaagcagt taatcctgtt ccccgttcag gccatggcca atatctacga aatgtactac
    23161 tcacaggcca tgaaccacaa gctatacgcc gaaaataacc cacaggccaa cgaatgggcc
    23221 aacaacgtag aacgcacctt taagcgcgat gccgaactca gctacgacta taacaaggtg
    23281 atggccaatg gaaaatggga tgggatgatg actcaaaaga aaattggcta tacgacatgg
    23341 aatgacaatt tcccggctga taagatgccc gaagttttcc gtattgaaaa ccctgaaaat
    23401 gcaacgggaa attatgtctt cactcccgac aatggatatg ttgcaataga agccgagcat
    23461 tattacacgc taaaagacgc tgccaatgct aaatggaccg ttcttccata ttttggtcgc
    23521 acgttgagcg gaatggctgt aatgccttat tcgaaacctg ttgatggcgc ttccatctcc
    23581 tataaaatga aactcccgga cagcgtaaaa actgttacag tacatgtagt ggtaaaatca
    23641 accttagcat ttgctaattt aagtggtcac cgctatcagg ttggttttaa cggtgccgat
    23701 gcgcaaacca tcaatttcaa ttctgacctc aacgaaaagc cagaaaacat ttattccatt
    23761 ttcttcccaa ctattgcccg caggattgtc gaaaaaaagg tgaaattaaa tgtacctgca
    23821 acaaccgatg gtatgaaagt tttgacacta accccgctgg atccgggcat tgtatttgaa
    23881 aaaatagtag ttgactttgg cgggtacaaa aactcgtatt tgtttatgga agagtcaaca
    23941 aataaaagag aggtaacaaa ataacgagaa ataaaatgaa acacccatgt tcttgcaaat
    24001 gcaacttaac gaagcgtatt tataagcaag gtgaataaac acgatagtta taaaattgca
    24061 aacatgggtg tttcattgtt aaaaacggga gaagctatca tacctttaag aattaaaaaa
    24121 taaatatgat gaaaagaaat acgctgcatt ggtttttaat tctggcttca ttaacattat
    24181 ccattgtaac aagttatacc caggttcatg gacaaaagca gttctctatt gaaagaaagc
    24241 aattatttga ttacgactgg aatttttttt taggcgatac ttcttcagct caattgaacg
    24301 actttaacga tgttgcctgg cgtagtctcg acctgccaca cgattggagc atcgaaggaa
    24361 aaatacttcc caaaaacgtt acaggatgtg caggtggtta cttcccggca ggcattggtt
    24421 ggtaccgcaa aacattcaaa gccccggttg aatggaaaaa ccaaaaagtt tcagtgtatt
    24481 tcgaggggat atatatgaac tcagaagttt ttataaatgg caaatcactt ggggtacatc
    24541 cttatggcta cacttctttc tgctacgacc taacaccata tttaaatttc gacaaagaaa
    24601 atgttattgc agtacgggtg gacaattcgc agcagttaaa cagtcgttgg tacagtggtt
    24661 cgggaattta tcgccatgtt tggttgataa caactaatcc tgtgcatgta gctcaatggg
    24721 gtgtgggcat tacaacatcc gaggtatcaa cgaaaaaagc gaaagttcag ataaaaacat
    24781 tagtaaaaaa tgaaaattct tcatcacaaa gcgtcattgt taaagctctt ttattagaca
    24841 aaaatgggaa aaatgtcgga aataatcagg taaagcttga tattgcagct aatagccaaa
    24901 aagaaaacat tcaaaccatt acggtttcaa atcctttgtt atggtcaccg gaatcaccca
    24961 atttatacaa tgcccaaata cagattttga aagaaaagca ggttgttgat aatattaaaa
    25021 cccagtttgg gatacgttct atcagctttt ctgccgaaaa tgggtttcaa ctcaatggca
    25081 aaacaataaa gctcaatggg ggctgtgtgc atcacgacaa tggctgtctg ggcgctgcag
    25141 catatgaccg tgccgaagaa cgtaaaattg agctgctcaa agctgccggt tttaatgctg
    25201 tacgtacctc acacaatcca ccatcagaag ctttcctcga tgcatgcgac aagttgggaa
    25261 tattggtaat cgacgaatcg tttgacggtt ggaaagaagc aaaaaacaaa ttcgattatt
    25321 cgctgtattt tgaccaatgg agtaccagcg atgtagaagc tatggttttg cgagatcgca
    25381 accatccttc aatttttatg tggagcatcg gcaacgaggt gtacgaaaga aagaaacccg
    25441 aagcagttga aattactaaa agactttcgg cagccataaa aaaaatcgat accagtcggt
    25501 cggttacctc ggcgatgacc acctgggaca acgattgggc agtatttgac ccgctaatgg
    25561 ctgcacacga tgtttgtggc tataattacc agttacatcg ggcagcagac gatcacaagc
    25621 ttgtcccttc acgtattata gttcaaaccg agtcatatcc acgcgatgct tttgctaact
    25681 ggcaactggt gcagaacaat aactatatcc tgggcgattt tgtgtggaca gctatagatt
    25741 atttgggcga atcatccatt ggccgttggt attactcggg agacatccct ggcgaacact
    25801 ggactcagga tttctttccc tggcatggcg catattgtgg agatattgac cttgttggct
    25861 ggcgaaaacc catttcgcat taccgaagca tgttgtacaa tagtaatgaa aagctttatt
    25921 tggctgtccg tgaacctgaa cctgccccat tagaaataaa agaaactttg tggtcggttt
    25981 ggcctacctg ggaaagctgg acatggccag ggtttgaaga aaaaaatgtt caggttgaag
    26041 tttattcgaa atatccaaaa gtgcgattat acctcaataa taaacttgtt ggcgaacaac
    26101 caactaccct cgaacaacaa tttaaagcta cattcacagt gccatattcg ccaggtttgc
    26161 ttagagccgt tggtattgaa aatgataaag aacaggaatc aactattctg caaacagcag
    26221 gagaagccgc tcaaataaaa ttggcaaccg acaggaaaga aataaaagcc aacggacagg
    26281 atttatcttt tgttactatt gaaattactg ataaaaacgg aatagttcaa ccaaatgcta
    26341 acaatcgctt aattttcaaa atagaaggag aagggacgat agccgctgtt ggcaatgcag
    26401 atattaaaga ttgtgaccaa tatgtgggca atgcccgaaa agcttggaaa ggacgcgctt
    26461 tggtagttgt taaaagcaaa cgcaatgcag gtactatcaa attgactgta agttatttag
    26521 gtttgcccga tgccacaatt gatattaaaa ctatccaata atagatttcg aaattgttaa
    26581 aaaatatgta agatgaagat aaaattcaat ttaaaacaga cgctattcaa atgggctttt
    26641 ttattggtta ttggccagac atttatttca acaataaaag cccaattacc tactgcacag
    26701 caaattgtag ataaaatgaa ggtggggtgc aatattggaa atacccttga agcaatatgc
    26761 agcgaaacag cctggggggc gccaaaagta acccaaattt tggtagattc gattaaagct
    26821 gccggtttta acactgtaag gctcccggtt gcctggtttt gtcattctga taccaattca
    26881 aatattattg acgatgcttg gattgcccgg gttaaggagg ttgtagatta ttgtattaat
    26941 gacagccttt atgttattat caacattcac tgggacaaag gatggctcga aaaaagagta
    27001 aatgctgcca atcaaactca agttaacgaa agacagctgg cctattggac ccaaatagca
    27061 gaatatttta aagattatga cgaacattta ctatttgcga gcgccaacga accggatgtt
    27121 tcggatgcta acggaatgac gatattgttg agctatcatc aaacttttat tgatgctgtc
    27181 cgtgccacag gaggcaataa cagctcccga acgctaatta tccaggggcc ttcaaccgat
    27241 attgaaaaaa ccaattcatt aatgaacacc atgccaacag atcaaattgc caatcgtctg
    27301 atagttgagg tacattacta cactccatac cagttttgcc ttatggataa agatgccaac
    27361 tggggtaaaa tgttttatta ttggggaaaa agcaaccatt caacaaccga tgttacacgc
    27421 aacgcaacct ggggcgaaga aactgatgtt gaaaagtatt ttaacatgat gaaaactaag
    27481 tttgtagata aggggattcc tgttatcatt ggcgaatatg gtgcatacaa acgaaaacta
    27541 agccctcctt cagatcagag cctcaatgat gcatctgtag aatattatca taaatatata
    27601 gtaaagtctt cgaccagcaa aggaattata cctttttact gggacactcc cggaggcctg
    27661 tttaatcgca acacaggagc ggttcaggac cgtggcgttt taaatgcaat tatgcagggg
    27721 gcaaatgaag caacaggcat agcctccagt attgatatgg atatcgaatt atatcctaat
    27781 ccattttctt caagcttcaa tcttaaaatt agcaagccaa atgaagttgt tgatatttca
    27841 atctttgatt ttatgggaaa attgataaaa tcggtcgaaa aatcaaatat ttcaggttca
    27901 atgagcatca acgtgccttt tggcccagga atgtacctga ttcaaattaa tggggtaaat
    27961 tggataaaat ccatcaaatg tatcaggata taagtgtaaa atttttgact aaactaatgt
    28021 tgttcacatt ctaaaaaatc aaatatgata aagggcttaa taatattcgc aattgtttgc
    28081 tcaggcttta tacttgacaa tacagcccaa acaaaggccc ctgcgcctta cggaccagtt
    28141 ccaaccgaaa accagatgcg ttggcaggaa atggaatatt acggttttat tcatttttcg
    28201 ttaaatactt acaccgatca atcgtggggg tatggcaacg aagaggtaaa gcttttcaat
    28261 cccgaaaaat tagattgccg tcaatgggct cgtatttgca aagaggccgg aatgaaagga
    28321 gttatccttg ttgcaaaaca tcattgcgga ttttgctttt ggccaaccga aactacagaa
    28381 tattcggtaa aaaacgcacc ctggaaaggc ggcaaaggtg atgtggtccg tgaaatggct
    28441 gatgcctgta aagaatatgg cttgaagctc ggcatttata tttctccctg ggatagaaat
    28501 agccctgctt atgggaaacc tgaatatatt cagattttca gaaaacagat aaaagaagta
    28561 cttaccaatt atggcgagat ttttgaaatt tggtttgatg gtgctaatgg aggcgatggc
    28621 tattatggcg gggcaaacga aacccgcaaa atagaccgca caacctatta cgattggaaa
    28681 aatacttacg cgatgatccg taagttgcag cctaatattg ttatatggaa cgatggaggc
    28741 gaccgtggcg acttacgctg ggttgggact gaagaaggct atgttggcga aaccaactgg
    28801 agccttttga atgctactgg cgatgtagag tgggctatgt tgcatcatgg cctcgaaaac
    28861 ggcaatgcct gggtgccggc cgaagtcaat acttccataa ggcccgagtg gttctatcat
    28921 ccaagcgaag atgccaaggt aaaaaacttg cccaaattga tgaacacata ctacaattct
    28981 attgggcgca atggcagcct actgttaaac ttccctgtta tgcccaatgg tttgatccat
    29041 gagaaagacg aaaaagcagc gctcgatttt gccgcagccg taaaaggatc ttttgcagta
    29101 aaccttgcag aaaaagctaa ggcaagcgct tcaaatgtcc gcggaaactc aaaagatttc
    29161 tcggcttcca atgctataga tggaaataaa gatacttatt gggctaccga tgacaatgtc
    29221 aatacagcct cattgaccat tgattttggc aaaccagtct tgttcaaccg ctttatggta
    29281 caggaataca ttcgcttagg ccagcgggtt aaagctttca cgctggaagc ttttgttgat
    29341 ggcagttgga aagagatagc caaagctaca acaatcggat acaagcgaat acttaggttt
    29401 tcaagtgtta atgctactaa aatccgtttc aacattacag attcgaaaag ttgtcccgta
    29461 atttctaatg ttggtgtata taatgcacca ctaatccttg atgctcctgt tattttgcga
    29521 aaccaagatg gtgaagtaag catttcgtct gacgatattg gccctcatta ttattacacc
    29581 ctcgatggca gtgagccaac accaaattcg ttaaggttta caggcccggt caaaactaat
    29641 ggtaaattag ttgtaaaggc tatagcattt gacccgacat cagccaaaag tagccttatg
    29701 acgcaagaga aatttgatat ttgtcgtaaa gattggaaaa tattaggttt ttctgacgaa
    29761 aatacaagat tcattttgga tggaaatcct tcaacagcat ggcatcaacg cgacaaaaaa
    29821 ttaccttcag atttgattgt tgatttaggg aatacttatg aaattgccgg gtttaaatat
    29881 ctccctgatc aggcaaggtg gagcgctgga gttattacca attacgagtt ttatgtatcg
    29941 ccagacaatg ttgaatggaa gaaagttgac cagggcgaat tttccaatat caacaacaac
    30001 cctgtatggc agatcaagca atttgcgcca gaaaaagctc ggttcgtcaa gctacgagca
    30061 ttaaaaacta cctggggcga tgatatagcc ggatatgctg aagttgatat tataacaagt
    30121 aaatagcaat cataatcaaa agtcccggag ggacttaagt atggtagtaa taaggttcaa
    30181 atagtaatta tagtcccaaa cgggacgaaa gtgatttttg aaaaacactg taagccttat
    30241 atacaaaatt ctttaagttt atcttccgca cctacggtac ttttttaaac tgtatttgat
    30301 atttatctac catactattg accctccggg tctttcaaac ctaaagaata attttgttac
    30361 agatataaga tcactgttgc ccgttaaaaa tatcaggact aaagtccttc agtaagtatg
    30421 aatttgtaac cccagtctta aggctggggt tagtcaaagc tatgaattaa ttgggcttta
    30481 gccctattat tttactttat atcggacaac agtgatataa gacatcgtat ttgtaatatt
    30541 tctcaagcct ctttaattat tccgtgtaaa taatcaaaat attaataatg tctcattata
    30601 ggcgacaata tttataattc cgatagaatt tacaagattt tagagatttt tttcaagaac
    30661 ctcaaaagct aatcaaggta gttttggctc gatatattca atagtgaaag gacaattcca
    30721 atcacaggtt attattaacg ctgcatatac attgattttg aaggcatagt gagatggatt
    30781 tttataaaaa ccaattaaat aaataacaaa ctaatacaat taattaaaaa ctattaacta
    30841 acattttatg gaaaaaacag aaaaaagggc aaggcggttg ttgcctgcta tgaaaaaaaa
    30901 cctggcacta tttacggcca gtttgctatt gagtatcaca gctcttgcgc aagaacgcac
    30961 tatttcaggt acaattacag atgccacagg gttggggctt cctggtgtta atgttgttgt
    31021 aaaaggctca actaacggcg ttattactga tgttaaaggg aattatagca tcaaagtggc
    31081 tggggatgat gctgttttga ttttctcgtc tataggttat aaaaagacag aaatagctgt
    31141 tggaaaccaa acttcagtta atttatcaat gaaagaagat gttacagaaa tggaccaggt
    31201 agtggttatt ggttatggtg tacaaaagaa aagtgacctt acaggcgctg tggcatctgt
    31261 tactaatgac caattgctaa aaacccccag taccggggct gttcaggctt tacaagggaa
    31321 ggctgccggg gtgcaggttt tcaacagctc aggtatgcct ggtgctgcaa ttactgtcag
    31381 ggtacgtggt attaattcaa ttaccaaaaa agacgaatgg tctggagttt caggccctat
    31441 ttacattatt gacggtatcc cgggtgatat caactccatt aaccaaaatg acattgagcg
    31501 catcgaagtg ttgaaagatg cttcggctca agctatttat ggttcttccg gaggaaacgg
    31561 ggttatcatt gttacaacca aacaaggtgc taaaaaccaa aaaacaaaag ttgaattttc
    31621 aatgtaccgt ggggtacaaa ccaatgatat atcggtggat atgtgcaata caaaagaatt
    31681 tatccagatt tacaatacgc ttgatgttac taaaggcagc cgtattacag ccaatccgga
    31741 caccctgcct tctactaact ggtggaatga aatttctaac aatgccgtta tggaagaata
    31801 caatctttca gtctccggcg gaaccgaaaa catgacagct ctttttagtc ttggctattt
    31861 aaaccaggat ggtgtggttg aaaaaacaaa ctaccaacgt ttcaatgtca ggtcaaatac
    31921 ttcttacaat attggcaaac gtgtcaaaat tggtgaaaac atttctttgg ctgcaacccg
    31981 ctacagaggt aatgatggct atggttcgcc catgggggct atcaaccaaa gccctattag
    32041 ttatgtacgc gacacaactt ctggcttaac agcccaacaa attaaggcca aaaacattgg
    32101 atttgatggc tgggctcaac ctcttttcaa tactggcact ggtaaccctg ttgccggata
    32161 tattacgaca ataacaaaaa aggcacctac aggatggccg gaaacttatt tgccaatatc
    32221 gagctgctta aaggctttac ctacaacaat aattttggat ttgatatcaa tttttacgaa
    32281 gaagacgatt ttaaaccata ctattatatt acttctactc aaaacaactc tgttgttcag
    32341 gtgtaccgaa aattagaccg aaatttctca tggaattggc agcacgtact cgattataaa
    32401 acaacgttgt ttgacaaaca caatattgac ctgatggcag gccttgaagc taatgaatac
    32461 cttggcaaaa cactaagtgg caatgctgat tcattgttga aaaatggcgc tacacctgaa
    32521 tatcagtata ttgatgctac tctccgccaa acaggctcaa catactacta tgcaaagggc
    32581 ggatatagcc atcaaacagg ttacgcttat tttggccgct tgaattatga atttaacaat
    32641 ttattccttg cccagtttac ttatcgctac gatggctcta caaaatttgg cccaggtcac
    32701 aggtttggga gctttccggc attttccacc gggtttaagt tcagtgaact ggattttgta
    32761 aaaaacagcc ttcctttgct aagttttggt aaaatccgtt ttggatgggg aaaaaccgga
    32821 aatgatggta tcccaagtaa taaattttac tctttggttg gggcaagcgc cgaaaatggt
    32881 tatccattgg gaggcacagg tactccggga ggtattgctc tggccccggg gaactcccaa
    32941 ttgcactggg agtcaatcac aacatacaac tacggactcg atttgaattt ctttgacaac
    33001 aaactgtccg taacaaccga ttatttcgaa aaatatacca ccgggatgtt gcaatcttta
    33061 tcattgcctt tagttaccgg aagatacggt tttgacgggt ctgacggaaa atataccgac
    33121 catattggaa gtctttcaaa caaggggatt gaattagctt tggggtataa agacaatgtt
    33181 ggcgacctca aatatactgt tgatttcaat atcacaaaaa ttgtcagcaa ggtctatgat
    33241 ttaacagata caattacttt acctgactgg gaatcaaacc ctaaagctat cttgagaaat
    33301 ggagatgctc ccggagcttt ctggggttac aaaacagatg gtttgttccg tccaagcgat
    33361 gttgacacta ttattgaaaa cggtaaaaaa agacaagtat ggaaggctgg acttccgtat
    33421 aaaattgatg cccagggaag aagggtatat gcccaaaata cagcaaaacc aggcgatttg
    33481 aggtttgttg ataccaatgg cgacaccgta ctaaacgaca aagataaagt ggtgattggc
    33541 aaccctaatc ctgactttac ttttggtttt actattaatt tagagtataa agggttcgac
    33601 tttaactgct ttttccaagg cagctatggg aacgacattt tcaacacaag taaagcaagc
    33661 tggtacaacc ctaatgggct aaacaactgg gtaaaagatg cccttaatgc ttatcgcgac
    33721 cctgtttatg acaaaaatgg agtgatgata gatccgggaa ataccacctc taaccaattc
    33781 aaacttaaag gctctacctc tgataattac aggatgagcg attggtacgt tgaagatggc
    33841 tcttatatca ggctcaaatc aatacaaatt ggctatactt tgcctgaaaa cttcaccaaa
    33901 aaatacggga ttgaacgttt gagagtatat gtaggcggcc gcaaccttat tacctggacc
    33961 aatta
//
LOCUS       DAOSPF010000036        33669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1339_length_33669_cov_6.960255, whole genome shotgun sequence.
ACCESSION   DAOSPF010000036 DAOSPF010000000
VERSION     DAOSPF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1339_length_33669_cov_6.960255"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK252_08435"
     CDS             <1..302
                     /locus_tag="PK252_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14775.1"
                     /translation="LPYNNRLIPFIDMRQWFELPGEYDEKIKVIIVASQVGTLALLAD
                     RIVGEHQAVLKPLGKSFKKQQFLTSASQLGNGEIAFMLDTTKLLQTITSSILQSN"
     gene            314..778
                     /locus_tag="PK252_08440"
     CDS             314..778
                     /locus_tag="PK252_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT14776.1"
                     /translation="MDTYLSFIVHNELYAFNVIKVLEVLEKQRITHVPNTPDFIKGII
                     NFRGEVVPVFESRKKFNLQDRPEDESYVIIVLDLTKNEDIFHIGVIVDRVKDVITFDE
                     KEIKPVPAMSKDFNSDFLLGIYKLNNDFIMLLDVEKVFSLDEVRAMVEIAEA"
     gene            795..1400
                     /locus_tag="PK252_08445"
     CDS             795..1400
                     /locus_tag="PK252_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOT14777.1"
                     /translation="MAFTFFFRDDQILTQAVQKLVPMVMGRTNIHILNAGCAMGMETY
                     TFAIILAEHMGKFALRNVKIHAIDIDNENVDFGKTVTEAVYHKDHLERLPEGFLEKYF
                     TPIGDNVHYRVIEHLRALVNFQKYDLLSLKPLRSDYSMIICKNVLLHFNYEQRLDVLS
                     MYHDMLLPGGLLVMENTQSMPEELKGKYRKMLDYAQLHEKI"
     gene            1412..2017
                     /locus_tag="PK252_08450"
     CDS             1412..2017
                     /locus_tag="PK252_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT14778.1"
                     /translation="MNLTQNSKIYTSNAFLVLGEWNALDDVNTLIDVGCDKEIIAQIE
                     SINTGLGKRKIDQVILTHSHSDHTALLPKIIEMYNPHVYAFNKHIQGVQKQLTDGETI
                     KIGEKHFEIFHITAHSYDSICLYCEEDSVLFSGDTTFPIEFDNTELEEANDQILKRLC
                     SKKVNKLYPGHGNMIDFKTKKFQIKKKNTFQIHECNNNDDF"
     gene            2044..4830
                     /locus_tag="PK252_08455"
     CDS             2044..4830
                     /locus_tag="PK252_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOT14779.1"
                     /translation="MNWLQNMKIRTKLLSSFIIVSLLAGLIGYTGITRLHQVDDASTQ
                     LYEKMTVPLSYVVEVASYFQRVRVNVRDAVYSDSESDREKYFKRIDELKVLFFENAKP
                     IEETLLTNEGKVVFNNLITAFNNYIAEIENVKTFMKNNKKEEALALMKGKMKIANDEC
                     QKAVDKLQELKTDLAKKEADNNKAITNSATNFMITLILIVIALSVILGFIIANNIQNI
                     IKSVLKTTTELSEAAVEGKLSTRGDPEKINPEFRGIIIGLNNTLDAVIGPLNVAAEYI
                     DRISKGNVPAKITDTYHGDFNEIKNNLNACIDAINMLIIDANMLSTAAIEGKLATRAD
                     ASKHDGDFAKIVKGVNDTLDAVIGPLNVAAEYVDRIAKGNIPSKITDNYNGDFNEIKN
                     NLNLCIDNLNGLVSEMSHMSQQHDLGDIDVTMDISKFDNAYRNMADGVNKMVAGHITV
                     KKKAMACVNQFGEGNFEAELERFPGKKAFINDTIEGLRKNLKNVTGDVRILSSAAVEG
                     RLSTRADAGKYKGDWFEMVKGVNDTLDAVIGPLNVAAEYVERISKGDNPSVITDSYNG
                     DFNAIKNNLNTLIRANNEIIDKAKLVAKGDLTISLQKRSEHDELMAALDEMVKSNSKM
                     IGDFKVAIENIVNASQALQSVAIQISEGSTEQASSTEEVSSSMEQMVSNINQNTDNAR
                     QTEKIALQASTDISEGNKAVTITVDAMKKIADKISIIGEIAEKTDLLAINAAIEAARA
                     GEQGKGFAVVAAEVRKLAENSQAAAKEINELSKSSVKIADESGKLLTKIVPDIQKTAV
                     LVQEIAAASMEQNAGANQVNNAVMQLNAVTQKNAAAAEEMSSSAEELASQAEQLKEVI
                     SFFKTAHDTHGHYSSKKTQTQIKSTGGNGSEKREFNKSYVYNQRQEAFADNSFNLNGN
                     DLNFETNNDEFVKY"
     gene            complement(5100..5177)
                     /locus_tag="PK252_08460"
     tRNA            complement(5100..5177)
                     /locus_tag="PK252_08460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5140..5142),aa:Val,seq:tac)
     gene            complement(5303..5989)
                     /locus_tag="PK252_08465"
     CDS             complement(5303..5989)
                     /locus_tag="PK252_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT14780.1"
                     /translation="MYWIILILSIGFSWYIGWKLKKRFKEYSEITLAQNLSGKDVAEK
                     MLRDNGITDVKVISVEGELTDHYNPINKTVNLSPDVYYGRNVSAAAVAAHECGHAIQH
                     AHSYAWLTMRSKLVPLQNVSAQVINAVFIIMFIGSFFVHGLFPLALPIIIFCYFIFTV
                     FAFITLPVEMDASRRALVWLSSTNITNDYMHSKAEDALKWAARTYLVAALSSLSMLIY
                     YVMVYLSNRD"
     gene            6118..6513
                     /locus_tag="PK252_08470"
     CDS             6118..6513
                     /locus_tag="PK252_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14781.1"
                     /translation="MKIAIDFDGTIVEHAYPAIGKEIPFAFSTLKMLQQQGHLLILWT
                     FRSGDLLQEAVDFCQKKGITFYAVNASYPEEVYDPAASSRKIDADIFIDDRNIGGFPG
                     WGEIYQMLHPDDKNGVRIKKKKNFFSFIK"
     gene            6520..7605
                     /locus_tag="PK252_08475"
     CDS             6520..7605
                     /locus_tag="PK252_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOT14782.1"
                     /translation="MKGIFFYSFCIALITCQCQMQPKSSELNLNNDNNDAFSALQIIY
                     PHNGQIFTCGEETNLTFKKSADTIKIDSIKVYINGSEQQTIDINKLSAKIKLPENKTG
                     FIPIEYVVFIGGLKKQTNIVNVKLLSNIVPQKKQYKIVKTYPHSTEAYTQGLIFENDF
                     FYESDGEYGKSALRLVKPETGETLNNSALPNSYFAEGIAILNNEIYQLTWRERTCFVY
                     DKKSFAQKRTINYSINEGWGLATSSNKLLMTDGSNLLYTIEPSTFDVISKVEVYDNTG
                     PVDKLNELEYVNGLVYANVYMTDKIVVIDPASGKIVEYLDMNGLLKPSDKKPDTDVLN
                     GIAYNPNNGHFYVTGKNWPKLFEIKIY"
     gene            7814..11086
                     /locus_tag="PK252_08480"
     CDS             7814..11086
                     /locus_tag="PK252_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT14783.1"
                     /translation="MTGYNVYDFFGQADLNEMSVLNITQCNTLFQSLDIPIWAIDTKY
                     KLIAFNKAFSNYFKLKYRTEVYNGMNIEHFIKTDKLTDWNNLVSRSLKKQRPKAETQL
                     VFNKTTHYFEIVAQPIIENETVIGISFLSHDITDRKRTIGILSKAKAQLQALINNIPA
                     LIWLKDQNANFTVLNKSFYDFFQLNETEFAPESFDIPETHQSFFNYISNDKDIFAHGG
                     KIYEERSFKFGNEEKWFELHKTAIYNEKQEFVGTTGMANDITTRKTIENILLESEEKF
                     RQFAENTADSFILSNAGHILYINPAFEKIYGHKPEELTKAEIPLEWLHKDDRKAIASY
                     FSSEEFISTGKFNGEYRIVKPDNSIRWIWERRFPVMDEYGNIIRFISVASDITKQKLL
                     EFDLLKNQAQQQAILDNIPHQAWLKDITGKYISVNEAFAKHYNRPKNEIIGKTDSDIC
                     HPDLAELYAYNDYLVVKTKNQQQFDEFLDTNEGTFYTETIKTPIIDAEGEVIGITGIS
                     RDITYYKRLEQQLRLNDDRVKALLKHSSDSITVIDTESRITFDSTLFFKIANISSDDA
                     IGHSFTEFINPTDEGLVYYTIENAIKNPEIQQKIEYSCIKPDGQIIYFESIFSNHLKN
                     SLINGIVVNTRDITEKKKTEKIEQEYQDKVGFLDKTALDFLSLTSSDEIYEYIGNKIH
                     ELMPDAVILFSIYDETLDSLIIKSLTGINKFKTVVFDILGQDPINYPMHLNKLMKRKL
                     VRSANKLHELNGGLYNITSRKLDFMICKALEKLVSFNKAYGMGIVRSEKLLGSIIIIT
                     RYEQEIADPRIIETFTYQASIALLRRKLEKELVIAKEKAEESDRLKTAFLANMSHEIR
                     TPVNGIIGFSQLLLDPQLDQNRKQEFVDIIKQNANNLISIIDDILDISRIQEGQIKLK
                     KTFININLLLDEIFSFYLSPEWLEKNIVLDIEKSLDDEDAVMLIDPLKFKQIFNNLIT
                     NAFKFTREGKITIGYEADDEFFRFFVRDTGIGIAKENHDIIFQRFMQADGTLTRQFGG
                     SGLGLAISKGLVELMGGKIWVESEVNKGSEFSFTLPRLKEKKDHLKSLIQSAKDNPSL
                     LN"
     gene            11089..12222
                     /locus_tag="PK252_08485"
     CDS             11089..12222
                     /locus_tag="PK252_08485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT14784.1"
                     /translation="MRPDVAILGICFSLFVLYAIHIMAFTYGWFKAKPEIKSNLHHYT
                     FVSVVVAARNEEHNILHLIKSLWQQNYPKEYFEIIIVDDHSTDNTYTIVEEKKTFIKN
                     LKISRLYNNQSGKRNAIRHAISLSNGNLIITTDADCIVGNNWLKSIVYTYQNKNAKFI
                     IAPVLITDEKTFIQHYTSVEQLSLSACTIGAASLGHPVMCSSANIAFEKEFFEKIDTK
                     IPDNIRSGFDMFWLQEAIKKSASDISFVKSAEAAVYTKAPGSLKTFVSQHSRWASKNK
                     FLKNLWIISTALSVVLLNTLITILPIISCFYTKAMGVFLTLLFAKIIVDSPIMFAAMY
                     HYKKPRLLPWYIISSLLYPFVTSIVAIFGIFGRYQWKDRNIQQ"
     gene            12219..13358
                     /locus_tag="PK252_08490"
     CDS             12219..13358
                     /locus_tag="PK252_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14785.1"
                     /translation="MNENVNPRQSLWSKLYSNKLAMISMIFITLFCLLAILGYTIVND
                     PTPFSNRQCLELTAKKPGYQASFLLKPNNFETPKVSWFSKMIWGQPDTAQYILADSLE
                     FSGTTVIIFRKGNGKFFEKEHYRAKDLGLTNITDVNTFKKHVLQHLYITKHYLLGTDR
                     LGRDVLSRLIIGARVSLSVGLVSVIISLLIGVWLGAMAGYFGGRADRVIMWFVNVSWA
                     VPTLLLVIALTMVIGKGFWQIFIAIGLTMWIDVTRLVRGQIMGLREKEYIEACHAMGF
                     GHFRIITKHLLPGIIGPVIVLSAANFASAILIESGLSFLGVGIQPPVPSWGNMIKEHY
                     GYIILDKAYLAILPGLAIMLLTLAFTIIGNALRDATDTKAPIAPS"
     gene            complement(13364..13948)
                     /locus_tag="PK252_08495"
     CDS             complement(13364..13948)
                     /locus_tag="PK252_08495"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT14786.1"
                     /translation="MSKGAAKRFVDEAKRFINEDISISRFGSTMENIQKLQESYVRIS
                     EENKQLKHDNEGFRMVLTALSRENEYLQDNLRLLEEVLNRLPLIVSVRDVKKRSLLWY
                     NRNFHCMLGYRHKELQELINKEAIYHYHPEDYRKVREREKYVSSACNNSYSCNLRLKH
                     IDGRYVNMQSDFFVLKRNADGSHREVIEILSAIE"
     gene            14309..15382
                     /locus_tag="PK252_08500"
     CDS             14309..15382
                     /locus_tag="PK252_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14787.1"
                     /translation="MIKIKKILGLVILSMLAFSCNKEEGEGGKGNIKGIVYQVIVNAQ
                     TLDTITIAPAPDEDVYLIYGDDKEIDDKTVSSYDGSFEYKYLRDGNYQLLVYSQDTSN
                     YSKTKTPVIKKVHISNGGTAKPDTLFILKTVDYDKGAGIIEGIVNAEVIDNQNLNIIA
                     IQAAPDQNVYILNSSTNQVLDKTTTSDDGKFAFQNLVDGNYCILVYSQDTANAKNKKV
                     AIKQPVQVKNQNTVKLSSIHITKMLDIDEGSGSITGKIWLINYKSNGYTIKDIALAQE
                     YDVYLIYGNHLGFDMRTRTSYDGTFCFSNLINGKYTVFVYSEQEDIDGYLTGATQKAI
                     HKKTFEITNNNQSFYMTDTVKTF"
     gene            15404..15817
                     /locus_tag="PK252_08505"
     CDS             15404..15817
                     /locus_tag="PK252_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14788.1"
                     /translation="MKIQLITGLLLLSTVLSVSAQKKKKDIVSNKIKSITEFNEDFEK
                     NNGRSVKESFTAYDEFGNIIELIEYDDNGKEKKHIKYEYDDKDNCTKETYLNPKGQLE
                     KTIVFKYDDGLKIEKTTYLPNGKIKVRKKYIYQFQ"
     gene            15823..16575
                     /locus_tag="PK252_08510"
     CDS             15823..16575
                     /locus_tag="PK252_08510"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOT14789.1"
                     /translation="MGTIYHEIDTVLNTYKPISLSEMDSVKLMSRTDTKFIFNSAMIC
                     QILEMAKSYYKVLEINSTRQFQYITTYFDTPDLFMYNQHHNGKLNRYKVRQRRYDATG
                     NEFFEIKFKNNKGRTIKSRIENNANNELNAATDKFLKKKTPFECCQLKKTILNQFIRI
                     TLVNNNMTERATLDYNLCFSDFHKERPIPQLGIAEIKQDSNSGPSPLITIMRELKIQP
                     EGLSKYCLGVASLYDDVKINTFKPRLIKINNL"
     gene            16577..17263
                     /locus_tag="PK252_08515"
     CDS             16577..17263
                     /locus_tag="PK252_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOT14790.1"
                     /translation="MDTPTIETMKFLGIELFDKKDFFELLIRLSFNLLIVGIIVRLLY
                     YPITRRKDYLFTYILLSTIVFLICFMLSNVKLQMGFALGLFAVFGILRYRTNPMPIKE
                     MTYLFVVIGISIINSLTNKKVSYAEILFTNIAIVFVIYGLEKVWLLRHESCKLINYEK
                     IELIKPEKHNELMADLQQRTGLKINRIDIGKIDFLRDTARIMIYYYSDENRINLADEG
                     RVYTGDGDDD"
     gene            17223..17969
                     /locus_tag="PK252_08520"
     CDS             17223..17969
                     /locus_tag="PK252_08520"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOT14791.1"
                     /translation="MKAAYILEMAMMINKIKNILLCCFWGALSLTLAAQQDFNQWSTI
                     DFQYNFNKRNAVWVSNEMRLNENASQFSKYNFDAGYDRKIWEKLYAGVSFRYSRVNDK
                     FVYKNGYDYNLYAEYEFSYQRYSLDVKIRFQHTSQGIDLETIEEVENVWRNKADISYK
                     IPYTHLSPYIAFEYFSEITDKGLWPQKYRLFGGVKYNINKSSAISAYYGLQYSLRKPN
                     ENYILGLKYSLKLKAKKNKDKEEDTSENEE"
     gene            complement(17972..18895)
                     /gene="queG"
                     /locus_tag="PK252_08525"
     CDS             complement(17972..18895)
                     /gene="queG"
                     /locus_tag="PK252_08525"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOT14792.1"
                     /translation="MLIHDEIIQLAQKNGFDACGLAKAKVDANTQLVYKKWLSAGFHA
                     SMDWMETNVHLRFTPQLLVENASTVVVVLYSYHTKLDVFEKSPVRIAQYAWRRDYHKT
                     LKKKLKFFYNALQQKYPELQGRYFVDSAPVAERYFAEQAGLGWIGKSGMLINPVLGTN
                     CFIGTIIINEQADKYDNPVENRCGTCQRCIEACPTNAIIGNSLIDSNKCISYQTIEMK
                     INSEKPIHNSFSGFIYGCDLCQQVCPWNSKAVAFDERVAESAKKWGDLTAEQWQSLTE
                     PEYAKMFEGTPVKRASFEKLKSNIDKVLAQR"
     gene            complement(18898..19215)
                     /locus_tag="PK252_08530"
     CDS             complement(18898..19215)
                     /locus_tag="PK252_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOT14793.1"
                     /translation="MENPILMLETEIATVLRGIYDPEIPVNIFDLGLIYEINVDESRN
                     VEIVMTMTAPNCPMADELVQEVHEKIKAIPAVNELKVNLTFEPPWTKEMMTEEAMLEL
                     GLL"
     gene            complement(19249..19680)
                     /locus_tag="PK252_08535"
     CDS             complement(19249..19680)
                     /locus_tag="PK252_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOT14794.1"
                     /translation="MTLQETQDAIVEEFLQFDDWLDRYNYLIEISKELQPLDNKYKAN
                     EYLIKGCQSRVWLSGELIDGKVVFTADSDAIITKGIIALLIRVLSNREPDEIISAELE
                     FIDKIGLKENLSPTRANGLVSMIKQMRMFALAYKTKMEQNK"
     gene            complement(19715..20932)
                     /locus_tag="PK252_08540"
     CDS             complement(19715..20932)
                     /locus_tag="PK252_08540"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOT14795.1"
                     /translation="MFDVNSVRKDFPILGSTVHGKPLAYFDNGATTQKPQCVIDAVVE
                     CYTKTNSNIHRGVHYLSDQSTEMYENARKNIQQFINARYSQEIIFTSGTTGSINAIAF
                     SFGEKFVKAGDEILISAMEHHANIVPWQMLCERKNAKLKVIPVDENGELKLDAVDSLL
                     TEKTKILAITHVSNVLGTVNPIKAIIDKAHKKGIPVLIDGAQSIQHVKIDVQELDCEF
                     FVFSGHKIYAPTGIGILYGKEEWLNQMPPYQGGGDMVDCVTFEKTYYNSLPYKFEAGT
                     TNYVDAVGLSVAIDYIKKLGFDNIAGYEKDLLEYATNKLQTIEGLKIYGTAADKGPVI
                     SFLLENIHPYDTGMILDKLGVAVRTGTHCAQPIMDFYEIEGTVRASMAFYNTFEEIDR
                     LYEGLLRVKTMFG"
     gene            complement(20959..22302)
                     /gene="sufD"
                     /locus_tag="PK252_08545"
     CDS             complement(20959..22302)
                     /gene="sufD"
                     /locus_tag="PK252_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOT14796.1"
                     /translation="METITTVERLIKTYSGIYHQNKGVFSDALSNHRQEAVSVFESMG
                     VPNKSDERYKYLDLTPWFDHTYGYTIQPEKLKLDSEQVFQCDVPRFDMHEIITINGFF
                     HSHTKPHELPLGVWIGSLAKALQEIPEKVIPYLDVDVNLADSLVSLNKAVFRDGIFIY
                     LPKNCNFHSSIQIINLQLAQDDMFVSPRNLIVLEGNSSVNLVICDHTLSQNKFLSNQL
                     TEIVVKEGAQLDVTNMQNIHNNAGAINHTNIMQKANSRVNYTVLSLHGGMIRNNIAAK
                     LDGVGADNSTNGLYMTDGSQIIDFNVLIDHAKPSCTSNQLIKGILDDTSVGSFNGKIM
                     VARDAQKTAAFQKCNNLLLKDTAKVNTRPQLEIYADDVKCSHGATVGQIDPDALFYLR
                     ARGISKDEARMLLMFAFAHEIVQNIKFENLRFRIDEIVNKRLRKELTRCHNCQAL"
     gene            complement(22334..23080)
                     /gene="sufC"
                     /locus_tag="PK252_08550"
     CDS             complement(22334..23080)
                     /gene="sufC"
                     /locus_tag="PK252_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOT14797.1"
                     /translation="MLHIKDLRAKIGEKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     AVLTGRENVEVTHGEVWFKGKNLLELSPEDRAREGLFLSFQYPVEIPGVSMVNFLRAS
                     VNEVRKYNNLPALSSADFLKFMREKQQLVEMDGKLTGRSVNEGFSGGEKKKNEIFQMA
                     MLNPALSILDETDSGLDIDALRIVSNGVNKLKTADNATIVITHYQRLLDYIVPDIIHV
                     LYDGRIVKTAGKELALELEEKGYDWIKKTV"
     gene            complement(23229..23513)
                     /locus_tag="PK252_08555"
     CDS             complement(23229..23513)
                     /locus_tag="PK252_08555"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT14798.1"
                     /translation="MLPAKTYRDLIVWQKAHEYVLLVYKVSSAFPKEEVYGLTSQYRR
                     ASVSVAANIAEGFRKKTNTEKLRYYNISQGSLDECSYYSLLVFDLGYIKC"
     gene            complement(23550..24998)
                     /gene="sufB"
                     /locus_tag="PK252_08560"
     CDS             complement(23550..24998)
                     /gene="sufB"
                     /locus_tag="PK252_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOT14799.1"
                     /translation="MSDNSQDIIQDVTGGDYKYGFYTNIDIDTIPKGLNEDIIRLISK
                     KKEEPEFMLEFRLKAFRHWQKMKMPDWAHLQIPEINYQDIIYYAAPRQQAKYSSMDEV
                     DPELLETFNKLGIPLSEQAHLAGVAVDAVMDSISVKTTFREALAEKGIIFCSMSEAIK
                     EHPDLVKKYLGSVVPFSDNYFAALNAAVFSDGSFCYIPKGVRCPMELSTYFRINAANT
                     GQFERTLLIADDDAYVSYLEGCTAPMRDENQLHAAVVELVAMENAEIKYSTVQNWYPG
                     DKNGKGGIYNFVTKRGICKGNNSKISWTQVETGSAITWKYPSCILRGDNTVGEFYSVA
                     VTNNYQQADTGTKMIHIGKNSRSRIVSKGISAGKSNNSYRGLVKVLKGADNARNFSQC
                     DSLLLGDKCGAHTFPYLEVENPNAIVEHEATTSKIGEDQIFYCNQRGISTEDAVGLIV
                     NGYAKEVLNKLPMEFAVEAQKLLQISLEGSVG"
     gene            25294..26082
                     /locus_tag="PK252_08565"
     CDS             25294..26082
                     /locus_tag="PK252_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT14800.1"
                     /translation="MQTNENAAFTKTAINLDEIVFANRNHAYGAYNMRKEYKKRLLIG
                     FIATFLIFSASIGISMYKTYTNIIVPKPEIKHDVTFAPDKLDNPLPPPPAPAPIMPTI
                     ERFTAPIIVDTTDENDLTLISMDEATALTTNDTEDLTYVPENTVNTYTPEIDEPEIGR
                     WDVTEQATFEGGDLKTFHAWLQMNVTYPQEAIEARLSGRITLFFSVNTKGQVCDVKVI
                     KGIHPVLDQEALRTILNSPAWKPAKFNGKPVKQNFTVPLIFRIE"
     gene            26394..26729
                     /locus_tag="PK252_08570"
     CDS             26394..26729
                     /locus_tag="PK252_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT14801.1"
                     /translation="MPYKEKKIEKVYYTIGEVAEMFGVNTSLIRFWEKEFDVIKPKKN
                     KKGNRLFTKKDIDNFHTIYHLVKERGMTLKGAQKKMKENKEDTENNFEIIKTLKAIRE
                     MLVEIRENM"
     gene            26732..27823
                     /locus_tag="PK252_08575"
     CDS             26732..27823
                     /locus_tag="PK252_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT14802.1"
                     /translation="MQIKEILTTLEDFAPLAFQEPYDNSGLCIGNPNKEVKALLLTID
                     ITEDVIDEAIKLGCNLIISHHPLIFSGIKSLTGQNCIERCIIYAIKNDIAIYSAHTNL
                     DIAPMGVSYMMAQKLGLKNIKILSPLENKLLKLVSFVPFEHAEKVREAIFEAGAGHIG
                     NYDRCSYNLEGNGTFRGNENSNPFVGISGQDHTEPEIRIETILPAHIKNKVLKALLNA
                     HPYEEPAYDIYPLLNTFEKAGLGAIGKIEPVEEKVFLKKIKEVFNVTIVRHTPLQNKK
                     IETVALCGGSGSNLLKNAIKEQADIFISSDFKYHQFFDHEKKILIADIGHFESEQFTK
                     EIFYQIIKKKFANFAVHFSSINTNPINYY"
     gene            27828..28580
                     /locus_tag="PK252_08580"
     CDS             27828..28580
                     /locus_tag="PK252_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT14803.1"
                     /translation="MAHTESEISIEEKLRALYELQRVDSEIDKIKILRGELPLEVQDL
                     EDEIAGLDTRLQNLEEEVKNLDVAISTKKNEIVNANALIKRYKEQQENVRNNREFDSL
                     SKEIEFQTLEIELCEKRIREFTAQIKNKKEIIESSSATLKERNNDLKNKKKELEEIIA
                     DTQKEEEALLKKSKEIEKLIEARLMNAYKRIRSNARNGLAVVSVERDACGGCFNKIPP
                     QRQMEIKAHKKIIVCEYCGRILVDKNYFESQN"
     gene            28889..29824
                     /gene="mdh"
                     /locus_tag="PK252_08585"
     CDS             28889..29824
                     /gene="mdh"
                     /locus_tag="PK252_08585"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOT14804.1"
                     /translation="MKVTVVGAGNVGATVAQCIAEKELVNEIILLDIKEGLSEGKALD
                     LWQTAPVNYYDSRIKGVTNDYKSTENSEVIVITSGVPRKPGMSRDDLISINANIVKSV
                     AENAIKYSPNAKIIVVSNPLDVMTYVAYLAAKTSPEKVLGMAGILDTARYRAFLADEL
                     KCNPKDIQALLLGGHGDTMVPLPRYTTVSGIPVTQLIEKEKLDAIIKRTQNGGGEIVS
                     LLGTSAWYAPGASAAQMVEAIIRNQNRIFPCCVKLDGQYGYKNVFVGVPVKVGKNGVE
                     QIIEIELNAEEKASFDNSVSAVKTLMDVLDNMKLF"
     gene            complement(29878..29964)
                     /locus_tag="PK252_08590"
     tRNA            complement(29878..29964)
                     /locus_tag="PK252_08590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29928..29930),aa:Ser,seq:cga)
     gene            complement(30066..32255)
                     /locus_tag="PK252_08595"
     CDS             complement(30066..32255)
                     /locus_tag="PK252_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOT14805.1"
                     /translation="MAHLRFKALEQVFNRKPVEVKAPATKASEYFGMYVFDKPKMKKY
                     LSKEAYQAVELAIDKGICIDRKMADQVAAGMKAWALEMGATHYTHWFHPLTEGTAEKH
                     DAFAEPAGNGSVIESFTGKLLVQQEPDASSFPSGGIRSTFEARGYTAWDLSSPAFILD
                     QTLCIPTIFLSYTGEMLDNKAPLLKALHAVDQAAVAVCQYFDKDVQKVYSFLGWEQEY
                     FLIDQSLYLARPDLMLTERTLMGHDSAKNQQLEDQYFGSIPERVLAFMQELEYEAWKL
                     GIPAKTRHNEVAPNQFEIAPVYEECNLAVDHNLLIMSIMRRVAKKHHFQVLLHEKPFK
                     GVNGSGKHNNWSLGTDTGVNLLSPSKNPKGNLLFLTFCVNVLKAVHNNDDILKASIVS
                     AGNAHRLGANEAPPAIVSAFLGEQISKMLDEIEQKVTGSKLSADDKTALKLDIGRIPE
                     ILLDNTDRNRTSPFAFTGNRFEFRAVGSSANCAPAMTALNAAVAQQLTIFKKDVDALI
                     KKGIKKDDAILQILARYISESKAIRFDGNGYSEEWKKEAKRRGLNTEGAVPVLNKVWA
                     SPKAARLFGETKVYSHKELEARAEIRQEIYMKKIQIESRVLGDLAMNHIVPVAVDYQT
                     SLIENIKAMKDIFSEKEFKELSAKRIELVKEVNGHISTIKSKVSEMIEARKVANKIED
                     VNEMAMAYYEKVVPFFEEIRYHIDKLELIVDNEKWPLPKYRELLFIR"
     gene            32806..33579
                     /locus_tag="PK252_08600"
     CDS             32806..33579
                     /locus_tag="PK252_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT14806.1"
                     /translation="MKILIIGAGNMGSWLVEALCLEHEVAVFDTDKKKLKFLFNSHRL
                     TQLSEISNFQPELVINSVSLQYTFAAFESILPYLPQTCIISDIASVKNGLFDFYKKSG
                     FRFASIHPMFGPTFANIKELAQQNAIIIKESDPEAKEFFRNFFQSLHLNIFEYTFEEH
                     DQTIAYSLSVPFATTLAFASCIKKQEAPGTTFKKHMVIAKGLLSENDYLISEVLFSPY
                     SLKQIENIKTKLEMLEEIIKNKDTKAMHHFLKNVRKNIE"
BASE COUNT        11502 a         6682 c         5535 g         9950 t
ORIGIN      
        1 cactgccata taataatcga ctcataccat ttattgatat gcgtcaatgg tttgagttac
       61 ctggtgaata tgatgaaaaa attaaagtaa ttattgttgc atcacaggta ggcacattgg
      121 cattattagc tgatagaatt gttggagaac accaggctgt attaaaacct ctcggtaaat
      181 cattcaaaaa acaacagttc ttaacctctg caagtcaatt aggcaatgga gagattgctt
      241 tcatgcttga tacaactaaa cttctacaaa caataaccag tagtattctt caatcaaatt
      301 aaatcataaa actatggata cttatttatc atttatcgtt cataacgagc tttatgcttt
      361 taatgtcata aaagtattgg aagtacttga aaaacaaaga atcacacatg taccaaacac
      421 tcctgatttt atcaaaggaa ttatcaattt cagaggcgaa gtagtgccgg tttttgaatc
      481 gagaaaaaaa ttcaacttgc aggataggcc tgaggacgag tcctatgtaa tcattgtcct
      541 tgatttaacc aaaaacgaag acatattcca tattggagtt attgttgaca gggttaaaga
      601 cgtaatcact tttgacgaaa aggagataaa acctgtacca gcaatgagca aagatttcaa
      661 ctctgacttc ctgctcggaa tttataaatt aaacaatgac ttcattatgc tccttgatgt
      721 tgagaaggta ttttcccttg atgaggttag ggcaatggtt gaaatagctg aagcttaaaa
      781 catcatcatc gattatggca tttacattct tttttcgaga cgatcaaata cttacacaag
      841 cagttcaaaa actggtacca atggtaatgg gacgaacaaa catacatatt ctaaatgctg
      901 gatgcgcaat gggtatggaa acatacacat ttgcaattat tctggcagag catatgggta
      961 agttcgcttt aagaaatgta aaaattcatg ccatcgatat tgataacgaa aatgttgatt
     1021 ttgggaaaac tgtcaccgaa gctgtttacc ataaagacca tttagaaaga ctgcccgaag
     1081 ggtttcttga aaaatatttc acccctatag gagataatgt tcactacaga gtaattgagc
     1141 atcttagagc attagtaaat ttccagaaat acgatttact atcattaaag ccgctgcgct
     1201 cagattatag catgataata tgcaaaaacg tattacttca ttttaattat gaacaaaggc
     1261 tagacgtttt atccatgtat catgacatgc ttctccccgg cggtttgctg gtaatggaaa
     1321 acacacaatc aatgccagaa gaactaaaag gtaaatacag gaaaatgctt gattatgctc
     1381 agttacatga aaaaatataa aaatgaaaat catgaacctc actcaaaaca gtaaaattta
     1441 cacatctaat gccttccttg tacttggtga atggaatgct ttggacgatg taaatacgct
     1501 tattgatgtt ggttgcgaca aagagatcat cgctcagatt gaaagcataa atactggttt
     1561 ggggaaaaga aaaattgatc aggtaatctt aacacacagc cattctgacc atacggcact
     1621 tcttccaaaa attattgaga tgtacaatcc ccacgtttat gcctttaaca agcatataca
     1681 aggagtacaa aaacaattga ctgatggtga aacgattaaa attggagaaa agcactttga
     1741 aatatttcat attacagctc acagctatga ttctatttgt ctttattgcg aagaagattc
     1801 agttttgttt tccggagaca caacatttcc aatagaattt gacaataccg aattagaaga
     1861 agccaatgac caaatcttaa aaaggctttg cagcaaaaaa gttaacaagc tttatcctgg
     1921 gcatggaaac atgatagatt tcaaaactaa gaaattccaa attaagaaaa aaaacacttt
     1981 tcagattcac gaatgcaaca acaatgatga cttttaataa ttaataatta aaaaataaga
     2041 cttatgaatt ggttacaaaa catgaaaatt aggacaaagt tacttagtag ctttattatt
     2101 gtttccttat tagcaggttt aataggctat acgggcatta caagactgca tcaggtggat
     2161 gatgctagta cacaactata tgaaaaaatg acagttcctc tgagctatgt tgttgaagtt
     2221 gcttcctatt ttcaaagagt aagggtaaat gtaagagacg cagtttattc agattcagag
     2281 tctgacagag agaaatattt caaaagaatt gatgaactta aagttttgtt cttcgaaaat
     2341 gcaaaaccaa ttgaagaaac tttacttacc aatgaaggca aagtcgtgtt caataacttg
     2401 ataacagcat ttaataatta tattgctgag attgaaaacg ttaaaacctt tatgaaaaat
     2461 aataaaaaag aagaagcttt agcattgatg aaaggtaaaa tgaaaattgc aaatgatgaa
     2521 tgccaaaaag ccgttgacaa gcttcaggaa ctaaaaactg acttagctaa aaaagaggcg
     2581 gataataaca aagcaataac aaatagtgca acaaatttta tgattacttt aattttaata
     2641 gtaattgcat tatcagttat tttgggcttt atcattgcaa acaatattca aaacataatt
     2701 aaatcagttt taaaaacaac cacagagctt tctgaggcag cagtagaagg caaactttct
     2761 acacgtggcg atcctgaaaa aattaatccg gaatttcgag gtattataat tggactcaac
     2821 aacaccttag acgcagtaat aggcccgtta aatgtagctg ctgaatatat tgacagaata
     2881 tcaaaaggta atgttccggc aaaaatcaca gatacctatc atggtgattt caacgaaatt
     2941 aaaaacaatc tcaacgcctg tattgacgct attaacatgc tcattatcga cgctaatatg
     3001 ttatcaaccg ctgctattga aggtaaatta gccactcgtg cagatgcttc aaaacatgat
     3061 ggagactttg ctaaaatagt aaaaggcgta aatgacacat tagatgccgt tatcgggcct
     3121 ctcaatgtag ctgccgaata tgtcgaccgt attgctaagg gcaatatacc ttctaagatt
     3181 acagacaatt acaatggcga cttcaacgaa attaaaaaca acctgaacct ttgtatcgac
     3241 aatctaaatg gtttggtaag tgaaatgagc catatgtcgc aacaacatga tctgggcgac
     3301 atagatgtaa ccatggacat ttcaaagttt gacaatgcat acagaaatat ggctgatggt
     3361 gtaaacaaaa tggtagcagg gcacattacc gttaagaaaa aagctatggc ctgtgtaaac
     3421 caatttggag aaggaaattt tgaagctgaa cttgaaagat tcccaggaaa gaaagccttt
     3481 ataaatgaca ctattgaagg tctccgcaaa aacctaaaga atgttacagg agatgtccga
     3541 attctctcta gcgcagctgt agaaggccgt ttatcgacca gagcagatgc tggcaaatat
     3601 aaaggtgatt ggttcgaaat ggttaaaggc gtaaacgaca cacttgatgc agttattggc
     3661 cctcttaatg tggcagccga atacgtagaa cgcatttcaa aaggagataa tccttcagta
     3721 ataacagaca gttacaatgg agatttcaac gcaattaaaa acaatctaaa cacccttatt
     3781 cgagccaata atgaaatcat tgacaaagca aagctggtag caaaaggaga tttaacaatt
     3841 agtttgcaaa aaaggtctga acacgatgaa ttaatggcag ctttggatga aatggtaaaa
     3901 tccaactcga agatgatagg cgactttaaa gtagctatcg aaaacatagt aaatgcaagc
     3961 caggcgcttc aatctgtagc cattcaaatt tctgaaggca gcaccgaaca ggcctcaagc
     4021 accgaagaag tatcttcaag catggaacag atggtttcaa acatcaacca aaatacagac
     4081 aacgcaagac aaacagaaaa aattgcactc caggcttcaa cagacattag cgaaggcaac
     4141 aaagccgtaa ccattaccgt ggacgctatg aagaaaattg ctgacaaaat aagcattatt
     4201 ggagaaatag ctgaaaagac agaccttctc gccatcaatg cagcaattga agcagcccgt
     4261 gcaggcgaac aagggaaagg ctttgccgta gtagctgcag aagtccgcaa acttgcagaa
     4321 aacagtcagg cagcagccaa agaaatcaac gaactctcca aatcaagtgt aaaaatagca
     4381 gatgaatctg gcaaactttt aacaaaaatt gtacccgaca tccaaaaaac agcagtcctg
     4441 gtgcaagaaa tagctgctgc cagtatggaa caaaatgccg gcgctaacca agtcaacaat
     4501 gccgttatgc agctcaatgc cgtaacccaa aaaaatgctg cagctgcaga agaaatgtct
     4561 tcgagcgctg aagagcttgc cagccaagcc gaacaactca aagaggtcat ctctttcttt
     4621 aaaacagcac atgacactca tgggcattac agctccaaaa aaacacaaac ccaaatcaag
     4681 agtacaggtg gcaatggcag cgaaaaaaga gaattcaaca aaagctatgt ttacaatcaa
     4741 cgccaagaag cttttgctga caattccttc aatcttaatg gcaacgattt gaactttgaa
     4801 accaacaatg atgaatttgt taaatactaa gcctaaacct aaaccctaaa ccctgaaggc
     4861 tgaaggctgt gtaaaaaagc tataaaacgc caaaaacaca gccttttttt agttaatgac
     4921 aacataatta tcccaaatta atcattagga agttttttaa agccgaacta tatggttaat
     4981 ttgggtttaa ttgtttaaca aaactatatt tacatgaggc taaaaaagtt tgtgtgaaaa
     5041 tggtgcgcaa ataacatgaa aaaaggcctc cggaaataat cctgaaagcc ttgtcttttt
     5101 ggtgggagat acaggggtcg aacctgtgac cccctgcttg taaggcaggt gctctgaacc
     5161 aactgagcta atctcccttt tgctgttaaa gcgctgcaaa ggtaaaccag ttttataaat
     5221 ctgcaaatct ttttttaaaa attttacaaa aaatgcggaa tctttataaa attccgcatt
     5281 tcaacaatat gtacaacttt tattaatccc tattgcttaa gtacaccatc acatagtaaa
     5341 tcaacataga caatgatgac aatgcagcaa ctaaatatgt ccgggctgcc catttcaaag
     5401 catcttcggc tttactatgc atgtaatcat tggtaatatt ggtacttgac aaccatacca
     5461 atgcccttct gctggcatcc atttctacag gcaaagtaat aaatgcaaat acagtaaaaa
     5521 taaaataaca aaagataata attggcaatg ctaaaggaaa taaaccatgc acaaaaaatg
     5581 aaccaataaa cataataata aagacagcat tgataacctg agcacttaca ttctgcaacg
     5641 gaacgagctt agagcgcatt gtaagccagg cataagaatg cgcatgttga atagcatgcc
     5701 cacattcatg agctgcaaca gccgccgccg aaacattacg tccataataa acatccgggc
     5761 taagatttac cgttttgtta atagggttgt aatggtcggt aagctcacct tcaaccgaaa
     5821 taactttcac gtctgttatt ccgttatcac gcagcatctt ttcggccacg tctttaccac
     5881 tcaggttttg tgcaagagtt atctcagagt attccttaaa tcttttcttt agcttccatc
     5941 caatatacca gctaaaccca atcgagagaa ttaaaattat ccaatacata gttttatttt
     6001 ttttcaaaat taacaacaat ttctttgcca agtttattca cgtgtaattt tggcagcttt
     6061 tttaacattg gttaaccaaa acttttgttt acatttgctg ctcacacaaa gctttttatg
     6121 aaaattgcca ttgattttga cgggactatt gttgaacatg cctaccctgc tatcggcaaa
     6181 gaaatacctt ttgcctttag cacactcaaa atgcttcagc aacaaggcca cctgcttatt
     6241 ttgtggactt tcaggagtgg ggacctgcta caggaagctg ttgatttttg tcaaaaaaaa
     6301 ggcataacat tttatgccgt caatgccagc tacccggaag aagtgtatga ccctgctgcc
     6361 tcaagccgca aaatagatgc cgacatcttt attgacgacc gaaatattgg aggtttcccg
     6421 ggatggggcg aaatatatca aatgctccac cctgacgaca aaaacggggt tagaatcaaa
     6481 aagaaaaaaa attttttcag ctttattaaa taattcatca tgaagggaat atttttttac
     6541 agcttttgca tagcattgat tacttgtcaa tgccagatgc agccaaaatc gtcagaactc
     6601 aatctaaata atgataataa tgatgctttt tcagcccttc aaatcatata tccacataac
     6661 ggccaaatat tcacctgcgg agaagaaact aacctgacgt tcaaaaaaag tgcagacact
     6721 ataaaaattg attctatcaa agtatatatc aatggtagtg aacaacaaac aattgatatc
     6781 aacaaattat ctgccaaaat taagctcccc gaaaataaaa caggatttat acccatcgaa
     6841 tatgttgtat ttattggtgg cttaaaaaaa caaaccaaca ttgtcaatgt taaactgctc
     6901 tccaacattg tcccccaaaa aaagcaatat aaaattgtaa aaacatatcc tcactctacc
     6961 gaagcttaca cacaaggttt aattttcgaa aatgattttt tctacgaaag tgacggtgag
     7021 tatggcaaat ctgccctgcg ccttgtaaaa cctgaaaccg gggaaacttt aaacaacagc
     7081 gctttaccca actcatattt tgcagaaggt atagccatcc tcaataacga aatataccag
     7141 cttacctggc gcgaaagaac ttgctttgtc tatgacaaaa agtcttttgc tcaaaagcgt
     7201 acaataaact acagcataaa tgaaggttgg gggcttgcaa cttcaagtaa caaactgcta
     7261 atgaccgatg gcagcaacct tttatacact attgaacctt caacatttga tgtaataagc
     7321 aaagttgaag tttatgacaa cacaggccct gtagataaac taaatgaact tgaatatgta
     7381 aatgggcttg tttatgccaa cgtatatatg acagacaaaa tagtagttat agaccctgca
     7441 agtggcaaaa ttgttgaata tcttgacatg aatggacttt taaaaccatc tgacaaaaag
     7501 cccgacaccg atgtattgaa cggcatcgca tataacccca ataatggtca tttttatgtt
     7561 acaggaaaaa actggcccaa gcttttcgaa attaaaattt attaagccgt ctttctatac
     7621 actcttttaa ccttcacaat aaattacgag caaaattggc aatactacca gttggttcct
     7681 caatagttga caaaacctta atttccgttt atcacctaaa aaagtgtttt cataaaaaaa
     7741 actttggaca tcagtgatat tttttttatg ttgcagtcat aaaaatgacg taaaaaaaag
     7801 aaggattgac ggcatgacag gttataacgt ctatgatttt tttggacagg cagatttaaa
     7861 tgaaatgtca gtattaaaca tcactcagtg taatacattg tttcaaagcc ttgatatccc
     7921 aatctgggct atcgatacaa aatataagct tattgccttc aataaagcct tctctaacta
     7981 ttttaagcta aaatatagga ccgaagttta taatggaatg aatattgaac atttcattaa
     8041 aaccgacaaa cttacagatt ggaataattt agtttcacga agtttaaaga aacaaagacc
     8101 aaaagccgaa acccagcttg tttttaataa aacaacccat tattttgaaa ttgtagccca
     8161 acccattatt gaaaatgaaa cagtcattgg tatttccttt ttatcacatg atattaccga
     8221 cagaaaaagg accattggta ttttaagtaa agccaaagcc cagctgcaag cccttataaa
     8281 taacattccg gcgctaatat ggcttaaaga ccaaaatgcc aactttacag tgttgaacaa
     8341 aagcttttac gacttttttc aactcaatga aactgaattt gctccggaat ctttcgacat
     8401 ccccgaaacc caccaatctt tcttcaacta tatctcaaac gacaaagaca tctttgccca
     8461 tggcggtaaa atatacgaag aacgctcttt caagtttggc aacgaagaaa aatggtttga
     8521 gctccataaa acagccattt acaatgaaaa acaagaattt gtcgggacta caggaatggc
     8581 caacgatatt actacccgca aaaccattga aaacattctg ttagaaagtg aagaaaaatt
     8641 caggcaattt gccgagaaca ctgccgactc tttcattcta agcaatgcag gccatattct
     8701 ttacataaac ccggcatttg aaaaaatata cgggcataaa cccgaagagt taacaaaagc
     8761 agaaattcct ttagaatggc tccataaaga tgaccgcaag gccatagcat catacttttc
     8821 aagcgaagaa tttattagta ccggcaaatt caacggcgaa tacagaattg tcaaacccga
     8881 caacagcatc aggtggatat gggaaagacg cttccccgtc atggatgaat atggcaatat
     8941 tatccgcttt atctctgttg cttctgacat caccaaacaa aagttgctgg aatttgattt
     9001 gctcaaaaac caggcccagc aacaagctat cttggacaac atccctcatc aggcgtggtt
     9061 aaaagatatt acaggaaaat atatctcggt aaacgaagcc tttgccaaac actacaatcg
     9121 ccctaaaaat gaaattattg gcaaaaccga ctctgatata tgtcatccag accttgccga
     9181 attgtatgct tacaatgatt atcttgttgt taaaactaag aaccagcagc aatttgacga
     9241 atttctggac actaacgaag gtacttttta caccgaaaca ataaaaacgc ccatcattga
     9301 tgccgaaggc gaagttattg gcattactgg tatctcaaga gacatcacct attacaaacg
     9361 ccttgaacaa caactcaggc tgaatgacga cagagttaaa gccttattaa aacattcttc
     9421 cgactccatt actgttatag acaccgaaag ccgcatcaca tttgacagta ccctgttttt
     9481 taaaatagcc aatattagtt ctgacgatgc tattggtcat agttttaccg aatttattaa
     9541 cccaacagac gaaggacttg tttattatac catagaaaat gccattaaaa accctgaaat
     9601 acagcaaaaa attgaatata gctgtatcaa acccgatggt cagattattt acttcgaaag
     9661 tattttttct aaccatctaa aaaacagcct aatcaacggg attgttgtca atacccgcga
     9721 cattaccgag aaaaagaaaa cagaaaaaat tgaacaagaa tatcaagaca aagttgggtt
     9781 tcttgacaaa acagcactcg attttctttc gctcacctca agtgacgaaa tttacgaata
     9841 cataggaaac aaaatccacg aactgatgcc cgatgctgtg atacttttca gtatctacga
     9901 cgaaacactg gactcattga ttatcaagag cctcacaggc atcaacaaat ttaagactgt
     9961 agtttttgac atcctgggac aagaccctat aaactaccct atgcatctta ataagcttat
    10021 gaagcgcaag ctggtacgca gtgccaacaa actgcacgaa ctcaacggag gtctttacaa
    10081 tattaccagc cgcaagctcg attttatgat ttgcaaagcc ttagaaaaac ttgtatcttt
    10141 caacaaagct tatgggatgg gcattgtcag gtctgaaaaa cttcttggtt caatcattat
    10201 tatcactcgc tacgaacagg aaatagcaga cccgcgcatt atagaaacat ttacatacca
    10261 ggcttctatt gctttattgc gccgcaaact cgaaaaagag ctcgttatag ctaaagaaaa
    10321 agctgaagaa agcgataggc ttaaaactgc ctttttggcc aatatgagcc acgaaatacg
    10381 caccccggta aatgggatca ttgggttctc tcaattactg cttgatccgc aacttgacca
    10441 aaaccgaaaa caagaatttg ttgatattat caagcaaaat gccaacaacc ttatttcaat
    10501 tattgacgac attcttgata tttcacgtat ccaggaaggc cagataaaac tcaagaaaac
    10561 tttcatcaat attaatttat tgcttgatga aatattttca ttttatctct cccccgaatg
    10621 gctggaaaaa aacattgtat tggacattga aaaatcactc gatgatgaag atgcagtgat
    10681 gcttatcgac ccgttgaaat ttaaacaaat attcaacaat ctcattacca atgctttcaa
    10741 atttacccgt gaaggcaaaa tcaccattgg ctatgaagcc gatgacgaat ttttcaggtt
    10801 ttttgtacgt gacacaggta ttggaattgc aaaagaaaac cacgacatca tattccaacg
    10861 ctttatgcaa gctgacggca cactaacaag acaattcgga ggttcaggcc ttggacttgc
    10921 catatctaaa ggacttgtcg aattgatggg aggaaaaata tgggtagaat ctgaagttaa
    10981 caaaggttcc gaattttcat ttaccttacc caggctcaaa gaaaagaaag accatctcaa
    11041 gtcgcttatc cagtcagcga aagacaaccc ttccttgctc aattaacaat gcgacctgat
    11101 gttgccatct tgggtatttg cttttcgttg tttgtcttat atgccattca catcatggca
    11161 ttcacttacg gttggtttaa agctaagccg gaaataaaaa gtaatttgca tcactatacg
    11221 ttcgtttctg ttgttgttgc tgcccgaaat gaagaacaca atattctgca ccttataaaa
    11281 agcctttggc aacaaaatta tccaaaagaa tattttgaaa ttattatcgt tgacgaccat
    11341 tcaaccgaca acacttatac tattgttgaa gagaaaaaaa catttatcaa aaacttaaaa
    11401 attagcagac tctacaacaa tcaatcaggt aaacgcaacg caatcaggca tgccatttcg
    11461 ttatccaacg ggaatttgat tatcacaact gatgccgatt gcattgttgg caacaactgg
    11521 ttaaaatcta ttgtctatac ttatcaaaac aaaaatgcta aatttattat tgcccctgtg
    11581 ttgataaccg atgaaaaaac atttatccag cattacacct ctgtagaaca gctcagtctg
    11641 tcggcatgca ctattggtgc cgccagctta ggccatcctg taatgtgcag tagtgccaac
    11701 atagcttttg aaaaggaatt ttttgagaaa atagatacta aaattcccga caacatccgt
    11761 tctggcttcg acatgttttg gcttcaagaa gccataaaaa agtccgcttc agatatttct
    11821 tttgtcaaat cagcagaggc ggcagtatat acaaaagccc caggttcact aaaaactttc
    11881 gttagtcaac attcaagatg ggcttcaaaa aacaaatttt taaaaaatct ctggattatt
    11941 tcaacagctt tatctgtagt tcttttaaac acattaataa ctatattgcc tattatttct
    12001 tgtttttata ctaaggcaat gggggtattc cttacgctgc tttttgccaa aattattgtt
    12061 gacagcccta tcatgtttgc tgccatgtat cactacaaaa aaccaagact gttgccgtgg
    12121 tacattatct catcactatt gtatcctttt gtaacctcaa tagtagcaat ttttggtatc
    12181 tttggccggt atcaatggaa agacagaaac atacagcaat gaatgaaaac gtgaatccca
    12241 ggcaaagttt atggagcaag ctttattcta acaagcttgc tatgattagc atgatattca
    12301 ttacattatt ctgtcttctt gcaatattag gatataccat agtcaatgat ccaacacctt
    12361 tcagcaacag gcaatgcctt gaacttacag caaaaaagcc tggctaccag gcttcatttc
    12421 ttctgaaacc caataacttt gaaactccca aagtatcatg gtttagcaaa atgatatggg
    12481 gacaacccga cacagcccaa tacatccttg ctgacagcct tgagttttca ggcactacgg
    12541 taattatttt ccgtaaggga aatggtaaat tctttgaaaa agaacattat agagccaaag
    12601 acttaggatt aacaaatatt actgatgtta atacttttaa aaagcatgtt ttacagcact
    12661 tgtatatcac caaacattac cttttaggca ctgacaggct tggccgcgat gtattaagcc
    12721 gtttgatcat aggggccagg gtatcccttt ctgtcgggtt agtttctgtt atcatctcat
    12781 tgttaatagg agtatggctt ggcgctatgg caggttattt tggaggccga gcagacaggg
    12841 ttatcatgtg gtttgtcaac gtatcatggg cagtaccaac acttttactg gttatagcct
    12901 tgaccatggt tataggtaaa ggcttctggc aaatatttat cgccattggc ctcaccatgt
    12961 ggattgatgt aacacgcctt gttcgtggcc aaataatggg ccttcgcgag aaagagtaca
    13021 ttgaagcttg tcatgctatg ggctttggcc acttccgaat tattacaaag catttattgc
    13081 ctggcatcat aggtcctgtt attgttcttt cggcagctaa ctttgcttct gctatcctta
    13141 tcgaatcggg cttgagcttt ctgggagttg gtattcaacc tccagtaccc tcctggggca
    13201 atatgattaa ggaacattac gggtatatca tccttgacaa agcatacctt gccatattgc
    13261 cgggcttggc aatcatgctg ttaacgttgg cttttactat tataggaaat gcgttgagag
    13321 atgcaaccga caccaaagca cccattgctc ccagctaata gcatcattca atagctgata
    13381 aaatctctat tacctctcga tggcttccgt cagcgtttct ttttaagaca aaaaaatcag
    13441 attgcatgtt tacatagcgc ccatctatat gcttcaaacg taaattgcag ctgtaactat
    13501 tattgcaagc agatgacaca tacttttcac gttcacgcac ctttctgtaa tcctcagggt
    13561 gataatgata aatggcttct ttgtttataa gttcctgcaa ctctttatgc ctgtacccca
    13621 acatgcaatg aaaattgcgg ttataccaca acaaactgcg ctttttcacg tctcttacag
    13681 aaactattaa cggcaaacgg ttcaaaacct cttctaacaa gcgcaggtta tcctgcaaat
    13741 actcattttc gcgcgaaaga gctgttaaaa ccatccgaaa cccttcatta tcatgcttca
    13801 attgtttgtt ttcttctgat atgcgaacgt atgattcctg aagtttctga atattttcca
    13861 tagtactccc aaaacgagat attgaaatat cttcatttat aaagcgttta gcttcatcaa
    13921 caaatcgttt ggcagctcct ttcgacatat ttttttttca cactattatc aaatatataa
    13981 aaaatactgt tgaactttca aaaacaaatt attattttga ccaaaaaaaa taattttatc
    14041 ttatttatat tctgcatgtt atatctgaca gccttgcaat tggtgccaac aataaaaact
    14101 tttaatcttg attatttagc attaaaaaca aaatattacc aaaacctgac aaatatttga
    14161 agtaacgaaa agataacctt gttttaacat ttaattaata agcattaata ttacgttttg
    14221 tcaataaatc tggcacattt aatatacttt cgttaaaatt tttagggtac ttgtcaaaaa
    14281 caattgtatt tatataaaac ttttatttat gatcaaaatc aaaaagatct taggtttagt
    14341 aatactgtca atgttggctt tttcctgcaa taaagaagaa ggtgaaggag gaaagggcaa
    14401 cataaaaggt attgtttatc aggtaattgt taacgctcaa acccttgaca ctattactat
    14461 tgcccctgct ccagacgaag atgtttacct catttatggt gatgataaag aaattgacga
    14521 taaaactgtt tcaagttatg acggttcttt tgaatataaa tatttgcgtg atggcaatta
    14581 tcagctattg gtatattcgc aagacacttc aaactattcg aaaaccaaaa cccctgtcat
    14641 aaaaaaagtc catatttcta atggaggtac agcaaaacct gacactttgt ttattctgaa
    14701 aacagtagat tatgataaag gtgccggtat tattgaaggt atagtaaatg ctgaggttat
    14761 tgacaaccaa aacttaaaca ttattgccat tcaggcagcc cctgatcaaa atgtatatat
    14821 tttaaacagc tctacaaacc aagtacttga caaaaccact accagtgacg atggtaaatt
    14881 tgcgttccaa aaccttgttg atggtaatta ttgtattttg gtttactcgc aagatactgc
    14941 caatgcaaaa aacaaaaaag tagccatcaa acagcctgtt caggtcaaaa accaaaacac
    15001 ggtcaaatta tcatccattc atattacaaa aatgttggat atagacgagg gttctggcag
    15061 tattaccggc aaaatttggc taataaacta taagagcaat ggatatacta ttaaagacat
    15121 agcattagcc caagaatatg atgtatatct catatatggc aaccacttag gatttgacat
    15181 gcgcacaagg acaagctatg acggaacttt ttgcttttca aaccttatta atggcaaata
    15241 caccgtattt gtttattctg agcaggaaga catagacggg taccttaccg gggcaactca
    15301 aaaagccatt cataaaaaga cttttgagat taccaacaac aatcaatcat tttatatgac
    15361 tgacaccgtg aaaacttttt aaaacgctat ataacagttt tttatgaaaa tacaactaat
    15421 aaccggacta cttttgcttt ctacggtatt atctgtttct gcacaaaaaa agaaaaaaga
    15481 tatcgttagc aataaaatta aaagcattac agaatttaac gaagattttg aaaaaaataa
    15541 cggtcgctct gtaaaagaaa gctttactgc ttatgacgaa tttggaaaca ttatagaact
    15601 cattgagtat gacgataatg gtaaagaaaa aaaacatatt aaatatgaat atgacgataa
    15661 agacaactgt accaaagaaa cttacctcaa cccaaaagga cagctcgaaa aaactattgt
    15721 ctttaaatat gacgatggat taaaaattga aaaaactact tacctgccta atggcaaaat
    15781 taaagtccgc aaaaagtaca tttaccaatt tcaataaaaa aaatgggaac tatttatcat
    15841 gaaattgaca ctgtattaaa cacttacaag cctatttcac tcagtgaaat ggactctgtc
    15901 aagctgatga gccgcaccga tacaaagttt attttcaatt cagccatgat ttgccaaata
    15961 ttagaaatgg ctaaatctta ttataaagta ttggaaatta acagcacaag gcaatttcaa
    16021 tatattacca catattttga caccccggac ttgtttatgt acaatcagca ccacaatggc
    16081 aaactcaacc gttataaggt taggcaacgc cgctatgatg caaccggaaa tgaatttttt
    16141 gagataaaat ttaagaataa caaaggccgt accatcaaaa gccgtattga aaacaacgct
    16201 aacaatgaac ttaacgctgc tactgataaa ttcttgaaga aaaaaacgcc ttttgaatgt
    16261 tgtcagttga aaaaaacaat tttaaaccag tttatacgca tcactcttgt taataacaac
    16321 atgacagaaa gagctacatt ggattacaac ctttgctttt ctgattttca taaagaacgc
    16381 ccaattccac aattgggtat cgccgaaata aagcaggaca gtaattcagg cccttcgcct
    16441 ttaataacca tcatgcgcga actaaaaata caacccgaag gactctctaa atactgcctt
    16501 ggagttgctt cattatatga cgatgtaaaa attaatacat ttaaaccacg gttaattaaa
    16561 ataaataatt tataaaatgg atacacctac tattgaaaca atgaaatttc tgggcattga
    16621 actttttgac aaaaaagatt tttttgagct tttaattcgt ttgtccttca atttattgat
    16681 tgttgggatt atagttcgtt tactttatta cccaattaca cgtcgcaaag actacctgtt
    16741 tacatacatt ctcctgtcaa caatagtttt tcttatttgt tttatgcttt ctaatgtaaa
    16801 actacaaatg ggctttgctt tagggttatt tgctgtattt ggtattttgc gttaccgcac
    16861 caacccaatg ccaatcaaag aaatgacata cctttttgtt gtaattggca tttcaattat
    16921 caattcacta accaacaaaa aggtaagcta cgctgaaata ttgtttacga atatagctat
    16981 tgtgtttgta atctatggat tagaaaaagt ttggctactt agacacgaat cttgtaaact
    17041 aattaactat gaaaaaattg agttaatcaa gcctgaaaaa cataacgaac taatggcaga
    17101 cttacagcaa cgcaccgggc taaaaataaa ccgcatagac ataggtaaaa tcgacttttt
    17161 gcgcgacact gcccgtatta tgatttacta ttatagtgac gagaaccgta tcaacttagc
    17221 tgatgaaggc cgcgtatata ctggagatgg cgatgatgat taataaaata aaaaatatct
    17281 tgctttgttg cttttggggg gcactttctt tgactttggc agctcagcaa gattttaacc
    17341 aatggagtac cattgacttc caatataatt tcaacaaacg aaatgccgtt tgggtttcaa
    17401 atgaaatgag gctcaacgaa aatgcctcac aatttagtaa gtacaacttt gacgcaggat
    17461 atgaccgcaa aatatgggaa aaattatatg caggcgtttc atttcgctat tcaagggtaa
    17521 acgacaaatt tgtttacaaa aacggctatg attacaacct ttatgcagaa tacgaatttt
    17581 cgtaccaacg ttattcttta gacgttaaaa taaggtttca gcacacctct cagggcatcg
    17641 accttgaaac tattgaagaa gtagagaatg tttggcgtaa taaagctgac ataagctaca
    17701 aaataccata cacccacctt tcaccttata ttgcttttga atatttctcg gaaattacag
    17761 acaaaggatt gtggccccag aaataccgtt tatttggagg tgtgaaatat aacattaaca
    17821 aatccagtgc tatatcagct tattacggat tgcaatattc tttgcgtaaa cccaatgaaa
    17881 actatattct cggcttgaaa tattctttaa aactcaaagc caaaaaaaac aaagacaagg
    17941 aagaagatac ctctgaaaat gaagaatagc cctatctttg ggcaagcacc ttatctatat
    18001 ttgattttag cttttcaaaa gaagcccgtt ttactggggt cccttcaaac attttggcat
    18061 actcaggttc tgtaagcgat tgccattgtt ctgctgtcaa gtctccccac ttttttgcac
    18121 tttctgcaac cctttcatca aaagcaacgg ctttgctatt ccatgggcaa acctgctgac
    18181 acaggtcgca accataaata aaaccactaa aactattatg aataggcttt tcactattta
    18241 ttttcatctc tatagtttga tatgaaatac atttatttga gtcaatcaat gaattaccta
    18301 taatagcatt ggttggacaa gcttctatac atctttgaca agttccacaa cgattttcaa
    18361 caggattatc gtatttatcc gcctgctcat tgatgatgat agtgccaata aaacaatttg
    18421 tacccaaaac tgggtttatg agcataccgc tcttaccaat ccatcccaag ccggcctgtt
    18481 cagcaaaata tctttcggct acaggtgcag aatctacaaa atatcgacct tgtaattccg
    18541 gatatttttg ctgcaaagca ttataaaaga attttaattt ctttttcagt gttttatgat
    18601 aatctcttcg ccatgcatat tgagcaatac gaaccgggct tttttcaaac acatcaagct
    18661 ttgtatgata gctatataaa actactacaa ctgtagaagc attttcaaca agtaactgag
    18721 gtgtaaaacg caaatgaaca ttggtttcca tccaatccat gctggcatga aaacccgctg
    18781 acaaccattt tttataaact aattgggtat tggcatccac ttttgccttg gcaagcccac
    18841 aagcatcaaa gccatttttt tgggctaatt gtattatttc atcatgaatt aacatgctta
    18901 aagaagtcca agctcaagca ttgcttcttc ggtcatcatt tcttttgtcc agggtggttc
    18961 aaaggtcagg tttaccttca attcattaac cgcaggaata gcttttattt tttcatgcac
    19021 ctcctgcacc agctcgtctg ccatagggca atttggtgca gtcatggtca ttactatctc
    19081 tacattgcgc gactcatcta catttatttc gtatattaaa cccaaatcaa agatgtttac
    19141 aggtatttct gggtcatata tcccacgcaa cacagtagca atctctgttt ctaacattaa
    19201 tatcgggttt tccatatcct taatttttaa aataaacagc tttacagctt atttgttttg
    19261 ttccattttt gttttataag ccaaggcaaa catgcgcatt tgctttatca tagacaccaa
    19321 accatttgcc ctggttggcg aaaggttttc tttcaaccca attttatcaa taaactcaag
    19381 ctctgcacta ataatttcgt caggctcacg attagaaagc accctaatca acaacgctat
    19441 aatgccttta gtaatgatag catcgctatc tgcagtaaaa acaacctttc cgtcaatcaa
    19501 ctctccacta agccataccc tgctttgaca accttttatc aggtattcgt ttgccttgta
    19561 tttgttatca agcggctgta attctttgct tatctcaatc agataattat atctgtccag
    19621 ccaatcgtca aattgaagaa actcttcaac aatggcatct tgtgtttctt gcagcgtcat
    19681 cttttttttc ggcaaaatta ataaaacttc agcatcagcc aaacatggtt tttaccctca
    19741 acaatccttc gtatagcctg tcaatttctt caaaagtatt gtaaaaagcc attgaagccc
    19801 tcacagttcc ttctatttca taaaagtcca taataggctg ggcacaatga gtgcctgtcc
    19861 gtactgccac accaagcttg tccagtatca tcccggtatc gtatggatga atattttcaa
    19921 gcaaaaaaga aattactggc cctttatccg ctgccgttcc ataaattttc agcccttcta
    19981 tcgtctgaag cttattggta gcatattcca aaaggtcttt ttcatatcct gcaatattat
    20041 caaaccccag cttctttata taatctatag ctaccgataa tcctacagca tcaacatagt
    20101 ttgttgttcc agcttcaaat ttatagggta aagaattata ataagtcttt tcaaaggtta
    20161 cacaatcgac catatctcct cctccctgat atgggggcat ctggttgagc cattcttctt
    20221 tgccatacaa aatacctatt cccgtaggtg catatatttt atgcccagaa aatacaaaga
    20281 attcacaatc cagctcctga acatcaatct taacatgctg aatactctgg gctccgtcaa
    20341 taagcaccgg tatccctttc ttgtgggctt tatctataat agcctttata ggattaaccg
    20401 ttcccagaac atttgaaaca tgcgtaatcg caagtatttt agtcttctcc gttaaaagtg
    20461 aatctactgc atccagtttc aactccccat tctcgtccac aggaataacc ttcagctttg
    20521 cattcttgcg ctcgcaaagc atttgccacg gaacaatatt ggcatggtgc tccatagccg
    20581 aaatgaggat ttcatcgcca gctttgacaa acttttcgcc aaaagaaaaa gctatggcat
    20641 tgatgcttcc tgttgttccg ctagtaaata tgatttcctg ggaatacctg gcattgataa
    20701 attgctggat atttttgcgg gcattctcat acatttcggt agattggtcg ctcaaataat
    20761 ggactcccct gtgaatattg ctatttgttt tggtataaca ctcaacaaca gcatctataa
    20821 cacattgagg cttttgagtt gttgcaccat tgtcaaaata agccaaaggt ttgccatgaa
    20881 cagtgctacc cagtattgga aagtcttttc ggacagaatt aacatcaaac atagaaaaaa
    20941 tattaagact caaagttact acaaagcctg acaattgtgg caacgggtca actccttgcg
    21001 caatctctta ttcacaattt catcaatcct aaacctcagg ttttcgaact ttatattttg
    21061 tacaatctcg tgtgcaaaag caaacataag caacatacga gcttcatctt tcgaaatgcc
    21121 tcttgctctg agataaaaca atgcgtccgg gtcgatttga ccaactgtag ctccatggct
    21181 acattttaca tcatcggcat atatctctaa ctgtggacgt gtattaactt tagctgtatc
    21241 tttaagcaac aggttgttac atttctgaaa agctgctgtt ttttgggcat cccgggcaac
    21301 cattattttg ccattaaacg atcctactga tgtatcatca agaatcccct taataagctg
    21361 attgcttgta cagcttggct tggcatggtc tataagtacg ttgaaatcta ttatttggct
    21421 accatcagtc atgtataagc cattagtgct attatctgca cccactccat caagctttgc
    21481 cgcaatatta ttgcgtatca tacccccatg caaagacaac actgtataat taactctcga
    21541 gttagccttc tgcatgatat tggtatgatt gatagcccct gcattgttat ggatattctg
    21601 catattagta acatcaagct gagccccttc tttcaccaca atttctgtaa gctggttaga
    21661 aagaaattta ttttgggaca acgtatgatc acaaataaca aggtttactg agctatttcc
    21721 ctctaaaaca atgagattac gaggagatac aaacatatca tcctgtgcaa gttgcaagtt
    21781 tattatctgt atagaagaat gaaagttaca atttttagga agataaataa aaatcccatc
    21841 acgaaataca gccttattca gcgaaactag gctatcagca agatttacat cgacatctaa
    21901 ataaggaatt accttttctg gtatttcctg taaagctttt gccaaactgc caatccaaac
    21961 acccaaaggc aactcatgag gctttgtatg cgaatgaaaa aaaccattga tggtaatgat
    22021 ttcatgcata tcaaaccttg gaacatcgca ttgaaaaacc tgttcactat ctagcttaag
    22081 cttttcaggt tgaatggtat atccgtaagt atggtcaaac cagggagtca aatcaagata
    22141 tttatatctt tcatcagatt tattaggaac tcccattgac tcgaaaaccg aaactgcctc
    22201 ctgacgatga tttgacaatg catcagaaaa cactcctttg ttttgatggt aaattcccga
    22261 gtatgttttt atcagccttt cgactgttgt aatagtttcc attatctact ttatgaaaat
    22321 attaaccaat ccgctaaaca gttttcttaa tccagtcata gcctttttct tcaagctcca
    22381 gcgcaagttc ttttcctgca gtctttacta ttcggccatc ataaaggaca tgaataatat
    22441 caggcactat ataatcaagc aatcgttgat agtgcgttat aacaatggta gcattatcag
    22501 cagttttaag cttattcact ccattcgaca caatccttaa ggcatctata tccaatcctg
    22561 agtctgtttc atccagaatt gacaaggctg gatttaacat agccatctgg aatatctcat
    22621 ttttcttctt ttcaccacct gaaaaacctt cattaaccga acggcccgta agtttgccat
    22681 ccatctccac aagctgttgc ttttcacgca tgaacttcag aaaatctgca gacgaaagtg
    22741 caggcaaatt attgtattta cgaacttcat ttactgaagc ccgcaaaaag tttaccatac
    22801 tgacacccgg aatctctacc ggatactgaa aactaagaaa caaaccttct ctggccctgt
    22861 cttccggcga aagttcaagc aggttcttac ctttaaacca aacttcacca tgggtcactt
    22921 ctacattctc acgcccggta agcacagcag ccaatgtact tttgcccgag ccatttggcc
    22981 ccatgatggc atgtacttct ccagctttta cttcaaggtt tatccctttt aatatttctt
    23041 tttcgccaat cttggccctt aagtctttta tgtgtagcat atcacaaatt tttctaattc
    23101 tatatattgt ggtaaagtag aatagtagtt aagtagaata gtagctagac atctacttca
    23161 acgtgaaaca caaactatta agcatgcgat ctacttcttc ctgtaactct acaattttta
    23221 ttttttgttc aacattttat gtaacctaaa tcaaatacca gtaatgaata atatgaacac
    23281 tcatccaaag aaccctgaga tatattatag taccgaagct tctcagtatt agttttcttt
    23341 ctaaatcctt cagcaatatt tgcagcaaca gaaacacttg cccttctata ttgagaagtt
    23401 aatccataaa cttcttcctt tggaaaagca gaagaaactt tatatactaa taaaacatat
    23461 tcgtgggcct tttgccacac tatcagatcc ctatatgttt tagcaggtaa catctatact
    23521 ttactacttc tatactttac tacttcttcc taccccacac taccttccaa gcttatctgt
    23581 agcaatttct gtgcctcaac tgcaaattcc atcggcaact tattaagcac ctctttggca
    23641 tagccattta caataagccc tacagcatct tcagttgaaa tgccgcgctg gttacaataa
    23701 aatatctggt cttcaccaat cttagaagtt gtagcttcat gctctacaat ggcattaggg
    23761 ttttccactt ccaaataagg gaatgtatgc gccccgcatt tatcgccaag caataacgaa
    23821 tcgcattgcg aaaaattacg tgcattatca gcacctttaa gtactttaac cagtcctcga
    23881 tagctattat tgctctttcc ggccgaaata ccttttgaaa ctatcctgct acgcgagttt
    23941 ttaccaatat gaatcatctt tgtcccggta tctgcctgct gataattgtt tgtaacagcc
    24001 acagaataga attcacctac agtattatcg cctcgtaata tgcagcttgg atatttccag
    24061 gtaattgccg aaccggtttc tacctgagtc catgaaatct tagagttatt ccctttacaa
    24121 ataccacgtt ttgttacgaa attataaatc ccgcctttcc cgtttttatc cccaggatac
    24181 cagttctgaa ctgtagaata ctttatttca gcattctcca tcgctaccaa ctctacaaca
    24241 gcagcatgca actggttttc atcacgcata ggcgctgtac aaccttccag ataactaaca
    24301 taagcgtcat catctgcaat taacagtgta cgctcaaact gacctgtatt tgccgcattg
    24361 atacggaaat atgtggacag ttccatcggg caacgcacac ctttagggat atagcaaaaa
    24421 gagccgtcgc taaataccgc tgcattaagg gctgcaaaat aattatccga aaaaggcact
    24481 accgaaccca aatatttctt taccaaatca ggatgctctt tgattgcttc actcatcgaa
    24541 caaaatataa tacctttttc agctaaagct tcacgaaaag tggttttgac agaaatactg
    24601 tccatcacag catccaccgc aactccagcc aaatgggctt gttcgctcag cggaattcca
    24661 agtttattga acgtttccaa caactcaggg tcaacttcgt ccatgcttga atactttgct
    24721 tgttgacgag gagctgcata gtagataata tcttgataat ttatttcagg tatctgcaaa
    24781 tgcgcccaat caggcatttt catcttttgc cagtgccgaa atgcctttaa cctgaactca
    24841 agcataaact ccggttcttc tttcttttta gaaattaacc tgattatatc ttcattaagc
    24901 cctttgggga tagtatctat atcaatattt gtataaaaac cgtatttgta atcacccccg
    24961 gtcacatcct ggatgatatc ttgtgaattg tcagacatac tattcttatt tagattattt
    25021 ctaaacagtg caaaggtaat aaaagttcaa aacttaatta gtgattttga taatatttaa
    25081 gcatttaaat actttaattt tagaaaatta caattaaata ttgattatca gtgctttatg
    25141 aaatatttct tgataaaatc taacttggca cgcttcttga aattatataa ataaaacctg
    25201 cgcaggtatt taaaaatttc ttaattaacg gggcttaagc tacaatatgt aactgaaaag
    25261 caccaattgt aggacttttt aataccttaa actatgcaaa caaatgaaaa cgctgcattc
    25321 acgaaaacag caattaactt ggatgaaata gttttcgcca accgtaacca cgcatacggt
    25381 gcatacaata tgcgaaaaga atataagaaa agacttctca tcggttttat tgccacattc
    25441 cttattttta gcgcttctat tggcatttcg atgtataaaa catataccaa tataatagtt
    25501 ccaaaacctg agataaaaca cgatgtcact ttcgcacccg acaaattaga caaccctctg
    25561 cctccccctc cagccccggc tccaataatg ccaacaattg aaagatttac ggctcccatt
    25621 attgttgata ctacagacga aaatgacctt acccttataa gtatggatga agccacagca
    25681 ttgacaacca acgataccga agacttaact tatgtaccag aaaatacagt taatacctat
    25741 actccagaaa ttgacgagcc agaaattggc cgttgggatg tcacagaaca agccacattt
    25801 gaaggtggcg atttaaaaac ctttcatgcc tggctgcaaa tgaacgtgac ataccctcaa
    25861 gaagcaatag aagcaagact ttctggtaga attacattgt ttttcagcgt caatacaaaa
    25921 ggtcaggtct gcgatgtaaa agtcatcaaa ggcatacacc ccgtacttga tcaagaagcg
    25981 ctccggacaa tccttaactc tcctgcatgg aaacctgcca aatttaacgg aaagcctgtt
    26041 aaacaaaact tcacagtacc attaatattc cgtatcgaat aaataacagc acattcaact
    26101 attccaaaac cctgcccaaa agcagggttt tattctgtat aaacattact aaatataacg
    26161 cctaacaata aagatttttc agttgttaat attgtaatat ctttattata aaaccattac
    26221 caataaatca aatactaaaa accgcaattc tattatatat tttataaaaa tttttatata
    26281 taattttgaa attcaagata aagcgtatat gtttgtagtt aaataattga agtaaaaaca
    26341 ttttcaacat cagacaacca ataacttaac actattcaga catttcactt attatgccat
    26401 acaaagaaaa aaaaatagaa aaagtatatt acaccatagg agaagtggca gaaatgtttg
    26461 gcgtaaatac ttctctgatc cgtttttggg aaaaagaatt tgatgtcatc aaacctaaaa
    26521 aaaacaaaaa aggcaaccgc ttatttacca aaaaagacat tgacaatttt cacaccattt
    26581 accatcttgt taaagaaagg ggaatgacac taaaaggcgc ccaaaaaaag atgaaagaaa
    26641 ataaagaaga taccgaaaac aattttgaaa taataaaaac cctgaaagcc attcgcgaaa
    26701 tgttagtcga aatacgcgaa aatatgtaaa tatgcaaatc aaagaaattt taacaaccct
    26761 ggaagatttt gccccacttg cttttcaaga accttatgac aactcaggcc tttgcattgg
    26821 caaccccaac aaagaagtca aagcccttct gcttaccatc gatattaccg aagatgtcat
    26881 tgatgaagcc ataaaacttg gatgcaacct gataatttct catcatcccc ttattttcag
    26941 cggtataaaa agccttaccg gacaaaactg cattgaacgc tgtattatct atgccattaa
    27001 aaatgacata gctatatatt cagcccatac caacttagat atagccccaa tgggggttag
    27061 ttacatgatg gcccaaaaac ttggcctgaa aaacatcaaa atcttgtcgc cgttagaaaa
    27121 taagcttctc aaattggtct cttttgttcc ttttgaacat gccgaaaaag tcagagaagc
    27181 aatatttgaa gccggcgcag ggcacatagg caactatgac aggtgcagtt acaaccttga
    27241 aggcaacgga accttcagag gtaatgaaaa ctcaaatcct tttgttggca ttagtggcca
    27301 agaccataca gaacccgaaa taagaataga gactatactt cctgcccata taaaaaataa
    27361 agttttaaaa gcattactta atgctcaccc ttacgaagaa ccagcctacg atatatatcc
    27421 gttattaaac acatttgaaa aagcaggttt gggagctatt ggcaaaatag agcctgtcga
    27481 agaaaaagta tttctgaaaa aaattaaaga agtatttaat gtaaccatag ttagacatac
    27541 acctttgcaa aataaaaaaa ttgaaaccgt tgccctttgt ggcggaagtg gcagcaattt
    27601 gctcaaaaat gccataaaag aacaagctga cattttcata agcagtgact tcaaatatca
    27661 tcaatttttc gatcacgaaa agaaaatact cattgctgat attgggcact ttgagagtga
    27721 acaattcaca aaagaaattt tttatcaaat aataaagaaa aaatttgcta attttgcagt
    27781 tcatttttcg agcattaata caaatccaat aaattactat taagtttatg gcacatacag
    27841 aaagcgaaat ttcaatagaa gaaaaactaa gggctcttta cgaactgcaa cgtgttgaca
    27901 gcgaaattga caaaatcaaa attttgcgtg gtgagcttcc tcttgaagtg caagatttgg
    27961 aagacgaaat agcaggatta gacacccgtt tacaaaacct cgaagaagaa gtaaaaaatc
    28021 tcgatgtagc catttcaacc aaaaagaacg aaatagtaaa cgccaatgcc cttattaaaa
    28081 gatacaaaga acaacaagaa aatgtaagga acaatcgcga attcgattct ctttcaaaag
    28141 aaattgaatt tcaaaccctc gaaattgaac tatgcgaaaa acgcatccgc gaatttacag
    28201 cccaaataaa gaacaaaaaa gaaatcatcg aaagctcatc agctactctt aaagaacgca
    28261 ataacgatct taaaaacaag aaaaaagaac ttgaagaaat tattgctgac acccaaaaag
    28321 aagaagaagc tcttttaaaa aaatcaaaag aaattgaaaa acttatcgaa gcccggctga
    28381 tgaatgcata caaaagaata aggagcaatg cgcgcaacgg gcttgccgtg gtatctgtag
    28441 aacgcgatgc ttgtggtggt tgtttcaaca aaattccacc tcaaagacaa atggaaatca
    28501 aagctcacaa aaaaatcatt gtatgtgaat attgtgggcg catccttgtt gataaaaatt
    28561 attttgaaag tcaaaactaa caactattga tttaaacaat aaaaaaaccg ggaagaaatt
    28621 attttcccgg tttttttatg ccccagaaac aaaaaaacat atcccacttt ttctcaatgt
    28681 tcatattttc agttttttag caactaaaat tgacagaaat aacaagaaat tccaacaaca
    28741 ttttttttgt tattttataa acaaaaagtt ctgtgaaaat tataacaata caaatacatt
    28801 ttttactttt gcataagttt aatcattagg ttagtgaact cattcaaaac aactgaatga
    28861 gttgaaaata aagataataa taataaatat gaaagtaaca gtagttggtg caggaaacgt
    28921 tggggctaca gtagcccagt gcattgcaga aaaagaatta gtaaatgaaa ttattttact
    28981 tgacatcaaa gaagggttat cagaaggcaa agctcttgat ttgtggcaaa cagccccggt
    29041 taattactac gactcaagaa ttaaaggtgt aacaaacgac tacaaaagta cagaaaattc
    29101 tgaagtcatt gttatcacct caggtgtacc tcgtaaacca gggatgagcc gcgacgattt
    29161 aatttcaatc aatgctaata ttgtaaaatc tgttgcagaa aatgccatca aatactctcc
    29221 caatgctaaa attattgtag tgtcaaaccc tttagatgta atgacctatg tagcatacct
    29281 tgctgctaaa acttcgccag aaaaagtttt aggcatggcc gggattcttg ataccgcaag
    29341 atatcgtgca ttcttagcag atgaactaaa atgcaaccct aaagatatcc aggctttatt
    29401 acttggcgga catggcgaca ctatggtccc tctcccaaga tatacaacag tttcaggtat
    29461 ccctgtaact caacttattg aaaaggaaaa acttgatgct ataatcaaac gtacacaaaa
    29521 cggaggtggc gaaattgtaa gtttactcgg cacttctgct tggtatgccc caggggcatc
    29581 agctgctcaa atggtagaag caatcattcg caaccaaaac aggattttcc cttgctgtgt
    29641 taaacttgat ggtcaatatg gctacaaaaa tgtatttgta ggcgtacctg ttaaagttgg
    29701 caaaaacggt gttgaacaaa ttattgaaat tgagcttaac gccgaagaaa aagcttcttt
    29761 tgacaactct gtttctgcag taaaaacatt gatggatgtt cttgacaata tgaaactttt
    29821 ctaaaaaagg cttacttgaa ataaaaaagg ctgcacagtt gtgtagcctt ttttttgcgg
    29881 aaagagaggg attcgaaccc tcggtacagt tgcccgcaca acggttttcg agaccgtccc
    29941 gatcgaccac tccggcatct ttccttaaac ggctgcaaat ttaggattaa aatttaatat
    30001 ttgacacttt acattgggca aaaaaaaaga gctgcccttt ttagaacagc tctttgattg
    30061 atttgctatc ggataaataa taattctcta tattttggca aaggccattt ttcgttgtca
    30121 actataagtt caagtttatc aatatgatac ctgatttctt caaagaatgg gacaactttt
    30181 tcataataag ccatggccat ctcattaaca tcttcaatct tattagccac tttacgcgct
    30241 tcaatcattt cactaacctt gctcttaatt gtcgaaatat gtccattcac ttccttaacc
    30301 agctctatac gctttgcaga aagctcttta aattctttct cgctgaagat gtctttcata
    30361 gctttaatat tctcaattaa agatgtttga taatctacag caacaggaac tatatgattc
    30421 atagccaaat cacccaacac acgagattct atctggattt tcttcatata aatttcctga
    30481 cggatttcag cccttgcttc aagctctttg tgcgaataaa cttttgtctc accaaataat
    30541 cttgcagctt ttggagatgc ccaaacttta ttcaaaacag gtacagcacc ttcggtattc
    30601 aaccctcttc tcttggcttc ttttttccat tcttcactat aaccattacc atcaaaacga
    30661 attgcttttg actcactaat gtaacgggcc aaaatctgaa ggattgcatc atctttcttg
    30721 attccttttt taataagagc atcaacatct ttcttaaata tagtaagctg ttgagcaaca
    30781 gcagcattca aagctgtcat ggctggagca cagtttgctg aagaacctac agctcggaat
    30841 tcaaaacggt ttccggtaaa tgcaaatggc gaagtacggt tacggtctgt attgtcaagc
    30901 aaaatttctg gaatacgacc tatgtcaagt ttgagagcag ttttatcatc cgcacttaat
    30961 tttgaacctg taactttttg ttcaatttca tcaagcattt tagagatttg ttctcccaag
    31021 aaagccgaaa caatggcagg aggagcttca tttgcaccca gacggtgggc atttcctgcc
    31081 gaaacaatag aagctttaag aatatcgtca ttgttgtgaa ctgctttcaa tacattaaca
    31141 cagaaggtca aaaacaataa gttcccttta gggtttttcg atggcgaaag caaattaacc
    31201 cctgtatctg tccctaatga ccagttgttg tgcttccctg aaccgttaac tcctttgaat
    31261 ggtttttcat gcaaaagtac ttgaaaatga tgctttttag caaccctgcg cataatgctc
    31321 attattaaca agttgtggtc aactgcaagg ttgcactctt cataaacagg agctatttca
    31381 aattggtttg gagcaacttc attgtggcgg gtttttgccg gaataccaag tttccaggct
    31441 tcatattcaa gctcttgcat aaatgctaaa acccgctcag gaattgaacc aaaatattgg
    31501 tcttctaact gttggttctt ggcagaatca tgtcccatca atgtacgctc agtaagcatc
    31561 aaatcaggac gtgctaaata taaagattgg tcaatcaaaa aatattcttg ttcccaacct
    31621 aaaaatgaat atactttctg aacatcttta tcaaagtatt ggcaaacagc aacagctgcc
    31681 tgatctactg catgtaaagc cttaagaagc ggtgctttat tgtcaagcat ctcaccggta
    31741 taagacaaaa agatagtagg aatacaaagt gtctggtcta aaataaatgc tggcgatgat
    31801 agatcccatg cagtataacc acgggcttca aaagtgctac gaatacctcc gcttgggaaa
    31861 ctcgaagcat caggctcctg ctgaacaagc aatttcccgg tgaaactttc tattacgctt
    31921 ccattcccgg ctggctctgc aaatgcatca tgtttttcag ctgtcccttc agtaagtgga
    31981 tgaaaccagt gtgtataatg agtagctccc atttccaaag cccaagcctt catacccgcc
    32041 gcaacctgat ctgccatttt gcgatcaata caaatgcctt tgtctatagc aagttctact
    32101 gcctgatatg cctcttttga aaggtacttt ttcattttag gtttatcaaa cacatacatg
    32161 ccaaaatatt ctgaagcttt tgttgcagga gctttaacct ctacaggttt acgattaaat
    32221 acctgttcta atgctttaaa ccgaagatgt gccataacaa ttgatttaaa atttgtttaa
    32281 aattggattt tatggtacaa aaatactatt tttttataat tatcaccatt tttttataat
    32341 ttatttgatt tttattttat ttttttaacc tatttaatag tattagtttc attttttaac
    32401 atattggcag tttttaataa agtattaata aatttttatt aaatataatt attcgcaata
    32461 tcaagtcgta ctcaggctca acacttgcaa acgattactt attactcaat tagtccattc
    32521 aaaaatttcc aaaatctcaa caaatctaat atgcttacaa gctgaatttg aatagaaaag
    32581 aacaaaataa tattaatcat taccatatat atacatcatt ttaaaaacat aaatattttg
    32641 aaaagacaaa taaaaattat aaagtttatt atcaaattgg ccaaacaatt attacttcaa
    32701 cccatttttt tgtaaaaaaa taaaccaaaa accaataata attaaattgc taatggcatc
    32761 cgcataacat tttttatctt tgttgcttat aaatatacaa taaacgtgaa aatacttata
    32821 attggagccg gaaatatggg ctcttggtta gttgaagcgc tctgtttaga acacgaagta
    32881 gctgtttttg ataccgataa aaaaaagttg aaatttctgt ttaactcaca cagattgacc
    32941 caacttagtg agattagcaa tttccaacct gaattagtta tcaattcagt gagcttgcaa
    33001 tatacgtttg cagcatttga aagcatcttg ccatatcttc cccaaacatg cataatttcg
    33061 gatatagcaa gtgtaaaaaa cggattattc gacttttata aaaagtcagg gtttaggttt
    33121 gcctccattc atccaatgtt tggccctaca tttgccaata tcaaagaatt ggctcaacaa
    33181 aatgccatta tcataaaaga atcagaccca gaggctaaag aatttttcag aaacttcttc
    33241 caatctttac atttgaacat ttttgaatac acctttgaag aacacgacca aaccattgcc
    33301 tactcgcttt ctgtaccttt tgccacaacc ttagcatttg catcgtgtat caaaaaacag
    33361 gaagccccgg gtaccacttt caaaaaacac atggttatag ccaaaggact attgtccgaa
    33421 aatgactatt tgatttccga agtacttttt agcccttatt cactcaagca gattgaaaac
    33481 attaaaacca aacttgaaat gctcgaagaa atcattaaaa acaaggatac caaagcaatg
    33541 caccattttt taaaaaatgt aagaaaaaat attgaataac aggaataaaa taacgctact
    33601 gatatatatt gtattttcat tactgtccca ttaaaatcta aaagagttct ttgaaaagtt
    33661 tgaaattta
//
LOCUS       DAOSPF010000037        32583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1396_length_32583_cov_7.798942, whole genome shotgun sequence.
ACCESSION   DAOSPF010000037 DAOSPF010000000
VERSION     DAOSPF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_32583_cov_7.798942"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..608)
                     /locus_tag="PK252_08605"
     CDS             complement(159..608)
                     /locus_tag="PK252_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT14807.1"
                     /translation="MQVIIEKVSLNEVARCCELLNQLFSQDLEFVPDNEKQTAGLTQI
                     IDNPNIGEILVLKKNNKVIGMVSLLYSISTALGGKVAVLEDMIIDKEHRTSGYGSMLL
                     EKAIAFAKERGCLRITLLTDYNNDVAIHFYKKYGFEKSQMIVMRQLL"
     gene            821..1144
                     /locus_tag="PK252_08610"
     CDS             821..1144
                     /locus_tag="PK252_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT14808.1"
                     /translation="MKIYFLCFLCLITLFSVAEAKEITIKKLPAGNSIFIDGDDDDWP
                     ATAVWQAVDQHKGEPANKNNTVSLKMTYDDDFLYVAVKVLDGTIDTAQSTNAWEKDCI
                     EVFLY"
     gene            1183..2139
                     /locus_tag="PK252_08615"
     CDS             1183..2139
                     /locus_tag="PK252_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14809.1"
                     /translation="MYGAWQLRKVYGRPMTDLPGKGFQAEETDIAGGYLQEWKLSWDT
                     LASPSYGAYWGGKKFRFEILNQDNDGAGLNSQLFWNSNAYDQWNTIKNQGTVYLDYSP
                     SDDLVLSTSSFNISAFFKIFSVEVYTADAWSAQSNKSWLTLENTSGEGNGTIKFSVSS
                     NNSELSREAKITVSNGKVSKVVIVTQAGVSCSSLLAKTKLELQEARDSINMLLDKVAR
                     YDSITVAVVEMKDFTAIVKLPETNVSLKLYPNPTKDKVLVECESLISKIEIFNIKGQK
                     VYDKISSANTSYLRLEGFKAGTYILKVHTDKGVVGGIFVIKR"
     gene            2339..3598
                     /locus_tag="PK252_08620"
     CDS             2339..3598
                     /locus_tag="PK252_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HOT14810.1"
                     /translation="MLAFVLIISTETIANAQSSAGSGAYATKKYRNLFAENGHSPDSI
                     NLKLEKAFQQLFYGDSAKRLYFVSGKNQNGLLANFRDVYSNDVRSEGMSYAMMIAVQM
                     NKKEEFDALWNWAMTYMYVRKEGHPSKGYFCWSLKPNGTPNFEVAAPDGEEYFAMSLY
                     FAANRWGNGQGIYNYKAYADTILLTMRHHPVVKGRTINEEVKVGPMVNEKYNMILFAP
                     DSGKNHFTDPSYHLPAFYELWAMWGPEPERTFWKAAADTSRAYFRKAQHLKTGMSSDY
                     ANFDGTPHPIAWNPHSHHFSWDSWRTMSNWSVDWSWWRKDTMQQVLSNRIQAFFASQG
                     IDKFGSQFELDGKVINPQHRKGLVATAAVASLAATHPIAKDFVEAFWNIPIPEDHGER
                     YWDGSLYLLSFLHCSGNFRIWEPKKVD"
     gene            4313..6448
                     /locus_tag="PK252_08625"
     CDS             4313..6448
                     /locus_tag="PK252_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT14811.1"
                     /translation="MKNEIFKIKNKTLQLVSISLILFCPFSLKAAEPSPAPGSAAAEG
                     KLKSVSGYIKDASDGEALIGATVLVRELKKGAAANTYGFYSVSLLPGKYTLDFSYVGY
                     KTETKTIELKENTTLNIELSNETKEINEVVVKAESTNDKLKRPEMSVQKLEMKTIKKV
                     PALMGEVDVIKVIQMLPGVVSTSEGSSGFSVRGGGIDQNLIILDEATVYNASHLMGFF
                     SVFNNDAISNVKLYKGDIPANYGGRLSSVLDVRMKEGNMKKWSAAGGLGLIASRLTIE
                     GPIAKDKAAILISGRRTYADLFFPLATDKNLKKSKLYFYDLNLKGNAIINQNNRVFVS
                     GYLGRDIFGQKGAGDIGFGNKTATVRWNHQYSGKLFSNLTGIASTYDYDLFQSLDSTE
                     YSWKSNLTDYGLKLDFNWFANTDNEIKFGVSSTYHTITPCDAYSKSSTVMQYFPKKTS
                     NSYEHGIYATNQQKIGDKLTLKYGIRYSIFQNVGKGIIDRFDDNHNYIKSDTFKAGKL
                     INTYTGWEPRLGVNFTINDVSSVKASYSRTYQYLQLASNSNGGMPLDYWFPASPNVKP
                     QESDQYGLGYFHNLFDNTLETSVEFFYKDMRNVIDFRDHADLLFNDRLEGDVRTGKGY
                     SYGAEVLIRKNEGAFTGWISYTYSRAFRKVAAINEGKEYQAPYDKPHNVNLVLNYEFT
                     PRISASANFVYATGTPMTKPLGLMKLMTM"
     gene            6448..6717
                     /locus_tag="PK252_08630"
     CDS             6448..6717
                     /locus_tag="PK252_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14812.1"
                     /translation="MFIYSDRNSSRMRDYHRLDLSLTIKDKGAPDRFWHGEWVFSVYN
                     VYGRHNDWMINYQNKFEGTSSEVKEATRTYLPFLFFPGITYNFYF"
     gene            6733..7623
                     /locus_tag="PK252_08635"
     CDS             6733..7623
                     /locus_tag="PK252_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOT14813.1"
                     /translation="MKKIKNKLKLALYIIPAIFITACEEPIEDKLDSTYTRLVVDGTI
                     TTDTMAHKVLLKYSNDALNRNPIRFVSGAVVTISDGTNTFNLHENPKVPGEYLTDSNV
                     YGVAGNTYTLNISNVDVDDNGVKETYTASSYLPYMSRIDTITVTDYIHNDKLRGWLIN
                     LYGQDRGGRNYYLTKAIRNDTLVTDSLKEYGAAANSGFEGEKYEGFAVYFLEKDDTTD
                     IVRENDRITLELNCITKDYYDFVIGFQKEYFPKNPIFSGPSANVTTNISPKEKAVGFF
                     AAYSVVRKSTIYKKRNKKYD"
     gene            complement(7745..9505)
                     /locus_tag="PK252_08640"
     CDS             complement(7745..9505)
                     /locus_tag="PK252_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOT14814.1"
                     /translation="MKTQVLKHISFFISIMIVLMAFAACEKDQDSETQSDDQSKTTTF
                     TGVQEDSTDYVRDTASELNIVLNGSSATTDAKGVTINGSVVTITTAGTYKVSGSLTDG
                     QLIVSATKDDVVRIILNGADITCSNSAPIFVQKASKVLIMLSENTNNILTDGASYILA
                     NADEDEPNAALFSKTNMTLFGTGSLTVKANYKDGISSKDGLIIKSGTYTVTAIDDGIR
                     GKDYLVINNGNFTIKSGGDGIKSDNTSDAQCGFVNITNGIFNINAVADGISAEKDIVI
                     TDGTFNITTSGNANSISAKGLKGTNLVEINGGIFNITSTDDAVHSNTKVAINNGSLYL
                     SSGDDGIHAASEISVSTTGEVIIAKSYEGIESASITINSGKISITATNDGFNATKGTV
                     AGGTESNDGSRLYIHGGIIYLSVSNGDGIDSNGNIEMTAGTVLVHGPQSNPEVGVDYN
                     GTFNISGGLLVVSAPGSNMTQGPSTSSSQYSLKLITTSTNAANTLFHLEDAEGNDIIT
                     FKPARSYSSMIISSPALKNGSTYKIYIGGTDTGSESGGLYTGGTYTPGTLYQSFTVSS
                     TVTSIGSSSSGGPGGPGGRP"
     gene            complement(9535..10230)
                     /locus_tag="PK252_08645"
     CDS             complement(9535..10230)
                     /locus_tag="PK252_08645"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOT14815.1"
                     /translation="MKKGLFILFTVILWPVFLNSQSNDFGVWGNISLEKNTKKWDFAA
                     AAEVRAQSNASELNRSSIQLSTEYQLLKSLKLGASYQFMYFNDTKYNDFQPRHRGSLF
                     VQGRQKFGDFTIVLREKIQVTSKDESERIKKSGKIDTYKVNPEWTWRNRIKVAYNIPD
                     FPINPDISFETFYQLNNPEGNTFDKLRYTLTLNYKLSKQHSFELYELIDQDINVTSPV
                     TSLITGLGYTFCF"
     gene            10446..10519
                     /locus_tag="PK252_08650"
     tRNA            10446..10519
                     /locus_tag="PK252_08650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10480..10482,aa:Asn,seq:gtt)
     gene            11348..11704
                     /locus_tag="PK252_08655"
     CDS             11348..11704
                     /locus_tag="PK252_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14816.1"
                     /translation="MKLQLQKLVFVLLLFLNLLVLAVPFVYSYDLFNGVTNAKQIWFY
                     LALSLILLFMAIGYFIGYQGLSFGFIAVLSLLPYCKYGTAQIVYALFASKAILAKRRY
                     CKSIHKAVEEYGKKGR"
     gene            11952..12776
                     /locus_tag="PK252_08660"
     CDS             11952..12776
                     /locus_tag="PK252_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14817.1"
                     /translation="MKKNINTILAFKTIFIILFICIFFNSCDKEEIENESITVEYLSI
                     SNLNTKSQLTKAESDILKKAKERINKSLKFQNGKLSLAHVTAKDLNMSEDIFNLFQTI
                     VTFCNEAPHKKKVEVKSIKVKSASTENSGAVCDVIGDAIEACAGSDFEKECFRNYWDG
                     KGDKTLSYTIFIDIVKNVKISKTNNWIRVDINGKTYYQCQASFYDNSKYDYALGTCTM
                     YYDANGNAVGMKDTYDFNPENFGVRSSEAEVLTRTINTLGGACGAKNYEISYGIHK"
     gene            12895..13284
                     /locus_tag="PK252_08665"
     CDS             12895..13284
                     /locus_tag="PK252_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14818.1"
                     /translation="MKYDKIKWVIPILSLIVFVLWLKVIEVVSFSDKYDMKGLHYGII
                     MFSLVGAVFMLLGYILIPVTHFFLKKGILWVCVYIIMVCILPSLFATFLLSGNDMFIF
                     FFVCLKLFMGFNLPYFLLLIFVLKKSS"
     gene            13329..15131
                     /locus_tag="PK252_08670"
     CDS             13329..15131
                     /locus_tag="PK252_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT14819.1"
                     /translation="MNFVKNIAPIFVASVLLLPIFVLSNIFDGHIWGPFYFLVITGSI
                     FVIAISFYLYINKKILLNINLNIIELLMLFTFLYVIGSVVSCKFGLSYASNLYVFGIL
                     ILLIFIAKSWVFNTGKNWHIFFIVSIFMLIAIFEVVLGFLQLGRQYSKNQDLFKITGT
                     FDNSGIYANYLSIICVFALGLCLHVDLNMKKLRYLSFFTFFIIIVILPFTQARTSWLA
                     VLIGIITVSWPKIKTISIIKLMIANAKNKLLTFSFLFAVVFFIFYHLYQYKVQSAKGR
                     LLIYGVSLNMIMDKPVFGHGFNRFLAEYNIYQAEYFKNHSHTINAVLADNVRSGFNEF
                     IQVTVELGLAGLLLFIGFIVLLFYTKSKSEWEFIVIPAKGALSAVLICCLFSYPLRVL
                     PIAGCIVFILSIIMASNVDRGYNIRIGRQYYIPLALISTIIAILVCYIQWSDYNARKQ
                     WKQAIDYTQLNDFEKALPYYQKSYKILNYDGFFLYNYGAELIEINPVEGVRILEEATN
                     YLSDNDLYCYLGDGYRALKKYNKAEFNYQLSSLMVPCKFYPRYQLFKLYCETNQYCKA
                     RYIAQSIASMEVKVPSLIVLEIKSEIEEWLIMHK"
     gene            15529..16284
                     /locus_tag="PK252_08675"
     CDS             15529..16284
                     /locus_tag="PK252_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14820.1"
                     /translation="MEYLSVKKLFKSNLCSTFVLMLLAFIFIGLWSCEKEDSPNNNYS
                     EYLQLDSYELSSMTEKDMKIMGKALERLNIEYTNGVYFVKQKSGSEVNISDELFTFIK
                     NSFDYSNKLFKPNSVIYKKVRVKSGSPEGGKTEKDTTNCVAIAIAKMANLSSDSVIAI
                     CKRLFGNDGVPSDSIDYVVKYICPTAQKSTKYTPGFTNYDFIWFKTGPDSGHAVNATR
                     NFENGYIFFYDGQNKQNGIISSDRVETRYSIPK"
     gene            16296..17075
                     /locus_tag="PK252_08680"
     CDS             16296..17075
                     /locus_tag="PK252_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14821.1"
                     /translation="MNKLMSLIAFCFPGILFAQENLSYERKMSAYTDSVSKKFGVIFN
                     IPDKFTDLNIFYIPWKVRMDDAKYIGSVYGPIFLSNDKQCILMYPAQPWYVSKEDREI
                     YIKTAAINDIMNNTTTSVESQGTMNNSFPRSQITAEIKTALGLYYGYGHPLNNDSARM
                     DFNGYVTIVAGKKAREMFNADSIYIYDIPGGDSVCFLDESLEKMRKDIYLYCTGLFII
                     KKDRGTIDIKLLFTKEGKKREDEYINLLSKQIWYDDNFKHE"
     gene            17087..18658
                     /locus_tag="PK252_08685"
     CDS             17087..18658
                     /locus_tag="PK252_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT14822.1"
                     /translation="MRYKLSVFLVFLGSVFLTSEKFVDTENTIKFYFTILAALVGIII
                     VSFNSNNLKLELQKLTSFSILKGFYIAGVLQAIYGILQYIGKYSSNNEAFAITGSFEN
                     PAGFIAVLSFLFSIGVYWCIKSKKIEQRLIFFSLGLILYSIILTGSRTGIIAVFFSTF
                     TILSIEFQLLLKIKKLKYYRLIICSSIATVLFILFLLYKNKEDSANGRLLIWKVSTEM
                     IKDKPLFGFGYKGFQANYMDYQAKYFEKNPQSKFKQLADNVIHPFNEFVNIAISFGVI
                     GLALYLLVLTFIIWMLLNFEHQHKSILVGLFITFIIISCFSYLFCYSSIWILIGYFSL
                     VVFSKWIPYIRISLITKIAVIAFCLLGIIFFSSRIVWELKWKNILVKSLQGQTEQMLP
                     QYEKLYPFLKHNEYFLYNYGAELNIAKQYDKSIIILKECQKFYNDYDLQMLLADNYFQ
                     IGDTINAIRIYCCAENMIPCRFLPLYCQMEIYEKNGKKNKVNEIARKIVEKEIKVKSA
                     IVESIIEKAELNLSN"
     gene            18878..21517
                     /locus_tag="PK252_08690"
     CDS             18878..21517
                     /locus_tag="PK252_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14823.1"
                     /translation="MRKILLLLFVFALVACNKNKTSNVAIIKVNDIPDTCVILNKWQV
                     LGPFSSNDKEHFINEDNLQLFKYKESGISFLDFMRISSSRSLSSDTIRLDSCFQNKFV
                     FSGKIPLDFNEILKVNKEKLKGNVYCACLILSKKNISTRLHFSSTGAEKIWINGKLIC
                     STDYSKPLGSYTQFIPIKLKQGQNVLLVKVNKLYTDWQMYARLENESPTALKRYFGLH
                     NHFILYGSIMQFDSIVLDPIFPRCNGRLTMLDQNKNIVFSDTLLENKYWSHCVSTLKK
                     GIYQVRFSASNVTLVQDLYRGDIHDTIPKIIKELKLINLSGKNKDNIEALIFRYNYLL
                     TQTYPGDMKYVSLFMQLSDAFNALQKGKNPFNHTSGCFIRSYISDIDSSIQYYILHVP
                     STYKKNVPSSLAIAIPATVYGNLPYLKSFRVGNSKLIDFFQDMAEKYNMILIEPGSRR
                     YSRPNYNTIEEAELFNILNEVESDYNIDKNRIYLTGTCSGGNEALKLTVQFPDRFAAI
                     GLVAPEIIYPEESNNSAIMFIKNIINVPIFDTHSLIDRHIAVERSEVFNEIAEAFSFK
                     DFKYIRMPNEFPMYYPDDFFEDALEFCSKRSLNPSPKEIYFSTAKMLYNKSFWITINA
                     INYPDTAHIHAKMRWNTLTIEKSNVLDYSVDLRTLPYEKDKTLKIVDNGKVVFKGVIR
                     DSVLHIALKNKENRTLKSRSIAGPLSHVFVKKFIVVKGTTGSKEETTKISALADTIVK
                     YWMERYFVTCRVKKDTEITKKDIEDANLVLLGNPASNAIFNRLSDSLPVKISNSNIQL
                     FKKVIKGDKLCFYMVFPNPLNKSKYVALIGYNNPAYISLGSETGKFDDISDYGWYDYK
                     IWDEFSSSQGLEFGNFNQYWKLP"
     gene            complement(21636..22871)
                     /locus_tag="PK252_08695"
     CDS             complement(21636..22871)
                     /locus_tag="PK252_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT14824.1"
                     /translation="MAANVPERLKISTFKLNALLAITQAINENLPKDDLLKRYEQILK
                     EDLNIGKILIFKHNGVSWECILGSGIKDPKSYNINVDADLLKYSEITFVTSTSITSSS
                     ESFDIVIPVYNNSKPLAYVLIGDIEEEGEGVSPTIKHLHFIQTLSSIIIVAIENMRLF
                     QESLRQIAIKKELELASRMQSMLIPKNDELPKNEHIWITSFYHPHLDVGGDYYDFIRL
                     SENEVGFCIADVSGKGISAALLMSNFQANLRALFTSEISLPTLVEKLNERVLKSANGE
                     KFITLFVAKYNYINKELEYINAGHNHPILYETKSKNLVFLKEGCVGMGMLDEIPVIRK
                     GYIKVEQHTKLLCYTDGLVELMDDKEVEFGTTVLEQHISNNSDIKSNIDAIIEDQHIL
                     SGNKAIFDDITILGVEFYP"
     gene            complement(22981..24435)
                     /locus_tag="PK252_08700"
     CDS             complement(22981..24435)
                     /locus_tag="PK252_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14825.1"
                     /translation="MQKYSNKLSFKIGILIIATEVLILSVLGFYNSRKFIGEIENKIK
                     KQMEMPAKMMTEGSLRYEASQKKGTIEDIIGETVENCLILGTDGIVAYSSEPEYIDKP
                     KDSVQILNGFDSISKEIVNPYFKTIEKDGSLYYVNISSVRFQDGKCIGNLFILAKADK
                     IKQQKSQIILTFLIGTILCLIISTIIILYLFHRFITSKITELLTVLEKLKQGKISKKN
                     GQKISSDEIGMLWDALNEVNTNLTEIIKRILNSAKKLAGSSEQMKDISEEIADGANKQ
                     AASAEEVSSSMEEMIARIEQNADNSKKTENISFAAVASVKEIASESELSLQYIKDISA
                     KISMVNDIAFQTNLLALNAAVEAARAGDQGKGFSVVAAEVRRLAERSKIVADEINERA
                     INCVQITERGHHKMAKLISEIENTARLVQEITESSLEQKTGVLQINQAISELNDIIQQ
                     YNFSSEKLASDAKHLEDEAEELKNNIMLFSVEEA"
     gene            24602..25162
                     /locus_tag="PK252_08705"
     CDS             24602..25162
                     /locus_tag="PK252_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT14826.1"
                     /translation="MKLKFLIFVAILISGINLCYSQKYFTKTGKAYFMSHTDEIDIDG
                     TSKSVVSFLDTKTGEMVFGVNIKTFEFTLATAVEHFNETYMESHIYPKASFKGKVKDF
                     QKIDFSKPGKYDVVVEGDLTIHGVTKKVSEKGTLVIKDKKIIASSNFKVAIADYNIKV
                     PKIVVNRVAQIIDVKIDAEYEPYTGN"
     gene            25371..25676
                     /locus_tag="PK252_08710"
     CDS             25371..25676
                     /locus_tag="PK252_08710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT14827.1"
                     /translation="MNLGQVNISQLEYAAELLKALSHPLRIAILNLLLEEKELTVTQI
                     HSSLNIEQAVASHHLSILKSKDILVSKRRGNKTFYCIKNNNFIQLLACINNCSCKNK"
     gene            25676..28459
                     /gene="polA"
                     /locus_tag="PK252_08715"
     CDS             25676..28459
                     /gene="polA"
                     /locus_tag="PK252_08715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT14828.1"
                     /translation="MMSEKRLVLIDSYALIYRSYFAFMKRPMYNKQGFNTSTIFGFLL
                     TIDDIYRKLKPTHIAAAFDLGGKTFRHEMYEQYKANRQAQPEEIRKSVPFIKEILQAM
                     NIHILEAEGYEADDVIGTMAKLASAKDFETFMVTPDKDYCQLVCDKIKIYKPQRSGND
                     AEILDVEAVKTKYSLESPEQVIDMLALMGDSSDNIPGVKGVGEVSAKDLIVNFKNVEN
                     ILANLDKVKPKQRELIEASKEMLLLSRKLVTINTGAPITFDEEALLSKPFDDERLLKL
                     LQELDFKTLIPKFVKNAQPAQAAKPVSNTPDLFGFAIADAENTAAVETYKTIANVTHQ
                     YFWIEDEDSLNKLCLVLEQAQMFCFDTETTGLDPHTDELVGISISVKAHEAYFIRIAD
                     DFEKAKTFLSPLKKAFENANITKIGHNLKFDILFLLRYNITVAGPLFDTMIAHYLLYP
                     EQNHKMDTLSVKYLGYRPIPIEELIGRKGAAQKKMNQVEKGKLLDYAAEDADVTFQLY
                     GVLKEEIDKSFAKELFYNVESKLLEVLINVEKAGFTLDSAYLTRYKKQLAEDIKQLEQ
                     EIYNLAGQVFNIASPKQLGEILFEKLAIPYEGKLTKTKQYSTGEEVLVQLEGKHPIIA
                     KILEFRGLSKLLSTYVETLPDMINKYTGKIHTTFNQTLTVTGRLSSLNPNLQNIPIRE
                     ERGREVRRAFTCSEGNILLSADYSQIELRIMAHMSQDDNMIDAFLHDADIHTATASKV
                     FKIPVEQVTKEQRSKAKTANFGIIYGISAFGLAQRMNISRTEAMNLIQEYFKTFPGIK
                     QYMTKMVLFAKEHGYVDTILQRRRYLPDIHSQNAVVRGMAERNAINTPIQGSAADVIK
                     IAMINIFHKLQEEKLQSKMILQVHDELIFDVLPQELEKVRQIVVREMENAYPMAVPLK
                     VDTGTGQNWLEAH"
     gene            28478..29683
                     /locus_tag="PK252_08720"
     CDS             28478..29683
                     /locus_tag="PK252_08720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT14829.1"
                     /translation="MSSKILIADDSKLVVSLIKSLFAEHNDNYVVIEAVNGREAIEKA
                     LSEEPDVILMDWQMPEMSGIDALKTLKSDIRTKEIPVIMLTASEATTEAFECGASDFV
                     QKPFNKNELIARVNTTLDAVMARKELKKRLVDIEIEKDRLKLQKEVLIRQRKEMLQST
                     WMAAQVHRYLFANSELLHQMYPDSLYLSMPVSEVPATFFWTKVIQQNLYFCLIFHENT
                     GVQSVLYASIFKKFIEEYFQQNIMANIDLVELLKKLESSVEGFITNEDIAMPYTEIVF
                     CSLDYSSSTLNYAGVNTSLFALKRDKLVELKSERSVNGFFTKDLEIVPHKIQLAKQDI
                     LYLLNDGFLGFRKSYPDSYLSDELMELLRTVSKEDIKKQAKMLQKTFEHWKTDLKQQT
                     DMAVVGIRL"
     gene            29888..30649
                     /locus_tag="PK252_08725"
     CDS             29888..30649
                     /locus_tag="PK252_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14830.1"
                     /translation="MNPPNYGQTYAYKIKDSILLNDQKYWFKVFESSDSSYSEWKLNG
                     YIREENKIVLYSNQLWGIDTLYNFNKTVGDTIEFNHCNIKVSGISSEIIAGKNRKSYD
                     IINTHNTFDVKYYEGIGSNEGILAPYYFCATGACRSLVCFYKNNELLYLNPAFTTCYL
                     HSQLNNIVSTKEISFCIYPNPSTGIISISLNNNSSSSNFDFVIYNSLGTIVAKQSINT
                     AKTLINLSYLAKGVYYYRLNYQKNTYKIGILLITL"
     gene            30673..31905
                     /locus_tag="PK252_08730"
     CDS             30673..31905
                     /locus_tag="PK252_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14831.1"
                     /translation="MKTKILILALVGISAFFSCSKSDSDENSGNEENKTIKKQLNGFA
                     QKGPFVNGSSVMIFELDNSLDQTGKVYNTTIADNSGKFEKKNVELSSSFVLFKADGFY
                     FNEVANEISGSQLTLYAITDLSKDSAVNVNVLTHLERSRIEYLVLQEKKAFADAKKQA
                     QKEVLDIFNISKPDITSSEKLSLVKSGDDNAILLAVSVILQGKRTSAEMSELLANIIT
                     DIKADGILNDSTLGSKLIEDVKYMDLAAVRANLIKRYQELNESIEVPDFEKYVKSFIN
                     NTKFKPKKSITYPYRSKYGFNLLSDSVTTIDNKSNWDFAYSMSANVPDGYSLKIVLKG
                     GLWWYRVMPNGPVNWDVSSYDSGKEEQTFEVKKPNINSDISIQLPFGDTITIEYYENN
                     AVVPTKIKKVFCPETVTK"
     gene            complement(32128..>32583)
                     /locus_tag="PK252_08735"
     CDS             complement(32128..>32583)
                     /locus_tag="PK252_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14832.1"
                     /translation="NDIKTRMVKNEALPSFFVGYKYLDVSGSNGLHAWEAGIRVPIFS
                     GKTKDAKAQQRRITLHSEKTMLEMQTEIDELLARMNQCFDRINFYYDFGRQQARKYNE
                     AARQYIARSGKADITYLNLLVKREQYYFDYIEALNQYNQYAIRFEVYAY"
BASE COUNT        10513 a         5303 c         6123 g        10644 t
ORIGIN      
        1 actttttgcg gctttcagaa agggttggtg ctaaataccg acaaagtcgg gaaaatgatg
       61 tgtccaccat gcggttgaat ttttcctgct ccaatgctga tgaaatatac gaaggaattg
      121 ggcgtatggc cgaggcgctg aaagagatga tgtagctttt acaaaagctg cctcatcact
      181 atcatttgtg acttttcaaa tccgtatttt ttgtaaaaat ggatggcaac atcgttgttg
      241 taatcggtca gcagggtaat ccgcaagcag cctctttctt ttgcaaatgc aatggctttt
      301 tccaatagca tgctgccata acctgaagtt ctgtgctctt tatcaataat catgtcttcc
      361 aacacagcta cttttccgcc aagtgctgtg ctgattgaat acaggaggct aaccatgcca
      421 attaccttgt tatttttttt caataccaat atttctccta tattggggtt atcaattatc
      481 tgggtcaggc cggcagtttg cttttcatta tcggggacaa attccaggtc ttgcgaaaaa
      541 agctggttta gcaattcaca acaccgagct acttcgttaa gggagacttt ttcaataata
      601 acttgcattt tgggtgtaaa ttttagaaaa tgtactacaa aagaaatttt tttataaaaa
      661 tatgcccttt tttaaagcga atttttattt ttggagttag tattaatcta tccaacatat
      721 tttaaaattt tttggtggat aggtgttttt tttcatacaa ttacccccct tttaaaactt
      781 gaagtaaata ccaattatta atacattaaa tctcatactg atgaaaatat attttttatg
      841 ttttttgtgt ttgataacgt tattttctgt tgcagaagca aaagaaataa cgattaaaaa
      901 attgccagcc gggaattcta tttttattga tggcgatgat gacgattggc ctgcaacagc
      961 ggtatggcaa gcggtggacc aacacaaggg ggaacctgcc aataaaaata atacggtatc
     1021 cttaaagatg acttatgatg atgattttct ttatgttgca gtgaaagtgc tggatggaac
     1081 aatagacaca gctcaaagta ctaatgcttg ggaaaaagat tgtattgaag tttttttgta
     1141 ctaaacgata ctatctctga tggagcatcc tactctacac caatgtatgg ggcatggcaa
     1201 ttacgaaaag tatatggccg tcctatgaca gatctacctg gtaaaggatt tcaagcagaa
     1261 gagactgata ttgctggagg atacttgcaa gaatggaaat tgtcttggga taccttagct
     1321 tcaccttcgt acggagcata ttggggaggc aaaaaatttc gtttcgagat attaaatcaa
     1381 gacaatgatg gagctggctt aaatagtcag ttgttttgga attcaaatgc ctatgatcaa
     1441 tggaatacca taaaaaacca aggtactgtt tatttggatt attcgccctc tgatgattta
     1501 gtcttatcta catcttcgtt taatattagt gcctttttta aaatatttag tgttgaagta
     1561 tatactgctg atgcatggtc agcacaatca aataaatcat ggctcacttt ggaaaatact
     1621 tcaggagaag gtaacggtac tattaagttt tcggtttctt caaataacag tgaactgtca
     1681 cgtgaagcta aaataactgt tagcaatggc aaagtgtcaa aagttgtaat tgtaacgcag
     1741 gcgggagtct catgcagctc attattggca aaaaccaaac ttgagcttca ggaggccaga
     1801 gatagtatca atatgctttt agataaggtt gcacgatatg acagtattac tgttgctgtc
     1861 gttgaaatga aagattttac tgctatagtt aagcttccag agacaaatgt aagcctgaaa
     1921 ttatatccta atcctacaaa agacaaggtt ttggtagaat gtgaatcctt gatttccaaa
     1981 attgaaattt ttaatattaa aggtcaaaaa gtatatgaca aaatttcttc tgctaatacc
     2041 tcatatctta ggttagaggg ctttaaagca ggtacttaca ttttgaaagt acatactgat
     2101 aaaggcgttg ttggaggcat atttgtgata aaaagataaa cagtaatgct tgtaaagtaa
     2161 agctctcttg ttataaaatg agagggcttt tttcattttg ctcaaaaatc catcctgata
     2221 gacaaattca taaccacgaa aattgtaact tgaggcataa ttttgtttta gcgttaaaac
     2281 gttaatttta aatgttatga ataagcgata tttcaatttt aaaacaagca ttttcagtat
     2341 gcttgcattt gttctaatta tttcaactga gacaattgca aatgcacagt cttctgccgg
     2401 gtcaggagct tatgccacca aaaaataccg aaaccttttt gccgaaaatg gtcattcacc
     2461 ggattcaatc aacttgaaac tcgaaaaagc atttcagcag cttttctatg gagatagtgc
     2521 taagcgcctg tattttgttt cgggcaaaaa ccaaaatggt ttgttggcca atttcaggga
     2581 tgtttatagc aacgatgtac gtagcgaggg catgtcttat gctatgatga tagctgtaca
     2641 gatgaacaaa aaagaggagt ttgatgcttt atggaactgg gcaatgactt atatgtacgt
     2701 ccgcaaagag gggcaccctt caaaaggcta tttttgctgg tcgttgaagc ccaatggtac
     2761 tcccaacttt gaggtagccg cccctgatgg tgaagagtat tttgctatgt ctttgtattt
     2821 tgctgccaac cgctggggca atggacaggg tatttataat tacaaggcat acgctgatac
     2881 tatactttta accatgcgcc atcatccggt ggtaaaaggc cgcactatca atgaagaggt
     2941 gaaggtaggg cccatggtca atgaaaagta caatatgata ctgtttgctc ccgattcggg
     3001 caaaaaccat tttactgacc catcatatca ccttcctgct ttttatgagc tttgggcaat
     3061 gtggggccct gagcctgaaa ggactttttg gaaagctgct gcggatacca gcagggctta
     3121 tttccgcaaa gcacagcatc tcaaaaccgg gatgtcgtct gattatgcca attttgatgg
     3181 cactccgcac ccaattgctt ggaaccctca ttcgcaccac ttttcatggg attcgtggag
     3241 aactatgtca aactggtcag tggactggtc ttggtggcgt aaagatacca tgcagcaggt
     3301 tttgagcaac cgtatccagg ctttctttgc ttcacaaggc attgataagt ttggcagcca
     3361 gtttgagctt gatggtaaag tgataaatcc tcaacaccga aaagggctgg tggcgactgc
     3421 tgctgtagca agcttggcag ctacacatcc tattgctaaa gactttgttg aggctttttg
     3481 gaatatccca atccccgaag atcatggtga gcggtattgg gatggttcgt tgtacctatt
     3541 gagttttttg cattgtagtg gcaattttcg aatttgggaa ccaaaaaaag tggattaaca
     3601 ctgttgtttt aacaagctag acctttcagg ttttgaaaac ctggaaggtc tagcaactca
     3661 ttaaggcatc agagaggatg ccttttttcg tcaaagcatg tatatttgca ctaacctctt
     3721 gacctgcaca taaaaagtaa ttttgaaaac gacttttttc ttttttgctg gataataatg
     3781 ctgagttatt cttaaagtat ttatttgatc aatattacgc tgagctgtgt aatttatctt
     3841 tccgttaagt tggccgggca gagattgctg aagatgttgt gctggaagtt tttatcaatt
     3901 tttgggacaa acgactggaa ataagttatg aaggccttgt aaaacctttt tttatccggt
     3961 caattgtcct aaaaaagagt ccatctgtca ttgaagaaat tgggttattc ccctttttgg
     4021 ctatttttgt caaaatagtt gttaatcatt taattacagc aataatgcgt taattgttcc
     4081 gttgatatat acctcaatta caattaatca taaaaacaaa aaaattttta tctccattgg
     4141 gggtataaaa aaagttgggt gtcatagtac cgaaaacgga aaaaggaaga tttttgaaaa
     4201 tgttcacgca acgcttttgc tggttcatcg tcataaatta cctgtttacc gactaatatt
     4261 agtttgacat aatgatagaa aatatttttg atgcacattt ttaagaaaca ggatgaaaaa
     4321 cgaaattttt aaaattaaaa acaagacttt gcaattagtt tcaatcagtt tgattctttt
     4381 ttgcccattt tctcttaaag cggcagaacc atcccctgct cccggatctg ctgctgctga
     4441 agggaaactg aaatctgtaa gcggctatat taaagatgcc agtgatggcg aggccttaat
     4501 tggggctact gtacttgtca gggaactgaa aaagggagct gctgccaata cttatgggtt
     4561 ttatagtgtc agccttttgc caggtaagta cacgctggat ttttcttacg ttggatacaa
     4621 aaccgaaacc aaaaccattg aactaaagga aaatacaact ttgaacattg agctttccaa
     4681 tgaaacaaag gaaataaatg aagttgtagt aaaagcagaa agtaccaacg ataagttaaa
     4741 acgtcccgag atgagcgttc aaaagcttga gatgaaaacc atcaaaaaag tgcctgcgtt
     4801 gatgggcgaa gtggacgtta tcaaagttat tcagatgttg ccgggtgttg tttcaaccag
     4861 cgaagggtca tcaggcttta gcgtccgtgg gggaggtatc gaccagaacc tcattatcct
     4921 tgacgaagct actgtttaca atgcttcaca cttgatgggt ttcttctcgg tatttaataa
     4981 tgatgctatt agcaatgtaa aactttacaa aggtgatatt cctgcaaact atggaggccg
     5041 tctttcatca gtacttgatg tacgcatgaa agaagggaac atgaaaaagt ggtctgcagc
     5101 cggaggtttg gggttgatag ccagccgtct gactatcgaa gggcctattg ctaaagacaa
     5161 ggctgccatc ctgatctcgg gaaggcgcac ttatgctgac ttgtttttcc cgcttgcaac
     5221 agataagaac cttaagaagt caaaacttta tttttacgat cttaatttga aagggaatgc
     5281 catcatcaac caaaataacc gtgtatttgt ttcgggttat ttaggccgcg atatttttgg
     5341 tcagaaagga gccggggata taggttttgg caacaagaca gctacggtac gctggaacca
     5401 ccagtattct ggcaaattat tctcaaacct taccggtatt gcttcaactt atgattatga
     5461 cttgttccaa tctctggaca gtaccgaata ttcatggaaa tcgaacctga cagactatgg
     5521 tttaaagctc gatttcaact ggtttgccaa tacagataat gaaataaaat ttggggtatc
     5581 ttcaacatat cataccatta ccccttgtga tgcttatagc aaatcgagta ctgtaatgca
     5641 atacttccca aagaagactt caaattctta tgagcatggt atttatgcta ccaatcaaca
     5701 aaaaattggc gataaattga ccctgaaata cggaatccgt tattcaattt tccaaaatgt
     5761 tggaaagggc attattgacc gttttgacga taatcataat tatatcaaat cagatacatt
     5821 taaagccggg aaattgatta atacttatac aggatgggag cctcgtttag gcgtaaactt
     5881 taccatcaat gatgtttcat ctgtcaaagc aagctattcg cgtacctacc agtatttgca
     5941 acttgcttca aactctaatg gtggcatgcc gcttgattac tggttccctg caagcccaaa
     6001 tgtgaagcct caggaatctg accaatacgg cttaggttat ttccataatt tgtttgacaa
     6061 tacccttgaa acttctgttg aatttttcta taaagatatg agaaacgtaa tagatttccg
     6121 cgaccatgca gacctgttgt ttaatgaccg tttagaaggt gatgtcagga ctgggaaagg
     6181 atatagttac ggtgccgaag tccttatccg caaaaatgaa ggggccttta ctggttggat
     6241 cagttatact tactcaaggg cattcagaaa agtggctgct attaatgaag gaaaagaata
     6301 ccaagccccg tatgataagc ctcataatgt caatttggta ttgaactatg aatttacgcc
     6361 acgtatcagc gcttctgcca attttgttta cgctaccggt acaccgatga ctaagccctt
     6421 gggtcttatg aaattgatga caatgtagtg tttatatact cagaccgcaa ttcgtcccgt
     6481 atgcgtgatt accaccgtct cgacttgtca ttgaccatca aagacaaggg tgcacctgac
     6541 aggttttggc atggcgagtg ggtattttcg gtatacaacg tatatgggcg ccacaatgac
     6601 tggatgatca attatcaaaa caaatttgaa ggtacatctt ctgaagtaaa agaggcaaca
     6661 cgtacttatc tgccattcct tttctttcct ggtataactt ataatttcta tttctaaaag
     6721 acgatgaata cgatgaaaaa aataaaaaac aaattgaaat tagccttgta tatcatacct
     6781 gctattttca tcactgcatg tgaagaacca attgaagaca agcttgattc gacttatacc
     6841 cggttggtgg ttgatggtac tattaccaca gacaccatgg cccataaagt acttttgaaa
     6901 tattcaaatg atgcattgaa cagaaatcct atccgatttg tatcaggtgc agttgtaaca
     6961 atttctgatg ggactaatac attcaacctg cacgagaacc ctaaagtccc gggagaatat
     7021 cttacagaca gcaatgtata tggagttgcg gggaatacat atacactgaa tatttctaat
     7081 gtggatgttg atgataacgg ggtaaaagaa acttatacag cttccagtta tttgccttat
     7141 atgtcaagaa ttgataccat tactgtaact gattatatcc ataacgataa attacgaggt
     7201 tggcttatca atttatatgg ccaagacagg ggcggaagaa attattacct taccaaagct
     7261 atcagaaatg acacattggt tactgatagc ctcaaggaat atggggcggc agctaattca
     7321 ggttttgaag gtgaaaaata tgaaggtttt gctgtttatt ttttagaaaa agatgataca
     7381 acggatattg tcagggagaa tgacagaata acattagaac ttaactgtat cacaaaagat
     7441 tattatgatt ttgtgattgg ttttcagaaa gaatattttc ctaaaaaccc tatatttagc
     7501 ggtccatctg caaatgtcac cacaaatatt tctccaaaag aaaaagctgt aggttttttt
     7561 gcggcatact cagtcgtcag gaaatcaaca atatataaga aaagaaataa aaaatacgat
     7621 taattggttt gttcgtatgt cccgacaaag gtcgggattg ttcattggtt taggtttcgg
     7681 aaagcagcct tttgagctgc tttccttttt tcccttatta acgaacatta attttattta
     7741 tgctttacgg cctcccacct ggtcccccgg ggccgccaga tgaactgctt ccaatagaag
     7801 tgacagtgct tgatacagta aagctttgat atagtgttcc cggagtatat gtcccgcctg
     7861 tgtacaatcc tccggactct gaacctgtat cggttccgcc aatataaatt ttgtaagtcg
     7921 aaccattttt taatgcagga gaagaaataa tcatcgacga ataactgcgg gcaggtttaa
     7981 aggttataat gtcattgcct tcagcatctt cgaggtgaaa cagggtattt gctgcattgg
     8041 tagatgtagt gataagtttc aatgaatatt gcgaagaact ggtactcgga ccttgcgtca
     8101 tatttgaacc gggggcagag actaccagca atcctcccga tatgttgaaa gtgccgttat
     8161 aatctactcc aacttcagga tttgattgag gcccatgaac taatactgta cctgctgtca
     8221 tttcaatatt tccattgcta tctatgccat ctccattaga tacgctcaga taaatgatac
     8281 ctccatgaat gtacaaacgg cttccatcat ttgattcggt accacctgcc actgtacctt
     8341 ttgtggcgtt aaacccatca ttggtagcag taatgctgat tttcccgctg ttaattgtaa
     8401 tagaagcgct ttcaatacct tcgtatgact ttgcaattat tacttctcct gttgtcgaaa
     8461 cagatatttc ggatgctgca tgtatgccgt catctcctga agaaagataa agagagccat
     8521 tatttatagc aacttttgtg tttgaatgta cagcatcatc tgttgaggtg atattgaata
     8581 tgccaccatt gatttcgacc aaattagtac ctttaagccc tttggcagaa attgaattgg
     8641 cattgcctga agttgtaata ttaaaagtac catctgtaat aacaatgtct ttttcggcag
     8701 aaataccatc agctacagca ttaatgttaa atataccatt tgtaatattg acaaaaccac
     8761 attgtgcatc gcttgtattg tctgatttta ttccatcgcc accagactta atggtgaaat
     8821 tgccattatt tattaccagg taatcttttc ctcttattcc atcatctatg gctgttactg
     8881 tataagtgcc acttttgata attaacccgt ctttactcga aataccatct ttatagtttg
     8941 ctttaacagt taaagaacca gttccaaaaa gcgtcatatt ggttttactg aaaagtgctg
     9001 catttggttc atcttcatct gcatttgcca aaatataaga agctccatcg gtaagtatat
     9061 tgtttgtatt ttctgacagc atgataagga ctttgcttgc tttctgcaca aaaataggtg
     9121 cactgttcga gcaggttatg tcagcaccat taaggataat acgtactaca tcatctttag
     9181 tagcactaac aataagctgt ccatcagtta atgaacctga aaccttataa gtaccggcag
     9241 tagtaatggt aacaacgctt ccgttaattg ttacaccttt agcgtctgta gtagcagaac
     9301 tgccatttaa aacaatattt aattcactgg cagtatcccg gacataatct gttgaatctt
     9361 cttgtactcc tgtaaatgtt gtagttttac tttgatcgtc actttgggtt tcgctatctt
     9421 ggtctttttc gcaagcagca aatgccataa gcactatcat aattgaaatg aaaaaagaaa
     9481 tgtgttttaa aacctgggtt ttcatagtat caatttattt aaaagatagt aattttaaaa
     9541 acaaaatgta tatcccaagc ctgtaattaa actggtgaca ggcgaagtaa catttatatc
     9601 ctgatcaatt aactcataaa gctcgaagct gtgctgtttt gataatttat aattcagcgt
     9661 gagcgtataa cgaagtttat cgaaagtatt tccctcggga ttgtttagtt ggtaaaaagt
     9721 ttcgaacgaa atatctggat taataggaaa atctgggata ttatatgcta cttttatgcg
     9781 gttacgccag gtccactcgg gattaacttt gtaggtatct attttgccac ttttcttaat
     9841 acgttcgctt tcatcttttg aagttacttg tatcttctca cgtaagacaa tggtaaaatc
     9901 tccaaatttt tgacggcctt gaacaaaaag gcttcctctg tgtcgtggct gaaaatcatt
     9961 gtatttagta tcattaaaat acataaactg ataagaagcc cctaatttca atgatttcag
    10021 aagctgatat tctgttgata actgtattga cgaccgatta agctcgcttg cattggattg
    10081 tgcccgtact tcggcagccg cagcaaaatc ccattttttc gtattttttt ctaaggagat
    10141 attgccccag accccaaaat catttgattg agaatttaaa aaaactggcc agagtattac
    10201 cgtaaacaga ataaataatc cttttttcat cagacaaatc aattaattag gtatcagtta
    10261 ttttagtatt aatacatgaa tatgaaacta taccctaata aagaaaaaat gggggaatag
    10321 gacttatctc attgacgttc tttaatttat attctacagt acgaaaaaag aaatttttat
    10381 tcaaaaaatg tgtgtaatta aacaaatgct attacttttg cagcacaaaa tttcagcgat
    10441 gaaattcctc cttagctcag ttggttagag catctgactg ttaatcagag ggtctcaggt
    10501 tcaagtcctg aagggggagc agataaaagc cggaacgcca atatttaaaa tggtttccgg
    10561 ctttcttatt tttctactgc cagaataact aacatttctt tcatgatcta tctaagtcag
    10621 gagattgaaa tcatgggctt tggttttttc cgaatttgtt ccgtattaca tttaaatttt
    10681 attcattgtg tttattgata ttataaaaat ttgccgcatc agtgtaccaa aattcacctt
    10741 tgttatgagc aagagaagta aaatcaaatt ttataagtaa tactgcaagt gtaagaactc
    10801 aaattttaga tgatatagat ataatttcac aacctggtca acttacggtt caacgaatag
    10861 agtggcattg ttttgaagga gttgatgcag atgttgtgaa ttttgtaaaa ccagaactca
    10921 ctaaacgtgc aataccatta ggctttgtag atgaaatttt tacagacaga gactattatt
    10981 acattcttga ttcgcaacaa aaggccattt ttatttttga taagcaaggc aaatttgttg
    11041 acaataattc tttgcattta aaaaatcgtt cactttttgt ttaaaaaatg caagaaataa
    11101 aaaatgaaag aaatttaata acgtttttac aagctttatt ttgttgttgt attcatattt
    11161 gtttttgtaa ggtttagctt ttttgttact acttggtaac gaagtataaa aagtatttct
    11221 gttggtttat tttgtgcagt tggttgaagg ggagatttta gagtaatagc cctaaagttg
    11281 gactttaata ttgttttgcc ggagatgaat gccgggagaa acagatgatg tttatttgcc
    11341 ccaaactatg aaactgcaat tgcaaaagct tgtatttgta ttactgttgt ttttaaacct
    11401 gttggtgtta gcagttccat ttgtatattc ctatgacttg ttcaatggcg ttacaaatgc
    11461 caaacagata tggttttatt tagcgctctc gctgatattg ctctttatgg caattggcta
    11521 ttttattgga tatcagggat tatcatttgg gtttattgct gttttgtctc ttttgccata
    11581 ctgcaagtat ggtacagcac aaattgtata tgcactattt gctagcaaag ctatactggc
    11641 aaaaaggcga tattgcaaaa gcatacataa ggctgtggaa gagtatggca aaaaaggaag
    11701 gtagaaagca tggacgaaac taaaagccga gatgaaacaa ttgacagata atatattaaa
    11761 cgggaaaaag gggaagcggg agctttgtaa gctgaaaaat aacaactccc actctccccg
    11821 gacatcccac ctaaacaatg aaaggaggtg acaggcaaag gtatgtataa ataaaaccca
    11881 aaacaccaca tttcaagata tttgtgatgt attttattga aaatgatttt attaatctta
    11941 aattttaaaa aatgaaaaag aacataaata cgattcttgc atttaaaact atttttatta
    12001 ttctatttat atgcattttt tttaatagtt gtgataagga agaaattgag aatgaaagta
    12061 taacagttga atatttgtct ataagtaatt taaatactaa atcacaattg acaaaagctg
    12121 aaagtgatat tttgaaaaaa gcaaaagaaa gaattaataa atctttaaaa ttccaaaatg
    12181 ggaaattaag tcttgctcat gtaacagcta aagacttgaa tatgagtgaa gatattttta
    12241 atttatttca aacaattgtt actttttgta atgaagcacc tcacaaaaaa aaggttgaag
    12301 ttaaatcaat aaaagtaaaa agtgcatcca ctgaaaatag cggagctgtg tgtgacgtaa
    12361 ttggagatgc tatagaagca tgtgctggtt ctgattttga gaaagaatgt tttcgtaatt
    12421 attgggatgg caaaggagat aaaacactgt catatactat atttatcgat attgtgaaaa
    12481 atgtaaaaat ttcaaaaaca aataattgga tacgagttga tattaatggt aagacttatt
    12541 atcaatgtca agctagtttt tatgataatt ctaaatatga ttatgcatta ggtacatgca
    12601 cgatgtatta cgatgcgaat ggaaacgcag taggaatgaa agatacatac gattttaatc
    12661 ctgaaaattt tggagtaaga agctctgaag cagaagttct taccagaaca attaatacat
    12721 tgggaggagc ttgtggagcc aaaaattatg aaataagcta tgggattcat aagtaattaa
    12781 ccattataat gtttagctct tgttgatatt attcaagtgt aaattttgat attcttcaac
    12841 agggctaatc tatcaatgat tggatatgag atattgattt aataatttta taatatgaaa
    12901 tacgataaaa taaaatgggt tataccaatt ttaagtttga ttgtttttgt gctatggctg
    12961 aaggtaattg aagttgtttc gtttagtgat aaatatgaca tgaaaggact acattatgga
    13021 ataatcatgt tttcattagt aggagctgtt tttatgttat taggatatat tctaattcct
    13081 gtgacacatt tttttttaaa aaaaggtatt ctatgggtat gtgtttatat aataatggtt
    13141 tgtattttac cgtcattatt tgcaactttt ttgcttagcg gaaatgatat gttcattttt
    13201 ttctttgttt gtttaaagtt attcatgggg tttaatttgc cttattttct tttgttaatt
    13261 tttgttttaa aaaagagtag ttaaaagcgg ttttagcaag aatttttaat agccaatgtt
    13321 caagtattat gaattttgta aaaaacatcg ctccaatatt tgttgcatct gttttattgt
    13381 tacctatttt cgttttgagt aatatatttg atggtcatat atgggggcca ttctattttt
    13441 tggtaattac gggttctata tttgtaatag caatatcttt ttatttatat ataaataaaa
    13501 agatattgct caacattaat ttaaatatta tagaattatt aatgttgttt acatttttgt
    13561 atgtaattgg tagtgttgtt tcatgtaagt ttggcttgag ctatgcctca aatttatatg
    13621 tatttggaat tctcatcttg ttaattttta ttgcaaagtc atgggtgttc aatactggca
    13681 agaactggca catttttttc attgtttcaa tttttatgtt aattgccata tttgaagttg
    13741 ttttaggatt tttacaatta ggaaggcaat attctaaaaa tcaagattta tttaaaataa
    13801 cgggtacgtt tgataattct gggatatatg ctaattattt gtcaataata tgcgtatttg
    13861 ctctaggttt atgtttacat gttgatttaa atatgaaaaa gttacgttac ctatcttttt
    13921 ttactttctt tattataata gtgattttgc cttttactca agctcgcaca tcatggttag
    13981 ctgttttaat tggaataata acagtaagtt ggcctaaaat aaaaactatt tctataatta
    14041 aattaatgat tgccaatgca aaaaataaat tgttaacgtt tagtttttta tttgcagttg
    14101 tttttttcat tttttatcat ctttatcagt acaaagtgca atctgccaaa ggtcggctgt
    14161 taatttacgg agtgagcctt aatatgataa tggataagcc tgtctttgga catgggttta
    14221 acagattttt ggctgaatat aatatttatc aggctgaata tttcaaaaat cattctcata
    14281 ctattaatgc agtattggca gacaatgtaa gatcaggttt taatgaattt attcaagtaa
    14341 ctgtggagct tgggttagca ggtttattat tatttattgg tttcattgtt ttactttttt
    14401 acacaaaatc taaatcggaa tgggaattta tagttattcc tgcaaaaggg gctttatcag
    14461 cagtattgat atgttgttta ttttcttatc ctttaagggt gttgccaata gcgggttgta
    14521 ttgtttttat tttatccatt ataatggcat caaatgtaga tagaggatat aatattagaa
    14581 ttggcaggca gtattatata ccattagcat tgattagtac tattatagcg attttagttt
    14641 gttacataca gtggagcgat tataatgcgc gaaaacaatg gaagcaagcg attgattaca
    14701 ctcagttgaa tgattttgaa aaagctttgc catattatca aaaatcatat aaaatactaa
    14761 attatgatgg tttcttttta tataattatg gtgctgagtt aatagaaatt aatcccgtag
    14821 aaggggtaag aatattggaa gaagctacaa attatttaag tgataacgat ttatattgct
    14881 atcttggaga tggttatcga gctttaaaaa aatacaataa agcagaattc aattatcaat
    14941 tgtcgtcgtt gatggtccca tgtaaatttt atccacgata tcaattgttt aaattgtatt
    15001 gtgaaactaa tcaatattgc aaagcccgtt atatagccca aagcattgca agtatggaag
    15061 taaaagtacc atctttaatt gtacttgaga ttaaatctga gatagaagaa tggttgataa
    15121 tgcataaata atagacttgt aagtaatagg attgatattt atagtggtac tgcacaaggt
    15181 gaaccagcat tatatgctag caaagctata ctggcaaaaa ggcgatattg caaaagcata
    15241 cataaggctg tggaagagta tggcaaaaaa ggaaggtaga aagcatggac gaaactaaaa
    15301 gccgagatga aacaattgac agataatata ttaaacggga aaaaggggaa gcgggagctt
    15361 tgtaagctga aaaataacaa ctcccactct ccccggacat cccacctaaa caatgaaagg
    15421 aggtgacagg caaaggtaag gataaataaa acccaaaaca ccacatttca aggtatttgt
    15481 gatgtatttt attgaaaatg attttattaa tcttaatttt tttaaaaaat ggaatattta
    15541 agtgttaaaa aactttttaa gtcaaattta tgctcaacat ttgtgctaat gctgttggcc
    15601 tttattttta ttggattatg gagctgtgaa aaagaagact ctcctaataa taattattct
    15661 gaatatcttc aactagattc ttatgagtta tcaagtatga ctgaaaaaga tatgaagatt
    15721 atgggaaaag cattggaaag gctgaatata gaatatacta atggtgttta ttttgttaaa
    15781 caaaagtctg gatcagaagt gaatatttca gatgaattgt tcacttttat aaaaaactca
    15841 tttgattatt caaataaact ctttaagcct aattcagtta tttataagaa agtgcgcgta
    15901 aagtctggaa gtccagaagg aggtaaaacc gaaaaggata caacgaattg tgttgcaatt
    15961 gctattgcaa aaatggctaa tctcagtagt gattcagtta ttgcaatttg taaaagatta
    16021 ttcgggaatg atggagtacc atccgatagt attgattatg ttgtaaagta tatttgtcct
    16081 actgcccaaa aatcaacaaa gtatacccca ggctttacaa attatgattt tatatggttt
    16141 aaaaccgggc ctgatagtgg acatgccgtt aatgcgactc gtaattttga aaatggatat
    16201 atattttttt atgatggtca aaataaacaa aatggaatta ttagttctga tagagtagaa
    16261 actagatatt ctatacctaa ataaaaataa ttgttatgaa taaattgatg tctttaattg
    16321 cattttgttt tccaggcatt ctttttgcgc aggagaacct ttcgtatgaa agaaaaatgt
    16381 cagcttacac tgactctgta tcaaaaaagt ttggcgttat ctttaatatt cctgacaaat
    16441 ttactgattt aaacatattt tatataccat ggaaggttcg gatggatgat gctaaatata
    16501 taggtagtgt gtatggcccg atatttttat caaatgacaa acaatgtatt ttgatgtatc
    16561 cagcacaacc ctggtatgtt tcaaaagaag atagggagat ttacataaaa acagcagcca
    16621 taaatgatat aatgaataat actacaacgt cggttgagtc acaaggtact atgaataatt
    16681 cgtttccacg gagtcagatt actgccgaga ttaaaacagc ccttggtttg tattacggtt
    16741 atggacaccc tttaaataat gattcagcaa gaatggattt taatggctat gtaactattg
    16801 ttgcagggaa aaaagcacgc gaaatgttta atgcagattc catttatatt tatgacattc
    16861 ctggcgggga ttcagtttgt tttttggacg aatcattaga aaaaatgcgt aaggacatat
    16921 acctgtactg tacaggcttg tttatcatta aaaaagatag gggtactata gatattaagc
    16981 ttctttttac aaaagaaggc aagaaaagag aagatgaata tatcaacttg ctgagtaaac
    17041 agatttggta tgacgacaat tttaaacatg aataaaaggt agcaatatga gatacaagct
    17101 atctgttttc ttagtttttc tcggaagtgt gtttctcact tccgagaaat ttgttgatac
    17161 tgaaaatact attaagttct attttacaat acttgctgca ttagttggga taattatagt
    17221 gagctttaat agtaataatc taaaattaga attacagaaa ctaacctcct tctcaattct
    17281 gaaaggattc tatattgcag gggtattaca ggctatatat ggaattttac aatatattgg
    17341 caaatattcg tctaataatg aggcttttgc aattacaggt agttttgaga atcctgcagg
    17401 ctttatagct gtattgtctt ttcttttttc aataggggtg tactggtgta tcaaatcaaa
    17461 aaaaatagag caacgtttaa tattcttttc tttgggatta attctttatt ctattatttt
    17521 gacaggttca cgaacaggta ttattgctgt ttttttctca acatttacaa tccttagtat
    17581 agaatttcaa ttattattaa aaatcaagaa attgaaatat tatagactca ttatatgttc
    17641 ttcaattgcg actgttttat ttatattatt tttattatac aagaataaag aagattctgc
    17701 taatggcaga ttacttatat ggaaagtatc tactgagatg ataaaagata aaccattatt
    17761 tggctttggg tacaaaggat ttcaagcaaa ttatatggat tatcaggcaa aatattttga
    17821 aaagaatcca caatcaaaat ttaagcagtt agcagataac gttatacatc cttttaatga
    17881 atttgtcaat attgctataa gttttggagt aattggctta gcattgtatt tacttgtgct
    17941 tacttttata atatggatgt tactaaattt tgaacatcag cataaaagta ttttggttgg
    18001 attatttatt acttttatta ttatttcctg cttttcatat ttattttgct actcttcaat
    18061 ttggatatta attggttatt tttctttagt ggtattttct aaatggatac catacataag
    18121 aatctcttta attacaaaaa ttgcagttat tgctttttgt ttattaggta ttatcttttt
    18181 ctcatctcgt attgtatggg aattaaaatg gaaaaatatt ttggtaaaat cgctacaagg
    18241 acaaacagaa caaatgcttc ctcagtatga aaaattatat ccatttttaa aacataatga
    18301 atactttctt tataattatg gtgcagaact taatattgca aaacaatatg ataaaagcat
    18361 aatcatactt aaagaatgtc aaaagtttta caatgattat gatttacaaa tgttattggc
    18421 ggataattat tttcaaattg gagacactat aaatgctatt agaatatatt gttgtgctga
    18481 aaatatgata ccttgtcgat ttttaccgct ttattgtcaa atggaaattt atgaaaagaa
    18541 tggtaaaaaa aacaaagtaa atgaaatcgc aagaaagatt gttgaaaagg agattaaagt
    18601 aaaatcggca attgttgagt ccataattga aaaagctgaa ttaaacttat caaattaggc
    18661 tcttaatcaa taatacaagt ttctcgcatt taaccgctga tctgttgtgg cgaagctaag
    18721 aatttaaacg cagagatctg taagatctgt aacctccttt gtggttctct gcgttaattt
    18781 tctgtgttct tcgcggtttt atatattact taaaagtcta tttcgacaaa acttttaatt
    18841 ttcgaaaata aaagtgtcat tcttaacata agataaaatg agaaaaatat tacttctttt
    18901 atttgttttt gccttagttg cttgcaataa gaataaaaca tctaatgttg caataataaa
    18961 agtcaatgac atacctgata cttgtgtgat tttgaacaaa tggcaggtgt taggcccctt
    19021 ttcatccaac gataaagagc attttataaa tgaggataac ctgcaattgt ttaaatataa
    19081 agaatctggc atttcttttc tggattttat gagaataagc agtagcagaa gtctttcttc
    19141 ggatacaata agattggatt cctgttttca aaacaaattt gtgttttcag gaaaaattcc
    19201 tcttgatttt aatgaaatat tgaaggttaa caaagaaaaa ctcaaaggca atgtatattg
    19261 tgcttgtttg attttgagta agaaaaacat cagtacaagg ttacattttt caagtactgg
    19321 tgcagagaaa atatggataa atgggaaatt gatatgttca acagattatt caaagcctct
    19381 gggcagctat acacaattta taccaataaa acttaagcaa ggccaaaatg tattgctagt
    19441 caaggtcaac aagttatata cggactggca aatgtatgcc cggttagaaa atgaatctcc
    19501 tacagcccta aagagatatt ttgggcttca caaccatttt attctatatg gcagcataat
    19561 gcagtttgac agcattgtat tagatccaat attccctcgt tgtaatgggc ggttaacaat
    19621 gctagatcag aacaaaaaca ttgttttctc tgatacttta ttggaaaata aatattggag
    19681 ccattgtgta agcactttga aaaagggaat ttaccaagtt aggtttagtg cctcaaatgt
    19741 tactttggtt caggatttgt accggggaga tatacatgat actataccaa aaattataaa
    19801 ggaattaaag ctgattaatc tatctgggaa aaacaaggac aatattgaag ctttgatttt
    19861 caggtataat tacttactta ctcaaactta tccgggcgat atgaaatacg tttcattatt
    19921 tatgcaattg agtgatgctt ttaatgcctt gcaaaaaggc aaaaatccat tcaatcatac
    19981 atcgggatgt tttatcaggt cttatatttc agatattgac agtagtatcc agtattatat
    20041 tttgcatgtt ccttcaacct acaaaaaaaa tgtaccttct tccttagcaa tagctatacc
    20101 tgcaacagta tatggaaatt tgccatatct aaaaagtttt agggttggaa attctaaact
    20161 tatagatttc tttcaggata tggcagagaa gtataatatg atattgatag aacctggctc
    20221 cagaaggtat agccgcccta attataatac cattgaggaa gcagagctct ttaatatatt
    20281 aaatgaggtt gaaagtgatt ataatattga taaaaacagg atatacctta ctggaacttg
    20341 cagtgggggc aacgaagctt taaaattgac ggttcaattt cctgaccgtt ttgctgctat
    20401 tgggctggtt gcccctgaaa ttatatatcc tgaagagagt aataattcgg ctattatgtt
    20461 tattaaaaat ataataaatg tgcccatatt tgatacacat agcttaattg accggcatat
    20521 tgcagttgaa aggagtgaag tgtttaatga aattgcagaa gcttttagct ttaaagactt
    20581 caaatatatt aggatgccca atgaatttcc aatgtattat ccagacgatt tttttgaaga
    20641 tgccctggag ttttgcagca agcgttcact aaatcccagt ccaaaggaaa tttatttttc
    20701 tacagccaaa atgttgtaca ataaatcatt ttggataacc attaatgcta taaattaccc
    20761 tgatactgca catatacacg caaaaatgag atggaataca ttgacaattg agaaaagtaa
    20821 tgtcttggat tattctgtgg atttaaggac tctgccttat gagaaagata agacactaaa
    20881 gattgttgat aacggaaaag ttgtatttaa aggagtaatc cgggattctg tattgcatat
    20941 tgcattaaag aataaggaga ataggacatt aaaaagcagg tcaattgcag ggccattgtc
    21001 tcatgtattt gtcaaaaaat ttattgttgt aaaaggaact actggtagta aagaagaaac
    21061 aactaagatt agtgctttgg ctgatacaat tgttaagtat tggatggaaa gatattttgt
    21121 tacttgcagg gtaaagaaag atactgaaat tacaaaaaag gatattgagg acgctaactt
    21181 ggttttattg gggaatcctg cttcaaacgc tatttttaat aggcttagcg attccttgcc
    21241 agttaaaata agcaactcaa atatacagtt gtttaaaaaa gtaataaaag gagataagct
    21301 ttgtttctat atggtatttc ccaatccttt gaataaaagt aaatacgttg ctctgatagg
    21361 ttataacaat ccggcatata tttcattagg ttctgaaact ggcaaatttg atgatatatc
    21421 ggattatggt tggtatgatt acaaaatttg ggacgaattt tcttcttctc aagggttaga
    21481 atttggtaat tttaatcaat attggaaatt accctgattg gttctgctcg tttgttcgac
    21541 agccaaatgc agagctttag ccaaacaaac tggcaatgtg tgccgaactg gagttacaaa
    21601 aaaaacggca ttgtctggtt tttagtaata aacccttatg gataaaactc aacgcctaaa
    21661 atagtaatat catcaaagat agctttgttg ccggatagta tatgttggtc ttcaatgatg
    21721 gcatcaatat tagatttgat gtcagaattg tttgaaatgt gctgttccag cacagttgtg
    21781 ccaaactcca cttccttgtc gtccataagc tctaccaaac catcggtata acacaggagc
    21841 ttggtgtgtt gctctacttt gatataacct ttacgaatga ccggtatttc gtcaagcatg
    21901 cccatgccta cacacccttc tttgagaaac accaggtttt tgcttttagt ttcgtacaat
    21961 atagggtggt tgtgcccggc attgatgtat tccagctctt tgttgatata attgtatttg
    22021 gccacaaaca aggtaataaa tttttcgcca ttggcgcttt tcagcactcg ttcatttagc
    22081 ttttcgacca atgtaggcaa agatatttca ctggtaaaaa gcgcccttag gttggcctga
    22141 aagttagaca tgagcaaggc tgccgaaatc ccttttcccg aaacatcggc aatacaaaac
    22201 cctacctcgt tttccgaaag cctgataaaa tcataataat caccaccaac atcaaggtgt
    22261 ggatgataaa aagaggtaat ccatatatgc tcgtttttgg gcagttcatc gtttttgggt
    22321 atgagcatgg attgcatgcg cgaagcaagt tccaactctt tttttatggc tatctgacga
    22381 aggctttcct gaaaaagcct catattttca atagctacaa tgataatgct tgaaagtgtc
    22441 tgaataaaat gaaggtgttt gatggtaggg cttacacctt cgccttcttc ttcaatgtct
    22501 ccaataagta cataagccag aggcttgcta ttgttataaa cagggatgac aatatcaaag
    22561 ctttcgctgg aagaagtgat ggatgtagaa gtgacaaaag tgatttctga atatttgagc
    22621 aggtctgcat caacgtttat attgtacgat tttgggtctt ttatccccga accaaggatg
    22681 cattcccaac ttaccccgtt atgtttgaaa ataagtattt tcccaatgtt gaggtcttcc
    22741 ttgaggattt gttcataacg tttgagcaaa tcgtctttgg gcaggttttc gttaatagcc
    22801 tgggtgatag ccaacaaggc attcagcttg aatgttgaaa tttttaacct ttctgggaca
    22861 ttggccgcca ttttcttctt tttatttcat aaaagtacaa taaaaattta atatttggcc
    22921 aaatagagta gaaacagcta atgacataac aaggattaga aaaaaatcag caattaaaaa
    22981 ttatgcttcc tcaacactaa ataacatgat attgtttttc aactcttcgg cttcatcttc
    23041 aagatgtttt gcatctgagg caagcttttc ggaagagaag ttgtattgtt ggataatgtc
    23101 atttagctca ctaattgcct ggtttatttg caatacgccg gttttttgtt caagacttga
    23161 ttcggtaatt tcctggacca aacgggctgt attttctatc tcagaaatta gtttggccat
    23221 tttatggtgc ccccgttcgg ttatttgtac acaattaatt gctctttcat ttatttcgtc
    23281 tgccactatt ttgcttcgtt cagctaatcg acgcacttct gctgcaacaa cagaaaaccc
    23341 ttttccctga tctccagccc ttgcggcctc aactgctgca tttaaagcca aaaggttcgt
    23401 ctggaaagca atatcgttaa ccatactgat ttttgcagat atatctttga tatactgtaa
    23461 gctcagttct gattcacttg ctatttcttt aacgctggct acagcagcaa aagaaatatt
    23521 ttctgtcttt ttcgaattgt ctgcattttg ttcaattctg gcaatcattt cttccattga
    23581 cgaagatacc tcttcagcag atgcagcttg tttgtttgcc ccatcggcta tttcttctga
    23641 aatatctttc atttgttcac ttgaaccagc caatttttta gcactattca aaattctttt
    23701 aataatttcg gttaaattag tgtttacttc attcagtgca tcccataaca taccaatttc
    23761 atctgatgaa atcttttgtc cattcttttt tgaaattttg ccttgtttta gtttttctaa
    23821 aacagtaagc aattctgtga ttttagaagt aatgaaccga tgaaataaat aaaggataat
    23881 gatagtagaa atgataaggc atagtattgt cccaatcaaa aaagtcagga ttatctgact
    23941 tttttgctgc tttatcttat cagccttggc gaggatgaat aaattgccaa tgcacttccc
    24001 atcctgaaaa cgcacagaag aaatgtttac ataatacagc gacccatctt tctcgatagt
    24061 tttaaaataa ggattgacaa tttcttttga gatggaatca aagccattta atatttgaac
    24121 agagtctttt ggtttatcta tatattcagg ctccgatgaa taggctacta tcccgtctgt
    24181 gccaagtatc aaacaatttt caacagtttc accaatgatg tcttcgatag tgcccttttt
    24241 ttgagatgct tcatacctaa gggagccttc agtcatcatt ttagcaggca tttccatctg
    24301 ctttttaatc ttgttctcta tttcaccaat aaattttctg ctattgtaaa aacctaagac
    24361 agataatatc aatacttctg tagctataat taaaataccg attttaaaag aaagcttgtt
    24421 cgaatatttt tgcatgtatg tttgggtaat taaatttctg acgttttacg caacatacgc
    24481 gaaaaaggtt tgataaaaaa cttttttact ttttattttc aggcaaaagt attttttata
    24541 ccttcgctac cattgataac tcatgaaaga caaaagtaat aaaccaataa aaacaatgca
    24601 aatgaagctt aaatttttaa tttttgtggc tattcttatt tcagggatta atctttgtta
    24661 ttctcagaaa tattttacca aaacaggtaa agcgtatttc atgtcccaca cagatgaaat
    24721 agatattgat gggacgagta aaagtgtggt cagttttttg gatacaaaaa ctggtgaaat
    24781 ggtttttggg gtgaacatca aaactttcga gtttacactt gcaactgccg ttgagcattt
    24841 taatgaaact tatatggaat cgcacatata tcctaaggct tcatttaaag ggaaggtgaa
    24901 agactttcaa aaaatagatt tttcgaaacc tggcaagtat gatgttgtgg ttgaaggaga
    24961 cttgacaatt cacggggtaa ccaaaaaagt atcagaaaaa ggcactttgg ttattaaaga
    25021 taaaaaaatt atagcaagct ctaattttaa ggtggccatt gctgattata acattaaagt
    25081 gcccaaaatt gtggtcaatc gtgtggccca aattattgat gtcaagattg acgctgaata
    25141 cgaaccatat acaggcaatt aatcttttcg cctgtaaaat acaatattaa agtttatttc
    25201 ttatgccagg cttgctttgt ttcaaaagtt aagcctggca ttttttttgc actaaaagtt
    25261 gtatccctaa acaaatttca tccgtgcact tgttttttta tactttttct tgcccttttg
    25321 tactttcaat aatcgcatat taaagttata tttgtgataa aatgccaacg atgaatttgg
    25381 ggcaagtaaa tatcagccaa ctagaatatg ctgcggagct gcttaaagct ttgtcacatc
    25441 cgttgcgaat agccatacta aatttgctcc tcgaagaaaa agaacttact gttacccaaa
    25501 tacactcatc gttgaatatt gagcaggctg ttgcttcgca tcatttgagc attctaaagt
    25561 ctaaagatat tttagtttca aaacgtagag ggaacaagac tttttactgc attaaaaaca
    25621 acaatttcat acaattactg gcttgtatca ataactgttc ctgtaaaaat aaataatgat
    25681 gtccgaaaaa aggttggttt taatagatag ttacgcattg atttatcggt cgtattttgc
    25741 ttttatgaaa aggccgatgt ataacaaaca agggttcaat acctctacta ttttcgggtt
    25801 tctgcttact atcgatgata tctatcgcaa acttaagcca acccatattg ctgctgcttt
    25861 tgaccttggg ggcaaaactt tccgtcatga aatgtacgaa cagtacaaag ctaaccgtca
    25921 ggcacagcct gaagaaataa gaaaatctgt cccttttatc aaagaaatcc ttcaggcaat
    25981 gaacattcat attttggagg ccgaaggtta cgaggctgat gacgttattg gcaccatggc
    26041 aaaactggct tcggccaaag actttgaaac ttttatggtt acccctgaca aagactattg
    26101 tcagctggtg tgcgacaaaa taaaaatata taaaccccaa cgttcgggca atgacgccga
    26161 aatattggat gtagaagccg taaaaactaa atattcactt gaaagccctg aacaagtcat
    26221 tgatatgctc gctcttatgg gcgacagctc cgataatatc cccggagtga aaggtgttgg
    26281 tgaagtgtcg gctaaagatt tgatagtcaa tttcaaaaat gtagaaaaca tcttggccaa
    26341 cctcgacaag gtgaagccaa aacaacgaga gctgatcgaa gccagcaaag agatgttgct
    26401 gctttcgcga aagttggtta ctatcaacac tggggctcca ataactttcg acgaagaagc
    26461 ccttttgtca aagccatttg acgatgaacg cctgttgaag ttattgcaag agctggattt
    26521 caaaacatta atcccaaagt ttgtaaaaaa tgcccagcca gcacaagctg caaagcctgt
    26581 cagtaatacc ccagatttgt ttggctttgc gatagctgat gctgaaaata ctgctgccgt
    26641 tgaaacgtac aaaactattg ccaatgttac tcatcaatac ttctggatag aagatgaaga
    26701 cagcctgaac aagctttgcc tggtgttaga gcaagctcaa atgttttgtt ttgataccga
    26761 aactaccggc ttagaccctc ataccgacga actggttggg atttctattt ctgtaaaagc
    26821 ccacgaagct tattttatca ggatagcaga tgattttgaa aaagctaaaa cttttctttc
    26881 ccctttaaaa aaggcttttg aaaatgccaa catcactaag ataggacata acctaaagtt
    26941 tgacattttg tttttgttgc ggtacaatat tactgtggca ggtccacttt tcgataccat
    27001 gattgcccat tatttgctgt atccagagca aaatcacaaa atggacacct tgtctgtcaa
    27061 gtatttaggt tacaggccta tccccattga agagttgata gggcgtaaag gcgcggcaca
    27121 aaagaaaatg aaccaggtag aaaagggaaa gctgcttgac tatgctgctg aagatgctga
    27181 tgttactttt cagctatatg gagtcctgaa agaagagatt gacaagtctt ttgcaaaaga
    27241 gcttttttac aatgttgagt caaagctcct tgaagtttta atcaatgttg aaaaggctgg
    27301 ttttacctta gatagtgcat atttaacccg gtataagaaa caactggctg aggatataaa
    27361 acaactggaa caagaaattt acaatctggc cgggcaagta tttaatatcg cttcaccaaa
    27421 acagcttggc gaaatactgt ttgaaaaact ggctatcccg tatgaaggaa aactgaccaa
    27481 aaccaagcaa tattctactg gagaagaagt gcttgtgcaa ttggaaggca aacaccctat
    27541 catagctaag attttggagt ttcgtggact gagcaagcta ctttcaacct atgtagagac
    27601 tttaccggac atgatcaaca aatataccgg gaaaatacat actaccttta atcaaacgct
    27661 tacagttacc ggaaggctaa gctcgcttaa ccctaatttg cagaatatcc ctatccgtga
    27721 agagcgaggc cgtgaagtac gcagggcttt tacctgttca gagggtaata tactcctttc
    27781 ggccgactac tcccagattg agttgcgtat aatggcacac atgagccagg atgacaatat
    27841 gatagatgct tttttgcacg atgcagatat tcacacagct acagcatcaa aggtttttaa
    27901 aatccctgta gaacaagtga caaaggaaca acgtagtaaa gcaaaaacag caaactttgg
    27961 tatcatttat ggtatatctg catttggcct tgcacagcgt atgaatatta gccgcaccga
    28021 ggccatgaac ttgatacagg agtattttaa aacctttccg ggcataaaac agtacatgac
    28081 caaaatggtg ttatttgcca aagagcatgg atatgtggac actattttgc agcgcagaag
    28141 gtatttgccg gatatacatt cgcaaaatgc cgtagtaagg ggcatggcag aacgcaatgc
    28201 cataaacacc ccaattcagg gttctgcggc agatgttatt aaaattgcca tgattaatat
    28261 ttttcataaa ttgcaggaag aaaaactgca atcgaagatg atattgcagg tgcatgacga
    28321 gttgattttt gatgttttgc cgcaggagtt agagaaagtg cggcaaatag tagtcagaga
    28381 aatggagaat gcttatccaa tggctgttcc gttaaaagtt gataccggaa cgggacaaaa
    28441 ttggcttgaa gcacattaaa aatataaaat attgccaatg agcagtaaga tattaattgc
    28501 tgatgattca aagcttgtgg tttcgctgat aaaaagtttg tttgcggaac ataatgacaa
    28561 ctatgttgtt atagaagcgg taaatggccg cgaggctatt gaaaaggcct tgagtgaaga
    28621 accagatgta atcctcatgg actggcaaat gcctgaaatg tcgggcatag atgccctgaa
    28681 aacattgaag tctgacatca ggacaaaaga aatccctgta atcatgctca ctgccagtga
    28741 agctacaaca gaagcttttg aatgtggcgc atcggacttt gtacagaaac ctttcaacaa
    28801 aaatgagctt atagcccgcg taaacactac gcttgatgct gttatggccc gaaaagagct
    28861 caaaaaacgt ttggtggaca tagaaataga aaaagaccgc ctcaaacttc aaaaagaggt
    28921 gctgatacgc cagcgcaaag agatgctaca gagcacttgg atggcagcgc aggtacaccg
    28981 ctacctgttt gccaattctg agctgctaca ccagatgtac cctgattcgt tgtacttgtc
    29041 tatgccggtt tccgaagtac ctgccacttt tttctggacc aaggtaatac agcagaacct
    29101 ttacttctgt ctcatttttc acgaaaatac aggcgttcag tctgtacttt atgcctctat
    29161 tttcaaaaaa tttattgaag aatattttca gcaaaatatc atggccaata ttgatttggt
    29221 ggaactgctc aagaaattgg agtcttcagt ggaaggattt atcaccaatg aagatatagc
    29281 tatgccttac accgagattg ttttctgtag cctcgattat tcaagcagta ccttaaacta
    29341 tgccggggta aatacctctt tgtttgcatt gaaacgcgat aagcttgttg aactcaagag
    29401 cgaacggtcg gtaaatggtt ttttcaccaa agacttagaa attgtccctc acaagattca
    29461 acttgccaag caagatattt tgtatttgct caatgacggg ttccttggct tccgaaagag
    29521 ctatcccgac agctatttgt cagatgagct aatggagttg ctaaggactg tttcgaaaga
    29581 agacatcaaa aaacaagcaa aaatgctgca aaaaactttt gagcattgga aaaccgacct
    29641 caaacagcaa acagacatgg ctgttgttgg aattcggttg taaatatttt caacttttga
    29701 ttttcagctt aataaatttg aaaaacaaat aaattctcgt acttttcaag taaatattat
    29761 cttaacctcg atgaaaaaga ttttatttat tgtgatatta ttctttatta caaagttatg
    29821 ttttggccaa gaatatgaac ccatagtgga tactgctaaa atgtgggttg tttttaacaa
    29881 tcagtgcatg aatcccccca attatggaca aacttatgct tacaaaataa aggacagtat
    29941 tttacttaat gaccaaaaat attggttcaa agtgtttgaa tcctcagatt cctcttattc
    30001 cgaatggaaa ctgaatggtt atataaggga agaaaataaa attgtcctgt attcaaacca
    30061 actttgggga atagatacgc tttataactt taacaaaacg gttggagaca ctattgaatt
    30121 caatcattgt aacattaaag tctcaggcat ttcttctgaa attattgcag gaaagaacag
    30181 aaaatcatac gatattatta atacacacaa tacttttgat gtaaaatact atgaaggtat
    30241 tggcagtaat gaaggcatac ttgctccata ttacttttgt gcgactggtg catgtagaag
    30301 ccttgtttgc ttctacaaaa acaacgaatt gttatattta aaccctgctt ttacaacttg
    30361 ttatttacat agtcaattaa acaatattgt aagtacaaag gaaatatcat tttgtattta
    30421 tcctaaccct tctacaggta taatatcaat cagtttgaac aacaacagct cttcttcaaa
    30481 ctttgatttt gttatttaca acagcttagg gacaattgta gccaaacaat ctataaatac
    30541 tgcgaaaacc cttataaacc tttcatacct ggctaaagga gtttactatt ataggcttaa
    30601 ttatcagaag aatacttata aaattggtat tttattaatt actttataac agattattta
    30661 aaccttaatc ccatgaaaac gaaaatttta attttagcct tagtaggtat ttccgcattt
    30721 ttttcatgca gcaaaagcga ttcagatgag aatagcggca atgaagaaaa taaaacaata
    30781 aaaaaacagc taaatggctt tgctcaaaag ggtccttttg taaatggctc gtcagtgatg
    30841 atttttgagc tggataactc tcttgaccaa acaggcaaag tttacaatac taccatagct
    30901 gataattcgg ggaaatttga aaaaaagaat gtcgaactct cctcaagttt tgtgcttttc
    30961 aaagcagatg ggttttattt caatgaagtg gcaaacgaaa tatctggctc acagcttact
    31021 ttgtatgcta taactgacct ttcaaaagac tcggcagtaa atgtcaatgt tttgacgcac
    31081 ctcgaaagaa gccgtatcga ataccttgtt ttgcaagaaa agaaagcttt tgctgatgct
    31141 aaaaagcagg ctcaaaaaga ggtcctggat attttcaata tttcaaaacc tgatattacc
    31201 agctcagaaa aactgagttt ggttaaaagt ggcgatgata atgctatttt gcttgctgtt
    31261 tcggttattt tgcagggaaa aaggacttct gccgaaatga gcgagctcct ggccaacatc
    31321 atcactgata taaaagcaga tggcatttta aatgacagta ctttaggcag caaattgatt
    31381 gaagatgtaa aatatatgga tttggctgct gtgcgtgcca accttataaa aaggtaccag
    31441 gaattgaacg aaagtattga ggtccctgat tttgaaaaat atgtaaaaag ctttatcaat
    31501 aacacaaagt tcaaacccaa aaaatcgatt acatatcctt atcgttcaaa atatgggttt
    31561 aacctcctca gcgatagtgt tacaaccatt gataataaaa gcaactggga ttttgcttat
    31621 tcaatgtctg caaacgttcc agatggctat agcctaaaga tagttttaaa aggcgggctt
    31681 tggtggtata gggtaatgcc aaacggtcct gttaattggg atgtatctag ttatgattca
    31741 ggcaaagagg aacaaacatt tgaagtaaaa aaaccaaata tcaatagcga tatcagtatt
    31801 caattacctt tcggggatac cattactatt gagtattatg aaaataatgc tgttgtacct
    31861 acaaaaatta aaaaggtttt ctgccctgaa acagtaacaa aataaaagca tgagggtgaa
    31921 gtaaaatgtc aaaaaaactg accgcaaagg gtacaaagtg ttagtaatcg gcctataaaa
    31981 ttttgcgtac tttatttttt ctctttgtga actttgcggt taatatttct ttttacgcag
    32041 cctcagtttg actcaaacct tttattaatc agccagtgga ctctaagaag ggtttaagcc
    32101 aaatttttat aacaattact gttaatgtca atatgcatac acctcaaagc gtatagcata
    32161 ttggttgtat tgattcagcg cttctatata gtcaaaataa tactgttcgc gtttgaccag
    32221 caggtttagg taagtaatat ctgcttttcc gctacgtgca atatattgtc gggcggcttc
    32281 attgtatttt ctggcttgct ggcggccaaa atcgtagtaa aagttgatac ggtcaaaaca
    32341 ttggttcatg cgggcaagca attcatctat ctctgtctgc atttccagca tggttttctc
    32401 tgaatgcagt gtgatgcgtc tttgttgtgc ttttgcatct tttgtttttc ctgaaaaaat
    32461 aggaaccctt attcctgctt cccaggcatg taagccgttt gagccagata catcaaggta
    32521 tttatagcct acaaaaaatg aaggtaaagc ttcattttta accatcctgg ttttgatgtc
    32581 gtt
//
LOCUS       DAOSPF010000038        31641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1452_length_31641_cov_7.497151, whole genome shotgun sequence.
ACCESSION   DAOSPF010000038 DAOSPF010000000
VERSION     DAOSPF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_31641_cov_7.497151"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..720
                     /locus_tag="PK252_08740"
     CDS             28..720
                     /locus_tag="PK252_08740"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT14833.1"
                     /translation="MFTQSFLYYPILFTDFINFSRIAENITPEQLVSNLDQYFIKFDS
                     IINKYNLEKIKTIGDAYMCAGGIPAANKSNPFTVVLAGLEIQKYMKALRVKTEDTDNP
                     SWKLRLGIHSGELVTGVVGEKKFAYDIWGDSVNTASRIESAGEAEKVNISGSTFNIIK
                     DYFDCSYRGKIKVKNKGEIDMYFVNRIIPEFSADKEGLEPNEKFMNYLKSLDLPVLDA
                     ESYKDSNSIFRA"
     gene            complement(1072..5232)
                     /locus_tag="PK252_08745"
     CDS             complement(1072..5232)
                     /locus_tag="PK252_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT14834.1"
                     /translation="MYELFTFVISDMILKTCNIIKFLLVSTLLYIQALGYSQKQLFFH
                     LGVEDGLPNGVINCIAQDKTGFIWIGTNDGLIRYDGKYFKNFSYIPGDSTSLINNTIQ
                     DILCEGYNVWICTNKGVDRYNSKTERFSHIPILDDGLKQSYAAFKIFKDLKGHLLIAT
                     QMGLYIYDEFEHAIKPSEVEFGRKPIEISSISQDRDNDYWIGSRNYGVFTFNSKSKVA
                     KPVVYMLNGKNALQNNKIFCLYEDNQHTVWIGTEEGLFSINKDTWQMERYVPVAGRND
                     ALPHISVNKIVEDSRNNLWIGTNGGLAKFNRKDKRFINYYHDDNDPNSLTDNSIHSIF
                     EDAQNNLWIGSGESGLNISRLHDIEFNNFVRKPNDPLSLSYAFVSSVLEDSQGNIWVG
                     TNGKGINKYDAKTMRFQYFIPPSSNQVGTQTAAILSLIEDRNKNIWIGSYLGGLTVYN
                     PKTQQYKTFEVNPADSGALNNNIVNCILEDSKGNIWVATNGGGVNIYQPATQKFKHIR
                     TGNSALSSDYCLVVKEDAQSNIWVGSYYGLNKIDTKTLKVTRFVNSSSPGAISADVIY
                     SIYTDAKGRIWVGTNYGLNLYNPVNNTFTVFTTQNGLPNNVINGILGDNRGFIWLSTN
                     RGLCKFDPENFRVVNYDKQDGIASLEFFHGACFKSKKGMLYFGGIRGLTFFNPDIIKT
                     SSARMPLVLTELLVFNQVVKPGKNSILDKSITYTDEVKLKYNQSFITIGFAALNFSTS
                     SKDNYSYYLDGIDRQWNNVGNNRYASYTNLPAGNYTFYVKSFNNAGLENQVSLKIIVK
                     PAFYKTTFAYILYILLFIGLSYFTYSYIHSRTVYKHNLLIERMEKEKAVEINQAKIKF
                     FINVAHEFKTPLTLIVSPVEKLLSSAKSLSFSEINHLHLVIYRNSMRLSRLMNQIMDL
                     RRIDTGNIKLYVTEKEIVSFVREITLSFEDYAEKHNIKFSFESNVEHLNVWFDADKLE
                     KVIYNLISNAFRYTPDGHSVKIIVEHAQPEGNTEGIVRIIVEDKGIGIKKEHQKEIFN
                     RFFQVPSEYSANPASSGIGLSIANEFIEMHHGHIAVDSEPGQGSTFTVTLPLGNKHFD
                     KEEIIIANESSELYNPEFVVSETVEDEEANESENSLPAAGSASKRKFKLMIAEDNYEL
                     RNFLATSLSDTYTIFEAANGKEGLDLVNDHMPDIVITDVMMPVMNGIELCKAIKTNIK
                     TSHIPVVILTVLNNYDNQLEGLETGADDYITKPFNISLLRARLINLIENRKKLIKRFV
                     DEMKPEPDKLAQNSLDSKFLTKAFEIVEKNLTNTEFSAEDFASQIGMSRSNLHIKLKA
                     LTNQSATEFIRLYRLKKAAELLSNSQYNISEVSFMVGFNSISYFNRCFKLQFGLTPTE
                     FMEKGPTS"
     gene            complement(5332..6459)
                     /locus_tag="PK252_08750"
     CDS             complement(5332..6459)
                     /locus_tag="PK252_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT14835.1"
                     /translation="MSTSFYQIIFASFILCLTGCRQSSSPADSDITVDANATYETKAL
                     FHNLRANTSKGILFGQQDATAYGVGWKDEDLKSDVNEVCGSFPAVYGWDVSGIGGEYN
                     IDSVRFDRMKFWIKSAFERGGINTVSWHLQNPVTDGSCWDTTTAVRHILPGGKFHNKY
                     NRQLDLLADFFSQLKSKNGKLIPVIFRPFHEANGHWFWWGARSCTDDKYIQLFRYTVS
                     YLRDVKGVHNLLYAYSPDIFKTKENYLKRFPGEEYVDVLGVDNYYNFRSEDIVSEGIA
                     QLRIVVELAKSMNKISALTETGLDAQTDSTWWTKNLLYPIINDSVARNIAWILVWRNA
                     DAKHFFVPYPGQTAVQDFIKFENHPFTVFERDLPKMYQFEK"
     gene            complement(6562..7761)
                     /locus_tag="PK252_08755"
     CDS             complement(6562..7761)
                     /locus_tag="PK252_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOT14836.1"
                     /translation="MLNDRTNILKEAAFHEVTKNILPYWMNNMVDNENGGFYGGIDFY
                     GKLDPKASKGGVLNARILWTFSRAYNVLGDTKYLEMAKRAYQYLEKYFRDPEYNGIYW
                     ELDYTGKPINTQKYCYVQGFYIYGLTEYYRAANDASALEQADKVFHLLEKYARDKKQN
                     GYFEAFARDWTVTDNVRISAKDLNENKTHNTHLHILEAYTNLYNVCKKPEYKEALDNL
                     IVLLAEKFYNPENGHFKLFFDDDWNAKGELVSYGHDIETSWLLWEAILASGSDEMKEK
                     YEPIVLHIVDVASRESFDADGGQYYEGNPRGVVDTDKHWWPQAEAVVGFINAYDITVN
                     EKYLDYAAKSWQFIEDYFVDKERGEWHNKLTKDRKPIESEMRAGFWKCPYHNSRVCFE
                     LITRLSK"
     gene            complement(7845..9026)
                     /locus_tag="PK252_08760"
     CDS             complement(7845..9026)
                     /locus_tag="PK252_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOT14837.1"
                     /translation="MTNVQFDEKVKQLFAEHDKFIMRKNVACEDKNGIYVRYQYPILT
                     GEHAPVFWRYDLDYKTNPLLLQRIGVSCAFNSGAILLDGRYLLVVRVEGWDRKSFFAV
                     AESPNGIDQWRFWDYPVDLPDTDKEETNVYDMRLVKHEDGWIYGIFCSERKDPNAPAG
                     DLSSAVAKAGIVRTKDLKKWERLPDLKTKSQQRNVVLHPEFINGKYALYTRPQDGFID
                     AGSGGGIGWALIDSMENAVVKEETIIEPRVYHTIKEVKNGEGPAPIKTSKGWLHLAHG
                     VRGCAAGLRYVLYMYMTDLKEPWKVIATPGGHFMAPEGEERVGDVSNVLFSNGWISNE
                     KGEVYIYYASSDTRMHVAVSTIDKLVDYCLNTPADGYRTHSSVATLKKLIEKNLSIIK
                     K"
     gene            9498..10007
                     /locus_tag="PK252_08765"
     CDS             9498..10007
                     /locus_tag="PK252_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14838.1"
                     /translation="MFDKFENGDFIAIEAIKDLTFEEYKYVYVKVINDCKKKVKTTQS
                     GAFIVRPSKAIAMRYIKELLRTFDYDYIKTEEIITYSNSFKFSIFPNPVSSTSIICFN
                     MQNTAKVSAEILDLEGKTISICIKEQMLNAGKHEYPLGLPSKIKGNFLVRLWVNNVVN
                     VQLITKYAN"
     gene            10035..10955
                     /locus_tag="PK252_08770"
     CDS             10035..10955
                     /locus_tag="PK252_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14839.1"
                     /translation="MKKVLSILSNSYLMNEPELVNKTKYILLYISNNLLIRLYCVCMC
                     FLYSCEPENNSVFKIHFSDGNRITDSDIIFYDSSSHCLFLKNEIKIKAKTDFSVIVNK
                     QTIYRGVVQSKLDSNSPFSPFYINEVNSIFGKNIIEIQYNKNYPPDLRNDPQIINSLK
                     SRNFLRNGISCNIDSIVIENAAIGNVNVSYNLTIINNDNYNYYVINKGFVDVMFMNID
                     TYALWYLKSNPTQEEMPVYTLNNFSLLKSNSKLTRTVKFLNSYRMEPGYYQVTCTILH
                     GGFENLSINQDSGRIWLGKAISVIDSLRID"
     gene            11112..12155
                     /gene="thiL"
                     /locus_tag="PK252_08775"
     CDS             11112..12155
                     /gene="thiL"
                     /locus_tag="PK252_08775"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT14840.1"
                     /translation="MEKKIDQKFTPLKELGEFGLINHLTKDVIIHQPNTVKGIGDDAA
                     VLDTGTVYQVITTDLLAEGIHFNLVYTPLKHLGYKAVAVNLSDICAMNAKPKQITVAL
                     AISSKFSVEHLNELYSGIKLACQKYGVDLIGGDTTSSLTGLTICITAIGEVEKDKVTY
                     RNGAKNTNLLCVSGNVGAAYLGLQILERERKLFEKEGITQPDFGNYTYLIERQLKPEP
                     RTDIVKWLEEKNIVPTAMIDISDGLSSEILHICKSSNTGCRIYQEKIPLHEQTADFAE
                     EVNMHPINAALNGGEDYELLFTVDIKHYEALLQNDQIRIIGHITEQVEGTNLVLENNE
                     FVNLASGGWNALN"
     gene            complement(12254..13717)
                     /locus_tag="PK252_08780"
     CDS             complement(12254..13717)
                     /locus_tag="PK252_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14841.1"
                     /translation="MSPILVFLLILLGSFPVATVVIHLLFKKTVVAKIARVVYVVSAG
                     VAFLAFVNGQLGLKALIWVLPVMIIWLLSSNIMLIKIIQKPIIGLKENIDELTNGNIK
                     IEVDKNIAAQENEIGDIAKSIEKLTAQLQQIAENILKSSDSLVNLSDKIKQGADQLSS
                     GASDQAASAQEVSSSMEEMVANIQQNTDNSKQTEKIATESANGIKKGNESVITAADSM
                     KKIAEKISVIGDIAFQTNILALNAAVEAARAGEHGRGFAVVAAEVRKLAEHSKVAADQ
                     INELSRQGVTISETAAMNLNSIAPEIEKTAKLVQDITAASIEQNAGAEQINNALQRLN
                     HVIQQNAVSSDSLAQSAIELTKESENLKEITTFFRLDSKKKAKVEEELKKEIKKEEPK
                     IIKKIDKKLDEIASARHERLEKLKSDIRSSVIGIEEEKPIAEKDKAIENTPVAEKPAA
                     LKVTSKKEPTKPETKNSGFNLKMFDDDAGKDSEYERF"
     gene            13948..14517
                     /gene="nrfH"
                     /locus_tag="PK252_08785"
     CDS             13948..14517
                     /gene="nrfH"
                     /locus_tag="PK252_08785"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOT14842.1"
                     /translation="MKKWFFKIIPSEEWHFTAIVTLGIVFGLGFYIFYISKAWSYLSD
                     RPETCVNCHVMATEYVTWFHSSHKRGVCNDCHVPHQNFLVRYLFKAKDGLRHATIFTL
                     RLEPQTIKMNEAGVEVVGENCLRCHKAVFGRLFQNAKQTQEIKSRDCWNCHRETPHGR
                     VKSLSSVPNARVPLPESPVPEWLSHLLKN"
     gene            14533..15990
                     /gene="nrfA"
                     /locus_tag="PK252_08790"
     CDS             14533..15990
                     /gene="nrfA"
                     /locus_tag="PK252_08790"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HOT14843.1"
                     /translation="MKAVNQIIKEKPWMGWVIFFITMVVTFFIGLLASSIMERRTEAI
                     IINKPKADIKAFESDNKVWGQYYKDEYQSYLQTADTSFKSKYGGSATIDMLEEAPEMV
                     VLWAGYPFSKDYRQGRGHYYAVDDIITTLRTAAPAKPGTDPMPATCWTCKSPDVPRLM
                     NKMGIDSFYHQKWSTFITEIKNPIGCADCHDPATMDLQITRPALLEAFKRNGKDIAKV
                     SQQEMRSLVCAQCHVEYYFKGDGKYLTFPWDKGMDVDNIEKYYDAIEFSDWTHSISKA
                     NMIKAQHPDYEIYSMGIHAQRGLACADCHMPYKSEGGRKFTDHHIQSPLNNISNTCQI
                     CHRESEAELLRTVYERQDKIAEQKQILEKLLVKAHYEAKAAWDNGATATDMKPALTEI
                     RHAQWRWDFAAASHGAAFHAPIEVMRIIATGIEKTQQARLILSKISAKKGIIEDIAIP
                     DISTKANAQKAVGLDMPTIEKEKQLFLKKLKTDWK"
     gene            16075..16338
                     /locus_tag="PK252_08795"
     CDS             16075..16338
                     /locus_tag="PK252_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14844.1"
                     /translation="MRFVRLRFQLQTPLIDYSLIKYLWLWQSVSRTEALTATEANKNN
                     NGIDSTDELREYVSVQEAKASGSIQHPAVDRDNIYQKFGFPLK"
     gene            complement(16413..17453)
                     /locus_tag="PK252_08800"
     CDS             complement(16413..17453)
                     /locus_tag="PK252_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOT14845.1"
                     /translation="MDAIYNSIEKSSKELTKEDFQSGQMVKWCPGCGAHVILASILNT
                     FPKIGYNQDKFVFVSGIGCSSRFPYYINTYGFHGIHGRASAIATGVKLANPGLSVWVA
                     TGDGDSLAIGGNHFMHAVRRNIDLNIILFNNQIYGLTKGQFSPTSPMGSVTKTSPFGT
                     IEPPVNPGKLVLGVGGSFFARVPDNNAHLLGEAVYAAAKHDGTSVVEVLQNCIIFNDK
                     AHEAWVGKEAREENMLVLEHGKPMLFGKNMKKGLMFKNGKLLVVEVGKDGVKESDILV
                     HDMYDDNPGLQIMLIEMAPPHFPVAVGIIRAAKRDTYDDLFYEQIESAKKSSDIKCVD
                     DLLNSGTVFEIR"
     gene            complement(17475..19313)
                     /locus_tag="PK252_08805"
     CDS             complement(17475..19313)
                     /locus_tag="PK252_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT14846.1"
                     /translation="MTKKTKVKELDNVVVKFVGDSGDGMQLTGTLFSDSAAVFGNDLA
                     TFPDYPAEIRAPHNTVAGVSGFQVHIGKSKVNSFGDLCHVLVAMNPASLKANLKVAER
                     GATIIVDVDAFDEEAIKKAGYLSNPLEDKSLEGYNLIKAPITTLTRGALADSGLNKKQ
                     VDKTKNMFALGMIYYLFNRDLQHTIEYLSDKFSEKGEMLEANKKVLQAGYDFADTIEA
                     LDSTFQVSPAKLEKGKYRNITGNVAAAWGLLAAAEKANLPLYLGSYPITPATDILVEL
                     SKHKSLGAKVFQAEDEIAGITSAIGAAFAGNLAVTTTSGPGLSLKVEAMGLAVITELP
                     LVIIDVQRAGPSTGMPTKSEQSDLYQALFSRNGDAPIPVIAASSSANCFYYAFEAARI
                     ALEHMTPVILLTDGFLGNGSQLFRIPKMADLPVINPPIAAPNEPDYKPYRRNPDTLVR
                     RWAFPGTPGLRHRIGGLEKENVTGVVTTDAANHELMVKLRAEKVNKISSRIPKQTVTG
                     AQQGDLLIVSWGSSEGVIQTVVESMAADGKKVGHAHFNYIMPLPTNVDEIFNNFKKII
                     VAEQNMGQFVNYLRMAHPAFQYEQYNKVQGLPFTETEIKNVISKLL"
     gene            19870..20667
                     /locus_tag="PK252_08810"
     CDS             19870..20667
                     /locus_tag="PK252_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT14847.1"
                     /translation="MDSNNPMPNFRELEQNSLEKAFLKNLNHELRTPMNAIIGFANLI
                     QNHCHGDKEIAELVEAMNFSCFQLLNLVNDLVDLSKLETNKLHLHPVKCDLNELMESL
                     YIDYQKQLDKDNKPIELLIYKDDCYRDIFIDIDRARFTQIWKYIIDNAMKFTHIGKIE
                     FGYMIRGRGVHCYVKDTGIGMPQSSVKMVFEQFVRNSNCEYGGLGIGLTIARKLIELM
                     EGHIWIDSEQGKGTIVYFTVPVEPENKKKKKDELIDKLIIIDQKQLQ"
     gene            20809..22662
                     /locus_tag="PK252_08815"
     CDS             20809..22662
                     /locus_tag="PK252_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT14848.1"
                     /translation="MKKTLLVSILAIASVIGANAQSINDPFFEKVSYVGAFGNEDWTK
                     GWANFDPQNTDYPETTTTLGNGELSYAAGYKITSNTSISGVVKLSGWVYVKDGATLTI
                     EKGTIIRGESSAALIVERGGKLIAEGTVDKPIVFTSIKPKGSRAASDWAGVIICGKAT
                     NNKSNNTLIEGGVGAYFGAGDQAVENDNSGILKYVRIEFPGYDIDGAGNEINGLTMGS
                     VGSGTTIDYVQVSFSGDDAFEWFGGSVNCKHLIALATEDDDFDTDNGYVGKVQFALAV
                     RDPKVCDTDGARGFESDNDANSSYNKPYTAAVFSNVSLFGPKTDESANQKHDVAMLLR
                     RNTRLQIYNLFSTGFVKGGVVIDGDVTQKAAGEDSLVIKNSIFAGYSKFISTKGTNNP
                     LSNAKAWLVNGKNDTLNNLDNVKVQFPIVVTAPVFIPESGSVLLTKGSSFGAAVTSIV
                     ISAAGNVTSIKTTETLQLSATVTPDNAVSKEVSWSIESGSDVASVSESGLVTPLKAGN
                     VTIKAVAKDGSGISSTFALEVKSNVAVGSTSVATASIWPNPAMDLVQVKCENEISSLE
                     VLNNVGQIVLKVANINSNNSSANISNLNSGIYFMKLTMSNGAVSVNKIVKK"
     gene            22955..24127
                     /locus_tag="PK252_08820"
     CDS             22955..24127
                     /locus_tag="PK252_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT14849.1"
                     /translation="MAENTNIQLELRKFVAPEFIFGLDARMLAGRYVKKLGAKKVLVV
                     TDAGIHTAGWANDVLETLDDEKLSYQIFSEVSPNPRSAEVMKGAEVYKNSNCDAIVAI
                     GGGSVMDCAKGIGIVSSNDKNILQYEGVDKVDIPIPPLVCIPTTAGTSADVSQFAIIN
                     NEHDKVKIAIISKAVVPDVALIDPAVLVTMDSYLTACTGMDALVHAIEAFVSTASSPF
                     TDLNALEAIRLINENLVSSINLPNDYELRGKVMLGSLYAGLAFSNASLGCVHAMAHSL
                     GGFLDLPHGECNAILLPHVMSYNFESATAKFAKIGEVLGLEMKGLLMNEKRKMLIEHI
                     MQLKTKTGILKTLRERGVGSSDIPLLASKAIKDPCQITNPRTPSKEDIEVIYSEAM"
     gene            24099..25847
                     /locus_tag="PK252_08825"
     CDS             24099..25847
                     /locus_tag="PK252_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14850.1"
                     /translation="MKLFIVKLCNMGDLQDRDSLRDKIIGLGENSARKSYYPELQKKI
                     KELEEREQSLYDLINNIFDAIIIHDEEGTVLAVNEGMLAMYRMPDKETALQYTIMDYS
                     FGYEPDVVKEVFKKTLGGKPQIFEWRARRPLDNSSFESEVSARTATWQGKKVIIATVR
                     DITERLQTQLKLIEREQQLECQNEELISLNEELAEQNVRIEQFNEQLKIANNKAVESD
                     RLKTAFLANMSHEIRTPLNGILGFTSLLPEARNSDEAFEKYISIIQTCSNQLLTLIDD
                     IIDLSKIEAGMMNFNPQLLNINNFMQQLCAYYKSTPSKAELICDIESRNDELTIFVDQ
                     ERLRQVFNNLLNNAIKFTSSGKITFGYHIKNQEVEFYVSDTGIGIAPENTEIIFEHFR
                     QVDVGFTKKYRGTGLGLAICKALLELMGGRIWVESVLDKGSTFYFTVPLSFEKEDANV
                     PEANKAFSVEALKGKTILVAEDEEFNYMFINELLSKADAKLIHVLDGAKAVETVKQNP
                     SIDLILMDIKMPVMNGIDATRIIKKTYPQIPVIAVTAYAMADDKEKALEAGCDGYLAK
                     PINKAALFAAINQCIK"
     gene            26099..26476
                     /locus_tag="PK252_08830"
     CDS             26099..26476
                     /locus_tag="PK252_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOT14851.1"
                     /translation="MKEITKAQEDILQALWQINDGAVSDVLDILPEPKPAYNTVATVI
                     KVLEKKGYVSHKTYGKTNVYYPLVSKQDYARNVLKNSVKGLFNGSFKQAVSFFVRENK
                     EVSLAELEELRNYIEEEIKNKKN"
     gene            26491..28002
                     /locus_tag="PK252_08835"
     CDS             26491..28002
                     /locus_tag="PK252_08835"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HOT14852.1"
                     /translation="MYYLAGSSLCLTLFYCVYYFLFSKESGHRFVRFYLLFSVLVSLL
                     LPLSSYSIRLNNVYIETPKTESAIKSDIKNDNVAISPIKEEVSAVYHSPDKPVNYWVI
                     VVWAYWLISAVVFLYFIIGIINIYWHYRQSKKEQIGNVVILRNESLSNSFSFMNLIFL
                     NPNGLSEKETDSIVAHENIHVSQYHTIDLLLIELVAAVMWFNPFVWLFRKSFRQVHEY
                     LADEGVLNIGFDRLEYQALLINQVAQGRLLSLACNFNQSLIKKRIMMMTKIKSGNKAK
                     LKLLAIVPLTLGLFLMIACANGHNINKVTAVETVRMNVLYLGVDNPIKIAASGYDPSE
                     LDVTIDNGKIFGKNGQYIVRPEKDGQAWLTVSCKGKVVQLTSFRVKIVPDPAAMIPVQ
                     EQSGYLFRMLKSGEISKKELLSATGLMAGMENFDFDIEFKVASFVLSVTVPNSMEVKE
                     EISNSEKFSKGQIDLIKSLVVNQKLTVENITVVGSDGTKRKLSPLVFTIVADK"
     gene            complement(28043..28687)
                     /locus_tag="PK252_08840"
     CDS             complement(28043..28687)
                     /locus_tag="PK252_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOT14853.1"
                     /translation="MKFIKLFATVVIFTAISCTNNQQPSASIDNLPAPQIKGGKPLML
                     ALRDRKTTRDFSDKELSLQQISDLLWAANGINRPAEKRHTAPTARNRQELDIYVAMKS
                     GVYLFDADNQKLIKQSDKDIRAEIGDQDFHKIVPLTLILVADLNKAFEGDTTRNDYET
                     IDAGYISQNIYLYCASENLATVAVGWINRPKIDSLLHLSSRQRVIIAHPVGYPK"
     gene            complement(28747..29769)
                     /locus_tag="PK252_08845"
     CDS             complement(28747..29769)
                     /locus_tag="PK252_08845"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT14854.1"
                     /translation="MIVVLGGTGLVGAHLIYELAVNGNMVKAMYRNKESIDNAYRIFS
                     FYYDDIEKLSRHIEWVQGDIENLTILEEILEGASTVYHCAAQVSFSSSGKRRMLENNI
                     NGTANVVNACLNKGVKRLCYMSSVAAIGHAGNGELINEEMIWTPSKERSTYSVSKFHA
                     EMEVWRGIEEGLSAVIVNPSIIVGPGIWGKSSTAMFDAVYKGMRFYTPGASGFVDVRD
                     VVNVMIMLTNSSIKGERFILNSENLSYKDFFSLVAHSLGKKAPSIEVSRWMAEFGWRA
                     IWILSKITGKAPQITRETVDSGFSSHAYSNKKITDTLRFKFTPIAQSVKDTAKVYLEE
                     KRLIKE"
     gene            complement(30304..30750)
                     /locus_tag="PK252_08850"
     CDS             complement(30304..30750)
                     /locus_tag="PK252_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT14855.1"
                     /translation="MSHSEGQNIVLPDKKIVSFIKKHHVLTLATCQNNIPWSCSCFYA
                     VSEDPLQLIFSSSPETRHVKEFEKNEIVSVAIALETKIIGKLQGVQMSGKIIVDTQGL
                     QLSKAYLKRFPYASVMDLNLWAFEPEFIKFTDNTLGFGKKLTWQKG"
     gene            complement(30764..31414)
                     /locus_tag="PK252_08855"
     CDS             complement(30764..31414)
                     /locus_tag="PK252_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT14856.1"
                     /translation="MAKNFQATEGYTYIKKLISEGEHTTLDFKFEISNSRKIARTLVA
                     FANTQGGRLLIGVKDNGAIAGVRSEEEFYMIEAAASMYCKPEIHIVVRPWLIEQKTVL
                     EVIVEKNPKLCYAKDENNKWIVIVRINDQNIVANRVYVEAQKIKRNKNIKLSKVTETE
                     QLILANISDYKHVSISKLSRLLHLTIRQTEDALINLIAREMIAINLNDKEITYSGK"
     gene            complement(31431..>31641)
                     /locus_tag="PK252_08860"
     CDS             complement(31431..>31641)
                     /locus_tag="PK252_08860"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOT14857.1"
                     /translation="LVISDNIVFLKDKNKWVGTIYDPESGNTYNCKLHFEEDKDILKL
                     KGSLKGVRALAHETICIREKRLRTD"
BASE COUNT         9828 a         6024 c         6047 g         9742 t
ORIGIN      
        1 ggcagcatta tctggttact tttatgagtg tttacacaat cttttttata ctacccaatt
       61 ctttttactg attttatcaa tttttcgcga attgctgaaa atattactcc tgaacaatta
      121 gtttctaatc ttgaccaata ttttattaag tttgattcaa taataaataa atataacctt
      181 gaaaagatta agacaatagg agatgcatat atgtgtgcag gtggaattcc tgctgccaat
      241 aaaagtaacc cttttactgt tgttttagcc ggccttgaga tacaaaagta tatgaaagct
      301 cttcgggtta aaacagaaga taccgataat ccttcgtgga aattgaggct tgggattcat
      361 agtggcgaat tggttacagg tgttgttgga gagaagaaat ttgcatacga tatctgggga
      421 gattcggtta atacggcaag tcgcattgag tctgccggag aagctgaaaa ggtaaatatt
      481 tcgggttcaa catttaacat tattaaagat tattttgatt gtagttatcg ggggaaaata
      541 aaggtaaaaa ataaagggga gatagatatg tattttgtaa accgtattat tcctgagttt
      601 tctgctgata aagaagggct tgagcctaat gaaaaattta tgaattacct taaaagcctt
      661 gatttaccag ttttagatgc agaaagctac aaagattcca atagtatatt tagggcttag
      721 tcatttagtg tatggcctat gctaattatt gtatttttgt gttttcattg atacatataa
      781 aaaagatggt aatttgtata gtcttgaaag aagttgaatg tatgttgcct gttgttgtca
      841 ggtttgtgtt ttataagtta taccagatta atcattagga ggctttttaa agccgaacta
      901 tatgatttag ttgggtaatc atctcgttat aaacaggaca acctgattta aaaattgttt
      961 gaaaaatttt attaagttat tgaatttcaa tgcgatatat taaatttttc agtttataca
     1021 caaatatgtg cattttatcg agactggtca aggctttgtt tggctgaatt ctcaggatgt
     1081 tggtcctttt tccataaatt cggtaggggt aagcccaaat tgaagtttaa aacagcggtt
     1141 gaaatacgag atgctattga accccaccat aaacgatact tctgagatat tgtactggct
     1201 atttgataaa agctcagcgg ctttcttcaa cctgtaaagc ctgataaatt ctgtagctga
     1261 ttggtttgtc aacgctttta gttttatatg caggttactg cggctcattc caatttggct
     1321 ggcaaaatct tcggcagaaa actcagtatt ggtaaggttt ttctctacaa tctcaaatgc
     1381 tttggttaag aacttactat caagcgaatt ctgagcaagc ttgtcaggct caggtttcat
     1441 ctcgtccaca aaacgtttga tcagtttttt cctgttttca ataagattta tgagccttgc
     1501 gcgtaaaagg ctaatattaa agggctttgt aatataatca tcagctccgg tttctaatcc
     1561 ttcaagctga ttatcgtaat tatttaacac agttaggata acaacaggga tatggcttgt
     1621 tttgatattg gttttaatgg ccttgcatag ctctatccca ttcattacgg gcatcatcac
     1681 gtctgtgata acaatatcag gcatgtggtc gttcactaaa tccaaacctt ctttgccatt
     1741 ggctgcttca aaaatagtat atgtatcact taagctggtg gccagaaaat tgcgaagctc
     1801 ataattatct tcagcaatca taagcttaaa cttacgtttt gaagctgaac cagcagcagg
     1861 caaagaattc tcagattcat tagcttcttc atcctcaaca gtttcagaaa ctacaaactc
     1921 agggttataa agttccgaag attcattggc gatgataatt tcttctttat caaaatgctt
     1981 attgcctaaa gggagtgtca cagtaaaagt acttccctgc cctggctcac tgtcaactgc
     2041 aatatgtcca tggtgcattt caataaattc attggcgata gaaagaccaa tccctgagct
     2101 tgcaggattt gcagaatatt cagaaggtac ctgaaagaac cggttgaaaa tctccttttg
     2161 atgttctttc ttgataccaa tgcctttatc ttcgacaata atcctgacga taccttctgt
     2221 attgccttca ggttgggcat gttcaactat tattttaaca gaatgtccat cgggtgtgta
     2281 tctgaaagca tttgaaatta ggttataaat gactttttca agtttatcag catcgaacca
     2341 aacattaaga tgctcaacat tagattcaaa tgagaatttg atattatgct tctccgcata
     2401 atcctcaaag ctcaaggtta tttcgcgtac aaagctcact atttccttct ctgtaacata
     2461 aagtttaata ttaccagtat caatcctgcg taaatccatg atttggttca tcaaacgaga
     2521 caaacgcatt gaattacggt atattaccaa atgcaggtga tttatttcac taaaagaaag
     2581 gctttttgca gacgacaaca gcttttctac cggagataca atcaatgtca gaggcgtctt
     2641 aaactcatgg gccacattga taaaaaactt gattttagcc tggttgattt caacagcctt
     2701 ttctttctcc atcctttcta tcagcaaatt atgcttgtaa acggtacgag aatggatgta
     2761 gctgtacgta aagtatgaca acccgataaa aagcaaaata tacaatatgt aagcaaaagt
     2821 agttttataa aatgcaggtt ttacaataat tttaaggcta acctgattct caagccctgc
     2881 attattaaag gatttgacat agaaagtata attacccgct ggcaaattgg tataactggc
     2941 atatcggtta ttgccaacat tgttccactg gcggtcgatg ccatcaagat aatatgaata
     3001 attgtcttta ctcgaggtgc tgaaattaag cgctgcaaac ccaatggtta taaaggattg
     3061 attgtatttc agttttacct catctgtata agtaatagat ttatcaagga tagaattttt
     3121 accaggcttt accacctggt tgaaaaccaa caattctgtc agaaccaatg gcattctggc
     3181 agaggatgtt tttataatat cagggttgaa gaatgtaagg ccgcggatac ctccaaaata
     3241 caacatccct tttttgcttt taaaacatgc tccatgaaaa aactcgagag aagcaatacc
     3301 atcttgctta tcataattca ccacccggaa attttcgggg tcaaacttac atagtcctct
     3361 attggtgcta agccagatga agccccggtt gtctcccaaa atcccattaa tcacattgtt
     3421 gggcaggcca ttttgagttg taaatactgt gaatgtatta ttaacagggt tatagaggtt
     3481 caatccatag ttagtgccaa cccaaatcct gcctttagca tctgtatata tagaatatat
     3541 tacatctgcg cttatagcgc ctggagaaga gctattgaca aagcgggtta ctttgagcgt
     3601 tttagtatca attttgttga gcccgtaata agagccaacc caaatgttgc tttgagcatc
     3661 ttctttaacc accaggcaat aatcactgga caaagctgaa ttgcctgtgc ggatatgctt
     3721 aaacttttgg gttgcaggct gatatatatt tactccgcca ccattggtag ctacccaaat
     3781 attacctttt gagtcttcca aaatacaatt aacaatattg ttgtttagcg caccagagtc
     3841 ggcagggttc acctcaaaag tcttatattg ttgggtttta ggattatata ctgtcaaacc
     3901 tccaagatac gagcctatcc agatgttttt gtttctgtct tcaattaaag aaaggatggc
     3961 agcagtttgc gtgcccacct ggtttgatga aggaggtata aaatactgaa agcgcattgt
     4021 ttttgcatca tatttattga tgcctttccc attggtacca acccaaatat ttccctgact
     4081 gtcttcaagc acagaagaaa caaaagcata gctaaggctt agcgggtcat tgggttttct
     4141 gacaaaattg ttaaactcaa tatcatgtaa cctgctgata ttcaatccgc tctcgccgct
     4201 tcctatccac aggttgtttt gggcatcttc aaatattgag tggatagaat tgtcggtaag
     4261 tgagttgggg tcgttatcat cgtgataata attgataaag cgcttgtctt ttctgttgaa
     4321 ttttgctaaa cctccatttg tcccaatcca taagttattg cgggagtctt ccactatttt
     4381 gttgaccgaa atatggggca aggcatcatt tctgccggca acaggcacat atcgttccat
     4441 ttgccaggta tccttattaa ttgaaaataa tccttcttca gtcccaatcc agaccgtatg
     4501 ttgattatct tcatacaagc aaaatatttt gttgttttgt aatgcgtttt tcccattcag
     4561 catgtaaact actggtttcg ccactttact tttcgaattg aaagtgaaca ctccgtaatt
     4621 tctgctacct atccagtaat cattatctct gtcttgcgaa atggaggaaa tttcgatagg
     4681 ctttcttcca aactcaactt ctgagggttt aatagcatgt tcaaactcat cataaatata
     4741 cagccccatt tgagttgcaa tcaataagtg ccctttaagg tctttgaaaa ttttaaaagc
     4801 agcataactc tgcttcagcc cgtcgtctaa aattgggata tgagaaaacc tttcggtctt
     4861 tgagttatat ctgtccactc ctttgtttgt gcaaatccaa acattatagc cttcacataa
     4921 aatatcctga atggtgttat ttatcaaaga tgtagaatct ccggggatat aactgaaatt
     4981 tttgaaatat ttgccatcat agcggataag cccgtcgtta gtgccaatcc aaataaagcc
     5041 tgttttgtcc tgggcaatgc aattaataac cccgttggga aggccatctt caacgcccaa
     5101 atgaaaaaac aattgttttt gagaataccc caaagcctgt atatacaata acgtagatac
     5161 aagtagaaac ttaataatat tgcaggtttt caatatcata tccgagataa caaaggtaaa
     5221 caattcgtac ataaactacc atgataattg cctggtaata aacaaaaaac aaggcatcat
     5281 tatttctaat gacaccttaa acctaactta ctagaactct ccaaaagaga tttatttttc
     5341 gaattggtac atcttaggca aatctcgttc aaaaacagta aacgggtgat tttcaaattt
     5401 tataaagtct tgaacagcag tttggccagg ataaggcaca aaaaaatgct tcgcatcggc
     5461 attacgccaa accagtatcc aagcaatatt tctggccacg ctatcattga taattggata
     5521 taaaaggttt tttgtccacc aggtagaatc agtctgagca tctaaaccgg tttcggtaag
     5581 agctgaaatt ttattcatag acttagcaag ctctacaaca atccgaagtt gggctatacc
     5641 ttctgatact atgtcttcag atctaaaatt gtaataattg tcaaccccca aaacatctac
     5701 atattcttca cccggaaaac gtttcagata attctctttt gttttgaaaa tatcaggcga
     5761 ataggcataa agaaggttgt ggacaccttt tacatcgcgt aaatacgaga ctgtatagcg
     5821 aaaaagttga atatatttgt catcagtgca acttctggcc ccccaccaaa accaatgtcc
     5881 gttagcttcg tgaaagggcc tgaaaataac agggatcaac ttcccgtttt ttgatttcaa
     5941 ttgactgaaa aaatctgcca ataagtccaa ctgacggtta tatttattat gaaactttcc
     6001 gcccggtaaa atatgtctta ccgccgttgt tgtgtcccag caagagccat ctgtaacagg
     6061 gttttgtagg tgccaactta ctgtatttat acctccacgt tcaaaagcag acttaatcca
     6121 aaacttcatc ctgtcgaacc ggacagaatc aatgttgtat tctccgccaa ttcccgacac
     6181 gtcccatcca taaacagccg gaaaagagcc gcaaacctcg tttacatcag actttaaatc
     6241 ctcatccttc cagccaacac cataagcagt agcgtcttgc tgcccaaaca atatgccttt
     6301 ggaagtattt gcccttaaat tatggaacaa cgctttagtt tcgtatgttg catttgcatc
     6361 aaccgtaata tccgaatctg caggagacga actttgacgg caacctgtca gacacaaaat
     6421 aaatgaagca aatattatct ggtaaaaaga agttgacata gaaagataat tgtaagcaca
     6481 aaagtaagta acagcaatcc gttcaaattg ctataatgtt taaaataggt gaaactatgt
     6541 ttaaaatgaa agaatctggt attacttcga caagcgggta ataagctcaa aacacacacg
     6601 gctgttgtga tatgggcatt tccagaaacc ggccctcatt tcagattcaa taggctttct
     6661 gtctttggta agcttattgt gccattcgcc acgctctttg tcaacaaaat aatcctctat
     6721 aaactgccag ctttttgcag cataatcaag atatttttca tttaccgtga tatcataagc
     6781 atttatgaag ccaacaacag cttcggcctg cggccaccaa tgtttatcgg tatcaacaac
     6841 acctcttgga ttaccttcgt aatactggcc tccatcggca tcaaagctct ctcgtgaagc
     6901 aacatcgaca atatgtagca caattggctc gtacttttct ttcatttcat cagaaccaga
     6961 agctaaaatg gcttcccaca aaagccatga tgtttctata tcgtgaccat acgaaacaag
     7021 ctcgcctttg gcattccaat catcatcaaa gaatagcttg aaatggccat tctcaggatt
     7081 atagaatttt tcggccaaaa gcacgataag gttgtccaaa gcctccttgt attcaggctt
     7141 tttgcaaaca ttataaagat tggtataagc ttccaggata tgtaaatgcg tattgtgggt
     7201 tttgttttcg ttcaggtctt tggcactaat acggacattg tctgtaactg tccagtcacg
     7261 ggcaaaagcc tcaaaatagc cgttttgctt tttatcacgg gcatattttt caagcaaatg
     7321 aaataccttg tctgcctgct ctaaagcaga tgcatcattg gcagccctgt aatattcggt
     7381 aagcccgtaa atatagaatc cctgaacgta acaatatttc tgggtattta taggtttccc
     7441 cgtataatcg agctcccaat aaatgccatt atactccgga tcgcgaaaat atttttccag
     7501 gtattgatat gcccttttgg ccatttcaag atatttagta tctcccagaa cattgtatgc
     7561 ccttgagaaa gtccataaaa tacgggcatt tagcactcct ccctttgaag cctttgggtc
     7621 aagcttccca taaaagtcga tacctccgta aaaacctcca ttctcattgt ccaccatgtt
     7681 gttcatccag taaggcagga tgtttttggt aacctcgtga aatgcagctt ctttcaatat
     7741 attagtccta tcgttgagca tatctttatt attttaagcg tattacaaaa aaacttcctg
     7801 aatataaagt taatatccag gaagttaaac taaaaactta actattactt tttaatgatt
     7861 gaaagatttt tttcaataag ttttttgagc gtagccacag acgaatgtgt gcgatatccg
     7921 tcagcagggg tattcaaaca ataatctact aatttgtcaa ttgtagatac cgccacatgc
     7981 atgcgggtat ctgatgaagc atagtaaata tatacttcgc ccttttcatt gctaatccaa
     8041 ccattagaaa acaggacatt tgaaacatcg cctacacgtt cttcgccttc cggggccata
     8101 aaatgtccgc ctggtgttgc aataactttc caaggttctt taagatcagt catgtacata
     8161 tataacacat atctcaaacc agcagcacag cccctaacgc cgtgggcaag gtgcaaccat
     8221 cctttgctgg ttttaattgg agcagggcct tcgccatttt taacttcttt aatagtatga
     8281 taaacacgag gttcaataat agtctcttct ttaaccacag cattttccat tgagtcaata
     8341 agcgcccagc ctatacctcc accagaaccg gcatcaataa agccatcctg aggacgtgta
     8401 tataaagcat acttgccatt gatgaattca gggtgtagca cgacgttacg ttgctggctt
     8461 ttggttttca ggtcaggcaa acgctcccat tttttgaggt ctttggtacg aacaatacct
     8521 gctttagcca cagcagaaga caagtcgccc gcaggggcat tcgggtcttt acgttctgaa
     8581 cagaaaatac cataaatcca gccatcttca tgcttaacca gacgcatatc atacacatta
     8641 gtctcctctt tgtcagtatc aggaaggtca acagggtaat cccagaacct ccattggtca
     8701 atgccattgg ggctttcagc aacagcaaag aatgactttc tgtcccagcc ttcaacacga
     8761 acaaccaaca aatagcgtcc atccagcagt atagccccag agttgaatgc acaacttacg
     8821 ccaatacgtt gcaataacaa aggattagtt ttataatcca ggtcatatct ccagaatacc
     8881 ggggcatgtt ctcccgtaag gattggatac tgatagcgga catagatacc atttttatct
     8941 tcgcaagcaa cattcttacg catgataaac ttgtcatgtt cggcaaaaag ctgttttact
     9001 ttctcgtcaa attgaacatt agtcatatat ttctctcttt ttaattaatt cggtttgcaa
     9061 atttagcgtt actctctctt tcagaaattt cacctatgta taaaagtata gcacagatgt
     9121 tcattatata aagttaaacc aattgaatta ttaaatttag tgcaaaaatt gacgctaaat
     9181 caattaagtt gctatcaata attcaaaact taacacattg ataatacagt attaacaaac
     9241 aaatctttag cttttgtgac atttgaacta attaaaagat aaacctatgt catttttaca
     9301 tattgattac aaaagataca ttttcttaat gtttatggga tttcaaataa gtatattttg
     9361 ccaagataat ttgaataata acttggaaca acccaaagat tgtaatatac agcaatacca
     9421 aagactatac attaaccgat gaatacaatc aacttatcaa atattgcaaa aaggttggta
     9481 aaaaatcatg gccactattg tttgataaat ttgaaaatgg agattttata gcaatagagg
     9541 caattaaaga tcttacattt gaagagtata aatacgtata tgtaaaagta ataaatgatt
     9601 gcaaaaaaaa agtaaaaaca acccaatctg gtgcttttat tgtcagacct tctaaagcaa
     9661 ttgcaatgag atatataaaa gaactgctgc gaacatttga ttacgattat ataaaaacag
     9721 aagagattat tacatacagc aattctttta aattcagcat cttccccaat cccgtatctt
     9781 ccacaagtat aatttgtttt aacatgcaaa acacagcaaa agtttctgca gagatattag
     9841 acttagaagg taaaaccata tcaatttgca ttaaagaaca aatgttaaac gctggcaaac
     9901 atgaatatcc attaggctta ccctcaaaaa ttaaaggtaa ttttcttgtc aggctttggg
     9961 ttaataatgt tgtaaatgtc caactaatta caaaatatgc aaattgattt aattattaag
    10021 tatctgaaaa gctaatgaaa aaagtattgt caattttgtc caattcctac cttatgaatg
    10081 agcctgaatt agtaaacaaa acaaaatata tacttcttta tatttcaaat aatctattaa
    10141 taaggttata ttgcgtgtgt atgtgtttct tgtattcttg cgaaccagag aacaacagtg
    10201 tgtttaaaat tcatttttct gatggcaaca gaataaccga ttccgatatt atattctatg
    10261 acagttcttc tcattgttta tttttaaaga atgaaataaa aattaaagca aaaacggact
    10321 tttcagttat tgtaaataaa caaacaattt atagaggggt tgttcaatct aaacttgatt
    10381 ctaactctcc attttctcca ttttacatta atgaagtaaa ttctatattt ggcaaaaata
    10441 tcattgagat acaatataat aaaaattatc ctccggattt gagaaatgac ccacagataa
    10501 tcaattccct gaaaagccga aactttttac gtaatggtat tagctgcaat attgatagca
    10561 tagtaataga aaatgctgcc ataggaaacg taaatgtttc atacaattta acaataatca
    10621 ataatgataa ttacaattat tacgtaatta ataaaggatt tgttgatgtt atgttcatga
    10681 atattgacac ttacgcttta tggtatttaa agtcaaatcc cactcaggaa gaaatgccag
    10741 tttataccct aaataacttt tcccttctta aaagtaactc aaaattaacc cgaacagtta
    10801 aatttttaaa ctcttataga atggagccag gatattatca agttacttgc acaattcttc
    10861 atggaggatt tgaaaactta agtataaatc aggatagtgg gcgcatttgg ttaggaaaag
    10921 ccatatcggt aatagatagc ctaaggatag attgaagcct gttccaaaca taaatttata
    10981 ctattaaaac attgcaagga ttaaaaggct gtatattctt ttcacgatga aaaaactgtg
    11041 attcaaaaac acaataacag aaatagacct gaaaaatttt acctttgcag cttgataatt
    11101 tgcaattgaa gatggaaaaa aagattgacc agaaatttac tcctttaaag gaacttggcg
    11161 agtttggcct gataaaccat ttgaccaaag atgttattat tcaccagcca aatactgtaa
    11221 aaggaattgg agatgatgct gctgtccttg atactggtac tgtttaccag gttatcacta
    11281 cagacttact ggcagaaggc attcatttca acctggttta tactccgttg aaacacctgg
    11341 gatataaggc tgtggcagtg aacctgtctg acatttgtgc catgaatgcc aagccaaagc
    11401 agattacggt tgctttagcc atttcttcaa agttttctgt cgagcacctc aacgaacttt
    11461 attcgggcat caagcttgcc tgccaaaaat atggcgttga ccttattggc ggcgacacta
    11521 catcatcatt aaccgggcta accatctgca ttacagccat tggcgaagta gaaaaagata
    11581 aagtaaccta tcgcaacggg gcaaaaaata caaacctgct ttgtgtaagt ggcaatgttg
    11641 gtgcggcata tctgggattg caaattctcg agcgtgagcg caaattgttt gaaaaagaag
    11701 ggataacaca accagacttt ggcaactata cctatttaat tgaaagacag ctcaaacccg
    11761 agcctcgcac agacattgtc aaatggcttg aagagaaaaa cattgtccct actgctatga
    11821 ttgacatttc agatggtttg tccagcgaaa ttcttcatat atgtaaatca tccaacaccg
    11881 gatgcaggat ttaccaggaa aaaattccat tgcatgagca aacagccgat tttgccgaag
    11941 aagtaaacat gcacccaata aatgccgccc ttaatggtgg tgaagactac gaactactgt
    12001 ttacagttga tatcaagcat tacgaagcat tgctgcaaaa tgaccaaata cgcatcattg
    12061 ggcacataac agagcaagta gaagggacaa accttgtatt agaaaacaac gaattcgtca
    12121 acctggcctc aggcggttgg aatgctctta attaaaaaca tttctaaaat tctaaattct
    12181 cttaaaaata cttcgactcc gctcagtatg accaaattac gtcacattga gcggagtcga
    12241 aatgtttcaa accttaaaat ctttcgtatt ccgagtcttt tccggcatcg tcatcaaaca
    12301 tttttaagtt aaagccggaa ttctttgttt caggttttgt tggttctttt tttgaggtaa
    12361 ctttcagggc tgcaggtttt tctgcaacag gggtattttc gatagcttta tctttttcgg
    12421 ctataggctt ttcttcttct atgccaatta cagaactgcg gatatctgat tttaactttt
    12481 caaggcgttc atgccttgca gaagctattt catcaagttt cttatcaatc tttttgataa
    12541 tcttaggttc ttcttttttt atttcttttt tcaattcctc ttcaactttg gctttctttt
    12601 tagagtcgag cctaaagaat gtagtgattt ctttaaggtt ttcagattct ttggtaagct
    12661 caatagcgct ttgggctaat gaatctgaag atacagcatt ttgctgtata acatggttca
    12721 agcgttgaag ggcattgtta atctgctctg cccccgcatt ttgctcaata ctggcagcag
    12781 taatatcctg aaccagtttt gctgtttttt caatctcggg ggcaatagaa ttgaggttca
    12841 tggctgctgt ttcagatata gttactcctt gtcttgaaag ttcgttgatt tggtctgcag
    12901 caactttgct atgttcggct aattttcgca cctcagcagc tacaaccgca aaaccacggc
    12961 catgttcgcc ggcacgagca gcttctactg cagcattaag agctaatata ttagtctgga
    13021 aagcgatatc gccaataaca gaaatctttt cagcaatctt tttcatcgaa tcagcagcag
    13081 taataacaga ctcattaccc ttctttatgc cattagcaga ctctgtagct attttttcgg
    13141 tttgcttaga gttatcggta ttttgctgaa tattagctac catttcttcc atgctcgatg
    13201 aaacctcctg ggctgaagct gcctgatctg aagccccaga agataattgg tctgctcctt
    13261 gttttatttt gtcacttaaa tttactaatg agtcagagct ttttaggata ttctcagcta
    13321 tttgttgtaa ttgtgctgta agtttttcaa tggatttggc aatatctcca atttcatttt
    13381 cctgtgccgc aatattttta tctacttcta ttttgatatt cccattggta agctcatcaa
    13441 tattttcttt caaccctata attggctttt gtataatttt aattaacatt atattactgc
    13501 tgagcaacca aataatcatg acaggtaata cccaaattaa agctttcaac cctaactggc
    13561 cattgacaaa ggcaagaaaa gccacaccgg ccgatacaac atatacaact ctggcaattt
    13621 tggcaacaac agttttttta aaaagcaaat gaataacaac tgttgctacc gggaaacttc
    13681 ctaataatat caaaagaaaa actaaaatcg gactcatgtt tgcgaaattt tttcttagtt
    13741 aaaaatcagt tcaaaaacat tttagctgtt ttacgacaac taacatttta agttttaaat
    13801 ttactaaata tattcttata aaaagaaaat atcttaatta aatatggcca aactaccgtc
    13861 aattaatcaa atatacgcaa ttttttctga aagaaaccat tgaatataaa gcagtttatt
    13921 gtaaatttac cataaacgcc ccctcggatg aaaaaatggt tttttaaaat tatcccttca
    13981 gaagaatggc actttactgc cattgtaacg cttggaattg tttttggcct tggtttttat
    14041 attttttata tttcaaaagc ctggtcatac ctgtctgaca gacccgagac ttgcgtaaac
    14101 tgccatgtta tggctactga atatgttaca tggtttcaca gttcgcacaa gagaggtgtt
    14161 tgcaacgact gtcatgttcc tcatcaaaac tttttggtta ggtacctttt caaagcaaaa
    14221 gatggcttgc gccatgccac tattttcaca ctcaggcttg agcctcaaac cataaaaatg
    14281 aatgaagcag gagttgaagt tgtaggcgaa aactgcctga ggtgccataa agctgttttt
    14341 ggcaggttgt ttcaaaatgc caaacaaacc caggaaataa aaagccgaga ctgttggaat
    14401 tgtcataggg aaacgcccca tggaagggta aaaagcttat cgtctgtgcc taatgccagg
    14461 gttccactgc ctgaaagccc tgttccggaa tggttgagcc atttattgaa gaactaaacc
    14521 aaaataaata atatgaaagc tgtaaaccaa ataatcaaag aaaagccctg gatgggatgg
    14581 gttatatttt tcattaccat ggtagttact ttttttatag gcttattggc atcctccatc
    14641 atggaacgtc gtaccgaagc aattataata aacaaaccta aagctgacat taaagctttt
    14701 gaaagcgaca acaaagtttg ggggcaatat tataaagatg agtatcagag ctatctgcag
    14761 accgccgaca ccagctttaa aagcaaatat ggtggaagtg caaccattga tatgttagaa
    14821 gaagctccgg aaatggtcgt cctgtgggct ggttatcctt ttagcaagga ttacaggcaa
    14881 ggacgcgggc actattatgc cgtggacgac attattacta cacttagaac tgctgctcct
    14941 gcaaagccag gtacagaccc tatgcccgcc acatgctgga catgcaaaag cccggatgtt
    15001 cccaggttaa tgaataaaat gggtattgat agtttttatc atcaaaaatg gtccactttc
    15061 ataacagaaa tcaaaaaccc cattggctgt gcagactgcc atgacccggc tacaatggat
    15121 cttcaaatta cccgtccggc gctgctcgaa gctttcaaac gaaatggcaa agacattgcc
    15181 aaagttagcc aacaagagat gcgaagcctc gtttgcgccc aatgccatgt tgagtattac
    15241 tttaaaggtg atggaaaata cctgacattt ccgtgggaca aaggaatgga cgttgataac
    15301 attgaaaaat attacgatgc tatagaattt tcagactgga cacacagcat cagcaaagca
    15361 aacatgatta aagcccagca ccctgactat gaaatatatt cgatgggcat acatgcccaa
    15421 aggggattgg cttgtgctga ttgtcacatg ccttataaga gtgaaggcgg aagaaaattc
    15481 actgaccacc atatacaaag cccgctcaac aacatttcta acacttgcca gatatgccac
    15541 cgcgaaagtg aggcagaact actaagaaca gtatatgaac gtcaggataa aattgccgaa
    15601 caaaaacaaa tccttgaaaa actgttggta aaagcccatt acgaagctaa ggcagcatgg
    15661 gacaatggcg ctacagcgac agacatgaaa cctgccttaa ccgaaatcag gcatgcccaa
    15721 tggagatggg actttgcagc agccagtcat ggtgcagctt ttcacgcccc tattgaagtt
    15781 atgcgtatta tagctacagg tattgaaaaa acacagcaag ccaggcttat tttgtcaaaa
    15841 atatcagcta aaaaaggcat catagaagat attgctattc cggatattag caccaaagct
    15901 aatgcacaga aagcagttgg gcttgacatg cccactatag aaaaagaaaa acaattattt
    15961 ttgaagaaat taaagaccga ttggaaatag tttttgaaag tttgtaacca attacccgga
    16021 ggcaaaggga gcaatttacg agacactacc gaacgtggtt tgtgatacgg cagattgcgc
    16081 ttcgttcgtc tgaggtttca gcttcagaca cccttaatag actacagcct gataaaatac
    16141 ttatggctat ggcagagcgt tagccgaacg gaggcactaa cagccactga ggccaacaaa
    16201 aacaacaacg gtattgactc caccgatgag ctgcgcgaat acgtgagtgt acaggaagcc
    16261 aaggccagcg ggagcataca acatcctgct gtggataggg ataatattta ccagaagttt
    16321 gggtttccgt tgaagtaaca agtaataaat aaaaaagcct ttcggtgaca aaaaatctga
    16381 aaggcttttg gttttataac agaaaaataa ttttatctta tttcaaaaac agtgccactg
    16441 ttcaagaggt catctacaca tttgatgtct gagcttttct tggccgactc tatttgttca
    16501 taaaaaaggt cgtcataggt atcacgcttt gcagccctga taatgccaac tgcaacaggg
    16561 aaatgaggag gggccatttc tattagcata atctgtaagc cgggattgtc atcgtacata
    16621 tcatgaacaa gtatgtcgct ttcttttacg ccatctttac caacctcaac aactaacaat
    16681 ttaccatttt tgaacatgag tccttttttc atgtttttac caaaaagcat tggcttgcca
    16741 tgctcaagca ctaacatgtt ttcttcacgg gcttctttgc ctacccatgc ttcgtgagcc
    16801 ttatcattaa agataataca gttttgaagt acctcaacaa ctgaggtgcc gtcatgttta
    16861 gctgcagcat atactgcttc gcctaataaa tgagcattgt tgtcaggaac acgggcaaag
    16921 aaagagccac caactccaag cactaatttg ccagggttta caggaggttc aatagtgcca
    16981 aaaggtgatg tttttgtaac agagcccatt ggcgaagtag gcgaaaactg tcctttagta
    17041 agcccatata tttggttgtt gaacaatata atatttaaat cgatgttacg acgaacggcg
    17101 tgcataaagt ggttgcctcc aatggcaagc gagtcgccat cgccagtagc aacccataca
    17161 ctaagaccag gattggcgag ctttacacca gttgcaatag cagaagccct cccatgaatg
    17221 ccatgaaaac catacgtgtt tatataataa gggaaacgtg aagaacagcc tattcctgaa
    17281 acaaaaacaa acttatcttg gttgtaccca attttaggga atgtattgag aatagaagcc
    17341 aggataacat gagctccaca accggggcac cattttacca tctggccact ttgaaagtct
    17401 tctttagtaa gctctttgct tgatttttcg atagaattat atattgcatc cattatttct
    17461 tttcctttaa cgtcttacaa taacttgctg ataacatttt taatttcggt ttcggtaaaa
    17521 ggcagccctt gtactttgtt gtattgctcg tactgaaaag caggatgggc catacggagg
    17581 taattcacaa actgtcccat attttgctcg gcaacaatga tttttttgaa attgttaaaa
    17641 atttcatcca cattggttgg caaaggcatg atgtagttga aatgggcatg gcctactttc
    17701 ttcccatcgg ctgccataga ttcaactacg gtttgtataa ccccttcaga actaccccat
    17761 gaaacaatta gcaaatcccc ttgttgagct cctgttactg tctgttttgg gatacgtgaa
    17821 gatattttgt ttaccttctc tgcccttaat ttgaccatca gctcgtgatt agcggcatca
    17881 gtagtaacaa ctccggtaac attttccttt tcgaggcccc caatacggtg gcgcaaaccc
    17941 ggggtacccg gaaatgccca gcggcgaaca agtgtgtcgg ggttgcggcg ataaggttta
    18001 taatcgggtt cgtttggcgc tgcaattgga ggatttatga caggcaaatc ggccattttg
    18061 ggtatcctga aaagctgaga accattgcct aagaaaccgt ctgttaaaag gataacaggg
    18121 gtcatgtgtt ctaatgcaat acgggcagct tcaaaagcat aataaaagca gttggctgaa
    18181 gaagatgctg caattacagg aatgggggca tcgccattac ggctaaacaa tgcctgatat
    18241 aagtcgcttt gctccgactt ggtaggcatg ccagttgaag gccccgccct ctgtacgtca
    18301 atgataacaa gtgggagttc agtaattaca gctaacccca tagcttctac ttttaatgaa
    18361 agccctggcc cggaggtagt tgtaactgca aggttgccgg caaaggccgc accaatagcc
    18421 gatgtaatac cggcaatttc atcttctgcc tgaaaaacct tagctcctaa tgatttatgt
    18481 ttgcttaatt caactaaaat atctgtagcc ggcgtgattg ggtacgaacc gaggtacaaa
    18541 ggcaaattgg ccttctcggc agcagctaac aatccccagg ctgcagctac attaccagtg
    18601 atattgcggt atttgccttt ttcgagtttt gctggtgaaa cctggaatgt agaatccagc
    18661 gcttcaatag tatctgcaaa atcataacca gcctgaagta cctttttgtt agcttcaagc
    18721 atttctcctt tttcagaaaa cttatctgac aaatattcaa ttgtgtgctg caaatcgcgg
    18781 ttgaacaaat aatatatcat gccaagggca aacatatttt tggttttgtc cacctgcttt
    18841 ttgttgagcc ctgaatctgc caaagctccc ctggtcaggg tagtaatagg ggctttaata
    18901 aggttgtagc cttccaggct tttgtcttca agcggatttg acaggtaacc tgcttttttt
    18961 atggcttctt cgtcaaatgc atcaacatca acaatgatag tagcaccacg ttcggctact
    19021 ttcaggtttg ctttgagcga tgcagggttc atggccacaa gtacatggca taagtcccca
    19081 aaagaattga ctttgctttt gccaatgtgt acctgaaagc ccgatactcc ggctactgta
    19141 ttgtgcgggg ccctgatttc ggctgggtaa tccgggaaag tggccagatc attaccaaat
    19201 accgcagcgc tatcagagaa aagtgtccct gtaagctgca tcccatcacc cgagtcgcca
    19261 acaaacttaa caactacgtt gtctaattcc ttaacttttg tctttttagt cattttcaac
    19321 ctctctggaa atccaccaat taaaaaatta tgaatattta tacaatacgt gtgaataatt
    19381 atagcatgca aaggtattaa aaaaattaaa gtagcaaacc ttttcagggg ttttttgtta
    19441 aaaaaataac agtagtaaac tcaatgtcgt ttagttaatt attaaaaaaa acgccggcag
    19501 gtctgaaaga cgctgtcggg ttagtaccac tctgacctga cagcgtttgc aaaacctgtc
    19561 agagtcttga ctaaacgata ttggtagtaa attctttaaa accatcttcg caaaatactg
    19621 ctgtaaaagg ttttgctatt ttcgtttttc taatgaaacg agcatgtttc gttaaacaca
    19681 aacaaggata aatcccgaat cgaatagtcg ggacgagttt tctttcgcat agcttttcgg
    19741 ggttcatctg accttgataa aaaaattatt tacggatgaa ataattaacc attccttaat
    19801 atttagcttt ttttgaggta gcattagagg cttttctttg ctctataact aatcttaaaa
    19861 aagatgataa tggattcaaa caacccaatg cctaatttca gggagcttga acaaaacagc
    19921 ctcgaaaaag catttttgaa aaaccttaac catgaattgc gtaccccaat gaatgctatc
    19981 attggctttg ccaacctcat ccaaaaccat tgtcatggag ataaagaaat agctgaactt
    20041 gtagaagcta tgaatttctc atgttttcaa ctcctaaatc ttgttaatga tttggttgac
    20101 ttgtcaaaac ttgaaaccaa taagcttcat ctacacccgg taaaatgcga ccttaacgaa
    20161 ctaatggaat cgctttacat tgattatcaa aaacaactgg ataaagataa taagcctatt
    20221 gaacttttaa tatataaaga cgattgctat agagatatat ttatcgatat agaccgggca
    20281 aggtttactc aaatatggaa atatatcata gataatgcca tgaaatttac gcatataggt
    20341 aaaattgaat ttggctatat gataagaggc cggggagtgc attgctatgt gaaagataca
    20401 gggataggaa tgccccaatc gtcagtaaaa atggtatttg agcaatttgt ccgaaatagc
    20461 aattgtgaat atggaggact gggcattggg cttactattg ccagaaaact cattgaactt
    20521 atggaaggac atatttggat tgactcagaa caaggcaaag gtactattgt atattttaca
    20581 gtgcctgtag aacccgaaaa caaaaaaaag aaaaaagatg aacttattga taaacttatc
    20641 attattgacc aaaaacaatt gcaataacct ataaaatggt tttttgataa aaaggtgtgt
    20701 tattcctccc ggctaattta caattagtta atactccgtt aacgcttaca aaatatacca
    20761 aggatacttt taccacagca aaaaatgtat aaccaaattt tattttttat gaaaaaaaca
    20821 ttactcgtat caattttagc cattgcttct gttattggag ccaatgctca aagtattaac
    20881 gaccctttct ttgaaaaagt aagctatgtt ggcgcttttg gaaatgaaga ctggacaaaa
    20941 ggctgggcaa attttgaccc acaaaataca gattatcctg aaactacaac tacacttggc
    21001 aatggagaac tttcttacgc tgccggatat aaaattacaa gcaacacaag tattagtggc
    21061 gttgtaaaac ttagtggttg ggtttatgtc aaagatggcg ctacccttac aatagaaaaa
    21121 ggaactataa taaggggtga gtcttcggct gctttgatag tagaaagagg gggcaaactt
    21181 attgccgagg gaactgtcga taaacctatc gtatttacat caatcaaacc aaaaggttca
    21241 agagctgcca gtgactgggc cggtgtaatt atttgcggaa aagcaaccaa taacaaatct
    21301 aacaataccc tgattgaagg tggtgtaggt gcttattttg gtgctggtga ccaggcagtt
    21361 gaaaatgaca attcgggcat attgaaatat gttcgtattg agtttcccgg gtatgatatt
    21421 gatggcgccg gcaacgaaat caatggactt actatgggta gtgttggtag cggaacaaca
    21481 attgactatg ttcaggtttc atttagtgga gacgatgctt ttgaatggtt tggcggaagc
    21541 gtaaattgca aacacctcat tgcattggct actgaagatg atgactttga tacagacaat
    21601 ggatatgtag gaaaggtgca atttgcttta gctgtccgtg acccaaaagt atgcgatacc
    21661 gatggcgctc gtggttttga atcagacaat gatgctaact caagctataa caagccttac
    21721 actgccgcag tttttagtaa tgtcagcctt tttgggccaa aaacagatga atctgccaat
    21781 caaaagcatg atgtagctat gttacttcgc cgtaacaccc gcttgcagat atacaacctg
    21841 ttctcaaccg gttttgtaaa aggcggtgta gttattgatg gtgatgtaac ccaaaaagct
    21901 gcgggtgaag actctttggt gattaagaac agtatttttg ccggatacag caagtttatc
    21961 tctacaaaag ggaccaataa ccctttaagc aatgctaaag catggttagt taatggtaaa
    22021 aatgatactt taaacaacct cgataatgta aaagtacaat ttccaatagt agtaacagct
    22081 cctgtgttta tccccgaatc aggttctgtg ctcttgacta aaggttcttc atttggcgct
    22141 gctgtaacat ctatagttat aagtgctgct ggcaacgtta catccattaa aactactgaa
    22201 acacttcagc tttcggctac agtgacccca gacaatgcag tatctaaaga agttagctgg
    22261 agtattgaat ctggaagcga cgttgcttcg gtaagcgaaa gcggattagt aacccctcta
    22321 aaagcaggta atgtgaccat caaagctgtt gctaaagacg gttcaggtat aagttcaacg
    22381 tttgccttag aagtaaaaag caatgttgct gttggaagta catctgttgc aacagcaagc
    22441 atctggccta acccggcaat ggatttggtt caggttaaat gcgaaaacga aatttcttca
    22501 ttggaagtat tgaacaatgt tggacaaatt gtattgaaag tagctaatat taacagtaac
    22561 aactccagtg caaatattag taacctcaac agcgggatat atttcatgaa attgactatg
    22621 agcaatggcg ctgtaagtgt aaacaagata gtaaaaaaat aactctattc ccttcacatt
    22681 tcattgaaaa ggttgtcctt tcggggataa cctttttatt tatataatat ggacagcata
    22741 agcaaagcat aaaaaaatta ttttaaaagg aatgatagcc tgcacccttt aataaatttc
    22801 tttgtgttta ttctctatat acatgatgtc acttagctta gggttgaaaa gccaagggtg
    22861 tttcaaactt taattttctg tatttttaat tccaaaaaac tttttcttca gccttaaaaa
    22921 ccttaacttt agttttacac taaaccaaac aggtatggct gaaaacacaa atattcagtt
    22981 ggagctaaga aagtttgtag cccccgagtt tatttttggg cttgatgcac gcatgttagc
    23041 cggaagatat gtaaaaaaac ttggggcaaa aaaagttttg gtagttacag atgccggaat
    23101 tcatacagcc ggttgggcca atgacgttct cgaaacgttg gatgatgaaa agctttctta
    23161 tcaaatattt tctgaagtca gccccaatcc tcgttcagcc gaggtgatga aaggcgcaga
    23221 ggtttacaaa aactccaatt gtgatgctat tgttgccatt ggagggggta gtgtaatgga
    23281 ctgtgccaaa ggtattggca ttgtatcttc aaatgataaa aatatccttc agtatgaagg
    23341 agttgataaa gttgatattc caattccccc gctggtatgc attcctacca ctgctggaac
    23401 atcggccgat gtatcgcagt ttgccattat aaacaatgag catgacaagg tcaaaattgc
    23461 cattatcagt aaagccgttg tgccagacgt ggcattgata gacccggctg tacttgttac
    23521 aatggattca tacctgaccg catgtactgg catggatgct ctcgttcatg ccatcgaggc
    23581 gtttgtttcc actgccagct cgcctttcac cgacctcaat gctttagaag ccattcggtt
    23641 gataaacgag aatttggttt cttctatcaa tcttccaaat gattatgaat tgcgtggcaa
    23701 ggtgatgctg ggtagtttgt atgccggatt ggctttttcc aatgccagtt tggggtgtgt
    23761 gcatgctatg gctcacagtc ttggtggttt tcttgatttg cctcatggcg aatgcaacgc
    23821 cattctgctt cctcatgtta tgagctacaa ttttgaatct gctacggcta agtttgcgaa
    23881 gataggagag gtgcttggcc ttgaaatgaa aggcctttta atgaatgaaa agcgaaaaat
    23941 gctgattgaa catatcatgc agcttaagac aaaaactggt attctaaaaa cacttaggga
    24001 aagaggggtt ggcagttcag atataccgtt gctggcatca aaagcaatta aggacccgtg
    24061 tcagataacc aatccgcgta ctccgtcaaa agaggatatt gaagttattt atagtgaagc
    24121 tatgtaatat gggcgatttg caggatagag attctttgag ggataaaatc attggcttgg
    24181 gtgaaaactc agcccgtaaa agctattacc ctgagctcca aaaaaagatt aaagaactgg
    24241 aggagaggga gcaaagcctg tatgatttga ttaacaatat cttcgatgcc attattattc
    24301 acgatgaaga aggaactgta ctggctgtaa atgaagggat gctggcgatg tatcgtatgc
    24361 ctgataagga aaccgccctt caatatacga ttatggacta ttcttttggg tacgagcctg
    24421 atgtagtgaa agaagtgttt aaaaaaacct taggaggcaa gcctcaaatt tttgaatgga
    24481 gagcacgtcg tccgctggat aatagctcgt ttgaatctga agtatctgcc agaactgcaa
    24541 cctggcaggg caaaaaggtc attattgcta ctgtccggga tattaccgaa cgtctgcaga
    24601 cgcagcttaa actgattgag agagaacaac aactggaatg ccagaatgaa gagctgattt
    24661 cgctcaatga agaacttgca gaacagaatg tacgaataga acagtttaat gagcagttga
    24721 aaatagctaa taataaggct gttgagagcg accgcctgaa aacggccttt ttggccaaca
    24781 tgagccatga aatccgtacc ccgttgaacg gtattcttgg ctttaccagc ttgttaccag
    24841 aagcgagaaa ctctgatgaa gcgtttgaga agtacatttc tattattcaa acctgttcta
    24901 atcaactact tacactaatt gatgatatta tagacctttc aaaaattgag gcaggcatga
    24961 tgaatttcaa ccctcagttg ctcaatatca ataatttcat gcaacaatta tgtgcttatt
    25021 acaagagtac accctctaag gctgaattaa tctgcgatat cgaaagtcga aacgatgaat
    25081 tgaccatttt tgttgatcag gagcggttgc gccaagtttt taataactta ttgaataatg
    25141 ctataaaatt taccagttct ggtaaaatta catttgggta tcatattaaa aatcaagagg
    25201 ttgaatttta tgtttctgac actggcattg gcattgctcc tgaaaatacc gaaattattt
    25261 ttgaacattt caggcaggtt gatgttggtt ttaccaagaa atatagaggg actggtttag
    25321 gattggctat ttgcaaggcc ttattagaat tgatgggagg caggatttgg gttgaatctg
    25381 ttttggacaa aggttctact ttttatttta ccgtgccact ttcttttgaa aaagaggacg
    25441 ccaatgttcc tgaagctaat aaagcatttt cagttgaagc gttaaaaggg aagaccattt
    25501 tagttgcaga agatgaagag tttaattata tgtttatcaa tgaattgctc tctaaggctg
    25561 atgctaagct cattcatgtc cttgatgggg ccaaggcagt tgagacggtg aagcagaacc
    25621 ccagcattga cctgatattg atggatatta aaatgcctgt tatgaatggt atagatgcta
    25681 cccggataat aaaaaagacg tatcctcaaa tacctgttat tgctgttact gcgtatgcta
    25741 tggctgatga caaagaaaaa gccctggagg ctggttgtga cggatatttg gccaaaccaa
    25801 tcaacaaggc tgctttgttt gcggctatca atcagtgtat taaataaaat agatttttat
    25861 ttagaaaaat acttttctgc aaactttata aacgttggcc aattgggaat aactgtgtgc
    25921 cctccggcat gctgtctgaa agctatatcc ccgtcaatta aagggggtta aataggaggg
    25981 aaagttgtgg tgcttatatt agtgaattgt aaatttttat tttattatta tgaaataaaa
    26041 attataatta tagtatttaa taaaaagtta tagtatattt gtatgtaaat ttttgattat
    26101 gaaagaaatt accaaagctc aggaagatat attacaagcc ctttggcaaa ttaatgatgg
    26161 cgcagttagc gatgtgcttg acattttgcc ggaaccaaaa cctgcataca acacggttgc
    26221 gacggtgatt aaagtgttgg agaaaaaagg ctacgtttcg cataaaacct atggtaagac
    26281 taatgtttat taccctttgg tttcaaagca ggactatgcc cgcaatgttt tgaaaaactc
    26341 agtaaaaggt ttgtttaacg ggtcattcaa acaggctgta tcgttctttg tcagggaaaa
    26401 caaggaagtt agcttggcag agctcgaaga actcaggaat tatatagaag aagagattaa
    26461 gaataagaaa aactaagcca tggaaagttt ttgtattatt tagccgggtc atccctttgc
    26521 cttaccctgt tttattgcgt ctattatttt cttttcagta aagaaagcgg acacaggttt
    26581 gttcgtttct acctgctttt ttctgtgctg gtatcattgc ttttgccact tagtagttat
    26641 tctattcgtc taaataatgt atatatagaa actccaaaaa ctgaaagcgc tataaaatct
    26701 gatataaaaa acgataatgt tgcaatttct cctattaaag aggaagtttc agcagtatat
    26761 cattctccag ataaacctgt gaattattgg gttattgttg tttgggctta ttggctgata
    26821 tctgcagtgg ttttcttata ttttattatt ggaattatca atatttactg gcattacaga
    26881 caatctaaaa aggaacaaat cgggaatgtt gttattttaa gaaatgaaag cctaagtaat
    26941 tcattctctt ttatgaattt aattttcctg aacccaaacg ggctttcaga aaaagagaca
    27001 gatagcattg tagcacacga aaatattcat gtttcgcaat accataccat tgacttattg
    27061 ttgattgagc tcgtagcggc cgtaatgtgg ttcaatcctt ttgtgtggtt gtttcggaaa
    27121 tcatttaggc aggttcatga atacctggct gatgaaggag tactcaatat cggttttgac
    27181 aggctcgaat atcaagcact cctaatcaat caggttgccc agggaaggct gttaagcctt
    27241 gcttgtaact ttaatcaatc actaataaaa aagcgtatta tgatgatgac aaaaattaaa
    27301 tcgggcaaca aagcaaaatt aaaacttttg gctattgttc cgctcacttt agggttattt
    27361 ctgatgattg cctgtgccaa tgggcacaat ataaataaag taactgctgt agaaacggtt
    27421 cgtatgaatg tgctttacct tggagttgat aaccctataa aaattgcagc ttcgggttat
    27481 gatccttcgg aacttgatgt aacaattgac aatggaaaaa ttttcggtaa gaatggacag
    27541 tatatagtgc gtccggaaaa agatgggcag gcttggctta ctgttagttg taaagggaag
    27601 gttgttcagt tgacatcgtt tagagtgaaa attgttcctg acccggcagc aatgattcca
    27661 gttcaagaac aatctggata tctttttaga atgctgaaga gtggagaaat ttcaaaaaag
    27721 gaattacttt ccgctacagg cctaatggcc ggaatggaga atttcgattt tgatatagaa
    27781 tttaaggttg cgtcttttgt attgagtgtt acagtcccta attctatgga ggttaaagaa
    27841 gaaatttcca actcggagaa gttttcgaaa ggtcaaattg atttaatcaa atcattggtt
    27901 gtaaaccaaa aacttacagt tgagaatatt actgttgttg ggtctgatgg caccaaaaga
    27961 aaactaagtc ctttagtctt tactattgtg gctgataaat aaattctata aacgctttgc
    28021 ttttctgaaa aagcaaagcg ttttacttcg ggtatcctac ggggtgcgca attataaccc
    28081 tttgcctgct agaaagatga agtaaactat cgattttagg cctgttaatc cacccaacag
    28141 caacagtagc aaggttttcg gatgcacaat acagatagat gttctgactg atatagcctg
    28201 catctatggt ttcataatca tttcgcgtgg tatcgccttc aaaggcttta ttcaaatcgg
    28261 caacaagaat aagagtaagc ggtacaatct tatgaaaatc ctggtcacca atttcggcac
    28321 ggatgtcttt atctgattgc ttaataagtt tttggttgtc agcgtcaaaa aggtaaaccc
    28381 ccgatttcat cgctacataa atgtccaact cttgtctgtt ccttgcagtt ggggctgtat
    28441 gccttttttc ggccggccgg tttatgccat tggcagccca cagcaggtct gaaatttgct
    28501 gtaatgacag ctctttgtca gaaaagtcgc gggttgtttt tctatcacgc aaagcaagca
    28561 taagaggctt gccgcctttg atttgtggcg ctggcaaatt gtcaatactg gccgatggct
    28621 gctgattgtt agtgcacgaa atagctgtaa atattacaac tgtggcaaat agttttataa
    28681 atttcatgat ttgtttgatt tataaattaa agtatttcta tttcttggtt cttgactctt
    28741 gattctttat tctttaatta gcctcttttc ttccaaatac acctttgccg tatcttttac
    28801 agattgggct attggagtaa atttaaaccg caaagtatcg gtaatcttct tattagaata
    28861 ggcatgagaa ctgaagccac tatctactgt ttcgcgggtt atttgtggtg ctttccctgt
    28921 aattttcgac aaaatccaga tggcacgcca tccaaactca gccatccagc gacttacctc
    28981 aatggaaggg gcttttttgc ccaatgaatg ggcgacaagg ctgaagaaat ctttgtaact
    29041 caggttttca gagtttaata taaaacgttc ccctttaatg gacgaattgg taagcataat
    29101 catgacattt accacgtcac gtacatctac aaatcctgat gcgcccgggg tatagaatct
    29161 cattccttta tacacggcat caaacatggc tgtgctgctt ttgccccata ttccggggcc
    29221 aacaataata gaagggttaa ctattacagc gctaaggcct tcttcaattc cccgccaaac
    29281 ctccatttcg gcatggaact tacttaccga atacgtcgaa cgctctttag atggtgtcca
    29341 tatcatttct tcgttgataa gctcgccatt gccagcgtgg cctattgctg caacagaact
    29401 catataacac aagcgtttca cccctttatt aaggcaggca tttactacat tggcagtacc
    29461 attgatgtta ttttccagca tccgtcgttt cccagacgag gaaaatgaca cctgtgccgc
    29521 acagtgatag acagtggaag ctccttcgag tatttcttca agaatagtaa ggttttcaat
    29581 gtcgccctgc acccattcga tgtgtcgcga aagcttttca atatcatcat agtagaaaga
    29641 aaagatacgg taagcattat cgatgctttc tttgttgcgg tacatggctt ttaccatgtt
    29701 gccgttgacg gcaagttcat aaataaggtg tgccccaacc aagcctgtgc ctcccaatac
    29761 tactatcatg gtttaatatt ttaatgtcag ttttacaaaa attgatataa tataagtact
    29821 ataacatcag ttcgatataa gagaagctga tattaaaggc tttaaatgta atagccttac
    29881 ttttgcctta aagcaaaagt aacaaaaatt caaggcttgc cgtaaattgg ctaaaaaaag
    29941 ctttatcgac ggctccgggc aacaactcgc ttcgctcaaa cagttgccct cacttcgccg
    30001 ataaagcttt tttcttaacg ccatttacgt caggccaata gtagaaaaga ttcaatgaaa
    30061 acggcgatac ctttaacaga aagattccgt ttcatcgcct ttttgtttga tttcggccga
    30121 gaagcctaaa gcttctattg tttgcatatt gcaacattac aaggcttcga gggtggaaga
    30181 gttaagacga taagagtttt tgagcaactt ttttcgacaa aaaagttgca gataaataaa
    30241 gcaagcattt tcaagccata tataacaatg ctttaatcag gttttattta aaaaaacttt
    30301 taattaacct ttctgccagg tcagcttttt gccgaagccc agcgtattgt cggtaaattt
    30361 aataaattct ggttcaaaag cccataagtt caggtccata acagaggcat acggaaaccg
    30421 cttcaaatat gcttttgaaa gttgtaagcc ctgagtgtct actattatct tgccagacat
    30481 ctgaaccccc tgaagtttgc ctataatttt ggtttctaaa gctatggcta ctgaaactat
    30541 ttcattcttc tcaaattcct taacatgacg tgtttctggc gaagaagaga atataagttg
    30601 caatgggtcc tctgaaacag catagaaaca gctgcaactc cagggaatgt tgttctggca
    30661 tgtagccagg gtaagcacat ggtgcttttt gataaatgaa actatttttt tatctggcaa
    30721 cacaatattt tgtccttctg aatggctcat ttttctctct ttttcatttt cccgaataag
    30781 taatttcttt atcattaagg ttaatggcta tcatttcccg tgcaatcagg ttaattaaag
    30841 catcttctgt ttgacgtata gtcaaatgca acaggcgcga aagttttgaa atggagacat
    30901 gtttatagtc gcttatattg gcaagtatca attgttctgt ttctgtaact ttagataatt
    30961 taatgttttt gtttcgctta attttttgtg cttctacata aacgcggttt gcaactatat
    31021 tttggtcatt aatccgaaca atgacaatcc atttgttgtt ttcgtctttg gcgtagcaga
    31081 gttttggatt tttctcaaca atgacttcaa gcactgtctt ttgttctatg agccagggcc
    31141 tgaccacaat gtgaatttcg ggtttgcagt acatcgaggc tgcggcttct atcatataaa
    31201 attcttcttc agacctgaca cctgcaatgg caccattgtc tttaacgcca atcaataaac
    31261 gccctccttg cgtattggca aaagctacaa gggtacgggc tatttttcgc gaattgctga
    31321 tttcgaattt aaaatcgaga gtagtatgtt ctccttcact aataagcttt ttgatatatg
    31381 tataaccttc tgttgcctga aaattcttgg ccatattaat gaagttgttg ctaatctgtt
    31441 ctaaggcgct tttctctgat gcagattgtt tcgtgggcca gagctcttac gccttttaaa
    31501 gatcctttaa gtttcaaaat atctttatct tcctcaaaat gcagtttaca gttgtaggta
    31561 ttcccacttt ccgggtcata aatggtacct acccacttat ttttatcctt caaaaataca
    31621 atattatctg aaattactaa t
//
LOCUS       DAOSPF010000039        30897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1491_length_30897_cov_7.173270, whole genome shotgun sequence.
ACCESSION   DAOSPF010000039 DAOSPF010000000
VERSION     DAOSPF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30897
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1491_length_30897_cov_7.173270"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /locus_tag="PK252_08865"
     CDS             complement(<1..2666)
                     /locus_tag="PK252_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT14858.1"
                     /translation="MKKTLLLILGAITLFSCSPKQIENTENGAIIRLSQPMPNGAKLV
                     QISFETEKIVRVRATANMKFSKDSSLMAWDKPKVKVKVGFVQNGDTVTLTSSQVKVKV
                     LTTTGKIGFFDANGNEMLTESENGKSFTPIEVEGKKFWSIRQVFESPANEAFFGLGQH
                     QDGYMNYKGKDVDLFQYNTKVSVPFVISSRHYGILWDNYSQSKFGDSREYAQVSQFTL
                     YNKDGKIGGLTATYKSATDTSKIFVSQEEKEINYEFLPDLKKVPAMFPMGQGIVDWRG
                     YIASDTTGEHKFRFYSAGYAKVWVDGKLVIDRWRQCWNASALYFTLKMEKGKKYPVKI
                     EWNPDGGESYISLRALKPMAAGEQDKLSLYSEVAKQIDYYFIQGQTPDEIISGYRTLT
                     GAAPIMPKWALGFWQSRERYKTQDELLEVIEQYRKRNIPFDNIVLDWNYWPVDQWGSH
                     EFDTTRFPDAEGMIKKVHEQNAHLMISVWAKFYEGIENYKKLDEKGFLYKKNIEEGRK
                     DWIYPGYHSTFYDAMNPQARKMFWDMMSVKLFAKGIDAWWMDASEPDIHSNLPPADRK
                     AFMNPTALGPGAQYFNAFPLCNAKGIYEGQRSEKPDQRVFILTRSAYAGQQRYAAATW
                     SGDIGSRWDELQRQIPAGLNFCISGIPYWTTDIGGFAVEKRFEHAQGALLEEWREMMA
                     RWVQYGAFCPLFRIHGQFPYREIYNIAPENHPAYKAMVATDILRYRLMPYIYTLASKV
                     YFENYTIMRPMVMDFPTDTIVYTIANQFMFGPAFMVCPVTEYKARAREVYMPASTVWY
                     DWFTGKQIKGGQTIKAPAPYENIPIFVKAGSIVPVGSPVQYAMQKDENPVTLLVYAGA
                     NGKFSLYNDEGINYNYEKGQFENIDITYSDKEA"
     gene            3077..3664
                     /locus_tag="PK252_08870"
     CDS             3077..3664
                     /locus_tag="PK252_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT14859.1"
                     /translation="MIEDKDIVRTQIIEEAAKIFGHFGYKKTSMEQIALALNKGKSSI
                     YYYFTSKEAIFEAVIEKEVAFMRKKNHEAMAKASNVAEKFKAYIVSRMKGFEEMANMY
                     QVLKNEYLSHITFINQIRKRYDHQEIENIRLILEEGVGSGEFNISNTELAAVAIATAI
                     KGLEIPLFFSGEKVNVEHQLENLVHIVFNGILIRK"
     gene            3869..5353
                     /locus_tag="PK252_08875"
     CDS             3869..5353
                     /locus_tag="PK252_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT14860.1"
                     /translation="MKVFSYFLKKWLMGVLLLCSFNCLRAQDSTAKSLPSLVELAQRA
                     INKSEQLAIDSLVVRKAQLDKQKAWEAYLPKVTAEATYTRLNDDIVFPQNLQNLLLGT
                     QRLLIKEGAAMQTAALPDAYKVSFSTPYIVNTTNPNAPSNTLMSAVQQNLQEIPPIQE
                     KNILKANIQGQMLLFSGLKVPYLIKAANHQEKASRWMSESDKMKTINEVVNTYDKLAV
                     LDQTAIAIRQTRALLNEQQHYIEKAFKNGLTIDLNRQKIQLAIEQLSVREYEIESNKK
                     LLFIRLEELTGFLADSAALLHPELQIWQTDVLQGDAQNRPDIKAIDEAILATGYKQKS
                     EWAEYIPKVVAVGKKELITSDLSMLDPEWYVGVAVRWTIFDGLAAKTSAQQAKIDKLI
                     LERKRDDAVQLAQLNLKKTEYDFQKYLLNVSSTKKQVEIAQSILSLSKKQFEQGLITP
                     SEYMAAIVDLEKARLEYIQALAAQRAATASYLFASGNLTLDKIK"
     gene            5375..6352
                     /locus_tag="PK252_08880"
     CDS             5375..6352
                     /locus_tag="PK252_08880"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT14861.1"
                     /translation="MITNKVNKVNLVVLSLIGAVIITSCSSKNTNSLEGKVKRETICV
                     APKVPGRIAKIFVTESQIVKKGDTLAELYVPEIEAKMQQANGASQAATWQYNMAKHGA
                     TKDQKDQVIAMYEAAKEQLNLAEKSLARIKALYNDSLVSAQAYDEALTKYAMARSQCE
                     AAAAKKQEVVGGLRDEQVRMAQGQMNQAYGALREAQNAYNERFIVAPQDMTIETIALH
                     EGELALAGYSIFTGYSPSATYFRLTVPESKICDFKIDSIYTFCLPFMDNKEVKAQLTA
                     VNELAKYAFKSSAYPQYQMGEAVYELKFVPVSKSVNDKLFNNFTVVLKY"
     gene            6453..7574
                     /locus_tag="PK252_08885"
     CDS             6453..7574
                     /locus_tag="PK252_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14862.1"
                     /translation="MLLFFGAPILYALLTGYVYKSATIHDLPIVVVDLDNSPLSNKII
                     DALDDNTYLKVAEVNYEQKNVDDEIIRKNYVAVVTIPIGFEGNILQKRPAEIDADING
                     ANMLTANYASTGIQTVLSIINSGIEIEALQKKGIPNAIANRQFEGFRINITRFYNPAS
                     NYLIFLWPGLLGVILQQVFLLVLALSFAKEFEDNTFKNLLKYSQNSMYLIFLKSIPYW
                     LLGIALWLPITRLYFYWFNVPMVQHLGAFYLISALFILSLAFMGIASSIVFKTQLRAT
                     EVLMIIATPSFIISGQTWPLSEMPVIIRYISDIIPLTHFLEGFRKLLLCNASLSEIMP
                     QVVNLAILSVVFLVIAFFSLRYRITGKSYLIKKIKARFW"
     gene            7644..8237
                     /locus_tag="PK252_08890"
     CDS             7644..8237
                     /locus_tag="PK252_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOT14863.1"
                     /translation="MSKIGFILLTIFTNACLVYADNGHPQKDSFSEKTSHDTIFNADI
                     QPVYIYSPRTFKESEKNELLRYQRLVYNVKKAYPFAKIAAQKLREVNENLRYIKTKKE
                     KEAYIARTEDEMKKQFENDLKKLTITQGKILIKLFYRETGSTTYEVVKELRGSFEAFF
                     WQALARLFGSSLKTKYDPLGEDADIEMIVQLIESGQL"
     gene            8234..8803
                     /gene="thpR"
                     /locus_tag="PK252_08895"
     CDS             8234..8803
                     /gene="thpR"
                     /locus_tag="PK252_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT14864.1"
                     /translation="MKKRLFIALPLTIRGHVDINEWVTDKAINENEISWVKPENWHLT
                     LHFLGETNVSDIPALKRMIDEVCREFSVCEAEMKGMGSFANLKLPKVLWLGFNGVQSV
                     ISAQKIMADKLSGLGFVVETRLFKPHLTVGRIKRLGNVKALNSLLNKYKNSFFAKVQF
                     DRIVLFESILTPQGPVYKEIHTAYLTGHL"
     gene            complement(9005..9505)
                     /gene="tpx"
                     /locus_tag="PK252_08900"
     CDS             complement(9005..9505)
                     /gene="tpx"
                     /locus_tag="PK252_08900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOT14865.1"
                     /translation="MAKITFKGNPVNTAGSLPAVGSKAPDFELTKTDLGSITLAELKG
                     KKVVLNIFPSVDTGVCAASVRQFNAKAAGLSNTVVVCISKDLPFAHARFCGAEGINNV
                     VSASEYKDDNFSKSYGVLMTDGPLKGLLSRAVVVIDTDGTVKYTEQVPDIVQEPNYDG
                     ALDALK"
     gene            complement(9577..11631)
                     /gene="ppk1"
                     /locus_tag="PK252_08905"
     CDS             complement(9577..11631)
                     /gene="ppk1"
                     /locus_tag="PK252_08905"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOT14866.1"
                     /translation="MSGKKKIINRDLSWLSFNERVLQEAIDETVPLVERMRFLGIYSN
                     NLDEFFKVRVATLKRMIDYAYNPKELSGEKPAKILAQIQEKVLVQQQKFSQAFDNIIN
                     EFEKENIYFINEQQLNEEQAYFVDNYFDTNILPFLSPIMLSNVENFPEMKDKSIYFAI
                     KLSNSKRKVKTEYVLMEIPSILNRVVRLPSDGNRQCIILIDDIIRFCLKDLFAIFSFD
                     KFEAYTIKLTRDAELDIDNDLSKSLVEMISQGVSSRSHGQPVRFVYDQNIPNDLLKYI
                     INKLKFDNTDTIIPGARYHNFKDFITFPNIGEKRLEYPSTPAVEHPQIDKHASIIETI
                     MHKDVMLHYPYQKFDRFVDFLREASMDPKVKSVKITLYRLSKNSKVVNALINAARNGK
                     DVTAVIELQARFDEKSNIYWTRKLEEAGVNVMFGIKGLKIHAKLVLITRQEGKILKSY
                     AAVCTGNFHEGNAAVYTDVALLTSDKRITNEVARVFQYFDSPYWNYSYRHLLVSPLYM
                     RSGLIKLIDNEIKNAKAGKDAYIILKINNLVDSDMVKKLYQANNAGVRIKLIVRGICC
                     LTPGIEGLSENIDAISIVDKFLEHSRVFVFCNNNDEKYYISSADWMTRNLDHRVEVAC
                     PIYDEEIKKEIWMKLEVQLSDNTKARILNEAQDNQYVRNDAPPIRAQFVLYDYYKNKA
                     KS"
     gene            complement(11638..12144)
                     /locus_tag="PK252_08910"
     CDS             complement(11638..12144)
                     /locus_tag="PK252_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT14867.1"
                     /translation="MKEIKHLHILRHGKASWEYPELEDIDRPLLPKGVNNVMQVAELF
                     HEKYNPPDLIISSSAARAIHTALIMARLLNVSPHKVRIDERLYETDSSTVLEVVESVE
                     AKISNLLIVGHNPIFTRFVNLFLPEYLENLPTSGLVTLTFKIKDWSIINSIPIKSDIT
                     LPPSKRLI"
     gene            complement(12281..13096)
                     /locus_tag="PK252_08915"
     CDS             complement(12281..13096)
                     /locus_tag="PK252_08915"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOT14868.1"
                     /translation="MASKTPINIELQKGKKAYFCSDMHLGLYPYDKSAQREKMIVLWL
                     EVIRKDAQILFLQGDIFDFWYEYRKVAPRGFVRFLGKLAELADSGIEIHYFTGNHDVW
                     VFDYLPKEIGLQVHYTDRLFEISGKRFLIGHGDELCKTDYGYRFLKGCFKNKFLQFLF
                     SRLHPNFALAIGHTWSKHSRLSKGVSEAFLGEDKEHQIQFAKQYLNTNEIDFFVFGHR
                     HIPLDYKLNDKSRLINLGEWIVSNTFATYDGENMMLKKYENDIFIEYTRNLGE"
     gene            complement(13086..14240)
                     /locus_tag="PK252_08920"
     CDS             complement(13086..14240)
                     /locus_tag="PK252_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOT14869.1"
                     /translation="MIKKYIGKACVIFLSAIICFTSCTKEKYRVNGVLERWKNTKIYW
                     TEYNITSEKLIDSAQTDQNGKFVLKGELQAPGIFKLYVARDNYLLLAIAPGEKIDLNC
                     PSSKIYENYQVEGSAYSKEAKLLQDQLNNTVKAIDSISKTNQSKDSMNIAIKKIYDQQ
                     HSFSVKFVLEHLRSPSSIICLFQQTDSQTYVMGKIRDMQYLKLASDTLKKYFPESKPV
                     KALWVERNRIFEQLKNAKSQGKYLEAEVRTFPEIKLPSKDGDTIKLSQVVEKSKLTLL
                     LFWTPTNSDCVVVMKSINNLIERYGNKGLEVYNVALFNDIGYWSNFIDKAQLKGYQVI
                     DENVGNSVYSSIYNVQFIPSIYLINKEKGVLTINVFGEKLETEIKKYLGK"
     gene            complement(14259..15428)
                     /locus_tag="PK252_08925"
     CDS             complement(14259..15428)
                     /locus_tag="PK252_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT14870.1"
                     /translation="MKKVNFLLGILTIGLFVTSCSNEESFKVKGKLRNASGEMLFLKE
                     LTISDIKTIDSVIVDNDGSFVLKGINNKIAFYTLGLDKKNTLTLVIKPGDKIFVQADA
                     KDLPNEYDVKGSEESQLVKKLTIKLNETLEKINQLGKTFHDSIGSKNLLKIKSELDSV
                     YKIIESDQKSFSINFIRENKGTLASVMALYQQMTPRKSLFTPANDYAIFKEVDSVMMQ
                     TYPEADAVQSLHSLVGNISEEYIRKVQIEQRLTKGAPIPEINLPDFNNQPLALSSLRG
                     KYVLVLFWASSNQKSIEASKEFIGQYWRYKNVGFDVYHVCIDKSRDAWLKAITVNGFP
                     WHNVSDLKMWDSPVVALFGIEELPFNLFIDKEGKIVEKQITSQKLAERLKEIFKY"
     gene            15782..17728
                     /locus_tag="PK252_08930"
     CDS             15782..17728
                     /locus_tag="PK252_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOT14871.1"
                     /translation="MSYLKFDKTQLVNLEYSLTKEIVRSNRAGSYSHTTIVNCNTRKY
                     HGLLVCPIEEIDGEKHVMLSSLDLTVIQHDAEFNLAIHKYPGDVYYPTGHKYIRDFEI
                     GKVALTRYRVGGVVITRESILVEKEEQLILKYTLEEAHSPTTLRFKPFLAFRNIHSLS
                     KANMYANTRVENVANGIKSRLYNGYPALYIQFSKKVEFIPAPDWNYNIEYPEEQARGY
                     DYSEDLFVPGYFEMHIKKGESIYISASLHEAQPASLSKIYAKEVNSREERNSFHSCLK
                     NAAKQFFVKKDKKTEITAGFPWFGTWGRDTFISLPGLTLFTDDEKIFKSVVDTMVDRM
                     KNGLFPNMGSDSNPAFNSVDAPMWFFWALQQYVNRHNSAAEIWERYGKTMKTILEAYR
                     TGISYNIRMHENGLIWAGEHGHALTWMDAVVAGKPVTPRMGYDVEINALWYNAICFCL
                     DLAKLGKDTRFINTYKHLPELIEKSFLETFWNEEKGYLADYVDNEHTCWQVRPNQIIA
                     ASIDYNLIPDDIKQKVIEVVKNELLTPYGLRTLSPNDEHYEPVYEGDQPARDKAYHNG
                     TVWPWLLQPFCIAYIKLYKKSGVPLLKRIYEGFEPILTEYGVGTIGEIFDGNPPHKPR
                     GAVSQAWSVAALITIEQLINDYDK"
     gene            17798..19090
                     /locus_tag="PK252_08935"
     CDS             17798..19090
                     /locus_tag="PK252_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT14872.1"
                     /translation="MKVLMFGWEFPPHISGGLGTACYGLTKGLSRHNDLEVLFVVPKA
                     FGDEDKSAAHIIGASDVYINQKKINIKGFLKEVNMIEVNSTLIPYTDLDEYYNIIQKY
                     NLHNNKNVVKSDFEGRFNFSGGYGPMLFNEIYNYSLVASMLASEHSFDIIHAHDWLTY
                     PAGIVAKEISGKPLVIHVHATDFDRSGGNVNPRVFEIEKRGMEVADKIITVSNWTRDI
                     VINKYGINPNKVVTVYNAVEPLNEAETSRYEKYLDDKIVTFLGRITMQKGPEYFIEAA
                     RKVLNRMDNVRFVMAGSGDMMNKMVLRVAQLGIANRFHFTGFLAGDDVNNMYALSDVY
                     VMPSVSEPFGISPLEAIRSNVPVIISKQSGVAEVLNHAIKVDFWDIDALADAIYGILN
                     YPALSKMFKTYGKEEVDNLKWENSADHVRAIYYQTISA"
     gene            19119..20528
                     /locus_tag="PK252_08940"
     CDS             19119..20528
                     /locus_tag="PK252_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOT14873.1"
                     /translation="MKTICLYFQVHQPFRFRRYRFFEMGKEHYYYDDYSNESIMRKVA
                     MKSYLPANKILLEIFKEYGCRFKVAFSISGTALDQMELYAPDVLESFQKLAASNCVEF
                     LSETYSHTLASLINREEFENQVKEHQERILKLFGKKPVVFRNTELIYNNEIGEWIADM
                     GYKAAIIEGAKHILGWKSPNYVYCSAVNPRLKLLLKNYMLSDDLTFRFSDVHWNQYPL
                     TAEKFTSWIKHMDQKEDTLNLFMNYDTFGNIQPKESGIFEFLKAFPKEVLSTNTLSFS
                     TPSDLIEKFQPISALSVPHAISWADEERDTSAWLGNELQKEAFYKLFNLRDMVLQTND
                     PKILTDWKYFQTSDHFYYMCTKFFVKGSMHSYFNPFSSPYDAFINYMNILSDFEIKIK
                     LALSSNNSGIKKTNGNGTKKTNNIAAKTDKEKVKKVVKKTTAKTAKPTKEDVVVEKTN
                     KKTAKKETTKEKVKKSKKA"
     gene            20549..24799
                     /gene="glgP"
                     /locus_tag="PK252_08945"
     CDS             20549..24799
                     /gene="glgP"
                     /locus_tag="PK252_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOT14874.1"
                     /translation="MSNKTTIMPDYLFEVSWEVCNKVGGIYTVVSTKALTLSQQLGSN
                     YILIGPDVWRDGVVNPEFVEDKRLFKSWRDKAAEEGLRIKVGHWNIAGNPVAIIVDFT
                     TFISQKDTIFSKFWEKYKLDSITGQWDYIEPCLFGYAAGRVIESFAKFNLSVRDRIVA
                     QFHEWMTGSGLLYLKSEMPQIGTVFTTHATAMGRSIAGNNQPLYSNIKDYIPDLKAAE
                     FNIISKQSLEKNSAQHADCFTTVSEITNLECEAFLGKNVDVVTPNGFEDSFVPTADKF
                     DQARLQARKKLIEVASALTDSKINDDVLLIANSGRYEFRNKGIDLFIDSLGEINKSGH
                     LNKEVVAFILIPANHYGPRRDLANKLKGQEYEHCDECILTHNLHYPEHDSILKRIKAN
                     GLTPSETNKVKVIFVPSYLNGDDGIFNMSYYQLLIGFDLTVFPSYYEPWGYTPLESIA
                     FKVPTITTSLAGFGLWIKQLSMSSENAVTVIERTDINDAEVTHQIAGQILKFSNLNAE
                     QVENARNLSFEMSRTALWKNLVSYYLKAYEIALSKVQERTSTIAETEKIETLTAAEKF
                     SVTNDPVWKRLLVQKNIPEELHALEELSKNLWWSWNYDAIDLFRSIDPELWVKSDENP
                     IVLLDLIPYEKILKLSKDVVFLKKLNDIYNKFLEYQNKPCKVGPKIAYFSMEYGLHDS
                     IKLYSGGLGVLAGDYLKEASDANIDIIGVGLLYRYGYFKQVISEHGDQIAQYESQKFS
                     QTPVQPVRDANGQWLTIHINLPGRPLIARIWRLSVGRIPLFLLDTDFEDNIEQDRSIT
                     HHLYGGDWDNRFKQELLLGVGGIRALDTLGISPDLFHCNEGHAAFIGVERLRKYIVDQ
                     NLTFAEGLELVRSSQLFTTHTPVPAGHDAFDESMMRTYMGHYPKRLKISWLQFMALGK
                     AHPDNPSEKFSMSVLAANLSQEINGVSELHGRVSQEIFHDMYRGYAAEELNIGFVTNG
                     VHMPTWTARPWMELYKSTFGDDFFEKQTKFETWAKIQQVPNSNIWKIRNSQRQILVDY
                     IKDRLKDAAIKRLENPKMVVEMCDRLNKHNLTIGFARRFATYKRAHLLFNDIERLSRI
                     VNNPSMPVQFVFAGKAHPHDKAGQDLIKLISEISKKPQFVGKIIFIPNYDMKLAKILV
                     QGVDIWLNTPTRPLEASGTSGEKAVMNGVLHFSVLDGWWAEGYVDGAGWALPEEVTYQ
                     NNAYQDQLDAETIYNLIENEIAPLFYFRDENGLPNGWVQYIKNSIEKVASHFTMSRQL
                     EDYNTKFYTKLYDRTLKVQANDYQLAKEMARWKKKVRSSWESIEVVSTNIPDVSREAI
                     SLGNSYVAQIELDLNELAPEDIGVELVLADLVGDESKPKVSYCQEFELVKYDGQIAQY
                     RIEMRPQKTGVYEYGIRLFPKHPELPHRQDFNYVRWLE"
     gene            complement(25126..25479)
                     /gene="rplS"
                     /locus_tag="PK252_08950"
     CDS             complement(25126..25479)
                     /gene="rplS"
                     /locus_tag="PK252_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT14875.1"
                     /translation="MDLMNIVKENLAAKTEFPAFKSGDTITVHYKIKEGNKERIQQYK
                     GVVIQKKGTGHTATFTVRKMSGNIGVERIFPIASAFIDKIELNKVGKVRRARIYYLRD
                     LTGKKARIQEKRAIQ"
     gene            complement(25569..26087)
                     /locus_tag="PK252_08955"
     CDS             complement(25569..26087)
                     /locus_tag="PK252_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14876.1"
                     /translation="MNITEWIIIISFFIIVSGTNIYLVWLFLRNAEKNRKVELLLKTH
                     EMMMPLRLQAYERLILFLERISIDMLLTRHSRSGITSRQLHTELLSSIRKEFEHNISQ
                     QLYVSAKAWEMIKNARAEIVKIINLAAESTDPKAPGIDLSKKILEDISNIDKLPLQKA
                     IDYIKQEAKEIF"
     gene            complement(26100..26711)
                     /locus_tag="PK252_08960"
     CDS             complement(26100..26711)
                     /locus_tag="PK252_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT14877.1"
                     /translation="MINEIKQILEHEAKSILNIPIDSCYEKAIDLIYERVHVKRGKLV
                     ASGMGKAGQIALNMATTFSSTGTPAVFLHPSEAQHGDLGIIQENDILILISNSGKTRE
                     IIELTHLARRLQPGIPIILITGNPNGELSSEAEVTLSTGNPKEVCILGLTPTTSTTAM
                     TVIGDVLVVQLMKKINFTAEDYAKRHHGGYLGVLSRKQSTEDK"
     gene            26843..28645
                     /gene="argS"
                     /locus_tag="PK252_08965"
     CDS             26843..28645
                     /gene="argS"
                     /locus_tag="PK252_08965"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT14878.1"
                     /translation="MEKIESNLVASVIKAVGSLYNKSIEPNFVQIQKTRKDFKGDITI
                     VVFPLLKFSGKGPEQTANDLGNYLVQHFELVEGFNVIKGFLNIEISKQYWFSFLEDIY
                     LTKGLEGGQLFHTSSPKKIMVEYSSPNTNKPLHLGHIRNNLLGYAVANILKATGNEVL
                     KVNLINDRGIHICKSMLAWQKFGNGATPESTGIKGDHLVGDYYVRFDQEYKAQTKELM
                     SQGMTEEEAKKQAPILLEAQEMLRKWEAGEEEVLSVWRMMNQWVYNGFDETYKRLGVD
                     FDRLYYESQTYLLGKQIVLDALTKGLLYKKEDGSVWADLTEKGLDHKVLLRSDGTSVY
                     MTQDIGTAHQRFEQYHIDSHIYVVGNEQNYHFQALAIVLGKMGFDWADKIFHLSYGMV
                     ELPEGKMKSREGTVVDADDLMDEMISTARQMSEELGKLEGLSAEDINKTLNIIGLGAL
                     KYFILKVDPKKNMVFDPKESIDFNGNTGPFIQYTYARIKSILRKAADAQIVFDLKQAS
                     AVNMGDKEKELVKLLYDFPMVVQEAAKEFSPAIIANYCYDLAKEYNQFYHDCPILKEA
                     DIAVKSLRFALSQQVSQAIGYGMKLLGIDVPERM"
     gene            28801..28992
                     /gene="rpsU"
                     /locus_tag="PK252_08970"
     CDS             28801..28992
                     /gene="rpsU"
                     /locus_tag="PK252_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT14879.1"
                     /translation="MIIVPIKEGENLERALKKFKRKFEKTGTVKELRERQFFTKPSVE
                     KRQEIIRAIYRQKMQALDE"
     gene            29029..29901
                     /locus_tag="PK252_08975"
     CDS             29029..29901
                     /locus_tag="PK252_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT14880.1"
                     /translation="MLYIDEYLNYLKFEKRYSSHTVLSYNNDLQQFSLFIDCENPENI
                     KQQDVRQWIISLYDAGISPRSINRKLTALKAFFKFLLKNGKVAKNPVIGIKALKTKKS
                     LPQFVEESKIGSLLEDNYFEVGFEGYRNKIIIEMFYYTGIRLSELINLKTSDVDFFKQ
                     CIKVLGKRNKERIIPLTPKFVSHLNEYFKMKSESGVADSGFVFCSSKGKQLNPRTIYQ
                     VVNTYLKMVTSIEKKSPHVLRHTFATHMLNNGADLNAIKELLGHSSLAATEVYTHNTF
                     EKLTNIYKQAHPRA"
     gene            29919..30218
                     /gene="raiA"
                     /locus_tag="PK252_08980"
     CDS             29919..30218
                     /gene="raiA"
                     /locus_tag="PK252_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT14881.1"
                     /translation="MKIKLQSLHFDADQKLIDFIEKKADKLAKLYENIVGIEVILKLD
                     NDSKDENKVVEMKVEIPGNDLFSKKQCRTFEEAADQCVDALKTQLVKFKEKQRGE"
     gene            30367..30440
                     /locus_tag="PK252_08985"
     tRNA            30367..30440
                     /locus_tag="PK252_08985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30401..30403,aa:Thr,seq:tgt)
     gene            30456..30538
                     /locus_tag="PK252_08990"
     tRNA            30456..30538
                     /locus_tag="PK252_08990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30490..30492,aa:Tyr,seq:gta)
     gene            30620..30692
                     /locus_tag="PK252_08995"
     tRNA            30620..30692
                     /locus_tag="PK252_08995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30653..30655,aa:Gly,seq:tcc)
BASE COUNT         9414 a         5851 c         5746 g         9886 t
ORIGIN      
        1 gcttctttgt ctgagtatgt aatatcaatg ttttcaaatt gtcctttttc gtagttgtag
       61 tttatgcctt cgtcgttgta aaggctgaac ttcccgttgg caccggcata tacgagcaat
      121 gtaaccgggt tttcatcttt ctgcatggcg tattgaacag gcgatcctac tggtacaata
      181 gagccggctt tgacaaatat cgggatgttt tcataagggg caggggcttt gatggtttgc
      241 ccccctttga tttgtttgcc tgtaaaccag tcgtaccaaa ctgtactggc tggcatatat
      301 acttctcttg cacgggcttt atattctgta acagggcata ccataaaggc cgggccaaac
      361 ataaactggt tggcaatggt ataaacaata gtatcagttg gaaagtccat caccattggg
      421 cgcatgatgg tataattttc aaaatacacc ttactggcaa gggtatatat ataaggcatc
      481 aggcgataac gcaaaatatc tgtagctacc atagctttgt aagccggatg gttttcaggt
      541 gctatgttgt atatttcgcg atatgggaat tgtccgtgaa tcctgaacaa tgggcagaag
      601 gctccgtact gtacccaacg ggccatcatc tcgcgccatt cttcaaggag tgcgccttgt
      661 gcatgttcaa agcgtttttc aacagcaaat ccacctatat ctgttgtcca ataagggata
      721 cccgaaatgc aaaagttcaa tccggcaggt atctgacgtt gtaactcgtc ccagcgtgaa
      781 cctatgtcac cgctccaggt tgctgctgca tacctttgct gtccggcata agctgagcga
      841 gtgaggataa ataccctttg atctggcttt tctgagcgtt gtccttcgta tatccctttg
      901 gcattacaaa gagggaaggc attgaaatat tgagcaccgg ggcctaatgc agttgggttc
      961 ataaatgctt tacggtctgc tggtggcagg tttgaatgta tgtctggttc ggatgcatcc
     1021 atccaccagg catcaatacc tttggcaaaa agctttacac tcatcatatc ccagaacatt
     1081 ttacgggctt gagggttcat agcatcgtaa aaggtgctgt gatagcctgg ataaatccaa
     1141 tctttgcgtc cttcttctat atttttcttg tataagaagc ctttctcatc cagtttttta
     1201 tagttttcaa taccttcata aaactttgcc cacacagata tcatcaaatg tgcattttgc
     1261 tcgtgtactt tttttatcat cccttcagca tcggggaaac gggtagtatc aaactcgtga
     1321 ctgccccatt ggtcaacagg ccaatagttc cagtcgagta ctatattgtc aaatgggatg
     1381 ttgcgttttc tgtattgttc aattacttca agcagctcgt cttgcgtttt gtaacgttcg
     1441 cggctctgcc aaaatcctaa tgcccactta ggcataatag gtgcagcacc tgtcaatgtt
     1501 cggtatcccg aaattatttc atcaggtgtt tgcccttgta tgaaatagta atctatctgt
     1561 ttggcaactt ctgaatataa ggatagttta tcttgttcgc ctgccgccat aggcttcaat
     1621 gcccttagac tgatgtatga ttcgccaccg tcagggttcc actcaatttt aacagggtac
     1681 tttttgcctt tctccatttt tagggtgaaa tataaggctg aggcattcca acattgtctc
     1741 cagcggtcaa ttaccagctt accatcaacc cagaccttgg catatccggc agagtaaaat
     1801 cgaaacttat gctcacctgt agtatcagat gctatataac ctctccaatc aacaatacct
     1861 tgtcccatgg ggaacatggc aggtaccttt ttcaaatctg gcagaaattc gtagttgatt
     1921 tctttttctt cttgtgatac aaatattttg ctggtgtcag tggctgattt gtaagtggct
     1981 gtcagccctc caatttttcc atctttatta tataacgtaa attgcgaaac ctgggcatac
     2041 tcgcgactat caccaaactt gctttgcgaa tagttgtccc acaatatgcc atagtggcgt
     2101 gacgaaatta caaatggcac tgacaccttt gtattatatt gaaaaagatc aacatctttt
     2161 cctttgtagt tcatgtagcc atcctgatgc tggcctaacc caaagaaggc ttcattggcc
     2221 ggcgactcaa atacttggcg gatgctccaa aacttttttc cttcaacctc tataggggta
     2281 aaacttttcc cattttctga ttctgtaagc atttcgttgc cattggcatc aaaaaaacca
     2341 attttaccgg tagtggtaag tacctttact ttaacttgtg aactggtaag ggttaccgtg
     2401 tcgccgttct ggacaaaacc aacttttact tttactttgg gcttatccca agccataagg
     2461 cttgagtctt ttgaaaactt catgttggca gtagccctta ccctgactat tttttctgtt
     2521 tcaaaactga tttgcaccaa ttttgcccca ttgggcatag gctgcgaaag ccttataata
     2581 gccccgtttt cagtgttttc aatttgtttt gggctgcatg aaaataatgt tatagctccc
     2641 aatatcaaca ataaagtctt tttcatccgt taataattta atttttttaa tatggtcgta
     2701 tggaactcgt cccggttagt cggggttcat ccttgtttgt gtttaacgaa tcatgtttgt
     2761 ttcattttta gttttagcag aagcttaaca cacatatgat agcatacaaa tgtattttaa
     2821 gctttaggct gtttgtatat aatggtgttt ttaaactata tacgagatgt taaatcgtgg
     2881 tatctaaatg ttaacaatag ggggcagttt tgataatatt tctttttgtc caccttcaat
     2941 aaaattaccc acataagact tctgccatca aagaccagac ttgttattgt aaattttttt
     3001 gaaaaaaaat gctgacaata ttattttggc attgtttttg gaacagaata gtcgaacggt
     3061 cgaaaaatat atttttatga ttgaagataa agacattgtt agaacccaaa taattgaaga
     3121 agcagctaaa atatttggac attttggtta caaaaaaacc agtatggaac aaattgcctt
     3181 agctttaaat aaagggaaga gctctattta ttactatttt acaagtaaag aggccatatt
     3241 tgaggctgtt atcgaaaaag aggtggcctt tatgcgaaag aaaaaccatg aagctatggc
     3301 caaagcttcc aatgttgctg agaaattcaa ggcatacata gtatctcgta tgaaaggatt
     3361 tgaagaaatg gctaatatgt accaggtgtt gaaaaatgaa tatttgtcgc atattacttt
     3421 tatcaaccaa attcgcaaaa gatatgacca tcaggagatt gaaaacattc gtttgatatt
     3481 ggaagagggt gttggcagtg gagagtttaa tataagtaac acagaactgg ctgctgttgc
     3541 tattgctact gcaattaaag gccttgaaat ccctttgttt ttttcaggag agaaggtcaa
     3601 tgttgagcac cagttagaaa acctggtaca tatcgtcttc aatggcatac ttatacgtaa
     3661 ataaaaaatt tttttgaaat aatttcgacc aaatgaacaa aatagtcgaa caataaaaat
     3721 ggtcaataaa atgaaacact catgattgtg gccaaacaaa agcactcatg aatcccgaca
     3781 aatcatgacg ggtgttctat ctgcttatta tatgacaaat attgatagtt taaatgatta
     3841 aaattcaata aattaaaagt tcatacgaat gaaagtgttt agttattttt tgaaaaaatg
     3901 gttgatgggg gtgctgcttt tatgtagttt caactgcctg agggcgcagg acagcaccgc
     3961 aaaatcgttg ccatcgctgg tagagctggc tcagcgtgca atcaacaaaa gcgaacaact
     4021 tgctattgat agcttggttg ttcgtaaggc gcaactggac aaacaaaagg cttgggaggc
     4081 ctatctgccc aaggtgacag ctgaagccac ttataccagg ctcaacgatg acattgtttt
     4141 tccgcaaaat ttgcaaaacc tgcttttggg gactcaacgt ttgctgatta aagaaggcgc
     4201 tgccatgcaa actgctgcat tgcccgatgc ttataaagtt tcattctcta caccttatat
     4261 tgtaaatact acaaatccca atgcgcccag taatacctta atgagtgctg ttcagcaaaa
     4321 tcttcaggag attcccccta ttcaggagaa aaatatccta aaagccaaca ttcagggaca
     4381 aatgttactc tttagcggcc tgaaagtacc ttatcttatc aaagctgcaa accatcagga
     4441 aaaagcatcg aggtggatga gcgaaagtga caagatgaaa accataaatg aagtggtaaa
     4501 tacttacgat aaacttgctg ttctggatca aactgccata gccattcggc aaactcgggc
     4561 tttactcaac gaacaacaac attatattga aaaagctttt aaaaacgggc ttaccattga
     4621 cctcaaccgt caaaaaatac aacttgccat agaacagctc agtgtcagag aatatgagat
     4681 tgagtcgaac aaaaaacttt tatttatcag gttggaagaa ttgacaggct ttttggctga
     4741 ttctgctgct ttgttgcacc ctgagctgca aatatggcaa actgatgttt tacagggcga
     4801 tgcccaaaac aggcctgata taaaagcaat agatgaagca atccttgcta ctggttacaa
     4861 acaaaagagc gaatgggctg aatatatacc aaaagtcgtt gctgtaggta aaaaggaatt
     4921 gataacatct gatttgtcta tgctcgaccc tgaatggtat gttggagtgg ctgtacgatg
     4981 gactattttt gatggtttag ctgccaaaac atctgcccaa caggcaaaaa ttgataagtt
     5041 gattcttgaa cgcaaacgtg atgatgctgt tcagttggcc cagcttaatc taaaaaagac
     5101 agagtatgat tttcaaaaat atttgttaaa cgtatcttct actaaaaaac aggttgagat
     5161 agcccaaagt attctatccc tcagcaaaaa gcagtttgaa caaggcctta ttactccatc
     5221 tgaatacatg gctgctattg ttgatttgga gaaagctcgt ttggaatata ttcaggcttt
     5281 agctgcgcaa cgtgctgcta ctgccagcta tttatttgct tctggcaatt tgaccttaga
     5341 taaaattaag taagaacatt aaaaacatca tatcatgatt acaaataaag taaacaaagt
     5401 aaatttggta gttctctcat taataggagc cgtaattatt acatcttgca gcagcaagaa
     5461 taccaatagc ctcgaaggta aagtaaaacg cgaaacaata tgtgttgcac caaaagtccc
     5521 cgggcgtatt gcaaagattt ttgtcacaga atcacaaatt gtcaagaaag gtgacactct
     5581 tgctgagctg tatgtccccg aaattgaggc caaaatgcaa caagccaatg gtgcaagcca
     5641 ggctgctact tggcaataca atatggcaaa acatggcgct actaaagacc aaaaagatca
     5701 agttattgct atgtatgaag ctgcaaaaga gcaattaaac ctggctgaaa aatcccttgc
     5761 ccgtatcaaa gccttgtata acgatagcct tgtttctgct caggcgtacg atgaagcatt
     5821 gaccaaatat gcaatggctc gttcgcaatg cgaagctgct gctgctaaaa aacaagaagt
     5881 agttggtggt ttacgcgacg aacaggtccg aatggcccaa gggcaaatga accaggcata
     5941 cggcgcttta cgtgaagctc aaaatgcata taacgagcgt ttcattgtgg ccccgcaaga
     6001 tatgacaata gaaacaatag ctttgcatga gggagaactt gctctcgccg gatattctat
     6061 ttttactgga tatagcccct ctgctactta ctttcgtctt actgttcctg aaagtaaaat
     6121 ttgcgatttt aaaattgaca gcatttacac gttttgtctg cctttcatgg ataataagga
     6181 agtgaaagcc caattgacag ctgttaatga attggccaaa tatgctttta agtcaagcgc
     6241 ttatcctcaa tatcagatgg gtgaggctgt gtatgagctt aaatttgtac ctgtaagtaa
     6301 gtctgtcaat gataaactct tcaacaactt cacggtggta ttgaaatatt agtaccttat
     6361 tttatttagg actttaatat tataacataa tgaaactatt tagtaagtta ataaaacgag
     6421 agttcaaact tttttttaaa aatgagtttg ccttgttatt attctttgga gcgcctattt
     6481 tatatgccct tcttaccggg tatgtgtata aaagcgctac tatacacgat ttgccaattg
     6541 ttgttgtcga tctcgataat tcgccactaa gcaataaaat tattgatgct cttgatgata
     6601 atacttattt gaaggttgct gaggttaatt atgaacaaaa aaatgttgat gacgaaatta
     6661 ttcgcaaaaa ctatgttgct gttgttacca ttcctattgg ttttgaagga aatatacttc
     6721 agaaaaggcc agccgaaatt gatgctgata tcaatggggc caatatgtta actgccaatt
     6781 acgccagtac tggtattcaa acagtgctat ctattataaa ctcgggtatt gaaattgaag
     6841 ctcttcagaa aaaaggcatc cctaatgcta ttgcaaaccg acaatttgaa ggttttcgca
     6901 tcaatattac tcgtttctat aatcctgctt caaattacct catttttttg tggccaggcc
     6961 tgttgggtgt tatcctgcaa caagtgttct tactggtatt ggccttgagc tttgctaaag
     7021 aatttgaaga caataccttc aaaaaccttc tgaaatatag ccaaaatagc atgtatctta
     7081 ttttcttaaa atcaatacct tattggttat tgggaatagc attatggttg ccaattacaa
     7141 ggctttattt ctattggttc aatgttccta tggtccaaca tctcggtgca ttctatctta
     7201 tatcggcttt gtttattttg tcgttagcat ttatgggtat tgcatctagc attgttttta
     7261 aaacccagct tagggctact gaagtgctaa tgattattgc tactccaagt tttattatta
     7321 gtggtcaaac ttggccgttg tctgaaatgc ctgttattat tagatatatt tcagatataa
     7381 ttcctcttac ccattttctc gaaggcttta gaaaattatt gctttgtaat gcttcattat
     7441 ccgagattat gccgcaagtt gttaatttgg ctatcctctc tgttgttttt cttgtcattg
     7501 ctttttttag ccttagatat cgcattactg gcaaaagtta tttgattaaa aaaatcaaag
     7561 cccgtttctg gtaatatttc cttcatacaa tgagcggata tggctgtata attgtttaat
     7621 tttgcaacta tatccgcttt ttcatgtcta aaataggttt catattatta actattttta
     7681 ctaacgcgtg tttagtatat gcagataatg gacatcccca aaaagatagc ttttcagaaa
     7741 agacttctca tgatactatt tttaatgctg acattcagcc tgtttatata tattcacccc
     7801 gcacttttaa agaatctgaa aaaaatgaat tacttcgata tcagcgctta gtgtataatg
     7861 taaaaaaagc atatcctttt gccaaaatag ctgctcaaaa gctgagggag gtaaatgaaa
     7921 accttagata tatcaaaaca aaaaaagaaa aggaagctta tattgcccgt actgaggacg
     7981 agatgaaaaa acaatttgaa aatgacctta aaaaattaac tattacacaa ggtaaaattt
     8041 tgattaagct cttttatcgc gaaaccggaa gtacaactta tgaagtagtc aaagaattgc
     8101 gtggtagttt tgaagctttt ttttggcaag ccctggcacg gctctttggt tcaagcctga
     8161 aaactaaata cgaccctctt ggcgaagatg ctgatattga aatgattgtt caactaattg
     8221 aatccgggca gttatgaaaa agcggctgtt tattgccctt cccctgacta ttaggggaca
     8281 tgttgatata aacgaatggg ttacagataa agctattaac gaaaacgaaa taagctgggt
     8341 aaaacccgaa aactggcatc ttactctcca tttccttggt gaaacaaatg tttcagatat
     8401 acctgcctta aaacgtatga tagatgaggt gtgtagagaa ttttcagttt gcgaggctga
     8461 gatgaagggt atgggctctt ttgccaacct aaaattgccg aaggtattgt ggcttggttt
     8521 caatggcgtt caatcggtta tatctgccca aaaaattatg gccgataagc tctctggtct
     8581 tggctttgtt gtcgaaacaa ggcttttcaa accccatctt actgttggcc gcataaaaag
     8641 gcttggcaac gttaaagccc ttaacagtct cctcaataaa tataaaaact ccttttttgc
     8701 aaaagtccaa tttgaccgta ttgttttgtt tgaaagtata ttaaccccac agggccctgt
     8761 ttataaagag atacatactg cgtatttgac gggccatttg taatttcggt tgcttaggca
     8821 atggcgttta gttaatcccg ctttagaaat tgtttgaaaa attttattaa attattgaat
     8881 tacaatttct taatcggtta aactttttat tgcttatttt ttgagataaa aaaaactacc
     8941 aaagctgtat taaacaacat tgatagttta ttgtagtatc ttctttcttc ttaaaggcta
     9001 aattttattt caaagcatcc agggctccat cataattcgg ctcctgaaca atgtcaggta
     9061 cttgttcggt gtattttacc gtaccatcag tatcaataac taccacagcc cttgaaagta
     9121 accctttcag cgggccatct gtcattagca ctccatagct ttttgaaaaa ttgtcatctt
     9181 tgtattctga tgctgatact acgttgttaa tcccttctgc cccacaaaag cgtgcatggg
     9241 caaatggcaa gtctttcgaa atacacacta caacagtgtt gctcaatccg gctgcttttg
     9301 cattgaattg cctcacagag gctgcacaaa caccagtatc aacactggga aatatgttta
     9361 gcactacttt cttgcctttt aactctgcta atgtgatact gcccaggtct gttttggtca
     9421 attcaaaatc aggagcctta gacccaactg ctggtaatga acctgccgtg tttacagggt
     9481 ttcctttaaa tgtaattttt gccatattct tcttttatta gttttattaa tcaaaccaat
     9541 aacttgaagt attgtttaat gtttctcaaa aaatatttaa ctttttgctt tatttttata
     9601 ataatcatac aaaacaaact gcgccctgat gggaggtgca tcgtttctga catattggtt
     9661 atcttgggct tcattcaaaa tacgggcttt ggtgttgtct gaaagctgaa cctccagttt
     9721 catccaaatt tcctttttta tttcctcgtc ataaattgga caggctactt ctactcgatg
     9781 atcgaggtta cgggtcatcc agtcagctga agatatgtag tatttctcgt cattattatt
     9841 gcaaaataca aatactcgcg aatgttctaa aaatttatcc acaatgctta ttgcatctat
     9901 gttttcgctc aatccttcta tcccgggggt caaacagcat attcccctta ctatcagttt
     9961 tatcctcacc ccggcgttgt tggcctggta aagtttcttg accatgtctg aatcaacaag
    10021 attgttaatt ttcaaaatga tataggcatc tttcccggct tttgcatttt ttatttcgtt
    10081 gtcgataagc tttatcaatc cactacgcat atataacggg gaaactaaaa ggtgcctata
    10141 gctgtagttc caataaggag aatcaaagta ttgaaataca cgtgccactt cattggtaat
    10201 gcgtttgtct gaagttaaaa gggcaacatc tgtatataca gcggcgttgc cttcatgaaa
    10261 gttcccggta cacactgctg catagctttt aagtattttt ccttcctgac gggtaataag
    10321 taccagcttt gcatgaatct tcagcccctt aatcccaaac attacgttta ctccggcttc
    10381 ttcaagtttt ctggtccagt aaatatttga tttctcatca aaacgagctt gtagctcaat
    10441 cactgctgtt acatctttac cattacgtgc tgcatttatt aatgcattga ccacttttga
    10501 attttttgaa agcctgtata gtgtaatttt tactgattta actttagggt ccatcgatgc
    10561 ttcccgcaaa aaatcaacaa aacggtcaaa cttctggtag gggtaatgca acatcacatc
    10621 cttatgcatg atagtttcaa taatactggc atgcttgtct atctgtggat gctcaaccgc
    10681 aggtgttgaa gggtactcaa gtcgtttttc gccaatattc gggaatgtta tgaaatcttt
    10741 gaaattgtga taacgtgccc ctgggataat ggtatctgta ttgtcaaatt ttagcttgtt
    10801 gataatatat ttgagcaggt cgttaggtat gttttggtca tatacaaacc tgacaggctg
    10861 tccgtgacta cggctgctta ccccttgcga aatcatttct acaaggcttt tagaaaggtc
    10921 attgtctata tcaagctctg catcgcgagt aagctttata gtatatgctt caaatttatc
    10981 aaagctaaat atggcaaaaa ggtctttcaa gcaaaacctt ataatatcat caatcaggat
    11041 gatacattgt ctattgccat ctgatggtag ccttacaacc ctgtttagta tcgaaggtat
    11101 ctccatgagt acatattctg tttttacctt tctcttgctg ttgcttagtt tgatggcaaa
    11161 gtatattgat ttgtctttca tttcaggaaa gttctcaaca ttgctcagca taatcggcga
    11221 taaaaacgga agtatattgg tgtcgaagta attgtccaca aagtaagcct gttcttcgtt
    11281 tagttgttgt tcattgataa agtatatatt ttctttttca aattcattga tgatgttgtc
    11341 aaatgcctgg ctgaattttt gttgttggac caatactttt tcctgtattt gtgccaatat
    11401 cttagctggt ttctcgccac taagttcttt ggggttgtag gcgtagtcta tcatgcgttt
    11461 taaagtcgca acgcgtacct taaaaaattc atccaggtta tttgaatata tgcctaaaaa
    11521 ccgcatcctt tctactaaag gaactgtttc atcaatggct tcctgcaata cccgctcgtt
    11581 aaacgaaagc cagctcagat ctcggtttat tatttttttc tttccagaca taggtgttta
    11641 aattagtcgt tttgatggtg gtaaggtaat atcacttttt attggtattg aattaattat
    11701 tgaccagtct tttatcttaa atgtcagcgt tacaagcccg gaggtgggca aattttctaa
    11761 atattctggc aaaaacaggt ttacaaacct tgtaaatatc ggattatggc ctactattag
    11821 cagattgcta attttggctt ctaccgattc tactacttca agtactgtag aagagtctgt
    11881 ttcatacaat cgctcgtcta tcctcacttt gtgaggtgat acatttaata aacgtgccat
    11941 tattaaggct gtatgaatag cccttgctgc cgatgagctg ataataaggt ctggtggatt
    12001 atatttttca tgaaagagct cagctacctg cataacattg tttacccctt tggggagcaa
    12061 tggcctgtct atatcttcca gttccggata ttcccacgag gctttcccgt gccgtaaaat
    12121 atgtaaatgt ttaatctctt tcacttcaca ccccttgttc gtaaagctat tgggaaggtt
    12181 ggacttatag ctttctgact tgtaaagcaa agttaattaa cttcatcaaa agttgctggt
    12241 ttttagcttt ttttaatttt tattacaata tttaaagtgg ctattctccc aaatttctgg
    12301 tgtattcaat gaaaatatca ttttcatact ttttaagcat catattttct ccatcataag
    12361 tggcaaaagt attcgaaact atccattctc caaggtttat caaacgactt ttatcgttaa
    12421 gtttgtagtc caaggggatg tgcctgtgcc caaatacaaa aaaatctatt tcgttggtat
    12481 ttaggtattg tttggcaaat tgtatctggt gttccttgtc ttcgcccaga aaagcttccg
    12541 aaacaccttt tgacagccgg ctatgtttgc tccaggtatg acctatggcc agggcaaagt
    12601 tgggatgtag cctcgagaaa agaaattgca gaaatttatt cttgaaacac ccttttaaaa
    12661 agcggtaccc gtaatcagtt ttacaaagct cgtcgccatg gccaataaga aatcttttgc
    12721 cgcttatttc aaacaatcta tcggtataat gcacttgcaa cccaatttct ttgggcaaat
    12781 aatcgaaaac ccaaacatca tggttcccgg taaagtagtg tatttcaatt ccgctatcgg
    12841 ccaattcggc aagtttccct aaaaatcgaa caaagccgcg tggcgctact ttgcggtatt
    12901 cgtaccaaaa atcaaatatg tcaccttgta agaacagtat ttgtgcatct ttgcgaataa
    12961 cttcgagcca gagcacaatc attttctctc gctgggcgct tttatcgtac ggatataacc
    13021 ccaaatgcat atccgagcag aaatacgctt ttttgccttt ctgtagctca atatttatag
    13081 gtgttttact tgccaaggta cttctttatt tcggtttcaa gtttttcgcc aaaaacgttt
    13141 attgtcaaca ctcctttttc tttattaatg aggtatatcg aaggtataaa ctgcacattg
    13201 tatatactgc tatataccga attgcccacg ttttcatcaa ttacttggta tcctttaagc
    13261 tgcgctttat ctataaaatt actccaatat cctatatcat tgaataaagc aacattatac
    13321 acctctaacc ctttattacc atatctttca atcagattat ttatgctttt cattacaacc
    13381 acgcaatctg aatttgtagg tgtccaaaat agcaacaatg tcaacttcga tttttcaact
    13441 acctggctta acttgatggt atcgccatct tttgaaggca acttaatttc aggaaatgtt
    13501 cttacttctg cttcaagata tttgccttgc gatttcgcat ttttgagttg ttcaaatatt
    13561 cggtttcttt ctacccacaa tgcttttaca ggtttcgact ccggaaaata cttctttagc
    13621 gtatctgaag caagtttcaa atattgcatg tctcgaattt tccccatcac ataggtttgt
    13681 gaatctgttt gctggaacaa acaaataatg cttgaaggtg atcgaaggtg ttccaaaaca
    13741 aattttaccg aaaaactatg ctgttggtca taaattttct taatggctat attcatgcta
    13801 tctttagact ggttagtttt gcttatcgaa tctatagctt taaccgtgtt gttcaactgg
    13861 tcctgcaaca gttttgcttc ttttgaatat gctgagcctt ccacctgata gttttcataa
    13921 atttttgaag aaggacagtt tagatcaatt ttctcgcccg gggcaatggc caaaagcaaa
    13981 taattgtcgc gtgccacata tagtttgaat atgccagggg cctgtaattc tccttttaat
    14041 acaaatttcc cgttttggtc tgtttgggcc gaatctataa gtttttcaga agtaatgttg
    14101 tactcggtcc aatatatttt ggtattcttc catctttcca atacgccgtt tactctatat
    14161 ttttcttttg tacacgaggt aaaacaaata atagctgata gaaatatcac gcatgccttg
    14221 ccaatatact tttttatcat aataaatctc tatatgtatt agtatttaaa tatttcttta
    14281 agtctttctg ccaacttttg cgatgtaatc tgtttctcaa caattttgcc ttctttgtct
    14341 ataaaaagat tgaacggcaa ttcctctatt ccgaataacg caactaccgg agaatcccac
    14401 attttcagat ccgaaacatt gtgccacgga aacccattca cagtaatagc ttttagccat
    14461 gcatcacggc tcttatctat gcaaacatgg taaacatcaa agcctacatt cttgtatcgc
    14521 cagtattgtc ctataaattc tttacttgct tctatacttt tctggtttga tgaagcccaa
    14581 aaaaggacta atacgtattt cccccttaaa ctgcttaatg ccaaaggctg attgttgaaa
    14641 tctggcaaat ttatttctgg gatgggtgcc cccttggtaa gtctttgttc tatctgtacc
    14701 ttacgtatgt attcctctga aatgttacca actaaactgt gtaacgattg tactgcatct
    14761 gcttccggat aagtttgcat cattaccgaa tctacctctt taaatatagc ataatcattg
    14821 gccggcgtaa ataatgattt tcttggcgtc atttgttggt acaatgccat aaccgaggct
    14881 agcgtccctt tgttttccct aataaaattg atagaaaaac ttttttggtc actctcaatg
    14941 attttatata ccgaatctaa ctccgatttg atttttaaaa gatttttact tcctatgctg
    15001 tcatggaatg ttttgcccaa ctggtttatc ttttctaagg tctcgttaag ttttattgtc
    15061 agtttcttca ctaactgtga ctcttccgag ccttttacat catattcgtt cggcaaatct
    15121 ttagcatctg cctgcacaaa aatcttgtcc cccggtttta taaccaatgt aagcgtattc
    15181 ttcttgtcta agccaagtgt ataaaatgct attttattat ttatgccttt taaaacaaaa
    15241 ctaccatcgt tgtcaactat tacagagtct attgttttta tatcagagat ggtaagctct
    15301 tttaaaaaca gcatctcccc cgatgcatta cgcagtttgc ccttaacttt aaagctttct
    15361 tcgttactgc atgatgttac aaacaaccct attgtcaata tccccaaaag aaaattcact
    15421 tttttcatgt agcttgtatt catttaaatt ctggacaaat ttatttttta tttcgggttt
    15481 gacgtaattt acttctaaac ttattttttt gaccgaatct aaattcttat agaccaatac
    15541 gtgtttatga gtgataaatc atcgtttttg tttgatagtt atatctcttc tgaatttata
    15601 agatattcat ttagcggatt ttacaagaaa tattaaacat taaaaaaata ttaatttatt
    15661 tttttcaact tgacaggtaa cgtaatattt cataacttta aaaatattaa aaatattaaa
    15721 catctaataa aattggcatt gtaattgttt ataattcaat tttatacaaa ccttaaaatc
    15781 tatgagctac cttaaatttg acaaaacaca gttagttaat cttgagtact cgcttaccaa
    15841 agaaattgtc cgttcaaacc gtgcaggctc ctactctcac actacaattg ttaactgtaa
    15901 cacccgtaaa tatcatgggt tgttggtttg cccgatagaa gaaattgatg gagaaaaaca
    15961 tgtcatgctt tcttctcttg atttgactgt tattcaacat gacgcagagt tcaatctggc
    16021 catacataaa tatcctgggg atgtatatta tcctacaggg cacaaatata tacgtgattt
    16081 cgaaattgga aaagttgcat taacacgcta tcgcgttgga ggagttgtta ttacccgcga
    16141 atctattctg gtcgagaaag aagaacagtt gatactgaaa tataccttgg aagaagctca
    16201 ttcccctaca actttgagat ttaaaccatt tttagctttt cgcaatatcc attcccttag
    16261 caaggccaat atgtatgcca ataccagggt agaaaatgtt gccaatggca taaaatcaag
    16321 attgtacaac ggctatcccg ctttgtacat acagttctcc aaaaaggtag aatttattcc
    16381 tgctcctgac tggaattata atattgagta ccccgaagaa caagcccgtg gatatgatta
    16441 ttcagaagac ttatttgttc ccggctattt cgagatgcac attaaaaaag gggagtctat
    16501 atacatttcg gccagtttgc atgaagccca gccggcatca ttgtccaaaa tatacgcaaa
    16561 agaagttaat agccgcgagg aacgcaactc ctttcatagt tgcctcaaaa atgccgccaa
    16621 acaatttttt gtcaaaaaag acaaaaaaac cgaaatcact gccggcttcc cctggtttgg
    16681 cacctggggc cgcgatactt tcatttcatt gcctggattg actttattta ctgacgacga
    16741 aaaaattttc aagtctgttg tggatactat ggtggacaga atgaaaaacg gccttttccc
    16801 aaacatgggc tcagatagca acccggcctt taattctgtg gacgccccaa tgtggttctt
    16861 ttgggcattg caacaatatg tcaacaggca caattctgct gccgaaattt gggaacgcta
    16921 tggcaaaacc atgaaaacaa tccttgaagc ttatcgtacc ggaatctctt ataatatcag
    16981 aatgcacgaa aatggcctga tatgggccgg agaacacggc cacgccctta catggatgga
    17041 tgctgttgtg gccggtaagc ctgttacacc ccgcatgggc tatgatgtcg aaattaatgc
    17101 cctttggtac aatgcgatat gtttttgtct cgacctggcc aagcttggca aagatacccg
    17161 ctttatcaac acctacaaac acctgcctga actgattgaa aaaagcttcc tcgaaacctt
    17221 ctggaatgaa gaaaaaggct atctggccga ctatgtggac aatgaacata cttgttggca
    17281 agttcgccca aaccaaataa ttgctgcttc tattgactat aaccttattc cagatgatat
    17341 aaaacaaaag gtcattgaag tggtgaaaaa tgaattgctc accccttatg gcttacgtac
    17401 tctaagtcct aatgatgagc attacgaacc tgtttatgaa ggcgatcaac ctgcacggga
    17461 caaagcttat cataatggca cggtgtggcc ttggttgttg cagccattct gtattgccta
    17521 tatcaagctt tataaaaagt ctggcgtacc gttactcaaa cgtatatacg aaggttttga
    17581 acctatactt actgaatacg gagttggcac tattggtgaa atatttgatg gcaaccctcc
    17641 tcacaaacct cgtggcgctg tttcgcaggc ttggagtgtt gctgccctga ttaccattga
    17701 gcaactcatt aatgattatg ataaataatt gatgtaaaac ctctaacctg cacataacaa
    17761 gtttttttat taacctactt ctaaaaacac ataattcatg aaagtattaa tgtttggatg
    17821 ggaatttccc ccacatattt caggaggcct gggaactgca tgttatggct taactaaagg
    17881 cctttcaagg cacaatgacc ttgaggttct ttttgttgtc cctaaagctt ttggggatga
    17941 agataaatct gcagcccata tcattggggc ttctgacgtt tatatcaacc agaaaaagat
    18001 caatataaag ggctttttga aagaagtaaa catgattgag gtcaactcca cattgatccc
    18061 ttatactgac cttgacgaat attacaacat tatccaaaag tataatttgc ataataataa
    18121 gaatgttgta aaatctgact ttgaaggccg attcaatttc tccggaggtt atgggcctat
    18181 gctttttaac gagatttaca actattctct ggtagcctct atgcttgctt ccgaacatag
    18241 ttttgatatt attcatgccc acgactggct tacttatcct gcaggtattg tggctaaaga
    18301 aatttccggg aaacccttgg tgatacacgt tcatgcaaca gacttcgacc gaagcggtgg
    18361 caacgttaac ccccgcgtct ttgaaattga aaaacgcgga atggaagtgg ccgataaaat
    18421 tattacggtg agcaactgga ctcgcgatat tgtcattaac aaatatggca ttaacccgaa
    18481 taaggttgtt actgtttata acgccgttga accattaaac gaagccgaaa cctcaaggta
    18541 cgaaaaatat cttgacgata aaattgttac tttccttgga cgcattacca tgcaaaaagg
    18601 ccctgaatat tttatcgaag ctgcacgtaa agtactcaac cgcatggaca atgtacgctt
    18661 tgttatggct ggtagtggcg atatgatgaa taaaatggta ctgcgtgttg cccaacttgg
    18721 cattgccaac aggttccact ttacaggctt tcttgccggc gacgatgtta acaatatgta
    18781 cgcattgagc gatgtgtatg tcatgccttc tgtttctgaa ccttttggca tttcgccgct
    18841 tgaggctatt cgctccaatg tgccagtaat tatctcaaaa caatccggag ttgcagaggt
    18901 gttgaaccat gctataaaag ttgatttttg ggatattgat gccctggctg acgctatata
    18961 tggtattctc aattatcctg ccttatcaaa gatgtttaaa acatacggaa aagaagaagt
    19021 tgataatctc aaatgggaaa attcagcaga tcatgtccgc gctatttatt accaaactat
    19081 ttctgcctaa tcttaattat gaataataat aacttaatat gaaaactatt tgcttatatt
    19141 ttcaagttca ccaacctttc cgtttcagga gataccgctt tttcgaaatg ggcaaagaac
    19201 actattacta tgatgattat tccaacgaaa gcatcatgcg taaagtggcc atgaagagtt
    19261 acttgcctgc caacaaaata ttacttgaaa tttttaaaga atacggttgc cgctttaagg
    19321 ttgctttttc tatctcaggc acagccctcg accaaatgga gctttatgcc cctgatgttt
    19381 tagaaagctt ccaaaaactt gccgcttcta attgtgtaga atttttgtca gaaacatatt
    19441 ctcatacctt agcatcactt attaatcgtg aagaatttga aaatcaggtc aaagaacacc
    19501 aagagcgtat ccttaaatta tttggcaaaa aacctgtcgt gtttcgcaat actgagctca
    19561 tctataataa cgaaatagga gagtggattg ccgatatggg ctataaagca gctattattg
    19621 aaggggctaa acatatcctt ggatggaaaa gcccaaacta cgtatattgt agtgctgtta
    19681 atcctcgcct taaactgttg ctcaagaatt acatgttatc tgacgaccta actttcaggt
    19741 tctctgatgt tcattggaac caataccccc tgactgccga aaaatttacc tcctggatta
    19801 aacacatgga tcaaaaagaa gatactctca atcttttcat gaactacgat acttttggaa
    19861 atatccagcc aaaggaatcc ggcatctttg aatttctaaa agcttttccc aaagaagtat
    19921 taagcaccaa taccttgagc tttagcaccc cttccgacct tatcgaaaaa ttccagccaa
    19981 tttcagctct ctcagtccct cacgctattt cctgggctga cgaagaacgc gatacttctg
    20041 catggttagg taacgaattg caaaaggagg ctttttataa acttttcaat cttcgcgaca
    20101 tggtattgca aaccaatgac cccaaaattc ttaccgattg gaagtatttt caaaccagcg
    20161 atcattttta ttacatgtgc accaaattct ttgtaaaagg cagcatgcat tcgtatttca
    20221 accctttcag ctctccttac gatgctttta tcaactacat gaacattctt agcgattttg
    20281 aaattaaaat caaactcgca ttatcttcaa ataattcagg cattaaaaaa acaaatggca
    20341 atggtactaa gaaaacaaat aatatagctg ctaaaactga caaagaaaaa gtgaaaaagg
    20401 tagttaaaaa aactactgca aaaactgcaa aaccaacaaa agaagatgta gttgtcgaaa
    20461 aaactaataa aaagacagct aagaaagaaa caactaaaga aaaagtaaaa aaatctaaaa
    20521 aagcgtaata ttttaacctt aaaaatgtat gagtaacaaa acaacgatta tgcccgacta
    20581 tctgtttgaa gtaagttggg aagtgtgcaa taaggtagga ggcatttata ctgttgtgtc
    20641 aacaaaagca ttgaccctgt cgcaacaact tggtagtaat tatatcctca ttgggcccga
    20701 cgtttggcgc gatggagtcg ttaatcccga atttgttgaa gataaacgtc ttttcaaatc
    20761 atggagggac aaagccgccg aagaaggcct gcgtattaaa gtcgggcatt ggaatattgc
    20821 aggcaatcct gtggccatta ttgttgattt taccactttt attagtcaga aagatacaat
    20881 tttctctaag ttttgggaaa agtataaact cgattctatt accggccagt gggactatat
    20941 tgaaccttgc ttatttggat atgccgcagg aagggtgatt gagagctttg caaaatttaa
    21001 cctcagcgtc cgtgatagaa ttgttgccca gttccatgaa tggatgactg gtagcggttt
    21061 attatatctt aaatctgaga tgccccaaat aggtactgtc tttacaactc atgcaacagc
    21121 tatgggacgc tctattgctg gcaacaacca acccttatat tctaatatta aagactatat
    21181 acctgaccta aaggctgctg agtttaatat tatttcaaaa caatcgcttg aaaaaaactc
    21241 tgcccaacat gctgattgtt ttaccactgt gagcgaaatt accaaccttg agtgtgaagc
    21301 ttttctgggc aaaaatgtag atgttgttac cccaaatggt tttgaagact cttttgttcc
    21361 aactgccgat aaatttgacc aggcaagatt gcaagcccga aaaaaactta ttgaggtggc
    21421 atctgcctta actgattcta aaattaatga cgatgttttg ttgattgcca atagcggacg
    21481 ctatgaattt cgcaacaaag gtattgactt gtttattgat tcgctcggag aaataaataa
    21541 gtctggacac ttaaacaaag aagtagttgc atttatcctt attccggcaa accactatgg
    21601 gcctcgccgt gatttagcta ataaactcaa aggacaggaa tatgaacatt gcgatgagtg
    21661 catccttacc cacaacttac actatcctga gcacgatagt atcctgaaaa gaataaaagc
    21721 aaacggatta acaccttctg aaacaaataa ggtaaaggtt atatttgttc cttcatatct
    21781 caatggagat gatggtatat tcaatatgtc atattatcaa ttgcttattg ggtttgacct
    21841 tactgtattc ccctcttatt atgagccttg gggttacact cctctcgaaa gtattgcttt
    21901 taaagtacct acaattacaa cctcattggc aggctttggc ttatggataa agcagctttc
    21961 tatgagttct gaaaatgcag ttactgttat tgaacgcacc gatataaatg atgctgaggt
    22021 tactcaccaa attgcaggac agatattgaa attttctaac cttaatgccg aacaggtaga
    22081 aaatgcccgt aatctttctt ttgaaatgtc tcgtaccgct ttatggaaaa acttggtttc
    22141 atattaccta aaagcctatg agattgcctt aagtaaagtt caagaacgaa caagcacaat
    22201 tgctgaaact gaaaaaatcg aaacccttac cgctgctgaa aaattttcag taactaatga
    22261 ccctgtatgg aaacgtctcc ttgttcaaaa aaatattcca gaagaacttc acgctttgga
    22321 agaattatct aaaaaccttt ggtggagctg gaattatgac gctattgacc tattcaggtc
    22381 tattgacccc gaattgtggg ttaaaagcga tgaaaatcct atagtcttgt tagatcttat
    22441 tccttacgag aagatcttga agctttcaaa agacgttgtt tttctcaaaa aacttaatga
    22501 catttataat aagtttcttg aatatcaaaa caaaccatgt aaagttggcc ccaaaatagc
    22561 ctatttttca atggaatacg gtttgcatga ctctattaag ttgtattcag gaggacttgg
    22621 ggttttagct ggcgattatc ttaaagaggc cagcgatgcc aatattgata ttattggagt
    22681 tggtttgtta tacagatatg gatatttcaa acaagtaatt tctgaacatg gcgaccagat
    22741 agcccaatat gaatctcaaa aattttctca aactcctgtt cagcctgtac gcgatgccaa
    22801 tggacaatgg ttgaccatac acatcaacct tcccgggagg cctcttattg ctcgtatttg
    22861 gcgattaagt gttggccgta tccctttgtt cttgcttgat accgattttg aagataatat
    22921 cgaacaagac cgcagtatta cccatcattt gtatggagga gactgggaca accgctttaa
    22981 acaagaattg ttgcttggcg ttggaggtat cagggctttg gatacactgg gtatcagtcc
    23041 ggatttattt cattgcaacg aaggccatgc agcttttatt ggggtcgaaa ggctaagaaa
    23101 atatatagtt gaccaaaacc ttacctttgc agaagggctt gaattggttc gatcttctca
    23161 gttattcacc actcataccc cggttcctgc cggacacgat gcttttgacg aaagtatgat
    23221 gcgtacgtat atggggcatt atcctaaacg cctcaaaata tcatggttac agtttatggc
    23281 tcttggcaag gcccatcctg ataatccttc tgaaaaattt tccatgagcg tgcttgctgc
    23341 taacctttct caagagataa acggggtaag cgagcttcat ggacgtgtta gccaggaaat
    23401 ttttcatgac atgtaccgtg gttatgccgc cgaagaatta aacataggat ttgtaaccaa
    23461 tggagtgcat atgcctacct ggacagctcg tccttggatg gagttataca aaagcacttt
    23521 tggagatgat ttttttgaaa agcaaacaaa gtttgaaacc tgggctaaaa ttcaacaagt
    23581 acccaatagt aacatttgga aaattcgaaa ctctcaacgt caaatattgg tcgattatat
    23641 aaaagacagg ctcaaagatg ctgcaattaa acgtctggaa aatccaaaaa tggtagtaga
    23701 aatgtgcgac cgtttaaaca aacacaacct taccattggt tttgcaaggc gttttgctac
    23761 atacaaaagg gcacatcttt tatttaacga tattgaaaga ttgtcgcgta ttgtaaataa
    23821 tccatcgatg ccagtgcagt ttgtatttgc tggcaaagct catccccatg ataaagctgg
    23881 acaagatttg ataaaactga tttctgaaat tagtaaaaaa ccgcaatttg ttggaaaaat
    23941 tatttttata cctaattacg acatgaagct tgctaaaata ttggttcaag gggtcgatat
    24001 ttggctcaat accccaacaa gaccattaga agcttctggt acaagtggcg aaaaagctgt
    24061 tatgaacggt gttttacact ttagcgtcct tgatggatgg tgggcagaag gttatgttga
    24121 tggtgcaggt tgggctttgc ctgaagaagt aacatatcaa aataatgctt atcaggatca
    24181 attggatgcc gaaacaattt ataacctgat tgaaaatgaa atagccccac ttttttattt
    24241 cagagatgaa aatgggctcc ccaatgggtg ggttcagtat attaaaaact ctatcgaaaa
    24301 agtagccagt cactttacca tgagccgcca gttagaagat tacaatacta aattttatac
    24361 aaagctttac gaccgtacgc ttaaagtgca agccaacgac tatcagttag ctaaagaaat
    24421 ggctcgttgg aaaaagaaag tccgttcaag ttgggaaagc atcgaggtgg ttagtaccaa
    24481 tattccagat gtttcccgcg aagcaattag ccttggcaat agttatgtgg cccaaataga
    24541 acttgacctt aatgaacttg ctccagaaga tattggcgtt gaacttgttt tagctgattt
    24601 ggttggagac gaaagcaaac ctaaagtttc ttattgccaa gaatttgaat tggtcaaata
    24661 tgatggacaa attgctcaat atcgtattga aatgcggccc caaaaaacag gggtatatga
    24721 atatggaata aggctattcc caaaacatcc tgagttgcca cacagacaag atttcaatta
    24781 tgtgcgatgg ctcgaatagc agattatcta aaaatcccgc ataagaaaaa caccgtctga
    24841 aatttcccct tgcagggggc agacggtgtt tcatttttta caaatactgt tggcttagat
    24901 aagccttctt aagctctcgt cagctttctc tgtatagcaa gcttgctttc tgatactaag
    24961 ataggtaatg tctcccccta atgccagctt tttaacattt ctttttatca agaaaacctg
    25021 attttactgg catttgtctt attcaaacaa tatatttgta gtttatatct cgtttttggg
    25081 caaaaaaaaa gccaactctt ggttggcttt ttttgtttat aatagttatt gaatagctct
    25141 tttttcctga atacgggcct tcttaccagt taaatccctt aaatagtaaa tacgtgccct
    25201 gcgtacttta cctactttat taagttcaat tttatcaata aaagcagatg caataggaaa
    25261 aattctttct acaccaatgt ttcctgacat ttttcttaca gtaaatgttg ctgtatggcc
    25321 tgttcccttt ttctgaataa ccactccttt atattgctgg atacgttctt tgttaccttc
    25381 tttaatttta tagtgaacag taatggtatc gccactttta aatgctggaa actcagtttt
    25441 tgcagccagg ttttctttta caatgttcat caagtccatt atcgtaaatt tttatcgttt
    25501 ttgctctgaa aaataggctg caatattaca aatatttttt aatataaaga gcaataaaaa
    25561 gaaatttctt aaaaaatctc tttagcctct tgttttatat agtctatagc cttttgcaaa
    25621 ggtaatttat ctatattact gatatcttct aatatttttt tacttaagtc aatgcccgga
    25681 gctttagggt cagtactttc agcagcaaga ttgataattt tcactatctc agcgcgggca
    25741 tttttaatca tctcccaagc ctttgccgat acatataatt gttgcgaaat attgtgttca
    25801 aattcttttc ttattgaact taataattca gtatgtaatt gtctgctggt aataccactt
    25861 ctgctatgac gtgtaagcaa catgtctatt gaaatacgtt caagaaataa tataagcctt
    25921 tcgtatgcct gcaaacgcaa aggcatcatc atttcatgag tctttaataa caactccacc
    25981 tttctgtttt tttctgcatt acgaagaaaa agccagacca ggtagatgtt tgtgccagaa
    26041 acaataatga aaaagctaat tattataatc cattcagtta tattcattgc ttaaattatt
    26101 tatttatcct ctgtggattg tttgcgtgaa agaactccca agtaccctcc atgatggcgt
    26161 tttgcataat cctcggcagt aaagttaatc tttttcatca attgcactac caaaacatcg
    26221 cctatcacag tcatggcagt ggttgaggtt gtgggggtaa gccccaatat acatacttcc
    26281 ttcgggttgc ctgtagataa agtcacctct gcttctgaac ttagctcccc atttggattt
    26341 cctgtgataa ggataatagg aatgcccggt tgtaaacgcc ttgccaaatg tgttagttct
    26401 ataatttctc tggttttgcc tgaatttgaa attaatataa gaatatcatt ctcctgaata
    26461 atccccaaat cgccatgctg agcttccgaa gggtgcaaaa atactgcagg tgtaccagtc
    26521 gaactaaaag ttgtagccat gttcaatgct atctggcccg ctttgcccat gccactggcc
    26581 acaagtttac ctctttttac atgtactctt tcataaatca gatcaatggc tttttcatag
    26641 cagctgtcaa tagggatgtt taatattgat tttgcttcgt gctcaagtat ttgttttatc
    26701 tcgtttatca tttatccaaa atttgaagca atgttattgc cattttttca attatcaaaa
    26761 tgtctttcgt ttttttgtac cacaaaaact attatgcact ttttcaactt tccttatctt
    26821 tgccgttcaa aatttttgtt gaatggaaaa aatcgaaagt aacctggttg catcggttat
    26881 taaagctgtc ggatctttat acaataaaag cattgagcca aattttgtgc aaatccagaa
    26941 aacccgtaaa gattttaagg gagatatcac tattgttgta tttcctttgt tgaaattttc
    27001 tggtaaaggt ccggaacaaa cggcaaatga cctgggcaat taccttgttc agcattttga
    27061 gcttgttgaa gggtttaatg ttatcaaagg ttttttaaac attgaaatta gtaaacaata
    27121 ctggttttct tttcttgaag atatttattt aaccaagggt ttagaagggg ggcaactttt
    27181 tcatacatcc tctcctaaaa aaattatggt tgaatattct tcgccaaata ctaataaacc
    27241 tttgcactta ggtcatattc gtaacaatct tcttggatat gctgttgcca atatcttaaa
    27301 agctactggc aacgaagtgt tgaaggtaaa ccttatcaac gacaggggta tccatatttg
    27361 taagtcaatg cttgcctggc aaaaatttgg caatggggca actcccgaat ctaccgggat
    27421 taaaggtgac catttagtcg gtgattatta tgtccgtttc gaccaggaat ataaagccca
    27481 gactaaagag ctgatgtccc aaggcatgac tgaagaagaa gctaaaaaac aggcccccat
    27541 tctccttgaa gctcaagaaa tgttgagaaa gtgggaagcg ggagaagaag aagtcctttc
    27601 tgtttggcgg atgatgaacc aatgggtgta taacggattt gatgaaacat acaaacgact
    27661 gggtgttgat tttgaccgtc tttactacga atctcaaaca tatttattag gaaaacaaat
    27721 agtgcttgat gccttgacca aaggtctgtt gtacaaaaag gaagacggct ctgtttgggc
    27781 cgatttgact gaaaaagggc ttgaccataa ggtactgctg cgttctgacg gaacatctgt
    27841 gtacatgacc caggatatag gtactgccca tcaaagattt gaacaatatc atatagatag
    27901 ccatatttat gttgtaggaa atgagcaaaa ctaccatttt caagctcttg ccatcgtttt
    27961 agggaaaatg ggctttgact gggccgataa aatatttcat ttatcttatg gcatggttga
    28021 gttgcctgaa ggtaagatga agtctcgcga aggaaccgtt gttgatgcag atgatttgat
    28081 ggacgagatg attagtactg cccgccaaat gtctgaagaa cttggcaaat tagaaggctt
    28141 atccgctgaa gatataaata aaactcttaa tatcattggg ttgggagcct tgaaatactt
    28201 tattctcaaa gtagatccta agaaaaatat ggtttttgat cccaaagaat ccattgattt
    28261 caatggcaat accgggccat ttattcaata cacttatgcc cgaatcaaat caatacttcg
    28321 aaaagccgcc gatgcccaaa ttgtttttga tttaaaacaa gcttctgctg taaacatggg
    28381 agacaaagaa aaagaactgg ttaaactgct ttacgatttt ccaatggtgg tgcaagaagc
    28441 agccaaagaa ttttctccgg ctattattgc aaattattgt tatgatcttg caaaagaata
    28501 caaccaattt tatcacgatt gcccaattct caaagaagca gatattgcgg tcaagagttt
    28561 acgatttgca ttgtcccaac aggtttctca ggcaattggt tatggaatga agcttttagg
    28621 tattgatgtc cccgaaagga tgtagttaat tagccctttt tttaatcggc tttttattaa
    28681 aattgttgta actttataca aagttgagaa accttagctt atttttaaat aacaaaaaaa
    28741 aaatttgata aataattttc aattcaataa atttgccgct catttttaaa aagacaaaat
    28801 atgattatcg tacctattaa agaaggtgaa aaccttgaac gtgcactcaa aaaattcaaa
    28861 cgtaagtttg aaaaaacagg aactgttaaa gaacttcgtg agcgtcaatt cttcaccaaa
    28921 ccttcagtag aaaaaaggca ggaaataata cgcgctatct acagacaaaa aatgcaagca
    28981 ttagacgagt aacatccagc ttgcagtttt ttcatttaaa actgttatat gctgtacatt
    29041 gatgaatatc taaattattt aaagtttgaa aaacgatatt cgtctcatac ggtgttgtca
    29101 tataataatg atttacagca gttttctctt ttcattgatt gtgaaaatcc tgaaaacata
    29161 aaacagcagg atgtccggca atggataatc agcctttatg atgcaggaat atcgccaagg
    29221 tcaataaata gaaaacttac agcattaaaa gcgttcttta aatttttgtt gaaaaatggt
    29281 aaagtagcta aaaaccctgt aattgggatt aaagcgctta aaacaaaaaa aagtttacct
    29341 cagtttgttg aagaatcaaa aataggctct cttttagagg ataattattt tgaagttgga
    29401 tttgaagggt atcggaataa gattatcatt gaaatgtttt attataccgg cattcggctt
    29461 tcagaactga taaaccttaa aacttcagat gttgattttt tcaaacaatg cattaaagtg
    29521 cttggcaaac gaaacaaaga aagaataata ccattaaccc caaagtttgt ttcacattta
    29581 aatgaatatt ttaaaatgaa aagtgagtca ggggttgcag attctggttt tgtattttgc
    29641 agtagtaaag ggaaacaatt aaatccgcgt actatttatc aggtggtaaa cacctatctg
    29701 aaaatggtta ctagtattga gaaaaaaagc ccgcatgttt taagacatac ttttgccacg
    29761 catatgctga ataatggagc agaccttaat gcaattaaag aattgttagg gcattcaagt
    29821 ttagcagcaa cagaagttta tacacataat acatttgaga agttaacgaa tatttataaa
    29881 caagcccatc cccgggcata aaaaacggag gaagagctat gaaaataaaa ctacaaagcc
    29941 tacattttga tgcagatcag aaacttatcg attttattga aaaaaaagcc gataagcttg
    30001 caaaattgta tgaaaacatt gttggtatcg aagtgatact aaaactggac aatgattcga
    30061 aagacgaaaa taaggttgtt gaaatgaaag tagaaatacc cggcaatgat cttttttcga
    30121 aaaaacaatg cagaactttt gaagaagctg cagaccagtg tgttgatgca ttaaaaaccc
    30181 aacttgtaaa attcaaggaa aaacaaagag gagaataaga aaaattctta gttttttttt
    30241 gaatagtaaa taaaaagtta tacatttgcc aacccaaaac aaaaggggat aggtaatgca
    30301 aaaaaggagt attgccaagt gtttgtatag ttcattgaaa ttattcatta tatcatgtga
    30361 tatgttgccg atgtagctca gctggttaga gcagctgatt tgtaatcagc aggtcggggg
    30421 ttcaaatccg tccatcggct catttaatta atattgggga gattccagag cggccaaatg
    30481 gggcagactg taaatctgtt gtctgatgac ttcggaggtt cgaatcctcc tctccccact
    30541 taagattgat aaatgaccat taatgattaa aaattgaaat tcaattcaac aatcataaat
    30601 tttcaattgt caattatatg cgggagtagc tcagttggta gagcataacc cttccaaggt
    30661 tagggtcgcg ggttcgagcc tcgtctcccg ctcatagctt ataggtagta ttatatactg
    30721 caaatgagta aaacggaaga aatgaaaagt gccatttaag ctcagggggg aagggcatgc
    30781 cgaaaattcg gaaaaggaag tctccaaagg gtgaaaaggc taaaataaaa tgccgttgta
    30841 gctcaggggt agagcacttc cttggtaagg aaggggtcat gggttcaatt cccatca
//
LOCUS       DAOSPF010000040        30838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1496_length_30838_cov_7.394211, whole genome shotgun sequence.
ACCESSION   DAOSPF010000040 DAOSPF010000000
VERSION     DAOSPF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_30838_cov_7.394211"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..411)
                     /locus_tag="PK252_09000"
     CDS             complement(52..411)
                     /locus_tag="PK252_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14882.1"
                     /translation="MKNYITSIILLFIFISYGSAQNADVVYGKLMYKSSNATYAIASA
                     KILLCSGKDTLSAFTDSEGNVAFYNVKVKTYLLKAVMNNDTLSFIQPNKPKTKALSVV
                     VKAGSNNFGKINAVIKR"
     gene            complement(443..1006)
                     /locus_tag="PK252_09005"
     CDS             complement(443..1006)
                     /locus_tag="PK252_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14883.1"
                     /translation="MNPQLSIVTSDPKMFVLVVFSLSFVLSMVAAFVLFKFLQSTAVI
                     NNKNYQAGGALAGFIIVLGASYMMINNLLKTTSTIDQPQPQVVENTWTLVGNVKLVGK
                     NVNDGIKVSIQPPSPTTSTDFNGDFRMDNISTSHGFSKKSNPAIKLEYPGYLPRTVYL
                     YDTAMIVADSAKFTFSLKSGVSLIAIQ"
     gene            1211..4024
                     /locus_tag="PK252_09010"
     CDS             1211..4024
                     /locus_tag="PK252_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT14884.1"
                     /translation="MNRKRIIQALAMVALVLASCNVKKEVKNIQNGVVVSTSDVNIKV
                     QFYAPNMARVQKWLPAGSDKKLSLVIVMDSIPSVTVKVTEEKNLVTLESESLKITINA
                     KDGAVAFLDLNNTPFVKEADYSFKPVVYSDDSAYTVEQKFELTADEGIYGLGQHQSGY
                     MNYRGKKVLLSQANTEAVTPVFVSTRNYGILWDNYSLTEYRDNGKAEASMWSEVSDNI
                     DYYFIAGNSLDNVIAGYRNLTGVAPMYGKWAYGYWQSKEHYDNQNEITTVVNKYRELK
                     YPIDNIIQDWDYWNGTQNWGSMQFDKTLFPEPEKMVKDFHAKNFHMMISIWPAMGSNT
                     EVYKDMLAKGFLYSPVGWAGFKYYDAYNPDANKLYWEYLKKGLVSKGIDAWWMDSTEP
                     DVVNALTKEAENYEMKKMGKCYLGTFARYLNPYSLAMTENIYKNWRKDVPGKRAYILT
                     RSTFAGQQRNAATTWSGDIGANWQVYRNQISAGINHSMSGIPYWTFDIGAFVLGAYEG
                     VFNTGGKNPAYQELYTRMFQLGAFCPIFRSHGSETPREIWEMGDFTPTLLKYDNLRYR
                     LLPYIYSLAWKVTNEGYTIMRGLPMDFSTDKNTYNIDDQFMFGPSILVSPVTDYMYHC
                     PPQASIKVGEEFFKTKDGKPGLQASYYKDNKYKTLGKQQVDKNIDIFWYTGRPDYVTD
                     SMYAIRWEGKLIPKESGKFQFHMKCFDAKRIILDGKQLPIVYTSTEQYTDTLHLQAGK
                     EYSIVVETENTSTGAARMQLFWKTPSIFAAEKAKVDREKKRTLYLPQAKGWVNFWTGE
                     TANGGQKITTDAPIDIMPLFVKAGSIVPMGPFVQYATEKPADPIELRIYKGDNGSFVL
                     YEDENDNYNYEKGIYSTIRFDWDDASKTLTIGKRQGVFPGMLKHRTFEIVLVSEKHGT
                     GLDIETKPDKVIKYSGEELKVKL"
     gene            complement(4462..5868)
                     /gene="ahcY"
                     /locus_tag="PK252_09015"
     CDS             complement(4462..5868)
                     /gene="ahcY"
                     /locus_tag="PK252_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT14885.1"
                     /translation="MSNTLKYKVKDISLADFGRKEIEIAEKEMPGLMAIRQKYASSKP
                     LKGARIMGSLHMTVQTAVLIETLVYLGAEVRWVSCNIFSTQDHAAAAIAAAGIPVFAW
                     KGETLEEYWWCTDMALRFENGLGPNLIVDDGGDATLFVHWGFKAENDASVLDRKTSNN
                     EERIILEQLKKSLAEDKTRFHRIVSDFKGVSEETTTGVHRLYQMLEKGELLFPAINVN
                     DSVTKSKFDNLYGCHESLADGIKRATDVMIAGKVVVVLGFGDVGKGCARSMRSYGARV
                     IVTEIDPINALQAVMEGFEVSTIEEALAEGNIYVTATGNCDVITLEHMKKMKDQAIVC
                     NIGHFDNEIQVDKLVNMPGIKHTNIKPQVDKYTFPEGHSIFLLAEGRLVNLGCATGHP
                     SFVMSNSFSNQTLAQLDLWANKYEIGVYRLPKKLDEEVARLHLGQLGVKLTKLTQAQA
                     DYIGVPVDGPYKPEHYRY"
     gene            complement(5865..6803)
                     /locus_tag="PK252_09020"
     CDS             complement(5865..6803)
                     /locus_tag="PK252_09020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOT14886.1"
                     /translation="MTTHYFSDLDKSKFIRPVITVGIFDGVHKGHRYIIDQMQRQAVL
                     NAGQTVIITLWPHPRMVLYPGKEIKLLSTLDEKIELLESSGVDHLVLVPFDKGFAALK
                     ANEFIEKVLVEKIGVQSLLLGFDNHFGNNKEGNYEVVRQASLKYGFELLHPAPLFDGR
                     DSVSSTDIRLYLELGEIEKANHLLGYNYFITGKVVEGQKLGRTLSFPTANIVTDEFKM
                     LPRVGVYAVKVKTGYGIFNGMLNIGFRPTVDHNSLHKTIEAHLFDFNNDLYDTIITVT
                     FVARLRDERKFASIDDLRGQLEKDRTDSINILKHIS"
     gene            complement(6853..7818)
                     /locus_tag="PK252_09025"
     CDS             complement(6853..7818)
                     /locus_tag="PK252_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14887.1"
                     /translation="MERKILLFLVIALGFNLYGQQDSLWNKDIIKKNKIRTVTFYEYD
                     YVNGKPETDGVKTRVDSFDTNGQRVLQTYYRENGLVLSISTSKYDSKGNRVFQTKQAD
                     IDGRIKTVFIQDVRFDSKGNKTLEYGYNGIDSFKNVYNYNKYGKLAEVNFFIKKRLDE
                     KRVFTNNSNSSDLKILDGYGSLKYTQKYLFNSNDKVLQESTVMPDNTESQRLEYTYDS
                     KDNMLSETKYLKGKLIYKISYVYKGGLLSEVYKEGGDGIKFLVAKYTYDAAGNVSEEQ
                     TREDAKKDFSKSVYTYDKNNLCKTIDSYFAKFNKQVLTVIIYQNY"
     gene            8010..10031
                     /locus_tag="PK252_09030"
     CDS             8010..10031
                     /locus_tag="PK252_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOT14888.1"
                     /translation="MSQKTPVIVQNDTWLAPFRDIIVHRNELVNKKIKKLAPTGSLKD
                     FALGHLFYGLHKQNDVWVFREWAPNATEIYLVGDFSKWLIDKQFALRKIGNGNWQLEV
                     PSDYIRHGQQYKLLVKWSDGEGYRLPSYANRVVQDEATKIFNAQVWMPDKAYQWKNNF
                     IPSWDFPLIYEAHVGMSSEEPKVSTFREFENNVLPRIKKLGYNALQLMAIQEHPYYGS
                     FGYHVSNFFAVSSRFGTPDDLKSLIDKAHEMGIAVIMDLVHSHAVKNVLEGLGLFDGN
                     PGQYFHTNDRRIHKAWDSLCFDYGKNEVLHFLLSNCQYWLAEYRIDGFRFDGVTSMLY
                     YDHGLGKDFTNYNQYYDGNQDEDAIVYLSLANELIHSVNPNAITIAEEMSGMPGLAAP
                     LAAGGIGFDFRLAMGIPDYWIKIIKELPDEQWNVSQMYFELSNHRSDERAISYTESHD
                     QALVGDKTIIFRLADKEMYTHMSKFEPSLVIDRAIALHKMARLFTISTAGSGYLNFMG
                     NEFGHPEWIDFPREGNRWSYQYARRQWSLADNSTLKYEFLNDFDASMIDFISKAALHK
                     ASFPTLNHADEKNQVISFERNEFVFVFNFNPSQSFTDYNIPVHPGKYQIVFSTDDPEF
                     GGFNRIDKSYIFYATAGKEITTLKSNAVTLYIPSRTAFVLKMLPTKSVY"
     gene            10593..12239
                     /locus_tag="PK252_09035"
     CDS             10593..12239
                     /locus_tag="PK252_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT14889.1"
                     /translation="MKLRLTISAKIILGFGVLIIACLLNMWFFYNVLQKNQEQNKKFE
                     ELYDPSISNFEQLQNQVYAARLLIKNWVYIESETVTPDKLSLMDIMELKIPYLTDKLI
                     KLSPQWPRTDQIIYNDIQILLNDSLALLYTFVSKTFNSPEAYQLKNEKVLLKPDFNKT
                     LDRICDKINTKTTILLKNQQERSNSLKASLNATYTHTRSMILFSTIVLILIGLGISIY
                     TIHSLIGPINYIKKILFKMGKGILPAEKLRERNDEIGEMAVALNSLVRGLKEISNFSI
                     EIGKGNFNSNFTPLSDDDILGNSLIKMREELSNAALEEVKRKKEDEHRNWTTQGIAKF
                     SEILRQNSSDLQELSNNIISNLVNYLGANQGGIFIVNDSSLEGITIDMTGCYAYNRQK
                     FLKREFKPGEGLIGRCYLERETIFLTEIPKDYIKITSGMGEDNPTCLIVVPLLYNDKV
                     FGVIEIASFNIFEQFQIDFVEKIASSIASTISVVKINIQTNKLLEQSRQQAEEMASQE
                     EEMRQNMEELRATQEESARKEAALLKELKELKKRMEKYEL"
     gene            complement(12312..13610)
                     /locus_tag="PK252_09040"
     CDS             complement(12312..13610)
                     /locus_tag="PK252_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT14890.1"
                     /translation="MTVNTKTIGKYRYRILALIFLATTINYLDRSILGVLGPTLRDKV
                     FHWSNSDYALINVSFKVAYAFGLLFMGSIIDKVGVRLGYTISIAIWSIFGMMHALVQP
                     AFSLIGFITARFGLGFGESGNFPAAIKTVAEWFPKKDRAFATGIFNAGSNVGAILAPL
                     IIPLVVNSATGENWQFAFLVSGTFSAIWVFLWWRTYRLPQFHPKVTKAELDYIEADSG
                     IETPSQVKWSKLFRFKQTWAFSLGKLTDAAWWFYLFWATFFLTDKFHLQLSQLALPMI
                     VIYVMADIGSIAGGYISKYLMTMGWTLNKARKISLLICVLFILPVCFATQTNNPWIAI
                     ILIALAAAGHQAWSANLFTVVSDVFPKKAVASVVGIGGLVGALAGVVADLSLGKVLDD
                     SGPSAYFFAFLFAGLIYVFALIVIQILLPKLDAVNEKDIE"
     gene            complement(13683..14876)
                     /gene="uxuA"
                     /locus_tag="PK252_09045"
     CDS             complement(13683..14876)
                     /gene="uxuA"
                     /locus_tag="PK252_09045"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOT14891.1"
                     /translation="MMALEQTWRWYGPNDPITLQEIRQTGATGIVNALHQIPYGEVWP
                     VEEIMKRKKMIEDAGMRWSVVESVPVHEDIKKRTGSYQQRIENYKETLRNLSKCGIDT
                     VCYNFMPVLDWSRTSLDMKFPDGSYALEFKFSNFLAFELFILKRSGEEAKYTAEQIAE
                     AKKVYESMSNEDIENLKKVVMQGLPGSDDSFTLEQLHEALDQYKGISREKLKVHLILF
                     LKEVIPVAEEVGIRMAIHPDDPPRPVLGLPRVVSTLEDLKDIVSAVDSPANGITLCTG
                     SLGAGSFNNCPLIAKTLAHRINFTHLRNVKRDESGNFHEDYVFNGDVDLYEVMKTLVL
                     EEEKRKKEGRKDWQIPMRPDHGNLMLGDIGRKFYPGYSLYGRMKGLAELRGLEIGIRN
                     SILNK"
     gene            complement(14971..15795)
                     /locus_tag="PK252_09050"
     CDS             complement(14971..15795)
                     /locus_tag="PK252_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT14892.1"
                     /translation="MSYTEELFGIKDKVYVLTGGGGVLAGAIAEGIAKAGAKVALLDI
                     RIENAQKRAELINKNGGVAKAYETNVLDLSVLEKTRDEINKDFGDIDVLINLAGGNMA
                     GASIMPDQTIFDLKVPDFEKVVNLNLNGTVLPTIVFTKDMAKNKKGCVINISSMAAFR
                     AISRQLGYSAAKAAVSNFTRWMSMEMAIKFGDGIRVNALAPGFFIGDQNRALLINEDG
                     SYTDRGKTVIANTPMGRFGEADELIGAVIFLSSNASKFVTGVVLPIDGGFEAYSGV"
     gene            complement(15802..16476)
                     /locus_tag="PK252_09055"
     CDS             complement(15802..16476)
                     /locus_tag="PK252_09055"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOT14893.1"
                     /translation="MAKYTRIQVASAMQETGLVPVFYNANVEACKKVLKACYDGGARV
                     FEFTNRGDFAHEVFAELNKYALSELPGMILGVGSVVDGATASLYIQLGANFIVSPLLK
                     EDMAIACNRRKVLWAPGCGSLNEISRAEELGAEIVKIFPAKQVGGPDFVKAVKAPCPW
                     SSIMPTGGVEPEEENLRKWFDAGVVCVGMGSNLVSNDVVKNNSYEDLARKVKDVLNII
                     KKVRNK"
     gene            complement(16563..17597)
                     /locus_tag="PK252_09060"
     CDS             complement(16563..17597)
                     /locus_tag="PK252_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT14894.1"
                     /translation="MAKIVSFGEIMLRLSTPEHQRFTQTNSFVASFGGGEANVAVSLA
                     NFGLDAEFVTRLPQNDLGQAAVMDLRKYNVGTKNIVWGGDRIGIYFLETGAVARPSKV
                     LYDRAHSAIAEIEPGMINWEKVFDDTTWFHWTGITPAISEGAAKVCLEAIQVANKKGI
                     TVSCDLNYRKNLWKYGKKASEIMPELVKGCDVILGNEEDAEKVFGIKPEGVDVSKGHV
                     EGAAYESVGRQLMQKFPRAKKVIITLRGSVSADHNSWSGVLWDGNKLYEAPTYQITHI
                     VDRVGGGDSFMGGLIYGLITYKNDDQKALNFAVAASCLKHTIHGDYNQVTVSEVEQLM
                     KGDGSGRVVR"
     gene            17691..18707
                     /locus_tag="PK252_09065"
     CDS             17691..18707
                     /locus_tag="PK252_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT14895.1"
                     /translation="MNSKKITIHDIARKLNLTASTVSRALNNHPKISDATKKLVHNAA
                     KELNYRPNTIAASLRRGKAKSIGLVIPRINRNFISNTISGIEKVTSENGYNLLICQSE
                     ESLEKEINNLKTLVNNRVDGILISISLETNTSKHIKTIINDGIPLVQFDRFRSDIETS
                     KVINDNFQGAYDAVKHLIDKGCKRIAHFGGADIISIYAERIEGYKKSLIDNGLEVDPS
                     LIFNKILNKEEGYKIANKIFDSKIKIDGIFAASDYSALGAMLAARERNIKIPEQIAII
                     GFANEPFTEFVSPSLSSLDQFGVEMGISAARLLIEKIESKQKEVIPKTVIIRPKLIIR
                     ESSK"
     gene            18742..20139
                     /gene="uxaC"
                     /locus_tag="PK252_09070"
     CDS             18742..20139
                     /gene="uxaC"
                     /locus_tag="PK252_09070"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOT14896.1"
                     /translation="MKPFLDENFLLETKTAEVLYHEYASKMPIIDYHNHLPPDEIAHD
                     KQFETITQLWLGGDHYKWRAMRTFGIEEKYVTGDASDWEKFEKWAELIPYTLRNPIYH
                     WTHLELQRYFNISKQLSPATAKEIYNECNRLLQTPEFRVQKLLQKANVEVLCTTDDPI
                     DSLEYHKVIGNSQSFKVYPTWRPDKAMNAENTVTFNTYLNKLSEVSGIEISSYARLIE
                     ALEKRHDFFAQHGCRLSDHGLEEFYAEDYTLAEIETIFAKARNNKALTIEELAKYKSA
                     MLVDFAAMDARKGWAQQFHYGVLRNNNSRLFKKIGPDTGFDSIGDFTVGRNMQRFLNR
                     LDENSQLPKTILYNINPADNELIATMIGNFNDGSIKGKMQFGSAWWFLDQKDGMEKQI
                     NALSALGLLSCFVGMLTDSRSFVSFPRHEYFRRILCNILGNDIEKGMLPNDTAFIGKV
                     VQDICYNNAKKYFGF"
     gene            20302..20553
                     /gene="rpsT"
                     /locus_tag="PK252_09075"
     CDS             20302..20553
                     /gene="rpsT"
                     /locus_tag="PK252_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT14897.1"
                     /translation="MANHASSEKRIRQTETRNLENRYYAKTARNAVKKLRNTTNKEEA
                     AKYFPTVASLLDKLAKKNVIHNNKAANLKSSLAKHVNSL"
     gene            20680..21321
                     /locus_tag="PK252_09080"
     CDS             20680..21321
                     /locus_tag="PK252_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT14898.1"
                     /translation="MASIRVILADDHALFRNGLKLLLSGQSNIEVVGEASDGIELLKV
                     IEEIRADVVLLDIEMPMMNGIDAAYKALQIDPQLKIISLSMYSEEEYYYKMIEAGVKG
                     FILKNSDISEVVEAIETVYKGNTYFSSDVLYNVVKNIKTVGLKAEKAANISDRESEVL
                     EQICKGLSNQEIADNLFISRRTVEKHRASLLLKTNTKNTAQLIMYAIENKLLE"
     gene            21337..22596
                     /gene="lysA"
                     /locus_tag="PK252_09085"
     CDS             21337..22596
                     /gene="lysA"
                     /locus_tag="PK252_09085"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT14899.1"
                     /translation="MNTKKLPFSLEEIKEIIKTHPTPFHIYDEAAMRENARKLKAAFT
                     WNKGFKEYFAVKAAPNPVLMKIFKEEGFGADCSSLPELVLAEKVGIKGEDIMFSSNNT
                     PAKEFVKARELNAFINLDDIRHLPFLAKHAGIPETISFRYNPGSLKEGNLIIGHPEEA
                     KYGLTREQLFEAYKQAKEMGIKRFGLHTMVASNELDPAYFPETANILFALVKEITEKI
                     GITIEFVNLGGGIGIPYRPEQKAIEWKAFGDAIKAEYDKIIIGNGLPELKIFLESGRA
                     ITGPYGYLVTTVQHLKYTYKNYVGLDASMQNLMRPALYGAYHHITVMGKENQPHTVKY
                     DVTGSLCENNDKFAIDRMLPEIEEGDIVVIHDTGAHGHAMGFNYNAKLRSAELLLRPD
                     RSVMQIRRAETMDDYFATLDFKLIDKV"
     gene            22680..23240
                     /locus_tag="PK252_09090"
     CDS             22680..23240
                     /locus_tag="PK252_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14900.1"
                     /translation="MNRKIMPNLQQLLSEARSRFAIDEVVRFVAITEGQIPLLIDLML
                     DPKAEVNKKAAWAFSHLSDADPAYYKQYTERIVSALSKCNTSVERNLLRYLAIIDIAE
                     EQQGFLTEHCYKRLLNPLTPSANRVHALQILCNMAILYPELQEELCITIESLMSTENS
                     GSIQAHGKMVINQLKGKEKRKLTTMP"
     gene            complement(23294..24745)
                     /locus_tag="PK252_09095"
     CDS             complement(23294..24745)
                     /locus_tag="PK252_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT14901.1"
                     /translation="MALEKLSPKQVWQHFEELCNIPRPSKHEAQAGEYVIAFAQKHSL
                     YCEKDRVGNIVIRKPAAKGKESKPMITLQAHLDMVPQKNSDTVHDFTKDPIKPQIDGE
                     WVKASGTTLGADNGIGVAAALALLESDDVAHGPIEALFTIDEETGMTGAFGLEEGFLK
                     GSVLLNLDSEEFGEIIVGCAGGINTIATFNYFTETVSGDVKAYKLCLTGLKGGHSGVD
                     IHLGRANANKLLNRFMWNAEHFFGMRICSINGGNMRNAIPREAFAEVTIPSNFADDFI
                     KSVQNFQHIYQEEFASVETGLSFAAKEIPTPSTWIDKNTSEKLLDSVYAIPNGIIRMI
                     PEMPDVVETSTNLAIVKSDDANKTIQVVSLLRSSVDSAKKDLCNMVESIFDMAHACTV
                     HEGDYPGWKPDFKAPVLQTMKSVYLSMYGELPQIKVIHAGLECGIIGSLYPTMQMASF
                     GPTIKYPHSPDEKVNIASVENFWNYLVKIVEGV"
     gene            complement(24821..26719)
                     /locus_tag="PK252_09100"
     CDS             complement(24821..26719)
                     /locus_tag="PK252_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOT14902.1"
                     /translation="MKHVVFTLLLSCSFFSVFGQLTYSVDIIPDSLKANANAVVREFD
                     ISYERLNLSDYVKKVHIVTTILNEKGKNNAALVIYYDNNSDITEVNGNVFDRNGFITK
                     KVKRKEMRDYAAFENYIMFSDNRALTFLPAINMYPFTIEYNYTVKYKGAVGIETWEPQ
                     YSYSLSVQAASIRYKTPSNLELKYLPLNYNFDFTKNESNAEIEYGWKVKNLKAVKKEP
                     FMPTSIEVLPTLLLTPSRFSYERTEGDFSSWENFGKWIYTLNKGREELPVETMNKIKA
                     LTNPIADKKEKVKAVYRYMQDKTRYVCVSVGIGGLQTAKASEVDKLGYGDCKALSNYT
                     KALLNCIGIKSYYTVIGNGNCQIRFTDFPSGGQTNHIILCVPLETDTIWLECTSQKMP
                     FGFIGSDNSNRFALLITEEGGKLVRTPTYADSTNLSTANTKLKVNADKSADFQIKRTY
                     SNLAYEWIFPLLYQSSKEQKDYLLEYFADANTTINTFSFKDLSGVSANGVMEASGRLN
                     SFTSTSGNRVFINPAIVFRRNNFSEIKKSRKQPLFIEYGYAYNDTLNIAIPDNCIIES
                     KPADLSFKSSLGEYSIRYENVGQKINIIRRFYCISGTYSTNQLGEIVSFIEKIDNADN
                     DKIVLKNK"
     gene            complement(26721..28478)
                     /locus_tag="PK252_09105"
     CDS             complement(26721..28478)
                     /locus_tag="PK252_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14903.1"
                     /translation="MNGVPYYAEGKDEEKVTDIKAVSYNLENGKIVKSKLDKDNIIKE
                     KTSENWYKIKFALPNVKEGTIIEVKYSIISDYIFNLREWAFQSTIPILESAYKVWIPE
                     YFFYNQTQKGYFPITTEKDSKFTKLTLTYIQEDMSMSRKGHASSSNTFEYREDMFFYR
                     AKDVKAFPDDEYVKTIDNFLTKIEFELNHTKYPNQPIENYTSTWESVNEKLMDNEYFG
                     KALKSGGHLDDATAQLKQSGLTGISLVNAAFGMVKSKMAWNSINSKYVENSLRKAWKD
                     GAGNCADINLNLVALLRQLGFSSNPVILSTRNNGIIHPAHPSMSRFNYVIALVNLDGK
                     QYLLDATDPNAEINLLPVRCLNDKGRIVNEVKGDWINLMDYKPYILNSRYELTLDDAL
                     NLKGKALLQFKDYGAYSYRKDIKKVGEADYLKEMENEYKNTTLENLTINGLDSVNKLL
                     SINFDLTKKTNINKTDSLIFFSPAFFAYVENNPFKAETREYPVEMKYPYTIYQTNVIT
                     LPENYKVVELPKSYMAKLPDNKGKYTYMVNVNGNVINTSIIFQLNASLFLPEEYKNLR
                     SFYQTIVDKQNEMITVKVN"
     gene            complement(28441..28770)
                     /locus_tag="PK252_09110"
     CDS             complement(28441..28770)
                     /locus_tag="PK252_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14904.1"
                     /translation="MNSKLLKHLAFITLSLNTFFCFSQKEPKFGDINKEDIYNNVCPI
                     DSNANAYYIFDYGKSTFEYASTTIRVDEPASSRKGFQINFERHFRIKVINNNGLEWSS
                     ILCRRKR"
     gene            complement(28892..29605)
                     /locus_tag="PK252_09115"
     CDS             complement(28892..29605)
                     /locus_tag="PK252_09115"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOT14905.1"
                     /translation="MKLFDKIYNGFIKVFGDIKIYRFPMFILYDPGSYRVKGDDIREV
                     LDLVKPGDILLRGYVNYLDGYIIPGFFSHAGLYIGEVKPEDATTLSPEQMKNFKAGKQ
                     IVIHSMAEGVFMEDVLNFCRCDYMMILRRDAAKESAQAQSYTFEMVFTQALKDLGLEY
                     DFKFDFSDFHKLSCTELVYDACKGFIEEYGIKPKARTVMLMKKRLITPDDYANSPLTM
                     VWKSRSIGDKQMKKIKKAK"
     gene            complement(29891..>30838)
                     /locus_tag="PK252_09120"
     CDS             complement(29891..>30838)
                     /locus_tag="PK252_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14906.1"
                     /translation="AAGTGHILLNYTNSSGCTAATATDQAITINSLPTPLITGSATVC
                     AGSSGNVYTTDAGMSNYQWTITGGTITAGGTTSSNSVTVTWGAAGTGHVTLNYTNGSG
                     CTAATATDQSITINALPAITSNPVNSTIFAAENTSFSVTATGTAITYQWYENQGLGWN
                     SLTNGGVYSNVTTATMNITAATLSMNGYQYRCEVTGACPPAAVSDAALLTVNPTATVE
                     ISGSTTICAGETATLTLTLTGTANWSVSYNAGAVPQTIPSITTTTYTFNVSPAVTTVY
                     TLTGVTDANGAGTIVAPGAVTVTVNPKPSPGPLYRLPNQ"
BASE COUNT         9920 a         6292 c         5470 g         9156 t
ORIGIN      
        1 taatttgcta atttgctaat ttgctaattt gctaatttgc taatttgcta attatctttt
       61 aattactgca ttaattttgc caaaattatt actcccggct ttcactacaa ctgaaagtgc
      121 tttagtttta ggcttattag gctggataaa actcaaggta tcattgttca tcacagcttt
      181 aagcaggtag gttttcactt taacattgta aaaagccaca tttccctcgc tgtctgtaaa
      241 agccgacaag gtatctttgc cactgcaaag caatatttta gctgaagcta ttgcatacgt
      301 agcattggac gacttgtaca tcagctttcc ataaacaaca tcagcatttt gggctgatcc
      361 ataacttata aatatgaata acagtatgat tgaagttata taatttttca tctttcgtaa
      421 tttttaatta ttaattgaag atttactgaa ttgcaattaa ggatacccct gattttaagg
      481 agaaagtaaa tttagcggaa tcggcaacaa tcatagcagt atcgtacaga tataccgtgc
      541 ggggcagata acccggatat tccagcttaa tagcagggtt acttttttta ctaaacccat
      601 gacttgtaga tatattgtcc atgcggaagt ctccgttaaa atcggtagaa gtggttggcg
      661 atggaggctg tatgctgact ttaatgccat cgtttacatt ttttccaaca agcttaacat
      721 tgccaaccag tgtccaggta ttttcaacta cctgaggctg gggttggtca atagttgatg
      781 tagttttaag caagttgtta atcatcatat acgaagcgcc aaggacaatg ataaaacctg
      841 ctaaagcccc acctgcctgg tagtttttgt tatttattac agctgttgat tgtaaaaact
      901 taaacagtac aaaagccgcc accatgctca gtacaaatga caaggaaaac acgaccaaaa
      961 caaacatttt ggggtcggaa gtaacaatag ataattgagg gttcataatc tgatttattg
     1021 gtttgaacaa atatatcaaa acgctgaatg aaaacaacaa taggtcgtgc ataaattaaa
     1081 aaatactgcc atcaaaacaa attataccca ttcaggcaac atcccgaacc tatatttttc
     1141 gtaacttacc tgtaatttta cgcttcacat ttgcatgatc attattaata ttcaataaaa
     1201 ccatcttata atgaatagaa aaaggataat tcaagcattg gcaatggttg cgcttgtttt
     1261 ggccagttgc aacgtaaaaa aagaagtaaa gaacatccag aatggagttg ttgtaagcac
     1321 ttcagatgtt aacattaagg ttcagtttta tgcacccaac atggccaggg tacaaaaatg
     1381 gctgcctgcc ggaagcgaca aaaaacttag ccttgtgata gtgatggatt ctataccatc
     1441 tgttacggta aaagttacag aagagaaaaa cctggtaacc cttgaatctg aaagcctgaa
     1501 aattactatt aacgccaaag acggtgcagt tgcatttctt gatttgaaca acactccttt
     1561 tgtaaaagaa gctgattatt cttttaaacc agtggtttat tcagacgatt cggcctatac
     1621 cgtagaacaa aaatttgaac tcaccgctga tgaaggcatt tacggacttg gccaacacca
     1681 atcaggctat atgaactatc gtggcaaaaa agtattgctt tctcaagcaa acacagaggc
     1741 tgtaacccct gtatttgttt ctaccaggaa ctatggtatt ttgtgggaca actattcgct
     1801 taccgaatac cgcgacaatg gcaaggccga agcttctatg tggtctgaag tatctgacaa
     1861 cattgactat tactttattg caggaaacag ccttgacaat gtaattgccg gataccgcaa
     1921 ccttaccgga gttgccccaa tgtatggcaa gtgggcatac ggctactggc aaagtaaaga
     1981 acactatgat aaccaaaatg agataactac tgttgttaat aaataccgtg aactgaaata
     2041 ccctattgac aacatcattc aagactggga ctactggaat ggcactcaaa actggggaag
     2101 catgcagttt gacaaaacct tgttccccga accagaaaaa atggtgaaag attttcatgc
     2161 taaaaacttc cacatgatga tttctatttg gcctgccatg ggctccaata ccgaggtata
     2221 taaagacatg ctcgctaaag gctttttgta ttcgccagtt ggatgggcag gattcaaata
     2281 ctatgatgca tacaaccctg atgccaacaa gctttactgg gaatatctga aaaaaggcct
     2341 tgtatctaaa ggtattgacg catggtggat ggactcgacc gagccagatg tggtaaatgc
     2401 attgaccaaa gaagcagaaa actatgaaat gaaaaagatg ggcaagtgtt accttgggac
     2461 ttttgcccgc tatctcaatc cttactcttt ggccatgacc gaaaatattt ataaaaactg
     2521 gcgtaaagat gtacccggca aaagagctta cattctcacc cgctctacct ttgcaggaca
     2581 acaacgtaat gcagcaacca cctggagtgg agatattgga gctaactggc aggtttatcg
     2641 caaccagatt tcagcaggta tcaaccacag tatgtcaggt attccgtact ggacatttga
     2701 tataggcgca tttgtattag gtgcttacga aggcgtattt aatactggtg gtaaaaaccc
     2761 agcttaccaa gaactttaca cacgtatgtt ccaattaggc gctttttgtc ctatattccg
     2821 ttcgcacggt tctgaaactc ctcgcgaaat atgggaaatg ggggacttta cccctactct
     2881 tcttaaatat gataatcttc gctatcgctt gctcccatat atttactctt tggcctggaa
     2941 agtgaccaat gaaggttata ccatcatgcg tggtttgcct atggacttta gcaccgacaa
     3001 aaacacctat aatattgacg accagtttat gtttggcccg tcaatccttg tttcacctgt
     3061 aacagactat atgtatcatt gtcctcctca ggccagcatt aaagttggtg aagaattttt
     3121 caaaaccaaa gatggaaagc cgggtttaca agcaagctat tacaaagaca acaaatataa
     3181 aacgctgggc aaacaacagg ttgataaaaa tatagatatt ttctggtaca caggccgtcc
     3241 tgattatgta accgattcga tgtatgctat tcgttgggaa ggcaagctaa ttcctaaaga
     3301 aagcggcaaa ttccagttcc acatgaaatg ttttgacgca aaacgtatca ttctggatgg
     3361 caaacaactg ccaatcgttt ataccagcac cgaacaatat actgatactt tgcatctaca
     3421 agcaggcaaa gaatacagta ttgtagtaga aacagaaaac acatcaaccg gggctgcccg
     3481 tatgcaacta ttctggaaaa ccccgtctat atttgctgcc gaaaaagcaa aagttgaccg
     3541 cgagaaaaaa cgtacgcttt acctacccca ggctaaaggc tgggtaaact tctggactgg
     3601 cgaaactgcc aatggaggcc aaaagattac taccgatgcc cctatcgaca tcatgccttt
     3661 gtttgtcaaa gccggttcta tcgtcccaat gggcccgttt gtgcagtatg ccactgaaaa
     3721 acctgctgac ccgattgaac tacgcattta caaaggcgac aacggaagct ttgtattgta
     3781 tgaagacgaa aacgataact acaattacga aaaaggcatt tattcgacca ttcgtttcga
     3841 ctgggatgat gcttcaaaaa cccttaccat aggcaaaaga caaggtgtgt tccccggaat
     3901 gctcaaacat cgcacatttg agatagtact tgtttctgag aaacatggca ctggcctcga
     3961 tatcgaaacc aaacctgaca aggtaataaa atatagtgga gaagagctga aagtgaaatt
     4021 gtaaacaaac aggctttatt ctgagcaaca tagttgcgac atctttgtag agaaaagcaa
     4081 tacacagaaa cattaaggag cgtagcaccg aaataatatt tcgccactac gtggcttaat
     4141 aattgagaaa acatttttct acaaatattt cgccgctctg cggctaatag aaagacattg
     4201 caaatctgcc aaagtaaaac taaccagtcg gcataaaatt gtaatcaaac atgttcaata
     4261 aaaaaggagt acagcgaaaa tactgtactc cttttttata atccccaatt aatcattagg
     4321 aggcttttaa agctgaacga tatgattaat tctgtaaatt tgaccctaac ttattcttct
     4381 tcaaaacttt accaatattc agtttttaca taaaaaaagc cagtcgaaat aactggcttt
     4441 ttaaatatat caaaaaatat cttaatatcg gtaatgttct ggcttataag ggccatcaac
     4501 agggacacca atataatcag cctgagcttg cgtaagtttg gtaagtttaa cacccaattg
     4561 gcctaaatgc aaacgggcca cttcttcgtc cagcttctta ggcaaacgat acacacctat
     4621 ttcatactta tttgcccaca aatcaagttg ggcaagtgtt tggtttgaga atgaattact
     4681 catcacaaaa gaagggtgac ctgtagcaca gccaaggttt accaaacgcc cttcggccaa
     4741 aagaaaaata gaatgacctt caggaaaggt atatttgtcc acctgaggtt tgatattggt
     4801 atgcttaatc cctggcatgt taaccagttt gtccacctgt atttcgttat caaagtgacc
     4861 tatattgcaa acaatagcct ggtctttcat ctttttcatg tgctcaaggg taataacatc
     4921 acagttcccg gttgcagtaa cataaatatt cccctcagcc aaagcttctt ctatagtaga
     4981 tacttcaaaa ccttccatta cagcctgcaa agcattgatt gggtcaatct cggtaacaat
     5041 cacacgggct ccgtatgacc tcatagagcg ggcacaacct ttccctacat caccaaaacc
     5101 aaggacaaca accactttac cggcaatcat cacgtctgtg gcacgtttga taccatcggc
     5161 caatgattcg tggcaaccat aaaggttatc gaattttgat tttgttaccg agtcatttac
     5221 attgatagcc gggaataaca attcgccttt ttctaacatt tggtataaac ggtgtacccc
     5281 cgtagttgtt tcttccgaaa cgcctttaaa atcgcttaca atacgatgaa aacgggtttt
     5341 atcctcagcc aacgattttt tcaattgctc aaggataatt ctttcttcat tgtttgatgt
     5401 ttttctgtcc aaaacagaag catcattttc agctttgaag ccccaatgta caaacaaagt
     5461 agcatcacca ccatcgtcaa caataagatt aggccctaat ccattttcga agcgcaaggc
     5521 catatcggta caccaccaat attcttcaag agtttcgcct ttccaggcaa ataccggaat
     5581 tccggcagca gcaatagcag cagcagcatg atcttgtgta gaaaatatat tgcagcttac
     5641 ccaacgtact tcagccccaa ggtaaaccaa tgtttcgata agcacggctg tttgcactgt
     5701 catatgtaac gaacccataa tgcgggcgcc tttcaaaggt tttgatgaag catatttctg
     5761 acggatggcc atcaatccgg gcatttcctt ttcagcaatc tcaatttctt tacgtccaaa
     5821 atcagctaaa ctgatgtctt ttactttgta tttcaaagta ttgctcatga tatgtgtttt
     5881 aaaatattta tactatctgt tctatctttt tccaactgcc cgcgcaaatc gtcaatagag
     5941 gcaaatttgc gttcgtcacg caaacgggct acaaaggtaa ctgtaattat tgtatcatac
     6001 aaatcattat taaagtcaaa caaatgggct tcaatggttt tatgcaggct gttatggtca
     6061 accgtaggcc tgaaaccaat gtttaacatt ccattgaata tcccatagcc agtttttact
     6121 tttactgcat atactccaac tcttggaagc attttaaatt catcggtaac aatgttggct
     6181 gttgggaaag agagtgtcct tccaagcttt tggccttcaa caaccttccc cgtaataaaa
     6241 taattatacc ccagcaaatg gttggctttt tcaatctctc caagttcaag gtacaatctt
     6301 atgtctgttg agctcaccga atcacgtcca tcaaacaaag gggccgggtg aagcagctca
     6361 aatccatatt tcaaagaagc ctgacgaaca acttcgtaat taccttcttt gttatttcca
     6421 aaatggttat caaaacctaa aagtaagctt tgaacgccta ttttttcaac caaaactttt
     6481 tcaataaact cattggcttt caatgcagca aatcccttat caaaaggaac aagcacgagg
     6541 tgatcaaccc cactactttc caagagttct attttttcgt ccaaagtaga aagaagcttt
     6601 atttctttgc cgggataaag caccatacgc ggatgaggcc ataatgtaat gataacagtt
     6661 tgtcctgcat tcaataccgc ttgtctttgc atttgatcaa taatgtatcg gtgcccttta
     6721 tgaactccat caaaaatacc aacggtaata actggacgta taaattttga tttgtctaaa
     6781 tcagaaaaat aatgtgttgt caatgtgtca acctcccaag caatctttct tattgaagga
     6841 ttgaatacaa ttttaataat tttggtatat aataacagta aggacttgct tgttaaattt
     6901 tgcaaaataa ctgtctatag tcttgcaaag gttgttctta tcataagtat aaacgctttt
     6961 cgagaaatct tttttagcat cttcgcgggt ttgctcttct gatacattcc cggcagcatc
     7021 gtaagtatat tttgcaacca gaaactttat gccgtccccg ccttctttat acacttcact
     7081 cagcagccct cctttgtaaa cataagaaat cttgtaaata agtttccctt tcaggtattt
     7141 tgtctcagat aacatgttgt ccttactgtc ataggtatat tccaaacgtt ggctctcagt
     7201 attgtctggc attacagttg attcttgcaa aactttgtca ttgctgttga acaaatattt
     7261 ttgagtatat tttagcgatc cgtatccatc aaggattttc aaatccgaac tgttagagtt
     7321 gttagtaaat acacgttttt catcaaggcg tttttttata aaaaaattta cttcagccaa
     7381 tttaccatat ttgttataat tataaacatt tttgaaacta tctatcccgt tgtatccata
     7441 ctccaaagtt ttattgcctt ttgagtcaaa cctgacatcc tggataaaga cagtttttat
     7501 ccggccatca atatctgcct gtttagtttg aaaaaccctg ttgccttttg aatcgtattt
     7561 gcttgtagaa atactaagaa caagcccatt ctcacggtaa taggtctgca atacccgctg
     7621 cccatttgta tcaaagctgt caacacgggt tttaacccca tcagtctcag gtttcccgtt
     7681 tacatagtca tattcataaa aagtaacagt acggatttta tttttcttaa taatatcttt
     7741 gttccataaa ctgtcttgct ggccatagag gttaaatcca agagcaataa ctaaaaacag
     7801 caaaattttg cgctccatat tgtttagtat agtttctttt tttgaagatc aacaaaatta
     7861 atagagtttt tgtaatataa aaaattatct tttcatttga caaaggcaga atttatgggg
     7921 tagaatgctt gttttgttaa gaaaaagaga ataacttgca gtgaattgtt taaacaaaaa
     7981 aacttttgat aaacatatcc ggtaaaaaca tgtcacaaaa aacaccagta attgtccaaa
     8041 atgatacctg gcttgctcct tttagagata tcattgtcca cagaaatgaa ttggtcaata
     8101 aaaaaataaa aaaattggcc ccaaccggtt cattaaaaga ctttgcatta gggcatttat
     8161 tttatggatt gcacaaacaa aacgacgtat gggtttttcg tgaatgggcg cccaatgcta
     8221 ctgaaatata tcttgtcggt gatttttcaa agtggcttat cgacaagcaa tttgcactta
     8281 gaaagatagg aaatggcaat tggcagttag aggtgccatc tgactacata cgacacggac
     8341 aacaatacaa attacttgta aaatggagcg atggcgaagg ttatcggctc ccttcttatg
     8401 ccaacagggt agtgcaggac gaagcaacta aaatattcaa cgctcaagtt tggatgcccg
     8461 acaaagcata ccagtggaaa aacaacttta tcccttcctg ggatttcccg ttgatttatg
     8521 aggctcatgt cgggatgtcg tctgaagaac ctaaagtttc tactttcaga gagtttgaaa
     8581 acaatgttct cccccgcatt aaaaaacttg gttataatgc tcttcagctt atggccatac
     8641 aagaacatcc gtattatggc tcatttggat accatgtgtc aaactttttt gcagtttcat
     8701 caaggtttgg cactcctgat gacttaaaaa gcctgattga caaggcgcat gaaatgggca
     8761 ttgctgttat catggatctt gtacactctc atgctgtaaa gaatgtattg gaagggttgg
     8821 gcttgtttga tggcaaccct ggccagtact ttcataccaa cgacagacgt attcacaaag
     8881 cctgggactc gctctgtttt gattatggaa aaaatgaagt actacatttc ctcttatcta
     8941 attgccaata ttggctggcc gaatatcgta ttgacggatt taggtttgac ggggtaacca
     9001 gcatgttgta ttacgaccat ggacttggaa aagattttac taattataac caatattatg
     9061 atggcaacca ggacgaagat gctatcgttt acctcagttt agctaatgag ctgatacact
     9121 cggtcaaccc aaatgctatt accattgcag aagaaatgag cggaatgcct gggcttgctg
     9181 caccattagc cgccggagga ataggatttg actttaggct ggctatgggt attccggact
     9241 actggattaa aattatcaaa gaactgcctg acgaacagtg gaacgtgagc cagatgtact
     9301 tcgaattgtc aaaccaccgc tcagatgaac gtgccatatc ttataccgaa tcgcacgacc
     9361 aggctttggt aggcgacaaa accattattt tcaggcttgc cgataaagaa atgtacaccc
     9421 atatgagcaa atttgaacca agcctggtaa tagacagggc tattgcttta cacaagatgg
     9481 caagactttt taccatatct acggccggct caggatacct caactttatg ggcaatgaat
     9541 ttggacatcc cgagtggatt gactttccac gtgaaggcaa tagatggtct taccaatatg
     9601 cacgcagaca atggagcctt gcagacaata gcactttaaa gtatgaattt ctcaatgatt
     9661 ttgatgcttc tatgattgat tttatcagta aagctgcatt acataaagcc tcattcccaa
     9721 cgcttaacca cgcagacgag aaaaaccaag tcatttcttt tgagcgaaat gaatttgtct
     9781 ttgtttttaa tttcaatcct tctcaatcat ttacagacta taacatcccg gttcacccag
     9841 gcaagtacca aattgtattt tctacagatg accccgaatt tggcgggttc aacaggatag
     9901 acaaaagcta tatcttttat gctacagccg gaaaggagat tacaacacta aaatcaaatg
     9961 ctgtgacttt atatatccct tctcgtacag catttgtatt gaaaatgtta ccaacaaagt
    10021 ctgtgtacta aattagaaat aacatgaaac catttgacca tttcaaactt atttactttt
    10081 caacttgctc aaattggcct gcttgttgat ttttgttgtt aaaacagcaa agagcaggct
    10141 tttgtcagat tttttcagaa tactgtttta caaagaaaaa tttacttttt tcgacaatta
    10201 tgctgctaaa atcttaaaac gagcatgata gaaactgttt gatgttattt acaaattctt
    10261 aattaatact taatttgggt taaaaaatta tttaccgatg atacaaaata aaattccatg
    10321 atttatatca catgcaatgc cgtttagtta agagcgtccg caggacctgt cggcgttctt
    10381 ttaactaaac gacaacgata tcacatgtta catcgcccaa cattgatagt atcaaaaaac
    10441 tctaccctaa acagggctaa ataccaacag caacgtttca aattcgaaaa tgaaataaat
    10501 ttacaggtta gacaaaattt ctattaaatt tcatattttt actaattgta cagttatata
    10561 tttgagcgaa cataaccata aatatttgat gaatgaaatt acgtttgacc atatctgcta
    10621 aaatcatact tggatttgga gtgctgatta ttgcctgcct tctcaatatg tggtttttct
    10681 acaatgtttt acaaaaaaac caagagcaaa ataagaaatt tgaagaactc tacgacccat
    10741 caattagcaa ttttgaacag ttgcaaaatc aagtgtatgc cgctcgttta cttataaaaa
    10801 actgggttta tatagaaagc gaaaccgtca cgccagataa gcttagtttg atggatatta
    10861 tggaattgaa aatcccttat ttaacagata aattaataaa gctttcgcca cagtggcccc
    10921 gtaccgatca gattatttat aatgatatac aaatcttgct taatgacagc ttggcattgc
    10981 tatatacttt tgtttcaaag acctttaatt ccccagaagc gtaccagcta aagaacgaga
    11041 aagtattatt aaaaccagat ttcaacaaaa ctcttgaccg aatatgcgat aaaattaata
    11101 ccaaaacaac tattcttctt aaaaaccagc aagaacgctc caattcattg aaagcctcac
    11161 taaacgccac ttatacccat actcgcagca tgatcttgtt ttctactatt gtacttatcc
    11221 ttattggttt aggcattagt atttacacca ttcattcgtt gatagggcct ataaattata
    11281 tcaaaaagat tcttttcaaa atgggcaaag gtattttgcc agctgaaaaa ctcagggagc
    11341 gtaatgatga aattggagaa atggccgtag cattaaattc tctagtacga gggttgaaag
    11401 aaatatcaaa cttttctata gaaataggaa aaggaaactt caatagcaat tttacgcctc
    11461 tcagtgatga tgacattctg ggcaattcgt tgataaagat gcgcgaagaa cttagtaatg
    11521 ctgcattgga agaagtaaaa cgcaaaaaag aggatgaaca ccgcaattgg accactcagg
    11581 gcatcgccaa gttttctgaa atactgcgac aaaactcttc tgaccttcaa gaattgtcca
    11641 ataatattat cagtaacctg gtaaactatt taggggccaa tcaaggaggg attttcattg
    11701 ttaatgattc ttctcttgaa gggataacaa tagatatgac cggatgttat gcttataacc
    11761 gccaaaaatt tttaaaaaga gaattcaagc cgggtgaagg attaattgga cgttgctatt
    11821 tagagcgtga gacaattttt cttactgaaa ttcctaagga ttatataaaa atcacttcgg
    11881 gtatgggcga agacaacccg acttgtctta ttgttgtccc actattgtac aatgacaagg
    11941 tctttggggt tattgaaata gcatctttca acattttcga acaatttcaa atagactttg
    12001 ttgaaaaaat agcatcgagt atagcttcta ccatttctgt tgttaaaatc aacatacaaa
    12061 ccaataaact gcttgaacaa tcgcgccaac aagctgaaga aatggcttcg caagaggaag
    12121 aaatgcgaca aaacatggag gaattgaggg caacacaaga agagtctgcc cgtaaagaag
    12181 ctgcattgct caaagagctg aaagaactaa aaaaacgaat ggaaaaatat gaactgtaaa
    12241 aaagtaagat aagatgaaaa aatagaccat ctcagataaa aattgagacg atctatttaa
    12301 ttaatatcaa tttattcaat atctttttca tttacagcat caagctttgg cagtaaaatc
    12361 tgaattacga ttaaagcaaa cacataaatc aggccggcaa acaagaaagc aaagaaataa
    12421 gctgaagggc cgctatcatc caatacctta cctaatgaaa ggtctgccac tacgccagct
    12481 aaagctccta ccagcccacc aatgccaact actgaagcaa cggccttttt aggaaataca
    12541 tctgacacta cagtaaagag gttggccgac caggcttgat gccctgccgc agccaaagca
    12601 atcaggatga tagctatcca tggattattt gtttgggttg caaaacatac cggaagaata
    12661 aataatacgc aaattaacaa acttattttg cgcgctttgt taagggtcca acccattgtc
    12721 atcagatatt tcgaaatgta tccgcctgca atactgccaa tatcagccat aacataaata
    12781 actatcatag gaagagccaa ttgactgagc tgcaagtgaa acttatcagt aagaaagaat
    12841 gtagcccaaa aaagataaaa ccaccatgcg gcatctgtaa gttttcctaa agaaaacgcc
    12901 caagtctgtt tgaaccgaaa caatttagac catttcactt ggctgggagt ttctattccc
    12961 gaatctgctt caatatagtc caactcagcc ttggtaacct tcggatgaaa ctgaggtaag
    13021 cgataagtcc gccaccacaa aaacacccaa atagcactaa aagtaccaga tacaagaaat
    13081 gcaaactgcc agttttcgcc agtagcagaa tttacaacca gcgggataat caaaggagcc
    13141 aaaatagccc caacattgct ccctgcatta aagatacctg ttgcaaaagc cctgtctttt
    13201 ttggggaacc actctgccac agtcttgata gcagccggga agttaccgga ttctccaaaa
    13261 ccaaggccaa aacgggctgt aataaaacca ataagactaa aagccggttg taccaatgca
    13321 tgcatcatgc caaatatgct ccatatagca atagaaattg tataacctaa gcgaacgccc
    13381 accttatcga ttatacttcc cataaatagc aaaccaaaag cataagctac cttaaacgac
    13441 acattaatta gggcataatc agagtttgac cagtgaaaaa ccttatctct taaggttggg
    13501 cccaataccc ccaaaatact cctgtcaagg taattgatag tagtggctaa aaaaattaaa
    13561 gctaaaatac gataacggta tttcccaata gtctttgtat ttacagtcat tttagaccta
    13621 tttacagatt tataatttgt aaatataagt atttacaaca atctcacctt ctgatacaga
    13681 ctttatttat tcaaaataga atttctaatg cctatttcca atcccctcaa ttctgctaag
    13741 cctttcatac gaccataaag cgaatagcca ggataaaact tgcgtcctat atcacccagc
    13801 ataaggtttc catgatcggg acgcattggg atttgccaat ctttgcggcc ttctttttta
    13861 cgtttttctt cttccaatac cagtgttttc atcacttcat aaaggtcaac atcgccattg
    13921 aaaacataat cttcatgaaa attgccagat tcatcacgtt ttacattgcg caaatgagta
    13981 aagttaatgc gatgggctaa agttttagca ataagcgggc aattgttaaa gcttccggcc
    14041 cctaatgagc ccgtacataa ggtgatgcca ttagcaggag aatcaaccgc actgacaata
    14101 tcttttaaat cttccaacgt actaacaaca cgtggtaaac ccaatacagg gcgtggagga
    14161 tcgtcagggt ggattgccat gcgaattcct acctcctctg ccacaggaat cacttctttt
    14221 aagaaaagaa taagatgtac tttaagcttc tctcttgaaa tccctttata ttgatcaaga
    14281 gcttcatgta attgttcaag ggtaaaagag tcgtccgaac ctggaaggcc ttgcataaca
    14341 acctttttta gattttcaat atcttcattg ctcattgact catatacttt cttagcttct
    14401 gcaatttgct cagcagtata tttggcctct tcgcccgaac gttttaatat aaacagttca
    14461 aatgcaagaa aatttgaaaa tttaaattcc aaagcataag aaccatcggg aaatttcata
    14521 tccagacttg tccttgacca gtccaatacg ggcatgaaat tgtagcaaac agtgtcaata
    14581 ccacatttag aaaggttacg taaagtttct ttatagttct caatacgctg ctgataacta
    14641 ccagtgcgct tttttatatc ttcatgtaca gggacacttt ctacaactga ccaacgcatc
    14701 ccggcatctt caatcatttt cttgcgtttc atgatttctt ctaccggcca aacttcccca
    14761 taagggattt ggtgtaatgc attaacaatg ccagttgccc cagtttgtct tatttcttgc
    14821 aaggtaattg ggtcattagg cccataccaa cgccatgttt gttctaatgc cataatatta
    14881 atttgaaaat gtgagaatta gcaaattcga aaatgtaaaa aaattttatc tttattcatc
    14941 agctaattag caaattagat aattggctaa ttacactccg ctataggctt caaaaccacc
    15001 gtcaataggc aatactacgc ctgtaacgaa tttagaagca ttgcttgaga ggaatatcac
    15061 cgctccaatc aactcatctg cttcgccaaa acggcccata ggggtattgg ctataacagt
    15121 tttacctctg tcagtataac tgccgtcttc gttgattaac agagcacgat tttggtcacc
    15181 aataaagaat ccgggggcta aagcattaac acgtatgcca tcaccaaatt taatggccat
    15241 ttccatagac atccatcggg taaaattgct tactgctgcc ttagcggcag aatacccaag
    15301 ctgacggctg atagccctga aagcggccat agatgaaatg ttaataacgc accctttctt
    15361 gtttttagcc atatctttag taaaaacaat ggtaggcaat acagtaccat tgagattgag
    15421 gtttactacc ttctcaaaat caggcacctt aaggtcaaag atagtctggt caggcataat
    15481 gcttgccccg gccatatttc caccggcaag atttatcaaa acatcaatat cgccaaagtc
    15541 tttgtttatt tcatcacggg ttttttctaa aaccgacaaa tccaacacgt tggtttcata
    15601 ggcttttgct acaccgccat ttttattaat taattctgcc cttttctgag cattttcaat
    15661 acgtatatca agtaaagcaa ccttagcccc tgctttagca ataccttcag caattgctcc
    15721 agccaaaacg ccacctccac ctgttagtac atacacttta tcttttatcc caaaaagttc
    15781 ttctgtgtaa ctcatatttt tttatttatt tctaaccttt ttaataatat ttaaaacgtc
    15841 ttttaccttc ctggcaaggt cttcataact attatttttg acaacatcat tacttacaag
    15901 gtttgaaccc atgcctacac atacaacgcc cgcatcaaac catttgcgaa ggttttcttc
    15961 ctcaggctca acacctcctg taggcataat gcttgaccag ggacatggag ccttaacagc
    16021 cttaacaaaa tcagggcctc ccacttgttt tgcagggaaa atcttcacta tttcggcacc
    16081 aagctcttct gctcttgata tttcgttgag cgaaccgcag cccggagccc ataaaacttt
    16141 gcgacggttg caggcaatgg ccatatcttc cttcaatagc ggagatacaa tgaaatttgc
    16201 gcctaattgt atatacaaag aagctgttgc tccatcaaca acagaaccaa cgccaagtat
    16261 catgcctggc aattcactaa gcgcatattt attgagctct gcaaacacct catgggcaaa
    16321 atcgcccctg ttggtaaatt caaaaaccct tgcaccgcca tcatagcatg ctttcaatac
    16381 ttttttgcat gcttcaacgt ttgcgttata aaaaacaggt accagtcctg tttcttgcat
    16441 cgccgatgcc acttgtatcc tggtatattt tgccataatt aattaatttg aaaatgtgag
    16501 aattagctaa tttgagaata tttgtattca attgcaaatt aacaaattgg ctaatttgct
    16561 aattatctta ccacacgccc agagccatcg cctttcatca gttgctccac ttcgcttacg
    16621 gtaacctggt tgtaatcgcc atgaatagta tgttttaagc acgatgctgc tacagcaaag
    16681 tttaaagcct tctggtcatc atttttataa gtgataaggc cataaataag cccacccata
    16741 aaggagtctc cgccaccaac gcggtcaaca atatgtgtta tctggtatgt aggagcctcg
    16801 tatagcttat taccatccca taaaactcct gaccaggagt tgtggtctgc actgaccgaa
    16861 ccacggaggg taataatcac tttctttgcc cttgggaact tctgcatgag ctgacgacct
    16921 acagactcgt aagcagcgcc ttctacatgg cctttagaaa catcaacccc ttcaggctta
    16981 atgccaaaga ccttttcagc atcttcttcg ttgccaagga ttacatcaca accttttaca
    17041 agttccggca tgatttcaga agcttttttg ccgtatttcc aaaggttttt gcggtaattc
    17101 aaatcgcatg aaacagtaat acccttttta ttagctacct gaatagcctc aaggcatacc
    17161 tttgcagcgc cttccgaaat tgcaggcgtg ataccagtcc aatgaaacca ggtggtatcg
    17221 tcaaaaactt tttcccagtt gatcattcca ggttcaattt cagcaatagc tgaatgggcc
    17281 ctgtcatata aaactttgct tggacgagct acagcacccg tttcaagaaa ataaatacct
    17341 atcctgtctc ctccccatac aatattttta gtcccaacat tatactttct gagatccatc
    17401 acagcggctt gtcccaggtc attttgagga agacgggtaa caaactctgc atcaagccca
    17461 aaattagcaa gtgaaacagc tacattggcc tctcctccgc caaaagaagc aacaaacgaa
    17521 tttgtctggg taaaacgctg atgttcagga gtggacaacc tcagcatgat ttcaccaaaa
    17581 gaaacaatct tagccatagt atattataaa ttatttgatg caaagtaaaa taatttttca
    17641 atttaatgca atcgattgca tttatttttt atttttgtcc taataaaatt atgaacagca
    17701 aaaaaataac catacatgac atagccagga agctcaacct cacagcttct actgtgtcac
    17761 gtgcactaaa caaccatcct aaaataagcg atgccaccaa aaaactcgtt cataatgctg
    17821 ctaaagaatt gaactaccgc cctaacacca ttgcagccag tttgcgcagg ggtaaagcaa
    17881 aatctatcgg gttggtaata ccccgaatca atcgcaactt catatccaac actatttctg
    17941 gcattgagaa agtaaccagt gaaaatggct acaatttgct gatttgtcaa agtgaggagt
    18001 ctctggaaaa agaaataaac aaccttaaaa cccttgtcaa taacagagtt gacggaatac
    18061 tcatctcaat atcattagaa acaaacacat caaaacacat aaaaaccatc ataaacgatg
    18121 gcataccctt agtgcagttt gaccgctttc ggagcgatat cgaaaccagc aaagtgataa
    18181 atgacaattt tcagggagct tatgatgccg tgaaacatct gatagataaa ggatgcaaaa
    18241 gaattgcaca ttttggggga gctgacatca taagtattta tgctgaaaga attgaaggtt
    18301 ataagaaatc ccttatagac aatggccttg aggttgaccc aagccttatc ttcaataaaa
    18361 ttctcaataa agaagagggt tataaaattg ccaacaaaat atttgactca aaaatcaaaa
    18421 tagatggcat ctttgcagca agtgattatt ctgcattagg agcaatgctg gctgcacgcg
    18481 aacgtaatat aaaaatacct gagcaaattg ctattatagg ttttgccaat gaacctttta
    18541 ctgaatttgt aagcccttcg ctgtcttctc tagaccaatt tggtgtagaa atgggaattt
    18601 cagcagcccg actattgatt gaaaagattg aatcaaaaca aaaagaggta atccccaaaa
    18661 cggtcatcat caggccaaaa ctaattatca gggaatcaag caaataacaa ctacaatgca
    18721 tttcaaaatc attataatac aatgaaacct tttttagatg aaaattttct tttagaaaca
    18781 aaaacagccg aagtgctgta tcacgaatat gcatcaaaga tgcctattat agactatcac
    18841 aatcacttac ctccagacga aatagcacac gacaaacaat tcgagacgat aacccaactt
    18901 tggctcgggg gcgaccatta caagtggcga gcaatgcgca cctttggtat tgaagaaaaa
    18961 tacgttactg gcgatgcatc agactgggaa aaatttgaaa aatgggcaga attaatcccc
    19021 tatactttaa gaaacccgat ataccattgg actcacctcg agttacaacg ctatttcaac
    19081 attagcaaac aactcagtcc agctaccgcc aaagaaattt ataatgaatg caatcgattg
    19141 cttcaaactc cggaattcag ggtgcaaaag cttttgcaaa aagccaacgt ggaagtacta
    19201 tgtactactg acgaccccat tgatagcctg gaataccaca aagtcattgg caacagccag
    19261 agtttcaagg tttatcctac ttggcgcccg gacaaagcta tgaatgccga aaacacagta
    19321 acttttaata cttacctaaa caaattatct gaagtgtcag ggattgaaat atccagctat
    19381 gcaagattga ttgaagccct tgaaaaacga cacgatttct ttgcacaaca tggttgccgc
    19441 ttatctgacc acggactgga agaattttat gccgaagact ataccctggc cgagatagaa
    19501 actatttttg caaaagccag aaataacaag gctttaacta ttgaagaact ggcgaaatac
    19561 aaatcagcaa tgctcgttga ctttgctgcc atggatgcac gtaaaggatg ggcccaacaa
    19621 ttccactacg gggtactccg caacaacaat tcgcgactgt ttaaaaaaat tgggcctgac
    19681 acaggttttg attcaattgg cgattttaca gttggccgca acatgcaacg cttccttaac
    19741 cgacttgacg aaaacagcca actgcccaaa accatccttt acaacattaa cccggcagac
    19801 aacgaactaa ttgccaccat gattggcaat tttaatgacg gttccatcaa gggcaaaatg
    19861 caatttggct cagcatggtg gtttctcgac caaaaagatg gcatggaaaa gcaaatcaat
    19921 gcattgtctg ctttagggct gttgagttgc tttgttggca tgcttaccga ctcacggagc
    19981 tttgtatctt tccctcgcca cgaatatttc agaagaatac tctgcaatat tctgggcaat
    20041 gatattgaaa agggaatgtt accaaacgac accgccttta ttggcaaggt tgtacaagat
    20101 atttgttaca acaatgccaa gaaatacttt ggcttctgaa aatatattct gaaaacattt
    20161 gaaaacaatg caacacctca atgttgaata tttttctttt tcgaaaagaa atttttgcat
    20221 acagttcact tgtttttcaa aaaaaatata tctttgcagc ctcatttatt aaaaagagat
    20281 attttataat atttaaaaga aatggcaaat cacgcatcat ctgagaaacg tatcagacaa
    20341 actgaaacca gaaacctgga aaacagatat tatgctaaaa cagcacgtaa tgctgtgaaa
    20401 aagttaagaa atacaactaa caaggaagaa gctgcaaagt attttcctac tgtagcatcg
    20461 ttgcttgata agcttgccaa gaagaatgtt attcataaca acaaggccgc taaccttaag
    20521 agcagcttgg caaaacatgt aaacagtctg taagctggtt tctcttaaaa aagataaaca
    20581 aaaggccttc attgaaggcc ttttttgttt taacatagca attttttagt gcttataagt
    20641 caaaactttt acttttgtaa agtttatcaa acaaaataga tggcttcaat cagagttata
    20701 ttagccgatg atcatgccct ttttcgcaat gggttaaaat tactattgtc tgggcagtca
    20761 aatatcgaag tcgttggtga agcctcagat ggcattgagc tacttaaggt tatagaagaa
    20821 ataagagccg atgtagtgct tttagacatc gaaatgccaa tgatgaacgg cattgatgct
    20881 gcttataaag cgttgcagat agacccacag ctgaaaatta tctccctttc gatgtacagc
    20941 gaagaagagt attactacaa aatgatagaa gcaggagtaa aagggtttat ccttaaaaat
    21001 tccgatatta gtgaagttgt agaagctatc gaaacggttt acaagggaaa tacatacttt
    21061 tcctctgatg tactttacaa tgttgtgaaa aacattaaaa ccgttggctt gaaagcggaa
    21121 aaagctgcca atatctctga ccgcgaatca gaagtgttgg aacaaatttg caaaggcctt
    21181 tcaaaccagg aaatagccga caacctgttt atcagtcgcc gaacagtaga aaaacaccgg
    21241 gccagcttgt tactcaaaac aaacactaaa aacacggctc aactcattat gtatgccatt
    21301 gaaaataaac tacttgaata ataaattgtt acaataatga atacaaagaa actaccattc
    21361 tcgctggaag aaatcaaaga aattattaaa acacacccta ccccttttca tatttacgat
    21421 gaagccgcta tgcgtgaaaa tgccagaaaa ttgaaagctg catttacctg gaataagggg
    21481 tttaaagaat attttgcagt aaaagccgcg ccaaatcccg ttcttatgaa aatattcaaa
    21541 gaagaaggtt ttggtgcaga ctgtagctca ttgcccgaat tggtattggc tgaaaaagtt
    21601 ggtatcaaag gcgaagatat catgttcagc tcaaataaca cccctgccaa agaatttgta
    21661 aaagccagag aacttaatgc ttttataaac cttgatgaca tccgtcattt gccatttctg
    21721 gcaaaacatg ccggcattcc cgaaacaatc agcttcaggt ataaccctgg ttcattaaaa
    21781 gaagggaacc ttatcatagg tcatccggaa gaagccaaat acgggcttac ccgcgaacaa
    21841 ctttttgaag cctacaaaca ggctaaagaa atgggtatca aacgtttcgg attgcacacc
    21901 atggttgcct ctaatgaact tgaccctgca tattttcccg aaacagcgaa tatcttattt
    21961 gctttagtaa aagaaatcac agaaaaaata ggtattacca ttgaatttgt caatcttggc
    22021 ggcggcattg gtattccata tcgacccgag caaaaagcta ttgaatggaa agcctttgga
    22081 gatgctatta aagctgaata tgacaaaatc attattggca acggcttgcc agaattgaag
    22141 atattcctcg aatctggccg agctatcaca gggccttacg gatacctcgt taccacagtc
    22201 caacacctga aatatactta taaaaattat gttggacttg atgcaagtat gcaaaacctt
    22261 atgcgtcctg ctctttatgg ggcataccac catattaccg tgatgggtaa agaaaaccaa
    22321 cctcatactg taaaatatga tgtaaccggc tcgctctgcg aaaacaatga caagtttgcc
    22381 attgaccgta tgctgcctga gattgaagaa ggcgacatcg tggttatcca cgatacaggt
    22441 gctcacggcc atgctatggg attcaattac aatgcaaaac tgcgctcagc agagctgctg
    22501 ctaaggcctg accgctctgt aatgcagata cgcagggcag aaactatgga cgactacttt
    22561 gcaacactcg attttaaatt gattgacaaa gtttaaagtt taccacccct acccctcctt
    22621 taaaaggagg ggaaatacaa tttcttttga gctaaggaat aaaatgaaaa aaaatttgat
    22681 tgaatagaaa aataatgccc aacctccaac aactactttc cgaagcccgc tcccgttttg
    22741 ccattgacga agtagttcgt tttgttgcta ttaccgaagg ccaaataccg ctacttattg
    22801 acttgatgct tgacccaaaa gcagaagtaa acaaaaaggc tgcatgggct ttttcacatc
    22861 tttctgatgc cgaccctgct tattacaagc aatatactga acgtattgtt tcagcgctca
    22921 gcaagtgtaa tacttctgta gaaaggaacc tgctacgata cctggctatc atagatatag
    22981 ctgaggaaca acaagggttt ctgactgagc attgttacaa acgccttctt aacccgctta
    23041 ccccttctgc taacagggtt catgccttac aaatactctg taatatggcc atcttatacc
    23101 ccgaattgca ggaagaactt tgcattacta ttgagagcct gatgagcaca gaaaattccg
    23161 gatctattca ggcacacgga aagatggtaa ttaatcagtt gaaaggaaaa gagaaaagaa
    23221 aactaactac aatgccttaa gtacactcaa aacttcaaca ttaactgttt ttttgaaaac
    23281 atcacgttga agcttacacc ccttctacta ttttcaccaa ataattccaa aaattctcta
    23341 ccgaagcaat attcactttt tcgtccggtg agtgtggata tttgatagta gggccaaatg
    23401 atgccatttg catagttggg taaagactgc caataatgcc acattcaagg ccagcatgga
    23461 tgacttttat ctgagggagc tcgccataca ttgacagata tacagacttc attgtttgta
    23521 aaacaggggc tttaaaatct ggtttccagc cgggataatc accctcatgc acggtgcagg
    23581 cgtgagccat gtcaaaaatg ctttcaacca tattgcaaag gtcttttttg gccgaatcaa
    23641 cagagctgcg aagtaaactg actacctgaa tagttttatt agcatcatca gatttaacaa
    23701 tagccaggtt ggtacttgtt tctacaacat caggcatttc gggtatcata cgaatgatac
    23761 cattgggaat agcatatacc gagtcgagaa gcttctcact ggtatttttg tcaatccatg
    23821 tagaaggtgt aggtatttct ttggcagcga atgaaaggcc tgtttcgaca cttgcaaact
    23881 cttcctgata gatatgttga aaattttgca cactcttaat aaaatcatca gcaaaattag
    23941 acggaatagt aacttcagca aatgcttcac gaggaatggc gttacgcata ttacccccat
    24001 tgatagaaca aatacgcata ccaaagaaat gctcagcatt ccacataaaa cggtttagca
    24061 acttattggc attggcgcgt ccaaggtgga tatcaacgcc cgaatgacca ccttttaaac
    24121 ctgtgagaca aagtttgtaa gccttaacat cgcccgagac cgtttcggta aaatagttaa
    24181 atgtagcaat ggtattaata ccaccggcac agccaacaat aatttcgcca aactcttcag
    24241 aatcaaggtt aagcaaaaca ctacctttca aaaaaccttc ttcgaggcca aaagcaccag
    24301 tcataccagt ttcttcatca atggtaaaca atgcttcaat tggcccatgg gctacatcat
    24361 cagactcgag caaagctaaa gcagcagcaa cccctatacc attgtcagcg ccaagggttg
    24421 ttccggaagc ttttacccat tccccatcaa tttgaggttt aatagggtct ttagtaaaat
    24481 catgaacagt atcagagttt ttctgcggca ccatatccaa atgtgcctgc aatgtaatca
    24541 ttggcttcga ctctttccct ttagcagcag gttttctgat aacaatgtta cccacacggt
    24601 ctttttcgca ataaaggcta tgcttttggg caaaagctat aacatattca cctgcctggg
    24661 cttcatgttt tgaagggcgt gggatattgc acaattcctc gaaatgttgc catacctgct
    24721 tgggagaaag tttttcaaga gccatatact ggtttttttg ccaaatttat ataaaaatgc
    24781 aattcggaca cattaatttc aacaaggatt atcatcctgc tcacttattt tttaatacta
    24841 ttttatcgtt gtcggcattg tctatttttt caataaaaga aacaatttca ccaagttgat
    24901 ttgtgctgta tgtacctgaa atacaataaa accgtctgat aatatttatt ttttgtccga
    24961 cattttcata tctgatcgaa tattctccta atgaagactt aaaagaaagg tcggctggtt
    25021 tgctttcgat aatgcaattg tcaggaatgg caatgtttaa agtatcgttg tatgcatagc
    25081 catattcaat aaacagaggt tgtttccttg acttctttat ttcagaaaaa ttatttcttc
    25141 taaaaacaat tgcagggttt ataaacactc tattaccgga tgtactggtg aaactattca
    25201 gtcttccaga ggcttccatg actccattcg cagaaactcc agacaaatct ttaaatgaga
    25261 aggtattgat agtagtattg gcatcagcaa aatattccaa tagatagtct ttttgctctt
    25321 tactggattg atagagcaat gggaatatcc attcgtaagc aaggtttgaa tatgttcgct
    25381 taatctgaaa atcagcactt ttatcagcat tgactttcaa ttttgtatta gctgtactaa
    25441 gattagtgga atcagcatac gttggagtac gaacaagctt tccgccttct tcagtaatta
    25501 aaagagcgaa cctgtttgaa ttatcactgc caatgaagcc aaaaggcatt ttttgactgg
    25561 tacactccag ccaaatagta tcagtttcaa gcggaacaca cagaataata tggtttgttt
    25621 gtcctccact aggaaaatca gtaaaacgaa tttgacaatt tccgtttccg attactgtat
    25681 agtaggattt gatgccgatg caattcaata aagctttagt atagttcgaa agtgctttgc
    25741 aatcgccata cccaagctta tcaacctcag aagcttttgc agtttgaagt cctccaatac
    25801 ccactgacac acatacatag cgggttttgt cctgcatgta tctatatacg gcttttactt
    25861 tctccttttt atcagcaatt gggtttgtta aagctttaat tttattcatg gtttcaacag
    25921 gcagttcttc tcttcctttg ttaagcgtgt atatccattt tccaaaattc tcccagctgg
    25981 aaaagtcgcc ttcagttctt tcataagaaa agcgcgatgg tgttaaaagc aatgtcggaa
    26041 gaacttcgat agaagttggc ataaaaggtt cttttttaac cgcttttaga tttttcactt
    26101 tccagccata ttctatctcg gcatttgatt catttttagt aaaatcaaag ttgtaattca
    26161 atggcaaata tttcagttca agatttgaag gcgttttata acgtattgaa gctgcctgaa
    26221 cagataaact gtagctatac tgaggttccc aggtctcaat accaaccgcg cctttgtatt
    26281 taacagtata gttatattca atagtaaatg gatacatgtt tatggcaggt aaaaaagtca
    26341 atgcccggtt atccgagaac ataatataat tctcaaatgc agcataatcc cgcatttctt
    26401 ttctttttac cttttttgtt ataaagccat ttctatcgaa aacattgcca ttgacctcag
    26461 ttatgtcaga gttgttatca taataaataa ccaacgcggc attatttttc cctttttcat
    26521 taagaatggt agtaacaata tgcacttttt tgacataatc actcaggttt aacctttcgt
    26581 agcttatatc aaattctcta acaaccgcat tggcattagc ttttaacgaa tcaggaataa
    26641 tgtcaacact ataagtcagt tgcccaaaaa ccgagaaaaa gctacatgat agaagtagtg
    26701 taaaaacaac atgcttcatc ttagtttact ttcacggtta tcatttcgtt ttgcttatct
    26761 acaatggttt gataaaatga tctcaaattt ttatactctt caggcaaaaa caaacttgca
    26821 ttcaactgaa aaataataga tgtgtttata acattaccat tcacgttaac catataagta
    26881 tattttcctt tattatcagg caatttggcc atgtatgatt ttggcaattc caccaccttg
    26941 tagttttcgg gcaacgtgat aacattggtc tggtaaatgg tgtagggata tttcatttca
    27001 actggatatt cgcgggtttc agctttaaaa gggttatttt caacataagc aaaaaaagcc
    27061 ggagaaaaga aaatcaagct atctgtcttg ttgatattgg tctttttggt caggtcaaaa
    27121 ttgatagaaa gaagcttatt tacagaatca agaccgttta ttgtaaggtt ttcaagggtt
    27181 gtatttttat attcattttc catttccttc aaatagtctg cctctccaac ttttttgata
    27241 tcttttctgt aagaatatgc cccatagtct ttaaattgca acaaggcttt accctttagg
    27301 tttagcgcat catctaaggt taattcatac cttgaattta aaatatatgg tttgtagtcc
    27361 atcaggttaa tccaatcgcc tttgacttca ttgacgatac ggcctttatc attaaggcat
    27421 cttacaggca ataggtttat ttcggcattg gggtctgttg catctaacaa atactgctta
    27481 ccatcgaggt taacaagggc tattacataa ttaaaccgcg acattgaagg atgtgcagga
    27541 tgaatgatgc cgttattcct agtactcaga attacagggt ttgaactaaa gcctaattgc
    27601 cttaacaaag caacaaggtt gaggttgata tcagcacaat tgccggcacc atctttccag
    27661 gctttgcgca aactattttc tacgtacttt gaattgatgc tattccatgc cattttactt
    27721 ttcaccattc caaaagctgc attaaccaat gaaatgcctg tcaagcctga ttgtttaagc
    27781 tgtgcagttg catcatccaa atgtccaccg ctttttaatg ctttaccaaa atattcgtta
    27841 tccattagtt tttcattcac actttcccag gtactagtat aattttcaat aggctgatta
    27901 ggatacttgg tgtggttaag ttcaaattct atcttagtaa gaaaattatc tatagttttt
    27961 acatactcat catcaggaaa agctttaaca tcttttgcgc gataaaagaa catatcttcc
    28021 ctatactcaa atgtattgct tgaggatgca tgtccttttc tactcatcga catatcttct
    28081 tgaatatacg ttaaagtaag ttttgtaaat ttagaatctt tttcagtagt aatagggaag
    28141 taacctttct gtgtctgatt ataaaaaaaa tattccggaa tccaaacttt ataggcactc
    28201 tccaagattg gaatagtgct ttgaaaagcc cattcgcgca agttaaaaat gtaatcggaa
    28261 ataatggaat attttacttc gatgattgtt ccttctttta cattgggcaa ggcaaatttg
    28321 attttatacc agttttcgct ggttttttcc tttataatat tgtctttatc taatttagat
    28381 ttcactattt tgccattctc caagttatag cttaccgctt taatatctgt aactttttct
    28441 tcatcttttc cttctgcata atatggaact ccattcaagg ccattgttat tgattacttt
    28501 aatcctaaaa tgtctttcaa aatttatctg aaaccctttt cttgagcttg caggttcatc
    28561 aacacgaatg gtcgtcgaag catactcaaa ggttgatttt ccataatcga aaatataata
    28621 agcatttgca tttgaatcaa ttggacaaac attgttataa atgtcttctt tatttatatc
    28681 tccaaatttt ggctctttct gtgaaaaaca aaaaaatgta ttaagagata aggttataaa
    28741 ggccaggtgt tttagaagtt ttgagttcat aaaataatgg attattggtt atgttagcaa
    28801 tattactctt tttatttttt aattggttgt attaacaata aaaaaacgca agacaaacag
    28861 ttatcctgag tttttttatc ttactaaaag cttatttagc ctttttaatc tttttcattt
    28921 gcttatctcc aatggaacgg cttttccaaa ccatcgtcaa tggtgagttt gcataatcat
    28981 ctggtgtgat tagccttttt ttcatgagca ttacagttcg ggctttgggt ttaatgccat
    29041 attcctcaat aaaacctttg catgcatcgt aaaccagctc agtacaggac aacttgtgaa
    29101 aatctgaaaa gtcgaatttg aaatcgtatt ctaaccccaa atctttcaaa gcctgggtaa
    29161 aaaccatctc aaaagtgtat gattgagcct gagccgattc ttttgctgca tcacgcctga
    29221 gaatcatcat atagtcgcaa cgacagaaat ttagcacatc ttccataaaa accccttcgg
    29281 ccatagagtg gatgacaatc tgcttgcctg ctttgaagtt tttcatctgc tcagggctta
    29341 acgtggttgc atcttcgggc ttgacttcgc caatatataa gccagcatgg ctaaagaacc
    29401 cggggataat atatccgtca agataattaa catatccacg gaggagaata tctccaggct
    29461 ttactaaatc gagtacttca cgaatatcat cgccttttac cctgtaactc cccggatcgt
    29521 agagaataaa cattgggaat ctgtatattt ttatatctcc aaatacttta ataaatccat
    29581 tgtagatttt atcgaacaat ttcatggttt ataattatta aaaggttata aaagttagat
    29641 tatctgtagt taaaagtaat aaaaatgagc aaaaacaaac attataaaca taaaaaagaa
    29701 gaacaagatt aaacttaaat taaataattt aatgtcctgt ttttatcggg agggtaaacc
    29761 caattaaata tcatatactt tggcaaaata ttgtttccta ataataattt gtgtaaaata
    29821 ctcccaattc gtaagataat ttctattcat tgaaaaaaaa ggataacctt tcggctatcc
    29881 ttttcatatc ttattgattt ggcaatcggt acaatggtcc gggagatggc tttggattga
    29941 ccgtgaccgt aacagcaccc ggagcaacaa tagtccctgc cccatttgca tcagtaaccc
    30001 cggtaagagt atatactgta gttacagcag gactaacatt aaaagtatat gtagttgtag
    30061 taatacttgg gatagtctga ggaacagctc ctgcattata actaacactc cagtttgcag
    30121 ttcctgtcaa agtaagggtc aatgtagccg tttcgccggc acaaatggta gtcgatccac
    30181 ttatttctac agtagcagta ggattaacag tcaataaagc tgcatccgaa actgcagcag
    30241 gaggacacgc accagtaacc tcacatcgat attgataccc attcatgctc aatgttgcag
    30301 cagtaatatt cattgtagcg gttgttacat tactatatac ccccccatta gtaagagaat
    30361 tccacccaag gccttgattt tcataccact gataagtaat agcagtacct gtagctgtaa
    30421 cactaaaaga tgtattttct gcagcaaata ttgtactatt aacagggtta gaagtaatag
    30481 caggcaaggc attaatagta atactctggt cagttgccgt agcagcagta catccactac
    30541 cattggtata gttcaatgtt acatggcctg tgcctgctgc tccccaggtt acagttacac
    30601 tattagatga ggttgtacct ccggctgtaa ttgtacctcc tgttatcgtc cactggtaat
    30661 tagacatccc tgcatcagtt gtataaacat taccggacga accagcacat actgtagcac
    30721 tacctgtaat taaaggcgtt ggcaaagaat taatagtaat agcctgatcg gtagcagtag
    30781 ctgctgtaca tccactactg ttggtataat ttaataaaat atgacccgtt ccggcagc
//
LOCUS       DAOSPF010000041        30481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1522_length_30481_cov_6.270394, whole genome shotgun sequence.
ACCESSION   DAOSPF010000041 DAOSPF010000000
VERSION     DAOSPF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_30481_cov_6.270394"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="PK252_09125"
     CDS             <1..1960
                     /locus_tag="PK252_09125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT14907.1"
                     /translation="IALIILGLINYQHTKTILIESNKSKNKIIADKIKNILEFQDMTF
                     SILDENMAQRMNGYSNAIVNRYAGNSKILESADLNALRSEIGMNPLMEDIYVINKEGV
                     IVNTTFKKDKGLDLFSFGEAHKKFLLSVFDGGRFVNERFAIEATTKRLKKYTYQPTRD
                     KKYIIEIGSYSKDADSIINLVINTLGKSVGLFIMTDKPFSITDAEVSEKHKHLLDKVF
                     VEKGQFTVFEEENDKKIEYTFINLNSKPSGLYKGSAIQIVTDRTEEINMLRRELIKLI
                     CIFGFAIILVVILIYRKTKIITYPIKKLVLNINRITHGHLDERAEVVGNNEIATLSKQ
                     FNKMIEELESYYNELEQKVKDRTAEIMQQKEEIEAQRDALEQQRNLLAQTNKSLEHAY
                     VEISEQKKHITDSIVYAKRIQNAILPPDEVIHKYLPDSFILYRPKDIVSGDFYWLSEK
                     EGLVMIAAVDCTGHGVPGAFMSIVGHNNLNFAVNVRGARKAGDILNELNQAVTDTLRQ
                     QHGNASIRDGMDIALCCFDFESRRLNFAGANNPLIIVRNGEAIKYDATKAPIGAYIGD
                     KLTNFTNNEIELSAGDMCYIFSDGYADQFGGPENKKFLKRKLIELLAEISTEPVNKQK
                     EMLEQAHELWKGEHEQVDDILIIGIRCK"
     gene            1972..3345
                     /locus_tag="PK252_09130"
     CDS             1972..3345
                     /locus_tag="PK252_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14908.1"
                     /translation="MTNPESNILIITSAKEGVAYDWALMLSESLGSAIAQLAGFSPAI
                     NVDSLDNVDSENFLKSKSAVYIVLDNMYISNQLQIEKVRRYTKIDNLNLFFILPAPQN
                     EKELKNITDKKITYYCFDEDPETGIERRYSAFEPPDISKLFWSKVLDIAYDFLQKKGK
                     SEVQIKKAVYLAITGPELFEYRDAIRSELIHRGYTVFPEVNLTNDTEKIAEEVSAYLS
                     KSYISIHLIGSKYGHIIPGIESSLPELQCRLSAKHLRLQAERLQHQTFQRLVWIQPKI
                     KPIEEKQKRFLNALRVEEKNFNTEILQISLEDLKVVLREKLDYIDGKIKSTEQNLNSV
                     YIIYEPKDYVKVLEIVQYFEKQNVKTLLIDFVKMADTIVASHYNYLTACSSVIIYDSG
                     NNPQWLSSKMKDLVKAPGFGRKNPFKVKAILTKNINGFSGTDNLLLDANKIDPALSIF
                     VQKLNQN"
     gene            3345..6437
                     /locus_tag="PK252_09135"
     CDS             3345..6437
                     /locus_tag="PK252_09135"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14909.1"
                     /translation="MDEYVKTANLEAISEIKAVINPFPGLRPFGIEESHLFFGREGQS
                     EEVLLKLSKNRFVAVIGASGTGKSSLIYCGLVPILYGGFIANAGSQWKIIATRPGGGP
                     IANMAESVVKSVYKDEADKEKQMKASFFDAMLRRSSLGLLEVLRNLPIAKDENILMLV
                     DQFEELFRYRLSRKGQEAVNESEAFVKLLVEAVNQTEIPVYIVITMRSDFIGECSNYQ
                     ELTTLINRSNYLIPQMTRNDFRDAIVGPVAVGNAKIETALVQELLNDVGDNPDQLPIL
                     QHAMMRTWDYWLNNSDRDKEVGLSEYEAIGKMEKALSEHANEAFDELSEEGQRICEVL
                     FKTITEKGADNRGVRRPTRLEDIADIAAADIDELKTVIEKFRAPGRSFVTPSSEVPLT
                     KDSIIDLSHESLMRIWNRLKVWVDEEASAVQMYLRLSEASALFQEGKTGLWRPPDLQI
                     ALNWEKKQKPTLAWAQRHNPAFERAMVFLHTSAKEYEADELNKIKLQKRALQRSRMIA
                     AILGVAAVISLGIMIYAQMQSTEAFKQRMAAIKSSVEANKQKSIALKNAQEANEQKKI
                     AEQQSIEANKQKEIALEQTQIALQNATEANRQKNIADAKSKEANEQKVLAEKSAKEAL
                     EQKLAAEIAREEAYNRRMLSIAQSMAVKSQQIDDDKDLKALLAFQAFTFNEKYQGSEK
                     NPDVFAGLYQAMITLNGKSQNIYKGHSDAINSIVFWPSASLFYTASSDGKVLKWDVSD
                     STKKPFVVIDNKKVVNKSIDISSDGKYLAVGTLGNGLGILDLKTKDASVRYAVVPYKN
                     IELVAFTNDAKTIVAASDSSLIEFDATTGAGTVVGKADGKILSLAVAPDGKTIATGTK
                     SGKVYLWDKVSSYKKELLFEEASNQIHSLAFNHNGSLLAAGNLIGYLKLWNLKEKKML
                     FNVKGHSARITDIKFSPNDKTIATASYDQNVLLWDASDLNIQPIKLKEHESWVLSLAF
                     NPFGDKLVTGSIKEDRLILWPTNTYDMASVVHKKMGRNFTIEEWNTYVGKDIRYERSK
                     "
     gene            6461..7489
                     /locus_tag="PK252_09140"
     CDS             6461..7489
                     /locus_tag="PK252_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT14910.1"
                     /translation="MIKVKLWIFLLSIACLLPFKVLGQEDCAFTLSKAQKLYDAGTIE
                     QIPEMLKMCMESGFTKEEKQQAMKLIIMAYLFDNNQKEADKEMLKFLKEYPEYEIVVS
                     DQAEFVQLFNTYRTLPIASIGLVGFTNMSYVTPISYYGTDGNKGSYKNTGFGFQVGLS
                     YLQYLMEYIDLNVEGFYVSSTYQFASSDTAKQITFEETQSKLEFPISAVFKPRSFGMF
                     VPYCRLGFGTSLLLSSNGSETTINPASMVKPNSNPSFDLMNRRKKFQIYGIIGAGVRI
                     NLSHSFLLLDVRYNQGFTQQFKKSARTSGDYINYVINTQHNENDFRINNLWMSIGFYY
                     KFYKPQKR"
     gene            7495..8700
                     /locus_tag="PK252_09145"
     CDS             7495..8700
                     /locus_tag="PK252_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14911.1"
                     /translation="MNKYIYYIILVLLAFVSCKEADMASDQEKSHIKFYGGSYQAEGN
                     DIKQLSDGCFVAFGTISFSEISKSMYLVKTDRLGNEIWSKVITDTISKTGVSFEVMPD
                     GGFVMLGNTITSPSRISVARTDANGVTLWTKYISRGKGIKGYSIKRTSDNGLVIAGSY
                     TNANDDLDAMLFFVDFAGNNEKLITAAWDENDEAKDVIELGNGIFIMAGYRTNISTKG
                     SNACFWIVNSKDEDFPFLHGLVIEKNKVSSIERVKFIGNSNYLMCGQVNTLDSAMNTF
                     VAKIKVFSPDNTTDYTIKPVWKRSYGSKNKDFATSCEMLSDNDFAILGTTYNDLGQTS
                     MTLYKIDSLGNETSAKKEYGGTSGSSVSQSGNCILKTQDGGLVTTGVNSYSKTTLLSI
                     IKFDSNGNF"
     gene            8867..16105
                     /locus_tag="PK252_09150"
     CDS             8867..16105
                     /locus_tag="PK252_09150"
                     /inference="COORDINATES: protein motif:HMM:NF040192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT14912.1"
                     /translation="MKRTIRFMFFFFLLLMTPVLLFAEGPTLSSVSIASNNSVLTQAK
                     VGDKIMLTFTGSEPLTSKPTVTILGHIIANENVVNTSGNTWTAEYTTLNSDTEGTVPF
                     TINYTDIAGNAGIAVTTVTDGSSVTFDRTLPTLSNVNIASNNSVLTQAKVGDKIMLTF
                     TGSEPLTSKPTVTILGHIIANENVVNTSGNTWTAEYTTLNSDTEGTVPFTINYTDIAG
                     NAGIAVTTDGSSVTFDRTAPTVIISSSAANPTNVSPIPLTITFSEDVTGFVVEDITTS
                     GGSLSGFSGSGKVYTVNYFPVYGTNNINIAANKVIDIASNGNIAASQFSIEYYKTPEV
                     EFVSGDTICANETVVNLSATAKDNRGTIYWYTEVTGGTHVASGNNYSPNITTTTSYYV
                     EVNDHGFISSPRIKVDAIVYPLPLTTATNTGPVCEGSAISLVGGPITPDIVNYSWTST
                     PAGYTNSYHSPLLSSNATEGLSKTYTLKTTDIHGCVNTASTVVTVNATPTIYNVTGGG
                     AYCAGGPGMSIGLDGADKNTTYTLKRGITPVATYNSGTNTSAFTFGTFTQAGEYTVVA
                     TSSCTANMNGSVNITVNSLPTVYAFNNSPICAENSLVLNALPSGLNSYSWTGPAISGS
                     LSGQSITLTNAGAVSHSGTYTLTVTDDNGCVNTATTVAKVNALPSPSIVGLDSAYKTT
                     DSNIELVGDPSGGTFSGLGVISSINKFFPNLVSKIGESFDIKYTYTDEKNCTKVAKQS
                     VVVLKTSAIFESINPNSTYCYTDTSDEIWAKSPDIVGNGYYKHSKKHGLQDLGNNHAI
                     LTPSLMGAGNDTITFFFKTVAGDDKISRVVFIDSVGTISINTPADEFCENDNQTVTLK
                     TVNAGTNGTKNWIWPGTVVNGDYTFVPSSLTKNTSYNFSVTYVSTNGCSSNTADTDIF
                     INEKPNDTIYNEKTFNVYNEAEELKGGKVFPLYSFSAPKGITYDGSVYKFNPAVVPAN
                     TSVVVTYSYTDNKSCTNTATKTFNIYSPVGTITNLGSAKDTFCYGEAIAHYKFTTTSP
                     YSALLFYIDNKDYTSQLSGNEVTFPIGSDIGQGLHTARFLYKNGHSWFEVSKTFYVEK
                     AVAGTFVINYANGKDYACQADNNVIITVNGYAPVGPASSFSWTNALNTTISRAEISTQ
                     NAGSIPFSYVYISPFGCHSDPIAGSVTIKALPQDKLGVGTIYNLADGAKPLKGSGDVN
                     IIDQKFNGKFVTQGTSGYIFSPTYATTMKDSIAIFYTYSDKTTGCSNTTTEYFKVLEA
                     TETIEDALPANRHYCYTDNLEARFTLKNIQEGSNNRNFELKGTDNIKSVDGNIVTVSI
                     NNTGFSKGIKTDTLIYTYKIDTVKFSVFKIFSIDNVSASLNFGVKDSFCTNGNDFVML
                     SFADNSYSPVGGTFTWYSSDPALLNSAGTAATFDPSKAIISSSKSYNANFYYDTLRVY
                     YTSPGGCHSNVLQKIYKISSPTTLTFGSGFIHNYNKDGAEIKLNAMPNGGTYYSGTLN
                     IRYDKTEDAYYLNPQSSVVDNNQKITYSFVNNAGCNSSVTELINIIKTEGFLSKNGTS
                     NDLPVIFCYTEAPFNIYGIAGNNKGPASKDTLYGEGVTYLQDNVALFSPSAVIDSDTT
                     DATTKTIRITYEYTGNDADATRFKVFKDVTVDKVGKLTVELDSNYCYSSKLQTIKASC
                     ETRGSGNIYLNYKAKNYGFANNGFSANFYSDTLINKKVVEYGAPAIVTFVMESNKGCK
                     DSVKKVFRVRETPQTRLRVPDLCFNEGKPIYIYDSTTNMLKDKIVEWVWKFGDNSKDS
                     VSNIFSRIMAHNYSGTGQKTIRLTQTTQYNCVGYDETTINLGLKPSSIFNWVDECSAK
                     DGSSKAIHFTDKSTAPDGDPIKKRYWSVNSENGKILYNDSTTNNYKDIAFTQPGNYQV
                     QLRVVTQKNCIDTATKIFHLRPWVIVADDMPYSENFENGNNGWYPSLSGSIDNTIWKL
                     GESETFGTPEGERKVWYTENTGLSNINASVTGPCMDFSWIKKPMIKLNIKKLMLQGYD
                     GAVLQYTTDITDTVWNNLGTVSEGINWFNSNSIEGKPGNTFTGWTGKNSEDEYVSAML
                     NLDNLKWQPYVRLRMAYGKKNTGAQGIAFDNVWIGNRSKVLMVEGFTSASLTDDLGNN
                     LNNYNGFDMLEMRYHTEGDDFYEQYSSGPNARSLYYKLSDFPAYIADGRIVYSPNSDT
                     LSKKILQASKFDISLKTEKSNAGITLKVTITPLETINAEVINLHVAVVEDSATITGSN
                     VVYRNVVRKLLPDPSGTRITHWQVGVAQEYDFSWLYYAGINPDKVKIIAFIQDSKTKE
                     IYQAATDDAVFNKTDVKEMNILPGTISLYPNPANNYVYLLLNQSSRMLCTVQVFNNMG
                     MLVDKTNFSPQSQMIQLNTQNYKNGVYFIKVFGSDKVFKTGKLIVTHK"
     gene            16293..17375
                     /locus_tag="PK252_09155"
     CDS             16293..17375
                     /locus_tag="PK252_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT14913.1"
                     /translation="MLFKNKINLCIVGLVLFIEVITYFVCFKKGPLAIFPLNNKFRVV
                     FYTDKNDGGESVITSTQIADSVLKVSFVLKKGFIRPFIGLSILPAGMNYIDLSGYNQV
                     KIEALGRNLVNMNFHIVSHYQGVKSNRLYCSSSFELDKAKNDYSLLLKNLKIPDWWYD
                     TNNYSPSDTLKPDWKKVSEINFATGLAPDVDIERELSIYSVSFVRDNTWLLVYMFLIL
                     AGLILMLYIIEYFRSVVSENKHEVTIKYKPVEVANKLGFQNNYLDYINEHFQNPELSL
                     EEISSKTGISQRKISEGIAAQFHCNVKTYINKIRISEAQRLLKETDLSISEIAFKTGF
                     SSPSNFNRVFKAISGKNPTEFSRKKG"
     gene            17714..18859
                     /locus_tag="PK252_09160"
     CDS             17714..18859
                     /locus_tag="PK252_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT14914.1"
                     /translation="MKFKLFQKIGVAVLAVILVACNGSKSNEAMNPSGFLIKRGVNLS
                     HWLSQDFGWAPKYSYINEHDIKFIDSIGYDHVRIPVDEIELWDENGKLIEKAVKCLTN
                     CLDWCEKYDLRAIVDLNTVRAHHFNAANEGGKITLWADSAAQANFVNLWVQLSDILNK
                     YPVNMVAYEILNEAVAPEHDDWNKLMNRTVAEIRKKEPERVLVIGPNMWQIAPNLKFL
                     KLPEGDKNIILSFHTYSPMAFTHYKASWTPMKYYNGPVHYPGQIITNEDYQKYIDTTN
                     AALVAQTADARDVYNKKRLLEVFQSGVEYAKSKNLQLYCGEFGCLPTVERKDRLQYYS
                     DMVSVLEENNIAWCDWEYKGDFGIYFFDTEKAISLRPDFELIHILLK"
     gene            18935..20455
                     /locus_tag="PK252_09165"
     CDS             18935..20455
                     /locus_tag="PK252_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT14915.1"
                     /translation="MKKKFNLLVFFLCLTLIANAQLTPQEALSQMMRGINIGNSLDAT
                     PNETSWGNPLIQEHYFDDIVSAGFTSVRIPVTWRFHVSKNAPYTINEKWLARVDTVVS
                     WGLKRGLFIIINLHHEAGLKATDTMTNLSAKADTLAKYDSIWSQVATHFKDKPDRLLF
                     EILNEPQTMKAASVDSFNVRALAIIRKTNPTRIVVFSGTSYTGSDLLLAAKIPDKNDK
                     YLMGYYHSYDPWSFAGEAKGTFGTASDISQMKNRFAQVSAWSVKNNIPVLLDECGTIK
                     KCDYNSRMVYYATFVEQALNYNIGFNVWDDNGDFQVYIRNNRKWNDLKDVIIHTYKES
                     PTQLKAVAAETSIALSWANRTSENDSIFLDRRTASTDFAPIAKLAPSAAQYVDTGLTP
                     KTTYYYRLRTNLKDSVDLYSYPIVASTLKPTSIEKAENTSFEVYPNPAASIVRVVNNE
                     NSSAVLDIYNVNGKKINTITLFKKETTISLKGLAKGNYIFVMATPKGSQSKQVIVQ"
     gene            complement(20577..22652)
                     /locus_tag="PK252_09170"
     CDS             complement(20577..22652)
                     /locus_tag="PK252_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT14916.1"
                     /translation="MNYRLIYSWMMVFCIFSKISFGADLPVVVEAESGVAGSSFAIKT
                     ESGVTYATIKYTVGGTTPDSATRVITYTITFPDSGTYDLYGRYWIGSAGANDDSYYYG
                     NGFGEKNLTNADDWVRANNIHNLGYTDNSQVVDGAGGAAYNVWKWINFSQYNGDEPPV
                     SFRVELGELTKTFQIAGREDGFLIDKFVFGRKGLYFTVDKLNKGEEGSKYPPGVEPLL
                     PPIAEGKSKFLGCAYGSDSKYQFEGYWNQVTPGNASKWGWVEGTRDVMNWKDLDEAYN
                     FARKNKFPFKLHTLIWGSQQPTWIETLDSAEQRAEIEEWFAALAERYDSVDYIDVVNE
                     PIHAKPDKTGAGNYIKALGGAGTTGWDWVITSFKLARQYFPKAKLLINEYSVTNEQKN
                     ANTYAQIINLLKKDNLIDGIGIQAHAFSTNVDVNTLKANLAILEATEVPIYATELDID
                     GPSDAKQLQDYQRIFPLFWDNEAVKGVTMWGFRPGMWRTDQKAYLIDADGIKRPALLW
                     LRAYVNGTFAPAKTINVSSANNVTSIDVLGDTLQMYAEVLPDTATIKNVVWSVSDKTI
                     ASISATGVLTPLKNGTVTVTASCMEYQSTVKGSMDITISNQPNAIEGATLAAFSVYPN
                     PVTDVLTLKNANNISRIVITNIAGQNILSLNKLATSNVNVSALQNGLYIIKAYSNDGS
                     YNMVKFIKK"
     gene            complement(22792..23604)
                     /locus_tag="PK252_09175"
     CDS             complement(22792..23604)
                     /locus_tag="PK252_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT14917.1"
                     /translation="MQNNNSYKTKAFVYTLLIYAAIIAILLYIKFRTPIPPYPEGGGG
                     PGMGLEVNLGSDIGTDEIAGLNTPQFVEEKETEKPTEPVLKQDYDEDESIKDIEKEQK
                     NKKEIKKKEKAIKQEPQKTEQKTPQLNEKALFKKKSNEQGDIPGSGSIYGKKGGDPNA
                     LPGNGDGIGNGTGNGTGPGKDGISFSLEGRNPLYLQIPSDKSQSEGKVVVEITVDKDG
                     RVINAIPGVKGSTTLDEQLLQIAKTAALQSKFNTKAGSTIQKGTITYRFVLQ"
     gene            complement(23604..24005)
                     /locus_tag="PK252_09180"
     CDS             complement(23604..24005)
                     /locus_tag="PK252_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT14918.1"
                     /translation="MNLRSKNKFRPEVSTSSLSDIMFFLMLFFLITSTLISPSVIKLT
                     LPSSKYHQTVRKTEISISITKELQYYINNDAVAPSNIEAELIKRITDKENVTVVIRCD
                     SSVPVQELVNILQTGNKLKIKMILATKAPNA"
     gene            complement(24017..24694)
                     /locus_tag="PK252_09185"
     CDS             complement(24017..24694)
                     /locus_tag="PK252_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT14919.1"
                     /translation="MNFYLLQIQVAAEAAKTQAEKSMTLWELTLKGGWIMIPLAILAF
                     VAIFIFIERFLAIRKAASFESNFIDRIKDYIHEGKIDAALNLCSSTDRPVARMIEKGI
                     KRIGRPIKEIEDSIEIVGKFEVYNLEKNLVILGVIAGIAPMFGFIGTIAGVIKIFYNI
                     SLADNISIGLIAGGLYEKMVTSAAGLLIGIIAYIFYHWLNVMIEKVVHKLELNAVNFI
                     DLLQEPA"
     gene            complement(24765..29219)
                     /locus_tag="PK252_09190"
     CDS             complement(24765..29219)
                     /locus_tag="PK252_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT14920.1"
                     /translation="MDKGIKKILKITGIFILSVLAIPLLVFLLLQTNRVQNYATQKIA
                     SILGSKAGTEISVGYVSIDFLNRVVLKDVLIKDWRKDTLIYSKKTVVGISDISYKKKY
                     IALSKVEFEKAYVHLYSDSLKNMNITYIIDNLTDTADTASSRWIIMFKQVGLKNSAFR
                     LDDYYKTKQDTGVNFSDMFLDSLNIRIKNMMISNGKLYCNINKLCFQEQSGFRLKSLT
                     GKMTIKSSCLHFDNLKIETDRSNIEAYMLYFDFAKFGDFKYIENKVDIDAKIKKSSCS
                     MADISMFATSLYGMNSIFEVSGKIEGKISNFQCKKFYFKIGKNTFFKGNTNIKGLPDI
                     EKTYMNIDVDNMNVDIDDIVSIKLPDNQKIETPDIVKQLKIVGFIGNFDGYINNFKTS
                     GDFKTPYGHLKADIQLNPEKKRKLTFVGSVSSDNFEPGLLFMKDSIVGKTQFSVNAKG
                     FLAESNKIFAQIDWNIKSVILKNIEFRDIIGNGTLDNKTFDGTVKVDDERLAFEVKGN
                     FNFSDTNPQFKFEANLEHANLLALNIMPQDSITNISFNVTANFKGNSINKFDGEIRLK
                     EVNFEKKDKKLNINKIEVYTTDNQFSNDLQFRSDFLDATIFGRYDIADIPGAIIRTIR
                     AFLPGSFYGYQSDEPLDDNEFMFNLKFKKTDSISKFFMPSVRISENSKISGLFFPADT
                     SMRIMVQAQYLKIGNIVFDTIFVKAASRPDSLVLQVQTANLWFGPKIKMNKFYFDNSS
                     KNDSSHILFFWKDSLKHKNKIKAEAWFTYTGPTQKPIWHFSSLPSNFYLLNENWQIGS
                     LSLLFDSTGISFDKFAMYNGDKKIEITGKVSSSASDTLNAKFSDIDLTMLNFFMSGSN
                     IFLNGKAKGQIAFTHPNNKAHINANININDFSLNHQIIGNTHAKLWWDPFAEKFYFTA
                     LASKENVPTLNTSGFITPATSEFNIETQLHSLSTKIFQPFVENLFHFHNGNINGQLYV
                     KGKTDNPQISGICNVDSGDFTFIPFNTRYTFNSTIQFQNNNIIIAKTKVYDQKQQSFD
                     FSGRVLSITRQPRFDLKLELKNNMVFNTSIPQAMPIYGTCYADGNVSITGTTDNLKID
                     ITQAKTKEKTIVAIPLGLTDLSQSSFLRFKTIQKEEEHNVQKDLFEAKDEKKIRQKPL
                     QLNIDLDATSDAELQLVFDPKVGDILYCRGNGNLKLEIDEAGNFKIRGDYNITEGEYL
                     FTLRNLISKKFILQDGASITFGGNPLDATLNMQAIYKTKASIYPLTLDESSGSNDKKR
                     IPVDCRLFVKGKLQNPDIRFDFDLPSASVADKDKVKSVVNTDQEMSQQFLSLLVMSSF
                     MTPSNSGRSQGNSMGVDMASSSIEMLSGQISDMLSNLSDKFDVGVAYRPGDKTYTTNE
                     VEVALSTQLFDDRVTINSSVDISNQENNNQKASNIVGDVNVEFKLTDKIKLKAFNRAN
                     DDPLQQDDIYTRGIGIVYREDFSNFKELKNKLFFKNKKAEKTDTLAKP"
     gene            29298..30314
                     /gene="tsaD"
                     /locus_tag="PK252_09195"
     CDS             29298..30314
                     /gene="tsaD"
                     /locus_tag="PK252_09195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT14921.1"
                     /translation="MSVTILAIESSCDDTSAAIIRDGVLLSNLIASQEIHNLYGGVVP
                     ELASRVHQQNIVPVVDLAFKQAQVGIDEINAVAFTRGPGLMGSLLVGTSFAKGFALSR
                     NIPLIDVNHLLGHVLVHFIKEADKPHVPPSFPFLCLLVSGGHTQIIVVKNHLEYEIIG
                     QTIDDAAGEAFDKCAKTINLPYPGGPFIDKLAQQGNPNAFTFAKPNIEDLDFSFSGLK
                     TSFLYFLRDRIHEDPDFIEKNKADLCASLQYTIVKILIDKLVKAAKLTGIKDVAVAGG
                     VAANSGLRNALTDVAVVKKWNLFLPSLKFTTDNAAMIGIVGYYKYLAGEFSDQSISPV
                     ARIN"
BASE COUNT         9463 a         5256 c         6140 g         9622 t
ORIGIN      
        1 gatagctttg attattttgg gattaataaa ttatcagcat accaagacta ttttgattga
       61 atcaaataaa tccaaaaata aaattattgc tgataaaatt aagaatattc ttgagtttca
      121 ggatatgaca tttagtattc ttgatgaaaa tatggctcaa cgaatgaacg gatatagtaa
      181 tgctattgta aacaggtatg ccgggaattc aaaaattctg gaaagtgctg atttgaatgc
      241 attacgttca gaaataggta tgaatcctct tatggaagac atttatgtaa ttaataaaga
      301 aggggtcatt gtaaatacaa cctttaagaa agataaaggg cttgatttat ttagttttgg
      361 tgaagcccat aaaaagtttc ttctaagtgt ttttgatggt ggtagatttg tgaatgaacg
      421 ttttgcaata gaagcaacta ccaaacgttt gaaaaaatat acctatcagc ctacaaggga
      481 taaaaaatac attattgaaa taggctcata ctcgaaagat gcagatagta ttatcaacct
      541 ggtgataaat accttaggca agtcagtagg gctctttatt atgaccgata agcctttttc
      601 tatcactgat gctgaggtgt ccgagaagca taagcatttg ttagataaag tgtttgttga
      661 gaaaggccaa tttactgttt ttgaagaaga aaatgataaa aagattgaat atacatttat
      721 caacctaaac agcaagccta gtggcttgta caaggggtcg gccattcaga tagttacaga
      781 ccgcaccgaa gaaatcaata tgcttcgccg tgagctcatt aagctcattt gtatttttgg
      841 ttttgctata atccttgttg ttattctcat atatagaaag accaagatta ttacttaccc
      901 aatcaagaag ttggtgctta atatcaaccg tattacccat ggacatttag atgagcgagc
      961 agaggttgtc ggaaataatg aaatagctac actttcaaaa caattcaata agatgattga
     1021 agagctggag agttattaca atgaattgga acagaaagtg aaggatcgta ctgccgagat
     1081 tatgcagcag aaagaagaga ttgaagcaca gcgtgatgcc cttgagcaac agcggaattt
     1141 gcttgcccag accaataaaa gccttgaaca tgcttatgta gagataagcg agcagaaaaa
     1201 acatattacc gatagtatag tttatgctaa gcgtatccag aatgccattt tgccccctga
     1261 tgaagtgatc cataaatatt tgcctgattc gtttatactt tacaggccta aggatattgt
     1321 tagtggtgac ttttattggc tttcagagaa agaaggcttg gtaatgatcg ctgctgttga
     1381 ctgtactgga catggtgtac ctggagcatt catgtctatt gttggtcata acaacctcaa
     1441 ttttgctgtc aatgtcagag gtgcccgtaa ggccggagat atactcaatg agcttaacca
     1501 ggctgttact gatactctca ggcaacagca tgggaatgct tctatccgtg acggaatgga
     1561 tatagccctt tgttgttttg actttgagag tcgtcgcctg aattttgccg gggcaaacaa
     1621 tccgctgatt atagtcagga acggtgaagc tataaaatac gatgcaacta aagcgcctat
     1681 tggtgcttat attggagaca agcttacaaa ctttaccaac aatgaaattg agctttcggc
     1741 tggagatatg tgttatattt tctctgacgg ttatgctgat cagtttggag ggcctgaaaa
     1801 taagaagttt ttgaaaagaa aactgattga gcttttggct gaaattagca cagagcctgt
     1861 aaataaacaa aaggaaatgc tcgaacaggc gcatgagctg tggaaaggcg agcacgagca
     1921 ggtggacgat attttgataa ttggaattag atgtaaataa aaaggaacaa aatgactaat
     1981 cctgaaagca atatattaat tattacttca gctaaagaag gtgttgcgta tgattgggct
     2041 ttaatgttat ctgaaagttt aggatcagcc attgcccagt tggcaggttt ttctccagcc
     2101 attaatgttg actctttgga taatgtcgat agtgagaatt ttttgaaaag caaatcggct
     2161 gtttatattg tccttgacaa tatgtatatt tcgaatcagc tgcaaataga gaaggttaga
     2221 cgttatacca aaattgataa cctgaactta ttttttatcc ttcctgcccc ccagaatgag
     2281 aaggaattga aaaacataac agacaaaaag attacctatt attgttttga tgaagacccc
     2341 gaaactggta tcgaacgccg atatagtgct tttgaacccc ctgacatatc taagcttttc
     2401 tggtcaaaag tattggatat agcttatgat tttctgcaaa agaaagggaa aagtgaggtt
     2461 cagattaaaa aggccgtata tctggctatt acaggcccag aactttttga ataccgtgat
     2521 gcaattagga gtgagcttat acatcgtgga tatactgttt ttcctgaagt gaatttgact
     2581 aatgacacag aaaaaattgc tgaagaagta tctgcttatc tttccaagag ttatatttca
     2641 atacacctca taggcagtaa atatggccac atcatccccg gaattgaatc atcattgcca
     2701 gagcttcagt gccggttgtc tgcaaaacat ttaaggttgc aggctgagag actgcagcac
     2761 caaacctttc aaagattagt gtggattcaa cccaagataa aacctattga agagaaacaa
     2821 aaacgttttc ttaacgcttt gagagtcgaa gaaaaaaatt tcaataccga gatccttcag
     2881 atatcgcttg aagatttaaa agttgtgctg cgtgaaaagc ttgattatat tgatggaaaa
     2941 ataaaaagta cagagcaaaa ccttaacagc gtttatatta tttatgaacc caaagattat
     3001 gtaaaggtgt tggagatagt tcaatatttt gaaaaacaaa atgttaaaac tttacttatt
     3061 gactttgtaa aaatggctga tactattgtt gcttcgcatt ataattatct tacagcatgt
     3121 tcttcggtta ttatttatga tagtggtaac aaccctcaat ggttgagcag caaaatgaaa
     3181 gaccttgtaa aagcgccggg atttgggagg aagaacccgt ttaaggtgaa agcaattttg
     3241 actaaaaata taaatggatt ttctggtacc gataatctgt tgcttgacgc aaataaaata
     3301 gatcctgcat tatcaatttt tgttcaaaaa ttaaaccaaa actgatggat gaatatgtaa
     3361 aaacggccaa ccttgaagca atatcagaaa taaaggctgt aatcaatcct tttcccggcc
     3421 taaggccttt tggtattgaa gaaagccact tgttttttgg acgcgaagga caaagcgaag
     3481 aagtgctatt gaagctttcc aaaaaccgtt ttgtggcagt tattggtgca tcggggacag
     3541 gaaagtcttc tcttatatat tgcgggttag ttcctatttt gtatggaggg ttcattgcta
     3601 atgctggttc gcaatggaaa atcattgcta ccaggcctgg aggtgggcct attgctaaca
     3661 tggccgaatc tgttgtgaaa tctgtttata aagatgaagc agacaaagaa aagcagatga
     3721 aagcttcctt ttttgatgct atgctccgca gaagttctct gggactgctt gaggtattgc
     3781 gcaacctgcc tatagccaaa gatgaaaata tccttatgct cgttgaccag tttgaagagc
     3841 ttttcaggta taggcttagc cgtaaagggc aggaggcagt caatgaatct gaagcttttg
     3901 ttaagctatt ggtcgaggct gtaaatcaaa cagaaatacc tgtatatatt gttattacta
     3961 tgcgttctga ttttattggc gaatgttcca attaccagga gcttacaacg ctgataaaca
     4021 gaagcaacta cctgattccg caaatgacac gtaatgactt tcgcgatgca attgtggggc
     4081 ctgttgctgt tggaaatgct aagatagaaa ccgctttggt tcaggaattg ctgaatgatg
     4141 ttggcgataa ccctgatcag ttacctattt tgcagcatgc catgatgcgt acatgggatt
     4201 attggctcaa taacagcgac cgggacaagg aagtcggctt gagcgaatac gaagccattg
     4261 gtaaaatgga aaaagcccta tctgaacatg ctaacgaagc ctttgatgag ctttcggaag
     4321 agggacaaag aatttgtgaa gtgctgttta aaactatcac agaaaaaggg gcagacaata
     4381 ggggagtacg tcggcctaca aggttagaag atattgcaga cattgctgct gctgatattg
     4441 atgaactgaa aactgtaatt gaaaaatttc gtgccccggg acgttctttt gttacgccat
     4501 ccagtgaagt gccgttgaca aaagactcta ttattgacct ttcgcacgaa agcttgatgc
     4561 gtatctggaa ccgtctgaaa gtatgggttg acgaagaggc ttctgctgtt cagatgtacc
     4621 ttaggctttc agaggcatct gcattgtttc aggaagggaa aacaggcctt tggagacctc
     4681 ctgatttgca aatagcttta aactgggaaa agaagcaaaa gccaacactg gcttgggcac
     4741 aaaggcataa ccctgcattt gagcgggcta tggtttttct tcataccagt gctaaggaat
     4801 atgaggccga tgagcttaat aaaatcaagc ttcagaaaag ggctttgcaa cgaagccgta
     4861 tgattgctgc tatacttggg gttgctgctg ttatctcact tggaattatg atttatgctc
     4921 agatgcaaag tactgaggcc tttaaacagc ggatggctgc tataaaaagt agcgtcgaag
     4981 ccaacaaaca aaagtctata gctttgaaaa atgcccagga ggctaatgaa caaaagaaaa
     5041 tagccgaaca gcaatccatt gaggccaata aacagaagga aatagctctt gagcaaactc
     5101 aaatagcttt gcagaatgca acagaagcca accggcaaaa gaatatcgct gatgctaaaa
     5161 gcaaagaagc taacgagcaa aaggtcctgg ccgaaaaaag tgctaaagaa gctttagaac
     5221 aaaaattagc tgcagaaatt gcccgtgaag aggcttacaa caggcgtatg ctttctatag
     5281 cccagtctat ggcagtaaag tcgcaacaga tagatgatga taaagatttg aaagcgttgt
     5341 tggcatttca ggcattcaca ttcaatgaga aataccaagg ttcagaaaag aaccctgatg
     5401 tttttgccgg gctttaccag gctatgatta ctttaaatgg gaaaagccag aatatttata
     5461 aaggccatag tgatgcaatc aattctattg ttttttggcc ttctgcaagt ttgttttaca
     5521 cagcaagtag tgatggtaag gttttaaaat gggatgtttc tgattcaact aaaaagcctt
     5581 ttgttgtcat tgacaataaa aaggtggtaa ataaatctat agacattagc agtgatggta
     5641 aatatcttgc agttggtaca ttgggcaatg gattggggat tttagacctg aaaacaaaag
     5701 atgcatcggt gcgttatgca gttgtacctt acaaaaatat tgaattagtt gcttttacca
     5761 atgatgctaa aacgattgtt gcagcatctg atagttctct gattgaattt gatgctacaa
     5821 caggagccgg aacggtcgta ggaaaagcag atggcaagat tctttcgtta gcagtggccc
     5881 cggatggtaa gactatagct accggcacaa aatcaggtaa agtatatctt tgggataaag
     5941 taagcagcta caaaaaagaa cttttatttg aagaagcgtc gaatcagata cattcgctgg
     6001 cttttaatca taatggctcg ttattggctg caggtaacct gataggttat ctcaagctat
     6061 ggaatttgaa ggaaaagaaa atgcttttca atgttaaagg tcattctgca cgtattacag
     6121 atattaagtt tagtccgaat gacaaaacta ttgctactgc aagctacgac caaaatgtgt
     6181 tgttgtggga tgcttctgat ttgaatattc agcctatcaa acttaaagaa catgagtcat
     6241 gggtactgtc attggctttt aatccgtttg gcgacaagct tgttactggc agtatcaaag
     6301 aagacaggct gattctttgg cctaccaata cttatgatat ggcttctgta gttcataaga
     6361 aaatgggacg aaattttact attgaagaat ggaatactta tgtaggaaag gacataagat
     6421 atgagcggtc taaataatta atatttaaaa caatctatag atgataaaag ttaagctttg
     6481 gatatttctg ttaagtatcg cttgcctgtt gccttttaaa gttcttggcc aggaagattg
     6541 tgcttttaca ttgtcgaaag cacaaaagtt gtatgatgca ggaaccattg aacagattcc
     6601 tgaaatgttg aagatgtgta tggaaagtgg ttttaccaaa gaagaaaaac agcaggcaat
     6661 gaagttgatt atcatggcat atttgtttga taacaatcag aaagaagctg ataaagagat
     6721 gcttaagttt cttaaagaat atccggagta tgaaattgtc gtttcagatc aggccgagtt
     6781 tgttcagctt tttaacacct atcgtacttt acctattgct tctattggat tggtggggtt
     6841 taccaatatg tcttatgtta ccccgataag ctattacgga acagatggaa ataaaggctc
     6901 atacaaaaat acaggctttg ggtttcaggt ggggctttcg tatctgcaat acctgatgga
     6961 gtatattgat ttaaacgtag aagggttcta tgtttccagt acttatcaat ttgccagctc
     7021 agatactgcc aagcaaataa catttgaaga aactcaatca aaacttgagt tcccaatatc
     7081 agctgtgttt aaacccagat cttttggtat gtttgtgccg tattgccgtt tagggtttgg
     7141 aacaagcctg cttttatcat ctaatggttc tgaaactact attaatcctg catctatggt
     7201 taaacctaat tcaaatccca gttttgattt aatgaacagg aggaaaaaat ttcagatata
     7261 cggtattatt ggggctggtg tgaggataaa cctttctcat agctttttat tattggatgt
     7321 caggtataat caaggcttca cacaacagtt taagaaatcg gccaggacct caggtgatta
     7381 tataaactat gtgataaaca cgcaacataa tgaaaatgat ttcagaatta ataatttatg
     7441 gatgtcaata ggtttctatt ataaatttta taaaccccaa aaacgataac tgatatgaat
     7501 aaatatatat actatattat attggttcta ctggcttttg tgtcatgtaa agaagctgat
     7561 atggcttcag atcaggaaaa atcgcatatt aaattttatg gaggaagcta tcaggctgaa
     7621 gggaacgata ttaaacaact ttcggacggc tgctttgttg cttttggaac aattagcttt
     7681 tctgaaatct ccaaaagtat gtacctcgtt aaaactgacc gacttggaaa cgagatttgg
     7741 agtaaagtaa taacagatac aattagtaaa actggcgtta gctttgaggt aatgcctgat
     7801 ggtgggtttg ttatgcttgg gaatacaatt acttctcctt caaggatttc tgttgcaagg
     7861 acagatgcaa atggagtaac tttatggaca aaatacatca gcaggggaaa aggaattaaa
     7921 gggtatagca tcaaaagaac atcagacaat ggattggtca ttgccggaag ttataccaat
     7981 gctaatgatg atttagatgc catgctcttt tttgtggatt ttgcagggaa caacgaaaaa
     8041 ttgattacag ctgcctggga tgaaaatgat gaggctaaag atgttataga acttggaaat
     8101 ggtattttta ttatggctgg ttacaggaca aatattagca caaaaggctc aaatgcttgc
     8161 ttctggattg ttaacagtaa agatgaagat ttcccttttt tacatggctt agttattgag
     8221 aaaaataaag tgagttctat tgaaagggtt aagtttattg gaaattctaa ttatttgatg
     8281 tgtggacagg ttaacactct tgattcggca atgaatacat ttgtggctaa aataaaagtg
     8341 ttctcacctg acaacacaac tgattatacc attaagcctg tttggaaaag aagttatggt
     8401 agtaaaaata aagactttgc aacgagttgt gaaatgcttt cagataatga ttttgctata
     8461 ttaggaacta cttataacga cctgggtcaa accagcatga cgctttataa aatagacagt
     8521 ctgggaaacg aaacctcagc taaaaaagaa tatggaggga cctcaggtag ttctgttagc
     8581 caatccggta actgtatatt gaaaactcag gatggaggcc ttgtcactac cggggttaat
     8641 tcatactcaa aaaccacttt gctgagcatt attaaatttg atagcaatgg taatttttga
     8701 ttttattctc atataatcaa ccagcctaaa tatactcact ttaaaacgta aagtcatgaa
     8761 acgagcatgt ttagaattaa attcaaatac gtatgaacaa aatcatgcga gttctttccg
     8821 acctttaaag aataattttg ttcggatgaa aacaaatata aatactatga aaagaacgat
     8881 tcgttttatg ttttttttct tcttgttatt gatgacacct gtattgcttt ttgctgaggg
     8941 tccaacattg agtagtgtta gtatagcatc gaataacagc gttttaactc aagcaaaggt
     9001 aggggacaag ataatgctga cctttacggg cagcgagccg ctgacaagca agccgacagt
     9061 gacgatatta gggcatatca tagccaatga aaatgttgtc aatacaagtg gcaatacttg
     9121 gacagcggaa tatactacat taaatagcga tacggagggt acagtgcctt ttactattaa
     9181 ttatacagat attgctggca atgctggaat agcagtaacg accgttacag atggcagctc
     9241 tgttactttt gataggactt tgccaacatt gagcaatgta aatattgcat cgaataacag
     9301 cgttttaact caagcaaagg taggggacaa gataatgctg acctttacgg gcagcgagcc
     9361 gctgacaagc aagccgacag tgacgatatt agggcatatc atagccaatg aaaatgttgt
     9421 caatacaagt ggcaatactt ggacagcgga atatactaca ttaaatagcg atacggaggg
     9481 tacagtgcct tttactatta attatacaga tattgctggc aatgctggaa tagcagtaac
     9541 tacagatggc agctctgtta cctttgatag aacggctcca acagttatta tttcgagttc
     9601 agcagcaaat ccgactaatg tttctccaat tccattaaca attactttca gcgaagatgt
     9661 tacaggattt gttgttgaag atattactac atctggaggt tctttatcag ggttttcagg
     9721 aagtggaaaa gtatatacgg ttaattattt tccagtttat ggaacaaaca atataaatat
     9781 tgctgctaat aaggttatag atattgcatc taatggaaat atagcagctt cccagttttc
     9841 tattgaatat tataaaacac ctgaagttga atttgttagc ggagatacta tttgtgctaa
     9901 tgaaacagta gttaatttat cagcaacagc aaaagataat cgtggcacaa tatattggta
     9961 tacagaagtt acaggcggaa ctcatgttgc ttctggcaac aattattctc ctaatataac
    10021 aacaactact tcatattatg ttgaggtcaa tgatcatggt tttatttcat cgcccaggat
    10081 taaggttgat gcgatagtat atccattacc gcttactact gcaacaaata ccgggcctgt
    10141 atgtgaaggt tctgctatta gtctggttgg aggcccgata acccccgata ttgtcaatta
    10201 ttcatggact tcaactccgg ctggttatac taattcatat catagccctc tattatcaag
    10261 caatgcaaca gaaggcctct ctaaaacata tactttgaaa actacagata ttcatggatg
    10321 tgttaatact gcaagtactg ttgttacagt aaatgcaacc ccaacaatat ataacgttac
    10381 agggggtggg gcatattgtg ctggaggacc aggaatgtca atcgggttag atggtgctga
    10441 taagaataca acatatactt taaaaagagg aataactcca gttgccacat ataattcagg
    10501 aacaaataca tcagctttta cttttggtac atttacacaa gccggtgaat atacagtagt
    10561 tgcaacaagt agttgtactg ctaatatgaa tggttcggta aatataactg tgaacagtct
    10621 gccaacagtt tacgcgttta acaatagtcc aatttgtgca gaaaattcat tagtgttaaa
    10681 tgcactgcct tcgggcttga attcttatag ttggactggc cctgctattt cagggtcatt
    10741 atcaggacaa agcataactc tgactaatgc tggtgcagta tctcacagtg ggacatatac
    10801 ccttactgtt acagatgaca acggttgtgt taatacggca acaactgttg caaaagtaaa
    10861 tgcactgcct tcaccatcta tagtagggct tgatagtgcc tataaaacta ccgattcaaa
    10921 tattgaatta gtaggagatc cttctggagg cacttttagc gggttagggg ttatttcaag
    10981 tatcaataaa tttttcccta atctggttag taaaattgga gaaagttttg atattaaata
    11041 tacctataca gatgaaaaga attgcactaa ggtagcaaag caaagtgtag tggtgcttaa
    11101 aacaagtgct attttcgaaa gtattaatcc taattcaacg tattgctata ctgatacctc
    11161 agatgaaata tgggcaaaaa gcccagacat tgttggtaat ggttattaca aacattccaa
    11221 aaaacatgga ttacaggatt tagggaataa tcatgctatt ttaactccgt ctttaatggg
    11281 tgccggaaat gatactataa catttttctt taaaactgtt gctggtgatg ataaaatctc
    11341 gagggtagtt tttattgata gtgttggtac tataagtatc aacactccgg ctgatgagtt
    11401 ttgtgaaaat gacaaccaaa ctgttacatt aaaaactgtt aatgctggca caaatggaac
    11461 taaaaactgg atatggcctg gtactgttgt taatggagac tatacctttg tgccaagcag
    11521 tttgaccaag aatacctctt ataattttag tgtaacctat gtttcaacta atggttgctc
    11581 aagtaatacg gctgatactg atatatttat taacgaaaag cctaatgata ctatatataa
    11641 cgaaaaaaca ttcaatgttt acaacgaggc agaagagtta aaaggtggca aagtctttcc
    11701 tttatattca tttagtgccc ctaaaggaat aacttatgat ggaagtgttt ataaatttaa
    11761 cccggctgta gtgcctgcca acacaagtgt tgttgttaca tatagttata cagacaataa
    11821 atcgtgtaca aatacagcta caaaaacttt caatatttat tcacctgtag gcactattac
    11881 caaccttggt tcggcaaaag atactttttg ttatggcgaa gcaattgctc actataagtt
    11941 taccacaact tccccatatt ctgctttatt attttatata gacaataaag attacacaag
    12001 ccaacttagc ggtaacgagg ttacctttcc gataggttca gatattggcc aagggttaca
    12061 tactgccagg ttcttatata aaaatgggca ttcgtggttt gaggttagta aaacttttta
    12121 tgtagaaaaa gcagtagctg gtacatttgt aatcaattat gccaatggta aagattatgc
    12181 ctgtcaggca gataataatg ttatcatcac ggtaaacgga tatgccccgg taggaccggc
    12241 aagctcgttt agttggacaa atgcattgaa tactacaatt tccagggcag aaatttctac
    12301 acagaatgca ggttcaatac cgtttagtta tgtttatatc tctccatttg gttgtcatag
    12361 cgaccccata gcaggttcag ttacaatcaa agctttgcct caggataaac tgggagtagg
    12421 aacaatatat aaccttgctg acggggcaaa accgcttaaa ggttctggtg atgttaatat
    12481 tattgaccaa aagttcaatg gtaagtttgt tacccaagga acatcaggat atatttttag
    12541 ccctacttat gctactacaa tgaaggatag cattgcaatt ttttatactt attcagacaa
    12601 aactacaggc tgttccaata ccactactga atactttaaa gttcttgaag ctacagaaac
    12661 gattgaagat gcacttcctg caaaccgtca ctattgttat acagataatt tggaagctcg
    12721 ttttactttg aaaaatatcc aggaaggaag caataataga aattttgagc ttaaagggac
    12781 agataatata aagagcgttg atggaaatat tgtaactgtt tcgataaata acacgggttt
    12841 ttcaaaaggt ataaagactg atactctcat ttatacttat aaaattgata cggtgaaatt
    12901 ctctgttttc aaaattttca gtatagacaa tgtatcagct tcgttaaatt ttggtgtaaa
    12961 agattcattc tgtaccaatg gtaacgattt tgttatgttg tcttttgcag ataacagcta
    13021 ttctcctgta ggaggaactt ttacctggta tagctcagac ccggcattgc tcaatagtgc
    13081 tggtactgct gcaacatttg acccttccaa agcaattatt agtagcagca agagttacaa
    13141 tgctaatttc tattatgata ctttgcgcgt ttattacacc tcacctggag gatgccatag
    13201 caatgtgttg caaaagatat ataaaattag ttctcctact actttgactt ttggaagcgg
    13261 ctttatccat aattacaata aagatggcgc agaaataaaa ctgaatgcta tgcctaatgg
    13321 cggaacttat tatagcggga cattaaacat tcgttatgat aagacagagg atgcttatta
    13381 cctaaatcct cagtcttctg ttgtggataa taaccaaaaa ataacctact catttgtaaa
    13441 taacgctggt tgtaatagct ctgttactga gttgattaat attattaaaa ctgaagggtt
    13501 cctttcgaaa aatggtacat caaatgattt acctgttata ttctgctata cagaagcacc
    13561 ttttaatatt tatggtatag caggaaataa caaaggccct gcatcaaaag atacattata
    13621 tggtgaaggt gtaacttatt tacaagataa tgtagctctg ttctcacctt cagcagttat
    13681 tgattcagac acaacagatg cgacgacaaa gactatcagg ataacatacg aatatactgg
    13741 taatgatgct gatgccacac ggtttaaagt atttaaagat gttacggttg acaaagtagg
    13801 aaaacttact gttgagttag attctaatta ttgttatagc tcaaaattac agaccattaa
    13861 agcttcatgc gaaaccaggg gaagtggtaa tatttatttg aattataaag cgaaaaacta
    13921 tggatttgcc aataatggat ttagtgccaa tttttatagt gatacactta taaacaagaa
    13981 agttgttgaa tatggagctc cagctattgt tactttcgtt atggagtcaa ataaaggatg
    14041 taaagattca gtcaaaaagg tgttcagggt tcgcgaaact ccgcaaacaa gactcagagt
    14101 acctgatttg tgttttaatg aaggaaaacc aatttatatc tatgattcaa ctaccaatat
    14161 gcttaaagat aaaatagttg aatgggtatg gaaatttggt gacaacagta aggattcagt
    14221 gtccaatata tttagccgta ttatggccca caattattca ggtacaggtc agaaaacaat
    14281 aaggttaacc cagacaactc aatataattg tgttggatat gatgaaacaa caataaacct
    14341 gggattaaaa ccttcttcaa tctttaattg ggttgatgag tgctcagcta aggatggttc
    14401 ttccaaagca attcatttta ccgataaatc aacagctcct gatggagacc cgattaagaa
    14461 aaggtactgg agtgtaaatt ctgaaaatgg caaaatactt tataatgatt cgactacaaa
    14521 taattataaa gacatagcct ttacccagcc cggcaattac caggttcaat taagggtggt
    14581 aacgcaaaaa aattgcattg atacagcaac taaaatattt catttaaggc catgggttat
    14641 tgtggcagat gatatgccat attccgaaaa ttttgaaaat ggcaataatg gctggtatcc
    14701 aagtttaagt gggtctattg acaataccat ttggaagtta ggagaaagtg agacttttgg
    14761 cactcctgaa ggcgaacgca aagtgtggta caccgaaaat actggtttgt ctaatattaa
    14821 tgcatctgta acaggaccat gtatggactt ttcgtggatt aaaaagccaa tgattaagtt
    14881 aaatataaaa aaattaatgc ttcaaggcta tgatggagct gtattgcagt acacaacaga
    14941 tattactgat acagtttgga ataaccttgg gacggtttca gagggaataa attggtttaa
    15001 ttctaactca attgaaggaa agcctggcaa tacgtttaca gggtggacag gtaaaaattc
    15061 agaggatgaa tatgtgagtg cgatgttaaa cctcgataat cttaaatggc agccttatgt
    15121 tcgtctgcgg atggcttatg gtaagaaaaa taccggtgcc caaggtattg cgtttgataa
    15181 tgtttggatt gggaacaggt cgaaggtatt aatggttgaa gggtttacat cggcttcact
    15241 tacagatgac cttggcaaca atctaaacaa ctacaatggc tttgatatgc ttgagatgag
    15301 atatcatacc gaaggggacg atttttacga gcaatattct tcagggccca atgcccggtc
    15361 gttatattac aagctctcag attttccggc ttatatagct gatgggcgta ttgtttattc
    15421 cccaaatagt gacacgctgt caaaaaagat tttgcaagcc tctaaatttg atatttcgct
    15481 aaaaactgaa aaaagtaatg ccgggataac tttaaaagta actataactc ctttggaaac
    15541 aattaatgca gaagttataa atcttcatgt agctgttgtt gaagattcgg ctacaattac
    15601 tggctccaat gtagtttaca gaaatgtggt gcgtaagtta ctgcccgacc cgagtggcac
    15661 ccggattacc cattggcagg ttggtgtcgc ccaggaatat gactttagct ggctctatta
    15721 tgccggcata aaccccgata aagtaaaaat tattgcattt atccaggata gtaagaccaa
    15781 agaaatatac caggctgcta ctgatgatgc tgtatttaac aaaacagatg taaaagaaat
    15841 gaacatcttg ccgggaacta ttagtttgta tcctaatcct gccaataatt atgtttatct
    15901 gttgttaaac cagtcttcgc gaatgctatg tactgttcag gtattcaata atatggggat
    15961 gctggttgat aaaacaaatt tctctcctca atcgcaaatg atacagttga atacccaaaa
    16021 ctacaaaaac ggggtatatt ttattaaagt ttttggttct gacaaggtat tcaaaacagg
    16081 aaagctgata gtgacgcata aataaaacaa gcgaggcagg cattttgacc tgcctcgctt
    16141 gcttttaagc atatattatt aatggcatta attttttgtt gtattcagat tcttttacat
    16201 ttgatatttt gttttaaact tgtggaagat attaatttaa gggttattct gaaacataaa
    16261 tggggcatat atctcatttt aaaatatttc gggtgttgtt taaaaacaaa ataaaccttt
    16321 gtattgttgg gcttgttttg tttatagagg ttattacata ttttgtatgc tttaaaaaag
    16381 ggcctttagc tattttccct ttaaataata agtttcgggt tgttttttac acggataaaa
    16441 atgatggtgg tgagtctgta attaccagta ctcaaattgc agattctgtt ttaaaagtaa
    16501 gttttgtttt aaaaaagggg tttatcaggc cttttattgg cttgtctata ttgccggcag
    16561 gtatgaatta tattgacctg tcggggtata accaagtgaa aattgaagcg cttggcagaa
    16621 acctggttaa catgaacttt cacattgtat cacattacca gggggtaaaa agcaacaggt
    16681 tgtattgttc atcaagcttt gaattggata aagcaaaaaa tgattattcc cttttactga
    16741 aaaacctgaa gatcccggat tggtggtacg acacaaacaa ttattcgcct tctgatacac
    16801 ttaagccgga ctggaaaaag gttagcgaaa taaactttgc aacaggtttg gcccctgatg
    16861 ttgatataga acgggaactt tctatttatt cagtatcttt tgtccgggat aacacatggc
    16921 ttttggtgta tatgtttttg atattggccg ggcttatatt aatgttgtac attatcgaat
    16981 atttccgttc tgtagtcagc gaaaacaagc atgaagttac gataaaatac aagcctgttg
    17041 aagtggccaa taaacttggc tttcagaata attatctgga ttacataaac gagcattttc
    17101 aaaaccccga actcagcctt gaagaaatat cttcgaaaac aggaatctct caacggaaaa
    17161 tttcagaagg gattgcagcc caatttcatt gcaatgtaaa aacttacatc aacaaaatac
    17221 gcatcagcga agctcagcgg ttgttgaaag aaaccgacct gagtattagt gagatcgcgt
    17281 ttaaaacggg ttttagcagc ccaagcaatt tcaaccgcgt attcaaggct atttcaggaa
    17341 agaacccaac agagttttca cgaaaaaaag gataattatt acatcaaatc ctgcaagtcc
    17401 tgataaagac ttggcatgtt tttaaattcc acaaatgtta aatttctcaa tgataatctg
    17461 ccgcaacgta taaaatgctg ataaatttgc agttacaaag aaaactaaaa gtttcggaag
    17521 aactcaaata tagtagtaat attttataca aaacacattc aaacaacact ttgcaatttt
    17581 tgtaaagcat ttctcatttt ttgtaaagct gtttttctga ttttgtaaat tcaaaaatga
    17641 aatccgtaaa gtcatttgtt ttttgaatga tgaatattgc attttcaata acaaataact
    17701 aactattatg caaatgaaat tcaaattgtt tcagaaaatt ggagttgcag tattagctgt
    17761 catcttagtg gcctgcaatg gaagtaagag caacgaagct atgaaccctt cagggttttt
    17821 aatcaaacgt ggtgttaacc ttagtcactg gttgtcgcag gattttggat gggccccaaa
    17881 atactcttat atcaatgaac atgatattaa gtttattgat agtattgggt atgaccacgt
    17941 acgaattccg gttgatgaga ttgagctttg ggacgaaaat ggaaagctca tcgagaaagc
    18001 tgtcaaatgc cttaccaact gccttgattg gtgcgaaaag tacgatttga gggctattgt
    18061 tgacctgaat actgtcaggg cgcaccattt caatgctgca aacgaaggtg gaaaaattac
    18121 tttatgggcg gatagtgccg cacaggcaaa ctttgtcaac ctatgggtcc aactatctga
    18181 tattcttaat aaatatccgg taaatatggt tgcatacgaa atattgaacg aagctgttgc
    18241 tcccgaacat gacgactgga acaagctgat gaacaggaca gtagctgaaa ttcgtaaaaa
    18301 agaacctgaa cgcgtgctgg ttattggccc taatatgtgg cagatagccc caaacttgaa
    18361 atttttgaag cttcctgaag gtgataaaaa tattattctg agttttcaca cttattcgcc
    18421 aatggctttt actcattata aagcaagttg gacacctatg aaatattaca acgggcctgt
    18481 tcattatccc ggacaaatta tcaccaatga agattatcaa aaatatatag ataccactaa
    18541 tgctgcctta gtagcccaaa cagccgacgc aagagatgta tataacaaaa aacggttgtt
    18601 ggaagtgttc caatctggcg tagagtatgc caaatctaaa aatcttcagt tgtattgtgg
    18661 cgaatttggc tgtttaccta ctgtagaacg aaaagacagg ctacaatatt actcagatat
    18721 ggtttctgtt ttggaagaaa acaatatagc ctggtgcgat tgggaataca aaggtgattt
    18781 tggcatttac ttttttgata ctgaaaaggc catatcgctc aggccagatt ttgaactaat
    18841 tcatatttta cttaaataac atattaatta ggtattgaaa aaaaatatat tataaaatgc
    18901 atttaatggt taaaacgcat aaataaaaaa agaaatgaag aaaaaattta atttgttggt
    18961 gtttttttta tgcctaacgt tgattgcaaa tgcacagttg acacctcagg aagctttaag
    19021 ccaaatgatg cggggcataa atattggcaa ctcattggac gctaccccga atgaaacatc
    19081 atggggcaat ccgcttatcc aggagcatta ttttgatgat attgtcagtg cgggttttac
    19141 ttctgtaagg attccggtaa cctggaggtt tcatgtatct aaaaatgccc catataccat
    19201 taacgaaaag tggttggcaa gggtagatac tgttgtttca tgggggttaa aaagaggctt
    19261 gtttatcatt atcaacctgc accacgaagc ggggcttaaa gctacagata ctatgaccaa
    19321 cctgtcagca aaagcagata ctcttgccaa atacgatagt atctggagcc aggtggcaac
    19381 acattttaag gataagcctg accgtttgtt gttcgaaata ctcaatgagc ctcaaacaat
    19441 gaaagctgcc agtgtagatt cttttaatgt aagggctttg gctatcatcc gaaaaactaa
    19501 cccaacccgt attgttgtat tttcaggcac ttcatatacc ggctctgatt tacttttggc
    19561 agctaagata cctgataaga atgacaaata cctgatgggg tattaccatt cttatgaccc
    19621 gtggagcttt gctggtgagg caaaaggtac attcggcaca gcatctgata ttagtcaaat
    19681 gaaaaacagg tttgcccagg tttctgcatg gtcggtcaaa aacaatatcc cggtactcct
    19741 tgacgaatgc ggaacaataa aaaaatgcga ctacaattca cgaatggttt actatgccac
    19801 ttttgttgag caggctttaa actataatat tggctttaac gtatgggacg acaatggcga
    19861 ttttcaggtt tatatcagaa ataacagaaa gtggaacgac ctgaaagatg tcattattca
    19921 tacttacaag gagtctccaa cacagcttaa ggcggttgct gccgaaacaa gtattgcttt
    19981 gtcttgggca aaccgtactt ccgagaatga cagtatcttc cttgatcgca gaacggcaag
    20041 tactgatttt gctcccattg caaagcttgc tccaagtgca gcccagtatg ttgataccgg
    20101 cctgacacct aaaacgacat attattacag gcttcgcact aacctgaaag attctgttga
    20161 tttgtattca tacccgattg tggccagtac tttaaagcca acatcaattg aaaaagctga
    20221 aaatacaagt tttgaagtat atcccaatcc ggctgcttca attgtaagag tagttaataa
    20281 tgaaaatagt agtgcagtat tggatattta caacgtcaat ggcaaaaaaa tcaatactat
    20341 tactcttttt aaaaaagaaa caaccatatc attgaaaggc ttggccaaag gcaattatat
    20401 ttttgttatg gctacaccca aaggttcaca gtctaaacag gtgatagtgc aataaaatta
    20461 atgttagtat agatgaaaag ccttttggcc taacaagcca aaaggctttt ttattcttca
    20521 aaaaaaaggg ccggaataac cggcccttct caaaaaatat caaaaacgat gtcaatttat
    20581 ttcttgataa atttgaccat gttgtatgaa ccatcatttg aataagcttt aatgatgtac
    20641 agcccgtttt gcaatgccga aacgttgaca ttgcttgtag ccagtttatt caatgaaagt
    20701 atgttttgac cagcaatatt tgtaataaca atgcgcgaaa tattgtttgc gtttttcaat
    20761 gttaatacat cggtaacagg gttggggtaa acgctgaaag ctgccaatgt agccccttca
    20821 atagcattag gttggttaga aatggtgata tccatcgaac ctttcactgt tgattggtat
    20881 tccatgcagg atgcagtgac tgttaccgtg ccgttcttta gcggagtaag aaccccggtt
    20941 gcacttattg acgcaatggt tttgtcgctg acactccata ctacattttt aatcgttgca
    21001 gtatcaggaa ggacttcagc gtacatttgc aatgtatctc ccaaaacatc aatagaggta
    21061 acgttattag ctgaagaaac attgattgtt ttagcaggtg caaaagtgcc gtttacataa
    21121 gcccttaacc aaagcaaggc aggacgtttt atgccatctg catctatcaa atatgctttt
    21181 tggtcagtgc gccacatgcc tggtctaaat ccccacattg taacgccttt tacagcctca
    21241 ttatcccaaa atagcgggaa tatacgttga taatcttgca attgtttagc atcgcttggc
    21301 ccatcaatat ctaattcggt agcatatatt ggtacttcag tagcttcaag tatagccagg
    21361 ttggctttta aagtatttac atcaacattg gtagaaaaag cgtgggcctg tatgcctatc
    21421 ccgtcaatga gattgtcttt tttcagcagg ttgatgattt gagcataagt attggcattt
    21481 ttttgctcat tagttacact atattcgttt atcaggagtt tagctttggg aaaatattga
    21541 cgagccagtt taaacgatgt aatcacccag tcccagcctg ttgtacctgc tccgcccaac
    21601 gctttgatat aatttccggc tccggtttta tcaggtttgg catgtatagg ctcatttaca
    21661 acatcaatgt agtcaaccga gtcataacgt tctgctaatg cggcaaacca ttcttcaatt
    21721 tcggcgcgtt gttcggccga atcaagcgtt tctatccatg ttggctgttg gcttccccaa
    21781 atgagggtat ggagtttgaa tggaaatttg ttttttcggg caaaattgta ggcttcatca
    21841 aggtctttcc agttcataac atctcttgta ccttctaccc agccccattt acttgcattg
    21901 ccgggagtaa cctggttcca gtatccttca aattggtact ttgagtctga accataagca
    21961 caaccaagga atttcgactt tccttctgcg atgggaggta acagtggttc tacgcccggg
    22021 gggtattttg agccttcttc gcctttgttg agtttgtcaa cagtaaagta aagccctttt
    22081 cgtccaaata caaatttgtc aatcaaaaag ccatcttctc ttcctgctat ttgaaatgtt
    22141 ttggtgagtt ctcccaattc aaccctgaat gagacaggtg gctcatctcc attgtattga
    22201 gaaaaattga tccatttcca tacattatat gctgcacctc cggccccatc cacaacttga
    22261 gaattatctg tatatcccag gttatggatg ttatttgccc ttacccagtc gtcagcattg
    22321 gtaaggtttt tttcgccaaa cccattgccg taataataac tgtcgtcatt agccccggcg
    22381 cttcctatcc aatagcgacc gtaaaggtca taagtgccag agtctgggaa ggtaatggta
    22441 taagtgatga cgcgcgttgc actatcgggg gttgtgccac ctactgtata tttgatggta
    22501 gcataggtaa ccccagattc ggttttaata gcaaagcttg aacctgctac ccctgattca
    22561 gcttctacta caactggcaa atcggcacca aatgatattt tgctaaaaat gcaaaatacc
    22621 atcatccatg aataaattaa tcgatagttc ataaaatgaa aatttagtta gttatatgag
    22681 tataagtata ggtacaaaag ttttaaaaat aaacttaaaa caggtggaaa aaaaagtcaa
    22741 taatgatgaa taaattgtta aaaggacacc ttttttttag aaagaaaaca attattgtaa
    22801 aacaaacctg tatgttatgg tgcctttttg gatggttgag ccagcttttg tgttgaattt
    22861 tgattgcagt gcagctgttt tggctatctg aagcaattgt tcgtcaagag tggtagagcc
    22921 ttttactccg gggatagcat taattacccg cccatccttg tcaacggtga tttcgactac
    22981 aactttccct tcactctgcg atttgtctga gggtatttgt aaataaagcg ggttacggcc
    23041 ttcgaggcta aatgatatgc catctttacc gggtccggta ccgttgcctg taccgttgcc
    23101 aatgccgtct ccgttgcctg gcaaagcatt cgggtctccg cctttcttcc cgtagatgct
    23161 gccgctgccg gggatatcgc cctgttcgtt gcttttcttt ttgaacaaag ctttttcatt
    23221 aagctgaggt gtcttttgct cagttttttg aggctcttgc ttaattgctt tttctttttt
    23281 ctttatttct tttttgtttt tttgctcttt ttcaatgtct ttaatacttt cgtcttcatc
    23341 atagtcttgt ttaaggactg gctctgttgg tttttcagtt tctttttctt caacaaattg
    23401 aggggtatta agcccagcta tctcatctgt gcctatatcg cttccgaggt ttacctcaag
    23461 ccccatgcca gggccccctc cgccttcagg ataggggggg ataggggtac gaaatttgat
    23521 atataacaaa atagctataa ttgctgcata aatcagcaag gtatatacaa aagcttttgt
    23581 tttatatgag ttattgtttt gcatcaggca tttggggctt tggtggccag tatcattttt
    23641 attttcagtt tattccctgt ttgtaaaata ttgaccagct cttgtaccgg aacagagctg
    23701 tcacatcgga tgactactgt tacgttttct ttgtcagtaa tgcgtttgat taattcagct
    23761 tcaatgttag aaggtgctac agcatcattg ttaatataat actgcaattc tttggtgata
    23821 gaaattgata tttctgtttt tcggacagtt tgatgatatt ttgaagaggg aagtgtcagc
    23881 ttgattacac ttgggcttat cagcgtgctg gtaatcagga agaaaagcat cagaaaaaac
    23941 ataatatcgc tcagcgaaga ggtacttact tccggcctga atttattttt actccttagg
    24001 ttcatgtgtt tgaaatttag gcaggttcct gaagaaggtc tataaagttt acggcgttta
    24061 actcaagttt atgtactact ttttcaatca ttacattgag ccaatgatag aagatgtaag
    24121 caataatacc tatcaagagg ccggcagcac tggtgaccat tttttcgtac aatcctccgg
    24181 ctataagccc tatactgatg ttatcagcca gtgatatgtt gtaaaatatc ttaataactc
    24241 cggcaatggt acctataaag ccaaacatcg gggcaatacc tgctataacg cctaatatta
    24301 ccaggttttt ttcaaggttg taaacttcaa atttacccac tatttcaata gaatcttcta
    24361 tttctttgat tggacggcca atacgcttga tgcctttttc aatcatgcgg gctacagggc
    24421 ggtcagttga gctgcaaagg ttaagtgctg catctatttt cccttcatga atataatctt
    24481 ttatgcgatc aataaaattg ctttcaaagc tggcggcttt gcggattgcc aaaaaacgtt
    24541 ctataaatat aaaaatagcc acaaaagcaa gtattgcaag cgggatcatt atccatccgc
    24601 cctttaatgt cagttcccac agcgtcatgc ttttttctgc ttgtgttttt gcagcttcgg
    24661 ctgcaacttg tatttgtaat aaataaaagt tcataatatt tatacttaag tttatttttc
    24721 agtgtctcaa aaatacaaat tttacatcac tattcaagtg tgtgttatgg ttttgccaga
    24781 gtatctgttt tttcagcttt tttgttttta aaaaacaact tgttttttaa ttctttaaag
    24841 ttcgaaaagt cctctctgta aacaattcca atccccctgg tgtatatatc gtcttgttgc
    24901 aaaggatcat cattggccct gttgaaggct ttcagtttga ttttgtcggt caacttgaat
    24961 tcaacgttta catcgcccac aatattgctt gctttctgat tattattttc ttgattagaa
    25021 atatctacac tgctgtttat tgtaacgcgg tcgtcaaata gttgtgtcga aagggccact
    25081 tctacttcgt tggtggtata tgttttgtct cctggcctgt aggctacacc gacatcaaac
    25141 ttatcgctga gattagacaa catatcactt atttggccag aaagcatctc aattgatgat
    25201 gaggccatat ctactcccat gctattacct tgcgatctgc cagagttgct tggggtcata
    25261 aaactgctca ttaccagaag agataaaaat tgttggctca tttcctggtc tgtattgaca
    25321 acacttttta ctttgtcttt gtcggcaact gaggcgctgg gaaggtcaaa atcgaacctg
    25381 atgtcggggt tttgaagctt cccttttaca aaaagcctgc aatctaccgg aattcttttt
    25441 ttatcattac ttccactgct ttcgtcaagt gttaatgggt atattgaagc ttttgttttg
    25501 taaatggctt gcatattgag tgttgcatca agtgggttgc ctccaaatgt tatagatgca
    25561 ccatcttgaa ggataaattt tttgcttatc aggtttcgta aggtaaaaag gtattctcct
    25621 tctgtaatgt tatagtctcc tctgattttg aaattgccag cttcatctat ttcgagtttt
    25681 aagttgccat tacctctgca gtataatata tctcctactt ttgggtcaaa tactaattgc
    25741 aattcggcat cagaagtggc atccaggtca atgttaagct gtaagggttt ttgcctgatc
    25801 tttttttcgt ctttggcttc aaacaaatct ttttgcacat tatgttcttc ttctttttgt
    25861 atggttttaa accttaaaaa tgagctttga gataaatctg taagccctaa aggaatggca
    25921 acaatggttt tttcttttgt tttggcctgg gtaatatcaa ttttgaggtt gtctgtggtc
    25981 ccggtaatgc taacattgcc atctgcataa caagtgccat atattggcat tgcttgaggg
    26041 attgaagtat tgaacaccat gttgtttttg agctccagtt tgagatcaaa gcggggttga
    26101 cgggttatgg acagtaccct tcccgaaaag tcaaatgact gttgtttctg gtcatatact
    26161 tttgttttgg ctataataat attgttattc tgaaattgta ttgtgctatt gaaagtatat
    26221 cttgtgttga aagggataaa ggtaaagtcg ccactgtcaa cattgcatat ccccgatatt
    26281 tgaggattgt ctgttttgcc ttttacatat agttgtccat taatgtttcc attgtgaaag
    26341 tggaacaaat tttcaacaaa aggttggaat atcttggtgg acaatgagtg tagctgagtt
    26401 tctatgttaa attctgaggt tgcaggggtg ataaaacccg aagtatttag tgtagggacg
    26461 ttttcttttg aagctaaagc cgtgaaatag aatttttcgg caaaggggtc ccaccagagt
    26521 ttggcgtgtg tgttaccaat aatttgatgg tttaacgaaa aatcattgat attaatattg
    26581 gcatttatat gggctttatt attgggatgg gtaaatgcaa tctgaccttt ggccttgcca
    26641 ttaaggaaaa tattcgagcc cgacataaag aaattgagca tggtcaggtc tatatccgaa
    26701 aattttgcat ttaacgtatc tgaggccgat gaagaaactt ttcctgttat ctctattttt
    26761 ttgtctccat tatacatggc aaacttgtca aaacttatgc cagtagagtc aaataagagc
    26821 gataagctgc caatttgcca attttcgttt agcagataga agtttgaagg taatgaacta
    26881 aagtgccata taggcttttg ggtagggcct gtataggtaa accatgcctc ggctttaatt
    26941 ttatttttat gttttaatga atctttccaa aaaaacaaga tgtggctcga gtcatttttt
    27001 gaagaattgt caaaataaaa tttgttcatt tttatttttg ggccaaacca taaattggct
    27061 gtttgtacct gtaaaaccaa tgaatcggga cgtgatgctg ccttgacaaa aatagtatca
    27121 aaaacaatat tgccaatttt caggtactgg gcctgtacca ttattcgcat tgatgtatct
    27181 gccggaaaaa acaacccact gattttgctg ttttctgaaa ttcgcaccga tggcatgaaa
    27241 aactttgata tggagtctgt ttttttgaat ttcaggttaa acataaattc attgtcgtct
    27301 aaaggctcat ctgattggta tccataaaaa ctgccaggaa gaaatgcccg gatagtcctg
    27361 attatagccc cggggatatc tgcaatgtca tatcgtccaa agattgtggc atctaaaaag
    27421 tctgaacgga attgtaagtc gtttgaaaac tggttgtctg tggtgtaaac ctctattttg
    27481 tttatattga gtttcttatc ttttttttca aagtttactt ctttcaatcg tatctcgccg
    27541 tcaaatttgt taatggagtt gcctttaaaa ttggctgtta cgttaaaaga tatgtttgta
    27601 atactgtctt ggggcattat attcaaagcc aacaagttgg catgctctaa attggcctca
    27661 aacttaaatt gagggttggt gtcgctaaaa ttgaaattac ctttgacttc aaaggcaagt
    27721 ctttcgtcat caactttgac tgtcccatcg aatgttttat tatcgagagt tccattcccg
    27781 ataatgtcac gaaattcaat gtttttcagg attacgcttt tgatattcca gtcaatctgg
    27841 gcaaaaattt tgttgctttc ggccaagaaa ccttttgcat ttacagaaaa ttgagtcttt
    27901 ccaactatac tgtctttcat aaatagtaaa cctggctcga aattatcgga gcttacgctc
    27961 ccaacaaaag taagtttcct ttttttctca gggtttaact gtatatcggc ttttaaatgc
    28021 ccgtatggcg ttttaaaatc gccagatgtt ttgaaattat tgatataccc atcaaaatta
    28081 ccaataaagc caactatttt taattgtttt actatatcag gagtttcaat tttttgatta
    28141 tcagggagtt ttatactaac aatgtcgtca atgtcaacat tcatgttatc aacatcaata
    28201 ttcatgtaag ttttttcaat gtccggtaac cccttgatgt ttgtatttcc tttaaagaaa
    28261 gtgtttttac ctattttgaa gtaaaatttt ttacactgaa agtttgaaat tttgccttca
    28321 atttttcctg aaacttcgaa aatactgttc atcccatata gcgaagttgc aaacatagaa
    28381 atatcggcca tcgagcaaga tgattttttt attttagcat ctatatcgac tttgttttct
    28441 atgtacttaa aatcaccaaa cttggcaaaa tcaaaataaa gcatatatgc ttcaatgttg
    28501 gaacggtcgg tttcaatttt caggttatca aaatgcaaac aggagctttt tatggtcatt
    28561 ttgcctgtaa gcgatttcag cctgaaccca gattgttcct gaaagcacag cttgttgatg
    28621 ttgcaataga gtttcccatt gcttatcatc atgtttttta tcctgatatt caggctgtcc
    28681 aagaacatat ccgagaagtt tacgccagta tcttgcttgg ttttgtaata atcatcgagc
    28741 ctgaaggctg aatttttcaa gcctacctgt ttaaacataa taatccagcg tgaagatgcg
    28801 gtatctgcag tgtctgtaag attgtcaatg atatatgtta tgttcatatt tttgagcgaa
    28861 tctgagtaaa gatgaacata ggctttttca aattcaactt ttgacagggc gatgtatttt
    28921 tttttgtatg aaatgtccga aattccaaca acagtctttt ttgaatatat cagggtgtct
    28981 tttcgccagt cttttatgag tacatctttc aataccacac ggtttaggaa gtcaatagat
    29041 acataaccga cagatatttc cgttccggcc ttgctgccca gtatcgaagc aattttttgg
    29101 gtggcataat tttgtacacg gttggtttga agcaataaaa ataccagcaa aggtattgcc
    29161 aatacagata ggatgaagat gccggttatt ttgagtattt ttttgatacc tttgtccaat
    29221 tgcaacagtt tttccgttgg caatataacg taaaaaaagc taaaaatggt tgcaaaaaat
    29281 tcataaattg ttgggatatg agcgttacaa ttttggccat cgaatcgtcc tgcgatgata
    29341 cttcagcggc aattatccgc gatggggtat tattgtctaa tctgattgcc agccaggaaa
    29401 tacacaactt gtacgggggc gtggtgcctg agcttgcatc aagggtgcac caacaaaaca
    29461 ttgtacctgt tgttgatttg gctttcaaac aagctcaagt cggcattgac gagataaatg
    29521 ctgttgcttt tacccggggc ccaggcctga tggggtcttt actggtaggt acatcgtttg
    29581 ccaaaggttt tgccttatcc cgcaacattc cacttattga tgttaaccat ctgttggggc
    29641 atgtgcttgt gcattttatc aaagaagctg ataaacctca tgtccctcct tcttttcctt
    29701 ttctttgcct gttggtttca ggcggacata cacagattat tgtagttaaa aatcaccttg
    29761 aatacgagat tattggccaa accattgatg atgctgccgg tgaggccttt gataaatgtg
    29821 caaaaacaat caacctgcct tatcccggag ggccgtttat tgacaaactc gctcaacagg
    29881 gcaaccccaa tgcttttact tttgcaaaac ccaatattga agaccttgac tttagcttct
    29941 ctggcttaaa gacttctttt ctttattttt tgcgtgacag aattcatgaa gaccctgatt
    30001 ttattgaaaa aaacaaagcc gatttgtgtg cttcccttca atatactatt gtaaaaattt
    30061 tgatagacaa attagtgaag gccgcaaagc tgactgggat taaagatgtt gccgttgctg
    30121 gcggtgtggc tgccaattcg ggattgcgta atgccttaac agacgtggct gttgtaaaaa
    30181 aatggaacct ttttctgcct tcgttgaaat ttactacaga taatgccgca atgattggta
    30241 ttgtagggta ttacaaatac ctggccggtg aattctcaga ccagtctatc tcgcctgttg
    30301 cacgcattaa ctaagttaaa acaggcataa attcttcttt tctattaatt cgtaaaaagg
    30361 ctgttgatac gaaagactaa tggggatatt gtttttccca atgataatcc gtttcttttc
    30421 tatcgtatct cattactgtc ccattaaaat ctaaaagagt tctttgaaaa gtttgaaatt
    30481 t
//
LOCUS       DAOSPF010000042        29445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1588_length_29445_cov_7.744301, whole genome shotgun sequence.
ACCESSION   DAOSPF010000042 DAOSPF010000000
VERSION     DAOSPF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_29445_cov_7.744301"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /gene="rpoA"
                     /locus_tag="PK252_09200"
     CDS             complement(<1..605)
                     /gene="rpoA"
                     /locus_tag="PK252_09200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT14922.1"
                     /translation="MAILAFQKPDRVIMVSATETSGQFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITSIKIEGVDHEFSTIKGVIEDVTEIILNLKQVRFKRVVEDVDHEKIHV
                     NFAGGSVFKAGDLNKFLNGFQVLNTEQVICHLDPSVKLQMELNINKGRGYVASEENKP
                     VNSEIGLIAIDSIFTPIKNVKYAIENYRVEQKTDYEKLVFE"
     gene            complement(614..1222)
                     /gene="rpsD"
                     /locus_tag="PK252_09205"
     CDS             complement(614..1222)
                     /gene="rpsD"
                     /locus_tag="PK252_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT14923.1"
                     /translation="MARYTGPKTKIARIFGEPIFGPDKNLEKRNYPPGQHGLARKRKK
                     VSEYGLQLREKQKAKYTYGVLERQFSNLFVKAQASKGVTGEVLLQLLESRLDNVVYRL
                     SLANTRNGARQLVLHKHITVNGEIVNIPSYIVKPGDVISVREKSKSLEVINDALKVAR
                     YNRFSWLEWNKDTFSGKYMNVPQRSDIPENIKEQLIVELYSK"
     gene            complement(1261..1650)
                     /gene="rpsK"
                     /locus_tag="PK252_09210"
     CDS             complement(1261..1650)
                     /gene="rpsK"
                     /locus_tag="PK252_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT14924.1"
                     /translation="MAKKTGTSKKKVVKVESTGQAHIHASFNNIIISLTNNEGQVIAW
                     SSAGKMGFKGSKKNTPYAAQTAAETCGKTAYDAGLRRVKVFVKGPGAGRESAIRSIHA
                     CGIEVTEIVDVTPMPHNGCRPPSRRRV"
     gene            complement(1664..2044)
                     /gene="rpsM"
                     /locus_tag="PK252_09215"
     CDS             complement(1664..2044)
                     /gene="rpsM"
                     /locus_tag="PK252_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT14925.1"
                     /translation="MARIAGVDLPKNKRGVIGLTYIYGIGVSSAKDILSQAGIDEDTK
                     VKDWNDDQLSKIRSIINEQYKVEGELRSMVQLNIKRLMDIGCYRGLRHRVGLPVNGQR
                     TKNNARTRKGKKKTIANKKKATKG"
     gene            complement(2078..2194)
                     /gene="ykgO"
                     /locus_tag="PK252_09220"
     CDS             complement(2078..2194)
                     /gene="ykgO"
                     /locus_tag="PK252_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOT14926.1"
                     /translation="MKVRTSIKKRSEDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(2207..2425)
                     /gene="infA"
                     /locus_tag="PK252_09225"
     CDS             complement(2207..2425)
                     /gene="infA"
                     /locus_tag="PK252_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT14927.1"
                     /translation="MSKQASIEQDGTITEALSNAMFRVELENGHIITAHISGKMRMHY
                     IKLLPGDKVKVEMSPYDLSKGRISFRYK"
     gene            complement(2427..3212)
                     /gene="map"
                     /locus_tag="PK252_09230"
     CDS             complement(2427..3212)
                     /gene="map"
                     /locus_tag="PK252_09230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT14928.1"
                     /translation="MIAIRTQEEIEKLRASNELVAKTLGELAKYIKAGVSTNYLDKIA
                     EEYIRDHGGIPAFLGYGGFPKTLCTSLNSAVVHGIPDDKTILNEGDIISVDCGVVLNE
                     YYGDSAYTFAVGEITERVKHLLKVTKNSLFEGISEAREGKRIGDIGFKVQTYCENAGF
                     SVVREMVGHGIGRKLHEAPEVPNYGKKGTGPMMKTGMVICIEPMINMGTRKIHQERDG
                     WTIRTWDNLPSAHFELAVAIEKEKGDILGTFKYIEEVLGDKEF"
     gene            complement(3242..4564)
                     /gene="secY"
                     /locus_tag="PK252_09235"
     CDS             complement(3242..4564)
                     /gene="secY"
                     /locus_tag="PK252_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT14929.1"
                     /translation="MKQLIDTLKNIWKIEDLKARILYTLGIILIYRLGSFIVLPGIDP
                     VMLSDLKRQTAQGVMGLLDMFSGGAFSNASIFALGVMPYISASIVMQLLGMAVPYYQR
                     MQKEGESGRRKMNQHTRLLTVIILLLQAPAYLTNLHVQYQGAIYGGRMFDITGVILLA
                     AGTIFIMWLGERITENGIGNGISMLIMVGIIARLPESFLQEFASRLDQKGGGLVVLLL
                     EIIILFVVFAGTILLIQGTRKIPVQYAKRIIGNKQYGGVRQYIPLKVNAAGVMPIIFA
                     QALMFIPITVAGFANSEKASGFVAAFANYTGFWYNFTFGLLILVFTYFYTAITVNPNQ
                     MAEDMKRNGGFIPGVKPGKKTAEFIDSVMSKITLPGAIFLAIVAILPAFAMKAGMTSG
                     FAHFYGGTSLLIMVGVILDTLQQIESHLLMRHYDGLMKTGRIKGRSGI"
     gene            complement(4561..5013)
                     /gene="rplO"
                     /locus_tag="PK252_09240"
     CDS             complement(4561..5013)
                     /gene="rplO"
                     /locus_tag="PK252_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT14930.1"
                     /translation="MNLSNLKPAQGSVRTNKRLGRGQGSTDGGTAGRGHKGAKSRSGY
                     SRKRGFEGGQMPMQRRLPKFGFTNPNRIEFKAINLEVIQKLAEAGLTVISPELLLEKG
                     LINKNSLVKILGKGELKAKVEVTAHAFSKSAEEAIKAANGTIVKIEAQ"
     gene            complement(5044..5223)
                     /gene="rpmD"
                     /locus_tag="PK252_09245"
     CDS             complement(5044..5223)
                     /gene="rpmD"
                     /locus_tag="PK252_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT14931.1"
                     /translation="MSKLKVTLVKSKSRVCERQLRTIKALGLGRRTSSVVVESNDVFK
                     GMISKVHHLVKVEEV"
     gene            complement(5235..5750)
                     /gene="rpsE"
                     /locus_tag="PK252_09250"
     CDS             complement(5235..5750)
                     /gene="rpsE"
                     /locus_tag="PK252_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT14932.1"
                     /translation="MSESIRKIKTSDLELKDRLVSIQRVTKVTKGGRAFSFSAIVVVG
                     NENGIVGYGLGKANEVTTAISKGIEDAKKNLIKVPIYKGTIPHEQFAKFGGASVFIKP
                     ASSGTGVKAGGAMRAVLESVGIHDVLAKAKGSSNPHNLVKATFQALLELRDPMAVAAS
                     RGISVEKVFKG"
     gene            complement(5755..6114)
                     /gene="rplR"
                     /locus_tag="PK252_09255"
     CDS             complement(5755..6114)
                     /gene="rplR"
                     /locus_tag="PK252_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT14933.1"
                     /translation="MSLTKQERRARIKHRIRKVVFGTAEKPRMTVFRSNKQIYVQCID
                     DSQGITLLAASSLQKEIAEASKGKKIDQAQLVGKKIAELCKQKGIAEVVFDRNGYLYH
                     GRVKVLADAAREGGLKF"
     gene            complement(6130..6684)
                     /gene="rplF"
                     /locus_tag="PK252_09260"
     CDS             complement(6130..6684)
                     /gene="rplF"
                     /locus_tag="PK252_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT14934.1"
                     /translation="MSRIGKLPINLPKGVAINVSPSNEVTVKGPLGELKQQVDADIKV
                     EVVENQVVVTRPTDQKRHKAMHGLYRALLANMIKGVSEGFKVEQELVGVGYRAEVKGQ
                     HLEMSLGYSHEILMVLPKEIKAEARTEKRANPIITLQCADKQLLGVVAAKIRSLRKPE
                     PYKGKGIKFVNENIRRKAGKTAGK"
     gene            complement(6697..7092)
                     /gene="rpsH"
                     /locus_tag="PK252_09265"
     CDS             complement(6697..7092)
                     /gene="rpsH"
                     /locus_tag="PK252_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT14935.1"
                     /translation="MTDSIADFLTRIRNAIRAKHRIVEIPASNLKKEITKILFDKGYI
                     LNYKFEDNGPQGVIKIALKYNPADKSSAIKCIKRVSRPGLRNYKSCEDMPRVLNGLGI
                     AIVSTSQGVLTDKEARLRNIGGEVLCYVY"
     gene            complement(7117..7386)
                     /gene="rpsN"
                     /locus_tag="PK252_09270"
     CDS             complement(7117..7386)
                     /gene="rpsN"
                     /locus_tag="PK252_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOT14936.1"
                     /translation="MAKESMKAREKRRMKLVEKYAAKRAQLKAEGDFVGLQLLPPNSN
                     PIRVHNRCMLTGRPRGYMRQFGISRIQFREMASKGLIPGVKKASW"
     gene            complement(7390..7947)
                     /gene="rplE"
                     /locus_tag="PK252_09275"
     CDS             complement(7390..7947)
                     /gene="rplE"
                     /locus_tag="PK252_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT14937.1"
                     /translation="MKYIPNLKTKYTNDIVPALKKEFNYTSVMQVPRLNKIVINQGVG
                     AAVADKKLIEVALNELTQISGQKAVQTVAKKDISNFKLRSKMPIGAKVTLRGDRMYEF
                     LERLIAVALPRIRDFKGVNAKFDGRGNYTLGLTEQIIFPEIDIDKITKMMGLEISFVT
                     TATSDEEGLALLREFGIPFKSAKKK"
     gene            complement(7947..8270)
                     /gene="rplX"
                     /locus_tag="PK252_09280"
     CDS             complement(7947..8270)
                     /gene="rplX"
                     /locus_tag="PK252_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT14938.1"
                     /translation="MQKKLHIKKGDIVKVIAGDDKGRSGRILEVNREKETAIVESINM
                     VSKHTKPNAKNPQGGIVKQEAPVHISNLMLIDPKSSVPTRIGRRLNAEGKLVRYAKKS
                     GEEIK"
     gene            complement(8289..8654)
                     /gene="rplN"
                     /locus_tag="PK252_09285"
     CDS             complement(8289..8654)
                     /gene="rplN"
                     /locus_tag="PK252_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT14939.1"
                     /translation="MIQQESRLTVADNSGAKEVLCIRVLGGTRRRYASVGDKIVVSIK
                     SALPSGEVKKGSVSKAVIVRTKKEIRRADGSYIRFDDNAVVLLNNADEIRGTRIFGPV
                     ARELRDKYMKIVSLAPEVL"
     gene            complement(8657..8914)
                     /gene="rpsQ"
                     /locus_tag="PK252_09290"
     CDS             complement(8657..8914)
                     /gene="rpsQ"
                     /locus_tag="PK252_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT14940.1"
                     /translation="MEERNLRKERIGVVVSNKMDKTIVVSVKRKMKHPIYHKFVNKTS
                     KFYAQDDNNICSIGDTVRIMETRPLSKSKRWRLVEIIEKVK"
     gene            complement(8924..9127)
                     /gene="rpmC"
                     /locus_tag="PK252_09295"
     CDS             complement(8924..9127)
                     /gene="rpmC"
                     /locus_tag="PK252_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT14941.1"
                     /translation="MKTSEIRELSTKELQERIDAEKSMLTKLRINHAVTPLENPMKIR
                     HTRRDIARMATELSMRELSDIKK"
     gene            complement(9134..9559)
                     /gene="rplP"
                     /locus_tag="PK252_09300"
     CDS             complement(9134..9559)
                     /gene="rplP"
                     /locus_tag="PK252_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT14942.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNTIAFGSFAIKTEESCWING
                     RQLEAARQAVSRYMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVYPITPGRI
                     IIEVEGVSQEVAKEALRLGAQKLPVSTKFITRRDYSETI"
     gene            complement(9649..10293)
                     /gene="rpsC"
                     /locus_tag="PK252_09305"
                     /pseudo
     CDS             complement(9649..10293)
                     /gene="rpsC"
                     /locus_tag="PK252_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(10298..10711)
                     /gene="rplV"
                     /locus_tag="PK252_09310"
     CDS             complement(10298..10711)
                     /gene="rplV"
                     /locus_tag="PK252_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT14943.1"
                     /translation="MGTRKRKMAESLKEAKRNKAIAVLNNCPSSPRKMRLVVDLIRGV
                     EVNKALHILKYSPKEASKKVEKLLLSAIANWQAKNEGVRLEDANLLVKTAFVDGGRML
                     KRLRPAPQGRGYRVRKRSNHVTLVLESANVASQTK"
     gene            complement(10741..11010)
                     /gene="rpsS"
                     /locus_tag="PK252_09315"
     CDS             complement(10741..11010)
                     /gene="rpsS"
                     /locus_tag="PK252_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT14944.1"
                     /translation="MSRSLKKGPYINFKLEQKVLALNNENKKSVVKTWARASVISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHSGNRKE"
     gene            complement(11034..11855)
                     /gene="rplB"
                     /locus_tag="PK252_09320"
     CDS             complement(11034..11855)
                     /gene="rplB"
                     /locus_tag="PK252_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT14945.1"
                     /translation="MGLKKFKPVTPGQRGKILVDFTEITKSTPEKSLLTSVKSSGGRN
                     NTGKMTMRYIGGGNKKMYRIIDFARNKDGIVATVKGIEYDPNRTARIALLVYADGEKR
                     YILAPEGLKEGQKVISGAGVEPELGNALPLSDIPLGTIIHNIELRPGQGGAMARSAGS
                     YAQLTSRDGKFAIIKLPSGETRMVLVTCKATIGAVSNSDHSNQKLGKAGRKRWLGRRP
                     RNRGVVMNPVDHPMGGGEGRASGGHPRSRKGLPAKGYKTRWKNKDSNKYIIERRK"
     gene            complement(11869..12159)
                     /gene="rplW"
                     /locus_tag="PK252_09325"
     CDS             complement(11869..12159)
                     /gene="rplW"
                     /locus_tag="PK252_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT14946.1"
                     /translation="MDILIKPIVTEKMTKIGEKQNMYAFKVKKNANKLQIKKAVEDMY
                     GVSVESVNTLIVGGKNKSRYTKTGFIQGRSSSYKKAIITVANNDKIDFFSNI"
     gene            complement(12172..12795)
                     /gene="rplD"
                     /locus_tag="PK252_09330"
     CDS             complement(12172..12795)
                     /gene="rplD"
                     /locus_tag="PK252_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT14947.1"
                     /translation="MKISVVNISGADTGRSVELKDEIFAIEPNDHAIYLDVKQYLANQ
                     RQGTHKAKERAEVAYSTKKIKKQKGTGGARAGSIKSPVFVGGGRVFGPRPRTYGFKLN
                     KKVKDLARFSALSYKAKENSLVVLEDFSFDVPKTKDFIKMRSNLKIDNKKMLLVLSEP
                     NKNVYLSSRNLQDAKVVTVSELNTYDIVNAGILVLSEKTIGALQTNA"
     gene            complement(12824..13438)
                     /gene="rplC"
                     /locus_tag="PK252_09335"
     CDS             complement(12824..13438)
                     /gene="rplC"
                     /locus_tag="PK252_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT14948.1"
                     /translation="MPGLIGKKVGMTSFISEEGKQIPVTVVEAGPCVITQVKTVEKDG
                     YQAVQLAYDDKKEKHTTKALLNHFKKANTAPKRKVVEFEGFEDAKLGDVISTDLFQVN
                     DFVDIKGQSKGKGFQGVVKRHGFSGVGGQTHGQHNRLRAPGSMGASSFPSRVWRGKRM
                     AGHTGNVNVKALNLQIVKIIPENNLLLIKGSIPGAKGGYVIIEK"
     gene            complement(13458..13763)
                     /gene="rpsJ"
                     /locus_tag="PK252_09340"
     CDS             complement(13458..13763)
                     /gene="rpsJ"
                     /locus_tag="PK252_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT14949.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSADKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLLDIYSSTPKTIDALMKLELPSGVEVEIKV"
     gene            complement(13776..15884)
                     /gene="fusA"
                     /locus_tag="PK252_09345"
     CDS             complement(13776..15884)
                     /gene="fusA"
                     /locus_tag="PK252_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT14950.1"
                     /translation="MARDLSYTRNIGIMAHIDAGKTTTTERILYYTGVNYKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCFWKYNNQEYKINIIDTPGHVDFTVEVERSLRVLD
                     GAVALFCAVGGVEPQSETVWRQANKYNVPRIGFVNKMDRTGADFFEVVQQVKDRLGAN
                     AVPFQIPIGAEDKFIGVVDLFENKAHIYDPADPKGEKFNIVDVPADLKETVEEYRDKL
                     LEAICEMDDALLEKFFENKDALTVEELKGVARKATIAMKITPIMCGASFKNKGVQQLL
                     DAVAAYLPSPLDVPNIEGKNPETDEIVVRTPDEKEPLSALAFKIATDPFVGRLCYVRV
                     YSGTITAGSYVLNSRTGKKERISRLFQMHVNKQNPRDIIEAGDICAVVGFKEIRTGDT
                     LCDEKAPIALESMTFPEPVIGIAVEPKTQNDLDKMGMALAKLAEEDPTFRVNTDEDSG
                     QTVISGMGELHLEIIIDRLKREFKIECNQGAPQVAYKEAINGSVQHREVFKKQSGGRG
                     KFADIIVKVEPADPGVVGLQFINEVKGGNIPKEYIPSVEKGFKEAMRNGVLAGYTMDS
                     LKVTLLDGSYHPVDSDALSFEICAKMAFKEACSKANPILLEPIMKLEVVTPEEYMGDV
                     IGDLNKRRGNVEGMEMKAGGRVIKAKVPLAEQFGYVTVLRTLSSGRATSSMEFSHYEQ
                     VSQNMAKEILEKVKGKSAAN"
     gene            complement(15932..16408)
                     /gene="rpsG"
                     /locus_tag="PK252_09350"
     CDS             complement(15932..16408)
                     /gene="rpsG"
                     /locus_tag="PK252_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT14951.1"
                     /translation="MRKAKPKKRILLPDPKFGDTLVTRFVNSLMSDGKKNIAYKVFYQ
                     AIDAVAEKNKDAEKSPLEIWRKALENITPQVEVKSRRIGGATFQVPQEVRPDRKIALA
                     IKHMILFARKRSGKSMAEKLSAEILAGYNNEGGAFKKKEDMHKMAEANKAFAFFRF"
     gene            complement(16439..16843)
                     /gene="rpsL"
                     /locus_tag="PK252_09355"
     CDS             complement(16439..16843)
                     /gene="rpsL"
                     /locus_tag="PK252_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT14952.1"
                     /translation="MPTIQQLVRKGRKKLEDKSKAAALSSCPQKRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTHGIEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHLVRGTL
                     DASGVEGRKRSRSLYGAKRPKPGAAAAPAKKK"
     gene            complement(17127..17939)
                     /locus_tag="PK252_09360"
     CDS             complement(17127..17939)
                     /locus_tag="PK252_09360"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT14953.1"
                     /translation="MLETINQTVSFICQKTKSKPKIGIILGTGLSGLANEIKQDVVIS
                     YKDIPNFPVSTVESHPGRLIFGTLGKHQVVAMQGRFHYYEGYSTQQIAFPIRVLKALG
                     IEFLFLSNACGGLNPSFEVGDLMIIDDHINLIPNPLIGKHYEEFGPRFPDMSCPYDLQ
                     LINKAINIAKTLNLKIHKGCYVAVTGPSLETPKEYHFLRVIGGDAVGMSTVPEVITAR
                     QMNITCFAISIITDLGVPGKIKEVSLDEIIKASETAEPKMTAIFRELINDIE"
     gene            complement(17884..19005)
                     /gene="lpxK"
                     /locus_tag="PK252_09365"
     CDS             complement(17884..19005)
                     /gene="lpxK"
                     /locus_tag="PK252_09365"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOT14954.1"
                     /translation="MVQKLNLFAKFIAFPIAIIYGVIVWFRNLLFDFNILRSREFDIP
                     VISVGNITVGGTGKTPHVEYLVELLGAEFEVATLSRGYKRKTIGFRLVNPESTPVEAG
                     DEPCQIKRKFENITVAVDAIRVRGIEKLQQLNPLLKVVILDDAYQHRYVKPGINILLV
                     DYNRPIYEDQIIPLGRLREFAGDRDRADYIIVTKCPDTIKPIDQRLIEKRLKLYAYQT
                     VLFTKMVYKEPKPVFPALKPAFTIDSFEKDKFAAVVVTAVATNTSYIEFLEKKFNIIK
                     VLAYPDHYMFKATDIHNLIEIYCNIDHPKKVIITTEKDAVRFTTFENLEDEVKSAMYY
                     YPIGVEFLHGAEKMFNQEMISYVRNNKPDSLLHLSKNKK"
     gene            19575..20441
                     /locus_tag="PK252_09370"
     CDS             19575..20441
                     /locus_tag="PK252_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT14955.1"
                     /translation="MKLLHKATEDLASLSRGNLNIELDPDALNLNDEVGKINRSMELM
                     QENFRNVIYSVKSSSETLVNEAKNLNKASAILTEAANDQAASIEEISASMEEISANIQ
                     HSANNAKETEVRSKKASDTVEKVGITSQKSQKAVQNISQKISVINDIAFQTNILALNA
                     AVEAARAGEHGKGFAVVAAEVRKLAERSKAAADEIVQIVQTTVNETGAANALVEELLP
                     EIKDTSRLVQEITASSIEQSSGAEQINNSIQQLNNRTQQNIANVDTMNNSAHRLNESA
                     EQLEKAIKFFKI"
     gene            20473..20766
                     /locus_tag="PK252_09375"
     CDS             20473..20766
                     /locus_tag="PK252_09375"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT14956.1"
                     /translation="MKLDDKKIETIKGYFKARPVLKAYLFGSYVRGDADEKSDIDILV
                     DLDYSQKIGLQFIQMKIDLEQLLSAKVDLVSENGLSKYIKPIVDKEKLLIYAK"
     gene            20830..21090
                     /locus_tag="PK252_09380"
     CDS             20830..21090
                     /locus_tag="PK252_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT14957.1"
                     /translation="MGADFDKFMNDSMMRFACIKQMEIIGEASNHVSEEIKQQFSEIE
                     WDQIVGMRNVFVHEYFGVDSTLIKSDLPELKEKVKEILNSIE"
     gene            complement(21317..24415)
                     /locus_tag="PK252_09385"
     CDS             complement(21317..24415)
                     /locus_tag="PK252_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT14958.1"
                     /translation="MKTKTTLLVLFVLWISHFSANAQIIECSPAFPKVTDSVTITFHA
                     DMGSKGLMDYTGDVYAHTGVLISSSSSPGDWKHAPTWPTNTAKYKLTKIADNTYQLKI
                     KPSISEYYGVAQSETVTKLAFVFRNADGSKTGKGNGETDIFYDVFSVAGLKMVVLNPL
                     SKINVGKINDSFEVKVITSEADSTVVFKDGTPVLTNKTERFTYTVTISKTGVSKIVVR
                     AYKGVETLSDTLSFYCAESTKVKDLPAGIVNGVNLISESSVLLTLTAPGKDKVTVIGD
                     FNNWIPDTTYQMFKTSDGNKFWVQIDNLEAGKEYAYQFVVDSLVVADPYCHKVLDPDN
                     DKGITFAVYPNLKAYPLGKTTGIVSVLQTKPAEYNWKYNSNPMPESQKLVIYELLVRD
                     FAAQHSYNAVIDSLNYLKTLGINAIELMPVMEFEGNSSWGYNPSYHMALDKYYGTPEA
                     LKKLIDTCHANGIAVILDVVLNHAFGQNPMVQMYWDANNSRPAANSPWFNPVAKHDFN
                     VGYDFNHESAYTKQYVADVLRYWLTEYNIDGYRFDLSKGFTQKNTLGNTSAWGTKDDS
                     RIAILKNISDTIRKYKQDAYIILEHFANNDEEQVLADYGFMLWANMNQQYRDAAMGWT
                     ESGKANFTGVSYLSRSMTKPALVAYMESHDEERIGYSNPTWGNTSNPLYATRKLPVAC
                     QRAELGAAFFFTIPGPKMVWQFGELNYDYKIEYNGRTGEKPIKWDYYYNPNRQRLYEA
                     YAALIALKTKLNAFNSSNYTLNLAAKVKTIAIDDTVVKVRIIGNFDLYAQAGVLTFPS
                     TGYWYNYMTGDSINVTSAETTFNLQAGEYLILTNIRLKNPNIIDAPKAMDVNIAGDIM
                     VKKSATESTDQDLVAFYTYFDANKDVEGASKYQWYRSTASTGLNMVAIDGATSLNYNV
                     TTDDFGYYLIFEVTPVAATGFIFEGLPQYATIGIRTAINSVDATKISIYPNPASDYIN
                     ISSGSPIKSIDIYDINGRIVLKSVNIVDSESVNVSNLKSGLYFVNAYTSKGTIKAKFL
                     KK"
     gene            complement(24541..25518)
                     /locus_tag="PK252_09390"
     CDS             complement(24541..25518)
                     /locus_tag="PK252_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT14959.1"
                     /translation="MKKHVLLLMIAALMIKGLTIAQNAKITFIADMTYMIKAGKFDKT
                     NDFVDIAGTLNGWDGSNSHLAPMPGNDSLYAITIDTFTAGTVVEYKFRINGSWDNDKH
                     EFPSGGPNRKITVRKDSIGVKKIFNNYRPGYVPVQISVNMSVAKEKGKFDPETNFLDV
                     AGTFNDWGASPDELFGTEDVYSATILCPIGEIQFKCRIDGSWDDAKHEFPAGGAARTY
                     NVFDTTGVKSTNVIPVFYFNNEKPAQTSISTVSANMACYPNPCYDVLNIKAVNEVNSI
                     AIFDITGKQLINYYGNIKSVNVSGLNAGTYIVKISESSGKAFTQRIVKK"
     gene            25746..26780
                     /locus_tag="PK252_09395"
     CDS             25746..26780
                     /locus_tag="PK252_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT14960.1"
                     /translation="MRTAQTTIKDIASRLGLSPSTVSRALKDHPDIGADTKKAVKDLA
                     VELNYKPNAMAISLRNSRSNIIGVIIPEIVHHFFSSVISGIEDVAYDAGYQVMVCQSN
                     ELYQREVKNTQALLLSRIEGLLVSVSKETRDFSHLRGVKELNIPVVFFDRVCDEMEAD
                     RVIVDDFEGAYKIVSHLIEVGCKRIAHLGTVQDLLIGRNRLNGYLKALKDHNLPIDDK
                     LILRCDTMQSAQIATKRLVYELNPPDGIFAVNDLTAIGALQTIKECKMRVPDDICVAG
                     FGNGLYSMFTDPPITTVEQNGYKMGQVAMTLMLDRLIRHDDYPPITEVLETSLIQRKS
                     TQKPIKPQYI"
     gene            26806..27765
                     /locus_tag="PK252_09400"
     CDS             26806..27765
                     /locus_tag="PK252_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT14961.1"
                     /translation="MKILVIMNANNYQPTSINNKMKKSKKYLPYILLAFGLLCIGWSA
                     VFVKLADVSGLVSGFYRMFIGAISIMPLWLLRHKSWKIDAGSFRIAVLCGVLFACDIA
                     LWNTSIMLSKAAIATLLANMAPVWVGLGSYFILKEKAGRNFWIGCIISIIGVSLITGI
                     TSISASMFSWGAILAIMASFFYAAYLLITPRVRGKVDTISFTAVSMIASSVVLLIFCM
                     VSESKLTGYSTKSWMALAGLGFVSQFLGWIAINWALAYINSTTASVTLLSQSIVTAII
                     AIPVLGETLSFIEIIGAIVVLTGIFLVIKKQKASKTEILPEYE"
     gene            27758..29107
                     /locus_tag="PK252_09405"
     CDS             27758..29107
                     /locus_tag="PK252_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14962.1"
                     /translation="MNDTTQVKMPLNPLKGTWTNASLSIGRVFTIFIKKSSLFSDILK
                     VKIYLLLIIAVTICSCRPTFKVRKLSYDDSTGFYKYDKSVPVILPDKRLVNKDNIQAF
                     IQFIASYRYADNNNLLYQPDWVVSKKDKELLDSYNKVIQNILNKEYQISLSEAKTFKS
                     KLNGAGGFSDIDFWIAYAHLNLSNNDSAAYYFTQFLLYSGYKYPIKFRGHFNSEHATR
                     LLAEQKKYAADFLEGREDLPPVKPEIVKHRYYYQSHTPGFIINHEDAGVKNFNFFVLP
                     ILYDNNFQGGQGGFSYNKFKTLNFSVYSEIEKEQQSLKIRIPWQTYISATHRLGIKIT
                     PQCRFYSSVINDENYKGILTGSSVSIGYRITHRVYAGIAYTTLYSTHDAFINKRFYRA
                     QQLHGSYDAGIHLQLIKSFSLKLGYSDGYGMFGLLINNVFSGYNEKTKSSMFQFNDF"
     gene            29155..>29445
                     /locus_tag="PK252_09410"
     CDS             29155..>29445
                     /locus_tag="PK252_09410"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOT14963.1"
                     /translation="MWKKIGAITLVLFFSYIVNAQNADDICGYWRTLKGNTQFHITKN
                     ENGLYSITIVWLRIKKDRTDANNPNPNLRNRKFLQLVISDNIVFLKDKNKWVG"
BASE COUNT         8796 a         5950 c         5027 g         9672 t
ORIGIN      
        1 atttcaaaaa ctaacttttc atagtctgtt ttctgctcta cacgatagtt ttctatagca
       61 tattttacat tcttaatagg agtaaaaatt gaatctattg ctattaaacc tatttcacta
      121 tttacaggtt tattctcctc agatgctaca tacccacggc ctttattaat gttaagttcc
      181 atttgcaact taacagaagg gtctaaatga caaataactt gttctgtatt aagaacttga
      241 aaaccattta aaaatttatt aagatcccca gccttgaaaa cactgccacc agcaaagttt
      301 acatgaatct tttcatgatc aacatcttca acaacccttt tgaaccttac ttgtttcagg
      361 ttcaaaataa tctcggtcac atcttcaatg acgcccttaa ttgtggaaaa ttcatgatca
      421 acaccctcaa tttttattga agtgattgca aaaccttcca aagaagaaag taatattctt
      481 cttaatgcgt taccaatagt gataccataa ccgggctcga gtggcctaaa ttcaaattgc
      541 cccgatgttt cggtagcact caccatgata accctgtcgg gtttttgaaa tgctaatatt
      601 gccatatata atgttatttc gagtacaatt ctacaataag ttgttcctta atattttcag
      661 gaatatcact gcgttgtgga acgttcatat atttcccgct aaaggtgtct ttattccact
      721 ctaaccatga aaacctgtta tatctggcaa ctttaagcgc atcattgatt acttccaaag
      781 attttgattt ttctcttaca gaaataacat ctccaggttt aactatatat gaaggaatat
      841 taacaatttc cccatttaca gtaatatgtt tgtgtaaaac aagctgacgt gccccattac
      901 gagtattagc caaactcaaa cgataaacta cattatcgag ccttgactca agtaattgta
      961 ataacacttc gcctgtaaca cctttactcg cttgtgcctt aacaaacaag tttgaaaact
     1021 gacgttctaa aacaccataa gtgtatttgg ctttttgttt ttcacgtaat tgcaacccat
     1081 actcagaaac ctttttgcgt ttacgagcta aaccatgttg tcctggagga taattacgtt
     1141 tttcaagatt tttatctggt ccaaaaatag gctccccaaa tattctggct atttttgtct
     1201 ttggtcctgt atatcttgcc attgtattgt aaatatatta tgcaataatt attaaagaaa
     1261 ttaaactctt ctacggcttg gaggacgaca tccattgtga ggcataggtg ttacatcaac
     1321 aatttctgta acctcaatac cacaagcatg tattgaacga atcgcagatt ctcgcccggc
     1381 acccggacct ttaacaaaca cttttacacg tcttaaacct gcatcataag cagttttacc
     1441 acatgtttca gctgctgttt gagctgcata aggagtgttc ttttttgaac ctttgaaccc
     1501 cattttcccg gctgatgacc atgcaataac ttgtccttca ttattggtca atgaaattat
     1561 aatgttgttg aaggacgcat gtatatgagc ctgaccggtt gactctactt taacaacttt
     1621 ctttttggat gttcccgtct tttttgccat gtcaaattag ttattatcct ttagtagctt
     1681 ttttcttatt agcaattgtt ttctttttac cttttctggt tcgagcattg tttttggttc
     1741 gttgcccatt aacaggtaat cctacccggt ggcgaagccc acgataacaa ccaatatcca
     1801 ttaaacgttt gatatttaat tgaaccatcg aacgaagctc tccttcaact ttatactgtt
     1861 cgttgataat actacgaatt tttgaaagct gatcatcatt ccagtctttc acttttgtat
     1921 cttcatcaat tccagcttga gacaaaatgt ccttcgcaga actaacccca ataccataaa
     1981 tataggtcaa ccctattact cctcgtttgt ttttaggtaa atcgacacct gcaattcttg
     2041 ccatattcta tttttttgga agtgcaaaat taataattta tccttgacgc tgcttaaatt
     2101 ttggattttt tttgttaata acataaagtc tccctttacg cctaacaatt ttacagtctt
     2161 cgctcctttt tttaattgat gttctaactt tcatatatca gttattttac ttatatctaa
     2221 atgaaattct tccttttgat aaatcgtatg gagacatttc aacttttact ttatccccag
     2281 gcaaaagttt tatataatgc atcctcattt tcccggaaat atgtgcggta ataatgtgcc
     2341 cattttctaa ctctacccgg aacattgcat tggacaatgc ttcggtaata gtaccatctt
     2401 gctctataga tgcttgtttt gacatattaa aactccttat ctcctaaaac ttcttctata
     2461 tatttaaaag ttcctaaaat atctcctttt tctttttcaa tagcaacagc taattcaaaa
     2521 tgtgctgaag gtaagttgtc ccaagtccta attgtccatc catctctttc ctggtgaatt
     2581 tttcttgttc ccatgtttat cattggttca atacaaatta ccattcctgt tttcatcatt
     2641 ggtccagttc ctttcttgcc ataatttggc acctcaggtg cttcatgaag ttttcttcca
     2701 attccatgac caaccatttc tctaactaca gaaaaaccag cattctcgca ataagtttga
     2761 accttaaacc caatatcacc aattctctta ccttctcttg cttctgaaat accttcaaat
     2821 aaactatttt tggtaacctt aagcaaatgc tttacacgct cagtaatttc tccaactgca
     2881 aaagtgtatg cagaatctcc ataatattcg tttaaaacaa ctccacaatc aaccgaaata
     2941 atgtctcctt cgttgagtat tgttttatca tcaggaatcc catgtacaac cgcgctattg
     3001 agactagtgc ataaagtttt tggaaatcct ccatacccta aaaaagcagg aataccacca
     3061 tggtccctga tatactcttc tgcaatctta tcaagataat ttgttgaaac cccggctttg
     3121 atatattttg caagctcacc taaggtcttg gcaactaatt cgttacttgc cctgagtttt
     3181 tcaatctctt cttgtgtgcg tattgctatc atcaaataaa gaactctcgc tttttcagat
     3241 cttatattcc cgatctgcct ttgatccggc cggttttcat aaggccatca taatgtctca
     3301 tcaacagatg actttctatt tgttgtaaag tgtctaaaat aacacctacc ataattaaca
     3361 aactggttcc accataaaaa tgcgcaaatc cacttgtcat acctgctttc attgcaaatg
     3421 caggaagaat agcaactata gctaaaaata tagctcctgg taaagtgatt tttgacatta
     3481 cactatcaat aaattcggct gtctttttac caggtttaac acctggaatg aaacctccgt
     3541 ttcttttcat atcttcagcc atctggttag ggttcacagt aattgcagta taaaaataag
     3601 tgaataccaa aattaataac ccaaatgtaa aattgtacca aaatccggta taatttgcaa
     3661 aagctgctac aaaaccagaa gctttttctg aatttgcaaa gccagctact gtaataggta
     3721 taaacattaa tgcctgagca aatataatag gcattacccc tgcagcattt actttcaaag
     3781 gaatatattg acgaactcct ccgtattgtt tatttccaat gatacgtttg gcatattgca
     3841 caggaatttt tcttgtccct tgtattaaca aaattgttcc ggcaaaaaca acaaacagta
     3901 taatgatttc aagtaataat actaccaacc cgccaccttt ttgatcaaga cgtgaagcaa
     3961 attcttgcaa aaatgattct ggcaaccggg caataatacc caccataatc aacatggaaa
     4021 taccgttgcc aataccattt tctgtaatac gctcccctaa ccacatgata aaaattgttc
     4081 cggcagctaa caggattact ccagtaatat caaacatccg tcctccgtaa attgccccct
     4141 gatattggac atgtaggtta gttaaatagg ccggtgcttg caacaataat ataataactg
     4201 ttaaaagcct tgtgtgctgg ttcattttac gacgcccact ttcaccctct ttttgcatac
     4261 gctgataata aggaacagcc attcccaata actgcatcac aatagaagca gaaatataag
     4321 gcataacccc aagcgcaaaa atagaagcat tagaaaaagc cccacccgaa aacatgtcaa
     4381 gcaaacccat taccccttga gctgtttgtc ttttaagatc acttaacatt acagggtcta
     4441 taccagggag aactataaat gaacctaaac gataaatcaa aataattcct aaagtatata
     4501 atatcctagc tttaagatcc tctattttcc aaatattctt cagcgtatca ataagttgtt
     4561 tcattgagct tcaattttaa caattgtacc attagcagct tttattgctt cttcagctga
     4621 tttagagaaa gcatgagcag taacttcaac ttttgctttt aattctccct tgcctaaaat
     4681 tttcactaaa ctgtttttat ttatcaatcc tttttctagt aataactcag gagaaataac
     4741 agtcaaacct gcctctgcta atttttgaat aacctcaagg ttaatcgctt taaactcaat
     4801 tctgttgggg ttagtaaacc caaacttagg taaacgccgt tgcataggca tttgtccccc
     4861 ttcaaaacct ctttttcgag aatatcctga acgagactta gctcctttat gccctctgcc
     4921 ggcagttcct ccatcagttg atccttgtcc tcgacctaac cttttgtttg tcctaacaga
     4981 accttgtgct ggctttaaat tacttaaatt cataatttta tgtatatgct cttattcaga
     5041 taattatact tcttctactt ttactaaatg atgaaccttt gatatcattc ctttgaaaac
     5101 atcatttgac tcaactacga cactgctggt acgacgtcct aatcctaaag ctttaatagt
     5161 tcttagttgt ctttcgcaaa ccctgctttt actttttacc aaggtaactt ttaatttgct
     5221 catagtatat tttattatcc tttaaatact ttttcaacag atatacctct ggaagctgca
     5281 actgccattg gatcacgcaa ttccaacaaa gcctgaaatg tagcctttac aaggttgtga
     5341 gggtttgatg acccttttgc cttagcaagt acatcatgaa tgccaacact ttctagaact
     5401 gcacgcattg caccaccagc ttttactcca gtaccacttg aagcaggctt aataaacaca
     5461 gatgctccac caaatttggc aaattgttca tggggtattg tccctttata aataggtact
     5521 tttattaagt ttttcttggc atcttcaatt cctttcgaaa ttgcagtagt tacctcattt
     5581 gctttaccaa gcccatagcc aacaattcca ttttcattac ctacaacaac tattgcagaa
     5641 aaactaaatg cacgtccacc ttttgtaact ttggtaacac gttggatgct tactaacctg
     5701 tcttttaatt caagatcact agtttttatt ttgcgtatgc tttctgacat aattctagaa
     5761 ttttaaacca ccttcacggg cagcatctgc taatactttt actctaccat ggtataaata
     5821 gccatttctg tcaaatacaa cttcggctat tcctttttgc ttacataatt cggcaatctt
     5881 tttgcctact aattgagcct gatcaatctt tttgcctttt gaagcctcag caatttcttt
     5941 ttgcagcgac gaagcagcta aaagtgtaat accctgacta tcatcaatgc attgaacata
     6001 aatttgttta ttgctcctaa aaacagtcat cctaggcttt tcggcagtcc cgaaaacaac
     6061 tttacgaatg cgatgtttta ttctggctct tctttcttgt ttcgtcaaag acatatctta
     6121 aaattttact tatttaccag cagttttccc ggccttacgc ctaatatttt cattaacaaa
     6181 cttaatacct ttacctttat atggttctgg tttacgtaat gaacgaatct tggcagcgac
     6241 tacacctaat aattgtttat cagcacattg cagagtgata ataggatttg ccctcttttc
     6301 tgtacgagct tcagctttga tttctttagg caataccatc aaaatttcat gagaatatcc
     6361 taaactcatt tcaagatgtt gaccttttac ttcagcccta taaccaacgc caaccaactc
     6421 ctgctcaact ttgaaacctt ctgaaactcc ttttatcata ttagccaata acgcacgata
     6481 taacccatgc atagctttat gccttttttg atcagtcggg cgagttacaa caacctgatt
     6541 ttcaacaact tcaactttaa tatcagcatc tacttgttgt tttaactctc caagtggccc
     6601 ttttacggta acttcattgg atgggctaac attgatagcc acaccttttg gaagatttat
     6661 tggtaatttt cctatacgag acatattata ccagtattaa taaacgtaac acaaaacttc
     6721 gcctccaatg ttccttaacc gagcttcttt atcggtcaac acgccttgag atgttgaaac
     6781 gatagcaata cccaaaccat tcaaaacgcg tggcatatct tcacaacttt tatagttacg
     6841 taaaccggga cggctgacac gcttaataca tttaatggct gaagatttgt ctgcagggtt
     6901 gtatttcagt gctattttta taacaccttg aggaccatta tcttcgaatt tataattcaa
     6961 aatatacccc ttatcaaaaa gtatttttgt tatctctttt ttcaagtttg aagcaggtat
     7021 ttcgacaata cgatgctttg ctctgatggc atttcttata cgagtaagaa aatctgctat
     7081 tgaatctgtc attttattat aataatgatt tacaaattac caacttgctt ttttaactcc
     7141 aggaatcaaa cctttggaag ccatttcacg gaattgaata cggcttattc caaattgacg
     7201 catataacca cgtggccttc ctgtcaacat gcatcgattg tgaacacgga ttggatttga
     7261 atttggtggc aatagttgta atccaacaaa gtctccttct gcttttaact gtgctctttt
     7321 tgcggcatat ttttctacga gtttcattct tctcttctcg cgagctttca ttgattcttt
     7381 tgccataact tatttctttt ttgcagattt aaaaggaatt ccaaattcac gtaataatgc
     7441 aaggccttct tcatcgcttg ttgctgttgt aacaaagctg atttcaagtc ccatcatttt
     7501 tgtaatcttg tcaatatcaa tttcaggaaa aataatttgc tcagtcaaac caagtgtata
     7561 attcccccga ccgtcaaact tagcattaac ccctttaaag tcacgaatac gaggtaaagc
     7621 aactgcaata agacgttcca gaaattcata catccgatcg ccacgcaagg taacttttgc
     7681 tccaataggc attttactac gaagcttaaa gtttgaaata tctttctttg caacagtttg
     7741 aactgctttt tgtcctgaaa tttgagtaag ctcattcaaa gcaacttcaa taagcttttt
     7801 atcagccaca gcagctccaa ccccttggtt aataacaatt ttgttcaagc gtggaacttg
     7861 cattacacta gtgtaattga attccttctt taatgcagga acaatatcgt ttgtatactt
     7921 tgtctttaag tttggaatgt atttcattat tttatttcct ccccagattt tttagcatat
     7981 ctaaccaatt tcccttcagc gtttaaacga cggcctatac gtgtaggcac actgctcttc
     8041 ggatcaataa gcattaaatt tgaaatatga accggagctt cttgcttaac aatcccacct
     8101 tgtgggtttt tagcattagg cttggtatgc tttgaaacca tattgatact ttcaacaatt
     8161 gcagtttctt tttcgcggtt tacttctagt attctaccgc ttcttccttt atcgtctcct
     8221 gcaataactt ttactatatc accctttttt atatgcaact ttttttgcat cgctttgtgt
     8281 ttttaaaatt aaagcacttc tggtgccaga gaaacaattt tcatatattt atcacgtagt
     8341 tcacgagcta ccggaccaaa aatacgtgtg cctctaattt catcagcatt attcaacaat
     8401 accacagcat tgtcatcaaa gcgaatgtat gaaccgtcag cccgacgtat ttcttttttt
     8461 gtccgtacaa taacggcctt gctaacgctg ccttttttta cttctcctga aggcaaagcg
     8521 cttttaatac taactactat tttatcgcca acgctggcat accttctgcg ggtgcctcca
     8581 agtaccctga tgcagagcac ttctttggct ccactgttat cggcaacagt cagtcgagat
     8641 tcttgttgta tcatgactat ttaactttct caattatttc tactaatctc catctttttg
     8701 atttggacaa aggccttgtt tccataatac gtacagtatc tccaatactg catatattat
     8761 tatcatcttg tgcgtaaaat tttgaggttt tattcacaaa tttatgataa atagggtgtt
     8821 tcattttacg ctttacggat actactatgg tcttatccat tttgttgcta actacaaccc
     8881 caatacgttc ttttcgtaga tttctttctt ccatcgtatt gaactatttc ttaatatcgc
     8941 ttaattctct catggataac tctgtggcca tacgggctat gtcacgacga gtatgcctaa
     9001 ttttcattgg gttttccaat ggtgtaactg catgattgat tctaagcttg gttaacatgc
     9061 ttttttctgc atcaattctt tcttgcagtt cttttgtgct taattctctg atttcggatg
     9121 ttttcattat ctatcagatt gtttcggagt aatctctcct ggttataaat ttagtactaa
     9181 caggtaattt ttgagctcca agtcgcaatg cttctttagc aacttcttgt gaaacgcctt
     9241 caacctcaat aataatgcgc cctggtgtaa taggatatac aaatccttcc ggggctcctt
     9301 tacctttacc catacgaact tcagcaggtt tctttgtgat aggcttgtca gggaaaatcc
     9361 taatccaaat ttgaccttca cgtttcatat aacggcttac tgcctgacgg gcagcttcta
     9421 attgccggcc attaatccaa caactctctt cagttttgat tgcaaaagac ccaaaggcta
     9481 tggtgttccc acgttgggca ttgcctttca tacgtccttt ttgttgcctt ctatattttg
     9541 ttttctttgg ctgtaacatt gttcaaatat gattatatat tgttactttc tttttctttt
     9601 actttctcct gattgacttt ttatagcctt aggagctttg ctttgaatgt tgggggacaa
     9661 atcacgtttc ccataaactt ccccattgca tatccaaact ttgatcccca aacagcctac
     9721 tttagtatga gcttcggcta atgcatagtc tatatcagca cgaaaagtat gcaaagggat
     9781 tcgaccttct ttgatcattt cggtacgagc catttctgcc cctcctaaac gcccggctat
     9841 catcactttt ataccttcag cccccatacg catagtagaa gctattgcca ttttgatggc
     9901 tctacgatat gatacacggc cttcaacctg acgtgcaata ttataagcta caatatttgc
     9961 atctaactca gggcgtttta cttcgaagat gttaatctga acctctttgt tggtaatttt
    10021 tttcaactct tctttcagtt tatcaacttc ctgtccgcct ttcccaataa taataccagg
    10081 acgagcagta ttaacagtaa ttgtgataag ctttaaagta cgttctatga taatcttaga
    10141 aatacttgct ttggctaatc gggtatttaa atattttcta atttcagcat cttcaacaag
    10201 tttagctgaa taattctttc caccgtacca attagaatcc catcctttga tgattcctaa
    10261 cctatttgat attggattta ctttctgtcc catttactta ttttgtttga cttgcaacat
    10321 tagccgattc taaaactaaa gtaacatgat tagatctttt tctaactctg tatccccttc
    10381 cctgaggagc agggcgtaat ctcttaagca tacgtccacc atcaacaaaa gctgttttaa
    10441 caagcaaatt tgcatcttca aggcgtaccc cttcattttt agcttgccaa ttagcaatgg
    10501 ctgataataa aagcttttct actttctttg aagcttcttt tggtgaatat ttaagtatat
    10561 gtaatgcttt attcacttca acccccctga taagatctac aactaacctc attttgcgag
    10621 gtgaacttgg acaattattt aatactgcaa tagccttatt ccttttagct tcttttaagc
    10681 tttcggccat cttacgttta cgagttccca tttgaaagta tcgatttaca aatgtttaaa
    10741 ttattctttt ctgttaccac tgtgaccacg gaaggtacgt gttggggcaa attcgcctaa
    10801 tttatgtcca accatgtttt cagttatata caccgggata aatttattcc cattgtgtac
    10861 agcaatagta tgccctacaa agtctggtga tattactgaa gctcgggccc atgttttaac
    10921 aacactcttt ttattttcat tattcaaagc aagaactttt tgttcgagtt tgaaattaat
    10981 gtatggtcct ttttttaatg aacgactcat atttctgtat atgtattttt atactacttt
    11041 cttctttcaa taatatactt attggaatct ttgtttttcc aacgagtctt atatccttta
    11101 gctggtaagc cttttcttga acgtggatgt cctccggaag cccttccttc tccaccaccc
    11161 attgggtgat caacagggtt cataacaaca ccacggtttc ttggacgacg ccctaaccag
    11221 cgcttacgac ctgcttttcc taatttttga tttgaatggt cagaattaga aactgctcca
    11281 atagtagctt tacaagtaac aagtaccatg cgggtctcac ctgatggcaa ttttattatt
    11341 gcaaatttac catctcttga agtaagctgt gcgtaggaac cagcgcttct tgccatggca
    11401 ccaccttgtc ctggcctaag ttcaatgtta tgaataattg tacccagtgg aatatcactt
    11461 aatggaagtg catttcctaa ttctggctca acaccagctc cacttataac tttctgtcct
    11521 tctttaagcc cttcaggagc aagaatatat cttttttccc catctgcgta aactaacaat
    11581 gcaatacgcg cagtacggtt aggatcatac tctatgcctt ttacagtggc aacaatacca
    11641 tctttattac gggcgaagtc gatgatacga tacatctttt tgtttcctcc gccaatatag
    11701 cgcatggtca ttttcccggt attgtttcga ccaccgctgc tcttcacact agtcaacaaa
    11761 gatttttcag gagttgattt tgtaatctcc gtaaaatcta caagaatttt tcctctttga
    11821 ccaggtgtaa ccggtttaaa cttttttaat cccattgtct attttaaact atatattgct
    11881 aaagaaatct attttgtcat tattagctac agtaataata gcttttttgt atgaggaact
    11941 ccttccctgg ataaaaccgg ttttggtgta cctcgattta tttttgccac ctactattaa
    12001 agtattgaca gattcaactg aaacaccata catatcttca actgcttttt taatttgcag
    12061 cttgttggca ttttttttaa ccttaaaggc gtacatattt tgtttctcgc ctattttggt
    12121 cattttttca gttactatgg gcttaattaa aatatccatc ttttttcttt gttatgcatt
    12181 tgtttgcaac gcaccaattg ttttctcact taataccaaa attccagcat tgacaatatc
    12241 gtaagtgttt aattcagaaa cagttacaac ctttgcatcc tgtaaatttc gggacgacaa
    12301 atatacattt ttatttggtt cgctaagcac aagaagcatt tttttattgt caatttttaa
    12361 attacttctc atttttatga aatcttttgt tttgggaaca tcaaatgaaa agtcttccaa
    12421 aacaactaaa ctattttctt ttgctttgta agataaagca gaaaagcgag ccaaatcttt
    12481 tacttttttg ttcagtttga agccataagt acgaggacga ggcccgaata ctctacctcc
    12541 acctacaaaa accggagatt taatgctacc tgcacgtgca cctcctgtac ctttttgttt
    12601 tttgatcttt tttgtactat aggcaacttc agctctttct ttagctttat gtgttccttg
    12661 acgttgattt gcaaggtatt gtttcacatc aagataaatg gcatggtcat ttggctctat
    12721 agcaaaaatc tcgtctttga gctcaacgct acgaccagtg tcagcacctg atatattaac
    12781 tactgaaatt ttcattctct taaattttta tctagagtaa attttacttt tcaatgatga
    12841 cgtaaccacc ctttgcaccc ggaatagagc cttttattaa aagaaggtta ttttcaggaa
    12901 ttattttaac aatttgaagg ttaagcgctt taacattaac atttccagta tgtcctgcca
    12961 tacgtttacc tctccaaacc cgggacggga aagatgatgc tcccatagaa ccaggggccc
    13021 ttaaccggtt atgttgacca tgagtttgtc ctccaacccc gctgaaacca tgacgtttaa
    13081 caaccccttg aaaacctttg ccttttgatt gtcctttaat atcaacaaaa tcgttaactt
    13141 gaaacaaatc agtgctaatt acatctccca attttgcatc ttcaaatcct tcaaattcaa
    13201 caaccttacg ttttggggca gtatttgctt ttttgaaatg atttaaaagt gcttttgtag
    13261 tatgcttttc ttttttgtca tcataagcta actgaacagc ctggtagcca tctttttcta
    13321 cagtcttcac ttgtgttata acacaaggac cagcttcgac aactgttaca gggatttgtt
    13381 ttccttcttc tgagataaaa gaagtcatcc ctaccttttt accaattaat cctggcattg
    13441 ccgtacaaat tttatagtta tacttttatt tcaacttcaa ctccactagg cagctctaac
    13501 ttcatcaacg catcgatagt ttttggagta gaactataaa tatcgagcag acgtttgtat
    13561 gaactgagtt caaactgttc gcgtgatttt ttgttgacga atgttgaacg gtttacagta
    13621 aaaattcttt tgtgcgtagg taatggaatt gggccactga caactgcacc cgtagctttt
    13681 actgttttta caatcttatc ggcagattta tctaccagat tgtaatcgta agattttaat
    13741 ttaattctaa tcttctggct cattagtaat atattttaat ttgctgcaga tttacctttt
    13801 actttttcta gtatctcttt cgccatattt tgcgatactt gttcgtaatg tgagaactcc
    13861 atgctactgg ttgcacgccc tgaacttaag gtccgcaata ctgttacgta tccgaattgc
    13921 tcagcaagag gtactttagc tttgataacg cggccccctg ctttcatttc cataccttca
    13981 acattgccac ggcgtttgtt taagtcgcca ataacatcac ccatgtattc ttcaggtgta
    14041 acaacctcaa gtttcatgat aggttcaagt aagataggat ttgctttaga gcaggcttct
    14101 ttgaatgcca tttttgcaca aatttcaaaa gaaagagcat cagagtctac tgggtgatat
    14161 gaaccatcga gcaaagttac ttttaagcta tccattgtat atccggctaa cacgccatta
    14221 cgcatagctt ctttgaaacc tttttcaaca gatgggatat attctttagg gatgttgcca
    14281 cctttaactt cattaataaa ttggaggcca acaactcctg gatctgcagg ttcaacttta
    14341 acaattatat cagcaaactt accacgtcca ccactttgtt ttttgaatac ttcgcggtgt
    14401 tgaacacttc cattgatagc ttctttataa gcaacttgtg gagcgccttg gttgcattct
    14461 attttaaatt cgcgttttaa acggtcgatg ataatctcaa gatgtagttc acccattcca
    14521 ctgataacag tttgcccaga gtcttcgtca gtatttacac ggaaggttgg atcttcttcg
    14581 gccaattttg caagagccat gcccatctta tcaaggtcat tttgagtttt tggctcaaca
    14641 gcaataccga taactggttc ggggaaagtc attgattcaa gagcaatagg agctttttcg
    14701 tcacaaagcg tatctccggt acggatttct ttaaaaccaa caaccgcaca aatatctcct
    14761 gcttcaatta tatcgcgtgg attttgtttg tttacgtgca tttgaaaaag ccgagagata
    14821 cgttcttttt ttcctgtcct gctgttcaaa acataggaac ctgcagtaat tgtaccagaa
    14881 tatacacgaa catagcaaag acgaccaaca aaaggatcgg tagcaatctt aaaggccaaa
    14941 gcagaaagag gttctttctc atcgggtgta cgtacaacta tttcgtcagt ttcaggattt
    15001 tttccttcaa tattaggcac atcaagagga ctaggaagat atgcagcaac tgcatcaagt
    15061 aattgctgaa cacctttatt tttaaatgaa gccccacaca taataggggt aatcttcatt
    15121 gcaatagtag ctttacgagc aacacctttt aattcttcta ctgtaagagc atctttattt
    15181 tcaaagaatt tttcgagcag tgcatcatcc atttcgcaga tagcttcgag aagcttatca
    15241 cgatattctt caactgtttc ttttagatca gccgggacat caacaatatt gaatttttca
    15301 cctttagggt cagctgggtc atagatgtgt gctttatttt caaaaaggtc aacaacacca
    15361 ataaatttat cttcagcccc aataggaatt tgaaaaggca cagcatttgc tccgagacga
    15421 tcttttactt gctgaaccac ttcaaaaaaa tcggcacccg tacggtccat tttattgaca
    15481 aaaccgatac ggggtacatt gtatttatta gcttggcgcc aaacagtttc agattgaggt
    15541 tcaactcctc ctactgcaca aaaaagagca acagctccgt cgagaacacg caatgaacgc
    15601 tctacttcaa cagtaaagtc aacgtgaccc ggagtgtcta taatatttat tttatattcc
    15661 tggttattgt acttccaaaa acaagttgta gctgctgagg taatagtaat acctctttct
    15721 tgctcctgca ccatccagtc cattgtagca gctccatcat gaacctcgcc aattttataa
    15781 ttaacaccgg tataatacaa aattcgttct gttgttgtag ttttaccagc atcaatgtga
    15841 gccatgatgc ctatgtttct ggtatatgaa aggtctcttg ccattaaaaa atttttacat
    15901 aaaaatttac actaactcta cactatacat tttaaaacct gaagaatgca aaagctttat
    15961 tagcttcagc cattttgtgc atatcttctt tctttttgaa tgcaccacct tcattattgt
    16021 aacctgcaag aatttctgca gaaagttttt cggccattga tttgccactt cttttgcgag
    16081 cgaacaaaat catatgttta atagctaaag ctatctttct gtcaggtctg acttcttgtg
    16141 gtacttgaaa agtagccccc ccaatacggc gacttttgac ttctacctgt ggggtaatat
    16201 tttccaatgc ttttctccag atttcgagtg gagatttttc tgcatcttta tttttttcag
    16261 ctacggcatc aatagcttga taaaatactt tataagctat atttttctta ccgtcactca
    16321 ttaaactgtt aacaaacctg gtaaccagtg tgtcgccaaa ttttgggtca ggcaacaaaa
    16381 ttcttttttt tggttttgct tttctcatcg gtcagcaaaa attatctgtt aattattatt
    16441 atttcttttt agcaggagct gcagcagcac ctggttttgg acgtttagca ccataaagcg
    16501 aacgacttct tttgcgacct tcaacaccgc ttgcgtcaag tgtcccacga accaggtggt
    16561 agcgaacacc agggaggtct ttaacacgac caccacgaac aagtactatt gagtgttctt
    16621 gcaaattgtg tccttcgcca ggaatatatg cagtaacttc aattccgtgt gttaacctca
    16681 cacgggcaac tttacgcatt gctgagtttg gttttttagg agttgtagta taaacacgtg
    16741 tacaaactcc acgtttttgt gggcaactac ttagtgcagc agccttgctc ttgtcctcaa
    16801 gtttctttct tccttttcga actaactgtt gtattgttgg catacaatca agtatttaca
    16861 aataaattgt taataaaatg ggttgcaaag gtagattttt ttttctaata tcaaatatct
    16921 accactatat attttttatt tttttctaaa tttctttttt agcattagta taaagatgtt
    16981 ttttgtatca tacaaaaatc agattgtcaa ctttttgtat agctgagaag cttaatttct
    17041 gctcagacaa aacaaggtta tttttctgct gaaaaaggca gcaaatttaa aactctttcc
    17101 ttgaatgaga aaaaaaaaga aggattttat tcaatatcat taattaattc gcgaaaaatg
    17161 gcagtcatct taggttcagc ggtttctgaa gctttaatga tttcgtcgag gctcacttct
    17221 ttgatttttc cgggaactcc cagatcggta ataatagaaa ttgcaaagca agtgatattc
    17281 atctgacgag cggttatcac ctcaggtaca gtggacatcc caactgcatc gcctccgata
    17341 acacgcaaaa aatgatattc tttgggagtt tcgagagaag ggcccgtaac tgcaacataa
    17401 caacctttgt gaattttgag gttcaatgtt ttggcaatat ttatggcttt atttattagt
    17461 tgtaagtcat aagggcaact catatcaggg aaacgtgggc caaactcttc ataatgtttg
    17521 cctataaggg gattgggaat aaggttgatg tgatcatcaa taatcataag gtcgccaact
    17581 tcaaaagaag ggttaaggcc gccacaggca ttggataaga aaagaaactc gatacctaaa
    17641 gcttttaaaa cccttattgg aaaagctatt tgttgggtcg aatacccttc gtaatagtgg
    17701 aaacggcctt gcatagcaac aacctgatgc ttgccaagcg tcccaaaaat gagtctacca
    17761 ggatggcttt caacagtaga aacaggaaaa ttggggatat ctttataaga tataacaaca
    17821 tcctgtttga tttcattggc aaggccagat aaaccagtgc caaggataat gccaattttg
    17881 ggcttacttt ttgttttttg acaaatgaag gagactgtct ggtttattgt ttctaacata
    17941 cgaaatcatt tcctggttaa acattttttc ggctccatgt aggaattcta ccccaatagg
    18001 ataataatac atagcacttt tcacttcatc ttcaagattt tcaaaagtag taaaacgcac
    18061 agcatccttt tcggtagtaa tgataacctt tttagggtgg tcgatattgc agtaaatttc
    18121 aattaaattg tgaatgtcgg ttgctttgaa catataatgg tcggggtagg ccaaaacctt
    18181 tataatatta aatttttttt ctaagaattc tatataagaa gtatttgtgg caacagcagt
    18241 caccacaaca gctgcaaatt tgtctttttc aaagctgtca attgtgaaag caggtttcag
    18301 agcagggaaa acaggtttag gctctttgta aaccattttt gtaaacaata cagtttgata
    18361 agcatacaat tttaggcgct tttcaatgag ccgttgatcg ataggcttaa ttgtatccgg
    18421 gcatttagta acaataatat aatcagctcg atcacgatcg cctgcaaact cgcgaagccg
    18481 ccccaatggg atgatttggt cttcataaat aggcctgtta taatctacta acagtatatt
    18541 aatgccaggt ttaacgtagc ggtgttggta tgcatcgtca agaataacaa ctttaagcag
    18601 agggttaagc tgttgtagtt tttcaatacc ccttacacga atagcatcaa cagcaacagt
    18661 aatattttca aacttacgtt tgatttgaca aggttcgtct ccggcctcaa caggggttga
    18721 ctcaggattg acaagacgaa agcctatagt ttttcttttg tagcccctgc tcaatgtggc
    18781 aacctcaaat tcagcgccga ggagctcgac aagatactcg acatgggggg ttttcccagt
    18841 acctccaaca gtaatgttgc ctaccgaaat aaccggaata tcaaattcgc gagaacgtaa
    18901 gatattgaaa tcgaaaagca aattgcggaa ccatacaatt actccatata ttatagcaat
    18961 tgggaaggca ataaactttg caaaaagatt taatttttga accatatata actaaggtaa
    19021 ataataaaga gttttaagtc cgaaaggcaa aaataaagaa atgaaggtaa taaaaagaat
    19081 cgtattgcac ttttttgaag atttgcctta aagttagttt aatgaaactt taaagattaa
    19141 ttacactcaa attaaatatt agaaggtaat tctttgtcga actattttaa ttagttgaag
    19201 taagttacag gtaaaggact taaatttttt ttctgctaaa gatgaagatt taatagcaaa
    19261 agaatgtcaa gaaattttaa ctttgtttga atagagataa acaaaatatt tagatgttat
    19321 gagctttgga gaatatttat taaatgtatt gccttcattt gtagttgggg ttccggtagc
    19381 atatttttat attacgaaga ctgtttaagg agtcagtatt taaaaggatc gggataattt
    19441 gggtcataaa cctgttgtta atagttgcta acacaacggc tgcgtgtgag tttaaaagtc
    19501 tttacccaca atatatatca gttccagtgg gaataatact ttccataatt ttatttgtaa
    19561 gggcagcaaa aattttgaag ctgttgcata aagctacaga agaccttgcg tctttatcga
    19621 gggggaactt aaatatagaa cttgatcctg atgccctcaa ccttaatgat gaagtaggaa
    19681 aaataaaccg ttcgatggaa ctgatgcagg aaaacttccg aaacgtaata tattcagtaa
    19741 agtcgagcag cgaaacactt gtaaatgagg caaaaaactt aaataaagca tcggctatac
    19801 tgactgaggc tgccaatgat caggcagctt ctattgaaga aatatcggcc tcaatggaag
    19861 aaatatcggc caatatccaa cactcggcca ataatgcaaa agagacagaa gtgcgttcga
    19921 aaaaggccag tgatacagta gaaaaagttg gtataacttc tcaaaagagc cagaaggctg
    19981 ttcaaaacat atcgcagaaa ataagtgtca ttaacgacat tgctttccag accaacatac
    20041 ttgctttgaa tgccgctgtt gaagcagccc gtgcaggaga acatggtaaa ggctttgctg
    20101 ttgtggctgc tgaggtgcgt aagcttgctg aaagaagcaa ggccgcggct gatgagattg
    20161 tacaaattgt gcaaaccact gttaatgaaa ccggggcagc caatgctctc gttgaagaat
    20221 tactccccga aataaaagat acgtccagac ttgtgcagga aattacggca agcagtattg
    20281 agcaatcaag cggagctgaa cagataaata attctattca gcaattgaac aaccgcaccc
    20341 aacaaaacat tgctaatgtt gatacaatga acaacagcgc ccacaggcta aatgaaagtg
    20401 ctgaacaatt ggagaaggca attaagtttt ttaagattta gattaaaaaa gctgttcgtt
    20461 tttatttgct atatgaagct tgatgataaa aaaatagaaa caataaaggg atatttcaaa
    20521 gccaggccgg ttttgaaagc ataccttttt ggttcttatg ttcgagggga tgcggatgaa
    20581 aaaagtgaca ttgatatttt agttgacctt gattattcgc aaaaaatcgg gttgcagttt
    20641 attcaaatga aaattgattt agagcaactt ttaagtgcaa aagttgattt agtttcggag
    20701 aatggattat ctaagtacat caaacccatt gtagataaag aaaaacttct aatatatgcg
    20761 aagtaaacta ggcgacaaag tcaggataca gcatatttat gatgccatta ttgaattgag
    20821 agttacctga ttggagctga ttttgataag tttatgaatg attcgatgat gaggtttgct
    20881 tgtataaagc aaatggaaat aataggcgaa gcaagcaacc atgtgtcaga ggaaataaaa
    20941 caacaatttt cagaaataga gtgggatcaa attgttggga tgagaaatgt gtttgttcac
    21001 gaatattttg gcgttgattc cactttaata aagagtgacc tgcccgaatt gaaagagaaa
    21061 gtaaaagaaa ttctaaactc aatagaataa gctgtaaaag cacttttaca atcatttctt
    21121 ctattgcgcg ccaaatatgg cagtatcatt ttccaacatt tttctggcgc actgttcact
    21181 atatttgaaa catttctaca aatatttatc cccgatgggg atagaattta cgctgataaa
    21241 attttagaaa gtctttaaaa taaaaaagcc ttccaacaat aaatgttgaa aggctttaaa
    21301 attatgtgaa atacgcttat ttcttcaaaa atttagcttt tatagtacct tttgaagtat
    21361 aagcatttac gaaatacagt cctgatttaa gatttgaaac gtttacactt tctgaatcaa
    21421 caatattaac agatttcagc acaatccgcc cgttgatatc ataaatatca atacttttaa
    21481 ttggcgaacc agatgaaata ttaatataat ctgaggctgg atttggatag atgctgattt
    21541 tagtagcatc aacagaattg atagcagttc gtatcccaat agtagcatac tgtggcaatc
    21601 cttcgaagat aaaaccagtt gccgcaacag gagtaacttc aaaaatcaaa taatagccaa
    21661 aatcgtctgt agtaacattg taattaagag aagtagcccc atcaattgcc accatattta
    21721 acccggtaga agctgttgac cgataccatt gatattttga agcaccttcc acatccttat
    21781 ttgcatcaaa ataagtgtaa aatgctacca agtcttggtc agtgctttca gttgctgatt
    21841 ttttgaccat aatatctccg gcaatattga catccatagc cttaggggca tcaataatgt
    21901 tagggttctt gagcctgatg ttggtcaata tcaggtattc gccggcttgc agattaaatg
    21961 ttgtttcagc cgaagtgaca ttaatactgt cgccggtcat gtagttgtac cagtatccgg
    22021 tacttgggaa cgtcagaacg ccagcttgtg catacaaatc aaagttgcca attatcctga
    22081 ctttcaccac agtatcgtca atagcaatag ttttgacttt agcagccaaa ttgagcgtgt
    22141 aattggacga attaaaagcg ttgagtttgg tcttcaatgc aatcaaggcg gcataagctt
    22201 cgtagagcct ttgacgattg ggattataat agtaatccca ttttataggc ttttcacctg
    22261 tacgtccatt gtattcaatt ttgtaatcat aattaagctc tccaaactgc cataccattt
    22321 taggtccggg aatggtaaag aaaaatgcag cacctaactc agcacgctgg caggctacgg
    22381 ggagtttgcg agttgcatac aatggattgc ttgtgtttcc ccaggttggg ttactatagc
    22441 ctattctttc ttcgtcgtgg ctttccatgt aggcaacaag ggcaggcttt gtcatgctac
    22501 gactgagata cgaaacgccg gtaaagttag ctttgccact ttcagtccag cccatagctg
    22561 catcgcggta ttgctggttc atgtttgccc atagcataaa gccatagtca gcaagcactt
    22621 gttcttcgtc attattggca aaatgttcca agataatata ggcatcttgc ttatatttac
    22681 gaatggtatc agaaatattc ttaaggattg caatacgcga gtcatcctta gtcccccaag
    22741 cagaggtatt tccaagggta ttcttttgag taaagccttt agaaaggtcg aaacgatacc
    22801 cgtcaatatt atactcagta agccaatagc gcaatacatc agcaacatac tgtttagtat
    22861 aagcactttc gtggttaaaa tcgtaaccca cgttgaaatc atgcttagca acaggattaa
    22921 accaggggct gtttgctgca ggccttgaat tgtttgcatc ccaatacatt tgcaccatag
    22981 ggttctggcc aaaagcatgg tttagcacaa catccaaaat aacagctatc ccatttgcat
    23041 ggcaggtatc tatgagcttt ttcaacgctt cgggggtacc gtaatatttg tccaaagcca
    23101 tgtggtatga aggattgtag ccccagctgc tattgccttc aaactccatc acaggcatca
    23161 gctcaatggc attaataccc aacgttttca ggtaatttag cgaatcaatc accgcattat
    23221 atgagtgttg ggcagcaaaa tcgcggacca acaattcgta tatgaccagc ttttggcttt
    23281 caggcatagg gttggaattg tacttccaat tgtattcggc aggtttggtt tgaagtacag
    23341 aaacaatgcc tgttgttttg cccaaaggat aagcctttaa gttaggataa acagcaaaag
    23401 ttataccttt atcgttatcc gggtcaagaa ccttgtgaca ataagggtcg gcaacgacca
    23461 agctgtcaac aacaaactgg taggcatatt ccttcccggc ttccaggtta tcaatctgaa
    23521 cccagaattt attcccatca gaggttttga acatttgata agttgtgtcc ggaatccagt
    23581 tgttgaaatc cccgataaca gtgactttat ctttcccggg agctgtgagt gtaagtaaaa
    23641 cagaagattc gctgataaga tttacaccat taacaatacc ggcaggcaaa tccttaactt
    23701 ttgtactctc agcacaatag aagctaaggg tatctgacaa tgtttcaacg cccttataag
    23761 ccctgacgac aattttcgag acacctgttt ttgaaatcgt tactgtatag gtaaatcttt
    23821 ctgttttatt tgttaaaaca ggcgttccat ctttaaacac taccgtagaa tcagcttcag
    23881 aagtaataac tttcacctca aatgaatcgt tgatttttcc gacattaatt ttgctcaaag
    23941 ggttaagcac aaccattttc aggccggcaa cagaaaaaac atcgtaaaaa atatctgttt
    24001 ctccattacc tttgccagtt ttagagccat cagcattcct aaacacaaaa gccagctttg
    24061 taaccgtctc ggactgggct acaccataat actcactaat ggaaggcttt attttcaact
    24121 gataggtatt gtcagcaatt tttgtaagct tatacttcgc agtattagta ggccatgtag
    24181 gggcatgttt ccaatcgcct ggcgaagagc ttgacgaaat aagcactccc gtatgggcat
    24241 acacatcacc agtataatcc atcaggcctt ttgagcccat atcggcatga aaggtaatgg
    24301 taaccgaatc ggtaaccttt ggaaatgcgg gactgcactc aattatttgg gcattggccg
    24361 aaaaatggct tatccacaat acaaagagta ccaataatgt agtttttgtc ttcatgtgtt
    24421 tagattttta tagaaataaa gttacaaagt ttatagtcag aatattaaga aagccgacat
    24481 tcgtttatac aaatgccggc aatttttcga aacctactta atatatacaa aaactaacct
    24541 ttacttttta acaatccttt gagtaaaagc ctttccgctg ctttccgaaa ttttaacaat
    24601 gtatgtcccg gcatttaagc cactaacatt tacagatttg atgttaccat aataattaat
    24661 caattgcttt ccggtaatat caaaaatggc gatgctattc acttcattaa cagccttgat
    24721 attcagaaca tcgtaacaag gattaggata acaggccata tttgccgaaa cagtgctgat
    24781 agaggtttgt gcaggctttt cattgttgaa ataaaaaaca ggaataacat tggttgactt
    24841 aaccccggta gtgtcaaata cattatatgt acgtgcagca cctccggcag ggaattcgtg
    24901 ttttgcatcg tcccagctac catcaatacg gcatttaaac tgaatctctc ctatagggca
    24961 caatatggtt gccgaatata cgtcttcagt tccaaacaac tcatcaggag aagctcccca
    25021 atcgttgaac gtaccagcaa catctaaaaa gttggtttca gggtcgaact tacctttttc
    25081 tttagcaacg ctcatgttta ccgaaatttg tacaggaacg tagcccgggc ggtaattgtt
    25141 aaaaatcttt ttaaccccga tactatcttt tcggacagtg attttcctgt ttgggccacc
    25201 cgaaggaaac tcatgtttgt cgttgtccca gctgccgtta atcctaaact tatactctac
    25261 aacagtgcca gccgtaaagg tatcaatagt aatagcgtaa aggctgtcgt tacctggcat
    25321 aggggcaaga tgcgaattag atccatccca gccgttcagg gtcccggcaa tatcaacaaa
    25381 gtcatttgtt ttgtcaaact taccagcttt aatcatatag gtcatgtctg caatgaaagt
    25441 aattttagca ttttgagcaa ttgttaagcc ttttatcatt aaagctgcaa tcattagaag
    25501 aagtacatgt tttttcataa ttaagttaat ttagtttgta aaaaagaatc tttgacacga
    25561 agttattgga caaaacaagg gaaaccaaca aagcatttgg aagctaatgt aaaaaatggt
    25621 acttttcagt cataattttg agatgctata aggcatttca aacgtttgca taaaaagggc
    25681 ataaaatatt tgttttttaa ggttttatgc tttgacaaaa acaaaaatga agctactttc
    25741 accacatgag aacagcacaa acaacaataa aagacatagc ctcgcgctta gggctttctc
    25801 cctcaacagt ttcgagggca ctgaaagacc atcccgacat cggcgcagac actaaaaaag
    25861 ccgtgaaaga cctggctgtt gagcttaatt ataagcccaa tgccatggcc ataagtttgc
    25921 gcaatagccg aagcaacatt atcggcgtca ttatccccga aatagtacac catttctttt
    25981 cttctgtcat atcgggcatt gaagacgtag cttatgatgc tggttaccag gtaatggttt
    26041 gtcaaagcaa tgagctttac caacgtgagg taaaaaacac ccaagccctt cttttgtcaa
    26101 gaattgaagg cctgctcgtt tctgtatcta aagaaaccag ggacttttcg catttgcggg
    26161 gtgtaaaaga gctcaatatc ccagtagtgt tttttgacag ggtatgcgac gaaatggaag
    26221 ccgaccgggt cattgtggac gactttgaag gagcttataa aatcgtatca catttgatag
    26281 aagtgggttg caaacgcata gcccacttag gtacagtgca agatttactt attgggcgca
    26341 acaggctaaa cggctacctg aaagcactca aagaccacaa tttgccgata gatgacaagc
    26401 ttatccttcg ttgcgatacc atgcaatctg cacaaatagc caccaagcgt cttgtatatg
    26461 agctaaatcc gcccgatggg atatttgccg ttaatgacct caccgccatt ggggcattac
    26521 agacaataaa agaatgtaag atgcgtgtcc cggatgatat atgcgtagca ggctttggaa
    26581 atgggctgta ttcaatgttt accgatcctc caattaccac tgttgaacaa aacggttata
    26641 aaatgggaca agtagccatg acacttatgc tcgaccgcct tatcagacat gacgactacc
    26701 ctcctatcac cgaggtgctt gaaacaagcc taatacaaag aaagtcaaca cagaaaccta
    26761 ttaaaccgca gtatatttaa cggagcaagg aaaaaggaaa aaaggttgaa gattttggtt
    26821 attatgaatg ccaataacta tcaaccaaca tctatcaaca ataaaatgaa gaaatctaaa
    26881 aaatacctgc cctacatatt actggcattt ggattattgt gtattgggtg gtcggctgtt
    26941 tttgtgaagt tggctgatgt cagtggctta gtttcgggat tttaccgcat gtttatagga
    27001 gccatttcaa taatgccctt atggctgtta cgccacaaat catggaaaat agatgcaggc
    27061 tcatttcgca ttgccgtgct gtgtggagtg ctttttgcct gcgacatagc cttgtggaat
    27121 acctcaataa tgctgtcgaa agccgctata gccactctgt tagctaatat ggccccggtt
    27181 tgggtagggc ttggcagcta ttttatcctg aaagaaaaag cagggcgcaa tttctggatt
    27241 ggctgcatca tttctatcat tggagtatca ctaattacag gcattacaag tatctcagcg
    27301 tccatgtttt catggggggc aatattggcc ataatggcaa gtttttttta tgctgcatac
    27361 cttttaatta caccacgtgt gcgaggaaaa gtagatacaa tttcttttac agcagtatct
    27421 atgatagcaa gcagtgttgt gttgctcata ttttgtatgg tttcagagag caaacttaca
    27481 gggtacagta ccaaatcatg gatggcgttg gcaggcttag gctttgtatc gcagttcctg
    27541 ggctggatag ctataaactg ggcgcttgca tatattaatt ctacaacagc cagcgttacg
    27601 cttcttagtc aaagcattgt aactgcaatt attgcaatac ccgtgttagg cgaaacattg
    27661 tcatttatag aaattattgg tgcaatagtt gtactaaccg gcatattttt ggtaataaag
    27721 aaacaaaaag ccagcaaaac agaaatattg ccggagtatg aatgatacca cccaagtaaa
    27781 aatgccacta aatcccctaa aggggacttg gactaatgcc tctttaagta taggaagagt
    27841 ttttacaatt tttattaaaa aaagttcttt attttcagac attttgaagg taaaaattta
    27901 tctattgctt atcattgctg ttactatatg ctcatgccga cctacattta aagtcagaaa
    27961 gctgagttat gatgacagta ctggttttta taagtatgat aaaagcgttc ctgttatatt
    28021 acctgataaa aggcttgtaa ataaagataa tatccaggca ttcatacagt ttatagccag
    28081 ttataggtat gccgacaaca acaatcttct ttatcagcct gactgggtgg tttccaaaaa
    28141 agataaagag ttgcttgatt catataataa ggtcattcaa aatatactaa ataaagaata
    28201 ccaaatttca ttgtcagaag ccaagacctt taaatcaaag ctgaatgggg ctggaggttt
    28261 ttctgatatt gacttttgga ttgcttatgc tcacctcaat ttgtcgaaca atgattcggc
    28321 agcttattat tttactcagt ttttgcttta ttcgggatac aaatacccga taaagttcag
    28381 aggccacttt aatagtgagc atgccacccg gcttttggcc gaacaaaaaa aatatgcagc
    28441 agacttttta gaaggaagag aagatttacc tcctgtaaag ccagagatag ttaagcatag
    28501 gtattattac caaagccata ccccgggttt tataatcaat cacgaagatg caggcgtgaa
    28561 aaactttaac ttttttgttc ttcccatact gtatgacaac aattttcagg gaggacaagg
    28621 gggattttca tacaataaat tcaaaacact taatttttct gtatattcag aaatagaaaa
    28681 agaacaacaa tctttaaaaa taaggatccc gtggcaaaca tatatatcag ccacgcacag
    28741 attaggaatt aaaataactc cacaatgccg tttttattcg agtgttatca atgatgaaaa
    28801 ttataaaggc atattgacag gaagctctgt ttctatagga tacaggataa cacaccgtgt
    28861 ttatgcaggt atagcttata caacactata ctctacccat gatgcattta tcaacaaaag
    28921 gttttatcgt gcccaacaac tgcatggcag ctatgatgcc gggattcatt tacaacttat
    28981 caaatctttt tctttaaaac ttggttattc agacggttat ggcatgtttg gcttgctcat
    29041 caacaatgtt ttctcgggtt ataacgaaaa aacgaagtca agcatgttcc aatttaatga
    29101 tttctaaaca ttggcttaat ctttgttgtt tacattgcga caaaagaaaa tactatgtgg
    29161 aagaaaattg gtgctattac tcttgtttta tttttttcat atatcgtcaa cgcacaaaat
    29221 gccgatgata tatgtggata ctggcgtaca ctcaaaggaa atacacaatt tcatataaca
    29281 aaaaacgaaa atgggctcta tagcattact attgtatggt tgcgtataaa aaaagaccgc
    29341 acagatgcaa acaatcctaa tcctaatctg cgcaacagga agtttcttca attagtaatt
    29401 tcagataata ttgtattttt gaaggataaa aataagtggg taggt
//
LOCUS       DAOSPF010000043        29382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1593_length_29382_cov_6.961060, whole genome shotgun sequence.
ACCESSION   DAOSPF010000043 DAOSPF010000000
VERSION     DAOSPF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_29382_cov_6.961060"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..876
                     /locus_tag="PK252_09415"
     CDS             97..876
                     /locus_tag="PK252_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOT14964.1"
                     /translation="MLPDKRQIEICISPALYHKFHKDEAIVVVVDILRATTAICAAIE
                     NGAKEIRPVSTLEEAREFKNKGFWVAAERDGLVQDFADFGNSPFNFTPERVKDKTIVY
                     STTNGTHSIRMAQKSAMVVIGAFINFSALKDLLIAENKDVVMLCAGWKDKFCIEDTLF
                     CGAMAEALMASNLYGTSCDSTYASIDLWQVAKGNVLQYIEKAAQRHRLKKNKLDDVLE
                     YSFSFDKTKSVPVLMGDAILAYKKDKWEKFLQIKKGEAINY"
     gene            922..1995
                     /gene="serC"
                     /locus_tag="PK252_09420"
     CDS             922..1995
                     /gene="serC"
                     /locus_tag="PK252_09420"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOT14965.1"
                     /translation="MRKHNFNAGPSVLPEFTLEQTAKAVLDFNGSGMSILEISHRSKD
                     FEAVLAETKALFKELLNIPDGYSILFLGGGASLQFCMVPFNLLNKKAAYLNTGVWASK
                     AMKEAKLFGEVVEVASSKADNYTYIPKNFTVPEDVDYFHVTTNNTIYGTELKTDLSVN
                     VPLVADASSDILSRPMDVSKYGLIYGGAQKNLGPAGVTFVIVKNDILGKVTRPIPTML
                     DYKTHIENDSLFNTPPAFGIFAILQTLKWIKSIGGINAIYKMNLEKAALLYEEIDRNP
                     LFRCPVSVAEDRSIMNVCFVMNEQYKELENEFLELTKTKGIVGIKGHRSVGGFRASTY
                     NALPKASVEVLVAAMQEFAKQKA"
     gene            2018..2941
                     /locus_tag="PK252_09425"
     CDS             2018..2941
                     /locus_tag="PK252_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT14966.1"
                     /translation="MKKVLVATEKPFAKAAVESIKQIVEGAGYEFKTFEKYTTQEELI
                     QAVADANALIIRSDIVDSKVIEAAKNLEIVVRAGAGYDNIDLAACSKRGIVAMNTPGQ
                     NANAVAELVFGMVIYLIRNQFDGSTGVELRNKKLGIHGYGYIGKIVALIAKGFGMKVY
                     AYDPFVDRVLIENDGVKYESKLEDLYTKCQYISLHVPANEKTKKSINYDLLKLMPEGA
                     CLINTARKEIIDEEGMLKLFEERKDIKYASDIQPDCAAVFAEKYKGRYYFTPKKTGAQ
                     THEANNNAGLAAANQIVNFFKNGDKTFQVNK"
     gene            3115..3798
                     /gene="tmk"
                     /locus_tag="PK252_09430"
     CDS             3115..3798
                     /gene="tmk"
                     /locus_tag="PK252_09430"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT14967.1"
                     /translation="MKFVVIEGLDGSGKSTQVGLLKEYLKKNNISFQYIHFPRVETGI
                     YGDMVARFLRGEFGNNDQVNPYLVALLYAGDRMDAAGQINQWLANDELVLLDRYVISN
                     IAYQCAKVVDKAEKEKLKNWILQLEYEHNKIPVPTINIFLDVPFDFTRERLTQGRTGD
                     DRDYLNGATDIHEADLNFQEKVREVYLEFGKDGKALKIISCNENGKMLPPDIIFEKIL
                     NILKNENVL"
     gene            3918..5168
                     /locus_tag="PK252_09435"
     CDS             3918..5168
                     /locus_tag="PK252_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOT14968.1"
                     /translation="MERFVKYLFHFCRISIGLLFIFSSFTKGLDPWGFAYKLTDYFQV
                     ININNLHYSTLALSFIASGLEFLLGVMLLFNVLMKYTAWAAAAFMAFFTVLTFLLAWF
                     NPVSDCGCFGDAIKLTNWETFFKNVVFSVLVAVIFWKRNKYKVSKNTYLTYGLITSGI
                     GVFVLFSVASYRHLPIIDFMPYSIGSNIKEKMTVPPGAPVDKYETMLTYQNKKTGEKK
                     DFDLKNYPWQDSLTWKWVETKSVLVEKGYTPPIHDFVISDHEGNDITQQILNDSGYVF
                     LFISYNLKLANAAALKNADSLYLYYSGKPDTRFYAVTASTVGVQSQIKDSLGLNMAFN
                     TGDETMLKTVIRSNPGLVLLKNGTVLGKWHYNDIICQDFFQHPDLLAYSVHKLNRGNE
                     VMFTALIFAFLSILILFLYYYNKK"
     gene            5182..6273
                     /locus_tag="PK252_09440"
     CDS             5182..6273
                     /locus_tag="PK252_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT14969.1"
                     /translation="MKTLILYILKKALYSVSILWGVMTLIFILFNVIPGDPARMALGQ
                     RSDSASLAAVRADLGLDKPVTMQYVKYLNDLSPVSVFNCKDQSSYFFYDTTVYKHSAK
                     LFNIGESKGLVLKAPYMRRSYQNKRFVSDMISEALVPTFVLAFISIVLATIIGIGLGI
                     LSALKKDTWVDKVSIVVSALGMSLPSYFAAILFAWLFAYLLGNVTGLDLTGSLYVIDD
                     MGEGRHLALKNIILPALTLSIRPLSVFTQLTRNSMLDTLSQDYIRTARAKGLSQYSVV
                     MHHALKNALNPVVSSISGWFASMMAGVIFIEYIFAWKGLGYVMVNALQNFDVPVVMGT
                     VVVISVIFIVVNIFTDIVYSILDPRISLK"
     gene            6369..6770
                     /locus_tag="PK252_09445"
     CDS             6369..6770
                     /locus_tag="PK252_09445"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT14970.1"
                     /translation="MKKVLLTAIFGFFMYDIFAWGIDIYKVKNAPVIDGNAIDATWAL
                     QPLNDIEQALGSTDGYSGSFKMCYDDKNIYLLLYITDPTPNQVGSSTWQSDCVELFFA
                     MDVKNSDTYRPGDWQIRKLASKTQAAGGGRW"
     gene            6835..10329
                     /locus_tag="PK252_09450"
     CDS             6835..10329
                     /locus_tag="PK252_09450"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOT14971.1"
                     /translation="MVQEWQIPINTLVETANFNGSSFRFDIQLSDNDGTGNERTGHIY
                     WNSTADDQWTRIQNQGMIYLKAEQPSLEVPIEMGSTWRFFKGKTAPSVEWINPSFDDL
                     SWTASSAPFRFGDGTGGIELTDMLNNYTSIFLRKTFTVNMPDSLISECNATMVIDDGY
                     KIWLNGKEITSYNIPLSVKYNSLASSAVEMQTISNSFKIEDVGLKNGQNTICVQVLNC
                     SLTSSDLYFDMMLTLNKKLPKAPEVKFSKPGGYYNAKFNVTLSGGQDGDTIKYTLDSS
                     DPQTSSTAILAVSPVTIVIDPDDNTRIVKTPGIVVRAVVQKTGYNSGNPESRTYLFLE
                     KVKTQVYPGGTWPSSAVNNQIMDYDVNQSVVSQYSSDFSKAFTTIPTVCLVTDNIGLF
                     DASQGMYVNALNHGESWEKAASIELINTDNTEAFASGIGLRIRGGWSRHPDNPKHAFR
                     VFFKDKYGKKKLKYPIFGEDAAQTFDKFDLRCAQNYSWSYYHDNMLTYAQDETCRDIQ
                     GLMGHTYTRSRYCQLFLNGMYWGLYEFMERPEANFAESYKGGDKDDYDVIKVATDNSS
                     NLEATDGNMDAYQRLYSFTQSGFTADAAYYKLQGLDKTGNIDTTLEKLVDIDNLIDYM
                     LNIFYSGNFDAPLSEFGGNNQPNNFYAIKNRNSKREGFFFIVHDAEHTFNYISGSQEN
                     NNSGVQENRVSLENDGMSSPSSYLKFTPQWLHHRLKSNAKYRLRFADRAVKYLYNDGL
                     LTASQVEKVFKARTNQINMAIIGESARWGDSKSSYDNSHTKDNAWIPAVNNTVSKFIK
                     LRTPIVIQQLKDAGLLPKTDIPVVKQDGKAVSVNKVEISKPIKVTFENPNVNGKIVVS
                     TDGNDPMNIDGTQYSSATTFANGEEMDISSATIVKARIKTDTEWSPLREVIFTNKANR
                     ENIRITEIQYNPAATASFGSKSMEFIEFKNIGTTGIDLSGCRIDSAVKYTFPEGSIIA
                     PGKFVVIAADITAFESTYLKAPTGRFSGNLANEGERVILYDESNTALIDFTYKPDWVL
                     RANETGFSLVAKSKTPDTKPEAGTTYWKGSVLIGGSPFADDEGYIPPSEIETSKITCS
                     IYPNPASSVIKIEAMGSNFRNLKMVDLQGRTIYNENLRSDRISMSLNLQQLQVSQGIY
                     FIVLKNDKEMKVQKVVVK"
     gene            complement(10438..11388)
                     /locus_tag="PK252_09455"
     CDS             complement(10438..11388)
                     /locus_tag="PK252_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HOT14972.1"
                     /translation="MYRKEIKVIDCTIRDGGLMNNWQFSDEFVKAVYHALVEAGVDYM
                     EIGYKSSEKSYSRTENGPWKFCDEKDLRRIVGDNKTNLKLSCMADIGRIEPTDIAPKK
                     DSVIDMIRVACYVHQMDKAIALAEHCMDKGYETTINLMAVSKVNERDLDEALNDVAKS
                     RVPTLYVVDSFGSMYCEQIELLARKYFKALPGKTIGIHAHNNMQLAFSNTITAIIEGC
                     NMLDSTLLGFGRGAGNCHTETLIAFLKNPKFKLRPIIKVIQEQIIPLQQKIDWGYHIP
                     YLITGMLNEHPKSAMEWMESPEKYDFVKFYNQMTESYALE"
     gene            complement(11411..11752)
                     /locus_tag="PK252_09460"
     CDS             complement(11411..11752)
                     /locus_tag="PK252_09460"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT14973.1"
                     /translation="MTPKIKPELIKAMTKKAYQCLSLEEVRKEIDSIDQQIIQLFADR
                     FEYVKAVVKFRNPAQPEVADRVRFENVIKQRGQWASNLGLDANSIEKVYRELIEYYIR
                     EQAEYAKNLIK"
     gene            12049..13566
                     /locus_tag="PK252_09465"
     CDS             12049..13566
                     /locus_tag="PK252_09465"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT14974.1"
                     /translation="MADNKVFIFDTTLRDGEQVPGCQLNTIEKIEVAKALEALGVDII
                     EAGFPISSPGDFNSVVEISKAVSEPTICGLTRAVKKDIEVAAEALKFAKRARIHTGIG
                     TSFYHINYKLKSNPEEIIERAKEAVKYARKFVDDVEFYAEDAGRTENEYLARVIEAVI
                     AAGATVVNIPDTTGYCIPEEYGRKIRFLKENVKNIDKAIISTHCHNDLGMATANTIAG
                     VMNGARQVEVTINGIGERAGNTSLEEVAMILKCRNDIKLNTGINTKKIISTSRLVSTL
                     MRMPVQANKAIVGRNAFAHSSGIHQDGVLKHRENYEIIDPREVGLSESAIKLTARSGR
                     AALKHRFEKLGYKVDGDKLDKIYEQFLAMADKKKDINDDDLVLLLGEDRSKDKIIQME
                     YLNVLCGKSAIPMATVKLKYNGEEHVATSSGVGPVDAAFSAVRQIVKTKAKLEEYLVQ
                     AITRGTDDIGKVHVQVEYKGVNYYGFGANSDVITASVEAYIDAISNCIKANPGKN"
     gene            13587..14963
                     /gene="leuC"
                     /locus_tag="PK252_09470"
     CDS             13587..14963
                     /gene="leuC"
                     /locus_tag="PK252_09470"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT14975.1"
                     /translation="MSAKTLFDKVWERHVVHQIEGGPAVLYIDQHLIHEVTSPVAFKG
                     LDRRGIKVRRPDRTLATPDHNIPTMGQELPIKEELSRMQVQQLSENCKKHNITLFGLG
                     HKYNGVVHIIGPELGITKPGMTIVCGDSHTATHGAFGSIAFGIGTSEIEMVLASQCLF
                     QAKPKSMRINVNGKLGKGVTSKDIILYIIAKLTAGGGTGYFVEFAGDTIRSLSMEARM
                     TICNMAIEMGARGGIIAPDETTFEYIKGREFAPKGEEWDKALAYWKTLPTDEGAKFDI
                     EYSFDAADIEPMITYGTNPGMGLKISQNVPSDEPNKQALEYMGLKAGDSMIGKPIDYV
                     FIGSCTNGRIEDFRAFASVVKGKKKADNVTVWIVPGSKQVERQIEAEGLKAIFEAAGM
                     QLRQPGCSACLAMNEDKVPAGKYSVATTNRNFEGRQGPGSRTFLASPLTAAACAITGK
                     ITDVRTII"
     gene            15044..15628
                     /gene="leuD"
                     /locus_tag="PK252_09475"
     CDS             15044..15628
                     /gene="leuD"
                     /locus_tag="PK252_09475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT14976.1"
                     /translation="MEKFQTITSSCVPLNIENVDTDQIIPARFLKATSREGFGDNLFR
                     DWRYDNENKPKPNFVLNNPAYSGKILVAAKNFGCGSSREHAVWAIYDYGFRAVVSSFF
                     ADIFKNNSLNCGLLPVQVSDAFLAKIFKQVDADAKTLLKIDLENQKITIVATGESEPF
                     EVNSYKKECLLKGYDDVDYLLSLKDKIEAYEKAH"
     gene            15792..17309
                     /locus_tag="PK252_09480"
     CDS             15792..17309
                     /locus_tag="PK252_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOT14977.1"
                     /translation="MAKVIEIMDTSLRDGEQTTGVSYSDSEKLNIAKVLLDEVRVDRL
                     EVASARVSEGEYKGVQTITSWARKAGHINKIEVLGFVDGGKSLEWIQKAGGKVINLLT
                     KGSLKHCKEQLRKTPEEHFADIIQTILLAEKMGIMVNIYLEDWSNGMRHSKEYVFSMI
                     DALKNQPVKRYMLPDTLGILNPDETFQFCHEVVSRYPGLHFDFHPHNDYDLAAGNVFM
                     AVKAGIQGIHTTVNGLGERAGNVPLASVVAVLNDHFNAKLPVVESSLNKVSRLVESFS
                     GIRIPVNKPIVGEYAYTQVAGIHADGDKKNKLYYNDLHPERFGRQRKYALGKLSGKAN
                     IVQNLEELGIELTEEEMKMVTERIIELGDRKEYVTREDLPYIIADVLQSNIVKEKVFI
                     KNFSLSVAKGLRSVATLKLSIDCHEYEESASGDGQYDAFMNALIKIYHGLNKHIPELL
                     DYVVSIPPGGRTEALVEAMITWRIDGKEVKTRGLDSDQNVAAIKATMRMLNMIEK"
     gene            17310..18245
                     /locus_tag="PK252_09485"
     CDS             17310..18245
                     /locus_tag="PK252_09485"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOT14978.1"
                     /translation="MHSIKNIAVIGIGGVGGYVGGKLAQLCASDEFQVNVSFIARGAH
                     GQKIANEGLTLHVAKETFKVLPSEVIENISHLKSPDLVVLATKSYDLGPVAGQLDKVI
                     RKDTLIMPLLNGIDIHQRIRKVTSKGIILPSCIYIASFINAPGEVTVLGAKVLINTGH
                     TNPFEKVNKEAVISLFEKAGLKLKWNENPFLAIWEKYAFVASFAIVSGCTRQSFIEMV
                     NEPSSNQAVRDVMSEIISLARAKDIILPADFVEKSMETAKKLSPDAKTSFLRDIESGK
                     GLNEADIFGETVIRLGKELGISTPVTSKMVQGTKF"
     gene            18284..19342
                     /gene="leuB"
                     /locus_tag="PK252_09490"
     CDS             18284..19342
                     /gene="leuB"
                     /locus_tag="PK252_09490"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT14979.1"
                     /translation="MGKTFNVAVMSGDGIGPEVVAQAVKVLKAVEKKYSVSFNFRDVL
                     IGAIAIDQTGNPLPDETIRICKESDAVLLGAVGHPKFDNDPSAKVRPEQGLLKIRKEL
                     GLYANIRPVTTYSLLNEASPIKNHLLKDVDMVVYRELTGGIYFGEKGRKDEGKTAYDN
                     CIYTEAEVLRISHMAFKMAQSRKKKLTMVDKANVLESSRLWREVVKKLEAQYPDVTVD
                     YMFVDAAAMKLVTWPAYFDVILTENMFGDILTDEASVITGSIGLLPSASMGDKYALFE
                     PIHGSYPQAAGKNIANPYGTILSAAMLCEYLGLADAAKDIRNAVNKGLEQGVVTEDIN
                     KTKNNTTTEVGDFVAANL"
     gene            complement(19483..19938)
                     /locus_tag="PK252_09495"
     CDS             complement(19483..19938)
                     /locus_tag="PK252_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14980.1"
                     /translation="MNHSLLKYRDIIQWIHDRYNKTVDVDNISAALHDVSYDDVTKTF
                     TYKCIDADEGFHKLFQTDNTSLASLLYENIFPETKENKFNWGALIGHVGRFGSISSFY
                     AYSPNTHKWYSIMAISPEMNKTLLLFYDITEEKNLEIAKNIQRNSASSA"
     gene            20498..21838
                     /locus_tag="PK252_09500"
     CDS             20498..21838
                     /locus_tag="PK252_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14981.1"
                     /translation="MKLSTSLSCIAFAMFPFMTNIMGQTAATAETANPPAESQVSLGE
                     NVKMKIGGFARADFFYDTRKNVEILDGLLDMYPLNKSLDANGKDINDVSLLRMSAAAS
                     RLNAKFTGPDFLNAKTSSLVEFDFTGVNGIGLRLRHAWVKLNWEKTELLFGRFWHPMF
                     ILDAFPTVLALSTGAPFCVFNRAEQLRFTYNLGQISFMAAAAGQMDYGFSSEYNSATG
                     ATSYLHNQIMPDITVNAQFKNDNIIFGASANYKVNQPRLYTQDASTPANKYKTDEKIA
                     SLSAQAYAQYKTGLLKIKGGALYGENMMELLMMGGYAVKTIDTVTFKETYSTVNNLNY
                     WCNIVYGDKLQAILFVGYMQNLGTVDKQIVGFTKNSARGYDEKGNGIANMIRIAPGVA
                     YKVGRVQMHLELEHSITAYGKYTNAANVINENAEVKDTKNISNTRVQFATIFYF"
     gene            21868..23178
                     /locus_tag="PK252_09505"
     CDS             21868..23178
                     /locus_tag="PK252_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT14982.1"
                     /translation="MNQQYWEKELETLSQVDLQILQYERLKKTLRFASQSMYYKKLFA
                     EKKINPDGFKSIKDIEKIPFTTKEDLRSNFPYGFLAAEKREVIRLHSSSGTTGNPTVV
                     YHSKHDIDSWANLVARSLFAVGTRDTDVFQNISGYGLFTGGLGFQYGAERLGALTIPA
                     GAGNSKRQIKLMRDYGTTVIHTIPSYVMHLVDFFREMNLDPRTDTDLRHMVIGAEPHT
                     EETRQRIQAMMGIKAYNSYGLSEMNGPGVAFECEYQNGMHLWEDAFYAEIIDPVTLQH
                     VPEGEIGELVLTSLDRQAMPILRYRTRDLTRFLPGSCPCGRTHRRIDRMKGRSDDMFI
                     IRGCNVFPMQIEKVLMQIPEVGNNYMIQLETIDGVDEMIIEVEVLDGNFLDNYSKLEK
                     LSKIITNEVKNEVLVTPKVKLVQPNHFPPCEGKALRVKDNRKQY"
     gene            23289..23720
                     /locus_tag="PK252_09510"
     CDS             23289..23720
                     /locus_tag="PK252_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOT14983.1"
                     /translation="MSIKQLSIFLENKSGRLTEVLETLGQEKINIAAMSIADTSEYGI
                     LRLIVSDPEEALRLLKNKGFSVNMTDVIAIETPTYAGAFAEALKHFSDAQVGIEYMYA
                     FELDNNGIIVMRTDDRQKAFKIIESNKLRLVSGNDIFKEKK"
     gene            23742..25043
                     /locus_tag="PK252_09515"
     CDS             23742..25043
                     /locus_tag="PK252_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT14984.1"
                     /translation="MIWNKTFECMSREEMRECQSKRLIETVQRVYHNVPAYREKMQQA
                     HLVPEDIRSVDDLHKLPFTVKQDLRDNYPYGLFAVPMSEVVRVHASSGTTGKPTVVGY
                     TRKDLATWSEVIARTLTAAGAHKHDVIHIAYGYGLFTGGLGLHYGSEKIGATVIPISG
                     GNTQRQIQLMHDFGSTVLACTPSYALYLAEAIDESQYKRSDLKLRVGVFGAEPWTETM
                     RKEIEEKLHIKAIDIFGLSEVIGPGVSSECEHQCGLHVFEDHFIPEIIDPKTLEVLPE
                     GELGELVFTCITKEALPLIRYRTRDLTRLHYEKCKCGRTLVRMEKCTGRSDDMLIIRG
                     VNVFPSQIEEVLLKIDGLQPHYLITVDRVHNLDVMQLQVEVEQEYFTDRISELQGLSK
                     KLQHSLESALGIGVDVKLVEPKTIQRSEGKAKRVIDNRKLN"
     gene            25150..25329
                     /locus_tag="PK252_09520"
     CDS             25150..25329
                     /locus_tag="PK252_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14985.1"
                     /translation="MLGITDPWILAAYLLSFLSAVACIVYGLYNWNKGGNNEESQITE
                     EVKWEKEEKQIEENL"
     gene            25347..26963
                     /locus_tag="PK252_09525"
     CDS             25347..26963
                     /locus_tag="PK252_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOT14986.1"
                     /translation="MLFDIIVVLIYLFVITYLGYLGYKHTKSSADYLVGGRKIHPLIM
                     ALSYGSTFISTSAIVGFGGAAGTLGMGLLWLTFLNIFMGIFIAFIVFGKRTRQMGHNL
                     DAHTFPELLATRFKSKFIQGFAGGIIFLFMPIYAAAVLKGGSDYIQAHFGISYEVSLF
                     FFATIVAIYVWMGGIKGVMYTETFQGAIMFIGMIFLLVYSYASLGGITEAHTKLTNLF
                     GHAEVQDQAAKLVKGGFMGWTSMPAFGSPIWWTLVTTIVMGVGIGVLAQPQLVLRFMT
                     VKSNRELNRAVISGGIFIIMMTGVAFIVGALSNVFFFESTGKISVVAAGGNDKIIPVF
                     IKQYLPTWYGAIFLLTLIAAAMSTLSSQFHAMGSAAGRDVYQKAFNKKGNPLFISRTG
                     VLVGILISVVIAYFSGKLDVSMQIIATGTALFFGLCAASFLPAYVSALYIRKISTKAV
                     ISGMIAGFVVGVFWIFFIHEKEASSLQLCNLLFDKPSLVKETALAKLALVDPIIIALP
                     VSTLVTAIVGVTAKSKLDEAHLDNCFKGIK"
     gene            27086..28681
                     /locus_tag="PK252_09530"
     CDS             27086..28681
                     /locus_tag="PK252_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOT14987.1"
                     /translation="MSITQLLLTLLPIAVILVMLIVFRKAADISGIAGWLAVSLVAFF
                     FFNTSIEVLLRSTGAGLIRSFSVSLIVATSLLQMAFMEKTGALKRIIIFVKTISSESR
                     AIQIMLINIGFGTLMVAVGATPVSLLPPILVAMGYSTYVAIALPAIGYDSLCTYALLG
                     APIVVFVDVANNFLGPGNEISLHQAGMVFYMFLPVVSTLIGFCMLWIVGKWQAIKEGW
                     VPCLVTGLTIGVVAYFTNRYDNLVVLTGVMCGIAVIAMMVLFLKVKGMKVIDKSRLTP
                     EELEYEKSYPLWKALMPWLILIGLILMLNLPKDVFNYLYRTLKLPVTGLCADGKPLDT
                     RFLWQAYTWIFVSVVLAIPFLKPTKAQMKDTLKVWWKRAPRPVFAAAIFFAIGEIMNM
                     SGYSIVDKGFVVPSMIKVLATYSADFFHSAYGAVVTFIGLFGGFITGSEASTIAMFAK
                     YSLTTAKELALPVTGLIIITAGLAFGGGLASVISPAKLQNAAASIDKIGEENKVIRIA
                     FVFALILTTVTSLFTVLLLLLNC"
     gene            28763..29248
                     /locus_tag="PK252_09535"
     CDS             28763..29248
                     /locus_tag="PK252_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT14988.1"
                     /translation="MQIQYKILKRTFIPQIILSVIIVLIIRGFIYYTHKPIDTFYFVC
                     ICVMIISNVFIFYPNVKKIGLLQFDILGVTVNMNNSAIRYNFLDENIKSLNIKLKINK
                     NRLHTKYPNNSAVNALNKLEIFYNDQIIKYNFKADSVDDLKKLKEMLVKINEKAKILL
                     N"
BASE COUNT         8965 a         5138 c         6221 g         9058 t
ORIGIN      
        1 gtttttcggc gagtgttaaa aaaaaaacag aaaaacgtgc aaccagttga tgtgtttttc
       61 taattttgcc gttctaattt ttggagaaag gggaaaatgt tgccagataa aaggcaaata
      121 gagatttgta tttcaccagc tttatatcac aagtttcaca aagacgaagc aatagttgtg
      181 gtagtagata tcttgcgcgc aacaacagca atatgtgcag ctatagaaaa tggggcaaaa
      241 gagattcgcc cggtttctac ccttgaagaa gcacgagagt ttaagaataa agggttttgg
      301 gttgctgccg agcgagatgg cttggtgcag gattttgcag attttggaaa ttctccattc
      361 aattttactc cagaacgtgt aaaagacaaa acaattgtgt atagcaccac taatgggacc
      421 cattctatca ggatggcgca aaagtctgca atggttgtca ttggtgcttt tatcaatttt
      481 tcagcattaa aagacttatt gatagcagaa aataaagatg ttgtaatgct ttgtgcaggc
      541 tggaaagata agttttgtat tgaagatacg cttttctgtg gggctatggc cgaggccctt
      601 atggcaagca atttgtatgg gaccagttgt gattcgactt atgcatctat agatttgtgg
      661 caggtagcca aaggcaatgt gttgcaatat attgaaaaag ccgcacaacg gcatcgtttg
      721 aaaaagaaca aactggatga tgtcctagaa tatagtttca gttttgataa aacaaaatct
      781 gttcccgttt tgatgggaga tgctattttg gcttataaaa aggataaatg ggagaaattt
      841 ttgcaaataa aaaaaggaga agcaataaat tattaattta gcagtcaaat ttttaatcaa
      901 tataagaaaa ttttattcat tatgagaaaa cacaatttta acgccgggcc atctgttttg
      961 ccagagttta cactcgaaca aactgcgaaa gcagtattag actttaatgg ttccggaatg
     1021 tccattcttg agatttcgca tagaagtaaa gattttgagg cagtattagc tgaaacaaaa
     1081 gcactgttca aagaattatt aaatatacca gatggttact ccattttatt cttaggagga
     1141 ggggcttcct tacaattctg tatggttcct ttcaacctgc tcaacaaaaa agctgcatac
     1201 cttaacacag gtgtttgggc cagcaaagcc atgaaagagg ctaaattatt tggcgaagtg
     1261 gttgaagtag cttcatctaa ggctgataac tatacttata tacccaaaaa ctttacagta
     1321 cctgaagatg ttgattattt tcatgttaca accaataaca ctatttatgg aactgaattg
     1381 aagacagatt tgagtgtaaa tgtacctttg gttgcagatg cttcttcaga tattttgagc
     1441 cgtccgatgg atgtatcaaa atacggtttg atatatggag gtgcacaaaa gaatttaggg
     1501 cctgccgggg ttacctttgt tattgttaag aatgatattc ttggcaaggt aacacgccca
     1561 attcctacta tgttggatta caaaacacat attgaaaacg attcattgtt taatacccct
     1621 cctgcatttg gaatttttgc cattcttcag actttgaagt ggattaaatc tattggaggc
     1681 atcaatgcta tttataaaat gaaccttgaa aaagcagctc ttttgtacga agaaattgac
     1741 aggaacccac ttttcagatg tccggtatca gtagcagaag accgttcaat tatgaacgtt
     1801 tgttttgtaa tgaacgaaca atacaaggag cttgaaaacg aattccttga actgacaaaa
     1861 actaaaggca ttgttggtat taaaggccac aggtctgttg gaggtttcag ggcttcaaca
     1921 tataatgctt tgccaaaggc aagtgtagag gtccttgttg ctgcaatgca agaatttgct
     1981 aaacaaaaag cgtaattaac tttaagttta aaaaaagatg aagaaagtac ttgttgctac
     2041 agaaaagcct tttgcaaagg ctgctgtaga aagcataaaa caaatagtag aaggtgctgg
     2101 ctatgaattt aagacttttg agaaatatac cactcaggaa gaattgattc aggccgtagc
     2161 tgatgcaaat gcattaatta tccgcagcga cattgttgac agcaaagtta tagaggctgc
     2221 aaaaaacctt gagattgttg tacgagctgg tgcaggctat gacaatattg atttagcagc
     2281 ttgttctaaa cgtggtattg tggcaatgaa tactcctggt caaaatgcca acgctgttgc
     2341 cgaactggtt tttggtatgg tgatttacct tatccgcaac caatttgacg gttctacagg
     2401 ggttgaactg cgaaacaaaa agctgggtat ccatggatac gggtatattg gcaagattgt
     2461 agcccttatt gccaaaggct ttggtatgaa agtatatgct tatgacccat ttgttgacag
     2521 ggtccttata gagaatgatg gggttaagta tgagtcaaaa ttagaagatt tatataccaa
     2581 atgtcagtac atttcattgc atgtgccagc taatgaaaag accaagaaat caattaatta
     2641 cgacttattg aagttgatgc ctgaaggagc ttgccttatc aatacagccc gcaaggaaat
     2701 tattgatgaa gaaggtatgt tgaaattgtt tgaagaaaga aaagacatta aatatgcttc
     2761 agatattcag cctgactgtg cagctgtttt tgctgagaaa tacaaaggaa gatattactt
     2821 cactcctaag aaaaccgggg cacaaacaca cgaagcaaac aacaatgccg ggttagctgc
     2881 tgctaaccaa attgtaaatt tcttcaaaaa cggggataaa acattccagg ttaacaaata
     2941 agatatgatg gaataaaaga gatgccgggt aattccggca ttttttttat ttttattcaa
     3001 cacaaaggca cgaaggctca aaggataagg taaatgtttt ataccatcat tagttttttg
     3061 tgatacaatt atttggagat aaatttgtta taaaatattc aaaacactgc aattatgaag
     3121 tttgtagtca ttgaagggct tgatggaagc ggcaaatcaa cacaggttgg gttgctgaag
     3181 gaatatttga aaaagaataa catttcgttt caatacattc attttccaag ggttgagacc
     3241 gggatatatg gagacatggt agcaaggttt ctcagggggg agtttggtaa caacgaccag
     3301 gtaaatcctt atctggtagc attgttgtac gccggcgacc gtatggatgc tgctggacaa
     3361 atcaatcaat ggcttgccaa tgatgagctg gtgcttttgg accgatatgt tatttcaaat
     3421 attgcttatc aatgtgctaa agtggtagat aaagctgaga aggaaaagct gaaaaactgg
     3481 attttacagc tcgaatatga gcataacaag atacctgtcc ctactatcaa catttttctg
     3541 gatgtgccat tcgattttac ccgtgaacgc ctgacacagg gacggacagg cgatgaccgc
     3601 gactatttga acggggctac agatattcat gaggctgatt tgaacttcca ggaaaaggtg
     3661 cgtgaggttt accttgaatt tggcaaagat ggcaaggctt tgaagattat ttcgtgcaac
     3721 gaaaacggaa aaatgttgcc cccggatatc atttttgaaa agatattaaa tattcttaaa
     3781 aatgaaaatg tactgtagat tggatattta ttgtgataaa tttgaataaa caaaagcacc
     3841 caacccctct ttacaaggag gggaaatatt tcactaaaaa ggggagttgt tatttaagaa
     3901 ataaaaaatg taatgctatg gaacgatttg taaaatacct gtttcatttt tgccggataa
     3961 gcattggact attatttatc ttttcgagtt ttaccaaagg gcttgaccct tggggctttg
     4021 catacaagct cacagattac ttccaggtca tcaatatcaa taatttgcat tattctacac
     4081 ttgctttgtc ttttattgca agtggtttgg aatttttact tggtgtaatg cttttattta
     4141 atgtgctgat gaaatataca gcctgggcag cagcagcttt tatggcattt tttacagttt
     4201 taacttttct gctggcttgg tttaacccgg tttcggactg tggatgtttt ggcgatgcca
     4261 taaaacttac caattgggaa acatttttca aaaatgttgt attttctgtg cttgtagcag
     4321 ttatattttg gaaaaggaat aaatataaag tttctaaaaa tacttatttg acttatggcc
     4381 ttataacgag cggtattggt gtgtttgtat tattttcagt ggcatcatac aggcatcttc
     4441 ctatcataga ttttatgcca tacagtatag gttctaatat caaagaaaaa atgactgttc
     4501 ctccgggtgc ccctgttgat aaatatgaaa cgatgcttac ctatcagaat aaaaaaacag
     4561 gggagaagaa agattttgat ttgaagaact atccctggca ggattcattg acttggaaat
     4621 gggtagagac taaaagtgtt ttggttgaaa aaggatatac ccctccaatc catgattttg
     4681 ttatctcaga ccatgaaggt aatgatataa cccagcaaat attgaatgat tcagggtatg
     4741 tatttctgtt tatatcgtat aatttaaaat tggccaatgc ggctgctttg aaaaatgcag
     4801 attcgttgta tttatattat tcagggaaac ctgataccag gttttatgct gtaacagcat
     4861 caactgttgg ggtgcaaagc cagataaaag actcgttagg tttgaatatg gctttcaaca
     4921 ccggagacga aactatgttg aagacggtga tacgctctaa tcccgggttg gtattgttaa
     4981 agaacggcac tgtattagga aagtggcatt ataatgatat tatctgccaa gacttttttc
     5041 aacatcctga tttacttgct tattcagttc ataagctgaa caggggcaat gaagtgatgt
     5101 ttaccgcttt gatttttgct tttttgagca tcctgatcct ttttctttat tattataaca
     5161 aaaagtagtt aagcagaagt attgaaaaca cttattttat acattcttaa aaaagcgctt
     5221 tattcggttt caatactgtg gggggtaatg accctcatat tcattctgtt taatgtaatc
     5281 ccgggagatc cggcacgtat ggcacttgga caacgctcag atagtgcatc attggctgct
     5341 gtgcgtgcag acttaggact tgacaagcct gtaacgatgc agtatgtaaa gtatctcaat
     5401 gatctgtcgc ctgtcagtgt tttcaactgt aaagaccagt ccagttactt cttttacgat
     5461 acaactgttt ataagcattc tgcaaagctt tttaacattg gagaaagtaa aggcctagta
     5521 ttgaaagccc cttatatgag gcgctcgtac caaaacaagc gttttgtgtc tgatatgata
     5581 agtgaagcat tagttccaac ttttgtatta gcttttatat caatagtttt ggccacaata
     5641 atagggatag ggttaggaat actatcggct ttgaaaaaag atacctgggt ggacaaggta
     5701 tctattgttg tcagtgcatt gggtatgtct ttaccctctt attttgctgc tattctgttt
     5761 gcctggcttt ttgcttacct gttgggcaat gtaacaggcc ttgatttgac aggcagcctg
     5821 tatgttatag acgacatggg cgaagggcgg catctggcat tgaaaaatat tattcttcct
     5881 gcattgacat tgagcattcg acctttgtca gtatttacgc agcttacacg aaactctatg
     5941 ctggacaccc tgtcgcaaga ctatatcagg acagcacgtg ccaaggggct ttctcaatat
     6001 tcggttgtga tgcaccatgc tttgaaaaac gctttaaacc ctgttgtttc atccatatcg
     6061 ggctggtttg cctcgatgat ggccggggtg atatttattg agtatatttt tgcctggaaa
     6121 ggcttaggat atgtgatggt caatgccctt caaaactttg atgtcccggt agttatggga
     6181 acggttgtcg tcatctctgt tattttcatt gtggtcaata tctttaccga tattgtttat
     6241 tccattttgg acccacggat aagcctcaaa taattctaca tatttccaaa ctgtaaaaag
     6301 gctacttcgt atatattgtt ttgatttagt tttatttact ttagcattct acactaattg
     6361 acgaaaaaat gaaaaaagtt ttgctaacag caattttcgg atttttcatg tatgatattt
     6421 ttgcctgggg catagatatt tacaaagtga aaaatgcccc tgttattgat ggtaatgcca
     6481 ttgatgcaac ctgggccttg caacccctta atgatattga acaagctttg ggcagtactg
     6541 atggttatag tggcagtttt aaaatgtgct atgatgacaa aaatatttat ttattgttat
     6601 acattacaga tcctacgccc aatcaggtgg gttcatctac atggcaaagc gattgcgtgg
     6661 agttattttt tgctatggat gtaaagaatt cagatacata tcggcccggc gattggcaaa
     6721 tcaggaagtt ggcttctaaa actcaagctg cagggggggg tagatggtag ttcaaatatt
     6781 gctactttgc tggcggatgc caattttaaa gtagtacaaa aagatggggg cccaatggtc
     6841 caggaatggc agattccaat aaatacgttg gtagaaacag ccaatttcaa tggatcatcc
     6901 tttcgttttg atattcaatt atctgataat gacgggactg gaaatgaacg taccgggcat
     6961 atttattgga actcgactgc tgatgaccaa tggaccagga ttcaaaatca gggtatgata
     7021 tatttgaaag ctgaacaacc cagcttagaa gtgcctattg aaatgggaag tacctggcgt
     7081 tttttcaaag ggaaaaccgc tccttcggta gaatggataa accctagctt tgatgattta
     7141 tcatggactg catcttctgc accatttagg tttggagatg gtactggagg aatagagctt
     7201 acagatatgc tcaacaacta taccagcatt tttctgcgaa agacatttac tgtcaatatg
     7261 cctgacagct tgattagtga atgcaatgca acaatggtca ttgatgatgg gtacaagatt
     7321 tggttgaatg gtaaagaaat aacgagttac aatattcctt tgtcagtgaa gtataattca
     7381 ttggcctcat ccgctgttga aatgcaaact atttcaaata gtttcaaaat agaagatgtt
     7441 gggttgaaaa atggacaaaa tactatttgt gtccaggtgc tgaattgcag tcttaccagt
     7501 tcggacttat actttgacat gatgctgaca ttgaacaaaa aactgcctaa agccccggaa
     7561 gtcaaattta gtaaacccgg aggttactat aatgcaaaat ttaacgttac attgagcggc
     7621 ggacaagatg gggatacaat caaatataca ttagacagct cagatccgca aacctcttct
     7681 actgctatac ttgctgtttc gccagttact attgtcattg atccggatga taatacgcgg
     7741 atagttaaaa caccgggcat tgtagtacgt gctgttgtgc aaaaaactgg ttataactcg
     7801 ggaaaccctg aaagcaggac ttatttattt ttagaaaaag ttaaaactca agtttatccg
     7861 ggtggaacct ggccatcaag tgctgtaaac aaccaaataa tggattacga tgtaaaccaa
     7921 agtgtagtgt cccagtattc atcagacttt tcaaaagctt ttactaccat cccgacggtt
     7981 tgtctggtta ccgacaatat tggacttttt gatgcttcac aagggatgta tgtaaatgcc
     8041 ctgaaccatg gcgaatcgtg ggaaaaggct gcttcaatag agctaataaa cactgataat
     8101 acagaggctt ttgcttcggg cataggattg cgtatcaggg gcgggtggag ccgtcatccg
     8161 gataacccta aacatgcatt cagggtattt tttaaagata aatatggaaa gaaaaagttg
     8221 aaatatccca tatttggaga agatgcagcc cagacttttg ataagtttga tttgcgttgt
     8281 gcacagaatt attcatggag ctattatcac gacaatatgt tgacttatgc ccaggatgaa
     8341 acgtgtagag atattcaggg cttaatgggg catacttata cccgttcaag gtattgtcaa
     8401 ttgtttctga atggtatgta ctggggttta tatgaattta tggaacgtcc tgaagcaaac
     8461 tttgccgagt cgtacaaggg aggcgacaaa gatgattatg acgtgatcaa agttgctaca
     8521 gataatagca gtaatctgga agccaccgat ggtaatatgg atgcttacca gagattgtat
     8581 tcgtttacac aaagtggttt tacagcagat gcagcttatt ataaattgca agggcttgat
     8641 aagaccggga atattgatac cactcttgag aaacttgtgg acatagacaa cctgattgat
     8701 tatatgctga atatctttta ttctggcaat tttgatgcac cattgtctga atttggcggt
     8761 aataaccaac caaataactt ttatgctatt aaaaacagaa attccaaaag ggaaggtttc
     8821 tttttcattg ttcatgatgc agaacatact tttaattata tctcaggaag tcaggaaaat
     8881 aataattcgg gggttcaaga aaatcgagtg agcttagaaa atgatgggat gagctcacca
     8941 agttcatatt taaagtttac gccacagtgg ttgcaccatc gtttaaaaag caatgccaaa
     9001 tatcgtctgc gttttgccga cagggctgtt aaatatttgt ataacgatgg tttgcttact
     9061 gcttctcagg ttgagaaagt gtttaaagcc cgcacaaacc agataaatat ggccatcatt
     9121 ggagagtctg cccgttgggg cgattcaaaa tcttcgtatg ataattcaca tacaaaagat
     9181 aatgcctgga ttccggctgt aaacaatacg gtgtcgaaat ttattaaact tcgcacccca
     9241 attgtaattc agcaattgaa agatgccggc ctgttgccca aaacagatat ccctgtggtt
     9301 aagcaagatg ggaaagccgt atctgtaaat aaggttgaaa taagcaaacc aataaaagtg
     9361 acattcgaaa atccaaatgt taatggaaaa attgtagtat ctacagatgg caatgacccg
     9421 atgaatattg acgggacgca atattccagt gcaacaactt ttgcaaatgg agaagaaatg
     9481 gacatttcgt ctgcaactat tgtaaaggcc cgcattaaaa ccgataccga atggagccct
     9541 ttgcgtgaag ttatttttac gaataaagcc aacagggaaa atatccgtat tactgaaatt
     9601 caatacaatc cggctgctac agcatctttt gggtcaaagt ctatggaatt tattgaattt
     9661 aaaaatattg gaacaacagg cattgatttg agtggttgcc ggatagatag tgctgtaaaa
     9721 tatactttcc cggaaggctc aatcattgct cctggcaaat ttgttgtcat tgctgcggat
     9781 atcactgcgt ttgagtcaac ctatctgaaa gcccctacgg gcaggttcag tgggaacctt
     9841 gccaacgaag gcgagcgtgt tattttgtat gatgaatcta atactgcttt aattgacttt
     9901 acttacaagc ctgattgggt gttaagggct aatgaaacag gtttttcact tgtagccaaa
     9961 agcaaaaccc cagatacaaa accagaagca ggcacaacgt attggaaagg ttcagtttta
    10021 attggaggtt cgccatttgc tgatgatgaa ggctatattc cgccaagtga aatagagacc
    10081 tcaaaaataa cttgtagcat ttaccctaac cctgcgtctt ctgtaattaa aatcgaagct
    10141 atgggcagta atttcaggaa tttaaaaatg gtagaccttc aaggccgcac tatttataat
    10201 gaaaacctga ggtctgacag gataagtatg tcattaaacc ttcaacaact tcaagtttct
    10261 caaggtattt attttattgt attaaaaaat gataaagaaa tgaaggtgca aaaggtggtt
    10321 gttaagtaat atttagcttt acaataacta tagcaacaat gttttgggat aataaaataa
    10381 aataccccaa aggctttata tagctttggg gcattttatt tcagctcttt gtactgatta
    10441 ttcaagcgcg tagctttcag tcatttggtt ataaaacttc acgaagtcat acttctcagg
    10501 tgattccatc cattccatgg cactctttgg gtgttcgttt aacatacctg tgataaggta
    10561 aggaatgtgg tacccccaat caatcttttg ttgtaaaggg ataatctgtt cctggataac
    10621 tttaataatt gggcgaagtt tgaactttgg gtttttcaaa aaagctataa gggtttcggt
    10681 atggcaatta ccagctccac gtccaaaacc tagcaatgta gaatcgagca tgttgcaacc
    10741 ttcaataatg gctgtgatag tattggaaaa agccagctgc atattgttgt gagcatggat
    10801 accaatggtt ttacccggca atgccttgaa atacttacga gctaacagct ctatttgttc
    10861 gcaatacata ctgccaaagc tgtccactac atacaaagtt ggaacacgcg atttagccac
    10921 atcgttcagc gcttcatcca ggtcgcgttc gttaacctta gatacagcca tcaggttgat
    10981 agtggtttca tagcctttgt ccatacaatg ttcggcaagg gcaatagctt tgtccatttg
    11041 gtgaacataa caggccacgc gtatcatgtc aataacgcta tcctttttag gagcaatatc
    11101 tgtaggttca atacgtccaa tatctgccat gcacgaaagt ttcaggttgg ttttgttatc
    11161 acccacaata cggcgcaaat ccttttcatc acagaatttc caaggtccat tttcggtacg
    11221 cgaatacgat ttttcgctgc ttttgtaccc aatttccata taatcgaccc cggcttcgac
    11281 cagtgcatgg taaaccgctt taacaaattc gtctgaaaac tgccagttgt tcatcaatcc
    11341 gccatcacgt atcgtgcagt ctatcacttt aatttctttt ctgtacatgt gtttagtgtt
    11401 aagttattat ctatttaatt aaattctttg catactctgc ttgttcgcgt atataatatt
    11461 caataagctc tctatatact ttttcaattg aattggcatc aagtccgagg tttgaagccc
    11521 attgaccacg ttgtttgatg acattttcaa aacgcacgcg gtctgcaact tcaggttggg
    11581 ctggatttct aaatttgact acagctttta cgtattcaaa acggtcggca aaaagttgaa
    11641 taatttgctg gtcgatggag tctatttctt ttctgacttc ttctaaagaa aggcattggt
    11701 aggctttttt ggtcatggct ttaattaatt ctggtttaat tttcggagtc aaaaatatac
    11761 atttttacct aacattcttt caaatttgaa aatatattta cacttaaaac aggggcttta
    11821 ttatttaatt tattttataa ataattgtaa tataattaat tatgaatata aattaattta
    11881 aaatgtaaat taaactttac atttattttt atttaaaaaa aattgaattt tatataataa
    11941 ttgaaatttt atttttactt ttgaaaaatc aaaatagaaa gatacttata aatgtttttt
    12001 cattttttaa agagagttta aaatattaat aattaaatac ttatcattat ggcagacaat
    12061 aaagtgttca tttttgacac caccttacgc gatggcgaac aagtgccggg ctgtcagctc
    12121 aataccattg aaaagattga agttgccaaa gctttagagg ctttaggtgt agatattatt
    12181 gaagcaggct tccccatctc aagcccagga gattttaact ctgtagtaga aatatcaaaa
    12241 gctgtttcag agccaactat ttgtggtttg acccgtgccg tgaaaaaaga tattgaggtt
    12301 gccgcagaag cgctcaaatt tgctaaaaga gcgcgtattc ataccggtat aggaacttca
    12361 ttttatcaca ttaattataa gctcaaatcg aaccctgaag aaattattga gcgtgcaaaa
    12421 gaagcagtaa aatatgcccg caaatttgtt gatgatgtgg aattttatgc tgaagatgct
    12481 ggccgtaccg agaatgaata tcttgcccgt gttatcgaag cggttattgc agccggggca
    12541 actgtggtaa atattcctga tactaccggc tattgtattc cggaagaata tggccgcaaa
    12601 atccgttttc tgaaagagaa tgtaaagaat atagataagg caatcatttc tacacactgc
    12661 cacaacgacc ttggtatggc tacagccaac actattgccg gtgtgatgaa tggtgcccgc
    12721 caggtagagg ttactatcaa tggtattggt gagcgagccg gaaatacatc tttggaagaa
    12781 gtggccatga ttttgaaatg tcgcaacgat attaagctta acacaggtat caataccaaa
    12841 aagattattt ctacaagccg tttggtttcg acattgatgc gtatgcctgt acaagctaac
    12901 aaagctattg ttggacgtaa tgcatttgcc cactcatcag gtatccatca ggatggtgta
    12961 ttgaaacacc gcgaaaatta tgaaattatc gatcctcgtg aagttggctt gagcgaatct
    13021 gctattaaac taacagcccg tagtggcaga gctgctttaa aacacaggtt tgaaaaactt
    13081 ggttataagg ttgacggcga taaattagat aagatttacg aacaattctt agctatggcc
    13141 gataagaaaa aggatatcaa tgatgatgat ttggtattgt tattaggtga agaccgatca
    13201 aaagacaaga ttatccaaat ggagtatttg aatgtattgt gtggcaaaag cgcaatacca
    13261 atggctactg ttaaacttaa atataatgga gaagagcatg tagcaacttc atctggtgta
    13321 ggcccggttg atgctgcctt ttctgctgtt cgtcagattg tgaaaacaaa agctaagctt
    13381 gaagaatact tggtacaggc tattacgcgt ggaacagatg atattggcaa agttcacgtt
    13441 caggttgaat acaaaggcgt aaattattac gggtttgggg ccaattctga tgttattaca
    13501 gcttctgttg aagcatatat cgatgcaatt tctaactgta ttaaagcaaa tccaggaaaa
    13561 aattagtgtt tataaattta aaaattatga gtgcaaaaac attatttgac aaagtttggg
    13621 aacgtcacgt agttcatcag attgaaggcg gcccggctgt tttgtatatc gatcaacacc
    13681 tgattcacga agttaccagt ccggttgctt tcaaaggtct tgacagacgt ggtattaaag
    13741 ttcgtcgtcc tgacaggacc cttgctactc ctgaccacaa cattccaacc atgggccaag
    13801 aactgccaat taaggaagaa ttgtcacgta tgcaggtgca acaactttcc gaaaactgca
    13861 agaaacacaa cattacctta tttggtcttg gacacaaata caatggcgta gtacatatta
    13921 taggccctga gcttggtatt accaaacctg gaatgaccat tgtttgcggc gatagccaca
    13981 ctgcaaccca cggggctttt ggttctattg cttttggtat tggtacttct gaaattgaga
    14041 tggtacttgc ttctcaatgt ctttttcagg caaaaccaaa gtccatgcgc attaacgtaa
    14101 acggaaaact tggcaaaggc gtaacttcta aagatatcat cctttatatt attgcaaagc
    14161 ttacagctgg aggaggtaca ggatactttg ttgaatttgc cggtgatacc atccgttctt
    14221 tgagtatgga agcccgtatg acaatttgca acatggctat agaaatgggt gctcgtggag
    14281 gtattattgc tcctgacgaa actacatttg aatatatcaa aggccgcgaa tttgccccta
    14341 aaggtgaaga atgggataaa gctttggctt attggaagac tttgccaaca gatgaaggcg
    14401 caaaatttga tatagaatat agttttgatg ctgccgatat tgaacctatg attacttacg
    14461 gaacaaaccc gggtatgggc ttgaaaattt cgcagaatgt gccttctgat gaacccaata
    14521 aacaagcttt ggagtacatg gggctcaaag ccggcgactc gatgattggt aaaccaattg
    14581 attatgtttt tattggaagc tgtaccaatg gccgtataga agattttcgt gcttttgcga
    14641 gtgttgtaaa aggtaaaaag aaagccgata acgtaactgt ttggattgtt cccggctcaa
    14701 aacaggtaga acgtcaaatt gaggcagaag ggttgaaagc tattttcgaa gctgcaggaa
    14761 tgcaacttcg ccagcctggt tgctctgcat gtcttgctat gaacgaagac aaagtacctg
    14821 ccgggaaata tagcgtagca actacaaacc gcaactttga aggacgtcag ggaccaggat
    14881 cacgtacatt ccttgccagc ccgcttacag cagcagcctg tgccattacc ggcaaaatta
    14941 ctgacgtaag gactattatt taattagcca atgagagaat tagcaaatga aatatcctta
    15001 attggctaat tttcaaatta tcgaattttc aaattagtat tttatggaaa aatttcaaac
    15061 aataacatca agctgtgtac ctctgaatat cgaaaatgtt gacacagacc aaattatacc
    15121 tgcaaggttt ttaaaagcta catcacgcga aggttttgga gataaccttt tccgcgattg
    15181 gcgatatgac aacgaaaata aaccaaaacc aaattttgtg ctcaataacc cggcttactc
    15241 gggaaaaata cttgttgctg ccaaaaattt tggttgtggg tcaagtcgcg aacatgcggt
    15301 atgggccatt tacgactacg gttttcgggc tgtagtatca agcttcttcg cagatatatt
    15361 taagaacaac tcactaaact gcggtttact tcctgttcaa gtttctgatg ctttccttgc
    15421 taaaattttc aagcaagtag atgcagatgc taaaacattg cttaaaattg acctcgaaaa
    15481 ccaaaagatt actattgttg caacgggcga atctgaacct tttgaggtaa attcatataa
    15541 aaaagagtgc cttttgaaag ggtatgatga tgtagattat ttattgagcc taaaagataa
    15601 aattgaggct tacgaaaaag ctcattaatt ggtcttaata attgactcaa caaaaacttt
    15661 ccggcgataa gcatgccgga aagtttttgt tataattaaa aatactataa acataaaccc
    15721 ttttccaatg gtttataaag aagtgctaaa tttgcacaag tagtatcatc taaaaatgaa
    15781 tagaacttag aatggctaaa gtgatagaaa taatggacac atcgcttcgc gatggagagc
    15841 aaacaacagg cgtgtcatat tcggatagcg aaaaacttaa tattgccaag gttttactgg
    15901 acgaggttcg cgttgaccgg ttggaagtag cctctgctcg tgtatctgaa ggtgaataca
    15961 aaggcgttca aactattact tcctgggccc ggaaagctgg ccatatcaac aagattgagg
    16021 ttcttggatt tgtagatggc ggaaagtccc tcgaatggat acaaaaagcc ggaggtaagg
    16081 tgattaattt acttacgaag ggtagtttaa aacattgcaa agaacaactt cgtaaaacac
    16141 ctgaagaaca ttttgccgac ataatacaaa ccattctttt ggccgaaaag atgggcatca
    16201 tggtcaacat atacctcgaa gactggagta atggcatgcg acattctaaa gagtacgtgt
    16261 tttctatgat tgatgcgttg aagaatcagc ctgtcaaacg ctatatgctt cctgacacac
    16321 ttggaatcct taatcctgat gaaacttttc aattctgtca tgaggtagtg tcgcgttatc
    16381 cgggtttaca ttttgatttt cacccccata acgactatga tttagctgcc ggcaatgttt
    16441 ttatggccgt aaaagctggc attcaaggta tccataccac agtaaacgga ttgggcgagc
    16501 gtgccggcaa tgtacctctt gccagtgttg tagctgtgct taacgaccac ttcaatgcaa
    16561 aacttcctgt agtagagtca agcctgaata aggtaagccg cttggtcgaa tccttctcgg
    16621 gtattcgtat ccctgtgaac aagcctattg tcggtgaata tgcctatacc caggtggcag
    16681 gtatccatgc tgatggcgac aaaaagaaca aactctatta taacgacttg catcccgaac
    16741 gctttggccg acaacgtaaa tacgcgcttg gcaagctcag tggcaaggct aatattgttc
    16801 aaaatcttga agaacttggt attgagctta ccgaagaaga aatgaaaatg gtcactgagc
    16861 gtatcattga gcttggcgac cgcaaagagt atgttacccg tgaagacttg ccttatatca
    16921 ttgcagatgt gttgcaaagc aatattgtta aggaaaaggt ttttatcaaa aatttttctc
    16981 tctctgtggc taaaggtttg cgttcggtag ctacgttgaa actttcaata gattgccatg
    17041 aatacgaaga atcggcttcg ggcgatggac aatacgatgc ttttatgaat gctttgataa
    17101 aaatttatca tggcctgaac aagcatatcc ccgaactgtt ggattatgta gtgtctatac
    17161 ctccgggagg ccgtaccgaa gctttggttg aagccatgat tacctggcgt attgatggca
    17221 aagaagtaaa aacacgcggt ctggactcag accagaatgt agcagccata aaagctacca
    17281 tgcgaatgct taatatgatt gaaaagtaaa tgcattctat aaaaaatatt gctgtaatag
    17341 gtattggcgg agttggcggc tatgttggcg gaaagctggc acagctttgt gcttctgatg
    17401 agtttcaagt taatgtatca tttatagccc gtggtgcgca cgggcaaaag attgctaatg
    17461 aaggattgac tttacatgta gctaaggaga cttttaaagt attgccaagc gaagtcattg
    17521 aaaatattag ccatctgaaa tctcccgatt tggttgtttt ggcaacaaaa agctatgatt
    17581 tagggcctgt tgccggacaa cttgataagg ttatccgaaa agatacgctc attatgccat
    17641 tgctcaatgg tatagatatt catcaaagaa ttcgtaaggt aaccagcaaa ggcatcattt
    17701 taccttcctg tatttatata gcttcgttta taaatgcacc gggagaagtt actgttttag
    17761 gggcaaaggt gttaatcaac accgggcata ccaatccttt tgagaaggtc aataaagaag
    17821 ctgttatttc attatttgaa aaagccggat taaaactgaa atggaacgaa aatccatttc
    17881 tggccatttg ggaaaaatat gcttttgtgg cctcttttgc tattgtaagc ggttgtacac
    17941 gccagtcttt tattgagatg gttaacgagc cttcatccaa tcaggcagtc agggatgtaa
    18001 tgagcgaaat tatctcgttg gcacgggcaa aagacattat tttaccagca gattttgttg
    18061 agaaatcaat ggagacagcc aaaaaacttt cgcctgatgc taaaacttca tttcttcggg
    18121 atatagagag cggtaaaggc cttaatgaag cagatatctt tggcgaaaca gtgataaggc
    18181 ttggaaaaga gcttggcatt tctacgcctg ttacatctaa aatggttcag ggtacaaagt
    18241 tttaaaataa tcattaatac ataaatttta attttttcaa aaaatgggaa aaacgtttaa
    18301 tgtagctgta atgtcgggag acggaattgg tcccgaagtt gttgctcagg ctgttaaagt
    18361 gttgaaagct gttgaaaaaa aatacagtgt atcgttcaac ttccgcgatg tgcttattgg
    18421 tgctattgcc attgaccaaa caggtaaccc tcttcctgat gaaaccatca gaatttgtaa
    18481 agaatctgat gcagtattgt taggagctgt tggtcatcct aagtttgaca acgatccatc
    18541 agcaaaagtt cgtcctgagc aaggtttact aaaaattcgt aaagaacttg gcttatatgc
    18601 caacattcgt cctgtaacta catattcttt actcaatgaa gcttctccaa tcaaaaatca
    18661 tcttttaaaa gatgtagata tggtggttta tcgcgaactt accggaggta tttattttgg
    18721 tgaaaaaggc cgtaaggatg aaggtaaaac agcttacgac aactgtattt ataccgaagc
    18781 tgaagtattg cgcatttccc acatggcatt caaaatggcc caaagccgca agaaaaagct
    18841 gaccatggtg gacaaagcca atgtgctcga aagcagccgt ctgtggcgcg aagtagtgaa
    18901 aaaacttgaa gctcaatatc ctgatgtaac agttgactac atgtttgtgg atgcagcagc
    18961 tatgaaactg gttacctggc ctgcatactt cgatgtaatc cttaccgaga acatgtttgg
    19021 cgatatttta accgacgaag cttctgtaat tacaggttct ataggtcttt taccatctgc
    19081 ttcaatgggt gacaaatatg ctttgtttga gcctattcac ggatcatatc cgcaagctgc
    19141 cggcaaaaac attgccaacc cctacgggac tatcttaagc gctgctatgc tttgcgaata
    19201 tttgggtttg gctgatgctg caaaagatat ccgcaatgcc gttaacaaag gcctcgaaca
    19261 aggcgttgtt actgaagata tcaacaaaac aaagaacaat accactactg aagtaggaga
    19321 ttttgtggct gccaatttgt aataatacta atcatcataa aaaggcccag gacaatgctc
    19381 tgggcctttt ttgtcccatc ataaaaattg ttttttaagg tcaaataact tgtccttaca
    19441 aatctttaaa tgaaattcga atactcatta aataaataaa ttttacgctg aagaggctga
    19501 atttcgctgg atgttttttg caatttcaag attcttttct tcggtaatat cataaaaaag
    19561 cagcaatgtt ttgttcattt ctggagaaat agccatgatt gagtaccatt tgtgggtatt
    19621 tgggctgtaa gcataaaagc tcgaaataga gccaaacctg ccaacatggc caattagtgc
    19681 cccccaattg aatttatttt ccttcgtttc aggaaaaata ttttcataaa gtaaagaagc
    19741 taaagaggta ttatctgtct ggaagagttt gtgaaaaccc tcatcagcat caatacattt
    19801 ataggtaaag gttttagtaa catcatcata agaaacatca tgcaatgccg cagaaatatt
    19861 atcgacatcg accgttttgt tgtatcggtc atgaatccat tggatgatat cccggtattt
    19921 tagaagacta tggttcatta cattatcaat agtatttaaa ggcttttgtt atatgctttt
    19981 tattgataaa taaaattaat tatttagaaa taaataaaca attgcactca gaaaaaatta
    20041 tcttttagat aaaatagagt tatacgaatt ataaatataa tatagttaac taatttaaat
    20101 ccaatttttt aatattttta aaaacgctaa aaagttattt ttttggcata aaatactcac
    20161 taaattatcg tattttccga agtgaaaatt gagcatattt tgttgttata aatggtacaa
    20221 atactgaaat atcaataatg gcaatagcct tatatttgcc atacaaattc tgccggagca
    20281 ggttcatggt ttgggggtta agtgtttttc atcctgcatt tcttcacaaa agataaccgg
    20341 cagaattttc tttttataaa aattccattt cttttatttc tatttttaat acatctcata
    20401 ttcaataact tttctgctta atttctgttg tcgctgttgc ggtgttattc ttttttgtaa
    20461 ctttataacc tatttaaaaa ccaaaaacgc aacaactatg aaactaagta caagtttatc
    20521 ttgcatagct tttgctatgt tcccgtttat gacaaatata atgggacaaa ctgctgctac
    20581 cgcagaaaca gccaatcctc ctgcagaaag ccaggtttct ttgggagaaa atgtaaaaat
    20641 gaaaattggt gggtttgccc gtgctgactt tttctatgat acccgcaaaa acgttgagat
    20701 attggatggt ttattggata tgtatccttt aaacaaaagt ttggatgcca acggaaaaga
    20761 tatcaatgat gtgtcgttac tacgcatgtc tgctgcggca tcccgcttga atgccaaatt
    20821 tacaggccct gattttctta acgccaaaac aagttcgctg gtggagtttg actttaccgg
    20881 ggtcaatggc atcggccttc gtttgcgcca tgcctgggta aagctcaact gggaaaagac
    20941 tgagctgttg tttggccgtt tttggcaccc aatgtttatc cttgatgcat ttcctacggt
    21001 gttggcttta agcacaggag ctccgttttg tgtgttcaac cgtgccgaac agttaaggtt
    21061 tacttacaat ttagggcaaa ttagttttat ggcagctgct gccggtcaga tggattatgg
    21121 atttagcagt gagtataact ctgctactgg cgctacttcg tatttgcata accagataat
    21181 gcctgatatt actgtaaatg ctcaattcaa aaatgataat attattttcg gggccagtgc
    21241 aaattataaa gtaaaccaac ctcgccttta tacacaggat gccagcactc cggctaataa
    21301 gtataaaact gacgaaaaaa tagcttcgct ttcagcgcag gcttatgcac aatacaaaac
    21361 aggtttgttg aaaatcaaag gcggtgccct ttatggcgaa aatatgatgg aactgcttat
    21421 gatgggcggg tatgcagtca aaacaattga cacggttaca tttaaagaaa cttattcaac
    21481 agtaaataac ctaaactatt ggtgcaacat tgtttatggg gacaaattgc aggcaatcct
    21541 ttttgttggt tacatgcaaa accttgggac cgttgataag caaattgtcg gttttacaaa
    21601 gaattctgct cgtggttatg atgaaaaagg aaacggtatt gccaacatga tacgtattgc
    21661 gccaggcgtt gcttataagg ttggcagggt tcaaatgcat ttggaactgg agcatagcat
    21721 tactgcttat ggaaaatata ccaatgctgc taatgttata aatgaaaatg cagaagtaaa
    21781 agacacaaaa aatatttcca atacacgggt acaatttgca actatctttt acttttaacc
    21841 cctgttttca cactaacaaa acttacaatg aatcagcaat attgggaaaa ggaattagag
    21901 acgttatcgc aggttgattt gcagatattg cagtacgagc gtttgaaaaa gacgcttcgg
    21961 tttgcctcac agtctatgta ttacaaaaag ttgtttgccg aaaaaaaaat taatccggac
    22021 ggttttaaaa gtattaagga tattgaaaaa atacctttca ctaccaaaga agatttgcgt
    22081 agtaattttc cttatggttt tctggcagcc gagaagcgtg aggttatccg tttgcactct
    22141 tcgagcggga caacaggaaa ccctacagtt gtatatcatt caaaacatga tattgactct
    22201 tgggcaaatc tcgtagctcg ttcgcttttt gccgttggga cccgagatac tgacgttttt
    22261 cagaatatta gcggctatgg cttgtttacc ggggggcttg gctttcagta tggagccgaa
    22321 aggcttgggg cgcttaccat tcctgcagga gccggaaaca gcaaaaggca gattaagctg
    22381 atgcgcgatt atggtactac ggtaatccat actatcccca gctatgttat gcatttggtc
    22441 gattttttca gggaaatgaa ccttgatcca cgtacagata ctgacctgcg tcacatggtt
    22501 attggtgctg agcctcatac agaggagacc cgtcaacgta tccaggcaat gatgggtatc
    22561 aaagcgtaca attcttatgg attgtctgaa atgaatggcc ctggtgtggc ttttgaatgt
    22621 gaatatcaaa atggtatgca cctttgggaa gatgcttttt atgccgaaat cattgacccg
    22681 gttactttac agcatgtacc cgaaggtgaa ataggggagt tggtacttac ttcgcttgac
    22741 agacaagcaa tgccaatact tcgttatcgt acacgcgacc tgacgaggtt tttgcccggc
    22801 agctgccctt gcggacgtac acatcgccgt atcgaccgta tgaaaggccg tagcgacgat
    22861 atgtttatca tccgcgggtg taatgtattt cctatgcaga ttgaaaaagt gctgatgcaa
    22921 attcctgaag tcggcaataa ttatatgatt cagctcgaaa ctattgatgg cgtagatgaa
    22981 atgattattg aagtggaagt attggatggc aattttcttg acaattattc aaaacttgaa
    23041 aaattgtcga aaattatcac caatgaagtg aaaaacgagg tgctggttac gccaaaagtg
    23101 aaactggtgc agccaaacca ctttcctcca tgcgaaggca aggctttgcg tgtaaaagac
    23161 aacagaaaac aatattaaac taatttgaaa attcgagaat gtgaaaatta gccaatgaaa
    23221 gaaattgaaa aatgtagttt cttcatttgc taattaccta atttgctaat taactaattg
    23281 aataaggtat gtcaatcaaa caattatcca tttttttgga aaataagagt ggaaggctca
    23341 cagaagtcct tgaaactctt ggacaggaga agattaacat cgcagcaatg agtattgccg
    23401 atacttctga atatggcata ttgcgactta ttgtatctga cccggaagaa gcgttacgac
    23461 ttttgaaaaa caaagggttt agtgttaaca tgaccgatgt tattgctatt gaaacgccta
    23521 cttatgcagg ggcttttgca gaggctttaa agcacttctc cgatgcccag gtgggtatag
    23581 aatatatgta tgctttcgaa ttagataata atggcattat agtgatgcgt accgatgaca
    23641 ggcaaaaagc ttttaaaatc atcgaaagca acaagcttcg tttggtgtcg ggcaatgata
    23701 tttttaaaga aaagaaataa tacctgattt aagattttat catgatttgg aataagactt
    23761 ttgagtgtat gagtcgcgag gagatgcgcg aatgccaaag caaaagactc atcgagactg
    23821 tacaacgtgt ttatcacaat gttcctgctt accgcgaaaa aatgcaacaa gcacacctcg
    23881 ttcctgagga tatcaggagt gttgatgatt tacataaact gccttttacc gttaaacaag
    23941 atttgcgtga caattatcct tatgggctat ttgctgttcc tatgagcgaa gtggtcaggg
    24001 tgcatgcatc gtccggaaca acgggaaagc caacagttgt aggctataca cgcaaggatt
    24061 tggctacctg gagtgaggta atagcccgta cattgactgc tgccggggca cacaaacatg
    24121 acgtaattca tattgcttat ggttatggcc ttttcactgg aggtttgggc cttcattatg
    24181 gtagtgaaaa gattggggcc acggttattc ctatttctgg aggtaacacg caacgtcaaa
    24241 tccaattgat gcacgacttt ggaagtactg tattggcttg taccccttct tatgctttat
    24301 atttggctga ggctattgac gaaagccaat ataaacgtag cgacctgaaa ctcagggttg
    24361 gtgttttcgg ggctgagcca tggacagaaa ctatgcgtaa ggagattgaa gaaaagctcc
    24421 atattaaagc tattgatatt tttggattga gcgaagttat tggccctggt gtatccagcg
    24481 aatgtgaaca ccaatgtggc ttgcatgttt ttgaagacca ttttatccct gaaattattg
    24541 acccaaaaac attggaagta ttgccagaag gcgaattggg cgagctggta tttacctgta
    24601 ttaccaaaga agcacttccg ctcattcgtt accgtacccg tgacctgacc cgcttgcatt
    24661 acgaaaaatg taaatgtggc cgtaccttgg tacgtatgga aaaatgtacc ggccgtagtg
    24721 atgatatgct tatcattcga ggggttaacg tttttccatc gcagatagag gaggtgctgc
    24781 ttaagattga cgggcttcaa cctcattatc tcattactgt tgacagggtt cataacctcg
    24841 atgttatgca gttgcaggta gaggtggagc aggaatattt tactgaccgc atcagcgaat
    24901 tgcaaggctt gagcaaaaaa ttgcaacaca gtcttgaaag tgctttgggt attggtgtcg
    24961 atgtgaagct ggtggagcct aaaaccatcc aacgtagcga aggcaaagct aaaagggtta
    25021 ttgataatag aaaattgaat taaataacaa taagtcacag atttaagccg aaacatattt
    25081 ttttgttccg gctttttttt atacattttc gccaactaaa cattattaac caaataaaaa
    25141 caaaacacta tgttaggaat caccgaccct tggattttgg cagcatacct gctgagcttt
    25201 ctaagtgctg tcgcctgtat tgtttatggt ttgtacaact ggaacaaagg cggcaacaat
    25261 gaagaatccc aaattacaga agaagtaaaa tgggaaaaag aagaaaaaca aattgaagaa
    25321 aatctttaaa aagaaaggag ctggctatgt tatttgacat tattgtggta ttaatatacc
    25381 tttttgtaat aacctatttg ggttatttag gttacaaaca caccaaaagc tcagcagatt
    25441 acttagttgg cggacgtaaa atccacccgc tgattatggc tttatcttat ggctcaacgt
    25501 ttatcagtac ttcagctata gttggatttg gcggtgctgc cggaacattg gggatgggtt
    25561 tgctttggct tactttcctc aatattttta tgggcatttt cattgctttt attgtattcg
    25621 gaaagcgtac acgccaaatg ggtcataacc tcgatgccca tacatttccc gaattgttgg
    25681 caacacgttt taaatcaaaa tttatacaag gttttgccgg cggtattatc tttcttttca
    25741 tgcctattta tgctgcagct gtgttaaaag gcggttcaga ttatattcag gcacattttg
    25801 gcatttcgta tgaagtttca cttttctttt ttgcaaccat tgtagccata tatgtatgga
    25861 tgggtggtat caaaggcgtt atgtacaccg agactttcca aggggctatt atgtttatcg
    25921 ggatgatatt cctgttggta tattcttatg cttcgttagg tggtattacc gaggcccata
    25981 ccaagcttac caacctcttt ggacatgccg aggtgcaaga ccaggctgct aagttggtca
    26041 aaggtggttt tatgggctgg acatcaatgc ctgcatttgg ttcgcctatc tggtggactt
    26101 tggtgaccac tattgtaatg ggtgttggaa ttggtgtatt ggcccaacct cagctggtat
    26161 tgcgttttat gactgtaaag agtaaccgcg agcttaaccg tgctgttatt tccggaggta
    26221 tatttattat tatgatgact ggtgtggctt ttattgtagg ggcattatca aacgttttct
    26281 ttttcgaaag tacaggtaaa atatctgttg tagctgccgg gggcaatgac aaaattatcc
    26341 cggtatttat caagcaatat ctgccaacat ggtatggggc tatttttctt ttgacgctga
    26401 ttgccgctgc catgagtaca ctaagcagcc agtttcatgc tatgggttct gccgcaggtc
    26461 gcgatgtgta tcaaaaagcg tttaataaaa aaggaaatcc gctgtttata tcacgtacag
    26521 gggttttggt tggtatcctt attagtgttg ttattgctta cttttcaggg aaattggatg
    26581 tgagtatgca gattattgct actggcacag cgcttttctt tggtttatgt gcagcatcat
    26641 ttttacctgc ttatgtttct gctttatata tccgcaaaat atctacaaaa gctgttattt
    26701 cagggatgat agctggtttt gtcgttggtg tgttctggat tttctttatt cacgaaaaag
    26761 aagcctcaag cttgcaatta tgtaacctgt tgtttgataa accttcgctg gtaaaagaaa
    26821 cagcactggc taaactggca cttgttgacc ctattatcat agcgcttcct gtttcaacac
    26881 ttgttacagc aattgtgggg gttacagcta aaagtaaact ggatgaagcc catctggaca
    26941 attgttttaa aggaataaaa taggcacgtt ttatttaaaa agtttaaaat actaaataca
    27001 tttatagccc gaaattcatg acaaggtttc gggctatttt ttttagcact aacaattgtc
    27061 aactaataac taacatctaa attttatgtc aataacacaa ttattactta ccttattacc
    27121 aatagcagta atattggtaa tgctcattgt attcagaaaa gctgcggata tcagtggtat
    27181 tgcaggttgg ctggctgttt cactggttgc ctttttcttt ttcaacacct caatagaagt
    27241 tttattgcgg tcaaccgggg cagggcttat ccgttcgttc tcggtatcgc tcattgtagc
    27301 cacatctttg ctgcaaatgg catttatgga aaaaacagga gcactgaaac gtatcatcat
    27361 ttttgtaaag accatctcga gcgagagccg tgccattcag attatgctta taaacattgg
    27421 ttttgggact ttgatggtgg ccgttggggc aactccggta tcgctattgc ctccaattct
    27481 tgtggctatg ggttattcaa cttacgtagc tattgccctg cctgcaattg gatacgattc
    27541 gctttgtact tatgcattat taggggctcc tattgtagtg tttgtcgatg tggccaataa
    27601 tttcttgggc ccgggcaatg aaatcagcct tcaccaggca gggatggtgt tttacatgtt
    27661 cttgcctgta gtatcaacac ttattggctt ctgtatgttg tggattgtag gtaaatggca
    27721 ggctatcaaa gaaggatggg tcccatgtct ggtgacaggc ctgactattg gcgtggtagc
    27781 ttattttact aaccgttatg acaacctggt tgtgcttacc ggcgtgatgt gcggcattgc
    27841 agttattgct atgatggtgc tattcctaaa agtgaaagga atgaaggtga ttgacaaaag
    27901 ccgccttaca cctgaggagc tcgaatatga aaagtcttat cctttgtgga aagcgctaat
    27961 gccatggctt atacttattg ggctcatcct gatgctgaat ttgcctaaag atgtgttcaa
    28021 ctatctttat agaacgctca aattacctgt tacaggatta tgtgctgatg gaaaacccct
    28081 tgatactcgt tttttgtggc aggcttatac ctggattttt gtaagtgttg tgctggctat
    28141 accatttctt aaacccacaa aagcccaaat gaaagacact ttaaaggtat ggtggaagcg
    28201 tgccccgcgt ccggtttttg ctgcggccat tttctttgct attggcgaga ttatgaatat
    28261 gtcaggctat agcattgtgg acaagggttt tgttgtaccc agcatgataa aggtactggc
    28321 tacttattct gcagatttct tccattcggc ttatggcgct gtggttactt ttataggctt
    28381 gtttggtggt tttattactg gtagcgaggc ttctactata gctatgtttg ccaagtattc
    28441 acttactacg gctaaagaac ttgcacttcc tgtaaccgga cttatcatta tcactgccgg
    28501 attggcattt ggaggagggt tggccagcgt tatttcaccg gccaaattgc aaaatgcagc
    28561 tgcttctatt gataaaatag gcgaagaaaa taaggttatc cgcatagcct ttgtttttgc
    28621 acttatcctg acaacggtta cctcgctgtt tactgtgctg ttgctgttgt tgaattgcta
    28681 agttgcttaa tatttattaa gatatgttat aagcctctga agaatagatc tcttcggagg
    28741 ttttatattt aaattttgaa acatgcagat acagtataaa atattaaaga gaacttttat
    28801 ccctcagatt atattgagtg ttattattgt attgataatt agaggtttta tttactatac
    28861 ccataaacct attgatacgt tttattttgt atgtatttgt gtgatgataa tttctaatgt
    28921 atttatattt taccctaatg tgaagaaaat tggccttttg cagtttgaca ttttgggagt
    28981 gacagtaaat atgaataatt cagctatccg atataatttt ttagatgaaa atattaagtc
    29041 cttaaatata aagctgaaaa tcaataaaaa taggctccat acaaaatacc ctaacaatag
    29101 tgcagttaat gcgctaaata agcttgaaat attttataat gaccagataa tcaagtataa
    29161 ctttaaagca gatagtgttg atgatcttaa aaaattgaaa gaaatgcttg ttaaaatcaa
    29221 tgagaaggca aaaatacttt taaattaagt taaactgtta cgaacttcgg tttacatttt
    29281 ttgagtattt gttttatctt tacaaaaagt ataaagatta tgacagtcac catgttatat
    29341 acaaaaataa gctctttgcc gccttcaatg attaaggaag tg
//
LOCUS       DAOSPF010000044        29319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1597_length_29319_cov_7.370387, whole genome shotgun sequence.
ACCESSION   DAOSPF010000044 DAOSPF010000000
VERSION     DAOSPF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29319
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_29319_cov_7.370387"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..3382)
                     /locus_tag="PK252_09540"
     CDS             complement(236..3382)
                     /locus_tag="PK252_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT14989.1"
                     /translation="MLKKLTLTIITALSCICISNYAQNEWETPELVSINAETPHVLFY
                     PYADAKSALTRNIKNSPFYISLNGKWKFNLANNPDSRPKDFFQPTFNSSDWKDIEVPS
                     DWQFQGYDIPVYVNIRYPFPANPPMVPSDYNPVGSYIKKINIPAGWKEREVFFQAGAA
                     NSMIYLWVNGKYVGMSKDSKTPAEFRISKYIQAGTENTIAVQVFRWCDGSYLEDQDFF
                     RLSGLERDVCLYSTPKVQLSDIEIISNLDDSYKNGLFKSRIFIKNYTGKPDKDYQIEA
                     TLINNEGKNAASPKTFKVNINDTAIVEWKEKIVNVKTWTAETPDLYTVLVVLKDKKGN
                     VLEAITQRTGFRKIEIKDGDLLVNGVRVLIKGVNRHEHDMVKGHVISEESMRRDISLM
                     KQFNINTVRTSHYPNDPLWYDLCDEYGLYVIDEANIESHGWHQWDEQTLAKNKQWLAA
                     HIDRTKRMFERDKNHPCIITWSLGNEAGNGSNFEATYAWLKERDKTRPVQYEQAGLSK
                     NTDIYCPMYPKIEHLKEYGSKKQDRPLIMCEYSHAMGNSNGNLKDYWDVIRATPHLQG
                     GCIWDWVDQSFASVNGKDTCFLYGGDFGMLFNIKSDSNFCCNGLVGSNRKPHPGLWEV
                     KKQYQSLHVKAIDLSAGKFSIVNEMDFTEMSKYDISWNILENGKPIAGANLGVQQLVP
                     HKSKEITVAYPLFERKEGAEYSIIFSYKTKVATAAVPKNFEVAWDQFILPFSKARTPF
                     DVNQLPKLNVKTNAKGMPSISGPNFNIVFDAQTASLVSYKFDTTEMIKMAPQADFWRA
                     PTDNDFGNKMPERLAIWKKATQNAKVDSFVLTPVNAWQVIVNTIFTLPEIKSKLYIKY
                     IIFGNGQVIFDYRFVPGKDLPDMPRVGLKMGIPAKYSYITMYGRGPHENYIDRNSGAK
                     LALHTTTVEDLFTQYVRPQECGNLTDVRWLALKDNLYNGLLIVGKEPLSISALNINTN
                     DLNYSYEKRHACDVSKTGYITVHIDKAQMGVGGDDSWGAMVHPQYLIPAKEYRYSFSI
                     KPFKVSEGNEETMMKKMY"
     gene            complement(3509..4192)
                     /locus_tag="PK252_09545"
     CDS             complement(3509..4192)
                     /locus_tag="PK252_09545"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOT14990.1"
                     /translation="MTRYFSKISVSVVFMSIFSLANIGFAQIVKKDTVQKRLSIGIDI
                     AKPVIYMIDKNKFEVEGSISYSNINNFNPIVEFGLENVELSQSDTIKTYEYQSDGKFF
                     RIGFDYNTFRRNLFGENNMVFFGFRYGYANQNHSAKNIIINDKFWPKRYTSLSTQEIQ
                     THWLELTSGIKVQFFKYFAAGWTVRFSYIIDQQNTSSVKPYYIPGIGKGAEKYNFGFT
                     YSLYYTFPL"
     gene            complement(4189..4731)
                     /locus_tag="PK252_09550"
     CDS             complement(4189..4731)
                     /locus_tag="PK252_09550"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOT14991.1"
                     /translation="MAKTIIYKFYGITIQMQYPLKYFILTIFLLLSFFSCTDSSDCFY
                     VSNRKVRLTFVTANGKTIKDTTISKFSIVTSDSLPLYDTASGQQIGLPLSQVADTSTF
                     YIAFDSIATDTLTFFYKRNLELISPECGFTTRFNLEHLKYSKNNITSVLFIDSYVDKD
                     IEYGVNYKIVIRKRTDFKKK"
     gene            4985..5824
                     /locus_tag="PK252_09555"
     CDS             4985..5824
                     /locus_tag="PK252_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOT14992.1"
                     /translation="MTIEDIDIQYFVTALKNSSGYDFSEYSEKSLKRRLAKVLMDNKM
                     ELNQLVTEIKKNVSFAEKVVKEITVNTTELFRDPFIWQMLRYKVLPTLQDNKIINIWH
                     AGCSTGQEVYSMMILLNELGMLEKARIFGTDINSDVLDIARKGIYKYRFNIGYLDNFD
                     KVIKENPYNYEKFNDIPYSKYFEIDKVKDFMRMHPFLTEKPTYKKHDLVRDENIFFNK
                     FDIILCRNVIIYFNYNLQNRVFNLFSNSLYQGGFLLLGMHETILGPMSASFDKKENIY
                     IKK"
     gene            5854..8517
                     /locus_tag="PK252_09560"
     CDS             5854..8517
                     /locus_tag="PK252_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT14993.1"
                     /translation="MKPVILTLKKKIAAILLLLLILVLVLFFTALNQFKIVRAHTSLY
                     QRANNLVYARQHIDSLQNQVQLALPVNLNFFKTGQSKLIDEIKDYNIKTIEEINKISN
                     DFYLKNTKSVNLKAFYISQILRNYNETLDKYTRLLYEKGFEQYGLVGQLKILTDYLMV
                     NFQQENNTALIAKLKEITQLKDKYLKEKDPVIISKISIITDEAKVQAIVSVKKDKATV
                     LLKLQKFEELIKQINQIDETIGYSPTEGVSGTLKIIADNYHNQAFLFKKIIERKIDNA
                     VKWAYTWIIAIFVLLVLTLALLFDTINKQIHKPLRKLTNFLEQLVKGKLPEKIRLKGN
                     DEIAVMARNLNDLTDGLRLKAEFAMEIGKRNQNTVFQPLSDDDILGNALIEMEKSLRK
                     ADLEDQKYKTEEKKRIWTNEGLARFGEILRSHNNDINVLADEVIQNLVKYLNAIQGAL
                     YFYNDDDPQNIHLDIAAAFAYDRKKFLKTSITLGEGLVGTVAVEKEKIFITDVPEDYF
                     TIKSGLGEAPPRSILIVPLKLEDEMLGVVELASYNIFQPHEIEFVEKIGMTIASTITN
                     VKINARTAQLLEQSQKQAEDMAEQEEEMRQNMEELRTTQEDFARREAELQGLFTAVKD
                     NALLLTIDKDGVIIEVNNNLINTLRSKPEDIIGRNYRDFSVLAKNGHEYDVFFNNLFN
                     GESQVIVEKIRLSNAKDLYLEQHFTPIADLHGVYTKAICFSYNITEQKILETKLELQK
                     KEIEASEQKLKDVFNIIDTSLLRCEYNGDCKIIDVNENYCRQTGQTKSEYIAKPGSIY
                     LKDDEKDQFDKIWNEVLKNKNYVGAVKRTKPTGEEFWLMAAFTPVCDASGKLLKVSMF
                     ALDITEKKLKYQLLEEANKEIERLKKASNDK"
     gene            8507..9361
                     /locus_tag="PK252_09565"
     CDS             8507..9361
                     /locus_tag="PK252_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOT14994.1"
                     /translation="MTNDNSYSLTSPIAAEIGIVEYRSVIRAISETYGYDFSEFALTS
                     MKRRIENFISAFNLRSVEALIDRLKNDKQYFQTFLKSISVESTEMFRDPSFWRTLRDQ
                     LLPLILSKVARPRVWFPTCVSGDELYTFCIVLSESNWLDKFEIIATYSNDLSLKQIQS
                     GQFDGHKYDVSSDNYERYQGNGKIRDHFISAEIKFQRNTSLIKNVHFIKQNVNFDNSP
                     TDVKLIIFRNQMLYFTQPLQDKVLTKLYNSLGQGGFIAIGVKEQMGMISSKYFRAINE
                     SENIFKKI"
     gene            9376..9939
                     /locus_tag="PK252_09570"
     CDS             9376..9939
                     /locus_tag="PK252_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOT14995.1"
                     /translation="MYKAVMIGGSAGSFQVVASILGSLPKNYELPIFLCLHRLKHVRT
                     GFVEVLSLKSAIPVIEPYDKEPIKPGRAYLAPANYHMYIELGNKFALSTEAPLNHSRP
                     SIDLSFVTAAQVYRNKLVGIILSGANKDGAYGLKKVAEYGGTTIVQDPNTCEVRTMTE
                     AALQMGNVNHIFSIDKIASFVANLKAI"
     gene            10101..10703
                     /locus_tag="PK252_09575"
     CDS             10101..10703
                     /locus_tag="PK252_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT14996.1"
                     /translation="MFFIIFKLVQVIIEEETTLQKILQQNEVLVENASTEDKKEEYKD
                     VNIDELVQKIIPAIPQNQSLDEFCGNLLTNLSKQLNIVQGIVYCKNNQTSQFELKSTY
                     ALYTTEPINPFFEGETLPGQVAADKKAICITDVPDNYFRIASGLGSAKPKHVVIFPII
                     DKEHTIAVVEIATFQKIEDNTFEIFNKLSAILGKILVKLK"
     gene            10715..13333
                     /locus_tag="PK252_09580"
     CDS             10715..13333
                     /locus_tag="PK252_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT14997.1"
                     /translation="MANTIKYKTWDPEIFYKSLVAGVAFVILPVSLWIVELALWQVPL
                     SLDSIIFIHQKSISLWVADLMPFTVGVLVYQIIIRRKKFADKLQTKIEEMQRQMDINA
                     KFAKEIGEGNYNSEFKLSESNDILATSLLLMRNNLLKNNKKEAEQNWIAEGKDEISNI
                     LRLHNKINDLAYEVLIKLINYIDAIQGAFYVYDDEKDKIVNVATYAYNRKKYLNQEFS
                     FGEGLVGECAFEMDIIYRTEIPDDYFSISSGLLGDRKPKSLLIVPLISDEKLQGVLEF
                     ASLKDEIPELTITFMKELGEIIARTIFNLRINQKTERLLAESQQMTEELQENEEQLRQ
                     NAEEMRATQEELQKSNEKLENQIKEVENAQKRINSLLENSSEIIMIYDEHLNLTYQSP
                     SVRKILGYLPEEMTHGKYFDRLTRKGEQEVRNMFRQLLENPLEVTTIQYTYIKKDGQK
                     IFIESSGRNLLNDPAIKGILLNSQDITERKRAEKEERMKSKMQSLSENSLDLILRLSL
                     QGHFFYANPIVEDYFGLEPKAVINKTVDEIPMDEALNEYFKVTLKQLRTKPEKRNQEI
                     KVPIKLGEKIIYRILNIDAIPEYNENELETVLFVGHDITEAKRIELEIQEKNKKIEDS
                     INYAQRIQSSILPDNRLIREFFPKSFIYYKPRDVVSGDFPWFFHHGDDIYIAAVDCTG
                     HGVPGAMLSFIGYFLLNNIVDNDKALNAGSICDMLHEGVRTTLKQDREDASGRDGMDI
                     AICKINVKKKELHFAGAHRPLYLVRKGELIEYKGDRKAIGGIPLGNKAEQPFTNYVIN
                     ILNGDRVFFFSDGLPDQLGGPEHKKYSPGRIRNLICENNSFSMSQYYELFVQDFEEWK
                     GESKQIDDVLLIGIEF"
     gene            13396..13974
                     /locus_tag="PK252_09585"
     CDS             13396..13974
                     /locus_tag="PK252_09585"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT14998.1"
                     /translation="MESIKKDSKGFLEFVYEFYKTMKAHEISLVYEGEITHQITKAFT
                     SLAESNMAKEEESGTVQRKVFHVMVECLQNISKHADDFADNDFLYSGRGIFMVSKGET
                     EYTVTTGNAIDNSKMKDMQEMLDRINSMDKEELNELYKKQIKEGRLSDKGGAGLGFID
                     IKRKTGKPLEYHFLPISENISFFLLTSSISRE"
     gene            13990..14367
                     /locus_tag="PK252_09590"
     CDS             13990..14367
                     /locus_tag="PK252_09590"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT14999.1"
                     /translation="MEAIKIKGTEDTPSVILDAAENIMEISGRSLPEDVSSFYGPILS
                     WLNEYAKSPNPKTVFVFKLVYFNTASSKLILDILMKLEELAKNGNEVLVKWLYPEDDE
                     DMQEAGSEYADIVEVPFEQIAYS"
     gene            14410..17493
                     /locus_tag="PK252_09595"
     CDS             14410..17493
                     /locus_tag="PK252_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15000.1"
                     /translation="MSEEILKALMQLFAIIAKQDEGVENTEKEYVRNFLSQQLSDELS
                     KEYYALFEKHAEWGDEEENTEGEKKKRLTKVNDSVRILGICRKINKTLTQNQKIVALV
                     RLFELVNADRKFSEQRMAIINTVSEVFNVGVEEFKSIEKFVIQNEPENIDDPLVLVIN
                     SKEKHDYACKHLHADNLDKNIFILHIPSVELYFLKYTGEQDLFLNGLAINNKRIYLFA
                     SGSSVKLPKAKPIYYSDVAAHFLADIHASKISFEVKDLEYKFSNGNIGLRNINFCESH
                     GRLVGIMGASGAGKTTLLNVLCGIEKPSSGEVLINGVNLHTQKKEIEGVIGLIPQDDL
                     LIEDLTVFENLYFSAKLCFKDKSEEELVQMVDNTLFNLGLLDRKDLRVGNALNKTISG
                     GQRKRLNIALELIREPSILFVDEPTSGLSSRDSENVMDLLRELALKGKLIFVVIHQPS
                     SEIYKMFDKMIILDTGGYQIYSGNPVEGVMYFKKIDAQINSEVGECPVCGNVNPELIF
                     NIVEAQVVDEFGNYTGVRKVAPHKWEEHYLNNFAKEKVDTINDAPPKSLNIPNWLNQL
                     KIYIKRDFLAKVSNKQYIILNLLEYPVLALMLSYIIRYIADPTSSVYIFRENENVSIY
                     IFMSIIVALFFGLTVSAEEIFHDQKILKREKFLNLSKSSYLLSKVLILFALSAIQTFT
                     FVLIGNSILGIKAMYFTYWLALFSIAAFANILGLNISASFNSVVTIYIIIPFLMIPMM
                     VLSGAMFSFDKLNRTVSSVDKVPLVAEVMPTKWAYEALMVHQFKDNLYERNFYEYEKE
                     ISNANFKLVYYIPELIKRLDKVMTELNTSPKVEKTKDDLLLLHNELLAQCQQVPFVEC
                     KIANCLNVDSFDMQASINTMEYINALNDYYQKVYDAADKRKNNMLQYLDEHKPQVYKY
                     YYDNFYNDHLTDMVKKAFEKEKNKLLEYDHRLIQQTDPIFLDPRVSHKFDFRAHLYAP
                     RKHFLGKYYDTYWFNIFVIWCFSVIFYLSLYFDLLKRLIDFPDKVRLHYRNKKVE"
     gene            17565..18428
                     /locus_tag="PK252_09600"
     CDS             17565..18428
                     /locus_tag="PK252_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15001.1"
                     /translation="MYVIYINSSLNIKDNLNVPYEEINKGVVDVEEEAVKSITENISS
                     PVEIAALIKSLGVPFNKGFLAPTEYADNYSTNYAQAFALGVFSADLGYLNMYNKTGSV
                     IDYLSTIKKLADAINVGQYFDFATLKRLAANSANIDSLKYISVHSFNQMDQSLRQRKR
                     GNLSSLIIAGAWLEGIYITVQVAKNNTDSRLTDKIGEQKIILADLMTLLISYAKDPIF
                     RNYVSEFNQIKSKFDMVQITFEQGEPITIEENGMLTVIQKDKSIVHVTTEQLNDISKT
                     IEAVRERLIRK"
     gene            18528..19061
                     /locus_tag="PK252_09605"
     CDS             18528..19061
                     /locus_tag="PK252_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15002.1"
                     /translation="MISFLPIPSYIKNINKQVSIISAFLNVRPCCRILILSFFVLFVF
                     SSFETKAQSSSKLAEFCAATAGDDATYLKDFVIELDAAGADGKSPEQMFTLLLSKNSE
                     YRFTVCNAEGSAGKAIFQLHDISKLYVTSYNASTGQTYQSVNFQCQKTGAYHLKVSFL
                     DGKKGSAVVVVSFIRTL"
     gene            complement(19062..20042)
                     /locus_tag="PK252_09610"
     CDS             complement(19062..20042)
                     /locus_tag="PK252_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOT15003.1"
                     /translation="MIPNLHNLFLKISTLLIFLKLSLPGMAQNIEIKPDSLIKLSYKI
                     LARSPESSYGLYGLASGYFMKKDYENALKYSKQNIKKNNQYDTICYYIYSASLVKLGR
                     EAEAIKALKKGLKLFDNYYMLLYENAFLNYKQNKLDEAIGLLSACQKKNPYFAECFYL
                     YAACIYESNNSFSCVYPILYALTLDNQQKRANDAIAFLNLFINKKQSKIEIPFRGSRY
                     KLQSTSDIYSHYTKNDNVEIMQKLQTDIVNQINFLKDEITVNFKNAIDSTISQNFLQQ
                     VSVHNYLDAMLYYCMRSIESQYVQLWFKNNSETLSAYATFLDKTLPGKTQ"
     gene            20130..20882
                     /locus_tag="PK252_09615"
     CDS             20130..20882
                     /locus_tag="PK252_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOT15004.1"
                     /translation="MNNPFMAESVVIIPTYNERENIRKIIEKVFSLEHPFDILVIEDN
                     SPDGTAAIVKQMQMVYPERLYMVERSGKLGLGTAYIAGFKWALARNYQYIFEMDADFS
                     HNPDDLLLLYKACAEDGADLAIGSRYKSGVNVVNWPMGRVIMSYYASAYVRLVTRMNV
                     RDTTAGFKCYRRKVLETIELDKIRFKGYAFQIEMKFTAWKIGFKIVEVPIIFTDRKQG
                     QSKMSGGIFNEAFFGVLKMKVRSWFKKYPLIN"
     gene            21085..22428
                     /locus_tag="PK252_09620"
     CDS             21085..22428
                     /locus_tag="PK252_09620"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT15005.1"
                     /translation="MKTLLIKNATIVNEGRRFQGNIIIEGERIKEIVTTPVDKATGFD
                     EIINAEGKFVIPGVIDDQVHFRQPGLTHKADIYTESRAAVAGGVTSFMEMPNTQPQTT
                     TQQLLQEKFALGAENSFANYSFYIGATNDNLSEIVKTDVKNVCGVKVFMGSSTGNMLV
                     DNPKTLEGIFKDSPLLVAVHCEDEGTIRNNQKKYIDLLGEDLPWHYHPIIRSAEACYR
                     SSSLAVEMAQKHNTRLHLLHLSSAKEMRLLSGAPSTSEKRITSEVCVHHLWFTDEDYE
                     RYGSLIKWNPAIKYAEDRDELWRALLDGRLDVVATDHAPHTIDEKRNIYTKAPSGGPL
                     VQFSLLAMLQFTQQNRLSIEQVVEKMCHAPAKIFQVKERGFLRPGYYADIAIVNPNEK
                     TQVRKEIIFSKCGWSPFEGVLFDYSVSQTIVNGVLVYNNGKINEHFRGKALTFER"
     gene            22482..24191
                     /locus_tag="PK252_09625"
     CDS             22482..24191
                     /locus_tag="PK252_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOT15006.1"
                     /translation="MGSKSDISEKPEEKVSLNFLEEIIEEDIRTGKYQGRVLTRFPPE
                     PNGYLHIGHAKSICLNFGLAKKYNGKCNLRFDDTNPVKEDTEYVDSIKEDVNWLGFQW
                     ANEFYASDYFDQLYEWAKVLIKKGKAYVCDLSAEEISAMRGTPTVPATPSPYRNRPIE
                     ENLDLFKRMKKGEFPDGSKTLRAKIDLASPNMHMRDPVMYRIMHTHHHRTGDKWCIYP
                     MYDYAHGQSDYIEGITNSICTLEFEVHRPLYDWFIDNIFEDPDGYRPRQYEFARLNLS
                     YTVMSKRKLLELVKGGYVKGWDDPRMPTICGLRRRGYTPESIRMFAEKVGVARRDNII
                     DVALLEFCIREDLNKKAMRVMTVLNPIKLVIDNYPAGQTEMLEAVNNPEDESMGNRSI
                     EFSRELFIERDDFMEPPPKKYFRLAPGQEVRLRYAYFVKCTHVEKDVQGNVTVVHCTY
                     DPASKGGNSPDGRKVKGTIHWVNANTAIEAEVRLYDRLFMQPEPEKVEEGHDYKENLN
                     PDSLKVIKAFCEPYLANAKVGVKYQFERIGYFSVDKDSTNDKLVFNRIVTLKDSWAKI
                     SAE"
     gene            24282..24806
                     /locus_tag="PK252_09630"
     CDS             24282..24806
                     /locus_tag="PK252_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT15007.1"
                     /translation="MPAKIRLTRQGRKNIPYYHIVVADSRAPRDGRYIERLGKYFPKT
                     NPASVELNFDRALYWLQTGAEPTDTCRSILSEQGVLLKNHLLNGVKKKALTLEQVETK
                     FSAWLAEKDKKLAAKKDLIKSEVDKAKKQRLEAESKVREAKAAAIAQKAMQKLEAQAE
                     AQAEGEAQAEAEQA"
     gene            24819..25331
                     /gene="rimM"
                     /locus_tag="PK252_09635"
     CDS             24819..25331
                     /gene="rimM"
                     /locus_tag="PK252_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT15008.1"
                     /translation="MIAEPVTFIGKCLRYHGFKGEVVIALEPSVRKYIQKAEWVFINI
                     DNSPVPFFVESISATDQSAILKLKGIDKEKQLPEILQQDVFVASSLFPSKFKPQQNEF
                     QGFTVFSQEKEIGTAHELVDYNGNLLLQVFNHEKEILIPVNDNTIVSVDKRKKKIVVE
                     VPEGLLELYL"
     gene            complement(25326..25931)
                     /locus_tag="PK252_09640"
     CDS             complement(25326..25931)
                     /locus_tag="PK252_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT15009.1"
                     /translation="MPFLPFTHKKTIFVKKEVIKDYYQILEVSKDASIDDIKRAYRRK
                     AREYHPDVSNFRNAAELFILINEAYEYLLAQARLKADKIGANQHEAQEIIDQWLRKER
                     ERIRERARQHASMKYKHFTKTSFYESTDVYSKVIVILALIFGIGIIAGSIIGTIGAIK
                     KEPKLINFSYLGSAIILFTFGVIITLFSALKMNKVVKNKKS"
     gene            26229..27176
                     /locus_tag="PK252_09645"
     CDS             26229..27176
                     /locus_tag="PK252_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOT15010.1"
                     /translation="MKKLLLFSFLFVSFSLSAQINVFSGNNVGIGAGLTSSASTLSVN
                     SAGSSSYAVCVSSTSSYSGGIFCGVQGSNSSPRFGISSSINNLGDGSIKRAIYATAFD
                     ATARTDGQAYGVVGIAGNSTNGYNVGILGQLYGSNNGSGVYGQIGTSFVAPDDKYAGY
                     FYGKVKINGTLWVNSTQITSSDDRLKQDVSSIESVDGLYKLKPKKYKYKSINEQVKAI
                     TGKLSENANDTTVNKEIVDPDPDMAQKTHFGFLAQDLQQVYPELVYQSADGTLGIDYQ
                     GLIPIIIGQMQQMKKSIDDKDEQIKDLKKRVAALEKKKQ"
     gene            27186..27470
                     /locus_tag="PK252_09650"
     CDS             27186..27470
                     /locus_tag="PK252_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15011.1"
                     /translation="MMKALVKKLLTSIVLVNIYIVGHSQAFKSSLEYSYDANGNRVKA
                     SVIYLVKSAKEAVEESTDTIPQIITKNIATDSIPEKGWGLARKIIYRAAK"
     gene            27428..27709
                     /locus_tag="PK252_09655"
     CDS             27428..27709
                     /locus_tag="PK252_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15012.1"
                     /translation="MGPGPEDNISGGKIKLYPNPAHSIIIVEISNSDLNKKEQSNMIQ
                     IFDLQGKMVYHKTGITRYSAIDLSMLSNGTYILKLHLGDQLKEYKIVKN"
     gene            27724..29289
                     /locus_tag="PK252_09660"
     CDS             27724..29289
                     /locus_tag="PK252_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HOT15013.1"
                     /translation="MKKIILTIYLIILVFKTYPQCNNANQVILDGTCAASINAGLVTS
                     NQTVVARDQITMKAGFSVSSVNGKKFEAKIDYTKVYVTNDYLTGTGIVTGTSRPLDKT
                     NCAPGSVGGSINVSPTGAATYNVPIIVSPGTRGMQPNLSIVYNSQSGNGVLGYGWHLN
                     GVSTISRTSKNLYYDGTTSPVKFDANDALSLDGARLIKTGTNQYSPENNPYTVVTTDA
                     SFSFFIVTTQDGVSMEYRQKFLPKGGDIAYAWAITKITDTEGNYMQYIYLGDNTTGEY
                     YISEIKYTGNTGKAPYNSVIFAYDKRTDVNTNYICGKETNKTLLMTSIKVFAEGILAY
                     DYKFSYFYDGYYSKLNQIGLTANGFSYNPMVIDWGASPNFTTTTPTTNSLIISAPYIS
                     DNFAQIVGDMNGDGLQDFAQIVPSDDYPRIEVAFLKSDGSYLFSSVNLKKKVVSGAGD
                     NYEAKDMNVADWDNDGKDEIMVHYLFYKTNSKGDTSNYKHLIYKYSCSGTTINNTPVL
                     DCTLPAGSIKKIV"
BASE COUNT         9400 a         5154 c         5780 g         8985 t
ORIGIN      
        1 acttacagtc cgttagaaca acccaccgcc ttcaggcgtg tggtacatga gtttgggata
       61 agctaatttt ttgcaacaat atattcctaa aaattgagaa ggaaacttta aaggcaaaca
      121 ctgtttaatc tttgcctgta ttagcaacgg acataaaaat caaagcctct ttgaagctta
      181 ttctaatagt tatattgcaa aaacgcgtgc tttaaagatt tgaaaaataa tgattttaat
      241 acattttttt catcatcgtt tcttcattgc cctcgctaac tttgaaaggt tttatgctga
      301 atgagtaccg atattctttt gctggtatca ggtactgcgg atgcaccata gctccccagc
      361 tatcatcgcc tccaactccc atctgggctt tgtctatatg aacagtaatg taccctgttt
      421 ttgaaacatc gcatgcatgg cgtttttcgt atgagtaatt gaggtcattg gtatttatat
      481 tcagtgccga aatggatagg ggctcttttc cgactatcag caatccgttg taaaggttgt
      541 cttttagcgc caaccatctt acatcggtca ggttgccgca ttcctgaggc ctcacatatt
      601 gagtgaaaag gtcttccact gttgtggtgt gcaaggccag cttagcgcca gaattccgat
      661 caatgtaatt ttcatgaggc ccacgtccgt acatagttat atagctgtat ttagcgggta
      721 tgcccatctt taggcctacc ctgggcatgt ctggcaaatc tttcccggga acaaaacggt
      781 aatcaaaaat aacctgtcca ttgccaaaaa tgatatactt gatgtacaac ttgcttttta
      841 tttcgggcaa agtaaaaatg gtgtttacta ttacctgcca ggcattcacc ggggtaagga
      901 caaatgagtc aactttggcg ttttgtgtag cttttttcca tatagcaagc ctttccggca
      961 ttttattccc aaagtcattg tctgtcgggg cccgccaaaa gtctgcctgt ggggccatct
     1021 ttatcatttc tgttgtgtca aatttgtatg ataccaggct tgctgtctgt gcatcaaata
     1081 caatgttgaa atttggcccc gatatggatg gcatgccttt ggcgttggtc ttaacattga
     1141 gctttggcaa ttggtttaca tcaaaaggtg tgcgggcttt tgaaaaaggc aggataaact
     1201 gatcccatgc cacttcaaag tttttaggca cagcagcagt ggcaactttg gttttatagc
     1261 tgaaaataat gctatactct gccccctctt tacgttcaaa aagaggataa gcaacagtta
     1321 tttctttgct tttgtgtggc actaactgtt gtacgccaag gttagcaccg gctataggtt
     1381 ttccgttttc taaaatattc caactaatgt catatttact catttcggta aagtccattt
     1441 cgtttacaat ggagaatttt cctgcactca aatctatggc tttgacgtgc agtgattgat
     1501 attgcttttt cacttcccag aggccgggat gaggcttgcg gtttgaacca accaaaccat
     1561 tacagcagaa atttgagtcg cttttgatgt taaaaagcat cccaaagtcg cctccataca
     1621 agaaacacgt atctttgcca tttactgaag caaacgattg gtcaacccag tcccaaatgc
     1681 atcctccctg aaggtgtggc gtggcgcgta ttacgtccca gtaatctttt aaattaccat
     1741 tgctattacc cattgcatgc gaatattcac acataatcaa tggcctgtcc tgtttttttg
     1801 agccatattc tttcaggtgt tcgatctttg ggtacattgg acaatatatg tctgtgtttt
     1861 tacttagtcc ggcctgttcg tattgtaccg gacgggtctt gtcccgttct ttgagccatg
     1921 cataagtggc ttcaaagttg ctgccattgc cagcttcatt tcccagtgac caggtaataa
     1981 tacacgggtg gtttttatct ctttcaaaca tcctctttgt tcggtctata tgggctgcaa
     2041 gccattgctt gtttttggca agggtttgtt cgtcccactg gtgccaaccg tggctttcga
     2101 tgttggcctc atctataacg tagagcccgt attcgtcgca aaggtcgtac cacaatggat
     2161 cgttcggata gtggcttgtt cggacagtgt ttatgttgaa ttgcttcatc aggctgatgt
     2221 ccctgcgcat ggattcttct gatattacat ggcctttgac catgtcatgc tcgtggcggt
     2281 ttacgccttt gattagcacc ctgactccat ttaccagcaa gtccccatct tttatttcta
     2341 tcttcctgaa ccctgttctt tgggtaatgg cctcaagtac attgcctttt ttgtctttca
     2401 ggactacaag aacggtatat aaatcggggg tttcggcagt ccatgttttt acattcacta
     2461 ttttttcttt ccactcaact atagcagtat cattgatgtt tactttaaag gttttcggag
     2521 aagctgcatt tttaccttca ttattaatga gtgtagcttc tatttggtaa tctttatcag
     2581 gttttccggt atagtttttt ataaaaatcc ttgatttgaa aagtccattt ttatatgagt
     2641 cgtctaaatt actaataatt tcaatgtcac tcaattgtac ttttggagta gaataaagac
     2701 aaacatcacg ttcaagtccg cttagcctaa aaaagtcttg atcttcaagg taactgccat
     2761 cgcaccatct gaaaacttgc acggcaatgg tgttctctgt accagcttgt atgtatttac
     2821 taatcctgaa ttcggctgga gttttgctat ctttgctcat gccaacgtat ttgccgttta
     2881 cccacagata tatcatggag tttgctgcac ctgcctggaa aaatacttct cgttctttcc
     2941 aacctgccgg aatgtttatc tttttgatat acgaacctac cgggttgtaa tcagagggaa
     3001 ccattggagg gttagcaggg aatgggtacc tgatatttac gtaaaccgga atgtcgtatc
     3061 cttgaaattg ccagtctgat ggcacttcaa tgtctttcca gtctgaactg ttgaatgtgg
     3121 gctgaaaaaa gtcttttggc ctggagtctg ggttattggc taggttgaat ttccattttc
     3181 cgttcaatga tatgtaaaac ggtgaatttt tgatgtttct ggtcaatgct gatttagcat
     3241 cagcgtatgg ataaaacaac acgtgaggag tctctgcatt gatgctcacc aattctgggg
     3301 tttcccattc gttttgggcg tagttgctta tgcaaatgca gcttaacgct gtgataattg
     3361 taagtgttag ttttttcaac atatttaggg cgtattaggt ttaaatgtca gaaataacgt
     3421 atgcttcatt gaaaatgatg aagcatacgt ttttcaaaaa caaagttaaa aaaaagtaga
     3481 ttaacctttt attttttaga tatttctctt acaaaggaaa tgtatagtat agcgaatagg
     3541 tgaagccaaa gttgtatttc tcagcacctt ttccaatgcc ggggatgtag taaggcttta
     3601 ccgatgaggt attttgctgg tcgataatat aggaaaaccg gacagtccag ccagcggcaa
     3661 aatatttgaa gaattgaact ttgatacccg aggtcagttc caaccaatgc gtttgtatct
     3721 cttgtgttga caatgatgta tatcttttag gccagaattt atcgtttatt ataatattct
     3781 ttgcactatg attttggttt gcataaccgt atctaaatcc aaaaaatacc atgttgtttt
     3841 ctccaaagag gtttcttctg aatgtgttgt aatcgaagcc aattctgaag aacttgccat
     3901 ccgattgata ttcatatgtt ttaatcgtat cgctttggct taattcaaca ttctccaatc
     3961 caaactcaac aatagggttg aagttgttaa tgttgctata tgatattgaa ccttctactt
     4021 caaacttgtt tttgtctatc atgtaaataa caggtttggc aatgtcaatg cctatgctta
     4081 gccttttttg tacagtgtct ttttttacaa tctgcgcaaa accgatgttt gccaatgaaa
     4141 atattgacat aaacacaaca gaaacagata ttttagaaaa gtatcttgtc attttttctt
     4201 aaaatcagtt ctttttcgga tgactatttt gtagtttacc ccgtattcaa tgtctttatc
     4261 gacatagctg tctataaaaa gtaccgaagt tatgttattc tttgagtatt ttagatgttc
     4321 gaggttaaat ctcgtggtaa aaccacattc gggcgaaata agctctaaat ttcttttata
     4381 gaaaaaggtt aacgtatctg ttgcaatact atcaaaggct atgtaaaatg ttgaggtgtc
     4441 tgcaacctgt gataagggca atccaatctg ctgccctgaa gctgtatcgt acaacggtaa
     4501 gctgtcagaa gtgacaatgc tgaatttact tattgtagta tcttttatcg tctttccatt
     4561 ggctgttaca aatgttaacc ggactttcct gttgcttaca taaaaacagt cagaagaatc
     4621 tgtacatgaa aaaaacgata acaataaaaa tatagtcagt ataaaatatt tcagaggata
     4681 ttgcatttgt atggtaatgc cgtaaaactt ataaataatt gtttttgcca caaattcaat
     4741 gttttgttgc gtcttttttt tattttcccg tgggcaaata tagcgcatat aaagtttttc
     4801 aaaacctaaa ttgtgataca aacttaaaat taccagcatg ttaaatttta aaattatgat
     4861 tatttattgg ggcccaatat tcttctcttc gccaaaagtg ttatttttat atttattaca
     4921 aaaacagtgg gcataattta tttactattg ttagatttgt taattttatg taagattccc
     4981 ttgaatgaca attgaagata tagacataca atattttgtt acagccctta aaaattcttc
     5041 gggctacgat tttagcgagt attctgaaaa gtcgctaaaa cgtcgtttgg ccaaagtgtt
     5101 aatggataat aaaatggagc ttaaccagct cgttactgaa atcaaaaaaa atgtttcatt
     5161 tgccgaaaag gttgttaagg agataactgt caacactacc gagctctttc gcgacccgtt
     5221 catttggcaa atgcttcgtt ataaggtatt gcctacttta caagacaaca aaattatcaa
     5281 catttggcat gccggatgtt ctactggtca ggaagtatat tctatgatga tccttttaaa
     5341 tgagcttggc atgctcgaaa aagcccgcat ttttggcaca gatatcaatt ctgatgtact
     5401 tgacattgcc cgcaaaggca tatacaaata taggttcaat atcggttatc tcgataattt
     5461 tgataaggtt attaaagaaa atccttataa ttacgaaaaa ttcaatgata taccttacag
     5521 caaatatttt gaaattgata aagtaaagga ttttatgcgt atgcacccct ttctgaccga
     5581 gaaacctact tacaaaaagc acgatttggt gagggatgaa aatatttttt tcaataaatt
     5641 cgatatcatc ctttgcagga atgtaattat ttatttcaat tacaaccttc aaaaccgcgt
     5701 gtttaacttg ttttcaaata gcctgtacca gggaggcttc ttattgcttg gtatgcacga
     5761 aactattctg gggccaatgt ctgcatcttt tgataaaaag gaaaacatat atattaaaaa
     5821 atagtcatat ctatctttaa ttataaaaat gagatgaaac ctgtcatact tacactgaaa
     5881 aagaaaatag cagccatctt gttattgtta ctaatacttg tgcttgtatt gttttttacc
     5941 gctttaaacc agtttaaaat tgtaagggcc cacacaagcc tttatcaaag ggccaataat
     6001 ctggtttatg ccagacagca cattgatagt ttacaaaatc aggttcagct tgctttgccc
     6061 gttaatttga atttcttcaa aacaggccaa tctaagctca ttgatgagat taaagattac
     6121 aacatcaaga ctattgaaga aattaacaaa atatccaatg acttttactt aaaaaatact
     6181 aagtctgtca accttaaagc tttttatatt tcacaaatcc ttagaaatta caacgaaaca
     6241 ttggacaaat ataccaggct cctttacgaa aaaggctttg agcaatatgg acttgtggga
     6301 cagcttaaaa tccttactga ttatttaatg gttaattttc agcaagaaaa taatactgcc
     6361 ttaattgcta agctcaaaga aataacccag ctgaaagata aatacctgaa agaaaaagac
     6421 cctgtcatta ttagtaaaat tagcatcatt actgacgaag ccaaagtaca ggctatcgtt
     6481 tctgtaaaaa aagacaaagc tactgtgctt ctcaagcttc aaaagtttga agagcttata
     6541 aaacaaataa accagataga cgaaactatc gggtattctc ctactgaagg agtatccggg
     6601 accctaaaaa ttattgccga caattatcat aaccaggctt ttttatttaa aaaaatcatc
     6661 gaacgtaaaa ttgacaatgc cgtaaaatgg gcctacacat ggattattgc tatttttgtc
     6721 ttgttagtat tgaccctggc cttgttgttt gatacaataa acaaacaaat acacaaacct
     6781 ctgcgaaaac tcaccaattt cttagaacag cttgtaaaag gaaagttgcc ggaaaaaata
     6841 cgtcttaaag gaaatgacga gatagccgtg atggcccgaa acttaaatga tctcaccgat
     6901 ggtttaaggc tgaaagccga gtttgctatg gaaataggga agcgaaacca aaatacagtt
     6961 tttcaacccc ttagcgatga cgacatttta ggcaatgccc ttatcgagat ggaaaaaagc
     7021 cttcgcaaag ccgatcttga agaccaaaaa tataaaaccg aagagaaaaa acgcatttgg
     7081 acaaatgaag ggttggcccg ttttggcgaa atcttacgtt cgcacaataa tgatattaac
     7141 gtgttagccg atgaggttat tcaaaacctc gtcaaatacc ttaatgcaat acaaggagct
     7201 ctttattttt acaatgatga cgaccctcaa aacatccatc ttgatattgc cgccgctttt
     7261 gcttatgacc gcaaaaaatt tctaaaaact tctatcactt tgggcgaagg ccttgtaggt
     7321 actgttgctg ttgaaaaaga aaaaatattt attacagatg tccctgaaga ctattttaca
     7381 ataaaatcag gacttggaga ggccccaccc cgcagcatac ttattgtacc tcttaagctc
     7441 gaagatgaaa tgcttggtgt tgttgaattg gcttcataca atattttcca acctcacgaa
     7501 attgaatttg ttgaaaaaat tggtatgacc attgcttcca ctattaccaa tgtaaaaata
     7561 aacgcacgta cagcccaact gcttgaacaa tcgcaaaaac aagcagaaga tatggctgaa
     7621 caggaagaag agatgcgcca gaatatggaa gagctccgca cgacccagga ggattttgcc
     7681 cggcgcgaag ccgaattaca aggtttgttt acggccgtaa aagacaatgc ccttttgtta
     7741 accatagata aagacggagt tattattgag gtaaataata atcttataaa tactttacgt
     7801 tctaagcctg aagatattat aggccgcaac tatcgcgatt tttctgtatt ggctaaaaat
     7861 ggacacgaat acgatgtctt tttcaacaat ctgtttaatg gcgaaagtca ggtaattgtt
     7921 gagaaaatac gtctctctaa tgccaaagat ttatatcttg aacaacattt tacccctatt
     7981 gctgatttgc atggcgttta taccaaagcc atttgctttt cctataacat tactgagcag
     8041 aaaatacttg aaaccaagct cgaactacag aaaaaagaaa ttgaggccag cgaacaaaaa
     8101 cttaaagatg ttttcaatat cattgatact tcattgttga gatgcgaata caatggtgat
     8161 tgtaaaatta ttgatgtcaa cgaaaattat tgcagacaaa caggccaaac caaaagtgag
     8221 tacattgcta agcccggtag tatttatctc aaggacgatg agaaagacca atttgataaa
     8281 atctggaacg aagtcttgaa aaataaaaat tatgttggag ctgttaaaag gaccaagcct
     8341 acaggcgaag agttctggct tatggctgct tttacgccag tttgtgatgc ttccgggaaa
     8401 ctgttaaagg ttagcatgtt tgcccttgat attaccgaga aaaaacttaa atatcagctt
     8461 cttgaagagg caaataaaga aattgaacgt ttgaaaaaag catctaatga caaatgacaa
     8521 ttcatattct ttgaccagtc caattgctgc agaaattggg attgtcgaat atcgtagtgt
     8581 tatcagggct atttctgaga catacgggta tgatttttct gaatttgccc ttacttcaat
     8641 gaaaagaaga attgaaaact tcatttctgc ctttaatctg cggagcgtag aagcccttat
     8701 cgacaggctg aaaaatgaca aacagtattt tcaaacattc ttaaaatcaa tttcggtaga
     8761 atctaccgaa atgtttagag acccttcttt ttggcgaacc cttcgggatc agcttttgcc
     8821 ccttatatta tctaaagtgg caaggccccg ggtctggttc ccaacatgcg tttcgggcga
     8881 tgaactatac acattctgta ttgttttgtc tgaaagtaat tggctcgaca aatttgaaat
     8941 aatagccaca tactcaaatg atttgagcct caaacaaatt caaagcggac agtttgacgg
     9001 gcataaatac gatgtttctt ccgataatta tgagcgctac cagggtaacg gaaaaataag
     9061 ggaccatttc atttctgccg aaatcaaatt tcaaagaaat acttcattaa ttaaaaatgt
     9121 acattttatc aaacaaaatg ttaattttga taattcacca acagatgtaa agttgattat
     9181 attcagaaac caaatgctat acttcactca gccccttcaa gacaaggtgc ttacaaagtt
     9241 atacaatagc cttggacaag gagggtttat tgctattggc gttaaagagc aaatggggat
     9301 gattagcagt aagtatttca gggccattaa tgaaagtgaa aatattttta aaaaaatata
     9361 aacaatatta aaaaagtgta taaagccgta atgataggtg gttcagcagg tagttttcag
     9421 gtagtggctt ccattttagg aagcttgcct aaaaattacg aattacctat ttttctgtgt
     9481 ttacatcgct taaagcatgt caggacaggg tttgttgaag tattgtcttt gaaatctgcc
     9541 attcctgtta ttgagcctta tgataaagag cccattaaac caggaagagc ctatttagcc
     9601 ccggccaatt atcatatgta tatcgaactg ggaaataagt ttgcactttc tacagaagcc
     9661 cccctgaacc actctcgtcc ctccatcgat ctatcatttg ttactgcagc acaagtatat
     9721 cgaaacaaat tggtagggat aattctttcc ggcgcaaaca aagatggagc ttatggattg
     9781 aagaaagtag ccgaatatgg cggtaccaca atagtacaag accctaacac atgcgaagtt
     9841 agaaccatga cagaggctgc attacaaatg ggaaacgtaa accatatctt ttctattgat
     9901 aaaattgctt catttgttgc caatctaaaa gctatatgaa aactttaaac aaatataaaa
     9961 ttgcttcttt cgtcatatta cttctggtga taatagtttc tttcctaatc acttaccaat
    10021 ttctaaattt aatcattgcc caaaagattg cttcggcatc catattatac gttctgttaa
    10081 ttttgggctt aaactttagt atgtttttca taatatttaa acttgtgcag gttattattg
    10141 aagaagaaac tactttgcaa aaaatactcc agcagaatga agtgcttgtt gaaaatgctt
    10201 caactgaaga caaaaaagaa gaatataaag atgtaaatat tgatgagctg gtacagaaaa
    10261 ttatcccggc tattcctcaa aatcaatctt tagatgagtt ttgcggcaac cttttaacaa
    10321 acctctcaaa gcagttaaat atagtacaag gcattgttta ttgtaaaaat aaccagacca
    10381 gccagtttga gctcaaatct acatatgcac tttataccac tgagccaata aacccttttt
    10441 ttgaaggtga aacactccca gggcaggtag ctgctgataa aaaagcaatt tgcattactg
    10501 atgtccctga caattatttc agaatagcat caggtttagg ctcagctaag cctaaacatg
    10561 ttgtcatatt tccaattatt gacaaagagc ataccattgc cgttgttgag attgcgacat
    10621 tccaaaaaat tgaagataat acatttgaga tttttaataa actatctgca attttgggta
    10681 aaattttagt taaacttaaa tagtttttct atagatggct aatactataa aatataaaac
    10741 atgggatcct gagatatttt ataaatcttt ggttgcaggt gtggcatttg taattctacc
    10801 tgtttcattg tggattgtag aacttgccct ttggcaagtg ccgctttcat tagatagtat
    10861 aatttttata catcaaaaaa gtatttcatt atgggtggcc gatttaatgc cttttacagt
    10921 gggggtatta gtttatcaaa ttattatccg ccggaaaaaa tttgcagaca aacttcaaac
    10981 taagattgaa gaaatgcaac ggcagatgga tattaacgcc aaatttgcca aagaaattgg
    11041 agaaggaaac tataacagcg aatttaagct ttctgaaagt aacgatatat tagccacttc
    11101 attgttgttg atgcgcaata accttcttaa aaacaacaaa aaagaagcag agcaaaactg
    11161 gattgctgaa gggaaagacg aaatatcaaa tatccttcgt ttacacaaca aaataaatga
    11221 cctggcttat gaagtgctta taaaacttat taattatata gacgccatcc agggtgcatt
    11281 ttatgtttac gatgacgaaa aagacaaaat agtaaatgtt gccacatatg catacaaccg
    11341 caagaaatac cttaaccagg aattttcttt tggcgaaggg cttgttggcg aatgtgcttt
    11401 tgagatggac attatctatc gcacagaaat ccctgacgac tatttttcaa tctcaagcgg
    11461 ccttttagga gatagaaaac ccaaaagcct gcttattgtc ccgcttatct ctgatgaaaa
    11521 attgcaggga gtacttgaat ttgcttcatt aaaagacgaa atccctgaac tcaccattac
    11581 ttttatgaaa gaacttggcg agatcattgc ccgcacaata tttaaccttc gcattaacca
    11641 aaaaaccgaa cgtttgcttg ccgaatcaca gcaaatgacc gaagaactcc aggaaaacga
    11701 agaacaactt cgtcagaatg ctgaagaaat gagggcaacg caggaagagt tgcagaaatc
    11761 taatgaaaag ttggaaaatc agataaaaga agtagaaaat gcccaaaaac gcatcaactc
    11821 attgttagag aactcctctg agattatcat gatttatgat gaacatctga accttactta
    11881 tcaaagtcct tctgtacgaa aaattttagg ttacttacct gaagaaatga cccacgggaa
    11941 atattttgac cgtcttaccc gcaaaggcga gcaggaagtt cggaacatgt ttaggcaatt
    12001 gctcgaaaac ccccttgaag ttaccacaat acaatatacg tacataaaaa aggacgggca
    12061 aaagattttt attgaatctt caggccgtaa ccttttgaat gaccctgcaa taaaagggat
    12121 actgctcaat tctcaggaca ttaccgaacg taaacgtgcc gaaaaagaag agcgcatgaa
    12181 gagtaagatg cagtctcttt ctgaaaattc gctcgatctt attttacgcc tcagtttgca
    12241 aggccacttc ttttatgcca accccattgt tgaagattat tttggccttg agcccaaagc
    12301 agtaatcaac aaaacagttg atgaaatacc tatggatgag gcccttaatg aatactttaa
    12361 agttaccctc aaacagttga ggacaaagcc tgaaaaacgt aaccaggaaa ttaaagtacc
    12421 aattaaactt ggcgaaaaaa taatttatcg aattctcaat attgatgcca ttccggaata
    12481 taacgaaaat gaacttgaaa cagtcctttt tgtagggcac gatataacag aagcaaagcg
    12541 tattgaactg gaaattcagg aaaaaaacaa aaaaattgaa gactccatca attatgcaca
    12601 acgtatccag tcgtctattc tgccagataa caggcttata cgtgaattct ttcccaaatc
    12661 attcatttat tataagccac gagatgtagt tagtggagat ttcccctggt ttttccatca
    12721 tggcgatgac atttatatag ctgccgttga ctgtactggg catggtgtcc cgggagccat
    12781 gctttcattc attgggtatt ttctgttaaa taatattgta gataatgata aggctttaaa
    12841 tgccggttct atttgcgaca tgcttcatga aggggtccgt acaaccctga aacaagacag
    12901 ggaagatgcc agtgggcgcg atggtatgga cattgctata tgtaagataa atgttaagaa
    12961 aaaagaactt cattttgccg gagcacatag gcctttatac ttggtgcgta aaggagaatt
    13021 gattgaatat aaaggcgacc gtaaagctat tggaggtatt cctttaggga ataaggccga
    13081 acaaccattt actaattacg taatcaatat tctcaatggc gacagggtct tctttttctc
    13141 tgacgggctt cccgatcaac ttggcggccc cgaacataag aaatactcgc cggggcggat
    13201 aagaaacctc atctgcgaaa ataacagttt ttctatgtcg caatattatg agctttttgt
    13261 tcaggatttt gaagaatgga agggcgaaag caaacaaata gatgatgtgc tacttattgg
    13321 tattgaattt taacagtacc atcatatgaa tatttttact aaatttgatt tttgaaggta
    13381 taaaagaaaa atgcaatgga gtcaataaaa aaagattcaa aaggctttct cgaattcgtt
    13441 tacgaatttt ataaaacgat gaaagcacac gaaatatctc ttgtttatga gggcgaaatt
    13501 acccatcaga ttaccaaagc ttttacatct ttggcagaaa gtaacatggc taaagaagaa
    13561 gaatcaggta ctgtgcaacg taaagtattt cacgtcatgg tagaatgttt gcaaaatata
    13621 agcaaacatg ccgatgattt tgcagataac gactttttat attcaggcag gggcattttt
    13681 atggtcagca aaggagaaac cgaatacaca gtaactaccg gtaatgccat agacaattct
    13741 aagatgaaag atatgcaaga aatgcttgat cgcataaaca gcatggataa agaagagtta
    13801 aacgaacttt ataaaaaaca aatcaaagaa ggcagattgt cagataaagg tggtgccggg
    13861 cttgggttta tagatattaa aagaaaaaca ggtaaaccat tagaatatca cttcttgcct
    13921 attagtgaaa atatatcgtt ctttttactt acttcttcaa tctcacgtga ataattaaaa
    13981 tattacaata tggaagccat aaaaataaaa ggaactgaag atacccctag tgttattttg
    14041 gatgctgctg aaaatattat ggaaatatca gggcgttcat tgccggagga tgttagtagt
    14101 ttctatgggc caatattatc atggcttaac gaatatgcca aaagcccaaa ccctaaaaca
    14161 gtttttgttt ttaaattagt ttatttcaat actgcatctt caaagcttat tcttgatatt
    14221 ttgatgaaac ttgaagaact tgccaaaaat ggtaacgagg ttttggtcaa atggttatat
    14281 cctgaagatg atgaagatat gcaggaagca ggtagtgaat atgccgatat tgttgaagtt
    14341 ccttttgaac agatagctta ttcctagttc atagaccata tcctttcggg attggcaaat
    14401 ttttaattta tgagtgaaga gattctcaaa gcgctaatgc aattatttgc aataattgca
    14461 aaacaagacg agggtgttga aaatacagag aaagagtatg tacgtaactt tcttagccag
    14521 caattgtccg acgagcttag taaggaatac tatgctttgt tcgaaaaaca tgctgaatgg
    14581 ggcgatgaag aagaaaatac tgaaggggaa aagaaaaaac gccttaccaa ggttaatgac
    14641 tctgttcgta ttttaggtat ttgccgaaag attaacaaaa cacttactca aaaccagaag
    14701 atagtagcct tagtccgtct ttttgaattg gtcaatgctg acagaaaatt ttctgaacaa
    14761 cgcatggcta ttatcaacac cgtttcagag gtttttaacg ttggcgttga agagtttaaa
    14821 agtattgaaa aatttgttat acaaaatgag cccgaaaata ttgacgatcc gcttgtttta
    14881 gttatcaata gtaaagaaaa acatgattat gcttgcaaac atttgcatgc cgataacctg
    14941 gataaaaata ttttcatcct tcatattccc agtgtagagt tatattttct taaatatacc
    15001 ggagagcagg atttgtttct taacgggctg gccataaata acaaacgcat atatctgttt
    15061 gccagcggaa gctcggttaa attgcccaaa gcaaaaccta tatactattc ggatgttgca
    15121 gcacattttt tggcagatat ccatgccagc aagatatctt ttgaggtcaa agaccttgaa
    15181 tataaatttt cgaatggcaa tataggttta aggaacatta atttttgcga gagccacggt
    15241 cgtttagtag gtattatggg agccagtgga gcgggtaaaa ccaccttgct caatgttctt
    15301 tgtgggattg aaaaaccatc ttccggcgaa gtcctcatta atggtgttaa ccttcatact
    15361 caaaaaaaag aaatagaagg cgttataggc cttataccac aggatgacct tttgattgaa
    15421 gaccttactg tatttgaaaa cctttatttc agcgctaagc tttgttttaa agacaagtct
    15481 gaagaggagc ttgtgcaaat ggttgataat actttattta accttgggct gcttgaccga
    15541 aaagatttga gagttggtaa tgcactaaat aaaactattt cgggtggaca gcgcaagcgt
    15601 ttgaacatag ctcttgagct aatccgcgag ccgtctattc tttttgttga tgaacctact
    15661 tcggggcttt cttcaagaga ttcggagaat gttatggacc ttcttcggga acttgcgcta
    15721 aaaggcaaac ttatttttgt agttattcac cagccttctt ctgagatata caaaatgttt
    15781 gataaaatga ttatccttga caccggaggg tatcagattt atagcggaaa tccggttgag
    15841 ggggtgatgt atttcaaaaa aatagatgca cagattaata gtgaagtagg agagtgccct
    15901 gtctgcggta acgtaaaccc ggaattgatt ttcaatatag ttgaagcgca ggttgtagat
    15961 gagtttggta attatacggg ggtacgcaaa gttgcaccac acaaatggga agagcactac
    16021 cttaacaatt ttgccaaaga aaaggtagat accattaatg atgcccctcc aaaatcattg
    16081 aatatcccta attggctaaa tcagttaaaa atatacatca aacgagattt tcttgctaaa
    16141 gttagcaata aacaatacat catcctcaat ttgcttgagt atccggtttt agcattaatg
    16201 ctttcatata taatcagata cattgccgat cctacatcaa gcgtttatat tttccgtgaa
    16261 aacgaaaatg tttcgatata catatttatg agtattattg tggcattgtt ttttggcctg
    16321 actgtcagtg ctgaagaaat attccacgat cagaaaatat tgaaacggga aaaattcctg
    16381 aatttaagca aatcgagcta tttattatca aaagtcctta ttttgtttgc tttatcagct
    16441 attcagacat ttacatttgt actaataggc aattcaatac ttggaataaa ggccatgtat
    16501 tttacctatt ggttagctct tttttctatt gctgcctttg ccaatatttt agggctcaat
    16561 atttcagcat cattcaattc ggtggttact atttatatta tcatcccatt tttgatgata
    16621 cccatgatgg ttttaagtgg ggctatgttt agttttgata aactaaaccg gacagtaagc
    16681 agtgttgata aagtacctct tgtggcagag gtaatgccta ctaagtgggc atacgaagcc
    16741 cttatggtac accagtttaa agacaacctt tatgagcgaa atttttatga atatgaaaaa
    16801 gaaatcagta atgccaactt taaactcgtg tattatatcc ctgagctcat caaaaggtta
    16861 gataaagtta tgacagagct caatacttcg cctaaagtag agaaaacaaa agatgatttg
    16921 cttcttcttc ataatgagct tttggcccaa tgccaacaag tcccttttgt agaatgcaaa
    16981 atagccaatt gtctcaacgt agatagtttt gatatgcagg cttctatcaa tacgatggaa
    17041 tacattaatg cgttaaatga ttattatcag aaggtttacg atgctgcaga caagagaaaa
    17101 aacaacatgt tgcaatattt agatgaacat aagcctcagg tttacaaata ttattatgac
    17161 aatttttaca atgaccattt gacagatatg gtaaagaaag cttttgagaa ggaaaaaaac
    17221 aaacttcttg aatatgacca taggttgatt caacaaacag accctatatt tttagacccg
    17281 agagtgtctc ataagtttga tttcagagct catttatatg ccccacggaa acattttctt
    17341 ggcaaatatt atgataccta ttggttcaac atatttgtca tctggtgttt ttctgtaatt
    17401 ttttatttga gcctttattt tgatttgtta aaacggctta ttgattttcc tgataaggtt
    17461 cgtttgcatt atcggaataa gaaagttgaa taacagttta tattattatt atggtgcgaa
    17521 aaattagttt catagcaatt ggggtattac tcttaagcgt ttgcatgtat gtaatataca
    17581 tcaacagcag ccttaatata aaagacaatt taaatgtccc ttatgaagaa ataaataagg
    17641 gagttgtaga tgttgaagaa gaggctgtta agagtattac agaaaatatt tcatcaccgg
    17701 ttgaaatagc agctttgata aaaagtttag gggtaccttt taataaaggt tttttggccc
    17761 ctacagaata tgctgacaat tacagcacaa attatgcaca agcttttgcc cttggtgttt
    17821 ttagcgcaga tttagggtat ttgaacatgt acaacaaaac aggttcagtg atagattacc
    17881 tttctaccat caaaaaactg gcagatgcga ttaatgtggg gcaatatttt gatttcgcca
    17941 cactaaaaag gttagcagcc aatagcgcta acattgattc attgaaatat atttctgtac
    18001 atagttttaa ccagatggac caatcgctga ggcagcgaaa aaggggcaac cttagttcgc
    18061 tcattattgc cggtgcatgg ctcgaaggga tttatattac agtacaagtg gcaaaaaata
    18121 atacagattc tcgtttgaca gataaaattg gcgagcaaaa aattattctt gcagatttaa
    18181 tgacattact tatttcttat gccaaagacc ctatttttag gaattatgta agcgagttta
    18241 atcaaataaa aagcaagttt gatatggtac agattacttt tgagcaggga gagccaatta
    18301 ccattgaaga aaatggcatg ctgactgtca ttcaaaagga caagagcatt gtacatgtaa
    18361 caacagagca gttaaacgat atttctaaaa ctattgaagc tgtcagagaa aggctaatca
    18421 gaaaataatg ccatgtgtta ttttctattt agatagtttt gtatttttgt tacgatataa
    18481 agcataaaca aaatcatact tttgaaaaat tataattgca atctgaaatg ataagttttc
    18541 tcccaatacc atcatatatc aaaaacatca acaaacaagt ttcgataatt tctgcatttt
    18601 tgaatgtaag gccatgttgt cgtattttga tattaagttt ttttgtactt tttgtatttt
    18661 cttcatttga aactaaagca cagtcttctt ctaagttagc agagttttgt gcagctacgg
    18721 caggagatga tgcaacatat cttaaagatt ttgttataga gcttgatgct gccggggctg
    18781 atggcaagtc gcctgaacaa atgtttacct tattgcttag taaaaacagt gaatatcggt
    18841 ttactgtttg taacgcagaa ggatcggcag gtaaagctat ttttcaatta catgacatct
    18901 cgaagttata tgtaacatct tacaatgctt ctacaggcca gacctatcaa tcggttaatt
    18961 ttcagtgcca gaagaccggg gcatatcacc tgaaagtatc atttttagat ggcaaaaaag
    19021 ggtcggccgt ggtagtggtt tcttttattc gaacgttgta actattgagt tttgccaggc
    19081 aatgttttat ctaaaaaagt tgcgtaagca gaaagggttt cagaattgtt cttgaaccaa
    19141 agttggacat actggctttc aatacttctc atacaatagt aaagcatagc atctaaataa
    19201 ttgtggacag aaacctgctg taaaaagttt tgactaatag tagagtcaat agcgtttttg
    19261 aaattaaccg taatttcatc ttttagaaaa tttatttggt tgacaatgtc tgtttgtaac
    19321 ttttgcatta tttcaacatt gtcattttta gtataatgtg aataaatatc agaagttgat
    19381 tgaagtttat acctgctgcc cctgaaaggg atttctattt tactttgttt tttattgata
    19441 aataagttta aaaaagcaat agcatcatta gcccgctttt gttggttatc cagcgtcaat
    19501 gcatataata ttgggtaaac acaagaaaaa ctgttgttgc tttcataaat acaagcggcg
    19561 tataaataaa aacattcggc aaagtaaggg ttcttttttt ggcatgcaga taacaatcca
    19621 atggcctcat caagtttatt ttgcttataa tttaaaaaag cattttcgta caatagcata
    19681 tagtagttgt caaatagctt taaacctttt ttcagggctt ttattgcttc agcttctctg
    19741 cccaatttga ctaatgaggc tgaatatatg taataacata ttgtatcata ttgattattt
    19801 ttttttatat tctgtttgct atatttaagt gcattttcat aatctttttt cataaaatag
    19861 cccgatgcca aaccgtataa accgtaagat gattcggggc tgcgggcaag gattttgtag
    19921 cttagcttaa tcaggctatc cggctttatt tctatatttt gagccatacc aggcagagag
    19981 agctttagaa atataagtaa agtagaaatt tttaggaata gattgtgtaa attagggatc
    20041 atatcaacaa agtttgattt gcgcaaaagt accttaataa ctttatttct ttcgggtttt
    20101 gaatttaata tttatcttcg catcagtaaa tgaacaatcc ttttatggca gaaagcgtag
    20161 ttataatccc aacatacaac gaacgtgaaa atatccggaa aattattgaa aaggtttttt
    20221 ctttagaaca cccttttgat attttagtta tcgaagataa ttcacctgat gggacagccg
    20281 ccattgttaa gcaaatgcag atggtttatc ccgaaaggct ttatatggtt gagcgtagtg
    20341 ggaagttagg gcttgggact gcatacattg ccgggtttaa atgggcgctt gcccgcaatt
    20401 accagtatat ttttgagatg gatgctgatt tttcgcacaa tccagacgat ttgctgttgc
    20461 tttataaagc ttgtgctgaa gatggtgctg atttggcaat tggttcaagg tataaatcgg
    20521 gggttaacgt tgtcaattgg cctatggggc gtgtaatcat gtcgtattat gcttcagctt
    20581 atgtaaggct tgttacgcga atgaatgtaa gagatacaac tgccggattc aaatgttaca
    20641 ggcgaaaagt acttgaaact attgagcttg acaaaatcag gttcaaaggt tatgcttttc
    20701 agatagaaat gaagtttacg gcctggaaga ttggctttaa aattgtagaa gtccccatta
    20761 tttttaccga caggaaacaa ggccagtcga aaatgagcgg agggattttt aatgaagctt
    20821 tttttggggt tttaaaaatg aaagtgcgta gttggttcaa aaagtacccg cttattaatt
    20881 gattttaaga tataaggaag ttaaaacgag catgtatgca actatgcgga gcgccctgat
    20941 ggacgaaata aataaggcag attaaaaaaa ttaacatgcg agttcttcgg cccaaaacag
    21001 gataagctat catagcacac attaaatagc atttaatgat atggcttaaa tggctaaatt
    21061 ttaacaaatt aaatttttgt ttggatgaaa acactgctta taaaaaatgc gacaatagtc
    21121 aacgaaggcc ggcgttttca gggtaatatc attatagaag gggaacggat aaaagaaatt
    21181 gttactactc ctgttgataa agccactggt tttgacgaaa ttattaatgc tgaaggtaaa
    21241 tttgtaatac caggcgtgat tgacgatcag gtgcattttc gtcagcctgg tttgacgcat
    21301 aaagcagata tttataccga atcgagggca gctgttgccg ggggggttac ttcctttatg
    21361 gaaatgccca atacccagcc tcagacaaca acacaacaat tgttgcagga gaagtttgcc
    21421 ttaggggccg aaaattcttt tgccaattac tctttttata ttggggcgac aaatgataac
    21481 ctttcggaga tagtaaaaac agatgtaaaa aatgtgtgtg gcgtaaaggt ttttatgggt
    21541 tcttcaaccg gaaatatgtt ggttgacaat ccgaaaactc ttgaagggat ttttaaagac
    21601 tcgcctttgc ttgtggctgt tcattgtgaa gatgagggaa caatacggaa caatcaaaaa
    21661 aagtatattg acttattagg agaagacctg ccctggcatt atcacccgat tatcaggagt
    21721 gcagaggctt gttaccgttc atcgtcattg gctgttgaga tggcccaaaa acataatacg
    21781 cgcttgcatt tattgcattt gtcatctgca aaggaaatgc gtttgcttag cggggcgcca
    21841 tcgacatctg aaaaacgcat tacctctgaa gtttgtgtac accatctttg gtttaccgat
    21901 gaagactacg agcggtatgg ttcattgata aaatggaacc ctgccataaa atatgccgaa
    21961 gaccgtgatg aattgtggag ggcgttgctt gatggccgtt tagatgttgt tgctacagac
    22021 catgccccgc atactattga cgagaaaagg aatatatata ccaaagcccc gtcgggtggg
    22081 ccattggtgc agttttcgct attggctatg ttacagttta cgcaacaaaa caggcttagc
    22141 atagaacaag tggttgaaaa aatgtgccat gcccctgcaa aaatatttca ggtaaaagaa
    22201 aggggttttt tgcgcccggg ttattatgcc gatatagcca ttgtaaaccc caatgaaaag
    22261 acacaagtta gaaaagaaat cattttttca aagtgcggat ggtccccatt tgagggggtg
    22321 ttgtttgatt attcagtatc tcaaactatt gttaatggcg ttttggttta caacaacggt
    22381 aaaataaatg agcatttcag gggaaaagcc cttacctttg aaaggtgaaa ttgtaatatc
    22441 agtagtaaga ttatttttta aacgtatttt tcaaatcaaa tatgggaagc aaaagtgata
    22501 tttcagaaaa acctgaagaa aaggtttcgt taaatttttt agaagagatt atagaagaag
    22561 acattcgcac tggcaagtac cagggccggg tattgacacg ctttcctcca gagcccaacg
    22621 gctatttgca tatagggcat gccaagtcta tatgcctgaa ttttgggtta gccaaaaaat
    22681 acaacgggaa atgtaattta cgttttgacg ataccaatcc tgttaaggaa gatacagagt
    22741 atgtagattc aatcaaagaa gatgttaatt ggttggggtt tcagtgggcc aatgagtttt
    22801 atgcttctga ttattttgat caattgtacg aatgggcaaa agtacttata aagaaaggca
    22861 aagcttatgt atgcgacctt tcggccgagg aaattagtgc tatgcggggg accccgacag
    22921 tacctgccac gcctagccca taccggaaca ggccaattga agaaaacctt gatttattca
    22981 aaaggatgaa aaaaggagaa ttcccggatg gttcaaaaac gctaagggcc aaaatagacc
    23041 ttgcatctcc caacatgcac atgcgcgacc cagttatgta ccgcattatg cacacacatc
    23101 atcatcgcac aggcgataaa tggtgtattt atcctatgta tgactatgcc catgggcaat
    23161 ctgattatat cgaaggcatt accaactcta tttgtaccct tgagtttgaa gtgcacaggc
    23221 ccttatatga ctggtttatt gacaatattt ttgaggaccc tgatggttac aggccccgtc
    23281 agtatgagtt tgcgcggctc aacctgagct atacggtaat gagcaaaaga aaactgctgg
    23341 aacttgtaaa aggaggatac gtaaaaggtt gggacgaccc tcggatgccg actatttgtg
    23401 ggttgcgccg tagaggttat actcctgaat caatacgcat gtttgccgaa aaggtaggag
    23461 tagcccgaag agacaatatc attgatgttg cattgctcga attttgtatc cgcgaagacc
    23521 tcaataaaaa ggccatgcgt gtgatgactg tgttaaaccc cataaaattg gtgatagaca
    23581 attatcccgc aggacaaacg gaaatgctcg aggctgttaa taatcctgaa gacgagtcaa
    23641 tgggcaacag gagcatcgag ttttccaggg aattgtttat tgaaagggac gattttatgg
    23701 aacccccccc aaagaaatat ttcaggttag caccaggcca ggaagtaagg ctccgatatg
    23761 catattttgt caaatgcacc catgttgaaa aagatgtaca aggtaatgta actgtagtgc
    23821 attgtaccta cgatccggcc tcaaaggggg gtaactctcc tgatgggcgt aaagttaaag
    23881 gcacaattca ttgggtaaat gccaatacag caatagaggc agaagtgcgg ttgtatgaca
    23941 ggttatttat gcagcccgag cccgagaagg tagaagaagg ccacgattat aaagagaacc
    24001 tgaaccccga ctctttaaaa gttataaaag cattttgcga gccatatctg gcaaatgcca
    24061 aggttggtgt taaatatcaa ttcgagagga ttggatattt ttcggtggac aaagattcga
    24121 caaacgacaa gcttgtgttt aaccggattg tgacattaaa agactcgtgg gcaaagatca
    24181 gtgcagaata atataacgag aaagcttttt tatattttat gtagcatatt ttgtaaatta
    24241 gtgtatcttt gcgcgctcaa ataattataa aacgttaagt tatgcccgca aaaattagat
    24301 taacgcgcca ggggcgcaaa aacattccct actaccacat tgtagtagca gacagcaggg
    24361 cgccacgtga tggcagatat attgaacgtt tggggaaata ttttcccaaa accaatcctg
    24421 cctctgttga gttaaatttc gacagggcat tgtattggct gcaaactggt gctgaaccta
    24481 cagacacatg ccgtagcatt ttgtctgagc aaggggtatt attaaaaaac cacctgctta
    24541 atggggttaa gaaaaaagcc cttactttag agcaagttga aacaaaattc agtgcctggc
    24601 ttgctgagaa agacaaaaaa ctggcagcaa agaaagactt gattaagagc gaagttgaca
    24661 aagccaaaaa acaacgttta gaagctgaat caaaagttcg tgaagctaaa gctgctgcta
    24721 tagcacaaaa agcaatgcag aagctcgaag ctcaggcaga agcccaagca gaaggcgaag
    24781 cccaggcaga ggctgaacaa gcataaaact gagttgggat gattgccgaa cctgttactt
    24841 tcatcggcaa atgcttaaga taccatggtt ttaaaggcga ggtggttatt gctttagagc
    24901 catcagttag aaagtatatt caaaaagcgg aatgggtttt tatcaatatt gataattcgc
    24961 ctgttccgtt ttttgttgag tccatatcgg ctactgacca gtctgccatt ttaaagctaa
    25021 agggcataga caaagaaaaa cagttgcccg aaatattaca gcaggatgtt tttgtcgcgt
    25081 cttcgttgtt ccccagtaag tttaaacctc agcaaaacga gttccagggt tttacagttt
    25141 tttcgcaaga aaaagaaatc gggacagccc atgagttagt tgactataat gggaatttgc
    25201 ttttgcaggt ttttaaccac gagaaagaaa tcctgattcc ggtgaacgac aatacgattg
    25261 tgtctgtaga taagcgcaag aaaaagattg tggttgaagt gcctgaaggc ttattggaat
    25321 tgtacttatg atttcttatt ttttaccact ttgttcattt ttagcgcaga gaataaagta
    25381 atgataaccc caaaggtaaa taaaataata gcgctgccca gataactgaa attgataagc
    25441 tttggttctt tttttatggc accgatagtg cctattattg agccagctat aatgcctatc
    25501 ccaaaaatca atgccagtat gacaataact tttgaatata catcagtgct ttcgtaaaag
    25561 ctggtttttg taaaatgttt gtatttcatt gaagcatgtt ggcgtgccct ttccctaatc
    25621 cgttctcgct ccttacgcag ccactggtct attatttcct gggcttcatg ttgattggcc
    25681 cctattttat cagcttttaa cctggcttga gccaataaat actcataagc ttcattgatt
    25741 aagataaaaa gttctgccgc atttctgaaa ttactcacat ccggatggta ttcgcgggct
    25801 ttgcgcctgt atgccctttt gatgtcatct attgatgcat ctttgcttac ttctaatatc
    25861 tggtagtagt ctttgataac ctctttttta acaaatatag tttttttgtg tgtaaaaggc
    25921 agaaagggca aaccttcttt tcgtttatca acaaaaaagt gacattcgtc actcgagatt
    25981 aacagtttgc caccatttta ccccccccat caaacaatta ataatcaatg cattaccgtt
    26041 atcggggcac ttttatattt gtcacttgtt tgctgaagcc acatttttgg cattgtagat
    26101 attaagaaga aaaaattaac acaaaatcta taatcatgaa acacccatga ctctgcaaat
    26161 cgccaacacg aggatatttt attgtgggtg catactatca ttccattaaa aattaaaaaa
    26221 taaatatgat gaaaaaacta ttactattca gttttctttt tgttagtttc agccttagtg
    26281 cacagataaa tgttttcagt ggcaataacg tcggcattgg ggccggttta acaagttctg
    26341 cttctaccct tagcgttaac tcggcaggtt ccagtagtta tgctgtgtgt gtttcttcaa
    26401 caagttcata ttcgggaggg attttttgcg gggtacaagg gtcaaacagt tcgccaagat
    26461 ttggtatttc aagctctata aacaaccttg gggatgggag tataaaaagg gcaatatatg
    26521 ccactgcatt tgatgctact gcacgaacag atggccaggc ttatggggtt gtaggtattg
    26581 ccggaaattc aacaaatggt tacaatgttg gtatattggg gcaattatat gggtccaata
    26641 atggttctgg tgtttacggg cagataggga cgagttttgt tgcccctgat gataaatatg
    26701 ccgggtattt ttatggcaag gttaaaatta atggaacatt atgggttaat agtactcaaa
    26761 ttaccagttc tgatgatagg ttaaaacaag atgtcagttc aattgaatcc gtcgatggcc
    26821 tttataagct aaaacctaag aaatataaat ataaaagcat caacgaacag gtaaaggcaa
    26881 tcactggcaa attaagtgag aatgctaatg acactactgt taataaagaa attgtcgatc
    26941 ctgaccccga catggcacaa aaaacacact ttggcttcct ggcccaagac cttcagcagg
    27001 tttaccccga gttagtttac caatcagctg acgggacgtt agggattgac tatcaggggt
    27061 taattcctat aatcataggg caaatgcaac aaatgaaaaa gtctattgat gataaagacg
    27121 agcagataaa agacctgaaa aagcgggtgg ccgcgcttga gaagaagaaa caataaatag
    27181 ctggtatgat gaaagcatta gtaaaaaagc tattgacaag tatagtactt gttaatatat
    27241 atatcgttgg acattcgcag gcatttaaaa gttcgcttga atatagctat gatgccaatg
    27301 gcaaccgcgt gaaagcttct gtgatatatt tggtcaaatc tgcaaaagag gctgtagagg
    27361 aaagcactga tactatacca cagataataa caaagaatat tgccactgac tcaattccgg
    27421 aaaaaggatg gggcctggcc cggaagataa tatatcgggc ggcaaaataa aactgtatcc
    27481 taatcctgct cacagtataa taattgttga gatttctaat tccgatttga acaagaaaga
    27541 acagtcaaat atgattcaga tatttgattt gcagggaaag atggtatatc ataaaacagg
    27601 cataacacga tactcggcca ttgacctgag tatgttgagc aatggtacct atattctcaa
    27661 acttcattta ggagaccagt taaaggaata taaaattgtt aaaaattaac ccctgaataa
    27721 ttcatgaaaa agattatact aacaatttat cttataatcc ttgtatttaa gacatatccg
    27781 caatgtaaca atgccaatca ggtcatttta gatggcactt gtgctgcaag tattaatgcc
    27841 gggctggtta caagtaacca aactgtagtg gcacgcgacc agataaccat gaaagccggg
    27901 ttcagtgtaa gttctgtaaa tggaaaaaaa tttgaggcta agattgacta tactaaagta
    27961 tatgtaacaa acgattattt gactggcaca ggaattgtta ccgggactag ccgcccactt
    28021 gataaaacaa attgtgcccc cgggtctgtg gggggctcaa ttaatgttag cccgacgggt
    28081 gcggcaacat ataatgtccc gattatagta tcgccaggca cgagggggat gcagcccaac
    28141 ctgagtattg tatataatag ccaaagtggc aatggggttc tgggttatgg atggcacctt
    28201 aacggagtgt caactatctc aaggacaagt aaaaacttgt attatgatgg cactacatct
    28261 ccggtaaaat ttgatgccaa tgatgccctt tctttagatg gtgccaggct gataaaaaca
    28321 gggacaaacc aatattcgcc cgagaacaat ccatatacag ttgtaactac cgatgcatct
    28381 tttagcttct ttatagtaac aacgcaagac ggggtgtcaa tggagtacag gcaaaagttc
    28441 ctgcctaaag gaggtgatat agcttatgcc tgggcaataa caaaaataac cgataccgaa
    28501 ggcaactaca tgcaatatat ttatcttggg gacaatacta ccggcgaata ctatatttcg
    28561 gaaatcaaat acacgggcaa tacgggcaaa gcgccttata actcggtaat ttttgcctat
    28621 gacaaacgaa ccgatgtcaa tacgaattat atttgtggaa aagaaacaaa caaaaccctt
    28681 ctgatgacat caattaaggt atttgctgaa ggtattttag cctacgatta taagttttct
    28741 tacttttatg atggctatta cagcaagctt aaccagattg gcttaacagc caacggattt
    28801 tcatataacc cgatggttat tgattggggg gcttcaccaa attttacaac aacaacacct
    28861 actacaaatt ctcttattat aagcgccccc tatatttccg ataattttgc ccagattgtt
    28921 ggagatatga atggagatgg cttacaggat tttgcccaga ttgttccttc agatgattat
    28981 ccccgtattg aagtagcatt tttgaaatca gatggttcgt atctattttc ttctgttaac
    29041 ttgaaaaaga aggtagtgag tggagcaggg gataactatg aagcaaaaga catgaatgta
    29101 gcagattggg ataatgatgg taaggatgag ataatggtac attacctttt ttataaaacg
    29161 aattctaaag gagatacctc gaattacaaa catttaatat ataagtatag ttgtagtggt
    29221 actacaataa ataatactcc agttttagat tgtacccttc ctgccggtag tataaaaaag
    29281 attgtgtaaa cactcataaa agtaaccaga taatgctgc
//
LOCUS       DAOSPF010000045        28761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1644_length_28761_cov_6.192294, whole genome shotgun sequence.
ACCESSION   DAOSPF010000045 DAOSPF010000000
VERSION     DAOSPF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28761
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_28761_cov_6.192294"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6003)
                     /locus_tag="PK252_09665"
     CDS             complement(<1..>6003)
                     /locus_tag="PK252_09665"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15014.1"
                     /translation="GTFNYSIALTGGCGTANATGTIVVNPLPTSNITGTTSICPSNSA
                     SLSIALTGTAPWSLVYTDGTTPKTVTNILTSPYEFSVSPSTTTTYTVTSLTDNNTCAA
                     TPAQMTGSATVTVLTPPTASISGSATICNGGNAPLSIVLTGTAPWSVTYTDGTTPVTV
                     NPIVSSPYNFNVNPSSTKTYVLSAVSDANCIAGPSGLTGSATVIVNPRPTSVISGSTN
                     ICQGSNATVSIALTGTGPWDLTYTDGITSVPVTNILTSPYTFTVSPAVTTTYTVTALS
                     DSKCVSNASDRTGSAVITVDPPTVGGTVSGSATVCSGTNSTLLTLSGHTGSVVRWQYS
                     TNGGVTWVNIANTNTTYTATNLTQTRQYRAVVQSGSCSPAYSSAATVTVVASSVGGTV
                     GTSTSVCYGINSTTLNLTGYTGSVVRWESSTNGGVTWTPIANITSSLTATNLTQTTQY
                     RAVVQSGSCPTANSTAAIITVYSDLNAGSIGSDQTVCENVVPTITETPASGSTGVYTY
                     LWQQSTDGGATWTNASGTNNLESYTAPAVTQNIQYKRQVTSGSCGTKESNVITVTMDA
                     LPHFTSQPIDQSSCVGGNVTFNVSVTGATSQKWQLSTNGGATFSDIPGETGNSLTVNA
                     VTLLMDGYKYQLVATNACGNQTSNVVNLAVVAATTISVQPSDAGICNNGDTAFTVVAS
                     GTGLTYSWEISTDGGTTYNPLVIAAPYSVTNTTTSSKLSLTGAQNTLDGNKYRCRVNS
                     ATCGNTLSNAATLKFYNIESITSRTDNSRCGSGSVVLSASASGTAQVLWFADLTGGAS
                     LASGSNFTTPSIAATTTYYVSADNNGCQSSPRLPVVATINALPLEKSINDATVCQGGS
                     VTLTLLNSENGVSYQLRDNSDNSNIGVAVDGTGADITFTFVPTSTLTANVLATNKTTS
                     CSLQMADMASVTVNPLPAQKALSNDTVICAGGSAILGLKASQNGVTYQLKQKSDNAVV
                     STKIGNGAYLQFDAVSPASTSSYYVTADNGCTVNMTDEITINVASVLTISASVNDVTG
                     CSTSLNGQITVNVLTGTATSYTLTPGITQPSNIFSGLAKGTYTVSATNGVCTASVPAT
                     VGGPAAISLSINPANSSVCSKDKLGYSATISNAVAPVTYAWSSTSGGFDDAASATPNY
                     SNPVAGDYTINLSVLDGNGCTASANTGVSVLACPTITLTAIPTSPTCFGYTDGKIVTS
                     VTGGTPPYTITAVPAIADFNNVAPGTYTLTATDALSNTDHVTVVVDPAQKLAATMTVI
                     NYDGTAGNGKVTVNATGGSGSYEYDFNNAGYVPSNVFSSLAAGTYSLKIRDINGCGEI
                     DTTVEVLDDSKLELWPPTWENVKCFGTPSATASIHIKTGKAPFTVRLRRGATTVALAT
                     VYAKDTTFTGLVAGTYQFNVRHSLPGASVNTPSFVIEDNNKSLLVIDSKTATPQTDVV
                     PNGTITILASGGTGNMTSYQFSKDNGKTFDVNGGEFDGLEGNKKYYIAVQDLVGCIIY
                     DSIVVPIDIKLNVDFSKTNETCAGLNDGTVTYNVTSGNGTYKYSFNGAPFVSAPVTKT
                     FTGLIPGTYPLLVTDGIDTASFSANILPASAVIIDSITTTVSANGNTGTMTVYVNPNS
                     GSGVFTFSAISLHHGSFNNGNNNVFTSLPADTFTVRVDDVCSFATGTAIVKQDTVLLG
                     KIDSVEDVTCIQPFLQAFINVSVSNAAAGPVYYKATNANDPSFPYEQYDDGHIEVNDT
                     GTYYIYVEDAEGKRYRDTAYVKAIVTPILIDLTPSKLLCNTGETGTIISTVSGGKAPY
                     KYEWYQNTTLVSKEKDLVDFGPGKYRLVVTDSFGCRRIEADSIFLNKVKIQKIEKTLS
                     TCNVGITNGTIKVTATVVSGEPLHYEWSNGLPDASYVTGLGAGVFSLKVSAASGCFDT
                     ATIKLASKDTLLSTWHYADFTCQDSASIGFTITKGKPAYKFITVGVDTFNTSLTTYSR
                     HIPEGTYKFKLEDSKGCYDTLEYIVHPLPANDSVIIDTVYTT"
     assembly_gap    6004..6103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6104..7213)
                     /locus_tag="PK252_09670"
     CDS             complement(<6104..7213)
                     /locus_tag="PK252_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15015.1"
                     /translation="MKSLLKLSLFILLCTIVTSNIFAQLPTIASVSIDSVSCNGFSDG
                     KIQVNITGGLPPYHYTISTGTGGVTTDTFYVFSGLPMGTYWIVVEDANGDGDIIFGVR
                     VRQPDVLSVSFPVSNYCLNVPTPIDALVSGGNKIYSYFWDGTAVDNGFLNQDDIEDPT
                     FTGKVAGNYNLRLTVIDNKGCTKATALSDIEVFNLPSVTFGGVLADQCANNTTYTLSG
                     GNPVGGSYSGPGVSGTNFNASASGAPGTKIITYTYTDANSCTNSASNTIEVLALPSVS
                     ISGTYPDQCVASTTYTLTGGTPVGGIYSGPGVSGTNFDASVAGVGTHTITYTYTDGNG
                     CSNTATKTITVVAANTAGAASSTPTLCINTALTPIT"
     gene            complement(8133..9014)
                     /locus_tag="PK252_09675"
     CDS             complement(8133..9014)
                     /locus_tag="PK252_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15016.1"
                     /translation="MENTNSSPNRKLTMILTILVVVLAASLGVLYMQYQKKMADNAIV
                     QEALEEQKESLTSELKDMMSEYEGLKSDNDSLNNQINKQQDRIKNLLAINASNLEKIK
                     LYKKELATLREVMKSYIIQIDSLNTKNQKLVAENIEVKTALDDARKNNESLSKEKADM
                     SSKIEVAKVLSAKNITVSPLNRRGKDTEKASRTAKIKTSFTIRENSLASAGEKTIYIR
                     ITRPDDLVLASSEQDVFEYEGNKIVYSAKRQVTYQNADLDVSLYWDNAGELIAGTYNV
                     DLFCEGKLIGSSSFSLK"
     gene            9242..11266
                     /locus_tag="PK252_09680"
     CDS             9242..11266
                     /locus_tag="PK252_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT15017.1"
                     /translation="MRNFILLIFSLMAMSHNIKSQSLKMGSIFADHMILQQKTKAPIW
                     GTAKPGSEINIIGSWNAKAKATADDKGNWITAIKTPEAGGPYTLSICSSTKSIEIKDV
                     LIGEVWLCSGQSNMEMPLTGWPPKDTIMGSAKEITNANNPQLRTFTVKRGYSLSPEKD
                     CKGEWTLFNKEHAGKFSATAYYFGKRLYEELKVPIGLLHTSWGGTPIESWTDGQTLQQ
                     IPEYIEYFVNLKKLSLEYDNFIIWVNKHHKMKLAALAEEEKWENLNFNDSACSTVNYN
                     DINWKTMTLPGYIEKTELGAFDGAVWFRRNISLTEDYAGKDLEISLGPIDDMDVVYFN
                     GIRIGGIEKTGAYQQDRIYPIPANIAHKGINTIAIRIVDNQGGGGIYGTTEKMFIRDK
                     QNENIKMSIAGEWKYLPVAEYLNDEFFIYPHENMEGYNRPGGSNAANAYQPSVLYNAM
                     IAPLVPYAIKGFIWYQGESNVGNAELYSRLFPAMINGWRQSWAQENLPFYYVQIAPYL
                     YSGENNIESAWIRDVQRRTLSVKNTGMAVTLDIGNVHNIHPCNKTDVGQRLALWALSK
                     DYKKNIKYSGPLFEKVSFEANKAIVTFTNAESGLKIKEGLPNEFEIAGEDGIFYPADY
                     SIAKNTIILNSAAVLKPVKVRYAFKNGSQGMLFNNEGLPASSFTSENKIE"
     gene            complement(11359..11748)
                     /locus_tag="PK252_09685"
     CDS             complement(11359..11748)
                     /locus_tag="PK252_09685"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT15018.1"
                     /translation="MENLYLNIYPELVYYPIVDFNYLTGECVISGESYMEETYKFYLP
                     VINWLKTYIAEKKPVVFTVKLTYFNTSSSRMILEILLILKQYQSEGGEVKVVWFYKSN
                     DPDMLMDIESFIKETGLAIEAVKVDKM"
     gene            complement(11766..12284)
                     /locus_tag="PK252_09690"
     CDS             complement(11766..12284)
                     /locus_tag="PK252_09690"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT15019.1"
                     /translation="MSKEQKDKRFYLSFEGPFNMRIISSSGRYFIENLSDAYETRISL
                     YRVFIEICQNVALYSAERFRQSNNNTLGIGRFVVEEKEDGYVCSTSNAIQPEHREKLC
                     KYCENLNKSNADELINLKSTLRHQSSLVATGAHIGLISIKLYSKNQLKYDFLKSSDDD
                     ISFNISAFISKS"
     gene            complement(12281..14032)
                     /locus_tag="PK252_09695"
     CDS             complement(12281..14032)
                     /locus_tag="PK252_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT15020.1"
                     /translation="MQLKIPKYLVVLVVMVIWGSAKGQDAHDLENLARGYEQQGNTIQ
                     AAAYFNKAGYSYWNSGHADKAVVVFRKALDLYILTGNNVACITVANNLGFVYSGDNRH
                     GEARKAFMESLRFARKMGNKNEVMNSLINISNSNIEEQRYNEAVENAKEALAIANELS
                     MLRSIARCNALLAEANEKLGNSSEAFKYYEAYSLIDKKMKAQEIDEIKSKSEEEIAKA
                     NLEKHVTALELKVKKGELKVVQDSLFVAMKMAMQSQFEVEKRNAVIKEKEYQLKLEKQ
                     IRLMLVLGITIALVLVVALVFFIFRKIRDNKILLLQKEEITAQRNKLDLQNKKITDSI
                     EYALRIQQAMLPNLLNVDFDMDVLYLPKDIVSGDFYWYYNANEVKVNYHFFAVVDCTG
                     HGVPGAFMSMIGNRLLNEIVATRKVYSPSQALQMINQMLHKELHQDISQNMDGMDICL
                     CRLAAIDKEHYELVFSGAKRPLYWVTAAGDAVLIEGDFITIGGIQGSAKKFTEIHKTI
                     RVGDSLILYSDGMIDQPNVFRARYGSERFLDFIKANIKDPASNLLGKLHDSFKEFKQD
                     EEQRDDITVFVIKTEIK"
     gene            14287..14844
                     /locus_tag="PK252_09700"
     CDS             14287..14844
                     /locus_tag="PK252_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT15021.1"
                     /translation="MNKELEDIIKACMAGDKNGQEALYKLFSGRMWAVCLRYAPDRDV
                     AKDVFQDAFIKVFEKIRQFQFKGNFEGWMRKVFVNTAIACYRNKNTLTYELSPNLLPE
                     ENDDREELTDSDISRDELMKMIEELTPQYKMVFNLYAIEGFSHKEIASMLSISEGTSK
                     SNLSRAREILKKKILTRLGTNIYAG"
     gene            14846..16216
                     /locus_tag="PK252_09705"
     CDS             14846..16216
                     /locus_tag="PK252_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15022.1"
                     /translation="MSENNIDRIFKEKLYDHAALPPSDSWSNIEDALHNHKKSRFISI
                     YWAMAASLALLVGIGTGFYIGINHQKDIGNSIASNNKTNTIKKIKTNEIKTVSNNQTI
                     ENPVISEKNANNKSITFTNSKNTADNILEEKTNIETTTVITKNTEVEKDVYEQGQTTL
                     LAYQESKSITNLKNTLNYELCLNTLYAMEEKERPETENVSKTQYFIGVNWTPLYAYRN
                     IGYNNTSISTTTYDKSEKPIYSFSGGLSFGIIHNRWYFQTGISYTQMGQQIPNASVPS
                     TKRNTYLAGDDASWTSSVSIPKYEATANSMGNFEAKELENSEAYANVKSEATYRSESQ
                     VSTVKYLINYSFIEVPILASYKVSTRLIDFRFTGGASLNFVINNRVKLFNGSIKENIG
                     TNSNAAPFNLAGIIEWGIDLPMTSKLHFVVGPQLRYFLNSINKSTNIKTYPYSIGINA
                     TLQYHL"
     gene            complement(16207..17142)
                     /locus_tag="PK252_09710"
     CDS             complement(16207..17142)
                     /locus_tag="PK252_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT15023.1"
                     /translation="MKIVFLDTKTMGNVSNLYLLEQFGEVVYYPLTPAELTAQRTADA
                     DIVITCKVVIDQKVIDASPSLKLICAAATGINHIDSKYAISKGIEVCNVSDYSTNSVA
                     QTTFAHILSLLNHVRYYDQFVKDGSYSKYDMFSHMDMEIGEIYGKTFGIIGLGAIGRK
                     VALIAKAFGANVCYYSTSGNNNNPDYKKVSLDELLIISDIITIHAPLNNNTINLIDID
                     KLKLMKPSAILINVARGGIVVEKDLAFAIDNKIIAGAATDVFSVEPVKADNPLLHVFN
                     KDKISFSPHCAWTSIEARTKLIEGIAQNIRRFLYR"
     gene            complement(17146..17793)
                     /gene="folE"
                     /locus_tag="PK252_09715"
     CDS             complement(17146..17793)
                     /gene="folE"
                     /locus_tag="PK252_09715"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT15024.1"
                     /translation="MDNDFIQIMGEGTINDDEYAKIEQFSAARTQKLEELYKGVLDVI
                     DPEPTREGLEKTPIRVAKAMQFQTQGYNIDPRKILQSATFIEDYKQMVIVKDIELYSM
                     CEHHMIPFFGKAHVAYIPNGKITGLSKIARVVDAYSRRLQVQERLTMQIRDCIDEGLR
                     PLGVAVVIEAKHLCMMMRGVQKQNSVTTTSAFTGAFLNDTKTREEFIHLIGTKLH"
     gene            complement(17786..18202)
                     /locus_tag="PK252_09720"
     CDS             complement(17786..18202)
                     /locus_tag="PK252_09720"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT15025.1"
                     /translation="MSLVYLTRRERFNAAHRMFRPEWDDTKNLEVFGKCSNPLWHGHN
                     YVLYVTVKGLPSQETGCVVNLSHLSQLIRNEIIEKLDHKNINLEVDFMKGKIVSAENL
                     AIAIWNELYDKIAALGIELHCIRLEETENNTVEYYG"
     gene            18400..19098
                     /gene="mqnB"
                     /locus_tag="PK252_09725"
     CDS             18400..19098
                     /gene="mqnB"
                     /locus_tag="PK252_09725"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOT15026.1"
                     /translation="MKKILIISATEKEINLLKQNLQGLTQVNAWTFSGKFNMLDIDIL
                     IGGVGIPANVYRLTKRLSEQHYDLVINMGIAGSFSEKIRNGDVVNVTKEYFADIGIDD
                     NGQFKNIFEMGFLDKNTPPFVNGALNNPFTFRKFEIFSKIPNVTGITVNTTSGSEGCI
                     VNRWIKYQPDVESMEGAAVFYVCLQAKTPFIELRSISNKVEPRDVKKWDVPQAIISLS
                     NHVMFLLSEINSKE"
     gene            19095..19925
                     /locus_tag="PK252_09730"
     CDS             19095..19925
                     /locus_tag="PK252_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOT15027.1"
                     /translation="MNLSIAYSPCPNDTYIFDALAHRKISMEALSFECFLADVEELNR
                     AAQIATYDISKISIAAYPSIANKYQILNSGSALGRNNGPLLISKKPFTTKDINSLHIA
                     IPGKHTTANMLLSIAFPNAKNKTPMLFSDIENAVLKGEVDAGLIIHESRFTYQDKGLK
                     KVVDLGEYWQKTFDMPVPLGCIVVKRSLNNEIKKSIDNIIYRSLDFANKNKQSSQAYI
                     KEHAQNMGDDTIAKHIGLYVNDFSLSLGPKGKDAILFMLKEGEKAGLLSLIRDDIFID
                     "
     gene            complement(19922..21796)
                     /gene="ispG"
                     /locus_tag="PK252_09735"
     CDS             complement(19922..21796)
                     /gene="ispG"
                     /locus_tag="PK252_09735"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOT15028.1"
                     /translation="MSLSFKYCNSLTEFSRFVTKEVNVGGVCMGGSNPIRVQSMTNTP
                     TLDTNATVEQCIRIVNAGADYVRITAPGVQDAQNLAAIKSSLRKRGYKTPLIADIHFN
                     PQAAEVAAAIVEKVRINPGNYADKKRFELLEYTDVEYKLELERIRERLLPLLRICKEH
                     GTAIRIGVNHGSLSDRIMSRYGDTPMGMVEAAMEFLRICKNEGFDQLVVSLKASNTRI
                     MVQAYRLLVATMMAEDMNYPLHLGVTEAGDGEDGRIKSAVGIGALLADGLGDTVRVSL
                     TEVPELEIPVAKNLVDYFSGRKTNPAIDVVCEEQKNPFEYKKRESIAVENVGGNNTPI
                     VIVDYQSDITNAAHLPAEYVFFATRAESIVLKDNKRYITSMNDWFKYFREKKNVFPFY
                     TAAQYLFYGPKHPHLNFVVLSLSEMDERMIEALKDDRRTVVIVETFNTNGVAEQRALL
                     YKMLAASLTNPVIINRNYCEYNLEMFQIKAAADVGLLLIDGLGDGIWIRNAAESVQKS
                     ICALSLGILQATRLRMSKTEYISCPSCGRTMFDLQATTARIKQRTSHLRHLKIGIMGC
                     IVNGPGEMADADYGYVGAAAGKVTLYKGKEVVRKNIPSEEAVDQLIDLIREHGDWVEP
                     "
     gene            complement(21793..22668)
                     /gene="dapF"
                     /locus_tag="PK252_09740"
     CDS             complement(21793..22668)
                     /gene="dapF"
                     /locus_tag="PK252_09740"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT15029.1"
                     /translation="MDKKNFFVKSHGLGNEYIVFDCSNITFDINTPNIEKICNIHFGI
                     GSDGILLVESSSVADFKFRVFNPDGSEAEKSGNGLRIFAKYIFDYGYTCKRHFTIETM
                     AGIVEATINKERNGKAEIITIDIGKAIFEPKQIPVNCDLPECIDFPLTIDGKIFKINC
                     ISVGNPHCVIVTDTLDEQLVKEFGPKIENHPMFPNRINIQFVKVLSDKAIQMLIWERG
                     AGFTLASGSSSSAAATMMHKKGLVGKEIDVNMLGGQLHISVDESWNIRLCGEVQQVAE
                     GWLSEEYVESLIKNI"
     gene            22846..24804
                     /locus_tag="PK252_09745"
     CDS             22846..24804
                     /locus_tag="PK252_09745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15030.1"
                     /translation="MQLSVVIVNYNVKYFLEQCLHSVYKAIKGIDAEVFVVDNNSIDG
                     SCSMVKEKFPWVNLICNNKNTGFSYANNQAIRISKGKYVLLLNPDTVVQEDTFSKCIA
                     FMENNPQAGAIGVKMIDGKGNFLPESKRALPTPEVAFYKIFGLARLFPQNRRFGQYHL
                     SYLDKDKTNEADILCGAFMFMRKEALDKSGLLDDSFFMYGEDIDLSYRIIKAGYKNYY
                     FADTTIIHYKGESTKKGSLNYVLVFYQAMIIFAKKHFSNRRFKMFNLLIHLAIYFRAL
                     LSIVKRVAKALYLPLIDAVSIYAGYLFVIRPFSESMKYEQGGHYPFLYIGLIVPLYIL
                     IWLISMILAGTYDKPIKITNIFKGIGIGTLIILAIYGLLPLDYRFSRLMIILGAVWSL
                     GTISFIRTILHLTGFKDFRLYNTNKKKIIIVGKYEEYLRVRQLLLQTSLIHDITGFID
                     ETNNESPDYLGNSSQLKDIIYIYKADEIIFCAKNLSSQDIINYMLNFSASGVEFKIAP
                     PESLSIIGSNSINTAGDLYLININAVNRPENLRSKRLFDFSISLCLLLTYIFIFPFVK
                     NKIKGLYNILLVLAGSRTWIGYTSDETQNRFSLPKLKKGILSPVDGLLKKDLSSETIE
                     KINFLYAKDYKVINDFNILWHGLANIGR"
     gene            complement(24832..25251)
                     /locus_tag="PK252_09750"
     CDS             complement(24832..25251)
                     /locus_tag="PK252_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT15031.1"
                     /translation="MKHIVNTTWKGNMLFDALVSGHHVKMDATPEHGGNDEGSRPKEL
                     MLASLAGCTGMDVISILKKMRVEPEKFDIAVEGELTEEMPKHYAAMHIIYKFWGKGLD
                     KEKLQKAVDLSQERYCGVSAAYKKAMKITFEIQVFEE"
     gene            complement(25366..26046)
                     /locus_tag="PK252_09755"
     CDS             complement(25366..26046)
                     /locus_tag="PK252_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15032.1"
                     /translation="MKKLVLMGTVLFCASIGINAQKCNMYFPSREGSESEMTSYNAKD
                     KIEGVTKTVILKKETGANGVSLLFESVYSDNSGQEKGKGQYKVRCHNGVFYFNMESMM
                     PQQNGDQASMEVKSDELEFPANPQIGQTLKDAKMTMVMKSGNVTVMEMSMSITNRKVE
                     SYEEITTPAGTFNCYKVTYNVRTHAMFDVEVKAVQWVAEDVGVVKTENYDNKGKKVNY
                     SLLTAFRP"
     gene            26363..27034
                     /locus_tag="PK252_09760"
     CDS             26363..27034
                     /locus_tag="PK252_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15033.1"
                     /translation="MKKTLLFLLSCLFLVLMSCSKDDDKDSEDGTTDTPQSTDGKVTF
                     LKKYEVKSGIIKFTEYSDILDETTTYKVFFDEYGGKEVKYEYDAKGALTDITMEKGDG
                     WYYQISIKNGGTKTSSPYADGTEMKFDLNWTEKQKTEYNFKMLSDTTIAGKVCKRYSM
                     DASESISGIYAGYKGVTLYQKSISKGSFTFYVEAKAVSFEENVAINDTVWKLPANITI
                     TESKY"
     gene            27233..>28761
                     /locus_tag="PK252_09765"
     CDS             27233..>28761
                     /locus_tag="PK252_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15034.1"
                     /translation="MKTHSTLRNLAIKAIMLALLWVPGTLFAQIVYISPSGSDLTGNG
                     SYDMPFKTLQKALDNCNNNIISWNIYLFDGTYDFDTTTISIASQSINSISAINTNGAT
                     IKNSKLIFDFAQTNQYTSFINLVIDSSSIEFSGAGTVYISDNHFKNSTYILAKNNTIT
                     ADVLNSIFENSGMELNSLSATIINNTFVRSLSVTGSYAIYVPQAVNISIEGNIFKGYD
                     YAIDGTAQNVKYNCFFNNVTNYPSSLDSSNIYSDPMLNSDFSLKPGSPCIGAYEGSDI
                     GAITYVTPVCNFDFTYVNNYGANYTFTPQPTNCNTFNSLTWTVRDPNNILIKTSTDNI
                     FTYTMPEIGYYTITLEGLTVNSETYTTSQYVNVYNTCPANGSMNIISADEITGLVKFK
                     ISAINTDSVFFSFGDLKDSVFKYSGTEMTFTHKYPTNWKYGNEFYPSYSLYNTLENCS
                     IPKYSDIPVSIKNKNCRITTWAYSDTLTLSVNAEKYGNEDVLVTLKNSKGTLDNFTLN
                     T"
BASE COUNT         9483 a         5760 c         4884 g         8534 t
ORIGIN      
        1 tagtagtata aaccgtatca ataataaccg agtcattggc tggcaatgga tggactatat
       61 attcaagggt atcataacaa cctttgctgt cttccagttt gaacttataa gtaccttcag
      121 gaatatggcg actatatgtt gtcagcgatg tattgaaggt atccacacca acggtaataa
      181 acttatatgc aggttttcct ttagtaatgg taaatccgat agatgcactg tcctgacaag
      241 tgaaatctgc ataatgccag gtcgatagca aagtatcttt agaagccagt ttaatagtag
      301 ccgtatcaaa acacccagat gcagcagaaa ctttcaacga gaacactccg gcacccaaac
      361 cagttacata agaggcatca ggcaacccat tgctccattc gtaatgcaat ggttctccac
      421 taaccacagt agcagtaacc tttattgttc cattagtaat acctacatta caagttgaca
      481 gtgttttttc aatcttttga atcttgactt tgttgaggaa gatagaatca gcctctattc
      541 ttctacaacc aaagctatca gtaacaacca acctatactt tccaggtcca aaatcaacca
      601 ggtctttttc tttagaaacc agtgttgtat tctgatacca ttcgtactta taaggtgctt
      661 taccacctga aacagttgaa ataatagtac ctgtttctcc agtattacat agtagtttag
      721 aaggtgtaag atctatcaat ataggcgtga caatagcctt aacgtaagca gtatctctat
      781 aacgcttacc ctcagcatcc tcaacataaa tataatatgt acccgtatca ttaacttcaa
      841 tatgaccgtc atcatattgt tcataaggga atgaagggtc attagcattt gtagccttgt
      901 aataaacagg acctgccgca gcattactta cgctaacatt aataaatgcc tgtaaaaatg
      961 gttgaataca ggtaacatct tcaaccgaat caatcttacc taacaaaaca gtatcttgtt
     1021 ttacaatagc agtacctgtt gcaaaagagc aaacatcatc aaccctaaca gtaaatgtat
     1081 ctgctggtaa actggtaaat acgttgttat ttccgttatt gaacgaacca tgatgtaatg
     1141 aaattgctga aaatgtaaat actccagaac ccgaatttgg attaacatat acggtcattg
     1201 tcccggtatt tccatttgct gaaacagttg tagtaatact atcaataatt acagccgaag
     1261 caggcaatat atttgccgag aaagaagcag tatctatacc atctgtaact aacaaaggat
     1321 atgttcccgg aataagccct gtaaaagttt tggtaacagg agccgaaaca aatggtgctc
     1381 cattgaacga atacttataa gtaccattac ctgaagtaac attatacgtt accgttccat
     1441 catttaagcc tgcacaagtc tcattagttt tactaaagtc aacattgagt tttatatcta
     1501 taggaacaac aatagaatca taaataatac aacctactaa atcctgaacc gctatataat
     1561 acttcttatt tccttccaat ccgtcaaact caccaccatt aacatcaaaa gtcttaccgt
     1621 tatcctttga gaattgatat gatgtcatat tgccagttcc ccccgatgcc aaaatagtaa
     1681 tagttccatt aggcacaaca tcagtttgag gagtagctgt tttactatca atcactaata
     1741 atgacttatt attatcttct attacaaaag aaggagtatt aacagaagct cctggcaggc
     1801 tatgacgcac gttaaactga tacgtccctg ctacaaggcc tgtaaatgtg gtatctttgg
     1861 catatacagt agccaaagca acggttgttg caccacgtct taaccggacc gtaaatggcg
     1921 ctttcccagt tttaatatga atagatgctg tagcagaagg cgttccaaaa cattttacgt
     1981 tctcccatgt aggaggccat aattctaatt tagaatcatc caatacctct acagtagtat
     2041 caatttctcc acaaccatta atatcgcgaa ttttcaaact gtaggtacca gctgctaaag
     2101 agctaaatac gtttgaagga acatacccgg cattgttaaa gtcgtattca tagcttccgc
     2161 taccgccagt tgcattaaca gtaactttac cattaccagc agtcccgtca taattaatta
     2221 cagtcatagt cgctgctaat ttttgtgcag gatcaacaac tacagtaaca tgatcggtat
     2281 ttgacaaagc gtctgtagct gtcaaagtat aagtacccgg ggctacattg ttaaagtctg
     2341 caatagccgg aacagctgtt attgtataag gcggggttcc acctgtaacc gaagtaacaa
     2401 tctttccgtc agtataaccg aaacaggttg ggcttgtagg aatagcagta agagttatcg
     2461 ttggacaagc aagaacagaa actcctgtat tagcagaagc tgtacaacca ttaccatcca
     2521 gcacactcaa attaatagta taatctccgg caactggatt tgaataattt ggagtagcag
     2581 aagcggcatc atcaaaacct ccacttgtag aggaccatgc ataagtaaca ggagctacag
     2641 cattggatat agttgcagaa tatccaagtt tatctttact acaaactgaa ctattggcag
     2701 ggtttatcga aagcgaaatt gcagccgggc caccaacagt tgcaggaaca cttgctgtac
     2761 aaactccatt ggttgcagat accgtatatg ttcctttagc taaaccagag aaaatatttg
     2821 atggctgcgt gataccagga gtaagggtat agctggttgc tgtacctgtc agcacattta
     2881 ctgtaatctg gccatttaag ctggtagaac aaccggttac atcatttaca gaagcagaaa
     2941 tggtcaaaac cgaagctaca tttatcgtaa tctcatctgt catattaact gtacagccat
     3001 tatctgctgt cacataataa gatgaagtac ttgctggaga cacggcatca aattgtaaat
     3061 aagcaccatt gccaattttt gtcgaaacaa cagcattatc agatttctgt tttagttgat
     3121 aggtaacacc gttttgagaa gcctttaaac ctaaaattgc agagcctccg gcacaaataa
     3181 cagtatcatt gcttaaagct ttttgagctg gcaacgggtt aacagttact gatgccatat
     3241 cagccatttg caaagaacat gatgttgttt tattggttgc caaaacattg gcagttaaag
     3301 tagaagttgg cacgaatgtg aaagttatat cggcacctgt tccatctaca gcaacgccaa
     3361 tatttgaatt atcactatta tctcttaatt ggtaacttac cccgttttcc gaattcaaca
     3421 aagtcagggt tacagaacct ccctggcata cagtagcatc gtttatactc ttttctaaag
     3481 gcaaagcatt aattgttgct actactggca aacgtggtga tgattgacaa ccattattat
     3541 ctgctgaaac atagtatgtt gttgtcgcag caatcgaagg agttgtaaaa ttgctacctg
     3601 aagccaaact tgcaccacca gttaaatcag caaaccataa cacctgggct gtacccgatg
     3661 ctgaagctga aaggacaaca gaacccgacc cacaacggct attatcagta cgtgaggtta
     3721 tgctttcaat gttataaaat ttcaatgtag cagcattact caatgtattg ccgcaagttg
     3781 ccgaatttac ccggcaacgg tatttattac catccaaagt attttgagct ccggtcagac
     3841 taagctttga tgatgttgtt gtattggtta cactatatgg tgcagctatt actaatggat
     3901 tgtaggttgt acctccatca gtagaaattt cccagctata agttaatccc gttccagaag
     3961 ctacaacagt aaaagccgta tccccattat tacaaatacc agcatcagaa ggttgtacac
     4021 ttattgtagt tgcagcaact acagccaaat taaccacatt agaagtttga ttaccacaag
     4081 cattagttgc taccaattga tacttgtaac catccataag caatgtaaca gcattaacag
     4141 ttagcgagtt accagtttct cccggaatat ctgagaaagt tgcacctcca ttggttgata
     4201 gttgccattt ctgtgaagtt gccccagtaa cactaacatt aaaagtaaca ttgcctccca
     4261 cacatgaaga ttggtcgatt ggctgcgaag taaaatgggg caaagcatcc atggtaacag
     4321 taattacatt agactctttt gttccacaag agccactggt tacctgacgc ttatattgaa
     4381 tattctgagt tacagcagga gctgtatatg attctaaatt attggtccca gaagcattcg
     4441 tccaggttgc accaccatca gtcgattgtt gccacaaata ggtatataca cctgtcgagc
     4501 cagaagctgg tgtttctgta atagttggaa caacattctc acaaacagtc tggtcactgc
     4561 caatagatcc ggcattcaaa tcagaataaa ctgttattat tgcagcagtt gaatttgctg
     4621 ttgggcaaga accgctttgt accactgccc tatattgtgt agtttgcgtt aaattagtag
     4681 ctgttaatga agaagtaata ttagcaatag gtgtccaggt tacccctcca ttggttgatg
     4741 actcccaacg tacgacactt ccggtatatc cagttaaatt caaagtagtt gaattaatac
     4801 cgtaacaaac agatgtactg gtcccaacag tacctcctac tgaagatgca acaactgtta
     4861 cagttgcggc agatgaataa gcaggcgaac aagaaccact ttgtaccaca gccctatact
     4921 gacgggtctg tgtaagattt gtagctgtat atgttgtatt tgtatttgca atatttaccc
     4981 atgtaacacc tccgttggtc gaatattgcc aacgtacaac acttcctgta tgtcccgaaa
     5041 gtgttaataa tgtagaatta gttcccgagc aaaccgttgc actaccagaa actgtacccc
     5101 ctactgttgg tggatctaca gtaatcacag ccgaacctgt acgatccgaa gcatttgaca
     5161 cacatttact gtcactcaaa gctgttacag tataagtagt agtcactgcc ggcgatacag
     5221 taaaagtata tggactggtt agaatattgg tcacaggcac agatgttata ccatcagtat
     5281 aagtcagatc ccaagggcca gttcccgtaa gcgcaatact taccgtggcg tttgaaccct
     5341 gacaaatatt tgtgctccca ctaataaccg atgttggacg aggattcact attacagttg
     5401 cactacctgt taatcccgaa ggccctgcaa tacaatttgc atctgaaaca gcagataata
     5461 catatgtttt tgtagaagaa gggttaacat taaaattata aggcgatgaa acaattggat
     5521 tgactgttac cggagttgtt ccatcggtgt aggttacaga ccaaggtgct gtacctgtta
     5581 atactattga cagcggggca ttccctccat tacaaatagt agcagaacca ctgattgatg
     5641 cagttggagg agttaaaaca gttacagttg cgctacctgt catctgggca ggagtagctg
     5701 cacaagtgtt attatcagtc aacgaagtaa cagtatatgt tgttgtagtc gaaggactaa
     5761 cactgaactc ataaggactg gtcaaaatgt tagttactgt ttttggcgtt gtaccatcgg
     5821 tataaaccaa agaccaggga gctgttccgg taagcgcaat actcaacgaa gcactgtttg
     5881 aaggacaaat cgaagttgtc cctgtaatat ttgatgtagg aagagggtta acaacaattg
     5941 tccctgtagc atttgcagtt ccacaacctc ctgtcaatgc tatactataa ttaaatgtac
     6001 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgttata ggtgttaagg
     6121 ctgtgttgat acacaatgta ggagttgaag atgctgcacc tgctgtattg gcagcgacta
     6181 ctgtaattgt ttttgtagct gtatttgaac aaccattacc atcagtatat gtataagtta
     6241 tcgtatgcgt tccaacacca gcaaccgatg catcaaagtt tgttcctgaa acaccaggcc
     6301 cgctataaat tccaccaaca ggtgtacctc cggttaaagt ataagtagta gaagcaacac
     6361 actgatcagg atatgtacca ctaatagaaa ctgaaggtaa tgctaatact tcaatggtat
     6421 tagaagcact attggtacaa gaattggcat ctgtataagt atatgtaata attttagttc
     6481 caggcgctcc agatgcagaa gcattaaaat tagttcctga tactccaggc ccagaataag
     6541 aacctccaac aggatttcca ccagaaagag tgtatgttgt gttgtttgca cattgatctg
     6601 ccaatactcc tccgaatgtt acagaaggaa gattaaacac ctcaatatcc gacagggcag
     6661 ttgctttagt acaaccttta ttatcaataa cagtaagcct taaattgtaa tttcctgcaa
     6721 ctttcccggt aaaagtagga tcttcaatat catcttgatt taagaaacca ttatctacag
     6781 cagttccatc ccaaaagtat gaataaattt tatttccccc cgacactaaa gcatcaatag
     6841 gtgttggaac attcaagcaa tagtttgaca cagggaaact aacacttaaa acatcaggct
     6901 gtctgacccg aactccaaaa ataatatcgc catcgccatt ggcatcctca acaacaatcc
     6961 agtaagtccc cattggaagg ccactaaaaa cataaaatgt atccgtagta acacctcctg
     7021 tccctgtaga aattgtataa tgataaggag gaagccctcc ggttatattc acttgtatct
     7081 ttccatcgga aaaaccgtta caagaaacag aatcaataga aaccgaggca atagttggca
     7141 actgtgcaaa aatattgctg gtaactatgg tacacaaaag aataaataac gaaagtttca
     7201 ataaagattt cattctttct tatttttaag ttacaaaatt cttaaaacat taataataac
     7261 tagccttccg aatgtctaaa ttttatctga aaaaaaatat cgcttactcc taattttgaa
     7321 ataatgcaaa cccagtcggg gcaagttatt aacaaaacaa ggccgaaaat taacattttt
     7381 tacgtaaaaa atgcaaatat gttcgattaa aaaaaagcct gataacatta acagttggtc
     7441 ttaaaaaaca ccgctattgc ttattgtaaa agcaatacaa ggaactttca gacttacaat
     7501 attttacaca aatagttaaa aaaaattgac gaaaatcacc tatgtattga ctttttttta
     7561 tctaacaaag cttttaaaca aacctttgat tgtaattatt agaaatataa aatctgaaag
     7621 accaacaaaa acaagtaaat aataatgaag atgaaagcaa aactaagagc tgtaaaaatt
     7681 tttaagccat aactacatct atccctgtaa atttaactta aagagattat aataggcctt
     7741 tacacaataa agagaaaatt tcaaatagcc ctgagccttt ggcaaaaact tgctgagtct
     7801 gcagggaaag tttacagttt tcttcaaaaa ggaattttta aagttcaaca ctcactgtga
     7861 taaaagaatt tttgagatat tagttaaacc cagattaatc attagtaggc tttttagagg
     7921 caaactatat gatttagttg ggttaatcct ctcgttataa acgagacaag ctgatttaga
     7981 aattatctta aaaattttta ttaagctatt gaattacaac ctcttaatcg aataagcatt
     8041 tttaatgctt aatttgggtt aaaagcttat ttcaataaaa aaaggctgtt agtacgaaac
     8101 agccttttaa aatattgtat attaaaactt aattatttca acgaaaagct agagcttcca
     8161 ataagtttac cttcacaaaa aagatcaaca ttgtaagtac cagcaataag ttctccggca
     8221 ttatcccaat aaagactaac atcaaggtct gcattctgat aggtaacctg acgtttagca
     8281 gaataaacta ttttattgcc ttcatattca aacacatctt gttctgaaga ggccaataca
     8341 aggtcatcgg gacgggtaat tcttatataa attgtttttt caccagcaga agcaagagaa
     8401 ttctcccgaa tagtaaaaga tgttttaatc tttgctgtac gagatgcttt ttcagtatct
     8461 ttccctcgac ggttaagtgg tgaaactgta atattttttg cactcaacac tttagcaacc
     8521 tctattttag acgacatatc ggctttttct ttcgaaaggc tttcattatt tttacgagca
     8581 tcatctaaag ctgtttttac ttcaatattt tctgctacaa gtttttgatt ttttgtattg
     8641 agcgagtcaa tctgaatgat ataacttttc attacttcac gcaaagtagc cagttctttt
     8701 ttatacaact taattttttc aaggttactg gcatttatag ccaaaaggtt ttttattctg
     8761 tcctgttgtt tgtttatctg attatttaag ctatcattat cacttttcaa gccttcgtat
     8821 tcagacatca tatctttaag ttcactggtc aaagattctt tctgctcctc aagggcttcc
     8881 tgtactattg cattatctgc catttttttc tgatattgca tatacaaaac acctaatgaa
     8941 gcagcaagca caacaaccaa aatagtaagt atcatcgtta gtttacgatt gggagacgaa
     9001 ttggtatttt ccatattttt tgtttttaag ttttatgggt gtaaatttaa tcataataac
     9061 taattgcaag aaaaaatatt tttctgaaag aaatataaat gtttatttct acctaatttt
     9121 gtttattgac cttttatttt tataaattat ctaacaaata aaaagacttg ataaatcatg
     9181 gcctgaatac gcaatatttt atatttgcca gactaaataa tcacacctta atttatatag
     9241 tatgagaaat tttatcctcc tgattttttc tctcatggca atgagccata atattaaaag
     9301 tcaatcgctt aaaatgggca gtatatttgc tgaccacatg atattacaac aaaaaactaa
     9361 agcgcctata tggggtacag ccaaacccgg ttctgaaata aacatcattg gcagctggaa
     9421 tgcaaaagca aaagctacgg ctgatgataa agggaactgg ataactgcca taaaaacgcc
     9481 tgaagcaggc gggccttata cattatctat ttgtagttca accaaaagta tagaaataaa
     9541 agatgtttta attggagaag tatggttgtg ttccggacag tctaatatgg aaatgccttt
     9601 aacaggctgg ccccctaaag acactataat gggctctgct aaagagataa ccaatgccaa
     9661 caatccccaa ttgcgtacat ttacagtaaa aaggggatat tcactctcgc ctgaaaagga
     9721 ctgtaaaggc gaatggactt tattcaacaa agaacatgca ggtaaattca gtgctacagc
     9781 atactatttt gggaaaagac tttacgaaga attaaaagta ccaatcgggt tacttcacac
     9841 cagttgggga gggactccga tagaatcatg gacagacggg caaacattgc aacaaatacc
     9901 cgaatatatt gagtattttg tgaacctaaa aaaacttagc ctcgaatatg acaactttat
     9961 tatctgggtc aacaaacacc acaaaatgaa gttagctgca ttagcagaag aagaaaagtg
    10021 ggaaaaccta aatttcaatg atagtgcatg cagcactgtt aactacaacg acataaactg
    10081 gaaaaccatg acattacctg gctatataga aaaaacagaa ctgggcgcat ttgacggtgc
    10141 tgtttggttt aggcgcaaca taagccttac agaagattat gcaggtaaag accttgaaat
    10201 atctcttggc cctattgacg atatggatgt tgtatatttt aatggcatcc gaattggcgg
    10261 gatagaaaaa accggagcat accaacaaga cagaatatat ccgattccgg caaacattgc
    10321 ccataaaggt attaatacca ttgccattag aatagttgat aaccagggag gtggcgggat
    10381 atatgggaca acagaaaaaa tgtttatccg agacaaacaa aacgaaaaca ttaaaatgag
    10441 tattgccgga gaatggaaat atcttccggt agcagaatat cttaatgatg aattttttat
    10501 ttatccgcat gaaaatatgg aaggctataa ccggcccgga ggaagcaatg cagccaatgc
    10561 ttatcaacct tcagtcctgt acaatgctat gatagccccc cttgtccctt atgccattaa
    10621 aggttttatt tggtaccagg gtgaatcaaa tgttggtaat gctgagttat attctcgatt
    10681 gtttccggct atgattaatg ggtggcgaca aagttgggct caggaaaact tgccatttta
    10741 ctatgtacaa attgccccat atttatactc aggagaaaat aacattgaga gtgcctggat
    10801 acgcgatgta caacgcagga ctttatcagt aaagaacacc gggatggcag taactcttga
    10861 cataggaaat gtgcacaaca tccacccttg caataaaacc gatgtaggac aacgcttagc
    10921 tctttgggcg ctatcaaaag actataaaaa gaacataaaa tactcagggc cattgtttga
    10981 gaaagtatct tttgaagcaa acaaagctat tgttacattt actaatgccg aatctggatt
    11041 aaaaatcaaa gaaggactgc ctaacgagtt tgaaatagcc ggagaagatg gaattttcta
    11101 tcctgctgat tatagtattg ccaaaaacac aataatacta aatagtgcag cagtattaaa
    11161 acctgtaaag gtcagatatg cctttaaaaa cgggtcacaa gggatgttat ttaacaatga
    11221 agggttacct gcttcaagct ttacaagtga aaataaaata gaataacaga gacaaaataa
    11281 aagctgaaag gaataaggta taatgagaaa gattaaatta ctataaatca actcattata
    11341 tcttattctc ttccgctcct acatcttatc aactttcaca gcttctattg ctaatccggt
    11401 ttctttgatg aagctttcaa tatccatcaa catgtccggg tcattgcttt tgtaaaacca
    11461 aacaactttc acctctccac cttcactttg atattgtttt aagataagta gtatttccaa
    11521 aatcatgcga gacgaactgg tattgaaata agttagtttt actgtaaaaa caaccggttt
    11581 tttttcagct atataagttt tgagccagtt tattacagga agataaaatt tataagtctc
    11641 ttccatgtat gattcgcccg atattacaca ttctccagta agataattaa aatctactat
    11701 tggataataa accaactctg gataaatatt taaataaagg ttttccatca tgcacaattt
    11761 taaaattaac tttttgatat aaatgcagaa atattgaaag aaatatcatc atcagaagat
    11821 ttcaggaaat catacttcaa ttgatttttt gaatacaatt ttatagaaat caggccaatg
    11881 tgagcccctg tagcaactaa tgaagactga tgcctcaatg ttgatttcag gttgataagc
    11941 tcatcagcat tagacttgtt taaattttca caatacttac aaagtttctc tcgatgctca
    12001 ggttgaatgg cattagaagt ggaacaaaca tagccatctt ctttttcttc aacaacaaat
    12061 ctgcctatac ccaaggtgtt attattgctt tgtctaaacc tttctgcaga atataatgca
    12121 acgttctggc atatctcaat aaaaaccctg taaagactta tccgggtttc ataagcatct
    12181 gaaaggtttt cgataaaata tctgccagaa gaggaaataa ttctcatatt gaatgggccc
    12241 tcaaaagaca gataaaaccg tttgtctttt tgttctttac tcattttatt tcagttttaa
    12301 taacaaaaac cgtaatgtca tctctttgtt cctcatcttg tttgaattcc ttaaaactat
    12361 cgtgaagttt acccaacaaa ttgcttgccg ggtcttttat attcgcctta ataaagtcca
    12421 aaaaccgttc gctgccataa cgggccctaa acacattagg ttggtcaatc atcccgtcgc
    12481 tataaaggat taacgagtct cccactctga ttgttttatg gatttcagta aactttttgg
    12541 ccgagccctg gataccgcca atagtgataa aatcaccttc aatcaaaaca gcatcaccgg
    12601 cagcagtaac ccaataaagc ggtcgtttag cccccgaaaa aacaagttcg taatgctctt
    12661 tgtcaatagc tgcaagacgg cacaggcaaa tgtccattcc atccatattt tggctaatat
    12721 cttgatgaag ctctttgtgc aacatctgat ttatcatctg caaagcctga cttggagaat
    12781 atactttacg tgtggctaca atttcattga gcaacctgtt gccaatcata gacataaaag
    12841 cccctggaac gccatgcccg gtacaatcaa caacggcaaa aaaatgataa ttaaccttaa
    12901 cttcatttgc attgtaatac caataaaaat cgccggatac aatatcttta ggcaaataca
    12961 atacatccat atcaaaatcg acattcaaca aattgggcag cattgcctgt tgtatgcgca
    13021 aagcatactc tatactatca gttattttct tgttttgcaa atcaagttta ttccgctggg
    13081 ccgtaatctc ctctttttgt aataataaaa tcttattatc tcgaattttt ctaaaaataa
    13141 aaaacaccaa tgctacaaca aggacaagcg caattgtaat tcccaaaacg agcattagtc
    13201 tgatttgctt ttcaagcttt agctggtact ctttttcctt aattactgca tttcgttttt
    13261 caacctcaaa ttggctttgc atcgccattt tcatagcaac aaaaagtgaa tcctgcacta
    13321 ctttcagctc tccttttttc actttcagtt cgagagcggt tacatgtttt tcaagattag
    13381 ccttagcaat ttcttcttcg cttttagatt tgatttcatc aatctcctgg gctttcatct
    13441 ttttgtctat caacgaataa gcctcgtaat acttgaaagc ttcagaggaa ttcccaagtt
    13501 tttcattagc ctctgctaag agggcattac aacgggctat agaacgcagc atcgacaatt
    13561 cattggcaat agcaagcgcc tcctttgcat tttcaacagc ttcattatag cgctgttctt
    13621 caatatttga attactaata tttatcaaag aattcatcac ttcgtttttg ttgcccattt
    13681 tgcgtgcaaa acgcaacgac tccataaaag ctttcctggc ttcgccatgc ctgttatctc
    13741 cactatatac aaagcccaaa ttattggcaa cagtaataca agctacattg tttcctgtca
    13801 gaatgtataa atcaagagct ttgcgaaata caacaaccgc tttatcagca tgaccgctgt
    13861 tccagtatga gtagccggct ttattaaagt aagcagcagc ctgaatagtg tttccctgtt
    13921 gttcataccc acgggcaagg ttttcaaggt catgcgcatc ttggcctttt gcagagcccc
    13981 agatgaccat aacaaccaaa acaacaagat atttagggat tttcaattgc atttctcacc
    14041 gttaatctaa taaagttagc aaaggatttt cttataaaaa attatttaaa agaaaaaaac
    14101 attatcagcc tccaaaatga cgcaaccatt atttaaatct gtatctaaca ttcaaaaagg
    14161 caaataccga taagatatct gtaactgcaa agtagttaaa tgacagctat aacatcggtt
    14221 ttaaaaaaca ataaacgaaa gggcaacttt gttataactt tgaattgaaa atactataag
    14281 cgcagtttga acaaagagtt ggaagatatc atcaaagctt gcatggccgg tgacaaaaat
    14341 gggcaggaag cattatacaa gctatttagc ggtaggatgt gggcggtatg cttgcgctat
    14401 gcaccagaca gggatgtagc aaaagatgtt tttcaggatg cattcatcaa ggtctttgaa
    14461 aaaattaggc agtttcagtt taaagggaat tttgaaggct ggatgcgtaa ggtttttgtc
    14521 aacactgcca ttgcttgcta caggaataaa aacacgttaa catacgaact gagcccaaac
    14581 ctgctgcctg aagagaatga tgacagagaa gaactcacag actcggacat ttcgagagat
    14641 gagctgatga aaatgattga agagctcacc cctcagtata agatggtttt taatttgtat
    14701 gctattgaag ggttttcgca caaagagata gcatctatgc tttctatttc tgagggtacg
    14761 tcaaaatcaa atttatcaag agcaagagaa atactgaaaa aaaagatttt aacacgattg
    14821 ggaaccaata tttacgcagg ataatatgtc tgaaaacaat atagatagga tttttaaaga
    14881 aaagctgtat gaccatgcag cccttccccc atcggatagc tggtcaaaca tcgaagatgc
    14941 tttgcacaac cacaaaaaaa gcaggtttat ctctatatat tgggctatgg ccgcatctct
    15001 tgctttgtta gttggcatcg gaaccggatt ttatattggc ataaaccatc agaaagatat
    15061 cggcaactct attgccagta ataacaaaac taatacaata aaaaaaatta aaactaatga
    15121 aataaaaacc gtttcaaata atcaaactat tgaaaaccca gttatttcag aaaaaaacgc
    15181 aaataataaa tcaataacat ttacaaattc aaaaaataca gccgataaca tattagaaga
    15241 aaagactaat attgaaacta caactgtcat tactaaaaat accgaggtag aaaaagacgt
    15301 atatgagcaa ggacaaacta cactccttgc ttatcaagaa agtaaaagca taactaacct
    15361 taaaaatact ttaaattatg agctatgtct taacacgctc tacgctatgg aagaaaaaga
    15421 aagaccagaa actgaaaacg tatctaaaac tcaatatttt attggtgtga actggactcc
    15481 tctttatgca taccgtaata ttggctacaa caacacaagc atatcaacaa caacatatga
    15541 caaatcagaa aaaccaatat attcattttc aggagggctc tcatttggta taatacacaa
    15601 cagatggtac tttcaaacag gcatatcata cacacaaatg gggcaacaaa tccccaatgc
    15661 ctctgtcccc agcacaaaaa gaaacacata tttagcagga gatgatgcat cttggacttc
    15721 ctctgtttca attcctaaat atgaagctac agctaattct atggggaact ttgaagcaaa
    15781 agaacttgaa aattcagagg cttatgcaaa tgtaaaatca gaagcaactt atagaagcga
    15841 gtcacaggta agcactgtca aatatctcat caattattca ttcattgaag tgccaatatt
    15901 ggcaagttat aaagtgtcca cacgactaat tgactttcgt tttacaggag gagcaagtct
    15961 aaactttgtc attaacaaca gagttaagct atttaatgga agtattaaag aaaacatagg
    16021 aacaaactcc aacgccgctc catttaatct tgcaggcatt atcgaatggg gcattgacct
    16081 ccctatgact tcaaaattac attttgttgt tgggcctcag ctcaggtact tcctcaactc
    16141 aattaacaaa agcaccaaca taaaaacata tccctacagt ataggaataa atgcaacgtt
    16201 gcaatatcac ctgtaaagga aacgcctgat attttgggca ataccctcaa tgagcttagt
    16261 acgggcttcg atacttgtcc aggcacaatg cggagagaaa cttattttat ctttattaaa
    16321 aacatgcaac aaaggattat cagcttttac aggctcaacc gaaaatacat cagttgcagc
    16381 gccagctatt atcttattat caatggcaaa agccaagtct ttttcgacaa caatacctcc
    16441 acgagctaca ttaatcaaga tggcagaagg tttcattagc ttaagtttat caatatcaat
    16501 taaattaatg gtattgttat ttaaaggagc atgaatagta ataatatcag aaattataag
    16561 aagttcatca agagaaacct ttttataatc agggttatta ttatttccag atgttgagta
    16621 atagcaaaca ttagccccaa aagctttagc aataagagca acctttctgc ctattgcccc
    16681 taagcctatg ataccaaatg tcttcccata tatttcgcct atttccatat ccatgtgcga
    16741 aaacatgtcg tatttactat acgaaccgtc tttcacaaat tggtcataat atcgaacatg
    16801 gttgagcaag gaaagtatat gggcaaatgt agtctgagca acactgtttg tagaatagtc
    16861 tgagacattg cacacctcaa tcccttttga aatggcatat ttcgagtcga tatgatttat
    16921 tcctgttgct gctgcacaaa taagcttcaa tgaaggggat gcatctatta ctttttggtc
    16981 aattaccact ttacaggtaa tgacaatatc agcatctgca gtacgttgag ctgtcaactc
    17041 agcaggagtg agcggataat aaactacctc gccaaattgt tcaagaagat aaagattgga
    17101 cacattgccc atggtttttg tgtccaaaaa aactattttc atagcttaat gcagctttgt
    17161 tccaataagg tgaataaatt cttcgcgggt tttagtatca ttgagaaatg cacctgtaaa
    17221 tgcagaagta gttgtaacag agttttgctt ctgcacaccg cgcatcatca tacaaaggtg
    17281 cttagcttca ataactacag ctacccccaa aggacgtaag ccttcatcaa tacaatcgcg
    17341 gatttgcata gtgaggcgtt cctgcacctg caaacgacga gaataagcat caacaacacg
    17401 ggcaatttta ctaagcccgg ttatcttgcc attagggata taggcaacat gggctttacc
    17461 aaagaaaggt atcatatgat gctcgcacat cgaataaagc tcaatatctt taactataac
    17521 catttgctta taatcctcaa taaaagtagc cgattgtaaa atctttctag ggtcaatatt
    17581 atatccctga gtttgaaatt gcatggcttt tgctacccga ataggggttt tctcaagccc
    17641 ttcgcgtgtt ggctcaggat caataacgtc taatacccct ttataaagct cttcaagttt
    17701 ttgagtacgg gcagcactga attgttcaat tttggcatac tcatcatcat tgatagtacc
    17761 ttcgcccata atctgtataa aatcattatc cataatattc aacggtgtta ttctcagttt
    17821 cttcaagtct tatacaatgc agctcaatac ctaaggctgc tattttatca taaagctcat
    17881 tccatattgc aatagcaagg ttttcagcgg atactatttt acctttcatg aagtcaacct
    17941 ctaaattaat gtttttatgg tcgagttttt caattatttc atttctaatc agttggctta
    18001 agtggctaag atttacaaca catccagtct cttggctggg caaccctttt acagtaacat
    18061 ataaaacata attatgaccg tgccacaaag gattggagca tttgccaaaa acctcaagat
    18121 ttttcgtatc atcccactca ggcctgaaca tgcgatgtgc agcattaaat ctctcacggc
    18181 gcgtcaaata taccagactc attcagtttg ttatttttta tcaaaacttt cttcgttaaa
    18241 ctccgaaagt ttttttattt tgtatatcga tagtttttta caaaaaatct attatccaaa
    18301 attagtattt tattttttag cagctgaaag attttggttt ttgaagatat tttacattca
    18361 atttaacatt aaaatttcca tattagttaa acaaggagga tgaagaaaat acttattatt
    18421 tctgccacag aaaaagagat aaaccttctt aaacagaatt tgcaaggcct gacccaggtt
    18481 aatgcatgga ctttttctgg caaattcaat atgctcgaca tcgacatcct cattggaggt
    18541 gttggcatcc cggccaatgt ttacaggctt accaaaagac tctccgaaca acattatgac
    18601 ttggtaatca atatgggcat tgccggttct ttttcagaaa agataagaaa tggcgatgtg
    18661 gtaaacgtta caaaggaata ttttgctgac attggcattg acgacaacgg tcaatttaag
    18721 aatatatttg aaatgggatt tcttgacaaa aacacgccac cgtttgtcaa tggagcgcta
    18781 aataacccat ttaccttcag aaaatttgaa atattttcaa aaatacctaa tgttacaggc
    18841 atcactgtaa acactacttc tggaagtgaa ggctgtatag tcaacaggtg gattaaatat
    18901 cagccagatg ttgaatcaat ggaaggtgca gctgtatttt acgtctgtct gcaagcaaaa
    18961 acgccattta ttgaacttcg ttcgatatct aacaaagtag aaccgcgcga tgtcaagaaa
    19021 tgggacgttc ctcaagccat catctcactt tcaaaccatg taatgttttt actctccgaa
    19081 ataaattcca aagaatgaac ctcagcatag cttattcccc ctgccccaac gacacctata
    19141 tatttgatgc ccttgcccac aggaagattt caatggaagc ccttagtttt gaatgctttt
    19201 tggcagatgt agaagagctt aatcgtgctg cacaaattgc tacttatgat atttcaaaaa
    19261 ttagcattgc cgcatacccc tctattgcta acaaatatca aatacttaat agtggaagcg
    19321 ctttaggcag aaataatggg ccattactta tcagcaaaaa gccttttaca acaaaagata
    19381 taaattcact tcacattgca atacccggca aacataccac tgctaacatg ctcctctcaa
    19441 tagcctttcc aaatgctaag aacaaaacgc caatgttatt ttccgacatt gagaatgcgg
    19501 ttttaaaagg tgaggttgat gcagggctta taatacatga aagtcgcttt acatatcagg
    19561 ataaaggact aaaaaaagtg gtcgatttgg gcgaatactg gcagaaaaca tttgatatgc
    19621 ctgttccctt gggctgtatt gttgtaaaac gcagccttaa caacgaaata aagaagagca
    19681 ttgacaatat tatatataga agccttgatt ttgctaacaa aaacaaacaa tcttcgcaag
    19741 catatataaa agaacatgca caaaacatgg gagatgacac cattgcaaaa catataggcc
    19801 tgtatgtaaa cgatttttcg ctttcattag gcccgaaagg caaagatgct atacttttta
    19861 tgcttaaaga aggtgaaaag gcaggccttt tgagcttaat cagagatgac atttttatag
    19921 actaaggttc tacccagtct ccgtgctccc gaataaggtc gattaactga tctaccgctt
    19981 cttccgaagg tatattttta cgaaccacct cttttccttt ataaagagta acctttccgg
    20041 cagctgcccc tacgtaacca tagtctgcat cggccatttc gcctgggcca ttaacaatac
    20101 aacccatgat gccaattttc agatgcctca gatggcttgt acgctgctta atacgggctg
    20161 tagtagcttg caggtcaaac atagtccgcc cgcacgaagg acaagagata tattcagttt
    20221 tgctcatcct caacctggtt gcctgcaata taccaagaga caatgcacat atagactttt
    20281 gaacactttc agcagcattt ctaatccata tcccgtcacc caacccatca ataagcaaca
    20341 acccgacatc agcagcagct tttatctgaa acatctcaag gttatattca caatagttgc
    20401 ggttgatgat taccggatta gtcaaagatg ccgccaacat tttatacaac aacgctcgtt
    20461 gttcagcaac cccgttagta ttaaatgttt caacaataac aaccgttctt cgatcatctt
    20521 tcaaagcttc aatcattcgt tcatccatct cagacaacga caacacaaca aaattgagat
    20581 gcggatgttt ggggccatag aataaatact gggcggcggt ataaaaagga aaaacatttt
    20641 tcttttcacg aaaatattta aaccagtcgt tcatgctggt tatatagcgt ttattgtctt
    20701 tcaggacaat actttcagct cttgtagcaa agaaaacata ttcagcaggc agatgcgcag
    20761 cattggtaat atctgactgg taatcaacaa taactatggg agtattattt cctccaacat
    20821 tctcaacagc aatagactcg cgctttttat attcaaaagg atttttttgt tcttcacaaa
    20881 caacatcaat agccggattg gtttttcgac cagaaaaata atctaccaga tttttagcca
    20941 ccggaatctc caactcaggc acttctgtga gggaaaccct aacggtatct cccaatccat
    21001 ctgctaaaag agcacctata ccaacagccg acttaatgcg gccgtcttca ccatcgccag
    21061 cttctgttac gccaaggtgt aaaggataat tcatatcttc agccatcatc gtagccacga
    21121 gcaagcggta tgcctgcacc ataatacgtg tattactagc ttttaaagaa actacaagct
    21181 gatcaaagcc ctcattctta caaatacgaa gaaactccat cgcagcctcg accattccca
    21241 ttggagtatc gccatatcgg ctcataatac ggtcgctcaa cgagccatga ttgaccccga
    21301 tacgtatagc tgtcccatgc tctttacaaa tgcgtagcag agggagtaaa cgttcgcgta
    21361 tccgctccaa ttctaactta tattctacgt cagtatattc caataattca aaccgcttct
    21421 tatcggcata attaccggga ttgatacgca ctttctctac aattgcagca gctacttcag
    21481 cagcctgagg gttaaaatga atatcagcaa taagcggagt tttgtaaccc cgtttgcgaa
    21541 gggatgattt tatagcagcc aagttctggg catcctgcac acctggagca gtaattcgca
    21601 cataatccgc cccggcattg acaatcctga tacactgttc aacagttgca tttgtatcaa
    21661 gggtaggagt attagtcata ctctgaaccc taataggatt agagcctccc atacaaactc
    21721 cacctacatt tacctctttg gtaacaaaac gactaaattc ggttaaagaa ttacagtatt
    21781 taaaagacaa actcatatat ttttaatcaa cgattcaaca tactcctcac tcagccatcc
    21841 ttcagccact tgctgaacct cgccacaaag gcggatattc caactctcat ctaccgaaat
    21901 atgcaactgt ccgcctaaca tatttacatc tatttctttg cctacaaggc cttttttgtg
    21961 catcatggtt gcagctgcac ttgacgaact gccagaagcc aatgtaaaac cagctccgcg
    22021 ttcccagata agcatctgaa tagctttatc tgataacact ttaacaaact gaatgtttat
    22081 tcggttgggg aacatcggat gattctcgat tttcggccca aattccttta ccaattgttc
    22141 atccagggta tcagtaacaa tgacacagtg cggatttccc accgaaatac agtttatttt
    22201 aaagattttc ccatcaatag taagaggaaa gtcaatacac tcaggcaagt cacaattaac
    22261 aggtatctgc ttaggctcaa aaatggcttt cccaatgtct atggtaatga tttcggcttt
    22321 accattgcgc tctttgttaa ttgtagcttc aacaatacct gccatagtct cgattgtaaa
    22381 atgacgtttg caggtatagc cataatcaaa tatgtactta gcaaatatac gtaaaccatt
    22441 gccactcttt tcggcctcgg agccatcagg attaaaaacc ctgaacttaa aatcagcaac
    22501 agaagaagac tctaccaaca aaataccatc agacccaata ccaaaatgga tattacaaat
    22561 tttctcaata ttcggggtat taatgtcaaa agtgatattg ctacagtcga agacaatgta
    22621 ttcattgcct aaaccgtgcg attttacaaa aaaattcttt ttgtccatgt ccgtgtattt
    22681 taattgggaa acacaggtaa aagtatggat ttttcgattg aagagaaatg aggtttggaa
    22741 aatttttaca aaatacttgt taaataaaaa cgcttaaaaa atgcaaatta acctctattt
    22801 atcgcagaaa tatactattt taggacactt ttttaacata ccattatgca actatcggtt
    22861 gttatagtaa attacaatgt aaaatacttc ctggagcaat gcctgcattc tgtttataaa
    22921 gcaattaagg gtattgatgc ggaagttttt gttgtggaca acaactctat agatggctca
    22981 tgctccatgg tgaaagagaa attcccctgg gtaaatctta tctgcaacaa caaaaataca
    23041 gggttctcgt acgcaaacaa ccaagccata aggatttcaa aaggcaaata cgtcctcttg
    23101 ctcaatcctg ataccgttgt gcaggaagat accttcagca agtgcattgc ttttatggaa
    23161 aacaaccccc aggcaggggc aattggcgta aaaatgattg atggcaaagg aaatttcctg
    23221 ccagaatcaa aaagggcttt acctactccg gaagttgctt tttataaaat atttggcttg
    23281 gccaggctat tcccccaaaa cagacgtttt ggacaatacc atttgtcata tcttgacaag
    23341 gacaaaacaa atgaagcaga cattttatgt ggggctttta tgtttatgcg caaggaagct
    23401 cttgacaaat caggactttt agacgactca tttttcatgt atggtgaaga tatagacctt
    23461 tcatatcgta ttatcaaagc aggttataaa aactattatt ttgccgatac caccatcata
    23521 cactacaaag gggaaagcac caaaaaaggg agcctgaact atgtactggt gttttaccag
    23581 gccatgatca tctttgcaaa aaaacacttt tcaaacaggc gctttaagat gtttaacctg
    23641 cttatccacc ttgccatata ttttagggca ttattatcca ttgtaaaacg tgtagctaaa
    23701 gcgctgtatt tgcctttgat tgatgctgtt tctatttatg cgggttatct gtttgtcata
    23761 cgcccttttt cagaatcaat gaagtacgaa cagggaggcc attacccatt cctttacata
    23821 gggctcattg tacctttgta cattttaatt tggcttatat caatgatctt agcaggaacc
    23881 tatgacaaac caataaaaat tacaaacatt ttcaaaggca taggcattgg gaccctgatt
    23941 attctggcta tttacggact actccctctc gactaccgat tctcgaggct aatgataata
    24001 cttggcgctg tatggagcct tggcactatt agttttatcc gcactatatt acatttgaca
    24061 gggttcaaag actttaggtt atacaacact aacaaaaaga agataatcat tgtaggtaag
    24121 tatgaagagt atctaagagt aaggcaactc ctcctacaaa cctcgttaat ccatgacata
    24181 acagggttta tagatgaaac taacaacgaa tcaccagatt atcttggcaa ttcatcacag
    24241 cttaaagata ttatttatat ttataaagcc gatgagataa tcttttgtgc caaaaaccta
    24301 tcatcacagg acattataaa ttacatgctt aatttttcgg catctggtgt cgagtttaag
    24361 attgccccac cagaaagcct ttcaattatt ggaagtaact caattaacac tgccggggac
    24421 ctttatctaa tcaatatcaa tgcagtaaat aggcctgaaa acctacgttc aaaaaggctt
    24481 ttcgattttt ctatttctct ttgcctttta ttaacataca ttttcatttt tccttttgta
    24541 aaaaacaaaa tcaaaggcct ttacaacatt cttcttgtgc ttgcaggctc ccggacatgg
    24601 ataggttaca cttcggatga aacccaaaac aggttttcgc tgccaaaact aaagaaaggc
    24661 atattaagcc ctgtggacgg attgctcaaa aaagacttat cttcagaaac aatagaaaag
    24721 attaactttt tgtatgccaa agattataaa gttatcaatg acttcaacat cttgtggcac
    24781 ggacttgcta atataggcag ataagtatga tagcccttta aaaactgcaa tctactcttc
    24841 aaatacctgt atttcaaaag taattttcat tgccttttta tacgcagccg aaactccaca
    24901 atagcgttcc tgcgacaaat caacagcttt ctgaagcttt tctttatcca ggcccttgcc
    24961 ccaaaactta taaataatgt gcattgctgc gtaatgcttc ggcatttctt ctgtaagttc
    25021 accttcgaca gcaatatcaa acttttcagg ctcaacacgc atttttttga ggattgatat
    25081 cacatccata ccagtgcacc cagcgagaga agccagcatc aactctttag gccttgagcc
    25141 ttcatcattc cctccatgtt cgggagttgc gtccatcttc acatgatgcc cgctaactaa
    25201 ggcatcaaaa agcatattac ctttccaggt tgtattgact atgtgtttca tgctttttct
    25261 tttttacgaa tataacaatt gtattgcttt tttgttttgg aacaaaaaag ctgtgcttat
    25321 gctttacagc ataaaacaca gccccctttt aaaatactga atctcttatg gcctaaaagc
    25381 tgtcagtagc gaataattga cctttttacc tttattatcg taattctcag tctttacgac
    25441 accaacatcc tcagcaaccc attgaacagc cttaacttcc acatcaaaca tggcatgagt
    25501 tctaacgttg taggtaactt tatagcaatt gaatgtaccg gcaggagtag ttatttcttc
    25561 gtacgactca actttacggt tggtaataga catcgacatt tccattactg taacgttacc
    25621 acttttcatc accatggtca ttttggcatc tttcagtgtt tgacctatct gaggatttgc
    25681 cggaaactca agttcgtcag actttacttc catgcttgcc tgatcgccat tttgctgggg
    25741 catcatactt tccatgttaa agtaaaaaac accattgtgg cacctgacct tgtattgtcc
    25801 tttgcctttt tcttgccccg aattgtctga atataccgac tcaaacagca gagaaacacc
    25861 attggcaccg gtttctttct tcaatatcac ggttttggta acaccttcaa ttttatcttt
    25921 cgcattgtac gatgtcattt ccgactccga tccttcccgt gaaggaaaat acatgttaca
    25981 tttctgggca ttgatcccta ttgatgcaca gaacaacact gtacccatca atactaattt
    26041 tttcatttcc atagttttta ttggtttaag attgaataaa agtaaacaaa agaaattaca
    26101 ttctcacttc aaaaaagcca ttacaaacca aaagcattta taaaaagaaa aaatcccctt
    26161 tttggggtat attctgaaca cctttcataa cctaatattg cattgaaatc aaaaagaaac
    26221 cttaatccaa attaataacc tttttgtttt acctgcatgt aaaagtttta ttatgtgttt
    26281 gaaaatcatt ttcattgtta ttttacatgc aggattttaa aaacaatgaa aagattgtaa
    26341 aacaaaataa aacctaatga ttatgaaaaa aacattactt tttttactga gttgcctttt
    26401 tttagtatta atgagctgct caaaagatga cgacaaagac agtgaagatg gcacaacaga
    26461 caccccacaa agtacagacg ggaaagtgac ttttttgaaa aaatacgaag taaagtcggg
    26521 tatcatcaaa tttaccgaat attccgacat attagatgaa accactacct acaaggtatt
    26581 ttttgacgaa tacggtggca aagaagtaaa atatgaatac gatgccaaag gagctcttac
    26641 ggacattacc atggaaaaag gagatggctg gtactaccag ataagtatta agaatggagg
    26701 aacgaaaaca tcttctcctt atgcagatgg tacagaaatg aagtttgacc ttaactggac
    26761 cgaaaaacaa aaaaccgaat acaacttcaa aatgctcagc gacactacca tagccggaaa
    26821 agtctgcaaa aggtattcta tggatgcgtc agaatctatc agcggaattt atgccggata
    26881 taaaggcgtt accctttatc agaaatctat atctaaaggc tcatttactt tttatgtaga
    26941 agccaaagcc gtttcgtttg aagaaaacgt agccatcaac gacacagtat ggaaactacc
    27001 cgccaatatt accatcaccg aatcaaaata ttaaaagctc atttacacaa tgtttcccgt
    27061 gtagcataag ctatgcggga atttttgtat atatttcttt cttctcttaa aaaaaacaaa
    27121 gcaagtacct atattattta ctttcagcat cttaacctaa tttgcataaa gcagaatact
    27181 tttgtatctt taaaatttga aaaaatctaa attaaccata aaatattaaa acatgaaaac
    27241 acattctact ttaagaaacc tggcaataaa agcgataatg ctggctctgc tatgggtacc
    27301 cggcaccttg tttgcccaga ttgtttatat atcgcccagc gggagtgacc ttacaggaaa
    27361 cggaagctat gatatgcctt ttaaaaccct tcaaaaagcc ttagacaatt gtaacaacaa
    27421 tatcatctct tggaatattt atttatttga tggtacttat gatttcgaca caactaccat
    27481 ttccattgcc tcacaaagta ttaacagtat ttctgctatc aatacaaatg gagcgactat
    27541 taaaaactct aaactgattt ttgattttgc ccaaaccaat caatatactt cattcattaa
    27601 cctggtgatt gattcttcat ctatagaatt ttcaggagca ggaactgttt atatttctga
    27661 taaccacttt aagaatagca cttatatctt agcaaaaaac aatactataa ctgctgatgt
    27721 attgaatagt atttttgaaa attctggaat ggaattaaat tcattgtcag ccacaattat
    27781 taacaacacc tttgtacgtt ccttatcagt tacaggttct tatgccattt atgtccctca
    27841 ggctgtaaac atctcaattg aagggaatat tttcaaaggt tacgactatg caattgacgg
    27901 aactgcccaa aatgtgaaat acaattgctt tttcaacaat gtaactaatt atccttcaag
    27961 tcttgattcc agcaatatat actcagaccc catgttaaac agtgacttca gtctaaaacc
    28021 aggctctcca tgtattggag cttatgaagg ctctgacatt ggggctatta cttatgtaac
    28081 acctgtatgt aattttgatt ttacttatgt caacaattat ggagccaact acaccttcac
    28141 tccacaacct accaactgta atacattcaa ctcgctaaca tggacagtaa gagaccctaa
    28201 caatatcctt ataaaaacca gtacagacaa tattttcact tacactatgc ccgaaatagg
    28261 ttactataca ataacacttg aaggcttaac agtaaatagt gaaacatata cgaccagcca
    28321 atatgtaaat gtttataata cctgtccggc aaatggttca atgaatatca tttctgccga
    28381 tgaaataact ggcctggtta agtttaaaat ttccgccatc aataccgact cggtattttt
    28441 ctcctttggc gacctaaaag actctgtatt taaatattca gggactgaaa tgactttcac
    28501 acataaatat ccaaccaatt ggaaatatgg aaatgaattt tacccaagtt attcattata
    28561 taacaccctt gaaaactgtt cgatacccaa atattctgat atccctgtaa gtattaaaaa
    28621 taaaaattgc aggatcacca cttgggctta ttcagacact ttaacacttt cagtaaacgc
    28681 tgaaaaatat ggcaatgaag acgttttggt aaccctgaaa aactcaaaag gcactttaga
    28741 caattttaca cttaacacta g
//
LOCUS       DAOSPF010000046        27680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1735_length_27680_cov_7.758842, whole genome shotgun sequence.
ACCESSION   DAOSPF010000046 DAOSPF010000000
VERSION     DAOSPF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_27680_cov_7.758842"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1116)
                     /locus_tag="PK252_09770"
     CDS             complement(238..1116)
                     /locus_tag="PK252_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOT15035.1"
                     /translation="MNTTTLPNNIISIIKGFQQNELTEAVIYERLARIEKKDENKKIL
                     EKIASEERSHHDFWRRYTNTDVEIKGFKVFFFFWVARIFGLTFGIKLMEKGESNAQNL
                     YSQILPHVPEAQALINDEAEHENQLIALIEEEKLNYVGSIVLGLNDALVELTGALAGL
                     SFALQNTKVIAFAGLITGIAASFSMAAAEYLSQKHEGVENPLKSATYTGIAYIATVGL
                     LITPYLIFSHYLVCLFVTLSVALFIILIFNYYVSVAKDLPFKKRFAEMAIISLSVSAI
                     TFGISALVKTYFGIEI"
     gene            1188..2840
                     /locus_tag="PK252_09775"
     CDS             1188..2840
                     /locus_tag="PK252_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT15036.1"
                     /translation="MSSKSYSFGMGTKPLLGETIGENLRRIVQTYPDNEALVVVHQNY
                     RATYKEFWDQTTQVAKAILAAGVVKGDRVGIWAPNRYEWVLVQYATARIGTIMVNINP
                     ANKLADLEYVLNQSKINLLIASKGFRQSDYVSILHQARLHSEYPKRSVIMERDWDKFI
                     ESGQKITDQQLEEIEKSVQFDEPVNIQYTSGTTGFPKGATLSHNNILNNGFFIGERLY
                     YTEKDRVCIPVPFYHCFGMVLANLAITTHGACMVVPSESFEPEIVLKTVEAEKCTSLY
                     GVPTMFIAELEHPNFSKYNYSTLRTGIMAGSPCPVETMKQVQSKMNMTQVAISYGMTE
                     TSPVSTMTFADDRLDKRVSTVGTPLPYIEVKIIDPKTGEIVPRNTKGELCTRGYSVML
                     GYWDNEADTRLAIDAAGWMHTGDLATMDDDGYFAIVGRIKDIIIRGGENISPREIEEF
                     LIRHEAISDVHVIGVPSQKYGEEVMAWVKKKEGMELTEEELNSYCKGQISTFKIPKYW
                     KFVDSFPMTISGKIRKVEMREISAKELGLEQFNQFKGNGNNE"
     gene            3147..4379
                     /locus_tag="PK252_09780"
     CDS             3147..4379
                     /locus_tag="PK252_09780"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT15037.1"
                     /translation="MAKEKIVLAYSGGLDTSVILKWLLDKNYEIIAYIADVGQDEDFE
                     AAKEKALKIGASKVYIEDLKRELVTDYVFPAIKANAIYEGRYMLGTSLARPIIAKRQI
                     EIAAAENAAYVGHGATGKGNDQVRFELTYYALNPSIKVIAPWKMQEFLDQFKGRTDML
                     KYAEEKGIPVKATLKKPYSEDDNLIHISHEAGILEDPLHPWSADMLTKMVRPQDAPDK
                     ETKLAIYFKDGIPVKVVNKDDGTTVTDPLELLNYVNKIGGENGIGICDMVENRFIGIK
                     SRGVYETPGYTILRTAHMDIEGVAMDREVMRLRNMLAEKFAELIYNGFWYSPEMDFLL
                     AAINKSQELIDGVVYVTLYKGNVLVDGRESPSSLYNQDLSSMDIEGGFDQKDSAGFIK
                     INAVRLMAHNAIVRRQKK"
     gene            complement(4713..5309)
                     /locus_tag="PK252_09785"
     CDS             complement(4713..5309)
                     /locus_tag="PK252_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15038.1"
                     /translation="MKRIFLISAILLLTSINMLAQQYIDVVYLKNGSVIRGVIMEQVP
                     NQSIKIQTADKSIFVYNMDEVDKILKEEAKTNSSSKSSPISSQGRESYSISIEAGLLL
                     TGPGKINVGGYEMKPEISPLVKMDVDGILVPKLSMGVSAIFGLLGVDGYDESSNYMSI
                     GGTIKPIFNAGTDMQIKPGLFVGYNYINNDDREITVTG"
     gene            5720..6196
                     /locus_tag="PK252_09790"
     CDS             5720..6196
                     /locus_tag="PK252_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT15039.1"
                     /translation="MEAFESNNREILSLIEVWEPKLLALSNDVISNKRNVQNRTIKQI
                     LGHLVDSASNNTHRIIHLQYQFNPLVFPDYANLGNNDRWISIQNYQGEDWGTIVQLWK
                     YANLHLVHIIRNVNSEKLENEWISALGEKVKLKDMIIDYLRHFKLHLGEIEELINK"
     gene            complement(6302..7381)
                     /locus_tag="PK252_09795"
     CDS             complement(6302..7381)
                     /locus_tag="PK252_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15040.1"
                     /translation="MKQKMNKPIMKSTNPYRVLLRGAFIVVGGYALIYIALNLLFFKQ
                     FSFIVLISSSIIIFGLTVLVFSYVLERYIYDKIKLIYKTIHTLKLNKDQKIKTVNLKE
                     DIIAKVQEDVEKWATQRKEEIEELRKLEVYRREFLANVSHELKTPLFNIQGYVLTLLD
                     GALDDPEVNHEYLEKTDKNIERMINIVKDLEIISQLESGEIKMIYSKFDLLSLVNEVF
                     EMLEIKAKNKNIELTIAEKTRELGSITVVADKERIRQVIHNIVENSINYGNNNGRTKV
                     TVYYMDNLVLVEISDNGIGIEEKHLSRLFERFYRVDKSRSRHLGGSGLGLAIVKHIIE
                     SHNQNIHVRSAVGVGTTFSFTLEKG"
     gene            complement(7362..8060)
                     /locus_tag="PK252_09800"
     CDS             complement(7362..8060)
                     /locus_tag="PK252_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15041.1"
                     /translation="MSRTEQYKILIVDDEEDIIEFVSYNLKKEGYKVLKAYNGAEAVA
                     IAREQIPHLIILDVMMPGMDGIETCREIRQISSLSSTIITFFTARNEDYSLVAGLDAG
                     ADDYIAKPIKPRVLVSKVNALLRRYSDNSKPARPMITLGNVTIDTERYVVIINENEIT
                     LPRKEFELLHLLASKPNKVFTRDEIYNRIWTGDVIVGDRTIDVHIRKIREKLNNDCIK
                     TIKGVGYKYETEDE"
     gene            complement(8073..8762)
                     /gene="phoU"
                     /locus_tag="PK252_09805"
     CDS             complement(8073..8762)
                     /gene="phoU"
                     /locus_tag="PK252_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT15042.1"
                     /translation="MTHLDTELQQLRASLNDMWGLTISQLHRAQKAFENNDKELAHEV
                     IANEKRLDAFELKICMDCENIMALFNPVANDLRFVLSVLRINYSLERIGDYARGIAKL
                     VIKAEQEFSKVCTERIQFDRMFTLATNMVMATGEAFEDEKNHSVRAIFHEDNELDKIN
                     KKSVDVIAELIKEYPEQTINLLNLLLAIKKLERIGDLSKDIAEEIVFYREAVVLRHKK
                     KKEKEKLTNNS"
     gene            complement(8936..9700)
                     /gene="pstB"
                     /locus_tag="PK252_09810"
     CDS             complement(8936..9700)
                     /gene="pstB"
                     /locus_tag="PK252_09810"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT15043.1"
                     /translation="MSKDKKEIEVQDLNLFYDDFHALKNINFHAKKNTVTAFIGPSGC
                     GKSTLLRTFNRMNDLIDDVRMTGKVLVEGEDIYKKGIIVDDLRKKVGMVFQKPNPFPK
                     SIFENVAYGLRVNGINDKDKIEHTVVNSLKQAALWDEVKDKLKQSAMALSGGQQQRLC
                     IARTLAIKPSIILMDEPASALDPISTGKIEELIYELKNDYTIVIVTHNMQQAGRVSDY
                     TAFFYLGELIEWDETKKIFTNPANERTQNYITGRFG"
     gene            complement(9831..10694)
                     /gene="pstA"
                     /locus_tag="PK252_09815"
     CDS             complement(9831..10694)
                     /gene="pstA"
                     /locus_tag="PK252_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT15044.1"
                     /translation="MIKKKQIEQNIAFTILRIVSFMVVAILFVILGFIIFRGAKAINW
                     TFLTAMPENGMTEGGILPAIVGTFCLIIGSAIFAFPIGVMSGIYIHEYMKDSPMKRFF
                     RNMTDNLAGIPSIVFGLFGMALFVNTLKFGDSIIAGSLTLGLMVLPVIIRTTEEALKS
                     VDDSLRTGSYALGASKLQTIRKVVLPIAFPNIITGLILSISRVSGETAPILFTVAAYF
                     LPKLPTSIFDQVMALPYHLYVITTSGTNIELSQKMAYGTALVLISIVLIFNLIAALIR
                     RYFNRKINIRK"
     gene            complement(10698..11891)
                     /gene="pstC"
                     /locus_tag="PK252_09820"
     CDS             complement(10698..11891)
                     /gene="pstC"
                     /locus_tag="PK252_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT15045.1"
                     /translation="MKLRRKFEKITESILFASSTVTTLTVLLIIIFLFKEGISLFNDK
                     PVEHGYVLSVNKENPVNILSEAQIKDIFDQEITNWKQVGGKDDSIIIFSLNDITNYYT
                     EEELGADLEFMPDKINELIDSHTGIIAYMHSSSLPKNFKGKHIEIKKIGLFDFLGGKE
                     WFPTATPAAQFGALPLILGTLWVSLGAILLALPIGLVTALYLTEVADKRVRDILKPVI
                     ELLAGIPSVVYGFFGLVVIVPIIHDVFGLSVGETALAGSIILAIMALPTIITISEDAL
                     SSTPRALKEASLALGASRWQTLYRVTIPYSKSGITAAAILGIGRAMGETMAVLMVTGN
                     ASVIPHTFLQPVRTIPATIAAELGEAPFGGTHFKALFALGIVLFIITFGFNLIVNSIK
                     ARNRR"
     gene            complement(11930..12757)
                     /locus_tag="PK252_09825"
     CDS             complement(11930..12757)
                     /locus_tag="PK252_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT15046.1"
                     /translation="MKKIILSVAVLAGLTLTTAIAQKVACKIKGSDTVLPLSQKEAET
                     FNKKTGSSVSVTGGGSGVGLSALLSGTTDIAQSSRSMKMDEKIKLKDAGKAYKEVTIA
                     LDALAVIVNPGNKVSQLTREQLEGIFTGKITNWKEVGGDDLKIIVYSRETSSGTYEFF
                     KEHVMNKKNFAPSALLMPATGAIVQSVSQTAGAIGYVGLAYVEKNVKALKVSYDKGKT
                     YVAPSVATAKDKSYPITRPLFYYYTTDVESKIKPYVDFVLSAEGQKIVLDEGYVPVK"
     gene            complement(12854..14218)
                     /locus_tag="PK252_09830"
     CDS             complement(12854..14218)
                     /locus_tag="PK252_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15047.1"
                     /translation="MKKVLFAMLGFLTVNIVQAQEEEITDSVQWLSGKIETAETEIGL
                     LKKLKFSGYIQAQWQKADTIGSPSVAGGDFKGTDNRFQVRRARLKAAYSGTNSNIAMQ
                     LDLKDDGSVKVKEAYGIYTEPLLNAFSLTGGIFNRPIGFEVEYSSSTLESPERARVLQ
                     TLFPDECDLGAKLTIQAPKTSSWNFLKLDVGLFNGNAITSETDKFKDLIAHLSFKKVL
                     MDENLQISGGISYYLGGFASPSGTLMKWNSADSTFKADAGYKKGDRIDRKYFGIDAQI
                     SLNSALGLTSLRAEYLWGQQPGTFKSTVSPKGAITDTKDYIIKDTLGNAVTYTATVKP
                     DIYLRNFNGGYVMLVHRLMQTKHEIVAKYDWYDPNTDASGDELVSKNKNVGPSDLKFT
                     TIGLGYNYYATPNVRLSVYYDMVSNEKLNAKYNKAVIAENGVKNYTSDLKDNVLTLRV
                     LYKF"
     gene            14510..14860
                     /locus_tag="PK252_09835"
     CDS             14510..14860
                     /locus_tag="PK252_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT15048.1"
                     /translation="MKRFASFIISGILCVSMLASEKPSANVKAESKVENALISGVVVD
                     KITGEPLAGVEVKMIGSNEKVFTDFDGNFQFTNIPQGAYAIKIDYISYENVVENVYAT
                     KEENPIKVKLSSVK"
     gene            14943..15422
                     /locus_tag="PK252_09840"
     CDS             14943..15422
                     /locus_tag="PK252_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOT15049.1"
                     /translation="MTKKTLFFLMVLAMPCLSVAGQDIIRVEQTKYFDKNHNLYNGAY
                     TEYYENGHKRLEMKLKNGEQDSVTVLYFENGQINETRSYKDGLMDGRWEVFDESGNKL
                     SEAFYKNNKKDGCWRIWNAKGVLLFEMHYKDGVKCGDWKTFNEDGTVKSQRSYEACL"
     gene            15440..17194
                     /locus_tag="PK252_09845"
     CDS             15440..17194
                     /locus_tag="PK252_09845"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT15050.1"
                     /translation="MELISVEDVAKAANLKGPGSKGVAKVLMSAFKLNKLNELYEKHA
                     DTKGFDFVNMILQETGIKYKVPVTDLERVPKTGPFIIISNHPYGGAEGLILLDALKNI
                     RPDIKIIANFLFQKIEPLNEFIFPVNPFETYRQVSSFQGLKEAMLHLSNEHPILIFPA
                     GEVSTFYPGSTAVADRKWSKPALKFIKKVNVPVVPVFFKGTNSHLFHLLGMIHPTLRT
                     GRLPAELLNKKDKPVNLRIGSAITVKEQAKFSSVSIYGRYLRARVYALDTSEDVKKFF
                     FPKRSESSKHEVVSAVDPLVLQQEINALPAHYKVTGQNKLEVYCAPSSAIPNMITEIG
                     RMREITFREVGEGTNMSIDLDEYDLYYHHLFIWHADDKKLVGAYRLGFGQDIIDKYGI
                     KGFYTRSLFKFKKEIYPIFTHAIEVGRSFVIKEYQKSPLPLFLLWKGIFLTLVKRPDY
                     QYLIGPVSISNDYSKVSKRILIDYITRNHFNNELAKYVKPRKKFKVRFPNVDTEILSD
                     TATDLVNIDKVIADIEHDRFKMPVLLKKYIQLNGKIVAFNIDPKFNDALDGLLILNFH
                     DTPREMIDYLSRESETKS"
     gene            17225..20086
                     /locus_tag="PK252_09850"
     CDS             17225..20086
                     /locus_tag="PK252_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT15051.1"
                     /translation="MYYKILTVIFSLFLCSYAQNTAGQASISGTVTDQKTGETLIGCN
                     VYIQGTTNGVSTDLDGNYSLKGLSAGTYTLVASYISYNTATISVTVPKNGDIKADFKL
                     EPVTVAINEVTVVARKRQNTEMSMISAIKTQDLIVNGISGQQIQKSQDRDAAEVLRRI
                     PGITITDGRFVIVRGLIERYNSVLLNGVTAPSSEADARAFSFDVMPSNLIDNILIYKT
                     PAPELPADYAGAAINVITKNTVEENSTSFSYGIGYVTNTTFKDFYTYKGGKYDWLGFD
                     DGTRALPSAFPARNVMNDSLLNFDYKPGMADSIAKISKAFRNDVFAPFTKVAKPNQSL
                     ALNINRRFKIGKMDVSNLTSLMYNYSASTQDATRAEYQDPNPITGSKEYSYLFNDMRY
                     NEAVKTGIMHNWTFAFNENHKIEFRNFLNQNSRNRTVMREGVNYYTGSLPDTIKAIAM
                     EFTQRTTYSGQLAGVSEFNDKNTQLEWILGYSYADKLQPDFKRLFFVKQEDSEGQYRF
                     RMSIANGSPLPERGGRFYLEAYEHIKNLAVNFKQNLNILQGKTTLRTGIFYEQRNREF
                     DARNIGIVFKGAYPTDMFAPIDSILQDKNFRYPGGFAYNENSPRTNHYKAVSSTAAAY
                     LGVKVPVLKRFVWYSGLRAENYEREVSEFQLDVKNVPNIKLDTLDLFFSSNLSYNINE
                     KHIIRLSYGKTVNRPEFREIVPFYYKDFDLNAGVWGNDTIENCYIHNYDLRYEWYPSP
                     SEMISLALFYKDFVNPIEVSLIETGNQPDYKPFNTASAYSRGIELDVRKSFEFLSDNT
                     GFLYYFKNFTLVFNASLIESVVKTNVPDAREKEREMMGQSPYIINGGLYFNSDKQKLM
                     LSLVYNRIGRRLAAIGTKNTPHVWELARNGLDFTLTKGLYKGIELRLGIKDILNEPIR
                     RLQYEQTNLSPTGQHVEIEQTTLEYKPGTTYSVALSYKF"
     gene            complement(20268..21014)
                     /locus_tag="PK252_09855"
     CDS             complement(20268..21014)
                     /locus_tag="PK252_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15052.1"
                     /translation="MIEIRNISKRFNGNEVLRNIWVNYEEGKTNLIIGQSGSGKTVLL
                     KTMVGIYQPDEGEVLFDGISLNTQSSAELRELRKNIGMLFQGGALFDSYTVVENIKFP
                     LDMFTQMTEKEKKERVRYCLERVNLENAGKLYPSQLSGGMQKRAAIARAIVLNPRYLF
                     CDEPNSGLDPQTATVIDQLIKDITKEFNITTILNTHDMNSVLELGEKVVFLHQGTKAW
                     EGTKDDIFNSGNELLNEFIFATTLAKHFRK"
     gene            complement(21011..21751)
                     /locus_tag="PK252_09860"
     CDS             complement(21011..21751)
                     /locus_tag="PK252_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT15053.1"
                     /translation="MGFFYHIGRYFIFLGKVFVKPEKIRIYGRQLIKEIENIGFSSIG
                     IVAIISVFIGAVLTIQTGINADTPLLPNYLIALAVRDSIILEFSSTIVALILAGKVGS
                     NIASEIGVMRMSEQIDALEIMGINSAGFIAMPKIFAAVIFNPVLTVISILVGIVGGWV
                     AGFATGVVTSEEYIYGLQYSFKPYYVVYGLIKTVVFAFLITSISAYNGYYVTGGSVEI
                     GKASTKAVVYSSIAILFFNLLLTQFLLT"
     gene            21975..23069
                     /locus_tag="PK252_09865"
     CDS             21975..23069
                     /locus_tag="PK252_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOT15054.1"
                     /translation="MGVKNNNYCIIMAGGIGSRFWPYSRTACPKQFLDILGLGSTLLQ
                     QTYNRFARIMPHENILIVTNAEYKEIVQKQIPQVPVDNILLEPARRNTAPCIAYAYSK
                     IASRTNVANIVVAPSDHLIIKEDEFLNIVQESLELVSKNDILMTLGIKPSRPETGYGY
                     IQIEKDNNKKIGNANKVKTFTEKPNYDLACMFLESGDFYWNSGIFFWSLKSISSAFEK
                     HLPELFNLFNNGHEFYGTNHEKDFIAQIYPQCESISIDYGVMEKSNNVYVMLSDFGWS
                     DLGTWGSMLENSEKDSNGNYISGENIFTYQVKKSIVKNETRKCVVVNGLEDYIVVNTE
                     DVLLICRLKDEQMIKQFVADVKMKYGEKYF"
     gene            complement(23070..23792)
                     /gene="lipB"
                     /locus_tag="PK252_09870"
     CDS             complement(23070..23792)
                     /gene="lipB"
                     /locus_tag="PK252_09870"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOT15055.1"
                     /translation="MEEKTIISFQKTDKNQEEIAFHDIGTADYMEAWQYQQKLFDQVL
                     ELKNKGSEKMLHHLIFCQHPHVYTLGKSGKEQNLLINYIQLQARNATFYKIDRGGDIT
                     YHGPGQLVGYPILSLTNLGLGLKEYIYKVEEAIILAIAAYGIKAERLQGATGVWIDTE
                     TPGKTRKICAIGVKSSHWVTMHGFALNVNTDLSYFGHINPCGFTDKSVTSMEKELNKP
                     LNFEEVEKVVLSKLLQVFGFIS"
     gene            complement(23955..24800)
                     /gene="lipA"
                     /locus_tag="PK252_09875"
     CDS             complement(23955..24800)
                     /gene="lipA"
                     /locus_tag="PK252_09875"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT15056.1"
                     /translation="MNIQRKPDWLKIKLASTADYAKVKDIVNKHKLHTICQSGSCPNM
                     GDCWSRGTATFMILGNICTRACKFCNVNTGKPLPVDWDEAARIAESIKLMKLNHAVVT
                     SVDRDDLSDFGAAYWAYTIKKIKQENPQTTLEVLVPDFQGNLQYLDKVIEAAPDIISH
                     NLETTRRLTPQIRTKAQYDTSLNVIGHIAQSGVVAKSGIMLGLGETQEEVFEVMDDLR
                     AVNCQIMTIGQYLQPASENIEVKEYIHPEQFAYYKKIGLSKGFKVVESAPLVRSSYHA
                     EKHIM"
     gene            25071..25688
                     /locus_tag="PK252_09880"
     CDS             25071..25688
                     /locus_tag="PK252_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15057.1"
                     /translation="MDKNTFDKYWAKAISYIFNPLIIPFFGIIVILYSNTYISLLPSN
                     AKYLILSIVFTGTFILPLCFVPMYMYLKVIKSIDMEDRLQRILPLAVTIILYSLTYYF
                     LIKVSIPFIGSFLLSVLFTLIILLVLLFKWKISFHTAAIGGLTALILGIMLRLNADIM
                     TWFICSIIISGLVATARLILQKHSLFEVATGYLIGFFTVFIFIII"
     gene            25695..26180
                     /locus_tag="PK252_09885"
     CDS             25695..26180
                     /locus_tag="PK252_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT15058.1"
                     /translation="MKKNILTISTLVVIIFTIVLLYPSCKVQQPVQTQDTVKIEPAVA
                     VPDTEYYAMVETPAKFNGGDIKSFIAYLTKNIKYPAVALKKRQQGTVAIQFGVDCYGK
                     VHVFSVLKSSGVAVLDKEASMAINASPAWTPALDKGRPVGQLFLLQIKFNAKTRKVEI
                     K"
     gene            26246..26998
                     /gene="gpmA"
                     /locus_tag="PK252_09890"
     CDS             26246..26998
                     /gene="gpmA"
                     /locus_tag="PK252_09890"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOT15059.1"
                     /translation="MDMYKVVLVRHGESAWNKENRFTGWTDVDLSAKGVEEAKNAGRV
                     LKAEGYTFDLAFTSTLKRAIRTLWLATDEMDLLWVPVIKSYTLNERHYGALQGLDKSE
                     TAAKHGEEQVKIWRRSYDVPPPALEETDPRHPKFDARYKDIDPKLLPATESLKETVAR
                     VVPFWEGTIVPEIKKGRKVIIAAHGNSLRALVKYLDNVSEKDILELNIPTGTPLVYEL
                     DANMKPIKHYYLGDQEAIRKAMESVANQGKAK"
     gene            27400..27528
                     /locus_tag="PK252_09895"
     CDS             27400..27528
                     /locus_tag="PK252_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15060.1"
                     /translation="MGDKTIPVARNRKKAFLKTIIRGSNTIHTEKHTTLLPKPTIQ"
BASE COUNT         8210 a         5486 c         5468 g         8516 t
ORIGIN      
        1 aatacttgat tctgagcaaa tataatcctt ttaggcaaat tatacgataa tcattaatta
       61 catttagcta aattataaat gcttaaaaat gaaggtgatt taaattactc tgtaaaatga
      121 gtttaattac cttaatgcaa atataataaa atacctataa atggtgaaat aacaaacaaa
      181 aaagctttgt ctgaattgaa agacaaagct tttaactttt aagataaatg taaaatacta
      241 tatttcaatg ccaaaataag tctttacaag agcactgata ccaaaggtga tggcagaaac
      301 agataaacta ataatggcca tttcggcaaa gcgttttttg aatggcaggt ctttggctac
      361 agaaacgtaa tagttaaata taagtatgat aaagagggcc accgaaagcg ttacaaacaa
      421 acataccaag taatgtgaaa aaataaggta tggagttatt aacaagccta ctgtagcaat
      481 gtaggctatg cctgtgtaag tagcagattt cagtgggttt tcaacgcctt catgtttttg
      541 agataagtat tcggcagcag ccatagagaa tgaggcagca atacctgtga ttaaccctgc
      601 aaaagcaata acttttgtat tttgcagtgc aaaagacaga cctgccaagg ccccggtaag
      661 ttcaaccagc gcatcattga gcccaaggac aatagaaccg acatagttta acttttcttc
      721 ttcaataagg gcaataagtt ggttttcatg ttctgcctca tcgttaataa gggcttgtgc
      781 ctcaggtaca tgtggaagta tttgagaata cagattttga gcgtttgatt cacctttttc
      841 catcagttta atcccaaatg taaggccaaa gatacgtgca acccagaaaa agaagaaaac
      901 cttaaagcct ttaatttcta catcagtatt ggtataacgt ctccaaaaat cgtgatgaga
      961 tctttcttct gatgcaattt tctcaagaat ttttttgttt tcatcttttt tttctattct
     1021 tgcaagtcgt tcatatatta cagcttcggt tagttcattt tgttgaaaac ctttaataat
     1081 gctgataatg ttgtttggca atgtagttgt gttcatatat tttttcttca aaaataaatt
     1141 aaaaagagct aaaactaaca tatatacaaa atctaaatct tgtgaaaatg tcatcaaaat
     1201 cttacagctt tggaatgggg accaagcctt tattgggcga gaccattgga gaaaatcttc
     1261 gtcgtattgt tcaaacctat ccagataatg aggctcttgt tgttgtacac cagaattata
     1321 gggctactta taaagagttt tgggaccaga ctacccaggt agccaaggca atattggctg
     1381 ctggtgttgt aaagggcgac cgggtgggta tatgggcacc caacagatat gagtgggttt
     1441 tggtacaata tgctacagcc cgtattggca ctatcatggt caatattaac ccggctaaca
     1501 aactggccga tttagaatac gtcttgaacc agtcgaaaat aaacttgcta attgcgtcaa
     1561 aggggttcag gcagagcgat tatgtttcga ttttgcacca agcccgctta cactctgagt
     1621 atcccaaacg ttcagtaatt atggagcgtg attgggacaa gtttattgaa tctggccaga
     1681 aaattacgga ccaacagctt gaagaaattg aaaaaagcgt tcaattcgat gagcctgtaa
     1741 atattcaata tacttcaggt acaacagggt ttcccaaagg ggcaacgctt tcgcataaca
     1801 atattttgaa caatggcttt tttattggcg aaaggcttta ttatactgaa aaagaccggg
     1861 tttgtattcc tgtgcctttt tatcattgtt ttgggatggt gcttgcaaat cttgctatta
     1921 ccacgcatgg ggcttgtatg gtggtccctt ccgagtcttt tgagcctgaa atagtgctta
     1981 aaactgttga agcagagaaa tgtacctcgt tatatggtgt ccctacaatg tttatagctg
     2041 agctggagca tccaaacttt agtaaatata attattcaac cctacgtact ggcatcatgg
     2101 caggttcacc ttgccctgtc gaaactatga aacaggtcca gagcaaaatg aatatgaccc
     2161 aggtggctat ttcatacggg atgaccgaaa cctcgccagt atcaaccatg acatttgctg
     2221 atgaccgttt agacaagcgg gtttcaactg ttggcacacc gttgccttat attgaggtca
     2281 aaatcattga ccctaaaaca ggagagattg tacctcgtaa tacaaaaggt gagctttgta
     2341 cacgtgggta ttctgtaatg ttaggatatt gggacaatga agctgacacc cgtcttgcta
     2401 ttgatgctgc cgggtggatg cataccggcg accttgctac catggacgat gatggatact
     2461 ttgctatcgt tggtcgtatc aaagatatta tcattcgcgg gggagaaaac atttcacctc
     2521 gcgaaattga ggaatttctt atccgtcacg aggctatttc ggatgtgcat gtcattgggg
     2581 tcccgagtca gaaatatggt gaagaggtaa tggcctgggt taagaaaaaa gaaggtatgg
     2641 agcttacaga ggaggaattg aactcgtatt gtaaaggcca gatttcaact ttcaaaattc
     2701 caaaatactg gaagtttgta gattcgttcc cgatgaccat cagtggcaaa atccgaaaag
     2761 ttgaaatgcg tgagatatcg gccaaagagc ttgggcttga acagttcaac cagttcaaag
     2821 gcaacgggaa taatgaataa tgaagagtga ataatgtaat atttgctaaa gattaaatta
     2881 gctgcataat tgaggcattc attttcatcg aaaagaaaca ttgaaaaggt ttaaatacaa
     2941 ctgaaaatat ggaagccagt ctgattagag ttcaggctgg cttttgttta aagagctgaa
     3001 tttcaatgtc ttggtttttg gtttttgatt cttgattctt gattctcaat taatgccttg
     3061 tatatttcat tatttatgaa taaaatacct aactttacca cttctttgaa ataaagttaa
     3121 ttatttgata tttaataata tacatcatgg caaaagaaaa aattgtactt gcgtatagtg
     3181 gagggttaga cacctcagtt atactcaaat ggcttctcga caaaaattac gagattattg
     3241 catacattgc agacgttgga caagatgaag atttcgaagc tgcaaaggaa aaagctttaa
     3301 aaattggcgc atcaaaggta tatattgaag atttgaagcg agagcttgtt accgattatg
     3361 tatttcctgc aattaaagcc aatgctattt atgaaggccg ctatatgctt ggtacttcat
     3421 tagcacgtcc aattattgcc aaacgtcaga ttgaaattgc tgcggccgag aatgctgctt
     3481 atgttggtca cggtgcaacg ggtaaaggta acgaccaggt taggtttgag cttacctact
     3541 atgcgcttaa cccatctatc aaggtaattg ctccctggaa gatgcaagag ttccttgatc
     3601 agttcaaagg gcgtaccgat atgctcaaat atgccgaaga aaaaggtatt cctgtaaaag
     3661 ctactttgaa aaagccatat agcgaagatg acaacctaat tcatatcagt cacgaagcag
     3721 gtattttgga agacccatta cacccttgga gtgcagatat gttgaccaaa atggttcgtc
     3781 cacaagatgc tccagataaa gaaactaagt tggccatcta tttcaaagac ggtatccctg
     3841 ttaaggtggt taacaaagat gacggtacaa ctgttacaga tccattagaa ttgcttaatt
     3901 acgttaataa aattggcggt gagaatggta ttggcatttg cgatatggtt gaaaaccgct
     3961 tcattggtat caaatcacgt ggtgtttatg aaactcccgg ttatactatt cttcgtactg
     4021 cccatatgga tatcgaaggt gtagcaatgg accgtgaagt gatgcgctta cgtaatatgt
     4081 tggctgagaa gtttgcagag cttatttaca atggtttttg gtattctccg gagatggact
     4141 ttttattggc tgccattaac aagagccagg aattgattga tggtgtggta tatgttaccc
     4201 tttacaaagg gaatgtattg gttgatggac gtgagtcacc aagctcattg tacaatcagg
     4261 atttatcaag tatggatatt gaaggtggct ttgaccaaaa agacagcgct ggttttatca
     4321 agatcaatgc agtacgtctt atggctcaca atgctattgt ccgcagacaa aagaaatagt
     4381 agcactacaa ataaacaaag agcgtaacct tatggttacg cttttgttca aatcaatatc
     4441 tcaaaaatta tttgctgcaa atagcgcccg gcttttttaa aatcctgtgt actttgcggt
     4501 ttaattttta ttggctaaat gcttaatttg tttgtacaat taacgcattc tttacttccc
     4561 aaattcaaag cctaccatga tatagaaaat gggagggaat gaaatatcaa catatccgtc
     4621 accaccaaca ggctgactta tgaatccaaa ttcgccaatg aaaccttttc cacttggcag
     4681 gctttttgca aactggagcg taaagccaac atttagcccg tcactgttat ctctctatca
     4741 tcattattga tatagttata tcctacaaac aaaccgggtt ttatttgcat atcggtaccg
     4801 gcattgaaaa ttggtttgat ggttccccca atagacatgt aattactgct ttcatcatag
     4861 ccgtcaacac ccaagagccc aaatatggct gaaaccccca tggaaagttt ggggacaagg
     4921 ataccatcca catccatctt tactaaagga gaaatttcgg gtttcatttc gtatcctccg
     4981 acattgattt ttccggggcc ggtaagcaaa agccctgctt caatgcttat tgaatagctt
     5041 tccctgcctt gagacgatat aggtgaagat tttgaagagc tgttagtttt ggcttcttct
     5101 ttcaaaattt tatctacttc atccatgttg taaacaaaaa tacttttgtc ggctgtttgg
     5161 attttgatag attggttggg tacctgttcc atgattaccc cgcgtattac agacccattt
     5221 ttgaggtaaa ccacgtcaat gtattgctgt gctaacatgt ttatggaggt tagcagcaaa
     5281 atagctgaaa tcaaaaatat ccttttcatg ttttttttgg aatttatggt tttacaataa
     5341 ataaacacta agtgatatat gattgctgta gctttgaaat actgaaaaga acgaatagct
     5401 tattcatgcc gattggttaa caagaatttc tgaataattc gttttgctct tattctgaaa
     5461 tagttaatcc ataggccccg acaatcgggg tttcgtaagc tcaatattta ctatacctgc
     5521 taaaagaatt catttcatga acctttatag cagttttgta taaagttacg aaaattaaaa
     5581 gcaggttgta tttattatcg tcaaaaatgt taaagtaata aaaaaattta tgattaagac
     5641 tttttagtct ttccactgag cttcattttt tatttggcat taaaattgcg tgattaaact
     5701 tcttaaataa ttaataatca tggaagcatt tgaatcaaac aacagggaaa tcttatcgtt
     5761 aattgaagtt tgggagccaa agcttttagc tttgagcaat gacgttattt ctaacaagcg
     5821 taatgtacaa aaccggacta taaagcagat tttgggacac ctggttgatt cggcttccaa
     5881 taatactcat cgcattatcc atttacagta tcagttcaat ccgttggtgt tccccgatta
     5941 tgctaacctt ggcaataatg accggtggat ttcaatccaa aactatcagg gagaggattg
     6001 gggaacgatt gtgcagcttt ggaaatatgc aaatcttcat ttagtgcata ttatacgcaa
     6061 tgtaaattcc gaaaaacttg aaaatgaatg gatttctgcc ttaggcgaaa aagtaaagct
     6121 aaaagatatg ataatagatt atcttaggca tttcaagttg catttgggcg agatagagga
     6181 gctgataaat aagtaactaa aatatgttta ctactcctgt ttcctgcctg agaagagaca
     6241 aactctgctt ataaagagag gagtaaaagt acattgttca aatattttgg tttatttaat
     6301 ttcatccttt ctctaatgta aaagagaatg tagtgcccac gccaacagca ctacgtacgt
     6361 ggatattttg attatgagat tcgatgatgt gctttacaat agccaatcca aggcctgaac
     6421 ctccaaggtg acgactacgg cttttatcta cccgatagaa acgttcaaac aatcttgata
     6481 aatgtttttc ttcaatccca ataccattat cagaaatttc aacgagtaca aggttatcca
     6541 tgtaatacac ggtaaccttg gttcgcccgt tattattgcc atagttgatg gaattttcga
     6601 caatgttgtg tattacctgg cggatacgct ccttgtcggc tacaacagtt atgctgccta
     6661 attcgcgggt tttttcagct atagtgagtt caatattttt gttcttagct ttaatttcga
     6721 gcatttcgaa cacttcattg acaagggaca acaaatcgaa tttactgtaa atcattttta
     6781 tttcgcctga ttcgagttgc gaaataattt ccaggtcttt gacaatatta atcatccgtt
     6841 cgatgttctt gtctgttttt tcaaggtatt catgattaac ttccgggtca tctaaagctc
     6901 catccagtaa agtaagtaca tatccttgta tgttaaacaa tggtgttttg agctcgtgtg
     6961 aaacatttgc caaaaactcg cgacggtaaa cttctaattt tcgcagttct tctatttctt
     7021 ccttacgttg ggttgcccat ttttcaacat cttcttgtac ctttgcaata atatcttctt
     7081 tcaggttgac tgtttttatt ttttggtctt tgttaagttt cagggtgtgg atagttttgt
     7141 atatcagctt gattttgtcg taaatatacc tttctaacac gtaactgaat actaataccg
     7201 ttaacccaaa gatgataatg gaagatgata tgaggacaat aaaactgaat tgtttaaaaa
     7261 acaacaagtt taaagctata tatataagag catagcctcc aaccacaata aatgccccac
     7321 gtagcagcac cctgtatggg ttggtcgatt tcatgatggg tttattcatc ttctgtttca
     7381 tatttgtaac ccacgccttt gatggttttt atacagtcat tattaagttt ttctcgtatt
     7441 ttgcggatgt gtacgtctat agtcctgtcg ccaacaataa catcgcctgt ccagatgcgg
     7501 ttatatattt cgtcacgggt aaagaccttg ttcggcttgg aggctaaaag gtgaagcagc
     7561 tcaaattctt ttcttggcag ggtaatttca ttttcattaa taataaccac gtacctttca
     7621 gtgtcaatag tgacgttgcc caatgtaatc ataggccgtg caggtttgct attgtctgaa
     7681 tatcttctga gcagggcatt tactttactc accaacacgc gaggcttgat aggtttggca
     7741 atataatcat cagctccggc atcaagacct gctactaaag aataatcttc attacgggcg
     7801 gtaaaaaatg tgataatagt gctggataat gaagatattt gccgtatttc ccgacaagtc
     7861 tctataccat ccatccctgg catcatcacg tcaaggatga taaggtgtgg aatttgttcg
     7921 cgggcaatag ctactgcctc tgctccatta taagctttga ggactttgta accctctttt
     7981 ttcaggttat agcttacgaa ttcgataata tcttcttcgt cgtcaacaat tagtattttg
     8041 tattgttcgg tacggctcat aatggttgca atttaactat tgtttgtcaa tttttctttt
     8101 tcttttttct ttttgtgccg caaaacaaca gcttcgcggt aaaacacgat ctcttcggca
     8161 atatcttttg acaaatcgcc tatccgttca agttttttga tggccaatag caggtttaac
     8221 aagttaatgg tttgttcggg gtattctttt attagttcgg caatgacatc gaccgatttt
     8281 ttgttgattt tgtccagctc gttgtcttcg tgaaagatag cacggaccga atggtttttt
     8341 tcatcttcaa aagcttcgcc agttgccata accatgttag tggctaatgt aaacatacgg
     8401 tcaaactgaa ttctctcagt acacaccttg ctaaactctt gttctgcttt gataacaagt
     8461 tttgcaatac cccgggcata atcaccaatg cgttcaaggc tatagttgat gcggagtact
     8521 gaaagtacaa accgcaaatc attggctacc gggttaaaca aggccatgat attttcgcag
     8581 tccatgcaaa tcttgagctc aaaagcatca agcctttttt cgttagcaat aacctcatga
     8641 gccagctctt tgtcgttgtt ttcaaaggct ttttgtgccc tgtgaagctg acttatggta
     8701 aggccccaca tgtcattgag ggaagctctt aactgctgaa gttcggtgtc taagtgggtc
     8761 atggtatttt aatttgaaaa tgtgataatt agctaatttg aaaatttttc tgcagatttg
     8821 ctgcggcgag gctgagtttt tcactaagtg cgccgcagcg cagcagagaa ttagtagcca
     8881 ttcatttttt cttccttgtt cctctgagtt ctctgtgcct gagtgttagt ctcttttatc
     8941 caaaccttcc cgtgatataa ttttgtgttc tttcgttggc cggattggta aatatctttt
     9001 tagtttcgtc ccattctatc agttcgccaa ggtaaaagaa tgctgtatag tcgcttacac
     9061 ggcctgcttg ctgcatgttg tgtgttacaa tgacaatggt gtagtcgttt ttcagttcgt
     9121 atattagttc ttctattttg cctgttgaga tggggtcgag ggcagatgca ggttcgtcca
     9181 tcaggatgat agaaggcttt atggccagtg tgcgggctat gcaaagcctt tgttgctggc
     9241 cgccagacaa tgccatagcc gattgcttaa gcttatcttt aacttcatcc cacaaagcgg
     9301 cctgtttgag cgagttgaca acagtatgct caattttatc tttgtcgttt atgccattga
     9361 cgcgcaagcc atacgctaca ttttcaaaaa tggattttgg aaaaggattt ggcttttgga
     9421 acaccatgcc taccttcttg cgcaggtcgt caacgattat cccttttttg taaatatctt
     9481 ccccttcgac caacaccttg cctgtcatgc gtacatcatc aatgaggtcg ttcattcggt
     9541 tgaatgtacg taatagcgtg gatttgccac atcctgaggg gcctataaat gctgtaactg
     9601 tgtttttctt tgcatgaaaa ttgatatttt tcagtgcatg aaaatcatca taaaacaggt
     9661 ttaaatcttg tacttctatt tccttcttat ctttagacat atatcttaat tttaccaccc
     9721 ttacctccag cctgaggcgg gacaagctct ccttgtgaag gcggggaaat gattcttcgt
     9781 ttaaaactat cttataattc ttaaatttat tagtaattag tcatttgtaa ttactttctg
     9841 atatttattt ttctattgaa ataccgtcgt atcagggctg ctatcaagtt aaaaatgagt
     9901 acgatggata tgagcactaa ggctgtacca taagccattt tttgcgaaag ttcaatgttt
     9961 gtaccacttg tggtgataac atacaggtga taaggcagtg ccattacctg gtcaaaaatg
    10021 ctggtgggca atttgggcag aaaatatgcg gctactgtaa ataagatagg tgcagtttca
    10081 ccagatacac ggcttataga aagtattaat cctgtaatta tattagggaa agcaattggt
    10141 aacactactt ttcggatagt ttgcaattta gaagctccaa gggcatagct tcctgtacgt
    10201 aacgaatcat caacactttt cagggcttct tcggtagttc ttattattac cggaagaacc
    10261 attagcccaa gtgtaagaga acctgcaata atagagtctc caaactttaa ggtgtttaca
    10321 aacaaagcca tcccgaacag gccaaagacg atagaaggga ttccggcaag gttgtcggtc
    10381 atattgcgga aaaaacgctt cattggcgag tctttcatgt attcgtgaat gtaaatgcct
    10441 gacattacgc caataggaaa cgcaaaaata gcgctaccta ttatcaaaca gaatgttccg
    10501 acaatggcgg gcaggatacc cccttcggtc atgccatttt cgggcatagc agtcaggaaa
    10561 gtccagttga tggcttttgc acctctaaag atgataaaac ccaggatgac aaagaggatg
    10621 gctactacca taaagctgac aatacgcaaa atggtaaaag ctatattttg ttcgatttgt
    10681 ttctttttta tcatgtttta cctcctgttt cgggctttaa tactgttgac gataaggtta
    10741 aacccaaagg tgataatgaa taatactatc cctaaggcaa acaatgcttt gaaatgtgtt
    10801 ccgccaaaag gggcttcgcc aagttcggca gctatagtag ccggaatggt gcgcactggt
    10861 tgaaggaaag tatgcggtat aacagatgca ttgccggtta ccatcagtac tgccatggtt
    10921 tcgcccatag cacggccgat gccaagaatg gcagctgctg taatacccga ttttgaatat
    10981 ggtatagtca ccctgtataa agtttgccaa cgactggctc ctaaagccag gcttgcttct
    11041 ttaagtgccc ttggggtaga tgacagtgca tcttctgata tggtgatgat ggtgggcaat
    11101 gccatgatgg ccaggataat gctccctgcc agtgctgttt cgccaacgct tagcccaaaa
    11161 acatcatgaa tgataggtac tataacaacc agtccaaaga aaccatagac tactgaaggg
    11221 attccggcaa gtagttcaat gacaggtttt agaatgtctc gtacacgttt gtcggccact
    11281 tcggtaaggt atagtgcagt aacaagcccg attggcaaag ccagtaaaat agcgcccaaa
    11341 ctaacccata atgtgccaag tatcagtggc aatgcgccaa actgggctgc tggggttgct
    11401 gttggaaacc attctttgcc ccctaaaaag tcaaataagc ctattttttt tatttcgata
    11461 tgtttgcctt tgaaattttt tggcaaactg ctgctgtgca tgtatgcaat gatgccagta
    11521 tgagagtcaa ttaattcgtt gattttgtcg ggcataaact ctaagtcggc accaagttct
    11581 tcttcagtgt agtagtttgt aatgtcgttc agcgaaaata ttataatgct gtcgtctttg
    11641 ccacctactt gtttccagtt ggtaatttcc tggtcaaaaa tatcttttat ctgcgcttca
    11701 gaaaggatat ttacagggtt ttctttattg acagacaata cgtacccatg ttctacaggc
    11761 ttgtcgttga aaaggctgat tccttcttta aataagaaaa tgataataag cagcaccgtc
    11821 aacgtagtaa cggtactgct tgcaaataaa atggattctg ttattttctc aaacttacgt
    11881 ctaagcttca tttgattttc tttattctat gttctttgct cttttttctc tacttaacag
    11941 gcacataccc ttcgtcaagt actattttct ggccttcggc agatagtaca aagtcaacat
    12001 aaggttttat cttactttca acatctgtag tgtagtaata gaacaaagga cgggtaatag
    12061 gatatgattt gtctttggcc gttgcaacag atggggcaac ataagtttta cctttatcat
    12121 aagacacttt caaagctttt acgttttttt cgacataagc aaggcctaca taacctattg
    12181 ctccggctgt ttggcttact gattgcacaa tagcgccggt ggcaggcatt aacaatgccg
    12241 atggggcaaa gtttttcttg ttcatcacgt gttctttgaa aaactcataa gtaccagagc
    12301 tggtttcgcg tgagtaaaca ataattttga ggtcatcgcc gcccacttct ttccagttgg
    12361 tgatttttcc ggtgaaaata ccttcgagct gttcgcgggt aagttggctc actttgttgc
    12421 cagggtttac gataacggca agggcatcaa gggcaatagt tacttctttg tatgcctttc
    12481 cggcatcttt gagtttaatt ttttcgtcca tcttcatcga acgcgaagat tgtgcaatgt
    12541 ctgttgtacc gcttaataac gcagacaatc caactccaga gccgcctcct gttacagaaa
    12601 ctgatgagcc tgtttttttg ttaaaagttt cagcttcttt ttgcgaaagc ggcaagacgg
    12661 tgtcgctgcc tttgattttg cacgctactt tttgggcaat ggcagttgta agtgttaagc
    12721 ctgcaagcac tgctactgat aaaattattt tcttcatttt aatttcattt taaatttatg
    12781 taaaagaaca accggaataa aaccttaatg ttattctaat attaagttat tgttaagcgt
    12841 atttctgtct ttatcaaaat ttgtacaata cccttaaagt taatacatta tctttcaaat
    12901 cggaagtata atttttaacc ccgttttcgg caataacggc cttgttatat ttggcattga
    12961 gtttttcgtt gcttaccata tcatagtaaa cgctcaggcg gacgttaggg gtggcgtagt
    13021 aattgtagcc cagcccgatg gtggtgaatt tcaggtctga agggccaaca tttttgtttt
    13081 tgcttacaag ctcgtcgcct gaagcatcgg tgttggggtc gtaccagtcg tatttggcca
    13141 caatctcgtg cttggtttgc atcagcctgt gtacaagcat tacgtaaccg ccgttgaagt
    13201 tacgcaggta aatgtcaggc ttgactgtag cggtataagt tactgcattg cccagggtgt
    13261 cttttatgat gtagtctttg gtgtcggtaa tagcgccttt ggggctgact gtagatttga
    13321 aagtgcctgg ctgttgtccc cacaggtact ctgcacgtaa agaagttaat ccaagagctg
    13381 agttcaatga tatttgggca tcaattccaa aatatttgcg gtcgatgcgg tcgcctttct
    13441 tatagcctgc atcggctttg aaagttgagt cggcgctgtt ccatttcatc aaagtgccag
    13501 aaggactggc aaatccgcct aagtaatatg aaatgccccc gctgatttgc aggttttcgt
    13561 ccatcaatac ttttttgaaa gaaagatggg ctataaggtc tttgaatttg tcggtttcag
    13621 aggtaatggc attgccattg aaaagtccta catcgagttt caaaaaattc catgacgagg
    13681 tttttggggc ctggatggtg agtttagcgc ccaggtcgca ttcgtcgggg aaaagcgttt
    13741 gcaaaaccct tgcacgttcg gggctttcta aggttgaaga agagtattct acctcaaagc
    13801 caatgggcct gttgaaaata cccccggtca acgaaaacgc atttaaaagt ggttcggtgt
    13861 atatcccgta agcttctttg acttttaccg agccgtcatc tttcaggtcg agctgcatgg
    13921 ctatattaga attagtcccc gagtaagcag ctttcaaacg agcccttctt acctgaaaac
    13981 ggttgtcggt gcctttaaag tcgccaccgg ctactgacgg gctgccaatg gtatcggctt
    14041 tttgccattg ggcctggatg tatcctgaga atttcagttt tttgagcagt ccaatttcag
    14101 tttcggcggt ttctattttt ccagatagcc attgtactga gtcagtgatt tcttcttctt
    14161 gggcttggac aatatttact gttaaaaagc ccagcatggc gaaaagtact tttttcatat
    14221 taatttcatt ttaaatttat aatattctaa ttattaatat tttgcgaatt gtttcacaaa
    14281 aattcatggg acaaaattaa ggctggatta acaaaaacca atattttgaa aaatcttcta
    14341 aaactcaaca atgataatgc ttatagtcga aatttaacac acgttttacg aagacttaac
    14401 gtaatgataa cattcggcca tagtataatt taccctttct taaagtgggc ttaacaatta
    14461 cattacatac gcagcctaat tttgctttca aatcaaaaat gaaaatgtta tgaaaagatt
    14521 tgcaagtttt attattagcg gaattttgtg tgttagtatg ttggcatccg aaaagccaag
    14581 tgccaacgtt aaagcagaat caaaagttga aaatgctctt atttcgggag ttgtggtgga
    14641 caaaatcact ggcgagcctt tggctggcgt tgaggtaaaa atgattggca gcaacgaaaa
    14701 ggtgtttacc gattttgatg gcaattttca gtttaccaat attccacaag gtgcctatgc
    14761 tataaaaata gattatatct cttatgaaaa tgtagtagaa aatgtttacg ctacaaaaga
    14821 agaaaaccct ataaaagtaa aactttcaag cgtaaaataa ccaacgctca attctattaa
    14881 atattttcca ttgttcaaag caggcaacag ctaagctgac ctgcttttaa attgaaaaaa
    14941 ctatgacgaa gaaaacattg ttcttcctga tggtattggc tatgccatgc ctttcggtag
    15001 ctggacagga tatcatccgc gttgagcaaa ccaaatattt tgacaagaac cacaacctct
    15061 acaacggggc ttacaccgaa tactatgaaa acgggcacaa acgcctcgaa atgaaactga
    15121 aaaacggcga acaggattct gttacggtgc tgtacttcga gaacgggcag ataaacgaaa
    15181 cccgctcata caaagatggc ctgatggatg gccgttggga agtgtttgac gaaagtggca
    15241 acaagctttc tgaagctttt tacaaaaaca acaaaaaaga tggctgctgg cgcatctgga
    15301 atgccaaagg ggttttgctt tttgaaatgc attataaaga tggcgtgaag tgcggcgatt
    15361 ggaagacctt caacgaagat ggcactgtaa agtcacaacg gagttatgaa gcgtgtttgt
    15421 aaactttaat tgaccaacca tggaattaat cagtgtagaa gatgttgcaa aagcagccaa
    15481 cctcaaaggc cccggctcaa aaggcgtggc aaaggtgttg atgtcggcct ttaagctcaa
    15541 caaattaaac gagctttatg aaaaacatgc cgataccaaa ggctttgatt ttgtaaatat
    15601 gatattgcag gaaaccggga ttaaatacaa ggtgccggtt accgatttgg aaagggtccc
    15661 taaaaccggg ccgtttatta tcatttcaaa ccatccgtat ggcggggccg aagggcttat
    15721 tttgcttgat gctttgaaaa acatcaggcc tgatataaaa atcattgcca acttcttgtt
    15781 tcaaaaaata gaacctctca acgagtttat ttttcccgtc aatccgtttg aaacatatcg
    15841 acaagtatcg agttttcaag ggttaaaaga agctatgttg catttgagca atgagcaccc
    15901 gatacttata tttcctgccg gcgaagtatc tacgttttac ccgggaagta cggctgtggc
    15961 agatagaaaa tggagcaaac cggccttgaa gtttataaaa aaagtcaatg tgcctgtggt
    16021 ccctgtattt ttcaaaggaa ccaacagtca tttgttccat ctgctgggca tgatacaccc
    16081 taccttgcgc acagggcgat tgccagccga gttgcttaat aaaaaggaca aacctgtaaa
    16141 cctccggatt ggctcggcca ttacggtgaa ggaacaggca aagttcagca gcgtatctat
    16201 ttacggcaga tatctaaggg ccagggttta cgccctcgat acatctgagg atgtaaaaaa
    16261 attttttttt ccaaaaaggt cagaaagctc aaaacatgaa gtagtcagcg ctgttgaccc
    16321 tttggtttta caacaagaga taaatgcctt gcctgctcat tataaggtta ccggacaaaa
    16381 taagctggag gtgtattgtg ccccgtcttc ggctattccc aacatgatta ccgaaatagg
    16441 ccgtatgcgc gagattacct ttcgcgaagt gggcgaaggc acgaatatgt ctatcgacct
    16501 tgacgagtac gacttgtatt accaccacct gtttatctgg catgccgatg acaaaaagct
    16561 ggtaggggct tatcgtcttg gctttgggca agatatcatt gataaatatg gcattaaggg
    16621 tttttatacc cgcagccttt ttaagtttaa aaaagaaatt tacccaattt ttacgcacgc
    16681 cattgaagtg ggacgttctt ttgtcatcaa agaataccaa aaaagccctt tgcctttatt
    16741 tctgctctgg aaaggcatat tcctgacgtt ggtcaaaagg cctgattatc agtacctcat
    16801 tgggccggta agcataagca atgactattc aaaggtgtct aaacggattc ttattgacta
    16861 cattacccgc aaccatttca ataacgaatt ggccaaatat gtcaagccac gcaaaaagtt
    16921 taaagtcagg ttccccaatg tggacaccga aatattgtct gatacagcta cagaccttgt
    16981 caatattgat aaagtgattg ccgacatcga acacgacagg tttaaaatgc ccgtgttgct
    17041 caaaaaatac atccagctca atggcaaaat tgtagctttc aacatcgacc caaagtttaa
    17101 cgatgccctc gacggcctgc ttatattgaa ttttcatgat actccgcgcg aaatgattga
    17161 ctatctctcg cgcgaaagtg aaactaaatc ttaatcataa atacctcaga caatgtatag
    17221 aaatatgtac tataaaattt taacagttat tttctcttta tttttgtgct cttatgcgca
    17281 aaataccgcc ggacaggcaa gcatttcggg tacagttacc gaccagaaaa caggcgaaac
    17341 cctcattggc tgcaatgttt acatacaggg cactaccaat ggcgttagca ctgaccttga
    17401 cggtaattat tctttaaaag ggctttctgc aggcacttac acgcttgtgg cctcttatat
    17461 ttcttataat actgcaacta tatcagtaac agtgcctaaa aatggcgata ttaaagctga
    17521 ttttaaactc gaaccggtta cagttgcaat caacgaggtt actgtggttg cccgaaaacg
    17581 tcaaaataca gaaatgtcga tgatttcggc cataaaaaca caagacctca ttgtcaatgg
    17641 catttcgggc caacagatac aaaagtcgca agaccgcgat gctgctgagg tgctaaggcg
    17701 catccctggc attaccatta ccgatggcag gtttgtgata gtacgcgggc ttatagaacg
    17761 ctacaactcg gtactgctca atggtgtgac tgcaccaagc tctgaagccg atgcacgtgc
    17821 tttctcgttc gatgtgatgc ccagcaacct tattgataat atcttgattt acaaaacccc
    17881 tgctcctgaa ctccctgctg attatgccgg tgccgctatc aatgtgatta ccaaaaatac
    17941 ggtggaagaa aacagcactt ctttttcgta tggcattggc tatgttacca atactacgtt
    18001 taaagatttt tatacctata aaggaggaaa gtacgactgg cttggctttg acgatggcac
    18061 aagggcactt ccttctgcat tcccggcacg taatgtaatg aacgattcgc tcctcaattt
    18121 cgactataaa cctggcatgg cagactctat tgccaaaata tcaaaggctt tccgcaatga
    18181 cgtttttgcc ccctttacca aagtggccaa acctaaccag agcctggccc tgaacatcaa
    18241 caggaggttc aaaatcggga aaatggatgt ttctaacctt acttctctca tgtataacta
    18301 tagcgcttct actcaggatg caaccagggc cgaataccag gatcctaacc ctattacagg
    18361 aagcaaggaa tacagctact tgtttaatga catgcgttac aatgaggctg tgaaaacggg
    18421 catcatgcac aactggactt ttgctttcaa cgagaaccat aaaatcgaat ttcgcaactt
    18481 cctcaatcaa aacagccgga accgtactgt aatgcgcgaa ggggtaaatt attacacggg
    18541 ttctttgcca gataccatca aggccattgc tatggaattt acgcaacgta caacttattc
    18601 gggacagttg gccggagtgt ctgagtttaa cgacaaaaat acccagttgg aatggatact
    18661 tgggtattct tatgccgaca agcttcaacc cgattttaaa cgcttattct ttgtaaaaca
    18721 agaagatagc gaaggtcagt atcgcttccg catgagtatt gctaatggct cccctcttcc
    18781 tgagcggggt ggaaggttct atcttgaggc ttatgagcat attaaaaatt tggcagttaa
    18841 ttttaaacaa aaccttaata ttttacaggg aaagacaacc ctgcgcacag ggatatttta
    18901 cgaacagcgc aaccgcgagt ttgacgcccg caatattggc attgtattta aaggggctta
    18961 cccgacagat atgtttgcgc ctatcgacag catcttgcaa gacaaaaact tccgctaccc
    19021 cggtggtttt gcttataacg aaaattcgcc ccggacaaac cactataaag cggtaagtag
    19081 tactgctgcc gcatatttgg gagtaaaggt gcctgttttg aaacgctttg tgtggtactc
    19141 aggcctcagg gctgaaaatt atgagcgcga agtgtccgaa tttcagttgg acgttaaaaa
    19201 tgtgcctaat atcaaattag atacactcga cttgtttttc tcctctaacc tgagctataa
    19261 tatcaacgaa aaacatatta tcaggctttc gtatggaaaa accgtcaacc gtccagagtt
    19321 tcgcgaaatt gtgccttttt attacaaaga ctttgacctc aatgccggcg tttggggcaa
    19381 cgataccatc gaaaactgtt atatccacaa ctacgacctg cgttacgaat ggtatccttc
    19441 gccttccgag atgatttcgc tggctttatt ttacaaagac ttcgtcaacc ctattgaggt
    19501 atcgttgatt gaaaccggta accaacccga ttacaagcct tttaacacag caagtgctta
    19561 tagccgcggt attgagctgg atgtacgaaa aagctttgaa tttttgtcgg acaatacagg
    19621 ctttttgtac tatttcaaaa actttaccct tgtttttaat gcttcgttga tagaaagtgt
    19681 ggtaaagacg aatgtgcctg atgcccgcga aaaagaacgc gaaatgatgg gacagtcgcc
    19741 gtatattatc aacggcggtt tgtatttcaa cagcgacaaa caaaagctga tgctgagctt
    19801 ggtgtataac cgcattggcc gtaggcttgc tgctatcggg actaaaaaca cccctcacgt
    19861 gtgggaactt gcccgcaacg gcctcgactt tacccttacc aagggcttgt acaaaggcat
    19921 agaactgcgc cttggtatta aagatatact caatgaacct atccgccgcc tgcaatacga
    19981 acaaacaaac ctgagcccta ccggccagca tgtcgagatt gagcaaacca ctctcgaata
    20041 caagcccggg acaacgtatt ctgttgcttt atcatataag ttttaagaca gtcatttttg
    20101 attattcttt caagccatcc tgttcgctcg gggtggcttt tttgtgaagt gcaccaaact
    20161 ttcataaccc tgcaaaacct ttagcaacat ttgtagaggg tccattcgcc ctgcttttga
    20221 tacaaacaaa atactaaata tgcagtattt gtaaaggctc aagtttatta ttttctgaaa
    20281 tgttttgcca gcgtggtggc aaaaatgaat tcattgagta actcattgcc agagttgaaa
    20341 atgtcatcct ttgtaccttc ccaggccttg gtcccctggt gaagaaatac cactttctct
    20401 cccagttcta atactgagtt catatcgtgg gtattgagga tggtagtgat attaaattct
    20461 ttggtgatat cttttatcaa ctggtcaata accgtggccg tctgtgggtc aagcccggaa
    20521 ttgggctcat cgcaaaacaa ataacgaggg tttagcacaa tagcccgtgc tatggctgcc
    20581 cgcttttgca ttcccccgct tagctgcgag ggatagagct tcccggcatt ctcgaggttt
    20641 accctctcta agcaatacct cacgcgctct tttttttctt tttcagtcat ctgagtaaac
    20701 atgtcgagag gaaacttaat attttcaaca acagtatatg aatcaaacaa cgccccgccc
    20761 tgaaataaca tgccaatatt tttgcgcaat tcgcgaagct cggccgatga ctgggtatta
    20821 agcgaaatac catcaaataa gacctctccc tcatcaggct gatatattcc aaccattgtt
    20881 ttcaataata cggttttccc cgaaccgctt tgtccaataa tcaggttcgt tttcccttct
    20941 tcgtaattga cccaaatatt acgaagtact tcattgccgt taaacctttt tgaaatattg
    21001 cggatttcta tcatgtcaac aaaaattggg ttaacaacaa attgaaaaaa aggatggcaa
    21061 tactactata tacaaccgct ttggtacttg ccttgccaat ctcaacagaa ccgcctgtta
    21121 cataatagcc attgtatgca gatattgaag taataagaaa tgcaaatacc actgttttga
    21181 taagcccata tactacataa taaggcttaa acgaatactg caacccgtaa atgtattctt
    21241 cagaagtaac cacacccgta gcaaaaccag ccacccagcc tcctacaatc cctaccaaaa
    21301 tacttatcac tgtaagtaca gggttgaaaa taacagcagc aaaaattttt ggcatagcta
    21361 taaaaccagc cgagttaata cccataattt ctaaagcgtc tatctgctct gacattcgca
    21421 tgaccccaat ttcggatgca atatttgaac caactttgcc tgccaaaatt aaagctacaa
    21481 tggtagatga aaattctaat atgatagaat cccgaacggc caaagctatc aggtaattgg
    21541 gcagaagcgg cgtgtctgcg ttaataccag tctggattgt caacacagca ccaataaaaa
    21601 ccgaaatgat ggctacaatg ccaatagagc taaacccgat attctcaatc tctttaataa
    21661 gttgtctgcc ataaattctt attttttctg gctttacaaa gactttgccc agaaaaatga
    21721 agtagcgccc tatgtggtaa aaaaatccca tgaaatgagc atgtttagaa gtaaacacaa
    21781 acacgtatga tcccccattt gtcgggacaa gttccatccg acctttaaaa ttcaattttg
    21841 ttcggatgaa aggtttgttt ttcactctaa agatacaaat attggagaat gtatgaagaa
    21901 agattttttt aaaagagaaa aaaataaaaa ctttacagcc tttaaaagtt ggattcaaat
    21961 tattttattt tgcaatggga gtaaaaaata acaattattg tattatcatg gccggtggta
    22021 ttggtagccg tttttggcca tacagccgta ccgcctgtcc aaaacagttt ctggatatac
    22081 tgggcttagg gtcaactttg ttacaacaaa catacaaccg ttttgcccgt ataatgccac
    22141 acgaaaacat tcttattgtt accaatgccg aatacaagga gattgttcaa aaacaaatcc
    22201 ctcaagtgcc ggttgacaat atattacttg aaccagcacg ccgaaatact gccccttgca
    22261 tagcttatgc gtattcaaaa attgcaagcc gtaccaatgt tgctaacatt gttgttgccc
    22321 cctctgacca ccttataata aaggaagatg aatttttaaa tattgttcag gaaagtctcg
    22381 agcttgtttc aaaaaacgac attttaatga cccttggaat taaaccttcc aggccagaaa
    22441 ctggctatgg ctatatccaa attgaaaaag acaacaataa aaaaattggc aatgccaaca
    22501 aagtaaaaac ttttaccgag aaacccaatt acgaccttgc ctgtatgttc ctcgaaagtg
    22561 gagattttta ctggaactct ggtattttct tttggtcgtt aaaaagcatt tccagcgcct
    22621 ttgaaaaaca ccttcccgaa ttatttaact tgtttaataa tgggcatgaa ttctatggta
    22681 ctaaccatga gaaagacttt atagctcaaa tttatccgca atgcgaatct atttccatcg
    22741 actatggcgt aatggaaaaa tcaaacaacg tgtatgttat gctttctgat tttggctggt
    22801 ccgacctggg tacctggggg tcgatgctcg aaaactcaga aaaagacagc aatggcaatt
    22861 atatcagtgg cgaaaatatt tttacatatc aggtaaaaaa atccattgtc aaaaacgaaa
    22921 cccgcaaatg tgtagttgtt aatggtttag aagattatat tgtggtcaat accgaggatg
    22981 tgctgctcat ttgcaggctt aaagatgagc aaatgattaa gcaatttgta gctgatgtaa
    23041 aaatgaaata cggcgagaaa tatttttgat caactgataa acccaaatac ctgtaaaagt
    23101 tttgaaagta caaccttttc tacttcctca aaatttaaag gcttattcag ttctttttcc
    23161 attgaagtta cagatttatc cgtaaatcca catggattga tatgcccaaa atagcttaaa
    23221 tcggtattga cgttaagggc aaagccatgc atggttaccc agtggctgct ttttacccca
    23281 atagcacata ttttcctcgt tttccctggg gtttcagtgt caatccatac tccggtagcg
    23341 ccttgcaagc gttcagcctt aatgccatag gctgcaatag caagtataat ggcttcttcc
    23401 accttataaa tgtattcttt caagcccaat ccgagattcg taaggctaag aataggatac
    23461 ccaaccaatt gccctggtcc atgataagtg atatcaccac ccctgtcaat cttgtaaaaa
    23521 gtggcatttc tggcttgaag ctggatgtag ttgataagca ggttttgctc tttgccactt
    23581 ttacccaacg tgtaaacatg cggatgctgg caaaaaataa ggtgatggag catcttctcg
    23641 ctgcctttgt tcttcaattc aagcacctgg tcaaacaact tttgctgata ttgccatgct
    23701 tccatataat ctgcagtgcc aatatcatga aacgctattt cttcctgatt tttatctgtt
    23761 ttttgaaagc ttattatagt tttttcttcc attttcaaat tatttatctc ttcaatcttc
    23821 caattccccc attttcaaat tccttatttt tcttccattt gctaatttcc caattcgcta
    23881 attgattttt ctcttccatt ctcaaattct caaattttca aattttcaaa ttgacaaatt
    23941 ctcaaattag ctaattacat gatgtgtttc tcagcatgat acgacgaacg taccaatggg
    24001 gcgctttcta caactttaaa accttttgac aaccctattt tcttgtaata cgcaaactgt
    24061 tccgggtgaa tatattcttt tacctcaata ttttcagaag caggttgcaa atactgcccg
    24121 atagtcatga tttgacaatt gacagcccgc aaatcgtcca tcacttcaaa tacttcttcc
    24181 tgggtttctc caagtccaag cataatcccg cttttagcaa ctacccctga ctgtgctata
    24241 tgccctatca cgttcaggct tgtgtcgtac tgtgcttttg tccgtatctg cggggtaagc
    24301 ctgcgtgtag tttcaagatt gtgcgaaatg atgtctggtg ccgcctcaat gaccttgtca
    24361 agatattgta aattgccctg aaaatcgggc acaagtacct ctaatgtagt ttgagggttt
    24421 tcttgtttga ttttttttat tgtataagcc caataagcag ccccgaagtc cgaaaggtcg
    24481 tctctgtcaa ccgaagtaac tacggcatga ttgagcttca tcagctttat agactcagca
    24541 atacgggcag cttcgtccca atctacaggt aaaggcttcc cggtattgac attacaaaac
    24601 ttacaagccc gggtacatat gttaccaaga atcataaaag tagctgttcc cctgctccag
    24661 caatcaccca tgtttgggca gctcccgctc tggcaaatgg tgtgcaactt atgcttgtta
    24721 actatatctt ttactttagc atagtctgct gtagaagcaa gttttatttt cagccaatca
    24781 ggctttcgtt gaatattcat atttttagaa tcaagagtca agaaacaaga gccaagaaca
    24841 agtatataac aatgatatca aggcgtttat atttgagcat ttactactta aaaccagggt
    24901 agtatttccc ctcctttata aggagagctt gtcccgtctc aggcgggagg caggggtggt
    24961 ttaaagtatt tgttataaac gacaatatca aattcatatc tatgcaaaag taaaattcct
    25021 tttaaatatg ctgaaaaact ttctcatatt tgcaatgcaa aaaatagcac atggacaaaa
    25081 acacttttga caaatactgg gccaaagcga tatcctacat tttcaatcct ttaatcatcc
    25141 ctttctttgg cataatcgtc attttatatt ccaacaccta catttcgctc ctcccgtcaa
    25201 acgcaaaata cctcattctt tccattgtat ttacaggaac atttattttg ccactttgtt
    25261 ttgtgcccat gtacatgtat ttgaaagtta tcaaaagtat agacatggaa gacaggctgc
    25321 aacgaatatt gccgctggca gttaccatta ttttgtattc gctgacctat tattttttaa
    25381 tcaaggtatc cattccattt attggttcat tcctgctttc tgtgttgttt accctcatca
    25441 ttttattggt tttactgttt aaatggaaga ttagttttca tactgccgct attggcgggc
    25501 ttacagcact tattttggga ataatgctca ggctcaatgc agatattatg acatggttta
    25561 tttgtagcat tattatttcg gggcttgttg ccactgcacg ccttattctt cagaagcatt
    25621 cgctttttga agtggcaaca ggttacttaa tcggtttttt cacggtattt atctttatca
    25681 taatataaaa gttaatgaag aaaaacatct taaccattag tactcttgta gtaattattt
    25741 ttactattgt tctgctatat ccatcttgca aagtacagca gcctgtccaa acacaggata
    25801 ctgtaaaaat agaaccagct gttgctgtgc ctgatacaga atattatgcc atggtcgaaa
    25861 ccccagccaa atttaacgga ggtgatataa aaagttttat tgcttacctg acaaaaaata
    25921 ttaaatatcc tgctgtggca ttgaaaaaaa ggcaacaagg cacggttgct atacagttcg
    25981 gggtcgattg ttatgggaag gtacacgttt tttcagtgct gaaaagctcg ggagtggcag
    26041 tccttgacaa agaagcttct atggcaataa atgcatctcc ggcatggact cctgccttgg
    26101 acaaaggccg cccggtcggg caattgtttc tgttacaaat caaattcaac gccaagacgc
    26161 gtaaagtaga gataaaatag cattcatttt tttgatagca tttctatcat tatctttgca
    26221 aaaaatatta ataatcattt aaataattga tatgtataaa gtagtattag tacgccatgg
    26281 cgagagtgcc tggaataaag aaaacaggtt caccggatgg accgatgttg atttatctgc
    26341 aaaaggagtt gaagaagcta agaatgccgg gcgtgtgttg aaagccgaag gttatacttt
    26401 tgatttggcc tttacttcaa cacttaaaag agctatccgt acactctggc ttgctacaga
    26461 cgaaatggat ttgttgtggg tcccggtaat taaaagctat acgctaaacg aacgtcacta
    26521 tggtgcgtta caaggacttg ataaatctga gactgctgca aaacacggtg aagagcaagt
    26581 taagatatgg cgtcgtagct atgatgtgcc accaccagca ttggaagaaa cagacccacg
    26641 tcatcctaag tttgatgcac gttacaaaga tatcgaccca aaattactcc ctgcaaccga
    26701 atcattgaaa gaaactgttg cccgtgtagt gcctttctgg gaaggtacca ttgtacctga
    26761 aatcaaaaaa ggccgcaaag tgattattgc tgcccacggt aacagcctca gggctttggt
    26821 gaaatacctt gacaacgttt cagaaaaaga catcctcgaa ctcaacatcc caacaggtac
    26881 accacttgtt tacgagctcg atgctaatat gaaacctatc aaacattact accttggcga
    26941 ccaggaagct atccgcaaag caatggaatc agttgctaac cagggaaaag caaagtaatt
    27001 ctttgaaaat aaataaaata aagccgggca gttttgtccg gttttttttg tcccaaaaat
    27061 cattccgtca acacttttcc accattcgtc actattttac ccttttttat ataaaatact
    27121 gataatcaat tgtttgtatt tttaacaaca tgaatacatt tgaataacaa attttaatca
    27181 ataactatta acgttcaaac tatgaaacga gcatgattcg ttaaacacaa acaaggatga
    27241 aaattcatca tgcgagtttt ccttcggaga gcccttcggg gttcattcgg ccttatttat
    27301 aaaattattt acggatgaaa gatagtataa atgtatctgc tgcttataaa ttgacaaaag
    27361 ccggaaaaag catatatagt agccaaaaac catgtattgg tgggtgataa aactattcct
    27421 gtagcccgca acaggaaaaa ggcttttttg aaaactatta tccgtggctc aaataccatt
    27481 catacagaaa aacataccac tcttttacca aaacctacca ttcaataaat aaatgttcac
    27541 atttgctttt tgcataaaaa agctatttat ctttgtttta accaatttga taaccatgaa
    27601 aacaatactg acattttttt taatatgcct attactttcc aatatttatg cacaaaagcc
    27661 ggtggctgat tttgagtttg
//
LOCUS       DAOSPF010000047        26601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1819_length_26601_cov_7.098433, whole genome shotgun sequence.
ACCESSION   DAOSPF010000047 DAOSPF010000000
VERSION     DAOSPF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_26601_cov_7.098433"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1382
                     /gene="tuf"
                     /locus_tag="PK252_09900"
     CDS             195..1382
                     /gene="tuf"
                     /locus_tag="PK252_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT15061.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITMVLAKKGLSEV
                     RSFDSIDNAPEEEERGITINTAHVEYQTANRHYAHVDCPGHADYIKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPKIVVFMNKVDLVEDAEMLDLVEMEMRELLNF
                     YNFEGDIAPVIRGSALGALNGDPKWEEKVMELMDAVDTFIPIPPRDTDKPFLMPVEDV
                     FSITGRGTVATGRIETGVVNTSDEMEIVGLGVEKKKTVITGVEMFRKILDRGEAGDNV
                     GLLLRGVDKEEIKRGMVIAKPGTITPHTVFKAEVYVLKKEEGGRHTPFHNKYRPQFYL
                     RTLDVTGEITLPEGREMVMPGDNVTITVTLIYPVAINKGLRFAIREGGRTVGAGQVIE
                     IIQ"
     gene            1431..1503
                     /locus_tag="PK252_09905"
     tRNA            1431..1503
                     /locus_tag="PK252_09905"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1464..1466,aa:Trp,seq:cca)
     gene            1520..1723
                     /gene="secE"
                     /locus_tag="PK252_09910"
     CDS             1520..1723
                     /gene="secE"
                     /locus_tag="PK252_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT15062.1"
                     /translation="MNFFKRIKNYISESYTELMHKVSWPTWNELQSSSIIVMIASVIF
                     ALIVLAMDLVFKYGVSGIYSIFS"
     gene            1741..2289
                     /gene="nusG"
                     /locus_tag="PK252_09915"
     CDS             1741..2289
                     /gene="nusG"
                     /locus_tag="PK252_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT15063.1"
                     /translation="MDQQKEKKWYVLRAVGGKEKKVKEYIDNEVNRLNLQNYITQVLI
                     PTEKVVQMRNGKRISKERIFFPGYVLIEAQLVGEIPHLLKDVPNVLGFLGSKGNEPTP
                     LRLSEVNRILGKVDELIEMGEEVALQFYVGEPVKVIDGPFNGFSGIIEEVNEEKKKLK
                     VMVKIFGRKTPLELNFMQVEKE"
     gene            2394..2837
                     /gene="rplK"
                     /locus_tag="PK252_09920"
     CDS             2394..2837
                     /gene="rplK"
                     /locus_tag="PK252_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT15064.1"
                     /translation="MAKEVVGLVKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQNMAGKVIPVVITVYADKSFDFITKTPPAAVQLIELSKVKGGSPEPNKNKVGSVTW
                     DQVKEIAQNKMPDLNCFTIESAMSMVAGTARSMGLTITGENPLKN"
     gene            2856..3551
                     /gene="rplA"
                     /locus_tag="PK252_09925"
     CDS             2856..3551
                     /gene="rplA"
                     /locus_tag="PK252_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT15065.1"
                     /translation="MAKLTKNRKLALSKVDTVKFYKLDEAAVLLKQITSTKFDASIDI
                     DVRLGVDPRKANQMVRGVVTLPNGTGKQTRVLVLCTPDKEQEAKEAGADYVGLDDYIE
                     KIKNGWTDVDVIITLPSVMAKIGALGRVLGPRGLMPNPKSGTVTMEVGKAVKEVKMGK
                     IDFKVDKFGIVHTSIGKVSFEADKIVENAREFLNTIMKLKPSSAKGTYVKSVYLSSTM
                     SPGLKIDTKLMTE"
     gene            3564..4085
                     /gene="rplJ"
                     /locus_tag="PK252_09930"
     CDS             3564..4085
                     /gene="rplJ"
                     /locus_tag="PK252_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT15066.1"
                     /translation="MRKEEKNTIIEDLVEQISANKHFYVADIANLNAATTSKLRRKCF
                     ENKIKLVVVKNTLLKKALEKIEGDYSEIYGVLNNSSSLLLCETGNAPAKLIKEFRKEC
                     EKPILKAAYVEGSVYLGDNQLNTLVSLKSREELIGDIIGLLQSPAKNVVSALQSGKNT
                     LAGVVKTLSERPE"
     gene            4136..4516
                     /gene="rplL"
                     /locus_tag="PK252_09935"
     CDS             4136..4516
                     /gene="rplL"
                     /locus_tag="PK252_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT15067.1"
                     /translation="MADLKAFAEQLVNLSVKEVNELAKILKDEYGIEPAAAAVAVAAA
                     PAAGGAAPAAEEKTQFDVILTSAGGAKLQIVKLVKDITGLGLKEAKDLVDATPKAVKE
                     KVSKEEAESIKAQLTEAGAEVEIK"
     gene            4623..8441
                     /gene="rpoB"
                     /locus_tag="PK252_09940"
     CDS             4623..8441
                     /gene="rpoB"
                     /locus_tag="PK252_09940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT15068.1"
                     /translation="MASKNIERISFASSKNRLAYPDFLEIQIKSFADFFQLETTPENR
                     KSEGLFKVFQENFPITDSRNNFVLEFIDYYIDPARYTIEECLERGLTYSLPLKAKLKL
                     YCNDPEHEDFDTVIQDVYLGTIPYMTPRGTFVINGAERVVVSQLHRSPGVFFAQNVHT
                     NGTKIYSARIIPFKGSWIEFTTDINNVMYAYIDRKKKLPVTTLLRAIGYESDKSILEI
                     FDLADEVKVSKSALKKLVGRRLAARVLKSWIEDFVDEDTGEVVSIERNEVVIDRETVL
                     ENDHIDLIVDANVDTILLHKEGTNMIDYAIIYNTLQKDPCNSEKDAVLHIYRQLRNSE
                     PPDEATARDVIDKLFFSDKRYDLGDVGRFKINRKLGLNIPSETKVLTKEDIISIIKHL
                     IELINSKTDVDDIDHLSNRRVRTVGEQLYNQFSVGLARMARTIRERMNVRDNEVFTPT
                     DLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRLSALGPGGLSRDRAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLINSLCVFAKINALGFIETPYRKVEAGKVDLSEEGV
                     MYMSAEEEEGKIIAHANAKLNNDGTFAEQRIKSRLEADYPQVSGEEVHLMDVAPNQIA
                     SIAASLIPFLEHDDANRALMGSNMMRQAVPLIRPESPIVGTGIEEQVITDSRIHIQAE
                     CDGVVEYVDANEIKIRMALTEDEKFVSFDEPVKTYRLPKFLKTNQSTCINLKPLVKKG
                     DKVEKGQILTEGYSAEKGELALGRNLMVAFMPWKGYNFEDAIVISERVVSEDMFTSIH
                     IDEYLLEVRDTKRGLEELTADIPNVSEEATRNLDENGIIRVGADVKPGDILIGKITPK
                     GETEPSPEEKLLRAIFGDKAGDVKDASLKATPSLDGTVIDTRLFSRAMKEKKGAKVIN
                     EKNLIQKIEDDFIRQTEELKAKMVDKLFIVVNGKTSQGVKDFLNVDVIPKGVKFSQKM
                     LQDLDFMNVNPSKWTTDKDKNQIIKDLFHNYILKYKEIDSIYKRKKHNLQIGDELPAG
                     IVQLAKVYIAKKRKVRVGDKMAGRHGNKGIVSRIVRREDMPFLEDGTPVDIVLNPLGV
                     PSRMNLGQIYETVLGWAGKELGVKFSTPIFDGASIEQINEYTQKAGIPNYGRTYLYDG
                     GTGEKFDQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEM
                     EVWALEAFGAANILQEILTIKSDDVIGRAKAYESIVKGEPMPVPGIPESLNVLLHELR
                     GLCLSINMTN"
     gene            8496..12788
                     /gene="rpoC"
                     /locus_tag="PK252_09945"
     CDS             8496..12788
                     /gene="rpoC"
                     /locus_tag="PK252_09945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT15069.1"
                     /translation="MSFRKDNKQKSSFSKIAIGLASPEEILEHSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIN
                     LVVPVAHIWYFRSIPNKIGYLLGIPSKKLDSIIYYERYVVVNAGIKAADGIKEMDLLT
                     EEEYLDILETLPKENQYLDDSDPSKFIAKMGAEGIHMLLEKIELDNLSYSLRNQASKE
                     TSQQRKSEALKRLQVVEAFRESKETNKPEWMICKVIPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKASAVKTDANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKLHECGLPKDMAAELYKPF
                     IVRKLLERGIVKTVKSAKKIVERKDAVIWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLVCTAFNADFDGDQMAVHLPLGNAAILEAQILMLASHNILNPANG
                     APITVPSQDMVLGLYYMTKPRMSTPEHKVRGEGMTFYSSEEIIIAFNEKRVDLHAKIK
                     IKNNYTDEPDRKFIETTIGRVLFNEVVPREVGYINEVLTKKSLRDIISKVLKVTGTSR
                     TAKFLDDIKELGFKMAFTGGLSFNLADVIVPEEKDKLVEAGYKEVDEIISQYNNGFIT
                     YNERYNKVIDNWTHTNSKLTDILMKKISTHNQGFNPVYMMLDSGARGSKEQIRQLAGM
                     RGLMAKPQKSGSTESQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGY
                     LTRRLVDVSQDVIITETDCGTLRGLEASAIKKSEEIVESLYERILGRTSVHDIYHPQT
                     GELLVGAGEEFTEEIAAKISESPIDTVEIRSVLTCESKKGVCAKCYGRNLATGRMVQI
                     GEAVGVIAAQSIGEPGTQLTLRTFHVGGVASNIATESDLIAKYDGIIEIDELRTVEGI
                     RDGEKVDIVIGRMAEVRLVDKKTGSVLTTNSIPYGSKLFVKNGAEITKGTRICEWDPF
                     NALIISEVSGKIAFDSVIDGVTFKEESDEQSGYKEKVIIESRDKTKNPMIKVISNDSD
                     IVRSYNLPVTSHVIVEEGDTIKAGDIIAKIPRNVGKSGDITGGLPRVTELFEARNPSN
                     PAVVAEIDGEVTFGKIKRGNREIKITSKTGEVKNYLVSMSKQILVQENDYVKAGTPLS
                     DGAITPSDILAIKGPTAVQEYIVNEIQEVYRLQGVKINDKHFEVIVRQMMRKVEIIDP
                     GDTKFLEKQIVDKLEFMAENDELFGKKVVVDAGDSTTLKAGQIITARKHRDENSLLKR
                     RDLRQVEARDALPATSSQILQGITRAALQTQSWFSAASFQETTKVLNEAAIFGKVDTL
                     EGLKENVIVGHQIPAGTGLREYQNIIVGSAEEYARLVTSSDSSLDEVEVDEE"
     gene            12909..13223
                     /locus_tag="PK252_09950"
                     /pseudo
     CDS             12909..13223
                     /locus_tag="PK252_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
     gene            13258..14316
                     /gene="recA"
                     /locus_tag="PK252_09955"
     CDS             13258..14316
                     /gene="recA"
                     /locus_tag="PK252_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT15070.1"
                     /translation="MAQNTDVEQTKKEINKEKLKALQLTIDKIDKDYGKGTIMRMGEK
                     AALDVPVISSGSIGLDYALGVGGYPRGRVIEIYGPESSGKTTLAIHAMAEAQKAGGIA
                     AFIDAEHAFDRFYAEKLGVDIENLLISQPDNGEQALEIADALIRSGAIDIIVIDSVAA
                     LTPKAEIEGDMGDSKLGLQARLMSQALRKLTANINKTNTCCIFINQLREKIGVMFGNP
                     ETTTGGNALKFYASVRIDIRKNQQIKDGEEILGVRTRVKIVKNKLAPPFKKADFDIMY
                     GEGISKSGEIVDLGVELEIIKKSGSWFSYGDTKLGQGRDAVKNLLRDNPELMLELEQK
                     IFKALQTGGKVLFTDKAN"
     gene            14336..15916
                     /locus_tag="PK252_09960"
     CDS             14336..15916
                     /locus_tag="PK252_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT15071.1"
                     /translation="MNSYATYKAYSHEVYFWLLAFIAISMPYMELFSIIGLILLFINW
                     ILEGNFSHKLSIFKHKKSISVFSAIFFIHVIWLLGTSQWDEAFRNIFIKLPLLILPVI
                     IGTSEPIKIPKVRKLLQLFIGSVSLAVVAHFIISKKWLPVDSDKILKSGIFVSKSQLT
                     CMVVVSTLTLFHWIKSMGRNFKASLVVYIVLAILMLVYLASIHSLAGLIVLAITFLIL
                     VISYINRLENQMLKIGLIMLIYAIPFFSIVLMIKTYHQFFHSKQPEYNVSQLFTKAGN
                     PYYNQSASWEIENGNKVWVNVCEKELSKEWNKISEIKYEDLDLKGQPLRLTLIRYMTS
                     KGLKKDAEGIALLSGRDIINVQKGMTNYIFDTHWSLLTRIYEVFWEVHQFKHSKCSSQ
                     EHSLSVRLEKYHIALSVAAKHLFFGVGTGDLDLIYSDLNKGHKICLKAEDKNDTGNQF
                     LYVLCSFGIIGLILFIFALVLPGILEMKWDSYYFLMIFCVIVLSFFVFEVLESFAGTI
                     FCTFFYSFYLWGTYRNLE"
     gene            15918..16430
                     /locus_tag="PK252_09965"
     CDS             15918..16430
                     /locus_tag="PK252_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOT15072.1"
                     /translation="MNRLDDAFFQRDVLDLAPLLLGKTIVRKFDDSSESRFLITETEA
                     YKGEEDLACHASKGRTPRTEVMYQPGGCLYVYLIYGMYWMLNIVTGEKDNPQAVLIRG
                     LDAVYGPGRLTKKLQIDKSFNGERLQTSERIYLLNNDLKCDYSVAPRVGIDYAGEYWR
                     DVPWRFTLKL"
     gene            complement(16414..17526)
                     /locus_tag="PK252_09970"
     CDS             complement(16414..17526)
                     /locus_tag="PK252_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15073.1"
                     /translation="MKKLVHIAFIFSSLLLNGQENIQDSSLNSKANLAFHFQNANFVK
                     DNEYFNDVVEGYTLIGLWATPTISYNITDKATIEGGLHALKYSGQDNFNKILPVYRID
                     YRITPTLCFTLGTLHFNDNHLQPAPMLDPERFYLGQADNGVQFMLNKQNLKGDLWLSW
                     DKFTWYGDSVQEKLTSGLALNFNIPVAETFSVKIPANLIATHLGGQINRPKKPIETLI
                     NFSSGLFASFKIDLGIFKDLQIGSRAFLYNKNTQVETRPFKSGYALLPEISVNSGLLD
                     ASFSYWYSHKFIGPRGEAMYQCISTQPERKDTLYNTRKIGIIKVNYTKEIVNALSFKC
                     GFEGYQILDQKLFDYNFSLSFICQIDKILPRPKASK"
     gene            complement(17536..17844)
                     /locus_tag="PK252_09975"
     CDS             complement(17536..17844)
                     /locus_tag="PK252_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15074.1"
                     /translation="MNNAINDIQTQINSIAVYNDENLKQYQQLLKLGMDRLQYLQKEG
                     LTPMSEIWVNMAIDEIKKELETRFNANFSALSPDRQKTEFLYSRSAVSLVLTNVLMHL
                     "
     gene            complement(17938..18933)
                     /locus_tag="PK252_09980"
     CDS             complement(17938..18933)
                     /locus_tag="PK252_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOT15075.1"
                     /translation="MSKTIIGQNLPNIPWEEKPAGLHLPIWRYSKNPIIDWNPTPTTA
                     RIYNSAVLPHNGEFIGVFRADHKNGRAFLHFGRSKDGINFDIDDNVIPWQDENGKPNL
                     VSYAYDPRFVKIDDTFYVTWCDDMHGASIGMGYTKDLKTWVRLPNPLMPFNRNGVLFP
                     RKINGKYKLLSRPSDSGHTPFGDIFISESPDLVYWGNHKWVMGKGGKGWWQGTKIGAG
                     PVPIETSEGWLLFYHGVSGTCNGFVYSFSAVILDINDPSIVKYRCGDYLMTPEKPYET
                     TGFVPNVLFPCQNIYDAETGRIAIYYGAADTYTAIAFCQVDEIIDYVKKHHEDVK"
     gene            complement(18966..21356)
                     /locus_tag="PK252_09985"
     CDS             complement(18966..21356)
                     /locus_tag="PK252_09985"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15076.1"
                     /translation="MNYGYFDDKRKEYVITRPDTPLSWSNYLGSTTYGAIITNNAGGY
                     SFYKSAAQGRFMRYRTNTIPMDQPGRYIYVHDCESKDFWSTSWQPVGKPLDKFKSECR
                     HGSAYTIIESEYAEILTNTRYFVPLGSEYEYWQVKVSNKSNRKRKIRLFTYVEFASHW
                     VLSQDIINLQYTQYVLKMDVVNNIISAGTNVNLPHIPEEFENDRQSRYSFIGVAGATP
                     TRFDTDRKAFLGTYRSYHNPVVVENGQCTGSCKAGDNGCGTIQIDIELEAGETKEFVV
                     VLGVGKADTVGIKAIKEASDINAVNKKFDDLVNFWHSRLEGMHVKSPDALFDSMFNMW
                     NPYNSMMTFTWSRAASLIYAGERDGLGYRDTVQDILGVIHLVPEEARQRLELMITGQV
                     STGGAMPVVKQFGHYPGQMPKTEETEYRSDDCMWLFNTIPAYVKETGDINFYRKTLPF
                     ADQGEATVLGHLRRAIEFNLERSGAHGLPCGLVADWNDCLVLGHKGETVFVALQLRYA
                     LKEYAEICELLGEAAEKKWALEKLAILDENIEKHAWDGRWYLRAYREDGLKFGTDGDE
                     EGSLWLNPQSWAVLSGHANADRSIKVMDIVKERLATDYGTMICDPPYKKADLNVIKAT
                     LFNPGSKENGAIFCHTQGWVVLAETLIGNGNRAFEYYKAYLPASCNNKAEIREIEPYV
                     YSQSTHSKYHDRYGASRLPWLTGAATWAYFAASQGILGIKPDYNGMVIDPCIPEGWGE
                     FSVVRRFRGKKLNITVKNPDKKQKGVKQLMVNGEKLGNNFIPVEKLNAENQIEVTL"
     gene            complement(21436..22944)
                     /locus_tag="PK252_09990"
     CDS             complement(21436..22944)
                     /locus_tag="PK252_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOT15077.1"
                     /translation="MESTHTKLKFREKVGYGFGDLGSVLFWQTIMVYLLYFYTDVFGL
                     TAATAGVMFFVSRVLDAFFDVVIGMTADRTETRWGKFRPYILFGAVPLAVAAVAAFTV
                     PSFGGTGKIVYAYITFLSFMFLYSTVNIPYTSLLGVISNDAVDRTSASSFKFIGAYLA
                     GIIVSATALPFAKMFGKGGTELEIAQSGWHTTMLIYAIAAVLFFFITFLSTKERIKPI
                     AKEKTNIKSDLKDISKNIPWMVLFAITILFILFNCIRLNVTTHYFKYYVGEQSVPWLT
                     DILNFFRGYIINPIISLFGNEKLTVLNRNETFGFEALTSIFNTVGQTLSLIGVILVPW
                     FAKTFGRKEATIILFVTALICTGSFIFFSPENIFLILFFQAIGSITGGPISALLWVMY
                     ADTADYSEWKTGRRATGLIFSASIMSNKLGWAFGSMIAAFILASTGFVANVTQNAGTL
                     NGLKSMMSVIPVIPGLLALTLLIFFYKLDEKTMKKVQEDLNQRRIESGSSAI"
     gene            23306..24052
                     /locus_tag="PK252_09995"
     CDS             23306..24052
                     /locus_tag="PK252_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15078.1"
                     /translation="MPYRRLPNTDVARIKAMKIAYNKAKELPPFKLAFSQSLFQRLQS
                     FMPLFEKNVLESKQTYANKVNKSKDYQQALRKARLYISHFLQVMNMAIQRGEQQPAIR
                     EFFGIDPNNSRLPNLISEESVLEWGEKLIKGEQERIKRGMTPITNPTIAVVKVRYEQF
                     KETYHAHKIMQKSSSRYVNELAQLRKTADEIIALIWDDVEKTFANLPEELRREKCNEY
                     GIIYVFRKNELSRISLPFVKAAPSLFDSIL"
     gene            complement(23968..24636)
                     /locus_tag="PK252_10000"
     CDS             complement(23968..24636)
                     /locus_tag="PK252_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT15079.1"
                     /translation="MCHYRLMLRYFVIYKPYNMLSQFTPEGEHQTLKDLDFTFPKDVY
                     PVGRLDADSEGLLLLTNDTAINKLLLNPKNQHPRKYLVQVEGAIDAEAIKMLESGLEI
                     NNNGKPYMTQPAKARIIDIEPLIPPRVPPVRFRKTVPTSWIELTLIEGKNRQVRKMTA
                     KAGFPTLRLLRIAIGNIKLDNMQPGEVKEIDKEKVYNILSNKLGAALTKGKDIRLNSF
                     LRKT"
     gene            24674..24949
                     /locus_tag="PK252_10005"
     CDS             24674..24949
                     /locus_tag="PK252_10005"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HOT15080.1"
                     /translation="MHDGTIIEANKNIFFNRIDDEIVMMDEAQGKYYSLNPVASRIFE
                     IVQQKCSFRQLLDQLLSEYEITEEECKTQTIVFLNDLKSKNLIFIDE"
     gene            24942..25382
                     /locus_tag="PK252_10010"
     CDS             24942..25382
                     /locus_tag="PK252_10010"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HOT15081.1"
                     /translation="MSKSKLHNFFRLSATDMFYFFVALYFSVLYWICTRLLPYSVWKN
                     ILGTATKTGTPSLKEQEKSKFRTVIIAVNRCSRHVPFARNCLIKALTTRKILRMKGIK
                     STLCLGLNNENKKLLTAHAWVKIGPDLTIGESKNENFIVVVMYK"
     gene            25514..25825
                     /locus_tag="PK252_10015"
     CDS             25514..25825
                     /locus_tag="PK252_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT15082.1"
                     /translation="MNLIELNRDRLFELCDSHKVKELYLFGSVLTEKFNDSSDIDMLI
                     QFSQVELVYYFDNYMDLKEKLEDLFNRPVDLVENQAIRNPVFRQIIDRDKHLVYERKS
                     A"
     gene            25806..26141
                     /locus_tag="PK252_10020"
     CDS             25806..26141
                     /locus_tag="PK252_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT15083.1"
                     /translation="MNEKVLKSLYDIKLAIEEIDTFFSTRDKRFDDYTNDLLLKRAIE
                     RNLEIIGEAMNRILKEDPQISIDNAKRIVGLRNQIIHGYDSVSDENIWGIVINHLPKL
                     KDEISKLIQ"
BASE COUNT         8485 a         4584 c         5501 g         8031 t
ORIGIN      
        1 tcaggggtag agcacttcct tggtaaggaa ggggtcatgg gttcaattcc catcaatggc
       61 tctaaaaatc aatcagatag atttgccatt atttgattga ttattttgat ttttgatgta
      121 ttgaataaat tcgataataa cgtttatttt tttgaaaata attataaatt aataatcttc
      181 aacaattttg agttatggca aaagaaaaat ttgacaggtc aaaacctcac gttaatattg
      241 gaaccattgg tcacgttgac catggaaaga caaccttgac cgctgcaatt actatggtgc
      301 ttgcaaaaaa agggttatct gaagttcgtt ctttcgattc tatagacaac gctcctgaag
      361 aagaagaacg tggtattaca attaatacag ctcacgttga atatcagaca gctaaccgtc
      421 actacgcaca cgtagattgc cctggtcacg ctgactatat taagaacatg gtaactggtg
      481 cagctcagat ggacggtgct attatcgttg tagcagcaac tgatggccct atgcctcaga
      541 cccgtgaaca tattcttctt gctcgtcagg taaatgttcc taaaattgta gttttcatga
      601 acaaagttga cttggttgaa gatgcagaaa tgcttgacct tgttgaaatg gaaatgcgtg
      661 aacttttgaa tttctataat tttgaaggag atattgctcc tgttattcgc ggttctgctt
      721 taggtgctct taatggagat cctaagtggg aagaaaaagt tatggaattg atggatgcag
      781 ttgatacctt tattccaatt cctccacgcg acacagacaa accattcctt atgccggtag
      841 aagacgtatt ctctatcact ggtcgtggta ctgtagctac aggtcgtatc gaaactggtg
      901 ttgttaacac atcagacgaa atggaaattg ttggtcttgg tgttgagaag aagaaaacag
      961 ttattaccgg tgttgaaatg ttccgtaaaa ttttggatcg tggagaagct ggtgacaacg
     1021 taggtttatt gcttcgtggt gttgataaag aagaaattaa acgtggtatg gttattgcaa
     1081 aacctggtac cattactcct catactgttt tcaaagccga agtttacgtt ttgaaaaaag
     1141 aagaaggagg acgtcacact ccattccaca ataaatatcg tcctcaattt tacttacgta
     1201 cccttgacgt aactggagaa attactttac cagaaggacg cgagatggtt atgcctggtg
     1261 acaacgttac tattacagta acattgattt accctgtggc tattaacaaa ggtttacgat
     1321 ttgcaattcg tgaaggtggc cgtactgttg gtgcaggaca ggtaattgaa ataatacaat
     1381 aatatgcaag ataaaaacca agggttatta ttaactcttg gttttaacat acgggtgtag
     1441 ctcagttggt agagcattgg tctccaaaac caagtgtcgg gcgttcgagt cgctcctccc
     1501 gtgcttcatt tattaacata tgaatttttt taagagaata aagaattaca ttagtgaatc
     1561 atacactgag cttatgcaca aagtttcatg gcctacatgg aacgaattgc aaagtagtag
     1621 catcatagta atgatagctt cagtgatatt tgcactgatt gtattagcta tggacttggt
     1681 atttaaatat ggggttagtg gcatttatag tattttttct taagctggag cacttgataa
     1741 atggaccaac aaaaagaaaa aaaatggtat gtccttagag ctgtaggcgg taaggaaaaa
     1801 aaagttaagg aatacattga taatgaagtc aatcgactta acttacaaaa ttatatcact
     1861 caggtattaa tacctacaga gaaagttgtg cagatgcgaa atgggaaacg cattagtaaa
     1921 gaacgcatct tttttcctgg gtatgtatta atagaggctc aattggttgg tgaaatacct
     1981 catttattaa aagatgtgcc aaatgtattg ggtttcttag gaagtaaagg taatgagcct
     2041 acccctttga ggctgagtga agtaaacagg atattaggaa aagttgatga actcattgaa
     2101 atgggtgaag aagtagcttt acagttttat gttggagaac ctgttaaagt tattgatggg
     2161 ccattcaatg gtttctctgg tattattgaa gaagtaaacg aagaaaagaa gaaactcaaa
     2221 gtaatggtta aaatttttgg acgaaaaaca ccattagagt tgaacttcat gcaagtagaa
     2281 aaagaataaa aactttgact ttaccagttt aagtattacg aagaatcctc tcgtaaataa
     2341 tgcttccctt tgtaagaaag caaacaggta gtcaatatta atgttaatat attatggcaa
     2401 aagaagtagt tggattagta aaattgcaaa ttaagggagg ggcagccaat ccttcgccac
     2461 cagtagggcc ggcattaggc tcgaaaggta tcaatattat ggagttttgc aagcaattta
     2521 atgcaagaac ccaaaatatg gcagggaagg ttataccagt tgttattact gtgtatgccg
     2581 ataagtcatt tgactttatt accaaaaccc ctccggcagc tgttcagctt attgaacttt
     2641 caaaagtaaa aggcggttct cctgagccaa acaaaaataa ggttgggtct gttacctggg
     2701 atcaggttaa agaaatagca caaaataaaa tgcctgacct taattgtttt acaattgaga
     2761 gcgctatgag tatggttgct ggtacagcac gtagcatggg actcaccata actggtgaaa
     2821 atccgttaaa aaattaataa aaataagacg aataaatggc taaactgacg aaaaacagaa
     2881 aattagctct gtctaaagtt gatacagtta agttttataa gttagatgaa gctgcagttt
     2941 tgttgaagca aattacttct acaaaatttg atgcttcaat agacattgat gtaagacttg
     3001 gtgtagatcc tagaaaagct aatcagatgg ttcgtggtgt cgttacattg cccaatggaa
     3061 caggcaaaca aaccagagtt ttagtactct gtacacccga taaagaacaa gaagcaaagg
     3121 aagccggggc agactatgtt ggtcttgacg attatatcga aaagattaaa aatggatgga
     3181 cagatgtaga tgtgattatt acattacctt ctgtaatggc caaaatagga gctctcggtc
     3241 gtgtattagg ccctcgtggg cttatgccaa atcctaagag tggtacagtt accatggaag
     3301 ttggaaaagc tgtaaaagaa gtaaaaatgg gtaaaatcga ctttaaagtt gataaattcg
     3361 gtatagtaca tacttcaatt ggaaaagttt cttttgaagc tgataaaatt gtagagaatg
     3421 ctcgtgaatt cctaaatact atcatgaagc ttaaaccttc ttctgcaaaa ggtacttatg
     3481 taaaaagtgt ttacctttct tcaacgatga gcccgggatt gaagattgat acaaaattaa
     3541 tgaccgagta aacttttgat gttatgagga aagaagaaaa aaacacgatt atcgaagatt
     3601 tagttgagca aatttcagcc aataaacatt tttatgtagc agatattgca aatcttaatg
     3661 ccgctacgac tagcaaatta agacgtaaat gttttgaaaa taagattaag cttgtagttg
     3721 taaaaaatac tttgcttaaa aaggcgctcg aaaaaattga aggagattac tctgaaattt
     3781 atggggtttt aaacaattca tcttctttat tactttgcga aacaggaaat gctcctgcca
     3841 agcttattaa agaatttaga aaagaatgtg aaaaacctat tcttaaagct gcgtatgttg
     3901 aaggtagcgt ttacttagga gataatcaat taaataccct tgtaagcctt aaatccagag
     3961 aagaacttat tggcgacatc attgggttgt tacaatctcc agctaaaaac gttgtttcag
     4021 cattacagtc aggaaagaat actcttgcag gtgtagttaa gacattgtca gaacgccctg
     4081 aataacaaat taaaaacaaa taaaaaaagt aataacattt taaatttcta taaaaatggc
     4141 agatttaaaa gctttcgcag aacaacttgt taacctttca gttaaagaag ttaatgaact
     4201 tgcaaaaatc ttaaaagacg aatatggaat tgaacctgct gcagctgcag tagctgtagc
     4261 tgcagctcca gcagctggtg gtgctgctcc agcagctgaa gaaaaaacac aattcgatgt
     4321 tattttaacc agtgccggtg gtgctaaatt acaaatagta aaattggtta aagacatcac
     4381 aggacttggc ttaaaagaag ctaaagactt agttgatgct acaccaaaag cagtaaaaga
     4441 aaaagtatca aaagaagaag cagaatcaat taaagcacaa ttaacagaag ccggagccga
     4501 agttgaaatt aaataatatc ttttgatata tcatacatgg gtttagggac catgcaaaag
     4561 catggatttc cctaagcctt tttgtcgttt tattcataga gttaatttaa tccaatctat
     4621 caatggcttc aaagaatatt gaaagaataa gttttgcttc ttcaaaaaat cggttagcgt
     4681 atcccgattt tcttgagatc caaataaaat catttgctga tttctttcag ctcgagacaa
     4741 ctccagagaa cagaaagagc gaaggtttat ttaaagtgtt ccaggaaaat tttcctatta
     4801 cagactcccg gaacaacttt gttttagaat ttatcgatta ttatattgat cctgccagat
     4861 atactatcga agagtgtctt gaaagaggct tgacctatag tcttcctttg aaagctaaac
     4921 tcaaattgta ttgcaacgat cctgaacacg aagattttga cacagttatt caggacgtat
     4981 atcttggaac aattccatac atgactccac gagggacttt tgtcatcaat ggcgctgaac
     5041 gtgttgttgt gtcacaatta cacaggtctc cgggtgtatt ttttgctcaa aatgtacata
     5101 ccaatggtac aaaaatctat tctgcaagaa ttattccttt caaaggttcg tggatagaat
     5161 ttactactga catcaataac gtaatgtatg catacattga caggaagaaa aagcttcctg
     5221 ttactacatt gttacgtgca attgggtatg aaagtgacaa gagtattctt gaaatttttg
     5281 accttgctga tgaagtaaaa gtttcaaaaa gtgcccttaa aaaacttgtt ggaagacgtc
     5341 ttgctgcaag agttttgaag tcatggattg aagactttgt tgatgaagat accggtgaag
     5401 tagtttcaat cgagcgtaat gaagttgtta ttgaccgtga aacagttttg gaaaacgatc
     5461 atatcgactt aattgttgac gcaaatgttg ataccattct tttgcataaa gaaggtacca
     5521 atatgattga ttatgctata atatataaca ctttacaaaa agacccttgt aactcagaaa
     5581 aggatgctgt acttcatata tacagacagt tgcgaaacag tgaacctccc gatgaagcta
     5641 ctgcccgtga tgtgattgat aagttattct tttcagacaa acgttacgat ttaggagatg
     5701 ttggccgttt caaaatcaac cgtaaacttg gcttaaatat ccctagtgaa actaaagttc
     5761 ttactaaaga agacattatt tcaatcataa aacatcttat tgagcttatc aactctaaga
     5821 cagatgttga tgatatagac cacttaagta accgtagggt caggactgtt ggagagcaat
     5881 tgtacaacca gtttagtgtt ggtttagctc gtatggcacg tactatcaga gaaagaatga
     5941 acgtacgcga caacgaagta tttaccccaa ctgacctgat taatgctaaa acattatctt
     6001 cggttatcaa ttcattcttc ggaaccaacc aattatccca gttcatggac caaaccaacc
     6061 cactggcaga aatgacacac aaacgtcgtt tgtctgcatt gggaccagga ggtctttcgc
     6121 gagacagggc aggttttgag gttcgtgacg ttcactacac tcactatgga cgtctttgcc
     6181 ctattgagac tcctgaaggg ccaaacattg gtcttatcaa ctcactttgc gtttttgcaa
     6241 aaattaacgc tttaggtttc attgaaacac cttatcgtaa agtagaagcc ggaaaggtag
     6301 atctttctga agaaggtgta atgtacatga gcgcagaaga agaagaaggt aaaataatag
     6361 ctcatgcaaa tgctaagctc aacaacgatg ggacatttgc tgaacaacgt attaagtcaa
     6421 ggcttgaagc tgactatcct caggtatccg gcgaagaagt tcatttgatg gacgttgccc
     6481 caaaccagat tgcttctatt gctgcttcac ttattccatt tcttgagcac gatgatgcaa
     6541 accgtgcgtt gatggggtca aacatgatgc gtcaggcagt gcctctgatc aggcccgaat
     6601 ctcctattgt tggtacaggt attgaagagc aggttattac agactctcgt attcatatcc
     6661 aggctgaatg tgatggggtt gttgagtatg ttgatgctaa cgaaataaaa attcgtatgg
     6721 cccttacaga agatgagaag tttgtaagtt ttgatgagcc agtaaagaca taccgtttgc
     6781 ctaaattttt aaagacaaac caaagtactt gtatcaactt aaaaccactt gttaaaaaag
     6841 gagataaagt tgagaaaggt caaatactaa cagaagggta ttcagccgaa aaaggagaac
     6901 ttgcgcttgg ccgaaacttg atggtagcat tcatgccatg gaaaggttat aattttgagg
     6961 atgctattgt tatcagcgaa agggtagtga gtgaagacat gttcacttca atccatatag
     7021 atgaatactt actcgaggtt cgtgatacaa aacgaggttt ggaggaactt actgctgata
     7081 taccgaatgt aagtgaagaa gcaacccgta accttgatga gaatggaatt attcgtgtag
     7141 gtgcagatgt aaagcccgga gatattctta ttggtaaaat aactcctaaa ggagagacag
     7201 aaccttcacc agaagaaaaa ctgcttaggg ctatttttgg tgataaagct ggtgatgtaa
     7261 aagatgcttc tttaaaggca actccttctc ttgatggaac tgtcatagat acaaggctct
     7321 tttcaagagc tatgaaagaa aagaaagggg ctaaagttat caatgaaaag aaccttattc
     7381 aaaaaattga agatgatttc ataagacaaa cagaagagtt gaaagccaag atggtagata
     7441 aacttttcat tgttgtaaat ggaaaaactt cgcaaggagt taaggatttc ctgaacgttg
     7501 atgttattcc gaaaggagta aaatttagcc agaagatgtt acaggatctt gattttatga
     7561 atgttaaccc aagtaagtgg acaacagata aagacaagaa ccaaatcatt aaagatcttt
     7621 tccataatta tattttaaaa tataaggaaa tagatagcat atataagcgc aaaaagcaca
     7681 atttgcagat tggagatgaa ttgcctgccg gaattgttca actagctaaa gtttatattg
     7741 ctaaaaagag aaaagtgcga gtaggggaca aaatggcagg gcgtcacggt aacaaaggga
     7801 ttgtatctcg tattgtacgt cgtgaagata tgccatttct tgaagatggt actcctgtag
     7861 atattgtatt gaacccgctt ggtgtgcctt ctcgtatgaa tcttggacag atatatgaaa
     7921 ctgtacttgg ttgggcaggg aaagagcttg gagttaagtt ctctacacca atttttgatg
     7981 gagcttcaat tgaacaaatt aatgaatata cgcaaaaagc cggaatccct aattatggta
     8041 gaacatactt atacgatgga ggtacaggtg agaagttcga tcagcctgca acagtaggtg
     8101 ttatatacat gctgaaactt ggtcacatgg ttgacgataa gatgcatgct cgttcaattg
     8161 ggccatattc acttattaca caacaacctc ttggtggtaa agcacaattt gggggccagc
     8221 gttttggtga aatggaggtt tgggcgcttg aagcattcgg tgctgctaat atattgcagg
     8281 aaattctgac aattaagtca gacgatgtaa taggcagggc taaagcatac gaatcaattg
     8341 ttaaaggaga gccaatgcct gtccctggta ttcctgagtc attgaacgtg ttgctgcatg
     8401 agctcagagg tttgtgtttg agtatcaaca tgaccaatta atattgggaa acattgaatg
     8461 tagagtcttt ttagtaaatg aaaaaatagc gacgtatgtc attcagaaaa gataataaac
     8521 aaaaaagcag cttttcaaag atagctatag gtttggcttc tcccgaagaa attcttgaac
     8581 attccagcgg tgaagtactt aagccagaaa ctatcaatta tcgtacttat aaaccagagc
     8641 gagacggttt attttgcgaa cgtatttttg gaccagttaa agattatgaa tgccattgcg
     8701 gcaaatacaa gcgaatcagg tataaaggga ttgtttgcga tcgttgcggt gttgaagtaa
     8761 ctgagaaaaa agtacgtcgc gaacgtatgg ggcatattaa tcttgtggtt cctgttgccc
     8821 atatttggta tttcaggtca attcctaaca aaattggtta tttacttggt attccaagca
     8881 aaaagcttga ctctattata tactatgaaa gatatgttgt agtaaatgct ggtattaaag
     8941 ctgccgacgg aattaaggaa atggatttgc ttaccgaaga agaatatctt gatattttag
     9001 aaactcttcc taaggaaaac caatatcttg atgatagtga cccaagtaaa tttattgcaa
     9061 aaatgggcgc tgaaggtatt catatgcttc ttgaaaaaat agaattagac aatctttcat
     9121 attcacttcg taaccaggct agcaaagaaa catcacaaca acgtaaaagt gaggctttga
     9181 aaaggcttca ggttgttgaa gctttccgtg aatctaaaga aaccaacaag cctgagtgga
     9241 tgatttgcaa ggttattcca gtaattcctc cagaattaag gcctttggtc cctctcgatg
     9301 ggggacgttt tgctacgtca gatctcaacg acctttacag aagggttatt atccgaaaca
     9361 accgtttaaa aaggctttta gaaatcaaag ctccagaagt aattttacgt aacgaaaaac
     9421 gtatgttaca ggaagctgtt gactcattgt ttgacaactc tcgtaaagca agtgctgtta
     9481 aaactgatgc taacaggcct ttgaaatcat tatcagacag cttaaaaggg aaacaaggcc
     9541 gttttcgtca aaatttactt ggtaaacgtg ttgactattc tgcacgttcg gtaattgttg
     9601 taggtccaga gcttaagttg cacgaatgtg gtttgcccaa agatatggct gcagagcttt
     9661 acaaaccttt tattgtaagg aaacttcttg aaagaggtat tgtaaagact gtaaaatcag
     9721 ctaaaaaaat tgtagaaaga aaagatgctg ttatttggga tatccttgaa aacgttttga
     9781 agggacaccc ggtattgttg aaccgggccc cgactcttca ccgtttgggt attcaggctt
     9841 ttcagcctaa acttattgaa ggtaaagcta ttcaattgca cccactcgtt tgtactgcgt
     9901 tcaacgctga ctttgatggt gaccaaatgg ctgtacacct gccattaggc aatgcagcta
     9961 ttcttgaagc tcaaatattg atgttagctt cccacaatat ccttaacccc gcaaatggtg
    10021 caccaatcac ggtcccctct caggacatgg ttttagggct ttattatatg accaaaccac
    10081 gtatgagtac cccagaacac aaggtgcgtg gtgaaggtat gacattctat tcatcagaag
    10141 aaattatcat agcattcaat gagaaacgtg ttgatttgca tgctaagatt aaaatcaaaa
    10201 ataactatac ggacgaaccc gacaggaagt ttattgaaac taccattggc agggtacttt
    10261 tcaacgaagt agtccctcgc gaggtgggtt atattaacga agttcttact aaaaagtcac
    10321 ttcgagatat tatcagcaag gtacttaaag ttacaggtac atcccgtact gctaagtttc
    10381 ttgatgatat taaagaactt gggtttaaga tggcttttac cggaggcctt tctttcaacc
    10441 ttgccgatgt aattgttcct gaagaaaaag ataaacttgt agaagctggt tataaagaag
    10501 ttgacgaaat cattagccaa tacaacaatg gtttcattac ttataacgaa cgttacaata
    10561 aagttattga taactggact cacacaaaca gcaagctgac tgatattctt atgaagaaaa
    10621 tcagtacaca caatcagggc ttcaatcctg tttacatgat gcttgattct ggagctcgtg
    10681 gttctaaaga gcagattcgt cagttggctg gtatgcgtgg tcttatggca aaacctcaga
    10741 aatcaggttc tacagaaagc cagattattg aaaaccctat tctttctaac tttaaagaag
    10801 gattgtcagt acttgagtac tttatttcta ctcacggagc tcgtaaaggt ttggctgata
    10861 ctgctttgaa aactgcagat gcaggttacc ttacccgacg tttggttgat gtatcgcaag
    10921 atgtgattat aacagaaaca gactgtggaa cattgagggg gcttgaagct tctgctatta
    10981 agaaaagtga agaaattgtt gagtctttgt acgaaagaat tcttgggcga acctcggttc
    11041 atgatattta tcacccacag actggcgaat tgcttgttgg tgccggagaa gaatttacag
    11101 aagagattgc tgccaagatt tcagaatcac ctatagatac tgttgaaatc cgttcagtgt
    11161 tgacctgtga atctaagaaa ggggtatgtg caaaatgtta cggacgaaat ttagcgacag
    11221 ggcgcatggt gcaaattggc gaagcggtag gagtaattgc cgcacaatca attggcgagc
    11281 ctggtacaca gcttacactt cgaacatttc acgttggtgg tgttgcgtct aatattgcca
    11341 cagaatcgga tttaattgct aaatacgatg gtattattga gatagatgaa ttgcgtacag
    11401 ttgaagggat aagagatggt gaaaaagttg atattgttat tggacgtatg gcagaagtac
    11461 gtttggtgga caaaaagacg ggtagtgtcc ttacaactaa tagtattcct tatggttcaa
    11521 aactatttgt aaagaatggt gctgagatta caaaagggac acgtatttgt gagtgggatc
    11581 cattcaacgc gcttatcatt tctgaagtta gtggtaagat agccttcgat tcggttattg
    11641 atggtgttac atttaaagaa gaatctgacg agcaaagtgg atataaagag aaagtaatta
    11701 ttgagtcaag ggacaagact aaaaacccta tgattaaagt aattagcaac gattcagaca
    11761 ttgttcggtc ttataacttg cctgttactt ctcatgtaat tgttgaagaa ggagatacaa
    11821 ttaaagcggg tgatattata gctaagattc ctcgtaacgt tggtaagtct ggcgacatca
    11881 ccggggggct tcctcgtgtt accgagcttt ttgaagcacg taacccatcg aaccctgctg
    11941 ttgtagctga aattgatgga gaagttacat ttggtaagat caaacgtggt aacagggaaa
    12001 ttaagattac ttctaaaaca ggagaagtta agaattatct tgtttccatg tcaaaacaaa
    12061 tacttgttca ggagaatgat tatgtaaaag ctggcacccc actttcagac ggagccatta
    12121 ctccttcaga tatcttagca attaaagggc ctacagcagt acaggaatat attgttaatg
    12181 aaatccagga agtttatcgt ttgcaagggg taaaaatcaa tgacaaacac tttgaagtaa
    12241 ttgttagaca aatgatgcgc aaggtagaaa tcatagatcc gggcgatacc aagttccttg
    12301 aaaaacagat tgttgacaag ctcgaattca tggcagaaaa cgacgagctc tttggcaaga
    12361 aggttgttgt tgatgcagga gattctacta cgctgaaagc tggccagatt attactgctc
    12421 gtaagcatcg tgatgaaaat tcattgttga agcgtcgaga tttgagacaa gtagaagcac
    12481 gtgatgcgtt gccagcaaca tcaagtcaga tacttcaagg tattaccagg gctgcgcttc
    12541 aaacccaaag ctggttctcg gccgcttcat tccaggaaac aacaaaggta ttgaacgaag
    12601 ctgcgatatt tggtaaagtt gacacattgg aaggtttgaa agaaaacgtt attgttggcc
    12661 atcaaatccc tgcaggtaca ggtttacgtg aatatcagaa tattattgta ggttcagccg
    12721 aagagtatgc ccgtttggtc acatctagcg attcgtcatt agatgaggtt gaagtagacg
    12781 aagaataaca ataggtttat aataaaaaaa cgccattgct tgttagcttt ggcgtttttt
    12841 tattatatta ttaacaaaat ttacatcctt catgtttatt ccctaaattt gtatcaaaaa
    12901 aggaaaagaa tatggaaaac gaaaataaaa acgagttgca gattgagtta aaagaagaag
    12961 ttgcacaagg gatatattcc aacttggcca tcataactca ttcaccttca gagtttatta
    13021 ttgactttgt aaggataatg cccgggatgc ccaaggctga tgtcaagtct cgtattatat
    13081 tgaccccgga gcatgccaaa agacttttat ttgctttgaa agacaatatt gcaaaatatg
    13141 aagcggttaa tggccagatt aaaaatgttg aacaaattcc acctattccg atgaattttg
    13201 gaggccctat agctcaggca taaaccccta aaacaataaa ctttaaaaaa tcaaattatg
    13261 gcacaaaaca cagatgtaga acaaacaaaa aaagagataa acaaagaaaa gttaaaagcc
    13321 cttcagctta ctattgataa aattgataaa gactatggca aaggaactat catgcgtatg
    13381 ggagaaaagg cagcacttga tgttcctgtt atttcatcgg ggtcaattgg gttagactat
    13441 gctttgggtg ttggaggtta tccacgaggc agggttattg agatttatgg acccgagtca
    13501 tcaggaaaga caacccttgc cattcatgct atggctgaag ctcagaaggc tggtgggatt
    13561 gcagctttta ttgatgcaga acatgctttt gaccgctttt atgctgaaaa attgggcgtt
    13621 gatatcgaaa atcttctgat ttcgcagcct gacaatggag agcaagcatt ggagattgca
    13681 gatgcattga tccgttctgg agctattgac attatagtta tagattctgt tgctgcatta
    13741 acaccaaagg cagaaattga aggagatatg ggcgattcaa agcttggatt gcaggcccgc
    13801 cttatgtcac aggctttgcg taagcttact gccaatatta acaaaacaaa tacctgctgt
    13861 atttttatta accagctccg tgaaaagatt ggggttatgt ttggtaaccc agaaacgact
    13921 actggcggta atgctttaaa gttttatgct tctgtacgaa ttgatattcg taagaaccaa
    13981 cagattaaag atggggaaga gatacttgga gtacgtacca gggtaaaaat tgtaaaaaac
    14041 aaacttgctc caccttttaa aaaggccgat ttcgacatta tgtatggtga aggtattagt
    14101 aaatctggcg agattgttga ccttggagtt gaactcgaaa ttatcaaaaa gagcggttcc
    14161 tggtttagtt atggcgacac caaacttgga caaggacgag atgccgtaaa aaatctattg
    14221 cgtgataatc ctgaattgat gctcgaactt gaacaaaaga tttttaaagc tcttcaaact
    14281 ggaggaaaag tgctttttac tgacaaagca aattaatcgt attaatttgt tgcacatgaa
    14341 ttcttatgca acatacaaag cctatagcca tgaagtttat ttttggttat tggcttttat
    14401 tgctatctca atgccatata tggagctatt ttccattatt gggttgatcc ttttgtttat
    14461 caactggata ttagaaggaa atttttctca caagctttct atattcaagc ataaaaagag
    14521 tattagtgtt ttttcggcaa tattttttat acatgtaatt tggcttttag gtacatctca
    14581 atgggatgag gcgtttcgaa atatattcat aaagctccct ttattaatat tgccagtcat
    14641 tattgggact tctgaaccca tcaaaattcc gaaagtaaga aaactacttc agctttttat
    14701 cggttctgta tccttagcag ttgtagccca ttttattatt tcaaagaaat ggttgcctgt
    14761 cgattcagat aaaatactaa aatctgggat ttttgtctct aaatctcaat taacctgcat
    14821 ggtggttgtt tctaccctta ctctttttca ttggataaaa tcaatgggcc ggaatttcaa
    14881 agcatctttg gtggtctata ttgttttagc tatactaatg ttggtttatc tggcttcaat
    14941 acattcactt gccgggttaa tagtgttggc aataacattt ttgatattgg ttatctcata
    15001 tatcaacagg ttggagaatc aaatgctgaa aataggattg attatgttga tttatgccat
    15061 tccttttttc tctattgttt tgatgataaa aacgtaccac cagttttttc attcaaaaca
    15121 acctgaatac aatgttagtc aattatttac taaagccggc aatccatatt acaatcagag
    15181 cgcaagttgg gagatagaaa atgggaacaa agtatgggtc aatgtttgcg agaaagagct
    15241 aagcaaagaa tggaataaga ttagtgagat taaatatgag gatttagatt taaaagggca
    15301 gcctttgcgg ttgacactca taaggtatat gacttcaaaa ggtttgaaaa aagatgcaga
    15361 agggatagct ttattatctg gacgcgacat aatcaatgtt caaaagggta tgaccaacta
    15421 tatctttgac acacattggt ctttattaac ccgtatttac gaagtatttt gggaagtgca
    15481 tcagtttaag cattcaaaat gttcgagtca ggaacatagc ctttctgtac gcttagaaaa
    15541 atatcatata gcattatctg ttgctgccaa acatttgttt tttggggttg ggaccggtga
    15601 tttggatttg atatacagtg atttgaataa aggccacaag atatgcctga aggcagaaga
    15661 taaaaatgat actggcaacc agtttttata tgttctttgt tcttttggta ttatcgggct
    15721 tatacttttt atttttgcat tggttttacc cggaatattg gaaatgaagt gggattcata
    15781 ctattttcta atgatattct gtgttattgt tctgtcattt tttgtttttg aagtattgga
    15841 aagctttgca gggacaatat tttgcacttt cttttattca ttttaccttt ggggtacata
    15901 tcgaaatctt gaataagatg aaccgacttg atgacgcttt ttttcaaaga gatgtacttg
    15961 atttggcccc gttgcttttg ggaaaaacca ttgtccggaa attcgatgat agctctgaat
    16021 ccagattttt aatcacagaa acagaggctt ataaaggaga agaagattta gcctgccatg
    16081 catcaaaagg tcgcacgcca aggacagaag taatgtacca accgggaggt tgcttatatg
    16141 tgtaccttat ttacgggatg tactggatgc tcaatattgt aactggcgaa aaagacaatc
    16201 ctcaggctgt gcttatcagg ggcctcgatg ctgtatatgg ccccggcagg ctgactaaaa
    16261 aacttcagat tgacaaatct tttaacggcg agcgtttgca gacttcagaa aggatatatc
    16321 tgctaaataa tgatttgaaa tgcgattata gtgttgcccc cagggttggt attgattatg
    16381 caggcgagta ctggcgggat gtcccgtggc gttttacttt gaagctttag ggcggggcaa
    16441 aattttatct atctggcaga taaaactcag gctaaagttg taatcaaaca atttttgatc
    16501 aagaatttga tatccctcaa atccgcattt gaaacttaat gcatttacta tctctttggt
    16561 atagtttacc tttattatgc ctattttacg tgtattgtag agcgtatcct ttcgctcggg
    16621 ctgagtagag atgcattggt acatggcttc acctcttggg cctatgaatt tgtggctata
    16681 ccagtaagaa aaacttgcat ccagcagtcc tgaatttact gatatttctg gtagtaaagc
    16741 atagcctgat ttgaaaggcc gggtttcaac ctgagtattt ttgttgtaaa gaaaagctct
    16801 tgaacctatt tgcaaatctt tgaatatgcc caagtctatc ttaaaactgg caaacaaccc
    16861 tgaagaaaag ttaatcaatg tttcgatggg cttttttggc ctgtttatct gaccgccaag
    16921 atgagtggcg attaaatttg caggaatttt aacgctaaat gtttctgcta ccggaatgtt
    16981 aaaattcaaa gctaatccgg aagtaagctt ttcctgcaca gaatctccgt accaggtgaa
    17041 tttgtcccat gaaagccaca aatctccttt gaggttttgt ttatttaaca tgaattgaac
    17101 gccattgtcg gcctgtccaa gataaaacct ctcagggtca agcataggtg caggttgcaa
    17161 gtggttgtca ttgaaatgca acgttcctaa agtgaagcag agggtagggg ttattctgta
    17221 atcaatgcgg taaacaggta agattttatt gaaattatcc tggcctgaat acttcaaagc
    17281 atgcaacccg ccttcaatgg tggctttatc tgtgatgtta taactgattg taggggttgc
    17341 ccaaaggcct ataagggtat agccttctac aacatcattg aaatattcgt tatccttgac
    17401 aaagttggca ttttgaaaat gaaaagccag gttagcttta ctgtttaggc ttgaatcctg
    17461 tatattttcc tgaccattaa gtaataaaga actgaagata aaagctatgt gaacgagttt
    17521 cttcatagtt ggcgtttata agtgcattag aacatttgtc agcacaaggg ataccgctga
    17581 gcgggaatac aaaaactctg ttttttgcct gtcgggtgat aaagctgaaa aattagcgtt
    17641 gaacctggtt tcaagctctt tttttatttc atcaatagcc atattgaccc atatttcgga
    17701 cattggtgtc agcccttctt tttgcaaata ttgaagacga tccatgccaa gtttaagcaa
    17761 ttgttgatat tgcttaaggt tttcatcatt ataaacagca atgctgttta tttgcgtttg
    17821 gatatcattt atggcgttat tcatagcttt tagtttagaa caaatataaa ctaatttgta
    17881 caattgatat cctttgataa aaaagaaggt gaccgtttag ccaccttctt tcataaatta
    17941 ttttacatct tcgtgatgtt tctttacata atcaataatt tcgtccacct ggcagaaggc
    18001 tatagcagta taagtatcgg ccgcgccata ataaatagca atacggccgg tttctgcatc
    18061 ataaatattt tgacagggaa aaaggacgtt aggtacaaaa cctgttgttt cataaggctt
    18121 ttctggagtc atcaaataat cgccgcatct gtattttacg atggaagggt cgttaatatc
    18181 cagaattact gcactgaagc tataaacaaa gccattgcat gtgcctgaaa ccccatggta
    18241 gaacaatagc catccttcag aagtctcgat tggaacaggg cctgcaccaa tcttagtgcc
    18301 ttgccaccag cctttaccac ctttgcccat tacccatttg tgattgcccc aatatactaa
    18361 gtctgggctt tcgctaatga aaatatcgcc aaaaggggtg tggccgctat cgctggggcg
    18421 gctcaagagt ttgtattttc cattgatttt tcttgggaac aaaacgccgt tgcggttgaa
    18481 aggcatcaat gggtttggaa ggcgcaccca tgttttcagg tcttttgtat aacccatgcc
    18541 tattgatgct ccgtgcatat cgtcacacca ggtaacatag aaagtatcgt caatcttgac
    18601 aaaacgaggg tcgtacgcat agctaacgag gtttggcttg ccattttcgt cttgccatgg
    18661 aataacattg tcgtcaatgt caaagttgat accgtctttg ctgcgtccaa aatgtaagaa
    18721 agcccttccg ttcttgtggt cggcacggaa tacaccgata aactcgccgt tatgaggcaa
    18781 aacagcactg ttataaatac gagctgttgt aggagttgga ttccagtcaa taatagggtt
    18841 tttactataa cgccagatag gcaaatgaag ccctgcgggt ttctcttccc acggaatgtt
    18901 aggcaggttc tgcccaataa ttgttttact cataaataaa ttaaatatta gttggtttta
    18961 aaatgttaca aagttacttc aatctggttt tcagcgttga gtttttcaac aggaataaag
    19021 ttgtttccaa gcttttcgcc atttaccata agctgtttta ctcctttctg ttttttgtca
    19081 ggatttttca ctgtgatatt cagtttttta cctctgaatc tgcggactac agagaattct
    19141 ccccagcctt cggggataca agggtctata accatgccat tgtaatcagg cttgatgcct
    19201 aaaatgccct gagaggctgc aaaataagcc caggtagctg ctcctgtcag ccagggcaag
    19261 cgtgatgcac catagcggtc gtggtatttg ctgtgtgttg actggctata tacgtatggc
    19321 tctatctccc tgatttcagc tttattattg catgaagcag gcaagtatgc tttgtaatat
    19381 tcaaaagcac gattgccatt gccaataagg gtttcggcca gtactaccca gccttgagtg
    19441 tggcagaata tggctccatt ttcttttgat ccggggttaa ataaagtggc tttaataaca
    19501 ttcaaatcgg cttttttata aggtgggtca catatcatgg ttccatagtc tgttgccagt
    19561 ctttctttca ctatatccat caccttaata ctccggtcag cattggcatg gccgctaagt
    19621 acagcccagg actgaggatt gagccacaat gaaccttctt catctccgtc ggtaccaaat
    19681 ttgaggccat cttcgcggta tgcacgcaga taccaccgtc catcccaggc atgcttttca
    19741 atattttcat cgagaatagc aagtttctcc aaagcccatt tcttttcggc tgcttcgccc
    19801 aataattcgc atatttctgc gtattccttc aaagcatagc gtaactggag ggccacaaac
    19861 acagtttcgc ctttgtgccc caataccaaa cagtcattcc agtcggcaac taatccacat
    19921 ggcaatccgt gagcgccgga gcgttcgagg ttaaactcaa tggccctgcg caggtgcccc
    19981 aacacggtag cttcgccttg gtctgcgaaa ggaagggttt tgcggtagaa attaatatcg
    20041 ccggtttctt taacataagc aggtatggta ttgaaaagcc acatacagtc gtccgagcgg
    20101 tattctgttt cttcggtttt aggcatttgg cccggataat ggccaaactg ttttacaaca
    20161 ggcatggcac ctccggtaga tacttgtccg gtaatcatca actcaaggcg ttgacgggct
    20221 tcttcgggta caaggtggat aacgccaagt atatcctgaa ctgtatcgcg ataacctaat
    20281 ccatcccttt cgccagcata tattaagctg gccgcacgtg accaggtgaa ggtcatcatg
    20341 ctgttataag ggttccacat gttaaacata ctatcgaata aggcatctgg agatttaaca
    20401 tgcatacctt ccaaacggct atgccagaag ttgacaaggt cgtcaaattt tttgtttaca
    20461 gcattgatgt ctgatgcttc tttgatggct ttgatgccaa ctgtatcagc ttttccaact
    20521 cccaaaacca ctacaaattc tttggtttcg ccagcttcaa gttctatgtc aatttgaata
    20581 gtgccgcagc cgttgtcccc ggctttgcaa gaacctgtgc attgtccatt ttctacaaca
    20641 acagggttat gataactcct gtatgtgccc aagaatgctt ttcggtcagt atcaaatctg
    20701 gttggtgtag cacccgcaac cccaataaac gaatagcgcg attggcggtc gttttcaaac
    20761 tcctcaggga tgtgtggcag gttgacattg gtaccagcac tgataatgtt gttcactaca
    20821 tccattttca acacatactg ggtgtattga aggttgataa tgtcttgcga gagtacccag
    20881 tggctggcaa actcaacata agtaaacaag cgaattttgc gttttcggtt gcttttgttg
    20941 ctcactttta cttgccagta ttcatattcg cttccaagag gtacaaaata tctcgtgtta
    21001 gtaagtatct cagcatactc tgattcgata atagtgtatg ccgagccatg tctgcattca
    21061 gacttaaatt tatcaagcgg tttaccaaca ggttgccacg aagttgacca aaaatcttta
    21121 ctttcacaat cgtgaacata gatgtacctt cctggttggt ccatggggat ggtatttgtt
    21181 ctgtaacgca tgaacctgcc ttgagctgct gatttgtaaa agctgtatcc gccagcgttg
    21241 ttggtaataa tggccccata agttgtagag cccaggtagt tactccacga gaggggagtg
    21301 tcaggacggg taattacata ctccttcctt ttgtcgtcaa aatatccgta attcatggtg
    21361 gtgttgtttg tatttaacat taaaccccat actatgtaag cacagggttt aacaagttat
    21421 aattttaaga aaaaattaga tagcagaaga gccgctttca atccgtcttt gattcagatc
    21481 ttcctggact tttttcatgg ttttttcatc caatttatag aaaaaaatca gtaatgtcaa
    21541 ggctaagagt cctggaatta cagggattac actcatcatt gattttaatc catttaaagt
    21601 accagcattt tgggtaacat tagcaacaaa accggtactg gccagaatga aagcagcaat
    21661 catacttcca aatgcccaac ctaatttgtt tgacatgata gatgccgaaa aaattaaacc
    21721 tgtagcgcgg cgtccggttt tccactcaga atagtcggca gtgtcggcat acattaccca
    21781 tagtaaagca gaaatagggc cacctgttat tgaaccaatg gcttggaaaa ataaaataag
    21841 aaaaatattt tcagggctga agaaaatgaa tgatcctgtg cagattaatg ctgtaacaaa
    21901 caaaataata gtagcttctt tgcggccaaa agtttttgca aaccacggaa caagtattac
    21961 ccctattaat gataaggttt gtccgacagt attaaatatt gaagttagcg cttcaaagcc
    22021 aaaagtttca tttctgttta aaacggtcaa tttctcgttt ccaaacaaac tgataatggg
    22081 gttaataata taccccctaa agaagttgag aatatcagtc agccagggca ctgattgttc
    22141 cccaacgtaa tatttgaaat agtgtgtagt aacattcaga cggatgcagt taaaaaggat
    22201 aaagagaatg gtaatagcaa aaagcaccat ccatgggata tttttcgaaa tgtctttcag
    22261 gtcgctttta atattggttt tttctttggc aataggtttg atacgttctt ttgttgaaag
    22321 gaaagtaata aagaaaaata atacagcagc aatagcatag attaacatag ttgtatgcca
    22381 tcctgattgg gcaatttcca attctgtacc acctttccca aacattttgg caaaaggcag
    22441 cgctgtagcc gaaacaatga ttccggccag ataagctcca atgaatttga aagatgaagc
    22501 gctggtgcgg tcaacagcat cattactgat aaccccaagt aaagaggtat aaggaatgtt
    22561 aactgttgag tacaaaaaca taaatgataa aaaggtaata taggcgtaaa caattttacc
    22621 tgttcctcca aaacttggta ctgtaaaagc tgcgacagca gcaacagcca aaggcacagc
    22681 accaaaaagg atataaggac ggaattttcc ccagcgggtt tcggtccggt cggctgtcat
    22741 acctatcact acgtcaaaaa atgcatcaag tacgcgtgat acaaaaaaca ttacaccagc
    22801 tgtagctgca gtaagcccaa aaacatctgt ataaaaataa agcaggtaaa ccatgatggt
    22861 ttgccaaaac aacacagagc caaggtctcc aaaaccgtag cctacctttt ccctgaattt
    22921 taatttagtg tgagttgatt ccataaaaaa tctttcatta gtggttttta ataaggccgt
    22981 aaatgtataa ttaacttata aatgaaaaaa atttttttaa ccaaaaactt tatgctttac
    23041 atcaataaac taagcattca tcaaaatcgt acctgacaaa aaataaaatc gggttagaac
    23101 ttatcaaaaa tgtctaacat ttttaaaagg cttgtttata actattttta gggagaatgt
    23161 ttaaaaaaac agaaaaatat tcattataaa acattcattt tcaataaaaa aagatacgaa
    23221 tacttgcaaa ttgttaagtt ttttttatat attcgcacaa acctaaaaga tttcaacaat
    23281 ttattaacag tataaccgtt gagctatgcc atacagacgc ctacccaata cagatgttgc
    23341 acgtatcaaa gcaatgaaaa tagcttataa caaagccaag gaattgcctc cttttaaatt
    23401 ggcattctca caaagtttat tccagcgttt acaatcgttt atgccgcttt ttgaaaagaa
    23461 tgtattggaa agcaagcaaa cttatgccaa taaagtgaac aagagtaaag attatcaaca
    23521 ggcattgcgc aaggcgaggt tatatatctc tcatttcctt caggtaatga atatggcaat
    23581 acaacgtgga gagcaacaac ctgctattcg tgaatttttt gggatagacc ctaataactc
    23641 ccgtttgcca aatcttattt ctgaagagtc cgtgcttgaa tggggcgaaa aacttattaa
    23701 aggcgaacag gaacgcatca aaagggggat gacacctata acaaacccga caatagcggt
    23761 tgtaaaagtg aggtacgagc agtttaaaga gacttatcat gcccacaaaa taatgcaaaa
    23821 gagttcatct cgttatgtga atgagttagc ccagctcaga aaaactgcag acgaaataat
    23881 tgccctaatt tgggacgatg tagaaaaaac atttgccaat ttgcctgaag agttaaggcg
    23941 tgagaagtgt aatgagtatg gtattattta tgtttttcgt aaaaacgagt taagccgtat
    24001 atctttacct tttgttaaag cagctccaag cttgtttgac agtatattgt aaactttttc
    24061 tttgtcaatt tctttcactt caccaggttg catattgtct aacttgatgt tgccaattgc
    24121 tatacgtaac aaccttaatg ttggaaaacc agcttttgct gtcattttcc ttacctgcct
    24181 gttcttccct tctatcaacg ttagctcaat ccaagaggta ggcactgttt tacgaaacct
    24241 gaccggagga acccgtggag gtatcaatgg ttcaatatct ataatcctgg cttttgcagg
    24301 ttgagtcata tagggctttc cgttgttgtt tatttcaagg ccagactcca gcatttttat
    24361 agcttctgca tcaatagcgc cttctacctg cactaaatat ttccgtgggt gttggttttt
    24421 ggggttaagc aataatttat ttatagcagt gtcattggtc aatagcaaaa gcccctcact
    24481 gtcagcatcc aggcggccta cagggtaaac atccttaggg aatgtaaaat caaggtcttt
    24541 cagcgtctgg tgttcacctt caggggtaaa ctggctcagc atgttataag gtttgtatat
    24601 gacaaaataa cgcaacatca gacgataatg gcacaaaatg tataattttg aacaaaaata
    24661 acactttgca tttatgcatg acggaacaat aatagaagca aacaaaaata ttttcttcaa
    24721 ccgcattgac gacgaaatag tcatgatgga cgaggctcag ggaaagtact attcattgaa
    24781 ccctgtggca agtcgtattt ttgaaattgt acagcagaaa tgttcgttta ggcaattgct
    24841 tgaccaatta ctgtcggaat atgaaataac tgaagaagaa tgtaaaaccc aaaccattgt
    24901 ttttttgaat gacttaaaat ctaaaaacct catttttatt gatgagtaaa tcaaagcttc
    24961 ataatttttt ccgattgtcg gctactgata tgttttattt ttttgtagcc ctttatttta
    25021 gcgtgttata ttggatttgt acaaggcttt tgccttattc tgtttggaaa aacattctgg
    25081 gaacagcaac aaaaacaggt accccatctt taaaggagca agaaaaaagc aagtttcgaa
    25141 cggtaattat agctgtaaac cgctgcagca ggcatgttcc ttttgcccgc aattgcctga
    25201 taaaagcgct tactactcgc aaaatactca ggatgaaagg tataaaaagc acgctttgcc
    25261 tggggcttaa caatgagaat aagaaattat tgactgccca tgcctgggta aaaattggcc
    25321 cggatttgac aataggagaa agtaagaacg aaaactttat tgtggtggtg atgtataaat
    25381 aaagtgatag ttcgatagta tctctgtcat cattataaaa tcgtcatatt atcgaatgtg
    25441 ttttagatat agttggaaac attgttaaga aagtcatttg caatgctttg tctaaatttg
    25501 tatatttgta tctatgaatt taattgagct aaatagagat aggttatttg aactttgtga
    25561 tagtcataag gttaaagaac tatatttatt tggttcggta ttgacggaga aattcaacga
    25621 ttcaagtgat attgatatgt tgattcaatt ctctcaagtt gaattggttt attattttga
    25681 caactatatg gatttaaagg agaagttaga agacttgttt aataggcctg ttgacttagt
    25741 tgagaatcag gcgattagaa atccagtttt tagacaaatt attgacagag ataaacatct
    25801 agtgtatgaa cgaaaaagtg cttaaaagtc tatatgatat caaacttgct attgaagaaa
    25861 tagatacttt cttttccact cgtgataaaa gatttgacga ttacactaat gatttattgc
    25921 taaaaagagc aattgagaga aatcttgaaa taattggaga agcaatgaat aggattctta
    25981 aagaagatcc tcaaatttca attgataatg caaaaagaat agttggatta aggaatcaaa
    26041 tcattcatgg ctatgatagt gtttcagatg aaaatatttg ggggattgta ataaatcatt
    26101 tgccaaaact taaagacgaa atctccaaat taatacaata aacaatgttt tcagcatgtg
    26161 gacataaaat attggagttt aatgaatttg ccaggtttgt ctcgcagcaa agttcttatt
    26221 tgatgcaagg ttcgctgtgt ttgcttcata aaactaacgt ttaggatata aataataaaa
    26281 taataaaagg ctgtgaagta cagcctttta tcgtgttagt gtttaattaa taattagttt
    26341 agaaaagtta gtgtagttag cgaaccaaaa ttttgtggtg gaaaattagc aatgttttta
    26401 ttaaatccat gaaaaaagat taattttttc aaaaaatagt tgttcagatg caattttggt
    26461 agtttttata cgttattcaa gctctttttt gtctgagaaa gtgaaatata gttttatttc
    26521 cggttctaat aaaactagca atatggatta tgtatgcttg gcatagtttt gaaaaggtaa
    26581 agggtggctg taaataccta t
//
LOCUS       DAOSPF010000048        26599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1820_length_26599_cov_7.088834, whole genome shotgun sequence.
ACCESSION   DAOSPF010000048 DAOSPF010000000
VERSION     DAOSPF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_26599_cov_7.088834"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK252_10025"
     CDS             <1..364
                     /locus_tag="PK252_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOT15084.1"
                     /translation="YYMLKILKNKWVVAFAIIIILTPLGIFLPEYFKAGDAWGEWSTE
                     QVKEQTGSVPEGMKKEAELYKAPIADYTLNEESSMAKQSLYYILSAIIGVGIIFILSF
                     GLKKLVSKNDRDPKLPAY"
     gene            333..1283
                     /locus_tag="PK252_10030"
     CDS             333..1283
                     /locus_tag="PK252_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOT15085.1"
                     /translation="MTEIPSFLLINNPVTLHEVKKNDKKFRFSFIEQTLNSSGKFIST
                     TVFQYHSALFKGILQQLNPVVKLLMLLVGTTGIALIHSVLFQLILSFLIFLLVAFSKL
                     RLRSFYKRVLTLVLIFGIIPTLPIYFNWITPGKPALELIKSGCSFNFWIYHVPNSLYI
                     SYEGIHHALRLEIKVLNSLALSLLIIHTTQFVDIVKAASRLKAPAIFLLIITLSHKLI
                     FIFTKVLEDSFLGLKSRWWRKIDENASRQIVGNRFLLVFNKTWHRYDQTYKAMIARGF
                     NGTIEHGYQKQLNRLDILFLLLFIVVLSTAVTISNVFQLY"
     gene            1286..2104
                     /locus_tag="PK252_10035"
     CDS             1286..2104
                     /locus_tag="PK252_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT15086.1"
                     /translation="MEKLFNLENVRYQYNQFQQALVNVSFDIEKGEILSIIGSNGSGK
                     STLLHILAGLVFPTSGILKFENHEISESALNKKDFNAYFRQNVGYIFQHADAQLFCPT
                     VLDEILFGPQQKLMKKEEAMDIAFSLMKMLNIEYLKDRPTYMLSGGEKKKVAIAAVLA
                     VNPQVLIMDEPTSSLDPKTRSFIIELIFSLNEAGKTIILTSHNLEMISHFQSRVAVLS
                     ENHTIERIGMPEDVLNDTEFLIKNNLISEHMHKHGTHIHKHLPGSLFFHKHDRL"
     gene            complement(2101..2619)
                     /locus_tag="PK252_10040"
     CDS             complement(2101..2619)
                     /locus_tag="PK252_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT15087.1"
                     /translation="MTFLELAKKRFSSRKYLDKGIERSKLELLLEAARVAPSAVNKQP
                     WLFYVFSSPEGLEKIRPCYHRDWFKTAPVVIVAVADHKAGWVRSSDGKDHCDIDLAIA
                     IDHITLQATDLGLSTCWVCNFDVDACKKALQLSDSFEPVALIPVGYPAEEKESIRFET
                     ERKSLNTIVRWL"
     gene            complement(2616..3452)
                     /gene="kdsA"
                     /locus_tag="PK252_10045"
     CDS             complement(2616..3452)
                     /gene="kdsA"
                     /locus_tag="PK252_10045"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOT15088.1"
                     /translation="MFEKVFSKLENIKHLDSGNFFLIAGPCVVENELIAFETAETIAA
                     ITDKLKIPYIYKSSYKKANRQKLDSFTGIGDEKALRILRTVGEAIEVPVITDIHTEDD
                     AFLTSQYVDALQIPAFLCRQTDLLLAAARTGKVVNIKKGQFLAPESMKFAAQKVVESG
                     NEAVMLTERGTMFGYHDMIVDYRSIVDMKKFGHPVVLDITHSLQQPNLSSGVSGGRPD
                     MIETIALAGIAAGADGIFIETHPNPAEAKSDGANMLKLDLLEDLLIKMVAIRQTINQF
                     KK"
     gene            complement(3528..3893)
                     /locus_tag="PK252_10050"
     CDS             complement(3528..3893)
                     /locus_tag="PK252_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15089.1"
                     /translation="MKVLIVEDDSMTRKALEHRMKIDGYDVESAPDGIKGSEILKAGK
                     IDILLTDLHMPNFSGLDLIKYVRNELRSNIPIIMVTRVGLEETVLNAFELGADDYITK
                     PFSPDELSLRVKKAVMKNK"
     gene            complement(3905..4246)
                     /locus_tag="PK252_10055"
     CDS             complement(3905..4246)
                     /locus_tag="PK252_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15090.1"
                     /translation="MMEYNLDYLKEISGGDPEFIKDMLGEFISKTPETIAEVEETIVK
                     KSHEELYKVIHRFIPTFDFIGASHIISNLRNIETLSKTKGSFEEIVVIFDKVKEQSTN
                     LIATLKKEFAL"
     gene            complement(4475..4624)
                     /locus_tag="PK252_10060"
     CDS             complement(4475..4624)
                     /locus_tag="PK252_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15091.1"
                     /translation="MKKLKKQVRKNEKVLLKNKEDLKYLIFAGIPAGIIMILLSKCGW
                     LGRAF"
     gene            4785..6083
                     /locus_tag="PK252_10065"
     CDS             4785..6083
                     /locus_tag="PK252_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT15092.1"
                     /translation="MQSNQPLAERMRPKNLEEYIGQEHLVGKNGALRRSIDSGALPSL
                     ILWGPPGVGKTTLANLLAQKLNRTFFTLSAVHSGVKDVRETIEKAKSHAFMGTPSPIL
                     FIDEIHRFSKSQQDSLLMAVEKGIVTLIGATTENPSFEVISPLLSRSQVYILKPLEEQ
                     HLKTILDRALEKDFLLKQANVELKETEALMRISGGDARRLLNALEIVISTLKSEIQDS
                     SIPIVITNNVVLSIIQKNIAIYDKDGEQHYDVISAFIKSVRGSDPNAAVYYLARMIES
                     GEDIEFIARRLIILAAEDIGLANPNALLLANNCFQAIHAIGMPEARIILSETTLYLAC
                     SPKSNAAYLAIDEALGYVRETGNLPVPMQLRNAPTNLMKKIGYGKDYKYSHDGPGHFV
                     EQEFMPKEIVGKVFYNPAENQRENEMRAYLKKCWNKKYNY"
     gene            6093..6746
                     /locus_tag="PK252_10070"
     CDS             6093..6746
                     /locus_tag="PK252_10070"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOT15093.1"
                     /translation="MTSIDKAVLLSTAYMPPIQYFTKCVLYKDIYIEGCENYTKQSFR
                     NRCDIATANGVLTLTIPVVHAEGQNKIPIKEVLIDNNKRWKAIHKKAIESAYRSSPFF
                     IYYADDLFSLIDAPSDKLFDFNTSILKFLLEQLEIKPQINITTDYKTIDRAFDDWSEG
                     IHPKTSRQKEDTTYISKPYYQVFQQKYGFIANLSIIDALFNLGPGTNEYLRSCVKTK"
     gene            complement(6815..7924)
                     /locus_tag="PK252_10075"
     CDS             complement(6815..7924)
                     /locus_tag="PK252_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOT15094.1"
                     /translation="MILEQLTLANFKNYEQAEFSFSAKINCFVGNNGVGKTNILDAIH
                     YLSMCKSYFNSADSQNIRHNEQFFSIRGIFTGSSDNKEEIFCGFKRQRGKIMRRNRKE
                     YNRLADHIGFIPVVMVTPSDSSLITEGSEERRRLIDAIISQYDKHYLDDLILYNRVLL
                     QRNKALKSFAFSGSFDVQMLEVYNEQLVDVGNKIFAKRKEFFDSFLPVFQKYYNFIAG
                     TDEMARLEYESQLNNADFTTLLSQNINKDRALEYTSAGIHKDDMVLMLNEYPVKRIAS
                     QGQQKTYLVALKLAEFDFIEKHSAKKPILLLDDIFDKFDAERVKQIINLASDKHFGQI
                     FITDTETERIQKILAEISFEHKIFTVDKTGSIKIT"
     gene            8170..10356
                     /locus_tag="PK252_10080"
     CDS             8170..10356
                     /locus_tag="PK252_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT15095.1"
                     /translation="MWGKVCTCLLISVVELSCLLASEKPELSDFVSKSTFKENRADNI
                     LPSSNELYYYSLTNNMVIRYKFADGLSVDTPVNNLSTAFDKNIDIASFSFDNSEHQFL
                     LAYNIKPLYRYSFYASYVIFNSLTNECIKVSEGKKIRDAQISPDGNKVAYCYKHNLYV
                     FDVVQNKTTQITNDGDGDSIINAEPDWIYEEEFSLLQAFKWSPDSKSIVYYKLNEKKV
                     MTYPLTYYTKTNCFQADDWVYPYVVTLKYPKVGKPIAQTSLYVYDLQTKTNTMIVNVG
                     ENYITDFKWTIKSDNVFYTRVNRAQNKYNIEEYNFVSKEIKVLYNEQTNTNFEFPDVP
                     SVFFINDKNDFVFLSEKTGYKHLYLATENGSKIKQLTTGNWEVLAIENIDILNKRIFL
                     TTNQASPLQKHLYCYDLKKLKLSQLTKGNGVHKAVFSADFNNIIHTYSSLIEPLKVSI
                     MNVKSGKEKVLIDNKKMADKAIEMNVPQKDTFSFVLPSDERLYGWMIKAKKETNRPAP
                     TLFYVYGGPGIQNVLDKYEVKWYHYLAMQGYNVVSVDNRGTSNRGKSFRDATYGKLGE
                     IACTDIVATANYLGQKAWIDTSCLAIMGWSFGGFLAATCLQQSNGLFKAAISVAPPTD
                     WRLYDAAYTERYMGLLEDNEEKYNKTSLIVNADKTNGKLLLIHGTADDNVHVQNTLMF
                     ADALVKADKKFEMKLFTDDNHQINSQNAGMQLYNQVIDFLNRVFDK"
     gene            10405..11133
                     /locus_tag="PK252_10085"
     CDS             10405..11133
                     /locus_tag="PK252_10085"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT15096.1"
                     /translation="MNLIIDIGNSRIKLGVFKNNELLESFVFADMFVCDAELVLRKYR
                     DIKNCIIGATGIIPTDLLEYLSNTFPNLIVLSGNTNLPFKNLYQTKETQGPDRIAAVA
                     GAISMFPSSDVLVIDAGTAITYDFINKQGEYQGGAIAPGMTMRFKALNSFTQKLPLIA
                     AGEKAELHGKTTEESIKSGVQNGIIFEIQGFIEKHRLDNPELKIVLSGGDANFLAEQI
                     NCTIFVEQNIVLIGLNSILNYNAQ"
     gene            11123..11689
                     /gene="lptC"
                     /locus_tag="PK252_10090"
     CDS             11123..11689
                     /gene="lptC"
                     /locus_tag="PK252_10090"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOT15097.1"
                     /translation="MRNKVYIVLFISIVFITSSCENNIEVIRNMGKEKDLPQVSADNI
                     EILYSDSARVKIQIISPSIVRYNLPNKMYTEFSKGLTVNQFDTAHNIEATITAQHALY
                     RDQEKLWKAWGNVLARNHKTGEELSTEELFWDQEKGIIYSTKFSRISNADGVFYDDGG
                     FEAKEDMSQWHMIGIKGSVNIKDNGNEN"
     gene            11679..11858
                     /locus_tag="PK252_10095"
     CDS             11679..11858
                     /locus_tag="PK252_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15098.1"
                     /translation="MRTKIREYVWLSLAIFCIAAAIHQTFKLGFSESYVFFIFAVMAF
                     LIYYIRRTLRLAKKD"
     gene            complement(11931..12266)
                     /locus_tag="PK252_10100"
     CDS             complement(11931..12266)
                     /locus_tag="PK252_10100"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT15099.1"
                     /translation="MMFNYFEEVENPVTVCDANGIILYMNAASRQMLNKYGGQNLIGK
                     SLYDCHNENSNRIIRELLEHKKSNTYMVEKEGKRKLVHQTPWFENGRLAGLIEIITFL
                     PEDLKEVKR"
     gene            complement(12333..14732)
                     /locus_tag="PK252_10105"
     CDS             complement(12333..14732)
                     /locus_tag="PK252_10105"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15100.1"
                     /translation="MQYGFFDDKRKEYVITRPDTPRSWSNYLGSTEYGAIITNNAGGY
                     SFYKSGGIGRFMRIRFNSIPMDQPGRYLYIRDKESADYWSASWQPVGKPLDKYKSECR
                     HGSAYTIISSEYDNIATETTYFVPLGQIFEVWKIKVTNKDKRARKLSMFSFVEFANCW
                     NAMQDLLNLQYTQYIAKMSVVDGIIDHGTNVHIPAMPDNFAENDQGRHTFLAFVGGKV
                     VGHETDLESFLGRYRTYANPLAVETGKCSNFMAAGDNVCGTTQIDIELAPGQSQDLIV
                     LLGVGEAANEGKKVVAEFGNVARLEQELDKLIRYWHGRIDAVAVETPDAEFNSMLNMW
                     SPYNCQMTFNWSRAASLVYTAMERDGLGYRDSVQDILGVLPNIPDEAGKRLELMITGQ
                     VSTGGAMPVVNKIAHKPGKEKLPDENQYRSDDCLWLFNAVPAYVKETGDINFYNKVLP
                     YADKGEDTVLGHLRRAIQFNLDRSGKHGLPCGLLADWNDCLKFGHDGETAFVAMQLRF
                     ALKEYIQICKMLGKSEEVEWAKPKLAQIDENIQKYVWDGKWFLRGYRVDGFKFGSNES
                     KEGKIYLNPQAWAVISEAASAEQAKTAMQSVNEMLATEYGIALCNPPYTYGADYNIVR
                     SALFNPSMKENGAIFTHTQGWAVMAETLLGNGNRAYQYWRAYMPAAYNTRAEIRQIEP
                     YVYNQSTHGQYSPRFGASRLPWLSGSATWSFFVATQYILGVRPDYDGIIIDPCIPADW
                     KEFKVARRFRGKQLNIIVKNPCGVQKGVSKININNKTIDGCKIANSDLASNNEIVVIM
                     G"
     gene            complement(15059..15301)
                     /locus_tag="PK252_10110"
     CDS             complement(15059..15301)
                     /locus_tag="PK252_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15101.1"
                     /translation="MYLSIKSKSIDKSILINKTINNIKKLPVSKVKDVNDFVDFLLNR
                     IEDQVITEGIQEIASTSKSFGILNNEPDLFTGIDFK"
     gene            complement(15350..16789)
                     /gene="rmuC"
                     /locus_tag="PK252_10115"
     CDS             complement(15350..16789)
                     /gene="rmuC"
                     /locus_tag="PK252_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT15102.1"
                     /translation="MTTILLSVCIVLFVVLILLQLLNKTSGKDSKIELEKLQELQFSV
                     NKLEEKLPSEFKTNRDENAALAKENREELNNTLKEFRAELSQTFKTIIEQNNQLQKDL
                     NLVLDAKFKDIFNQAKLENQQLRETLDKVFKGFQDTFDKNVQSFNDLQKEKFGKLEVQ
                     QQDLVKNTEQKLETIRVTVEEKLEKTLSERLGQSFETVGKQLIEVQKGLGEMQTLAQD
                     VGGLKKVLSNVKMRGGIGEVQLSMLLEQILAPEQYEANVKTKQGSADVVEFAVKLPGK
                     DDNHRNVWLPVDAKFPKDVYEQLQTAYDNGDPSQIETAQKNLEQTIKKMAKDISDKYL
                     DPPSTTDFGIMFLPFEGIYAEVVRKASLLEELQKTYKIVVTGPATLAAILNSLQMGFR
                     TLAIQKRSSEVWQVLAAVKKEFDSFGGMLQKAQNNIQTGLNQLDTVMSTRTRAIQRKL
                     KGVELMNGGNEALPLEGINEDDADADEQE"
     gene            17000..18682
                     /locus_tag="PK252_10120"
     CDS             17000..18682
                     /locus_tag="PK252_10120"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT15103.1"
                     /translation="MITTRKIGIFATLLLTAVTVKAGDSLQSILQNELQRELSVLSKQ
                     SLPAYYIQYRVDDIKNYSIAASFGSLTDEDSDNSSLLNVEVRIGDYTKDNTHITKLSD
                     ERPSIPVTLALPIEKNKEAIEIALWQATHYAYRKAADEFNNIQSGMLNDAEEGTADFS
                     KETPNVYYEAPLDFNKEKLSYMRERMKNISGFFTSTPEIQSADARFSYVYQRKYFANT
                     EGTDIVQNNTYCQIQVVVVSKAEDGNYVPLVKTYSAFNPDSLPSEATIMQDMQQMLNL
                     LKQLRNAPVADAYSGPAILSPDAAAVFFHEIFGHRIEGQRMKNNFDSQIFKNKVGSRI
                     INKHLTVVSDPTMKNLGKLDMFGYYKYDDQGIKASRVELVKDGVLKDFLMSRTPIKGF
                     EHSNGHGRSQTGLSPVSRQSNLIITSNQVHSKEKLRKMLIAECKKQNKTFGYYFDQVI
                     GGFTLTNRFTPNVFNVSPVVVYRVYVDGRPDELVRGINFIGTPLAIFEGILAAGDQYG
                     VFTGYCGAESGSIPVTASAPSLLIKKIETQKKPEGLNTKPILPRPDFIKENK"
     gene            18687..20453
                     /locus_tag="PK252_10125"
     CDS             18687..20453
                     /locus_tag="PK252_10125"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOT15104.1"
                     /translation="MSKYIKHISTPILVTLLTTTISAQDSISNLIFRAMKDEIDRSIS
                     QLEYKDYAKPFYISYTIEDTRTLYVNASLGSIVNSNEKKYRSWGNRVLCGTYELNDEN
                     FMDATRNPAPSDGRLQLPIDDDYWGIRRALWIITNNTYKSAAENYRSKLEALKDKNLT
                     KDTLEIPDFAKSPTVNLIIEPKNKEWNKQLLEDKVKTISSTFLKYPDIVFSDVSIYQI
                     NANLYFYSSENSKIKQPVQFTILTVTAVCEAGNGEKFSNQLQYVVNLPSQLPSNDTII
                     SHCNALAIFLGQLQDVPVSKENYSGPVMYTGQATPEILTQCMFYGNDNLFAYREPLYN
                     NSQMSMYYGQNMNSFETKLGKLVASKNISITEFTKKDNYHGIPLIGNYQCDAEGVIPK
                     DTMVLIDKGILKTLYNGRTPTRNIPLSNGHRRYVLVNGGISNNLGPGVVEISSSEKKS
                     VTELKTMLINEAKNEGLDYAILIKPIVETNGYTPLAAFKVDVNNGSEKPIRGLSMKPL
                     NINYLKKIIGFSDKNIVYNNISEGGMSPIDGSNSGKQVIPSGNPTSIICPSAIVVKEI
                     DFEYISKALVNDKIIVPSPLKK"
     gene            20677..22110
                     /locus_tag="PK252_10130"
     CDS             20677..22110
                     /locus_tag="PK252_10130"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT15105.1"
                     /translation="MKTFKYSGIFISALAGAIMAVLISNFFFPKKQIVYNYPGYKAQN
                     VSYDPGAAQPIDFTIAAEQTVHAVVNVRTKAKNENQFVNPLYYFFYGQDEPVQQPPKV
                     GIGSGVIIDPNGYIVTNNHVIQGANEIVVTLNDNREFTAKLIGADPSTDLALLKVEAN
                     NLPVIPYGNSEELRLGQWVLAIGNPYNLSSTVTAGIVSAKARNLNIINDNYRLESFIQ
                     TDAALNPGNSGGALVNTKGELVGVNTAILSVTGGYSGNSFAIPVTIVKKVVDDLREFG
                     AVQRAFLGITPADVNAEIAKQNNLSITTGVFVADVKETGAAKEAGIKANDVITKINNV
                     PVVNTSELQEQLSKYRPGQKISVTILRNDNLKDISVTLRNLQGETKLLKPNDITTTIM
                     GATLSDITEEDKKNLGIKYGVKIVSLSPGKFSKAGIEEGFIITRLNNKAVTSVAEMKK
                     IINNTRGGLYIEGIYSNGVIAYYAFGL"
     gene            22825..26538
                     /gene="purL"
                     /locus_tag="PK252_10135"
                     /gene_synonym="purI"
     CDS             22825..26538
                     /gene="purL"
                     /locus_tag="PK252_10135"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOT15106.1"
                     /translation="MLKFFKGPENKYFTVHFATDPDAKDLQKLSWLFGATLVDSEKVD
                     GFFIGPRKEMITPWSTNAVEITQNMGIEGIKRIEEFYQVESADTKYDHMLKAMYNGLN
                     QDTFTVLREPEPVKYITDIRTYNKQEGLALNDDEIEYLESLRKKLGRDLTDSEIFGFS
                     QVNSEHCRHKIFNGTFIIDNKEKEESLFQLIKKTTKANPNQVVSAYKDNCAFIQGPVV
                     DQFAPATQDKPDFFETKPFESVISLKAETHNFPTTVEPFNGAATGSGGEIRDRIGGGK
                     GSFPIAGTAVYMTSYPRSAQGRIWENNLSERKWLYQTPEDILIKASNGASDFGNKYGQ
                     PLICGSVFTFEHFENHRKFGFDKVIMLAGGVGYAKKKDSLKDTPEPGDKVVLLGGDNY
                     RIGMGGGAVSSVATGEYNNAIELNAVQRSNPEMQKRVYNAIRALSESDHNPIVSIHDH
                     GAGGHLNCLSELVEATGGKIDISKLPVGDPTLSNKEIVGNESQERMGLVLKEKDIALL
                     KRIADRERAPMYVIGEATGDNRFTFTDPKTGKTPIDLELDDMFGNPPKTIITDNSQVI
                     SYADVTYDTSKLEEYLVQLLQLEAVACKDWLTNKVDRSVTGRVAKQQTAGAIQLPVNN
                     VAVVAMDYKGKKGIATSLGHAPAAALINASAGSVLSIAESLTNIIWAPLEHGLKGISL
                     SANWMWPCKQPGEDARLYEAVTAASNFAIELGINIPTGKDSLSMTQKYPDGDIVLSPG
                     TVIISAIAQVSDIRKVVEPVINPSVDSSILYIDLSNVSFELGGSSFAQIMNKLGSYTP
                     TIADTAYFIKAFETIQSFIKNEKIVAGHDISSGGMITSLLEMCFTSVNAGLNIDLSVI
                     NEKDIIKILFSENPGILVQVTKENLAFVQAELNKNKILFHEIGKPANNGKVKIKKDDT
                     EFEFNVAELRDIWFKTSYLLDRRQSGEFKAIERYNNYKAQPLKFNFPPSFTGKFADFG
                     INPDRKTKSGIKAAIIREKGVNGDREMAWMMHLAGFDVKDVHMTDLISGRETLEDLNM
                     IVFVGGFSNSDVLGSAKGWAGAFLYNPKAKESLDKFYARPDTLSLGVCNGCQVVIELG
                     LITPEHEQKPKMLHNDSHKFESSFLTVDIHPSNSVMLSSLAGSRLGIWVAHGEGKFSL
                     PYEESKYNIPMKYSYSAYPGNPNGSNFDTAGLCSEDGRHLVMMPHLERATYPWNWANY
                     PADRKNDEVTPWIEAFVNARKWIEKKATTLKSK"
BASE COUNT         8606 a         5101 c         4880 g         8012 t
ORIGIN      
        1 atattatatg ttgaaaatat tgaaaaataa gtgggttgtt gcatttgcaa tcatcataat
       61 ccttaccccg ctgggaatat ttttgcccga atattttaaa gctggcgatg catggggcga
      121 atggtcaaca gaacaggtaa aagagcagac aggatctgtc cccgaaggga tgaaaaaaga
      181 ggcagagctt tataaggccc caatagctga ttatacattg aatgaagaaa gctcaatggc
      241 aaagcaatct ttatattata ttttatcggc aattattggt gtaggaataa tatttatcct
      301 tagttttggc ctcaagaaac tggtttcaaa aaatgacaga gatcccaagc ttcctgctta
      361 ttaacaatcc ggtcactttg catgaggtaa agaaaaatga caaaaaattt cgcttttcat
      421 ttattgaaca gacattaaat agttcaggaa aatttatatc tactacagta ttccagtacc
      481 actcggcctt gtttaagggt attttgcaac agctaaaccc cgtggttaaa ctgttgatgc
      541 ttttagttgg gaccaccggg atagctttga ttcatagtgt tttattccag cttattttat
      601 ctttcctgat atttttattg gttgcattct caaaactcag attacgctca ttttataaaa
      661 gagtacttac attggtactc atttttggca taattccgac attacccatc tacttcaact
      721 ggattactcc tggaaaacct gcattagaac tcatcaagtc aggatgtagc ttcaactttt
      781 ggatatatca tgtaccaaac agcctatata taagctacga agggatacat catgctttaa
      841 ggctcgaaat aaaggtacta aactcgctgg cattatcatt actgataatc catactaccc
      901 aatttgttga cattgtaaaa gccgcatctc gactaaaagc gccagctata tttttgctaa
      961 ttattacgct gagccacaag ttgatattca tttttaccaa agttcttgaa gattcctttt
     1021 taggcctaaa atcacgctgg tggaggaaaa ttgacgaaaa tgcatcaagg caaattgtag
     1081 gcaacaggtt tttactggtt tttaataaaa catggcaccg atatgaccag acctataagg
     1141 ctatgattgc aagaggcttt aacgggacca tagaacatgg atatcagaaa cagctcaata
     1201 ggctggacat attgttttta ttattgttca ttgttgtttt atcaactgct gtcacaatct
     1261 caaatgtttt tcaattgtat taattatgga aaagcttttt aatcttgaaa atgtcaggta
     1321 tcagtataat caatttcagc aagcattggt taatgtaagt tttgatattg agaaaggaga
     1381 gatattatct attattggct caaatggtag tggaaaatca acgctattac atatattggc
     1441 cggattggtt tttccaacct caggaatatt aaagtttgaa aaccatgaaa tatctgaatc
     1501 agctttaaat aaaaaagatt tcaatgccta ttttaggcaa aacgttggat atatctttca
     1561 gcacgctgat gcacaacttt tctgcccaac agtattagat gaaatattgt ttggccctca
     1621 gcaaaagctt atgaagaaag aagaagctat ggatattgct ttttcattga tgaaaatgct
     1681 caacattgaa tacttgaaag acaggccaac ctatatgctt tctggcggag agaaaaagaa
     1741 agttgccata gctgctgtgc ttgctgtaaa tccacaagta cttataatgg atgagccgac
     1801 cagcagcctc gacccaaaaa cacgttcatt tattatcgag ctgatttttt cattaaatga
     1861 ggccgggaaa actatcatac taacatcgca taaccttgaa atgataagcc attttcaatc
     1921 acgtgttgct gtattgtccg aaaatcacac cattgagaga ataggtatgc ctgaagacgt
     1981 gctaaatgat acagaatttc tgataaaaaa taatttaatc agcgaacaca tgcacaaaca
     2041 tggcacacat atacacaaac acctgcctgg tagcctgttt tttcacaaac atgatagact
     2101 ttaaagccac ctgacaattg tattgagcga tttacgttct gtttcaaaac ggatactctc
     2161 tttttcttca gccggataac ctacaggaat taatgccacc ggctcaaaag aatctgataa
     2221 ttgcaatgct tttttgcaag catcaacatc aaagttacag acccagcaag tggataatcc
     2281 aaggtcagta gcttgtaagg taatatggtc tatagcaatg gcgaggtcta tatcacaatg
     2341 atcttttcca tcagaagaac gcacccagcc tgctttatgg tctgcaacag caactataac
     2401 aacaggggct gttttgaacc aatcacgatg ataacatgga cgaatttttt ctaaaccttc
     2461 aggagaagaa aaaacataaa acaaccacgg ctgtttgttt acagcagaag gggcaaccct
     2521 tgcagcttcc agcaacaatt caagtttact gcgttcaatg cctttatcaa ggtattttct
     2581 ggacgaaaac ctttttttcg ctaactcaag gaatgtcatt ttttaaattg atttattgtc
     2641 tgccgaatag ctaccatttt aatcaataaa tcttccaaaa ggtctaattt aagcatatta
     2701 gcgccatctg attttgcttc ggcaggatta ggatgagttt cgataaatat accatcagct
     2761 ccagcagcaa taccagccaa tgcaatagtt tcaatcatat ctggccggcc gcctgaaacg
     2821 ccggaagata agttgggttg ttgtaaagag tgcgtaatat caagaacaac agggtgtcca
     2881 aatttcttca tatcaacaat actacgataa tccacaatca tatcgtgata accaaacata
     2941 gtgccacgtt cggtaagcat tacggcttca ttaccagatt ctacaacctt ttgtgctgca
     3001 aatttcatcg attcgggagc taaaaactgc cctttcttaa tattcacaac ctttcccgtt
     3061 ctggctgcag ccaaaagcaa atcggtctga cggcaaagaa aagcaggtat ttgcaatgca
     3121 tcaacatatt gcgatgtaag aaaagcatca tcttcagtat ggatgtcagt tataactggt
     3181 acttctattg cttctcccac agtacggagg atacgaagtg ccttttcatc accaatgcct
     3241 gtaaatgaat ctaatttttg acggttagcc tttttatatg aagacttgta aatataagga
     3301 attttaagct tatctgtaat agcagcaata gtttcagctg tttcaaaagc aataagttca
     3361 ttttctacta cacaaggccc ggctatcaaa aagaaattgc ctgagtccaa atgtttaatg
     3421 ttttcaagtt tagagaatac tttttcgaac atcggttaag ttttagttag gcaaatttat
     3481 ttaaaattgc cagaacgaaa acaaaatggt ataacaatat gaatgattta cttatttttc
     3541 attacagctt ttttcaccct gagagaaagc tcatcggggc tgaaaggttt ggtaatgtaa
     3601 tcgtctgcgc caagttcaaa agcgttcaaa acggtttctt ccaaccctac acgggtaacc
     3661 ataatgattg gaatattact gcgcagctcg ttacggacat actttatcaa atcaagcccg
     3721 ctaaaattgg gcatatgaag atctgtcaac aagatatcta tctttccggc tttcaatatt
     3781 tcagaacctt ttatcccgtc gggggcagat tcaacatcgt aaccgtcaat tttcatccga
     3841 tgttcaagcg ctttacgcgt catactgtcg tcttcaacta tcagtacttt catattaatt
     3901 aattttataa agcaaattct tttttcaatg tagcaatcag gttggtactt tgctctttga
     3961 ccttgtcaaa tataaccact atttcttcaa aagagccttt ggttttagaa agagtttcga
     4021 tatttctcag gttcgaaatg atatgagaag caccaataaa atcaaaagta ggaataaacc
     4081 tgtggattac tttgtaaagc tcttcatgac tttttttaac aattgtctct tcaacttcag
     4141 caatagtttc aggagttttg gaaataaatt caccaagcat atcctttata aattcagggt
     4201 cgccgccaga tatttccttt aaatagtcga gattatactc catcattcac ctaaatacat
     4261 tttgtacata caaatgtatg tttttataag aaatatttgc agatgttaga agtatattat
     4321 ttgtctgaaa caaaataggt atattctgtt agttagtata aaaacatata aaacaaaaag
     4381 aactgccaga aaaacgatat tcttagcagt tcttaaaact tgaattaact taaaatttat
     4441 aaagctcttt attattaaaa tgagggttgt ccatttaaaa tgcacggcct aaccaaccac
     4501 actttgacag gagtatcatt attatccctg ccggtatgcc tgcaaaaatc aagtacttca
     4561 aatcttcctt gtttttaagc aggacttttt cattttttct aacctgcttt ttcaactttt
     4621 tcacttttta atattgtaaa tttacactca aaatagttca tttttagaat tttagccatt
     4681 aagttatcgt taagtttcta ttaaaacagt ttaatcatca tacttgtttg ggtaaaacaa
     4741 atgaaagcct ttactttgta agtaaaattt caacgtagaa tttaatgcaa agcaatcagc
     4801 cattggcaga gcggatgcgg ccaaaaaatt tagaagaata cattgggcag gaacaccttg
     4861 ttgggaaaaa cggtgctttg aggcgcagta tagactctgg agcattgcca tcgcttatcc
     4921 tgtggggacc tccgggagta ggaaaaacga cacttgccaa tttgcttgcg caaaagctga
     4981 atagaacttt ttttaccctt agtgctgttc attcgggagt gaaagatgtt cgtgaaacca
     5041 ttgaaaaagc taagagccat gctttcatgg gaacgccatc ccccatttta ttcatagatg
     5101 aaatacatcg ctttagcaaa tcgcagcagg acagcctgct tatggcagtc gaaaagggaa
     5161 tagtaacatt aataggtgct actacagaaa acccttcttt tgaagtgatt tcgccattgt
     5221 tatcgcgtag tcaggtatat attttaaagc ctttagaaga acaacattta aaaactattt
     5281 tagacagggc tttagaaaaa gactttctgc tcaaacaagc caatgtcgag ctaaaagaga
     5341 ccgaagccct gatgcgcatc tcggggggcg atgctcgtcg tttgttaaat gctctggaaa
     5401 ttgttataag tacattaaaa tctgagattc aggattcttc aattccgata gtcatcacta
     5461 ataatgtggt tttatctatc atacaaaaga atattgccat atatgataag gatggcgaac
     5521 aacattacga tgtaatatct gcttttatta agtcagtaag aggcagtgac cctaatgctg
     5581 ctgtatatta ccttgcccgg atgatagaga gtggcgaaga cattgaattt attgcccgta
     5641 ggttgattat ccttgcagca gaagatatag ggcttgccaa tcccaatgct ttattgttgg
     5701 caaacaactg ttttcaagct attcatgcaa taggaatgcc tgaagcccgc attattctgt
     5761 cagagacaac attgtacctt gcctgttcgc ctaaaagtaa tgcagcatat cttgccattg
     5821 acgaagcttt aggttatgtt agagaaacag ggaatttgcc agtgccgatg cagctgcgta
     5881 acgcccctac taatctgatg aaaaagattg gatacggaaa agactataaa tactcgcatg
     5941 atggccccgg acattttgta gaacaagaat ttatgccaaa agagattgtt ggaaaagtgt
     6001 tttacaatcc ggccgagaac cagcgcgaga atgagatgcg tgcatacctg aagaagtgtt
     6061 ggaacaagaa gtacaattat taagaacatt gtatgaccag tattgacaaa gctgtattac
     6121 tatcaacagc atatatgccc ccaattcagt attttaccaa gtgtgtttta tataaagata
     6181 tttatataga aggatgtgag aactatacca aacaaagttt ccgcaacagg tgtgatatag
     6241 caacagcaaa tggagtattg actcttacta ttcctgttgt acatgctgaa ggtcaaaaca
     6301 aaatcccgat caaagaagta cttatagata ataataaacg ttggaaagct atccataaaa
     6361 aggctataga atcagcatat cgttcttcac ctttctttat ctactatgca gacgatttgt
     6421 ttagcttgat tgatgctcca tctgataaac tctttgattt taatacttct attttgaaat
     6481 ttttgttgga gcaactggaa ataaagcctc agataaatat cactaccgat tacaaaacta
     6541 ttgacagggc ttttgacgat tggtcagagg gaatccaccc taaaacctca agacagaaag
     6601 aagatacaac gtatatctca aaaccttatt atcaggtctt tcaacaaaag tatggattta
     6661 tagcaaatct gagtattata gatgctttat tcaatctggg gccagggaca aacgaatatt
     6721 tgagaagttg tgttaaaaca aaataaaacc accccaacct cccgcctgat acaggacaag
     6781 ctctccttgt aaaggaggag gaaaaaaata gtatttaagt aatcttaata gagccagttt
     6841 tatcaactgt aaaaatcttg tgttcaaagc ttatttcagc caatatcttt tgaatccttt
     6901 cagtttcggt atctgtaata aaaatttgtc caaaatgttt gtccgaagcg aggttgatga
     6961 tttgtttcac acgctcggca tcaaacttgt caaaaatgtc atcgagcaaa aggataggtt
     7021 tcttggcact atgcttttca atgaagtcaa attctgccaa ttttagggca acaagatagg
     7081 tcttttgttg tccctgcgaa gcaatacgct ttacaggata ttcgttcagc atcaatacca
     7141 tatcatcctt gtgaatacca gcgctcgtat attctaaagc cctgtcttta ttgatatttt
     7201 gacttaacaa tgtagtaaag tctgcattat taagctgaga ctcgtattcg agcctggcca
     7261 tctcgtcagt cccggctatg aagttgtaat acttctgaaa aacaggtaaa aatgaatcaa
     7321 aaaattcttt tcgtttggca aaaatcttat ttccaacatc tacaagctgc tcattatata
     7381 cttcgagcat ttgaacgtca aaagaacccg aaaaagcaaa tgatttgagg gctttatttc
     7441 gctgcaacag cacacggttg tataaaatca ggtcgtcgag ataatgttta tcgtattgcg
     7501 aaataatagc atcgataagc ctgcgacgct cctcactacc ttcagtaatc aagctgctat
     7561 ccgaaggtgt taccattaca acaggaataa aaccaatatg gtctgccagt cggttgtatt
     7621 cttttcggtt acggcgcata attttgcccc tttgcctttt gaaaccacag aatatttctt
     7681 ccttattgtc tgagctacct gtaaatatac cacgaatact gaaaaactgt tcgttgtgcc
     7741 tgatgttttg gctatcagca ctattgaaat acgatttaca cattgacaaa tagtggatag
     7801 catcaaggat gttggtcttg ccaacaccat tgttaccaac gaaacagttg attttggccg
     7861 aaaaggaaaa ttcagcctgc tcgtagtttt taaaatttgc cagtgtcagt tgttcaagta
     7921 tcaaaagcag tttttaattt ttacaaaagt aacattattg gctttttgat gttaacctat
     7981 tttttaacaa gcaaattggg tttgttaatt atctttgata atgttattgc aattggcaag
     8041 aactggttga cgaacataga taattttttg aataacgatt ataatcgcat attgttactt
     8101 attgattaat aacgatttat actttttgtt tgattttgca tttttgaatt tattttttat
     8161 tgaaaattta tgtggggaaa agtttgtaca tgccttttaa taagtgttgt agagcttagt
     8221 tgtttattag cttctgaaaa acctgaactt tcagattttg tatctaaatc aacttttaaa
     8281 gaaaacaggg ctgacaatat attgccttct tcaaatgagc tatattatta cagtttaaca
     8341 aacaatatgg tgatcaggta caaatttgct gatggtttat ctgtagatac ccctgtaaac
     8401 aatttaagta cagcttttga taaaaatatt gacattgcct ctttttcttt tgacaattca
     8461 gaacaccagt ttttgctggc ttacaacata aaaccattat acaggtattc attttatgca
     8521 tcgtatgtta tatttaattc actaaccaac gaatgcataa aggtgtctga agggaaaaag
     8581 attcgagatg cccaaatatc gccagatgga aataaggtgg cctattgtta caagcataat
     8641 ttatatgttt ttgatgttgt tcaaaacaaa acgacccaga ttacaaatga tggagatgga
     8701 gattccatta taaatgccga accagattgg atatacgaag aagagttttc gcttttgcaa
     8761 gcatttaaat ggtctccaga tagtaaaagc attgtttatt acaagttgaa tgaaaagaaa
     8821 gtaatgactt atcctttgac ctactataca aaaactaatt gttttcaggc agacgattgg
     8881 gtatatccct atgtggtaac cctaaaatac cctaaggtag gaaagcctat agctcaaacc
     8941 tctttatatg tatatgattt acaaacaaag actaatacca tgattgtcaa tgttggtgaa
     9001 aattatatta cagattttaa atggacaata aaatctgaca atgtatttta tactcgtgtt
     9061 aatagggctc aaaataaata caacatagaa gaatataatt ttgtaagcaa ggaaattaaa
     9121 gtactttata atgaacaaac aaatacaaat tttgaatttc cagatgttcc aagtgtgttt
     9181 tttatcaatg acaagaatga tttcgttttt ctaagtgaaa aaacgggata taaacatctt
     9241 tacttggcta cagaaaatgg ctcaaaaata aagcagttga ctactggcaa ttgggaagta
     9301 cttgcaatag aaaatatcga tattctaaat aaaagaatat ttcttactac taatcaagca
     9361 tcacctctac aaaagcactt atattgttat gacttgaaaa agttaaaatt atcgcagctt
     9421 actaaaggta atggtgtgca taaagctgtt tttagtgccg atttcaacaa tataatccat
     9481 acctattcga gcctgattga gcctttgaaa gttagcatta tgaatgtcaa gtctgggaag
     9541 gagaaggtgc tgattgataa taaaaaaatg gctgataaag caatagaaat gaatgttcct
     9601 caaaaagata ccttttcttt tgtacttcca agtgatgaaa gattgtatgg ctggatgata
     9661 aaagctaaga aagaaactaa ccgtcctgca cctactttat tttatgtata tggagggcct
     9721 ggtattcaga acgtacttga caaatatgaa gtaaaatggt atcattactt ggcgatgcaa
     9781 gggtataatg tagtttcagt tgataaccgt ggaacaagca acagaggtaa atcattcagg
     9841 gacgctactt atggaaaact tggtgaaata gcatgtacag atattgttgc aactgcaaac
     9901 tatctggggc aaaaggcttg gattgacact tcatgtttag ccattatggg atggagtttt
     9961 ggaggttttc ttgctgccac ttgcctgcag caaagcaatg gtttatttaa agctgccata
    10021 tctgttgctc ctcctaccga ctggcgtttg tacgatgccg cttataccga acggtatatg
    10081 ggtttgcttg aagacaatga agaaaagtac aataaaacct ccttgattgt aaatgctgat
    10141 aaaactaatg gtaagctatt attgattcat ggtactgctg atgacaatgt acacgttcaa
    10201 aatacgctga tgtttgctga tgccctggta aaagcagaca aaaaatttga aatgaaatta
    10261 tttactgacg acaaccacca gattaatagc caaaatgccg gaatgcaatt atacaaccag
    10321 gtgatagatt ttttaaacag ggtgtttgac aaataagcta tttaatttta tgcgttttaa
    10381 tgtaatttta attattgaaa tattatgaac ctcataattg acataggtaa cagccggata
    10441 aaacttggag tgttcaagaa taatgagctc ttagagtctt ttgtctttgc agatatgttt
    10501 gtatgtgatg ctgagcttgt acttcgtaag tatagagata taaaaaactg tattataggg
    10561 gctactggaa ttattccgac agatttactc gaatatttga gcaatacatt tcctaatctt
    10621 attgttttat cgggcaatac taatcttcca tttaagaacc tttatcaaac caaggaaact
    10681 caaggtcctg acagaattgc tgcagtagca ggagccatat caatgttccc aagctcagat
    10741 gtgttggtta ttgatgcagg cactgctatt acctatgact ttattaataa gcaaggtgaa
    10801 tatcaaggag gagcaatagc tcccgggatg acaatgcgtt tcaaggcctt aaatagtttt
    10861 acgcagaaac tacctctcat agcagccgga gaaaaagccg aactgcatgg aaagactaca
    10921 gaagagtcca tcaaatcagg ggtacagaat ggcattatat ttgaaataca gggatttatt
    10981 gagaaacatc gtttggataa tcctgaatta aaaatagttt tgtcgggcgg cgatgccaat
    11041 tttttggcag aacaaattaa ttgcaccatc tttgtagaac aaaatatcgt tttaatagga
    11101 ttgaacagta tattgaatta caatgcgcaa taaagtatat atagttttat ttataagtat
    11161 agtatttatc acttcttcat gtgagaataa cattgaagtc attcgcaaca tgggaaaaga
    11221 aaaagacttg ccacaggtaa gtgccgataa tattgaaatt ctttatagcg attcggcaag
    11281 ggtcaaaatt caaatcattt cgccatcaat agtgcgctat aatttgccca ataagatgta
    11341 caccgaattt tcaaagggcc tgaccgtcaa tcaatttgat acggcccata atattgaagc
    11401 cactattact gcccagcatg cattgtaccg cgatcaggaa aaactttgga aagcctgggg
    11461 caatgtattg gccagaaatc ataagacagg tgaggagctt agcactgaag aacttttctg
    11521 ggaccaggaa aagggaatta tttattcaac aaagttttcg agaatcagca atgcagacgg
    11581 ggtattctat gacgatgggg ggtttgaggc caaggaagat atgtcgcaat ggcacatgat
    11641 aggtataaaa ggctcggtaa atataaaaga taacggcaat gagaactaaa atccgcgaat
    11701 atgtatggct ctcattagca atattttgta ttgcggctgc cattcatcaa actttcaagt
    11761 taggattttc tgaaagctat gttttcttta tatttgctgt aatggcattt ttgatatatt
    11821 acatacgccg gactttacgt ttagctaaga aagactaaca aagcaaaact attatttcta
    11881 attattaact ggaatataag caaatattga aaaaaataag ctgtaaaatt ttacctctta
    11941 acctctttca aatcttctgg taaaaaagta attatttcta ttagtcctgc caaccgtcca
    12001 ttttcaaacc agggagtttg gtgaaccagc ttgcgtttgc cttctttttc aaccatgtat
    12061 gtattgcttt tcttatgctc aagcaattcg cggatgatac ggttcgagtt ttcgttgtgg
    12121 caatcgtaaa ggcttttgcc aatgaggttt tgcccgccat atttgtttaa catttgacgt
    12181 gatgctgcat tcatgtatag aattatacca tttgcatcac agactgtaac agggttttct
    12241 acttcttcaa aataattaaa catcatatat tttcttttaa acgcaaaggc acaaagaaaa
    12301 acttagcaac ttagcgcctt tacgttaata atttatccca ttattactac aatctcattg
    12361 ttgctggcaa ggtcgctgtt ggcaatttta cagccatcaa tggttttgtt gttgatgttt
    12421 atttttgaaa cacctttttg aacgccgcaa gggttcttca caataatgtt caattgctta
    12481 cctcgaaaac gacgggcaac tttaaattcc ttccagtcgg cagggataca aggatctatg
    12541 ataatcccat cataatcagg acgaacgcca agaatatact gcgttgctac aaaaaacgac
    12601 caggtagcag agccactgag ccatggcaaa cgtgatgccc caaaacgagg actatattgg
    12661 ccatgtgttg actgattgta cacgtatggc tctatttgtc gaatttcagc acgtgtattg
    12721 tatgctgcag gcatataagc cctccagtac tgataagccc tgttaccatt gccaagtaat
    12781 gtctcggcca tcacagccca accttgtgta tgggtgaata tagcaccgtt ttccttcata
    12841 cttgggttga aaagagccga gcggacaatg ttgtagtctg caccataagt gtaaggcgga
    12901 ttgcacaaag caataccata ctcggttgcc aacatttcgt tcacgctctg catagctgtt
    12961 tttgcctgtt cagcagaagc tgcttcacta attacagccc aagcctgagg attgagatat
    13021 atcttacctt ctttgctctc gtttgagcca aatttgaaac catctacacg atatccgcgc
    13081 aggaaccatt tgccatccca aacgtatttc tgaatatttt catcaatctg ggcaagttta
    13141 ggtttagccc attcaacttc ctcagatttt cctagcattt tgcaaatctg aatatattct
    13201 ttcaaagcaa aacgcaactg catagccaca aatgcggtct ctccatcatg tccaaacttc
    13261 agacagtcgt tccagtcggc cagcaaacca caaggtaagc catgtttacc tgacctgtct
    13321 aaattgaatt gaatagctct tcgtaagtga cctaaaacag tgtcttcgcc tttgtctgca
    13381 taaggaagca ctttattgta gaagtttata tcacccgttt cttttacata agcaggcaca
    13441 gcattgaaca accagaggca atcgtctgaa cggtactgat tttcatcagg taacttttcc
    13501 ttacccggct tatgagctat tttgttgacc acaggcatag ccccacctgt agaaacctga
    13561 ccagttatca tcagttcaag acgcttacct gcctcatctg gaatattagg caaaacaccc
    13621 aaaatatctt gtacactatc acggtagcct aatccgtcac gttccatagc tgtatatacc
    13681 aagctggctg cacggctcca gttgaatgtc atctgacagt tatacgggct ccacatattg
    13741 agcatgctgt taaattcagc atccggagtt tcaactgcaa cagcatctat acggccatgc
    13801 cagtagcgga taagcttatc taattcctgt tcgagacggg caacattgcc aaactcagca
    13861 actacctttt taccctcgtt agctgcttct ccaacaccaa gcagcacaat cagatcctgt
    13921 gattgacccg gagccagttc aatatctatt tgtgttgtac cacaaacatt gtcgccagca
    13981 gccataaaat ttgaacattt accagtttca acagccagtg ggtttgcata agtgcggtat
    14041 ctgccaagga atgattccag gtctgtttcg tgacccacaa ccttaccacc aacaaatgca
    14101 aggaatgtat ggcgtccctg gtcattttcg gcaaagttat ctggcattgc aggaatatgc
    14161 acgttggttc cgtggtcaat gataccatcc accacagaca tctttgcaat gtactgtgta
    14221 tattgaaggt tcaaaaggtc ttgcatagca ttccagcaat tggcaaactc tacaaacgag
    14281 aacatgctca atttgcgggc acgtttgtct ttgttggtta ccttgatttt ccaaacctca
    14341 aaaatttgtc caagtggaac aaaataggtt gtttcggtag caatgttatc atactctgaa
    14401 ctaataatgg tatatgccga accatggcgg cactctgatt tatatttatc aagcggtttg
    14461 ccaacaggct gccatgaagc tgaccaataa tcagcacttt ctttatcccg gatgtacaaa
    14521 tatcgtccag gttggtccat tggaatagag ttgaaacgga tacgcatgaa acgtccaatc
    14581 cctcctgatt tgtaaaagct gtaccccccg gcattattgg taataatagc accatattcg
    14641 gtagagccaa ggtaattgct ccacgaacgc ggggtgtcag gtcgagtaat tacatactct
    14701 tttcttttgt cgtcaaaaaa tccgtactgc atagtgtgtg tttaaaaaaa taactaatga
    14761 caaattacta attaaaagtg aattataaat ttcgttaaaa ttcatgaaca tgtcaagtct
    14821 aaaatcagtt aaatgatttc aggctgcata attacaatat tttttgaaaa tgatttctta
    14881 aaaatcgaaa gaagaattat agattttaca cttttcattc accttttttc ttgataaaaa
    14941 aggtgaccaa aaaatcaaga tgattgaacg ctatacggtt tttcattggt tagtcagtgc
    15001 ttcgtaggaa cgctgacaat catcactttc taataaaggc aagccttcga aaaatccctt
    15061 atttaaaatc aatacctgta aataaatcag gttcattgtt taaaatacca aaagacttag
    15121 atgtagaagc aatttcctgt attccttcag taatcacctg atcttcaatt ctatttaata
    15181 aaaaatcgac aaaatcattc acatccttca cttttgaaac tggaagcttt ttaatattgt
    15241 ttatagtttt attaatcaat atgcttttat ctattgactt agattttatt gaaagataca
    15301 aaattgatat tttgaatgct cgccaaaaaa ttattaaatc tcaattcttt tattcctgct
    15361 catccgcatc tgcatcatcc tcgttgatac cttccaatgg caatgcttca tttccgccat
    15421 tcatcagctc aacaccttta agcttgcgct gtatagctcg tgtacgagtg ctcataacag
    15481 tatccaactg gttcaaacct gtttgaatgt tattttgtgc tttttgcagc atgcctccaa
    15541 aggaatcaaa ttcctttttc acagcagcca gaacctgcca gacctcacta ctgcgctttt
    15601 ggatagccag cgtgcggaat cccatttgca aactattgag aatagctgcc agcgttgcag
    15661 gacccgttac cacaatttta tatgtcttct gtaactcctc aagcaaactg gccttacgca
    15721 caacttcagc atagatgcct tcaaaaggta agaacataat tccaaagtct gttgttgacg
    15781 gaggatcgag atatttgtcg ctaatatcct tagccatttt cttgatggtt tgctccaggt
    15841 ttttctgggc tgtttctatt tgcgaagggt ctccattatc atacgctgtc tgtagctgtt
    15901 cgtacacgtc tttcggaaat ttggcatcga cagggagcca tacgttgcgg tggttatcat
    15961 ctttaccagg caatttaaca gcaaattcaa ccacatcagc agaaccttgt ttggttttta
    16021 cattagcttc gtattgctca ggagcaagaa tttgctcaag aagcatagat agctgcacct
    16081 ctcctattcc gcctcgcatc ttgacattgc tgagtacctt tttcaggccg cctacatcct
    16141 gtgccaatgt ttgcatctca cccaagcctt tctgcacctc aataagctgt ttaccaactg
    16201 tttcaaaact ttgtccaagt ctttcgctca gcgtcttttc cagtttttct tccacggtaa
    16261 cacgaatggt ttcaagcttt tgctcggtat tctttaccaa atcctgctgc tgaacctcaa
    16321 gctttccaaa cttctccttc tgaaggtcat taaaagactg tacattctta tcaaatgtat
    16381 cctgaaaccc tttgaatacc ttgtctaaag tttcacgcaa ctgctgattt tcaagtttag
    16441 cttggttaaa aatatcttta aactttgcat caagcaccaa attaaggtct ttctgcaatt
    16501 gattattctg ttcaataata gtcttgaagg tttgagaaag ctcagccctg aattccttca
    16561 gcgtattgtt cagttcctcg cggttttcct ttgccaacgc agcattctcg tcacgattgg
    16621 ttttaaactc agaaggaagc ttttcttcca acttatttac agaaaattgc aactcttgca
    16681 gtttctccag ctcaatttta ctatcctttc cggaagtttt gttgagcaat tgcaataaaa
    16741 taagtaccac aaacaataca atacagacag ataaaagaat agtcgtcatt tgttacaaaa
    16801 ttgagaatga aaattttaca ttatttaact tcaatagcca aagttgcaaa aaactttaca
    16861 accgtatggc ttgatttttg aaaatataac ccaaaatttt gaacaattct caaacgttaa
    16921 tatttattat atgttgaata ctgagccaaa acgttcggtt tattttttat acttttgaat
    16981 acttaaaaaa tatagatgca tgataacaac cagaaaaata ggcattttcg caacattgtt
    17041 attgacggca gttactgtaa aagcaggaga ttcgcttcag tcgatattac aaaatgagct
    17101 tcagcgtgag cttagcgtat tgtccaaaca atcgctgccg gcatattata tacaatatag
    17161 agttgacgat attaaaaatt atagcattgc tgcatctttt ggcagtttaa cagatgaaga
    17221 ctctgacaac agcagccttc taaacgtaga agtccgaatt ggtgattata ccaaagacaa
    17281 cacgcacatt acaaaacttt ctgatgaaag gccttctatt ccggtgactc tggcattgcc
    17341 tattgagaaa aacaaggaag ctattgaaat agctttatgg caagctacgc attatgctta
    17401 tagaaaagca gctgacgagt ttaacaacat tcaaagcggt atgctaaatg atgcagaaga
    17461 aggtactgct gatttttcaa aggaaacacc taatgtatat tatgaagccc ctctggattt
    17521 caacaaagaa aagctttcgt acatgcgtga acggatgaaa aatatttctg gcttttttac
    17581 ttcaacacct gaaattcagt cagcagatgc aaggttttcg tatgtttatc aacgtaaata
    17641 ttttgccaat actgaaggta cagacattgt tcaaaacaac acctattgtc aaatacaggt
    17701 tgtagtagta tcaaaagctg aagacggcaa ttatgttcca ttggtaaaaa catatagtgc
    17761 ttttaaccca gatagtctgc cttctgaagc tactatcatg caagatatgc agcaaatgtt
    17821 aaatttgcta aagcaacttc gcaacgcacc tgtggccgat gcatattccg ggcctgctat
    17881 cctttcacca gatgctgctg ctgttttctt tcatgaaata tttggacacc gcattgaagg
    17941 gcagcgtatg aaaaacaatt ttgacagcca gattttcaaa aacaaagtag ggagccgcat
    18001 catcaacaag catcttactg tggtgtctga ccctactatg aaaaaccttg gaaaacttga
    18061 catgtttggc tattacaaat atgatgacca gggaataaag gcttccagag ttgagcttgt
    18121 caaagatggt gttttgaaag attttctcat gtcgcgtact ccaataaaag ggtttgagca
    18181 ttccaacggg catggccgct ctcagacagg attgtctccg gtttcgcgtc aatcaaatct
    18241 gattataact tcaaatcagg tacattcaaa agaaaaacta cgtaaaatgt tgattgctga
    18301 atgtaaaaag caaaacaaaa catttggata ctactttgac caggtaattg gaggtttcac
    18361 ccttaccaac cgctttactc caaatgtttt caatgtaagc cctgttgttg tttaccgtgt
    18421 ttatgtagat ggccggcctg atgagttagt acgtggaata aacttcattg gcacaccatt
    18481 ggccattttt gaaggcatcc ttgctgcagg tgaccaatat ggtgtattta ccggatattg
    18541 tggtgccgaa tctggtagta ttcctgttac agccagtgca ccatctctgc ttatcaagaa
    18601 aattgaaacc cagaaaaagc cagaaggcct aaacacaaag cctattttgc cacgtcctga
    18661 ttttattaaa gaaaataagt aaaactatga gtaaatatat caaacatata tcaaccccaa
    18721 tattagtcac tttgctgact acaaccatct cagcccaaga tagtatatca aatctgattt
    18781 tcagggcaat gaaagatgaa attgaccgta gtataagcca attagaatat aaggattatg
    18841 caaagccgtt ttacataagc tataccattg aagataccag gactttatac gttaatgctt
    18901 ctttaggaag tattgttaac tcgaacgaga agaaataccg ttcatggggc aacagggtat
    18961 tatgtggaac ttatgagctg aacgatgaga attttatgga tgccacacgt aacccggcac
    19021 catctgatgg acgtttgcaa ttgcctatag atgacgatta ctggggtatc cgccgtgccc
    19081 tttggattat tactaataat acttacaaat ctgctgccga aaattaccgc agtaagcttg
    19141 aagcccttaa ggacaagaat ctgacaaaag acacactgga aatacctgat tttgcaaagt
    19201 ctccaacagt aaacctaatc attgagccta aaaataagga atggaacaaa caactgttgg
    19261 aagataaagt aaaaaccata tcttcaacat ttttaaaata tcctgatatt gtattctctg
    19321 acgtttcaat ctatcaaatt aatgcaaacc tatattttta cagttctgaa aatagtaaaa
    19381 ttaagcaacc tgttcaattt accatactaa cagttacagc agtctgcgaa gccggtaatg
    19441 gagagaaatt ttcaaaccag cttcaatacg ttgtaaacct cccatctcaa ttaccttcaa
    19501 acgatacaat tatcagtcat tgcaacgcct tggcaatatt tcttggccag ctgcaggatg
    19561 tcccggtttc aaaagaaaat tattcaggcc cggtaatgta tacaggacaa gctactcctg
    19621 aaatacttac ccagtgtatg ttctatggca atgacaatct gtttgcctat cgcgaaccac
    19681 tttacaacaa ttctcaaatg tcaatgtatt acgggcaaaa catgaattcg tttgaaacaa
    19741 agcttggcaa attggtagct tctaaaaaca tttccattac cgaatttaca aaaaaagaca
    19801 attaccatgg catccccctc atcggcaact atcaatgcga tgcagaagga gtaattccta
    19861 aggatacaat ggtacttatc gacaaaggaa ttctaaaaac attatacaac ggaaggaccc
    19921 caaccagaaa tatcccatta tcaaacggac acagacgcta tgtattggta aatggaggta
    19981 ttagcaacaa ccttggcccc ggagttgttg aaattagctc tagcgaaaag aaaagcgtga
    20041 ccgagctaaa aacaatgctt atcaatgaag ctaaaaatga aggactggat tacgcaattc
    20101 ttataaaacc tattgttgaa acaaacggat atactcctct tgccgcattt aaggtagatg
    20161 ttaataatgg tagcgaaaaa cccatcaggg gattgagcat gaaaccatta aatatcaact
    20221 atttgaaaaa aatcataggt ttttcagata aaaacattgt ttataataat attagcgaag
    20281 gcggcatgtc tccaatagat ggcagtaaca gcggaaaaca agtaatacct tccgggaacc
    20341 ctaccagcat tatatgtcca tcagcaatag ttgtgaaaga aattgatttt gaatacattt
    20401 caaaagccct cgtaaacgat aagataatag ttccaagccc tttgaaaaag taaaccaaag
    20461 ttttccattt aacaatattt cttaaaaact ctttaataca cttatttgag tttttatagg
    20521 agctattcaa taaaagccct gatgactatt aaagtctttc aggagttttt cattaacaac
    20581 ttttaaaaaa atctttaaca tgccttaaca ctgacaattt ttcattcggc atataatttg
    20641 tcaaatttgc agtagaaact tttaaattcg aataatatga aaacttttaa atattcagga
    20701 atatttattt ctgccctggc tggagccata atggcagtat tgatatcgaa ctttttcttc
    20761 cctaaaaaac aaattgttta caactacccg ggatataaag cccagaatgt ttcttatgac
    20821 ccgggggcag ctcagccaat tgactttact atagcagccg aacaaacagt gcatgctgta
    20881 gtaaacgtca ggactaaagc caagaacgaa aatcagtttg ttaacccgct gtattacttc
    20941 ttttatggac aagatgaacc tgtacaacag cccccaaaag ttggtattgg ttctggcgta
    21001 atcattgatc caaacgggta tattgttacc aataaccacg ttattcaagg tgcaaacgaa
    21061 attgttgtca ctctgaacga caaccgcgaa ttcaccgcaa agctaattgg tgctgacccc
    21121 agtaccgacc ttgcattgtt aaaagtcgaa gccaacaacc tgcctgtcat cccatacgga
    21181 aactcagaag agcttcgatt aggccagtgg gtattggcta ttggtaaccc atacaattta
    21241 tcttctactg ttacagcagg tattgtaagt gccaaagcca gaaacctcaa tattatcaac
    21301 gacaattaca gacttgagtc ttttatccag accgatgctg cgttaaaccc aggcaatagc
    21361 ggcggagctt tagtaaacac taaaggcgaa ttagtaggtg tcaacaccgc catactttct
    21421 gttacgggcg gttattctgg caattcgttt gcaatacccg taaccattgt aaaaaaagtt
    21481 gttgacgact tacgcgaatt tggcgcagtt cagcgtgcat ttcttggtat taccccggcc
    21541 gatgttaatg ctgaaattgc aaaacaaaat aacttaagca ttacaacagg cgtttttgtt
    21601 gctgatgtta aagaaaccgg agccgcaaaa gaagccggaa taaaagcaaa tgatgttatt
    21661 actaaaatta ataacgtccc ggttgtaaat acgtctgaat tacaagaaca acttagcaaa
    21721 tacaggcccg ggcaaaagat tagcgttact attctaagaa atgacaacct taaagatata
    21781 tcagttacat tacgcaacct ccagggtgaa acaaaattgc taaaacctaa tgatattact
    21841 actacaatta tgggggcaac actgtcagac attactgaag aagataagaa aaaccttgga
    21901 attaaatatg gggttaaaat tgtctccctg tcaccgggaa aattttctaa agccggtatt
    21961 gaagaaggtt ttatcattac tcgcttaaat aataaagctg tcacatctgt tgctgaaatg
    22021 aaaaaaatca ttaacaatac tcgcggaggc ctttacattg aaggtattta ttcaaatggt
    22081 gttattgctt actacgcatt tggattataa actcaaaatt ttgaaatctc tacaatttaa
    22141 aataaaatca gttctacata agaagaagct tgcatatatg atatatacag gaatcaacta
    22201 tacataccta acttttgcct tgaagcaaaa gttacaaaaa ttcaaggctt gacgtaaatt
    22261 ggctaaaaaa agctttatcg acggctccgg gcaacaactc gcttctctca aacaattgcc
    22321 ctcgctacgc cgataaagct tttttcttaa cgccatttcc gtcaggccat aagcagaagg
    22381 gttcccatga aataggcgaa gcgataaaaa gaaaaattcc ttttcatcgc ctttttgttt
    22441 gatttcggcc gagaagcctg acgcttcagt tgttcgcata ttacaacatt acaaggcttc
    22501 gagggtgaaa gagttaagcc gataagagtt tttgagcaac ttttttcgat aaaaaagttg
    22561 catgaagatt atagtattta ttgctattca atgcttttaa aacaataatt gacaatatta
    22621 ttctttcatc caactgacgt taaaatactg tagtatttcc cttccttttc aaagagagct
    22681 tgtcccgtct caggcgggag gctggagtgg ttaatacccc gtgttgcatt tttgacagat
    22741 tgttaataac ttactttctt aatcaccaca tttttttttt agtagcgggc taatgcttaa
    22801 ctttgcagca taaatatata ttgtatgtta aaatttttca aaggccctga aaacaagtat
    22861 ttcacagtac attttgccac agaccctgat gcaaaagatt tacaaaagct tagctggtta
    22921 tttggcgcaa ctttggttga ttctgaaaaa gttgacggtt ttttcattgg gccgcgcaaa
    22981 gaaatgatta ccccatggag tactaatgct gttgaaataa cccaaaacat gggaattgaa
    23041 ggcatcaaac gaatcgaaga attctaccag gttgaatctg ctgataccaa atatgaccac
    23101 atgttgaaag ccatgtacaa tggacttaat caagatacct ttactgtttt gcgcgaacct
    23161 gaacccgtca aatacattac agacatcaga acatacaata aacaggaagg tttagccctt
    23221 aatgatgatg aaattgaata tttagaatca ctgcgtaaaa aattaggcag agaccttacc
    23281 gacagcgaaa tatttggatt ttcgcaagtt aactcagaac actgtcgtca caaaattttc
    23341 aacggaacat tcattattga caataaagaa aaagaagaaa gcctttttca actgattaaa
    23401 aaaactacaa aagccaatcc caatcaggtt gtttcggcat ataaagacaa ttgcgctttc
    23461 attcagggcc ctgttgtcga tcagtttgcc cctgcaaccc aggacaaacc tgactttttt
    23521 gaaacaaagc cttttgaatc agttatttca ttaaaagcag aaacccacaa cttcccaacc
    23581 acagtcgaac catttaacgg agcagctaca ggttctggcg gcgaaattcg tgaccgcatt
    23641 ggaggaggaa aaggttcgtt ccccattgcc ggaacagctg tttacatgac atcttatcct
    23701 cgttcggccc aaggccgtat ttgggaaaac aaccttagtg aacgtaaatg gttatatcaa
    23761 acacctgaag atatccttat caaagcatca aacggtgcaa gcgattttgg aaataaatat
    23821 ggccaaccac ttatctgtgg tagtgttttc accttcgaac attttgaaaa ccatcgcaaa
    23881 tttggatttg acaaagtcat tatgcttgca ggcggggtag gttatgccaa aaagaaagat
    23941 agcctaaaag ataccccgga accaggagac aaagtagtac tccttggagg cgacaattac
    24001 cgtattggaa tgggaggagg agctgtatct tctgttgcaa ccggcgaata caataacgct
    24061 atcgagttaa acgccgttca acgttcaaac cccgaaatgc aaaaacgcgt atataacgca
    24121 atcagggcat tatctgaaag tgatcataac ccaatagttt ctattcatga tcatggcgct
    24181 ggcgggcacc taaactgctt gtcagaattg gtagaagcaa ccggaggaaa aatcgacatt
    24241 agtaaacttc ctgtgggaga ccctaccctt tcgaataaag agattgttgg taacgaatcg
    24301 caagaacgaa tgggccttgt gctaaaagaa aaagatatag cccttttgaa gcgcattgca
    24361 gaccgcgaac gagccccaat gtatgtcatt ggcgaagcta ctggtgacaa tcggttcacc
    24421 ttcacagacc ccaaaaccgg caaaactcca atagaccttg aattggacga tatgtttgga
    24481 aatcccccaa agacgattat tacagataat tcgcaagtaa tttcatacgc agatgttaca
    24541 tacgatactt caaaacttga agaatattta gtacaactgt tacaacttga agctgttgct
    24601 tgcaaagatt ggttaaccaa taaagttgac agatctgtaa ccggtcgagt tgcaaaacaa
    24661 caaacagccg gagccattca actccctgta aataatgtgg cagttgttgc tatggattat
    24721 aaaggcaaaa aaggtattgc cacttcatta ggccatgccc cggcagcagc ccttatcaat
    24781 gcttctgccg gttctgtact aagtattgcc gaatcgctga caaacatcat ttgggcacct
    24841 ctggaacatg gcttgaaagg tatttcgtta agtgcaaact ggatgtggcc ttgtaagcaa
    24901 cccggtgaag atgccaggtt atatgaagca gtaactgctg caagtaattt tgccattgaa
    24961 ttaggtatta acattccaac cggaaaagac tctctttcaa tgacgcagaa atatcctgat
    25021 ggagatatag tattatctcc tggcactgtt attatttctg ctatagcaca agtatctgat
    25081 atcagaaaag ttgttgaacc tgttattaat ccgtctgttg attcttctat tttatatatc
    25141 gatttatcta atgttagttt tgagttagga ggaagttctt ttgcccagat aatgaacaag
    25201 ttaggatcat atactccaac tattgcagat actgcatatt ttatcaaagc ttttgaaacc
    25261 atccagtctt tcatcaaaaa tgaaaaaata gttgccgggc acgatatttc ttcaggcggg
    25321 atgatcactt cattgcttga aatgtgcttt acctctgtca atgcagggct gaacattgac
    25381 ctttctgtga taaatgaaaa agacattatt aaaatcctgt ttagcgaaaa tcccggaatt
    25441 cttgtccagg ttaccaaaga aaatttagca tttgtacaag ctgaattaaa taagaataaa
    25501 atattattcc atgaaattgg gaaacctgcc aataacggta aagttaaaat caaaaaagac
    25561 gacaccgaat ttgaattcaa tgtagccgaa ttacgcgata tatggttcaa aacttcttat
    25621 ttattagaca gacgccaaag tggcgagttt aaagccattg aaagatataa caactacaaa
    25681 gcccaaccgc tcaaattcaa cttcccacct tcatttacag gaaagtttgc tgattttggc
    25741 ataaaccctg acagaaaaac caagtcgggc attaaagctg ctattattag agaaaaaggg
    25801 gttaatggag acagggaaat ggcttggatg atgcaccttg ccggctttga tgtaaaagat
    25861 gtccacatga ccgacctgat ttcgggtcgc gaaaccttag aagacctcaa tatgattgta
    25921 ttcgtcggag gcttttcaaa ctctgatgta cttggatcgg caaaaggttg ggccggggca
    25981 tttttataca atccgaaagc aaaagaaagt ttagataaat tctatgcccg tccagacacc
    26041 cttagcttag gggtttgcaa tggttgtcag gtggtcatag aattagggct gataacccct
    26101 gaacatgagc aaaaacccaa aatgctgcat aacgactctc ataaattcga atcatccttt
    26161 cttacggtag atattcaccc gagtaattct gttatgttaa gttctttggc aggttcaagg
    26221 ctgggtattt gggtagctca cggagaaggc aagtttagtt tgccttacga agaatcaaaa
    26281 tacaacattc caatgaaata cagttattct gcatatccgg gcaatccaaa cggaagtaat
    26341 tttgatacag ccggtttatg ttcagaagat gggcgccacc tggtaatgat gcctcactta
    26401 gaaagagcaa catatccttg gaactgggca aactacccgg ctgacaggaa gaatgacgaa
    26461 gtaactcctt ggattgaagc atttgttaat gcccgcaaat ggattgaaaa aaaagcaaca
    26521 acgcttaaaa gtaaataaaa tcaaggcaat gcagctattt gcattgcctt ttttattaat
    26581 ctaacattat cttatttat
//
LOCUS       DAOSPF010000049        26434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1839_length_26434_cov_8.412336, whole genome shotgun sequence.
ACCESSION   DAOSPF010000049 DAOSPF010000000
VERSION     DAOSPF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_26434_cov_8.412336"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..282)
                     /locus_tag="PK252_10140"
     CDS             complement(88..282)
                     /locus_tag="PK252_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15107.1"
                     /translation="MKKIALLSVAMMLVASLAVVSAQTTQTKSEPAKKECSKTKKEGK
                     DDKKAEKKAEKKEAATPEKK"
     gene            complement(675..1331)
                     /locus_tag="PK252_10145"
     CDS             complement(675..1331)
                     /locus_tag="PK252_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15108.1"
                     /translation="MKKLKVFIVDDHDMFREGVKVLLNKSKVIEIVGEAENGQEYLDK
                     VDKYSPDVTLMDINMPVMDGIEATRKRMAIDPDSKILTLSMFGDEEYYFKMIQAGVKG
                     FVLKSSGIAELENAITEVAKGEIYFSAELVKQIITNIKTDKAKIESAKDKDLLSNREV
                     EVLKLIAAGLSNDEIANKLNISVATVKSHRANLLKKTNCNNTASLVIYAIRNKLFVVE
                     "
     gene            complement(1459..2892)
                     /locus_tag="PK252_10150"
     CDS             complement(1459..2892)
                     /locus_tag="PK252_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15109.1"
                     /translation="MEKEIVLYIDDDETNLTLFTAIFENDYQVLTTSDTTEARQMMGN
                     NEIKVAIVDLFMPQESGTDFIEDVSKSYPDVFYIILTSHYDLDAALNAINKGNTYRYL
                     LKPWDTNEMRASINSAIFSYNLSIENKRLFNELKDKNTLLEKALTQLKERETQFLKIF
                     EASNDAIIIYNANKEILMANPVFYNFVQAKKGTRLKITDLLSDKLLEIFEQRFQEIFT
                     NPTPIFEYQLPAPNGEIRFIEANSTTINYYGEKVVLSILRDITERKLMAQRILNEVVQ
                     AEDRERKRLATDLHDGLGPTLSTLNMYIEWLADKTRKEDSEEILRLSRQTIKEAITQL
                     RSISYNLSPHLLEKFGLNAAVQSLIDTMNEKGEINFRFQSNLCERLPHNCESALFWVI
                     KECINNTQKHSKAKESFISLYLKNDLLYFDYADNGVGFDYDTVKKNSMGMGLYNIQNR
                     IQSLGGNSEIKTETGKGIKIKATIKIS"
     gene            3069..4907
                     /locus_tag="PK252_10155"
     CDS             3069..4907
                     /locus_tag="PK252_10155"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT15110.1"
                     /translation="MERNRSIIIRFLFALISISYSFFFIFKFIRPELYFHFQQPPFLL
                     TSGFFYGYLSYPGGFSYYLSDFLSHLFYFNFLGGLIIFVLGLLIYFLFYWTLRLKAKF
                     FMATIPVAMFFALLCNVYFPFSTIISFIITLSLILLTRNFSKNTSFAFILIIVGFLTT
                     YYLAGIGYTYVYAISSILALLSNKALKKRFLAGAVLVFIISALLYLLANYMFLLSPSE
                     MYWTFFRKLALFMSYKPTKFYYLFVFSPLILMAVDILAEWKKSHWTLEYLDKVKIIAV
                     ASVFFAIFISISLYFPYKWDLSKKRNFVLCDYYNEQGKWEKTIEVAKQSTEYDYLTNL
                     NFSRAIFNSGNSLNQLFDYPQVVGKDGIHPDYSYSGEIVFLSQDAYYDLGLISESRHW
                     AYESFIGFPYNTRALKALVKIHFINRQFDAAANCLDILEKGLISSDFVKKYRPYLKDT
                     NLVNNDPELAEKRKNMPTGFEISESLELKLNILLEKDSSNKKALEYLLAFYMLDSQLD
                     KFMSLVDYASQYYNQWPEIVQEAIVVYGAVRGKKVIKEYGISPNTVERFKYFSLTLRN
                     CGKDMDLAKDLLYPDFKNSYFYFFKFLNPKVTNAKIVVNLDNNSSI"
     gene            4914..6416
                     /locus_tag="PK252_10160"
     CDS             4914..6416
                     /locus_tag="PK252_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15111.1"
                     /translation="MNKYIWAIYIVLLLAVSCKPDVADFIDEKKEASIFPDYSGLVLP
                     ANIAPLNFVVKEKADKYFIRIKSSRGNTYDICQTSPKIDISERKWKKLLSESVNANLY
                     ISIFAKKDGKWYKYNDIKDSVVDKKIDNYLVYRVVYANYLFWRKMEVIQRDLESFNEY
                     TVYDNASGDDGCVNCHSFPKNNPNKMMMHFRVIHPGMLLNLNGQIKKIDTRTQYTLSG
                     AVYPAWHPDGKLIAFSTIKLSPHITTDIHKIIDASDKASDLIVYNTETNIITTSPKIS
                     TKSRENMPAWSPDGKYLYFIVAPQVENQDIKSLRKVKYSIARIEYNVNTNTWGNVDTV
                     LSASQTGISMSFPLISPDGRYLLFTGTDYGYFTAFHNQADLYCYDLQAKTLSKPALNS
                     SSCESYHTWSSNGRWIVFSSKRMDDVYTRPFIAFFDEKGNTSKPFVMPIKDPDEYSTL
                     LANYNRPELVTGKIELSNRQIRDAVFAEPVPAKFDTSVEVDALSGATAKK"
     gene            complement(6738..7472)
                     /locus_tag="PK252_10165"
     CDS             complement(6738..7472)
                     /locus_tag="PK252_10165"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOT15112.1"
                     /translation="MKYFRILVLLVLAICCSCKKEMFFDAGPEKSKKVLLESNFTYVE
                     VGKAFHIILISDSANYLVFKGGEKLIQEVSVNLYHDSLFISHHIGGHWYKLDDPMELE
                     IHVSSSPKFYFAAPVRVSTIDTFCSARFYMDFKSFCEINVTVNVSECAIFAPYDSFGN
                     FVINGKCDHAAFYASGSTLFRAENLQMQSCYVRQNSISEVYLNEPEQLSVLFCSSGNV
                     FCKKMPQNLSIQRQGIYSTGQVVEVK"
     gene            complement(7484..8596)
                     /locus_tag="PK252_10170"
     CDS             complement(7484..8596)
                     /locus_tag="PK252_10170"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOT15113.1"
                     /translation="MKHPKYILLTLFLILAQKGIGQVSALEKDFFMEGSYQYGYLLSH
                     KDYLAFFIHEPVQSFQVAFGKVVSGNKAWHQVFNYPSFGMGYYQGSLGNDQIYGHLHS
                     LYFFVDRYYFDVNGRFNIGNRLDLGLAYATRFYDQYTNPYNLALGSRYNVYARLCFEA
                     SYWATSQVRILAGAGFSHTSNGSFRKPNAGMNYFTTSASVQYHFREMPKRPRQTFVDT
                     DSAKNEFIVLTSIGRKNISRFDNKIFPLYSVNLAYNRKLYISRYVGLNLYLSYDPSIV
                     EKIHLVKADSVVTGNDQLRVAINASYEIRMGNLSFVFQPGIYLKNKIDVANNICNRLG
                     MRYYTNWGLVAALTLNAHWAAKADVVEWGIGYRFKN"
     gene            8733..9491
                     /locus_tag="PK252_10175"
     CDS             8733..9491
                     /locus_tag="PK252_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOT15114.1"
                     /translation="MDNTFNNDKKQLITAIPYKVTTANADMHLRLRWGALTDLLIQSA
                     IASADRLQAGFGQLKQQKLFWVLSRMTIEISNPLTLGQTGEVETWPKDVEKILYLRDF
                     VVKTHEQQIVAKATSGWLAVDMENRRPRTLEGDYSAIFSKLKDFHALQSQPEKLFAVK
                     EGEASEVKATYFDIDLNGHVTASRYIDWMMDTFPADFHRNHYPKTLSINYLNETLINE
                     TIRLTKATNDNKNFLFEGLNKDRNITAFRGKIGF"
     gene            complement(9848..10315)
                     /locus_tag="PK252_10180"
     CDS             complement(9848..10315)
                     /locus_tag="PK252_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOT15115.1"
                     /translation="MKKYTENHIVVPRFNEVNGFYTTKQRSELMGKIKSQNTKPELKL
                     KKALWNLGYRYRKNFKKLPGSPDIVYSKHKLAIFVDGEFWHGYNWSEKKTKIKTNREF
                     WIPKIERNIQRDKQNNQLLKDNGWHVIRFWENELKKDFDGCVNKVISYLQDGA"
     gene            10416..11648
                     /gene="dcm"
                     /locus_tag="PK252_10185"
     CDS             10416..11648
                     /gene="dcm"
                     /locus_tag="PK252_10185"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOT15116.1"
                     /translation="MEKYYSIAEVADLLSVSKETLRRWDRNGKLTAIREPMSNYRVYH
                     KDQLKIFDSLDFLFMPTESVNETKPTRDFYSIELFAGAGGLAIGLEEAGIKCLALNEF
                     DHWACETLRKNRPDWNVIEDDVRNVSFSNFKGKVDIVTGGFPCQAFSYAGKKLGLNDA
                     RGTLFYEFARVVQETMPAICIGENVRGLLTHENGRTIEGMISILDEIGYNVITPRIFK
                     TIFYKVPQKRERVLIVGIRKDLNPHLYEFPKPFKKVYTLKDALKKGELYNCDVPESAG
                     CKYPKSKKEVLDLVPEGGYWRDLPIDVQKKYMGGSFYLGGGKTGMARRMSWSEPSLTL
                     TCSPAQKQTERCHPDETRPFTVREYARIQTFPDDWSFSGSISQQYKQIGNAVPVNFAK
                     EIGYSIIRFLNKIYPKQY"
     gene            11709..12416
                     /locus_tag="PK252_10190"
     CDS             11709..12416
                     /locus_tag="PK252_10190"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HOT15117.1"
                     /translation="MPYLKWISDTDLEKEVDQLLSIAKDAKKAAIEEFGKNVIDPFAA
                     LFEMAGFEIDYDTWLKSETTRQAQKTLQNHIGNFHQNILGYCKGWSNKGVGNIIDLVS
                     LDNKIIAEIKNKYNTISGGKLSDLYYSLEKQVSPKSSIYKGYTAYYVAIIPKKGERFN
                     KPFTPSDKDKGEKCPINEHIREIDGASFYSLVTGSENALEDLFDILPEVIKLCSEGKY
                     QIKDKKKLKEFFNLAFK"
     gene            12858..13850
                     /locus_tag="PK252_10195"
     CDS             12858..13850
                     /locus_tag="PK252_10195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOT15118.1"
                     /translation="MKILIIQTAFIGDVVLATPVVEKLREKYPDSTIDFLLRKGNESL
                     LIGNPHIRKVWVFDKKNGKYKNLLKMAKELRTEAYDYVINLQRFFTTGLMTVLSGGKV
                     KIGFDKNPLSFLFTHVVKHHISHDDINVHEVDRNLSLIEPIVGDTAFKKPRLYPSSTD
                     YAVVPTGEEYICIAPSSVWFTKQFAEHKWIELINNIPAKYKVYLIGAKSDMPLCGRIQ
                     KATSHQSVEILAGQLTFLQSCALIDKARMTFVNDSAPLHFASAMNAPVTAFFCSTVPA
                     FGFGPLSDFSIVAEVEDKLDCRPCGLHGKKQCPQQHFKCSEMDVKKVLAKTKIL"
     gene            complement(13905..17816)
                     /locus_tag="PK252_10200"
     CDS             complement(13905..17816)
                     /locus_tag="PK252_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15119.1"
                     /translation="MKFKLQYCIQLFVIVLFVNSLHAQKTFKKFYHITRNSGLSQSTI
                     NTIVKDRTGFIWFGTDDGLNRYNGYTVQHFKHDENDYTTIGLGRVHCIYISKSGQMWV
                     GTDQGGLDLYDENTERFVHFKSGIEHVVAAATNDIRGILETADNRLWLGCYEGSISLF
                     DPKDKSFRSVPLSNKNNVTAICYDNDSNLYIGTTSGVEIIRNAKNGYYANIKSEPFGL
                     LKNINIISIYRASDGTMWFGTYGKGAFAYTPSNKKMLQYTTSSPGPYRIGHNIVRAFT
                     EDKNKNVIIGTGGSGLNILDENKHVCQSIKNQLNNQFSLGSDVIYCFLMDEMHNLWIG
                     TYNAGVDVVFEVKDKFGHIRSFGEENSLSANSVLTMLEDKSGNIWIGTDGGGMDKYNP
                     RENKFEHFRNNPNNPNSLSGTVVKSLMFDKDSILWIGTFNEGLNAYNPKTRKFTRYYH
                     DPANPNSPASNHVWDIDQDEDGMIWIATLNQGLDCFDPKTRKFTHFLHNPKDSSSLSD
                     YGVSVIEFDKNGNLWVGTEFGGISVMKKGEWGKFQNFVRSDNNNSALTSNQICSIFED
                     SKGRIWVGTLGGGINVYDEDVEKFYKLTVKDGLASNLVYAFLEDKEGNIWISTNNGLS
                     KLKYSENNIQYPFFQNYDLNDGLLSNEFSPQSAMMARSGLLYFGGINGINYFMPESLK
                     INTNVPPVVLTDFRIFNKSVAVDEPGSPLKVSIIKSKIIEIDYSQSVITFEFAALDYV
                     MPSKNRYQYKLEGFEQNWNDVGNQRTATYTNLNPGKYVFRVKGSNNDNVWNEDGASVL
                     LVIRPPFYKTWTFWVILITGFVVLTYFVVKERLTKFEKQQRSLKIMVDTRTKELVELN
                     EMLEIQNQEIDYQRAEVLAQKENLEMVNKALEEKQNQIQQQNIELEKHRNHLEELVAQ
                     RTAELEVAKQKAEESDRLKMAFLSNMSHEIRTPMNAIIGFSSLLNAPDITEKEKEDFI
                     RLINANSEALLVLIDDILDLSRIEANQIEIKKEVFNVSTFIHDLILSYRKIKKPKESV
                     DFYLNLNISGEKLIETDRHRLRQVLLNLIDNAFKFTPAGSIELKVEESNGEYIFAVKD
                     TGIGIDEASQAIIFDRFVKGHDDNKKVFRGAGLGLAISKSLMELLGGSLEVQSKLGEG
                     STFYVKLSSQLSVQKPFTADQKEMQTISVINPVFTDKVILLAEDEKGNYLYLSKLLGQ
                     QGAIVHWAKNGIEAVDMCRKNKYDLVLMDIKMPDMDGIEAFKIIRAEKPELPVIAQTA
                     FARHEEEQNIRAVGFDDYISKPIRKEILLDMIKRVLSKKQE"
     gene            18113..20221
                     /locus_tag="PK252_10205"
     CDS             18113..20221
                     /locus_tag="PK252_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOT15120.1"
                     /translation="MKKIFLLLIAYAWTQLNAAELKSPNGQLSLNVTVIDSVATYRLD
                     YKSRPVIKQSRLGLELKKDKDLIHGFVISKVDTSTFDETWQPVWGEVKNIRNYYKELA
                     VTLSQPSTKRSIIVRFRLFNDGLGFRYEFPQQDNLNYFIIKEERTQFAMTGDHKAFWL
                     PGDFDTQEYSTVTSKLSEIRSLMKSAITSNASQTQFSPTGVQTSLMLKSDDGLYINIH
                     EAALVDYSCMSLNLDDKNFIFESFLTPDAIGDKGYMQTPCKSPWRTIIVSDKATEILE
                     SKLILNLNEPCQYSDVSWIKPVKYVGVWWEMITGKSSWAYTDVNSVKLGEFDYNKAKP
                     NGKHAANTAHVKEYIDFAALHGFDAVLVEGWNIGWEDWFGKWKDYVFDFVTPYPDFDI
                     KEISQYAASKGVKIIMHHETSGAVRNYERHLDTAYKFMVANGYNAVKSGYVGDIVPRG
                     EYHYGQWMVNHYLYAIKKAADYKIMVNAHEAVRPTGLCRTYPNLIGNESARGTEYEAF
                     GGSNPDHTTILPFTRLMGGPMDYTPGIFETHIWKLNPDNKSYARTTLARQLALYVTMY
                     SPLQMAADLPENYNRFLDAFQFIKDVAVDWDDTRVIEAEPGDYITIARKAKGTNNWFV
                     GNTSDENGHLSKFKLSFLDAGKKYKATIYADAKDAHYEKNPQAYTIKTIDVTNKTELS
                     LQAAPGGGFAISIVEVPAKK"
     gene            20442..21767
                     /locus_tag="PK252_10210"
     CDS             20442..21767
                     /locus_tag="PK252_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HOT15121.1"
                     /translation="MKIFSATIFLAFVMFFTSFGQTTTATDTCKNDVPDQRGIMKPWE
                     KGAYETGKYRNVFLEAGYKQADIDAKLAKAYYDVFEGPKRVYFQVGDSMGYVSDVKNN
                     DARTEGLSYGMMVAVQLNKKDVFDRIWRWSKKYAQHQGGPRDAYFAWSLDTATGNKKF
                     AGSASDGELYFVTALLFASNRWGNNTGINYYAEARRILDAMWSKNGKDGITNLINVEH
                     KQITFVPDTAGYKWTDPSYHLPAFFEVWAMYAKDGHEQFYRDCADTARAFLHRACHPV
                     TGLNPDYANFDGSPKRVWKVMGDAFRYDSWRVPMNIAMDYSWFAKDKEWQKDYAVRFQ
                     NFLFCKGIDTFEDQFNLDGSRPNWILGAGTARIQKLRHSLGLVSTSAAASIMGTQAKS
                     WKFVDALWNAKLEPYEDGYFDPYYDGLLYLFSLMHLSGNYRIITPVGTK"
     gene            complement(22124..>23010)
                     /locus_tag="PK252_10215"
     CDS             complement(22124..>23010)
                     /locus_tag="PK252_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HOT15122.1"
                     /translation="FDISEVVECTIEVNPDDINAEYVKGLKSIGFNRVSIGIQSFDNG
                     LLKLMNRRHSKEDAIKAVYEFYNAGINNISVDLIYGLPGTDEIYWENTLLEAFKLPMV
                     HLSAYCLSYEEGTVFYNWYQRNKIQQIPEEMQWKQYQLLHEISMNNGFEHYEISNLSK
                     PGYKSKHNSSYWQGVSYLGLGPAAHSFNGHARSWNIADIGQYIDLIRENKICREEENL
                     SESDQFNEYLMTRLRTSDGISIAEMKQKYQPIFTNDILNEIKNRYSQYFESINEERIA
                     MNLEGWFVSDSIIAGLMK"
     assembly_gap    23011..23110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23111..23496)
                     /locus_tag="PK252_10220"
     CDS             complement(<23111..23496)
                     /locus_tag="PK252_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT15123.1"
                     /translation="MLSNTCKYAIRACIYLALNADEEKKIGIKKISDDLKIPMPFLGK
                     ILQSLAKSKVLLSTKGPNGGFCLAKPAKKISLMEIVDAIDGDESFTSCVIGVTSCSTS
                     EAHCPIHEKYGPLREKIKELFEVQDIG"
     gene            complement(23508..23951)
                     /gene="rpiB"
                     /locus_tag="PK252_10225"
     CDS             complement(23508..23951)
                     /gene="rpiB"
                     /locus_tag="PK252_10225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT15124.1"
                     /translation="MSKIGIASDHAGYEMKEQVKAWLAEKGHTMVDFGCNSAQSADYA
                     DYAHPLANAVENKEVDFGVSFCGSGNGITMTLNKHQGIRAALCWNEEISALSRAHNDA
                     NVCSMPARFITFDTAKKIVEAFINTPFEGGRHKTRIDKMPVTGCK"
     gene            24113..24487
                     /locus_tag="PK252_10230"
     CDS             24113..24487
                     /locus_tag="PK252_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15125.1"
                     /translation="MSERCSFININGKNVYFMDFSNLNSRDEIFKYIAEAKQVIRSQA
                     PSSVYALTNIEKMHYDSEIKEAFTEFTKGNKPYIKYSAVVGIDTLQRILFNAIMKLSG
                     RDIRVFANLDEAKFWLIDKTLS"
     gene            complement(24598..25272)
                     /locus_tag="PK252_10235"
     CDS             complement(24598..25272)
                     /locus_tag="PK252_10235"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF013394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT15126.1"
                     /translation="METFKGLMKGLITTAIFTIILFLSAGRLDYYQGWVFLAANTLTT
                     VVNVVTLRKNTDLLIERSKLGEGIKSWDKWLLALSAMVYLIMIIIAGLDSGRFLWTKS
                     FNWFISAIGVAILAIGQSIFLTARNQNNFFSSMVRIQKERGHTVCDKGLYKIIRHPGY
                     LGMAVSLFAVPIITTSFWCILPTTIAVILLLIRTSLEDKTLKAELDGYAEYSKHTRYK
                     LIPLIW"
     gene            complement(25501..>26434)
                     /locus_tag="PK252_10240"
     CDS             complement(25501..>26434)
                     /locus_tag="PK252_10240"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOT15127.1"
                     /translation="KGEKKKIERVLDFACGSGSLLLNVRKKMTGAGGTIGKIFGQEKN
                     ITTYNLARMNMLLHGVKDTEFEIHHGDTLLNDWDILNEMNPARKLEFDAVVANPPFSY
                     RWEPTEAMGEDFRFKGYGLAPKSAADFSFLLHGFHFLSKEGTMAIILPHGVLFRGGSE
                     ERIRRKLLEDGNIDTVIGLPANLFFSTGIPVCILVLKKCKKFDDVLFINAVDHFEKGK
                     RQNTLLPEHIDKIVNTYKGRKEETRYSRRVSMDEIGKNEFNLNISRYVSTSVDEEIID
                     LKEVNKKLAELDKEINKAREEHNKYLNELGLPPI"
BASE COUNT         7888 a         5218 c         4886 g         8342 t
ORIGIN      
        1 aaaataaatt aaaaatcaaa ttattgtgtt tacacaaaat attttatact ctcggtaccg
       61 acgttttcat aaaaaccatg aaacttgtta ttttttttca ggagtagcag cttctttttt
      121 ttcagctttt ttttcagctt tcttatcgtc tttaccctct ttcttggtct tagaacattc
      181 tttttttgcc ggttcagact ttgtttgggt tgtttgtgct gatactactg ccaaacttgc
      241 tactaacatc atagcgactg ataacaatgc aatttttttc ataactttta tttttaaaga
      301 ttaataacga ttcaaaagta cagctgctat aagtgctaaa acttttattt taacaaaaga
      361 tatgttaaaa gaagtgttaa gaagcgttaa tttaaattca aactaacttc attccgacat
      421 aagaaaaatg cttaggtatt tgatatttgc aggcattgca aactcttaaa gcaacgaatg
      481 gataaaattt ggatgatcca tctgataatg cacaatttaa aattttactc tcataaaaac
      541 acccataatt gctcaatcgc aaacacgaaa atagttattc ttatatcaga ttcgggctat
      601 tttgcaactt gacagaagtg aataatcatt tttggatgtt atcaagaaag tatgttatgt
      661 atggaaatct ttgtttattc taccacaaac agcttattgc ggattgcata gataacaagg
      721 ctggccgtat tgttgcagtt ggtctttttg aggagatttg cacggtgcga ttttacagtg
      781 gcaacactga tattgagttt atttgctatt tcatcattag atagccctgc tgcaataagt
      841 tttaaaactt caacttcacg attactcaac aagtctttat ctttagccga ttcaattttt
      901 gctttgtcag tttttatgtt ggtaattatt tgcttaacca gttcggccga gaagtaaatt
      961 tctcctttgg ctacttctgt aatggcattt tccagctcgg ctatgcccga tgatttaagc
     1021 acaaaacctt ttacgccagc ctgtatcatt ttaaaatagt attcttcgtc gccaaacatc
     1081 gaaagggtaa gtattttgct gtcggggtca atggccattc tttttcgtgt agcttcaatg
     1141 ccatccataa caggcatatt aatatccatc agggtaacat caggcgaata tttatctact
     1201 ttatcaaggt actcctggcc attttctgct tcgccaacaa tttctattac tttacttttg
     1261 tttagtaaaa ccttaactcc ttcgcggaac atatcatggt cgtcaacaat aaatactttg
     1321 agttttttca tttgtgtaaa tatttttttt aattaagcct ggatgggccc cgaagggttc
     1381 tccgaaggaa aactcgcatg atgaattttc atccttgttt gtgtttaacg aatcatgctc
     1441 gtttcattgt catagctttt agcttatttt aatggttgct tttattttaa tacctttgcc
     1501 tgtttctgtt tttatttcac tatttcctcc taaggattgt atcctgttct ggatattgta
     1561 caagcccatg cccatgctgt tttttttcac tgtatcgtaa tcaaaaccaa caccattatc
     1621 agcataatca aaataaagca aatcgttttt taagtataaa gatataaaac tctctttagc
     1681 tttagaatgt ttttgggtgt tatttataca ttctttgata acccaaaaca aggccgattc
     1741 gcaattgtgg ggcaaccttt cgcataggtt tgattggaaa cggaaattta tttctccttt
     1801 ttcgttcatt gtatctatta atgattgtac tgcagcgttt agcccaaatt tttcgagcaa
     1861 atgcgggctc aggttgtacg agatactgcg cagctgggtt attgcttctt ttattgtttg
     1921 ccttgaaagc cgcagtattt cttcgctgtc ttctttacgt gttttatcgg caagccattc
     1981 tatgtacatg ttgagggttg aaagtgtagg gcccagccca tcgtgcaagt cggtggcaag
     2041 ccgcttgcgc tcgcggtctt cggcttgtac tacttcgttt aatattcgct gggccatcag
     2101 tttacgttcg gtaatatcac gcaaaatgct aagtacaacc ttttcgccat aatagttgat
     2161 ggtagtgctg tttgcttcaa taaacctaat ttcaccattt ggggctggaa gctgatattc
     2221 gaatattggc gtggggttag taaatatttc ctgaaaacgt tgttcgaaaa tttctaatag
     2281 tttgtccgaa agtaaatcgg tgatttttaa ccgtgtacct tttttggcct gtacaaaatt
     2341 gtaaaataca gggttggcca tcaatatttc tttattggca ttgtatatga taatagcgtc
     2401 attgctggcc tcaaatatct ttaaaaactg tgtttctctt tcttttagct gtgtcagggc
     2461 tttttcgaga agggtatttt tgtctttcaa ctcattaaaa agccttttat tttcaatgct
     2521 caggttgtac gaaaaaatag cactgtttat gcttgcgcgc atttcgttgg tgtcccaggg
     2581 ctttaaaaga tagcggtaag tgttcccttt gtttatcgcg tttaaagcag catcgaggtc
     2641 gtaatgcgaa gtcaggataa tataaaacac atcagggtac gactttgaaa cgtcttcaat
     2701 aaagtccgta ccgctttcct gcggcataaa taagtcaaca atggccactt ttatttcatt
     2761 gttgcccatc atttgacgag cttcggtagt atccgaagtg gttaatacct ggtaatcgtt
     2821 ttcaaaaata gctgtaaaca atgtcaggtt tgtttcgtca tcatctatat acagtacaat
     2881 ttctttttcc aagctgtcaa tatttatttt tgaaattaat gaaaaaaatc aggttttcaa
     2941 aggcaaaatt aaatagcagt aaactttgtt taaaattcat tgagttgata gttttttgac
     3001 cttaaaaatg cattttattc aggtccactt tttttatata tatttacgcg acaaaaattt
     3061 acattatcat ggaacgtaac cgtagtatta ttatcaggtt tttatttgcg ctgataagta
     3121 tttcttattc gttttttttt atatttaaat ttatccgacc cgaattatat tttcattttc
     3181 aacaacctcc atttttattg acctctggct ttttttacgg ctatttgtct tatcccggcg
     3241 gattttctta ttatttgtca gattttttat cgcatctttt ttatttcaat ttcttagggg
     3301 ggctgatcat ttttgtttta ggcctgctta tttatttttt attttattgg actttacggc
     3361 ttaaggcaaa atttttcatg gcaactattc ctgtggccat gttttttgct ttgttatgca
     3421 atgtttattt cccattctca acaataatta gttttatcat cacattgtcg ctgattcttt
     3481 taacacgcaa tttttcaaaa aatacttctt ttgcctttat actaatcatt gtaggctttt
     3541 tgactactta ttatctggct ggtataggat atacctatgt ttatgccatt tcatctattt
     3601 tggccttact ttcaaacaaa gccttaaaga agagattttt ggcaggggct gttttggttt
     3661 ttattatatc ggctttgttg tatctgctgg ccaattacat gttcttgctt tccccatctg
     3721 aaatgtattg gacttttttc agaaagctgg ctctatttat gtcttataaa ccaacaaaat
     3781 tttactattt gtttgttttt tctccattaa tattgatggc tgttgatatt ttggcagaat
     3841 ggaaaaaatc ccattggaca ttggaatatt tagataaagt aaagataatt gctgtagcat
     3901 cagtattttt tgctattttc atttcaattt ctctttattt tccttataaa tgggacttaa
     3961 gcaaaaaaag aaattttgta ctttgcgatt attataacga acagggcaaa tgggaaaaaa
     4021 ccatagaagt ggcaaaacaa agcacagagt atgattattt gacaaattta aattttagca
     4081 gggctatttt caactcaggc aattcattaa accaactgtt tgactatccg caggttgttg
     4141 gtaaagatgg tatccatcca gattattcct attcgggcga aatagttttt ttgtcgcaag
     4201 atgcatatta tgacctgggg ttaataagcg aatccagaca ttgggcttac gaatctttta
     4261 ttgggtttcc ttataacaca agggctttaa aggctcttgt aaaaatacat tttattaacc
     4321 gccaatttga tgctgctgcc aattgtcttg atattttaga aaaagggctt attagcagtg
     4381 attttgtaaa aaaatacagg ccttatttaa aagataccaa ccttgttaac aatgaccctg
     4441 aattagcaga gaaacgaaaa aatatgccaa ccggctttga aatttctgaa tctttagaat
     4501 tgaagttgaa tatcttgctt gaaaaagatt caagcaacaa aaaagcacta gaatatctct
     4561 tggcttttta tatgttagac tctcaactgg ataaattcat gtctttagta gattatgctt
     4621 ctcaatatta taatcaatgg cctgaaattg tccaagaggc tattgttgtt tatggtgctg
     4681 taagaggcaa aaaagtcatt aaagagtatg gcatcagccc aaatactgtt gaacgcttta
     4741 aatatttcag cctcaccttg cgaaactgtg gcaaagacat ggatttagcc aaggatttgt
     4801 tatatccaga ttttaaaaac tcctattttt actttttcaa atttcttaac cctaaagtga
     4861 caaacgccaa gattgtagta aaccttgata ataactcatc aatttaaagt aaaatgaaca
     4921 agtatatttg ggccatctat attgtattac ttcttgctgt aagttgtaaa cctgatgttg
     4981 ctgattttat agacgagaaa aaagaagctt caatatttcc agactactct ggccttgttt
     5041 tgcctgctaa tatagcccct cttaattttg ttgttaaaga aaaagcagac aaatacttca
     5101 tccggattaa atcaagccgg ggaaacactt atgatatatg ccaaacttcc cccaaaattg
     5161 acatttctga aagaaaatgg aaaaaattac tttcggaatc agttaatgcg aacctttaca
     5221 tctcaatatt tgcaaaaaaa gatggtaaat ggtataagta taatgatata aaggacagcg
     5281 tagttgacaa aaaaattgat aattacttag tatatcgcgt tgtatatgcc aactatctat
     5341 tttggcgaaa aatggaagtt attcaaagag atttagagtc gtttaatgaa tatactgtgt
     5401 atgacaatgc ttcgggcgac gatggatgtg ttaactgcca ttcttttccc aaaaacaacc
     5461 caaacaaaat gatgatgcac tttagggtta tccatcccgg tatgttgcta aaccttaatg
     5521 gccaaataaa aaaaattgat acccgtaccc aatatacctt gtctggtgct gtttatccag
     5581 cctggcaccc ggatggaaag ctcattgctt tttctacaat taaactatcg ccccatatta
     5641 caaccgatat ccataagatt attgatgcct ctgataaagc ttcagatttg attgtttata
     5701 ataccgaaac aaacattatt accacttctc caaaaatttc taccaaaagc agggagaata
     5761 tgcctgcctg gtcccctgat ggtaaatatc tttatttcat tgttgcccct caagttgaaa
     5821 atcaggatat taagtctttg agaaaagtaa aatatagcat tgcgcgcatt gaatacaatg
     5881 taaatactaa tacatgggga aatgtggaca ccgtattatc tgcttcacaa actggaatca
     5941 gtatgtcttt cccactaatt tcgcccgatg ggagatattt gctttttacg gggactgatt
     6001 atggctattt taccgctttt cacaatcagg ctgacttgta ttgctatgac cttcaggcaa
     6061 agacactttc aaaacctgct ctgaacagtt caagttgcga aagctatcat acctggtcaa
     6121 gtaatggtcg ctggattgtt tttagcagca aacgtatgga tgatgtttat accaggcctt
     6181 ttatagcatt ttttgatgaa aaaggcaata caagtaagcc ttttgttatg ccaataaaag
     6241 accccgatga atattcaacc cttctggcca attacaaccg gccagagctt gtaaccggca
     6301 aaatagagct tagtaaccgg caaatcagag atgctgtttt cgctgaacct gttcctgcaa
     6361 aatttgatac ctctgttgag gtagatgctt tgtcgggggc tacagccaaa aagtgagaaa
     6421 tatatgggac tatgtcatca cattcttaaa ttttacagaa aataacgcat atcaaattta
     6481 gcgcttcagc aagacgttat atttgttata ctcttgttgt ggcgcgcaac ataaatgtaa
     6541 ataataactc ttcgggtgtg tggtattaaa gctaatgaaa cacccatgtt cttgcgaacg
     6601 caactaagcg aagcgccctc atgaacgaag tgaataaaca cgatagtaaa gtacaatatg
     6661 ggtgttcgcc cgccaaagac gggacaagct atcattcctt aaaaatttat tctcatgaaa
     6721 ataccaaaag aaaacgctta tttcacctca actacctgcc cggtagaata aatgccctgc
     6781 ctttggatac tgaggttttg tggcattttt ttacaaaaaa cattgcccga tgagcaaaac
     6841 aatactgaca actgctcagg ttcgttgaga tatacctctg aaatgctatt ttgccttaca
     6901 tagcacgatt gcatttgaag gttttcggcc ctaaaaagcg ttgacccgct ggcataaaat
     6961 gcagcatggt cacatttccc attgatcaca aaattgccaa agctgtcata aggggcaaaa
     7021 atggcacatt ctgatacatt tacagtaaca tttatttcgc aaaacgattt aaaatccata
     7081 taaaaacggg ctgaacagaa agtatcgatg gtagaaaccc tcacaggggc tgcaaaataa
     7141 aacttaggag aggaagaaac atgtatttcg agctccatag ggtcatccag tttataccaa
     7201 tggcctccta tatggtggga gataaaaagt gaatcgtggt aaagattaac agacacctct
     7261 tgtatcagtt tttcgcctcc cttaaaaacc agataatttg ccgaatcact tatcagtatg
     7321 atatggaaag ctttgcccac ttcaacgtat gtaaaattgc tttctaaaag tacttttttg
     7381 cttttttcgg gaccagcatc aaaaaacatt tcctttttgc acgaacagca aatagccaaa
     7441 accaaaagaa ctaaaattct gaaatacttc acaacacaat gttttaattt ttaaacctgt
     7501 aaccaatgcc ccactccaca acatcagctt ttgccgccca atgcgcattg agcgtaagtg
     7561 cagccacaag cccccaattg gtataatacc gcataccaag acggttacaa atgttattgg
     7621 ccacatcaat cttatttttt aaatatatcc ccggctgaaa aacaaaagaa aggttgccca
     7681 ttcgtatctc gtacgaagcg ttgatggcca cacgcaattg gtcattgccc gtcacaaccg
     7741 aatcggcttt gacaaggtgt attttttcaa caatagaagg atcgtaagat aagtaaaggt
     7801 tgagccctac atatcggcta atatagagtt ttctgttgta agccaggtta accgaataaa
     7861 gcggaaaaat tttgttgtca aacctcgaaa tatttttcct tccaatggat gtaagcacaa
     7921 taaactcatt ctttgccgaa tcggtatcta caaatgtttg cctagggcgt ttgggcattt
     7981 ctctaaaatg atattgtacg cttgcagagg tagtaaaata attcatgccg gcattgggct
     8041 ttctgaaaga accattggaa gtatgcgaaa atccggcacc agccagtatc ctgacttgtg
     8101 aagttgccca atacgatgcc tcaaaacaaa gacgggcata aacattgtaa cgcgaaccta
     8161 aggccaaatt ataagggttt gtgtactggt cgtaaaaacg cgtggcatac gccaatccca
     8221 agtcgagccg gttgcctata ttgaaacggc cgtttacatc aaaatagtaa cggtctacaa
     8281 agaaatacag cgagtgcaaa tgtccgtata tctggtcatt gcccagcgaa ccctgatagt
     8341 agcccattcc aaatgaagga taattaaaaa cctggtgcca cgccttattg cccgaaacca
     8401 ccttgccaaa agctacctgg aacgattgca caggctcgtg tataaaaaag gcaaggtaat
     8461 ccttatggga cagcaaatag ccgtactggt aagagccttc catgaaaaag tctttttcta
     8521 aggccgaaac ctgtcctata cccttttggg ctaaaatcaa aaacaacgtc aataatatat
     8581 atttggggtg tttcatcatt aaaaaaccaa gctgcaaaat tatattaaaa aatcaagctg
     8641 caaaattata ctaatatatt caacttgcgt ttgtcaggcg atttttttta taaaactata
     8701 cctttgtact ccttacttga aatgctttag ccatggacaa cacattcaat aacgacaaaa
     8761 aacagcttat caccgccatt ccatacaaag taaccactgc aaatgccgat atgcacctgc
     8821 gtttgcgttg gggggctttg acagaccttt tgatacaatc ggccattgca tcggcagaca
     8881 ggttacaagc cggatttggt cagcttaagc agcaaaagct attttgggtg ctcagccgca
     8941 tgaccataga aatttcgaac ccccttactt tgggccaaac aggagaagta gaaacctggc
     9001 ctaaagatgt cgaaaaaatc ctctatctca gggattttgt ggtcaaaacc cacgagcagc
     9061 aaatagttgc caaagctacc tctggctggc tggctgtgga catggaaaac aggcgccccc
     9121 gaacgctgga aggcgactat tctgctattt tcagtaaact gaaagacttt catgccctgc
     9181 aatctcagcc cgaaaagctt ttcgcggtga aggaaggcga agcatctgaa gtaaaagcca
     9241 catactttga tatcgacctc aatggccatg ttactgcaag ccgctatatc gactggatga
     9301 tggatacctt cccggctgac tttcaccgca accattaccc caaaacgctt tccatcaact
     9361 acctcaacga aacccttatc aacgaaacca tccggcttac caaagctacc aacgacaata
     9421 aaaactttct gttcgaaggc ttgaacaaag acagaaacat cacggctttc aggggaaaaa
     9481 ttggcttttg aaatagtttt tatgaattag gcccttaact aatagtgctg gtctatctta
     9541 ttttaccaca gagaacgcta atttttttta ccacagagaa ctgcaaagtt gaaataccta
     9601 ctttgcggtt cttcgcgtta atctttgtgt cctcagcggt tttaaatatt gtttatgagc
     9661 ctaaaacaac aaaaatgatg aaaataaaat tctagatatt tgttatgaca ttgttgtatg
     9721 caaggctgaa acccgtccga actataggta aagcttgttt gaaaagagaa aaaacagatt
     9781 tgataaaccc accgctcgtt gcagtacatt tgtaaaccaa aagcttgaaa aagagctaca
     9841 atcttgccta tgcaccgtct tgtagataac tgattacctt gtttacacat ccatcaaaat
     9901 ctttttttaa ttcgttttcc caaaaacgga ttacatgcca accattatct ttgagaagtt
     9961 gattattctg tttgtccctt tggatattac gttcaatttt tgggatccaa aattcccggt
    10021 ttgttttaat tttcgttttc ttttctgacc agttataacc atgccaaaat tcaccatcga
    10081 caaatattgc caatttgtgc ttagaatata caatatcagg actgccagga agtttcttaa
    10141 aatttttcct gtatctgtac ccaaggttcc atagcgcttt tttgagttta agttctggct
    10201 ttgtattttg ggattttatt ttgcccataa gttcagacct ttgcttggtc gtataaaaac
    10261 cattaacttc gttaaacctt ggcacaacaa tatgattttc agtatatttc ttcaatgcaa
    10321 aaaatgttaa taattatctg caaattaaat aatagtaggt taaaattaaa attatatttg
    10381 taaccatgta taatgttgat taatgtggtt ataaaatgga aaaatattac tcaatagcag
    10441 aggttgcgga tttattgtcg gttagcaaag aaaccttacg gcgttgggat agaaatggaa
    10501 agcttacagc aatccgtgaa ccgatgagta attacagggt ttatcacaaa gaccaattaa
    10561 aaatatttga cagccttgac tttttattta tgcctaccga aagcgtaaat gaaacaaaac
    10621 caacccgtga tttttactca attgaattat ttgcaggagc cggaggattg gcgattggat
    10681 tggaagaggc aggaataaaa tgtttagcac ttaatgagtt tgaccattgg gcttgtgaaa
    10741 ccttaagaaa aaaccgtcct gactggaacg ttattgaaga cgatgttagg aatgtgtcat
    10801 tttcaaattt taaaggtaaa gttgatattg taacgggtgg atttccttgt caagctttta
    10861 gttatgccgg taaaaaactc gggcttaatg atgcaagggg aacattgttt tatgaatttg
    10921 caagagttgt tcaagaaacg atgcctgcaa tttgcattgg agaaaatgtc agggggttat
    10981 tgacccatga aaatggcagg acaattgaag gtatgatttc tattcttgat gaaataggtt
    11041 ataatgttat tacaccaagg atttttaaaa caatttttta taaagtcccg caaaaaaggg
    11101 aaagggtctt gattgtaggg attagaaaag acttaaaccc acacctttat gaatttccta
    11161 aaccatttaa aaaagtttat acacttaaag atgctttaaa gaaaggggaa ttatataatt
    11221 gtgatgtgcc agagtctgca ggttgtaaat accccaaaag taaaaaagag gttttagatt
    11281 tagtgccgga aggtggttat tggcgggatt taccaattga tgtacagaaa aaatatatgg
    11341 gcgggagttt ttatttagga gggggaaaaa caggtatggc aaggcgtatg agttggtctg
    11401 aacccagttt gactttgaca tgcagccctg cccaaaaaca aaccgaacgt tgccatcctg
    11461 atgaaaccag gccatttact gttagggaat atgcaaggat ccaaacattt cctgatgatt
    11521 ggagtttttc cggctcaata tcccagcaat acaaacaaat tggcaacgct gtacctgtaa
    11581 actttgcaaa agagattggc tattcaatta taaggttttt aaataaaatt tatccaaagc
    11641 agtattaagc ttattgcttg tggtacaaaa tatttatttt tatggcttaa aatattaaag
    11701 ttaatgccat gccttatctc aaatggattt ctgatactga ccttgaaaaa gaagtggatc
    11761 aattactttc tattgcaaaa gatgcaaaaa aggctgcaat tgaagaattt ggtaaaaatg
    11821 taattgaccc atttgcggcc cttttcgaaa tggctggatt tgagattgat tacgatactt
    11881 ggttaaaaag tgaaactacc agacaggctc aaaaaacttt gcagaaccat ataggtaatt
    11941 ttcaccaaaa cattcttggg tattgtaagg gctggagcaa taaaggtgtt ggcaatataa
    12001 ttgacttggt ttctttggac aacaaaatta ttgcggaaat aaaaaataag tacaatacaa
    12061 tttcaggtgg gaaattatct gatttatatt attcattaga aaaacaagtt agccccaagt
    12121 ctagtattta caaaggttat actgcttatt atgttgcaat aatccctaaa aaaggagaaa
    12181 ggtttaataa accatttact ccttcagaca aagacaaagg ggagaaatgt cctataaatg
    12241 agcatatccg tgaaattgat ggggcaagtt tttatagttt agtaactggt tcggaaaatg
    12301 ctttagagga tttatttgat attttgccgg aagtaataaa gctttgctca gaaggcaaat
    12361 atcaaataaa agacaagaaa aaactcaaag aattctttaa tttggctttc aaatagcctt
    12421 atcgcaaggt caagctcttt tgcaatttta agtcggtgtg gccgctttgt gcgaattgca
    12481 aaagagcttg aaaccaaaag aggtaaacaa ccaatatttg tcaacgccct aaaaatatgg
    12541 tatttacgca tgggtattga taatcaggat agattagact taacttctca gctattggcc
    12601 tggcgtaaat ggtgttaaga aaaaaacttt atcggcgaag cgaaggccac tgtttggaca
    12661 aagcgggttg ttgcccggag ccgtcgataa agcttttttt agccaattta cggcaagcct
    12721 tgaatttttg ttacttttgc ttcaaggcaa aaggaagcaa tgttcagtta aagcctgtaa
    12781 atatcagaca agtaagcatt cttcttatgt cgaacagacg ttaaattatc tttataaaca
    12841 aagcaaactg aacaaggatg aaaatactga ttatccaaac tgcttttatt ggcgatgtag
    12901 tacttgctac ccctgttgtt gagaaactca gggaaaaata ccccgatagt accattgact
    12961 tcttattgcg aaaggggaac gaatcgttgc tcattggaaa ccctcatatt cgcaaggttt
    13021 gggtttttga caaaaaaaac ggcaaatata aaaacctgtt aaaaatggcc aaagagctga
    13081 gaacagaggc ttacgattat gttatcaacc tgcaacgctt ttttactaca ggtttgatga
    13141 cagtactatc cggaggcaaa gtaaagatag gctttgacaa gaacccgctc tcctttttgt
    13201 ttacacatgt tgtaaagcat catatatcac atgacgacat caatgttcac gaagtggaca
    13261 gaaacctttc gctcatcgag ccaatagttg gtgatactgc gttcaaaaag ccacggctat
    13321 acccatcttc aactgattat gccgttgtcc ctacgggtga agaatacatt tgcattgccc
    13381 cttcatcggt gtggtttacc aagcagtttg ccgaacataa atggatagaa ctgataaata
    13441 atatccccgc aaaatataag gtttacctca ttggggccaa aagtgacatg cctctttgcg
    13501 ggcgcataca aaaagctacc agccatcagt cagttgaaat cctggctggc cagttgactt
    13561 ttcttcaatc atgcgccctt attgacaaag cccgtatgac atttgtgaat gattcagccc
    13621 cgctgcattt tgcatcggcc atgaatgccc cggtaacagc tttcttttgt tcaacagtgc
    13681 cagcttttgg atttggccct ttgtcagatt tttcgattgt tgcagaagtt gaagacaagc
    13741 ttgattgcag gccttgcggt ttgcatggca aaaaacaatg cccgcagcag cattttaaat
    13801 gttcggaaat ggatgtgaag aaggttttgg caaagaccaa aattttataa acccattttt
    13861 tttaaacttt tcgtaacaaa aaaaatgtgg caaatgtatt tgaattactc ttgttttttc
    13921 gaaagcaccc tttttatcat gtccaacaat atttctttac ggataggttt tgagatgtaa
    13981 tcgtcgaagc cgacagccct gatattttgt tcttcttcgt gccgggcaaa agccgtttgt
    14041 gctattacag gcagctcggg cttttcagcc cttatgattt taaaagcttc gatgccatcc
    14101 atgtcaggca tttttatgtc catcaaaacc aggtcatact tgttttttcg gcacatatcc
    14161 acagcttcaa tgccattttt tgcccagtgg acaattgctc cctgttggcc aagcagttta
    14221 cttaaatata aataattgcc tttttcgtct tcagccaata aaataacttt gtccgtaaaa
    14281 acggggttaa tgacagagat agtttgcatc tccttttgat cagctgtaaa aggtttttgt
    14341 accgacaatt gagaggaaag ctttacataa aatgtagaac cttcacctaa ttttgattgc
    14401 acttcaaggc ttccgccaag cagctccatc aggctttttg agatagcaag cccaaggcct
    14461 gcgcccctga atactttttt gttatcatcg tgccctttta caaagcggtc aaatataatg
    14521 gcctgagatg cctcatcaat tccaataccg gtgtctttaa cagcaaaaat atactcgcca
    14581 ttgctttctt ctacctttag ttcaatactt ccggcagggg taaatttaaa ggcattgtcg
    14641 atgaggttga gcaaaacctg cctcagcctg tgcctgtcgg tttcgatgag cttttctcca
    14701 ctaatgttga gatttaggta aaaatctact gattctttgg gttttttaat ctttctgtaa
    14761 ctcagtatca aatcatggat aaaagttgaa acattgaaca cttctttttt gatttcaatc
    14821 tggttggctt caattctcga aaggtcaaga atatcgtcta tcaaaaccag caaagcttcg
    14881 ctattggcgt taatcaacct gataaagtct tctttttctt tttcggtaat atcaggggca
    14941 tttagtaatg acgaaaaacc aatgatggca ttcatcgggg tcctgatttc gtgcgacata
    15001 ttagacaaaa aggccatctt cagcctgtct gattcttctg ccttttgttt tgccacttcc
    15061 aactcggctg tccgttgcgc aacaagctct tcaagatggt tgcggtgctt ttcgagttca
    15121 atgttttgct gttgtatttg gttttgtttt tcttccaggg ctttatttac catttcgagg
    15181 ttttcttttt gtgccaatac ctcagcccgc tgataatcaa tctcttggtt ctggatttct
    15241 aacatctcgt tgagctcgac caattctttg gtacgagtgt ccaccattat tttcaggctt
    15301 ctttgctgct tttcaaattt ggtaagcctt tctttaacaa caaaataagt taacacaaca
    15361 aaccccgtga taagaattac ccaaaacgtc caggttttat aaaaaggagg cctgataacc
    15421 aacaaaaccg aagcgccgtc ttcgttccaa acattatcgt tattcgaacc ttttaccctg
    15481 aatacatatt tcccggggtt caggttggta taagtggctg tacgctggtt gccaacgtcg
    15541 ttccagtttt gttcaaaacc ttcaagctta tattggtacc tgttttttga aggcatcaca
    15601 taatctaaag cagcaaattc aaaggttata accgattgtg aatagtctat ctcaataatt
    15661 ttgcttttta taattgaaac tttcagtggc gaacccggct cgtcaactgc tacagactta
    15721 ttgaatatgc gaaaatcagt tagtacaaca ggaggtacat tagtgtttat tttcaggctt
    15781 tcgggcataa aataattgat gccattgata cctccaaaat ataacaaacc cgatctggcc
    15841 atcatggccg attgtggcga aaactcattg cttagcaggc cgtcattcag gtcataattt
    15901 tgaaaaaaag ggtattgtat gttgttttca gaatatttaa gttttgaaag gccgttgttt
    15961 gtgctaatcc agatatttcc ttctttatct tcaagaaatg cgtaaactaa gttactggca
    16021 agtccatctt taacggttaa cttataaaat ttctcaacgt cttcatcgta cacgttaatg
    16081 cccccgccca gggttcctac ccaaatcctg ccttttgaat cttcaaatat ggaacatatc
    16141 tggttactgg taagtgcact gttattatta tctgagcgga caaaattctg aaacttgccc
    16201 cactcgcctt ttttcattac cgaaatgccg ccaaattcgg ttccaaccca aagattgcca
    16261 tttttatcaa actcgatgac cgaaacacca taatctgaaa gactggaaga atcttttgga
    16321 ttgtgcaaaa aatgggtaaa cttcctggtt ttagggtcaa aacaatcgag gccctgattg
    16381 agtgtagcaa tccaaatcat gccatcctca tcctggtcta tgtcccatac atggtttgag
    16441 gcagggctgt tgggattggc cgggtcgtgg taataacggg taaatttcct ggttttaggg
    16501 ttataggcat tgagcccttc gttgaaagta ccaatccaaa ggatgctgtc tttatcaaac
    16561 attaatgatt ttacaacagt accactgagt gaattgggat tattggggtt atttctgaaa
    16621 tgctcaaact tgttttcacg gggattatat ttgtccattc ctcctccatc agtcccaatc
    16681 caaatgtttc cgcttttatc ttcaagcatg gtaaggacag agttggccga aaggctgttc
    16741 tcttcaccaa aactacggat atggccaaac ttgtctttta cttcaaaaac aacatccacc
    16801 ccggcattgt acgtccctat ccataaattg tgcatttcgt ccatcaaaaa gcagtaaatg
    16861 acatcagaac ccaggctaaa ctggttgttc agctgatttt taatactttg gcacacatgc
    16921 ttgttttcat ccagaatatt taagccactt ccgcctgttc caattattac atttttgttt
    16981 ttgtcttcag taaaagccct gactatgtta tgcccaatgc ggtatggccc cggggaggag
    17041 gtagtgtatt gcaacatctt tttgtttgag ggggtatatg caaaagctcc ttttccgtat
    17101 gtcccaaacc acatggtccc atcagaagcc ctgtatatac ttataatatt aatgtttttc
    17161 aatagcccaa aaggctccga tttaatgttg gcgtaataac catttttggc atttctgatt
    17221 atctcaacgc ctgaggttgt gcctatatac aggttgctgt cattgtcata acaaatggcc
    17281 gtaacattgt ttttgttgct caatggtacc gacctgaaag atttatcttt agggtcaaac
    17341 aagctaatac tcccttcata acaacccagc cacaggcggt tatcggcagt ctctaaaata
    17401 cccctgatat cgttggtggc agcagcaaca acatgctcaa tgccactttt aaaatgaaca
    17461 aatcgttcgg tattttcatc gtaaaggtca aggcctccct ggtctgtacc cacccacatt
    17521 tggcccgatt tgctgatata aatgcagtgt acccggccta acccaatggt ggtgtaatcg
    17581 ttttcgtcgt gcttaaaatg ctggacagta taaccgttgt aacggtttag gccgtcatct
    17641 gtgccaaacc agataaaacc agtcctgtct ttaacaattg tattgatggt actttgcgaa
    17701 agccccgaat tgcgggtaat atgataaaat tttttgaaag tcttttgggc atgaaggctg
    17761 ttaacaaaca agacaatgac aaataattgg atacaatatt gtagtttaaa tttcatcagt
    17821 tttgttttgc tttacttttt tattgaataa aaattatata tcaacaaacc tcctaaagaa
    17881 ttgaattaaa gataataaaa aagctcaaat tttgtatatt cctaatatca aagtttattt
    17941 tttgcacaac tacacgtttg actttttttt aaacttaaaa aaataactta tttcattatt
    18001 aatcaatttt ttacaacgaa atattttttt atttttttaa ttactattat ctcaattgct
    18061 atataacttg caggaaaaat tactttctgg taacaaacta aattctattc tgatgaaaaa
    18121 aatattcctt ttgcttattg cttatgcctg gacacagctg aatgcagccg agctgaaatc
    18181 gcctaatggt cagttatcat tgaatgtaac agtgatagat agtgtggcta cttaccgtct
    18241 tgattataaa agccgtccgg taattaaaca aagccgcctg ggccttgaac taaaaaaaga
    18301 taaagacctt attcacggtt ttgtaatttc taaggttgat acctctactt ttgacgaaac
    18361 ctggcagcct gtatggggcg aagtaaaaaa catccgcaac tattacaaag aattggccgt
    18421 tacattgtct caaccaagta caaagcgcag tataatagtt cgtttcaggt tgtttaatga
    18481 cggattgggc ttcagatatg aatttccaca acaagataac ctcaattact ttattatcaa
    18541 agaagaaagg acacagtttg ctatgacagg cgaccacaag gcattttggc ttcctggcga
    18601 ttttgatacc caggaatata gcacggttac ctctaaactg tctgaaattc gcagcctgat
    18661 gaaaagcgct ataaccagta atgcctctca aacccagttt tctcctacag gagtacaaac
    18721 ttcactgatg ctaaaaagcg atgatggctt gtatatcaac attcacgaag ccgctttagt
    18781 tgattattcg tgtatgtcac tcaacctcga tgataaaaac ttcatttttg agtcgttcct
    18841 gacccccgat gccattggag acaaaggata tatgcagact ccatgcaaat cgccctggcg
    18901 gaccattata gtcagcgata aagctaccga gatactggag tcgaagctta tcctcaacct
    18961 caacgagcct tgccaataca gcgatgtatc atggataaaa cctgtcaaat acgtcggcgt
    19021 atggtgggaa atgattaccg gcaaaagcag ttgggcatat accgatgtaa atagcgtaaa
    19081 actcggcgaa tttgattaca acaaagccaa acctaacggt aaacatgctg ctaacacagc
    19141 tcatgtaaaa gaatatattg attttgcagc tttgcacggg ttcgatgcag tgcttgtcga
    19201 aggctggaac attggctggg aagactggtt tggcaaatgg aaagactatg tttttgattt
    19261 tgtaacgcct tatcctgatt ttgacataaa agaaatcagc caatatgcgg ccagcaaagg
    19321 ggtaaaaata ataatgcacc acgaaacctc aggcgcagtt cgtaattacg aacggcacct
    19381 cgatactgcc tataaattta tggttgccaa cggatacaac gctgtaaaaa gcggctacgt
    19441 gggtgatatc gtccctcgcg gcgaatatca ttacggccaa tggatggtca atcattactt
    19501 gtatgccatt aaaaaggctg ccgattacaa aatcatggtt aatgcccacg aggcagtacg
    19561 cccaactggc ttgtgccgga catatcccaa cctcattggt aacgaatcgg cccgtggcac
    19621 cgaatacgaa gcttttggag gcagcaaccc cgaccatacc actattctgc cttttaccag
    19681 gcttatggga ggccctatgg actatacccc gggtattttc gagacccata tttggaaact
    19741 caaccccgat aacaaatctt atgcccgaac caccctggca cgccagcttg cattgtatgt
    19801 taccatgtac agcccgctgc aaatggctgc tgacctgccc gagaattaca accgcttcct
    19861 cgatgctttt caattcatca aagatgttgc tgttgattgg gacgatacca gggttattga
    19921 agccgagcct ggcgactata ttactattgc ccgcaaagct aaaggaacaa acaactggtt
    19981 tgtaggcaat accagcgatg aaaatggcca tttatcgaag tttaaactca gcttcctcga
    20041 tgccggcaag aaatacaagg ctaccattta tgccgatgcc aaagatgccc attacgaaaa
    20101 aaacccacag gcatatacca tcaaaacaat agatgttacc aacaaaacag agctaagcct
    20161 tcaagctgca ccaggcggag gttttgccat tagcattgtt gaagtgcctg cgaaaaagta
    20221 aaaagtctga aaaggcttcg acttcgctca gcctgacgat tgataatcag caatttgtca
    20281 ttttgagcgg agtcgaaaaa tgaacaaatt tgaccttttt actaatcctt tttttttcaa
    20341 aaatcagtta aaaaaatgtt agcatgtaac cgaaaaaagc tccgctcaca ttattatgta
    20401 taactttaca taatagtcca taaccttcta aaaattttgt tatgaaaata ttttctgcaa
    20461 caatatttct cgcttttgta atgttcttta caagttttgg ccagacaaca acagctactg
    20521 acacttgcaa aaatgacgtg cctgaccaac gagggataat gaaaccatgg gaaaaagggg
    20581 cttatgaaac cggcaaatac cgtaacgtct ttttagaagc tggctataaa caagctgata
    20641 ttgatgcaaa acttgcaaaa gcctattacg atgtttttga aggccctaaa agagtgtatt
    20701 ttcaagtggg cgactcgatg gggtacgtct cagacgtaaa aaacaatgat gcccgcactg
    20761 aaggcctgtc ttacggaatg atggtagccg tccaactcaa taaaaaagat gttttcgacc
    20821 gcatttggcg ttggtctaaa aaatatgcac agcaccaggg tggccctcgt gatgcttatt
    20881 ttgcctggag cctagatacc gctaccggca acaaaaaatt tgccgggtct gcctctgatg
    20941 gagaactgta cttcgttaca gcccttttat ttgcctcaaa ccgctgggga aacaacacag
    21001 gcattaacta ttatgccgaa gcacgccgga tactcgatgc catgtggagc aaaaacggca
    21061 aagacggaat aacaaacctt atcaacgttg aacataaaca aataaccttt gtcccggata
    21121 cagcgggata caaatggacc gacccttcat atcatttacc tgcctttttt gaagtgtggg
    21181 ccatgtatgc caaagatgga cacgaacagt tttaccgcga ttgtgcagat actgccaggg
    21241 cattcttgca cagggcatgc catccggtta cagggctaaa tcccgactat gccaattttg
    21301 atggttcgcc taaacgggta tggaaggtaa tgggagatgc ttttcgttac gactcatgga
    21361 gagtacctat gaacattgcc atggattatt catggtttgc taaagacaag gaatggcaaa
    21421 aagactatgc tgtacgtttt cagaactttc ttttctgcaa aggcattgac acattcgaag
    21481 accagttcaa ccttgatggt tcgcgcccaa actggattct gggggccgga actgccagga
    21541 tccaaaaact tcgtcattca ctaggcctgg tatcaacatc tgctgctgcc tctatcatgg
    21601 gaacacaggc taaaagctgg aaatttgttg acgccctttg gaacgccaag ttggaaccct
    21661 atgaagacgg ctatttcgac ccttattacg atggcttgtt atacttgttt agccttatgc
    21721 acttaagcgg aaactatagg attattaccc cggtgggaac aaaataaaat cattgactct
    21781 attgcctgta attagtcaat gccgtttagt taagtaaaaa aatgccgaca ggtctgaaag
    21841 acgctgtcgg gttagtttca catctgacct gacagcgtct gcgaacgctg tcagagtttt
    21901 aactaaacga cagtgtaata ttagtttgca atcgattgag ggctacgttt attaactaaa
    21961 tatttatacc atttctccta catacatgag tagggaaagc gtcaggcagt caaattaagg
    22021 tttttgtgat aagaaaatta caaataagcc ctgtaagggc ttaaccttct tttttcttat
    22081 tgtaaaaatc aggttatcaa cgatggaggt atgtttataa acctcacttc atcaacccgg
    22141 caataatact gtccgacaca aaccatcctt ccaaattcat ggcaatcctt tcttcgttta
    22201 tgctttcaaa atattgcgaa taacggtttt ttatttcatt taatatatcg ttcgtaaaaa
    22261 ttggctgata tttttgcttc atctctgcaa tagaaatacc gtcactagtt cgcaacctgg
    22321 tcatcaggta ttcattaaac tggtcgcttt ccgaaaggtt ttcttcttcc cgacagattt
    22381 tattttcacg tattaaatca atatattgcc caatatcagc aatattccag cttctggcat
    22441 ggccattgaa tgaatgagct gcagggccaa gccctaaata cgaaacaccc tgccaatacg
    22501 agctgttgtg cttcgacttg tatccaggtt tcgacaagtt agaaatttcg taatgctcaa
    22561 agccattgtt catagatatt tcgtgaagca actggtattg tttccattgc atttcttcag
    22621 gtatctgttg tatcttgttt cgctggtacc agttataaaa aactgttcct tcttcataac
    22681 tcaggcaata agccgaaaga tgcaccatag gcagtttaaa ggcttccaat agtgtatttt
    22741 cccagtatat ctcatccgtt cccggtaaac catatatcaa atcaactgaa atattgttta
    22801 ttccggcatt ataaaattca tacaccgcct ttattgcatc ctccttgctg tgcctgcggt
    22861 tcatcagctt tagtaaccca ttgtcaaatg actggatgcc aatgcttacc ctgttaaaac
    22921 cgattgattt gaggccttta acgtattcgg cattgatgtc atccggattt acttcaatgg
    22981 tacattcaac aacctctgaa atatcaaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn ccaatatcct gtacttcaaa aagttcttta attttttcgc gaagcggccc
    23161 gtatttctca tgaatagggc agtgggcttc ggaagtagaa cagcttgtaa cacctattac
    23221 gcacgaagta aagctttcat ccccatcaat agcgtcaact atctccatca gcgatatttt
    23281 cttagctggc tttgccaaac aaaacccacc atttggacct ttggtagaaa gtaatacttt
    23341 acttttggcc aacgattgaa gaattttgcc caaaaatggc atgggaattt tcaaatcatc
    23401 ggatatcttt ttgatgccga tttttttttc ttcgtctgca ttcaaagcca gataaatgca
    23461 agccctgatg gcgtatttac aagtgtttga cagcatgggg atatttttta tttacagccc
    23521 gtaacaggca ttttgtcaat cctggtctta tgtcttcctc cttcaaacgg agtgtttata
    23581 aatgcttcta caattttttt tgctgtatca aacgtaataa aacgggcagg catagaacac
    23641 acattagcat cgttgtgtgc acgcgacaat gcagaaatct cttcgttcca gcacaatgct
    23701 gccctgattc cctggtgttt attcagcgtc atagtaatgc cgttgccgct tccacaaaac
    23761 gaaaccccaa aatctacctc tttgttttca acggcattag ccaaagggtg tgcataatca
    23821 gcataatcgg cactttgcgc actattacac ccaaaatcca ccatagtgtg gcctttttcg
    23881 gccaaccatg cttttacttg ttctttcatt tcataccctg catggtcact tgcaatacct
    23941 atcttactca tttttttgct tcaaaaataa ttaatatttc tatatctttt tttcttttat
    24001 ataaaactaa tccaaattgc tcatttattg taactttatg tttcaatttt taaaatattg
    24061 aagtattttt aataaaagtt gaattgttcc taacgtttaa aaacttaacc ccatgtcaga
    24121 aagatgctcc tttatcaaca taaacggtaa aaatgtttat ttcatggact tttctaattt
    24181 gaattcgaga gatgaaattt tcaaatatat tgcagaagca aaacaagtta tacgtagcca
    24241 ggctccatct tcggtatatg ctttgaccaa tatcgaaaaa atgcattatg acagcgaaat
    24301 caaagaggct tttactgaat ttaccaaggg aaataagcct tacatcaaat atagtgctgt
    24361 agttgggata gacaccctgc aacgtatcct tttcaatgcc attatgaaac tttccgggag
    24421 ggatatccgc gtatttgcca acctcgacga agctaaattc tggttgattg ataaaacgct
    24481 gagttaatca atactgattg ttgcaaaaaa ttttccattt caacttattg cgtgattttc
    24541 aacccaatct gtcggatttt gaaagcattc attagttagc tgtggttttt ggatttttca
    24601 ccatatcaac ggtatcaatt tataccgggt atgctttgaa tattcagcat atccgtctaa
    24661 ttctgctttt aaggttttat cttctaatga agtccttatc aacaaaagaa taacagctat
    24721 tgttgttggc aaaatgcacc aaaaagaggt tgtgattatt ggtaccgcaa ataaagaaac
    24781 agccattcct aaatatccgg ggtggcggat tattttatat aatcctttat cacaaacagt
    24841 gtgccctctt tctttttgaa ttctcaccat actggaaaaa aaattatttt ggtttcttgc
    24901 cgtcagaaag atactttgcc ctatggccaa aattgccact ccaatggcac tgatgaacca
    24961 gttaaaactt tttgtccata aaaacctgcc agagtctaac ccggctatta ttatcattat
    25021 taaataaacc atggccgata gtgctaatag ccatttatcc catgatttga taccttctcc
    25081 taattttgag cgttcaatta ataaatctgt attttttctg agtgttacaa cattcacaac
    25141 agtggtaagc gtgtttgctg ccagaaagac ccatccctgg taataatcaa gacggccagc
    25201 acttaaaaac aagatgattg taaatattgc cgttgtaatc agccctttca tcaatccttt
    25261 gaaagtttcc ataatttatt gcttttatat attacatggt tatttttaaa tagcccatcc
    25321 ttttacaagc gggacggtat gtttgcaaat tccctgctac cgctatgttt tatggctgct
    25381 tgtctgggcc cgtagttatt tatatctaaa ctaatcaaat ctccatatca aacaactttg
    25441 acagccttta taattcaagc ggaagctctt tgacaacgct ttgggttgag cgttgcccct
    25501 ttatatggga ggaagcccca attcatttaa atatttattg tgttcctccc tggctttgtt
    25561 aatttctttg tcaagttcgg ccaatttttt gtttacctct ttcaggtcaa ttatttcttc
    25621 gtcaaccgat gtgcttacat agcgtgaaat attcaggttg aattcgtttt tcccgatttc
    25681 gtccattgaa accctgcggg agtaacgggt ttcttcttta cggcctttgt aggtgttaac
    25741 aatcttgtca atgtgttcgg ggagcagggt gttttgtcgt ttcccctttt caaaatggtc
    25801 aacggcgttg atgaacaata catcgtcgaa ctttttgcac ttctttaaaa ccaatataca
    25861 aaccggaata cctgtagaga aaaacaagtt ggcaggtagc ccgattaccg tgtcaatgtt
    25921 tccgtcctct aataattttc tcctgatgcg ttcttccgat ccgcccctga acaaaacgcc
    25981 gtggggcaaa ataattgcca tggtcccctc tttactaagg aagtggaagc cgtgcaaaag
    26041 gaacgagaag tcggctgccg atttgggtgc aagcccgtaa cccttgaaac ggaaatcttc
    26101 gcccatggct tcggttggct cccaacgata gctgaaaggc ggattggcaa caacagcatc
    26161 aaattccaat tttctggcag ggttcatttc gttcaaaata tcccaatcgt tcagcagggt
    26221 gtcgccgtgg tggatttcaa actccgtgtc cttcacccca tgcaaaagca tgttcatgcg
    26281 ggctaagttg taggtggtga tgtttttttc ctgaccgaaa attttaccga ttgtgccccc
    26341 tgcaccggtc atttttttgc ggacattgag caacagcgaa cccgaaccac aagcaaagtc
    26401 caacacccgt tcaatctttt tcttttcgcc tttg
//
LOCUS       DAOSPF010000050        25583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1927_length_25583_cov_7.233156, whole genome shotgun sequence.
ACCESSION   DAOSPF010000050 DAOSPF010000000
VERSION     DAOSPF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_25583_cov_7.233156"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PK252_10245"
     CDS             complement(<1..985)
                     /locus_tag="PK252_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15128.1"
                     /translation="MTNTTNDQAKLKKELDKSKLKNRLYQNLFDNLNIGILVVARNQI
                     IAANNKSLHILGFTLDELFEKDVNAIFPLLQPDGRKSITIIDNAFKSINLIDAELTNF
                     QLLNKHKEEINCNVLVHTLILDGEKYIQLEITEVLTKQELSQRLREKNKQIETLSEER
                     ESLNEELRATLDELVEVNKQLAESEFWNKSIVDNMPLGLHVINDSITEHINEKYLDIA
                     GYDYDELIKKNMYDLALPEEHEKIRQFYEEFNKSSNSESAIDYWINDGHGRKKYIRNQ
                     FVKLSKEGRLMILVSDLTESKQRELEITNIRDRLEFAIEAGEINIWDIDLVG"
     gene            complement(1124..1645)
                     /locus_tag="PK252_10250"
     CDS             complement(1124..1645)
                     /locus_tag="PK252_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT15129.1"
                     /translation="MRPFIQFSSRFIQKSNEYYLAFKIKDESFAANFPKIVGFSKSFT
                     CFETASAHTLITHFAKIGERYLPILNLSKKLGFEASTELAVTPSRIVIFETELYDNKI
                     EFAIAYDELGEAFEIGQDKIETVQNDGSAYENIYVKGVHVHNKECMLIINIDKLIDID
                     DLIDIKVLEKQYK"
     gene            complement(1823..3877)
                     /locus_tag="PK252_10255"
     CDS             complement(1823..3877)
                     /locus_tag="PK252_10255"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT15130.1"
                     /translation="MMLLFRKGFGSGGIKENHSTFGKLPKKCYFRASETWTFFIMPPL
                     FKQKHLAFLLLLLYFCLSSTAFGSSVNQWPENKKSVDSINHLIRDAKDSASVYSLFAL
                     ATSIADKINYEAGLASAYDRLGCFLRNTSKYRQALEYHTKALTIANKINDISLQENIL
                     NNIGVVYRRINNTKLALEYHMKALMLAEKYNHFRTISISTNCIGNIYLSLNQYEEALK
                     CFRKVYPLEQKEDNRLGMAINMQNIGSVFRQMGQTDSALHYYRLSLKMNAEIWDKNGI
                     AICNSSIGSVLLMQNEVDEAMKCFNKALEIDKELKDKIYLAMCYNNLGNAWLMKGNPT
                     KAKDYFNNALETASAIGSKSECLKAYSGLSECLKKMKRFEQALKYAEIAKQYSDSLQN
                     EENVKNITQIRSQYDFEKKEQQITLLNKEKEASYLYNLLLATALVIFLTIAFFAILNI
                     RQKRKITRQELSLKEQKIHDLEREKQLIATQMVLQGEETERARLARDLHDGLGGLLSG
                     VKLSMAGIKNNPDGTENSQVDKALQLLDISIAELRRMAHNLMPESLVKYGLHEALSDF
                     CNSMDNTSCKVIFNFYGQSSRLDKKFENNLYRIAQELINNALKHAQATEIIVQLVQEH
                     NRINLTVQDNGKGFDTRILEKSKSSGIANIRSRVESLNGWLEITSNINQGTEITIEYL
                     YH"
     gene            4112..8050
                     /locus_tag="PK252_10260"
     CDS             4112..8050
                     /locus_tag="PK252_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15131.1"
                     /translation="MRKINKYYQLLIINLSFFLAFPFLLQGQHNIRFKHLTTDQGLSQ
                     NWIRCIIQDKDGFMWFGTGGNGINRYDGYDFKVYKNDPNDTNTITSNWINFLYIDKKD
                     RLWVGTQHGLNMYNKEKDNFIRFSYFQSEIITGIHESDNGQCYIVTGGNIFEVDVDHK
                     TVKPFCQHYEGCYGNIFRASLIDGDKLWVASLNGLFLFDLTNKTYKNFRHDSNNPASL
                     SDNVLQTIYKDTKGRIWTGTIKKGLSLISCSEKNFEQLCFINFTHNPLDATSISDGGI
                     WAILDDGDGNLWIGKENGSMDLLDLKSFEKGKTIFRHFENNPFDQTSLSNNSINTFFK
                     DNQGTVWVGTYSGGINYYNKRMFKFDHFKHIPNEKNTINNNTINVFMEDGDELWIGTE
                     NGINVLNRKTGKYKQFTYSPKNPRSIGSNAISAIFKDSHNNIWVGTWAGGLNLYNRQT
                     ESFTRFLQDNNNPYSISCNNVFGITEDKDGYLWLATMSGGLNKYNRKTGKFKAFHFRA
                     GDNKSITGDWVQTILETSTNELWIGTSDGVDIFDKKTETSINFKNDSANTKSISYNGA
                     NTIFEDSKHNIWIGTDGGLNLFVRKDSSFIYYREQDGLPNNAIKSICEDDKGNLWIST
                     NYGISKFVDAVNLPAKAVFKNYTKNDGLQSNEFSRRASLKTSDGKLYFGGINGYNVFH
                     PDSLKDNPQAPNIVFTDFLLYNKPVAIGTKDSPLKQHIGVAKEIVLSHLQSVFSIKFA
                     ALNYISPQNNQYAYILEGFEKDWNYVGNKREVTYTNLNAGKYVFRVKASNNDGLWNEA
                     GVSIKIIVLPPWWKTLWFKILIYALLATLVFLFINFRLKFYRKQNVKLSAMVKERTKE
                     LEEANVYLEEKQEEINNQNEELMSQRDALEEANKKLEKQKLQILEQNKELNLHRNELE
                     NLVEERTRQLIIAKEKAEESDRLKSSFLANLSHEIRTPLNAIVGFSTMALEETFTDEE
                     KAEHKQIIQKSSDNLLALISDIIDFSKIESGNIDIVFREVPMTHIIKNIGDIFKTQLN
                     RQQMGVIHDLQLKTDFPDELRNISLETDEMRLLQILSNLTHNAIKFTNKGEIQIGCKL
                     IDDGKMAEFYVKDTGIGISKEHHEIIFERFRKIENDKSMLHRGSGIGLSISQHLVKML
                     GGYIRVESEPNLGSTFFFSIPVKKAFSISTHNESTANNNKEMTDFKKALVLVAEDDLA
                     NYIIIEKFLQKHNIQVLRAVNGVHAVSMALSESDIQLILMDIKMPEMDGVEALHRIRE
                     KGITVPVVAQTAYALAHEVVKLKQEGFDDYITKPLDFAQILKILQKYLSP"
     gene            8099..8908
                     /locus_tag="PK252_10265"
     CDS             8099..8908
                     /locus_tag="PK252_10265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT15132.1"
                     /translation="MFGYIRQLVKNTFIEAEASSADYQSKQKTVDKKTIQICCIVAFS
                     VVGVRYFGDTTFVMQFIQGAGLDTLHYSFETLFYRSADASLYRLCWWVASIVGFYLVV
                     PAIFIKFVWKEQMRAYGFKLRGAFKDYRMYFIMLAVMMPLVLWFSTTASFQLRYPFYK
                     PIAGQPLFPKFVIWEFVYFFQFIAVEFFFRGFMVHGTKQRFGFYAVFVMMIPYCMVHF
                     GKPLPETIAAIAAGIMLGILSLKSRSVILGIAIHYSVAISMDLFALWQLRH"
     gene            9024..12392
                     /gene="ileS"
                     /locus_tag="PK252_10270"
     CDS             9024..12392
                     /gene="ileS"
                     /locus_tag="PK252_10270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT15133.1"
                     /translation="MKFAEYKNFNLSEINKDILARWQQLKVFEQSIEERAENPAFVFY
                     EGPPSANGMPGIHHVMARTIKDIVCRYKTMKGFQVKRKAGWDTHGLPVELGVEKALGI
                     TKEDIGKKISVEDYNHACRKDVMKYTREWEELTKKMGYWVDMSDPYITYDNKYIETLW
                     YLLKQLYNKGLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTIVAQFKVVHNE
                     KSEFLFANADTDVYFMAWTTTPWTLPSNTALAVGANIDYIQVRTFNPYTGIPITVILA
                     KDLKEVYFPEKNKDLAIEEFQQGQKNLPYKVLGAYKGSQLEGIRYEQLMPYQQPSTGD
                     AFRVICGDFVTTEDGTGIVHIAPSFGADDFRVAAQNGIGALTLVDKQGKFVDGCGDLS
                     GRFVKNAYDPNLKEEDESVDVYIAVKLKTENKAFRIEKYVHTYPHCWRTDKPVLYYPL
                     DSWFIKTTAMRERMIELNNTVNWKPDSTGTGRFGKWLENLQDWNLSRSRYWGTPLPIW
                     RTEDGKEELCIGSVSELKAQIELAIKAGLMKSNPLSNYKEGDNTKENYNSFDLHKPFV
                     DNIILVSPSGKAMRREPDLIDVWFDSGAMPYAQLHYPFENKELVDKKKYYPADFIAEG
                     VDQTRGWFFTLHAIATMLFDSVAFKNVVSNGLVLDKNGNKMSKRLGNAVDPFDTIEKH
                     GSDPLRWYMITNASPWDNLKFDIAGVDECNRKFFGTLYNTYSFFALYANVDGFSFKEA
                     EIPVAQRPEIDRWIISLLNTLVKEVDEYYADYEPTKAGRAISNFVSENLSNWYVRLNR
                     KRFWGGEYNQDKIAAYQTLYTCLATIAKLMAPIAPFFSDKLYTDLNNASQKEKNISVH
                     LALFPAVNGQYIDKNLEYRMDIAQRISSMVLSLRRKVNIKVRQPLQKILVPILDQRFV
                     EQFEAVKSLILTEVNVKEVEYLKDTKGFLTKRIKPDFKALGPRYGKLMKQIANAIAGF
                     SQDDIYAFEAQGTWELTIEGQKVSLVITDVEITSEDIPGWLVATEDKLTVALDVTITD
                     ELRHEGIARELINRIQNIRKESGFEVTDKIIIQLQRHEAINQAVEVHRQYIASQTLAA
                     SLELVESIVNHDSKAIEIDEEIQTNIRIIRNSNN"
     gene            12423..12803
                     /locus_tag="PK252_10275"
     CDS             12423..12803
                     /locus_tag="PK252_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT15134.1"
                     /translation="MAEKTRYTDEELEEFKKLIIDKLEQARKDYDLLKSTVMHTEGND
                     IEDTSPTFKVLEEGAAVLSKEEAGRLAQRQLKFIQHLQAALVRIENKTYGICRETGKL
                     IPKERLMAVPHATLSIEAKQGSPR"
     gene            12941..13540
                     /locus_tag="PK252_10280"
     CDS             12941..13540
                     /locus_tag="PK252_10280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOT15135.1"
                     /translation="MKKGKIAILTILLVLLVDQIVKIWIKTHMVLGEEYRITNWFIIH
                     FTENNGMAFGFELGGSIGKVLLSSFRIVAVGAIGWYLWKLVKDKAPTGLIISIALILA
                     GALGNIIDSLFYGLIFDHSYYQVAHIFPENGGYAGFLHGRVVDMFYFPLIKTHFPAWF
                     PIWGGQEFEFFRPVFNLADSAITTGVAIILLFQRKYFKD"
     gene            13633..14160
                     /locus_tag="PK252_10285"
     CDS             13633..14160
                     /locus_tag="PK252_10285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT15136.1"
                     /translation="MKAEYTIKQVQVYYLRMDNKPAFVISEGKGLSVEELPKPIDIDG
                     YLKLYTSVGKEYNWIDRLEMPKDLLINIINNSKTHVLLFKVNGTLAGWAELVEGREYV
                     ELLYFGLLPEFVGKGLGKSFLAMNINKAWELNPAWIQLNTCSLDHPNAINVYKSLGFV
                     EYKREFEMRKVFLRE"
     gene            complement(14168..14485)
                     /locus_tag="PK252_10290"
     CDS             complement(14168..14485)
                     /locus_tag="PK252_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15137.1"
                     /translation="MSENTPIQYELKRIKPVSLGKVIALWSALIGFIVGLIVGFVMFV
                     IGEFISDVSGWGPLLGIGGFLLVPLVLAALGFIFGVVAAFLYNFFARHSGGIILELEE
                     KLN"
     gene            complement(14488..15480)
                     /locus_tag="PK252_10295"
     CDS             complement(14488..15480)
                     /locus_tag="PK252_10295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT15138.1"
                     /translation="MEKVKVFVTGGTGFIGMHLIKQLLDEGFEVHAIYRSESKIAGMK
                     HNGVTWFKGDILDLDVLKTAMQGCLQVYHVAAYASAWEEKPGDFKKYNVQGTINIIET
                     AREAKVEKIVVTSTAGVFGPSIVKFIDEQSISPLPHFTGYELSKAESENLIAQYVQKG
                     MNIVIVNPTRVFGPGLLSESNSVTILIKKYINGKWHFVPGNGKSIGNYAYVEDVAKGH
                     ILAMKNGKAGQKYILGGENLSYNDIFQQLDALTGKRFAKFSIPLWLMLFVAHFLLLLN
                     RLIKLKPAFTPAHVRKFNYNWELSVDKAVSQLGYQVTPFSQALKKTVEYLSKTK"
     gene            complement(15468..16154)
                     /locus_tag="PK252_10300"
     CDS             complement(15468..16154)
                     /locus_tag="PK252_10300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT15139.1"
                     /translation="MSQTALSVAIVTFAFLAYLSFQGNKAIIELLTRKYGNEGGQWRH
                     IVAVRLFGALIFGGTSVISAYAIGFSAGYIGIAIAPTMLSLVFILCFGVLMVIVNFFA
                     ARTDGNLAMYPQIRIEKFSTSILIVSAVTWLLYLLGYEIMLRGFLFAFCMAEFDLKSA
                     ILINISIYALIHLPKGMKETIGSLPMGAVLCYITYKTGSIWAAFFIHATLALSNEWFS
                     IKYHKLLWKK"
     gene            complement(16165..16470)
                     /locus_tag="PK252_10305"
     CDS             complement(16165..16470)
                     /locus_tag="PK252_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOT15140.1"
                     /translation="MIKHVVSWKIKDEHEGMSKTEVLEKIKSMLLALKGKVDVIRTIE
                     VGINDPLIDASNNDLVLIVTFDNAKDLDTYQKHPEHKLVGAFIGEVRLSRSCVDFEF"
     gene            complement(16482..16856)
                     /locus_tag="PK252_10310"
     CDS             complement(16482..16856)
                     /locus_tag="PK252_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT15141.1"
                     /translation="MMLTAKQVILLVITGLLAGMVSGALGVGGAIIIIPFLVFFLGLS
                     QQQAQGTSLMILVFPVGIFAVMNYAKNGYVNYPFALIVILAFLLGSYIGSVFAVNLPE
                     KALQKGFAILLLFIGIKMLLGK"
     gene            16975..17685
                     /locus_tag="PK252_10315"
     CDS             16975..17685
                     /locus_tag="PK252_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOT15142.1"
                     /translation="MFDSHSKSKKHIVVLTGSGISAESGLKTFRDSGGLWETYDVMEV
                     ASIDGWYKNKELVLRFYNERRKQLEQAAPNKAHLLLKKLEDNFKVSIITQNVDNLHER
                     AGSKNILHLHGELTKVRSTKDPNYIVDIGYKEIKPGDKCPRGGQLRPHIVWFGEAVPA
                     MEEAIGLTQEADIFVIIGTSLNVYPAAGIVDYVKPEVPIFFIDPKPANTHYRQNLEII
                     AEKASTGMEILLKKLEVS"
     gene            17950..21006
                     /locus_tag="PK252_10320"
     CDS             17950..21006
                     /locus_tag="PK252_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15143.1"
                     /translation="MGEKKHNDNFHLFTVLKKYSKEDFATQMKIKFIFLLLVSFIILQ
                     TIIIVTTGFIQLKTTEDHALKWPVIMVEVSLLLTFCISLALLIKGFYKIVSQAILILT
                     FVCIWVVMFLDTSNSVTRLDTVVYILLVFLALPIFSFYKKWDIFLYTIANLLTFGIFV
                     YVFFSQNPHLSIYAYDYISDNSITLIIAGILAYNNLRIHSISLQKANEDLRTRVLAEE
                     MLLQSEKKYREITELLPQTVFEAELSGKLTYVNQNGFKQFGYNEEDFRQGLNVINTIH
                     PDDRQRTLNNIQKIIKGTPTTGNLYRAMRKDGTIFPVQIFTSVIYHDSKPVGFRGVIT
                     DMTERVHFEDEIKKSRDQFQSLVDNIPGIVYRCLFDKNLTIQYMSPETEKLCGYTAQN
                     FINNNKFSFSNIIHPEDNDKVISTIEKAVKNKTSWETEYRIIHKDKHILWVNEKGRAI
                     YKDSGQVEYLDGLILDITQRKNAEEALLISEERFKQLFYNAPVALAFINDKGDIKGIN
                     NTFINTLGYTIEDLPSLDIWWKKAYPNKDYRKEIVFQWGQKTKRAIANGQDISPDEYY
                     VTDKKGQGHYMLIGASIQKTSTLFSLVDITERKKAEEALKQSEERFKALIEFLPYSVM
                     VTDLQERYVMVNKVFCQSMGYSPEEIIGKTATELDIITKYENPQEQKKLLFAQGHIEN
                     VEMEVTIKKTTRLYIYFSAKVIQMNNEPYLLCTSVNVTEKKKIEDELENYRKNLEMLV
                     QERTEELATANEELAAANEELSSTNEVLLQQREELEEALKNLQKAQLQLIESEKMASL
                     GVLAAGVAHEINNPLNFIQGGVVALENYLQENYQNNTKETTTLFNAINTGVERVSKIV
                     NSLGRFSRQSGTNDELCDIHFILDNCLVMLQNTIKYKASITKQYTTQPFRLLANEGKL
                     HQAFLNIISNAAQAIEKNGEITITTSVENNLLTISVKDTGKGIKQEYLGKIFDPFFTT
                     KDPGKGTGLGLAITYKIIKELGGNISAESNENTGTTMIIELPVSTK"
     gene            complement(21018..21992)
                     /locus_tag="PK252_10325"
     CDS             complement(21018..21992)
                     /locus_tag="PK252_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOT15144.1"
                     /translation="MPKKHFLILLLIGIPLLVKSQQTAVYSQYMDNAFVFNSALAGDV
                     GLTKFSLISKQQWMGMDLAPRTFSLSAQTRLLLRKFQIKSRPLKKNKMLATRSGRVGV
                     GLNVTNDRNGYFQNTTLTGSYAYHIPFTNSQLSLGLSGSLSQLKIDKSGIVFREAGDP
                     KEAFVEEPAYAPDIGVGIQYKDFESYYFGFSISNIIQTNIRFGNTTLNYNLKRHYYLM
                     AGYRKSFTNTSYIDLTTLAKSTELLMPQIDLSAKVVIKELYWLGLSYRSTTTAIFLAG
                     VYFRNFLVGYAFDYDFNSFNKVTVGSHELNLSLWFGDSSRRYKWIRRF"
     gene            complement(22113..>25583)
                     /locus_tag="PK252_10330"
     CDS             complement(22113..>25583)
                     /locus_tag="PK252_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT15145.1"
                     /translation="PVTSSVATSGIRYKWTVIDNSAGDITGFSNSDMTGNTIGSSIVQ
                     TLNNSSSSVKNIIYRITPWALNNQGDNTCSSPSIDITVYVNPTPRMSVTSNDTLFCNT
                     NTALFTLLSKNGVVMGNKYYYIEVSYDAAKIQVSGLQNVYAPIADFQHIYTNITTEMQ
                     PVTYKFTPVFNNPKGNDPADFSCAGVDTTFTRYILPTLKAELTAKEYVGGWNEPCFGD
                     AKGEIYVKLTGGMTKYSTPMSPLSASYVWKKNDNNFITTSDTTIKNLTIGNYRVDITD
                     THGCLANGSITLIQPEKLIASYIKVSEAECTALEKGMIVARQSGGTPDYTYLWLRGTL
                     PYSTNDTIDKLSIGDYTIIITDANACKASAVAFFRPSQGGLNVSTNPAYYGEYNISCP
                     GKSDGAFTPYQYPSDANIQYTYEWSKLNENTGLFTVIYSGPQGSISGLSKGVYKLFVK
                     NSLGCTSFPKTDTLKEPSRVTINPLVSKPYENEVFGVSCHNSKDGSIQLNPEGGHGTY
                     QYFWTDSTGTFSASTKDIANLDAGKYYLRLADTSLRNTGKIYMHMHCDYYDTIVLTKP
                     AKLVVDHRQSDYNGYGIDCNGSNTGYAKALANGGFGKYSYTWSKVGIGNLPFSGDSIS
                     KLAAGQYVLTVGYGKGCNEKDTFELTQPLPLSNNAFLSNYNDSNISCFGLKDGFIHTT
                     ISQGVPGYTYNWQNLPSNVTIGTSNNLDNIGAGNYKLTVRDKNNCSHVWNYHLSEPDK
                     LGASITSIRQTCNGVKDGKAFISNAFGGIKPYMYKWLNGSYDTVSSVNNLVEGSYNVV
                     MQDKNGCFVNLPFTILEPNPLNVELVIQSDYNSRSISCYNASDAKLYANVMGGTRPYI
                     YHWSRLDNIVQNFSSDSVQYGLSAGKYGVIVSDSSNCHGSSTITITQPDSLQMSLDLT
                     NVNCYLGDDGKARATVIGGTRPYRFMWNFVEGDSLAEGLRAGNFNVDVYDANNCYKNG
                     KFVLTQPDSLRLTVSVKTDPYCPATFDGQLKVESAGGVAPYQFNWNTGSTSDALYDIQ
                     QGQYLVTVTDAHSCTQTMAIELKAKNNDCVSIPLAFSPNKDGYNEKWNILAGDPRNYQ
                     ILSELYPKAVVEIYNRWGTLVYRSERGYKEEWDGRSNMKMDLPMDSYYYSIDLGNGSP
                     KIVGIVSIIR"
BASE COUNT         8217 a         4974 c         4860 g         7532 t
ORIGIN      
        1 ggccaacaag gtcaatgtcc cagatattta tttctcctgc ttcaatggca aattccaacc
       61 tgtcacggat attggtgatt tcgagttctc tttgtttgct ttcggtgagg tcagagacca
      121 aaatcatcaa acggccttct ttactgagct ttacaaattg gttccttatg tatttctttc
      181 tgccatggcc atcgtttatc caataatcaa tggcagactc agagttactg cttttgttga
      241 attcttcgta aaactgtcgg attttttcat gctcttccgg caaagccaga tcgtacatat
      301 tttttttaat cagctcatcg tagtcgtatc ctgcaatgtc gagatatttt tcgttgatat
      361 gctcggtaat gctatcattg ataacatgta agccaagggg catattgtcc acaatagatt
      421 tgttccaaaa ctcgctttcg gcaagctgct tgttaacttc aaccagctca tcgagggtag
      481 cacggagctc ttcgttgagg ctttctcgtt cttcgctaag ggtttctatc tgtttgtttt
      541 tttcacgaag gcgttgcgat aattcttgtt tagtaagtac ttctgttatc tcaagctgta
      601 tatatttttc tccatcaagg attaaggtat gtaccagtac attgcagttg atttcttctt
      661 tgtgtttgtt gagtagttgg aaatttgtaa gttcggcatc aataaggtta atagatttaa
      721 aagcattgtc aatgatggta attgatttgc ggccatcggg ctgaagaaga gggaaaatgg
      781 cattaacgtc tttttcaaaa agctcatcaa gtgtaaaacc aagaatatga agggacttat
      841 tgttggcagc aatgatttgg tttctggcaa ctacaaggat accaatattc aggttgtcaa
      901 aaaggttttg gtacagcctg tttttgagtt tagacttgtc cagctctttt ttcagtttag
      961 cctggtcatt ggttgtattt gtcatgatgt atgaaaaaca ttatttctag tatcgtaaat
     1021 atactaataa aatattaaaa aaaatgccct gcataatttt atgcaaggca tttttcaatc
     1081 atccttaact aaacactaac ccatgaaaag agaatgcaat tttttattta tactgttttt
     1141 ctaatacttt gatgtctatc aaatcgtcaa tatctataag cttatcgatg tttatgataa
     1201 gcatgcactc tttgttatga acatgtacgc ctttgacata tatattttca taagcagaac
     1261 catcattttg gacagtctct attttgtcct ggccaatttc aaaggcttct ccaagctcgt
     1321 cataagcaat ggcaaactct atcttattat cataaagttc ggtttcaaaa atcactatgc
     1381 gactcggtgt tacagcaagt tctgtagatg cttcaaagcc tagttttttc gacaggttga
     1441 gtattggcaa atagcgttcg cctatttttg caaaatgagt aatcaatgta tgtgctgaag
     1501 ctgtctcaaa acaagtaaaa gactttgaaa atcctactat tttaggaaag tttgcagcaa
     1561 aagactcatc ttttatttta aaagcaagat agtattcgtt gcttttttga atgaatctcg
     1621 aactaaattg aatgaagggg cgcatgattg aaaagattta ttgttagata caaaaatagt
     1681 atatccaaaa taacatttca atcagcgttt ttactgaaaa gttgtaaaaa aaccctcaat
     1741 ttttttggag gtttttagca aaaaaatggc acaaaacact catcgcatta gccgttatgt
     1801 gcaaaaaaac tggcaagtgg atttagtgat acaaatattc aatagtaatt tcggtacctt
     1861 ggttgatatt acttgttatc tcaagccatc cattgagcga ttcgactctt gagcggatgt
     1921 tggctatgcc tgaactttta gatttttcaa ggattctggt gtcgaaacct ttaccgttgt
     1981 cctgaacggt gaggtttatg cggttatgct cctgcacaag ctgaacaata atctcggtag
     2041 cttgtgcatg ttttagtgca ttgttaatta attcctgggc aatgcggtac aggttatttt
     2101 caaacttttt gtccaacctg ctgctttgcc cgtaaaagtt gaaaatgact ttacaggaag
     2161 tattgtccat gctattgcaa aaatcagaca aagcttcatg taacccatat tttacgagac
     2221 tttcgggcat cagattgtga gccatgcgcc tgagctcggc aatagaaata tccagtagtt
     2281 gaagagcttt gtccacctgg ctgttttcag taccgtctgg gttgtttttt atgcccgcca
     2341 tagaaagctt gacgcccgaa agcaagcctc caagcccgtc gtgcaaatcg cgcgcaagac
     2401 gggctctttc ggtctcctcg ccttgcaata ccatttgtgt tgctatcagc tgtttttcgc
     2461 gttctaaatc gtgtattttt tgttctttca gcgatagttc ctgccgtgtg attttgcgct
     2521 tctgcctgat attgaggatt gcaaaaaatg cgatggttaa aaatattacc aacgctgtgg
     2581 ccaaaaggag gttgtaaagg tatgatgctt ctttctcctt atttagaagg gtaatttgtt
     2641 gttccttctt ttcaaagtca tattgcgagc gtatttgcgt gatgttttta acgttttctt
     2701 cgttttgtaa gctgtctgaa tattgttttg ctatttcggc atattttaaa gcttgttcaa
     2761 aacgtttcat tttttttaaa cactctgata ggcctgagta tgcctttaag cattcgcttt
     2821 ttgacccaat agcactggct gtttctaatg cattgttgaa ataatctttc gcttttgtag
     2881 gatttccttt catcaaccat gcattgccca gattgttgta gcacatggca aggtatattt
     2941 tgtcttttag ttctttatct atctctaatg ccttattaaa gcatttcatg gcttcatcta
     3001 cctcgttttg catcagtaat actgaaccta tagagctatt gcatattgca atgccatttt
     3061 tatcccatat ttcggcattc atttttaacg aaaggcggta ataatgcagt gcgctgtctg
     3121 tttgtcccat ctgccgaaat accgagccta tgttttgcat gtttatggcc attcccaacc
     3181 tgttatcttc tttttgttcc aacggataca cttttcggaa acatttcaaa gcttcttcat
     3241 attggttgag gcttaaataa atattgccaa tgcagttggt tgaaatgctt atagttctga
     3301 aatgattgta tttctcagcc agcatgaggg ctttcatgtg atattccaag gccagctttg
     3361 tgttgttaat gcgtctgtaa actacgccaa tgttgttgag tatgttttcc tgaagtgaaa
     3421 tatcattgat tttattggca atagtgagcg ctttggtgtg atattcgagc gcttgcctgt
     3481 actttgaagt attgcgcaaa aaacaaccta aacggtcgta tgcactggca agtcctgcct
     3541 cataattaat tttgtcagca atagatgtcg ctaaggcaaa caaagaataa accgaagcag
     3601 aatctttcgc atcgcggata agatggttta ttgaatctac acttttttta ttttcaggcc
     3661 attggtttac agaagagcca aatgctgttg aacttaggca aaaatatagc aacaataaca
     3721 gaaaagcgag atgcttttgt ttaaataaag ggggcataat aaaaaaagtc caggtttctg
     3781 atgcccgaaa gtaacatttt ttaggcagtt tgccaaaagt tgaatgattt tcttttatcc
     3841 cccccgaacc aaatcccttt ctgaaaagca acatcatact gctttgattt aatagaaata
     3901 agcatttttt ctcctttttt ttgattaact ttaattggaa atcaccattg tccgatataa
     3961 aatttagtat tagggctaaa agcccaatta cttcagagct ttgactaact ccagccttaa
     4021 ggccggggtt acaaacgcta acttactgac ggattttagt cctgatattt ttaccggaca
     4081 acagtggttg gaaatatatt tataatttat aatgagaaaa ataaataaat attaccagct
     4141 tctgattata aatttgtcat tttttctggc ctttccattt ttattgcaag gccaacacaa
     4201 tatcaggttc aagcatctta ccacagacca ggggctttcg caaaactgga taaggtgcat
     4261 catccaggac aaagacgggt ttatgtggtt tggcactggc ggcaatggca tcaacaggta
     4321 tgatggctat gattttaaag tatataaaaa tgaccccaat gatacgaata ccattactag
     4381 caattggatt aactttcttt acatagataa aaaagaccgc ttatgggttg gcacccagca
     4441 tgggttaaat atgtacaata aagaaaagga taattttatt cgtttttcat atttccaatc
     4501 tgagattatt actggcatac atgaatcaga taatgggcaa tgctatatag taaccggagg
     4561 caacattttt gaagttgacg ttgaccataa aacagtaaaa ccattttgtc agcattatga
     4621 gggctgttat ggcaacatat tcagggcaag ccttatagac ggggataagc tgtgggtagc
     4681 aagtctcaat ggtttgtttt tgtttgacct gacaaataaa acttataaaa acttcaggca
     4741 tgacagcaac aaccctgcaa gcttgtctga caacgtactg caaacaatat ataaagacac
     4801 caaaggaagg atctggacag ggacaattaa aaaagggcta agcctgattt cgtgttcaga
     4861 aaaaaatttt gaacaactgt gttttatcaa ttttacacac aatcctctgg atgccaccag
     4921 cattagcgat ggcgggatat gggctatttt agacgatgga gatggcaatt tatggattgg
     4981 taaagaaaac ggcagtatgg acctactcga cttgaagagc tttgaaaagg gcaaaaccat
     5041 ttttaggcat tttgaaaaca acccttttga ccaaaccagc ctttcaaata attctatcaa
     5101 cacgttcttt aaagataacc agggaaccgt atgggttggg acctacagcg gaggaatcaa
     5161 ctattacaat aaacgaatgt tcaaatttga ccatttcaaa catatcccta atgaaaaaaa
     5221 caccatcaac aacaacacca tcaacgtttt catggaagat ggagatgaac tctggattgg
     5281 caccgaaaac gggattaatg tactaaacag aaaaactggg aaatataaac aatttaccta
     5341 cagcccgaaa aatccccgaa gcattggttc aaatgccata agcgctattt tcaaagacag
     5401 ccacaataat atatgggtag ggacatgggc cgggggcctc aacttataca accgacaaac
     5461 tgagtctttt acccgctttt tgcaagataa caacaacccg tatagtatca gttgcaacaa
     5521 cgttttcggc ataaccgaag acaaagacgg ttatttatgg ctggccacca tgagcggggg
     5581 cttaaacaaa tacaatcgta aaacaggtaa attcaaagct tttcatttca gggccggaga
     5641 taataaaagc attaccggcg actgggtaca aacaatactc gaaaccagca ctaacgagct
     5701 atggataggg acttcagatg gtgttgatat ttttgacaaa aaaacagaaa cttccataaa
     5761 cttcaaaaac gactcggcca atacaaaaag catcagctat aatggggcaa acactatttt
     5821 tgaagacagt aaacacaata tttggatagg gactgatggc gggctaaacc tctttgtcag
     5881 aaaagacagc tcatttattt attaccgtga acaagacggc cttcccaata atgctataaa
     5941 aagtatatgc gaagatgaca aagggaatct ttggattagc accaattacg gcatttcgaa
     6001 atttgttgat gcagtaaacc ttcctgccaa agcggttttt aaaaattata caaaaaacga
     6061 cggacttcaa agcaatgagt ttagcaggcg ggcttcttta aaaacttcag acggaaagtt
     6121 atattttggt ggcatcaacg ggtacaatgt ttttcatcct gacagcctga aagacaaccc
     6181 gcaagctccc aacatagtat ttacagattt tttgctatac aacaaacctg ttgccatagg
     6241 gacaaaagat tcaccgctca aacaacatat tggcgttgcc aaagaaatcg ttttatcaca
     6301 cttacaatca gtatttagca taaagtttgc agccttaaat tatatttcgc ctcaaaacaa
     6361 tcaatatgcc tatatattag aaggttttga aaaagattgg aactatgtag gcaataaaag
     6421 agaagtaact tataccaacc tcaatgctgg caaatatgtt tttagggtaa aagccagcaa
     6481 taatgacggg ctatggaatg aagccggggt ttcgataaag attattgttt tgcctccctg
     6541 gtggaaaaca ctatggttta aaatattgat atatgccttg ttagctacat tagtttttct
     6601 ttttatcaat ttcaggctta aattctatcg caaacaaaac gtcaagcttt ctgcaatggt
     6661 caaagagcga acaaaagagc tggaagaagc aaatgtgtat ttggaagaaa aacaggagga
     6721 aataaataac caaaatgaag agctgatgag ccaacgcgat gccttagaag aagcaaacaa
     6781 gaagctcgaa aaacaaaagt tacagatact cgaacaaaac aaagaattaa accttcaccg
     6841 caacgaactc gaaaaccttg ttgaagaacg tacccgccag ctcatcatag ccaaagaaaa
     6901 agctgaagaa tcggacaggc tcaaatcttc ctttctggcc aatttatcac atgaaatcag
     6961 gaccccttta aacgctattg ttggattttc aaccatggcc cttgaagaaa catttaccga
     7021 tgaagaaaaa gcagagcata aacaaatcat tcaaaaaagc agtgataatt tgttggcctt
     7081 gatcagcgac attatcgatt tttcaaaaat tgagtcgggc aatatagaca ttgtttttag
     7141 agaggtgcca atgacccaca tcataaaaaa tattggcgat atttttaaaa cacagctcaa
     7201 ccggcaacaa atgggagtta tccacgattt gcagttaaaa acagattttc ccgatgaatt
     7261 acgcaatatc tcccttgaaa cagatgaaat gcgcttatta caaatattgt ctaacctgac
     7321 gcataatgcc ataaaattta ccaacaaagg agaaattcaa attggctgta agctcattga
     7381 tgatggaaag atggcagaat tttatgttaa agataccggt ataggcatta gtaaagaaca
     7441 ccatgaaatt atttttgagc gtttcagaaa aattgaaaat gataagtcaa tgcttcaccg
     7501 ggggtctggc ataggacttt ctatctcgca acatttggta aagatgttgg gaggctatat
     7561 cagagtagag tcggaaccaa atttaggctc aacatttttc ttttctattc cggtaaaaaa
     7621 agccttttca attagtacac ataatgaaag tactgcaaat aataataaag aaatgacaga
     7681 ttttaaaaaa gcactcgtat tggttgctga agacgatttg gcaaattata ttattatcga
     7741 aaagttctta caaaagcaca acatccaggt actacgcgcc gtcaatgggg ttcatgccgt
     7801 cagtatggca ttgtccgaaa gcgacataca acttatcctg atggatataa aaatgcccga
     7861 aatggacgga gtagaagcat tgcaccgaat cagggaaaaa ggcattactg tacctgtggt
     7921 agcccaaaca gcttatgctt tggcccacga agtagtcaaa cttaaacaag agggctttga
     7981 tgactatatt accaaaccgc ttgattttgc acaaattctt aagatattgc aaaaatattt
     8041 gagtccgtaa atattgcagc tataactata tttgcagcaa atcaatgtac aataaacaat
     8101 gtttggctac atcaggcaac tggttaaaaa caccttcatc gaagcagaag catcctcagc
     8161 tgattatcaa tcaaaacaaa agactgttga caaaaaaaca atacaaatct gttgcattgt
     8221 agcattttcg gtagtcgggg tacgttattt tggcgataca acctttgtaa tgcaattcat
     8281 tcaaggtgcc gggcttgata cattgcacta tagctttgaa acactttttt atcgttcggc
     8341 agatgcttct ttgtaccgtc tttgttggtg ggtagcaagt atagtcgggt tttacttagt
     8401 agtccctgct attttcatca agtttgtatg gaaagagcag atgcgggctt atgggttcaa
     8461 gctccgtggc gcttttaagg actatcgcat gtatttcatc atgcttgccg tgatgatgcc
     8521 gctggtgctg tggttttcga ccactgcaag ctttcaactc aggtatccgt tttataaacc
     8581 cattgctgga caacctttgt tccctaaatt cgttatctgg gaatttgttt atttttttca
     8641 gtttattgcc gtggaatttt tctttcgagg ctttatggta catggtacca aacaacgatt
     8701 tggcttttat gcagtgtttg tgatgatgat accatactgc atggtacatt ttggcaaacc
     8761 actgcccgaa actattgcag ctattgcagc aggtataatg ctggggatac taagtttgaa
     8821 aagccgttcg gtaatcctgg gtattgccat ccattacagc gtggctatat cgatggattt
     8881 gtttgctttg tggcaattaa ggcactagcc ttttcaaatt ccccaaaaaa cttttttttt
     8941 ctccatggga accatatctt tgcatacttt ttttataggc aaaaattgta aaagcctcaa
     9001 agaacttaat aaagcgtgca gaaatgaaat ttgcggaata caaaaatttc aacttatcag
     9061 aaattaacaa agacatcctt gcccgttggc aacagctgaa ggtatttgaa caaagtattg
     9121 aagaacgtgc agaaaatcct gcttttgtgt tttacgaagg cccgccttct gcaaacggta
     9181 tgcccggaat tcaccacgta atggcacgta ctataaagga tattgtgtgc cgctacaaaa
     9241 ccatgaaagg ttttcaggta aaacgtaagg ccggatggga tactcatggc ctccctgttg
     9301 aacttggtgt agaaaaagct ttgggtatta ctaaagaaga tattggcaaa aaaatttctg
     9361 ttgaagatta caaccacgct tgtcgtaagg atgtaatgaa atatacccgc gaatgggaag
     9421 aactaaccaa aaagatgggg tactgggtag atatgtctga cccatacatc acctacgaca
     9481 acaaatatat tgaaacactc tggtatttat taaaacaact ttacaataaa ggtttattat
     9541 acaaaggcta cactattcag ccatattctc cggctgcagg tacagggctt agcacacacg
     9601 agcttaacca acctggctgc tatcgtgatg ttaaggacac tactatagtt gcccagttta
     9661 aagttgtcca caacgaaaaa tcggaatttc tgtttgccaa tgcagatact gacgtatatt
     9721 tcatggcttg gacaaccaca ccctggacat tgccttcaaa tacggcttta gctgttggag
     9781 ctaacatcga ttacatccag gttaggactt ttaacccata tacaggcatt cctattacgg
     9841 taatcctggc aaaagacctc aaagaggttt atttccccga gaaaaataaa gaccttgcta
     9901 ttgaagaatt tcaacaaggc cagaaaaatt taccttacaa agtattgggg gcctacaaag
     9961 gctcccaact tgaaggaatc cgttacgagc aacttatgcc atatcagcaa ccttctactg
    10021 gcgatgcttt cagggtaata tgtggcgact ttgtcaccac cgaagatggt acgggtattg
    10081 tacatatagc accaagcttt ggagctgacg actttagggt tgcagcccaa aatggcattg
    10141 gagcccttac acttgttgac aaacaaggca aatttgttga tggctgtggc gatttaagcg
    10201 gtcgttttgt aaaaaatgct tacgatccaa atcttaagga agaagacgag tctgttgatg
    10261 tttacatagc tgttaagcta aaaaccgaaa ataaagcttt ccgtattgaa aaatacgttc
    10321 acacataccc tcattgctgg cgtacagata aacctgtact atattatccg ctcgactcat
    10381 ggtttatcaa aaccactgct atgcgtgagc gtatgataga actaaacaat accgtcaact
    10441 ggaaaccaga ctctaccggt accggtcgtt ttggcaaatg gcttgaaaat cttcaggatt
    10501 ggaacctttc acgttcacgt tactggggaa ctccccttcc tatatggcgc accgaagatg
    10561 gcaaagaaga attgtgcatt ggttctgttt ctgaacttaa agcacaaata gaacttgcta
    10621 ttaaagccgg tttgatgaag tcaaacccgt tgtcaaatta taaagaagga gacaacacta
    10681 aagaaaacta caatagtttt gacctgcata agccttttgt agataatatt atacttgtgt
    10741 ccccttcggg caaagccatg cgccgcgaac cagaccttat cgatgtttgg tttgacagtg
    10801 gggccatgcc ttatgctcaa ttacactatc cttttgaaaa caaagagctt gttgacaaaa
    10861 agaaatacta tccggccgac tttatcgctg aaggggttga ccaaacccgt ggatggttct
    10921 ttacattgca tgccattgct accatgctct ttgactcggt agcatttaaa aatgttgttt
    10981 caaacggcct tgtgctggac aaaaatggca ataagatgtc taaacgcctt ggtaatgcag
    11041 ttgacccatt tgacaccatc gaaaaacacg gttcagaccc gttgcgctgg tacatgatta
    11101 ctaatgcatc tccatgggac aacctcaagt ttgacattgc cggcgttgac gaatgtaacc
    11161 gaaaattttt cggtaccctt tacaatactt attctttctt tgccctatat gccaacgtag
    11221 atggttttag ctttaaagaa gctgaaatac ctgttgcaca acgtcctgaa attgaccgct
    11281 ggattatttc gctgctcaat actttggtaa aagaagtgga cgaatattat gccgactacg
    11341 aaccaaccaa agcaggccgt gctatttcaa atttcgtaag tgaaaacctg agcaactggt
    11401 acgtacgcct aaaccgcaag cgtttctggg gaggagaata caaccaggac aagattgctg
    11461 cttatcagac attatataca tgccttgcta ccattgcaaa actgatggca cctattgcac
    11521 ctttctttag cgacaagctt tatactgact taaataacgc aagtcagaaa gaaaagaaca
    11581 tttcagtaca tttggcattg ttcccggcag ttaatggaca atatattgat aaaaacctcg
    11641 aataccgcat ggatattgct cagcgaatat cttcaatggt actgagcctt cgccgtaagg
    11701 taaatatcaa ggtacgccag ccgcttcaaa agatattggt tccaattctc gaccagcgct
    11761 ttgttgaaca gtttgaggct gtaaaatcac ttatccttac tgaggttaac gtaaaagaag
    11821 ttgaatatct caaagataca aaaggatttt tgaccaaacg cattaaacca gatttcaaag
    11881 cattagggcc tcgttacggt aagctgatga aacaaattgc caatgccatt gctggtttct
    11941 ctcaagacga catttatgct tttgaagccc agggaacatg ggaactgact attgaaggac
    12001 aaaaagtgtc attggtcatt actgatgttg aaattacttc agaagatatt cccggatggc
    12061 tggtagctac tgaagataaa cttacggtag cacttgatgt tactattact gatgaactcc
    12121 gccacgaagg tatagcgcgt gaattaatta accgtatcca aaacatccgc aaggaaagcg
    12181 gatttgaagt aacagacaaa attatcatcc agttgcaacg ccatgaagcc attaaccagg
    12241 ctgttgaggt tcatcgtcaa tatatagctt cacaaacatt ggcagcctct ctggagcttg
    12301 tagaatctat tgttaaccac gactctaagg ctattgaaat agatgaagaa atccaaacaa
    12361 atattcgaat tatacgtaat agcaataatt aatacagatt acttaaccaa aacttaaata
    12421 ctatggcaga aaagaccaga tatacagacg aggagcttga agaattcaaa aagttgatta
    12481 tcgataagct cgagcaggcc agaaaagact acgatctgct aaagagtact gttatgcata
    12541 ccgaagggaa tgatatcgaa gacacgtcac ccacattcaa agtattggaa gaaggggcag
    12601 cagtgctttc aaaagaagaa gcagggcgcc ttgcccagcg ccagttaaag tttatccaac
    12661 atttacaggc agctttagta cgtattgaaa acaaaaccta cggcatctgc cgcgaaactg
    12721 gcaagctcat ccctaaagaa cgcctgatgg ctgtacctca tgctactctg agcattgaag
    12781 caaaacaagg ttcgcccaga tagcattcga gtgtattgct ttgaaatgct gtaaaatata
    12841 ctacttcttt gttatgcatt tttaacaaag aagtagtttt ttattttgat acctttgaca
    12901 ggtttatatt tttggatatc agataatttt tgtttgaaaa atgaaaaaag ggaaaattgc
    12961 cattcttacc attttgttgg ttttattggt agatcaaatt gtaaaaatat ggattaaaac
    13021 ccacatggta cttggcgagg aatatcgtat cacaaactgg tttatcattc atttcactga
    13081 aaataatggc atggcctttg ggtttgaatt aggtggttca atagggaaag tactacttag
    13141 ttcgtttaga attgtggcag ttggcgctat tggttggtat ttgtggaaat tggttaaaga
    13201 taaagcccca acaggtttaa tcatcagcat tgcacttatt ttagccggcg ctttgggcaa
    13261 tattattgac agcttatttt acggactaat ttttgaccac agctattatc aggtagcaca
    13321 catatttccc gaaaatggcg gatatgctgg ttttctgcat ggcagggttg tggacatgtt
    13381 ttattttcca ttgataaaga ctcattttcc tgcctggttc cctatctggg gaggtcagga
    13441 gtttgaattt ttcaggcctg ttttcaacct tgcagattct gctattacta ccggcgtagc
    13501 cataatacta ttatttcaaa gaaagtattt caaagattag cttgttcatt tattttatgg
    13561 ccaattgttg ctaaaaatct tttgcatatt gttttatttt cccttcttta aacgaggctc
    13621 agcagggtgt aaatgaaggc tgaatatacc ataaaacagg tgcaggtgta ttatctgaga
    13681 atggataata aacctgcatt tgttatttct gagggcaaag gattatctgt cgaagaattg
    13741 cctaaaccaa ttgacataga cgggtattta aagctatata catcggttgg caaggagtac
    13801 aattggattg acaggcttga aatgccaaaa gatttgttga taaatattat caataattca
    13861 aaaacccatg ttttattgtt taaagtaaac ggcacattgg ccggatgggc tgagcttgtt
    13921 gagggaagag agtatgtcga actattgtat tttgggcttt tgcccgaatt tgttggtaaa
    13981 ggtttaggaa agtcattttt ggcaatgaac attaacaagg cctgggaact taatcctgca
    14041 tggatacagc tcaacacctg cagtttagat caccccaatg ctattaatgt ttataaaagc
    14101 ttgggatttg ttgaatataa gcgtgaattt gaaatgagaa aagttttttt acgagagtga
    14161 gccaatatca gttgagtttt tcttcaagct cgagaatgat gccccccgaa tggcgggcaa
    14221 agaaattgta taaaaaagca gccacaaccc caaaaataaa gcctaatgct gccaatacaa
    14281 gcggaacaag caaaaacccg ccaattccaa gcagggggcc ccaacctgaa acatcgctga
    14341 taaattcgcc tataacaaac atgacaaaac caactatcaa tccaactata aaacctatca
    14401 aagcagacca caaggctatt actttgccaa gggagacagg tttaatccgt tttagctcat
    14461 attgaattgg ggtattctca ctcatggtta ttttgtttta ctaaggtatt ctactgtttt
    14521 tttcaatgcc tgagaaaaag gggtaacctg gtatccaagt tgcgaaacag ccttgtcaac
    14581 gctcagctcc cagttataat tgaatttacg cacatgagca ggagtaaagg caggttttag
    14641 ctttatcaaa cggttcaaca acaaaagaaa atgggcaaca aaaagcatta gccaaagcgg
    14701 gatgctaaat ttcgcaaatc tttttcctgt caatgcgtct aattgctgaa aaatatcatt
    14761 gtagctaagg ttttcaccac caaggatata tttttgaccg gctttgccat ttttcatagc
    14821 cagaatatgc cctttggcaa catcttctac ataagcataa ttgccaatac ttttaccatt
    14881 gccgggaaca aagtgccact tgccattgat atatttttta attaaaatag tcaccgaatt
    14941 gctttcgctt agtaaacccg gtccaaaaac cctggtgggg ttgacaataa caatgttcat
    15001 gcctttttga acatactggg ctataaggtt ctcagattca gcttttgata gctcatatcc
    15061 tgtaaaatga ggcaaaggcg aaatggactg ttcatcaata aattttacaa tagaaggacc
    15121 aaaaaccccg gctgtagagg tgaccacaat tttttcaacc ttagcttccc gggcagtctc
    15181 aataatatta atggttccct ggacattgta ttttttaaaa tctcccggtt tctcttccca
    15241 ggctgaggca taagccgcaa catgataaac ctgcaaacag ccttgcatag ctgttttcaa
    15301 tacatccaaa tcaaggatgt cgcctttgaa ccaagtgaca ccattgtgtt tcatcccggc
    15361 tatcttgctt tcgctgcgat aaatagcatg aacctcaaag ccttcgtcta aaagctgctt
    15421 tatcaggtgc atgccaataa aaccagttcc tccggtaaca aaaaccttta ctttttccat
    15481 aatagcttat gatatttaat ggaaaaccat tcgttgctca atgccagcgt agcatgtata
    15541 aagaaggccg cccaaatgct ccctgtttta taggttatgt aacataatac tgctcccatt
    15601 ggcaaggaac caatagtttc cttcatacct tttggcaaat gaataagtgc ataaatgctt
    15661 atgttgatta atatggcgct tttcaggtca aattcggcca tacaaaaagc aaacaaaaaa
    15721 ccccgaagca ttatctcata tcctagaaga tataataacc aagttacagc actgacaata
    15781 agtatagaag tgctaaactt ttcaatcctt atttgtgggt acatagcaag attgccatct
    15841 gtacgagcag caaaaaaatt gacaataacc ataagtactc caaaacaaag aataaaaact
    15901 agtgaaagca tagttggagc aatggcaata ccaatatatc cggccgaaaa accaatagca
    15961 taagcagaaa taaccgaagt gccgccaaag ataagcgccc caaacaggcg tacagcaaca
    16021 atatgccgcc attgtcctcc ttcattccca tatttacggg tgagcaactc aattattgct
    16081 ttgttccctt gaaaacttaa gtaagctaag aaggcaaaag ttacaatggc tactgataat
    16141 gctgtttgcg acatggttca ggcattaaaa ttcaaagtca acacaggaac gggaaagcct
    16201 tacttcacca ataaaagcac ccacaagctt atgttcaggg tgtttttgat aagtatctaa
    16261 atctttagca ttatcgaagg ttacaatcaa cacaaggtca ttgttgctgg catcaattaa
    16321 cgggtcgtta attcctacct caatagtcct tatcacatct actttccctt tgagggcaag
    16381 cagcattgac ttgatttttt caagtacttc tgttttgctc attccttcgt gttcgtcttt
    16441 aatcttccag cttacaacgt gtttaatcat aatttttcat tttatttacc aagtaacatt
    16501 tttattccaa taaaaaggag caatatggca aagccttttt gtagtgcttt ttcgggcagg
    16561 ttgacagcaa aaactgaacc aatataacta ccgagcaaga atgccagtat gactatcagg
    16621 gcaaaaggat aatttacata accgtttttg gcataattca tcacagcaaa aatacctacc
    16681 ggaaatacca atatcatcag gctggtgccc tgggcctgct gctgactaag cccaagaaaa
    16741 aaaacaagaa aagggatgat aataatagcc ccgccaaccc ccagggcccc tgataccata
    16801 ccagccagaa ggcctgtgat aacaagcaag ataacctgtt ttgctgtcaa catcataaaa
    16861 aattccattt aaacaaaatt atgatttctg tgttttatgg ctttcaaaat taagctaaat
    16921 ttgtttaaaa gatttctgta caattatttt agaaaccctt tgaaaagcta attgatgttt
    16981 gattctcaca gcaaatctaa aaaacacatt gtagtgctta ccggatcggg aattagcgct
    17041 gaaagcgggc taaagacatt tcgtgatagc ggaggcctct gggaaacata cgatgtgatg
    17101 gaagttgcca gtattgacgg gtggtacaaa aacaaagaat tggtgttgcg cttttataat
    17161 gaacgccgca aacaacttga acaagcagca cctaacaaag cccacctgtt gctcaaaaag
    17221 ttagaagata actttaaagt tagcattatt acccagaacg tagataacct gcatgaaagg
    17281 gccggaagta agaatatcct gcatttgcac ggagaactaa cgaaagtaag gagtacaaaa
    17341 gacccaaatt atattgttga tataggttat aaagaaataa aacctggcga taaatgtccg
    17401 cgtggcggcc aattgcgtcc gcatattgta tggtttggcg aggcagtacc tgccatggaa
    17461 gaggcaattg gactgacgca agaagctgat atttttgtta tcattggtac ctcgcttaat
    17521 gtttatcctg cggccggcat tgtagattat gtcaaaccag aagtgcccat tttttttatc
    17581 gaccctaagc ctgctaatac ccattatcga caaaaccttg aaattatagc cgagaaagcc
    17641 agtactggca tggaaatatt gctgaaaaag cttgaagtga gctaaacaat gcagcacaat
    17701 gcaaaccttc gggtaaacat acgatgtatt tggcaggatt ggcagcccta acgatttgcc
    17761 cccactcctg gcacaaataa ttttgttctc aaccagctta cattgtaagc ccatgaagag
    17821 ctgttataga ttttgcccaa actcatgcta ttttcattaa tccatgaagc aacaaatatc
    17881 aatcaatgct gtgctgtcaa aatctgtaaa aatcaataaa aatcgctatc tttaataaaa
    17941 actttggctg tgggagaaaa gaagcacaat gacaattttc atttatttac agtattgaaa
    18001 aaatactcaa aagaagattt tgccacccag atgaaaataa agttcatctt tctgcttctt
    18061 gtttccttta tcattttaca aacaatcatt attgtaacaa cagggtttat tcaactcaaa
    18121 acaactgaag accatgccct taaatggcca gttatcatgg ttgaagtttc actattattg
    18181 acattctgca taagtttggc ccttttaatc aaagggtttt ataaaattgt ctcacaggca
    18241 attttgatac ttacatttgt ttgtatctgg gttgtgatgt ttttggacac aagcaactct
    18301 gttacccgct tagatactgt tgtttacatt ttgcttgttt ttttggccct gcctatattt
    18361 tctttttaca aaaaatggga tatcttttta tacaccattg ccaacctgtt aacttttggc
    18421 atatttgtat atgttttttt tagtcaaaat ccgcatttat caatttatgc ctacgattac
    18481 atctccgata attcaattac cctaattatt gcaggcatcc tggcatacaa caacctgcgg
    18541 atacacagca tttcacttca aaaagccaac gaagacctta gaacccgggt tttagctgaa
    18601 gaaatgcttt tgcaaagcga aaaaaaatac cgtgaaataa cagaattgct gccacaaacc
    18661 gtttttgaag cagaactctc gggcaaactg acttatgtaa accagaacgg gttcaaacaa
    18721 tttggctata acgaagaaga cttccggcaa gggttgaatg taataaatac catacaccct
    18781 gacgacaggc agagaacgct gaataacatt caaaaaatca ttaaaggcac tcctacaaca
    18841 ggtaacttgt accgtgccat gcgcaaagat ggcactattt ttcctgttca gatttttacc
    18901 agtgtaattt accacgattc aaagcctgtt ggtttccgtg gagttattac cgatatgaca
    18961 gaacgtgtgc attttgaaga cgagatcaag aaaagccgcg accagtttca atcgctggta
    19021 gataatatac caggcattgt ttaccgatgc ctttttgaca aaaaccttac catacagtac
    19081 atgagtcccg aaactgagaa attgtgtggt tataccgcac aaaatttcat aaacaacaat
    19141 aaattttctt tttcaaacat tattcaccct gaggataacg ataaagtaat aagtactatt
    19201 gaaaaagcag taaaaaacaa aacatcatgg gaaacagagt acaggataat acacaaagac
    19261 aagcacatcc tgtgggtaaa tgaaaaaggc agggctatat acaaagacag tggccaagta
    19321 gaatatcttg atggattgat acttgacatt acccaacgca aaaatgcaga agaagcattg
    19381 cttataagcg aagagcgttt taaacaattg ttttacaatg ctccggtagc tctggccttc
    19441 atcaatgata aaggcgatat caaagggata aacaatacgt ttatcaatac gctcggctat
    19501 accatagaag atttgccatc tttagatata tggtggaaaa aagcctatcc caacaaagac
    19561 tatcgcaaag aaatagtttt tcaatggggc caaaaaacca aaagagccat tgccaatggc
    19621 caggatatat caccagatga atattatgtg acagacaaaa agggccaagg gcattatatg
    19681 ctcatagggg cctcaattca aaaaaccagc actttgttca gccttgttga cataactgag
    19741 cgcaaaaaag ccgaggaagc gttaaaacaa agcgaagaac gatttaaggc gctgatagag
    19801 tttctcccct attcagtgat ggttacagat ttacaggaac gttatgtgat ggtgaataaa
    19861 gtattttgcc aaagtatggg atattcgcca gaagaaatta taggtaaaac agcaacagaa
    19921 cttgatataa ttactaaata cgaaaatccg caagaacaga aaaaattact ttttgcacaa
    19981 ggacacatag aaaatgtaga aatggaagtg accataaaaa aaactaccag gctctatatt
    20041 tatttctctg ccaaagttat tcagatgaat aatgagccgt atctgctatg tacctcagta
    20101 aatgtcaccg aaaaaaagaa aatagaagat gaattagaaa attaccgtaa aaacctcgag
    20161 atgcttgtac aggaacgtac tgaagaactt gctactgcaa acgaagaact cgcagctgcc
    20221 aatgaagagc tttcatcaac caatgaagta cttttgcaac aacgtgaaga acttgaagaa
    20281 gcattaaaaa atctacaaaa agcccaattg caacttattg agtcagaaaa aatggcctct
    20341 ctcggggtac tggcagctgg tgtagcccac gaaattaaca atccgctcaa ctttatccag
    20401 ggaggtgttg tagctttaga aaactattta caagaaaatt accaaaacaa cacaaaagaa
    20461 accaccaccc tgttcaatgc catcaatact ggtgttgagc gtgtttcgaa gattgtaaac
    20521 agccttggcc gtttcagcag gcaaagtggg acaaacgatg agctatgcga tattcacttt
    20581 atccttgaca actgcctggt gatgctccaa aataccatta aatataaagc ctcgataaca
    20641 aaacaataca caactcaacc tttcaggctt ttggccaacg aaggaaaatt gcaccaagcg
    20701 tttttaaaca ttatctccaa tgcagctcag gcaattgaaa agaatggaga gattaccatt
    20761 accacaagcg ttgaaaataa cctgttgaca atttctgtca aagataccgg caaaggtata
    20821 aagcaagaat atcttggaaa aatattcgat ccttttttca ctaccaaaga cccaggaaaa
    20881 ggaactggtt taggcttagc tattacatac aaaatcatca aagagctggg agggaacata
    20941 agcgcagaat ctaatgaaaa cacaggaact acaatgataa ttgaattgcc ggtgagcaca
    21001 aaataaacca aaccatctta aaatcttcgt atccatttgt atctgcgcga agaatcacca
    21061 aaccataagg aaaggttcaa ttcatgcgaa cctactgtta ctttattaaa agaattgaaa
    21121 tcataatcaa aagcatagcc aacaagaaaa ttgcgaaaat acaccccggc caggaatatg
    21181 gcagtggtag ttgatcgata tgacaatcca agccagtata attctttaat aacaacttta
    21241 gcagaaaggt caatttgagg catcagcagc tcggtagatt ttgccaaagt agttaaatca
    21301 atatacgatg tattggtaaa acttttcctg taaccggcca ttaaataata atgccgctta
    21361 agattgtaat tcagtgtggt attgccaaac ctgatatttg tctgaataat gttcgatata
    21421 gaaaaaccaa aataatatga ttcaaaatct ttgtattgta tgcccacacc aatatcaggg
    21481 gcataagcag gttcttcgac aaatgcttct tttgggtcgc ccgcctcgcg aaaaacaata
    21541 ccgcttttat caattttcaa ctgcgataag ctacccgaaa ggcccaatga aagctgactg
    21601 ttggtaaaag ggatatgata agcgtatgac cctgttaaag ttgtattttg aaaataacca
    21661 tttcggtcgt ttgtaacatt taaccccacg ccaacacgcc cacttctggt agccaacatt
    21721 ttattttttt ttaatggcct ggatttaatc tgaaacttac ggagcaacaa acgggtttgg
    21781 gcacttaatg aaaaagtgcg gggggccaaa tccattccca tccattgttg tttagatata
    21841 aggctaaact ttgtgagccc aacatctcca gccaaggcag aattaaaaac aaaggcattg
    21901 tccatatatt ggctgtaaac agctgtttgt tggcttttta caagcaatgg gatgcctatt
    21961 aatagtaata tcagaaagtg ctttttaggc attctttaaa gaatctattc ttaactgata
    22021 aaatataaca ttttcagtac tacgacataa ttctaaaaat cagtcaagta caaatacaaa
    22081 agtaatgcct tttagctaat aatgattatt tgctatcgga ttattgaaac aataccaaca
    22141 atcttgggcg atccattgcc taaatcaata gagtaatagt atgaatccat tggcaggtcc
    22201 attttcatat ttgaacgtcc atcccattct tctttatatc cgcgttcaga gcgataaacc
    22261 aatgtccccc aacgattgta gatttcaaca actgccttgg gataaagctc agataatatt
    22321 tggtaatttc gaggatctcc ggccaagata ttccattttt cgttatatcc atctttattt
    22381 ggcgaaaatg ccaatgggat gctgacacaa tcattgtttt tagctttcag ctcaatagcc
    22441 atggtttggg tgcatgaatg tgcatctgta acagtaacca aatattgacc ttgttgaata
    22501 tcatacaacg catctgaagt acttccggta ttccagttga actgatatgg ggcaacgccg
    22561 ccagcagatt caactttaag ttgtccatca aaagtggcag gacaatacgg gtcagttttt
    22621 acagaaactg ttaatcgcag cgaatcaggc tgtgtcaaca caaacttccc atttttgtaa
    22681 caattattgg catcatatac atctacattg aagttacctg cacgtaaccc ttcagccagg
    22741 ctgtcccctt cgacaaaatt ccacataaaa cgatatggcc ttgtaccacc aatgacagta
    22801 gctcttgcct ttccgtcatc gcctaaatag cagtttacat tggtaaggtc aagactcatt
    22861 tgcagactat caggctgggt tattgtaata gtagagctgc cgtgacaatt ggacgagtcg
    22921 ctgacaataa cgccatattt accggctgac aagccatatt gcacagagtc gctgctgaaa
    22981 ttttgaacaa tattatccag acgtgaccaa tgatagatat acggacgagt ccctcccata
    23041 acattggcat aaagctttgc atcagaggca ttgtagcaac taatactgcg actgttgtaa
    23101 tctgactgga tgacaagttc tacattcagc ggattaggct caagtatagt aaatggaaga
    23161 ttaacaaaac atccattttt atcctgcatc acaacattat atgaaccttc aacaagatta
    23221 ttgactgaag aaacagtatc gtaagatccg tttagccatt tgtacatata tggcttaatc
    23281 cctccaaaag cattactaat aaaggcttta ccatctttta caccattgca agtctgacgg
    23341 attgaagtaa tagaagctcc cagtttatca ggttcgctca aatggtaatt ccaaacatgg
    23401 ctacaattat ttttatccct gacggtaagt ttatagttgc ctgccccaat attatcgaga
    23461 ttatttgaag tgccaattgt tacattggac gggaggtttt gccaattata agtataacca
    23521 ggcactccct gactaattgt agtatgtata aaaccatctt ttaagccaaa acacgatata
    23581 ttggaatcgt tataattgct taagaatgca ttattgctca atggcagagg ttgagtaagc
    23641 tcaaatgtat ctttttcatt acaacccttg ccatagccaa ctgtcaaaac atattgacct
    23701 gcagcaagtt tagatatact gtcacctgaa aaaggcagat tgccaatacc cactttggac
    23761 caagtgtatg aatattttcc aaagccgcca ttagccagtg ctttagcata acctgtattg
    23821 cttccattac aatcaatgcc atatccgttg taatctgatt gacgatgatc aaccacaagc
    23881 tttgctggct ttgtcaaaac aatggtatca taataatcgc aatgcatgtg catataaatt
    23941 ttacccgtat tacgtaaaga agtatcagcc aacctgaggt aatatttacc agcgtcaaga
    24001 ttagctatat cttttgttga agccgagaat gtaccagtag aatctgtcca gaaatactgg
    24061 tatgtaccat gccccccttc agggttaagc tgtatagagc catctttact gttatgacaa
    24121 gaaaccccaa aaacttcatt ttcatacggt ttagaaacaa gagggttaat tgtaactctc
    24181 gaaggttctt tcaaagtatc tgttttgggg aatgaagtac agccaagact attcttgaca
    24241 aacaatttat aaaccccctt acttagcccg gatatcgaac cttgtggccc agaataaata
    24301 acagtaaaca aacctgtatt ttcatttaat tttgaccatt cataagtata ttgaatgttt
    24361 gcatctgaag gatactggta aggggtaaat gccccatcag atttgcccgg acagctaatg
    24421 ttatattctc cataataagc cggattggtt gaaacattta accctccctg cgatgggcgg
    24481 aaaaaagcaa cagcagacgc tttacaagca ttggcatcag ttattataat cgtataatcg
    24541 ccaatagaaa gcttatcaat ggtatcattt gtactataag ggagcgtacc tcgtaaccac
    24601 agataggtgt aatctggagt ccctccgctc tgtctggcaa caatcatacc tttttcaaga
    24661 gctgtacact cagcttctga tactttaata tatgaagcta taagtttttc aggttgaatt
    24721 aaggtaattg acccatttgc caaacatcca tgagtgtcag taatatcaac ccgataatta
    24781 ccaatagtca aattctttat tgtggtatct gaagttgtta taaaattatt atcgttcttt
    24841 ttccaaacat aactggctga taaagggctc attggggtag aatatttggt catccctccg
    24901 gtcagtttaa cataaatctc acccttagca tctccaaaac aaggttcgtt ccaacctcca
    24961 acatattctt ttgcagtcag ctcagctttt aaagttggca gtatatatct ggtaaatgtt
    25021 gtgtcaactc cggcacagct aaaatctgca gggtcatttc cttttggatt attgaaaaca
    25081 ggggtaaact tgtaagttac aggctgcatt tctgttgtaa tgttggtata aatatgctgg
    25141 aaatcagcta tcggggcata aacattctgc aacccactca cctgaatttt ggctgcatca
    25201 tagcttactt caatataata atacttattc cccatcacca caccattctt acttagtaat
    25261 gtaaacaagg cagtattggt attacagaaa agcgtatcat tggaagtaac agacattcgc
    25321 ggagtggggt taacataaac ggtaatatca attgatgggc tactacatgt attatctccc
    25381 tgattattca atgcccatgg agtaattcgg tatataatat ttttaacaga agagctactg
    25441 ttatttaatg tttgaacaat tgaggagcca attgtatttc ctgtcatatc tgaattgctg
    25501 aacccggtaa tatctccagc gctattatca ataactgtcc atttatatct gataccgctt
    25561 gtagccacgc tagatgttac agg
//
LOCUS       DAOSPF010000051        25323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1965_length_25323_cov_7.532136, whole genome shotgun sequence.
ACCESSION   DAOSPF010000051 DAOSPF010000000
VERSION     DAOSPF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_25323_cov_7.532136"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..2309
                     /locus_tag="PK252_10335"
     CDS             57..2309
                     /locus_tag="PK252_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15146.1"
                     /translation="MIINNMLAAAFCTIAEKRKFIFFRKQIQRFFFLTAWFVFASSII
                     KAQTNIEELENLCKRFENENNLVELSKVQVRVGYAYQEKGNETKAVEWFQKAIKTNEA
                     LGNKNGIKNLYTNLGLIYNENNDFQKAVEFYKKSLQISQQQNKRGDILVDLINIATAE
                     QGRKNYAESNKMLEKALPLAQELNEIPSLRNIYSTLSENYDKLGQSDKAKEYFELAAS
                     IKSHLQKEELKKFESRTRQAEAEINIKEQEIESSKEQIKRISSEKELTQQLLQKEKEL
                     NELKEKDFQEKEKLQKAKQRNANIRLTLLGFILLLVSVFLLIIFRQLREKKKANLMLE
                     QSFKQIAEQKIEIEKQNKLVLQQKKKLTDSISYAQRIQQAVLPPVAAFEKVLPEHFIL
                     FKPRDIVSGDFYWVSAKEGVVIIAAADCTGHGVPGAFMSMLGVAFLNDIVNRITFNKH
                     ILALHANEILNQLRENVITSLHQTGRPDEAKDGMDIALCIVDLEHKQMQFAGAHNPAY
                     IIRKGELIVIEADHMPIGIYKTSSQPFTNHEVNLEQGDQVYIFSDGYYDQIGGPKNMK
                     MMSANFRKFLMQIHQRSMKEQKILLEDYFNQWKGDFEQVDDVLVIGFRFDHHIINTAL
                     NREYAWDDLNILIAEDIDINYFLLFEALKPTKANISRAKNGVEAVEYCKNNKVDVILM
                     DIRMPEMNGIEAAKLIREFNPKVPIIAQTANGEENDLENIKEAGCNDYITKPINLKNF
                     LATIKKHLNK"
     gene            2311..2694
                     /locus_tag="PK252_10340"
     CDS             2311..2694
                     /locus_tag="PK252_10340"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT15147.1"
                     /translation="MKSLIIEGTKNTPEINFDVKTGKFSIIGNSIPEDVLSFYTPIFR
                     WLEDYIANPCPITELSIKLSYFNSASSKTLLDILNMLEAVNDTSAKVFVKWHYHDLDE
                     DMLQTGKEFESMLSIPFEFIAYYQD"
     gene            2708..4417
                     /locus_tag="PK252_10345"
     CDS             2708..4417
                     /locus_tag="PK252_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15148.1"
                     /translation="MLKKFLFLWIVCLVHTSLFSQELPLIEKRMYTAPDGKLYIQKSL
                     PVYLMLNTNADNKGAVLLKSERTAAYTNPMYFDTEGLNTIRSPFCVDQKTKEVRKQDI
                     IFEVYADSKAPVTKISFNTGKTFKKDNKLFVKRPSQISLTANDELSGVDKIMYSLDGE
                     EFRPYVSTLEISAEGEHTLKYFAYDFVGNAEALHTLVLVVDNTKPKTSYEFKGDNFDN
                     ILAGNASIVLKSEETGSGLAGIYIRLDNGPVQKYSSAIKAATIEQGEHTLYYLASDNL
                     DNAETENTLDFYVDKTPPTIMQEIIGKTYMINGREFSSGRSQLKITGLDNKAGIKEIY
                     YSINNSEYKKYEKPVLLSSISGGLVIKAYALDNVNNRTEIRDEAQSAQLPYIDLTGPS
                     MKYSFSGPLFKYADTIYVNKNTKIHLKGVDEEAGLSQIQYNIDNLGMTNYQEPFSIDK
                     EGIHTIQYSGTDNVDNTTMGYFTVVVDNTGPEIFPRFSSLPIKSDNTQKPIYPSFAAL
                     FVAATDQTTGYEKMTYSLNGSKEIPFTGFLNAFNKNNQLTIKAFDKLGNITVLSMQFS
                     VVH"
     gene            complement(4442..5746)
                     /gene="der"
                     /locus_tag="PK252_10350"
     CDS             complement(4442..5746)
                     /gene="der"
                     /locus_tag="PK252_10350"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT15149.1"
                     /translation="MSNIVAIVGRPNTGKSTLFNRLTESRQAIVDETAGTTRDRHYGK
                     AEWNGVEFSVIDTGGYVNDSDDIFEEEINKQVELAIEEADVILFMVDVITGVSDLDKA
                     VAAILRRSGKKVFLTANKVDNYGLVSQSYEFYSLGLGDIYCISAMSGSGTGDLLDAIV
                     GSFTKETKDEAIDLPKFTIVGKPNVGKSSLINALIGQDRSIVTPIAGTTRDSIFVRYN
                     KFNHDFYIVDTAGLRKKNKVKEDIEFYSVMRSVRAIEQADVCLLLIDATIGIEAQDMS
                     IFQLCQRNKKGLVIVVNKWDLVEKDNHTTEQYKQVIYNRIAPFTDVPIVFASAVTKQR
                     IHKILETAIEVYQNRTQRISTSKLNEVMLAAIDAYGPPAVKGKLVKIKYVTQLPTPVP
                     SFVFFANLPQYIRDPYKRYLENKLRENFNFSGVPIQIFIRQK"
     gene            complement(5791..6663)
                     /gene="era"
                     /locus_tag="PK252_10355"
     CDS             complement(5791..6663)
                     /gene="era"
                     /locus_tag="PK252_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT15150.1"
                     /translation="MHKAGFVNIIGKPNVGKSTLMNAMIGERLSITTPKAQTTRHRIM
                     GIANGEDYQVVFSDTPGIIKPKYKLQEKMMNFVESSFYDADIMLYLTDVEEKFDNHEL
                     IEKLNKISVPLIVAINKIDISDQSKTLVLLAEWQKLLPKAEIFVISALHKHNIDGLFK
                     RLLELLPENPPYYSKEDLSDRNLRFFVSEIIREQILKNYSKEIPYSVEVAIESYKEAK
                     DCDRIAAVIYVERDSQKGIIIGAKGESLKRVGVQSRIQIEKLIGKKVFLEIFVKVKKD
                     WRSDDSILKSFGYQ"
     gene            6900..7940
                     /locus_tag="PK252_10360"
     CDS             6900..7940
                     /locus_tag="PK252_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT15151.1"
                     /translation="MVIDEILVLMLEKQGRVSIPNIGKIEITYSAANIDETGNKIKPP
                     SSNFHFENVKADFLKDEFIKSIKQIQPSLSQPDIDFHIKECITIADNAILGKGYFFKN
                     IGKISYESGNFIFVPDKDSFLLQDFYGLEDVKVPESFKSEPKTTEAPSVSNKETVTPK
                     VVNLPPPVPHQTKKPQAQKTGIPKYVKIAIAISAIFIVVSITYTYWGKISSKKGLFGF
                     FNTTSDKKQEKEKTYAYTDTLENKIDAEALKRKALDYSEDTIQKSPIAPNARMTYYII
                     AGSFKTSNNAEKLVTDLIKKGFEPKIISLGDTLFRVSLKKFKVRTDAIDEYVMISKTH
                     PDLKIWVYSRIE"
     gene            7985..8956
                     /gene="rfaD"
                     /locus_tag="PK252_10365"
     CDS             7985..8956
                     /gene="rfaD"
                     /locus_tag="PK252_10365"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOT15152.1"
                     /translation="MQKKLMIVVTGAAGFIASNVVKLLNENNFNDVVIVDDFSKTEKK
                     YNYEGLRFTHQVNRDEFFGWVKANHRFIQIIIHLGARTDTTEFDKNIFDKLNLNYSKE
                     VWNLCTEYGLPLIYASSAATYGNGELGYDDNHAIIPQLKPLNPYGDSKNEFDKWALKQ
                     EKQPYFWAGLKFFNVYGPGENHKGRMASVIYHAYNQILKTGSMKLFRSHRPDYKDGEQ
                     LRDFIYVKDVADVILFLMQHRKNSGIYNLGTGKARTFIDLVKATFKAMNIPEKIDFID
                     TPADIRDKYQYYTQANMDKLRSIGYVKPFTSLEDGVEEYVKKYLMAK"
     gene            9655..10122
                     /gene="mraZ"
                     /locus_tag="PK252_10370"
     CDS             9655..10122
                     /gene="mraZ"
                     /locus_tag="PK252_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT15153.1"
                     /translation="MASFIGNYTCKTDEKGRVTLPTAFKKALPAEAHDTFIVKKDIYQ
                     KCLVLYPQNEWEKQVEQIRASLNPYNKEHMIFLRGFYKDTAEVELDATNRLLIPRRLL
                     DEAGIGKEAVLAGIDNKIEIWSKESYDTIDAGEDFAILAGKILGGTNQKTEKP"
     gene            10119..11015
                     /gene="rsmH"
                     /locus_tag="PK252_10375"
     CDS             10119..11015
                     /gene="rsmH"
                     /locus_tag="PK252_10375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT15154.1"
                     /translation="MTYHIPVMLHESIEGLRIKPSGCYVDATFGGGGHSKAILAQLKG
                     GRLFGFDRDEDAMQNIPSDPQFTFVKQNFRYMKNFLNYHGVEKIDGILADLGVSSHQF
                     DEPQRGFMFRADAPLDMRMDKNSNLSAKTLLNEWTEEKLADLFYLYGEIQNARKLAHC
                     ITTARSRQAISTTGELVECIRSCIPQKIENKYMAQVFQAIRIEVNGELDNLKQLLEQS
                     AEMLVPSGRLVVITYHSLEDRMVKNFIKCGNVEGNENKDFYGNRNAPFKAINKSVITP
                     SDEELKTNNRSRSAKLRIAEKL"
     gene            11026..11409
                     /locus_tag="PK252_10380"
     CDS             11026..11409
                     /locus_tag="PK252_10380"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOT15155.1"
                     /translation="MSEEKEKKNIEFVSEKEEVKEIQKYSVKHWITGRVFASAEIRSQ
                     LPFIVFLAFLGFVYIANRYHAERLMRNTLKLKQELDELRAESITTASELMFLSRQSQV
                     QKFVNQENLGLKEAVAPPKRIKWRD"
     gene            11417..13531
                     /locus_tag="PK252_10385"
     CDS             11417..13531
                     /locus_tag="PK252_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOT15156.1"
                     /translation="MSIKKDIVWRIALVYVVMLLVGIAIVMRIVYLQFFEVGPLTKKA
                     QKLSVRDIEIIPERGNILACDGRLLATSVPYYEIRVDISRETVPSGLFIEKIDSLATR
                     LAQIFKDKSWGMYKRELIHARNENNRYYLLKRKADFEQLHQLRNCPIFRLGKNKGGLI
                     VIPNGVRFMPHGDLAARTVGYISKSEEGTVVGIEGSYDEELRGVKGIKLMQRVPGGIW
                     MPINSENEIEPKNGVDVVTTIDLNIQDVAQEALRTQLQKHNAHHGTAVLMEVKTGEVK
                     AIANLEKTENGQYREMFNYAIAESTEPGSTFKLATIMAALEDGYITPDDTIDTQNGTW
                     KIYDKEIKDSHEGGYGKISINQVFELSSNIGVAKIVMDNYKGREEQFIKRIYSFGLND
                     KLGLQIKGEGSPMIKYPHTQGWSGISMAMISHGYELRLTPLQVLAFYNAVANNGRLVR
                     PQFVKELRVNGRTIKRFENHTIKSSICSMSTIRKVRKMMEGVVLRGTATNLKDSVLHI
                     AGKTGTAQIARKNQGYRQNSQIAYQASFVGYFPAEAPKYSCIVVVNAPSNSVYYGNLV
                     AGPVFKEIAQKVYATSFELHDEIIGGRNNLKTELPYAYDGNREQIEEVLDELGIDYDE
                     NDESQWISLYKEDDEFKLKKRTVIPNLVPNVTGMPAKDAVYLVEKAGLHPIVKGYGKV
                     FSQSIGPGSKASSGSSIILQLR"
     gene            13543..15006
                     /locus_tag="PK252_10390"
     CDS             13543..15006
                     /locus_tag="PK252_10390"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT15157.1"
                     /translation="MKTLKDILKSVEAKSIKGSIDIAIEGIQFDSRKVVKNTVFVAVP
                     GTQNDGHQYIEKAIELGATAIICQTLPKSLNTNITYIEVADSAWVLGIAIANFYDNPS
                     SKLKLVGVTGTNGKTTTATMLYWLYKAMGYKVGLISTVIYYVNDEAFESTHTTPDPLM
                     LHSLMAKMADAGCQYCFMEVSSHSIAQKRIAGLHFDGAIFTNLTHDHLDYHLTFDNYL
                     KAKKELFDSLPKEAFAIVNIDDKNGRVMVQNTKAEVKTYALQSVADFQAKIIESHFDG
                     MQLIFDGKDFWTPLTGKFNAYNLLAIYSTAVMLGSNKEEVMTKLSTFGHVRGRFETIR
                     AKNGMTAIIDYAHTPDALENVLNAIRQIRQSHQKIITVVGAGGNRDKTKRPVMAKVCV
                     TLSDKVIITSDNPRFEEPEDIIADMMQGIDAKDKLKTLKITDRREAIKTAVMLATPDD
                     IILIAGKGHETYQEIKGVKHHFDDKEEIAQLLMTNDK"
     gene            15003..15215
                     /locus_tag="PK252_10395"
     CDS             15003..15215
                     /locus_tag="PK252_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15158.1"
                     /translation="MIENKENIIVSNPFQLALSFIEFCQKFENTAKLAIVKQLLKVAA
                     LIRVKIHEKLLAQNKNNKNYSLAISN"
     gene            15223..16458
                     /gene="mraY"
                     /locus_tag="PK252_10400"
     CDS             15223..16458
                     /gene="mraY"
                     /locus_tag="PK252_10400"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT15159.1"
                     /translation="MLYHLFTYIDKVNHIPGTGLFNFISFRASMAALLSLAIALLIGK
                     KIIYFLQRKQIGEEIRDLGLEGQMQKKGTPTMGGIIIIAAIVIPVLLLADLTNIYTIL
                     LLFTTLWLGMIGFIDDYIKVYKKNKEGLHGKFKIAGQIILGLVVGLTLWLHQDVVIRD
                     KKASHKQKMENVQTTDQTIAKKVDIKSTATTIPFVKNHEFNYNKLLWFMGENARKFQW
                     IIYILVIIVIITAVSNGANITDGLDGLATGTSAIIGSVLGILAYVSGNIIYADYLNIM
                     YIPHIGELMIFAAAFMGATIGFLYYNSYPAQVFMGDTGSLTLGGFIAVFAIVIRKELL
                     IPILCGIFLAENLSVMVQVSYFKYTKRKYGEGKRILLMAPLHHHFQKKNYHEAKIVTR
                     FWIIGILLAIITLATIKIR"
     gene            16478..17812
                     /gene="murD"
                     /locus_tag="PK252_10405"
     CDS             16478..17812
                     /gene="murD"
                     /locus_tag="PK252_10405"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT15160.1"
                     /translation="MKRIVILGAAESGVGAAVLAKAKGFDVFVSDAGKIKESYKNMLI
                     GHQIDFEEGKHSEDLILNAHEIIKSPGIPEKAEMVKKIRTKQIPVISELEFAARYTNA
                     YKICITGSNGKTTTTSLIFHMLKKAGLNVGLAGNIGQSFAYQVATQDFDYYVLEISNF
                     QLDDMYEFKADIAILLNITPDHLDRYDYDLQRYIDSKFRITQNLTDEEYFIYCSDDEL
                     TAREMEKIVTKAKQLPFSLKEKKLPGAYVDNNFIHIGLDDVDFSMSIDELSLKGKHNT
                     YNSMAAGIAGNALRIHNEIIREALSDFKGVEHRLEMVCKVNDISFINDSKATNVNSVW
                     YALECMTTPVIWIAGGTDKGNDYSELFELVKSKVKALVCLGVDNSKLHKAFDDKIKII
                     QDASSMQEAVRKAFHLASAGETVLLSPACASFDLFENYEDRGRQFKKYVKEL"
     gene            17829..19088
                     /locus_tag="PK252_10410"
     CDS             17829..19088
                     /locus_tag="PK252_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT15161.1"
                     /translation="MFERVEKNLKGDKIIWAIILLLSLLSLLAVYSSTGTLAYRYHGG
                     NTIYYFFKHASYMLLGLGAIYITHLIPYKYYLRLAQIFLYLSAPLLLLTLVMGTSLNS
                     ASRWLTVPVIGLTIQTSDFAKIALIMYVARTLALKQDEIKDFKKAFLPVIIPVGIICT
                     LILPANLSTALMIFMVSVILMFIGRVSIKHLSLLGLCGIIFLSLFIIIAPMTHTNLGN
                     RVKTWKHRIVNFSGNNDDKKETNDANFQSEQAKIAVATGGILGKGPGNSTQRNFLPHP
                     YSDFIYAIIVEEYGLVGGIFVLSLYLALLFRAGVIVRKSERTFPAFLAIGLALLLVFQ
                     AMINMAVSVNLVPVTGQTLPLVSMGGSSMLFTSAAIGIILSVSRSIIKEKEVKEENKE
                     DENKEKKEDENNINTQAKEKVQEKETE"
     gene            19126..20253
                     /gene="murG"
                     /locus_tag="PK252_10415"
     CDS             19126..20253
                     /gene="murG"
                     /locus_tag="PK252_10415"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOT15162.1"
                     /translation="MQKNREIRVIISGGGTGGHIFPAIAIANAIKAEKTNAEILFVGA
                     LGRMEMQKVPAAGYNIVGLPIMGFNRSSLWKNFEVLAKLVKSLFKARKIIKDFKPDMA
                     IGVGGYASGPLLWMASFSKVPIFIQEQNSYAGVTNKLLAKRAKKIFVAYPDMQKFFPA
                     NRIIISGNPVRGGLNNVSNLKHEAIDFFKLDPAKKTILCIGGSLGARTLNESIINNIN
                     KIVESNYQLIWQCGAYYHAACQKSIENLKNQNIKLYDFINRMDLAYAAADVVISRAGA
                     LSISELCLVSKPVILVPSPNVAEDHQTKNALALTEKNAAVMITDNDAPKKLIDETFAL
                     LNNENKSQMLANNISKFAMHDAAKNIAREIIKNVKPEMTNQ"
     gene            20250..21638
                     /gene="murC"
                     /locus_tag="PK252_10420"
     CDS             20250..21638
                     /gene="murC"
                     /locus_tag="PK252_10420"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT15163.1"
                     /translation="MTFKIENIKNVYLVGIGGIGMSALARYFKTIGRNVAGYDKTPTP
                     LTSKLTEEGIDIHFDDNLALVPEYIKKEKDNTLVIFTPAVPSWHVELNFFKNNGFTIM
                     KRAEVLGHIFSQGIGAAVAGTHGKTTTSTMLAHILHNSQKQCNAFLGGIARNYNSNLL
                     LSDKSQIVVAEADEYDRSFLRLYPTVAIVTSVDPDHLDIYGSYSEVIKSFNDFVNQIK
                     PGGALVYKKNIAIDLNKLEKINKYSYDINNDADFSARNLRIESRGFCFDLYTPERNLE
                     GIQLRIGGAMNVLNATAAAAAAHLLGVGYDEIIAGLESFEGVKRRFEIHLNENGRTYV
                     DDYAHHPEELKACIKSARQIFPNRKLCGVFQPHLYSRTRDFADEFAKSLDMLDEIILL
                     NIYPAREEPIPGITSHVIFEKMKNANKVLCSKDQLIKELKNRDIDVLLTLGAGDVDQM
                     AADICNIIKQQA"
     gene            21635..22426
                     /locus_tag="PK252_10425"
     CDS             21635..22426
                     /locus_tag="PK252_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15164.1"
                     /translation="MKKAINISLWVVLIAYLGAIYGFVEEKNKGIICNDINVSVVNNE
                     TNKLISNKSILATIKNSHIPIIGKQVNKINTFALENLFKSNATIKQVAIYTTIDGILN
                     VEAEQREPILRVMNARMQQYYIDNEAYIIPIRFEAPAYCLFANGNIKETFNVNEAHTE
                     KSLLMDTLNNNKNNISSIFKLSQYINSHNFWKNHISQIYVDGDNNLMVIPRVGAHVII
                     LGDITNLDIKFKKLRSLYYAFNEIGWNDYKTINLKYSNQIVCTKR"
     gene            22431..23801
                     /gene="ftsA"
                     /locus_tag="PK252_10430"
     CDS             22431..23801
                     /gene="ftsA"
                     /locus_tag="PK252_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT15165.1"
                     /translation="MIHKEQIIGAVDIGTTKIVTIIGKINANQRLEVLGLSCTESRGV
                     KRGVVQNIEETVMSIKHTIKEAEAQAGYKLNEVFVGIAGQHIHSIKSSGYLLRDSYDN
                     EITAEDLKRLENDMRKIPVDVGEEIIHVIPQSYTVDNEPDIKNPVGMFGKRIDANFHI
                     VIASVTFANNIKRCIEKSGLQVKSLILEPLASSEAVLTEDEKEVGVAMIDIGGGTSDL
                     AVYYENIIRHTAVIPLGGNIISKDLKDGLCILPRHAEMLKIKHGSALQALAQPNDFVS
                     VPGISGREPREISTATIAGIIQCRVEEILNFIALEIERSGFQDKLSAGIVVTGGGSML
                     KNIRQLINFRTGMDSRIGFPTEHLVASSVADVNHPKYATAIGLVFKGYQYLNEHKEVM
                     SFTNIGEEITAGFQPNTAPTSENKTAVKEEEFKEPENKDITKSNFLNSLLYKFTVMFD
                     ENDTKM"
     gene            23832..25250
                     /gene="ftsZ"
                     /locus_tag="PK252_10435"
     CDS             23832..25250
                     /gene="ftsZ"
                     /locus_tag="PK252_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT15166.1"
                     /translation="MEDLINFDIQANKSSIIKVIGVGGGGSNAVNHMFRMGIKDVDFI
                     VCNTDAQALANSPVPIKIQLGNSLTEGRGAGNKPDVGRQSAIESLPEIMEALSMNTKM
                     AFITAGMGGGTGTGAAPVIAKAVKEQGILTVGIVTIPFRFEGPVRINQAIEGINELRN
                     YVDSLLVIHNEKLRELYGNLKISEAFSKADNILTVAAKGIAEIITVHGYVNVDFADVQ
                     TIMANSGVAIMGSATASGQDRALEAIQQALTSPLLNNNDIRGAKSILLNITSGEDEIS
                     MDEVGIITDFVVNCVDKDALMIWGTGIDPSLDDKISVTIIATGFPDNSIPELITRKAQ
                     PAKVAATINEPFTSKLPFEVKHSKENFQPIQKTIEFDFGSSALDVPLVESPINQSKAN
                     ERLSEIKKQSVKSDGILPNHLDPNVIEELENTPAFMRKNIKLKESKSASGPEISRLTL
                     FDSDEDIIKISAENPYLNKNVD"
BASE COUNT         8663 a         4966 c         4771 g         6923 t
ORIGIN      
        1 aatgcttcgc ctaaaggcga gggatttcac ccatcccaga attataaatt aaaaagatga
       61 taatcaacaa catgttagct gctgcgtttt gtaccattgc tgagaaaaga aaatttattt
      121 ttttcaggaa acaaattcaa aggtttttct ttttgacagc ctggtttgta tttgcttcat
      181 caataattaa agctcaaaca aacattgaag aattggaaaa tttatgcaaa cgctttgaaa
      241 atgagaataa tttggttgag ctcagtaagg tacaagtaag agttggctat gcataccagg
      301 aaaaaggcaa tgaaacaaag gctgttgaat ggtttcagaa agcaataaaa accaacgagg
      361 cacttggaaa taaaaatgga ataaaaaact tatataccaa tcttggactt atttataatg
      421 aaaataatga ttttcagaaa gcggtagaat tttataaaaa gtcacttcaa atcagccaac
      481 agcaaaacaa gcgtggagac atcctggttg acctaatcaa tattgctacc gcagagcagg
      541 gacgaaaaaa ttatgccgaa tcaaacaaaa tgttggaaaa ggcgttacca ttggcccaag
      601 agcttaacga gataccatca ttgagaaata tttacagcac cttatctgaa aactatgata
      661 aactcggcca atcagataaa gctaaggaat attttgagct ggcagcttct attaaaagcc
      721 atttgcaaaa agaagagttg aaaaagtttg aaagccgtac cagacaagct gaagcagaaa
      781 tcaatattaa agaacaggaa atagaaagca gtaaagaaca aataaaacgc attagcagcg
      841 aaaaagagct tacccagcaa ttgctccaaa aagaaaaaga gctaaacgaa ttgaaggaaa
      901 aagattttca agaaaaagaa aaactccaaa aagctaaaca acgaaatgct aacatcaggc
      961 ttactttgct ggggtttata ttgcttttgg tttctgtttt tcttttgatt attttcaggc
     1021 aattgagaga gaagaaaaag gccaacctga tgttggagca aagttttaag caaatagccg
     1081 aacaaaaaat tgaaatagaa aaacaaaaca aactcgtttt gcaacaaaag aaaaagctta
     1141 cagacagcat ttcgtatgca caacgcatac aacaagcggt actcccgcct gttgcagctt
     1201 ttgaaaaagt attgcccgaa catttcatat tattcaaacc acgcgacata gtgagcggcg
     1261 atttttattg ggtttcggcc aaagagggcg tagtgattat tgcagctgca gattgtacag
     1321 ggcatggggt acccggagct tttatgagca tgcttggcgt agcattttta aatgatatag
     1381 taaaccgaat tactttcaac aagcatattt tggcccttca tgctaatgaa atacttaacc
     1441 agcttagaga aaatgtaata acctcgttac atcaaaccgg ccggccagat gaagcaaaag
     1501 atgggatgga catagccctt tgtattgttg atttagagca taagcagatg caatttgccg
     1561 gggcacacaa tccggcatat atcattcgta agggagaact tatagttata gaagcagacc
     1621 acatgcctat cgggatttac aaaacatctt cgcagccttt taccaatcac gaggtgaact
     1681 tagaacaggg tgaccaggtg tacatttttt cggacggata ttacgaccag attggtggcc
     1741 ctaagaatat gaaaatgatg agcgccaact ttcgtaaatt tttgatgcaa atccatcaaa
     1801 gatcaatgaa agaacagaaa atactgcttg aagattactt taaccaatgg aagggagact
     1861 ttgaacaagt tgacgatgta ttggtgatag gctttaggtt tgaccatcac attattaaca
     1921 ctgcattgaa cagggaatac gcgtgggacg acttgaacat actcatagca gaagatattg
     1981 atatcaatta tttccttttg tttgaagctt taaaaccaac taaagcaaat atatcacgtg
     2041 caaaaaatgg ggttgaagca gttgaatatt gcaaaaacaa taaagttgat gtaatattga
     2101 tggatatccg catgcccgaa atgaatggca ttgaagcggc aaaactaatc agggaattta
     2161 accctaaagt gcctatcatt gcccaaacag ccaatggtga agaaaatgac cttgaaaaca
     2221 ttaaagaagc cgggtgtaac gactacataa caaaacccat caacctgaag aattttttag
     2281 ctacaattaa gaaacattta aacaaataat atgaaatcgt taataattga agggactaag
     2341 aatacgcccg aaatcaattt tgatgtaaaa actggcaaat tttccatcat tggcaattca
     2401 atccccgaag acgtgcttag tttctatacg ccaatattca ggtggcttga agactatatt
     2461 gccaatcctt gccctataac agagctctcg ataaaattga gctattttaa ctctgcttct
     2521 tcaaaaacct tacttgacat cctcaacatg cttgaggctg tcaatgacac ctctgccaaa
     2581 gtatttgtaa aatggcatta ccacgattta gatgaagata tgttgcaaac aggtaaagaa
     2641 tttgagagca tgttaagtat cccttttgaa tttattgcgt attatcaaga ttaataaaag
     2701 atttattatg ttaaaaaaat ttctttttct ttggatagtt tgtctggttc acacaagtct
     2761 gttttcgcaa gagcttcctt tgattgagaa gcgaatgtac acagctccgg atggcaagct
     2821 gtacatacaa aaatcgttgc cggtatatct tatgctcaac accaatgccg acaataaagg
     2881 ggctgtattg ctaaaaagtg aacgtactgc agcttatacc aacccaatgt atttcgacac
     2941 agaaggttta aacactattc gtagcccttt ttgcgttgac caaaaaacaa aagaggttcg
     3001 aaagcaagat ataatttttg aagtttatgc agacagcaag gcccctgtaa caaaaatttc
     3061 ttttaacacc ggaaaaacct tcaaaaaaga caataagctt tttgtcaaaa ggccttcaca
     3121 aatttcgtta acagcaaacg atgaattaag tggagtggat aaaattatgt attcattgga
     3181 tggtgaagag tttaggcctt atgtttcaac ccttgaaata tctgccgaag gtgaacacac
     3241 actaaaatat tttgcgtacg actttgttgg caatgcagag gctttacata cgcttgtttt
     3301 ggttgttgac aatacaaaac ctaagacaag ctatgaattt aaaggagata attttgacaa
     3361 tatccttgcc ggcaatgcaa gtatagtcct caaatctgaa gaaactggca gtggtttggc
     3421 cgggatttac atccgccttg ataatggccc tgtacaaaag tattcttctg caataaaggc
     3481 tgctacgata gaacagggag agcatacgtt gtattacttg gcatctgaca atcttgacaa
     3541 tgcagagact gaaaacaccc ttgatttcta tgttgacaaa acacctccca ctatcatgca
     3601 ggagattatt ggaaaaacct acatgataaa cggaagagag ttttcttcag ggcgttcgca
     3661 gttgaaaatt accggacttg acaataaagc cgggataaaa gagatatact attctatcaa
     3721 taacagcgaa tataagaaat atgagaagcc tgtattgctt agttcaataa gcggaggact
     3781 tgtaataaaa gcttatgccc tggacaacgt aaataaccgt accgaaatca gggatgaagc
     3841 ccaatcagcc cagttgcctt atattgacct taccgggcct tcaatgaaat attcattttc
     3901 agggccacta tttaagtatg ccgacactat ttatgtgaat aaaaatacca aaatacatct
     3961 gaaaggggtt gatgaagaag ccggacttag tcaaattcaa tacaatatag ataatttggg
     4021 catgactaac taccaagagc cttttagtat tgacaaagaa gggatacaca ctattcagta
     4081 ttctggcaca gacaacgttg acaacaccac catgggctat tttactgtag tagttgacaa
     4141 tacaggcccg gagattttcc ctcgctttag ctcattaccc ataaaatcag acaacacaca
     4201 aaaacccatt tatccatcat ttgcagccct atttgttgca gctacagacc aaactaccgg
     4261 ttacgaaaaa atgacttatt cgctcaatgg aagcaaagaa atacctttta caggtttttt
     4321 aaacgccttt aacaaaaaca accaattgac tatcaaagct tttgacaaac ttggcaatat
     4381 aactgtactc tcaatgcagt tttcggttgt acattaagat tatcgcccga taaatcttca
     4441 tctacttttg ccttataaat atttgaattg ggaccccgct aaaattaaaa ttttcacgca
     4501 atttattctc aagatacctc ttgtatgggt cgcggatgta ttggggtaaa ttggcaaaaa
     4561 atacaaatga cggaacaggg gttggcaatt gtgtaacgta tttaatctta accaattttc
     4621 ctttaacagc aggcggccca taagcatcta tcgctgcaag cataacctca ttgagctttg
     4681 aagttgaaat acgttgtgtc ctgttttgat aaacctcaat agctgtttct aatattttgt
     4741 gaatacgttg cttggtaaca gctgacgcaa aaacaatggg gacatctgta aatggggcaa
     4801 tacggttata gataacctgc ttgtactgct cagtagtgtg attgtctttt tctaccaaat
     4861 cccatttgtt gaccacaata accaaacctt ttttattgcg ctgacatagt tggaaaatgc
     4921 tcatatcttg agcttcaatg ccaatagttg catcaataag caaaagacac acgtctgctt
     4981 gttcaatagc ccttacggag cgcattacag aataaaactc aatatcttct tttactttgt
     5041 ttttcttacg gaggccggca gtatcaacaa tataaaagtc gtgattaaac ttattgtacc
     5101 ttacaaatat tgaatcacgt gtagtaccag cgataggggt tactatgctt ctatcttgtc
     5161 ctattaacgc attaataagt gatgatttac caacgtttgg ttttccaacg atagtaaatt
     5221 tgggcaagtc aatagcttca tccttagttt ctttagtaaa tgaaccaaca atggcatcaa
     5281 gcaagtcgcc tgttccactg ccagacatag ctgaaataca ataaatatca cctaacccga
     5341 gagaataaaa ctcatacgac tggctaacca gtccgtaatt atcaacttta ttagcggtaa
     5401 gaaacacctt tttaccagac cttctaagaa tagcagctac agctttgtca aggtctgaaa
     5461 ccccggtaat aacatcaacc ataaaaagga taacgtctgc ttcttcaatg gccagctcta
     5521 cttgtttatt tatctcttct tcaaaaatat cgtcagagtc attgacgtat ccgccagtat
     5581 caataacaga aaactcaacg ccgttccatt cagctttccc gtaatggcga tcgcgtgtag
     5641 tacctgctgt ttcgtcaaca atagcctgcc gactttcggt aagcctgtta aacaatgtcg
     5701 atttgccagt atttgggcgg cctacaatgg ctacaatatt gctcatatta cgatgtttta
     5761 aaaaagggct gcaaagatac tcgaattatt ttactgataa ccaaaacttt tcaatatgct
     5821 gtcatcgcta cgccaatcct tttttacctt gacaaatatt tccaaaaaaa cttttttacc
     5881 aataagtttt tcaatctgaa ttctcgactg tacgcctaca cgtttaagcg actctccttt
     5941 tgctccgata ataatacctt tttgcgaatc acgctcaaca taaataacag cagcaatgcg
     6001 gtcgcagtct ttagcttctt tatacgactc aatggcaacc tcaaccgaat aaggtatttc
     6061 tttagaatag tttttcagta tctgctcacg gataatttcc gaaacaaaaa agcgcaggtt
     6121 gcggtctgat aagtcttctt tggaataata aggcggattt tccggcaaca gctcaagcag
     6181 tcttttaaaa agaccatcaa tattatgttt gtgaagcgct gatatgacaa aaatttcggc
     6241 tttaggcaaa agtttttgcc attcggccag caaaaccaat gtttttgatt gatctgaaat
     6301 gtcaatttta tttatagcca ctattaacgg cacagaaatt ttgttgagtt tttcaatcaa
     6361 ttcatgattg tcaaactttt cttcaacatc agtaaggtaa agcattatat cggcatcata
     6421 aaacgaagac tcaacaaaat tcatcatttt ttcctgtagc ttgtatttag gctttatgat
     6481 gccgggggta tccgaaaaaa ccacctgata atcttcacca ttggcaatac ccatgatacg
     6541 gtgccttgtt gtttgagctt tcggagtagt aattgaaagc ctctcaccaa tcatggcatt
     6601 cattagtgtt gattttccca cattaggttt accaataata tttacaaaac cagctttgtg
     6661 cattttttat gcaaaggtag gaaattaatg gttaatggtt aatggttaaa actaattacc
     6721 aatgaccaaa ttataaaaca atatcgtttt gtcaagtaaa aagctatgat gcataaaaaa
     6781 tcgcatgttt atttccaaat cagttctgac atcagaagct acgcattgtt tttttaattc
     6841 atatttgaca tttgatattt ttgccaaaaa acatgtatgt ttgttcaaaa atcagcaaca
     6901 tggttattga tgaaatattg gttttaatgc tcgaaaaaca gggaagagtt tcaatcccta
     6961 acattgggaa aattgaaatt acctactctg cagcaaacat agacgaaaca ggcaacaaaa
     7021 tcaaaccacc ttcttcaaac tttcatttcg agaatgttaa agctgatttt ttgaaagacg
     7081 aatttataaa aagcataaaa caaatacaac cttcattaag ccagcctgat attgactttc
     7141 acattaaaga atgtattact attgcagaca atgcgatact cggcaaagga tactttttta
     7201 aaaacattgg aaaaataagc tacgaaagtg gcaatttcat ctttgtccct gacaaagaca
     7261 gctttttatt gcaggatttt tatgggcttg aagatgtaaa ggtacccgaa tctttcaaat
     7321 cagaaccaaa aacaactgaa gccccttcag tttcgaataa agaaacggtt acgcctaagg
     7381 ttgtcaacct gccccctcct gtacctcatc aaactaaaaa accgcaagca caaaaaacag
     7441 gaatacctaa atatgtaaaa atagctattg ccatttcggc tatttttata gttgttagca
     7501 tcacttacac ttattggggc aagattagtt caaaaaaagg gctgtttggc tttttcaaca
     7561 ccacatctga caagaagcag gaaaaagaaa aaacctatgc ttataccgat acccttgaaa
     7621 ataaaattga cgcagaagcg ctaaaacgaa aagccttaga ttactcggaa gacacaatac
     7681 aaaaaagccc tatagcccca aatgcccgta tgacgtacta cataattgcc gggagcttca
     7741 aaaccagcaa taatgccgaa aagcttgtaa cagaccttat aaaaaaagga tttgaaccaa
     7801 aaattatttc gttgggagat acacttttcc gcgtttcttt aaaaaaattc aaagtgcgaa
     7861 ctgacgctat tgacgaatat gttatgattt caaaaaccca tccagatttg aaaatatggg
     7921 tttatagccg aatcgaataa aatcaactgg ttaagatttt gccagtgaca taaaatatct
     7981 aactttgcaa aaaaaactta tgatagttgt taccggagct gctggtttta ttgcaagcaa
     8041 tgttgtaaaa ctcctcaatg aaaataactt taatgacgtt gtcattgttg acgatttttc
     8101 taaaacagaa aaaaaataca attacgaggg cctcaggttt acccatcagg taaatagaga
     8161 tgagtttttc ggctgggtaa aagcaaacca ccgctttata caaattatca tccaccttgg
     8221 cgcccgcacc gacactaccg aatttgacaa aaacattttt gacaagctca acctcaatta
     8281 cagcaaagaa gtgtggaacc tttgcaccga atatggtttg cccctgattt atgcttcatc
     8341 tgctgctact tacggtaatg gagaattagg atatgatgac aaccatgcca ttataccgca
     8401 acttaaaccg cttaaccctt atggcgattc gaaaaatgaa ttcgacaaat gggctttaaa
     8461 acaagagaaa caaccatact tttgggcagg gctcaagttt tttaatgtat atgggccagg
     8521 cgagaaccac aaaggacgca tggcttctgt tatttatcat gcctataacc aaatattgaa
     8581 aaccggcagc atgaagcttt tccgttcgca ccgtcctgac tacaaagatg gcgaacaact
     8641 ccgtgacttt atttatgtaa aagatgtggc cgatgttatt ttatttttga tgcaacatcg
     8701 caaaaatagt ggcatttaca atctcggtac cggaaaagcc cgtacattta tcgatttggt
     8761 aaaggctacc tttaaagcta tgaacatccc tgaaaagatt gattttattg acacccctgc
     8821 cgatattcgc gacaaatacc aatactacac tcaggccaac atggacaaat tgcggagtat
     8881 tggatatgtc aaacctttca cttcacttga agatggtgta gaagaatatg taaagaaata
     8941 tctcatggca aaataaagcc ggccattgac ttgttgcctc ttaaagtatt tgaatttatt
     9001 tttcaatctt tagctgtaag ccatttgccc ctaaaaatta caaactcccc ctgacggagt
     9061 aaaatttgac acgtcttatt tttcgctatt ttcagcaatg atattttctt ctctcgttag
     9121 cccataagcc aaacagtcga tttgggtctc taaattagac ttaataaaat cattctttca
     9181 atttagaaat actataatat cgttgatatg aaactgatcg ccccgccggg gcttaatttt
     9241 aacgatgttt aattttacta caaacaggac gtcgctacgc gactaaataa tagcagcgta
     9301 gctgcaaact gtttgaagga tgaccaaata caccaaatgt cctaagtctc caataaagca
     9361 ttatttaacc caaatacgac accccttttt ttacaacttt aagaacactt gtaaatataa
     9421 aatatgcaga cattcttctt ttgtcaaaca gacattactt taaaattctg ctttcaaaat
     9481 ccatcaccct ctcattaaca accaactaca tagaaaacac tttcaaaatc cgctctatta
     9541 ttgaactacg cctttatatc caaaaagtta tcaacaaagt ggtaaaaagt ggggataagt
     9601 ggtaaataat ggaaaatttt tatatatttt tacaaccaaa ataaaagtat cccaatggca
     9661 tcatttatag gaaattacac ctgtaaaaca gacgaaaaag gacgagtcac cctgccaacg
     9721 gcttttaaaa aagcccttcc ggcagaggcg catgatactt ttattgtaaa aaaagatatt
     9781 taccaaaaat gtcttgtgct ttatcctcaa aatgagtggg aaaagcaggt agaacaaatc
     9841 cgtgccagcc ttaaccctta caacaaggaa cacatgattt tcttacgcgg gttttataaa
     9901 gacactgccg aggttgagct cgacgctacc aaccggctgc taataccgag gcgattgctc
     9961 gatgaagccg ggataggcaa agaagccgtt ttagcaggca tcgacaacaa aattgaaatt
    10021 tggagcaaag aaagctacga caccatagat gcaggcgaag actttgccat actggccggg
    10081 aaaattcttg gcgggacaaa tcaaaaaacc gaaaagccat gacctaccat ataccagtaa
    10141 tgttgcatga gagcattgaa gggcttcgga taaaaccctc aggctgttat gtcgatgcca
    10201 cttttggagg cggaggccac tcaaaagcca ttcttgccca acttaaaggg gggcgtcttt
    10261 ttggttttga ccgggacgaa gatgccatgc aaaatatccc ttctgaccct caatttacct
    10321 tcgtgaaaca aaacttcagg tacatgaaaa attttctgaa ttaccacgga gtggaaaaaa
    10381 ttgacggcat actagccgac cttggcgttt cgtctcacca gtttgacgag cctcagcgag
    10441 gttttatgtt tagggctgat gcccctttag acatgcgtat ggacaagaac tccaatcttt
    10501 cggccaaaac actgctcaac gaatggacag aagaaaagct ggcagacctc ttttatcttt
    10561 acggcgaaat tcaaaatgcg cgcaaactgg cacattgcat cacaacagca cgtagcaggc
    10621 aagcaataag cactacaggc gagctggttg aatgtatccg cagttgcatc ccgcaaaaaa
    10681 ttgaaaacaa atatatggcc caggtttttc aggctatccg tattgaagta aatggcgaac
    10741 tcgacaacct aaaacaattg ctcgaacagt ctgctgaaat gcttgtccct tctggccgtt
    10801 tggtagttat cacttatcat tctttggaag accgcatggt gaaaaatttt attaaatgtg
    10861 gcaacgtaga aggtaatgaa aacaaagatt tttacgggaa ccgcaatgct ccgttcaaag
    10921 ccatcaacaa atcggtaata accccttcgg atgaagagct gaaaacaaac aacaggtcgc
    10981 gcagcgccaa gctgcgcata gccgaaaagc tttaaaatca ataacatgag tgaagaaaag
    11041 gaaaagaaaa atatagaatt tgtaagcgaa aaagaagaag taaaagagat acaaaagtat
    11101 tccgttaaac attggattac cgggcgagta tttgcttcgg ccgaaatacg cagccaatta
    11161 cctttcattg tttttctggc ttttttgggg tttgtttata tagcaaacag gtatcatgca
    11221 gaaaggttga tgcgcaatac cctcaaacta aaacaagagc tggacgaatt gcgtgctgaa
    11281 tctatcacaa cagcatcaga acttatgttc ctgagccgtc aatcgcaagt acaaaaattt
    11341 gtcaatcagg aaaacctggg gctaaaagaa gccgtagccc caccaaaacg tattaaatgg
    11401 agagactaaa ataaaaatgt caataaaaaa agacatagta tggcgtatag ctctggttta
    11461 tgtagtaatg ctgctggttg gcattgctat tgtcatgcgt attgtctatt tacagttttt
    11521 tgaagtaggg cctttaacca agaaagcaca aaaactatcg gtcagggata tagaaatcat
    11581 cccggagcgt ggtaatatac tggcttgtga tggcaggttg ctggcaactt cagtccctta
    11641 ttacgagata agggttgaca tcagtcgcga aacagtacct tcagggcttt ttattgaaaa
    11701 aatagactca ctggcaactc gtctggcaca aatttttaaa gataaatcat gggggatgta
    11761 caaacgtgaa cttattcatg cccgaaatga aaataatcgt tattatcttc tcaaacgcaa
    11821 agctgatttc gaacagctgc accagttaag aaactgtcct atatttcgcc ttggaaaaaa
    11881 caaagggggc ctgatagtaa tacctaacgg ggtacgtttt atgcctcatg gagaccttgc
    11941 agcacgtact gttggatata taagcaaaag tgaagaaggt acagttgttg ggattgaagg
    12001 ctcatacgat gaagaattgc gcggggtaaa aggcataaaa ctgatgcaac gcgttccggg
    12061 cggaatttgg atgccaatta atagtgaaaa cgagattgaa cctaagaatg gcgttgatgt
    12121 tgttaccacc atcgacctca acatccagga tgtagcccag gaagcattac gcacccaact
    12181 gcaaaaacac aatgcccatc atggcacggc tgtattaatg gaagtaaaaa ccggtgaagt
    12241 taaagctatt gccaacctag aaaaaacaga aaatggccaa tatcgcgaaa tgtttaacta
    12301 tgccatagct gagagtaccg aacctggttc aactttcaag ctggctacca taatggccgc
    12361 tctcgaagat ggatatataa caccagatga taccattgac acacaaaacg gcacctggaa
    12421 aatttatgac aaagaaataa aagactcgca tgaaggagga tacgggaaaa tatcaataaa
    12481 ccaggttttt gaactttcat caaatattgg ggttgccaaa attgttatgg acaattacaa
    12541 aggccgggaa gaacaattta tcaaacgcat ctattcattt gggctcaacg acaaattggg
    12601 cctgcaaata aaaggggaag ggagcccaat gattaaatac cctcatacac aaggctggtc
    12661 aggcatatct atggctatga ttagccatgg atatgaactt aggctgaccc cgttgcaggt
    12721 acttgcgttc tacaatgcag tggcaaacaa tggacgactt gtacgcccgc aatttgtcaa
    12781 agaattgcgg gtaaacggaa ggacaataaa acgttttgaa aaccacacca ttaaatcgtc
    12841 tatttgttct atgtcaacca tccgcaaagt aagaaaaatg atggaaggag ttgttttacg
    12901 cggaacagcc acaaacctga aagactcagt tttacatatt gccgggaaaa caggtactgc
    12961 acaaatagcc cgaaaaaacc aaggatatag acaaaactct cagattgctt atcaagcttc
    13021 atttgtagga tattttccgg cagaagcccc taaatattca tgcattgttg ttgtaaatgc
    13081 cccttcaaat tctgtttatt atggcaattt agtggcaggg cctgttttta aagagatagc
    13141 acaaaaggtt tatgccacct catttgagct acacgacgag ataattggag gacgaaacaa
    13201 cctaaagact gaactccctt atgcctatga cggtaacaga gaacaaattg aagaagtatt
    13261 ggacgaactg ggcatagact atgacgaaaa tgatgagagc caatggattt cattgtataa
    13321 agaagatgat gagtttaaac taaagaaaag aaccgttatc cccaaccttg tacctaatgt
    13381 aaccggcatg ccagctaaag atgctgtata tttagtcgaa aaagccggat tacacccaat
    13441 tgtcaaaggc tatggcaaag tttttagcca gtcaatcggg cccggtagca aggcttcatc
    13501 aggttcgtca ataattctgc aactacgata aaaacataaa ccgtgaaaac attaaaagac
    13561 atactaaaat cggtagaagc caagagcatc aaaggttcga tagacatagc catagaaggc
    13621 atacagtttg actctcgaaa agtagtcaaa aatactgtct ttgtagcggt cccaggcact
    13681 caaaatgatg ggcaccagta tattgaaaag gctattgaat tgggagcaac agctatcatc
    13741 tgccaaactt tgcccaaatc gttaaataca aacatcacat atattgaagt agcagattct
    13801 gcatgggttt taggcatagc catagccaat ttttacgaca atccttcttc taagcttaag
    13861 cttgtagggg taacaggcac taacggcaaa accacaacag ccactatgct ttactggctt
    13921 tataaggcaa tgggatataa agttggctta atttctactg tcatctatta tgtaaatgat
    13981 gaagcttttg aatcgaccca tacaacgcca gacccgttaa tgttacacag ccttatggca
    14041 aaaatggcag atgcgggctg tcagtattgt tttatggaag taagctcgca ctcgatagct
    14101 caaaaacgca ttgcagggct tcactttgat ggtgctattt tcaccaatct tacccacgat
    14161 cacctcgact atcacttaac ttttgacaat tatcttaaag ctaaaaaaga acttttcgac
    14221 tctctgccca aagaagcctt tgccattgtc aatattgacg ataaaaatgg acgtgtgatg
    14281 gtacaaaaca ccaaagcaga ggtaaaaact tatgctttgc agtcggttgc cgactttcag
    14341 gccaaaatta tcgaaagcca ttttgatggt atgcaactga tatttgacgg taaagacttt
    14401 tggacaccat taacaggcaa gttcaatgca tacaatttac tagctatata cagtactgcc
    14461 gttatgcttg gcagtaataa agaagaggtt atgaccaaac ttagcacttt tggccatgta
    14521 agaggacgtt tcgaaacaat acgcgccaaa aacggtatga cagctattat tgactacgct
    14581 cacactccag atgctttgga aaatgtatta aatgcaataa gacaaattcg tcaaagtcat
    14641 cagaaaataa ttactgtagt tggggcaggc ggaaaccgcg ataaaacaaa gcggcctgtt
    14701 atggcaaaag tttgcgttac tttatctgac aaggttataa taacttcaga caacccacgc
    14761 tttgaagagc ctgaagatat tattgcagat atgatgcaag gcattgatgc aaaagacaaa
    14821 ttaaaaactt taaaaataac agaccgccgc gaagcaataa aaacagcggt aatgttagct
    14881 acacctgatg atataatcct gattgcaggc aaagggcacg aaacttatca ggaaattaaa
    14941 ggggtgaagc atcattttga cgacaaagaa gaaattgcac aattactaat gacaaatgac
    15001 aaatgattga aaacaaagaa aatattatag ttagtaatcc ttttcaactg gcattgagct
    15061 ttattgagtt ttgccaaaag ttcgaaaaca cagcaaaatt agcaattgta aagcagctat
    15121 taaaagtagc ggcattgata agagtgaaaa ttcatgagaa actgctcgct caaaataaaa
    15181 acaataaaaa ttattcatta gcaattagta attagtaaac atatgttata ccatcttttc
    15241 acatatatcg ataaagtaaa ccacattccg ggtaccgggt tattcaactt tatttcgttc
    15301 agggcttcta tggccgcact gctttcttta gccattgcat tactgattgg gaaaaaaatc
    15361 atttactttt tacaacgaaa acaaataggc gaagagatac gcgaccttgg ccttgaaggt
    15421 caaatgcaga aaaaaggcac tccaaccatg ggaggtatta tcattattgc tgcaatagtt
    15481 atccctgttt tgcttttagc tgatttaacc aatatatata ccattctgtt gctatttact
    15541 acactatggt tgggtatgat agggtttatt gatgattaca tcaaagttta caagaaaaac
    15601 aaagaagggc ttcatggaaa atttaaaata gcagggcaaa taatattagg acttgttgtg
    15661 ggccttacat tatggctcca tcaggatgtt gtaatccgag ataaaaaagc cagtcataaa
    15721 cagaaaatgg agaatgtaca aacaacagac caaacaatag caaagaaagt tgacattaag
    15781 tcaaccgcca ctactattcc gtttgtaaaa aaccacgaat tcaactataa caaattactg
    15841 tggtttatgg gcgaaaatgc caggaaattc cagtggataa tatacattct tgtcattatt
    15901 gtgattatta ccgctgtttc aaacggtgcc aatataacag atggcctaga tggtttagct
    15961 acaggcactt cggcaatcat aggctctgtt cttggtattt tggcctacgt ttcaggtaac
    16021 atcatttatg cagactacct caacatcatg tacatcccac atattggaga gttgatgata
    16081 tttgccgcag cttttatggg agccacaatt ggttttttgt attacaattc gtaccccgca
    16141 caggtattta tgggcgatac tggcagtttg actttaggag gttttattgc tgtatttgct
    16201 attgtaatac gcaaagagct tctcattcca atactttgcg gcattttcct tgcagaaaat
    16261 ttatcagtaa tggtacaggt aagctacttt aaatatacca aacgtaaata tggcgaagga
    16321 aaaagaatat tactgatggc cccgctccac caccattttc aaaaaaagaa ttatcatgaa
    16381 gccaagattg tcacacgctt ctggattata ggaattctgt tggctattat aacattggct
    16441 acaataaaaa tcaggtaaat aatacaacat gtcaaatatg aaaaggatag taattttagg
    16501 tgcagcagaa agtggtgttg gtgctgctgt ccttgcaaaa gccaaaggct ttgatgtatt
    16561 tgtttctgat gccggcaaaa tcaaagaaag ctacaaaaac atgctcatcg ggcatcaaat
    16621 agattttgaa gaaggcaaac attctgaaga ccttatccta aatgctcacg agattatcaa
    16681 aagcccgggg atacctgaaa aagctgaaat ggtaaaaaag attcgcacaa aacaaatacc
    16741 tgtgataagc gaacttgaat ttgccgcacg ctacaccaat gcttataaga tttgcattac
    16801 aggtagcaat gggaaaacca ctaccacctc gcttatcttc cacatgctaa aaaaagccgg
    16861 attaaacgta ggattagcag gcaacattgg ccaaagcttt gcctaccagg tggctacaca
    16921 agacttcgat tattacgtat tggaaataag caactttcaa ctcgatgaca tgtacgaatt
    16981 caaggctgac attgccattt tgctcaatat taccccggat caccttgata ggtacgacta
    17041 cgacctgcaa cgctacatcg actcaaagtt tcgtatcacc caaaacctta cagacgaaga
    17101 atatttcatc tactgctcag acgacgagct tactgcccgt gagatggaaa aaattgtcac
    17161 caaagcaaaa caactacctt tttcattgaa agaaaaaaaa ttgcccggcg catacgttga
    17221 taacaacttt atacatattg gacttgacga tgttgacttt tccatgtcta ttgacgagct
    17281 gtcacttaaa ggaaaacaca acacatataa ctctatggct gccggaattg caggcaacgc
    17341 tttgcgtatt cacaatgaaa tcatccgcga agccttatca gactttaaag gcgtagagca
    17401 caggttagaa atggtctgca aagttaatga tatctctttt atcaatgaca gcaaagcaac
    17461 taacgtaaac tctgtctggt atgcccttga atgcatgact acccccgtta tttggattgc
    17521 aggcggtaca gacaaaggca atgattattc cgaacttttt gaacttgtaa aatcaaaggt
    17581 aaaggcgctt gtttgtcttg gcgtggacaa ctcaaagctg cacaaagcat ttgatgataa
    17641 aattaaaatt atacaagatg catcctcgat gcaggaagct gtccggaaag cattccactt
    17701 ggcctcagca ggagaaactg ttttgctctc ccctgcctgt gccagctttg acttgtttga
    17761 aaactacgaa gaccgcggaa gacagtttaa aaaatatgta aaagaattgt aagatatacc
    17821 atattattat gtttgaacga gtagaaaaaa acctcaaagg tgataaaata atctgggcaa
    17881 taatattgtt gctctcgtta ttgtcattac tggcggtata cagctccacc ggaacgcttg
    17941 cataccgcta tcacggggga aacactattt actacttctt caaacatgca tcatacatgt
    18001 tattaggatt aggcgctata tatataacac acctaatccc ttataaatat tatttacgcc
    18061 tggcccaaat attcctttac ctctcagctc ctttactatt gctaacctta gtcatgggaa
    18121 catcgcttaa ctctgcatcg cgatggttaa cagtacctgt catcgggctt accatccaaa
    18181 catcagattt tgccaaaata gcgctaatca tgtatgtagc caggactctt gccttgaaac
    18241 aagatgaaat aaaggatttc aaaaaggcat tcctcccggt aataatccct gtagggatta
    18301 tatgtaccct tatccttccg gcaaacctat ctactgcatt gatgatattt atggtgagtg
    18361 taatactaat gtttattggc cgggtatcaa taaagcacct ttctttattg ggattgtgtg
    18421 gcattatatt tttatcatta tttatcataa ttgccccaat gacccataca aaccttggca
    18481 accgcgttaa aacctggaaa caccgtattg tcaactttag tgggaataat gacgataaaa
    18541 aggaaaccaa cgatgcaaac ttccaatctg agcaggccaa aatagccgtt gcaaccggag
    18601 gcatattagg caaaggcccc gggaacagta ctcaacgcaa ctttttgccc catccttatt
    18661 ctgattttat ttatgctata atagttgaag aatatgggct tgtaggaggt atttttgttt
    18721 tatcattata tttagcttta cttttcaggg cgggagttat tgtacgaaaa agtgaaagaa
    18781 cttttccggc cttccttgct ataggattag cattattact cgtatttcaa gctatgataa
    18841 acatggcggt atcagtaaat cttgtccctg taacaggaca aacgctccct ttagtaagca
    18901 tgggaggttc gtctatgtta tttaccagcg cagcaattgg cataatcctt agtgtttcgc
    18961 gctctataat aaaagaaaaa gaagttaagg aagaaaacaa agaagatgaa aataaagaaa
    19021 aaaaagaaga cgaaaacaat attaataccc aagcaaaaga gaaagttcag gaaaaagaaa
    19081 cagaataaat aaaaaacaaa caatataagg gattaacaag ctaatatgca aaaaaataga
    19141 gaaataagag tcattatatc tggcggaggg accggaggac atatatttcc ggccattgct
    19201 attgccaatg caataaaagc cgaaaaaaca aatgccgaaa ttctatttgt aggagctctg
    19261 gggcgtatgg agatgcagaa agttccggcc gccggataca acattgtcgg gcttcctatt
    19321 atgggcttca accgaagctc cttatggaaa aattttgaag tattagccaa gcttgtaaaa
    19381 agccttttta aagcccgtaa gattatcaaa gatttcaaac ccgatatggc cattggagtt
    19441 ggaggttatg ccagtggccc actcctttgg atggcctctt tttcgaaggt gccaattttt
    19501 attcaggaac aaaattcata cgcaggagta actaataagt tgcttgcaaa acgtgcaaaa
    19561 aaaatatttg ttgcctatcc cgacatgcag aaatttttcc ctgccaaccg tattattata
    19621 agcggcaacc ctgtaagagg aggcctaaac aatgtttcaa acctgaaaca cgaagctatt
    19681 gactttttca aattagaccc ggcaaagaaa actatacttt gtattggagg aagccttggt
    19741 gcaagaactt taaacgaaag cattattaat aatatcaaca aaatagttga aagcaattat
    19801 caacttattt ggcaatgcgg agcatactac catgcagctt gtcaaaaaag catagaaaac
    19861 ctaaaaaatc aaaatatcaa gctttacgac tttatcaacc gtatggacct ggcttatgct
    19921 gctgccgatg tagtcattag cagagccggg gcattgagca tttcagagct ttgtcttgta
    19981 agcaaaccgg ttattctggt tccctcacct aatgtagccg aagaccacca gacaaaaaac
    20041 gcattagccc ttactgaaaa aaatgcagca gtaatgatta cagataatga tgcccccaaa
    20101 aaacttattg acgaaacctt tgcattactt aacaacgaaa acaaatctca aatgctggcc
    20161 aacaatattt caaagtttgc catgcatgat gcagcaaaga atatagcacg cgagattatt
    20221 aaaaatgtca aaccagaaat gactaaccaa tgacattcaa aattgaaaat ataaaaaatg
    20281 tatatcttgt aggcattgga ggcattggca tgagtgcatt ggccaggtac tttaaaacca
    20341 ttggccgcaa tgttgccggg tacgacaaaa cccctacccc actaacttct aagctaacag
    20401 aagaaggtat tgatatacat ttcgatgata atttggcgct tgttcctgaa tacatcaaaa
    20461 aagaaaaaga caatacactt gtcatattta cacctgcagt cccttcatgg catgtagaac
    20521 ttaatttttt caaaaacaat ggttttacaa ttatgaaaag ggctgaagta ttagggcata
    20581 tattttcaca aggcattggg gctgctgtag ccgggactca cggcaaaact acgacctcta
    20641 ccatgcttgc ccatatttta cacaactcgc aaaagcaatg caacgcattt ttaggcggta
    20701 tagcccgaaa ttacaattcc aacctgctcc tttcagacaa aagtcaaata gtagtagccg
    20761 aagcagatga gtacgacagg tctttccttc gcctgtaccc aactgtagct attgtaacct
    20821 cggttgaccc tgaccacctt gacatttatg gttcgtactc tgaagtaatc aaatctttca
    20881 acgactttgt aaaccagata aaaccaggag gagctttggt ttataaaaaa aatattgcca
    20941 ttgatttaaa caaacttgaa aaaatcaata aatacagcta cgatataaac aatgacgctg
    21001 acttttcagc cagaaacctg agaattgaaa gccgaggatt ctgttttgac ctttataccc
    21061 cagagagaaa ccttgaaggc atccagttac gcattggtgg cgctatgaac gtgctcaatg
    21121 caaccgcagc agctgctgct gcgcatttat taggtgtcgg ttacgatgaa atcattgcag
    21181 gacttgaaag ttttgaaggc gttaaacgcc gttttgaaat tcatttgaat gaaaatggca
    21241 ggacctacgt ggacgactat gcccaccacc ccgaagaatt aaaggcttgc atcaaatcgg
    21301 cacgccagat attcccaaac agaaagcttt gcggcgtatt tcagccacat ttgtacagtc
    21361 gcacccgcga ttttgcagat gaatttgcca aaagtttaga tatgcttgac gaaataatac
    21421 tactcaacat atatccggca cgcgaagagc caattccagg cattacctcg catgtcattt
    21481 ttgaaaaaat gaaaaatgcc aacaaagtcc tttgttctaa agaccaatta ataaaagaat
    21541 taaaaaacag ggacattgac gtattgctta ctttgggggc aggtgatgtt gaccaaatgg
    21601 cagcagatat ttgcaatatt atcaaacaac aggcatgaaa aaggcaataa acatatcgct
    21661 ttgggttgta ctgatagctt atttgggggc tatttacggc tttgttgaag aaaaaaacaa
    21721 aggaatcatt tgtaatgata tcaatgtatc tgttgtcaac aatgaaacta ataaactaat
    21781 cagcaacaaa tccattttgg caaccataaa aaacagccat atccctatta tcggcaaaca
    21841 agtaaacaaa atcaacacct ttgcccttga aaaccttttc aaatctaatg cgacaataaa
    21901 acaagtggct atttatacca ccatagatgg tatattaaac gttgaagcag agcagcgcga
    21961 acccatactt agggtaatga atgcccgtat gcaacagtat tacattgata acgaagcata
    22021 tattatccca atacgctttg aagccccggc ctattgttta tttgccaacg gcaatataaa
    22081 agaaacgttt aacgtaaacg aagcgcatac cgaaaagagc ttgttgatgg acacgttgaa
    22141 caacaacaaa aacaatatta gcagcatatt taagctctct caatatatta attcccataa
    22201 cttctggaaa aaccatatat ctcaaattta tgttgacggc gataacaatc tcatggtcat
    22261 cccccgagtt ggtgcacatg taattatcct cggagatata accaatcttg atattaaatt
    22321 caaaaaattg cgctctcttt attatgcttt taatgaaatt ggatggaacg actataaaac
    22381 cattaaccta aaatacagta accaaatagt ttgcacaaaa cgttaatatt atgatacata
    22441 aagaacaaat tatcggagca gtggacatcg gaaccacaaa gattgtaact attattggaa
    22501 aaatcaacgc taatcagcgc cttgaagtcc ttggcctgtc atgcacagaa tcaagagggg
    22561 tcaaaagagg tgttgttcaa aacattgaag aaacagtaat gtctatcaaa cataccataa
    22621 aagaagctga agcccaggca ggatataagc tcaacgaagt gtttgtaggt attgccggtc
    22681 agcacattca cagcattaaa agcagtggtt atttgttacg cgattcttat gacaacgaaa
    22741 tcaccgctga agaccttaaa cgtctcgaaa acgatatgcg caaaatacct gttgatgtag
    22801 gagaggaaat catacacgtg ataccacaaa gctatactgt ggacaacgaa cccgatatca
    22861 aaaaccctgt gggcatgttt gggaaacgca tagatgcaaa ctttcacatt gttattgctt
    22921 ctgtaacttt tgcaaataat ataaaacgct gcatcgaaaa atcaggatta caagtaaaat
    22981 cattgatcct tgagccttta gcctcatcag aagccgtttt gacagaagat gaaaaagaag
    23041 ttggagtagc aatgattgat attggaggag gcacatctga ccttgcagtt tactatgaaa
    23101 atattataag acatactgca gtaatacctc ttgggggtaa cattatctct aaagacttaa
    23161 aggatggact ttgcattctc ccgcgccatg ccgaaatgct taaaattaaa catggatctg
    23221 cactgcaagc attagcccaa cccaacgact ttgtttcggt accgggcatc tcaggacgag
    23281 aacctcgcga aatatcaact gcaacaatag cgggcattat ccagtgccgt gtagaagaaa
    23341 ttttaaactt cattgccctc gaaattgagc gaagcgggtt tcaagacaaa cttagcgctg
    23401 gtattgttgt tacaggcggc ggttctatgc tgaaaaatat ccgtcagctt atcaattttc
    23461 gcacaggcat ggattctcga ataggttttc ctacagaaca ccttgttgct tcatccgtag
    23521 cagatgtcaa ccatccaaaa tacgcaacag caataggcct tgtttttaaa ggatatcaat
    23581 atcttaacga acacaaagaa gtgatgagct ttactaatat tggagaagaa attaccgcag
    23641 gttttcaacc taacacagct ccaacttcag aaaataaaac agctgttaag gaagaagaat
    23701 ttaaagaacc cgaaaataaa gacattacca aatcaaattt tctcaattct ttattataca
    23761 aatttaccgt tatgtttgat gaaaacgaca caaaaatgta gccattaaaa aaattataaa
    23821 ccctaatcat tatggaagat ttaatcaact ttgacataca agccaacaaa tcatctatca
    23881 tcaaggtgat aggtgttgga ggaggcggta gcaatgctgt taatcacatg tttcgcatgg
    23941 ggattaaaga tgttgacttt attgtatgta atacagatgc ccaagctctt gcaaacagcc
    24001 ccgttccaat aaaaattcag ctgggcaatt cgctcaccga aggacgtggt gctggcaaca
    24061 aaccagatgt aggccgtcaa tctgctattg agagccttcc tgaaattatg gaagcccttt
    24121 cgatgaatac aaaaatggcc tttatcactg ccggaatggg cggaggtaca ggtaccggtg
    24181 cagctccggt tattgccaaa gctgttaaag aacaaggcat actgactgtt ggtatcgtta
    24241 ccattccctt caggtttgaa ggcccggtgc gtatcaacca ggcaattgag ggtattaatg
    24301 aacttcgaaa ttacgttgac tcgctgttgg ttattcacaa cgaaaaactc agagaattat
    24361 atggcaatct caagatttca gaagcatttt ccaaagccga caacatttta actgttgctg
    24421 ctaaaggcat tgccgaaatt atcactgttc atggttatgt caatgttgac tttgctgatg
    24481 tacaaacgat tatggccaac agtggtgttg ccatcatggg ttcagctacc gcatctggac
    24541 aagacagggc ccttgaagct attcaacaag ccctcacctc tcctttactc aataataatg
    24601 atattcgcgg ggcaaaaagc atcttactca acattacttc tggcgaagat gaaatatcaa
    24661 tggatgaagt tggtattatt actgactttg tagtcaactg cgtggacaaa gatgcgctta
    24721 tgatttgggg tacaggtatt gacccttcac tcgacgataa aatttctgta accattattg
    24781 ccacaggttt ccctgacaac tccatcccag agttgatcac ccgtaaagct caaccagcaa
    24841 aagttgccgc aacaataaat gagcccttta catctaagct tccatttgaa gtaaagcata
    24901 gtaaagaaaa ctttcaacct atccaaaaaa ccattgagtt tgattttggt tcatcggcat
    24961 tagatgtacc cctcgttgaa agtcctatca accaaagtaa agccaatgaa aggctttcgg
    25021 aaatcaaaaa acagtcggta aaatctgatg gaatactacc taatcatctc gaccctaatg
    25081 taatagaaga attggaaaat actcccgctt ttatgcgaaa aaacattaag cttaaagaat
    25141 ctaaatcagc atcagggcca gaaatatcaa ggcttacact ttttgatagc gatgaagata
    25201 tcattaaaat tagtgccgaa aatccttatt taaacaaaaa cgtagattaa caaaacgttc
    25261 atagctccta ccaatgagaa ctaaggctct gtcttattct cattggtatt ttttttaaat
    25321 aaa
//
LOCUS       DAOSPF010000052        25217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_1974_length_25217_cov_9.027581, whole genome shotgun sequence.
ACCESSION   DAOSPF010000052 DAOSPF010000000
VERSION     DAOSPF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25217
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_25217_cov_9.027581"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1390
                     /locus_tag="PK252_10440"
     CDS             617..1390
                     /locus_tag="PK252_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT15167.1"
                     /translation="MITNNKQWYEQLFENFSNQYENEDFTKGTIGEVDFLEQEFGFNK
                     NAKILDVGCGTGRHSIELAKRGYQPEGFDLSENQLAKARENAAKAAVVINFFQADART
                     FDFKEKYDYAIMMCEGSFPLMETDEMNFEILSNLYKSVKPGGKIIFTTLNGLFPLFHS
                     VKDFINDNSLCQSFGNTFDLMAFRDYSNYTFKDDAGVEHTIKCNERYYVPSEITWLLK
                     SLGFKKVNIFGAHLGNFSRSHALTTEDYEMLVIAEKPAK"
     gene            complement(1451..2698)
                     /locus_tag="PK252_10445"
     CDS             complement(1451..2698)
                     /locus_tag="PK252_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT15168.1"
                     /translation="MNKYFKILQLSENASEEDIRKAYRKLAKEYHPDVNKSKDAHEKF
                     IEISEAYEILLSQRNKRLAKEQPYETAASKAAEEAEYERFREEIRERASRQAQMRYEE
                     FVRQNEAFQKSGLKDLGLLFTIFIRLLLLPLTAALIVVPFVVAFHTVWYAVFILLLTG
                     PFAAILLWYMKDKRKGFFMPGNFYYNFKRIKRIFYETTACEKECFYCKGHIGNHKPFV
                     LTLIKLKDIRLSTKGFRQHNANYVTENVQVLVPRSQKALIIHTLNIGIKVLSILLCLF
                     LLPVDSLVWRFIFGIISGGTIAALVNLFTRTKSSVSYLYSFGFVIRVLVWLLPLIIIS
                     GFSFSPLNITTSDFIYLTVVCIVLFDCLLMQLLNAILGKQAYLPLVRQYEDVKNRFDE
                     GYKVYNDIPIISVVFPLYKWIFG"
     gene            complement(2943..3440)
                     /locus_tag="PK252_10450"
     CDS             complement(2943..3440)
                     /locus_tag="PK252_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT15169.1"
                     /translation="MLAFSVNAFSQFQFKGIGAGLALGSKAAISDEGDDKMGFGININ
                     ALAQIVEKIDAEVSLSYYFPSEPVDGIKFQLTTISLNGHYNFIAEKNMTVYGLAGLNY
                     SIGKVEMELFGTSISDDESEIGLNLGAGGAFGITDNIDLTGQVGYTFGDADQLFLNVG
                     VMYKF"
     gene            4324..6663
                     /locus_tag="PK252_10455"
     CDS             4324..6663
                     /locus_tag="PK252_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOT15170.1"
                     /translation="MKKVLIIMLVMASCMQLQSQDRFIRRNGQDLFLNGMNLAWMSYA
                     NDLGAFNEAAFIKAVTSIRESGGNTIRWWLHVNGQNSPQFTGDKVSGISQTNLANLKR
                     ALDIAAENGVVLDLCLWSFGMLDKTYTAAVLDRNTKLLTDTAYTNAYIRNALLPMVRY
                     VKNHPGLLCWEVFNEPEGMSTEYGWSDYQHVNMSYIQKFVNLVAGAIHREDSTLKVSN
                     GTVTLRTATDMDGFTNFYRPDRLIKAGGDSLGTLDFYMVHYYTANGTQYSPFRKPASY
                     WKLDKPLVIGEFSAKGITEAGAPAQTPEELYNWAYDQGYAGALSWTYTNHDGNGGLPD
                     CGAAMLKIAFNHSDHVILKDAKPFNFNPYQKKPLYIGWKYKGTKDTIRISPLLAYITD
                     KEDSSLLTFTVKSTTNPVAANIKSNDTLELISSEATEAGVYTTLITATDTGNKSITLP
                     VFMSVIDTTSDDVLLNRPLYVSSIESTQYYGHFANDNSDTTRFSTEYADNQSMLFVLD
                     KEYTLQRMLMKWEAAYGKAYSIELSTDSANWTKVYSCTNENGGYDKIVFSPTKAKYVK
                     VNLEKRGTQWGFSLFTIQAFEDAIANNAPYFLPKISDTTFYTRTRFTLNLSQSAKDSD
                     LGDRIKYRLALLNNANLPTFITFNDSTYVLTGRPTSTDTTVLTFVITATDIEGAEATD
                     TFSVKITQKTAIENIARNNVRLYPNPVSDKLIIENAENIDCILIFSTDGKLLNKYAVK
                     TSVLNGKYELYLGQLNAGNYLVHLQSKSGNTIAPITVIK"
     gene            6670..7011
                     /locus_tag="PK252_10460"
     CDS             6670..7011
                     /locus_tag="PK252_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOT15171.1"
                     /translation="MHELSIAQSVIDTIKKNVPSDLLPKADKVKLQIGTLSGIELDAL
                     TYSFGILVQNTAFPQLAMEIDLKEGKGQCQACGTEFTYNKYGIPCPQCGGFSIKIIDG
                     KQMKIINITMK"
     gene            7066..8037
                     /locus_tag="PK252_10465"
     CDS             7066..8037
                     /locus_tag="PK252_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT15172.1"
                     /translation="MTNLSSIRLPVEKEIAAFEKHFSDSLDTQIPLLDTITNYLLRQK
                     GKQIRPLFVFLTAKTLAEPTESTFHAATFIELLHTATLIHDDVVDESYQRRNHFSINA
                     IWKSKVAVLVGDYFLSKGLLLSVKHKEFELLEAISQAVREMSEGELMQIQTSKKSILK
                     VENYFEIIRKKTASLIASCCACGAISVKADEQTIEKMRQFGFYIGSAFQIKDDLLDYQ
                     EKDLIGKPKGNDIKEKKYTLPFILAVQNNQKEKSRILRLFNQSHKPTETIEEISKFVI
                     DNNGIADSIKYMNEFRSKALDILSEFPDNEAKKSLILLVDYIIERKH"
     gene            8405..8644
                     /locus_tag="PK252_10470"
     CDS             8405..8644
                     /locus_tag="PK252_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15173.1"
                     /translation="MTRQCISFTKPNDEWLKLQVDSKEYSSKSEVVNDLIRQARKQQV
                     QIDLIRAKLDKSEASGFTDLSKEQILKQSKASLNG"
     gene            8637..8921
                     /locus_tag="PK252_10475"
     CDS             8637..8921
                     /locus_tag="PK252_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT15174.1"
                     /translation="MVKYKLSNEAKEDLIRIHHYGVEKFEITQADEYFDAFFEYFDII
                     AQRPLSFESVDYIKKGYRRCVCGVDSIYFRIEYGTVEIVQRSLSLKAVHY"
     gene            complement(9014..9346)
                     /locus_tag="PK252_10480"
     CDS             complement(9014..9346)
                     /locus_tag="PK252_10480"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT15175.1"
                     /translation="MDTPKRLVIISPEAANDIQEIYLYGYETFGKNLAESFKSELLSM
                     IRCLSTSYLSYPECRQIPTKSKMYRNIIIGSYLIIYRITPQRIEVLKAVSSRKSVTKI
                     RSSRRIKL"
     gene            complement(9322..9492)
                     /locus_tag="PK252_10485"
     CDS             complement(9322..9492)
                     /locus_tag="PK252_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15176.1"
                     /translation="MKSVKTKESKRINWAVPGKVTHEEFMAAIKKAEEGPFMTIEEFE
                     NKFEEWIRQKGL"
     gene            complement(9548..11359)
                     /gene="uvrC"
                     /locus_tag="PK252_10490"
     CDS             complement(9548..11359)
                     /gene="uvrC"
                     /locus_tag="PK252_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT15177.1"
                     /translation="MELSTEIQKTLENLPHLPGVYQYFNADHKIIYVGKAKDLKKRVS
                     SYFAKEHHDNAKLRILVRNIHEIRFIVVASESDALLLENNLIKEYQPRYNILLKDDKS
                     FPWIAIKRETFPRIVYTRQLIKDGSEYFGPFTSTVMVKTLLSLIKQLFPLRTCKLDLS
                     LDKITRKMYKPCLEFHMGNCLGPCIAKQSLDDYMENIQTIRDILKGNLNQVSQHLKQM
                     MNAFAKDLDFENANLIKEKIAILDNYRSKSTIVNPAIHNVDVFSLASSDKMVVVNYLR
                     VLNGAIVQVHSMFTSLKLDEDLSEILSMAILEMREKLSSNAREIVVSQMPDVQMQGIT
                     YTIPKTGDKLKLLELSMRNSKMFLQEKLKQSSEMAEKKGAKANIIVQQMKDVLCLEKL
                     PLHMECFDNSNLQGTNPVASCVVFKNGKPSVKDYRHFNIKTVTGIDDFASMKEIVYRR
                     YKRLLDENAPLPDLIVIDGGKGQLGAALESLDKLNLVGKIEIISIAKRLEEIFKPGDS
                     IPLYVDKKSPVLKVIQHIRNEAHRFGITFHRNKRSNTWMNSEFENIKGIGEKTVNELF
                     IKFKSAEAIHQASLEELAEVIGMAKAKILREHLGKDN"
     gene            complement(11362..12975)
                     /gene="ade"
                     /locus_tag="PK252_10495"
     CDS             complement(11362..12975)
                     /gene="ade"
                     /locus_tag="PK252_10495"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOT15178.1"
                     /translation="MKISGNIVDVFSKRIFSGTLMIEKGHIKDIVEEDNIYSNYILPG
                     FIDSHVHIESSLLTPNEFAKAALKNGVVGAVCDPHEIANVAGVNGIDFMLQCTKDVPF
                     HFLFGAPSCVPASPFDSSGAVLSSKEIEHLFSTKRAFFLAEMMNFVGVVNEDREVLAK
                     LSLSKKYGFPIDGHAPGLSGQELIKYANAGISTDHECTNVHEALEKIKYGMKILIRNG
                     SAAQNLADLAPLFTNCADDLMLCSDDIHADHLLEGGIRSSVKWLVGHGYDIFDILKVA
                     CINPVQHYKMDCGLLRVGDKADFIVVDNLNDFNVQEVYFRGIPSMHFQQKSLPGSFVF
                     PFRDKKIAVDDLLMAKKSMSMRVIEVIDKQIITNELLYQWPDDALNNFESTVSEDILK
                     LVVCNRYENKPPVIAFVKGFKLKYGAIASSVAHDSHNIIAVGVRDEDIVGAVNTIIEN
                     KGGLAFFSEQEKEFLPLKYGGLMTGMPAEEVAEAFNRLEKKIAEAGCSLHSPIMNLSF
                     LALSVIPTLKLTDKGLFKIAEYSLVDLFC"
     gene            complement(13433..15289)
                     /gene="mnmG"
                     /locus_tag="PK252_10500"
     CDS             complement(13433..15289)
                     /gene="mnmG"
                     /locus_tag="PK252_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT15179.1"
                     /translation="MIESYDVIVVGAGHAGCEAAAAAANMGSSTLLISMDMTKFGQMS
                     CNPAIGGIAKGQIVREIDALGGLTGIITDKSTIQFRMLNRSKGPAMWSPRAQCDRVAF
                     SVEWRKALEAIPNLFFWQDSVNGLIIENGVAIGVTTTIGVKFFAKAIILTIGTFENGL
                     IHVGRNHVPGGRVSEPASMGISECLKLAGFEVGRMKTGTPARIDGRTVDFSKLQIQES
                     EEGKFSYLHEVKPALKKLPCYIVNTNEEVHNILRTGFHDSPLFGGIIKGRGPRYCPSI
                     EDKIVTFAEKNAHQLFLEPEGEDTHEYYLNGFSSSLPWDIQYMALRKMVGLENVKIFR
                     PGYAIEYDYYPPTQLKNTLETKYIENLYFAGQINGTTGYEEAAGQGLMAGINAHLKIN
                     GKEPFILSRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEFRILLRQDNADDRLTKKGFK
                     IGLASEKRYKDWLNKSEQKEIVLKYIASNSVLPDEINPFLLQYGCTTILQSVKIREIA
                     MRPQFTLLSLFQHLHHLGKYNFSLDEQFLFEIEIALRYQGYIDREQNIANKIKKLEGI
                     KIPANFDFERLNITIEARQKFKKYAPQTIGQASRIPGISPSDINVILIHIGR"
     gene            complement(15282..15626)
                     /gene="ybeY"
                     /locus_tag="PK252_10505"
     CDS             complement(15282..15626)
                     /gene="ybeY"
                     /locus_tag="PK252_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT15180.1"
                     /translation="MIERIEHCKIENINFIFMPDRNIRLLNIKYLNHHYPTDVITFDY
                     SQMNEIGGDIFIGLHTVKYYAHKNAIPYQKEIYRVMVHGVLHLAGYKDKSIRERNLMR
                     RKEDFYLRKLYD"
     gene            complement(15702..16100)
                     /locus_tag="PK252_10510"
     CDS             complement(15702..16100)
                     /locus_tag="PK252_10510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15181.1"
                     /translation="MEKNIDISSKIENLRQVEKFVDEISSECKLNSELYGNLLIATLE
                     GANNAIIHGNKLNEQLMVNINAKCENNILEISIKDQGKGFDYKNIPDPTAPENIENVS
                     GRGVFLMTRLSDNIEFFNNGALVVLKFKIN"
     gene            complement(16264..17445)
                     /locus_tag="PK252_10515"
     CDS             complement(16264..17445)
                     /locus_tag="PK252_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT15182.1"
                     /translation="MKNFLIILFSLFSLFVSGQRQKDLEKERLKKIEEIDRIEKLISK
                     TNEATQSNMNAIELIDRKIVLRNEIIENLNYQVESLDSQIVYLNGNIDFRKNEISSLK
                     AEYAKTIYQIYFHFKDYNFLMFLLSSQSFNQAYRRLYYFRQFTEHRRILLKEIVTEVD
                     TLKRSVSYLLSVRKDKANVLALREDEKRKLEKDKSLLSATIVENKKKINKFKKELETL
                     NLVKKKIEAEIYRIIEEEARKKANMDKKQTEEVVLLSNEFSKNKNKLPWPVANGTVYS
                     YFGEHKHPYLSGVKINNFGIDISFDCNQPVKCIFKGVVSKIFAIKGANTAIIIRHGDY
                     LSVYQNLSSVVVKVGDNVDTNQKIGFCSCVEAGNGAANLHFEIWKEFTKENPIYWLKN
                     Q"
     gene            complement(17449..17868)
                     /locus_tag="PK252_10520"
     CDS             complement(17449..17868)
                     /locus_tag="PK252_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15183.1"
                     /translation="MNFNKELYSVAFRKSDSVLYLKVYIDGAVKVADIIAYKDSVHIK
                     SSFVDISPVYQKLFDDCFLYYLLDDLLINGLSVSANVPYKVDKIVNPDNFKYSVSLLC
                     EVNSKFTQLLVNKYVLLNGRIKVKQFTISDEIKVSID"
     gene            complement(17973..19655)
                     /locus_tag="PK252_10525"
     CDS             complement(17973..19655)
                     /locus_tag="PK252_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15184.1"
                     /translation="MKKITFLLISIFTFGICATAFSQANKKDVQFLFIEAIANKKLGN
                     YYNAAVTLDKLIRADSTCLACYYEMADIMMKSKDQRNAIFYASKAFEKDSSNYWYALL
                     FADVLKENGYFIHAAAKYKYLLDKFGEDPDIMFDYGLMLKFSGNTKEANNVFFELYQK
                     YGLTRELGLMLADIFNEMDKPKEGIAILNKLVKIDSFDAQIAVKYGDLYFSMKSKSLA
                     EKYYAEALMLDSLNSYILYRYIYFLFQTKNSQAFQQKLYSTITNDSLSIEFRKDVFSK
                     LSFISQREPKLYFFIEKVLQSVNVNLYVGFADVFADFYELNRNFKSALMCTKALYNGN
                     KNELRTWERLLYYFNNSSQFDSTLWYYKNAEATVGVSAFMVYMAAIAAYSKENYKLAV
                     NIYLNGLQNTKFDNDQLQRNFLLGLGDSYYMSGNLDSAIVVYAEVFKKYKPDNLLYNN
                     LAYYLAEKGVDLIKAAEMSRLTITAEPKNPTFLDTYAWICFKLGNYTDALFYIKETIK
                     YSKYFSKDVYLHLYAISVCTNEKGLAAKALKALKKNLGQKFQDKLVSDIKCK"
     gene            complement(19662..20093)
                     /gene="dut"
                     /locus_tag="PK252_10530"
     CDS             complement(19662..20093)
                     /gene="dut"
                     /locus_tag="PK252_10530"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT15185.1"
                     /translation="MKVKVINKSKHALPQYQTAHSAGLDLCANIDTPLKLKPLERVLV
                     PTGLFIELPVGYEAQIRPRSGLAFKHGVTVLNTPGTIDADYRGEIKVILVNLSNTEFE
                     IFDGDRICQMVIAKHETVQWVDVEEIADSERGHGGFGHTGK"
     gene            complement(20095..21570)
                     /locus_tag="PK252_10535"
     CDS             complement(20095..21570)
                     /locus_tag="PK252_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT15186.1"
                     /translation="MSQIKKFAGQAFVYGLSSIIPRFLSYLLVPIYARIFLPNEYGVI
                     ADLYAIVAILNIIVTYGMETAFFKFSNQSEFRNVYSTAFFSLLFSSSLFLFLGLIFNK
                     NIAAFIDYSGNPEYIVYFILIIFFDAICAIPFVKLRNDNKALKFATLKILNVVVNVAL
                     NVVLLIILPWMSGKFHVFHFELNVKYVFIANLISSLFVFLTLIKDFPIKFKFDFRLWK
                     KMMVYAFPLLIAGLAGMINDNIDRQFIKYLSPVGHDAMSDLGIYFANLKIAVILVVFT
                     QTFRFAAEPFFFKRQEDKSSKDTLAQVMKFFILVCLVVVLFTMANISIFKYYIGEKYW
                     SGLSIVPVVLGANVLVGVYVNLSIWYKLSGKTMYGVYFIVFGCLLTIILNYFLVPVYG
                     YQAAAYNRFISYLLMVLLCYWFGTKNYSIPYDVKRIGKYLIIFLVGLFIILFLNSLHL
                     SFLIVVNNLLLVIFVYWILKSENLWLVTWNYVKNLKKLIIK"
     gene            21714..22721
                     /gene="pheS"
                     /locus_tag="PK252_10540"
     CDS             21714..22721
                     /gene="pheS"
                     /locus_tag="PK252_10540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT15187.1"
                     /translation="MKEAIYQHLKDVDSYTINNSAELEEFRLKYLSKKGILQQLFESF
                     RTIPNEEKKEIGKLINELKQKVEGKFSEIQSKLEADSTENTAIDYTLTPVSMKLGTRH
                     PLSQVRNEITSIFKTLGFTISEGPEIEDDWHVFSALNFAEEHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQIRAMETQKPPLRIIMPGRVFRNEAISYRAHCIFHQIEGLYIDKNVSF
                     ADLKQTLLYFAKELFGADTKIRLRPSYFPFTEPSAEMDVSCSICKGKGCNVCKHSGWL
                     EILGCGMVDPNVLANCGIDPNVYTGYAFGMGIERITMLKYAVKDLRMYFENDLRFLNQ
                     F"
     gene            complement(22761..23792)
                     /gene="ruvB"
                     /locus_tag="PK252_10545"
     CDS             complement(22761..23792)
                     /gene="ruvB"
                     /locus_tag="PK252_10545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT15188.1"
                     /translation="MDNNFEYNKEKFVTDKDFDKKIRPDHFSKFQGQDKIIENLKVFV
                     QAALLRKETLDHVLFHGPPGLGKTTLANIIAHELNVNLKITSGPVLDKPGDLAGILTS
                     LETGDVLFIDEIHRLSPVVEEYLYSAMEDYVIDIMIDKGASARSVQINLNPFTLIGAT
                     TRAGLLTSPLRARFGISCHLDYYTKDVLTNIVLRSAKILDIEIDHESAGEIACRSRGT
                     PRIANALLKRVRDFAQIKGKGRIDLDIAKYALAALNIDSNGLDFIDHKLLLAIIEKFN
                     GGPVGLNTIATAIGEDAGTIEEVYEPFLIQEGFLMRTPRGREVTPLAYKALGIKRKYF
                     GESDLFSEV"
     gene            complement(23793..24725)
                     /gene="nadA"
                     /locus_tag="PK252_10550"
     CDS             complement(23793..24725)
                     /gene="nadA"
                     /locus_tag="PK252_10550"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT15189.1"
                     /translation="MDNNELISQINVLKRQKNAVILAHYYQVPEVQDIADFIGDSLAL
                     AQYAEKTEADVIVLAGVYFMAETAKIINPKKKVLIPDPEAGCSLADSCNPKDFAGFKK
                     KYPEAVVVSYVNTSAEIKALTDIVCTSSNAVKVVNSIPKGKQIIFAPDRNLGNYLSNV
                     TGREMIIWDGACHVHEEFSLEKIVKLKDENEDARLICHPECKKPIQILSDFIGSTAAM
                     LDYVQKSSHKKFIVATEPGILHQMQKSCPDKVLIPAPPIDSTCGCNDCKFMKLITLEK
                     IYRSLKEDIFEVNIDNELADKARMSIERMLNLSK"
BASE COUNT         8750 a         4983 c         4043 g         7441 t
ORIGIN      
        1 aaatctgaaa tctgaaatct gaaatctgaa atctgaaatc tgaaatctga aatcttgtag
       61 catttttttt ggtaatcata aaatggtttt taaaggttaa taataatatt atcaagtatt
      121 aatgtcacta aagataaaaa agtaacccgt aactatcaag ttttttgcta tttaaaatgc
      181 aaagtgttta tataaaaatc ggttcaaacc ttgcgtgatg ttttgcctta tcacgttaag
      241 acaacctgat aacaaactca agtaagaaga aataatctca attacaagtt tttcatctca
      301 aaataaccgg gatttatttt taaaaagcct tcgaatgctt tatttgagtt tatttactac
      361 ctttgcccca tcaaacacac tttatgttaa atagtataat tgactaaatc ggtatcagtt
      421 gcaacgtaag ccaggcagat aaacaatttt attggttttg gcatactgaa cagttgctcc
      481 ctgatagtcc gatcccgccg tactgattta ctttggttac atctgtcaac caatagtaat
      541 cgttttgttt taaccaaaaa tttataaccg ggtaaagttc aacaatggaa aacttatgcc
      601 caaaaaacat ttagtcatga ttacaaataa caaacagtgg tacgaacaat tattcgaaaa
      661 tttttccaat caatacgaaa acgaagactt caccaaaggc acaataggcg aagttgattt
      721 tttggaacag gaattcggtt ttaacaaaaa tgccaaaatt ctcgacgttg gatgcggcac
      781 aggccgacat tccatcgaat tggccaaaag gggctatcag cctgaaggtt tcgacctgtc
      841 ggaaaaccaa ttggcaaaag caagagaaaa cgcagctaag gcagccgttg tgattaactt
      901 cttccaggca gatgcacgta cttttgactt taaagaaaag tacgattacg ccatcatgat
      961 gtgcgaaggg tcttttcccc tgatggagac agatgaaatg aattttgaaa tcctttcaaa
     1021 cctttacaag tcggtaaaac caggaggcaa aattattttc accacgttaa acgggctctt
     1081 tccgttattt cattcggtaa aagactttat caacgataat tcgctttgtc aaagctttgg
     1141 caacaccttc gacttgatgg cctttcgtga ttattcaaac tacacgttca aagatgacgc
     1201 gggagtagag cacacaataa aatgtaacga acgttattac gtaccttcgg agattacgtg
     1261 gttgttgaaa tcactggggt tcaaaaaagt aaatattttt ggggcccatt tgggcaactt
     1321 cagccgttcg cacgccctga ctacagaaga ttatgaaatg ttagtcatcg cagagaaacc
     1381 ggctaagtaa taataaaacg ctttgcattt aaaaaatgca aagcgtttta tctttgtttt
     1441 gaagtggtta tcaaccaaaa atccacttat acaacggaaa gacaacagaa atgatgggta
     1501 tatcattata aaccttgtag ccttcatcaa atctattttt gacatcctca tattgcctca
     1561 ctaaaggtaa ataggcttgt ttacctaata tagcattcaa aagctgcatc aataaacaat
     1621 caaaaagcac aatacaaaca accgtcaggt aaataaaatc actggtggta atattcaagg
     1681 gcgaaaacga aaaaccagaa attataataa ggggcaaaag ccacaccaac acacgaatga
     1741 caaaaccaaa gctatacaaa tagctaacac tggactttgt gcgggtaaac aaattgacta
     1801 aggccgctat tgtgccacct gagataattc cgaaaataaa acgccacaca aggctatcaa
     1861 cgggcaaaag aaacaaacaa agcaagatag acagaacttt tatgccaatg ttcaatgtat
     1921 gaataataag ggctttttgc gacctcggaa caagaacctg aacattttca gtaacataat
     1981 tagcattatg ttgacgaaat ccttttgtac taaggcgtat atctttcaat tttatcaaag
     2041 tgagcacaaa cggtttatga ttgccaatat gtcccttaca ataaaaacat tctttttcac
     2101 aagctgttgt ttcatagaaa attctcttta ttcttttaaa gttatagtaa aagtttcccg
     2161 gcataaaaaa gcctttacgc ttgtctttca tgtaccacaa caagatagca gcaaaaggcc
     2221 ctgtcaataa taatatgaaa actgcatacc aaaccgtatg aaatgcaaca acaaaaggca
     2281 ctacaataag tgcagccgtc aatggcaaca ataaaagcct gataaatatg gtaaataaaa
     2341 gcccaaggtc tttgaggccg cttttctgaa aagcttcatt ttggcggaca aactcctcat
     2401 accgcatttg agcctgtctg ctggctcgct cgcgtatttc ttccctgaac ctttcatact
     2461 cagcttcttc agcagccttt gaagcagcgg tttcgtatgg ttgttctttt gctaaacgct
     2521 tatttcgctg actaagcaga atttcataag cctcagatat ttcaataaat ttctcatgcg
     2581 catccttcga tttgtttacg tcaggatggt actctttggc cagtttccgg taggcttttc
     2641 gaatatcttc ttcagaggca ttttctgata attgtaatat tttaaaatat ttattcacaa
     2701 taatataaca ttatttagtt atacttttac atgaaaaagt aaatctttct atcctgaaat
     2761 ttataaattt agtttaaata tattaaagac aaacgctttg ctttttggaa aaacaaagcg
     2821 tttaggtaat aacattcgca gaccttccag gttttttaaa ccggaaaggt ttcttgctca
     2881 agaatgaatc gaaacaaaaa aggcccacat attgcgggcc tttgatttga taaagtatgt
     2941 aattaaaatt tgtacataac cccaacatta agaaataatt ggtcagcatc accaaaagta
     3001 tatcccactt gtccggtaag atcaatattg tcagtaatac cgaaagctcc accagcgcca
     3061 aggttcaaac ctatctctga ttcgtcatca gaaatggaag tgccaaaaag ttccatctca
     3121 accttaccaa tactatagtt caaaccagct aacccataaa cagtcatgtt tttttcagca
     3181 ataaaattgt aatggccatt taaactaata gtagttagtt gaaatttaat accatcaaca
     3241 ggttcacttg gaaaatagta agataaagaa acttctgcat cgattttttc aacgatttga
     3301 gccaatgcat tgatattaat accaaagccc attttatcat cgccttcatc actaatagca
     3361 gctttgcttc ctaatgctaa tccggcacct atgcctttaa attgaaactg agaaaatgca
     3421 tttacgctaa atgcaagaat tagtgctaaa aataaaaatt tttttttaat gattaattgg
     3481 ttaataaata tctactcgta agcttttcga catccgctac gtactctttt agggattttc
     3541 cgttttctcc gtccatagat ttaggtcatc tacttatatc attggttttt ttggtttcag
     3601 aaaattttat aaactcaaat atgagaaatt ttattttaaa aacaccatag tgttaatata
     3661 tttctcttaa acaatgcaat aataaaagaa acgggggaca aaaaaatccc ggtttacaag
     3721 gattttcact caaaaagcac ctattgaaaa tagaacttgc acgcgtttgt aacacgcgca
     3781 atgctattaa gttaaggaat tttaatgtct atagggtttt caaataaagt tttacgaact
     3841 ttgttgaaaa ctctttgctt gctccagagg agcaacctct ttatagaaaa aacacaatct
     3901 aagatttaga agcgcgccaa tatagctgtt ataaaatgta gaaacctaat ttggcgcgca
     3961 atagaagaaa ctatttatag tttagttttg ttgcgcacca tacagctgtt gttcatttaa
     4021 ttatttttgt gcttggtgtg cttattttta tttcaacttg tatcctataa acaggacact
     4081 cgccaagcga gttaaaattc cactagttcc agcaaactca cgtttaatgt tattaagtta
     4141 aggaaaatat aaacgccgac aggtctcttc aagaagctgt ctggttataa gttttgtttg
     4201 acctgaccgc atttgcaaaa tcggttagtc ataaacaaca ttgaataatt ttttcgtatt
     4261 tatcaattca aatcattgaa tgaaataact ttacgacttc aaaaaccaac taatacatat
     4321 tcaatgaaaa aagttttaat tataatgctt gtaatggcat cgtgtatgca attgcaaagc
     4381 caggacaggt ttatcaggcg caatgggcaa gacctgttcc tgaacggcat gaatttggca
     4441 tggatgagct atgccaatga cttaggggct tttaatgaag ccgcatttat caaggcagta
     4501 acctctatcc gcgaatcagg cggaaacacc atccgctggt ggctacatgt taatgggcag
     4561 aacagcccgc agtttacagg tgataaagtc agcggcataa gccagacaaa cctcgcaaac
     4621 ctcaaacggg cattggacat agcagccgaa aatggcgttg tccttgactt atgtttatgg
     4681 tctttcggga tgctggacaa aacttatact gccgcagttc tcgacagaaa tactaaactg
     4741 cttaccgata cggcatatac caatgcatac atacgcaatg cattattgcc catggtacga
     4801 tatgtcaaaa accatccggg attgctttgc tgggaggtat tcaacgaacc cgaaggcatg
     4861 tcaaccgaat acggatggtc agattaccaa catgtaaata tgtcgtatat ccaaaagttt
     4921 gtaaaccttg tagccggtgc aatacacaga gaagactcaa cattaaaagt atcaaacggg
     4981 actgtgacgc tccgaacagc cacagacatg gacgggttta ccaattttta ccgtccggac
     5041 aggttgataa aagccggggg cgattcatta ggcacactgg acttttacat ggtacactat
     5101 tacacagcaa acggaacgca atattcgccg ttcaggaaac cggccagcta ctggaaactc
     5161 gataaaccgc tggtcatagg tgaattttcg gcaaaaggga tcaccgaagc cggcgctccc
     5221 gcacaaaccc ctgaagaact atacaattgg gcttatgacc aaggctatgc cggggcacta
     5281 tcctggacct acaccaacca cgatggtaat ggcggattgc ccgattgtgg cgctgccatg
     5341 ctgaaaatag cttttaacca cagcgaccat gttattctga aagacgccaa gcccttcaac
     5401 ttcaacccct accaaaaaaa accgctttat ataggctgga aatataaagg cacaaaagat
     5461 accatacgaa tatcgccgct tttagcttat attactgata aagaagacag ttcgctgctt
     5521 acattcacag tgaaaagcac aacaaacccg gttgcagcaa atataaaaag caatgatacg
     5581 ttagagctta tttcatcaga ggctaccgaa gcaggggttt atacaacgtt aataacagca
     5641 acagatacgg gaaataagtc tataacttta ccagtattta tgagcgttat agatacaaca
     5701 tccgatgatg ttcttctaaa caggccactg tatgtttcga gcattgaaag cacgcaatat
     5761 tacggacatt tcgccaacga caacagcgat actacccgct tttcaacaga atacgccgac
     5821 aaccaaagta tgttatttgt ccttgacaaa gaatacaccc tgcaaaggat gttgatgaaa
     5881 tgggaagccg cttatggcaa agcatacagc atcgaattgt ccacagacag cgccaattgg
     5941 acaaaggttt attcatgcac caatgaaaac gggggttatg acaaaattgt tttttcgcca
     6001 acaaaagcaa aatatgtaaa agtaaactta gaaaaacgtg gcacacaatg gggcttttca
     6061 ttattcacca ttcaggcttt tgaagatgcc atagccaaca acgccccata ctttttacca
     6121 aaaataagtg acaccacatt ttacacccgt accaggttca cgttgaattt gtcgcaatca
     6181 gccaaagaca gcgatttggg agaccgcatc aaataccgtt tagctttgtt gaacaatgca
     6241 aacttaccaa cttttatcac ttttaatgac tcaacctatg tattgaccgg aaggcctacc
     6301 agcacagaca ccacagtgtt gacatttgta ataacagcaa cagatatcga aggagcagag
     6361 gctactgata ctttttctgt aaaaattacc cagaaaacag ccattgaaaa tattgcccgg
     6421 aacaatgtca ggctgtatcc aaacccggtt tcagacaagc tcatcattga aaatgctgaa
     6481 aacattgatt gcattttgat attcagcaca gacggaaagc tattgaacaa atacgctgtc
     6541 aaaacttccg ttttgaatgg taaatatgaa ctttatttgg ggcaactgaa tgcaggtaat
     6601 tatttagtac atttgcaatc gaaatcgggc aatacaatag ccccaataac agtaataaaa
     6661 taatcggcca tgcatgaact ttcgatagca caatctgtca ttgataccat caaaaaaaat
     6721 gtaccatcgg atttgctgcc gaaagcagat aaagtaaagc tgcaaattgg cacgctgtca
     6781 ggaatagagc tggatgcact gacctattca tttgggatac tggtgcagaa cacagcattt
     6841 ccacagctcg caatggaaat tgacctcaag gaaggaaaag gccaatgcca ggcttgcgga
     6901 accgaattca catacaacaa atatggaatc ccctgccctc agtgcggggg attttctatt
     6961 aaaataattg atggcaaaca gatgaaaatc atcaacataa ccatgaaata agtgtttaga
     7021 gaagtatgtc gataaatttc gttatgttta catgatttta atgctatgac aaacttaagt
     7081 tccatacgcc ttccggtcga aaaagaaatc gctgcatttg aaaagcattt ctcagatagc
     7141 cttgatactc aaatcccact gttggatacc attaccaact atttgttacg ccaaaaagga
     7201 aagcaaataa ggccgctgtt tgtcttcctc acggccaaga cattggcaga gcctacagaa
     7261 tcaacgtttc atgctgcaac attcatagaa ctcttacata ccgccacttt gatacacgat
     7321 gatgtagtag atgagtcata tcagcgtcgc aaccacttta gcatcaacgc tatatggaaa
     7381 agcaaggtag ctgtgctcgt gggcgattac tttttgtcca aaggcctttt actttcagta
     7441 aaacataaag agtttgaatt gctcgaagca atttcacaag ccgttcgtga gatgagcgaa
     7501 ggcgagctga tgcagataca gacttctaaa aagtccattt taaaagttga aaattatttt
     7561 gaaatcatcc gcaaaaaaac agcttcactg attgcctcat gttgcgcctg tggcgctata
     7621 tctgtcaaag cagacgaaca gaccattgaa aaaatgcgcc agtttggctt ttacattggt
     7681 tctgctttcc aaatcaaaga cgacctgctc gattaccagg aaaaagacct gattggcaag
     7741 ccaaaaggca atgatatcaa ggaaaagaaa tataccttgc cttttatatt ggccgttcaa
     7801 aacaatcaga aagaaaaaag ccgcatttta aggcttttca accaatcaca taaacccaca
     7861 gaaaccattg aggaaatcag caaattcgtg atagataaca acggcattgc agattctatc
     7921 aagtacatga acgaattccg ctcaaaagct ttagatatcc tttcggaatt ccctgacaat
     7981 gaagcaaaaa agtcgctgat actattggtg gattatatca ttgagcggaa gcattaagat
     8041 aatccttcag aataatccct tcgttcggct aaggctctgc cttagtcgta agtatttcat
     8101 caggctaaag cctgtaaaga taagaacagc tgaggccgaa gcctcagccg aacgagagaa
     8161 taattttatc tctaaacctc ccatccctcc ttaaaaagga gggctaacaa agttgtgtaa
     8221 agctaaaaag cactgctctg ttaaagcccc tttttaagag cctgtcccgt cttaggcggg
     8281 aggtttgggg ttaataagaa ccttagcttg accgctatac gaacagaaat acacaactgc
     8341 aaacaactca aaaaataatt gtaaacgatt cgttattggt gtaattttgc accaaaacaa
     8401 acttatgaca agacaatgca tatcgttcac aaaaccaaat gatgaatggc taaaactaca
     8461 agtggacagc aaagaatatt caagtaaaag tgaagtggtc aacgatttga ttagacaagc
     8521 tcgtaaacag caggtgcaga ttgatttgat cagagcaaaa ctagataagt ctgaggcaag
     8581 cggatttacc gatttatcaa aagaacaaat tttaaaacaa tcaaaggctt cacttaatgg
     8641 ttaaatataa actgagcaat gaggccaagg aagatctgat aagaattcat cattacggag
     8701 ttgaaaagtt tgaaattact caagctgatg aatattttga tgcatttttt gaatatttcg
     8761 acattattgc ccagcggcca ctttcatttg aatctgtcga ttatattaaa aaaggatata
     8821 gacgatgcgt ctgcggggta gatagtatct atttccgaat agaatatgga accgtagaaa
     8881 tcgttcagcg tagcttgtcg ttaaaagcgg ttcattacta aaaataagta tttacagcaa
     8941 attcaaattg attaataaag ctcaaaaatc aaaataaatg agcttattat taaaaaaaca
     9001 tgagctataa aggtcaaagt tttattctcc tgctgcttct gattttggta acagattttc
     9061 gtgagctgac agcttttagc acttcaattc tttgcggggt aatacgataa ataatcaaat
     9121 aagaaccgat aataatattt cggtacattt tactttttgt aggtatctgt cggcattcgg
     9181 ggtaagaaag ataagaagta ctcaagcacc gaatcatgga tagaagctca gacttaaaag
     9241 attctgcaag atttttccca aaagtttcat acccataaag ataaatctcc tgaatgtcat
     9301 ttgctgcttc aggagaaatg attacaagcc tttttggcgt atccattcct caaatttgtt
     9361 ttcaaattct tcaatagtca taaatgggcc ttcttccgct tttttaatag cagccataaa
     9421 ctcctcatga gtaaccttac caggaacagc ccagtttatt cgctttgatt cttttgtttt
     9481 caccgacttc ataatcagta ttttttgtaa aggtacaaaa gatttgtggt gaatatttat
     9541 ctacaaatta attatctttc cccagatgct ctctcagtat tttcgcctta gccatcccaa
     9601 tcacctcagc caattcttcg agagaagcct gatgaatcgc ttcagctgat ttaaacttaa
     9661 tgaaaagttc attaacggtc ttctccccta ttcctttaat attttcaaac tcagaattca
     9721 tccaggtatt ggaacgttta ttgcgatgaa aagtaatccc aaagcggtgg gcttcatttc
     9781 ggatatgttg aattaccttt aaaacagggg atttcttgtc cacatacaac ggaatactat
     9841 cgccaggttt gaatatctct tccaaacgtt tggcaatact aatgatttca atctttccaa
     9901 ctaaattcaa cttgtcaagg ctttcaagtg cagcccctaa ctggccttta cctccatcga
     9961 taacgataag gtcaggcagt ggcgcatttt catccagtaa ccttttgtaa cgcctgtaaa
    10021 caatttcctt catcgaagca aaatcgtcaa tgccagtcac agtctttata ttgaaatgcc
    10081 gataatcttt cactgaaggt tttccattct taaataccac acaggaagcc acagggtttg
    10141 taccttgcag gtttgagttg tcaaaacatt ccatgtgcag cggaagtttt tcaaggcaca
    10201 aaacatcctt catttgctgg actataatat tggccttagc tccttttttc tcagccatct
    10261 cagaagattg cttcagcttt tcctgcagga acatttttga attccgcatg gacagttcaa
    10321 gcaacttaag cttatcgcct gtctttggaa tagtgtaagt aattccttgc atttgcacat
    10381 ccggcatttg cgaaacaaca atctccctgg cattgctcga aagcttttca cgcatctcca
    10441 aaatggccat agacaagatt tcactaaggt cttcgtcaag ttttaatgaa gtaaacatag
    10501 aatgcacctg aacgattgct ccatttaaca cccgcagata attcactacc accattttat
    10561 cagacgaagc caaagaaaat acatcaacat tatgtatcgc agggttaaca atggtcgatt
    10621 tgctgcgata attgtccaaa atagcaatct tttcctttat caggttggca ttttcaaagt
    10681 caaggtcttt ggcaaaagca ttcatcattt gtttcagatg ctggctcacc tggttcaggt
    10741 tccctttcaa aatatccctg atggtctgga tattttccat atagtcatcc agcgactgct
    10801 tggcaataca aggccccaaa caattgccca tgtgaaattc cagacaaggc ttgtacattt
    10861 ttctggtaat tttgtccaaa gacaaatcca gcttgcaggt acgtaaaggg aacaattgct
    10921 taatcaacga aagcaatgtt ttgaccatta ctgtagaagt aaatggccca aaatattcag
    10981 aaccatcctt tatcaattgc cgcgtataga ctatacgggg aaaagtttca cgcttgatgg
    11041 caatccaggg aaaagactta tcgtccttga gcaaaatatt gtaccgcggt tgatattctt
    11101 ttatcaggtt attctccaga agaagggcat ctgactcaga agccacaaca ataaaccgta
    11161 tttcatgaat attgcggaca aggatacgaa gcttggcatt atcatgatgc tctttggcaa
    11221 aataggaaga tacccttttc tttaaatcct tagcctttcc cacataaata atcttatggt
    11281 cagcattaaa atactgataa accccgggca aatgaggcaa attttcaagc gtcttttgta
    11341 tttcggtcga tagttccatg ttcagcaaaa taaatcaaca agtgaatatt ctgcaatttt
    11401 aaataagcct ttatccgtca attttaatgt tggaataacc gataaagcta aaaatgaaag
    11461 gttcataata gggctgtgca atgaacagcc tgcttctgca attttctttt ccaagcggtt
    11521 aaaagcttca gcaacctctt cagccggcat gccagtcata aggcctccat atttcaaagg
    11581 cagaaattct ttttcttgtt cagaaaaaaa tgccagtcct cctttgtttt caataatagt
    11641 attgacagca ccaacaatat cttcatcacg aaccccaact gcaataatat tatgcgaatc
    11701 atgagcaaca ctcgatgcta ttgccccgta tttcagctta aaacccttga caaacgcaat
    11761 gaccggaggt ttattttcat acctgttgca aacaacaagt ttaagtatat cctctgaaac
    11821 agttgattca aaattattta aagcatcatc aggccattga taaagcaatt cattggtgat
    11881 aatctgtttg tcgattacct ctattacccg catagacata cttttctttg ccataagcaa
    11941 atcgtcaaca gctattttct tatcccgaaa tggaaataca aaacttcctg gtaaagactt
    12001 ttgttgaaaa tgcatagatg gtatcccacg aaaataaact tcctgaacat taaaatcatt
    12061 caaattgtcc acaacaataa aatcagcctt atcccctacc ctcaaaagac cgcaatccat
    12121 tttataatgc tgtacaggat tgatacaagc caccttcaaa atatcaaaaa tatcgtaacc
    12181 atgcccgaca agccatttca cagagcttcg tatgccaccc tccaacaaat ggtcagcatg
    12241 gatatcatcc gaacaaagca tcaaatcatc agcacaatta gtaaacaatg gggccagatc
    12301 tgccagattt tgggcagcac tcccattgcg gataagtatt ttcatgccat acttgatttt
    12361 ctccagggct tcatgcacat tggtacattc atggtcagta cttatcccgg cattagcata
    12421 ttttatcaat tcttgtcccg aaaggccggg agcatgacca tcaatcggga atccatattt
    12481 tttgctcaaa gacaacttag ccaaaacctc tctatcctca ttaacaacgc cgacaaagtt
    12541 catcatctca gccaggaaaa aagcccgttt tgttgaaaac aaatgctcaa tttccttgct
    12601 tgaaagaact gcccctgacg aatcaaaagg agaagctggc acacatgaag gagcaccaaa
    12661 caaaaaatga aaaggcacat cctttgtaca ttgcaacatg aaatcaattc cattaacccc
    12721 ggctacattg gcaatttcat gagggtcgca aaccgctcca accaccccat ttttcaaagc
    12781 cgctttggca aattcattgg gcgtgagcaa agagctttcg atatgcacgt gcgagtcgat
    12841 aaaacctgga agaatataat tggaataaat attatcctct tcaacaatat ctttgatatg
    12901 acctttttca atcatcaaag tgccggaaaa tattctcttg gaaaatacat caacaatgtt
    12961 tcctgaaatt ttcatggtgc agatttttat ttaaaaataa tcaaaactat ttaactacct
    13021 caaaccctca ttgccatcaa cataagaaat taatcttagt ttataacatc cacccctacc
    13081 cccgccagcg tgggacaggt cgtatttatt ttcttctaaa ctacattaca tgcaataaac
    13141 gattgttgtt tccataaaat tgttgataaa tactatccgc cgcaggctga ggcaaaaata
    13201 gggggggtgg atgttgaatg ctgaaacttt attcatcctc atagcttgac agttatgccc
    13261 aaagaaatac tacctcaaca ctaagcctca atcaagcatt ttttgaaaaa ttggaaaaac
    13321 attcgttcat gttccacgtg gaacaaaatc acaacttact atttatcaac agtttcaaag
    13381 ttgccaattg aacaaatttc tatcttcagc cccaatgttc cacgtggaac atttacctgc
    13441 caatatgaat aaggattaca ttgatgtcgg atggagagat gccagggatg cgagaagctt
    13501 gtccaatggt ctgcggtgca tactttttaa atttttgacg agcttcaata gtaatgttca
    13561 atcgttcaaa atcaaaatta gcagggatct ttattccttc taattttttt attttgttgg
    13621 caatattttg ctcacggtcg atatatccct gatacctcaa agcaatttct atttcaaaaa
    13681 gaaattgctc gtcaaggcta aaattgtatt tcccgagatg atgaagatgc tgaaataagc
    13741 ttaataacgt aaattgcgga cgcatagcaa tttctcgtat tttaacagat tgcaaaattg
    13801 tagtacatcc gtattgcaaa agaaagggat tgatctcgtc aggaagaaca gagtttgaag
    13861 ctatatattt caaaactatt tctttttgtt cacttttatt aagccaatct ttataccttt
    13921 tttctgaggc caaaccaatt ttaaaacctt tttttgtcaa acgatcatca gcattatcct
    13981 gacgtagtaa aatacgaaat tcggcacgtg aagtaaacat gcggtaaggt tcatcaacac
    14041 ctttagtaac caaatcatct attagcaccc caatataagc ttcatctcgg cttaatataa
    14101 acggttcttt cccattaatt tttaaatggg cattaatccc ggccattaaa ccctgacctg
    14161 cggcttcttc atatcctgta gtaccattga tttgacccgc aaaatataag ttttcaatat
    14221 atttagtttc taaagtgttt ttcaattgag ttggaggata ataatcatac tctatggcat
    14281 atcccggacg aaatatttta acattttcca gtccgaccat ttttcgtaat gccatatatt
    14341 gaatatccca aggcagtgaa gaagaaaaac catttaaata atattcatga gtgtcttcac
    14401 cttctggttc caaaaacaat tgatgcgcgt ttttttcagc aaaagtcaca attttatctt
    14461 caatactagg gcaataacga gggccacgcc cttttatgat acccccgaaa agaggagaat
    14521 catgaaaacc tgtccgcaaa atattgtgca cctcctcatt ggtattgaca atataacaag
    14581 gtaatttttt taaagcaggt ttaacttcat gtaaataact aaatttacct tcttcagatt
    14641 cctgaatttg aagttttgaa aagtcaacag tacggccatc aatacgagca ggtgtcccgg
    14701 ttttcattcg gccaacttca aaacctgcta actttaaaca ttcagaaata cccatactgg
    14761 ccggttcaga aacacgacct cctggaacgt gattgcgacc cacatgaata agcccgtttt
    14821 caaaagtacc aatagttaaa ataatagcct tggcaaaaaa tttaacccca atagttgttg
    14881 taaccccaat agcaacccca ttttcaataa tcaggccatt cacagaatct tgccaaaaaa
    14941 acaaattagg aattgcttcc aatgctttac gccattcgac cgaaaaagcc acacggtcgc
    15001 attgtgcacg aggactccac atagcagggc ctttagacct gttgagcata cgaaactgaa
    15061 tggtactttt gtcagtaatg ataccagtta aacctccgag ggcatcaatt tcgcgaacaa
    15121 tctgtccttt ggctatgcca ccaatggcag gattgcaaga catttgacca aatttggtca
    15181 tatccataga tataagcaac gtactggaac ccatattggc agcagcagca gcagcttcac
    15241 aaccagcatg gccggcacca acaactatta catcataact ttcaatcata aagctttctt
    15301 aaataaaagt cttctttcct tctcatcaaa tttctttccc tgatactttt atctttatat
    15361 ccggctaaat gtaaaacccc atgaaccata accctgtaaa tttctttttg ataagggata
    15421 gcatttttat gggcataata ttttactgta tgtaacccaa taaaaatatc cccaccaatt
    15481 tcattcattt gagaataatc aaacgtaata acatcagtag gataatgatg gttcaaatat
    15541 ttaatattaa gaagcctgat atttctatca ggcataaata tgaaatttat attttcaatt
    15601 ttacaatgtt caatacgttc aatcattctg aatgtagaga acaatgcttg gaaggaaatg
    15661 tgaaacccag gtaaatcctt gtaaaaaaaa ttaatggaca ctcaattaat cttaaattta
    15721 aggaccacta aagccccatt attaaaaaat tcaatgttat cggataaacg ggtcataaga
    15781 aaaacaccac gcccacttac attttcaata ttttcgggag cagttgggtc aggaatattt
    15841 ttataatcaa aacccttgcc ctgatctttt atagaaattt caagaatgtt attctcacat
    15901 ttggcattaa tatttaccat aagttgttca ttcagcttat tgccatgtat aatagcatta
    15961 tttgcccctt caagggtagc aataagtaaa tttccatata actcagaatt taatttgcat
    16021 tctgatgaaa tttcatctac aaatttttct acctgacgga gattttcaat ttttgaacta
    16081 atatctatat tcttttccat ctatactcat tttaaatgga ttatactcaa caaagttaat
    16141 aaaggttacc aaaattataa tatttatcat taataccaaa ttttatttaa cgagaatatg
    16201 accagctaat tacagatttg atgtaaattt taaatataca gttacaacgc aggttttgaa
    16261 atattattgg ttctttagcc aataaatagg gttttcttta gtaaattctt tccaaatttc
    16321 aaaatgcagg ttagcagcac catttccggc ctcaacacaa ctacaaaaac caatcttttg
    16381 attagtatca acattatcac caactttaac aaccaccgaa ctcaaatttt gatacaccga
    16441 aagataatca ccatgcctga taataatagc agtattagct cctttaatag caaaaatttt
    16501 agataccaca cctttaaaaa tacattttac aggttggtta caatcaaacg aaatatcaat
    16561 accaaaatta ttgattttga ccccgctcaa ataaggatgt ttatgttctc caaaataaga
    16621 ataaacagta ccatttgcga caggccaagg caatttgttt ttgttttttg aaaattcatt
    16681 tgacaacaaa acaacttcct ctgtttgttt tttatccatg ttggcttttt ttcgggcttc
    16741 ttcttcgata atgcgataaa tttcagcctc aatttttttc ttaacaaggt ttaatgtttc
    16801 taattctttt ttgaacttgt taattttctt tttattttca acaattgtgg cagataataa
    16861 gcttttatcc ttttcaagtt ttcgtttttc gtcctctcgt aaagccaaaa cattggcttt
    16921 atcttttcgt accgacaaca aataagaaac agaacgtttc agggtatcaa cctcagtaac
    16981 aatttctttt aacaaaatac ggcgatgctc cgtaaactgg cgaaagtaat ataagcgacg
    17041 ataagcctgg ttaaaagact gagaagataa taaaaacatc aaaaaattat aatctttgaa
    17101 atgaaaatag atttgataga tagtttttgc atattcagct ttaaggcttg atatttcatt
    17161 ttttcggaaa tcaatattac catttaaata aacaatttgt gaatctaaag actcaacctg
    17221 atagttgagg ttttcaataa tttcattacg caaaacaatt ttacggtcaa ttaactcaat
    17281 agcattcata ttgctttggg tagcttcatt agttttacta atcagctttt ctatccggtc
    17341 aatttcttca atttttttta aacgttcttt ctcaaggtct ttctgacgct gaccgctaac
    17401 aaataaagaa aagagactga acaatattat taagaaattc ttcatctttt aatcaataga
    17461 aaccttaatt tcatcagaaa tagtaaattg tttcacttta attcttccgt tgagcaatac
    17521 atacttgtta actaatagtt gtgtaaattt actatttacc tcacacaata aactaacaga
    17581 atacttaaag ttgtcaggat taacaatttt atcaacttta tacggcacat tggcgcttac
    17641 actcagtcca ttaatcagta aatcatccag taaataatat aaaaagcaat catcaaaaag
    17701 tttttggtaa accggggaaa tgtccacaaa cgaactttta atatgaactg aatctttata
    17761 agcaataata tcagcaactt taactgcccc atcgatataa acctttaaat aaagaacaga
    17821 atcacttttg cgaaaagcaa ctgaataaag ctctttatta aaattcacgg taacagtgcc
    17881 agaattagtc gtcgaaccaa cagacttttt ggacacagaa catgaaaaca aacaaataca
    17941 gcaggacaat atgcaaaata ccttgttcaa agctatttac atttaatatc tgaaactaat
    18001 ttatcctgaa atttctgtcc taaatttttt ttcaaagctt ttaaagcttt ggcagccaaa
    18061 cccttttcat tagtacaaac cgaaatagca tacaaatgca gataaacatc tttagaaaaa
    18121 tacttcgaat actttattgt ttctttaata taaaaaaggg catctgtata attaccgagt
    18181 ttaaaacaaa tccaggcata agtatcaagg aaagtcgggt ttttaggttc agcagtaata
    18241 gtcaaacgcg acatttcagc agctttgatc aaatcaactc ctttttcagc caaataataa
    18301 gccaagttgt tataaagcaa attatcaggt ttgtattttt tgaaaacttc agcataaaca
    18361 acaatagccg agtcaaggtt gccagacata taatatgaat cgcctaaacc caataagaaa
    18421 ttgcgctgta actggtcatt atcaaacttc gtattttgta aaccattaag ataaatattt
    18481 acagccaatt tataattttc tttactataa gcagcaatag cagccatata caccataaaa
    18541 gcactcacac ctactgtagc ttcggcattt ttataatacc ataaagtact gtcaaattgg
    18601 gacgaattat tgaaataata aagcaagcgc tcccaggtac ggagttcatt tttatttcca
    18661 ttgtacaagg ccttagtaca catcaaagct gatttaaaat tgcggtttaa ttcataaaaa
    18721 tcggcaaaaa catcagcaaa accaacatac aagtttacat tgacagactg taaaaccttt
    18781 tcaataaaaa aatacaattt aggttcacgc tgagatataa aagaaagttt agaaaataca
    18841 tctttcctaa actcaatact taacgagtca ttagtgatag ttgaatataa tttctgctga
    18901 aaagcctgag aatttttggt ttgaaacaaa aaataaatat atcggtacaa aatgtatgaa
    18961 tttaaagaat ctaacatcaa agcctcagca tagtattttt cggccaaaga cttagacttc
    19021 atcgaaaaat acaaatcacc atacttaacc gctatttgtg cgtcaaaaga atcaattttg
    19081 acaagtttgt ttaaaatagc tatcccctct ttaggtttat ccatctcatt aaatatatca
    19141 gccaacatca aacctagctc acgtgtaaga ccatattttt ggtacaactc gaaaaaaaca
    19201 ttgttagcct ctttagtatt gccagaaaat ttaagcatca agccataatc aaacataata
    19261 tcagggtctt ctccaaattt atcaagcaaa tatttatatt ttgcagcggc atgaataaaa
    19321 tatccattct ctttcaatac atcagcaaac aacaaggcat accaataatt gctactatct
    19381 ttttcaaaag ctttgctggc gtaaaaaatg gcattacgtt gatctttact tttcatcata
    19441 atatcagcca tctcataata gcaggctaaa caagtagaat cagccctaat taacttatca
    19501 agcgtaacgg cagcattgta ataattacca agttttttat tagcaatagc ttcaataaaa
    19561 agaaattgaa catctttttt attcgcctga gaaaaagcag ttgcacaaat accaaaagtg
    19621 aaaatactta ttaaaagaaa agttattttt ttcatttaca gttatttacc agtatgacca
    19681 aatccaccat gtccacgttc cgaatcagca atttcttcca catcaaccca ctgaacagtt
    19741 tcatgtttag caataaccat ctgacagatg cgatcaccat caaaaatttc aaattcagta
    19801 tttgataggt ttaccaatat aactttaatt tcgccacgat aatcagcatc aatagtcccg
    19861 ggagtattaa gtactgtaac cccatgttta aaggctaaac cactacgcgg acgtatttga
    19921 gcctcataac caacaggcaa ctcaataaac aaaccagtag ggaccaaaac acgttccaac
    19981 ggtttcaact tcaaaggcgt atcaatgttt gcacacaaat ccaagccggc gctatgagcc
    20041 gtttgatatt gtggcaaagc atgttttgat ttattaatca ctttaacctt catattattt
    20101 aattattaat tttttcaagt ttttaacgta attccaagta acaagccata agttttcgga
    20161 tttcaatatc caataaacaa aaatgaccag caataaatta tttactacaa ttaaaaatga
    20221 taaatgtaaa ctatttaaaa acaaaattat aaacaatcct actaaaaaga ttatcaaata
    20281 tttacctatt cgttttacat cataaggaat agagtaattt tttgttccaa accaataaca
    20341 caataaaacc atgagtaaat aagaaataaa gcgattataa gcagctgcct gatatccata
    20401 cacaggaaca aggaaatagt tcaaaattat tgttaataag caaccaaaaa caatgaaata
    20461 aacgccatac atggttttcc ccgaaagctt ataccaaata gaaagattga cataaacacc
    20521 caccaaaaca tttgcaccaa gcacaacagg gacaatagaa agaccagacc aatatttttc
    20581 accaatgtaa tatttaaaaa tagaaatatt agccatagtg aaaagcacta caactaagca
    20641 aaccaggatg aaaaatttca ttacctgagc caacgtatct tttgacgatt tatcttcctg
    20701 acgtttaaag aaaaaaggct cagcagcgaa acgaaaagtt tgggtaaaaa caactaaaat
    20761 aaccgctatt ttgaggtttg caaagtaaat tcccaaatca gacatcgcat catgccccac
    20821 tggagaaaga tatttaataa actgcctgtc gatattatca ttgatcatac cagccaaacc
    20881 cgcaattaac aatggaaatg cataaaccat catttttttc catagtcgaa agtcaaattt
    20941 aaacttgatt ggaaaatcct taataagagt aagaaataca aataaactag aaataaggtt
    21001 agcaataaaa acatatttta cattcaactc aaaatgaaaa acatgaaatt taccagacat
    21061 ccacggaaga attattagca aaacaacatt taatgcaaca tttacaacaa catttaaaat
    21121 tttaagagta gcaaatttta aagccttatt atcattccgc agttttacaa aaggtatagc
    21181 acaaatagca tcaaaaaata taatcaaaat aaaataaaca atatattcag gattaccaga
    21241 ataatcaata aatgcagcaa tatttttatt aaaaattaaa cctaaaaata aaaacaacga
    21301 agaactaaac agtaaggaaa agaaagccgt ggaataaaca ttcctaaatt cagactggtt
    21361 gctgaattta aaaaaagcag tctccatacc ataagtaaca atgatattta aaattgcaac
    21421 aatagcataa agatcagcaa taacaccata ttcattagga agaaaaatac gtgcataaat
    21481 aggaacaagc aaataactaa ggaaacgggg aataatacta cttaacccat aaacaaaagc
    21541 ttgacctgcg aatttcttta tctggctcaa tcttgtaaaa attagatggc aaaaatataa
    21601 ttacacaaaa gtatcagaaa ataaaatatg ttaaatactt ataacaacca taaacaagaa
    21661 aataaataac tttgtcatgc attttgatta aagaattcaa taaccattat aagatgaaag
    21721 aagctattta ccaacatttg aaagatgtag attcatacac aatcaataat agtgcggaac
    21781 ttgaagaatt tcgtttgaaa tatttgagta aaaaaggcat cttacaacag ttatttgaat
    21841 cattcaggac aataccaaac gaagaaaaaa aagaaattgg aaaacttatc aacgaactga
    21901 aacaaaaagt tgaaggtaaa ttttctgaaa tccaatcaaa acttgaagca gattcaactg
    21961 aaaatacagc aattgattat actcttacac ccgtttcaat gaaattgggc accagacacc
    22021 ctttgtcaca agtacgtaat gaaattacct caatattcaa aaccctggga tttaccattt
    22081 cagaaggtcc tgaaatagaa gatgactggc atgtattttc ggctttaaac tttgcagaag
    22141 aacatccggc acgcgatatg caagatactt tttttataga aaagaaccct gacatactgc
    22201 ttagaactca tacatcatct gtacaaatca gggcaatgga aacacaaaaa ccaccattgc
    22261 gaatcatcat gccaggcagg gttttcagaa atgaagctat ttcgtaccgt gcacactgta
    22321 tcttccatca aatagaaggc ttatatatcg ataaaaacgt ttcatttgca gaccttaagc
    22381 aaacacttct ctactttgcc aaagaactgt ttggtgcaga taccaagata cgtttaaggc
    22441 catcatactt tccatttacc gaaccatcag cagaaatgga tgtttcatgc tctatttgta
    22501 aaggaaaagg atgtaatgtt tgtaaacatt caggttggtt agaaatactt ggttgtggta
    22561 tggttgaccc taatgttttg gccaattgtg gaattgaccc taatgtttac actggctatg
    22621 cttttggtat gggcatagaa cgcattacaa tgcttaaata cgctgtcaaa gacttaagga
    22681 tgtacttcga gaacgacctg aggtttttga atcaatttta agctctttct aagtcacatt
    22741 taaactccgc tcaatgtgac ttaaacttca ctgaacaaat cgctttcgcc aaagtatttt
    22801 ctctttattc ctaacgcttt gtaagctaat ggcgtaacct cacgcccacg gggagtacgc
    22861 atcaaaaatc cttcctggat aagaaaaggt tcataaactt cttcaatagt accagcatct
    22921 tcacctatag cagtagcaat agtattaagc ccaacaggcc caccattaaa tttttcaata
    22981 atggcaagca acaatttatg gtctataaaa tcaaggccat tagagtcaat attcaatgca
    23041 gccagcgcat atttggcaat atcaaggtct atcctgccct ttcctttaat ttgcgcaaaa
    23101 tccctgacac gttttaataa agcattggca atacgaggtg tcccacgact acggcaagca
    23161 atttcgccgg ctgattcatg atctatttca atatccaaaa ttttagccga acgaagaaca
    23221 atattggtca atacatcttt tgtataataa tctaaatgac aggaaatacc aaaccttgcc
    23281 cttaaaggac tggtaagcaa tccggcacga gtagtagccc caattaaagt aaaagggttc
    23341 aggttaatct gtacagacct ggcactggca cctttatcaa tcataatatc aattacataa
    23401 tcctccatag cagaatataa atattcctct actacaggag acaaacggtg gatttcatcg
    23461 ataaaaagaa catcccccgt ttcaagactg gtaaggatac cagccaaatc acctggttta
    23521 tccaacactg ggccggaagt aattttcaga ttgacattta attcatgggc aataatattg
    23581 gccaaagtag ttttacccaa acccggaggc ccatgaaaca atacatggtc aagtgtttct
    23641 ttacgcaaca aagcagcctg aacaaagact ttcaggttct caataatttt atcctgtccc
    23701 tgaaacttac taaaatgatc aggacggatt tttttatcaa aatctttatc cgttacaaac
    23761 ttttctttat tatactcgaa attattatcc acttattttg aaaggtttaa catccgttca
    23821 atactcattc tggctttgtc agccaattca ttatcaatat tcacttcaaa tatatcttct
    23881 tttaacgaac gataaatctt ttcgagcgta attaacttca taaatttaca atcgttgcac
    23941 ccacaggttg aatctatagg cggggcagga ataagcacct tatccgggca actcttttgc
    24001 atctggtgta aaatacccgg ttcagtggca acaataaact tcttatgact tgacttttga
    24061 acataatcca acatagctgc tgtcgaacct ataaaatctg ataaaatctg aattggcttt
    24121 ttacattcag gatgacaaat cagccgggca tcttcatttt catctttgag ctttacaatc
    24181 ttttccaacg aaaactcttc gtgcacgtga caagcgccat cccaaataat catttcgcgg
    24241 ccagtgacat tgctcagata atttcccaga ttgcgatccg gggcaaaaat aatttgcttg
    24301 ccttttggaa ttgaattaac caccttcact gcattggacg aagtacaaac aatatcggtc
    24361 aaagctttga tttcggcaga agtattgaca tacgaaacta caactgcctc aggatatttt
    24421 ttcttgaacc cggcaaaatc ttttggattg caagaatcag ccaatgaaca accagcttca
    24481 gggtcaggga tcaacacttt cttttttgga ttgataattt ttgccgtttc ggccataaaa
    24541 taaaccccgg ccaacactat aacatctgct tcggtttttt cagcatattg ggccaaagcc
    24601 agactatcgc caataaaatc agcaatatcc tgaacttcag gtacctggta ataatgagca
    24661 aggataacag catttttctg tcttttcaac acattaatct gacttattaa ctcattatta
    24721 tccatattta ttttttttaa ttttttaaac tttaatgatg ttgatattaa ttagctgtta
    24781 acaatgtttg aaagtatttt gcaaaaatat tgcattttac aaaatgaacc ttttaaaaac
    24841 atctattaac aagaaacaag aatactaacc ttctcgaaat cagtactgaa acaaagtaat
    24901 taacaactgt taagagcttt tgtttgttaa aatttagtgg cttattaaca tggtgaaaat
    24961 gtttaataaa cctgctcata gcagtttgat agaatacaat aactatattt tgaaaatgga
    25021 atttaatggt taatagatag ccgaaaacaa aaaaacaagc aaaatggaag aattttctaa
    25081 aaagctatta acaatagttt caacatggag ttaacaagaa aataacagag aattaaaaaa
    25141 aacgccgata ccttccggta ccgaggtagt ataaaaaaga ttgtgtaaac actcataaaa
    25201 gtaaccagat aatgctg
//
LOCUS       DAOSPF010000053        24791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2022_length_24791_cov_5.894243, whole genome shotgun sequence.
ACCESSION   DAOSPF010000053 DAOSPF010000000
VERSION     DAOSPF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_24791_cov_5.894243"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..392
                     /locus_tag="PK252_10555"
     CDS             78..392
                     /locus_tag="PK252_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT15190.1"
                     /translation="MYDAALKIKRYTKDMDFSSFANDDKTIDAVIRNFEIIGEAANRI
                     APDYKFKNPELEWNHLKGFRNRIVHEYFGVDYEIVWSIIENNLDNYIEWIEVLLNKEK
                     SN"
     gene            complement(396..1772)
                     /gene="miaB"
                     /locus_tag="PK252_10560"
     CDS             complement(396..1772)
                     /gene="miaB"
                     /locus_tag="PK252_10560"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT15191.1"
                     /translation="MIEPGQLQVSHQYTKASGNKIYIETYGCQMNFNDSEVVVAIMQK
                     EGFAVTKDITEADLILVNTCSIRDNAEQRVLGRLRELRAIKRKKPALKVGVIGCMAER
                     MKEQLLEKEKAVDLIAGPDAYRDLPVLVAKTGTGMRAANVILSHEETYADINPVRLDE
                     NHVTAFISIMRGCENFCAYCVVPYTRGGERSRDPETIVNEARNLFDAGYREVTLLGQN
                     VNSYLWKSSTGANITFPKLIELVALVDPKLRVRFATSHPKDLSDDLLRTMARYKNICR
                     NIHLPAQSGSNNILQAMKRGYTREWYLQRIAAIKHYMPECTISTDLIAGFCGETEQDH
                     QQTLSLMREVGYDSAFMFKYSQRPGTYAADNLTDDVPEDVKSRRLTEIIDLQNTLSAE
                     NKKKDIGKTFEVLVEAVSKKSKNELYGRTSQNSVVVFPKMNFNPGDYVMVEITGCTQA
                     TLKGKVVE"
     gene            complement(1989..2453)
                     /gene="tnpA"
                     /locus_tag="PK252_10565"
     CDS             complement(1989..2453)
                     /gene="tnpA"
                     /locus_tag="PK252_10565"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOT15192.1"
                     /translation="MANTYTQIHIQVVFSLQNRECIIGKQWKDELYKYITGIIQNHHH
                     KLLTINGMPDHLHIFFGMRPSESISELMQQLKSDSSKWINERKFIRGKFSWQEGYGAF
                     SYGKSQTEQVINYIQTQEIHHKNKTFIEEYEDYMKAFGIEYDQRFIFKPVVY"
     gene            2664..2873
                     /locus_tag="PK252_10570"
     CDS             2664..2873
                     /locus_tag="PK252_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOT15193.1"
                     /translation="MTFSKKMMDGHKWKLFCLMCRFIGWGLLCLLTAGIGFLWLIPYM
                     YVSFARFYDDVRASYVACLSIKKAP"
     gene            complement(2898..4589)
                     /locus_tag="PK252_10575"
     CDS             complement(2898..4589)
                     /locus_tag="PK252_10575"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT15194.1"
                     /translation="MKKIKYILYLAAISLLAGCGEDFLDQKQLYLKTDADFYKTPQDV
                     ADALTGAYSVLPSYGGASNPTVMANLLSDDCFGGGGSGDITFHDLDKFTNSQADNYYP
                     LWQEYYKGILRVNKIIDRIGQAKYDNEADKNQDLGEAYFLRAYFHFTLAKFFGNIPLI
                     TDPAFAVVPQAKPEVLYAQIASDMKKAIELMKTVNVNNIASERAGHATKWAAEGMMAR
                     IFLFYTGYYNKTEMPLTEGGAVTKANVISWLDDCIANSGHDLTPDFRNFWPYSCVNMA
                     MPNSYPYATNNNLSWVGDAGEKDSNGQLKNKETVFAIKYSTHGGWNLPTKVTYSNQVC
                     LYMGLRIQEGLVPWSQGWGAGPVNPQLNQSFEDGDIRKKGSIIDVTDPEEGNVSTKYV
                     WGSNQCMHETGLWNKKYTSLQVKQGASYVGVFTILVPGSNTNNQLWNLQDEIILRFSD
                     ILLMGAELGGPNAQAYFDRVRARVGLPSKPASLENIKEERRHELAFEGVRYFDLLRWH
                     DAEAAFAKVTNIPVKNSGVDEMYDASFRPETGGFLPIPETEISLSSDVLKQNPGW"
     gene            complement(4622..7810)
                     /locus_tag="PK252_10580"
     CDS             complement(4622..7810)
                     /locus_tag="PK252_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15195.1"
                     /translation="MYKTSHKQKAGWRLKSVLLSLLLLFSGAVIFGQERSISGTIVDN
                     SGAPLPGVNVIIKGTTTGTVSDMDGKFTIKADASATLEFKYVGYLSQEIPVGGQTSFS
                     VTMKEDVQELNEVVVIGYGVQKKKLNTGATLNVAGENIQALKTNSPIEALKGITPGVT
                     IIQNSGLPGAGTRVNIRGVGTIGNSKPLYIVDGIAVGDIDYLSPSDIESIDVLKDAAS
                     SAIYGSRAANGVVLVTTKTGAKDMKTVVTYDGYYGVSNIYKKPEMLNAKQYMEIMDEA
                     NYNTDPAGYAKKAYKWATLVPDYDKIMSGEWNGTNWVDEIENKNAVTQSHALNIMGGS
                     KKSTYSLGASYFTEEGVLGKQTNNKYKRINLRMNTTQVIWEKNNNAILTVGENLTFTN
                     TKKPTFRTGNIYWNDLHNMLVTSPILRAYDSVGNYAPPTKWNLEEANPIALMEYNCNS
                     SYNDNNNINASAFAELQPIKGLIFRSAFSGSNWYGSSRSWVPKYYLTSKTFTTRDVVN
                     QSMYEGFTWTWTNTATYNQALGQHNFTFLLGNEMTKNTQNLSLEGKNENSLFQDAEHA
                     YLKNVPVVDASYTTITGKDEYGWGLLSYFGRLSYDYKETYMFTFVLRADGSSNFPEGH
                     RWGTFPSVSAGWLVSNEPFMDWSKDLVSSLKLRASWGQNGNQDMPNGKFQYLAAQTYD
                     NSSDYFFGTDKTARTAGCYPEHVANYDLTWETSEQTDLGLDLYLYNNKLQFTFDYYNK
                     ETKDWLVQAPIVAMGGTNAAYSNGGTISNKGVEFTLRWNDRDGDFKYGVTASIAYNKN
                     RVTQLNSSDSIIHGSKNVLSQGTVECYRAQIDYPIGYFYGYKTDGVFQDSAEVEAFTR
                     GKSTYYTVDKKTGQLVNKIKPGDQRYVDLNGDTIIDEKDKTMIGDPNPDYILGLQLDA
                     SYKGFYFMASANGAYGHQVAMSYRSFVDGPKQNYTTDILKRWHGAGTSNRYPRLLNTA
                     SDKISDIYIEDADFIRISNITFGYDFKYLFKSLPLTEAKLYVAVKNLYTFTNYIGMDP
                     EVGYAPDSWASGVDLGLYPSSQTYMVGLSLKF"
     gene            8306..9025
                     /locus_tag="PK252_10585"
     CDS             8306..9025
                     /locus_tag="PK252_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT15196.1"
                     /translation="MKYLLSLVFVSLFVTSKTDKTTTTMTSEDLAKNIHWIQQAGFKI
                     EAGSQKIYIDPVGINANDNASIVLITHAHGDHFSPADIAKIANENTTIYMPASCNYDG
                     KYKAKTIVKIGNKYIINDSLTIEVVPAYNVVKTKFHQHNDNFVGYIITYNGIKIYHTG
                     DTERIPEMKNITANIILVPLGQTYTMNNVEEAANAVKDVKAEIAIPMHFGLYEGKTSD
                     TIAFKQYLEGAAKVIILEKGK"
     gene            9195..10469
                     /locus_tag="PK252_10590"
     CDS             9195..10469
                     /locus_tag="PK252_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15197.1"
                     /translation="MNLKIFAFVAGLILFNLLHTEAQPKKAVLSGKEFTPHSIFIHYP
                     STEHAWANAQRGNEWMLNLNGSWLYNTSGSTARLTPDRKGFNPQEWQSFQVPSNIFGE
                     QTELDTMIRPKGNKVILRKFFPIPESWYGLQTELVFEGIGPKGVVLVNGMKAANISNN
                     QYGLFIEITPLLRFGKMNSIDIILENVNAASADSLAGLFGNVALYAHPNVAISDYTIQ
                     ATPASPGNEGKIDITLFPKRYLEDIDDKFTIEMYLYDKKRNNLLAPVALKYNPSKKAD
                     SVLFKSMTVKGAQAWTSCQPVYYTLLILLKDKQKQVVEAIKQIVAFRDFRYEGDSIII
                     NGKRDTCMTFKEMDSKLAITGEPFEKDKLYKLVFTSNLQPTDISNIIRIVRKYRNNLQ
                     LAAYELKGFKDDAAQTIKAAIEAEDKTHPVKY"
     gene            complement(10466..10855)
                     /locus_tag="PK252_10595"
     CDS             complement(10466..10855)
                     /locus_tag="PK252_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15198.1"
                     /translation="MNTVLMAGTTIVNLALISYAIAIVTEQRKKLVSNKVLIFITLGV
                     ILDITATALMVIGSSKGAFTAHGLIGYSSLSLMLIDAVLLWIFRKKYGALTYVGKKLH
                     IYSRIAFIWWIVAYITGAVLVALRHSA"
     gene            complement(10852..11865)
                     /locus_tag="PK252_10600"
     CDS             complement(10852..11865)
                     /locus_tag="PK252_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT15199.1"
                     /translation="MSKTKRFLLIFLQYAIFLGLGIILLYFAFRSIDFQVLVDNLKHT
                     RYDLVVLSLFFGFGGMLARAYRWQLMIEPLGYKPRFANTYHALIIGYTANYAFPRIGE
                     VTRCGVLGRTEKRPADALLGTVIAERIFDVLVLFLLTILVILLKIKEFGGFFSQKVFI
                     PLGKKFGWLFDFSIYTWILAGLAVAAGFFLVYAFRNQIRKITLVGKIKKIAKGVFIGF
                     KSILYLKRKQAFFASTLAIWVFFLLMTYICLKAMQPTAHLTMVDALFLLVVGSYGFAA
                     PVQAGIGAYHGMIALALSIYAISWSDGLAYALLSHGAQAVSIIFMGLISMLLLFLKKR
                     QTR"
     gene            complement(11862..12215)
                     /locus_tag="PK252_10605"
     CDS             complement(11862..12215)
                     /locus_tag="PK252_10605"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOT15200.1"
                     /translation="MWIEVLKSKIHQVTVTEANLYYVGSITIDEDLMDAANLFEGEKV
                     QIVNINNGERFETYTIKGERGTGVIALNGPAARKVVVGDVVIVMSYGMIEVEKAKGFK
                     PTVIFPDTRTNKLVQ"
     gene            complement(12335..13243)
                     /locus_tag="PK252_10610"
     CDS             complement(12335..13243)
                     /locus_tag="PK252_10610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15201.1"
                     /translation="MLAPIALFCFNRPVHLKKTLDSLAKNDLARESELYIFSDGARGV
                     KDVLAVDEVKRVIAEARGFKNIHYTFSEVNKGLANSIIDGVSKVFGAHNRIIVLEDDL
                     EFSPNFLRYMNDCLDFYEHNGQIFSISGYCAPITIPADYKEDVFLFYRINSWGWATWK
                     DRWQLVDWKVEDFDTFIRNKKEREAFNMGGADCSNMLLKQQLGQNNSWAIRFCYTSYK
                     FNKLNVFPTVSKVRNIGADGSGTHVRKSRSFETKLDDSNNTIKLTTPPLNGQIIKAYQ
                     RFFKRSLVRKVIDYYEIKRYKFFGKS"
     gene            13424..14122
                     /locus_tag="PK252_10615"
     CDS             13424..14122
                     /locus_tag="PK252_10615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT15202.1"
                     /translation="MPTNNDMLSGSNKLSIFHPRKYILTKVRKELQKVFDKNITKTDG
                     MLLDYGCGDMPYRNYLSSKVEKYIGADIEGNDKADMLILPNGRTPLADNTSDYVISTS
                     VLEHVPDVALYLNETARVLKKEGLIILTTHGIWMYHPHPTDFWRWTSMGLKKVFEDNN
                     FEIVHFQGVGSRAAMGLLLFQDGLYFKLPVFIRNIFSMLMQPFICLFDKVSKKNTTDK
                     DAGFYIVVAKPKKL"
     gene            14119..15219
                     /locus_tag="PK252_10620"
     CDS             14119..15219
                     /locus_tag="PK252_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15203.1"
                     /translation="MSICIAYPNKNLSFSETFISNHLKFIEHKSELTGGYYPYLTIDR
                     NSIFQFPLSIDLLRGGIKNIAPKVYKRLYTLTLSDYLLKTKPSVVLAEYGVTGSMMTD
                     ACKKTSIPLVTHFHGFDAHQYKILEQYKDDYQKMFDYARTIIVVSEDMKKELIQIGAD
                     EHKIVNLPYGVDINKFSGANPENSAPIFIFVGRLTHKKSPINLIKAFKIVADEIDDAR
                     LVIVGDGELKKQTLNEIETLNLSAKVELKGVLSPLQVAEELKKARCFVQHSVRDITGD
                     SEGTPNTILEASGSGLPIISTTHAGIKEAVVHGHTGLLCAEHDYKAMGQNMLTIAKDK
                     QLAAQMGIKAREHISKNYNIETQMEKLRQLLY"
     gene            15238..15987
                     /locus_tag="PK252_10625"
     CDS             15238..15987
                     /locus_tag="PK252_10625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOT15204.1"
                     /translation="MIVRLINKVIKPLKLNITSISSAAPVDIEKDFLEAYQLAKPYTM
                     TSFDRMYAAYKAAEYVVNAKIEGDIVECGVWRGGSSMIMALKLTAMKSFDRDLYMYDT
                     YEGMSEPTKEDVDLSGVHSSERLSKEDKYKGNNVWCYAGLDEVKQNMLKTKYPMDKIK
                     FIKGKVEDTIPGTIPSKISILRLDTDWYSSTKHEMQHLFPLLSKNGILIIDDYGHWQG
                     SRQAVDEYFKEFGIVPFLHRVDYTGRLYVKL"
     gene            16316..17821
                     /locus_tag="PK252_10630"
     CDS             16316..17821
                     /locus_tag="PK252_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15205.1"
                     /translation="MGIVVRQSIKATVVSYLGALVGYINIMFITPLCLSPEIIGFGKV
                     FLDAALMFTFVAQLGLNSALIRFFPYFRDEKNNHGIFGIILVVPLMGLVLLSALVGIF
                     YNPIIGMFQANSALFADNFYYVLPLTFFMMYLVLFETFSSCLLRIVVPKLIREIGLRL
                     LNIAVIVLFYLKVLDFKAYIISIIVIYVIATVLNLYYLKKITNLSIKPDFSGIPKKVF
                     RDLMFYIAFILFVGIGSNIVSKIDVFMISSSLNLSYAGIFSIAFYVTTIIEIPSKAFM
                     QITNPIVAQAAKDNDFKKIGDLYTKSSINQYIVGGFILLLVWANVDNIFKIMPNGELF
                     QSGKYVILLLGLGKIADMVTGINLIVIANSRYYWYQLIFTSYITATTILLNYYLIPRF
                     GINGAGMTSLIAMSTYNLLLLFFLKYKLSLWPFNIKTLTLTIFFLLLFAINYILPVLS
                     NPILDGIYRSIILTAGFVLVIYKGNISDDFNNLISQISLENIKKHIPTKIK"
     gene            17895..18179
                     /locus_tag="PK252_10635"
     CDS             17895..18179
                     /locus_tag="PK252_10635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15206.1"
                     /translation="MSGLSINEAMAFGKPVVCSVCDGTEKVLVRDGKNGYYFESGNSK
                     SLADKIMSIIGHDEVQTSMGKESERIIKEEINIDVVCNNFISALRTIHSS"
     gene            18176..19828
                     /locus_tag="PK252_10640"
     CDS             18176..19828
                     /locus_tag="PK252_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOT15207.1"
                     /translation="MKNLKTSILLVFSFLFLATINSESQHLNFQNEYIIKFVKPTNVK
                     IEYLTTLISIDKVKGDSIVAYVNTKQLKTFQKLNIPFSLIIKEKATLATTSGNIWDWN
                     KYPTLSEYIQMLDSMQQIHPDLCKIITFGNSALGKPIYYARINKDTSIYKPSVLLSST
                     IHGDETGGSVVLTRLIFYLLDNYGKSSTVTNLIDNLDIWINPVFNPDGRFASTMNRSN
                     GNYVDLNRNFPDIFYGSHPDNNDYQPETRSLMNLYSTYNFVLSATLHAGAEVANYPWD
                     ASPSFHADKQWFENVSHEYADTAMKYGPVNYFQTLYPDGVIDGWYWYPVYGGRQDYVT
                     GVCHSRETTLEIDDNKFTPENELETLWQANYRSLLNYINNATKGIHGIVYDKNTNTPI
                     KATIEVVNYDDSLSVVYSDTKTGHFTRLLPVGTYTLKIYAKGYDTLTVENTYFTMNTS
                     RQLTAYLTPSAKNYIDNNFSGQIKVFPNPAMNTLFISGIYDNEIVLKLCIYNTSGVSI
                     PDLSLEKNTHTIDISHIPDGFYFVFIQTSKKVFKSKFIKRTN"
     gene            complement(19885..>24791)
                     /locus_tag="PK252_10645"
     CDS             complement(19885..>24791)
                     /locus_tag="PK252_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT15208.1"
                     /translation="TCDPLDSLYYTIYSNPFLTNEEKPVPLSCNLNVNGEMIADTNNL
                     DINGLPRTHIGAVEFVDNVPFSGGTYTIGSKGYFKTIKEAFSRLSSCGITGPIVLSLT
                     DNVYNEYSMYVQHIAGISATNTITLKPAPSVVSTVNFATDESNGTPFRFRDIRYLTLD
                     GASNGTDSHNLRFVANDIGQYASATLLEFFPDTGNIRNITITNCEFVSTRYAEANMAA
                     INFAADRGASENIVIKNNIIRRANIGVRFAYESFPSQNIKIQNNVFGDAIFANSISDK
                     AISINGADFVEISGNKIQNIINAYEALGIELYNIGTPEIFNNVISNIIGDGSETSSAT
                     GVYLTLSENTQNALIANNVINHLGGYAGATGLFVEIDATTLPSGGVKILNNTISLNKD
                     SFVALGSSGAQVNGVRLFSSSYLQKKVTFLNNIVSVKLGDRAFTTSGTSGNGLHISNK
                     GLFAESNYNLIEVSGNDVNYIVATDSAGGVNLASIDNWKAYSSGFDVNSVSAAPNFVN
                     DSSWMLAYNSPCINAGKPAFTGLSAVGITKDVRGWNRIVNGTIDIGAVEFEALQITSQ
                     SANQNICQGTNATLVVTTNYPEIASYRWQVSSNNVTWADSSAATNDTLVVNSQNYYRC
                     IVSAPGHANDTSNAILVNVNPLPMITLVSPDAICMGQNAIIKANTSAGIVSWYGSATG
                     GTVLAQNNIFTTPILTATTTYYAEASYNGCNSTSRVAATVTVNSFPAVEFKAVATNVT
                     NENNGTIKIATIKGTAPFSYSIDGGVSYSSTSLFSDLAANTYLVTVKDNNSCVTKQSV
                     IIGKCIADFIYTVTDSTLKLNNLSLGASQFYWTYGDGSVSNDSSPSYKYAAPGEYEVC
                     MTMVNMTTNCMANKCALIKIDSLNTSYLAAKYTYTINQLQVSFANISEKATNYYWIFG
                     DGQFSTEISPVHNYSRAGIYVVYLKAYNSSTGKSATYVKKIVVGTAVCSNEASFKYVV
                     DNAGKVTFTNTSIGKNAYYWTFDDGMSSTLANPVHTYKNAGYYLVSLMIKDSVGTCSD
                     AYSQLVKIGNIACKADFDYSVETSTNTVTLKNLSTGNIANNFWLFDGRNYSTMQNPTF
                     KYVSAGIHNISLTVLDKNGSCIDYIEKSVQVGKASCNANFAYYVDSVNNKVEFTSLDK
                     TGGTDAYWIFGDGTISTETNPVHIFKRAGYYQVSLTTFNPETGCMDNVSKIILVSGPE
                     NDCEADFIYLMGTDNQTVAFKNKSKGNIVKWLWNFGDNSAISNETSPTHLYKVQDDYN
                     VCLTVVNTQNIVNITCKEIKVGSSVGNENKADFIYQVSKDSAYTRFYNLSSGTYNKLE
                     WNFGDGKTSALASPAHKYSTPGYYMVSLKVTNTATNSHNVTYKLLNINKPDTFLVRFA
                     FWAKPYSKKAGGYPIDFIGAGLGDQAKLKWDFGDGETDSTTTSPQHTYQNSDEYEVCL
                     TYADPITGDSSKHCEKVTTATLCQSDEVSPEPLCKNIEVHLDANGKAVITPDLVDNGS
                     TDACGIKSKSLSVSTFTTTGTKDVVLTVTDNSGNYSTCQAKVTVLGYVGVETTENQLG
                     LNVYPNPFTAKLIIKMDIPDNGVVNLSIVDISGRNVRLTKQMTLEKGERFITLDGDML
                     EQGTYYLRVTDSKGRMKQTVIVKQ"
BASE COUNT         7751 a         5008 c         4337 g         7695 t
ORIGIN      
        1 gcagcattat ctggttactt ttatgagtgt ttacacaatc ttttttatac taccacttaa
       61 tcctcctgct cgaagatatg tatgatgctg ctctaaaaat aaagcgctat acaaaagata
      121 tggattttag ctcgtttgca aatgatgata aaactataga tgcagtaatc cgcaattttg
      181 aaatcattgg agaagctgcg aaccgtattg cccctgatta taaattcaaa aatccggagc
      241 ttgagtggaa ccatttaaaa ggcttcagaa acaggattgt tcatgaatat ttcggagtgg
      301 attatgaaat tgtctggtca attatagaga ataaccttga caattatata gaatggatag
      361 aagtcctttt aaataaagag aaaagtaatt agagcctatt ccaccacctt acctttcaat
      421 gtagcctgtg tacaaccagt aatttcgacc atcacatagt cgcccgggtt aaagttcatt
      481 ttaggaaata ctactacact attttgcgaa gtacggccgt atagctcatt tttcgatttc
      541 tttgaaacag cttcaacaag aacctcaaag gttttgccaa tatctttctt cttattttct
      601 gccgaaaggg tattttgtaa atctataatc tcggtgagtc gccttgattt aacatcttcg
      661 ggaacatcat cagtaaggtt atcggcagca taagtgcccg gacgttgcga atatttaaac
      721 ataaaggcag aatcataacc cacctcacgc atcagcgaaa gggtttgctg atggtcttgt
      781 tctgtttcgc cacaaaaacc agctatcaga tcggtagata tggtgcattc tggcatataa
      841 tgcttgatag ctgctatacg ttgtaaatac cattcgcggg tataacctcg cttcatggct
      901 tgaagaatgt tattgcttcc actttgcgct ggcagatgga tgttgcggca tatatttttg
      961 tacctggcca tagtacgtag taagtcatcg ctcaagtcct tgggatgaga agttgcaaaa
     1021 cgtacacgca atttcgggtc aaccaatgcc actaattcta taagttttgg gaaagttata
     1081 tttgctcctg ttgatgattt ccagagatac gagttgacat tttgtccaag cagcgtcact
     1141 tctctatacc cggcatcaaa aaggttacgg gcttcgttga caatggtttc ggggtcgcgg
     1201 ctgcgctctc ctcccctggt gtaaggcact acacaatagg cgcagaaatt ttcgcaccca
     1261 cgcatgatag aaataaaggc agtaacgtgg ttttcgtcta acctgaccgg gttaatatcg
     1321 gcatacgttt cctcgtgcga aagaataaca ttggcagctc tcatgccagt tcctgttttg
     1381 gctaccagga caggcaagtc gcggtaagca tcaggcccgg caataaggtc aactgccttt
     1441 tccttctcca gcaattgttc tttcatcctt tcggccatac aaccaataac cccaaccttc
     1501 aatgcaggtt ttttgcgttt tatggcccgt aactcacgca gacgtcccaa gacacgttgt
     1561 tcggcattgt cacgtatcga acaggtattg acaagtataa ggtcagcctc tgtaatgtct
     1621 ttggttactg caaaaccttc tttttgcata atggctacta ccacctcgct gtcattaaag
     1681 ttcatttggc agccatacgt ttcaatatat attttattgc ctgacgcttt tgtatattgg
     1741 tgcgatacct gcaattgccc cggttcaatc atgattgtaa aattttgagg tgcaaagtta
     1801 ttcgaattgg acagatttct tgtccaaaat tttttccatt aacaaaaaga gactgctttg
     1861 gttcaaacag ctttatacca aaaacatatt gataaatagc tgcaagtccc gtatgggact
     1921 atatattggt agaaaaagta tgtgaataat tgtgttaacg tgcctttagg tacgtaataa
     1981 taacaatttt aataaacaac tggtttgaag ataaaccgct gatcgtattc tattccgaaa
     2041 gctttcatat aatcctcgta ttcttcaata aatgttttat tcttatgatg aatttcttgt
     2101 gtttgtatgt aatttattac ttgttctgtt tgcgacttcc cataagaaaa agccccataa
     2161 ccttcttgcc atgaaaattt tccccgaata aactttcttt cattaatcca ttttgatgaa
     2221 tcacttttta actgctgcat taattcagat atagattccg agggcctcat accaaagaaa
     2281 atgtgtaaat gatccggcat gccatttatt gttaggagtt tatgatgatg gttttgaata
     2341 attccagtaa tgtatttata caactcatct ttccattgct ttccaataat acattcacga
     2401 ttttgcagac taaaaacaac ttgaatatga atttgagtgt atgtatttgc cataataatg
     2461 atttatattt cgtcccatac gggactaata acaataaaat aacaatatta ccaacaatat
     2521 cctgttccta acggaacaaa atcaaatata agataaattc ttttcacatt caaaaaagca
     2581 actaccccaa tcaaacataa atttttgttg cacagatttt tacaataaat aaaaatagaa
     2641 aagtatcatc ctgaacaaaa acaataacct tcagcaaaaa aatgatggat ggccacaaat
     2701 ggaagctttt ctgcctgatg tgccgtttta ttgggtgggg cttgctttgt ttattaaccg
     2761 caggtattgg tttcttgtgg ttaatacctt atatgtatgt tagttttgcc agattttatg
     2821 acgatgtgag agcaagttac gtagcatgtt tatcaataaa aaaggcgcct taggcgcctt
     2881 tttatttaca tatcttttta ccatccgggg ttttgtttta aaacgtcgga agataaactg
     2941 atttctgttt ccgggatagg gaggaaccct ccggtttcag gcctgaaaga agcgtcatac
     3001 atttcatcta cgccgctgtt tttaacaggg atgttggtaa cttttgcaaa agcagcctca
     3061 gcatcatgcc aacgaagaag gtcaaaataa cgaacccctt caaaagcaag ctcatggcga
     3121 cgttcttctt taatattttc taaagatgca ggttttgagg gcaatcctac acgtgccctc
     3181 acgcggtcaa aataagcctg ggcattgggg cctcctaatt cagctcccat caacaatata
     3241 tctgaaaagc gcaagataat ttcgtcttgt aaattccaca actggttgtt ggtatttgaa
     3301 cctggaacga gtatggtaaa taccccaaca taagaagctc cttgtttaac ctggagtgat
     3361 gtatattttt tattccacag gccagtttca tgcatacatt ggtttgaccc ccaaacatat
     3421 ttagttgaca cattaccttc ttcggggtcg gtaacatcaa taatagagcc ctttttgcga
     3481 atatcaccat cttcaaagct ttggttcagt tgaggattaa caggcccggc tccccaaccc
     3541 tggctccaag gtactaatcc ttcctgaatc ctaagcccca tatacaaaca aacctgattg
     3601 ctgtatgtca ctttagtcgg aaggttccat cctccatgag tcgaatattt aatagcaaac
     3661 acagtttctt tattttttag ctgtccatta gagtcttttt cgccagcatc tccaacccaa
     3721 cttaggttat tgttggttgc ataaggatat gagttaggca tagccatgtt tacacatgaa
     3781 taaggccaaa agttcctaaa atcaggggtc aaatcatgtc cactattggc aatacagtca
     3841 tctaaccaac ttattacgtt agcttttgta acagcgccgc cttcagtcaa aggcatctca
     3901 gttttgttgt aataaccggt gtagaataaa aaaatacggg ccatcatccc ttcagcagcc
     3961 catttggtag catgcccggc acgttcagaa gcaatgttat taacatttac ggttttcata
     4021 agctctattg cttttttcat atctgaagca atttgagcat ataatacttc aggttttgcc
     4081 tgaggtacaa cggcaaaagc agggtcggta attaaaggga tattgccaaa aaacttagct
     4141 aaagtaaaat gaaaataagc cctgagaaaa taagcttcgc caagatcctg atttttatca
     4201 gcttcattgt catactttgc ctggccaata cgatcaataa ttttatttac acgcaagatg
     4261 cctttataat attcttgcca aagtggataa taattgtcag cctggctatt ggtaaacttg
     4321 tcaaggtcat ggaaagtaat atcaccactt ccgccaccac caaagcaatc gtctgaaagt
     4381 aagttggcca tgactgtagg gtttgaggcc cctccataag aaggtaatac agaataagcg
     4441 ccagttaatg catcggcaac atcctgtggg gttttataaa agtctgcatc ggttttcagg
     4501 tacaattgtt tttgatccag aaaatcttca ccacagcctg ctaaaagcga tattgcagct
     4561 aaataaagta tatatttaat ttttttcata tttctaaaat tttatcatca attaaaacag
     4621 attaaaattt aaggctcaac ccaaccatat aagtttgtga agaaggatat aagcccaaat
     4681 caacacccga agcccatgaa tcaggagcat aaccaacctc tgggtccata ccaatatagt
     4741 tggtaaaagt gtaaaggttt ttgacagcaa cataaagctt agcttcagtt aaaggtaaac
     4801 ttttgaaaag atatttaaag tcatacccaa aggtaatgtt actaatcctg ataaaatctg
     4861 catcctcaat ataaatatct gatattttgt cagaagctgt atttaataac cttggataac
     4921 ggtttgatgt cccggctcca tgccaacgtt tcaaaatatc agtagtatag ttttgtttag
     4981 ggccatcaac aaacgacctg tacgacatgg ctacctggtg cccatatgct ccattggccg
     5041 atgccataaa ataaaaacct ttatacgaag catctaactg caaaccaagg atataatcag
     5101 gattggggtc tccaatcatt gttttgtcct tctcatcaat gatggtatct ccattaaggt
     5161 caacatatct ttggtctccg ggtttaatct tgtttaccaa ttgtcctgtt ttcttatcta
     5221 cagtataata agttgatttg cctcttgtaa acgcttctac ctcggcagaa tcctggaata
     5281 cgccatcggt cttgtatcca taaaaataac cgataggata atcaatttgt gcccggtaac
     5341 attcaacggt accttgactt aatacatttt ttgaaccatg aatgatactg tctgagctgt
     5401 tcaattgtgt tacacggttc ttgttgtaag ctatagaagc tgtaacccca tatttaaaat
     5461 ctccatcgcg gtcattccaa cgaagggtaa actcaacccc tttattagaa attgtcccac
     5521 cattagaata agctgcattg gtaccgccca tagctacgat tggggcctga actaaccaat
     5581 ctttggtttc tttattatag tagtcaaatg taaattgcag cttattgtta tataaataca
     5641 aatctaagcc taagtcagtc tgttccgatg tttcccaagt gaggtcgtaa ttggcaacat
     5701 gttcagggta acaaccggca gtacgggctg ttttatctgt tccaaagaaa taatctgaac
     5761 tattgtcata agtttgagca gccaggtatt ggaatttacc attaggcata tcctggttcc
     5821 cgttttgtcc ccaacttgct cgaagtttca aactacttac taagtcttta gaccaatcca
     5881 taaaaggttc atttgaaact aaccaaccgg cagatactga agggaaagta ccccaacggt
     5941 gtccttcagg gaagttagaa gaaccgtcag cacgtaatac aaaagtaaac atgtaggttt
     6001 ctttatagtc ataagataaa cgtccaaaat atgacaaaag gccccatcca tactcatctt
     6061 tgccagtaat ggtagtataa cttgcatcta caacaggtac gtttttaaga taagcatgtt
     6121 ctgcatcctg gaacaggctg ttttcatttt tcccctctaa agaaagattt tgtgtgtttt
     6181 ttgtcatttc attccctaac aggaatgtaa aattatgttg tccaagagcc tggttgtatg
     6241 ttgccgtatt tgtccatgtc caggtaaaac cttcatacat gctctggttt acaacatcac
     6301 gggtggtaaa tgtttttgaa gtcaaataat atttaggtac ccacgaacgt gatgagccat
     6361 accagtttga accggaaaaa gcagacctga atatcaggcc ttttataggc tgtaactcag
     6421 caaaagcact tgcgttgata ttattattgt cattataact tgaattgcaa ttgtattcca
     6481 taagagcaat agggttggcc tcttcgaggt tccattttgt tggaggggca taattcccta
     6541 ctgaatcata agctctcaaa atggggctgg taacaagcat attgtgcaaa tcgttccagt
     6601 aaatattgcc tgtgcggaaa gttggttttt tggtattggt aaatgtaagg ttttcaccga
     6661 cagtcaatat ggcattgtta tttttttccc aaataacctg agttgtgttc atacgcaggt
     6721 tgatacgttt gtatttattg ttggtttgtt ttcccaatac tccttcttcg gtaaaatatg
     6781 atgcccctaa tgaataggtc gactttttag agcctcccat aatattcaaa gcatgacttt
     6841 gagtaaccgc atttttgttt tcaatttcat ctacccaatt ggtaccattc cattctccac
     6901 tcatgatttt gtcataatca ggcactaatg ttgcccattt atatgctttt ttggcgtaac
     6961 cagcaggatc ggtgttgtaa ttggcttcat ccataatctc catatactgc tttgcattaa
     7021 gcatctcagg ttttttgtat atgtttgata ctccatagta tccatcatag gtcaccacgg
     7081 ttttcatatc ttttgcccca gttttggtgg taaccaaaac aacaccgttg gccgcacgtg
     7141 aaccataaat agctgatgaa gcagcatctt ttagtacatc aatagactcg atatctgaag
     7201 ggctcaaata atcaatatcg ccaacagcta taccatcaac aatatataat ggtttagaat
     7261 tcccaatagt accaacacca cgaatattaa cccttgtacc tgccccggga agaccgctat
     7321 tttgaatgat agtaacacca ggggttatcc ctttcaaagc ctctatagga ctgttggttt
     7381 taagggcctg gatattttct cctgcgacat tcagtgttgc cccggtattg agttttttct
     7441 tctgaactcc gtacccgata accactactt cattgagttc ttgtacatcc tctttcattg
     7501 ttacagaaaa gcttgtttgt ccgcctacgg gtatttcctg gctcaaataa cccacatact
     7561 taaattcaag tgttgcagag gcatccgctt taatagtaaa tttgccgtcc atatcagaaa
     7621 cggttccggt agttgtacct ttaatgatca cattaactcc agggagcgga gcaccactgt
     7681 tgtcaacaat agttccactg atagaacgtt cttgtccaaa aattactgcg cccgaaaaaa
     7741 gcaacagtaa tgaaagcagc actgacttta accgccagcc agccttttgc ttgtgcgaag
     7801 ttttgtacat agttgattga tttaattagt aattgttagt ttgttaaaat taacattttt
     7861 ttacaatggc aaatctaaaa aaaactacct gtccaaggtt gccttgtcct ttgtttttta
     7921 aagaagttaa caatatgtac agtatttttt taccaaatat ttccattttt ataatgatat
     7981 aaatactgat accaagataa ttactttaaa aattatcact gttgtcccgt aaaataaaag
     8041 atacttataa atactacgta atatcgttga atagaaactg ggcgccccgc tggggcttaa
     8101 ttttaaagat gtttaatttt actacaaaca ggacgtcgct acgcgactaa ataatagcag
     8161 cgtagctgcg aactgtttgt aggataacca aatacaccaa atgtttctat gccacatagc
     8221 cgagacctgt ttttaccgga caacaatgaa aaattatata gatagaacaa gttatttcaa
     8281 taattactaa ctttaaacca aaaacatgaa atacttactt tctcttgtat ttgtatcatt
     8341 gtttgttaca agcaaaactg acaaaacaac tactaccatg acatctgaag atttggccaa
     8401 aaatatccat tggatacagc aggctggttt taaaattgag gctggttcgc aaaagatata
     8461 cattgaccct gtaggcatca acgccaatga caatgccagt atagtgttga taacccatgc
     8521 ccatggtgac catttttcgc cagctgacat tgcaaaaata gcaaacgaaa acaccactat
     8581 ttacatgcct gcaagttgta actatgatgg taaatacaaa gctaaaacca tcgtaaaaat
     8641 tggtaacaaa tacattatca acgattcgct tactattgaa gtagttccgg catataatgt
     8701 ggtaaaaacc aagtttcatc aacataacga caattttgta ggatacatca taacctataa
     8761 cggaattaag atataccaca caggcgacac tgaacgtatc cccgaaatga aaaacattac
     8821 tgctaacatt attttagtcc cgcttggcca gacttatacc atgaacaatg ttgaagaagc
     8881 tgccaatgcc gtaaaagacg tgaaagctga aatagccatc cctatgcact ttgggctgta
     8941 cgaaggtaaa acatctgaca ccattgcttt taagcaatat cttgagggag cagctaaggt
     9001 tattatttta gaaaaaggga agtaaacttt aattatcggt ttagttgttg tttcaaccta
     9061 aaccttccag gttttggaaa cctgcaaggt tttctaacag tcttgactta ttgcccaacc
     9121 accttgaaat aaagaacaaa cctgttaggc ttttaaaacc tgacaggttt atttatattt
     9181 gccccaaaaa aatcatgaac ctaaaaatat ttgcattcgt agctggttta atccttttta
     9241 atcttttgca caccgaagcc cagcccaaaa aggcagtcct atcgggtaaa gaatttacac
     9301 cgcatagcat tttcattcat tatccctcaa cagaacatgc ctgggcaaac gcccaacgag
     9361 gcaatgaatg gatgctgaac cttaacggca gctggctata caacacatca ggttctactg
     9421 ccaggcttac tcctgaccga aaagggttca acccgcaaga atggcaatca tttcaagtgc
     9481 catcaaatat ttttggcgaa caaacagaac ttgacacaat gatacgccca aaaggcaata
     9541 aagttatcct ccgaaaattt ttccctatac ccgaaagctg gtatgggctt cagaccgaac
     9601 tggtttttga aggtattggg cccaaaggtg ttgtgctggt caatgggatg aaagcagcga
     9661 acatttctaa taatcaatat ggacttttta tagaaattac gccattactg cgttttggca
     9721 aaatgaacag catcgacatt atccttgaga atgtaaatgc cgcttctgcc gactcgttgg
     9781 ccgggttgtt tggaaatgtg gcgctttatg cccatcctaa tgttgctata agcgattata
     9841 ccatccaggc aacgcctgca tctcccggaa atgaaggtaa gattgatatt acccttttcc
     9901 caaaacgcta ccttgaagat attgacgata agtttaccat tgaaatgtac ctttatgaca
     9961 aaaaacgcaa taacctgctt gccccagtgg ccctgaaata taacccatca aaaaaagcag
    10021 actcagtgct gtttaaaagt atgacagtaa aaggggcaca ggcatggaca agctgccaac
    10081 ctgtatatta tacgctgcta atactgctaa aagataaaca aaaacaggtg gttgaagcca
    10141 taaaacaaat cgttgctttc agagacttta gatacgaagg agacagcatt attatcaatg
    10201 gcaaacgcga cacctgtatg acatttaaag agatggacag caagttggct attactggcg
    10261 aaccctttga aaaagacaaa ctttacaaac tggtatttac aagcaattta caacctacag
    10321 atataagcaa tattatccgc atagtacgaa aatacaggaa caacctgcaa cttgctgcct
    10381 acgaactaaa aggtttcaaa gatgatgcgg cacaaacaat taaagcagct attgaagcag
    10441 aagacaaaac acacccggta aagtattaag cgctatgcct cagggctacc aacactgccc
    10501 cggtaatata agctactatc caccaaataa aggctatacg cgaataaata tgcagctttt
    10561 tgccaacata agttaatgcg ccatattttt tacgaaaaat ccataacaga acagcatcaa
    10621 tgagcattaa agacaacgaa gaatagccta ttaaaccatg agcagtaaaa gcccccttag
    10681 aagaaccaat aaccatcagg gctgtagctg ttatatcaag aataaccccc aaagtgataa
    10741 aaatcaacac tttgttgctg acaagctttt tgcgttgttc agtaacaatg gcaatagcat
    10801 acgatatcag ggcaaggtta acaatagtag tacctgccat taaaacagtg ttcatctcgt
    10861 ttgtcgtttc tttaaaaata ataatagcat cgatattaag cccataaaaa taatacttac
    10921 agcttgtgct ccatgactca gcaaagcata agccaagcca tcgctccagg agatggcata
    10981 tatagaaagt gcaagggcta tcattccatg ataagctcca ataccagcct gtacaggagc
    11041 tgcaaaacca taactcccta caaccaacaa aaataaggca tcaaccattg tcaagtgggc
    11101 tgtaggctgc atggctttga ggcaaatgta ggtcatcagc aaaaaaaaca cccaaatagc
    11161 caaagttgaa gcaaagaaag cctgctttcg ttttaggtac aagattgact taaagccaat
    11221 gaaaacacct ttggctattt tttttatttt ccctactaaa gtgattttgc gtatctggtt
    11281 cctgaaagca tataccaaga aaaagccggc tgcaacagct aaccctgcaa gtatccaggt
    11341 ataaattgaa aaatcaaaaa gccacccaaa tttttttcct aacgggataa atactttttg
    11401 cgaaaaaaaa cctccaaatt ctttgatttt taacaatatc acaagtattg tcagcaaaaa
    11461 aaggacaagc acgtcaaata tcctttcggc aataacagtc cccagcaacg catctgcagg
    11521 ccttttttcg gtcctgccca gcactccgca acgggttact tcgccaattc gcgggaaagc
    11581 ataatttgcg gtatatccaa taattaatgc atggtaagtg tttgcaaaac gcggcttgta
    11641 accaagtggt tctatcatca actgccaacg gtacgccctg gcaagcatcc ctccaaaacc
    11701 aaagaacaac gataatacta ccaaatcgta acgggtatgc ttcaggttat ctacaagcac
    11761 ctgaaaatca atgctgcgaa atgcaaagta aagtaatatt atgccaaggc caaggaaaat
    11821 agcatattgc aaaaagatta ataaaaatct cttggttttg ctcattgtac aagtttgttg
    11881 gtgcgggtat cagggaatat aaccgtaggt ttaaagcctt tggctttctc tacctctatc
    11941 atgccataag acatcactat cacaacatct cctacaacaa ccttgcgggc tgccggacca
    12001 ttaagcgcta taacacccgt acctctttcc cctttgatgg tatatgtttc aaacctttca
    12061 ccgttattga tgttcacaat ttgtaccttt tcgccttcaa acaggttggc agcatccata
    12121 aggtcttcgt caatggtaat gctcccaaca taatacaaat tggcttcggt aacagttacc
    12181 tggtgtatct tcgattttaa tacttcaatc cacatttttt gaaagaatga tttgaatttg
    12241 gtgcaaagtt ataccatata ttttggcaaa agtagttttt caccttattt aaacttcaaa
    12301 acgttgattt ttgtttaata gcaggaacgg ctccttaact tttcccaaaa aatttgtatc
    12361 tttttatttc gtagtagtcg ataaccttac ggacaaggct tcttttaaaa aacctttggt
    12421 aagcttttat aatttgtccg tttaatggag gcgtggtcaa ctttatcgtg ttgttactat
    12481 catccagctt tgtttcgaac gaacggctct ttcgtacatg cgtacctgag ccatctgccc
    12541 caatattcct aacttttgaa actgtcggaa aaacgtttaa cttattaaac ttataactgg
    12601 tatagcaaaa acgtatagcc caagagttat tctggcctag ttgctgtttt aacaacatat
    12661 ttgaacaatc ggcccccccc atgttgaacg cctcccgttc ttttttgttt cttataaaag
    12721 tatcaaagtc ttcaactttc cagtcaacca gctgccacct atctttccag gtagcccagc
    12781 cccatgagtt tattcgataa aaaagaaaaa catcttcttt gtaatcagca ggaatggtaa
    12841 ttggggcaca ataacctgaa atagaaaata tctgtccgtt atgctcataa aaatcaagac
    12901 agtcattcat gtacctcaaa aagtttggac taaattccag gtcgtcttcc aatacaatta
    12961 tcctgttgtg agccccaaaa acctttgaaa ccccatcaat aatcgaattg gcaaggcctt
    13021 tatttacctc tgaaaatgta taatggatat ttttaaaacc tcttgcttca gcaattacgc
    13081 gttttacttc atcaacagcc aaaacatctt ttacccccct ggccccgtct gaaaaaatat
    13141 acaattcgct ttccctggcc aaatcattct ttgccaaaga atccagcgtt ttcttcaaat
    13201 gtaccggacg gttaaaacaa aacaaagcta ttggcgctaa cataaatcgc taattaaaat
    13261 tctctattct aaaagtcaaa agtgataaaa aaacctaata cacgttgaca aatgtataag
    13321 gtttttatca gaaaatgaat attgttttac aatcaaagca aaatcaaaca acctatgtaa
    13381 atatgaaaaa acatcctaca tttgtgttcc taaaatattt ggtatgccaa caaataatga
    13441 tatgctttca ggaagcaaca agcttagtat ttttcatccc cgaaaatata tccttactaa
    13501 agtaagaaaa gaattacaaa aggtttttga taaaaatatt acaaaaactg atggaatgct
    13561 tttggattat ggatgtggcg atatgcctta tcgaaattat ttgtcatcaa aagtagaaaa
    13621 atacataggg gcagacatag aaggtaatga taaagccgat atgctcatac ttcccaacgg
    13681 gagaacgcca ttggctgaca atacatcaga ttatgttatt tcaacctctg tccttgagca
    13741 cgttcccgat gtagctcttt atcttaacga gactgcaagg gttttgaaaa aagaagggct
    13801 aatcatactt actactcatg ggatatggat gtaccaccct catccaactg atttttggcg
    13861 ttggacatct atgggcttga aaaaagtgtt tgaagataat aactttgaaa ttgtgcattt
    13921 tcaaggtgtt ggttctcgtg ccgctatggg attgcttctt tttcaggatg ggttgtattt
    13981 taaacttccg gtattcatca gaaatatctt ttccatgttg atgcaacctt ttatttgtct
    14041 ctttgataaa gtttctaaaa aaaatactac ggataaagat gccggttttt atattgtagt
    14101 agctaaacct aaaaaattat gagtatctgt attgcatacc ccaataaaaa cctctctttt
    14161 agtgaaactt ttatttccaa tcatctcaaa tttattgaac acaaatctga actcactgga
    14221 ggatattatc cttacttaac cattgacagg aatagtatat ttcaatttcc tttaagcatt
    14281 gacctattaa gaggcggtat aaaaaacatt gctccaaaag tttataaaag gctctatact
    14341 ttaaccctgt cagattattt gctcaaaaca aaaccaagtg ttgttttggc cgaatatggt
    14401 gttacaggca gcatgatgac tgatgcatgt aaaaaaacaa gtattccttt agtaactcat
    14461 tttcatggat tcgatgctca ccaatacaaa atcctggaac aatataaaga tgattatcaa
    14521 aaaatgtttg attatgcccg taccatcatt gttgtatcag aagacatgaa aaaagaactg
    14581 atacaaattg gggctgacga acacaaaata gtcaacttgc cttatggtgt tgatataaat
    14641 aaattttcgg gggctaatcc agaaaatagt gcaccgattt ttatatttgt tggacgcctt
    14701 acccataaaa aatcaccaat taatcttatc aaagcattta aaattgttgc tgacgaaatt
    14761 gatgatgcgc ggttggtgat tgttggtgat ggtgaactta aaaaacaaac ccttaatgag
    14821 attgaaacac ttaacctttc cgcgaaagtt gaactcaaag gcgtattaag cccgctacaa
    14881 gtagccgaag aactgaaaaa agcccgttgt tttgtgcaac attctgtgcg agatatcaca
    14941 ggagatagtg aaggaacacc caatacaata cttgaagcaa gtggaagcgg attacctatt
    15001 attagtacta cccatgcagg aataaaagaa gctgtagtac atggtcatac tgggttgctt
    15061 tgtgccgaac atgattataa ggccatggga caaaatatgc ttactattgc caaagacaag
    15121 cagcttgccg cacaaatggg cataaaagcc cgggaacata tatccaaaaa ttataacatt
    15181 gaaacgcaga tggaaaagct gcgccaactt ttatattaac ctttaatacc agaaaacatg
    15241 attgttcgcc taatcaataa agttatcaaa ccattaaaat taaatattac ttccatatct
    15301 tcagcagcac ctgtcgatat tgaaaaagat ttccttgaag cttaccaatt ggcaaaaccc
    15361 tataccatga catcttttga caggatgtac gctgcataca aagctgctga atacgtggta
    15421 aacgccaaga ttgaaggcga tattgtcgaa tgtggtgttt ggcgcggagg tagttcaatg
    15481 attatggcat taaaacttac tgctatgaaa tcctttgacc gtgacttata tatgtatgac
    15541 acctatgaag gcatgagcga gccaacgaaa gaagatgttg acttaagtgg cgttcattct
    15601 tctgaaagac ttagtaaaga agataagtat aaaggaaaca atgtatggtg ctacgccgga
    15661 ttggatgagg taaaacaaaa catgctgaaa accaaatatc caatggataa gattaagttt
    15721 attaaaggaa aagtagaaga caccattccc ggcacaattc cttcaaaaat atctatcctt
    15781 cgattagata cagactggta tagctctaca aaacatgaga tgcagcacct tttccctctc
    15841 ctatccaaaa acggaatatt gattatcgac gattatggac attggcaagg ttccaggcaa
    15901 gcggtagatg aatattttaa agaattcggc attgtcccat ttttacatag agttgattac
    15961 actggccgtc tttatgtaaa actttagaaa cattccatca taacttaata aataatgaaa
    16021 ctttagtaag ctaaatgaaa agaattattt atatttgata ttaacattta catgaaaaca
    16081 caatccctta aactcgcctt ttatacgttt ttgttgttaa tatttagcat ttcttgccaa
    16141 aagaatgaca actctgagtc tcaacccgca accgggcaag ttcaatttgg ttttaaatca
    16201 ggccaaatga atgtaaaaag ctcgtctgtt aatgctactc aagtctgata gtttctgttg
    16261 aagataaaga tgtgtttttg tttgcatact tttgcatgca atctaataaa aaactatggg
    16321 catagttgtt cgtcaaagca ttaaagcaac agttgtttcc tatcttgggg cgctggtagg
    16381 ttatataaac ataatgttta ttactccatt gtgtttgtct ccagaaataa ttggttttgg
    16441 aaaggttttt ttagatgccg cgttgatgtt tacctttgta gcccagcttg gtttaaactc
    16501 cgctcttata cgctttttcc cgtattttag agatgaaaaa aacaaccatg gcatatttgg
    16561 gataatacta gttgtaccgc ttatgggttt agtactttta tctgcactgg tgggaatttt
    16621 ttacaaccct atcattggca tgttccaagc caattcggca ctctttgcgg acaatttcta
    16681 ttatgtgctc ccgcttacat tctttatgat gtatttggtt ctgtttgaaa ccttttcatc
    16741 atgcctgctt cggattgttg ttccaaaatt gatacgggaa attgggctta ggttattgaa
    16801 cattgcagtt attgtgctat tttatcttaa agtactggat tttaaagctt acattatttc
    16861 aatcatcgtt atatatgtta ttgcaacagt tctcaacctt tactacctga agaaaattac
    16921 aaacctatcg ataaagcctg atttctcagg tattcctaaa aaagtatttc gtgatttgat
    16981 gttttacata gctttcattc tttttgttgg tattggttca aatattgtca gcaaaataga
    17041 tgtattcatg attagttcga gcctaaacct cagttatgct ggcatcttta gcattgcttt
    17101 ttatgtaaca acaataatag agattccgtc aaaagctttt atgcaaatta ccaatcctat
    17161 tgtagcacag gccgccaaag acaatgactt caaaaaaata ggcgatttgt acactaaaag
    17221 ttcaataaac caatatattg ttggaggttt cattctgttg ctcgtatggg ccaatgttga
    17281 caatatattc aaaatcatgc ccaatggaga gcttttccaa tcaggtaaat atgtgatcct
    17341 gcttcttgga cttggcaaga ttgctgatat ggtaactggc ataaacctaa tagtaattgc
    17401 aaactctagg tattattggt atcagcttat atttacttca tatatcacag caacaacaat
    17461 acttttaaat tattacctga ttccaaggtt tggtattaat ggagctggca tgacttcttt
    17521 aatcgccatg agcacctata atttgcttct gctgtttttc cttaaatata agctctcgct
    17581 ttggcctttc aacataaaga cccttacatt aaccatcttt ttccttttac tttttgcaat
    17641 caattacatc ttacccgttt tatcaaatcc tattttagat ggtatttata gaagtattat
    17701 tcttacagca ggctttgtat tagttatcta caaaggcaat atttcagatg attttaacaa
    17761 cctgatatca caaatcagtc ttgaaaatat taaaaaacat ataccaacca aaatcaaata
    17821 gtgatgaagg tcttgttttt attaggaggg atgcctcatt atatttcaaa aataataaaa
    17881 ccaaacttgc cggcatgagc ggactatcta tcaacgaagc tatggccttt ggaaaacccg
    17941 tggtatgttc ggtttgtgat ggtactgaaa aagttttggt ccgtgatggc aaaaatgggt
    18001 attactttga aagtggcaat agcaaaagtc ttgcagataa gattatgtcc attattggac
    18061 atgatgaagt gcaaaccagc atgggcaaag aaagtgaacg tatcattaaa gaagaaatca
    18121 atattgacgt tgtttgtaac aactttattt cggccttacg aacaatccac tcatcgtgaa
    18181 gaatctcaaa acatccatat tattggtttt tagtttcctc tttcttgcta ctattaattc
    18241 agaaagtcag catctcaatt ttcaaaacga atacattata aagtttgtaa aacccaccaa
    18301 tgtaaaaatc gaatacctga ctaccctcat ttctattgac aaagtaaaag gtgatagtat
    18361 tgttgcctac gtaaatacta aacaactaaa gacatttcaa aagctaaaca tcccgtttag
    18421 ccttattatt aaagaaaaag caacattagc aactacctct ggcaatattt gggattggaa
    18481 caaataccct acactcagcg aatatataca aatgctggat agtatgcaac aaatccatcc
    18541 cgacctttgt aaaataataa catttggcaa tagtgcctta ggaaaaccta tatattacgc
    18601 tcgaatcaac aaagatacct ccatatacaa accatctgtc ttactttctt ctactataca
    18661 tggcgatgaa acaggcggct ctgtggtttt gaccaggcta attttttatt tgcttgacaa
    18721 ttatggcaaa tcaagtaccg taaccaacct cattgacaac ttggatatct ggatcaaccc
    18781 ggttttcaac cctgacggaa ggtttgccag cactatgaac cgtagtaatg gcaattatgt
    18841 tgacctcaac cgtaattttc ctgatatttt ttacggttct cacccagata ataatgatta
    18901 ccagcctgaa acccggtctt taatgaactt gtactcaaca tacaattttg tattatcggc
    18961 tactttacat gctggagccg aagtagccaa ttatccttgg gatgcatccc cttcatttca
    19021 tgccgataag caatggtttg aaaatgtatc acacgaatat gccgatactg ctatgaaata
    19081 tggccctgtc aattacttcc aaacgcttta ccctgatggt gttattgatg gctggtactg
    19141 gtatccggtt tatggtggcc gccaggatta tgtcacaggc gtttgccaca gccgcgaaac
    19201 aacgctggag atagatgaca acaagtttac tcccgaaaat gaactggaaa ccctttggca
    19261 agccaattat cgctcacttc tcaattatat caacaacgcc accaaaggca ttcatggaat
    19321 tgtctatgac aaaaacacca ataccccgat aaaagctact attgaggtgg taaactacga
    19381 tgattcattg tctgtcgtct attcggatac taaaacaggc cattttacaa ggcttttacc
    19441 agtaggaact tataccctca agatttatgc aaaaggttat gacacgttga ctgttgaaaa
    19501 tacatatttt acaatgaata catcaagaca acttacagca taccttactc cttctgctaa
    19561 aaattacatt gataataact tttcaggaca aattaaggtt ttcccaaatc ctgctatgaa
    19621 taccttgttt atttcaggca tttatgacaa tgaaatagta ctaaaattat gtatttataa
    19681 tacttctgga gtttctatcc cagatctttc tttagaaaaa aacactcaca ccattgacat
    19741 ttctcacata cctgatggct tctattttgt atttattcaa acttcaaaaa aggtttttaa
    19801 atctaagttc ataaaaagaa caaattgaaa ggtactcaat tcttttgaaa caaaaagggc
    19861 aacctcacga ttaccctttc aatattattg ttttacaatg accgtttgtt tcatccggcc
    19921 tttactgtct gttacacgca gatagtatgt gccttgttca agcatatcgc catcaagggt
    19981 gataaacctc tcgccttttt caagtgtcat ctgcttcgtc aaccgaacat tgcgtcccga
    20041 aatatctaca attgacaggt tgacaactcc attatccgga atgtccatct tgataattag
    20101 cttagctgtg aatggatttg gataaacatt aagccccagc tgattttcag tagtttcaac
    20161 tcctacgtaa cccaatacag tgaccttagc ctgacatgtt gaataattgc cactattgtc
    20221 ggtaactgta agcaccacat cttttgtacc agtagtggta aaagtagata cacttaatga
    20281 cttagactta ataccacagg catctgtact gccattatct accaaatctg gtgttatcac
    20341 agcttttccg ttagcatcaa gatgaacttc tatgtttttg catagcggct ccggagaaac
    20401 ttcatccgac tggcacaggg ttgctgttgt caccttttcg caatgtttac tcgaatcgcc
    20461 tgtaattggg tctgcataag tcagacaaac ctcatattca tcgctgtttt gataggtatg
    20521 ctgaggactt gttgttgtag agtctgtttc tccatcacca aaatcccatt tcaacttggc
    20581 ctgatcgccc aatcctgccc caataaagtc aatgggataa cctccagcct tcttactgta
    20641 tggttttgcc caaaatgcaa atcgtacaag aaatgtatca ggcttattga tattcaacag
    20701 tttatatgtg acattatgac tattagtcgc agtatttgtc acttttagtg ataccatata
    20761 ataccccgga gtactatatt tatgcgctgg agatgcaagg gcagatgttt ttccatcgcc
    20821 aaagttccat tctaatttat tatatgtccc tgaagacaaa ttataaaacc tggtataagc
    20881 gctgtctttt gaaacctggt aaataaagtc tgctttattt tcattgccaa cagagcttcc
    20941 tacctttatt tccttgcaag tgatattaac tatattttga gtatttacta ccgttaaaca
    21001 tacgttataa tcatcctgta ctttatacag gtgtgtaggt gaagtttcat ttgatatagc
    21061 cgaattgtca ccaaagttcc ataaccattt tacaatattg cccttcgatt tattcttaaa
    21121 tgccaccgtt tgattatctg ttcccattaa ataaataaag tctgcctcac agtcattttc
    21181 agggcctgat actaaaataa ttttcgaaac attatccata catccagttt cgggattaaa
    21241 ggttgttaag cttacctgat agtaacctgc ccgtttgaag atatgaacag ggtttgtttc
    21301 ggtactaatt gtaccatccc caaaaatcca ataagcatca gtccctccgg ttttatccag
    21361 actggtaaat tcaactttat tgtttacact gtcaacataa taggcaaagt tagcattgca
    21421 gctggctttg ccaacctgga cactcttctc gatataatct atacagctgc catttttatc
    21481 caaaacagta agagaaatgt tatgtatgcc ggctgaaaca tacttgaacg ttgggttttg
    21541 cattgtcgag taattacgcc cgtcgaagag ccagaaatta ttggctatat ttccggtcga
    21601 aaggtttttc agagtaaccg tatttgtaga agtttctacc gaataatcaa aatctgcctt
    21661 gcatgcaatg tttccaattt ttaccaattg agaataagca tcactacaag ttccaactga
    21721 atctttgatc attaaactta ccaaataata cccggcattt ttataggtat gcacaggatt
    21781 ggccaatgtt gatgacatac catcgtcaaa cgtccaataa taagcattct taccaattga
    21841 agtgtttgta aatgtcactt tcccggcatt atcgacaaca tatttgaaag aggcctcatt
    21901 actgcataca gcagtaccta ccactatttt ttttacatag gttgcagatt tacctgtgct
    21961 actgttataa gcctttagat atacaacata aatgccggca cggctataat tatgaaccgg
    22021 agatatttct gttgaaaact gtccatcgcc aaatatccag taataattgg ttgctttctc
    22081 cgaaatattg gcaaaactaa cttgcaactg gtttatagta taagtatatt ttgcagccag
    22141 atagcttgta ttcagcgagt ctatttttat cagcgcacac ttgttggcca tacagttggt
    22201 tgtcatattg accatcgtca tacacacttc gtactctccc ggggctgcat atttgtaact
    22261 tggactactg tcattgctaa ctgacccatc tccataagtc cagtaaaact gagatgcccc
    22321 taacgaaagg ttattcaatt tcaaagttga gtcggttacc gtatagataa aatcagctat
    22381 acatttacca ataattaccg attgtttggt aacacagctg ttgttgtctt taactgttac
    22441 aaggtatgta ttcgcagcta aatctgaaaa caagcttgta cttgaataag ataccccacc
    22501 gtctatcgaa taagagaatg gggctgtccc cttaattgtt gcaatcttta ttgtaccatt
    22561 gttttcgttt gtaacatttg ttgcaacagc tttaaactct actgccggaa aactgttcac
    22621 tgtaacagtc gctgctactc tgcttgttga gttacaacca ttatagctgg cctcggcata
    22681 atacgtagtt gtagctgtca ggattggtgt tgtaaaaata ttgttttgcg ctaatacagt
    22741 gccccctgtt gcactaccgt accatgaaac tatgccggca ctggtatttg ccttaataat
    22801 agcattttga cccatacata tagcatctgg actaactaat gttatcatgg gcaaagggtt
    22861 tacatttacc aatattgcat tggaggtgtc atttgcatgc cccggggctg atactataca
    22921 tcgataataa ttctgactgt ttacaaccag ggtatcattt gttgcagccg aagaatctgc
    22981 ccacgtaaca ttattgctac tcacctgcca cctgtatgaa gctatttcag gatagtttgt
    23041 agttactacc aaagttgcat ttgtcccctg acaaatattt tgatttgcac tttgtgaagt
    23101 aatttgcagt gcttcaaact caactgcccc tatatctata gtgccgttta ctattctgtt
    23161 ccaacctcta acatctttag ttatcccgac agcacttagc ccggtgaagg ctggctttcc
    23221 ggcattgata catggtgagt tgtatgccag catccatgat gaatcgttga caaaatttgg
    23281 tgctgctgaa acagaattta catcaaaacc agaggaataa gctttccaat tatctattga
    23341 agctaaatta acaccaccgg ctgaatctgt tgccacaata taatttacat cattaccact
    23401 tacctcaatg aggttataat tgctttcggc aaacaagcct ttatttgaaa tgtgcaatcc
    23461 attacccgaa gtacctgagg tagtaaaagc cctgtcccct aatttaacag aaacaatatt
    23521 gttcagaaag gtcactttct tctgcaagta tgaagaacta aataatctta ccccattgac
    23581 ctgagcacca gaagagccaa gcgcaacaaa tgaatctttg ttaagagata tggtgttatt
    23641 caatatcttt acccctcctg atggaagagt tgtagcatca atttcaacaa acaaccctgt
    23701 agctcctgca tatcctccaa gatgatttat tacgttattg gctatcaaag cattttgagt
    23761 attttcgcta agggtaagat aaacacctgt tgctgagcta gtttcactcc catcgccaat
    23821 tatattactt ataacattat taaatatttc aggagtgcca atattgtaca actctatacc
    23881 taatgcttca tatgcattaa taatgttttg aattttgttt ccggagattt ctacaaaatc
    23941 ggccccatta atgcttatag ccttatcact aatactattg gcgaagatag catccccaaa
    24001 gacattgttc tgaattttta tattttgtga cggaaacgat tcgtatgcaa agcgtacacc
    24061 aatatttgcc ctgcggataa tattattttt aataacaata ttctcagaag cacctctgtc
    24121 tgcggcgaaa ttaatagctg ccatgtttgc ttcagcatac cttgtactta caaactcgca
    24181 attggtaata gtgatattac gtatattacc tgtatcgggg aaaaattcaa gcaatgtagc
    24241 acttgcatat tgtcctatat cattggctac aaatctcagg ttatggctat cagtcccatt
    24301 gctggcacca tctaaagtaa gatatctgat gtctctgaat ctgaaaggag tcccattgct
    24361 ttcatctgta gcaaagttta ctgtagaaac taccgatggt gcaggcttca aagtaatagt
    24421 attggtggcg cttatgcctg caatatgctg cacatacatg ctatattcat tataaacatt
    24481 gtctgtcaac gacaaaacaa ttggcccggt aataccgcat gacgacaagc gggagaaagc
    24541 ttctttgatg gttttaaaat aaccttttga accaatggta taagtacctc cactaaaagg
    24601 tacattatca acaaactcaa cagcccctat atgagttctt ggcaacccat ttatatcaag
    24661 attgttggta tctgctatca tctcgccgtt tacattgagg ttgcaactta gcggcacagg
    24721 cttttcttca ttggtcaaaa atggattgga ataaatagtg tagtacaagc tatctaatgg
    24781 atcacatgtt g
//
LOCUS       DAOSPF010000054        24338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2076_length_24338_cov_7.585718, whole genome shotgun sequence.
ACCESSION   DAOSPF010000054 DAOSPF010000000
VERSION     DAOSPF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_24338_cov_7.585718"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..3300
                     /locus_tag="PK252_10650"
     CDS             22..3300
                     /locus_tag="PK252_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15209.1"
                     /translation="MAISIIWIITFFTTNQHITYSQAVSSTFDKVTIKDGLSQSTVNC
                     IIQDKHGFMWFATYGGLNKYDGYSFTVYQNDENDSTSIGSNINEVLFEDSEGFIWIVN
                     NGNGGLDKFDPESGIFYHYKHDPKDPASISSSTVNYVMQDKSGNIWVCTNNALNLAVH
                     EKGNRGAKTTFKRFMSPSGNNFVRAYEDSKGNLLLFSKNLYYFDRQTNTFTASIQLPN
                     ERIISFCEDKKQNIFVSTQTAGVFKLEWDNNSSRYKLGDNAKINPAPKNRCCVTIDNF
                     GMLWIGTETKGLFRFNPETEQMVNFVPDKLDPRSLSDGNIYSLRVDRSGVLWIGTYSQ
                     GLCKYDLYRKDFALFKSIPGNINSMSGNVISGITSSNANELWVGSRDGGGINRFVFNA
                     NEEPKVFHYLNNRNDINNIVDISCLSLLQRKNGDVWIGSQGFMTKMVPEKIGSNKRPQ
                     VTRYPMQGWTFNLFEDSQGILWGGTWGSGLWRYDDASREFVYFRNDANNPFSLCDNVV
                     WSIGEDSYGNLWIGGHSEGLSILPAGERGKANPKFVNFRYKKGDLKGLSNNTIGAICR
                     DHSGTMWLATDNGLNKCIDKGNVLKNLNENSKLEFKSYYKKDGLPGNGIVGIVEDKAG
                     NIWLSTNNGISKFNVADTTFTNYSEDDGLQSNEFWHNASYINSEGRIFFGGSNGFNAF
                     YPDKIKANEFLPAVVITNIEIVNKPIKPGEEINGEVVISKPIYMTPSISLSHKNNMIT
                     LAFAGLHYAKPSSIKYAYYLENFDKEWIYTDKRTVTYTNLDPGTYTFRVKATNNDGRW
                     NEEGTSLIIEVRPPWWATIWFRILAIASIIGGIACFFSYRTKKLKENQRILELKVSEA
                     TDKVNAQNLKLKEAQEKLTNIMSDVKNQLGKASEELLDASNSQASTSEEISASMEEIA
                     SEMAENASSMLQMLETVKQVEVEAEESVTIVKNTLTSINEISASIKYVSEIARITNLL
                     SLNAAIEAARAGENGKSFAVVAAQVKKLADQSAEVALKIENASANGQRLSKEANDKII
                     QLNNIINGIVGTISEINQSIQTQSIEANNVNSSILQMSMYISNTSDLAEKLDAAINSL
                     TIEE"
     gene            3440..5275
                     /locus_tag="PK252_10655"
     CDS             3440..5275
                     /locus_tag="PK252_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOT15210.1"
                     /translation="MKNRLLFLLRYFLFWYLFFFLGKVLFLLFNLRQTSELDLVSILK
                     IFLYGAKLDISACSYLLVLPSLLVTTSVFIAKDVFKPIVKIYTIVFLVIFSFLIVADL
                     ELYKYWGFRIDTTPLLYINTPKEMLASVSIWVVVRQMAIFAVILYVFIKFYNKFVNEK
                     VDFTVKSKIFYALIYLLLAASLIIPIRGGFGLAPINTGAVYFSSNEFANHSAINPIWN
                     AGFSVLQTEAMSNPYKYYKEEQLQDEIKGLYTHSTDTTKVLKEQRPNIILIVLESFMG
                     KAIEPMGGLKGVTPRLNKLCNEGVFFTRMYSSGVRSDKGLVSVISGFPAQTKASIIRY
                     PNKTQAMPSIAKELNSNGYHSAYYYGGDIDFASMRSYITNAGFEKIVDMTDFPKSTYG
                     AKWGVHDHYVFKRLLHDIDTSKGRFFYTFFTLSSHEPFDVPMNPVIEGNDENTQFLNS
                     LYYTDSCLGDFIDSARTKAWWNNTLVILIADHGTLRLGVQNSYDFDRFHIPMLWIGGA
                     VDKKIVVEKIASQTDLAATLLAQLNILPQKSFNFSNNILDNKNTGFAYSAYNSGFCFI
                     QGNDSVAFDADENKVKYQSKNASEKVVNTGKAMMQFTYGAFLKMK"
     gene            5277..5777
                     /locus_tag="PK252_10660"
     CDS             5277..5777
                     /locus_tag="PK252_10660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT15211.1"
                     /translation="MVTFRTVTTNDLELVQDLAYTIWQSHYPGIISHEQIDYMLRKMY
                     NTQQIEKELKSGVRWELVEVNGKAIGFISFHHEKEENFVKLSKLYILLEYHGKGYGQQ
                     TLQYVEGEAKKTGASKLYLTVNKNNEKAIKAYLKAGFIKERSEVFDIGCDYVMDDYIM
                     AKPLNQ"
     gene            complement(5771..6436)
                     /locus_tag="PK252_10665"
     CDS             complement(5771..6436)
                     /locus_tag="PK252_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT15212.1"
                     /translation="MMIAENIKHIINSIPSDVQLVAVSKTKPNADIIEAYHVGQRLFG
                     ENKVQELVAKYESLPKDIQWHFIGHLQSNKVKYIAPFVSLIHSVDSLKLLQIINKEAE
                     KCGRTIPCLLQVHIAKEETKFGLDQTELNQLIEESGNLKNVCICGFMGMATNTDDMNI
                     VRNEFRELKRLFDTAKSKYFNQSSSFNTLSMGMSGDYKIAIEEGSTMIRLGSTIFGER
                     IYH"
     gene            complement(6656..7630)
                     /locus_tag="PK252_10670"
     CDS             complement(6656..7630)
                     /locus_tag="PK252_10670"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT15213.1"
                     /translation="MIPYGNDIEKAAQIIKAGGLVAFPTETVYGLGASAFNKVAVARI
                     FEVKQRPSFDPLIVHISSIDQLNMLFESPIEPLVYKLAELFWPGPLTIVHKKKAGVPD
                     IITSGLDTVAVRMPSHTTARKLIELAEIPIAAPSANRFGRISPTKPQHVQKQLTGVEY
                     LLTSDEAVKIGIESTVVAIDGDTCNILRPGAITLEHLTNNGIKAESNKEKASKALSSP
                     GLLKSHYAPLKPLYMFSRLPETFPSNSGIIVHSNKYNLPDNIKTAITSDNNTMTEIAA
                     NLFESLHQMEDDPSIDNIFIEVVEKNGIGIAIMDRVQKAAFHTEQRYL"
     gene            complement(7642..8112)
                     /locus_tag="PK252_10675"
     CDS             complement(7642..8112)
                     /locus_tag="PK252_10675"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT15214.1"
                     /translation="MGKLVIIMGSKADMDWSQKIADAAVKLGMEVAMRIASAHKVPLR
                     CYEILKEYEKENPVFVTVAGRSNALSGFADAQTHCPVIACPPYGDKFAGNDIYSSFRM
                     PSGVAPMLITEPDNAALAAAKIFGITNANIRKKIIEFQESQRKKLIDDDTQLLK"
     gene            complement(8210..8446)
                     /locus_tag="PK252_10680"
     CDS             complement(8210..8446)
                     /locus_tag="PK252_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15215.1"
                     /translation="MHLKTVAIHSRNTFWSSRDQLDELGVDAEDLAREIETKCNELEK
                     DGYKVIKIVPINSGNQVAGSGAHYTESVLVVAQK"
     gene            8719..10662
                     /locus_tag="PK252_10685"
     CDS             8719..10662
                     /locus_tag="PK252_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT15216.1"
                     /translation="MFSLQKNVEHVDQSLLPVKTEIEKLLVYNRQFRDKILSQSANNG
                     FSENIIADSIEKKVIPLIDIVKKQQKSQYSNLDFQLDADFSNFKADYESYKSYISKFA
                     AVEDSVKLLHKSFMDKKAKVDEVCNSVIAGNNSANDIQMKYRVNTCLAIINYSKDLNT
                     IYEAFANGQNLNHFQVLFALKLLEGLKSFYNNSLSSGYASKTVVIKQLQLNSDSLNAV
                     LVRYAQLMTDVTQSKEILLNKNLAFEHSLTETNEKLTNLTNRLLSEAGGQVQLSILMA
                     ILLAGIALLVLIVVFIWVSNGYARSVERGAAYALALSQGNTAENVEEHLSKYQFQLYS
                     ALTTIAEKMDIFKKNEIVSADKQKELSKIHEIVTNEIWQNLKKQKEQLEVLKDNVEKY
                     ELLNDNKLKNIDQWSKQQDNNIDSVKHCNQMAADTMSTMALIEEKVKVITDIAFQTNI
                     LALNASIEAARAGEHGRGFSVVAAEVRRLAEISNAAAQDINNLYMASLEKTKNIVGSL
                     NDSLARHEQNNTIIAKNINVKSEQNTNAGEIKQRIDAILNLCINLMHKVNNISHEDAI
                     ELANKPLFKTQITENNTKYTPEYPDLKMKKQVTEDITIEMQRYNDAETKKSKINGKEV
                     KINGQKSKFIIRQQRQPKQEQIF"
     gene            10684..11541
                     /locus_tag="PK252_10690"
     CDS             10684..11541
                     /locus_tag="PK252_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HOT15217.1"
                     /translation="MQIERLNEIFRAKLSDDDFKRLSDFIYSHYGIKMPPVKKIMLQS
                     RLQKRLRELNITTFKDYVEFVFSPEGQDNEVIHMIDVVSTNKTDFFREPVHFEYLTDV
                     VIPAYAASNPRMPLTIWSAGCSSGEEPYTIAITIEEAKQKYPNIDYTILGTDISTRIL
                     KSAHEAIYKENRVEGIPISILKKYFLRSKDRSNPTVRVIPELRRKVSFGRLNFMDNYY
                     QVQENFDVIFCRNVLIYFDRETQEKVISKLCSKLKPGGHFFLGHSESITGMHLPLKQI
                     KPTIFSKIE"
     gene            11589..12485
                     /locus_tag="PK252_10695"
     CDS             11589..12485
                     /locus_tag="PK252_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT15218.1"
                     /translation="MSKLTDQELIDELQRRFEENKKTIQELKELTEELKIVNKKLEES
                     EALKSHFISNITNEIINPFTSILGLSRSILSVKKENWKKVISMVALIHSEAFNLDFQL
                     RNIFLAAKIEAGEIYPEILNVDLRTLVKNVIDTFRIESKKKNIVIELNFDIKPANGLV
                     FYFKTDPEKIKLALANLVSNAIKFSYESSKIIVRVWKEGDTVFMSVQDFGTGISEANQ
                     KIIFDRFKRLDSGINSLNRGHGLGLSINKAVIDLLQGKITIKSEVNKGATFTISVPEA
                     KTNIAGFSADGNEFLFDNEEIF"
     gene            12497..12976
                     /locus_tag="PK252_10700"
     CDS             12497..12976
                     /locus_tag="PK252_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOT15219.1"
                     /translation="MEENVHFLYPASLFASKSPYLVTTILGSCVAVCLYDANLQIGGI
                     NHYMLPLWNGQGLASPKYGNIAIEKLIEKMVSLGCDRNNLKAKVFGGGEVIETTVTQF
                     NIGARNIKLAHEMLEELKIPIVGQSVGGKLGRKILFNTETFEVKQKFIQKSNPLDHL"
     gene            12999..14081
                     /locus_tag="PK252_10705"
     CDS             12999..14081
                     /locus_tag="PK252_10705"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOT15220.1"
                     /translation="MRRIKVLIVDDSAVVRQTLTTILESDSQIEVMGAASDPFFAAKK
                     IQSEVPDVITLDIEMPRMDGLTFLKKIMSQHPIPVVVISSLTSEGTETGMKALEYGAV
                     EIITKPQMNTKQFIEESRIRICDAVKAAALSKIKRKTYSEITVAPKLSADAVIPIVKA
                     PTAMIKTTETVVAVGASTGGTEALQIFLQGMPIDCPGIVIVQHMPEFFTRSFANRLNE
                     ICKITVKEAENNDSVIRGRALIAPGNHHMLLKRSGARYYVEVKDGPLVNRHRPSVDVL
                     FRSTARYAGNNAIGVIMTGMGDDGARGLLEMKEAGASTVAQDEKSCVVFGMPNEAIKL
                     GAADKIIPLDQIAAQVVKMVALRENS"
     gene            14166..16436
                     /locus_tag="PK252_10710"
     CDS             14166..16436
                     /locus_tag="PK252_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15221.1"
                     /translation="MLKRSYKYTIWGLVSGIFFITILWFAGSIINNIPFSLTSFAYLH
                     NIYPIFWYIDLLPLIMGLLAFIIEKENFNFEILLEENLKHETQKSQNVLDFVNNLIQD
                     NFDFEIEDLNNGDKLSGALVELRNKLKQNKAEEIIRRKEDDQRNWISEGLAKFSELLR
                     RNNNDLKQMSYEIIIELVKYIGANQGGVFLLEESKSGERYFMQTAAYAYDRKKFANKR
                     IEWGEGLIGTCALERKSIYLTEVPDSYLNITSGLGKANPRSVLIVPLQFNNEIHGIIE
                     LASFEKFEKFEIEFVERIAESTASTLASVKINLRTADLLKESREQAEILAAQEEQMRQ
                     NMEELQATQEEAARQSEKFISFTNSVNHTLIRAEYLVDGTLIYANTKFLNKLGYASNL
                     EVEGKHISMFINKKDCEWFEPLWEELANGGKHFEGYMKHVTKLGQDLWTMATYTCVRK
                     EDGNVEKILFLAIDTTEQKKQSLDYEGQIDALNRSSIKVELQPEGRIMSINALLTESL
                     GYTDHEMVGKKMIDFIHKEDLDNFKAIWKNVLGGQPFMGQIRKVGKNNEVKWFRGTYT
                     AVMDMYGDVSKVIFIANDITKKKQMEIEASRQNEQLKEQEEKLRLAGVELSLKLKEAK
                     EEMRMQFKEIERIKVRNELTLEGALDAIITINEVGTIEFFNKAAEMLWGIDRKDAIGQ
                     NVSILFDNETIENDEFVASYVKPGTDKIVGVRKEVQIKTREGEQKPVLFLISMAKVDN
                     SYAYTAFIQNIEVELF"
     gene            16454..18088
                     /locus_tag="PK252_10715"
     CDS             16454..18088
                     /locus_tag="PK252_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15222.1"
                     /translation="MNTSSEKEQGKSKQIDTSALKFSFLQDNSPYSIFDYEVFLHSLK
                     LSKSAITIFRPSGQEFVITKSIINLLQVKSDIAIIREYFLSRGFDKYDGQLIMAAINK
                     VIESGEATEEMLYLKDSSLHKRNKVYRILIQPAALWGFNNSADLILHNTDISEVSHLY
                     DDLAKEQEKVSEGIVVKNAFLNNISHEIREPMNTILGFTELLTLGNIKDEQRTDFLNI
                     VKNKSRAMLSMIDDISELARFESDGITVARMETNVPKLLNELLLEYHKELDIRRKSNI
                     ELILKLPSDKDSYVIYVDSGRLYQVLNYLLDNALKYTDKGFVQFGYEVKDAKQLQFFV
                     RDTGGGIPKDEQKYLFTRYRKKEEVLDKPILKKGLGLTISRYIVELMGGKIWVDSTVG
                     EGTTFYFTVPFGKEINSIVSEANFVEKPFLNWRNKVILIVEDEVVNYQFLEAVFAESQ
                     AQLVHVTDGDKAVELCKTLNNIDLILMDIRLPVKSGYDAVKEIRQFRPAIPIIAQTAY
                     SSKKERDKCKAVGFNEYITKPIDIPLLIQMINKYFN"
     gene            complement(18081..18635)
                     /locus_tag="PK252_10720"
     CDS             complement(18081..18635)
                     /locus_tag="PK252_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT15223.1"
                     /translation="MKKLFLFLSSILAPTSFVVNAQSIKPFYDFKPVAIDGSTFDFAQ
                     LKGKKVMIVNTASKCGFTKQYEGLEALYKKYSGKNFIIIGFPSNNFASQEPGTNEEIA
                     NFCKINFGVTFPMMAKINVKGDSIHPLYQWLTQKELNGQFSSVVKWNFQKYLIDENGK
                     LVKVYYSITKPDDKKIVEWIESTN"
     gene            complement(18693..21350)
                     /gene="leuS"
                     /locus_tag="PK252_10725"
     CDS             complement(18693..21350)
                     /gene="leuS"
                     /locus_tag="PK252_10725"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT15224.1"
                     /translation="MEYNFKELEAKWQAYWEKNKTFKTSNDTSNPKKYYILDMFPYPS
                     GAGLHVGHPEGYTATDILARYKRMKGFNVLHPMGWDAFGLPAEQYAIQTGTHPAIVTN
                     RNCDNFRRQIKMLGLSYDWDREINTTDPKYFKWTQWIFIRLFNTWFDEQQQKGRPISE
                     LPIPDDIKCQGNKAVKEYIESKRLAYYAEAQVWYCPHCQIVCANEEVLTDGSHEKCGN
                     PVLRKNLKQWMLRIPLYAERLLQGLDTVDWPEGIKEMQRNWIGRSTGAEVNFEIDGKP
                     EKIKVFTTRPDTLFGATYMVVAPEHPMVDTITTADKKAEVKDYIEKARLKSDLDRTEL
                     AKEKTGVFSGTYAINPVNGNKIPIWIADYVLMGYGTGAIMAVPAHDTRDFEFATKFNI
                     PIICILDPVTADADTRAQVLAGKACWTEDGEYINSANANNGLDINKLNKENGLKTIIK
                     WLEKTGKGTEKTNYRMRDWLFSRQRYWGEPFPVIHWEDGEITVLDDTQLPLSLPNIEK
                     YQPGENGESPLANAGEWLQVTDSKGRKGRRETNTMPQWAGSCWYYLRYIDPENDTAPF
                     DKKLEKYWMPVDLYVGGAEHAVLHLLYSRFWHKVLFDLGIVSTSEPFQKLFNQGMILA
                     YAYETMTGAKVPSDLVEEREGKYFHKDTGEELRQIIAKMSKSLKNVVNPDDVVQTYGA
                     DALRLYEMFLGPLDATKPWAENGVKGVFGFLTRASRFFGDKNNYFQGTEDIEVLKLLH
                     RSIEKISGDIENLRFNTAISQMMIFTNLCIKKQKVTTSTGEAFAKMLAPFAPHLAEEI
                     WEILGHSTTLAYEPFPEAEKQYLEDDFAVYAVSFNGKRRFELSLPKNMDVATVEAEVM
                     KHEQSARWLEGKAPKKVIVVPGKIVNIVV"
     gene            21509..22855
                     /locus_tag="PK252_10730"
     CDS             21509..22855
                     /locus_tag="PK252_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT15225.1"
                     /translation="MKRIREYIKKYYLSYISIGFDCSDNPVYIRKAKNALLVFSLSIL
                     LIVVLIVKELQEGKTWVMPVHALRIVALLGCIYLLYRKYYYASVRLGIYAIFISSIIG
                     TCIAPVQNSFHFLSFSICALSFYFNNRRVENVVFFILCLFYFSVSQYYNNYHLPPDNS
                     ASYIISLVIIFFIFYLTTALFVSEYKFFQGLIMSKNQELESKNSDLELQNRELEKLNR
                     FKNDSISIISHDLKSPVNGLVEMCYLLKKNFKNYTPEKFEQRIELLKYNSEKVNNMLI
                     ELLVWANSHSHAIEVQKKLVEVTDLCNNILVNNQFGASLKNVALSLQQYDSKYEVVSD
                     PVIISIVVRNLIANAIKYSKSGGSIEIDIKKDDNAVLISVIDDGIGISENVIKLIEER
                     AVLSSLPGTKAEIGHGIGLSICKDLCRLIDADFRLKRKSKEGGTIATLIIPFGSFL"
     gene            22957..23922
                     /locus_tag="PK252_10735"
     CDS             22957..23922
                     /locus_tag="PK252_10735"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HOT15226.1"
                     /translation="MQISKLIILSSVLLFSARAKAQDSIRIVDFPTQLHAFFKINSSN
                     NEIEISNPNISDELNYRPNLGTNIQLGFSYSWLGLAYSIMLPADNSMNKKYGKTSGFV
                     LEGHFMKPRIMIDVAYKRFKGFYLANPSSFITGWNNSLAYPHSADLGTSMLSFNCFYV
                     FRPQRYSPTAAYAFTKAMRKRGGSWMLGCFGAKSFIFSDSSIVSQSIHQYVNPELDLK
                     EMAFYEFGLSFGYSYLLTILKKNFVSFTLMPGVSVQSISQKSAIDNSINNRNAISQKT
                     VFRVSMGRNGNNNYYGIVANGETSVVKNSSTELSFISNYFLWTPL"
BASE COUNT         7752 a         4103 c         4956 g         7527 t
ORIGIN      
        1 tctataaaaa aaaattctcg attggcaatt agtataattt ggataattac tttttttact
       61 actaatcagc atattacgta ttctcaagct gtaagttcaa cttttgataa ggttactatt
      121 aaagatgggc tgtcacaatc gacagtaaac tgtattattc aggataaaca tgggtttatg
      181 tggtttgcaa cctatggcgg ccttaataaa tatgatgggt actcttttac ggtttaccaa
      241 aacgatgaaa acgacagtac aagtatagga agcaacatta acgaagtctt atttgaagat
      301 agcgagggct ttatttggat agtgaataat ggaaacggag gattggataa atttgatccc
      361 gaatcaggga ttttctacca ctacaaacac gaccccaaag accctgccag tataagcagc
      421 agcacagtaa actatgttat gcaggataaa tccggcaata tctgggtatg tacaaataac
      481 gcacttaacc ttgccgtaca cgaaaaaggt aacagagggg ctaaaactac tttcaagcgg
      541 tttatgagcc cttcaggtaa caattttgtc agagcttatg aagattcgaa aggcaatttg
      601 ttgttgtttt ctaaaaacct gtattatttt gaccggcaaa ctaatacttt tacagcctct
      661 attcagcttc ctaatgaaag gattatttca ttctgcgagg ataaaaagca aaatatcttt
      721 gttagtacac aaaccgccgg agtttttaaa ctcgaatggg acaacaacag ttcaagatac
      781 aaacttggcg ataacgccaa aataaacccg gctccaaaaa acagatgttg tgttactata
      841 gataattttg gaatgctttg gattgggact gaaacaaaag gactgttcag gttcaatcct
      901 gaaacagaac aaatggttaa ttttgtcccg gataaattag acccccgctc tctcagtgat
      961 ggaaatatat attcacttcg tgttgaccgt tccggggttt tatggattgg cacctacagt
     1021 caggggcttt gcaaatatga cttatacaga aaagatttcg cgctcttcaa atcaattccg
     1081 ggtaacatca attccatgag tggcaatgtt atttcaggca ttaccagtag caatgcaaac
     1141 gaattatggg tcggtagccg tgacggagga ggtataaaca ggtttgtttt taatgcaaac
     1201 gaagaaccta aggtattcca ttacctgaac aataggaatg acattaataa tattgtagat
     1261 attagttgcc ttagtttgct gcaacgaaag aacggcgatg tttggattgg ttcacaagga
     1321 tttatgacca aaatggtgcc ggaaaaaatt ggttcaaaca aacgtcctca ggttacaaga
     1381 tatccaatgc agggatggac ttttaatttg ttcgaagata gccagggtat tttatggggc
     1441 ggcacatggg gtagtggttt atggcgctat gatgatgctt ctcgcgaatt tgtatatttc
     1501 agaaacgatg ccaataaccc tttcagcttg tgcgacaatg ttgtatggtc tattggcgaa
     1561 gatagttatg ggaacctatg gattggaggg cattccgagg ggttaagtat tttgcctgcc
     1621 ggagagcggg gcaaagccaa tcccaaattt gtaaatttcc gatataagaa aggcgacctt
     1681 aaaggcctta gcaacaatac tattggtgca atttgccgcg atcattccgg aaccatgtgg
     1741 ttagctacag ataacggttt aaataaatgt attgataaag gcaatgtact gaaaaacctg
     1801 aatgaaaaca gcaaacttga gtttaagagc tattataaaa aagatgggtt gcctggcaat
     1861 ggaatagtgg ggatagttga agataaagca ggtaatatat ggttgagtac aaacaatgga
     1921 attagtaagt ttaatgttgc cgatactaca tttaccaatt attcggaaga cgatggcttg
     1981 caaagcaatg aattttggca taacgcatca tatataaact ccgaaggaag aatatttttt
     2041 ggaggctcaa atggttttaa tgctttttac cccgataaaa tcaaagcaaa tgaatttttg
     2101 ccggcagttg ttataaccaa tattgaaata gtaaataagc cgataaaacc cggagaggaa
     2161 attaatggtg aagtggttat ttcaaaacca atatacatga cgccttccat ttctttatcg
     2221 cacaaaaaca atatgattac tttagcgttt gccggattgc actatgcaaa accttcgagc
     2281 atcaaatacg cttattatct tgaaaacttt gacaaggaat ggatttatac cgataaaaga
     2341 acggtaacat ataccaatct tgaccctggc acatatacct ttagggtgaa agcaacaaat
     2401 aatgacggac gctggaatga agaaggcact tcgttaatta ttgaggttcg tcctccctgg
     2461 tgggccacaa tatggttcag gattctggcc attgcatcta ttattggtgg aatagcatgt
     2521 tttttttctt atagaacaaa aaaattgaaa gaaaaccaac gaatacttga attaaaagta
     2581 agtgaagcta ctgataaggt taatgcccag aatttaaagc ttaaagaagc tcaggaaaag
     2641 ctgacaaaca ttatgagcga tgttaaaaac caattaggca aagcttcaga agaactcctt
     2701 gacgcaagca atagccaggc ttcaacatct gaagaaattt cagcttcgat ggaagaaatt
     2761 gccagtgaaa tggctgaaaa tgccagtagt atgcttcaaa tgcttgaaac agtcaaacaa
     2821 gtagaagttg aagctgaaga aagtgtaacc attgtaaaga atacgttaac ttctataaat
     2881 gagatttctg cgagcattaa atatgtttcg gagatagccc ggataaccaa tttgctttca
     2941 ctcaacgcag ccatagaagc tgccagggcc ggagaaaatg gaaagagctt tgctgtagtt
     3001 gccgcccagg ttaagaaatt ggctgatcaa tctgctgaag ttgcattaaa aatcgaaaac
     3061 gcatcggcta atggtcaaag gttatcaaaa gaagccaatg ataaaatcat tcaactgaac
     3121 aatattatta atggcatagt tggcactatt tccgaaatca atcaatcgat ccaaacccaa
     3181 tctattgaag caaataatgt caattcatca attttacaaa tgtcaatgta tatctctaat
     3241 acatctgacc ttgcagagaa acttgatgcc gctataaact ctttaactat cgaagaataa
     3301 taaaaatcaa tttatttgta taagttttgc taaaaattaa gctatggggt ttcaaacttt
     3361 ttggtagttt tgctattgct atagcagata atgggcgcat agcaacatta ctttttattt
     3421 taatgaacag attatctcga tgaaaaaccg gctgcttttc ttattgcgtt attttctttt
     3481 ttggtatttg tttttctttt taggcaaagt gctattcttg cttttcaact tgaggcagac
     3541 ttcagaatta gacttagttt ctatactcaa gatcttcctt tatggggcta aactggatat
     3601 ttcggcttgc agttatttgt tggtgttacc gtctttgttg gttacaacaa gtgtgtttat
     3661 tgcaaaagat gtatttaagc caatcgtcaa aatatacact attgtatttc ttgtaatatt
     3721 tagctttctg atagttgccg atttggaact atacaaatac tggggattca ggatagatac
     3781 tacaccgctg ttgtatatca atacgcctaa agaaatgctt gcttctgtaa gtatttgggt
     3841 agtagtgaga caaatggcca tttttgctgt tatattatac gtatttatta agttttataa
     3901 caagtttgtc aatgaaaagg ttgattttac tgtaaaaagc aaaatctttt atgctctgat
     3961 atatttattg cttgccgctt cacttatcat ccccataagg ggcggatttg gccttgctcc
     4021 catcaatacg ggtgctgttt acttctccag caatgagttt gctaaccata gcgctataaa
     4081 cccaatatgg aatgcagggt ttagcgtttt gcaaaccgag gccatgtcta atccttacaa
     4141 atactacaag gaagagcaac ttcaagatga aataaaaggg ttatataccc attcaacgga
     4201 tactactaag gtattaaagg agcaacgccc aaacataatt ttgattgtgc tggaaagctt
     4261 tatgggaaag gctattgagc ctatgggtgg attgaaaggt gttacgccca ggttgaacaa
     4321 gctttgcaac gagggcgtgt tttttacccg catgtatagc agtggtgtcc ggtctgacaa
     4381 agggcttgtt tcggttatta gtggctttcc tgcacaaaca aaggcttcga taatcaggta
     4441 ccctaacaaa acgcaggcta tgccttcaat agcaaaggaa ctgaacagca acggatatca
     4501 ttcggcatat tattatggcg gagacattga ttttgcctcc atgcgtagtt atatcaccaa
     4561 tgcgggcttt gagaaaattg ttgacatgac ggatttccca aaatcaacct atggggcaaa
     4621 atggggtgtt catgatcatt atgtatttaa aaggcttttg catgacatag atacctcaaa
     4681 agggcgtttt ttctatactt tctttaccct tagtagtcat gaaccttttg atgtgcctat
     4741 gaatcctgtc attgaaggaa atgatgaaaa tacccagttt ttaaattcgc tatattacac
     4801 cgatagctgc ctgggcgatt ttatcgatag cgcaaggact aaagcctggt ggaacaacac
     4861 gttggttatt cttattgccg accatggtac attgaggctg ggagtgcaga attcatacga
     4921 ctttgaccgc tttcatattc caatgctctg gattggaggg gctgtggaca aaaaaatagt
     4981 ggttgaaaaa attgcctctc aaactgattt ggctgcaact ttgctggccc aactcaacat
     5041 tttaccgcaa aaaagtttta actttagtaa taatatcctg gacaataaaa atacaggatt
     5101 tgcttatagt gcttacaatt cagggttttg ctttatacaa gggaatgata gtgtggcttt
     5161 tgatgccgat gaaaataaag taaaatatca aagcaaaaat gcatctgaga aggttgtaaa
     5221 tactggcaaa gcaatgatgc agtttactta tggtgctttt ctaaaaatga agtgatatgg
     5281 ttacatttcg gacagttaca acgaacgacc tcgaattggt gcaggatttg gcctatacca
     5341 tttggcaaag ccattatccg ggcatcatca gccatgagca aatagactat atgttgcgaa
     5401 aaatgtacaa tacgcagcaa attgaaaaag agctgaaaag cggtgtccgg tgggaactgg
     5461 tagaagttaa tggtaaagcc attggcttta tttcgtttca ccatgaaaaa gaagaaaact
     5521 ttgtcaagct cagtaaatta tatattttgc tcgaatatca tggaaaaggc tatggacagc
     5581 agactttgca atatgttgag ggtgaagcta agaaaactgg agcttctaaa ttatacctta
     5641 cggtaaacaa aaataacgag aaggccataa aagcctatct gaaagctggt tttatcaaag
     5701 aacgaagcga agtatttgat attggctgcg attatgtaat ggatgattat ataatggcta
     5761 agcctttgaa tcaatgataa atgcgttcgc caaaaatagt gcttcccagg cgtatcatgg
     5821 tggaaccttc ttcaatggct attttgtaat cgccagacat gcccattgat aaggtattga
     5881 acgaactgct ttggttaaaa tacttgcttt tggcggtgtc aaataggcgt tttaattctc
     5941 tgaactcgtt gcggacgatg ttcatatcgt ctgtgttggt ggccatcccc ataaacccgc
     6001 aaatgcaaac gtttttcaaa ttaccagact cttctattaa ttgatttaac tcggtttgat
     6061 cgaggccaaa tttggtttct tctttggcaa tatgcacttg tagcaaacag ggtattgtcc
     6121 tgccacattt ctcggcctct ttgttgataa tttgcaacag cttcaggctg tccaccgaat
     6181 gaatgagaga tacaaaaggg gcaatgtact ttactttgtt gctttgcaaa tgccctataa
     6241 aatgccattg tatatctttg ggcagcgact catattttgc aaccagctcc tgcaccttgt
     6301 tttcgccaaa caagcgttgg ccaacatggt aggcttcaat gatatcggcg tttggtttgg
     6361 tttttgaaac agctacaagc tgtacgtcag aaggaatgga gtttataata tgttttatat
     6421 tttcggcaat cataatagct atgttttacc ttgcaaaggt agtatgaaaa gaagatttat
     6481 cgcaaaacgc tttgcatttt ccgataggtg caaagcattt taaatagcaa aaaacttgat
     6541 agttacgggt tactttttta tctttagtaa ctttaatttg ggttaaaaga atattttgta
     6601 aacacaaact cccgttagct accggaatat caaaatcagt tttatgcgta tcaaattata
     6661 aatacctttg ttctgtatga aatgcagctt tttgtaccct gtccataatg gcaattccaa
     6721 taccattctt ttcaacaact tcaataaata tattatctat agaagggtcg tcttccattt
     6781 ggtgcaatga ctcgaataag ttggcagcaa tttctgtcat tgtgttgtta tctgaagtga
     6841 tagctgtttt tatattatca ggaaggttgt atttattgct atgtactatt ataccagaat
     6901 ttgaagggaa tgtttccggc aacctggaaa acatatacaa aggtttcaaa ggggcatagt
     6961 ggcttttaag taaacctggc gatgaaaggg ctttagatgc tttttctttg ttggattcgg
     7021 ctttaattcc attattagtc aggtgttcca gtgtaatagc ccctgggcgt aatatgttgc
     7081 aggtatcgcc atctatagca actactgtgg actcaatacc aatctttaca gcttcatcac
     7141 tggtaagtag atactcaact ccggtaagtt gtttttgtac atgttgaggt ttggttgggc
     7201 ttattcgtcc aaatcggttg gcacttggcg ctgctattgg tatctcggca agttcaatta
     7261 attttcgcgc agttgtgtgt gatggcatgc gtacagcaac agtatcaagc cccgaagtga
     7321 tgatgtcggg tacccctgct ttctttttat gcacaatagt caatggcccc ggccaaaata
     7381 gctcggcaag tttataaacc aaaggctcga tgggtgattc gaacagcata ttgagctggt
     7441 ctatagatga aatatgtaca attaacgggt caaacgaagg cctttgtttg acttcaaaga
     7501 tacgggctac agccactttg ttaaatgccg aagctcccaa tccatatact gtttcggtag
     7561 gaaaggctac taaaccacct gctttgataa tttgagcagc cttttcaata tcatttccgt
     7621 aaggaatcat caatgtttta tttacttgag gagttgtgtg tcgtcgtcga taagcttctt
     7681 acgttgactt tcctggaatt cgataatttt tttgcggata ttggcatttg taataccaaa
     7741 tattttggct gctgccagtg ctgcgttgtc tggttcagtg attaacattg gcgcaacacc
     7801 cgatggcatc ctgaaacttg agtaaatatc attgcctgca aatttatcac cgtaaggagg
     7861 acaagctatt actgggcaat gagtttgagc atcagcaaat ccactcaaag cattgcttcg
     7921 tcctgcaaca gtaacaaata ccggattctc tttttcatat tctttcagta tttcatagca
     7981 cctcaggggt actttgtggg ccgaagcaat acgcatggct acttccatcc caagctttac
     8041 tgctgcatca gctatttttt gagaccaatc catatcagcc ttagaaccca taatgataac
     8101 taactttccc ataatttttt tgtttgcaaa tttcaaaaaa ataagcaata tctacacaag
     8161 acaacaacat tctgtattgt cccggcacaa aattttcaaa aatgtaggtt tatttttgtg
     8221 ctacaacaag cacactttcg gtataatgcg ccccagagcc tgccacttga ttgcccgaat
     8281 tgatgggtac tattttgatt actttgtatc catctttttc aagctcatta cattttgttt
     8341 ctatttccct ggccaaatct tcagcatcaa cgccaagttc gtccaactgg tcgcggcttg
     8401 accagaaggt atttctgcta tgtattgcaa ctgtttttaa atgcatataa acttcttata
     8461 attcttcaaa tttaaattat tattagaata aattatgtat tagatgaaac tttgttgaga
     8521 gtttattttt atctaaaaaa atatgttcgg tcaaaaaatt tcgatagaaa ggccttgttt
     8581 cgaaaaaaaa ctctaaattt attgtcgctc aatttaatac gtaaaacaat gaacctccac
     8641 tttcgcaaaa aattaaattt tttccttgtt atatctggat ttttattgtt cccaatagtt
     8701 gtgttagcag tattttatat gttttcatta caaaaaaatg ttgaacatgt tgaccagtct
     8761 ttattgcctg ttaagacaga gatcgaaaaa ctgcttgttt acaaccggca attcagggat
     8821 aaaattctta gtcaatctgc aaataatggt ttctcagaaa acattattgc agattctatt
     8881 gaaaaaaaag taataccttt aattgatatt gttaaaaagc aacaaaagtc tcaatattca
     8941 aatttagatt ttcagttaga tgctgatttt tctaatttta aagctgatta tgaatcatat
     9001 aaaagctaca tctcaaaatt tgctgctgtt gaggattctg taaagctttt gcataaatca
     9061 tttatggata aaaaggcaaa agttgatgaa gtgtgcaaca gcgttattgc aggaaataat
     9121 tctgccaatg atattcaaat gaaatatcgc gtaaatactt gtcttgcgat aatcaattat
     9181 tcgaaagacc taaatactat atacgaagct tttgccaatg ggcaaaacct caatcatttt
     9241 caggttcttt ttgccttgaa attgttagaa ggtcttaaaa gtttttataa taatagtctt
     9301 tcaagtggat atgcttccaa aactgttgtt ataaaacagt tacagctaaa tagtgattcg
     9361 ctaaatgctg ttcttgtgag gtatgcacag ttaatgacag atgtaacaca aagcaaagaa
     9421 atactgctga acaaaaacct tgcttttgag cattcattaa ccgaaacgaa tgaaaaactc
     9481 acaaatttaa ccaacagatt actttcagaa gcaggaggcc aggtgcagct tagcattttg
     9541 atggctattt tattggcagg cattgctttg ttagtgttaa ttgttgtttt tatttgggtt
     9601 tcaaatggtt atgcccgttc tgtagaacgt ggagcagctt atgcacttgc attgtcgcag
     9661 gggaatactg ctgaaaatgt agaagagcat ctttctaaat atcagttcca actttattcg
     9721 gctttaacaa caatagcaga aaagatggat attttcaaaa agaatgaaat tgttagtgct
     9781 gacaagcaaa aagagcttag taaaatccat gaaattgtta caaacgaaat ttggcaaaac
     9841 ctcaaaaagc agaaggaaca gctggaagtg ttgaaagata atgttgaaaa atatgagctt
     9901 ttgaatgata ataaattaaa aaatattgac cagtggtcaa aacaacaaga caataatata
     9961 gattctgtaa aacattgcaa tcaaatggct gcggatacca tgagtaccat ggccttgata
    10021 gaagaaaaag taaaagttat tacagatatt gcttttcaga caaacattct tgccctcaat
    10081 gcatctatag aagcagcaag ggcaggtgag catggacgag gtttttctgt tgttgcagcc
    10141 gaagtccgtc gcttagctga aataagtaat gctgccgctc aggacattaa caacctgtat
    10201 atggccagtc tcgaaaaaac caaaaacata gtgggctcac tcaatgatag tttggccagg
    10261 catgagcaaa ataacactat tattgctaaa aatataaatg taaaatctga acaaaataca
    10321 aatgctggcg aaataaagca acgtattgat gcgatattga atttatgcat aaatttaatg
    10381 cataaggtaa acaatatttc tcatgaagat gcaatagaat tggcaaataa acctttattc
    10441 aaaacacaaa ttactgaaaa taacacaaaa tacacacctg aatatcctga tttgaaaatg
    10501 aaaaagcagg ttacggaaga tataactatt gaaatgcaga gatataatga tgctgaaaca
    10561 aaaaaatcaa aaataaacgg aaaagaagta aaaattaatg gacaaaaatc taaatttatc
    10621 atacgacaac aacgtcaacc aaaacaagaa caaatatttt aaagttattg aaaatcaatc
    10681 tgtatgcaaa ttgaaaggtt aaatgaaata tttagggcta agctttcaga tgatgatttt
    10741 aagcgtctca gcgattttat atatagccat tatggaataa aaatgccgcc agtaaagaaa
    10801 ataatgcttc aaagccggct gcaaaaacgc cttagagaat taaatattac cacatttaaa
    10861 gattacgtag agtttgtttt tagtcctgaa ggccaggaca atgaagtgat tcacatgata
    10921 gatgttgtga gtaccaataa aacagatttt tttagagaac cggttcattt tgaatatctt
    10981 acagatgttg taattcctgc ttatgctgca tcaaacccac gaatgccttt aactatatgg
    11041 agtgctggat gttcgagtgg agaagaacct tatacaattg ccataaccat tgaagaggct
    11101 aaacaaaaat atccgaatat tgattataca attttaggaa ctgatatttc aacgcgtatc
    11161 cttaagagcg ctcatgaagc catatacaaa gaaaacaggg tagaaggtat ccctatttca
    11221 attttaaaaa aatatttttt aagaagcaaa gaccgctcaa accctacagt acgtgttatc
    11281 cctgagcttc gtcgaaaggt ttctttcgga aggctcaatt ttatggataa ttattaccag
    11341 gtacaagaaa attttgacgt tattttttgt agaaacgtgt tgatatattt tgatagggag
    11401 acccaggaaa aggtaatatc caagctttgt tccaaactca aacccggagg gcatttcttt
    11461 ttaggccatt ctgagtcgat tacaggtatg cacctgcctt tgaagcaaat caagccaaca
    11521 attttctcta agattgaata attttattaa ctttgaataa attgcaaccc tgataaaaaa
    11581 atatttttat gagtaaactt acagaccagg aattaataga cgagttacaa cgcagatttg
    11641 aagaaaacaa aaagactatt caagagttga aagagcttac cgaagaactt aaaatagtta
    11701 acaaaaaatt agaagagtca gaggctttaa aaagtcattt tatatctaat ataacaaatg
    11761 aaataataaa cccttttacc tctatcttag gattatcaag aagtatctta tctgtaaaga
    11821 aagaaaactg gaagaaagtt atttctatgg ttgccctaat acattcagaa gcgtttaatc
    11881 ttgattttca gttgcgtaat atatttttgg cagccaaaat tgaagcaggt gaaatttacc
    11941 ctgaaatcct aaatgtagat ttgcggactt tagtcaagaa tgttattgac actttcagga
    12001 ttgaatcgaa gaaaaaaaat attgttatag aactgaattt tgatataaaa cctgccaacg
    12061 ggttagtttt ttattttaaa accgatcccg aaaaaataaa gctggcttta gctaacctgg
    12121 taagcaatgc cattaagttt agttacgagt caagcaaaat tattgttagg gtatggaaag
    12181 aaggagatac agtatttatg tctgttcagg actttgggac aggtatatcc gaagcaaacc
    12241 agaaaataat atttgacagg ttcaaacgct tagattcggg gattaattcg ttgaacaggg
    12301 ggcatgggct tgggttatct atcaataaag cagtaataga tttactacaa ggtaagataa
    12361 ccataaaaag tgaagtaaac aaaggggcaa catttaccat ttctgtgcca gaagccaaaa
    12421 ccaatattgc cggcttctct gccgatggaa atgagttttt gtttgacaat gaagaaatat
    12481 tttagattgc tggttgatgg aagagaatgt tcattttcta tatccggcat cgctttttgc
    12541 cagtaagtcg ccatatttgg tcacaactat attaggctct tgcgtggcag tttgcttgta
    12601 cgatgcaaat ttgcagattg gaggaattaa ccattatatg ctgcctttgt ggaatggtca
    12661 gggtttggct tctcctaaat acggtaatat agctatagag aagcttatag aaaaaatggt
    12721 ttcacttgga tgcgacagaa ataacctgaa ggctaaagtt tttggaggcg gggaggtcat
    12781 tgagactacg gttacgcagt ttaacattgg ggcacgcaat ataaagctgg cccacgaaat
    12841 gttagaagag ttaaaaatac caatagtagg tcaaagtgtt ggaggaaagc ttggtagaaa
    12901 aatcttattt aatactgaaa cctttgaagt aaaacagaag tttatacaaa aatcaaaccc
    12961 tttagaccat ttatagagta aacattaaag gaactatcat gaggcgaata aaggttttaa
    13021 tagtagatga ttcggctgtg gtgcgtcaaa cccttacaac aatacttgaa tcagactcac
    13081 agattgaagt aatgggagca gcctcagatc cgttttttgc cgcaaaaaaa atccagtcag
    13141 aagtacctga tgttattacc cttgacattg aaatgccaag aatggatggg cttactttcc
    13201 taaaaaaaat aatgtcacaa catcctattc cagtggttgt tatctcaagt ttaacttctg
    13261 agggtaccga aacagggatg aaagcccttg aatatggcgc ggtagaaatt attaccaagc
    13321 ctcagatgaa taccaagcag tttattgaag aatcaaggat acgtatttgt gatgctgtta
    13381 aagctgcagc cctttcaaaa atcaaacgta aaacatatag cgaaattact gttgccccta
    13441 agttatcagc tgatgctgta attcctattg ttaaggcccc gacagccatg ataaaaacca
    13501 cagaaacagt tgttgctgta ggagcttcta caggaggaac agaggcattg cagatctttt
    13561 tgcaaggaat gcctattgat tgccccggta ttgttatcgt tcagcacatg cctgaatttt
    13621 ttacccggtc gtttgccaat cgtttgaatg aaatttgtaa aataacagta aaagaagcag
    13681 aaaataacga tagtgttatc aggggcaggg cacttattgc ccccgggaac caccatatgc
    13741 ttttaaaaag aagcggggca agatattatg tagaggttaa agatggcccg cttgtaaaca
    13801 gacatcggcc atcagtagat gtattattca gatcaacagc ccgttatgca ggcaataatg
    13861 ccattggggt aattatgacc ggtatgggag atgatggggc ccgtgggctt ttggaaatga
    13921 aagaagcagg agcttcaact gtagcacagg acgaaaaatc atgtgtagtt tttggtatgc
    13981 ccaatgaagc cataaagctc ggagctgcag ataaaattat accattagac caaattgcag
    14041 cacaagtagt taaaatggtt gctttacgtg agaattcttg atctttaata aatgtatgac
    14101 cgccactatt atagatataa aaaatattac tatttttagt tgctcaaaaa tgatttacaa
    14161 ggccaatgtt aaagagaagc tataaatata ccatatgggg acttgtctcc gggatattct
    14221 ttattactat tctttggttt gccggctcta ttatcaataa tataccgttt tcgcttacct
    14281 catttgctta tctgcacaat atatacccta tcttttggta catagatttg ttgccattaa
    14341 taatgggttt gcttgccttt attatcgaaa aagaaaattt caatttcgaa attcttttag
    14401 aagaaaacct aaaacacgaa acccaaaaat cgcaaaatgt tttagacttt gtaaataacc
    14461 tgattcagga taatttcgat tttgaaattg aagatttaaa taatggcgat aaattgtcag
    14521 gcgccttggt cgaattacgt aacaaactaa agcaaaataa agccgaagag attattagaa
    14581 gaaaagaaga tgaccagaga aactggatat ccgaagggtt ggctaagttt agcgagttac
    14641 ttaggcgtaa taacaatgat cttaagcaaa tgtcttatga aattatcatt gaacttgtta
    14701 aatacattgg agccaatcag ggaggagttt ttcttcttga agaatccaaa agtggcgaaa
    14761 gatatttcat gcaaacagcg gcatatgcct atgaccgcaa aaaatttgcc aataaaagga
    14821 tagaatgggg cgaggggctc atcggtactt gtgcccttga acgtaaatcc atatatctta
    14881 cagaagttcc ggatagttac ctaaatataa catcaggttt agggaaagca aaccccagga
    14941 gcgtgttaat agttccattg cagttcaaca atgaaattca tggaattatt gaattagctt
    15001 cctttgaaaa gtttgaaaag tttgaaattg agtttgttga acgtatagct gaaagcacgg
    15061 catcaacatt agcaagtgtc aaaatcaacc tgcgtaccgc agacttgctc aaagaatctc
    15121 gagaacaggc tgagatactt gctgcgcaag aagaacagat gcgccagaat atggaagagc
    15181 tgcaggctac acaagaagaa gcagcccgtc agtcagaaaa atttattagt ttcaccaatt
    15241 ctgtaaacca tacccttatc agagctgaat atttagtgga tggcactttg atatatgcta
    15301 ataccaagtt tctcaacaaa ttgggatatg ccagcaacct tgaagtagaa ggcaagcata
    15361 tttcgatgtt tatcaacaaa aaagactgcg aatggtttga acctttatgg gaagaacttg
    15421 ccaacggtgg caaacacttt gaagggtata tgaagcacgt gaccaagctg gggcaagacc
    15481 tttggactat ggcaacctat acctgcgtca gaaaagaaga tggcaatgtt gagaaaatac
    15541 tttttctggc catagatacc acagagcaga aaaaacaaag ccttgactat gaaggccaga
    15601 ttgacgcttt gaaccgctcg agcataaaag ttgaacttca gccggaaggc aggattatga
    15661 gtattaatgc tcttttgacg gaatctttgg gttatacaga ccatgaaatg gttggtaaaa
    15721 agatgataga tttcatccat aaagaagatt tagacaattt taaagctatt tggaaaaatg
    15781 tgcttggagg tcagccattt atgggtcaaa tacgaaaggt tggcaaaaac aatgaagtaa
    15841 aatggtttag aggtacttat accgcggtaa tggatatgta cggtgatgta tcaaaggtca
    15901 tttttatagc caatgacatt accaaaaaaa agcaaatgga gatagaggct tcgcgtcaaa
    15961 atgagcagct caaagaacaa gaagaaaaac tcaggttggc aggagtcgaa cttagcctta
    16021 aattaaaaga ggctaaggaa gaaatgcgaa tgcaatttaa agaaatcgaa cgtataaaag
    16081 tgcggaacga acttaccctg gaaggggcat tggatgccat tattactatt aatgaagtgg
    16141 gtaccataga atttttcaac aaagcagctg aaatgctttg gggaattgac aggaaagacg
    16201 caattgggca aaatgttagc attttgtttg ataacgaaac tattgaaaat gatgaatttg
    16261 ttgcatcgta tgttaaaccg ggaacagata aaattgttgg agtgcgtaaa gaagttcaga
    16321 taaaaaccag agagggcgaa caaaaacctg tattattttt aatttcaatg gctaaagtag
    16381 ataatagtta tgcatatact gcatttatac aaaacattga agtagagctt ttttaataac
    16441 taaaaacaca tatttgaaca catcttcaga aaaagaacaa gggaaaagca aacagataga
    16501 tacttctgca ttaaagtttt cttttttgca ggataattcg ccgtactcta tttttgatta
    16561 tgaggttttt ttacattcat tgaaactttc aaaatctgca ataaccattt ttcgtccatc
    16621 tgggcaagag tttgttatta caaagtctat catcaatctg cttcaggtaa aaagtgatat
    16681 tgctataatc agggaatatt tccttagccg tggttttgat aaatatgacg gacagcttat
    16741 tatggctgcc attaataagg ttatagaaag tggcgaagcc accgaagaaa tgttgtatct
    16801 gaaagatagc agtttgcata aacgcaacaa ggtgtatcgc atattaatcc agccagctgc
    16861 attatggggg tttaataatt cggccgacct tattttgcac aacacagata tttccgaagt
    16921 gtctcatttg tacgatgatt tggccaaaga acaagaaaag gtgtctgaag ggattgtggt
    16981 gaaaaatgct tttttgaata atatatctca tgagatcaga gaaccgatga acaccatttt
    17041 gggttttaca gagcttctta cattaggcaa tattaaagac gaacagagga cagacttttt
    17101 gaatattgtc aaaaacaaaa gccgggcaat gctttcgatg atagatgata tttcggagtt
    17161 ggccaggttt gagtcggacg gtattactgt tgcaagaatg gaaaccaacg ttcctaagct
    17221 tttgaacgag ttgcttttag aataccataa ggagcttgac attaggcgaa aaagtaacat
    17281 agaactcata ttgaagttgc cttctgataa agattcttat gtaatatatg ttgattcggg
    17341 gcgtttgtat caggtgttaa attacctcct cgacaatgcc ctgaaatata ctgataaagg
    17401 ctttgtgcag tttgggtatg aagtaaaaga tgcaaaacag ttgcagtttt ttgtacgaga
    17461 tactggaggt ggcattccta aagacgaaca aaagtattta tttacccgtt atcgaaaaaa
    17521 agaagaagtg ctggataaac ctatattgaa gaaagggttg ggtttaacta tttcacgcta
    17581 tattgtagag ttgatgggag gcaaaatatg ggtggactca acagtaggcg agggtactac
    17641 tttttatttc acagtaccgt ttggaaagga aataaattca attgtttcag aagccaattt
    17701 tgtagaaaag ccttttttga attggcggaa caaggttata ctcattgtgg aagatgaagt
    17761 agtgaattac cagtttttag aagctgtgtt tgcagagtca caagctcaat tagtacatgt
    17821 tacagatggc gataaagctg ttgaattgtg taaaacattg aataatatag atttgatatt
    17881 aatggatatt cgtttgccgg tgaaaagtgg ttatgatgct gtgaaagaaa tcagacagtt
    17941 tagaccagcc attcctatta tcgcccaaac agcttattca agtaaaaaag aacgcgataa
    18001 atgcaaagcc gttggtttta atgagtatat aaccaaacct attgatattc cgttacttat
    18061 tcaaatgatt aataagtatt tcaattagta ctttctatcc attcaacaat tttcttgtcg
    18121 tcaggctttg ttatagaata atatactttt accagtttgc cgttttcatc tatcaggtat
    18181 ttttgaaaat tccatttaac aacagagctg aattgtccgt taagttcttt ctgcgttagc
    18241 cattggtata aaggatggat agagtcgcct ttaacattga ttttggccat catcgggaat
    18301 gttacgccga agttgatttt gcaaaagttt gcaatttctt cattagtgcc tggttcttgc
    18361 gaggcaaagt tgtttgacgg aaagccaata atgataaagt ttttgccgga atatttttta
    18421 taaagagctt ccaacccctc atattgtttg gtaaagccac actttgaggc ggtgtttaca
    18481 atcattactt ttttaccttt gagttgggca aagtcaaagg tgcttccatc aatggctaca
    18541 ggcttgaaat cgtaaaatgg tttaatgctt tgggcgttga caacgaatga ggttggggca
    18601 agtattgatg acaggaataa aaaaagcttc ttcataatgt ttttatttta taaacaatac
    18661 aaagaagctt tggtttactg aaagaagtat tcttagacta caatgtttac tatttttcct
    18721 gggacaacaa tcactttttt aggcgctttg ccttcgagcc aacgggcaga ttgttcgtgt
    18781 ttcattactt cggcttcaac cgttgcaacg tccatatttt taggtaaaga aagttcaaaa
    18841 cgccttttcc cgttaaatga aacggcataa acagcaaagt catcttccag gtattgcttt
    18901 tcagcttcag ggaaaggttc gtatgccaaa gttgtagaat ggcctaatat ttcccatatt
    18961 tcttcggcca ggtgaggggc aaaaggagca agcatttttg caaaagcttc gccggtagaa
    19021 gtggtaacct tttgtttttt tatgcacaag ttggtaaata tcatcatttg agagatggcg
    19081 gtgttgaaac gcagattttc aatatcacca cttatttttt cgatagatct gtgtagtaat
    19141 ttcaatactt caatatcctc agttccttgg aaatagttgt ttttatcgcc aaaaaagcgg
    19201 gaagcacggg tcaagaagcc aaatacgcct ttaactccat tctctgccca aggttttgtt
    19261 gcatccagag ggcctaagaa catttcgtac aaacgaagtg cgtctgcacc ataagtttgt
    19321 acaacatcat cggggttaac aacgtttttc agtgacttag acattttggc aatgatttgt
    19381 cgcagttctt ctccagtatc tttgtggaaa tattttcctt ctctttcttc aactaagtct
    19441 gaggggactt tagcgccagt catggtttcg taggcatagg ccaaaatcat cccctggtta
    19501 aatagttttt gaaatggttc gctggtagaa acgataccaa ggtcgaacag tactttgtgc
    19561 cagaaacggc tatataataa gtgaagtaca gcgtgttcag caccgccgac atataagtca
    19621 accggcatcc aatatttttc aagctttttg tcaaaaggag cagtatcatt ttctgggtcg
    19681 atataacgta agtaatacca gcatgagcca gcccactgtg gcattgtatt tgtttcgcgg
    19741 cgacctttgc gccctttgct gtctgttact tgtagccatt cacctgcatt ggcaaggggc
    19801 gattcgccat tttcgcctgg ctgatatttt tcaatattag gcaaagaaag tggtaattgg
    19861 gtatcgtcaa gtactgtaat ttctccatct tcccagtgga taacagggaa tggctcgccc
    19921 cagtagcgtt ggcggctaaa gagccagtcg cgcatacggt agttggtttt ttcagtgcct
    19981 ttacctgttt tttcaagcca tttgataatg gttttcaagc cattttcttt gtttagtttg
    20041 tttatatcta agccattatt ggcattagca gagtttatgt attctccgtc ttcagtccag
    20101 catgctttgc ctgccagtac ttgtgcacgg gtatcagcat cggctgttac agggtcaagg
    20161 atacagataa taggtatatt aaacttagtt gcaaattcaa agtcgcgtgt gtcatgggct
    20221 gggacagcca taatggcgcc ggtgccatat cccattagta cgtagtcggc aatccagatg
    20281 ggtattttgt tgccattgac agggttgatg gcataagtgc ctgagaatac gccagtcttt
    20341 tctttagcca gttcagttcg gtcgaggtca gattttaggc gtgctttttc aatataatct
    20401 tttacttcag cttttttatc agctgttgta atcgtatcaa ccattggatg ctcaggagct
    20461 actaccatgt aagtggctcc aaaaagagta tcaggccggg tggtaaaaac tttaattttt
    20521 tccggtttcc catcaatctc aaaattgact tcggcgccag tagagcgccc aatccaattg
    20581 cgttgcatct ctttaatacc ttcgggccaa tcaacagtgt cgaggccttg aagtaaacgt
    20641 tcggcgtaga gtgggatacg gagcatccat tgtttgaggt ttttgcgcaa aaccgggttg
    20701 ccgcattttt catggcttcc atctgttaat acttcttcgt ttgcacaaac aatctgacaa
    20761 tgagggcagt accaaacttg tgcttcggca tagtaagcca gtcttttgct ttcgatgtat
    20821 tcttttacgg ctttattgcc ctgacatttg atgtcatcag gtataggaag ttcgctgatg
    20881 gggcggcctt tttgttgttg ctcgtcaaac caggtattga aaagacggat gaagatccat
    20941 tgtgtccatt tgaaatactt tgggtcagtg gtatttattt ctctgtccca gtcataacta
    21001 agaccaagca tttttatttg tctgcggaaa ttgtcgcagt tacgattggt aacaatggca
    21061 gggtgagtgc cagtttgaat ggcgtattgt tcggcaggaa ggccaaaagc gtcccaaccc
    21121 atagggtgaa ggacattgaa gcctttcatg cgtttgtagc gcgctaatat atcggtagct
    21181 gtgtatcctt caggatgccc aacatgcaac cctgctccgg atggataagg aaacatgtca
    21241 agaatgtagt actttttggg gtttgaagtg tcgttactgg ttttgaatgt tttgtttttt
    21301 tcccaataag cttgccattt ggcttctaat tctttgaaat tgtattccat acgtaaattt
    21361 ttgcaaaggg caaaagtaag caaaattctc tttttactgc tattgaaaat gaggtggttg
    21421 ttgtattaag aaatgaaata tatttttgag ttatacatat atgttttcat gctcaattca
    21481 ttggaaattt tgatatatat ttatgacaat gaaacgcata agagaatata tcaaaaagta
    21541 ttatttgtcc tatatttcaa tagggtttga ctgctcagac aatccggttt atatccgtaa
    21601 agctaaaaat gccttattgg tgtttagcct gagcatattg cttattgttg ttcttatcgt
    21661 aaaggaattg caagagggaa aaacatgggt gatgccagtt catgctttaa ggatagttgc
    21721 tttgcttggt tgtatttatt tgttgtacag gaagtattat tatgcttctg tgaggttggg
    21781 gatttatgcc atttttattt cgtctataat tggcacttgt attgcgccgg ttcagaattc
    21841 atttcacttt ttgtcattct caatatgtgc tttgtctttt tatttcaaca acagacgggt
    21901 tgaaaatgtt gtgtttttta ttttgtgttt gttttatttt agtgtttccc agtattacaa
    21961 caattatcat ttgcctccgg ataattcggc ttcttatatt atttctttag taatcatatt
    22021 ttttattttt taccttacga cagccttgtt tgtgtctgag tataaatttt ttcagggctt
    22081 gataatgagc aaaaatcaag agttggaatc taaaaactcc gaccttgaat tgcaaaatcg
    22141 agagttggag aagttgaacc gttttaagaa tgattccatt tcaattatta gccatgattt
    22201 gaaaagtccg gtcaatgggc ttgttgagat gtgttatttg ttgaagaaaa attttaaaaa
    22261 ctataccccg gagaaatttg agcaaagaat tgagttgctc aaatataaca gcgagaaggt
    22321 aaataacatg ctgattgaac tgttggtatg ggcaaacagc cattcacacg ctattgaggt
    22381 gcagaaaaag ctggttgaag tgacagactt atgtaataat atcctggtta ataatcaatt
    22441 tggtgcttcg ctgaaaaatg ttgcactatc gctccaacaa tatgatagta aatacgaagt
    22501 agtgagcgac cctgtaatta ttagtattgt agtgaggaac cttattgcta atgctattaa
    22561 atactcaaaa tccgggggga gtattgaaat tgatataaaa aaagatgata atgctgtgtt
    22621 gatttctgtt atagacgatg gtattggtat ttctgagaat gtaattaagc tgattgaaga
    22681 aagggctgta ttgtcttcgt tgcctggaac taaagctgaa ataggccatg gaattgggct
    22741 ttctatttgt aaagacctgt gccggttaat tgatgcagac tttaggttaa aaaggaaatc
    22801 aaaagaagga gggacaatag ctacgttaat tattcctttt ggaagtttct tgtagtcata
    22861 taaaattgaa aatttataaa taggattgat tgcaaagaat tgagcaggtt gaaattactt
    22921 ttttagtaag tttgtaccgt ttcaaaatgt taccccttgc aaataagtaa attgattata
    22981 ttgtcgagcg tccttctttt ttctgccagg gctaaagctc aagactctat caggatagtt
    23041 gattttccta cgcagttaca tgcttttttc aaaatcaatt cttcaaacaa cgaaatagaa
    23101 atcagcaatc ccaatattag tgatgaattg aattatcgcc ctaatttggg aaccaatatt
    23161 caactgggtt tttcgtattc atggttaggc ttggcctatt ctatcatgct gccagccgac
    23221 aattcgatga ataaaaaata tggaaagacg agcggttttg tgctggaagg gcattttatg
    23281 aaaccccgga taatgataga tgtggcttat aaaaggttca agggttttta tttggctaac
    23341 ccgtcaagtt ttataaccgg atggaataat agccttgctt atcctcactc tgctgatttg
    23401 ggaacatcca tgttgtcgtt taattgtttt tatgttttta ggcctcagcg ttattcccct
    23461 acagcagctt atgcttttac aaaagctatg cgaaagcgcg gtggtagttg gatgttaggc
    23521 tgctttgggg ctaagagttt tatattttct gacagttcaa ttgtttccca gtcaatacat
    23581 caatatgtaa accctgaatt agatcttaaa gaaatggctt tttatgaatt tggcttatcg
    23641 tttgggtatt cgtatttgtt aactattttg aagaaaaatt ttgtatcgtt tacgttaatg
    23701 ccaggagttt cggttcagtc catctctcag aaatcggcca ttgacaatag tattaataac
    23761 cgaaatgcca tatcacaaaa aacggtattt agggtttcga tggggcgaaa tgggaataac
    23821 aactattatg gaatcgtcgc caatggagaa acttcagtag tgaaaaactc cagtactgaa
    23881 ttgagcttta tttcaaacta ttttttatgg acaccgcttt agtactgtaa actggaagtt
    23941 tatgaaaaag gtggacaaaa tcctgcatcc caagaggctc tcatttataa caggcaatgc
    24001 acctgcaaga gactgatgca ttaaaggcta actttcatag ccaaatttag ccaaacgaaa
    24061 atatccgaag gtaaatttcc agtccgcagc gaacagcgct tttcaccaag ccttacaatg
    24121 accatgtctt ttgaagggat tacaaatatg tattggccaa ggattccgcg ggcatatact
    24181 actctttcgc cattaaaaat ggtaatccac caatggtaac catagtagtc gagcgggccg
    24241 gtgccatatt cgttggtaag ccaactggca ggctctgaca gttctttaat ttataattct
    24301 gggatgggtg aaatccctcg cctttaggcg aagcattt
//
LOCUS       DAOSPF010000055        23452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2191_length_23452_cov_7.026798, whole genome shotgun sequence.
ACCESSION   DAOSPF010000055 DAOSPF010000000
VERSION     DAOSPF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_23452_cov_7.026798"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..3394)
                     /locus_tag="PK252_10740"
     CDS             complement(239..3394)
                     /locus_tag="PK252_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15227.1"
                     /translation="MKEYLRKLLQALNVEINEASIVTLLLLQSVFLGIFYGALDTASN
                     SLFLGVFPPDMLPKAFTISGVAGIIMTSIYSSLQSKMQFKNLALLNLFVVALLTSLLW
                     AGFSVTTSKWHVFALLVLMGPLNIVVMIGFWGTAGRIFSLRQGKRLFGLVDAGQIIGV
                     ILSSYAVPVILSFNIKTKDILLISAVSAMVALVFQLVISGQTSNLGGKAYKKEEKEPE
                     ESFLSLFKNPFVRLMSIFVALSMITAFFISFSFLAVTKAKYPDMDEMAKFLGVFIGTI
                     MFFTLLIKTLVYGRFMKTYGLRTSLVLSPAVLIVLSLVAVLIGVIGGYTTTAGGFIFF
                     FLIIALSRLFSVALKSSIEVPSFKILYQTVDVKIRHNVQARVDGTVNEISALTSGIVL
                     FALGSLSFFKLIHFTEVLVVLLVAWLFVAYKLYRAYKNNLDESLVKLKQHDKVEITKK
                     TTGIAATALKGDENTSKVMYIMNLQEKVQPLVYERLVPYLLNHKLEPVQKYSLERISN
                     LKLYEGIDAIKHIDNISFAAESKKIAGELTQELAEGFANERIVTLSRSKVASDRELAA
                     RLIGESRNAEYTHHLKFLLRDFDKQVKIAAIKASAKVKSAELCPVIAEFLQTDYGKYA
                     YDSLIEFGDIAIEHLEHLFSKSGNEDTLSYKIVRIIGKIGGDNATKSLVFKINYHDIH
                     IARLSAKLLLSLNYRVTDDSYYLFHQSLMHAINIEAWNIAARLSLKDSTDDVLLKEAI
                     GQEIHSGYEHIFTLLSLAYDAQTISHVRQNIESESAESTGYAMELLELVVSEDIKPTL
                     FPLIDDSSDSEKVKVLQNFFPVDRIPLNELLTDIVNRDCNQLNAWTKASALFYILKLE
                     NFLVSDDLVAQMFNPNELISELAALIVHKNAPERIENYYKRIDEKNRIRLNTSIKNYI
                     SEPEHLIINKMLYLKELDFLKSVPTDRLYYLAQHLEIEKYEDGADIEGVDEDFEVKVY
                     MLVEGKVKVQSFDGNEEIINQKTAFGGLFSHGYPMQIPSFCAIDNVVCYMVYQDLFDK
                     IIFDYPEFAEGLVMFNPIEKV"
     gene            complement(3426..4223)
                     /locus_tag="PK252_10745"
     CDS             complement(3426..4223)
                     /locus_tag="PK252_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOT15228.1"
                     /translation="MKNTPKKFDKELVLYIILAGFFLVNAMLAEVIGAKIFSLEKLFG
                     ISPLNIPFIGGEVFTLNLSVGILIWPFVFITTDIVNEYFGKSGVRRLSILGAILIAYG
                     FIIIFGGTELPPSDYWLQVNGFDKSGQPFDINYAYSTIFRQGMGIIVGSIIAFLVGQL
                     VDLYVFHFIRSKTGHKKLWLRANGSNLVSQFVDSFLILLIAFYWLGNWSFSKVIALGL
                     AQYIYKVGMAAALTPVIYWVHSAIDKYLGREHSEEMIDKAEEGLERT"
     gene            4670..7309
                     /locus_tag="PK252_10750"
     CDS             4670..7309
                     /locus_tag="PK252_10750"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOT15229.1"
                     /translation="MQGILNKTLVVIVGVVSLNVVAGEKPMYRANAEYYKRTYVTETL
                     KSAAPVIDGKLDDECWQHGSWSEQFVQQTPDEGGRASEITMFKVLFDKENIYVAFRCY
                     DSQPDKIQNLVGKRDELIGDMVGINFDSYFDKRFGYEFNITAGGSKIDQLLKNDGSAD
                     VNWNAVWYGATAIGDSGWTAEMQIPLSQLRYNDKPDQIWGLHAWRWISRHMEEDQWSL
                     IPRNNSGFIYSFGELHGLKDLQKSHSLELMPYILVSGKRSTIKLDNPFNKKYSGYGTV
                     GLDGKIAIGSDFTLNMTLNPDFGQVEADPSVMNLSAFETFLAEKRPFFLEGKNVMDFN
                     IDNDILFYSRRIGHQPMRIPDSDEGKKIYTSPPSNTSILDAVKLTGKTANGLSIGIMQ
                     STTNEEFTPIDSNKRQSNVLSEPYTNYFLARVQKDYKEGNTIIGGILTSTNRFTDNPR
                     LNNLAKDAYCGGLDFVQYLFNRNYVINAKGIFSYVQGDSSAITDLQRSAVHYFQRPDN
                     KNIHVDSTAKYLSGNGGSLSFSRTGKNKLRFEEQISWRSPGLDINDLGFINRADFVKQ
                     KARVWYNENVPNKVLRMYYIGFENEQIWNYEGIHEENSFLLDAYLNFINKWETGSWIY
                     RRQSNIDTRELRGGPAMRMNPFWHFGFNANTDWSKKFASNIHYTISHDFISGSNDFEW
                     EQGFYIKPINKFNIGAIYRYAENYYQKSWADDGKLNNETKYVMAKLVQKTMSFTLRLN
                     LNLTPDLSIQYYGNPFISNGSYSNYKLVTNAMASNYYDRFVNISDNDISRNADNSVLT
                     INENGQTYTIDNPDFNKREFHSNLVLRWEYRAGSVFYVVWSHQRSNSDRPYPSTLMSS
                     TKDLLDQNNENIIMFKLNYYLNL"
     gene            7362..8285
                     /locus_tag="PK252_10755"
     CDS             7362..8285
                     /locus_tag="PK252_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT15230.1"
                     /translation="MKPFVIAVFSACLLIEISAQKEIIKIWPDTVPNSLQNKNYKEQE
                     IEAWGRMGWARVSNPEIHIYKPSPSNNKHIAVILIPGGAYYRIAFEKEGIDMTKWLTQ
                     KGITAIILKYRLPNDSIMKDKAIGPLQDAQEAVRIVRRNAAHIGIAPNKIGVMGFSAG
                     GHLASTLCTRFDKKLYSSKDTVSARPDFAALIYPVISMKKELTHMGSMENLLGIIASD
                     SLIAAFSNELHVSRNTPPTFLVHSADDGAVNYLNSIEYFKALNKNNVKSELHIYPTGG
                     HGYGLAYQGQTEKNWTASFIEWLKSIENKSM"
     gene            complement(8433..9971)
                     /gene="rny"
                     /locus_tag="PK252_10760"
     CDS             complement(8433..9971)
                     /gene="rny"
                     /locus_tag="PK252_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT15231.1"
                     /translation="MIVTAGIAVAIGVVGGSILSYIAYMAILKKKKEKLLKEAEIEAE
                     VIKKDKILQAKEKFLQLKAEHEKTINERNNKMLQGENRIKQKEQAVSQKLEELQKQKR
                     ELETLKENLLVQTELIEKKQEELDKMHKAQVEKLESLSGMSGEEAKAQLVESLREEAK
                     TQAMSYINEIVDEAKMTANKEAKRIIINTIQRVATEAAIENSVTVFNIESDEIKGRII
                     GREGRNIRALEAATGVEIIVDDTPESIILSAFDPVRREVARLALHQLVTDGRIHPARI
                     EEVVQKVQKQIEEEIIEVGKRTAIDLGIHGLHPELTRLIGKMKYRSSYGQNLLQHSRE
                     VANLCAIMASELGLNSKLAKRAGLLHDIGKVPDDEPEMPHAIFGMKLAEKYKERPEVC
                     NAIGAHHDEEEMTTLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLENLALSYPGV
                     TKTFAIMAGRELRVIVGSEKVNDQDAEKLSFDIAKKIQDEMTYPGQVKITVIRETRAV
                     SFAK"
     gene            complement(10236..10541)
                     /locus_tag="PK252_10765"
     CDS             complement(10236..10541)
                     /locus_tag="PK252_10765"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT15232.1"
                     /translation="MDDKLSIKVNLVDRYYPLKVDRRDEEKIRKAAKLINEKVLQYKQ
                     RYNDNDKDMQDFLAMAALQFVIKNLELEDVFGEQVLNSKLSELNNDLEQFLKEERVL"
     gene            complement(10551..10844)
                     /locus_tag="PK252_10770"
     CDS             complement(10551..10844)
                     /locus_tag="PK252_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15233.1"
                     /translation="MQGDNDFVVNELQKKIDLLKSAYLKEKGISNELKKKVEELSASL
                     NEKVSEVEALEVKLSTLKLAKSITGNASDMHDAKVKVTNLVREIDKCIAQLNR"
     gene            11000..12691
                     /locus_tag="PK252_10775"
     CDS             11000..12691
                     /locus_tag="PK252_10775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT15234.1"
                     /translation="MPFNFKPFITLVFTFFFNTLFCQQKYNPPLATGLKISGGFADVR
                     NGTFHFGLDFSTEKKTGIPVLAINSGYLARIKVEPGGYGKALYIKHADGTMSVYAHLQ
                     SFNDTIDSIVKEIQYNSSSFAIDYTFDKTKILVKKSDTIAFSGNSGQSTGPHLHFEIR
                     DDKSQKPLNPLSFLKQIKDSIPPVIEKLLIYKPQKYINKLGLPYYKVTKKDTTGKPYI
                     KVPQNFGIGIDAYDNITDSSRKFAFYEAKLFFDNILIYHMKNDSLAFDNMASVKGIVD
                     YNQKTNFGKVYYYMFKFPCNQSIVNKYAFHDGLISLQDTFVHSLKVEVKDNSGNTASW
                     ITNIKADSALNKTELKGLENYKILCHQQKEIKYKEVMLIFPSNCLFTDVLIDIKQMKP
                     INSTLAPVVKVGDPGIPLYKAFYIYFDIKVIADSLKKKLVVVSLDENNKAYSIGGEIL
                     NNTIKASARSFGKFSIALDTVAPKIVPFNVNKNKLLNKDSISFEITDNLSGIEKYEVY
                     IDNKWALFEYDLKSNTIYFTNKQKKKNLFDNPEKLVITVWDKRNNYTTKQFNFAR"
     gene            12736..13413
                     /locus_tag="PK252_10780"
     CDS             12736..13413
                     /locus_tag="PK252_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15235.1"
                     /translation="MKKTVLFSAIILTFNTFISFSQKYSNNQFVVFSYSLDVDAKVKT
                     ELQPLENYIQVRTDKKQTKLDAMLVHSVYKIVTNLLADSLKIFFLPPNSFGDAIKYDD
                     YGYPNILIQKAIRLSDSKYFIKISVSIENSKYDEKGKKLQEGLFYPVVNVSLDIYNKF
                     GFNPIQTAEGQAVAVTPVNVTPDFIAGLEFVNLDKTVSTTNENLMSLIKRAMNEAIYS
                     IIYRKQK"
     gene            13425..14483
                     /locus_tag="PK252_10785"
     CDS             13425..14483
                     /locus_tag="PK252_10785"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOT15236.1"
                     /translation="MKSYKVVGVMSGTSLDGLDIALCEFSKNSHNWNGKILKADTIAY
                     SQQWRERLDNANQLSGYELIKLDTEFGHFIGTEIKKFLSKKKGKADFISSHGHTVYHQ
                     PAEKITFQLGNGAAIAAAASLTTISNFRVLDVALHGQGAPLVPIGDQMLFSDYDYCLN
                     LGGFANVSYSWYKKRIAYDVCPVNIVINRLAQQLGHEYDKDGELARQGKINDALLKEL
                     NKIDYYNTPAPKSLGREWVDDVFNGVLAKHKCSITDQLRTIYEHIAFQMYRVFSGTAS
                     KKILLTGGGTHNKFLVELIGNRIEYKLAIPEKEIIDFKEAYIFAFLGVLRMRQEYNCL
                     SSVTGARHDNVGGNIFII"
     gene            complement(14484..15653)
                     /locus_tag="PK252_10790"
     CDS             complement(14484..15653)
                     /locus_tag="PK252_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15237.1"
                     /translation="MANVDNLLVEPLVEIFFPEVGGTLTDQWHSCLQQVKHYQSQNNN
                     KAVIKVTCFVDVDSNEDYNNFVGLVQGDWERIEKFAPISVVSQAPYQHLISVEIISVS
                     TENVTKLEVCQCDNISYLKVASTEGEYFIISGLGEKRVETDIEKQAWAAFEKAEKILR
                     IEGLDFGDVIRQWNYIEKIVDYTGPNQHYQIFNDIRTEFYSKCEFKHGYPAATGIGMA
                     CGGVVVDLFAFKSNHTCQVIPVKSPVQYDAHKYTEKVLVAGSINKLPSSPKFERAKVL
                     QNHSGAIIFISGTAAIKGQDSDNILQVGKQSVNTVENIKKLTGIENLQQCKVMPGEIN
                     LSYLRVYVKKREMTTEVVRAVEDVIANNVKTLYLEADICRPELLVEIEGLGNIKN"
     gene            15752..17617
                     /locus_tag="PK252_10795"
     CDS             15752..17617
                     /locus_tag="PK252_10795"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT15238.1"
                     /translation="MIKKVPYYGRWIFFILLSAYIVHFIFFNINPIFIHSAQQTSFYI
                     NSSYFDNFLNYPGAIAEYCGSFIGSIYGIPFWGSLVAFAMIILSMIMFYLILSKIYKE
                     KLSFLWMAIPAFLMLSLMQDYYFPPSVFVKAFFLLVFVYFSASFNFNQYVKLFLILIL
                     SLSIYYTLGSTILVIYLISILCLDVVNSKQHLKTTLKGLFYIVLLFIIPYLGQKYFFN
                     IADERTWKDLVPVLPPIIKYTETYILNAFIFSLPVMIIFIALIKYAENKFHSEKQSQF
                     VQFINNYSIKPLLGFIVIVFIFLHFLPKQMDLSQKQKVLTAYFVSQEKWEEAINTAIE
                     SSDNYDYFININFNRAIYNNGQFANRILDYPQLLGISSFYPDANTTGQITIISSDLYY
                     ELGYIGEALHWAYEGLSILPWNQHLLKRLVMCNLIEGNTQAASTYLSVLKDAAWSDGF
                     YDKYIAYLSDTSLLSKDKEIAEKRQFMPNNSLIPNSITHKLKILLAKNNKNQRALEYL
                     AICYLLDHKIGDFMLLYPELKKIYPEKMPQIYELGVILYCYNTDRNLLNQFTFSKETQ
                     TYFVNYSKIMKKHKNNKSLAQIELKENYQNTYLYYLSFLSPLVTKMEIKKREVKN"
     gene            17628..19133
                     /locus_tag="PK252_10800"
     CDS             17628..19133
                     /locus_tag="PK252_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15239.1"
                     /translation="MTINTCKSTLLFLILFTLVSCNSIPTDYSKLEKTPAIYPDYTAI
                     TIPSNIAPLNFCIEEPGTKYCITFSSSKSKGFTILQKSNKVIIPQSKWERLLAENIGN
                     KILIDIYVYNNQWFKYQTIENTIAPEKIESHLCYRLINSVNILWREMGIYQRNLENYD
                     EKPIFLNRSTDLGCVNCHHFNKANPKEMTLHFRKYKVGTIITTKDSIIRLDTKTQYTM
                     SPFAYPAWHPDGKHVAYVVNRVRQIFTSTKSYHDVVFDEASDIVIYDIEKNTVTTSPL
                     LSSKNRENMPAFSPDGKYLFYISAAPKTSDSSEIYSKYDLLMAPYDANTNTIGQVDTI
                     LKSKEIDKSISYPRISPDGRYLLLTMCRYSYFPIFDKYADLYIFDLKSKKLSPLECNS
                     PYTDSYHTWSQTGRWIVFSSKRMDDAFTRPFIAYFDASGHAHKAFVIPQKNPAFYKTY
                     LKNYNLPEFINGEVNISDIQLRDLVQTNPKKVKFDEKIDIDGLSGASYIKK"
     gene            19167..20333
                     /locus_tag="PK252_10805"
     CDS             19167..20333
                     /locus_tag="PK252_10805"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT15240.1"
                     /translation="MKNKKFNWSTQTKKGLLAFKVITLSLIILGINSCKNDESLPEIA
                     PKSMFTFSFDPNNTKIVHFQNASKYINQDTKYYWTFGDGDTSIIENPVHTYANNGAYQ
                     VVLKVSNSSYSDSYYLSVVVSDKAVDSTSLIPVVSFVYSQKGRSIQFQNSSSGIDENA
                     TFKWTFGDTTSSNAENPTHIYNADGFYSVVLKVTQNYKSYSYAKTIFISEAEIPAFDT
                     LKPTASFSYYYQNNVVYFVNTSSNVTSNTKYTWNYGDGKPTENAENPNHTFVNGKYNV
                     VLKVTNSKYTVTSTQTINVPALQSDYDWSKKPVARFVYTREGKGVYFQNASSNITPDT
                     QYLWTFGTIENGLPATSTLENPYFVYSKPGTYSVVLKVTNANVSDSYPIEIVIP"
     gene            20333..21856
                     /locus_tag="PK252_10810"
     CDS             20333..21856
                     /locus_tag="PK252_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT15241.1"
                     /translation="MNIKTNKNWWPSFLTFWGFASFFYGLFVVSLNYTHFPFEGIKGF
                     ATIIAHWALITASSGIIIYCLFLNRWAFALLFPIFTTITGIIAYFIFQYDISINTAVI
                     QSTFNTNLDEASGQISLQLILYQFFIITISGFIAFRRFKVKLPKPYWVHWVIIILGLL
                     AIKCINNYRFNTIYQRNPFSLYLGLKNYQRDKLELSKTRIDISKDAECHEDSITVIVV
                     IGEALRADHVSLNGYYRSTFDKVKAFNPVSFPNIYSEWTHTLQSVPHILTRADSSNHK
                     PSSNETSFISVFRAVQFKTWWIGNQDLSLIALPFAKECDSIFINEPYKSDYSFTGKYD
                     GQLLPQISKAINSTANRKLIVVQQVGCHWWYPSYYPPEYEIFKPSIKTKSFNVEDKQK
                     IINAYDNAICYTDFFLSEILKKLSKQKAILFFLADHGELLGENGKWLHAQDTEYEKNP
                     ACFIWASEKYQKAFPEKMKALKTNQYKRFRTDFLFHSVLDAAEISLPLFQNTLSIFE"
     gene            21979..22908
                     /locus_tag="PK252_10815"
     CDS             21979..22908
                     /locus_tag="PK252_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15242.1"
                     /translation="MELSNTSQIELEDLDKSSIDTLLLIIDEHLGHSFDERIFSVKAK
                     QKFVLYLSDSSECIRCAKEKFIEHNFRLCNLPDNSYDSFIIEIQNICNERTSNKIKLL
                     IDNTLASSIWYGYILRFFANCELPMDTIEIYFSNSLIQSNVPLKSSKSKFTFNPLFIN
                     SNTNIEKRPLAIIVEQSFFNKEFIKLFENQFNPKYVIWFVEKRIADRITLNEQISREL
                     KCNVEIHNFLENKVEETDTALRQLVIRLRLNYKVSILTTHSKTFSLIASLINARYPDV
                     DIWQQEPEPVKISYEKTNSLIYKAVMINDELEY"
     gene            complement(22907..23341)
                     /gene="rnpB"
                     /locus_tag="PK252_10820"
     ncRNA           complement(22907..23341)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK252_10820"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         7850 a         4615 c         3724 g         7263 t
ORIGIN      
        1 caaaatagtc ttggtatgct gataatttat taatcccaaa ataatcaaag ctatcagcga
       61 cggaataatc acattataaa taagttgcct aaaaattgtc gttggttgtt tcatcatatt
      121 tatctttaat tcttaatgtt atgataactt gtcccgtctt tggcggaaaa actcgaatat
      181 tctcaattac tattgtgttt gcttatagta taacatgctc atttcataat tttagttttt
      241 acactttttc aattgggtta aacataacta agccttcagc aaattctgga taatcaaaaa
      301 ttatcttatc aaaaagatct tgataaacca tataacaaac tacattgtct atggcacaga
      361 aagaagggat ttgcatagga taaccgtgtg aaaacaagcc tccaaaagca gttttttgat
      421 tgattatctc ttcattgcca tcaaatgatt gaactttcac ttttccttca acaagcatat
      481 atacttttac ttcaaaatcc tcatcaacac cttctatgtc tgccccgtcc tcgtattttt
      541 cgatttctaa atgctgggca aggtaatata atcggtctgt cggtacagat tttaaaaaat
      601 ctaattcttt caaatacagc atcttgttta ttatcagatg ctctggctcc gatatgtaat
      661 ttttaatact tgtatttaac cttattcggt ttttctcatc tattcgctta taataatttt
      721 caattctctc aggagcattt ttatgaacaa tcaatgcagc cagctcactg attagctcgt
      781 ttgggttaaa catttgggca accagatcat ccgaaactag aaaattctcc aatttcaaaa
      841 tatagaacaa cgcactcgct tttgtccatg cattaagctg gttacagtca cgatttacta
      901 tatctgtaag aagctcattt aaaggaattc gatcaacagg gaaaaaattc tgaagaactt
      961 tgaccttttc actatctgac gaatcatcaa tcaaaggaaa taaagttggc ttaatgtctt
     1021 cagaaacgac aagctctaat aattccatag catatccggt actttcggcg ctttcgctct
     1081 cgatattctg cctgacatga gatattgtct gagcatcata ggcaagcgac aacagtgtaa
     1141 aaatatgttc ataacccgaa tgtatctctt gccctatcgc ttctttgagt aaaacatcat
     1201 cggttgaatc tttcaaactc aagcgggcag ctatgttcca tgcttctata ttgatagcat
     1261 gcatcaacga ctgatgaaac aaataatatg aatcgtctgt caccctgtaa tttaatgata
     1321 acaataattt tgccgagagt ctggcaatgt ggatatcgtg ataattgatt ttaaaaacaa
     1381 ggcttttagt tgcattgtct cctccaattt tccctataat cctcacaatt ttgtatgaaa
     1441 gcgtatcttc attccctgat ttgctaaata ggtgttcaag gtgttctatg gctatatctc
     1501 caaattctat taatgagtca taagcatact tcccgtaatc tgtttgtaaa aactcggcaa
     1561 taaccgggca aagctcagca cttttaacct tggcagaagc tttaatggca gcaattttaa
     1621 cctgcttgtc aaaatcgcgc aataagaatt tgaggtgatg agtatattca gcatttctgg
     1681 actctccaat aagcctggcc gccaactctc tgtctgatgc aacctttgaa cgggacagcg
     1741 taacaattct ttcatttgca aagccttcag ctagttcttg ggtcagctcc cctgctattt
     1801 tttttgattc tgctgcaaat gaaatattat caatatgttt aatagcatca ataccttcat
     1861 atagcttaag attagaaatc cgttccaaac tatatttttg tacaggctcc aatttatgat
     1921 ttaaaagata aggcaccaaa cgctcataaa caaggggctg caccttttcc tgcaagttca
     1981 tgatatacat tacttttgag gtattctcat ctcctttgag ggctgtagct gcaattcctg
     2041 ttgttttctt agtaatctca actttatcat gttgctttag ttttaccaac gactcgtcaa
     2101 ggttgttttt atatgccctg taaagtttgt aagcaacaaa tagccatgcc acaagcaata
     2161 ctaccaatac ttctgtaaaa tgtatcaatt tgaaaaacga taatgaacct aaggcaaata
     2221 agacaatccc tgaagtcaaa gctgatattt cattaaccgt accatcaacg cgggcttgca
     2281 cgttgtggcg tatcttaaca tctactgttt ggtacaaaat tttgaaagat gggacttcta
     2341 ttgagctttt taaagccaca gaaaacaaac ggctcaaggc aataatcaaa aagaagaaaa
     2401 taaaacctcc agccgtagtt gtataaccgc ctattacacc tatcaacact gcaactaatg
     2461 acaatacaat caatacagcc ggagatagca ccagactagt ccttaaccca tatgttttca
     2521 taaacctgcc ataaaccaat gttttgataa gcaaggtgaa aaacataatg gtacctataa
     2581 acaccccaag aaacttagcc atctcatcca tatctggata tttagccttt gtcactgcta
     2641 aaaacgaaaa actgataaaa aaggcagtta tcatggacag tgccacaaaa atagacatca
     2701 gcctcacaaa tgggttttta aataagctca agaagctttc ttcaggctct ttttcttcct
     2761 ttttataagc ttttccccca agattagaag tctggcctga tataacaagc tgaaacacca
     2821 aagcaaccat cgcacttact gcacttatca acaggatgtc tttagttttt atattaaaag
     2881 ataaaattac tggtaccgca tacgaactta atataacccc aataatttgc ccggcatcaa
     2941 ccaaaccaaa gagacgcttc ccctgtctta atgagaaaat acgaccagca gtaccccaaa
     3001 agccaatcat gacaacaata ttaagaggcc ccatcaatac aagcaatgca aatacatgcc
     3061 atttgctggt agtaacgcta aatccagccc ataataaaga tgttagcagt gccacaacaa
     3121 aaaggttcaa taaagccagg tttttaaact gcatcttcga ttgtaatgac gaatagatac
     3181 ttgtcataat aatacctgcc acccctgaaa tggtaaaagc cttcggtaac atatctggtg
     3241 gaaaaacccc taaaaacaat gagttggaag ctgtatcgag agctccgtag aaaataccaa
     3301 gaaaaaccga ttgcaatagc aacaaggtta ctattgaagc ttcattaatt tcaacattca
     3361 gggcctgtag taattttctt aagtattctt tcatttcagg ttagtaatca taaggattat
     3421 gtattttacg ttctttcaag accttcttcg gctttatcta tcatctcttc tgaatgctca
     3481 cggccaagat atttgtcaat agcagaatga acccaataaa taacaggagt aagagcggct
     3541 gccataccta ctttatagat atattgagct aatccaagag ctattacttt actaaagctc
     3601 caattgccta accaataaaa agctatcaat aaaatcaaaa aactatctac aaattgtgat
     3661 acaagattgg agccgtttgc cctaagccaa agctttttat gaccagtctt actacggata
     3721 aagtggaaaa cataaaggtc aaccaactgt cctaccagaa aagcaattat agaaccaaca
     3781 ataattccca tgccctgcct gaatattgtt gagtaggcat aattaatatc aaaaggctga
     3841 ccagacttat caaatccgtt aacctgaagc caataatcag atggagggag ctctgtccca
     3901 ccaaaaataa taataaaccc ataagcaata agaattgcac caagaatact taaccgtctg
     3961 accccggact tcccgaaata ttcgttcact atatcagtag taataaacac aaaaggccaa
     4021 atcagaatcc ctacactcag gttcagcgta aacacttctc ccccaataaa aggaatattc
     4081 agaggcgata ttccaaataa tttttcaagc gaaaaaatct ttgcaccaat cacttctgcc
     4141 aacatggcat tgactaaaaa gaacccggca aggataatat acaaaaccag ttccttatcg
     4201 aacttcttgg gggtattttt cattgtaatg tttttaggtt atatatcaaa ttattttatt
     4261 ttatcacata atagttacaa aatataagcg tatttactat ataactaata aaataaatta
     4321 ccaaagtaaa atattttcat gaatatacaa aaaatattta tctaacattt tttattttgt
     4381 ttagaaaata aagtatcata ataacctgca taaacaatca accttacata aaactttatt
     4441 aataaaatta ttctaaaatc aactaaccca cttcactttt acatcccccc aaaaaattca
     4501 cctctctttt tcgtttattc aacgcagttt tccctttttt caataataat taacacaaac
     4561 catgtttttt taatctgact tttgcataag ggtaaaatag ttgttagttg ttaattgtta
     4621 gttgttagtt attagttgaa acaaaattta ttaaaaaaac taagtattca tgcagggtat
     4681 tttaaacaaa acattggtag ttatagtagg ggtggtatct ttgaatgttg tggcggggga
     4741 aaaacctatg tatagggcaa atgccgaata ctataaaaga acatacgtta cagaaacatt
     4801 aaaaagtgcg gctcctgtta ttgatggaaa actcgatgat gaatgttggc aacacggctc
     4861 atggtctgaa caatttgtgc agcaaacacc cgatgaaggt ggccgcgctt cagaaattac
     4921 tatgtttaaa gtcttatttg ataaagaaaa tatatacgtt gcctttcgct gttatgactc
     4981 tcaaccagac aaaatacaaa atcttgtagg taaacgcgat gaacttattg gagatatggt
     5041 tggaataaac tttgacagct attttgacaa gcgttttgga tatgaattca atattaccgc
     5101 cggaggttca aaaatagacc aattattaaa aaatgacggc tcggccgacg taaactggaa
     5161 cgctgtttgg tatggcgcta cagctattgg agatagcgga tggactgcag agatgcaaat
     5221 cccgctaagc caactaagat ataacgataa accagaccaa atatggggct tgcatgcatg
     5281 gcgatggata tcacggcaca tggaagaaga ccaatggagc ctcatccccc gaaacaacag
     5341 cggattcata tattcatttg gggagctgca cggtttgaaa gacctacaaa aatcacacag
     5401 cctggaattg atgccctata tattggttag tggcaaacgc tctacaataa agcttgacaa
     5461 tccttttaac aaaaaatact caggttacgg gactgtaggt ctggacggaa agattgccat
     5521 tggaagcgat tttaccttaa acatgacgct taaccctgat tttggccagg ttgaagcaga
     5581 cccttctgtg atgaacctga gcgcttttga aactttcttg gcagaaaaaa ggcctttttt
     5641 tctcgaagga aagaatgtca tggatttcaa tatcgacaac gacattctct tttattcacg
     5701 aagaattgga catcagccca tgcgcatacc cgatagtgac gaaggaaaga aaatctatac
     5761 ctctccccct tcaaatactt ctatccttga tgccgtaaaa ctgacaggaa aaacagcaaa
     5821 cggcctgtct attgggataa tgcaaagcac aacgaatgaa gaattcaccc cgattgattc
     5881 aaacaaaaga caaagcaatg tgctgtcaga accttataca aactattttc tggccagggt
     5941 tcaaaaagat tacaaagagg gtaataccat tatcgggggt atcctgactt ccaccaacag
     6001 gtttacggat aaccctcgtc tgaacaacct tgccaaagat gcttattgtg gaggcctcga
     6061 ttttgtccaa tatttattta acagaaatta cgtaataaat gctaaaggca tatttagtta
     6121 cgtccagggg gactcttctg caattaccga tttgcaaaga tctgctgtcc actactttca
     6181 aaggcctgac aataaaaata tacatgttga ttctacggcc aaatatcttt ctggcaatgg
     6241 agggagcttg tcattcagcc gtacaggcaa gaataagctt aggtttgaag agcaaatctc
     6301 gtggagaagc ccgggactgg acatcaacga tttaggcttt atcaaccggg cagattttgt
     6361 gaagcaaaaa gcccgcgttt ggtacaacga gaatgtgccc aataaagtcc ttcgtatgta
     6421 ttatattggc tttgaaaacg aacaaatatg gaattacgaa ggcatacacg aagaaaattc
     6481 atttctgctt gacgcatact taaactttat caataaatgg gaaaccggct cttggattta
     6541 ccggagacaa agtaatattg acacccggga actcagggga ggaccggcta tgcgtatgaa
     6601 ccctttctgg cattttgggt tcaatgccaa caccgattgg tcaaaaaaat ttgcctcaaa
     6661 tatccactac acgattagtc acgattttat aagtggttcc aacgattttg aatgggaaca
     6721 gggattttat atcaaaccta tcaacaaatt caatattggc gcaatatatc gttatgcaga
     6781 aaattattat caaaaaagct gggctgacga tggcaaacta aataacgaaa ctaaatacgt
     6841 gatggcaaaa cttgtacaaa aaactatgag ctttaccctg cggctaaacc taaacttaac
     6901 gcccgactta tctattcaat actacggcaa cccattcatc tcaaatggca gttatagcaa
     6961 ttacaagctg gttaccaatg ccatggcaag caactactac gaccgttttg taaatatcag
     7021 tgataacgac attagccgca atgcagataa tagcgttttg accattaatg agaatggcca
     7081 aacctatacc attgacaatc cggatttcaa caaacgggaa ttccattcaa accttgtact
     7141 gcgctgggaa tatcgggccg gttcggtatt ttacgttgtc tggagccatc agcggagcaa
     7201 ttcggacagg ccttaccctt ccacgttgat gtcttcaaca aaagacctgt tggaccaaaa
     7261 caacgaaaat atcatcatgt tcaaactgaa ctactatctg aatttgtagt acccacaaca
     7321 tttattagct ttgctttaca attcatataa attgcaccac tatgaaacca tttgtaatag
     7381 cagttttttc agcatgttta ttaattgaaa tttctgcaca aaaagaaatt ataaagatat
     7441 ggcctgatac tgtacccaat tctttacaaa ataagaatta taaagaacaa gagatagaag
     7501 cctggggaag gatgggctgg gcaagagttt caaaccccga gattcatatt tataagccat
     7561 ctccatcaaa caataaacac attgcagtaa tactcattcc cggaggagct tattatcgta
     7621 tagcctttga aaaagaaggg atagacatga caaaatggtt aacccaaaaa ggcattacag
     7681 cgattatcct caaatacagg ctcccaaatg acagtatcat gaaagataaa gctattgggc
     7741 ctttgcagga tgcacaggaa gctgtccgaa ttgtcaggcg caatgcggca cacattggca
     7801 tagcccccaa caaaatagga gtaatggggt tttccgcagg aggccattta gcttcaacct
     7861 tgtgtacgcg ttttgacaaa aaattgtaca gctcaaaaga tacagtaagc gcccgccctg
     7921 attttgcagc tcttatttac cccgttattt ctatgaaaaa agaacttacc cacatgggtt
     7981 ctatggaaaa ccttttgggt ataattgcca gtgattctct tatagctgca ttttctaatg
     8041 aattgcacgt ttcaagaaat acaccaccaa cattcctggt acattcagca gatgatgggg
     8101 ctgtaaatta tcttaacagc attgaatact tcaaagcgct aaacaaaaat aacgtaaaat
     8161 ctgagctaca catttatcct accggaggac atggctatgg attagcttac caaggccaaa
     8221 ctgaaaaaaa ctggactgcg tcatttattg aatggctcaa aagtattgaa aataagagta
     8281 tgtaaaaatc aaaaaactaa ccgcaaaggt agccaggttt atcgcaaaga acacaaaatg
     8341 ttaataatca acaatcaaaa ttttgcgtac ttgatttctt tcttggcaaa ctttacggtt
     8401 agtttataca cctttttatt tataatccat tattattttg caaagctcac tgccctcgtt
     8461 tcgcgaataa cagtaatctt tacctgaccc ggatacgtca tttcatcctg aattttctta
     8521 gcaatgtcaa atgaaagctt ttcggcatcc tgatcattaa ctttttcgct acctacaata
     8581 acacgcaact ctctaccagc cataatagca aaggtttttg ttacacccgg atatgaaagg
     8641 gcaaggttct caagatcttt caaacgtttg atgtatgctt caacaatttc gcgacgtgcc
     8701 cctggccttg cccccgaaat ggcatcacaa acctgcacaa taggagcaat caatgttgtc
     8761 atctcctctt catcgtggtg agctccaatg gcattgcaaa cttcaggtct ttccttatat
     8821 ttctcagcca gtttcatacc aaagattgcg tgtggcattt ctggttcatc atctggaact
     8881 tttccaatat catgcaacaa tccggcacgt tttgcaagtt ttgaattaag cccaagctct
     8941 gatgccataa tagcacatag gtttgcaact tcacgtgagt gttgtaacaa attttgccca
     9001 taagatgaac gatatttcat cttaccaatt agcctggtaa gctctgggtg taaaccatga
     9061 atacccaaat ctatagccgt acgtttgcca acttcaataa tttcttcttc tatttgtttt
     9121 tgaaccttct gaaccacttc ttctatacgg gcaggatgaa tacgaccatc tgtaaccagt
     9181 tggtgcaaag ctaaccttgc aacctcgcgt cttaccgggt caaaagctga aagtattata
     9241 gattcaggag tatcgtcaac aattatttct acaccggtag ctgcttcaag cgccctgata
     9301 tttctgcctt cacgacctat aatacggcct ttaatttcat cgctttcaat attaaaaact
     9361 gtaaccgagt tttcaatagc tgcctctgta gctacacgct gaatagtatt tattataata
     9421 cgtttagctt ctttattagc agtcatcttg gcttcatcta caatctcgtt gatataagac
     9481 attgcctgtg ttttagcttc ttcacgtaac gactcaacca actgagcttt agcctcctct
     9541 ccagacatcc ctgaaagaga ttcaagcttc tcaacctgag ctttatgcat cttatcaagc
     9601 tcttcctgtt tcttctctat caattcggtc tgaaccaaaa ggttttcttt cagtgtttct
     9661 aactctctct tctgtttttg caactcctca agtttttgcg aaacagcctg ctccttttgt
     9721 ttgatacggt tttcaccttg aagcatttta ttatttcgct cattgatagt tttctcatgt
     9781 tctgctttta actgaagaaa cttttctttt gcttgcaaaa tcttatcttt cttgataacc
     9841 tctgcttcta tttctgcttc tttaagaagt ttctccttct tttttttcaa tattgccata
     9901 taggcaatat aactcaaaat acttcccccc acaaccccaa tagccacagc tatacccgct
     9961 gttacaataa tggaggttat aattgtcaat atgttcataa tttataaagt tttatatatt
    10021 aaaataaaaa ggcccgcagc tattccttca taagggtaag aaaccccaaa gtgttacgtt
    10081 tggagttgcc gcttgtattc aaacttccac cgtcatcctg agatgaaccc tgacttgcag
    10141 ggcgaggcct gttctacaac aaacgagcaa tactcgtaat ggcatttgtg ttgagtttca
    10201 cgccaattga agaaataatg cgggctttat ctttgttaaa gaactctctc ctcttttaaa
    10261 aattgctcca ggtcattatt tagttctgac agtttggaat ttaaaacctg ttctccaaaa
    10321 acatcttcca actccaaatt ttttattaca aactgcaatg ctgccatagc caaaaagtct
    10381 tgcatatctt tgtcgttatc attgtaacgt tgcttatact gcaacacttt ctcattaatc
    10441 agtttagcag ccttgcgaat tttctcttca tccctcctgt caacctttag tggataatac
    10501 ctgtctacca aattaacttt tatggataac ttatcgtcca ttaaaatatt ttacctgttt
    10561 aattgcgcaa tacatttatc tatttcccgc acaagattgg taacttttac cttggcatca
    10621 tgcatatcag acgcattgcc tgttatagat ttagccaact tcaaagtact caatttaact
    10681 tccaatgctt caacttcact aactttttca tttagcgatg ctgaaagttc ttcgactttt
    10741 tttttcaact cattgctaat gcctttttct ttcagatatg ctgactttaa aaggtcaatt
    10801 tttttttgca actcattgac tacaaaatca ttatcacctt gcatggtcgt tgtttttcct
    10861 tttacaaata taatacagct tttatatttt atactaaatt caatgttttt tatactataa
    10921 acgttttttg tgcttttgtg ttctcttcaa actgtatatt ttcaaagata agtttcttag
    10981 atttgcaaaa aaatgcatta tgccctttaa ttttaaacct tttataactt tagtttttac
    11041 gttttttttt aacactcttt tttgtcagca aaagtataat cctccattag ccacaggctt
    11101 gaaaatttcc ggaggctttg ctgatgtcag aaatggcaca tttcattttg ggctggattt
    11161 ttctaccgaa aaaaaaaccg gaatacctgt attggcaata aatagtggat atctcgcccg
    11221 cataaaagtt gagcccggag gttatggaaa agccttatat ataaaacatg cagatggtac
    11281 aatgtctgtg tatgcccatc tgcaatcatt taacgatact attgacagca ttgtaaaaga
    11341 aatccaatac aacagctctt catttgctat tgattacact tttgataaaa ctaaaatatt
    11401 ggtaaaaaaa agcgacacta ttgcattttc aggaaattca gggcaatcaa cagggccaca
    11461 cctgcatttt gaaatacgcg atgacaaatc gcaaaagcca ttaaatccgc tttctttttt
    11521 gaaacaaatt aaagacagta tccctcctgt tattgaaaaa ctcctgattt ataaacctca
    11581 aaaatatatt aacaaattgg gcctccctta ttataaagta acaaaaaaag atactacagg
    11641 aaaaccttac atcaaggtcc ctcaaaactt tgggattgga attgatgcgt atgataatat
    11701 tacagacagc tcaagaaaat ttgcttttta cgaagccaaa cttttttttg ataatatact
    11761 gatttatcac atgaaaaacg actcgctggc ttttgacaat atggcttctg taaaaggcat
    11821 tgtggactat aatcaaaaaa caaacttcgg aaaagtatat tattatatgt ttaagtttcc
    11881 ttgtaaccaa tcaatagtaa ataaatatgc ttttcatgat gggctgatta gcctgcaaga
    11941 tacatttgtt cattctttga aagtagaagt taaagacaat agtggcaata ctgcttcatg
    12001 gataacaaat attaaggcag attcagcatt aaacaaaaca gagttgaaag gcttggaaaa
    12061 ttataagatc ttatgccacc agcaaaaaga aataaaatat aaagaagtaa tgcttatttt
    12121 tccttcgaat tgtttattta ctgatgtttt gattgatata aaacaaatga aaccaataaa
    12181 ttcaacattg gcacctgttg taaaagttgg tgaccctggc attccactct acaaagcttt
    12241 ttatatttat tttgatataa aagttattgc tgacagcctc aaaaaaaagc ttgtagtagt
    12301 ttcgcttgat gaaaataata aggcctacag cattggagga gaaatactca acaacacaat
    12361 taaagcaagt gccaggtctt ttggcaaatt tagcattgct ttagatactg tagcccccaa
    12421 aatagtacct ttcaatgtta ataaaaataa acttctcaat aaagacagta ttagttttga
    12481 aataacagat aacctctcag gcattgaaaa atatgaagtt tatatagata acaaatgggc
    12541 tttgtttgaa tatgacctga aatccaacac catatatttt acaaacaagc agaaaaaaaa
    12601 gaatttgttt gacaatcctg aaaaattagt tataacagta tgggataaaa gaaataatta
    12661 cactacaaag caatttaatt ttgcacgata acatgttttt tataccttag ccctcaaaac
    12721 ttaactaaac ttacaatgaa aaaaacggtc ttattctctg ctattatttt aacattcaac
    12781 actttcattt cattttcgca aaaatattcg aataatcagt ttgtagtatt ttcatactcc
    12841 ttagatgttg atgccaaagt taaaacagaa cttcaacctc tcgaaaacta catccaggta
    12901 aggactgata aaaaacaaac caaattggac gctatgcttg ttcactcggt ttataaaatt
    12961 gttacaaacc ttttagctga ttccctgaaa attttcttcc tccctcccaa ttcatttgga
    13021 gatgctatca aatatgacga ttatggctat ccaaatattt taatccaaaa agcaatcagg
    13081 ttatcagaca gcaagtattt tataaaaatt agtgtatcaa ttgaaaacag caaatatgac
    13141 gaaaaaggga aaaaattgca ggaagggctt ttttacccgg tagtcaacgt gtcgttagat
    13201 atatataaca aatttgggtt taaccccatt caaacagctg aaggtcaggc tgttgcagta
    13261 acccctgtta atgttactcc tgattttatt gccggtcttg aatttgtcaa tttagacaag
    13321 accgtaagca ctaccaatga gaaccttatg tcattaatca aaagggctat gaatgaagct
    13381 atttacagta ttatttatcg aaaacaaaaa taatacttac agccatgaaa agctataagg
    13441 tggttggtgt tatgtctgga acttcgcttg atgggctgga cattgcattg tgcgaatttt
    13501 caaaaaattc acataattgg aacggcaaaa tattaaaagc agacaccatt gcatatagcc
    13561 aacaatggcg tgaacgcctg gacaatgcca atcaactctc gggatatgaa ctaattaagc
    13621 ttgatacaga atttgggcac tttattggca ctgaaattaa aaagttctta tcaaaaaaaa
    13681 aggggaaagc cgattttatt tcttctcatg gacatactgt ttaccatcaa cctgctgaaa
    13741 aaataacctt tcagcttggc aatggggccg caattgcagc agcagcctct cttaccacca
    13801 taagcaactt cagggtgctg gatgtagctt tgcatggaca aggggcgccg ttagtcccca
    13861 ttggcgacca aatgcttttt agcgactatg attattgcct gaaccttggt ggttttgcaa
    13921 acgtatcgta cagctggtat aaaaagcgaa ttgcttatga tgtttgtcct gtaaatattg
    13981 ttatcaaccg ccttgctcaa caattagggc atgaatacga caaagatggc gaacttgcca
    14041 gacaaggtaa gataaatgat gcactgctaa aagaattaaa taaaatagat tattacaata
    14101 ctcctgcccc aaaatcgctt ggcagagaat gggtagatga cgttttcaat ggtgtacttg
    14161 ctaaacacaa atgctctata accgaccagc ttaggactat atacgaacac atagcatttc
    14221 aaatgtaccg tgtattctct ggtactgctt caaaaaagat tcttttaacc ggaggcggta
    14281 cacacaacaa gtttttggtt gagcttatcg ggaaccgtat tgaatacaaa ttagctattc
    14341 cggaaaaaga aattattgat tttaaagagg catatatctt tgccttctta ggtgtattaa
    14401 gaatgagaca ggaatacaat tgcctatcat ctgtaactgg cgccagacat gacaatgtag
    14461 gaggcaatat ttttataata taatcaattc tttatattac caaggccttc tatttcaaca
    14521 agcaactcgg gcctgcaaat atctgcctca agatataaag tcttaacatt attggctatc
    14581 acgtcttcta ctgctcttac aacctcagtt gtcatctctc ttttctttac ataaacacgc
    14641 agataactta ggtttatttc tccaggcata accttacatt gctgaagatt ttcaattcct
    14701 gtcaacttct ttatattctc tacagtatta acactctgct taccaacctg caaaatatta
    14761 tccgaatcct gacctttaat tgctgctgta cccgaaataa atattatcgc ccccgaatgg
    14821 ttttgtaaca ccttagcccg ttcaaacttt ggcgatgaag gtaatttatt tattgaaccg
    14881 gcaacaagaa ctttttcggt gtacttatgg gcatcatact gaacagggct tttgaccgga
    14941 attacctggc atgtatgatt acttttaaac gcaaacaaat caacaacaac acctccacat
    15001 gccataccta tcccggttgc cgcaggataa ccatgcttaa attcacattt tgaataaaac
    15061 tctgtccgaa tatcgttaaa aatttggtaa tgctggttcg gtccggtgta atccactatt
    15121 ttttcgatat aattccattg cctgattaca tctccaaagt cgagcccctc tattcttaat
    15181 attttctccg ctttttcaaa agcagcccag gcttgctttt caatatctgt ttcgaccctt
    15241 ttctcaccca accctgaaat gataaaatat tcgccttctg tagatgctac cttcaaatat
    15301 gaaatattat cacattgaca aacttccagt ttagtaacat tttcggtact tacagaaata
    15361 atttctactg aaattaaatg ctggtaagga gcttgactaa ccacactaat tggtgcaaat
    15421 ttttcaatac gctcccaatc tccctgaacc agcccgacaa aattgttata atcttcatta
    15481 ctatcaacat ctacaaaaca agtaactttt ataactgctt tattattatt ttgagattga
    15541 taatgcttaa cctgttgaag acaagaatgc cattgatctg ttaaagtacc gccaacttca
    15601 ggaaaaaaaa tttcaacaag tggttcaacc agaagattat ctacattagc cataaaaaaa
    15661 aacctctctc agatagatat tatcaacaat attattataa ttgtgtcgcc caaaagtaat
    15721 aatattttta ttctataaaa ataaagttga tatgataaaa aaagtaccat attacggcag
    15781 atggatattc tttatcctac tttctgctta tatcgttcat tttatttttt ttaatatcaa
    15841 tccgattttc atccattcgg cacagcaaac ctcattttac ataaacagct catattttga
    15901 caatttttta aattaccccg gagcaatagc cgagtattgt ggatcattta taggctctat
    15961 ttatggaata cctttctggg gaagccttgt tgcctttgcc atgattatat tgtccatgat
    16021 catgttttat ctaattcttt caaaaattta taaagaaaag cttagctttt tgtggatggc
    16081 tatcccggct tttctgatgt tgagtttaat gcaagattac tattttccgc cttctgtttt
    16141 tgtcaaagcc ttttttctac ttgtcttcgt atatttttct gcatcattca atttcaatca
    16201 atatgtaaaa ctgtttctaa tattaatact ttctctatca atatattaca ctcttggcag
    16261 tactatactt gttatttacc tcataagcat attatgtttg gatgttgtaa attcaaaaca
    16321 gcatctaaaa accacactca aagggctatt ttatatagtt ttattgttta tcatccctta
    16381 tttgggccag aaatactttt tcaacattgc agatgagagg acatggaaag acttggtacc
    16441 tgtactccct ccaataatta aatataccga aacctatatt ttaaacgcat ttattttttc
    16501 attgccagta atgattatct ttattgcatt gataaagtat gccgaaaaca agtttcacag
    16561 cgagaaacaa agccaattcg tccagtttat caacaattac agcattaagc cgcttttagg
    16621 ctttattgtt atagttttta tttttttgca tttccttcct aaacaaatgg atttaagcca
    16681 aaaacaaaaa gtattgactg cttattttgt ttcgcaagaa aaatgggaag aagctataaa
    16741 tacagctatt gaatcatctg ataactatga ttattttatc aatatcaatt tcaacagggc
    16801 tatatacaac aacgggcaat ttgcaaacag gatactggat tatccgcagc ttttgggcat
    16861 ctcttcattt tatcccgatg ctaatactac aggccaaatc actattattt caagcgattt
    16921 gtattacgaa ttaggctaca ttggcgaagc attgcattgg gcatacgaag gattgtctat
    16981 tttaccctgg aaccagcatt tactgaaaag gttagtcatg tgcaacctga ttgaaggcaa
    17041 tacccaagct gcatcaacct atctttctgt attgaaggat gcagcatgga gtgatggatt
    17101 ttatgataaa tatattgcat atctctcaga cacatcccta ttatcaaaag ataaggaaat
    17161 tgcagagaaa agacagttta tgccaaataa ttctttaatt cccaactcta ttacgcacaa
    17221 actaaaaata ttgctggcaa aaaacaataa aaaccaaagg gctcttgaat atcttgcaat
    17281 ttgttactta ttagaccaca aaataggcga ctttatgctg ctatatcccg aactgaaaaa
    17341 aatatatccg gaaaaaatgc cccagattta tgagcttggg gttattttat attgctataa
    17401 cactgacaga aatttgttaa accaatttac attcagtaaa gaaacccaaa catattttgt
    17461 caattacagc aaaatcatga aaaaacataa aaacaacaag tcgttagcac aaattgaact
    17521 taaagaaaac tatcaaaaca cttatttgta ttaccttagt ttcttgagtc ctctggtaac
    17581 aaaaatggaa ataaaaaaac gtgaagtaaa aaactaaaat tcaagggatg acaataaata
    17641 cctgcaaatc aacacttttg tttctgatac tatttacatt ggtatcatgt aactctattc
    17701 ctactgatta ttcaaaactt gaaaagacac ccgctattta tcctgattac actgcaataa
    17761 caataccctc aaatattgca ccattaaatt tttgtattga agaacctggg acaaaatatt
    17821 gtataacatt ctcttcttca aaatcaaagg ggtttaccat tcttcaaaaa tctaataaag
    17881 ttattatccc ccaaagtaaa tgggaacgtt tgcttgcgga aaatattggc aataaaatac
    17941 ttatagacat ttatgtatat aacaatcaat ggtttaaata tcaaacaata gaaaatacca
    18001 ttgccccgga aaaaattgaa agccatttgt gctacaggtt aataaacagt gtaaatattc
    18061 tgtggagaga gatgggcatt tatcagcgaa acctggaaaa ttatgatgaa aaaccaatat
    18121 ttctaaatcg ttcaacagat cttgggtgcg tgaattgcca tcatttcaac aaagccaatc
    18181 ccaaagaaat gacccttcat tttagaaaat ataaagttgg aactattata actacaaaag
    18241 attcaataat caggcttgac acaaaaacac aatacaccat gtctcctttt gcctatcctg
    18301 cctggcatcc tgacggaaaa cacgttgctt acgttgtaaa cagggtaaga caaatattta
    18361 cttctaccaa aagttatcat gacgtagtat ttgatgaagc gtcagatatt gtaatttatg
    18421 acattgaaaa aaacaccgtt accacatctc cattattatc aagcaagaac agggaaaaca
    18481 tgccagcttt ttcaccagat ggcaaatacc ttttttacat ctcagctgcc cccaaaacct
    18541 ctgattcaag cgaaatatac tcaaaatacg accttttgat ggctccatac gatgcaaata
    18601 ccaatactat aggacaagtt gacactattt taaaatctaa agaaatagat aaaagtattt
    18661 catacccaag aatatcgcca gatggacgat atttgctttt gacaatgtgc aggtacagtt
    18721 acttcccaat ttttgataaa tacgcagatt tgtatatttt tgacctaaaa tcaaaaaaac
    18781 taagcccatt ggaatgcaac tcgccatata ccgacagcta ccatacatgg tcgcaaactg
    18841 gccgctggat agtttttagc tctaaaagga tggacgatgc ttttaccagg ccgtttatcg
    18901 cctacttcga tgccagtggc cacgctcata aagctttcgt tatacctcaa aaaaatccgg
    18961 cattttataa aacatacctg aaaaattata atctgcctga atttatcaat ggagaagtta
    19021 acatttctga tattcagttg cgtgatttag tacaaacaaa tccaaaaaaa gttaaatttg
    19081 atgaaaaaat tgacattgat ggactttcag gagcttcata tataaaaaaa taaataaaca
    19141 ataattaccc ttaaaaaaaa atcatgatga aaaacaaaaa attcaattgg tcaacacaaa
    19201 caaaaaaagg acttttagct tttaaagtta ttacattaag tcttatcatt ttaggtatta
    19261 atagttgcaa aaacgacgag tcgttgcctg aaattgcacc aaagtcaatg tttacattct
    19321 cttttgaccc taacaatacc aaaatcgtcc atttccagaa tgcaagtaaa tacattaacc
    19381 aagataccaa atattattgg acatttggag atggagacac ttcaataatt gaaaaccctg
    19441 tacataccta tgcaaacaat ggagcctatc aggtagtact taaagtatcc aacagttcat
    19501 attcagacag ctactacctt tcagtagttg taagcgacaa agctgttgat agtacatcat
    19561 taatacctgt tgtttctttt gtttattcgc aaaaaggcag aagcatccag tttcaaaatt
    19621 cttcttcagg cattgacgaa aatgctactt ttaaatggac atttggagat accaccagct
    19681 ctaatgctga aaacccaact catatatata atgccgatgg cttttacagc gttgtattga
    19741 aagtgaccca aaactataaa agctatagtt acgccaaaac aatctttatt tcagaggccg
    19801 aaatccctgc attcgacaca ttaaaaccaa ctgcatcatt ctcatattac tatcaaaaca
    19861 atgtagtgta ttttgtcaat accagttcaa acgtaaccag caatacaaaa tacacttgga
    19921 attatggaga tggcaaacct acagaaaatg ccgaaaaccc aaaccatact tttgtaaatg
    19981 gcaaatataa tgtagtgttg aaagttacaa acagcaaata tacagttacc tcaacacaaa
    20041 caataaatgt accagcatta caatcagatt atgactggag taaaaaacct gttgccaggt
    20101 ttgtttacac acgcgaaggt aaaggggttt atttccagaa tgcaagttca aacatcaccc
    20161 cggatactca gtatttatgg acttttggga ctattgaaaa cgggctccct gccacttcaa
    20221 cattagaaaa tccatatttt gtatattcaa aaccaggcac ttactcagtt gtactaaaag
    20281 taaccaatgc caatgttagc gattcatacc caatagaaat tgttatacct taatgaacat
    20341 aaaaacaaat aaaaactggt ggccctcttt tctgacattc tgggggttcg ccagtttttt
    20401 ttatgggcta ttcgtagttt ctctcaatta tacacacttc cctttcgaag ggataaaagg
    20461 atttgcaaca ataattgcac attgggcatt gattacagca agctcaggta ttattatata
    20521 ttgtttattt ttaaaccgtt gggcttttgc tctgcttttc ccaattttta caacaattac
    20581 aggtattata gcctacttta tttttcaata tgacattagc ataaacacag cagtaataca
    20641 atccactttc aacactaacc ttgacgaagc ttcaggccag atatctttgc agttgatatt
    20701 ataccagttc tttataatta caatttctgg gttcattgca ttcagaaggt ttaaagtcaa
    20761 actgcccaaa ccttattggg ttcattgggt aataattata ttgggattgt tggcaattaa
    20821 atgtattaac aattacaggt tcaataccat atatcaaaga aatccctttt cgctttatct
    20881 tgggctcaaa aattaccagc gagacaaact tgaattgtca aaaacacgga ttgacattag
    20941 caaagatgct gaatgccatg aagacagcat aacagtaatt gtggtaattg gcgaagccct
    21001 gcgtgcagat catgtcagcc tgaatggcta ttatagaagt acctttgata aggtcaaagc
    21061 attcaacccc gtttcgtttc ccaatattta ttcagagtgg acacataccc tgcaaagtgt
    21121 cccccatatt ttaacacgtg ccgatagcag caaccataag ccttcatcaa acgaaacctc
    21181 ttttatatct gttttcaggg cagttcaatt caaaacctgg tggattggca accaggacct
    21241 aagcctgata gccctgccat ttgccaaaga atgcgattct atttttatta acgagccata
    21301 taaatcagat tacagcttta ccggtaaata tgatggacag cttttgccac aaatttcaaa
    21361 agccataaat agtacagcca accgaaagct catagttgtt caacaagttg ggtgccactg
    21421 gtggtatcca tcatattatc cccctgagta cgaaatattc aagccatcta taaaaaccaa
    21481 atcatttaat gttgaagata agcaaaagat tatcaatgct tatgataatg ccatttgtta
    21541 taccgatttt tttctttctg aaatactaaa aaagctatca aaacaaaaag ctattttgtt
    21601 cttccttgcc gatcatggtg aattattggg cgaaaatggc aaatggctcc atgcacaaga
    21661 tacagaatac gagaaaaatc cggcatgctt tatttgggct tctgaaaaat accaaaaagc
    21721 atttcctgaa aaaatgaaag cattgaaaac aaaccaatac aaaaggttca ggacagattt
    21781 tcttttccat tctgtacttg atgctgctga aatctcacta cccttgtttc agaacacttt
    21841 aagcattttt gagtaaattg caattattca gatgttatac attcatctac aaaaaatgta
    21901 cattagtcaa aaaagtcagt atttgttagc ttttgcaaaa atataatatt agtttagatg
    21961 aattaaatct atctacaaat ggaattgtcc aacacttcac aaattgaact tgaagacctt
    22021 gacaaatctt ccattgacac attattgctt atcatagacg aacatcttgg acacagtttt
    22081 gatgaaagaa ttttttcggt aaaagcaaaa cagaaatttg tattgtattt atctgatagc
    22141 agtgagtgta ttcgatgtgc caaagagaaa tttattgaac ataatttcag attgtgcaat
    22201 ttgcctgata atagctatga ttcttttata attgaaatac aaaatatatg taacgaacga
    22261 acaagtaata aaatcaagct gttgatagat aatacactgg cttcttcgat ttggtatggc
    22321 tacattctca ggttttttgc caattgcgaa cttccaatgg atacgattga aatttacttt
    22381 tcgaattcat taattcaatc caatgtccca ctaaaaagtt ctaaatcaaa atttacattt
    22441 aatccattgt ttatcaattc gaataccaat atcgaaaagc ggcctttagc catcattgtc
    22501 gaacaaagct ttttcaataa agaatttatc aaactatttg aaaatcaatt taaccctaaa
    22561 tatgtaattt ggtttgttga aaaacgcatc gctgacagaa ttacattaaa tgagcaaata
    22621 tcccgggaat taaaatgcaa cgtagaaatt cataattttc ttgaaaataa agtagaagaa
    22681 acagatacag ctttgagaca gttggtaata aggttaaggc tcaattataa agtatcaata
    22741 cttaccactc attcaaaaac attctctctg atagcatcat taattaatgc aagatatcca
    22801 gacgttgaca tttggcaaca agagccagaa ccagtaaaaa taagctacga aaaaactaac
    22861 tcgttgattt ataaagctgt aatgatcaac gacgaattgg aatattaaaa aaagcgggcc
    22921 tgtaagccgg gttctgtacc tcaacgaatt gagttttcta tcatttatct cgacttaaca
    22981 ttactgctaa gctctagcgg cctacccttc tcaacaatta ccctttcgga taaaaacaaa
    23041 gcgggcaact ttgcaaacac aaggtttatt gagatataca tggccttgca acccataagg
    23101 tgtacggctc ctaatgttac catcaggacc ggtaggctct taccctgcct tttcaccctt
    23161 atcctgattt aggacctgta gaatcttaca aagtaaaatt ctcagcattc tttatcggga
    23221 cggttatttt ctgttacact actataccct cacggatatc tttctgttag aaagtatggt
    23281 gccctgtgtt gcccggactt tcctccttcg ccgaaacgaa agcgatagaa cggcccactt
    23341 ttatattaat tagccaattt gataatctga taatcagcca atgaaaatct tttaagtaat
    23401 ataatttgct aatttgctaa tttgctaatt tgctaatttg ctaatttgct aa
//
LOCUS       DAOSPF010000056        23311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2215_length_23311_cov_6.265394, whole genome shotgun sequence.
ACCESSION   DAOSPF010000056 DAOSPF010000000
VERSION     DAOSPF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2215_length_23311_cov_6.265394"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PK252_10825"
     CDS             complement(<1..1041)
                     /locus_tag="PK252_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOT15243.1"
                     /translation="MSFLSTIRNTFISKAGIVLINFLLVVVSTNLWGPEGRGNIAIVI
                     ADVSIIAIINNILAGSCVAFYTPKRGFSVIMPIAYLWTLVSTAVFSLLFSFALKHGSF
                     QYIFFITLLNSLSTLHLLYFSAKQQFKLYNILSFIQPLINIIFVFALYYVLPVKTEVV
                     YFKAYILAFFIVYIVSAIISFRQNKPRLVIDYKLGREMFVYGWQTELSSLIQFVNYRV
                     VYFLILYFAGISEVGIYSTAIAIAESIWVISRSISTVLYSKVINVASNDEGIYYTKVS
                     VRYSIWFSALLYVVLLIMPISLYSFIFGKDFGAIKPVLFFICPGILAMSLNLVYGHYF
                     SAIGNTNILLNKS"
     gene            complement(1038..2168)
                     /locus_tag="PK252_10830"
     CDS             complement(1038..2168)
                     /locus_tag="PK252_10830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15244.1"
                     /translation="MKVAFVATTHPVNDDRIFYHQAKTLCKAGFEVQIIALHAIIDEH
                     VNNLSFKTIESTDIKKKANEVLAYLSGFRPDVIVCSEPTSAYFSWKYRKKCRTKSVVI
                     YDVTEWYPSKKQHLKGVFPVKVFKSLVYVVINYIGAWVSNAFIFGEYYKAVPFKVLFP
                     FKKAIELPYYSSWEYFANLPEKDKGTKFTLCYSGKLTQEKGFVNFLKAVEQYIALNGV
                     NKIRLKIIGWFPSDGDAKVFETFRLKYNELEIVRFDFLGYYKFIEELADVDLCFDLRI
                     RCIENNHALPIKLFNYLAAGKPVVYSDLKAIRQHMPIDGLGYLVDPADHRLIADIIKQ
                     YIEDSFLYKKHSDNARNMAKTCYNWDKYSDVFVNFIKEVRKK"
     gene            complement(2263..3525)
                     /locus_tag="PK252_10835"
     CDS             complement(2263..3525)
                     /locus_tag="PK252_10835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT15245.1"
                     /translation="MKVDVLLGLQWGDEGKGKVVDVLTPKYDIIARFQGGPNAGHSLE
                     FNNIKHVLHLIPSGVFHENKLNIIGNGVVLDPIVFVKEYNELIKLGVDLKKNLVISKK
                     THLILPTHRTLDAASEAAKGATKIGSTLRGIGPTYMDKTGRNGLRVGDTLLPDFKERY
                     NSLKAKHAEILKQYNFEYNIDEQEKEWLASIEVLKQFKLVDSEHIVNNAIKKNQTILA
                     EGAQGTLLDIDFGSYPYVTSSNTICAGACTGLGVAPRSIGEVFGIVKAYCTRVGSGPF
                     PTEQINAQGEELRKKGCEYGATTGRPRRCGWLDLVALKYSIAINGVTQIILTKSDVLS
                     GMAKIPVCTHYKIDGQLTDEFPYSIENGLEPVYAELDGWNEDITTCKTYDSLPVNLKK
                     YIQFIEKETGVPVSIVSVGPNREQTIVR"
     gene            complement(3649..4323)
                     /locus_tag="PK252_10840"
     CDS             complement(3649..4323)
                     /locus_tag="PK252_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15246.1"
                     /translation="MKNIFLLIIFAFQALILSGKADYPPCQAYTPQGKDTIDAIYSYS
                     NALYAGIDNYIYVDKQKINFPVLVECTMGLAMEEDTMFIVVPSKPGKTIIRLYKEGDT
                     TGMPVFEKEMEVLAFPKPYIKYDFDRLDTLKYLSLLRLKKTNKFSVALSDDLTNESKW
                     FKVKSITVGYAIGHQYMTYSFESDTIGNEVIAKLYPGLEISLVFTISGRNGIVIRMLP
                     IKVRIY"
     gene            4492..5886
                     /locus_tag="PK252_10845"
     CDS             4492..5886
                     /locus_tag="PK252_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT15247.1"
                     /translation="MDNYEELVKRIARLVKQNKELKEVNSKLLQQNETLQEENEQFKK
                     LISELHLKPTGQKKLEEEQKTIRFNVATVLFANIHGFSKIADKTNSQAYMDELDAILY
                     HFDNIVHKYHIERIKTIGDTLMCAGGIPEKNITNPVEVVLAALEMHQILADIQMRNPD
                     KQVWELKMGIHTGPVTANISGTKKNVYDIKGDTVNIATRLESTGTPGQIVISANTYEM
                     VKEFFVCEYIGKMPIKYFGDIEMYIVKGIKPELTVNEEGLIPNQNFKIRFNLIQFTDI
                     QEIILDKLEKELPPYLYYHNVKHTVDVVTEVELIGWGEGVSDEEILLLKTAALFHDAG
                     HTIAYDNHEYHGTILAREFLPKYGYTIEQIEQICDLIMATRLPPKPKNLLEKIMCDSD
                     LDYLGRSDFIPVSNTLYKELKEQNKIGTLNEWNKLQLKFISGHQYFTQTAQNLREVNK
                     QKQIERISQLIEPD"
     gene            5897..6196
                     /locus_tag="PK252_10850"
     CDS             5897..6196
                     /locus_tag="PK252_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOT15248.1"
                     /translation="MITTCVYVEVKPEFINEFIKESEANHNEAIKEPGNLRFDILQLA
                     ENPCMFMLYEAYISEEAAAAHKTTAHYFKWRENVEKMMAKPRNGIKYNILLPKTF"
     gene            6200..7369
                     /locus_tag="PK252_10855"
     CDS             6200..7369
                     /locus_tag="PK252_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT15249.1"
                     /translation="MQKFIFHPGPALKFGNGESSALANIIKPWGDKFILVTGSQSFVN
                     LPYYQQWLTNFAAIGLIYKHIIVKGEPRAAFINEQCRMLRNNDYKFVVAIGGGSVMDA
                     AKAIAAMVTVDNDIENYLEGLPQYKQHPGTTLPLIALPTTAGTGSEATKNAVISNPGI
                     NGFKSSLRHDNFIPRLAIIDPSLHVPCPANVTAASGLDALTQLIEAYTSTQANTMTDT
                     LAIEGISLCVRSLKKAALSGDNIEARSDMALAAYLSGVVLANAGLGVVHGFASSVASM
                     FDIPHGVICGTLLGESTRMNIKKAKILNNIIALSKFTNIGKVFATESNKDDNWYLDFL
                     VQQLNKLIDEFNIQKLGAYQVTSNDLQAISKLTKIKNNPVTLTQEEILEIITSRT"
     gene            7371..7814
                     /locus_tag="PK252_10860"
     CDS             7371..7814
                     /locus_tag="PK252_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15250.1"
                     /translation="MNKFIRYAFLLCISISLFSCKGEKGDKGDPGDMGNANVKSIIFD
                     VKPSNWQGDSSEYYVFFDVKEINQEIFSNGAVLLYILRNEGSSNQSFNALPYTWVYEN
                     AFEYLDYDVFLGKIKISYRMVDNGINTTTLPDETFTFKVVVLTGN"
     gene            complement(7974..8046)
                     /locus_tag="PK252_10865"
     tRNA            complement(7974..8046)
                     /locus_tag="PK252_10865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8011..8013),aa:Phe,seq:gaa)
     gene            8334..9560
                     /locus_tag="PK252_10870"
     CDS             8334..9560
                     /locus_tag="PK252_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT15251.1"
                     /translation="MEYQKLTLNNGIRVVLLQVKTPIAHAGLFINAGSRDENQDEHGI
                     AHLIEHVIFKGTEKRRSHHILSRMEDVGGEINAFTTKEETCIHATFMKEYLERALELI
                     ADIAFNSVFPEKELNKEIEVVIDEINSYKDNPSDLIFDDFDERLFPNDPLGRNILGKA
                     EKLKRYKRKDLLTFIKNNYHTDQMVISIVGNVTIKQVEKLAIRHFGHFSANLRNKKRA
                     QALIGPPFNNSQKKSTYQTHCIIGTEAYGFTHPARMSMHLLNNMLGGPGMNSRLSMSL
                     RERNGYTYNIESNYANFSDTGIFSVYFGTDKENLDKCIKLIFKEFNKLRELKLSEIQL
                     SKAKKQLIGQLAIASESNENIMLSMGKSFLVYDKFDNLEEVCRQIEYISSIDVLETAN
                     DILNPEKLSFLIYSDK"
     gene            9557..10198
                     /locus_tag="PK252_10875"
     CDS             9557..10198
                     /locus_tag="PK252_10875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOT15252.1"
                     /translation="MKLHPDLEQYIENHSSIELPILKEIYKDTYLNQLNPRMVSGHNQ
                     GLFLIMISKMIKPKSILEIGTFTGYSAICLAQGLTPDGILHTIEIDDEIASQTKLSFN
                     RSGLGDKIKLHVGSALDIIHKINTSFDLIFIDGEKREYPQYLELCLKYLNVGGYLIAD
                     NVLWNEKVIDPAMKEDKATKAVIQFNEMVHANPDLLHVLLPLRDGLMMCQKIR"
     gene            complement(10245..10988)
                     /locus_tag="PK252_10880"
     CDS             complement(10245..10988)
                     /locus_tag="PK252_10880"
                     /inference="COORDINATES: protein motif:HMM:NF028271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT1926 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOT15253.1"
                     /translation="MMKKHIITVLVALVSLPLFSQFNAGTLKVSLNLGNSPYVGMLQA
                     PSQTSSTQYLSVEPARWVSGTSNSLVNMVGMEVKFFVADNLACKFIGGGQISVTPGQN
                     EIPGVDNDVYPFDPQTAIPAFSDVKEQKQNQFLFQVGADKYFSKNNVAIYAGGELGFR
                     YGAGKSKSITETSAGSSIAEVYGYFGAIDFGAEYNTNDGLFVGVEIRPVSVAYSVSTI
                     EPIPGVSQKGDNLNVGFFVYPTVRFGINF"
     gene            complement(11033..12775)
                     /locus_tag="PK252_10885"
     CDS             complement(11033..12775)
                     /locus_tag="PK252_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15254.1"
                     /translation="MKKNLLKLSIYGVCAVFVATSCLETEELDSVKAMREAKADKLQA
                     EADAAVLKNAYDSAKYKLDLSVATAQSQYNIADYNFQQQMLKLTYSRDSQLVVDQMAI
                     NIQNSKTNLISAQVAFVNSQATLFTAKQNYLRDSVNASAKNQALVNLYNDYFAYYNGG
                     TLSSGVTINRGIFTLRGDILAEKGTLLTALNNQANDIEIKANLILGYRDNIRTDSLSI
                     VAKQKTITIYNGAKTSADYTKAIDDITSLVNSAEVDFVAKQNARVILEDDKAKAYNAD
                     TAATNTYNRNLARKNKANNDMKNLLTLVGVNTFNELVQEQARVASDLTDKSGVYNIWA
                     TQFTTATNNLAASKNTKNQAQVRKDAADAVVASVTAAGGTPTSAQLTEQTNATQALSD
                     ANTDLAAKQSTYDNVKAGYDPAKTAYDNAKIANDSIVARRARYDGFSAIYDEADGKVA
                     SLLAAKDAKKADYEAALKQYNEALTAENIAKNNYVSYKACKTTLEGISLISDASVKIS
                     QLDDKIAALQKDIATLQNDINDYNLQISRVNKADYTYEDAIKTSNAKIAMYTSLLTEY
                     ETNAAAIKAKIDAY"
     gene            complement(13213..14562)
                     /gene="tig"
                     /locus_tag="PK252_10890"
     CDS             complement(13213..14562)
                     /gene="tig"
                     /locus_tag="PK252_10890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT15255.1"
                     /translation="MNISKQNVDELNAKIKLSFGKEDYEEKVKKSLNDYRKKINMPGF
                     RPGHVPMGLVEKMYRKPVLIEEVNKLISESLYNYIKEQNLDILGDPIPSETDNKTIDF
                     DNNTEFEFTFDIALTPSFEISWTKKDKITSYTIPADDTAINKQIEYYARRFGKFVTAE
                     VAEKESSITGNLAEVDEKGNLVENGHVAEGVTIYLDLAKDDAEINAFIGAKVGDIVKF
                     DIKKAFPNNVELANLLKVEKHEVDAAPSHFQMTIGAISKFESAAVEQDLFDKVYKAGE
                     VKSEEEFRAKIKDEVENFNKMQVKYRFANDAKKLLTEKVNFSLPEEFLKRWLLSTNEG
                     KITKEQIEQEFPMFAEGLKWQIIKNKISKDNGFKVEETEIKEMAKEEIRIQFLNYGIT
                     NMDDEHLEYFIAENMKKEESVRRYVTNVIEQKVVDYVRQNATVEEKEISQDEYAKLA"
     gene            complement(14632..14713)
                     /locus_tag="PK252_10895"
     tRNA            complement(14632..14713)
                     /locus_tag="PK252_10895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14677..14679),aa:Leu,seq:tag)
     gene            14802..15725
                     /locus_tag="PK252_10900"
     CDS             14802..15725
                     /locus_tag="PK252_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15256.1"
                     /translation="MQNIIHVRNVSKSFSNHQALSNASIEIKEGIVFGLLGPNGAGKT
                     TLIRILNQIIAPDSGEIMFKEQPLSAEDIRHFGYLPEERGLYKKMKVGEQALYLARLK
                     GLSKQEANKRLKYWFEKLKIGSWWDKRIEELSKGMAQKVQFVTTIIHEPSLLIFDEPF
                     SGFDPINANMLKNEIKELKEKGTTIILSSHNMSSVEELCDDIALINRSKVILQGNVEE
                     IRHSYRKNIFELSFTGNHEEVVTLAQQHYHILKSIQNHHIHTITLQAKGSVAENDLLK
                     IVVPHVQVLSFKEVLPGMEEIFIENVKKSQE"
     gene            15744..17108
                     /locus_tag="PK252_10905"
     CDS             15744..17108
                     /locus_tag="PK252_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT15257.1"
                     /translation="MSAYNFFLVFQREFSTRVRKRSFIIMTIIGPFLFAAMILSPLLL
                     ATYEEEELKNIAVIDSSHLFINKIPETKNLKFTYIQTDKVQEKLKTFNQEGFWGVLFI
                     SHNVTYAPNSVTLYSQSQPGTSVKYHIEESLKKEMERQLMYTNKLYDLEKTIQAIKSH
                     VNIRIVQIEEGGKMKETNDTLAMIVGYASGFLIYFFIFLFGSQVMRGVIEEKTNRIVE
                     VIVSSVKPFELMMGKIAGIAAIALIQFTAWLLLTVSFITIGQKALFPEISLSETNKVV
                     AQDIMQKQQPTQTVINIQSQAIDDKTAKMKEFYTAMNNINFGVIFGCFIFFFLGGYLL
                     YAAIFAAIGSAVDNEADTQQFMLPVTIPLILAIYVMINTMNNPDSNLSFWFSLIPLTS
                     PIVMMVRIPFGVPYWQAGLSMGLLVVTFIAFTWLAARIYRTGILMYGKKVTYRELYKW
                     IKYK"
     gene            17160..17720
                     /locus_tag="PK252_10910"
     CDS             17160..17720
                     /locus_tag="PK252_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT15258.1"
                     /translation="MAVADNLESLLDPVLFEKYFKEKYRLFCLQAFKYVQNPVIAEEI
                     VQDLFVKLWEKREELVVQGSITAYINTALKNTCLNYLKHEKVVVNYQQSYKEQNDFDS
                     QQDNDEHSFELEGHILEAIEQLPPKRKEIFLLSRVDGLKYQEIANTLGLSVKTVEAQM
                     GSALKQLRILLKDYISIVMLMLYTFF"
     gene            complement(17717..18394)
                     /locus_tag="PK252_10915"
     CDS             complement(17717..18394)
                     /locus_tag="PK252_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15259.1"
                     /translation="MQKFLIILFLGLISISSLTASNPDEPKLLVEKTYGPHLLATTFV
                     NSFPGNNNQYFNNWSEGVVLLSNGERVKIKHIRYNCLQDELLWLRPADYQTSTVLKTS
                     VNEFIVFDAENKPCYFKKEKFRDWYMLDSTFVFFHVLVEGDISFYVHHKALNIDKNSR
                     ELTRKDFYYIKSGKQLKAIKLRRSSIFNCGFFDKEKMREIIRKNHLRVRKEEDMMKAI
                     DLYNAAS"
     gene            complement(18397..20772)
                     /locus_tag="PK252_10920"
     CDS             complement(18397..20772)
                     /locus_tag="PK252_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15260.1"
                     /translation="MKIGYLILAFCLVSFGPFVFAGSKSSLLTEKKVDFSSYEKVFVK
                     TDKDIYWNGEKLFYKAWVLDAQTLKNTSSSSWLYFELVNIEGKICMSWKVNAVNGVVS
                     GITYINDTIQSGVYVLRAFTNLMRNMPEKYYYSTNILVLKLSSPEITRLEVPVYPENT
                     EDNLLCQPGGGRLVAGIPNTLYYNFNNTNKSFNAQLVDNRDSVVSNFFIDSSGIGSIS
                     FVPSIDLSYHVNIDDPIIGKIKSKPMAVFADGFIGSFNRFGEKAYFRYSTNDATLKNG
                     KYNFHIFLRDKKLLDTAIVAKPADSLVFNLKGSAGIVQVVITNSKNDTVFEQLYYCAS
                     KTNKPVVEFVQAQNEPNIKVALNLLNPALTDTLNLAFTVSYQHPFSILNINHSLNRYI
                     QIQSDLGLSLQAFNADSLSIENINCLLALIKGDQYYWNIVRNKKVFSNQRYFSPENKG
                     FYLSAQLVDGFTKQPASNKIVTMSYVDSIPNVQYAVTDTMGYCHFLLNDDAENKELIL
                     QLFKNDKDDKHIYSIIVEPKGGLKTSFKKANLMLNSEQTKYIDKIRKLRLVEKIFTNQ
                     EVKNSEKQTNELQENFRLNVKNMIKYSVFPQDYTELENMADIINNILPGVFLKEKDNT
                     ETLNVFDPNLRAVMPNQASIFLNGVLVDDLDFILKLKYTDIYKIDVCQTQLFYGDLSF
                     HGFISIITNDLAIPSSFYSSTRIKVENFVAQSQCVNKFTKEEIDQMKKEGIPDFRNIL
                     YLNYSKIPFNHGISNPISIFPSNFKGMYNFSIQGFTKDGEIFENSVQFKIQ"
     gene            complement(20793..21905)
                     /locus_tag="PK252_10925"
     CDS             complement(20793..21905)
                     /locus_tag="PK252_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOT15261.1"
                     /translation="MNAIVKIKRFVCQCGMFFMLIISISSCQEAYTDDDTTNDKKVLV
                     VNGEITDKPGPYKVTLTYASDFGSDKVTYVSGATVFLHDDKGKNVPMTEKTRGVYSTD
                     SAAIQGTVGRTYYLYIKLLDKTLYESKKQKLVDRDYTDSIYAEYGTYDYTATGTDGNI
                     IKKTLEGIYVYVDIKSNSPETKCFYFSKKVVWQYTRSLNIPGQMPQPMYFRQVINADN
                     LPDAKATQLYNGMQVIKKHLLFFVPYNVDNTNGIDSLNWGPYSSQGWVSYVFISGITK
                     EAYKFYNDAAAQLSSGMQLFDPIPSQVNSNVYCKSDSTIPVLGLFKVYTQKNKYKAFM
                     WYPTLKHVIEKDVIDPGPLVNDTFISRPQPYWVFFN"
     gene            complement(21938..>23311)
                     /locus_tag="PK252_10930"
     CDS             complement(21938..>23311)
                     /locus_tag="PK252_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15262.1"
                     /translation="VNWNHYYNENLSSTGVFAYSNYSAEKDEIEVESESSSLKTGVQY
                     FSGKYNLRYTGFSSHSIDGGLQVIHYNVSRGEMSPYNDLSIIPSSKLEKEKAFENAVY
                     LNDSWNINEKISLNLGLRYSAYFTVGPKTQLQYLSNSYTEDAIIDTVFIGNNKIGKKY
                     FGLEPRVAAKYQFNNQTSVKMSYNRNYQYITMLSYTAISIPSDVWKLADDYIRPVQSD
                     QYAIGFYRNNKENTIETSVEFYYKNLSNVTEFKNNAVVEMNPYPEQELLNAKGQNYGI
                     ELMLKKNKGKWDGWISYTYSRSLRKAISTGSGNINNGKEYPSAFDKPHDLSILANYHI
                     NKRWRMSFNFFYSTGRPFTKPEKVANIENGNKIVLYSDRNKYRIPDYHRLDFSISMDE
                     SLRKSKKWKGSWTFSVLNVYANHNTYSIFYKKEAPSAKNNYNQFSLYKLYILGIPLPT
                     LTYNFIF"
BASE COUNT         7742 a         4513 c         3781 g         7275 t
ORIGIN      
        1 agatttattc aacaaaatat tagtatttcc aatagccgaa aaataatgcc catacaccaa
       61 attaagagac atagccaata ttccgggaca tataaagaaa agaacaggtt ttattgcacc
      121 aaaatctttc ccaaaaataa atgaatacag gcttataggc ataataagca atactacata
      181 caacaatgca gagaaccaaa tactataccg caccgaaact ttagtataat aaataccttc
      241 atcattactg gctacattta tcacctttga ataaagcact gtagatatac ttctacttat
      301 cacccaaata gactctgcaa ttgcaatagc agttgaatat atacctacct ccgaaatacc
      361 cgcaaaatat aaaatgagaa agtacaccac acggtaattg acaaactgta tcaacgaact
      421 tagctcagtc tgccaaccat atacaaacat ttcgcggcca agcttataat caataactaa
      481 tcttggcttg ttttgcctga aactaataat cgcacttact atatacacaa taaaaaaagc
      541 caaaatatag gccttaaaat atacaacttc agtttttaca ggtaatacat aatacaaagc
      601 aaacacaaag ataatattga taagaggttg aataaaactt aaaatgttgt aaagcttgaa
      661 ttgttgtttt gccgaaaaat acaacaaatg taatgtggac aaggaattta aaagcgtaat
      721 aaaaaatata tactgaaagc tcccatgctt cagggcaaag gaaaatagta atgaaaatac
      781 ggcagtactc accaatgtcc acaaataagc aataggcata ataaccgaaa aaccacgctt
      841 tggagtataa aacgccacac aactgccggc aagtatatta ttgataattg caataataga
      901 aacatcagca attacaatag ctatattccc cctaccctca gggccccaga gattagtaga
      961 tacaacaacc aacagaaaat ttatcagtac tattcccgct tttgaaataa atgtattacg
     1021 tatggtactt aaaaaactca cttttttctc acctctttta taaagttgac aaaaacatca
     1081 gaatatttat cccagttata acacgtcttt gccatattcc tggcattatc tgaatgtttt
     1141 ttatacaaaa agctatcttc aatgtattgt ttgataatat cagcaatcaa cctatggtct
     1201 gcaggatcaa ctaaataacc caaaccatct attggcatgt gctgccttat agctttcaaa
     1261 tcagaataca caacaggttt tccggcggcc aggtaattaa aaagcttgat aggtaaagca
     1321 tggttatttt caatacacct gattcgcaaa tcaaaacaaa gatccacatc agcaagctct
     1381 tctataaact tgtaataacc caggaagtca aacctgacta tctccagctc attatacttc
     1441 agcctgaaag tttcaaaaac ttttgcgtca ccatcagacg gaaaccatcc aatgattttc
     1501 aacctgattt tattaacccc atttaatgca atatattgct caacggcttt caaaaaatta
     1561 acaaaacctt tttcttgagt caattttcca ctataacata aggtaaactt tgtgccttta
     1621 tctttctcag gcagattggc aaaatattcc caactcgaat aataaggcaa ttcaatagcc
     1681 tttttaaatg gaaaaagtac cttaaatgga actgctttat aatattctcc aaaaataaaa
     1741 gcattggaaa cccaggcccc aatatagttt attacaacat aaaccaggct tttaaatacc
     1801 ttaacaggaa aaactccttt taaatgctgc tttttagaag ggtaccactc tgtaacatcg
     1861 taaataacaa ctgatttagt cctacatttc tttctgtatt tccaactgaa ataagctgaa
     1921 gtaggctcgg aacaaacaat aacatctggt ctaaagcctg acaaataagc caaaacctca
     1981 ttggcctttt tcttaatgtc agtactttca atagtcttaa agctcagatt attaacatgc
     2041 tcgtcaatta ttgcatgtaa agcaataatc tgaacctcaa atccagcttt gcataatgtt
     2101 tttgcctgat ggtaaaatat ccggtcatca tttacaggat gtgttgttgc aacaaaagca
     2161 actttcattg tttttaaaca ttatattaat aagtcctgct tcggctacat caaactcaaa
     2221 gcctcaagta aaacaacgta gcatcccaat aaccatattt atttacctta caatagtctg
     2281 ttccctgtta gggccaacag aaacaatgct tacaggaacc cctgtttctt tctctataaa
     2341 ctgaatgtat tttttcaggt tcactggtaa tgaatcataa gttttacaag tggttatatc
     2401 ttcattccaa ccgtctaact cagcatatac aggctctaaa ccgttttcaa tactataagg
     2461 aaactcatct gtaagctgtc catcaatctt gtaatgcgta catacaggga tcttggccat
     2521 tccagacaga acatctgatt tggtcaaaat aatctgggta actccattaa tagctattga
     2581 atatttcaat gctaccagat cgagccagcc acaacgacgt ggacggccgg ttgtagcgcc
     2641 atattcacaa ccttttttac gtaattcttc accttgggca ttaatttgct ctgtaggaaa
     2701 tggaccactc cctacccttg tgcaataggc ctttacaata ccaaaaactt caccaatact
     2761 gcgaggtgca acacccaacc ctgtacatgc accggcacaa atggtatttg aagacgtaac
     2821 ataaggatat gagccaaaat caatatcaag caatgttccc tgcgcacctt ctgcaagaat
     2881 agtctggttt ttctttatag cattattaac aatatgctcg ctgtcaacca atttgaattg
     2941 ttttaatacc tcaatgctgg caagccattc cttttcctgc tcatcaatgt tgtattcgaa
     3001 attatattgt tttagtatct cagcatgttt agctttcaaa ctattgtagc gttctttgaa
     3061 atcaggcaat aatgtatctc ccactctaag gccgtttcta ccggtcttgt ccatgtatgt
     3121 aggaccaata ccacgaaggg tagaacctat cttcgtagca cctttggcag cttcagatgc
     3181 agcatcaagc gtgcggtgtg ttggtaaaat aaggtgtgtt ttttttgaaa taacaaggtt
     3241 tttctttaag tcaacaccaa gttttatcag ctcattatac tcttttacaa atacaatagg
     3301 gtctaaaaca actccattgc caataatatt cagtttattt tcgtgaaaaa caccagacgg
     3361 gatcaggtgc aacacatgct ttatattatt aaactccagc gaatgtccgg cattggggcc
     3421 accctgaaac cttgctatga tatcatattt cggagtaagt acatctacaa ccttaccttt
     3481 tccttcatca ccccattgca atcctaagag tacatcaact ttcattatca atttaattaa
     3541 aattttgctt taaaatcatt ttttcaaaaa acgtggcaag ttaactcaat tttttctggc
     3601 agcaaaatta aaaatcagat taaaaaaaat agttataaac agccacagct aataaatcct
     3661 gacctttatt ggcagcatcc tgatgacaat cccatttcgg ccactaatag taaaaaccaa
     3721 agaaatttcc aaccctgggt acaatttcgc aattacttca ttacctattg tatcactttc
     3781 aaatgaatat gtcatatatt gatgccctat agcatagcca acagtaatac ttttcacttt
     3841 aaaccattta gattcatttg tcaaatcatc actcaaagca acactaaatt tattcgtttt
     3901 tttcaaacgc aacaaagaca aatatttcag tgtatctaac ctgtcaaagt catacttaat
     3961 ataaggtttc gggaaagcca atacttccat ttccttttca aaaacaggca ttccggtagt
     4021 atcaccttct ttatacagcc tgatgatggt cttaccgggt tttgatggca ctacaataaa
     4081 catagtatcc tcttccatgg ccagtcccat agtacattca actaaaaccg ggaaattgat
     4141 tttttgctta tctacatata tataattatc aattccggca tacaaagcat tgctgtatga
     4201 atatatagca tcaatagtat ctttcccttg cggggtatat gcctgacatg gaggataatc
     4261 agccttaccc gataaaatca aggcttggaa agcaaatatt attagtaaaa atatgttttt
     4321 caatttttag tatattctta ttcagtgtct taatcaaaaa taatacgctc catcaaataa
     4381 tacaattctt tttattcaat ataatttggt ttaatttctg aaaagaaacc cccaacctgt
     4441 aaccaaaatt tccgttaaaa tacgctatat ttacagcaaa agacttttaa agtggataat
     4501 tacgaagaat tagtcaaacg aatagcccgt ctggtaaaac aaaacaagga gttgaaggaa
     4561 gtcaactcta agcttttgca acaaaacgaa actttgcagg aagaaaacga acagtttaaa
     4621 aaacttatca gtgaattaca tttaaaacct acaggacaaa aaaaattaga agaagaacaa
     4681 aaaacaatca ggtttaatgt tgctactgta ttgtttgcca acattcatgg cttctcaaaa
     4741 atagccgaca aaaccaactc ccaggcatac atggacgaac tcgatgctat tttgtaccac
     4801 tttgataata ttgtgcacaa atatcatatc gaaaggatta aaaccattgg cgatacattg
     4861 atgtgtgccg gtggtatccc cgaaaaaaac attaccaacc ctgttgaagt tgtattggcc
     4921 gccctcgaaa tgcatcaaat ccttgctgat attcaaatgc gcaatccaga taaacaagtt
     4981 tgggaattaa aaatgggcat tcataccggc cctgttactg ctaacattag cggaaccaaa
     5041 aaaaatgttt acgatataaa aggcgacact gtcaatatag ccacccgctt agaatctaca
     5101 ggtacccctg gccagattgt catatcggcc aacacttatg aaatggtaaa agaatttttc
     5161 gtatgtgaat acattggcaa aatgcctatc aaatattttg gcgatatcga aatgtatatt
     5221 gtcaaaggaa taaaaccaga attaactgtc aatgaagaag ggcttatccc taatcaaaac
     5281 tttaaaatac ggtttaacct tattcagttt actgatattc aagaaattat cctcgacaag
     5341 ctcgaaaaag agctccctcc ttatttatat taccataacg taaaacacac tgttgacgta
     5401 gttaccgaag tagaacttat cggatggggc gaaggtgttt cagatgaaga aatcttgctt
     5461 ttaaaaactg cagcactgtt tcatgatgca ggtcatacca ttgcttatga caatcacgaa
     5521 tatcacggta ctatcctggc ccgcgaattt ttgcccaagt atggttatac catcgaacaa
     5581 attgaacaaa tttgcgacct gattatggct acaaggctcc ctcctaagcc aaagaacctg
     5641 cttgaaaaaa tcatgtgcga ctcagacctt gattatcttg gccgtagtga ttttattcca
     5701 gtttcaaata cactttacaa agaattaaaa gaacaaaaca aaattgggac tctcaatgaa
     5761 tggaataaac ttcaactcaa gtttatctca ggacatcagt attttaccca aaccgcccaa
     5821 aatttgcgtg aagtaaataa gcaaaaacag attgaacgca tttcacaatt aattgaaccc
     5881 gactaaaata agtatcatga ttacaacctg tgtatatgtt gaagtgaagc ctgaattcat
     5941 aaacgaattc attaaagagt ctgaagcaaa ccacaacgaa gcaataaaag aaccaggaaa
     6001 cttaaggttc gacatattac aacttgctga aaacccatgc atgtttatgt tatatgaagc
     6061 atatatttca gaagaagcag ctgctgctca taaaacgact gctcactatt tcaaatggcg
     6121 tgaaaacgta gagaagatga tggccaaacc tcgcaacgga ataaaataca acattctgct
     6181 acctaaaact ttttaagcct tgcaaaagtt tatttttcat cccggtcctg cgctaaaatt
     6241 cggcaatggc gaatcttctg ctttggccaa cattatcaag ccatggggcg ataagtttat
     6301 tttggtcact ggaagtcaat cttttgtaaa tctgccatat taccagcaat ggcttactaa
     6361 ttttgccgct attgggctaa tttacaaaca tatcatcgtt aaaggtgagc ctagggcagc
     6421 atttatcaat gaacaatgcc ggatgttaag aaacaacgat tataagttcg ttgtagccat
     6481 tggcggagga agtgtaatgg acgctgcaaa agccattgct gctatggtca ctgtcgataa
     6541 tgatattgaa aattatctcg aagggcttcc ccagtataaa cagcaccctg gtacaacatt
     6601 gccacttata gcccttccga ccacagctgg tacaggtagc gaagccacta aaaatgcagt
     6661 aatttcaaat ccgggcatca acggtttcaa aagttcgctt cgtcacgaca actttatccc
     6721 acgcctggca ataattgacc cgtcattgca tgtaccttgt cctgctaatg ttactgctgc
     6781 cagcgggctt gatgccctga cgcagctaat tgaggcatat acatctaccc aagccaatac
     6841 aatgaccgat acacttgcta ttgaaggcat ttctttatgt gttcgttcat taaaaaaagc
     6901 cgcactgtca ggtgataata tcgaagcccg ctctgatatg gccctggctg catatttgtc
     6961 aggagttgta cttgctaatg caggacttgg agtagtgcat ggttttgcct catccgtagc
     7021 atcaatgttt gacattcctc atggcgttat ttgcggcact ttgttgggag aatcaacccg
     7081 catgaatatc aaaaaagcca aaatactgaa taacataata gcgttgtcaa aatttaccaa
     7141 tattggcaag gtatttgcaa ctgaatcaaa caaagacgat aactggtatc tcgattttct
     7201 ggttcaacag ttgaacaaac ttattgacga atttaacatt cagaaattgg gggcttatca
     7261 ggtaacatca aatgatttgc aggctatatc gaaattgact aaaataaaaa acaaccctgt
     7321 tacgttaaca caggaggaaa tattagaaat aataacttca agaacataag atgaacaaat
     7381 ttattcgata cgcatttcta ttatgcataa gcatctcttt attttcatgc aaaggagaaa
     7441 aaggtgacaa aggcgaccct ggcgatatgg gcaatgccaa tgttaaatcc atcatttttg
     7501 atgtaaaacc atccaactgg cagggagaca gctcggaata ttatgtattc tttgatgtaa
     7561 aagaaatcaa ccaggaaatt ttcagcaatg gtgcagtttt actatacatt ctccgcaacg
     7621 aaggctcaag taaccaaagc ttcaacgcct tgccctacac ctgggtttac gaaaatgctt
     7681 ttgaatacct tgactatgat gtatttctgg gtaaaattaa gatttcttat cgcatggttg
     7741 ataatggaat aaacacaact accttgcctg acgaaacttt tacttttaaa gtagtagtac
     7801 ttaccggaaa ttaatagtaa aatctataaa aaaagcccca tagacctgtt gctctcaata
     7861 gcagaacata aaaatcgctt caaatatttt caaaaataca ctgttttgct gcgtcttttt
     7921 tagttactta aaacaacaaa agccgctttt tacgcggctt ttgcttttag ctgtggtgcc
     7981 acgtggaatt gaaccacgga cacacggatt ttcagtccgt tgctctacca actgagctat
     8041 ggcaccttgt ctgttggaat tgcggtgcaa aaataactat ttttttttac tacccaaaaa
     8101 aatcacacaa aaacagcaaa ataatttctt tcattttatt cctttaaacc accacatttt
     8161 acaacacttt gagtgcctta tatttatcta tgctacctat aatctcctta tattctccct
     8221 tacgcttcca taatcttctt ttttttccta tccttccact tatttgcctt acctcttctt
     8281 ccttcttcct tcatcttgat tattttccct atctttgccc agctttttga acaatggaat
     8341 accaaaaatt gacacttaat aatggcatca gggttgtgct tttacaagtc aaaaccccta
     8401 ttgcccatgc aggtttgttt atcaacgccg gttctcgtga cgaaaaccaa gacgaacacg
     8461 gtatcgccca tttaattgaa catgtcatat ttaaaggaac tgaaaaacgc cgttcgcacc
     8521 atatcctttc gcgaatggaa gatgtaggcg gcgaaatcaa tgcttttact accaaagaag
     8581 aaacctgcat acacgccaca tttatgaagg aataccttga acgtgcatta gaactcattg
     8641 ctgatattgc cttcaattct gtttttcccg aaaaagaact caacaaagaa atagaagtag
     8701 taattgacga aatcaattca tataaagata acccttctga ccttattttt gacgatttcg
     8761 acgaacgtct tttccccaac gaccctcttg gccgcaatat tttaggcaaa gcggagaaac
     8821 taaaaagata caaacgaaaa gacctgttga catttattaa aaataattac catacagacc
     8881 aaatggtaat aagtattgtt ggtaatgtta ctatcaaaca agttgaaaaa ttggcaatac
     8941 gtcattttgg tcatttttcg gccaacttgc gaaacaaaaa acgggcccag gcattaattg
     9001 gcccaccttt taacaattct cagaaaaaaa gtacctacca aacacattgc attatcggca
     9061 cagaagccta tggttttaca cacccggcaa gaatgagcat gcatttgctc aacaatatgc
     9121 ttggaggccc tggaatgaac tctcgtttaa gcatgtcgct ccgcgaacgc aacggatata
     9181 cctataatat tgaatcaaac tacgccaatt tcagcgatac aggcattttt tctgtctatt
     9241 ttggtactga taaagaaaat cttgataaat gtatcaaact gatttttaaa gaattcaaca
     9301 aactaagaga actaaaactt agcgaaattc aactttctaa agctaagaaa caacttattg
     9361 gccagttggc cattgcttcc gaaagcaatg aaaatattat gttgtcgatg ggcaaaagct
     9421 tccttgtata tgacaaattt gacaaccttg aagaagtatg caggcaaatt gagtacatct
     9481 cctcaataga cgtattagaa acagccaacg acatccttaa tcccgaaaag ctttcattcc
     9541 tcatttatag cgacaaatga aactacatcc cgacttagaa caatacattg aaaatcacag
     9601 tagcatagaa ctgcctattc taaaagagat ttacaaagat acgtacctca accagcttaa
     9661 cccccgtatg gtttcgggtc acaaccaggg cttattcctt attatgatta gtaaaatgat
     9721 taaaccaaag tccatattgg aaataggaac ttttaccgga tactctgcca tttgtttggc
     9781 tcaagggtta actcctgacg gtatccttca tactattgaa atagacgatg aaatagcctc
     9841 tcaaaccaag ctttctttta atagatcagg attaggagat aaaataaaac tgcatgttgg
     9901 cagtgccttg gatataattc ataaaattaa cacctcattt gaccttattt tcattgatgg
     9961 cgagaaacgc gagtatcctc aatatcttga actatgctta aaatacctca atgtaggagg
    10021 ctatctaatc gctgacaatg ttctttggaa cgaaaaagtt atagacccgg caatgaaaga
    10081 agacaaagct accaaagctg taattcaatt caacgaaatg gtgcatgcaa atccagacct
    10141 tttacatgtt ttgttaccac ttcgagatgg attaatgatg tgccaaaaaa taagataaaa
    10201 aaaaacgccc ttaaaagggc gtttttttta ggctataagg tatattaaaa attaatacca
    10261 aatcgtaccg taggataaac aaagaaacca acattcaggt tatctccttt ttggctaact
    10321 cctggaatag gctcaatggt acttacagag taagcaacgg atacaggtct gatttcaaca
    10381 cctacaaaca acccgtcatt tgtattgtat tcagcaccaa aatcaatagc tccaaaatat
    10441 ccatatactt cagcaataga agaaccagca gatgtctctg ttatagactt tgatttacca
    10501 gctccataac gaaaccctaa ttcaccacca gcatatatcg caacgttgtt tttgctgaaa
    10561 tatttatcag ctccaacctg aaacaaaaac tgattttgtt tttgctcctt aacatcacta
    10621 aaagcaggta ttgcagtttg tggatcgaac ggatatacat cattgtcaac cccgggaatt
    10681 tcgttttgtc caggagtaac actgatttgt cccccaccta taaacttaca ggcaaggttg
    10741 tcggcaacaa aaaatttcac ttccatgcct accatgttca cgagcgagtt gcttgtacca
    10801 ctcacccaac gagccggctc aacactaagg tattgagtag agcttgtctg cgaaggagcc
    10861 tgaagcatgc caacataagg cgagttgcct aagttgagcg atactttcag tgtcccggca
    10921 ttgaactgag aaaacaaagg caatgaaact aatgctacca aaacagtaat gatatgcttt
    10981 ttcatcataa taattcttat ttcaattaaa tatttaggta tgtaacctaa tattaataag
    11041 catcaatttt tgctttaata gcagctgcat tagtctcata ttctgtaaga aggcttgtat
    11101 acattgcaat cttagcatta gatgtcttaa tagcatcttc ataggtatag tctgctttat
    11161 taacgcgaga aatctgcaaa ttgtaatcgt taatatcatt ctgcaatgtg gcaatatctt
    11221 tttgcaaggc agcaattttg tcatccaatt ggctaatttt tacagaagca tcagaaatta
    11281 aacttatccc ttcaagtgta gtcttgcaag ctttgtatga tacatagtta ttttttgcaa
    11341 tattctcagc tgttaaagct tcattatatt gtttaagagc agcttcataa tcagcttttt
    11401 tggcatcttt agcagctaac aaagatgcaa ctttaccatc tgcctcatca taaatagcgc
    11461 taaagccatc gtatcttgcc ctgcgggcta caatagagtc attagcaatc tttgcattgt
    11521 cataagctgt tttggctgga tcatatcctg ccttaacatt gtcataagta ctttgttttg
    11581 cagcaagatc tgtatttgca tcactcaaag cctgggtagc attagtttgc tcagtcaatt
    11641 gagcagatgt aggagtacct ccggcagcag taacagaagc tacaacagcg tctgcagcat
    11701 ctttacgaac ttgagcctga tttttggtgt tttttgaagc tgcaaggttg tttgttgcag
    11761 tagtaaactg tgttgcccaa atattgtaaa ccccagattt gtctgtaaga tcagaagcta
    11821 cacgggcttg ttcctgaaca agttcattaa aagtattaac accaactaaa gtaagcaaat
    11881 ttttcatatc attatttgct ttattttttc ttgccaggtt cctgttatat gtgttagtgg
    11941 cagcagtatc agcattataa gctttagcct tgtcatcttc caatattacc ctggcatttt
    12001 gttttgcaac aaaatcaact tcagcagaat ttaccaagct ggtgatatca tcaattgctt
    12061 ttgtataatc tgcagaagtt tttgctccat tgtaaatagt tatagttttt tgttttgcaa
    12121 ctatagacaa gctatctgtg cggatattat ccctataacc aaggataagg ttggccttaa
    12181 tttcaatatc atttgcctga ttgttcaatg ctgtaagtaa tgtacctttt tcagcaagaa
    12241 tatctcctct caaagtaaaa atacctctgt taatagtaac accacttgaa agtgtccctc
    12301 cattgtaata agcaaaataa tcattgtaca aattaaccaa tgcctggttt ttagcagatg
    12361 cattgacaga atctcttaaa taattctgtt ttgcagtgaa caaagtagcc tgactattaa
    12421 caaaagcgac ctgggcactg atcaggttag tttttgaatt ctggatgtta atggccatct
    12481 ggtcaacaac aagttgtgaa tccctggaat aagtaagttt caacatctgc tgttgaaaat
    12541 tataatcagc aatattatac tgactttgcg ctgtagccac agacaaatct aatttgtact
    12601 ttgccgaatc ataagcattt ttcaaaacag cagcatcagc ttcagcctga agtttatcag
    12661 ctttcgcttc gcgcatagct tttaccgagt caagctcttc agtttcaagg catgatgtag
    12721 ctacaaatac agcacacaca ccataaattg atagttttag aagatttttt ttcataaatt
    12781 tttgtttttt ggtattaaga aattaagttt tagaaataat attttattaa agaaacgtta
    12841 agttaaatat tttttttgat gtaaaaaaca ccaagcctca attaaataaa gatttttaca
    12901 ataaaatatg gttagataat aatttattat caaatttaat ataggaaaat aaaacttttc
    12961 attaatataa gttactgaac aatagattaa aagaaagttt ttaaatttag aacacccatg
    13021 attctatcca agacaaaaaa aatggtactc tggttaaata ataaaccagc tatcattcat
    13081 taaaccccat ttagcatgaa agcttttcga ctattaaaca acactaaaat tattaaaacc
    13141 aatcacccct caatgcaact tgacataaaa aaagcgccac aagggcgctt cattatattt
    13201 gttactttct gtttaagcta attttgcata ttcgtcttgc gaaatttctt tttcttctac
    13261 agttgcgttt tgacgcacat aatcaaccac cttctgctca ataacattag tcacatagcg
    13321 tctaaccgac tcctcttttt tcatattttc agcaataaaa tattcgaggt gttcatcgtc
    13381 catgttggtg atgccatagt tgagaaactg aatacggatt tcttctttgg ccatctcttt
    13441 gatttcggtt tcttcaactt taaacccgtt gtctttgctg attttatttt tgataatctg
    13501 ccatttcagg ccttcagcaa acataggaaa ttcttgttca atctgttctt tagtaatttt
    13561 accttcatta gttgaaagta accaacgttt aagaaactct tcaggtaagc taaagttaac
    13621 tttttcagtt aaaagttttt ttgcatcgtt agcaaagcga tattttacct gcattttgtt
    13681 gaagttttcg acttcatctt ttattttggc ccgaaattct tcttcagatt ttacttcgcc
    13741 ggctttgtaa actttgtcaa acaaatcctg ttcaacagca gcagattcaa acttgctgat
    13801 agcgccaata gtcatctgga aatgtgatgg ggcagcatca acctcgtgtt tttcaacttt
    13861 caacaggttt gcaagctcaa cattattagg aaaagccttt ttaatgtcaa atttaacaat
    13921 atcacccact ttagctccaa tgaatgcatt tatttctgcg tcgtctttag ctaaatcaag
    13981 ataaatagta accccttcag caacatgtcc attctcaacc aaattgcctt tttcatcaac
    14041 ttcagctaaa ttaccagtaa tgctcgattc tttttcagct acttctgcag taacaaattt
    14101 tccgaaacgg cgggcataat actctatttg tttattgatg gctgtatcat cagcagggat
    14161 agtatatgaa gtgattttgt ctttctttgt ccacgaaatt tcaaaagaag gagtcaatgc
    14221 aatatcaaaa gtaaattcaa attcggtatt gttatcaaaa tcaatggttt tattgtctgt
    14281 ttcagaagga atagggtctc ctaaaatatc aagattttgt tctttgatat aattgtaaag
    14341 tgattcgctg ataagtttgt ttacttcttc aataagtaca ggcttgcggt acattttttc
    14401 gaccaaaccc attggtacat ggccgggacg aaaacctggc atgttaattt ttttgcgata
    14461 atcattcagc gattttttta ctttttcttc gtaatcttcc ttgccaaaac taagttttat
    14521 cttggcattc aactcgtcaa cgttttgctt actaatattc attcaataaa atatttgatt
    14581 agactggata aaaagaaatt cctttttcga tggacaatta aaacaaataa gtgcggatga
    14641 agggactcga acccccacgc cttacggcac cagatcctaa gtctggctcg gctaccaatt
    14701 acgacacatc cgcaattttt gagagcgcaa agatagatat ttaatttttt ttaaagatta
    14761 atttcgcaat tatttttata ctttcactca caaaaaaaat aatgcagaac atcattcatg
    14821 tccgtaatgt aagcaaatca ttttcaaatc atcaggcatt gagtaatgct tcaatagaaa
    14881 tcaaagaagg tattgtcttt ggcctgttag ggccaaatgg agccggaaaa actactttaa
    14941 tcaggatttt gaaccaaatt atcgctccgg attctggcga aataatgttt aaagaacagc
    15001 ctttgtctgc tgaagatata cgacactttg gttacctgcc cgaagagcgg ggcttatata
    15061 aaaaaatgaa agtgggagaa caagctctat atttagccag actaaagggt ttatcaaagc
    15121 aggaagccaa taaaaggttg aagtattggt ttgagaagct taaaatcggt tcatggtggg
    15181 ataaacgcat tgaagaacta tcgaaaggga tggcgcaaaa ggtacagttt gttaccacta
    15241 ttatacatga gccttcattg ctgatatttg acgaaccgtt tagtggcttt gacccaatta
    15301 atgccaatat gctgaaaaac gaaattaaag aactcaaaga aaaaggtact actataatat
    15361 tgtcctctca caacatgtcg tcagtagaag agctgtgtga tgatattgcg ctaatcaacc
    15421 gttctaaggt cattttgcaa ggcaatgtag aggaaatcag gcacagttac cgaaaaaaca
    15481 tcttcgagct tagctttacg ggcaatcatg aagaagttgt aacccttgca caacaacatt
    15541 accatatttt aaaaagtatc caaaatcatc atattcacac tattacattg caagccaagg
    15601 gttctgttgc cgaaaatgat ttgttaaaaa ttgtggtgcc acatgttcag gtactctcat
    15661 ttaaggaggt tcttccgggt atggaagaga tttttataga aaatgtaaag aaatcacagg
    15721 aataaactta tcaatatatt gctatgtctg catataattt ctttttagta tttcaacgcg
    15781 agttttccac ccgtgtgcgt aagcgttcat ttattataat gaccatcatt ggaccgtttt
    15841 tgtttgcggc catgatactt tcaccattat tattggccac ctacgaagag gaggagctga
    15901 aaaatatagc tgtaatagac agttcacacc tttttataaa taaaatccct gaaaccaaaa
    15961 acctaaaatt tacttatata caaactgaca aagttcagga gaaacttaaa acattcaacc
    16021 aggaaggttt ttggggagta ttgtttattt ctcacaatgt tacttatgcc cccaactcgg
    16081 ttacattata ttcgcaaagc caaccgggta cttcagtaaa atatcatatt gaagaatcct
    16141 taaaaaaaga gatggaacgc caactaatgt acaccaacaa gttgtacgac ctggaaaaaa
    16201 ccatacaagc cattaaatcg catgtaaaca tccgaattgt gcagatagaa gaaggtggca
    16261 aaatgaaaga aacaaacgac actttagcca tgattgttgg ctatgccagc ggctttctta
    16321 tttacttttt tattttcctc tttggtagcc aggtaatgcg tggagtgatt gaagaaaaga
    16381 caaacaggat tgttgaggtc attgtatcat cagtaaaacc gtttgaacta atgatgggaa
    16441 agattgccgg cattgcagct atcgccctta ttcaatttac agcctggtta ttactgacgg
    16501 tttcgtttat taccataggg caaaaagctt tgtttcctga aatatcatta agtgaaacca
    16561 ataaagttgt tgcacaggac atcatgcaaa agcagcagcc aactcaaacg gtgataaaca
    16621 tccaatctca agccattgat gataaaactg ccaagatgaa agaattttat accgccatga
    16681 acaacattaa ctttggggtg atatttgggt gttttatctt tttcttcctt ggtggatacc
    16741 tgctgtatgc cgctattttt gcagccattg gttctgccgt ggacaacgaa gctgatacac
    16801 aacagtttat gttgccggtc acaatacccc ttattttggc tatttatgtg atgataaata
    16861 ccatgaacaa cccggacagc aacttatcat tttggttttc gcttatcccg cttacctctc
    16921 caatagttat gatggtacgt atcccatttg gcgtaccata ttggcaggca ggcttatcga
    16981 tggggttact ggttgttaca ttcatagctt ttacatggct tgcagcccga atataccgta
    17041 ccgggatatt gatgtatggc aaaaaagtaa cgtaccgcga actgtacaaa tggattaaat
    17101 acaaataact ttgtgatttt agcctgaaag gcttataatt gtatcataat ccataaataa
    17161 tggcagtagc agacaatctg gaatcgttgt tagacccggt gctttttgaa aagtatttca
    17221 aagaaaaata ccgtcttttc tgccttcagg cattcaaata tgtacaaaac cctgttattg
    17281 cagaagaaat cgtccaggac ttgtttgtaa aactttggga aaagcgggaa gaactggtag
    17341 tacaaggttc aattactgct tatatcaata cagcccttaa aaacacatgt ttgaattatc
    17401 tcaaacatga aaaggttgta gtcaactacc aacaaagcta caaagaacaa aatgattttg
    17461 attcgcaaca ggataacgat gagcactcgt ttgagctgga agggcatata ttggaagcta
    17521 tagaacaact tccgccgaaa agaaaggaaa tatttcttct cagccgtgtt gacgggctaa
    17581 aataccagga aatagctaac acacttggat tatcagtaaa aacagtagaa gcccaaatgg
    17641 gaagtgcctt gaagcaattg cgcatattgt tgaaagatta tatttcgata gtaatgctga
    17701 tgctttatac atttttttaa gaagccgcat tatataaatc tatagctttc atcatatctt
    17761 cttcttttct tacacgcaaa tggttttttc ttattatctc acgcattttt tctttatcaa
    17821 aaaagccaca attaaaaatg ctggaacgtc gaagtttgat agcctttagc tgttttccgc
    17881 ttttaatgta ataaaaatct tttcttgtca gttccctgct gtttttatct atatttaaag
    17941 ctttatggtg aacataaaaa gaaatgtcgc cttcaactaa tacatgaaag aaaacaaagg
    18001 tagaatcaag catgtaccaa tcccggaatt tttctttttt gaaataacaa ggtttgtttt
    18061 cggcatcaaa tacaataaac tcatttaccg aggtttttaa tacggtagaa gtttgataat
    18121 ctgccggacg caaccaaagt aattcatctt gtaaacaatt ataacgtata tgcttaattt
    18181 ttaccctttc gccatttgag agcaaaacaa caccttctga ccagttgtta aaatattgat
    18241 tattgttacc aggaaaagaa ttaacaaatg tagtagctaa caaatgcggc ccataagttt
    18301 tctcaacaag cagtttgggt tcatcagggt tagaagctgt caggctacta atagatatta
    18361 gccccaaaaa caatatgatt aagaattttt gcatagctat tgaattttga attgaacaga
    18421 attctcaaag atttctccat ctttagtaaa accttggatg ctgaaattat acataccctt
    18481 aaagtttgaa gggaatatgc ttatcgggtt tgatatacca tgattaaagg gtatttttga
    18541 ataatttaag tataaaatat ttctaaaatc aggaatacct tcttttttca tttggtctat
    18601 ttcttctttc gtaaacttat tgacacattg gctttgagct acaaagtttt caaccttaat
    18661 acgtgtactg gagtaaaatg aagaagggat agctaaatca tttgtaataa tagatataaa
    18721 cccgtgaaaa gacaagtctc cataaaacaa ttgggtttga cagacatcaa ttttgtatat
    18781 atcggtatat ttaagtttca atataaaatc taaatcatca accagcaccc cgttaaggaa
    18841 tattgaagcc tggtttggca ttacagctct taaatttgga tcaaagacat tcaaagtttc
    18901 ggtattatct ttttctttca gaaatacccc cgggagaata ttattaataa tatcggccat
    18961 gttttccaac tcagtataat cctgaggaaa aacagagtat ttaatcatat ttttcacgtt
    19021 caacctaaag ttctcctgta gttcattagt ttgcttttcg ctatttttca cttcctgatt
    19081 agtaaagatt ttttcaacca acctcaattt tcttatttta tcaatatatt tagtttgctc
    19141 agaattaagc ataaggttag cctttttaaa agaagttttc agcccccctt taggttcaac
    19201 aataattgaa tagatatgct tatcatcttt atcgttttta aacaattgca aaatcaactc
    19261 tttgttttcg gcatcatcat tgagtaaaaa atggcaatat cccattgtat cggttacagc
    19321 atactgaaca ttaggaatag aatcaacgta agacatagta acaatcttat tactggcagg
    19381 ctgctttgta aaaccatcta ctaattgggc tgaaagataa aaacctttat tttcgggaga
    19441 aaaatatctt tgattagaaa aaaccttttt gtttctgaca atattccaat aatattggtc
    19501 tccttttatt aaagccaaca aacaattgat attttcaata cttaaagaat cagcattaaa
    19561 agcttgaaga gataacccca aatcactttg aatttgaata tacctgttta aagaatgatt
    19621 gatattcaag atactaaaag ggtgttgata agaaaccgta aaggccagat ttaaggtatc
    19681 cgtaagagct gggtttaaaa ggtttaaagc aaccttaata ttgggctcat tttgagcctg
    19741 aacaaactca actacaggtt tatttgtttt tgaagcacaa taatatagtt gttcaaaaac
    19801 agtatcattt ttgctgttgg taattacaac ctgaacaatc ccggcgctgc ccttcaaatt
    19861 aaacaccaaa gaatctgctg gttttgcaac aatggcagta tcgagtaatt tcttatccct
    19921 gagaaaaata tgaaaattat atttgccatt cttcaaagtt gcatcatttg tagaataacg
    19981 gaaataagcc ttttctccaa aacgattaaa agagccaata aaaccatctg caaaaacagc
    20041 cataggcttg cttttgattt tcccaattat ggggtcatca atatttacat gatagctcaa
    20101 atcaatagat gggacaaatg aaatagagcc tataccggaa ctgtcaataa aaaaattact
    20161 aacgacagaa tctctgttat cgaccaactg ggcattgaat gacttgttgg tgttattaaa
    20221 gttgtaatac agagtattag gtatccctgc aaccaaacgc cctcctccag gttgacacaa
    20281 caaattatcc tcagtatttt caggataaac aggaacttct aacctggtaa tttcaggact
    20341 gctcaacttt aatacaagga tatttgttga ataataatat ttttctggca tattacgcat
    20401 taaattagta aaagccctta atacataaac acccgattga atggtatcat taatataagt
    20461 tattccggag actacgccat taacagcatt cactttccag ctcatacaaa tcttcccttc
    20521 aatattaacc aattcaaaat atagccaact actggaagat gtatttttta gggtttgagc
    20581 atccaaaacc caggctttat aaaataattt ttctccgttc cagtaaatat ctttgtcagt
    20641 tttaacaaaa actttttcat aggaagaaaa atcaactttt ttttcagtca acaaacttga
    20701 ttttgaacct gcaaaaacaa aaggcccaaa agaaacaagg caaaatgcca atatcaaata
    20761 tccaattttc acaactaaaa gatttaagtc cgtcaattaa aaaataccca ataaggttga
    20821 ggtcttgaga taaatgtatc attcaccaaa ggccctggat ctattacatc cttctctatg
    20881 acatgtttta atgttggata ccacataaaa gctttatatt tatttttttg ggtgtaaacc
    20941 ttaaacagtc caagcacagg tatagtagaa tcagatttac aatatacatt gctatttacc
    21001 tggctcggga tgggatcaaa cagttgcatc ccagatgaaa gctgagccgc tgcgtcattg
    21061 taaaacttat aagcttcttt ggttattccc gaaataaaaa cataagatac ccatccctga
    21121 cttgaataag gcccccagtt taaggagtca ataccattag tattatcaac gttataaggg
    21181 acaaaaaaca gcaaatgttt tttaatgacc tgcattccat tatacaactg agtagcttta
    21241 gcatcgggca ggttgtctgc attgattacc tgtctgaaat acataggttg aggcatctgt
    21301 cccggaatat ttagagatct ggtatattgc caaacaactt tcttgctaaa ataaaagcac
    21361 ttggtttcag ggctgttaga ttttatatca acataaacat atataccctc taaagttttt
    21421 tttatgatat ttccatctgt gcctgttgcc gtgtaatcgt atgttccgta ttctgcataa
    21481 atggaatccg tataatctct atctaccaat ttttgctttt tagattcata caaagtttta
    21541 tcaagtaatt ttatatataa ataataggtt cggccaacag taccctgtat tgcagcagaa
    21601 tcagttgaat acacccctct tgttttttca gtcataggga cattcttgcc tttatcatca
    21661 tgcaaaaaaa cagtagcccc actgacataa gtaaccttat ctgagccaaa atcagaagca
    21721 taggttagtg taaccttgta aggcccaggc ttgtcagtaa tttcaccatt tacaaccaat
    21781 actttcttat catttgttgt atcatcatca gtataagctt cctggcatga cgaaattgaa
    21841 attatcagca taaaaaacat accacattga catacaaacc tttttatttt tactatagcg
    21901 ttcattacta attatttgaa atacgacaat gttgttatta aaaaataaag ttataagtca
    21961 gggttggcaa aggaatacct aaaatataga gcttatataa tgaaaactgg ttataattgt
    22021 ttttagccga aggagcttct tttttataaa aaattgaata tgtgttatgg tttgcataca
    22081 cgttcaagac tgagaatgtc cagctcccct tccatttttt gctttttctg aggctttcgt
    22141 ccatactaat agaaaaatca aggcgatggt aatccgggat acggtattta tttctgtccg
    22201 aataaaggac tattttattg ccattttcaa tattagcaac tttttctggc ttggtaaatg
    22261 gcctacctgt tgaataaaag aaattgaaag acattcgcca tcttttgttg atgtgatagt
    22321 tggccaaaat ggacaaatca tgaggcttat caaatgccga aggatactct tttccattat
    22381 taatatttcc actaccagta gaaatagctt tcctcaatga tcttgaataa gtatagctaa
    22441 tccatccatc ccatttgcct ttgtttttct ttaacatcag ctctatacca taattttggc
    22501 ctttagcatt tagcagttct tgttcggggt atgggttcat ttcaacaaca gcgttatttt
    22561 taaactcggt aacattgctc aggtttttgt aataaaattc aacagatgtt tcaatggtat
    22621 tttctttgtt atttctataa aaaccaattg catactgatc ggattgcaca ggcctgatgt
    22681 aatcatcggc taacttccac acatccgaag gaatagaaat agccgtataa gacaacatag
    22741 tgatgtattg ataattcctg ttgtagctca ttttaacaga agtctggtta ttaaactggt
    22801 atttggcagc tacccttggt tcaaggccaa aatatttctt cccgattttg ttatttccta
    22861 taaaaacagt atcaataata gcatcttcag tgtagctgtt gctcaaatat tgcagttgag
    22921 ttttaggccc aactgtaaaa taagctgaat accgcaaacc aaggtttaaa gaaatttttt
    22981 cgttgatatt ccacgaatcg ttaagataaa ccgcattttc aaaagctttt tccttttcca
    23041 gttttgaaga ggggataatg gaaaggtcat tatatggaga catttctcca cgcgaaacat
    23101 tgtaatggat aacctgaagc cctccatcta ttgaatggct tgagaaaccg gtatatcgca
    23161 agttgtattt tcccgaaaag tattgaacac cagttttcag cgaagagctt tcagattcaa
    23221 cttctatttc atccttctcg gctgaataat tgctgtaagc gaacaccccg gtagaactaa
    23281 gattttcatt ataatagtgg ttccaattca c
//
LOCUS       DAOSPF010000057        23236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2221_length_23236_cov_7.862344, whole genome shotgun sequence.
ACCESSION   DAOSPF010000057 DAOSPF010000000
VERSION     DAOSPF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_23236_cov_7.862344"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1003
                     /locus_tag="PK252_10935"
     CDS             101..1003
                     /locus_tag="PK252_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT15263.1"
                     /translation="MKKRIEPTLLEKAITTIEGFDDVFKKLNQQVALRGQSPSTLNNY
                     IRCIATVSLRFGRLPELISDDEINEYLTSLALSPKSPSRSNFKHAVYGLRYYFRLIGH
                     NKRAIALPSLKKEAKLPVILNRSELRALFSAPKLLKHRILLTLAYSAGLRAQELINLK
                     LSDIDFERKTIHIRQSKYKKDRVVPLSEYMAKGLLAYLKAENPHIWLFNGKEPDGRYS
                     VRGLAWVMREALKRTSVTKAVNLHSLRHAYATHLLEDGLNIVTLKNLLGHAEITTTMI
                     YLHVAQCPLIKPHSPLDSLYPKTR"
     gene            1000..2145
                     /locus_tag="PK252_10940"
     CDS             1000..2145
                     /locus_tag="PK252_10940"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOT15264.1"
                     /translation="MSRSCVELANIATRFGKQFVEQYNPNAYHQRVLQDITQCRTAEL
                     GGHSMACDDCGQITISYNSCRNRHCPKCLASKQALWVDDLLETTLDVKHYHIVFTVPH
                     ELNPISQLNSAWFYNQMFATVWDTLRSFGYSHFGVETGAICVLHTWGQNLSLHPHVHC
                     IVPAAGESLSGRMKHIGNGGKFLYPVRQLSVTFRGKLMDAIKKQLKAIGLLPKFQQLL
                     NTAWGKQWVVFCEPSMAKPGHVVKYLGQYTHRVAITNDRILSIDGQNVCFMHKDYADH
                     ARQKPITLNGVEFLRRFCLHILPRRFVKIRRYGIYSSRYRALQKKKNPKMLVKPKETA
                     RERLARITGYDMYRCPVCKSGTLHPFEALPRIRSPDTLYAVLNTSKN"
     gene            2445..3038
                     /locus_tag="PK252_10945"
     CDS             2445..3038
                     /locus_tag="PK252_10945"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15265.1"
                     /translation="MGNSSLNPEKMHSLEFISYYMFNNKMTLGLSVYKNLIDNKLIKD
                     TIANSDRWINKNELNTYGIEVYSNCSINMFQLYTNYTYSNSYEQDGIFIPEISMHTAN
                     IGVTYSLNSNMKINLRTNYLGQRKNPKLIPSTGNDIIDDVFLLHGSVSYFDYKNFDFQ
                     LKINNILNQKYYHPSNRFAGRYRQPQRTITVKVSFNF"
     gene            complement(3133..3885)
                     /locus_tag="PK252_10950"
     CDS             complement(3133..3885)
                     /locus_tag="PK252_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOT15266.1"
                     /translation="MVILLYNTAFSQSVLHDAVKIAIYTDDPRKEIMNNIENGRTDLL
                     LIQAGQLHGHYCPGLAMGVMAAYYAIKEMKADSISMEGLRAIIETRNCVADGVQFVTG
                     CTVGNGSLIYKDTGKTSLTLVNKDGKGIRICSKQESPDIINATFPAFQEYYQKVVVKK
                     SKDSILIAKYKQASLDRNFGTLNIPFNKLFTIQHIKIKIPVNTGKNESVVCSVCGESV
                     KKTKATEVNGKIVCATCSNAAVGTFDENGIHF"
     gene            complement(4169..5281)
                     /gene="tsaA"
                     /locus_tag="PK252_10955"
     CDS             complement(4169..5281)
                     /gene="tsaA"
                     /locus_tag="PK252_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOT15267.1"
                     /translation="MYTIEPIGTVKNDFNQIMSPNIIKEKTSTIIIDDKYSEGLLNIE
                     SCEYIDVIFYFHKSEIISLKGKIHNGEERGVFASRSPKRPNSIGVTTVKLIDIIGNNL
                     LVEGLDAINGTPVIDIKCCDTSMFATGSETNLVHQSILKANPRIEIWNNILAGKTDLL
                     LLNAGQLHGHFCPGLAMGVMAATHVMKQMKTNSDGMEDLLAIVETNNCFSDGVQLVTG
                     CSFGNNSLIYRDYGKTAFTLTKRNGKGIRVVSLPESKDYINQSFPEFNECYNNIVVKQ
                     NHDAKGVAHFKKLGIERAFATLKLDFDKLFNVTEVFTEIPDYAPIYESIKCNKCGESV
                     MATRIVEKHGQQLCITCANSKYYSVEGKGIVLKKQQ"
     gene            complement(5300..6064)
                     /locus_tag="PK252_10960"
     CDS             complement(5300..6064)
                     /locus_tag="PK252_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT15268.1"
                     /translation="MMKIQIENIEFSYNGTPVLKNVTENIAKGDFVAIVGPNGSGKST
                     LLKCVNGILSTKKGTVLIDNKNLESYSSNELAKEIAYVPQSENKANPLNVFDTVLLGR
                     KPYINWKPSDSDLEITANILKKLHLEHIAMKNINKLSGGQRQCVFIARALAQQPDVLL
                     LDEPTANLDIRHQIEVLNLLKALSDNGHTIIMAVHDINLASRYCSKIMMLKDGAIFAS
                     GAKDIFTTENIEKLYDIKISIVHENGHLVIVPQGLN"
     gene            complement(6073..7137)
                     /locus_tag="PK252_10965"
     CDS             complement(6073..7137)
                     /locus_tag="PK252_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT15269.1"
                     /translation="MSIVAQYKSFTKRRILFILIVIVLLLVVSLFAIISGVAGISLHN
                     VFSVAFGQGSDFAQQVVLKIRLPRILGAILAGTVLSVSGAVMQSILRNPLASPFTLGI
                     SGAAAFGAAFAIVVFGAGSNYSGSNDAVIVHNPYIVIASAFAWSIASLAVILLISKFR
                     GATPEVMILSGIIISSLFSAGISALQYFANDVQLASVIFWSFGDLGRAGWNDVIILSI
                     VLVPLLAFFFSQSWNYKSLQSGDELAKSLGVNVSKVRLTGMVAASLCTAVVVSFYGII
                     AFVGLVVPHIVRRCIGGDEQFLIPASAVFGGTFLLFSDTIARTIISPVILPVGILTSF
                     IGAPLFLFLLIKGLGKGYWS"
     gene            complement(7134..8234)
                     /locus_tag="PK252_10970"
     CDS             complement(7134..8234)
                     /locus_tag="PK252_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOT15270.1"
                     /translation="MANLRNIKVLLLFAITLSVISCTNPSRQSTDGLVVVTDMLGREV
                     NVPKNINKVVCLRAGALRLLVYLDAVKYTTGIEELETRTNRPYILAHPELTKLPVIGP
                     SMGGDAELIAASRPDVIFITYTTVGDANALQNKTGIPVVALECPDFGVKRDTLFASLK
                     LMAKVLHKESRADSLILFINQSIGELNKRSSVIPANKKPSVYVGGIAYSGAHGIVSTQ
                     PFYPPFVFANANNIASTIDKKLVSHVKGTYIDKEQLMVWNPDIIFIDLSGLKLVREDL
                     AKNTALYNSLSAVKYDKMFGVLPYNNYAINYEVVLANAWYVAKVLYPAQFTDINIEQK
                     ADEILTMFLGKGIYKELTENTGGYRTLTKDSL"
     gene            complement(8239..10260)
                     /locus_tag="PK252_10975"
     CDS             complement(8239..10260)
                     /locus_tag="PK252_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15271.1"
                     /translation="MKSKVLALACILTLGIFPAFAQEMLKDTVTMKEVVVTGTRTARN
                     LKEIPSRVTVVNTDIMESSPVLMVDDALRFVPGINVSRSSGIFSQRPMVTLRGLSGDE
                     QSRTLVLMNGVPINTSDEGGVNWNRINPYDIDHIEIAKGPGSALYGNNAMGGVINIIT
                     KKPQKPQEIYGGISYGTFNTKRQDLSVRVRTEKGYYGSVSQYFLKSDGFNSVPDSDRT
                     AYDIKSSLEEIGISAKAGFDQSKWFKWELQYDVFRDKRGEGYQIYAPEGCYRNFNTDL
                     VRGLLKGGDEKTHYDFSAYYQMEHYYDINENMKKSGYTRYDVNSFRKEQGAILNFTRE
                     LTKDNTLTAGTEYKLGSIEGGDYYQTSPYDTVYNEGKIQTIGGYLQDEHALLDNKIKI
                     IAGLRFDQVSFMDGKYTSTSPWKTIPELKDHTWTELSPRLGLRFNFIPEVSTYLSYTH
                     GFRASILDDLTRTGWMWVGPKYANPELGPESMNNYELGIDFFPVEKLKITTSAYYSLG
                     KDFLYYVSTGDSLYGRPIFRRENVTNVTVKGAEIEASYEIGNNLNVLAGYNYCDSKIG
                     EFTKRPDLEKKSLKYVPKHSVSASLFWKNKIVNVTLRGMYKGEQYSNDANTSKIDAYT
                     TFDLQLSKTLYENFIVSLDVQDLFDNEHLETSTSLSPGRLITGRVAFKF"
     gene            complement(10348..11802)
                     /locus_tag="PK252_10980"
     CDS             complement(10348..11802)
                     /locus_tag="PK252_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOT15272.1"
                     /translation="MKKILVVVVLLPFLARIYASPMHSHYKHNIVIDTDCAIDDMRAI
                     AMLLSLPNVTVKAIIVSDGTLSPQDGVVKVRSLLAGFRKDSIPVFAGVEDKNINPAWR
                     NFNRQLQWGNTQKADVANSNLDLVADIVEQSTGQVSFICLGPLTNTANILRNHKNIIS
                     NIEEIIWYTESATPFKGFNYACDTLAVGKILDLNIKTYVISNLDKPLAVFDTTMYGIC
                     KRNTTILAKALQFVHSQPEAFKKLKENHFRLWDELAVLFLANRELFSVEPVAGKNHLR
                     YTTGYSEKAVREVMGEIIKGIYKPGEYVALYGFPLQPELYYYDVRQIMDSALQRYGIA
                     EWKACVLTDEFHGHLGVYSIVGAKMGMRARDYFGIGTDLMTVVSYAGTKPPFSCMNDG
                     IQVSTGATLGQGTISIVKDTVTRPTALFTYNNQTIEITLKKAFLEQVKADIKKGVDNY
                     GLADEAYWAFVRQNALKYWLQWDRKEIFEVKVVK"
     gene            complement(11845..13680)
                     /locus_tag="PK252_10985"
     CDS             complement(11845..13680)
                     /locus_tag="PK252_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15273.1"
                     /translation="MKKIFLFMPFSLMASQLMAQDDALNLKDTLSIDEVVVTGTPVKV
                     NRNNVPMSVSVVQREQLAESGESAVLPVLNGRVPGLFVTERGITGFGVSTGAAGQITM
                     RGIGGSPTRGVLMLIDGHPQFMGIFGHPLADSYVSSDVERVEVIRGPASILYGSNAMG
                     GAINIITRKQSQEGFHGYANAQYGSFNTQKYMAATGYKKEKLSAFVSFNHDQTDGHRD
                     NSDFSINNGYVKLGYELNTHFSANTDFSIAKFKTTDPGPDTVGATKGNSLDITRGYWA
                     FDLQDNFEKLSGATRLFYNFGKHDISDGFYSTDANYGINIHQSAGLFRGNTFTLGFDY
                     MNYGGKAENTKAMNGKGIVFKDTFLYETGVYGYIQQEFFEKLTLNAGLRLQKHEVYGK
                     HWIPSGGFAFKVTPTTTWKASAAKGFRSPSIQELYIWAHNENLSPEKVTNYETGVLQS
                     LFNHKLHVELTVFYVEGDNLIVTVPLKGVQNAGEVENKGLEMSVQANPVKNLSLITTY
                     SFIDMKSPVYATPKHNFFASLRYKWNKIGVNASIQHIKDLDTDASSKTYLSSYTLVNA
                     KLTYQVWKYAEIFASAENLLDQKYETNRYYTMPGTTAFGGVNFKF"
     gene            complement(13808..14209)
                     /gene="nikR"
                     /locus_tag="PK252_10990"
     CDS             complement(13808..14209)
                     /gene="nikR"
                     /locus_tag="PK252_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOT15274.1"
                     /translation="MALVRFGVGLEEDILKALDNFVVENNLPNRSQAIRHLIEKNIAE
                     KKWQCNHIVAGAVVLLYDHHKGDVTTKANDIQHEYFDVILSAQHFHLSHDNCLEIIAV
                     KGEAKRLTELSDKLIGIKGIIHGKLVMSRAE"
     gene            14300..14971
                     /locus_tag="PK252_10995"
     CDS             14300..14971
                     /locus_tag="PK252_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOT15275.1"
                     /translation="MINVEALFKISYGLYIVSSGNKNHGNGFISNTVFQVTAEPAQFA
                     ACCNKNNYTCSIIEQYGCFTVSVLSKDASPDLFGRFGYRSGKDFDKLEGMNITFGNTG
                     APIVLNDAIAYLECKVVQKFDAGTHVLFIGELVNASVIDDSIEPMTYAYYRKTRKGTA
                     PKNAPTFVDKSKLEPPKTTGNIYRCTACGHIYDDSKESIKFDNLPADWKCPVCGADKE
                     DFIKV"
     gene            14991..15572
                     /locus_tag="PK252_11000"
     CDS             14991..15572
                     /locus_tag="PK252_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT15276.1"
                     /translation="MSTKSLKGTRTEQNLLKAFAGESQARNRYEFFASVAKKEGYEQI
                     AAIFQETANQEKEHAKRFFKFLEGGMVEITAGYPAGVIGTTKENLKAAAEGENEEWTA
                     LYPQFAEIAKEEGFNDVATAFKMIAKVEAEHERRYLKLLQNISEDKVFMKNGKVWWKC
                     INCGYVYESEKALENCPACLHPKAFMQLKEENY"
     gene            complement(16108..17325)
                     /gene="trpB"
                     /locus_tag="PK252_11005"
     CDS             complement(16108..17325)
                     /gene="trpB"
                     /locus_tag="PK252_11005"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT15277.1"
                     /translation="MSENYFKKYPNNQGFFNEYGGAFVPPVLETEMKKINDAYFAISK
                     SHNFISELRSIRKHYQGRPTPVYYCNRLSDKYGARIYLKREDLNHSGAHKLNHCMGEA
                     LLAKFMGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEHPNVVRMQILG
                     ATVVPVSHGLKTLKEAVDSAFQAYLKDPVNTIYCIGSVVGPHPFPMMVRDFQRVVGIE
                     AREQFFDMTGELPDNVVACVGGGSNAMGIFSAFLEDDECKLYGVEPGGRSLNLGDHAA
                     TMTKGTPGLIHGFKCYLLQDEKGEPSPVYSVASGLDYPGVGPEHCMLHDMKKVCYDVV
                     SDKECIDAFYELSRLEGIIPALESAHAVAYALKLAQKEPQKSILVNLSGRGDKDIDFV
                     LEKYGLPENRKDK"
     gene            17647..18078
                     /locus_tag="PK252_11010"
     CDS             17647..18078
                     /locus_tag="PK252_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15278.1"
                     /translation="MKYLKLQKHLLLIAYFVFSTVYLTAQPEKGSKQNRNFNNQKADS
                     ICYKVSGAGTSDVNGIYKPAGKERGFTVYSNGKYKLYYKGCHSKWMIEGNNGHNMYRN
                     FIDSPIAPANKWEKACGQEALPPAPTLEVIKSDEVQAKSTK"
     gene            18285..23126
                     /locus_tag="PK252_11015"
     CDS             18285..23126
                     /locus_tag="PK252_11015"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT15279.1"
                     /translation="MKKNDTKRFFLLTVGIIMTWCTCGVLKAGNTYMVIGSDVVGLDG
                     IYTQNGTDNSKPVYSNPASFNLRYKDCTNKWVITDNSSCPYYSTYTDGNVPSTEGWHS
                     GGKSQTYFTRSVIVLPENSLGYDKKFFVESLANDGSFNDTIRIALYADGNNFTGHKGD
                     DYVTAKKVTISKLPSGLTIKVIKTSDTTLIAILSGKAYSHEYVNNINNLGITFNDIAF
                     AQRNAANINGYNCNNINVLFQDRLFVSGANNFGVNDTFNLYGLYNEKPMYVSSNNYAI
                     IYRGCKDGSQWAIINFHTTRSSGCSVYATRVVSNTIPLGSGKWFDNRGNGTDEIIVTS
                     GNAVYLSGNFNETDANDGSVDSITINYAFPKGDNKFSGDDGDDFVAGGKVSVTNVPLG
                     LTASIIRLDSVNLKFKLIGNATKHTSANSVSNLKITLNSSAFAKGSAAVGNIIHDAIQ
                     VNFKVDSLLVLGATTDASANGWYVPYGTYNGRTAYSNGSRWLWYRGCTVKWKITTNKE
                     SGCAVYSTMTESMEVPSTGWYKGRVTGSGNYSEPLTIISKNSISFDKKSFTESKTEAG
                     VLDSINISYIDISKDNKFSGNNGDDLVTTGKITVSNVPAGLTASIIRMDSVTLKFKFL
                     GKATNNNFTNSISNLAITFNNTAFESGNASEITGIKDTLKVAYLVDSFYVAGAISNNN
                     VNGWYKPNGTFNGKPSYTNGNCWIWYKNCNSGAKWKINTSVNSGCPYYSTKKDDEILP
                     LLGWNIGGHGNGKSQPLSFYLPNSIYVDNNLFVESKEAAGTTNDSANIYFLAPIDGNT
                     FSGANGEDFVTTGKVSLAKVPVGLTASVVRVDSVTLKLKLLGTATNHSFLASVNDISL
                     TINNTAFAKGNTNNIAHLKTDSLKVTFLPDSFKVFGATSYPDANGWYTMKGLFNNKPY
                     YTNGIYYIGNRNCNSRTNASKWVLASSFARLNTRSCPPYSTASDSSFAPVAGWYYGGE
                     GGQSKTEPIVILYPNSVATDKKTITESATAGVIKDTMIIYYLPTEGNAFSGANGDNFV
                     GSGKALVKNLPAGLTAKIIRTSDTSLVVYYEGKALYNKLNDGISNLSIELTSSAFTKG
                     NSIVKGCKITDISVVFYSKLMVIGSNAAVKGWYVIDGTNNNHVQFKKHGGTYYIRYKG
                     CSNYTKWAINPGGCAVSKNPVDSKVPTLTNWTNGYYLLPENSLDYSKTKLKANFSGIL
                     NNDSIVVTLYRPDTAMLSGTNGDDFVAGGKLSLSNLPSGVTVKAIRTSDTTITLYFNG
                     QILPTTYSVGIAFNSSAFSKIELTDVSFATINVTIAAPKTWTIASSGADFTSVADAVN
                     SSDVEDGDMLKVSDGTYEINNLAIRKELIIKGNSPNRTTFKSTTNRMFYTPLSSYPAK
                     VKTITFEGIGFNYSTSSNGVIEIYYAKANFNNCEFTNNQRVIYVSHGRLNVNNCTFAN
                     NSSYSNGTAIYINGDNNDTTNIYNSTFYGNKASGSGGAIYSTSDVFSAMNCTFVADTT
                     YNSGGAIYRSSNSNVSITNCLFAKNNSSSIAGNAYNINYCLFDNAQTISSGANNITNS
                     ATVNVDVLTANGGSTSTCALLTGSSAINSGTNVNAPVKDQRGVEIAESTRDIGAFEYR
                     EVD"
BASE COUNT         7208 a         4739 c         4315 g         6974 t
ORIGIN      
        1 tgttgaacta aatccctacg gggatagctt cattttagaa caaatataat tgaataatag
       61 ataacgaact tagcagcatt ttaattttaa aaaatgctgt atgaaaaaac gtattgaacc
      121 aactctgttg gaaaaagcca ttaccactat tgagggtttt gatgatgtat ttaaaaaact
      181 caaccagcaa gtagcattgc gagggcaaag ccccagcacg ctgaacaatt acattcgttg
      241 tattgctact gtatcgttgc gttttgggcg tctgcccgag cttatcagcg atgacgagat
      301 caacgagtat ttaacctctt tggcattaag cccaaagtcg ccatcgcgta gtaatttcaa
      361 gcatgccgta tatggcttac ggtattactt taggttaatt ggccataaca aacgcgcaat
      421 agccttacct tcgttgaaga aagaagctaa gcttcctgtt attcttaacc gttcagaact
      481 ccgggccttg ttcagcgcac caaaacttct gaaacatcgg attttactta cgcttgccta
      541 ttcggccggt ttgcgtgcgc aagagcttat taacctgaaa ctttccgata tcgatttcga
      601 gcgaaaaact atccatatcc ggcaaagcaa gtacaagaaa gaccgggttg ttcccctgtc
      661 tgaatatatg gcaaaggggt tgctcgcata tttgaaagcc gagaacccgc acatttggct
      721 gtttaatggc aaagaacccg atggacgtta cagcgtacgc ggattagcct gggtaatgcg
      781 tgaagccctc aaacgcactt ccgtaaccaa agcggtaaac cttcattccc tgaggcatgc
      841 ttatgccacg cacctgctcg aagacgggct gaacattgtt accctgaaaa acttgttggg
      901 acatgccgaa attaccacca cgatgattta cctgcatgtg gcccaatgtc ccctcattaa
      961 gccgcatagc ccgttggatt cactataccc caaaacccgg tgagccgttc atgtgtcgaa
     1021 ctggcaaata ttgctacccg tttcgggaaa cagttcgttg agcaatacaa ccctaatgct
     1081 tatcatcaac gggtattaca ggatattacg caatgccgca ccgcagagtt gggcgggcac
     1141 tcgatggcct gcgacgattg tgggcagata accatcagtt acaacagttg ccgcaacagg
     1201 cattgtccta aatgtttggc ctccaaacag gctttgtggg tggacgattt gctcgagact
     1261 acgctcgatg tcaaacatta ccatatcgtg tttaccgtgc ctcacgagtt aaaccccatt
     1321 agccagctca atagtgcctg gttttacaat caaatgtttg caacggtatg ggataccctg
     1381 cgaagctttg ggtactcgca ttttggggta gaaaccggtg ccatttgtgt gttgcacacc
     1441 tggggccaaa acctgagcct ccaccctcat gtacattgca ttgtcccggc cgcgggcgaa
     1501 agcttaagcg gacgtatgaa gcatattggg aacggtggaa aattcttgta cccggtacgg
     1561 cagcttagtg taaccttccg cgggaaactg atggatgcca tcaaaaagca gcttaaggct
     1621 atcgggttgc tgccaaagtt tcagcaattg ctgaacaccg cttggggcaa acaatgggtg
     1681 gtgttttgcg agccttcaat ggctaaaccc gggcatgtgg taaaatattt ggggcaatac
     1741 acgcaccggg tagctattac aaacgaccgc atactaagca tcgatgggca aaacgtttgc
     1801 tttatgcata aggattatgc cgaccatgcc agacaaaaac caatcaccct aaacggtgtg
     1861 gagttcctgc gccgtttttg cctgcatatc cttccccgga ggtttgtcaa gatacggcgc
     1921 tatggaatat acagctccag gtaccgcgct ttacagaaaa agaaaaaccc aaaaatgctt
     1981 gtcaaaccca aagaaacggc ccgcgaacgg ttggcacgta taaccggata cgatatgtat
     2041 cgatgccctg tttgcaaatc cgggaccttg cacccgtttg aagcattacc ccgtatacgc
     2101 tcgcctgata ctttgtatgc agtattgaat acttcgaaaa actaatatca ggctacaaaa
     2161 gccccccatt ttcatggggg gtagggtttg tatgccccta aagggacgaa atggcatgtt
     2221 ttaacccttc ttcgccctca atacatttaa tgaatagcca aatttgaatc gaaaaacggt
     2281 agaaaatcgt cttaaacctg tgtaccctga ctcaaaattg catggtaccg aaaatattga
     2341 aaagaaaaaa aaggtgatat gtccatagat ttctgattaa cgcccgggat ttagttcaac
     2401 cggggcttca tgttgggctt tacaagccac atgaagccct atttattggc aacagtagtt
     2461 taaatccaga aaaaatgcac tccctggaat ttattagcta ttatatgttt aacaacaaaa
     2521 tgacgttggg attgtcggtg tataaaaacc tgattgataa caaattaata aaagacacta
     2581 tagccaatag cgatagatgg atcaataaaa acgaattaaa tacttatgga atagaagtat
     2641 atagcaattg ttcaatcaac atgtttcagc tttacactaa ttatacatat tccaattcat
     2701 acgaacaaga tggtatattt attcctgaaa tttcgatgca tacagccaat attggagtaa
     2761 catattctct caattcaaat atgaaaataa acctccgaac aaattatttg gggcaacgga
     2821 aaaatcccaa gcttattccc agtactggca atgatattat agatgatgtt tttcttttgc
     2881 atgggagtgt ctcgtacttt gattataaaa actttgattt tcagctaaaa attaataata
     2941 ttctcaatca aaaatattat catccatcaa accgatttgc cggaaggtac agacaacctc
     3001 aaagaacaat tacggttaaa gttagtttta atttttaaaa ggcttattaa tttgttacct
     3061 gaattttctt gtttggcaga cagcaatcgt ttctataatc tggtctttgt tactttgtgt
     3121 gtaatatttg ttttaaaagt gaattccatt ttcatcgaaa gttccaacag cagcattact
     3181 gcatgtggca caaactattt taccattgac ttcagttgcc ttcgtttttt taacactttc
     3241 accgcaaact gaacaaacaa cactttcgtt tttacctgta tttactggga ttttaatctt
     3301 aatatgttga atagtaaaaa gcttattaaa aggaatgttt aatgtcccga agtttctgtc
     3361 taatgaagct tgtttatatt tggctatcag tattgaatct ttactctttt taacaaccac
     3421 tttctggtaa tactcctgaa aagcaggaaa tgtagcattt attatatcag gcgactcttg
     3481 ttttgaacaa atccgaatac ccttaccgtc cttattcaca agagtaaggg aagtttttcc
     3541 ggtatcttta taaattaacg agccattgcc aacggtacaa cctgttacaa actgtacacc
     3601 gtctgcaaca caatttcttg tttcgataat agcacgcaag ccctccatac tgattgagtc
     3661 ggctttcatt tcctttatag catagtatgc tgccataaca cccattgcaa gccccggaca
     3721 atagtgccca tgtaattgcc cagcttgtat tagtaacaaa tcagttcttc cgttttcgat
     3781 attattcatt atttcttttc gagggtcatc tgtgtagata gctattttaa cagcatcgtg
     3841 aagtactgat tgagaaaagg ctgtattata taacagtatt accatgagag cactacattt
     3901 aataattgat atatgttttg atttcattga tttatgtgtt ttagttatat tatttaccta
     3961 agattattta cctccttctt tcaccaatct tgatgccgac tttgagttca ttattgtgat
     4021 attatctgca acacagctac cctcacaagc cattactcca ataaaatttc taaaacaccc
     4081 gccattggca aattatactt aatcatggtg aggtctttat aaatgcaaca tttgcttatt
     4141 tgtttgtagc cctttggccg catttcattc attgttgttt cttcaaaaca attccttttc
     4201 cttcaactga ataatattta ctgtttgcac aagtgataca taattgttga ccgtgttttt
     4261 caacaatcct tgttgccata acactttctc cacatttgtt acatttaata ctctcataaa
     4321 taggtgcata atctggtatc tcagtgaata cttccgtaac gttaaaaagc ttgtcgaagt
     4381 caagttttaa agtggcaaat gccctttcta tgcccaattt cttaaaatgg gctaccccct
     4441 ttgcatcgtg gttctgtttt acaactatat tgttataaca ttcgttaaac tcaggaaagc
     4501 tttgattgat gtagtccttg gattcgggca atgatacaac ccggatgcct tttccattgc
     4561 gctttgtgag ggtgaatgct gtttttccat aatcgcgata aattagcgag ttgttaccaa
     4621 aggagcatcc ggtaacaagc tgtacgccat cagaaaaaca attgtttgtt tcgacaatgg
     4681 ccaacagatc ctccatacca tctgaattgg ttttcatttg tttcattaca tgagtagcag
     4741 ccattacacc cattgccagg ccaggacaaa aatgtccgtg caactgcccg gcattcaaaa
     4801 gtaataggtc tgtttttccg gcgaggatat tattccatat ttcaatccgt gggtttgctt
     4861 taaggattga ttgatgtaca agattggttt cagatcctgt tgcaaacatg gatgtgtcgc
     4921 aacatttaat gtcaataaca ggcgttccgt ttatagcatc caaaccttct accaaaagat
     4981 tattgccgat aatatctata agtttaacag tggtaacacc aatagaatta ggacgtttag
     5041 gacttcgtga tgcaaaaacg ccccgttctt cgccattatg tattttccct tttaggctta
     5101 ttatttctga tttatgaaag tagaatataa catcaatata ttcgcatgac tctatattaa
     5161 gaaggccttc ggaatatttg tcatcgataa taatggtact tgttttttcc tttattatgt
     5221 ttggactcat gatttgatta aaatcatttt ttacagtacc tataggttca atggtgtaca
     5281 taattattgt tttgagtttc taatttaatc cttgcggaac aataaccaaa tggccatttt
     5341 catgaactat ggatattttg atatcatata gcttctcgat attttcagtg gtaaatatat
     5401 ctttagcacc actggcaaaa attgctccat cctttagcat cattattttt gagcaatatc
     5461 gtgatgccaa gtttatatcg tgaacagcca ttattattgt gtgtccattg tcagacagcg
     5521 cttttaaaag gttcagtact tctatctgat gtcgtatatc aaggttggcc gtaggttcgt
     5581 ctaacaacag cacatctggc tgttgcgcca aagcacgtgc tataaataca cattgtcgtt
     5641 gtccgccact cagtttgtta atgtttttca tggcaatatg ttcaaggtgt agttttttca
     5701 atatattggc tgtaatttcc aaatcgctgt ccgaaggttt ccagttaata taaggtttac
     5761 ggcctaacaa tacagtatcg aatacattaa gcgggtttgc tttattttca ctttgaggaa
     5821 cataagctat ttctttagcc aattcattgg aactataaga ctcaaggttc ttattatcta
     5881 tcaatacagt gcctttttta gtggataaaa tgccattcac acacttcagt aaagtacttt
     5941 tacccgaacc attcggacca acaattgcca caaaatctcc tttggcaata ttttctgtaa
     6001 cattttttag tacaggcgtt ccattatagc taaattcaat attttcaatt tgtattttca
     6061 tcattaaaac aattatgacc aataaccctt acccaagcct tttataagca aaaacaaaaa
     6121 taaaggtgcc cctataaatg aagttaagat acccacagga agtatgacag gggaaatgat
     6181 agtacgggca atggtatcgg aaaaaagtaa aaatgtacca ccaaatacag ctgatgcggg
     6241 gattagaaat tgctcatcgc caccaatgca cctgcgtaca atatgcggaa caaccaaacc
     6301 cacaaatgca ataataccat agaatgatac tacaactgcc gtgcataacg aagcagctac
     6361 catgcctgtc aatcgtactt tagagacatt tactcctaaa cttttagcaa gttcatcgcc
     6421 cgattggaga gacttatagt tccagctttg cgaaaagaaa aaagccaata agggaactaa
     6481 aacgatagag agaattataa cgtcattcca cccggcacgg cctaaatctc cgaaagacca
     6541 gaatataaca gaagccagtt ggacatcatt tgcaaaatac tgtaatgctg agattccagc
     6601 actaaataac gaactaataa taatacctga tagaatcatt acttcaggtg tagcaccacg
     6661 gaatttggat attaaaagaa taactgccaa acttgctata ctccaagcaa aagcagaggc
     6721 aataacgata tatgggttat gtacaattac ggcatcatta gagccactat aattgctgcc
     6781 tgctccaaat acaactatgg caaaggcagc cccgaaggca gctgctcctg aaatacccaa
     6841 tgtgaaaggt gaagccagcg gattacgtaa aatgctttgc attacagcac ccgaaacaga
     6901 aagaactgtt ccagcaagta ttgcgcctaa gattcgtggt aaacgaattt tcaatacaac
     6961 ctgctgtgca aaatcagaac cctgcccgaa tgccacagaa aaaacattgt gtaaactaat
     7021 acctgctacc cctgaaatta ttgcaaacag cgacacaacc aacaacagga caataacaat
     7081 taaaataaac agtatcctgc gtttggtgaa tgatttgtat tgtgcaacaa tgctcataag
     7141 ctgtctttag ttaaagttct gtaaccacca gtgttttcag taagttcttt atatattcct
     7201 tttccaagaa acatggtcaa tatttcatcg gctttctgtt caatatttat atcggtaaat
     7261 tgtgcagggt ataaaacttt agcaacatac caagcattgg ccagaacaac ttcataattg
     7321 atggcgtaat tgttgtaagg caatacccca aacattttat cgtatttaac agcactaagg
     7381 ctattgtata aagctgtatt ctttgctaaa tcttcccgga ctaatttaag acctgataag
     7441 tcaataaaga ttatgtcagg gttccaaacc ataagttgtt ctttatcaat atatgtccct
     7501 tttacatgcg aaacgagctt cttatcaatt gttgatgcaa tattattggc atttgcgaaa
     7561 acgaaaggag ggtaaaaagg ctgcgttgat acaataccgt gtgcaccact ataagcaatc
     7621 ccccctacat aaaccgaagg ttttttattg gctggaatta ctgaagaacg tttattcaat
     7681 tctccgatag attggttaat aaataaaatt aatgaatcgg cgcggctttc cttatgcagt
     7741 acctttgcca ttagtttaag tgaagcaaac agtgtatcac gtttaacccc aaaatcagga
     7801 cattccaaag caacaaccgg gatacctgtt ttgttttgca gtgcatttgc atcaccaacc
     7861 gtagtgtagg taataaaaat aacatcgggt ctgctggctg caatgagttc tgcatcgcca
     7921 cccattgatg ggccaatgac tggtaattta gttagttctg gatgtgctaa tatgtatggg
     7981 cggtttgtcc ttgtttcaag ttcctcaatg cctgttgtat atttgacggc atctaaatac
     8041 acaagcaatc gaagcgcccc ggcacgcaaa caaactactt tatttatgtt cttaggaaca
     8101 ttcacttccc tgcccaacat atcagtaaca acaactaatc catccgtgct ttgccgggat
     8161 ggattagtgc aacttataac agaaagggtt attgcaaaaa gcaataatac tttaatattt
     8221 ctcaaattag ccattgaatt agaacttaaa ggctaccctg cctgtgatta acctccctgg
     8281 cgaaagtgag gtagatgttt ccaaatgttc attatcaaaa aggtcttgaa cgtcaagtga
     8341 tactataaaa ttttcgtaaa gagtttttga caattgcaag tcaaatgtcg tatatgcatc
     8401 aattttactt gtatttgcat cgtttgagta ttgttctcct ttgtacatgc cacgaagagt
     8461 aacattgaca attttatttt tccaaaatag tgatgcagaa acgctatgct taggcacata
     8521 tttcagcgat tttttttcca aatcaggtct tttggtaaat tcaccgattt ttgaatcaca
     8581 atagttatac ccggctaaaa cgttcaggtt attgccaatt tcatagcttg cttctatttc
     8641 agcacctttt acagttacat ttgttacatt ttctctacga aatattggac ggccatacaa
     8701 gctgtcgcct gtagatacat aataaaggaa gtctttacct aatgaataat atgcagaagt
     8761 tgttattttt agtttttcaa ctggaaaaaa gtcaatacct aattcatagt tattcattga
     8821 ttcaggcccc agttctggat tggcatattt aggtccaacc cacatccagc cagtacgggt
     8881 tagatcatca agtattgaag ccctgaaacc gtgtgtataa gataaatagg tgcttacttc
     8941 gggtataaaa ttaaaacgta accccaatct tggagagagt tctgtccaag tatggtcttt
     9001 aagctcaggt attgttttcc agggtgaggt tgaagtatat ttaccatcca taaatgatac
     9061 ctggtcgaaa cgtaaacctg caattatctt aattttgtta tcgagaagag catgttcgtc
     9121 ttgcaaatat ccacctatgg tttgaatttt tccttcattg taaactgtgt cataaggtga
     9181 tgtttgataa taatcgccgc cttcaatgct gcctaatttg tattctgttc cagcggtcaa
     9241 agtgttgtct ttagttaatt cccttgtaaa gtttaatata gcaccttgtt cttttctgaa
     9301 tgagtttacg tcatagcgtg tgtatccaga ttttttcata ttttcattta tatcataata
     9361 atgttccatc tgatagtatg cagaaaaatc gtaatgtgtt ttctcatccc ctcctttaag
     9421 taagcccctc actaaatctg tattaaaatt tctataacat ccttctggag cataaatctg
     9481 gtagccttca ccacgtttat cgcggaaaac atcatattgt agttcccatt tgaaccattt
     9541 actttgatcg aaaccagctt ttgctgatat ccctatttct tctaatgatg acttaatatc
     9601 ataagctgtt cggtcagagt caggaacact attaaaacca tcacttttta aaaaatactg
     9661 cgaaacagaa ccataatacc ctttttcagt tctaacacgg acgcttaagt cttgtctttt
     9721 tgtgttgaag gttccataac taatacctcc atatatttct tgtggttttt gtggtttttt
     9781 ggtaataata ttgataacac cacccatagc attgttccca taaagcgcag aaccagggcc
     9841 tttggcaatt tcaatatgat ctatatcata agggtttatg cggttccagt taacccctcc
     9901 ctcatcagag gtattgatgg gtactccatt cattaaaacc aatgtacgag attgttcatc
     9961 gcctgaaaga cctcgtaaag taaccatagg tctctggcta aagataccgc ttgaacggct
    10021 tacgttaata cctggtacaa accgaagagc atcatcaacc atgagcacag ggcttgattc
    10081 cataatatcg gtatttacta ctgttaccct tgatggtatt tcttttaagt ttcgtgccgt
    10141 ccttgttcct gttactacaa cttccttcat tgttactgta tctttgagca tttcttgtgc
    10201 aaatgccgga aatatgccca aggttaaaat gcatgctaat gctaaaactt tacttttcat
    10261 ttttaattca aattactatt taacaattga ctaattatgt tattaatact gagtttcttt
    10321 tactaatctc tttagttatc ttgctgctta ctttaccacc tttacctcaa aaatttcctt
    10381 cctgtcccat tgaagccagt atttcagggc attttggcgg acaaaagccc aataagcctc
    10441 atcagccagg ccatagttat ctaccccttt cttaatatca gcttttacct gctcgaggaa
    10501 agcttttttt agcgttattt caatggtttg gttattatag gtaaaaagcg ctgttgggcg
    10561 tgttactgtg tcttttacaa tacttatagt cccttgtccc agcgtagccc cggtacttac
    10621 ctgtatgcca tcgttcatgc agctaaaggg cggctttgtc cctgcataac ttaccactgt
    10681 catcaggtcg gtaccaatgc caaagtagtc gcgtgccctc atgcccatct tggccccgac
    10741 aatagaatat accccgaggt gcccgtgaaa ctcatcggta aggacacatg ctttccactc
    10801 ggcaatacca tagcgctgta aggcagagtc cataatttgc cgtacatcat agtagtacaa
    10861 ttcaggctgc aacgggaagc catacaaagc aacatattcg cctggtttgt atatgccttt
    10921 tattatttct cccattactt cgcggactgc cttttcagaa taacctgttg tatagcgcaa
    10981 gtgattcttg ccagccacag gctcaacgct aaaaagctca cggttggcaa gaaacagcac
    11041 cgccagctcg tcccaaagcc tgaaatggtt ttctttgagt tttttaaaag cctcgggttg
    11101 cgaatgaaca aactgcaatg cttttgccaa tatagttgta ttccttttac aaataccgta
    11161 catcgtggta tcgaacacag ccaaaggctt atcaagatta gaaataacat aggtcttgat
    11221 gttcaaatca agaattttgc caacagccag ggtgtcgcac gcataattaa agcctttgaa
    11281 aggggttgca gactcggtgt accatattat ttcttctata ttcgaaatga tgtttttatg
    11341 attccgaaga atattggctg tattggtcag cggcccgaga caaataaatg aaacctgccc
    11401 cgtcgattgt tcaacaatat cggccaccaa gtcaaggttg ctatttgcaa catcagcttt
    11461 ttgagtattt ccccattgca actgacggtt gaaatttctc caggccgggt tgatgttttt
    11521 atcttcaacg cctgcaaaaa ctgggatact atctttgcga aatcctgcca gcaatgacct
    11581 cacttttacc actccgtctt gaggtgacag cgtcccgtct gaaacaatga tggccttaac
    11641 tgtaacgtta ggcagtgata aaagcatagc aatagcccgc atatcatcaa tggcacagtc
    11701 ggtgtcaatg acaatattat gtttgtaatg gctatgcata gggcttgcat aaatcctggc
    11761 caaaaatggc agcaatacta caacaactaa gatttttttc atttttttaa tggataatta
    11821 ttatatcaaa agaattccgg ctggtcaaaa tttgaagtta acaccgccaa aagcagtagt
    11881 accgggcata gtataatacc tgttggtctc atacttttgg tctaaaaggt tttcggcact
    11941 ggcaaatatc tcagcgtatt tccaaacctg gtatgtaagc tttgcattca ccaatgtata
    12001 gctgctaagg taggttttgg acgaagcatc ggtgtcaagg tctttaatat gttgtatgct
    12061 ggcattgaca ccaattttgt tccatttgta cctgaggctg gcaaaaaagt tatgtttagg
    12121 agttgcataa acagggcttt tcatatctat aaagctatat gttgtaatta aagaaaggtt
    12181 tttaacagga ttggcctgaa cagacatttc caaccctttg ttttctacct cgcccgcatt
    12241 ctgcacccct tttaacggaa cggtaacaat gaggttgtca ccttcaacgt aaaataccgt
    12301 aagctctaca tgaagcttat ggttaaaaag cgattgcaaa acgccggttt cgtagtttgt
    12361 aaccttttcg gggctaaggt tttcgttatg agcccaaatg taaagctctt gtatagaggg
    12421 actgcgaaag ccttttgccg cagaagcctt ccaggtagtc gttggcgtaa ctttaaatgc
    12481 aaaacctccc gaaggtatcc agtgttttcc atacacttca tgtttttgca aacgtaaacc
    12541 tgcatttaat gtaagtttct caaaaaactc ctgctgtata tacccataca ctccagtttc
    12601 gtatagaaat gtatctttaa aaactatccc tttgccattc attgctttag tattttcggc
    12661 tttgccgcca taattcatgt agtcaaaccc taaagtaaat gtgttgcccc tgaacaagcc
    12721 tgctgactgg tggatgttta taccatagtt ggcatctgta gaataaaaac catcactaat
    12781 atcatgttta ccaaaattgt aaaacaaccg tgtagcgccc gacaattttt caaaattatc
    12841 ctgtaagtca aaagcccagt aaccacgggt aatatcaagc gaattacctt tagtggcacc
    12901 aactgtatcg gggcctgggt cagtagtttt aaattttgca atactaaaat ctgtattggc
    12961 cgaaaaatga gtattgagct catatcctaa ctttacatac ccattattta tgctgaaatc
    13021 tgaattgtca cgatgtccat cggtctggtc gtggttgaac gaaacaaatg cacttaactt
    13081 ttccttttta taacctgttg ctgccatata cttttgagta ttgaacgagc catactgggc
    13141 attggcataa ccatgaaacc cttcttgcga ctgtttgcgg gtgataatgt taattgcccc
    13201 gcccatggcg ttagaaccat aaagtataga agcagggccc cggataacct ccactcgctc
    13261 tacatcagag cttacatacg aatcggccaa cgggtgccca aaaatgccca taaactgagg
    13321 atgcccgtca ataagcatca acaccccacg ggtagggctt ccaccaatac cgcgcatagt
    13381 gatttgcccc gccgctccgg tcgaaacccc aaaaccggta atgccacgtt cggtaacaaa
    13441 taaaccgggt acacggccat tcaacacagg caatactgcc gactcgccac tttcagccaa
    13501 ctgctcacgc tgtaccaccg aaacagacat aggtacatta ttgcggttta ctttaaccgg
    13561 agtcccggtt actacaactt cgtcaatact taatgtatct ttgaggttta aagcatcatc
    13621 ctgtgccatt aactgacttg ccatcaaaga aaaaggcata aataaaaata tcttcttcat
    13681 ctcttattta taatatgtta ctttttaaaa aatcgtgtta ctattgttaa aaaaaataaa
    13741 cccaaacggg tttttgttta aaatattact tttattaaaa ttcgtgttac tgcataacca
    13801 aaaaaaatta ctctgcccgg ctcatcacca acttaccatg tattatgcct ttaatgccaa
    13861 ttagtttgtc agaaagctcg gtcaggcgct tagcttcgcc cttaacagca ataatttcga
    13921 ggcaattatc atgacttaga tgaaaatgct gggccgatag tatcacatca aaatattcgt
    13981 gttgaatatc attagctttt gtagtaacat cacctttatg atgatcgtaa agcaaaacaa
    14041 ctgccccggc tacaatatgg ttacattgcc attttttttc ggcgatgttt ttctctatca
    14101 ggtgacggat ggcttgcgaa cggttaggca ggttgttttc gacaacaaaa ttatccaaag
    14161 ccttcagtat atcttcttct aaaccaacac caaacctgac aagcgccatt gtgttactat
    14221 ttaatttttt cgtggcacaa agatatattt tattttacta aaattaaaag caagttttta
    14281 ataactttaa gaaaaaaaca tgataaatgt tgaagctctt tttaaaatat cgtatggcct
    14341 gtatattgtg agctctggca ataaaaacca cggcaatggc tttatttcca acacggtatt
    14401 tcaggtaact gccgagcctg cccaatttgc ggcctgttgc aacaaaaaca actacacttg
    14461 cagcattatt gagcagtatg gatgctttac ggtttcggtg ctgagtaagg atgcctcacc
    14521 tgatcttttt ggaaggtttg gttatcggag cggcaaagat tttgacaagc tcgaaggcat
    14581 gaacatcact tttggaaaca ccggagcccc tattgtactg aacgatgcta ttgcttacct
    14641 tgaatgcaaa gtggtacaaa agtttgatgc gggcacccat gttttgttta ttggcgaact
    14701 ggtaaatgcc agcgttatcg atgatagcat tgagcccatg acatatgctt actaccgcaa
    14761 aacacgcaag ggcacggcgc ccaaaaatgc ccctacattt gtcgataaat cgaaacttga
    14821 gcctcctaaa accactggca acatttatag atgcacagct tgtggccaca tatacgatga
    14881 tagcaaagaa agtattaaat ttgacaactt gcctgccgac tggaaatgcc cggtttgtgg
    14941 tgctgacaaa gaagatttta taaaggttta accaattaat aattagcact atgtcaacaa
    15001 aatcattgaa aggtacacgt accgaacaaa acctgctaaa ggcttttgca ggtgaatcac
    15061 aagcccgcaa ccgctatgaa ttttttgcca gcgtagccaa aaaagaaggt tacgagcaaa
    15121 tagctgccat tttccaggaa actgctaacc aggaaaaaga acatgccaaa cgtttcttta
    15181 agtttctcga aggcggtatg gtcgagatta cagccggata tcctgccggc gttatcggta
    15241 ccaccaaaga aaacctcaaa gctgctgccg aaggcgaaaa cgaagaatgg actgcattat
    15301 atccacaatt tgccgagatt gccaaggaag aaggctttaa cgacgtggct acagctttca
    15361 aaatgattgc caaagtagaa gccgaacacg aacgccgcta tttgaaactt ctacagaata
    15421 tctctgaaga caaggtattt atgaaaaatg gcaaagtatg gtggaaatgc atcaactgtg
    15481 gctatgttta cgaatcagaa aaagcgctgg aaaactgccc tgcatgcttg cacccaaaag
    15541 cattcatgca attgaaagaa gaaaattatt agtttctgaa aatactataa aactaaaagg
    15601 ccgcttctat ttgaaacggc cttttttatt actatttgtc ctggattcaa tataagaaat
    15661 tcattatcaa tatattgaat agcaataaat actgagtctc tacgcaactt tttttatcga
    15721 aaaaagttgc tcaaaaaatc ttatcgcctc aactcaatca ccctcgaagc ctaagaatgt
    15781 tacaatatgc aaacaacagg agcaatacgc ttctcggccg aaatcgaaca aaaagacgat
    15841 gaaacggaat ctttctgtta aaggcaccgc cgttttcatt gaatcttttc tactaatggc
    15901 ctgacgtaaa tggcgttaag aaaaaagctt tatcggcgaa gcgagggtac tgtttgagtg
    15961 aagcgagttg ttgcccggaa ccgccgataa agctttttta gccgatttac ggcaagcctt
    16021 gaatttttgt tacttttgct tcaaggcaaa agtaaggaat gcctgtaaat atgaatatgt
    16081 aagctttttt cttatgtcgc actgacgtta tttgtccttt ctattttcag gaaggccata
    16141 tttttcgagt acaaagtcaa tatccttatc accgcgtccg ctaaggttaa ccagaattga
    16201 tttctgtggt tccttttgag ctaattttaa agcgtaagca acagcgtgag cactttccaa
    16261 cgcagggata ataccttcca aacggcttag ttcataaaag gcatcaatac attctttgtc
    16321 cgaaaccacg tcgtagcaga cttttttcat atcgtgaagc atgcaatgct ctgggccaac
    16381 gcccggatag tcgagtccgc ttgctacaga ataaacaggt gaaggttcgc ctttttcgtc
    16441 ctgcaacaaa taacatttga agccgtgaat caatcccgga gtccctttag tcatagtagc
    16501 agcatggtcg cccaggttga gcgaacggcc accaggctca accccgtaca atttacattc
    16561 atcgtcttcg aggaaagcac tgaaaatacc catagcattg ctgccgccgc caacgcaggc
    16621 aacaacgttg tcgggcaatt cgccggtcat gtcaaagaac tgttccctgg cctcgatacc
    16681 aaccacgcgt tgaaaatcgc gcaccatcat tgggaatggg tgtggcccca caaccgagcc
    16741 aatacaataa atagtattta cagggtcttt caaatatgcc tgaaatgccg agtcaactgc
    16801 ttcttttaac gtttttaacc catgagatac aggaacaacg gtagctccaa gaatctgcat
    16861 acgtactaca tttggatgtt ccttggcaat atccacttca cccatgtgta tctcgcactc
    16921 aagcccgaaa taagcggcag ctgtagcaag ggcaacgcca tgttgaccag ccccggtttc
    16981 ggcaatcagc ttcttcttcc ccataaactt tgccaacagg gcttcgccca tacagtggtt
    17041 aagtttgtgg gcaccactat ggttcaaatc ctcacgtttc aggtaaatcc tggcaccata
    17101 cttgtctgaa agccggttgc aataatacac aggcgttggc cgtccctggt aatgcttgcg
    17161 gatgctgcga agctcagata taaagttgtg cgacttgcta atagcaaaat acgcatcatt
    17221 gattttcttc atttctgttt caagcacggg aggaacaaat gcccctccat attcgttaaa
    17281 aaatccttga ttattgggat acttcttaaa ataattttct gacattttat tagtttttat
    17341 attgtaaata attagtgttc aaatactttt taattactgt tgaccgggat aaaaataatg
    17401 aaaaaaatta ccattacgtg atttcctaat gatggaaata taaatgtttt tcatttttga
    17461 tgaggcaaaa tgaataacct tgataaagtc catatattac caaactcttt gactaataga
    17521 gaaaaaaaac aaaaacagat atgaatatcc attatattgc gataaaaaaa aaatatcatc
    17581 cttttttact tttttcttgc tgcaaataaa gaaaaaaagt aatttagccc ctttaaccta
    17641 ttaatcatga aatacctaaa actgcagaag catttgctgc taattgccta ttttgttttt
    17701 tcaacagtat atttaaccgc ccaacctgaa aaaggttcaa agcaaaatag aaatttcaac
    17761 aaccagaaag ccgacagtat ttgttacaaa gtcagtggtg ccggaacatc cgatgtcaac
    17821 ggaatttata aaccagccgg aaaagaacgc ggtttcacag tttacagcaa tggcaaatac
    17881 aaactttact ataaaggctg ccattcaaaa tggatgatag aaggcaataa tgggcataac
    17941 atgtaccgca attttattga cagccctata gctccggcca ataagtggga aaaggcctgc
    18001 ggtcaggaag cattacctcc ggcccctacc cttgaggtga taaagtcaga cgaagttcag
    18061 gcaaaaagca caaaataacc atgcaaagaa gcctataata tcgacaataa accctcaaac
    18121 cccaaacaac ataataaatg aaacatacct taacagcgat aaccttaaac aagtattatc
    18181 gcataacaca aacgagtgta ggctattata agtgctaaac acttttagct atatagaaaa
    18241 aaatacaaat taaaatagac cttttttaac taaatcttac acaaatgaaa aaaaatgaca
    18301 caaaacgatt ttttctattg actgtaggga ttatcatgac ctggtgtact tgtggtgtac
    18361 ttaaggcagg taatacttat atggtcattg gatcagacgt tgtgggatta gatggtatct
    18421 atacccaaaa cggcactgac aacagcaaac ctgtttattc aaacccggca agtttcaatc
    18481 ttaggtataa ggattgtact aataagtggg ttatcaccga caactcaagc tgcccatatt
    18541 acagtacata taccgatgga aatgtaccat caactgaagg ctggcatagc ggcggtaaat
    18601 ctcaaactta tttcacccgt tcggtcattg tgttgcctga aaattcattg ggttacgaca
    18661 aaaagttctt tgtagaatcg ttggccaatg atggtagttt taacgacacc atcaggattg
    18721 ctttatatgc tgacggtaac aactttaccg gtcataaagg cgatgattat gtgaccgcca
    18781 aaaaagtaac tattagcaag ctgccttcag gattaaccat aaaagtaatt aaaacaagtg
    18841 atacaaccct tatagctatt ctttcgggga aagcatactc tcatgaatat gttaataaca
    18901 ttaataatct tggcatcacc tttaatgaca ttgcttttgc acaacgtaat gccgctaata
    18961 taaacggcta taactgcaac aatatcaatg tcttatttca ggacaggtta tttgtttccg
    19021 gagctaacaa ttttggggta aacgacactt ttaaccttta tggcttgtac aatgaaaaac
    19081 cgatgtatgt aagcagcaat aattatgcta ttatatatag agggtgtaaa gacggtagtc
    19141 aatgggcaat aataaatttc cataccacaa gaagcagtgg ttgttctgtt tatgctaccc
    19201 gtgttgtaag caatactata cctttaggtt caggaaagtg gtttgataat agaggtaatg
    19261 gaactgatga aattatagtt acttcaggca atgctgttta tttgagcggt aattttaatg
    19321 aaaccgatgc taacgacggt tccgtagata gcattacaat aaactatgcc ttcccgaaag
    19381 gagataataa attttcgggg gatgatggcg atgattttgt tgccgggggc aaggtttcag
    19441 taaccaatgt acctctgggg cttactgctt ctataatccg tcttgactca gtgaacctga
    19501 aattcaagct tattggcaat gcaactaaac acacttccgc caattcagtt tccaacttaa
    19561 aaataacact taacagttca gcttttgcaa aaggctctgc agctgttggc aatattattc
    19621 atgatgctat acaagtaaat ttcaaggtgg acagcttatt agtattagga gccactacag
    19681 atgcttctgc taacggatgg tacgttccct atggtactta taacggaaga acagcatact
    19741 caaatggttc acgctggtta tggtaccgtg gttgcactgt taaatggaaa ataacgacta
    19801 ataaagaaag tggatgtgct gtatattcaa caatgacaga aagcatggaa gttccatcta
    19861 caggatggta taaaggacgc gttacaggtt ccggaaatta ttcagaaccc cttaccatta
    19921 taagtaaaaa ctctatttcg tttgacaaaa aatcatttac agagtcaaaa actgaggctg
    19981 gagtactcga tagcattaac atatcctata tcgacatctc aaaagacaac aagttctcag
    20041 gtaataacgg agatgacctt gtcactactg gaaaaattac tgtaagtaat gtaccagcag
    20101 ggttaacagc atctattatc cgcatggatt ctgttactct gaaattcaag tttttaggga
    20161 aagctactaa taacaatttt acaaattcaa ttagtaacct tgccattact ttcaacaaca
    20221 ctgctttcga atctggtaat gcttcagaaa taaccgggat aaaagatact ttaaaggtag
    20281 cttatctggt tgacagcttc tatgtagctg gtgcaatatc taataacaat gttaatggct
    20341 ggtataagcc caatggcaca tttaacggga agccaagtta tacaaatggt aattgctgga
    20401 tatggtacaa aaactgtaac agtggagcta aatggaagat taacacatcg gtaaatagcg
    20461 gatgccctta ttattcaaca aaaaaagatg acgaaatatt gccattatta ggatggaata
    20521 ttggcggcca tggaaatgga aaaagtcaac cactaagctt ttatttaccc aattcaatat
    20581 atgttgacaa taatttattt gtagaatcaa aggaagctgc cggaacaacc aatgacagtg
    20641 ccaatattta tttcttagcc cctattgatg gcaatacatt cagtggcgct aatggcgaag
    20701 actttgttac aaccggaaaa gtttctttag caaaagtacc ggtaggatta acagcttctg
    20761 ttgtccgtgt tgactccgta acactgaaac tcaagttgtt aggcacggct accaaccact
    20821 cattccttgc ttcggttaac gacattagcc ttacaataaa caatactgct tttgcaaaag
    20881 gcaacactaa caatattgct catttaaaaa ccgactcatt gaaggtgacg tttttaccag
    20941 acagtttcaa ggtatttggc gcaacatctt atccagatgc caatggatgg tacacgatga
    21001 aaggtctatt caataacaaa ccatattaca caaatggtat ttattacatt ggaaaccgca
    21061 attgtaatag caggacaaat gcctcaaaat gggtactggc tagttcattc gcaagactta
    21121 atacaagatc ttgtcctcct tattctacag cttctgacag ctcttttgcc cctgttgcag
    21181 gatggtacta tggtggagaa ggtggacaaa gcaagacaga acctattgtt attctttacc
    21241 ctaatagcgt tgccactgac aaaaaaacaa taacagaatc agctactgcc ggagttatta
    21301 aagataccat gattatttat tatcttccaa cagaaggcaa tgcatttagt ggtgctaacg
    21361 gtgacaactt tgttggttca ggtaaagcat tagtcaaaaa cctgccagca ggcttaacgg
    21421 ctaaaattat ccgcacaagt gacacatcat tagtggtata ttatgaaggc aaagctttat
    21481 ataataaatt aaatgatggc atttccaact taagcatcga acttacctca tctgctttta
    21541 ctaaaggcaa ttcaattgtt aagggttgta aaataacaga catttctgtt gttttttata
    21601 gtaaattaat ggttatagga agtaatgcgg ctgtaaaagg ttggtatgtg attgatggaa
    21661 caaataataa ccatgtacaa tttaaaaaac atggtggcac atattacatc cgttataaag
    21721 gttgttctaa ctatactaaa tgggctatca accctggagg ttgtgcagtt agtaagaatc
    21781 ctgtagatag caaggttcca acattaacaa actggacaaa cggttattat ttattgcctg
    21841 aaaatagcct ggattattct aaaacaaagt taaaagctaa ttttagtggc atcttaaaca
    21901 acgactctat tgtagttaca ttgtatcgcc cagatacagc aatgttgtcc ggcaccaatg
    21961 gcgatgactt tgttgcaggc ggaaagcttt cgctcagcaa tttaccttcg ggtgttacag
    22021 taaaagctat acgtaccagc gacaccacta taaccttata ttttaacgga caaatactac
    22081 ctactacata ctctgttggc atagcattca atagtagtgc tttcagtaag atagagctta
    22141 cagatgtatc attcgctact attaatgtaa ctatagctgc tccaaaaaca tggactattg
    22201 cttcttcagg tgctgacttt acttcagtag ctgatgcagt aaacagcagt gatgttgaag
    22261 atggagacat gcttaaagtt tcggatggaa cttatgaaat aaataacctg gctatcagga
    22321 aagaacttat cataaaaggg aatagtccta atagaactac tttcaagtca accacaaatc
    22381 gtatgtttta taccccgtta agttcgtacc ccgcgaaggt caaaaccatt actttcgaag
    22441 gtattggctt taattattca acaagtagca atggtgttat agaaatatat tatgctaaag
    22501 caaatttcaa caattgcgaa tttactaaca accagcgggt tatttatgtt agtcatggaa
    22561 ggttgaatgt aaacaattgt acttttgcca acaattcttc atattcaaat ggaaccgcta
    22621 tctacatcaa tggcgacaac aacgacacta ccaatattta caactctaca ttctatggca
    22681 acaaggcttc aggatcagga ggggctatat attcaaccag tgatgttttc agtgcaatga
    22741 actgtacatt tgtggctgat acaacttaca attcaggtgg tgctatttat agaagcagca
    22801 actcgaatgt atctataacc aactgtttgt ttgccaaaaa taacagtagc agtattgctg
    22861 gaaatgcata caatatcaat tactgtttat ttgacaatgc ccaaacaatt tcttcaggag
    22921 caaacaatat aacaaacagt gctactgtaa atgttgatgt attgactgca aatggaggct
    22981 caaccagtac ttgtgctttg ttaacaggaa gttctgctat caacagcgga acaaacgtta
    23041 atgcccctgt taaagaccag agaggtgttg aaatagctga aagtacacgt gatattggag
    23101 cttttgaata tagagaagta gattaagaat aaattaataa aaatcatttt tatgtaatga
    23161 ttggttaagt taaaaggcta ataaatgggt ctaattataa ggagtatatc aatttcttgc
    23221 ctcattttgg ctaatt
//
LOCUS       DAOSPF010000058        22545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2302_length_22545_cov_7.774033, whole genome shotgun sequence.
ACCESSION   DAOSPF010000058 DAOSPF010000000
VERSION     DAOSPF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_22545_cov_7.774033"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK252_11020"
     CDS             <1..210
                     /locus_tag="PK252_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HOT15280.1"
                     /translation="DWQNQYNLYKGAGLGLSHSMLQIGAFRPKNYDEIFKNTFYVGAS
                     TVPGAGLPMAIISSKLTVERVYALG"
     gene            complement(316..699)
                     /locus_tag="PK252_11025"
     CDS             complement(316..699)
                     /locus_tag="PK252_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15281.1"
                     /translation="MKTLKFISVAIFMLFFASCGKYSSSEKEVIKQKIIAMYNQVPKM
                     TMQNAAGYDFNSSNAICREVVQKDSVLTGFFNYIFTDQKSETGVPLSIEYANFASIPV
                     QFLVKDALELTKEEFDSIRVARVIF"
     gene            complement(731..1492)
                     /locus_tag="PK252_11030"
     CDS             complement(731..1492)
                     /locus_tag="PK252_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15282.1"
                     /translation="MKTIAYILCLAVFVTACTKTPQTIAEKEVKSFLKLKLKNPGDYK
                     AISFEKLDTITEADTLKLALDYYNMFVGFSADSQKIKQAMAAINERLATPGLDTNIVY
                     YSIIHKYSHLGLDNEKLELEVRFILDKNFKVLHYDRGIQGQAYFDVAIATDLSEYYKI
                     DRYELDGKPYSTSTVRHRLPYGKHTIRWWKADKMLMSDDYLPLVSYNKEFWLMDNAKD
                     TVPGIRILVISGDYIFTYNSKEEVEKSEKTLNAVL"
     gene            1716..3377
                     /gene="crtI"
                     /locus_tag="PK252_11035"
     CDS             1716..3377
                     /gene="crtI"
                     /locus_tag="PK252_11035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT15283.1"
                     /translation="MKDKFYPPKFNGSDFHCPYCGVYSHQTWFALYFIDGNSYRKIEN
                     SNICICFHCYKKSIWYEGNMSKSVLIVGAGLGGLATGLRLAARGYQVSFVEKNQQPGG
                     RLNQLKMNGFTFDVGPSFFSMPYEFEELVKDCGMALPFEFVELDPLYTVNFRGSNRSF
                     RLYKDIDKLSSQFEGIEPNFKAKFTSYLKKCEAIYNDTVDIAIKQNFDSIPAYLLALA
                     KVNPRHLSVLLKTFWAQTGSYFESKEARQIVSLIAFFLGRTPFDTNAVYTLLSHIEFS
                     YTGYYNVKGGMYAIVEALTEELKKRNASFHFDTEIVDYVGHDDKLTSVIDQNGQKWEA
                     DIFVINSDAAYFRGKVLKRKKYADVRLDKMSWTMGYLTFYIGINTKLPQVEHHNYFLG
                     NNYEEYAKDVMKNPGTLEKPYYYVNVLSKHNAQCAPDGCEALFFVCPAPNMQYKTDWS
                     DKQDIVNSIIADFSERINYDITKNIVFQKIYTPEDWQNQYNLYKGAGLGLSHKMLQIG
                     AFRPKNYDEVFKNTFYVGASTVPGAGLPMAIISSKLTAERVYAMW"
     gene            4004..6646
                     /locus_tag="PK252_11040"
     CDS             4004..6646
                     /locus_tag="PK252_11040"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT15284.1"
                     /translation="MEIPAKYNAIESEKHWYKYWMEHKLFKSVPDHREPYCIVIPPPN
                     VTGVLHMGHMLNNTLQDVLIRRARMSGKNACWVPGTDHASIATEARVVNKLAKEGINK
                     FEIGREKFLEHAWDWTHKHGGIILEQLKKLGASCDWDRTCFTMDEARYESVIKVFVDL
                     YNKGLIYRGVRMINWDPQAKTAVSDEEVNYTDVKSKLYYVKYYVADDNNKATSDFVVV
                     ATTRPETIMGDTAVCVNPNDERYAHLKGKKLVVPMVNRIVPVIFDEYVDPTFGTGALK
                     VTPAHDLADYELGMKHKLEVIDIFNDDGTLNEKAKVFVGEDRFRARELSVKMLEENNL
                     LAKVEDYNNKVGLSERTNAVIEPRLSTQWFLKMKDLSKPALDNVLNNNIKLHPHKFVN
                     TYKHWMENVKDWCISRQLWWGQRIPAYYLPDGDYVVAETAEEALKMAKQKTGNNNLQL
                     SDLTQDEDVLDTWFSSWLWPLSVFDGIRKPNNDEVKYYYPTNDLVTAPEILFFWVARM
                     IIAGYEYAGDKPFRNVYLTGIVRDKQRRKMSKSLGNSPEPLELIEKYSADGVRVGMLL
                     CSPAGNDILYDEALSEQGRNFGNKIWNAFRLVKGWKIDSNIPSPEHSKIAVEWFNNKL
                     NKAIADIDRMYDEYRISEALMAVYKLFWDDFSSWYLEMIKPEYQKPIDAATYKATVDY
                     LEKLLHLLHPFMPFITEEIWQLIDTRKEGESIMISQMPKAQGYNEQLLTDFDVIIEAI
                     AHIRNIRKTRNMSYKDKADLLIRDHNKNYNARFEPVLIKMAGLETVSITSEKVDNTDS
                     FMVLSTEFYLKTGITIDKEAESLKIKEELEYTKGFLASVMKKLSNERFVSGAPAQVVE
                     MEKKKQADAEAKIKALEERLAALN"
     gene            6797..7720
                     /locus_tag="PK252_11045"
     CDS             6797..7720
                     /locus_tag="PK252_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT15285.1"
                     /translation="MKNIDRRNFLKSSLIAGVGLAVLPSLAKGATKLSGSSLPDIASI
                     AGTNHFQSTIDAIEAIGGINKFIPRGSKVGLLINAPGWWTKPGSYTHTNIVLATLKLL
                     NDAGISDICYLIDPAAEFFSRSPLSEKYTSLISSIKKCSGNYVEVNIPNAVKVKKAKV
                     IKELLQVDKLINIPINKHHSGVLYTGCMKNMMGACSGDTNKSFHQSISKDVEDVEHLS
                     QCIVDINHIRKQDLCITDATEVLKTNGPFGPGEIAKINKVFAGSSPVAMDAYGCTLLD
                     YRPQDIRTNVLASEQGFGNYDLKKYSIFEKE"
     gene            7728..8618
                     /locus_tag="PK252_11050"
     CDS             7728..8618
                     /locus_tag="PK252_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15286.1"
                     /translation="MRYLIIFMLLSINLTNGIGAELKNILPGNKEIDDWQVVDSIRTI
                     KGNQLFDYIDGGAEIFVEYGFVQAVSATYKDNANRQLQAEVYEMADSAASYGAYSFYL
                     NSEGQNANAGQEAQIIDYYCVVRKGNCVIVISIPQADEGLFKSIEKFASAIASKYNQT
                     NAKPELVSKIEKASIALGHIKYIEGSVGLGNIYRFIPGSAIKFSTAVSFFIDKTKCLV
                     FDYNTPEEANNNAAMALQKMQQSNPDVAFTKTDNGFFFTDFKNNQVKALLYKNYLTFT
                     IGKDVEKAKIAEEKIVEVLK"
     gene            complement(8718..8918)
                     /locus_tag="PK252_11055"
     CDS             complement(8718..8918)
                     /locus_tag="PK252_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15287.1"
                     /translation="MKKLAVSILMVAFIAVVPVSYAYSQECAKKEQKCDKKDDKKCCK
                     KEEKAACCKKDSTAKAGKKPAK"
     gene            complement(9278..10753)
                     /gene="gltA"
                     /locus_tag="PK252_11060"
     CDS             complement(9278..10753)
                     /gene="gltA"
                     /locus_tag="PK252_11060"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT15288.1"
                     /translation="MYTPEYIKQQREEQWRFELRNKLKAKDRTDKERVVMPAQAPEVR
                     NKNHAEVNLGLSNEMAMAEASRCLDCVNPTCIEGCPVNINIPKFIKYIEAGKFNKAIE
                     VIKEANAFPAVCGRVCPQEVQCEAKCFYVQKLNKPAVAIGNLERFAADCEMCSANIFI
                     PKIENKINKKVGVIGAGPAGLAAAIDLAKYGYKVTIFEALHDFGGVLKYGIPEFRLPN
                     TIIDKEIDTLKKLGVEFVNNFIVGKTATIDDLKQEGYEAFFIGSGAGLPKFMNIPGEN
                     YIGVFSSNEYLTRVNLMGAHKEGFDTPVLFGKNVAVIGGGNTAMDSVRTAKRLGAERA
                     MIIYRRSFDEMPARLEEIHHAKEEGVEFMNLRNPVKYIADEQGRVKKIVLRKIELGEP
                     DSSGRRRPVEVAGSEYELDIDMAIVSIGVSPNPIVPKTVANLETTCWGTIVVNEETMQ
                     SSVPGLFAGGDIVRGGATVILAMGDGRRAAKGIHEYLQSKN"
     gene            complement(10773..11591)
                     /locus_tag="PK252_11065"
     CDS             complement(10773..11591)
                     /locus_tag="PK252_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT15289.1"
                     /translation="MNKIVEKEFLSEKVVRLVIESPRIAKKRKAGHFVIIKIGEKGER
                     IPLTISSADPVKGTITLIVQTVGVTSTKVSKLNVGDVITDISGPLGQATHIEKVGTVL
                     CAGGGVGIAPLLPIVEAMKQAGNRVITVLAARSKDLIILEKEIRACSDEVIIMTDDGS
                     YGEKGLVTKGMEDVINREKVDLAITIGPAVMMKFTSLLTKKYEIPTMASLNSIMVDGT
                     GMCGACRISVGGETKFVCVDGPEFDAHKVDFDELLMRLNTFKDAELIARNSYMQ"
     gene            complement(11726..12205)
                     /locus_tag="PK252_11070"
     CDS             complement(11726..12205)
                     /locus_tag="PK252_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT15290.1"
                     /translation="MTVITDYSATNQDKLDGFFHEAFQSCGFAYEPEGKHSNLRNIDE
                     FFVKTGGGFWVLMQENNVVGSAGLFIYNKEQLIGELKCLYLLPSIQGKGYGQLMMKKV
                     IEEAKHRKLHYLRLDVKQNAFKAINLYHKNSFYEIDRYNGNPNDVIFMELNLKNQSI"
     gene            complement(12202..12597)
                     /locus_tag="PK252_11075"
     CDS             complement(12202..12597)
                     /locus_tag="PK252_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT15291.1"
                     /translation="MNVFYNTIVFVHNLTVSKHFYEKIIGLNVEQSYETIVFFENHFV
                     IHEAEAIISTIYGQKQQTKEKQGQNNLLIYFETDDIHASYQNMVNNKVNIIHPIIKQQ
                     WGQQVFRFYDPDGHIVEIGEAMHLEYLKK"
     gene            complement(12700..13188)
                     /locus_tag="PK252_11080"
     CDS             complement(12700..13188)
                     /locus_tag="PK252_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15292.1"
                     /translation="MIRVFKIGISFLAAMLVFTNSKAQATKQGCREFHKSRFCWIPGA
                     DDFKQFGRSKSTVVEMHQTYKYEVVLPPNRDYKIGTCVEPRFLPLQFRIVNKETEEAI
                     YDNSTDDYVETVGFTVKGKPLTVIIEITPLSTKYKPKDMRDTRACAGIQILYQRLGKD
                     GF"
     gene            complement(13225..14106)
                     /locus_tag="PK252_11085"
     CDS             complement(13225..14106)
                     /locus_tag="PK252_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15293.1"
                     /translation="MKILKIAIILGVITSLTQCKCSHSKAPATLMELDSLHDSISDVS
                     YVLPAPDEMLSDIMSYTMTINPKLANSLENAKRYPDNKSQAINLGVYVADFAYLNLNK
                     NKTNALDYFKTIRELAIKLNVYEFIDESFFDRVQNNLTNNDSLILISRELYFNMSEIL
                     ENANRNNVFALISAGAIIETIYLSSYSVSNFNDCQAIIYKIFEQKYLLATMFEFVSMY
                     KKDPEFSSIISDLEKIKDILDKGSESDKLATIVKDKENHFKAESSELQYNVDEKLFNE
                     LKIAINEVRNNIVTTSK"
     gene            complement(14158..17154)
                     /locus_tag="PK252_11090"
     CDS             complement(14158..17154)
                     /locus_tag="PK252_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15294.1"
                     /translation="MDKLLLKYMMEIFAILAHQYPSLHFENVKDFIRTFILKNLLIDI
                     AEKNINLFYDFYEKYRKTEDEPPQAHLEALYAILTQIAPKISQKQRFQILINLILFEK
                     YLLKYTSNANDDINNYTDTLNIIVKKIQINLVDYINCRNFINQTLYNIPKQENLLLLA
                     GKLNINVSIKYLQHNNLKGQIYFLFIESSNLLLYYYKGPANLSTNNIPIFENYIYIFS
                     KGESIKGPDIEPVYYNQIFKEFISTSKQKLEMVVKDVEFRYKNSNNGIHRLSVKIESG
                     QLIGIIGRSGTGKSTMLNILNGRIKPQQGSITINGLNFAQNTQMPDGLIGYIPQDDLL
                     LEELTVFENLYINTQLCFSNLTPTELTDKVNICLQKLNLYNIKDLKVGSPLKQVISGG
                     QRKKLNMALEFVREPWILLADEPTSGLSSSDSEEIMYHLAEFTYEGRIIVVNIHQPSS
                     DLFKLFDKIIVLDTEGYPVYFGTPVDAIDYFNQHIQRYTSLSEHCTTCENVNPEAIFK
                     AMEEKQVDDFGQLTNERKLGPKQWHQLYLNNIPNIEERAKGNPLPRTHTHKPSIIEQF
                     IIFSKRNILSKLANKAYVLMAVLISPLLSALLAFLCRSGSAEGETAYSFGNNENMIAF
                     LFMAVTVALFVGLIISAEEIIRDRKILRRESFLKLSKASYLHSKITYLFILSAVQTLL
                     FVLISNLILEIKGMGLYFWLILFATSCFGNILGLLLSSIFKSVVVIYIMVPIVIIPQI
                     LLSGTIVNFSKLNKWVTSSDYVPLVGDLMVSRWAYEGLAVSQFQYNQYQKHYFDVEKQ
                     ESNVKYNYYFLLPEIEKKISNIIETQKISQENVEFIKNALMQLSVFYDINEIIKSLDS
                     VPSVDLITKTDDMLKKINNDLLNRFNALSREKDNITRKLINTMGSMDNLLRFKDLHYN
                     KNIAELLLQRQTLKTFYIEKNNMYRQMEPVYQIPFSKTGRAQFLASSKLIGAIEVDTF
                     TFNVIVIWIITLIVYLILFRYFLLNL"
     gene            complement(17167..18327)
                     /locus_tag="PK252_11095"
     CDS             complement(17167..18327)
                     /locus_tag="PK252_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15295.1"
                     /translation="MEVNEEKNYKVLVVDDNPKNVQVLGSLLKNVGYKVGFAMDGKQA
                     FDLLQKSSDFDLILLDINMPVMDGYEACKAIRAEERLKEIPIIFLTAFNEVENIVMGF
                     ELGAQDYVTKPFNTKELLMRVKTQLDLKQAKDTLKKSNEILDLKVKEKTLALEEAFKR
                     LEKTDALKTNFLKYVSQQLREPLNGIVSSINIIKAQEQSSAIKHLLDILNMSVTQLED
                     FASQAMLYTELNLHNYSLKTTNINPAKLIQYALLDLNDSLQANDIIVQIDNINTNYQI
                     SADRDLLYKALIFIFKGLIKIIENNNIINISVKNEGNEVACIFSTQGIKIPSELQAGT
                     FDPFTGNQQKNDFNANLYFYFAKQVVELHKAKIEFVENESPGFNICIIFNQI"
     gene            complement(18330..22502)
                     /locus_tag="PK252_11100"
     CDS             complement(18330..22502)
                     /locus_tag="PK252_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15296.1"
                     /translation="MTNNEIKEPTILVLDDDVAINELICLKLESSGLLVQGTTAVPEA
                     LTMLEHQRFDLLLIDYRLSDTTAVVVVNELKNRNIDIPYVVLTGFSDQKIAVEMMKLG
                     AKDLFIKDPQVFDLLPSAILKIIENENKRKGFEKLEQKYKKLFNSIRDGYVSVDFNDH
                     ILELNDTFVELTGYTKDELLEKNYSQISPQKWVEKEQQLIKDIVLKRGYSDLYLKKFI
                     RKDGVMITTEIRSYTINDENNQPEGFWSIIRDITERKKTEAALKESEEQYRGLVEVSN
                     NAVFINQNNMIVYANPAAIRLFGAKTADQILYKSPFDFFHPDYHEIIIKRIKSMLEEG
                     VTAPLIEEKIVRLDGKVLDVETTAAPFILKGEKALQVILRDITERKQMEEALRLSEER
                     FRLIANTIDEVFWITQSDFSKIIYVSAAFEKIWGIPVERLYNDVNVFDENMHPDDKIK
                     FKEMLNKMAEGKACETECRLILPDKKIKWLSIKMFPLLNNWNNKVAQCIGVAHDITAR
                     KEAELAIEYERYLMNELMEKVPDVIYFKDLQSRFIRVSTSFLKKVAPQYKSKEELIGL
                     TDFDLFSQEHAQQAFEDEQNIIKTGRPIIGLEEKETHFDGPPTWALTTKMPLYDVYNN
                     IIGTFGISVDITERKQIEEKLRESEVHYRTLADSGQVLIWTTGVDQKADYFNQRWLEF
                     TGHSLEQEIENGWMDNIYARDAERCSQIFTSAFEKKARFSMDYRLLRADGEYRWIQND
                     GAPRYNSKGEFIGYICHCLDITDRKQIEKAILESEQKYRELTDMLPIGIFETDIEGNL
                     TFINKTITDWIGIKLNETKEKIKFFNFVAPEEHNRIEENFKSYIETGKPLIGEYKAIN
                     QEKSEFIVSISTTEIKEDCKIIGLRGVITDITEQKNAETLKIAKESAEKASRIKSEFL
                     ANMSHEIRTPLNAIIGFSDILYNSIASEKQSSQVDAIRTSARSLLNIINDILDISKVE
                     AGMLTLQYSPVNIHKLIHEIETLFIHKANEKGIALIVEINKKIPQILVLDEVRLRQIL
                     FNLVGNAIKFTEKGSVTITVDQMVNDPKSKNIDLIITVEDTGIGIPKEQHEQIFEAFH
                     QQVGQNIKKYGGTGLGLTITKKLIEMMGGKIYVNSEFGIGSIFEVYLNDVEIHNSTGT
                     EKEKEEVDINTINFIDATMLVCDHNPMNRKLLVDMFEETSLMILEAEKGAEAIELANE
                     YVPDIILIDLSLSDMSGYDVMQAIRNNPKTKKIPLIAASSTIAEVKNKKDSNSLYDEF
                     LLKPYQMNNLIELLMNHLKYEIKQPKQVEIKEQVSAELTEQQKQELSVLINRLNEELL
                     PESIQILDSQMIDQFEAFGNKIIELGNVHSLSILHDYGKDICRYADSFDIMKVTETLK
                     KFPAIIENLKKL"
BASE COUNT         6531 a         4244 c         4333 g         7437 t
ORIGIN      
        1 gactggcaaa accaatataa cctatacaaa ggcgccggct tgggcttatc acacagcatg
       61 ttgcagatag gggcctttcg gcctaagaat tacgatgaga tatttaaaaa taccttttac
      121 gttggagctt ctactgtgcc gggtgcaggc ctgcccatgg ctatcatcag ctcaaaactg
      181 actgtcgaaa gggtttacgc cttggggtga tgatagtttc tgctcaaagc taaaaccagc
      241 taataaaaaa gaatacgact tagcgatttt tttttaacac aaggcaaagt aaactcttaa
      301 ttcttagccc gacatttaaa aaatgacccg agcgaccctg atactgtcaa actcttcttt
      361 agtaagctct aacgcatctt ttaccaaaaa ctgcactgga atacttgcaa aattggcata
      421 ttctattgat aaaggcactc ctgtctctga tttttggtca gtgaaaatgt aattgaaaaa
      481 gccagtcaaa acagagtcct tctgaacaac ttctctacaa atagcattag aactgttaaa
      541 gtcgtacccg gcagcatttt gcatagtcat tttgggcact tggttataca tggctatgat
      601 tttttgctta attacttctt tttcagaaga actgtacttg ccacacgaag caaaaaacaa
      661 cataaaaata gctaccgaaa taaatttcaa agtcttcatt ttataaaagg tttggttaat
      721 aattatggaa ttataaaaca gcattaagtg ttttttcact cttttcaact tcttcttttg
      781 aattgtaggt aaatatgtaa tcaccactaa taacaagtat cctgatgcct ggtactgtat
      841 ctttcgcatt atccataagc cagaattcct tgttgtaaga aacaagtggc aaataatcat
      901 ctgacatcaa cattttatcg gctttccacc acctgatagt atgcttacca tacggcaaac
      961 ggtgccttac agtgcttgtt gaataaggct tgccatcaag ctcgtagcgg tctattttgt
     1021 aatactcact taaatcagtg gctatcgcaa catcaaaata agcctgtccc tgtatgcccc
     1081 tgtcgtagtg caacacttta aagttcttat ccaggataaa cctcacttcc agttccagtt
     1141 tttcattatc taaacccaaa tgcgagtatt tgtggatgat agaataataa actatattag
     1201 tatcaaggcc tggagttgcc aacctttcgt taatagctgc cattgcctgt tttatctttt
     1261 gcgagtcggc cgaaaaacct acaaacatgt tataataatc caaggccaac ttcaaggtat
     1321 ctgcttcggt aatggtatca agcttttcaa aagaaatagc cttgtaatcc ccgggatttt
     1381 tgagttttag cttcaaaaag cttttcacct ctttttcagc aattgtttgt ggtgtttttg
     1441 tacatgccgt aacaaatact gcaaggcaca gaatgtaagc aatagtcttc atgttaagtg
     1501 tttttatggt taattttata tagataatga tttgattagc aatcataagt attttttgcg
     1561 acaaatgaaa tttatcacat caaaagtttt tattagtttt agatgaaaat tacctatgct
     1621 atcttttaaa agtaaataag agatagatga aagtggtaat ttttgagaga caatagttgg
     1681 gtgcaagata aacactctaa ataacaaaat ttattatgaa agacaaattt taccctccca
     1741 aatttaacgg aagtgatttt cattgtccat attgtggtgt ttattctcac caaacatggt
     1801 tcgcattgta ttttatagat ggaaattcct atagaaaaat tgaaaattca aatatttgca
     1861 tttgttttca ttgttataag aaaagcattt ggtacgaggg taacatgagt aaaagtgtat
     1921 tgattgtagg tgccggctta ggcggcttag ccaccggatt gcgcctcgca gcaagaggct
     1981 accaggtttc gtttgttgaa aagaaccaac aacctggagg cagattgaac cagttgaaaa
     2041 tgaacggttt tacttttgat gtagggccaa gcttttttag tatgccttat gaatttgaag
     2101 aacttgtcaa agattgtggc atggccctcc catttgagtt tgtcgagctc gacccgctat
     2161 acacggtcaa tttcagaggc agtaacagga gtttcaggct gtacaaagac attgacaagc
     2221 ttagcagcca gtttgaaggc attgagccca acttcaaagc caaatttacg tcgtacctga
     2281 aaaaatgcga agccatttac aacgataccg tcgatattgc cataaagcaa aacttcgaca
     2341 gcatccccgc ttacctgctg gcattggcca aagttaaccc cagacacctg tcggtattgc
     2401 taaagacctt ctgggcacaa acaggcagct actttgaatc aaaggaagcc cggcagatag
     2461 tgtcgcttat cgctttcttc ctggggcgca cgccttttga taccaatgct gtatataccc
     2521 tgttgtcgca tatcgaattc tcgtacaccg gctattacaa cgttaaaggc gggatgtacg
     2581 ccatagttga agctttgacc gaagaattga aaaaacgtaa tgcttctttt catttcgaca
     2641 ctgaaatagt tgattatgtt gggcatgacg acaagttaac ttctgtaatc gaccaaaatg
     2701 gacaaaaatg ggaagctgac atttttgtta tcaactcaga cgcggcctat tttaggggta
     2761 aagtgttgaa acgcaaaaaa tacgctgatg ttcgtttgga taaaatgagc tggactatgg
     2821 gatacctgac attttatata ggcataaata ccaagttgcc acaagtagag catcacaact
     2881 actttttggg caataactat gaagaatacg ccaaagatgt tatgaaaaac cccggaacac
     2941 ttgaaaaacc ttattattac gtcaatgtac tttcaaaaca taacgcccaa tgtgctcccg
     3001 atggttgcga agcattgttt tttgtgtgcc ctgcccccaa tatgcagtat aaaactgact
     3061 ggtcagacaa acaggatatt gtaaacagca ttatagctga tttttctgaa agaataaatt
     3121 acgacataac caaaaatatt gtctttcaga agatatacac gccagaagac tggcaaaacc
     3181 aatataacct atacaaaggc gccggcttgg gcttatcaca caaaatgttg caaataggtg
     3241 cttttcggcc taagaattac gatgaggtat ttaaaaatac cttttacgtt ggagcttcta
     3301 ccgtgccggg tgcaggccta cccatggcta tcatcagctc aaaactaact gccgaaaggg
     3361 tttacgccat gtggtaatat aacccaaacc cataacaata agcacaacgt agctggattt
     3421 ctttttcgta tagcttgcct atgtaatatt tggtagtaac ctgtccagac gcaaaagctt
     3481 tgcgttttct gaaaacgcaa agcttttaaa cctgctcaaa ccaaaccacg ccaagccttg
     3541 atgtgccatg ttaaacggcc ggaggccgaa ctaatacaag cgacgtagca ggatgctacg
     3601 gaccgctttt attggcaggc tacgatgaac gggggggtga acttcgaaac gtttatgttt
     3661 cgcgcacctt gagcgcttca atggctacct gtactttaaa agctgctgaa tacttctttc
     3721 tgttttcata aacactggta aatttattcg ttttttcaac ttaaccagta gtctgaattt
     3781 tggggagtat tatggtttgc attttttgga agtaattctt gagaagatat ttaatataac
     3841 taactggctt aaaagaacat ccataagcga aacttcagca ttgaacatac tttcatgtct
     3901 ttttgtaatt atcccttgtt ttatgttggc agaaccatgg atgtttcatc tgaaaatttt
     3961 taattttacc gctctttttt aataacgtca tttataaaga tatatggaaa ttcctgcaaa
     4021 atacaatgcc atagaatcag aaaagcattg gtacaaatac tggatggagc ataaactttt
     4081 caagtcagta cctgatcaca gagagcctta ttgcattgta atacctccac caaacgttac
     4141 cggggtactg cacatggggc atatgctcaa caacactttg caggatgtac ttatccgaag
     4201 ggcccgcatg agcggcaaaa atgcctgttg ggtacccgga accgaccatg cttcaatagc
     4261 tacagaagca agggtggtaa ataaactggc caaagaaggc atcaacaagt ttgaaattgg
     4321 tcgcgaaaag tttcttgaac atgcctggga ctggacccac aaacatggag gcattatcct
     4381 ggagcagttg aaaaaacttg gggcctcatg cgactgggac cgcacttgtt ttaccatgga
     4441 tgaagcccgc tacgaaagcg ttatcaaggt ttttgttgac ctatataata aagggcttat
     4501 atacagaggt gtccggatga ttaactggga cccgcaagct aagactgcgg tttcggacga
     4561 agaagtaaac tataccgacg ttaagtcaaa actttattat gtaaagtatt atgtagcaga
     4621 cgacaataac aaagccactt ctgactttgt tgtcgttgca accacccgtc ctgaaaccat
     4681 tatgggagat actgccgttt gtgtaaaccc caatgacgaa cgatacgctc atttgaaagg
     4741 caagaaactg gttgtaccaa tggtaaaccg cattgttcct gtaatatttg atgaatatgt
     4801 tgacccaacc tttggtaccg gggccttgaa agttacacct gcccatgacc ttgcagacta
     4861 cgagcttggc atgaagcaca agctggaagt aatagatatt tttaatgatg acggtaccct
     4921 gaacgaaaaa gccaaagtat ttgttggaga agacaggttt agagcccgcg aactttcggt
     4981 aaaaatgctg gaagaaaaca acctgcttgc taaagttgaa gattacaaca acaaagtagg
     5041 gcttagcgaa aggactaatg cggtgattga gccaaggctc tcgacccagt ggttcctgaa
     5101 aatgaaagac ctttcaaaac ctgctcttga caatgttttg aataacaata tcaaacttca
     5161 tccgcataaa tttgtaaaca cctataaaca ctggatggag aatgtaaaag actggtgtat
     5221 atcccgtcaa ttgtggtggg gacagcgcat cccggcttac tatctgcccg atggcgatta
     5281 tgtagtggcc gaaacagctg aagaagcttt aaaaatggct aaacaaaaaa caggcaacaa
     5341 caatctgcaa ttatctgacc tcactcagga tgaggacgta ttggatactt ggttttcatc
     5401 atggttgtgg cccctttcgg ttttcgacgg catacgcaaa cccaataatg acgaggtaaa
     5461 atattattat cccacaaatg acttggttac agcgcctgag attttgttct tttgggttgc
     5521 ccgcatgatc attgccggat atgaatatgc cggcgataaa ccattcagaa atgtatatct
     5581 gacaggtatt gtacgcgaca aacaaaggcg caaaatgtca aaatcgcttg gcaattcgcc
     5641 cgagcctctt gagctgattg aaaaatatag cgctgacggc gtacgtgttg gcatgttgct
     5701 atgttctcct gccggtaacg acatcttgta tgatgaagcc cttagcgaac aaggccgtaa
     5761 ctttggcaac aaaatatgga acgcattccg cctggttaaa ggatggaaaa tagacagtaa
     5821 tataccttca ccagagcatt caaagatagc tgttgaatgg tttaataata aactaaacaa
     5881 agccattgca gatatcgaca gaatgtacga tgaataccgc atttcagaag cattgatggc
     5941 tgtatataag ctattctggg atgatttttc atcatggtat ctcgaaatga ttaaaccaga
     6001 ataccagaaa cctatagatg ctgctacata caaggctacc gttgattacc ttgaaaagtt
     6061 gctccatctt ttgcacccat tcatgccatt cattaccgaa gagatttggc aactgattga
     6121 cacccgcaag gaaggtgaaa gcattatgat tagccagatg cccaaagcac aaggttacaa
     6181 cgagcaattg cttactgact ttgatgtaat aattgaagcc attgcccata tcaggaatat
     6241 tcgcaaaacc cgcaacatgt cttacaaaga taaagctgac ctgttgatac gcgaccacaa
     6301 caaaaactac aatgcaaggt ttgagcctgt tttgattaaa atggccggac tggaaaccgt
     6361 atccattact tctgaaaaag ttgataatac tgattctttc atggtgcttt caaccgaatt
     6421 ttatctaaaa accggtatta ccattgacaa agaagctgaa agcctcaaga taaaagaaga
     6481 attggaatat accaaaggct tccttgcttc tgtcatgaaa aaactttcta acgaaaggtt
     6541 tgtatcaggt gccccagccc aggttgttga gatggaaaag aaaaagcagg cagatgctga
     6601 agctaaaatt aaagcattgg aagaaaggct tgcagcactg aattgacaat acatcgtaaa
     6661 aatagccaga gccttgagtt tcaaggctct tttttttgac tttatttaac gaaaaccgta
     6721 acttgcttaa caggttttta acaatcgaaa gccttgtaga tcctactttt actacacata
     6781 attaacttat atgcctatga aaaacattga ccgccgtaac tttttaaaaa gctcccttat
     6841 tgctggtgtt ggactggcag ttcttccttc tttagctaaa ggtgccacaa aactttcagg
     6901 ctcatccctc ccagatattg cgagcattgc aggaacaaat catttccaaa gtaccataga
     6961 tgccattgaa gccattggag gaatcaacaa gttcattccg cgtggctcaa aagtaggatt
     7021 gctcatcaat gcccccggtt ggtggaccaa acccggctca tatacgcata ccaacattgt
     7081 ccttgctact ttaaaattgc ttaacgatgc aggtatatcg gatatttgct atttgataga
     7141 cccggcggct gaattcttta gccgctcgcc attatcagaa aaatatacct cactaatctc
     7201 ttcaattaaa aaatgctctg ggaactatgt agaagtaaac atccccaatg cggttaaagt
     7261 caagaaggca aaagtaatta aagaattgct gcaagtggac aaacttatca atataccaat
     7321 aaacaagcac cactcaggag tattatatac cggatgcatg aaaaatatga tgggtgcatg
     7381 ctctggtgat actaataaat cttttcacca aagcatttca aaagatgtag aggatgtaga
     7441 gcacctttca caatgtattg tggatatcaa ccatatcaga aaacaagatt tgtgcatcac
     7501 agatgcaaca gaagtattga aaaccaatgg cccatttggg ccaggcgaaa tagccaaaat
     7561 aaataaagtc tttgccggct cttcgcctgt tgctatggat gcttatggct gtactttgct
     7621 cgattacagg cctcaggata taagaactaa cgtacttgcc tcagagcaag ggtttggaaa
     7681 ctatgaccta aagaaatatt caatttttga aaaagaataa tacatctatg cgctatttaa
     7741 taatattcat gctgctttcc ataaacctaa ccaatggaat aggcgctgaa cttaaaaaca
     7801 tcctgcccgg taataaagaa attgatgatt ggcaggttgt agattctatc cgtaccataa
     7861 aaggcaacca gttgttcgat tacatcgatg gaggcgcaga aatatttgtc gaatatggct
     7921 ttgttcaggc tgtttctgca acatataaag acaacgccaa tcggcaactt caggctgagg
     7981 tttacgaaat ggccgattcg gctgcctctt atggggcata ttcattctat ttaaatagtg
     8041 aagggcaaaa tgccaatgca gggcaggaag cgcaaattat tgactattac tgcgttgtcc
     8101 gaaaaggcaa ttgcgtgata gttatttcta tccctcaggc agatgaagga ctttttaaaa
     8161 gcattgaaaa atttgccagt gccattgcca gcaaatacaa ccaaaccaac gctaaaccag
     8221 agttggtttc caaaatagaa aaggcttcta ttgctctcgg ccatatcaaa tacattgaag
     8281 gctcagttgg actgggcaac atatataggt ttatccccgg aagtgccata aaattcagca
     8341 cagctgtttc tttttttata gataaaacca aatgcctggt ttttgactat aacacacctg
     8401 aagaagctaa taataatgct gcaatggcat tacaaaaaat gcaacaatcc aaccccgatg
     8461 tagcttttac taaaacagat aatggattct tctttacaga ctttaaaaac aaccaggtaa
     8521 aagcgctgtt gtataaaaac tatctgacat ttaccattgg gaaagatgta gagaaggcta
     8581 aaattgcgga agagaagata gtggaggtgt tgaagtagaa gattatggaa gaatatagaa
     8641 gtgtatggaa gagaagagga agagaagagg aagagaataa aaacaagaaa gccagactaa
     8701 atctggcttt cttcatgtta tttagcaggt ttcttcccgg ctttagcggt tgaatctttt
     8761 ttgcagcaag cagctttttc ttctttcttg cagcatttct tgtcgtcctt cttgtcacat
     8821 ttttgttctt tttttgcaca ttcttgcgaa taagcataag agactggcac tacggctata
     8881 aaagccacca ttaatattga tacagctaac tttttcattg taataatttt taaaattaca
     8941 agtacaaagc taacacgtat ttgtttcatc tcaaacaacc cgaatgatta ttaactttaa
     9001 aataacagat tttaatatct tttattattt tattactatt gtccggtaaa aatatcagca
     9061 ctaaagtccg ttagcaagtt agagtttgta agagggtgtg ttcaaaaagt caaaagaagc
     9121 taaccgcaaa ggtcgctaat tttttttgca aagtacgcaa agtgttaata gtcaacattt
     9181 aaatatttgc gtattgtatt ctttcttttt gaactttgcg gttaatattt ctttttacac
     9241 acctcttgtt gaaattaggt taaagaatca atcaatttta gtttttgctt tgcaagtact
     9301 catgaattcc tttggcagcc cttcggccat ctcccattgc aaggattaca gtagctccgc
     9361 ctcttacgat gtctccgcct gcaaacaaac cgggaacact actttgcatg gtttcttcgt
     9421 ttactacaat agtcccccaa caagtagttt cgaggttggc aacagtttta ggtactatgg
     9481 ggttaggtga aactccaata ctcactattg ccatatctat atccagctcg tattctgacc
     9541 cggctacttc tacaggccta cggcgtcctg agctgtctgg ttcgccaagt tcaatttttc
     9601 tcaaaactat ttttttgacc ctaccttgtt cgtcagcaat atattttaca gggtttctga
     9661 ggttcataaa ctcaacacct tcttcttttg catggtgtat ttcttccaaa cgggcaggca
     9721 tttcgtcaaa cgaacgacgg taaataatca tggccctttc ggcaccaagc cttttggctg
     9781 tacgaacgga gtccattgct gtgttcccgc cgccaattac ggcaacgttt ttgccgaata
     9841 atacgggtgt gtcaaaacct tctttatgtg cccccataag atttacacgg gtcaggtatt
     9901 cgttagatga aaatacccca atataatttt cgccagggat gttcataaat ttaggcaatc
     9961 cggctccgct gccaataaag aaagcttcgt acccttcttg tttaaggtca tcaatggtag
    10021 ctgtttttcc aacaatgaag ttgttgacaa attcaacccc caactttttc agggtgtcta
    10081 tttctttgtc aataatggtg ttgggcagcc tgaactctgg tatcccgtat ttcaacacgc
    10141 ctccaaaatc gtgcaatgct tcaaatatgg taaccttata accatatttt gcaaggtcaa
    10201 tggcagctgc cagtccggct ggccctgcac caataacccc caccttttta ttgattttgt
    10261 tttcaatttt ggggatgaag atattggcgc tacacatttc gcagtctgct gcaaaacgtt
    10321 caagattgcc aatggcaaca gctggtttgt taagtttttg gacataaaag cactttgcct
    10381 cgcactgtac ttcctggggg caaacgcgtc cgcaaactgc cggaaatgcg ttggcttctt
    10441 taattacttc aatagccttg ttgaatttac cggcttcaat gtatttgata aactttggta
    10501 tgttgatatt taccggacag ccttcaatac aagtagggtt aacacaatca aggcatctgc
    10561 tggcttcggc catggccatc tcattagaca acccgaggtt gacttctgca tggtttttgt
    10621 ttcttacctc aggagcctgc gctggcatta caacccgttc tttgtcggta cggtcttttg
    10681 ctttgagctt attgcgtaac tcaaacctcc actgttcttc acgttgttgc ttgatatatt
    10741 ctggtgtgta cataattctt ttattttcaa tattattgca tgtaactatt tctggcaatg
    10801 agttcagcat ctttaaatgt atttaaacgc atcagcaact cgtcaaagtc cactttatgg
    10861 gcatcaaatt ctggcccgtc aacacaaaca aactttgttt cgccaccaac acttatacgg
    10921 catgcgccac acattcctgt tccgtccacc atgatagagt tgagacttgc catagtaggt
    10981 atttcatatt tcttggtaag caaactggtg aatttcatca ttacagcagg gccaatggtg
    11041 attgcgaggt caactttttc gcgatttata acatcttcca tgcctttagt taccaacccc
    11101 ttttcgccat agctgccatc atcggtcatg ataattactt cgtccgaaca tgccctgatt
    11161 tctttttcaa gaatgataag gtcttttgaa cgtgctgcta agactgttat aacccggttc
    11221 ccggcctgtt tcatagcttc aacaataggt aaaagcggag ctatacctac accccctccg
    11281 gcacataata cagtccctac tttttcaata tgagttgctt gtcctaaagg tccggaaata
    11341 tcagtaataa cgtcacctac attgagcttt gaaactttgg tgcttgttac gccaacggtt
    11401 tgtactatta gtgtaatagt accttttaca gggtctgccg aagaaatggt aaggggtatg
    11461 cgttcgcctt tctctccaat cttgatgata acaaaatgtc ctgcttttct tttctttgca
    11521 atgcggggcg attcgataac caatcgtaca accttttctg aaagaaattc tttttcaaca
    11581 atcttattca taaatatatt ttgacgtatt ctaatattct ataagcaaat tggtaagtta
    11641 actttaattc atttataaag gggtacaaat attgaaaaaa aattcaatat ctatataggt
    11701 taagccctta tttattttgc aaagtttata ttgattgatt tttcaaattt aactccataa
    11761 aaataacatc attgggattg ccattatatc ggtctatttc gtaaaaacta tttttatgat
    11821 atagatttat agctttaaat gcattttgct ttacatctaa tcttaggtaa tgaagctttc
    11881 ggtgcttagc ttcttctata acctttttca tcattagttg cccatagcct ttaccttgta
    11941 ttgatggcaa caaatacaag cattttagtt cgccaataag ttgttcctta ttgtaaataa
    12001 acaaaccagc tgaaccaact acgttatttt cctgcatcaa aacccaaaat ccgcctccgg
    12061 ttttcacaaa aaactcatca atgtttcgca ggttgctatg ctttccttca ggttcatatg
    12121 caaaaccgca tgattgaaaa gcttcatgaa agaaaccgtc aagtttatcc tggtttgtgg
    12181 cgctatagtc ggttatgacg gtcatttttt caaatattcc agatgcatag cttctcctat
    12241 ttctacaata tgcccatcgg gatcgtaaaa gcggaaaacc tgttgtcccc attgttgttt
    12301 aattatcggg tggatgatgt ttactttatt gtttaccata ttttggtatg aagcatggat
    12361 gtcatctgtt tcaaaataaa ttagaagatt attttgccct tgcttttctt tagtctgttg
    12421 tttttggcca taaatggtag agattatagc ctctgcttca tggattacga aatgattttc
    12481 aaagaacaca atggtttcat acgattgttc tacattaagc ccaatgattt tctcataaaa
    12541 atgctttgat actgttaagt tgtgaacaaa aacgatggta ttgtaaaaaa cattcattag
    12601 taaatttttt ttgaaaaatt tttaacctat aaaataaaaa cacactatat atctaatgta
    12661 gcattaaata tatagtgtgt ttgcaataaa atcatttttt cagaaaccgt ctttccccaa
    12721 gcgttggtaa agtatttgaa tgcctgcaca tgcccgggtg tcgcgcatat ctttgggttt
    12781 gtactttgtt gataatggag ttatttcaat gatcactgtc aatggcttgc ctttaactgt
    12841 aaaaccaacg gtttcgacat aatcatcagt actattgtca tatattgctt cttcggtttc
    12901 tttgtttaca attctaaatt gcaacggtaa gaaccttggt tcaacacaag tgccaatctt
    12961 ataatcacga ttgggaggaa gtacgacctc gtatttatat gtctggtgca tctcgactac
    13021 ggtgcttttt gatcttccaa attgtttgaa atcatcggct ccggggatcc aacaaaacct
    13081 gcttttatga aattcacgac aaccttgttt tgtagcttgt gctttactat ttgtaaaaac
    13141 aagcattgca gccagaaagg atattccaat tttaaaaact ctaatcataa tgtataattt
    13201 tcaaaaactg ttatggtaaa aagtttattt tgaagtggta acaatattgt tgcgcacttc
    13261 attgattgcg atttttagtt cattgaaaag cttttcatca acattgtatt gtagttcaga
    13321 gctttcagct ttaaagtggt tttctttatc tttaactatt gttgctaatt tgtccgattc
    13381 gcttcctttg tcaagaatat cttttatttt ttctaagtca gaaatgatgg aactaaattc
    13441 ggggtctttt ttatacatag aaacaaattc gaacatggtg gccaaaagat atttttgttc
    13501 gaagattttg taaatgatgg cctgacaatc attgaagttt gaaacgctgt aagatgaaag
    13561 ataaattgtt tcgataatgg ccccggccga aataagtgca aatacgttgt tcctgtttgc
    13621 attttcaagg atttcggaca tgttaaaata caattcgcgc gaaatgagta tcagtgaatc
    13681 gttattcgtc aggttgtttt gtaccctgtc gaagaaactt tcgtcaataa attcgtaaac
    13741 attgagcttt atggccaatt ccctaattgt tttaaagtag tcaagggcat tggttttgtt
    13801 tttgtttaag tttaaatagg caaaatcggc tacataaact cctaagttta tagcctgaga
    13861 tttgttgtcc ggatatcttt ttgcgttctc gagcgaattg gctaatttgg ggttaatggt
    13921 cattgtgtat gacatgatat ctgatagcat ctcatcgggg gctggcaata cataagaaac
    13981 gtcagaaatg ctgtcatgta gggagtctaa ctccattagt gttgcagggg ctttgctatg
    14041 gctacatttg cattgtgtta aagatgtaat tacacctaaa attattgcaa tcttaagaat
    14101 tttcatattg ataagtttta atttgctttt atttattagc gcttatttat tttaacatca
    14161 taaatttaat aaaaaatatc tgaaaagtat caaataaact attaatgtta ttatccagat
    14221 aacaattaca ttaaaagtaa aggtgtcaac ttctatagct cctattaatt ttgaacttgc
    14281 caaaaattgt gccctgcctg tttttgaaaa aggtatttgg taaacgggct ccatttgtct
    14341 gtacatatta tttttttcaa tataaaatgt ttttaacgtc tgtctttgca atagcaattc
    14401 tgcaatgttt ttgttgtaat gcaaatcttt aaaacggagc agattgtcca tcgagcccat
    14461 agtgttgatt aatttacgtg tgatattgtc tttttcgcgg ctcaatgcgt tgaagcggtt
    14521 gagcaggtcg ttgttgattt tttttagcat gtcatctgtt tttgttatga ggtcaacaga
    14581 cgggacgctg tcaagacttt ttataatttc attgatgtcg taaaagacag atagttgcat
    14641 taaggcgttt tttatgaatt caacgttttc ctggcttatt ttttgcgttt caataatgtt
    14701 gcttatcttt ttttcaattt cgggcagcaa aaaatagtag ttatacttca cgttgctttc
    14761 ttgtttttca acatcaaaat agtgtttttg gtattggttg tactgaaatt gcgaaacagc
    14821 taacccttcg tatgcccagc gagataccat taaatctcct actaatggta cgtaatctga
    14881 agaggttacc catttgttta atttgctaaa gttgactatt gttccgctca aaaggatttg
    14941 agggataata acaatgggta ccatgatgta aattactacc actgatttga atatggaaga
    15001 taacagaagc cctaatatat tgccaaagca agaagtggca aataatatta accagaaata
    15061 aaggcccatt ccttttattt caagtatcag gttgcttatc agtacaaaca ataatgtttg
    15121 cactgcacta agaatgaata aataagtgat ttttgaatgt aaatagcttg ctttgctaag
    15181 ctttaagaat gattcgcgac gtaaaatctt tctatctctt attatttctt cggcgctgat
    15241 aattagcccg acaaaaaggg caacagtaac agccataaat aaaaaggcta tcatattttc
    15301 gttgttgcca aatgaataag cagtttcgcc ttcagcgctt cctgagcggc acaaaaaagc
    15361 aagcaatgca gataatagag gtgaaataag gactgccatt agtacgtatg ctttgttggc
    15421 cagttttgac agtatgttgc gtttgctaaa aataataaat tgttcaatga tagaaggctt
    15481 atgggtatgt gtacggggta ggggatttcc cttcgctctt tcttcaatgt tagggatatt
    15541 gttgaggtat aactggtgcc attgtttggg gccaagtttg cgttcgttgg ttagttggcc
    15601 aaaatcatct acctgttttt cttccattgc cttaaatatg gcttcggggt ttacgttttc
    15661 gcaagtagtg caatgttctg aaagcgatgt gtatctttgt atatgttggt tgaaataatc
    15721 tatggcatca accggggtgc caaaataaac tggatatcct tcggtgtcta aaacaattat
    15781 tttatcgaac agcttgaaca aatcggatga tggttgatga atattaacta caataattcg
    15841 gccttcatag gtaaactcgg ccaggtggta cattatttct tctgagtcag acgatgataa
    15901 acctgaggtt ggttcgtcgg ctaacaatat ccagggttcg cggacaaact ccagggccat
    15961 gttgagcttt ttgcgttgtc cgccgctaat aacctgtttc agcggtgaac ctacttttaa
    16021 atcttttatg ttgtaaagat tgagtttttg caaacaaata tttactttgt ctgttaattc
    16081 ggttggggta aggttgctaa agcaaagttg ggtgttaata taaagatttt caaatacggt
    16141 caactcttca agcaataaat cgtcttgtgg gatataccca atcaagccat ctggcatttg
    16201 tgtattttgt gcaaaattta gcccgttaat tgttatagac ccttgttgtg gttttatgcg
    16261 gccattgaga atgttgagca tggttgactt gcctgtgcca ctgcgaccga taatgcctat
    16321 tagttgtcct gattctattt tgacagacag gcggtgtatc ccgttgtttg agtttttgta
    16381 cctaaactca acatctttga caaccatttc gagcttttgt ttactggtag aaatgaattc
    16441 tttgaaaatt tgattgtagt aaaccggctc aatgtcaggt ccttttattg actcaccttt
    16501 gctaaagatg tatatatagt tttcaaaaat agggatgttg ttggttgaga ggtttgccgg
    16561 gcctttgtaa taataaagca aaagattgct gctttctata aataaaaaat aaatttggcc
    16621 tttcagattg ttgtgctgga ggtatttaat actgacgttt atattaagtt tgcctgctaa
    16681 caataacagg ttttcctgct tggggatatt atagagtgtc tggttgatga agttgcggca
    16741 gttgatataa tcgaccaggt tgatttgaat tttcttgaca attatattta gcgtgtcggt
    16801 ataattgttg atgtcatcat tggcattgct ggtgtatttg agcaaatatt tttcaaaaag
    16861 gatgaggttt ataaggattt ggaaacgctg tttttgagat atttttgggg caatttgggt
    16921 aagaatggca tacaatgctt caagatgtgc ttgtgggggt tcgtcttctg tttttcggta
    16981 tttttcgtaa aagtcataaa acaggttgat atttttttca gcgatatcaa ttaacaggtt
    17041 ttttagtata aaagtgcgga taaaatcttt tacgttttcg aaatgtaatg atggatattg
    17101 gtgagccaat attgcaaaga tttccatcat gtatttcagt aagagtttat ccattggttt
    17161 tttatactat atctgattaa aaataatgca tatattgaaa cccgggcttt cattttctac
    17221 aaattcaatt tttgctttat gaagttcaac tacttgtttg gcaaaataaa aatagaggtt
    17281 ggcattgaaa tcattttttt gctgattccc tgtaaaaggg tcaaaagtgc cagcctgaag
    17341 ctctgaaggg atttttatgc cttgagtgga aaaaatgcag gctacttcgt tcccttcgtt
    17401 ttttacggaa atattaataa tattattgtt ttcaataatt ttaatgagtc ctttaaagat
    17461 aaaaataagc gctttgtaaa gcaaatcgcg atcggctgaa atctggtaat tggtgttgat
    17521 attgtctatt tgtactataa tgtcgttggc ctgcaggctg tcgttgaggt cgagcaaagc
    17581 atactggata agttttgccg ggtttatatt tgttgttttt agtgaatagt tgtgcaggtt
    17641 aagctcggta tataacattg cctgagaggc aaagtcttct aattgcgtta ccgacatgtt
    17701 gagaatatct aataaatgct ttattgcaga ggattgttcc tgggctttaa tgatattgat
    17761 agatgaaact atcccattta atggctctcg aagttgttgg ctgacgtatt ttaaaaagtt
    17821 tgtttttaaa gcgtctgttt tttctagtct tttaaaggct tcctccaatg ccagtgtttt
    17881 ttcttttacc ttcaaatcaa gtatctcatt gctttttttt agtgtgtctt tagcttgctt
    17941 gaggtcgagc tgggtcttga ccctcatcag aagctctttg gtgttgaatg gtttggttac
    18001 ataatcctgg gcaccaagtt caaaacccat gactatattt tcaacttcgt tgaaagcggt
    18061 taaaaagata atcgggattt ctttgaggcg ctcttcagca cgaatggctt tgcaagcctc
    18121 gtaaccgtcc atgacgggca tgttaatgtc aagcagtata aggtcaaagt ctgaagattt
    18181 ttgaagcaaa tcaaaggctt gttttccatc cattgcaaaa cctaccttat atcccacgtt
    18241 tttgagcaga ctgcccagta cctgcacgtt tttggggtta tcatcgacca ccagtacttt
    18301 atagtttttt tcttcattaa cttccattgc tacaactttt tgaggttttc aatgatggca
    18361 gggaattttt tgagtgtttc ggtaactttc ataatatcaa aactatcggc atatctgcat
    18421 atatctttgc cataatcgtg caatatactt aaagaatgaa catttcctaa ttcaataatt
    18481 ttatttccaa aagcttcaaa ctgatctatc atctggctgt caagtatctg tatgctttca
    18541 ggcaataatt cttcatttag cctgtttatt aatacggaaa gctcttgttt ttgttgctct
    18601 gtcaattctg cagatacttg ttctttgatt tcaacttgct tgggttgttt tatttcatat
    18661 ttcaggtggt tcattaataa ctctatcagg ttgttcattt ggtatggttt gagcaagaac
    18721 tcgtcataca agctattgga gtctttttta tttttaacct cagcaatggt agaggatgct
    18781 gcgatcaacg gtattttttt tgttttgggg ttattccgta tggcctgcat cacatcataa
    18841 ccgctcatat cgctgagcga aaggtcaatt aatataatat ccggcacata ttcattggcc
    18901 agttcaatag cttctgcccc tttttcggct tccaaaatca tcaatgaggt ttcttcaaac
    18961 atatcaacca gcagtttgcg gttcattggg ttatggtcgc atacgagcat tgtagcatcg
    19021 atgaagttta ttgtattgat gtcaacttct tctttttctt tctcagtgcc tgtgctattg
    19081 tgtatttcta catcattgag atatacttca aaaatgctgc ctatgccaaa ctcgctattg
    19141 acatatattt ttccgcccat catttcaatg agttttttgg tgatggtaag gcctaagccc
    19201 gtacctccgt attttttgat attctggcct acttgttggt gaaaggcttc aaaaatctgt
    19261 tcatgttgtt ctttaggtat gcctatgccg gtatcttcaa cggtaataat caggtcaatg
    19321 tttttgcttt tggggtcatt gaccatttga tcgacagtaa tagtaacact gcctttttca
    19381 gtaaacttaa tggcatttcc gacaaggttg aacagtattt gtcgaagcct gacttcgtca
    19441 agtacaagta tttgggggat ttttttgttt atttctacaa tcagtgcaat gcctttttca
    19501 ttggctttat gaataaacaa ggtttctatt tcgtgaataa gcttatggat attgacaggg
    19561 gaatattgga gggtcaacat gccagcttct acttttgaaa tatccagtat gtcattaata
    19621 atgtttagca aactgcgggc gcttgtgcgt atagcgtcaa cctgcgagct ttgtttttcg
    19681 gaggcgatgg agttgtataa aatatcagag aatccaatga ttgcattgag cggggtgcgt
    19741 atttcatgcg acatattggc caagaattcg ctttttatgc ggcttgcttt ttcggcagat
    19801 tctttggcta ttttcagggt ttctgcattt ttttgttcgg taatatctgt aataacgcca
    19861 cgtaagccta ttatcttaca atcttctttg atttcagttg tagaaatgct gacaatgaat
    19921 tcactttttt cttgattgat ggctttatat tcgcctatca agggttttcc ggtctcgatg
    19981 tatgatttaa agttttcttc aatccggtta tgttcttcgg gcgccacaaa attgaaaaat
    20041 tttatttttt cttttgtttc gttaagtttg atgcctatcc aatcggtaat cgttttgttg
    20101 ataaaagtaa ggttgccttc aatatcggtt tcaaaaattc caatcgggag catatctgtg
    20161 agctcccgat atttctgttc actctcgagt atggcttttt ctatttgttt acggtctgtt
    20221 atgtcaaggc aatggcaaat gtaacctata aattcgcctt tgctgttata tcttggggcg
    20281 ccgtcatttt gtatccatcg gtattcgccg tcagccctta acaatcggta gtccatgcta
    20341 aaacgggcct ttttttcaaa tgcagaggtg aatatttggc tgcacctttc tgcatcccgg
    20401 gcgtaaatat tatccatcca tccgttttca atttcttgct ctaatgagtg gccggtaaat
    20461 tcgagccagc gttggttgaa atagtcggct ttctggtcaa cccctgttgt ccagataagt
    20521 acctgacctg aatcggcaag ggtccgatag tggacttcgc tttctctaag tttttcttct
    20581 atttgttttc gttcggtgat atctacagaa atgccaaatg tgccaataat attgttgtaa
    20641 acatcataca atggcatttt tgtagttaat gcccaagttg gcggcccgtc aaaatgggtt
    20701 tctttttctt caaggccaat gatgggcctg cctgttttaa ttatattttg ttcgtcttca
    20761 aaggcttgtt gggcatgttc ctgactaaat aagtcaaaat ctgtaagccc tatcaattct
    20821 tctttgcttt tatattgggg ggccactttt ttaagaaaag aggtgcttac tcttataaac
    20881 cggctttgca agtctttgaa gtaaatgaca tcggggactt tttccatcaa ttcgttcatc
    20941 aagtaccttt cgtattcgat agccaactct gcttctttcc ttgcagtaat atcgtgtgca
    21001 accccaatac attgagcaac tttgttgttc caattattta gcaagggaaa catttttatc
    21061 gataaccatt ttatcttttt gtctggcaat ataagcctgc attcggtttc gcaagctttc
    21121 ccttcagcca tcttgttaag catctccttg aatttgattt tatcatcagg atgcatgttt
    21181 tcatcaaaaa cattgacatc attgtataac ctttcaaccg ggatgcccca aattttttcg
    21241 aaggctgcgc taacatatat aatttttgaa aaatcagatt gagttatcca gaatacttca
    21301 tcaatggtat tggctatcaa tcggaaacgt tcttcgctta atcttagagc ttcttccatt
    21361 tgcttgcgtt ctgttatgtc gcgaaggata acctggagag ctttttcccc ttttaatata
    21421 aaaggggctg cagtagtttc aacatccaaa actttgccgt caaggcgcac gattttttct
    21481 tcgatcaagg gggctgtaac gccttcctct aacattgatt ttattctttt aataataatc
    21541 tcgtggtaat cgggatgaaa gaagtcaaac ggagatttgt ataatatctg atcagcagtt
    21601 tttgccccaa acaacctaat agcagccggg ttggcatata ctatcatatt gttttggttg
    21661 ataaaaacgg cattattaga cacttctacc aagccccggt attgttcttc gctttctttc
    21721 agtgcagctt ctgttttttt acgttctgtg atatcgcgta taatagacca aaaaccttcg
    21781 ggttggttat tttcatcgtt tatggtataa cttcggattt ctgtggtgat catcacgcca
    21841 tctttgcgga tgaatttttt caggtataaa tcggagtatc ctcttttgag tacaatatct
    21901 ttgatgagtt gttgttcttt ctcaacccat ttttgaggag atatttgaga gtaatttttc
    21961 tccagcagtt cgtcttttgt gtagccggta agttcaacaa aagtatcatt aagctcaagg
    22021 atatggtcgt tgaagtcaac gctaacatat ccatccctga tactattgaa caacttcttg
    22081 tatttttgtt ctaacttttc aaagcctttt cttttatttt cattttcaat gatcttgagt
    22141 attgccgaag gtaataagtc gaatacctgg gggtctttaa tgaataaatc tttggcccca
    22201 agtttcatca tctctactgc aattttttga tcgctaaaac cggtcaaaac aacataaggg
    22261 atatctatat ttctattttt caattcatta accactacta cagcagtagt atctgacaac
    22321 ctgtaatcaa taagcaaaag gtcaaatctt tgatgttcaa gcatagtcaa ggcttctggt
    22381 actgcagttg tcccttgaac taaaagcccg ctactttcta atttcaggca aataagctca
    22441 ttaatagcta catcgtcatc taataccagg atggtaggtt cttttatttc gttatttgtc
    22501 aagtttcaca aggttgttta tgataactta attgcaaaga taaaa
//
LOCUS       DAOSPF010000059        22073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2355_length_22073_cov_6.775865, whole genome shotgun sequence.
ACCESSION   DAOSPF010000059 DAOSPF010000000
VERSION     DAOSPF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_22073_cov_6.775865"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..599)
                     /locus_tag="PK252_11105"
     CDS             complement(204..599)
                     /locus_tag="PK252_11105"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT15297.1"
                     /translation="MKLLRIESTKFTPGIILDPGQNVLEFYGFSLPENAIEFYAPVIS
                     WLQEYKELLSNSLETLPQVQVFFKMVYYNSASLRQLVEIFQNFAEIYHMGIPVNVQWQ
                     YDGEDPSMAENGKELGEIAKIPVNVVAFN"
     gene            complement(689..2218)
                     /gene="guaA"
                     /locus_tag="PK252_11110"
     CDS             complement(689..2218)
                     /gene="guaA"
                     /locus_tag="PK252_11110"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT15298.1"
                     /translation="MVEKIVILDFGSQYTQLIARRVRELNVYSEIYPYNHFPQLDESV
                     KGVILSGSPFSVREANAPKPDLSSIRKKFPLLGICYGAQYLAQLLGGEVEASKTREYG
                     RANLSFINTNNPLLTKLSANTQVWMSHGDSITKIPADFEIIGSTNDVKVGAFQIKGEN
                     TFGIQFHPEVYHSTEGSVLLSNFVRNICGCHGEWTPQSFVESTIKSLQEQVKNDKVVL
                     GLSGGVDSSVAALLLHKAIGKNLTCIFVDNGLLRKDEFEKVLHSYKDLGLNVIGADAK
                     NKFLSELKGVSDPEKKRKIIGRCFIEVFDEEAHKITDVKWLAQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMKLKIVEPLRLLFKDEVRRVGRTVGLPEDLLNRHPFPGPGL
                     AIRILSDITAEKVAILQEVDSIFIEGLKSNDLYNKVWQAGAILLPVQSVGVMGDERTY
                     ENAVVLRAVTSTDGMTADWAHLPYEFLAKISNEIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            complement(2317..3099)
                     /locus_tag="PK252_11115"
     CDS             complement(2317..3099)
                     /locus_tag="PK252_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT15299.1"
                     /translation="MKVTFLGTGTSQGVPVIACDCNVCCSTNKHDKRLRSSVMIETHG
                     INISIDCGPDFRTQMLRENVKHLEAIVFTHEHKDHIGGLDDIRSFNYISGKPMDVYAE
                     SRVQDTIKNEFSYAFAKVKYPGVPEIMLHTIDENPFFIQNIPVTPIRLMHYKLPILGF
                     RIGNFAYLTDIKTISTEEKKKLIGCTHLAVSGLRHQTHLSHFTLKEAIGLIHEIKPAY
                     GYITHISHQAGLHSELNETLPPGINIAFDGLSFELDEPQLKS"
     gene            3286..4305
                     /gene="hypE"
                     /locus_tag="PK252_11120"
     CDS             3286..4305
                     /gene="hypE"
                     /locus_tag="PK252_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOT15300.1"
                     /translation="MGETKIITLNHGSGGKLTHDLIENLFLKYFKNKDIITLTDSAIV
                     PSQSSHLAFTTDGYVVEPIFFPGGNIGKLAVCGTVNDLAVSGAKPAYLSCSFIIEEGL
                     TFDELEKIVVTMAEEASKAGVNIVTGDTKVVHKGKGDKIFITASGIGFVDEDRAKIGS
                     ADKAKEGDKIIVTGSLGDHAIAILSSRESLTFETPVVSDCASLNGLIEKALEACPNIS
                     FMRDATRGGAATVLCELAEKTGKGIIIYEKEIPVKESVMAICEMFGFDPLFLANEGKA
                     VIVVPANDADKVLQILQMHELGKDAAIIGEITAENPRKVILKTSTGGKRWIDMPSGAQ
                     LPRIC"
     gene            complement(4440..6047)
                     /gene="xylE"
                     /locus_tag="PK252_11125"
     CDS             complement(4440..6047)
                     /gene="xylE"
                     /locus_tag="PK252_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HOT15301.1"
                     /translation="MLTLVATLGGLLFGYDTAVVNGAEKSLVEFYISKILDPSQLTYA
                     ISMITQYKILMSLVILLVFGIISGQIVKLLGTRKGLAISAGIIAIVVIWMISFLKKPI
                     PTEATALQDTADAIKGFVIASALIGCVIGGALSGFISRSLGRKNGLFIAAVAFFISAI
                     GAWKPEAFNIFGTLDVYSFVVYRIIGGIGVGIASMISPMYIAEIAPANSRGKLVSFNQ
                     FAIIFGMLVIYFVNYFIAKQGDEAWLITDGWRYMFLSGAIPAGIFIILLFFVPETPRF
                     LVLNDKEDKALKVLKKIAGEENAPKILEEIKGTLHEHHAPWLSYGFLIIFVGIMLSVF
                     QQFVGINVVLYYAGNIFRNMGASTDSSLLQTIIVGAVNLTFTVVAILTVDKFGRKPLM
                     IIGSIGMAIAMISLGFTFYWGQTGGQLTAAQGYAALFFMLFYTAAFAMSWGPVCWVLL
                     SEIFPNSIRGALSIAVAAQWIANWIVSLTFPMLNDNVWLTEKFNHGFAYWIYGIMGIL
                     SALFMWKFVPETKGKSLEEMDGLWKKK"
     gene            complement(6194..7507)
                     /gene="xylA"
                     /locus_tag="PK252_11130"
     CDS             complement(6194..7507)
                     /gene="xylA"
                     /locus_tag="PK252_11130"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOT15302.1"
                     /translation="MGNKEYFKGIGQIKFEGPTSKNPLAFKWYDANKVVGGKTMKDHL
                     RFAVAYWHTFCGEGGDPFGPGTINYEWNRAKDPMDLAKEKLDAAFEFISKLGVSFYCF
                     HDTDLVGDGSVFEIEKRLAIITEIAKKKQAESGINLLWGTANVFSNPRYMNGASTNPD
                     FAVLTNAAVQVKNAIDATIALGGTGYVFWGGREGYMSLHNTDMKRELEHMGKFLTLAR
                     DYGRAKGFKGTFFIEPKPMEPSKHQYDVDAQTVIGFLKQYGLEKDFKLNIEVNHATLA
                     QHTMEHELRVAADAGMLGSIDANKGDYQNGWDTDEFPTSITETTLAMLEILQAGGFTH
                     GGINFDAKTRRNSTDLEDIFIGHISGMDTFARALVIADKILADGDYLKMRKARYASFD
                     SGKGAEYEQGKLNFEDLYNIAKEIGEPKQISGKQELYEQLINLYI"
     gene            complement(7562..9046)
                     /locus_tag="PK252_11135"
     CDS             complement(7562..9046)
                     /locus_tag="PK252_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT15303.1"
                     /translation="MYLIGFDIGSSSVKASIIEASTGNCVASAFYPKKEMEIKAVQPG
                     WAEQDPEMWWENLKAATAEVLKESKASASDIKAIGISYQMHGLVTIDKNQKVLRPSII
                     WCDSRAVPYGEKAFNEIGGDKCLKHLLNSPGNFTASKLKWVKENEPEVYKQIDKIMLP
                     GDYIAMKLTGNIATSASGLSEGMFWDFTSNSVASFLMDYYGFDASFIPNILPTFSNQG
                     EVSAQAAAELGLAKGTPVAYRAGDQPNNAFSLNVLNPGEIAATAGTSGVVYGVSSEIK
                     YDPLSRVNTFAHVNHAKDSTRLGVLLCINGTGILNSWIKKNTGFADSTYEAMNEAAAS
                     IAIGSDGLSILPFGNGAERMLQNKNIGSLISNISFNIHSNKHMLRASQEGIVFAFHYG
                     MEIMKGIGINPSVIRAGNANMFLSPIFRDTLAGISGATIELYNTDGAQGAARGAGVGA
                     GIYKSFTEAFSGLKKLQTIQPDVAKKQQYAEAFENWKKALNKAI"
     gene            complement(9070..9792)
                     /locus_tag="PK252_11140"
     CDS             complement(9070..9792)
                     /locus_tag="PK252_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT15304.1"
                     /translation="MAVKLNPHVSVDCVIFGFDGQSLKVLLIDRNLGLTPEKQTAPNS
                     LKLPGSMVYDDEDVDTAAVRVLKELTGLENIYLQQLKVFGSPDRLSKKDDLDWLQKAT
                     RMTINRVVTVAYYALIKLHKAMPCEGKVIWQDTQNIPELAFDHRDIVIEGLTALRKEV
                     QYEPLVFELLPKKFTIRQLQTLYEIIEGRKFDNRNFRKSISRWQYLQPIDEKEKNVAH
                     KPAQFYRFDKRAYQRLKKEFFE"
     gene            complement(9987..10934)
                     /locus_tag="PK252_11145"
     CDS             complement(9987..10934)
                     /locus_tag="PK252_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15305.1"
                     /translation="MRKNIIILIITAFSLAIAATFLYFKNYSSTLNSNEAVFKIENNV
                     IVSKIEINKNKQNVFYLKSNKNGWSYCDKFSTDKTLMHFLFQIISTIEVSSLVSENIS
                     QTIADSLTKKGHEVIIYDVNENKIKDYFIWANNHNHTTYAIMKNSSQPYIVKIPGYQG
                     NFAGIFALSQEEWYNKSLIDFNPRDIKLISFENLSNRALSFAIDFSNGQIPVLIDAKG
                     QEVKYKKEAMQVYLNFLLKERLDMISFKNNIKQEKALYSIKIKTIQDKERSITFFQQI
                     PTNNTKPNTCLALINDNITASFNSNNLDLIFRDISFFIK"
     gene            complement(10944..12638)
                     /gene="gldG"
                     /locus_tag="PK252_11150"
     CDS             complement(10944..12638)
                     /gene="gldG"
                     /locus_tag="PK252_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOT15306.1"
                     /translation="MITNLKNSGHLVKLILYIVIIVSIYVISSFIYFRIDLTQEKRYT
                     LSEPTKQILQKLPGKIFIKVYLDGELPGGFTRLSRTVKETLDEFRIIAGDHIQYSFID
                     PYAGNDEKSIQQQIQELYKQGLEPVSVKIKDKKGNMAEKNIIPGAIASYNNIDFPINL
                     LSNNPGLSAEENLTHSEQSLEYSLINAIHCLAIRDNRKVAFIEGHGEYDQYQVADITR
                     ELASYFQVDRGEIHGNQSLLLPYSCIIIANPTKPWNEEDKFAVDQYIMQGGKVLWLID
                     PVSVARDSLVYGKTFANIAQLNLDDQLFRYGIRLNPVLVQDIQCNIIPVNTATSNTRP
                     QFSPAPWLYLPLLAGNQNSAISRNINLVKSEYTSYIDTLNIENIHFTSLLTSSARAKI
                     KTVPYIISMQEIKQQPALESFDKQFLPVAVLAEGTFTSVFKNRMLDDLQIVGSKKVIE
                     TSVYNKMIVIADADIIRNDVRYTNSQIYLGRIGRDKYTSQIFGNKDFILNAIQYLTDD
                     NGLISIRNKEVKMRLLNKIKLTEELRFWQAINIILPLVMVAILALIFFYIQKKRFI"
     gene            complement(12755..14083)
                     /locus_tag="PK252_11155"
     CDS             complement(12755..14083)
                     /locus_tag="PK252_11155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT15307.1"
                     /translation="MSKNFNIAILGCGTVGSGVAKILLDLDHDLHLRAQKKIVLKKIV
                     DLFPSKSAQQYQLPLNLFCGGGKDLTKEEASAFTKEILEDKEIDLVVETIGGTSDYLF
                     NVMISALKNGKHLVTANKAMLAERGEEIFNTAIENNVSLGYEASVCGAIPIIKAIRES
                     FTGDKVLEISGIMNGTSNYILSKMKEDGLSFDEALSMAQKAGYAEADPTLDISGGDAA
                     HKLTILLRLAFGAQVKYDHLYKEGINSLTKDDLDLANEMNCTLKLICFAKQTNQKIYA
                     AVRPMMVKNENILSKVNGATNCVEILNQYSGKHHFVGKGAGSLETASSIVGDIIFAAR
                     YDKQITNIPEEVSFELADADSVVLPYNITFVTEDYPGITGLVTTAIGRQQINIDTVSH
                     NRHTPEKSKAVFSIATMPCSLSQIKKAIADIKASAPGIMKEEPKIIPILN"
     gene            complement(14361..14780)
                     /gene="ndk"
                     /locus_tag="PK252_11160"
     CDS             complement(14361..14780)
                     /gene="ndk"
                     /locus_tag="PK252_11160"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT15308.1"
                     /translation="MAGNITFTMIKPDATARNLSGEILAMITKAGFKVKALKMTRLSK
                     AQAEGFYAVHREKSFFSELVEFITSGPVIAAILEKDNAVEAFRALIGATNPANAAEGS
                     VRKLYGTGVQENAIHGSDCDENAIIETNYFFSQLERF"
     gene            14870..15904
                     /locus_tag="PK252_11165"
     CDS             14870..15904
                     /locus_tag="PK252_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT15309.1"
                     /translation="MEYDINKDIRAVDALLNDKSPVLLVTHINPDGDAIGSTMAMYHY
                     LKKKGNKVWAMTPNDFPQFLAWVEASSDIIIFQKNKELAQAVIAEAKYIICIDFNGYA
                     RLKELGPLLEAANAMKVLIDHHPQPDNGFIYKIHDTKAPATAQLVFKFIKESGNIELL
                     DKTIATCIYTGMMTDTGVFSYNINNSETFRVIADLLDAGIDRDLIYDNVFNNFSHHRM
                     RLMGYCLNEKMVVLPHLHAAYIWLTKKEMEKFSFETGDTEGFVNMPFAIKGINITALF
                     TEKHDHVRVSMRSRGEFDVNKVCLKYFSGGGHKNAAGGESKAPIKEVIKEFEKILESY
                     RNEIQCFYHN"
     gene            15861..16400
                     /locus_tag="PK252_11170"
     CDS             15861..16400
                     /locus_tag="PK252_11170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT15310.1"
                     /translation="MSRTEMRYSAFIIISCFIGFLSCRQPVVQPNKNLPDPKKEHLIE
                     LNRAMVKQNELLIKAYIKRNAYKMVQTETGLWYGIYKKGKGPRIKVGTPVELKYTLSL
                     LDGTLCYSSDSLGMKKVKAGLGNVEAGLDEALLLMNIGDAAHIILQPHLAYGFSSDGN
                     RIPPTSIIVYDIEVFADHK"
     gene            16487..17527
                     /locus_tag="PK252_11175"
     CDS             16487..17527
                     /locus_tag="PK252_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799028.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOT15311.1"
                     /translation="MPRFNKLIIYYFSGTGNSKNVALWFSKVATERGIITLIVNIAEV
                     DRLHIETPPPDALIMFVSPIHGFNYPPVMMHFILRFPKGFNKVVLMNTRAGMLIGKWV
                     TPGLSGLAFYFASLVLKFKHYSIVGMMPVDLPSNWISLHPGLNERTVKFLHEKNKVRV
                     TAFAGKILAGKRCYKALKEIVQDLAISPISLLYYFVGRFVISKTFYASADCNNCDLCV
                     KNCPVRGIIKVNNRPFWTYRCESCMKCMSYCPQKAIETAHAPFAIFVVLYNLVLMVLF
                     YKYFGLLFFTIENVIVKFVVETVIFIVSLAVWYRLMHFLLRFHWFERLVVYTSLTKYT
                     WWGRRYKALKDN"
     gene            17579..18451
                     /locus_tag="PK252_11180"
     CDS             17579..18451
                     /locus_tag="PK252_11180"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT15312.1"
                     /translation="MLREQTHTYYEQKAELLLQYIANHLDGDLSVKTLSDISGISLFH
                     FHRVMKAALGETLGSYIDRVRLDTAVKLIRYGDISFTDIALRIGFKDLSAFSKAFTKE
                     FGISPNDFKADKNAVLNTHIDYKMTGTQKLVVDVKPKIKTLPDRMVYYIRVKGIYGGL
                     EVIKAWDELGDFIVMNKLDGWNLNLFSVYYDDGDVVGVENTISDVCIASKKMVSVSGR
                     IAVQNIPGGKYAVYRYKGPYDYLWDVYDAIFRDGVYPGDFKLRDAPIVEKYLNLSHKV
                     KPVNLLTEIFIPIE"
     gene            18448..19251
                     /locus_tag="PK252_11185"
     CDS             18448..19251
                     /locus_tag="PK252_11185"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOT15313.1"
                     /translation="MKFVLKYFTGTGNSLKILDTCKDAFIAKSHVASISAIKVGQQLP
                     QDADVIGFCFPVYAFGIPRICRQYLKELNRFKKQQKVFVLISAGDADEAGLAFRNCEK
                     ILTKKNCRVIYSAVVQMPVNWIIFEKAPSEKEAEAIIQKGVEFTYQVARDILNGVQKF
                     HQFNYPKRYSKFGFYKDYFLFKYLGIYNLCRMFKTYDSCNGCQLCSKICPTQSIVMVN
                     NRPTWTYKCEQCMRCVNFCPKGAIYQMYGGDTLGKDRYYEPSFKPLKEM"
     gene            complement(19254..20048)
                     /locus_tag="PK252_11190"
     CDS             complement(19254..20048)
                     /locus_tag="PK252_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT15314.1"
                     /translation="MISKLSFSYEPANIGSIIPFSKIFYKLGANTVHTFTYNYGLNSA
                     VAIAGTYGLVESGIDWEINKLSYENKFVAYSGMPAVAVGGLVPLAVPLGVYIYGRKHN
                     NTKLQITGLALGQAAILGAGLTTSIKVFTGRRPPGILRDDEEITDRSKDYSDDFAFGI
                     LRRGAFNGWPSGHTTTAFAMAITLTELYPENKALKIGAITYASIVGLGVSVNIHWFSD
                     AFAGALLGYAIGKTVGLSFKQLMNDEKPANYSLYVTPQGAGCVWRF"
     gene            20188..>20741
                     /locus_tag="PK252_11195"
     CDS             20188..>20741
                     /locus_tag="PK252_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOT15315.1"
                     /translation="MEKTIDQWQFEQSTTDFNRLMQKRIYRVLLVCSRYDYFILEEDG
                     RIEEQIFNEYMALNMRYPPAVIQTDSAEEALKILEDEETDLVILMLNIGESEPFSLAR
                     KIKDLYPGKPIVLLTPFLREVSAHLKHEDLSAIDYTFCWLGNADLLVAIIKLIEDKMN
                     AANDIINNGVQALLLVEDSVRYIS"
     assembly_gap    20742..20841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20875..>22073
                     /locus_tag="PK252_11200"
     CDS             20875..>22073
                     /locus_tag="PK252_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT15316.1"
                     /translation="MAFLFQSSDKSNAERARQLNAGFLWKQSKSLSHELKQYIIENFG
                     FGDLVFSNPKGKGETIRIHDLADLQKFIFDISDEAFIYHARRNEISRWLNARALFSIG
                     SLFKDLQIEDFETLNQARSCIFDAIAIYRITNCKGVIASFNKNDFNEYLTFCRIGEGS
                     LGGKGRGLAFLDALIRRHNLYDKFEKTHIVIPRTVVIATDLFDEFMEQNDLYKIASSE
                     SDDKKILEKFLKASLPNRLYHDLQNYVKKIQNPVAVRSSSKLEDSHFMPFAGVYRTYM
                     VPYSKDEKKMAEMLCDAIKCVYASVYYKESKAYMASTSSVIDEEKMAIILEEVCGKAY
                     QDRFYPAMSGVARSVNYYPVLPETAGDGVVNIAYGLGKYLVDGGSGMRFCPKYPKKAL
                     QLSVPELA"
BASE COUNT         6444 a         3906 c         4525 g         7098 t
ORIGIN      
        1 gaattgatat aatccagggt tctggttatt ttgaaactat tctcattgag aaaaggcacc
       61 tgggcgttaa tttttgcaaa agataagagt acaattaggt ttataaagaa gagctttttt
      121 ttattcattt taaaaaggtt aaaaaatgag gttaaaggat aatttcagag agatggttaa
      181 atttagaggt ttttattttg ctattaattg aaagcaacaa cgtttacagg tattttagca
      241 atttctccaa gctcttttcc attctcagcc atagatggat cttcgccgtc atattgccac
      301 tgaacgttga ccgggatgcc catgtgatat atttcagcga aattttgaaa aatttcaaca
      361 agctgtctta atgaagctga attgtaataa accattttga aaaaaacttg tacctgtggc
      421 aaggtttcga gtgaattgct aagcaattct ttgtattctt gcagccagga aataaccggg
      481 gcataaaatt caattgcatt ttcgggaagt gaaaaaccat aaaactcgag cacattctga
      541 cctgggtcaa gaattatgcc aggagtaaat ttggtagatt caattctaag cagcttcatt
      601 tattgcagta atatattcag ggcactaaaa gtaataatta ttcttcaaaa aaaaatattt
      661 ttcaaaaaat aagtaacggc gaaatgcctt attcccactc aattgtagca ggtggttttg
      721 aacttatgtc ataaacaacc ctgtttaccc cttttacttt gttgatgatt tcgttagata
      781 ttttagccag gaattcgtat gggaggtgtg cccaatcagc cgtcattccg tctgtagagg
      841 ttacagccct taatacaacg gcattttcat aagtacgttc gtctcccata accccgacag
      901 attgaacagg tagtaaaatt gcgccagcct gccatacttt gttgtaaaga tcattagatt
      961 taaggccttc gataaagata ctgtcaactt cttgcaaaat agctactttt tcagcagtaa
     1021 tatcgctaag gatacgaata gctaagccag ggcctgggaa aggatgcctg ttaagcaagt
     1081 cttccggtaa cccaacagta cggcctactc gtcttacctc gtctttaaat agtaagcgca
     1141 atggttctac tatcttaagt ttcatttttt caggcaatcc accaacgttg tggtgcgatt
     1201 tgatagtagc agaaggtcca ttgaccgata cagactcaat aacatcaggg tagatagtac
     1261 cttgggccaa ccattttaca tcagttattt tatgagcttc ttcatcaaat acttcaatga
     1321 aacagcgccc tataattttt ctttttttct caggatcaga aacgcctttt aattctgata
     1381 aaaatttatt tttagcgtca gcgccaatta cattcaagcc aagatcttta tatgaatgaa
     1441 gtactttttc aaattcatcc ttgcgtaaaa gtccgttatc aacgaaaata catgtaagat
     1501 ttttaccaat agccttgtgt aaaagtaatg cagctactga cgaatctaca ccgcctgata
     1561 aaccaaggac tactttgtcg tttttgactt gttcctgaag gcttttaatt gtactttcaa
     1621 caaatgattg gggggtccat tcgccgtggc atccgcaaat atttcttacg aaattgctca
     1681 ataatacaga gccttcagta gagtgataaa cttctgggtg aaactgaatg ccaaaagtat
     1741 tttctccttt gatttgaaaa gcgccgactt tcacgtcgtt ggtgctgcca ataatttcaa
     1801 aatcggcagg gattttggta attgaatcac catgcgacat ccaaacctgt gtattggctg
     1861 atagcttagt aagtaatgga ttgttcgtgt taataaatga caagtttgca cggccatatt
     1921 cgcgtgtttt tgaggcttct acttcgccac ctaacaattg tgccaggtat tgagctccat
     1981 agcatatacc aagtagtgga aatttcttac gaatgctgct caaatctggt tttggtgcat
     2041 ttgcttcacg cactgagaaa gggcttccgg ataagataac ccctttaacg ctctcgtcaa
     2101 gctgagggaa atggttgtaa ggataaattt cactgtaaac attgagttca cgaaccctgc
     2161 gggcaatcaa ctgtgtgtat tgtgacccaa aatcgagaat aacaattttt tctaccactt
     2221 gtatatgtat tgataattaa ctacttgaga ataattctac tgttgtacaa tttttctgca
     2281 aagatagaaa actatctgaa tgaattattg agtagtttag ctttttagct gaggttcatc
     2341 cagctcgaaa gacaaaccgt caaaagctat attaatccca ggaggaagag tttcatttag
     2401 ctcactatga agcccggctt ggtggctgat gtgtgtaatg tagccatagg ctggttttat
     2461 ctcatgtatc aaccctatgg cttcttttaa ggtaaagtgg gagaggtgtg tctggtgtct
     2521 caatcctgat acggcaagat gggtgcagcc tatgagtttt ttcttttctt ctgtgctaat
     2581 ggttttgata tctgtgaggt atgcaaagtt gccaattcta aaccctaaga tgggtaattt
     2641 ataatgcatc aagcgaatag gggtaacagg gatattttgg atgaaaaaag gattttcatc
     2701 aatggtgtga agcataatct caggcactcc aggatactta actttagcaa aggcgtatga
     2761 aaattcgttt tttatggtgt cttgtacacg agattcggcg taaacgtcca ttggtttccc
     2821 acttatgtaa ttaaaagacc gaatgtcatc aagtccgcca atatggtctt tgtgttcgtg
     2881 tgtaaataca attgcctcaa gatgctttac gttctcacgc agcatttgtg tccggaagtc
     2941 aggcccacaa tcaatagaga tgtttatgcc atgtgtttca atcattacag aagaacgaag
     3001 tcttttgtca tgtttatttg tcgagcagca tacattacaa tcgcaagcaa ttactggtac
     3061 gccttgggat gtgccggtgc caagaaatgt tactttcaat gttgtaaaaa ttattctatg
     3121 taaaagtaag gttttattta gaaatacgcg atattgttca tgtttaagta tatgtaaaga
     3181 aagtattggt tatttaaaac tggttaactt tgcgaggaaa taattacgga ttacgaatta
     3241 cggattaata ttaactaaca actaacaact aacaaccata ctattatggg cgaaacaaag
     3301 ataattacac tgaatcatgg aagtggggga aagcttactc atgatttgat tgaaaacctc
     3361 tttttgaaat actttaaaaa taaagatata attacgctga ccgattcggc cattgttcct
     3421 tctcaaagca gccatttagc ttttactaca gatgggtatg tggtggagcc tatttttttc
     3481 cctggtggaa acattggtaa gcttgctgtt tgcggtacgg taaatgattt ggctgtatca
     3541 ggggcaaaac cggcttattt aagttgttct tttattattg aagaaggatt aacctttgat
     3601 gaacttgaga aaattgttgt tacaatggct gaagaagctt ctaaagctgg agttaacatt
     3661 gttaccgggg atacaaaggt tgtgcataaa gggaaagggg ataaaatatt catcacggcc
     3721 tcgggaattg gatttgttga cgaggataga gctaaaatag gcagtgcaga taaggctaaa
     3781 gaaggggata agataatagt taccggttca ttaggggatc atgccatagc tatcctctcg
     3841 tcgcgcgaat cacttacttt tgaaactcct gttgtttcag attgtgcatc tttaaatgga
     3901 ttgatagaga aagccctgga agcttgtcca aatatctcat ttatgcgtga tgcaacaagg
     3961 ggtggggctg cgacagtctt atgtgagctt gcagaaaaga ctggaaaagg aataattatc
     4021 tatgaaaaag agattcctgt gaaagaaagt gtaatggcaa tatgtgaaat gtttggtttt
     4081 gatccgttgt tccttgccaa tgagggtaag gcggtaatcg tagttcctgc aaatgatgct
     4141 gataaagtgt tacaaattct tcaaatgcac gaacttggca aagatgctgc tattattggc
     4201 gaaataactg ccgaaaaccc gagaaaagta atactaaaaa cgagtacagg gggaaaacgc
     4261 tggatagata tgccatcggg ggcacagttg ccacgaatat gctaagaaaa ttagcgaatg
     4321 agataatttg aaaattagaa aatgggaaaa taaaaagagc tggtttttaa gtgaaaccag
     4381 ctcttttttt atttgtattt aattggctaa ttaacttatt ctcaaattcg ttaattatct
     4441 tatttctttt tccatagccc atccatttct tccagagatt ttcctttggt ttcgggaaca
     4501 aatttccaca taaacaatgc tgaaagtatg cccatgatgc catatatcca gtaagcaaaa
     4561 ccgtggttga acttttctgt aagccaaaca ttgtcattta acattgggaa tgtcaatgaa
     4621 acaatccagt tggcaatcca ttgtgcagca acagcaatag ataatgcgcc acggatagag
     4681 tttgggaata tttcggaaag caaaacccag cataccgggc cccagctcat ggcaaaagca
     4741 gctgtgtaaa ataacataaa gaacagtgct gcatatccct gtgcagctgt aagttgacca
     4801 ccagtttgtc cccaatagaa agtaaagcct aatgaaatca tagcaatggc catacctata
     4861 gaacctataa tcatcaaggg tttacggcca aatttgtcta ctgtaaggat agctacaaca
     4921 gtaaaagtaa gattaacagc cccaactatg atagtttgca gcaatgaact atcagtagat
     4981 gctcccatgt tgcggaagat gttgccggca taatataaaa ccacgttaat tcctacaaat
     5041 tgctgaaata cagaaagcat gatacctaca aaaataatta agaaaccata tgaaagccat
     5101 ggtgcatggt gttcatgaag tgtacctttt atttcttcca gtattttagg tgcattttct
     5161 tcacctgcaa ttttcttcaa tacttttagc gctttatctt ctttgtcatt aagcactaaa
     5221 aatcttgggg tttcggggac aaagaacagc aaaataatga aaatgccggc aggtattgcc
     5281 cctgaaagga acatataacg ccagccatca gtaattagcc aggcctcatc tccctgtttt
     5341 gcaatgaaat agttgacaaa atagataaca agcataccaa aaatgatagc aaactggttg
     5401 aaagatacca atttgccacg agagttggca ggggcaattt cggcaatgta cattggtgaa
     5461 atcatcgagg caataccaac ccctataccc ccgataatac ggtaaacaac aaaagaataa
     5521 acgtctaatg tgccaaaaat attaaaagct tcaggtttcc aagctcctat tgctgaaata
     5581 aagaaagcga cagctgcaat gaaaagtcca tttttacggc caagagagcg cgaaataaaa
     5641 cctgagagcg ccccgccgat gacgcatcca attagcgcgc tggctatgac aaagccttta
     5701 atagcatcag cagtatcttg taaggcagta gcttctgtag ggataggttt cttcaaaaaa
     5761 cttatcatcc agattactac aatagcaata atacctgcac ttattgctaa accttttctt
     5821 gtgcctaaaa gtttaactat ctggcctgat ataattccaa aaaccaataa aataaccaat
     5881 gacatcagta ttttatactg ggttatcatg gatatagcgt atgttaattg tgaagggtcg
     5941 aggatttttg aaatgtaaaa ttcgaccagc gatttttcag ccccgttaac aacagcggta
     6001 tcgtaaccaa aaagcaagcc tcctaaggtg gcaactaagg tcagcatggc tatatatagc
     6061 gggttcccct tgctataagt tgttgatgct gattttgatg aagtgtcaat aaaagccatt
     6121 agtatttgtt tttagtgtgg ttagtaaata aaaacccgga atttttaaaa ttccgggttt
     6181 aaatatagaa aaattaaatg tacaagttga taagttgttc gtaaagttct tgtttaccgc
     6241 taatttgttt tggttcacca atttctttag cgatgttgta caaatcttca aaattgagtt
     6301 tgccttgttc gtattcagcg cctttaccac tgtcaaatga agcataacgg gcttttctca
     6361 tctttaagta atcgccatca gctaaaattt tgtctgcaat taccaaagcg cgggcaaaag
     6421 tatccatacc actgatgtgg ccaatgaaaa tatcttcgag gtcggtagag ttacgacgtg
     6481 ttttagcatc gaagttgata cccccgtgtg tgaaaccacc agcctgaaga atttctaaca
     6541 ttgccaaagt tgtttcagta attgatgttg ggaattcgtc tgtatcccag ccgttttgat
     6601 agtcaccctt gttagcgtca atagaaccta acataccagc gtctgcagca acacgaagtt
     6661 cgtgttccat agtgtgttga gctaatgtag cgtggttaac ttcaatgttc aatttgaaat
     6721 ctttttccaa accatattgt ttcaagaaac caataacagt ttgagcatca acgtcatact
     6781 gatgttttga tggttccata ggttttggtt caatgaagaa tgttcctttg aaaccttttg
     6841 cacgtccgta atctcttgca agggtgagga atttacccat gtgttcgagt tcacgtttca
     6901 tgtcggtgtt gtgtaagctc atgtagcctt cacggcctcc ccagaataca taacctgtac
     6961 cacctaatgc aatagtagcg tcaatagcgt ttttaacctg gacggcagca ttggtcaata
     7021 cagcaaagtc aggatttgta gaagctccgt tcatataacg tgggttagaa aacacgttgg
     7081 ctgtgcccca aagcaggtta atgcctgatt cagcttgttt tttcttagct atttcagtga
     7141 taatggcaag acgtttttca atttcaaaaa cagaaccatc tccaactaag tcggtatcat
     7201 ggaagcaata aaatgaaacg cctaatttgc tgatgaattc aaaagctgca tcaagttttt
     7261 cttttgcaag atccatagga tctttagctc tattccattc gtaattgata gtgcccggac
     7321 caaatgggtc gccaccttcg ccacaaaatg tatgccagta tgcaactgca aaacgtaagt
     7381 ggtctttcat ggtttttcca ccaactactt tgttagcatc gtaccattta aatgccaatg
     7441 ggttttttga agtagggcct tcaaatttaa tttggccaat acctttaaag tactccttgt
     7501 ttccgattaa aacgctcata attaataaat taaaaatgtt aattaataaa ttcttcttat
     7561 tttatatagc tttgtttaaa gcttttttcc agttctcaaa agcttcagca tattgttgtt
     7621 ttttagcaac atcgggctga atagtttgta atttctttaa tcctgagaaa gcttcagtaa
     7681 aagatttgta aatacctgca cctacaccag ctccacgagc tgcgccttgt gcaccatcgg
     7741 tattgtagag ctcaattgta gcgccagaga tgccggcaag ggtatcacgg aatattgggc
     7801 ttaggaacat gttggcattg ccagctctga taacagatgg gttgatacca atgcccttca
     7861 tgatttccat tccgtagtgg aaagcaaaca caatgccttc ctgagaggct cgaagcatgt
     7921 gcttgttaga gtgaatgtta aagctaatgt tgctgattaa gctgccaatg tttttattct
     7981 gaagcatacg ttcagctccg tttccgaaag gtaatataga taaaccgtca ctaccaatag
     8041 caatagaggc agcagcttcg ttcattgctt cgtaggttga atctgcaaat ccggtatttt
     8101 tcttgatcca tgagttcaaa ataccagtgc cgttaatgca caacagaacg ccaagacgag
     8161 tactatcctt agcgtggtta acgtgggcaa aagtgtttac acgtgacaga gggtcgtatt
     8221 taatttcgct acttacacca tatacaaccc ctgaagttcc tgctgttgct gcaatttcac
     8281 cagggttaag cacgtttaaa gagaatgcat tatttggttg gtcgccagca cggtatgcaa
     8341 caggggtgcc ttttgctaat cccagctcag cagcagcttg agctgatact tctccctggt
     8401 ttgagaatgt tggcaatata tttggaatga atgatgcatc aaaaccatag taatccatca
     8461 gaaaagatgc aacagaattg ctggtgaagt cccagaacat tccttcagaa agaccagagg
     8521 ctgaagtggc aatgtttccg gtaagtttca tagcaatgta gtcgccaggg agcatgattt
     8581 tgtctatttg tttgtacact tcaggttcgt tttcctttac ccatttgagt tttgaagcgg
     8641 taaagttgcc tggggagttc aacaagtgtt ttaaacattt gtccccgcca atttcgttga
     8701 aggctttttc gccataagga acagcccggc tatcgcacca gataattgaa gggcgaagta
     8761 ctttctggtt tttatctata gtcaccaaac cgtgcatttg gtaagagata ccaatggctt
     8821 taatgtctga agcagaagct ttagattctt taagcacttc ggctgttgca gccttaaggt
     8881 tttcccacca catttcgggg tcttgttctg cccacccagg ctgaacagcc tttatttcca
     8941 tttctttttt aggatagaat gcagatgcta cgcagtttcc ggtagatgct tctattatac
     9001 tcgcttttac agacgagctt ccaatgtcaa aacctatcaa atacatgctg aatgtgttta
     9061 gtttataagt tattcaaaaa attctttttt taaacgctgg tatgcccttt tatcaaacct
     9121 gtaaaactgt gccggtttgt gggctacgtt tttttctttt tcatcaatag gctgtaaata
     9181 ttgccaacgc gaaatacttt ttcgaaaatt acggttgtca aattttcgtc cttcaatgat
     9241 ttcgtataat gtttgtaatt gtctgatggt aaatttttta ggcaatagtt caaagaccag
     9301 tggctcgtat tgcacttctt ttcgcaaggc tgtaaggcct tcaattacaa tgtcccggtg
     9361 gtcaaaggca agttctggta tgttctgggt gtcttgccaa attactttgc cctcgcaagg
     9421 catagcttta tgaagcttga taagagcata ataagcaact gtaacaacgc ggtttatggt
     9481 catcctggtt gctttttgca gccaatctaa gtcatctttt tttgaaagac gatcgggcga
     9541 accaaatact tttaattgtt gaaggtaaat attctcaagg cctgtaagct cttttaatac
     9601 acggacagca gctgtatcta catcttcatc atcgtaaacc atgctgccag ggagttttaa
     9661 tgagttcggg gcagtttgtt tttctggtgt aagtcccaag tttctgtcta tcaacaaaac
     9721 ttttaggctt tgtccgtcaa aaccaaatat gacacagtca acagaaacat gcggattgag
     9781 cttaactgcc ataaattatt tttttgtaaa tgtaatacat caaatatcaa aaatgcaaac
     9841 ttagcgtaaa aaatacgtta actattttat aaaaaaatgt atttcagtaa tttgtaaaga
     9901 atatttttaa attgctaaat tttgaaaaaa tataggtgat aactacgaag gtcttatgta
     9961 gtaaagcatt tatggttatt tttatgctat ttgataaaaa atgaaatgtc cctaaaaatt
    10021 aagtccaggt tattactgtt aaaactggcg gttatattgt cgttgatgag ggcaaggcat
    10081 gtgtttggct ttgtattatt tgttggtatt tgttgaaaga aagtaatgga gcgttcttta
    10141 tcttgtattg ttttgatttt tattgaatat aaagcttttt cctgtttaat gttattttta
    10201 aagctaatca tgtcaagtct ttcttttaat aaaaagttga gatacacttg catagcttct
    10261 tttttatatt ttacttcctg gccttttgca tcaattagta caggtatttg tccgttactg
    10321 aagtctatgg cgaacgatag tgcacggttt gaaaggtttt caaatgaaat caatttaata
    10381 tctcttgggt taaaatcaat taatgatttg ttgtaccatt cttcctgact taaagcaaat
    10441 attcctgcaa aattgccctg ataacctggt attttaacaa tgtatggctg gctactattt
    10501 ttcattatgg cgtatgttgt gtggttgtgg ttgtttgccc aaatgaaata gtcttttatc
    10561 ttattttcat ttacatcata aattatcact tcgtgtcctt tttttgtaag tgaatctgca
    10621 atggtttgcg atatgttttc agaaactaat gatgaaacct ctattgtaga aataatttga
    10681 aacaagaagt gcattaaggt tttgtctgta ctaaatttgt cacaatatga ccatccgttt
    10741 ttattgcttt ttaaataaaa aacattttgt ttgtttttat ttatttcaat tttgctgaca
    10801 atgacattgt tctcaatttt aaaaaccgct tcgttactat taagtgtgct tgaataattt
    10861 ttaaagtaca gaaatgtggc ggcaatagct aaagaaaagg ctgtaattat caggatgatg
    10921 atgttttttc tcatatcatg agtttatata aagcgttttt tttggatata gaaaaatatt
    10981 aatgctaaga tggctaccat aacaagtggt aaaattatgt ttatagcttg ccagaacctt
    11041 aattcttctg ttagtttgat tttgttaagt aatcgcatct ttacttcttt attccttatc
    11101 gaaataaggc cgttgtcatc agtcaggtat tgaattgcgt ttaaaataaa gtctttattg
    11161 ccaaagattt gtgaagtgta cttgtctcgg ccaatgcgcc ctaaatatat ttgcgagttg
    11221 gtatatctta cgtcatttct gataatgtcg gcatcggcaa taactatcat tttgttgtaa
    11281 acgcttgttt caattacttt tttgcttccg acaatttgta aatcatccaa catcctgttt
    11341 ttaaaaacag aagtaaacgt tccttcggca agaactgcta ccgggagaaa ttgtttgtca
    11401 aaactttcta aagctggttg ttgcttgatt tcttgcatag aaataatgta aggcactgtt
    11461 tttatctttg ctcttgccga tgatgttaat aagcttgtaa aatgaatatt ttcaatgtta
    11521 agtgtatcta tatagcttgt atattctgat tttacaagat tgatatttct tgaaatcgca
    11581 ctgttctgat tacctgccaa taaaggcaag taaagccagg gtgcagggga gaattgtggg
    11641 cgtgtatttg atgttgccgt attgacgggg atgatattac attggatgtc ttgcacaagc
    11701 acagggttta ggcgtattcc gtaacgaaac agctggtcgt caaggttgag ctgggctatg
    11761 ttggcaaatg tttttccata aacgaggctg tcccgggcta cagaaaccgg gtcgatgagc
    11821 cacagcactt tccctccctg cataatatat tggtcaacgg caaacttgtc ttcttcattc
    11881 catggttttg ttggatttgc tataataatg caactgtaag gtagtaatag tgattgattt
    11941 ccgtgtatct cgccccggtc aacttgaaaa tagctggcta actcgcgggt aatatctgca
    12001 acctggtact ggtcatattc tccatgacct tcaataaaag caactttccg gttgtcacga
    12061 atagcaaggc aatgaatggc atttatcaac gaatattcca ggctttgttc tgagtgtgta
    12121 aggttttctt ctgctgaaag cccgggatta ttgctaagca ggtttatagg aaaatcaatg
    12181 ttattgtaac tggcaatagc tccggggata atgttttttt cggccatgtt gccttttttg
    12241 tctttaatct ttaccgaaac aggctctaat ccttgtttgt atagttcttg tatttgttgt
    12301 tgaatgctct tttcatcgtt tccggcataa gggtcaataa atgagtactg aatgtggtct
    12361 ccggcaataa ttctgaattc gtcgagtgtt tcttttacag tgcggcttag tcgggtaaat
    12421 ccgccgggta gttcgccatc cagatacacc tttatgaaga ttttgcccgg caatttttgt
    12481 agaatctgct ttgtaggctc agaaagggtg tatcgtttct cttgtgtaag gtctatcctg
    12541 aaatatatga atgaagatat gacgtaaatg ctgacaatta taacgatgta caaaatcagt
    12601 tttacaagat gcccgctatt ttttaagttt gttatcattg tatgttttat agtaatgcaa
    12661 aataaaaaaa tattcttgtc acaatttact taaaggatac aaaaaaggca cataatgaat
    12721 gtttatgtgc cttttggtgt tatatcaatc ccgattagtt taaaataggg ataattttgg
    12781 gttcttcctt cataataccg ggagctgaag ctttaatatc tgcaatggcc ttttttattt
    12841 gcgataaaga gcaaggcatg gtggctattg aaaataccgc tttgcttttt tcaggtgtat
    12901 gcctgttatg gcttactgta tctatattta tttgttgacg acctattgca gtagttacta
    12961 atcctgttat tccaggataa tcttctgtaa caaatgtaat gttgtaagga agaacaacag
    13021 aatctgcatc ggcaagctca aaagaaacct cttcggggat attggttatt tgcttgtcat
    13081 aacgggcagc aaaaatgata tccccgacaa ttgaactggc tgtttcaagg cttcctgccc
    13141 ctttgccgac aaagtggtgt ttgcctgaat attggttcaa tatctcaacg cagtttgtgg
    13201 ctccgttcac tttagagaga atgttttcat ttttcaccat cattggcctt actgctgcat
    13261 atattttctg attggtttgt ttggcaaaac aaatcaattt gagagtacaa ttcatttcgt
    13321 tggccaaatc aaggtcatcc ttagtcagcg agtttatccc ttctttgtac agatggtcat
    13381 attttacttg tgccccaaaa gccaaacgca gcaatattgt tagcttgtgg gcagcgtcgc
    13441 caccactgat atcaagtgtc gggtctgctt cggcatagcc tgctttttgg gccatagaaa
    13501 gagcttcgtc aaaacttagc ccgtcttctt tcatttttga aaggatgtag ttgcttgttc
    13561 cgttcataat gccagaaatt tcaagcactt tgtcgccggt aaagctttct cggatggctt
    13621 tgataatagg tattgcccca caaacgcttg cttcgtagcc caggctgaca ttgttttcga
    13681 tggcagtgtt gaaaatttct tcgcctcttt cggccagcat ggctttattg gcagtaacaa
    13741 ggtgttttcc gttttttaat gctgagatca ttacgttaaa gaggtaatcg cttgtgccac
    13801 ctatggtttc aactactaaa tcaatttctt tgtcttcaag gatttctttt gtaaaagcag
    13861 aagcctcttc ctttgtaagg tctttgccgc ctccgcaaaa tagatttagc ggaagttggt
    13921 attgttgggc gcttttagat ggaaaaaggt caacaatttt ttttaaaaca atcttttttt
    13981 gggctctcag gtgaagatca tgatccaaat caagaagaat tttggcaaca ccgctgccta
    14041 ctgtcccaca gcccagtatt gctatattga aatttttact cattgttcta atttttaaat
    14101 gtaaactttg tgaaaattta gcagcttact gctgcaactg cctgattttg ctaaaacaag
    14161 gaaaacaaaa aaatatctcc gtaagataca aaacacactc aaccccccaa gggggtggta
    14221 gttgaagtgg tatatgttat gcacatcaga ggtgcaaagt tcgaagatat gttcatcttt
    14281 tttttattca caaaggtaaa agaatgtatc aaatgtcaac aaatagtatg ccgaaaacaa
    14341 tatccttttt cactgattat ttaaaaacgt tcaagttgtg aaaagaaata atttgtttcg
    14401 atgatggcat tttcgtcaca atctgagcca tgaatagcat tttcctgaac gccagtgccg
    14461 tagagcttac gtacagagcc ttctgctgcg ttggcagggt tagttgcccc tatcaatgcc
    14521 cgaaatgctt caacggcatt gtctttttca aggattgcag ctataaccgg cccggaagta
    14581 ataaattcaa caagttcgct aaaaaaactt ttttcgcggt gcacagcata aaagccttca
    14641 gcctgggctt ttgataaacg agtcatttta agggctttaa ccttaaaccc ggctttagta
    14701 atcattgcca aaatttcgcc cgaaaggttt cttgctgttg catcgggttt gatcatagta
    14761 aatgttatgt tacctgccat atacgctttg ttaattttgc gaatataaga aaatcggttt
    14821 tatttattaa tcatatccac tatttttaca aaaaattaaa atttgcaatg tggaatacga
    14881 tataaataaa gatattcggg cggtagatgc cctgttaaat gataaatcgc ctgttttact
    14941 ggtaactcat atcaaccctg acggggatgc tattggttct actatggcca tgtatcacta
    15001 tttgaagaaa aaaggcaata aagtttgggc tatgacaccc aatgattttc ctcaatttct
    15061 tgcctgggtt gaagcaagta gtgatattat tatttttcaa aaaaacaagg agcttgccca
    15121 ggctgtgatt gctgaagcga agtacattat ttgtattgat tttaatggtt atgccaggtt
    15181 aaaagagctt ggcccattgt tagaggctgc taatgctatg aaggttctta ttgaccatca
    15241 tccgcaacct gataatggct ttatttataa aattcatgat acaaaagctc cggcaacagc
    15301 acaacttgtt tttaaattta tcaaagaatc cgggaatatt gagcttttgg acaaaacgat
    15361 tgccacatgc atttatactg gcatgatgac cgatactggc gtattttcat acaatatcaa
    15421 caattcggaa acgttcaggg taatagcaga tttgttagat gccggtattg acagggattt
    15481 gatatatgat aatgtgttca ataacttctc gcatcaccgc atgcggttga tgggctattg
    15541 tctgaacgag aaaatggtag tgttgcctca tcttcatgct gcatatatat ggctgacaaa
    15601 gaaagaaatg gaaaagttct cgtttgaaac aggagatacc gaaggatttg taaatatgcc
    15661 ttttgccata aaaggaatta atattacagc tttgtttact gaaaaacatg accatgtgag
    15721 ggtttctatg cgttcgcgtg gcgaatttga cgtaaataaa gtatgtttaa aatatttttc
    15781 tggcggaggc cataaaaatg ctgccggagg agaatctaaa gctccgataa aggaggttat
    15841 taaagagttt gaaaaaatac ttgagtcgta ccgaaatgag atacagtgct tttatcataa
    15901 ttagttgttt tattggtttt ctctcgtgca gacagccggt tgtccagcct aataaaaacc
    15961 tgccagaccc taagaaagaa caccttattg agctcaaccg tgccatggta aagcaaaatg
    16021 aattgctcat taaagcttat atcaagcgaa atgcttataa aatggtgcaa actgaaaccg
    16081 gtttgtggta tgggatatat aaaaagggca aagggccgag gattaaagtt ggaactccgg
    16141 tagaactcaa atacacatta agcttgcttg acggaacgct ttgttattca tcagattcgt
    16201 tagggatgaa gaaggttaag gccgggcttg gcaacgttga ggctggtctt gacgaagcct
    16261 tgttgcttat gaatattggt gatgctgccc atataatatt acagccccat ttggcttatg
    16321 gcttttcgag tgacggtaac cgtattcctc ctacatctat tattgtttat gatatagaag
    16381 tttttgcaga ccataaataa aagggttcaa tttttctttt atttaaaagc aattttggaa
    16441 aagtcatgat atagatgatt cttctaaatt tgctcaaaaa aacaacatgc cccgttttaa
    16501 caaacttatc atctattatt tttccggaac aggtaattca aagaatgtag cgctttggtt
    16561 ttcaaaagtg gccacagaaa gaggtattat tacacttatt gtcaatattg cagaagtaga
    16621 tagattgcat atagaaacgc ctcctccaga tgcgttgatt atgtttgttt cgccaataca
    16681 tggatttaat tatcctccgg tgatgatgca ttttatcttg cgtttcccga aaggttttaa
    16741 caaggttgtg ctgatgaata cgcgcgccgg aatgcttatt ggcaaatggg taactcccgg
    16801 attatccggt ttggcttttt attttgcttc attggtattg aaattcaagc attattctat
    16861 tgttgggatg atgcctgtcg atctgccttc aaactggata tcgctgcatc ccggactgaa
    16921 tgagcgcacc gtaaaatttt tacacgagaa aaataaagtg agagtaaccg cttttgctgg
    16981 taaaattttg gccgggaaaa gatgttacaa agccttgaag gagattgtcc aggatttggc
    17041 aatatcgcca atttcattgt tgtactattt tgtggggcgt tttgttattt ccaaaacctt
    17101 ttatgcatct gcagactgca ataattgcga tttgtgtgtt aagaattgcc ctgtccgggg
    17161 aattatcaag gttaacaatc gtccattctg gacctatcgc tgcgaaagct gtatgaaatg
    17221 tatgagctat tgcccccaaa aagcaataga gactgctcat gcgccatttg cgatttttgt
    17281 tgttttatat aaccttgtgt tgatggtgct gttttacaag tattttgggc ttctgttctt
    17341 tacaatagag aatgtaattg tcaaattcgt tgttgaaaca gttatattta tagtttctct
    17401 ggctgtttgg taccggctta tgcatttttt gctgcgtttt cattggtttg aacgtttggt
    17461 ggtttataca tcgcttacaa aatatacctg gtggggtagg agatataaag cgttgaaaga
    17521 caattagcga attagttaat ttgaaaattt gaaaatttga aaatttgaaa ataaggtaat
    17581 gttaagggag caaacccata catattatga gcaaaaggct gaattgttgc ttcaatatat
    17641 tgccaaccat cttgatggcg atttgagtgt aaaaacattg tctgatattt ctgggatttc
    17701 gttgtttcat tttcacaggg tcatgaaagc tgcgctgggt gaaactttgg gaagttatat
    17761 tgaccgtgtc aggctcgata ctgctgttaa acttatcagg tatggcgata tttcttttac
    17821 agatatagct cttcgtattg gatttaagga tttatcagct ttttcaaaag cttttactaa
    17881 agagttcggt atttcgccta atgattttaa agcggataaa aatgctgtcc tcaatactca
    17941 cattgattat aaaatgacag gtacccaaaa actggttgta gatgttaagc cgaaaataaa
    18001 gaccctgcct gaccggatgg tttattatat cagggtgaag ggcatatatg gcggcttgga
    18061 agtaataaaa gcctgggatg aattgggaga ctttattgtc atgaataaac ttgacggctg
    18121 gaaccttaat ttgttttcgg tttattatga tgatggcgat gttgtgggtg ttgaaaatac
    18181 catttcagat gtttgcattg cttcaaagaa gatggtatct gtatcagggc gaatagccgt
    18241 tcagaacatc ccgggaggaa agtatgctgt ttataggtat aaaggcccat acgattattt
    18301 gtgggatgtg tatgatgcaa tctttcgtga tggggtatat ccgggcgatt ttaaactgcg
    18361 cgatgctcca attgtagaaa aatatctaaa cctttcgcac aaggtaaaac ctgttaattt
    18421 gttgaccgaa atttttatcc ctatagaatg aaatttgtac ttaaatattt caccggaacg
    18481 ggcaattcat tgaaaatact ggatacttgc aaagatgcct ttattgctaa gagccatgtt
    18541 gccagcatat cggcaattaa ggttggacag cagttgccgc aagatgccga tgtgattggc
    18601 ttttgctttc cggtctatgc tttcgggata ccccggattt gcaggcagta tttgaaggag
    18661 ctgaacaggt ttaagaaaca acagaaagta tttgttctca tctctgctgg tgatgcagat
    18721 gaggcgggtc ttgcatttcg caattgcgaa aaaattctta ctaagaagaa ttgccgggtt
    18781 atatattcgg cagtggttca aatgcctgtc aattggataa tttttgaaaa agcaccttct
    18841 gaaaaagaag cagaagctat tattcaaaaa ggggttgagt ttacatacca ggttgctcgc
    18901 gatattctga atggcgttca gaagtttcac cagtttaatt atccaaagcg ttacagtaag
    18961 tttgggtttt ataaagatta ctttttgttc aaatatttgg gcatttacaa tctttgtcgg
    19021 atgtttaaga cctatgacag ctgcaatgga tgccaattgt gctctaaaat ctgcccaact
    19081 caaagtattg tgatggtaaa taacaggcca acatggactt ataagtgcga acagtgtatg
    19141 cgttgtgtca atttctgtcc aaagggggct atatatcaaa tgtatggcgg tgacacactg
    19201 ggaaaggata gatattatga gccaagtttc aaaccattaa aggaaatgta attctaaaag
    19261 cgccaaacac agcctgctcc ctgaggcgta acatacaacg agtaattggc aggtttttcg
    19321 tcattcataa gttgtttaaa gctcaagcct actgtttttc cgatggcata gcccagaagg
    19381 gcccctgcaa atgcatctga gaaccagtga atgtttaccg aaacgcctaa cccaactatg
    19441 cttgcatagg ttattgcccc aattttcagg gctttgtttt cggggtaaag ttcggtaagc
    19501 gttatagcca tagcaaatgc tgtagtagtg tggccgcttg gccatccgtt gaatgctccc
    19561 ctgcgcaaga tgccaaatgc aaaatcgtcg ctgtaatctt ttgaacggtc agttatttct
    19621 tcatcatcgc gcaatattcc cggaggacgg cgcccggtaa agactttgat agaggtagta
    19681 agccctgcgc caagaattgc ggcttgacct aatgctaagc ctgtaatttg cagttttgta
    19741 ttgttatgtt tacgcccata aatgtaaacg ccaagaggga cagctaatgg aaccaatccg
    19801 ccaacagcaa cagcaggcat accgctatat gctacaaatt tattttcgta tgaaagtttg
    19861 tttatctccc agtcaatgcc gctttccacc aggccataag tgccggcaat ggcaacagcg
    19921 ctgtttaatc catagttgta ggtgaaggtg tgcaccgtat ttgccccaag tttgtaaaat
    19981 attttggaaa aagggatgat ggaacctatg tttgcaggct cgtatgaaaa gcttaattta
    20041 cttatcatta ataagcttaa agagaatata aaacgcattg ttgaaggggt aatcaaattt
    20101 ttgctccgaa gttaataatt atacgccgtc aaaccgtact atcttcagat atttttgcta
    20161 ccttagttag cgctaattgc cctgtgtatg gaaaaaacta ttgatcaatg gcaatttgaa
    20221 caaagtacta ccgattttaa ccggttgatg caaaaacgca tttacagggt tttgctggtc
    20281 tgtagccggt atgattattt tattcttgaa gaagatggtc gcattgagga gcaaatcttc
    20341 aatgaataca tggctttgaa tatgcgctat cctccggcgg taattcagac tgactcagca
    20401 gaagaggcgc tcaaaatcct tgaagatgaa gagacagatt tggttatatt aatgctgaat
    20461 attggcgaat ctgagccatt cagtttagcc aggaagataa aagacctata tcctggcaaa
    20521 cctattgtgt tgcttacgcc atttttgcgt gaagtttctg cccatttgaa acatgaggat
    20581 ttgtcagcta ttgattatac attctgttgg cttggtaatg cagatttgct ggtagctatt
    20641 attaagctca tagaagacaa gatgaacgct gctaacgata ttattaacaa cggcgttcag
    20701 gcattgttgc ttgttgaaga ttcggttcgt tatatatcgc annnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnnnn nnnnnnnnnn nctttgtaaa tatatacggg aaaaagaccc atttttggct
    20881 tttctttttc aatcgtcaga taagtcaaat gcagaacgag caaggcagtt gaatgcaggt
    20941 tttttgtgga aacagtcaaa aagcctttcg cacgagttga aacagtacat tatcgagaat
    21001 tttgggtttg gcgaccttgt attttctaat cctaaaggga aaggagagac aattcgtatt
    21061 catgatttgg cagatttgca gaagtttata tttgacatct ctgatgaagc ttttatttat
    21121 catgcccgtc gtaatgaaat ttcgcgttgg ctcaatgccc gtgccttgtt tagtataggc
    21181 agcttgttca aggatttgca aatagaagat ttcgaaacat tgaatcaggc tcgttcctgt
    21241 atttttgatg ctattgccat ataccggata accaattgca aaggggtaat tgccagtttt
    21301 aataaaaacg attttaatga atacctgact ttttgccgga taggagaggg ttctcttgga
    21361 gggaaaggac gaggactggc ttttcttgat gcgcttatcc gaaggcataa tctttatgat
    21421 aagtttgaaa agacgcatat tgttattccg cgtacagttg ttatagctac agatctgttt
    21481 gacgaattta tggagcagaa cgacctgtac aaaattgcct cctctgaaag cgatgataaa
    21541 aagattttag aaaagttttt aaaagcatcg ctgcccaatc ggttgtatca cgacctacag
    21601 aattatgtaa agaaaattca gaaccctgtg gctgtccgtt cttcgagcaa gcttgaagat
    21661 tcccatttta tgccgtttgc cggggtgtat cgcacatata tggtgcctta ttcaaaggat
    21721 gaaaaaaaga tggccgagat gctctgcgat gccattaagt gtgtttatgc atctgtttat
    21781 tataaggaga gcaaggcata tatggcttct acatcttcgg tgattgacga agaaaagatg
    21841 gctatcattc tggaagaagt ttgtggaaag gcttatcagg accgttttta tccggctatg
    21901 agtggtgtag cgcgttcggt gaattattat cctgttttgc cagagacagc aggtgatggc
    21961 gtagtaaaca ttgcttatgg actggggaaa tatttggttg acggaggaag tgggatgcgt
    22021 ttttgtccaa aatatcccaa gaaagctttg cagctttcgg tgccggaatt ggc
//
LOCUS       DAOSPF010000060        20493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2602_length_20493_cov_7.113220, whole genome shotgun sequence.
ACCESSION   DAOSPF010000060 DAOSPF010000000
VERSION     DAOSPF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_20493_cov_7.113220"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PK252_11205"
     CDS             complement(<1..1015)
                     /locus_tag="PK252_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT15317.1"
                     /translation="MSYLFEITFLLEQFLKGTWTKAPLGVWDKLKRLSGVFLLLFLLL
                     ICSSYAQAQSLEDENDELSTEQSISEGESDETVIDNIERTEIYESLINQPLDINTATA
                     DDFAKIPFLNSMQIDEIIQYREKNGSFVSVNELNAITSLSEEEVRNLIPYCTANPITK
                     EEQKPFRAKHVFESVVGTMFPKMEGFKAIDDSSSSNKFLCLVPLKSRVRYSFMGINSE
                     IRFLAENDYGEPYFNSGNKAFDYLSAGYFYHSKNWLKALTIGDYTARFGQGLAMWNGY
                     ASSFSTTSEGAFKDRSGLAGYSSFNESQYLRGVGTTFKVKNIGITAFGSYKAIDASII
                     LTDS"
     gene            complement(1137..1952)
                     /locus_tag="PK252_11210"
     CDS             complement(1137..1952)
                     /locus_tag="PK252_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15318.1"
                     /translation="MNKTLKALLCLVFFISVSAKAQQESAGARLMAMGNCGTAVYDHW
                     SSAYNQAGVAFAEKANVNTFYQRRFNIKELSTSAFSAIMPTRLINIATDLKYFGYSKY
                     NKTLCGVSFARLFGQRFAAGLRCSYLQEHIEGNSNKNRLFNLDCGVMANLQPFVIGCK
                     VQNIIPPDTYEYKYPEYETNGAVGVAWQPLSLFIFSAEIESSLKSKILYRSGLEYTAW
                     QILQLRTGIIKQNDQHAFTFGIGLLLKRFHVDIAMSKYQYLPFSQAFEAGVSF"
     gene            complement(2051..2839)
                     /locus_tag="PK252_11215"
     CDS             complement(2051..2839)
                     /locus_tag="PK252_11215"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HOT15319.1"
                     /translation="MRSIYLFVGLSIVFLSFGFVRTDDSTQKTVVKNIPLGKAEIVET
                     EIEFVVGRLNISSSATQLTEGTYKFYKEILKPEFSYTEEGKTGHLSITSKKGHIQMDD
                     DNDNNNDNECLWDIALNKKVKHDLTVKLTAGNGNIDLSGCQLKRFEFKMVAGEAKINL
                     SNTSVPEVNFKAMAGNATIDLSGKWNNDLHARIKGGVGEITLKLPADIGIRMNISGVL
                     GSVNTKGLYKMGQEYTNELLGKTKQTIYIDVTGGIGNVNVKLVE"
     gene            3065..3391
                     /locus_tag="PK252_11220"
     CDS             3065..3391
                     /locus_tag="PK252_11220"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT15320.1"
                     /translation="MTDLFVTKNNHWALFMGHLVIEKLLKALYVKNINDFPPLIHDLR
                     RIIEKSGVELSIDKQIILDSISRFNINARYDDYKQSFYHLCTDSFTSEWIEKIKDCRL
                     WIKTML"
     gene            3370..3684
                     /locus_tag="PK252_11225"
     CDS             3370..3684
                     /locus_tag="PK252_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT15321.1"
                     /translation="MDKNDAIRISKGYLQKLRNANLGFAEAYLFGSFAKGNQHDNSDI
                     DIAIVLKDNVNHTFDTDVQLMVIRKGEETIIEPHAFSKEEFDYQVPIVNQILKYGIKI
                     EI"
     gene            3945..5450
                     /gene="amrB"
                     /locus_tag="PK252_11230"
     CDS             3945..5450
                     /gene="amrB"
                     /locus_tag="PK252_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT15322.1"
                     /translation="MAKKRRICTLWIYVVMVGILGNCNGQTPGNTMKKIDRQPAVAGT
                     FYPADADGLRSMLDSMFKGLKPETKEQPLAVIVPHAGYVFSAGVAAKAISQIDREASF
                     DRIFIIGSSHTMHFDGSSVYTQGDFITPFGKVEVDALAEQLCEKHSFISSNPQPHARE
                     HCLEVELPLLQYWLKKPFKIVPIVIGGYTEKTSKKLAEALEPYFNSNNLFVISTDFSH
                     YPKYADAVMSDGIMAEAIVSNSSRKFLLEKEALESKGISNLATAVCGWTSVLTLLYIT
                     EKLEGITIKKIDYKNSGDSPYGGNDKVVGYNAICVYQENKQPSPLSDSDKKKLLTLAR
                     KTITDYANKIEIKDPDAKDFEGGASMLAGAFVTLHKNGKLRGCIGTFRADQPLYKTVQ
                     SIAISSAFADSRFEPVSAEEVPSLHIEISVLTPMRKINSIDEIVLGKHGIYIRKGSSS
                     GVFLPQVATEQKWTKEQFLGYCARDKAYIGWDGWRDAEIYVFESIIFEEAK"
     gene            5498..6568
                     /gene="amrS"
                     /locus_tag="PK252_11235"
     CDS             5498..6568
                     /gene="amrS"
                     /locus_tag="PK252_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT15323.1"
                     /translation="MGSLIYAGSAAGMFAGKDKPWRWAHEAMFYSVTPHGVRCGLCPN
                     QCLLGEGVTGKCRNRINYEGKLFSIAYGNPCAVHIDPIEKKPLYHFLPNSRAFSIATA
                     GCNFACLNCLNWQISQVGPRETSNEDLMPDEVITECLAGKCEAIAYTYSEPVTFYEYV
                     FETAKLAKAKGIKNVFISNGYINPGPLKKLIPYLDAANINLKSFSDDIYQRLNGGTLQ
                     PVLNTIKTLKDNNVWVEVTNLVIPDWTDNFDMMREMCRWLVDNGFENYPLHFNRFFPQ
                     YKLLKTPPTPIPTLVRAKQIAQEAGMKYVYLGNNPEGSDNTVCPKCKKVVVERTGFST
                     TAVHIKAGNCKYCGEKINGVWS"
     gene            complement(6617..6691)
                     /locus_tag="PK252_11240"
     tRNA            complement(6617..6691)
                     /locus_tag="PK252_11240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6654..6656),aa:Val,seq:gac)
     gene            6804..7577
                     /locus_tag="PK252_11245"
     CDS             6804..7577
                     /locus_tag="PK252_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT15324.1"
                     /translation="MNTKIISYNVNGLRAALSKEFALWLQSEAPDIICLQETKLQQGQ
                     VDTTIFEQMGYYHYWHYAQKKGYSGVALFTKKKPLNVFYGIGKEPYDSEGRLVAAEYD
                     GYSAISVYFPSGTTGDIRQEIKMAFLQDFLAFIENYKNQQPNLIISGDFNICHKPIDI
                     NHPERHEGYSGFLPEERAWMDSFQAAGYVDSFRVFNGKPNHYSWWSYRAGARSKNLGW
                     RIDYHWVSEPLKEKLVSAQILSSVIHSDHCPVEVVIDLP"
     gene            7666..8562
                     /locus_tag="PK252_11250"
     CDS             7666..8562
                     /locus_tag="PK252_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT15325.1"
                     /translation="MTTRIKKSLTIALLAFAAIIGNAQTAYFEMNRIQACDSFYVRCY
                     NKCTASVGSSVDNDYTYTWNIEGNTTIIKKNTSPIRVWLKKPGLNKITLTMAHNTHSG
                     EKASYTNSFLVRPKPNAWFTVADTFALGNLTYRIRTGKVPADSIKYIYRYTLDGQLLA
                     LHDSAKEKRDTTFMRFSSPGEHTVALTVKDWYGCTARHDTVFYASDKLIVPKVFTPNG
                     DNINDYLFVETNGRDIYHFEVFNSNGLRVFHSESPSIMWDGIIEANGQEAPAGTYYYI
                     LEPKSAGGKAKTKGYFVLLRKK"
     gene            8564..9073
                     /locus_tag="PK252_11255"
     CDS             8564..9073
                     /locus_tag="PK252_11255"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT15326.1"
                     /translation="MNNQKPLSIIVAVAKNYAIGYQNKLLWHIPEDFRWFKSITQGHT
                     VIMGKNTYYSLPKRPLPNRRNIVISDIRGEFIEGAEMAYSIDEAIAMADKDKENFIIG
                     GASIYRQFFDIVQKLYITHVDKEFVADTFFPVITADKWQVEKEAQQQEEHPQGLKYHF
                     VIYKKSNSF"
     gene            9135..9986
                     /locus_tag="PK252_11260"
     CDS             9135..9986
                     /locus_tag="PK252_11260"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOT15327.1"
                     /translation="MPTFVGYNIFSKDKTKLHFKIWDVAQPKICICIVHGLGEHSGRY
                     EEWANMFGEKNIAVASFDFRGHGLSDGKRGHAFSFHKLYDDIDAFLTEVKRRFPLVPL
                     VLYGHSLGGSLVLNYYMSGKSYHTSAIIVTSPWFKLVNEPSKFLIDFTMALSRFLPFI
                     TVSNRINPLLLSRDRQIAEEYTKDPLVHNKISLRLFHEAYRMGIKASKQAYKLNIPTL
                     IMHGENDAITSSATSVEFARNTGPFLSLHDLCKKMVFQHILHWLQVNNLYREDNSPKN
                     VVIQNYY"
     gene            9961..10941
                     /locus_tag="PK252_11265"
     CDS             9961..10941
                     /locus_tag="PK252_11265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOT15328.1"
                     /translation="MLSFRTIIEINPSDSKISHNTPMCMMGSCFATNIGEKLESLKFD
                     ILLNPFGILFNPLSIAASLKRTVENNPFTEQELVFQHEMWHSLAHHGKFSHTDKSKCL
                     ENINSSLAEAHEMILKSKFLIMTFGTAWIYFYKKTGNVVANCHKLPSSAFDRRLVSVE
                     EVYTNVFDSLSLIKKINPGIQFIFTVSPVRHLADGAHANQLSKATLLLAVSQLCNTFE
                     AQYFPAYEIILDELRDYRFFADDMAHPSPLAVDYVFERFAETYFNKETRHLNLKIDEI
                     YKAVNHRPVNENSIAHEKFKEIMLKKIEDIEKANPYLDFSEEKKHFILNR"
     gene            complement(11127..13565)
                     /locus_tag="PK252_11270"
     CDS             complement(11127..13565)
                     /locus_tag="PK252_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOT15329.1"
                     /translation="MKYGFFDDKAREYVITNPRTPWPWINYLGNEDFFSLISNTAGGY
                     TFYKDAKFRRLTRYRYNNVPMDSGGKYFYIKDGDTIWSPGWKPCKTELDNYECRHGMN
                     YTNIKGVKNGVTANVLYFVPLKTWAEVQKLTLTNNSKETKKLKVFSFVEWCLWNAAAD
                     MENFQRNFSTGEVEIEGSVIYHKTEYKERRDHYAYYSVNAPIQGFDTDRESFFGLYNG
                     FDEPQVVAEGKPRNTEAHGWSPIASHYLEVELKPGESKDFVFVLGYVENKEDEKWESK
                     NIINKKKAKETIAKFDTPEKVDAALAELRKYWDNLLSVYNVVSGDEKLDRMVNIWNQY
                     QCMITFCFSRSASYFESGIGRGMGFRDSNQDLIGFVHQIPERARERIIDIASTQFPDG
                     GCYHQYQPLTKRGNNDIGGGFNDDPMWLILGTISYIKESGDFSILDEMVPFDNDMSVA
                     RTMFDHLTVSFDHVVNNLGPHGLPLIGRADWNDCLNLNCFSNDPNESFQTTENKTEGS
                     KAESVMIAGLFVVYGREYVELCKKIGKNDEAARAQKEVDNMVAAIKKHGWDGEWFLRA
                     YDYYGRKVGSNENEEGKIFIESNGWCTMAGIGKEEGLCTKALDAVKERLDCKYGIVLN
                     NPAFTKYYIEYGEISSYPAGYKENAGIFCHNNPWIIIGETVNGNGERAWEYYKKICPS
                     YLEDISDLHKTEPYVYAQMIAGKDAFRPGEAKNSWLTGCAAWTFTSITQYILGVRADY
                     DGLIVDPCIPSSWEGFTVTRKFRGAIYVIKVSNPDKVQKGVKRLTVDGKEISGNMIPL
                     QPAGKTYNVEVVLG"
     gene            complement(13598..15310)
                     /gene="recJ"
                     /locus_tag="PK252_11275"
     CDS             complement(13598..15310)
                     /gene="recJ"
                     /locus_tag="PK252_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT15330.1"
                     /translation="MEKNWIIHTPGKTEEVEHLMQVLGVEYPIANLLVQRGINTFEEA
                     KMFFRPELENLHDPFLMKDMGKAVERLQAAIDNGEKVMVYGDYDVDGTTSVAMMYTFL
                     KKYIKKLSYYIPDRYTEGYGISIKSIDLAASDGVTLVIALDCGIKAVEKIAYAKSLGI
                     DFIICDHHTAGAAIPEAYAVLDPKRPDCSYPFKELSGCGVGFKLLQAFCMKNSYSFEP
                     LYEMLDLVVVSIASDIVPITGENRILAYFGLKQLNSAPRSGLKAIIQIAGIENKEICI
                     DDIVFKIGPRINAAGRIETGTSSVELLIAENEALALDMGDMINDFNNTRRNIDSNITK
                     EALDMIASDKDQHNRKTTVLFNPSWHKGVVGIVASRLIETYYRPTIVLTESNGLITGS
                     ARSVTGFDLYKAIEGCSHLLENFGGHMYAAGLTMKKENFKAFRIKFEEVVAESVMNEQ
                     LIPCIEVDMELKLKDITPKLYRIIKQFEPFGPNNMAPVFFTENVVDDGTGRIVGSNKE
                     HLKLNIIHEDEPFKPYPAIGFNLSNHYPYISKGYSFDICYCIEENEYRGQTSIQLKIK
                     DIKK"
     gene            15640..17106
                     /locus_tag="PK252_11280"
     CDS             15640..17106
                     /locus_tag="PK252_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT15331.1"
                     /translation="MMKNILFAFCIIIALIATSCKKDKADSATISELDISINQFIWDI
                     MSTNYLWADNVPALNSSNYSSTIDIEEKLLKKYSDHKKFFDTLLYYKGSVDKYSFIID
                     DYEKFEASRQGYAKSMGFYYNLYLFNNYKNDDRLFGLVMYVVKGGPADKAGIKRGDFF
                     FIIDDTQLTINNYQNLLSKESYKISLAYYHAYNKEITVTGTSCNLTAEALQENPIFLD
                     TIYSINSRKIGYLIYNYFDPNFDSRLNAIFAKFKQEKISDLILDLRYNGGGAYSSAIN
                     LASMIYSTDNTKVFVKNEYNQKLQQKLLEKYGEGFFKACFTSKIIKGDAITEENINSL
                     GLSKIYIITTQSTASTSEMLINNLMPYIKVYTFGNKTFGYGVGLMYFKDYDQLNRLNT
                     RHKYAMQIVVLKTLNVNGNGFNTVGIEPYIAVDENYTSLLPLGDRNETLLNEVISNIF
                     SSKSAKLNNPTIFDFYADKNDLIPHKKEIFMNTISFKK"
     gene            complement(17459..18442)
                     /locus_tag="PK252_11285"
     CDS             complement(17459..18442)
                     /locus_tag="PK252_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15332.1"
                     /translation="MIKKLLYFSFAVFFTACSDDDPEKTKVVLSNLDNKEITSIVLDN
                     FNKLWVGTDSGLYCQVEAGYELQDVVAATKILSLGYEKASNTLWVGTSNGLTKCTITD
                     GVLSGTGIDAAKLSSNKVYASHIDAKSIRWFGTASGPTMNKDDKWKKENLLVNALDEY
                     INLEFAKSQVNAIASWDGDVYFATSGKIIYRAYGYNESLDAFTGASQLSSPYNGPCLS
                     DTMNAVFVDSQNRIWMGGNSGLQFIVGHETKSPDIFGFTENDLPSARIHAIAEGKDGR
                     LWVGTEKGLAILENQTWTAKTSGLPNLYVTAIAFDKDGKAWVGTKKGLVKL"
     gene            complement(18494..>20493)
                     /locus_tag="PK252_11290"
     CDS             complement(18494..>20493)
                     /locus_tag="PK252_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT15333.1"
                     /translation="STIWFLVRVIPKPQRAAYPCMRTAAPLMSGFVIYLLSLGGSLLF
                     FRKAIANLKKAKYWAAIGAIAISFVLVVIFNINDVKQIYANTLGIQWTRGVLPDAPNS
                     PMGTAWGIKPGRVAWAWNPASTRDECTNMIVSTGGWNGVMDLTKSDAYFQAKNTNQDT
                     INMLANKAIMLIGGESTVKASWDAIFKSFNKKKTGTESSYVAGQKIFIKVNNGQAGWA
                     IYSDLSEKGNSGQGVGNAMSNTSPQAVLAFLVQLIDSCGVAQSDIIIGEPMSHVYKSM
                     ADIVWPMYPKVIIIDKDDKTSLGRTTSSGWVSNAIVWSDKGKVMPSAINDDMMQEMYD
                     ADYLINISALKAHARSGVSLSAKNHFGSSSHGGSYRAERLHAGSIATGDMKTGNDDLS
                     AAHCRGDYHMYRVLTDIMGHEKLGGNTVLFVVDGLWSGIEATDMPVKWGIAPFNNDWP
                     SSLFVSQDEVALESVCLDFLRAEADKNTKFKDRPFFPAVDDFLHQAADPANWPTTVKD
                     IDGKSYPFAGYDPEGDGTYMKSLGVHEHWDNPINKQYSRNISADGKGIELVSYPSGLV
                     SNKTAIKVSQVEASLNAYPNPVVNSCFITYQLKASASVSLFIASTDGRMIEYIEQKQL
                     AAGSYTKTFDASKLKSGAYVCVLKARYNGRSEIKSLKLQVR"
BASE COUNT         6189 a         4121 c         3952 g         6231 t
ORIGIN      
        1 gtgaatcagt aagaattatg ctggcatcaa tagctttgta gctcccaaat gcagtgatgc
       61 caatgttttt aactttaaat gttgttccta ctcctctcaa atattgactt tcattaaacg
      121 aagaatatcc tgccaggccg cttctgtctt taaaagcacc ttcggaagtt gtgctgaaag
      181 acgaagcgta tccgttccac attgccaacc cttgcccaaa ccgggctgta taatcgccta
      241 tggtcaaagc tttcagccag ttctttgaat ggtagaaata tccggcagaa agataatcaa
      301 aagctttgtt cccgctgtta aaataaggct cgccgtaatc attttcggcc aaaaacctta
      361 tttcactgtt tattcccata aatgaatatc gaacccttga ctttaaaggt actagacaga
      421 gaaatttatt tgaactacta ctatcatcaa ttgctttaaa gccttccatt ttaggaaaca
      481 tcgttccgac aacactttca aatacatgtt ttgcccggaa aggcttctgt tcttcttttg
      541 taataggatt agctgtgcaa tatgggatta aattacgtac ttcttcctca gaaagagatg
      601 tgatagcatt aagctcattg accgacacaa aacttccatt tttttcgcgg tattgaataa
      661 tttcatctat ttgcatacta ttcagaaagg gtattttagc aaagtcatcg gctgttgccg
      721 tatttatatc aagtggttgg tttatcaggc tttcataaat ctctgttctt tcgatgttat
      781 caataaccgt ttcgtccgat tctccttctg aaatggactg ttctgtactt agctcatcgt
      841 tttcgtcttc taaggactgg gcttgagcgt aagaggagca gatgagcagg aggaatagca
      901 gcaagaagac cccacttaac ctcttcaatt tatcccaaac acctaaaggg gctttagtcc
      961 aagtcccctt cagaaattgt tcaagtaaaa aggttatttc aaataaatag ctcataatcc
     1021 cattttttga taggtcaacg cactgttccc ctttcatata aaggagggcg tgtcccgcct
     1081 caggcgggag tggttttatt atatttgaac aaagcaacat acggtgcatg cagattttaa
     1141 aagcttaccc ctgcttcaaa tgcttgtgaa aatggcaagt attggtattt gctcatggca
     1201 atgtcaacgt ggaagcgctt caacaacaat ccaatgccga aagtaaaggc atgttggtcg
     1261 ttttgcttta taatgcccgt acggagctgt aaaatctgcc atgcagtgta ttctaaccct
     1321 gaacggtata aaattttcga cttgagactc gattctatct cagcactaaa aatgaatagg
     1381 cttaatggtt gccaggctac gccaacagcg ccattggttt catattccgg gtatttatat
     1441 tcataagtat ctggtggaat aatattttgc actttgcatc caatgacaaa tggctgcaaa
     1501 tttgccatta cgccacaatc taagttaaat agtctgtttt tgttactatt tccttcaata
     1561 tgttcttgta ggtagctgca acgtaagccg gcagcaaacc tttgcccgaa taacctggca
     1621 aaacttacgc cacacaaggt tttgttatat tttgaatagc caaaatattt caaatctgtg
     1681 gcaatgttga taagcctggt tggcatgatg gccgaaaaag cgctggtaga aagttctttg
     1741 atgttaaaac gcctttgata aaaggtattt acatttgctt tttcggcaaa agctacacct
     1801 gcctggttat aggcagaaga ccaatggtcg tacacagcag tgccgcaatt gcccatggcc
     1861 atcaatcggg ccccggcact ttcttgttgg gctttagcag atactgagat aaaaaaaaca
     1921 aggcatagca atgcctttaa cgtcttattc atacaggtta acggtttggt tgggttctta
     1981 aagatagaaa aataatttat atttttacct gcaacctgta aaacctgata tgatatctat
     2041 tgatttttaa ttattccacc agcttcacat taacatttcc aatgccacca gttacgtcaa
     2101 tataaattgt ttgcttggtt ttgcccagca gttcgttggt gtattcctgt cccattttgt
     2161 aaaggccttt agtattgaca gaaccgagta caccgctgat gttcattcta ataccaatat
     2221 ctgcgggtag tttcaacgtt atttcaccta caccgccttt aatacgggca tgaaggtcgt
     2281 tgttccattt accactaagg tcaatagtgg catttccggc catagcctta aagttcactt
     2341 ctggtaccga ggtattgctg aggtttattt tcgcttcacc agccaccatc ttgaattcaa
     2401 accttttgag ctgacaacca cttaggtcaa tattgccatt gcctgctgta agcttgacag
     2461 tcaggtcatg ttttactttt ttgttaaggg caatgtccca caaacattcg ttgtcgttgt
     2521 tgttgtcgtt gtcatcgtcc atttgtatat gccctttttt tgacgtgatg ctgagatggc
     2581 cggttttccc ttcttcagta taagaaaatt cgggtttcaa tatttcttta taaaacttat
     2641 aagttccttc agttagttgg gttgcagaag aactaatatt caacctgcct acaacaaact
     2701 ctatttctgt ttcaactatt tcggctttcc caagcggaat gttttttacc actgtttttt
     2761 gggtcgaatc atctgtacgg acaaagccaa aagataagaa cacgattgac aagccgacaa
     2821 aaaggtatat acttctcatg tttttagttt ttaaagtaaa taatctgtag gacgatttgg
     2881 ggcactaaaa gttacaacaa ctgtttcaga tatccaattg ttgacatgta ttaataatca
     2941 tagtttcaaa aatgaattat ctttgtatca ataagtaaca cccaacatat cagtggataa
     3001 aggatttgat aaagaacgaa taattgctca ttggattgaa agttatgaca aggatttcta
     3061 aactatgacg gatttatttg ttactaagaa caatcattgg gctttattta tggggcatct
     3121 ggtaattgaa aaattactta aggcattgta tgtcaagaat ataaatgatt ttccgccttt
     3181 aattcatgat ttacggagaa ttattgaaaa atctggagtt gagttaagta tagataaaca
     3241 gataattttg gatagtattt ccaggttcaa tattaacgcc aggtacgatg actataagca
     3301 aagcttttac catttgtgta ccgacagctt tacttctgaa tggattgaaa agattaagga
     3361 ttgcagatta tggataaaaa cgatgctata aggataagta aaggatattt gcaaaagctc
     3421 agaaatgcta atttaggatt tgctgaagca tacttatttg gctcatttgc caaaggcaat
     3481 caacatgaca atagtgatat tgatattgcg attgtgttaa aagataacgt aaaccacaca
     3541 tttgatactg acgttcaatt gatggttatt agaaaaggcg aagaaacaat aattgaacct
     3601 catgcttttt caaaagaaga atttgattac caggttccta ttgtaaatca aattttaaaa
     3661 tacggaataa aaatagaaat ttaacacttg atatcccttg gtttaagcaa tgacaggtgt
     3721 tataaagtat tatacatctt gccgttcgtt ctgttttggc aaaaatgacg caactaaact
     3781 tgaaacgata gtcaatgctt gcatttttaa ttgcagtggc ttagccccct ttccttttag
     3841 gaaagggttg gggataggtc actgaatatg gttttttaga aataccaagt tgataaattg
     3901 caatctgccc aaatctgaag taactttggg taaacccaag caacatggca aagaaaagac
     3961 gaatttgcac attatggatt tacgtggtga tggtgggtat tttgggaaat tgtaatggtc
     4021 aaactccagg taataccatg aaaaaaattg acagacaacc agcagtggca ggtactttct
     4081 accctgctga tgctgatgga cttcgcagca tgttggacag catgttcaaa gggctgaaac
     4141 ctgaaacaaa ggaacagcct ttggcagtaa ttgttccgca tgccgggtat gtattttcgg
     4201 caggagtggc agctaaagcc atttcgcaga ttgaccgtga ggcaagtttt gataggattt
     4261 tcattattgg ttcgagccat accatgcatt ttgatggctc ttcggtttac acccaaggcg
     4321 attttattac accttttgga aaagtagaag tagatgcttt ggccgaacag ctgtgtgaaa
     4381 agcacagctt tatttccagc aacccgcaac cccatgccag ggagcattgc ctggaagtag
     4441 agttacctct tctgcaatac tggctaaaaa agccttttaa gattgtccct attgtaattg
     4501 gtggatatac cgaaaaaacc agtaaaaaat tggctgaagc ccttgaacca tattttaact
     4561 cgaacaactt gtttgtgata agcactgatt tttcgcacta tccaaaatat gctgatgctg
     4621 taatgtctga tggtatcatg gctgaagcca ttgtttccaa ttcatcccgc aaatttttac
     4681 ttgaaaaaga agcgcttgaa agcaaaggca tttctaacct tgctacggct gtctgtggct
     4741 ggacttcagt ccttaccctt ttgtacataa cagaaaagct cgaaggcatt acgattaaaa
     4801 agattgatta taaaaattcc ggcgattcac cttacggagg aaacgataag gtggttggat
     4861 ataatgctat ttgtgtatat caggaaaaca aacaaccgtc tcccctctcc gattcggata
     4921 aaaaaaagct gctaaccttg gcccgtaaga ccattactga ttatgcaaat aaaatagaaa
     4981 ttaaagaccc tgatgctaaa gactttgaag ggggcgcatc tatgttggcc ggcgcttttg
     5041 ttaccttaca caaaaatgga aaattgaggg gctgcattgg aacatttcgg gctgaccaac
     5101 ctttgtacaa aacagtgcag tcaatagcta tatcatctgc ttttgctgac tcgcgctttg
     5161 agcctgttag tgcagaagaa gtgccttcgc tacacattga aatctcggtg ttaacgccta
     5221 tgcgaaagat taattccatt gacgaaattg tactgggcaa gcatggcatt tacatccgca
     5281 aaggcagcag ttcgggggtg tttctgcccc aggtggccac tgagcaaaag tggacgaaag
     5341 aacaattcct gggctattgc gcccgtgaca aggcgtacat tggatgggac ggctggcgtg
     5401 atgctgaaat ctatgttttt gaatcaatca tttttgaaga agcgaaatga atggcaatac
     5461 gatatcgaaa cgtgaattta ttaaacgaat agtagcaatg ggaagcttaa tatatgccgg
     5521 ctcggctgca ggcatgtttg caggcaaaga taagccctgg cgttgggcac acgaggctat
     5581 gttttattct gtaacacctc atggcgtaag gtgtggactg tgcccaaacc aatgtttact
     5641 gggcgaaggt gttacaggca aatgccgcaa ccgcatcaat tatgaaggaa agttgttctc
     5701 catagcatat ggcaatccct gtgcggtaca tatcgacccc attgagaaaa agcctttgta
     5761 ccactttctt cccaatagca gggccttctc tattgccact gcagggtgca attttgcttg
     5821 cctcaactgt ctcaactggc agatttcgca agtagggcct cgcgaaacca gcaacgaaga
     5881 cctgatgcct gatgaggtta ttaccgaatg tcttgctggc aaatgcgaag cgattgccta
     5941 tacatattct gagccggtca ctttctacga atatgttttc gaaacagcta aactggccaa
     6001 agccaaaggg attaagaatg tgtttatctc taatggctat atcaatcccg gcccgctgaa
     6061 gaagcttatc ccttacctcg atgcagccaa catcaacctg aagtcgttta gcgacgatat
     6121 ttaccaacgg cttaatggcg ggacgcttca gcctgttttg aacaccataa aaaccctgaa
     6181 agacaataat gtgtgggttg aggtaaccaa cctcgttatc ccggactgga cagacaattt
     6241 cgacatgatg cgcgaaatgt gccgttggct ggtcgataat ggttttgaaa attacccact
     6301 gcatttcaac cggttttttc cacaatataa actgctgaaa actccgccaa cacctattcc
     6361 tacattggtg agggcaaaac aaattgcgca ggaagcagga atgaaatatg tttacttagg
     6421 caataatccc gaaggctcag ataatactgt atgccccaaa tgcaaaaagg tggtggtcga
     6481 acgtacaggc ttttcaacca cagcagtgca tatcaaggcc ggaaactgta agtattgtgg
     6541 cgaaaagatt aacggggtgt ggagttaagc aaaacaaaca aaacaagtgg ttgtcctaaa
     6601 ttgtcaatta aaaatgtggg ttgtactgga ttcgaaccag tgacccctac cctgtcaagg
     6661 taatgctcta aaccaactga gctaacaacc cgtatttgtt ataaaaacaa ttctataatg
     6721 acaaaattca tctgcctaca tattgaaggc aaaagtaaaa aaagttttta tttttacatg
     6781 cttcccataa aataagctta tctatgaaca caaaaattat atcatataat gtaaacgggc
     6841 ttagggctgc cttgtccaaa gaatttgctt tatggcttca gtccgaagcc cctgacatta
     6901 tttgcttaca agaaactaag ttacaacaag gacaggttga tacaacaatt tttgaacaaa
     6961 tgggttatta tcactattgg cattatgcgc aaaaaaaagg ctatagtggc gttgctttat
     7021 ttacaaagaa aaaaccgtta aatgtttttt atggcattgg caaagaacct tatgatagcg
     7081 aagggcgcct tgttgcagcc gagtatgatg gttacagcgc catatcggtg tatttcccat
     7141 cagggactac cggagatatt cgtcaggaaa taaaaatggc tttcttgcaa gattttctgg
     7201 catttataga aaattacaaa aaccaacagc caaatctgat tatctcgggc gactttaaca
     7261 tttgccacaa gcccattgat atcaaccatc ccgaaaggca tgaagggtat tcggggtttt
     7321 tgccggaaga aagggcttgg atggactcat tccaagcggc tggctatgtt gattctttca
     7381 gggttttcaa tggaaagccc aaccattaca gttggtggag ttacagggca ggggcacgca
     7441 gcaaaaacct tggctggagg atagattatc actgggtttc tgaaccactt aaagaaaaac
     7501 ttgtatctgc acaaatattg tcctcagtaa tacattcaga ccattgcccg gttgaggtgg
     7561 ttatcgattt gccttgaaat taacactttg cgaaataaaa tatggcttag taatttttta
     7621 tactgcattt ctgcgtatat ttgaaacgaa taaattattt gccgcatgac aacacggata
     7681 aaaaaaagct taactattgc cctactggct tttgcagcaa taattggcaa tgcccagaca
     7741 gcatattttg aaatgaaccg catacaagcc tgcgattcat tttacgtcag gtgttacaat
     7801 aagtgtacag cttctgttgg aagttcggtg gacaatgatt acacttacac ctggaatatt
     7861 gaagggaata caacaattat taaaaaaaac acatcgccta tcagggtatg gcttaaaaag
     7921 cctggattaa ataaaataac ccttaccatg gcccacaata cccatagtgg cgaaaaggct
     7981 tcttatacaa actcttttct ggtaaggccc aaaccaaacg cttggtttac tgtagcagat
     8041 acttttgctt taggtaacct tacctacagg atacgaacag gaaaagtgcc tgcagattct
     8101 attaagtata tttaccgtta cacattagat ggccaattgc tggctttgca tgattctgca
     8161 aaagaaaaac gcgatacaac ctttatgaga tttagttcac ccggcgaaca taccgttgct
     8221 cttactgtaa aagattggta tggatgtaca gcccggcatg atacggtatt ttatgcttca
     8281 gacaaattaa tagtgccaaa agtttttacc cccaatggcg acaatattaa tgattatctt
     8341 tttgtagaaa ccaatggacg cgacatttat cactttgaag tgtttaactc aaacggactt
     8401 agggtttttc attccgagtc tccttcaatt atgtgggatg gaatcatcga agccaatggg
     8461 caggaagctc cagccgggac atattattac attttagaac ccaaaagtgc aggaggcaaa
     8521 gcaaaaacca aaggatattt tgttttattg cggaaaaaat aacatgaata accaaaaacc
     8581 tttgtcaata atagtagccg ttgcaaaaaa ctacgcaata ggctaccaaa ataagctttt
     8641 gtggcacata cccgaagatt ttcgttggtt taaaagcatt acacaagggc acactgttat
     8701 catgggcaaa aacacttatt attcgttgcc caaacgtcct ttgcccaaca ggcgcaacat
     8761 agtcatttct gatatccgcg gtgaatttat tgaaggtgct gaaatggcat attctattga
     8821 tgaagctatt gctatggccg acaaggataa agaaaacttc attattggcg gggcttctat
     8881 ttaccgtcaa ttttttgata tagttcaaaa gctttatatt acccatgttg acaaagaatt
     8941 tgttgccgat acgtttttcc ccgttattac agctgacaaa tggcaggtcg aaaaagaagc
     9001 ccaacaacag gaagaacacc ctcaagggct taaataccat tttgtcatct ataaaaaaag
     9061 taactcattt taatcaaatt taaccaaaca accatcttgt tttgttgttt tgattattac
     9121 tttgcataaa aaaaatgcca acttttgtgg gatacaacat attttcgaaa gacaaaacaa
     9181 agctgcactt taaaatatgg gacgttgctc aacctaagat atgtatatgc attgtccacg
     9241 gattgggcga acatagcggc aggtacgaag agtgggcaaa tatgtttggc gaaaaaaata
     9301 ttgctgtagc ctcatttgat tttcgcggcc atggattatc tgacggcaaa cgaggccacg
     9361 ctttttcttt tcataaatta tatgatgata tagatgcatt tttaactgaa gtaaaaagaa
     9421 ggtttccgct tgtaccgctt gtactgtacg gacatagcct tggagggagc ctggtactta
     9481 actattacat gtcaggcaaa agctatcaca cctcggccat aattgttacc tctccctggt
     9541 ttaaattagt caacgaaccc tcaaaatttt taatagactt tactatggcg ttaagccgtt
     9601 ttttgccttt tatcactgtt tcaaacagga taaacccctt gttgttatcg cgcgacaggc
     9661 aaatagccga agaatacaca aaagacccct tggtacacaa caaaatatct ttacggctat
     9721 ttcatgaagc ataccggatg ggaataaaag cctcaaaaca agcttataaa ctcaatatcc
     9781 ccaccctgat catgcacgga gaaaacgatg ccataacatc atctgctaca tctgtagagt
     9841 ttgcaagaaa caccggccct tttttatcat tacacgacct ttgcaaaaaa atggtttttc
     9901 aacacatcct ccattggttg caggtaaaca acctttacag ggaagataat agcccaaaaa
     9961 atgttgtcat tcagaactat tattgaaata aacccttcgg acagcaagat atcacacaac
    10021 actccaatgt gcatgatggg ttcgtgcttt gcaaccaaca ttggggaaaa actggaaagc
    10081 ctgaaatttg acatcctgct caaccctttt gggatattgt ttaacccgct ttcgatagcc
    10141 gcctctttaa aaagaactgt cgaaaacaat ccatttacgg aacaagaact tgtttttcag
    10201 cacgaaatgt ggcattcgct tgctcatcac ggaaagtttt cgcacacgga taaaagcaaa
    10261 tgccttgaga atatcaattc ttcgctggca gaagcccacg aaatgatact aaaaagcaag
    10321 ttcctcatca tgacctttgg cacagcctgg atttatttct acaaaaaaac aggcaatgta
    10381 gtagccaatt gtcataagct accttcctca gcgtttgaca ggcgcctggt ttctgtagaa
    10441 gaagtatata caaatgtttt tgactcactt tcgttaataa aaaaaatcaa tcccggcatc
    10501 cagtttatct ttaccgtaag cccggtgagg catctggctg atggggccca tgcaaaccag
    10561 ctaagtaaag ctactttact actggctgtt tctcagttat gcaatacttt tgaagcgcaa
    10621 tattttcctg cttatgaaat tattttggac gaattgcgcg actacaggtt ctttgccgat
    10681 gatatggctc atccatcgcc tttggctgtt gattatgttt ttgagcgttt tgctgaaacc
    10741 tattttaata aagaaacccg gcatttaaac ctcaaaattg atgaaatata taaagccgtc
    10801 aaccataggc cggtaaatga aaatagcata gctcatgaaa aatttaaaga aatcatgcta
    10861 aaaaagattg aagacattga aaaggccaat ccttatcttg atttctcgga agaaaaaaag
    10921 cattttatac taaaccgcta ataaactcaa tgaaataaaa ccgtttagca ggtttaggta
    10981 aaaccaacag attaacaatt tcagattaag agtaaacgaa ttattctgaa gttcgtgttg
    11041 accacattcg ggatcagata aacaagaaaa ataaaaccgc cccaaaatta cttttgagac
    11101 ggtttcaact atccttttat taaaaattat cccaacacta cttcaacatt gtaagtcttc
    11161 ccggcaggct gaagagggat catatttccg cttatttctt ttccatctac agtgagcctc
    11221 tttacacctt tttgtacttt atcagggttg cttactttaa ttacatatat ggccccacgg
    11281 aatttgcgtg ttacagtaaa gccttcccag ctcgaaggga tacaagggtc aacaataagc
    11341 ccgtcataat cagctcttac tccaaggata tattgggtaa tactggtaaa tgtccatgct
    11401 gcacaacctg tcaaccatga gtttttggct tcacccggac gaaaagcgtc tttaccggca
    11461 atcatctggg catatacgta aggctcggtt ttatgcaggt cgctaatatc ttcgaggtat
    11521 gaaggacata ttttcttgta atattcccag gcacgttcgc cattgccgtt taccgtttcg
    11581 ccaatgataa tccaagggtt gttgtggcag aaaataccgg cattttcttt gtatccggca
    11641 ggataacttg atatttcgcc gtattcaata tagtattttg tgaaggcagg attgttgagc
    11701 acaattccat atttgcagtc gaggcgttct ttaaccgcat cgagggcttt ggtgcaaagg
    11761 ccttcttctt ttccaatacc tgccatggta caccaaccat ttgattcgat aaatattttg
    11821 ccttcttcgt tttcgttgct tccaactttt ctgccatagt aatcataagc gcggaggaac
    11881 cactctccgt cccaaccatg ctttttgata gcagcaacca tattgtccac ttctttttgt
    11941 gcacgggctg cttcatcatt cttgccaatt tttttacaaa gctcaacata ttcacggcca
    12001 tatacaacaa acaaaccggc aatcattact gattcagctt ttgaaccttc cgttttgttt
    12061 tctgtagtct ggaatgactc gttagggtcg ttcgagaagc agttgaggtt gaggcaatcg
    12121 ttccagtcgg cacggccaat aagtggcaag ccgtgaggcc cgaggttgtt caccacgtgg
    12181 tcgaaagata cagtaaggtg gtcgaacatg gtgcgggcca cagacatatc gttgtcaaag
    12241 ggtaccattt cgtcgagaat gctgaaatcg ccagattcct tgatatagct gattgttcca
    12301 aggattaacc acattgggtc gtcgttgaag cctccaccga tgtcgttgtt accgcgtttg
    12361 gtaagcggtt ggtactggtg gtagcagcca ccatcaggga attgggtaga agcaatgtcg
    12421 ataatacgtt cgcgggcacg ttcagggatt tggtgcacaa aaccaatcaa atcctggtta
    12481 gagtcacgga agcccattcc acggccaata cccgattcga aatacgaagc agaacgtgag
    12541 aagcagaagg taatcataca ctggtactgg ttccagatgt tgaccatgcg gtcgagcttt
    12601 tcatcgcccg aaacaacatt gtaaacgctc agcaggttgt cccaatactt gcgcaattcg
    12661 gccaaagcag cgtcaacttt ttcaggagta tcaaactttg caatggtttc tttagctttc
    12721 tttttattaa tgatattttt agattcccat ttttcgtcct ctttgttttc aacatacccc
    12781 aacacgaaaa cgaagtcttt gctttcgccc ggcttcagtt ctacttcgag gtaatgagaa
    12841 gctattggag accatccatg agcttcagtg ttgcggggtt tgccttcggc aactacctga
    12901 ggttcgtcaa aaccattgta taagccaaag aacgattcgc ggtcggtatc aaagccctga
    12961 attggggcat taactgaata atatgcgtaa tggtcgcgac gttctttgta ttcagttttg
    13021 tggtaaataa cagagccttc aatttcaact tcaccggtag aaaagttacg ttggaagttt
    13081 tccatatcag cagcagcatt ccacaagcac cattcaacaa acgaaaaaac cttaagtttt
    13141 ttggtctctt tgctgttatt ggtcaaagta agcttttgta cctcagccca ggtttttaaa
    13201 ggaacgaaat ataaaacgtt agctgtaacg ccgtttttca cgcctttgat gttggtgtaa
    13261 ttcatcccgt ggcggcattc gtagttgtcg agctctgttt tgcatggttt ccagcctgga
    13321 gaccatatag tatctccatc tttgatataa aaatatttcc cgccactatc catagggacg
    13381 ttattgtagc gatagcgggt taaacgacgg aacttggcat ctttgtaaaa tgtgtatccg
    13441 ccggcggtat tagaaatgag cgagaaaaag tcttcattgc cgagatagtt tatccacggc
    13501 catggagtgc gcgggttggt aataacatac tcccttgctt tgtcgtcaaa aaaaccgtac
    13561 ttcataaatt gtggtgttta aaaaaatatt gattaattta ttttttgatg tctttaattt
    13621 ttagttgaat agaggtttgc ccccggtatt cgttttcttc aatgcaatag caaatgtcaa
    13681 acgaatagcc tttactgata taagggtaat ggtttgaaag gttgaaacct attgcggggt
    13741 atggcttaaa aggctcatct tcatggataa tattgagctt caggtgttct ttgttgcttc
    13801 caacaatgcg gccggtccca tcgtccacta cattttcggt aaaaaacaca ggtgccatgt
    13861 tgtttggccc aaatggttca aactgtttga tgattcggta tagtttaggc gtaatatctt
    13921 tcaattttag ttccatatcc acttcaatac aagggattag ctgttcgttc atcacgcttt
    13981 ctgcaactac ttcttcaaat tttatgcgaa aagctttgaa gttttctttt ttcatggtca
    14041 aacctgcagc atacatgtgc cctccaaaat tttcgagcaa gtggctacag ccttctattg
    14101 ctttgtacag gtcaaaccct gttacagacc ttgccgaacc tgtaataagc ccatttgact
    14161 ctgtaaggac tatggtaggc ctgtaatatg tttcgattaa cctcgaagct acaatgccaa
    14221 caacgccttt atgccacgat gggttaaaaa gtaccgttgt cttgcggtta tgttgatctt
    14281 tatcggaagc aatcatatcc aatgcctctt tggtaatgtt gctgtctata tttcggcgtg
    14341 tattgttaaa atcgttgatc atatcgccca tatcgagtgc taatgcttcg ttttcggcaa
    14401 taagtagttc tacagacgaa gtccctgttt caattcggcc ggcggcatta atacggggcc
    14461 ctattttaaa aacaatatca tcaatacaaa tttctttatt ttcaataccg gctatttgta
    14521 tgatagcttt caagccacta cgcggggccg agttcagttg ttttagccca aaataagcca
    14581 aaatgcggtt ttcgcctgtg atagggacaa tgtctgaagc aatagatact actaccaaat
    14641 cgagcatctc gtataggggc tcaaaactat agctgttttt catgcaaaat gcctggagaa
    14701 gcttgaaccc aacaccgcag ccagaaagct ctttaaaagg gtaactacag tcaggccttt
    14761 tgggatctaa cactgcataa gcttcaggga ttgcagcccc ggcagtatga tggtcgcaga
    14821 taataaaatc tatacctaaa cttttggcat acgcaatttt ttctacagct ttaatgccac
    14881 agtcaagggc tattaccaga gtaacaccat cagaagcagc caaatcaata cttttaattg
    14941 atattccata tccttcggtg tatctgtcgg gaatgtagta agatagtttt ttgatatact
    15001 tttttaagaa ggtgtacatc atggctaccg aagtagtccc gtccacatcg tagtcgccat
    15061 acaccatcac tttctctcca ttgtcaatgg ctgcttgcaa tcgttcaacc gccttgccca
    15121 tatctttcat cagaaacgga tcgtgcaggt tttcgagctc tggcctgaaa aacatctttg
    15181 cttcctcgaa agtattgata cctcgctgaa ccaaaagatt tgctattgga tattctacgc
    15241 ccaatacttg catcaggtgt tctacttctt cagtcttacc gggtgtatga attatccagt
    15301 ttttctccat ttacaggctt ttcaggtttt atctattttt caaatttgtc ctttttattg
    15361 aaccttcaaa gatatatttt ctgatacaac aattaatgcc atggctttat ttttttattc
    15421 aaaattcaac gcaaattgtt tatcagagta aggattaaca acataaaagc gctttatgtt
    15481 ttttagacgt ttatttattt ttgaggttaa aaatttatgg gtcaataaaa tgcgagaata
    15541 gtagatattt tctactctcc tctatttgaa ttgtctttcc aactgtttta ccaatctttt
    15601 agctatattt gctaacaacc tgatatttca atgaacatta tgatgaaaaa tatactcttt
    15661 gcattttgca ttataattgc tttaattgca acaagttgca aaaaagacaa ggccgattct
    15721 gccactatat ctgagcttga tatatctatc aatcaattta tttgggacat tatgagtact
    15781 aactatttat gggcagacaa tgtgcctgcc ttaaatagca gcaattacag ttccactatt
    15841 gatattgaag aaaaactgct aaaaaaatat agcgaccaca aaaaattttt tgatactttg
    15901 ttgtattaca aaggctctgt tgacaaatac tcgtttatta tagatgacta tgagaagttt
    15961 gaagcatcaa ggcaaggtta tgctaagtca atggggtttt attacaattt atatttattt
    16021 aacaattata aaaatgacga caggcttttt gggttagtaa tgtatgttgt aaaaggaggg
    16081 ccggcagata aggcagggat taaacggggc gatttttttt tcataattga tgatacccag
    16141 ctcaccatca ataattacca aaatcttttg tcaaaagaat cttataaaat cagcttagct
    16201 tattatcatg cttataacaa agaaataacc gttacaggga catcatgtaa ccttacagct
    16261 gaggcattac aagaaaaccc gatttttttg gacactattt attctattaa ttcacgcaaa
    16321 ataggttatc tgatatataa ctattttgat ccaaactttg atagccggct caatgccata
    16381 tttgctaaat ttaaacaaga gaaaatcagc gatttaatcc ttgaccttcg gtataatgga
    16441 ggaggggctt attcttcggc tatcaatctg gccagcatga tttatagtac tgacaatacg
    16501 aaggtttttg ttaagaatga atacaatcaa aaattacagc agaaattgct tgaaaaatat
    16561 ggcgaaggtt tttttaaggc ctgttttaca agtaaaataa taaaaggaga tgcgataact
    16621 gaagaaaaca ttaattcttt aggactttca aaaatatata ttattacaac ccagtcaact
    16681 gcttcaacaa gcgaaatgtt gataaataac cttatgccat acataaaagt atataccttt
    16741 ggtaataaaa cctttggata cggagtgggt ttaatgtatt tcaaagatta cgaccaacta
    16801 aaccgactaa ataccaggca caaatatgca atgcaaattg tagtgctaaa aacgctcaat
    16861 gttaatggga atggatttaa cacagtgggt atagaaccat atattgccgt tgacgaaaat
    16921 tacaccagtc ttttaccttt gggagataga aatgaaacat tgcttaatga agtcatttct
    16981 aatattttct catcaaaatc tgcaaagctt aataatccaa ccatttttga tttttatgcc
    17041 gacaaaaacg accttatccc tcacaaaaaa gagatattta tgaatactat ctctttcaag
    17101 aagtgatata gatgatttat aacgcaaggt tttaaatatt tgtcctgagg atgtagtcca
    17161 agtcaaaaac ctaaactata agatttttct ttcgtaccta cggtactttt attacatcgt
    17221 atttgcattt tactaccata ctattggccc tccgggtctt ttgtgaaaag gacttagcga
    17281 taatcagtac aagttttgaa aattttgtac cataaagctg tagtagtatt gtacgcgtta
    17341 caaacgcgca caaccgagcc ctccgggtct tttgtgatat tatattttgt cacaaaacaa
    17401 taaacccggc caggaatcct gaccgggttt atttttatag taaaaaacta aatcgtgatt
    17461 atagttttac caaacctttt ttggtgccta cccatgcttt cccatctttg tcaaaagcta
    17521 tggcagtaac ataaagatta ggcaagcctg atgtttttgc agtccaagtt tggttctcta
    17581 aaatggcaag gcctttttcg gtacctaccc ataagcggcc gtctttacct tcggcaatag
    17641 catgaatacg ggcacttgga agatcatttt ccgtaaagcc aaaaatgtca gggcttttgg
    17701 tttcatgccc tacaataaac tgtaacccac tattgccacc catccaaata cggttttggc
    17761 tgtcaacaaa cacagcattc attgtatctg ataagcatgg gccgttgtaa ggtgaagata
    17821 actgcgaagc tccggtaaag gcatctaagc tttcgttgta gccataagcc cggtaaatga
    17881 ttttgccgct ggtagcaaaa tatacatctc catcccagct ggcaatggca tttacctgag
    17941 atttagcaaa ttcaaggttt atatattcat ccaaagcatt aaccaacagg ttttcttttt
    18001 tccacttatc gtctttgttc atggtagggc ctgaagctgt gccaaaccaa cggattgact
    18061 ttgcatcaat atggctggca taaactttat tgcttgaaag ctttgccgca tcaatgcctg
    18121 tgcctgacaa tacaccatcg gtaatagtgc atttggtaag gccattagaa gtgccaaccc
    18181 acaacgtgtt agatgctttc tcgtatccaa gagaaagaat ctttgtggca gctactacat
    18241 cctgcaattc gtatccggct tcgacttgac aatacaaacc gctatcagtc ccaacccaaa
    18301 gtttattaaa attgtctaaa acaatagagg ttatttcttt gttgtcgagg tttgacaaca
    18361 ctacttttgt cttttctgga tcatcatcac tacaagcagt aaaaaaaact gcaaaagaaa
    18421 aatataaaag cttttttatc atatcattat tttttagtaa acacaaatgt ataaaataca
    18481 tttattttcg acattatctt acttgaagtt tcaacgattt tatttcgctt ctgccattat
    18541 accgggcttt gagtacacat acataagcac cgcttttgag tttgcttgca tcaaaggtct
    18601 ttgtatatga accggcagct aattgttttt gttcaatata ctcaatcata cggccgtctg
    18661 ttgatgctat aaataacgaa actgaagcac tggcttttaa ctgataagtg ataaaacagc
    18721 tattaaccac aggatttggg tatgcattga gagatgcctc aacttgcgat actttgatgg
    18781 ccgttttatt agataccaat cctgaaggat acgaaactaa ttctatacct ttgccgtctg
    18841 cacttatatt tcttgaatat tgcttattaa tcggattatc ccagtgttca tgaaccccta
    18901 acgatttcat ataagttccg tcgccttcag ggtcatatcc ggcaaatgga tatgatttgc
    18961 catcaatatc tttaaccgta gtaggccagt tagctgggtc tgctgcctgg tgcaggaaat
    19021 catctacagc agggaaaaat gggcggtctt taaatttggt atttttatcg gcctcagccc
    19081 tgagaaaatc taaacaaaca gattccaaag ctacttcgtc ctgagaaaca aagagcgaac
    19141 ttggccagtc gttgttaaac ggggcaatac cccattttac tggcatatca gtagcttcaa
    19201 tgccgctcca taaaccatca acgacaaata gcacagtgtt tcccccaagt ttttcatgtc
    19261 ccataatatc tgtcaatact cggtacatat gataatcacc acgacaatgt gcagctgata
    19321 aatcgtcgtt gccagttttc atatcgccag tagcaataga acctgcatgt aatctttcag
    19381 cacgataact tccaccatga gaactagaac caaaatgatt ttttgcagat aatgatacac
    19441 cactacgagc gtgagctttt aaagcagaaa tattaatcag ataatcggca tcgtacattt
    19501 cctgcatcat atcatcatta attgcagatg gcataacttt gcctttatca gaccaaacaa
    19561 tagcattact tacccatcca gaagaagtag ttcttcctaa tgatgttttg tcatctttat
    19621 caataatgat cactttagga tacattggcc aaacaatatc tgccatactt ttatatacat
    19681 gtgacattgg ctctccaatg atgatgtcgc tttgggcaac gccacagcta tcaatgagct
    19741 gcactaaaaa tgccaaaaca gcctgaggcg aagtgtttga catagcattg ccaacgcctt
    19801 gtcctgaatt acctttttct gacaagtcag agtaaatagc ccaaccagcc tggccgttgt
    19861 tgactttgat aaatattttt tgtccggcaa catagctact ttctgtaccc gttttctttt
    19921 tattaaaact tttgaaaata gcatcccatg aagcttttac tgttgactcg cctccaatca
    19981 acataattgc tttattggca agcatattaa tagtgtcttg gtttgtgttt tttgcctgga
    20041 aataagcatc agactttgta aggtccatta ctccgttcca gcctcctgtt gaaacaatca
    20101 tatttgtaca ttcatccctt gtagaagcag ggttccaggc ccaagcaaca cgaccaggtt
    20161 tgattcccca agctgtaccc attggactgt ttggagcatc aggaaggacg ccacgagtcc
    20221 attgaatacc taaggtattg gcgtaaatct gtttaacatc attgatattg aaaataacca
    20281 caaggacaaa gctaatggca atagccccga tggctgccca atattttgct tttttgaggt
    20341 ttgcaatagc ttttctgaaa aataaaagcg aaccacctaa agaaagcagg taaattacaa
    20401 aacccgacat aagaggtgca gccgtgcgca tacatgggta tgcagcacgt tggggctttg
    20461 gtattacgcg taccaaaaac caaatggtag aag
//
LOCUS       DAOSPF010000061        20076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_2683_length_20076_cov_6.683333, whole genome shotgun sequence.
ACCESSION   DAOSPF010000061 DAOSPF010000000
VERSION     DAOSPF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_20076_cov_6.683333"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(494..1000)
                     /locus_tag="PK252_11295"
     CDS             complement(494..1000)
                     /locus_tag="PK252_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT15334.1"
                     /translation="MTDKVSKINSYLTFKLGDEFFATNVSKVLNILEMTKVTKVPKAP
                     VYMKGVINLRGSVLPLVDTRIKFEMSETVYTPNTCILVLDILLNNESVHVGALVDSVQ
                     EVVEIDDNAIQPAPSIGTRYRTEFIEGVAKINEEFVMILNMDLIFSTEDMSILKESFA
                     ENKEEATA"
     gene            complement(1000..3078)
                     /locus_tag="PK252_11300"
     CDS             complement(1000..3078)
                     /locus_tag="PK252_11300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT15335.1"
                     /translation="MDNFRKKFIEEAYEHINEIEQALLVLERDPENKELIEKVFRAMH
                     SLKGGGAMFGFDRVSEFTHNLENVYDLVRNGQMYVSKELLDVTLQSVDHLKNLLNDDN
                     VEDETVVNQHAALSNQIQKIIDEKVALTQNTEAKKDISAATQAKDVDSQCNTFYIHFL
                     PNESIFDNGTNPLYLIDELHALGECIVLPHFDKIPPYDNYKPTSCYIYWEVYLATKSN
                     INAISDVFIFVEDECKIDIQKLSDTNLLKNKEFVDNIHAQYNKGKEADVLQLHQYSAS
                     AEIQDAKESGTAVKTSEKNVVSAKSNTISSIRVSSEKLDNLMNLVSELVTTQARLSLY
                     ADQDNNPELIAIAENVQKLSRQLRDTAFSIVLIPIENLMTRFQRLVRDLSQDLSKEVQ
                     FIAEGTETELDKTIIENLTDPLLHILRNSLDHGIEPPAIRLKNGKPRQGTILLKAFYS
                     GANVHIQVSDDGAGIDVEKIKQKAISKGIISADANLSNREILDIIFMPGFSTATNITE
                     VSGRGVGMDVVRRKIADIRGEVEIDSKPGQGTVTTIKLPLTLSIIDGLLVKIHDVYYV
                     IPLTSVDKIYAADHSKVYNTFNNLIVLDGEQIPFFYLRNEFELEENTSLGEQIIIVRY
                     EDKRIGLVVDSVVGEYQAVLKPLGKYYKNQEIISGATILGDGTIALVIDTNKAIKFFS
                     QKFAKMEEVK"
     gene            complement(3078..3443)
                     /locus_tag="PK252_11305"
     CDS             complement(3078..3443)
                     /locus_tag="PK252_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15336.1"
                     /translation="MAKTILIVDDSESIREVVSFTLENEGYKVLVGVDGKDALKFLDG
                     SEIDLVITDLHMPVMDGIELIKEIRKMEKYQRIPILFLTTESQAAKKMEAKDAGATGW
                     IIKPFVPAKLIAAINKVIR"
     gene            complement(3470..3775)
                     /locus_tag="PK252_11310"
     CDS             complement(3470..3775)
                     /locus_tag="PK252_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15337.1"
                     /translation="MKAKSSNKETITNDKKHTIVIEKDFSLNTIETIKADIDTILDNN
                     KELHLEFKKIESFDLSSIQFLYALKQKLGNNLSFNLDLKPEIKTIINHNGLDKIINS"
     gene            complement(3795..5597)
                     /locus_tag="PK252_11315"
     CDS             complement(3795..5597)
                     /locus_tag="PK252_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15338.1"
                     /translation="MKELEDKNYIETLLNAITIEEAIASFSLIDDKIIDLLQCSSDDF
                     LTLNDHFKVYHKESKNLSGNANEIIEALTDRNLICSFSELKKIADNFNQISNTFASNI
                     ENFDIELKKIKNKVESIVIIQQSYKQNILSVNVLLANVDKNESLNIGAFDKKIVSFSN
                     LIKEDFLQTEKLISEFALLASEGNNFLDKVKKETYSTLLILNDQIDSSLKLFNKKNQE
                     ASQLIDFFKGLNEKNSGKIANIITNLQYHDIIRQKIEHIQNTHKDIIKELKSIQNNES
                     EVIQLHNKVKIYIKIRDVAGLQAAQLIHANNQYQAAINQISENLETLGNEMITISSTC
                     NNFVSRSEHAKDVYLNTIVELLNNALKYIRQMNQQLDAIKDKIATLKQRKGEIDAVMQ
                     NIHQQKVNIEVELKNININNKYLSQASEVLKETDDLFKKYSTLSKELNQKLGLSENIS
                     ILGQDKLVEAFGQMNNSLPLMIDLLKKSAQTVEEYLSVNTNISLNVSESIKKSLHNIK
                     YYELFEKSSEVIIQELNTLNARLNSGSGELNKISKEENLKLLKSRYTMASEHYIHDQI
                     SNLENGKLTEGDMEEIVKQANEKNEEDDDNLELF"
     gene            complement(5645..6022)
                     /locus_tag="PK252_11320"
     CDS             complement(5645..6022)
                     /locus_tag="PK252_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15339.1"
                     /translation="MKQLDTILVIDDSSTNVVLIEAVLNNKGYQIATALSVKEAYALM
                     DKHKPKLILLDLLMPRINGFDFLKEIKENKNFQDIPVIVVSALTDIETINKTKELGAV
                     GFIKKPIDIHQLLELVENIFVKK"
     gene            6245..6913
                     /locus_tag="PK252_11325"
     CDS             6245..6913
                     /locus_tag="PK252_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT15340.1"
                     /translation="MAINSTTKDIFDLLSEAENKKLQLCSTTVHYRKGDIIFMQKTPA
                     SHVMFIKSGLVKIYREGRMNKSVILKLAMPGSFIGLLSVFGSDVHQYSASTIEPCDIQ
                     IIEAGCFSEIIASNPNVARKIIELISIKGLDLFDRLMSQTHKQLPGRIADVLLYFSDK
                     IYKSHRFSFPLTRRELAELAGTTKESFIRTLTEFKNDKIIELEGSEVEIKSMNLVRTL
                     SELG"
     gene            6927..7295
                     /locus_tag="PK252_11330"
     CDS             6927..7295
                     /locus_tag="PK252_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15341.1"
                     /translation="MKILIVDDIVVNRYIIKELLKKAGYAVVEANNGKEAIDLILKDE
                     IRLIFMDIEMPVMNGIETVHYIRTTMNIPKNKIKIFALSAHNKALIHEQFDMSEFNGV
                     LTKPLNKDKILEIANNAFDF"
     gene            complement(7578..8351)
                     /locus_tag="PK252_11335"
     CDS             complement(7578..8351)
                     /locus_tag="PK252_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOT15342.1"
                     /translation="MKNIALSLIVFIMLFGFMSCSDNGARIPMGGIEVYFSDAPANYD
                     AFNIDIQLIRIKYDSLETIECKPYRQGVYNVLNFSNSNDTLLCNTYIPAGKIKSIEIV
                     LGTNNSIKYKNNTYILTPKIVAGTKHIEIKENSSTRIHLDFDAASSAKIINDTGVDFT
                     PLMYVHIDNTGGSIQGYILPKGYRPFIYAISNKDTFCTYSNQNGKFLFKRLPPNSYQL
                     YYQFVQENGLTLFIEKIEVKPNSLTDAGNVFCTKPGKTR"
     gene            complement(8477..9550)
                     /locus_tag="PK252_11340"
     CDS             complement(8477..9550)
                     /locus_tag="PK252_11340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT15343.1"
                     /translation="MKRLFSLLTTICTPFVILSGTNTNKGHWFEADNSMIKYVGRIDF
                     TDAKAPKFSAAGTYIEVKFKGTTVDIELVDEHKYNNNQFFQVSIDENAPVRFQTTAGQ
                     SIYRIASGLTADTHIVRITKDSEASIGWMQFKKIRVDELLDHTLPSRKIECFGNSITC
                     GAGIYNDLCNISVDSKWYYCTSATKSYGALVAKKLNAQYHLTSWSGIGLTKSCCGMTV
                     TMPTVFDLTFIEGSSPQKWDFSRYIPDVVTICLGQNDGVIDSATFVNAYINFIDKLKI
                     YYPNTQFLCITSPMSDDALYNYHVKCLTAIANHYCNKGDNKVHKVLLSHSMNNGCTTH
                     PDGAQHEIIATELEAAIKNITGW"
     gene            9739..13419
                     /gene="metH"
                     /locus_tag="PK252_11345"
     CDS             9739..13419
                     /gene="metH"
                     /locus_tag="PK252_11345"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT15344.1"
                     /translation="MQESKKLYAELERRILILDGAMGTMIQRFKLGEEQYRGERFKDF
                     QYPLKGNNDILSLTQPDIIRQIHEQYLEAGADIIETNTFNANRISQADYHLEDLTYEI
                     NFESAKIACQAAEKYSKLTPDKLRFVAGSIGPTNKTASMSPDVNDPGYRAVSFDDLVV
                     VYTEQVKGLLDGGVDILLVETIFDTLNAKAALFAIDELLKQRNMRFPIMVSGTITDAS
                     GRMLAGQTVEAFLNSVSHIDLLSIGLNCALGAAEMRPYIEELSQKSNFYISAHPNAGL
                     PNQFGLYDETPEQMAHHIHDFADHHFVNIVGGCCGTTPDHIRQFAKSVEGAKPRLKPA
                     LEPVTKLSGLEALSITKEANFINIGERTNVAGSKKFARLIKEEKFDEALAIARQQVEN
                     GAQVIDVNMDDAMIDAEKAMVRFLNLLMAEPDIAKVPVMVDSSKWEVIEAGLKCLQGK
                     CIVNSISLKEGEEKFLYQASRIKQYGAAVVVMAFDEKGQADSFARRTEICGRAYKLLT
                     EKIHFPAQDIIFDPNVLAIATGIEEHNNYAVDFIETVKWIKANLPNAKISGGISNLSF
                     SFRGNDVVREAIHSVFLYYAIKEGLDMGIVNAGNMKVYDDIPADLLERVEDVVLNKRP
                     DATERLIEFAEKIKSTGDKTETIEEWRKYSLEERLKHALVKGIDNYLETDISEAIEKY
                     PKALEIIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKKAVAILMPVIEAQKGNNG
                     NISSAGKILLATVKGDVHDIGKNIVGVVLACNNYEVIDLGVMVPAEKILEVAKKEKVD
                     IIGLSGLITPSLEEMVHVASEMQRQGFDIPLLIGGATTSKIHTAVKLEPVYEHPIIHV
                     KDASKSVPVVSNLLSKSLHKNFIEEVRREYDNLRTSYNITSSAGNYVSLEQARANKVK
                     IDWNIEKPFKPMFVGTKVWNNYPIEDIRKYINWMFFFIVWQLKGKYPEILQDPEKGAE
                     ARKLFNDANKLLDELQSKRLIKANAVFGIYPANAVGDDIEVYTDESRTEVFKVFRNLR
                     NQAKQNVNEPNACLSDFIAPKESGIKDYIGAFAVTAGIGVDKLIAEAKENHDDYKVIM
                     LEALADRLAEAFTELLHEKVRKEIWGYASSEALSVDDMLLEKFKGIRPAHGYPACPDH
                     SEKQTLFELLQASKNSGISLTESNMMLPAASVSGLIFAHPRSQYFFVDKIGKDQAEDY
                     AKRKGVPVGQVEKWLNANLNYK"
     gene            complement(13454..14254)
                     /gene="pgeF"
                     /locus_tag="PK252_11350"
     CDS             complement(13454..14254)
                     /gene="pgeF"
                     /locus_tag="PK252_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOT15345.1"
                     /translation="MRAVTSKHTQLYQFEIFDYFHEVKHFVSTRIGGYSQPPFHGFNL
                     GFGVEDDTQVVLKNRMALAESVGIPFDWFVFPRQTHSVNVAKVDTSHCGLGTKDRESA
                     IADTDALTTNEKNVLLIVQVADCVPILLLDSKNAAIAAIHAGWKGTVGEIVVNTINKM
                     NQQYGTQAEDIIAAIGPSAGPCCYEVGDEVLDKVNKNKFDKVVKKHNESKILNLWEYN
                     RILLIESGVKPENIEVSNLCTICNDDKFFSSRKGKGVTGRFVAGIMLQ"
     gene            14346..14876
                     /gene="cysC"
                     /locus_tag="PK252_11355"
     CDS             14346..14876
                     /gene="cysC"
                     /locus_tag="PK252_11355"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOT15346.1"
                     /translation="MVIHKPLSIWFTGLPGSGKSTLADALTLVLKGYNYPTILLDADI
                     VRKGFNSDLGYSLDDRKENIRRVAELSKILIEQEIFGIQAFICPTNELRNMAREIVGF
                     NNFFLIYLSTPLEICEQRDPKGMYAKARAGYMSQFTGVSQEFEVPDTAHITLDTSILT
                     IEECIQSIMERIGRFN"
     gene            complement(14950..17316)
                     /locus_tag="PK252_11360"
     CDS             complement(14950..17316)
                     /locus_tag="PK252_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15347.1"
                     /translation="MKRVFTFLLLPLLVVSALEAQFSFTGTIKNEHNEPLAGATVVVD
                     NKGFVADENGAFKIDNIEKGQKEVKVVYLGYNTYFENIDFTSDQTRNIILKENVTNFE
                     EVVVSAIRAQNFTPTTFTTIKKQDIKQLALGQDAPMILSSQTSVVSTSDAGNGIGYTA
                     MRIRGIEGRKINVTINGVPLNDPESQGVYWVDVPDIATSSQSIQIQRGVGTSTNGSGA
                     FGASINLQTDYISQTPFVEYSGAYGSFNTRKNSLHVGTGLYKHHFFFEGKVTGIKSDG
                     YIDRARSDLKSYFAQAGYYSDKTIVKLVGFGGQEETYQAWYGIDSVSIKSKGRTYNPV
                     GEYFLPNDTVPHYYDDMVDHYSQDHIQLHVMHSFSSNLTFNAALHYTYGRGYYQDLSP
                     GWYEQSYFKLPNGINGNDTISYSDVIQRLWLDNHFYGGIWGLTYTANKYTLIWGGAYN
                     RYDPAKHYGQVMWARYFLDGMQGMHFYDNQGRKTDFNTFLKASYSITNEISAFADIQY
                     RNIYYKAWGTNREYANDTISIKKQYNFINPKVGLLYQPTKSWVVYTSFAVANREPSRS
                     DFVDAEVGEEPKPEKLYDMELGTRYSDDQFNFELNFYNMQYRDQLVLTGELNNVGTPI
                     RKNVGKSYRMGVEMTGGIKLLDVIKLEGNVTLSKNRTGYNESVDDSVIYYKDKIISFS
                     PSVIAAGKITYLTPVKGLELFINTKHVGKQYLDNTENKIRSLNAYTVTDGGINYSTII
                     MGTDWQFNFRVNNIFDEMYNSNGSVGSYSVNYYPQAGTNFMAGLTVKF"
     regulatory      complement(17381..17477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17570..18469
                     /locus_tag="PK252_11365"
     CDS             17570..18469
                     /locus_tag="PK252_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOT15348.1"
                     /translation="MEQKRKIAFIINPVSGTTSKAEMPSMIESIIDKSKYDVCIKFTT
                     CAGNGHELAKGFVAEGYDIVVSVGGDGTMNEIASALTETNTALGLIPCGSGNGLARHL
                     GIPLDHKQAIALFNDCKIIKMDYGKANDVKFFCTCGAGFDAHIGHTFASAGKRGFLTY
                     LRSIFTEFRKYKPKKYKFKTEEGKFKKKAFLVTFANASQYGNNAYIAPNADIQDGLID
                     VCILEPFPKFRALGIGFKLMSKKIDKCRYVQIMQTDKVRIKTKKNVVFHYDGEPCIMD
                     RKVKISAVQKGLNIAVLSNSPLN"
     gene            18476..18976
                     /gene="folK"
                     /locus_tag="PK252_11370"
     CDS             18476..18976
                     /gene="folK"
                     /locus_tag="PK252_11370"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT15349.1"
                     /translation="MAEVYLSLGSNIGDRLENINLAIDYINENIGEVICCSSVYETEA
                     WGINSSGMYLNQVILIKSNIKPISILEKIWDIESKLGRVRVVDNKYADRTIDIDILFY
                     DNHVIHGDYVTIPHPMLQHRRFVLVPLCDIAADFVHPELNKKVADLLQVCKDDLKVQK
                     FDPGFN"
     gene            19120..>20076
                     /locus_tag="PK252_11375"
     CDS             19120..>20076
                     /locus_tag="PK252_11375"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT15350.1"
                     /translation="MGKLQTEIKYITGIGPKRAEILNKDIEVFTIKDLLYYFPYKYID
                     RTKFYSIAEVNADLPYIQIKGKITKFNTIGEGAKKRLTALFTDGTGFIELVWFKGIKW
                     VMLQYKPDIEYIVYGKPTQFGHILNIAHPEIDEVSKAEQVTATALQPQYNTSERMKSN
                     FLTSRTIHKLIASCLKFTINEIEETFPEYLMKTCKLIPLKEALTQIHFPTSTELLTKA
                     EFRLKFEELFTLQLSLLRQKKYRQQVHQGYIFSNVGHYVNTFYKHCLPFELTNAQKRV
                     IKEIRQDLGSGKQMNRLLQGDVGSGKTLVALMISLIALDNNLQ"
BASE COUNT         6042 a         3589 c         3864 g         6581 t
ORIGIN      
        1 aaaaagtaat tatccaaatt atactaattg ccaatcgaga attttttttt atagacatat
       61 tattattttt ttagtattat ttctcaaatc gattagctat tattgtacct aataattcgc
      121 tgacaaacaa acagaaaaca atctttacag atagcaaatt tttatactac tatctttagt
      181 taatttttct gataaaaaac ctaacgtaat ggttaataag tatatagctt aatttttcat
      241 caaaaaatgc acctttaatc aaagcttttc ggctttgttc tttatttttc ctgatattta
      301 aggtctctct tttttgtggc agcagactat tagatttatt aaatccctgt aactttcaaa
      361 gataaaagtt ttataatgac attactgtta tttatgtcaa aaaaatacct gtgaaatgaa
      421 tttgattaca ttaatacaaa aggttaatca aattcctcaa acttaaatat tagcaacgac
      481 tctaaaagta tttttatgcc gtagcttctt ctttattttc agcaaatgat tctttcaaaa
      541 tactcatatc ttcggtcgaa aaaatcaagt ccatattaag gatcatcaca aactcttcgt
      601 ttattttggc taccccttca ataaactcag tacggtaacg ggttccaata cttggggcag
      661 gctgaatggc attatcatca atttctacca cttcttgtac ggaatctact aaagcaccca
      721 cgtggacaga ttcattattg agaagtatat caagtaccaa aatacaggta ttgggtgtat
      781 ataccgtttc ggacatttca aatttgatgc gggtatccac aagtggcaaa accgaacctc
      841 tgaggttaat gacccctttc atgtaaaccg gggctttggg cactttggtt actttggtca
      901 tttctaatat gttcaacact ttgcttacat tagtggcaaa aaactcgtcc ccaagtttaa
      961 aagtcaaata cgaattgatt tttgatactt tgtcagtcat tattttactt cctccatttt
     1021 tgcaaatttc tgtgagaaaa atttgattgc tttatttgta tctatcacaa gtgcaatagt
     1081 tccatcccct aaaattgtag cgccagaaat tatttcctga tttttataat atttacctaa
     1141 aggtttcaat actgcctgat attcgcccac aacgctatca acaacaagcc cgatacgttt
     1201 gtcttcatat cttacaatga tgatttgttc gcccagggaa gtgttttctt caagttcaaa
     1261 ttcattacgt aagtaaaaga atggaatttg ctcaccatca aggactatca ggttattaaa
     1321 tgtattatac accttactat ggtcagcagc atagattttg tccaccgagg taagcgggat
     1381 aacatagtaa acgtcatgta tcttgaccaa aagaccatca atgatagaca aagttaaagg
     1441 taacttgata gtggtaacag ttccttgtcc gggtttagaa tcaatttcga cttcacccct
     1501 gatatcggca attttgcgtc tgactacatc catacctacc ccgcggccag aaacttcagt
     1561 aatgttagta gctgttgaaa agccaggcat aaagataatg tccagaattt cgcggttaga
     1621 aagattagca tcagcactta ttataccctt agaaattgct ttttgtttga ttttttcaac
     1681 atcaataccc gccccgtcat ctgatacctg aatatggacg tttgcccccg aataaaaagc
     1741 ttttaataat attgtccctt gccggggttt accatttttt aaacgtatgg cagggggttc
     1801 aataccatga tctaaactat ttcttaaaat atggagaaga ggatcggtaa ggttttctat
     1861 aatggtttta tcaagctctg tttctgtccc ttcggcaata aattgtactt ctttgctcaa
     1921 atcctgggac aaatcacgta ccaaccgctg aaagcgggtc atcaggtttt caatagggat
     1981 aagcacaata ctaaacgctg tatcccgcaa ttgacgtgaa agtttttgga cattctcagc
     2041 aatggcgata agttcgggat tattatcctg atcggcataa aggctaagcc gggcttgagt
     2101 ggtaaccagt tcgctcacca ggttcatcag gttgtcgagt ttttcggatg aaacacggat
     2161 actcgaaatg gtattacttt ttgcgcttac aacgtttttc tcagaagttt taactgcagt
     2221 tccggactct ttggcatctt gaatctcagc agaagccgaa tattgatgaa gctgcaatac
     2281 atctgcttct tttcctttgt tgtattgagc atgtatattg tcaacaaatt ctttattctt
     2341 aagcagattg gtgtcactca gtttctgaat atcaatcttg cattcatctt caacaaatat
     2401 aaaaacatca gaaatggcgt ttatgttgct tttggtagcc agataaactt cccaatatat
     2461 ataacagctt gtaggtttgt aattatcgta aggggggatt ttgtcaaaat ggggcaaaac
     2521 aatgcattct cccaaggcgt gcaattcatc tatgaggtat agcgggtttg tgccattgtc
     2581 aaaaatgctt tcgttgggca aaaagtggat gtaaaaagta ttgcactgag aatccacatc
     2641 ttttgcctga gttgcggcag aaatatcttt ttttgcttca gtattttgag ttaaagcaac
     2701 tttttcgtca ataatttttt gaatctggtt tgataatgct gcatgttggt ttacaacagt
     2761 ttcatcctca acattatcat cattgagcag gttttttaaa tggtcaacag actgaagggt
     2821 aacatccaaa agttcttttg aaacgtacat ctggccattt ctgaccaagt cgtatacatt
     2881 ttctaagtta tgggtaaatt cactgaccct gtcaaagcca aacatagccc ctccgccttt
     2941 gagtgagtgc atggccctga acaccttctc tatcagttct ttattttcgg ggtcgcgctc
     3001 gagcacaagc aacgcctgct ctatttcgtt gatatgctca taagcttctt caataaattt
     3061 ttttcggaaa ttatccatta gcgtattact ttatttattg cagctataag ttttgccgga
     3121 acaaaaggtt tgattatcca accggtagcg ccggcatctt ttgcttccat ttttttggca
     3181 gcttgagact cggtagtaag aaaaaggatt gggatacgtt gatatttttc catctttcgg
     3241 atttctttta tcaattctat accatccatt acaggcatgt gcaagtctgt aattacaagg
     3301 tcgatttcag agccatctaa aaatttaagg gcatcttttc catcgacacc aactaatacc
     3361 ttatatcctt cattttccaa ggtaaagctt actacttctc taatgctttc tgaatcgtca
     3421 actattaaaa ttgtttttgc catggtaatg gtatttgctg ttaaaaatat taagaattta
     3481 ttattttatc caaaccgtta tggttaatta ttgtttttat ttcgggtttt aaatctaagt
     3541 taaaactaag attgttgcct aatttttgct ttagtgcata taaaaattga atagaactca
     3601 aatcaaaact ttcaattttt ttgaattcta aatgtagctc tttgttgttg tccaaaatgg
     3661 tatcaatgtc tgcttttatt gtttcgatgg tgtttaatga aaaatctttc tcgataacaa
     3721 tagtatgttt tttgtcgttt gtaatagttt ctttattaga acttttggct ttcatttttt
     3781 tattttaaag ttaatcaaaa taattccaaa ttatcatcgt cttcttcatt tttttcattg
     3841 gcctgcttta caatctcttc catgtctcct tctgtaagct ttccattttc aaggtttgag
     3901 atttgatcgt ggatataatg ctcactggcc attgtatatc tggattttaa aagtttcaaa
     3961 ttttcttctt tgctgatttt attaagctct cctgagccac tattgagacg ggcattgagc
     4021 gtattgagct cttggattat tacttcgctg gacttctcga aaagctcata atacttgata
     4081 ttgtgcagtg atttttttat gctctcggaa acattaaggc taatgtttgt atttaccgac
     4141 aagtattctt caactgtttg ggcgcttttt ttgagcaaat caatcattag aggcaaactg
     4201 ttgttcatct ggccaaaggc ttcaacaagt ttgtcttgtc caaggatact tatattttct
     4261 gaaaggccaa gtttttgatt taattctttt gaaagcgttg aatatttctt gaataaatca
     4321 tcggtttctt ttagaacttc tgatgcctgg cttaaatatt tattgttaat atttatattt
     4381 tttaattcaa cttcaatatt gaccttttgc tgatgaatgt tctgcataac ggcatctatc
     4441 tcacctttgc gttgcttcag tgtagctatc ttatctttta tagcatcaag ctgttggttc
     4501 atctgcctga tatatttcag ggcattgtta agcaattcta ctattgtatt taggtaaaca
     4561 tctttggcgt gttcagatct gcttacaaag ttattgcagg tgctgcttat agtaatcatc
     4621 tcatttccaa gagtttcgag attttccgaa atctggttga ttgctgcctg atattggtta
     4681 ttggcatgta tgagctgagc tgcctgtaaa cctgctacat ccctgatttt tatgtatatt
     4741 tttaccttat tatgaagctg tatgacttcg ctttcattgt tttgaatgga ctttagctct
     4801 tttataatat ctttatgagt attctgaatg tgttctattt tttgccttat aatatcatga
     4861 tattgcagat tggtaatgat attggctatt ttaccggagt ttttttcatt caggccttta
     4921 aagaaatcga taagctgtga tgcttcctgg ttttttttat taaaaagctt taagcttgaa
     4981 tcaatctgat cattgagaat taacagggta ctgtatgttt ctttttttac cttatcaaga
     5041 aaattattgc cttcagatgc caataaagca aattcgctaa ttagtttttc tgtttgtaaa
     5101 aaatcttctt tgataagatt tgaaaagcta acaatttttt tatcaaaagc ccctatattt
     5161 aggctttcat ttttatcaac attggcaagc aatacattta cagaaaggat attttgcttg
     5221 taactttgct gaatgataac gattgactct actttgtttt ttattttctt aagctcaata
     5281 tcaaaattct cgatatttga agcaaaagta tttgatattt ggttgaaatt gtctgcaatt
     5341 ttctttagtt ctgaaaaaga acagattaaa tttctgtctg ttaatgcttc aataatttca
     5401 tttgcgttgc ccgaaagatt ttttgactct ttatggtaaa ctttgaaatg gtcgtttagt
     5461 gtaagaaagt cgtcagaaga acattgtaaa aggtcaatga ttttatcatc tatcaatgaa
     5521 aatgaagcta ttgcctcttc gatagtaata gcatttaaga gggtttctat gtaattttta
     5581 tcttctaact ccttcattat gcaaataaaa caaaagatta tattaaagtg tgaatgtaca
     5641 aaatttattt tttaacaaaa atattttcaa ccaattcaag tagttggtga atatctatag
     5701 gctttttaat aaacccaaca gctccaagtt ctttggtttt attaatagtt tcaatatctg
     5761 ttaaagcaga aactacaata acagggatat cctgaaaatt tttattttct tttatttctt
     5821 tcaaaaagtc aaagccattg atacggggca tgagcaaatc taacaatatg agcttgggtt
     5881 tgtgtttgtc catcaaagcg taagcttctt ttacacttag tgctgtagct atctgataac
     5941 ctttgttatt caaaactgcc tcgatgagta caacgtttgt gctagagtcg tcgataacaa
     6001 gtatagtatc taattgtttc attttaaaca gatttgggtc attcattaaa aaagtcagac
     6061 tattataaaa aaacgttata gtaaatttaa tttttttgcc atccgaaacc gaatgtcaaa
     6121 tataattata aaaatgttta gctatcaaaa ttagtataag cttcattttt ataatataaa
     6181 taatgattaa tatcatcaaa aaagatattt ttatttatct tagtaataaa tatcaaactt
     6241 aaaaatggcc atcaatagta caacaaagga tatttttgat ttgctttctg aggctgagaa
     6301 taaaaagctg cagttgtgca gtactacagt tcattacaga aaaggagata ttatttttat
     6361 gcaaaaaacg ccggcatcgc atgttatgtt cataaaatcg gggcttgtaa aaatatatcg
     6421 tgaagggcga atgaataaaa gcgtaattct caaacttgcc atgccaggtt cttttattgg
     6481 attgctttcg gtatttggca gtgatgtcca ccaatactca gcctcgacaa tagaaccctg
     6541 cgatattcaa attattgaag caggctgttt ttcagagatc atagcatcta atcccaatgt
     6601 tgcaagaaaa atcattgagt taatcagtat aaaagggctc gatttgtttg acaggctgat
     6661 gagccaaacg cacaagcagc ttccggggcg aattgccgat gtattgctct atttttcgga
     6721 taaaatatac aaaagccatc gattttcgtt cccattgacc cggcgcgaac tggcagagct
     6781 tgccgggaca actaaggaaa gttttattcg tacactaaca gagtttaaaa atgataaaat
     6841 tattgaactt gaaggctctg aggtagaaat aaaaagtatg aaccttgtcc ggacgctgag
     6901 tgagcttggt taaaaaagaa aacattgtga aaatattaat tgttgatgac atcgttgtta
     6961 accgctatat cataaaagag ctgcttaaaa aagccggata tgctgtagtt gaagcaaata
     7021 atggcaaaga agccatagac cttatattga aagatgaaat ccgactgatt tttatggata
     7081 ttgaaatgcc tgtaatgaat ggcattgaaa ctgtacatta tattcgtaca accatgaaca
     7141 taccgaagaa taagattaaa atatttgccc tttcagccca caacaaggca cttattcatg
     7201 aacagtttga catgagcgaa tttaatggag tactgacgaa gccattgaac aaagataaaa
     7261 ttcttgaaat agcaaataat gcttttgatt tttaattgtt aattgttaat ttttgtctgg
     7321 taaagtataa tagatgctta ataacactgc gtaatatcgt taatttgaaa ctgttcgccc
     7381 cgctggggct taattttaac gttgtttaat tttactacaa acaggatgtc gctatgcgac
     7441 taaataatag cagcgtagct gcgaactgtt tgtaccagac aacaatgttt ttttaacagg
     7501 gtcataagct ttttatgctt atggactgag gaagataagg ccaataaaaa aaacctgcca
     7561 gagatacttt ttttgaatca tcttgttttg ccgggtttag tacaaaaaac attgccggca
     7621 tcagttaaac tgttgggttt gacctctatt ttttcgataa aaagcgtaag cccattttcc
     7681 tgaacaaact ggtaatatag ttggtagctg tttgggggaa gtctcttaaa cagaaatttt
     7741 ccattttgat tgctataagt gcaaaaagtg tctttgttgg agatggcata aatgaatggc
     7801 ctgtagcctt ttggaagaat gtaaccctga atgctgcccc ctgtgttgtc aatatggaca
     7861 tacattagcg gagtaaaatc tacaccagta tcatttatta tttttgcaga agaggctgcg
     7921 tcaaaatcaa ggtgtattcg agtacttgaa ttttccttaa tctctatatg ctttgtccct
     7981 gctactattt tgggagtcaa tatatatgtg ttgttcttat atttgatgga attattagta
     8041 ccaagaacaa tttctattga ttttatctta cccgcaggga tatatgtgtt gcaaagcaac
     8101 gtatcattac tgttagaaaa gttcaatacg ttgtaaacgc cctgccggta gggtttgcat
     8161 tcaatagttt caaggctgtc gtattttatc ctgattagtt gtatatcaat attgaaagca
     8221 tcataatttg ccggggcatc cgaaaaataa acctcaatgc ctcccatcgg gatgcgggcc
     8281 ccattgtcac tacacgacat aaaaccaaac agcataataa agacgatgag gcttagggct
     8341 atatttttca tttttttgtt tttgcttgtt agcaaagtaa taaaactttt gggagttttt
     8401 tgttttgctg acaactgaaa ataaaaatcc gtttgaagat tatttttcaa gcggctttca
     8461 taaattttat aaatagctac cacccggtga tattcttaat agcagcttcc agctctgttg
     8521 ctattatctc gtgttgggcg ccatcagggt gtgtagtgca tccattgttc atgctgtgcg
     8581 ataacaatac tttatgtact ttgttatcgc ctttattaca gtaatggttc gcaatggcag
     8641 taaggcattt tacatgataa ttgtataatg catcgtcact catagggctg gtgatgcata
     8701 aaaactgggt gttggggtag taaattttca atttgtcaat aaaattgatg tatgcgttga
     8761 caaatgtggc agaatcaatc acaccatcgt tttgaccaag acaaatggtt actacgtccg
     8821 ggatatacct ggagaagtcc catttttgtg gagaactacc ttcgataaag gttaaatcaa
     8881 atactgttgg catagttacc gtcatgccac agcaactttt ggtaagccca attccagacc
     8941 atgatgtaag atggtattga gcgttgagct tttttgctac aagggctccg tatgatttgg
     9001 ttgctgaagt gcagtaatac catttgctat caacagatat attgcataaa tcattataaa
     9061 taccagctcc acaagtgatg gaattgccaa agcattctat ttttcgtgaa ggcaaagtat
     9121 ggtcgagcaa ttcgtcaaca cggattttct taaactgcat ccacccgatg cttgcctcag
     9181 agtctttagt aatgcggaca atgtgtgtat cagctgttaa acctgaagct atgcgataaa
     9241 tagattgtcc ggcagttgtt tgaaacctaa caggagcatt ctcatcaatt gaaacctgaa
     9301 aaaattggtt gttattatat ttatgctcgt caacaagttc aatatctact gttgttcctt
     9361 taaacttaac ttctatatat gttcctgctg cagaaaattt tggagctttt gcatctgtaa
     9421 agtcaattct gcctacatat tttatcatgc tgttgtcagc ttcaaaccaa tgacctttgt
     9481 ttgtatttgt gcctgaaagg ataacgaaag gagtgcaaat agttgtaagt aaagaaaata
     9541 accttttcat gttttttagt ttgttttttt caaaagtaaa gtttaaaagc tgccaatgtt
     9601 aattttgaga tagtattatt ttgattgaat gttcaaagtt ttggtacaaa tgttgaagtt
     9661 ggtacaaatt ctaatgaaag tttgttgatg taataaagac aaacgaattc attatttttg
     9721 caacttaatg taaataaaat gcaggaaagt aaaaaattat acgctgagct cgaacgccgt
     9781 attcttattt tagatggtgc tatgggtacc atgattcaac gttttaagct tggcgaggaa
     9841 caatacagag gggaaaggtt taaagatttt cagtatccgc ttaagggcaa caacgatatt
     9901 ctttcactca cccagccaga tataatccgt caaattcatg agcaatactt agaagcagga
     9961 gctgatatta ttgaaaccaa cacattcaac gccaaccgta tttcgcaggc cgattatcat
    10021 ctggaagacc ttacttatga gataaacttt gaatcagcta agattgcatg tcaggctgca
    10081 gaaaagtatt caaagcttac gcctgataaa ttgaggtttg tagctggttc aattggccct
    10141 accaataaga cggcttccat gtctccagat gtaaatgatc cgggctaccg cgctgtgtca
    10201 tttgacgatt tggttgttgt atataccgag caggtaaaag gcttgcttga tggcggggtt
    10261 gacatcctgc ttgtcgaaac tatttttgat acgctaaatg ctaaagcagc cttatttgct
    10321 attgatgaac tgttgaaaca acgcaatatg cgttttccaa taatggtttc aggtactatt
    10381 accgatgcaa gtggccgtat gttggcaggt caaacagtcg aagctttctt aaactctgta
    10441 tcgcatattg acttgctgag tattgggctc aactgtgctt taggggcagc tgaaatgagg
    10501 ccctacattg aagaattgtc acaaaagtcg aatttttata ttagtgcaca tcctaatgcc
    10561 ggattaccaa accagtttgg gctttacgat gaaacaccag aacaaatggc acatcatatc
    10621 cacgattttg ccgaccacca ttttgtaaac attgttgggg gctgttgtgg tacaaccccg
    10681 gatcatatcc gccagtttgc taaaagtgtt gaaggggcaa agccaaggtt aaaaccagca
    10741 ttggagcctg taactaagct tagtggcctt gaagcgctaa gtattactaa ggaagctaat
    10801 tttatcaata ttggagaacg taccaatgta gccggttcga agaagtttgc acgtcttatt
    10861 aaagaagaaa aatttgacga agctcttgct attgcccgcc aacaggtaga aaacggtgca
    10921 caggttatag atgtcaacat ggacgatgcc atgattgacg cagagaaagc aatggtgcgt
    10981 tttttaaacc ttcttatggc tgaacctgat attgccaaag tacctgtcat ggttgactca
    11041 tcaaaatggg aagtaataga agctggtttg aaatgcctgc aaggtaaatg tattgtcaat
    11101 tcaataagtt taaaagaagg cgaagaaaaa tttctttatc aggcctcacg tataaaacaa
    11161 tatggtgcag ctgttgttgt aatggctttt gatgaaaagg gccaagccga ttcttttgcc
    11221 cgcaggacag agatttgcgg tcgtgcctat aagttgttga ctgaaaagat ccatttcccg
    11281 gcccaggata taatttttga ccccaatgtg cttgctattg ctactggtat tgaagaacat
    11341 aacaactatg cggttgattt tattgaaacc gtgaagtgga taaaggcaaa ccttccaaat
    11401 gccaaaatta gcggcggaat tagcaactta tcattctcat tcaggggcaa tgatgttgtt
    11461 cgggaagcta ttcattcggt attcctttat tatgcaataa aggaaggctt agatatgggt
    11521 attgtcaatg ctggcaatat gaaggtgtat gatgatattc ctgctgacct tttggagagg
    11581 gttgaagatg tagtacttaa taaacgcccc gatgcaactg aacgtttgat tgagtttgct
    11641 gaaaaaataa agagtacagg agataaaact gagacaatag aagaatggcg aaaatattcg
    11701 cttgaagaac gtctgaaaca tgctttggtc aaaggtattg acaattacct tgagaccgat
    11761 atttcggaag ctatcgagaa gtatcccaaa gcgcttgaaa ttattgaagg gccgctaatg
    11821 gatgggatga acgttgtggg agatttgttt ggcgaaggta aaatgttcct gccccaggtt
    11881 gtaaaaagcg cacgggtaat gaaaaaggca gtggctatat taatgcctgt tattgaagcc
    11941 caaaagggca ataatggcaa catttcttct gctggcaaaa tattgcttgc tactgtaaaa
    12001 ggagatgtac acgatattgg taaaaatatt gttggggtag tgttagcctg caacaattat
    12061 gaggtcatag accttggggt aatggtaccc gcagaaaaaa tattagaagt tgctaaaaaa
    12121 gaaaaggttg acatcattgg tttgagtggt cttataacgc catcattaga agagatggta
    12181 catgtagcca gtgaaatgca acgtcaggga tttgatattc ctttgttaat tggaggtgcg
    12241 acaacttcta aaatacatac agccgtaaaa cttgaaccgg tttacgaaca ccctatcata
    12301 catgtcaaag atgcgtcaaa gagtgttccg gtagtgagca accttttgtc aaaaagcctt
    12361 cataagaatt ttatagaaga agtgcgccgt gaatatgaca atttgcgaac ttcatataat
    12421 ataacttcat cagcggggaa ttatgtcagc cttgaacaag cccgtgctaa taaggtcaaa
    12481 attgattgga atattgaaaa gcctttcaaa cctatgtttg taggtacaaa agtttggaat
    12541 aactacccaa ttgaagatat tcggaaatat atcaactgga tgtttttctt tattgtatgg
    12601 caactgaaag ggaaatatcc tgaaatattg caggatccgg agaaaggcgc tgaagcacgc
    12661 aaacttttta atgatgccaa taaattgttg gatgagttac aatctaagcg tttgattaag
    12721 gccaatgctg tttttggtat atatcctgcc aatgctgttg gcgatgacat tgaagtatat
    12781 acagatgaaa gccgtacaga agttttcaaa gtattcagga atctgcgcaa tcaggctaaa
    12841 caaaatgtaa atgaacctaa tgcctgcctt tcagatttca ttgctcctaa agaatctggt
    12901 attaaagatt atataggcgc ttttgctgtt actgccggca ttggtgttga taagttgata
    12961 gccgaagcta aagaaaacca cgatgattac aaggttatca tgcttgaggc cttggcagac
    13021 cgcttggccg aagcttttac agaactattg catgagaagg tccgcaaaga aatttgggga
    13081 tacgcttcat cagaagcctt atctgtagat gatatgttgc tcgagaagtt taaagggata
    13141 aggccagctc atggttatcc ggcctgtccg gaccatagtg aaaaacaaac cttgtttgaa
    13201 ctgttgcagg caagtaagaa ttcaggaatt agcctgacag aaagtaacat gatgcttcct
    13261 gcggcttctg taagtggttt aatatttgcc caccctcgct ctcaatactt ttttgtggac
    13321 aaaattggta aagatcaagc agaagattat gccaaacgta aaggggtacc ggttggccaa
    13381 gtcgagaaat ggcttaacgc aaacctcaat tataaatagc agtaaaaaat agactttaac
    13441 aacaatactg caactactgc aacattatcc cggctacaaa tcttcctgtt acgccctttc
    13501 ctttgcgtga agaaaagaat ttgtcatcat tacaaatagt gcataagttt gatacttcta
    13561 tattttcagg tttcacgcca gattcgatga gcaaaatccg gttatattcc cacaaattaa
    13621 gaattttgct ttcattgtgt ttttttacca ctttgtcgaa cttgtttttg ttcactttat
    13681 ccaatacttc atctccaact tcataacaac atgggcctgc agatggacca atggctgcaa
    13741 taatatcttc ggcttgggtc ccgtattgct ggttcatttt gttaattgta ttaacaacaa
    13801 tttcgcctac agtacctttc catccggcat gtatagcagc tatagcggcg tttttgctat
    13861 ctaaaagtaa tattgggacg caatcggcca cctgaacaat tagcaataca ttcttttcat
    13921 tggttgtcag ggcatctgta tctgctatgg ctgattccct gtcttttgtc cccaatccac
    13981 aatgtgaagt atcaactttt gcaacattta cactgtgagt ttgccttgga aatacaaacc
    14041 aatcaaacgg tatgccaact gactctgcca aagccatccg gtttttaagg acaacctgag
    14101 tgtcatcttc tacaccaaag ccaagattga agccgtggaa tggaggctgg ctgtaaccgc
    14161 ctattcttgt agaaacaaag tgtttcactt catgaaaata atcaaatatc tcgaactgat
    14221 aaagttgggt atgtttggac gtaacggctc gcatgttaaa tttttgctgc aaagcttaaa
    14281 ataattttta ttcgcaggga ttgtttcaaa aaaataattg aaatttgagt taaataattt
    14341 ttagaatggt tattcataaa cccctatcta tttggtttac aggccttcct ggttctggga
    14401 agtccacttt ggctgatgcc cttacgttag tactaaaggg atacaactat ccaaccatct
    14461 tgctggatgc tgatattgtg cgaaaaggtt tcaacagcga tttggggtat tcattggatg
    14521 accggaaaga aaacatcaga cgtgttgctg agttgtcaaa aattctgata gaacaggaaa
    14581 tttttggcat tcaggctttc atttgtccta ccaacgaatt gcgtaatatg gcccgcgaga
    14641 ttgtaggttt taacaacttt ttcctgatat atctttctac acctcttgaa atttgtgaac
    14701 agcgagaccc taaaggtatg tatgccaaag cccgtgctgg atatatgagt cagtttaccg
    14761 gagttagcca ggagttcgag gtgcctgata cggcgcatat taccctcgat acctccatcc
    14821 ttaccattga agagtgcatc cagagtatca tggagcgcat aggcaggttt aattgatgtt
    14881 gtaacaaaaa aaaacgcttt gcatttcgcg aatgcaaagc gtttagatga atgaagacca
    14941 gtagacgttt taaaacttaa cagtcagtcc tgccatgaaa tttgttccgg cctgaggata
    15001 ataatttacg ctgtagctgc caacgctgcc attggagttg tacatttcgt caaaaatatt
    15061 gtttaccctg aaattaaatt gccagtctgt tcccatgata atggtactat agtttatccc
    15121 accatcggta acggtgtatg cattgagact gcgtattttg ttctcagtat tgtccagata
    15181 ttgcttgcct acatgctttg tattgatgaa cagttccaaa ccttttacag gagttaggta
    15241 tgttattttt cctgcagcaa tcactgatgg agaaaaagat ataattttat ccttataata
    15301 aattactgag tcatcaacac tttcattgta accagttcgg tttttgctaa gggtaacatt
    15361 gccttccagc tttataacgt cgagtagctt tattccgcct gtcatttcaa cccccatcct
    15421 gtagctttta ccaacatttt tgcgaattgg agtcccaaca ttgttcaatt cgcctgtcag
    15481 tacaagctgg tcgcggtatt gcatattgta aaagttgagc tcaaagttga attgatcatc
    15541 gctgtaacgt gtaccaagct ccatgtcgta taatttttca ggctttggct cttctccaac
    15601 ttctgcatca acaaaatcgc tgcgtgaggg ttctctgttt gctacagcaa aggatgtata
    15661 tacaacccat gattttgtag gttggtaaag caaacctacc ttagggttta taaagttgta
    15721 ttgctttttt atactaatag tatcattggc gtactccctg tttgttcccc aggctttgta
    15781 atatatgttt ctatactgaa tatcagcaaa agcagatatt tcattggtta tgctatagct
    15841 tgcttttaag aatgtattaa agtctgtctt tcggccttgg ttgtcgtaaa agtgcattcc
    15901 ttgcatacca tccagaaagt aacgtgccca cattacttgt ccgtaatgct ttgcagggtc
    15961 gtatctgttg taagccccgc cccagataag ggtatatttg ttggcagtat aggttagccc
    16021 ccatataccg ccataaaaat ggttgtcgag ccacaaacgc tggattacat ctgagtatga
    16081 aatggtatca ttaccattta tcccattggg gagtttgaaa taactctgtt cataccagcc
    16141 aggagaaaga tcctggtaat aaccgcgtcc ataagtgtaa tgtagtgcgg cattgaatgt
    16201 cagattgctt gaaaagctat gcattacgtg cagttgaata tggtcttgac tgtaatggtc
    16261 caccatatca tcataataat ggggaactgt gtcattaggc agaaaatatt cgcctaccgg
    16321 gttgtaagta cggcctttgc ttttgatgct gacagagtca atgccatacc aagcctgata
    16381 agtttcttcc tgccccccaa agccaacgag cttaacaata gttttgtcgc tgtaataacc
    16441 agcctgtgca aagtacgatt taaggtcaga cctcgctctg tcaatgtagc cgtcagattt
    16501 gatgcctgtt acttttcctt caaagaaaaa atggtgctta tacaaacctg tacctacatg
    16561 aagcgagttt tttcgggtat tgaatgagcc ataagcgcca gaatactcaa caaatggggt
    16621 ttgcgatatg taatctgtct gaaggttaat actggcacca aaagccccgg agccgtttgt
    16681 tgaagttcct acaccacgct gtatttgtat gctttgcgat gaagttgcaa tatctggtac
    16741 gtcaacccaa tacactccct gtgattcagg gtcgtttagt gggacaccat tgatggttac
    16801 gtttattttg cggccttcga tgccccttat tcgcatcgca gtgtacccaa tgccattgcc
    16861 tgcgtcagat gttgaaacca ctgaagtttg agaagacaat atcattgggg catcctgtcc
    16921 caaggcaagt tgttttatat cttgcttttt gatagtggta aatgtggtcg gggtaaagtt
    16981 ttgagccctg atggccgata ctaccacttc ttcaaagttg gtaacattct cttttaagat
    17041 aatattgcgg gtttggtcag aggtaaagtc aatattctca aaataagtat tatagcccag
    17101 atatacaacc tttacttcct tctggccttt ttcgatattg tctattttaa atgcgccgtt
    17161 ttcgtcggca acaaagcctt tgttatcaac aaccaccgtg gccccggcta aaggttcgtt
    17221 gtgttcattt tttatagtgc ctgtaaatga aaattgtgcc tcgagggcag aaaccaccaa
    17281 caatggtaaa agtaaaaacg taaaaactcg cttcataatt ttttgaagtt taataattta
    17341 acaaaaaatt acagcaatga agtaatggaa aattaaggta agcttcactg cctcttattc
    17401 ccttcgccgg cattacccgg atcaggttca aagggtataa tctcagcctg ttatttaagg
    17461 caccccctta gagacgctgc aaagtaaata aatttatatt tataacacca aaaatttaac
    17521 cttgttaatt ttggcattac atttatattt gccaagccaa attaagatta tggaacagaa
    17581 gcgtaagatt gcatttatta tcaaccccgt gtcgggaact acatcaaagg ctgaaatgcc
    17641 ttcgatgatt gaaagcatca ttgataaaag caaatatgat gtttgtatca agtttaccac
    17701 ttgtgccggc aatggtcatg agcttgcaaa gggctttgtt gctgaaggtt atgatatagt
    17761 agtttcagta ggcggcgatg gcactatgaa cgagattgct tcagcactta cagaaactaa
    17821 tactgccctt ggccttatcc cttgcggttc tggcaacgga cttgcccgtc acttggggat
    17881 tccgcttgat cataagcagg ctatagcctt gtttaacgat tgtaaaatta tcaagatgga
    17941 ctatggcaag gctaatgacg taaagttttt ttgtacttgt ggcgcagggt ttgatgctca
    18001 tatagggcat acctttgcca gtgccgggaa acgaggtttt ttgacctatt taagaagcat
    18061 ttttactgag tttagaaaat ataaacctaa aaagtataag tttaaaaccg aagaaggcaa
    18121 gtttaaaaag aaagcttttt tggtgacatt tgccaatgcc tctcaatatg gcaataatgc
    18181 ttatattgcc cctaatgccg atattcagga cgggcttatt gatgtctgta tcctcgaacc
    18241 attcccaaaa ttcagggctt tgggaattgg gtttaaatta atgagcaaga aaatagacaa
    18301 gtgcaggtat gttcaaatca tgcagaccga taaagttcgt ataaaaacaa agaagaatgt
    18361 tgtttttcat tacgatggcg agccttgcat tatggataga aaggtaaaaa tatcggcagt
    18421 tcagaaaggg cttaacatag cagtattgag taattctcct ttaaactaaa accgtttggc
    18481 tgaagtttat ctgtctttgg gaagtaatat tggcgatcgg ctcgaaaaca tcaatttggc
    18541 tatcgattac atcaatgaaa atattggtga agttatatgt tgttcgtctg tttatgaaac
    18601 tgaggcttgg ggtataaaca gctcagggat gtatttaaat caggttattt tgattaaatc
    18661 aaacataaag cccatatcaa tccttgaaaa gatttgggat attgaatcaa aacttggacg
    18721 agtaagggtt gtagataata agtatgccga caggacaata gatatagata ttttgtttta
    18781 tgacaatcac gtaatacacg gcgattatgt tactatccct catcctatgc ttcaacaccg
    18841 cagatttgtt ctggttccgc tgtgtgatat tgcggccgat tttgtgcatc ctgagttaaa
    18901 taagaaggta gccgatttgt tgcaagtttg taaggatgat cttaaggttc aaaaatttga
    18961 ccccgggttt aattagctat tcatacttca aagtcggaag catataaaaa agtaaactgt
    19021 ccaacattgc ctcatctttg ttcttaattt ttggctcttg tctctttcat tataaaatat
    19081 tacttttgaa acaggcaaaa caatataaac cgcccctcaa tgggtaaact tcaaactgag
    19141 ataaaataca tcaccggcat aggccctaag cgtgccgaga tactgaacaa agacattgaa
    19201 gtatttacca ttaaagactt gctctattat tttccttaca agtatatcga ccgcacaaag
    19261 ttttattcta ttgcagaagt caatgcagac ctgccttata ttcagatcaa aggaaagata
    19321 accaaattta acacaatagg agaaggggct aagaaacgat tgacagcact ttttactgat
    19381 gggacaggct ttatagagct cgtatggttc aaaggtatta aatgggttat gcttcagtat
    19441 aagccagata ttgaatatat tgtttatggt aagcctacac agtttggaca tatcttaaac
    19501 attgcccatc ctgagataga tgaggtgtca aaagcagaac aggttactgc aacagcgtta
    19561 cagcctcaat acaataccag cgagcggatg aaatctaatt ttcttacctc gcgtacaatc
    19621 cataagctga ttgctagttg tttaaaattc actatcaacg agatagagga aacttttcct
    19681 gaatatctga tgaaaacgtg taagctaatc ccgctcaagg aggcacttac gcaaatacat
    19741 tttcccacat cgacagagct gcttactaaa gctgagttca ggttaaagtt cgaagagctt
    19801 tttaccttgc agttaagtct tctcaggcaa aaaaagtaca ggcagcaggt tcatcagggc
    19861 tacatttttt ccaatgtcgg gcattatgtc aacacatttt acaagcattg tctgccattt
    19921 gagcttacca atgcccaaaa acgtgtaatc aaagaaataa gacaagattt aggttcagga
    19981 aaacaaatga accgcttgtt acaaggcgat gttggcagtg gaaaaacact tgtagcgctt
    20041 atgatttcac ttattgccct ggataataac ctgcag
//
LOCUS       DAOSPF010000062        17738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3218_length_17738_cov_6.501499, whole genome shotgun sequence.
ACCESSION   DAOSPF010000062 DAOSPF010000000
VERSION     DAOSPF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3218_length_17738_cov_6.501499"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1616)
                     /locus_tag="PK252_11380"
     CDS             complement(186..1616)
                     /locus_tag="PK252_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOT15351.1"
                     /translation="MKKCMLILLSVVFITFSTSTLSGQKSRLIVLTDIEAEVDDTESL
                     VRLLLYSNQIDIKGLIATTSVWKRTSIAPESIKKLIEAYKKVQPNLLKHETGFPEAQF
                     LLNTVKQGLPKYGMLGVGEGNDSEGSEWIIKVLKEDDERPLWVATWGGVNTLAQALHK
                     ISHTMSKAEAKRLLAKLRIYTISDQDDSGIWIRNNFPELFYIVSPGDDYGSATWSAIN
                     SFIDGVNNEKISNSWLAQHIQQGHGPLGAEYPDVSYGMEGDTPSWLSLIPNGLNVPEH
                     PEYGGWGGRYELYKPDFAKHKKGSSGAPNEPETRAIWTNAIDTYTPYLHNDYGRSIKP
                     DTSTFTDYKVTLWRWRDDFQNDFAARMDWCTKPFSEVNHPPVPVLSHADTLTVKSGEG
                     FGLDASNSTDPDGDNLSFLWFNYPEAGTYKKMIPLNSAENARGAYIVAPEVSKKETVH
                     FILKVTDKGTPQLSRYKRVLVNILPK"
     gene            1825..2673
                     /locus_tag="PK252_11385"
     CDS             1825..2673
                     /locus_tag="PK252_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15352.1"
                     /translation="MLDSENLNKFNEKRNEFKPYGLTCELWTPSVMRKPDRHNEVEIN
                     YFLEGEMTYLVQDKKITIPARRFAIFWGLVPHQIVQYEGNTPYFVCTIPFSQFLEWKL
                     PVLFVDRVLKGEVLIDASEKSAIYDEYLLNNWINDISKSPSIELILLEMHARLYRMAV
                     SNIPAETKEHLSIHTHEISQVEQIAIYIAQNYCNPIKVSDIGKAVGLHPDYANAIFKK
                     AFGCTLTEYIIDERIAHAQRKLVATNESITAIAFECGFNSMSRFNAAFLKVNGCTPRE
                     FRKRFQ"
     gene            3156..4355
                     /locus_tag="PK252_11390"
     CDS             3156..4355
                     /locus_tag="PK252_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT15353.1"
                     /translation="MSEIMENRNDKLNNLFDFAHRLIDGENGKMLIETYQQSINTVNA
                     VDAMQVFDRLLQEGYQVEKVKANVGKIINVFFKALSTDHWTKPDEGHFMYYLMLENRE
                     VEKLIADIRPYIKMFLKSDTFEAHESLQKLRDFISKVKEYELHYIKKENILFPYLEKT
                     FTQHRCLQLMWSFHDDFRRSVKALELLLQPARPDVNAVNTELGKLFFVIMPIIFREEQ
                     IVFPVAIRAIPHKIWNEMLEQSKEIGWCYGVTPKYSGRTSGKEVFNGLVNLGSGFLKP
                     EQLILLLNNLPVDITFVDENDEVCYFSEAKDRIFPRSKAIIGRKVQNCHPPESVHVVT
                     QIVEAFRNGTKDHADFWIQMKNHFIYIRYFAMRNENGQYKGTVEVSQDVTDIRALQGQ
                     QRLLDWK"
     gene            4364..4855
                     /locus_tag="PK252_11395"
     CDS             4364..4855
                     /locus_tag="PK252_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT15354.1"
                     /translation="MIQQVLNEMIAYFGNDVRRINHAIKVYGFATAIGRTENLEAQSV
                     QILETAAILHDIGIPVSEKKYGSCIGKYQELEGPPIARAILEKIGTPANIIDRVCFLI
                     GNHHTYTKIDNIDFQILVEADFLVNIFEDEMYSSVKSIGEKYFKTKTGSQILYTLYPK
                     NNG"
     gene            complement(5039..5386)
                     /locus_tag="PK252_11400"
     CDS             complement(5039..5386)
                     /locus_tag="PK252_11400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOT15355.1"
                     /translation="MTTIKQYDIWIADLEPQRGTETGKVRPVLVIQTNHLNKVHPSTI
                     ICPITTNVQKDSDILRVHISKGKAGLNENCDIMIDQIRAIDNRRLVRKMGDLPKDLIE
                     KVKENIQIILDLE"
     gene            complement(5364..5603)
                     /locus_tag="PK252_11405"
     CDS             complement(5364..5603)
                     /locus_tag="PK252_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15356.1"
                     /translation="MKTLSLRLNDTIFQETEKVVSIVHKTRNKYISEAIDYYNRIQKQ
                     ALLAKRLEKESELVKDESMKVLIEFEALNDNNKAI"
     gene            complement(5812..6483)
                     /locus_tag="PK252_11410"
     CDS             complement(5812..6483)
                     /locus_tag="PK252_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15357.1"
                     /translation="MNILCNNIETTKLILQSVGLLVSSLTLFVAIFINQNFAKRKATE
                     DKIKVVNDLVYCLNTTYIKITYGLSAGNMYGGDLKTWTLFEFSKSNFKLENEYLDFPV
                     VFFNSTQIMDLKGFAYNPLMPIEIADELIKFQNSFCEVITYDEMNLISDKMVILESKI
                     LNDDEIFNSGNKRIDYYQGNAEAYKTYLNFKSSSQSLITTITNWYSDINIKKVNIRAD
                     FNKIK"
     gene            6742..7785
                     /locus_tag="PK252_11415"
     CDS             6742..7785
                     /locus_tag="PK252_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15358.1"
                     /translation="MKKNGFIIAIVITIPLMFSCTKDDEAISPEAVEKWTFYNVNNGL
                     PSNIVNCLTEDDYGNIWIGTNRGLVKYDGTGFRTYSSRSGLPSDTILGLHWDNNDELV
                     VGSANGLGIIKSQSYINILTNSGWECYNSCEDANGNLYTASMYGLLVYSDNQLHLYKL
                     DTGAYDNYITDILCDDYGKIWMAAPFKGLCYVKQNKLTCFPKSKTGMMPYRIIEDKAG
                     NIWMTSVIKDEKVVFYDGYQFVNDTILSEYGDPDYYDHYKAISQDKYGNFWVAIAEKG
                     IFHHAGRMTKGYTTANSPVLMNDVNAILCDSKGNVWFGSNGAGLYCLQNIKPLQFQFG
                     PEYNPYPSNYPNN"
     gene            7803..8882
                     /locus_tag="PK252_11420"
     CDS             7803..8882
                     /locus_tag="PK252_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15359.1"
                     /translation="MQKNRLIWKSGITLAVCMVLCLPSVAQITGLSDQPERYIDRPLT
                     LHSSNMEFSFGYQFGVLSSIYDNKSNSVSIADAGIASSSQAANIQFTYGVTENWQVQA
                     FVNYSYINTSTAMVAQSTGNTVMQTEGIEKISGMFDPELRMAYLLIGSDKKLSLSVGG
                     GLTLPVSAHKPDKPEIKYFAGSLDEYRFVYNYRNGAGSMAYNLNMALKYRFGGTSDNN
                     HSSKLIARLFTDFYTTPGKVSTNDWQYVFDAENPNDFTYQELPVKYKKGDWLVNRLYF
                     DYQAYTIASFMFGISNRLYFHGWKQTADLKVSEYDVSETAFSVGAHIQASPRLRIDES
                     FTLPVMGKSIRTFFTCQLSLVYLIQ"
     gene            8885..9862
                     /locus_tag="PK252_11425"
     CDS             8885..9862
                     /locus_tag="PK252_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15360.1"
                     /translation="MKKLIILYISLVCILPLLAQTERELTVTEIRQQTSVAEPITLQK
                     GYFKVSTALSYVSLAGDWYNNDWEKTPSYSFQSKDFTFPFTFSYGVTNNFQFNISSGY
                     QYSKATHKKEAIDYIGGYSKNINITNNAIGITDIYLGCIYKLMEQNGSLPTIAVGLSL
                     DMPLPGRKPSVDANTYTVDDVPGPQSSAVGIEFYAKKIFYPFALTCDVSYTYRLSTDV
                     YPFLDVFDLTTTKVKPGDLFMANIGFQNMLCDWITLKNEVVYNYIPAAKYDNSLFGYL
                     EIPSVAFSWNPALVFQIRNFRFSQSVTFPFYGKNTGTNPTYAVNVGVKF"
     gene            9867..12647
                     /locus_tag="PK252_11430"
     CDS             9867..12647
                     /locus_tag="PK252_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOT15361.1"
                     /translation="MKLRIVFLFSLLLLLCRCTPPAVLSNQEILQGDEAANRSDYASA
                     VFHYQNFCNINAKLGLYRNLERESEVHRKMGYAYSMMGNFKKALASFRNAASIDSFQQ
                     ANTNLVEDIRGIGNCHLYMGDYKCGLAELKKCIALSNKYEATLKQNQRLIAANVYLSL
                     GNCYAIFSQFDTSNYYMDRAIGIFSKESDNYGLMVSLLKSGINELDLCNYNKAEEKIN
                     RSMKLANDLKLGLSEHYIALGEVNSAKGDYENAFLYKRKALSQADSSANVTQRIACNI
                     SLGDLFTEVGDNKAALAYYNMANSIMEKNDVEKKDAAAMLATRKGDYSSSLQFYAGSG
                     ATFSRGITLLKIGMGFFEKKSFDSCRFYLAEADKLIPLNAKNDAKTLIYIYKAASQIE
                     AGSAQNVQFVLDSARHINQNPDNDWKIFYYQGRAFEADNNDAGAEKAYKQAIDVIETL
                     RGKIRSDELKSSFMDKRIEVYDRLIRLLNKHGRVEESFNYSEKARNRAFLDMLARQKK
                     GISYNSADSVLAMQERKLSTKIIKLREEIYSFSNAKNSTDSTFQVRGVLEKELGQTSA
                     EYETFLTSLKKDNNMFIELIQPSVTDVKQVIAENNDNTILLEYWLSADILYIWCLDNS
                     NISFASVPFDEQKYNMLYEGLAYFSKKSKKTGYYLGELYKLLIAPVRDKLKENSNLVI
                     VPHGMLHFVPFHALIDGSKSYLAEHYCISYAPSASVLRETKNKKRSAGNSLLAMALGN
                     LSLYGMPPLASTDNEVKNIAPLFEKSLVKVENECTVDFFVENAPYFSYLHLATHGVYN
                     SKSPFSSYILFNKTADNDGMLKVSDIFGLQLKSNMVALSACQTGLGNISNSDELVGLS
                     RAFIYAGTPSIVVSLWSVADEPTAKLMTYFYENLKTNPANAALVQAERKLMKTYKEPY
                     FWAPFVLIGDYE"
     gene            complement(12650..15757)
                     /locus_tag="PK252_11435"
     CDS             complement(12650..15757)
                     /locus_tag="PK252_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT15362.1"
                     /translation="MRYLLCFILFFAYTYLLAINNQYFIKNYDPAENNDSLSSDLYCI
                     GQCQGGTLIFGSKSGIYLFDGINTLYYQVSENDYIISCLPIDENKIFVGLSNGFGYLA
                     KSKSGKYIYHQLSSPEDHEKTYKILKYLNIIVFCTKQGLYFFDEKNKFIREIKPVGGF
                     SNESYFHNVFIVDNVLYVRQAGKGLLKLDKDLVEVKNGYFFSDMGIFNIRNYKGKVEI
                     LSVDNGIYLYNKGEIQHIPIPCLSTAKVRGALSLNDGNLCIFTNNNGVFVIDSLYHEY
                     AHFSSQNGLISDKISSAINDIYGNIWTAGENGIHYIAYNSSIMLFNRSFNLPGKIHCV
                     ANKDNILFVASSSGLYSCPANNLSAFVQNTKVKSNVWDIKVFGYQMIIASDNGVYASK
                     NGTIKKISQSPCRFIQTMKNEKFCCIGKKSLLLYNSSFRLVDSLNLEDLQIETITGSA
                     MTVDHDVTTLYISQYSTASNNENILKYKVTENKLEFISTISAPSRIGEVHLINGSVYF
                     STPEVNYMLNHDKFERCSGIFGKYAPIQAYYKDSEMELAVLGDKIGYIKPENTFDYSS
                     FKYIQFQKLFNIERFNGNIYFSTDKGLYTLFSNRKINQKQTPRFTISLIAGKSFFDFN
                     DSAASIDLLYNQNSFNIIFNSTYFYNNIPARFKWRIKENSEPWNDNNNQNSCSFFNVT
                     PGHYTFEIKAVAVNGIESPVKSIYITIEAPWYRKPLAYVVYFILIVAIVIAIVRLYSW
                     RLRRLNAILENKVHERTIALIEKNKEIENQKTQIEKAHHEITDSIHYARRIQSAVLPS
                     NEYIKKIIDNFFIFYRPRDVVSGDFYWVNKVDNTYIFIAADCTGHGVPGAFMSLLGIS
                     LLNEIILENKILSPDIIINNLRERLIKLLNPTNENAELVRDGMDISVCTLNKETKEIH
                     FAGANNKMLIVRTSTNEIVELDADPMPAGYYEVMKPFNLQSINYTEGDIAVLFSDGYK
                     DQFGGPNGKKFLIKRLKELLINVCKEYSSPEKLLEDSFLDWKGNFEQVDDVMVLGFYV
                     NGLKE"
     gene            complement(15773..16501)
                     /locus_tag="PK252_11440"
     CDS             complement(15773..16501)
                     /locus_tag="PK252_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15363.1"
                     /translation="MKTLYTLIFLAITYTSLFPQDRLLVVKVNGIIIAKNLNKELQTG
                     LEIASNEKFDFKAPTATASLINPKSGKIFQLNVKKNIYMPDIEVFEDRGEGDELLFEF
                     FTDTVVFYKRISVTLPQNLYEKFKNQEYILTLFINAKKVSLPFKQTENSFVLQNGDIV
                     NIQPGQYEAQLCSVKKNDKSSSVLSKFLLIIPDNKSFNNECTVIKKVTTDKANLKRML
                     FEFIKINYGKIANSDIEEIMNNEQ"
     gene            16648..16866
                     /locus_tag="PK252_11445"
     CDS             16648..16866
                     /locus_tag="PK252_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15364.1"
                     /translation="MEKVKFFEDNNLSVAVVYFHPCLCLTNHARFMQCGKHKNVFFCM
                     AWPPKRVSLALMVASIASIAFFEAKYYF"
     gene            16935..17330
                     /locus_tag="PK252_11450"
     CDS             16935..17330
                     /locus_tag="PK252_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOT15365.1"
                     /translation="MARTKKNRLIQMAPHFCGFKPQGVQCKKGSEVFINFEEYEALNL
                     CDYELLTQAEAAKMMKISRPTFTRIYESVRRKIAKAFIEGSCIHFEGGNVDISSWYKC
                     EHCKITFTLGDEATKACPLCKKEVITQNV"
     gene            17335..17646
                     /locus_tag="PK252_11455"
     CDS             17335..17646
                     /locus_tag="PK252_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT15366.1"
                     /translation="MKIAIAATENHVKSYVDPHFGRCDWYCIYDTETKKAEFIENIGR
                     NQHEKAGCDAVGFLADKGISLAIAGRFGSKVVEAFRAKDIQMIVPENSRIIKEIINQF
                     K"
BASE COUNT         5312 a         3201 c         3605 g         5620 t
ORIGIN      
        1 taaggcgata agagtttttg agcaactttt ttcgataaaa aagttgcata gagacttagt
       61 atttattgct ttttaaatat taattgacaa tgttattctt tcatcgaatt gtcgttattt
      121 gatgtaacct gttttctata aatatgatgt ccaccgcggc ggacatataa atctaaacaa
      181 atcttttatt tgggcaaaat attcaccaaa acccttttat accttgataa ttgcggggtt
      241 cctttgtcgg taacttttaa aatgaaatga acagtttctt ttttagacac ttcaggagct
      301 acgatatatg cgcctctggc attctctgct gaattgagag gtatcatctt tttgtacgtc
      361 cctgcttcgg ggtagttgaa ccataggaaa ctgaggttat cgccatcagg gtcggtagaa
      421 ttgctggcat ctagtccaaa cccttcgcct gacttgaccg ttaaagtatc tgcatggcta
      481 agtacaggca ccggcgggtg attgacttca ctgaatggtt tggtacacca gtccatgcgg
      541 gctgcgaagt cattctgaaa gtcatcacgc cagcgccaaa gggttacctt gtaatcggta
      601 aaggttgagg tgtctggctt tatcgaacgt ccataatcat tgtggaggta aggagtgtaa
      661 gtgtcaatgg cgttggtcca gatagctctt gtctcgggtt cgttaggagc accagagctt
      721 cctttcttat gttttgcaaa atcaggtttg tacagctcgt aacgaccgcc ccatccacca
      781 tattcaggat gctcaggaac attaagccca ttaggtatca aagatagcca ggatggcgta
      841 tcgccttcca tgccgtaaga tacatcggga tattcagcac caagcgggcc atgcccctgc
      901 tgaatgtgct gagcaagcca cgagttgctt attttttcgt tgtttacccc atctatgaag
      961 ctgtttatgg ccgaccatgt ggcacttccg tagtcgtcgc ccgggctgac aatgtaaaac
     1021 agctcaggga aattgttacg aatccaaatg ccgctgtcat cctggtctga aatggtatat
     1081 atcctgagtt tggccagcaa tcgcttggct tctgcttttg acatggtgtg gcttattttg
     1141 tgtaaagctt gcgccaaagt gttaacacca ccccaagtgg caacccacaa tgggcgttcg
     1201 tcatcttctt tcaatacttt tattatccat tctgagcctt cagagtcgtt tccttcgcca
     1261 acgcccagca ttccgtattt tggtaaacct tgttttactg tattaagtaa aaattgagct
     1321 tctggaaatc cagtctcgtg ttttaacagg tttggctgaa cttttttata tgcttcaatt
     1381 aattttttaa tagactcagg ggctatgctt gtccttttcc atacagaagt tgtagctata
     1441 agtcctttaa tgtctatttg gtttgaatat aagagcagac ggactaaaga ttcggtatcg
     1501 tccacctcgg cctcaatatc ggtcaaaaca atcaaccggc ttttttggcc tgacaaagta
     1561 gaagtagaga atgttataaa aacaactgaa agcaggatta acatgcactt tttcataagt
     1621 atagtaatta gtgtatcaat gtcattaagt taaggctata tttttgataa atcaatcatc
     1681 aaacatcctt cgactccgct cagggtgact gtcagactga gcggagtcga agtctttttc
     1741 agaactaaac gacattgatt tgttttataa attgtaaatt tagcctcgaa tttttgttat
     1801 aactacctgt ttttgtaaat aaatatgttg gattccgaga atttgaataa attcaatgaa
     1861 aaacgcaacg agtttaaacc ttatggcctg acctgcgagt tatggacgcc aagtgtaatg
     1921 cgtaaaccag ataggcataa tgaggtggag attaactatt ttctggaagg cgagatgacc
     1981 tatttggtgc aagacaaaaa gattaccatt ccggcgcggc gttttgcaat tttctgggga
     2041 cttgtgccgc accaaatcgt ccagtatgaa ggaaatacgc cgtactttgt ttgtactatc
     2101 cctttttcac agtttttgga atggaagctt ccggtattat ttgttgacag ggtactgaaa
     2161 ggggaagtgc ttattgatgc ctctgaaaaa tcagcaattt atgatgaata tctgctcaat
     2221 aactggataa atgacataag taaaagtccg tctattgaat taatcttgct ggagatgcat
     2281 gcacgattgt accgaatggc agtaagcaat attcctgctg aaacaaaaga gcatctttct
     2341 atacatacac atgaaataag ccaggtagaa caaatagcta tttatatagc ccaaaattac
     2401 tgcaacccga ttaaagtgtc ggatattggc aaggctgttg gcctgcatcc ggattatgcc
     2461 aatgctattt tcaaaaaagc ctttggctgt accctgacag agtatattat tgacgaacgc
     2521 attgcccatg ctcaacgaaa gcttgtagca actaatgaaa gcattactgc tattgctttt
     2581 gaatgtggtt tcaattcaat gagccgattc aatgctgctt ttctgaaagt gaatggctgt
     2641 actccaagag agttcagaaa aaggtttcag taaaacatat gttagtaata caacggcagt
     2701 tcaatgtaaa agtaattgta agatgcataa atataattat ttatcttctt tccttttttc
     2761 cttgatgaaa aaaggaaacg aaaaaaatca agaaaattta acgctatacg gtttttcatt
     2821 ggttagcttg cgcttcgtag gaacgcaaaa ttttcacgct cttatgaagg gtagtattgg
     2881 cagtagcttt aaatcatagg ctgcgaagca gacaaaaaga gaattcctta tttggctgtg
     2941 aactgtctga gctaatccgc cttcgggcgg aggagtgagt tttcacagcc agtctttttt
     3001 ggtttctttt tttgacgaaa aaaagaaaca atataaatat aattgattat ctgcttgtta
     3061 aaaaatacct tatatcgaat tcacgttaaa atagtcttta gccaataaca aattgtgtat
     3121 tcaattcatt aactttatag taaacactat aggttatgtc agaaatcatg gaaaacagga
     3181 acgataagct gaacaacttg ttcgattttg cacatcgcct cattgatggc gaaaatggca
     3241 aaatgcttat agaaacatac caacagtcaa taaacacggt aaatgccgtg gatgccatgc
     3301 aggtctttga ccgtctttta caggaaggat accaggtaga gaaggttaaa gccaatgtag
     3361 gaaagattat caatgtgttt ttcaaagcgt tgagtactga ccattggaca aaacctgacg
     3421 aaggacattt tatgtattac ctgatgctcg aaaataggga ggtagaaaag ttaattgctg
     3481 acattcgtcc ttatatcaag atgtttctaa agtctgatac ttttgaagcc catgaatcgt
     3541 tgcaaaagct gagagatttc attagcaaag tgaaggaata cgagctgcat tacattaaaa
     3601 aagagaatat tctctttcca taccttgaaa agacgtttac ccagcaccgc tgtttgcagt
     3661 tgatgtggtc gtttcatgat gatttcaggc ggagtgtaaa agcgctggaa ttgcttcttc
     3721 agcctgctcg gcctgatgtg aacgctgtaa acactgagct gggaaagctg ttttttgtta
     3781 tcatgcccat tattttcagg gaagagcaaa tagtctttcc ggtggcaata cgcgctattc
     3841 ctcataaaat atggaacgaa atgcttgagc aaagtaaaga gattggctgg tgttatggtg
     3901 taacgcctaa atattccggg cgtacttccg ggaaagaagt tttcaatggc ttggttaact
     3961 tgggctctgg gtttctgaag cccgaacaac tcatcctgct gcttaacaac ttgccggtgg
     4021 atattacctt tgtggacgaa aacgatgagg tatgttactt ttcagaagca aaagaccgta
     4081 tattccctcg ttctaaggct atcataggcc gtaaggtgca aaactgccat ccacccgaaa
     4141 gtgtgcatgt ggtaacccag atagtagaag ctttccgtaa tggcacaaaa gaccatgccg
     4201 atttttggat acaaatgaaa aaccatttca tctatattcg ttactttgca atgcgtaacg
     4261 aaaatggcca gtataaaggg actgttgaag tgagccagga tgttactgat attagggcat
     4321 tgcagggtca gcaaagattg ttagattgga aataaaaggt tttatgatac agcaagtttt
     4381 aaatgaaatg atagcgtact ttggcaacga tgtgcgccgt ataaaccatg ctattaaagt
     4441 ttatggcttt gctacagcca ttggacgaac cgaaaatctt gaggcacaat ctgtgcagat
     4501 tcttgagact gcagctattt tgcatgatat aggtattccc gtatcggaaa agaaatacgg
     4561 ctcatgcatt ggtaaatatc aggaattaga aggccctcca attgccaggg ctatactgga
     4621 aaagattgga acccctgcca atatcattga ccgggtatgc tttcttatcg ggaaccatca
     4681 tacctacacc aaaatcgata atatcgattt tcaaatcctt gtagaagctg attttctggt
     4741 gaatattttt gaagatgaaa tgtatagcag cgtgaagagt attggcgaaa aatatttcaa
     4801 aaccaagact ggctctcaaa ttctatacac tttatatccg aagaataatg gttaatgaaa
     4861 tttgggcttg gggtaggttt ggttgttaag agtaaatgct taacgcttcc cggctatgcg
     4921 gcgtgccgga tacacggagc caccgccgtc acaaccgata acaaatgtaa tggaaagaac
     4981 ggacttttgc aacttgctaa acccagcatg acgtatgagc tgttgttact atgcgttttt
     5041 attctaaatc cagtattatt tgaatattct ccttaacctt ctcaattaag tctttgggta
     5101 aatctcccat tttcctaacc aatcttctgt tatcaattgc tctaatttga tcaatcataa
     5161 tgtcgcaatt ttcatttaat ccagctttcc ctttgctaat atgaactcgc aatatatcac
     5221 tatctttctg aacattagta gtgattggac atattattgt tgaaggatga actttgttca
     5281 aatggtttgt ctggattacc aaaaccggtc taacttttcc cgtttctgtg cctcgctgtg
     5341 gttccaaatc agctatccaa atgtcatatt gctttattgt tgtcattgag tgcttcgaat
     5401 tcgattaata ctttcattga ttcatccttt actaattctg attctttttc taatctcttt
     5461 gcaagtaatg cctgtttttg aattctattg taataatcta tcgcctcact tatgtatttg
     5521 tttcttgtct tatggactat tgagacaact ttctcagttt cctgaaaaat tgtatcattc
     5581 aatcttaacg ataatgtttt catgtcaaat catattttgg tacaaaaata atcaatctta
     5641 tttgtatatc ataataagat gtcatttttt aatgcaaggc aacgtcttcc atctatgtgg
     5701 tgttcacgaa tctgccaccg actgttttac ccttggtgaa actcattgcg ggatgagacg
     5761 ctcgcacatc ctttaaatcc ggcatgttta tgattcgttg ttgggtatag gttattttat
     5821 cttgttaaaa tccgctctaa tattaacttt ctttatatta atatctgaat accaattagt
     5881 aattgtagtt attaaacttt ggctagaact tttaaagtta agatatgtct tataagcctc
     5941 agcattgcct tgataataat caatcctttt attaccagaa ttaaatattt catcatcatt
     6001 taatatcttc gattccaaaa taaccatttt atcacttatt aaattcatct catcataagt
     6061 aataacctca caaaaagaat tttgaaattt tataagttca tcagcaatct caatcggcat
     6121 taagggatta taggcgaatc ctttcaaatc cataatctga gttgaattaa aaaaaacaac
     6181 tggaaaatca aggtactcat tctccaactt aaaatttgat tttgaaaact caaacaaagt
     6241 ccacgtcttt aaatctccac cgtacatatt accagccgat aatccataag taatttttat
     6301 atatgtcgta tttaaacagt aaactaaatc attcacaact ttaatcttat cttcagttgc
     6361 ttttcttttg gcaaaatttt gatttataaa aatagcaaca aataaagtta agctacttac
     6421 taataatcca actgattgca gaatcaattt ggtagtctca atattattac aaagtatatt
     6481 cattgcttat cgtgttttta acttatactc aacttatgta taacagcctt aatcccattt
     6541 cttccacctt tatcaaaagt aaataatata agctcattat caaaatcata gtccattcaa
     6601 aattgtctgt ctatagctaa aaatgctgtt gacttgaatg agaatattta gtaccttaac
     6661 aagcaaattt gcaatttgtc aaaagacgct agctttgttg gagttgggga tttagagaaa
     6721 aattcaataa acgtaaacag tatgaagaaa aatggtttca ttatagctat agtgattact
     6781 attcccctca tgttttcctg tacaaaagac gatgaggcta ttagtcctga agcagttgaa
     6841 aaatggactt tttataacgt caacaatggt ttgccttcta acattgtcaa ttgcctcacc
     6901 gaagatgatt acgggaatat atggataggt accaacaggg gacttgtaaa atatgatgga
     6961 acaggtttca gaacttattc cagccgctca ggcttgccat cggataccat tctggggctg
     7021 cattgggata ataatgatga actggttgtc gggtctgcca atgggttggg gattataaag
     7081 tcccaaagct atatcaatat attgacaaac agcgggtggg aatgctacaa ctcctgcgaa
     7141 gatgccaatg gaaacctata tactgcctcg atgtatggtt tgttggtgta ttcagacaac
     7201 caactacacc tgtacaagtt agatacgggc gcttatgaca actacattac cgatatcctt
     7261 tgcgatgatt acggtaaaat atggatggct gcgcctttta aaggcttgtg ctatgtgaag
     7321 caaaacaaat tgacctgttt tccaaaaagt aaaaccggga tgatgcctta ccggataata
     7381 gaagataagg ctggcaatat ttggatgacg tctgtcataa aggacgagaa agtggtgttt
     7441 tatgacggct atcaatttgt gaacgatact atcctttcag aatatggcga tcccgattat
     7501 tacgaccatt ataaagcgat aagccaggat aagtacggca atttttgggt ggcgatagct
     7561 gaaaagggta tatttcatca tgcagggagg atgacaaaag gctataccac agccaattcg
     7621 ccagttttga tgaatgacgt taacgctatc ctttgtgaca gcaaaggcaa tgtgtggttt
     7681 ggctcaaatg gtgcaggttt gtattgtttg caaaatatta aaccgctgca attccagttt
     7741 ggccccgaat ataatcccta tccgtccaat tacccgaata attaacccta acagaaccga
     7801 aaatgcaaaa aaatagattg atatggaaat cgggcatcac actggcagtt tgtatggtac
     7861 tttgcctgcc ttctgtagcc caaataacgg ggctttcaga ccagcctgag cgatatatcg
     7921 acaggccgtt gacgcttcac agcagtaaca tggagttttc ttttggctat cagtttgggg
     7981 tgctttcttc tatttatgac aataaaagca atagtgtcag tattgctgat gcgggcattg
     8041 cgtcttccag ccaggccgct aatattcagt ttacctacgg ggttacagaa aactggcagg
     8101 tgcaggcttt tgtcaattat tcgtatatca atacttctac cgctatggtg gctcaatcca
     8161 ccggaaatac ggtaatgcag acagaaggca ttgaaaaaat atcgggaatg tttgatcctg
     8221 aactgcgtat ggcttatctg ttaattggaa gtgataaaaa gctgagtttg tcagtaggag
     8281 gagggctaac gcttcctgtt tcggctcata aacctgacaa acctgaaatt aaatattttg
     8341 caggcagcct ggatgagtac cggtttgtct ataattaccg taatggtgca ggttcaatgg
     8401 cttacaatct caatatggct ttaaaataca gatttggcgg aacttctgac aataatcatt
     8461 cgtcaaagct tatagccagg ctttttacag acttttatac aactccgggc aaggtttcta
     8521 caaatgattg gcagtatgtt tttgacgctg aaaatcccaa tgactttacc tatcaggaat
     8581 tacctgtgaa atacaagaaa ggggattggc tggtgaacag gctgtacttc gattatcagg
     8641 cttacaccat agcatcgttt atgtttggga tttctaaccg cctgtatttt catggatgga
     8701 agcaaactgc cgatttgaag gtgtctgaat acgatgtttc agaaactgca ttttctgttg
     8761 gagcccatat tcaggcatct ccaagattaa gaattgacga gtcgtttacc ttgcctgtga
     8821 tgggaaaaag cattcggaca ttttttacat gccagttgtc gctcgtttat ttaatacaat
     8881 agttatgaag aagctgataa tattatatat ttcgctggtt tgtatattgc ctttattggc
     8941 acagaccgaa cgcgagctta ctgttaccga aatacgccag cagacctctg ttgccgagcc
     9001 tataaccctt caaaaaggtt attttaaggt atctacagcg ctgagttatg tttcattggc
     9061 cggtgattgg tacaataacg attgggaaaa aacgccttca tacagctttc aatcaaaaga
     9121 cttcacgttt ccatttacct tttcgtatgg tgttaccaac aattttcaat tcaatatttc
     9181 gtctggctat cagtacagca aagcaacgca taaaaaagaa gcgattgact atatcggagg
     9241 atattctaaa aatatcaaca taacgaacaa tgccattggt attacagaca tttacctggg
     9301 ttgcatttac aaactgatgg agcaaaacgg ctctcttcct acaatagcag tgggtctttc
     9361 gttggatatg cccctgcccg gcagaaagcc ttctgttgat gcgaatacat atacggttga
     9421 tgatgttccc gggccacaat cgagtgcggt aggcattgag ttttatgcta aaaagatatt
     9481 ttaccctttt gcccttacgt gcgatgtttc atacacatat cggctttcga cagatgttta
     9541 cccgtttttg gacgtgtttg atttaactac aacaaaggtt aagcctggcg atttgtttat
     9601 ggctaatatc gggtttcaga atatgttgtg cgactggata acattgaaaa atgaagtggt
     9661 ttataattat attcctgctg ctaagtacga taattcattg tttggttatt tagaaatacc
     9721 ttctgtggca ttttcatgga acccggcgct tgtttttcag attagaaatt tcaggttcag
     9781 ccagtcggta acctttccct tttatggaaa gaacaccggt actaatccta cgtatgcggt
     9841 aaatgtcgga gttaaatttt aacatcatga agttaagaat agtttttctt ttcagtcttt
     9901 tattgttact ctgccggtgt acccctccgg ccgtgttgtc aaaccaggag attttgcaag
     9961 gcgatgaggc tgcaaaccgc tcggactatg cttctgcggt ttttcattac cagaacttct
    10021 gtaatataaa tgctaagctt ggcttgtatc gcaaccttga acgtgaaagc gaggtgcacc
    10081 gcaagatggg gtatgcttat tccatgatgg gcaattttaa aaaggcttta gcgtcgttca
    10141 gaaatgcagc atcaattgac tcgtttcaac aagccaacac aaatcttgtt gaggatatca
    10201 gggggattgg caattgccat ctgtatatgg gcgattataa atgcgggctt gctgagttga
    10261 aaaagtgtat agcccttagt aataaatacg aagctacatt gaagcaaaac caaaggctta
    10321 ttgctgctaa tgtttatctg tctttgggta actgttatgc catatttagt cagtttgata
    10381 ccagcaatta ctatatggac agggcaattg ggatattttc aaaagaaagt gacaactatg
    10441 gtttgatggt ttccttattg aagtccggga ttaatgagct tgatctttgt aactataata
    10501 aagcagagga aaaaataaac cgttctatga aactggctaa tgatctaaag ttgggacttt
    10561 ctgaacacta catagcactg ggggaggtca atagcgcaaa aggtgattat gaaaatgctt
    10621 ttttgtataa aagaaaggca ttgagccagg ctgattcgtc cgccaatgtt acgcaaagaa
    10681 tagcctgcaa tattagtttg ggcgatttgt ttaccgaagt gggagataac aaggctgctt
    10741 tggcttatta caacatggct aactctatta tggaaaagaa tgatgtagag aaaaaggatg
    10801 ctgcagctat gttggccacg cgaaaaggtg attatagcag cagtttgcag ttttacgctg
    10861 gttctggcgc tacatttagc agaggaataa cgctcttgaa gattggaatg ggcttttttg
    10921 aaaagaaaag tttcgacagt tgtcggtttt acctggcaga agctgataag ctgattcctt
    10981 tgaatgctaa aaatgacgcc aagacgctga tttatattta caaagcggcc tcgcagattg
    11041 aagctggctc ggcacaaaac gttcaatttg tcttggattc tgcaaggcat ataaatcaaa
    11101 atcctgataa cgattggaaa atattttact accagggacg ggcttttgaa gctgataaca
    11161 atgatgccgg agcagaaaag gcctataaac aagcaattga cgttatagag accctgaggg
    11221 gcaagattcg ttcggatgag ctgaaaagct cgtttatgga taagcgtata gaagtgtatg
    11281 acaggcttat caggttgctc aataagcatg gcagggtaga ggagagcttt aactattccg
    11341 aaaaagcgcg taacagagct tttttggaca tgctcgcacg acaaaagaaa ggcatttcgt
    11401 acaatagcgc tgactctgta ttggcaatgc aggagcggaa gcttagtaca aagattataa
    11461 agctcagaga ggagatatac agtttttcta atgctaagaa tagcaccgat tctacctttc
    11521 aggttagggg tgttctggaa aaagagcttg gacaaaccag tgctgaatat gaaactttcc
    11581 tgaccagtct taaaaaggat aacaatatgt ttattgaact tattcagcca tcagtaacag
    11641 atgtaaaaca ggttattgct gaaaacaatg acaatactat acttctggaa tactggctta
    11701 gtgcagatat cctctatatc tggtgtttgg ataacagcaa tatctctttt gcttctgtgc
    11761 cttttgatga gcaaaagtat aatatgcttt atgaaggtct ggcctacttt tcaaaaaagt
    11821 caaaaaagac aggctactat ctgggcgagt tgtacaagtt attaatagct cctgtcaggg
    11881 ataagttaaa ggaaaacagc aacttggtta ttgttccgca tggcatgctt cattttgtgc
    11941 catttcatgc gcttattgac ggcagtaaaa gctacctggc agaacattat tgtatttcgt
    12001 atgctccatc tgccagtgta ctcagggaaa ctaaaaataa aaagcgttct gccgggaatt
    12061 ccctgttagc aatggctttg ggtaacttgt ctctatacgg tatgccaccc ttggcttcta
    12121 cagataatga agtgaaaaat attgcgccat tgtttgaaaa atcgttggta aaggttgaaa
    12181 acgagtgtac agttgatttt tttgtagaga atgcaccgta ttttagttat ctgcatctgg
    12241 caactcacgg ggtttataat agtaaaagcc cattttcatc gtatatcctt tttaataaaa
    12301 cggctgacaa tgatggtatg ctcaaagtga gcgatatatt tggcttacaa cttaaatcaa
    12361 acatggtagc cctgagtgct tgccagaccg ggcttggcaa tatatccaac agcgatgagc
    12421 tggtaggatt aagcagggca tttatatacg cagggacacc gtctattgtg gttagtttgt
    12481 ggagtgtggc cgatgaacca acggcaaagc tgatgaccta cttttatgaa aacctgaaaa
    12541 ccaatccggc taatgcagcc ctagtacaag cagaacgaaa gttgatgaaa acgtacaaag
    12601 aaccttattt ttgggctcca tttgtgctga taggggacta tgaatgacac tattctttca
    12661 acccattcac gtaaaatccc agcaccataa catcatccac ttgttcgaaa ttgcctttcc
    12721 agtcaaggaa agagtcttca agcaatttct caggactgga atactcttta catacgttaa
    12781 tcaaaagctc tttcaaacgc tttatcagga attttttgcc attggggcct ccaaactggt
    12841 ctttgtatcc atcagagaag agtacagcaa tgtctccttc tgtatagttt atactctgaa
    12901 ggttaaaagg tttcattact tcgtaatacc ctgctggcat tgggtctgcg tcaagttcaa
    12961 ctatttcgtt tgtagaagtg cggacgatga gcattttgtt gttggccccg gcaaaatgga
    13021 tttctttagt ttctttattg agggtgcaaa cagatatatc cattccatca cgcaccaatt
    13081 cagcgttttc gttggtcggg ttaaggagct ttattaatct ttcccgcagg ttgtttataa
    13141 ttatgtcagg cgatagaatc ttgttttcga ggattatttc attcaaaagg cttataccca
    13201 aaaggctcat aaaagcacct ggtacgccat gtcctgtaca gtccgcagca ataaatatat
    13261 aagtgttgtc caccttgtta acccagtaaa aatcaccgct taccacatcg cgtggcctgt
    13321 aaaatatgaa gaagttgtcg ataatttttt taatatattc atttgaaggt agtacagcag
    13381 attggatgcg tcgggcgtaa tggatgctgt cggttatttc atggtgagct ttttctattt
    13441 gtgttttctg gttttctatt tctttgtttt tttcaattaa agcaatagtc ctttcgtgta
    13501 ctttgttttc caaaatggca tttagcctgc gcaaacgcca tgaataaagc cttacgatgg
    13561 ctattacaat tgctacaatc aggatgaagt aaacaacgta agctagcggc ttccggtacc
    13621 acggagcttc aatggttatg tatatggact ttaccggact ctctatgcca ttaacagcca
    13681 cagccttaat ctcaaaagtg taatggccgg gagttacatt gaagaaagaa caagagtttt
    13741 ggttgttatt gtcattccaa ggttcgctgt tttcttttat gcgccactta aatcgggcag
    13801 ggatgttgtt gtaaaagtac gtggagttga aaatgatgtt aaaggaattt tggttgtaaa
    13861 gcaaatcaat gcttgcagca gaatcattga agtcgaaaaa gctctttccg gcaattagcg
    13921 aaatagtgaa acgtggagtc tgcttctggt ttatttttcg gtttgagaac agcgtataaa
    13981 gtcctttatc agttgaaaag taaatgttgc cattaaatct ttcaatatta aaaagctttt
    14041 gaaattgaat gtatttaaaa ctgctgtaat caaaagtgtt ttcgggttta atatagccaa
    14101 ttttatcacc taatacagcc agttccatct ccgaatcttt gtaatatgcc tgtattggtg
    14161 catatttccc aaaaattccc gaacatctct caaatttgtc atggtttaac atgtaattga
    14221 cttctggtgt agagaaataa acgcttccat tgattaaatg cacttctcca attctggaag
    14281 gtgcggaaat agtagaaata aactcaagtt tgttttcagt aaccttatat tttaatatat
    14341 tttcgttgtt tgaagctgtg ctgtattgcg aaatatataa ggttgtaaca tcatggtcaa
    14401 cagtcattgc actgccagta atagtttcta tttgcaaatc ttccaggtta agcgaatcca
    14461 ctaatcggaa tgagctgtta taaagcaaca ggcttttttt gccaatgcaa caaaattttt
    14521 catttttcat ggtttgaata aaccggcacg gactctgaga tatcttttta atagtgccat
    14581 ttttgctggc atataccccg ttgtcagaag caataatcat ctgatagcca aagaccttta
    14641 tgtcccaaac attagattta accttggtat tttggacaaa ggctgataag ttattggccg
    14701 gacaggagta cagccctgat gatgaagcta caaacaggat gttgtctttg ttagcaacgc
    14761 aatggatctt tccgggaagg ttgaagctgc ggttaaaaag catgatggaa gagttgtagg
    14821 caatataatg aatgccgttt tcgcctgctg tccatatatt tccatagatg tcatttatgg
    14881 cactgcttat tttatcggat attaagccgt tttgggatga aaagtgtgca tattcatggt
    14941 aaagtgaatc tattacaaac actccgttgt tgttggtaaa gatgcacaaa ttgccatcat
    15001 tcagtgataa tgccccgcgg actttagctg tgctaaggca agggattggt atatgttgta
    15061 tttctccttt attgtaaagg taaataccat tgtccactga taaaatttcg acttttcctt
    15121 tataatttcg gatattaaat atgcccatat cgctgaaaaa gtagccattt ttaacttcta
    15181 ctaagtcctt gtccagtttt agcaggcctt tgccggcctg tcggacataa aggacattgt
    15241 ccacaataaa aacgttatga aaatagcttt cgttggaaaa acctccaaca ggctttattt
    15301 ccctgataaa tttgttcttt tcgtcaaaaa agtaaagtcc ttgtttggta cagaaaacaa
    15361 tgatgttgag gtatttcaga atcttatagg ttttttcatg gtcctcaggt gatgaaagct
    15421 ggtgatatat gtacttaccg gattttgatt tagctaaata gccaaaacca ttggaaagtc
    15481 cgacgaatat tttgttttca tcaataggaa ggcatgagat aatgtaatcg ttttcagaaa
    15541 cctgataata caacgtgttt atgccatcaa aaaggtatat gccactttta gagccgaaaa
    15601 taagagtgcc accttggcat tggcctatgc aatacaaatc agacgaaaga ctgtcgttgt
    15661 tttcggcggg gtcataattt tttataaaat actggttgtt gatggccagt aagtatgtgt
    15721 atgcaaagaa taatatgaaa cagaggagat acctcatggt gcataacatt attcattgtt
    15781 cattgttcat tatctcttca atatcgctgt tggcaatttt cccataatta attttaataa
    15841 attcaaagag catacgcttt aaattagcct tgtctgttgt aaccttctta ataacagtgc
    15901 attcattatt aaagcttttg ttatcaggga ttataagcaa aaacttactt agaacagagg
    15961 agcttttgtc attctttttt actgagcata attgagcttc atactgaccc ggctgtatgt
    16021 ttacgatgtc tccattctgc aatacaaagc tgttttcggt ttgtttgaaa ggtaaggata
    16081 cttttttggc gtttataaaa agagtaagta tatattcctg gtttttaaac ttttcataaa
    16141 ggttttgtgg cagtgttacc gaaatgcgct tataaaacac tacggtgtcc gtaaaaaatt
    16201 caaacaaaag ctcatcgcct tcgcccctgt cttcaaatac ttcaatatcg ggcatataaa
    16261 tgtttttctt tacgttgagc tgaaatattt tgccgctttt ggggtttatc aacgaggccg
    16321 ttgcagtagg ggctttgaaa tcgaacttct catttgatgc aatctcaagc cctgtttgaa
    16381 gttctttgtt caggtttttg gcaattatta ttccgtttac ttttaccacc aacaggcggt
    16441 cttgaggaaa gaggctggta taagtaatgg ccagaaatat taaggtatat aaagttttca
    16501 tagcagaggg tttaagtaat catttataat atctgaaggt ataaattata aaattataaa
    16561 cttaccaaga acagtttttc tatacccaaa tataatgcat tttattggct taatagcctg
    16621 attgttagga aataattacc ttgtcaattg gagaaagtta aattttttga agataataat
    16681 ttatctgtag cagttgttta ttttcatcct tgcttgtgtt taacgaatca tgctcgtttc
    16741 atgcagtgtg gcaaacacaa aaatgtcttc ttttgcatgg cctggcctcc aaaaagagtt
    16801 tcattagcat taatggttgc ttcaattgct tccattgctt tttttgaagc aaaatattat
    16861 ttttaatgaa cataagttca taattatatt tttataatga acttatgttc attatatttg
    16921 caaaaaagaa tgttatggct cgtaccaaaa aaaacaggct gatacagatg gctccgcatt
    16981 tttgtggttt taagcctcag ggagtccaat gtaaaaaagg gtcagaggta tttatcaact
    17041 ttgaagaata cgaagcgctt aacctttgcg actacgagtt gcttacacag gccgaagctg
    17101 ctaagatgat gaaaatatcg cggcctacat ttacccgtat atatgaaagt gtacgtcgga
    17161 aaattgcaaa agcctttatt gaagggtcat gtattcattt cgaaggtgga aatgtagata
    17221 tttccagttg gtacaaatgc gaacattgta aaataacctt tacgctgggc gatgaagcca
    17281 caaaggcttg tccgctttgt aaaaaggaag taataaccca aaatgtctga agccatgaaa
    17341 atagctatag ctgccaccga aaaccacgtt aaatcgtacg tcgatccaca ttttggccgt
    17401 tgcgattggt attgtattta cgatacagaa acaaagaaag ctgaatttat tgaaaatatt
    17461 gggcgaaacc aacatgaaaa agctggttgt gatgctgttg ggtttttagc agataaaggt
    17521 atcagtttgg caattgccgg gcgttttgga tcgaaggtag tagaagcctt cagggccaag
    17581 gatatccaga tgattgtccc tgaaaacagc cggattataa aagaaattat taatcaattc
    17641 aagtaaaata tgaaaatagc agtacctaca agagacaact ttgttgacag ccattttggc
    17701 cattgcgaag cttatactgt ttttaccatt aatgaaaa
//
LOCUS       DAOSPF010000063        17585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3249_length_17585_cov_6.449914, whole genome shotgun sequence.
ACCESSION   DAOSPF010000063 DAOSPF010000000
VERSION     DAOSPF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_17585_cov_6.449914"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="PK252_11460"
     CDS             <1..2112
                     /locus_tag="PK252_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT15367.1"
                     /translation="IIIMNLKFCFSLFLIAFVSLSINPDIKGATPVQQAYNISFSSVK
                     ETSFTVKWLRGSGSKCVVFVKQTSSGTASPVNKTTYKASTVFGSGSQIGTSKWYCVYN
                     GTGTSVTITGLTVGKTYRVMVCEYNGTNGSQEYLTTSASKNPANQAPDYTWPTIQAKN
                     LTFSNISSTSITASWTRGNGSQCILFVKQTNSGTASPVNNTTYSANNTFGSGSQIGTS
                     GWYCLYKGTGKSVVVNGLTPGTTYQFMTCEFNGSNGFEKYLTSTTSGNPAVQTTITAT
                     PTIQASKVSFANVTANSFNLSWTRGNGSNCAVFVTTNTTSSLSLTNNTTYVPNNVYGL
                     GTQVGTSGWYCVYTGSGTNINVTGLKPGVTYKAMVCEFNGNASNEKYLVSTTSNNPNT
                     RKTIAITTWDGTSWDNGKPNPETNAVVNADLKLNENIVCNDLIINAGKNLTINAGYCL
                     TVQNNITNNGYLILKSPLSEASSAMLINYGDITNNGTMTAERYITPGTSLSSNKVWHN
                     MSSPVGSFKVEKSFFGDYVSQYVESTNGWKALVSGNLITPTTGYIVKTIQTGGKTINF
                     TGTFNTGNQSVALTNTGADANHGYNLVGNPYPSAIDWEASSGWTKTNISRTIWIWNPY
                     TQVYSTWDGTVGTNGGSRYIPAMQGFIVKVNEGYTTGALAMNNNVRVYNTKSYSKKAK
                     TEPELIRLKTSGNELSDELIV"
     gene            2143..2706
                     /locus_tag="PK252_11465"
     CDS             2143..2706
                     /locus_tag="PK252_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15368.1"
                     /translation="MFSTNKYVPQISAANAAGDYTIVKLAENTTDTTIQLSFRCDTSG
                     KYTISAEDMSFNNGTSTIYLIDKQEGMTIKLESGTSYTFYHDTKNEVKRFALMLSATD
                     ETNTTNINNQSESEINIWSYNKKIMANITENGKFDLEVYNLSGVLVAQKTINQKGISS
                     LQLSQKGVYIVKLKNTEITKTKKIIVK"
     gene            2706..2954
                     /locus_tag="PK252_11470"
     CDS             2706..2954
                     /locus_tag="PK252_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15369.1"
                     /translation="MKNKLLQIIAIALLFFTNVVAFAQSSPTPVQPANGPSDPGGGDP
                     AIGTPLDSNAIFVLIFAGGLLLLLINRKKVLEFVKSQK"
     gene            3119..3910
                     /locus_tag="PK252_11475"
     CDS             3119..3910
                     /locus_tag="PK252_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15370.1"
                     /translation="MTYDKKIILFSVNSFFAFLSSFLLLHILSQIVTIIVAHANSIPT
                     RFNYFNVIFPISDDSNLWTQNSVVSIYLSGPLFCLLLGLAFRRYLTISIDNPNRNTKL
                     FFIWGYTHAMNCFFGGLIAGIPLLKGIGYVPIWLYFSNTALIITIVFSGVLLLLNGFF
                     LRNIFSSLSYAEICWKKPFLFKFMIIYAPYLLAFALFFILRFPDNSIYQQALMVTLLL
                     QLFFIMSNSYIHEWDDEVKVSIAVSWKLILLCLSLFWIIFIWSKM"
     gene            3910..4992
                     /locus_tag="PK252_11480"
     CDS             3910..4992
                     /locus_tag="PK252_11480"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HOT15371.1"
                     /translation="MDYLKKILAIIKKNRPLSTYLIAILTVFIFLFIVKKPLQKLFLD
                     IHLTQTYYNTLINISASILHFLGFNWQETEHGLLVKSLSGDYIIHNRLMSLRYAFFII
                     TGGILLSSGLLRKIKLIALSLLLLQIFNITRIAVMLFFVSSYKTYEYRLIREIFELIL
                     HLGILAYIIYWFKNNWRLKRLLIGAYRIKKKTIKTFFVKLFLATFIFSIILFFIKTNT
                     LGVANILSTGILKGAQGILSLMGYSSNVDSATCILQGPNCRLHLSYLCLGINLMFVFA
                     AFIIFTNGLFWQKLIFISAGIVFIYFFNILRFVFLFIFVTNNQGNDAAILDTHDLYSY
                     VIYVLTFITWGIWLKYFSKPQQNPIK"
     gene            4997..6145
                     /locus_tag="PK252_11485"
     CDS             4997..6145
                     /locus_tag="PK252_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOT15372.1"
                     /translation="MPTSKLNLKKIIPVILILLAINNLIAIARSIEEKQSILFLIDIS
                     GSMKGPKIDSVKSATKRIVEMISPCGAEISVMGFCGEKDNPIAYRLNFTSDKDKICNF
                     IDSLRPNGNTPLGAALINATYYIKANKSPQSVKQTIILLGDGRSDDNVAEALKELTAK
                     KALVQCECIGFCVQNEKYAEQQLMDIAQTTHGEYYVSSAATNVFKAFLKTSIKAFIKD
                     IPVEVRQIKKGFNFKEIAVADFKTIAEQAWVLDSIQINALPEIYNITSIITNENIQDT
                     MPKALVFDANRKVSLFINNGTQADAYKKWIDGRYTFDRNTITINLPEHFIKMVVKKVD
                     NKALVLCVNKYRNLVDSMIEGGELCDCSNKPSTEKPTILVYFSRPGCK"
     gene            complement(6151..7173)
                     /locus_tag="PK252_11490"
     CDS             complement(6151..7173)
                     /locus_tag="PK252_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT15373.1"
                     /translation="MYSFRDDYSEGCHPGILEALSTTNMSQQDGYGDDEYSLKAKQLI
                     KKRIGNENADIFFVSGGTQANLSVIASILRPHEAVVAVRSGHIAVHEAGAIESTGHKV
                     CVVDSSDGKIVSHQIKSLLEEHKDVPHMVKPRLVYISNSTEVGTFYTKAELADLYDFC
                     KKNGMYLYLDGARIGMALSAKDNDLQFADLAVLTDVFYIGGTKNGALLGEAIVINNNV
                     LKADFAFHLKQRGALLAKGRVLGIQFYELFKDNLYLELAQWANDMAQKLAKAIKNAGY
                     QFYSQPATNQLFPIFPNELILKLEKEFSFYRWCRVDDKHTVIRLVTSWATREEAVEEF
                     AKSLKG"
     gene            7317..8132
                     /gene="cdaA"
                     /locus_tag="PK252_11495"
     CDS             7317..8132
                     /gene="cdaA"
                     /locus_tag="PK252_11495"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT15374.1"
                     /translation="MFFIKVQVLDIIDITIVAYLMYRFYILIRGTVAINIFITIFSIY
                     LFWLVAKALDMQLLGTIIGQIIGVGVIALIIVFHQEIRRFMLIIGTRYMSRTFSLKNL
                     VFASKGQQQRIQIKEIARACVNLSKAKIGGLIVIERNSALHFYADTGVILNANTSASL
                     IESIFFKNNPLHDGAIIIIGEKIFAARCMLPMSQNVQLPEHLGMRHRAGVSMSEHTDA
                     LIIIISEETGNVSIAEFGKLTQNITLKSLLKTLEKEFHGEIKQQNRVEPIKTY"
     gene            complement(8129..8626)
                     /locus_tag="PK252_11500"
     CDS             complement(8129..8626)
                     /locus_tag="PK252_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15375.1"
                     /translation="MNTQNTILNPFKTAMATIVMLFLSFYAIAQDCYFYHEYQCKFPN
                     TTYYLSGQSRSALFTYGMTSEFKLVTFSNEDYHISICYQFKIKNIRFRLIEDNEDKTV
                     LFDNAEANYKTEVSFTNTEARRIIVEVSVPEVANAKDKDEKQCVGVLVHSRKTLREPK
                     STLGF"
     gene            complement(8652..9533)
                     /locus_tag="PK252_11505"
     CDS             complement(8652..9533)
                     /locus_tag="PK252_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15376.1"
                     /translation="MKLKITFGILALTLAMGCRNASNSSEQAISTDSLPVNQTDKKTQ
                     SVYYRFPTPDELFTFIKKENIKYNPEILNPVSNISRYVNSKAQTIALGVYLADLSYIT
                     LFESYNYSLEYYKAIHSLSEKVKITSAYDLSIAKRIEKNLLNLDSLKQISTESYAGIV
                     EFLIMNNREKTLALIAAGAYIECFYLTFKLTDKNEAHNPLKTRVADFKYAFENLYSYL
                     TIYSDDKQVAQVAEQLKGVHELFSSIKSSKKTKTKVRQDKEGNLIFEGGSKLEITAEQ
                     YSQLKEMIFAVRKSFIE"
     gene            9780..10478
                     /locus_tag="PK252_11510"
     CDS             9780..10478
                     /locus_tag="PK252_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT15377.1"
                     /translation="MNKTKTTYQANPRHYVAVDSIVFGFDGEDLKILLIKRKIEPLKG
                     EWSLMGGFVQEKQSIDQAAEIVLKNLTGLDNVYLEQLSTFGEVQRDPIDRVISVAYYA
                     LIKLNEYDRQIVHNNGGEWFSISNYPKLILDHEEMVQTAISKLRRKCRTQPIGFELLP
                     EKFTIPQLQSLYEAIWMQKLDKRNFRKKFLSMNLLDKLEEKDKENSKKGAFYYKFNKE
                     KYEQLTSRGFIFEI"
     gene            10520..12250
                     /locus_tag="PK252_11515"
     CDS             10520..12250
                     /locus_tag="PK252_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOT15378.1"
                     /translation="MEMNFDLAWIDYAVMAVYFIFVLGIGWALKRFMKDANAFLEAGR
                     SMPAWVAGLAFISTNLGALEVMGMTGSGAKYGLLTAHFYWVGAIPAMVFLAIFMMPFY
                     YGSKARSVPEYLKLRFDEKTRGMNAFMFAAMTILASGISMYALAILTEKVLGWNFHVS
                     LIVSAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPIVFLSLKQLGGWSGLEQALAP
                     VAIQNNFAADAYTTTWKYTGSGGSNPLGVPWYGIFMGLGFVLSFGYWCTNFLIVQRAM
                     SAESTTAARNTPLIGALPKMFIPFLIIVPGIAALALMNLPGSGFSLPVNAAGQPIYDY
                     TIIALLKQYLPAGVLGLGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRPATGNKE
                     ADDKHYLFVGRMATVFGVLVSVAAAYLASMFGNIMDFLQTIFSMINAPLFAVIFLGMF
                     WKRSTGHAAFAGLIAGLTIAFLHHGLTFPAGADTLVKGGWIAKLHEYPVEMAQNFWTA
                     IYACFTSALVTVVLSLMTKQQKTDEDLKGLVYSLTPKLQESHVVWYKRSSSLAILVIA
                     IALLLTILFW"
     gene            12270..12476
                     /locus_tag="PK252_11520"
     CDS             12270..12476
                     /locus_tag="PK252_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15379.1"
                     /translation="MGLDIKYPIGLMFTILGIMITIYGFATINDTTLYQKSLGININL
                     WSGISMLVFGIVMLVLSKKNKKEE"
     gene            complement(12956..13933)
                     /locus_tag="PK252_11525"
     CDS             complement(12956..13933)
                     /locus_tag="PK252_11525"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT15380.1"
                     /translation="MAKNIAVVAGGNSSEYVISVQSGAMLAEEVSKAGYNMFLVKLKG
                     AEWKAKLPDGRLVDVDKNDFSISIDAQKVKFDCALIAIHGTPGEDGMLQGYFDCLQVP
                     YTSCGVLASALTFDKYACKLFLNNFGINTAKGILISKNEKWDVPHITSVVGFPCIVKP
                     NASGSSFGVTKVDEENQLLGAIEHALTESNEVLIEQFISGTEVTNGVYVIDKEELVFP
                     VTEIVSKTSFFDFEAKYNAALSEEITPARISDEITRKVQQTSLKIYKALNCKGIVRID
                     YIINDGAPYFLEVNTVPGMSPNSIIPKQIKTMGLTVAEVYKKIIDSICR"
     gene            complement(13982..14515)
                     /locus_tag="PK252_11530"
     CDS             complement(13982..14515)
                     /locus_tag="PK252_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT15381.1"
                     /translation="MLSSCGYNSMVQKEEAVSSQWAQVQNVYQRRADLIPNLVNTVKG
                     YADFEKSTLTAVIEARAKATSVNIDPAKLNAESFKQFQQAQDGLQSALSRLMVVVEKY
                     PELKANQNFLELQSQLEGTENRITIERMKFNELTKDYNAYIRSFPQLLFAKMFGFSSK
                     PYFEATAGADKAPEVKF"
     gene            14613..15269
                     /gene="mnmD"
                     /locus_tag="PK252_11535"
     CDS             14613..15269
                     /gene="mnmD"
                     /locus_tag="PK252_11535"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOT15382.1"
                     /translation="MQRILKTSEDGSSTVYSEQTGETYHSIHGAYTESMHVFINNGLS
                     LVKSNKINVLEIGFGTGLNAWLSYIYAINKGLEISYDAYELYPLPFSLVNLLTYKDIY
                     PEKQEKFELIHSVPWETTAKIDHNFYLSKKCSDITVCTFNGIYDMVYFDAFSPDSQPE
                     MWTGKVFEKIFQDMNHEGILTTYCVKGIVKRALKEVGFKIEVLPGPPGKRHMLRAWKM
                     "
     gene            complement(15740..17395)
                     /locus_tag="PK252_11540"
     CDS             complement(15740..17395)
                     /locus_tag="PK252_11540"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOT15383.1"
                     /translation="MNFIQSLFSGTGTATTILFISFAAFVGILIGKLEFKKVKLGIAG
                     VLFSGLIFAHFGAQADPHVLHFVREFGLILFVYAIGIDVGPRFFSTFRNDGLKLNLFA
                     AGIVVTGFSIAYFIHAVLGVSPAVITGIMSGAVTNTPGLGAAQQALNEVGQADAAAVT
                     GMGYAVAYPFGVLGIIITMILVRFIFSIKVNKEVEDYNGQYSNSQKKLESVEIYISNP
                     NMFGKEIKYIKKIVDKELVISRIERDGKFLVAKENEKIQQGDVIYGVSAINLIENLRM
                     KMGEVKIGSKREISGDLAMFHVLVTNRKLTGKTIEQIGIYRRYEANITRIFRAGMEIL
                     PTHDTTVELGDTVRVVGKRDLLPEIREELGNSVKELAHPNTIPIFLGIFLGIILGSVP
                     LFIPGLPAPAKLGMAGGPLLIAIILGYKGRIKNLDFYMTPGANMMLRELGIILFLACV
                     GLSSGSEFVKTLVNGGYIWLLYGAAITFIPIFLASVIARLMKMNYLKICGIVSGAMTD
                     PPALEFANSLAPVHAQSTAYATVYPLTMFLRILLAQTLILITL"
BASE COUNT         5686 a         3364 c         3062 g         5473 t
ORIGIN      
        1 ataataatta tgaatttgaa attttgcttc tctttatttt taattgcttt tgtttcatta
       61 agcattaacc ctgacattaa aggggcaact cctgttcaac aagcatataa tatttctttt
      121 tcttctgtaa aggaaacaag ctttaccgta aaatggctca ggggtagcgg tagcaagtgt
      181 gtggtgtttg taaaacaaac ctcttcaggt acagcctctc cggtaaataa aactacttac
      241 aaagccagta ccgtgtttgg ttctggctcg caaattggta cctcaaaatg gtattgtgta
      301 tataatggta caggtacctc tgttaccatt accgggctta ccgttggaaa gacatatcgg
      361 gtcatggttt gtgaatataa tggcacaaat ggttcacaag aatatcttac tacttcggcc
      421 tccaaaaacc ctgctaacca agcccctgat tatacctggc ctacaattca ggcaaaaaac
      481 ctcacgttct caaacatcag ttcaacaagt attacggcaa gctggactcg cggcaatgga
      541 agccaatgta tattatttgt aaaacaaaca aactcaggta ctgcctcgcc tgtaaataat
      601 acaacttact cggctaataa tacttttggc tcaggctctc aaataggtac ttctggatgg
      661 tattgtttat ataaaggaac cggaaaaagc gttgttgtca atggattaac ccctggaacg
      721 acttatcagt ttatgacatg cgaattcaat ggctcaaatg gatttgaaaa atacttaact
      781 tcaacaacct ctggtaaccc tgctgttcaa acaacaataa cagcaacccc aacaatacaa
      841 gcttctaaag tttcgtttgc taacgtaacg gccaatagct ttaacctttc atggacaaga
      901 ggcaacggaa gcaactgtgc tgtatttgtt acgactaata ctacctcgtc actttctctg
      961 acaaacaaca caacctatgt acccaataat gtttatggac ttgggacaca agtcgggact
     1021 tcgggctggt attgtgttta tacaggaagc gggacaaaca ttaacgtaac cgggctaaaa
     1081 cctggtgtta cctacaaagc catggtttgc gaattcaatg gaaatgcgtc aaatgaaaaa
     1141 tatttggttt caactacctc aaacaacccc aatacaagaa agacaatagc cataacaaca
     1201 tgggatggaa catcctggga caatggcaaa cctaatcctg aaaccaatgc tgttgttaat
     1261 gcagacctca agctaaatga aaacattgta tgtaacgact taattattaa tgcgggcaaa
     1321 aatcttacta taaatgccgg atattgcctt acagtacaaa acaacatcac caacaacggt
     1381 tatttaatcc tcaaatctcc cttgagcgaa gcttcatctg cgatgttgat caattatggc
     1441 gatatcacca ataatggaac aatgactgcc gaaagatata ttactccggg gacaagtctt
     1501 tcttcaaaca aagtatggca caacatgagt tctcctgtgg gaagctttaa agttgaaaaa
     1561 tcgttctttg gcgattatgt atcacagtat gttgaaagta caaatggctg gaaagcctta
     1621 gtatcgggaa acctgattac cccaacaaca ggctatatag taaaaaccat acaaactggc
     1681 ggaaaaacca tcaattttac aggcaccttt aatactggca accaaagcgt agcattgacc
     1741 aataccggcg ctgatgctaa tcatggatat aaccttgttg gaaatccata tccttctgcc
     1801 attgattggg aagcctcctc gggttggaca aaaacaaata tctcccgtac catttggatt
     1861 tggaacccat atacgcaggt atattcaaca tgggacggta ctgttggcac taatggaggc
     1921 agcagatata tacctgctat gcaaggcttt atcgtaaaag tcaatgaagg atataccacc
     1981 ggggctttag ccatgaacaa caatgtaaga gtttacaata caaaatcgta tagcaaaaaa
     2041 gctaaaactg agcctgaatt gataagatta aaaacctcag gcaatgaact ttctgacgaa
     2101 cttattgttt aaaagtcaga ggttaatggt aataccgaaa aaatgttcag caccaacaaa
     2161 tatgtaccac aaattagtgc tgctaacgct gcaggagatt acactattgt aaaattggca
     2221 gagaatacaa ctgacacaac aatacaatta agtttcaggt gtgataccag tggtaaatat
     2281 actattagcg ctgaagatat gtcatttaac aatggcactt caactattta cctcattgac
     2341 aaacaggaag gcatgacaat aaaattagaa tctggaacaa gttatacctt ttatcatgac
     2401 acaaaaaatg aagtgaaaag gtttgctttg atgctttctg caacagatga aacgaataca
     2461 acaaatatta acaatcagtc tgaatcagaa atcaatattt ggtcgtataa caaaaaaata
     2521 atggcaaata ttacagaaaa tggaaaattt gacttagaag tatataatct ttccggggtt
     2581 ttggtagccc aaaaaaccat caaccagaaa ggcattagca gtttacaact aagtcaaaaa
     2641 ggtgtatata ttgtaaaatt aaagaatact gaaattacca aaacaaaaaa aataattgta
     2701 aaataatgaa gaataagctt ttacagataa tcgcaatagc actacttttt tttacaaacg
     2761 tagttgcatt tgcacaaagc agcccaaccc cggtacaacc tgcaaacggc ccatcagatc
     2821 caggaggcgg agatccggca ataggcacac ctttagacag taatgctatt tttgttttaa
     2881 tatttgcagg aggcttgtta ttactgctaa taaacagaaa aaaagtatta gaatttgtaa
     2941 aaagccaaaa gtaaaaaaat ttacatacta tagaatcaat ctattttgta acagttactc
     3001 aatagattga tttttttgtt atcaatacaa aacaattaaa aaccaacaac ctgtatttga
     3061 acaaattaga acactttcat tttctgactc tatggaagtc aaagacaaaa atataagaat
     3121 gacttacgac aaaaaaatca tactattttc tgtcaattcg ttttttgcat ttttatcttc
     3181 gtttttgctt ttgcatattt tatcccaaat cgttacaatc attgtagccc atgctaactc
     3241 aatacctaca cgctttaatt atttcaatgt catattcccc atctctgacg attcaaatct
     3301 gtggacacag aactcagttg tttcaatata tttatcaggc cctttatttt gtttgttatt
     3361 aggccttgcc ttcaggaggt atttaacaat ttctattgat aaccccaacc gaaacaccaa
     3421 gctattcttt atctggggat acactcatgc catgaattgc ttctttggcg gcctaattgc
     3481 aggtattcct ttattaaaag gcattggcta tgtccccatt tggctatact tctccaatac
     3541 ggctttaatt ataactattg tattttccgg agttttattg ttactgaacg gattctttct
     3601 gcgaaacatc ttttcatctc tttcttatgc tgaaatatgc tggaaaaagc cttttctttt
     3661 caaattcatg atcatatatg ccccttattt attggcattt gctttattct ttatactcag
     3721 gttcccagat aacagcatat atcagcaagc tttaatggta accttgctac tccagttatt
     3781 ttttatcatg tcaaacagct atatacacga atgggatgat gaagttaagg tttctattgc
     3841 agtctcatgg aaactgattt tattatgctt atctttattt tggataatat ttatatggag
     3901 caaaatgtaa tggattatct aaaaaaaata ttggctataa ttaaaaagaa tcggccttta
     3961 tccacttatc ttatagctat tttgactgtt tttatatttc tttttatcgt aaaaaaacca
     4021 ttacaaaagc tatttcttga tattcatctc acacaaacat actacaacac ccttattaac
     4081 atttcagcct ctatactgca ttttttgggg tttaattggc aagaaaccga acatggctta
     4141 cttgttaaat ctttatctgg cgattatatt atacataaca ggctaatgtc cctcaggtat
     4201 gcttttttta taattacggg cggaattttg ctatcttcag gtttgctgcg caaaataaag
     4261 ttaatagctt tatcgctgct attgctgcaa atttttaata tcacccgaat agctgtaatg
     4321 ttattctttg tatcatcata caaaacatac gaatataggc ttattagaga gatatttgag
     4381 ctcattctgc atttaggaat tttagcctac attatttatt ggtttaaaaa taactggcgt
     4441 ttaaaaaggt tgttaatcgg agcgtataga ataaaaaaga aaacaatcaa aacattcttt
     4501 gttaaattat ttttagccac atttatattt agtattattc ttttcttcat caaaactaat
     4561 acccttggtg tggccaacat attgtcaaca ggaattctga aaggggccca gggaatatta
     4621 tctttaatgg gttattcatc caatgttgat agcgctacat gtattttgca aggccccaac
     4681 tgccggcttc atttgtcgta tttatgttta ggtatcaacc tgatgtttgt atttgccgct
     4741 tttataattt ttaccaatgg acttttttgg caaaagctaa tttttatttc tgctggaata
     4801 gtgtttatct atttttttaa cattttgcgt tttgtatttc tatttatttt tgtcactaat
     4861 aatcagggca atgatgccgc aattcttgac acccatgatt tatattctta cgttatatat
     4921 gttttaacct ttattacatg gggcatctgg ctcaaatatt ttagcaagcc ccaacaaaac
     4981 ccaataaaat aaaacaatgc caacatcaaa attgaactta aaaaaaataa taccagtaat
     5041 acttatttta ttggccatta acaacttaat agctatagcc cgttcaattg aagaaaaaca
     5101 aagtatcctt ttcctgatag atatttcggg cagcatgaaa ggccctaaaa tagactcggt
     5161 aaaaagcgcc acaaagcgta ttgtagaaat gatatcgcca tgcggggctg aaatatctgt
     5221 gatgggcttc tgtggcgaga aagacaaccc tattgcttac agactaaact ttacttctga
     5281 caaagataaa atatgcaact tcattgacag cctccggccc aacggaaata cccctcttgg
     5341 ggctgcttta ataaatgcta cttactacat aaaagccaat aaaagccctc aatctgttaa
     5401 acaaaccatc attctgcttg gcgatggccg cagtgatgat aatgtagctg aagctttgaa
     5461 ggaattgaca gctaaaaaag cattagtaca gtgcgaatgt attggttttt gtgttcaaaa
     5521 cgaaaaatat gccgagcaac agcttatgga tatagcccaa actacacatg gcgaatatta
     5581 tgtatcttcg gcagctacaa atgtctttaa agcattttta aaaaccagta ttaaagcctt
     5641 tatcaaagat atacctgtcg aagtcaggca gataaaaaaa ggttttaact ttaaagaaat
     5701 agccgttgca gattttaaga ccatagcaga acaggcatgg gtgcttgact ctattcagat
     5761 aaacgctttg cctgagattt acaacattac cagcattatt accaatgaaa acatccagga
     5821 tactatgccc aaagctttag tttttgatgc taaccgaaaa gtttctcttt tcattaataa
     5881 tggaacacag gctgatgcct ataaaaagtg gatagatggc agatatacct ttgatcgcaa
     5941 caccattacc atcaaccttc ctgagcattt tatcaagatg gtggtaaaaa aagtggacaa
     6001 taaagcactt gtcctctgtg tcaacaaata ccgaaacctt gttgacagta tgattgaagg
     6061 tggcgaactt tgcgattgtt caaataagcc ttcaaccgaa aaacctacca ttctggttta
     6121 cttctcaaga ccgggctgta aataacagta ttatcctttt aaagatttag caaactcttc
     6181 aacggcttct tcccgcgttg cccatgaggt aacaagcctg atgacggtat gcttatcatc
     6241 tacccgacac caacggtaaa aactaaattc tttttctaac ttcaaaatca actcattggg
     6301 aaatatagga aacaactgat tggtagctgg ttgcgaataa aattgatatc cggcattttt
     6361 aatggccttt gccaacttct gtgccatatc attggcccac tgtgcaagtt caaggtaaag
     6421 attgtctttg aatagttcgt aaaactggat acccaacacc cttccttttg ccaacaaggc
     6481 tccacgctgc ttgaggtgaa aagcaaaatc tgctttgagc acattattat tgataacaat
     6541 ggcttcgccc agtaaagccc cgtttttggt acctcctata taaaacacat ccgtcaatac
     6601 agccaaatca gcaaattgta aatcattatc ctttgcagat aaagccattc ctattcgagc
     6661 cccatcaagg taaagataca tcccgttttt cttgcaaaaa tcataaagat cagccaactc
     6721 agccttagta taaaaggttc ccacctcggt agaatttgaa atataaacca gccgtggctt
     6781 gaccatgtga ggtacatcct tatgctcctc taataaagac tttatttgat gagaaacaat
     6841 tttcccatcg ctactatcta caacgcatac tttatgaccg gtagattcaa tggctcccgc
     6901 ttcgtgtacg gcaatatgac cagaacgcac tgcaaccaca gcttcatgag gcctcaagat
     6961 agaggcaata acagacaaat tggcttgtgt acctcccgaa acaaagaaaa tgtcagcatt
     7021 ctcattccca atccgtttct taatcaactg cttcgcttta agcgaatatt cgtcatcgcc
     7081 ataaccatcc tgctggctca tgtttgttgt tgaaagggcc tctaatatcc ccggatggca
     7141 gccttcgcta taatcatctc tgaaactgta catttggtct ttttttatta ataaaagtaa
     7201 agaaaaaaaa cggcgctaat gtacccatac gaataaaaac tacaattttt gaccgtttat
     7261 tatactgcaa aagccaaaag ctttgcttaa ttttgaaaaa atcataattg acaaaaatgt
     7321 ttttcattaa ggttcaggtt ctcgatataa tagatattac catagtagcc tatctaatgt
     7381 ataggtttta catcctgatt cgcggaacgg tagccatcaa tatttttatt actatattct
     7441 ctatttacct gttctggctt gtagccaaag cccttgatat gcaattatta ggcactatta
     7501 ttggccagat tatcggagtt ggggttatag ccttaatcat cgtatttcac caggaaatac
     7561 gaaggtttat gttaataatt gggacgaggt atatgtcacg gaccttttca ttaaaaaacc
     7621 ttgtatttgc aagcaaagga caacagcaac gcattcagat taaagaaata gcaagggctt
     7681 gcgtgaattt atcgaaagcc aaaattggcg gattaattgt tattgaaaga aactcagccc
     7741 ttcactttta tgccgacaca ggagtaatcc taaatgcaaa caccagtgca tcgcttatag
     7801 aatctatatt ttttaaaaac aaccctttac atgacggggc tattattatc attggcgaaa
     7861 aaatatttgc tgccagatgt atgctcccta tgtctcaaaa tgtacaactt cccgaacacc
     7921 ttggaatgag acatcgagca ggtgttagca tgagcgaaca cactgatgcc cttataataa
     7981 ttatttcaga agaaacaggg aacgtttcta ttgcagaatt tggaaaactt actcagaata
     8041 tcactttaaa gtcgttattg aaaacccttg aaaaagaatt tcatggagaa atcaaacaac
     8101 aaaatagggt tgaaccaata aagacttatt aaaaaccaag tgtactctta ggttcacgca
     8161 gagtcttgcg agaatgtacc aacactccca cacattgctt ctcatcttta tctttagcat
     8221 tggcgacttc aggcacagat acctcaacaa taatacgtcg agcttcggta ttggtaaagc
     8281 tgacttctgt tttgtaatta gcttcagcgt tgtcaaaaag aacggtttta tcttcgttgt
     8341 cttctatcaa cctgaacctt atatttttaa tcttaaactg ataacaaata cttatatgat
     8401 aatcttcatt actaaaagtc accagtttaa attcagaagt cattccataa gtaaataaag
     8461 cactcctgga ttgtccactc aggtaatatg ttgtattagg aaatttacat tgatattcat
     8521 ggtaaaaata acaatcctgt gcaatagcat aaaaactgag aaacaacatt actattgttg
     8581 ccattgcagt tttaaacggg ttcaatatgg tattttgtgt attcattggg tcaagattta
     8641 cattccttca attattcaat aaaacttttt cttacagcaa aaatcatttc ttttagctga
     8701 ctatactgtt cagcagtaat ctcaagttta ctgccacctt caaatatcaa gttaccttct
     8761 ttgtcttgtc taaccttagt tttggttttc ttacttgatt ttattgaaga aaaaagttca
     8821 tggactcctt taagttgttc agctacctgg gccacctgtt tatcatcact ataaatggta
     8881 agataagagt ataggttctc aaatgcatat ttaaaatcag caactcttgt tttcaatgga
     8941 ttatgagctt catttttatc tgttaattta aaggtgagat aaaaacattc aatataagcc
     9001 ccggcagcaa ttaaggccaa agttttctca cgattgttca ttataagaaa ttctactata
     9061 ccagcatacg actctgtaga tatttgcttc aaagagtcaa gattaagcag gtttttttca
     9121 atccttttgg ctatagaaag atcataagct gaagtaattt tcaccttctc gctaagcgaa
     9181 tgaatggctt tataatattc taaagaataa ttatacgatt caaaaagtgt aatataagac
     9241 aaatctgcaa gatacacacc aagcgcaatt gtttgggctt ttgaattgac atatcttgaa
     9301 atattgctta ccggattaag tatttcaggg ttatacttaa tattttcctt ttttataaag
     9361 gtaaataact catcaggagt aggaaaacgg taatatacac tttgtgtctt tttatctgtc
     9421 tggttaacag gcaaactgtc ggtagaaatt gcttgctcgc tgctgtttga agcatttcta
     9481 cagcccattg ccaacgttaa tgcaagaatg ccaaatgtta ttttcagttt catgaatgtt
     9541 ctcaattatt tgtgaataaa aatagaaaaa agtattgaat aaagcatagt ttgaaaaaaa
     9601 aatatcatgt agttaaattt cacctttcta tacttacatt gttaatgtga cattttacaa
     9661 taataccgct acgacttaac cattgtcgaa aataaattgt aaattacaga ccgaaaaaaa
     9721 acaaactaaa tcatttacaa cacactgatt aaaaacattt tacataattt atttttattg
     9781 tgaacaaaac aaaaacaacc tatcaggcca accctcgtca ctatgttgct gttgacagta
     9841 tcgtgtttgg ttttgatggt gaagatttaa aaattttatt gataaaacgt aaaatagaac
     9901 ctctcaaagg tgaatggtcc cttatggggg gctttgtcca agaaaaacaa agtattgacc
     9961 aggcagccga aatagtattg aaaaacctta ccggcctgga caatgtatat cttgaacaac
    10021 tctctacatt tggtgaagta caacgcgacc ctattgacag ggttatatct gttgcatact
    10081 atgcgcttat caaacttaat gagtacgaca gacaaatagt acacaacaat ggaggcgagt
    10141 ggtttagtat atctaattat cccaagctta tccttgacca tgaagaaatg gtacaaacag
    10201 ctattagtaa acttcgcaga aaatgccgca ctcaacctat cggatttgag cttttaccag
    10261 agaagtttac aataccacag ttacaaagcc tttacgaagc aatatggatg caaaaattag
    10321 acaaacgcaa ttttagaaaa aaatttcttt cgatgaattt attggataaa ttagaagaaa
    10381 aagataaaga aaattctaaa aaaggggctt tttattataa atttaataaa gaaaaatacg
    10441 aacaattaac aagccgcgga tttattttcg aaatttaaac aaataaccta cttattaata
    10501 atgtacaaac taacgaatta tggaaatgaa ttttgattta gcgtggatag actatgctgt
    10561 gatggcggtc tatttcattt ttgtgctggg catcgggtgg gcgctaaagc gctttatgaa
    10621 agatgctaat gcgtttttgg aagctggcag gtcaatgccg gcatgggttg caggcctggc
    10681 atttatttcg accaatttgg gggcgcttga ggtaatgggt atgaccgggt cgggtgcaaa
    10741 atatggcctt ttgacggccc atttttattg ggttggtgct atacctgcca tggtgttcct
    10801 tgccatattt atgatgccat tctattatgg ttcaaaagcc cgctcagttc ctgaatacct
    10861 caaactcagg tttgatgaaa aaaccagggg catgaatgct tttatgtttg cagccatgac
    10921 catcctggcc tctggtattt caatgtatgc attggcaata ttaactgaaa aggttcttgg
    10981 atggaacttt catgttagct tgattgtttc ggctgcgata gttctgggat atacatatct
    11041 tggaggctta tcatctgcta tttacaacga agtcttgcag tttttcatca tcgtaatagg
    11101 tttattgcct attgtatttc ttagtttgaa gcaacttggc ggctggtcag ggttagagca
    11161 agcccttgct cctgttgcca tacaaaacaa ctttgctgct gatgcctaca ctacaacatg
    11221 gaaatacact ggttcgggag gctcaaaccc tctgggtgta ccttggtatg gtatatttat
    11281 gggactaggc tttgtattat cttttggata ttggtgtaca aattttctta ttgtacaacg
    11341 tgctatgtct gctgaatcta caactgccgc acgcaatact cctttgatag gagcacttcc
    11401 taaaatgttt attccttttc ttattatagt tcctggcatt gcagcccttg cccttatgaa
    11461 tttgcccggt agtggtttta gtttgccggt aaatgcagcc ggacagccaa tttatgatta
    11521 taccatcatt gccctattga aacaatattt acctgccggt gttcttgggt taggtatcac
    11581 cgctcttttg gcctctttta tgtctggtat ggctggcaat gtttcggcat tcaataccgt
    11641 atggacttat gatatttatc aaagctatat cagacctgca acaggaaata aagaagccga
    11701 tgataaacat tatttatttg ttggccgtat ggctactgtt tttggggttt tagttagtgt
    11761 tgcagctgca tacctggcca gtatgtttgg aaacatcatg gactttttgc agaccatatt
    11821 ttctatgatt aatgccccgc tgtttgctgt aattttctta gggatgtttt ggaaacgcag
    11881 tacgggccat gctgcttttg ccggtttgat tgcaggtttg accattgcct ttttgcacca
    11941 cggattaacc ttccctgccg gagctgacac cctggtaaaa ggcggatgga tagccaaact
    12001 tcacgaatac ccggttgaaa tggcccaaaa tttttggact gccatttatg cttgttttac
    12061 aagtgcctta gttacggttg ttttatcatt aatgaccaaa caacaaaaga ctgatgaaga
    12121 cctcaaaggc ctggtttact cgttgacccc aaaattgcag gagtcacatg ttgtatggta
    12181 caaacgctct tcatcacttg caatacttgt gattgcaata gctttgcttt taacaatatt
    12241 attctggtaa aaatataaaa tagataaaaa tgggattaga tataaaatat ccaatagggt
    12301 tgatgtttac aatccttgga atcatgataa caatttatgg ttttgccact attaatgaca
    12361 ctactttata tcaaaagtca ttaggcatta atattaactt gtggtctggc atctcaatgc
    12421 tggtttttgg cattgttatg cttgttttat ccaaaaaaaa taaaaaagaa gaatagcgct
    12481 tagcttagct tcaaaaatgt taaaaggtgc tggaaatccc ggcacctttt ttgtttgata
    12541 taccatcact tcatttgtaa taagagataa aattccaata accaataaaa ccaaattaac
    12601 cattaggaag cttttaaagc caaactatat gatttagttg ggttaaccct ctcgttatat
    12661 acgagacaag ctgatttaga aattgtttga aagattttat taaattattg aattgccatt
    12721 tcttcgatta agcattttta atgcttaatt tggggaaaaa acaaaagcag ccggaagaac
    12781 tccggctgct tttaacctaa ccctaaacct taattaacct aaaaacacta cttaacctta
    12841 aaccttaaat cattatgatt aataacctgg tacaaagata ctatcaaaag caagtctttt
    12901 accagaaaag caagatgtgt ttcgtcattc taaaaaggtg atttaggtta ccgtgttatc
    12961 ggcaaatact atctatgatt tttttataca cctctgcaac agtaagcccc attgttttaa
    13021 tctgtttagg gataatacta ttagggctca tccctggcac agtatttacc tcaagaaaat
    13081 aaggggcccc atcgttgata atgtagtcaa tccttacaat tcctttgcag ttaagagctt
    13141 tgtatatttt aagcgatgtt tgctgtactt ttcgggttat ctcatcgctg attcgggctg
    13201 gtgttatttc ttcggacaat gcagcattgt attttgcttc aaaatcaaaa aacgaagtct
    13261 tagaaactat ttcagttaca ggaaaaacta actcttcttt atctataaca taaaccccat
    13321 tagttacttc tgtaccgctg ataaattgct cgataagtac ttcgttgctt tcggtcaacg
    13381 catgttcaat agcccccagg agctgatttt cttcatctac cttggttact ccaaaacttg
    13441 accctgaagc attaggtttt acaatacatg gaaaacctac aacacttgtt atatgaggaa
    13501 catcccattt ttcgttttta ctaatgagaa ttcctttagc agtattaatg ccaaaattat
    13561 ttaagaaaag cttgcaggca tatttgtcaa aagtcagggc agaggccaaa actccgcaag
    13621 aagtatatgg cacctgcaaa caatcgaaat agccttgcaa catcccatct tcgcccggtg
    13681 tcccgtgaat agcaataagg gcacaatcaa atttgacctt ttgggcatca atgcttatgc
    13741 tgaaatcatt tttatccaca tctaccaacc gaccatctgg aagcttagct ttccattcag
    13801 caccttttaa ttttaccaag aacatgttat acccggcttt gctaacctct tcagccaaca
    13861 tagccccgct ttgcaccgaa ataacgtact ctgaagagtt acccccggca actaccgcaa
    13921 tatttttagc catctgtaat acaattgaaa taaacaacct gcttgacgga gaaagcagaa
    13981 atcagaactt cacttccggg gctttatcag cacctgctgt tgcctcaaag tatggttttg
    14041 aagaaaaacc aaacatttta gcaaataaaa gttgtggaaa tgaacgaata taagcattat
    14101 aatcttttgt caattcatta aacttcattc gctcaatagt gatacggttt tcagtacctt
    14161 caagctgtga ttgcaattca agaaaattct gattagcctt caattctggg tatttttcca
    14221 ctaccaccat caaacgcgat aaagcacttt ggagcccatc ctgagcttgc tgaaattgct
    14281 tgaaagactc ggcatttaac ttagcaggat caatatttac tgaggtagct ttagctctgg
    14341 cttcaataac agctgttaat gtagattttt caaaatctgc atatcccttt actgtattaa
    14401 caagattagg aataaggtct gcacgacgtt gataaacgtt ttgcacctgt gcccactgcg
    14461 aagatacggc ttcttctttt tgaaccatac tattatagcc gcaactgctc aacattggaa
    14521 ctaccaatgc caataacaac agacgagtta tgtttttcat atttttaaga attttgtttc
    14581 tttacaaagt taattttttt atcgtgaata aaatgcaaag aattcttaaa acctcagaag
    14641 atggctcaag tactgtttat agcgaacaga ctggtgagac atatcattct attcatgggg
    14701 cgtacaccga atcaatgcat gtatttatca ataacggtct atctttagta aaaagtaata
    14761 aaattaatgt attggaaatt ggctttggaa ctggacttaa cgcatggtta agctatatat
    14821 atgctataaa taaagggctt gaaatatctt atgatgctta tgagctatat cctctccctt
    14881 tttccttagt aaatctgctt acttataaag atatttatcc tgagaagcaa gaaaaatttg
    14941 aattgataca tagtgtgcct tgggaaacca ctgctaaaat tgaccataat ttttatttgt
    15001 caaagaaatg ctcagatatt actgtgtgta cattcaatgg catttatgat atggtttatt
    15061 ttgatgcctt ttctccggac agccagccgg agatgtggac tggtaaggta tttgaaaaaa
    15121 tatttcaaga tatgaaccac gaaggaatat tgaccaccta ttgtgtaaaa ggtattgtta
    15181 aaagggcttt aaaagaagtg ggctttaaaa tagaagtatt accagggcct ccagggaaaa
    15241 ggcacatgct gagggcatgg aagatgtagg gtaaataaga aagacgcttt gcattttcgg
    15301 aaatgcaaag cgtctaacat gtcaggctat atagtaagaa ttacctttac aatcaatgtg
    15361 ttgtgaacag aatacagtaa ttctcaatca aaattcttca tgaaagaccc agagggtcaa
    15421 tagtatggta gataaatatt taaacagggt ttacaagagt atcgcaggta cgaaagagaa
    15481 actttaaaag gtcatgaagt caaaaatatc gtgtttttca aaaatcactt tcgtcccatt
    15541 cgggactaat attacagttg cactaattac taccatacct gagtccctcc gggacatttg
    15601 gttgactttg caaaatgcaa aatattttta aatcaaagga aatgattctg aatttttaag
    15661 attagagagc aacgtagttg cggtatgttt gtagaaagtt ttttcacttc cgaagtctta
    15721 aagacttcgg aagtgtatgt tatagcgtaa tcaatatcaa cgtttgggcc aaaagtatcc
    15781 gcagaaacat ggtcagtgga tatactgttg cataagcagt agattgggca tgaacaggag
    15841 caaggctgtt ggcaaactca agagctggag gatctgtcat agcaccggac acgataccgc
    15901 atattttcag gtaattcatt ttcatcaggc gtgcaataac agaggctaag aatattggaa
    15961 taaatgttat ggctgctcca tataaaagcc agatataccc accattaaca agggttttta
    16021 caaattcact acctgaagat aaacctacac atgcaagaaa taatataatg cccagttcgc
    16081 gcagcatcat attggccccc ggggtcatat aaaaatcaag gttcttgatt cgtcctttat
    16141 agcccaatat tatggcaatt aaaagtgggc cacctgccat acctaatttt gccggagctg
    16201 gcaagcccgg gataaataag ggtacgcttc ccaggataat accaagaaat atcccaagaa
    16261 aaataggtat tgtattggga tgtgcaagtt ctttaacaga gtttccaagt tcttcgcgta
    16321 tctctggaag caaatcacgc ttgcctacga cccgcacagt atcgccaagt tcgacagtag
    16381 tatcatgcgt aggtaaaatc tccatccctg ccctgaatat gcgggtaata ttggcttcgt
    16441 aacgacgata aataccaatc tgttcaatag ttttgcctgt aagtttgcga ttggttacta
    16501 atacatgaaa catggccaaa tcccctgaaa tttcacgttt gctgccgatt ttcacttcac
    16561 ccatcttcat ccgcaggttt tcaatgaggt taatagcaga tactccgtaa atgacatctc
    16621 cctgctgtat cttttcgttt tcttttgcta caagaaactt tccatctctc tcgatacgag
    16681 aaataacaag ctctttgtcc acaatctttt tgatatattt tatttctttc ccaaacatat
    16741 tgggattaga aatgtaaatc tcgacgctct caagtttctt ctgactattg ctatattgtc
    16801 cattataatc ttctacttcc ttgtttacct tgatgctaaa aataaaccga accaatatca
    16861 tggtgataat gataccaagc accccaaaag gataagctac ggcgtagccc atacctgtta
    16921 cagcagcggc atcagcttgt ccgacttcat ttaaagcttg ttgggcagca ccaagtccgg
    16981 gggtattggt aacagccccg ctcataatac cagtaatgac agctggtgaa actcccaaaa
    17041 ctgcatgaat aaaataggct attgaaaaac cggtaacaac aatgcctgct gcaaaaaggt
    17101 ttagtttaag cccatcatta cgaaaagtgc taaaaaaacg agggccaacg tcaataccaa
    17161 tagcataaac aaacaagatc aggccaaact ccctgacaaa atgtaaaaca tgaggatcgg
    17221 cctgggcccc aaagtgggca aaaattaacc ctgagaacag caccccggct atccccaact
    17281 ttactttttt aaactccaat ttgcctatta aaattccgac aaatgcagca aaactaatga
    17341 aaagtatagt tgtagcagta ccggtcccgc taaataagct ttgaataaaa ttcatatatt
    17401 atatttttat actttcaaaa gtaaactcaa ctgtcataaa tatataaatg ttcggcatag
    17461 catcctatat ttgagttaaa aaaatcataa agcgttgaat tgcttcatgt cttttataac
    17521 aaataagtag gtgtttagtt tcaccacgtt gttaatggaa aaacgtctcc cccttccttt
    17581 gcgaa
//
LOCUS       DAOSPF010000064        17143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3357_length_17143_cov_8.386704, whole genome shotgun sequence.
ACCESSION   DAOSPF010000064 DAOSPF010000000
VERSION     DAOSPF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_17143_cov_8.386704"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            556..1374
                     /locus_tag="PK252_11545"
     CDS             556..1374
                     /locus_tag="PK252_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15384.1"
                     /translation="MHEIKKYPVQHPLLKNYIKFFWELRVSHLELHHKLIPQRNINLR
                     FNLSDTPHILSVDGDEHVLETVYFSGLQDHFSNAYLKLSGEVHTLGICFFSHGFYPFV
                     KMPVAEFKNQVLGTAEVGCKFPDLLSPRLKEASDIASRLSILEDELVQIIVHSHEYDE
                     HFQKIFIKLIDCKNSLQISEFSSQNGFSLRNLERMFNKYVGMPASTFSKLNRFHTCMN
                     QLLMKDFSKLSDLAYDYGYFDQMHFIRDFKRFAGDTPKNFVHQNSSILQIGKLA"
     gene            1449..1757
                     /locus_tag="PK252_11550"
     CDS             1449..1757
                     /locus_tag="PK252_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15385.1"
                     /translation="MKTSIITEFVKIEVLQTTTLEQLMEKADIVNAFLQKQDGFIDAE
                     LVKAVDDNVWYFIYHIESMEKLKAVGEKLRSSKMFDHITPLIVPESLSVNFFEQTKKW
                     "
     gene            1791..2591
                     /locus_tag="PK252_11555"
     CDS             1791..2591
                     /locus_tag="PK252_11555"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOT15386.1"
                     /translation="MELQKLKLVFFSPTGTTKTVVQSIAKGINSNLSELINITTPEAR
                     RNSLFTTEDELLVVAVPVYMGRVPAVVSGWLQTIKAKDTLTVCVVVYGNRAYENALLE
                     LKDILVQCGCRPIAGAAFIGEHSFSSPELPSSVGRPDAKDIQQAELFGKRIKELLQAV
                     ASSKQIECANIPGSYPYGGVTDLWHVDFIAVSDQCSQCGVCARGCPTGAIDSLQSSVI
                     DKEKCTLCCACIKHCPRNARTMKAGLMKDAAIRCTSFKERKEPECFLP"
     gene            2745..3995
                     /locus_tag="PK252_11560"
     CDS             2745..3995
                     /locus_tag="PK252_11560"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT15387.1"
                     /translation="MLKKADIISIGDEILIGQIVNTNAAWISTRLNELGIGINRVTSV
                     GDSHEEILAILDEASKRSDIIIITGGLGPTSDDITKPALCSYFNTRLVHNEIVYAHLE
                     TFIKQRGSIMNEGNKSQAMLPECCEVLQNNWGTAPGMWFEKQGIIYVSLPGVPFEMEG
                     LMNEYVLPKLQAKFGIEHIIHRTILTHGIAEAQLATILTDWEKTKNENVKLAYLPSPG
                     MVKLRLSVYGEKNGSALIQQEEKKLHEIIGNAIYGYDEDTFESVIGKLLKERNSTLSL
                     AESCTGGTIASKIVSISGSSDYFKGGIVAYSNEIKMKELGVPAETLVKHGAVSQQTAE
                     AMAEGVRTRFETDYSLSVTGIAGPLGGSEEKPVGTVWVAVSSKEKTIAKKFRFGDNRQ
                     RNITRTAMAAMNMLRLFIEGKEIF"
     gene            4069..4701
                     /locus_tag="PK252_11565"
     CDS             4069..4701
                     /locus_tag="PK252_11565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT15388.1"
                     /translation="MSVLVGKKAPKFSAKAVVNGNEIVENFSLDQYEGKKYVVFFFYP
                     ADFTFVCPTEIVAFQDHLAEFEKRNVVVVGCSVDSEFSHWKWLQTELKDGGIKGVKFP
                     LVSDLSKTISENYDVLAGRYEIGDEGEAYFEGTPMAYRGLFLIDKHGIVRHQVVNDMP
                     LGRSVEETLRMVDALQYFEKNGEVCPADWKPGKKAIKATQESIADYLGKH"
     gene            complement(4798..5319)
                     /locus_tag="PK252_11570"
     CDS             complement(4798..5319)
                     /locus_tag="PK252_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT15389.1"
                     /translation="MSTGKKLIAHSAENNAQHEFTFKNDYDFYYKNQKLKFDIIEEAD
                     GFTLVSIQGVKYPVEIVSQKQNTYEILVNGVSYTFSIETPFSLKRKLMLANNADKSGK
                     VKVKAPMPGKILDILVMTGQKVNKGDTLLILEAMKMQNTIMAQNAGIVQSISVKANDI
                     VAKDDVLVEINQE"
     gene            complement(5363..6847)
                     /locus_tag="PK252_11575"
     CDS             complement(5363..6847)
                     /locus_tag="PK252_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOT15390.1"
                     /translation="MIKSILIVNRGEIACRIARTARRMGIVTFGIRTRKEPNAVYLSA
                     VDKILDFPDTDGKVAEFLDIENLIALAKNNKIEAVHPGYGYLAENAHFAKRCREEGIK
                     YIGPAPEVIQAMGDKIMAKKIAAKCKVPMLGGSLDSVKDVAEAAKLAQKVGFPVIIKA
                     AAGGGGRGMRIVRTEAELPVMFKLATSEAQSAFGDPSVFIEKYLENPKHIEFQVVADS
                     HGNVVHLGERECSVQRKHQKLLEEAPSPALDEKLRAKMAKAAVALAKEVQYESLGTVE
                     FLLDKFGNFFFMEMNTRIQVEHPVTEAITGLDLVELQIRIASGEKLPVTQKEVSLNGW
                     AIECRINAEDVQSGFAPSMGTIRRMRLPQGNNIRIDTGIMEGSEITPYFDSMVAKLIV
                     HGKTRKEAIESTLQALRQFHVKGIKTTIPFCKAVMLNKVYRNGSFDTSFIETEMDSMV
                     HREEKEEFIAALLATYFYTHETTPVVSSESSIDPWVLKRRIKHL"
     gene            complement(6857..8398)
                     /locus_tag="PK252_11580"
     CDS             complement(6857..8398)
                     /locus_tag="PK252_11580"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT15391.1"
                     /translation="MGKAYDKFIERQQLLEKQYDDAYYEKQHNRGKLTARERIALLFD
                     EGTFEELDAFAPPASVSFGKSTKAFGDGVITGYGKVNGRQVYAFAQDFSVLGGSLGAV
                     HAAKIIKIQDMALKTGCPIIGLIDSGGARIQEGVQSLNGYAGIFRRNVLSSGVIPQIS
                     VIMGPAAGGAVYSPALTDFIFMSAGTSYMFVTGPDVVKQVLNESVSPEELGGASIHST
                     RSGVADFAFNDDENTILGVKMLLSFLPSNNIENPPFVASDDPITRIEPSLRDIVPDDP
                     DKPYDVKEVIKLVVDHGRFFEVGENFAPNFVTGFARMGGQVVGILANQPRIMAGTLDI
                     SSSTKGARFINICDSFNIPLITFEDVPGFLPGTDQEHNAIIKHGAKLLYAYTYATVPK
                     ITVVLRKSYGGAYIVMNSKGIGGDFSFAWPTAEIAVMGPEGAIAVLYRKELAEAADPV
                     ALKKQLAQKYREDIANPYIAEEKGFIDEVIDPATTRLKVIRALQSLEKKSISIPKRKH
                     GNIPL"
     gene            complement(8577..8720)
                     /locus_tag="PK252_11585"
     CDS             complement(8577..8720)
                     /locus_tag="PK252_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15392.1"
                     /translation="MNKQKALMVYSYPSIVLALDETAMIVAKQKYQNKMWVFHLHTSN
                     GQY"
     gene            complement(9012..9728)
                     /locus_tag="PK252_11590"
     CDS             complement(9012..9728)
                     /locus_tag="PK252_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT15393.1"
                     /translation="MAGFGKWIGGGLGWAFGGPIGALLGFAIGTVFDNIEVSTNTRVN
                     TTRGDFLVSLLVLMAAVMKADGKVMKSELNFVKEYLQRSFGPEDTAQALKILQGLLQK
                     PIPVHEVCLQIRQHTDYPSRLQLMHLLFGIALADGAISQEELHTIEDICHQLHISPAD
                     LASIKAMFIEDTEQAYKVLEIEKNASDEDVKKAYRKMALKYHPDKVAYLGEDVKNAAN
                     EKFKKVNEAYEKIKKERGFK"
     gene            complement(9733..10323)
                     /gene="coaE"
                     /locus_tag="PK252_11595"
     CDS             complement(9733..10323)
                     /gene="coaE"
                     /locus_tag="PK252_11595"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT15394.1"
                     /translation="MIKVGITGGIGSGKTTISKIFETLNIPLFNADIEAKKLFETEEV
                     KKFYLAHFGDAVFSENKLDRQKISNILFSAPDKLMLVNSFIHPLVVELFDNWTIKHIH
                     ADYVLKESAILFETGLYKDLDYNILVTAPVETRIKRIIERDKLPQEQIELRMANQWTD
                     EAKVPLADFVIENEDNSLVIKKVLDIHQKILASIKK"
     gene            complement(10316..11290)
                     /locus_tag="PK252_11600"
     CDS             complement(10316..11290)
                     /locus_tag="PK252_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15395.1"
                     /translation="MFGQNKKGFKTRQKVLTFLFFFLLASLFWLLNKLNNTYSADVTF
                     PIKYVQYNPDKEMVRPIPSEITLNVTGQGYVLLRNMLFAKHYPVTFRVVSLNLLEIPS
                     KNNQFLLFTKTLKENIQRQLGSDLQLNYIIPDTLLYTFSPVVHKKVKVVPDIEVKTAT
                     QYMQEGRFIVIPDSVVISGPQVIIDTINFIKSIPSKLLNAKKSTSLEVDLITPEKTSL
                     SSKEVQVTIPIEKYTEINLNVPLKALNVPQNKRLITFPPSVSVICRVSLSNYKKVSVS
                     TVNAHVDFNDATVKSASRLKVNITDYPSFVKIVSYTPKNVEFLIEKND"
     gene            complement(11422..11730)
                     /gene="yajC"
                     /locus_tag="PK252_11605"
     CDS             complement(11422..11730)
                     /gene="yajC"
                     /locus_tag="PK252_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT15396.1"
                     /translation="MNMLTVLLQAPAGNPLVQLLPILLIIVVFYFFMIRPQMKRQKEL
                     KNFRDSLQKGDKVITTGGIYGRIVEIQDHVVFVEIDQNVKIKVDKAALLKDPSDIEQK
                     "
     gene            complement(11784..12728)
                     /gene="nusB"
                     /locus_tag="PK252_11610"
     CDS             complement(11784..12728)
                     /gene="nusB"
                     /locus_tag="PK252_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT15397.1"
                     /translation="MISRRLIRIKVLQTLYSAQSREERSLSILEKEYFHSINKTYELY
                     LLFLQVPIELAKAEEQKIDIGLQKFRPTPEERNPNMRFCQNAVIERLRKNVELTNVIN
                     EKKVNWANNADFFKTLNIQLRQYDFFNDYMNKPSVTFEDDKKILLNIIDKIVPNTEAF
                     DSILEDMSIFWNDEVEFVLNMVSKTIKSFEEGKLDKPLMKVFKNEDDQEFAKQLIRNT
                     LNNQDEYNKLIRKYSQNWDMSRMAVIDHLIMQTAIAEIVEFPSIPVKVSLNEYIDLAK
                     LYSTDNSNTFINGILDSVVKDLKSQGKVVKTGRGLIDN"
     gene            12961..14910
                     /locus_tag="PK252_11615"
     CDS             12961..14910
                     /locus_tag="PK252_11615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT15398.1"
                     /translation="MKKLICLLSATAVFLASAISQEETILTIDKQKISKAEFVRTYMK
                     NNNNASFDEKSLKEYMDLFINFKLKVKEAERLGYDTMSSFIKEYNSYRSQLVKPYLTD
                     KETDEKILKEAYERKCEDIHARQIYFAASELVKPKDTLAIYQRAMEAYNKLISGVPWD
                     SVHSQYSQERNKQKKGDIGYFNSLQISYPLENICFRLKKGEISKPQRTIYGYHIVEIL
                     DRRPSKGEIRAAHIMVAFPENSTPQSVDSAKAKIAEIYQKLKQGESFEELAKQYSDDK
                     RSAAKGGDLGWFGSGDMIPEFEKNAFALEKDSDYSAPFRTSFGWHIVKRISTRPVQPF
                     EALKTSLSQKIAKNERNEEGRKALIRKIKAEIKFKDNLNTALLLVPYLDSSAFRGKWN
                     AEKAKPIFDKELFTLGNKKYFMQDFAQYLSSYRFKLPASYEMSITYFYNKYIDDAIIA
                     FEDSRLESKEPELKYLLQEYHDGIMLFNLMDQKIWSQAAKDTVILAKYFEDHKTQYVW
                     GPRVEALVVSSPDKELVNKAYAFANDFANGKISAEKIWQSICPDTTQPCLNCQVNLFE
                     KGDNQILDSLGWDKGVTPIVNREGKFGFFVKTQHFEGRPKTYEESKGTVIADYQQFLE
                     DQFVKELKKKYAVVVNQKVLKSLVK"
     gene            14948..15790
                     /locus_tag="PK252_11620"
     CDS             14948..15790
                     /locus_tag="PK252_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15399.1"
                     /translation="MLKYFLIIFVFAMPAWCSKKDKPEDKPLAKVGDTYLYKSELNGY
                     FRSGTSPEDSIIMFKALVEKWIRKQLIVEKAKLNLTREEMDVEKELEDYKTSLIIYKY
                     EQKMLKEKLDTNVSNAEIEKYYNQNIQSFNLSSPIVKAQYIKLSIKSPQLDKLKLWLK
                     TDNPENVRLIDEYCFKYNVKYNYFNDEWISFENIKMMMPFTSNVTENYLSMNKLIELT
                     DSTNLYLLKIREIKFSGTTAPLEFVKPNIKSIIILKRKQQLINELEDKIYFEALDKNK
                     FELL"
     gene            15808..17142
                     /locus_tag="PK252_11625"
     CDS             15808..17142
                     /locus_tag="PK252_11625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT15400.1"
                     /translation="MLKKSLFVGLLFSFSLLYSQDEKLADKIVAVIGKSIVLQSDIEQ
                     QMLQYKAQRQNIERCEVFENFLMQKMLVNQARIDSIEISEVNVEMSLNNRMDYFLQQA
                     GSQEKLEEYFNMPLNQIKDEMRESIREQMLTQKMQSEITKNIRITPSEVKEFYKSLPP
                     DSIPMIDQQIEYKQILLYPSFAKQSIYETREKLLDIRKRILAGEKFSTLAVLYSEDPG
                     SVTNGGEIGFMSRSELDPAYATAAFNLKPGAVSGIVESQFGFHIIQLIAREGDRVNTR
                     HILIKPKITAKELQLAFNRLDSIARAIKADSITFEKAALIYSEDKTTRFASGQVVNPY
                     TNSTLFELDQLPKEDFYILSKLNVGDISQPFQSKDENRKDAYKIVKLTGKKDPHRANL
                     DDDYLLIQDLALESKKKKYVDEWIENKLSTTYFRIDPTFDCNFKINAWRKKN"
BASE COUNT         5516 a         3251 c         3161 g         5215 t
ORIGIN      
        1 aagtagctca gttggtagag catcagcttc ccaagctgag ggtcgcgggt tcgaaccccg
       61 ttttccgctc aaaggcaagt atctcggaat gaaagcttgc ctttttttta tcaaacatta
      121 aagtttttat tagattcctt ttttgattgt tttgctgaag tagctctgtt ggtagtctcg
      181 cctcaggcga gatcccaagc tgagggtcgc gggttcgaac cccgttttcc gctcaaaggc
      241 aagtatctcg gaatgaaagc ttgccttttt tttatcaaac attaaagttt ttattagatt
      301 ccttttttga ttgttttgct gaagtagctc tgttggtagt ctcgcctcag gcgagatccc
      361 aagctgaggg tcgcgggttc gaaccccgtt ttccgctcaa aggcaagtac tgtcaaaacg
      421 tttagctttt tcaaaaagct aaacgttttt atttatattc attcaatttt gaaaaactgt
      481 cattcattgg ttttctttag accttttatt tgaagatggt gtaattttag cagcaataaa
      541 ttttcgttgc ttgtgttgca tgaaattaaa aaatacccgg ttcagcatcc tttgctcaag
      601 aattacatca agttcttttg ggagcttcgt gtaagccatc ttgagcttca tcacaaattg
      661 ataccccagc gaaatataaa cttgcgtttt aatctgagtg atacacctca cattttatcg
      721 gtagatggtg acgagcatgt tcttgaaacg gtttattttt cggggttgca agatcacttt
      781 tccaatgctt atttgaaact aagcggagaa gtgcatacgc tgggcatttg ttttttctcg
      841 catggctttt atccatttgt gaaaatgcct gtggctgaat ttaaaaacca ggtacttgga
      901 acggctgaag ttggttgtaa atttcctgat ttattaagcc cccggttaaa agaagcttcg
      961 gatatagctt caagattaag catcttggaa gatgaacttg tgcaaataat tgttcacagc
     1021 catgaatacg atgagcattt tcaaaaaatt tttataaaac ttattgattg taagaattca
     1081 ttgcagatat cagagttttc ttctcagaat ggctttagtt tgcggaattt agaacgtatg
     1141 ttcaacaaat acgtaggcat gccagcaagt acatttagca aattgaaccg ttttcataca
     1201 tgtatgaacc agcttctcat gaaagatttt tcaaaacttt cagacctggc ttatgattac
     1261 ggatactttg accaaatgca ttttatcagg gatttcaaac gttttgcagg cgatacccca
     1321 aaaaactttg tccatcagaa cagttccata ttacaaatag ggaaactggc atagttgtcg
     1381 tttttgtact atttctttgg ttttggggca agtaattttg tcttcataaa ttttaaaaac
     1441 aatgagttat gaagacaagt ataattacag agtttgtaaa aattgaagtt ttacaaacaa
     1501 caacgttgga acagctaatg gaaaaagctg atatcgtcaa tgcttttttg cagaagcaag
     1561 atggctttat agatgcagag ctggtgaaag cagtagatga taacgtttgg tattttattt
     1621 accatataga aagtatggaa aagctaaagg ctgtgggtga aaaattgcgc agtagcaaga
     1681 tgttcgacca tattacgcct ctgattgttc cggaaagcct tagtgtaaac ttttttgaac
     1741 aaactaaaaa atggtaacaa tctgtgattt aaaacattgg agggaagaat atggaactgc
     1801 aaaagttaaa actagttttc ttttcaccaa caggcacaac gaaaactgtg gtgcaaagca
     1861 ttgctaaagg cattaattct aatttgtcag aactgataaa cattacaaca cctgaagcgc
     1921 gtagaaatag cttatttact accgaggacg aattgctcgt tgttgcagtg cctgtttata
     1981 tggggcgtgt acctgcagtt gtttcaggat ggttgcaaac catcaaagct aaggatactc
     2041 ttacagtttg tgtagttgtt tatggtaatc gtgcatacga aaatgcgctg ctcgaactta
     2101 aagatatatt ggttcagtgt ggttgcagac ctattgccgg ggctgcattt attggtgaac
     2161 actctttttc aagtcctgaa cttccaagtt ctgttggacg tccagatgca aaagatattc
     2221 aacaagcaga attatttggg aaaagaataa aagagttgct gcaagctgtt gcttcttcaa
     2281 agcaaataga atgcgcaaat atcccgggca gttatcctta cggcggagtt accgaccttt
     2341 ggcatgttga tttcattgct gtgagcgatc agtgttcgca atgcggtgta tgtgccaggg
     2401 gatgcccgac aggggcaatc gattcgctgc aaagcagtgt tatagacaag gaaaaatgca
     2461 ccctctgttg tgcctgcata aaacattgcc ctcgcaatgc cagaacaatg aaggcgggcc
     2521 tgatgaaaga tgctgcaata aggtgcacct cctttaaaga acgcaaagag ccggaatgct
     2581 ttttaccatg atttctgctg ttatcaaaat caaagattgt ttttcattcg ttgaaagaat
     2641 tgataacttt ggccattata ttggaaatgc taagaatgtt ttgattttaa aaagtatgac
     2701 aagtaatttt taaaagtgga tcattctgga aaataaattt gaatatgttg aaaaaagcag
     2761 atattatctc cattggcgat gagatactta ttggacagat tgtcaatact aatgctgcat
     2821 ggatatcaac ccggctcaat gagcttggca ttgggataaa ccgtgtgacg tctgtgggag
     2881 acagccacga agaaatattg gcaatactgg atgaagcctc taaaaggtca gatattatca
     2941 ttattaccgg aggccttggt cctacatctg atgatattac taagccggcg ttatgcagct
     3001 atttcaatac ccgcttggtt cataatgaaa tagtatatgc tcatctggaa acatttatta
     3061 aacaaagggg cagcattatg aatgaaggaa acaaaagcca ggcgatgctc cccgagtgtt
     3121 gcgaagtgct tcagaacaat tggggcacag ctcccggcat gtggttcgaa aaacaaggca
     3181 taatctatgt ttcgcttccg ggagttccgt ttgagatgga aggcctgatg aacgagtatg
     3241 tattgcctaa acttcaggca aagtttggta ttgagcatat tatccatcgg acaatcctta
     3301 cgcatggaat tgcagaagca caactggcga ctatccttac cgattgggag aaaaccaaga
     3361 atgaaaacgt gaagttagct tatttgcctt ctcccggaat ggtaaagcta cgtctttcag
     3421 tatatggcga gaaaaatgga agcgctttga ttcaacagga agaaaaaaag ctacatgaga
     3481 ttattggcaa cgccatttat ggatacgacg aagatacttt tgaatcggtc ataggtaagc
     3541 tcctgaaaga aaggaatagc acattatcgt tggccgaaag ttgtaccggt gggacgatag
     3601 catcaaagat agtttccatc tcgggcagtt ctgattattt caaaggtggg atagtagcct
     3661 actccaatga aataaagatg aaagagcttg gcgttcctgc tgaaacattg gttaaacatg
     3721 gtgccgttag ccagcagacg gcagaagcta tggctgaagg cgtgcgtacc aggtttgaaa
     3781 cagactattc attgtcagtc acaggaattg caggcccatt aggtggttca gaagaaaaac
     3841 cggttgggac tgtttgggtt gcagtatctt caaaagaaaa aacgatagca aagaaattta
     3901 ggtttggcga caatcgccag cgcaatatta ccagaacagc tatggctgcc atgaatatgt
     3961 tacggttgtt tattgaaggc aaagaaattt tttaaacgta ttttgaactt ttgtttcaaa
     4021 cacgtgttaa aagcataaac aagtatttat taacctaaat aaaagattat gtctgtatta
     4081 gtaggaaaaa aagcacctaa attcagcgct aaagctgtag tgaatggaaa cgaaattgtc
     4141 gaaaattttt cattggatca gtatgaagga aaaaagtacg tagtattttt cttttatccg
     4201 gccgatttta catttgtttg tccaacagaa atagtagcat ttcaagatca tttggctgaa
     4261 tttgaaaaaa gaaatgtagt agtagtgggt tgttctgttg actcagaatt ttcgcactgg
     4321 aaatggcttc aaaccgagtt gaaagacggt ggtatcaaag gcgttaagtt tcctttggtt
     4381 tcggaccttt caaaaactat ttctgaaaat tacgatgttc ttgcaggtcg ttatgaaatt
     4441 ggtgacgaag gcgaagctta ttttgaagga accccaatgg catacagagg tttgttcttg
     4501 attgacaagc atggcattgt tcgccaccag gtggtaaatg atatgcctct tggacgcagc
     4561 gtagaagaga cattgcgtat ggtggacgct cttcaatact ttgaaaagaa cggtgaagtg
     4621 tgccctgccg actggaaacc cggtaaaaag gctatcaagg ccactcagga gagtattgct
     4681 gattatctgg gcaaacacta ataataaaaa ctaccaaaga agactggtga tatcagatat
     4741 cggacttcat acaaaaaaag aggctgctca aatgggcagc ctttttttca attcatatta
     4801 ttcctggtta atctccacca gcacatcgtc tttcgcaaca atatcattag ctttaacact
     4861 aatactttgg acaataccgg cattttgggc cataatagtg ttttgcattt tcatagcctc
     4921 aagtatcagc agggtatcgc ctttattgac cttttgccct gtcataacca gaatatccaa
     4981 tattttacca ggcattgggg cttttacttt taccttacca gatttatcgg cattattggc
     5041 caacatcagt ttgcgtttca atgaaaatgg agtttctatt gaaaaagtat aacttactcc
     5101 attcaccaaa atttcgtaag tattttgctt ttgcgataca atctccaccg gatattttac
     5161 tccttgaata gaaacgaggg taaacccatc agcctcttca atgatatcaa attttaactt
     5221 ttgatttttg taataaaaat cataatcatt cttaaaggta aactcgtgtt gagcattatt
     5281 ttcagcacta tgggctatca atttctttcc tgtactcatg ttttagaatg ttttagttca
     5341 aacctgttga ataaccagat tcttataaat gtttaatacg ccttttcaat acccatgggt
     5401 caatggaaga ctcagagctt accactggag tagtctcatg ggtataaaaa taagtagcaa
     5461 gcaaagctgc aataaattct tccttttctt cacgatgtac cattgaatcc atctctgttt
     5521 caataaaaga cgtatcaaaa cttccatttc tatatacttt attgagcatt acagctttac
     5581 aaaatggaat agtagttttt atccctttca catgaaactg acgaagagcc tgtaatgtac
     5641 tttcaatagc ctctttacgt gttttaccat gaacaataag cttagccacc atagaatcaa
     5701 agtatggggt tatttctgaa ccctccataa tacctgtatc tatacggata ttgttaccct
     5761 ggggcagacg catcctgcga atggtgccca tacttggagc aaatccagat tgtacgtcct
     5821 cggcattgat acggcattcg atagcccaac cattgagaga aacttccttt tgggtaaccg
     5881 gaagcttttc gccagaagca atacgtattt gaagctcgac taagtcaagt cctgtaatgg
     5941 cttccgtaac aggatgttcc acctggatac gcgtattcat ttccataaag aagaagttgc
     6001 caaacttatc gagcaaaaac tcaacagtac caaggctttc atactgaact tccttagcaa
     6061 gggcaacggc agccttggcc atcttggcac ggagtttttc gtccaaagca ggagaaggag
     6121 cttcttccaa tagtttctgg tgtttacgtt gaacagaaca ttcgcgttcg cccaaatgaa
     6181 caacgtttcc atggctgtct gcaactacct gaaattctat atgtttagga ttttcaagat
     6241 atttctcaat aaataccgaa ggatcgccaa aagcactctg ggcttcagat gtagctagtt
     6301 tgaacatcac aggcagttca gcctcggtac ggacaatcct cattccgcgg ccaccacctc
     6361 cggcagcagc cttaataatc accggaaatc caaccttctg ggccagttta gcagcttcag
     6421 ctacatcttt cacactgtcg aggctccccc caagcattgg gactttgcat tttgcggcaa
     6481 tttttttagc cataatctta tctcccattg cctgaattac ctcaggtgca ggcccaatat
     6541 attttattcc ctcttcgcgg catcttttag caaaatgggc attctcggcc agataaccat
     6601 aaccaggatg tacagcttct attttattat tttttgccaa cgctataagg ttttcaatat
     6661 caagaaattc ggccacttta ccatctgtat cgggaaaatc taagatttta tccacggcag
     6721 agagatacac ggcattaggt tctttcctgg ttcttatccc aaatgttaca attcccatac
     6781 gacgtgcagt acgggcaatt cggcaggcta tttcgccccg gttgacaata agtatgcttt
     6841 ttatcataaa taattgttat aaaggaatat taccatgttt gcgtttagga atactgatag
     6901 atttcttttc gaggctttgc aatgccctga ttactttcaa acgtgttgta gccgggtcaa
     6961 ttacttcgtc aatgaaacct ttttcttcag caatgtaagg atttgcaata tcttcacggt
     7021 atttctgtgc caattgcttt ttcagggcaa cggggtcagc tgcttcagct aattctttac
     7081 gatacaacac agctattgcg ccttcagggc ccataacggc tatttcggca gtaggccatg
     7141 caaaactaaa gtctccgcct atccccttac tgttcattac aatgtatgcc ccgccataag
     7201 acttacgtag tactactgta atcttaggaa cagttgcata agtataagcg taaagcaact
     7261 tagcgccatg cttaatgatt gcattatgtt cctggtctgt acctggtaaa aatccgggca
     7321 catcttcaaa agttatcaga ggaatattaa aactatcgca aatgttgata aagcgtgcgc
     7381 cttttgtaga tgaactaata tcaagcgttc cggccatgat acgaggctgg ttggctaata
     7441 tcccaacaac ctgaccaccc atacgggcaa aacccgttac aaagtttggt gcaaagtttt
     7501 cccccacttc aaagaagcgc ccgtggtcaa ctaccaactt gattacttct tttacatcat
     7561 aaggtttatc cggatcatct ggcacaatgt ctcgcaatga aggttcaata cgtgttattg
     7621 ggtcatcaga agcaacaaaa ggtgggtttt caatattatt tgaaggtaaa aatgacaaca
     7681 gcattttcac gcctaagata gtattttcat cgtcattaaa agcaaagtca gcaactcctg
     7741 aacgggttga atgtatagaa gccccaccaa gttcttcagg tgaaaccgat tcatttaata
     7801 cttgttttac tacatcaggc ccggtaacaa acatataact tgtaccggca gacataaata
     7861 taaaatcggt taacgctgga gaataaacag cgcctccggc tgcaggcccc attattacag
     7921 aaatctgggg tatcacacca gaagacaaaa catttcggcg gaaaatgccg gcataaccgt
     7981 taaggctctg aacgccttcc tgaatacggg ctccacccga atctatcaaa ccaatgatag
     8041 ggcaacctgt tttaagcgcc atatcttgaa ttttgataat cttggcagca tgtactgccc
     8101 ccagtgaacc tccaagcacg ctaaagtctt gggcaaaagc atatacctgc cttccattta
     8161 ctttaccata gcctgtaatt actccatcac caaaagcctt tgtggatttg ccaaacgaaa
     8221 cgcttgccgg aggggcaaaa gcatcaagct cttcaaatgt accttcatca aaaagcaatg
     8281 caatacgttc gcgggcagtt agcttacctc ggttatgctg tttttcataa taggcatcat
     8341 cgtattgttt ttcaagcaat tgctgtcttt caataaattt atcgtacgct tttcccattt
     8401 ttatgcatta ggttaatact atgtttatta tttgaagaca tttttattga tataaactat
     8461 ctgaacaata gccttttaac gacaaaaaaa ttgtcgaagt aaaattttta ttgattgttg
     8521 atattatttc catccgtata aaattttaat ttattgaaat ctaattattt aagctatcaa
     8581 tattggccat ttgatgtatg caaatgaaac acccacattt tattttggta tttttgtttg
     8641 gctacaatca tggctgtttc atctaatgcc agaacaattg aaggatatga gtaaaccatc
     8701 aacgccttct gtttgttcat tcaataacta tatgattatt taaatgcagg gcaaaaagta
     8761 gctgggggga tgtatatata taatattttg tcatttttat cattttaaac ataaattact
     8821 attaccaaat catcaaaatt atccttacca aaagtataat ttataggatg taaccaaaat
     8881 tttgtttaac tatttatgaa tatttatgca aatatttata aatgttcagc acaaatataa
     8941 acgactttca taaaactcaa ttaatattta taaatgaact aatattattt tataagctta
     9001 ctgttgaaac attatttgaa acctctttct tttttaatct tttcgtaagc ttcatttact
     9061 tttttgaatt tttcgttggc tgcatttttt acatcttctc ccagatatgc cactttatcg
     9121 ggatgatatt tcaaggccat cttacgataa gcttttttca cgtcttcatc cgatgcattc
     9181 ttttcaattt ccagcacttt gtatgcctgt tctgtatctt caataaacat cgcttttatt
     9241 gaagccaaat ctgcaggact gatgtgcaac tgatggcata tatcttcaat tgtgtgcagt
     9301 tcttcctggc ttatggcacc atcagccaag gcaattccaa atagaaggtg catcaattgc
     9361 aaacgtgaag gataatcagt atgctgcctg atttgaaggc acacctcatg caccggaata
     9421 ggcttttgga gaagcccctg caatattttc aaggcctggg cagtatcttc ggggccaaaa
     9481 ctgcgttgca aatattcttt aacaaagttg agctcagatt tcatcacttt accgtcggcc
     9541 ttcatcactg cagccatcaa caccagcagg ctgaccaaaa aatctccacg ggtagtatta
     9601 accctggtat tggtggatac ctcaatatta tcaaacactg tacctatagc aaaacctaat
     9661 aatgcaccta ttggccctcc aaaagcccat ccaagtccgc cgccaatcca ttttccaaaa
     9721 cctgccattg ttttattttt ttattgatgc taaaattttc tgatgaatat ctaaaacttt
     9781 ttttatcact aagctattgt cttcattttc aatcacaaaa tctgccaatg gcactttggc
     9841 ctcatcggtc cattgatttg ccatacgcag ctctatttgc tcctgaggca gtttgtcgcg
     9901 ctcaataata cgcttaatgc gtgtttcaac aggagcagta acaaggatat tgtaatcaag
     9961 atctttgtac aatcctgttt cgaacaaaat agcagattct ttgagtacat agtctgcgtg
    10021 gatatgtttt attgtccaat tatcaaatag ttcaactact aatggatgta tgaaactatt
    10081 gaccagcatg agcttgtcgg gggccgaaaa aagaatgttg ctaatttttt gcctgtccag
    10141 cttattttct gaaaatacgg catctccaaa atgtgccaga taaaatttct ttacttcttc
    10201 agtttcaaac aatttttttg cttcgatatc agcattaaac agcgggatat ttaatgtttc
    10261 aaatattttt gaaatggtag tctttccact gccaatacca cctgtaatac caaccttaat
    10321 catttttttc aataagaaat tcaacgtttt tgggagtata acttactatt ttgacaaatg
    10381 aaggatagtc tgtaatattt actttcaggc ggctggcaga ctttacggta gcatcattaa
    10441 aatcaacatg agcattgact gtagataccg ataccttttt ataattgctc agtgataccc
    10501 tgcaaataac agaaacagaa ggcggaaatg taatcaaacg cttattttga gggacattca
    10561 atgctttcaa aggaacattg agattgattt cggtatattt ttcaatagga atagttactt
    10621 ggacctcctt actcgataag cttgtttttt caggagtaat taaatctact tccaacgaag
    10681 tcgatttttt tgcattcaat aattttgaag gtattgattt tatgaagtta atggtatcta
    10741 tgatgacctg aggccccgaa atcacaaccg agtcgggaat aacaataaac ctgccttctt
    10801 gcatatattg tgtagcggtt ttaacttcaa tatccgggac tacttttacc tttttgtgaa
    10861 ccaccggtga aaaagtgtac aacaaagtgt cgggaatgat ataatttaat tgtaaatcag
    10921 accccaattg acgctggatg ttttctttta acgttttggt aaaaagcaaa aactggttat
    10981 ttttagaggg aatctcaagc aaattgagcg aaactaccct gaatgtaaca ggataatgct
    11041 tggcaaacaa catattgcga agcaatacgt atccctggcc ggtaacattg agcgtaatct
    11101 cagaagggat aggtctgacc atttccttat cagggttgta ttgtacgtat tttatcggga
    11161 aggtaacatc tgccgaatag gtgttgttga gcttattcaa caaccaaaac aacgatgcca
    11221 ataaaaagaa aaacaaaaag gtaagcactt tttgcctggt tttgaaccct tttttgtttt
    11281 ggccgaacat agtaaaaatt cttttggctt gtattggggt ataaaagtaa agtttttaaa
    11341 aatgagaaaa aacaaataag cccggaaaaa caaaagccga cctttaattt ctaaagatcg
    11401 gctttaatac gtgtaacaaa attacttttg ttctatatca cttgggtctt tgagcaatgc
    11461 agctttgtcc actttaatct ttacgttttg gtctatttca acaaaaacaa catgatcctg
    11521 gatttcaact atacggccat agataccgcc tgttgtaatc actttgtcac ctttttgcaa
    11581 gctatcacgg aagttcttca gctctttttg tcttttcatc tgaggacgaa tcataaaaaa
    11641 gtagaataca acaatgataa gcaaaatagg taatagttga accaaaggat tgcctgccgg
    11701 cgcttgtaat aaaactgtca acatattcat tttaaattag ttaataaaaa tttataatct
    11761 tattttttgc aaatatacca aacttaatta tcaattaagc cccttccggt ttttaccacc
    11821 ttaccctgac ttttgaggtc tttaaccaca ctgtcaagaa ttccgttgat aaaagtatta
    11881 ctattatcag tactatatag cttagccaaa tcaatatatt cgtttaacga aactttaaca
    11941 gggatagatg ggaactcaac aatttctgct attgcagttt gcataataag gtgatcaatt
    12001 accgccatgc ggctcatatc ccagttttgc gaatattttc taataagctt gttatattca
    12061 tcctgattgt tcaatgtatt acgtatcaat tgcttggcaa attcctggtc atcctcattt
    12121 ttgaacactt tcatcaaagg tttgtccagc ttaccttctt caaaggactt gatggttttt
    12181 gaaaccatat ttaatacaaa ttccacctcg tcattccaaa aaatgctcat atcttcaagt
    12241 attgaatcaa aggcctcagt attagggaca attttatcaa taatattgag cagaattttt
    12301 ttgtcatctt caaaagttac gctcggtttg ttcatataat cattgaaaaa atcatactga
    12361 cgcagctgaa tgttcaacgt tttaaaaaaa tcagcattat ttgcccagtt gaccttcttt
    12421 tcattgatga catttgtaag ttcaacattt ttacgaagcc tttcaatgac agcattctgg
    12481 caaaaacgca tattagggtt tcgctcttct ggcgtaggcc taaacttttg caacccaatg
    12541 tcaatttttt gctcttcagc cttagctaat tcaatgggga cttgtaaaaa aagaagataa
    12601 agttcgtatg ttttattgat actatggaaa tactcttttt ccagaattga caggctcctt
    12661 tcttctctgc tttgcgcact gtacaatgtt tgtaaaactt taattctgat aagccttctg
    12721 ctaatcattg gcaatgaact attaaatgag ccgcaaaggt agtttatttt gttcgatagt
    12781 atcaaatata aaataattaa ataatcaata aaatactaaa catatctata tcaaaccctt
    12841 aactaagttt aacttttaaa agcaattgtt tggccatgga taaacataat tttttgtaat
    12901 ttggcgcaat ttttaaagct aaattttttt aatgttccac taaaaaaaag ggattcaaaa
    12961 atgaagaaac ttatttgtct tttaagtgct acagccgttt ttttagcatc agctatttct
    13021 caggaagaaa ccattcttac cattgataaa caaaagatat caaaggcaga atttgtccgc
    13081 acttacatga agaataacaa taatgcttcc tttgatgaaa aatcattaaa agaatacatg
    13141 gaccttttta tcaatttcaa actaaaagta aaagaagctg aacgtttggg atacgacacc
    13201 atgtcgagct ttatcaaaga atataactca tacaggagcc agttggttaa accctatttg
    13261 acagataaag aaaccgatga aaaaatttta aaagaagctt acgagagaaa atgtgaagat
    13321 atccatgcca ggcaaattta ttttgccgca tccgaattag ttaagccaaa agatacgctg
    13381 gcaatatacc aaagggcaat ggaagcctac aataaactca ttagtggtgt tccatgggac
    13441 agtgtacata gtcagtattc gcaagaaaga aataaacaaa aaaaaggcga tattggttat
    13501 tttaactccc ttcagatatc ttacccgctt gaaaatatct gttttcgctt aaagaaaggt
    13561 gaaataagta aaccacaaag gacaatatat ggttatcaca tagtagaaat tctcgacaga
    13621 aggccatcta aaggagaaat cagggctgca cacataatgg tagccttccc tgaaaatagt
    13681 acccctcagt ctgtagattc ggcaaaagcc aaaattgccg aaatatacca aaaactgaaa
    13741 caaggtgaaa gctttgaaga attggccaaa caatattctg acgacaaaag aagcgccgct
    13801 aaaggcggcg atttgggatg gtttggctca ggcgatatga tcccggaatt tgaaaaaaat
    13861 gcttttgctt tggaaaaaga cagcgactat tctgctccat ttaggacatc atttggctgg
    13921 catattgtca aacgcatttc tacaaggcct gttcagcctt ttgaagcttt aaaaacatct
    13981 cttagccaaa aaattgcaaa aaatgaaaga aatgaagaag ggcgaaaagc tttaatcaga
    14041 aaaataaaag ctgaaattaa atttaaagac aacttaaaca ccgctttgtt attagtccca
    14101 tatttagata gcagtgcatt cagagggaaa tggaatgcag aaaaagcaaa gcccatcttt
    14161 gacaaagagc tattcacctt aggcaataag aaatatttca tgcaagattt tgcccaatat
    14221 ttatcttcat acaggtttaa gctgcctgcc agctacgaaa tgtctatcac ttatttttat
    14281 aataaataca tcgacgatgc aatcatagcc tttgaagact cacgcttaga atcaaaagaa
    14341 ccagagctga aatatttatt acaagaatat catgatggca ttatgctctt taaccttatg
    14401 gatcaaaaga tttggtctca agccgcaaaa gacaccgtta ttctggcaaa atattttgaa
    14461 gaccacaaaa cccagtatgt atggggacca cgtgttgaag cattggtagt atcttcgcca
    14521 gataaagagc ttgtcaataa agcttatgct tttgccaatg actttgccaa cggcaaaata
    14581 agcgctgaaa aaatttggca atcgatttgc cctgatacaa cgcaaccttg tttaaattgc
    14641 caggtcaact tattcgaaaa aggagacaac caaatattag acagcctggg ctgggacaaa
    14701 ggagttacac caattgtaaa cagagaaggt aaatttgggt tctttgtaaa aactcaacat
    14761 tttgaaggcc gcccaaaaac ttatgaagaa tctaaaggca ctgtgatagc cgattaccaa
    14821 caatttttgg aagaccagtt cgttaaagaa ctaaagaaaa aatatgctgt ggttgttaac
    14881 caaaaagtac taaaatctct tgtgaaataa ttaatccaac aaaagtttat cttctaaaaa
    14941 aaattttatg ctgaaatatt ttcttataat ttttgttttc gcaatgcctg cctggtgttc
    15001 aaaaaaagac aaacctgaag ataaaccttt ggccaaggta ggcgatactt acctatacaa
    15061 atctgagcta aatggatatt ttcgctcggg tacctccccc gaagacagta ttattatgtt
    15121 caaagccttg gtagaaaaat ggatccgcaa acaattaatc gttgaaaaag ccaaactaaa
    15181 ccttacgcgc gaagaaatgg atgttgaaaa agagcttgaa gattacaaaa cctctttgat
    15241 catttacaag tacgaacaaa aaatgctaaa agaaaagctg gacaccaatg tttctaatgc
    15301 tgaaattgaa aaatactaca atcaaaacat acaaagcttt aacctaagtt cgccaattgt
    15361 aaaagcacag tatatcaagc tttcaataaa aagcccacag ctcgacaagc ttaaactctg
    15421 gctcaaaaca gacaatcccg aaaatgtccg attgattgat gaatactgtt ttaaatacaa
    15481 tgttaagtac aattacttta acgatgagtg gataagtttt gaaaatatca aaatgatgat
    15541 gccattcacc tcaaatgtta cagaaaatta cctgtcaatg aacaagttga tagagttaac
    15601 agacagcact aacttgtatt tgttgaaaat tagggaaata aaattttcgg gaacaacagc
    15661 ccctttagaa tttgtcaagc ccaatattaa aagtatcatt atcttaaaac gaaaacaaca
    15721 actaatcaat gagcttgaag ataaaatata ctttgaagcc cttgataaaa ataaatttga
    15781 actactataa ttattaaatt cgtcataatg ttaaaaaaaa gcttatttgt tggtcttttg
    15841 ttttctttct cattgcttta cagccaggat gaaaagttag cagataaaat agtagcagta
    15901 attggtaaaa gtattgtatt gcagtctgac attgaacaac agatgcttca atataaagcc
    15961 caacgccaaa atatagagcg ttgcgaagta tttgaaaact tcctgatgca aaaaatgtta
    16021 gtcaaccaag cccgcattga cagtatcgaa atatctgagg ttaacgtaga aatgtcgcta
    16081 aataaccgta tggattattt tctgcaacaa gctggttcgc aagaaaagct ggaagaatat
    16141 ttcaacatgc cgttaaacca gattaaagat gagatgcgtg aatcaatacg cgaacagatg
    16201 cttacacaaa aaatgcaatc tgaaatcacc aaaaatatac gcattacccc atctgaagta
    16261 aaagaatttt acaagtcatt acccccagac agtattccaa tgatagacca gcaaatagaa
    16321 tacaaacaaa ttcttttata cccatctttt gccaaacagt caatatatga aacacgtgaa
    16381 aaacttttag atatcagaaa aaggatactt gccggcgaaa aattttctac cctggccgtg
    16441 ctttattcag aagatcccgg atcggttacc aatggcggcg aaattggttt catgagccgg
    16501 agcgaacttg acccggccta tgctactgcc gcattcaacc tcaaaccagg cgcagtttca
    16561 gggattgtcg aatcacagtt tggttttcat attatacaac tcatagcccg cgaaggagac
    16621 agagtaaata caaggcacat cctgataaaa cctaagataa cagcaaaaga gctccagttg
    16681 gcctttaaca ggcttgatag tattgccagg gcaataaaag ccgatagcat tacttttgaa
    16741 aaagctgcat taatatattc tgaagataaa accacccgtt ttgcatcagg acaagtagta
    16801 aacccttata ccaactctac cttgtttgag ttagaccaat tacctaaaga agatttttat
    16861 atccttagca agctcaacgt tggcgatata tctcaaccat tccagtcaaa agacgaaaac
    16921 agaaaagatg cctataaaat tgtaaaacta acaggcaaaa aagatccgca tagagccaac
    16981 ctcgatgacg attatctgtt aatacaagac ttagcccttg aatcgaaaaa aaagaaatat
    17041 gttgacgaat ggatcgaaaa taaactatca acaacatact tcagaattga cccgacattt
    17101 gactgtaatt ttaaaatcaa cgcctggaga aaaaagaatt agc
//
LOCUS       DAOSPF010000065        16824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3442_length_16824_cov_8.162323, whole genome shotgun sequence.
ACCESSION   DAOSPF010000065 DAOSPF010000000
VERSION     DAOSPF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_16824_cov_8.162323"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(692..1546)
                     /locus_tag="PK252_11630"
     CDS             complement(692..1546)
                     /locus_tag="PK252_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15401.1"
                     /translation="MKKILFIISVMFSALLQAQDSEQASVGKSGSTSDILPAAGDIAL
                     GADALPYLNYLGNMFNNTTNNTLALGKQNLYFRYYLTETTALRLILGINSTTNINKYY
                     VQDDAALSADPLSQAKLIDKENVYIKGIDINVGYMMTRGYNRLRGFYGAQVGYGFNRT
                     SFDYQYGNNLTIANSAPSTATTSQFVGGRLAKRLIEKDNGLVQNINAGLFMGVEYYFL
                     PKICIGGEVTLNFMYQWGSQSNSKYEQVVGSQIVQTDEVAAPGNKKIFLGTWRPATYG
                     GLYLMFHF"
     gene            complement(1685..2500)
                     /locus_tag="PK252_11635"
     CDS             complement(1685..2500)
                     /locus_tag="PK252_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15402.1"
                     /translation="MKHLRILSVLAGTALILASCDKDVDNSSLQIDLKKTITVKGYAF
                     AELNKRVLGLEYAPENTKVILSIPYADFNAAAGAGNWHDTVKIDANGIFTAQVPVDND
                     GVNLTIQALPFEYNQVTSYGTYEGTEKKIFEAAPVTFLITPDDYKIKQITYNAVSFDN
                     AVRQTKVNFTIKADLDESLNGAEPLQNVSLVLYTATWSQEFTTDNNGNFTAIVPLNET
                     IRVKLVYVAGKKVSVGSGYELQNYEYKLDGDGYGPFSGVSTDFTINLGGGTKL"
     gene            complement(2547..3569)
                     /locus_tag="PK252_11640"
     CDS             complement(2547..3569)
                     /locus_tag="PK252_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT15403.1"
                     /translation="MFIKSSISVLFLFVLLLNACQTPKPEQKYIKFAGETQGTTYHIT
                     CKTSDSVEVVQHNVDSILHAFDLQFSIYNKQSVISKINNNDPTVSLDSMFVDMINFSK
                     KINQMSAGAFDITVGPLVNAWGFGPDTKKRPSQKTIDSLLLITGMDKIKIEGNKLIKS
                     NPKVKIDVNGIAQGYSVDLVADFFEKSGIENYMVEIGGEVRCKGVNEKGKLWRIGIDK
                     PVENNNGAEHQLKAIVELNNESVSTSGSYRRFYVENGVKYSHTINPFTGFPARNTLLS
                     VTIFDKKCAFADAVATACMVKGLDSSKLFLSENKIKAYLIYSDLEGNLKEYFSPCLNE
                     KLFGIE"
     gene            complement(3559..4713)
                     /locus_tag="PK252_11645"
     CDS             complement(3559..4713)
                     /locus_tag="PK252_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT15404.1"
                     /translation="MFDSVALLTLQHYWWTIISLLGGVLVFLLFVQGGQTLIYTLGKS
                     DIEQSLIVNSLGRKWEFTFTTLVVFGGAFFASFPLFYSTSFGGAYWVWMSILFCFIIQ
                     AIAYEYRSKPSNFLGKRTYEIFLFINGALGTFLLGTAVATMFTGSAFSVTFENIASDG
                     NAVISSWQSSFHGLEALADWRNLMLGFAVFFLSRTLAILYFYNNIENEQVHTRAAKHL
                     IINASGFLVFFLTFVIAIFFSVGYGYTPDTKMIYLEPYKYFKNFVEMPVAGILFLAGL
                     AMVIYGLILGILKKSDKAIWYAGAGTIVTVFTLFINLGFNNTAFYPSSFDMQSSITIE
                     NGSSSLYTLKAMTLVSILVPFVAAYMFWAWKAINNKKINSSELNEDGHVY"
     gene            complement(4742..6295)
                     /locus_tag="PK252_11650"
     CDS             complement(4742..6295)
                     /locus_tag="PK252_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT15405.1"
                     /translation="MTDISLVEWSRWQFALTAMYHWLFVPLTLGLAIILGIMESIYVK
                     TGNPEWKRITKFWMTLFGINFAIGVATGIILEFQFGTNWSNYSWFVGDIFGAPLAIEG
                     IMAFFLESTFIAVMFFGWNKVSKKFHLMSTWLVAIGANLSALWILVANAWMQYPAGMH
                     FNPDTARNEMINFWDVLLSPVAINKFLHTINSGFVTASVFVVAVSAWYLLKKRETQLA
                     IRSIKIAAIFGLVSSLYIAYNGDGSAKQVAKYQPMKLSVMEGLVKGTTDAPLSILGIT
                     TKPDEKDSLEIAKVKYSIEIPGMLSFLAFNDANAFVPGIHDLIYGNKEHNILSFNEKK
                     ERGLLARNMLKELQEAKKNGNVEAYQKNREEFMSTEFQKNYFAYFGYSFIKNPSQLIP
                     SIPLIFYSFRIMVALGIFFILLFALVLWNTYKSDLGSKRWLLYIMLWSLPLPYIASQC
                     GWIVAEVGRQPWVIQDYLPTLAAVSNLNVSAVQVTFWLFAAIFTALLIAEIKIMFKQI
                     KLGPGELKK"
     gene            complement(6306..6518)
                     /locus_tag="PK252_11655"
     CDS             complement(6306..6518)
                     /locus_tag="PK252_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOT15406.1"
                     /translation="MAAVIKKVVMFYVDGFRSMTVGRKLWIIILVKLFVMFAVLKLFF
                     FKDFLGERFKNNSQQKSNYVLEQITK"
     gene            complement(6630..8774)
                     /gene="rnr"
                     /locus_tag="PK252_11660"
     CDS             complement(6630..8774)
                     /gene="rnr"
                     /locus_tag="PK252_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOT15407.1"
                     /translation="MRKNKKKEKSDSGKFYNILKGLVVEVLSSNENKALNYKQIAKKL
                     SLGDNDKREMLLAVLDELVIAGVIVEMDRGKYKIPAKSKLITGIVQMTSGSNAFVISD
                     ELEEDVLILQKNFNHALGGDKVTVNLFARRPGRRLEGEIVEILERKRKQFVGRIEAAK
                     HFAFCIVDSKDVPYDIFIPLDKLKKAKNGQKVIVKITDWPRNVKNPFGEVVEVLGAPG
                     QNETEMHAILAEYELPYKFPQEVEAEANNISDKITEEEIKVRRDFRNITTFTIDPADA
                     KDFDDALSFKKLENGNVEVGVHIADVTYYVKPGSILDKEAYNRGTSVYLVDRVVPMLP
                     ERLSNNVCSLRPGEEKLCYSAVFELDAKANVLSQWFGRTVIKSCRRFNYDEAQAIIET
                     GQGDLSEVILTLHGLAKQMRERRMKSGAIEFDRLEVKFELDEKGKPIRTYFKENKDSN
                     KLIEEFMLLANRKVAEFVGMPKHNEKPKTFVYRVHDVPNPDKLIAFARFVKKFGYSVN
                     TANEKKTASDINKLLSDVKGKNEEEVIENLALRAMAKAEYSTDNVGHYGLAFAYYTHF
                     TSPIRRYPDVMVHRLLDYYLHKNESKDAAKYEKMCKHASDMEKRATEAERASVKYKQV
                     EFMSDKIGNVYEGTISGITEWGIYVEIKDNLCEGMVPIREISDDYYFYDEENYCLVGK
                     NSNRRFQLGDNVKIKVWRTNLVKKQMDFLLVD"
     gene            8920..9684
                     /locus_tag="PK252_11665"
     CDS             8920..9684
                     /locus_tag="PK252_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOT15408.1"
                     /translation="MKKYRVSGVSYLNSIPMIHGLTQSLKVMENTELTLDTPNICAEK
                     LMTHKADIGLVPVGALLDMDYYQIISDCCIGAFGNVRTVVLASNVPIEQIETIVLDPF
                     SRTSNLLLQILAKEFWKKDFHFYKGMPEFENKDIKNTTAAVVIGDKVFSIEKKYTYII
                     DLSNEWLKFTGLPFVFAVWAATSDIDIEYISDFNQAVMQGARAVDDAIDNYPQQINIS
                     REEKASYLHHNIDFDFDEKKKTALSLFHQMAKEIKA"
     gene            9734..11134
                     /locus_tag="PK252_11670"
     CDS             9734..11134
                     /locus_tag="PK252_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT15409.1"
                     /translation="MKNKIFLILVIVGTMISNILFASASDKTRVYKFEINQSIDKSAW
                     RLTKKACEHATKINADILLLRLNTYGGLVDAADSIRTALLNFPKPVLVFIDNNAASAG
                     ALISISADSIYMRKGASIGAATVVDQEGKVVPDKYQSYMRGIMRSTAEAHGKKVVING
                     KDTTYVWHRDPKIAEAMVDPRTYIKGINDSGKVLTFTTSEAIKHGFCEGEAENIDQVL
                     SLAGIKEYEIVEYKITAMDSIIGFLLNPVVQGILIMIIVAGIYFEFQTPGIGFALFAA
                     IGAALLYFAPLYLEGLANNWEILLFIAGIILLAIEIFAIPGFGVTGILGISFILIGLT
                     MALIDNVHIKPLEYSGMVIVLRAFFLVITSVVLSFAGSLYVSGKLIKSKKTKIALSTS
                     ISSSDGFVGVDQQRLQEMKGKTGVAKTLLRPSGTVEIEGHMYDAKSETGYIEAGQQII
                     VTRSETSQLYVKPFKG"
     gene            11131..11577
                     /locus_tag="PK252_11675"
     CDS             11131..11577
                     /locus_tag="PK252_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT15410.1"
                     /translation="MNENVFNIRVYGIIFNEQRNAVLVSDEYQLNTFMTKFPGGGLDY
                     GEGTIDCLKREIIEETGHKICNIEHFYTTDFFQKALFFDNQQLISIYYTCQLEGQQNF
                     PTSTQPWKFEKAENGQERFRWNKLSELNTEQFTFPIDKIVAGMLTK"
     gene            11588..12142
                     /locus_tag="PK252_11680"
     CDS             11588..12142
                     /locus_tag="PK252_11680"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT15411.1"
                     /translation="MKVYTKTGDKGETSLVGGKRVAKSDLRLDAYGTADELLAFISLL
                     KDQQISNIHKTILSEVQDHLMTCSAILAYDFENKSVKLPSITEKEILFLEKEIDKIEE
                     TLEPLFTFIIPGGHPIVSIGHVARTVCRRAERIVVKLAEEAEVPENVIKYLNRLSDYL
                     FVLTRRLSSDLNIRESAWKPNLEL"
     gene            12197..12418
                     /locus_tag="PK252_11685"
     CDS             12197..12418
                     /locus_tag="PK252_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOT15412.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYGLRTGVSMEVIENLQEIE
                     DDGEIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            12502..15003
                     /gene="feoB"
                     /locus_tag="PK252_11690"
     CDS             12502..15003
                     /gene="feoB"
                     /locus_tag="PK252_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT15413.1"
                     /translation="MRLSDLAFGETAIIVKVFGRGAFRKRITEMGFVKGRKVTAIKSA
                     PLNDPIEYNIIGYNITLRRSEASLIEVTTIQEAEKEDFSHFNGTFTEEMLQKKAREKG
                     KIINVALVGNPNCGKTTLFNFASNSHEKVANYSGVTIDSKTAEFHYKGYQINLTDLPG
                     TYSLSAYSPEEIYVRHHIIENTPDVVINVLDSSNLERNLFLTTQLIDMDIKVVVSLNM
                     YDELSQRGDNFDYKSLGKMLGIPFIPTVSTKGIGISELFDKIINIYSEKDKTYRHIHI
                     NYGEEMEVSIRRIQEKIKQNPDITTVVSSRFLSIKLLEKDKEAEKFVKRTNNADEILQ
                     ASQKEGKRLANLYNEDSETLITDLRYGFIAGALKETYVKGKTERRKTTEFLDTLLTNK
                     ALGFPLFFIFLWVMFEATFKLGEYPKQWLEAFVSFTGQVIDKIMPSGPLHDLISDGII
                     SGVGGVIVFLPNILLLFLFISFMEDSGYMARAAFIMDKLMHRIGLHGKSFIPLIMGFG
                     CNVPAIMATRTIENRNNRLLTILINPFMSCSARLPVYILFVSAFFPEHAGTALFLIYF
                     TGILLAIGVALIFKNTIFRAEEIPFVMELPPYRMPTIRSTIKHMWFKAEQYLKKMGGV
                     ILVASIIIWALSYFPHNNDLTSVYENKAKEVEMKYNNIKVVSNTELYELDSLKNIELK
                     QLEFEKNQIHQQNSYIGKIGQFIEPAMLPLGFDWRLSVSLLTGITAKEVIVSTTGVLF
                     QAKLDDADNISLQNKLKESKFVSGPKKGETLITNLTAISFMAFVLIYFPCIAVMAAIR
                     REAGGWKWPLFVIFYTTGIAWLVSFAVFQIGSFFM"
     gene            complement(15275..15535)
                     /locus_tag="PK252_11695"
     CDS             complement(15275..15535)
                     /locus_tag="PK252_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOT15414.1"
                     /translation="MSVTLYVGNISYTMKEDELKNAFGQYGEVVSAKIIIDKRTGKSK
                     GYGFVEMATDSAAEEALTNLNGKELSGRNVKVNKANSQAEQK"
     gene            complement(15629..16627)
                     /locus_tag="PK252_11700"
     CDS             complement(15629..16627)
                     /locus_tag="PK252_11700"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT15415.1"
                     /translation="MAYNLKNRSFLKLLDFTPEEIGYLLKLSADLKAAKRCGTEQQTL
                     KGKSIALIFEKTSTRTRCSFEVAAYDQGAHVTYLSPDGSQIGHKESIKDTARVLGRLY
                     DAIEYRGFSQNTVEELAKYAGVPVWNGLTDEFHPTQILADFLTMQEHSDKPLNKISFC
                     YLGDARNNMGNSLMVGAAKMGMDFRAAAPKACQQNDELVKKCQEIAKETGAKITITES
                     VDEAVKGVDFIYTDVWVSMGEPESVWDERIKLLKPYQVNMDVIKKTGNPDVKFLHCLP
                     AFHNLETKVGKKIHEKYGMTGIEVTEEVFESKHSIVFDEAENRLHTIKAIMVATLA"
BASE COUNT         5559 a         3212 c         2943 g         5110 t
ORIGIN      
        1 agctggtttg gccaacagta ccagcatcgg aaggataaat agttgcataa attggttttt
       61 cattttggta aggtttaatt gtttaacaat aaatataaaa ttaaaaaaat ttcagattta
      121 atttgtgcgt cgaaacaaaa gcctttttaa acttttaaaa tttaataaat ggcttctaaa
      181 agatttgaaa tcaatgtcgt ttagttaggt attaaaaaaa acgctgacag gcctgaaaga
      241 cgttgtcggg ttagtttcac atctgacctg acagcgttag caaaacctgt cagagtctta
      301 actaaacgtc actctataac aaatataact tttttttttg aattttaatg ttgtttttat
      361 ggtagttatc tcagtttttt caattagctg aacccggagg ctaaaacatc atagcccttt
      421 tgggggatat ttccagcagg gttggacgtt gtaagcatat tgccaaaggc atcataagta
      481 aaacttaggc tgttaagagt cagtatgagt atttgtggag agcaattggt taaaaacaat
      541 ttgtaaatca gcttgtgctg tttatgaagg gcgggttaac ctaagtaaat aatatagttt
      601 ggcaaagtat tgcctcctaa tgtgtaattt gggataaaga aaagaggctg ccctttttga
      661 gaacagcctc ttaatgtttt atttgaaaat attaaaagtg gaacatcaaa tatagacctc
      721 cataggtggc cggacgccat gttcctaaga atattttctt attgcccgga gcggcaactt
      781 catcagtctg gactatttga gaaccaacaa cttgttcgta cttactattg ctttggctcc
      841 cccattgata catgaaattt aaagtaactt ctccgccaat acatatttta ggcaaaaagt
      901 aatactcaac acccatgaac aaccctgcat taatattctg aacaaggcca ttgtcttttt
      961 ctatcaaacg ttttgccagc cttccaccga caaactgaga tgttgtagct gttgatggag
     1021 cagagttagc aattgtaagg ttgttgccat attggtaatc aaaacttgta cggttaaaac
     1081 catagccaac ctgagctcca taaaatcctc ttaacctgtt atatccacga gtcatcatgt
     1141 aaccaacatt aatatcaata cctttaatgt aaacattttc tttatcaatc aatttggctt
     1201 gactaagagg atcggcagaa agggctgcat catcctgaac gtaatattta ttgatgtttg
     1261 tagttgagtt tatacctaaa atcaggcgta aagcagttgt ttcggtcaaa taataacgaa
     1321 aataaagatt ttgtttacca agtgctaaag tgttattagt agtgttgtta aacatatttc
     1381 ctaaataatt gaggtatgga agcgcatctg cccccaatgc aatgtctccg gcagcaggca
     1441 aaatatctga cgtactgccc gatttgccaa ctgaagcttg ttctgaatcc tgagcttgca
     1501 gcaatgcaga gaacatgaca cttataatga ataatatttt tttcatccgt aaataatttt
     1561 ttaataaggt cggataaaac acgcatgata aattttcatc cttatttgaa attaacagat
     1621 catgcacgtt tcactcgatt caattttata aataatgata atttcagctt tataaatcac
     1681 aaaactaaag ttttgtcccg ccgccgaggt tgatggtaaa atctgttgat actccgctaa
     1741 atgggccata accatctcca tcaagtttgt attcataatt ttgaagttca tagccgctgc
     1801 caacagacac ctttttacct gcaacataaa ccaatttaac acgaatggtt tcgttcaaag
     1861 gcacaatggc agtaaaattg ccattattgt cggtagtaaa ctcttgagac caggtagcag
     1921 tatataaaac taaagaaaca ttttgtaaag gttcggcacc atttaaagac tcatcaagat
     1981 cagctttaat tgtaaaattc accttagttt gacggacagc gttgtcaaaa gaaactgcat
     2041 tataggtgat ttgcttgatt ttgtaatcat caggagtaat taaaaaagta accggggcag
     2101 cttcaaaaat tttcttttca gtaccttcat aagttccata agaggtaacc tggttatatt
     2161 caaaaggtaa tgcctgtata gtaagattta cgccatcatt atcaacaggg acttgggctg
     2221 taaaaattcc attagcatca atcttaacag tatcatgcca atttccggca cctgcagcag
     2281 cattaaaatc agcataagga atgcttaata ttacttttgt gttttcagga gcatattcaa
     2341 ggcctaagac gcgtttgttt aattctgcaa aagcatagcc cttaacagta attgtttttt
     2401 tgaggtcaat ttgcaagctg ctattgtcca catctttgtc gcaagatgcc aagattaagg
     2461 ctgttcctgc caaaaccgaa agaattctga ggtgtttcat tttcattatt atttaagtta
     2521 aaggttaaat tcttcaaaat taataattat tctataccaa ataatttttc atttaaacat
     2581 ggagaaaagt attcttttaa attaccttct aagtcgctgt aaatcagata tgctttgatt
     2641 ttattttcag ataaaaatag tttggatgaa tccaatcctt ttaccataca agcggtagca
     2701 acagcatcgg caaaagcaca ttttttgtca aatattgtca cgctaagcaa ggtattacgg
     2761 gcaggaaatc cagtaaaggg attgattgtg tgcgaatatt ttactccgtt ttcaacataa
     2821 aaacgtctgt aacttcccga tgtagaaaca gactcattat ttaactcaac aatggccttt
     2881 aactggtgtt ctgcaccgtt gttgttttca acaggtttat ctatacctat tctccatagc
     2941 ttaccttttt catttacccc tttacatctg acctctccgc caatttcgac catataattc
     3001 tctattccgc ttttttcaaa aaaatcggca actaaatcaa cagaataacc ttgtgctatg
     3061 ccattcacat caatttttac ctttggatta gactttatca acttgttccc ttcaattttt
     3121 attttatcca taccagtaat gagcaacaaa gagtctattg tcttttgaga cgggcgtttc
     3181 ttagtatcag ggccaaaacc ccaagcatta accaacgggc ctactgtaat atcaaatgcc
     3241 ccggcagaca tttggttgat ttttttggaa aaattaatca tatcaacaaa catagaatcc
     3301 agagaaactg taggatcatt gttattgatt ttcgaaatca cagactgctt attatatatt
     3361 gagaattgta aatcaaaagc atgaagaatg ctatctacat tatgctgtac tacttcaaca
     3421 gaatctgagg ttttacaagt tatgtgatac gttgtacctt gtgtttcgcc tgcaaatttg
     3481 atatatttct gctcaggttt tggcgtttga caagcgttta ataataaaac aaaaaggaat
     3541 aatactgaaa tacttgattt aataaacatg gccatcttca tttaattcgg aactattgat
     3601 tttcttgtta tttatagctt tccaggccca aaacatgtat gctgcaacaa atgggacaag
     3661 tatgcttacc agtgtcattg ctttcagggt atataggctt gaagaaccat tttcgatagt
     3721 aatgctgctt tgcatatcaa aactgcttgg ataaaatgcg gtattgttga aacccaggtt
     3781 gataaacaaa gtaaatacag taactatagt acctgcacct gcataccaaa ttgctttatc
     3841 agattttttt aatatcccga gaattaatcc atatataacc attgctaaac cagctaaaaa
     3901 caagatgccc gcaaccggca tttctacaaa attcttaaaa tacttatacg gttcgagata
     3961 aatcattttt gtatcaggtg tatagccgta accaactgaa aagaatatag ctatgacaaa
     4021 tgtcagaaag aaaactaaaa aaccagaagc atttatgatt agatgttttg cagccctggt
     4081 atgaacctgc tcattttcaa tattattgta aaagtataaa atggccaagg tacgggataa
     4141 aaagaaaaca gcaaatccca acatcaggtt tctccagtct gccaatgctt caagcccatg
     4201 aaatgaagat tgccacgaag agataaccgc attgccatca gaagcaatat tttcaaaggt
     4261 tacactaaaa gcagagcctg taaacatagt agctacagca gtccctaaaa gaaaagtccc
     4321 taaagcccca ttaataaaaa gaaaaatctc gtaagttctt ttccccagaa agttggaagg
     4381 ttttgaacgg tattcataag ctatagcctg aataataaag caaaacaata tactcatcca
     4441 cacccaataa gccccaccaa agcttgttga ataaaaaagc gggaacgatg caaaaaatgc
     4501 gcccccaaaa actactaaag tggtaaaagt aaattcccat ttacgtccta aagaattgac
     4561 aatgagcgat tgttcaatgt cacttttgcc caaagtgtat atcaatgttt gtccaccttg
     4621 cacaaacagc agaaatacta aaaccccgcc taaaagagag atgatagtcc accagtaatg
     4681 ttgtaaggtc aataaagcaa ctgaatcaaa cataatcatg tgtttttagt tatttgaaat
     4741 gctatttttt gagttcccct ggcccaagtt tgatttgctt aaacataatt tttatttcgg
     4801 caatcagcaa tgcagtaaat atagcggcaa aaagccagaa agtaacctga actgccgaaa
     4861 cattaagatt agaaactgct gccaatgtag gaagataatc ttgtataacc caaggttgac
     4921 ggccaacttc agctactatc catccgcatt gactggcaat ataaggtaat ggcaatgacc
     4981 ataacataat gtacagcaac catcttttag acccgaggtc gcttttgtat gtattccaga
     5041 gtacaagagc aaacaagaga atgaaaaaaa tgcccaaagc caccattatc ctaaaactat
     5101 agaaaataag gggaatactg gggatcagct gacttggatt ttttataaag gaataaccaa
     5161 aatatgcaaa gtaattcttt tgaaactcag tgctcataaa ctcttcacgg tttttctgat
     5221 aagcctccac gttcccattc tttttagcct cttgaagctc tttaagcata ttgcgggcca
     5281 acagtccccg ttcctttttt tcgttaaaag aaagaatgtt atgctcttta ttgccgtata
     5341 tcaagtcatg gatgccagga acaaaagcat tggcatcatt gaaagccagg aaagagagca
     5401 tacctgggat ttcaatggaa tatttgacct tcgcaatttc aagcgagtct ttttcatctg
     5461 gtttagtagt aatgcccaga atacttaaag gtgcatctgt tgtgccctta acgaggcctt
     5521 ccattacaga tagtttcatt ggttgatatt tggctacctg tttggcagag ccatctccat
     5581 tatatgcaat gtacaaagac gaaaccaaac caaagattgc agcaatctta atacttctta
     5641 tagcaagttg tgtctcacgt tttttgagca aataccaggc tgaaacagca acaacaaaaa
     5701 cagaggccgt tacaaagccc gaattaatgg tatgcaaaaa tttattaata gccacaggcg
     5761 acagaagaac gtcccaaaaa tttatcattt cgttgcgggc tgtatccggg ttgaaatgca
     5821 tacctgcagg atattgcatc caggcattag ccaccaatat ccaaagggcc gaaagattag
     5881 cgccaatagc tacaagccat gtggacatca ggtgaaattt tttgctcact ttgttccatc
     5941 caaaaaacat gactgcaata aaagttgatt cgaggaaaaa ggccataatg ccttcaatag
     6001 ccaaaggcgc accaaaaata tcgcccacaa accaggaata attggaccag ttggtcccaa
     6061 actgaaactc caatatgatg cctgtggcaa ccccaatggc aaaattaatg ccaaataaag
     6121 tcatccaaaa ttttgtaata cgcttccact ctggattgcc ggttttgaca tagatacttt
     6181 ccataatgcc cagtataatt gccaaaccaa gggtcaaagg tacaaaaagc caatggtaca
     6241 tggctgtaag tgcaaattgc caacgcgacc attcaactaa actaatatca gtcatagaag
     6301 taaaattatt ttgttatttg ttctaaaacg taattgcttt tttgttgtga attgtttttg
     6361 aaacgttctc caagaaagtc tttaaagaag aacaatttta aaacagcaaa cataacaaac
     6421 agtttgacaa ggattataat ccaaagctta cggccaacgg tcatagaacg aaaaccatca
     6481 acataaaaca ttacaacttt ttttattaca gcggccatac tctgattatg tattcaaata
     6541 taacccaaaa gagctaaaaa agtttaaaac aaacggaaaa agactaaaat gatatgtcaa
     6601 gaataatgaa gtataaactt taaaaatact taatcaacaa gtaaaaaatc catctgcttc
     6661 ttgacaagat ttgttctcca aactttaatc ttaacattat ctcccaattg gaaacgacgg
     6721 ttagaatttt tcccaactaa acaatagttt tcctcatcat aaaaatagta atcgtcagat
     6781 atttcgcgga taggaaccat accttcacac agattgtctt ttatttcaac ataaatgccc
     6841 cattcggtaa tgccagagat cgttccttcg taaacatttc caatcttatc gctcataaac
     6901 tccacctgct tgtactttac agaagcacgt tcggcctcag tagccctttt ttccatgtca
     6961 gaagcatgct tgcacatctt ttcgtacttt gctgcatcct tagattcgtt tttgtgcagg
     7021 taataatcga gcaaacgatg aaccatcaca tcgggatatc gacgtatagg tgaagtaaag
     7081 tgggtatagt aagcaaaagc aagcccataa tggcctacat tatcggtaga atattcggcc
     7141 tttgccattg ctcgcaaagc aaggttctca attacctctt cctcattttt ccctttgaca
     7201 tctgaaagta acttgttgat atcgctggct gtcttctttt cattggcagt attgacagaa
     7261 taaccaaatt ttttaacaaa acgggcaaaa gcaatcaact tgtcgggatt aggcacatcg
     7321 tgaaccctgt aaacaaaagt ctttggtttt tcattatgct taggcatccc aacaaactca
     7381 gctactttgc gattggcaag cagcataaat tcttcgataa gcttgttaga atccttgttt
     7441 tccttaaaat atgtgcggat aggcttccct ttttcgtcca gctcaaattt gacctcaaga
     7501 cggtcaaatt caatagcacc acttttcata cgcctttcgc gcatctgctt ggccaaacca
     7561 tgcagtgtta gtataacttc agataaatcc ccctgaccag tttcaatgat agcctgggct
     7621 tcgtcataat tgaaacgacg gcaagatttg attacagtac gcccaaacca ttgcgataaa
     7681 acatttgctt tggcatccaa ttcaaaaact gcagaatagc agagcttttc ctcgccagga
     7741 cgtaacgagc atacattgtt tgaaagcctt tcaggcaaca taggtacaac tctgtctact
     7801 aaatatacag aagtacccct attataagcc tctttatcaa gaatagaacc tggtttgaca
     7861 taataggtaa catctgcaat atgaaccccc acctctacat taccattctc aagtttttta
     7921 aaacttaaag catcgtcaaa gtcttttgca tcagccggat caatggtaaa agttgttata
     7981 ttcctgaaat cacgtcgcac tttgatttcc tcctcagtaa ttttatcaga aatattattg
     8041 gcctcagctt caacttcctg aggaaacttg tatggcaact cgtactcagc aaggatagca
     8101 tgcatctctg tttcgttctg cccaggagcg ccaagcactt caactacttc tccaaatggg
     8161 ttctttacat tgcggggcca atcagtaatt ttaacaatca ccttctgtcc attcttagcc
     8221 tttttaagtt tatcaagcgg gataaatata tcgtaaggga catccttact atctacaata
     8281 caaaaagcga agtgtttagc agcttctatg cgcccaacaa actgcttgcg cttacgttca
     8341 agaatttcaa caatttcacc ttccaaacgt ctcccgggac gacgtgcaaa caaattgacc
     8401 gtcaccttat cgccgccaag tgcatgatta aaatttttct gcaaaataag cacatcttct
     8461 tccagctcat cgctaataac aaaagcatta ctgcccgatg tcatttgcac aataccggta
     8521 ataagttttg atttggcagg aattttgtac tttccccgat ccatctccac aataacacca
     8581 gcaatgacaa gctcatccag cactgcaagt agcatttccc ttttgtcatt gtcccccaat
     8641 gaaagctttt tggcaatttg tttataatta agggctttat tctcattgct tgataaaacc
     8701 tcaacaacga ggcctttcaa tatattatag aattttcctg agtcagattt ttcttttttc
     8761 ttgttttttc tcatacataa taatttgaac acaaatgtaa tataattaat tgaggaatgg
     8821 ctacaaatgg cacagaagca atcctttcca tgttttcaaa caaaaaaacg tggggtttgt
     8881 tagtttgctt gttaaatttg cagccaatta ataaatatca tgaaaaaata cagggtttca
     8941 ggagtttcgt accttaactc aataccaatg atacacggct tgacacagag cctgaaagtg
     9001 atggaaaata ctgaattaac attagatacc cctaatattt gtgcagaaaa actgatgacc
     9061 cataaggccg acattggatt agtaccggta ggagccctat tagacatgga ttattatcag
     9121 ataatttctg attgctgtat tggtgctttt ggaaatgtca gaacagtagt tttagcaagc
     9181 aatgtaccta tagaacaaat agaaacaata gtattagacc cattttcacg aacatccaat
     9241 ttgcttttgc agattcttgc taaagaattt tggaagaaag attttcattt ctacaaagga
     9301 atgcccgaat ttgaaaataa agatattaaa aatactactg ctgctgtagt tattggcgat
     9361 aaggtttttt ctatcgaaaa aaaatatacg tatattattg atctttccaa tgaatggctc
     9421 aaatttaccg gcttaccttt tgtttttgca gtatgggcag ccacgtcaga tatcgacatt
     9481 gagtacattt cagatttcaa ccaagctgta atgcaaggtg caagggctgt tgatgatgct
     9541 atcgacaatt atccccagca aataaacatt tcaagagaag aaaaagcgag ctaccttcat
     9601 cataacatag attttgattt tgatgaaaag aaaaaaacgg cattaagcct gttccatcaa
     9661 atggccaagg aaattaaagc ctgaaaaatc aattattttt gttattttag cttttactgt
     9721 taaaaataaa aaaatgaaaa acaaaatatt cctcatctta gttattgttg gaacaatgat
     9781 ttcaaacatt ttatttgcat cagcttctga taaaaccagg gtttacaagt ttgaaatcaa
     9841 ccaatcaata gataaatcag catggagact gaccaaaaaa gcttgtgaac atgcaacaaa
     9901 gatcaatgca gatattttac ttctgcgtct caatacttat ggtggtttag tagatgcagc
     9961 cgactctatc cgaactgcat tattgaactt tcctaagccg gtattggtat ttatagacaa
    10021 taacgcagct tcagccggag cgctcatatc aatatcagca gatagtatat atatgcgaaa
    10081 aggggcaagc ataggtgctg ctaccgttgt cgatcaagaa gggaaagtag tgccagacaa
    10141 gtatcaaagt tacatgcggg gtatcatgcg ctcaacagct gaagctcatg gcaaaaaagt
    10201 agtaatcaat ggaaaagaca caacttacgt atggcatcgc gaccccaaaa ttgctgaagc
    10261 catggttgac ccgagaactt acatcaaagg catcaacgac tcagggaaag tactgacttt
    10321 tacaacttca gaagccatca aacatggatt ttgcgaaggt gaagctgaaa atattgatca
    10381 ggttctttct ttagctggca ttaaagaata tgaaatagtg gaatataaaa taacagcaat
    10441 ggattcaatt attggattct tgttgaaccc cgtagtgcaa ggaattctga ttatgatcat
    10501 tgttgccggc atatatttcg agttccagac gccaggcata ggatttgcac tgttcgctgc
    10561 cattggggca gcattgcttt actttgcccc gctttacctc gaggggcttg ccaacaattg
    10621 ggaaattttg cttttcatag ctggtataat tttgttggca atagagatat ttgccattcc
    10681 cggatttgga gtcacgggta tactcgggat aagttttatt cttataggcc ttaccatggc
    10741 acttatagac aatgtacaca ttaagccatt agaatattcg ggtatggtaa tagtattaag
    10801 ggcttttttc cttgtcatta cttctgttgt tttatccttt gccggttcat tatatgtcag
    10861 tggtaagcta atcaaatcga aaaagacaaa aatagcttta tcaacaagca ttagcagctc
    10921 agatggattt gtaggcgttg accagcaacg tttacaagaa atgaaaggta aaaccggtgt
    10981 agcaaaaaca ctactaaggc cttccgggac agtagaaatt gaaggccata tgtacgatgc
    11041 taaatctgaa acaggatata ttgaagccgg ccagcaaatc attgtcaccc gttctgaaac
    11101 aagtcaattg tatgtaaaac catttaaggg atgaatgaaa acgtttttaa cataagggtt
    11161 tacggcatca ttttcaatga acagcgcaat gcagttttag tttcagacga ataccaactc
    11221 aacaccttta tgacaaagtt tcctggaggt ggattagatt acggtgaagg gacaatagac
    11281 tgtctaaaaa gggagattat tgaagaaacc ggtcataaaa tctgtaatat tgaacatttt
    11341 tacactaccg acttttttca aaaagcacta ttttttgata accaacaact tatcagcata
    11401 tactatactt gtcaacttga ggggcaacaa aactttccaa catccacgca gccctggaag
    11461 tttgaaaaag cagagaatgg ccaggaaagg ttccggtgga acaaactcag cgaactgaat
    11521 acagaacaat ttacgttccc aattgataag atagttgctg gaatgcttac caaataacat
    11581 ttacaagatg aaagtatata caaaaacagg agataaaggc gaaacaagcc ttgttggagg
    11641 taaaagggtt gccaaatcag accttcgtct tgacgcttac ggaacagccg atgaattatt
    11701 agcttttata agccttctga aggaccagca gataagtaac atacacaaaa ccatattatc
    11761 agaagttcag gatcatttga tgacttgctc agcaatactg gcttatgatt tcgagaataa
    11821 gtcagttaaa ctcccttcca ttactgaaaa agagatatta tttcttgaaa aagaaattga
    11881 caaaattgaa gaaaccttag aacctctttt tacgtttatt attccaggag gacatcctat
    11941 tgtttcaata ggccatgtag ctcgaacggt gtgcagaagg gcagaaagga ttgtagtaaa
    12001 acttgcagaa gaagctgaag tacctgaaaa tgttataaag tatttaaatc gactgtcaga
    12061 ctatctattt gtattgacgc gcagactttc gtccgattta aatatcagag aatcagcatg
    12121 gaagccaaat ttggaattat agaaaatatt tttaattttt gcacaaaaat ttaaaaagcg
    12181 taaaaaccat caaaatatgt attggacgtt agaattagct tcaaaattag aagatgcacc
    12241 ttggccagca actaaagaag aacttataga ttatggcctt cgtaccggag tttctatgga
    12301 agtgatagaa aaccttcagg aaatcgaaga tgacggggaa atctatgaaa gtattgaaga
    12361 tatctggcca gattatccca gtaaagacga tttcttcttt aatgaagatg aatattaaca
    12421 gaaagtcccg atatgcggga cttttttgtt ataaatattt gaacaaacac tattttcttt
    12481 tgttaggata aaaaaataac catgcgatta tcagacttag cttttggcga aaccgcaatc
    12541 attgtaaaag tatttggacg aggagctttt agaaaaagaa ttacagaaat gggatttgtc
    12601 aaaggccgta aagttaccgc tatcaaatct gcccctctaa atgaccctat tgaatacaat
    12661 attataggat acaatattac acttcgtcgt agtgaagcat ctttaataga agttaccacc
    12721 atacaagaag ctgagaaaga agatttctcg cacttcaatg gtacttttac agaagaaatg
    12781 ctgcaaaaaa aagcccgtga aaaggggaag ataataaatg tagcccttgt aggaaacccc
    12841 aactgcggaa aaacaacact gttcaatttt gcatccaatt cgcatgagaa agttgccaat
    12901 tatagtgggg ttacaattga ctcaaaaacc gcagaatttc attacaaagg atatcagata
    12961 aacttaacag acttacccgg gacatactca ctctcggcat attcacccga ggaaatttat
    13021 gtaaggcacc atatcattga aaatacgcct gatgtagtta tcaatgttct tgattcttct
    13081 aaccttgaac gaaacttatt tttgacaacc caactaatag atatggacat taaggttgtg
    13141 gtatcgctga atatgtacga tgaactttcg cagcgtggag ataactttga ttataagtca
    13201 ttaggcaaaa tgcttggtat cccttttatc ccaactgtaa gtaccaaagg gataggcatt
    13261 agtgaacttt tcgataaaat tatcaatata tattcggaaa aagacaaaac ctaccgtcat
    13321 atccatatta actatggcga agaaatggag gtttcaattc gacgtatcca ggaaaaaatc
    13381 aaacaaaatc ccgatattac aacagttgtc tcgtctcggt tcctatccat taaactgctt
    13441 gagaaggata aagaagccga gaagtttgta aaaaggacaa ataatgcaga tgaaatttta
    13501 caggcctcgc aaaaagaggg caagcgtttg gctaatttat ataacgaaga cagcgaaaca
    13561 cttatcacag accttcgtta tgggttcatt gccggagctt taaaagaaac ttatgtcaaa
    13621 gggaaaaccg aaaggaggaa gacaacagaa tttttagaca ctttgcttac caacaaagct
    13681 ttgggcttcc cattgttttt tatattcttg tgggtaatgt ttgaagctac attcaagctg
    13741 ggcgaatatc ccaagcaatg gcttgaagcc tttgtatcgt ttaccggaca agtgattgac
    13801 aaaattatgc cttcaggtcc attacacgac ctcattagcg atggtatcat cagtggagtt
    13861 ggaggagtca tcgtattttt gcctaacatt ttattgctat tcctgtttat ttcttttatg
    13921 gaagactcag gatatatggc acgagcagca tttatcatgg ataaacttat gcaccgcatt
    13981 gggcttcatg gcaaatcgtt tatccctctc atcatgggct ttgggtgcaa tgtgcctgct
    14041 ataatggcaa cacgtactat tgaaaaccgc aataacaggc tacttactat acttataaac
    14101 ccatttatgt cgtgtagtgc gcgactgcca gtttatatct tatttgtctc tgcttttttt
    14161 ccagaacatg cagggacagc attattcctg atttatttta cagggatatt attggccatt
    14221 ggagttgcct taatatttaa aaacactata ttcagggcag aagaaatacc ttttgtcatg
    14281 gaacttcctc cataccgcat gccaaccata cgctccacca taaaacacat gtggttcaaa
    14341 gctgaacagt atttaaaaaa aatgggaggc gtaattctgg tagcttctat catcatttgg
    14401 gctttaagtt atttccctca taacaatgat cttacttcag tttacgaaaa caaagctaaa
    14461 gaggtagaga tgaagtataa caacattaaa gttgtctcca atactgaatt atacgagctt
    14521 gactctctaa aaaacataga gttaaagcaa ttagaatttg agaaaaatca aattcatcaa
    14581 caaaattcat acattggaaa gattggacaa tttattgagc ctgcaatgct gccattaggg
    14641 ttcgattggc gtttaagtgt cagcctgctt acgggtatta cagccaaaga agtaattgta
    14701 agtaccactg gtgttctttt tcaggcaaaa ttagatgacg ccgacaatat atcgcttcaa
    14761 aacaaactga aagaaagtaa gtttgtatct ggccctaaga aaggcgaaac acttattacc
    14821 aatcttacgg ctataagttt tatggcattt gtgctgattt actttccatg tattgctgta
    14881 atggcagcaa tcaggcgcga agccggagga tggaaatggc ctttgtttgt tatattttac
    14941 acaacaggta ttgcatggct tgtttcattt gcggtatttc agattggaag tttttttatg
    15001 taatggaatc aagattaaaa aaccaagaga caagaagaag ataatttaaa aacatgaaat
    15061 acatatctga ttaattatga actggcagga aattataaca tatctcatta ttgcagcatc
    15121 tgtaatatat gtaattgtac agctttatca ttcgttcttt tcgaaaaaag ctaaagataa
    15181 atgttgcacc tgcaatgtcc aatgtaatat aaaacaatag ggagaacctt tcgtcctccc
    15241 tatgatactt aaacttaaaa caggatataa taacctattt ttgttctgct tgcgaattgg
    15301 ccttgtttac tttaacattt ctgccactaa gttctttacc attaaggtta gtaagagctt
    15361 cttctgctgc agaatcagta gccatttcaa caaaaccata ccctttagat tttccggtac
    15421 gtttatcaat gataattttt gctgaaacaa cttcgccata ctgaccaaaa gcatttttca
    15481 gttcatcttc tttcattgtg taagagatgt tgccaacata aagtgttacg ctcataagaa
    15541 agtttttaga aattaagact atttaacaaa tataatctaa tttcgcaatc aaacaatatt
    15601 tttcgataaa aaatcgtttg ctctcttttt atgccaaagt agctaccata atagctttta
    15661 tagtatgtaa gcgattttca gcctcatcaa agacaataga atgttttgat tcaaaaactt
    15721 cttctgttac ctcaatccct gtcataccat atttttcatg aattttcttt ccaactttgg
    15781 tctcaaggtt atgaaatgct ggtaaacagt gcaaaaactt aacatccgga ttgcctgttt
    15841 ttttgataac atccatattt acctgatatg gcttcaacag cttaatacgc tcatcccata
    15901 ccgattcggg ttcacccatt gatacccaaa catctgtata aataaaatca acccctttta
    15961 cagcttcgtc aacactttct gtaatggtta tttttgcacc ggtttctttg gcaatctcct
    16021 gacatttctt aaccagctca tcattttgct gacaagcctt aggtgcagca gcacgaaagt
    16081 ccatgcccat ctttgcagcc cctaccatta gtgaattacc catgttgtta cgggcatcgc
    16141 caagataaca aaaactgatt ttatttagtg gtttatctga atgttcttgc atagtaagaa
    16201 agtctgccaa tatttgagtt ggatgaaact catctgtcaa accattccaa acaggcacac
    16261 cagcatattt ggctaattct tcaacagtat tttgcgaaaa gccccgatac tcgattgcat
    16321 cgtacaatct gcctaaaacc cttgccgtat ctttaataga ttctttgtgc cctatctggc
    16381 tcccgtccgg acttaagtat gtaacatgtg ctccttgatc ataagctgcc acttcaaaag
    16441 aacaacgggt acgagttgat gtcttttcaa atattaaagc aatacttttc cctttcaaag
    16501 tctgctgctc agtaccacaa cgttttgctg ccttaagatc ggcagacagt tttaataaat
    16561 atcctatttc ttcgggagta aaatcaagca gtttaagaaa actgcggttt ttaagattgt
    16621 aagccataat gttactttta aaatttaaac ggtaaagtta tttatttata gtaaattatc
    16681 gaatcaagac tttttatttt ctgtaggcag tttttatcaa tagtcagtat ttttcaaaaa
    16741 aaaagcgtga tgtacttaca ccacgcttcc tcccgacttt gacggtttga ttttataaaa
    16801 caaagatatt caaatagtta ttat
//
LOCUS       DAOSPF010000066        16778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3453_length_16778_cov_7.097590, whole genome shotgun sequence.
ACCESSION   DAOSPF010000066 DAOSPF010000000
VERSION     DAOSPF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16778
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3453_length_16778_cov_7.097590"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..610
                     /locus_tag="PK252_11705"
     CDS             2..610
                     /locus_tag="PK252_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOT15416.1"
                     /translation="MRKYWILLTLFFVGLVVSAIFPHDYFTWFLEVFPAILALIALVL
                     TFNKFRFTTFTYSFIVLHCYILFIGGHYTYAEVSLFDWIRDVFHQSRNNYDKVGHFMQ
                     GFVPAMVIRELFLRNKIVKAGGWLGFVTISVCLAISALYELFEWFVAVASGRAADAFL
                     GTQSYVWDTQSDMLFALIGATSMILLFSKVQDKQINKLGKVQ"
     gene            684..974
                     /locus_tag="PK252_11710"
     CDS             684..974
                     /locus_tag="PK252_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOT15417.1"
                     /translation="MRRSQTLPVGDLLREYLSKYGLEQKFLEAKVMYSWPEVVGTAVA
                     KKTKNLAIKNRVLFVYLESSVVKNHLGMIKNDLPALLNEKAGFSVIDEVVLR"
     gene            1007..4369
                     /gene="mfd"
                     /locus_tag="PK252_11715"
     CDS             1007..4369
                     /gene="mfd"
                     /locus_tag="PK252_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT15418.1"
                     /translation="MDFSALYQQKHPRFLDFISSIKNKPNARAKVSGIAGSSRSAICS
                     AFFKEINTSCVFLLNEYEDAAYFASDLLNYFEEDSIVFFPSSYRRPGHSIQEDASHII
                     RRTEALKRLTLSADKPLLVVTYPEALAERVGEAGQLKKSMITLHSNEKVDLAFLKETL
                     KELGFERSDFVISPGQYSVRGSIVDVYSFSAKFPYRIDFFGNEVDSIRSFDLETQLSV
                     DKLDKIDILPDLKKNSGKGNMVSFFSLLPPDSVVWAESLTDTFRQVNELQKGHWIGLK
                     PEEDGRLPIVDYLVDEESLIRSIQPFKIIETSSVSFFKSEIHFEFSIRHQPNLNKNFD
                     LLVSSVKQYKQDGYEVYICSDQPKQLVRIEDIVKSIDKEAAFVPLEQSVHEGFIDEDL
                     KIAVFTEHQIFGRYQKIRYRNDFAANQTFTLSEITDLHPGDFIVHIDHGVGRFGGIEK
                     VEINGHWQEVIKLVYRDNDVLYVSIHSLHRISKYKGKDGEPPSVHKLGSGAWQKTKAV
                     AKSKVKDIAKDLIALYAKRKESPGFAYSPDSFMQEELEASFIYEDTPDQEKATMLVKQ
                     GMERDYPMDMLVCGDVGFGKTEIAIRAAFKAVADNKQVAVLVPTTILALQHFKTFSER
                     LKGFPCKVDFISRMRKPKDIREILKKLKEGDINILIGTHKLLSKDIEFKDLGLLIIDE
                     EQKFGVAMKEKLKQLKLNIDTLTLTATPIPRTLQFSLMGARDLAIINTPPPNRHPIVT
                     EVHSFNETIIREGIMYELMRGGQVFFLNNRIANLSDLQMVIRKLVPEARTVIAHGQMD
                     PEKLEEIILDFIDGQYDVLIATAIIESGIDISNANTIFINDAHHFGLSDLHQLRGRVG
                     RSNKKAFCYLLTPPVTTLTSEARRRLKAIEDFSELGSGFSIAMQDLDIRGAGNLLGAE
                     QSGFIAEIGYETYQRILNEAIQELKESDFKDLFYNEKTETQIAQPSSNVLNNVMYITD
                     CQIDTDLEILIPEVYVESITERIKLYRELDNLQTDEDLAAFVSRLADRFGPVPQSCTD
                     LIDVVRLRRMALQLGFERIVLKNKQMVIYFIANQKSPYYQSPVFIGVLNAVNKNPKVF
                     RMKESKDKLIMSVDNISKVGQAIDVIRKLNMD"
     gene            4434..4580
                     /locus_tag="PK252_11720"
     CDS             4434..4580
                     /locus_tag="PK252_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15419.1"
                     /translation="MTKKQEKELVASFKEYSKEILSSKENSQKFLIELGICTKNGNLT
                     HNYR"
     gene            4708..5190
                     /locus_tag="PK252_11725"
     CDS             4708..5190
                     /locus_tag="PK252_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15420.1"
                     /translation="MYFWEYNYERALDYAKLIHKNPVRSKNKIEKPSVLGAVIDLGHC
                     LDLLDSAYLNIVKEAYKLLEKSSKNAGFELPKNLSIENEKDILIRKLDCAVIEAVHHY
                     HREKNIVFDSVRNVFFEGDCLYENAGFREKNHIQICVRNPNCIKGFFIPRNHIENWDE
                     "
     gene            5265..5717
                     /locus_tag="PK252_11730"
     CDS             5265..5717
                     /locus_tag="PK252_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15421.1"
                     /translation="MRILFFIGIIFPCTMLTSQELPISHVIESSSISTIEEEMFCNDS
                     SIVLTNDSMAVMCSNLNANKNSSFATVLIKAGYGLFLAGGIVLCAGFIGGWMISGLVI
                     LTQMPYLAYEIIAIFSGYILYRIGKRIELNFIINHNRGKALNVNLQKY"
     gene            5810..6550
                     /gene="pgl"
                     /locus_tag="PK252_11735"
     CDS             5810..6550
                     /gene="pgl"
                     /locus_tag="PK252_11735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT15422.1"
                     /translation="MRFSDDILHIAPSPIKLAEELAGWFYAQVTAHLEQNPNIYIAFS
                     GGNSPIMFFRNIAEKYGKKIEWQRVHIFWADEKCVAPDHYDSNYYCANKHLLSRIEIP
                     EANIHRIHGENTDNEPKRYAHEILSIVPKYQEIPCFDLFFLGMGDDGHIASIFAGQNN
                     VIFSGNIVEPSVDPRKDQKRITITPYCINNSKKIIFNVSGLAKSAIVDAVINKKADQL
                     ELPGLQIRPALGEMYWFFDIGAAQKLFV"
     gene            6554..7894
                     /locus_tag="PK252_11740"
     CDS             6554..7894
                     /locus_tag="PK252_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease family protein"
                     /protein_id="HOT15423.1"
                     /translation="MKTSIGNIILDIENQWKQELYGHCAAIFKTCHIPSHDQTHHARV
                     WNYASSLAVSLYFRGYPVDKPFLTKLIICCFFHDTGLSVTLDASHGKASANICKEYFK
                     NIAINGFEEILRAIEFHENKDDKKLSLKKNLLGSLLTIADDLDAFGYTGIYRYAEVYS
                     LRGIDIAEISSSVLINAKARYQRIVKSLPADVLKTDVLYNNYRELGRFFDVSNSDNVT
                     TLVNTIKSNIIANKQQLNSIVESQQAGLFKDLLVKIEEELNKNLDSSNIVCFSKNEIT
                     ASDGKINSYSLASALILVLFVVCPFSLWYEDVSLKESLFNVAKTVYIWFIPLVIVHEG
                     LHALFLILTNKFNVKALKFGFSKTSLSPYTHYNQVVTKVSYLVSLFAPFVLIGIIPLF
                     ISAFTGSGYWFLLSIISIWTCTGDLLAVFLLSKVPNQYFVLDHTERLGFFVIER"
     gene            complement(7891..8505)
                     /locus_tag="PK252_11745"
     CDS             complement(7891..8505)
                     /locus_tag="PK252_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT15424.1"
                     /translation="MKIFCLANNFAQHAQEIQHEIPEKPVFFLKPDTALLRNNEPFPI
                     PSFSNEVHYELELVLKIKKLGKSIKPQFASRYYSEVGLGIDFTARDIQRECKAKGLPW
                     EIAKGFDYSAAISSQFIEISALPYPQNIHFWLEINGKVVQNGYSSDMLFKFDIILSHI
                     SQYMTLRTGDLIYTGTPPGVGCVHPGDRLKAYLEDQLLMDFRVV"
     gene            complement(8502..9368)
                     /locus_tag="PK252_11750"
     CDS             complement(8502..9368)
                     /locus_tag="PK252_11750"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT15425.1"
                     /translation="MFDIEHPIPSYLTSRKNISRLVGFTALFALVFINIYDPFGAKYW
                     LEASEYKLLLMSSLVILTGVFVVVVSRLIMFYYYKKHSLLFWQYGTWIFVEIFVMAMF
                     YALFIKFGLKDGRILFELIKKSLQTTALIILLPYSVMWLYFSYQNKKEQIDAMTNNFF
                     KREQAVQPQGMLPFYDEKGVLRISIKIENLLYIEASDNYVNLYYINSKQKVSRFLLRN
                     NLKKIEPELKMQNIVRCHRSFMVNSQRVKVLRKEKDGLKIELDTPELVEIPVSKTYSE
                     NIMQILLKDNHL"
     gene            9537..10847
                     /locus_tag="PK252_11755"
     CDS             9537..10847
                     /locus_tag="PK252_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT15426.1"
                     /translation="MKTPIDTAIVAKAIDESKVHNVQRASIREVVRLVNTIEKLSGTK
                     FVRMEMGVPGINAEKVGVEAEIESLKRGVASIYPDIEGIKELKDEASRFIKLFLDINI
                     SPSGIVPTVGSMQGGFCAFLMCARRDVNKTTTLFIDPGFPVQKQQHKVLGIPVEQFDV
                     YNYRGEKLAEKLESYLKTGKIASIVYSNPNNPSWICFTDDELKTIGTLAKKYDVIVIE
                     DLAYFGMDFRQDYSVPGQAPFQPTVAKYTDNYMLMISSSKIFSYAGQRIGILAISDTL
                     YHRTFPDLKRYFTSDSFGFAAIYGALYALSSGTCHSAQFALAAMFKAVNDGAANLTAS
                     VKEYEKRASLMKELFVKYGFNIVYDTDLGRPLANGFYFTISYPGYTGGELVEELLPYG
                     ISAITLDITGSERKEGLRACVSQVRMEQIPVLEERLKIFYENHK"
     gene            10860..11075
                     /locus_tag="PK252_11760"
     CDS             10860..11075
                     /locus_tag="PK252_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT15427.1"
                     /translation="MAKGAIVVDKDKCKGCEVCVGACPQHVIAMQKDVNRKGYHYAYM
                     KNPDACTGCSNCAIVCPDGVITVYRAK"
     gene            11102..12172
                     /locus_tag="PK252_11765"
     CDS             11102..12172
                     /locus_tag="PK252_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT15428.1"
                     /translation="MSEIRLMKGNEAIAEAAIRAGCDAYFGYPITPQSEVMEYLMAER
                     PEKRTGMIVLQAESEVAAINMVFGAAAAGKRVMTSSSSPGISLKLEGISYIAGADLPC
                     VILNVARGGPGLGTIQPAQSDYFQAVKGGGHGDYHMLVLAPASVQEMADHMPLAFDLA
                     FKYRTPVMILSDGVIGQTMEKVKLFDQMPRSTEVPQWAVCGNAGRKNRIITSLDLDPV
                     KKEQQNLALQAKYQQMRENEVRYEKYLCDDADYLMVAYGASARIAKKSIEYAREKGIK
                     VGLLRPITLFPFPSAVIAELAKKMKGILSVEMSAGQMVEDVLLATRQTPQVPVKHFGR
                     YGGVVHSPEEIFEAFLKHFNLG"
     gene            12174..12953
                     /locus_tag="PK252_11770"
     CDS             12174..12953
                     /locus_tag="PK252_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT15429.1"
                     /translation="MNVNDIIKPENLVYSKTSLMNDRELSYCPGCGHGTANRLVHEVI
                     EEMGIGGETIGVAPVGCAVMLYEFSNLDFVQAAHGRATAVATGLKRVMPDKFVFSYQG
                     DGDLAAIGTGETLHTCNRGENITIIFINNGIYGMTGGQMAPTTLVGMKTSTSPYGRDE
                     KWMGSPIDMTKLVSMLPGTYYVTRQAVHTPSAVRKAKKAIQKAFEVQKNKLGLSFVEI
                     VSNCNSGWKMSPVKANQWMEENMLPYYPLGDLKVDGKLITK"
     gene            13053..13589
                     /locus_tag="PK252_11775"
     CDS             13053..13589
                     /locus_tag="PK252_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT15430.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDMEVSWMPSYGPEMR
                     GGTANVTVILSDKEISSPVLTRFDTAIILNQQSLDKFEQSVKPGGILLYDNNGISVKP
                     SRTDIKIHVVEATAEAARMGSPKVFNMMVLGAYLKLRPIVTVDNVIAGLKKSLPARHH
                     HLLDENKAALIRGMEIVA"
     gene            complement(13743..15872)
                     /locus_tag="PK252_11780"
     CDS             complement(13743..15872)
                     /locus_tag="PK252_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOT15431.1"
                     /translation="MNAKHVEKISAQMGINAENVENTISLLEQGATIPFISRYRKEMT
                     GSLDEVQVAEIKRLMEKLIEIDNRRETILNSIEEQGKLSDELRSKIEACSTLSELEDL
                     YLPYKPKRRTRATIAKEKGLEPLAGTIMKQQTVNIYGFAEKFINKDVANADEAIAGAT
                     DIIAEWVNENVRARNQLRKLFRHEALIISKVVKGKEPDAIKYGDYFEFSEPLKRCPSH
                     RFMAMHRGEEEGFLKISIEPAQEKAIAMLEEIFIKADNECSDIVKKAITDSYKRLLGP
                     SIETEIKGEAKEKADDEAIRVFSENLRQLLLAPPLGQKQTLAIDPGFRTGCKVVILDN
                     QGKLLHNETIYPHPPQNERGKAAAKINALVNTYNVEAIAIGNGTASRETENFIKTVRF
                     ERDIKVYIVNESGASVYSASQVARDEFPEYDVTVRGAVSIGRRLMDPLAELVKIDPKS
                     IGVGQYQHDVDQGKLKKSLDQVVELCVNSVGVELNTASKQLLSYVSGLGPALAAAIVA
                     YRNENGAFESRKQLMKVPRLGEKAFEQCAGFLRIRNAHNPLDSSAVHPESYPIVEKMA
                     ADVGTNVEQLIKDVELQKKIDIKRYVTAKTGLPTLQDIMQELSKPGRDPRTIIKVFEF
                     DPNVQTVNDLKEGMVLPGIVTNITRFGCFVDVGVHQDGLVHISHLANRFVSDPNEIVK
                     LHQHVRVKVIEIDKVRNRIQLSMKEVE"
     gene            complement(16031..16489)
                     /locus_tag="PK252_11785"
     CDS             complement(16031..16489)
                     /locus_tag="PK252_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT15432.1"
                     /translation="MGRGNESFGKKEVRAKKEKKRKEKEQRRQERKGQSRDGNNLDDM
                     IAYVDEFGNITSTPPDPTRKTVINAEEIEIGVPRDNSPKVNETIRSGIVTFFDTSKGF
                     GFIKDSASGQDIFVHAKSLSAAIAENDIVTFEVIKGPKGPNAVNVAIVKE"
BASE COUNT         4765 a         3171 c         3555 g         5287 t
ORIGIN      
        1 catgcgtaaa tactggattt tattaacctt gttttttgta gggcttgttg tttctgccat
       61 ttttcctcac gattatttta cctggttttt ggaagtgttt cctgctattt tggctttaat
      121 tgctcttgtt ttaactttca acaagttcag gtttaccaca tttacttact cttttattgt
      181 gttacattgc tatatcctat tcattggtgg acattacacc tatgctgagg tttcattatt
      241 tgattggata agggatgtat ttcatcaaag ccgcaacaac tacgataaag ttggccattt
      301 tatgcaaggt tttgttcctg caatggtcat aagggaattg tttcttcgaa ataaaattgt
      361 taaggcagga ggctggcttg gctttgttac tattagtgtc tgccttgcta ttagcgcctt
      421 atatgaactt tttgaatggt tcgtagctgt tgcttcaggt cgggctgccg atgcatttct
      481 tggaacacaa agttatgttt gggatacgca gtctgatatg ctttttgctc tgataggggc
      541 aacctccatg attcttttat tttctaaagt gcaggataaa caaattaata agcttggcaa
      601 agttcaataa tggatatttt gcatttcatt aattcgctca ttcacaaatt ttcaaatttt
      661 caaattttca aattattatt tttatgcgca gaagccaaac attgccagta ggggatttgc
      721 tcagggaata tctctcgaaa tatggattag agcagaagtt ccttgaagct aaagtcatgt
      781 attcgtggcc agaagtggtt gggactgctg tggctaaaaa aaccaagaac ctggccatta
      841 aaaaccgcgt attgtttgtt taccttgaat cttcggttgt gaaaaaccac ctgggcatga
      901 taaaaaatga ccttccggcc ttgctgaacg aaaaagcagg tttttcggtg attgatgaag
      961 tagtattgcg atagtttttg cacttttgca tgtcaaattt ttcgcaatgg atttttcggc
     1021 tttatatcag caaaaacacc cccgttttct tgattttatt agtagtataa aaaataagcc
     1081 caatgcacgt gccaaagtta gtggcattgc agggtcttcc cgttcggcta tctgctcagc
     1141 tttttttaaa gagataaata catcttgtgt tttcctgctc aatgaatatg aggatgctgc
     1201 atactttgct tcagatttgc tcaattattt tgaggaagac agcattgtct ttttcccttc
     1261 gtcttatcgc aggccgggtc acagcattca ggaagatgca tcgcacatca tacggcgtac
     1321 agaagcttta aaacgcttga ctttgtctgc tgacaaacct ttgcttgttg ttacttaccc
     1381 tgaggccctg gccgaaagag taggagaggc cggacaactc aaaaagtcta tgattaccct
     1441 gcatagcaac gaaaaggttg acctggcatt tttgaaagaa accctgaaag agcttggctt
     1501 cgagcgtagc gattttgtta tcagtcccgg gcaatattcg gtgcgtggga gtattgttga
     1561 tgtttattct ttttcggcca aattccctta tcgtattgat ttttttggca atgaagtgga
     1621 ctctatccgc tcgtttgacc tcgaaacaca gctttcggta gataagctcg ataaaattga
     1681 tattctgcct gacctgaaaa agaactcggg caaaggaaac atggtctcgt tcttttcttt
     1741 attgcctcca gatagtgtgg tatgggccga aagccttacc gatacattcc gacaggtaaa
     1801 tgagttgcaa aaagggcatt ggattgggtt gaaacctgaa gaggatggtc gtttgcctat
     1861 tgttgattat ttggttgacg aagaaagcct tattcgaagc attcagccgt tcaaaatcat
     1921 agaaactagt tctgtcagct ttttcaagag tgagatacat tttgaatttt caatacgcca
     1981 ccaacccaac ctgaacaaaa attttgattt gttggtatct tctgtaaagc aatataaaca
     2041 ggatggctat gaggtgtata tctgtagcga ccagcctaaa cagcttgtcc gtattgaaga
     2101 tattgtaaaa agcattgata aagaggccgc ttttgttcca cttgaacaat cggtacacga
     2161 aggctttatt gatgaagact taaaaatagc tgtttttact gaacaccaaa tatttggcag
     2221 gtatcaaaag atcaggtacc gcaacgattt tgctgctaac caaactttta cactgagcga
     2281 aattactgat ctccatccgg gcgacttcat tgtacacatc gaccatggag tgggacgctt
     2341 tggtggaatt gaaaaagtag agataaacgg tcactggcag gaggttatca aactggtgta
     2401 ccgcgacaat gatgtacttt acgtaagtat ccactcgttg caccgcatct caaaatacaa
     2461 aggcaaagat ggcgagccgc caagtgttca taagttgggc agtggtgcct ggcagaaaac
     2521 aaaagcggtt gcaaagagca aggtgaagga tatagccaaa gaccttattg cgttgtatgc
     2581 caaacgaaag gaatcgcctg gttttgctta ttctcccgat tcgtttatgc aggaagaact
     2641 tgaagcttct tttatttatg aggatactcc agatcaggaa aaagcaacca tgcttgttaa
     2701 acaaggtatg gaacgtgatt atccaatgga catgctggtt tgtggggatg ttggttttgg
     2761 aaaaaccgaa attgccattc gtgctgcttt taaggctgtg gccgataata agcaggtggc
     2821 cgtgctggtt cctacaacaa tcctggcgct ccagcatttc aaaacctttt ccgaaaggct
     2881 aaaaggcttt ccctgcaaag tagatttcat cagccgtatg cgtaaaccta aggatattag
     2941 ggagattttg aagaaattaa aagaagggga tattaatatt cttattggta cacataaact
     3001 acttagcaaa gatattgaat ttaaagacct tgggctgctc atcattgatg aagaacagaa
     3061 gtttggggtg gctatgaagg aaaagttgaa gcaacttaag ttaaatattg atacgctgac
     3121 acttacggca actccaatac ctcgtacatt gcagttttcg cttatgggtg cacgtgattt
     3181 ggctattatc aatacgcctc cgcccaaccg tcaccccatt gtcacagaag tacattcgtt
     3241 taacgaaacc attatccgtg aaggcattat gtatgagctt atgcgaggcg gacaggtatt
     3301 ttttctgaat aaccgcattg ccaatttgtc cgatttgcaa atggtcattc gaaaattggt
     3361 gccagaagct cgtaccgtta ttgctcatgg gcaaatggat cctgaaaagc ttgaggaaat
     3421 tatcctcgac tttattgacg ggcaatacga tgtgcttatt gctacggcca tcatcgagtc
     3481 gggtatagat atttccaatg ccaacacgat atttatcaat gacgcgcacc acttcgggtt
     3541 gagcgacttg caccagctac ggggcagggt aggacgcagt aacaaaaaag ctttctgcta
     3601 cttgcttact cctccggtta ctacgcttac ttctgaagcc cgccgcaggc ttaaggccat
     3661 tgaagacttt tcagaactgg gaagtggctt tagtatagcc atgcaagacc ttgatatccg
     3721 aggcgccgga aaccttcttg gagctgaaca aagcggtttt attgccgaaa ttggctacga
     3781 aacgtatcaa cgtattctca atgaggctat ccaggaactt aaagaatctg acttcaaaga
     3841 tttgttctat aacgaaaaaa ctgaaacgca aatagctcag ccttcaagca atgtattgaa
     3901 taatgtaatg tatattacag attgtcaaat tgatacagat ttagaaattc tcattcccga
     3961 ggtttatgtg gagagtatca ccgagcgtat caagctttat cgtgaactgg acaaccttca
     4021 gacggatgag gatttggcag catttgtttc gaggcttgct gaccgttttg gacctgtgcc
     4081 acagtcctgt actgatctta tagatgtggt acgcttgcgt cgtatggctt tgcagttagg
     4141 atttgaacgc attgtcctga aaaacaaaca aatggtaatt tattttattg ccaatcaaaa
     4201 atcgccatac tatcaatcac ctgtttttat tggagtactg aatgctgtta ataaaaaccc
     4261 taaggtattc cgcatgaaag aaagcaagga taagctcatt atgtcggtgg acaatatttc
     4321 aaaggtaggc caggcaatag acgtgataag gaaactaaat atggattagc tctattaata
     4381 tttaaatttc ttatctttgt attgttttct ataaatatca aaatttgagg tttatgacaa
     4441 aaaagcagga aaaagaatta gtagcctctt ttaaagaata ttcaaaagag attttgtctt
     4501 caaaagaaaa ttcgcaaaaa tttttgattg aattaggtat ttgtactaaa aatggaaact
     4561 taacccataa ttatcgataa tgtattctaa acgtcacaat ttaattattg gttttcatgg
     4621 ctgtgacgaa actgtaaggg ataagatagt aagaggggag gataacctta agaaaagcac
     4681 aatgattatg attggttagg tcatggcatg tatttttggg aatacaacta tgaaagagct
     4741 cttgattatg ccaaacttat acataaaaat cctgtaagaa gcaaaaataa gatagaaaag
     4801 ccttctgtac ttggggctgt aattgatttg gggcattgtt tagatttgct cgactctgcg
     4861 taccttaaca ttgttaaaga agcgtataag ttacttgaaa aatcttcaaa aaatgcagga
     4921 tttgagttgc ctaaaaatct ttcgatagaa aacgaaaaag atattttaat tcgcaaactt
     4981 gactgtgctg tcattgaggc tgttcatcat tatcacagag aaaaaaatat agttttcgat
     5041 tctgtacgaa atgtattttt tgaaggtgat tgcctttatg aaaatgctgg atttagagag
     5101 aagaaccata ttcaaatttg cgttagaaac ccaaattgta tcaaaggttt ttttattccc
     5161 agaaaccaca ttgagaactg ggatgaataa acttcacaaa gtttatggca acgttcgaag
     5221 agtttggaag tacctaaaac acgttattgg taaaagttta tatgatgaga attttatttt
     5281 tcataggtat aatttttccc tgtacaatgt tgacttccca ggagttacca atatctcatg
     5341 tcattgaatc gtcctccatt tcaactatag aggaggaaat gttctgcaac gacagttcta
     5401 ttgttctcac taatgacagc atggctgtaa tgtgttccaa tctgaatgct aataaaaata
     5461 gctcttttgc aacagtattg attaaagctg gctatggttt gtttctggca gggggaattg
     5521 tattgtgtgc tggatttata ggaggatgga tgatttctgg ccttgttatt ttgacgcaaa
     5581 tgccctattt agcttatgaa attattgcca ttttttcggg ctatattttg tatcggatag
     5641 ggaaaaggat tgagctaaac tttataataa accacaatag aggaaaggcc ttaaacgtta
     5701 atcttcaaaa atattagtga aagacatatt tcaaaggttt taaaaaatct ttcaatagtt
     5761 attttaagaa aaaagagttt actttgccgc aattaaacca acttattaaa tgagattttc
     5821 tgacgatatc ctgcacattg caccatcgcc aataaagctt gccgaagagc tggctggttg
     5881 gttttatgcc caggtaactg cgcatctcga gcaaaatccg aatatataca tcgcattctc
     5941 cggaggaaac agccctatca tgtttttcag gaacattgcc gaaaaatatg gtaaaaagat
     6001 tgaatggcaa agggtgcata tcttttgggc cgacgaaaag tgcgtggccc ctgaccatta
     6061 cgatagtaac tattactgtg ccaataaaca tttgcttagc cggatagaaa tcccggaagc
     6121 caacattcac cgcatacacg gagaaaatac cgataacgag cccaaacgct atgcgcacga
     6181 gattttgagc attgttccaa aataccagga aattccttgc tttgaccttt tcttccttgg
     6241 gatgggcgat gacgggcata ttgcttctat ctttgccggg cagaataatg ttattttttc
     6301 gggcaatatc gttgaaccat cagtagatcc gcgcaaagac caaaagcgta tcaccattac
     6361 gccttattgt atcaacaatt caaaaaaaat catcttcaat gtgtctggat tggctaaatc
     6421 tgccattgtt gatgcagtta taaacaaaaa ggcagaccag ttggagctcc cgggcttaca
     6481 aatcaggcct gctttgggcg aaatgtactg gttttttgat atcggggctg cgcaaaaact
     6541 ttttgtttaa gccttgaaaa cctccattgg aaatatcatt ttagatattg aaaatcaatg
     6601 gaagcaagaa ctttatggcc attgtgctgc aattttcaaa acctgccaca ttccttctca
     6661 tgaccaaacg caccacgcca gggtttggaa ttatgccagt agtttagctg tttctttata
     6721 tttcagggga tatcctgttg ataaaccttt tttgactaag cttatcattt gttgcttttt
     6781 tcacgatacc ggtttatctg tcactttaga tgcttcgcat ggtaaagcca gcgccaatat
     6841 ttgtaaagag tattttaaaa acattgccat taatggtttt gaagaaattt taagggccat
     6901 agaatttcat gaaaataaag atgataaaaa gctttcgtta aagaaaaacc tgctgggcag
     6961 cctcctgact atcgcagacg atttagatgc ttttggatat actggtatat acaggtatgc
     7021 tgaggtttat tctctccggg gtattgatat tgctgaaata tcgtcgtcgg tattaatcaa
     7081 tgccaaagcc cgttatcagc ggattgttaa aagccttccg gcagatgtcc tgaaaacaga
     7141 tgttttatac aataattatc gcgaattggg ccgttttttt gatgtttcta attcggataa
     7201 cgtaacaact cttgtcaaca ctataaaaag caatattata gccaataaac aacaattaaa
     7261 ctctattgtt gaaagtcaac aagctggttt atttaaggat ttgctggtta aaattgaaga
     7321 agaacttaat aaaaacctcg atagctctaa tattgtatgt tttagtaaga atgaaatcac
     7381 tgccagtgat ggaaaaatca atagttatag tttggcttct gcgcttattc tggtgctgtt
     7441 tgttgtttgc ccgttctcac tatggtatga ggatgtttcc ttaaaagaat cgttatttaa
     7501 tgtggcaaaa acggtttata tctggtttat tccattggtt attgtgcatg aagggctgca
     7561 tgccttgttt ttaattctga ccaacaaatt caatgttaaa gccctgaaat ttggctttag
     7621 taaaacttcg ctatcgcctt atacgcatta taatcaagtt gttacaaaag tttcttactt
     7681 agtttcgctt tttgccccct ttgtcctgat aggcataata cctttgttta tatcagcatt
     7741 tacagggtct ggatattggt tcttactttc aataataagt atatggacat gtacaggcga
     7801 tctattggct gtatttcttt tgtcaaaagt accaaatcaa tattttgttc tggatcatac
     7861 agaaagattg gggttttttg ttattgaacg ctaaacaacc ctgaaatcca tcagaagttg
     7921 atcctctaag tacgctttaa gcctgtctcc gggatgtacg cagcccacgc ctggcggggt
     7981 cccagtgtat attagatctc cagtgcgaag ggtcatatat tgcgaaatat gagagaggat
     8041 gatatcgaac ttaaacagca tgtctgatga atacccgttt tgaacaactt ttccatttat
     8101 ttcgagccaa aaatgtatat tttgagggta gggtaatgca gatatttcaa taaattgaga
     8161 acttatagcg gcagaataat caaacccttt ggctatttcc caaggcaagc ctttagcttt
     8221 acattctctt tgtatatcgc gggctgtgaa atcaatgcct aatccaactt cagaataata
     8281 acggcttgca aactgaggtt ttatgctttt gcctaatttc ttgattttca aaaccagttc
     8341 aagttcataa tgtacttcat tcgaaaatga aggaatagga aatggttcgt tatttcgcaa
     8401 aagagcggtg tccggcttta aaaaaaatac tggtttttcg ggtatctcat gttgtatttc
     8461 ttgtgcatgc tgcgcaaaat tgttggctaa acaaaagatt ttcataaatg attgtcttta
     8521 agcagtattt gcatgatatt ttcagaataa gtttttgata ccgggatttc aactaattcg
     8581 ggggtgtcta gctcaatttt taggccatct ttttcttttc gcaacacttt taccctttgt
     8641 gaattgacca taaacgaacg atggcatcgg acgatgtttt gcatttttag ctctggttct
     8701 atctttttca aattatttct caataaaaac ctcgatactt tttgtttgct gttaatgtaa
     8761 taaaggttca cataattgtc tgaagcttca atgtaaagca agttctcgat tttgatggat
     8821 attctcaata cccctttttc atcataaaaa ggaagcatcc cttgaggctg gactgcttgt
     8881 tcccttttaa aaaaattatt ggtcatggca tcaatctgct cttttttgtt ctgataagaa
     8941 aagtatagcc acattaccga atagggcaaa agaataataa gcgcggtagt ctgcagcgat
     9001 ttttttatca attcaaataa aatacgccca tctttgagcc caaattttat aaacaaagca
     9061 taaaacatgg ccatgacaaa tatttcgaca aatatccatg tgccatattg ccaaaaaaga
     9121 agggaatgct ttttataata gtaaaacata attagcctgc tgacaacaac aacaaacact
     9181 cctgtgagaa taaccaggct cgacatgagc agtaacttgt attcagaagc ctccaaccaa
     9241 tatttggccc caaatgggtc ataaatattg ataaatacca aggcaaacaa ggctgtgaaa
     9301 cccacaagcc ttgaaatatt cttacgcgag gtaagatatg atggtattgg atgttctata
     9361 tcaaacattt atagttaggt ttgttgtttg gcaaatgtat aaaaatttat cccttattaa
     9421 aattttttac ccctttatta aaaattttac cccaaaagtc cataaaatgt ctcatttatt
     9481 tgtaatggat atttggattt tattcatttg cacaaaattt caactaaaac atagtaatga
     9541 aaacacctat tgatactgct attgttgcta aagctattga cgaaagcaaa gtgcataatg
     9601 tacaacgggc ctcaatccgc gaggtggttc gtttggtaaa tactattgaa aagcttagcg
     9661 ggactaagtt cgttcgcatg gaaatgggtg ttccgggcat taatgctgaa aaagttggtg
     9721 ttgaagctga aatagagtcc ttaaaaagag gtgttgcttc tatttatcca gatattgaag
     9781 gtattaaaga attaaaagat gaagcttcgc gctttattaa actttttctc gatatcaata
     9841 tttctccttc gggcattgtt cctacggttg gttcaatgca gggtggtttt tgtgcttttc
     9901 ttatgtgtgc tcgtcgcgat gtgaataaaa ctactacctt atttatagac cccggctttc
     9961 cggtacaaaa acaacagcac aaggtactgg gtattccggt cgaacagttt gacgtttata
    10021 attatagagg cgaaaagctg gccgagaagc ttgaatcgta tttgaaaact ggtaaaatag
    10081 cttctattgt ttattcaaac cctaacaacc cttcatggat ttgctttaca gatgatgagt
    10141 tgaagactat tggcacattg gctaaaaaat atgatgttat tgttattgaa gaccttgctt
    10201 attttggcat ggacttccgc caggattatt ctgtgccggg acaggcacca tttcagccta
    10261 ctgtggcaaa atacacagat aactatatgt tgatgatttc aagttccaaa atattcagct
    10321 atgccggaca acgcattggt attttagcca tttctgatac cttatatcat cgtacttttc
    10381 ctgatttgaa aaggtatttc acttcagata gtttcgggtt tgcggctatt tatggggcgt
    10441 tgtatgcatt atcgtccggt acatgtcatt ctgcacagtt tgcattggcg gccatgttca
    10501 aagctgttaa tgatggggcg gctaatctta ctgcttcggt gaaagaatat gaaaaaaggg
    10561 catctctgat gaaagagctc tttgttaaat atggttttaa tattgtttac gataccgacc
    10621 ttggccgtcc attggccaat ggtttttatt ttaccatttc ttatcccgga tatacaggtg
    10681 gtgagctggt agaagaattg ctgccttatg ggatcagtgc gattacttta gatataacag
    10741 gtagtgaaag aaaagaaggc cttcgtgctt gtgtttcgca ggtgagaatg gaacaaatcc
    10801 ctgtcctcga agaacgtttg aaaatatttt acgaaaatca taaataaaac ttattaaata
    10861 tggcgaaagg tgctattgtt gttgataaag acaaatgcaa aggatgtgaa gtttgtgttg
    10921 gggcttgtcc tcagcatgtt attgctatgc aaaaagatgt aaaccgtaaa ggttatcatt
    10981 atgcttatat gaaaaatcct gatgcctgca cgggttgttc taactgtgcc atagtttgcc
    11041 ctgatggtgt tattactgtg tatagggcta aataaattga aagaataata aataaaataa
    11101 tatgagcgaa attcgtttaa tgaaaggcaa cgaggctatt gccgaggctg ccatcagggc
    11161 gggctgtgat gcttattttg ggtatcctat caccccgcaa agtgaggtga tggaatattt
    11221 gatggccgaa cgccctgaaa aacgtacagg aatgattgta ttacaggccg aaagtgaagt
    11281 ggcagctatc aatatggtgt ttggcgctgc tgctgccggc aaaagggtga tgacttcttc
    11341 ttcaagccct ggtattagtt tgaagctcga aggtatctct tatattgccg gggctgacct
    11401 tccatgcgtt atccttaatg tagcccgtgg aggcccgggt ttgggaacta tccagcctgc
    11461 acagtcggat tattttcagg ctgtaaaagg gggtggccat ggcgattacc acatgttggt
    11521 attggcccct gcttctgttc aggaaatggc cgaccacatg ccgctggctt ttgacctggc
    11581 tttcaaatat cgtaccccgg tcatgatatt gtcggacggg gtgattggtc agacaatgga
    11641 gaaagtgaaa ctttttgacc aaatgccacg ctctacagaa gtccctcaat gggctgtttg
    11701 tggtaatgcc ggaagaaaaa accgcattat aacttcgtta gaccttgacc ctgtgaaaaa
    11761 agaacagcaa aacctagctt tgcaggctaa ataccagcaa atgagggaga atgaagtccg
    11821 ctatgaaaag tatttgtgcg atgatgctga ttatttgatg gtggcttatg gtgccagtgc
    11881 acgtatagct aaaaagtcga ttgaatatgc ccgtgaaaag gggattaaag tggggttgct
    11941 tcgtcctatc accttgtttc cttttccttc agcagttatt gctgaattag ccaaaaaaat
    12001 gaaaggtatt ttgtcggtcg agatgagcgc tggccaaatg gtggaagatg ttttactggc
    12061 aacacgccaa acacctcagg tgcctgtcaa acattttggc cgctatggcg gggttgttca
    12121 cagccctgaa gagattttcg aggctttttt aaaacacttt aatttaggat aatatgaatg
    12181 ttaatgatat aattaagccg gaaaacctgg tatattctaa aacgtccctg atgaacgaca
    12241 gggaattgag ttattgtccg ggttgcggtc atggtaccgc caacaggctt gtccatgagg
    12301 tgattgaaga aatgggcatt gggggtgaaa ccattggtgt tgcccctgta ggatgtgcag
    12361 ttatgttata tgaattttct aacctcgatt ttgtccaggc tgcacacggc agggcaacag
    12421 cagtagctac cgggcttaag cgtgtgatgc ctgataaatt tgtattttct taccagggtg
    12481 atggcgacct tgctgctatt gggaccggcg aaacgctgca tacttgtaac aggggcgaaa
    12541 atattactat tatctttatc aataatggta tttatgggat gactggcggt cagatggccc
    12601 ccacaaccct tgtcgggatg aaaacttcaa cttcgcctta tggccgtgat gaaaaatgga
    12661 tgggtagccc tattgatatg accaagctgg tgtctatgct tccggggacg tactatgtta
    12721 cccgccaggc tgtccatacc ccttcggctg tgcgtaaggc gaaaaaggct atccagaagg
    12781 cttttgaggt acaaaaaaat aagctgggac tatcttttgt ggagattgtt tcaaactgta
    12841 actcgggttg gaaaatgtca cctgtgaaag ccaatcaatg gatggaggaa aatatgctcc
    12901 cgtattatcc actgggagac ctaaaggtag atggaaagtt aattactaag taaattacta
    12961 attaccaatg acaaattact aataagaacg agctatttac ttttaataga aaatagtttt
    13021 gatattagta agatgagaaa aaaaaagaaa ctatgacaga agaaataata atagccggat
    13081 ttgggggcca gggagttctt tcgatgggca aaatattggc ttactcgggt atcatgcaag
    13141 atatggaggt gagctggatg ccttcttatg gccctgaaat gcgtggtgga actgctaatg
    13201 taacagttat cctcagcgac aaggagatta gctcgcctgt cctgacccgc tttgatactg
    13261 ccattatcct taaccagcag tcgcttgata agtttgagca atcggttaaa cccggaggca
    13321 ttttattgta cgacaacaat ggtatttcag taaaaccaag tcgtactgat attaaaattc
    13381 atgttgtgga agctacagct gaggctgcac gtatgggctc tcccaaggtg tttaatatga
    13441 tggtacttgg ggcatacctt aagctcaggc ctattgttac tgttgacaat gttattgccg
    13501 gcctgaagaa aagccttcct gcccgccatc atcacttgct cgatgaaaac aaagctgcat
    13561 tgatacgcgg tatggaaatc gttgcttagt tttcaacaca gagacacgga gaacacagag
    13621 gttatactat aaaaaaaacg cgaagctcta tttgaacttc gcgttttttc tttattcttg
    13681 gcccttggtt cttaaatcta aaaaatattc ctctgtgttt tccgtgcccc cgtgttgaaa
    13741 actcactcta cttccttcat tgacaactgg atacgattcc ttactttatc tatttctata
    13801 acttttaccc gtacatgttg gtgtagcttt actatctcgt ttgggtcaga cacaaagcgg
    13861 ttggccaagt gcgagatatg cacaagcccg tcctggtgta cccctacatc cacaaagcac
    13921 ccgaaacggg taatgttggt cacaataccc ggcaatacca tgccttcttt caggtcgttg
    13981 acggtttgta cgttagggtc aaactcaaat actttgataa tggtacgtgg gtcacgtccg
    14041 ggcttggata gttcctgcat gatgtcctga agggtaggca aaccggtttt agccgttaca
    14101 taacgcttga tatctatctt tttttgcaac tccacgtctt tgataagttg ctctacattc
    14161 gtcccaacat cggctgccat tttttcaact attggataac tttcggggtg tactgcactc
    14221 gaatccaaag ggttatgagc gttacggata cgcaaaaaac cagcacattg ctcaaaggct
    14281 ttttcgccca aacgtggcac tttcatcagt tgtttgcgcg actcgaacgc cccgttttca
    14341 ttgcgataag ctacaatagc cgcagctaaa gcaggtccaa ggccagatac gtaggagagg
    14401 agctgcttac tggctgtgtt aagctctacg ccgacgctgt ttacacaaag ttctaccacc
    14461 tggtcgagac tttttttgag cttcccctga tctacatcat gttggtactg cccaacgcca
    14521 atagatttag ggtctatctt taccagctcg gccagcgggt ccatcaaccg gcggccaata
    14581 gatacagctc cccgcacggt gacatcgtat tcgggaaatt catcgcgggc cacttgcgag
    14641 gctgaatata ccgaagcccc gctttcgttg acaatataca cttttatatc ccgctcaaaa
    14701 cggacggttt tgataaagtt ttctgtttcg cggctggcag taccgttccc gatagcaata
    14761 gcttctacat tgtaggtatt taccagggca ttgattttgg ctgctgcttt tcccctttcg
    14821 ttttgcggtg gatgagggta gatggtttcg ttgtgcaaca attttccctg gttgtcgagg
    14881 atgaccactt tacatcctgt cctgaacccg gggtcaatgg ctaaggtctg tttttgtccc
    14941 agtggtgggg ctaaaagaag ctgtctgagg ttttcggaaa ataccctgat ggcctcgtca
    15001 tcggcttttt cttttgcttc gccttttatt tcggtctcta ttgaagggcc aagtaagcgc
    15061 ttgtacgagt ctgttatcgc tttttttaca atatcgctgc actcgttgtc agccttgatg
    15121 aatatctcct cgagcatggc aatggccttt tcctgcgcag gctcgatgct gattttcagg
    15181 aaaccttctt cttcgccacg gtgcatggcc ataaaacggt gcgatgggca acgtttgagc
    15241 ggctcgctaa attcgaaata atcgccgtat ttaatggcat ctggctcttt gcctttcacc
    15301 accttcgata taatcagtgc ttcatgcctg aatagcttcc tgagctggtt acgcgccctg
    15361 acattttcgt ttacccattc ggcaataatg tcggttgcgc cggctatagc ttcatcagca
    15421 ttggcaacat ctttgttgat aaacttttcg gcaaagccat aaatatttac ggtttgttgt
    15481 ttcataatgg taccggccaa tggctctaag cctttttctt tggctatggt ggcccgggta
    15541 cgacgtttgg gcttataagg gagataaagg tcttccagtt cgcttaatgt tgaacaggct
    15601 tctatcttgc tgcgcaattc gtcgctcagc ttgccttgtt cttcaataga attgaggatg
    15661 gtttcgcggc ggttgtcaat ttcaatgagc ttttccatca accgctttat ctcggccact
    15721 tgcacttcat ccaggctgcc tgtcatttct ttgcggtaac ggctgataaa agggatagta
    15781 gcgccttgtt cgagcaggct gatggtgttt tcaacgttct cggcattgat gcccatttgc
    15841 gccgatatct tttctacatg cttagcattc atgctttttc gatatttttt attttccagc
    15901 acgaaattag caaaagatgg gcactttcaa aacagaacgg ggatttatta tgaacaatac
    15961 aatgaagcat atcaacaaaa aacgcttagc attttgaaaa agctaagcgt ttttgcttta
    16021 taaaaataaa ttattctttt actatagcaa cattcacagc attagggcct ttagggcctt
    16081 ttatcacttc aaaggtcact atgtcgtttt cggcaatagc tgctgaaagc gatttggcat
    16141 gtacaaatat atcttgccca gaggccgaat cttttataaa cccaaaccct ttggatgtgt
    16201 caaaaaatgt aacaatgcca cttcggatgg tttcgttgac tttaggcgaa ttgtcacggg
    16261 gtaccccgat ttctatctct tctgcgttga ttacggtctt gcgggtaggg tccggtggag
    16321 tagatgtgat gtttccaaac tcatctacgt aagcaatcat atcgtccagg ttattgccat
    16381 cacggctttg tccttttcgt tcctgtcttc gctgttcttt ttctttcctt ttcttttctt
    16441 tctttgcgcg tacttctttt ttgccaaaag attcatttcc tctgcccatt tattttcctt
    16501 tcttattatt atttaatcga atcaaccaat attggaattt ttcagtatat tacttaatat
    16561 cctacaaggg cagattttta caaaggtaca ttaatttaat tcattattaa ataaaacatt
    16621 tttgatgctt ttacattttg ttgtattggc ggcagatgga caatcaactc cgaaggagtg
    16681 actgtatgag ccccaaaaga atatatttga tttaaatagc agaatcacta gtgtcccatt
    16741 aaaatcggga cgttattaaa aattaaacaa atttggct
//
LOCUS       DAOSPF010000067        16481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3547_length_16481_cov_7.227140, whole genome shotgun sequence.
ACCESSION   DAOSPF010000067 DAOSPF010000000
VERSION     DAOSPF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_16481_cov_7.227140"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    46..145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(483..1025)
                     /locus_tag="PK252_11790"
     CDS             complement(483..1025)
                     /locus_tag="PK252_11790"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOT15433.1"
                     /translation="MRIISGKFRGLTFASKDYNARPTTDFARESLFNILNNYFDFDNI
                     TVLDLFAGTGGISFEFASRGTKSIHLVESDNNNYRQIVDDIKKLKIQGILPFRDDVFK
                     HMERNKAEFDVVFADPPYNADFITQLPDLILNSNAVKSQGWFILEHSKVYHFDNHPFF
                     FQERKYGAVHFSIFVKDKKE"
     gene            complement(1016..1837)
                     /locus_tag="PK252_11795"
     CDS             complement(1016..1837)
                     /locus_tag="PK252_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOT15434.1"
                     /translation="MTENFASLESNTLIIGIFSKEVVYTIFNKETTSFSAIESDKYAS
                     DIELTESIKKIINKESVSAASCEQLILVNQTYRSMLVPESLFDKNNLVTFLKFHHNVD
                     ERDYLHYVKIADSEAYSVFSIPSTIEDLLSSKFEKVHYLHHSIPFISSALSIYNKYNT
                     LPEFYLNVTPDFFDILIIRNKKIQLFNSFLYQKYTDAIYFIVNILNLYSYSPNNVKLI
                     ISGNINKEDNFYSGLAKIFNMVEIQKIPPSVKVEPDMQPVEMQKLVHVFNAVLCV"
     gene            complement(1945..3393)
                     /locus_tag="PK252_11800"
     CDS             complement(1945..3393)
                     /locus_tag="PK252_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOT15435.1"
                     /translation="MKYKLLCITSTVLFLFAGCSESTMDDVNKELNDPLDVSVKNILP
                     SVILQSAFETTGTDIAWYASVYVEHSTGTYNQLYNADRRIGQNAATLLTNNWNSLYEV
                     LNSLKIILDKTSASGPESDFYSARGVAQVLTAYNLAVLTDGWGSVPYSEAFLGLKNMK
                     PKFDKQSFLYVKIQELLDSAIVNLAKNSRAIDAISDYIYKGNVLLWTKAAWSLKARYY
                     MRLTNVDPQAAQKALDCVANGFADNSESLIFNSYEATSVGENPWFQFLNDRFYLSVSK
                     TLYDLMDATADPRIAYYFDVVDTSADYSGKYAYVPAESGNAEQSINGIYSISLITEYG
                     QTAPTPMMTYHELKFIEAEAKRRLGIATFDSSLMQAIAANFSFHGVTESPSDFYNQSV
                     KPLLTGTLEGDLNVIITQKYIAFYEHEAIEAYNDYRRTGIPKLNNRINSMAGYGFVNR
                     FPYGVSETTANIENTPKIDVYKDKVWWAGGTE"
     gene            complement(3412..6657)
                     /locus_tag="PK252_11805"
     CDS             complement(3412..6657)
                     /locus_tag="PK252_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT15436.1"
                     /translation="MRKLALLMCGLALWCGTMLSQGVQIKGKVTSAEDGSALPGASVI
                     VKGTNEATVTDVNGEFTVNTPESASSLYVSFIGMKTQEVQIAGQKSIDIVLQPDVVGL
                     DEVVVTALGIKREKKALGYAVQDVSGNEISKASNPNLLTAMSGKVAGLEIRQSSGMPG
                     ASSQVFIRGARAFSGNNAPLYVVDGMPIKSGTDYDQAVYGVSGSAYSDRTLDIDPNDI
                     ASINVLKGQAAAALYGMRASNGVIIITTKTGKGVQGGSGIVTVNASYSIDQISRYPDY
                     QMTYAQGYNGNFTPVSSFSWGPKISNLPDVPTYGGNSQGHPGQFFHAQKNKWVDAKAV
                     NTPKEFFKDGVTKNYGVTIAQPFSNGDYAIGIGATNQEGIIESSYMDRYTAKGAFNYQ
                     INSKWKTGFTGNYSDVSIGKLPSGNSSWLFTVFGCPPSYDLMGTPYHAPDGANYLYRQ
                     VSYRRSTVGENPLWATKNNKYLEATKRFFGNSYIEYNPFTWMNIHYQLGIDMYTTDNE
                     DILQIGSSQTGQVLPTAPEYPSPNNQTYAYIEPTGGNVDDYGINRRVLNSLLNITFTK
                     QLTDDIKGTLIIGNEINQDNSRKWEMIGTDFFVPGWTNINNTKTQIGDEKKYRNRTVG
                     FFGNASVDYKNMLFFNVTGRNDIVSTMPNGNRSFFYPSVSLGFLFTELDALKGSELLP
                     YGKIRASYAEVGQSDEYHEYTFERGAVGSGFLEDGVKFPLGGNSGFKPKRTLYDPDLK
                     PQNTRSYEFGIELKFFNNRLGIDYTYTDQLAKDQIFKVPLAGSTGYDNIMMNAGEMTS
                     KSHEIVLNIGVIRFNDFKWDISTNYTKVDNKCVKLAEGVESINLGGYEDPNIRASAGE
                     TYPAIYGTQFARDAQGHILVDSDPNSDYYGMPMAGDFGKIGNVAPDFIVGLTNTFTYK
                     TITLSALFDWKQGGQMYSGSNYLIDLYGTSKKTEDRTTPFIYDGYKTNADGSITKNDI
                     QRGGENDPLAYQMLYSDALAGIPEAHVYETSFIKLREISLSIGLPKKWTTPIKVQNAS
                     ISLMARNFLLWTTFKNFDPETSQGNGNMQGGMDYMSLPQTKSYGISLNIVF"
     gene            6908..8191
                     /locus_tag="PK252_11810"
     CDS             6908..8191
                     /locus_tag="PK252_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOT15437.1"
                     /translation="MRLSKGQFYFAMTMLGCLFFIFGFVTWLNGTLIPFFKLACQIST
                     FQALWVTFAFYISYFIMAFPSALVLKKTGYKNGISLGLAIMALGSIIFIPAAYERMYL
                     LFLTGLFVQGTGLALLQTAVNPYVTILGPEHSAAKRISVMGICNKIAGILSPIILGSI
                     ILKNIDQIIKQLENVTNDKNVILDQLSERLIFPYITMAIILTLLAFATKFIKLPDIKA
                     AESPFKTKKSIFHFPYLILGVIAMFLYVGAEVIAGDTIILYGQSLGIPLSKAKFFTSL
                     TLSSMILGYILGIMVIPRIISQSRALAYSAISGIAFAIAAILTQGLVSITFIALLGFS
                     NAIMWPAIWPLSIKHLGSYTEKGAAFLIMAILGGAILPPLYGWLSQLPNLNNKQAYII
                     LLPMYAYILFFAVKGHKITSWKKNQCCPVTSNIVD"
     gene            8484..10583
                     /locus_tag="PK252_11815"
     CDS             8484..10583
                     /locus_tag="PK252_11815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT15438.1"
                     /translation="MAVLEKIRTKMGILVSVIIGFSLLAFVLSDFLGGGKSVFSNNQF
                     DIAEIWGKKVTIQEYEAQVEKLQDVYKMNTRQENIDEKVIDQIREQTWQQVLEDNLLA
                     PEYQSIGLAVSSKELLDMVQGAEPHPYIRQIFSDPETGIFNRPAVLQFIKSLETEPDQ
                     QKKNYWLFMENQIVRERLMTKYYNLMKKGLYVTKVQVANEIKDNQRKVSFNFVGVRYN
                     TIADSTIKVKMSDLKNYLKKHEALFQQEASRDIEYITYDIVPSADDFKATKEWIESIY
                     NEFATTEDIKQIVSLNSDIPFNEKYLKQEEMPDTLKSLYSKGKGAVYGPYFANNAFYL
                     ARITDVKNISDSVKASHILIRPAANTQEAVKAAKATADSLLAVLKKGGNFEELASKYS
                     SDGSAQKGGDLGWFKEGQMVKPFNDACFNGKKGDLTVVETQFGYHVIKITDKGKEVKK
                     VQLALIERKVEASQATEQAIYQKAASFAGNNNTSEKFIQALKKDNIVPRTANYMNENM
                     KEVPGIANSRELVRWAFKAKQNDLSGVMEFGDKYVIARLSAVREKGIASVEQIKDQLT
                     MMVRKEKKAEQLIQKIKQQLKSSSNINDLAGKLGVNVENANDVTFASYALPTIGFEPA
                     VIAAATTVPLNKLSDPVNGNNGVYVVEPTSETVAEVSEENVKSRIYSMYQNAVGYEAV
                     NTLKKMAGIKDMRAKFF"
     gene            complement(10776..12164)
                     /gene="mnmE"
                     /locus_tag="PK252_11820"
     CDS             complement(10776..12164)
                     /gene="mnmE"
                     /locus_tag="PK252_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOT15439.1"
                     /translation="MMQQQESTICAIATPPGHGALGIIRISGPEAFGICNKIIRNKDL
                     TQQASHTIHFCKIYDGPEIIDEALLSIFKGPNSYTGEDTIEISCHGSPYILQKIVLLL
                     IKHGAQMAQPGEYTLRAFMNGKLDLTQAEAVADLIMASSKAAHDTAIDQMKGGFSKKL
                     AGLRDNLLQFASMIELELDFSEEDVEFANREQLRNQVTEIAALVQGLIKSFEYGNAIK
                     NGIPVAIVGKPNVGKSTLLNALLNEEKAIVSDIPGTTRDAIEDTLNIDGIIFRIIDTA
                     GIRHTQDTVENMGIERTFDNIRKASIVLILVDATDEPEEIEKQIKSTSHEGGKDVILV
                     VNKIDKLEKSILDKKLEALSQHLSYPVIALSAKKNINIERIVDTLKKVINSKAPVDNS
                     VIITNIRHAEALQNTFEASQRVLEGLASHLTTDLVAQDIREMIYHLGTITGEITTDEI
                     LGNIFKNFCIGK"
     gene            complement(12224..12955)
                     /locus_tag="PK252_11825"
     CDS             complement(12224..12955)
                     /locus_tag="PK252_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT15440.1"
                     /translation="MNNFYSSLKKISTLFLKFGQYTLTFSGAFFVLIIILAFTPLPYY
                     MQNWLANTDKYDISQPDYIIMLGGSGMPSEDNLIRLYYTAQLGIENPKAYIIIAHPKD
                     SSVQHLMQQELIIRDIDSLRITFASKGTNTQSQIVNIQDSFPETLFKKGIVITSPEQI
                     RRTLMAFRKAGFKHVGGLPSFSQAMDRDFDLRFNSRKLKGSRYIPEVGDKLSLRYNFW
                     NYLKLEITCLRELTALAYYKLNGWI"
     gene            13136..14233
                     /locus_tag="PK252_11830"
     CDS             13136..14233
                     /locus_tag="PK252_11830"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT15441.1"
                     /translation="MNIQNVYQLFNNLKQDNLSFVYHGNFNDEITERIIDISETTIAS
                     RHEEHKISNKVSFLLAECFQNVIRHGGATQEQASLFSSGIFVTRNIGKSYYISSANLI
                     ANEEVEKLREKIEHLNNMSPEELKRLYLDVLEYNTITNKGGAGLGLIEMARKSKQKLD
                     YDFEQVDSHYSYFYLQVKLNSPSEEDTGDKLQENVAKEFHQMLTMDNIFIIHKGDFSQ
                     HTIIPILRMIEENLYKNQERNSYRKRVFHVLVEILQNVSKHGVETNGVKEGIFMMGQK
                     DGHFLIYTGNYIFTSKTAPLAEHLDNLNSMEKPELVELYKLNLHLGATNAQGDAGLGL
                     IDIAREGYSKFQYEFIPVSDSLSFFSLGIFV"
     gene            14240..16297
                     /locus_tag="PK252_11835"
     CDS             14240..16297
                     /locus_tag="PK252_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT15442.1"
                     /translation="MLHPKFFNPSSIVVVGGSNDVKKPGGKLVSNLIAGKYAGVLYIV
                     NPKYDVVQGVQSYANVQQLPDVDLAVLAIPAKDCPATLEFLASQRNTKAFIIISAGFS
                     EESQQGAQYEKAIKETVDKYGASLIGPNCIGVITTQYSAVFTSPVPQLEPDGCDLISS
                     SGATAMFIIESAMSKGLTFSGVYSVGNSTQIGVEEMMEHFDLTFDSEKSSKVKLLYLE
                     SVKNPDKLLFHARSLIKKGCKIAAIKAGSSVAGSRAAMSHTGAMATSDLAVEALFRKA
                     GIVRCFGREELTTVASVFMHKTVKGKRIAIITHAGGPAVMLTDALSVGGLEIPQFDEA
                     KSNELLQHLPHGASAKNPIDILATGTPQQLEIAIDYCEKYFDNIDAIMVIFGSTGLTR
                     VYEAYDVLHRKMQTCCKPIFPILPSVHSAYKELDCFLSRGHINFPDEVQLGHAIAKIF
                     NTAVPAYDAEPEKHVDKAKLRNIIDTAPNGFVSNATIEKILQSANINMVETLEVNNND
                     ALFDGARKIGFPLVVKACGPLHKSDVGGVTLNIKSEAHLLAEFNRMMKIKDTYAVSLQ
                     PMLSGLELFVGAHYEPKFGHIVMCGLGGIFVEILEDVSSGLAPLSYEEAISMVHSLKS
                     YKIIKGARGSKPVSEHAFAQIIVNLSSLLRFAAEIKEIDLNPVIATPYSLTVVDARLR
                     IEK"
BASE COUNT         5023 a         3001 c         3017 g         5340 t
ORIGIN      
        1 acccgcgacc ccatgcgtga caggcatgta ttctaaccaa ctgaannnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnctacc gaaccattta tattttataa gaacttttct
      181 ttttaagtta gttttgaact cgaacccgtc ttgcctcagg caagatgaca ggcatgtatt
      241 ctaaccaact gaactaccga accatttata ttttataaga acttttcttt ttaagttagt
      301 tttgaacttg aacccgtctt gcctcaggca ggatgacagg catgtattct aaccaactga
      361 actaccgaac catttgttct tgcacctaag accttcgttt taggtggtgc aaaagtatat
      421 tcttttttta tttctgcaaa atttcggcct tctttttttt gatttttttt gcagaaaaaa
      481 ttctactctt ttttgtcttt cacaaaaatg gaaaaatgta ctgctccata tttcctttcc
      541 tgaaaaaaaa acgggtgatt atcaaagtga tacactttag aatgttccaa aataaaccat
      601 ccctggcttt taacagcatt tgaatttaaa atcaaatctg gcaattgtgt tataaaatcg
      661 gcattatatg gaggatctgc aaatactaca tcaaactcag ctttattgcg ctccatatgt
      721 ttgaatacat catccctgaa aggcaaaatg ccttgtatct tcaatttttt aatatcgtca
      781 acgatttgac ggtaattgtt attgtccgac tctaccaaat gaattgactt tgtccccctt
      841 gatgcaaatt caaaacttat gcctccagtg cccgcaaaca aatcaagtac tgtgatattg
      901 tcgaaatcaa agtaattatt caaaatattg aataggcttt cgcgggcaaa gtcagttgtg
      961 gggcgtgcat tataatcttt tgaagcaaat gtaaggcccc gaaatttccc ggatattata
     1021 cgcataaaac agcattaaaa acatgaacta acttttgcat ttctacaggc tgcatatctg
     1081 gctcaacttt tacagatggc ggtattttct gtatctcaac catattgaat attttggcta
     1141 atcctgaata aaaattatct tctttattaa tatttcctga tatgataagt tttacattgt
     1201 taggcgaata agagtaaagg ttcaatatgt ttactataaa atatattgca tccgtatatt
     1261 tttgataaag aaatgaatta aacaattgta ttttcttgtt ccttattatt aatatatcaa
     1321 aaaaatcggg tgtgacattg agatagaact caggtaacgt attgtatttg ttataaatac
     1381 tcaaagcgct tgatatgaaa ggaattgagt ggtgcagata atgtactttc tcaaattttg
     1441 aagataacaa atcttcaatc gtcgaaggaa tagagaacac tgaatatgct tccgaatctg
     1501 caattttcac gtaatgcaga tagtctcttt catctacatt gtgatgaaat ttcaaaaagg
     1561 ttaccaggtt gtttttgtca aataagcttt cgggtaccaa cattgacctg taggtctggt
     1621 ttaccaggat aagttgttca caactggccg cagatacaga ttctttattt attatttttt
     1681 ttatactttc tgtaagttca atatctgaag catacttatc agactcaatg gcagaaaaag
     1741 aggtagtttc tttattaaaa atggtataaa ctacttcttt tgagaatatc cctataatca
     1801 gtgtattact ttctaatgaa gcaaaattct ctgtcatatt tctatttttc tcaaagttag
     1861 aaaaaaacaa gcaaaacata aaaaaaggta ctctttattt caggagtacc tcttttatct
     1921 attcattttg tatagtctct gttattattc agtacctcca gcccaccata ctttatcttt
     1981 atatacgtca atcttaggcg tattttctat attagctgta gtttcggaaa ctccataagg
     2041 aaaacggttt acaaaaccat aaccagccat agagttaatc ctattattta atttagggat
     2101 acctgtcctc ctgtaatcat tataagcttc aatagcttca tgttcataaa aagctatata
     2161 tttttgtgta ataataacat ttaaatcacc ttctaatgtt ccggttaata atggttttac
     2221 cgattggttg taaaaatcac tcggactttc tgtcactcca tggaaactaa agttggcagc
     2281 aatagcctgc attaatgaag agtcaaatgt ggcaattcct aaacgccttt ttgcttctgc
     2341 ttcaataaac tttagctcgt gatatgtcat catcggtgtt ggtgctgttt gtccatattc
     2401 tgtaattaat gatattgaat aaattccatt tatagattgt tctgcattgc cactttctgc
     2461 tggaacataa gcatacttgc cactataatc agcactagtg tcaactacat caaaatagta
     2521 tgcaatacgt gggtctgcag tggcatccat taaatcatat aatgtttttg atactgacaa
     2581 gtaaaaacga tcatttaaaa attgaaacca agggttttca cctacagatg ttgcttcata
     2641 agaattaaaa attagggact cagaattatc tgcaaaacca tttgctacac aatccaaagc
     2701 cttttgtgca gcttgagggt caacgtttgt aagacgcata taatatcttg cttttaacga
     2761 ccaggcagct tttgtccata acaaaacatt tcctttatat atatagtcag atatggcatc
     2821 aatagccctg gaatttttgg caagatttac aatagcagaa tctaacaatt cctgaatttt
     2881 gacatacaaa aaagattgtt tatcaaattt gggtttcata tttttcaatc caagaaaagc
     2941 ctcgctataa ggaacagatc cccaaccatc tgtcaaaaca gccaaattat atgcagttaa
     3001 aacttgtgca acacctcttg ctgaataaaa atcagattca ggaccagatg cagatgtttt
     3061 atccaaaatt atttttaatg aatttaatac ttcatataaa ctgttccaat tattagttag
     3121 caatgttgct gcattttgtc ctatgcgacg atcggcatta tataattggt tatatgtccc
     3181 ggtactatgc tcaacataaa cagatgcata ccaggcaata tctgtacctg tggtttcaaa
     3241 tgcactctga aggataacag aaggaagaat attttttacg ctaacatcca atggatcgtt
     3301 caattcttta tttacatcat ccatggtgct ttcactacag ccagcaaata aaaatagtac
     3361 tgtagaagtt atacatagaa gtttatattt cataacaaat taatttaaag gttaaaaaac
     3421 aatatttagg cttatgccat aagattttgt ctgaggtaat gacatatagt ccattcctcc
     3481 ttgcatgttc ccatttcctt gagatgtttc agggtcaaaa ttcttaaaag ttgtccacaa
     3541 taagaaattt ctggccatta atgaaataga cgcattttga actttaattg gagttgtcca
     3601 ctttttaggt aagccaatgg atagagatat ttcccttaat ttaataaatg aagtttcata
     3661 aacgtgagct tcagggattc cggccagggc atcagagtac aacatttgat atgcgagtgg
     3721 gtcattttcg cctcctcgct gaatatcatt ctttgtaata ctaccatcag cattagtttt
     3781 atatccgtca taaataaaag gggtagttct atcttctgtt ttcttagaag taccgtatag
     3841 gtctattaaa tagtttgaac cagaatacat ctgaccgcct tgtttccaat caaataatgc
     3901 agacaatgta attgttttat aagtaaaagt attagttaac cctacaataa aatcaggagc
     3961 tacatttcct atctttccaa aatcaccagc cataggcata ccataataat cactattagg
     4021 gtcactatct accaatatat gtccttgagc atctcttgca aattgagtac cataaattgc
     4081 cggatatgtc tcaccggcag aagcacggat gtttgggtct tcatatcctc ccaaattgat
     4141 actttcaact ccttcagcta atttgacaca tttgttatct acttttgtat aatttgttga
     4201 tatatcccat ttaaaatcgt tgaatcggat tacacctata ttaaggacta tctcatgaga
     4261 tttactggtc atttctcctg cattcatcat aatattgtca tagcctgttg atcctgcaag
     4321 aggtacttta aaaatttgat ctttggccaa ctggtccgta taagtataat caatccctaa
     4381 cctattgtta aaaaatttaa gttcaattcc aaattcatac gaccgtgtat tttgtggttt
     4441 taaatctgga tcgtataatg tcctttttgg tttaaaacca ctattgcctc caagcggaaa
     4501 ttttacgcca tcttcaagga atccacttcc aacagccccc ctttcaaaag tatattcgtg
     4561 atattcatca ctttggccca cttctgcata agaggctctt atttttccat aaggcaataa
     4621 ctcacttcct tttaaagcat caagttctgt gaataaaaag cctaatgaaa ctgatggata
     4681 aaagaatgac ctatttccat taggcatagt agaaacaatg tcatttcttc ctgtaacatt
     4741 gaaaaatagc atgtttttat aatctacaga agcattcccg aagaaaccta cggttctatt
     4801 cctatatttt ttttcatcgc caatttgggt ctttgtgttg tttatatttg tccaacctgg
     4861 aacaaaaaaa tcagtaccaa tcatttccca tttacggcta ttatcctggt ttatttcatt
     4921 acctattata agagttcctt taatatcatc tgtaagttgt tttgtaaagg tgatatttaa
     4981 aagtgagttt aacactcttc tgtttatccc ataatcatca acattgcctc ctgttggttc
     5041 tatataagca taagtttgat tatttggtga tggatattcc ggagctgttg gtaaaacttg
     5101 acctgtttga gaactaccta tttgtaggat atcttcattg tctgtagtat acatatctat
     5161 tcccaactga taatgaatgt tcatccaggt aaatggatta tattctatgt aagaatttcc
     5221 aaaaaacctt ttggttgctt caaggtattt attgtttttt gttgcccata atggattctc
     5281 gccaacagta ctacgcctgt aacttacttg tctatataaa taattggctc catctggggc
     5341 atggtaaggt gttcccatta agtcgtacga aggagggcag ccaaatacag taaataacca
     5401 actactattt cctgaaggaa gttttcctat agatacatca gaatagtttc cagtaaaacc
     5461 tgttttccat tttgaattaa tctgataatt aaaagctccc ttagctgtat atctgtccat
     5521 gtatgaactc tcaataatac cttcttggtt agttgctcct atgccaatag catagtctcc
     5581 atttgaaaat ggttgagcta ttgtaactcc ataatttttt gtaaccccgt ctttaaaaaa
     5641 ttcttttgga gtatttacag ccttcgcatc tacccatttg tttttttgag catgaaaaaa
     5701 ttggccagga tggccttgag aattaccacc gtatgttggc acgtctggta aattggaaat
     5761 tttggggccc caggaaaaag aactgacagg ggtgaaattt ccattataac cttgagcata
     5821 tgtcatttga taatctggat atctggaaat ttggtcaata gagtagctgg cattaacagt
     5881 tactattccg gagccaccct gaactccttt tcctgtctta gtagtaataa taattacacc
     5941 gttcgaagct ctcattccat ataaagccgc agcagcctgc cctttcaaaa cgttaattga
     6001 tgctatgtca tttgggtcaa tatctaaggt cctgtcagaa tatgcagacc cactcacccc
     6061 atatactgct tggtcatagt ctgttccact tttaataggc attccgtcaa caacataaag
     6121 tggggcatta tttccactaa aagcacgtgc accacgaatg aaaacttgag atgaagctcc
     6181 aggcatacct gatgattgac gaatttcgag acctgctact tttccgctca ttgctgttaa
     6241 taaatttgga ttgctggctt tgctaatttc attgccagaa acatcttgaa ctgcataacc
     6301 gagggctttt ttttctcttt taatacccaa tgcagttaca actacttcgt ccaaaccaac
     6361 aacgtctggc tgaaggacaa tatcaatact tttctgtcca gctatttgaa cctcttgtgt
     6421 tttcatccca atgaaactaa cataaagaga tgatgcactt tctggtgtat taactgtaaa
     6481 ctctccattt acatctgtca cagtagcctc attagtacct ttaactatga cagatgcccc
     6541 cggcaaggcc gatccatcct ctgccgaggt tactttcccc ttaatctgta ccccttgcga
     6601 tagcattgta ccgcaccaca aggccagacc acacattagt aatgctaatt ttctcataca
     6661 agtgattttt tggtttataa ttggttaata cataaaactc aatctctcaa atatatatat
     6721 ttttcaatat attatcaaat gatagcttat aattattcac tttttgcata gtttttatta
     6781 ataattttta tttattgatt ttcaatgctt tacaagtgtt tgaaaataag ttgttaacag
     6841 tttctggcct aaaacttttg cctgaagctt tttttcgcaa tatttgtgca acaaaataat
     6901 gcattttatg agactttcaa aaggccaatt ttactttgcc atgaccatgc ttggttgcct
     6961 tttttttatt tttggttttg tcacctggct caatggtaca ttgatcccgt tttttaaact
     7021 ggcttgtcaa atcagtactt ttcaggcatt gtgggtgacg tttgcatttt atatttctta
     7081 cttcatcatg gcttttccgt cagctctggt actgaaaaaa accgggtaca aaaatggcat
     7141 cagccttgga cttgccatta tggcattggg aagcattata tttatcccgg ctgcatacga
     7201 acgtatgtac ttgttgtttc tgacaggttt atttgttcag ggaacagggc ttgctttgct
     7261 tcaaacagcg gtaaaccctt atgttacaat attaggccca gaacatagtg ccgcaaaacg
     7321 tattagtgtt atggggatat gtaataaaat tgcgggtatc ctgagcccaa taatcttagg
     7381 ctcaattata ctaaaaaata tagaccagat aatcaaacag cttgaaaatg taacaaatga
     7441 caaaaatgta atcctggatc aattgtctga aagattgata tttccttata taactatggc
     7501 tataatattg acattgcttg cttttgctac aaaatttatc aagttgccag atattaaagc
     7561 cgccgaaagc ccttttaaaa ctaaaaaaag tatctttcat tttccatatc ttattttagg
     7621 cgtaatagcc atgtttttat atgtcggggc agaagtcatc gccggagata ccattatatt
     7681 atatggacag tctttgggca tacctttgtc taaggccaaa ttctttacca gcctgacatt
     7741 aagctccatg attttgggct acatattagg aataatggtt atcccccgga tcatttcaca
     7801 aagcagagca ttggcttatt ctgcaatatc aggaatagct tttgccatcg cagctatttt
     7861 aacgcaaggg cttgttagta ttacatttat cgcattattg gggttttcca atgctattat
     7921 gtggccggct atatggcctt tatctataaa acatttagga agctatacag aaaaaggagc
     7981 tgcttttctg attatggcta ttttaggcgg ggccatcctt cctccgcttt atggttggct
     8041 ttcacaattg ccgaatttga acaataagca agcttatatt atattgttgc caatgtatgc
     8101 ttatattttg ttttttgcgg tcaaagggca caaaattact tcatggaaaa agaatcaatg
     8161 ctgtccggta actagtaata ttgttgatta gaaactggtc gccccgccgg ggcttaattt
     8221 taacgatgtt tattttacta caaacaggac atcgctacgc gactaaataa tagcagcgta
     8281 gctgcgaact gtttgtagga tacccaaata cgccaaatgt tcctaagctg catagcagcg
     8341 acccgttttt accggacaac aatgaaaata atttggaaaa taaatcagca taaataaaaa
     8401 tagcagcttg tagtttttaa gaatattagt atattcgtag cctcaaaaaa atagtgtgta
     8461 actttttata atcaaataat tcaatggctg ttttagagaa aataaggacg aagatgggaa
     8521 ttctggtttc cgtcattatt ggtttttcat tgttggcatt tgtacttagc gactttttag
     8581 gtggaggaaa gtctgtattt agtaacaatc agtttgatat agctgaaatt tggggcaaaa
     8641 aagtcactat tcaagagtac gaagcccagg ttgaaaaact tcaggatgtt tacaaaatga
     8701 atacccgtca ggaaaatatt gacgagaaag taatagacca aatccgcgag caaacctggc
     8761 agcaggttct tgaagataat cttcttgctc ccgaatatca atcaataggg ttagcggttt
     8821 cgtcaaaaga acttttggat atggtccaag gggcagaacc tcatccatat atccgtcaaa
     8881 tatttagcga tcctgagaca gggatattta acaggccagc cgtgttgcag tttatcaaat
     8941 cattagaaac agagccagac caacaaaaga aaaactattg gttgtttatg gagaaccaaa
     9001 ttgttcgaga acggttgatg acaaaatatt acaaccttat gaaaaaaggc ctgtatgtaa
     9061 ctaaagtaca ggttgctaat gaaataaaag ataatcaacg gaaagtttct tttaactttg
     9121 taggtgtcag gtataatact attgctgaca gcactattaa agtaaaaatg tctgatttga
     9181 aaaattacct gaaaaaacat gaagctttat ttcaacaaga agcttcacgt gacattgaat
     9241 atattactta tgacatagta ccttctgctg atgacttcaa ggcaactaaa gaatggattg
     9301 aaagtattta caatgaattt gctacaaccg aagatatcaa acaaattgtt agccttaact
     9361 cagatattcc ttttaatgaa aaatacctga aacaagagga aatgccagac actcttaaaa
     9421 gtttgtacag taaaggtaag ggagctgttt atggcccata ctttgcaaac aatgcatttt
     9481 accttgcacg tattaccgat gttaaaaata tttcggacag tgtaaaggca agccatattt
     9541 tgattcgtcc tgcagccaat acccaggaag ctgttaaggc tgcaaaagct actgccgata
     9601 gcttgttagc tgtgttgaaa aaaggaggga actttgaaga attagcatct aaatactcaa
     9661 gtgatggtag tgctcagaaa ggtggtgatt taggttggtt taaagaaggc cagatggtaa
     9721 aaccttttaa tgatgcctgt tttaatggga aaaaaggaga tttgactgtt gtagaaaccc
     9781 aatttggtta tcatgtaatc aaaattaccg ataaaggaaa agaagttaaa aaagtacaac
     9841 ttgctttgat tgagcgtaaa gttgaagcca gtcaggcaac agaacaagcc atttatcaaa
     9901 aagcagcttc atttgccgga aacaacaata ctagtgaaaa atttatccag gctttgaaaa
     9961 aagataacat cgtcccgcgt acggcaaact atatgaacga aaacatgaag gaagttcctg
    10021 gcattgccaa ctccagggaa ttggtacgtt gggcttttaa agcaaaacaa aacgatctgt
    10081 ctggtgttat ggaatttggt gacaagtatg taatagcccg tctttctgcc gttagagaaa
    10141 aaggtattgc ttctgttgaa caaattaaag atcagttgac catgatggtt cgtaaagaga
    10201 agaaggctga gcagcttatt caaaaaatta aacaacaact gaaaagttct tcaaacatta
    10261 atgaccttgc cggaaaattg ggtgttaatg ttgaaaatgc caacgatgtt acatttgcct
    10321 cttacgcatt gcctacaatt ggttttgaac cagctgtgat tgcagctgca acaaccgttc
    10381 cgttaaacaa actctctgat cctgtcaatg gtaataacgg ggtttatgta gttgaaccta
    10441 catctgaaac tgttgctgaa gttagtgaag aaaatgttaa aagtaggatt tactcaatgt
    10501 accaaaatgc agttggatat gaagctgtta atactttgaa aaagatggca ggcattaaag
    10561 atatgcgtgc aaaattcttt taaaaatgct tgtttgtaag tgtaaaaaaa tattgcttat
    10621 acttctaaga taaaatcata taatgctgtt cggcgaaaat gaaagcggac agcattattt
    10681 ttttgtctaa tctgtgtcct tattagtaga tattattgta gtttgctaat tgtgcttttt
    10741 agtatttttc ggtaataatg ggatacctgg atactttact ttccgataca aaaattctta
    10801 aaaatattcc ctaaaatttc atctgttgta atctcgccgg taatggtacc aaggtggtaa
    10861 atcatttcgc ggatgtcttg cgctacaagg tctgtagtga ggtgagaagc cagtccttcc
    10921 aatactcttt gtgatgcttc aaaagtattt tgtaaagctt ctgcatgtct tatattggta
    10981 atgataacac tattgtcaac cggagctttg ctgtttatta cttttttaag cgtatccaca
    11041 attctttcaa tgtttatgtt tttcttagct gataaagcta taaccggata agataagtgc
    11101 tggcttaaag cttccagctt tttatcaaga atagactttt caagtttatc tatcttattg
    11161 acgacgagga ttacatcctt acctccttca tgtgaggtgg actttatctg cttttcaatt
    11221 tcctcaggtt cgtctgtggc atctaccaat ataagtacaa tgcttgcttt acggatattg
    11281 tcgaatgtac gttctatgcc catattctcg acagtatctt gcgtgtgacg tattccggct
    11341 gtgtcaataa tgcgaaaaat gataccgtct atatttaaag tatcttcaat agcatcacgc
    11401 gtggtaccgg gtatatctga tactattgct ttttcttcat taagcaaagc attgagcagt
    11461 gtggatttgc caacattagg tttaccaaca atagctacag gtatgccatt cttgatggcg
    11521 ttaccgtatt caaaagattt gataaggccc tgaacaagtg cagcaatctc tgttacttgg
    11581 ttacgcaatt gttcacgatt ggcaaactca acgtcttctt ctgaaaagtc tagttctaac
    11641 tcaatcatcg aggcaaattg caataagtta tcccgcaatc cggccaattt tttggagaat
    11701 cctcctttca tctggtctat ggcagtgtca tgggctgctt tggaggaggc cataatcaaa
    11761 tcagctacag cttcggcttg tgtgaggtca agcttgccgt tcatgaaggc gcgtagggta
    11821 tattctcctg gttgggccat ttgggcacca tgtttgataa gtagcaaaac tattttttga
    11881 aggatgtagg gtgagccatg acaactgatt tcaatagtat cttcgccggt atatgagtta
    11941 ggccctttga aaatgctcag cagcgcctcg tcaataatct ctgggccatc gtatattttg
    12001 caaaaatgaa tggtatgaga ggcttgttgt gttaagtctt tgtttcggat gattttgtta
    12061 caaatgccaa atgcctcggg cccgctaata cggataattc ccaatgcccc gtgtccggga
    12121 ggggttgcta tggcacatat tgttgattct tgttgttgca tcatctcaaa tttatgcaaa
    12181 gataagggtt tttactttta gtggtgataa atcctgtttg gggttaaatc cagccattta
    12241 gcttgtaata tgccaaagct gttaattctc tgaggcaggt aatctccagt ttcagatagt
    12301 tccaaaaatt ataacgcagg ctaagtttat ccccaacttc agggatatac ctcgagcctt
    12361 tgagttttcg gctattgaat cgcaggtcaa aatctctatc catggcttgt gaaaaagacg
    12421 gcagccctcc aacatgctta aaccctgctt ttctgaaagc cataagtgtg cgccgtattt
    12481 gttcgggact ggtgatgact attccttttt tgaataaagt ttcgggaaaa ctatcttgga
    12541 tattcactat ctgagattgc gtattggtac cttttgaagc gaacgtaatt cgcagcgaat
    12601 caatatctct tattatcagt tcttgttgca tcaaatgttg cactgagcta tctttgggat
    12661 gggcaataat aatatatgct ttagggttct caatccctaa ttgagcagtg taatatagtc
    12721 ttatcaggtt gtcctcgctt ggcattccac tacctccaag cataataata taatcaggct
    12781 gcgaaatgtc atatttgtct gtatttgcaa gccaattttg catatagtag ggcaaaggtg
    12841 tgaaagccag aattattatc aagacgaaaa atgctccaga aaaggtaagt gtatattgcc
    12901 caaacttcag aaataatgtg gatatttttt ttaaagatga atagaaatta ttcatattat
    12961 tttaaagtgt tgtaatgtgt tgatattaaa atatttatta cttaaatata ctaagaataa
    13021 ctatgtttaa aggtacacaa atatcatcat tcataaacat ttacatgaaa ggggcttttt
    13081 tgcaataaaa aaatatttat atttatccac aaaaattaca actacccaac tactcatgaa
    13141 tatacaaaat gtatatcagt tgtttaataa cctgaaacag gataatctta gctttgtata
    13201 tcatggcaat ttcaacgatg aaatcaccga acgtattatt gatatcagcg aaactaccat
    13261 agcttcgagg catgaggaac ataaaattag caataaggtt tcgtttttgc ttgccgaatg
    13321 ctttcaaaac gttatcagac atgggggggc cacgcaagaa caagcatctc tttttagtag
    13381 tggtattttt gtaacccgta acatcgggaa atcatattat atctcatctg ccaatcttat
    13441 tgctaatgaa gaggtagaaa aactgcgcga aaagattgaa cacctaaaca acatgtcgcc
    13501 agaagagctt aaaaggctgt atttagatgt tttagaatac aatactatta ccaataaggg
    13561 gggggcaggc cttggactta ttgaaatggc ccgaaaatca aagcaaaagc ttgattatga
    13621 ttttgaacag gtcgattcgc attattctta cttttatttg caggtaaagc tcaattcgcc
    13681 ttcagaagaa gatactggag ataaactgca ggaaaatgtt gccaaagagt ttcaccagat
    13741 gttgacaatg gacaatatat ttatcattca taagggggac ttttcgcaac ataccataat
    13801 tcctattctt cggatgatag aagagaatct ttacaaaaac caggaacgca attcgtatcg
    13861 aaaaagagtg tttcacgtgc tggtagagat attgcaaaat gtgagcaagc atggcgttga
    13921 aaccaacggg gtgaaagaag gcatattcat gatgggccag aaagatggcc attttcttat
    13981 ttataccgga aattatattt ttaccagtaa gacagcgcct cttgctgagc atctggacaa
    14041 tctcaattcg atggaaaaac ctgagctggt agaattgtat aaacttaatt tgcaccttgg
    14101 agctaccaat gcacagggcg atgccgggct gggccttata gacattgccc gcgaagggta
    14161 tagcaagttt cagtacgaat ttattccggt ctcagatagc cttagcttct ttagtttagg
    14221 tatctttgtt taaaaattca tgttacatcc caaattcttc aatccttcat ccattgtggt
    14281 ggttggaggt agcaatgatg ttaaaaaacc tgggggcaag ctggtaagta accttattgc
    14341 aggcaaatat gctggagtgt tatatattgt caatcccaaa tatgatgtgg tacagggagt
    14401 tcagagttat gcaaatgtcc aacagttgcc cgatgttgat ttagctgtat tggctattcc
    14461 ggccaaagac tgtcctgcta ctcttgaatt tcttgcttca cagcgcaata caaaagcttt
    14521 tattattatt tcagcgggtt ttagcgaaga aagccaacaa ggagcacaat atgagaaagc
    14581 tattaaagaa actgttgaca aatacggggc ttcgcttatt ggccccaatt gtatcggtgt
    14641 gattacgacg caatacagtg ctgtattcac ttctcctgtg ccacaattgg aacctgatgg
    14701 ctgcgacctc atttcaagtt ccggggctac ggctatgttt attattgaat cggcaatgtc
    14761 caaaggatta accttttcag gggtttattc tgttgggaac agcacgcaga ttggtgtaga
    14821 agaaatgatg gaacattttg acctgacttt cgactccgaa aaatcgtcaa aggttaagtt
    14881 gttatatctc gaatctgtta aaaatcctga caaactgctg tttcatgccc gctcgctgat
    14941 aaagaaaggc tgtaaaatag cagctataaa agctggcagt agcgtggccg ggagcagggc
    15001 agctatgtcg cataccgggg caatggccac ttccgatctg gctgttgaag ctttgtttag
    15061 aaaagccggc attgtcaggt gttttggacg cgaagagctt actactgttg cttcagtttt
    15121 tatgcataaa actgtaaaag gtaagcgtat tgccattatc actcatgccg gaggccctgc
    15181 tgtaatgctt accgatgctt tgtctgtcgg aggccttgaa atacctcaat ttgatgaagc
    15241 taaaagcaat gagctattac aacaccttcc tcatggggct tctgccaaaa atcctattga
    15301 cattttggct accgggactc cccaacagct tgaaattgct attgattact gcgagaaata
    15361 ttttgataat attgacgcca tcatggttat ctttggcagt acaggcctta cccgtgtgta
    15421 tgaagcctat gatgtgctgc accgaaaaat gcagacgtgt tgcaagccta ttttccctat
    15481 attgccatcg gtacattcgg catataaaga gctggactgc tttttatcgc gagggcatat
    15541 caacttcccc gatgaagtgc agcttggaca tgccattgcc aaaatattca atactgctgt
    15601 gcctgcttat gatgccgagc cagaaaagca tgttgacaag gcaaagctga ggaatattat
    15661 tgatactgcc ccaaatggct ttgtcagcaa tgctactatt gaaaaaatat tgcagagtgc
    15721 caatattaac atggttgaaa ctttagaagt taataacaat gatgctttgt ttgatggcgc
    15781 tcgaaaaatt ggtttcccat tggtggtcaa agcatgcggg ccgcttcata aaagtgatgt
    15841 aggaggggtt acccttaata tcaagtctga agcccatttg ttggcagagt ttaaccgcat
    15901 gatgaagata aaagatacgt atgctgtgag tttgcagcca atgttatcag ggttggaact
    15961 atttgtgggc gctcactacg aacctaagtt tggacatatt gtaatgtgtg ggcttggggg
    16021 aatctttgtt gaaatactgg aagatgtctc ttctggttta gctccattat catacgaaga
    16081 agccatctcc atggtgcatt cgctgaaaag ttataagata atcaaagggg cacgcggaag
    16141 caagccagtt tcagaacatg ccttcgccca aatcattgtc aatctctcat ctttattgcg
    16201 ctttgctgca gaaatcaagg aaatagattt gaaccctgtc atagcaacac cttattcgtt
    16261 gactgttgtt gatgcacgct taaggataga aaaataactt ttttaatttg ttccagaatt
    16321 tattttgcat gcattgaaat attcacataa tacccccttt tttatttcaa aatatagtga
    16381 atatggcata attattgatg ttgtcagggc atctcaacaa tagtcctact tgtcaagttt
    16441 cacaaggttg tttatgataa cttaattgca aagataaaac t
//
LOCUS       DAOSPF010000068        16170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_3631_length_16170_cov_7.797580, whole genome shotgun sequence.
ACCESSION   DAOSPF010000068 DAOSPF010000000
VERSION     DAOSPF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3631_length_16170_cov_7.797580"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PK252_11840"
     CDS             complement(<1..617)
                     /locus_tag="PK252_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT15443.1"
                     /translation="MAGKKMITAEQARVIVEKSLVNVARGRKPVELYEPAKYILGLGG
                     KRIRPVITLLSCNLFSENIKNAINPAVGLEVFHNYTLVHDDIMDNALLRRGKDTIHAL
                     WGNNTAILSGDAMSILAYELMLQSPVSVLPSVLKTFTQTALEVCEGQQYDMNFETLDS
                     VSEQDYLEMIKLKTSVLLAGCMKIGALLGGASEAEAERLYQVGLNA"
     gene            758..2026
                     /gene="purD"
                     /locus_tag="PK252_11845"
     CDS             758..2026
                     /gene="purD"
                     /locus_tag="PK252_11845"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT15444.1"
                     /translation="MNVLLIGSGGREHAIASKIIQSKHLDQLFITPGNAGTANIGVNI
                     ELAPYNFESIGKFACANRVDMVVVGPEEPLVNGLADYFLQNERLKNIPVIGPVKAGAM
                     LEGSKDFAKEFMVKYNIPTAKYFTVTQNNINEGFAFLQSMNAPYVLKADGLAAGKGVV
                     ILNNIDEAKAELEAMLSGKFGSASNKVVIEQFLKGIELSVFVLTDGQSYVILPEAKDY
                     KRVGEGDTGLNTGGMGAVSPVPFANTEFMHKVEQRIVIPTIEGLKKENIKYKGFIFFG
                     LIKVDGEPYVIEYNVRMGDPETEVVFPRIKNDVLELFQAVANETLANQKLVIDERVAT
                     TVMLVSGGYPGDYKKGYEINNLGKVQNSIVFHAGTTLNDGIVRTAGGRVIAITSFGKT
                     KEEALLQSYKNAEIIDFKDKYFRKDIGFDL"
     gene            2039..3019
                     /locus_tag="PK252_11850"
     CDS             2039..3019
                     /locus_tag="PK252_11850"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOT15445.1"
                     /translation="MLLRVSRSSNPSLPIILLILSLVFWFNGFTEVSLNNQIYHFSPF
                     YDLIKLLPQQAGIIQSLLGILALWFVAFSLIRINSRFIIIQERTYLPVLFYFVMIFAI
                     TKINYLHPALIAAIFISIALERLFHSNKNENLSYNPFDAAFFITLAAFFYFPAIFFNV
                     IVWLALLIMRPFYWREWVYSIIGMFVPSFIILSIFYLTGTNFQYILKSAKLLFTVKND
                     ISITITDALFLSFCLVMLLIASAHMIAMMGKFKILTRKAYNLFLALFLVSVVVYIFVP
                     SVGLEIIFIGALPISQLFTNYYLNAPATRFRETVFDLFIIFFVLTQLIKQ"
     gene            complement(3009..3425)
                     /gene="tsaE"
                     /locus_tag="PK252_11855"
     CDS             complement(3009..3425)
                     /gene="tsaE"
                     /locus_tag="PK252_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT15446.1"
                     /translation="MESFEISSLEDLHKPAESIISKSAHKPVVAISGKMGAGKTTLIK
                     TICKALNVVGNVTSPTFSVVNEYLTASGKKIFHFDFYRLNKAEELYDLGYEEYFYSGN
                     LCLIEWPEIAENILLPDTLQVNIEVNNKQKRLITIA"
     gene            complement(3554..5107)
                     /locus_tag="PK252_11860"
     CDS             complement(3554..5107)
                     /locus_tag="PK252_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOT15447.1"
                     /translation="MTREITILWADDEIELLKPHILFLENRGYKIISVTNGDDALQIA
                     QKGTVDIVFLDENMPGKSGLELIEPLKRILPGVPVVMITKSEEENLMEDAIGSQIDDY
                     LIKPVNPNQVLLTLKKHIDNKRLVSSKTTQTYQTQFMQISQRINSAQSIEDWKEIYKN
                     LVFWELELEKTGQGLSEVLKNQKSEANHEFGRFIKSSYAKWFKENAQDRPLMSPSVFA
                     NKIFSMIKAQQKVLFCLIDNFRYDQWQAIKPLIIEENKVEQEEMYVSILPTATQYARN
                     SLFAGLMPYDIQQLYPQLWVNDDDEEGKNFNEAELLQHQLSRLGVKCQWQYEKILNQK
                     VSKKVASNFANFLKNDLTVLVFNFVDMLSHARTETEVIKELAADEASFRSVTRSWFQH
                     SVLPDIIKDAVSKGIKVVITTDHGTIKVTNPVKVIGDRHTSTNLRYKLGRNLDYNPKE
                     VFEVRKPAEVRLPSINLTSSYIFATDDDFLVYPNNYNHFVNYYRNTFQHGGVSLEEML
                     VPFIIIGKK"
     gene            complement(5113..7791)
                     /locus_tag="PK252_11865"
     CDS             complement(5113..7791)
                     /locus_tag="PK252_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT15448.1"
                     /translation="MSKFRYLFISILSVLLIFSSCSTKKNTIASRSYHNLTAHYNVYF
                     NGKESYKKGLKRLEQNYKEDYTVLLPVFQFPNAEAATNAVADMDRTIKKASKTIVLHS
                     ITAKPDIKKGIKTQKQKEFYNKREYNKWIDDAYLLMGKAYFMKLDLYNSNASFRQIIK
                     EYPKENTGIEAQIMLTRKLLFENEFSESADIINNLKTRKKFPKKYNSDLRAIQADYAI
                     RKKQYPEAAKMLEEALEFRNKKNQKLRYHFILGQLYQLNGQYDKSLEHFKKVIKLDPP
                     YEMAFNARINMANSFEAGSDASEIRRNLYKLMKDEKNKEFLDQIYYALGQIDLKEGKK
                     KEALDNYSKSCQYSTTNTKQKARSFLAMAKIYYADKEYLQSQKYYDSVLLNIDEFFPE
                     YPEINSRARNLQRLAKNLNTVSFEDSVQFVARMSENERNIFIDRIIATVKEKEAEEAR
                     KQQEEMMAEQTSMYEYSDMQARNQSSSINKDQSGKWYFYPSSKSYGENEFLVKWGKRK
                     LEDNWRRSNKKQMSFDESAQENTLQEVESEGKETDKKKVKDNKSREYYMQDLPLTDSA
                     MAASHDKIKKSLYNAAQIYKDDLSEYLLAAELYQDQAKRYPKDEATAQSLYQLFILYK
                     QIGENEKANMVKARLINDFPGNIYTQIVNNPEFVKQLQDKENQINEEYNKAYLAYQGG
                     DYQQALDISANMLANNPKHKIAIKFIFVKYLAEGKIYGLDSLRRGLNYIVSNYGKTEE
                     AVFAKSIIAQLDQTKPEIKKKEEIAQARAVYAKPEKDEKHLVIYIANDKKTNVNQLVF
                     NTINYNLDNFVRANLSVKAEQTSQNKQMVVIREFANMQEAINYYSAITRDKVILMKDV
                     TKEVDPVVISEKNYNIFKQDGNDSAYREFFNENYNK"
     gene            complement(7927..8181)
                     /locus_tag="PK252_11870"
     CDS             complement(7927..8181)
                     /locus_tag="PK252_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOT15449.1"
                     /translation="MGKGSVKEEKEIVEAINLSLEKIRPYLQADGGDVSFVGLTENMD
                     VKVRLMGACGHCPFSIQTLKAGVEQTLLNDIPQIKSVIAV"
     gene            complement(8246..10189)
                     /locus_tag="PK252_11875"
     CDS             complement(8246..10189)
                     /locus_tag="PK252_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOT15450.1"
                     /translation="MRNFFSKSIQAFKNQVSNNFVPSWIIFQIDLGINLFSIVAAYAL
                     RFNFQIPSEKSGIFQITIISIILIKSIIFYLSRTHVNVIRHSSIRDLFKIFTVVLFSS
                     IAVLVLNTIGYFIIQDFIVPLSVLLIDFFIAAYSMVMIRMIAKIFLNELKANNGVAVP
                     AIMVGVDELALSVKRAFDMDPETNYKFVAFIDTEKEIPKRMLDGIDIYNYKRIETIIR
                     RYNVKTIIIAKNQISPALKNHIVEKCLKHNVKVMSVSNVNSWVNGEVNVKQIKNIKIE
                     DLLERDPINLDTKEIRRQLENKTIMVTGAAGSIGSEIVRQIANFNPYKIILLDQGETP
                     LHNIELELDSTNNNFKAEIILADIANPTKLKQIFETHKIDVIYHAAAYKHVPMMERHP
                     SEAIKNNVYGTKLLADFAVKYKVPRFVMISTDKAVNPTNVMGASKRIAEIYVQSLNEF
                     GGTSFITTRFGNVLGSNGSVIPLFKKQIENGGPVTVTDPEVCRFFMTIPEACQLVLEA
                     SVMGKGGEIFIFDMGKSVKISDLAKNMIRLAGLVPGKDIEIKYTGLRPGEKLYEELLA
                     KEENTIPTYHPKIMIAKVQQYDFETVSLRINKLIQLAESSDNMQIVSMMKQIVPEYKS
                     HNSIFKVLDSEKKAKNIAQFEKS"
     gene            complement(10253..10849)
                     /locus_tag="PK252_11880"
     CDS             complement(10253..10849)
                     /locus_tag="PK252_11880"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT15451.1"
                     /translation="MFSGIVEEAAKVTRLKTENENLHITLSCSFTKELKIDQSVAHNG
                     VCLTVVDIRDNEYTVTAIKETLLKSNLGLLKLGDKVNLERSMLMNGRIDGHIVQGHVD
                     QTAECVEVVESGGSWYFTFKYDTAKGNVTVEKGSISVNGVSLTVVNSKSGSFQVAIIP
                     YTYEHTNFHTITKGSIVNLEFDIIGKYIAELLKRQQVI"
     gene            10945..12207
                     /locus_tag="PK252_11885"
     CDS             10945..12207
                     /locus_tag="PK252_11885"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT15452.1"
                     /translation="MQTIEKYNFKGKRAIIRVDFNVPLNKQTFEVTDDTRIRGALPTI
                     KKVLADGGSAILMSHLGRPDGKPQEKFSLKHIIPAVNKVLGVEVKFAGDCMGETAKQM
                     TASIKPGEVVLLENLRFYEEEEGKPYHLGENPTEEEKKAGKAKVKESQKAFVKQLASY
                     ADCYVNDAFGTAHRAHASTALIAEYFKNDSMFGFLIEAELKAMDKVLNEAQRPFTAIM
                     GGAKVSDKILIIENLLNRVDNLIIGGGMTYTFIKAQGGKIGNSICEADKLELALEILN
                     KAKAKGVKVYIPVDAVNADAFDANANTCISKTNETPDGWMGLDIADETIKQFSSVIEN
                     SKTILWNGPMGVFEMEKFAKGTSSIAQAIANATAKGAFSLIGGGDSVAAINKNKLADK
                     VSYVSTGGGAMLEYMEGKELPGIKAIRG"
     gene            complement(12245..12315)
                     /locus_tag="PK252_11890"
     tRNA            complement(12245..12315)
                     /locus_tag="PK252_11890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12281..12283),aa:Gln,seq:ctg)
     gene            12629..13579
                     /locus_tag="PK252_11895"
     CDS             12629..13579
                     /locus_tag="PK252_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT15453.1"
                     /translation="MKKFALIGAAGYIAPRHLKAIKDTGNMLVAALDKSDSVGILDSF
                     FPDTDFFTEFERFDRHIEKLRRNGQGVDYISICTPNYLHDAHIRFALRNHADAICEKP
                     LVLNPWNVEALKEIEKESGRKINTILQLRLHPVIMELKKVVESNKENKIHDIDLTYIT
                     ARGNWYFSSWKAVQEKSGGIATNIGIHFFDMLTWIFGNVEKNIVHIHGPRTAAGFLRL
                     KKANVRWFMSIDANTLPETVVKQGKRTYRSILINNKELEFSEGFTELHTQSYKEILEG
                     KGFSIEDVRPSIEIVHNIRNCKPVGLSGEYHPFVSTSQSI"
     gene            13688..16165
                     /locus_tag="PK252_11900"
     CDS             13688..16165
                     /locus_tag="PK252_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOT15454.1"
                     /translation="MFYLKNLLKCLFAFIVLSTTLVCGQDLKSVKVDELSDAQVEAIQ
                     KKALASGMTQDQLISAAQAKGMSADEIEKLKKRIDKLGKTNEKKATTTKRTRENKFEV
                     KQQKNKKDKKSTVEIDENDPFSVLLKEENPIDTTLKVFGSYLFTSKNLTFEPSLNIPT
                     PQNYQIGAGDEIIIDIWGASQQSYQLTVAPEGYIFIENVGPVYINGLTIDKASKQIIG
                     RLSTIYSGLKSYPQTTFAQVNIGSLRSIKVNLVGEVEMPGSYTLPSLATVFNALYAAG
                     GPSKNGTYRDIVVKRENKTVANVDLYDFLINGEQASNIRLQDQDVIIVRPYVSRIQLT
                     GEVKNPAIFELKETETLADLLKYAGGFTDKAYQNRLKITRKTDKEYAIADVAKEDYLS
                     FQMKDGDSIHVEPIINRFANKVEITGAVFRPGKYELTDKLTIKLLLDKADGLKEDAFT
                     ERAVLYRLESDLSKSSTSVNIKGIVNGSSADVELKNGDQLRIFSIFELEEEYDVKIDG
                     EIQLPETYPYFKNMTLSDLIATAGGFKESASLARIEVARRIKNDGVSVNDQISEIQLF
                     TVDKNLNLNNEASNFVLMPFDQVYVRRSPGYEEQTSAKVEGEVLYPGMYVIQNKEERI
                     SDLIKRAGGFTDRAYIKGAKLVRKLNIDEKERLEMIKRLKKQEKDTSNIEDVKIKDEE
                     AIGIDLQKIINNPHSKYDLLLQDKDKLIIPKELQTVKVSGALLYPNTVRYDRKNSMKN
                     YISMAGGFSEDAKKNKIYVIYANGSVDKTSSFLFFKFYPKVEPGAEIIIPKKSDKRKM
                     SPQESLGLITAMSSLALVLVAIVNATK"
BASE COUNT         5063 a         3153 c         2785 g         5169 t
ORIGIN      
        1 gcattcaagc caacctggta aagcctctcc gcctcagctt cagacgctcc gccaagcaaa
       61 gccccaattt tcatgcagcc agccagaaga acagacgttt tcaactttat catctccaga
      121 taatcctgtt cgctcacaga atcaagggtt tcaaagttca tgtcatactg ctgcccttcg
      181 catacttcaa gggccgtttg agtgaaagtc tttaataccg aaggcaacac agatacagga
      241 gactgcaaca tcaactcgta agccagaatg ctcatggcat cgccagacaa aatagcagta
      301 ttattgcccc acaaagcatg aatagtatct ttacctcttc tcaaaagggc attatccata
      361 atgtcatcgt gaactaaggt gtaattatga aaaacttcaa gcccaacagc aggattaata
      421 gcatttttaa tattttcaga aaataaatta caagaaagta aagtaataac gggccttata
      481 cgcttaccgc ctaatccaag aatatatttt gccggctcat aaagctcaac aggttttctt
      541 cccctagcaa cgttgaccag cgatttttca actataactc ttgcttgttc tgctgtgatc
      601 atttttttgc ctgccaaatt acatgttttt gtccaatcac gctattggtt ttctcaaatt
      661 tcacaatcat cataagtaaa taaattacat tttaacaacc caaaaacttc gtcaatcttt
      721 tttattattc actatcttag cggcaaaata tatttttatg aacgtattgt taattggttc
      781 cggaggtcgc gaacatgcta tagcctcaaa aattatccaa agcaaacatt tagaccagct
      841 ctttattacc cctggtaatg ccggtacagc caacattggc gtaaatattg agcttgcccc
      901 gtataatttt gaatctatag gcaaatttgc ctgtgccaat cgcgtcgata tggtagttgt
      961 aggccctgaa gagcctttgg taaacggttt ggctgattat ttccttcaaa atgaaaggtt
     1021 gaaaaacatt cctgtcattg gccctgtcaa agctggagct atgcttgaag gaagtaaaga
     1081 ttttgccaaa gaatttatgg taaaatacaa cattcctacg gctaaatatt ttacagtaac
     1141 ccaaaacaat attaacgaag gttttgcttt tcttcaaagc atgaatgccc cttacgtatt
     1201 gaaagccgat gggcttgctg ccggtaaagg cgtggtaata ctcaataaca ttgacgaagc
     1261 taaagccgaa ctggaagcta tgctttcggg gaaatttggt agtgcaagta ataaggttgt
     1321 tatagaacag ttcttaaaag gcattgagct ttctgttttt gtgcttaccg atgggcagtc
     1381 atacgtaata ctgccagaag caaaagacta caagcgggtt ggagaaggtg acactggcct
     1441 gaacacaggt ggaatgggtg ctgtatctcc tgtacctttt gcaaatactg aattcatgca
     1501 caaagtagaa caacgcattg ttattcctac tattgaagga cttaaaaaag aaaatattaa
     1561 atacaaaggc tttattttct tcggattaat taaagtggac ggcgaaccct atgttattga
     1621 gtataatgta agaatgggcg accctgaaac tgaagtagtt tttccccgca taaaaaatga
     1681 cgtacttgag cttttccagg ctgtggctaa tgaaacactg gctaaccaga aattggtcat
     1741 tgacgaacgc gttgctacaa cagtaatgct tgtttcggga ggatacccgg gtgattacaa
     1801 aaaaggttat gaaataaaca accttggcaa agtccaaaat agcatagtgt ttcatgcagg
     1861 tacaactcta aacgatggaa ttgtcaggac agcaggaggc agggtaattg ccattacttc
     1921 ttttggaaaa acaaaagaag aagctttgtt acaatcttat aaaaatgctg aaattattga
     1981 ttttaaagat aaatatttca gaaaagacat tgggtttgac ttataaaatg gtttgcagat
     2041 gcttttaaga gtttcacgtt ccagcaaccc ctccttacct attattctgt tgatactttc
     2101 attagtattt tggtttaacg ggtttacgga agtgtcttta aacaatcaaa tttaccattt
     2161 ttcacctttt tacgatttaa taaaattgct tccccaacaa gccggaatta ttcagtcatt
     2221 actgggaatt ctggctttat ggttcgttgc tttttcgctg atcaggataa attccaggtt
     2281 tatcattatc caggaaagaa cgtatctgcc agtacttttc tattttgtaa tgatatttgc
     2341 cattacaaaa attaactacc tgcatccggc attaattgcg gcaatattta ttagtattgc
     2401 tttagaaaga ttgttccata gcaataaaaa tgagaacctt tcgtacaacc cctttgatgc
     2461 tgcattcttt atcacgttag ctgcattctt ttatttcccg gcaatatttt tcaatgttat
     2521 cgtttggctt gcccttttaa tcatgcgtcc attttattgg agagaatggg tttattctat
     2581 tattggcatg tttgtgccat ctttcatcat attatcaata ttttacctta caggcaccaa
     2641 ctttcaatac attttaaaat cagcgaaact tcttttcact gttaaaaatg acatttcaat
     2701 aaccataacc gatgctttat ttttaagctt ttgcctggtt atgcttttaa tagccagtgc
     2761 ccacatgata gccatgatgg gcaagtttaa aattcttaca cgcaaggctt ataacttgtt
     2821 tcttgccctg ttccttgttt ctgtagttgt atatatcttc gtgccatcag taggcttaga
     2881 aataatattc attggagcat taccaataag ccaactcttt accaattact acctaaacgc
     2941 ccctgccaca aggttcagag agacagtatt tgacttgttt ataatatttt ttgtgcttac
     3001 ccagttgatt aagcaatagt tatcaaacgt ttttgcttgt tgtttacttc tatgtttact
     3061 tgtaaagtgt cgggcagaag tatattctca gctatttctg gccactctat taaacaaagg
     3121 ttgccactat aaaaatattc ttcataacca agatcatata gctcttccgc tttgtttaag
     3181 cggtaaaagt caaaatgaaa gatttttttc ccggaagcag taagatattc gtttactacc
     3241 gaaaaagtag gactggtgac attcccaaca acattcaggg ctttgcaaat ggtttttatc
     3301 agggtggttt ttcctgctcc cattttcccg gaaatagcta caactggctt atgagcagac
     3361 tttgaaatga tagattctgc aggtttgtgc aaatcctcca acgaagaaat ttcaaaactt
     3421 tccataatct gattttttag aaaacctaaa atcgaaaaac tctctaaact acaactgttt
     3481 caaaattctt tttgaaatag tatactacta ttaaatttaa gcaaaaaaag gcaaaagcgc
     3541 taattgccct ctcttatttt ttaccaataa taataaaagg caccagcatt tcttcgagcg
     3601 aaacgccacc atgctggaaa gtattgcggt aataattcac aaaatgattg taattattgg
     3661 gatacacaag aaaatcatca tctgtggcaa atatgtatga agaggtaagg tttatacttg
     3721 gcaacctcac ttcagccggt tttcttactt caaacacttc tttggggttg tagtcaaggt
     3781 tccgcccaag tttgtatctc aagtttgtag aggtatgacg gtctccaata acttttacag
     3841 ggttggtaac tttaatagtg ccgtggtctg ttgtaataac cacttttatc cccttcgaaa
     3901 cagcgtcttt aataatatcg ggcaaaaccg aatgctgaaa ccacgagcgg gttaccgagc
     3961 gaaacgaagc ctcatcggca gcaagttctt taattacctc ggtttctgta cgcgcatgcg
     4021 aaagcatatc tacaaaattg aacacaagta cggtcaaatc attttttaaa aagttggcga
     4081 agtttgatgc tacttttttt gatactttct ggtttagaat cttttcgtat tgccattggc
     4141 acttaacccc cagtctcgaa agctgatgct gcaataattc agcttcattg aaatttttac
     4201 cttcttcatc atcgtcatta acccaaagtt gaggatataa ttgctgaata tcatagggca
     4261 taagccctgc aaataaagaa ttacgggcat attgtgtagc tgtaggaaga atgctaacgt
     4321 acatttcttc ttgctcaact ttattttctt caataattaa tggctttata gcttgccatt
     4381 gatcatacct gaaattgtca atcaggcaaa ataagacttt ttgttgtgcc ttgatcattg
     4441 aaaatatttt gttggcaaat actgaaggag acatcaaagg cctatcctga gcgttttctt
     4501 taaaccattt ggcatagctg ctttttataa acctcccaaa ttcatggtta gcctcacttt
     4561 tctgattttt cagaacctca gacaatccct gtcctgtttt ttccagttca agttcccaaa
     4621 aaacaaggtt tttgtatatt tctttccaat cttcaatgct ttgtgctgaa tttatgcgtt
     4681 ggcttatttg cataaactgc gtttggtagg tttgcgttgt tttactgcta accaaccgct
     4741 tattatcaat atgtttcttt aatgtcaata acacttgatt aggattcaca ggctttatca
     4801 gataatcatc tatctgcgaa ccaatggcat cttccatcag gttttcttcc tcacttttgg
     4861 ttatcataac aacagggact ccgggcaaaa tccgctttaa aggctcaata agctctaatc
     4921 cgcttttgcc gggcatgttt tcgtccagaa acacaatgtc aacagtacct ttttgagcaa
     4981 tttgcaatgc atcatcaccg ttagttacag atataatctt atatccacgg ttctcaagaa
     5041 ataaaatatg gggctttaat agttctattt catcgtcagc ccaaagaatg gttatttccc
     5101 gtgtcataga cgttatttgt tgtagttttc attgaaaaac tcacggtatg ccgaatcatt
     5161 cccatcctgt ttgaaaatgt tgtagttctt ttccgaaatt acaacaggat caacctcttt
     5221 tgtaacatct ttcatcaaaa tcaccttatc tctcgtaata gccgaataat aattgatagc
     5281 ttcctgcatg ttggcaaatt ccctgataac aaccatttgt ttattttgcg acgtttgttc
     5341 ggctttaaca ctaaggtttg ccctcacaaa attatcaaga ttgtaattta tagtattaaa
     5401 taccaactgg ttaacatttg tctttttatc atttgcgata taaataacca aatgtttttc
     5461 atctttttcg ggcttagcat aaacagccct tgcctgcgct atttcttctt ttttctttat
     5521 ttcaggcttt gtctggtcta actgggctat aatgcttttg gcaaaaacag cctcttctgt
     5581 cttaccataa tttgaaacaa tatagtttag gcctcgccta agcgaatcca acccatatat
     5641 tttgccttca gccaaatatt taacaaagat aaacttaata gctatcttgt gtttagggtt
     5701 attggcaagc atatttgccg aaatatccaa cgcctgctga taatcccctc cctgatatgc
     5761 caaataagcc ttattgtatt cttcatttat ctggttttct ttatcttgca attgcttaac
     5821 aaactcagga ttattaacaa tctgggtata aatattgccc ggaaaatcat ttataagcct
     5881 tgccttgacc atattggcct tttcgttttc cccaatttgt ttataaagaa taaaaagctg
     5941 gtacaatgat tgtgctgttg cttcgtcttt ggggtacctc ttagcctgat cctgataaag
     6001 ttctgctgct aacaaatact cagacaagtc atctttatat atctgagctg cattatacaa
     6061 cgacttttta atcttatcgt gcgaagctgc cattgccgag tctgtcaatg gtaaatcttg
     6121 catatagtat tcgcgtgact tattatcttt aaccttcttt ttatccgtct ctttaccttc
     6181 tgattcaact tcctgcaaag tattttcttg tgcagattcg tcaaaactca tttgtttttt
     6241 gttacttctc cgccagttgt cttccagctt tcgcttcccc catttgacca aaaactcgtt
     6301 ttcgccatag ctctttgatg aagggtaaaa ataccatttg ccactttggt ctttatttat
     6361 cgaactggat tgattgcgcg cctgcatatc ggaatattcg tacatactgg tttgctcagc
     6421 catcatctct tcttgctgtt tgcgggcttc ttctgcttct ttttctttta ccgtggcaat
     6481 aattctatca ataaaaatat tccgctcatt ttcagacatc ctggccacaa actgaacact
     6541 atcttcaaaa cttacagtat ttaaattttt tgccaaacgc tgtaaattcc tggcccgact
     6601 gttaatctcc gggtattcgg gaaaaaattc gtcaatattc agcaaaacgc tatcatagta
     6661 tttctgcgac tgaagatatt ctttgtctgc ataatatatc ttggccatgg ccaaaaaact
     6721 tcttgctttt tgcttggtgt ttgtagtact gtactgacaa gattttgaat aattatcaag
     6781 agcctctttt ttcttgccct ctttcaaatc tatctgtcct aaggcgtaat atatctgatc
     6841 aagaaactct ttattcttct catctttcat cagtttataa aggtttcttc tgatttcaga
     6901 agcatcagag cccgcttcaa atgaattggc catattgata cgggcattaa atgccatttc
     6961 gtaaggcgga tccagcttta ttactttttt aaagtgctct aaagacttat catactgccc
     7021 gttaagttga tacaactggc ctaaaataaa atgatacctc aacttttgat ttttcttatt
     7081 cctgaattct aaggcttctt caagcatttt cgctgcctca gggtattgtt tctttcgtat
     7141 tgcataatcg gcctgaattg cccttaaatc tgagttatat tttttaggga actttttcct
     7201 ggttttaaga ttattaataa tatctgcaga ttcggaaaat tcattttcaa aaagcagctt
     7261 acgcgtgagc ataatttgtg cctctattcc ggtattttct ttagggtact ctttgattat
     7321 ctgcctaaat gatgcattag aattgtacaa gtcaagtttc ataaaataag ccttgcccat
     7381 taaaagatag gcatcatcaa tccatttatt atattcgcgt ttattataga actctttttg
     7441 cttctgcgtt tttatcccct tctttatgtc cggctttgcc gtaattgaat gtaaaacgat
     7501 tgttttagaa gcttttttta tcgtcctgtc catatctgct acagcattgg ttgctgcttc
     7561 tgcatttgga aactggaata caggcaataa tactgtataa tcctctttgt agttttgctc
     7621 aagacgcttt aagccttttt tatagctttc tttaccattg aagtaaacat tgtaatgcgc
     7681 agtaaggtta tgatatgacc ttgaagcaat tgtgtttttc tttgtcgaac aactgctaaa
     7741 aatcaataaa acagataata ttgaaataaa taaatatcga aatttgctca taaaccggtt
     7801 tatagacaaa aaacttgttt attggtaaaa tattgtaagc atttaaataa aaaagaggat
     7861 ataaatataa tcctctacat tatcccggta aagatacaat gattttaaaa actttaagaa
     7921 aaagtattaa acagctatga cagattttat ttgaggaata tcatttagca gggtctgctc
     7981 taccccggcc ttgagcgtct gaatactgaa cggacaatgc ccgcatgctc ccatcaacct
     8041 tacttttaca tccatatttt ctgtcaaccc gacaaatgaa acatctcccc catcagcctg
     8101 aagatatggt cgtatttttt caaggctaag attaattgct tctacaattt ctttttcctc
     8161 tttgacagaa ccttttccca tgtacctgtt tttctaaaca aacaattgat attctataat
     8221 gttcataaca aagacccaaa atgttctaac tcttctcaaa ctgggctatg ttttttgcct
     8281 ttttttcaga gtcaagtacc ttgaatatag agttgtggct cttatactca ggaactattt
     8341 gcttcatcat cgatactatt tgcatattat ctgatgactc ggccagttga attaatttgt
     8401 ttatacgtaa tgacaccgtt tcaaaatcat attgctgtac cttcgcaatc ataattttgg
     8461 ggtgatatgt gggaatggta ttttcttctt tagcaagaag ctcttcataa agtttttctc
     8521 ctggacgtaa gccggtatat ttaatttcaa tatccttgcc tgggactaac cccgctaaac
     8581 ggatcatatt tttggctaaa tcagatattt ttacagactt gcccatgtca aagataaata
     8641 tctctcctcc cttacccatg acgcttgcct caagtaccaa ctggcatgct tctggaatcg
     8701 tcataaagaa acggcatact tcaggatctg taaccgttac tggcccacca ttttcaattt
     8761 gttttttaaa caatgggata accgaaccat ttgaacccaa cacattgcca aacctggtag
     8821 taataaaact ggtacctcca aattcattca acgactgaac ataaatttca gcaatccgtt
     8881 ttgaagctcc catgacattt gtagggttga ccgccttatc tgtagaaatc ataacaaaac
     8941 ggggtacttt atactttact gcaaagtctg ccagcaactt tgtcccatat acattgttct
     9001 ttatcgcttc cgaaggatgc cgctccatca ttggcacatg cttatatgcc gcagcatgat
     9061 agatgacatc aattttatgg gtctcaaata tttgtttaag ctttgtaggg ttggcaatat
     9121 ctgccaatat aatctctgcc ttgaaattgt tgtttgtact gtccagctca agttcaatat
     9181 tatgcaaagg tgtttctccc tgatcgagaa gaataatttt atacgggttg aagtttgcta
     9241 tttgccttac tatctcactc cctattgatc ctgcagcccc ggtaaccata atggttttat
     9301 tttcaagctg gcgacgaatc tctttggtat caagattgat agggtccctt tccaacaaat
     9361 cctcaatctt gatgttctta atttgtttta cattcacctc gccgtttacc caactattga
     9421 catttgaaac tgacataact ttaacgttat gcttaagaca tttttcaaca atatgatttt
     9481 ttaaggctgg cgaaatttga ttttttgcta ttataatggt ctttacgtta taccttctta
     9541 ttatagtttc tatcctttta taattataaa tatcaatgcc gtcaagcatt ctcttgggaa
     9601 tctctttttc tgtgtctata aatgcaacaa atttataatt agtctccggg tccatatcaa
     9661 aagccctttt tacagacaat gccagttcat ctaccccaac cataatggca ggaactgcaa
     9721 ctccattgtt tgcttttagt tcgttaagaa atattttggc tatcatacgg atcataacca
     9781 tagaatatgc agcaataaaa aagtcaatta ataaaactga caagggtaca atgaaatctt
     9841 gtattataaa ataccctatg gtgtttaata ccaaaactgc tatgctggaa aataaaacaa
     9901 ctgtgaatat tttaaataaa tctcttattg aagaatgcct gattacatta acatgcgtcc
     9961 ggcttagata aaaaattata gattttataa ggattataga aattattgta atttgaaaaa
    10021 taccagattt ctcagagggt atttgaaaat tgaaccttaa agcataagct gcaacaatag
    10081 aaaataaatt gatgcctaaa tctatttgaa agattatcca ggatggtaca aaattgttcg
    10141 aaacctggtt tttaaaagct tgtattgatt ttgaaaaaaa atttctcacc tgaaactgat
    10201 tgaatttttg cttaaattta ctcaattatt gacaataaaa caaaaacaat tgtcatatta
    10261 cttgttgcct tttcagcaac tctgcaatgt acttcccaat aatatcaaac tctaaattaa
    10321 cgatagagcc cttagttatt gtatgaaagt ttgtatgttc ataagtataa ggtataattg
    10381 ccacctgaaa gctgcccgat tttgagttta caacagttaa actcacccca ttgacagaaa
    10441 tactaccctt ctcgacagta acattgcctt tggccgtatc atacttaaaa gtaaaatacc
    10501 agcttcctcc actctcaaca acttcaacac attcggcagt ctggtcaaca tgcccctgaa
    10561 caatatgccc gtcaatccgg ccattcatta acatgcttcg ttcaaggttt actttgtcac
    10621 caagcttcag cagccccaaa tttgacttca acagtgtttc tttaatggca gtgaccgtat
    10681 attcgttatc cctgatgtca acaaccgtta agcatacccc attatgagcc acactttggt
    10741 caattttcaa ttctttggta aaactgcatg aaagagtaat gtgcaagttt tcattctccg
    10801 ttttcaatct cgttaccttc gctgcttctt ctacaatccc tgaaaacata gcggttatcc
    10861 ttgaggttag gtattatttt agtgctacaa aagtaataaa tactttttat atctttgcat
    10921 attcatttaa aatactcaat tattatgcaa actattgaaa aatacaattt taaaggtaaa
    10981 agagccatta tcagggttga ctttaatgtc cctctcaaca aacaaacatt tgaagtaact
    11041 gatgacaccc gtatccgcgg agctcttcct acaattaaaa aagttctggc tgatggaggt
    11101 tctgccattt taatgtctca tcttggacgt ccggatggaa aaccccagga aaaattttct
    11161 ttaaaacata ttattccggc agtaaataaa gtccttggcg tagaagtaaa atttgccggc
    11221 gactgtatgg gagaaactgc taagcaaatg acagcatcta tcaaaccagg agaagttgtt
    11281 ttgttagaaa atctccgttt ttacgaagaa gaagaaggta aaccatacca tttaggcgaa
    11341 aatcctacag aagaagaaaa aaaagccgga aaagcaaaag ttaaagaaag ccaaaaagct
    11401 tttgtaaaac aactggcaag ttatgcagat tgttatgtaa atgatgcatt tggcactgct
    11461 caccgtgccc acgcttcaac agcccttatt gctgaatatt tcaaaaacga cagtatgttt
    11521 gggttcctaa ttgaagccga acttaaagca atggacaaag tgttgaatga agctcaacgt
    11581 ccttttacag ctattatggg tggggcaaaa gtgtctgaca aaatccttat tattgaaaac
    11641 ctgcttaacc gtgttgacaa cctgattatt ggtggcggga tgacttatac tttcattaaa
    11701 gcccaagggg gtaaaattgg taattcaata tgtgaagcag acaaacttga gcttgccctt
    11761 gaaatattaa ataaagctaa ggctaaaggc gtaaaagtat atataccggt agatgctgtt
    11821 aatgccgacg cttttgatgc caatgctaat acttgcattt ctaaaacaaa tgaaacccct
    11881 gacggatgga tggggcttga cattgcagac gaaactatca aacaattcag cagtgttatt
    11941 gaaaattcaa aaactatttt atggaatggc ccaatgggag tttttgaaat ggaaaaattt
    12001 gccaagggaa cctcttcaat agcacaagct attgctaatg caacagctaa aggcgccttc
    12061 agcctaatcg gcggtggcga ctctgtggct gctatcaata aaaacaaact agcagataaa
    12121 gtaagttacg tttcaaccgg cggtggtgct atgctcgaat atatggaagg caaagaactc
    12181 cctgggataa aggctatcag aggataaaaa tgacaaagcc ccgcaaaacg gggctttatt
    12241 tttgttgtcc tgctaggact cgaacccaga caagcagaac cagaatctgc ggtgctacca
    12301 ttacacaaca ggacagcttt tctgctgcaa aaataataca atatttttta aacagctttt
    12361 attattttaa tttttccact aaatctgtct tataataatt gtagggacca gagttgccgt
    12421 ttttattgaa ctttatctcg tctcaagatc cccagaaaac attctcgcaa aactacagcg
    12481 ggctttacaa aaaactctga agcccctgcg gttaagtctt tatctccaat acaactaatt
    12541 tacccaaaga atttacaaat tggcataagg cttataacaa taaatactat ttttgtggcg
    12601 ttttaaagct gaccaataac aaccgcagat gaaaaaattt gccctcatag gtgcagcagg
    12661 ttatatcgcc cctcgtcacc ttaaagccat caaagataca ggcaacatgc ttgttgcagc
    12721 ccttgacaaa agtgacagcg taggtatttt agatagtttt ttccctgata cagatttttt
    12781 caccgaattt gagcgctttg acaggcatat cgaaaaactg cgcagaaatg gccaaggagt
    12841 tgattatatc agcatttgca ctccaaacta tctgcacgat gcccacatta ggtttgccct
    12901 tcgtaaccat gccgatgcta tttgtgaaaa accccttgtg cttaacccat ggaatgttga
    12961 agcattaaaa gaaattgaga aagagagcgg tagaaagata aataccattc ttcaacttcg
    13021 tttgcaccct gttatcatgg aattaaaaaa agtagttgaa agtaacaaag aaaataaaat
    13081 acacgacatt gacctgactt atatcactgc aagaggaaat tggtactttt ccagctggaa
    13141 agctgtacag gaaaaatcag gcggcatagc caccaacatc ggaatccatt ttttcgatat
    13201 gctgacctgg atttttggaa atgtagaaaa aaatatcgtt cacatacatg ggccacgcac
    13261 agctgcaggt tttttaaggt taaaaaaagc caatgtaagg tggtttatga gtattgatgc
    13321 caatactttg ccagaaacag ttgtaaaaca agggaaaaga acttatcgct cgatattgat
    13381 aaacaacaaa gaacttgagt tcagcgaagg ctttactgaa cttcatacac aatcatataa
    13441 agaaatattg gaagggaaag ggtttagtat tgaagacgtc cgcccaagca ttgaaattgt
    13501 tcataatatt agaaattgta aacctgtcgg attaagcgga gaatatcacc catttgtatc
    13561 taccagtcaa agcatataag tgtataaaca acattttatt gttaaaaaca attttgccta
    13621 ttagactttt ttttacatat cttaaaatta tttacttttg cccactaaat taaaatttaa
    13681 tataagaatg ttttatctga aaaacctcct aaagtgttta tttgccttca tcgttttgtc
    13741 aacaacgttg gtttgcggac aagatttaaa aagcgtgaaa gttgatgaac tttcagatgc
    13801 acaggtcgaa gccatacaaa aaaaggcttt ggccagcggg atgacgcaag atcaattaat
    13861 ctcagcggcc caggcaaaag ggatgtctgc tgacgaaatt gaaaaattaa agaaaagaat
    13921 agataaattg ggtaaaacca acgaaaaaaa ggccacaacc actaaaagga caagagaaaa
    13981 caaatttgag gttaaacagc aaaaaaacaa aaaagataaa aaaagcactg ttgagattga
    14041 cgaaaatgac ccgttttcgg ttttgctgaa agaagaaaat ccaatagaca caacactaaa
    14101 agtatttggt tcatatttat ttacttccaa aaacttaaca tttgagccaa gcttaaatat
    14161 tcctaccccg caaaattacc agattggtgc tggtgacgaa attatcatcg atatctgggg
    14221 ggcttcccaa caatcatatc agcttacagt tgcccccgaa gggtatatct tcattgaaaa
    14281 tgtagggcct gtgtatatca atgggctaac cattgataaa gcttcaaagc aaattattgg
    14341 acgattatcc actatatatt cagggttgaa aagttatcct caaacaactt ttgcacaggt
    14401 caacattggc tctttacgga gcataaaggt caacctggtt ggagaagttg aaatgccggg
    14461 ttcttataca cttccttcat tagcaacagt gtttaatgca ctatatgcag ccggaggccc
    14521 ttctaagaac ggtacatata gggatattgt tgtaaaaagg gaaaataaga ctgtcgctaa
    14581 tgtggactta tacgattttc ttattaacgg agaacaagcc tccaatatac gcttgcaaga
    14641 tcaggatgtt attattgtca ggccttacgt ttcaaggata caactaaccg gagaagtcaa
    14701 aaatccggct atttttgagt taaaagaaac cgaaaccctg gctgatttgt taaaatatgc
    14761 cggaggtttt acagacaagg cttatcaaaa taggttgaaa ataacccgta aaactgataa
    14821 agaatacgct atagctgacg ttgcaaaaga agattatctt tcattccaaa tgaaagatgg
    14881 cgacagtatt catgttgaac caattatcaa ccgttttgcg aacaaagttg aaataaccgg
    14941 ggctgttttc agaccgggga agtacgaatt aactgacaag ctcactataa aactactgct
    15001 tgacaaagca gacggcctta aagaggatgc atttaccgaa agggctgtct tgtaccgctt
    15061 agaatcagat ttgtcgaaaa gttctacttc tgtcaatatt aaaggaatag taaatggctc
    15121 ctctgctgat gttgaactga aaaatggtga ccaactaagg attttctcca tttttgagct
    15181 ggaagaagag tacgatgtaa aaattgacgg agaaattcaa ttgcctgaga catatcctta
    15241 ttttaaaaac atgacattgt cagatcttat tgccactgcc ggaggcttta aagaatctgc
    15301 atcgttagca aggattgaag tagcccgaag gataaaaaat gacggagttt ctgtcaatga
    15361 tcaaatcagt gaaatacagt tatttactgt agataaaaac ctgaatctga acaacgaagc
    15421 ttctaacttt gtactaatgc cttttgacca agtttatgtt cgtcgttcgc ctggttatga
    15481 agaacaaaca tctgccaaag ttgaagggga agtattatat cccggcatgt atgtcatcca
    15541 aaacaaagaa gaacgtattt ctgacctcat taaacgtgca ggtggtttta cagacagggc
    15601 ttacattaaa ggggccaaac ttgtcagaaa acttaatatt gatgaaaaag aacgccttga
    15661 aatgattaaa cgcctcaaaa aacaagaaaa ggacacctcc aacatagaag atgtaaaaat
    15721 taaagacgaa gaagccatcg gtatcgacct gcaaaaaata ataaacaacc cacattcaaa
    15781 atatgacttg ctgttgcaag ataaagataa attaatcatc cccaaagaac ttcaaaccgt
    15841 gaaagtatct ggagcattgc tatatcctaa tacagtaaga tatgaccgga aaaattctat
    15901 gaaaaattac atttccatgg ccggaggctt ttcggaagat gccaaaaaaa ataaaatata
    15961 tgtaatatat gccaatggtt ctgttgacaa aacatctagt ttcctgtttt tcaagtttta
    16021 cccgaaagtt gaacccggtg ctgaaatcat catccccaaa aaatcagata aacggaaaat
    16081 gtctccccag gaatcattgg gtttaataac tgccatgtca tcactggcct tagtgcttgt
    16141 agctattgta aacgcaacca aataatttta
//
LOCUS       DAOSPF010000069        14741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4099_length_14741_cov_7.849993, whole genome shotgun sequence.
ACCESSION   DAOSPF010000069 DAOSPF010000000
VERSION     DAOSPF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_14741_cov_7.849993"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..450)
                     /locus_tag="PK252_11905"
     CDS             complement(34..450)
                     /locus_tag="PK252_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HOT15455.1"
                     /translation="MTRNKRKQEIEGEVWAIIPDTDSKYEISNFGRVKSYCQNSKDGK
                     ILKPSLIGGFEVVSLKCKEGARHFLVHKLVAELFVDKIEGDESVVIHLDWNKRNNHFT
                     NLMWHTKENSYKRMHERLQNERKKKGKVVTPDFVGI"
     gene            822..2144
                     /locus_tag="PK252_11910"
     CDS             822..2144
                     /locus_tag="PK252_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15456.1"
                     /translation="MQIILTIFFITIFFYPLIGQEEAIPFDTKGKIWVIDKKVIKTIK
                     LFKDEEVIEARLFTINDSISVFEKLIKKDDKTFRDKQILSNSELNSLRAKIDSISLMV
                     INQEGRSWLLASSTFLGLTLYGPSVPSIINSTNDKIQVSSYMLTAASGFFLPWYLTRN
                     RSVSYAHLNLTTFGLSWGFLHGAQIYWATSGYNNLNNALTFGTICGITEGFIGYNLVD
                     KFNISYGQAQLMTFYSEIGLFIGFAAAQRFNIDAEEKPGGFQGLSLLGSALGLGIGYR
                     QGLDKSITAGDAQIIGISALLSAYLTIPPLSDIAPDDSRNISTTLSLAGICGAFIGSK
                     LIRNIDFSYGQSLMIGLGTIAGGFTGAGVGYLIDNNSSLPIYISGLGALTSFGLMFNY
                     YKNKSETSYEKNNFRINLYPQNYLVWNRANLQPIYLTNLPLVSISKTF"
     gene            2209..2718
                     /locus_tag="PK252_11915"
     CDS             2209..2718
                     /locus_tag="PK252_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT15457.1"
                     /translation="MNYLDVLIIILLIIAAVSGFKKGFVHQLFSLLAIILGILLAVKL
                     NTWLTGLITPGIFSSVQTAKIVAFIFVLIVVSILAHLIAKILESFFNDIDLGLINRLT
                     GLIFGVLKMSFILSAFMILVEFSIIKINFPKKESCDSSFLYPKVKVIAPAVFPYLKTI
                     TVEMSKTFH"
     gene            2737..4509
                     /locus_tag="PK252_11920"
     CDS             2737..4509
                     /locus_tag="PK252_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT15458.1"
                     /translation="MFWDKIVAWLHAIRLKYVEKKAYVIFVSIIIGIFSALAAVMLKN
                     IVYYIKILLQYDWGISYLSILYFIYPLVGIFIVYIYLRVFHDGHINKEIGYLIYKLSR
                     NENDLPRRQIYAHLITSGTTVGFGGSVGLEAPIVLSGSSIGSAISKYFRVNLTDRNLL
                     IACGAAGGISAIFNAPIAATIFAFEVLLPSMAVSAFVPLLISSATASIFSNLLFAEKP
                     FVLITNAWEVNDLTFYIVFGVLCGLLASYMIKMSYWIGRMIFRNIKSQVSRLIIGSIL
                     LGSMIFIFPALYGEGYDMVNALFVNNVNLVIISPLFQSLPTNTWIILIAITIILLIKV
                     IATSLTLGAGGNGGIFAPSLFTGAFAGYAFATIINLTGVAHVNAICFMAVGMAGVFSG
                     VLKAPMTAIFLIAEVTGGYSLFLPLMIVSALSYFIARYIEPHSVYTKMLAEDKKWDHE
                     KIDSVMLQRLSIKDLIETNFTCITIDDSLFSIREKVFSSSHKFFPVVDKDLHLTGIVH
                     FNDIKNFFLNKHEEDFGWEMLIKPVKDKIEINDSVEEALKKLENVGAWNLPVTDNGIY
                     KGFLFRSRILDSYRQHVIQQRTMF"
     gene            4595..6385
                     /locus_tag="PK252_11925"
     CDS             4595..6385
                     /locus_tag="PK252_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15459.1"
                     /translation="MGKIILNYHDYFKKDLRLIFIITVILSCIGNIADINVVMAHSNY
                     ALYINFANVFVNLIALLLFFRFKVPVRICLGIVIYAVIINLMGSQLYDFPKSNSVMYL
                     LRATLIIALAVTVAAICISKMHSFILSGLFIAFYSYLAISTNNKFLIDNFLIICIMFL
                     IYNVVTYLFVRNIHNSIMQLQEQNAVITEQKESLEEAYWNQEESRILLNKKNEELTIA
                     KNKAEESDRLKSAFLANMSHEIRTPMNGILGFANLLKKPSLSGEKLNKYISAIELSGK
                     RMLNIIDDLIDISKIEAGQIDIKVEKTSIYQVVNELCVFFKPEADNKKIELQIAIDRN
                     DHECFIKTDKTKLLQILTNLIKNAIKFTHEGSIQIGYKIKGRVVEFFVKDTGIGIPEY
                     MHEKIFERFLKADNTLIKPYEGAGLGLAISKAYVNMLGGEIWLTSKENEGTTFFFTLP
                     LISGNANNVSNNVVDFYPLDQVVSIKSNKTILIAEDDEISYMYLSEFLDGSGITIIRA
                     EDGQKAVDLVQQMPNLDLVLMDIKLPQMNGHEALSLIRKIRPHLPIIAQTAFAMQSDI
                     DRYISEGFNDYLTKPIQIENLNNILAKYLQ"
     gene            6527..6907
                     /locus_tag="PK252_11930"
     CDS             6527..6907
                     /locus_tag="PK252_11930"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT15460.1"
                     /translation="MAKVSEEFDSTAINLLGAGTSINGDINSNGDIRINGTVSGNITT
                     KGRLIIGESGKIKGDISCRNADILGYFEGNIQVTELLSLKSSSSITGELNVGRLAIEP
                     GCKFNGTCKMNDNSPVAAENKDAK"
     gene            6897..7109
                     /locus_tag="PK252_11935"
     CDS             6897..7109
                     /locus_tag="PK252_11935"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOT15461.1"
                     /translation="MRNDNNWMRNYVRYSSLMLQMIIIIVVGVYGGYRLDLSFSDGKH
                     WFLILLTIVSTFVALYLFIKDLLKKG"
     gene            7580..8698
                     /gene="atpB"
                     /locus_tag="PK252_11940"
     CDS             7580..8698
                     /gene="atpB"
                     /locus_tag="PK252_11940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT15462.1"
                     /translation="MARSITFATIISFMLLSFNMYGQHITADTAISHDSVVTEKAHNP
                     EGGFKPGDFMLEHILDSHEWHIITVGHTHVSIPLPVILYSKVSGLHFFMSSKFHHGTE
                     SYKNFYIAEEGPNKGKIVEKVQSEHGEKEIVPLDLSITKVVFSLLLSCILLLVVFISI
                     ARKYTREGIKAPSGLQSALEPMILFVVDDIAKPSLGEKKYEKFLPYLLTIFFFIWFNN
                     MLGLIPIFPGGANVTGNIAVTMVLAAFTFVITTINGNKHYWEEIYNAPGVPWWLKFPI
                     PLMPFIEILGLIIKPVVLMVRLFANITAGHLIILAFVSLIFIFAQISPALGYGVSVLS
                     TAFALFMSLLELLVALIQAYVFTLLSAIYFSMATAEHH"
     gene            8722..8973
                     /gene="atpE"
                     /locus_tag="PK252_11945"
     CDS             8722..8973
                     /gene="atpE"
                     /locus_tag="PK252_11945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT15463.1"
                     /translation="MLTALLILLEAAGVAKLGAGVGAGLAAVGAGLGIGLIGGNFMEA
                     IARQPEAVGDLRSNMIIAAALVEGVAFFAIIVCLLILFL"
     gene            9052..9543
                     /locus_tag="PK252_11950"
     CDS             9052..9543
                     /locus_tag="PK252_11950"
                     /inference="COORDINATES: protein motif:HMM:NF011041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT15464.1"
                     /translation="MELVTPNVGTIFWMLLFFSLVLLILKKFAWKPILNSLKNREKSI
                     EDALKSAEHAKEEMQKLKQDNEKILADARRERDFLMKEAREMKDKIIADAQELAKVEA
                     KKLIDAAKQNIDNEKRNAINEIKMQIANLSIEIAEKIIREKLDEEKQKQIVEKMLDEI
                     NLS"
     gene            9555..10100
                     /gene="atpH"
                     /locus_tag="PK252_11955"
     CDS             9555..10100
                     /gene="atpH"
                     /locus_tag="PK252_11955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOT15465.1"
                     /translation="MNESIIAVRYAKALFQLAVEKNKADVLKNDMETLYHSIEAVSEL
                     KALFYNPVVKPGMKTNIIREVFGSFDADTLGFLTLLIQNRREEYIEEIALNFIKRYRK
                     YKGIEKVIFTTAIKIDGDIESKIKQQIGKYLKADVEFESQVDERLIGGFRLRLGDKQY
                     DASISTKLEDLKRKLVKTSIH"
     gene            10122..11702
                     /gene="atpA"
                     /locus_tag="PK252_11960"
     CDS             10122..11702
                     /gene="atpA"
                     /locus_tag="PK252_11960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT15466.1"
                     /translation="MANIKPSEISGILKKAIEGAGAGVAMEEVGTVLQVGDGIARIYG
                     LSQVQSNELIEFANGMKGIVLNLEEDNVGAVLLGPSTEIREGDTVKRTKRIASINVGE
                     GMLGRIINTIGEPLDGKGPITGKTYEMPLERKAPGVIYRQPVKEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFFDKGTPVYCIYVAVGQKGSTVANIAKT
                     LEEHGAMHYTTIVAATAADPAAMQFYAPFAGCAIGEYFRDTGRPALIVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIESDEIAAQMNDLPETLKPIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYKNLEAFAKFGSDLDAATLAILDKGAKNVEILKQGLH
                     SPLTVEKQIAIIYCGTNGLLKDIPLSKVKEFEFEFLQHIEMKNPEVLSKLRDGIINED
                     ISKTIEKLAAEVVDKLKS"
     gene            11730..12614
                     /gene="atpG"
                     /locus_tag="PK252_11965"
     CDS             11730..12614
                     /gene="atpG"
                     /locus_tag="PK252_11965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOT15467.1"
                     /translation="MANLKEIRTRIASVTSTKQITSAMKMVSAAKLKKAQDAVLQLRP
                     YADKLSNILKDVSDSCDNITENVYIKPRPVKNVLVVVITSNRGLCGGFNANAVKLVMN
                     LAQNEYNNLFKTNRFKIFCIGKKGNDMLKSKKMPIYQYRADLVDKPTYARIEDTANDL
                     LEWYKSGEFDKIILIYNRFKNAAVQILAEEQFIPVLLEKEKEKKRGILSDYIFEPQQA
                     ELVDELIPYTLKIRFYNAILDSLASEHGARMTAMHKATDNASELIKELTLSYNKARQA
                     TITGEILEIVGGAEALKG"
     gene            12640..13053
                     /locus_tag="PK252_11970"
     CDS             12640..13053
                     /locus_tag="PK252_11970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT15468.1"
                     /translation="MYTYFISPKFGDIDGLGHVNNTVIPSWFEQARNYLYKLFNPELD
                     LKTWNLILARFEVDFVSQMYYSKDVEIRTFVSKIGRSSFEVYQEAWQSGHICAKGKTV
                     LVHFDFIQQKSAAIPEDIREKLSEHLIAAIDATAN"
     gene            13131..14423
                     /gene="tyrS"
                     /locus_tag="PK252_11975"
     CDS             13131..14423
                     /gene="tyrS"
                     /locus_tag="PK252_11975"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT15469.1"
                     /translation="MDFFEELKWRGMIHDYTPGLEAQLQREMTTAYVGIDPTADSLHI
                     GHLVSIMLLKHLQVAGHKPIALVGGATGMIGDPSGKSEERNLLSLETLRHNQECIKKQ
                     LSRFLNFETQEKNCAILVNNYDWMSTFSFLDFIRDVGKHITVNYMMAKDSVKKRISGE
                     DREGMSFTEFTYQLVQGYDFLYLYKNYGCKLQMGGSDQWGNITTGTEMIRRIEGGEAF
                     ALTCPLITKADGGKFGKTEKGNVWLSSAYTSPYQFYQFWMNVSDADAEKYIKIFTMLP
                     PAEIEDIIEKHKVEPHLRLLQKALAKEVTVMVHSQAEYESAVEASEILFGKGTAETLA
                     NLSENDFLAIFDGVPRFEITREVLKSNIGILDLLASSTAVFPSKGEAKRLIEGGGLSI
                     NKNKVNSANDTVNESFLLNEKYLLVQKGKKNYNLIIVK"
     gene            14438..>14741
                     /locus_tag="PK252_11980"
     CDS             14438..>14741
                     /locus_tag="PK252_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOT15470.1"
                     /translation="MTKKDKFEFDATSIIKFTLKNIKLLIAVSIVAFIVSAVVSFLIK
                     EKYKSTVIVFPTSSSSISKSIMNINYLPYNKADLLNFGDEDDCDQLTQVLMSAEIKD"
BASE COUNT         4720 a         2549 c         2756 g         4716 t
ORIGIN      
        1 cagacttttt gcggctttca gaaagggttg gtgctaaata ccgacaaagt cgggagtaac
       61 cacttttcct tttttctttc gttcattttg caaacgttcg tgcatacgtt tataactatt
      121 ttctttagta tgccacatca gattagtaaa atggttattg cgtttgttcc aatccagatg
      181 gataacaaca gattcatccc cttcaatttt atcaacaaaa agctccgcaa caagtttatg
      241 aacaagaaaa tgcctggcac cttctttgca ttttaagcta actacttcaa aaccaccaat
      301 taaagaaggt tttaatattt taccatcttt actgttttgg caataacttt taacccggcc
      361 aaaattgctt atctcatatt ttgaatctgt gtcaggaata atagcccaca cttccccttc
      421 aatctcctgt ttccgtttat ttcgcgtcat aattatacat ttttgacttg gacaaatata
      481 tgaaaattca tgttcgaaag tataattatt tttcttttgt aagtaaaaga aaaacaataa
      541 aatacagcag ttatacgtct caaatattac ccttaagtat ttaaaaaatg cctaaaatta
      601 ggtatatttt ttacaccaaa ctttatctaa ccaattgtat atgaactatt tatttaagtt
      661 taaaaaggtt tacactaaac tgttataagt ttaaaaaaaa taaacggtta tgcattttgt
      721 ttttatctga aaatttttgc aaacggatag cactaataat acttctattt ttgttttact
      781 ttataaaagc gttaactttg attaaaaaac ctaacattac aatgcaaata atattgacta
      841 ttttttttat aactatattc ttttaccccc ttatcggtca agaagaggca attccgtttg
      901 atacaaaagg gaaaatttgg gtgattgata aaaaagtaat taaaactatt aaacttttta
      961 aagatgaaga ggtaatagaa gcccgccttt ttacaataaa tgacagcata tccgtatttg
     1021 agaagctaat aaaaaaagac gataaaacat ttcgagacaa acaaattctt tctaacagcg
     1081 agcttaattc acttcgagcc aaaatagaca gtatatcgct tatggttatt aaccaggaag
     1141 gtcgttcgtg gttgttggca tcttctacat ttttggggct cacgttgtat gggccttctg
     1201 ttccatctat tattaacagc actaacgaca aaatacaagt tagttcgtac atgctaactg
     1261 cagcttccgg tttctttttg ccctggtacc ttacccgcaa caggtctgtc tcgtatgccc
     1321 acctaaacct tactactttt gggctttcct ggggttttct tcatggtgct caaatatatt
     1381 gggccacttc aggctataat aaccttaata atgcattaac ttttggtaca atctgtggta
     1441 ttactgaagg atttattgga tacaatttgg tagataagtt taatattagt tatggacaag
     1501 cacaactaat gactttttat agtgagatag gcctttttat aggatttgca gctgcccaaa
     1561 gatttaacat tgatgcagaa gaaaaacctg gaggttttca ggggctttca ttgttaggct
     1621 cggctctggg gcttggtatt ggttaccgtc aagggcttga caaaagtatc actgccggag
     1681 atgcccagat tattggcatc agcgctctgt taagcgcata tcttacaatt cctccattat
     1741 ctgatattgc acctgatgac tcgcgcaata tttcaacaac cctttctctg gctggtattt
     1801 gtggggcatt tattggttct aaacttattc gaaacataga tttttcatat ggacaaagcc
     1861 tgatgattgg gctcggcacc attgcaggag gttttacggg tgctggtgta ggatatttaa
     1921 tagacaacaa tagctcgcta cctatataca taagcggcct tggggcactt acttcgtttg
     1981 gattgatgtt caattattac aaaaacaaat cggagacctc ttatgaaaag aacaacttta
     2041 ggataaacct atatccacaa aattacctgg tatggaaccg ggctaacctt caaccaattt
     2101 atttaacaaa ccttccatta gtaagtattt ctaaaacatt ttgattttaa gccataaatg
     2161 attgattttt tatctaaaat ttactttatt tgtttaaaac attaaagtat gaattatctg
     2221 gatgtactta tcatcatttt gttgattatt gcagctgttt cgggttttaa aaaaggattt
     2281 gtccaccaac ttttttcttt actggctatc atcttaggaa ttctgcttgc cgtaaagctc
     2341 aatacgtggc ttacgggatt aataacccct ggtatatttt cttcagtgca aacagccaaa
     2401 attgttgcct ttatttttgt gctaattgtt gtttctattt tggcacacct tatagcaaaa
     2461 attcttgaaa gtttttttaa tgatatagac ttaggattaa tcaacaggtt gaccgggctt
     2521 atttttggcg tattaaaaat gtcttttatc cttagtgcct ttatgatatt agtagaattt
     2581 tctattatca aaataaattt tcctaaaaaa gaaagctgcg acagctcatt tttgtaccct
     2641 aaagtgaaag ttattgcacc tgcagttttt ccttacctga aaaccattac agtagaaatg
     2701 tcaaaaactt ttcattaatt tcttaaaaaa attcctatgt tttgggataa aattgtagca
     2761 tggcttcatg caatacgtct gaaatatgta gaaaagaaag cttacgtgat atttgtatca
     2821 ataattatag gcatcttttc agcattggct gctgtaatgc ttaaaaatat tgtatattac
     2881 atcaaaatat tgctacaata cgactgggga atatcttatt tgtctatact gtactttatc
     2941 tatcctttag tgggtatatt tattgtgtat atttatttgc gggtttttca tgatggacat
     3001 atcaacaaag aaataggcta ccttatatac aagctatcgc gaaatgaaaa tgacctgcca
     3061 cggcgacaaa tatatgcaca ccttattaca agtgggacaa cagtaggttt tggtggttct
     3121 gtcgggcttg aagccccgat tgtactctcc gggtcttcta ttggttctgc catttctaaa
     3181 tatttcaggg taaacctgac agaccgcaat ttacttattg cttgtggcgc agctggagga
     3241 atatctgcta ttttcaatgc acctattgca gcaacaattt ttgcttttga agtacttttg
     3301 ccatccatgg ctgtttcagc atttgtacct ttgcttattt cttcagccac cgcatcaatt
     3361 ttctccaact tattatttgc cgaaaaacct tttgtactaa taacaaatgc ttgggaagtc
     3421 aatgacctga ctttttacat tgtttttggc gttttgtgtg gattgttggc ttcgtatatg
     3481 ataaaaatga gctattggat aggccgcatg atttttagaa atatcaaaag ccaggtttca
     3541 cggcttatta ttggctctat tctcctgggc agtatgattt ttatttttcc tgccctatat
     3601 ggcgaggggt acgacatggt taatgccctg tttgttaata atgtaaacct ggttattata
     3661 agccctttat ttcaaagttt gccaacaaat acctggataa ttcttattgc tattaccata
     3721 atcctgttga taaaagtaat tgctacttct ctgaccttgg gcgctggcgg caacgggggt
     3781 atttttgccc cttctctttt tacaggggca tttgccgggt atgcttttgc aaccatcatc
     3841 aacctcaccg gtgttgccca tgttaacgct atatgcttta tggcagtagg catggctggt
     3901 gtttttagcg gtgtcctcaa agcccctatg acagctattt tccttattgc agaagttaca
     3961 ggcgggtata gcctttttct cccattaatg attgtttctg cattatccta ttttattgca
     4021 agatacattg agccacattc ggtttatacc aaaatgcttg ctgaagataa aaaatgggat
     4081 cacgaaaaga ttgattctgt aatgcttcaa agactttcta taaaagattt gatagaaaca
     4141 aactttacat gcatcaccat agacgattcg ctctttagta ttcgcgaaaa agtatttagc
     4201 tcatcacaca aatttttccc tgtggtggac aaagaccttc atttgaccgg cattgtccat
     4261 ttcaatgata ttaaaaattt tttcctgaat aaacatgaag aagattttgg ttgggaaatg
     4321 cttatcaagc cagttaaaga caaaattgaa atcaatgatt ctgttgaaga ggcactgaaa
     4381 aaacttgaaa acgttggggc ttggaactta cctgttactg ataatggaat atacaaaggt
     4441 ttccttttca ggtcacgtat cctcgatagt tatagacaac atgttataca gcaacgcact
     4501 atgttttaag ctatttaaaa ttaaaaaaag cataacttta attttgtaac aaaatttgat
     4561 atgaggacag gtttattcca atttattgtc tgagatggga aaaattattc ttaattatca
     4621 cgattatttt aaaaaagatt tacgtttaat atttattata acggttattc tttcctgcat
     4681 tggtaacata gcagacatta atgttgtcat ggcccacagc aattatgcat tatatatcaa
     4741 ttttgccaat gtttttgtca accttatagc gctgttactg ttttttcggt ttaaagtacc
     4801 tgtcagaata tgccttggga ttgtaattta tgctgttatt atcaacctga tgggaagtca
     4861 gctttatgat tttccaaagt ccaattctgt aatgtatttg ctaagggcaa ccctgataat
     4921 agcccttgca gtgactgttg cagctatatg tatcagcaaa atgcactctt tcattttatc
     4981 cggcttgttt attgcttttt attcctatct ggctatttcg accaataaca aatttctgat
     5041 agacaacttt ttgattatct gcattatgtt tcttatttac aacgtggtta cttacctttt
     5101 tgtacgcaac atccacaatt ctatcatgca attgcaggaa caaaatgccg taatcacaga
     5161 acaaaaagag tcattagaag aagcctattg gaatcaagaa gagtctcgta ttttgctgaa
     5221 caaaaaaaat gaagagctga cgatagcaaa aaataaggcc gaagaaagcg acaggctcaa
     5281 gtctgcattt cttgccaaca tgagtcacga aatccgcacc ccgatgaacg gaatattggg
     5341 gtttgccaac ttattgaaaa aaccatcatt aagtggcgaa aaactgaaca aatatattag
     5401 cgccattgag ttaagcggca agcggatgct caatatcatt gacgatttga tagatatttc
     5461 aaaaattgaa gccggacaga tagacataaa agtcgaaaaa accagtatat atcaggttgt
     5521 aaatgagctt tgtgtgtttt tcaaaccgga agctgataac aaaaaaattg aactgcaaat
     5581 agccattgat agaaacgacc atgaatgttt tatcaaaact gataaaacaa aactgttaca
     5641 aatattgacc aacctcatta aaaatgcgat aaaatttacg catgaaggga gcattcagat
     5701 tggatacaaa ataaaaggac gagtagtaga attttttgtt aaagatacag gtattggcat
     5761 ccctgaatat atgcacgaaa aaatttttga acgctttctt aaagccgata atacattaat
     5821 caagccatat gaaggcgcag gcttaggatt agctatctca aaagcctatg taaatatgct
     5881 cggaggtgaa atctggctta cctctaaaga aaacgaaggc acaacattct ttttcacttt
     5941 accgttaata tcaggcaatg ctaataatgt tagcaacaat gttgttgatt tttatcctct
     6001 tgatcaggta gtaagcatca aatcaaataa aactatattg attgccgaag acgatgaaat
     6061 cagctacatg tacctctctg aatttcttga tggcagtggt attacaataa tcagggcaga
     6121 agatgggcaa aaagctgttg accttgtaca gcaaatgccc aacctggacc ttgttttgat
     6181 ggatattaaa ctgccacaaa tgaatgggca cgaagctctg agcctgatac gaaaaatacg
     6241 cccacatttg cccattattg cccaaacagc ttttgccatg caatctgaca ttgacaggta
     6301 tatatccgaa gggtttaatg attatctgac aaaacctata caaatagaaa acctgaacaa
     6361 tattttagca aaatacctac aataaaaaat atagttttta gtttaaacta tttgaatatt
     6421 aataacatgg tttcatagaa aaggtgtgct tttaatttaa gaaagccaaa ctaagattat
     6481 tgaaaggatt attttctata tttgtttcgc aaaaaatcta tcaattatgg caaaagtttc
     6541 tgaagagttt gactcaactg caatcaacct gcttggcgca ggtacaagta ttaacggtga
     6601 catcaattca aacggcgata tccgcatcaa tgggactgtt tctggcaata taacaaccaa
     6661 aggaaggtta attattgggg aatctggcaa aataaaagga gacatatctt gtcggaatgc
     6721 cgatattctt ggatatttcg aaggtaacat tcaggtaaca gagcttttat ctctcaaaag
     6781 ttcatcttct atcacgggcg agctgaatgt tgggcgttta gctatagaac ctgggtgcaa
     6841 attcaatggg acctgcaaaa tgaatgacaa tagccctgtt gctgcagaaa ataaagatgc
     6901 gaaatgataa caattggatg cgaaactatg tgaggtactc ctcactaatg ctccagatga
     6961 taatcataat tgttgttggt gtttatgggg gttatcgttt ggacttgtca ttttctgatg
     7021 gtaaacactg gtttctcatc ctattaacga ttgtttctac atttgtagcc ttgtatttat
     7081 ttatcaaaga ccttttgaaa aaaggataat ccagacatcc aatgcccaaa aaatatctct
     7141 tatcgtattg ttttaaactt atcgcgatga acctaacctt agggttatct tttgcaggtt
     7201 attattattt cctgcaccgc gaaagctcct cacaatactg gcctgcactg ctcgttatat
     7261 tttcggcagt aaattttgct ttccatctat ttctcatcca atcattttta aaaaggccag
     7321 ccgtattttt caggtctttt cttttgctta ctgtattaaa acttttaatt tacagtagtg
     7381 ttattattat ttttatattg cagattaaca aggggatggc agactttctg atatcttttt
     7441 taatcattta tttagctttc acaatttttg aagtttttga gctaattaaa atttcaaaaa
     7501 aaaatataat taattagaat tattcatacc tttgatgggc ttaaaaaggt ccgcaagtta
     7561 aacagtaaag tttaagatta tggctcggtc cattacattc gctaccatta tttcttttat
     7621 gctgctttca tttaatatgt atgggcagca tattactgct gatactgcta tttcgcatga
     7681 cagtgttgtc acagaaaaag cccacaatcc agaaggaggc tttaaaccag gggactttat
     7741 gcttgaacat atcctcgata gtcacgaatg gcacatcatt actgttggac atactcacgt
     7801 atctattccg cttccggtaa tcctttacag taaagtatcc ggcctgcatt ttttcatgtc
     7861 aagcaagttt catcatggta cagaaagcta taaaaacttt tatattgccg aagaaggccc
     7921 gaataaaggt aaaatagttg aaaaagtaca aagtgaacat ggcgaaaaag aaatcgtacc
     7981 attagattta tctatcacca aagttgtatt ttcgctttta ttgtcctgta ttcttttatt
     8041 ggtagtattt atcagcattg cacgaaaata tacacgcgaa ggcataaaag ctccaagtgg
     8101 attgcaatca gcccttgaac cgatgatttt gtttgttgta gatgatatag ccaaaccatc
     8161 ccttggcgag aaaaaatatg aaaagtttct gccatattta ttgaccatat tcttctttat
     8221 ctggttcaac aacatgttag gacttatccc tattttccct ggaggggcaa atgtcacggg
     8281 gaacattgca gttacaatgg tcttggcagc cttcactttt gtaattacta ccattaacgg
     8341 gaataaacat tattgggaag aaatttacaa tgcccccgga gttccctggt ggcttaagtt
     8401 tcccattcca ttaatgcctt tcattgaaat tttaggattg attataaagc cagttgtttt
     8461 gatggtccgt ctctttgcca acataacagc aggccactta atcatattgg catttgttag
     8521 tttgatattc atttttgccc agattagccc ggcattgggt tatggagtat ctgtgttatc
     8581 aactgcattt gcattattta tgagtttact ggaattactg gtagcgctca tccaggctta
     8641 tgtgtttaca ttgttgtcgg caatatactt tagcatggct actgccgaac atcattaatt
     8701 aataattaaa aatttaaacg tatgttaaca gctttgttga ttttacttga agctgcaggt
     8761 gtagcaaaac tcggtgcagg tgttggagca ggcttggctg ctgttggtgc tggtttaggc
     8821 attggcctta ttggtggcaa ctttatggaa gctatagccc gtcaacctga agcagttggc
     8881 gatcttcgtt caaacatgat tattgcagct gcgttggttg aaggagttgc tttcttcgct
     8941 attatcgttt gtttgcttat ccttttcttg taaaaagaaa aaataccgga acttgcgatt
     9001 ggcagttccg gttttattga aaaagttaaa aaacatcata aacagagaat tatggaactc
     9061 gttacgccca atgtcggaac cattttttgg atgctacttt tcttcagcct tgtattgctt
     9121 attttgaaaa aatttgcatg gaagcctatt ttaaattctc tcaaaaatcg tgagaagagc
     9181 attgaagatg cccttaagag tgccgaacat gccaaggaag aaatgcaaaa gctcaaacag
     9241 gacaatgaaa aaatccttgc tgatgccagg cgtgaacgtg actttttgat gaaagaagct
     9301 cgcgaaatga aagataaaat cattgctgac gcccaggaat tggctaaggt tgaagctaaa
     9361 aagcttatag atgctgcaaa acaaaacatt gataatgaaa agcgtaatgc tatcaacgaa
     9421 ataaaaatgc agatagctaa tctttctatc gaaattgctg aaaaaatcat tcgagaaaag
     9481 ttggatgaag aaaagcaaaa gcaaattgtt gaaaagatgc ttgatgaaat taacctcagt
     9541 taattatttg tgcaatgaac gaaagtatta tcgctgtcag atacgcaaaa gcgctttttc
     9601 aacttgctgt tgaaaaaaac aaagccgacg ttttaaagaa tgatatggaa actctttatc
     9661 actctattga agctgttagc gagctgaaag cattatttta caatcctgtt gttaaacccg
     9721 gaatgaagac aaacattatc cgtgaggttt tcggctcttt tgatgcagat actttgggtt
     9781 ttttaacatt attaattcaa aaccgcaggg aagaatatat tgaagaaatt gcactcaatt
     9841 ttatcaaacg atacaggaaa tataagggca ttgaaaaagt aatttttact actgccatta
     9901 agattgacgg agatattgaa agcaagataa aacaacaaat tggtaaatat ttaaaagctg
     9961 atgttgagtt tgaatcccag gttgatgaac gccttattgg agggtttagg ctacgccttg
    10021 gagataagca atacgatgcc agtatctcaa caaaattaga agatttaaag agaaaacttg
    10081 ttaaaacatc tatacattaa gcaatagaaa aaattcagaa tatggcaaac attaaaccat
    10141 cagagatttc cggaatactt aaaaaagcca ttgagggcgc tggcgctggt gtggcaatgg
    10201 aggaagtcgg tacagtatta caagttggag acggtatagc ccgtatttat gggctatcac
    10261 aagttcagag caatgagctt attgagttcg ccaatggcat gaaaggtatt gtacttaacc
    10321 ttgaagaaga taatgttgga gcagtattac ttggcccttc tacagagatt cgcgaggggg
    10381 acacagtaaa gcgcacaaaa cgcattgcgt caattaatgt tggagaaggg atgcttggcc
    10441 gtattattaa tactataggc gaaccacttg atggtaaagg cccaattacc ggcaagacct
    10501 acgaaatgcc gcttgaaagg aaagctcctg gcgttatata ccgtcaacct gtaaaagagc
    10561 cacttcaaac aggtattaaa gctattgatg ccatgatccc tattggacgt gggcaacgtg
    10621 agcttatcat tggcgaccgt cagacaggaa aaactgccat agcaatagat accattatca
    10681 atcaaaaaga attttttgat aaaggcaccc cggtatattg tatttatgta gctgttggtc
    10741 aaaaaggctc gactgttgcc aacattgcaa agactttgga agaacatggt gccatgcact
    10801 ataccactat agtagcggca acagctgccg atccggctgc tatgcaattc tatgcgccat
    10861 ttgccggttg tgctattggc gaatatttca gagatacagg acgtcctgca cttatagtat
    10921 atgatgatct ttcaaaacaa gcagtatcat atcgcgaggt gtcattgttg ctccgtcgcc
    10981 ctcccggacg tgaagcatat cccggagatg tattttatct gcactcacgt ttgcttgaac
    11041 gagcagcaaa gattattgaa tcagatgaaa ttgcagccca aatgaatgat ttacctgaga
    11101 ccttgaagcc tattgttaaa ggtggcggtt cacttactgc acttcctatc attgaaacac
    11161 aagctggtga cgtttcggca tatatcccta ccaatgtaat ttctattaca gatggacaga
    11221 tattccttga atccaacttg ttcaactctg gagtaaggcc tgctatcaac gtaggtattt
    11281 cggtatcacg tgtaggaggc aatgcccaga taaaatctat gaaaaaagtt gccggaacat
    11341 tgaaacttga ccaggcacaa tataaaaacc ttgaagcatt tgccaagttt ggctctgacc
    11401 ttgatgctgc aacattagca atattggaca agggggctaa gaacgttgaa attcttaaac
    11461 aagggttgca ttcgccactt actgtagaaa agcaaattgc cataatctac tgtgggacaa
    11521 atggtttgct taaagacatc cctctttcaa aagtaaaaga gtttgagttt gagtttttgc
    11581 aacatataga gatgaaaaat ccagaagttc tttcaaaact gagagatggc attattaatg
    11641 aagatatctc caagactatt gagaaacttg ctgctgaagt agttgataaa ttaaaaagct
    11701 aaaattacca gagttagttt taaagcaata tggccaattt aaaagaaata cgtacacgaa
    11761 tagcatctgt aacctcaaca aagcagatta ccagtgcgat gaagatggta tcagctgcaa
    11821 agcttaaaaa agcccaggat gcagtgttgc aactgcggcc ttatgcagat aagctgagta
    11881 atattttgaa agatgtaagc gatagttgtg ataatattac agaaaatgtt tacattaaac
    11941 caaggccggt aaaaaatgtg cttgttgttg ttattacttc aaacaggggg ctttgtggcg
    12001 ggttcaatgc caatgccgtt aagcttgtca tgaaccttgc acaaaatgaa tataataact
    12061 tatttaaaac taacaggttt aaaatatttt gtattggcaa aaaaggcaac gatatgttaa
    12121 agtcaaaaaa aatgcctatt taccaataca gagctgattt agtagataaa ccaacatatg
    12181 cccgtattga agatacagct aatgatttgc ttgaatggta taaatctggg gaatttgata
    12241 agataatttt gatctataac cgctttaaaa atgctgcagt acagatttta gccgaagaac
    12301 aatttatacc tgtattgtta gaaaaagaaa aagaaaaaaa acgcggcata ctttccgact
    12361 atatttttga accacagcaa gcagagcttg ttgacgaatt gataccatat actttaaaaa
    12421 tacgttttta taacgcaata cttgattcac ttgcatctga gcatggggca cgtatgacag
    12481 ctatgcacaa agccactgac aatgcctctg aattgattaa agagctaact ttgagctata
    12541 ataaagcacg tcaggcaacc attacaggtg aaatacttga aattgttgga ggtgcagagg
    12601 ccctcaaagg ataattttta tggctttaaa gcacttatta tgtacacata ttttatttcg
    12661 cctaagtttg gagatattga cgggttgggg catgtaaata atacagtaat acccagttgg
    12721 tttgaacaag cacgaaacta tctttataaa ttatttaacc cggagttaga tttaaaaacc
    12781 tggaacctta ttttagccag gtttgaagtt gattttgttt cgcagatgta ctactcaaaa
    12841 gatgttgaaa tcaggacttt tgtatcaaaa ataggccgtt cttcatttga agtatatcag
    12901 gaggcatggc aaagcgggca tatttgtgca aaagggaaaa ccgtactggt gcattttgat
    12961 tttatacagc aaaaatctgc tgccattcct gaagatatac gcgaaaaatt atccgaacat
    13021 cttattgcag ccattgatgc tactgctaat taaagaatag ttgtaaactc tcattctttt
    13081 cttgggctaa ttattatctt tggcacttga aaattttaga cgatataact atggactttt
    13141 ttgaagagct caagtggaga gggatgatac atgattacac tcccggtttg gaagcacaat
    13201 tgcaacgcga aatgaccact gcttatgttg gtatagaccc cacagcagat tctttacata
    13261 ttgggcatct tgtttctata atgctgctta aacatttaca ggtagctggt cataaaccta
    13321 ttgccttagt tggtggggcc acaggaatga taggggatcc ttcagggaaa tcagaagaaa
    13381 ggaacctttt gtctttagaa acccttcgtc ataaccagga atgtatcaaa aaacaattat
    13441 cccgtttttt aaattttgaa acacaagaaa aaaactgtgc tattcttgtc aataattatg
    13501 actggatgtc cacttttagt ttccttgatt ttatacgtga tgtaggaaag cacattactg
    13561 taaactacat gatggccaaa gattctgtaa aaaaacgcat ttcgggcgaa gaccgcgaag
    13621 ggatgtcgtt tacagaattt acctatcagc ttgttcaggg ctacgatttt ttgtatttat
    13681 ataaaaatta cggttgtaag cttcagatgg gaggttcaga ccaatggggc aatatcacta
    13741 cagggacaga aatgatacgc cgtattgaag gcggggaagc atttgccctt acctgccctt
    13801 tgattactaa agctgacgga ggaaaatttg ggaagaccga aaaaggcaat gtttggttat
    13861 cttcagcata tacttcacca taccagtttt atcagttttg gatgaatgta tcagatgctg
    13921 atgcagaaaa gtatatcaaa atatttacca tgctgccacc tgcagaaatt gaagatatta
    13981 ttgaaaaaca taaagttgag cctcatttac gtttgttgca gaaagcttta gccaaagaag
    14041 taacagtaat ggtgcattcg caagctgaat atgagtctgc tgttgaggca tcagaaatat
    14101 tgtttggcaa aggaactgca gaaacattag ccaatttaag tgaaaatgat tttcttgcaa
    14161 tatttgacgg agttccacgt tttgaaatta cccgcgaggt attaaaaagc aatataggta
    14221 tccttgattt attggcatct tcaacagccg ttttccctag taaaggagaa gctaaacgat
    14281 tgattgaagg aggaggtttg agtatcaata aaaataaagt caatagtgct aatgatactg
    14341 taaatgagag ctttttgctt aatgaaaaat atttgttagt tcaaaaaggg aaaaagaatt
    14401 ataacctgat cattgttaaa taattaacct tataagtatg acgaaaaaag acaaatttga
    14461 atttgatgct accagcataa ttaaattcac tttaaagaac ataaagttgc taatagcagt
    14521 ttcaattgtt gcttttattg tatctgctgt agtttctttt ttaattaaag aaaaatacaa
    14581 atctactgtt attgtatttc cgacatcatc atcatctatt tcaaaatcaa taatgaacat
    14641 taattattta ccatataaca aagctgattt gttaaatttt ggtgacgaag atgactgtga
    14701 ccaattgacc caagtgttga tgtctgctga aataaaagac a
//
LOCUS       DAOSPF010000070        14643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4143_length_14643_cov_6.832534, whole genome shotgun sequence.
ACCESSION   DAOSPF010000070 DAOSPF010000000
VERSION     DAOSPF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4143_length_14643_cov_6.832534"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1356)
                     /gene="hydF"
                     /locus_tag="PK252_11985"
     CDS             complement(136..1356)
                     /gene="hydF"
                     /locus_tag="PK252_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOT15471.1"
                     /translation="MAKENKPHIGIYGRRNNGKSSWINKLAGQDVAIVSDIAGTTTDP
                     VKKSMEIHGAGPVIMIDTAGIDDVGELGQKRIAKTLQSINHIDLAILVVTNNEFGEPE
                     KKLINQFIEKEVPYFILVNKCDVDKPHESFIQMIKKETNAEVLTSSINNQADEDMVLS
                     LIKKLLPENAWKKESLFGDLVSYGDVVLLIMPIDNEAPEGRLILPQVQAIRDALDNDC
                     VAIVVKEREADAFLRNSGIKPKLAITDSSIFIKADAIVPKDVPLTGFSVLLARHKGPF
                     DTYLNGTPYISKLQDGDKVLILESCTHQVSCDDIGRVKIPRWLSQFTGRKLQFEVASG
                     LSTLSSDLKQYALVIQCGGCMITRRQIYSRLKPAIDEGVPVTNYGMAIAYIQGIYNRA
                     IQPFVKGHIDLSYL"
     gene            complement(1544..1753)
                     /locus_tag="PK252_11990"
     CDS             complement(1544..1753)
                     /locus_tag="PK252_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HOT15472.1"
                     /translation="MKFKHGTYRHFKGHMYETIGIAKHSETLEDMVIYVNQEDEKEIW
                     VRPLSMFTDEVTRDGKTFKRFEFLG"
     gene            complement(1772..4021)
                     /locus_tag="PK252_11995"
     CDS             complement(1772..4021)
                     /locus_tag="PK252_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT15473.1"
                     /translation="MSNIIIRNARVHNLKNVSIEIPKNKIVVFTGVSGSGKSSLVFDT
                     LYTEAQRQLIETFSSFARQRLPKLSRPDVDEIRNISTAIVIDQKRMGRNLRSTVGTAT
                     ELSTYLRMLFSRFAEPFIGPSFFFSFNHPEGMCPYCKGLGKKIAVDINLLIDKSLSLR
                     EGAITHPDMKVGGWHWRELVACGLFDPDKKLGEYSKEEWNNLLFAESIPVERKHGAGT
                     YLKNHVGIVRKLEGYYVEKSEDEVPETRKDAYQRYFIYSECEMCKGTRINDRARSSLL
                     LGKNYAELSNIELVDLDAFLANINIEAAQVIISKMRSILGHLINIGVHYLSLDRPVST
                     LSGGESQRVKMARQLDCDLVEMMYILDEPSIGLHPKDNAHLINMLRQLRDKGNSVFVV
                     EHDPDIMRCADWIVDVGPRSGVRGGQIVFQGSYESLKSADTLTAQYINMPPHNGYTRK
                     PWTESYAIKNATLHNLKNVSVEIPKGVLTCITGVAGSGKSSLINGVFLNNNPDAIVID
                     QQAVTRSSRSTPATYIGFFDLVRKEIANATGANASLFSFNSDGACPKCKGMGYISLEM
                     SFMDDVNIECDECNGQRFKDEVLAIKYKGKNIFEILNMPAEEAVDFFENNETRKRLKV
                     LCETGLEYLDIGQSLSSLSGGEAQRIKIASELHKKGNLYIMDEPTTGLHMADIDRLVS
                     IIKKLVKNNNTVVVIEHNLDLIKHADWVIDMGPDGGKNGGCVLFEGTPENLVECKNSY
                     TGQYLKNVL"
     gene            complement(4027..4395)
                     /locus_tag="PK252_12000"
     CDS             complement(4027..4395)
                     /locus_tag="PK252_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOT15474.1"
                     /translation="MKQVIIKDKTFEFAVQIVELYKRLHDTKKEYVLGKQLLRSGTSI
                     GANTREALNAESKADFIHKLAIAQKECDETIYWLELLKESNFISSAEFDSYNSKATEI
                     LKILRSIILTTKQNLITHNS"
     gene            complement(4438..5301)
                     /locus_tag="PK252_12005"
     CDS             complement(4438..5301)
                     /locus_tag="PK252_12005"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT15475.1"
                     /translation="MTNKEVILKSIRFIEENLKAEITVLDCAQNVCYSLYHYIRLFQS
                     VTGYSPKNYIQQRRLSEAAQELMNADKKIADLAFEYQFNSHEAFTRAFTKLFGFKPTE
                     IRNKQALTVYPLVKPITADFIFQSEKARNQPPEKVELEERMLAGIAFFVPDDSDGAII
                     PEKWAEFLKEVNDIPNRVVPERYYQIQYWSNVQEIGGLYFFVGLEISSMDNLKPEYVL
                     KTIPRGKYLRFIHCGLSNKVGYTYKYIYNQYLPDSDHVLSLPFNFEFYGEKYKGPYNP
                     ESESEIYIPIE"
     gene            complement(5501..5977)
                     /locus_tag="PK252_12010"
     CDS             complement(5501..5977)
                     /locus_tag="PK252_12010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HOT15476.1"
                     /translation="METTISITPIGKVLKENGMFIIELEKEYLPALIGIEGFSHLQII
                     WWGHLLDKPENRKVLITNKPYKTGPEVLGIYATHSPVRPNPILITTIAVASFDKVKGR
                     IATGYIDAEPGTPILDIKPYHKNERIRDCRVPDWCSHWPDWNEDSATFDWQKEFNF"
     gene            complement(6298..6924)
                     /locus_tag="PK252_12015"
     CDS             complement(6298..6924)
                     /locus_tag="PK252_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOT15477.1"
                     /translation="MSIQFFLTYLSIVTIATITPGPSMLMAINHGVNHGLRKTFVSGL
                     GNMIGNILMAIVSIIGLGALLIASGVIFNIIKWCGVAYLIYLGIMTFFASTSSKEEIS
                     EKPYKEKSTYRLFSEGFIIAIGNPKGILFFTALFPQFISTDSASISGFSFLIIPLAIV
                     AITCYMLYAAVGTRISKLFAFATFRKTYNRIVGSIFIGTGIALAFSRK"
     gene            complement(6943..7752)
                     /locus_tag="PK252_12020"
     CDS             complement(6943..7752)
                     /locus_tag="PK252_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT15478.1"
                     /translation="MHFQFIKPSGILAKYVRHYWILEAEASEGEVCERVVPTGNIELL
                     FHYRKPFVMKHCDNTTTVQSLSMLGGMSNAYSDVYTQGEAGLLAVKFYPHGACNFFRF
                     PLVEVENIGINLQEVFNKEIKRIEEQVTAAKDLQNRIAIIERFLISKLCPVKESEYLL
                     LNQSIQYINSTGGQITARELCSKLATTPRSLERKFATFMGKSPKQFIRIVRFQEVLRR
                     LSLGDKTYLTEYAYNNGYFDQSHFIKDFKSLSGYTPKEFVALGSCHSDYFG"
     gene            7868..8626
                     /locus_tag="PK252_12025"
     CDS             7868..8626
                     /locus_tag="PK252_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT15479.1"
                     /translation="MKKIALVTGVTSGIGLATARILAKNGYNLIITGRRKELLEKVKT
                     EITANTDAQVLDLNFDLRKRKDVVDAIASLPEKWKAIDVLVNNAGLASGLDPIQSGDI
                     EDWEKMIDTNVKGLLYITREVAPLMVARNNGHIINISSIAGHEVYPNGNVYCATKHAV
                     EALNKAMRMDMVKHNIKVTSIAPGMVETEFSIVRFHGDTNRAKKFYDGLTPLNADDIA
                     DAILYVVTRPAHVCINEMLIMPTAQASATVVNRS"
     gene            complement(8682..10388)
                     /locus_tag="PK252_12030"
     CDS             complement(8682..10388)
                     /locus_tag="PK252_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOT15480.1"
                     /translation="MANFYTDTPELKFHLNHPLMRRIVELKERNYTQKDKFDYAPHDY
                     EDAIDSFEKVLEIVGEICGDIIAPNAESVDHDGPQLVDGHVQYAPGTVVNLDALNKAG
                     LMGMSLPRRFGGLNFPIVPYIMAADMVSRADCGFVNIWGLQDCAETINEFATEEQKMR
                     YLSRVCKGETMAMDLTEPDAGSDLQAVMLKATYDEKQGTWLLNGVKRFITNGDGHISL
                     VLARSEEGTTDGRGLSMFIYDKNDGGVTVRRIENKMGIKGSPTCELVFKNAKAELCGQ
                     RKLGLIKYVMALMNGARLGIAAQSVGVCEAAYREALAYAKDRQQFGKAIIEFPAIYEM
                     LSLMKAKLDAARTMLYETARFVDVYKTLMHISEERELTKEEKEEMKKFQKWADMFTPL
                     AKGLGSEFANQNAYDAIQVHGGSGFMKDYACERIYRDARITSIYEGTTQLQVVAAIRF
                     VTSGNYLTKIREYEAEQIKPELETYRRILVAMTDEYEAAVKKVVEVNNPEYIDFHARR
                     LVEMAGYIIMGYLLLSDTNRDHNYWKTTEIFVKWARSQNAQRADFIKNSSVNDLGKFK
                     ME"
     gene            complement(10486..11505)
                     /locus_tag="PK252_12035"
     CDS             complement(10486..11505)
                     /locus_tag="PK252_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT15481.1"
                     /translation="MNNIVVYCEIEEGKVADVSLELITKGRKLAQDLKCKLEALAIGY
                     KLDSIGSQVFPYGVDTLYVVDDKRLEHYQTLPHTSICVKLFEEQKPQICLMGATTTGR
                     DLGPRVSSALKSGLTADCTNLEIGPHTETKTGKEYKDLLYQIRPAFGGNIIATIVNPD
                     CRPQMATVREGVMKKEIFNANHKGEVVKLDADKYVSASDFAISIVERHMEKSKVNIKN
                     SSIIVAGGYGVGSKENFALLYELAAVIGAEVGASRAAVDAGYAEHERQIGQTGVTVRP
                     KLYIACGISGQIQHIAGMQESSMIISVNNDPNAPINKIADYVITGNIEDVLPKMIKYY
                     KQNSK"
     gene            complement(11582..12454)
                     /locus_tag="PK252_12040"
     CDS             complement(11582..12454)
                     /locus_tag="PK252_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT15482.1"
                     /translation="MGLKIIVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRLKDQFPGTHITLLTMGPVRAAEVLREGLFRGADDGILLTDRAFAGADTLAT
                     SYAIACAIRKVKDYDLIICGRQAIDGDTAQVGPQVAEKLGIPQITYAEEVVSVKNNVI
                     KIKRRLERGIEVVEGKLPLVITVNGSAAECRPRNAKLLMKYKHSSTMSERAEKSEDYL
                     AMINDKPYLNIKEWSVNDIECDREQLGLSGSPTKVKKIENIVFQAKESKRLTDGDNDI
                     NQLMKELIENHTIG"
     gene            12847..13557
                     /locus_tag="PK252_12045"
     CDS             12847..13557
                     /locus_tag="PK252_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15483.1"
                     /translation="MSLDTIIEFCNAKICQQDHLVMSNVNFKVDKGEFVYLIGKVGSG
                     KSSLIKTINAELPLTEGTGFVAGFELDKLKPRKIPYLRRKLGVVFQDFQLLTDRTVIK
                     NLEFVLKATGWKDKKEIEKRIADVLEKTGLTTKGYKMPHQLSGGEQQRVSIARALLND
                     PEIILADEPTGNLDPDTSAGIMNLLFEISHNGRAVIMATHNYTFLKNFPSRIVKCENG
                     TLVEKAQSEEIDFETLMN"
     gene            13687..13998
                     /locus_tag="PK252_12050"
     CDS             13687..13998
                     /locus_tag="PK252_12050"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT15484.1"
                     /translation="MSNKRMDRDEIIKYLTGQKEYFNHRYNIAQIGLFGSFARNTQTK
                     DSDIDIVYTMAQNKKLSYYQLFEIIETLESHFKRNVELVNDKYMEPIIKHDAQKDIIY
                     V"
     gene            complement(14440..>14643)
                     /locus_tag="PK252_12055"
     CDS             complement(14440..>14643)
                     /locus_tag="PK252_12055"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT15485.1"
                     /translation="SLVGILFTVVLAPLLLKIYGMKGAAVATSISYLASTIYLAYYFY
                     KVTEFKLVDFKVDLKELKALFGK"
BASE COUNT         4375 a         3012 c         2736 g         4520 t
ORIGIN      
        1 acatctgaaa ggatagaagt tctcaaaatt ttacattcac attcaagtat tactaaaatc
       61 agggggaccc gaagtacaaa actattaatt cactaaactc tctttcccag aatttattac
      121 tgacagactt tgactttaca aatacgacaa atcaatgtgt ccttttacaa atggctgaat
      181 agccctgttg tagatacctt gtatgtaggc aatggccatc ccgtagtttg taaccggaac
      241 accttcatca atggcaggtt ttagcctgct gtagatttgc ctgcgtgtaa tcatacagcc
      301 cccacattgt ataacaagtg catattgctt caaatcagat gacaacgtac ttaagcctga
      361 agcaacctca aattggagct tacgtccggt aaattgacta agccagcggg gaatcttaac
      421 acggcctatg tcatcacatg aaacctggtg tgtgcaagat tcaaggataa gtactttatc
      481 tccatcctga agtttcgata tataaggggt tccattaaga taagtatcaa aaggcccttt
      541 atgacgggct aatagcacac taaaccctgt taaaggcaca tctttaggaa caatggcatc
      601 tgctttgata aatatagagc tgtctgtaat ggctaactta ggtttgattc ctgaattgcg
      661 caaaaatgca tcagcttcac gttcttttac cactattgcc acacagtcat tgtccaacgc
      721 atcacgaatg gcctgaacct gaggcaatat aagtcttcct tccggagcct cgttgtcaat
      781 aggcataatc agcaacacta catcaccgta gctgaccaaa tcgccaaaca aagattcttt
      841 tttccaggca ttttcaggca atagtttctt aatcagcgat aacaccatgt cttcatctgc
      901 ctgattattg atgctggaag taagcacctc tgcattagtc tccttcttta tcatttggat
      961 aaatgattca tggggtttat caacatcaca tttgttaacc agaataaaat aaggtacttc
     1021 cttctctata aattgattaa taagcttttt ttcgggttct ccaaactcat tgtttgtaac
     1081 aacaaggatg gccaggtcga tatggttgat ggattgtagt gttttggcaa tgcgcttctg
     1141 ccccagctca ccaacatcgt caataccggc agtatcaatc atgataacag gcccggcgcc
     1201 atgaatctcc attgatttct ttaccgggtc agtggtggta ccagcaatat cagacacaat
     1261 ggctacgtct tgtccggcaa gcttgtttat ccatgagctt ttgccattat tacgccttcc
     1321 gtaaatgcct atatgtggtt tgttttcttt tgccatagct ataaaacatt taatatacca
     1381 aactgtttaa tatacaaaag tttctttgat tacttctttt acccgataaa tttcagttcc
     1441 agtaaggtta tcagtttctt atatttgttt tatccgaaca aaattgattt tcaatggtca
     1501 aatgattaaa acactctgta actctgtttc ttcggagtaa attttatccc aaaaactcaa
     1561 agcgtttaaa agtcttgcca tctctggtta cttcatcggt aaacatagac agcgggcgca
     1621 cccatatttc tttttcatct tcctggttaa cataaatgac catatcttct aaggtttcag
     1681 aatgcttagc aataccaatg gtttcgtaca tgtgcccttt aaaatgcctg taagtgccgt
     1741 gtttgaattt catatcgttt tttttacaaa gctaaagtac attttttaaa tattgaccag
     1801 tataagaatt tttgcattca acaaggtttt ccggagtgcc ttcaaaaagc acacagccac
     1861 cattcttccc tccatctggc cccatgtcaa tcacccagtc ggcatgcttt atcaaatcaa
     1921 ggttgtgctc aataaccacc acagtattgt tgtttttcac caattttttg ataatagaaa
     1981 caagcctgtc gatgtcggcc atgtgtagcc cggtggttgg ttcgtccatg atataaaggt
     2041 tgcctttttt atgcaattca gaagctattt ttatgcgttg agcctcgccg ccagacaaac
     2101 tgctcaacga ttgtccgatg tcgagatatt cgaggcctgt ttcgcaaagc acttttaagc
     2161 gtttgcgggt ttcgttgttt tcaaaaaagt caaccgcctc ttctgcaggc atgttcagta
     2221 tttcaaaaat gttttttccc ttgtatttaa tggcaagtac ttcatctttg aagcgttgtc
     2281 cgttgcattc gtcacattcg atatttacat cgtccataaa gctcatttcg agagatatgt
     2341 agcccatacc cttgcattta ggacaagctc catcagagtt aaagctaaac aggctggcat
     2401 tagcgccggt agcattggca atctcttttc gtaccaagtc gaaaaaccca atataagtgg
     2461 ccggtgtact acgtgatgac cgtgtaacag cctgctggtc tataactatg gcatcgggat
     2521 tattgttgag gaatacccca ttgataaggc tgcttttgcc cgagcctgca accccggtaa
     2581 tgcaggtcaa aacacccttt ggtatctcaa ccgatacatt tttcaggtta tgtaaggtgg
     2641 cgttttttat agcataagat tctgtccagg gttttcgggt atatccatta tgagggggca
     2701 tattgatata ctgagcagta agcgtatcag cgcttttcaa agattcataa gaaccttgaa
     2761 aaactatctg gcctccacga acaccagaac ggggtccaac gtcaacaatc cagtcagcac
     2821 agcgcataat atctgggtcg tgttcgacaa caaatacaga gttgccttta tctctcagtt
     2881 gcctgagcat attaatcagg tgggcattgt ccttgggatg taagccgatg cttggctcat
     2941 cgagaatgta catcatttct acaaggtcgc aatcgagctg ccgtgccatc tttacccgtt
     3001 ggctttcgcc acccgacagg gttgatacag gcctgtcgag cgaaagataa tgaacgccta
     3061 tatttatcag gtgccccaat atcgaacgca tcttggatat aatgacctga gcggcttcta
     3121 tgttgatatt tgcaagaaaa gcatctaaat cgacaagttc gatattgctc aactcggcat
     3181 agttctttcc aagaagaaga gaagatcgtg cgcggtcgtt aatccttgtc cctttacaca
     3241 tttcgcattc cgagtagatg aaataccttt gatacgcatc tttgcgggtt tcgggcactt
     3301 catcttcgct tttttcaaca taataccctt cgagctttct gacaataccc acatggtttt
     3361 tcaaataagt acctgcccca tgcttgcgtt caaccgggat agactctgca aacagcaggt
     3421 tgttccattc ttctttgctg tattcgccca gttttttatc ggggtcaaac agtccgcaag
     3481 ccacaagttc gcgccagtgc caaccgccaa ctttcatgtc ggggtgtgta atagctcctt
     3541 cgcgcaaact cagcgattta tctatcagca agtttatatc cactgctatt ttcttgccca
     3601 aacctttgca atatgggcac ataccttcgg gatggttgaa tgaaaagaaa aatgacggcc
     3661 caataaaggg ctctgcaaaa cgtgaaaaca gcattcgcag gtatgtacta agttctgtag
     3721 ctgttccgac tgtacttcgc aggtttcgtc ccatgcgctt ctggtcaata actatagcgg
     3781 tagatatgtt gcggatttcg tccacatcag gtcgcgaaag ttttggcaat cgctgacgag
     3841 caaaggacga aaatgtctca atcagttgtc gctgggcttc ggtgtataat gtatcgaaaa
     3901 caagcgatga cttgccagac ccggacaccc cggtgaatac aactatcttg ttctttggta
     3961 tttctatgga tacgttttta aggttgtgaa cgcgtgcgtt tcgaatgatg atattagaca
     4021 taaaagttat gaattatgag ttatgaggtt ttgtttggtg gtaagtatta tggaacggag
     4081 aatttttaat atttctgtcg cttttgaatt ataagaatca aattcagctg aggaaataaa
     4141 attactttct tttaatagtt caagccagta aatagtttcg tcgcattcct tctgagcaat
     4201 tgccagtttg tgaatgaaat cagccttact ttctgcattt aaagcttctc tggtattagc
     4261 tcctatactt gtcccagaac gcagaagctg tttacctaaa acatattctt ttttagtatc
     4321 gtgaagtctt ttatataatt ctactatctg aacagcaaat tcaaacgttt tatcttttat
     4381 aatcacttgc ttcattgttt attgtatttt attaactcat aacttttaac tcaagattca
     4441 ttcaattggg atataaattt cactttctga ttcaggatta taagggcctt tgtatttctc
     4501 gccataaaac tcgaaattaa aaggaaggct taatacatgg tctgagtcag gtaaatattg
     4561 gttgtagatg tatttgtaag tgtacccgac cttgtttgac agtccgcagt ggataaagcg
     4621 aagatacttc cctcgaggta ttgttttcag cacatactcg ggtttaaggt tgtccattga
     4681 cgatatttcg agaccaacaa aaaagtataa acctcctatt tcctgtacat ttgaccaata
     4741 ttgtatttga taataacgct cagggacaac acggttggga atatcattta cttctttcag
     4801 gaattcagcc catttttcag ggattatagc accatcagag tcgtccggca caaaaaaggc
     4861 aatgcctgcc agcatccttt cttcaagctc aactttctcc ggaggctggt tcctggcttt
     4921 ctctgattga aatataaaat ctgcagtaat gggtttcacc agtggataaa ctgtaagggc
     4981 ctgtttattg cggatttcgg ttggcttaaa cccaaatagc ttggtaaatg cacgggtgaa
     5041 agcttcgtgg ctgttgaact gatattcgaa agccaggtct gcgatttttt tatcagcatt
     5101 catcagttcc tgggctgctt cgcttaaacg tcgctgctga atgtagttct tgggcgagta
     5161 ccctgtaaca ctctggaata gccttatata atggtataac gaatagcata cgttctgagc
     5221 gcaatctaat acagtgattt ccgcttttaa attttcttca atgaacctta tcgattttaa
     5281 aattacttct ttatttgtca tagtttattt tgctgtgagt atagtaaacg aacaatattc
     5341 tgtatatact tcaaaaccaa acacttcaat ggtaataaag tagcaataag tattgtttcc
     5401 caaattacaa tagaacttga ttacagtcat attttttgtt aaaaaagttg gtttaggaag
     5461 ccagggcatc tgaaaagacc tgatacccga acttaattaa ttaaaagttg aattcttttt
     5521 gccagtcaaa tgttgcagaa tcttcattcc aatcaggcca atggctgcac caatctggaa
     5581 cacgacaatc ccttatcctt tcgttcttat ggtatggctt aatgtctaaa atcggagtcc
     5641 ccggctcggc atcgatatac ccggttgcta tgcgaccttt tactttatca aagcttgcta
     5701 cagcaatggt ggtgatgagt atcgggttag gcctcacagg cgaatgggtt gcataaatac
     5761 ccaggacttc agggcctgtt ttatatggct tattggtgat aagtactttt cggttctcag
     5821 gtttgtcaag caaatgcccc caccaaataa tttgcagatg gctgaatcct tcaatcccta
     5881 tgagggctgg caggtattct ttctccagtt caataatgaa catgccgttt tctttcaaaa
     5941 cttttccaat aggcgtaatg ctgatagttg tttccatgtt ttaaaaaatt taagattaac
     6001 aatttgcatt accggtaaat gcatggcaaa aataggaacc agtcattgaa ccttcttgac
     6061 gtttattgct aatgtgggga gcaatacttg actgaaattg ctaatttgct aaacccttat
     6121 caatctttga cttgaaacaa cagtataact gccacagttc tattagaagt gaacctctcc
     6181 ggcacttttt ttgcaaattt ctgattacaa tttcgttttc catgttttgt acttttgaat
     6241 tcgggtacaa aactatttta tggcagggtg ccaaaattgt aaaaaaacga caaaagccta
     6301 tttccgggaa aaagctaatg caatacctgt cccgataaat atagagccta ctatacggtt
     6361 gtatgttttg cggaatgtcg cgaaagcaaa gagtttactg atacgtgtac caacagcagc
     6421 gtacagcatg tagcatgtga tagctacaat ggccaaaggt atgattagga aactaaaacc
     6481 cgaaatagac gctgaatcag tgctgataaa ctgtggaaac aatgcagtaa agaacagaat
     6541 gcctttaggg tttcctatag caataataaa accttcagaa aataacctgt aagtactttt
     6601 ctctttatat ggcttttccg aaatttcttc tttgcttgac gttgaagcaa aaaaggtcat
     6661 tatacccagg taaataaggt atgccacgcc acaccatttg ataatattaa atattacgcc
     6721 agatgcaata agcaatgccc ctaaaccaat aatagaaact atagccatga gtatattccc
     6781 tatcatattg cccaggccac ttacaaatgt tttgcgcaat ccatggttta caccgtgatt
     6841 aatagccatg agcatgctcg gccccggagt gatagtagca atagttacta tagaaagata
     6901 ggtaagaaaa aactggatgg acatgggttt ttgttttaat aattatccaa aataatcaga
     6961 atgacaacta cccaaagcta caaattcttt gggagtatat cccgacagcg atttaaaatc
     7021 tttgataaag tgcgactggt caaaataccc gttattataa gcatattctg taaggtatgt
     7081 cttatctccc aggctcaaac gcctcaatac ctcttggaaa cggacaatgc gaataaattg
     7141 ttttggcgat ttgcccataa atgttgcaaa cttacgctcc aggcttcggg gggtagtagc
     7201 cagtttactg cacagctcac gggccgtaat ctgtcccccg gtactattga tgtattgaat
     7261 gctttgattg agaagcagat attctgactc tttaaccggg cagagcttgc taatcaggaa
     7321 gcgctcgata atggcaatcc tgttttgaag gtcttttgct gctgtaacct gttcttctat
     7381 tcgttttatt tctttgttaa agacctcttg caggttaatt ccaatatttt cgacctctac
     7441 aagaggaaac ctaaagaaat tgcaagcccc atgaggataa aatttaacag ccaaaagccc
     7501 agcctcgcct tgcgtataaa catcagaata ggcattgctc attcccccca gcatggataa
     7561 agactggacg gtggtggtat tgtcgcaatg cttcatcaca aaaggctttc ggtaatgaaa
     7621 cagcaactct atattgccag taggcacaac ccgctcgcat acctcgcctt ccgaagcttc
     7681 tgcctcaaga atccagtagt gccttacgta ttttgcaagt attccggatg gttttataaa
     7741 ctgaaagtgc atgaaggggt gattttgaat gcaaagctaa agtatttttg tacaaattat
     7801 taaaagaaag ctggcactat ttttgtcttt tcccagtatc tttgaaataa ttttaaacat
     7861 caggcttatg aaaaaaattg cacttgttac aggtgttaca tctggtattg gattggcaac
     7921 cgcccgtatt ttggcaaaaa atggttataa cctaattata accggaagaa gaaaagagct
     7981 tttagaaaag gtaaaaacgg aaatcactgc taatactgat gctcaagtgc ttgatttgaa
     8041 ttttgactta cgtaaacgca aggatgtagt tgatgccatt gcttcattgc ccgaaaaatg
     8101 gaaagctata gacgtactgg ttaacaatgc cggcttagct tctggccttg acccaataca
     8161 atctggcgat attgaagatt gggaaaagat gattgacacc aatgttaagg ggttgctgta
     8221 tataacccgt gaagttgccc cattgatggt tgcccggaac aacgggcata ttataaacat
     8281 tagctctatt gccggacatg aggtatatcc taacggaaac gtttactgtg ctacgaaaca
     8341 tgctgttgaa gctttgaata aggcaatgcg tatggatatg gtgaagcaca acatcaaggt
     8401 aacctctata gccccgggga tggtagaaac ggagttctct attgtccgtt ttcatggcga
     8461 tacaaatagg gctaaaaagt tctatgacgg attgaccccg cttaatgccg atgatattgc
     8521 tgatgctata ttgtacgtgg ttacgcgccc ggcacatgtt tgtatcaacg aaatgctgat
     8581 tatgcctaca gctcaggcta gtgccacagt ggtaaacaga agttagttat aaaaaaagcg
     8641 gaacctctcg attccgcttt ttgttatggt aataaatccg attattccat tttaaatttg
     8701 cccaaatcgt taacgcttga gtttttgata aagtcagcac gttgggcatt ttgcgaacga
     8761 gcccacttaa caaaaatctc ggttgtcttc caatagttgt ggtcacggtt ggtatctgac
     8821 aacaataaat atcccatgat gatataacct gccatctcta ccaaacgacg tgcatgaaaa
     8881 tcaatatatt caggattgtt cacttctaca actttcttca cagcagcttc gtactcatcg
     8941 gtcattgcta ctaaaatacg gcgatatgtt tcaagctctg gtttaatctg ttctgcttca
     9001 tactctctga tttttgtcaa ataattacca gaagtaacaa agcgaatagc agcaactacc
     9061 tgaagttgtg tagtaccttc gtaaattgaa gtaatacgag catcgcggta aatacgttca
     9121 caggcataat ctttcataaa gcctgacccc ccatgaacct ggatggcatc gtaagcattt
     9181 tggttagcaa attccgaacc caaacctttg gcaagagggg taaacatgtc agcccatttc
     9241 tggaacttct tcatttcttc tttttcttct ttagtcagct cacgttcttc tgaaatgtgc
     9301 atcaatgttt tgtaaacatc aacaaaacgg gctgtttcgt acaacatagt acgcgcagca
     9361 tccaattttg ctttcattaa agaaagcatc tcatatatag ccggaaattc aataatagct
     9421 ttaccaaact gttgacgatc tttagcataa gcaagggctt cgcgatatgc agcttcgcaa
     9481 acgccaacag actgggctgc aatacctaaa cgagcgccgt tcatcaatgc cattacatat
     9541 ttgatcaaac caagtttgcg ctgcccgcaa agttcagctt tggcattctt gaaaacaagc
     9601 tcgcaggttg gagaaccttt gatacccatt ttattttcaa tacgacgaac agtaacaccg
     9661 ccatcatttt tgtcatagat aaacattgaa agaccacgac cgtcggttgt cccttcttct
     9721 gaacgagcca aaacaagtga aatatggcca tctccattgg tgataaaacg ttttacaccg
     9781 ttcaataacc atgttccttg tttttcgtca taagtagctt tcaacatcac agcctgaagg
     9841 tcagaacctg catcaggctc tgttaagtcc atagccattg tttcgccttt gcaaacacgc
     9901 gaaagatagc gcattttttg ctcttcggtt gcaaactcgt tgatagtttc tgcgcagtcc
     9961 tgcaatcccc agatgttaac aaaaccgcag tcagcgcgtg acaccatatc tgcagccatg
    10021 atataaggaa caatagggaa attaagacct ccaaaacgac gaggtaatga catacccatc
    10081 aatcctgctt tgttcaatgc atcgaggttt acaactgtac ctggagcata ttgtacatgg
    10141 ccatcaacaa gctgaggccc atcatgatca acagattcag cattaggagc aatgatatcc
    10201 ccacagattt caccaacaat ttcaagaact ttctcaaaac tgtcgatagc atcttcataa
    10261 tcatgcggag catagtcgaa cttatctttc tgagtataat ttctttcttt caactctaca
    10321 atcctgcgca taagcggatg attgaggtgg aattttaatt ccggtgtatc ggtataaaaa
    10381 ttagccattt ttctatttat ttttagatcc aagaatcaag aatcaagaat caagaccttt
    10441 agtctatttt ctttaatctt ggctcatggc tcttggttct taactctatt tactattttg
    10501 tttgtaatat ttaatcatct tagggagcac atcttcaata ttgccggtaa ttacatagtc
    10561 tgcaatcttg ttgatagggg cattggggtc attgtttacc gagatgatca tgcttgactc
    10621 ttgcataccc gcaatatgtt gaatctgacc agagatacca caagcaatat ataactttgg
    10681 acgtacagta acacctgttt gtccaatctg acgctcatgt tcagcatatc cggcatcaac
    10741 agcagcacgt gatgcgccta cttcagcacc aataacagca gctaattcgt acaacaaagc
    10801 aaaattctct tttgagccaa caccataacc tcctgcaaca atgatagatg agtttttgat
    10861 gtttactttt gatttttcca tgtgacgctc aacaatgctg atggcaaagt ctgaagcaga
    10921 aacatatttg tcagcatcga gttttacaac ttcaccttta tggttggcat tgaaaatttc
    10981 ttttttcatt accccttcac gaacagtagc catctgagga cggcagtctg ggttcacgat
    11041 ggtagcaatg atgtttcctc caaaagctgg acgaatttga taaagaaggt ctttatattc
    11101 tttacctgtc ttggtttcgg tatgagggcc aatctcgagg ttagtacagt cagctgttaa
    11161 accgctcttc aatgctgaag atacacgtgg gcctaagtca cgacctgttg ttgttgctcc
    11221 catcaaacag atttgtggtt tttgttcttc aaacaattta acacaaattg aagtgtgagg
    11281 caatgtttgg taatgttcca gacgtttgtc atcgacaaca tacagcgtat ctacgccgta
    11341 aggaaaaacc tggcttccga tactgtctaa cttataacca atggcgagag cttcgagctt
    11401 gcatttcaaa tcctgagcaa gtttgcgacc tttggtaatc agctcaaggc tgacatcggc
    11461 cactttaccc tcttctattt cgcaatatac tacaatatta ttcatttctt ttaatttgaa
    11521 aatttgtgaa tttgaaaatt tgtgaatgtt tccattcatt gctaattaac aaattagcta
    11581 attatcctat agtgtgattt tcaataagtt ctttcatcaa ttgattgata tcgttatctc
    11641 catcagtgag cctctttgat tccttagcct gaaatacaat attttctatc ttttttactt
    11701 tggtaggtga accagaaaga ccaagttgtt ctctatcaca ttcgatatcg tttacactcc
    11761 actctttaat attgagataa ggcttgtcat tgatcatcgc caaataatct tcgcttttct
    11821 cagccctttc gctcatagta gaggaatgtt tgtatttcat caacaatttt gcgtttcgcg
    11881 gacgacattc ggctgctgaa ccatttacgg ttattactaa tggtaactta ccttcaacaa
    11941 cttcaatacc acgttccaaa cgacgtttga tttttattac attattcttt acagaaacaa
    12001 cttcttctgc ataagtaatt tgtggaatac caagtttttc ggcaacctga gggcctacct
    12061 gtgctgtatc tccatcaata gcctgacgcc cacagatgat aagatcgtaa tctttaactt
    12121 ttctgatagc acaagcaata gcatatgaag ttgccaaagt atctgcacct gcaaatgccc
    12181 tgtctgtcaa caaaatgcca tcatcggcac ctctgaacaa tccttcacgc aacacttctg
    12241 ctgcacgtac aggacccatg gtcaataatg taatgtgtgt acctggaaat tgatctttca
    12301 agcgtaaagc ttgttcaagt gcattcaaat cttcagggtt gaagatggca ggtaatgctg
    12361 ccctgtttac ggtcccgtct gctttcatgg catctttgcc cacgttacgt gtatccggaa
    12421 cctgtttagc taatacaatg atttttagtc ccattatctt aatttattag taaaattgtt
    12481 ttcaaaattc ggcggacaaa tataaacata actcaataaa taacaagggc atacaatttt
    12541 gaatggttat aaataaggta tatttattca caccctttca tttctcatta gaaaacaagg
    12601 agttataaag tttataaagc aataaaaaca aggtttttca tccgtaaata attttataaa
    12661 taaggtcgga tgaaccccga aaggctctcc gaaggaaatc tcgcatgatg aatatttatc
    12721 cttgtttgtg tttaaaaaat catgctcgtt tcatttgaaa aaaattttaa aataattcaa
    12781 aagtcaaaac tcgtttcaat tgttcaagat gttgattatt tttactcaaa attattaaga
    12841 accattatgt cgttagatac catcatagaa ttctgtaatg caaagatttg ccaacaggat
    12901 caccttgtaa tgagcaatgt aaacttcaaa gtcgataaag gcgaatttgt ttacctcata
    12961 ggcaaagttg gaagtggaaa atcttcgctg ataaaaacca tcaatgctga gcttcctttg
    13021 actgaaggaa ctggatttgt ggccggtttt gaacttgata aacttaaacc caggaaaatc
    13081 ccttatctca gaagaaaact tggtgttgtg tttcaggatt ttcagctgct tacagaccgt
    13141 actgtaatta aaaaccttga atttgtactt aaagccaccg gatggaaaga caaaaaagag
    13201 attgaaaaac gtattgccga tgtgttggaa aagacaggcc ttaccactaa aggctacaag
    13261 atgccgcacc aactttcagg aggcgaacaa caacgtgttt ctattgcccg tgctttgctc
    13321 aacgacccgg aaataatatt ggcagacgag cctaccggta atctcgaccc ggacacctcg
    13381 gccggtatca tgaacctgct atttgaaatc agccacaatg gccgtgccgt aattatggca
    13441 actcacaact acacgttttt aaagaacttc ccatctcgta tagttaaatg cgaaaatgga
    13501 acacttgtag aaaaagccca aagcgaagaa atagactttg aaacgttgat gaattaaggt
    13561 ttttgcaaaa caataatatc atttgatagt atcaatgtaa aagaagactt ttacatacaa
    13621 tcgagtcctc aatacttttt ttatcgtcca taaaaatatt acttttgcaa tactaaaaac
    13681 ttgaaattga gcaacaagcg tatggataga gatgagataa tcaaatacct gacaggacag
    13741 aaagaatatt tcaaccatcg gtacaacatt gcccaaatag gcttgtttgg aagtttcgcc
    13801 cgaaatacgc aaacaaaaga tagcgatatt gatattgttt atacaatggc tcaaaacaaa
    13861 aaacttagtt attatcaatt gtttgaaatt attgaaactt tggaaagcca ctttaaacgg
    13921 aatgttgagt tggtaaatga taaatatatg gaacccatta ttaaacatga cgcacaaaag
    13981 gatataatat atgtttagca ataaaaacct gatacatata cttacaattc tggaggctgc
    14041 cgagaaatgc tttatttata attgataatg ctgaagattt cttcaggcat aacgaccaac
    14101 ttaactacaa tgcttgtgtt acactgcttt tagcaattgg ggaagaaagt aaaagtattg
    14161 aagaaggttt aaagcaagaa ttcccgaata taaactggaa ggcaatagcc ggaatgcgaa
    14221 atatcatggc ccataattac agaggaattg acccggaaat ggtttttgaa attattgccg
    14281 aaaatctgcc aaccttaaag gaatgcatgt tgaatatgtt acagcgggtg cattttgaac
    14341 aagatttatt ggacaaagcc ctaaattcaa ttttctacac acatctttct tatttaaaaa
    14401 attgtaaatg atagaataga aactccaatg aattcttcat tacttaccaa acaacgcctt
    14461 caactccttc aaatcaacct taaagtcaac cagtttgaat tctgtgactt tgtaaaaata
    14521 atatgctaaa tatattgtag aagccagata agaaatactg gttgccacag cagccccttt
    14581 cattccatat attttcaaca ataaaggagc caggacaaca gtaaataaaa tacctaccaa
    14641 aga
//
LOCUS       DAOSPF010000071        14562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4167_length_14562_cov_6.030882, whole genome shotgun sequence.
ACCESSION   DAOSPF010000071 DAOSPF010000000
VERSION     DAOSPF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_14562_cov_6.030882"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PK252_12060"
     CDS             complement(<1..1961)
                     /locus_tag="PK252_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15486.1"
                     /translation="MSEKQGLPSTSVTAIVQDKEGFLWLGTYYGLHRYDGSHFKTYLS
                     SRQSDDSISDNFIKSLMVDKAGDLWIGTWGGGLNRYIREKDAFVSYRHNPADKHSISD
                     NLINTIYEDPQGNIWIATWGGGVNVIEAKYLKNNTLPQKISFVHYVYDSLNQNSLAND
                     NVNTIYQDSKTNIWIATKNGVSKWDAAKKLFNNIYHQRGNANSLSDNNCTSICEDKDG
                     NIWIGTWEAGLNKIEQPSNRVVRYRFDKSDKNGILSDKVYRLYKDFSGNLWIGTWGGG
                     LNQLIYNSEENNNQKAEKDKFYQFKNDPLDPNSISGNTIYAIFEDKTGVLWIGTANYG
                     LNKVDKRKMQFNHINIQPGQQNSMNDRAVRAFCIDKAGNLLVGTNFGGLNYFNKKTNT
                     WEHFVYQPENPYSISNNSIKAIFTDKKGRIWIGTESGLNRFDLANKRFYRFYYDPAKA
                     SDTHIQVICEDRFGYLWLGNYDGGLVRYDPVKNAFRQYLHNPLDSASIGSDIISSIIE
                     DRQGVLWIATVGGGLNKYDREHDCFVRYSYDAQNSLSISSNEVNVIFEDKKGELWIGT
                     SRGLNKMLNSNKKPSFKLFNIENNGLVDGYICSILEDNNENLWISTSKGLSRFNKVSY
                     KVKTFNVSNGLQGSEFLPNSAYKDKSTGM"
     gene            complement(2731..3309)
                     /locus_tag="PK252_12065"
     CDS             complement(2731..3309)
                     /locus_tag="PK252_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15487.1"
                     /translation="MLLLLVLLVILFSTSCIYAQNNKDSVIEPKGPTYMKVQYAGNIG
                     LLSVGMGQTYMDDKLYVDLNYGYLPKRLNGVRVHSFAIKASYQFVQGQLSSLELGSYA
                     GMCVLYGITSNTYIRYPDLYPDDYYLPNALHLMPLVGCNARCLSVDKRVSRVALYSEL
                     GTVDYKIWYALKNKKIKPADIFNFSFGVRYKM"
     gene            complement(3317..4060)
                     /locus_tag="PK252_12070"
     CDS             complement(3317..4060)
                     /locus_tag="PK252_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT15488.1"
                     /translation="MIVFCSCSDMIEYSPYDANVKTSVFNTVRAENISLVAFDEDTLK
                     FAVFSDSHNDYDKLSEIINKINLQKGLQFVVCCGDVTKAGLSQEYIWYLDAVEHCKYP
                     IFTVIGNHDYRSNGYVIFNKVFGQPNMSFIVGRYKFVMFDDIIWENNNKSPKYEWLKE
                     EMADSAYKHIVLAHIPPWSDQMEGLNKLVFDQIVTPQNTILCIYGHDHSFLDKKVNGI
                     HSIVSGCVKDKSCFVVSIVHDKAIIEQLN"
     gene            complement(4834..9279)
                     /locus_tag="PK252_12075"
     CDS             complement(4834..9279)
                     /locus_tag="PK252_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT15489.1"
                     /translation="MGYKKKSIIESAMRNRNIMMILSAVFMIIGGIALVKMPRNEFPD
                     FTIRQGVIVGVYPGATSAEVEEQLTKVVENYIFGYKEVKKAKTYSHSKEGIMYIFVEL
                     NDDVKNADQFWSKLKHGLAELKMTLPTGVLALVANADFGDTSALLITLSSDTKSYKDL
                     EEQLKKLETEVRKIPATSKVKHFGLQKEKIFVNVKPEKLNEYNIKALSLLGAYQTNGM
                     INYAGVLKDGKTNLSVHLPANFESEKDLADQIVYADPNGNVVRLKDIATIERRYEDPD
                     SYIKQNGMKTILLSLEMQTGNNIVQYGKEVDKAIAKFQKSCPEDIKVAKISELPKYVQ
                     DSVNNFMREFFIAIVAVILVTMLLLPLRVASVAGITVPISVFITLGFLYFFGVELHTV
                     SLASLILVLGMIVDNSIVVIDNHVEKIDHGISPWHAAIASAKELFSPIVTATLAIIVV
                     YIPMALMVPGTAGEFLMTIPIVVAIALTLSIVIAVLLVPYLNFVFIKKGLKTQENKKK
                     GKTFLDWLQTKFDNSIEKVFRHPRMVFGAAVILIIFSAILFKNTDQQLFPELERNQFA
                     VEVYLPTGASLESTAAVMDSMENLLLKDKRVTNVTSFIGTSSPRFQVAYAPNMPASNY
                     GQLLVNTVSDKATRAIVNDYSEKYANRFENAHVKFKILVLQLNKYPIEIRISSDSIKD
                     IRAVEAKVDSILKNTKHIAWMRTDWDQKQQSVKVNLDRDKANRMGYSKGLVATSLMFG
                     LDGIPLTTIWEDDYPVEVRLSQETKESKSITTIENQYITSPASFTALPLRSFASFSPE
                     WTEGTIVHRNGTPTLTISIDNDLTGIAAKMFDEIKPQIDKLELPEGTSINYGGDYEGQ
                     QDVFTPMTIALVLSIVLIFFILLFQFKKEKLSLLIMSSMLLTLPGAVLGLKLMGYPFS
                     LTAFIGISSLCGMVVRNGIILIDYARDLHENKGISVHEAAIAAGKRRMRPIFLTSAAA
                     SVGVIPMIISRSPLWGPLGSVICFGLLVSMILTLYILPILYSWTYSDKVKKPGFWSVP
                     SGKFVKGASVGLLLFIFLPNKAYAQVLTLDSCKVLALQNNYQIKEAVSDVVQSEQVKK
                     NAFTNYFPKVSASATAIKLNDYLIKAKIPQMNLPVYNGNPATLATATQFAYFPETPVN
                     VLNYLNIANVIVVQPLFAGGRIINGNKLAQAALDINREKQAMTTTEVLVKTEELYWSV
                     INLGEKLKTLDSYIQLLDTLYKDVNVAYKAGLVQRTDLLKVQLKQNELMVDRIKLTNG
                     IALGKMALCQHIGCKYDSAMVLDSSTLKIESPQIYVDNNELVVTNRNEYKMLEKAIKA
                     EELQKRMVAGEYLPQLSVGAAGTYADAMNSKKTNGLVFATLSIPISDWWGGAHKIKES
                     KAKIESARYKMAETSELMQLQITQAKNALNEDYFQVSIAEKSVEQAKENLKVTSDNYK
                     AGVSGMSDLLEAQSAYLNAQNSLTEAKCNYQIKKARYLQAINNYK"
     gene            complement(9281..10411)
                     /locus_tag="PK252_12080"
     CDS             complement(9281..10411)
                     /locus_tag="PK252_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT15490.1"
                     /translation="MKYLSSCKRNQYENECFSPKKGQAIIPYKLNIIMMKKLALLTLV
                     MLVCASCSQLTENPANEKQAISVVTQKVERVKCSTNLRYSGTVEASQTIPLTFQTTGI
                     VEQVYVQEGDAVSKGQLLAVLDKNDLQNIYDASYSKYQQAKDAYDRLKTVYDQGSLPE
                     IKWVEMQTNFEQAKSSKDLAKNNLDKCYMRAPESGIIGRRNIEPGQSAFGLSSAPFEL
                     VKINQVMVKIAVPENEISKVRKGLKASFSVSALDERRFEGTITNVSPVADAISRTYTA
                     KILVNNPKHELLPGMVCDVELDVKLEKDVLTVPYSAVTTGNDGKPFVFVVSGDKKTVK
                     KQIVETGNYQNSNIEILNGLTEGQTIVVVEKEKLSDNSLIRL"
     gene            complement(10526..11125)
                     /locus_tag="PK252_12085"
     CDS             complement(10526..11125)
                     /locus_tag="PK252_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT15491.1"
                     /translation="MLVVMNDTKKYIIDKAFELFLSKSYEAVSISDISQAIGFTKGAL
                     YHHFTNKEELFKAVIDKYLTFVGVEADLDKVSLERYNELIIANVEKMLNKLFSNKAQF
                     VPINYLTLIADGFRHYKGFAEEKEVFFNTEINKTKAVLDSLVKRGEIRSDIDTLAIAR
                     IYFSSTIGLAGNMLQNYSLQESLKSLKDQLSELAKLLKV"
     gene            complement(11250..12398)
                     /gene="dinB"
                     /locus_tag="PK252_12090"
     CDS             complement(11250..12398)
                     /gene="dinB"
                     /locus_tag="PK252_12090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT15492.1"
                     /translation="MIVTNRNIVHLDLDTFFVSVERLRNSKLNGIPVIVGGQSDRGVV
                     AACSYEARQYGVHSAMSMKMARMLCSDAVFIRGDMDLYSRYSHTVTDIIAEAAPVYEK
                     ASIDEHYLDVTGMDKFFGAYKWAHELRQRIILETGLPLSLGFSVNKTVSKIATGKAKP
                     NGELQVPAPEVLSFLQPLPINKMPGIGEKTGQLLRSMGIVTLGMLQQMPGEMLESVLG
                     KPGLTIWKKANGIDNTPVEPYSEQKSMSREQTFEQDTIDVDLLKRILTGMVEQLAYDL
                     RREQWLTSCITVKVRYSNFDTHTLQKRVPYTAYDDELIAEAKSLFDRLYQRRMLIRLI
                     GVKFSQLVRGTKPLGLFDNSQQQSRLYSAIDSMRKRYGPGVLHRAISD"
     gene            12660..14528
                     /locus_tag="PK252_12095"
     CDS             12660..14528
                     /locus_tag="PK252_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT15493.1"
                     /translation="MNEVPFYKSLKFYLCLFSSLCGTVFLVQLGSFILNNNHQGFSLI
                     LIIAGLIISGFNIYFVTSRLNRELHILQQSIHDVILHRFSRKFKLKTVPAKDEIGQIS
                     HEVGLILDKAHQRTTEVMLRRQEVEMANRNMEQLAAMGREIISKLDITRIIQTTFNSV
                     NNLMEASLLSVGIYNEIKQGLDLFGIRAGNNEVITGFDDMSDTLHWNVHVFNTQEEIL
                     CNEFDCRRDKHFSSILFNDPIEWRESFIYLPLTYNQVRIGVFTVQSNKKNAYNTYHLG
                     LMRNLANYMTIAFQNAYAFRKIEQQKELLEETSRQLKDINDNLELQVHLRTIEITRQK
                     EEIEKKNIALERLSLVATKTENAIMIMDAQGNIQWLNECFLRIYNYTFEEFIEKRGNN
                     LLNTSFHPDIKKIFDKCRSTKKPVYYEALNIKGDGSSIWTQTTLTPVLNEANEITHLL
                     TIDSDITRQKEYEIAITKQTEDITNSIIYAKRIQSAILPCREKFESAFNDYFLLYKPK
                     AIVSGDFYWIDQRNGKTLFAIADCTGHGVPGAFMCMLGVSLLNQIVQTMENISADEVL
                     NTLRNNIKASLQNTETSDGMDIGFCIYDPQTNTLEYSGAFISLIMIRNNEISLLSH"
BASE COUNT         4514 a         3122 c         2478 g         4448 t
ORIGIN      
        1 atcatgcctg tactcttatc tttataggcc gagttaggca aaaattcgct tccctgcaaa
       61 ccattgctca cgttaaatgt ttttacttta taagaaactt tattaaacct cgacaatcct
      121 tttgaagtgc taatccaaag gttttcatta ttatcttcga ggatactgca aatatatcca
      181 tcaaccaaac cgttattttc aatattaaaa agcttgaacg atggtttttt attgctattg
      241 agcattttat tcaggccccg cgatgtgcct atccacaatt ctcctttttt atcttcaaaa
      301 ataacattga cctcatttga gcttatcgaa aggctatttt gagcatcata gctataccgg
      361 acaaaacaat catgctctct gtcgtattta tttaacccgc cacccacagt agcaatccat
      421 aaaacgccct gacggtcttc tataattgaa gaaattatat cgctgccaat acttgcagag
      481 tctaaaggat tatgtaaata ttgtcggaag gcgtttttaa ccggatcata cctgacaagc
      541 ccgccatcat agttgccaag ccaaaggtaa ccaaacctgt cctcgcaaat aacctgaatg
      601 tgagtatctg aagctttggc agggtcatag taaaacctgt aaaaacgctt attggccaaa
      661 tcaaacctgt tcagccccga ttcggtgcct atccatattc tgcctttttt gtcggtaaaa
      721 attgctttta ttgaattgtt gctaatacta taaggatttt caggttgata tacaaaatgt
      781 tcccaggtat ttgttttctt attaaaataa ttaagccctc caaaatttgt acccaccaaa
      841 aggttgccag ctttatcaat acaaaatgca cgtacagccc tgtcattcat actattttgt
      901 tggccaggtt gaatgttgat gtggttaaac tgcatttttc ttttatcaac tttattgagc
      961 ccataattgg cagtacctat ccacaatacc ccggttttat cttcaaatat ggcataaata
     1021 gtattgccac taatgctgtt gggatcaagc gggtcattct taaattgata aaatttgtct
     1081 ttttctgctt tttgattatt attttcttca ctgttataaa tgagttgatt aaggcctcca
     1141 ccccatgtac ctatccagag gttgccagaa aaatctttgt acaacctata taccttatca
     1201 cttaaaatgc cgtttttatc gcttttatca aacctgtacc tgacaaccct gttagaaggt
     1261 tgttctattt tgttcagccc ggcttcccat gtgcctatcc aaatatttcc atctttgtct
     1321 tcacaaattg aggtacagtt attatcactc aggctattag cattaccgcg ttggtgatat
     1381 atattgttaa aaagcttttt agctgcatcc catttgctta ccccgttttt agtagctatc
     1441 cagatattcg ttttcgaatc ctggtaaata gtatttacat tatcgttggc taaagaattt
     1501 tggtttaatg aatcataaac ataatgtaca aagctaattt tttgaggcaa agtattgttt
     1561 tttaaatatt ttgcttctat cacgttgaca cctccccccc aggtagcaat ccaaatattt
     1621 ccctggggat cttcataaat ggtatttatt aaattatcgc taatactatg tttatctgcg
     1681 ggattgtggc gataagaaac aaaagcatct ttttctctga tgtacctgtt aaggcctccg
     1741 ccccatgtgc ctatccaaag atccccggcc ttgtccacca ttaacgattt gataaaatta
     1801 tcgctaatac tatcgtcact ttgtctgctg cttaaataag ttttaaagtg gcttccgtcg
     1861 tatcggtgta agccataata tgtacctaac cacaaaaaac cttctttatc ctgcactatt
     1921 gctgtaactg aagttgaagg caacccttgt ttttcggaca aatgttcaaa gtttaattta
     1981 ctctgtgagt gcagaccaat gcataataaa aggaatagac ctgtaaaata tttggggggg
     2041 catttaatgg tcagcataag tgttttctta ttgtccttat aaatttaata tacttttttt
     2101 taatctacat acattctttc catttttaat aacatattaa actttatatt ttgagcaaaa
     2161 caaagaaact atcctgcctt ttaaaggctt ttaaattacg tcatttaagc agttaattct
     2221 actttgaata tattgaaacc atattgtccg gtaaataaaa tagatactta taaatactag
     2281 taatattgtt gattagaaac tggtcgcccc gccagggctt aattttaacg atgcttaatt
     2341 ttactacaaa caggacgtcg ctaagcgact aaataatagc agcgtagctg cgaactgttt
     2401 gtaggacaac caaatacacc caatgcttct aagccgcata gcggcgaact gtttgtaccg
     2461 gacaacaatg acattgaaat acaattacat atagttaaat agttaaaata taaaggtaaa
     2521 aaagcaacgt agttgcaaca tctttgtagt tcaataaaac tgccatagtc atcaggtacg
     2581 atgcaccgat atagcatatt atatgtttcg ccactaagag gcttaacatt tacaggcaac
     2641 aattttctaa aaagatttcg cagcaatgcg gctaacagca agggttaact aatctgtcaa
     2701 ggtagaatta actatctgta cgataaaaaa tcacatttta taccttaccc caaaactaaa
     2761 attaaatatg tcagcaggct taattttctt gtttttcagg gcataccaga ttttataatc
     2821 aacagttcct aactctgaat acaacgcaac acgactaact ctcttatcaa cagacaagca
     2881 ccgggcatta caccctacca aaggcatgag gtgcaaggca ttgggcaaat agtagtcatc
     2941 aggatataaa tcggggtaac gaatgtaagt gttcgaagta atgccgtaca atacacacat
     3001 gccggcataa gagcccaatt caaggcttga caactgtcct tgcacaaact ggtacgatgc
     3061 ttttatggca aaagaatgta ccctcacgcc attgagccgc ttgggcaaat atccataatt
     3121 gaggtcaaca tacaacttgt catccatgta tgtttggccc atccctacag aaagcagtcc
     3181 aatattgccg gcatattgta ctttcatgta agttggcccc ttaggctcta ttactgaatc
     3241 tttgttgttt tgtgcataaa tgcagcttgt agaaaacagt atgaccaata agacaagtaa
     3301 taataacatt ttaaggttaa tttaactgtt caataatagc cttatcatgg acaatactaa
     3361 caacaaagca gcttttatcc ttaacacagc cagaaactat actatgaata ccgttcacct
     3421 ttttatcaag aaaactatga tcatgcccat aaatgcataa aatggtattt tgtggagtta
     3481 caatctgatc aaatacaagc ttatttaagc cttccatctg atctgaccag ggggggatat
     3541 gggccaatac aatatgtttg taagcgctgt cggccatttc ctctttcaac cactcgtact
     3601 taggcgattt gttgttgttt tcccaaataa tatcatcaaa catcacaaac ttgtaccgac
     3661 caacaataaa ggacatattg ggttggccaa ataccttatt gaagataaca tagccatttg
     3721 agcgatagtc gtgattgcct attacagtaa atattggata tttgcaatgc tctacagcat
     3781 caaggtacca aatatactcc tgggacaaac cggccttggt tacgtcgcca caacaaacaa
     3841 caaattgcaa tcccttttga aggttaattt tatttatgat ttcagacagt ttatcgtaat
     3901 cgttatgtga atcagaaaac acggcaaact taagagtatc ttcatcgaat gcaactaatg
     3961 atatattttc agcccttact gtattaaaaa cacttgtttt tacattagca tcgtaagggc
     4021 tatactctat catgtcagag catgaacaaa aaactatcat gtttatgcac gctataaaga
     4081 ttaggcttcg taatattttt aaattaaatg gcatgcttga aatttttaaa aataaagctt
     4141 tcattttttg ttttgcatta aagtagttta ttttactact tagaccttcc aaatttttga
     4201 aaactcaaag gccacctgac taattcaaca cgtatatata tgaaacatca aacaaatttg
     4261 tataataata aaaaaacaaa aattagaata tcggcaaaat ggacaaaaaa ggggttaaaa
     4321 cacttacaat cagcagtcaa atgtgactaa aaaaaacata caactaaaac aaaactgccc
     4381 tctttattaa tcatcatcat tgttttttta cttttgcaaa aaaggccccg ggaaaccaag
     4441 aacaattaaa tagaatgaaa aaccaaacaa aaacaacccg gggacctttt atattctata
     4501 tattatatcc ttctaaacaa gacaaaccca ttatttatct ctttattagc ccaagccttt
     4561 aaccagtcat caataaagtt tgaacaaaaa tgataatcat taaaataaaa agaaataaaa
     4621 catttcctta agtggacaaa ataaggtata aagtgactat tgtcaccgtt gaattttgtt
     4681 atattatttt tactaaacca gttcataaac tttattaaaa tatttacctt tttaaatcaa
     4741 aaacagcatc ttaaagaaag gccccgggaa accatgaaca attaaataga atgaaaacca
     4801 aaaccaaaaa aacaaacccg gggccatgtt tttctattta taattattta ttgcctgcaa
     4861 atagcgggct ttctttatct ggtaattaca ttttgcttcg gtaaggctgt tctgcgcatt
     4921 aagataagca gactgcgcct caagcaaatc tgacatgccc gacacaccag ctttatagtt
     4981 atctgaagta accttgaggt tttctttagc ctgttctacg gacttttcgg caatagaaac
     5041 ctgaaaatag tcttcgttca gggcattctt agcctgcgtt atttgtagtt gcatcagctc
     5101 agaagtttcg gccattttat atctggcgct ttctattttt gctttacttt ctttaatctt
     5161 gtgagctcct ccccaccagt cgctaatagg aatactaagg gtagcaaata caagcccgtt
     5221 agtcttttta ctattcattg catcggcata agtaccggca gcaccaacag acaactgcgg
     5281 aagatattcg ccagcaacca tgcgtttttg aagctcttct gccttaatgg ctttttcaag
     5341 catcttatat tcatttctgt ttgttactac caactcgtta ttatccacat atatctgagg
     5401 gctttcaatt ttcaaagttg agctgtcgag aaccattgcg ctatcgtact tacatccaat
     5461 atgctgacac aaagccattt tgcccagagc aataccgttg gtaagtttta tacggtcaac
     5521 cattagttcg ttttgtttta gctgcacttt taagaggtct gttcgttgaa ccagcccggc
     5581 cttataagca acattgacat ctttgtatag cgtgtcaagc aattgtatat aactatccag
     5641 agtctttagt ttttcaccca aattgattac agaccagtac aactcttcag tttttacaag
     5701 cacttcggta gttgtcatag cttgtttctc gcggtttata tccaaagcag cctgagccaa
     5761 cttgttacca ttgataatgc gccctcctgc aaacaatggc tgtacaacaa taacattggc
     5821 tatattcagg taattcaaaa cgttgacagg tgtttcaggg aaataagcaa attgtgttgc
     5881 cgtagctaaa gttgcaggat tgccattata aaccggaagg ttcatttgcg gaattttggc
     5941 ttttatcagg taatcgttca gttttatagc agtagcgctg gcgctcactt tgggaaaata
     6001 attggtaaaa gcgttctttt taacctgttc agattgcact acatcgctta ccgcttcttt
     6061 tatttgataa ttgttttgca gggcaagcac cttacacgaa tctaatgtca acacttgtgc
     6121 ataagcttta ttgggcagaa aaataaacag caacaagcca acggaagccc ctttgacaaa
     6181 ttttcccgaa gggacagacc aaaaaccagg tttcttgact ttatcactat atgtccacga
     6241 atataaaatt ggcaggatgt acaaagtcaa tatcatagac accagaagcc caaagcaaat
     6301 aacagagccc aatggccccc atagaggcga acggcttata atcataggaa taacacctac
     6361 cgaggccgca gcagaagtaa ggaatattgg gcgcatacgt cgcttaccgg cagcaatagc
     6421 agcttcatgg accgatatcc ccttattttc gtgtaaatcg cgggcataat caataaggat
     6481 gatgccatta cggaccacca taccgcaaag acttgaaata ccgataaaag ctgttaagct
     6541 aaacggataa cccatcaatt tcagaccaag tactgccccc ggaagtgtca acagcataga
     6601 tgacataata agcagcgaaa gcttttcttt cttgaactgg aataacaaga taaagaatat
     6661 gagcacaatg cttaaaacaa gcgcaatggt cattggcgta aacacatcct gttgcccttc
     6721 ataatcgccg ccataattga tagaagtacc ttcgggaagc tcaagtttat ctatctgagg
     6781 tttaatttca tcaaacatct tggctgcaat acctgtcaga tcattatcta tagatatagt
     6841 caacgttggg gtgccattgc ggtgcacaat ggtcccttct gtccactcag gcgaaaaact
     6901 ggcaaaagag cgtaatggta atgctgtaaa acttgcagga gaagtaatat actgattttc
     6961 tatagttgtt atgctcttac tttctttggt ctcttgcgac aaacgaacct caaccgggta
     7021 atcgtcttcc catatagtag ttaaaggtat tccatcgagc ccaaacataa gcgatgtagc
     7081 cacaaggcct ttggaataac ccatcctgtt tgccttgtcg cgatcaaggt tcacctttac
     7141 cgattgttgc ttttggtccc agtctgttcg catccaggct atatgcttag tgttttttag
     7201 gatactatcc accttagctt ctacagccct gatatctttt atcgagtcgg agcttatccg
     7261 tatttctata gggtatttat tgagctgcaa taccaatatt ttaaatttta catgggcatt
     7321 ttcaaacctg ttggcatact tctcgctata atcgtttact atagcgcgtg tagccttatc
     7381 cgaaacagta ttgacgagca actggccata atttgatgct ggcatattgg gggcataagc
     7441 cacctgaaaa cgaggcgaac tggtaccaat aaaactggtt acattcgtaa cacgtttatc
     7501 tttaagcaac aggttttcca tgctatccat cacagcagca gtactttcga gtgatgcccc
     7561 tgtgggcagg taaacctcaa cagcaaactg gttgcgctct aattcaggaa atagttgctg
     7621 atcggtattt ttgaacaata tagccgaaaa tattattaat atcactgctg ccccaaagac
     7681 cattctgggg tgtcgaaaaa ccttttctat cgaattgtca aactttgtct gcaaccaatc
     7741 gaggaaagtt ttgcctttct ttttattttc ttgtgttttc aaaccttttt tgataaaaac
     7801 aaagtttagg taagggacca gcaatacggc aataacaata gacaatgtca gggcaatggc
     7861 aacaacaata ggtattgtca tcagaaactc cccggcagta ccaggtacca taagcgccat
     7921 aggaatgtac accactataa tggcaagtgt tgctgttact ataggagaaa atagttcttt
     7981 tgcacttgct attgcagcat gccaaggcga aatgccatga tcaatctttt cgacatggtt
     8041 atcaatgacc acaatagaat tatcgacaat catccccaac accaggatca acgaagcaag
     8101 tgaaaccgta tgaagttcta caccaaagaa atataagaaa cccagtgtga taaaaaccga
     8161 aataggcaca gttatgcctg ctaccgaagc tacccttagc ggcaacaaca acatggtaac
     8221 cagaataaca gctacaattg caataaaaaa ctctcgcata aagttgttta cggaatcctg
     8281 aacatatttg ggcaactccg aaatcttagc aactttgata tcttcaggac aacttttctg
     8341 aaatttagct atagccttgt ccacctcttt accatattga acaatgttgt ttcctgtttg
     8401 catttctaat gaaagcaaaa tagttttcat cccattctgt ttgatatagc tgtcggggtc
     8461 ttcgtaacgc ctttcgatag tggcaatatc ttttagcctt actacgttgc cattggggtc
     8521 ggcatataca atctggtctg ccagatcttt ttctgactca aagttagctg gcaaatgtac
     8581 agagaggttt gttttaccat ctttcagcac acctgcatag tttatcatac cattagtctg
     8641 gtaagcgcct aacaatgata gtgcctttat attgtactca ttgagttttt cgggtttgac
     8701 atttacaaat atcttttctt tttgcaaccc aaaatgtttt actttagaag tagccggtat
     8761 cttgcggact tctgtttcta acttcttgag ctgttcttcc aaatccttat aactctttgt
     8821 atctgaagat aaggtaataa gcaaagctga agtgtcgcca aagtctgcat tggcaaccag
     8881 cgccagcacc ccggtgggca gggtcatttt tagctcggcc aagccatgtt ttagttttga
     8941 ccaaaactgg tctgcattct ttacgtcatc gttcaattct acaaaaatgt acatgatccc
     9001 ctctttagaa tgcgagtatg tcttggcctt tttaacttct ttatatccaa aaatgtagtt
     9061 ttcaactacc tttgtcagtt gttcttctac ctcagccgaa gtagctcccg gatatacccc
     9121 aacaataacc ccctgacgta ttgtaaaatc cggaaactcg ttacggggca tctttacaag
     9181 tgcaatacca ccaataatca taaataccgc agaaagtatc atcatgatat ttctgttccg
     9241 cattgcagac tcaataatgc tctttttttt gtagcccata ttacaaacgt attaagctgt
     9301 tatctgacaa tttttctttt tcaactacta caatggtttg gccttctgtc agcccattta
     9361 atatttcaat attggaattt tgatagttgc cggtttctac tatttgcttc ttgacagttt
     9421 ttttgtcacc cgaaacaaca aatacaaagg gctttccatc attaccagtt gttacagccg
     9481 agtaaggcac agttaaaacg tctttttcaa gctttacatc cagttcaaca tcgcagacca
     9541 tacctggcaa aagctcgtgt tttggattgt ttactaatat tttagcggta taggtacgag
     9601 aaatggcatc tgccacaggg ctaacattgg ttattgtacc ttcaaacctt ctctcatcca
     9661 atgccgaaac actaaacgaa gctttaagcc cttttctgac cttgcttatt tcattttcgg
     9721 gcacagcaat cttcaccatg acctggttta tctttaccag ctcaaaagga gccgaagaca
     9781 aaccaaatgc agactggccc ggttcaatat tgcgcctgcc aataatacca ctttcgggcg
     9841 cgcgcatgta acacttgtct aagttgtttt ttgcaagatc ctttgaagac ttggcctgtt
     9901 caaaattggt ctgcatttca acccatttaa tttcgggcaa acttccctga tcatacactg
     9961 ttttgagcct atcataggca tcttttgctt gttgatattt tgaataagaa gcatcgtata
    10021 tattttgcaa atcgttttta tctaaaacag caagcaactg ccctttagac acagcatcac
    10081 cctcctgaac atacacttgc tctacaatgc cggtagtttg aaatgtaagg ggaatagttt
    10141 gcgatgcctc cacagtaccg ctataacgca ggttggtact gcattttaca cgctctacct
    10201 tttgtgttac caccgaaata gcttgttttt cgttggctgg gttttcagtt aattgtgagc
    10261 aactggcaca aaccagcatt actaaggtta ataaagctaa ttttttcatc ataataatat
    10321 ttaatttata aggaatgata gcttgcccct tcttgggcga aaaacactca ttttcatatt
    10381 ggttgcgttt acaagaactt aggtatttca tggtgttcta ttttttatta atactaaacc
    10441 tttaattttt ttattttaaa aacatactga acgaatagta tgtttttgct aaataaaaaa
    10501 ctggtttact ttatctaatc aaaaactata cctttaataa tttggccaat tccgataact
    10561 gatctttcag cgattttaaa gactcttgca acgagtaatt ttgcaacata ttaccagcaa
    10621 gcccgatagt acttgaaaaa taaatccttg caatagccag ggtatcaata tcactgcgta
    10681 tttcgcccct ttttaccaaa ctatcaagca ctgctttggt tttgttaatt tcggtattga
    10741 aaaacacttc tttttcttcg gcaaaacctt tgtaatgcct aaaaccatca gctatgagtg
    10801 tcaggtaatt gatggggaca aattgcgctt tattactgaa aagtttattc aacatctttt
    10861 caacattggc aataataagc tcgttatacc tttccagcga aaccttatca agatctgctt
    10921 caaccccaac aaatgtaagg tatttatcaa taacagcctt gaaaagctct tctttattgg
    10981 taaaatgatg atataacgcc cctttggtaa aaccaatagc ctggcttata tcgcttatcg
    11041 aaacagcttc gtaacttttg ctcaaaaaca gctcaaaagc tttatctata atatattttt
    11101 tagtatcatt catgacaacc aacatactga acaattggta tgcaaatata ggagaagaaa
    11161 tttacagcaa cacacttttt ttgtaaaaat ttcttaaaaa gcagaaaatt caaaattagt
    11221 acctgctcaa caatatctac ccctccctat caatcactga tagcccggtg caacaccccg
    11281 ggcccataac gtttgcgcat actgtcaata gcactgtaca gcctgctttg ctgctgactg
    11341 ttatcaaaaa ggcccaaagg ctttgtaccg cgtactaact ggctaaactt cacgccaatg
    11401 aggcgaatca gcatacgacg ctgatataac ctgtcgaaaa ggcttttagc ctcggctata
    11461 agctcatcgt cataagctgt ataaggcacc cgtttttgca aagtatgcgt gtcgaagttt
    11521 gaatacctca cttttacagt gatacaagaa gtaagccact gctctcggcg caaatcataa
    11581 gccagctgtt ctaccatacc tgtcaatatc cttttcagca gatcaacatc aatggtatct
    11641 tgctcgaaag tctgttcgcg gctcatcgac ttctgttcgc tatatggctc aaccggtgtg
    11701 ttatcaatac cattggcctt tttccagatg gtcaggcctg gtttgcccag cacactttcg
    11761 agcatctcgc cgggcatttg ttgcaacatc cccagggtaa caatgcccat cgaccgcaac
    11821 aattgtcccg ttttttcgcc aataccgggc attttattga taggtaaagg ctgaagaaaa
    11881 gaaagcacct caggtgcagg aacctgcaac tcgccattgg gcttggcttt gccggtagct
    11941 atttttgaaa cggtcttgtt taccgaaaaa cctaatgata atggcaggcc tgtttccagg
    12001 ataatgcgct gccgcagctc atgcgcccat ttgtacgccc caaaaaactt atccattccg
    12061 gtaacatcaa ggtaatgctc gtctatcgaa gctttttcat acacaggtgc agcttcggct
    12121 ataatatctg taacggtgtg cgagtaacga ctgtacaaat ccatatcgcc ccggataaag
    12181 accgcatcac tacacaacat gcgggccatc ttcatagaca tggccgaatg tacaccatat
    12241 tgccgggcct cgtaactaca agctgccact acgcccctgt cactctgccc gccaacaatg
    12301 acaggtatgc cattgagctt gctgttgcgc aagcgctcta ccgatacaaa aaacgtatct
    12361 aaatctaaat gtactatatt tctgtttgtt acaatcattt tactttgcaa aatgattaat
    12421 ttgtaatcat tctgcaagat gttaaattaa tctaaacatt acgaaatagc aagcagattt
    12481 tgataaaaag aaattctaat attttttcat aaaatcatat aataaaaata aaacattaac
    12541 acaaaaagtt gtttatatta acaaatttaa tcaatacagt atataatatt gacatttatc
    12601 atcttttcaa tagtgatact taaaattatt aatatatttg caaaccaact taagtcgcaa
    12661 tgaacgaagt gccattttat aagtcattaa agttttattt atgcttattt tcttcgctct
    12721 gtggcactgt ctttctggtt caactgggct cttttatcct gaacaacaac catcaaggct
    12781 ttagcctaat actcattatt gctggcctta tcatttccgg gtttaacata tactttgtaa
    12841 cctcaaggct aaacagggag ctgcatattc tgcaacagtc catacacgat gtaatactcc
    12901 accgcttttc aagaaaattt aaacttaaaa ctgttcctgc aaaagacgaa ataggacaga
    12961 tatcgcacga agttggcctc attcttgaca aagcccacca acgtactacc gaagtgatgc
    13021 tcaggcgaca ggaagtagaa atggccaacc gcaacatgga acaactggcc gctatgggcc
    13081 gcgaaatcat ttcaaaactg gatataaccc gcatcataca aaccaccttc aacagcgtca
    13141 acaacctgat ggaagcctct cttttatcag tgggcattta taatgaaata aagcaagggc
    13201 tcgacttgtt tggtatcagg gccggtaaca acgaagtaat aaccggattt gatgacatgt
    13261 ccgacacact acattggaac gtccatgttt ttaataccca ggaagagatt ctctgcaacg
    13321 aattcgactg caggcgcgac aaacactttt cgtccatcct gttcaacgac cccatagaat
    13381 ggcgcgaatc gtttatttac ctgcctttga cttataacca ggtcagaatt ggcgttttta
    13441 cagttcaatc gaacaaaaaa aatgcataca acacttacca tctgggctta atgcgcaacc
    13501 tggccaacta catgaccatt gcttttcaga atgcttacgc gttcagaaaa atcgaacaac
    13561 agaaagagct gcttgaagaa acctcccggc aactgaagga tataaacgac aacctcgagc
    13621 tgcaggtcca tctgcgcacc attgaaataa cccgacagaa agaagaaata gaaaagaaaa
    13681 acattgccct ggaacgcctc tcactggtag ccaccaaaac cgagaatgcc attatgatta
    13741 tggacgcaca gggcaacata cagtggctca acgaatgctt tttgcgcatc tacaactaca
    13801 cctttgagga atttattgaa aaaagaggca acaacctcct gaataccagt tttcaccccg
    13861 acattaaaaa aatttttgac aaatgccgtt ctactaaaaa gccggtgtat tatgaagcgc
    13921 taaacatcaa aggcgacggt tcttctatat ggacacaaac cacccttacc ccggtattga
    13981 acgaagcaaa tgaaattacc cacctgttga ccatagactc agacattaca cgacaaaaag
    14041 aatacgaaat tgccattact aaacaaaccg aagatattac caattctatt atctacgcaa
    14101 aacgcataca atcagccatt ttgccatgcc gcgaaaagtt tgaatcggcc ttcaacgact
    14161 atttcctgtt atacaagccc aaagcaattg ttagtggcga cttttactgg atcgaccaaa
    14221 gaaacggcaa gaccctcttt gctattgccg actgcacggg gcatggcgtt ccgggagctt
    14281 tcatgtgcat gttgggcgtt tcgctgctaa accagatagt acaaacgatg gaaaacattt
    14341 ctgctgatga agttttgaac acattaagaa ataatattaa agcctcattg caaaataccg
    14401 aaacatcaga tggcatggac atagggtttt gcatttacga cccacaaaca aacacacttg
    14461 aatactcagg cgcttttatt tcccttatca tgataagaaa taacgaaatc tcattactgt
    14521 cccattaaaa tctaaaagag ttctttgaaa agtttgaaat tt
//
LOCUS       DAOSPF010000072        14475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4198_length_14475_cov_6.255132, whole genome shotgun sequence.
ACCESSION   DAOSPF010000072 DAOSPF010000000
VERSION     DAOSPF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4198_length_14475_cov_6.255132"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2492
                     /locus_tag="PK252_12100"
     CDS             <1..2492
                     /locus_tag="PK252_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOT15494.1"
                     /translation="NTGVIDVRAHAQTPLLYYLLDASDVVLTSNGPDSIFKNLAAGTY
                     KVKVTNISHCDSATKVVVVEQDTVLNATVAYKDSACVGAVVTITPVNPYGTVTYKVTS
                     DAIGVYIKQIDDSVFTVSHTGVYHISVVDSENKRFNIDTLIKVTVPGLFAIAEKSNIS
                     CDPGSKGAFKMTVDGGTPPYSYVWLNNATGDTLSKNETDLNNVGIGKYKFVLKDNAGC
                     EFDYIDSLVFVPLIFNVTLVDKPSCITNSDTSKNGTLVVTINSGIAPFIYQWRDTVSK
                     VLLQSDTTALLTDTLKSVRSSVYQLSIFDMNGCQKDTVFTVNAADTLDVTFDLTQATC
                     AAYGQLVLKSVKGASKFKYIINDVELGSGLDNIDVTIPSLKQGFYNVTVEDGKGCQVK
                     RVMSVNLTEPISLTSIINDKTCTSLGGVSLMFSNTVYPVYASLNESTEPVSANNGELA
                     NFHDLDTGKYSLKIWNDIAGCKIDTFFNVHLNNTLQMVDTIFNQTCFKDGLIKVKVKN
                     IDNPFFYRWLNDTIKGTTSDTIFRSVKAGSYILEVIDSTFGCSKIDTFNVIQGDSLKV
                     FAGLDTTICRGVEYLLEGRAFAGKKELTVSDLVTFNWSPARNLVDNKDYIPNPTIRKY
                     IPNSYKIDSVQYKLYVTYGNCTNSDSVVISYHQSNGVWLPAQDTASKGNFEIIPSIGG
                     SGSFVSYMWLPHTYVVGGTDTARNLVVNFLGDSLTYQFMGISADGCIETASIKVIATD
                     DINPDGAFSPNGDGANEFWYIPNAEYFPDIVVKVFNRWGNLVYESKGYNNADIRWTGK
                     RNGKDMPIGTYYYIITTSGSKTKKGTVTLMR"
     gene            2523..3431
                     /locus_tag="PK252_12105"
     CDS             2523..3431
                     /locus_tag="PK252_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOT15495.1"
                     /translation="MKRLIIFSICMVAMVQSKAQQTPLYSQYMHNPFILNPAVAGTYN
                     YFQIRLNSHLQWIGFPDAPVTTSLSVFGPVSAKNKDMGYGASVYQDMTSPTSRLGLRG
                     AYAYNVALSEYIRASFSAALGFYQFKYDGTNISMRETDNVVKNGVVSEFKPDGMLGVL
                     VYSGLFQVGLSVDNLFNSKFNVNPDQENVTSLGKLTRHYYFMGSYNWRHNRRWNTEYS
                     TIVKAVEGFSVPVQMDFNVTAWYQKTAWFGASYRTLDAVSLLAGYIFNKRIYAGYSFD
                     INLSPIRYYSFGSHEVMIGYRFNTLK"
     gene            complement(3482..5056)
                     /gene="nadB"
                     /locus_tag="PK252_12110"
     CDS             complement(3482..5056)
                     /gene="nadB"
                     /locus_tag="PK252_12110"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOT15496.1"
                     /translation="MEERYDFLVIGSGAAGITFALEAANHGKVCIISKTNLEECNTKY
                     AQGGIAAVTYSPDDFEKHVKDTLICGDGLCNEEIVRLAISEAPAAIQRLIDLGTNFDK
                     DQNGKYDLAREGGHSENRILHHKDVTGAEIERALIARVRKNPNITILENHFAIDIITQ
                     HHLGMLVKRYYNNIECYGAYVLDTATGKVKTILAKATLMATGGTGNVYHTTTNPVVAT
                     GDGVAMVSRAKGIIEDMEFVQFHPTALYSPSERPEFLITEALRGFGAILKDQNGNEFM
                     QKYDPRGSLAPRDIVARAIDSEMKIAGIDYVYLDARHISYEQLIIHFPNIYKKCMSIG
                     IDMTKDYIPVAPAAHFSCGGIKVDKNGLSWIKNLYAAGEVACTGLHGANRLASNSLME
                     AVVFSHRAAIHATENCLSNSIPENIPAWNDEGTTSPEEMVLITQSMRELQQIMTNYVA
                     IVRSDLRLQRAEKRVNLIMEETQALYKKLKVSRILCELRNLTEVSYLIIKSASQRKTS
                     VGLHYNVDYPKKKSLS"
     gene            complement(5071..5874)
                     /locus_tag="PK252_12115"
     CDS             complement(5071..5874)
                     /locus_tag="PK252_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT15497.1"
                     /translation="MDTQFYIFQGKKIAYNSLGSGNTIVLLHGYLESKEIWGSFATEL
                     AKNYQIIAIDILGHGESTIDEESSDVNWMAEGIFRLLQSKQIGKAVIVGHSMGGYVML
                     AMAEAHPEIFAGAAMYHSVPNADLPEKKEGRQREIELVKQGKKQLIYNVNIPKGFADD
                     NLEKLKNEVEKAKKIASGTSDEGIIFALQAMMKRKDRTQVLFNLAVPVYFAIGKKDNY
                     IPQQRLIDLSTNVKNRRVVVFENSGHNSFIEEPELAIDEFQDFLITCKF"
     gene            complement(5923..6609)
                     /locus_tag="PK252_12120"
     CDS             complement(5923..6609)
                     /locus_tag="PK252_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT15498.1"
                     /translation="MTIITDLLDLLLPRTCCGCNTVLTTNEEYLCTKCMINLPRTRYH
                     KDPDNPTAQTFWGRTNIQNATSFLHFNKKGTVQHIMHQIKYKDNLELAIFMGTHAGHE
                     LKCTPFEEVDVIIPVPLHKSRLSKRGYNQSERIAMGISEILNIPIDNKSIIRAKSSES
                     QVTKKRYQRWENVEGIFEVVQPQQIINKHILLVDDILTTGATIEACAQEILKVEGTKI
                     SIFTLAKAKQ"
     gene            6690..8081
                     /gene="asnS"
                     /locus_tag="PK252_12125"
     CDS             6690..8081
                     /gene="asnS"
                     /locus_tag="PK252_12125"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT15499.1"
                     /translation="MSKRSIVKELLKSTVYDVDVLVKGWVRTRRGNKSVQFIALNDGS
                     TVHSIQIVVDLNTISEEALKEVTTGSCIAVNGKLIKSMGKGQTVEIQAKDLTVYGTAD
                     ADEYPLQKKGHSLEFLREIAYLRPRTNTFGCIFRLRHAMSFAIHKYFNDKGFFYFHTP
                     IITGSDCEGAGAMFRVTTLEMEKLPRGEDGKIDYSQDFFGKETSLTVSGQLEGELGAL
                     ALGGIYTFGPTFRAENSNTPRHLAEFWMIEPEVAFNDITDNMDLAEDFLKFLIKYALE
                     NCMDDLNFLADMYDKELIERLNFVINNEFKRLTYTEGVEILEKSGEKFEYPVKWGVDL
                     QSEHERYLVEKHFKKPVILTNYPKDIKAFYMKQNDDGKTVKAMDVLFPRIGEIIGGSE
                     READYNKLKARIEEMNLPMKDLWWYLNTRKYGSAPHSGFGLGFERLMLFITGMGNIRD
                     VIPFPRTPKTAEF"
     gene            8087..9544
                     /gene="rpoN"
                     /locus_tag="PK252_12130"
     CDS             8087..9544
                     /gene="rpoN"
                     /locus_tag="PK252_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOT15500.1"
                     /translation="MLKQRLEQKLQQKLTPQQIQLIKLLEIPAVSLEQRIKEELEKNP
                     MLEEEEKEEKDEVTFEEEAGSSELDSSDEFSLEDYYNEDEYIPEYKLKTNNHSVDDKR
                     VDMPAVATVSFHEFLESQLMELDLDEKQHMLASFIVGNIDDDGYIRRPLSSIADDIAF
                     LQNIQTDEIELLGILKQIQELEPVGVGARDLQECLLLQLEQKNLSLPKFQIAYKILKK
                     SFDEFSKRHYDKIKQRLNITDAELKEALDEIVKLNPKPGSIFSNATDSVIENIIPDFF
                     IELKDGNPVLLLNNRNQPELRLSKAYNDIIGSAMNGGKGNSKNKELLSFLKQKIESAK
                     WFIDAIHQRQQTLFNVMQAILNYQLEYFKTGDEKKLRPMILKDVADVTSLDISTISRV
                     VNSKYVQTHFGIFSLKSFFSESMQNEAGEEVSTREIKSILQDCVNNEDKHSPLTDDDL
                     ADILKAKGYPIARRTVAKYREQLGISVARLRKELN"
     gene            complement(9546..9839)
                     /locus_tag="PK252_12135"
     CDS             complement(9546..9839)
                     /locus_tag="PK252_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOT15501.1"
                     /translation="MTIASEKHTVELMIKLYCHSAHKSNELCPECLQLLSYAHERLEK
                     CIYGDKKTSCKKCLTHCYKKDMRNQIKKVMRYSGPRMLWHYPLLFLKHTLSPK"
     gene            complement(9845..10180)
                     /locus_tag="PK252_12140"
     CDS             complement(9845..10180)
                     /locus_tag="PK252_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT15502.1"
                     /translation="MDLNNLQPDNAKAQILSNAVAYADKSVVSKQLIKAPSGNITLFA
                     FDKGEGLSEHTAPFDAFVEILDGKAEITIGGIKHNLTTGQFIIMPANIPHALSAVEKF
                     KMMLVMIKS"
     gene            complement(10286..11941)
                     /gene="hcp"
                     /locus_tag="PK252_12145"
                     /gene_synonym="priS"
     CDS             complement(10286..11941)
                     /gene="hcp"
                     /locus_tag="PK252_12145"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOT15503.1"
                     /translation="MFCYQCQEAAKGIGCTISGVCGKNEKVANLQDLLIYSLKGLAIV
                     AQKASANGIEKKEVHDSIIDGLFVTITNANFDEKAIIERIRKVTILRDELKGTLAGKG
                     IKLEKNLFAAVYFTAGTDAELIEFSNKVSILTTENEDVRSLRELTIYGLKGMAAYAEH
                     AANLGHENTDIHKFMVKALSAVTDDNLSVNDLVGLVLETGKYGVDVMALLDKAHTSTY
                     GNPEITQVNIGVSTNPGILISGHDLKDIAELLEQTEGSGVDVYTHSEMLPAHYYPAFK
                     KYKHFVGNYGNAWWKQKEEFEKFNGPILFTTNCIVPPLENASYKDRIYTTGSAGLPGA
                     AHIGPRPKGGKKDFSAIIEHAKKCKAPIEIEKGHITGGFAHNQVGLVADKVVDAIKAG
                     TIKKFFVMAGCDGRMKSRNYYSEFAEKLPKDAVILTAGCAKYRYNKLPLGDIGGIPRV
                     LDAGQCNDSYSLVVTALKLKDAFGLESVNDLPLAYNIAWYEQKAVIVLLALLYLGVKN
                     IHLGPTLPAFLSRNVAKVLVENFGIGGISSVDEDIKKFMGDAA"
     gene            complement(12140..13036)
                     /locus_tag="PK252_12150"
     CDS             complement(12140..13036)
                     /locus_tag="PK252_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOT15504.1"
                     /translation="MVREIVQIDGAKCNGCGICAPNCHEGALQIIDGKAVLISELLCD
                     GLGACLGHCPEGAITIEKREATPYDEVSVMQEMVKKTKNVVIAHLKHLKDHNETAYLQ
                     QGVQYLIDNKKSLKFDLQEVLESLKDKKKQTPIVMQTSHAHPHQGGGCPGSRAMSFGT
                     PAPTNNPTGNIASQLTHWPIQLHLIQPAASHFIKSDLLIAADCTAFALGSFHSELLKN
                     RKLVIACPKLDTNKEVYIEKIARLIDEAQVNTITVAIMEVPCCGGLLQITQMAVNQAK
                     RKVPVKAITIGIQGDILAEEWI"
     gene            complement(13147..14088)
                     /locus_tag="PK252_12155"
     CDS             complement(13147..14088)
                     /locus_tag="PK252_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOT15505.1"
                     /translation="MLTQDTTLFAKWQPKPYIVSFETFGGNIISNATVTYNHLVNQPA
                     NPEKPGYQFDKWYKDSLFTVTWNFNSDLITENTTIYAKWIALSYNIHFDTNGGTPIED
                     TVVFYNSYINRPVNPHKNGYLFGGWFKNPNFTIIWNFENDVITANTTLYAKWISPDCT
                     VHFETTGGTTINDTVVLYNNYINRPANPHKNGYLFDGWFKDPNFTIVWNFENDVITDN
                     TTLYAKWIPVTAIHNEKKNEVIVYPVPTYNILHIKGVGFTMFELFDLSGKQVLKGRIS
                     ESSTTTAVEMSSVKPGQYAILLSSRTGEKAIFKIIKK"
     gene            complement(14024..>14475)
                     /locus_tag="PK252_12160"
     CDS             complement(14024..>14475)
                     /locus_tag="PK252_12160"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOT15506.1"
                     /translation="LTIVTFVNSLSASGDSTIETDDNTTLQEPFINNIPGYRFLGWYK
                     DTSFANQWIFATDIVTADLYLYARWTKKTFLVNFNSNGGSLVLPQNIPYQSLVNVPNM
                     PEMEHYLFSGWYVDQQLSKSWDFSSNYVNPGHNFICQMATKAIHRFF"
BASE COUNT         4059 a         2607 c         2962 g         4847 t
ORIGIN      
        1 ggaatactgg agtcattgat gtaagggctc atgctcaaac tccgttgttg tattatttgc
       61 ttgatgcttc tgacgtagtg cttacaagta atggtccgga cagtatcttc aaaaatcttg
      121 ctgccggaac ttataaagtg aaagtaacca atatttcgca ttgcgattct gctactaaag
      181 ttgttgtagt tgagcaagat actgtattga atgcaactgt ggcatataaa gattctgcat
      241 gtgttggggc agtagttact ataactcctg tcaatcctta tggcacagtt acttataaag
      301 taacatctga tgccataggt gtatatatta aacaaataga tgatagcgtg tttactgttt
      361 ctcataccgg tgtttatcat atttcggttg ttgattcgga gaacaaacgt tttaatatag
      421 atactttaat taaggttact gttcctggct tgtttgctat tgctgagaaa tctaatattt
      481 cttgcgatcc gggtagtaaa ggtgcattta aaatgactgt tgatggtggc actcctccat
      541 actcttatgt atggttgaat aatgctacag gtgatacact ttcgaaaaat gaaacagatt
      601 tgaataatgt aggaattggc aagtataagt ttgtactgaa agacaatgct ggttgtgaat
      661 ttgactacat tgacagctta gtgtttgttc ctcttatttt taatgtaaca ttagtagata
      721 agccttcatg tattaccaac tctgatactt ctaaaaatgg aaccttggtt gttaccataa
      781 attcagggat tgctccattt atctatcagt ggagggatac tgtcagcaaa gtcttgctac
      841 agtctgatac aacagccttg ttgactgata ctttgaagag tgttcgttct tctgtttatc
      901 aattatctat atttgatatg aatggatgcc agaaagatac tgtgtttact gtaaatgcag
      961 ctgatacact tgatgtaact tttgatttga cgcaagccac ctgtgctgct tatggccagc
     1021 ttgttttgaa atcagtaaaa ggtgcttcta aatttaaata tatcattaac gatgtcgagt
     1081 taggttcagg tttggacaat atagatgtta ctattccttc gctcaaacaa ggcttctata
     1141 atgttactgt tgaagatgga aagggctgcc aggtgaaacg tgtaatgtct gtgaacctga
     1201 cagaacctat tagccttact tccattatta acgataagac ttgtacaagc ttaggcggag
     1261 tgtctttaat gttctctaat actgtttatc cggtatatgc ttcgctgaat gaaagtactg
     1321 aacctgtatc tgctaataat ggtgaacttg ctaacttcca tgatttggat actggtaagt
     1381 attcattaaa aatctggaat gatatagctg gttgcaaaat tgatacattt tttaatgttc
     1441 atttgaacaa cacacttcag atggtggata caattttcaa ccaaacttgt tttaaagatg
     1501 ggctgattaa ggtgaaagta aagaatattg ataatccttt cttctatcgc tggctcaatg
     1561 ataccatcaa ggggacaaca agtgatacca tctttagaag tgttaaagcc ggaagttata
     1621 ttcttgaagt gatagatagt acctttggat gtagcaaaat agatactttt aatgtcattc
     1681 agggagattc tttgaaggta tttgcaggtt tggatactac catttgccgt ggtgttgagt
     1741 atttacttga agggcgtgct tttgctggta aaaaagagct cactgttagt gacttagtta
     1801 cctttaattg gagccctgct cgcaatttgg ttgataataa agactatatt cctaatccta
     1861 ccatccgaaa gtacattcct aattcgtata aaattgattc tgttcagtat aagctgtatg
     1921 ttacttatgg taattgtact aactctgatt cggtagtaat ttcgtatcat cagtccaatg
     1981 gtgtttggtt acctgctcag gataccgctt ctaaaggaaa ttttgaaatt attccaagta
     2041 taggtggaag tggaagtttt gtatcttaca tgtggttgcc tcatacgtat gttgtcggag
     2101 gtaccgatac agcccgcaac ttggttgtaa acttcttagg cgattcgttg acctatcagt
     2161 ttatgggtat ttctgctgat ggttgtatcg aaactgcttc tataaaagtg attgcaaccg
     2221 atgatattaa tcctgacggg gcattctcgc caaatggtga tggtgccaac gaattctggt
     2281 atatccctaa tgccgaatac ttccctgata ttgtggttaa agtatttaac cgatggggga
     2341 atttggttta cgaaagcaaa gggtataaca atgctgacat aagatggact ggtaaacgca
     2401 atggtaaaga tatgccaata ggtacttatt actacattat cacaaccagc ggttcaaaaa
     2461 ccaaaaaagg tactgttact ttaatgagat aaaagaaagc taaaatgaaa tatctgaaat
     2521 atatgaaacg gttaatcatt ttctcaatat gcatggttgc tatggtccaa tccaaagcac
     2581 aacagacacc gctttattcg cagtacatgc ataacccgtt tattctgaat cctgctgtgg
     2641 caggaactta taattatttt caaatcaggt taaattcgca tttgcagtgg attggttttc
     2701 cagatgcccc ggtaacaaca tcattgagcg tttttggccc ggtaagtgcc aagaacaaag
     2761 atatgggcta tggagcttca gtatatcagg atatgacttc tcctacaagc cgtctggggt
     2821 tgaggggagc ttatgcgtac aacgttgctt tgtcagagta tataagggca tctttttctg
     2881 ctgctttggg tttttaccag tttaagtatg acggtaccaa tatatccatg cgtgaaacag
     2941 acaacgtggt aaaaaatggt gttgtttctg aatttaaacc tgatggaatg ttaggcgttt
     3001 tggtttattc aggtttattc caggttgggc tttctgttga caaccttttc aacagtaagt
     3061 ttaacgttaa tcctgatcaa gaaaatgtta caagtttagg aaagcttacc cgtcattatt
     3121 attttatggg atcatataat tggcgtcaca accgcagatg gaatactgaa tatagtacaa
     3181 tagtaaaagc agtagaaggg tttagtgtgc cagtgcaaat ggatttcaat gttactgcat
     3241 ggtatcagaa aactgcctgg tttggggctt catacagaac tttggacgct gtatctttat
     3301 tagcaggtta tatttttaac aagcgtattt atgccggata ttcatttgat attaaccttt
     3361 caccaatacg ttattatagc tttggaagcc acgaagtaat gattggttac aggtttaata
     3421 ccttgaagta aaaaatcaaa agtaattggg taaattactt gaaatcgttg ggttaactgg
     3481 tttaactcaa cgatttcttt tttggatagt ccacattgta atgcaatcca acactggttt
     3541 tacgttgtga tgcactcttt ataataaggt aactcacttc tgtcaggtta cgtaattcgc
     3601 aaaggatgcg tgatactttc agttttttat aaagagcctg tgtttcttcc atgataaggt
     3661 ttacacgttt ttcggccctc tgcaacctaa ggtcgctgcg gacaatggcc acatagttgg
     3721 tcataatttg ttgcagttcc ctcatactct gggtgataag aaccatttct tcgggcgagg
     3781 tggtaccttc atcgttccag gcagggatat tttcaggaat agaattgctt aggcaatttt
     3841 cagtagcatg aatagctgcc ctgtgggaaa aaacaacagc ttccatcaat gaattagaag
     3901 caaggcggtt ggcgccatgt aagcctgtgc aggctacttc tccggcagca taaaggtttt
     3961 taatccacga aagcccgttt ttgtccactt ttattcctcc acaagagaag tgagcagccg
     4021 gggcaacggg tatataatct tttgtcatgt caatgccaat actcatacac tttttgtaaa
     4081 tgttagggaa gtgtataatt agctgttcat agcttatatg acgtgcatca aggtaaacat
     4141 agtcaatacc ggctattttc atttcgctgt cgattgcgcg ggctacaata tctctgggtg
     4201 caagagagcc acgagggtca tatttttgca tgaattcatt gccattctgg tctttgagga
     4261 tggcgccaaa acctctgagc gcttctgtaa taagaaattc aggcctttca ctgggcgaat
     4321 ataatgcagt agggtggaac tggacaaatt ccatatcttc aataatgcct tttgcgcgcg
     4381 aaaccattgc tacaccatcg cctgtagcta caacagggtt tgtggtggtg tgatatacat
     4441 tgccggtacc cccggttgcc attaatgtgg cttttgccag tattgttttt accttacctg
     4501 tagctgtatc taatacgtaa gccccataac attcaatatt attgtaatat cttttgacta
     4561 acattcccaa atggtgttgg gtaataatat caatggcaaa atgattttcg aggatggtaa
     4621 tattcgggtt ttttcggact cttgcaatta aagccctttc aatttcagct cctgtgacat
     4681 ctttgtgatg cagtatcctg ttctcgctgt gtccaccttc acgggcaagg tcgtatttgc
     4741 cgttctggtc tttgtcaaag ttggttccaa ggtcaatcaa acgctgtatt gcagcaggag
     4801 cttcactaat ggcaaggcgt actatttctt cgttacatag tccgtctccg caaatcaggg
     4861 tgtctttaac gtgtttttca aaatcatcgg gcgaatatgt aactgcagca attccgccct
     4921 gggcatattt agtattgcac tcctcaaggt ttgttttgct tataatacat acttttccat
     4981 ggttagcagc ttccaacgca aatgttatcc cggcagctcc gctaccaata accagaaaat
     5041 catacctttc ttccattagt gataatgttg ttaaaattta catgttatta aaaagtcctg
     5101 aaactcgtct atggcaagtt caggctcttc aatgaaacta ttatggccgc tgttttcaaa
     5161 tacaacaaca cgcctgtttt tgacatttgt gctcaaatca ataagtcgtt gctgaggaat
     5221 ataattgtct tttttcccaa tggcaaaata taccggaaca gcgaggttga aaagtacctg
     5281 agtgcggtct ttacgtttca tcatggcctg caatgcaaaa atgatgcctt cgtcagaagt
     5341 gcccgaagct attttttttg ctttttctac ttcgtttttc aatttttcga ggttatcatc
     5401 tgcaaatcct ttaggaatat tgacgttata tatgagttgc tttttgcctt gttttaccag
     5461 ctcaatttct cgctggcggc cctctttttt ctcaggcaaa tcggcgttag gaacagaatg
     5521 gtacattgcc gccccggcaa atatttctgg atgggcttct gccattgcta acataacata
     5581 tcctcccata gaatgcccta caataacagc tttgccaatt tgttttgatt gtaaaagccg
     5641 gaatattcct tcagccatcc aatttacatc tgaagattct tcatctatgg tactttcgcc
     5701 atgccctaat atgtcaatag ctataatctg atagtttttg gcaagttctg ttgcaaacga
     5761 gccccaaatt tcttttgatt cgagataacc atgtaataaa actattgtgt ttcctgagcc
     5821 taatgaattg taggcaatct tttttccctg gaatatgtaa aattgtgtgt ccatattttt
     5881 tgtgcaaagg tattttaaag taagataatt gttttaaaaa tattattgtt ttgcttttgc
     5941 cagtgtaaat atgcttattt tcgtcccttc tacttttaat atttcctggg cacaggcttc
     6001 tattgtagct cctgtggtga gtatatcgtc aacaagcagg atgtgtttgt tgataatttg
     6061 ttgtggttgt accacttcaa aaatgccttc tacattttcc cagcgttgat atctcttttt
     6121 tgtcacctgg ctttcacttg acttggcgcg gataatactt ttattgtcaa tcggtatatt
     6181 taaaatttct gatattccca ttgcaatacg ctcgctttgg ttgtatcctc tttttgaaag
     6241 tctcgatttg tgcaaaggaa ctgggataat aacgtcaact tcttcaaatg gtgtgcattt
     6301 taattcatgt cccgcatgag tccccataaa tattgcaagc tccaggttgt ctttgtattt
     6361 tatctgatgc attatgtgtt gtactgtgcc ttttttgttg aaatgtagaa aagatgtagc
     6421 gttttgaatg tttgtccgcc cccagaacgt ttgggcggta ggattgtcag ggtctttgtg
     6481 atatcgggta cgagggaggt ttatcatgca ttttgtgcat aaatattctt cgtttgttgt
     6541 tagtactgtg ttgcagccgc aacatgtgcg aggtaaaagc aagtcgagaa ggtctgttat
     6601 tattgtcatt gaattatttt accagagggg tttagtctta taaaaatatt aaatttgcaa
     6661 acattattca ataggctaaa ggatttatta tgtcaaaaag aagtatagtt aaggagttgc
     6721 tgaaatctac agtgtatgat gtagatgtgc tggtaaaagg ctgggtgagg acccgcagag
     6781 gaaataaaag tgttcagttt attgctctta atgatggttc tacagttcat tcaattcaaa
     6841 ttgtagtaga tttaaatact atttcagaag aagctttgaa ggaagttact actggttctt
     6901 gtattgctgt taatggtaaa ctcattaaat cgatgggtaa aggccagacg gtagagatac
     6961 aagctaaaga ccttactgta tatggtacag ctgatgctga tgaatatcct ttgcaaaaga
     7021 aagggcactc tttagagttc ttgcgtgaga ttgcttattt acgcccgcgc accaatactt
     7081 ttggctgtat tttccgctta cgacatgcta tgtcctttgc tattcataaa tattttaatg
     7141 acaaaggttt tttttatttt cataccccta ttataacagg ttctgactgt gagggcgccg
     7201 gggcaatgtt tagggttact actttggaga tggaaaaatt acctcgggga gaagatggga
     7261 agattgatta ttctcaagat ttctttggta aggaaaccag cctgactgtt tcaggacagc
     7321 tcgaaggtga gcttggtgcc cttgctcttg gtggtattta tacttttggg ccaactttca
     7381 gggctgaaaa ttccaatact ccgcgtcatc ttgctgagtt ttggatgatt gagcctgagg
     7441 ttgcttttaa tgatattact gataatatgg atttggctga ggatttccta aaattcctta
     7501 tcaaatatgc gttggaaaac tgtatggatg atcttaattt tctggccgat atgtacgata
     7561 aagaactgat cgaacgttta aattttgtta taaacaatga atttaagcgt cttacatata
     7621 cagaaggcgt tgaaatcctt gaaaaatctg gtgaaaagtt tgagtatcct gtaaagtggg
     7681 gtgttgacct tcaatctgag catgaaagat atttggtaga gaaacatttc aaaaaaccgg
     7741 ttatcctaac caattatcct aaagatataa aagcttttta tatgaagcag aacgatgatg
     7801 gcaaaacagt taaggcgatg gatgttttgt ttccaagaat tggcgaaatt attggaggat
     7861 cagagcgtga agctgattat aataagctaa aagcccgaat tgaagaaatg aaccttccta
     7921 tgaaagacct ttggtggtat ttaaataccc gtaaatatgg aagtgcgccc catagtggct
     7981 tcggacttgg ttttgagcgc ctgatgcttt tcattacggg tatgggtaat atccgtgatg
     8041 ttattccttt tcctcgtact cctaaaactg ctgagtttta aatccaatgt taaagcagcg
     8101 attagaacag aaacttcaac aaaagcttac tccgcaacag atacaactta tcaagttgct
     8161 tgaaatccct gcggtttctc ttgaacaacg cataaaagag gagttggaga aaaatcctat
     8221 gcttgaagag gaagaaaagg aagaaaaaga tgaggtgact tttgaagaag aggccggtag
     8281 ttctgaactt gacagtagtg atgagttttc cttagaagat tattacaacg aagacgaata
     8341 tatcccagaa tataagctta aaaccaacaa tcattctgta gatgacaaaa gggttgatat
     8401 gcctgccgtt gctactgtat cgtttcacga atttttagaa tcgcagctaa tggagctcga
     8461 tttggacgaa aaacagcaca tgctggcttc ttttattgtt ggtaatattg atgatgatgg
     8521 ctatattcgc aggcctttgt cttcaattgc tgatgatatc gcttttttgc agaatattca
     8581 aacggatgag attgagctgt tgggtatttt aaaacaaatt caggagcttg agcctgttgg
     8641 tgttggtgcc cgggatttgc aggaatgcct tttgcttcag cttgagcaaa aaaatcttag
     8701 tcttcccaaa tttcagatag cgtataaaat tctgaaaaaa tcttttgatg aattttcaaa
     8761 gcgtcattac gataaaatca aacaacgcct caatattaca gatgcagaac ttaaagaagc
     8821 gttggatgag attgtaaaac ttaaccctaa accgggtagc atattttcaa atgcaaccga
     8881 cagcgtaatt gagaatataa ttcccgattt cttcattgaa ttgaaagacg ggaaccctgt
     8941 attgctttta aataaccgaa atcagcctga actacgtttg agtaaagcct ataatgatat
     9001 tattggctct gccatgaatg gcggcaaagg caattcaaag aataaggagc tgttgagctt
     9061 tctaaagcag aaaattgaat cggccaaatg gtttattgac gcaatacatc agcgacaaca
     9121 aacattgttt aatgttatgc aggctatttt gaattatcag ttagaatatt ttaagactgg
     9181 agatgagaaa aagcttaggc caatgatact taaagatgta gctgatgtta cttctctcga
     9241 tatatctact atttcacgag tggtaaatag caagtatgtg caaacgcatt ttgggatctt
     9301 ttctttaaaa tctttctttt ctgagtcgat gcaaaatgaa gcaggggagg aggtttctac
     9361 ccgtgaaatc aagtctattc ttcaggattg tgttaataat gaagataaac attctccttt
     9421 aaccgatgac gatttggccg acatcctgaa agccaagggc tatcctattg cccgtcgtac
     9481 cgtggccaaa tatcgcgaac agcttggcat ttctgtagcc aggttgcgga aagagttaaa
     9541 ttgatttatt ttggactaag cgtatgcttc agaaataaca agggataatg ccataacatt
     9601 cgtggtcctg aataacgcat aactttcttt atctggttac gcatatcctt tttgtagcaa
     9661 tgggttagac attttttgca tgatgtcttt ttgtcaccat agatacactt ttccagccgt
     9721 tcatgtgcgt atgacaataa ctgtaaacat tcggggcaca gttcattgga cttgtgcgct
     9781 gaatggcaat acagctttat catcaactca acggtatgtt tctcgctggc tattgtcata
     9841 gtatttatga cttaatcatt accagcatca tcttaaactt ttcaacagcg cttaaagcat
     9901 gaggaatatt agctggcatg attataaatt gtcccgttgt aagattgtgt tttattccac
     9961 caattgttat ttcagctttc ccgtcaagta tctctacaaa agcatcaaac ggagcggtgt
    10021 gttcgctaag cccttcgcct ttgtcaaaag caaacagggt gatattgcct gatggggctt
    10081 ttataagttg cttactaact acacttttgt ctgcataagc tactgcattg cttaatattt
    10141 gagcttttgc attgtccggt tgtaaattat ttaagtccat attattaaaa ttaggattaa
    10201 taaacgcaaa gacgctaagg cgctatgatc tttacttcgt gcttttgagc cttagcgttt
    10261 ataatataaa aatacttcaa aataattagg cagcgtcgcc catgaacttt ttgatatctt
    10321 catcaacact ggatattcct ccaataccga agttttcaac cagtacttta gccacgttac
    10381 gagacaaaaa ggctggcaat gttgggccga ggtggatgtt tttcacccca aggtaaagta
    10441 aagccaacaa tacaataaca gctttctgct cgtaccaggc aatgttgtaa gccaatggta
    10501 agtcgttaac actttccaat ccaaatgcat ctttcagttt tagtgccgtt acaaccagtg
    10561 aataagagtc gttgcactgg ccagcatcga gtacacgagg aatgccgcct atgtcgccaa
    10621 gtggaagttt gttgtacctg tatttggcgc atcctgctgt taagataaca gcatcttttg
    10681 ggagtttttc ggcaaattcg ctgtagtagt tccttgattt catgcgtcca tcacagcctg
    10741 ccatgacaaa aaacttttta atagtgccgg ctttaatggc atctactacc ttatcggcta
    10801 ccaagcctac ctggttatgt gcaaagccgc ctgtgatgtg ccctttttca atctcaatcg
    10861 gggctttaca tttcttggca tgttcaatga tggctgaaaa atctttcttt ccgccttttg
    10921 gtcgtggccc gatatgtgca gctcccggaa gccctgcaga gcctgtagta taaattctgt
    10981 ctttgtagct ggcgttctca agcggaggta caatgcagtt ggtagtgaat aaaatcgggc
    11041 cattgaactt ttcaaattcc tctttttgct tccaccaggc gtttccgtag ttgccaacaa
    11101 aatgtttgta tttcttgaat gccgggtagt agtgcgcagg aagcatttca ctgtgggtgt
    11161 aaacgtcaac tccgcttcct tctgtctgtt caagcaattc agcaatgtct ttcaggtcgt
    11221 ggccgctgat taagatgcca gggttagtgc taacgccaat gtttacctgt gttatttcag
    11281 gatttccata agttgaggtg tgtgctttat ctaaaagagc cattacatct acgccgtatt
    11341 tgcctgtttc aagtacaagg ccgaccaaat cgttcacaga caagttgtca tctgttacgg
    11401 cagataatgc tttaaccata aacttgtgaa tgtctgtatt ttcgtggcca aggtttgctg
    11461 catgttctgc ataggcagcc atgcctttta atccgtagat ggtcaattca cgtaatgagc
    11521 gtacatcttc gttttctgta gttaatatgc tgactttgtt tgaaaattca atgagctcag
    11581 catcagtacc ggcagtgaaa taaactgcag caaacaagtt tttttccagt tttattcctt
    11641 tcccggcaag ggttcctttt agttcgtccc tgagtatggt tactttacgg atacgttcga
    11701 taatagcttt ttcgtcaaag ttagcattgg taatggttac aaacagcccg tcaatgatgc
    11761 tgtcgtgtac ttcttttttc tcaattccat tggcagatgc tttttgagcc actattgcca
    11821 agcctttgag cgaataaata agcaggtctt gcaagttggc aactttttca tttttgccgc
    11881 atacaccact tattgtgcag cctatgcctt tagctgcttc ctgacattga taacaaaaca
    11941 ttcccatttt tgtatttttt aaatttatta taaagattaa taataagtac ttaaatgctt
    12001 taaattttta ttaaacttgt tatttatttt atctaaaaaa ttgttggtta ttgtatttct
    12061 ttttatcgat tactttagaa aaatgttcaa cgagttatat ttaccatcaa ctaacaacta
    12121 acaactaaca accatcctcc tatatccatt cttcagccaa tatatcgcct tgaatcccta
    12181 tggtaatagc ctttactggt acttttcttt ttgcctggtt gacagccatt tgtgttattt
    12241 gtagcaatcc tccacagcag ggaacttcca taattgctac agttatagtg tttacctgtg
    12301 cttcgtcaat taggcgggct attttttcga tataaacttc tttatttgtg tccagctttg
    12361 gacaagcaat cacgagtttt cggtttttca gcagttcgct gtggaaggag cctaatgcaa
    12421 aggcagtaca atcggctgct ataagcaaat cgcttttgat aaagtgcgat gctgcgggtt
    12481 gtataaggtg cagctgaata ggccaatggg taagctgtga agcaatattg cctgttggat
    12541 tatttgttgg ggctggcgtt ccaaatgaca tagcccttga acccggacat cctccaccct
    12601 gatgagggtg tgcatgtgat gtttgcataa ctattggtgt ttgttttttt ttatccttaa
    12661 gactttctaa tacttcttgt aaatcaaact tcaggctttt tttattgtca attaagtact
    12721 ggaccccttg ttgcagataa gctgtctcat tgtggtcttt gagatgcttc aagtgggcaa
    12781 tgacaacatt ctttgttttt ttgaccattt cctgcatgac tgaaacttca tcgtaaggtg
    12841 tggcttcccg tttttcaatg gttatagcgc cttcaggaca atgtccgaga caagcgccaa
    12901 gtccatcaca tagcaattcg cttatcagaa cggcttttcc atctatgatt tgtaaagcac
    12961 cttcgtggca attgggtgca caaataccac aaccattgca cttagctcca tctatttgaa
    13021 caatttctct gaccatatat ttttttattg atgatgcaaa tataatacaa ataattaaat
    13081 aagtacttta atacttaaat atttttatga ataataaata tttcttattt aaaactttgt
    13141 aaccgtttat ttttttatga ttttaaatat ggctttttcg ccagtcctac tgcttaatag
    13201 tatagcgtat tgcccaggtt tgacagagct catctcaaca gcagttgttg tggagctttc
    13261 ggatattctg ccttttaata cttgttttcc tgataagtca aataattcaa acattgtaaa
    13321 gcctacacct ttgatatgta atatattgta ggtgggtact ggataaacaa tgacttcatt
    13381 ctttttttcg ttatgaatag ctgtaacagg aatccatttt gcataaaggg ttgtattatc
    13441 agtaattaca tcattttcaa aattccagac aatggtaaaa tttgggtctt tgaaccagcc
    13501 atcaaaaagg tacccgtttt tatgcggatt agctgggcga ttgatatagt tgttgtataa
    13561 aacaacagtg tcgttaatag ttgtgccgcc tgtagtttca aagtgtaccg tacaatcagg
    13621 tgaaatccat tttgcataaa gggtcgtgtt agctgtaatt acatcatttt caaaattcca
    13681 aataatggta aaatttgggt ttttgaacca gccaccaaac aggtacccat ttttatgcgg
    13741 attgacaggg cgattgatat agctgttgta aaaaacaact gtatcttcaa taggcgtgcc
    13801 gccattagtg tcaaagtgga tgttatagct gagggcaatc catttagcgt aaatggtagt
    13861 attttctgtt atcaaatcag agttaaagtt ccaggtaaca gtaaataaag aatctttgta
    13921 ccatttgtca aactgatagc ctggtttttc agggttagca ggttggttta ccagatgatt
    13981 gtaggtaaca gttgcattgc ttataatatt gcccccaaat gtttcaaaag aaacgatgta
    14041 tggctttggt tgccatttgg caaataaagt tgtgtcctgg gttaacatag tttgacgaaa
    14101 agtcccagct tttggaaagc tgttggtcaa cataccatcc tgaaaacagg tagtgttcca
    14161 tttctggcat gttggggaca tttaccaatg actggtatgg gatattctgt ggtaacacaa
    14221 ggcttcctcc gttggagttg aaattgacta aaaaagtttt tttggtccaa cgggcatata
    14281 aataaaggtc agcagttaca atgtcagtgg caaaaatcca ttggttggca aagcttgtgt
    14341 ctttatacca gcccagaaag cgatatccgg ggatattgtt aatgaaaggt tcttgaagag
    14401 tggtattgtc atcagtttca attgttgaat cacctgaagc cgacagacta ttgacaaagg
    14461 taacaatggt aagtc
//
LOCUS       DAOSPF010000073        14403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4227_length_14403_cov_7.512057, whole genome shotgun sequence.
ACCESSION   DAOSPF010000073 DAOSPF010000000
VERSION     DAOSPF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_14403_cov_7.512057"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1158)
                     /locus_tag="PK252_12165"
     CDS             complement(217..1158)
                     /locus_tag="PK252_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15507.1"
                     /translation="MNIAVLDLGTNTFNLLIAQWQNMTLQTIENNKIAVKIGKGGIHK
                     NQITEDAAERALNAIKLHKTTIDKYSVEKIIAIATSAFRTAKNASEIIDKINKQTGID
                     IEVISGEREAELIYLGVRQTLDIKDDNFLIVDIGGGSNELIIANSHKIIWKESYPLGM
                     ARLIERFQFSDPIKNSEIDQLNSYFDETMQSFSKILKKLEVKHLAGAEGAFESFINIT
                     KANNLGTLPSNNYTNKAITIDVSQFYQVQQLLQKSTLSERLSLPGLEPFRAEMIVPAG
                     IFINYIVEKTKSQAISISPFSLKEGVAWDFFNNLNSI"
     gene            complement(1165..2424)
                     /locus_tag="PK252_12170"
     CDS             complement(1165..2424)
                     /locus_tag="PK252_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOT15508.1"
                     /translation="MFKFFKDLKNKAHTPVFSGLEILKYIGPGLLVTVGFIDPGNWAS
                     NIAAGANFGYTLLWMVTLATIMLIILQHNVAHLGIATGLCLSEAANKHLPGYISRPII
                     GSAMLASVSTSLAEILGGALALNMLFKLPVKIGAVLVTALIVFLLLSNTYRKLEKWII
                     GFVSIIGISFIYELVIADVSWNEAVIGWIKPQIPTNAIVIVMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLQNETVIKKQLKYEFTDTFISMLIGWAINSAMIIMAAATFYQQKIPVTE
                     LGQAQEMLSPILGPAAGLIFAIALLMSGIASTITSGMAGGSIFAGFFGEPLDLKDNHS
                     RLGVYISLFAALALIFFISDPFKGLIYSQMILSIQLPFTIFLQIYLTSSKKVMGKYAN
                     STWTKFVLLSIGIVVSYLNIRLLMSFL"
     gene            complement(2517..5324)
                     /locus_tag="PK252_12175"
     CDS             complement(2517..5324)
                     /locus_tag="PK252_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT15509.1"
                     /translation="MKNKILLFAFISQIAFAVISGQNFDSNALIPTDSSIVIKTLPNG
                     LKYYIKKNVRPEKRMDIRLVVNAGSILEDSDQIGLAHFTEHMAFNGTKHFEKNKLVSY
                     LQSMGIKFGPEINAYTGFDETVYILQLPTNNDTLKEKGFEILSDWAQNITFDSTEIEK
                     ERGVIIEEWRLGRGAEQRMMDKYFPILFYKSKYANRLPIGTKESLESFKRSQITRFYN
                     RWYRPNNMAVIAVGDFDVKEIESLIIKYFSGMTSPQMTEERPEFGLLPHKEMLVSVNT
                     DKEANETVVNLYYKLPFKQELTIGDYQQGIKRMLYFQMLNGRFDERLRKPNPPCIYAG
                     ATMSRLGRKNNAYILSALVSEDGISKGLQALIEENTRVRKFGFTASELDRAKSEILNL
                     FEKYYNERDKSESEQFSEEYARNFLTKESIPGIVFEYNLVKHTLPQISLEEINNLPSK
                     WLIDSNRVIIVTAPEKEGLAIPSKDSLIAIVNKTETSDIKPYKDEFKNEKLIDKELKA
                     QKISKESKNKETGIATIEFANGIKINFKQTDFKDNEILFSAISKGGQSKYPDKDMYSA
                     FFSASIIEESGLGKFSADDLRKMLAGKSVSVSPYINQYTEGIKASCTPKDMETMFQLI
                     YLYFTTPRIDTLAYQSWRTRIKSYLVNVMSDPMVYYSDQVSKILSQNNPRGSAVPKPE
                     DIDKIDLNKVLNIYKERFADANDFAYTICGNFNVDSLKKYASIYLANLKVIKGSENYT
                     DLGIRPPKGLTVKDIYKGSDPKSMVTVVIDDSISYNAKQAYYLRSLEDYLDIRLYEVL
                     REEMSGVYGVGASCSMSRIPYQHYTITFKIPCSPANTDSLVKEAFHIVDLVKQNGIDS
                     SYMVKIKETQLRELEVSEKINSFWLSYIENSMLYGDDIKRILNKKADIEKLSSSDIQH
                     ITKRVFKPEYIRVTLYPEKKE"
     gene            complement(5422..7455)
                     /locus_tag="PK252_12180"
     CDS             complement(5422..7455)
                     /locus_tag="PK252_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT15510.1"
                     /translation="MKILALLVIFLPLTGTYALGQTAYDKATFDDAEYFFVQEDYTEA
                     ISSYLKLYKKNFKNNPNINYKIGVCYLNIAGQKEKSIPFLEYAALNITNNYKEGSIKE
                     TKAPIDVLLFLGNAYRINMQYEKAIEAYNKFLALTSNPKELQMEKEWARNQIEACQRA
                     KAATNSIVRIKISSLGKPINTKEDNINPAISANEKRMVYITKQKFYYALMFSEKNKSN
                     KWGLPLNITGNIMSDGNQFPLYLSPDGNTLLLNYIDGENSDIYMAKFEKKKWQPSQPL
                     SKAINSRYWESNACLSPGGDTIFFSSNRPESVGGLDIFYSIKTSTGEWGPAVNIGNTI
                     NTKLNEDCPYLSYSGDTLYFASQGHESMGGFDIFYSVKDSNGNWSAPINIGYPVNTTD
                     DDLFFIPVANSYTAYQSRYLKTGGMGNFDICKIEFFNHGRPFPFTIKGNVNYLLQISK
                     AENYNILLTNDSGKVISDTIKANADGSFEFSKAADTYRVLFIKDTSKVISNRFVIPEN
                     YPSDTYTLTTEIIQDKIAYSSFINSIEEPNKPILIRNILFSFNDVKINDDDKKEIVTI
                     ANMLKKYPEMQIRLIGHTDALGSEEYNQKLSEKRAGVVKNVLLALKVDEKQIITEGRS
                     LNEPIAINANTDGSDNPVGRAFNRRVEFELVNCPDARLKIDPIKVPAELKAQK"
     gene            complement(7626..8132)
                     /locus_tag="PK252_12185"
     CDS             complement(7626..8132)
                     /locus_tag="PK252_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT15511.1"
                     /translation="MTAIEFQHKLVGLEGSLERFAYSLTLNREDAKDLLQETFLKALT
                     YKDKFVQNDNFKAWTYTIMKNTFINNYRRNIRQNTHRDNTKEGYFLNHMSVTGNDDPD
                     SSYSTEELTKTINELEDEFRIPFQMHHDGYKYKEIADELNLNIGTVKSRIFFSRKKLT
                     EKLKDFAA"
     gene            8481..10586
                     /locus_tag="PK252_12190"
     CDS             8481..10586
                     /locus_tag="PK252_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15512.1"
                     /translation="MHLFLKYLFILFALLWYGMFNVYAQHEGSGIPFSRNTIAEDAFP
                     LIELMPIDSSEIVAAKSQKIAVNAIVREIDIRTASEGAWYKIKDRNVWVARLKSAYAQ
                     SLSFMIENFFLKNGEKLFIYNAYEVLGAYTKASNRANRKFATRQIKGDNVIIEFSTPY
                     PVAESGTFTFTSVFHSFSVDEYTSCNINASCIEGDNWKKERRAICKINVGARNCTGVL
                     INNTLKDETPYVLTANHCIWDNTEAENSVFTFNYETSACNSQIAYAGHVLSGATVMCG
                     RYENDYTLLKLMDHPPVSFMPYYAGWSLRSDENLDTVTVLHHAGGTPLKISQSVSRPL
                     DSTFKEDDEPAYANGAFWLVQKYNFGITETGSSGAPLFDKEHLIVGTLSGGDASNLQC
                     KNDLNDYYQMFNKSYTVQPLTGLPMINYLNPNNAPVASLAGFDPLEKNVYGCDTFSNI
                     ADTAGRVLHLYPYGNGYLAGHNEDSISQFGEVFENADSIVLYGAWFHVAKCGTNGNIV
                     VRAFNGSDFSSSAVFEKVISQSQMVKDKYHYIEFYPGINLTGNYTITFQVEYQHSDTF
                     AVYMSALNNEAKVSSAIINNNGWQNMTDFLPGHESVSFDISAMYCKEKNKTAINKAEI
                     SQLKVFPNPAKDWVVVNIEGLTNPEVQLFNIYGQVVNISLSINNSFIKFNVSNLKSGI
                     YLIKISCKNRLIIGKFLKV"
     gene            10762..14139
                     /locus_tag="PK252_12195"
     CDS             10762..14139
                     /locus_tag="PK252_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15513.1"
                     /translation="MMKLKIWLCLLPVIFYSINFLGQGKKKVSITTAIEVQNEDGEGL
                     SGVSAKLFNKGQEEKSVVTTNSGIVTFTLEPNSDYLIELSKDGFVSKIISVNTKVPSY
                     EDQQFEVAFPVLLFQPCEGLDYSILQKPVVKLVHNEKKRDFLPEKVYDEVMQEKLSKL
                     YEDNQNCLDEKYNAIVREGDRLFKSKKYEEAVSPYQKALGIRPNDKYVKSQLDAIDKL
                     LAESKDKEATYKKYIEQADKQMADKNYPLAKEFYNKSLGVKPGSAYPTSQIAEIDKIL
                     AQQKQDETNKQQLQQQVTSLVAQGDGSDDYCGAVLKAYKDALALKPNDPAILAKISAA
                     EKKCNDIKAAIANDKKKQEQYKASIAKADSLYKANKYDAAIAEYQNASKIIPAEKYPK
                     DQVDLINDIVKKQSKDRDAQFKALVAQGDKSEDYCGTVQNSYREALKLKPNDPELLTK
                     ISIAEKKCNDANAQQADLKKRQAGYKASVAKADSLYKANKYDAAIAEYQNAMKFVPSE
                     KYPQDQIDLINEMVRKQMKDAESQYKSLIKEADKAFDNERFDFAKDSYLKASKIKPNE
                     AYPNDKIKEIDNIITAQKVAEAKRKEIEEKYKQKIAEADAAMKNNNYMMAKNLYNEAL
                     TIKLEKYPSDRIEEIDQLMKAQADKAEKEYQSKVKSADKNFVDSKLKEALADYQAALA
                     MKPNETYPQQRIDAINKMLEEKAKKEAEEKAKKDAYASAIARADKLLAENNFDQAAAA
                     YKEASSYQPSEQYPYAKIDEIAKLKKQKETEASYAKAIITGDDYFNKKQLQQAKSFYN
                     QALGFKPGDKSATDKIANVDRLINEDIKRLADEKARKDAYDKAIIDADKSLALKDYDM
                     AKASYQTALSIDANQAYPRQKIAEVDKLIKEKEINDNYQSALDEAQKLFDAKSFDMAK
                     TKYREAKKIKPSETFPDTKVKEIEAAQAQMEKDRLQKEKKEKDYKASVQLAESLFENA
                     RYDEAQKEYEKALLILPDEVYPKQKIAKIKEIKMLLEKEAKASKTAAAPAPKKAEPVK
                     GKLDKLVFANESERAQFLSDLLTSYPPGITHEVYKDKIKTVNRYIIIRDNQANDFRSI
                     RLSYGMEYYKNDKPITEQYFNSQVKVRAGEYYNKVDL"
BASE COUNT         4599 a         2418 c         2682 g         4704 t
ORIGIN      
        1 aaaagaaaaa ttccttttca tcgccttttt gttcgatttc ggccgagaag cctaatgctc
       61 ctgttgtttg catattgcaa cattacaagg cttcgagggt gaaagagtta aggcgataag
      121 agtttttgag caactttttt cgataaaaaa gttgcataga gacatagtat ttattgctaa
      181 gtcaatattt ttaagtaata atttacaatg ttattattat atagaattca ggttattaaa
      241 gaaatcccag gcaacccctt cttttaatga aaaaggagaa atggatattg cctgggattt
      301 tgttttttca acaatatagt tgatgaaaat cccggcaggc actatcattt ctgccctgaa
      361 tggttctaat cccggcaatg atagcctttc tgataaagtc gatttttgta gtagctgttg
      421 aacttggtaa aattgcgata catcaatcgt tatggctttg ttagtatagt tgttagaagg
      481 tagagttcct aaattgtttg cttttgtgat atttatgaaa gattcaaatg caccttcagc
      541 tccagcaagg tgttttacct caagcttttt taatattttg ctgaaagatt gcatggtctc
      601 atcaaagtag ctgtttaatt gatctatctc agagtttttt atagggtctg aaaattggaa
      661 acgttcaatt aacctggcca ttccaagagg ataactttct ttccatatta ttttatggct
      721 gtttgctata ataagttcat tacttcctcc gccaatatca acaattagga agttgtcatc
      781 tttaatatcc aatgtttgac gtactcccag atatatcagt tcagcttctc tttctccaga
      841 aataacttca atgtcaatac ctgtttgttt attaatttta tcaattattt cagaggcatt
      901 tttagctgtt ctaaaagcag aggtagctat agcaattatt ttttcaacac tatacttgtc
      961 aattgtagtt ttatgaagct ttatggcgtt taaagctctt tctgcagcgt cttcggtaat
     1021 ttgattttta tgaatgcctc ctttaccaat ttttacagct attttattgt tttcaattgt
     1081 ttgaagggtc atattttgcc attgagcaat gagtaaattg aaggtattgg taccaaggtc
     1141 gagtactgca atattcatat tcttttataa aaagctcatt aaaagacgga tgttgaggta
     1201 acttacaact atgccaatgg acagtaaaac aaacttagtc caggtagaat tagcatattt
     1261 tcccatgact ttcttagatg aagtgaggta aatctgaaga aaaatggtga aaggcagttg
     1321 tatgctcaat atcatttgcg aataaatcaa acccttgaaa gggtctgaaa taaagaagat
     1381 taatgctaat gctgcaaata gtgatatata tacccctaag cggctatggt tgtcttttaa
     1441 atccaacggt tcgccaaaga aacctgcaaa tattgaacct ccagccattc ccgaagtaat
     1501 agttgatgca atgccagaca ttaatagtgc aatagcgaaa attaaccctg cggcaggtcc
     1561 taaaatggga gaaagcattt cctgtgcctg tccaagttct gttacgggta ttttttgctg
     1621 gtaaaatgtt gcagcagcca tgataatcat ggcgctgttg atagcccagc caatcaacat
     1681 agatataaat gtatcggtaa attcatattt tagttgtttc tttattaccg tttcattttg
     1741 caaattccat tgacggcttt gaattacctc gctatgcaaa aaaaggttgt gtggcattac
     1801 tactgcacct aatacgctca tcactatgac aatggcattg gtcggtattt gcggttttat
     1861 ccagcctatt acagcttcat tccagctaac atcagctatg acaagttcgt aaataaatga
     1921 aatgcctata atagatacaa acccgatgat ccatttttct aatttgcgat aggtattgga
     1981 caataataga aagacaatca gggcggttac gagtacggct cctattttga caggaagctt
     2041 gaagagcata tttagcgcca atgctcctcc caaaatttcg gcaagagaag ttgagacaga
     2101 ggccaacatg gctgagccta tgattggacg gcttatataa cctggaagat gcttgttggc
     2161 agcctcgctt aaacataagc cggtagcaat gccaaggtgg gcaacgttat gctgcaatat
     2221 tatcagcata atggttgcta atgtgaccat ccacaacagc gtgtacccaa agttggcccc
     2281 tgccgctatg tttgaagccc aattgccagg gtcaataaaa cctacggtta caagtaatcc
     2341 aggcccaata tatttaagaa tttcaaggcc actaaaaacc ggggtatggg ctttgttttt
     2401 taggtctttg aaaaatttga acataaacgc gtcttttttt aattattata tctgaaaccc
     2461 tggcctttgc aatttcaact ttgtcagact aatactttgt caaaagacat tgttttttat
     2521 tcttttttct cagggtataa ggttactctg atatattcag gtttaaaaac tcttttagta
     2581 atatgctgaa tatctgatga acttaatttt tcaatatcag ccttcttatt cagtatcctt
     2641 tttatgtcat ctccatacaa catcgagttt tcaatatatg atagccaaaa actattgatt
     2701 ttttcactta cttccaattc tctgagttgt gtttctttaa tcttaaccat ataactacta
     2761 tcgatgccat tttgtttaac caaatcaact atatgaaagg cttctttaac aagagaatcg
     2821 gtattggctg gagaacaagg tattttaaaa gtaattgtat agtgctgata tggaattcta
     2881 ctcatgctac aggatgcgcc aacaccgtac actccactca tttcttcacg taatacttca
     2941 taaagcctga tatctaggta gtcttccagc gaacgtaaat aataagcttg ttttgcatta
     3001 taagatatgg agtcatcaat aacaacagta accatgcttt tagggtcaga acctttgtaa
     3061 atatctttta cagtaagccc tttaggtggg cgtatgccta agtctgtata attttctgaa
     3121 cctttaataa cttttaagtt tgcaagatat attgatgcat attttttgag gctgtcaacg
     3181 ttaaagttgc cacaaatagt gtaagcgaaa tcattggcat cggcaaacct ttctttataa
     3241 atatttaaca ccttattcaa gtctattttg tcaatatctt caggcttagg cactgccgaa
     3301 cctctgggat tgttttgcga caatattttt gatacctgat ctgaataata caccattgga
     3361 tcggacatta cgtttaccag gtatgatttt atcctggtac gccacgattg ataagctaaa
     3421 gtgtctattc ttggagttgt aaagtataag tatataagct gaaacattgt ttccatatct
     3481 ttaggggtac atgaagcttt tataccttct gtatattgat tgatatatgg agacacgctc
     3541 acacttttgc ctgcaagcat ttttcgcaaa tcatcggcag aaaatttacc caatccagac
     3601 tcttcaatga tagaagcgct gaaaaatgcc gaatacatat ctttatcagg gtattttgat
     3661 tgtccgcctt tacttatggc tgaaaaaaga atttcgttgt ctttgaaatc ggtttgtttg
     3721 aaatttattt taattccatt ggcaaattcg atggtggcga ttcctgtctc tttgtttttt
     3781 gattctttag atattttctg ggctttaagc tctttgtcaa tcaacttctc atttttaaac
     3841 tcatctttat aaggtttaat atctgaagtt tcagttttat tgactattgc tattaatgag
     3901 tcttttgaag ggatagctaa tccttctttt tcgggagctg taactataat aaccctgtta
     3961 gaatcaataa gccatttaga tggaaggtta tttatttctt caaggcttat ttgaggtaaa
     4021 gtatgtttga caaggttgta ttcaaagacg atgccgggga tgctttcttt tgtcaagaag
     4081 ttgcgggcat attcttccga gaattgctct gattcagatt tatctctttc attgtagtat
     4141 ttttcaaaaa ggtttaagat ttcacttttt gccctgtcta attcagaggc agtaaatcca
     4201 aattttctaa cccttgtatt ttcttcaatt aaagcctgaa gcccttttga aataccatct
     4261 tcgctaacta aagcagacaa tatataagcg ttgttttttc ttcctaacct gctcatggtg
     4321 gcacctgcat aaatgcatgg agggttaggt ttgcggagcc gttcgtcaaa gcgcccgttg
     4381 agcatctgga agtaaagcat ccgtttaatt ccttgctgat aatctccaat tgttaattct
     4441 tgtttgaaag gtagcttgta gtataaattt acaacagttt cgttggcttc tttatctgtg
     4501 tttacagata caagcatttc tttatgaggg agaagaccga actctggcct ttcttctgtc
     4561 atttgaggag aagtcatgcc agagaagtac tttataatca agctttcaat ttcttttaca
     4621 tcaaaatcgc ctactgcaat tactgccatg ttgtttggcc tgtaccatct gttgtaaaat
     4681 cgggttatct ggcttctttt aaagctttct aatgactctt ttgtcccaat aggcaaacga
     4741 ttggcatatt ttgatttgta aaacaggatg gggaagtatt tgtccatcat cctttgctcg
     4801 gctccacggc ctaaacgcca ttcttctatg ataacacctc tttctttttc aatttcggta
     4861 ctgtcaaagg taatgttttg agcccaatca ctgaggattt caaatccttt ttcttttaag
     4921 gtatcattat ttgtagggag ctgaagtata tatactgttt catcaaagcc ggtataagca
     4981 tttatctcag ggccaaactt tatccccatg gattgaaggt agcttaccaa tttgtttttc
     5041 tcaaaatgtt tggtaccgtt aaaggccatg tgttctgtaa agtgggcaag cccaatttgg
     5101 tctgagtctt ccaatataga cccggcattt actacaagcc ttatatccat gcgtttttcc
     5161 ggcctgacgt ttttcttaat ataatatttt aaaccgttgg gtaatgtttt gataacaatt
     5221 gagctgtctg ttgggattaa tgcgttgcta tcaaaatttt gaccagaaat aacagcgaaa
     5281 gcaatttgag aaataaatgc aaaaagtaat attttgtttt tcattgtgaa atgaagttta
     5341 aggttaggta caataattta ttaaaacatg cttagaacag aatgttgtct aaagaatgct
     5401 taggtttatt tttttagtgt cttacttttg ggctttgagt tcagccggaa ctttaattgg
     5461 gtcaattttc aaacgggcat ccgggcaatt gaccagctca aattcaaccc gtcggttgaa
     5521 agccctgcca accgggttat cgctgccatc agtattagca ttaatggcaa ttggttcatt
     5581 taaacttcta ccttcggtaa taatctgttt ttcgtcaact ttcaatgcta ataaaacgtt
     5641 ttttacaacc ccggctcttt tttcagaaag cttttggtta tattcttcag agcctaatgc
     5701 atcggtatgt ccaattaaac ggatttgcat ttcagggtat tttttcaaca tattggctat
     5761 agtcacaatt tcttttttat cgtcatcatt aatttttaca tcattaaacg aaaacaaaat
     5821 attcctgata agaataggtt tgttaggttc ttcaatagag ttgataaatg agctataggc
     5881 aattttatcc tggatgattt ctgtagttag tgtatatgta tcggatggat aattttcggg
     5941 aattacaaac ctgtttgaaa tgactttgct tgtgtctttt atgaatagca ctctataagt
     6001 gtccgcagct ttagaaaact caaatgaacc gtctgcattg gctttgatgg tatcgctaat
     6061 aactttgccc gaatcattgg tcaaaagtat attgtaattt tcagcttttg aaatttgtaa
     6121 taaatagttt acattgcctt ttattgtaaa tggaaagggc cggccatgat taaaaaattc
     6181 aatcttacaa atgtcaaagt tccccatgcc tcctgttttt aaatatcttg attgataggc
     6241 tgtataactg ttggcaacag ggatgaaaaa taaatcatca tcggtagtat ttacagggta
     6301 tcctatgttt ataggagcag accaattgcc attactatct tttacagaat aaaaaatatc
     6361 aaaacctccc attgattcgt gaccttgtga ggcaaaatat aatgtatccc ctgaataaga
     6421 aaggtatggg caatcttcgt ttaatttggt attgatagta tttccaatat ttacagcagg
     6481 cccccattcg cctgttgaag tttttatgct gtaaaaaata tctaatcctc caacagattc
     6541 tggtctgtta cttgaaaaga aaatagtatc accccctgga gataaacatg cgttagattc
     6601 ccaataacgg ctgtttattg ctttgctaag cggctgagag ggttgccatt ttttcttttc
     6661 aaattttgcc atgtaaatat cgctgttttc tccatctata tagtttagca acaatgtatt
     6721 gccatctggt gaaaggtaaa gagggaactg gttgccatca ctcatgatat tcccggtaat
     6781 gttaagaggg agcccccatt tgtttgactt gtttttttca gaaaacatca aagcataata
     6841 aaacttctgc ttggtaatgt aaaccattct tttttcattg gccgaaatag ccgggtttat
     6901 attgtcttct ttcgtgttta taggttttcc caaagagctt atttttatcc ggacaatgga
     6961 atttgttgct gcttttgccc gttgacaggc ttcaatctga tttctggccc attctttttc
     7021 catttgtagt tccttggggt tggaggtcaa tgcgagaaat ttattgtatg cttcaatggc
     7081 tttttcatat tgcatattga tacgatacgc attgcccaaa aataaaagta cgtcaatagg
     7141 tgccttagtt tcttttatag agccttcttt gtaattattg gtaatgttta aggctgcata
     7201 ttcaaggaaa gggattgact tctctttttg tccggcaatg ttcaggtagc agacacctat
     7261 tttgtaattg atattggggt tatttttaaa gttttttttg tagagtttta agtatgaaga
     7321 tattgcctca gtataatctt cctgtacaaa aaaatactca gcatcgtcaa atgtggcttt
     7381 gtcataagct gtttgtccta aagcgtaagt tcctgttaga gggaggaata ttactaatag
     7441 cgctaatatt ttcattttca tttatttaac ctcttaatta ttaaccctaa gcaaagatat
     7501 aaatctttgc atgataatta aattttattt tgcatccaaa ttagggatat aaatagaggc
     7561 atattaaaaa caaaacccgg cttgaaatgt ttcgtgccgg gttgtaagct tgttatgggc
     7621 gtgtattagg ctgcaaaatc ttttaatttt tcggtcagtt tttttcttga aaagaaaatg
     7681 cggcttttga cggttccgat gttaaggtta agttcatctg caatttcttt gtatttgtat
     7741 ccatcatggt gcatttgaaa ggggatacgg aattcgtctt caagctcatt gatggtcttt
     7801 gtcagctctt ctgttgaata tgacgaatct gggtcgtcat ttcccgttac actcatgtgg
     7861 tttaaaaagt atccttcttt ggtattgtcc ctgtgcgtgt tctggcgtat gtttcttcta
     7921 tagttattga taaatgtgtt tttcatgatg gtatatgtcc acgccttaaa attatcgttt
     7981 tgcacaaatt tatctttata ggtcaatgct tttaaaaagg tttcctgcaa caggtcttta
     8041 gcatcttctc tgtttaatgt caaactataa gcaaaacgtt caaggcttcc ttctaagcct
     8101 accagtttat gttgaaattc tatagctgtc ataatatttt gttttaaatt gttgaatcaa
     8161 agttatgaca aattaaacag ataaaaaaat cttaatatat taaaatgcta aaatatgcta
     8221 ttaattttga ataaacattt ttttgtaaaa aacataatat caattaaata taccgaaaaa
     8281 aaagcagctt ttcaaaatta tttctaaata acatcgttaa acaattttct ataatttggt
     8341 attgaagagt ttaacaaaaa ttatttagaa ttagtttaaa taagctcatt ttcaataagt
     8401 tgcttttttg cttcattgat tatggttaat gtggtaaact ggagtacttt taaaggcaaa
     8461 agcaacaaca atttattgag atgcatttgt ttttgaaata cctgttcatt ctttttgcat
     8521 tgctttggta cggtatgttt aatgtttatg cacagcacga aggaagcggg attccattta
     8581 gccgaaatac tattgctgaa gatgcttttc cattgataga acttatgcct attgattctt
     8641 ctgagatagt tgcagctaaa tctcagaaaa tagccgtaaa tgctattgtt agagaaattg
     8701 acattcgaac agcttctgaa ggtgcgtggt ataaaattaa ggatagaaac gtttgggttg
     8761 caaggctcaa atctgcttat gcccagtcgc ttagttttat gattgaaaat tttttcctta
     8821 aaaatggtga gaagctcttt atttataacg cttacgaagt gcttggggct tataccaaag
     8881 catcaaaccg cgcaaataga aaatttgcaa cgcgccagat aaagggtgat aatgttataa
     8941 ttgagttttc tacaccatat ccggttgcag aaagtggaac tttcacgttt acttcggttt
     9001 ttcatagttt ttcggttgac gaatatactt cgtgcaatat caatgccagt tgcatcgaag
     9061 gagataactg gaaaaaggaa cgtagggcca tttgcaaaat taatgttggc gccagaaact
     9121 gtaccggagt attgataaat aatactctta aagatgaaac cccttatgta cttacagcta
     9181 atcattgcat ctgggataat accgaagccg aaaacagtgt gtttacattt aattacgaaa
     9241 cttctgcatg taatagccag atagcttatg ctggccatgt gttgtcaggg gcaactgtca
     9301 tgtgtggcag gtacgaaaat gattatacat tgctcaaact tatggaccat ccaccggttt
     9361 cgtttatgcc atattatgcc ggctggtcgc tcaggtctga tgaaaacctt gacactgtta
     9421 cagttttgca ccatgcgggg gggacacctt taaagatttc acaaagtgta tcgcggcctt
     9481 tagacagtac tttcaaagaa gatgatgagc cagcttatgc caatggcgct ttttggctgg
     9541 ttcaaaaata taattttggt attaccgaaa caggttcgtc cggggccccg ctgtttgata
     9601 aggaacacct cattgttggt actttatctg gtggagatgc ttcaaactta caatgcaaaa
     9661 atgatttgaa tgattactat cagatgttca acaaaagcta tacagtacag cccttgacag
     9721 gcttgcccat gatcaactac ctgaacccca acaatgcacc tgttgcgtca ttagccgggt
     9781 ttgatccttt ggaaaaaaat gtttacgggt gcgatacttt ctcgaatata gcagatacgg
     9841 caggacgcgt gttacatcta tatccttatg ggaatggcta tttagccggg cacaatgaag
     9901 atagtattag ccagtttgga gaagtgtttg aaaacgctga ttcaattgtc ctttacgggg
     9961 cttggtttca tgttgcaaaa tgtgggacaa acggcaatat tgtggttagg gccttcaatg
    10021 gctcagactt ctcatccagc gcggtttttg aaaaagtgat ttctcaaagc cagatggtaa
    10081 aagacaaata tcactacatt gaattttatc cgggaataaa tctgacgggt aattacacta
    10141 ttacctttca ggtcgaatat cagcattctg atacttttgc tgtttatatg tctgctttga
    10201 ataatgaagc taaagtttct tctgctataa taaataataa cggatggcaa aatatgactg
    10261 atttcttgcc tggtcatgag tccgtttctt ttgatattag cgctatgtat tgcaaagaaa
    10321 aaaataaaac agcaataaat aaggccgaga tatcgcagtt aaaggtgttt cccaatccgg
    10381 caaaggattg ggtagttgta aatatcgaag ggttgaccaa tccagaggtg caattgttca
    10441 atatttatgg gcaggttgta aatatttcgt tgtccataaa caatagtttt attaaattta
    10501 atgtttcaaa ccttaaatct ggtatatatt tgataaaaat tagttgtaaa aacaggctga
    10561 taattggaaa atttttgaaa gtttaattta tcaaacattt ttattttctt tgattctttt
    10621 aaaactaaaa tatgataaaa tacccatgat tcggcaaaac acaactaagc ccaagagctc
    10681 tcaagagcaa agcaaattaa ttgcaacatt atttatttgc gggcaccatg ttatcattcc
    10741 attaaaaatt aaaaaattaa tatgatgaaa ctgaaaattt ggctttgttt attacctgtt
    10801 attttttaca gtattaattt tttgggacag ggaaaaaaga aagtttccat aactacagcc
    10861 attgaggtac aaaatgaaga cggcgagggg ctgtctggcg tatcggccaa actttttaac
    10921 aaaggtcagg aagaaaaatc tgttgttacc accaatagcg gaattgttac atttacatta
    10981 gagcccaaca gtgattattt gatagaactt tctaaagatg gttttgtctc taaaattatt
    11041 agtgtaaata caaaagtacc ctcatatgaa gaccaacaat ttgaagtggc ttttcctgtg
    11101 ctcttgttcc agccttgcga agggcttgat tactcaattt tgcaaaagcc tgtagtgaag
    11161 ctggttcata acgaaaaaaa acgggatttt ttgcccgaaa aagtttatga cgaagtgatg
    11221 caggagaaac ttagcaaatt atacgaagat aaccagaatt gtttggatga aaagtacaat
    11281 gccattgttc gtgaaggcga taggcttttt aaaagtaaaa aatatgaaga ggctgtctct
    11341 ccttaccaaa aagcgctggg gataaggcct aatgataaat atgtcaaaag ccaattagat
    11401 gcaattgaca agctattggc cgaatcaaaa gataaagaag caacctacaa aaagtatatt
    11461 gaacaggctg acaagcaaat ggctgataaa aattacccct tagctaaaga attttataat
    11521 aaatcacttg gggttaagcc tggttctgca tatcctacgt ctcaaatagc cgaaatagat
    11581 aaaattttag cccagcaaaa acaggatgaa accaataaac aacagttgca acaacaggta
    11641 acaagcttag tggcccaggg cgatgggtct gatgattact gtggagctgt tcttaaggct
    11701 tataaagatg ctttagcctt aaaacccaat gaccctgcta ttctggctaa gatatcggct
    11761 gctgaaaaaa aatgtaatga catcaaagcg gcaatagcta atgataaaaa gaagcaggaa
    11821 cagtacaaag cttctattgc caaagcagat agtttataca aagccaataa atatgatgct
    11881 gctattgctg agtatcaaaa cgcctcgaaa attatccctg ctgaaaaata tcccaaagat
    11941 caggttgatt tgattaatga tattgtcaaa aagcaatcga aagacagaga tgctcaattt
    12001 aaagcccttg tggcacaagg cgataagtca gaggattatt gtggtaccgt ccaaaactcg
    12061 tatcgggaag cattaaaact taagccaaat gatccagaat tgcttaccaa aatctctatt
    12121 gccgaaaaaa aatgtaatga tgcaaatgct cagcaagcag atttaaaaaa aagacaggca
    12181 ggttacaaag cttctgttgc taaagctgac agcctttata aagccaataa atacgatgct
    12241 gctattgccg agtatcaaaa tgccatgaaa tttgtccctt cagaaaaata ccctcaggat
    12301 cagattgact taataaatga aatggtcaga aaacaaatga aggatgccga atcgcagtat
    12361 aaatctttga ttaaggaggc agacaaagca tttgacaatg aaaggtttga ttttgctaag
    12421 gatagttatt taaaagcatc taaaattaaa cctaatgaag cttatccgaa tgataaaata
    12481 aaagaaatcg ataatattat tacagcccaa aaggttgctg aggctaaacg caaggaaatt
    12541 gaagagaaat acaaacaaaa gattgctgag gccgatgctg caatgaaaaa caataattat
    12601 atgatggcca aaaacctcta taatgaagca ttgacaataa agttagaaaa atatccatca
    12661 gatagaattg aagagataga tcaactgatg aaagcccagg cagataaggc tgaaaaagaa
    12721 tatcagtcta aagtgaaatc ggctgataaa aattttgttg attcaaagct gaaagaagcc
    12781 ttggccgatt atcaggctgc attagcaatg aagcccaatg aaacctatcc tcaacaacgt
    12841 atagacgcta taaacaaaat gttagaagaa aaagctaaga aagaagctga ggaaaaagcc
    12901 aagaaagatg cttatgcaag tgctatagcc agagctgata agcttttagc tgaaaataat
    12961 tttgatcagg ctgcagctgc ttataaagaa gcttcgtcat atcagccttc tgaacaatat
    13021 ccttatgcca agatagatga gattgcaaaa ctaaaaaaac aaaaagaaac agaagcaagc
    13081 tatgctaaag caattattac tggtgacgat tatttcaaca aaaaacagct acagcaggca
    13141 aaatcttttt ataatcaggc attaggcttt aaacctggcg ataaatctgc aacagataag
    13201 attgcaaatg ttgacaggct aattaatgaa gatattaaaa ggcttgctga cgaaaaagca
    13261 cgtaaagatg cctatgataa agctatcatc gatgctgata aaagtcttgc tttaaaagat
    13321 tatgatatgg ccaaagcttc ttatcaaaca gcattatcta ttgatgcaaa ccaagcttat
    13381 ccgagacaaa aaatagctga agttgataag ttgattaaag aaaaagaaat taacgacaat
    13441 tatcaatcgg cacttgatga agctcaaaaa ctttttgatg ccaaaagttt tgatatggca
    13501 aaaactaaat atagagaagc taaaaaaata aagccttctg aaacttttcc tgatactaaa
    13561 gtgaaagaaa tagaagcagc tcaggctcaa atggaaaaag accgtttaca aaaagagaaa
    13621 aaagaaaagg attacaaagc ctctgttcag ctggccgaaa gcctttttga aaatgccaga
    13681 tatgacgagg cccagaaaga atatgaaaag gctttgctta ttttgcctga tgaagtatat
    13741 cccaaacaaa aaatagctaa aataaaagaa ataaagatgc ttcttgaaaa agaagctaaa
    13801 gccagcaaaa ctgcagcagc tccggcacct aaaaaggctg agcctgttaa aggaaaattg
    13861 gataaattgg tgtttgcaaa tgaatcagaa agggctcagt ttcttagcga tttgcttact
    13921 tcttatccgc ctgggattac ccatgaagta tataaagaca agataaagac tgttaacagg
    13981 tacattatta tcagggataa tcaggcaaat gacttcagat ctatccgtct gagctatggt
    14041 atggagtatt ataaaaatga taaaccaata actgagcagt atttcaattc tcaggttaag
    14101 gtaagggcag gagaatatta caataaggtt gatttataaa atatctatga ttgtttaaat
    14161 ctcacaatac gaataatatt caaataagaa ggagaataac ttgtattgtg acaaaaaaat
    14221 tgatttgttg aagaagcaat agctttgagg tattgccata aaaagaatgt aatggctgag
    14281 atgttgaaaa ttcatattat gaagtaaaaa aaaaacacat tattctttga caaagaagaa
    14341 taatgtgttt tttttagaga aaagcagatt aatctactat ttctatcatt ttaaatggca
    14401 cac
//
LOCUS       DAOSPF010000074        14346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4251_length_14346_cov_7.992023, whole genome shotgun sequence.
ACCESSION   DAOSPF010000074 DAOSPF010000000
VERSION     DAOSPF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_14346_cov_7.992023"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1128
                     /locus_tag="PK252_12200"
     CDS             199..1128
                     /locus_tag="PK252_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOT15514.1"
                     /translation="MRNLLIIFSIFLVAGCSTQQKVVQTQPQIQEIKKDERSLKRVVA
                     ISRFSNETQYAKGIFYDKANDPMGKQAVDILSAKLASTGKFILLERQDLDKIVAESKI
                     AGTDSIQHIRADYIILGSVTQFGRKTVGDQNVFSRSKTQTVEATVSIRLVDVYSGQVI
                     YSEEGKGEAETTAKSVMGIGEHADYDATLSDKAISAAISKLVENIINNCMDRPWKSYF
                     LAYDDNGIIIAGGKSQGLKVGDIFIIEEKGKKVKNPQTGMTIKLPGKVAGKIKIDFVG
                     GDNPENEFSMVSFIEGSIDKQNLKNYYVKEDKK"
     gene            1125..1457
                     /locus_tag="PK252_12205"
     CDS             1125..1457
                     /locus_tag="PK252_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15515.1"
                     /translation="MKKIIFLLAAIIGLMTSCTGVKTSASGVDNQAFINIVGKPGNYS
                     GGVDVNVDDKTNFKAQVNKEYTDRPKGKVYAISTGTHVVTVSYNGQVLYKQQIFVSSQ
                     ETKKIVLP"
     gene            1523..1876
                     /locus_tag="PK252_12210"
     CDS             1523..1876
                     /locus_tag="PK252_12210"
                     /inference="COORDINATES: protein motif:HMM:NF027394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HOT15516.1"
                     /translation="MNKLIIVAVGALLMASCTVENQLYSWYAYDEASYNFLKNNDEKS
                     RQELIENYQQIIEKQRGTRKVPPPGIYADYGFLLLQANKTVEGKALLEKEIALYPESK
                     VFIERILKMIEDEKK"
     gene            1863..2546
                     /locus_tag="PK252_12215"
     CDS             1863..2546
                     /locus_tag="PK252_12215"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOT15517.1"
                     /translation="MKRNSIYLIIFACAMLVTSCTTTEPLKKSEAYKNMYSEKPLTVL
                     LMAPINRSTNVDAKQYFHSTLALPLANNGFYVIPPFLSMEILQKESAYDSEMFLNAPL
                     TKFGEVFGADAALFTIIHKWNKSYSHVIVEVEYILKSTKTNEILYSRHGVVDYNTAVT
                     TKAGGIAGLIADLALTALNTATTKYVDVARKCNGYTFTDIPAGKYSPSYNLDGENNAG
                     DKTFRVNLQ"
     gene            complement(2608..3027)
                     /locus_tag="PK252_12220"
     CDS             complement(2608..3027)
                     /locus_tag="PK252_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOT15518.1"
                     /translation="MNALVVYQTKTGTTKKMANEIAAQLAQNNARVKVASIDEVSHDE
                     FLAAEQIYIGCPTEGLFIFGQKPTKDWKKFVNKLPVMANKRAVMFTTYKVATGSMFKK
                     MKSELRYLGLNVDNRAFRSKDGLLNEVQKKVLIETMN"
     gene            complement(3146..4066)
                     /locus_tag="PK252_12225"
     CDS             complement(3146..4066)
                     /locus_tag="PK252_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOT15519.1"
                     /translation="MFCRIVYILFISLFVFTAKAQELKCNISINSQKVQGSNKTIYTT
                     MQSAIYEFMNNRSWTNHVFKVNEKIECNILITINEQLGADEFIGTIQIQSRRPVFNSS
                     YNTVMFNYNDQNFNFKYVEYQAIEFNENTHQLNLASVLAYYAYIIIGLDYDSFSLLGG
                     TEFFNKAEKIVNNAQNSSDKGWKPFEAKSNRNRYWLVKNILDKRYEPAREFIYRYHRL
                     GLDLMNSKPVEGRNEATEALLLLQKVYRQKPDPFLHFMQVIYDAKSDEFVNFFSEGMP
                     DEKNRVYQILNEIDNANTTKYKKITSTESN"
     gene            complement(4143..5351)
                     /gene="coaBC"
                     /locus_tag="PK252_12230"
     CDS             complement(4143..5351)
                     /gene="coaBC"
                     /locus_tag="PK252_12230"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOT15520.1"
                     /translation="MLKGKNILLGITGSIAAYKAANLVRLLVKEGADVRVLMTPLAKE
                     FITPLTLATLSKHSILVDFFDPTNGQWNSHVDIGMWADAYVIAPATANTIGKMANGIA
                     DNLLLTTYLSARCQVFVAPAMDLDMFAHVTTQNNLKTLANQGVKIIEPSTGELASGLE
                     GKGRMEEPEKIVAAISAFFETAKRLDGKKILVTAGPTFENIDPVRFIGNYSSGKMGYA
                     LAEECANEGAEVFLISGPVAVKTVHKNIKVISVVSADEMLEQCLKYFSVCDAAIMAAA
                     VADFKPKEKEKSKIKRQEREGMVIELVPNPDIAANLGSLKNKNQVLVGFALETDNMLE
                     NAKGKLSKKNLDFIVLNPANVENAGFKSDNNIITIIDQTGDVKAYENKPKHLVAKDIC
                     DCLYLHLQKK"
     gene            complement(5368..5697)
                     /locus_tag="PK252_12235"
     CDS             complement(5368..5697)
                     /locus_tag="PK252_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT15521.1"
                     /translation="MASDYKKSKAPSSTITRDLDKLESVTGNIYETVVVLYKRANQIS
                     VEIKDELNRKLQEYGNSNDNLEEIFENREQIEVSKFYERLPKPTLIALQEYEDNEIFF
                     KKTGKKE"
     gene            complement(5711..6511)
                     /gene="bamD"
                     /locus_tag="PK252_12240"
     CDS             complement(5711..6511)
                     /gene="bamD"
                     /locus_tag="PK252_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOT15522.1"
                     /translation="MQKNSLLTIFLVAVSTLIIVSCSEYEKVLKSNDYDFKYRKAVQY
                     YEKKDYARAITIFEQIVSYFRASTKGDSLMYLFAQSYYGDEDYTMAGFYFKELSDNYA
                     RSPFVEEADYMVGYCYYLVSPRANLDQENTNLAVAAFQKFIYKHPSSKYTPECKRLIK
                     ELQDKLAEKAYLNAKLYYDLKYYKAAIIALNNCLTQYPESSHREKLMFMLLDAKYELA
                     LNSITEKQKERFQNALDEYYSYVAEYPKGQYDKQVTKIFNKAKEVLGL"
     gene            complement(6748..6831)
                     /locus_tag="PK252_12245"
     tRNA            complement(6748..6831)
                     /locus_tag="PK252_12245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6795..6797),aa:Leu,seq:caa)
     gene            complement(6901..8289)
                     /locus_tag="PK252_12250"
     CDS             complement(6901..8289)
                     /locus_tag="PK252_12250"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT15523.1"
                     /translation="MLTVEKIGGTSMSQFGDVVKNIIIGSRKEGDYYNRIMVVSAYNN
                     VTNWLLEHKKTKEPGIYARFAEGRDYNEALADLLKKLIEINHSFENIGLDLKAADHFI
                     STRIVQTRNYLNSLADVLATGYVSRQSILLAAREILASIGEAHSGFNSVNILNNRGVN
                     AKFIDLCGFNDPEYLTIDERIHKAFQGIDFSKVLCIATGYTKGTEGIMREFDRGYSEV
                     TFSKIAVEVKADEAIIHKEFHLSSADPKIVGVEKSIPVGFTNYNVADQLADVGMEAIH
                     PKASKPIEIAGINIRIKNTFEPEHPGTLISKDYISKESKIEIVTGSDKVTAIEVHDPF
                     MVGEVGFDYAIMEFFKKYNISYILKSSNANSITTVIWQDEVYPEFIADLQKSFHKITV
                     KNVALVCTLGTNIAKPGILAKATTALSQNNINIDCISQSLSQVSIQFVIEREHYNKAI
                     AALNDALCVKNN"
     gene            complement(8677..9510)
                     /locus_tag="PK252_12255"
     CDS             complement(8677..9510)
                     /locus_tag="PK252_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15524.1"
                     /translation="MYFTSNLKFLRKRKRRTQEDVSAALGIKRPTYNGYENGVAEPSI
                     DTLIACSKYFNISIDSLVKVDLSKLSEFQLGELERGNDAYLSGTKLRVLATTVNKENE
                     DNIELVSEKAKAGYATGFADPEYIQELPVFQLPFLSKQKKYRTFQITGDSMLPIPDKA
                     WITGVFIQDWRSIISGHAYIVLTLDEGVVFKIAENLISEENKLRLYSLNPIYQPYDLH
                     VSQIREIWQFVNYISSELPGPMEPKDQLAQTIDAIRDDVKYIKDVLGRKDESQRKTRC
                     K"
     gene            complement(9653..10108)
                     /locus_tag="PK252_12260"
     CDS             complement(9653..10108)
                     /locus_tag="PK252_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15525.1"
                     /translation="MRLNFFSLNELNFVEFNITGDNDTFMPLQEESAYLGSEVFNLFL
                     SCFENSNELYEYFEPTRFNSRKIVVLRNELISFLDKLNSIDSLMTLETFINSLFLGKE
                     FFKKLESDSVHWKDNWASYLEQLKDVNKKMIAIVERCIDEGRILWVVGY"
     gene            10224..11660
                     /locus_tag="PK252_12265"
     CDS             10224..11660
                     /locus_tag="PK252_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT15526.1"
                     /translation="MTDHITDIKQLYMQNFGKEPENIVALPPSGSNRKYYRISNGNDS
                     CIGAFNEDFKENKAFINFTKIFLHHELPVPKIIDENLDKHIYLLEDLGNNTLLSLIEG
                     NLDKGLQPENIALYKKVLDYLPIFQVVAGKDIDYSVCYPRNAFDKQSMMWDLNYFKYY
                     FLKMAQIPFDEQLLEIDFECFCDYLLQTDCNHFLYRDFQSRNVMIKDGEPYFIDYQGG
                     RKGALQYDIASMLFEAKTHLKPEVRQELYEYYISVLKKYHSFDEEKFTSHYYAYIYIR
                     LMQAMGAYGFRGLYERKELFLQSIPKALEHLRWLRENVKLPLNMPQLERVWDFLVENE
                     HIRNLAKTPNYLTVHISSFSYKEGIPSDSSVHGGGFVFDCRALENPGRYDEYKSLTGK
                     DAEVIKFLESKSDVQTFFAHVSSIISQSVTTYQKREFDYLVVNFGCTGGQHRSVYMAE
                     KLAQYLKKQFSHIKIDLQHCQLDKIKAQ"
     gene            11657..12376
                     /locus_tag="PK252_12270"
     CDS             11657..12376
                     /locus_tag="PK252_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT15527.1"
                     /translation="MKAMIFAAGLGTRLRPLTDNKPKALVEINGKTLLEITIQKLKAA
                     GFNSIVVNVHHFSGLVIDFLKKNDNFGLDIEISDETDLLLDTGGGLKKAAPLLGTQEP
                     VLIHNVDIVSNIDIGELYTQHCQSSALATLATMERDSSRQLLVNSKGILCGWQNKSTG
                     EKKISQPSETDLTPISYCGIAVLNPQFLTLIEENGVFSIIDVYLRLAKNFDIQTANQE
                     NINWSDVGTVESLKRLRRNVN"
     gene            12382..13095
                     /locus_tag="PK252_12275"
     CDS             12382..13095
                     /locus_tag="PK252_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT15528.1"
                     /translation="MKLSIIIPLYNEERYILSTLKKVEEVIIPKSINDVEIIVIDDCS
                     KDNSWQIINEYVKNKENVKLFRHDVNKGKGAAMRTGIANCTGDLVVFQDADLELSPED
                     IPVMIDTMIKLDVDFVNGSRYLSGVPRPLSSFWRYIGNKIFTLLTAMIINVKISDMAC
                     GYKLVKKDLLDKLNLKENRFGIEAEIMIKALRFKKERIAEVPVQYLPRTQQEGKKIRN
                     SDAFKIFYKILKYGLFKAS"
     gene            13073..>14346
                     /locus_tag="PK252_12280"
     CDS             13073..>14346
                     /locus_tag="PK252_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15529.1"
                     /translation="MAFLKLRNKVKTWSISLILVFLSIFLISNVSKNISLYNKQEAVI
                     EWDAISLYAYLPAFFIYNDIKLDFVQPNGKTGDCSYTIWYSENNKGQRIIKTSMGVSY
                     MYLPFFLGAHWYSSITNNPTCGYSPIYKIGIIISCLFYLFLGLFFLRKILLKYYSELV
                     TSIVLIILLAGTSLSYYSIFLPAYTHIFSFALVTMFLYFSIKWNEAPRFTYSIALGLL
                     IGVITLIRPTNFLIVLVWILYNIYNLNSLKLRIKFFFTHYSKILLIAFLAFLTWIPQL
                     IYWKYVTGDFFYYSYGENERFFFNHPHIIEGLFGFRKGFFIYTPIMIFAFIGLYYYFK
                     QNKALAIPIIIYSLLTIYVTLSWWCWWYGGCLGMRPFIDSYAIWCLPLAALINQLNSQ
                     KIGWIIGIYLSMSIFIWFNFMYMKKYRTGGVH"
BASE COUNT         4655 a         2733 c         2454 g         4504 t
ORIGIN      
        1 aaataaacat aattcttggg ggaatacaat aaaacttctg tcttaaagat aaaatcaaat
       61 gtcattcacc ctccatttta tacaacattt aaggcctcct ccgttttctt taatgcacct
      121 tctaaatatt ttacctgtag ttttttttga ataatatttt aaaaaatcta aatttaccta
      181 tcataaaaaa caacattcat gagaaattta ttgataatct ttagcatttt tctcgttgca
      241 ggctgttcaa cccagcaaaa agtggttcag acccagcctc aaatacaaga aataaaaaaa
      301 gatgaacgct cactaaaaag agtagttgct atttcaagat tttcaaacga aacgcaatat
      361 gccaaaggca tcttttacga caaagccaac gaccccatgg gcaaacaggc tgtcgatatc
      421 ctttctgcca aactggcttc aacaggcaag ttcatcttgc tcgaaagaca agacctggac
      481 aagattgtag ccgaatcaaa aattgcaggc actgacagta ttcaacacat acgtgccgac
      541 tacatcatcc ttggctcggt aacccaattt ggaagaaaaa ctgttggcga ccaaaacgtt
      601 ttctcaagaa gcaaaaccca aaccgttgaa gctacggtta gcatccgttt agttgatgtt
      661 tattcggggc aggttatcta ttcggaagaa ggtaaaggcg aggccgaaac aaccgctaaa
      721 tctgtcatgg gcattggcga gcacgccgat tacgatgcca cactaagcga caaggctatc
      781 tctgctgcca tctcgaaact cgttgaaaac attatcaaca actgcatgga ccgcccctgg
      841 aaatcatatt ttctcgctta tgacgataat ggcatcatta tagccggtgg caaaagccaa
      901 ggtttaaagg taggcgatat tttcattata gaagaaaaag gcaaaaaagt taaaaacccc
      961 caaaccggca tgacaataaa acttccgggc aaagtagccg gaaaaataaa aattgacttt
     1021 gtcggtggcg acaaccctga aaatgaattt tcaatggttt cttttatcga aggaagtatt
     1081 gacaaacaaa acttaaaaaa ctattacgtt aaggaggaca aaaaatgaaa aaaataatat
     1141 ttctcttagc tgctatcatt ggtttaatga ccagctgcac tggcgtaaaa acgtctgctt
     1201 caggagttga caaccaggcc ttcattaaca ttgtaggaaa accaggaaat tattcaggcg
     1261 gggtggatgt taacgtggac gataaaacca actttaaagc ccaagtgaat aaagaataca
     1321 ctgacaggcc gaaaggaaag gtttatgcca tcagtaccgg cacccacgtg gttacagtat
     1381 catacaatgg ccaggtttta tacaaacaac aaatctttgt gtcgtcacaa gaaaccaaaa
     1441 aaatagtttt accataacat tacacaaaag cctcattact aaacccaatg aggctttttt
     1501 tacaaaccta aatcacactt tcatgaacaa actaattatt gtagctgtgg gggccttgct
     1561 aatggcttcg tgcacagtag aaaaccagct ttactcctgg tatgcgtatg acgaagcatc
     1621 gtacaatttt cttaaaaaca atgatgaaaa atcaaggcag gagttaatag aaaactacca
     1681 acaaatcatt gaaaaacaaa gaggaacaag aaaagtgccc cctcctggca tatatgcaga
     1741 ctatgggttc cttttgcttc aagccaacaa aaccgttgaa gggaaagctt tgcttgaaaa
     1801 agaaatagcc ctttaccccg aatcaaaagt ttttattgaa cgaatcctta aaatgataga
     1861 agatgaaaag aaatagcata tacctcataa tatttgcttg tgctatgctt gttacaagct
     1921 gtacaacaac agaacccctt aaaaagtccg aagcatataa aaatatgtat tcagaaaaac
     1981 ctttgacagt gctattgatg gcgcctatca acaggagtac aaatgttgac gcaaagcaat
     2041 attttcattc aacactggcc ttgcctcttg ccaacaatgg attttacgtt atcccaccat
     2101 tcctgtctat ggaaatattg caaaaagaaa gcgcttacga ctctgaaatg tttttaaatg
     2161 cacctcttac caaatttggc gaagtattcg gagccgatgc tgcccttttt actatcatcc
     2221 acaaatggaa caaatcttat tcgcacgtga ttgttgaagt tgagtacatt ttaaaatcaa
     2281 ctaaaacaaa cgaaatattg tattccagac atggagtagt cgactacaac acagcagtta
     2341 caacaaaggc aggtggtatt gccggactta tagccgacct cgctttgaca gcattgaaca
     2401 ctgccacaac aaaatatgtt gatgtggcca gaaaatgtaa cggttatacc tttaccgaca
     2461 tacctgctgg taaatatagc ccttcttata accttgatgg agaaaacaac gcaggggata
     2521 aaacgttcag agttaatttg caataacaaa aaaagccctg aaataaacaa cttcagggct
     2581 tttacatttt ataaacaata ccacgaacta attcattgtt tctatcaaca ctttcttttg
     2641 aacttcattc aacaaaccat ctttcgacct gaaagcacga ttatctacat taagccccaa
     2701 ataacggagt tctgatttca tttttttaaa catactacca gtagccacct tatatgtggt
     2761 aaacattaca gctcttttgt tggccatgac aggtagttta ttaacaaact ttttccaatc
     2821 tttggtaggt ttctggccaa aaataaacaa gccttcagtc ggacatccta tatatatctg
     2881 ctctgcagct aaaaattcgt catgcgacac ctcgtcaata gaagcaactt taacacgggc
     2941 attgttttgt gcaagctggg cagctatttc attggccatc ttcttggtgg tacccgtttt
     3001 ggtctggtaa actactaatg cattcataac ttttctattt aattatgaag caaaattacc
     3061 agcatcatta ctgatataaa acctaaaaaa ggtgaacagg caaaaaacaa ggatgaatta
     3121 gcttttttgc tacattacca ggaagctaat tgctttctgt cgaagtaatt tttttgtact
     3181 tggtagtatt ggcattgtct atctcgttca gtatctgata aacacgattt ttttcatcgg
     3241 gcataccctc actaaaaaaa ttgacaaatt cgtcggattt agcatcatag attacttgca
     3301 taaaatgcaa aaaaggatct ggcttttgtc tatagacttt ttgcaataat aaaagtgcct
     3361 ctgttgcttc attgcggcct tctacaggtt tgctattcat caaatcgagc cccaacctgt
     3421 gatagcggta aataaactcc cgagccggct cataacgttt gtcaaggata tttttcacca
     3481 accaatacct gttgcggtta ctttttgctt caaaaggctt ccagccttta tcactcgaat
     3541 tttgggcatt gttaactatc ttttcagctt tattgaaaaa ctccgtacca cccaagagcg
     3601 aaaaggaatc ataatcaagc ccgataataa tataagcata ataagccaag acggaagcaa
     3661 ggtttaattg atgtgtattc tcattaaatt caatagcttg atactcaaca tacttaaaat
     3721 taaaattttg atcattataa ttaaacatca ccgtattgta agacgaatta aaaaccggac
     3781 gacgcgattg tatttggata gtaccaataa attcatctgc accaagttgt tcattaatgg
     3841 taatcaaaat attgcactca attttttcgt tcactttaaa aacatgattg gtccatgagc
     3901 ggttgttcat aaactcgtat atggcgcttt gcatagtggt atatatggtt ttgttagaac
     3961 cctggacctt ttgcgagttt atactgatgt tacatttaag ttcctgtgct tttgcagtaa
     4021 aaacaaaaag tgaaataaac aatatataaa cgattctaca aaacataatt tccgaatatt
     4081 ttacaataat aacctttaat tgaggtataa attgttttgc acagcaaatt tcttttaacc
     4141 agttattttt tttgaaggtg cagatataaa caatcgcata tatcctttgc aacgagatgt
     4201 ttgggcttat tctcgtaagc tttaacatct ccggtttggt caataatggt tataatatta
     4261 ttatctgatt taaaacctgc attttcaaca ttggcaggat taaggacaat aaagtcaagg
     4321 ttctttttgc tcaacttgcc tttagcattt tcaagcatat tgtctgtttc taaagcaaaa
     4381 ccaaccaata cctggttctt atttttcaaa ctacctaaat tagcagcaat atcaggatta
     4441 ggaacaagtt caatgaccat accctctctt tcttgccgtt ttatcttgct tttttctttc
     4501 tctttaggct taaaatcagc aactgcagct gccataatag ctgcatcgca aactgaaaaa
     4561 tacttcaagc attgctcaag catttcgtct gcagaaacta cactaatgac ctttatgttc
     4621 ttatgtacag tctttacagc aacaggtccg ctaatcaaaa atacttcagc cccttcattg
     4681 gcacattctt cagccaaggc ataacccatc tttccactac tatagttgcc aataaacctc
     4741 acagggtcaa tattttcaaa agtcgggccg gcagttacca atatcttttt accgtcaagt
     4801 cttttggcag tttcaaaaaa ggcagaaatt gctgcaacaa tcttttcagg ttcttccatt
     4861 ctaccctttc cttcaagccc gcttgccaac tcaccggtag aaggttctat aatctttact
     4921 ccttgattag ccaatgtttt aagattattt tgagtggtaa catgggcaaa catatccaaa
     4981 tccatagccg gagcaacaaa aacctgacac ctggccgaca aataggtagt cagcaaaagg
     5041 ttatcagcaa taccattggc cattttacca atagtattgg cagttgcagg cgctataaca
     5101 taggcatctg cccacatgcc aatatccaca tgactattcc attgtccatt ggtagggtca
     5161 aaaaaatcaa ccaaaatgga atgttttgac aatgttgcta aggtgagggg ggtaataaac
     5221 tctttagcca acggggtcat tagtaccctg acatcagcac cctctttaac aagcaaccga
     5281 actaaatttg cggctttgta tgctgcaatg cttccggtaa taccaagtaa tatgttttta
     5341 ccttttagca tagcttacag cgctaagtta ttctttcttc ccggttttct tgaaaaaaat
     5401 ctcgttatcc tcgtactctt gcaaagctat aagagtaggt ttaggaagtc tttcatagaa
     5461 ttttgaaact tcaatttgtt cacgattttc aaaaatttcc tctaaattat cgttcgagtt
     5521 accatattcc tgaagtttcc tgttgagttc atcttttatc tcaacagata tttgattggc
     5581 tcttttatat aacactacta ctgtttcgta tatattgcca gtaacagact ctaacttgtc
     5641 caaatctctg gtaattgtag atgaaggagc tttgcttttt ttgtaatcag atgccatgtt
     5701 atttatttta ttataaacct aatacttctt ttgctttatt aaaaatttta gttacctgtt
     5761 tgtcatactg ccctttagga tattcggcaa catagctgta atattcgtcc aatgcatttt
     5821 gaaaacgttc tttctgtttt tctgttatgc tgtttaaagc cagttcatac tttgcatcca
     5881 gcaacataaa catcagtttt tcccgatggc tgctttccgg atattgcgtc aaacaattat
     5941 tcaaagctat aatagcagct ttgtaatatt ttaaatcata gtacaatttt gcattaagat
     6001 atgctttttc tgccagttta tcttgcaatt cttttatcag ccgcttacac tcaggagtat
     6061 atttgctcga aggatgttta taaataaatt tctgaaatgc agctacagca aggtttgtat
     6121 tctcctggtc taaatttgct cttggcgaaa ccaaatagta gcaatatcct accatataat
     6181 cggcttcttc aacaaaaggg ctgcgggcat agttgtccga taattcttta aaataaaacc
     6241 cagccatagt atagtcttca tcaccataat agctttgagc aaacagatac attaaagaat
     6301 ctccttttgt cgaagcccta aaataactga ctatctgttc aaaaatggta atggcacggg
     6361 cgtaatcttt cttctcatag tattgaaccg ctttgcgata tttaaaatca taatcgttgc
     6421 ttttcaatac cttttcatat tcactacaag acactataat taaagtactt acagctacta
     6481 aaaatattgt taaaaggcta tttttttgca tggcgcaaat ttacacttta aattaaaaat
     6541 aaaaaaaatt cccaaagcaa aaaacgtaaa taaaatcaac ttgttttgct tgtatagtgt
     6601 taaaaaaaca aaatgaatgg ctgttaaccc caaattaatc attaggaggc cttttaaagg
     6661 cgaattatat gatttagtta ggttaatttg agtttatcaa gtttatcctg tactaacaca
     6721 aaagccccgc taaatgcggg gcttttgtac ccggagccgg aatcgaaccg gcacaagatt
     6781 gctcttactg gtttttgaga ccagcgcgtc tacctattcc gccatccggg cagggacact
     6841 tttatttggg actgcaaaaa tatcaaatag tttgatatgc acaaaactta tgcaaacttt
     6901 ttagttattc tttacacaaa gggcatcatt tagcgctgca atagccttat tataatgctc
     6961 gcgctcaata acaaactgaa tgcttacctg gcttaatgat tgcgaaatac aatcaatatt
     7021 gatattgttt tgggacaaag ctgttgttgc ttttgccaaa atgcctggtt tggcaatgtt
     7081 tgtacctaat gtacatacta aagctacatt ctttacagta attttatgga aacttttttg
     7141 taaatcagca ataaattctg ggtaaacttc atcttgccaa attactgttg ttatactgtt
     7201 ggcatttgac gatttaagaa tgtaactaat gttgtatttc ttaaaaaatt ccataatggc
     7261 ataatcaaac ccaacttctc caaccataaa cggatcgtga acttctatag ctgttacttt
     7321 atctgagcct gtaacaatct ctatcttaga ttctttactt atgtaatctt ttgagataag
     7381 cgtccccgga tgctcaggtt caaaggtgtt tttgatacga atgttgatac ctgctatctc
     7441 aattggtttg gatgctttag ggtgtatagc ctccatcccc acatctgcaa gctgatcagc
     7501 aacattatag ttggtaaaac ctaccggaat agatttctca acgcctacta ttttaggatc
     7561 ggcagatgat aaatgaaatt ctttatgaat gatagcttca tcagctttta cttcaactgc
     7621 aattttgcta aaagttactt ctgagtatcc acggtcaaat tcacgcataa taccttcggt
     7681 cccttttgta tatcctgtag ctatacaaag cactttagaa aagtctatac cttgaaaagc
     7741 tttgtggata cgttcgtcta tagtcagata ttcagggtcg ttgaacccac ataaatcaat
     7801 aaacttagca tttacgccac gattgttcaa aatgttgaca gagttaaagc cagaatgagc
     7861 ttctccaata gaagccaata tttcgcgggc tgcaagcagg atgctttgac gtgatacata
     7921 gcccgtagca agtacatctg ccagactgtt tagatagttg cgggtttgga caattcgggt
     7981 gctgataaaa tgatcggctg ctttaaggtc aagcccaatg ttttcaaaac tatgattaat
     8041 ctcaataagt tttttgagca aatcggcaag cgcttcattg taatcacggc cttcagcaaa
     8101 acgagcgtaa ataccaggct ctttagtctt tttatgttcc aaaagccagt tggttacatt
     8161 gttgtatgcc gaaacaacca tgatacggtt ataataatca ccttctttgc ggctgccaat
     8221 gatgatattt tttacaacat caccaaattg agacattgaa gtgccaccaa ttttttctac
     8281 tgtcaacata ttttttgtcg ttttggcgca aaaatagttt ttttagtgaa aaaactattg
     8341 ttttatatta aaaagggaat aaataattct gtttttgcca ttgttttcta ataacattct
     8401 caatgttttg taaaacaaaa ggctatgtat aaaaaagtta agaaacaaaa cgcaaaggtg
     8461 ggaagattat tggcaaagaa cgcaaaatgc caataatcaa ggtttaatat attttattct
     8521 ttcttcgtat atcttgaggt ttatatttcg tttacacacc cgctttatta ccattcgggg
     8581 ggtatcgggc ttttaaaatt tattttgttc ggcgaacaaa agacaaattg caagcaagaa
     8641 aaattcccta ttgggcattt taagcgctat actaacttat ttacaacggg tttttctttg
     8701 actttcgtct tttcgcccaa gtacatcttt tatatactta acatcatccc ttatagcatc
     8761 aatagtctgg gcaagctgat ctttaggctc cataggcccc gggagctctg aagaaatata
     8821 gttgacaaac tgccatattt cgcgtatttg tgatacatgc aaatcgtaag gctgatatat
     8881 tgggttaagc gaataaagtc gaagtttatt ttcttcactg ataaggtttt cagcaatttt
     8941 aaaaactact ccttcatcaa gggtaaggac tatatatgca tggccactga ttatgctacg
     9001 ccaatcttgt ataaacaccc ccgttatcca tgctttatcg ggaatgggga gcattgagtc
     9061 tcctgttatc tgaaatgtcc ggtatttctt ttgtttcgaa agaaaaggca actgaaatac
     9121 aggcaattcc tggatatact cagggtctgc aaaacctgtg gcatagccag ccttagcttt
     9181 ttctgaaaca agttctatat tatcttcgtt ttctttattt acagttgtag ccaatactct
     9241 aagcttagta cccgacagat atgcatcgtt tccccgttct aattcgccaa gctgaaactc
     9301 ggacaacttg cttaaatcta cttttaccag gctgtctata gatatattga aatatttgga
     9361 gcaagctatc aacgtatcta tggatggttc ggccacacca ttttcatagc cattatatgt
     9421 aggcctcttt atccctaatg cagctgaaac atcttcctgg gtgcgacgtt tacgcttacg
     9481 caaaaattta agattagaag tgaaatacat attttttatc tttttacaat atttacaact
     9541 ttcacataca aagacttata catcttttta agccataaag gtatagcaaa aagattaaaa
     9601 atacaacaat agtaaagatt tattactaat caatttcaaa ataaagtatt ggctaataac
     9661 caacaaccca aagaatccgg ccttcgtcaa tacacctttc aacaatggca atcattttct
     9721 tattgacatc ttttagttgt tcgaggtatg aagcccagtt gtctttccag tgaacagagt
     9781 ccgactcaag ctttttaaaa aactcttttc ccagaaaaag actattaata aaggtttcta
     9841 acgtcatgag gctatcaata gagttgagct tatccagaaa agaaatgagt tcgttgcgca
     9901 ggacaacaat tttgcggcta ttgaaccggg ttggctcaaa atattcataa agctcattgc
     9961 tattttcaaa acaagaaaga aaaagattga acacctcact tccaagataa gctgactctt
    10021 cctgtaaagg cataaaagta tcgttatccc cggtgatatt gaattctaca aaattaagct
    10081 cgtttaagct aaaaaaatta agcctcatgg tttctgttgt aaaaattctt tgataaaaat
    10141 agaaaaaaat atgagaaacg cacatctggt cggtagaaaa tctcgatata tagttatttt
    10201 tgtacaaagt ataaaaaaga gaaatgacag accacataac cgatataaaa caattgtaca
    10261 tgcagaattt cgggaaagag cccgaaaaca ttgttgcatt gcctccatca ggctctaatc
    10321 ggaagtatta ccgtatttca aatgggaacg acagttgcat cggggccttc aacgaggatt
    10381 ttaaagaaaa taaagcattt ataaacttta caaaaatctt tcttcaccat gagctccctg
    10441 tacctaaaat tatagacgaa aaccttgaca aacatattta cctgcttgag gatttaggca
    10501 ataacaccct tcttagcctc atagaaggca acttagacaa agggttacaa cctgaaaaca
    10561 ttgctttgta taaaaaagta ctggattacc ttcctatatt tcaggttgta gcagggaaag
    10621 acatagacta ctccgtatgt taccctcgaa atgcctttga caaacaatcg atgatgtggg
    10681 acctcaacta tttcaagtat tattttctta agatggccca gatacctttt gatgagcagc
    10741 ttcttgaaat tgattttgag tgtttctgcg actacctgct gcaaaccgac tgtaaccatt
    10801 tcctgtaccg cgactttcag tcacgtaacg tgatgataaa agacggggag ccttatttta
    10861 ttgactatca gggaggacga aaaggggctt tacaatacga catagcatct atgctgtttg
    10921 aagccaaaac tcaccttaaa ccagaggtta gacaagagct ttatgaatat tatatttctg
    10981 tactaaagaa ataccatagc tttgacgaag aaaagtttac cagccattac tatgcctaca
    11041 tatacatccg cttgatgcag gccatggggg cttatggttt ccgagggctt tatgagcgca
    11101 aagagctttt cctgcaaagt attcctaaag cattggaaca cctgcgctgg ctcagggaaa
    11161 atgtaaagct cccgcttaat atgcctcaac tggaacgtgt gtgggacttc ctggttgaaa
    11221 acgagcatat ccgcaacctg gcaaaaacgc ccaattacct tacagtacat atcagcagtt
    11281 tttcgtacaa agaaggaatc ccatcggaca gctctgttca tggcggaggc tttgtgtttg
    11341 actgccgtgc cctcgaaaac cccggcaggt atgatgagta caaaagcctt accggcaaag
    11401 atgctgaggt gataaagttt ttagaatcaa aaagtgatgt acagactttt tttgcgcatg
    11461 tttcgagcat cataagccaa agcgtcacta cttatcaaaa aagggagttt gactacctgg
    11521 ttgtaaactt tgggtgcacc ggcggacagc accgctcagt atatatggcc gaaaagcttg
    11581 cccaatacct gaaaaagcag ttcagccata taaaaataga ccttcaacac tgtcaactcg
    11641 acaaaataaa agcacaatga aggccatgat atttgcagca ggccttggca cccgcctgcg
    11701 cccattgaca gacaacaaac caaaagccct ggttgagatt aatggcaaaa cactgctcga
    11761 aattaccatt caaaagctta aagcagcagg ttttaatagt attgtagtca acgttcacca
    11821 cttttcaggc cttgttattg acttcttgaa aaagaacgac aactttggcc ttgatattga
    11881 aatatccgac gaaaccgacc tgctgctcga cactggtggc ggattaaaaa aagccgcccc
    11941 gctcctcggg actcaagaac ccgtattgat ccacaatgtg gacattgtaa gcaacattga
    12001 tattggagag ctatacacac aacattgcca gtcatcagca ttggcaaccc ttgcaacaat
    12061 ggaacgggac tcgtcaaggc aattgctggt caacagcaaa gggatacttt gtggatggca
    12121 aaacaaatct acaggagaaa agaaaatttc tcaaccaagc gaaacagatt taacaccaat
    12181 ttcttattgt ggcattgctg tccttaatcc tcagtttctg acattgattg aagagaatgg
    12241 tgttttttca ataatagatg tatatttacg ccttgcaaaa aattttgaca ttcaaactgc
    12301 aaaccaggaa aacatcaatt ggagcgatgt tggtacggtc gagagtttga aaagattgag
    12361 gagaaatgta aattaaaata aatgaaatta tcaataataa taccactcta taatgaagaa
    12421 aggtatatac tcagcacttt aaaaaaagtt gaagaggtaa taatacctaa aagcattaat
    12481 gacgttgaaa tcatagtgat tgatgattgt tcaaaagata attcctggca aattataaat
    12541 gaatatgtca aaaacaagga aaatgtcaaa ttattcaggc atgatgtaaa caaaggtaaa
    12601 ggagctgcaa tgagaaccgg aattgctaat tgtacgggag acttagtggt ttttcaggat
    12661 gcagatttag agctttctcc ggaagatatt ccagtaatga tagatacaat gataaaactg
    12721 gatgttgatt ttgtaaatgg ctccagatat ctttctggtg tacctcgccc cctatcttct
    12781 ttttggagat atatcggaaa taaaattttt acattattaa ctgctatgat tattaatgta
    12841 aaaatttctg acatggcatg tggatacaaa ctggtgaaaa aagatttatt agacaaactt
    12901 aaccttaaag aaaataggtt tggaattgaa gcagaaataa tgatcaaagc tcttcggttt
    12961 aaaaaagaaa gaatcgcaga agtacctgta cagtatctcc caagaacaca acaagaaggg
    13021 aaaaaaataa gaaattctga tgcatttaaa atattttata aaatacttaa atatggcctt
    13081 tttaaagctt cgtaataaag tcaaaacatg gagtattagt ttaatactag tatttttatc
    13141 tattttttta atttcaaatg tttcaaaaaa catctcgctt tataataaac aagaagctgt
    13201 tattgaatgg gatgcaattt cattatatgc atatttacct gcattcttta tttataatga
    13261 tattaagctt gattttgtgc aacccaatgg aaaaactgga gattgctcat atacaatatg
    13321 gtattcagaa aacaataaag gtcaaagaat tattaaaaca tctatgggag tatcttacat
    13381 gtatttgcca ttttttcttg gagctcattg gtactcatcg attacaaata atccgacatg
    13441 cggatactcc cctatttata aaattgggat tattataagt tgtttatttt atctttttct
    13501 tgggctgttc tttttaagaa aaattctcct aaaatactat tcggagcttg tgacaagtat
    13561 tgttttgatt atattgttgg ctggaacaag cctatcatat tattccattt ttctacctgc
    13621 ttatactcac atttttagtt ttgcgttagt tactatgttt ttatattttt caatcaaatg
    13681 gaatgaggcc ccaagattca cttatagtat cgctttaggg ctattgatag gagttatcac
    13741 cttgataagg ccaactaact ttcttattgt tttagtttgg atcttataca atatttataa
    13801 tttaaacagc ttaaaactaa ggataaaatt cttctttact cactattcta aaattctatt
    13861 aattgctttt cttgcattct taacttggat tcctcaattg atttattgga aatatgttac
    13921 cggagacttt ttttactatt catatggaga aaatgaaaga ttctttttta accatcctca
    13981 tattattgaa ggtttatttg gtttcagaaa aggctttttt atttacacac ctatcatgat
    14041 ttttgctttt attggtttat attattattt caaacaaaat aaagcattag ctattcctat
    14101 cattatttat tcattattaa caatttatgt tacactttct tggtggtgtt ggtggtatgg
    14161 aggttgtttg ggcatgaggc cttttattga ttcatacgca atatggtgtt tacctttggc
    14221 agcgttaatc aaccaattaa attcgcagaa aattggttgg attattggca tttatttatc
    14281 catgtcaatt tttatttggt tcaactttat gtatatgaaa aaataccgta caggaggagt
    14341 tcattt
//
LOCUS       DAOSPF010000075        13809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4461_length_13809_cov_7.202414, whole genome shotgun sequence.
ACCESSION   DAOSPF010000075 DAOSPF010000000
VERSION     DAOSPF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4461_length_13809_cov_7.202414"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PK252_12285"
     CDS             <1..730
                     /locus_tag="PK252_12285"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT15530.1"
                     /translation="KTVIVTGAARGIGKCIAQTYAGKGANVVIADKDKVEGKKTERKI
                     LKAGYKAVFIETDVSNPHDVYELVHLSCGKYGNIDILINNAGISRFTPIFELTVEAWD
                     EIINTNLRGTFLCSREVAKIMRITGKGSIVNIASTRAFMSEPDSEAYAASKGGIVSLT
                     HALAASLAKDHIQVNCISPGWIETGNYALLTKDDHSQHFAGRVGKPEDIANACLYLTS
                     DGNDFISGTNIVIDGGMTKKMIYL"
     gene            complement(736..1956)
                     /locus_tag="PK252_12290"
     CDS             complement(736..1956)
                     /locus_tag="PK252_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15531.1"
                     /translation="MNKVIIILSLACLTLAQCSSKTNAFSALEKGKVLSRVCAGSTEN
                     SYAVYIPKSAEEKPLPVIVCFDPKGDGTLPVNLFRMTAEKYKVMVVGSNVIKNGMPMQ
                     DVNYFANELFKEIRQNMPVDSSQFYLAGFSGGSRVALYLATASVVPVKGVIACSAGGE
                     ALRLNKNIVVAALTGDSDMNYLEMKQFADQLGMYGISRVFYSLPSVHQWPDSNSLANA
                     FADIVVMGMANNNVPMDSVFVKQHYLFYNQQINALSKSFNIKDSLKKAVSIAANGARV
                     FRNFTNFNKIELFRDSISSLPTVKALFVEAEKMEQAEKSGQEKIYQLYVSGHYNELLN
                     LLDKLAKEKKQNIVASRLLAYCSLMSYSYVGRALQANDLTLAKPLLEIYHRCDPQNKD
                     YARFLERYKELGGQ"
     gene            complement(1953..2702)
                     /gene="bla"
                     /locus_tag="PK252_12295"
     CDS             complement(1953..2702)
                     /gene="bla"
                     /locus_tag="PK252_12295"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HOT15532.1"
                     /translation="MRKIRFFLYCFISISVSVLGQSNYKSIKLSPDIELVKISENAYM
                     HVSYAFMPGFGRVGSNGLIFINEKEALLFDTPGNDSLTQVLTSWICDTMQFRLVGFVP
                     NHWHDDCMGGLKYLQSIGIKTYANQMTINIAKQKNLPVPEIGFTDSLELRLGKKLVKC
                     YYFGAAHSADNIVVWVPSEQILFAGCMAKAISSQNLGNTADGDVNAYPATIKKVYNKF
                     GSAKVVIPGHGSLGGLELLTHTIDLAKNIKK"
     gene            complement(2714..3226)
                     /gene="idi"
                     /locus_tag="PK252_12300"
     CDS             complement(2714..3226)
                     /gene="idi"
                     /locus_tag="PK252_12300"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOT15533.1"
                     /translation="MSKTYVILVDSNDNQVGVEEKMAAHEKALLHRAVSVFIINSKGE
                     WLLQQRAMDKYHSKGLWTNTCCSHPFPGESTFDAAARRLQEEMGLKAGIKEVFSFLYR
                     EDLDNGLTEHELDHVFVGYSDELPVPNADEVMAWKYIAFDDLKTDISSHPDNYTVWFK
                     MIFEKVNKHL"
     gene            complement(3368..4168)
                     /locus_tag="PK252_12305"
     CDS             complement(3368..4168)
                     /locus_tag="PK252_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT15534.1"
                     /translation="MQERWIFQRTERLFGKEKMEQLASKRVIIFGIGGVGSWCAESLV
                     RTGIKHLTIVDSDRICTTNINRQLHATTQTVGLVKVDVLKQRLLEINPHAEITAIQKI
                     YNRANHPSFELGTYDFIIDAIDSLGPKMELIRKATRTNACFFSSMGAALKIDPTRIKV
                     GEFWSVNGCPLASIIRKKIKKGDLPAKEFLCVYSDEVLENKGGHLHCSNEMNDCPKKD
                     IVGDPELADHEWTNYKASVNGTTAHIVAIFGFTLAGLVIKAIYNEPVE"
     gene            complement(4518..7364)
                     /locus_tag="PK252_12310"
     CDS             complement(4518..7364)
                     /locus_tag="PK252_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15535.1"
                     /translation="MKLLFIYSFLFIFFCNAHAQLTGMGQDPSSVKWRQIQTKRFRLV
                     YDTCYENQAQKVAAIMDYSLSISGNTINQNISNTPILLHNHNAISNGFVTVMPNRMEF
                     YTAPPQDMPAIGWFEMLGIHEGRHQGQMLKMNTSYLGYSKYLFGEGLWGLNAALQAPS
                     WFYEGDAVAAETALTNSGRGRLPSFVMATRARLIGTHRPFHFRKAWHGSYKDYVPNQY
                     EFGYLMVAYNRIKYGTGLWETVLQEIADSPFPGITAPFLDAVKNKTSKNRRQLYNETY
                     KALDSIWTAESHHLSLTSFDKITQKPFSYTNYTSPVVAEGKLVVYRRGLSYTPAVVSI
                     DKNTGEEKKLFEPGYILSEKISAWRNWIYWDEYRPDIRWANRSYSVIMAYNMATGKKI
                     QLTQKSRYYAPAVSPDGEKLIVVENDVKGMNSLVILDANTGEKLRSFTFGQKAVQSPC
                     WSPDGSSIAYTMVADNAKGLYCVDIQSGKVDTLILPGFHQVEKPFWSENTIYFSASYS
                     GIDNIYAFNVNNKKVYQVTSALNGAFDACVSQDTLIYANYTMNGMEIVQSVIDTVKWT
                     EKKVVDTYQMALADKLSAQEPQKYNPDSVRYIRYDSRPYRKIGHLFHIHSWVPLYANS
                     SATTLDRLLMPGYTVANQNLLNTFVFTGGQGFWQGKLYSSLHVTWQGWFPVFQAGLDY
                     GDSVKMYGLSSDYRSSASKFIVYTNVSIPLSTVNGQYGFSLAPGATYSYDNRLYSARN
                     GVIFKGAHNSVFSVNASVTKNSAARDLFPMLGVKTEFIYVQPISPSNVFSPQQVGSVK
                     VFLPGLYYHHSLRISVAYEEQQLNNYFQPDRLPAPRGYEILWDTATPSFTTMKRWAID
                     YSLPLVYPDINILKVFYLKRIWFTGFHENSQAKVYKNKAWQSRNYVSNGGELYFDFRA
                     FSLPPTLRIGYRLSFVPSEKRSEAMIVWGYIADF"
     gene            7543..8040
                     /locus_tag="PK252_12315"
     CDS             7543..8040
                     /locus_tag="PK252_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT15536.1"
                     /translation="MAVLYHIQHLINIIVDWLYRPFKRFVPKETFKYAVCGGANTAFD
                     IFLYFITYNFILQKKIINLKLIAISPHIASFLLVFPITFSTGFLLSKYITFTSSTLRG
                     KTQLLRYGFTVLVCIFLNYILLKLFVEGFGWYPTPSKIATTCLVVIYSYFSQRHFTFK
                     TQQAV"
     gene            complement(8037..8948)
                     /locus_tag="PK252_12320"
     CDS             complement(8037..8948)
                     /locus_tag="PK252_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOT15537.1"
                     /translation="MKVLISPSNNPYFNIAAEEYLLHHSNEGLLFIYINNDAVVVGKH
                     QNTLAEINYRYIIENNIPVIRRISGGGTVFHDLGNLNFAFFSVTEEGKQIDFEKFSKP
                     ILDALYESGIKAYREGKNDLRSAEFKISGNAGHVYKNRAIHHGTLLVEANLERLKDCL
                     KVKPKVYIDKAVQSIRSSVTNLRDIYHSISITELKDKIVRSVDGLIVEPEADFDKAVN
                     SLVQSKFQTWEWNYAYSPAYTFVSYDEHDVYFKLNIEQGIINKVEIINKNYAWAKTLI
                     GIRHQYLNVSEALTNEGLKADTVWQCF"
     gene            complement(8932..9570)
                     /locus_tag="PK252_12325"
     CDS             complement(8932..9570)
                     /locus_tag="PK252_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT15538.1"
                     /translation="MGNIEWTIGYYMLETINKLDISILLGINGINSPWLDVFMWFVSK
                     TFVWVPFYLLLAYFIVRKYKKDGIWFLLAIGLSIVLSDQLSSGLIKPLAERLRPSHNP
                     EIAEMLHYVNDYKGGKYGFVSSHAANSFALAFLVSFLFHRRWVFFSIYGWAAIVSLSR
                     IYLGVHYLTDVLCGAIVGIFSASTVLFLFLKYIPALTKYRDLKPVAQDESSD"
     gene            complement(9546..10538)
                     /gene="obgE"
                     /locus_tag="PK252_12330"
     CDS             complement(9546..10538)
                     /gene="obgE"
                     /locus_tag="PK252_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT15539.1"
                     /translation="MNENFVDYVKIFVRSGKGGAGSAHLHREKFVAKGGPDGGDGGRG
                     GHVIVRGNSQLWTLLHLKYTKHIHAENGQNGGSAQSFGAFGEDKYIEVPLGTVAKNAE
                     TDEILFEIIEHGQEEILAKGGRGGLGNVHFKSATNQTPRYAQPGEPGIEGWFILELKV
                     LADVGLVGFPNAGKSTLLSSVSAAKPKIADYPFTTLVPNLGIVKYYGHKSFVMADIPG
                     IIEGAAEGRGLGLRFLRHIERNSVLLFVISADSKDIAQEYNILLNELEQYNPELLDKQ
                     RLLAISKIDLVTEENIKKLKSQIPKGIKCTFISAVTNKGIAELKDMLWETLNGQ"
     gene            complement(10651..11220)
                     /locus_tag="PK252_12335"
     CDS             complement(10651..11220)
                     /locus_tag="PK252_12335"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT15540.1"
                     /translation="MLNIVLFGPPGCGKGTQSEKLIEKYQLVHLSTGDILRAEIANNT
                     KLGIEAKAFMDKGQLVPDQVVIGMIASKIDNNKNAKGFIFDGFPRTTAQAEALDVMLA
                     ERNIPVKCMLSLEVDKEELVKRLLNRGLTSGRADDQNPEIIENRIKEYNAKTAPVIQF
                     YEKQGKFRPIKGMGSIDEIFERLCKAIEK"
     gene            complement(11253..11909)
                     /locus_tag="PK252_12340"
     CDS             complement(11253..11909)
                     /locus_tag="PK252_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT15541.1"
                     /translation="MIEFLKFALDFILHIDVHLKEIVSQYDTWTYLILFCIIFCETGL
                     VVTPFLPGDSLLFAAGALAAGAGAPLSIFTLITVLMIAAIAGDNSNYFIGRFLGHKIY
                     EKNFRLIKREYIDKTHKFYEKHGGKTVVIARFMPILRTFAPFVAGIGEMKYLRFFLFS
                     IGGTILWVNTFCWAGYLFANNSFVQKNFTIVVFAIIAISLIPPVLTVVKQWLGKRKNK
                     "
     gene            complement(11971..13050)
                     /gene="carA"
                     /locus_tag="PK252_12345"
     CDS             complement(11971..13050)
                     /gene="carA"
                     /locus_tag="PK252_12345"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOT15542.1"
                     /translation="MEQTKPVKLVLEDGTEMQGFSFGYEESVSGEVVFSTSMVGYPES
                     LTDPSYKGQILVLTYPLIGNYGVPGIEMEENLPKYYESDRLHISGLIISDYSIEHNHW
                     NAQMSLSDWLKQYKVPGIYGIDTRALTKRLREKGTMLGKIVVDNKDVPYFDPNKVNLV
                     DMVSTKERIVYGNGKYKVLLIDTGVKYNIIRNLLKRDVTLVRVPWDYDIFSEEFDGLF
                     ISNGPGDPKMCTTTIKNISKAFEMDKPIMGICLGNQLMALAAGADTYKLKYGHRSHNQ
                     PVLQVGTNKAYITSQNHGFAINNNTLPKDWEPLFINLNDNTNEGMRHKSKPFFSSQFH
                     PEATGGPVDTEYLFDDFIENIKKTK"
     gene            complement(13064..>13809)
                     /locus_tag="PK252_12350"
     CDS             complement(13064..>13809)
                     /locus_tag="PK252_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT15543.1"
                     /translation="PLPDSIQKEVDMLIAKSRTSPELFRYMLVTLFSYYNKSQIMGYD
                     DVAIYIADKYYINEATWADSAQLKKLKKQVAEKKPTMLGKMSPAIDLFEVPPSHFMIA
                     KEDTFEKHNLYIGTPFSMHARMKDYTILYFWDVDCGHCKKETPIMYEVFQNLKSKNVE
                     VIAVCLVYNRDHKSKWVDFVNEHNMYDWINCFNPFTLKHKELYDVQSTPSIFVLDKQG
                     KILAKRLGAKQCEDLINHEISLSQKKNNK"
BASE COUNT         4046 a         2817 c         2564 g         4382 t
ORIGIN      
        1 taaaacggtt attgttaccg gtgctgcaag ggggataggt aaatgcattg cccaaacata
       61 tgctggtaaa ggggctaatg ttgttattgc agataaggac aaagttgaag gcaagaaaac
      121 agaaagaaaa attctgaaag ccggatataa agctgttttt atcgagactg atgtaagcaa
      181 ccctcatgat gtttatgaac tggtacatct ttcgtgcgga aagtacggaa acattgatat
      241 tttgattaac aatgccggca tatcacgctt tactcctata tttgaactga ctgttgaagc
      301 ttgggacgag attatcaata ccaaccttcg gggaacgttt ctgtgttcac gcgaagtggc
      361 caaaataatg cggataaccg ggaaaggctc cattgtaaat attgcttcta cacgagcctt
      421 tatgtctgaa ccggattcag aggcttatgc agcatctaaa ggcgggattg tatctttgac
      481 gcatgccttg gccgcttcac ttgccaaaga ccatatacag gtcaattgta tcagccctgg
      541 ctggattgaa acaggcaatt acgctttgct gacgaaagat gatcacagcc agcactttgc
      601 aggccgtgta ggcaagcctg aagatatagc aaatgcttgt ctttacttaa cttcagatgg
      661 caatgacttt atttcgggta caaatattgt tattgatgga ggaatgacaa agaagatgat
      721 atatttgtga gctttttatt gtcctcccag ctctttgtac cgttccaaaa accttgcata
      781 atccttgttt tgtggatcac aacgatgata aatttccaac aaaggttttg caagcgttaa
      841 atcgttagct tgcaatgccc tgccaacata gctgtatgac ataaggctac aatatgccaa
      901 cagccttgac gcaacaatgt tttgcttttt ctctttagct aatttgtcaa gcagattgag
      961 cagttcgtta taatgacccg aaacatacaa ttgataaatc ttttcctgtc ctgatttttc
     1021 agcttgttcc atcttttctg cctcaacaaa taaggctttg acagtaggca aactgctgat
     1081 agaatcgcga aacaactcta ttttattaaa attcgtgaaa tttctgaata cccttgcccc
     1141 attggctgca atactgacag cttttttcaa actatctttt atattaaaac tcttggaaag
     1201 agcattgatt tgttggttgt aaaataaata atgctgtttt acaaaaactg aatccatggg
     1261 aacgttgtta tttgccatcc ccatcacaac aatatctgca aaggcattag ccaggctatt
     1321 actgtcaggc cattggtgaa ccgatggtaa tgaataaaac acgcggctga tgccgtacat
     1381 gccgagttgg tcggcaaatt gcttcatttc gaggtaattc atgtctgaat ctccggtaag
     1441 agctgcaaca acaatgtttt tgttgaggcg taaagcctca ccccctgccg aacatgctat
     1501 tacaccttta acaggaacaa ctgatgcagt agcaagatat aaagctaccc ttgagcctcc
     1561 tgaaaaccct gccaaataaa actgcgacga atcaactggc atgttttgcc tgatttcttt
     1621 gaatagttca ttggcaaagt aattgacatc ctgcatgggc atgccatttt taatcacatt
     1681 agagccaaca accattactt tgtacttttc tgcagtcatc ctgaaaaggt ttactggcaa
     1741 agtgccgtct cctttgggat caaagcaaac aataacaggc aatggctttt cttcagccga
     1801 tttgggaata tatacagcat agctgttttc ggtactgccg gcacaaaccc tgcttaacac
     1861 cttccccttt tcgagggccg aaaaagcatt agttttactg gaacattggg ccagagtcag
     1921 acacgcaagt gaaagtataa tgattacttt gttcattttt taatattttt tgccaaatca
     1981 attgtgtgcg tgagcaattc gaggccgcct aatgatccat gcccgggaat aacaaccttt
     2041 gccgacccaa atttgttata aaccttttta atggtagccg gataagcatt aacatcgcca
     2101 tcggcagtgt tgccaagatt ctgtgagctt attgcttttg ccatacatcc tgcaaaaagt
     2161 atttgctcag atgggaccca cactactatg ttgtccgctg aatgtgctgc cccgaagtaa
     2221 taacatttta caagcttttt gccaaggcgc aattctaaag agtctgtaaa gccaatctca
     2281 ggaacaggca gattcttctg tttagcaata ttaatggtca tttggttggc ataggtttta
     2341 atacctattg attgaaggta ttttagacct cccatgcagt cgtcatgcca atgattaggg
     2401 acaaatccaa cgagcctgaa ttgcattgtg tcgcaaatcc aggatgttaa aacttgtgta
     2461 agcgaatcgt tgcctggcgt gtcaaaaaga agcgcttcct tttcattgat aaaaatgagg
     2521 ccatttgacc caaccctgcc aaagcctggc ataaaggcat aactaacgtg catataggcg
     2581 ttttcggaga ttttcaccag ttcgatatcc ggcgaaagtt tgattgattt ataatttgac
     2641 tgccccaata cactgacact aatgcttatg aagcaataca ggaagaagcg aatttttctc
     2701 attttttata ggattacaag tgtttgttta ctttttcaaa aatcatttta aaccaaaccg
     2761 tgtagttgtc cggatgggag gagatatccg ttttcaaatc gtcaaaagca atatatttcc
     2821 aagccatcac ttcatcggca ttgggtactg gcaattcgtc cgagtaacca acaaaaacat
     2881 ggtccagttc atgctcagta aggccgttgt ctaaatcttc gcggtagagg aaagaaaata
     2941 cctcttttat tccggctttg agccccattt cttcctgaag ccttcgtgct gcagcatcga
     3001 aagttgattc gcccggaaac gggtggctgc aacaagtatt ggtccacagg cctttagagt
     3061 ggtacttgtc catagcacgt tgctgcaaaa gccattctcc cttgctgttt ataataaata
     3121 cagaaactgc ccgatgtaaa agggcttttt catgtgctgc cattttttct tcaacaccaa
     3181 cctggttgtc gttgctatct acaagaataa cgtacgtttt gctcatattc tttttgtgat
     3241 gagatacaaa aataggctaa atagttggca aattattcgt tgttgaaagt ttggttaaca
     3301 caaattgtcc tcacttttta tcgtaagggt ttagggattt atgcagtttt tcgaattatt
     3361 caaaaatcta ttctacaggt tcattgtaga tagcctttat caccaacccc gccaaggtaa
     3421 aaccaaagat tgctacaata tgagctgttg taccatttac cgaagctttg taattggtcc
     3481 actcgtggtc ggccagttcc gggtcaccta caatatcctt ctttggacag tcattcattt
     3541 cattgctgca atgcagatga ccacctttat tttcaagaac ctcgtcacta tacacacata
     3601 aaaattcttt cgccggaagg tcgccctttt ttattttttt acgaatgata gaagccaacg
     3661 gacagccgtt gacactccaa aattcgccta ctttaattcg tgtagggtct attttcaggg
     3721 cagctcccat agaagaaaaa aagcaagcat tggtacgcgt ggctttgcgt atgagttcca
     3781 ttttcgggcc aagactgtca atggcatcaa tgataaaatc atacgtaccc agctcaaacg
     3841 aagggtggtt ggcacggtta taaatcttct ggatagcagt aatttcggca tgaggattga
     3901 tttccagcaa tcgttgtttc agaacatcca cttttaccaa gcctacagtt tgggttgttg
     3961 catgtagctg ccgattgata ttggtggtgc aaatacggtc cgagtcaaca atggtaaggt
     4021 gcttgatgcc cgtacgcaca aggctttcag cacaccagct gcctacaccg ccgataccaa
     4081 aaataattac tcgtttggag gcgagctgtt ccatcttttc tttcccaaac aaccgctcgg
     4141 tacgttgaaa tatccatcgt tcttgcatgc ctttgaaaat gtcaattgat atgagaaaaa
     4201 ttacaaaaga atcaaatttt gttgtccctg aataagaatc tcatattttt gttttatccg
     4261 tataaagttt aatttattga attttaatta tttatactgt caatagctgc catttaatgt
     4321 gagccgattg aacatccagt tgtccatttt atttatccct atagctgtcg ggatgtttgt
     4381 agtttgcaaa atatggatgt ttcaaggcaa aagtatgaaa aatttaaagc ttattgtaat
     4441 tggccaaaag ctatgttcat gttttaggta caacaatata ttttgcctat agttgacctc
     4501 gcttttttgt caaactttta gaaatctgca atgtatcccc atactatcat tgcttcactt
     4561 cttttttctg aagggacaaa tgacaaccta tagccaatcc tcaatgttgg tggcaaggag
     4621 aatgccctaa agtcgaaata taattctcca ccgttagata cgtaattgcg gctttgccat
     4681 gccttgtttt tatatacttt agcctggctg ttttcgtgaa aaccagtaaa ccatatgcgt
     4741 tttaggtaaa atacttttaa tatgttaata tctggataaa ctaaaggtaa gctataatca
     4801 atagcccatc ttttcatcgt tgtaaaagaa ggcgtggctg tatcccataa aatttcgtat
     4861 cctcgtgggg ctggcaaacg atctggctga aaataattgt tcaactgctg ttcttcgtat
     4921 gctactgata ttctcaagga atggtgatag tacaggcctg gcaaaaacac tttgacagag
     4981 cctacctgct gtggcgaaaa aacattggac gggcttatcg gctgtacata tatgaattca
     5041 gttttaaccc caagcatagg aaataaatct cgtgcagcac tattttttgt aaccgaagca
     5101 ttgactgaaa agactgaatt atgggcacct ttaaatatta cgccgttacg tgccgaatac
     5161 aaccggttgt catacgaata tgttgcacca ggtgccaaac taaagccata ctgcccgtta
     5221 actgtagata atggtataga tacattggta tagactatga atttgctggc acttgaacga
     5281 taatcacttg ataacccata catttttacc gaatcgccat agtctaaccc tgcctgaaaa
     5341 acagggaacc atccttgcca tgttacatga agggacgaat atagtttacc ctgccagaat
     5401 ccttgtccac cggtaaatac aaaagtgttt aacaggtttt ggtttgcaac agtatagcct
     5461 ggcattaata acctgtctaa agtggttgca gagctgttgg catacaatgg cacccatgaa
     5521 tggatatgaa acaggtgccc gattttacgg tatggacggg aatcatatcg aatatatctc
     5581 actgaatcag ggttatattt ctgaggttct tgtgcagata acttatcggc caaagccatt
     5641 tggtatgtgt caacaacctt tttctcagtc cattttactg tatcaatgac agattgcact
     5701 atttccatac cattcatagt ataattagca tatatcaatg tgtcttgact tacacaagca
     5761 tcaaaagccc cattgagggc tgatgtaacc tgatatactt ttttattatt tacattgaat
     5821 gcataaatat tgtcaattcc cgagtatgat gcactaaaat atattgtatt ttctgaccaa
     5881 aatggctttt cgacctggtg aaagccagga agaataaggg tgtcaacttt gccagattgg
     5941 atatcaacac aatataagcc ttttgcattg tcagccacca ttgtatatgc tatgctactg
     6001 ccatcggggc tccagcaagg ggattgaaca gccttttgtc caaaggtaaa tgaacgtaat
     6061 ttttctccgg tgtttgcatc caaaattacc aacgaattca tacccttcac atcattttca
     6121 accacaatca gtttttctcc atctggcgaa actgctggtg cataatatct tgacttttgt
     6181 gtaagttgta tttttttgcc agtagccatg ttgtaagcca taattactga atacgaacgg
     6241 tttgcccagc ggatatctgg cctgtattcg tcccaatata tccaatttcg ccaggcactg
     6301 attttttcag ataaaatata tcctggttca aatagctttt tctcttcacc agtattttta
     6361 tcaattgaaa caactgccgg tgtatatgaa agccctcgtc ggtaaacaac taatttgcct
     6421 tctgccacaa ccggacttgt ataattggta tatgaaaaag gcttttgagt tatcttgtcg
     6481 aatgaggtta aagacaaatg atggctttca gcggtccaaa tgctatcaag agctttatat
     6541 gtttcattat acagttgcct tctattctta gaagtcttat ttttaacagc atccagaaat
     6601 ggagcagtta tgccagggaa cgggctgtct gcaatttctt gaagcactgt ttcccaaagc
     6661 cccgtcccgt atttgattct gttgtatgct accatcaaat agccaaattc atactggtta
     6721 ggtacatagt ccttgtagct gccgtgccag gcttttcgaa aatgaaaagg ccgatgagtc
     6781 ccaataagtc tggctcttgt agccatgaca aaagaaggaa gcctgccacg cccgctattg
     6841 gtcaatgcag tttcggcagc aacagcatct ccttcgtaaa accagcttgg agcttgcaag
     6901 gctgcgttta gcccccataa gccttctcca aataaatact ttgagtatcc cagataagaa
     6961 gtattcatct ttaacatttg cccctgatga cggccttcat gaatgccaag catctcaaac
     7021 cacccaatag ctggcatatc ttgaggtgga gctgtataaa attccattcg gttgggcatt
     7081 actgtaacaa acccattaga aatagcgtta tgattgtgca ataaaatcgg ggtattgcta
     7141 atgttttgat ttatagtatt gcctgatata gacaacgagt agtccattat ggctgctact
     7201 ttctgggctt ggttttcata gcaggtgtca taaaccagcc taaagcgctt cgtctgaatt
     7261 tgcctccatt ttaccgacga tgggtcttgc cccataccgg ttaattgggc atgagcattg
     7321 caaaagaaaa taaacaaaaa actgtaaatg aacaggagtt tcaatgtttt ttgtttgaac
     7381 ttgaagttag aaaaaaaacg gcgtgttttc aaagtatatg tataatgcag acaaatatat
     7441 aaaatcaaag ctgaatcaaa aattcttaaa aaattttaac aacagacgcc taaaattttc
     7501 tattccggga atatccttat atttgcaccc taatcaaact gaatggcagt attatatcat
     7561 atacaacacc ttatcaatat tatcgtcgat tggctttatc ggccttttaa gcgatttgtc
     7621 cctaaagaaa catttaaata tgctgtttgt gggggggcta acacagcttt tgacatattt
     7681 ttatatttca ttacgtataa ttttatttta caaaaaaaaa ttattaatct taaattaatt
     7741 gctataagcc ctcatattgc ttcatttttg ttagtgttcc ccattacttt ctcaacaggg
     7801 tttttgcttt caaaatatat cacttttacc agctctacgc ttaggggcaa aacacaatta
     7861 ttacgttatg gatttactgt attggtttgt atttttctca actacatttt gttaaagctt
     7921 tttgtagaag gttttgggtg gtacccaact ccatcaaaaa tagcaaccac atgtttggta
     7981 gtaatatata gttatttttc tcaaagacat tttacattca aaacgcaaca ggctgtttaa
     8041 aagcattgcc atactgtatc agcctttagg ccttcatttg ttaaagcttc tgatacattt
     8101 aaatactggt gccttattcc tataagtgtt ttggcccagg cgtagttttt atttattatt
     8161 tctactttgt ttataatgcc ctgttcaatg ttaagcttaa aataaacatc atgttcatca
     8221 tacgaaacaa atgtataggc gggtgaatat gcataattcc attcccaggt ttgaaacttt
     8281 gattggacaa gtgaattaac tgctttatca aagtctgcct caggttcgac gatgagcccg
     8341 tcaactgacc tgacaatttt atctttcagt tcggttattg agatggaatg gtatatatct
     8401 cgaaggttag tgacagaaga acgaatagat tgaactgctt tatcaatata cacctttggc
     8461 ttaactttaa ggcaatcttt gagccgctca agattagctt ctaccagcag tgtgccgtga
     8521 tgaatagccc tgtttttata tacatgtccg gcgttgcccg aaattttgaa ttcggcgctt
     8581 cgtaaatcat ttttcccttc acgataagct ttaatgccac tctcatataa agcatccaaa
     8641 ataggctttg aaaacttctc aaaatcaatt tgttttcctt cttcagtaac agaaaaaaac
     8701 gcaaaattca ggttccccaa atcatggaaa actgtacctc cgcctgaaat acggcgaata
     8761 acaggtatat tgttttcaat gatgtagcgg tagtttattt cagcaagagt gttctgatgt
     8821 ttccccacaa caacggcgtc attgttgata taaataaaaa gaagcccttc attgctgtgg
     8881 tgtaaaagat attcttcggc agcaatgtta aaataagggt tattcgaggg actaatcaga
     8941 actttcatcc tgggccacag gttttaaatc tctatatttt gttaaagccg gaatatattt
     9001 taaaaacagg aataagaccg ttgaggctga aaaaatccca actatagccc cgcataacac
     9061 atcggttaaa taatggacac caaggtaaat gcggcttaat gatactattg ctgcccaacc
     9121 gtagatagaa aagaaaaccc atcgacgatg aaatagaaag cttactaaaa atgccaaagc
     9181 aaacgagttg gctgcatgag acgaaacaaa accgtacttc ccgcctttat aatcattaac
     9241 ataatgcaac atttcggcaa tttcggggtt atgcgatgga cgtaaccttt ctgccaatgg
     9301 ttttatcaaa cctgatgata gttggtcact taaaactata gataacccaa tggccaataa
     9361 aaaccagata ccatctttct tgtatttacg aacaataaaa tacgccaaca gcaaataaaa
     9421 cggtacccat acaaatgttt tgcttacaaa ccacataaac acatcaagcc aagggctgtt
     9481 tatcccgtta atgccaagga gtattgagat atccagttta tttattgttt ccagcatata
     9541 atatcctatt gtccattcaa tgtttcccac agcatatctt ttaactcagc gataccttta
     9601 tttgtaactg ctgaaataaa agtacattta atccctttgg gtatctggct tttcaatttt
     9661 tttatatttt cttctgtaac aaggtctatt tttgaaatag ctaaaagcct ttgtttatct
     9721 aataattcag gattgtattg ctctaattcg ttcaataaaa tgttatattc ctgggcaata
     9781 tctttcgaat ctgccgagat tacaaaaagt aaaacagaat tacgttctat atgcctcaaa
     9841 aaccttaacc ccagccctct gccttcagca gcaccttcaa taataccagg tatatcggcc
     9901 attacaaacg acttatgtcc ataatatttc acaattccaa ggtttgggac aagtgttgta
     9961 aaaggataat ctgcaatttt tggtttagct gccgaaacgc tcgacaaaag tgttgatttc
    10021 ccggcattag gaaaccctac cagcccaaca tctgccaaaa ccttgagctc aagaataaac
    10081 cagccttcaa tgcctggttc tccaggctgg gcatagcgtg gggtctgatt agtagccgat
    10141 ttgaaatgta cattgcctaa tcctccccgt cctcctttgg ccaaaatttc ttcctgtccg
    10201 tgttctatga tttcaaagag tatttcgtct gtttcggcat ttttggctac tgtccctaat
    10261 ggcacttcaa tatatttgtc ttccccaaaa gctccaaaac tctgggcact accaccattc
    10321 tggccatttt cagcatgaat atgtttggta tatttaaggt gaagtaatgt ccaaagttgg
    10381 ctgttccctc ttacaataac gtgtccgcca cggcctccat cgccaccatc aggccctcct
    10441 ttggctacaa atttttcgcg gtgcaaatgg gcgctacctg cgcctccttt tcctgagcgg
    10501 acaaatattt ttacgtaatc tacaaagttt tcgttcatat caatattccc tttccccctc
    10561 cagaggaggg gaaaaggcat ttaatttgta taatgaaatg tcatagccct gtaattttgc
    10621 cttatgggga aaggcaggct gaggcttttc ttatttttca atagctttac acagcctctc
    10681 aaatatctcg tctatgcttc ccatcccctt aataggacga aatttgccct gtttttcgta
    10741 aaactggata acaggggctg ttttagcgtt gtattcttta atacggtttt caatgatctc
    10801 gggattttga tcatcagcac gaccactggt aagcccgcgg ttcaacaagc gtttgacaag
    10861 ctcttctttg tcaacctcaa gtgaaagcat acatttcaca ggtatattgc gttctgccaa
    10921 cattacatct aaagcctctg cctgtgctgt tgtacgtggg aaaccatcga aaataaagcc
    10981 tttggcattt ttattgttgt caatttttga agctatcatg cctattacca cctggtcagg
    11041 gaccagctgg cctttatcca tgaaggcctt ggcttctatt cccagtttag tattattggc
    11101 aatttctgca cgtaaaatat cgccggtgga aagatgcact aattgatatt tttcaatcaa
    11161 tttttcactc tgagtgcctt taccgcagcc cggagggcca aataacacaa tgttgagcat
    11221 aatgttttgt ttttaatggt taaaaaattg gactatttgt tttttctttt acccagccat
    11281 tgttttacta ctgtcaaaac tggcgggatc aaggatatag caataatagc aaaaacaaca
    11341 atagtaaaat ttttctgaac aaagctgtta ttggcaaata aatagcctgc ccagcaaaat
    11401 gtgttgaccc acaagattgt tcctcctata ctgaataaaa agaaacggag gtatttcatc
    11461 tctccaatac ccgcaacaaa tggggcaaat gttcttaaga taggcataaa acgggctatt
    11521 acaacggtct tgcctccgtg tttttcataa aacttatggg ttttatcaat gtactcacgc
    11581 tttatcagcc tgaaattttt ctcatatatt ttatgtccta aaaacctgcc aatgaaatag
    11641 ttactattgt ccccggcaat ggcagcaatc attaataccg tgattagtgt aaaaatgcta
    11701 agtggtgccc ctgccccggc agctaaggcc ccggcagcaa aaagtaatga atctccaggc
    11761 aaaaatggag tgacaaccaa acctgtttcg caaaaaataa tgcaaaacaa gattaaataa
    11821 gtccaagtat catattgcga aacaatttct tttaaatgaa catcgatatg taaaatgaaa
    11881 tccaaagcaa attttagaaa ttcaatcatt tttgaaaagg tataaaaaaa ccttcaagat
    11941 tccgaaaaac ctgaaggttt atcatgatta ctattttgtc tttttaatat tttcaataaa
    12001 gtcgtcaaac agatactctg tatctaccgg cccgccggta gcttcggggt gaaactgtga
    12061 cgaaaagaaa ggttttgatt tatgccgcat cccttcgttt gtgttgtcat tgaggttgat
    12121 aaataaaggt tcccaatctt ttggcaaggt attgttgttg atagcaaaac cgtggttttg
    12181 tgaggtaata taggctttat ttgtcccaac ctgcagtact ggctgattgt ggcttcggtg
    12241 tccatacttg agtttgtatg tatcagctcc ggcagccaaa gccatcaact gattacccag
    12301 acatatcccc ataattggtt tgtccatctc aaaggcttta gaaatatttt taatggtagt
    12361 ggtacacatt ttggggtctc caggaccgtt tgagatgaaa agcccgtcaa attcttcact
    12421 aaagatgtca taatcccaag gtacccttac cagcgttaca tcacgtttga gaaggttgcg
    12481 gataatatta tattttactc cggtgtctat aagtaatact ttgtatttcc cgttgccata
    12541 tactatacgc tcttttgtgc tgaccatgtc cactaaatta actttgttag ggtcgaagta
    12601 tggtacatct ttattgtcaa caacaatttt accaagcatt gttccttttt ctctgaggcg
    12661 tttggtcaat gcacgtgtgt caatgccata tatacctggt actttatatt gtttcaacca
    12721 atcgctaagg ctcatttggg cattccagtg gttgtgttct attgaataat ctgaaatgat
    12781 taaaccagat atatgtaacc tgtctgattc atagtatttt ggaaggtttt cctccatttc
    12841 tataccaggt accccatagt tccctataag aggataagtc agaactaata tctggccttt
    12901 atacgatggg tctgtaaggc tttcgggata cccaaccatt gaagtgctga atactacctc
    12961 gccagaaaca gattcttcat aaccaaatga aaaaccttgc atttctgtac catcttccaa
    13021 aactaacttg acaggttttg tttgctccat gtaattaatt gttttattta ttatttttct
    13081 tctgtgaaag ggatatttcg tggtttatca aatcttcgca ttgctttgct ccaagtcttt
    13141 tggccaaaat ttttccttgt ttgtccaaca caaatattga cggggtactt tgaacatcgt
    13201 acagctcttt atgcttgagg gtaaatgggt taaaacagtt gatccaatcg tacatgttat
    13261 gttcattaac aaagtcaacc catttgcttt tatggtcacg attgtaaact aaacaaaccg
    13321 caatgacttc tacgttttta cttttgaggt tttgaaatac ttcatacatt ataggggttt
    13381 cttttttgca atggccgcag tcaacatccc aaaagtaaag gatagtataa tccttcatgc
    13441 gagcatgcat agaaaaagga gtgcctatgt ataaattatg tttttcaaat gtgtcttctt
    13501 tcgcaatcat aaaatgtgac ggaggaactt caaataaatc aatagccggt gacatttttc
    13561 caagcatggt aggctttttc tctgctacct gctttttcaa ctttttcaat tgggccgaat
    13621 ctgcccatgt ggcttcattg atataatatt tatctgctat ataaattgct acatcgtcat
    13681 aacccatgat ttggcttttg ttataataac taaacagagt aactaacata tacctgaaca
    13741 actccggtga agtgcgcgat tttgcaatga gcatatccac ttctttctga atagaatccg
    13801 gcaacggaa
//
LOCUS       DAOSPF010000076        13629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4531_length_13629_cov_5.543392, whole genome shotgun sequence.
ACCESSION   DAOSPF010000076 DAOSPF010000000
VERSION     DAOSPF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_13629_cov_5.543392"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK252_12355"
     CDS             <1..295
                     /locus_tag="PK252_12355"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT15544.1"
                     /translation="GIDEYIGVTIISNRTESMRLMIINKLNKGVTIYSGSRGYGKTGD
                     VNRNIEIIYTVVTRMEVSRLNAEIERIDPDAFVVMNTIKDLKGGMVRHKLFKH"
     gene            311..1627
                     /gene="rimO"
                     /locus_tag="PK252_12360"
     CDS             311..1627
                     /gene="rimO"
                     /locus_tag="PK252_12360"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT15545.1"
                     /translation="MKTRRKAKDKIHVITLGCSKNTVDSEKLMAQLNAHAVQVQHNAE
                     QSDARTVIINTCGFINDAKQESVDTILEYINAKEQGLIDNLYVMGCLSERYAKELKTE
                     MPEVDQFFGVNNIEDIVKALGYDYKSNLVGERLLSTPSHYAYLKIAEGCNRKCSFCAI
                     PLIRGKYESFAVDDLLTEAKLLAKHGVKELLLIAQDTSYYGMDIQKSSLLTTLLEKLS
                     EINEIEWIRLHYAYPAGFPKDVIDVIASNPKMCKYLDIPVQHISDNMLKIMRRGLDKK
                     KTIELIENFRSKIPGLSIRTTLLVGHPGETEADFEELYNFVCDTKFDRLGIFTYSHEE
                     DTYAARHYKDSINPGVKQERANIIMKAQQEISLLKNRQRIAHTMKVVIDNIDDAAAYG
                     RTQYDSPEVDNEVIINNPENIKVGSFYDVKIIEAGEFDLEGELVKP"
     gene            complement(1629..3521)
                     /locus_tag="PK252_12365"
     CDS             complement(1629..3521)
                     /locus_tag="PK252_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15546.1"
                     /translation="MGILSSIDFVSLFTNVISLMILIINKKYTIREIRFFLIFIISIT
                     TFYQFCIFVEWEFFTKYLDPYEDLAGALLPLTWAFLFYSIFQYFSKETIAEKEENLRI
                     TMHSISDGIIATNNQGQITFMNPTAELLTECPLSFSLGKNINKIFRVFDYDTNLPIDK
                     EIQLLIDDKKQHHSIKKCILFSNNVKQYIISAKISQIVKNENILGLIIVFSDQTEKFK
                     QEERIKANEQKLSMAISGMRAGVWDWRIEDGKLDVNQRWSEITGFFPKEIENLNIQDW
                     LKLIHPGDIAAYQAKLDEHFQGHNQYFDIKHRLINKSGEIIWVRTRGVVNEYNSKASP
                     ARMTGTIIDITENVYAETALKQKVEENELLNKQLIARNSELEDALEEVNRINDALKEA
                     KKEAEQNAFMKGNFLSTLSHEIRAPMNSIMGFTELLQTNEVTEEKKQYYLDIIKKSGN
                     YLLNVISDIIEISKLESGLVTVNIEKVSVKKAIRDVYEMFKADFEENSEVQFIIDIPE
                     DINEDFILTDGTKLQQILVNLVGNAVKFTPQGTITVSFRIESGQVKIIVKDTGIGIEP
                     QYHQAIFERFKQLDAVTKVKKKGSGLGLAICKAYLGLMKGSISLESAIGEGSTFTVTL
                     PYSIDK"
     gene            complement(3984..4547)
                     /locus_tag="PK252_12370"
     CDS             complement(3984..4547)
                     /locus_tag="PK252_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT15547.1"
                     /translation="MVIEKDKVVSVTYELKLAKGNGEIIEKVNETRPLEFLLGHGNLL
                     PKFESNLTGLKIGDSFDFILASHEAYGEIKEDAIVDVPKSVFVVDGQIDNDILTIGNM
                     IPMMDNMGNRFNGKVVSVNNDHVKMDFNHPLAGEDLHFKGEVVGIRDASAEELAHGHI
                     HQAPSCGCGSCGCDSDGCDSGGCSGCN"
     gene            4800..7064
                     /locus_tag="PK252_12375"
     CDS             4800..7064
                     /locus_tag="PK252_12375"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOT15548.1"
                     /translation="MMFDLDMIKAVYSAMGQKVGMARNKLNRPLTLTEKILYAHLDKG
                     IEIQSFRRGADYVEFNPDRVAMQDATAQMALLQFMQAGKKKVAVPSTVHCDHLIIAKD
                     GAKADLDAANVTNKEVYEFLSSISNKYGIGFWKPGAGIIHQVILENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGMPWELKFPKLIGIKLTGKMSGWTSAKDVILK
                     VAGILTVKGGTGCIVEYFGPGAETISCTGKGTICNMGAEIGATTSIFGYDKKMSDYLR
                     STGRAEVADAADSIAANLTADPEVYANPEKYFDQIIEIDLSALEPHINGPFSPDKAWP
                     LSQFAKAVKENDYPQKLEVGLIGSCTNSSYEDISRAASLASQAGDKKLKTKGEFIITP
                     GSELVRSTIERDGFLEIFAKIGGKVLANACGPCIGQWAREGAEKGERNSIITSFNRNF
                     AKRADGNPNTYAFVASPEIVTAMAIAGDLTFNPITDTLINEDGVAVKLDEPKGMELPA
                     KGFFAKDIGYQAPAEDGSKVEVKVDAKSERLQLLDSFPAWDGNDLIGLHLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFNDKTNAVKNQISGEYVPVPDAARAY
                     KKEGIGSIVVGDENYGEGSSREHAAMEPRHLGVKAVLVRSFARIHETNLKKQGIMALT
                     FAHKEDYDKIQEDDRIDIIGLKNFDPTKPLKAVLHHSNGKSEEIQLNHTYNENQFEWF
                     KAGSALNLIKYSQK"
     gene            complement(7582..>13629)
                     /locus_tag="PK252_12380"
     CDS             complement(7582..>13629)
                     /locus_tag="PK252_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15549.1"
                     /translation="SINFSRKLNTHYQDKAYIDISNDNGLTWQNIYTNFNYEQDKDWK
                     VFSYNVSEYFNKKQQTKIRFALGPIYSGKNYSGWNIDEFSVTGDSIINDVGVVKWVSP
                     LSGCGHTSHDTVKIWVKNFTTITSPSIIPVAYTFDNGATLIKDTIKQSIPAGDSILFT
                     FKTTVNLTNADLYNNVYATTLLANEDYIDNDKLTYSIAALPTYTIPVNEDFEGLKNFW
                     SAGGTNSSWLLGEPYTDSIQWAPSGYKIWKTSLYMYHNNNENSYVQSPCFNIEDTSKV
                     IVEFKYYSLQHQGTSGCALQYSIDNGTSWNVVPKHSYPWKWNWYNSNNVAVLGTAGWD
                     SITPGWVTAKQVLPNTLLNKGQLKFRFVFKSNDNAPWEGFAFDDFKVYKAPINIKLDS
                     ITSHKNNCQKVNPKKVSVHVTNNGIRSIIANKDTAILALKLNGGSEIADTLVFTSNVP
                     IGSSTTLTFNKEINAEAAGNYQLVVYHKDPYKGFYPKTDNDTVTKNFTILQNPVSGLA
                     AQYSTARLDTYKIQANINAQYTYKWQNATNSNLSSTSSLTKPPVGKCWLKLTYNATTC
                     SITDTFKVVKLIPDVGVSEIYDPVNACEFKTPVKMSVAIKNLGTDTLKKNDTIYLAYL
                     DHAKTLSKDTIKLKATFKPGDTIHYSTKKNPMNMTAFQAYNLKAYTIYKYDSVPANDT
                     TKLTIQAWGYPTVYIGPDKTVSKYDTIKLNNTFKTYLWSDNTTDTFYVARYIGKHWVR
                     VTDYHDCPASDTANLTIVAFDISANKIVSPVSACKLSSTQKFTFRYKNVGTDTIPINT
                     ILDIKYRINGNAWRTASIPLPDTLFAGDYYDYQFNQNENLSAIGQYKCEAVVKISGDI
                     DATNDTIKETINVYGYPNVNIGPDAEVEALSYTLDAGSAPNQTYLWSNGATTSNITVY
                     SSNQYSVMVTNTNTGCISRDTAIITLKIKDIQPSATDLVSIACEKAVNTATVEIINNG
                     NNPLPINTKFYVSYKLNSLAEVKDSVILTSAMPVGGKIYHTFSNLRNKFSVGANKIKI
                     YTKMTGDLQAANDTLVHNITINAVPWVDFGGTNDTILTTPPYLLIAPAGVNLQYTWQD
                     PTFFGSTYTVNNSGLYHVNVNNTVTGCSDRDTVYVKIFIPDGSITNVISPSEICLGTT
                     DTMRVEFTNVGNTIMAKGENILFTYKIGTSTPVSELYVLNSNLNPGDKLYYTFSGLKN
                     KINTAKNYSFTFYAILENDINSTNDKAYFTLKVNALPNVSLAAGQDTLQDYAGTQIFS
                     GLGSGYSYLWNTGLTNDYLTVTEPGTYTETVTNLTTGCKNKDSVYVYILKPDNAITST
                     DLPGQTCISSTLSMNVTYSNLGNMNDFEYDPLNIHVRNMTNNKIINIYRNLVADLKPG
                     HSLNIFIDRIDTILNLGANKLKIYTDSYLPDVNLNNDTLLKNIFVYPQKVFSSANILS
                     CDQANYTLPSGRVVNTSGVYLDTIASVGGCDSIMTINLKMGNSKTQTLYTTLCQGNTY
                     TSPKGKIYSETGIYRDTLVAASGCDSIIISNITVNPIKISNISKTICKGDTYNGHSQS
                     GTYTDHYLTYLGCDSTVNLTLSVKDVFADTVDVYICRGQTYKGHNTSGFYPESFVSVS
                     GCDSIVTTNLIVSDSVVVNITKEICAGQSYNGHTTAGTFRNVFASSAGCDSIVYTKLV
                     ILPAPTVNLAGGADTASVQMPYFLIAGSSDSYKYLWSDNSTDKMLIVNKGGLYKVKVT
                     DPTNNCSTTDSVFLKNKANVIFIEVTKISGLVDTCYGVTNTLSAEVKNVSGSSMTSGS
                     KIKLNLLVDNILKKEGTITLTSNLANNGTVSYNFGDISSKLHVGKNVIKVRTNIDGYE
                     DDENSYKTQTIQILSSAKPILAGGLNKVDFIGTYTLDAGTGFTSYLWSNNSTNQTLTV
                     SSSGTYWVHVTNPNGCINGDTVVMNNKVGIENGTMDRLLVYPNPVKTILKMKVPQCTE
                     NSFNLEIYSSDMRQVVTMKKMANNGFMEIDVANLKNGVYYIRIKSVECTYTEKFIKE"
BASE COUNT         3958 a         2368 c         2770 g         4533 t
ORIGIN      
        1 tggtattgat gaatatatcg gggttactat catttctaat cgcactgaat caatgaggct
       61 gatgattatt aacaagctca acaaaggggt caccatttac tctggttcaa gagggtatgg
      121 caaaactggc gatgtaaacc gaaatattga aataatatat actgttgtga cccgtatgga
      181 agtttcgcgt ttaaacgccg aaattgaacg tattgaccct gatgcttttg ttgtaatgaa
      241 tacaattaaa gacctcaaag ggggaatggt tcgtcataaa ctttttaaac attagaattt
      301 cttacattac gtgaagacaa gacgaaaagc caaagataaa atccatgtga taacgcttgg
      361 atgttctaaa aatactgttg attctgaaaa gttgatggca caactcaatg cacatgcagt
      421 tcaagtgcag cacaacgctg aacagtctga tgccagaacg gtaatcatca atacctgtgg
      481 ctttattaat gatgccaagc aggagtctgt tgataccatt ttggaatata tcaatgccaa
      541 ggagcaaggg cttattgaca atctttatgt gatgggttgc ttgtctgaaa ggtatgccaa
      601 agaactaaag actgaaatgc cagaagtgga ccagtttttt ggggtaaata atattgaaga
      661 tattgtaaaa gcgttgggtt acgattataa atcaaattta gtcggcgaac gcttgctttc
      721 aacgccttcg cattatgcat atctcaaaat tgctgaaggc tgcaaccgta aatgttcttt
      781 ttgtgccatt ccattgattc gtggaaagta cgagtcgttt gctgttgatg atttgctcac
      841 tgaagctaaa ttacttgcta aacatggagt gaaagagctg ttgctgatag cccaggatac
      901 ctcgtattat ggaatggata tacagaaaag ctctctgcta acaaccttgc ttgaaaagct
      961 ttcagagatt aatgaaattg aatggatacg tttgcattat gcttatccgg ccggatttcc
     1021 taaagatgtc attgatgtaa tagcttctaa ccctaagatg tgcaaatatc ttgatatccc
     1081 tgtccagcat atttcggaca atatgctgaa aatcatgcgt cgaggactgg ataagaaaaa
     1141 gactatagag ctcattgaaa atttcagaag caaaataccc ggtttgtcta tacgtacaac
     1201 cttgctggtt gggcatccgg gcgaaacaga agcagatttt gaggagttgt acaattttgt
     1261 ttgtgatact aagtttgaca gattaggcat atttacttat tcacacgaag aagatactta
     1321 tgctgcccgc cattataaag acagtataaa ccccggcgtt aaacaggaac gcgccaatat
     1381 tatcatgaaa gcacaacagg aaatatcgtt gctcaaaaac aggcagcgta tagctcatac
     1441 catgaaagtg gttattgata atatagacga tgcagctgct tatggtcgta cacaatacga
     1501 ctcacctgaa gttgataatg aagttattat caataaccct gaaaatatca aggttggttc
     1561 attttatgat gtcaaaatta ttgaagcagg agagtttgac cttgaaggtg agttggtgaa
     1621 gccctaattt atttgtcaat agagtaaggc agagtaactg taaacgtaga accttcgcca
     1681 atggcacttt caagagaaat actgcctttc atcaatccga gatatgcctt acaaatagca
     1741 agccccaagc ccgaaccttt tttcttaact ttagtgactg catctaactg tttgaaacgc
     1801 tcaaaaatgg cttggtgata ttgtggctca atgcctatac cagtgtcttt tacaataatc
     1861 tttacttgcc cgctttcaat gcgaaaacta accgtaattg tcccttgggg ggtaaatttt
     1921 acagcattgc caaccaggtt gactaatatc tgttgcagtt tagtgccatc tgtaagtata
     1981 aaatcttcat ttatatcttc tggaatatca ataataaatt gtacttcgct gttttcttca
     2041 aaatcggctt taaacatttc atacacatcc ctgatggcct tttttacaga aactttttca
     2101 atgttaacag taaccaatcc tgattcaagc ttcgaaattt caatgatgtc gctgattaca
     2161 ttaagaaggt aattgccact ttttttaatg atgtccaggt aatattgctt tttttcttct
     2221 gttacttcgt ttgtttggag cagctctgta aagcccatga ttgaattcat cggggcacgg
     2281 atttcgtgag acagagtaga taaaaaattg cctttcatga acgcattttg ttctgcttct
     2341 tttttggctt cctttagcgc atcgtttata cggttaactt cttccaaagc atcttcaagt
     2401 tctgaatttc tggcgattaa ctgtttgtta aggagttcgt tttcttcgac cttttgtttt
     2461 agggctgttt cagcgtaaac attttctgta atatctataa ttgttccggt cattcttgca
     2521 gggctggctt tggagttata ttcgtttaca acgcctcttg ttctgaccca aatgatttct
     2581 ccagatttgt ttatcaaccg gtgcttaata tcaaagtatt ggttgtggcc ttgaaaatgc
     2641 tcatcgagtt ttgcctgata agcagcaatg tctccaggat gaatcagttt gagccagtct
     2701 tgaatattca gattttctat ttctttaggg aagaagcctg taatctcaga ccagcgctgg
     2761 tttacgtcaa gttttccatc ttcaattctc caatcccaga caccggcacg catgccggat
     2821 atagccatcg aaagcttttg ttcgttggct ttaatccttt cttcttgttt gaatttttca
     2881 gtttggtcgc taaaaacaat gataagtccc agaatatttt catttttaac aatttgcgaa
     2941 atcttggccg aaatgatgta ttgcttcaca ttatttgaga aaagaatgca tttttttatt
     3001 gaatggtgct gttttttatc atcaataagc agttgtattt ctttgtcgat aggtaagttt
     3061 gtgtcataat caaaaaccct aaaaatttta ttgatatttt tccctaaaga aaaacttaaa
     3121 ggacattcgg ttaataattc tgctgtaggg ttcataaaag tgatttgccc ttggttgttg
     3181 gttgcgataa tgccatcgct gatagaatgc atggtgatac gcaggttttc ttctttctcg
     3241 gctatagtct ctttgctgaa atattgaaat atcgaataga ataaaaatgc ccaggttagc
     3301 ggtaacaagg ctccggctaa atcttcataa gggtcaaggt atttggtaaa aaactcccat
     3361 tcaacaaaga tgcagaactg ataaaaggtt gttattgaaa tgatgaatat gaggaaaaag
     3421 cgaatttctc taattgtata tttcttgttg attatcaata tcatcaggct aatgacattg
     3481 gtaaatagtg aaacaaaatc gatagaagaa agtatgccca cagcttaatt ttttatttct
     3541 tttttgttta ttagcaaaat ccaaataagt aatagcatac tactaatggc ataagatatg
     3601 tgttcaatca tattgtaaac acttgggaaa aaataactct cgaggttagt acatatactt
     3661 gccattaata aagataagta tgcgccaagt aacaattgcc aatgctttat ttttattatt
     3721 gatttaaaat ataaaacaat aaaaatcaat acgcataggg ataatagaag catcacaact
     3781 tcattctctt tcattttttt gatatttgaa aataataata aagtatttat ttaaggttca
     3841 tggggtttga tgttttaaag atacacttaa ttttttttat aatttaatac aaatgtattt
     3901 ttttgttgaa gtagtaaata aacagtaata taaaaaaagc cacaattgtt aatttgtggc
     3961 ttctaaaata tttaaaacaa agattagttg catccactac acccgccaga gtcgcatccg
     4021 tctgaatcgc aaccacaact gccacatccg catgaaggag cctggtgaat atgcccatgg
     4081 gcaagttctt cagcagaagc atccctgatg ccaacaactt ctcctttgaa atgaaggtct
     4141 tcacctgcca aaggatggtt gaaatccatc tttacatgat cattattaac gctaacaacc
     4201 tttccgttga aacgattgcc catgttatcc atcattggta tcatgttgcc aatggtgagg
     4261 atgtcgttgt ctatttgtcc atctacaaca aatacagact taggaacatc aacaatagca
     4321 tcttctttta tttcgccata agcttcatgg ctggccaaaa taaaatcaaa gctatcgcct
     4381 atttttagcc ctgtcaggtt ggattcaaat ttggggagaa gatttccgtg acctaataaa
     4441 aattcaagcg gacgggtttc atttactttc tcaataattt cgccatttcc tttggcaagt
     4501 ttcagttcat atgttaccga aacaactttg tctttttcaa ttaccatttt ttaatcaatt
     4561 aaatatctgg caaagtaacg tcaaatattc ttatttgttt aaatacaata tcaaaattta
     4621 ctaattttta atataataat ttagggtgtt gaaaatattg aacaattatt aattatgtta
     4681 tgttaaaatg ctttaaagtt gcatagtttg tgtgactcaa ataaaaattt taagctttgc
     4741 cagaataagg ctaaatttgc aaggtgcata tattgtaaaa cagtttaacc gttaaaaaca
     4801 tgatgtttga cttggacatg attaaagccg tgtattcggc aatgggacaa aaagtcggga
     4861 tggctcgcaa taaattaaac aggcctttga cattgaccga aaaaatatta tatgcccatc
     4921 ttgataaagg tattgaaata caaagtttta ggcgtggcgc tgattatgtt gagtttaacc
     4981 ccgaccgtgt ggccatgcag gacgctacgg ctcaaatggc cttgttgcag tttatgcagg
     5041 ctggtaaaaa gaaagtagct gtaccgtcca ctgttcattg cgaccacctt atcattgcca
     5101 aagatggagc aaaggcagac cttgatgctg ctaatgtgac taacaaagaa gtatatgagt
     5161 tcctttcgtc catttcaaat aaatatggta taggcttttg gaagccaggc gctgggatta
     5221 ttcaccaggt aatcctcgaa aattatgcat ttcccggggg aatgatgatt ggtactgact
     5281 cgcacaccgt aaatgccgga ggtttgggta tggtagcaat tggtgttggt ggcgcagatg
     5341 ctgtagatgt gatggctggt atgccttggg agcttaaatt tcctaagctt attggcatta
     5401 aattgacggg gaagatgtct ggatggacat ctgctaaaga tgtaattttg aaagttgcag
     5461 gtatattgac tgttaaaggt ggtactggtt gtattgtcga gtattttgga ccgggagccg
     5521 aaactatttc gtgcaccgga aaaggtacta tctgtaacat gggggctgaa attggtgcta
     5581 ccaccagtat ttttggttat gataaaaaaa tgtcagatta tttgcgttca acaggtcggg
     5641 cagaagtggc ggatgctgct gacagtattg ccgctaatct tactgcagat cctgaagtat
     5701 atgccaatcc tgaaaaatat tttgaccaaa ttatagaaat agacctttct gcactagaac
     5761 cgcatatcaa tggcccattt tcgcctgata aggcatggcc tttgtctcag tttgccaaag
     5821 ctgtaaaaga aaacgactat cctcaaaaat tggaagttgg attaataggc tcatgtacaa
     5881 actcatctta tgaagatatt agccgtgcag catctttagc ttctcaggca ggtgataaaa
     5941 agttgaaaac taaaggtgaa tttattatta ctcctggttc tgagcttgtc agatcaacaa
     6001 tagagcgtga cggttttctt gaaatatttg ctaaaattgg aggaaaggta cttgctaatg
     6061 cctgcgggcc atgtattggg caatgggcca gagagggggc cgaaaaaggt gaaagaaact
     6121 ctattatcac ctctttcaac agaaattttg ccaaacgtgc cgatggtaac cccaatactt
     6181 atgcttttgt tgcgtcgcct gagattgtta cagcaatggc tatagccgga gaccttactt
     6241 ttaaccctat tactgatacc ctgataaatg aagacggcgt tgctgttaag cttgacgagc
     6301 ctaaaggcat ggaattgcct gctaaaggtt tctttgcaaa ggatattggc tatcaggcac
     6361 cagctgaaga tggcagcaag gtagaagtta aggtggacgc taaatctgaa cgcttgcagc
     6421 tgctcgattc ttttcctgcc tgggatggaa atgatttgat tggtttgcat ttgcttatta
     6481 aggccaaagg taaatgtact actgaccata tctctatggc aggtccatgg ctcaaatacc
     6541 gtggccacct cgataatatc tcaaacaatc tgttgattgg ggctgtaaat gcttttaatg
     6601 ataaaacaaa tgctgtaaaa aatcaaatca gcggagagta tgttcctgtt ccggatgctg
     6661 caagggctta taaaaaggaa ggaataggca gtattgttgt tggggatgaa aattatggag
     6721 aaggctcttc tcgcgagcat gctgctatgg aacctcgaca tttgggggta aaggctgtat
     6781 tggtgcgttc ttttgcccgt attcatgaga ctaatctcaa aaagcagggc ataatggcct
     6841 tgacctttgc ccataaagag gattatgaca aaattcaaga ggatgacagg attgatatca
     6901 ttggcctgaa aaactttgat ccaactaagc ctttgaaagc agttttgcac catagcaatg
     6961 gaaaaagcga ggaaatacag ctgaaccata cgtataatga aaatcagttt gaatggttca
     7021 aagcaggttc tgcattaaac ttgataaaat atagtcaaaa ataaaagtgt cgatttaggt
     7081 ttttaaaaat tcaaggcttg ccgtaaattg gctaaaaaaa gctttatcga cggttctggg
     7141 caacaactcg cttcactcaa acagttgccc tcgcttcgtc gataaagctt ttttcttaac
     7201 gtcatttccg tcaggccatt agcagagacg tttctatgaa aaaggcgtag cattaaaaag
     7261 aaaaaattcc ttttcatcgc ttttttgttc gatttcggcc gaggagccta acattcattt
     7321 ggttgcatat tgcaacatta aaaggcttcg agggtgaaag agttaaggcg ataagatttt
     7381 ttgagcaact tttttcggta aaaagttgta gagacttagt atttattgct attcaatact
     7441 tttttaacaa taattgacaa tgttattttt tcattgaact gacattgaat aatatttaaa
     7501 actgcagagg gccgcaaagt ttggaatcta ctttgcggcc ctctgcggtt aacattaagg
     7561 ccttcgctgt aaaagatagt gttattcttt aataaacttc tccgtataag tacattcaac
     7621 agatttaatc ctgatgtaat aaacaccatt tttgaggttt gcaacatcta tctccataaa
     7681 tccattattg gccatctttt tcatggttac aacttgtcgc atatccgaag aataaatttc
     7741 caaattaaaa ctgttttcag tacattgagg tactttcatt tttaaaatgg tttttacagg
     7801 gttaggatag accaatagtc tgtccattgt cccattttca ataccaacct tattgttcat
     7861 aactacagta tcgccgttta tacatccatt ggggttggtt acatgtaccc aataggtgcc
     7921 gcttgaagat actgttaaag tttggttagt actgttattg ctccataaat atgaagtaaa
     7981 accagttccg gcatcaagag tatatgtacc tataaaatct actttattta aacctcctgc
     8041 taagataggt ttagcagagc ttaatatttg gatagtttgg gttttatagc tattttcgtc
     8101 gtcttcatag ccatcaatat tggttctaac ctttattaca tttttaccaa catgtagttt
     8161 gctgcttata tcaccaaagt tatatgaaac agttccatta ttggcaaggt ttgaggtaag
     8221 tgtaattgtt ccttcttttt tcagaatatt gtcaaccagt aaattaagtt ttattttact
     8281 gcctgaagtc atactgctgc cgctaacatt ttttacttcg gccgaaaggg tatttgttac
     8341 tccatagcag gtatcaacaa gtccactgat tttggtaact tcaatgaaaa taacattagc
     8401 cttgttcttc aagaaaacgc tgtctgttgt tgagcagtta tttgtcgggt cggtaacttt
     8461 aactttatat aatcctcctt tattaacaat aagcattttg tcagtggaat tgtcgctcca
     8521 taaatattta tagctgtctg agcttcctgc tatcaaaaaa tatggcattt ggacagaagc
     8581 tgtgtctgca ccgccagcca ggttgacagt tggagcaggt aaaattacaa gttttgtgta
     8641 aacaatacta tcgcatcctg cagaagaggc aaatacattg cggaatgtgc ctgctgtggt
     8701 atgcccgttg tacgattgtc cagcgcagat ttcttttgta atattaacaa ctacagagtc
     8761 tgaaactata aggttagtag taacaatact gtcgcaacct gagacagata caaatgattc
     8821 gggataaaag cctgatgtat tgtgaccttt ataagtctgg cccctgcaaa tatatacatc
     8881 tacagtatct gcaaatacat cttttacaga aagggtcagg ttaacagtac tgtcacatcc
     8941 caagtacgtt aagtaatggt ctgtatatgt accactttga ctatgcccat tgtatgtatc
     9001 ccctttgcat attgttttgg atatattact tatttttatt gggtttacag taatattact
     9061 gattataata ctgtcgcatc cggatgctgc aaccagtgta tctctgtaaa taccggtttc
     9121 actatatatt tttcctttag ggcttgtgta tgtattgcct tggcatagtg ttgtatataa
     9181 tgtttgtgtt ttgctgttgc ccatttttaa gtttatggtc atgatactgt cacatcctcc
     9241 aacagaggca atggtgtcaa ggtaaactcc cgaggtgttt acaacacggc cggaaggtaa
     9301 agtgtaattt gcctggtcgc acgatagtat attagccgat gaaaaaactt tttgaggata
     9361 tacaaatatg ttcttcaata acgtatcatt attcaggttt acatccggga gatacgagtc
     9421 ggtgtatatt ttgagtttgt ttgccccaag gttgagtatg gtatcaatcc ggtcaataaa
     9481 aatgttcagg ctgtggcctg gtttaagatc tgcaaccaaa tttctataaa tgttaatgat
     9541 tttgttattg gtcatgtttc taacatgaat attcaacggg tcgtattcaa aatcattcat
     9601 attgcccagg tttgaataag tgacattcat tgaaagggtg cttgatatgc aagtctgtcc
     9661 tggcaggtca gtactggtta tggcattgtc cggtttcagg atatacacat aaacgctgtc
     9721 tttgtttttg caacctgtgg taaggttggt tacagtttca gtataagtgc cgggttctgt
     9781 tactgtaagg tagtcatttg tcaaacctgt attccaaagg taagaatatc cactgcctaa
     9841 gccagaaaat atttgtgtac cggcataatc ttgaagggta tcttgccctg cagctaaaga
     9901 tacattgggc aatgcattta ccttgagcgt gaaataagct ttgtcattgg tactattgat
     9961 gtcattttca agtatggcat aaaaagtaaa gctatagttt ttggcggtat tgattttgtt
    10021 tttcaggcca ctaaaggtat agtaaagttt atctcccgga ttgaggtttg aattcaatac
    10081 atataattca gaaacgggtg ttgaagtacc aattttataa gtaaacaaga tgttttcacc
    10141 tttagccatg atggtatttc ctacattggt aaattcaacc ctcatggtat ctgttgttcc
    10201 aaggcatatt tctgaaggtg agataacgtt tgtaatactc ccatcgggga taaatatttt
    10261 gacatacacc gtatcccggt cagagcagcc tgtaacagta ttgttaacat ttacatggta
    10321 cagtccgctg ttattaacag tataagtgct gccaaaaaaa gtcgggtctt gccaggtgta
    10381 ttgcaagtta actccggctg gggctataag taagtatggc ggagtggtca aaatggtgtc
    10441 attggtccct ccaaaatcga cccacggaac agcattgatg gtgatattat gtacaagggt
    10501 gtcattggca gcttgcaaat ctccggtcat ttttgtgtat atttttattt tattagctcc
    10561 cacgctgaat ttgtttctga gattgctaaa cgtatggtat attttacccc caacaggcat
    10621 tgctgaggtt aatataacgg agtcttttac ttctgctaag gagtttagtt tatatgaaac
    10681 gtaaaactta gtatttatag ggagtgggtt gttcccatta ttaataatct caacagttgc
    10741 cgtattgaca gctttttcac aggctatact taccaagtcg gttgctgagg gctgaatatc
    10801 ttttattttt agtgtaatta tggcagtgtc acggcttatg cagcccgtgt tagtgttagt
    10861 aaccattacg ctgtattggt ttgacgaata aacagtaatg ttacttgtag ttgctccatt
    10921 actccataaa taggtttggt taggggcgct cccggcatct aaggtataac ttaaagcttc
    10981 tacttcagcg tcagggccaa tgtttacatt ggggtatcca tatacgttta tcgtttcttt
    11041 tatggtgtca tttgtggcat ctatgtcgcc ggaaattttt acaacagctt cgcatttgta
    11101 ttgacctata gccgataaat tttcattttg gttaaattga tagtcataat aatctccggc
    11161 aaaaagagta tcaggtaaag gaatagaagc tgttctccat gcattgccat ttattctgta
    11221 tttgatatct aagatagtat ttataggaat ggtatccgta cctacatttt tgtaacggaa
    11281 agtaaatttt tgtgtacttg aaagtttaca tgccgatacg gggcttacaa ttttattggc
    11341 gctaatgtca aaagctacaa tggtcagatt agctgtgtct gatgccgggc aatcatggta
    11401 atctgttact cttacccaat gcttgccaat atagcgggct acataaaatg tgtcggttgt
    11461 attgtcgctc cagaggtatg ttttaaaagt gttgttcagc ttaatcgtat catatttcga
    11521 gactgttttg tctggcccga tataaaccgt tgggtatccc caagcctgaa tggtcagttt
    11581 ggtggtgtcg ttggctggta ctgaatcata tttataaata gtgtaggctt tgaggttgta
    11641 tgcctggaag gcagtcatat tcatcgggtt ctttttggtc gaataatgaa ttgtatcacc
    11701 gggtttgaat gtagccttca gtttgatggt gtcttttgaa agtgttttgg catggtctaa
    11761 ataagccaaa tatatggtat catttttctt taacgtatct gtacctaagt ttttgatggc
    11821 aacagacatt tttacagggg ttttaaattc gcaggcattt acagggtcat atatttcgct
    11881 tacaccaaca tcaggtatca gctttactac tttgaaggta tctgttatac tgcatgtagt
    11941 tgcattgtag gttagcttta accagcactt acctacagga ggctttgtta gcgatgaagt
    12001 tgaagataaa tttgaattgg tggcattttg ccatttgtag gtatattggg cattaatgtt
    12061 ggcctgaatt ttataggtgt caagacgggc agttgagtat tgcgcagcta accccgatac
    12121 agggttttgg agtatagtaa agttttttgt aacggtgtcg ttgtctgttt taggataaaa
    12181 acctttgtat gggtctttat gatatactac caactgataa tttcctgcag cttcagcatt
    12241 tatttcttta ttgaaagtga gcgttgttga acttccaata ggaacatttg atgtaaatac
    12301 taaagtgtct gctatttctg agccgccatt gagctttaaa gccagtattg ctgtgtcttt
    12361 gttggctata atgctacgga tgccattgtt ggtaacgtgt acgctcactt tcttagggtt
    12421 taccttttgg cagttgtttt tgtgcgaggt gatagagtct aattttatgt ttatcggggc
    12481 tttatacacc ttgaaatcat caaaagcaaa tccttcccat ggggcattat cattactttt
    12541 gaatacaaac ctgaatttta gctgtccttt atttagtaaa gtattgggca atacctgttt
    12601 tgcggtaacc catccgggag taatactatc ccatcctgca gtgccaagta cagcaacgtt
    12661 attgctatta taccagttcc atttccatgg gtacgaatgt ttgggtacaa cgttccagct
    12721 ggttccgtta tctatgctat attgtaaagc acagcccgag gtcccctgat gttggaggct
    12781 gtaatatttg aattcaacaa ttacttttga agtgtcttct atgttgaaac aagggctttg
    12841 aacgtatgaa ttttcgttat tgttgtgata catgtataat gaagttttcc agatcttata
    12901 tcctgaaggc gcccattgga tgctatctgt ataaggctcg ccaagcagcc atgaagagtt
    12961 tgttcctcct gcggaccaga agtttttcaa accttcaaaa tcttcattta cgggaatagt
    13021 ataggtaggt aaagccgcaa tgctatatgt taacttgtcg ttgtcaatgt aatcttcgtt
    13081 agccagcagt gtagtggcat atacattatt atataagtct gcattggtaa ggttaactgt
    13141 ggttttgaat gtaaaaagga tactatcccc cgcagggatg ctttgtttga tggtgtcttt
    13201 tatcagagtt gccccattgt cgaaagtgta agctacaggg ataatggagg gagaggtaat
    13261 cgttgtaaag tttttgaccc aaatttttac agtatcatgg cttgtatgcc cgcagcctga
    13321 aaggggagat acccatttta ctacacccac atcattaata atagagtctc cggtgacaga
    13381 aaattcatca atgttccatc cggaataatt ctttccgctg tatataggcc ccaaggcaaa
    13441 cctgattttg gtttgttgtt ttttgttgaa atattcagaa acattatatg aaaatacttt
    13501 ccaatctttg tcttgttcat aattaaagtt tgtatagatg ttttgccagg taaggccatt
    13561 gtcgttgctt atatctatat aagctttatc ctgatagtga gtgtttagtt ttcggctaaa
    13621 gtttataga
//
LOCUS       DAOSPF010000077        13449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4607_length_13449_cov_7.177766, whole genome shotgun sequence.
ACCESSION   DAOSPF010000077 DAOSPF010000000
VERSION     DAOSPF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_13449_cov_7.177766"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK252_12385"
     CDS             <1..950
                     /locus_tag="PK252_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT15550.1"
                     /translation="NTIHGEGCKTVTGYSQEEFISDQYLWFNIIHEEDRDAVINQAKI
                     LMQNIEPSSIEHRIIHKSGIIKWVRNTPVIRRNEQGEMIGYDGLIADITDRKMAEIAL
                     KETERKILNATIEAEEKERAHIARELHDGIGPLLSTIKLYIEWLNKPELQTPKEEIVN
                     NANKTVAETISAIKEISQKLSPYIVTNFGLEHGLKEYIDKLKESCNIVINYESNLKIR
                     LSSEIEVTLYRIILECINNTIKYAEAKKIYIRLNVFEQQLFVDYEDDGKGFDYSTLKS
                     DGNGQGLINIKNRIASMGGKITIESRPGHGFKMKVITNI"
     gene            1014..1424
                     /locus_tag="PK252_12390"
     CDS             1014..1424
                     /locus_tag="PK252_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15551.1"
                     /translation="MKGKRLKILIVDDSSSFRSAAAMFLTNELNCEIVGEAATGREFL
                     KMIKNLDVDIVLMDIQMPDMDGISATKEWCLRYPHTKVLAITMFTDKAFLLQLIEAGF
                     KGCVFKASFFSEILTAIEAIMDGKLYFEKDMPVS"
     gene            1498..2187
                     /gene="cmk"
                     /locus_tag="PK252_12395"
     CDS             1498..2187
                     /gene="cmk"
                     /locus_tag="PK252_12395"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT15552.1"
                     /translation="MNNTPNYVIAIDGYSSCGKSTFAKAIAALLQYIYIDTGAMYRAV
                     TLYALNNKLITNGVANKEAVIEHLPKINVRFERETDGQVKTILNGEDVSETIRNLEVS
                     KNVSAISAIKEVRQKMVELQRSMGKQKRVVLDGRDIGTVVFPDAEIKIFMTADPKVRA
                     QRRYDELIAKNMPASFDEVYQNIIDRDHLDTTRAESPLTKADDAIVLDNTLLTPQEQV
                     DWFLELVKSKG"
     gene            2211..2618
                     /locus_tag="PK252_12400"
     CDS             2211..2618
                     /locus_tag="PK252_12400"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOT15553.1"
                     /translation="MAHIVWDEKMSVQVGLMDTQHKKLIEVINDFYSSMGSNNSKEKT
                     LEVIKAMKDYTFFHFNAEEKLMQQNAYPDFEKHKKEHQAFIDKVHDMEERYKSGKLIL
                     TLEVASFLKTWLTNHIMQVDKKYGDYLNARGIR"
     gene            2636..3508
                     /locus_tag="PK252_12405"
     CDS             2636..3508
                     /locus_tag="PK252_12405"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT15554.1"
                     /translation="MKVTIDEKSGFCNGVVIAISHVEEELKKSGHLYCLGQIVHNNVE
                     VERLEKMGLETINYEQFKQLKDCKVLIRAHGEPPLTYQIAKQNNIELIDASCKVVLKL
                     QERIRNEYNNTVEKAQIVIYGKKGHAEVNGLIGQTGGDAIVVSSDADLENIDYTKPAH
                     LYSQTTMSIEGFQQLADKIRQRMQQAQGKETVALYIHDTICRQVSNRAPHLRQFSKDN
                     DVIIFVSGRDSSNGKVLYEECRQVNANTHFVSDIADIQSNWFVNKQTVGICGATSTPM
                     WLMEKVATFIAQIK"
     gene            3521..5971
                     /gene="priA"
                     /locus_tag="PK252_12410"
     CDS             3521..5971
                     /gene="priA"
                     /locus_tag="PK252_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT15555.1"
                     /translation="MLYADIILPFPLQQYFTYTVPVELCTQVVMGVRVIVPFGKSKFY
                     TGIVASLYENQAVNSQIELKSIVSVVDNKPVISEYQLRFWQWMASYYMCSPGEVMRAA
                     LPAGLKLESESFVVANGQFDGIDELSEQESTVYGFIQSKKRITLEDVYKSVKIPRVVS
                     VVKKLYEQKAIFIDEKIVEKYQPKQQTYVRPCNTDATFDETMKMLAKWPKQQQLYERL
                     LQLIDTTNADVEIGVSQKLLLDSEGFSSSVVKTLEKKGLLSVYSREMSRFKESTNENE
                     TLVELSAAQNIAKSAIEKYFEDKNIVLLHGITSSGKTEIYFHLIKDVLEKGKQVLFLL
                     PEIAITSQMINRMQRYFKNKVGVFHSKYSDSERVEVWKRLMESNAEGIQIILGVRSSV
                     FLPFQNLGLVIVDEEHENTYKQNDPAPRYHARDAAMVLAQMYNAKVLLGSATPSVETY
                     FHATSGKYGLVNLNERFSGLELPKVELVNLRIAKKKGNTTSYFSNRLLQAIDETIADQ
                     RQVILFQNRRGFSPYLQCACSWIPRCRHCDVTLTYYKNQNKLVCHYCNFVANVPDKCI
                     ECGGTDIKTMGLGTEKVEDELKLMFPQYRVARMDLDSIRGKFAHEHIIADFENGNVDI
                     LVGTQMVTKGLDFQHVGLVGILNADSLLNYPDFRAYERSFQLMQQVSGRAGRKGKQGL
                     VVIQTYSPANPEIRFVLKNDYQAHYQFQITERKEFLYPPYTRIIHLMLKHVDEKLTWD
                     AANVLAKLLKQKLGNRVAGPARPLVGRIQNQYIVGIVIKLERTLDLAKIKLEISHIIA
                     VSMANFKAVRVTIDVDPM"
     gene            6039..7034
                     /locus_tag="PK252_12415"
     CDS             6039..7034
                     /locus_tag="PK252_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOT15556.1"
                     /translation="MVSDNLKTFFKDLTSKRILIIGDVMIDAYMWGKVDRISPEAPVP
                     VVTCTKQDNRLGGAANVAINIAAMGANPILCGIIGNDDKGTLFLKLMQDNHLSNAGIC
                     IVKGRKTTIKTRIIGGNQHLLRIDDETTQPLEGAEIVNFSQHLFDLINELKVDAIIFQ
                     DYDKGLLTPALINEVIHFANKKDIPVLVDPKKRNFHAYHGATLFKPNFKEFCEGLKVE
                     LSKENLQDVYTVAKDFLNSHNIKILLLTMSEKGVMICTTENFHHIPAHVRDIADVSGA
                     GDTVISVATLGIACNLDLKEVAEVSNLSGGLVCEKVGVVPVDKAQLEREVRKYLH"
     gene            7130..7202
                     /locus_tag="PK252_12420"
     tRNA            7130..7202
                     /locus_tag="PK252_12420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7163..7165,aa:Pro,seq:cgg)
     gene            complement(7209..8099)
                     /locus_tag="PK252_12425"
     CDS             complement(7209..8099)
                     /locus_tag="PK252_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOT15557.1"
                     /translation="MKQVLLYNRNTQHIESEKTYQADGLAFLYKTKIGKTLIHSILNK
                     KMVSKIYGQAVKSRQSRYLIKKFIHHYNIDTSEIKQPIESFRNFNAFFIRELVPGARP
                     VDETPSHLISPADSRLMVFDLANTGALPVKGYWYSLLDLVKDKKIAQEYHDGWCFVYR
                     LAPSDYHRFCYIDNGTQTGVKRLRGVLHSVNPIALSATKNLLSKNYRELTVLETENFE
                     KVVQLEVGALLVGKIVQRKRHAHTFRRGEEKGWFEYGGSTIIQLFKKQYITPDPDILE
                     HSVKGIETLVKMGEKTGAKL"
     gene            8490..9005
                     /locus_tag="PK252_12430"
     CDS             8490..9005
                     /locus_tag="PK252_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOT15558.1"
                     /translation="MENNNGSLLKATMNYGLITGLIVIVYSLLLYIIGLHLNQYFGYL
                     TIVIMAACIFIFTKQYRDKQNEGFISFGKAFQLGILIGVFAAILLSFFSYIEVTFIDP
                     TIVEKQLDIQREKMLAKGISEEQVEMGIQMAKKMMTPAMLFIVSILSFTFWAAIISLI
                     TSAILKKNSSN"
     gene            9042..9992
                     /locus_tag="PK252_12435"
     CDS             9042..9992
                     /locus_tag="PK252_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT15559.1"
                     /translation="MDLSIIVPVYNEEESIPELVAWIDRVALANHLSYEVVMIDDGST
                     DNSWELIESVAQANKNIRAIKFRRNYGKSPALQCGFQAAKGDVVITMDADLQDSPDEI
                     PDLYKMITRDGYDMVSGWKQKRYDPLAKRLPSKIYNAVARMVTGIKLHDFNCGLKAYK
                     SRVVKSIEVYGDMHRNIPYLAYQAGFKKIGEKVVQHQKRKYGYSKYGWQRFVTGFLDL
                     MSLVFVTKFGKRPMHLFGVLGSLMFIAGLSWAAFLGIDKLYAVSHNLPAKLLTQSPYF
                     YLALAAMIIGTQLFLAGFLAELIVRSSADRNTYLIDKSID"
     gene            9992..11125
                     /locus_tag="PK252_12440"
     CDS             9992..11125
                     /locus_tag="PK252_12440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15560.1"
                     /translation="MKALLLGPAYPLRGGIANFNESLAVAFIKNSIDTHIVSFYFQYP
                     SFLFPGKTQFTKNKPAHFIEIKSILSSINPISWYKTARYIEQENPDVIIVQYWMPFMA
                     PALGSVLRLIRRKRAPKVIAIVHNAKPHEKQPGATMLTKYFLNVCHGYICLSKSVLKD
                     LDEFTVNPNKIFTPHPVYDIFGDKVPKNEARKYLSIGLQERVICFFGIIRKYKGLELL
                     LNAMANPSVRDLNLKLIVAGEFYDNKQFYYDLVKTLNIESNVVFTDRYIPTSEVKYYF
                     CASDMIVQPYSDATQSGVTQIAYNFERPMLVTNVGGLAEIVFHNHTGYVTEVNAESIA
                     LSISDFYLNHREEEMSANVAMEQYRFSWHNMVDAILNLTENLQ"
     gene            complement(11133..11852)
                     /locus_tag="PK252_12445"
     CDS             complement(11133..11852)
                     /locus_tag="PK252_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT15561.1"
                     /translation="MKKYLTLLIAVFTITLSAQDFEFQLSSFYYFLPNQSQYYSNPID
                     RNITKQQLEYKEKPGIALSAFYKLTLINKNDFSIGLGVNWHSYNLKYFERNVEQGDIN
                     PLITNNSFVIETGAINPTCAANLVWLNIPAVYHYEIIDNLNIDGGVQLSALLYNNQEY
                     KYYIQKNNTLAINNKDYQKGLSKYMASLVFGGEYRIIKELSVLLHYHFSLTSLYEKEY
                     QYIDNSRLQGISLGVKYHLSL"
     gene            complement(11878..12321)
                     /locus_tag="PK252_12450"
     CDS             complement(11878..12321)
                     /locus_tag="PK252_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15562.1"
                     /translation="MKKYISIIAILLISFGSIQNNQAQCKGFAKKVCKVELNPYTHDG
                     NFHAAILTEGEEAELYKTFYSDTEYRIAVCGSEQLPTIEFKVLDPDNKVLYSNKENNF
                     ARTWDFKLQSSQQLKIVVKVHPSGKNVENVQSGCVSIMFGFKEAK"
     gene            complement(12423..13325)
                     /locus_tag="PK252_12455"
     CDS             complement(12423..13325)
                     /locus_tag="PK252_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15563.1"
                     /translation="MKNSKILLIALTIGTFQIVSSCRSCNKDVANQEVAVEVDEEIAE
                     NINQAKKIFYSLPSPLETAMLIKTAGATYNERLLNPLSNVSNYTTNKSMALNLGVYTT
                     DLSFASLFDQTQTAIDYMQAAKKMADGLGILDAINSNTIQRLEENINNRDVIMDIISE
                     TFMASSSFLKENDRTAVASIVLVGGWVEGLYIATSLVNPDNFKNSKLVERIVDQKLSF
                     DIVLKLLDENKDNPDVASLIVEMNELKKSFDKIEIKSSKIEAVKNSSTNVTTLKSETS
                     SNINQAVFKELTDKVKTIRSNFIL"
BASE COUNT         4300 a         2283 c         2729 g         4137 t
ORIGIN      
        1 ttaataccat acacggcgaa ggttgcaaaa ctgttaccgg ttattcgcaa gaagaattta
       61 ttagtgatca atacctgtgg ttcaatatta ttcacgaaga agacagagat gcagtcataa
      121 accaggccaa aattcttatg cagaatattg agccatcatc aatcgagcac aggattatcc
      181 acaaatcggg tataataaaa tgggtacgca atacccctgt tatcaggcgc aacgaacagg
      241 gcgaaatgat aggctatgac ggcctgatag cagatattac agaccgaaaa atggctgaaa
      301 tagctttaaa agaaactgag cgcaaaattc ttaatgctac tattgaagca gaagagaaag
      361 aaagagccca tattgccaga gaattacacg atggaatagg cccattactg tctactatta
      421 aactttatat tgaatggctt aacaagccag aacttcaaac cccaaaagaa gaaattgtaa
      481 acaatgccaa caaaacagtt gcagaaacaa tatctgccat aaaagaaata tcacaaaagc
      541 ttagtcctta cattgttacg aattttgggt tagaacatgg cctgaaagaa tatattgata
      601 aactaaaaga atcttgtaac attgttatta attacgaatc aaacctcaaa atcaggctct
      661 cgtctgaaat agaagtcacc ttataccgga taatccttga atgtattaat aatactataa
      721 aatatgccga ggcaaaaaaa atttacataa ggctcaatgt gtttgagcag caactatttg
      781 ttgattatga ggatgatgga aaaggttttg attattcaac tttaaaatct gatggcaatg
      841 gacaggggct tatcaatatc aaaaaccgta ttgccagcat gggaggaaag ataactatag
      901 aaagcaggcc ggggcatgga ttcaagatga aagtaataac aaatatttag catttattat
      961 tcaaatatta ggtaaaaaaa tattatcttt agataggtca aaaagataag ggcatgaagg
     1021 ggaaacgctt aaaaatactg atagttgatg acagttcttc atttaggagt gcggcagcta
     1081 tgtttttgac caacgaacta aactgtgaga tcgtaggaga agcagctaca ggtagggagt
     1141 ttcttaaaat gataaaaaat ctggatgttg acatagtgct gatggatatc cagatgcccg
     1201 acatggatgg aataagcgct acaaaagaat ggtgcctgag gtatccacat accaaggtgt
     1261 tggccattac aatgtttaca gacaaagcct ttttgcttca gcttattgag gcaggtttca
     1321 aaggatgtgt atttaaagcc agcttcttta gcgagatatt aacagccatt gaagctatca
     1381 tggatggtaa attatatttt gaaaaagata tgccggtttc ttagcttttt acaagcctta
     1441 ccattcgttg tttttcaata tttacaatat ctttgtaagt attataaagg tttttacatg
     1501 aacaatactc caaattatgt catagccatt gatggctatt cttcttgtgg caaaagtact
     1561 tttgcaaaag ctattgcagc ccttctgcag tatatatata ttgataccgg ggcaatgtac
     1621 agggctgtaa cgctgtatgc cctaaataat aagttaatta caaatggtgt tgcaaataag
     1681 gaagcggtaa tcgaacattt gccaaaaata aatgttaggt tcgaaagaga aacggatggc
     1741 caggtaaaaa ctatactcaa tggtgaggat gtttctgaaa ccatcagaaa tcttgaagtg
     1801 tcaaaaaacg taagtgctat aagcgccata aaggaagtga gacagaagat ggtagaacta
     1861 cagcggtcga tgggtaaaca aaaaagggta gtgcttgatg gcagggacat aggaacagtt
     1921 gtttttcctg atgccgaaat aaagatattt atgacagccg acccaaaagt cagggcacaa
     1981 cgtcgttacg acgagctgat agcaaaaaat atgcctgctt cattcgacga agtttatcaa
     2041 aatataatag acagagacca tttagatact acacgcgcag aaagtccatt gactaaagcc
     2101 gatgatgcaa tagtacttga caacaccctg ttaactccac aggaacaggt ggattggttt
     2161 ttagaactgg ttaaatcaaa aggatagttt ttaatctaaa tagtagaatt atggcacata
     2221 ttgtatggga tgagaagatg agtgtccagg tagggctgat ggatacccag cacaaaaaac
     2281 tgatagaagt tataaacgac ttttatagca gcatggggag caataatagc aaggaaaaaa
     2341 cccttgaagt gataaaggcc atgaaagatt atacgttttt tcattttaat gcagaagaga
     2401 aacttatgca gcaaaatgcc tatccggatt ttgaaaagca taaaaaagag catcaggcat
     2461 ttatagataa agttcacgac atggaagaaa ggtacaagtc cggaaagctc atccttaccc
     2521 tcgaagtggc cagtttttta aaaacatggc ttaccaacca tattatgcag gtggataaga
     2581 aatacggtga ttacctgaat gctcgtggca ttcgctaatt tctggataaa acaaaatgaa
     2641 ggttaccatt gacgaaaagt ctggtttttg caatggcgtt gtaattgcaa taagccacgt
     2701 agaagaagag ttgaaaaaaa gcgggcattt atactgcctt gggcagattg tgcacaataa
     2761 tgtagaagtg gaaaggctcg aaaaaatggg ccttgaaaca attaactacg aacaatttaa
     2821 gcaattaaag gattgtaaag tgcttatccg cgcgcatggt gagccacctc tgacatacca
     2881 gatagcaaag cagaacaaca ttgaattgat agacgcttcg tgcaaagtag tgttgaaact
     2941 tcaggagcgt attaggaatg agtacaataa tactgttgag aaagcccaga ttgttattta
     3001 cgggaagaag ggccatgctg aagtaaacgg tctaattgga cagacaggag gagatgctat
     3061 tgtagtaagc agtgatgctg atttagaaaa tatagactat actaagccgg ctcatttgta
     3121 ctcgcaaacc acaatgagta ttgaaggctt tcaacaattg gccgataaaa taaggcagcg
     3181 tatgcagcag gctcagggta aggagacggt ggcattgtat atacatgata caatatgcag
     3241 gcaggtttcc aatcgtgcac ctcatcttcg ccagttttca aaggataatg atgttatcat
     3301 ctttgtcagt ggccgagata gctccaatgg caaggtgttg tacgaagaat gcagacaggt
     3361 caatgctaat acgcactttg tttcggatat agccgatatt cagagcaatt ggtttgtcaa
     3421 taaacaaaca gtcggcattt gtggtgcaac ttctacgccc atgtggttaa tggaaaaagt
     3481 tgctactttt atagctcaaa ttaaatgagc tgtcgattaa atgctttatg ccgacataat
     3541 actgccgttt ccgttacaac aatattttac ctatactgtt cctgttgagc tttgcacgca
     3601 ggtagttatg ggagtgcgtg ttattgtgcc tttcggcaaa agtaagtttt acaccggtat
     3661 tgtagcatcg ctatacgaaa accaggctgt caattcgcaa attgaactca aaagcattgt
     3721 ttcggttgtt gataacaagc ctgtcattag tgagtaccag ctgaggtttt ggcagtggat
     3781 ggcctcgtat tacatgtgtt ctccgggcga ggtgatgcgg gctgcattgc ctgcagggct
     3841 gaaactcgag agcgagtcgt ttgttgttgc aaacggtcag tttgatggca ttgatgagct
     3901 tagcgagcaa gaaagcactg tatatggctt tattcaaagc aaaaagcgca ttacgctcga
     3961 agacgtttat aaatcggtga aaattccccg tgttgttagt gttgtaaaga aattgtacga
     4021 gcaaaaggcc atttttattg atgaaaaaat agttgaaaaa taccagccaa agcaacagac
     4081 ttacgtaaga ccttgtaata cagatgctac atttgacgag actatgaaaa tgcttgccaa
     4141 atggcccaag caacaacagt tatacgaaag gttgctgcaa cttattgata ctaccaatgc
     4201 agatgtcgaa atcggtgttt ctcaaaagct tttgctcgat tctgaaggct tcagcagttc
     4261 ggtagtaaaa acgctcgaaa agaaagggtt actttctgtt tatagccgcg aaatgtcgcg
     4321 tttcaaagaa tcgactaatg aaaacgaaac gttggttgag ctttcagcag ctcagaatat
     4381 tgcaaagtca gcaatagaaa agtatttcga agataaaaat attgtattgc tccacggtat
     4441 tacctcgagt ggcaaaacag agatatattt tcaccttatt aaagatgttc tcgaaaaagg
     4501 caaacaagtt ctctttcttt tgccagagat tgccataacc tcgcagatga taaaccgtat
     4561 gcaacggtat tttaagaata aagtaggagt gtttcactcg aaatattctg acagtgagag
     4621 ggtagaagtg tggaaacgcc ttatggaaag caatgctgaa ggcatacaaa ttattcttgg
     4681 tgtcagaagc tcggtatttc tgccatttca gaacctcggc ctggtaattg ttgatgaaga
     4741 gcacgaaaac acttacaagc agaatgaccc tgcccctcgt tatcatgcca gggatgcagc
     4801 catggtactt gcccagatgt acaatgccaa agtccttctg gggtctgcaa ccccatcggt
     4861 agaaacatat tttcatgcta catcaggcaa atatggatta gtaaacctta atgagcgctt
     4921 ttcaggtctt gaattaccta aggtagaact cgttaacctc aggattgcaa agaaaaaagg
     4981 caataccact tcgtatttct ccaacaggtt gttgcaagcc attgatgaaa ccattgcaga
     5041 tcagcgtcag gtgatattat ttcagaacag gaggggtttt tcgccttatt tacaatgcgc
     5101 ttgtagttgg atacctcgct gtaggcactg tgacgtgact ttgacatatt ataaaaacca
     5161 gaacaagctt gtttgccatt attgcaactt tgtagccaat gtacccgata agtgcattga
     5221 atgtggtgga acagacataa aaactatggg tttgggcacc gaaaaggttg aagacgagct
     5281 gaagttgatg tttcctcaat atcgtgtggc ccgtatggac ctggattcaa tccggggtaa
     5341 gtttgctcat gagcatatca ttgcagactt tgagaatggc aatgtggaca ttctggttgg
     5401 tacacaaatg gttactaaag gattagattt tcaacatgta ggattagtgg gtattctcaa
     5461 tgcagatagc ttgctcaatt atcctgattt cagggcttat gaacgcagtt ttcaacttat
     5521 gcagcaagtg agcggccgtg ctggtcgaaa aggcaagcaa gggctggtgg ttattcaaac
     5581 ctattcgccc gctaaccccg aaatccggtt tgttttaaaa aatgattatc aggctcatta
     5641 tcaattccaa ataacagaac gaaaggaatt tttatatccg ccttataccc gtataattca
     5701 ccttatgctc aagcatgtgg acgagaagct aacttgggat gcggccaatg tccttgcaaa
     5761 actattgaaa caaaagcttg gcaacagggt tgctggcccg gctcgtccat tggttggacg
     5821 catccagaac caatatattg tgggcatcgt gataaagctg gagcgaacac tcgatttggc
     5881 aaaaataaaa ctcgaaataa gccacattat tgcagtatct atggctaact ttaaggcagt
     5941 gcgcgttact attgacgttg acccgatgta aaaaagtttt gccgcctaaa ttgcatcata
     6001 acgggcaaaa tgtttaacat tgtgaaaaat ttttcaaagt ggtttccgat aatttaaaga
     6061 cttttttcaa agacctgaca tcaaagcgaa tacttattat tggcgatgtc atgatagatg
     6121 cttatatgtg gggcaaagta gacaggattt ctcctgaagc tccggttccg gttgttacct
     6181 gtacaaaaca agataacagg cttggaggag ctgccaatgt tgctatcaat attgctgcta
     6241 tgggtgcaaa ccctatcctt tgcggtatta ttggaaatga tgataaaggg actttgttcc
     6301 taaagttgat gcaggataat catctgtcta atgccggtat ctgtatagta aaaggacgaa
     6361 agacaacaat taaaacccgt atcatcgggg gaaaccaaca tttgctccgt attgatgacg
     6421 aaacaaccca acctttggaa ggcgctgaaa tagttaactt ttcacaacac ttgttcgatc
     6481 tgatcaatga attaaaagta gatgctatta ttttccaaga ttacgataaa ggtttattga
     6541 cacctgcttt aattaatgaa gtaattcatt ttgccaataa aaaagatatt ccggtattgg
     6601 ttgaccctaa aaagcgcaat tttcatgctt atcatggggc aaccttgttt aaaccaaatt
     6661 ttaaggagtt ttgtgagggt ttgaaagtag aactttcaaa agaaaatttg caagatgtat
     6721 atactgttgc caaagatttt ctaaattccc ataatatcaa gatactatta ttaaccatgt
     6781 ctgaaaaagg ggtaatgatt tgtactacag aaaactttca tcatatacct gcccatgtga
     6841 gagatattgc agatgtgtct ggtgctggag atactgttat cagtgttgca acattaggca
     6901 tagcttgcaa tctcgattta aaggaagttg ctgaggtgtc taatctttcg ggaggccttg
     6961 tttgcgagaa agtaggcgtt gttcctgttg ataaagcaca attagaaaga gaagttagaa
     7021 aatatttgca ttagaaattt gcatttttct ttatctttgt agcatctaat agctaatcgg
     7081 ggtatagtcc cgcctggggc aggacgccac gtaaaaggta aaagataatc ggggtatagc
     7141 gcagttggta gcgcgccacg ttcgggacgt ggaggccggg cgttcgagtc gccctacccc
     7201 gacttatatt acagtttagc cccggttttt tcgcccattt ttacaagcgt ttcaattcct
     7261 tttacagaat gttccaaaat atccgggtcg ggagtgatgt attgtttctt aaatagctgt
     7321 ataatagtag aaccaccata ttcaaaccat cctttttctt cgcctctgcg gaaagtatgg
     7381 gcgtgacgtt tcctttggac aattttacct accaataatg ccccaacttc caattgaaca
     7441 actttctcga aattttctgt ttctaaaact gtgagttcac gataattttt agataacaaa
     7501 ttttttgtag ctgaaagggc aatagggttt accgaatgca aaactcctcg taagcgtttt
     7561 actccagttt gtgtaccatt gtctatataa caaaacctgt gataatcaga aggggcgaga
     7621 cgataaacaa aacaccagcc atcatggtat tcctgagcta tcttcttatc ctttaccaag
     7681 tctaacagtg agtaccaata accttttacc ggcaaagctc cagtattggc taagtcgaat
     7741 accataagtc ttgagtcggc tggagatata agatgagaag gtgtttcatc aacaggacga
     7801 gcccctggca ctaactcccg aataaagaaa gcattaaaat tcctgaatga ttcaatgggc
     7861 tgctttattt cagaagtatc tatgttgtaa tgatggataa attttttaat taaataccgg
     7921 ctttgacgac ttttaacagc ttgcccgtat atttttgaca ccatcttctt attgaggata
     7981 ctgtggatca gggttttccc aatttttgtt ttgtacaaaa atgccaagcc atctgcctga
     8041 taagtctttt cagattcaat atgttgtgtg tttctattat aaagtaatac ttgtttcatt
     8101 tgtgtaaaat tttaatttat tgaaatacag ttatttaagc tgtcgtgttt gatatttagt
     8161 gtgtagaagc aacccctttc caataaccgg gataggtgtt tcatctgaaa gaatatgttt
     8221 tttcagcaaa aataatggta ttcattagat gcatgttgaa tttaaaaaaa aaaatgcttt
     8281 aaaggctcat aatagttaat tagttgtttt tatccaattc attacttcac aaaaaaatta
     8341 atcggatgtc agctttttta tgatgtccga atgcaaatca acggttaaaa atgaatttaa
     8401 agaacacttt caaaaataca aagcttttgt ggtttacctt ttatatattg aaaaaaccta
     8461 tatctttgtg aatctaaaaa ctaaaaacta tggaaaacaa taatggttcg ttgctcaaag
     8521 caactatgaa ttatggttta ataacaggat taatcgttat agtgtattct cttttgcttt
     8581 atattattgg tttacattta aaccaatatt ttgggtacct gacaattgta attatggctg
     8641 cctgtatttt catctttacc aagcaatata gggataaaca aaacgaaggt tttatcagtt
     8701 ttggcaaggc ctttcaatta ggcattttga taggggtgtt tgctgctatt ttgttgtcat
     8761 ttttctctta tattgaagtt acttttatag accctactat agttgaaaaa cagttggata
     8821 tccagcggga aaagatgctt gcaaaaggga tcagcgaaga acaagttgaa atggggattc
     8881 aaatggcaaa aaaaatgatg acgcctgcaa tgttattcat tgtgtcaata ttgtctttta
     8941 ctttttgggc tgcaatcatt tcattaatta cttcagctat tttgaaaaag aacagtagta
     9001 attaattttt tttcaaaatt tgtatcattt agttttaagc aatggattta tcaataattg
     9061 ttcctgtata taacgaggaa gagtctattc ctgagttggt tgcctggatt gacagagtag
     9121 cattagccaa ccatctttct tatgaagtgg taatgattga tgatgggagt actgataatt
     9181 catgggagtt gatagaatct gttgcccagg caaataaaaa catcagggcg attaagttta
     9241 gacgcaacta tggcaaatct ccggccttgc aatgtggttt tcaggctgca aagggagatg
     9301 tggtgattac catggatgca gaccttcaag acagccctga cgaaattccc gatttgtaca
     9361 agatgataac cagagatggg tacgatatgg tatctggctg gaaacaaaaa cggtatgatc
     9421 ctcttgcaaa acgtttgcca agtaaaatat acaatgctgt agcccgcatg gtgacaggga
     9481 ttaaacttca cgactttaat tgtgggctaa aagcatataa aagtcgtgtg gtaaaaagta
     9541 ttgaggttta tggagatatg caccgcaaca tcccatacct ggcatatcag gcaggtttta
     9601 aaaaaattgg ggaaaaagtt gtgcaacatc aaaagcgcaa atacggatac tcaaaatacg
     9661 gctggcagcg ttttgttaca ggctttcttg atttgatgtc gctggtattt gttacaaagt
     9721 ttggtaaacg ccctatgcac ctttttgggg tattaggctc gctgatgttt attgctggtt
     9781 tatcctgggc tgcattttta ggaatagata aactttatgc tgtaagccac aatttgccgg
     9841 ctaaattatt aactcagagc ccctatttct atttagcttt ggctgccatg attattggca
     9901 ctcaactgtt tcttgccggg tttcttgccg aattgattgt tcgttcttca gcagacagga
     9961 atacctacct gatagataaa tcaattgatt gatgaaagca ttgttgttag gtccggctta
    10021 tccattaaga gggggcattg ccaactttaa cgagtcattg gcagttgctt ttatcaaaaa
    10081 ttcgatagat actcatattg tatcttttta tttccaatat ccatcgtttc tttttccggg
    10141 caaaacccag tttacaaaaa ataaaccggc acatttcatt gagataaaat caatacttag
    10201 ttctataaat ccgatatcgt ggtataaaac tgccagatac attgagcagg agaatccgga
    10261 tgtaattatt gtccaatatt ggatgccttt tatggcacct gcattgggca gtgtacttag
    10321 attgattagg cgtaaaaggg cgcctaaagt aatagctatt gtacataatg ccaagcctca
    10381 tgaaaaacaa cctggggcaa caatgcttac caaatatttc ctgaatgttt gtcatggtta
    10441 tatatgtctt tctaaatctg tgttgaaaga ccttgacgag tttactgtta atcccaataa
    10501 aatattcacg cctcatcctg tgtatgatat ttttggtgat aaagtcccta aaaatgaggc
    10561 caggaagtat ctttccattg gtttacagga aagggtcatt tgtttttttg gtattatcag
    10621 aaaatacaaa gggcttgagc tgttattgaa tgcaatggcc aatccttccg taagagattt
    10681 gaacctaaag ttgattgttg ccggggaatt ttacgataac aagcagtttt attatgattt
    10741 ggtgaagaca ttgaatattg aatccaatgt tgtttttact gataggtata tacctacctc
    10801 tgaagtaaaa tactacttct gtgcatcaga tatgatagtt cagccatact cagacgctac
    10861 acaaagtggt gtaacgcaaa tagcctataa ttttgagcgg cctatgttgg tgactaatgt
    10921 aggaggcttg gcagaaatag tattccacaa ccatactggt tatgtaacag aggtaaatgc
    10981 agagtcaatt gctttatcaa tttctgattt ttacctcaat catagggaag aagagatgag
    11041 cgccaatgta gcaatggagc aatatcgttt ttcgtggcac aatatggtgg acgcaatctt
    11101 aaacctcaca gaaaacttgc aataggcttt atttataaac ttaggtggta tttgacccct
    11161 aaagaaatac cttgcaaacg tgaattgtca atatattgat attctttttc gtataacgaa
    11221 gtgagagaaa aatgataatg caacaaaacc gacaattctt taataatcct gtattctcct
    11281 ccaaaaacca atgaagccat atattttgac aagccctttt gataatcttt attattgatg
    11341 gcaagcgtgt tatttttttg tatgtaatat ttatattcct gattgttata taaaagtgct
    11401 gacaactgaa cgcctccgtc aatatttaaa ttgtcaataa tttcataatg gtaaacagcc
    11461 ggaatattta accatacaag atttgcggca caagtaggat tgatagcccc agtttcaatg
    11521 acaaatgaat tgtttgttat cagagggttt atatcacctt gttccacatt tctttcgaaa
    11581 tatttgagat tgtacgaatg ccagttaaca cctaacccaa tcgaaaagtc atttttattg
    11641 ataagtgtaa gtttataaaa agcactcaaa gcaatgcctg gtttttcttt gtattccagt
    11701 tgctgcttgg taatgttgcg atctatcggg tttgaataat attgagattg gttaggtaaa
    11761 aaataataaa atgaagataa ttgaaactca aagtcttggg ctgaaagtgt aattgtaaat
    11821 acagcaatca gcagggttaa gtattttttc ataattgggg gaagaaataa ttagttttta
    11881 tttagcttct ttaaagccaa acatgattga tacacagcca ctttgtacat tttctacatt
    11941 tttgcctgaa gggtgtactt ttacaacaat tttaagttgc tgacttgatt gtaatttaaa
    12001 atcccaagtt ctggcaaagt tattctcttt gttagaataa aggactttat tgtccgggtc
    12061 gagaacttta aattctattg ttggcaattg ctcagagcca cagactgcaa ttcggtattc
    12121 agtgtcagag taaaatgttt tatacagttc agcttcttca ccttcggtaa gaatagcagc
    12181 atggaaatta ccatcatgag tatatggatt taattctact ttacatactt ttttggcaaa
    12241 tcctttgcat tgggcttggt tgttttgaat gcttccaaaa gatatcaata agatagcaat
    12301 gattgatata tattttttca tccgtaaata attttttaat aaggtcggat aaaactcgca
    12361 tgatgaattt ttatccttat tgtgtttaac gaatcatgct cgtagttagt ttcctaattt
    12421 tcttataata taaaattact tcgtatagtt tttactttat cagtcaactc tttaaatacc
    12481 gcttgattaa tatttgaaga cgtttcagat tttaatgttg taacatttgt actggagttt
    12541 tttaccgctt ctatctttga tgatttaatt tctattttat caaaagattt ctttagttcg
    12601 ttcatttcta ctataagtga agcaacatca ggattgtctt tgttttcgtc aagaagcttt
    12661 aatacaatat caaaagataa tttctggtca acaatccgtt ctacaagttt actgttttta
    12721 aaattatcag ggttgactaa agatgtagca atatataacc cttcaaccca acctccaacc
    12781 aaaacaattg aagcaacagc tgtgcggtca ttttctttta aaaagctgct tgaagccata
    12841 aatgtttcag aaataatatc cataatcaca tcgcggttat tgatgttttc ctccaaacgt
    12901 tgaatggtat tactattaat tgcatctaat atccctaatc catcggccat tttttttgca
    12961 gcctgcatat agtctattgc tgtctgggtt tgatcgaaaa ggcttgcaaa actaaggtct
    13021 gtagtgtaaa caccgaggtt aagggccata cttttgttag tggtataatt cgaaacgtta
    13081 cttagcgggt ttaataacct ttcgttatat gtagctccgg cagttttaat gagcattgca
    13141 gtttcgagcg gcgaagggag cgaatagaat attttcttgg cttgattgat attttcggca
    13201 atctcttcat caacttctac agcaacctct tggttagcaa catctttatt gcaagagcgg
    13261 cagctactaa caatttgaaa tgtccctatt gttaaagcaa tcaataaaat cttggagttt
    13321 ttcatgtcta ataataaatg gttgaatagt aatgctaatt aataaaagta aaagtaagtg
    13381 ttttttttat aaaatacaac actagtgtcc cattaaaatc gggacgttat taaaaattaa
    13441 acaaatttg
//
LOCUS       DAOSPF010000078        12818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4883_length_12818_cov_7.011596, whole genome shotgun sequence.
ACCESSION   DAOSPF010000078 DAOSPF010000000
VERSION     DAOSPF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12818
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_12818_cov_7.011596"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PK252_12460"
     CDS             complement(<1..1130)
                     /locus_tag="PK252_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT15564.1"
                     /translation="MNYSFSVRMNGVPKSFIREILKVASDPSFISFAGGLPNKDLFPV
                     EGLRKATEKVFKTDGKNILQYSTTEGYKPLREFIANRYKTHKGIEVNPDNILITTGSQ
                     QGLDLLGKIFINEGDNVLIEEPAYLGAIQALSVYKPVFTAIPLNHEGMDVQILKKALF
                     SSRFKLFYAVPNFQNPSGITYTSENRKEVAGLIEKAGIVMVEDDPYGELRFLGEQEAS
                     FKTLIPDNVMMLGSFSKIVAPSFRLGWVVAPDEVYEKLVVAKQATDLHTSYIAQRIVY
                     QFLIDDDLDKHIQNIRDAYGKQREAMVDAIKKLMPKEVKFTLPEGGMFLWLTLPNHLS
                     SMTLFNKCIERKVAFVPGNPFYVNKNDVSTMRLNFSCSNADE"
     gene            complement(1297..3291)
                     /locus_tag="PK252_12465"
     CDS             complement(1297..3291)
                     /locus_tag="PK252_12465"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT15565.1"
                     /translation="MKKLVTIALIFGLSVNVINSQANKNVAEIDASKAKQKISKYIYS
                     HFSEHLGHCIYEGIWVGENSTIPNKNGIRTDVVEALKKIQIPSLRWPGGCFADEYHWK
                     DGIGPRNLRPSMINTNWGGVTEDNSFGTHEFMELCNQLGCEPYISGNVGSGTIQEMSQ
                     WVEYLNSDNVSPMTQLRKQNGRDKSWGVKFWGVGNETWGCGGNMKPDYYSDLALHFSS
                     FLKNYGSNNLRKIMVGPSDEDYNWTETVMKEIGTNMWGLSLHSYTWANTKSATDFDEA
                     GWFDVLNKTLRMETLVTKHSEIMDKYDPAKSVALAVDEWGTWYAVEPGTNPGFLYQQN
                     TLRDALVAGINLNIFNNHCDRVRLAAIAQVVNVLQAVILTDKEKMILTPTYHVFDMYK
                     VHQDALLVPTSLQCEDYTFNQKSVPSLNMSTSIDKNGVMHITICNLHATKEQVLTCNL
                     KGYDVSKITGEIISADKLNAHNTFDKPNEIGIKAFTKFKTTKTGVDVTLPAHSVVMLT
                     VDGKRQVSDMTIKPKNPKAGLKYDLYEGTWTKLPNFTTLTAVKQGVATEMDVIKGFPE
                     LNFGVVYKGLIKIEKDGMYDFGLSSDDGSRLIINDECIISNDGLHGMVEMQGSSYLQK
                     GYHKFEVQFFQGGGGAGLQLFIQGPDMNKQLIPASMLWYE"
     gene            complement(3562..4986)
                     /locus_tag="PK252_12470"
     CDS             complement(3562..4986)
                     /locus_tag="PK252_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15566.1"
                     /translation="MKTLMIKLMFLTVCTCLCSTAIYSQTLKDKSISEVYYLYPNNPL
                     PSFVSTFYIETSRLGKDEECFNTVNDPKYAWTRPIEGYTQVYNESEADIKISFERLNY
                     KIVRKEAIDTVIERSEKKDYERFYRVFFTIRFELVVKGKKMPGVIFKEPININSFFET
                     RSIAAKESLEKQISQTEIDNTLKNYISTRIFSLLRNYWGKEQKALFRMDLYTAKGKHN
                     YDDLDDAFKQFKTVMKEDIKGGLNEEQKKKIESCVAVWGKALTEKDTVNPKARITKEI
                     YCGLCVNLAVANAWLGNIENAESYYYQSLGYNNDKDFKNNLKQVRKWLLDYESRYIKN
                     HPEYKIMPLLEGKWRLIKYISDEKVDLNKDGKESNDILSELDSYKKHKIFDISANRIL
                     NVIEGDAPACEAKTTKYYYKVSKNKINERKYLIFDDNEDIDESLASIFQITSLNGENL
                     TIKGMAYLTGDTTSEVEFTFSKTK"
     gene            5317..6303
                     /locus_tag="PK252_12475"
     CDS             5317..6303
                     /locus_tag="PK252_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HOT15567.1"
                     /translation="MKIRLGKNLLTIFMSGAVLLTPLLFPPAAAQKTVCANEIGTEGG
                     YSYELWKQKNDAESCMTLKDGFLFSCEWKESSENFLARRGFKYDTTQTPSQIGNFVAS
                     FACNYNPSGNSYLAVYGWTVSPLVEFYIIESWGTWRPPGATSKGTITVDGGTYDIYQT
                     TRENQASIKGTATFQQYWSVRRTKRTSGNISIFKHFEKWEEMNMPLGKLYDINLLIEG
                     YKSSGNAEFTSATIDTNGDPTAVNAIENNTKQNSPLSVSVDPSLNILDVNVLKASGSP
                     IKANIYSISGTLLKKVELVSGNNKIDINDLQKGLYLLSTSINNEVIAQKFLK"
     gene            complement(6384..6929)
                     /locus_tag="PK252_12480"
     CDS             complement(6384..6929)
                     /locus_tag="PK252_12480"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOT15568.1"
                     /translation="MNSKFLFLRFLLFVCIGLLFSSCMNVSNNGHDILQNGFDQNHLQ
                     INESSSIKKVRGQVLYLPVYSNIPYQEKNRIFDLSAFIAIHNTDFTKTVKITKVLYFN
                     NDGKFVRSYLDKDSVLNPLGATNFFVPEIDKSGTGANFIIEWQADTLVNEPLVESVMI
                     GLTSGQGVSFLSNGKVLREIR"
     gene            complement(7223..8281)
                     /locus_tag="PK252_12485"
     CDS             complement(7223..8281)
                     /locus_tag="PK252_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOT15569.1"
                     /translation="MVDFIYSNLFFFIFGIVILSVLLLDLLVIGKNKHEISFKESLLW
                     TSVWVCLAIGFYFFIRFYGDHIHNITTMDELVNYIRLYSPHVKLNTTDFNEALKIYRE
                     NAATDYIAGYFLEYTLSIDNVFVILMLLTGFSVEKKNYKTVLFWGVLGAIVLRFIFIF
                     AGSALIVKFNWILYVFGAFLVYSGIKMYLDRNNEETIDVGEHKLVKFLSNHISIYPQY
                     IGGKFWKRIDGKFFFTPLFLVLVMIEFTDLIFAVDSIPAIFSITRDPYIVFFSNIFAI
                     IGLRSLFFLLIKVVDLFHFLKVGVAFLLAFVGVKLLAHHYLEMIGFKNVYSLYIILAT
                     LAISILASIIFPKKKAVE"
     gene            complement(8307..8960)
                     /locus_tag="PK252_12490"
     CDS             complement(8307..8960)
                     /locus_tag="PK252_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOT15570.1"
                     /translation="MALSDYEQRRFASYISFGKIGLKGMVALKSSKILIAGLGSVGIP
                     AALYLLSSGIGKLGLCDYQTISENDFYKQTIFGTGDLGKLKTIVLKEKLAHLHPNAQI
                     TIHNIELTCENLVRFAAGYDIVLNCTNMLDNDAIFAALPENKIVLNVYCRGFSAFVWS
                     GMLNTSLKTEIKDTEKAAGFSFVVGPMAGIAGSTLANIVFKHLSGIETMVKHLQIKL"
     gene            complement(9023..9391)
                     /locus_tag="PK252_12495"
     CDS             complement(9023..9391)
                     /locus_tag="PK252_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15571.1"
                     /translation="MDKVIFFIDDDKMILNLMQYTFSNRDNCKVFAFSKGEDCLPFLD
                     KNPNIVVIDHSFVNNSIFNTGLEIIDKIRETYAGASFIVLTGSNDSAIRKQYEQRGVT
                     SFINKDSFFIDILIEKIACI"
     gene            9506..10672
                     /locus_tag="PK252_12500"
     CDS             9506..10672
                     /locus_tag="PK252_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT15572.1"
                     /translation="MEKNLWNKSFSLLCSVNFLVAFTFYAIMALLPGYLNSHVKIDSS
                     QIGILMSSFTLSALLIRPFAGYLIDNHRKKLILGAAILLFATSFLLYLFSATFVSIFV
                     VRFIQGIAWGAFSSTASVMVIDVLPVERRGEGIGLYGVFMTGAMAISPLVSIFALKFT
                     SYGNLFIVSFFIVFGCLGLILKVKSFNTFSNKKFTFHEVINVKVIPLSLFIMIATMAY
                     GSVLSFITLYAADRHYNNVGFFFLAQAIGLTLMRFLAGKAYDRNGPKVLTILSFASLI
                     TGFLLMIFSGNEFLFCFSAIFIGMGYGSLFPASQAMINELVTAKDRGTANATFLTFFD
                     TGVGLGMISTGFLSKLFGFQTAYLMCVAYLLIALTVFLIIGLKYYLKHRITNAL"
     gene            10903..12051
                     /locus_tag="PK252_12505"
     CDS             10903..12051
                     /locus_tag="PK252_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT15573.1"
                     /translation="MKKLAILIALSFFINSFGQQYSKKWADINYAGDAKNYHLMDIYL
                     PKAVKTSYPVVVYIYGSAWYSNSSKGADMNTIGKALLDAGYAVVTPNHRSSSDTLYPG
                     QINDIKAVIRFVRGNAATYSLDTAFIGISGSSSGGHLAALAATSVGVKKFTVDSTTMN
                     IEGNVGKYNTFSSKVHAACDWFGPTDLLKMDSCRGSSMYAEGQSPEETLIGGTKKALR
                     NKFVMLSPTTYIDSTDCPFLIFHGDADNVVPYCESELLYNALQLYGVQSEYVRVPGGQ
                     HYTGTHTDANIKKMVDFFNKISKNNISAVIALNNTVKTYVANNTLTIEGISDFSVYQY
                     EIIDISGKKLRSNAFTTSKIDIASLKNGVYFFKLQSKDGNSMIDKFVK"
     gene            complement(12327..>12818)
                     /locus_tag="PK252_12510"
     CDS             complement(12327..>12818)
                     /locus_tag="PK252_12510"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT15574.1"
                     /translation="GLNLPVTKWYRISANINAYTNRFYSKRSSDNINNDSYTMNVWSG
                     HYFTLPKNYVLEITSWYQAPQAYGVFNLSGQYNIGMGVQKQILKQRGQIKLFVRDIFN
                     SSKETVKMWSNGNLILLNRNKWDSRQFGFNFSYRFGSDDVKPSRERKTGLDDELNRVK
                     TGK"
BASE COUNT         4078 a         2490 c         2227 g         4023 t
ORIGIN      
        1 atttcatcag cattggagca ggaaaaattc aaccgcatgg tggacacatc atttttgttg
       61 acataaaacg ggttgccagg gacaaaagct actttacgct caatacattt gttgaacaag
      121 gtcatagaag ataagtgatt gggtagtgtc aaccacaaaa acatacctcc ctcaggcaga
      181 gtaaatttaa cctctttggg cattagcttt ttaatggcat ctaccattgc ttcgcgctgt
      241 ttgccataag catcgcggat attttggatg tgtttatcta aatcatcgtc aataagaaac
      301 tggtaaacta tacgttgggc aatataactg gtgtgcaggt cggtagcttg tttggcaaca
      361 accagctttt catacacttc atccggggca accacccacc ctaaacggaa tgaaggggct
      421 acaatctttg aaaaagaccc taacatcatg acgttatcag ggataagagt tttaaacgaa
      481 gcctcttgct cgcccaaaaa cctcaactcg ccataggggt cgtcttctac catcactata
      541 ccggcttttt caatcaggcc agctacttcc ttgcggtttt cagacgtata agttatcccc
      601 gatggatttt gaaaattagg aacagcataa aaaagtttga aacgagaaga aaacaaggct
      661 tttttcagta tctgtacatc cattccttca tgattgagcg ggatagctgt aaatacaggt
      721 ttgtaaaccg acaaagcctg aatagccccc aaataagcag gttcttcaat cagtacattg
      781 tccccttcgt tgataaatat ctttcccaag aggtctaacc cctgctgcga accggtagta
      841 ataaggatat tatccgggtt aacttctatg cctttatgtg ttttatacct gttggcaata
      901 aactcgcgca gaggcttata accttcagtt gtactatatt gcaggatatt tttaccgtca
      961 gttttaaaaa ctttttcggt agcctttctc aggccttcaa caggaaataa gtctttattg
     1021 ggcaagcctc cggcaaaaga gataaacgat ggatcggaag caactttcaa tatttcgcgg
     1081 atgaaagact taggtacacc gttcatccta acagaaaaac tatagttcat aatgcaatct
     1141 aaagggtttt tagtattgca aagatacata aaaaagggga aagagaaaaa gttcaaaaaa
     1201 aaggaaaaac ccgggagcta tcgtttcact tccgggtttt tctcttacct ttataatgaa
     1261 aaaagtaatt cttatacttt tgtgccagca gaaattttac tcataccaaa gcattgaagc
     1321 cgggataagt tgtttgttca tgtccgggcc ctggataaat agctgtaacc cggctccacc
     1381 accaccttgg aagaactgca cttcaaactt atgataaccc ttttgcagat acgatgagcc
     1441 ttgcatttct accataccat gaaggccatc attgctgata atgcattcat cgttaataat
     1501 taatctcgaa ccgtcatcag aactgaggcc aaagtcgtac atgccatctt tttcaatctt
     1561 gataagccct ttgtaaacaa cgccaaagtt aagctcaggg aaacctttta ttacatccat
     1621 ttcagtagct acgccctgct taacagctgt taaagtagta aaattgggca attttgtcca
     1681 agttccttca tacaggtcgt atttcaaacc ggctttaggg tttttgggct ttatagtcat
     1741 atccgagacc tgacgtttgc catcaacagt aagcattacc acagagtgcg ccggcaatgt
     1801 aacatcaacg cctgttttgg ttgttttaaa tttagtaaat gctttaatac caatttcgtt
     1861 tggtttatca aaggtattgt gggcattgag cttgtcggcc gaaataattt ctccggtgat
     1921 ttttgagaca tcataccctt tcaagttgca agtcaacact tgttctttgg tggcatgaag
     1981 gttacaaatg gtgatgtgca taacaccatt tttatcaatt gaggtggaca tattcaacga
     2041 agggacgctt ttctgattaa aagtataatc ttcgcattga agtgatgttg gaactaataa
     2101 agcatcctgg tgtactttgt acatatcaaa aacatgataa gtaggagtaa gtatcatctt
     2161 ttctttatca gtcaggataa cagcctgcaa aacattaacc acttgtgcaa tagctgcaag
     2221 ccttacccta tcgcaatggt tgttgaaaat attgaggttg ataccagcca cgagcgcatc
     2281 gcggagcgta ttttgctgat ataaaaagcc aggatttgta ccaggttcaa cagcatacca
     2341 tgtcccccat tcgtcaacag caagggctac agacttagcg gggtcgtact tatccatgat
     2401 ttcagaatgt ttggtaacta aagtctccat gcgaagggtc ttgttaagta cgtcaaacca
     2461 tccggcttcg tcaaagtcag tggcactttt ggtattagcc catgtatatg agtgcaatga
     2521 caagccccac atgttagtac ctatttcttt cattacggtt tctgtccagt tgtaatcttc
     2581 atctgaaggg cccaccatga ttttgcgaag gttgtttgaa ccatagtttt tgaggaatga
     2641 actgaaatgt aaggctaaat cagagtaata gtcaggcttc atgtttccgc cacaacccca
     2701 ggtttcgtta cctacacccc aaaacttaac gccccacgat ttgtcacggc cgttttgttt
     2761 tcggagctgg gtcatcgggc taacgttgtc agagttgagg tattctaccc attgcgacat
     2821 ttcctggata gtaccactac caacattgcc actgatatat ggttcgcacc ccagttggtt
     2881 acatagctcc ataaactcat gggtcccgaa actattgtct tcggtaacac cgccccagtt
     2941 ggtattaatc atagatggac gaaggttgcg cggcccaata ccgtctttcc agtgatattc
     3001 atctgcaaag caaccaccag gccaacgaag tgaaggtatc tgtattttct tcagggcttc
     3061 aacaacatct gtcctgatcc cgtttttatt aggtatggta gagttttcgc caacccatat
     3121 cccttcataa atacaatgtc ccaagtgttc agaaaagtgg ctgtaaatgt atttactgat
     3181 tttttgcttg gcttttgaag cgtctatttc agcaacattt ttgttggcct ggctgttgat
     3241 aacattaaca gacaagccaa aaatcaacgc gatagttacc agttttttca tactcgtgtt
     3301 tttaataagt gtaattttta caattgtagc ccaaaaataa tataataaag ttaaagaaat
     3361 gataaacaca atttgaaaaa gaacataaaa acatttttaa taatcacttt tgtcctatat
     3421 aatagtaagg ctaaagccca attaattcaa agctttaact aaccccagcc ttaaaaattg
     3481 gggttacaaa ttctaactta ctgaagcact ttagtcctga tattttaaac ggacaacagt
     3541 tttaacaaat agtaaaataa tctatttcgt tttgctaaat gtaaattcta cttcagaagt
     3601 ggtatcgcca gtaaggtatg ccattccttt gattgtaagg ttttctccat tgagagaagt
     3661 aatttgaaaa atagaagcaa gagattcgtc aatatcttcg ttatcatcaa aaatcaaata
     3721 cttacgttca ttgattttat tctttgagac tttatagtaa tattttgtgg tttttgcttc
     3781 acatgccggg gcatctcctt caatgacgtt caatatacgg ttagctgata tatcaaatat
     3841 tttatgcttt ttatatgaat caagctctga caaaatatca ttggactctt ttccatcttt
     3901 gtttaagtcc actttttcat cagaaatata tttaataagt ctccatttgc cttccaataa
     3961 aggcataatt ttgtattcgg gatgattttt aatgtacctg ctttcataat ctaaaagcca
     4021 ttttcgcact tgtttcaaat tgtttttaaa gtctttatca ttattatagc ctaaagattg
     4081 ataataatag ctttctgcat tttcaatatt gcccagccat gcatttgcaa cagccaaatt
     4141 gacacataaa ccacaataaa tttcttttgt aatacgagcc tttgggttga cagtgtcctt
     4201 ttcagttaat gcttttcccc aaacagccac acagctttca attttctttt tttgctcttc
     4261 atttaaccct ccttttatat cttctttcat tactgttttg aattgtttaa aagcatcatc
     4321 aaggtcgtca taattatgtt tccctttggc ggtataaaga tccattcgga acaacgcttt
     4381 ttgctctttg ccccaataat tgcgcaaaag agaaaatatt ctggttgaaa tgtaattttt
     4441 taaagtattg tcaatctctg tttgggatat ctgtttttcg agactttctt tcgcagctat
     4501 tgatcttgtt tcaaagaaac tatttatatt tatgggttcc ttgaaaataa caccgggcat
     4561 ttttttacct ttaacaacca attcaaacct gatagtaaaa aagaccctgt aaaaacgttc
     4621 ataatctttc ttttcagaac gttcaataac ggtatcaata gcttcttttc taactatttt
     4681 atagttaagc ctttcaaatg atatttttat atctgcttcc gattcgttat acacctgtgt
     4741 atatccttct ataggtcgtg tccaggcata ttttgggtca tttactgtat tgaaacattc
     4801 ttcatccttc cctaatcggc tggtttcaat ataaaaagtg ctaacaaacg aaggtaaggg
     4861 attgttggga tataaataat atacttccga aatcgactta tctttcaatg tctgtgagta
     4921 gattgcagtt gaacataagc atgtgcaaac cgttaagaac attagtttta tcattaatgt
     4981 tttcatccgt aaataatttt tttaataagg tcggatgaac cccgaagggc tctccgaagg
     5041 aaaactcgca tgatgaattt tcatccttgt ttgtgtttaa cgaatcatgc tcgtttcata
     5101 atgaattgtt taggttaaaa tttggttttt atagaattag taacctttca aagatattac
     5161 tattttccaa ataagccttg tttttaataa tttaccagaa ttcgaagaca aaaatgacct
     5221 ataaaattga cagaactatg tttttgattt acacaaaaat taatgtgtca caatgacaga
     5281 aatgctattt ttgcttcaat taaaaaagta taaactatga aaatcagatt aggtaaaaac
     5341 ttactgacaa tatttatgtc aggcgctgta ttgctaacac cacttttgtt tccgccggct
     5401 gcagcacaaa aaacggtatg cgccaatgaa attggcacag aaggagggta tagctacgaa
     5461 ctttggaaac aaaaaaatga tgcagaaagc tgtatgacac ttaaagacgg ttttttgttt
     5521 agttgcgaat ggaaagaaag ttctgaaaac ttcttagcac gcagggggtt caaatatgat
     5581 acaacccaga caccttcaca aattggcaat tttgttgcat cgtttgcatg caattacaac
     5641 ccttcaggca attcgtacct tgcggtttat ggttggactg tctccccgct tgtagagttc
     5701 tatatcatcg aaagctgggg tacctggaga ccaccgggag ctacatcaaa agggactatt
     5761 actgttgatg gcggcacata cgatatttat caaactaccc gtgaaaacca ggcatcaata
     5821 aaaggtactg caacttttca acaatattgg agtgtacggc gtactaaacg cacaagtggc
     5881 aatatttcta tttttaaaca ttttgaaaaa tgggaagaaa tgaatatgcc attagggaaa
     5941 ctttacgaca tcaacttgtt gatagaagga tacaaaagca gcggtaatgc tgaatttacc
     6001 agtgccacta tagacactaa cggcgacccg acagctgtta acgctattga aaacaacaca
     6061 aagcaaaact cgccattgtc tgttagtgtt gatccctctt taaatatatt ggacgtaaac
     6121 gttttaaaag catcaggcag ccccattaaa gcaaatattt atagcataag tggaacttta
     6181 ctcaaaaaag ttgagctggt tagtggtaat aacaaaattg atattaacga tcttcaaaaa
     6241 ggcttatatc ttctaagtac aagcataaat aatgaagtta ttgcccaaaa attcctgaaa
     6301 tagtatcttt actacatata ggttatgata aaaactgcca attctttgca aatgcaaact
     6361 attggcagtt ttttttacaa aaatcaacgt atttcgcgta acactttgcc attgcttaaa
     6421 aatgacaccc cttgtccact ggtcagccca atcataacag actctacaag cggctcatta
     6481 actaatgtat cagcctgcca ttcaatgata aaattagcgc ctgtaccgct tttgtcaatt
     6541 tcaggaacaa aaaagttagt tgccccaagg gggttaagaa ctgaatcttt atcaagataa
     6601 cttctgacaa actttccatc attgttgaaa tacaaaacct tggtaatttt gacagttttg
     6661 gtaaaatctg tattatgaat agctataaaa gcgctaaggt caaatatcct gttcttttcc
     6721 tgataaggga tatttgaata cacaggcaga tataacactt gtccccgtac ttttttaatg
     6781 cttgaagact cgttaatttg caaatgattt tgatcaaagc cattttgaag aatatcatgc
     6841 ccgttattac taacattcat gcatgaagaa aacaacaacc caatgcatac aaaaagtaaa
     6901 aatcggagaa ataaaaattt agaattcatg ttattggttt tttaaatttt gaagttaaaa
     6961 gtacatcaat tttcgttttg acaaatcatt taacctcttt caaaagctat attgccagaa
     7021 ataaaaaaca gactcattaa agccaggaaa tagggtaaaa atgaaattgc cacaaaaaat
     7081 gctaagtcta aaagccttga tgctgtcaac tttgcagttt tatgtttttg agttaagggt
     7141 aaattcaaaa aagctttata atcgtcatcc acgccttaaa cagatatcat gttgtcagaa
     7201 ttctatatac cctactactg gtttattcaa ccgccttctt tttcgggaaa ataatacttg
     7261 ccaaaatact aatagccaaa gtagccagaa tgatgtataa cgaataaaca tttttaaacc
     7321 caatcatttc taaataatga tgagccaaaa gttttacccc tacaaatgcc aataaaaaag
     7381 caactcccac tttgaggaaa tgaaaaaggt caacaacttt gattagcaag aagaataacg
     7441 agcgtaaccc aataatggca aatatattag aaaagaaaac gatatacggg tcgcgggtga
     7501 tactgaatat ggccgggata gagtccaccg caaaaataag gtcagtaaat tctatcatca
     7561 ccaacacaag gaacaacggg gtaaagaaaa acttaccatc tatccttttc caaaatttac
     7621 cgcctatgta ctgcggatat atgcttatat ggtttgaaag gaacttcacc agcttatgtt
     7681 ccccaacatc tattgtttct tcattgttgc ggtcgaggta cattttgata cccgaataaa
     7741 caagaaatgc accaaaaacg tacagtatcc agttgaattt gacaatcaat gctgaacctg
     7801 caaaaatgaa tatgaaacgc aaaacaatag cgccaagaac cccccaaaac aataccgttt
     7861 tataattctt tttttcgacg ctaaagccgg taaggagcat aagaataaca aatacattgt
     7921 caatagacaa ggtatattca aggaagtaac ctgcaatata atcggtagcc gcattttcac
     7981 gatatatttt taaggcttcg ttaaaatcgg tagtgttcaa ttttacatga ggcgaataga
     8041 gcctgatata gttgaccagc tcatccattg ttgtaatatt gtgaatatgg tcaccgtaaa
     8101 acctgatgaa gaaataaaac ccaatggcaa ggcaaaccca aacgcttgtc cacaacaaag
     8161 attctttgaa agaaatttca tgtttgtttt tgccaatgac caaaaggtct aataacagta
     8221 cgcttaaaat aacaatgccg aaaataaaga aaaacaagtt agaataaata aaatcaacca
     8281 tgatataaaa atttagtgca gtaaaattaa agttttattt gaaggtgttt taccattgtt
     8341 tctattccag acaaatgttt aaacacgatg ttggccaaag tgctaccggc aattccagcc
     8401 atagggccaa caacaaagct aaagcctgct gctttttcgg tatcttttat ttctgttttt
     8461 aatgaagtgt taagcattcc ggaccaaaca aaagcagaaa aacctctgca ataaacattc
     8521 aacacaattt tgttttcggg taatgcagca aatatggcat cattatcaag catattagtg
     8581 caattgagaa caatatcata gcctgcagca aacctgacaa ggttttcgca agtcagttca
     8641 atattatgaa tggtaatttg ggcgtttggg tgcaagtggg caagtttttc tttcaataca
     8701 attgttttaa gtttccccaa atcgccagtc ccaaaaatag tttgtttgta aaaatcattt
     8761 tcagaaatgg tttgataatc gcaaaggcca agcttcccta ttccggaaga caaaagataa
     8821 agcgcagcag gtatccccac cgatccaagg cctgctatta gtatttttga acttttcaaa
     8881 gccaccatac cttttaaccc aatcttcccg aaactaatgt atgaggcaaa acgtctttgc
     8941 tcgtagtcag ataatgccat attaaaatag ctttacatga agatgtaaag gtataataaa
     9001 agacaagaaa acctttatta aattaaatac aggctatctt ttcaatcaaa atatcaataa
     9061 aaaaggaatc tttattgata aatgaggtta cacccctttg ctcatattgt ttgcgtattg
     9121 cgctatcatt tgatccggta aggacaataa aacttgcccc agcgtacgtt tctctgattt
     9181 tatcaataat ttccaaacca gtgttaaaaa tgctgttatt cacaaagcta tggtctataa
     9241 caacaatgtt tgggttttta tccaaaaacg gaagacaatc ttctccttta ctaaaggcaa
     9301 aaaccttaca attgtccctg ttactaaagg tatattgcat caggttgagt atcatcttgt
     9361 catcatctat aaaaaatatc accttatcca tgcctatttg acaattgaaa ataatacaac
     9421 ccttataagc ctaataaaaa aataagcctt taactttgca ccactcgtaa agataatttt
     9481 tttttatcta aaaatcatcc ctgttatgga aaaaaatctc tggaacaaaa gtttttcact
     9541 attgtgttct gtcaactttt tggtggcatt taccttttat gctatcatgg cccttttgcc
     9601 tggttacctc aattcgcatg tcaaaataga ttcctctcaa atcgggattc tcatgtcttc
     9661 ttttacttta tcggcattat taataaggcc ttttgccgga tacctgattg acaaccaccg
     9721 taaaaaatta atattaggag ctgccatact tctgtttgcc acatcttttc tattatactt
     9781 gttttcggca acatttgtct ccatatttgt agtacgtttt atacagggca tagcatgggg
     9841 tgccttttct tctacagctt cagtgatggt tattgatgtt ttgcccgttg aaagacgagg
     9901 agaaggcatt ggcctttacg gtgtatttat gactggggca atggccatct cgccgttagt
     9961 aagtattttt gctttaaaat ttaccagcta tggcaacctt tttattgttt catttttcat
    10021 tgtatttggc tgtttaggct taattctgaa agtaaaatct ttcaatactt tttctaataa
    10081 aaaattcact tttcacgaag ttatcaatgt taaggttatc ccgctatcat tgtttatcat
    10141 gattgctacc atggcctacg gaagcgtatt gtcttttata accctttatg ccgctgaccg
    10201 acattacaac aatgtaggtt ttttcttttt ggcacaggcc attgggctaa ccctgatgag
    10261 atttctggca ggtaaagctt acgaccgcaa tggcccaaag gtattgacca tcctttcgtt
    10321 cgcctcgtta atcacaggtt ttttgcttat gatattttca ggcaacgaat tcttgttttg
    10381 cttttcggcc atatttattg gcatggggta tggctcgctg ttcccggcat cacaagccat
    10441 gattaacgaa ctggtcaccg ccaaagaccg tggaacagct aatgccacgt ttctcacttt
    10501 ttttgatacc ggggtaggct taggcatgat aagtaccgga tttttatcca aactatttgg
    10561 gtttcaaacg gcttacctta tgtgtgtagc gtatttgctc attgccctga cagtttttct
    10621 aattataggg ttaaagtatt atttgaaaca cagaataaca aatgctttgt aaaaaattaa
    10681 accattatgg gtatagatat cattctaatt gagtaatact acatttccaa tacataataa
    10741 ataagtgtaa tagatacctt tgtaatcaca caacttaact attttattat gaaacaccca
    10801 tgattctgca aatcgcaaac acgatgatat tttattgtgg gtgttctccc gccaaagacg
    10861 ggacaagcta tcattctata taaaaattaa aaaataaata tgatgaaaaa actcgcaatt
    10921 ctcattgcct taagcttttt tatcaacagc tttgggcaac agtattcaaa aaaatgggca
    10981 gacatcaact atgctggtga tgcaaaaaac taccatttaa tggacattta cctgccaaaa
    11041 gcagttaaaa cctcctatcc tgttgtggtt tacatatacg gcagtgcctg gtacagcaac
    11101 agctcaaaag gcgcagatat gaacaccatc ggaaaagctt tattagacgc aggttatgca
    11161 gtagtaaccc ccaaccaccg ctcaagtagc gatacgcttt atccgggtca aataaatgac
    11221 ataaaagctg taatacgctt tgtcagggga aatgctgcta cttatagcct cgacactgca
    11281 tttataggca tatcgggcag ttcttctggc gggcatttgg ctgcgcttgc tgcaacttct
    11341 gttggcgtta aaaaatttac tgttgattca acaaccatga acattgaagg aaatgtcgga
    11401 aaatataaca ctttcagcag taaagtacat gctgcttgcg attggtttgg cccgacagat
    11461 ttgctcaaaa tggactcatg ccgcgggagc tcaatgtatg ccgaaggtca atcacccgaa
    11521 gaaaccctta ttggaggcac aaaaaaagcc ctgagaaata aatttgtgat gctaagccca
    11581 accacctata tcgactcaac agactgccct tttcttattt tccatggcga tgctgacaat
    11641 gttgttcctt actgtgaaag tgaattgctt tacaatgccc ttcaattata tggtgtacaa
    11701 agcgaatatg taagggtgcc aggtggccag cattatacgg ggactcatac cgatgccaac
    11761 atcaaaaaaa tggttgattt ttttaataaa atttcgaaaa acaacatctc ggctgtaatt
    11821 gctttgaata acacagttaa aacttatgtt gccaacaaca cgctaaccat cgaaggtatc
    11881 tcagactttt cagtttatca atatgaaatc attgatattt cagggaaaaa gctccgctca
    11941 aatgctttta caacaagcaa aattgatatt gcatcattaa aaaacggggt ttattttttc
    12001 aaactgcaat caaaagacgg caattcaatg attgataagt ttgtaaagta aaaaaaagaa
    12061 ttattgcaaa aagcaagctg gaaatagact gcttgctttt ttcttttcat caatgtcgtt
    12121 tagttaagaa cacacccttc gactctgctc agggtgacag tcaccctgag cagagtcgaa
    12181 gggtgtgtaa tgattgaaga atcgaaaata tagccttaac taaacaacat tgaattacaa
    12241 ttgaaataat atttcaacaa aaagcctggc aaacttttac atctgccagg ctcaaaacta
    12301 acaatttacc gcagaaaacc aattaactat tttccggttt ttaccctgtt tagttcatcg
    12361 tccaagccag ttttgcgctc acgtgaaggt tttacatcat cacttccaaa acgatatgag
    12421 aagttgaatc caaactgacg gctatcccat ttatttctgt taagtaaaat caagtttcca
    12481 ttcgaccaca tttttacagt ttctttgctg gaattaaata tgtcacgaac aaaaagctta
    12541 atctgtcctc gctgtttcag tatttgtttt tgtacgccca taccaatgtt atattgacct
    12601 gatagattaa acactccgta agcctgagga gcctgatacc agctggttat ttccaaaaca
    12661 taatttttgg gaagtgtaaa gtaatggcca ctccatacat tcatggtgta actgtcgtta
    12721 ttgatattgt cactgctcct cttgctataa aatctgttgg tataagcgtt aatatttgca
    12781 ctaatgcggt accattttgt cacaggcagg tttaaccc
//
LOCUS       DAOSPF010000079        12700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4940_length_12700_cov_7.745749, whole genome shotgun sequence.
ACCESSION   DAOSPF010000079 DAOSPF010000000
VERSION     DAOSPF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_12700_cov_7.745749"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..614
                     /gene="rnpA"
                     /locus_tag="PK252_12515"
     CDS             189..614
                     /gene="rnpA"
                     /locus_tag="PK252_12515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT15575.1"
                     /translation="MANTFCKEERLYKKKEIEQVYDQSKSFLSYPLRFSYKTIKSETT
                     DPFGYKVLITASKKRYKTAVERNRIKRLCREAFRLNKDILNGVEEIHATLLIAVTCVS
                     TELPAYTQVSEQMAKGLEKIKKDIITTPTSCLRQDKLSF"
     gene            796..1035
                     /gene="yidD"
                     /locus_tag="PK252_12520"
     CDS             796..1035
                     /gene="yidD"
                     /locus_tag="PK252_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT15576.1"
                     /translation="MKILHYITKFIVFLLIIPVKIYQYTISPILPMSCRYTPSCSAYA
                     IEALKKHGPIKGLYLATKRILSCNPWGGSGYDPVP"
     gene            complement(1307..1726)
                     /locus_tag="PK252_12525"
     CDS             complement(1307..1726)
                     /locus_tag="PK252_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15577.1"
                     /translation="MKKIILFYILIFQVSYLLANDSIKYSTSLKMGPTIVPKNSELEK
                     GKYGFNAGYCFGRHFNNVLLITTINYSLCTLLEPDWFQDKYTEDVVNADLMIGYKYQE
                     SKVLIQFSAGVSYLFGNKHGKYIPPHGLFGSRGYENI"
     gene            complement(1894..2463)
                     /locus_tag="PK252_12530"
     CDS             complement(1894..2463)
                     /locus_tag="PK252_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT15578.1"
                     /translation="MKTRNLILVLFLFLFIGCQKEENTLPHTTVSGHVYSTGSGSPVE
                     GVTVYMYDGLPYSRGGSNRIDSAKTDANGYFHVELDGKEPVLDFFKENYDFEYTVGGA
                     GIGIMPVDPGSNQIDVKVQLDGIAHFSPVLMNKFPQNDEDTVILYITNSKYNIDNDIL
                     DGKGNGPHYFWDAGLKDGFFYSWRSLSEI"
     gene            complement(2516..3163)
                     /locus_tag="PK252_12535"
     CDS             complement(2516..3163)
                     /locus_tag="PK252_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15579.1"
                     /translation="MKTCLFISFLLLILYSCEPEKNSPNTIAEGYIYHSGTKKPLKGV
                     TVYIYDGLPGSSKGSNRIDSTHTDAFGFFHVELSAEEPVLDLYKENYDFEYTVEGAVI
                     GIVPLNVGNNLNLKFELDAYAYFKPYCLQGRNCNENDTVWYGFGRYINVPYAGNYLGN
                     GPHTLNTWPGDKGRLVKGDKYAYYWLKYQIKGEWHERIDSVYVPSFTTWADTIFY"
     gene            complement(3215..3868)
                     /locus_tag="PK252_12540"
     CDS             complement(3215..3868)
                     /locus_tag="PK252_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15580.1"
                     /translation="MKWISIFLVIFCLIGFGCEPEESLPHTVASGHVFSTGSGRPLAG
                     VWIYMYDGLPYSNKGSDRRDSIITDSSGYFHIELDGKEPVIFPYKVGYSFSYVVGGAT
                     IGIMPLDAGSKNENLEIRLDGEAYFNPILMNKVNFLSEDKVTVYIYSKWDNPSPPSSY
                     LGNGPHIYSWSLPDGITVLGDSYVRYKLEITKSNLMNTVIDSVYIAQGTVYRDTIWY"
     gene            complement(3875..6715)
                     /locus_tag="PK252_12545"
     CDS             complement(3875..6715)
                     /locus_tag="PK252_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15581.1"
                     /translation="MQAQEEETYSDEDTASVITKDVYKQNPGWYLRNIDSAKIPTGIL
                     IDRVKFKNDMGLFNGKSRVKTCDYAVFRRVVKHLQKAANDSVYFPNLDSVYEFAYHKM
                     RWEQIYPIAIMDYSYNKVRKKAFLSKLAELTDTAMTLTSGYDSIFATRRCFLASPFNI
                     RMHGDNIQVAFYRNMFFTNSNDSVIKIEANFGNGWKTFDWDEPQSISFSAKTQYVEAK
                     VRLTYVKNGENKQRKVISHFTVLHSGSDLVPQASDVKTSKLKSTSVEPSYKIFFPSSK
                     VITAGYHCLMTINGLCQKWQTLYDYSNCDIEASILFAPSNKTGKLRKPIIVCDGFDPG
                     DKRDFIQTYIEKLDEQLTYSKDTRGIYELVNGDPSPWYNSEEFPEYQSKSAGLVSTLQ
                     ASNYDLVIVNFLKGAGDIRTNAYWFRRFLKEVINSGSLRDNQTEENVIIGPSMGGIIT
                     RIALDSAEILGDEPLVREWFSFDSPQEGAYIPLSLQWAIHYLDDLSNHENGSLLASLG
                     ALNSPAAKQMLLLHYQSMVAQGHTGTPNPLYNFYQLYNTLHSLKYPKLSRNIAISNGG
                     NTNLYSDNIKEIAYLRAVGASALFGAIEWDVRIWLNNNNNVNNNSYCALFKGEVDDYN
                     SDDDYTLYTNYQIGYENSPGGWHSALYDFNMNADNENKPDIIANTQNRWATFMPTCSA
                     FGIVPDQYNVHRTGSDLKVTYKSQIPFDDTYSMAGANEEHVRISKSTGEKVTQYLNEW
                     DLDTIQRPRLRPNQNNSQSISGSVKIKATNWIKLGSKNGKNNIAFNSGADVNLSATNS
                     VKFLPGAKAVKGAKIKVKVGASVSKKSELAQVQPKPVSYPITSPFVGKVFSYDDNQEK
                     PTEINIFKQLDKNVSIFPNPSGGTINIVVTGENVIEGMFIVTNVFGNKVMSNKVNSEK
                     EILDLTNLANGIYFVAVSINGVSYNEKIILNK"
     gene            complement(6810..7499)
                     /locus_tag="PK252_12550"
     CDS             complement(6810..7499)
                     /locus_tag="PK252_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15582.1"
                     /translation="MDFGFRISDFGLLTLCIIVFSAFSAFAGAQTCSSPFVCGSNPNC
                     TGGQQADLKLNGQGGLQTFATFSYCDGIKFFKNLSVNATITATNFVGNGANLTNAAGW
                     VKSGSNIFTSGSVAIGCSSFPSGVMLAVNGKIQASEFEIKTTPCSDFVFEKGYKLRSL
                     KEVEQFVTENMHLPEIPSAKEFKEAGSYKVAEMDDLLLRKVEELTLYTIELNKQVEAL
                     KAELAKVKGGE"
     gene            8343..8477
                     /locus_tag="PK252_12555"
     CDS             8343..8477
                     /locus_tag="PK252_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15583.1"
                     /translation="MKTEENKSVSVMAAYSFEEEDDFYFLDLESINKNHALLQHIILN
                     "
     gene            complement(8479..9303)
                     /locus_tag="PK252_12560"
     CDS             complement(8479..9303)
                     /locus_tag="PK252_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT15584.1"
                     /translation="MGYINVNSDKCTKCNTCIASCPVACISSDSASKLPVVKTEKEAL
                     CVYCGHCESICPEGALVHSLSQQALQSAKEQSKLVNAADLALYFKQRRSIRNFKKEVV
                     DKQLLEQAFDVVRFAPTGHNRQKNQWIVVYNTKVVEAIAAATIDWMRGVVKANPEIGT
                     RYGFPGLVIAFDRGRNLISRNAPHLIICYTPASHPVGPKDAVIATTQMDLYLPSLGVG
                     TCWAGFIYMALQQSDELKKIVGLDENSAVHAALLTGYPKYNYHKIPHRKAADVRWI"
     gene            complement(9621..10244)
                     /locus_tag="PK252_12565"
     CDS             complement(9621..10244)
                     /locus_tag="PK252_12565"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOT15585.1"
                     /translation="MKNINFCILMSIFGLFSCENDVTIHVGDTKNIVGNGELIEHRLD
                     VEKFSKVSVTGQAKLDVVYGDTFMVKVRAQQNIYEMLDLKVINGNFTIGVKENKSIST
                     NKGIYIDIVTPSAIEDVIITGAGTVSFSKGKQQKLNISIAGTATINAFNLEAENVKVT
                     IAGSAQCEVLATKKLDVSISGTGTVKYKGRPSISQSIAGLGSVVSEN"
     gene            complement(10398..11711)
                     /locus_tag="PK252_12570"
     CDS             complement(10398..11711)
                     /locus_tag="PK252_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT15586.1"
                     /translation="MLVKKYQTATAEEAVKIVKSGDHVHFSSVASAPQSLINALIERG
                     RRGELRDVTIHHLHTEGPAPYAAKEFEGIFQHDAFFVGANVRAAVQSGYADYIPVFLG
                     ETQRLYREGYLPCDVCMLQVCPPDEHGFVSMGTSVDATLAAVENAKTVIAVINPNVPR
                     SFGDALIHMDMIDIFVEDGTPLEPGHFSAPDEIETAIGKNCAALIEDGACLQMGIGSI
                     PNAVLSQLGNHKNLGIHTEMFADGVLPLVEKGVINGSNKTTDMGKIVATFLLGSQKVY
                     DFIDNNPEVLMMDVGYTNDPFIISKNEKVTAINSALQIDLTGQVCADSIGTKHYSGVG
                     GQIDFVYGASRSKGGKAIIAMPSVTNKGVSKIAPTLLNGAGVVTSRFHVHWVVTEYGA
                     VNLYGKSMQQRAKLLTSIAHPDHREELDKAAFERFGPHFHYIKKH"
     gene            complement(12045..12572)
                     /locus_tag="PK252_12575"
     CDS             complement(12045..12572)
                     /locus_tag="PK252_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15587.1"
                     /translation="MKIKIKGILIALIIAFFGVGLKAMGSDTSFVNQKITLGQDTNCN
                     KKNPYLSCILSAVLPGTGQLYNGQYDKGIAMLIFEIGGGAMTIYGLEHIIKSGDIFLG
                     GLLILSVNTIWSAIDAPKSSIKINEKNGLCYELNNKMHIGLKPNLFSFRQYNKVSRVE
                     LIFSIDLNRLWPLLS"
BASE COUNT         4063 a         2552 c         2027 g         4058 t
ORIGIN      
        1 acggcaagcc ttgaattttt gtaacttttg cttcaaggca aaagttagga atgcctctaa
       61 atatcgaata agtaagcttt tttattatat tgaactaaca tagattctaa cattttgtac
      121 tgcctcgttt tccagcttct tcgacccata aacttatttc cattatcttt gttcatcaaa
      181 tatcattcat ggccaatact ttttgcaagg aagaacggtt gtataaaaaa aaggaaattg
      241 agcaggttta tgaccagtca aaatcgtttt tgtcttatcc gctccgtttt tcttataaaa
      301 caattaaaag tgagacaacc gatcccttcg ggtataaagt gctaattacg gcctctaaaa
      361 aacgctataa aacggcagtt gaacgtaacc gtatcaaacg actttgccgc gaagcatttc
      421 gtttgaacaa agatatactc aatggcgttg aagaaataca tgctacactg ctcattgctg
      481 ttacttgtgt ttcgactgaa ttgcctgcat atacccaagt ttctgaacaa atggccaaag
      541 gattggaaaa gattaaaaaa gatatcataa ccacccctac ctcctgcctg aggcaggaca
      601 agctctcctt ttaaaggagg gaaacacctc aataatttat aattcaatgt ttagtcaata
      661 caatattttt tataatcaca tcacaaaaga cccttcgact ccgctcaggg tgactgttat
      721 cctgagtccc gcttaaaaca ggaaaggatg ttttctaaat ttaatggcat agatttgtaa
      781 tttttatttg aaaaaatgaa aatactgcat tacataacaa agtttattgt tttcttgctg
      841 attataccag ttaagattta tcaatatacg atatcgccta tattgccaat gtcttgccga
      901 tatacaccaa gctgttcggc ttatgccatt gaagcattga agaaacacgg gccaattaaa
      961 ggtctttacc tggctactaa gcgtatttta tcctgcaacc cttggggggg tagtggatac
     1021 gacccggttc cgtgacaatt aaaaatgaaa aattaagaat tgataaaaaa acgccttgta
     1081 tttttgaaat gcaaagcgtt ttttactatt gtatcctttt aaaatcaatt gcccaacgta
     1141 atcctatata aaactaacta tatcataaat ttacagtaca aattaaatat ggactatgat
     1201 aagtatagtt tttattagta tttagataat atcccaaccc caacaatact tcatctctaa
     1261 taaaataagc catgctaaaa tctataggcc atccatagcc actgaatcag atattttcat
     1321 accctctact tccaaataaa ccatgtggag ggatatattt accatgttta ttaccaaata
     1381 agtatgatac gccagcacta aactgtatca aaaccttact ttcctgatac ttatatccaa
     1441 tcattaaatc agcattaaca acatcttcag tatatttatc ctgaaaccaa tctggttcaa
     1501 gtaatgtaca taacgaataa tttattgtgg taattaacag aacattatta aaatgtcttc
     1561 caaagcaata gccggcattg aatccatatt ttcctttttc aagttcacta tttttgggta
     1621 ctattgtagg ccccattttt aaagatgttg aatacttaat gctatcatta gctaaaagat
     1681 agcttacttg aaatatcaaa atgtaaaaca atataatttt cttcatttta caacttttgc
     1741 agaatttata ttctaataat ttttaaaaat aagcagggct gttacaccca gcttatctat
     1801 tagtaccata taatatccct gtacaccgta cctaccgcaa catagacact atcaacgttt
     1861 gcattccatt gattattcct ttcatattta actttatatt tctgataatg atcgccaaga
     1921 atagaaaaaa ccgtctttta atccagcatc ccaaaaataa tgaggaccgt taccttttcc
     1981 atctaatata tcattatcta tattgtattt actatttgta atatacaata ttactgtatc
     2041 ttcatcattt tgaggaaatt tattcatcaa aacagggcta aaatgagcta taccgtctaa
     2101 ttggactttc acatctatct gattcgagcc agggtcaact ggcataatgc caataccagc
     2161 acctcctaca gtatactcga aatcgtaatt ctccttgaaa aagtctaaaa caggctcttt
     2221 gccatccagt tctacatgaa agtagccatt agcatcagtt ttagctgagt caatcctatt
     2281 gctcccacct ctgctatatg gcaggccatc gtacatataa actgttacgc cttctacagg
     2341 acttcctgaa cctgtgctat acacatgccc actaacggta gtatgtggca aagtgttttc
     2401 ttctttttgg cacccaataa acaagaaaag aaaaagtact aaaattaaat ttctggtttt
     2461 cattctgttt tttattaaac aggttggagc ataaaaatcc caacctgttt taattttagt
     2521 aaaaaatagt atctgcccat gttgtaaaag aaggtacata tacgctatct atcctttcgt
     2581 gccattcgcc tttgatttga tacttaagcc aatagtatgc atatttatcc ccttttacga
     2641 gtcgtccttt atctccgggc catgtattta aggtatgtgg cccgttccct aaataatttc
     2701 ccgcataagg aacattgata taccttccaa aaccatacca aacagtgtca ttttcattac
     2761 agtttcgtcc ttgaagacaa tatggcttaa aatatgcata agcatctaat tcaaacttta
     2821 agttcagatt atttccaaca ttcaaaggca caattccaat aactgcacct tcaactgtat
     2881 attcaaaatc ataattttct ttatacaggt ctaaaacagg ctcttctgca cttaattcta
     2941 catgaaagaa tccaaatgca tcggtatgag tagaatctat ccggttactt cctttgctgc
     3001 taccgggcaa gccatcataa atataaactg ttaccccttt gagtggtttc tttgtgccag
     3061 agtgatatat ataaccctct gcaatagtat ttggagagtt cttttcaggc tcgcaactat
     3121 ataatattag tagtaaaaag ctaataaata aacaagtttt catttctgtt tcagttgtaa
     3181 taaacaagcg gggctttaca accccgctta tattctaata ccatatagta tccctataaa
     3241 cagtaccctg cgctatatat acactatcta taaccgtatt catcaaattg ctttttgtta
     3301 tttcaagttt ataccttaca taggaatcac ctaatacagt aataccatca ggtaatgacc
     3361 acgaataaat atgtggcccg tttcccaaat agcttgaagg aggagatgga ttatcccatt
     3421 ttgaatatat atatactgtt actttatctt cagaaagaaa attcacttta ttcattaaaa
     3481 ttggattaaa ataggcttcc ccatcaagac gaatttctaa attctcattt tttgaacctg
     3541 catcaagagg cataatacca attgtagcac ctcccactac gtaggaaaat gaatagccta
     3601 ccttataagg gaatattaca ggctccttgc catcaagttc aatatgaaaa tacccacttg
     3661 aatcagttat tatagagtcc ctccggtcac tgcctttatt gctataaggt aaaccatcat
     3721 acatatatat ccatacacca gctagaggtc tgcctgaacc tgtgctaaat acatgcccac
     3781 tggcaacagt atgtggaaga gactcttctg gttcacagcc aaaccctatt aagcaaaaaa
     3841 taacaagaaa aattgaaatc catttcatac aaatctattt attaagaatt atcttttcat
     3901 tataactaac cccattaatg ctaacagcaa caaaataaat accattggcc aaatttgtca
     3961 aatcaagtat ttctttttct gaatttacct tatttgacat tactttattg ccaaaaacat
     4021 tcgttacaat aaacatacct tcaataacat tttcgccagt tacaacaata tttatagttc
     4081 caccagatgg attaggaaaa atgcttacat ttttgtctaa ctgcttgaat atatttattt
     4141 cagttggctt ttcctgatta tcatcatacg aaaatacttt accaacaaaa ggagaagtaa
     4201 taggatagga tactggtttg ggctgtactt gggcaagttc agacttttta gaaacagatg
     4261 ccccaacttt tacctttatt tttgcacctt ttacagcctt agcacccggc aagaacttga
     4321 cactgttagt cgcacttaaa tttacgtcag caccagagtt aaaagcaatg ttgtttttgc
     4381 cgtttttact gcccagcttt atccaattgg ttgccttaat tttcacagag cctgaaattg
     4441 attgagaatt gttttggttg ggtctgagcc ttggccgttg aatagtatct aaatcccatt
     4501 catttaaata ctgagttact ttttcccccg tacttttact aatcctcaca tgctcctcgt
     4561 ttgcaccagc catgctgtaa gtatcgtcaa aagggatctg gcttttgtag gttactttaa
     4621 ggtcgctccc tgtgcgatgt acattgtatt ggtcgggcac aattccaaag gcagagcatg
     4681 taggcataaa agtagcccac ctgttttgtg tattagcaat aatatcaggt ttgttttcgt
     4741 tatcagcatt catattaaaa tcatacaatg ccgaatgcca accgccaggt gaattttcat
     4801 aacctatttg ataattggta tagagagtat aatcatcatc tgagttataa tcatctactt
     4861 ctcctttaaa aagagcgcaa tatgaattgt tattgacatt gttattattg tttagccaaa
     4921 tacgcacgtc ccattcaatt gcaccaaaca aagcagaagc ccccacagcc ctgaggtaag
     4981 caatttcttt aatattatct gaatacaagt tagtatttcc accattagag atagctatat
     5041 tccgcgataa ttttggatat tttaagctgt gtaaagtgtt gtacaattga tagaaattgt
     5101 acaatgggtt tggagtacca gtatggcctt gtgctaccat tgattgataa tgcagcaata
     5161 acatttgctt tgcagcaggg ctgtttaatg cacctaagct agctagcaaa cttccatttt
     5221 catgatttga gaggtcgtca aggtaatgta tagcccattg caaacttaaa ggtatgtagg
     5281 ctccttcttg tggagaatca aacgaaaacc attcccgaac aagaggttca tcacccaata
     5341 tttcagcaga atccaaagca atacgggtaa ttattcctcc catgctgggg ccaataataa
     5401 cattttcttc cgtttggtta tcccttaatg atccactatt aatcacttct ttcaagaatc
     5461 gtctaaacca atatgcatta gtccttatat caccggcccc tttcaaaaag ttgacgataa
     5521 ccaaatcgta attacttgct tgtagagtac ttacaagtcc ggcacttttg ctttgatatt
     5581 caggaaattc ttcactattg taccatggtg aagggtcacc atttacaagc tcataaatac
     5641 cccgagtatc ctttgaataa gtaagctgct catcaagttt ctcaatatag gtctgaataa
     5701 aatccctctt atctcccgga tcaaaaccat cacaaacaat aataggcttt cgcaattttc
     5761 cggttttatt tgaaggggca aacaaaatgc tagcttcaat atcacaattt gaataatcat
     5821 ataatgtttg ccatttttga cacaacccat taattgtcat caaacagtga tatcctgctg
     5881 ttattacttt agaacttgga aaaaaaattt tataacttgg ctcaacagat gtgcttttta
     5941 atttactggt tttaacatca gaagcctgag gcacgaggtc tgagccacta tgcaagactg
     6001 taaaatgcga aataaccttt ctttgtttgt tttcaccatt tttcacatag gtcagcctta
     6061 ccttagcttc aacatattgt gtttttgcac taaaactaat ggattgaggt tcgtcccagt
     6121 caaaagtttt ccaaccattg ccaaagttcg cttctatttt tataacagaa tcattagaat
     6181 tagtgaaaaa catattcctg taaaaagcaa cctgaatatt gtcaccatgc atacgaatat
     6241 taaaagggct tgccaaaaag caacgacggg tggcaaaaat gctgtcatat ccggaggtca
     6301 aggtcatagc agtatcagtt aattcagcta atttactcaa aaaagctttt ttacgtacct
     6361 tattgtatga ataatccatg atggcaattg gatatatttg ttcccatcgc attttatggt
     6421 acgcaaattc atacacacta tcaaggttag gaaaataaac tgaatcattg gcagcttttt
     6481 gcaaatgctt aacaactcgt ctaaaaactg cataatcaca agtcttcacc ctgcttttgc
     6541 cattgaataa acccatatcg tttttaaact tcacacgatc aatcaatatc cctgtcggaa
     6601 tttttgcact atctatattt cgcaggtacc agccggggtt ttgtttatac acatctttag
     6661 taatcacact tgccgtatct tcatcgctgt aggtttcttc ttcctgggct tgcatcttta
     6721 tgatgcacaa aactaatatg caaagtacaa gcagtattgt ctttgctccc ctttccttcg
     6781 gaaaggggtt gggggatagg catttcatct tactcccctc cctttacctt tgccaattca
     6841 gctttaagag cctcaacctg cttgttgagc tcaatggtat aaagagtaag ctcctctact
     6901 ttacgcagta gcaggtcgtc catctcggcc actttgtagc tcccagcttc tttaaattct
     6961 ttagccgaag gtatctcggg aagatgcatg ttttctgtaa caaattgttc tacttctttt
     7021 aaagaacgta atttatatcc cttttcaaat acaaaatcag aacaaggcgt agtttttatt
     7081 tcaaattcac tggcctggat tttcccattc acagcaagca tcactccgct tggaaaactt
     7141 gaacacccaa tagccacgct gccacttgta aaaatattag aaccactttt tacccaaccg
     7201 gctgcattgg ttaggttagc gccattgcca acaaaattag tagctgtaat agtagcattt
     7261 acagaaagat ttttgaaaaa tttaattccg tcacaataac tgaatgttgc aaaagtctgc
     7321 aaaccacctt gcccgtttag ttttaagtct gcttgctgcc ctccagtaca attagggttt
     7381 gaaccacaaa caaatggaga gctgcaagtt tgcgcccctg caaatgccga aaaagcacta
     7441 aatactataa tacataaggt taataacccg aaatccgaaa tccgaaatcc gaaatccaaa
     7501 atcttgtaac atttttttgg taattataaa atggttttta aaggttaata aaaacattat
     7561 caagtattaa tgtcactaaa gataaaaaag taaccctgtt aataggtaat ttttaagcaa
     7621 aaatgctaag cgtttttgaa aaacgcttag catttgaccc caatcaagaa ccagacagat
     7681 ttaaaataat aactatttta tcatctttta gacttttaat cgtatattat atttaatttt
     7741 aacaaattat aaactttatt ttatattcat acttttaacc tgtaaagtta attcattgtc
     7801 aaactgttgt ttatatataa tttttcgttt atagcaaaca gggatgtgtt gacgcaacag
     7861 cataaaaacc tttgtccaca attttcatat aacaaattga cagactttga tatatactaa
     7921 tgtatatcaa caaattgttt tatgaagtga aatatttcat tggggggtaa tgtcattatc
     7981 tattgtcttt ttaaaagaca agttttgttt acagacaatt aatactcatg taataatttg
     8041 ataatatgaa cgttctatgt ttgttgcaga tttaacggga tgaaataccc atgatttatt
     8101 aagatgatgc gggtgttctc ccgccaaaga cgggacaagc tatcattcca ttaaaaatta
     8161 aaaaagaaat atgatgaaaa aagaaaccaa aaagaacagg caaaggaatc ttttcaaaaa
     8221 tgaaaaccaa gactcccatt tattagaaaa agatttttac aaaaagttca atctcatttt
     8281 ccaggaacta aacctcaatt ggcattatta atcacgagca tgatcgttaa tcacaaacaa
     8341 ggatgaaaac agaagaaaac aaaagtgtaa gtgtaatggc agcctattca tttgaagaag
     8401 aagatgattt ttattttctt gatttagaaa gcataaataa aaaccacgct ttgcttcaac
     8461 acatcatcct gaattaggtt atatccacct tacatctgcc gctttgcggt gagggatttt
     8521 gtggtagttg tatttcggat atcctgtcaa caaagcagca tgtacagcag agttttcgtc
     8581 cagtccaaca atttttttca actcgtccga ttgctgaagt gccatataga taaacccggc
     8641 ccagcaagtg ccaactccca atgatggcag ataaaggtcc atttgagttg tagctataac
     8701 agcatcttta ggccctacag gatgtgaagc gggcgtataa cagataatca aatgtggggc
     8761 attgcggcta ataaggtttc gaccacggtc aaaagcaata accaatcctg gaaatccgta
     8821 gcgggtgcct atttctggat tggcttttac aactccccgc atccaatcaa tggtagcagc
     8881 agcaatagct tcaacgactt ttgtattata cacaacaatc cactggtttt tttgtcggtt
     8941 atggcctgtc ggggcgaaac gcaccacgtc aaaagcctgt tcgagcaatt gcttatctac
     9001 aacctccttc ttaaaattac gaatagagcg gcgttgttta aaataaagag ccaaatcagc
     9061 tgcattaaca agtttactct gttcttttgc actttgtaaa gcctgctgag aaagagaatg
     9121 cacgagtgcc ccttcaggac atatcgattc gcaatggccg caataaacac acaaagcttc
     9181 tttttctgtc tttacaacag gtagcttact tgcgctatca cttgaaatac acgctacagg
     9241 gcaagatgca atacaagtat tgcatttggt gcatttgtca gagtttacat tgatgtatcc
     9301 catttttaaa tttaaaaaaa atgccaacta tataacattt gcagatattc gtaactttta
     9361 atcttaagca aaggtattaa aaaatgaagg cttactgaaa ttgggcaaaa aagatttatg
     9421 tccgcttgca acaacccgct ttgtccaaac agtggccttc gcttcgccgg gattaaaccc
     9481 taattaatta taagaggctt tttaaagccg aagtatatga ttaagtcagg tcaatacgag
     9541 acacacggat taagcaattt taacgcctaa tttgggttta tacaaaagag caacaacgtc
     9601 ttgcatgata tatcgtttat ttaattttca cttaccacac tacccaaacc ggctattgac
     9661 tggcttatag acggccggcc tttgtatttt acggtgccag tgccgctaat agaaacatcg
     9721 agttttttcg tggccagcac ctcacattgg gccgaaccgg caatcgtcac ttttacattt
     9781 tcagcttcga ggttaaaagc gttgatagta gccgtcccgg caatggatat attgagcttt
     9841 tgttgtttgc cttttgaaaa agaaacagtt ccggctccgg taattattac atcttcaata
     9901 gctgatggcg ttacaatatc aatatatata cctttgttgg tgctaatgct tttgttttcc
     9961 tttaccccta tggtaaaatt gccgtttata accttcaaat ccagcatttc ataaatgttt
    10021 tgctgtgccc tgacttttac cataaaagta tcgccataca ctacatccaa tttagcctgc
    10081 ccggtaactg agacttttga gaacttttct acatcaagcc ggtgctcaat caattcgcca
    10141 ttacctacaa tatttttggt atcaccaaca tggattgtca catcattttc gcacgaaaaa
    10201 aggccgaaaa tcgacatcaa aatacaaaag tttatgtttt tcatttttta aaaattttgt
    10261 tgaaaaattg gtttcttttc atcaaaacaa aaattgagga caaaacccct aaaagatttg
    10321 aaaaaaaaat tgactatact tttggacata aaaaaaccct ggcaaattgc caaggctttt
    10381 catatattcc atattttcta atgtttcttg atataatgaa agtgtgggcc aaacctttca
    10441 aatgcagctt tatcaagctc ttctctatgg tcggggtgtg caatgctggt aagcagtttg
    10501 gcacgttgtt gcattgattt gccataaagg tttacagcac catattcagt tactacccaa
    10561 tgcacatgga aacgtgaagt aaccacgccg gcgccattta ataaagttgg ggcaattttg
    10621 cttacacctt tgttagtaac agaaggcatg gcaatgatgg ctttaccacc ttttgagcgt
    10681 gaagcaccgt aaacaaagtc tatttggcca ccaacacctg agtaatgctt tgtaccaata
    10741 gaatcggcac aaacctgacc tgtcaaatca atttgcaatg ccgagttgat agcagtaact
    10801 ttttcatttt tagaaatgat aaaagggtcg ttggtataac ctacgtccat catcagcact
    10861 tcggggttgt tatcgataaa atcgtaaact ttttgtgaac ctaaaaggaa ggttgcaacg
    10921 attttaccca tatctgtagt cttgttggag ccattgatga cacctttttc caccaatggt
    10981 aaaactccat cggcaaacat ttcggtatgt atcccgagat ttttgtggtt accaagctga
    11041 gacaacacgg cattaggaat agaaccaata cccatttgaa ggcaagcgcc atcttcgata
    11101 agggcagcac agttttttcc aatggcggtt tctatttcat cgggtgctga aaaatgaccc
    11161 ggctcaagag gcgtaccatc ttcaacaaag atatctatca tatccatgtg aataagtgca
    11221 tcaccaaaag aacgtggtac attagggttg ataactgcaa taacagtttt ggcattttca
    11281 actgcagcaa gggtagcatc tacagaagtc cccatagaaa caaacccgtg ttcatcagga
    11341 ggacaaacct gcaacataca aacatcgcat ggcaagtaac cttcgcgata caaacgctgt
    11401 gtttcgccca aaaatactgg aatataatcg gcatagccag attgtacagc agcccttacg
    11461 ttggcaccga caaaaaaggc atcatgctgg aaaatccctt caaattcttt agcagcataa
    11521 ggcgcagggc cttcggtatg taaatggtgt atggtaacat cacgcaattc gcctctcctt
    11581 ccacgctcaa tcaaagcatt gataaggctt tgaggagcag aagccacact gctgaaatgc
    11641 acatggtcac ctgattttac aattttcaca gcttcttcag ccgtagcggt ttgatatttc
    11701 ttgactaaca ttgtttttta atttgagcgg caaaaataat caatttattc ataagctatt
    11761 gcgtattaac aatattttta ctcgccccta ttgtgttgtt tatctattgg ttacacaaca
    11821 atcgtaaata tttatgcaga taaaaattat aaatatcctt gaggcttgaa aataatcctg
    11881 ctttaacaaa ttgaaaagca acgtagttgc ggtttctttg cagaaagata acagacctgt
    11941 aattaattgt ttcatctttt aattttttta tacaggctac agcctgtttt gataggttcg
    12001 acggaggcat agccatcctc cgaactttcc tattttttac agcctcagct taacaggggc
    12061 catagccgat tcaaatcaat agaaaatatc aattctactc ttgaaacttt attatattgc
    12121 ctaaatgaaa ataaattagg cttaagccca atatgcattt tattgtttaa ttcataacat
    12181 aacccatttt tttcattaat ttttattgag ctttttgggg catcaatagc agaccatatt
    12241 gtattaacgc ttagaattaa taacccgccc aaaaaaatat ctccactttt aataatatgt
    12301 tctaaaccgt aaatagtcat tgctccaccg ccaatctcaa atataagcat agcaatacct
    12361 ttatcatatt gtccattata caattggcca gtcccaggca atacagcaga aaggatacag
    12421 gacaaataag ggttcttctt gttacaattt gtgtcctgac ctaaagttat tttttgatta
    12481 acaaaagaag tatctgaacc catggctttg agcccaacac cgaaaaaagc aattattaat
    12541 gctattaaaa ttccttttat ttttattttc attttgctat tgttttatgc aggaacccca
    12601 tgcctgaagt tcttgctaaa ttaaacttag taataaatag tatcagtaaa agtctcaaac
    12661 gattttacat acacactatc tatcttttct ttccattgac
//
LOCUS       DAOSPF010000080        12605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4984_length_12605_cov_6.159522, whole genome shotgun sequence.
ACCESSION   DAOSPF010000080 DAOSPF010000000
VERSION     DAOSPF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_12605_cov_6.159522"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PK252_12580"
     CDS             complement(<1..243)
                     /locus_tag="PK252_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15588.1"
                     /translation="MNTKFGISLIIAGTLVLFSCKKSDLASTGDTSETTGWAYNSEEN
                     GGFEVVTQYEEQTPPGCVFIEGGTFTMGRVEQDVNFD"
     gene            complement(311..1345)
                     /locus_tag="PK252_12585"
     CDS             complement(311..1345)
                     /locus_tag="PK252_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOT15589.1"
                     /translation="MSIKTLFLSKRIYTSNYCINWMRQIFLKQILIGCISMFYINVVA
                     QDVSFSHYFLNSVVENPAVTGSVNDGRVFLHYRNQLPAFGSTYVTYQGSYDKFSPKMK
                     SGWGLNILRDNIAGNVITNTQADILYSYRFKESHNMKMQLGLQASFQFYNINTSSLNV
                     ADPNLPADFSSTQPDFGVGYLAMTRYSQFGISVGHLNRSFLLLNNTFVESPMKFSVFY
                     SHDIELTNYRKTVAPVYTLTPALMVLYQGGSMMFDYGASLKFNNIIAGLWCRTNVPIQ
                     VTNAIASIGFIIDNWQIGYSYDYNVLSLKNIMPYTGAHEIAIVARFKQDPKRKRFGSI
                     PCPDVIIPEK"
     gene            1401..4808
                     /gene="porU"
                     /locus_tag="PK252_12590"
     CDS             1401..4808
                     /gene="porU"
                     /locus_tag="PK252_12590"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOT15590.1"
                     /translation="MKKSLYITLLLLPQIVFGTDISLSKYSATTENSVLSSGTWYKMK
                     ITNDGIYKLTYSQLKKWFSKPSNVKVYGGESKMLSKNANETKINDLQQVALYINKGSD
                     NTFNEGDYILFYGEGPDKWSFDSTRNIFRHTKHQFSDYNYYFITDNEGAQNIIQEANN
                     GSNSSNNISITTFDDYSFIEHNDVNLINSGAEWYGDLISNGSKKTFSFNFTNIVSLSK
                     AYVYYDLVSNASTEATFTLKYGNDTKTFVVTAPFSSETVGNKKTGGYYIDITNSAINI
                     SVGYTNSASSEGRGFINYLGINVIRELTFDGSQLKFRTKESANNPVLKYFIKNGTSNT
                     LVWDVTDAKKPKTISTNLTDNNIVFSDSGNKIKQYIAFDNSSGFLTPEVIEKPIANQN
                     IHGANTPDMIIVIPQDSAFYLQANRLAAFRKQNDNINCMVVDVQQVYNEFSSGKKDVT
                     AIRDMVKMFYDRGNADGNHLKYLLLFGKGTFANHIEKENNFNLIPVYQSQESLNKGGS
                     YATDDYYGWMNTQSSESANLLQIGIGRLPAKNGKEAKALIDKIIAYDNPSNISDWQNT
                     ISFIADDIDESWEHSFEANSDKYAQYIETMFSRFTIQKIYLDAFKQITTSIGESYPEA
                     KLMFNNRINNGALVVNYVGHGNEKQLAGENIIDISSIRSWNNKSKLPLFITATCEFSR
                     FDDVNLAESKQITSAGEEIILNQYGGGIALMTTTRQVYNDDNNILNDAILKNLFLKDE
                     NGNKLRIGDVFMKAKNRASSTGLNRLNFTLLGDPSLALKYPEERIIAIDSVNGKNNFT
                     DTINALDLVNIKGHLEPQEGFSGNLYITIFDKEDSIETLLNDEASLPFQYATWQKIIF
                     RGEASIANDSFSFTFPVPKDIKYQVGKGRMVFFATDGKQYFSYDNKNIIVGGINNNVA
                     KNYEGPTIQLFMNDTNFVSGGITDSQPALLAHIKDENGINSTGNGIGHDITAVLDGDL
                     LNTFVLNDYYRNSIDSYQEGTVYYPFSEIETGKHQIKLMVWDIFNNYSEANIDFEVVN
                     SESPTLKKIYNYPNPMRNSTSFVLEHNWPDKNLKVEIMIYSMTGERVASLKYQGSFAG
                     YKTAPINWDGTNQNGTKVFPGIYIYKAVITDTDNQQSSNFGKLVIVR"
     gene            4852..>5168
                     /locus_tag="PK252_12595"
     CDS             4852..>5168
                     /locus_tag="PK252_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15591.1"
                     /translation="MIRKTILVSVILFSGICNTITAQTQLGQSFQEDYNIIQTPVPFI
                     NIAPDARSSGMGDLGAATTPDLNSLHWNASKYAFLEDKYGLSTTYTPWLRGLSNDIKL
                     LYLT"
     assembly_gap    5169..5268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5269..5876
                     /gene="porV"
                     /locus_tag="PK252_12600"
     CDS             <5269..5876
                     /gene="porV"
                     /locus_tag="PK252_12600"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOT15592.1"
                     /translation="DISTYYHTPIRMGSNDGEWAMGINISNIGSKISYNDGDVKSFIP
                     TNLRLGTRFTYDIDQYNSFSAGMDVNKLLVPTPPIYSDSDRVTIAKGMNPDVSVAQGI
                     FQSFYDAPGGMSEELKEVMLSIGAEYWYQKQFALRAGYFNEAAMKGNRKYMTVGVGLK
                     LTTITIDFAYLIPNGGRSNPLANTMRISASFDIGHPKKTKK"
     gene            5873..6352
                     /gene="ispF"
                     /locus_tag="PK252_12605"
     CDS             5873..6352
                     /gene="ispF"
                     /locus_tag="PK252_12605"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT15593.1"
                     /translation="MMFRIGFGYDVHQLVEGRGFWLGGIEIPHTKGSLGHSDGDALIH
                     AICDAILGAANMRDIGYHFPDTSSDFKNIDSKILLKRTIDLARQKGFEIGNIDSTICL
                     QKPKVKDYIPVMQQTLAEVLSISTNDISVKATTTEKMGFVGREEGVAVYAVVLLEKK"
     gene            6506..7756
                     /locus_tag="PK252_12610"
     CDS             6506..7756
                     /locus_tag="PK252_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOT15594.1"
                     /translation="MVDIFDKIEKNRGPLGQYQKEAEGYFMFPKLVGEIGPYMKFKGK
                     EVLNWSLNNYLGLANHPEVRKADAEGAAQYGLAAPMGARMMSGQTDLHEELEARLAKF
                     TGREAAYLANFGYQAMISILDTILNRNDVVVYDSEAHACIIDGLRLHLGKRFVYPHNN
                     IANLEKELERATKMAQETKGGILVITEGVFGMSGDLGKLDEIVALKKKFNFRLMIDDA
                     HGFGTMGPTGAGTDEHFGVQKDVDIYFGTFAKSMALIGAFVAAKKEVIDFLRYNMRSQ
                     IFAKSLPMPCVVGALKRLDLIENDPSLRTKLWEVVNAMQSGLKAKGFNLGNSNSPVTP
                     VFLNGSVPEATAIVYDLRENYKIFCSIVTYPVVPKGVIMLRIIPTASHTLKDVERTIS
                     AFTEVAEKLKAGKYQAEKIVNVMN"
     gene            7881..10184
                     /locus_tag="PK252_12615"
     CDS             7881..10184
                     /locus_tag="PK252_12615"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT15595.1"
                     /translation="MKAFQFLACLLFVGLSLNAQHTLWYKQPARQWDEALPIGNGKLG
                     AMIFGRVQYERIQLNEESLWTGSKKDLNKPDAGKYLPEAQKLLFEGKYKEAQQFIEEK
                     MMHPGDWFMYQTLGDLFLKFNHTGEVSNYKYSLSLDSALAHTTYTIDNVNYSREAFSS
                     VDNNCIAIKLCASKEKSISFSAKLSRKKDVTFETAPNGMIIMKGRVTSGDETVIGLNH
                     GVNYEAQLKIVNRGGNIKVQGDSLIVEGADEAIIYIVAATDYWKTDPHAVCSTTLFAA
                     VKKPYEQLKSEMITAHQDLYNRVNLDLGTNERAGWPTDKRIEAFKNDPNDPQLLTTYF
                     NYGRYLLICSSRPNDLPANLQGIWADGLVPPWNADYHININIQMNYWPAEVTNLSECH
                     LPFINFIDSLRPAGRITAKNTYNSRGFVAHFTTDPWYWTGLTGKPEWGMWLMGAAWSC
                     QHIWEHYAFTGDKAFLEKEYPVLKEAALFFVDFLVKDPKTGYLTTAPSTSPENKFIAP
                     DGTITNICTGSTMDMEIIWDLFSNTIEASKILNIDEAFRQQLINKKDSLRPLKIGTDG
                     RLMEWAEPFEEQDPGHRHISHLFGLHPGKEISPALTPELAAAARKTIDFRLSHGGGHT
                     GWSRAWIINFFARLYDGEKAYENLVALLQKSTNINLFDIHPPFQIDGNFGGTAGIAEM
                     LLQSHEGFINLLPALPKAWQNGSVKGLRARGGFEVDMEWKDGKLTSAKIKLDNGNAVY
                     AAYNGKKIMLPAIKGKIFELNTQLEIK"
     gene            10186..11283
                     /locus_tag="PK252_12620"
     CDS             10186..11283
                     /locus_tag="PK252_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15596.1"
                     /translation="MYILYIIGASQAFFLFILSLKNRKNKANAMLSILLLAFALHLLF
                     YCFSIIFNQFASISILRSAFPLLYGPTLYLYIQFQTQDYPKMNVNHYLHFVPFIFFFV
                     SIVVALFINKNFDINNNKSTLYTWGRMIVISSFAFYGVASFQSLKKHKKQVFQKLSYT
                     ENVDLNWVRLLLQSLVFVWGIIIAIVFINKISHFIDIETERAIIYCVICVYVFFIGYN
                     GLRQSAIITVFKDNESLQTKPIRDEQVKDYEQVYFDKIKQLVEEEKLYLNPKLSIKDI
                     AERLKTNVSYVSRAINNKAENNFFGFINQYRVREFERLVIIPEKANYTLLAIALECGF
                     NSKASFNRIVKETTGLTPIELKNKLLHNLRP"
     gene            11486..12547
                     /locus_tag="PK252_12625"
     CDS             11486..12547
                     /locus_tag="PK252_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15597.1"
                     /translation="MKKLALLSLGLYVFVMLSSQDTVKTNNLWGIDKNDRFNEVNCPI
                     GDGYSMWFMRQNLSCNSFQTILETNNVYNSHLESNTPEYRLETYIPVIKSKKFNTIIG
                     SRYWRSSLLQENSEIDNITQFIWIWTTLQFLPTEKWNLMLSSETYQSGNKKSFDEKMS
                     NQFFVVGYAGYSLFDNWQIIGLFRYDMLWEPDGLKRSIIPGLQVKWLPQKNLKLMTGM
                     PVIFATEWTPFEKFDLAAYCWYNMKNSAFIRYRITDKVSVSTIYRASNFKSSDIFGDE
                     KTTTIENKTFSYDNMTQTQHRLALEIGCRFTPNTSIQLTLGRLLYANTKYNLNNNEQR
                     QSTSLKGWYIGASVYFLNF"
BASE COUNT         4208 a         2303 c         2303 g         3691 t
ORIGIN      
        1 atcaaagtta acatcctgtt caacacggcc cattgtaaaa gtaccgcctt caataaacac
       61 acaacccgga ggggtttgtt cttcatactg tgtaaccact tcaaaacctc cattttcttc
      121 actattataa gcccatccgg ttgtttcgga tgtatctccg gtacttgcta aatctgactt
      181 tttgcaggaa aacaaaacca atgttcctgc aattatgagc gaaatgccaa attttgtatt
      241 cataatgctg ttagtattta ttttcaaata taatcatttt tttgttcggc tacaaatcta
      301 tgcaaaaata tcatttttct ggaataataa catcgggaca aggtatcgaa ccaaatcttt
      361 ttcttttagg atcttgctta aaccttgcaa caatggctat ttcgtgtgcc ccggtgtaag
      421 gcataatatt tttcaacgaa aggacattat aatcataact gtacccaatt tgccaattgt
      481 caattataaa accaattgac gctatggcat ttgtcacctg aatcgggaca tttgtcctgc
      541 accacagacc agctatgata ttgttgaact ttaatgatgc accataatca aacatcatac
      601 ttcccccctg atacagtacc attaaagccg gagttagcgt ataaacgggc gctacagttt
      661 tccgatagtt agttaattca atgtcatgcg aataaaatac agagaatttc ataggtgact
      721 caacaaatgt attgttaagc agcaaaaaac tcctattaag gtgtcccaca gatataccaa
      781 actgcgaata tctggtcata gcaagatatc ctaccccaaa atcaggctga gtggagctaa
      841 aatctgcagg caaattgggg tcagcaacat ttaaagatga tgtgtttata ttgtagaact
      901 gaaaagatgc ctgcaacccc agttgcatct tcatgttatg gctttcttta aaacgataac
      961 tatataaaat atcagcctgg gtattggtaa ttacatttcc ggcaatattg tcccttaaaa
     1021 tattcaaccc ccaaccactt ttcatcttag gcgaaaattt atcgtaactg ccttggtaag
     1081 taacataagt agaaccaaaa gccggaagtt ggtttcgata atgtaaaaat acccggccat
     1141 catttactga acctgttacg gcagggtttt caactacaga atttagaaaa tagtgcgaaa
     1201 aacttacatc ttgagcaaca acattaatat aaaacattga aatgcaacct attaatattt
     1261 gctttaaaaa tatctgcctc atccagttaa tacaataatt tgaagtgtat atacgtttgc
     1321 tcaaaaataa tgttttaatc gacataaatt tcttttttaa actacttttg gctctcttta
     1381 ttttatgaca acataaattt atgaaaaaaa gcttatatat cacacttttg ttattgcctc
     1441 aaattgtttt tggtacagac atttcacttt ctaaatattc ggcaacgaca gaaaattctg
     1501 ttttgagtag tggtacatgg tataaaatga agataactaa tgatggcatt tataaactta
     1561 cctattcaca actaaaaaaa tggtttagca aaccttcaaa tgttaaagta tatggtggag
     1621 aaagtaaaat gctttctaaa aacgccaatg aaacaaaaat aaatgattta caacaggttg
     1681 ctttgtatat aaataaaggg agcgacaata cttttaatga aggggattat attttgtttt
     1741 atggtgaagg ccctgataaa tggagctttg atagtacccg aaatatattt aggcatacta
     1801 aacatcaatt ttcagattat aattattatt ttataaccga taacgaaggc gcacaaaata
     1861 tcattcaaga ggccaataat ggttcaaact caagcaataa tatttctatt acaacatttg
     1921 acgactatag ttttattgaa cataatgatg taaacctaat caactcagga gcagaatggt
     1981 atggagacct tattagtaat ggctcaaaga aaactttcag tttcaatttt accaatattg
     2041 tttccttgtc aaaagcttat gtttattacg atttggtgtc aaatgccagt acagaagcaa
     2101 catttacatt gaaatatgga aatgacacaa aaacatttgt tgttacggct ccatttagtt
     2161 ctgaaacagt gggtaataaa aaaactggcg gatattatat agatattacc aattcggcca
     2221 ttaacatttc tgttggttat accaattcgg ccagtagcga aggcagaggt tttatcaatt
     2281 atttaggcat aaatgttatc agagagctga catttgatgg ttcacagcta aagtttcgta
     2341 ctaaggaatc tgccaataac ccggtattga aatattttat aaagaatgga acttcaaata
     2401 ctcttgtttg ggatgttaca gatgccaaaa aaccaaaaac aatatcaacc aatctgaccg
     2461 acaacaatat agttttttcc gattcaggca ataaaataaa gcaatacatt gcatttgata
     2521 acagttctgg ctttttaacc cctgaagtta ttgaaaaacc tatagctaat caaaacatac
     2581 atggtgccaa tactccggat atgattattg ttatccctca agattctgct ttttaccttc
     2641 aagccaaccg attagctgct ttcaggaaac aaaatgataa catcaattgt atggttgttg
     2701 atgtacagca ggtttacaat gaattttcat caggaaaaaa agatgttacg gccatcaggg
     2761 atatggtgaa aatgttttat gacagaggaa atgcagatgg caaccatctt aaatatttat
     2821 tattatttgg aaaagggact tttgccaacc atattgaaaa agaaaataat ttcaacctca
     2881 tcccggtata tcaatcgcaa gaatccctga ataaaggtgg ttcttatgca actgatgatt
     2941 attacggatg gatgaatacg caaagttcag aatccgcaaa tctgcttcaa attgggattg
     3001 gacgactacc tgcaaaaaac gggaaagaag caaaagcact tatagacaaa attatcgctt
     3061 atgacaatcc ttcaaatatt agtgactggc agaatactat aagctttatt gccgatgata
     3121 ttgatgaatc atgggagcat agttttgaag caaattcaga caagtatgca caatatattg
     3181 aaacaatgtt ttcccggttt accattcaaa aaatatactt agatgccttt aaacaaatta
     3241 ccacttccat aggtgaaagc tatcctgagg caaaactaat gtttaataat agaattaaca
     3301 atggagcatt agtagtaaat tatgttggac atggcaatga aaaacaattg gccggagaaa
     3361 acattattga tatttcaagc atcagatcct ggaacaacaa aagtaagctt cctcttttta
     3421 taactgctac atgcgaattt agcaggtttg atgatgtaaa ccttgcagaa tcaaaacaga
     3481 taacctcggc aggagaagaa attatactta accaatatgg aggtggaatt gctttaatga
     3541 ccactacaag acaagtttat aatgatgata acaatatctt aaacgatgcc atcttaaaaa
     3601 atcttttttt aaaagacgaa aatgggaata aactccgaat aggagatgta tttatgaaag
     3661 caaaaaaccg tgcttcaagc actggcctca acaggttaaa ctttactttg cttggcgacc
     3721 cttcattggc ccttaagtat cctgaagaac gcattattgc aattgattcg gtgaatggga
     3781 aaaacaattt tacagatacc attaatgctt tagacttagt gaatatcaaa ggtcaccttg
     3841 aaccccaaga aggcttctcc ggaaatcttt atatcaccat atttgacaaa gaagattcca
     3901 ttgaaaccct tcttaatgac gaagcttcat taccatttca atacgcaaca tggcagaaaa
     3961 taatattcag gggtgaagca tctattgcca atgacagctt ttcctttact tttcccgttc
     4021 caaaagacat aaaataccag gttggtaaag gcagaatggt gttttttgca acggacggaa
     4081 aacaatattt ttcatacgat aataaaaata ttattgtagg aggaattaat aacaatgttg
     4141 caaaaaacta tgaagggcct acaattcagt tattcatgaa tgataccaat tttgtatctg
     4201 gcggcattac cgattctcag cccgctttat tggcccatat caaagatgaa aatggcatca
     4261 attcaacagg taatggcatt ggccatgata taacagctgt gctggatggc gatttattga
     4321 acacttttgt gttgaatgat tattaccgca attctatcga cagctaccag gaaggaactg
     4381 tatattatcc tttctctgaa attgaaaccg ggaaacacca aataaagctg atggtatggg
     4441 atatatttaa caattattcc gaggccaaca tcgactttga agttgtgaac agcgaaagcc
     4501 caaccttgaa aaaaatatac aattatccaa acccaatgag gaactctaca agctttgtcc
     4561 tggaacataa ctggccggat aaaaacttga aagtagaaat catgatatat tcaatgacag
     4621 gtgaaagggt tgcttcgcta aaataccagg gttcatttgc cggatataaa acagctccaa
     4681 taaattggga tggcacaaac caaaacggaa ctaaagtttt tccgggtatt tatatataca
     4741 aagctgtaat aacagataca gacaatcagc aaagttcaaa ttttggaaaa ttggttatag
     4801 tacgttaatt gtaaatttat atatttgtcc actttttaaa aagatcaaga tatgattaga
     4861 aaaacgattt tagtttcagt tatcttattt tcaggaatat gcaatacaat aactgctcaa
     4921 acgcagttag ggcaaagttt ccaagaggat tataatatta tacaaacacc agttccattt
     4981 attaacattg ctcccgatgc aagatctagt ggtatgggcg atttaggagc agctacaaca
     5041 cctgacttaa attcattaca ctggaatgct tcaaaatacg ccttccttga agataaatat
     5101 ggtttgtcaa ccacttacac tccttggctt agagggttat ctaatgatat aaaattatta
     5161 tacctcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gacatttcca
     5281 catactacca tacccctatt agaatgggga gcaacgatgg cgaatgggct atgggtatta
     5341 atatctcaaa tataggttca aaaatttctt ataatgacgg tgatgtaaaa agcttcatac
     5401 ctaccaacct gagattagga acgagattta catacgatat tgaccaatac aatagctttt
     5461 cggctggaat ggatgtgaat aaattgttag tccccactcc tcctatttat agtgattcag
     5521 atagagttac tattgcaaaa gggatgaatc ctgacgtatc tgttgcccag ggaatttttc
     5581 agtcatttta tgatgcccct ggcggaatgt ccgaagaatt gaaagaagtg atgctttcta
     5641 ttggtgcaga atactggtat caaaagcaat ttgccctcag ggccggttat ttcaatgaag
     5701 cagccatgaa aggtaacaga aaatatatga ctgtaggggt tggcttaaag cttaccacta
     5761 ttaccattga ctttgcttac cttataccta atgggggccg cagcaacccg cttgccaaca
     5821 ccatgcgtat cagtgcttca tttgatattg gccatcctaa aaagactaaa aaataatgtt
     5881 caggattggc tttggttatg atgtgcatca acttgttgaa ggccgtggct tttggcttgg
     5941 aggaatagaa ataccccata ccaaaggctc actgggacat tcagacggag atgctctgat
     6001 tcatgctatt tgtgatgcca tactcggagc tgctaacatg cgcgacattg gttatcattt
     6061 tccggacact tcatcagatt ttaaaaatat tgatagtaaa atattgctaa aacgcactat
     6121 tgacttggcc agacaaaaag gctttgaaat tggcaacata gactctacca tttgtcttca
     6181 aaaaccaaag gtcaaagact atattcctgt tatgcagcaa acactggctg aagtgctgtc
     6241 gataagcact aatgatatct ctgtcaaagc tacaactaca gaaaaaatgg gttttgtagg
     6301 acgcgaagaa ggcgttgctg tttacgcagt ggtacttctc gaaaaaaaat agaataataa
     6361 ttactttcga tactaaccct ccacggtctt ttaactgtgg agggttttgt ttattatctg
     6421 taaatcatcc aaaaaaatct tttttttcaa tataacttaa agaaaatctt taattttggc
     6481 gcctgttaaa caaaaaaatg tttcaatcgt ggatatattt gacaaaatag agaaaaaccg
     6541 cggcccactt ggtcaatacc aaaaagaagc cgaaggttat tttatgtttc caaaattagt
     6601 tggagaaatt ggcccataca tgaagttcaa gggcaaagaa gtgttgaact ggagtttaaa
     6661 taactatctt ggattagcca atcaccctga agtacgtaag gctgatgctg aaggcgctgc
     6721 ccagtatggg cttgcagctc caatgggggc ccgtatgatg tccggacaga ctgatttaca
     6781 tgaagaatta gaggcccgtc ttgcaaaatt caccgggcgc gaagctgcat acctggcaaa
     6841 ctttggctac caggctatga tttctatcct tgataccatt ttgaaccgaa atgatgttgt
     6901 tgtttatgac tctgaggccc atgcctgtat cattgatggt ttacgcttgc acttaggaaa
     6961 acgctttgtg tatcctcata acaatattgc caacctcgag aaagagcttg aaagagctac
     7021 caaaatggcc caggaaacca aaggaggtat cttggttatt accgaaggtg tatttggtat
     7081 gtctggcgac ttaggaaaat tggacgaaat cgtagcgttg aaaaagaaat tcaacttccg
     7141 cctgatgatt gacgatgcac atggatttgg aactatggga cctacaggtg ccggtactga
     7201 cgaacatttt ggagttcaga aagacgttga tatttacttt ggtacttttg ccaaatcaat
     7261 ggcccttata ggcgcttttg ttgctgcaaa aaaagaagtt attgatttct taaggtacaa
     7321 catgcgttcg caaatttttg caaaatcact tccaatgcct tgtgttgtag gagctttaaa
     7381 acgtctggat ttgatagaaa atgacccttc tcttcgtaca aaactttggg aagtagttaa
     7441 tgcaatgcaa agtggcttga aagctaaagg tttcaactta ggtaatagca actctcctgt
     7501 aactccagta ttcttaaatg gtagtgtacc tgaagcaact gctattgttt atgatttgag
     7561 ggaaaattat aaaattttct gctctattgt aacctatccg gttgttccaa aaggcgtaat
     7621 catgttgcgt attatcccta cagcttctca cacattgaaa gacgtagaaa gaactatcag
     7681 tgcatttacc gaagtagctg aaaagcttaa agcaggtaaa taccaagctg agaaaattgt
     7741 taatgtaatg aactaagaaa aaacaaagtt ttcaattgat ataaaaactc cggtgaacaa
     7801 ccggagtttt ttgtttataa ataatgagct ttgcgtattt tcattaattt tggtgtttac
     7861 attacactaa acaccaactt atgaaagctt ttcaattttt agcatgtctt ctgtttgtag
     7921 gactatcgct aaatgctcag cacactttat ggtacaaaca gcctgccagg caatgggatg
     7981 aagccctgcc tataggcaat gggaagcttg gcgctatgat atttggacgt gtccagtacg
     8041 agcgaatcca gctcaatgaa gaaagcctgt ggacaggaag taaaaaagac ctgaataaac
     8101 ctgatgccgg gaaatattta cccgaagccc aaaaactact gtttgaaggt aaatacaaag
     8161 aagcacaaca gtttattgaa gaaaaaatga tgcatccggg cgattggttt atgtatcaaa
     8221 cgcttggcga cttgtttctg aaattcaacc ataccggaga agtaagcaat tacaagtata
     8281 gcctgagttt agatagcgct ttagcccata caacctacac aattgacaat gtgaattatt
     8341 cacgcgaagc attttcgagc gttgacaaca actgcattgc cattaaactc tgtgccagta
     8401 aagaaaagag catatcattt tcagctaagt taagccgtaa gaaagatgtt acatttgaaa
     8461 cagccccaaa cgggatgatt atcatgaaag gtcgtgttac cagcggagac gaaacggtaa
     8521 ttggcttaaa ccatggtgtc aactatgaag cacaacttaa gattgttaat cgtggcggaa
     8581 atattaaagt acaaggcgac agccttatag tagaaggcgc agatgaggct attatctaca
     8641 ttgttgctgc aacagattat tggaagacag atccgcacgc agtatgctct acaactttat
     8701 ttgcagctgt taaaaagcct tatgaacaac ttaaaagcga gatgattact gcacatcagg
     8761 atttatacaa ccgggttaat ttagacttag gaacaaatga acgtgctggc tggccaacag
     8821 acaagcgtat tgaggctttc aaaaatgacc ctaacgatcc gcaattgctg actacatact
     8881 tcaactatgg caggtatcta cttatttgca gctcaaggcc aaacgacttg ccagctaacc
     8941 ttcagggaat ctgggctgat ggattagttc caccatggaa tgcagactac catatcaaca
     9001 taaacattca gatgaattac tggccggcag aagtaaccaa tttatcagaa tgtcatttac
     9061 catttattaa ttttattgat agtttacgcc ctgcgggtcg aataacagct aaaaacacct
     9121 ataattcacg tggttttgta gctcatttca ctacagaccc atggtattgg acagggctta
     9181 ccggaaagcc cgaatggggc atgtggctca tgggcgcagc atggagctgc caacatattt
     9241 gggaacatta tgctttcaca ggtgataagg catttttaga aaaagaatat cctgtgttga
     9301 aagaagctgc attgttcttt gttgactttc ttgtaaaaga ccctaaaacc ggatatttaa
     9361 ccactgctcc atctacatcg ccagaaaata aattcattgc tccagatggc actataacca
     9421 acatctgcac aggttcaact atggatatgg aaatcatctg ggatttattt tccaatacca
     9481 tcgaagcgtc taaaatactg aatatagatg aagcattcag gcaacaactc atcaacaaaa
     9541 aagacagctt aaggcctttg aaaattggaa ccgatggaag actaatggaa tgggctgaac
     9601 catttgaaga acaagaccca gggcaccgtc acatatcaca tctctttggc ctgcacccgg
     9661 gaaaagaaat atctccggca cttacaccag aacttgcagc tgctgcacgc aagactattg
     9721 acttccgcct ttcgcatggt ggtggacata cgggttggag ccgagcctgg ataatcaact
     9781 tctttgcccg cctttatgat ggcgaaaaag cttatgaaaa ccttgttgca ttgcttcaaa
     9841 aatcaaccaa tatcaactta ttcgacatcc accctccttt ccagattgac ggaaactttg
     9901 gcggaacagc cggaattgca gaaatgctgc ttcaaagcca cgaaggcttt atcaacctgc
     9961 tgccagcatt gcccaaagca tggcagaatg gctcagttaa aggcttgcgg gccagaggag
    10021 gctttgaagt cgatatggaa tggaaagacg ggaagttaac ctcagccaaa atcaaattag
    10081 acaatggaaa tgcagtttat gcagcttata atggaaagaa aatcatgctt ccggcaataa
    10141 aaggtaagat ttttgagcta aacactcaat tagaaataaa ataacatgta tatcctatat
    10201 attattgggg cttcacaagc tttcttcctt tttatactca gcctgaagaa caggaaaaac
    10261 aaggcaaacg ccatgctaag tattttactg cttgcttttg ccttgcattt gctattttat
    10321 tgtttttcta tcatttttaa ccagtttgct tctataagta tattaaggtc agcatttcca
    10381 ctgctatatg ggcctacatt gtatttatat attcagtttc aaacccaaga ctatcccaaa
    10441 atgaatgtta accattacct ccattttgtt ccattcatct ttttctttgt cagcattgtg
    10501 gttgcactat ttattaataa aaatttcgat ataaataata ataaatcaac actttacact
    10561 tggggacgaa tgattgtaat ttcttcgttt gctttttatg gggttgcatc attccaaagc
    10621 cttaaaaagc ataaaaaaca agtgtttcaa aaattatcct ataccgaaaa tgttgattta
    10681 aattgggtta gacttttgtt gcaatcgctt gtttttgttt ggggaatcat tatagccatt
    10741 gtttttatca ataaaatctc gcatttcatt gatatcgaaa ctgaaagggc tataatttat
    10801 tgcgttatct gtgtatatgt atttttcatc ggctacaacg gattaaggca atctgcgatt
    10861 attacagtat ttaaagataa tgaatctttg caaacgaagc caatccgcga tgaacaggta
    10921 aaagactatg aacaagttta ttttgataag ataaaacagc ttgttgaaga agaaaaactt
    10981 tacctaaacc caaaattatc tataaaagac attgccgaac ggctaaaaac caatgtcagc
    11041 tatgtgtcac gtgcaataaa caataaagcg gaaaataatt ttttcggatt tatcaatcaa
    11101 tacagggtca gggagtttga aagacttgtt atcattcccg aaaaagccaa ttacacccta
    11161 cttgcaatag ctttggaatg cggtttcaac tcaaaagcca gttttaacag gattgtgaaa
    11221 gaaacaaccg gcctcacccc tattgaacta aaaaacaaac tattacataa tttgagacct
    11281 taatttttag gttgtaagac ccatagaacc ctgcaattct ttatcattca acaagcagcc
    11341 aatacttttg tcgtgaaata ttaaaattat tgcacggtga aacacccatg attgctcaat
    11401 agcaaacact aagatattta tttgtgggtg ttctcccgtc aaagacggga caagctatca
    11461 ttcctttaaa ttaaaaataa atatgatgaa aaaattagca cttctttctt taggtttata
    11521 tgtgtttgtg atgcttagct cgcaggatac tgttaaaaca aacaatttgt ggggcattga
    11581 taaaaatgat agatttaacg aagtaaattg ccctattggc gatggctata gcatgtggtt
    11641 tatgcgacaa aacctatcct gcaactcttt tcaaactatt ttggaaacaa acaatgtata
    11701 taatagccat ttggaaagta ataccccaga atatcgcctg gagacctata ttcctgttat
    11761 aaagagcaaa aaattcaaca ccatcattgg cagtcgttat tggcgttcat ctttactaca
    11821 ggaaaatagc gagattgaca acattacaca gttcatttgg atatggacaa ccttacaatt
    11881 cctgcctacc gagaaatgga acctcatgct ttcgtcagaa acttaccaaa gtggcaacaa
    11941 aaaatcgttt gatgaaaaaa tgagcaatca attctttgtg gttggatatg ccggctattc
    12001 gctgtttgac aattggcaaa tcatcggact gttcagatat gacatgcttt gggaaccaga
    12061 tggtttgaaa cgttctataa ttcctggttt acaggttaaa tggcttccac agaaaaacct
    12121 caaactcatg actggtatgc cggtgatttt tgccacagaa tggacaccct ttgaaaagtt
    12181 tgatttggca gcatactgtt ggtacaatat gaagaacagc gcctttatcc gttaccggat
    12241 aactgataaa gtcagtgttt caactattta ccgagcatca aatttcaaaa gcagcgatat
    12301 atttggcgat gaaaaaacca ctaccatcga aaataagact tttagctatg ataacatgac
    12361 ccaaacgcaa catcggttgg cattggaaat tggatgcagg tttaccccaa acacctctat
    12421 tcaattaact ttaggaaggt tattgtatgc taataccaaa tacaacctta ataacaacga
    12481 gcagagacag tcaaccagcc taaaaggttg gtatatagga gccagtgttt actttttgaa
    12541 tttttaaatt gaatcactag tgtcccatta aaatcgggac gttattaaaa attaaacaaa
    12601 tttgg
//
LOCUS       DAOSPF010000081        12604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_4986_length_12604_cov_8.001913, whole genome shotgun sequence.
ACCESSION   DAOSPF010000081 DAOSPF010000000
VERSION     DAOSPF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_12604_cov_8.001913"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1049..2836)
                     /locus_tag="PK252_12630"
     CDS             complement(1049..2836)
                     /locus_tag="PK252_12630"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT15598.1"
                     /translation="MTKNQKLLAWVKEWAELCQPDNVYWCDGSEEENQRLLDQMVKSG
                     AAVKLNESKRPGSYYFQSDPSDVARVEDRTFICSEKKEDAGPTNNWMDPKEMKATLRE
                     MYKGCMKGRTMYVIPFSMGPLGSNIAHIGVELTDSAYVVVNMRIMTRMGKKVLDILGD
                     GEFVPCIHSVGAPLAAGQKDVKWPCAPIEKKYICHFPETNEIWSYGSGYGGNALLGKK
                     CFALRIASNMAKKQGWLAEHMLIMGITNPQGVKKYIAAAFPSACGKTNLAMLIPTIPG
                     WKVETVGDDIAWMKFGADGRLYAINPEAGFFGVAPFTSMDTNPNAMLSAKANTIFTNV
                     GLTPDGDVWWEGIGHDMPAGTIDWKNNVRENASGDPVAHPNARFTSPASQCPAIDKDW
                     ENPAGVPISAILFGGRRPSTVPLVYQSFDWNHGVFLGSIVGSEVTAAAIGLKAGVRRD
                     PFAMLPFCGYHMGDYFGHWVKIGESAPDKTKLPKIFNVNWFRKSAEGKFLWPGYGDNI
                     RVLKWIFERVEGTADAVETPIGYVPTKNSIDISGLAGQAEKMDEVLTVNKEAWKEECE
                     LIAEHQAKFGDRLPAEMKKQFENLKARLA"
     gene            3149..6049
                     /locus_tag="PK252_12635"
     CDS             3149..6049
                     /locus_tag="PK252_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15599.1"
                     /translation="MIFKKNNTIYTNIKSKLAYFTTRLKVTFSKTLMLKDSLDFFSEK
                     AGRVGGELALWVEMMAGSKPQMTYRFEVSKNLMHWFGEVNLFTFLSSKITKPSTYEEF
                     VRFVYERDRAKVIEAHQNAISCQKGYSLEYKICVGFEDCLIIKETVRYFKNRKGEFLI
                     GIITDISAETKARQILQVKERTGALIGEMFKMTGNVKGVEELIYNVMKLISIKLHWNF
                     AHAVMLYNVKFNFSSSHNLYYTKDPLKYDKLVSAMKNQSAIQFSQAQLSVIMNKKSLW
                     LKNIDKNKQYILNELATESGLQHIVIFPLLIRGQVIGLLEFFTSDEDIATELMPDLLD
                     QIGMLLGSVVEQKANEVELKKFSLAIEQNFATVVMTTGEGFVEYVNPMFSMVTGYHPD
                     EVLGKKMSIIKSGVHDYNFYKTLWGVISRGNTWSGEICNKKKNGELFWEQVNISPIKD
                     ENNDISHYVAVKIDITEKRKAEEEILRAKEAAESANKAKSEFLANMSHEIRTPMNAIL
                     GFAELLHAKIEDEQQLSFLDSIKSSGKNLLTIINDVLDLSKVEAGKMVVSKEFVDLFL
                     LLKDIEYMFKLKAVEKGLEFSVEVDFNIPAGIETDEVRLRQILINIIGNAIKFTSNGY
                     VKVKAHSRKISDKFVDITIEVEDTGIGMSKEYLKKLFEPFTQEDSQITKRFGGTGLGL
                     SISKKLIELLNGEIFVESEQGKGSRFTVVFHEVRFTHKEQKELELIQISPENIKFDSA
                     LVLIADDVAANRKYFKSVLQDTNLRIIESENGLETFEKAKIYRPDILITDLKMPGCNG
                     FELFRMLREDHSFDNMKIVATTATASIEERDKLQVHKFDGLLIKPIQVNDVFIELMRL
                     LPHEVIDNTEKIERNNIEEVFNIEQNNEYLFKILESEMMPIWETFESQQPLDEVEGFA
                     KKIREIGQEYSCEPVISYGNLLVRAINNFDIDAMLKNLNDFPKLISTFKKDN"
     gene            6057..7244
                     /locus_tag="PK252_12640"
     CDS             6057..7244
                     /locus_tag="PK252_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOT15600.1"
                     /translation="MQSYTPSDFSILMVDDNPKNLQLLGTNLRNEGYRVEFATNGKMA
                     LSWVEKRVFDLILLDIMMPEMSGYEVCQKIRLKAEYNDVPIIFLTAKVEKESVVHGFK
                     LGAQDYITKPFDIAELMARVRTHLELRYHRKMLIEVNANLEKIVQERTLELQHANKQL
                     SLANDELMKLEKAKAEFLHIIAHEIRTPLNGIKGSVQIMKDLNETASLGNLFKILDES
                     VSRLEKFSIIALKITQLKTGSYELDMQEIPLKILFDKIFAHFAKQINLKKINWKMATC
                     DSCHIWGDNELVSNALISVYDNAIKFTPNEGFIETLVEVRDGKIKLTVKDSGPGFSEQ
                     ALRTLYRLFSPGVKHVNENEGIELAMVKMIMEVHKGDVKIANGEHGANVTLEFPVFDP
                     YKR"
     gene            complement(7246..10095)
                     /locus_tag="PK252_12645"
     CDS             complement(7246..10095)
                     /locus_tag="PK252_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOT15601.1"
                     /translation="MRTFFIAVLLVLLYNFIVAQPVSIGVPFVKNFKPEMYNAADQNW
                     NIVQDQQGMMYFANNNGILCYNGTNWQIAGIASNLSAIHGLAIDKQGRIFAGGYADIG
                     ILKKDKSGNFSWSSLINKIPPQYRKFDYIWNIIPMRRGTVFQSTYMSFYYVDDSIEVI
                     NHPVESIKAFYVEDRLFVLFKDALREISDGKLIDIPLGPSFAPLNVAFMLPFENNTIL
                     VGSESDGLYIYDFKKIVPYKTSIDEFLRKNKLYCGITTRNNQIALGTLHAGLVLIDKS
                     GKMIYNLNTDNGLQNNTVLNIKSDNIGNLWVTMDQGIDYIETASPFTRIPSQKLNGYI
                     YSMVNYKGKLFVATHHGLMYKDWKNIEQINDNNEFSTFPGISEINWSLTVIDDKLFLG
                     HDKGTYIINNNSAQMISNVKGGWCHKLLKHHKDVIVGGTYKALVIYKKKNGNWYQFKV
                     LPDLNESCRIIEEDKNGYLWVTSGYYGVYKVALSENADSITSVEFYDSSKGFPKSLFF
                     GINKINNEIVFGTQAGAFSYNEETNRMEPHKVLFQILKDSHVRKLVQTFDNQVWYVVG
                     DNTGILKYMGDGTIDQVTVPFQKIADNHVPGFENMFFANKNHTFFGTKHGIIIYNSGS
                     TRDYYQKFYTVIEKVFIQGPRDSIICQNLIQKSIDEKVEIPEINYKNNKLHFIFSALF
                     YENPKEITYKYMLEGFDDDWSSWTNKNEKEYTNIPEGTYTFRVKSKNIYGIESEETLM
                     RFVVLAPWYRTKWAIMLYIITIGGAFIFFLKYKNRKFAHEKMLLEQENEKNLRLKTAE
                     HEKEKLEEELKSRQKELASATMNIAQKNEKFLEIKEKLLTIDVQSDKEKRKINSLVKI
                     LDDEINDESYWEQFEMHFNLLNDNFLKKLQKEFPNLTHKDLKMCAFLRMNLSNKEIAS
                     LLNITLRGVEASRLRLRRKLKLEKDSPLTEFIIHF"
     gene            complement(10136..10972)
                     /locus_tag="PK252_12650"
     CDS             complement(10136..10972)
                     /locus_tag="PK252_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT15602.1"
                     /translation="MFTISDIKGSVLLNNGVSMPYLGLGVYKMENNQEVQSSIDFALK
                     HGYRLIDTAALYYNESGVGHSIRNSGIARDQIFVTTKVWNSNQGYDSTLRAFDESIAK
                     LKLDYIDLYLIHWPVKDKYKETWRALEKIYAEKRVHAIGISNFLEHHIKDLFNSAHIK
                     PMVNQIEFHPRLVQQPLLDFCQKEQIVIQSWSPLMQGKYSQITELSAIAEKYQKTVPQ
                     IILRWNLQKGVATIPKSSNPQRIIENANIFDFAISAQDMAFIDNLDKHQRIGADPDNF
                     DF"
     gene            complement(11073..>12604)
                     /locus_tag="PK252_12655"
     CDS             complement(11073..>12604)
                     /locus_tag="PK252_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOT15603.1"
                     /translation="AINKMYKGKSFRFFLCCIFVVPGLLGQQTARIEIINTNSLEFNK
                     QANADAKKLIGDVEFRHESAVMFCDSAYFYTSQNKIDAFGHIAIHQGDTLHLYGDKLM
                     YDGNQRLAQIRGKVKLVDNETVLSTENLDYNIAQDVGYYFNGGNIVNKDNKLKSLEGY
                     YYTKLKMFHFKKNVEIVNPKYVIKSDTLKYNTVKRTAFFFGPSTITSDENSIYCENGW
                     YNTITNKSQFSKNSKLIGTKRLLKGDSLYYERNIGLGKAYGNIFLIDSARNIILTGNY
                     ALYKENPEYAFITDRATLIYIGKTDSLFLHADTLEAFTDTSKINKIVRAYREVRIFKP
                     DLQGICDSMSFSTVDSVLKMFYEPVLWSGANQISADEINMYLANSQISTIDFTANCFI
                     VSKEDTDKFSQVKGKNMKGYFKNNQLVKLSIKGNGQTLYYGKDKEKIVGVNRADCSNI
                     DIILENRKIKKILFLVQPDATLFPIEKLPAEQKLLKGYKWLESLQPKNKEDIYRKTKV
                     K"
BASE COUNT         3745 a         2153 c         2536 g         4170 t
ORIGIN      
        1 gcattatctg gttactttta tgagtgttta cacaatcttt tttatactac caaatcctaa
       61 acacaaaaat cattcatcta aaaataaatg tatattaaac gtttttaact gaacaaagga
      121 ttatacttct tttttttaac atcccattca atttatatta attctaaata aaaaatattg
      181 aatgagttat tgatttgctt ggttttaaat tggtttggtc tgttttatta tgcttggctt
      241 caaacccgaa aattttagaa ggaaagggaa tatgcattac caatggcaaa gacccacacc
      301 tccatccctt ctgttgttta ttttctgtca cactttttgc aagccctccc acaagctgca
      361 catcccgctt tgcttttgtt ttattaaaat tctatggtaa aagcaaatca tgagtaacag
      421 caacttccct caaagcaaaa acccgtataa aatgcattgg agcttaagaa gaattataaa
      481 ctgactagca aaagcaggct actcgcaaaa tcattaaagc tttttaatct ggagatcctt
      541 ctgaagactc tttttgttga aatatgctta taaatgcacc caaaaacgcc aacgtaatat
      601 attgtcaata tatatgcaat tgtattttat gattcataat catgaggccc ccagttcaat
      661 cctgggtcgc gctacttgaa aaataaggca gtacagcatt acgctgactg ccttatttat
      721 tttacatttt cgaatgttat tgtatttcca ttttgtaaca gattagttga cccttactaa
      781 tagccgctaa gcggtgaaat caatattgct tgtaaatatt aagccacgta gataatatgc
      841 tatatcggtg ctacattcct gataaacatg gcaattttgt tgaactacaa agatatcgca
      901 actacatagc tgttttagtt ttgggttgat gtaatttttt aaatcgttaa aatacaattt
      961 ttaatgccta attagggtgt ataaaaataa gagctgccat atcattgata gacagctctt
     1021 gttttattct aactaagttg aaaaaagctt aagctaaacg agctttaaga ttttcaaatt
     1081 gttttttcat ttcagcaggt aaacggtcgc caaatttagc ctggtgttca gctataagct
     1141 cacattcttc tttccatgct tctttgttta cagtaagcac ttcgtccatt ttttctgcct
     1201 ggccggctaa gccgctaata tcaattgaat ttttagtagg gacatatccg attggtgttt
     1261 caacagcatc tgctgtacct tcaacacgtt caaaaatcca tttcaatacg cggatgttat
     1321 cgccgtaacc tggccataag aatttgcctt cagcgctctt gcggaaccag ttaacgttaa
     1381 atattttggg cagcttggtt ttgtcaggag cagattctcc aattttaacc cagtgtccaa
     1441 aataatcacc catgtgatat ccgcagaatg gcaacattgc aaatgggtcg cggcgtacac
     1501 cagctttaag gccgatagca gcagctgtta cttcagagcc tacaattgag ccaaggaata
     1561 caccatggtt ccagtcaaat gactgatata ccagaggtac agtacttggg cggcgtccac
     1621 caaataagat agccgaaatt gggacaccag cggggttttc ccaatctttg tcaatggcag
     1681 ggcattggct tgctggagat gtaaaacggg cattggggtg agcaacaggg tctcctgatg
     1741 cattttcgcg tacgttgttt ttccagtcaa tagtaccggc aggcatatcg tggccaatac
     1801 cttcccacca tacatctccg tctggtgtta aacctacgtt ggtgaagatt gtgttagctt
     1861 tagcgctcaa catagcattg gggttggtat ccattgaagt aaatggagca acaccaaaga
     1921 acccggcttc ggggttgata gcgtacaaac gtccatcagc gccaaatttc atccatgcga
     1981 tatcgtcacc tacagtctca actttccatc ccgggatggt tgggattaac atggccaggt
     2041 ttgttttacc acaagcgctt gggaatgcag cagcgatata tttctttacg ccttgagggt
     2101 tggtaatgcc catgatgagc atgtgttcgg ccaaccaacc ttgttttttg gccatgttag
     2161 aagctatacg caaagcaaag cattttttcc caagaagggc atttccgcca taacctgagc
     2221 cgtatgacca aatttcattt gtttcaggga agtggcagat atatttcttt tcaattgggg
     2281 cacatggcca ttttacatct ttttggcctg ctgcaagggg tgcccctact gagtgtatac
     2341 atggtacaaa ttctccatcg ccaagtatat caagtacttt tttacccata cgggtcatga
     2401 tacgcatgtt tactactaca taagcagagt ctgtaagctc aacccctata tgtgcaatgt
     2461 ttgaaccaag aggccccatg ctgaaaggaa taacatacat tgtacggcct ttcatgcagc
     2521 ctttgtacat ttcgcgaagt gtagccttca tctcttttgg atccatccag ttgttggtag
     2581 ggcctgcatc ttctttcttt tcagagcaaa tgaaagtacg gtcttctacg cgggctacat
     2641 cgcttgggtc actttgaaaa tagtaactac ccgggcgctt gctttcgttt aattttacgg
     2701 cggctccgga tttaaccatt tgatctaaca ggcgttggtt ttcttcttca gaaccatcgc
     2761 accaataaac attgtcgggt tggcacaatt cagcccattc ttttacccaa gcaaggagtt
     2821 tctgattttt tgtcataatt gaattattta tttttattat ttaagtttga atatctgtta
     2881 tttgaataac aaacggcgtt tttgctcaaa ccctgcaaat ataaagtgat attttttttt
     2941 gtgaaagaaa aaacaataat tattgtgaaa aaaaaaacaa taaaaatttt ctggttttta
     3001 attgtactga tttgtgtttg cttgatgcag ttttataact ttaaatattt caaacaattc
     3061 gcaattttta gtaatatgtt catatttggc ctggtaaatt ttgtaactca ataaacatat
     3121 tgtcgtaata aatgttttaa gtatttacat gatttttaaa aaaaacaata ccatatatac
     3181 taatatcaaa agtaagctgg cttattttac gacccggcta aaagttacat tttcgaaaac
     3241 attgatgtta aaggacagtc ttgatttttt ttcggaaaaa gccggccgtg ttgggggaga
     3301 acttgcttta tgggttgaaa tgatggccgg gagtaagcct caaatgactt atcgttttga
     3361 ggtatctaaa aatctgatgc attggtttgg agaagttaat ttatttactt ttctcagcag
     3421 taaaattact aaaccttcga cttatgagga gtttgtgagg tttgtttatg agcgcgacag
     3481 ggcaaaagtg attgaagccc atcaaaatgc tataagttgc caaaaaggct attctcttga
     3541 atacaaaatt tgtgttggtt ttgaagattg tttaattata aaagagactg tcaggtattt
     3601 caaaaaccgg aaaggggagt ttttaatagg tattataact gatatttctg cagaaacaaa
     3661 agccagacaa atattgcagg ttaaagaacg cactggggca ttgatagggg agatgtttaa
     3721 aatgaccggc aatgtgaaag gggtagaaga gctgatttat aacgtaatga aacttatttc
     3781 aataaaattg cattggaatt ttgcccatgc agtaatgttg tataatgtta agttcaattt
     3841 tagcagcagt cacaatttgt attatactaa agaccctttg aaatatgata agcttgtttc
     3901 ggcaatgaaa aaccaatctg ccattcagtt ttcgcaggcg cagctatcgg tcatcatgaa
     3961 taaaaagtcg ctttggctta aaaatattga caaaaacaaa cagtatatac tcaacgaact
     4021 ggccacagag tcagggttac agcatattgt tattttccct ttgcttataa gagggcaggt
     4081 gatagggttg cttgagtttt ttacctcaga tgaagatatt gcaactgaac tgatgcctga
     4141 tcttctcgat cagattggga tgcttctggg tagcgtggta gaacaaaagg ccaatgaagt
     4201 ggaactgaag aaattttcgt tggctattga gcaaaacttt gcaacggttg tgatgaccac
     4261 cggggaaggc tttgttgaat atgtaaaccc gatgttttct atggttaccg ggtatcatcc
     4321 tgatgaagtg cttggtaaaa agatgtcaat cattaaatct ggagtgcatg actataattt
     4381 ttacaaaaca ttatggggag tgattagcag aggcaatacc tggtctggag agatttgtaa
     4441 caaaaaaaag aatggtgagc tcttttggga acaagtgaat atttcgccta ttaaagatga
     4501 aaataatgat atatctcact atgtagctgt aaagatagat attacagaaa agagaaaagc
     4561 tgaagaagaa attttacgtg ctaaggaagc tgctgagtct gccaataagg ctaaaagtga
     4621 gtttttggcc aacatgagcc atgagattcg cacgccgatg aatgccatat tgggttttgc
     4681 cgagttattg catgcaaaaa tagaggatga acagcaactt agctttctcg attcaattaa
     4741 gtctagcgga aagaacctgc tgaccattat caacgatgtg cttgaccttt ctaaagtaga
     4801 agcgggaaaa atggttgtca gcaaagaatt tgtggactta tttttattgc tgaaagatat
     4861 tgaatatatg tttaagctca aagctgttga aaaaggctta gaatttagtg ttgaggtaga
     4921 tttcaatatc cctgccggca ttgaaactga tgaagtaagg cttcgacaaa tcttaattaa
     4981 tataattggc aatgctatta agtttaccag caatggttat gttaaagtta aagcccatag
     5041 ccggaaaata tctgataaat ttgtagatat cactatagaa gtggaagaca caggtatagg
     5101 gatgtcaaaa gaatatttaa agaagctttt tgagcccttc acacaggaag atagccaaat
     5161 tactaaacgt tttggtggta cagggctggg cctgtctatt tctaaaaagc ttatagaatt
     5221 attgaatggt gaaatatttg tagaaagcga acaaggaaaa ggttcgcgtt ttacagttgt
     5281 ttttcatgag gtaagattta cccataaaga gcagaaagaa cttgaactta tacaaattag
     5341 ccctgagaat attaaatttg acagtgccct tgtgcttatt gcagatgatg ttgctgctaa
     5401 ccgcaaatat ttcaagagtg tactgcaaga taccaatttg aggattattg aaagcgaaaa
     5461 cgggctcgaa acctttgaaa aagccaaaat ttatcgtcca gacatactga taaccgatct
     5521 taagatgcca ggttgtaatg gttttgagct cttcagaatg cttcgtgaag atcattcatt
     5581 tgataatatg aagattgttg caacaactgc cacggcaagt atagaagaac gcgataagtt
     5641 gcaggtacat aagtttgacg gtttattgat aaagccaata caagtaaatg atgtttttat
     5701 tgaattgatg aggctattgc ctcatgaggt tattgataat actgaaaaga tagaacgtaa
     5761 taacattgaa gaagttttta atatagaaca gaataatgaa tatctattta aaatccttga
     5821 atctgaaatg atgccaatat gggaaacatt tgaaagccag cagccgcttg atgaagtaga
     5881 gggttttgca aaaaaaataa gggaaatagg gcaggaatat tcttgcgaac cggtgataag
     5941 ttatggaaat cttttagtca gggctattaa caattttgat attgatgcca tgcttaagaa
     6001 tcttaatgat tttccaaaat taattagtac ttttaaaaag gataattgaa aaaagcatgc
     6061 aaagttatac gccatcggac ttctctatat taatggttga tgataatcct aagaatttgc
     6121 agcttttagg gactaacctt agaaacgagg gttatagggt ggaatttgcc accaacggga
     6181 aaatggcctt gtcatgggtc gagaaacggg tttttgactt gattttgctc gatattatga
     6241 tgccagagat gtcgggttat gaggtatgtc aaaaaataag gctgaaagct gagtataatg
     6301 atgtgcctat catatttctt acagcaaaag ttgaaaaaga aagcgttgtg catggtttta
     6361 aacttggggc acaggattat attaccaagc catttgatat agcagagttg atggctcgtg
     6421 tcagaacgca tttagaactt cgttatcacc gaaaaatgct cattgaggtt aatgccaacc
     6481 ttgaaaagat tgtacaagag cgtactttag aacttcaaca tgccaacaaa cagctttcgc
     6541 ttgcaaatga tgagctgatg aagttggaaa aggccaaagc cgaatttttg catatcatag
     6601 cccacgaaat ccgcaccccg cttaatggta tcaaagggtc ggtgcaaata atgaaagact
     6661 taaatgaaac ggcctcttta ggaaatcttt ttaaaatatt ggacgaatcg gttagccggc
     6721 ttgagaaatt ttcaatcatt gccttaaaaa ttacccagtt gaaaaccggt tcgtacgagt
     6781 tggatatgca agaaatcccg ttaaaaatat tgtttgacaa aatttttgcc cattttgcca
     6841 aacaaattaa ccttaaaaaa ataaactgga agatggcaac ctgcgattca tgtcatattt
     6901 ggggtgataa tgaattggtt tccaatgccc tgatttcggt atatgataat gctatcaagt
     6961 ttacccccaa tgaagggttt atagaaactt tagttgaagt gcgcgatgga aaaattaaac
     7021 ttacagtcaa agactcgggg ccagggttta gcgagcaggc cctgaggacg ctttacaggc
     7081 ttttttctcc gggggtaaag catgtcaatg aaaacgaagg tatagagctt gctatggtaa
     7141 agatgattat ggaagtccac aaaggagatg tgaaaatagc caatggagag catggggcta
     7201 atgtaaccct tgagtttccg gtatttgatc cttacaaaag ataatttaaa agtggataat
     7261 aaattcagtc agcggagaat ctttttctaa tttcaacttt cttcttagtc ttaacctgct
     7321 tgcttctacc cctcgcaagg taatattgag caaagaagct atttctttat ttgacaggtt
     7381 cattctgagg aatgcacaca ttttcaggtc tttgtgggtc aggttaggaa attctttttg
     7441 cagttttttc aaaaaattat cgttcaataa gttgaaatgc atttcaaact gttcccagta
     7501 gctttcatca ttaatttcat catcgagaat tttcaccagg gagttgattt ttcttttttc
     7561 tttatccgac tgaacatcga tagtaagtag tttttctttt atttcaagaa acttttcatt
     7621 tttttgggca atgttcatgg tagccgaagc aagctctttt tgccttgatt taagttcttc
     7681 ttccagcttt tctttttcat gttcggcagt ttttagccta aggttttttt cgttttcctg
     7741 ttcaaggagc attttttcgt gggcaaattt gcggtttttg tattttaaga aaaaaatgaa
     7801 ggccccgcct atggtgataa tgtaaagcat aatagcccat tttgtacggt accatggagc
     7861 aagcacaaca aaacgcatta gagtttcttc gctttcaatt ccataaatat ttttagattt
     7921 taccctgaag gtgtaagtac cttcgggaat gttggtgtat tctttttcat ttttgttggt
     7981 ccaggagctc cagtcatcat cgaaaccttc aagcatatat ttgtaggtta tttcttttgg
     8041 gttttcataa aaaagggctg aaaaaatgaa atgcagcttg ttatttttat aatttatttc
     8101 gggtatttca actttttcgt caatagactt ttgaatcagg ttttgacaga tgatagaatc
     8161 gcgagggcct tgaataaaaa ctttttcaat aacggtgtaa aatttttggt agtaatcgcg
     8221 ggtacttcct gaattgtaga ttattatccc gtgttttgtc ccaaaaaagg tatggttttt
     8281 attggcaaaa aacatgtttt caaaacccgg gacatggttg tcggcaattt tttgaaaagg
     8341 gactgttacc tggtcaatag ttccatcacc catatatttt aatatcccgg tattgtcccc
     8401 aacaacgtac caaacctggt tgtcaaaggt ttgaacaagt tttcgtacgt gcgaatcttt
     8461 aagaatttga aataacactt tgtgaggttc catgcggttg gtttcttcat tgtaagagaa
     8521 tgcgcccgct tgtgtgccaa aaacaatttc gttgtttatt ttatttattc caaaaaataa
     8581 gctttttgga aagccttttg aagaatcgta gaattcaaca gaagttatgc tatcagcgtt
     8641 ttccgaaagg gctactttat atacgccata gtatccgctg gtaacccaca aatagccgtt
     8701 tttgtcttct tcaataatgc ggcacgattc gttcaaatcg ggtaaaacct tgaattgata
     8761 ccagttcccg tttttctttt tgtaaataac aagggcttta tatgtcccgc caacaattac
     8821 atctttgtgg tgttttagaa gtttgtggca ccatccgcct ttaacgttag aaatcatttg
     8881 ggccgagttg ttgttgatga tgtaggtccc tttgtcatgc cccaaaaata gtttgtcgtc
     8941 aatgactgtt agcgaccagt ttatttcgct tattcccggg aaggtagaaa actcgttatt
     9001 gtcattgatt tgttcaatgt ttttccagtc tttgtacatg aggccatgat gtgttgcaac
     9061 aaagagtttg cctttgtaat tgaccatgga ataaatgtat ccattaagtt tttgtgaggg
     9121 tatccgtgta aaaggtgaag ctgtttcgat ataatctatt ccttgatcca tggtaaccca
     9181 gaggttcccg atattgtcag atttgatgtt caggacagta ttgttttgta agccattatc
     9241 ggtgttgagg ttgtaaatca tcttgccaga cttgtctatc agtacaagcc cggcgtgcaa
     9301 tgtacctaag gcaatttggt tatttcgcgt tgtgattccg cagtagagtt tgttttttct
     9361 caaaaattca tcaatgctgg ttttataggg tacaattttt ttaaagtcat aaatatatag
     9421 tccgtcagac tctgagccga caagtatagt attgttttca aaaggcagca tgaaagctac
     9481 attaagaggg gcaaaggaag gccccagggg aatgtcaatc agctttccat cggatatttc
     9541 gcgtaaggca tctttgaaaa gtacaaaaag cctgtcttct acgtaaaaag cttttatcga
     9601 ttcgacggga tgattgatga cttcaatgct gtcgtcaaca tagtaaaagc tcatgtaggt
     9661 ggattgaaac actgtacccc ttcgcatggg gatgatgttc cagatatagt caaatttgcg
     9721 gtattgagga gggattttat ttatcaggct tgaccatgag aagtttcctg atttatcttt
     9781 ttttagtatg ccaatatcgg cataccctcc ggcaaatatc cggccttgct tgtcaattgc
     9841 cagcccgtga atggctgata agtttgaggc aatccctgct atttgccagt ttgtgccatt
     9901 gtagcaaagt attccgttat tattggcaaa atacatcatc ccttgttggt cttgtacaat
     9961 gttccagttt tggtcggcag cattgtacat ttcgggtttg aagtttttga caaaagggac
    10021 cccaatgctt acgggttgtg caacaataaa attatagaga agaactaata aaacagcgat
    10081 aaaaaatgtt ctcataatat ttatcttaaa gaaaagacaa atataatgtt tgcatttaaa
    10141 aatcaaagtt atcggggtca gccccaattc tttgatgttt gtcaaggttg tcgataaatg
    10201 ccatatcctg ggctgaaata gcgaagtcga aaatgtttgc attttctatt atcctttgcg
    10261 gatttgacga tttggggata gtagctacgc ctttttgcag gttccagcgg aggataattt
    10321 gagggactgt cttttgatat ttttcagcta tggcagacag ttctgttatt tgtgagtact
    10381 tgccttgcat gagagggctc catgattgta ttacaatttg ttctttttgg cagaaatcaa
    10441 gcagtggttg ttgtaccaag cgggggtgaa actctatttg gtttaccatg ggtttgatgt
    10501 gggcagagtt gaaaaggtct ttaatatggt gctcaaggaa attgctgatg cctattgcgt
    10561 gtacacgttt ttcggcatat attttttcaa gggcgcgcca tgtttcttta tacttgtctt
    10621 ttaccggcca gtgtatgagg tataaatcaa tgtagtcaag ctttaatttg gcaatgcttt
    10681 catcaaaggc ccggagggta gaatcgtatc cctggttgct attccaaacc tttgtggtga
    10741 caaatatttg atcacgtgct atgcctgagt ttcttatgct atggccaact ccactttcat
    10801 tataatataa agccgccgtg tctatcaacc ggtagccatg ctttaatgca aaatcaatac
    10861 ttgattgaac ttcctggttg ttttccattt tgtacacgcc caatccgaga taaggcattg
    10921 aaactccatt gtttaaaagg acagaacctt taatatcaga tattgtaaac atagtatcgt
    10981 ttttttataa aaataatcac tattgtttga aaacaccaat agtttaaaaa tgttgatttc
    11041 aataataacc ttttaaatgg tttttataaa agttacttga ctttagtttt tctgtaaatg
    11101 tcttctttgt ttttgggttg cagagattca agccatttat agccttttag caatttttgt
    11161 tcggctggta atttttcaat tgggaataaa gtggcatcgg gttgaacgag aaataggatt
    11221 ttttttattt ttctattttc gaggataatg tcaatatttg agcagtctgc acggtttacg
    11281 cctactattt tttctttgtc ttttccgtaa tatagcgttt gtccgtttcc tttaatgctg
    11341 agtttcacta gctggttatt tttgaaatat cctttcatgt tttttccttt tacctggcta
    11401 aatttatcgg tatcttcttt agaaacaata aagcaattgg ctgtgaagtc gatggtgctt
    11461 atctggctat tggcaaggta catgtttatt tcatcagcgc taatttggtt agcgccagac
    11521 cataaaacgg gttcgtaaaa cattttcagt acagagtcaa cagtggaaaa agacatagag
    11581 tcgcaaatgc cttgtaagtc gggcttaaat atgcgaactt cacgataggc tcttactatt
    11641 ttattaattt tagaggtgtc agtaaaggct tctaaggtgt ctgcatgcaa aaatagcgag
    11701 tctgttttac cgatgtatat taaagttgca cgatcggtga taaatgcata ttcgggattt
    11761 tctttgtata aagcgtaatt accagtaagt attatgttcc gggccgaatc tataaggaaa
    11821 atgtttccat aagcttttcc taatcctata tttctctcgt aataaaggct atcgcctttt
    11881 aaaagtcgtt ttgtacctat aagtttagaa tttttactaa actgcgattt attggtgatg
    11941 gtattatacc agccattttc gcaataaatg ctgttttcgt cagaagtgat agtacttggc
    12001 ccaaagaaaa aagcagtgcg tttgacagtg ttgtatttga gcgtatctga ttttataaca
    12061 tatttagggt taacgatttc aacgtttttt ttaaaatgaa acatttttag tttagtatag
    12121 tagtatcctt caagactttt gagtttattg tctttattta caatatttcc tccattgaaa
    12181 taatatccaa catcttgagc aatattatag tcaaggttct cggtagagag tacagtttcg
    12241 ttatcaacca atttgacttt tcctcttatt tgggcaagac gctgattgcc atcgtacatt
    12301 agcttgtcgc catagaggtg cagcgtgtcg ccctggtgaa tggcaatgtg cccaaatgcg
    12361 tctattttgt tttgtgaggt ataaaaatag gctgagtcac aaaacataac agcgctttca
    12421 tgcctgaatt ctacatcacc aatgagcttc ttagcatctg cattggcctg tttattaaat
    12481 tctaaactat ttgtattgat gatttctata cgtgctgttt gttggcccaa taaacccggg
    12541 acaacaaaaa tgcagcacaa aaagaaccta aaactcttgc ctttatacat tttattaatg
    12601 gcta
//
LOCUS       DAOSPF010000082        12302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5139_length_12302_cov_8.018127, whole genome shotgun sequence.
ACCESSION   DAOSPF010000082 DAOSPF010000000
VERSION     DAOSPF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_12302_cov_8.018127"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1560
                     /locus_tag="PK252_12660"
     CDS             <1..>1560
                     /locus_tag="PK252_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15604.1"
                     /translation="KDSVCYLMHKIDIDNKGIKYLTVVSDFTLAKNKYMLSYNFMIES
                     FFSKFIFNAFNILLFNFIILLVVVILYLKLLRWRDVQEKTLRESEEAFKQIIELIPIG
                     IIIIDKASKIKTINKAALKILMIEDASDILGTDISHKFLVRKALVVGEDYASAYETDH
                     FLKYEKEGKEVIIYKRDIPLFLKGEEVIVQSYIDVTPIEKSRQREMAANMAKSEFLAK
                     MSHEIRTPMNGIIGMADSLMREPLGVEQTEQVNIIKKSADLLLNIINDILDLSKIEAG
                     KMVLEEIPFKLHDEVKLIAELFAPAAMEKDVLLKYTIDSDIPDKLIGDPFRLRQIIIN
                     LIGNSIKFTQEGEIWIKVSKMEEYNRNLVLKFVIEDTGIGIPKEKINHIFQSFTQADN
                     STTRKYGGTGLGTSIAKQLVELMHGEISVDSPSPISTNPRYPGAVFTFTVELYSDEPN
                     TKSIDNSDIKSFTQIKTLIIGENKTDEKQICDTFSLFNVPTESVVFQKSTIDILKKNF
                     KESPDKYKLII"
     assembly_gap    1561..1660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1673..2158
                     /locus_tag="PK252_12665"
     CDS             1673..2158
                     /locus_tag="PK252_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT15605.1"
                     /translation="MTTGEYNESVNLYADNIYRFILKNIKDQDKAKDIVQDTYEKLWL
                     RVETVSYEKARSYLFTTAYHTMIDMLRRDAKQSSMESVSPSEYSHSEQYSDLHEVLNQ
                     AIQLLPEAQRSVIMLRDYEGYSYEEIAEITGLSISQVKVYIFRGRIFLKEYIGKVETV
                     L"
     gene            2155..3225
                     /locus_tag="PK252_12670"
     CDS             2155..3225
                     /locus_tag="PK252_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15606.1"
                     /translation="MMRAMKINIDNYEAWLLDYLEGNLGPEDTALLLEFMALNPDLRP
                     DFDEIKQIVPEPAPAFSEKIFLKKTGIAEIDDMDAFERTCILAVESQLDKKDKQQFDS
                     YLLQHPQKRKTYEQYAKTHLMPDESIVFNNKSKLKHLQISKGRVIVWSSVAAAAASVI
                     ILLFSMPADNPDKVVQKVINLPKNEVVAVQKTAEAPVLESKQIAKMLKNPKKEQFAQK
                     TDTVEAVNKNLREEEIVLEPILAIEAKPLENIEAEIVAHLPVQYLKTNMPVGVNEQYA
                     FRDITLPELVSNRFNEFIKDKSIANDNVVNFWSVANFALKKINQAAGTDLKLKKAYDE
                     KNGKVIYVMKTDLFSFYREKQK"
     gene            3334..4380
                     /locus_tag="PK252_12675"
     CDS             3334..4380
                     /locus_tag="PK252_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT15607.1"
                     /translation="MKKIYLLGIVFLVAVVGKSQDVGNTALINRDDNAVVSEDSSKAA
                     SGKTANEYYAEKDSDRQILGDRPKVVKTNGDTTRIALGKKGITIVEKNGKTTVQIDNI
                     ENDKDNNDNSNNDSYPEVPEAGFPRWQEHKFHSPKFEPHWAGIEIFLNNFVTSGLSLN
                     LPKESQFLELDAAKSIGVRVNILEFPIAITNEQGFSTGLGFEFNSYYFSSDTNNIQKS
                     NGYIVYKNKPAGAGNYSKNKIKDTYLNVPLFYEIQFPLGSSKRPLYFSFGVIGGLKLG
                     STTKEYYTLNGEDKKDITKGDFYMSPIRLAYQARIGYRRLHLFATYYDTPFFRKGKGP
                     EVHPFDIGIMLLNW"
     gene            4749..5303
                     /locus_tag="PK252_12680"
     CDS             4749..5303
                     /locus_tag="PK252_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT15608.1"
                     /translation="MDDFSFLLEHIKNNNEQAFVRLVEAYNGMVFQLAFSFLKNTEEA
                     EDLTQDVFLSVYENIGQFRNESSLKTWIYRITVNKSLNRQRKLKWCRFFRPIGSVPSD
                     DKNLITEDTNPQYAVLNKELKAKLTNAIDNLPERQRTAFTLYKYQDLGHQEIAEIMGL
                     SVSAVESLIHRAKQNLQAALAEFR"
     gene            5357..5755
                     /locus_tag="PK252_12685"
     CDS             5357..5755
                     /locus_tag="PK252_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT15609.1"
                     /translation="MKCNKKYDFFAYQKRNISPVLLREIENHIAGCDKCRNVSMQVKL
                     LEMAINEQKLIRPDAFMSQRIVAKITNRQSFAGILRPMPAFAIIILLALFVGIGIGIF
                     ATKSSQQTIEVFSWNDQEQEVIEMAFLNEN"
     gene            5761..6312
                     /locus_tag="PK252_12690"
     CDS             5761..6312
                     /locus_tag="PK252_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15610.1"
                     /translation="MGNNLSTNKKLIWTIIALALLNIATISTVFWRFYHDGHDRFEKQ
                     RHERRVENPDRMKYFIKDKLHLSDHQFAQFEKFDAVFKQQTKQCFDKMEDIRHSMLNE
                     MTSEKPDTARLMAYSNELGQQHIILKKHLIEMYFNLKSICDKEQQQKLSAIFKNMIAR
                     DNMQPSMRGMKRKTDGCRSSKHE"
     gene            6721..6791
                     /locus_tag="PK252_12695"
     tRNA            6721..6791
                     /locus_tag="PK252_12695"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6753..6755,aa:Gln,seq:ttg)
     gene            complement(6969..8774)
                     /gene="typA"
                     /locus_tag="PK252_12700"
     CDS             complement(6969..8774)
                     /gene="typA"
                     /locus_tag="PK252_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT15611.1"
                     /translation="MTNIRNIAIIAHVDHGKTTLVDKMILAGKIFKDHENPGDLIMDS
                     NELERERGITILAKNVSVIYKDTKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALSMGLKPIVVVNKVDKPNCRPDEVQEQVFDLMFHLGANEDQLNFP
                     TLFGSGKNGWMSNDWRKPTTDIVALLEAIIKYIPAPKVTEGTPQMLITSLDYSSYVGR
                     IAIGRLQRGTLKEGMAISLVKRDKTIVKSRIKELYLFEGLQRKKVEIVNAGDICAIVG
                     IEGFEIGDTVADYDNPEGLKPISIDEPTMSMLFTINNSPFFGKDGKFVTSRHIKERLD
                     RELEKNLALRVEQTDSADSFVVFGRGVLHLSILIETMRREGYELQVGQPQVIIKEING
                     EKCEPVEFLTIDVPDEFSGTVIDMVTKRKGNMVNMERRDERVIIDFIIPSRGIIGLRS
                     NLLTATTGEAIMSHRFKDFEPLKGNIERRINGSLIAMETGTAIAYALDKLQDRGIFFV
                     YPGEEIYEGQVVGENTRPDDLCLNLTKTKKLTNMRATGSDEKMKIAPPVRFSLEEALE
                     YIQEDEYVEVTPKSIRLRKIILNENERKRVGKKVS"
     gene            8928..9383
                     /locus_tag="PK252_12705"
     CDS             8928..9383
                     /locus_tag="PK252_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOT15612.1"
                     /translation="MERSKRLIVNFKGLADGKHQFTFERKDDFFAAIEYSEFEKGEID
                     VKVILDKGFSNITLDVSIKGQVMVMCDRCLDPYYEKIKFDGTLYAKYDDAGTDADEEI
                     ILLGKNDFEIDITHYVYESVCLSLPYQRVHKKGACNEQMIELIKKHKSN"
     gene            9410..9595
                     /gene="rpmF"
                     /locus_tag="PK252_12710"
     CDS             9410..9595
                     /gene="rpmF"
                     /locus_tag="PK252_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT15613.1"
                     /translation="MPNPKHRHSRTRRDKRRTHYKAVAPTTATCSNCGAAVLYHRVCP
                     ECGYYRGKMAVEKEVAA"
     gene            9612..10556
                     /locus_tag="PK252_12715"
     CDS             9612..10556
                     /locus_tag="PK252_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOT15614.1"
                     /translation="MIKVGIDISGGDFAPEATVKGAILAQKELPSNVKLVCIGDKAQI
                     DAVCAREGFDNSVWEIVHTTENIGMGEHPAKAYQQKENATIPLGFKLLKTGKIDGFAS
                     AGSTGAMMVGAMMVAKVVPNVIRPSIATFMPTDSDIPTLLTDVGINPDTKPDVLLQYG
                     ILGSLYVQHVFNIKNPRVALLNIGSEAEKGNLLTKAAYPLFKECKSINFVGNIEGHDF
                     YSNEKADVVVCDGFVGNVVLKGSEAFYELLKKRNIHDPFFEKFNYENFGGTPILGIDA
                     VAVIGHGISNDIAIKNMILQTMHVAQAGLVEKIKEAFN"
     gene            10556..11551
                     /locus_tag="PK252_12720"
     CDS             10556..11551
                     /locus_tag="PK252_12720"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOT15615.1"
                     /translation="MTKFRAAITGIGAALPDYVLTNDELATMVETSDEWIMQRIGIRE
                     RRILKGEGKGASDLGAEAVNQLFQKTGLKPEEVELVVCATITGDHPFPATANIICDKV
                     GIKNAWSFDISAACSGFIYALEIAAKFVESGEYKKVVVVTPEKMSAITNYKDRTTCPL
                     FGDAAAAILLEPATEEVGVMDYILHADGVGRQYLYMKSGGSVSPASHKSVDNDEHFVY
                     QEGQTVFKYAVTRMADVAAEIMEKNKLNPDTTWLVPHQANIRIIDAVARRMNVSSEKV
                     MINIQKYGNTTAATIPLCLWEWEKQLKKGDDLVLVAFGGGFTWGSIYLKWAYDSK"
BASE COUNT         3896 a         2184 c         2479 g         3643 t
ORIGIN      
        1 caaagactct gtatgttacc ttatgcataa aattgatatt gacaataaag gtattaaata
       61 tttgaccgtg gtttccgatt ttaccctggc caaaaacaag tatatgcttt catataattt
      121 tatgattgaa agcttttttt ccaaatttat ttttaatgct tttaacatcc tcttgttcaa
      181 ttttatcatc ctgttagttg tagtcatact ttatttaaaa ttgcttaggt ggcgtgatgt
      241 ccaggaaaaa acattgcgtg aatctgaaga agcctttaaa caaatcatcg agctcattcc
      301 tattggtatt atcatcattg ataaggccag caaaataaaa acgataaata aagcggcctt
      361 gaaaatcctg atgattgaag atgctagtga tattctgggt actgatatca gccacaaatt
      421 tttggtccga aaagctttag tagttggtga agattatgct tcagcttacg aaacggacca
      481 ttttttgaaa tacgaaaaag aaggcaagga agtcattatt tataaacgtg atattccttt
      541 attcctcaag ggcgaagaag taattgtaca atcttatatt gatgttacgc caatagaaaa
      601 gtcacgccaa cgtgaaatgg ctgccaatat ggctaaatct gagtttttgg ccaagatgag
      661 ccacgaaatt cgcaccccaa tgaacggtat aattggcatg gccgactcgc tcatgcgtga
      721 accgcttggt gttgaacaga ctgagcaggt aaacatcatc aaaaaatccg ctgatttgtt
      781 gttgaacatc atcaatgata ttttggactt gtctaaaatt gaagcgggca aaatggtgtt
      841 ggaagaaata ccttttaaat tgcatgatga ggttaagctt attgccgaat tgtttgcccc
      901 tgctgctatg gagaaggatg tattgcttaa atacactatt gatagcgata ttccggataa
      961 acttatcggc gaccctttcc gcttaaggca aattattatt aatcttattg gcaattctat
     1021 caaatttacc caggaagggg aaatatggat taaggtttct aaaatggaag aatacaaccg
     1081 taaccttgtg ttgaagtttg tgattgaaga tactggcata ggtattccta aggaaaagat
     1141 aaatcatatt tttcagtcgt ttacccaggc agacaacagc acgacccgca aatatggtgg
     1201 aactggcctt ggtacatcta ttgcaaagca attggtagag cttatgcatg gtgaaatatc
     1261 tgtagatagc ccttctccta tttctacaaa tccgaggtac ccgggtgctg tcttcacttt
     1321 tacggtggag ctttattcgg atgagccaaa tacaaaatct attgataatt ctgatattaa
     1381 atcttttacc caaatcaaaa ctttaatcat tggcgaaaac aaaaccgatg aaaaacagat
     1441 atgtgatact ttttcacttt ttaatgtgcc aacagaatct gttgtttttc agaaaagtac
     1501 aatagatatt ttaaagaaaa actttaaaga aagcccagat aaatataagt tgattatcat
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taaagaaaaa cgatgactac
     1681 tggtgagtac aacgaaagtg taaaccttta tgccgacaat atctatcggt ttatcctgaa
     1741 aaatatcaaa gaccaggata aagccaaaga tattgtgcag gatacatatg aaaaattgtg
     1801 gctcagggtc gaaacggttt cttacgagaa agcccgttcg tatcttttta ccacggcgta
     1861 tcataccatg attgacatgc tgcggcgtga tgccaaacaa agctcaatgg agtctgttag
     1921 cccttcggag tattcgcatt ccgagcaata ctcggatttg cacgaggtgc ttaatcaggc
     1981 catacagtta ttgcctgagg ctcaacgttc ggtcatcatg ctccgcgatt atgaagggta
     2041 ttcgtacgaa gaaatagccg agataaccgg gctcagcatt tcgcaggtga aagtttacat
     2101 ttttcgcggg cgtatttttc tgaaagaata cataggtaaa gtagaaacag tattatgatg
     2161 agggctatga aaatcaatat agacaattac gaggcatggc tgcttgatta cttagaaggg
     2221 aacctgggcc ctgaagatac tgctttgctg cttgagttta tggcgctaaa tcctgatttg
     2281 aggcctgatt ttgatgagat aaaacaaatt gtcccagagc cagcccctgc cttttctgaa
     2341 aaaatatttt tgaaaaagac gggtattgcc gaaatagatg atatggatgc gtttgaaagg
     2401 acatgcatcc tggctgtcga aagtcagttg gacaaaaagg acaagcaaca gtttgacagt
     2461 tacctgttgc aacatcccca aaagcgtaaa acttatgagc agtacgccaa aacacatttg
     2521 atgcccgatg aaagtatcgt atttaataat aagtctaagc ttaaacatct gcaaatatct
     2581 aaaggacggg ttattgtttg gtcttctgtg gctgcagccg cagcttctgt aattatttta
     2641 ttattcagta tgcctgccga taatccggat aaagttgtcc aaaaggttat aaaccttcca
     2701 aaaaatgaag ttgttgcggt tcaaaaaact gctgaagctc ctgttttaga aagcaaacag
     2761 atagctaaaa tgctaaaaaa cccgaaaaaa gagcaatttg cacagaaaac cgatactgta
     2821 gaggctgtaa ataaaaacct gcgtgaagaa gaaattgtct tagagcctat attagctatt
     2881 gaagctaagc ctcttgaaaa tatagaagct gaaatagttg ctcatttacc tgtacaatat
     2941 cttaaaacca atatgcctgt tggtgtaaat gaacagtatg ctttcagaga catcacttta
     3001 cctgaattgg taagcaacag gtttaatgaa tttattaaag acaaaagcat tgccaatgac
     3061 aatgttgtaa acttctggtc agtagccaac tttgctttga aaaaaattaa ccaggcagca
     3121 ggtactgatt tgaagctcaa aaaagcttat gatgaaaaaa acggcaaggt gatatacgtg
     3181 atgaaaacag atttattcag tttttatcgc gaaaaacaaa aataagtgta acaaaccgca
     3241 gagctcgtaa gtcatacttc aaaaccgatt aaaaatatgc tttttgaaag aatgtataaa
     3301 agttgattgt tgaacagaaa aaattattta cagatgaaaa aaatatattt attaggaatt
     3361 gtttttttgg tggcagttgt tggcaaaagt caggatgttg gcaacacagc tttaattaac
     3421 agagatgata atgccgtggt atctgaagat agctcaaaag ccgcgtcagg taaaactgca
     3481 aacgaatatt atgctgagaa agattcggac cggcaaatat taggagatag gcctaaagta
     3541 gtaaaaacaa acggggacac tacccgcatt gcccttggta aaaaaggcat cactatcgtt
     3601 gaaaaaaatg gcaaaactac tgttcagatt gataatattg agaatgataa agacaataat
     3661 gataacagca acaatgatag ttaccctgag gtaccggaag ctggcttccc gcgatggcag
     3721 gagcataaat ttcatagccc gaaatttgaa cctcattggg ccggcatcga aattttcctc
     3781 aataattttg tcacttccgg tttgtctttg aacctgccca aagagagcca atttttggag
     3841 ctggatgctg ccaaatctat cggggtaagg gtaaacattc ttgaatttcc tattgccatt
     3901 accaatgaac aaggtttttc tactggtttg gggtttgaat ttaatagtta ctatttctcc
     3961 agcgacacaa acaatatcca aaaaagtaac ggctatatcg tttataaaaa caaacctgcc
     4021 ggggcaggaa attattcaaa aaataaaatt aaagatactt atctcaacgt accattgttt
     4081 tacgaaattc agtttccgtt aggaagtagc aaaaggcctt tgtatttttc ttttggggtt
     4141 attggcggat taaagctggg ttcaaccacc aaagaatact ataccctcaa tggagaagac
     4201 aaaaaagaca taaccaaagg cgatttttac atgtctccta tccgcctggc gtaccaggca
     4261 agaataggat accgccgtct tcaccttttt gctacttact atgatactcc atttttcaga
     4321 aaaggcaaag gccctgaagt gcatcctttt gatataggca ttatgctcct caattggtag
     4381 taccctggta gtactttata tgcagtgcag cctgtgtaaa agtgggctgc acttttttta
     4441 caataggatc gtctttaaac acttttttta ttcaactcaa attcaatatt aaaaaatact
     4501 taatctatta agaaattgta atcctgcaat ttaataaatt gcatcaaaca attcttaaat
     4561 cagcttttcc tttttataat gggagcgtta accccactaa atcatataat tcgacaaaat
     4621 attgccttct aataattaat ttgggttgaa aaaaattata tcaaaagaaa aacgtgtttt
     4681 gtgtgtcaaa ataacatatg ctgttgaata aaatagagaa tatcccgtta tatcggtatt
     4741 aaagttatat ggacgatttt tcatttctgt tagagcatat caagaataat aacgaacagg
     4801 cttttgtcag gttagtagaa gcctataatg gtatggtttt tcaattagct ttttcgtttt
     4861 tgaaaaatac cgaagaagca gaagacctca cccaggatgt ttttctttcg gtatatgaaa
     4921 atataggcca atttcgcaac gaatcatctt taaagacatg gatataccgt ataaccgtca
     4981 ataagtcgct aaacaggcag cgaaagctca aatggtgccg cttttttcgc cctataggat
     5041 cagttccgtc agatgataaa aacctgatta ctgaagatac taatccgcaa tatgccgttt
     5101 taaacaaaga attgaaggct aagcttacta atgccattga caatttgcct gaaaggcagc
     5161 gtacggcttt taccctttac aaatatcaag acctggggca ccaggaaata gccgaaatta
     5221 tgggtttatc tgtttcggct gtagagtcgc ttatccatag ggcaaaacaa aatttgcagg
     5281 cagctttggc agaattccga taattttatg caagaaaaac gaaatgtaat tgtctattat
     5341 atagaatatt taaaaaatga aatgtaacaa aaaatatgat ttttttgctt atcaaaagag
     5401 aaatatatct cctgttttgc tacgcgaaat tgaaaaccat attgccggtt gtgacaaatg
     5461 cagaaatgta tctatgcagg taaagctttt agaaatggct ataaatgagc aaaagctcat
     5521 aaggcctgac gcttttatgt cgcaacgcat tgtggctaaa ataacaaaca gacaatcgtt
     5581 tgcaggtatt ctgaggccaa tgcctgcatt tgcaataatt attttattgg cattatttgt
     5641 aggaatcgga attggcattt ttgccaccaa atcatcacaa caaactattg aagtttttag
     5701 ttggaacgat caggagcagg aagtaataga aatggcattt ttaaacgaaa actgataaaa
     5761 atgggaaaca acctaagtac aaataaaaaa cttatatgga caattattgc tcttgcctta
     5821 ttgaacatag caacaataag tactgttttt tggcgttttt accatgatgg acatgacagg
     5881 tttgaaaagc agcgccatga aaggcgcgtt gaaaatcccg acaggatgaa atattttata
     5941 aaagacaagc ttcatctttc agaccatcaa tttgcccaat ttgaaaaatt tgacgctgtt
     6001 tttaaacaac agacaaaaca atgttttgat aaaatggaag acatcaggca ttctatgctt
     6061 aacgaaatga catcagaaaa acccgataca gcacggctta tggcctatag caatgaattg
     6121 gggcaacaac atatcatcct gaaaaaacat cttattgaaa tgtactttaa tcttaaaagc
     6181 atttgtgata aagagcaaca gcaaaaatta tctgcaattt ttaaaaatat gatcgcacgc
     6241 gataatatgc aaccttctat gcgtggcatg aaaagaaaaa cagatggttg ccgttcctct
     6301 aagcacgagt aagccttttg tttgtttaat tgtttaaggt taatgtttgg ttttccggtc
     6361 tgtacttagt aagtataggc cggatttttt ataaacccaa ctaaatcata tagttcggct
     6421 ttataaagcc ttttaatgat taatttggga ttataagggt gaaagaatta aggcgttatg
     6481 agttttttga gcaacttttt tcgatgaaaa agttgcaaag tgactttata tttgtgctat
     6541 tcaattcttt ttaaacaaca attgacaatg ttatttttca ttgaactgat gttagtatat
     6601 cataaattac ctttaatacg tagatttata gcatattaaa catttttttc aaaaaaagca
     6661 ttgtagtttt ttgtaaatat gaaatttgat taactttgca acgctaaaat ttaaagctat
     6721 tgacccatgg tgtagtggta acacagcaga ttttggttct gttatcctag gttcgagtcc
     6781 tggtgggtca acgcaagcca ttgataaaaa tcagtggctt ttttgtttta tatggtttac
     6841 caccagcttt aagcgtaaat attttccaag ctggttactt agcaacgcga gctgtttcaa
     6901 acgctctatt taggcataaa aaaacacgcc gcagcgtgtt tgtcctatct aacctaagaa
     6961 tcaaataatt aagaaacctt ttttccaacc cttttccttt cgttctcgtt aaggataatt
     7021 ttacgtaaac ggattgattt tggggttact tcaacatatt catcttcttg gatatactct
     7081 aaagcctctt caaggctgaa gcgtaccgga ggggctatct tcatcttttc gtccgaaccg
     7141 gtggcacgca tattggtcag ctttttggtc ttggtaaggt tgaggcaaag gtcgtcagga
     7201 cgtgtatttt cgccaacaac ctggccttcg taaatttctt cgccggggta aacaaagaaa
     7261 attcccctgt cttgaagttt gtctaaggca taagcaatag ctgtccctgt ttccatagct
     7321 ataagcgaac cgttgatacg cctttcaatg ttccctttta aaggttcgaa atctttaaag
     7381 cggtgcgaca taatggcttc acctgtagtt gccgtaagca aattgctgcg caacccaatg
     7441 ataccccgag aaggaatgat aaaatcaata ataacacgct catcccggcg ttccatgttg
     7501 accatgttcc cttttcgttt ggttaccatg tcaatgacag tacccgaaaa ttcatcaggg
     7561 acatcaatag tcaagaactc tacaggttcg catttttcac cgtttatttc ttttatgatt
     7621 acctggggct gtccaacctg gagctcataa ccctcacgac gcatggtttc tataaggatt
     7681 gacaggtgca ataccccgcg gccaaataca acgaacgaat cggccgagtc agtttgttcg
     7741 acacgcaaag caaggttttt ttccaattct ctgtcgagac gttctttgat atggcgtgat
     7801 gtaacaaact taccatcttt accaaaaaaa ggcgagttgt tgatggtaaa cagcatactc
     7861 atggtaggtt cgtcaattga tataggtttc aagccctctg gattgtcata atctgcaacg
     7921 gtgtcgccaa tttcgaagcc ttcaatgcca acgatggcac aaatatcacc agcattaact
     7981 atttctacct ttttgcgctg aagcccttca aacaggtaaa gttctttaat gcggcttttt
     8041 acaatagttt tatcacgttt taccagtgaa atggccatac cttcttttag tgtgccacgt
     8101 tgtaaacggc caatggcaat acggccaacg tacgatgaat aatccagcga agtaatcaac
     8161 atttgtggag taccttcagt aactttaggg gcaggaatgt atttgataat agcttctaat
     8221 aaagccacaa tatctgttgt tggttttcgc caatcattgc tcatccatcc gtttttgccc
     8281 gaaccaaaca aagttgggaa attcaactgg tcttcgtttg cgccaagatg gaacatcagg
     8341 tcaaacactt gttcctgaac ttcgtcaggc ctacagttag gtttgtcaac tttgtttacc
     8401 actacaatag gtttcaatcc catactaagc gctttttgca atacaaagcg ggtttggggc
     8461 atggtgcctt caaaggcatc gaccaagagc agcaccccat cggccatgtt gagcacacgt
     8521 tctacttcgc ccccaaaatc ggcgtggcct ggagtgtcaa tgatattaat tttggtatct
     8581 ttgtatatca ccgaaacgtt ctttgccaaa atggttatac cacgttcgcg ctccaattcg
     8641 ttgctatcca taatcaggtc gcccgggttt tcatgatctt tgaaaatttt tccagccaaa
     8701 atcatcttat ccacaagagt tgtcttaccg tggtcaacgt gagcaataat agcaatgtta
     8761 cgtatatttg tcatcgaaaa aaaatgaagt tttgatacct tacaaaaagc ccgcaaaatt
     8821 aatcaaattt catgaaatcc gggttaaata aatttaattt gtgataatat attttttttc
     8881 aatctgaaaa ttatttttac ctttgccgtt tgtaaaattt tgggtatttg gaaagaagta
     8941 aaaggttgat agtgaatttc aaaggtttgg ctgatggaaa gcatcagttt acttttgaac
     9001 gaaaagatga cttttttgcc gctattgaat actcggaatt tgaaaaaggg gaaatagatg
     9061 ttaaagtaat attggacaaa ggtttcagca atatcacgct ggatgtttct ataaaaggac
     9121 aagtgatggt gatgtgtgac aggtgccttg acccgtatta cgaaaaaata aagtttgacg
     9181 ggacgttgta tgcgaaatat gatgatgccg gtactgatgc agatgaggaa attattttgc
     9241 taggtaaaaa tgactttgaa attgacatca cccattatgt ttatgaatcg gtatgtttga
     9301 gcttgccata ccagcgtgtc cacaaaaaag gtgcatgtaa tgaacaaatg atagagctga
     9361 ttaagaaaca taaaagtaat tgagatatta attttaaaaa tttaagacaa tgccaaatcc
     9421 gaaacacaga cattcgagaa cgagaagaga caagagaagg acacattaca aagcagtagc
     9481 tcctaccact gcaacttgtt cgaattgtgg cgctgcagtt ttataccaca gagtatgccc
     9541 ggaatgtggc tactatcgtg gaaaaatggc cgtagaaaaa gaagtagcag cttaatcata
     9601 cccgcaaaag tatgataaag gtcggtatag acatatctgg aggggatttt gcacctgaag
     9661 caacagtgaa aggtgcaata ttggctcaaa aagagctgcc ttcaaatgtc aagcttgtgt
     9721 gcataggaga caaggctcag attgatgctg tatgtgcacg cgaaggtttt gacaattcgg
     9781 tttgggaaat agtccatact accgaaaaca ttggtatggg cgaacatcct gccaaagcgt
     9841 accaacaaaa agagaatgct accatcccat tgggctttaa acttctgaaa accggcaaaa
     9901 ttgatggttt tgccagtgcc gggagtaccg gggccatgat ggttggcgcc atgatggttg
     9961 ccaaagtggt tcccaatgtt atcaggcctt ctatagctac atttatgcct acagattcag
    10021 atattcctac tttgcttacc gatgtaggca ttaatcctga taccaagcca gatgtgcttt
    10081 tacaatatgg tattcttggg tcgctttacg tgcagcatgt ttttaatata aaaaatcccc
    10141 gggtagcttt acttaacatt ggttcggaag ctgaaaaagg caatttgctt actaaagcag
    10201 cttatccgtt atttaaggaa tgcaaaagca tcaattttgt tggaaatatt gaaggacatg
    10261 acttttattc caatgaaaaa gctgatgttg tagtatgcga tggatttgtt ggtaacgtag
    10321 tcctcaaggg ttcagaagct ttttatgaat tgctgaagaa gagaaatatt catgacccgt
    10381 tctttgaaaa attcaactac gagaatttcg ggggaacccc aattctcggc attgatgctg
    10441 ttgcagttat tggtcatggt atttcaaacg atattgccat aaaaaatatg attttgcaaa
    10501 ctatgcatgt agcacaggca ggcttagtgg aaaaaattaa agaagcattt aactaatgac
    10561 aaaattcaga gcagctatta ctggtattgg agcagctttg cccgattacg tccttacaaa
    10621 tgatgaattg gccactatgg tggaaacttc tgatgagtgg attatgcagc gcatcggtat
    10681 cagggaaaga aggattttga aaggggaagg taaaggtgca tctgatttag gggcagaagc
    10741 agtcaaccaa ttgttccaaa aaaccggatt aaagcctgaa gaagttgaat tagtggtttg
    10801 tgccactatt acaggcgatc atccatttcc ggctacagcc aatataattt gtgataaggt
    10861 agggataaaa aatgcctgga gctttgatat ttctgctgcc tgctcgggtt ttatttatgc
    10921 tttggaaata gctgccaagt ttgttgagtc tggcgaatat aaaaaggtgg ttgtggtcac
    10981 ccccgaaaag atgtcggcta ttaccaatta caaagaccgt acaacctgtc ctttgtttgg
    11041 cgatgctgcc gctgccatat tgcttgagcc tgcaactgag gaagttggag ttatggatta
    11101 tatacttcat gctgatggcg taggaagaca atatttgtat atgaaatctg gaggttcggt
    11161 tagtcctgct tcgcataaat ctgttgacaa tgatgaacat tttgtttacc aggaaggcca
    11221 aaccgtgttc aaatatgctg taacacgtat ggctgatgtg gctgccgaaa tcatggagaa
    11281 aaacaagctc aaccccgata ctacatggtt agtacctcat caggccaata tacgtattat
    11341 tgatgctgta gccagacgaa tgaatgtttc ttctgaaaaa gtgatgataa atattcagaa
    11401 gtatggcaat actactgctg caactattcc attatgcctg tgggaatggg aaaaacaact
    11461 caaaaaaggc gacgaccttg tacttgttgc ttttggtggg gggtttacct ggggctctat
    11521 ttatctcaaa tgggcttacg atagcaaata ataacttatc aagatattaa gaaaaccggc
    11581 attgtccggt ttttttgttt ttaggcaagc ttaggctgtc gtgcgtttgt aacgcttgct
    11641 tagtcaatac ttaggctggc acacgttaca aacgcgcgac agccgcttat atgtgtctgc
    11701 gcttgtgtct ataattcttg cagtttgtaa ctgctttcgg gtgttaaaac taagcgtcta
    11761 agccaaagcc ttgaccaggt gcaataaaat acttatcttt ggtacatggg ccaaaagtac
    11821 atgcatactg ccccgcacaa cggattgccg gccatgcagc cccgtttcga ccgtggctac
    11881 tgtggccagc tagcgcgcgt ttgtaacgcg tgcaatgtta caacaacggg caaaagatga
    11941 attaagcacg aaataaatta agtgtgttat attgttgttt gttgtaaaat ccctgctagc
    12001 gcgtctgtga cgcgtgctgt aacaacagcg gacaaaagat gaagtaagca cgaggtaaat
    12061 taaaggcatt gtatagttgt ttgtaacgcg tgctgttgca acaacgggca aaagctaaag
    12121 taagcagtat ataagtaaaa gcgttgtatt gtcgtttgta acgacgttgt gcaaagtaga
    12181 tagaaaatgc acgcgttaca aacgcgctag ctttagtaaa tgttataagg gtttaaaagt
    12241 ttaatgcctt taatttataa ttctgggatg ggtgaaatcc ctcgccttta ggcgaagcat
    12301 tt
//
LOCUS       DAOSPF010000083        11940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5312_length_11940_cov_8.761548, whole genome shotgun sequence.
ACCESSION   DAOSPF010000083 DAOSPF010000000
VERSION     DAOSPF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_11940_cov_8.761548"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..389)
                     /locus_tag="PK252_12725"
     CDS             complement(30..389)
                     /locus_tag="PK252_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT15616.1"
                     /translation="MGKKYLLDTNTVIDYMGNKLPESAKKELAKIIDDEINLSVINKI
                     ELLGFAKVDQDFIDFVNFSNIYQLDEDITELTIELRKKNRIKLPDAIVAATALYHGFT
                     LVTNNTSDFDKTHVPHA"
     gene            complement(368..589)
                     /locus_tag="PK252_12730"
     CDS             complement(368..589)
                     /locus_tag="PK252_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15617.1"
                     /translation="MIKTVTVEIKNDIALKFLQNLESMHLIKLIEKKPVATKQKLSER
                     FAGCLTKERADELQTELIQMRKEWERNTY"
     gene            complement(1144..1728)
                     /locus_tag="PK252_12735"
     CDS             complement(1144..1728)
                     /locus_tag="PK252_12735"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT15618.1"
                     /translation="MKKLVLLSAIAIVSLAGHSQTKWNVDKVHSNVGFTVQHMVISEV
                     DGSFKVFDGSIVAKNADFTDADINFTVDIKSINTDNEMRDNHLKSADFFDADKYPQMV
                     FKSISFKKLTGNKYELIGTLTIKGVSKQVKLDVTYGGTAKDPYGNIKSGFKVSGIINR
                     FDYGLKWNTLTEAGGAMVGQDVVLNIKLEFTQVK"
     gene            1856..2221
                     /locus_tag="PK252_12740"
     CDS             1856..2221
                     /locus_tag="PK252_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15619.1"
                     /translation="MKSTNDYNCCPVKDILGRIGDKWTLHVVLLLGEKKTLRFNEIKN
                     NVNGISQRMLAVTLRSLEHDGLVKRTLYAGVPPRADYELTPMGLGLYGQFQSLVLWAE
                     QNMEDIMKSRRNFEIDGNQ"
     gene            complement(2215..3006)
                     /locus_tag="PK252_12745"
     CDS             complement(2215..3006)
                     /locus_tag="PK252_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOT15620.1"
                     /translation="MEKTKIYQVDAFTDKLFAGNPAAVCLFDKWPEDKVMQNIAAENN
                     LAETAFVIFENDQYHIRWFTPTVEVDLCGHATLAAGYIIIQHLKKVQKTIVFQSRYRG
                     RLSVTNDNEMLWLDFPTDTLKTCHLENEIMKGTGFMPVETLKGKNDVMAIFQHEDIIK
                     AIKPDFKTISALDARGLIVTAPGNKVDFVSRFFAPQSGINEDPVTGSAHTSLTPYWSA
                     KMGKEKLSAQQLSQRGGKLQCINKGTRTLIGGNATLYLEGDIYIH"
     gene            3121..3849
                     /locus_tag="PK252_12750"
     CDS             3121..3849
                     /locus_tag="PK252_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15621.1"
                     /translation="MRNHIFNGILATIALHLIIGIIFYSAKITGMYVTTVEVKVETPQ
                     TIKEDYEEQLKKMLQEREQLLKMAQRADALIAAQNRKNIGVNVADKTSPAMDKDLQDV
                     QRELEEAKKQIASVQENLERQNEKKIITSDDKEAPSAGLHQEKPQGKLAVYKGPTNIY
                     YDLAGRKTVYLYIPVYKCQSLGKVVVNIVVDASGSVVDAKVDKNQSDPDECLIEAAED
                     AALRSRFSAGEKQKGNITYLFVAQ"
     gene            complement(4040..4162)
                     /locus_tag="PK252_12755"
     CDS             complement(4040..4162)
                     /locus_tag="PK252_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15622.1"
                     /translation="MGNTPYVRGYSEKEALRLNDQAQNIDELIHSWPKTITYAK"
     gene            complement(4229..6061)
                     /locus_tag="PK252_12760"
     CDS             complement(4229..6061)
                     /locus_tag="PK252_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOT15623.1"
                     /translation="MINFRFLLGFYKAAEKIEAQEAALIKEYEELNSYTNSEELARYF
                     ELKKIVESGAFAQKKKDLAALDYKKTEAFAKETAFKNLEKSAQIKNYFKVLSSPAYSN
                     YTTLKDAEKLEKYLKLKEIVKQPEFSNLKESLKKLNFKGSEEFNKEKELASQAKSKLV
                     KNYYSVLQSEAYKNFTHVHDSEDLKKYQELKDKATSDAQAANEFKILSKDPRFIQYYK
                     FLQSPAYALFVEANKNGIIEKYEKLKAEVESDTFKEKKAYLLLPFDKKWEKETQSSQE
                     KEYMQLAADSTIKDYEKFVAGKEFALFNEALKNGTVDKFQELKAFVESEEFKTEKAYM
                     LLKYEQKWRQSEEYQQEEEYKKLAASEKIKWYLSIANSNKFDYLKAWKQTFADEFDTD
                     KLDASKWLTRYYWADSMLNQPYAMFDDQYYPTDGKNLKIDNSILNIETKAEKIQGIGW
                     NPALGFMPKEFNYTTGIINSGKSFRQKYGRFRAKIRLAQGSNVLSAFWMAGDQVLPHL
                     DIAKNIHGKLSFGNIWTKDGSIDKSGESMSSSRFAKAFHIYELEWTPEELIWRINDIE
                     IFRTSSSHLTEPLYLQLGGGVISKNGNAENATMQVDWVRCYQKA"
     gene            6243..6590
                     /locus_tag="PK252_12765"
     CDS             6243..6590
                     /locus_tag="PK252_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT15624.1"
                     /translation="MTQQNENTEVQMRKGVLEYCILLILSKQDAYSSDIIDTLRNNDV
                     NVAEGTLYPLLARLKNNGALQYRWEESKSGPPRKYYHLTAEGAQYLEMLDNSWQRLQA
                     SINNLRELKSINK"
     gene            6603..8108
                     /locus_tag="PK252_12770"
     CDS             6603..8108
                     /locus_tag="PK252_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT15625.1"
                     /translation="MNKAINVNLAGMIFFINEDAYQFLQKYLDEVKSALTGTKPEIDE
                     VMSDVESRLAELLKQRMTGGKEVADMLDVRYATDIIGSPSDFGSGQAGKSNNESGQRQ
                     SIWRKRLYRDADNNVFGGVCSGLAHWLDIDTVLMRILFVILFFVGGSAIPIYIILWII
                     IPKAKTEQQKREMRSGVHEFAGFNEKAKEPTSKSNDFFSFAGDFLKNALKALAKVILV
                     ILGATFAFVGIVIILALILAATVGFSAPFAFDNISLFFYEFSFGPMLAAKFFISLLAI
                     IPAVMLIYAGVLLLMKKRFDRRIGITALLLWFVSLFVVIGLIISNVKNNRQQFTHDNV
                     ALGQTVDGKTIHVSCNENIEPNTKVNVRWFGSHRYDTYFRNDGLVYGTVSLTIEKSPD
                     SAVQILYKKEATADSEEKATKRMRAIEYQFVLQDSSIVFDKYFNLSKGEPFHAQNLEV
                     VLYVPEGKTIVLDNSVDAILKDADVNGSYYISEMAGQSWVMGKNGLTLKSE"
     gene            8490..>11940
                     /gene="gltB"
                     /locus_tag="PK252_12775"
     CDS             8490..>11940
                     /gene="gltB"
                     /locus_tag="PK252_12775"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOT15626.1"
                     /translation="MQIGLPKAQGMYSPDNEHDACGIGFVAHIKGQKSHDIVRRGLEV
                     LINMTHRGAESADNKTGDGAGILIQLPHEFFISKGLKLPSFGKYGAGFLFLPKNENAA
                     KQCLDIFESIVKQENMEIITYRDVPVDSSCLGEIALSSEPAMKQVFVTGKFEQEELER
                     KLFLIRKLAENQVRNSDILDKKAFYFSSFSSKVMIYKGMLMPIQLRDYFPDLTDAAMT
                     SAISMVHSRFSTNTFPTWDLAQPFRYLAHNGEINTIKGNRNWMKAREGLLKTDLFGDD
                     IKKLFPIIEPDKSDSASLDNALEFIHLAGRSLPHALAMLIPESWNSKNPIPESLKAFY
                     EFYSSIMEPWDGPASIVFSDGRFVGGTLDRNGLRPSRYVITKDDIIAMGSEVGCQVFP
                     PESIKEKGRQRPGKILLVDTKLGIIIPDVEIKNQLANMHPYQKWLNESRLILDNIPIR
                     QRVPSELGDELPKYTKVFGYSKEDIEAVITPMATEGYEPTGSMGNDTPLAVLSERPYR
                     LFNYFRQLFAQVTNPPIDSIRESLVMSLTNYIGSVSKNVLVDEPSHCKSIKFKTPIVS
                     NTDLAKLKNLNEENFRHIELPMLFDAKGAKGALQKALEQLCLAAEKAVDDGYNFIVLS
                     DRGINAEKAPIPSLLATAAVHHHLINAKKRMQVGLVIESAEPREVHHFACLIGYGASV
                     INPYGAFAIISDLCAKGKIKHDYVKARELYIKSIDAGLLKIFSKMGISTIRSYHGAQI
                     FEAVGIGDEVINKYFAGTSSRIGGLGFEQLQEEALVYHKEFFIDEVNGDPKTFGFYNY
                     RKTGETHGWDPESIGLLQWATRTNNYDKFKEFTSYVNAHNQKPIFLRGFLDYKKNPIP
                     VEQVEPASEIMKRFITGAMSFGSISKEAHEAMADAMNRIGGKSNTGEGGEDSERFKVQ
                     PDGRNLRSAVKQVASGRFGVTSNYLVNADEIQIKVAQGAKPGEGGQLPGLKVNKIIAR
                     LRHSTPGITLISPPPHHDIYSIEDLKQLIFDLKCANPAAKISVKLVSENGVGTIAAGV
                     AKAKADIIIVSGCEGGTGASPQSSIKHAGLPMEMGIAESHQTLVMNNLRGRVRLQTDG
                     QLKTGKDVVMAAMLGAEEYAFATLALIVLGCVMMRKCHVNTCPVGVATQEEELRKRFT
                     GKSD"
BASE COUNT         3546 a         2183 c         2465 g         3746 t
ORIGIN      
        1 ttacagtccg ttagaacaac ccaccgcctt caggcgtgtg gtacatgagt tttatcgaaa
       61 tctgaggtat tattagtaac cagtgtaaaa ccatgatata gggccgtagc agcaacaatg
      121 gcatcgggta actttattcg gttttttttg cgtaattcaa tagtaagttc tgtaatatct
      181 tcgtctagct ggtaaatgtt ggagaagttt acaaagtcaa taaaatcctg atcaactttt
      241 gcaaagccaa gtagttcaat tttattgata accgaaaggt taatttcatc gtcaataatt
      301 ttagccagtt cttttttagc gctttcgggc agcttattgc ccatgtaatc aataaccgtg
      361 ttggtgtcta ataagtattt ctttcccatt ccttacgcat ttgaattagt tctgtttgca
      421 gctcatcagc gcgttccttt gttaagcaac cggcgaaacg ttcagaaagt ttttgttttg
      481 ttgcaacagg ttttttttct ataagcttaa tgagatgcat actttcaagg ttctgcaaga
      541 atttcaatgc tatatcattt tttatttcga cggtcacagt ttttatcatg atgcaatttt
      601 attaacttgt ttatcccaat atgatctttg tgaaataatt gtaaaattac aataaaaacg
      661 gattggatac aaaaatgatt tcctggctaa ttccagctag cgcgcgtcta tgacacgtgc
      721 tgcatcaaca acgggcaata gctaaagtta gcagtgtgta aagtaaagat gttgctttta
      781 aagaaattgt ataggaatgt agatagaata ggcgcgcgat gcaaacgcgc gccagcattg
      841 ggacactaag ccggacagga ttgttatgtt tttttatata tttataaccc ctttgatgat
      901 ataatcgtat aaaactataa catactttgt ggatatgaac aacgatattt taattatggt
      961 acttgctggc attgtaacgt tgatattgat tgttggtgtt tatggacgtt attttgtaag
     1021 ccctaaaaaa tagttattta tttttttgtc ccacaatcca acctaacgta ctaatggtga
     1081 gcctgcgact tgcattctgt ctatcgtgac ctgccaataa aagcggtttt gttttgcttc
     1141 atgttatttt acctgagtaa attcgagttt tatgttcaac actacatctt gaccaaccat
     1201 ggcgcctccg gcctcggtaa gggtattcca ctttagcccg taatcaaagc ggttgatgat
     1261 ccccgatact ttaaagcctg atttaatatt gccataaggg tctttagctg taccaccata
     1321 ggtaacatct aattttactt gttttgaaac gcctttaata gttaatgtgc ctatcaactc
     1381 gtatttgttg cccgttaatt ttttgaaaga aatactttta aaaaccattt gaggatattt
     1441 gtctgcatca aaaaagtctg ccgatttcag atggttgtcg cgcatttcgt tgtctgtatt
     1501 gatgcttttg atatccactg taaagttgat atcggcatca gtgaaatcag catttttagc
     1561 cacaatagac ccgtcaaaga ctttgaatga gccatctact tctgaaatga ccatgtgttg
     1621 tactgtaaac ccaacattgg agtgaacctt gtccacgttc cactttgttt gagaatgacc
     1681 tgccaatgaa acaatggcaa ttgcggataa taaaactagt tttttcataa ctttcttttt
     1741 tagattattg atattcaatt ttggtatatt ttcataacca ggttattttt gtttactaaa
     1801 cagattaggt tttaatttgt ttagtaagag agaataattt aaaaaaatac atataatgaa
     1861 atcaacaaac gactataatt gctgtccggt aaaagatatt cttggcagga taggtgataa
     1921 atggacgtta catgtagtgc ttcttttagg agaaaaaaag actttgcgtt ttaatgaaat
     1981 caaaaacaac gttaatggga tatctcaacg tatgttggcc gttacattga gatctcttga
     2041 gcacgacggg ctggtcaaac gcactttata tgccggggta cccccgaggg cagactacga
     2101 gctgacccca atgggcttgg ggctatacgg acaatttcag tctttggtgt tatgggccga
     2161 acaaaatatg gaagatatta tgaaaagcag gcgcaatttt gaaatagatg gtaatcaatg
     2221 aatataaata tccccttcca aatacaacgt ggcatttccg ccaatcaaag ttcgtgtgcc
     2281 tttattgatg cactgtagct ttcctccccg ttgtgaaagc tgctgtgctg aaagcttttc
     2341 tttgcccatt tttgctgacc aataaggagt aagactggta tgtgctgacc ctgttacggg
     2401 gtcttcgttg atacccgatt ggggggcaaa aaaacgggat acaaaatcca ccttattgcc
     2461 cggggcagtg acaatcaggc cacgggcatc taatgccgaa atagttttaa aatctggttt
     2521 tatagctttt ataatatctt catgttgaaa aatggccata acatcatttt ttcctttgag
     2581 agtttcaaca ggcataaacc ctgtgccttt cattatttca ttttcaagat ggcatgtttt
     2641 taaagtgtca gtggggaagt cgagccaaag catttcattg tcgtttgtca cactaagtcg
     2701 gcctctatat cgagattgaa aaacaattgt tttttgcact tttttcaaat gctgaattat
     2761 aatatagcct gctgccagtg tggcatgtcc gcacaaatct acttcaaccg taggggtaaa
     2821 ccaacgtata tgatattggt cgttttcaaa aatcacaaaa gctgtttcgg caaggttgtt
     2881 ttcggcagca atattttgca ttactttatc ttcgggccat ttgtcaaata agcaaacagc
     2941 cgcagggttc ccggcaaata acttatcggt aaatgcatcc acttggtaaa tttttgtctt
     3001 ttccatactt taacaatatt tcaccttttc tttgaggtgg caattagttt tagtcaatca
     3061 aatttacaaa aatcattccc tatcccaatc aactttgtat atttgtaaaa aaactgaaga
     3121 ttgagaaacc acattttcaa cggcatacta gctaccattg cacttcacct gattattggc
     3181 attatctttt attcggcaaa aataaccggg atgtatgtta ccactgtaga ggttaaggtt
     3241 gaaactccgc aaaccatcaa agaagattac gaagagcagc tcaaaaaaat gttacaagag
     3301 cgagaacagc tgttgaaaat ggcgcaaagg gccgatgcat tgattgcagc tcaaaacaga
     3361 aagaacattg gcgttaacgt ggccgacaaa acttctcctg ctatggacaa agacttgcaa
     3421 gacgtacaac gcgaattgga agaagctaag aaacaaattg catcggtgca ggaaaatctt
     3481 gaaaggcaaa acgagaaaaa gataatcact tctgacgaca aagaagcccc atctgccggc
     3541 ctacaccagg aaaagcctca ggggaagctt gcagtttata aagggcctac caatatttat
     3601 tacgatctgg ccgggcgaaa gacagtatat ctctatatcc ctgtatataa atgccaaagc
     3661 ttaggcaaag ttgttgtaaa tattgttgtt gatgccagtg gtagtgtggt agatgccaaa
     3721 gttgacaaaa atcagagtga ccctgacgaa tgtcttatag aagctgctga agatgctgcc
     3781 cttcggtcgc gttttagtgc tggcgaaaag caaaaaggca atattaccta tctttttgtg
     3841 gcacagtaat tttaaaccta tcaaatgcag ttttttcttg tttacctgtt gggtttattc
     3901 gctgaagcct ttgtcaaaag tgtttttgtt tatctggcct tttgcaacag cattgtttat
     3961 tatgcctttt ctaaaaataa ggttttgagt cagctacaca tatcaaacgt tgagtaagcg
     4021 gatatttgaa agcccagatt tatttggcat aagttatagt tttaggccaa gaatggataa
     4081 gttcgtcaat gttttgagcc tggtcgttaa gtcgtaaagc ttctttttcg ctataaccgc
     4141 gtacataagg agtgtttccc atatttttta ttttcaaaaa tacagggaaa aacttaacaa
     4201 aaaggggcta ggccatgata aacagtaatt aagccttttg gtaacaacgt acccagtcca
     4261 cttgcatggt agcattttcg gcattgccgt ttttgcttat tacgccacct ccaagttgca
     4321 ggtacaatgg ttccgtgaga tgagaagaag aagtacggaa tatctcaata tcgttgatac
     4381 gccagatgag ctcttctggg gtccactcca gttcgtagat gtgaaaagct ttagcaaagc
     4441 gcgagctgct catactttca ccacttttat ctattgatcc gtcttttgtc cagatatttc
     4501 cgaaagaaag ttttccatga atatttttgg caatgtctaa atgtggcaaa acttggtctc
     4561 ctgccatcca gaatgccgat aatacattgg agccttgtgc caaacggatt ttagcgcgaa
     4621 agcggccata tttttgcctg aaacttttgc cagagttgat aatacccgtg gtgtaattga
     4681 attctttggg cataaaacct aaagcagggt tccagcctat tccctggatt ttttcggcct
     4741 tggtttcaat gtttaaaatg ctattatcga ttttaaggtt tttgccatcg gtggggtaat
     4801 actggtcgtc aaacatggca taaggctggt tgagcatgct gtcggcccaa taatagcgtg
     4861 taagccattt tgaagcatcc agtttatctg tatcaaactc gtcggcaaaa gtttgcttcc
     4921 aggctttgag gtaatcaaac ttgttggaat tggcaatgct gaggtaccat ttgatttttt
     4981 cggaagcagc aagttttttg tattcttctt cctgctgata ttcttcagac tgtctccact
     5041 tttgttcgta tttaaggagc atgtaagctt tttcagtttt gaactcctcg ctttcgacaa
     5101 aagcttttaa ttcttgaaac ttgtcaacag taccattttt aagtgcttca ttaaacaagg
     5161 caaattcttt gccggcaaca aatttttcgt aatcttttat agtactgtca gcagcaagct
     5221 gcatgtattc cttttcctgt gaagattgag tttctttttc ccacttttta tcaaaaggaa
     5281 gaagcaggta agctttcttt tctttgaatg tatcactttc aacttcagct ttgagctttt
     5341 cgtatttttc aataatgcca tttttattgg cctctacaaa taatgcatac gcaggagact
     5401 gaaggaattt ataatactgg ataaaacgag ggtctttgct taaaatcttg aattcgttag
     5461 cagcctgagc atctgaggta gctttgtcct ttaattcttg atattttttg aggtcttcag
     5521 aatcgtgaac atgggtaaag ttcttgtatg cttcgctttg aagcactgag tagtagtttt
     5581 ttaccagttt tgattttgcc tgggaggcta gttctttttc tttattgaat tcttcagagc
     5641 ctttgaaatt aagctttttt aagctttctt ttaaattgga aaactcaggc tgtttgacaa
     5701 tttcttttag ttttaaatat ttttcaagtt tttcagcgtc tttcaatgtg gtgtaattac
     5761 tataagccgg tgagctcaac actttgaaat agttctttat ttgagctgac ttttcaaggt
     5821 ttttaaacgc tgtttcttta gcaaaagctt cagttttttt ataatcgagg gcagcaaggt
     5881 cttttttctt ttgggcaaaa gctccagact cgacaatttt cttcaactca aaatatctgg
     5941 ccagctcttc agaattggta tagctgttaa gttcttcgta ttcttttatc aatgcggctt
     6001 cttgtgcttc aattttttcg gcggctttgt aaaagcccaa tagaaaacga aaatttatca
     6061 taagttaagt atttaatcag tacaaattaa aaatctaaga tataaccttt tcattaaatg
     6121 cacaaattaa gtcgaaaaaa cctgttttta acttattgtc acaaaatatt tataattttt
     6181 agtactatgt attgcatagt actaaaaatt gcattatttt tgactatcaa aattttataa
     6241 aaatgacaca acagaatgaa aatacggaag tacagatgcg caaaggtgtg ctggaatatt
     6301 gcatacttct gatattgtct aaacaggatg catattcgtc agacatcatt gatactttgc
     6361 gcaataatga cgtgaacgtg gcagaaggga cactttaccc tttgttggcg aggctcaaaa
     6421 acaacggggc gttgcaatat aggtgggaag agtcaaaatc ggggccgcct cgcaagtatt
     6481 atcatttgac tgctgaaggg gcacaatatt tggaaatgct tgataatagc tggcagcgtt
     6541 tgcaagcatc gatcaataat ctacgagagt taaaaagtat aaacaaataa ataagaaaaa
     6601 ccatgaataa agctataaac gtaaatttgg caggaatgat ttttttcatc aacgaagatg
     6661 catatcaatt tctgcaaaag tatttggatg aggttaaatc tgctttgaca gggaccaaac
     6721 ctgaaatcga tgaagtaatg tcggatgtcg aaagccgcct tgccgaattg ctcaaacaac
     6781 gtatgaccgg gggtaaagaa gttgctgata tgttggatgt caggtatgcc accgatatta
     6841 ttggctcgcc aagtgacttt ggaagtggcc aggctggcaa gtcaaataac gaaagcggcc
     6901 aacgtcaatc aatttggcgg aagcgtttgt accgtgatgc tgataacaat gtttttgggg
     6961 gagtttgttc ggggttggcc cattggcttg atattgatac cgtattgatg cgtattttgt
     7021 ttgttatcct tttttttgta ggaggctcgg caataccgat atacattatt ttgtggatta
     7081 ttatccccaa agccaaaaca gaacaacaaa agcgcgaaat gcgctcgggg gtgcacgagt
     7141 ttgctggctt caatgaaaaa gctaaagaac ctactagtaa atcaaacgat ttttttagct
     7201 tcgccggtga ctttttaaaa aatgccttaa aagcactggc aaaagttatt ttagtcattt
     7261 taggcgcaac ttttgcattt gttggcattg taattatatt agcgttgatt cttgctgcca
     7321 cagttggttt ttcggcacca tttgcttttg ataatatttc gctgtttttc tacgagtttt
     7381 cttttggccc gatgctggct gccaaatttt tcatctcgtt actggccatt atacctgctg
     7441 taatgcttat atatgccggg gttttattgt tgatgaaaaa aagatttgac aggcgcattg
     7501 gcattaccgc actactgtta tggtttgtat ctttgtttgt ggtaattggc ctgataataa
     7561 gcaatgtaaa aaacaatagg cagcaattta cccatgataa cgttgcttta gggcaaactg
     7621 ttgatggcaa aacaattcac gtttcgtgca atgaaaacat tgaacccaat acaaaagtaa
     7681 atgtccgctg gtttggttca catcgttacg atacctattt cagaaatgat ggcttggttt
     7741 atggaaccgt ttctttaact attgaaaaaa gccctgatag tgctgttcaa atattgtaca
     7801 agaaagaagc aacagccgac agtgaagaaa aagcaacaaa acgtatgcgt gccattgagt
     7861 atcagttcgt ccttcaggat agcagtattg tttttgacaa gtatttcaat ctttccaagg
     7921 gagaaccatt tcatgcacaa aaccttgaag tagtattgta tgtacctgaa ggaaaaacaa
     7981 ttgtgctgga caacagtgtt gatgcaattt tgaaggacgc tgatgtgaac ggttcatact
     8041 acatttcaga gatggcagga caaagctggg tgatgggtaa gaatgggcta accttaaaaa
     8101 gtgaataatt atttattaat aatgtataaa tactcttttt ttatattttt aaaataaaca
     8161 gattttatga tttagctttc atttttttat caaaaagcaa tattttaatg taattattaa
     8221 aaaatttttc ttaaaaattg ttttaatatt aaattttggc tgtatatttg cacacagaaa
     8281 acaaattttg cttatgacta aaaatacatc catatctaag aagaataaga agagcttact
     8341 cgggtgagga tggtatgatt tttagaaaaa gataaaatgt ataaatcggc tgttctcacc
     8401 aaaaggaacg gccgatttgc attataaggc atgtgtataa aattaaaata attagtaatc
     8461 tggttttaaa accaaaaaga aaagtgagta tgcagatagg attgccaaaa gcgcagggga
     8521 tgtacagtcc cgacaatgag catgatgcgt gtggtattgg ttttgtggcc catatcaaag
     8581 gccagaaatc gcatgatatt gtgcgtcgcg ggttagaagt gttgataaac atgacacacc
     8641 ggggggctga aagtgccgac aacaagaccg gggacggagc aggtatttta atccagctcc
     8701 cgcacgaatt ttttatttcc aaaggattga agcttccttc atttgggaag tatggagcag
     8761 gtttcctttt tttacccaag aatgaaaatg ctgcaaaaca atgcctggat atatttgaaa
     8821 gcattgtaaa acaagaaaac atggaaatca tcacttaccg tgatgtgcct gttgacagtt
     8881 catgtcttgg cgaaatagcg ctgagcagcg aacctgctat gaagcaggtt tttgttaccg
     8941 gtaaatttga gcaggaagag ttagaacgta aacttttcct tattcgtaag ctggccgaaa
     9001 accaagttcg gaattctgac atactggata aaaaggcttt ttatttttca agtttttcat
     9061 caaaggtgat gatatataaa gggatgttga tgcctattca attgcgcgac tacttccccg
     9121 acctgacaga tgcagctatg acaagtgcca tatctatggt acactcgcgt tttagtacta
     9181 ataccttccc aacttgggat ttggcgcagc cttttcgcta ccttgcgcac aatggcgaaa
     9241 tcaacaccat caaaggtaac cgcaactgga tgaaagcccg cgaaggcctt ttgaagacag
     9301 acctttttgg ggacgatatc aaaaaattat ttcctatcat agaaccagac aagagtgaca
     9361 gtgcctcatt ggataacgca cttgagttta tccaccttgc aggtcgcagc ttgccacatg
     9421 cattggcaat gctcattcct gagtcatgga actctaaaaa cccaattcct gaaagcttaa
     9481 aagcttttta cgagttttat tcatctatca tggagccatg ggatggacct gcatctatcg
     9541 tatttagtga cgggcgtttt gtaggtggta cattggaccg caacgggctt cgtccttcgc
     9601 gttatgtcat taccaaagat gatatcattg ctatgggttc agaagtaggg tgccaggtat
     9661 tccctcccga atcgataaaa gaaaaaggcc gtcaacgtcc gggcaagatc cttttggtgg
     9721 acaccaaact gggaatcatc atccctgatg ttgaaatcaa gaaccagctg gccaacatgc
     9781 acccttacca aaaatggctt aacgaaagcc gcctgattct tgacaatatt cctattcgtc
     9841 agcgtgtgcc ttcagagttg ggagatgaac ttccaaaata cactaaagta tttggttata
     9901 gcaaagaaga tattgaagct gttattaccc ctatggcaac tgaaggttac gaacctacag
     9961 gttctatggg caatgatact ccgcttgcag tattgagcga aaggccttac aggttgttta
    10021 attatttcag gcagttgttt gcccaggtaa caaaccctcc cattgactct atacgcgaaa
    10081 gccttgtgat gtctttgaca aattatattg gttcggtttc aaaaaatgtt ttggtagatg
    10141 agccttcaca ttgcaaatct attaagttta aaacaccgat tgtttccaat acagatttgg
    10201 ccaaactgaa aaacctgaac gaagaaaact tcaggcatat tgaactgcca atgctttttg
    10261 atgctaaagg ggcaaaaggc gctttacaaa aagcgctaga acaattatgt cttgcggcag
    10321 aaaaagctgt tgatgatggg tacaacttta ttgtactcag tgacaggggg ataaatgcag
    10381 aaaaagcccc cattccatcc ttactggcaa cagcagcagt tcatcaccac cttatcaatg
    10441 ctaaaaagcg tatgcaggta ggtttggtta ttgaaagtgc tgagcctcgc gaggtacatc
    10501 actttgcatg ccttattggt tatggggcaa gcgttatcaa tccttatggc gcttttgcca
    10561 ttatcagcga cctttgcgca aaaggcaaaa tcaagcatga ctatgttaaa gcccgcgagc
    10621 tgtatatcaa atctattgat gctggcttgt taaagatatt ctcaaaaatg ggtatttcta
    10681 ctatccgcag ctatcatggt gcacaaatat ttgaggctgt tggtattggc gatgaagtta
    10741 tcaacaaata ctttgctggc acctcttctc gtattggggg acttggtttt gagcaattac
    10801 aagaagaagc cctggtttac cataaagaat tctttattga tgaggtaaat ggcgacccca
    10861 aaacatttgg tttttataac tatcgtaaaa caggcgaaac tcatggttgg gatccagagt
    10921 caattggttt attacaatgg gcaacccgta caaacaatta tgataaattc aaagaattta
    10981 caagttatgt caatgcccat aaccaaaagc ctatattttt acgtgggttt ctcgattata
    11041 agaaaaatcc gattccggta gaacaagttg aaccagcttc agaaatcatg aagcgtttca
    11101 tcacgggggc tatgtcattt ggttctataa gcaaagaagc acacgaagct atggcagatg
    11161 ccatgaaccg tattggtggc aaatcaaata ccggagaagg gggcgaagac agcgaacgtt
    11221 tcaaggtgca gccagacggg cgcaatctgc gcagcgctgt taagcaggta gcttcgggac
    11281 gttttggtgt aaccagcaat tatcttgtta atgctgacga gatacagatt aaagtggccc
    11341 agggggcaaa acccggtgag gggggccagt tgcccgggtt aaaggtaaat aagataattg
    11401 cccgcttgcg tcactccact cctggtatta cgcttatttc gcctccacca caccacgata
    11461 tctattctat cgaagacttg aaacagctta tcttcgacct gaaatgtgct aacccagctg
    11521 caaaaatcag cgttaagctt gtttcagaaa atggtgttgg tactattgcc gctggtgttg
    11581 ccaaagcaaa agcagatatt atcattgtat caggttgcga aggtggtaca ggagcatcac
    11641 cacaaagttc tatcaaacat gcaggtttac ctatggaaat gggtattgcc gagtcgcacc
    11701 agacattggt aatgaataat cttcgtggtc gtgtaagatt acaaacagac ggacagttaa
    11761 aaacaggaaa agatgtagta atggctgcca tgttgggagc tgaagaatat gcatttgcaa
    11821 cattggccct gattgtactt ggttgcgtaa tgatgcgtaa atgtcacgtt aatacttgcc
    11881 ctgtaggtgt tgctactcag gaagaagaat tgcgtaaacg ctttacagga aaatctgatt
//
LOCUS       DAOSPF010000084        11586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5545_length_11586_cov_7.041453, whole genome shotgun sequence.
ACCESSION   DAOSPF010000084 DAOSPF010000000
VERSION     DAOSPF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5545_length_11586_cov_7.041453"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK252_12780"
     CDS             complement(<1..199)
                     /locus_tag="PK252_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15627.1"
                     /translation="MLFFGLFSTHLPYIVLGILYLVSMGLYSLNQIDSAHFVQKHQDK
                     AEIAIEEPIKADVLKQQSQTTY"
     gene            complement(343..1053)
                     /locus_tag="PK252_12785"
     CDS             complement(343..1053)
                     /locus_tag="PK252_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT15628.1"
                     /translation="MGRAFEYRKERIFKRNASNSKIFTRISKDIAMAVKAAGPDPYSN
                     GRLRMLLQNAKAANMPKDTVDRAIKRASSKEQEDYKEVIYEGYGPHGIAILVETATDN
                     PTRTVSNVRSIFGKFGGSLGNSGSVSFMFEHKCNFKVKNKPGVDLEELELELIDFGVS
                     DVFAEEENIMIYGEFESYGSIQKYLEEKGFEIVSAEFEWIPTDSKELNSEQAAGVMKL
                     IEKLEEDDDVTNVFSNMR"
     gene            complement(1074..1508)
                     /locus_tag="PK252_12790"
     CDS             complement(1074..1508)
                     /locus_tag="PK252_12790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT15629.1"
                     /translation="MKTAEIQTEKGTMKVQFYEEDAPNTVENFIKLSKSGFYDGLTFH
                     RVIPDFVIQGGCPKGTGVGGPGYTIKCETSGGNQYHDRGVLSMAHAGKDTGGSQSFIC
                     HNRRNTQHLDRVHTCFGKVYEGLDVIDAIRPGDKILKITINE"
     gene            complement(1586..2221)
                     /locus_tag="PK252_12795"
     CDS             complement(1586..2221)
                     /locus_tag="PK252_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOT15630.1"
                     /translation="MIKQIAIFGASGRTGRILTGLLLEKGYRVNALVRNPLKFSIEHP
                     NLFVIKGNVNQKEAIEQAIERSEVVVSVLGHVKGSQPLFQTKAVDNIIEVMKARNIKR
                     FVVLTGSGVKMPEDKWSLANSMLTFIIKSIAPARFNDGVEQCKRILATDLDYTIVRAP
                     LLTMQKPKGVYKTGYLKLGFFSHISRYDVAGFICNVIENQIFIKDVPQISY"
     gene            complement(2425..3681)
                     /locus_tag="PK252_12800"
                     /pseudo
     CDS             complement(2425..3681)
                     /locus_tag="PK252_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            4392..5750
                     /locus_tag="PK252_12805"
     CDS             4392..5750
                     /locus_tag="PK252_12805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOT15631.1"
                     /translation="MKNIVTLALFISTLITTQAIGKSNNTACRIDVEIAGLADKKVTL
                     TMHYGLKDIIVDTIKLDSKGRGYLERTKKLPEGIYSVVLPNGKYFELLIGFEQYFTIV
                     SDTSSLVNNISIEGSDETDAFCKYQKLALQLEKVKKDKSNREKIQQSISLLKDSIYKS
                     FDKQYISKYLKMQEEPSLKVDTSKMAKAPIEKFKQLHYLKAVHYFDNIDFSDVRLLNT
                     RLFSEKLDYYFNVFISQQPDTIIQAMDKVMKLAKSNDSTYRFTLSFMWQNFRNIKTPN
                     DELVFVHLAENYYLNNTAPWVDKRFIKILEEKTLSLKKLAIGNIAPDLDLVDIAEKPL
                     SLHKTNADLLLVLFWTPDCDNCTKKMDELNQIYSAYKSKGFKIFAIYTHADKKLWKEY
                     LEQHSYPWIHAYDPLKKSHFEEKYKVEFTPKLILLDKNKNILAKDLSIEQLKKMLLQK
                     SK"
     gene            complement(6022..6357)
                     /locus_tag="PK252_12810"
     CDS             complement(6022..6357)
                     /locus_tag="PK252_12810"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HOT15632.1"
                     /translation="MIEFQMTDWSAIPQTVHKGEVGEAFWKTIQYEGLRVRMVECSPG
                     YIADHWCTKGHIIYCIEGEMVNMLSTGEEQVMKAGMTYQVSDNASSHKTRTDKGVKLF
                     IIDGDFLAK"
     gene            complement(6361..6561)
                     /locus_tag="PK252_12815"
     CDS             complement(6361..6561)
                     /locus_tag="PK252_12815"
                     /inference="COORDINATES: protein motif:HMM:NF022654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HOT15633.1"
                     /translation="MEATELNIDVVYEAPYWVVLFEKIMNNRRMVAKKRIGKNEPRQT
                     ELSKFFESLNYKRLRYSVAPIK"
     gene            6859..9840
                     /locus_tag="PK252_12820"
     CDS             6859..9840
                     /locus_tag="PK252_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT15634.1"
                     /translation="MVKLSNLLFFTLLFISISSLSANKLDSLMLLAGQSGDKEKPALY
                     LQIAKQYQSNNDSLSIDYSIKAVKGAKKFNLQLIEAQALENMGVSYCNQARYQIALDT
                     LMSALKIYEKLNFPQEATLCLNNIGNTYDYLSNFENAEKYYNKALGLAKRYKNRYNES
                     LVVFNLGNIYLKQYKYSKALENFLVSLAIEEQMGNEIGVAYCLNDLAMIYEVLHLYDR
                     QENSIKRALEIFKKKGYVFEESVLLNNLGDFYLSQKFYDKAIENYFLSAKTKESINHV
                     GGLILTYSKIGDVYFTQNITDSAELYYQKAYNIAQKTGFNVNKSSVLLGMGKIHFNKK
                     QYNKAKNAFEECYEIATKGEMNELRRDVLLELYKTDSALGDVAGSNRALRKAYELAQA
                     LNDKEAKQRIGEMMFTFETEKKDREIALQKSINNKQRMLLWAAIVFIVFVTFLSVIIF
                     IQFRNIKKNNQLLALQKAEIDAKNIKLRELDELKSRFFTNISHEFRTPLTLVTGNLEL
                     LHDKAESSGEDVETYNIMLRHSRRLLDLINQLLEISKLEKNKMKLFIHYGNVGEFVFR
                     IVSSFESWAVEKNLIFDYTQIDKSGNCWFDADKLEKVITNLISNAIKYTTEGSITIKS
                     KITGNQFMVEITDTGPGLTTNELGHIWELFYQAGAQADKLYEGSGIGLALVKELVKLM
                     QGEISVSSQPGKGTSFSITLPVDYEQSENIVIVENAESKTYEPIVANENTHKKGLHSG
                     PVQNTNSEMVLVIEDNDDLRHFICSNLLANYRIIEAVDGIDGLEKAFVSVPDLIITDV
                     MMPRMNGLEMTKKIKTDERTSHIPVIMLTARVAHEHVIEGLEMGTDEYLPKPFSIKEL
                     LLRINNILRTKQQIREKLKVSNDLYAPDVHISSVDERFLKKALDIIEAHIEDCDYEVI
                     QFCADLGMSRTNLHRKLKAITGQSTTEFIRNTRLKRAALLLKNNAGTVSEIAYQTGFN
                     NLSYFTKSFKDMYGVTPSEYS"
     gene            9995..>11586
                     /locus_tag="PK252_12825"
     CDS             9995..>11586
                     /locus_tag="PK252_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOT15635.1"
                     /translation="MRKQLLLFLIALPLGILVNAQNLILYYPFDGNANDASGNLNHAT
                     LHGCSFAPDRMGQADKALSFNGTSDFVVTPYRQANITALSYSFWIKIPDNAGEMTIFQ
                     TRGPNNEPGKSITVTLNNGFVRFIYDAAYNLCGKITRQPLEANKWLHVACVWNGTSGQ
                     PVNNNQFSIYINGTQIAANDDNAGSNRPVPPISGDSTMFIGYHKQWNRWFKGSLDDVR
                     VYEGVLSPADISGLYYEKIPLEIVAPQKGKHFIKNTQVEISWVKKAEVPSVNLQYSIN
                     GGAWQNIALNQTGTSYTWTLPNTVASNVKVAAISTSDNTIRSELDGISIAADSAMLGL
                     MLYYPLDGNVQDATLFGNHGQLQGGTYTEDNIMSSNSALAIAGDGQGFRQQVAKSFTG
                     TEGTISMIVYKDNWATADHGIFFLNYPATTSHQMYLNYHDVGLHFRYGDVSLHGNTYL
                     AWNGCFSWKPKTWHHVAITWEQTSGVTMLKMYADGAKVAETTANLMLNDSANWKFAQK
                     IPGSTYGNFNGSMDEVKWYGRKL"
BASE COUNT         3782 a         2264 c         2147 g         3393 t
ORIGIN      
        1 aataagttgt ctggctttgt tgttttaaaa catcagcttt gatgggttct tcaatggcaa
       61 tctcagcctt atcctgatgc ttttgtacaa aatgagcact atcgatttgg tttaatgaat
      121 acaagcccat gcttactagg taaagaatac ccagcacgat gtaaggcaaa tgagtcgaaa
      181 ataaaccaaa aaacagcatt tagcgtaatt ttcagatgaa caccagataa agcatttttg
      241 ttggcacaaa ggtaagtatt tttaaaacaa aaaaactccg aagttcatca cttcagagtt
      301 tctcttttat cttgtttctt gactctttgt tcttgattct ttttacctca tatttgaaaa
      361 aacattggta acatcatcat cttcttcaag tttttcaata agtttcataa cgcctgctgc
      421 ttgttcagag tttaattctt ttgaatctgt agggatccac tcaaattcgg cactaacaat
      481 ttcaaaacct ttttcttcaa ggtatttctg aatgctgcca taagactcaa attcgccata
      541 aatcataata ttttcctctt cggcaaatac atcagaaacc ccaaaatcaa tcaactcaag
      601 ttcaagctct tcaaggtcaa caccaggttt atttttaacc ttaaagttac atttgtgctc
      661 aaacataaaa gatacactac cagaattgcc aagagaacca ccaaacttac caaaaatact
      721 gcgaacattg cttacagtgc gtgtaggatt gtcagtagct gtttctacaa gtatagcaat
      781 accatgaggc ccgtatcctt catatattac ttctttatag tcctcctgtt ctttagatga
      841 agcccgctta atagccctat ctactgtatc tttaggcatg ttggcagctt tagcattttg
      901 cagcaacatt ctaagcctgc cgttagaata aggatcaggg ccagccgcct taacagccat
      961 ggcaatatct ttacttatgc gtgtaaatat ctttgaatta ctggcatttc ttttaaaaat
     1021 tctttctttt ctatattcaa acgcgcgtcc catattatct gtattgttta ttcttattcg
     1081 ttgatagtta ttttcaaaat tttgtcaccg ggcctgatgg catctatcac atccaaacct
     1141 tcgtaaacct taccaaaaca ggtatgtaca cggtcaaggt gttgagtatt gcgcctgtta
     1201 tggcagataa aagattgcga accgcctgta tcttttccgg catgggccat tgacaataca
     1261 cccctatcgt gatactggtt tccacctgat gtctcgcatt taatagtata accaggccca
     1321 ccaacaccag tacctttagg gcatccaccc tggataacga agtctgggat taccctgtgg
     1381 aacgtgaggc catcataaaa gcctgatttg gataatttaa tgaagttctc tacggtattt
     1441 ggagcatctt cttcgtaaaa ctggaccttc attgtccctt tttcagtctg tatctcagct
     1501 gttttcatat ttatatttta attcttgatt cttgtatctt ttttaaaaag cgacaaaatt
     1561 acaattttta tccttcttgc caaaactaat aacttatttg tggaacatct ttaataaaga
     1621 tttgattttc aataacatta caaataaaac ctgcaacatc atatctggag atatggctaa
     1681 aaaagccaag cttcaaataa ccagttttat aaactccttt aggtttctgc atggttaaaa
     1741 gcggtgctcg tacaatggta tagtctaaat cagttgctaa aattcttttg cactgctcaa
     1801 caccatcatt aaagcgagcc ggggcaattg attttattat aaacgtgagc atactattgg
     1861 ctaaagacca tttatcctct ggcattttta caccagaacc tgtcaaaaca acaaatcttt
     1921 taatatttct ggctttcata acctctatga tattatcaac tgccttggtt tgaaataatg
     1981 gctgcgaacc ttttacatgt cctaaaaccg atacaacaac ttcagacctt tcaattgctt
     2041 gttcaatggc ttctttctgg ttaacatttc cttttataac aaataaatta ggatgttcaa
     2101 tgctgaattt taacggattc cttaccaatg cattaacccg ataacctttt tcaagcaaca
     2161 agcctgtcaa tattcttcct gtgcgcccgg atgccccgaa aatggcaatt tgttttatca
     2221 taaaaaattt tattttttaa ggattgaagc ttgcacacac caacaaatat cattgtgtat
     2281 attcactttg ttgataaaag aacttcgcat aattgcgatt tgtataatta agtgcagagt
     2341 tatataaaaa acgcattgct tttttcaatg gcaatgcgtt atctgatgtt gtaaaacaaa
     2401 ttatttgcgt ttttctatac gggcgcggag ctcgttttca taagctttcc agtcagaaat
     2461 tgggttcctg gcaacaccac tgtcaatagc agctttagca acagctggag ctacaactgt
     2521 caacaggcgt gggtcaagtg gcttaggaat gagatattcg cgtccaaagc tcatgcttgt
     2581 tacgttataa gctttcatca ctgactctgg cactggttct ttagtaagtt ttgcaatggc
     2641 caaagtagct gcaaccttca tttcctcatt aattttgttg gcccttacat ctaaggcccc
     2701 tctgaaaatg taaggaaaac ctaatacgtt attaacctgg ttaggataat cgctacggcc
     2761 ggtggcaaaa atgatgtcag gacgtgaagc cttagctttt gcataagaaa tttccgggtt
     2821 tggattagcc aaagcaaata caatagggtc tttagccata ctacgaacca tatcttcaga
     2881 aactacgtct gctacagaaa ggccaagaaa tacatctgca tctttcatag cctcttcaag
     2941 agtgttgata ttacgagttg ttgcaaatgg cagttttcgt ttgttgaggt cggtacgacg
     3001 ggtagaaata acgcctttgc tatctaacat aacaatgttt tccagttttg cccctaaatg
     3061 catatatagg cttgcacatg atgaagcagc tgcaccagca ccgtttacaa cgattttaat
     3121 atcttcaatc tttttgccaa taagctcgag tgcattaata agcgctgcac cggaaataat
     3181 tgctgttccg tgctggtcat catgcatcag tgggatatca agctcttctt ttaggcggtc
     3241 ttcaatatca aagcattcag gggctttaat atcttcaagg ttgataccac caaaagttgg
     3301 agcaatagct tttacggtag caataaattt ttcagtatca gtttcattaa tttcgatatc
     3361 gaaaacgtcg atatcggcaa aaattttgaa caacaagcct ttgccttcca taacaggctt
     3421 tccggcagct gcgccaatgt taccaaggcc taatactgca gtaccattac taataacggc
     3481 cactaagttc cctttagcag tatatttata tacatcgtcg gggtttgctg caatcttaag
     3541 acatggctca gctacacccg gcgaataagc taacgctaaa tctgattgtg tactatgtgg
     3601 cttagtggga ataacttcta ttttcccggg acgtccttgt gcgtgaaaac gcaatgcttc
     3661 ttcttttaaa ttatttgaca tgataattgt attaaaaatt ttgaaggtgc aaatgtaatt
     3721 ttttttaagt gaaatatctt tttgacaaac tttaataagt taaaaactcg ataaaataaa
     3781 caaatattca aatctatttc attaaatcct ttaagatcgc gacataagtg catataggct
     3841 atcctgtgcc ctatataagc ccaatattat tgaataatct atagctctta aatagtaaga
     3901 aactaaaaaa ttgattttcg aatatttttc caaaaaatgc taaagctata attgaaaggt
     3961 atttgattat ttggcaggtt ttaatattat tttaaagcat tcatcctgag tataaaatga
     4021 caaaagcgtt tgaattctat aaaagcatac gttgattatt tcatcatact aaaatttaat
     4081 ttttaaggaa tgatggaaca cccctgttcg ctattttaat actaaagtgt ttatgttttc
     4141 caatcagggg tatttcataa atttaaaatt ttaatggaat gaccaaacac ccttgttgac
     4201 attttattac tttcgtgttt gttcaattcg ttcatgaggg cgctttgcta agttgccctt
     4261 gcaagaatat aattgataac agatactaag aaacaagcat gattcgttaa atacaaacaa
     4321 ggatgaaatc cggttagtcg ggacggggtc tccttcggaa agtccttcag gatataaaaa
     4381 attatttaaa gatgaaaaat attgttacac tggcattgtt tatatcaaca ctaattacaa
     4441 cacaggcaat aggaaaatcc aacaacactg cctgccgtat tgatgttgaa atagccggcc
     4501 tggctgataa aaaagtcacg ctaaccatgc attacgggct gaaagacatt attgtggaca
     4561 caataaagct tgatagtaag ggcagaggat acctggaacg tacaaagaag ctgccagagg
     4621 ggatatacag cgtggtacta ccgaatggca aatattttga gctattgata ggttttgaac
     4681 agtacttcac cattgtttcg gacacttcgt cattggtaaa taacataagc attgaaggca
     4741 gtgatgaaac cgatgctttt tgcaaatacc aaaagctggc cctgcaactg gagaaagtga
     4801 aaaaagacaa aagcaataga gaaaagatac aacaaagtat aagcttatta aaggatagca
     4861 tatataaaag ttttgacaaa caatacatat caaagtatct taaaatgcag gaagaaccat
     4921 cattgaaagt tgatacttca aaaatggcta aggcgccgat tgaaaagttc aagcaactgc
     4981 actatttaaa agctgttcat tactttgaca atatagactt cagcgatgtc aggctgttaa
     5041 atacaaggct cttcagcgaa aaacttgatt attactttaa cgtgtttatt tcgcaacagc
     5101 ccgataccat tatccaagcc atggataaag tgatgaagct ggccaaaagc aacgattcta
     5161 cgtatcgttt tacgcttagc tttatgtggc agaacttccg taatatcaaa acgcctaacg
     5221 atgagcttgt ttttgtccat ttggccgaaa actactatct gaacaacacg gctccttggg
     5281 tagataagag gtttatcaaa atactcgaag aaaaaacctt gtcattaaag aaactggcca
     5341 taggcaacat tgctcctgac ttagatttag tggacattgc tgaaaagcct ttgtcgttac
     5401 acaaaacaaa tgctgacttg ctattagtgt tattctggac cccagactgt gacaactgta
     5461 caaaaaaaat ggatgagcta aaccagatat attctgccta taagtctaaa ggctttaaaa
     5521 tttttgccat atacacccat gccgataaga aactttggaa agaatacctc gaacagcata
     5581 gttatccttg gattcatgct tacgacccac tcaaaaaaag ccattttgaa gaaaaataca
     5641 aagtagaatt tacgccaaag cttattttat tggataaaaa taaaaatata ttggcgaaag
     5701 acctttcaat agagcaattg aaaaagatgc tgttgcaaaa gagcaaataa tacttatgat
     5761 tgctaaaagc cagttcctct taagaacaac cctaagataa acaaatacag ttatttatct
     5821 tctttccttt tttccttgat gaactgagct cacgagcgca aagcgagtaa aaaaggaaac
     5881 gctctcgcct caggcgagat cacgttctta tgaaggtcag tattggcagt agctttaaac
     5941 caatggctac ggagcagaca aaaagaaaga aacgatataa ttgaatatct gattgttata
     6001 gaaaaaatta tcaaattcag tttacttagc aagaaaatcg ccatcaataa taaaaagttt
     6061 tacgccttta tctgtcctgg ttttgtgaga gctggcattg tccgaaacct ggtaagtcat
     6121 gcccgctttc attacctgtt cttcaccggt cgagagcatg ttgaccattt caccttcaat
     6181 acaataaatg atatggcctt tagtacacca atggtctgca atatagccgg gagaacactc
     6241 caccatgcgt acccgcaagc cttcatactg tatagtcttc cagaaagcct cccctacttc
     6301 acctttatga actgtttgag ggattgcaga ccaatcggtc atttgaaatt caatcataac
     6361 ttatttaatt ggagccacag aatatctgag gcgtttataa ttaagacttt cgaagaattt
     6421 tgataattct gtctggcgtg gttcgttttt gccaatacgc tttttggcca ccatacgccg
     6481 gttattcata attttttcaa agagtacaac ccagtaaggg gcttcgtaaa ccacatcgat
     6541 gtttaattca gtagcttcca tatatttaaa atttaatgtt attcaaaact atggttaaac
     6601 aaaaagaatg ccaagtttaa atcggtgaat gacagttttt tgaagctcaa tagttgagaa
     6661 tggtaaataa atacatttcg gcaggcaaga aattgttgtt ttgtagctta aactttgcag
     6721 tttttcacat tgcccatata tgttgaaatg caaataaacc atattgttta gcaaaaaaat
     6781 atctcatcaa agattaattt gaataaaaat tttaaacagt tttttatttt tatgtaataa
     6841 ttataaacaa gctgcgaaat ggtcaaatta tcaaatcttc ttttttttac attgctgttt
     6901 atttctattt caagcctttc ggcaaataaa ttggacagtt tgatgttgtt ggccgggcaa
     6961 tcgggcgata aagaaaaacc cgcattgtac ctgcaaatag ctaaacaata ccaaagcaac
     7021 aatgattcat taagcattga ttattcaata aaagcagtaa aaggggcaaa aaagtttaat
     7081 ctgcaattga ttgaagccca agccttagaa aatatgggtg taagctattg caaccaggcg
     7141 cgttaccaaa ttgcattgga tacattaatg agtgcgctga aaatttatga aaagcttaat
     7201 ttccctcagg aagcaacgct ttgtctgaat aacattggca atacatacga ttacttgagc
     7261 aattttgaaa atgccgaaaa atattacaat aaagcgctcg gccttgctaa aagatacaaa
     7321 aaccgatata acgaatcttt agttgtattt aacctgggca atatttacct taagcagtat
     7381 aaatacagca aagcgctgga aaacttctta gtttcgctgg ccatagaaga acagatgggc
     7441 aatgaaatag gagtggccta ctgcctcaac gacctggcaa tgatttatga agttttgcat
     7501 ttgtacgacc gacaagaaaa tagtataaag cgggcgcttg aaatttttaa aaagaaaggg
     7561 tatgtttttg aagaaagtgt tttgcttaac aacctgggcg acttttacct aagccaaaag
     7621 ttttatgaca aagccataga aaattatttt ctttctgcta aaaccaaaga aagcatcaac
     7681 catgtagggg gcttaatact tacctattcg aaaattggcg atgtttattt tacacaaaat
     7741 ataaccgatt cggctgagct ctattatcaa aaggcataca atattgccca aaaaacaggg
     7801 ttcaatgtta acaagtcgtc ggtgttatta gggatgggca aaatccactt taacaaaaaa
     7861 caatacaata aagcaaaaaa tgcatttgaa gaatgctatg aaattgccac caaaggcgag
     7921 atgaacgaat tgcggcgcga tgtattgtta gaattgtaca aaaccgattc ggcgctgggc
     7981 gacgtagcag gctcgaacag ggcattgcga aaagcttatg aactggccca ggccctcaac
     8041 gacaaggagg caaaacaaag gattggagaa atgatgttta ctttcgaaac cgaaaagaaa
     8101 gatagagaaa ttgccctgca aaaatcgatc aacaacaaac aaagaatgtt gctttgggct
     8161 gccattgtat ttattgtttt tgtaaccttc ctgagtgtta ttatttttat acaattccgc
     8221 aatattaaaa aaaacaacca attgcttgcc ttgcaaaaag ccgagattga tgctaaaaac
     8281 ataaaactca gggagctgga cgaattaaag agccgattct ttacgaatat ttctcacgag
     8341 ttccgcacac ctttgacgct ggttaccgga aacctcgagt tgctccacga caaagcagaa
     8401 agctccggtg aagacgttga aacatataac atcatgcttc gccattcgcg ccgtttgctc
     8461 gatttgatca accagctgct ggaaatctca aagctcgaaa aaaacaagat gaaactcttt
     8521 attcactatg gcaatgttgg cgaatttgtt ttcaggatcg tttcatcgtt cgaatcgtgg
     8581 gccgttgaga aaaacctgat tttcgattat actcagattg acaaaagtgg taattgttgg
     8641 ttcgatgccg ataaactgga gaaagtaatt accaacctta tatctaatgc cataaaatat
     8701 acaacagaag gcagtattac tataaaatcg aaaattaccg gcaatcaatt tatggttgaa
     8761 ataaccgata ccgggccggg attaaccaca aatgaactgg gacatatatg ggaactattt
     8821 taccaggccg gggcccaggc cgataaatta tacgaaggga gcggcatagg gcttgccctt
     8881 gtaaaagaat tggtaaaact gatgcagggt gaaatatctg tgtcaagcca accgggcaag
     8941 ggtacttcat tctcaattac actgcctgtt gactatgagc agtctgaaaa cattgttatt
     9001 gttgaaaatg ccgaaagcaa aacctatgaa ccaattgttg caaatgaaaa cactcacaag
     9061 aaagggctac attcgggccc tgtccaaaat acaaattcgg aaatggtttt ggtaattgaa
     9121 gacaatgatg acctccgcca tttcatttgc agcaacctgc tggccaatta caggatcatc
     9181 gaagcagtag atggtatcga cgggttggag aaagcttttg tttctgtacc cgatttaatt
     9241 attaccgatg tgatgatgcc ccggatgaat ggcttagaaa tgactaaaaa aataaaaacc
     9301 gatgaacgta cttcgcatat cccggtaatt atgcttacag caagggttgc acacgaacat
     9361 gtgattgaag ggctggagat gggtacagat gaatacttgc ctaaaccatt cagcataaaa
     9421 gaattgttac tgaggataaa taatatttta aggacgaaac aacaaataag agaaaaactt
     9481 aaagtctcaa acgatttata tgcaccagat gtccacatca gttctgttga tgaacgtttt
     9541 ctgaaaaagg ctttagatat tatagaagca catatagaag actgtgatta cgaagtgatc
     9601 caattttgtg ccgacctggg catgagccga accaacctgc atcggaaatt gaaagcaata
     9661 accggacaat cgactacaga atttatcaga aatacccgcc ttaaaagggc cgcccttcta
     9721 ctaaaaaaca atgccggaac ggtttctgaa attgcctatc aaacagggtt taacaatctt
     9781 tcctatttta ccaaaagttt caaagacatg tatggcgtta ccccttcgga atatagttaa
     9841 gcttcatttt atcaaaaaaa agaagttgta tttttctgat aatcaatagc tgaaacatca
     9901 ttgtcaaaat ctggaacatc aacgataata ttttaaatag caattcattt tctttgagtt
     9961 aaagaataat aaaatctgat taaactttaa taacatgaga aaacaattac ttctttttct
    10021 aattgcttta ccattaggca tcctggtaaa tgcgcaaaat ttaatactct attacccctt
    10081 cgatggcaac gccaacgatg caagcggcaa cctcaaccat gctacccttc acgggtgcag
    10141 ttttgccccc gacaggatgg gtcaggccga taaagcatta agttttaacg gcacatccga
    10201 ctttgtggtt acaccttatc ggcaagccaa tattacggca ctaagctact ctttttggat
    10261 aaaaatcccc gacaatgcag gcgaaatgac aatttttcaa accagggggc ccaataatga
    10321 accaggcaaa agtataactg taacattgaa taatggcttc gtgaggttta tatacgatgc
    10381 agcatataat ctttgtggca aaataacccg gcaacctctc gaagccaata aatggctaca
    10441 tgtagcatgt gtatggaacg gcacatcggg tcagcctgtc aacaacaacc agttttcaat
    10501 ttatattaac ggaacacaaa tagctgccaa cgatgataat gctggttcaa acaggccggt
    10561 acctccaatt agtggcgata gtaccatgtt tataggttat cacaaacaat ggaacagatg
    10621 gtttaaaggc tcccttgatg acgtaagggt ttacgaagga gtacttagcc cggccgacat
    10681 ttcaggcttg tattacgaaa aaatacctct cgaaattgtt gccccacaaa aaggaaaaca
    10741 ttttattaaa aatacccagg ttgaaataag ttgggttaaa aaggctgaag tgccttcggt
    10801 aaacctccaa tattcgataa acgggggcgc ttggcagaac atagccctaa accagacagg
    10861 gacaagctat acctggactt tgcccaatac cgtggcatca aatgtaaaag tggcggcaat
    10921 atcaacatcc gacaatacca tccgcagcga acttgacggt ataagcatcg ctgccgactc
    10981 ggccatgttg gggctgatgc tctattatcc attggacggc aacgtgcagg acgccacctt
    11041 gtttggcaat catggccaat tgcagggtgg tacttatact gaagacaata ttatgagcag
    11101 caattcggcc ctggccatag ctggcgatgg acaaggcttt aggcaacaag tggccaaatc
    11161 ctttaccggc actgaaggta ccatctcgat gatcgtttac aaggacaatt gggcaaccgc
    11221 cgaccatgga atatttttcc tcaattaccc ggctaccact tcccatcaga tgtacctcaa
    11281 ctaccacgat gttgggctgc atttccgcta tggcgatgta agcctgcatg gaaataccta
    11341 ccttgcatgg aacggttgct tttcatggaa gccaaaaaca tggcaccacg ttgccattac
    11401 ctgggagcaa acctcagggg ttactatgct aaaaatgtat gccgacgggg ccaaggtagc
    11461 cgaaaccact gccaacctga tgcttaacga tagcgccaac tggaaatttg cccagaaaat
    11521 acccggctcg acttatggaa actttaatgg ctcaatggat gaggtaaaat ggtatggacg
    11581 aaagct
//
LOCUS       DAOSPF010000085        11312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5730_length_11312_cov_6.433153, whole genome shotgun sequence.
ACCESSION   DAOSPF010000085 DAOSPF010000000
VERSION     DAOSPF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5730_length_11312_cov_6.433153"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2118
                     /locus_tag="PK252_12830"
     CDS             64..2118
                     /locus_tag="PK252_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOT15636.1"
                     /translation="MRTNTKKLTLVLAVMTLSLRLFGWEGMPMPALHVEGRYLKDASG
                     HIVNLHGFAQTYSPWFNEQGKYWTNYDVAGCLDYNKGIIDGILDAGWKVNFIRLHMDP
                     YWSNTPGVAVTGEADISAFDFTRFKKYLDEVFIPMAEYAISKGLYVVMRPPGVCPEKI
                     AVNDTYNKYLIKVWGYVARHAKLKNNPYIMFELANEPVNILGPDGTYGSGSQGHFDNL
                     KTYFQAVVDTIRASANNIIWAPGLGYQALYQGYAVNPIEGDNIGYAVHVYPGWFNSGQ
                     GYANFQKGWDTQVQPVADFAPILVTEMDWAPARHNKSWGKDSTGVAGKNGFGANFKKI
                     VDDCGNVGWLLFTSPELLAQYGDVTAPADIKDFLTDPEACPKPVYQWYQAYASDYGLS
                     GASESYLTVTGFVVESGKNMTILTKGSAGLKINAVFADGHIENVSSLAKFSVDKTEVV
                     NVARGRVFALADGQATVGILYTDPKGNAWRDTLHVSSTTFPLTNQLFNPDIWGDGTFD
                     EATKTLTTGQWGFGGWTYSNGLDISGYKYLVAKLGSKNNAGVSFRVFDENNYWAGAAE
                     YDFGNKQIVVANTGWMPKKDKSATLNPSHIYIVGFWSNGNIPFVIDTVFLSNSSEYDA
                     LASVKNETTNKNNEPELVDVYNLLGVKIMSQVKKEQAIRELNKGVYLVGKEKVVITRN
                     RK"
     gene            2152..4572
                     /locus_tag="PK252_12835"
     CDS             2152..4572
                     /locus_tag="PK252_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT15637.1"
                     /translation="MKLFRNYLAMLMLFAIGIAGAAQTFQKTDVGVKAKAGSVNVEVQ
                     FYTSDIVRVVKYPEGKAFEKQSLSVVLAPQKISFGVKQQGDELVLTSRKMQVSLNLKS
                     GKVIFSDKSGTLLNEKDAGAAFADFNDAGNKTYTVSQAFALDKDEAIYGLGQQQRGKM
                     SQRNVKLHMVQNNTEDFIPFFVSSKGYGVFWDNYSPTVFEDNTEGTSFKSDVGDGIDY
                     YFMAGNNADGVIACMRQLTGQVPMFPLWTFGFWQSRERYKSQDELTEVVRKFRELNVP
                     IDGIIQDWQYWGNNYLWNAMEFLNPGFYDPQKMVNDVHNMNAHIIISIWSSFGPMTKQ
                     YKELDKIKALYNIETWPQSGSEKWPPNMDYPSGVRVYDAYNPEARDIYWDHLNKGIFS
                     LGMDGWWMDSTEPDHFSWKSEDMDTKTYLGSFRKVRNAYPLMTVGGVYQHQRAVSSDK
                     RVFILTRSAFAGQQRYGANTWSGDVVASWDALRNQISAGLNFSLCGIPYWNSDLGGFF
                     LWNFKNPLENADYRELHVRWIQFGAFCPMMRSHGEGSPRELYQFGKKGNATYDAIEKY
                     IKLRYSLLPYIYSSSWEVTNKQSTMMRALVMDFAADRQALDINDEFLFGKSLLVSPVT
                     QPLYLKNEVRGKDTVKVTDFSTVKSKAVYLPKGADWVDFWTGDKFSGGQTVNKEVPLD
                     IIPVFVKAGSILPIGPEVQYSGEKKWDNLEIRVYEGANGEFTLYEDEGDNYNYEKGAY
                     STVSFSWNNQKRTLSIGERKGSFPGMLTNRVFNIVLVGKGKGTGSVLSAKFDKAITYS
                     GKKQVVKF"
     gene            4783..6381
                     /locus_tag="PK252_12840"
     CDS             4783..6381
                     /locus_tag="PK252_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOT15638.1"
                     /translation="MKKNIIFLLLIAVGVSFYAQKAPEKESTPYTNPVMWADVPDMSI
                     VRTGSDFYLISTTMHLMPGAPVMRSKDLVNWEIVSYVFDSITDNSKYNLLNGTVYGRG
                     QWASSIRYHNGKYYVLFSPNDQPFRSYVYETADPAKGNWKLLARMQHFHDNSLFFDDD
                     NRVYVFSGTGSLRELKSDLSDVLPGGVDMQIFQRDSTENGLLEGSQAFKYNGKYYLLM
                     ISWPRGKPRRQVCYRADNITGPYEKKVILQDNFAGFPYCGQGCITDDGKGNWFALIFQ
                     DRGGVGRVPLLMPVRWVDGWPMLGDENGKVPLTGFTPLKTYNSGNRIVESDDFSGKKL
                     KLQWQWNHNPVNSAWSLTERKGYMRLKTSRVVENIYLAPNTITQRMEGPKCSGVVSFD
                     ISKMKDGDVAGFSAFNGNAALLSIVVLGNKKFLTMSTNVVELDNDSKAVLKVDVEEKA
                     RVELSKDVIFLRIDADFNLNKDLATFYYSFDNKNWTPIGAEFKMIFDYRKFFMGTKFA
                     LFNYATKTIGGYVDVDFFNYNRIQ"
     gene            6549..10685
                     /locus_tag="PK252_12845"
     CDS             6549..10685
                     /locus_tag="PK252_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15639.1"
                     /translation="MSKVAKITLFFFFFAQVIRADESMKIDLYSYKDGLTTSSVNSTF
                     KDSKGYLWVCTANGLFRFDGYNFRNLNTLTSNPVSCEIISITEDAKHNLWLGSAGNGV
                     FFLEDQSNKLIHLNIRFEPNVRIYDMIYLNGRVWMATTKGLLIVDPEDVNDSDEPVKY
                     EIFIPDFKNKSPQARVINCIYHVEGSNTLWIGSNASLYAMNLNTKQFKGIYSHPQNSI
                     RSLSCFNGNIVAGSWDGGIFIVDTATYAKTSSPFVDYINAVIGDKRVVSVFYDVKGLM
                     YVATYSNGLYVFSGNKAGDYSYTNYRNDKNIYENIKTNTINHVLKDNDGVVWLSMNLP
                     ALAKIYKQESDISFFSPKRNNNEIKEFSTVSQSKLKDKLWITTNGDGMYLFNTKTKVF
                     EHFNSSANKGLKLHYDNVSRCFQDKKGNLWIVYSMNGLYVVPALYAAQLTEEKNTKVI
                     VPVNANEMISNDSKLNSYITNFYEDSKGRLWIGSWGALYLVSTNTDFQFARTTQDLIK
                     NSNTKCVYFDEYKEMVDFPIYPLISMVEIRNNNFWLGTFGGGIIELIENSPNNFTGHV
                     SELNKDLPQMNIKCLIKDKYNGIWIGTNNGLGYIGPGKSNLKVYSKNDGLLSDDINSI
                     VEDDKSNIWLSTNYGCILFNKPKNSIKNFLSLSNDDINQYIVKSGAVDNSGHVWFATS
                     EAVVTFDPDGEKNNMSKSSFRFTELKIDNKYIVPLEKVNGTRVIDKNLDYCTEVNIPY
                     NHTLSLTFAMLDYKAAGYTLFKYRISKKSEWIILNNGQRSINLSSLKPGKYLLEVEPV
                     NSNAGVQGISLQLNFLPPFWQTKVAFLIYFFFLLGMFWVYRRLTLQKLNEKKLVEIER
                     YERKKLEEMDKMKTEFFSNISHEFRTPLSLIINPLERFVKDAKYSDEEKERFRLILKS
                     SNRLLKLTNELMDFSKIEKNLLVPEFELCKMLDFINEICALFRNVADSMNIDFKVHCN
                     AGDIEIPVDKKMIEKVVFNLLSNAFKYTPNNGTVLVVIDKIENLDKQYAKISVINTGE
                     GISKENLSKIFDRFYQVNNVQNRNMEGTGIGLSLVKSYIELHNGIVEVKSIPNVETSF
                     EVFLPFVQPGFSDSAKKNSFEPMKVSIENPAMLKPTYHYHLLVIEDDEEIRNFIVDEL
                     KADFNISIAVNGEEGLRLANEQLPDLIITDVMMPRLSGIELCKKLKNQMSTSHIPIII
                     LSAKASTSNQIEGLEMGADVYMVKPFSIEHLKIQVLRLINFKEAIYSRYLKETSLLPD
                     GALASKLDEEFMKKIMAYIEENLTDTNLSVDQLAESVSLSRVQTYRKIKAITGLSAVE
                     FIRTIRLKKAAQLVVEKRLSYTEIAFETGFASASYFSKCFHDHFGKTPSEFATDYGEK
                     "
     gene            10998..>11312
                     /locus_tag="PK252_12850"
     CDS             10998..>11312
                     /locus_tag="PK252_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT15640.1"
                     /translation="MEKRTFTKEEKLRIIKKASENGVNPTLEKYGVYRASYYTWKQKF
                     EEMGEEGFHHGMPFVHRSLPIKAVCSILLRRIVTGLRPFNMACQGLAWFGLSRPKSFA
                     FSE"
BASE COUNT         3376 a         2051 c         2518 g         3367 t
ORIGIN      
        1 ttttcgtcag tattcatttt tttagaacaa agaacaaaga acaaagaaca aagaactatt
       61 gctatgagaa caaacactaa aaaattaaca ttggtattgg ctgtaatgac gttgtcattg
      121 aggcttttcg gttgggaagg tatgccaatg cctgctcttc acgttgaagg gcgatattta
      181 aaagatgctt cggggcatat tgtaaattta catggttttg cccagactta ttctccctgg
      241 ttcaatgagc agggaaaata ctggaccaat tatgatgtag ccggttgtct ggactacaat
      301 aaagggatta ttgatggtat tttggatgca ggatggaaag taaattttat ccgtttacac
      361 atggaccctt attggtctaa tactccggga gttgctgtta ctggtgaggc tgatatttcg
      421 gctttcgatt ttacccggtt taaaaaatac cttgatgaag tgtttatccc tatggccgaa
      481 tatgctattt ctaaagggtt gtatgttgtt atgcgccctc cgggtgtttg ccccgaaaaa
      541 attgcggtaa acgatacgta taataaatat cttatcaaag tgtggggcta tgtagcccgg
      601 catgctaaat tgaaaaacaa tccatacatc atgtttgagc tggccaatga gccggttaat
      661 attcttgggc ccgatggtac ttatggttcg ggttcgcaag gccattttga caacctgaaa
      721 acatactttc aggctgttgt ggacactatc agggccagtg ccaataatat tatttgggcc
      781 ccgggtttag gttatcaggc tttatatcag ggatatgccg taaatcctat tgaaggcgat
      841 aatattggtt atgctgtgca tgtttacccg ggttggttta atagcggaca gggatatgca
      901 aattttcaga aaggatggga tacccaggtt cagcctgttg ccgactttgc cccaattttg
      961 gttaccgaaa tggactgggc ccctgcaagg cacaataaat catggggaaa ggacagtact
     1021 ggtgttgccg ggaaaaatgg ttttggggct aattttaaaa aaattgtaga tgattgtggc
     1081 aatgttggtt ggttgctctt taccagccct gagcttttgg cccagtacgg agatgtcact
     1141 gcccctgccg atattaaaga ttttctgacc gaccctgaag catgccctaa gcctgtttat
     1201 caatggtatc aggcttatgc aagcgattac gggcttagtg gggcttctga aagttatttg
     1261 actgttacag gttttgttgt tgaaagtggc aaaaatatga ccatactgac taagggctcg
     1321 gccgggttaa aaattaacgc tgtttttgcc gacgggcata tcgaaaatgt aagctcattg
     1381 gctaaatttt cggttgataa aactgaggtg gtaaatgttg ccaggggcag ggtctttgct
     1441 ttggccgatg gacaagctac ggtaggaatt ctttataccg accctaaagg aaatgcctgg
     1501 cgtgatactt tgcatgtttc ctctaccact ttccccctga ccaaccagtt gtttaaccct
     1561 gatatttggg gcgatggcac ttttgacgag gctacaaaaa ccctgaccac cgggcaatgg
     1621 ggctttggag gttggaccta cagcaatggc ttagatattt cagggtacaa atatttagtc
     1681 gcaaaattag ggtcaaaaaa caatgccggc gtttcgttca gggtatttga cgaaaacaac
     1741 tactgggccg gcgctgccga gtatgatttt ggaaataagc agatagtagt ggccaatacg
     1801 gggtggatgc ctaagaaaga caaatcggct acgcttaatc cttcgcatat ttatattgtg
     1861 gggttttggt caaacggcaa tattcctttt gttattgaca ctgtattttt gtcaaactcc
     1921 agcgaatatg atgcattggc ttctgtaaaa aatgaaacaa ccaataaaaa caatgagcct
     1981 gaattagttg atgtatataa tttacttggt gtcaaaatta tgtcgcaggt gaaaaaagaa
     2041 caagccataa gagaattaaa taagggcgtt tatctggttg gcaaagaaaa ggttgtaata
     2101 acgagaaacc gtaaataata agcttattta taaattttta attcgaaaaa aatgaagcta
     2161 tttcgcaatt atttggctat gttgatgctt tttgcaattg gtatagccgg agcagcgcaa
     2221 actttccaaa aaaccgacgt tggcgtaaaa gccaaagctg gatcggtgaa tgttgaagtt
     2281 cagttctaca cttccgacat tgtaagggtt gtaaaatatc ccgagggtaa agcttttgaa
     2341 aaacaaagcc tttcggttgt tcttgcccct caaaaaatat cttttggcgt taagcaacaa
     2401 ggcgatgagt tggtgctgac aagccggaag atgcaggtat ctttaaacct gaaatcgggg
     2461 aaggttatat tttctgataa atcaggaacg ctcttaaatg aaaaggatgc cggggctgca
     2521 tttgctgatt ttaatgatgc cgggaacaag acttatactg tttcgcaggc ttttgccctt
     2581 gataaagacg aggctatata tggactcggg caacaacagc gtggtaaaat gtcgcaacga
     2641 aacgtgaagc tgcacatggt ccagaacaat accgaggatt ttatcccatt tttcgtatca
     2701 tccaaagggt atggggtatt ttgggacaat tattccccaa cggtttttga agataacact
     2761 gaaggtacat cgttcaaatc ggatgtgggc gatggaatag attattattt catggctggc
     2821 aacaatgccg atggcgtgat tgcttgtatg cgccagctta ccggacaagt ccccatgttc
     2881 cccttatgga catttggttt ctggcaaagc cgcgaacgct acaaatcgca ggatgagttg
     2941 acagaggtgg tcagaaaatt ccgcgaatta aatgttccaa tagatggtat catccaggat
     3001 tggcagtact ggggcaacaa ctacctgtgg aatgctatgg aatttctcaa ccctggcttt
     3061 tatgacccgc aaaaaatggt caatgacgtg cacaacatga atgcccatat aatcatttct
     3121 atctggtctt ctttcggtcc aatgacaaaa caatataaag agcttgataa aataaaagca
     3181 ttgtataata ttgaaacctg gccacaatcg ggttctgaga aatggccacc caatatggat
     3241 tatccatcag gggtgcgcgt gtatgacgct tacaatcccg aagcccgtga tatatattgg
     3301 gaccatttga acaaaggtat tttctcgctg ggcatggacg gatggtggat ggattctacc
     3361 gaacctgacc attttagttg gaaatctgaa gatatggaca caaaaacata tcttggctca
     3421 tttcgtaaag tgcgcaatgc ctatccttta atgactgttg gcggtgtata tcagcaccag
     3481 cgtgctgttt cgtccgacaa acgtgtgttt atccttacac gctcggcttt tgccggccaa
     3541 cagcgctatg gtgccaatac ctggtctggc gatgtggtgg cttcatggga tgctttgcgc
     3601 aaccaaattt cggccggctt aaacttttcg ctttgcggta tcccttattg gaacagcgat
     3661 ttgggaggat tcttcctgtg gaactttaaa aatcctcttg aaaatgccga ttatcgtgag
     3721 cttcatgttc gctggatcca atttggcgca ttctgcccta tgatgcgctc gcatggcgaa
     3781 ggctcgcccc gcgaattgta ccagtttggt aaaaaaggaa atgcaaccta cgatgctatt
     3841 gaaaaataca tcaaactcag gtatagccta ttgccttata tttattcttc gtcgtgggag
     3901 gtgaccaaca agcaatctac catgatgcgg gctttggtta tggattttgc ggccgacaga
     3961 caggcgctgg atatcaatga tgagtttttg ttcggaaaat cgctcctggt gagccctgtt
     4021 acgcagcctt tgtacctgaa aaacgaggtt cgcggaaaag ataccgttaa agtaacagat
     4081 ttttctactg ttaaaagtaa agctgtttat ctgcctaaag gagctgactg ggttgacttc
     4141 tggactggcg acaaattctc aggtggccaa accgtaaata aagaagtgcc tttggatatt
     4201 attccggtgt ttgtcaaggc gggctctatc cttcctattg gccctgaagt ccaatattcg
     4261 ggcgagaaga aatgggacaa cctcgaaata cgggtgtatg aaggggctaa tggcgaattt
     4321 accttgtacg aagatgaagg cgacaattat aactacgaaa aaggggctta ttcaaccgtt
     4381 tcgttttcgt ggaacaacca gaaacgcacg ctgtctattg gtgaaagaaa aggttcattc
     4441 ccgggaatgt tgacaaacag ggtttttaac attgtccttg ttggaaaagg taaaggtaca
     4501 ggctctgttc tttctgcaaa gtttgataag gcgataacct attcggggaa aaaacaggtg
     4561 gtgaagtttt gacataaaat taaacctgtt taaaactcat ggtcaaaagg ggcagctgat
     4621 ttccatctgt ttgagacagg ctattaaacc ttaaaaaata aaataaaaga tgaaacgagc
     4681 atgattcgtt aaacacaaac aaggatgaaa attcatcatg cgagttttcc ttcggagaac
     4741 ccttcggggt tcatccgacc ttataaaaaa aattatttac ggatgaaaaa gaacataata
     4801 ttcttattgc tgattgctgt tggggttagc ttttatgcac aaaaagcacc tgaaaaggaa
     4861 agtactccat atactaatcc tgtgatgtgg gcagatgtgc cagatatgtc cattgtacgc
     4921 acaggtagtg atttttacct gataagcact accatgcacc tgatgccggg cgccccggta
     4981 atgaggtcga aagaccttgt aaattgggag attgtcagtt atgtctttga cagtattact
     5041 gataattcaa aatacaactt attgaatggc actgtgtatg ggcgtggcca gtgggcttcg
     5101 tccatccgct atcacaatgg caagtattat gtactctttt cgcctaatga ccagcctttt
     5161 aggtcgtatg tttacgaaac ggcagaccct gccaagggaa actggaagct attggcccgg
     5221 atgcagcatt ttcatgacaa ttcactgttt tttgacgatg acaaccgcgt gtatgtgttt
     5281 tcggggacag gttcgctgcg cgagttgaaa agcgatttgt ctgatgtctt gcctggaggc
     5341 gttgatatgc agatttttca aagagattcg accgaaaacg ggctgttgga aggcagccag
     5401 gctttcaaat acaatggaaa atattacctg ctgatgatat cctggcccag gggcaagcca
     5461 cgcaggcagg tttgttaccg tgccgacaat attaccgggc cttacgagaa gaaagtgatt
     5521 ttgcaggaca attttgccgg tttcccatac tgtggacaag gctgtattac tgatgacgga
     5581 aagggaaact ggtttgccct tatttttcaa gacagaggtg gggtaggacg agtgccttta
     5641 ttgatgccgg tcagatgggt cgatggttgg ccaatgctgg gcgacgagaa cggaaaagtt
     5701 cctttgacag gttttacacc tctaaaaaca tataactcag gaaacaggat tgttgaaagt
     5761 gatgattttt cgggtaaaaa actgaagctt cagtggcaat ggaaccataa tcccgtgaac
     5821 agcgcatggt cgttgacaga acgtaaagga tatatgcggt taaagacaag ccgtgtggtt
     5881 gaaaacattt atttagcccc aaatactatt acacagcgta tggaaggccc taaatgcagt
     5941 ggcgttgtgt cgtttgatat ttcaaaaatg aaagatggcg acgttgccgg ttttagtgca
     6001 tttaatggaa atgctgccct tttgtctatt gttgtgctgg ggaacaagaa attcctgacc
     6061 atgtccacca atgttgtaga gcttgacaat gactcaaaag ccgtgcttaa agttgatgtg
     6121 gaggaaaagg ccagggttga gctgagcaaa gatgttattt ttctgcgtat cgatgccgat
     6181 tttaacctga ataaagattt ggctacgttt tattacagtt ttgacaacaa aaactggact
     6241 ccaataggcg ccgagtttaa aatgattttt gattaccgaa aattttttat ggggacaaag
     6301 tttgccctgt tcaattatgc tacaaagacc atcggaggct atgttgatgt tgactttttt
     6361 aattataaca ggattcaata aggctaagct ttcttttggg cgtttttcaa acgctaaatg
     6421 acctttaaaa accaaaatca aatgcatatt aaatttaaac gatattttaa gctggatttt
     6481 tttgaatatt tgatagtttt aatcatttgt ttgtctattt tggtgtttat ttggataatg
     6541 ctataaagat gagtaaagtt gcgaaaataa ccctgttttt tttcttcttt gcccaggtaa
     6601 tccgggcaga tgagtcaatg aaaattgacc tgtattcata caaagatggg ttgaccacgt
     6661 cttctgtcaa cagcacgttt aaagattcta aaggttattt gtgggtttgc actgcaaatg
     6721 gcctttttcg gtttgacggt tataattttc gaaaccttaa tacgcttacc agtaatcctg
     6781 tttcgtgcga aataataagc attacagaag acgctaaaca taacctttgg ttgggctcag
     6841 ctggcaatgg ggtgtttttt ttagaagacc aatctaataa attgattcat ctaaacatca
     6901 ggtttgaacc caatgtccgc atttatgata tgatttattt gaatggcagg gtttggatgg
     6961 ctaccacaaa aggcttgcta atagtggatc ccgaagatgt caatgattcg gatgagcctg
     7021 ttaaatatga gatttttatc cctgacttta aaaataaaag ccctcaagcc cgtgttatca
     7081 actgtatata tcatgttgag ggtagtaaca ccctttggat aggctccaat gcctcgcttt
     7141 atgcaatgaa tctcaataca aaacagttca aaggtatcta ttctcatcct caaaattcaa
     7201 tccgctcgct ttcttgtttc aacggaaata ttgttgccgg aagctgggat ggaggcatat
     7261 ttattgttga tacagccact tatgccaaaa cttcatctcc ttttgtagat tatatcaacg
     7321 ctgttattgg cgacaaaagg gtggtatctg tattttatga tgttaaaggg ttgatgtatg
     7381 tggcaactta tagtaacgga ttgtatgttt tttcgggcaa taaagccgga gattattcat
     7441 acaccaatta caggaatgac aagaatattt atgaaaatat caaaaccaat acaattaacc
     7501 atgttttgaa ggataacgat ggcgttgtat ggttgtcgat gaacctgcct gcgctggcca
     7561 aaatttacaa gcaggaaagt gatatttctt ttttttcgcc caaaagaaat aataatgaaa
     7621 taaaagaatt ttctacggtt tcgcaatcta aacttaagga caagctttgg ataaccacca
     7681 atggggacgg gatgtacctt ttcaatacaa agactaaggt ttttgagcat ttcaattcgt
     7741 cagcaaataa agggcttaag cttcattatg acaatgtgtc ccgctgtttt caagataaaa
     7801 agggcaacct ttggattgtt tattcaatga atggcttgta cgtggtgcct gctttgtatg
     7861 cagcccagct gactgaggaa aaaaatacaa aagtcatagt gcctgtcaat gcaaacgaaa
     7921 tgatttctaa tgacagtaag ctaaattctt atattacaaa cttctacgaa gacagcaaag
     7981 gccgtctgtg gataggtagc tggggcgctt tgtatttggt ctcgaccaat actgattttc
     8041 agtttgcccg taccactcag gaccttatca aaaacagtaa cacaaagtgc gtttattttg
     8101 atgagtataa agaaatggtt gattttccta tctatccgct catttcaatg gtagaaatac
     8161 gcaataacaa cttctggctg ggaactttcg gtgggggaat aattgaactt atcgaaaact
     8221 ccccaaataa ctttaccggc catgtttctg agctgaataa agatttgccc cagatgaaca
     8281 ttaaatgctt aattaaggac aagtataatg gcatttggat tggcacaaac aacgggcttg
     8341 ggtatatcgg ccccggaaaa agcaatttga aggtgtattc aaaaaatgat ggccttttgt
     8401 ctgacgatat caacagtatt gttgaagatg acaaatcaaa tatttggcta tcaacaaatt
     8461 atggctgcat tttgtttaac aaacctaaaa attctattaa aaactttctt tcattatcca
     8521 atgatgatat caatcagtat attgtaaaat ctggtgctgt tgataatagc ggccatgttt
     8581 ggtttgcaac aagcgaagcc gttgttactt ttgaccctga tggggagaaa aacaatatgt
     8641 cgaaaagcag cttccgtttt actgaattga aaatagataa taagtatatt gttcctcttg
     8701 aaaaggtaaa cggaacccgt gtcattgata aaaacctcga ttattgtaca gaagtcaaca
     8761 taccatacaa ccacaccctg agcttgacat ttgctatgct tgactataaa gctgccgggt
     8821 acacattgtt taaatacagg ataagcaaaa aaagtgaatg gattatcctc aacaatggac
     8881 agcgaagtat caacctttca agcctaaagc ctggcaaata cctgcttgaa gtagaacctg
     8941 tcaattcaaa tgctggtgta caaggtatta gcctgcaatt gaattttttg ccgccattct
     9001 ggcaaaccaa ggtggcattt ctgatttatt ttttcttttt gcttggtatg ttctgggttt
     9061 accgaaggtt gaccctgcaa aagctgaacg aaaaaaaact ggttgaaatt gaacgttacg
     9121 aaagaaaaaa acttgaagaa atggacaaaa tgaaaaccga gtttttttca aatatctcgc
     9181 atgagttccg tactcctctt agtttgatta tcaatccatt agaaaggttt gttaaagacg
     9241 caaagtattc tgatgaagaa aaagaacgct ttcgtctgat attgaagagt agcaaccgct
     9301 tgctcaagct taccaatgag ctgatggatt ttagcaaaat agaaaaaaat ctgcttgtcc
     9361 cggaatttga actttgcaaa atgcttgatt ttataaacga aatatgcgcc ttgtttagaa
     9421 atgttgccga ttctatgaat attgacttta aggtgcattg caatgccggg gacattgaaa
     9481 tacctgttga taaaaaaatg attgaaaaag tggttttcaa cttgttgtca aacgctttta
     9541 aatatacccc aaacaatggg acagtccttg ttgttattga taaaattgaa aatttagaca
     9601 agcaatatgc caaaatttct gttattaata ctggtgaagg tatctccaaa gaaaatttgt
     9661 ccaaaatttt tgaccgtttc tatcaggtaa ataatgtgca aaaccgcaat atggaaggga
     9721 cgggtattgg attgtccttg gtcaaaagct atatcgaact gcacaacggc attgttgaag
     9781 taaaaagtat ccctaatgtt gaaacttcat ttgaagtttt cttacctttt gttcagcccg
     9841 gtttttcaga tagtgcaaaa aagaatagct ttgagccaat gaaagttagc atcgaaaatc
     9901 cagctatgct taaacctacc tatcattacc atctgttggt gattgaagat gatgaagaaa
     9961 tccgtaattt tattgttgac gagctcaaag ctgatttcaa catttcaata gctgtcaatg
    10021 gcgaagaagg ccttaggttg gcaaatgaac aactccccga tttaattatt accgatgtga
    10081 tgatgcccag attgtcgggt attgagcttt gcaagaagct gaaaaaccaa atgagtacat
    10141 cgcatatccc tataattatt ttatctgcca aagcttctac cagcaaccag attgaaggcc
    10201 tcgaaatggg cgctgatgtt tacatggtca agccttttag catcgagcac cttaaaattc
    10261 aagtgcttag gttgatcaat tttaaagagg ccatttattc taggtatttg aaagaaactt
    10321 cattgctccc tgatggggct ttggcttcaa agctcgatga agagtttatg aaaaaaatca
    10381 tggcttatat tgaagaaaac cttaccgata ccaacctgag cgttgaccag cttgcagaaa
    10441 gcgtctcgct gagcagggta caaacatata gaaaaatcaa agctattacc ggtctttcgg
    10501 cagttgagtt tatccgtact atcaggttga aaaaagccgc ccagcttgtt gtcgaaaagc
    10561 gtcttagcta taccgaaatt gcctttgaaa caggttttgc ctctgcttct tatttttcaa
    10621 aatgttttca tgaccatttt ggaaaaaccc catctgagtt tgctacagat tatggagaaa
    10681 aataggtgac tggcgctttt caattcgtga ttttgaggtt aaacggattg tttgtttttg
    10741 agatattttc aacataaggt caataagcaa aaacatttgt aaaggccttt ttaacttggg
    10801 gcatatattt ttgtattcac aatttgaatc cgagtttcta tacttgtttc ctttttagag
    10861 aaaaaggtta caatgggacg gggaaatacc tgaaaaggcg tttccccgtt ttttttagta
    10921 gcacaacctt tttgtcatgt tttagccccc taaataatcg gacgaaagta tagataaaaa
    10981 tctgtacttt tatcattatg gaaaaaagaa cttttacaaa agaagaaaag cttcggataa
    11041 tcaaaaaagc ttccgaaaac ggagtgaacc cgacacttga aaaatacggg gtttatcgtg
    11101 catcgtatta cacgtggaag cagaaatttg aagaaatggg agaggaaggc tttcatcatg
    11161 gtatgccctt cgttcatcgt agcctgccaa taaaagcggt ctgtagcatc ctgctacgtc
    11221 gcatagtaac cggcctacgg ccgtttaaca tggcatgtca aggcttggcg tggtttggtt
    11281 tgagcaggcc taaaagcttt gcgttttctg aa
//
LOCUS       DAOSPF010000086        11037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5907_length_11037_cov_7.358131, whole genome shotgun sequence.
ACCESSION   DAOSPF010000086 DAOSPF010000000
VERSION     DAOSPF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5907_length_11037_cov_7.358131"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK252_12855"
     CDS             complement(<1..362)
                     /locus_tag="PK252_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15641.1"
                     /translation="MAAKETGVHILVIEDEPKVAQFIKNGLDESGFIADIAADGLQGK
                     TKALSGKYQLIVLDINLPHINGFQLCKIIRAERKDVPILMLTALSSLDDKIKGFDEGA
                     DDYLVKPFEFAELLARIKA"
     gene            539..2521
                     /locus_tag="PK252_12860"
     CDS             539..2521
                     /locus_tag="PK252_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT15642.1"
                     /translation="MQNTFSPVPLVRWLLPIILGITVRYYSSMDSFSAVWMSIISALL
                     LLLLLLIIKKSSKTWSFPFYLLLFVIGFAVADIQLTNVENTISHPEKSSFVAVIDEIP
                     AVKDSKTVAVTKVIAVKKDGQWIALDKKAIVSFIAVRDSVLRPGDVLLIKSGLSLPDP
                     PVFPMQFDYGLYCRNNGITYLAKVRKDDYLKLNITEKSLKYRLLNFRGFLLKRISHYF
                     GKNDEQSLFASLTLGYTASLTPELKSSYAKAGVMHVLSVSGLHVGIVYLYLSYLFWFL
                     KKSRKTLIIHSILVFVGLWLYAFISGMSSPVIRSTIMFSMFAFAGIIGRKGQSINILS
                     ASAIIILIVSPLQLFNVGFQLSYAAMAGIFIFYEPINSLMPGQNKIIKNISALVAVSL
                     AAQLSTLPFTLYYFHSFPLYFLVANLIIVPAASILLYASSSFMLFASFDNALTSFFAD
                     ILKVLYSFFNHLVRFIADLPWSTAYGFSFSFVDAVILAIIIAFVYLLLHKRESRLVIP
                     LLTIVLFLFVRYNTRKITSNTGTMVFLTEVSYNSQVVTVKQGDSLFHFLANKKYSLDW
                     ATQNFKAFYGIKDEKHIMLSELKISEELTFNGLSVLRLGENDFLVKDEKMLLHIKVLK
                     TGTIQKAECWKIDRSNLCKQNLISGLQRTANDKKVY"
     gene            2644..4002
                     /gene="trkA"
                     /locus_tag="PK252_12865"
     CDS             2644..4002
                     /gene="trkA"
                     /locus_tag="PK252_12865"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOT15643.1"
                     /translation="MKIVIAGAGDVGVHLTRMLAGENHEITLIDSREQRLKEIAADTD
                     IKSFTGLASSVDTLMRAGAGLADLFISVTSNEQANVMSAIISKSLGARKCLARIDNLE
                     YLDEDKRALFYELGIDYMFYPELLAANEITNQIQESASTDSMSFANGKLQLYVMRMEE
                     HAPAIDQTIEEFHNLTRDPEYKVVGIVRNNKTFIPTVHERFQEDDLALIITTPKGIDI
                     IVRNFGKKTNDIRSVMILGGSRVGINTARQLSLKQTVKVIEKDRTKSYELANMLRGAL
                     VINGDGRNFELLKDEGIGHIDAFIAVTGDPEINLITCLLAKQLGVKKTIAAIENSDYI
                     KLAENMDIDNVINKKSVTASKIFSFTLNDEVSSVKCLTCSEAEVVEYMAKPDSKVTQV
                     PLKEIKLPEGAVIGGVVRGKEAIIADDDVHIKPFDKVIVLAMPDTLHKVGKFFNPQDR
                     FF"
     gene            complement(3999..4892)
                     /locus_tag="PK252_12870"
     CDS             complement(3999..4892)
                     /locus_tag="PK252_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT15644.1"
                     /translation="MKNHNKKLVYLSVIIAMICWSMSFIWYKQAYLNFGPFATVFLRL
                     LISASILMCVAWFTGRLRIKREHYKLFFVAALFEPFLYFIGESIGMLSVSPVVAAVIV
                     ATIPVFTPIVSSIMYNERLNKANRVGIVVSFVGVLFVIVRKGAALDASFPGVMLMFLA
                     VLSAIAYALALRKLTPYYDSLTIVAIQNTIGMVLFLPLFILFDSKTAIANLSVNTLIP
                     IFYLAIFASSIAFILFAYGVAKLGISKANAFTNLIPVITSLFSYFFLKETFSSLKIAG
                     IILVVSGLFLTQLKFGKKTIG"
     gene            5312..6172
                     /locus_tag="PK252_12875"
     CDS             5312..6172
                     /locus_tag="PK252_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOT15645.1"
                     /translation="MRQLKITKSITNRDSESLEKYLSEIAKEEMITVEEEVQLAQRIK
                     KGDQKALERLVKANLRFVVSVAKQYQHQGLSLPDLINEGNVGLIKAAQKFDETKGFKF
                     ISYAVWWIRQSILQAIAEQSRIVRLPLNKIGTLNKIHKAFNQLEQEYQRTPQADEIAK
                     VVDIPEEKIASTLSMSGRPVSVDAPLVEGEESSLLDLMENKDSPMADNDLLNESLKKE
                     IQRTLATLSEKERIVIEMFYGLGKKEMSLEEIGQEIGLSRERTRQIKENALRRLQKSS
                     RNKLLKAYLG"
     gene            6317..7117
                     /locus_tag="PK252_12880"
     CDS             6317..7117
                     /locus_tag="PK252_12880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT15646.1"
                     /translation="MFFRISPAVKNLLIINVIMFIGTWAVTKTYGIDLNSYLGLYFFR
                     SEHFHFFQYVSYMFMHGGFWHIFMNMWALFMFGVALEQVWGSKRFLIFYFVTGIGAAL
                     VHTFVNYIELVPMVNSAHAFMNTPSPELFGSFLKNYVPDAPRQLYDFINQWSVDPTNQ
                     VYINEATNWVNKISEQAINIPTVGASGAVYGILLGFGMLFPNTQIMLLIPPIPLKAKY
                     FVLIFGGIELFMAFQQPGSQIAHFAHVGGMIFGFIMIKMWNKDRQHFY"
     gene            7124..7999
                     /locus_tag="PK252_12885"
     CDS             7124..7999
                     /locus_tag="PK252_12885"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT15647.1"
                     /translation="MDIIEDLKRQYKYGGTLIKLIYINVAVFIAISIVSLITYLVRGV
                     GYDISDFFAFPSNMHLFLLKPWTIFTYMFIHAGIWHILFNLLWLYWFGQIFLQYLTQK
                     QLLFVYISGGIVGALLYALAYNISPAFDMERFGSSLVGASGAIMAIVIAISAIVPDYS
                     INLLLFGKVKLKYIALFTIILDILSIQSSNAGGHIAHLGGALWGYIYAVRYRSGKDFS
                     KVFITFFSKITGVFKRRPKMKVSYKKPSDDKEYLKTKAEMQKETDRILDKIAKGGYGS
                     LTNDEKDFLFRQSNR"
     gene            8164..9099
                     /locus_tag="PK252_12890"
     CDS             8164..9099
                     /locus_tag="PK252_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT15648.1"
                     /translation="MMKRIASLLLMVFFATMVFAQDANDVNLIMANAKKKASQQDFTG
                     AIKEYNRALRMKPGIAEAYCGRAEAKYYLGQYAEAMHDVNKAIEANANEAKFFTIRGM
                     INAALKKTDDAIADFNKAIQVNSAYAEAYVQKSQVLFQSSKEKEAFELLNSKIEAMPN
                     EAALYFGRGILYSLKQKNEKALEDLNKCSNLDAQYNVYNVTLAKGTVFMALQEFHDAE
                     AAFSEAISIAPNNALGYSSRGKLYYDQSKFDMAVEDYKKAIECNPDNAALYFNMGMAY
                     FKQNDKRNACERFHKACSMGNNNACKMIILECSGK"
     gene            complement(9109..10509)
                     /locus_tag="PK252_12895"
     CDS             complement(9109..10509)
                     /locus_tag="PK252_12895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT15649.1"
                     /translation="MKIQFLGAAREVTGSKHLLTTDNGRRILLDCGIYQGKGMDTEEL
                     NRKLNFDPKEIDNIILTHAHIDHSGLIPYIYKLGFRGSVVCTSATRDLCAYMLADAGH
                     IHEQDTRTYNKKMQRLGKKPVEPIYTKQDAIDCMELFIGVPYDRKFYIDDKTRVRFTN
                     TGHLLGSAVANIEILEAGHTKRLAYTGDIGRPENRIIKSPSPFISADYIICESTYGNR
                     RHDNSLKADEDLLSIVHDTCIIKGGKLIIPSFSVGRTQEIVYSLNNFYNEKKLPKVDI
                     FVDSPLAMNVTNVFRLHADCFNKKILHIMENDPDPFGFNSLHYVKSVEESKQLNDYSK
                     PCVIISASGMAEAGRVKHHLANNISNPKNTVLMVGYCAPSTLGARLLRGEKNISIHGY
                     PYEVKAQICRLESYSGHADYVEIASFLSCQDKKRLQKMFLVHGDYEAQTELKTYLESK
                     GFANIIIPEKGSSYNM"
     gene            complement(10632..>11037)
                     /locus_tag="PK252_12900"
     CDS             complement(10632..>11037)
                     /locus_tag="PK252_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15650.1"
                     /translation="TSPTHEYQKADTTYNVCYTIIDPITSDSSTYCEPVEVKKSGVKT
                     ATTNNVQYTVYPNPAKDNITAILYIKQATDAKVDICDLSGRTIMNLNSKLKLETGSNS
                     IKFNINNLQYGSYILRFTTSSGDIYKKLIIKY"
BASE COUNT         3306 a         1859 c         2256 g         3616 t
ORIGIN      
        1 gctttgatac gagctaaaag ttcagcaaat tcaaatggtt tgacaaggta atcgtctgcc
       61 ccttcatcaa agcccttgat tttgtcgtca aggctgctta aggctgtcag catgagtatg
      121 ggaacgtctt ttctttcggc cctgatgatt ttacatagct gaaatccgtt gatatgcggc
      181 aggtttatat ccaaaacaat gagctgatat ttgcccgaca aagcttttgt ttttccctgt
      241 aagccatcgg cagcaatatc agcaataaat ccagattcgt ccaatccgtt tttgataaat
      301 tgggcaactt ttggctcatc ttctattact aaaatatgta cgccggtttc tttggcagcc
      361 atggtaaaaa tcttttgatt gcaaagtaaa taaatataag gcaaatgttt gcgaaagttt
      421 gtttttaaaa ttggtttgtt aagccttgaa aaaactcttt atagttttta aaagaaatcg
      481 catgtgttaa gagaaatttt tataacttgg ttttgaaaaa cccaaacctt tcagttttat
      541 gcaaaatact tttagccctg tacctctggt caggtggttg ttgcctatta ttttagggat
      601 aacagttcgg tattattctt caatggacag tttttcagct gtatggatgt cgataatcag
      661 cgcgttgcta ttactgttgc tgcttcttat tattaagaaa agcagtaaga catggtcgtt
      721 ccccttttat ctgttattgt ttgtgattgg ttttgctgtt gccgatattc aacttacgaa
      781 tgttgaaaat actatcagtc atcccgaaaa gtcctctttt gttgctgtta ttgatgaaat
      841 tcctgctgtt aaggattcga aaactgttgc tgttacaaag gtaatagctg ttaaaaaaga
      901 tgggcaatgg atagctttgg ataaaaaagc aattgtttca ttcatagctg tcagggattc
      961 tgtgcttaga ccaggagatg tgctgcttat aaaatccggg ctttcattgc ctgatcctcc
     1021 ggtttttcct atgcaatttg attatggttt gtattgccga aataatggaa ttacctattt
     1081 ggccaaagtt cggaaagatg attatttaaa actcaatata actgaaaaaa gcttgaagta
     1141 caggcttctc aactttcgtg gttttttact aaagcgaata agccattatt ttggcaaaaa
     1201 cgacgagcaa agtttgtttg catccctaac attgggctat acagcttcat taacccctga
     1261 actaaaatct tcatacgcca aagctggtgt tatgcatgtg ctttctgtat caggcttgca
     1321 tgttggtatt gtatatcttt acttgtctta tttgttctgg tttttaaaga aaagtagaaa
     1381 aaccctcatt attcactcta tcctggtatt tgtcggattg tggttatatg cttttatttc
     1441 gggcatgtcg tcacctgtaa tacgctccac tattatgttt tcgatgtttg cctttgcagg
     1501 gattattggc agaaaaggcc agtctatcaa tattttatca gcttcggcaa ttattattct
     1561 cattgttagc cctcttcagc ttttcaatgt gggttttcag ctttcgtacg cagcaatggc
     1621 agggattttt attttttatg aaccaatcaa ttctttgatg ccggggcaaa acaagattat
     1681 aaaaaacata tctgctttag tggcagtgtc cttggctgcc caactctcca cattgccctt
     1741 tactttgtat tattttcatt ctttcccatt gtacttttta gtggcaaacc tcattattgt
     1801 tcctgccgca tcgatactgt tatacgcaag ctctagcttt atgctgtttg cttcgtttga
     1861 taacgcctta acctcttttt ttgctgatat tcttaaagtg ttgtatagct tctttaatca
     1921 tttggtgcgt tttatcgccg acttaccttg gtcaacagcc tatggtttct ctttttcttt
     1981 tgtcgatgca gtgattcttg ctataatcat tgcatttgta tatttgcttc ttcataaaag
     2041 agaaagcaga cttgttattc cgttgctgac tattgttctt tttctttttg tcaggtataa
     2101 tacgaggaaa attacttcaa atacaggaac aatggtcttt ttaactgaag tgagctataa
     2161 tagtcaggtg gttactgtta agcagggaga ttcgttattt cattttctgg caaataaaaa
     2221 atacagcttg gattgggcaa cacagaattt taaagctttt tatggaataa aagatgagaa
     2281 acatattatg ttgtctgagt taaaaataag tgaagagttg acgtttaatg gattgtcagt
     2341 gttaagatta ggtgaaaatg attttcttgt aaaagatgaa aaaatgctgc ttcatatcaa
     2401 agtgctaaaa acggggacaa ttcaaaaagc tgaatgctgg aaaatcgatc gaagtaattt
     2461 gtgcaaacag aacttgataa gtggacttca aagaactgca aatgataaaa aggtttacta
     2521 aaaatggcct atatttacaa aagtatttgt tgaagcaaaa tagctaataa tcaccgtgtt
     2581 atttgtcaat ctgctccaaa tgcattttta aaccggattt tggacatctt taatttttaa
     2641 aaaatgaaaa ttgtaatagc tggggcgggt gatgtagggg tgcaccttac tcgtatgttg
     2701 gctggtgaaa atcatgaaat tacacttatc gactcgaggg agcaacgcct gaaagaaatt
     2761 gctgctgata cagatattaa gtcatttacc ggcctggcaa gttctgttga taccttaatg
     2821 cgggcaggag ccggattggc cgacttgttt atttcggtaa ccagcaatga acaggcaaat
     2881 gtcatgtcag ctattatttc aaaaagtctt ggtgcccgta aatgcttagc ccgcattgac
     2941 aaccttgaat atcttgatga agataaacga gcactgtttt atgagctggg aatcgactat
     3001 atgttctatc ctgagttgct tgccgccaat gaaattacaa accagataca ggaatcagct
     3061 tctactgact ctatgagctt tgccaatggc aagcttcagc tttatgtgat gcgcatggaa
     3121 gaacatgctc ccgctattga ccagactatt gaagagtttc acaatcttac acgtgacccg
     3181 gaatataaag ttgttggcat tgtacgtaat aacaagactt ttatacctac tgttcatgaa
     3241 cggtttcagg aagatgacct ggctcttatc attactactc caaaagggat agatattatt
     3301 gtccgcaatt ttggtaaaaa aactaacgac attcgttctg tgatgatact tggcggtagc
     3361 cgtgttggga taaacacagc ccgtcagctt tcgctgaaac agacagtaaa ggtcattgaa
     3421 aaagacagga ccaaaagcta tgagcttgcc aatatgctta ggggtgcgtt ggtcatcaat
     3481 ggagatggcc gaaattttga gcttctgaaa gatgaaggta ttggacatat tgatgctttt
     3541 atagctgtaa ccggtgaccc cgaaataaac cttattacct gccttttggc aaaacagctt
     3601 ggtgtaaaga agactattgc tgctattgaa aatagcgact atattaagtt ggccgaaaat
     3661 atggatattg ataacgttat taataaaaaa agcgttacgg caagtaagat atttagcttt
     3721 acacttaacg atgaggtgtc atctgtgaaa tgtttgacct gctctgaagc cgaagtagtt
     3781 gaatatatgg ccaaacctga ttcaaaagta acccaggtgc cgcttaaaga aattaaactg
     3841 cctgaagggg ctgttattgg tggggttgta agaggtaaag aagcgataat tgctgatgat
     3901 gatgttcaca ttaagccctt tgataaagta attgtactgg ctatgcctga taccttacat
     3961 aaggtcggaa aatttttcaa cccgcaggat aggttctttt aacctatagt tttcttgcca
     4021 aattttagtt gggtcaaaaa taaaccactt actacaagta ttattccggc aatttttaag
     4081 gaagaaaatg tttctttcag aaaaaaatac gaaaacaaag acgttattac cgggatgaga
     4141 ttggtaaaag cattagcttt agatatccca agtttggcaa caccataagc aaaaaggata
     4201 aatgctattg acgaagcaaa aatagccagg taaaatatcg ggatcaaagt gtttaccgat
     4261 aaatttgcta tagctgtttt gctatcaaat aagatgaaaa gaggaaggaa taaaaccata
     4321 ccaatagtgt tttgtatagc cactatcgtc aacgaatcgt aatacggggt tagtttgcgc
     4381 aatgccaggg cataagcaat ggcagacaat actgcaagaa acattaacat cactcccgga
     4441 aaagaagcgt ctaaagccgc ccccttacgc actatcacaa aaagcacacc tacaaaagaa
     4501 acaacaatgc caacgcgatt agctttattc agccgttcgt tgtacattat gctcgaaaca
     4561 ataggggtga aaaccgggat tgtagcaaca atcactgctg cgacaacggg tgaaacactt
     4621 aacataccaa tgctttcgcc aatgaaatat aaaaaaggct caaataatgc ggcaacaaaa
     4681 aataatttgt agtgttcgcg ttttattcta agccgcccgg taaaccaagc tacacacatt
     4741 aaaattgaag cagagattag gagccttaaa aagacggtag caaaagggcc aaaattcaaa
     4801 taagcttgtt tgtaccagat aaaagacatg ctccagcaaa tcatggcaat gattacgctc
     4861 aggtaaacca gctttttatt gtgatttttc aatttttttt ggcaaaagta acatttcact
     4921 gccaaatcca atgaataaat tactgtcaac ttcaagttaa aaaataacca aacatatttt
     4981 tttgcgtaaa aaacatagtg aatatgaaag aagcgttgtt tttttaactt ttgttagtga
     5041 aactttttga aggtattttc gtttaaagta agttagaaag ctccaataag tgaacctata
     5101 ttatagttta tttaagataa aaaatgacta aaactaattt ttgtacactt tttgatagat
     5161 agtttataat cattttgtaa attgcgggtt tatttttgta ttttataaaa cattgatatt
     5221 taatagttta tattttaaat tgttgaataa tttcaattat tccaaacata gtaagcaata
     5281 agttgttttt attataataa gcagaagaag gatgagacaa ttaaaaatta caaaatctat
     5341 taccaatcgt gatagtgagt cgctggaaaa gtaccttagc gagatagcta aggaagagat
     5401 gattactgtg gaagaggaag tacagctggc ccaacgtatc aaaaaaggag atcaaaaagc
     5461 gctcgaaaga cttgtaaaag ccaacttgcg ttttgtggtt tctgtggcaa aacaatatca
     5521 gcatcagggt ttaagtttgc ctgaccttat caacgaaggt aatgttggtt tgatcaaagc
     5581 tgcccaaaag tttgatgaaa ccaaaggttt taaatttatc tcctatgctg tttggtggat
     5641 caggcaatct atacttcagg ccattgccga gcaatcccgt attgttcgtt taccgttgaa
     5701 caaaattggt acattaaaca aaatccataa agcatttaac caacttgaac aagagtatca
     5761 acgtactcct caggctgatg aaatagctaa agttgtagat attcctgaag aaaaaatagc
     5821 aagtaccctc tcaatgtctg gtcgtccggt gtctgtagat gcacctttgg ttgaaggcga
     5881 agaaagctca ttgctcgacc tgatggaaaa caaggactcc cctatggctg ataatgacct
     5941 gctcaacgaa tcattgaaga aagaaattca gcgtacattg gctactctct ctgaaaaaga
     6001 acgtattgtt attgaaatgt tctatggatt gggcaagaaa gaaatgtcgc ttgaagaaat
     6061 agggcaggag attggccttt cgcgtgaacg tacgcgacaa ataaaagaaa atgctttgcg
     6121 ccgcctacaa aaaagtagcc gtaacaagct gttgaaagct tacctcggat aaatttgttg
     6181 aagtatttat atttacgcca acctgacaca aatatgctga caggttggct tttttattct
     6241 ttggcaaatg ttttgaaaga caaaacattt gaagctgtat tttgttattt ttgttgccat
     6301 aattaatatg tagaatatgt ttttcagaat ttcacctgct gtaaagaacc ttcttattat
     6361 caatgtcatt atgtttattg ggacctgggc ggtgacaaaa acttatggta ttgacctgaa
     6421 ttcttacctt gggctttatt tcttccgttc cgagcatttt catttttttc aatatgtttc
     6481 atatatgttc atgcatggtg gcttttggca tatttttatg aatatgtggg ccttgtttat
     6541 gtttggggtt gcattggaac aggtatgggg aagcaaaagg tttttgattt tttattttgt
     6601 tacaggtatc ggtgctgctt tggttcatac ttttgtaaat tatatagagc ttgtgcctat
     6661 ggtaaacagt gcacatgctt ttatgaatac cccttcgcca gagttgtttg gctcatttct
     6721 aaaaaactat gtgccggatg ccccaaggca gttgtacgat tttatcaacc aatggtctgt
     6781 tgaccctaca aatcaggtat atatcaacga agctaccaat tgggtgaata aaatttcgga
     6841 acaggctatt aatattccta cagtaggggc ttcgggggca gtttatggca tcttgttagg
     6901 ttttggtatg ttgtttccca atacgcagat tatgttatta atccctccaa tacctctcaa
     6961 agcaaaatac tttgttttaa tttttggtgg aatagaattg tttatggctt ttcagcaacc
     7021 tggaagccaa attgcccatt ttgcccatgt cggcgggatg atatttggct ttatcatgat
     7081 taaaatgtgg aacaaagaca gacagcattt ttattaatat actatggata ttatagaaga
     7141 cttaaaaagg caatataagt atggaggtac gcttataaag cttatttata tcaatgttgc
     7201 agtatttatt gcgataagta tagtgagcct tataacttat cttgttcggg gtgttggtta
     7261 tgatatcagc gacttttttg cttttccttc caatatgcac cttttcctgc taaaaccttg
     7321 gacaatcttt acttatatgt ttatccatgc agggatttgg catatattgt ttaacctgct
     7381 gtggttgtat tggtttggcc agatttttct tcaatatctc acgcaaaaac agttgttatt
     7441 tgtctatata tctggtggga tagtgggggc attgctatac gcattggctt ataatatttc
     7501 acctgcattt gatatggaac ggtttggctc ttcattagtg ggggcttcag gggctataat
     7561 ggctattgtt attgctatct cggctattgt gccagattat tcaatcaatc tactgctgtt
     7621 tgggaaggta aaactaaagt acatcgctct ttttaccatt attcttgata ttttaagtat
     7681 tcaatcttcc aatgccggcg ggcatattgc ccacttggga ggcgctttat ggggatatat
     7741 ttatgctgtc aggtatcgtt cgggaaaaga tttttcgaaa gtttttatta ctttttttag
     7801 taaaataaca ggcgttttta aacgcaggcc caaaatgaaa gttagttata aaaagccttc
     7861 tgacgataag gagtacctta agactaaggc tgaaatgcaa aaagaaacag accggattct
     7921 agataagatt gcaaaaggcg gatatggaag tttgactaat gatgaaaaag atttcttatt
     7981 caggcagtca aacaggtaat ggatttttat ttttcgcctt gttttcatac attcgctgaa
     8041 aagtattatt tttcaaaaga atttctaagt tgaagattat gaaacaccca tgattctgca
     8101 aatcgctgat tagatggtat ttatttgtgg gtgttccatc attcaattaa aataaaaata
     8161 attatgatga aaagaattgc ttcattgctg ctaatggttt tctttgctac catggttttt
     8221 gcccaggatg ctaatgatgt aaacctgatt atggctaatg ctaaaaagaa agcttctcaa
     8281 caggatttta ccggtgcaat aaaagaatat aacagggcct tgcgcatgaa accaggcatt
     8341 gctgaggctt attgtggaag ggctgaagct aaatattacc tgggtcagta tgctgaagct
     8401 atgcatgatg tcaataaagc tatagaagct aatgctaatg aagcaaagtt ttttactatt
     8461 cgggggatga ttaatgcggc tttaaaaaag acagatgatg caattgctga tttcaataaa
     8521 gctatacagg taaatagtgc ttatgctgag gcttatgtgc agaaatcgca ggtgcttttt
     8581 caatcatcaa aagaaaaaga agcatttgaa cttttgaata gcaaaattga ggctatgcct
     8641 aatgaggctg ctttgtattt tggccggggc attctttata gccttaagca gaagaatgaa
     8701 aaggcattgg aagatttgaa caaatgttcc aatttagatg cccagtataa cgtttataat
     8761 gtaaccttag ctaaaggtac tgtttttatg gcattgcagg agtttcatga tgcagaagct
     8821 gctttttctg aggctattag tattgcccct aataatgctt taggttatag tagccgtggc
     8881 aagctttatt atgaccaaag taagtttgat atggctgttg aagattataa aaaagctatt
     8941 gaatgtaacc ctgataatgc agccttatat ttcaacatgg ggatggcata tttcaagcaa
     9001 aatgacaaac gcaatgcttg cgaacgtttt cataaagctt gttctatggg aaataacaat
     9061 gcctgcaaaa tgattatcct tgaatgtagc ggaaaataat actttgggtt acatgttata
     9121 tgaactgcct ttttcaggaa taattatatt cgcaaatcct ttgctttcca aataagtttt
     9181 caactctgtc tgagcttcgt agtcgccatg tacgaggaac attttctgca ggcgtttctt
     9241 gtcctgacag gataagaatg aagctatctc aacataatcg gcatgcccgc tataagattc
     9301 cagcctgcat atttgagctt ttacctcgta agggtacccg tgaatagaaa tgtttttttc
     9361 gcccctcagc aggcgggccc caagtgttga aggagcacag taacctacca ttagcacggt
     9421 gtttttgggg ttagaaatat tgttggccaa atggtgtttt actcggccag cttcggccat
     9481 cccggatgct gagataatta cgcaaggttt cgaataatca tttaactgtt tagattcttc
     9541 aacacttttt acataatgta aagaattgaa accaaacgga tcggggtcgt tttccatgat
     9601 atgcaaaatc tttttgttga aacaatcggc atgcaggcga aaaacgtttg taacgttcat
     9661 ggccaacgga ctatcgacaa agatgtctac tttaggcagc tttttctcat tataaaaatt
     9721 gttgagggaa tatacaattt cttgtgtacg tcctacgctg aacgaaggga tgataagctt
     9781 gccgcctttt ataatacaag tgtcatgcac tatgcttaac aaatcttcgt cggctttcag
     9841 agaattgtcg tggcggcggt tgccataagt tgattcgcag ataatatagt cggcagaaat
     9901 gaaaggggat ggggatttga taatcctgtt ttcaggacgg ccaatatctc cggtataagc
     9961 aagccgtttg gtatgccctg cttcgagtat ttcaatgtta gctacagcac tgccaagtaa
    10021 atgcccggta ttggtaaagc gtacacgggt tttgtcatcg atgtagaatt tacggtcgta
    10081 tggtacaccg atgaaaagct ccatacagtc aatagcgtct tgtttggtat aaattggctc
    10141 gacaggtttt ttgccgagtc gttgcatttt tttgttgtag gtacgggtat cctgttcatg
    10201 gatgtggcct gcatctgcca gcatgtatgc gcaaaggtcg cgtgtggcgc ttgtgcatac
    10261 tacagaaccc ctgaatccaa gtttatatat atacggaata agccccgaat gatcaatatg
    10321 agcatgagta aggatgatat tatctatttc tttggggtcg aaattcagtt tgcggttaag
    10381 ctcttctgta tccatccctt tcccttgata aataccacaa tcgagtagta ttcttcttcc
    10441 gttgtcagtt gtcagtaaat gtttgctccc tgttacttca cgtgctgcgc ctaaaaactg
    10501 gattttcata acatgtttat tttttttgca aggtaataat aaagtatgaa gtttaaaggt
    10561 tttgtaatgt taagaaaaca aaaagaggct gccaaattct gacagcctct tttatgtatt
    10621 gtgtcaaatt cttaatattt gataatcagt tttttgtaaa tatcaccgga agaggtggtg
    10681 aaacgcaaga tatacgaccc atattgcaag ttgttgatgt taaacttaat ggaattgctg
    10741 ccagtttcaa gtttcagttt gctgttgagg ttcataatgg tgcgccctga caggtcacaa
    10801 atatcaactt tggcatcagt agcctgtttg atgtacaata ttgctgtgat gttatctttg
    10861 gccgggttag gataaacggt gtattggaca ttgttggttg ttgctgtttt tacaccagat
    10921 ttcttaactt ctacaggttc acagtatgta gatgagtcgc tggtaatagg gtcaattata
    10981 gtataacaaa cattgtaggt ggtatctgct ttttggtatt cgtgcgtagg cgaagtg
//
LOCUS       DAOSPF010000087        10944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_5977_length_10944_cov_6.707595, whole genome shotgun sequence.
ACCESSION   DAOSPF010000087 DAOSPF010000000
VERSION     DAOSPF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_10944_cov_6.707595"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PK252_12905"
     CDS             <1..976
                     /locus_tag="PK252_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT15651.1"
                     /translation="FNSATDGIILLDNNLKIMDINRASYEKTGYQYEELVHNTIFTLL
                     SKEGINIIQRQISALKSGGIVLNFETEIFIKNDGVIPVEVIATNINISGQEAILLIAR
                     DISERKKLERELLNSVINTEEKDRLQFSQELHDGIGPLLSAAKMYADWLAEPNKTSDS
                     TTIVPEIQKLINESTLAVRDLSFKLSPHILQNYGIIEALKAYADKVNKSGKVKVSVES
                     TELGRFDEIIEATSYRIICECITNTLKHADAKNISIHTVLENDTLIINYTDDGKGFDV
                     VKVLENRKGIGLLNMQSRLKSLNGTLAINSTLGKGTVFIIKIPLSYIE"
     gene            1022..1657
                     /locus_tag="PK252_12910"
     CDS             1022..1657
                     /locus_tag="PK252_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15652.1"
                     /translation="MIKIIIADDHTMFRESISKMFTMRKTVEVLAEASNGVELLALLD
                     HFSPDMVLMDIAMPEMDGIEATKKALAKQPNLKILTLSSFGDEQYYFQMVEAGAKGFV
                     LKNAGLSELEMAIKEVAKGGSWFSSELLQKVIVNLSAKNKKEDETGLTNREMEILKLI
                     CENLTNEQIAEKIHLSCDTVKWHRNNILSKTGCNNAIGLLIYAIKHKLVKI"
     gene            2357..2788
                     /locus_tag="PK252_12915"
     CDS             2357..2788
                     /locus_tag="PK252_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT15653.1"
                     /translation="MKTLNIILVDDNESFREALKKVLVTKYNANIIAEASSGEEFQQI
                     KILRKADVILMDVMIPDIDGITLTKKALWHEPSLKFIAITLHYDKVYLETLLEAGFKG
                     CIFKNNIFSELEPALETVINNRYYFPQDISIDGRDYFSIKK"
     gene            2795..4849
                     /locus_tag="PK252_12920"
     CDS             2795..4849
                     /locus_tag="PK252_12920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT15654.1"
                     /translation="MDMNQLKLKFIDEAESLLTSLDNVLIELEKTDDLQNINEAFRVM
                     HTIKGASGMFGFDKIVEITHELESIYDLLRQKTLQLNPSIIDITFTAADHIRALLIDE
                     AFSNENNIKRHKLLKESIDVVKGIKTPESKKTEPKTTSTPNEIKVSNKPTTGNIDKKA
                     TWNILFYPNEELIKRCVNMAYTFHDLFALGEYKISSKPFNNSEGEFWSIFLVTDQPFE
                     EIENALMFVMDYCKISKVADFDIFNPTELEKRDEQLNNITQHIEAFDTTDMQNNAPNN
                     ANDSGKELIHLINKTNNQQPVQMTPKATTRINVEASKLDTLMYLVSELVTTKSELLIA
                     LKKHDEEKSIDAAEKIEKLSKLFSDNALSIRMVSLHEMLNKFKRLIRDLAKQLGKQIE
                     FEIIGEDTELDKNIIDAIGEPIMHLIRNCIDHGIEIPEKRIAKGKPETGTIKFEAQKT
                     GNYVYITISDDGNGINTEYVYNKAVDKGFIQPGTNLTEKEIMDLIFLPGFSTAESLSN
                     VSGRGIGMDIVLKRIHDIRGEIIVSSTKNIGTAFTLKLQQTVSIISTLLVTSGKTTYA
                     IPIEDIEACNIELDENTTNTQSKLIRYNNELLPFYILKGYTMHAEDSSCSQKLIIINK
                     QSKRYAIIADTIIGEYQAVIKPLGKAFSEVKHLSGASLLGDGSIALLLDTDNVWFKTS
                     NN"
     gene            4871..5308
                     /locus_tag="PK252_12925"
     CDS             4871..5308
                     /locus_tag="PK252_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT15655.1"
                     /translation="MKANTYLTFKLGQELFAVNVDNVLEVLERQLITPVPDAPKNLLG
                     IINFRGEILPVLNTHLKFNLTVEDNEELERLLIVFEIGQDENKYSVAATADAVKDVIE
                     VANEQIKPVPENGISYDSKYLTGITNYNNQFVMLLNTIKAFSF"
     gene            5352..7646
                     /locus_tag="PK252_12930"
     CDS             5352..7646
                     /locus_tag="PK252_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT15656.1"
                     /translation="MKKLMIKSKILLGFGIIQLLIIVIATLGIIKMYQMNNASQDMAT
                     VHLPSVYHAGSMNTDKEQIRNKEYRYLSNVNDEEKAKTEKLTKEIANSYKEHLNIYQA
                     KLVKNDEEKMLIDSAAIYFEQYMKLHEQIIAFSNEKKMDKARELMAGEARIVFYTWTE
                     VLDKLVELNKDEAATIAASSDKAFGILFYVVIIGVLFGSIMCIIMAFYIANGISRNLS
                     QINEAARNIAQGNLDINMQIDTKDEIYALAQSFEVMINALKSLINDTQMLAKASYDGE
                     LNKRANIEKHQGDYQKIVKGMNETLDGIVAPLNVTSEYLDKISQGIIPEKITKEYKGE
                     YNNLKNSINRCIDGLAGLIEANLVMQKLAVSDATLKVQGSYQGIFAQIADATNKVYDS
                     FNNISNAIELFAIGDFKTLNEMNQSFVKLSENDKIVPAFKNLNAAMNQIAEKAKQIAE
                     GDLTVTLEKRSENDILMQALNDMVTRLNEIVVQIMESAQNVSAGSSQLSSTSVQIAQG
                     ANEQASAAEEVSSSIEEMNSTIQQNTDNAVQTEKIAKSAYKGVEDVSNAAMKSLEATK
                     QIAEKIKIINSIAEKTDILAINAAIEAARAGEHGKGFAVVAAEVRKLAETSQKAAVEI
                     NALSASSLKLTLEGGELMSKLIPEIQRTSTLVQEIAAASNEQNSGAMQIAKAIEQLSS
                     VTQENSASAEEMSSTAEELASQAEALQETIGFFTTSKNASVDTKSTQQAKQITIDNKV
                     KPLKKKIIKTTDVKDQKDIDFESF"
     gene            7931..8086
                     /locus_tag="PK252_12935"
     CDS             7931..8086
                     /locus_tag="PK252_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT15657.1"
                     /translation="MAVGSPADVTKAVKDLLDSITDKPKVLLSCGGGMPPNVSTENIR
                     AFIEATK"
     gene            8163..9128
                     /locus_tag="PK252_12940"
     CDS             8163..9128
                     /locus_tag="PK252_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15658.1"
                     /translation="MKKLPFEIKITLIYIFLGAMWILFSDRLVVSFTSDIRKIQTLST
                     YKGWFYVLTTGLLFYFLKKKEIKRRNELYHELLEAKKKAEEADRLKSAFLANLSHYIR
                     TPMNSILGFVELLKEKDLSAQKQELFLSYINSQSYHLLQVISSIIEISKIQENQYEIN
                     KSLFSLNELLRKIVINANIEIKRLNKPVVVNAPFEMTGQNIELYTDKDIVEQILSNLA
                     SNAVNFTPKGEIMIGYNISEKQVEIFVKDTGVGVSEETQKALFKDFLYTSFYTCSEGE
                     GAGLGLHLSSKLAMLIGGELKLKYTNKNGSEFCLYLPLNLRADAN"
     gene            complement(9145..>10944)
                     /locus_tag="PK252_12945"
     CDS             complement(9145..>10944)
                     /locus_tag="PK252_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15659.1"
                     /translation="GGTNGYNCFHPDSIKENLVVPSVAIVDFKLFNKSVGVGELVRGK
                     AILKKNILETDKIVLSYMDNVISFEFAALQYNSPEFNQFTYILEGYEKEWNNVYNKTK
                     ATYVKLPPGEYVFKVKAANNDGVWNETGKALKIIILPPWWATWWFKVLIALLLSLVAG
                     YILFRVYVNITLKANQTILNERNQLKTLINNMPDYIFIKDTKSRFIVINKAKVQFMGA
                     KDEKEFLNKTDFDFYPKEIAEAYYKEEQEIMASGVALVNQESKRIVNGQERVLSTTKC
                     PIFSSEGKVIGLVGILKDVTEQKQAQEEILRQSQELQAYNIVLNETNVLLEERQQQIE
                     EQAEEIKITNEQLVEQQTRVLEQAEELKAQRDQLSLLNTTKDKLFSILAHDLRGPFNA
                     LIGYSELLIKNFKNYPPEKVETQLNFILDSSKQTFYMLTNLLDWSRSQREVISFHPEP
                     MAFSVCVSNELRVLSQLAQRKDLKITEVVNGSQKLINADPNLMGTVVRNLVSNAIKYS
                     FKGKTIYLTTNYADTEMIFSVRDEGEGLTPVVKENLFKVTNIDSKPGTIGEKGTGLGL
                     LICQDFIRKHNGKIWVESKMGEGSEFFISIPLA"
BASE COUNT         3755 a         2105 c         2011 g         3073 t
ORIGIN      
        1 atttaattcg gctacagatg gcataatact gcttgacaac aacttgaaaa taatggatat
       61 taaccgggct tcctacgaaa aaacaggcta ccaatatgaa gaattagtcc ataataccat
      121 attcacgctt ttgagtaaag aaggtatcaa tataatccaa cgccaaatta gcgcattaaa
      181 atcaggcggt attgtcctca attttgaaac cgagattttt ataaagaatg atggagtaat
      241 accagtagaa gtaattgcaa caaatatcaa cattagcgga caggaagcca tcttgctaat
      301 agcccgcgac atctccgaac gcaaaaaatt agagcgggaa ttacttaaca gcgttattaa
      361 taccgaagaa aaagaccgtc ttcagttttc gcaagagctg catgatggca ttgggcctct
      421 tttgtcggca gccaaaatgt acgcagactg gctggccgag cccaataaaa catcagattc
      481 aacaactatt gtccccgaaa tacagaaact cattaacgaa tcaactttag cagtaaggga
      541 cttgtcattc aagctaagcc cgcatattct gcagaattat ggtattattg aggcccttaa
      601 agcttatgcc gacaaagtaa ataagtcagg taaggttaag gtctctgttg aaagtactga
      661 gcttgggcgt tttgatgaaa ttatagaggc tacttcgtat cgtattattt gcgaatgcat
      721 taccaatact ttaaaacatg ctgatgcaaa aaatatcagc attcacactg tattagaaaa
      781 tgacacactc attattaact atactgatga tggcaaaggg tttgatgttg taaaagtatt
      841 ggaaaaccga aaaggaatag ggttactgaa tatgcaaagc cgcttaaaat cattgaacgg
      901 gacactcgct ataaatagca ccttaggtaa aggtacagta tttattataa aaatcccttt
      961 atcttatata gaatagactt tgaaatttta atacttttat aattgaccat cacacaaaac
     1021 aatgattaag attatcatag cagacgacca caccatgttt cgcgaaagca taagcaaaat
     1081 gttcaccatg cgaaaaactg tagaagtgct tgccgaagca tcaaacggag ttgaactttt
     1141 agcgcttctt gaccatttta gcccggatat ggtacttatg gacattgcca tgcccgagat
     1201 ggatggcatt gaagctacca aaaaagcctt ggccaaacag cctaacctga aaatactgac
     1261 actctcgtct tttggcgacg aacagtatta ctttcagatg gtcgaagccg gggctaaagg
     1321 ttttgttttg aagaatgccg ggctttctga acttgaaatg gccatcaaag aggtagccaa
     1381 aggcgggagc tggttttcgt ctgagctttt acaaaaagtc atagtcaatt tgagtgcaaa
     1441 aaataaaaaa gaagacgaaa ccggccttac caaccgcgaa atggaaattt tgaaactaat
     1501 atgcgaaaac ctcaccaacg aacaaatagc cgaaaaaatt caccttagct gcgatacggt
     1561 caaatggcac cgcaacaaca tcttgtccaa aacaggctgt aacaacgcaa tagggctgct
     1621 tatttatgct ataaagcata agcttgtgaa gatttgacaa ttaactggat ttctatataa
     1681 gaaaaatagc taatgtttta aactatagct tcttaacttt tgtacttgtt cagcttaggc
     1741 tttgcctgag ctgagtttat gccgtcaggc tataagcaga aaagtttcca tgaaacaggc
     1801 gaagcattaa aaagaaaaaa ttccttttca tcgccttttt gttcgatttc ggccgagaag
     1861 cctaacactc ctgttgtttg tttattgcta cattaccagg cttcgagggt gaaagagtta
     1921 aggcgataaa agtttttgag caactttttt cgacaaaaaa gttgcataga gacttagtat
     1981 ttattgctat tcaaatgttt taataataat agacaatgtt attcttatat cgaacacagg
     2041 ttgaatagca aatcaaatct gccgtggcat ggaaactttt ctgtttagtt aatgtctgta
     2101 aatatcagat acataagcat actacttctt tgcttaatga cttttaacct ataatgtaaa
     2161 aatcttgtca aaccaagcat acaactttat gtgtatttta tccccctaca ttgagatggg
     2221 gtacccccct ttttaacaat ggcccaaaac cattccctta ttggttaaat ccatttttct
     2281 ggccggtgtt tttgcttgtc gggcaaaata actttggact aacaattaca caagtaaaaa
     2341 atcaaattaa ggggatatga aaacgttaaa tataattctg gtcgatgata acgaatcctt
     2401 tagagaggca ttgaaaaaag tactggttac aaaatacaat gccaatatta ttgcagaagc
     2461 atcaagcggt gaagaatttc agcaaatcaa aattcttcga aaagctgatg taatccttat
     2521 ggatgttatg ataccagaca tagatggtat tacacttacc aaaaaagcac tgtggcacga
     2581 acccagcttg aaatttatag caatcacctt gcattatgac aaagtatatc tggaaacatt
     2641 actcgaagct ggatttaaag gctgtatttt caaaaacaac attttttcag aactcgaacc
     2701 agccttagaa accgtaataa acaatagata ctacttccct caggatattt ccatcgatgg
     2761 tagggattat ttttcaatca aaaaatgatt aaccatggat atgaatcaat taaaactgaa
     2821 gtttattgat gaagccgaat cacttttaac cagtttagac aacgtactta tcgagctgga
     2881 aaagaccgac gacctgcaaa atatcaacga ggcattcagg gtgatgcata ctattaaagg
     2941 ggccagcggc atgtttggct ttgacaaaat agttgaaata acgcacgagc tcgaaagtat
     3001 ttatgaccta ctgcgtcaaa agacgctgca attgaaccct tctataattg atattacctt
     3061 cactgctgct gaccacataa gggcgttact cattgacgaa gctttcagca acgaaaacaa
     3121 tatcaaaagg cataagttgc ttaaagaatc tatagacgtt gtcaaaggta taaaaacccc
     3181 agaaagtaaa aagacagaac ccaaaacaac ctcaactccc aatgaaatta aagtatcaaa
     3241 caaacccact actggcaata tagataaaaa ggctacatgg aacatattgt tttacccaaa
     3301 cgaagaattg ataaaacgtt gtgtaaacat ggcctataca tttcatgacc tgtttgctct
     3361 tggcgagtat aaaatttcaa gcaagccatt taacaatagc gaaggcgaat tttggagcat
     3421 tttcctcgtt actgaccaac cttttgaaga aattgagaat gccctgatgt ttgttatgga
     3481 ctattgcaaa atatcaaaag ttgcagattt tgacattttc aacccaacag aacttgaaaa
     3541 acgcgatgaa caattgaata atattactca acatatagaa gcctttgaca caactgatat
     3601 gcaaaacaat gcacctaata atgcaaatga ttccggcaaa gaactcattc atttgattaa
     3661 caaaaccaat aaccagcaac cagtacaaat gacacctaaa gctacaactc gcatcaatgt
     3721 tgaagcttca aaacttgaca cactgatgta cctcgtaagt gaattggtta caacaaaatc
     3781 cgagctgctt attgctctca aaaaacatga tgaagaaaaa tccatagatg ccgccgaaaa
     3841 aattgaaaaa ctttctaagc tttttagtga caacgccctg agcatccgca tggtttcttt
     3901 gcacgaaatg ttgaataaat tcaaaaggct catccgcgat ttggccaagc aactgggcaa
     3961 acaaatcgaa tttgaaatta ttggagaaga tactgagctt gataaaaaca tcattgatgc
     4021 tataggagag ccaataatgc accttatccg caactgcata gatcacggta tagaaatacc
     4081 cgaaaaacgc atagcaaaag ggaaaccaga aaccggaaca attaagttcg aggcccagaa
     4141 aacaggcaat tacgtatata tcaccattag tgacgatggt aatggcatta atactgaata
     4201 cgtttataac aaagctgtcg ataaaggctt tatacaaccc ggaacaaatc tgactgaaaa
     4261 agaaatcatg gacttaatat tccttccggg tttttcaaca gccgaaagcc tttcaaatgt
     4321 ctcaggcagg ggcataggaa tggatattgt cttaaaaaga atccatgaca ttcggggcga
     4381 gattatagtc tcttcaacca aaaacatagg aacagccttt accttaaaat tgcaacaaac
     4441 cgtatccatt attagcactt tgttggtaac atctggcaaa acaacatacg ctatacctat
     4501 tgaagatatt gaagcctgca atatcgagct tgacgaaaac accaccaata cccagagtaa
     4561 acttatcaga tataacaacg aactactccc attttacatt ttaaagggct atacaatgca
     4621 tgcagaagac agcagttgct ctcaaaaact gattatcatc aataaacaat ctaagcgata
     4681 cgccatcatt gcagatacaa ttattggaga ataccaggca gttattaagc cgttgggaaa
     4741 agctttttcc gaggtaaagc atttgtctgg tgccagtttg cttggagacg gaagtatagc
     4801 tttattgctc gataccgata atgtatggtt taaaacatct aataattagg aacttaaaaa
     4861 agtaagtacg atgaaagcca acacatattt gacattcaaa cttggacaag aactttttgc
     4921 cgtaaatgtt gacaatgtac ttgaggtgct cgaaagacag ctgattactc ctgtgcccga
     4981 tgctcccaag aatctattag gcattatcaa ctttaggggc gaaatcttac ctgttttaaa
     5041 cacacatctg aaatttaacc ttacggttga agataacgag gagttggagc gacttttaat
     5101 agtatttgaa ataggccagg acgaaaacaa atattcggtc gcagctacag ccgatgctgt
     5161 taaagatgta atcgaggtcg ctaacgaaca aattaaacca gttcccgaaa acgggataag
     5221 ctatgacagc aagtacctga ctggaataac aaactataac aaccaatttg tcatgctgct
     5281 caatacaatc aaagcattca gtttttaaaa taaaaaaata caatttaaat attcactaaa
     5341 aatcaatagt catgaaaaaa ttgatgatca aatcaaaaat ccttcttggg ttcggaatta
     5401 tccagttatt aataattgtg atagccacct taggaataat aaaaatgtac caaatgaaca
     5461 atgccagcca agatatggct acagtccatt taccttcagt ttatcatgcc gggagcatga
     5521 atacagacaa agaacaaatc cgtaataaag aatatcgtta cttatcaaac gtcaatgatg
     5581 aagaaaaagc taagacagaa aaacttacta aagaaattgc caattcttac aaagagcacc
     5641 ttaatattta tcaggcaaaa ttggtaaaaa atgatgaaga aaagatgttg attgactcag
     5701 cagctatata tttcgagcag tacatgaaat tgcacgagca gattattgct ttttcaaatg
     5761 agaaaaaaat ggataaagcc cgtgaattga tggctggtga agcccgaata gttttttata
     5821 cctggacaga agtccttgat aaattggtag agttgaataa agacgaggct gcaactatag
     5881 ctgcttcttc agataaagca tttggcatcc tgttttatgt agtaattatt ggagtgctct
     5941 ttggcagcat tatgtgtatc attatggcat tctacattgc caacggaata tcaagaaatc
     6001 tgagccagat aaatgaagca gcccggaata ttgctcaagg taacctggat atcaacatgc
     6061 aaattgatac caaagatgag atatacgctc tcgcccagtc attcgaagta atgataaatg
     6121 ccttaaaatc actaatcaat gacacccaga tgttagccaa agccagttat gatggcgaac
     6181 tgaacaaacg cgccaatatt gaaaaacatc agggcgatta tcaaaaaatt gttaaaggga
     6241 tgaatgaaac cttagatggc attgttgcac ctttgaatgt tacaagtgaa tatcttgata
     6301 aaatctctca aggcatcatt cccgaaaaaa ttaccaagga atacaaaggc gaatacaaca
     6361 atttgaaaaa cagcatcaac cgctgcatcg atggattagc agggcttatt gaagccaatt
     6421 tggtaatgca aaagctggca gttagtgatg caactttaaa ggtacaaggt tcataccagg
     6481 ggatatttgc tcaaatagct gatgccacca ataaagtata tgattctttc aataatatat
     6541 ctaatgccat tgaattattt gctattggtg atttcaaaac cctcaacgaa atgaaccagt
     6601 catttgtaaa actatcagaa aatgataaaa ttgtgccggc attcaagaat ttaaacgctg
     6661 ccatgaacca aattgccgaa aaagcaaaac aaatagcaga aggtgattta acagtaacgc
     6721 tcgaaaaacg gtcagaaaat gacatcctaa tgcaagcact aaacgatatg gtaacgcgtt
     6781 tgaatgaaat cgtagtacaa atcatggaat cagcacaaaa tgtatctgcg ggcagtagcc
     6841 aacttagcag cacttcagta caaatagcac agggagccaa cgagcaagct tctgcagccg
     6901 aagaagtatc ttcttctatt gaagaaatga actccaccat acagcaaaac actgacaatg
     6961 ctgttcaaac agaaaaaata gccaaatcgg catacaaagg cgttgaagat gtgagcaatg
     7021 ctgccatgaa gagcctggag gcaacaaaac aaatcgccga gaaaatcaaa atcatcaact
     7081 ccattgctga aaagactgat atacttgcca tcaatgctgc cattgaagca gcccgtgctg
     7141 gtgaacacgg aaaaggcttt gccgttgttg ctgccgaagt acgcaaactt gccgaaacca
     7201 gtcaaaaagc tgccgttgaa ataaacgctc tttcagcttc aagccttaag cttactctgg
     7261 aaggcggtga actaatgtct aaattgatac ccgaaataca gcgcacctca accttggttc
     7321 aagaaatagc tgctgcaagt aacgaacaaa actcaggcgc tatgcaaatt gccaaagcca
     7381 ttgagcaact ctcatctgta acccaggaaa attcagcctc agctgaagaa atgagctcaa
     7441 cagccgaaga attagcttct caggcagaag ctttgcaaga aacaattggt ttttttacca
     7501 caagtaaaaa cgcgtctgtt gacactaaaa gtacccaaca ggctaaacaa atcacaatag
     7561 acaataaagt caaacctcta aaaaagaaaa taatcaaaac tactgatgtt aaagaccaga
     7621 aagatatcga ttttgaatcg ttctaagtct cgtcaataaa aatagctatt gctgtcataa
     7681 tcctccggga gtataaagca cccggagggt ttttaaaatt ttctatctct acaaacaatg
     7741 ttctccttta caggttttac actgataagg cctatttaca aactctttac ctataacgca
     7801 caaccctact aaccgatttt accgggttca tcagggctga cccggtaagt tctataggta
     7861 ttgtcccttt cgggaaaaag cctcctaatg attaattggt gtttaacctt agcatccctg
     7921 taaatagcct ttggctgtcg gaagccctgc cgatgttaca aaagccgtta aagacctgct
     7981 tgacagtata acagacaagc caaaagtgct tctttcgtgc ggagggggca tgccgcctaa
     8041 tgtttctacc gaaaatatca gggcttttat tgaggctaca aaataattac tacttttttt
     8101 atttcgagaa gggtaaatgc ccgcgatttt tataatttta tgttacttaa ccaataagta
     8161 atatgaaaaa gctccctttt gaaataaaga ttaccctgat atatatattt ctgggggcaa
     8221 tgtggatact gttttctgac aggcttgtgg tcagttttac cagtgatata cgcaaaatac
     8281 aaacactatc gacatacaaa ggctggtttt acgtattaac caccggcttg ctgttttact
     8341 ttttaaaaaa aaaagaaata aaacgccgaa atgagcttta tcacgagctt ttggaagcca
     8401 aaaagaaagc cgaagaagct gaccgtctca aatctgcatt cctagccaac ctttcgcatt
     8461 atatccgcac accaatgaac agtatccttg gatttgtaga actgttgaaa gaaaaagacc
     8521 tttcagctca aaaacaggag ctttttttgt cttatattaa tagccaaagc tatcaccttc
     8581 ttcaggtaat ttcgagtatt attgaaattt ctaagattca ggaaaaccaa tacgaaatca
     8641 ataaaagctt atttagcctc aacgaacttt tgcgcaagat agtcatcaat gcaaatattg
     8701 agataaaaag gctgaacaaa cctgttgttg taaatgcccc ttttgaaatg acaggccaaa
     8761 atattgaact atataccgat aaggatatag tagagcagat attatcaaac ctggcatcaa
     8821 atgctgtcaa ttttaccccg aaaggagaaa tcatgattgg ttacaacata agcgaaaaac
     8881 aggttgaaat atttgttaaa gatacaggcg taggtgtttc ggaagagaca caaaaagcat
     8941 tgtttaaaga ttttctttac acttcttttt acacttgttc ggaaggcgaa ggagctggtt
     9001 taggcttgca tctttcatca aaattggcaa tgcttattgg tggagaattg aaattgaagt
     9061 acacaaacaa aaatggcagt gaattttgct tatacctgcc attgaatttg agagctgacg
     9121 ctaattagac aaaagttaga tatttcaagc cagcggtatt gaaataaaaa actcgctccc
     9181 ctctcccatt ttgctttcga cccatatttt gccattatgt ttacgaataa aatcctgaca
     9241 aatcaacaag cccaggcccg tgcctttttc gcctatggta ccaggttttg agtcaatatt
     9301 agttacctta aaaaggtttt cttttaccac aggagttaac ccttcgcctt catccctaac
     9361 cgaaaatatc atttcggtat ctgcataatt tgtagtaaga tagatggttt tgcctttaaa
     9421 actatattta atagcattag atacgaggtt acgaacaaca gtgcccatca ggttagggtc
     9481 tgcatttatt aatttttggg agccattaac cacctctgtt atttttaaat cttttctttg
     9541 agccagttgg cttaaaacgc gcaattcatt actaacacac actgaaaaag ccattggttc
     9601 aggatggaag ctgattacct ctctttgcga cctggaccaa tcaagcagat tggtaagcat
     9661 ataaaatgtt tgttttgacg aatcgaggat aaaattcaac tgggtctcca ctttttcagg
     9721 aggataattt ttgaaatttt taattaggag ctcgctataa cctatcaggg cattgaaagg
     9781 ccctcttaag tcatgagcca ggatagaaaa caatttatct ttggtggtat tgagaaggct
     9841 cagctggtcg cgctgggctt taagctcttc ggcctgttca agcacccttg tctgttgttc
     9901 cacaagctgt tcattggtta ttttaatctc ttcagcctgt tcttcaattt gttgctgacg
     9961 ttcttcaagc agtacattgg tttcgttgag tacaatatta tatgcctgaa gttcctggct
    10021 ttgccgcaaa atttcttcct gagcttgttt ttgttctgtt acatccttta aaatgccgac
    10081 taacccgatt acttttcctt cgctgctgaa aatagggcat ttggttgtag aaaggacccg
    10141 ttcttgcccg ttgacgattc ttttactttc ttgattgaca agagctaccc cggaagccat
    10201 aatctcttgt tcttctttat aataagcttc ggctatttct ttgggataaa aatcaaaatc
    10261 agttttgtta aggaactctt tctcatcttt agcccccata aactgtacct tagctttgtt
    10321 gatgacaata aagcgagatt tggtgtcttt aataaaaata tagtcgggca tgttgtttat
    10381 cagcgttttg agttggttac gctcgttcag tatagtttgg tttgctttca gggtaatatt
    10441 gacatatacc ctgaatagaa tatacccggc caccaatgac aacaacaatg ctataaggac
    10501 tttaaaccac catgtagccc accagggagg aagtataata atttttaggg ctttcccggt
    10561 ctcgttccac acgccatcgt tattggctgc tttcacttta aaaacatatt cgccgggagg
    10621 taatttgacg tatgttgctt tagtcttgtt atatacattg ttccattctt tttcatatcc
    10681 ttccagaatg taggtaaatt ggttaaattc tggagaatta tattgtaaag cagcaaattc
    10741 aaaagaaatt acattgtcca tataggaaag gacaatttta tccgtttcaa ggatgttttt
    10801 tttgagtatg gcttttcctc tgacaagctc gccaactcca acacttttat tgaatagttt
    10861 aaaatcaaca atcgccacag aggggacaac aaggttttct ttgatactat cgggatgaaa
    10921 acaattgtag ccgtttgttc ctcc
//
LOCUS       DAOSPF010000088        10880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6020_length_10880_cov_6.282771, whole genome shotgun sequence.
ACCESSION   DAOSPF010000088 DAOSPF010000000
VERSION     DAOSPF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6020_length_10880_cov_6.282771"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..2693
                     /gene="galB"
                     /locus_tag="PK252_12950"
     CDS             288..2693
                     /gene="galB"
                     /locus_tag="PK252_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HOT15660.1"
                     /translation="MMKTKQLLSIAIYLILSGMTVFSSEKTSFNKGWKFINKDTIASA
                     INFDDSRWRTLNLPHDWSVEGTFDEKNPSTPGGGALPGGIGWYRKTFTIEKADSGKLF
                     FIDFDGVYRNSEVWINGQYLGKRPNGYISFRYELTPYLHYGNTPNVIAVKVDNSKQPN
                     SRWYSGSGIYRNVWIIKSEKIRVDHWGTFIKTLKDSAGIAIIELDIKIKNTSGKSAQL
                     TVKSEIIDQKGIVVDNRKSKILSPNNAISGTKQLFEIEKPNLWSVENPYLYKVVTTVI
                     SGKKVLDTYETPLGIRTFTFDANKGFLLNGKKVKINGVCNHHDLGCLGAAINVRALER
                     QLEILKSMGVNGIRTSHNPPAPELLDLCDKMGFIVMDEAFDMWKKKKTEFDYSLDWDV
                     WHKQDLEDFVLRDRNHPSVFIWSIGNEILEQWDSTGITIGKELATIVRSLDATRPITS
                     ALNDPNPNNYIYQSGSLDLVGFNYHHQDFANFPKNFPNQKFIATETVSALATRGSYDM
                     PADTIKRWPQAWDKPLVGGNKDFSCSSYDNCSAPWGSTHEETWKIMKKNDFLSGQYIW
                     TGFDYIGEPTPYPWPARSSYFGIVDIAGFPKNAFYLYQSEWTSKPVLHIFPHWNWKAG
                     DTIDVWAYSSFRTVELFLNGKSVGKKTKGTDDLHLSWRVPFEAGTLKAIGYIGEKAEM
                     ATEVKTAGEPAKIILEADRQTIKADGSDLSFVTVKVVDKDGVVVPYADNQIQFSISGE
                     GTIVGTDNGSQISMEPFKSSSRKAFHGLCLAVVQSTEKQGQITLKATADKLPQAEIII
                     QSK"
     gene            2733..5906
                     /locus_tag="PK252_12955"
     CDS             2733..5906
                     /locus_tag="PK252_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15661.1"
                     /translation="MKKKEKFAKQWMPAMKKLLALFATSMLMCVTALSQERTVTGTVK
                     DASGTGLPGVNVIVKGSSNGVITDLDGKFSIKVPGDNAVLQVSFIGYKTEEVSVGQQN
                     SFAVTLNEDVKQMDEVVVVGYGVQKKKLVTGATVQVKSEDLTKNNVTRIESALQGLTP
                     GMSIVKQSGQPGSNYNITIRGLGSVNGSEPLVLIDGVPGSMSSVNPSDIETVDVLKDA
                     ASAAIYGSRAGNGVILITTKKGKTGKMQISYDGYYGISNPWKKVDVLNASQYMGIMNE
                     QQRNDGRTPYFSAEDSISNSVNTDWQDVVTNKNAPQQSHYLAITGGNEYSTFSISLSQ
                     NKEEGIFDFEGKSKFERLGFRINSDHKVKSYLQIGENLTYTHRDSRALGTGNQYNNFM
                     RQVLSASPLIKPYDETVYDGFGRSKNNKVKGLGVPYDGQINPIADMHYNYNGHTKNDD
                     IVGNIYAELEPVKGLKLRSDFGGTLTYSYYSEFNDTFHLTPYTYNTLQNVKQNVYRNF
                     GYNFDNTATYTKDIGNHNFLVMIGMNNQDNWFFNIIGERKGYLTSSAPILNNVSSNLK
                     LDTANGNFGKGDARISYFGRIAYNYNEMILFSASLRRDASSRFGANNRVGYFRAFSGG
                     FVLTKLDMFSTLEWLNFLKIRASWGENGVEPNRPYMFLATVNAANKTYAFGSGISPNI
                     VPNPALRWEASQQLDIGFESRFLNCLSFSFDYYVKTSKDWIIPVTVPGISGIAGINER
                     VNPYKNAGKVTNTGVEFDVTYAKNFGDFALELKGNLSYNENKVNEVPDSIIHGSSSVL
                     YNGSEEFCRVQEGMPMGFFWGYKTDGILQSKQEADEYNSKLKRQQAVPGDVRFVDTNG
                     DGILDQKDKVMIGDPHPDIIFGFGATASYKAWDFSMNISGQLGNQIVQCYRMQETAYG
                     NYTTEILDRWRWTDANANGIVDAGESNNSSRPRVTNGKETNKNWRNISDLYVYDGDFL
                     RVKSINLGHDLKKSLLKNIPVGQLRLYVSATNILTITKYNGIDPEVGYGSYYDNAGNL
                     TDAFASGIDMGFYPSAITYLVGVNVKF"
     gene            5919..7559
                     /locus_tag="PK252_12960"
     CDS             5919..7559
                     /locus_tag="PK252_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT15662.1"
                     /translation="MKKIHILLILAIAIMGACKEDTLDRYPTDASVIESYYKTPNDAT
                     MAVNAAYNMLLRDDWWSLYIYSEILSDNCAGGAGSGDGGGFQRLDRLLQWPEADAFQI
                     HWRTYYGAIYRTNIYLESEGLINWTGKESLRNQYKAEIRFLRAYYHFYLARMFGNIPA
                     LDHSLLPGEIPSRSTPEALYGLIIDDLKYCIENGLSTPYSQMTEANWGRATKWAAEAM
                     LARVFLYYTGYYNKTEINGINNNVVQTYLDDVIANSGHGLVDHYASLWRVPTYSELKS
                     IDLYAGEKNKEVVWSIRYNITGAGGNLYGASWWQRMIGPRNTNVDPYGNGWGAAPVLP
                     TTWNMYENGDTRKTATILSWADEGLTYDFTGAQQAQYTGYNLKKYDIASIEKRPEDVV
                     MGGTDWQTEAFEDYIVIRYSDVLLMAAELHLLNGDAATALNYVNQVRTRAFGNTEHNL
                     SSITLDAIYNERRLEFVGEGIRYYDILRYCKGDFSKLVNYLTYVDQNDGGDFSKSIDT
                     ESLDVDGNSFAEKKGLFQIPQAEINLMKGKIQQNEGYN"
     gene            7784..8986
                     /locus_tag="PK252_12965"
     CDS             7784..8986
                     /locus_tag="PK252_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOT15663.1"
                     /translation="MKKLLLIAAALASFSAFAQKNNDLAKTPPMGWNSWNTFACDINE
                     KLIKEMADVMVSSGMRDAGYVYLNLDDCWHGQRDSLGFIHPDPQRFPSGIKALADYVH
                     SKGLKIGIYSDAGWKTCGGKPGSRGYEFQDALTYAKWGIDYLKYDWCYTEGLNAEEAY
                     KSIGTALVASGRPIVLSICEWGNHKPWTWGAKYGHLWRTTGDIVDCWDCELGHGSWAS
                     KGILRILDMQIDSGIRQYAGPGHWNDPDMMEVGNDLTVSESRAHFSLWCMLAAPLIAG
                     NDLRSMTKETKEILTNKEMIAIDQDSLGVQCFRWFDYRTFQVFAKPLANGELAVCFLN
                     RGETPYNINFDWKDTENIYDGPHVYEFNKTTYKLRDVWAHKDAGTTAKPFVATLAPHD
                     VIVLRLKK"
     gene            9142..9780
                     /gene="pdxH"
                     /locus_tag="PK252_12970"
     CDS             9142..9780
                     /gene="pdxH"
                     /locus_tag="PK252_12970"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOT15664.1"
                     /translation="MMNIFELREEIAAEKLDEKSVPDNYLSLFTKWYKQAAEAKIYEP
                     GAFALATADKIGNPSCRMLLLKGFDKRGFIFFTNYDGRKATHLAENNHAAMVFYWGEI
                     ERQVRIEGIVEKISAEESDSYFETRPVGSRLGAWASPQSQPINSREWLEKAHNDFFEK
                     FNNKAIPRPENWGGYLLSPTLVEFWQGRPNRLHDRIQYSLDGLDWKIRRLAP"
     gene            9949..10758
                     /locus_tag="PK252_12975"
     CDS             9949..10758
                     /locus_tag="PK252_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOT15665.1"
                     /translation="MKTLFFLFLLIPIIVQGQKKTDEEASWSESLNKCKAAILTIDNA
                     NITIRGNDKKSPQLNLLVKRNDKVIPLKQEEIDKNFDLKISYKHDSLFFDCTRKMEEN
                     KGVFKEGTVIEFSITLNVNTKIDIKLEKGDINIKNIKTSAIKINNADGNMKILESGGF
                     ANLIASDGDIIFSSHNGGMVAETQNGKIQGDFENITDSIQMENNNGFINIIVPKETKA
                     RIFAKGKLASTNTSMIQGTIARTLISGSLNDGAMPIRLNTPKGNISFFVKE"
BASE COUNT         3552 a         2227 c         2250 g         2851 t
ORIGIN      
        1 aagccgcaag tttaatgttg tccttattag tgaaaacaag ccttacaata ttggacaaaa
       61 gttgattaac ccaattgaag tagaatacat aggtgatgaa ttaaaaatta gtttgaaata
      121 gtggtattta gagaaatgcc atttatgttt ctcatatagt tagttaggtt gtgcaattgt
      181 gaaaaagagc ctgtgaaaag acaatgcaaa aagaaaggaa aagctcttgg tgctttctgt
      241 agtaacatac agagggcact tcacagttgt caaaaattaa aactcaaatg atgaaaacaa
      301 aacaactatt atctattgct atatacctta tcttatcagg catgacagtt ttttcgtcag
      361 aaaaaaccag ttttaacaaa ggatggaaat ttattaataa agataccata gcgtcagcaa
      421 tcaattttga tgacagtcga tggcgtacac ttaatctgcc gcacgattgg agtgttgaag
      481 gaacatttga tgagaaaaac ccatccactc cgggaggagg tgctttgccc ggcggaattg
      541 gctggtaccg caaaactttt accatcgaaa aagcagatag cggaaagcta ttttttatag
      601 attttgatgg agtgtacaga aacagtgaag tttggataaa tggccaatat cttggaaaaa
      661 ggcccaatgg ctacatatca ttcaggtatg agcttacccc ctacttacac tatggcaaca
      721 cgccaaatgt tatagccgta aaagttgaca actccaaaca accaaactcg cgttggtatt
      781 caggctccgg gatataccgc aatgtatgga taataaaatc ggaaaaaata agggttgacc
      841 attgggggac ctttataaaa acattaaaag actcggcagg catagccata atagagttag
      901 atataaaaat aaaaaacact tcgggcaaaa gtgcccaact aactgttaaa tcagaaatta
      961 ttgaccaaaa agggattgtt gttgacaaca gaaaatcaaa aatactctct ccaaacaacg
     1021 ctatttctgg cactaaacag ctttttgaaa ttgaaaagcc caacctgtgg agcgttgaaa
     1081 acccttacct ctacaaagta gtaactacag tcatttcagg aaagaaagta ctagacacgt
     1141 atgaaacccc gcttggaatc aggactttta cattcgatgc taataaagga tttttgctca
     1201 atggcaaaaa agtgaagata aatggcgttt gcaaccacca cgacctcgga tgccttggtg
     1261 cagctattaa tgtaagggct ctcgagcgtc agttagagat tttaaaaagc atgggagtaa
     1321 atggcatacg cacatcgcac aacccaccag cccccgagct gcttgacctt tgcgacaaaa
     1381 tgggttttat cgtcatggac gaagcttttg acatgtggaa aaagaaaaag accgaatttg
     1441 attactcgct cgattgggat gtatggcaca aacaagattt ggaggacttt gtacttcgcg
     1501 accgcaacca cccgtcagtg tttatttgga gtatagggaa cgaaattctt gaacaatggg
     1561 attcgacagg catcaccata ggcaaagaat tagcaacaat agtacgcagt ttagatgcta
     1621 cccgccctat aacctcagcc ctcaatgacc ctaatcctaa taattatata tatcaatctg
     1681 gttctttgga tttagttggc tttaactatc atcatcagga ttttgccaac tttccaaaga
     1741 actttcctaa ccaaaagttt attgctaccg aaacagtatc agccctggct acacgcggaa
     1801 gttatgacat gcctgccgac acaataaaac gttggcctca agcctgggat aagcctttgg
     1861 ttggtggcaa caaagacttt tcatgttcgt catacgacaa ttgcagcgcc ccttggggct
     1921 cgacccatga agaaacatgg aaaataatga aaaaaaatga ttttctttcg ggacaataca
     1981 tctggaccgg gtttgactac attggcgaac caacaccata tccgtggcct gcacgcagct
     2041 cgtatttcgg aattgttgat attgcagggt ttcccaaaaa tgcattttac ctgtaccaga
     2101 gcgaatggac ttcaaaaccc gtattgcaca tatttcccca ttggaactgg aaagctggcg
     2161 acaccataga tgtatgggct tatagcagtt ttaggacagt agaactattc ctcaacggca
     2221 aatctgttgg taaaaaaaca aagggtacag atgacctgca cctgtcttgg cgtgtacctt
     2281 ttgaagccgg caccttaaaa gctattggat acattggtga aaaggcagaa atggccactg
     2341 aagttaaaac agccggtgag ccagcaaaga taatacttga agctgaccgt caaacgatta
     2401 aagccgatgg ttcagacctt tcttttgtca ccgttaaagt agtggataaa gatggcgttg
     2461 tagtgcctta tgccgacaat caaatccaat tcagcatcag cggtgaaggt actattgtgg
     2521 gtaccgacaa tggaagccaa ataagcatgg aaccttttaa aagcagcagc cgaaaagcct
     2581 ttcatggttt atgccttgcc gtagtacaat caaccgaaaa gcaggggcaa ataacactaa
     2641 aagccactgc agacaaattg ccacaagccg aaattataat ccaatcaaaa taaaaagcta
     2701 actaattatt attcaactat taactaaatt ttatgaaaaa aaaagaaaaa tttgcaaaac
     2761 agtggatgcc ggctatgaaa aagctgctgg cgctgtttgc aaccagtatg ttaatgtgcg
     2821 taacggcgct atcgcaagaa cgcactgtta ccgggactgt taaagatgcc agcgggacag
     2881 gcctgccagg tgttaatgta atagtcaaag gctccagcaa tggcgtcatt accgaccttg
     2941 atggaaaatt ctctataaaa gtaccgggag acaatgcggt tttgcaggtt tcatttatag
     3001 gatacaaaac agaagaagtc agtgttggcc agcaaaactc ttttgcagta acattaaatg
     3061 aagatgttaa gcaaatggac gaggtagttg ttgtaggtta cggcgtacaa aaaaagaaac
     3121 ttgttacagg cgctaccgta caagtaaaaa gtgaagacct gacaaaaaac aacgtaaccc
     3181 ggattgaatc tgcgttacaa ggtttaaccc cgggtatgtc tattgttaag caatctggcc
     3241 aaccaggctc aaattacaac atcactatcc gtggtttggg ctcggtaaac ggctcagaac
     3301 cccttgttct tatagacggg gttccaggaa gtatgagcag cgtaaaccca tctgatatcg
     3361 aaactgtaga tgtactaaaa gatgcagcat cagctgcaat ttatggttca cgtgcaggta
     3421 atggcgttat ccttatcact actaagaaag ggaaaacagg caaaatgcaa atcagctatg
     3481 acggttatta tggcatttca aatccatgga aaaaagtgga cgtactcaac gccagccaat
     3541 acatgggcat catgaacgaa caacaaagaa atgacggaag aacaccatat ttttcggctg
     3601 aagacagcat aagcaatagt gttaacactg actggcaaga tgtagtgaca aacaaaaacg
     3661 ccccccaaca aagccactat ttggctatca ccggaggtaa tgaatattct accttttcta
     3721 tctctctttc gcaaaacaaa gaagaaggca tctttgattt tgaaggtaag tcaaaatttg
     3781 aacgcttagg attccgcatc aactctgacc acaaagtaaa aagctatttg caaattggcg
     3841 aaaaccttac ctacacccac agagatagca gggcgctggg cacaggcaat caatataaca
     3901 actttatgcg acaggtatta tcagccagtc cgctaatcaa accttatgac gaaactgtat
     3961 atgatggctt tggcagatca aaaaataaca aagtaaaagg gttgggtgta ccttatgacg
     4021 gacaaataaa ccctattgct gatatgcact acaactacaa tggacatact aaaaatgacg
     4081 atattgttgg aaatatctat gcagaacttg aacctgttaa agggttaaag ctacgttcag
     4141 attttggagg aacgttgacc tactcatatt acagcgaatt caatgatacc tttcatctca
     4201 cgccttatac ttacaatacc ttacaaaatg taaaacaaaa cgtatatcgc aactttggat
     4261 acaattttga caatactgcc acttacacca aagacattgg aaaccacaat tttcttgtaa
     4321 tgattggtat gaacaaccag gataactggt tttttaatat tattggcgaa cgtaaaggct
     4381 acttaacatc atcagctccc attttaaaca atgtttcgag caacctaaaa ctcgataccg
     4441 ccaacggaaa ctttgggaaa ggtgatgccc gaatttcgta ttttggacgt atagcgtaca
     4501 attacaatga aatgattttg ttctcagcct cactgcgccg cgatgcatcg tcaaggtttg
     4561 gggcaaacaa ccgtgtaggt tatttccgcg ccttctcagg aggttttgtt ttgaccaaat
     4621 tagatatgtt ttctactctt gaatggctca actttttaaa aatacgggca agctggggag
     4681 aaaatggggt tgaacccaac cgtccataca tgtttctagc tactgttaat gctgctaaca
     4741 aaacctatgc atttggctca ggtatttcgc ctaatattgt ccctaaccct gcattaagat
     4801 gggaagccag ccaacaactt gatataggct ttgaatcgcg tttcctaaac tgtctgagct
     4861 tttcttttga ttattatgta aaaaccagta aagattggat aataccggta actgttccgg
     4921 gcatatcagg tattgccggc ataaatgaaa gggtaaaccc atacaaaaat gccggaaagg
     4981 ttactaatac aggcgttgaa tttgatgtta cctacgccaa gaattttggg gattttgccc
     5041 ttgagttaaa aggaaacctc tcatacaacg agaataaagt aaatgaagtc cccgatagta
     5101 ttattcatgg ttcatccagc gtattataca acggtagcga agaattttgc cgggttcagg
     5161 aaggtatgcc tatggggttt ttctggggat ataaaacaga tggtattttg cagtcaaaac
     5221 aagaagccga tgaatataac agcaaactga aaagacaaca agctgtaccc ggagatgtaa
     5281 gatttgttga taccaatggt gatggtatcc tcgaccaaaa agataaagta atgattggcg
     5341 accctcaccc ggatattatt tttggttttg gcgccacagc ctcctacaaa gcatgggatt
     5401 tctccatgaa tatttcagga caacttggca accaaattgt acaatgctac cgtatgcaag
     5461 aaacagccta tggtaactat accacagaaa tccttgaccg ttggagatgg acagatgcca
     5521 acgcaaatgg aattgtagat gccggagaaa gcaataattc aagcaggcca cgtgtgacaa
     5581 atggcaaaga aaccaacaaa aactggagaa acattagcga tttgtatgtt tatgatggcg
     5641 atttccttcg ggttaaaagc atcaacttag gacacgacct taaaaaatca ttgcttaaga
     5701 atatccctgt tggacaatta agactttatg tttcggcaac taatatctta accatcacta
     5761 aatacaatgg tattgatcca gaagtgggtt atggctctta ttacgataac gcaggcaacc
     5821 taactgacgc atttgcttct ggtatcgata tggggtttta tccttcggca ataacctatt
     5881 tagtgggtgt aaatgttaaa ttttaaataa gaaaaaagat gaaaaaaata catatcctat
     5941 tgatattggc aatagcaata atgggagcct gcaaagaaga tacccttgac aggtatccta
     6001 cagatgcctc tgttattgaa tcgtactaca aaactcccaa tgacgcaacc atggcagtaa
     6061 atgcagccta caatatgtta cttcgcgatg actggtggag tttatatatt tactccgaaa
     6121 ttttgtcaga caactgtgct ggcggggccg gtagtggtga cggaggtggg tttcaaagat
     6181 tagaccgcct gctgcaatgg cctgaagccg atgcttttca aatacactgg cgtacatact
     6241 acggtgcaat ttatcgcaca aatatctacc tcgaaagcga gggattaatc aattggactg
     6301 gtaaagaatc gcttcgtaac caatacaaag ctgaaatccg ttttttgcgg gcatattatc
     6361 acttctacct tgcccgtatg tttggaaata ttccggcatt agaccatagc ttgttacctg
     6421 gtgaaatccc ttcaagaagt acccccgaag ctctttatgg cttaatcata gacgacctga
     6481 aatattgcat tgaaaacgga ttatctactc cttatagcca aatgaccgaa gcgaactggg
     6541 gcagggctac aaaatgggca gccgaagcta tgcttgcaag agttttctta tattacacag
     6601 gctattacaa caaaaccgaa atcaatggca tcaacaacaa tgttgtacaa acttaccttg
     6661 acgatgttat tgccaatagc ggacatggtt tggttgatca ttacgccagc ttgtggcgtg
     6721 tacctacata ctctgaatta aaatcaatag acctttatgc cggcgaaaaa aacaaagaag
     6781 tagtttggtc catccggtac aatataaccg gagcaggagg caatttatat ggagcttcat
     6841 ggtggcaacg catgattggg ccacgtaata ccaacgttga cccttacggg aacggatggg
     6901 gagctgcccc tgtactccct acaacatgga atatgtatga aaatggcgat actcgtaaaa
     6961 ctgctactat tttatcatgg gctgacgaag gacttactta tgatttcacc ggtgcccaac
     7021 aagcccaata cacagggtat aacctcaaaa aatacgatat tgccagtata gaaaaaagac
     7081 ctgaagatgt agtcatgggc ggtaccgact ggcaaaccga agcattcgaa gattacatag
     7141 taatccgcta ttcggatgta ttgcttatgg ctgcagaatt acaccttttg aatggcgatg
     7201 ctgctacggc cctcaattat gtcaaccagg tccgtacacg cgcctttggt aataccgaac
     7261 acaacctgag ttctatcacc ctcgatgcta tctataatga acgacgttta gagtttgttg
     7321 gagaaggtat tcgttactat gatatccttc gctattgcaa aggtgatttc tcaaaacttg
     7381 tcaattacct tacctatgtt gaccaaaacg atggaggaga tttctcaaaa tcaattgata
     7441 ctgagagttt agacgtagat ggtaatagtt ttgccgaaaa gaaagggcta tttcaaatac
     7501 cacaagctga gattaacctg atgaaaggta aaattcaaca aaacgaagga tataattaag
     7561 aagttcaaca atttacaaac aaccacctat tttaaaaaac ccttgggagc ttttcccggg
     7621 ggttttattt taagcacaaa accttccagg ttttcgaaaa cctggaaggt tattgcttga
     7681 actacctaac atatccactt tataacttaa cctgctttta aagtgtgcat ctcacttttt
     7741 taaagtaaaa ttgcccttag ttcaaatttt taaaccaact aatatgaaaa aattattgtt
     7801 aattgctgct gcattggcct ctttttcagc ttttgcacaa aaaaacaacg acctggcaaa
     7861 gactccgcct atgggctgga acagctggaa cacctttgcc tgtgatatta atgaaaaact
     7921 tatcaaggaa atggcagatg ttatggtttc atcgggcatg cgtgatgccg ggtacgtata
     7981 tctcaacctc gacgactgct ggcatggaca acgcgacagc cttggcttta tccatcctga
     8041 tccacaacgc ttcccatctg gaataaaagc attggcagac tatgtacatt caaaaggcct
     8101 taaaataggc atatactctg atgcaggatg gaaaacctgc ggaggcaagc ccggcagccg
     8161 tggatatgag tttcaggatg cgctaacata cgctaaatgg ggcattgact atttgaaata
     8221 cgactggtgc tataccgaag gcttgaatgc agaagaagca tataaatcta taggtacagc
     8281 tcttgttgct tctggcaggc caattgtgct aagtatctgc gaatggggca accacaaacc
     8341 ctggacatgg ggggcaaaat atggccactt gtggcgtact accggcgaca tcgttgactg
     8401 ctgggactgt gagctgggac atggcagttg ggcttcaaaa ggaatattac gcatactcga
     8461 tatgcaaatt gatagcggaa tacgacaata cgccggcccg ggacactgga acgaccccga
     8521 tatgatggaa gttggcaatg accttactgt cagcgaaagc cgtgcccact tttcgttgtg
     8581 gtgtatgctt gcagcccctc tcattgctgg caatgacctt cgttcgatga ctaaagaaac
     8641 aaaggaaata ttgaccaaca aagaaatgat tgctatcgac caggattctc ttggggttca
     8701 atgcttcaga tggtttgatt accgcacttt tcaggtattt gccaaacctc ttgccaatgg
     8761 agaactggct gtttgcttcc tcaaccgtgg cgaaacccct tacaacatca actttgactg
     8821 gaaagatacc gaaaacatct atgatggccc acacgtttac gagttcaaca aaacaaccta
     8881 taaattaagg gacgtttggg cacacaaaga cgctggcact actgccaaac cttttgttgc
     8941 aacccttgcc ccacatgatg taatcgtgtt aaggttaaag aaatagtttg aaatattggt
     9001 tttgtaagag cgacaggttt gaaaaagctt gtcgctcttt tgttttctca tctcgaaacc
     9061 ttctaggttt taaaaacctg gaaggtttga accagctata aactttccta taaattgttt
     9121 gtttacccaa aaaccttatt gatgatgaac atttttgagt tacgagaaga aatagccgcc
     9181 gaaaagctgg atgaaaagag tgtccccgat aattatcttt cactttttac caaatggtac
     9241 aaacaggctg ctgaagccaa aatatacgaa cccggtgcgt ttgcattagc tacagcggac
     9301 aaaatcggaa acccttcatg ccggatgctt ttgctcaaag gctttgacaa acggggattt
     9361 atcttcttca ccaattatga cggacgtaaa gcaactcatt tagctgaaaa caaccacgct
     9421 gctatggttt tttattgggg cgaaattgaa aggcaggtca ggattgaagg gattgtagaa
     9481 aaaataagtg cagaagaatc tgacagctac tttgaaaccc gtccggtagg ttcacgcctt
     9541 ggagcatggg cttcacctca aagccaaccc atcaacagcc gcgaatggct tgagaaagca
     9601 cacaacgatt tttttgaaaa gttcaataat aaagccattc cccggcccga aaactgggga
     9661 ggataccttt tatcgcccac tttggtagaa ttctggcagg gccgccccaa ccgcctccac
     9721 gaccgtatcc agtattccct tgatggactt gattggaaaa taaggcggtt ggctccatga
     9781 gggtgtgtaa aagtacaatt ttactcattt ttcgactctg cccaaaatga caaactgctg
     9841 attttcaggc gtcaagctga gcgtagtcga agctttgtct gactttttac acaccttttt
     9901 tcatttctcc cacttattcc ctaactttgg ataaataata ccaaagaaat gaaaacacta
     9961 ttcttcctgt tcctgcttat tcctattatc gttcagggac aaaaaaagac agacgaggaa
    10021 gcctcctggt cagaaagcct caacaaatgc aaagctgcca tcctgactat cgacaacgcc
    10081 aatatcacca tcaggggcaa tgacaagaaa agccctcaac taaacctttt agtaaaaaga
    10141 aatgacaaag taatccctct caagcaagaa gaaattgata agaatttcga tttgaagata
    10201 agttataagc acgactcgct atttttcgat tgtacacgaa aaatggagga gaacaaaggt
    10261 gtttttaaag aaggtacggt aatcgagttc tccattacac ttaatgtcaa tacaaagatt
    10321 gatattaagc tggaaaaagg cgatataaac atcaaaaaca tcaaaacctc ggctatcaaa
    10381 atcaacaatg ccgatggcaa tatgaagata ctcgaaagtg gaggctttgc caacctcatc
    10441 gcctcggatg gcgacatcat ctttagcagc cacaatggcg gcatggtagc cgaaacgcaa
    10501 aatggcaaaa ttcagggcga ctttgaaaat attaccgaca gcatccagat ggagaacaac
    10561 aacggtttca tcaacatcat tgtgcccaaa gagaccaaag cacgtatatt tgccaaaggc
    10621 aaacttgcca gtaccaatac cagcatgata cagggcacca ttgcccgtac cctgatatca
    10681 ggctccctca acgatggagc tatgcccatc aggttgaaca ccccaaaagg aaatatttcg
    10741 ttttttgtaa aagaataaaa gtttgaaaac aattaagctc agaaagttca gccaagacga
    10801 aagccatgtc ttaaaaggta ttgctatgag agtataaaat attttgtgta aacacaataa
    10861 tttgattttt aatttatttt
//
LOCUS       DAOSPF010000089        10819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6073_length_10819_cov_6.371981, whole genome shotgun sequence.
ACCESSION   DAOSPF010000089 DAOSPF010000000
VERSION     DAOSPF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_10819_cov_6.371981"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK252_12980"
     CDS             complement(<1..227)
                     /locus_tag="PK252_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HOT15666.1"
                     /translation="MNDAAKKKYWKEYQKDIANDDYYYARSCIRQNFFPGSEETFLKI
                     LREVLGKNVFDDSHQTTCTGIAYHSSIVPQQT"
     gene            complement(220..828)
                     /locus_tag="PK252_12985"
     CDS             complement(220..828)
                     /locus_tag="PK252_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT15667.1"
                     /translation="MFTDKLKQDVRFIEGLKACINCGTCTAICPAAQFYNYDPRQIAD
                     TVQNGNDTEIEQLLKSDTIWYCGECLSCKTRCPRDNTPGYIVQALRSLSIKEGYYLLS
                     EKGRQQLEIKRRVGDIMLKYGYCVYVDEIETGYHPEQGPIWDWYRANTESILKKLGAN
                     YKGSGAGTLRKIDKADMDELHKIFEETGAMQQFNKLEDEGHE"
     gene            complement(875..2569)
                     /locus_tag="PK252_12990"
     CDS             complement(875..2569)
                     /locus_tag="PK252_12990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT15668.1"
                     /translation="MKPKRIIVIGGSAAGPKAASKARRMDENAEITMYQKASDLSMAS
                     CGYPYYIGGFFDDRNQLLCSPAGVVRDSKFFWNAKKIVTKVNTEVTAIDRKNKRIEFI
                     DLVSGEKGSAEYDKLVIATGATARKPPIPGVELEGVTTLQSLPDADYLRKVHDEGKIK
                     KAVVIGGGLIGIETCEALNLAGIEITLIELLPQLLTFLDRQMAKLVEKYVQSKANVIL
                     ENGVAAFIGENGKLTAVKLQNGTEIPCQLAVVAIGVVPNTKLAKDIGLNIGKTGGIEV
                     NEYMQTSDPDIYAVGDCIEIPNLITQHAVHAPYGDLANLQGRVAGQNVILGNIAKFPG
                     TIQTGICKVFDYGVGATGLSEQNAAKNGIKNIETVINASLDKPGFMQGKLLVTKLVVD
                     KSNGKILGAQVLGPGDVSKQVAIWAMAIKGNLTVNDMVNADLPYAPPYSLAIDHSIAT
                     AHIMQNKLNGYFTGISAEQLKEKLDKKKPLVLLDFRNPDEYEQMRLGIGEKLIPLGQL
                     RKRMDEMPQDKNAEIICWCKISLRGYEAALILQANGYTNVKVLEGGIMAWPYSREK"
     gene            complement(2574..3314)
                     /locus_tag="PK252_12995"
     CDS             complement(2574..3314)
                     /locus_tag="PK252_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOT15669.1"
                     /translation="MITLIILAVLLGNIGSVLMASLMLKFKNEYLQKLTKHINAFAGG
                     TLLGAAFLGMLPNAIKIAGYQISYAVLAGILFFFIIEKIMLWRICADENCDRHSKAGA
                     LMLMIGDSFHNFLDGIVIAAAFLHSPSFGIVVAISVFAHEIPQEVADFGVMLKAGFSK
                     RKAVLYNLLSGLTAIVGALLAWYAGLYTQIFMPFILALSAAGFIYIALADLVPQMHRK
                     TNIRQSVFQFLLLIAGISVIIISIITKP"
     gene            complement(3397..3834)
                     /locus_tag="PK252_13000"
     CDS             complement(3397..3834)
                     /locus_tag="PK252_13000"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOT15670.1"
                     /translation="MTRKEAIDMAKGLFDEGYACSQSVLLTFSKEFELDERTAKLISS
                     TFGGGMGRLRQKCGAVTGGFMVLGLKYGNEMPKDMETKLHAYKKVRELNSMVEDIYGT
                     SNCFELLKKHATEADVEARKHHKIICRSVVGDVAGLVYDMINK"
     gene            complement(3839..4264)
                     /locus_tag="PK252_13005"
     CDS             complement(3839..4264)
                     /locus_tag="PK252_13005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT15671.1"
                     /translation="MIEHVYTFRVMYPETDQMGTVHHSNYAKYYETARWELFRSIGIS
                     YHSVEEAGYMLPVISMNFKFLKTTHYDEQLTVKTTLKAIKGVRIWFTYKLYNEQNELI
                     NEAETELAFVGRDNWKPCAAPDFVLKAIEKCCLQAITNN"
     gene            complement(4270..5061)
                     /locus_tag="PK252_13010"
     CDS             complement(4270..5061)
                     /locus_tag="PK252_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT15672.1"
                     /translation="MNQIAKTKLPNIKNIIIVASGKGGVGKSTVAAGLAMSLAMEGYA
                     TGLLDADIYGPSVPTLFNLKGEKPSVFEKNGKQYMEPFVKFGIKLMSIGFFIDNKQAV
                     IWRGPRVSSGITQLLTDTSWGELDYLIIDTPPGTGDIHITLLQNFETSGAIVVTTPQN
                     MALADVKKAVDMYNDKFIGTPVLGIVENMAWFTPSQHPDEKYFLFGKDGGNVLSKEFN
                     IPLLAQIPINEKLCEVCDNGALHELFDDIAIKTAFGQLLINITKN"
     gene            complement(5092..5508)
                     /locus_tag="PK252_13015"
     CDS             complement(5092..5508)
                     /locus_tag="PK252_13015"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT15673.1"
                     /translation="MKYIISKEFSFEAAHRLNKNYTGKCTNNHGHSWLVRVSIEAETL
                     DNKDMVIDFQDTKVLKQWIDEKLDHATILWEHDPMCQYIRDTGQRLFVTSKNPTSEHL
                     AEIILEKAQQFFDKGNTRVHCIELNETCTNGAKLFP"
     gene            complement(5529..6404)
                     /locus_tag="PK252_13020"
     CDS             complement(5529..6404)
                     /locus_tag="PK252_13020"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15674.1"
                     /translation="MTEIAILSGKGGTGKTSLSAAFATIGNNIVVADCDVDAANLHLV
                     LQPVNDKEEVYISGYRAVINYDICASCGQCEAYCRFDAISMSNGKVFISDTSCDGCKL
                     CSRICPVQAITMVPSDKSRWYIGNYRNGRMVHARLAPGEENSGKLVNLVREQARNVAN
                     EIDSNIIIIDGPPGTGCPVISSVTGVNKVVVVTEPSMSGFHDMKRVLEIVANFKAKPY
                     VVINKYDLNPNISGQIEAWCIQQNIAIVGRLSFDENVVNAMVNCKSIIEYAPQSEISK
                     EIFKIYEKVFCSLNI"
     gene            complement(6401..7255)
                     /locus_tag="PK252_13025"
     CDS             complement(6401..7255)
                     /locus_tag="PK252_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15675.1"
                     /translation="MNIAVASGKGGTGKTLVSTNLFHALQKRGVTVILVDCDAEEPNV
                     MEFIQGETFASNVVTQNIPVIDTPKCTFCGKCYEYCNYNAILYLQPSKYIQVVEDLCH
                     DCGACSVACLYGAITEKPKTVGKVDSVYLSCHSQLIEGCAEVGVYSPVPVIKRAIKEA
                     QANTLNIFDSPPGISCPFIATVDKADYVILVTEPTPFGLNDLKLSIETLQQIGKPFGV
                     VVNRSTLGNSDVYNYLEQNSFTLLMEIPFDKEIARIYSEGKMLVDEKPEYQERFIALY
                     QLVKKQIQ"
     gene            complement(7252..8109)
                     /locus_tag="PK252_13030"
     CDS             complement(7252..8109)
                     /locus_tag="PK252_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOT15676.1"
                     /translation="MISDYRCFFCFTKAYERLLEEGTLSASAKDNFTRDMAALYLKHC
                     ERFSAPYFSRDLHIALNKYTNNPDPHREGKKLSNDLVLAMYNELKEKVQTSVNPFDTA
                     LRLAIAGNIIDFAVSKNYDLHATIDKVLYSHFAIDHSAELKKAIAGAQTILYLGDNNG
                     EIVFDKLFIETVGHPDVYFAVRGAPVINDVTLDDARYVGIDKVAKVISNGYDAPSTIV
                     EHCSDEFRHIFEKADIVISKGQGNLEGLLHRTQKEVYFMLMVKCNVIADALQVNKGDF
                     VVKKNSLER"
     gene            complement(8121..8483)
                     /locus_tag="PK252_13035"
     CDS             complement(8121..8483)
                     /locus_tag="PK252_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT15677.1"
                     /translation="MKKKIAIPMENGVLSAHFGHCQQFAIVDVENNQITSVTEVTPPE
                     HVPGLYPRWVAEFGVTDVIGGGMGQKAIDLFHQQNINVFIGAPIKAPNELVREFIDNK
                     LSLTANYCNHDHQHGCDH"
     gene            complement(8522..8755)
                     /locus_tag="PK252_13040"
     CDS             complement(8522..8755)
                     /locus_tag="PK252_13040"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT15678.1"
                     /translation="MPGLNRRGPNGEGPMTGRKMGRCNPDNKGKTEEEIFQNRNMNNR
                     EQDTTFGQGWGRGKGRGMGKGPGRGLGRRFGQV"
     gene            complement(8812..8994)
                     /locus_tag="PK252_13045"
     CDS             complement(8812..8994)
                     /locus_tag="PK252_13045"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HOT15679.1"
                     /translation="MAHLDGKGPEQKGPKTGRKLGQCRVINQDEALDKLGKGMGLRRK
                     SGGGQGLGKRLKSGLK"
     gene            complement(9031..9360)
                     /locus_tag="PK252_13050"
     CDS             complement(9031..9360)
                     /locus_tag="PK252_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT15680.1"
                     /translation="MKVAITSTGNSLESTLDSRFGRCSYFVIYDTKTQATEFIPNPNL
                     ESVEGAGPASVNLIASRGVKKVVSGEFGTKVKSIFDSLQIQLVVVNDPKKTIAEIIEL
                     LEKGKEE"
     gene            complement(9373..9948)
                     /locus_tag="PK252_13055"
     CDS             complement(9373..9948)
                     /locus_tag="PK252_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOT15681.1"
                     /translation="MPRPKRIRKMTNPPHFRGYKPIGLSEESTPVILNYEEYEAIRLS
                     DFELYGQVEAAQIMDVSRPTYARIYESARRKVAQAFVLGKVIVFEGGKVYFDSEWYYC
                     HFCGCYFNHPAKDTEVNACALCGSKNIEQNSDSPEQANTEDICTCPKCGKEKEHTSGI
                     PCQKEICPECKCQMVRKGAPHHHRMRNNNCQ"
     gene            10045..10755
                     /locus_tag="PK252_13060"
     CDS             10045..10755
                     /locus_tag="PK252_13060"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT15682.1"
                     /translation="MEENQAMSMPRIGESAPEFKAVTTQGNINFPADFKGSWVILFSH
                     PADFTPVCTSEFMTFASMESKFAEVNCKLVGLSVDGLYSHIAWLRTIKEKISYKGMKD
                     VEVKFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFFIDPKGIIRTIIYYPLSLGRNF
                     DELYRVVVALQAADSFGVAMPADWRPGDDVIVPTAGSCGVAKERMDGKEEGVTCHDWF
                     FCTKKIAEDKVWAAVKKK"
BASE COUNT         3029 a         2415 c         2062 g         3313 t
ORIGIN      
        1 gtttgctgag gcactatgct cgaatggtat gcaatacctg tgcatgtagt ttggtgtgag
       61 tcgtcaaaca catttttgcc cagcacctca cgcaatatct tcagaaaagt ttcttctgag
      121 cccgggaaaa aattctggcg gatacagcta cgggcatagt aatagtcgtc gttagcaata
      181 tctttctggt attctttcca gtactttttt ttcgcagcat cattcatggc cttcgtcctc
      241 caatttatta aactgttgca tagccccggt ttcttcaaat attttgtgca gttcgtccat
      301 atcggcctta tcaatcttgc gcaacgtccc tgcaccagaa cctttatagt ttgcaccaag
      361 ctttttcagt atgctttcgg tatttgcccg gtaccaatcc catataggac cttgttcggg
      421 gtgatagccg gtttctatct catccacata cacacagtat ccgtatttaa gcatgatgtc
      481 gcctacacgc ctttttatct cgagctgttg acggcctttt tcagaaagca aatagtaacc
      541 ttcctttata gataagctac gcaacgcctg gacaatgtac ccgggagtat tgtcgcgcgg
      601 acaacgcgtc ttgcacgaga ggcattcgcc acagtaccaa atggtatcgc tttttaagag
      661 ttgctcgatt tcggtatcat taccattctg cacagtatca gctatctgtc gcgggtcata
      721 gttgtaaaac tgggcagccg gacaaatagc agtgcacgtg ccgcagttga tgcaggcttt
      781 aaggccttcg ataaagcgga catcttgttt tagcttatct gtaaacattg tttagcatta
      841 aataaacaaa ggtattaaaa cataaatacg atttctattt ttcacgagaa taaggccagg
      901 ccataatgcc tccttccaaa acttttacgt ttgtatagcc gtttgcctga aggattaaag
      961 cagcttcgta accacggagc gatattttgc accagcaaat gatttcagca tttttgtcct
     1021 ggggcatttc gtccatgcgt ttacgaagtt ggccaagagg gataagcttt tcgccaatgc
     1081 ccaagcgcat ttgttcgtat tcgtcggggt tacggaagtc gagcaaaaca agcggtttct
     1141 ttttatcaag tttttcttta agctgttctg ccgatattcc tgtaaaatat ccgtttagtt
     1201 tattttgcat aatatgtgcc gtagcaatgc tgtggtcgat agccagcgaa tacggtggtg
     1261 cataaggaag gtcggcattt accatatcgt ttacggtaag attgccttta atggccatag
     1321 cccagattgc aacttgcttg ctcacatctc ccgggccaag aacctgcgcg cctaaaattt
     1381 taccgttcga tttatctaca accaatttgg taaccaatag ctttccctgc ataaacccgg
     1441 gcttgtcgag actggcattt ataacggttt cgatgttttt aataccattt ttagctgcat
     1501 tttgttcgga taagccggta gctccaacac cataatcgaa caccttacat ataccggttt
     1561 ggattgtgcc gggaaatttg gctatattgc ccaaaataac attttggccg gcaacacgcc
     1621 cctgcaaatt agccaaatcg ccataaggcg catgaacagc atgttgagta ataaggtttg
     1681 gtatttcaat acaatcgcca acggcataaa tatcaggatc agaggtttgc atgtattcgt
     1741 tgacttctat acctccggtt tttccaatat tcaagcctat atctttggcc agttttgtat
     1801 ttgggacaac accaatagct acaaccgcca attgacaagg aatctcagtg ccattttgca
     1861 gcttaacagc agtaagcttg ccattttcgc caataaaagc ggcaacaccg ttttcaagaa
     1921 ttacattggc tttgctttgc acatattttt ctaccaactt ggccatttgc ctgtcgagaa
     1981 aagtgagcaa ctgaggtaat agctctataa gggttatttc gatacctgcc aggttcaggg
     2041 cttcgcatgt ttcaatgcca ataagccctc ctccaataac cacggctttt tttattttgc
     2101 cttcatcatg gactttacgc aggtaatcgg catcgggcaa cgattgtaaa gttgttaccc
     2161 cttcgagctc tacgccaggt ataggcggtt tgcgggctgt agccccggtg gcaataacca
     2221 gtttgtcgta ttcggccgag cctttttcgc cactgaccaa atcgataaat tcaatacgct
     2281 tattttttcg gtcgatagca gtaacctcgg tatttacttt ggtaacaatc ttttttgcat
     2341 tccaaaaaaa tttagagtcg cgcactactc cggcagggga acataaaagc tggttacggt
     2401 catcaaaaaa gccgccgatg taatacggat acccgcacga ggccatagaa aggtcagatg
     2461 ctttctgata catagttatt tcggcgtttt cgtccattcg gcgtgctttt gacgctgctt
     2521 taggaccggc tgccgaaccg cctattacga taatccgttt aggtttcatc atactatggt
     2581 ttagtgatta ttgaaataat tatgactgaa attcctgcta taagaagcaa aaattgaaat
     2641 acagattgcc ggatatttgt tttccggtgc atttgaggga ctaagtcggc aagggctatg
     2701 taaatgaaac cggctgctga caaagcaaga ataaaaggca tgaaaatttg ggtgtataac
     2761 cctgcatacc atgccaacaa agcccctaca atggcagtta accctgataa aagattataa
     2821 aggacagctt tacgtttaga gaaaccagct tttaacatta ccccaaagtc ggctacttcc
     2881 tgaggtattt cgtgggcaaa aaccgatatg gcaactacaa ttccaaaaga aggcgaatga
     2941 agaaaagcag cagcgataac aataccatcg agaaagttgt ggaatgaatc gccgatcata
     3001 agcattaaag ccccggcttt gctgtgtctg tcgcaatttt catcggcgca aatacgccag
     3061 agcattattt tctcgataat aaagaaaaat aaaataccag ccagcacagc ataagatatt
     3121 tgatacccgg caattttaat tgcattgggc aacatgccta aaaatgcagc ccctaacaaa
     3181 gttcctccgg caaaagcatt aatgtgtttt gttaatttct gcaaatattc atttttgaac
     3241 ttcaacataa gcgatgccat taaaacactg cctatattac ccaatagtac ggctaaaata
     3301 attaatgtta tcatgttgtt ccttcttttt caaatatctt atatgtaaat aaaacgtgcc
     3361 ccttgcacaa cccaaaagag atgtcaatat ccatctttat ttgtttatca tatcgtaaac
     3421 caaacctgca acatcaccaa ccacactccg acaaatgatt ttgtggtgct tacgggcttc
     3481 aacatcagct tcggtggcat gtttcttgag cagttcaaag caattgcttg ttccatatat
     3541 atcttccacc atcgagttta actctcgcac ttttttgtag gcatgcagtt tggtttccat
     3601 atcctttggc atttcattgc catatttcag gccaagcacc ataaatcccc cggttacagc
     3661 cccgcatttt tggcgtaagc gccccatacc gcctccaaaa gttgatgaaa ttagttttgc
     3721 ggttctttcg tccaactcaa actctttgct aaaagttaat aaaacagatt gagaacaggc
     3781 atatccttcg tcaaacaggc ccttggccat gtctatagct tctttccgtg tcattgtttt
     3841 agttattggt aattgcttgt aagcagcatt tttcaattgc cttcagaaca aaatccgggg
     3901 cagcacatgg tttccagttg tcgcgaccta caaaagcaag ttcggtctca gcctcattaa
     3961 ttagttcatt ttgctcgtta taaagcttgt aagtaaacca tatccgtacc ccttttatag
     4021 ccttgagggt agtttttacg gtaagttgct catcgtaatg agtagttttt aaaaatttga
     4081 aattcatact aataacgggt agcatgtagc ccgcctcttc aaccgaatgg tacgagatac
     4141 cgatacttcg gaacaattcc caacgggcag tttcgtaata tttagcgtaa ttggaatgat
     4201 gtaccgtgcc catttggtcg gtttcgggat acattaccct aaatgtatat acgtgttcta
     4261 tcattgtttt taatttttgg taatatttat caataactgg ccaaatgcag tttttatggc
     4321 tatatcatca aatagctcgt gaagggctcc attgtcacat acttcgcata gcttttcgtt
     4381 gataggaatt tgggccaaca ggggaatatt aaattctttg gacaacacat tacccccatc
     4441 ttttccaaaa agaaaatact tctcatcagg atgctgagaa ggggtaaacc aggccatatt
     4501 ttctactatg cccaatacag gagtaccaat aaacttatcg ttgtacatgt ccacagcttt
     4561 tttaacatca gccaatgcca tgttttgcgg agttgtaacc acaatagccc ctgatgtttc
     4621 aaaattttgc aaaagcgtga tatgtatatc gcccgtacca ggaggggtat caataataag
     4681 atagtccagc tcgccccatg atgtatctgt caacagctgg gtaataccgc tcgaaaccct
     4741 tggcccacgc cagatgacag cctgcttatt gtctataaaa aaaccaatag acatgagttt
     4801 aatgccaaat ttaacaaaag gctccatgta ttgtttgcca tttttttcga atactgaagg
     4861 cttctcgcct ttaagattaa ataaagttgg cactgatggc ccataaatat cagcatcaag
     4921 caagccggta gcatagcctt ccattgcaag cgacattgcc aggccggcag ctacagtgga
     4981 ttttccaacg cctcctttac cagaggccac aatgataata tttttaatgt tgggaagttt
     5041 tgtttttgca atctgattca tgataactga aaattttaaa aatgtgaata ttcaaggaaa
     5101 aagttttgcg ccgttggtgc atgtttcatt tagctcaata caatggacac gcgtattgcc
     5161 tttgtcgaag aattgttggg ctttttcgag tataatttcg gcaagatgtt cggacgtagg
     5221 gtttttactg gtaacaaaca agcgttgccc tgtatcgcgt atatactggc acatagggtc
     5281 atgctcccac aagatagtag catggtcaag cttttcgtca atccattgtt ttaacacctt
     5341 ggtatcttga aaatcaatga ccatatcttt attatcgagc gtttcggctt cgatagaaac
     5401 tcgcaccaac cacgaatgcc catgattatt ggtacatttg cccgtgtagt ttttgtttaa
     5461 ccgatgagcc gcttcaaagc taaactcttt ggaaatgatg tatttcatat tgagttaacg
     5521 gatatttatt aaatatttaa agaacagaaa accttttcgt aaattttgaa tatctctttg
     5581 cttatttctg attgaggagc atattcaata atacttttac aattaaccat tgcgtttacc
     5641 acattttcat caaaagaaag acggcccaca atagcaatat tttgctgaat gcaccatgct
     5701 tctatttgcc ccgaaatatt ggggttcagg tcgtacttat tgataaccac ataaggcttt
     5761 gccttaaagt tggccactat ttctaaaact cttttcatat catgaaaccc agacatagaa
     5821 ggttcggtta ccaccaccac cttgtttacg ccagttacag atgaaataac aggacaacct
     5881 gtacccggcg ggccatcaat gataattatg ttgctatcta tttcattagc cacatttctg
     5941 gcttgttcgc gcaccaggtt gactaattta cctgaatttt cttcgccagg ggctaaacgt
     6001 gcatgtacca tccggccatt tcggtaatta ccaatatacc atcggctttt atcacttggc
     6061 accatagtta tagcctgaac agggcaaatc cgagaacaca gtttacaacc atcacaagaa
     6121 gtatcagaaa tgaacacctt gccattgctc atgctgatgg catcaaatcg gcaataagcc
     6181 tcacattgcc cacaactggc acatatatca tagttgataa cagccctgta ccccgaaata
     6241 tatacttctt ctttatcatt gacaggctgc aatactaaat gcaggttggc tgcatcaaca
     6301 tcacagtctg ctacaacaat attattgcca attgtagcaa aagctgcact caggctggtt
     6361 ttaccggttc ctcctttacc gcttaatatg gctatctctg tcattgtatt tgttttttga
     6421 cgagttgata taaagcaatg aaacgctcct gatattctgg cttttcatca acaagcatct
     6481 ttccttcaga ataaatacgg gctatttctt tgtcaaaagg tatttccatg agcagagtaa
     6541 aactgttttg ctcgagataa ttatacacat cgctgttgcc cagtgttgaa cggttgacta
     6601 caacaccaaa aggttttcca atttgctgaa gggtttctat ggaaagcttc aaatcattaa
     6661 gcccaaatgg cgttggttcg gtaaccaata tcacataatc agctttatca acagtggcaa
     6721 taaacggaca agagatgccc ggaggcgaat caaagatatt cagcgtattg gcctgagctt
     6781 cttttattgc cctttttatc accggtactg gcgaataaac gcctacctca gcacaccctt
     6841 cgataagctg tgaatgacaa ctgagataaa cagaatctac cttgcccact gtttttggct
     6901 tttctgtaat agcgccatac agacaagcta ctgaacatgc tccgcaatca tggcataaat
     6961 cctctacaac ctgtatatat tttgaaggct gcagatataa tatagcattg taattgcagt
     7021 attcgtaaca ttttccacag aatgtacatt ttggggtatc aatcacgggg atattttgtg
     7081 ttaccacatt gctggcaaaa gtttccccct ggataaattc catcacatta ggctcctctg
     7141 catcacaatc tacaaggatt acagtcaccc ctctcttttg caaggcatga aagaggttgg
     7201 tagaaactaa cgtttttccc gtaccccctt tgccacttgc tacagctatg ttcatcgttc
     7261 taaactgttc ttttttacaa caaaatcacc tttgttaact tgcaaagcat ctgcaatgac
     7321 attgcatttg accataagca taaaatacac ttctttttgt gtcctgtgca aaaggccttc
     7381 gagattaccc tgtcctttgg agataacaat atcggctttt tcaaaaatat gcctaaattc
     7441 atctgagcaa tgttcaacaa tagttgaagg ggcgtcataa ccgttggaaa taacctttgc
     7501 caccttgtct atacctacat agcgtgcatc gtccagcgta acatcgttta tcaccggagc
     7561 ccctcgcaca gcaaaataaa catcaggatg gccgacagtt tcaataaaaa gtttgtcaaa
     7621 gactatttca ccgttgttat cgccaaggta aagaatggtt tgtgcaccgg caatggcctt
     7681 tttcagctca gccgaatggt caatagcaaa atgcgaataa agtactttgt ctatggtggc
     7741 atgcaggtca taatttttac tcacagcaaa atcaataata ttgccggcaa tggccaaacg
     7801 caatgcggta tcaaaagggt tgacagacgt ttgaactttt tctttcaact cattatacat
     7861 cgccagtacc aaatcattgc taagcttttt accctctctg tgcgggtcgg ggttattggt
     7921 atatttgtta agagctatgt gcaaatccct tgaaaaatac ggagctgaaa agcgctcaca
     7981 atgtttaaga tagagcgcgg ccatatcgcg ggtgaaatta tctttcgccg aagctgatag
     8041 cgtcccttct tcaagcaagc gctcataagc ctttgtaaaa caaaagaaac aacgataatc
     8101 tgaaatcatc tttagcgttt ttaatggtcg caaccgtgtt ggtgatcgtg gttgcagtag
     8161 ttggcagtca gactgagttt gttgtctata aattcgcgta caagctcatt aggagccttt
     8221 attggcgctc caataaacac gtttatattt tgctggtgaa aaaggtcgat ggctttttgc
     8281 cccatgcccc cgccaataac atctgttaca ccaaattcgg caacccaacg tggatataac
     8341 cctggcacat gctctggcgg ggtaacttct gtcacgctgg taatttgatt attttctaca
     8401 tcaacaattg caaattgttg acaatgacca aaatgggcac ttaaaacccc attttccatc
     8461 ggtatagcta tctttttctt cattttcttt tatttatagt gttttgttct ttttaaaaat
     8521 cctaaacttg tccaaaacgc ctgcccaacc ctctaccagg gcctttaccc ataccacggc
     8581 ctttacctct gccccaacct tgtccgaagg ttgtatcttg ttcccggttg ttcatgttac
     8641 ggttttgaaa aatttcttct tcagttttgc ctttattatc gggattgcac cgacccattt
     8701 ttctacctgt cataggccct tctccattgg ggcctcttct gtttaatcct ggcataatta
     8761 cctccttttt taactcccaa acccaatttg aaaagggttc agaaatgctg attattttaa
     8821 tccacttttc agcctttttc caaggccctg gcctcctccg gatttccggc gcaagcccat
     8881 gcccttgcct aacttatcaa gagcttcatc ttggttaata acccgacact ggccgagttt
     8941 tcgccctgtt tttggccctt tctgttcagg ccctttcccg tccaaatgtg ccatatttta
     9001 atttttaaaa tttactcgtc ttacgctttc tcactcttcc ttcccttttt ccaacaattc
     9061 aattatctct gcaattgttt ttttcgggtc atttactacc accaactgaa tttgcaggct
     9121 gtcgaaaata gatttaacct tagtaccaaa ctcacccgaa acaacctttt tcactcctcg
     9181 tgaagcgata agattgacag aagcagggcc agcgccttca acactctcta agttaggatt
     9241 agggataaac tctgttgcct gagttttggt gtcataaata acaaaataag aacaacggcc
     9301 aaaacggctg tctaaagttg attctaagct gttgccagtt gatgttattg ctaccttcat
     9361 attgtttatt gtttattgac aattattgtt tctcattctg tgatggtgtg gcgcaccctt
     9421 gcgcaccatc tgacatttac attcaggaca tatttctttt tggcatggaa tacccgaagt
     9481 atgttccttt tcttttccac attttggaca ggtgcaaata tcctcagtgt ttgcctgttc
     9541 ggggctatcg ctattttgct ctatattttt agaaccacaa agcgcacaag cattaacttc
     9601 tgtatcttta gcaggatggt tgaaatagca tccacagaaa tggcaataat accattcgct
     9661 gtcaaaatac actttgcccc cttcaaaaac aattactttt cctaaaacaa aagcctgtgc
     9721 tactttacgc cttgcacttt cataaatgcg ggcataagta ggccgcgaca catccattat
     9781 ttgagctgct tctacctgtc catacaattc aaagtcgctc agacgtatag cctcatactc
     9841 ctcataattc aatataacag gagtgctttc ttcactcagc ccgattggct tataccccct
     9901 gaaatgagga ggattggtca tttttcttat tcgttttgga cgtggcatag taatgagcat
     9961 ttgttcaata caaaagtaat gaacatttgg ttaataccaa ataattttgt atatttaaca
    10021 tatcaaactt aaaattgaaa aactatggaa gaaaaccaag ctatgtcgat gccccgcatc
    10081 ggagaatctg ccccagaatt taaggcagta acaacacagg gaaatattaa ttttcccgct
    10141 gatttcaaag gtagttgggt aatattgttc agccatccgg ccgattttac cccggtttgc
    10201 acctcagaat ttatgacttt tgccagcatg gaaagtaagt ttgccgaggt aaactgcaag
    10261 ttagtaggcc tctcggttga tgggctttac agccatattg catggttgcg tactattaaa
    10321 gaaaagattt cgtacaaagg catgaaagat gtcgaagtaa agtttccgtt gatagaagac
    10381 ataaccatgg aagtagccaa aaagtatggt atgatacaac ctggcgaaag taataccaaa
    10441 gctgtgcggg cagtattttt catcgacccc aaaggcatta tccgcacgat tatttattac
    10501 ccgctcagct taggccgcaa ctttgacgaa ctataccggg tagtagtagc attgcaggct
    10561 gccgacagct ttggtgtggc catgccagcc gattggcgac ctggcgatga cgttattgta
    10621 ccaacagcag gctcatgcgg tgtagctaaa gaacgcatgg atggcaaaga agaaggcgta
    10681 acttgtcatg actggttctt ctgtacaaaa aagatagctg aagacaaagt ttgggcggct
    10741 gtaaagaaga aataaaccaa acgtaaacaa acaaaaaaag cttaccagtc ctcatattga
    10801 ggaggtaagc ttttgtttt
//
LOCUS       DAOSPF010000090        10726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6139_length_10726_cov_7.895792, whole genome shotgun sequence.
ACCESSION   DAOSPF010000090 DAOSPF010000000
VERSION     DAOSPF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_10726_cov_7.895792"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1045)
                     /locus_tag="PK252_13065"
     CDS             complement(20..1045)
                     /locus_tag="PK252_13065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT15683.1"
                     /translation="MSKKNLRGLLAFLSVGSIMFLFSSCLDIEQQDMRSRDNDIAELQ
                     SYLSKKNITLGRTDTIYNIYLDTSKRLASAIAPAKNEYILADYNLRLVSTNRVVETNN
                     HVLASKVYISTDNTLPPVSTIAGPVKISLSGTFLGMYYAMKLMKEGDSAVWVIPSDLA
                     FSSYSTDFIPSYSSLVFEIKVLKVIKDPVAYDKAFWKSWIKDSLGKVSPVDTALANYA
                     DSTETGIYISKIINGDTSQTINLGQKVTLAYKGCLNDGRYFAGPIKYDTLTYYAGSSD
                     LIDGFEEAVMMLNKGARAKIFVPYYHAYGKNGKLNSIGQIVVPSYCSLYYEIEIVTSV
                     PLKSGRY"
     gene            complement(1081..2016)
                     /locus_tag="PK252_13070"
     CDS             complement(1081..2016)
                     /locus_tag="PK252_13070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT15684.1"
                     /translation="MSNVYTFNYNASFVIFMNRITFLFLAVCLFATLSCDNKYPGYKK
                     SSDGFYYKLLAFDDSSKKAEIGDYITIDISYKTIHDSLFFRGQRKLKLTKPAFEGSID
                     YCFAMLSKGDKAEFIINANNFFIYTLQKNLPTFLKNDENMKVDVEMLDVQSEIDYENQ
                     KKAFLKWVEDFGQYEKEILSQYIQQNKINIRPTSSGLYFISIKPGGKNRVNPGDTVIV
                     DYEGKFLDGKFFDSTKKRHESFQFVYGTEWQVIKGLEEAIGMMGEGEKAIVIVPSDMA
                     FGKDGSSTGIIPPFTSLIFEVELVKIIPGAQPKEI"
     gene            complement(2119..2436)
                     /locus_tag="PK252_13075"
     CDS             complement(2119..2436)
                     /locus_tag="PK252_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15685.1"
                     /translation="MGIGFSYASGMTMKNVKVEQNNNNKTKIESDVKYKPAQMIDLTI
                     CKSWGQRLRFNLEFGYSIKVSGGEYEIVKSEAKNVSDSYDKATKLMSPGGLILAVGLS
                     YGF"
     gene            complement(2403..2669)
                     /locus_tag="PK252_13080"
     CDS             complement(2403..2669)
                     /locus_tag="PK252_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15686.1"
                     /translation="MKRAFLLALIFISLSAFSQKRTYAGLGAGINNISGMAGLNFETP
                     IDTNFSVKIGVGAGWGIKLVSRPNITGVILHRWGWASVFRMLQE"
     gene            2897..3910
                     /locus_tag="PK252_13085"
     CDS             2897..3910
                     /locus_tag="PK252_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT15687.1"
                     /translation="MKTLSMNCWRKTVILFCILTLISCQKKSEHKIALMFPYTTCSRM
                     AIEEQAFKAKASALNCEAVFTDAQSDEALQRKQAKELIDQGVDVIVVMAVNAYTAAEI
                     VREAHAANVKVIGYDRLIHNCDLDFYISHNNYDVGKLMAEYALKVRPNGDYLVLGGDK
                     SDKNAIFVKTGQLETLQPAIKSGNIKIVFDVYVEDWSTDNAYINMSEYLKLNANNAPD
                     VILTSYDGLVYGARKALDEAGITKDIVITGQDAEPQALKNIMSGKQTMTIYKPLKALA
                     EKAVETAVKLANGEQPDTTGSINNSRKDVPSYLIAPVVVDKNNMLETVVKDGIISAAE
                     LGL"
     gene            complement(4136..7084)
                     /gene="dnaE"
                     /locus_tag="PK252_13090"
     CDS             complement(4136..7084)
                     /gene="dnaE"
                     /locus_tag="PK252_13090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT15688.1"
                     /translation="MYLNCHSYYSLRFGTMSVEELVNNAAQYGVKAMALTDINSTMGV
                     VDFYKACILSNIHPIVGIEFRNKGQLQFIALARNNDGFREINEFLTLYQVENNKAIPS
                     VAPDFNNVYIIYPLHDKLDRRLRENEFWGVRPSDLTQFPLMQHKIRRAKCVALFPVTF
                     NSVQDYELHLNLRAIDENTLITKINKAHLAGKHEAFLPIDEIQALFDDFPYLRENTER
                     LMQGCSIDFKLGQPKNKRFFTQSKYDDKVLLEKLAFEGMLYRYGKDNIEAYRRIRHEL
                     DIIDKLGFSAYFLMTWDIIQFSMSRGFYHVGRGSGANSIVAYCLKITNVDPIELDLYF
                     ERFINPKRSSPPDFDVDFSWKERDDVLDYIFRRYGAKHAALICAISTFRDRSIFRELG
                     KVHGLPKDEIDCLVTKTDNELNKNGLTARLFSIGNRLMDFPNLRSIHAGGVLISEEPI
                     TYYTALDLPPKGHLTTQWDMYVAEDLGFEKFDILSQRGIGHIKDCALIVKENRGIEID
                     VNNVASFKKDEKVRSRLKTGEALGCFYVESPAMRGLLKKLRCDDYRTLVAASSIIRPG
                     VAKSGMMKEYIRRFNNHSAIKYLHPLMEEHLHETFGVMVYQEDVIRICHHFAGLDLAD
                     ADVLRRAMSGKYRSRTEFAKVVEKFFANCRAKGYPDSLTNEVWRQIESFAGYSFSKAH
                     SASFAVESFQSLYLKTYFPIEHMVAVINNFGGFYQTWVYVHEAQRLGAAIHLPCVNRG
                     KTLTTVQGVDVYLGYGLVQNLQKEVIHSIEKEREEHGPFLSLADFIGRVPVSLEQLLI
                     LTRIGALRFTQKSKKQLLWEIHRLVKSSAKPVYKQAALFAPADRKFQLPAFEHNKLED
                     AYDEIELIGFPVSISSFDMLETRFRGEIMACQMISFVGKKVRMLGRLVAFKNVGTSDN
                     RLMQFGTFLDVQGNFFDTVHFPDSLQQYRFKGSGVYLLLGKIVEDFGFPSLEVEKMAK
                     MPYKKDPRYE"
     gene            7301..8254
                     /locus_tag="PK252_13095"
     CDS             7301..8254
                     /locus_tag="PK252_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15689.1"
                     /translation="MKRKIYISISLLTIILISIVVTAFQNKPQQLSAPLLLPQVVSAK
                     KSYVFTQALTSKFRNDFKIENAIFPDTTVILFEFIPVGGWLKKLSDSKLSLNYYATAY
                     NKGKLFIAGGRDISGKVVNTFYEYDLAGQQWINRPSMKTAREFFALESHDNFIYAIGG
                     TDKTEAEVYDMSSNQWTTKPLNYTGCSPMKEIICYENIDTLFFMFNNNGEMQILNIKN
                     MTVQKGPTPPFKMKNFDACTYNKKIYISAGYDESGINANVYCYSTADSLWSSIGKVGS
                     PRCLSGLVYFGSMILHIGGSFTIPSQKCRILSDTYIFRLPN"
     gene            complement(8251..9210)
                     /locus_tag="PK252_13100"
     CDS             complement(8251..9210)
                     /locus_tag="PK252_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOT15690.1"
                     /translation="MGKRFFLRYLFVFIVSFSFGFAWGQKYSRQDYIQKYSPIAVEEM
                     KRSGIPASITLAQGMVESGCGNSSLAREANNHFGIKCHKWQGKKYFQDDDAPNECFRK
                     YSNAEESYRDHTDFLMNTSRYAFLFEYKSDDYKGWAHGLKKAGYATSPSYARDLIRTI
                     EENELHRFDKGDYTYVPSVKNKPRMAASDENFVIDIKRRKIFTNNDVQYIVAEEGDNL
                     ISLTKEMDLFSWQLVKYNDLPADYHVTSGDRLYIQPKRWKAQFGNDFHIVAENETMHS
                     ISQLYGIKLKKLYYKNRMEIGSEPKIGQKIWLRKRMPKEVLQN"
     gene            complement(9222..9899)
                     /locus_tag="PK252_13105"
     CDS             complement(9222..9899)
                     /locus_tag="PK252_13105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT15691.1"
                     /translation="MEVLFEDNHLIAINKQTSDIVQPDKSNDISLEEKIKTYLKQKYQ
                     KPGDVFLGVVHRIDRPVSGVVLFARTSKALARLNQMFQDKSVKKIYWAIVKEQPPKEA
                     DTLIHYIVRDTRKNKSFAYDKEVKESKKAILHYKILDKSDNYYLLEIQLETGRHHQIR
                     CQLSKIGCPIRGDLKYGYNRSLDHGGINLHARKISFTHPVKNEPVEIVANVPEDKLWK
                     YFENRNK"
     gene            10035..10108
                     /locus_tag="PK252_13110"
     tRNA            10035..10108
                     /locus_tag="PK252_13110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10069..10071,aa:Met,seq:cat)
     gene            10236..10568
                     /locus_tag="PK252_13115"
     CDS             10236..10568
                     /locus_tag="PK252_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOT15692.1"
                     /translation="MDIKFVWVEKHRRHAFILTAIDTFTRVVLGWTVAFQIKQETVKQ
                     LWSRIIENHLQPHDCINKQMDIEVRNDNDSRFVARSVQQFFRDNHLGQVFTHPYTPQE
                     NGHIEDIP"
BASE COUNT         3337 a         2131 c         1867 g         3391 t
ORIGIN      
        1 agccaaattt gtttaatttt taataacgtc ccgattttaa tgggacacta gtgacaatct
       61 ctatttcata atataaagaa caatatgacg gcactacaat ttgaccaatg gaattcaatt
      121 ttccattttt tccataagca tggtagtatg gaacaaaaat cttggccctt gcccctttat
      181 taagcatcat tacagcttcc tcaaaaccat caatcaaatc actggagcct gcataatatg
      241 ttaatgtatc atattttata ggcccggcaa aataacgccc atcgttgagg caacctttat
      301 atgccaatgt tactttttgg cctaagttaa ttgtttgaga ggtgtctcca tttattattt
      361 tacttatata tatgcccgtt tctgtagaat ctgcataatt ggccaaagca gtatctactg
      421 gagatacttt tcccagactg tctttaatcc agcttttcca aaaagctttg tcataagcta
      481 caggatcttt aattactttc aatactttta tctcaaacac cagcgatgaa taacttggga
      541 tgaagtcagt agaataagaa ctaaaggcta aatccgaagg tattacccaa acagcgctgt
      601 ccccttcttt cataagtttc atggcataat acataccaag aaatgtacct gataatgaaa
      661 tttttacagg ccctgcaata gttgataccg gaggtaaagt attatctgtt gaaatgtaaa
      721 cttttgaagc caaaacatga ttgtttgttt ctactactct gttggtactg actaatctca
      781 ggttataatc tgcaagaata tattcatttt tagcaggagc aatagccgag gccaatcgtt
      841 ttgaggtatc caggtaaata ttataaattg tatctgttct tccaagtgta atgttttttt
      901 tgcttaaata tgactgaagt tcggcgatgt cgttatctct gcttctcata tcttgttgtt
      961 ctatatcaag acaagaagaa aataaaaaca tgatactacc aacacttaaa aatgctaata
     1021 accctcttaa attttttttg ctcataaccc aatttttaaa acatgtttac catagatacc
     1081 tcaaatttct tttggttgcg cccccggaat aatttttacc agttcaactt caaaaatcaa
     1141 tgatgtaaaa ggcgggataa tgccagtaga agaaccgtct ttgccaaatg ccatatctga
     1201 agggacaatg acgatggctt tttcgccttc gcccatcatc cctatggctt cttctaagcc
     1261 ttttattacc tgccactctg ttccatacac aaactgaaac gattcatgac gtttttttgt
     1321 tgaatcgaaa aatttcccat caaggaactt accttcataa tctactatta cagtatctcc
     1381 cgggtttacc ctattttttc ctccaggctt tatagagatg aaatacaaac cactggaagt
     1441 gggccgaata tttattttgt tttgttggat atattgcgag agtatttctt tctcatattg
     1501 tccaaaatct tcaacccact taagaaaagc ttttttttga ttttcataat ctatttcaga
     1561 ctgaacatca agcatctcta cgtccacttt catgttttca tcatttttca aaaatgttgg
     1621 gagatttttt tgaagggtat aaataaagaa attattggca ttgataataa attcggcttt
     1681 atctcctttt gaaagcatag caaaacaata gtctatagag ccttcaaatg cgggtttggt
     1741 cagctttagc ttccgttgcc ccctgaaaaa taaagagtca tgaatagttt tataggaaat
     1801 atcaatcgtt atatagtctc ctatttctgc tttttttgaa ctatcatcaa aagccaaaag
     1861 tttataataa aatccatccg agcttttttt gtagcccgga tatttattgt cgcatgacag
     1921 agttgcaaat aagcaaacag ccagaaataa aaaggttatt cggttcataa aaataacaaa
     1981 tgaagcattg taattaaatg tatatacgtt gctcaaaagt aataaaattt taaaccgata
     2041 tgcatttatg attccaatga aataaaaaag gcggaagttg aaaaatcaaa atccgcctta
     2101 acactttatc aattaatctt aaaaaccata tgaaagtcct actgctaaaa tcaaacctcc
     2161 cggagacatc agttttgttg ctttatcgta agaatctgaa acattttttg cttcagattt
     2221 tactatttca tattctccgc ctgatacttt tattgaatac ccaaattcaa ggttaaacct
     2281 taacctctgc ccccaagact tacaaattgt aaggtctatc atctgggctg gtttatattt
     2341 aacgtcagat tcaatttttg ttttgttatt attattttgc tccaccttaa catttttcat
     2401 ggtcattcct gaagcatacg aaaaaccgat gcccaacccc agcgatgtag gataactcct
     2461 gtaatatttg gccgagatac cagttttata ccccaaccag cacctactcc tattttaaca
     2521 gagaaattag tatcaatagg tgtttcgaaa ttgagccctg ccatacccga aatattgttt
     2581 atcccggcac ccaatcctgc ataagtcctt ttttgtgaaa atgctgacaa tgatataaaa
     2641 atcaatgcta ataaaaatgc tcgtttcatc cgaacaaaat tgaattttaa aggtcggatg
     2701 gaactcgcat gattttgttc atacgtgttt gtgtttactt ctaaacatgc tcgtttcatg
     2761 gtataaattt taaatgtttg ttaaactttt tcaatatgac atattactat ttgttttccc
     2821 ccaacatttg ccaattaaaa taattacaga tatatttttt tgctattttt gaggcacaac
     2881 ctaaaaaaga atttaaatga aaactttgag tatgaattgt tggcgtaaaa cagtaatcct
     2941 gttttgcata cttacattga tttcttgtca aaaaaagagt gaacacaaga tagctttgat
     3001 gttcccttat actacctgtt cgcgcatggc tattgaagaa caggccttta aagctaaagc
     3061 ctctgcgctt aactgcgagg ccgtttttac cgatgcacaa agtgacgaag cattgcagcg
     3121 taagcaagcc aaagaactta ttgaccaagg ggtagatgta atagtggtta tggctgttaa
     3181 tgcatataca gctgccgaaa ttgttagaga agctcatgct gccaatgtaa aagtcattgg
     3241 ttacgaccgc ctgatacaca attgcgattt agatttttac atttcgcaca acaattatga
     3301 tgttggtaaa ttaatggcag aatatgccct taaagttagg ccaaacggcg actatcttgt
     3361 tttgggcgga gataagagtg ataaaaatgc catatttgta aaaactggcc aacttgaaac
     3421 tttgcaacca gcaataaaat caggaaatat taaaattgtt tttgatgtat atgttgaaga
     3481 ctggtcaaca gacaatgcat atattaatat gagcgaatat ttgaaactca atgccaataa
     3541 tgccccggat gtaatactta cctcgtatga cggccttgta tatggcgccc gcaaagccct
     3601 tgacgaagct ggtattacta aagatattgt tattactggc caggatgcag agcctcaggc
     3661 attgaaaaat attatgtctg gaaaacaaac aatgacaatt tataaaccat tgaaggcact
     3721 tgccgaaaaa gctgttgaaa ctgccgttaa attagctaat ggagagcagc ctgatacaac
     3781 aggctctata aataattcga gaaaagatgt tccttcatat cttattgccc ctgttgttgt
     3841 tgataaaaac aacatgcttg aaacagtggt aaaagatggg attattagtg ccgccgaatt
     3901 aggactataa taaagattaa agagcgttga ttagaaactg gtcaccccgc tcagggctta
     3961 attataacga tgtttatttt actacaaaca ggacatcgct acgcgactaa ataatagcag
     4021 catagctgcg aactatttgt aggacaatca aatacatcaa atgtttctaa gccgcatagc
     4081 ggcgacctgt ttgtaccgga caacaatgat aaagaaaaat acaaggcctc aggcttcatt
     4141 catacctggg gtcttttttg tagggcattt tggccatttt ttcgacctca aggctgggaa
     4201 agccaaaatc ttccactatt ttacccaaca ataaataaac accgctacct ttaaaccggt
     4261 actgctgtaa tgaatcagga aaatgcaccg tatcaaaaaa attgccctga acatccagaa
     4321 atgtaccaaa ctgcattaat ctgttatctg aagtaccaac gtttttgaag gctacaagtc
     4381 gtcccaacat ccgtaccttt ttgcccacga aagatatcat ttgacaagcc ataatttcgc
     4441 cgcgaaaacg tgtttcgagc atatcaaaag atgatatact aaccggaaaa cctattagtt
     4501 ctatttcatc ataagcatct tcgagtttgt tatgctcaaa agcaggaagc tgaaactttc
     4561 tatctgcggg ggcaaacaag gctgcttgtt tataaaccgg cttagcactg ctttttacaa
     4621 gacggtgtat ctcccacaac aactgttttt tgctcttttg tgtaaagcgc aaagctccaa
     4681 ttctggtcaa aataagcaat tgctcaagac ttacaggcac ccggccaata aaatctgcta
     4741 aagataagaa tggcccatgc tcttctcttt ctttttcaat actatggatt acctcctttt
     4801 gcaaattctg caccagccca taacctaagt acacatcaac accttgaact gttgtaagtg
     4861 tttttccacg attaacacac ggcaaatgaa tagcagcccc cagcctttgt gcctcatgta
     4921 cataaaccca tgtttgatag aagcctccaa aattgttgat aacagccacc atgtgttcta
     4981 tgggaaagta cgttttgagg tataaactct gaaaactctc aacggcaaat gacgctgaat
     5041 gggcttttga aaaagaatac ccggcaaaag attcgatttg tcgccatacc tcattggtaa
     5101 gcgaatcggg atagcctttg gctctgcaat tggcaaaaaa cttctcgaca acttttgcaa
     5161 attccgttcg tgacctgtac ttaccgctca ttgcccttcg caacacgtcg gcatctgcca
     5221 ggtcaagacc ggcaaaatgg tgacaaatcc tgattacatc ttcctgatat accatcactc
     5281 caaaagtctc gtgcagatgt tcttccataa gcgggtgcaa atacttaatg gccgaatggt
     5341 tgttgaaacg gcggatgtat tctttcatca tgccgctttt ggccacaccg ggcctgatga
     5401 tgctactggc agctaccaaa gtacgataat catcacacct gagctttttg agcaagccac
     5461 gcatagccgg actttctaca taaaagcaac ccaaagcttc tcctgtcttc aagcgcgaac
     5521 gcactttttc atctttttta aaactggcta cattgtttac atcaatttct atgccccggt
     5581 tttcttttac tatcaaagca caatctttta tatgtccaat accacgttgg cttagtatat
     5641 caaacttttc gaaacccaaa tcttcggcta catacatatc ccattgggtt gtaagatgcc
     5701 cttttggagg caaatccaat gcggtatagt aagtaattgg ttcttcagaa atcaaaaccc
     5761 ctccggcatg aatgctgcgc aggttaggaa aatccatgag gcggttgcct atgctaaaaa
     5821 gccgggctgt cagcccattt ttattgagtt cgttatccgt ttttgttacc aaacagtcaa
     5881 tttcatcttt aggtaagccg tgtactttgc caagctccct gaaaatggaa cgatccctga
     5941 aagtagaaat agcacaaata agagctgcat gttttgctcc ataccgcctg aaaatataat
     6001 cgagtacgtc atcgcgttcc ttccacgaaa agtcaacatc aaaatcggga gggctggaac
     6061 gctttggatt gatgaaacgt tcgaaataca ggtcaagttc tatgggatca acgtttgtta
     6121 tcttcaaaca ataggccaca atactgtttg ccccgctacc acgccctaca tgatagaacc
     6181 cccgggacat tgaaaactgg ataatatccc aggtcattaa aaagtaggca gaaaacccca
     6241 acttatcgat gatatccaat tcatgcctga tacgacgata tgcttcaata ttatccttgc
     6301 cataccggta aagcatacct tcaaatgcca atttttcgag caaaaccttg tcgtcatatt
     6361 tactttgagt aaaaaatctt ttgtttttgg gctgtccaag tttgaaatca atgctgcatc
     6421 cttgcattaa gcgctctgta ttttctctga gataaggaaa gtcgtcaaat aatgcctgta
     6481 tttcatctat gggaagaaat gcctcatgtt tcccggctaa atgggcttta ttaattttgg
     6541 taatcaacgt attttcgtca atagccctga gattgagatg caattcgtaa tcctgcacag
     6601 aattgaaggt cacagggaaa agagctacgc actttgccct tcttatctta tgttgcatca
     6661 acggaaactg tgtaagatca gatggacgga caccccaaaa ttcattttcg cgcaggcgcc
     6721 tatctaattt atcatgtaaa ggataaataa tataaacatt attaaaatca ggggctacag
     6781 acggaatagc tttattattt tcgacttggt aaagggtaag aaactcattt atttcgcgaa
     6841 aaccatcatt gttgcgagcc agggcaataa actgcaattg tcctttattt ctgaactcta
     6901 taccaacaat aggatgtatg ttgctcaaaa tacaagcctt gtagaaatca acaacaccca
     6961 ttgtactgtt aatatcagtc aaagccatag cctttacccc atattgtgca gcattgttaa
     7021 ccaattcttc taccgacatg gtcccaaacc tcaggctata ataactatga caatttaaat
     7081 acatttttat ttaaaatatt gattcaatga tttttaacta aaatatactg gttaatattt
     7141 aatcaattat attgtttaaa atataagcca atgtaaaggt aaatccaaag aatatttgac
     7201 gcttcattat atttttttga tttatatttt ttaaaaattt gatttaaaaa aataattcat
     7261 taattttaaa ggtgttagca aaattcaaaa agacaatgcg atgaaaagaa aaatatacat
     7321 ttcaataagc ctgttgacaa tcatccttat ttcaattgtt gttacagcat ttcagaacaa
     7381 gccccaacaa ctttctgcac ctttactttt gccccaggtg gttagtgcca aaaaaagtta
     7441 tgtatttact caggccttaa cttcaaagtt tcgtaatgat tttaaaatag aaaacgctat
     7501 atttcctgat actacagtta ttttattcga gtttatcccg gtgggaggct ggttgaaaaa
     7561 attatctgat tcaaagcttt cgttaaatta ttatgcaacg gcatataata aaggaaagct
     7621 ttttatagcc gggggaaggg atatttcggg caaagtagtc aatacttttt atgaatatga
     7681 ccttgccgga caacaatgga taaacaggcc ttcaatgaaa acagcccgcg aattctttgc
     7741 ccttgaaagc catgataact ttatttatgc tattggaggt actgataaaa ctgaggctga
     7801 agtatatgat atgtcttcaa atcaatggac aacaaaacct ctaaattata caggatgttc
     7861 acccatgaaa gaaattatct gttatgaaaa tattgacact ttgtttttca tgtttaataa
     7921 caatggggag atgcaaattt taaatattaa aaacatgaca gtacaaaaag ggccaacgcc
     7981 accgtttaaa atgaaaaatt ttgatgcctg cacgtacaac aaaaaaatat atatcagtgc
     8041 cggatatgat gaaagtggca tcaacgccaa tgtttattgt tacagcacag cagacagctt
     8101 gtggtcttca atagggaagg tcgggagtcc gcgctgtttg tccggtttgg tttattttgg
     8161 cagcatgata ctgcacattg gcggctcgtt tacaatacca tcgcaaaaat gccggatatt
     8221 aagcgatact tatattttcc ggttacctaa ttagttttgt aaaacttctt ttggcattcg
     8281 tttacggagc catattttct ggccaatttt gggctctgag cctatttcca tgcggttttt
     8341 atagtacagt ttttttagtt taattccata taactggctt atgctgtgca tggtttcatt
     8401 ttcggctaca atatgaaaat cattaccaaa ttgagctttc cagcgcttgg gctgaatata
     8461 caacctgtcg cccgaagtga catggtaatc ggccggcaaa tcgttgtatt tgaccaattg
     8521 ccacgaaaac aagtccattt cttttgtcag cgagataagg ttatcgcctt cttctgcaac
     8581 aatgtattgt acatcattat tagtaaatat tttccggcgc ttgatatcaa taacaaaatt
     8641 ttcatcagag gctgccattc tgggtttatt tttaaccgat ggcacataag tatagtcgcc
     8701 tttgtcaaac cggtggagct cgttttcttc tatagtcctg ataaggtctc tggcataact
     8761 tgggcttgta gcatagcctg ctttctttaa tccgtgggcc caccctttgt aatcatcaga
     8821 tttatattca aaaagaaaag cataacgtga agtattcatc aaaaaatcag tatgatcacg
     8881 gtacgattct tctgcattac tatattttcg aaaacattca ttaggggcat catcatcctg
     8941 gaaatatttt ttcccttgcc acttatggca tttgatgccg aagtgattat tggcttctcg
     9001 cgctaaagag ctatttccgc aaccagattc gaccatccct tgtgcaagtg ttatacttgc
     9061 cgggataccg ctgcgtttca tctcttcaac agctataggg ctatatttct ggatataatc
     9121 ctgcctggaa tacttctgcc cccaagcaaa accaaaagaa aaagaaacta taaagacaaa
     9181 taagtatcgt agaaaaaatc tcttgcccat atctcaatca atcatttatt tctattttca
     9241 aaatacttcc aaagtttatc ctcaggtaca ttggccacaa tttcgacagg ctcgttttta
     9301 acagggtgtg taaagctaat ctttcgggca tgaagattga tacctccatg atcaagactg
     9361 cggttgtaac catatttcaa atcaccacga atagggcaac caattttact gagctgacaa
     9421 cgtatttggt gatgccggcc tgtctctaac tgtatttcaa gtaaataata attatcagat
     9481 ttatcaagaa ttttgtagtg cagaattgct ttttttgatt cttttacctc tttatcatag
     9541 gcaaaagact tgtttttgcg tgtatcgcgg acaatataat ggattaatgt atcagcttct
     9601 tttgggggct gctcttttac tattgcccaa taaatctttt tcacggattt atcctgaaac
     9661 atttggttca aacgggcaag ggctttgctt gtccttgcaa acaagactac accacttacc
     9721 ggcctgtcta ttcggtgtac tacgccgaga aatacatctc ctggtttttg atatttctgt
     9781 tttaaataag tttttatttt ttcttctaaa gaaatgtcgt tagacttgtc aggctggaca
     9841 atatccgagg tttgtttatt gatggcaatc aggtggttat cttcaaacag cacttccata
     9901 ctcaaaataa tactttctgc aaaagaccta aaaaagcagg agatttaaaa atttttttat
     9961 ccacatggta aaaatgcttt gttcatattt tgagaaaagt atatatttgt agcctcaaaa
    10021 caatgggcaa tgatggcgcg gtagctcagc tggttagagc gcatgattca taatcatgag
    10081 gtccccagtt caatcctggg ccgcgctact tgaaaaaaac aagccgctta cagagttttt
    10141 ctgtgagtgg cttgtttcat taaaaaaagt gctggagatc cttctggaga tctttttttt
    10201 taatttttct aattagtgaa atgaataact ggtaaatgga cataaaattt gtatgggttg
    10261 agaaacatcg ccgccacgcg tttattctta cagccataga cacatttaca agggttgtat
    10321 tgggctggac ggttgctttt cagataaaac aggaaacggt aaaacaactt tggtcgcgaa
    10381 tcattgaaaa ccatctgcag ccccacgatt gcataaacaa gcagatggac atagaagtcc
    10441 gtaacgataa cgacagccgt tttgtagccc gctcggtaca gcaatttttt cgtgacaacc
    10501 acctggggca ggtatttacc catccataca cccctcagga aaacgggcat atcgaagata
    10561 ttccataaca tctttttaca attgcccctc cataaacatt aggatattct ttataaagcg
    10621 aaccgtaatt tggccagtca aatacaaaaa cttcgccttg ttttggttgc cccgagagta
    10681 taaaatattt tgtgtaaaca caataatttg atttttaatt tatttt
//
LOCUS       DAOSPF010000091        10667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6199_length_10667_cov_4.829250, whole genome shotgun sequence.
ACCESSION   DAOSPF010000091 DAOSPF010000000
VERSION     DAOSPF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6199_length_10667_cov_4.829250"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK252_13120"
     CDS             <1..545
                     /locus_tag="PK252_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT15693.1"
                     /translation="KYFGKNHWRALTVISLGIGQGEILLTPLQLANFAAVIANRGYYY
                     TPHIVKAIGKETNYTKKYLKKNSATVAPHYFDYIVEAMYAVVEGGTAHNVRMDSIKVC
                     GKTGTAQNAGKNHSIFIAFAPKDNPQIAIAAVVENSGYGATWAAPVVSLMIEKYLTRK
                     VKRPELEKEIMESKLIDKNQ"
     gene            542..1804
                     /gene="rodA"
                     /locus_tag="PK252_13125"
     CDS             542..1804
                     /gene="rodA"
                     /locus_tag="PK252_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT15694.1"
                     /translation="MSTSRNTKANIDWVLIVLYVFFCLVGLLNIYSAGYTEEHKVFFD
                     FTQEHGKQMIWLAAALVVALCVMVIDARFFSTFAYIIYGMAILLLIVTLFAGKEIHGS
                     RSWIVISESVRFQPLELAKFATNLALAKFLSRIDIRIQDLSTRLNAFALLAVPVIIIL
                     LQNETGSMLVFAAFIFVLYREGLSGNVLIIGFATIFLFIFSLLINKFIVSGFILAVAV
                     LLFFFIRRSRKNIMNYSLIVAGVIAFVFSVNLIYEHALQPHQQKRIDVLLGKISDVKD
                     AGYNVNQSKIAIGSGGFFGKGFLHGTQTKFKFVPEQNTDFIFCTIGEEWGFVGSILVL
                     GMFVFFIIRILFVAERQRSAYSRIYGYGLASIIFFHVLINIGMTIGLAPVVGIPLPFI
                     SYGGSSLLAFTLLLFVFIKQDASRMQVL"
     gene            2089..3042
                     /locus_tag="PK252_13130"
     CDS             2089..3042
                     /locus_tag="PK252_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOT15695.1"
                     /translation="MKNVKYFAAVIILSFLVYSCKKVTTPSLTTNEVTCVTLKTATCG
                     GNITDDGGAAITARGVCWSTNQTPTVEENNTTDGAETGSYTSDISGLSENTTYYVRAY
                     ATNSEGTSYGETVSFMTQNYGPTVTDIDGNVYHTVHIGTQIWMMENLNVTHYRNGDQI
                     PNVINNSAWSVLTTGARCYWNNDSASAAPVYGALYNWYAVDDNRNLCPIGWHIPTDSE
                     WTVLFDYLGGETIAGFKLKSTSWIPGSGNNSSGFTAIAGGLRYNNGDYDGAGIHGLWW
                     TATEYNAQSARYCYLTSLNSGAHSSATTKLAGHSVRCVKDF"
     gene            3642..6434
                     /locus_tag="PK252_13135"
     CDS             3642..6434
                     /locus_tag="PK252_13135"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOT15696.1"
                     /translation="MRQTKITLSQLEDFLLKACDILRGKMDASEFKEFIFGILFLKRL
                     SDEFEVATAQVKKKFKHLSLEQLQELLDDPITYKPYTNFYVPPRARWNNSWIEKTEDD
                     KGEVKEIYRPALRDAKTGVGSLLNKALHAVEDANADLLSNVLKGIDFNIKKGKSSLPD
                     QRWIDLINHFNSELPALVNDNFEFPDLLGAAYEYLIKYFADSAGKKGGEFYTPNQVVR
                     LLVQLLKPKEGMEIYDPTVGSGGMLIQSLQYVEEQGQNGRNLMLYGQESNPTTWIICR
                     MNMILHNITSSTIEDGDTLEDPKNLKDGSWQKFDMVIANPPFSQNYSKANMKFQNRFA
                     YGFAPETGKKADLMFVQHMIASLNSKGRMATIMPHGVLFRGSAEKVIRKGIVDANLIE
                     AIISLPPALFYGTGIPACVLVINKNKDEKLKNKIFFINADAEYGEGKVQNFLRPEDIE
                     KIDYVFTHKIEIPKYSKLVDNKTIADEHDYNLNIRRYVDNTPEPEPEDVKAHLTGGVP
                     TIEIESQKLQYDKFKFQATEIFKAYKENYQEFKETITDKSLIKTNAEENKNVTATIAD
                     MQQAVDNWWLQARDEFSKIAAQPKHADKKSDEVNDPMAAYLTLGGTKMPKVRQELISS
                     LKTVLISKKVLDEFQVAGVFVNWWSNIKYDLKTIATIGWVPSLVPKEYFIETYFKAEQ
                     TAIQEAENLIGEKESALQEAIEAIEYEPEETEEGKEPEEATAKVIKDYLSAQIKDLKT
                     QAGAEKELKELTDTLEGIKTIEKEIKEAKDKVKELQADLERKIEYKMYGVDDAKEDIK
                     NLLAHTQKRIEELETQGEPEAKKEKAAYTKTLKALQADSLKMQEKLKGLDAFLESIGG
                     IITPEECKTLILQKHNSLVQQELLKYLNAEKRTLIAGIEKLWDKYKVTSQNLENKRKD
                     SLTKLNQFLTDLKYLS"
     gene            6434..7648
                     /locus_tag="PK252_13140"
     CDS             6434..7648
                     /locus_tag="PK252_13140"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOT15697.1"
                     /translation="MGEWKDITLGAVLKYEQPYKYTVESTQYNNSGVPVLTAGKSFIL
                     GYTSETNNLYSNLPVIIFDDFTSDCKFVDFPFKVKSSAMKFLKERNEEEISLKYFYEI
                     LQTLKLDSVGGDHKRRWISEYSKIKIKAPDFIEQTHIAEILSTADEAIAHTEALIAKY
                     QRIKTGLMQDLLTKGIDDKGNIRSKSTHKFVVKNGIEVPKEWEVENLGALCKKGCGSI
                     QTGPFGSQLHAEDYKEEGVPIITVEHLVDNRIAHENLPLVGEADYKRLKKYILNTGDL
                     VFSRVGAIDRCSITTESEDGWMFSGRCLRVRPGKLFNSHFLMYQLNFDLVRNYILSNA
                     VGSTMKCLNTTILSETPVFLPKTTEQIQISEKIESFENQLKQLNNRKAKLQSLKKGLM
                     RDLLSGKVRVKI"
     gene            7658..9637
                     /locus_tag="PK252_13145"
     CDS             7658..9637
                     /locus_tag="PK252_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15698.1"
                     /translation="MKSQNILSAYCFLAALTENNNDLYNHVYVPMCKRALSEYSRKGK
                     DYGTHLDIKETILRMYGIDVPESIVCKLIRGCEASLSRRKKEEINFKVLENGRTFQLE
                     AWVFKDFEEKFDKSQREANAIESLFQEYVINKRNFTEEIPSFADYLSRYKTQLSSFFE
                     GEFKSIERTEEETFFYHVEFLEELQRSNHTFFRIAQNLYLGAIVTGFLESGFDLDPRK
                     PSNECFFLDTPVLLRALDLQRAEETNPILELISLINKTGSTPKVLSITVEEIQKVINS
                     AIETYNNKISVTTINDACLRRKKDKAWLMTFSTNLQKNITSILQLEIENVGVDFIKEN
                     EKCADIPALQELRRCKNNAAHDVFAYLYVRKLRQTSVSIIQNAKVWFVTTNPSLLAFN
                     SDMRLANSVPEIVLPDMLTSLLWLKDPSKLVEEVKKIGLKELMTSTILEEVASKELIH
                     EYNLQIKKIEGIDSDSYNILLEAVAHYSANSIEKFIELAEQDQIKAREKALQIVEDER
                     TRKAEIQQKIADALTAEEREKSEKEIFAKKLEEFENQLRESNEAAKKEIDKLNEKVTS
                     HEDIISRQEKAIDEFGNKIDEQDEQISNLKKQIKKDTKKLLIGVIGVIIFVLTFIFKD
                     NLGNWTWFTAVLSGSGWIWGFGSFCINMIKILKKN"
     gene            9641..>10667
                     /locus_tag="PK252_13150"
     CDS             9641..>10667
                     /locus_tag="PK252_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15699.1"
                     /translation="MAKKEYKIGNKYSTRELIELAIEESHKSIPEHTDKTDPLVGAII
                     ATADGELLATAHRGELREGEHCEYTLIERKLVNMNLKGCVLYVTLEPCTDESRKKTKR
                     GCSTHIGKARLSKVYIGVEDPNPKIAGDGIKAIRNKGIGVEMFPSDLQELIWKDNAKF
                     KKEKELEALQAKLDEAKPQLSILEQTVVGSGITSFSDKAINQFVKESKAEFNYPSSDF
                     NEWAQGLGFIEKDAKDDGFLPTGLGIMLFGKTPENAFPQTIFKVEINYGTGKPEIKDF
                     KGPLVSQLPEIIDYVKDKGLKMTMEKSSGKREEIANFPFEVLLEAIANAVIHRDYSIE
                     GATNYLYI"
BASE COUNT         3680 a         1823 c         2196 g         2968 t
ORIGIN      
        1 ataaatattt tggaaaaaac cattggcgcg cgctgacagt tatttcgctg ggtatcggcc
       61 agggagaaat actgcttacg cctctgcagc ttgccaattt tgccgccgtc attgccaaca
      121 gaggctatta ttatactcca cacattgtga aagccatagg caaagagact aattatacca
      181 aaaaatacct gaaaaaaaat tcggccaccg tggctcctca ctattttgat tacattgtgg
      241 aagccatgta tgccgtggtt gaaggcggta cggcacataa tgtgaggatg gacagcatca
      301 aggtatgcgg caagacaggc actgcccaga atgccggcaa gaatcactct atttttatcg
      361 cgtttgcccc caaagacaat ccgcagattg ccatagctgc cgtggttgaa aactcgggat
      421 atggcgctac ctgggccgcg ccggtggtaa gcctgatgat tgaaaaatat cttacccgca
      481 aagtaaaacg gcccgagctg gaaaaagaaa tcatggagtc gaaattaatt gataaaaacc
      541 aatgagcacc tcgcgaaata ccaaggccaa tattgactgg gtgctgatag tgctgtatgt
      601 gtttttttgc ctggtgggcc tgctgaatat ctattcggct ggctataccg aggagcacaa
      661 ggtttttttt gattttacgc aggaacacgg taaacaaatg atatggttgg cggcagcact
      721 ggtggtggcc ttatgcgtga tggtgattga tgcgcggttt ttttccactt ttgcttatat
      781 catttatggg atggccatcc tcttgctcat tgtaactctg tttgccggca aggagataca
      841 cggctcccgc tcgtggattg tcatcagcga atcggtgagg tttcagccac tggaactggc
      901 aaagtttgcc accaacctgg cgctggcaaa atttctgagc cgcatagata tcaggataca
      961 ggacctgtcg acgcgactga atgcctttgc attactggca gttccggtaa tcatcatttt
     1021 gttgcaaaac gaaaccgggt cgatgctggt ttttgcggca tttatttttg ttctttaccg
     1081 cgaaggattg tcgggcaacg tactgataat aggttttgcc acaatatttt tgtttatctt
     1141 ttctttgttg atcaataagt ttattgtcag tggttttatt ttggctgttg ccgtgttgtt
     1201 gttctttttt atcagaagga gccggaaaaa tatcatgaat tattccttga ttgtggccgg
     1261 tgtcatagcc tttgttttta gcgtgaatct tatttatgag catgcgctgc agccgcacca
     1321 gcaaaaacgt attgatgtgt tactggggaa aatttccgac gtaaaagacg ccggttataa
     1381 tgtcaatcag tcgaagattg ccatagggtc gggtggtttt tttggaaaag gctttttgca
     1441 tggcacacaa accaaattta aatttgttcc ggagcagaac accgatttta ttttttgcac
     1501 cataggggag gaatggggct ttgtgggcag cattctggtt ttggggatgt ttgtattttt
     1561 tatcatccgg atactttttg ttgccgagcg gcaacgttcg gcctatagcc gcatttatgg
     1621 ctacggcctg gcttcgataa tattttttca tgtgctgatc aatattggca tgaccatagg
     1681 gttggctccc gttgtaggta tacctttgcc ttttataagc tatggcggtt cttcgctgct
     1741 ggcttttacc ctgctgttgt ttgtgtttat aaaacaggat gccagcagga tgcaggtgtt
     1801 gtaaaacaag gtgttttagg gttatcggat atttcttgaa aaaaagttaa aatatcttta
     1861 cgctaattaa ttatttatat atttgtgggt aacattatat gagctttata gttgatcgca
     1921 aatgtttaca tgcgtttaca aatggattta ataaaaattt aaaacactga cctttaaata
     1981 attataatta taaaaacaga ttgctgctac gacaaaacta tagtaaaaat aacagtaata
     2041 ggttagcaga agcgttagcg ttcattggca aaacaaaaat tctctattat gaaaaatgta
     2101 aaatactttg ccgctgttat tattttatca ttcttggttt atagttgcaa aaaagttaca
     2161 actcccagtt tgacaacaaa tgaagtcacg tgtgtgacac taaaaactgc tacttgtgga
     2221 ggaaatatta ctgatgacgg aggtgcggca attaccgcaa gaggggtatg ttggagtact
     2281 aatcaaacac caacagttga ggaaaataat actacagatg gtgcagaaac tggtagttat
     2341 acaagtgata tttccggatt atcagaaaat actacttatt atgttcgcgc atatgcgaca
     2401 aatagtgagg gaacaagtta tggagaaact gtttcattta tgactcaaaa ctatggacca
     2461 acagtaactg acattgatgg taatgtttat catacagtac atattggcac acagatatgg
     2521 atgatggaga atttaaatgt aacacattat cgtaatggag atcaaattcc taatgtaata
     2581 aataattcag catggagcgt tcttacaaca ggtgcaagat gttattggaa taatgactct
     2641 gcttcagctg ccccggttta tggtgcttta tataattggt atgcggttga tgataacaga
     2701 aatctttgtc ccataggttg gcatattcca actgattccg aatggacagt actttttgat
     2761 tatttgggtg gcgaaacaat tgccggtttc aaattaaaaa gtacttcatg gataccaggc
     2821 tctggtaata acagcagtgg cttcacagcg atagccggtg ggttacgata caataatggg
     2881 gattacgatg gcgcgggtat acatggttta tggtggacag caacagagta taatgcgcaa
     2941 agtgcaaggt actgttactt gacttctctt aactcaggag cgcacagttc tgcaaccacc
     3001 aagcttgcag gccatagtgt caggtgtgtc aaggatttct aagctatttg attattacag
     3061 agacgcaatc gatttttttt atttaaatac aaattacaat gcgaaaaaaa agagtggata
     3121 accaacgaaa cgctaacaca tagagcgata gccgaaaagc ccttgccaaa aaagcccgaa
     3181 aagaggaaca ctctttcggg cttttcggtc tattgcctgt tcggcgaccc cggcattact
     3241 gttttaaacg taggggataa agtagctggt ttttcaatgg ttgtgtcagc gatgaagatt
     3301 ttttatttcc tccaaaaata tacaataaat aaatttttcc tctaacatta atttatatat
     3361 ttgtcggaga caataaataa acctaaaatg ctaaaaaagg agtcgggtgt aagtgtaaaa
     3421 agataacgac aaagttgaca atttgctatt taaagaaaaa ggaaaaagaa atattgaaca
     3481 cccaacgcac aaaacagctc atttgcaggc tgtataattt gtcccgattt atcgggaagg
     3541 cgataatcgc aattaaagct tgctaaagag atgcaatttt gcttacgcac ccgtgctaaa
     3601 atgctgcttt tgagatttaa ttgaaaattg aatgtataag aatgagacaa actaaaataa
     3661 cactatcgca acttgaagat ttcttattaa aagcttgtga tattttgcgt ggaaaaatgg
     3721 acgcttcgga gtttaaggag ttcattttcg gtatactatt tttaaaacgt ttatccgacg
     3781 aattcgaggt ggcaacagcc caagttaaaa aaaaatttaa gcacttatca cttgaacaat
     3841 tacaagaatt attggatgat ccaataacct ataaaccata cactaatttc tacgttcccc
     3901 ctcgtgcccg ttggaacaac agttggattg aaaaaactga agatgataaa ggtgaagtta
     3961 aagaaattta ccgaccagcg ttgcgtgatg caaaaaccgg agttggtagt ttgttaaata
     4021 aagctttaca tgcggtagaa gatgccaatg cggatttatt gagcaacgta cttaaaggaa
     4081 ttgacttcaa tattaaaaaa ggtaaatcat cgctgcccga ccagcgttgg attgatttga
     4141 taaatcactt caacagcgaa ttgcctgcac ttgtaaacga taattttgag ttccccgatt
     4201 tgttgggtgc tgcttatgaa tacctgatta agtattttgc tgatagtgcc ggaaaaaagg
     4261 gtggagaatt ttacacccca aatcaggtgg tgcgcttact tgtacaattg ttgaagccaa
     4321 aagaaggaat ggaaatttat gacccgactg ttgggtccgg tggtatgctc attcaaagtt
     4381 tgcagtacgt tgaagaacaa ggacagaatg gccgaaacct gatgttatac ggacaagaga
     4441 gtaatcctac cacatggatt atttgccgga tgaacatgat tttgcataac attacttcat
     4501 caaccattga agacggtgac acgctcgaag accctaaaaa cctgaaagat ggttcgtggc
     4561 aaaagtttga tatggtaatt gccaatcctc cgtttagcca aaattacagc aaggcaaaca
     4621 tgaaatttca aaaccgtttt gcgtatggtt ttgctcccga aacaggtaaa aaggcggatt
     4681 taatgtttgt gcaacacatg attgccagtc ttaattcaaa aggacgaatg gcaaccatta
     4741 tgccccacgg tgtattgttc cgaggtagtg ccgaaaaagt aattcgtaaa ggaattgttg
     4801 atgccaattt gattgaagcc attatcagtt taccccctgc attattttat ggtactggta
     4861 ttccggcttg cgtattggta atcaacaaaa acaaagacga aaaactcaaa aacaaaatct
     4921 tctttatcaa tgccgatgcc gagtatggcg agggcaaagt gcaaaacttc ttacgccccg
     4981 aggacattga aaagattgat tatgtgttta cccacaaaat agaaataccc aaatacagca
     5041 agctggttga caataaaacc attgccgacg aacacgatta caacctgaac attcgccgtt
     5101 atgtggacaa tactcccgaa cctgaacccg aagatgtaaa agcccattta actggtggag
     5161 tgccaaccat agaaatagaa agccaaaaat tgcaatacga taaatttaaa tttcaggcaa
     5221 ccgaaatctt taaagcctac aaagaaaact atcaggagtt taaggaaact attactgata
     5281 aatcgcttat taaaaccaac gctgaagaaa acaaaaacgt tacggctacc attgccgata
     5341 tgcagcaagc cgttgacaat tggtggttgc aagcccgtga tgagttctcg aaaattgcag
     5401 cacaaccaaa acatgctgat aaaaagagtg acgaagtaaa cgaccccatg gcagcctatc
     5461 ttactttagg cggtacaaaa atgcctaagg ttaggcagga gcttatttct tctttgaaaa
     5521 cagttttaat ttctaaaaag gtacttgacg aatttcaagt tgctggagtt ttcgtaaact
     5581 ggtggagcaa tataaaatac gacctgaaaa ccattgccac cattggctgg gtgccaagcc
     5641 tggtgcctaa agaatatttt attgaaacct atttcaaagc cgaacaaaca gccatacagg
     5701 aagctgaaaa cttgattgga gaaaaagaaa gtgccttaca ggaagccatt gaagcgatag
     5761 agtatgaacc cgaggaaacc gaagaaggca aagaacccga agaagcaacc gctaaagtaa
     5821 taaaagacta tttgtcagca caaataaagg atttaaaaac acaggcaggt gctgaaaaag
     5881 aactgaaaga gctaaccgat accctcgaag gcattaaaac cattgaaaag gaaataaaag
     5941 aagccaaaga caaggtaaaa gaactgcaag ccgatttaga gcgcaaaata gaatacaaaa
     6001 tgtacggtgt ggacgatgcc aaagaggata taaaaaacct attggctcat acacaaaaac
     6061 gaattgaaga actggaaaca caaggtgaac ccgaagccaa aaaagaaaag gcggcttata
     6121 ccaaaacctt aaaagccttg caagccgaca gcctgaaaat gcaagaaaag ctaaaaggtt
     6181 tagacgcatt cctcgaaagc attggcggca taattacccc cgaagagtgc aaaaccctta
     6241 tactgcaaaa acacaacagc ttggttcaac aggaattgct caaatacctg aatgccgaaa
     6301 aacggacttt aattgccggc attgaaaagc tttgggacaa atacaaagta acttctcaga
     6361 acttagagaa caagcgtaaa gactcattaa ccaaattaaa ccagtttcta accgatttaa
     6421 agtatttgag ctaatgggtg agtggaaaga tattacatta ggtgcagttc taaagtatga
     6481 gcaaccatat aaatatactg tagaatcaac acagtataat aattctggtg tacctgtcct
     6541 aacagcagga aagtcattta ttttaggtta tacaagtgag acgaataatt tgtattctaa
     6601 ccttcctgtt ataatctttg atgattttac ttctgattgc aagtttgtag attttccatt
     6661 taaggtaaaa tcttcggcaa tgaaatttct caaagaaaga aatgaagaag aaataagcct
     6721 aaaatacttt tatgaaattc ttcaaaccct gaaattagat tcagttggtg gtgaccataa
     6781 aagacgttgg atttcagaat acagcaaaat aaaaattaaa gcacctgatt ttatcgaaca
     6841 aactcacata gccgaaatcc tcagcacagc cgacgaagcc atagcccaca ccgaagccct
     6901 cattgccaaa taccaacgca ttaaaaccgg tcttatgcaa gaccttctca ccaaaggcat
     6961 tgacgataag ggcaacattc gcagcaaatc aacccataag tttgtggtta aaaatggtat
     7021 tgaagtgcca aaggaatggg aggtggagaa tcttggagct ttatgtaaaa aaggttgtgg
     7081 ttcaatacaa acaggaccat ttgggagcca acttcatgca gaagattata aagaggaagg
     7141 cgtgccaata atcactgtag aacatttagt tgataataga attgctcatg agaatttacc
     7201 attagttgga gaagctgatt ataaacgatt aaaaaaatac attcttaaca caggagattt
     7261 agtttttagt cgtgtaggag caatcgatag atgttcaatt actaccgaaa gtgaagatgg
     7321 atggatgttt tcaggtcgtt gcttacgagt aagaccaggt aaattgttta attcacattt
     7381 tttaatgtat cagctaaact ttgatttagt aagaaattac atactttcaa acgcagttgg
     7441 ttctacaatg aaatgcctta atactacaat tttgtctgag acgcctgtgt ttttgccaaa
     7501 aacaactgag caaattcaaa tttcagagaa aatagaatct tttgaaaatc aattaaaaca
     7561 acttaataat cgaaaagcca aactccaatc cctcaaaaaa ggcttaatgc gggatttgct
     7621 aagtggaaag gtgagggtaa aaatataaac tttcaacatg aaaagtcaaa acattttatc
     7681 agcttattgc tttttggcgg ctctaaccga aaacaacaat gatttgtaca accacgtgta
     7741 cgttcctatg tgtaaacgag cattgagtga gtactcaaga aagggaaaag attatggaac
     7801 tcatttggac atcaaagaaa ctatacttag aatgtatggc atagatgttc cggaatcaat
     7861 tgtgtgtaaa ctaattcggg gttgtgaggc ttcactgtca agaagaaaaa aagaagaaat
     7921 taacttcaag gttcttgaaa atggaagaac ttttcaatta gaagcatggg tatttaagga
     7981 ttttgaagaa aaatttgata aaagtcaaag agaagcaaat gcgattgaaa gtctttttca
     8041 agaatatgta attaataaaa gaaatttcac agaggaaata ccttcatttg ctgattattt
     8101 atcaagatac aaaactcaat tgtcatcttt ttttgaaggg gaattcaaat ctattgaaag
     8161 aacagaagaa gaaacatttt tttatcatgt tgaattcttg gaagaattac aaaggtctaa
     8221 tcatactttt tttagaatag cccagaattt ataccttggt gcaatagtta caggtttttt
     8281 agagtcaggt tttgatttag acccaagaaa gccatcaaat gagtgtttct ttcttgacac
     8341 accagttcta ttgagagcat tagatttgca aagagcagaa gaaaccaacc ctatccttga
     8401 actaatatca ttaataaaca aaacgggcag tactccgaaa gttctttcaa taaccgttga
     8461 ggaaattcaa aaagtgataa atagtgcaat tgaaacgtat aataataaaa tatcagttac
     8521 tacaattaac gatgcttgtt taagaagaaa aaaagacaaa gcctggttaa tgacatttag
     8581 tacgaattta caaaaaaaca ttacaagtat tttgcagtta gagattgaaa atgtaggggt
     8641 tgattttatt aaagaaaacg agaaatgcgc cgacatccca gccctgcaag aattaagaag
     8701 atgtaagaat aatgcagccc atgatgtttt tgcctactta tatgttagaa aacttaggca
     8761 aacatctgtc tcaataattc aaaatgcaaa agtatggttt gtcacaacga atccttcttt
     8821 attagctttt aattctgata tgagacttgc taattcagta ccagaaattg ttttaccaga
     8881 catgcttacg agtttgcttt ggttaaaaga tccttcaaag ttagtggaag aagtaaaaaa
     8941 gattggatta aaagagttaa tgacaagtac tattttggag gaagttgcca gcaaagaatt
     9001 aattcatgaa tataatttac aaataaagaa aattgaagga attgatagtg attcgtacaa
     9061 tattttactt gaagcagtag cacattattc ggctaatagt attgagaaat ttattgagtt
     9121 agctgaacaa gatcaaatta aggcgagaga aaaagcattg caaattgtag aagatgaaag
     9181 gacaagaaaa gctgaaatac aacaaaaaat tgctgatgca ttgacagctg aagaaagaga
     9241 gaaatccgaa aaagaaattt ttgcaaaaaa acttgaagag tttgagaatc aacttagaga
     9301 atcaaatgaa gcagcaaaaa aagaaattga taagttaaat gaaaaggtaa cttcacatga
     9361 ggatattatt tccagacaag aaaaagctat tgatgaattt ggtaataaga ttgatgagca
     9421 agatgaacaa atttcaaatt taaaaaagca aataaaaaag gatacaaaaa aacttttaat
     9481 tggggttatt ggcgttataa tatttgttct tacgtttatt tttaaagaca atttaggcaa
     9541 ttggacttgg tttactgctg ttttatctgg ttcaggttgg atttgggggt ttggttcatt
     9601 ttgcataaat atgattaaaa tattgaaaaa aaattaatcc atggcaaaaa aagaatacaa
     9661 aatcggcaat aaatattcca caagagaatt gatcgaatta gccatcgaag aaagccataa
     9721 atctattcct gagcatactg ataaaaccga cccgcttgta ggtgcaatta ttgctactgc
     9781 cgatggtgaa ctattagcta ctgctcaccg tggcgaacta cgggagggcg agcattgcga
     9841 gtacacactg attgaacgca agttggttaa catgaacctg aaaggctgtg ttttgtatgt
     9901 tacacttgag ccatgtaccg atgaatcacg taaaaaaacc aagcggggtt gctctaccca
     9961 cattggcaaa gctcgtttaa gtaaggtata cattggagta gaagacccaa acccaaaaat
    10021 agcaggtgat ggtattaaag ccattcgcaa taaaggcatt ggagttgaaa tgttcccaag
    10081 cgatttacaa gagctaatct ggaaagacaa tgcgaaattc aaaaaagaaa aagaactgga
    10141 agcccttcaa gccaaattag atgaagcaaa accccaatta agcattttag aacaaactgt
    10201 cgtaggctca ggcattacaa gtttttcaga caaagcgatt aatcagtttg tcaaagaatc
    10261 aaaagctgaa ttcaactacc catcttccga ctttaatgaa tgggctcaag ggcttggatt
    10321 tattgaaaaa gatgccaaag atgatggctt tttacccact ggtttgggta ttatgctctt
    10381 tggcaaaact cccgaaaacg ctttccctca aaccatcttt aaggtggaaa ttaattatgg
    10441 cacaggaaag cccgaaataa aggatttcaa aggtcctttg gtttcccaat tacccgaaat
    10501 catagactac gttaaagata aagggctcaa gatgaccatg gaaaaatcga gtggtaaaag
    10561 agaagaaatt gccaactttc cctttgaggt gctgcttgag gccattgcca atgctgtaat
    10621 tcacagggat tattccattg aaggagcaac aaactattta tatatcg
//
LOCUS       DAOSPF010000092        10626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6231_length_10626_cov_7.358150, whole genome shotgun sequence.
ACCESSION   DAOSPF010000092 DAOSPF010000000
VERSION     DAOSPF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6231_length_10626_cov_7.358150"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PK252_13155"
     CDS             <1..619
                     /locus_tag="PK252_13155"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT15700.1"
                     /translation="LKIIEGFKNEKIKEYLTFSTINQNLTILGTRNFDKYYNYFRKHN
                     TDPAYSKEVAKTYAKKLLMAPGKPAPEFSCVDKDSNYISLKNFRGKYVYIDFWATWCS
                     PCMQELPEYIKLQNDYKGKNIAFVSISLDLDKNSWKKVIAENKSGAVNLFAPNGWEAD
                     VVKAYQIYGIPTFVLIDKDGNIIDAQAPRPSSVEIRKTLDKLLKK"
     gene            857..1663
                     /locus_tag="PK252_13160"
     CDS             857..1663
                     /locus_tag="PK252_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOT15701.1"
                     /translation="MKGQLILISSILLSLYFAAHAQKTQIKSQRTVITAQSQTNAPVK
                     AIKRGHKHKVRKKDNRPYLTINDVRNIDSLWGIDISHHQTEIDWNALEAEKPHFMFIK
                     ATQGIDIEDHKYSTYYAEAKKLGIPVGSYHFFTYKYDGKQQAEKFLSVAQNQSGDLLP
                     VLDAERTRHMPRNKYKITSELTAFIETVYEKLGHYPIIYCTYAYSQLYLNEEILKKCK
                     LWIADYKSKPKCNYVVWQATDRLKLAAIKEHVDLNFLNRDSCSLQEIFKP"
     gene            complement(1731..2813)
                     /locus_tag="PK252_13165"
     CDS             complement(1731..2813)
                     /locus_tag="PK252_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15702.1"
                     /translation="MRKIFILALIGIATQVSFAQVKDSVCKTHYGIAYPISTHGKDAA
                     NITNKFSLHLFTAYSAGVDGFEAAGVLSNVKGNVEGMQLAGVVNITEGTVKGIQGTWG
                     LNFTHGNIKGLQCSGMMNIAEKNVKGFQGAGCANIADSIKGFQGSGFINIANEVKGVQ
                     WSGFINYAKKVKGTQVSVFINIADSSDYPVGLVNIIKNGKKTLNISTNMQNDIMLSFK
                     SGGRKLYGVVGIGYNPFYKELEYNAHAGMGYHIPVSQKFTIDIEGIALGYLSKDDMDE
                     ADLKHNHHKNDSLGAASAMLNLAFDYQPFEHFGITAGPTLQYMFTRNKSKYDCINNTL
                     WNKENLDKNRFQALWVGFTASVYYRF"
     gene            complement(2960..4258)
                     /gene="icd"
                     /locus_tag="PK252_13170"
     CDS             complement(2960..4258)
                     /gene="icd"
                     /locus_tag="PK252_13170"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOT15703.1"
                     /translation="MEGKITIANGKLNVPDNPVIPFIEGDGTGPDIWAASVRVFDAAV
                     EKAYKGKRKIQWREVLAGEKSFKLNGNWLPQETLDVINEYLVAIKGPLTTPVGGGIRS
                     LNVALRQMLDLYVCLRPVRYFSGVPSPVKNPAAVDMVIFRENTEDIYTGIDWKDDLEL
                     TTKFLEWLAVNSPKDFEKIRFGNIQKSQEWQKQLEAGGAPKRDVKTMVGIGIKPVSYL
                     GTERLVKSAIEYAIEHKRKSVTFVHKGNIMKYTEGAFRDWGYQVAKELFGAVEIDGGP
                     WCKIPEGKPGAGIVIKDAIADITLQQVLTRPEDFSVIATLNLNGDYLSDALAAQVGGI
                     GIAPGANINYVTGHAIFEATHGTAPKYAGQDKVNPGSVILSGVMMFEYMGWKEAADLI
                     VKGLEKAIATKRVTYDFERQMQGATLLKCSEFGDEIIRQM"
     gene            complement(4283..6004)
                     /locus_tag="PK252_13175"
     CDS             complement(4283..6004)
                     /locus_tag="PK252_13175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT15704.1"
                     /translation="MEQYLVSARKYRPSTFDTVIGQQPITVTLKNAIRSNHLAHAYLF
                     CGPRGVGKTTCARIFAKTINCLAPTSDTEPCNNCESCKSFNEMRSLNIHELDAASNNS
                     VDDIRTLIEKVRIPPQIGKYSVYIIDEVHMLSAAAFNAFLKTLEEPPHYAIFVLATTE
                     KHKILPTILSRCQIYDFSRIKIEDTVNFLEKIAQKENVTYDKEGLTIIAQKSEGAMRD
                     ALSIFDQIVSFSEGKVIYDRVINTLNILDYDYYFRLIDTVMKSDITSALLILDEIINK
                     GFDLQNFLAGLSGHLRDLLVCKEPQTHSLLEVGANIAEKYISQTKQLPLDYLLDYMQI
                     VSQCELSYRAARNQRLHLELALVQLCNYGLKRKQREQEIANIAKIQAEAKAQPQVEKA
                     HASAPKTGTTAKAASSQPAFTSTPSLKQMMDQVQAPKANKTESEPTVQTNAKQTDITR
                     ENAYNEDQLVDAGRNYAVSIKESRPRMYSALIESNALKVKDGKLFVCLLNNAQKEYFI
                     KNIQSEMAHFVKNELKNDNIVIECEIQEVNEENKKAYTPYEKLQAMKAKNPAIDNLVQ
                     NLKLDFE"
     gene            6175..6372
                     /locus_tag="PK252_13180"
     CDS             6175..6372
                     /locus_tag="PK252_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15705.1"
                     /translation="METAILNGDSKSDMKLLLELAKKLGIKARIMSESEIEDMGLVDA
                     IKQGMTNEYVDNNEFLKKIRK"
     gene            6369..6638
                     /locus_tag="PK252_13185"
     CDS             6369..6638
                     /locus_tag="PK252_13185"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT15706.1"
                     /translation="MIVKIDKTFEKDVDKIKDKILLNKIADCIEIAQKSEDISSIKNI
                     KKLKGSNSFYRIRIGDYRLGIIWENNSILFIRCLHRKDIYKYFPK"
     gene            6654..7679
                     /gene="rlmN"
                     /locus_tag="PK252_13190"
     CDS             6654..7679
                     /gene="rlmN"
                     /locus_tag="PK252_13190"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT15707.1"
                     /translation="MNKEPLFGKTLFELQEICRELQLPSFTAKQLCEWMYKKQASQID
                     QMTNLSKDTRAKLLEKYDLGINSPVDVKVSKDGTKKYLYSVFDGKFIESAYIPEFDRA
                     TLCVSSQVGCKMACDFCMTGKQGFQGNLTPGEIVNQIRSLPEYERLTNVVYMGMGEPF
                     DNIDNVLKSLEILTSDYGYGWSPKRITVSSVGILQGLTKFIEQSKCHLAISLHTPFAD
                     QRLEIMPAERVNPIKEVVETIKKYDFGLQRRVSFEYIMFKGFNDTSAHIKETVKLLNG
                     LKCRVNLIGFHPIPDSSLKGSPKETMESFRDALNDKGITTTIRTSRGLDIEAACGMLS
                     TKKKKVE"
     gene            complement(7722..8036)
                     /locus_tag="PK252_13195"
     CDS             complement(7722..8036)
                     /locus_tag="PK252_13195"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT15708.1"
                     /translation="MDKEIVNIIRQYISTVSNQTPGFVTAYLFGSFAKGNQRPESDLD
                     IALIIDNLKDSDKFDIQVQLMLLASKFDSRIEPHPLSLQDLISDNPFVYEIKKSGIEI
                     SK"
     gene            complement(8486..9271)
                     /locus_tag="PK252_13200"
     CDS             complement(8486..9271)
                     /locus_tag="PK252_13200"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT15709.1"
                     /translation="MKKFFLAVIISVLTVVVHAIPSAPANATAVNDYAGFISPDFKAQ
                     LEQKLNNFADSTSTQIVVVIVNDLEGQDPNMYAYEIGQNWGVGQKGKNNGVVFLIKPK
                     NENGKGRVAISVAYGLEQTLTDALSKRIIENDVIPFFKEGQYEQGIDAGVNAIMKACM
                     GLYKAEPQKRNEKSAKETVIIFIIILIIFIVFAAKSKGRGSDGMTIGRGGSSTAGWFM
                     MGSMMGSAGRSHNSGWGGFSSGSGGFGGFGGGSFGGGGASGSW"
     gene            complement(9438..9878)
                     /locus_tag="PK252_13205"
     CDS             complement(9438..9878)
                     /locus_tag="PK252_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT15710.1"
                     /translation="MKASDFFTKEEKAALVAAIEKAEKNTSGEIRVHIENKCPIDVLD
                     RTVQVFTKLNMHKTQQRNGVLIYLAIEDKKFAIIGDAGINSVVPANFWDSVKEQMQAK
                     FVEGKFSEGICIGIEAVGEKLKAFFPYQSDDVNELSDEISFGKG"
     gene            complement(9984..10520)
                     /locus_tag="PK252_13210"
     CDS             complement(9984..10520)
                     /locus_tag="PK252_13210"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HOT15711.1"
                     /translation="MKTIRNLTIALLITAMGFVVTGCYGSFALTKKVYDWNGSLGDKF
                     VKEAVFLVLNIIPVYGVCTFVDAIVLNLVEFWTGSNPMAIKQGHNQINENLAVDVNGN
                     NAVIKDANGKELATMSFNEADNTWYANINGHVVKVMTVNTNDVSIYTTSGKVLNVTKS
                     DVNKASTYYLVNEYSAMK"
BASE COUNT         3358 a         2162 c         1781 g         3325 t
ORIGIN      
        1 attaaaaata attgaaggtt ttaaaaatga gaaaatcaaa gaatatctga catttagtac
       61 tattaaccaa aatttgacaa tacttggaac aagaaacttt gacaaatatt acaattattt
      121 cagaaaacat aatacggatc ctgcatactc taaagaagtt gccaaaactt atgccaaaaa
      181 gcttttaatg gccccgggaa aacctgcccc tgaattttca tgtgtggata aagacagcaa
      241 ctatatctca ctcaaaaatt tcagaggaaa atatgtttat attgattttt gggctacatg
      301 gtgcagccca tgtatgcaag aactgccgga atatataaaa cttcaaaacg actataaagg
      361 caaaaacatt gcatttgtta gtatttctct tgatttagac aaaaatagct ggaaaaaagt
      421 catagccgaa aacaaaagcg gggctgtcaa cttatttgct cctaacggat gggaagctga
      481 tgtagtaaaa gcataccaga tatatggaat ccctacgttt gtgctgattg acaaagatgg
      541 gaacatcatc gatgcacaag ccccaagacc atcatcagta gagattcgta aaacactgga
      601 caaacttttg aagaaataaa aaaagtcttg tttttggaaa cttggtagat cttgttacag
      661 attcatattt ttatcccaaa aagttaacat aaaaagaggt tgtaaattca gaaaaagaag
      721 cagcctcttt tgttttttat caggattatt ggcatcctta tcaaacaaat taaaaatttc
      781 tgaaacaaga cttggcaaag tcaacgataa atttttaaaa ttgcaagttt ataaaggatg
      841 acaacacttt ttttaaatga aaggacaatt aattctcata tcaagcatat tactatcatt
      901 gtattttgcg gcacatgcac aaaaaactca aataaaaagc cagcgaacgg ttataacagc
      961 tcaatcacaa accaatgctc ccgttaaagc tataaaacga gggcacaaac acaaagttcg
     1021 taaaaaagat aaccgcccgt atctaaccat aaacgatgtt agaaatatcg atagcttgtg
     1081 gggcatagat atttcgcacc accaaacaga gatagactgg aatgcactgg aagccgaaaa
     1141 gcctcatttt atgtttatta aagctactca aggaattgat attgaagacc ataaatacag
     1201 cacttattat gccgaagcaa aaaagcttgg aataccggtt ggttcgtacc attttttcac
     1261 ctataaatat gatggaaaac aacaagcaga gaaatttttg tcggtagccc aaaaccagtc
     1321 aggtgatttg ttgccagtac ttgatgccga gcgtacaaga catatgccac gaaataaata
     1381 caaaattacc tcagagttaa cagcttttat tgaaactgtt tatgaaaaac tggggcatta
     1441 ccctattatt tattgtactt atgcttatag tcagctatac ctgaatgaag aaatccttaa
     1501 aaaatgcaag ctttggattg ctgattataa atcaaaacct aaatgcaatt atgtagtttg
     1561 gcaagctaca gacaggctta agctggctgc tataaaagag catgttgacc tgaatttctt
     1621 aaacagagac tcttgctccc tgcaagaaat attcaaaccc tgacactcaa aaaagccgct
     1681 tcattttaat attgaaacgg cttcattagt tatgttgata tgcaaaagtt ttaaaaccta
     1741 taataaacgc tggcagtaaa acctacccat aaagcctgga acctgttttt atcgaggttt
     1801 tctttattcc acaaagtatt gttgatgcaa tcgtatttgc ttttattgcg agtgaacatg
     1861 tattgcaatg tagggcctgc tgtgatacca aaatgctcga aaggctgata gtcaaatgcc
     1921 aggtttaaca tagctgatgc agccccaagg ctatcatttt tatgatgatt atgtttaagg
     1981 tcagcttcat ccatatcatc tttagacaga tatcccaggg caataccttc aatgtctatg
     2041 gtaaattttt gagatacagg tatatgatat cccatacctg catgggcatt atattccaat
     2101 tccttgtaaa aagggttata cccaatacct acaacaccat atagctttct tccgcctgat
     2161 ttgaacgata gcataatgtc attttgcata tttgtcgata tattcagggt tttcttgcca
     2221 tttttaataa tattgacaag gcctacagga taatcactac tatctgcaat gttgataaaa
     2281 acggaaactt gggtaccctt tactttcttg gcataattga taaagccaga ccactgcacg
     2341 ccctttacct cattggctat gttaataaag ccactaccct ggaaaccttt aatactatcg
     2401 gcaatattag cacatcctgc cccttgaaac cctttcacat ttttttctgc aatattcatc
     2461 atccccgaac actgcaaacc tttaatattg ccgtgagtaa aatttaatcc ccatgtaccc
     2521 tgtatgccct ttacagttcc ttcggttatg ttgacaactc cggccaactg cattccttct
     2581 acattccctt ttacattact caaaacacct gcagcttcaa aaccatcaac accggcagaa
     2641 taagcggtaa ataagtgtaa tgaaaattta ttggtaatgt ttgcagcatc tttcccgtga
     2701 gtactgatag gataagcaat gccataatgg gttttacata cgctgtcttt tacctgggca
     2761 aatgaaacct gagttgcaat acctattaat gctaaaataa aaatcttcct catatccttt
     2821 tgtttttcaa tgagacaaag aaagttgagt ttaccctcaa gatttttatc tttttcgatg
     2881 aacaggcaat tttagcagat gaacaaataa aaaacgccag atgataagca cctggcgttt
     2941 tacgaatata ttgaatattc tacatctgtc tgattatctc atctccaaat tcagagcatt
     3001 tgagcaatgt agcaccttgc atctgtcttt caaagtcgta ggtaacacgt ttggtggcaa
     3061 tagctttttc aagcccttta acaataaggt cagcagcttc tttccatccc atatattcaa
     3121 acatcataac accagaaagg attactgagc ccgggtttac cttatcctgt ccggcatatt
     3181 taggtgcagt accgtgagta gcttcaaaaa tagcatgtcc ggtaacatag ttgatattag
     3241 ccccaggagc aatgccaata cctccaacct gtgcagccaa agcatcgctc aaataatcgc
     3301 cgtttaagtt gagggtagca ataacactaa aatcttcagg gcgtgtaagt acttgttgca
     3361 aggtaatatc agcaatagca tcctttataa caatgccagc gccaggtttt ccttcaggaa
     3421 tcttacacca aggccctccg tcaatctcaa cagctccaaa aagctcttta gccacctgat
     3481 acccccaatc gcggaaagca ccttcggtgt atttcataat attgcctttg tgaacaaaag
     3541 taacactttt gcgtttatgt tcaatggcat attcaatggc agactttaca agtctttcag
     3601 ttcctaaata acttacaggc ttaataccaa tgcccaccat agtttttaca tcacgtttag
     3661 gggcaccacc cgcctcaagt tgtttttgcc attcctgaga tttttggata ttcccaaaac
     3721 gaattttttc aaaatcttta ggagaattaa cagctaacca ttccaaaaac ttggtagtca
     3781 gctccaaatc atctttccag tcaataccgg tataaatatc ttctgtattt tccctgaaaa
     3841 taaccatatc cacagcagca gggttcttga caggagaagg cacgccagaa aaatagcgaa
     3901 caggacgtaa acatacatac aaatcaagca tctgacgaag tgccacattt aatgaacgaa
     3961 tgcctcctcc aaccggagta gtcaatgggc ctttaatagc tactaaatac tcatttatta
     4021 catctaatgt ttcttgcgga agccaatttc cattgagttt aaaagatttc tccccagcta
     4081 aaacctcgcg ccattggatt ttacgtttgc ctttatatgc tttctcaaca gcagcgtcaa
     4141 aaaccctgac cgaagcagcc caaatatcag gcccggtgcc atctccttca ataaatggta
     4201 tcaccggatt gtccggcaca ttcagtttac catttgcaat tgttattttt ccttccatta
     4261 taatgattat ttatttctgt tattattcaa aatcaagttt caggttttgt accaggttgt
     4321 ctatagccgg attcttagcc ttcattgcct gcagcttttc ataaggggtg taggcctttt
     4381 tgttttcttc attcacttcc tgtatctcac attctataac aatattatcg tttttaagtt
     4441 catttttgac gaaatgagcc atttccgact ggatgttttt aatgaagtac tctttttgcg
     4501 cattgttcaa aaggcaaaca aagagttttc catccttaac cttgagtgca ttgctttcaa
     4561 tcaacgcact gtacatcctg gggcggcttt ctttaatact tacagcataa tttcttccgg
     4621 catcaaccaa ttggtcttca ttataagcat tttctcttgt aatatctgtt tgtttagcat
     4681 ttgtttgtac tgtaggctcc gattcggttt tatttgcttt aggagcctgc acctggtcca
     4741 tcatttgctt caatgatgga gtactggtaa atgcaggttg tgatgaagcc gctttagcag
     4801 ttgtcccagt tttaggggca gaagcatgag ccttttcaac ctgaggctga gccttagctt
     4861 cagcttgtat tttagcaatg ttggcaattt cctgttcgcg ctgttttctt ttcaacccat
     4921 agttgcaaag ctgaacaagg gctaactcaa gatgtaacct ttgatttcgg gcagccctgt
     4981 aactaagttc gcattgcgac acaatttgca tatagtcaag caggtaatca agcggcaact
     5041 gtttggtttg cgaaatatac ttttctgcaa tgtttgcacc cacttctaac aatgaatgcg
     5101 tctgaggctc tttacacacc aacaaatcgc gcaaatggcc tgacaagcct gccaggaagt
     5161 tctgcaaatc aaagcctttg ttaattatct cgtccaggat aagcaaagca gaagtaatgt
     5221 ctgacttcat aaccgtatca atcagcctga aatagtaatc gtaatcgagg atattcagcg
     5281 tattgatgac ccggtcatat attacctttc cttccgagaa gctcacaatc tggtcaaaga
     5341 tagaaagtgc atcacgcata gccccttccg acttctgggc aataatggtt aagccttctt
     5401 tatcgtaagt aacgttttct ttttgggcaa ttttttccaa aaagttaact gtgtcctcaa
     5461 ttttaatcct gctaaagtcg taaatctggc atcgcgaaag tatagttggc agaatcttat
     5521 gcttttcggt agttgccagc acaaatatgg cataatgggg cggttcttcc aacgtcttca
     5581 gaaaagcatt aaacgcagca gcagacaaca tgtgcacctc atcgatgata tatacgctgt
     5641 acttacctat ttgcggcggg atacgcactt tctcaatcag cgtacggatg tcgtccaccg
     5701 agttgtttga agcagcatcg agctcatgaa tattgagcga gcgcatttcg ttaaacgact
     5761 tacagctttc gcaattatta caaggctcgg tatcagacgt tggggcaaga cagttgatag
     5821 tcttggcaaa aatacgggca caagttgtct ttcctacacc ccttgggcca cagaaaagat
     5881 atgcatgcgc caaatggtta ctgcgtatgg catttttcaa cgttaccgtg atgggttgct
     5941 gcccaatgac cgtatcaaat gtcgatggcc tgtattttcg tgcagagact aagtattgtt
     6001 ccatttttat actgctgatt ttatcctaca aacctactcg aatttttgca atagtacaac
     6061 tgtttcttac atcttgaaag ccttgcattt attgaccaaa acaactatat tattaataaa
     6121 gaacaatttc caaattctta ttttaatcat tatctttgtt gaaaattatc aaccatggaa
     6181 acagctattt taaatggtga ttctaaatca gatatgaaat tattgcttga attggcaaaa
     6241 aaactgggta tcaaagccag aataatgtca gaatctgaga ttgaagatat gggacttgtt
     6301 gatgctataa aacaaggcat gactaatgaa tatgttgaca acaatgaatt cttgaaaaaa
     6361 atcagaaaat gattgtcaag attgataaaa cgttcgaaaa agacgttgat aaaataaaag
     6421 acaaaatact actcaataaa atagctgatt gcatcgaaat agcccaaaaa agtgaagata
     6481 tctcttctat aaaaaatatt aagaaattaa aaggtagtaa tagtttttac cgcatacgta
     6541 ttggtgatta tcgattaggt attatttggg agaacaactc catcttattt atccgatgcc
     6601 tgcatcgaaa agatatttac aaatattttc ccaaataaac tataaccatt tccatgaaca
     6661 aagaaccttt atttggcaaa acattatttg agcttcagga aatctgccgg gagctgcaac
     6721 taccttcatt cactgcaaag caactttgcg aatggatgta caaaaagcaa gcctctcaga
     6781 ttgaccaaat gacaaacctt tcaaaagata cacgggctaa actgcttgaa aagtatgatt
     6841 tgggtataaa ttcacctgta gatgtcaagg tttctaaaga tggtaccaaa aaatatcttt
     6901 actcggtctt tgatggcaag ttcatcgaat ctgcttatat ccctgaattt gacagggcta
     6961 ccttgtgtgt ttcctcacaa gtaggatgta aaatggcctg cgacttttgt atgaccggaa
     7021 agcaaggttt tcagggcaac ctgacacccg gagaaattgt aaaccagata cgcagccttc
     7081 ctgaatacga aagattgacc aatgtagtgt atatgggcat gggcgagcct ttcgacaata
     7141 tcgacaatgt attgaaaagc ctcgaaatac tcacctctga ttatggatat ggctggtcgc
     7201 ccaaaaggat aaccgtttca agtgtaggca tactccaagg ccttaccaaa tttatagaac
     7261 aaagcaaatg ccacctggca ataagcctgc atacgccttt tgctgaccag cggttagaga
     7321 taatgcccgc cgaaagggta aaccccatta aagaagtggt tgaaactatc aaaaaatatg
     7381 attttgggct acaacgcagg gtatcgtttg aatacattat gttcaaaggt tttaatgata
     7441 catctgccca catcaaagaa actgtaaagc tcctcaatgg cctgaaatgt cgggttaacc
     7501 tgattggttt ccatccgatt cccgatagtt cattgaaagg ttcgccaaaa gaaactatgg
     7561 aaagctttcg cgatgcacta aatgacaaag gtattaccac taccatccgc acttcaagag
     7621 gtttggacat tgaagctgct tgtggaatgc taagtacaaa gaagaaaaaa gtggagtaag
     7681 aaatccttta ttaaacataa atagacgctt ttcagcataa tttatttact tatctcaata
     7741 ccacttttct ttatttcata aacgaaagga ttatctgaaa tcaaatcttg aagagataaa
     7801 ggatgcggct caatccttga atcaaatttt gaagctaata gcattaattg aacttgaata
     7861 tcaaatttgt cagagtcttt tagattgtct ataattaatg caatatctaa atcactttct
     7921 ggcctttgat tacctttagc aaatgaacca aataaatatg cagtcacaaa tcctggggtt
     7981 tgatttgaca ctgttgaaat atattgccta attatattaa caatttcttt atccattgtc
     8041 tgtttatttt aagattttca atccaaatag cagtatattc aggtgtacat ttttgttaaa
     8101 agctcatttt ataatcatca tatcgagcat taatattgaa agctgtaact gttacaaaga
     8161 ataatttttg ctcttcagat aagttcaagt tacatttttc ggccagtcgt aataaattat
     8221 gaataagtgg tggatagtcg ttattggtct taacataaag tgcttttaac aatttctcaa
     8281 tcattaaatg acctacaaac aatgcccaat taaatctttt agtctcaaac atggcaatca
     8341 ttgtgtcaaa gtcatcatct gaactatcag accagtactt tattaactta tccttatcta
     8401 aatcttttta tctatttctc aaaggtacaa aatttcaatt aaaccttatc tacctttgta
     8461 taagcttgtt tcatcaaaac aacctctacc aactaccact agctccgcca ccgccaaaac
     8521 tgccgcctcc aaaaccgcca aagccaccag atccggagga gaatcctccc catccactat
     8581 tgtgcgacct gcctgcagaa cccatcatac tgcccatcat aaaccagcct gcagtgctgc
     8641 taccacctcg tcctatggtc attccgtctg aacctctccc ttttgatttg gcagcaaaca
     8701 caataaaaat gatcaggatg ataataaaaa tgataaccgt ttcttttgca gacttttcat
     8761 ttcttttttg tggctctgcc ttatataagc ccatacaggc cttcattata gcatttacgc
     8821 cagcatcaat accttgctcg tactgacctt ctttaaagaa aggtattaca tcattttcaa
     8881 tgatgcgttt acttagggca tcagtaagag tctgctccaa cccataagca accgaaatag
     8941 ccacgcggcc tttgccattt tcattttttg gtttgataag aaatacaacc ccattgtttt
     9001 ttcctttctg tccaacaccc caattttgac caatttcata agcatacata ttggggtcct
     9061 ggccttccag gtcgtttaca attactacca ctatctgagt cgaagtggaa tctgcaaaat
     9121 tgttgagctt ttgctcaagc tgagctttaa aatcagggct tataaaacct gcataatcat
     9181 taaccgctgt tgcattggca ggagctgaag gtattgcatg taccacaact gtcaatactg
     9241 atattattac tgctaaaaaa aactttttca tctctttact ttgttgtgtt tagtgtttag
     9301 gagtcagggg ttagtaaggc ttcacttttg ccgtactttt caattacgaa ttatcaatta
     9361 aaaaatacgg gttaaaatag cttcttacca ttaaccatta accattaacc attaaccatt
     9421 aaccattaac catttcttca tccttttcca aacgagattt catcgctcaa ttcgttaaca
     9481 tcatcacttt gataaggaaa gaaggctttc agcttttctc caacagcttc aattccaatg
     9541 caaatacctt cagaaaattt tccttctaca aacttagcct gcatttgttc ttttacgcta
     9601 tcccaaaagt ttgcagggac aacactgttt atgcctgcat cgccaatgat agcaaatttc
     9661 ttatcttcta tcgcaaggta tattaacacc ccatttcgtt gttgggtttt gtgcatgtta
     9721 agttttgtaa aaacctgcac cgttctgtca agtacatcta tcgggcactt attttcaatg
     9781 tgtaccctga tttcgcctga agtatttttt tcagcttttt caattgctgc tactaaggct
     9841 gctttttctt ctttagtaaa aaaatcagat gctttcaatg cttttatttt tagttgaaaa
     9901 gttcggcaat ttaaaaagaa aaaacctgaa aacctatttt tttaaccagg ctttcaggct
     9961 tttctatgtt taatgtttca agcttatttc atagctgaat attcgttaac caaataatag
    10021 gtagaagctt tatttacatc acttttagta acattcaaca cttttcctga tgtggtataa
    10081 atacttacat cgtttgtatt gactgtcatt acttttacta cgtggccatt aatattggca
    10141 taccaggtat tgtctgcctc attgaagctc atagttgcaa gttcttttcc attagcgtct
    10201 tttattactg cattatttcc attaacatca actgccaggt tctcatttat ctggttatgt
    10261 ccttgtttaa tagccattgg gttgctgcca gtccagaatt ctacaaggtt aagtacaata
    10321 gcatcaacaa aagtacatac cccataaaca ggaataatgt ttaatacaag aaacacagct
    10381 tctttaacaa atttgtcgcc caagcttccg ttccaatcat aaaccttttt ggtcaaagca
    10441 aaagagccat aacatccggt tacaacaaaa cccatagctg taattaataa cgcaatggta
    10501 agatttctaa tagttttcat aatgatttta tttaaaatta tcaatagata aatttataaa
    10561 gaatttttct ttaggtgtca agtttcacaa ggttgtttat gataacttaa ttgcaaagat
    10621 aaaact
//
LOCUS       DAOSPF010000093        10507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6323_length_10507_cov_6.155090, whole genome shotgun sequence.
ACCESSION   DAOSPF010000093 DAOSPF010000000
VERSION     DAOSPF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6323_length_10507_cov_6.155090"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="PK252_13215"
     CDS             <1..1391
                     /locus_tag="PK252_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15712.1"
                     /translation="YGNFNGSMDEVKWYGRKLNDNEIGKLYQQSVQPELLYPNNNDLF
                     VSSKCTITWRPSPRLAAINLQYSVNNGIVWQNIAVGVNTDAGKFVWNVPAQGANDVLM
                     RIANPTSGKSISINKTRIVSDIKELDLQAFFPFTYNANDAGRTKYRPIVYGAATVKLS
                     SGEKMNGCLEFNGTTDKLVIENFATSQQFSLAFWIKTTDSIATICSLWGAGYIGINNT
                     ENQYKGKILAYIENPKANYTFFLDTIKINDGQWHFVALSVANDSMRLFVDTRQVGVSK
                     VLGAIDYDYNFFVVGNYDYNSSMAYSGLLDELRFYNRGIDKNTIEEIRKAMFTMVDNE
                     VVIIRETVTDTLIIRDTIRTVVYDTIFRNDTIIIRDTIRITGSKIIATPNSGNEKVLT
                     SFPNPSTGRFTLQLPANECIPVSIFDNQGRLLQNYPACTGNTVIEGLKPGTYFIRAIA
                     KDRPYTLKQIIK"
     gene            complement(1452..2045)
                     /locus_tag="PK252_13220"
     CDS             complement(1452..2045)
                     /locus_tag="PK252_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT15713.1"
                     /translation="MKTKMTINYQQILAQVRDYVQSRCLATTNQLHPAFYEFHLLVVE
                     KYALQLAEMLKADREVVQIAALLHDISAVLDFSTLPTHAKDSASIAREMLDACNLSDL
                     KKQNIIHCIEAHVKPLPFGADTAEAICISNADAMAFIAMPSYWIYFAYTINKMDYGVG
                     LQWYKERVDKNWLNLIRPACDMIYESYLHNNLLFEMN"
     gene            complement(2363..2797)
                     /locus_tag="PK252_13225"
     CDS             complement(2363..2797)
                     /locus_tag="PK252_13225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT15714.1"
                     /translation="METDKSLVLYANLELVNERLLFKGEVEGQEPVYIDYIPPLGDNL
                     GYTSLELLLLSISSCMGTAVLTFLRKMKKNISGITIKTKGIRHQEHPTGFKTIILDIT
                     LQSKDTSLQELNKVIKMGEETYCPVFAMVKVNVEIIITHTII"
     gene            complement(2961..4136)
                     /locus_tag="PK252_13230"
     CDS             complement(2961..4136)
                     /locus_tag="PK252_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT15715.1"
                     /translation="MTANTENTRQDYIDWLRVIAIFLLLLLHSAMPFFKWSDWHIHNA
                     ERSVILTHLVSFLHTWRMPLLFFISGFGTFYALKKRSARQYAGERTKRLFIPFIIGMF
                     LIVPPQVFIEKIGQYGNYFNFLPEMFNGTYPQGNISWHHLWFILYLFLYSLMLIPFFF
                     WYKSEKGNALKEKIYKLIARPGGVLLFVIPFFVVWYSLTPFFPNETHALFNDWRTFFY
                     NLLFFVFGYMIMSDPRIKDVLVSQRRLLLYAVFLVFIPWYSNMFLPWESYSWIYYDAE
                     SMLLAWFIICALIGYGAKYLNRSNKFLKYANGALYPFYIVHQTVIVIIGYYILQQNWG
                     IGLKYLVINVGTLVISLFLYEYIIRRTVVTRVIFGVKKPKLAESKTLVPEPVPVENK"
     gene            complement(4248..5411)
                     /locus_tag="PK252_13235"
     CDS             complement(4248..5411)
                     /locus_tag="PK252_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT15716.1"
                     /translation="MISFPRIDIFSLFILAGVFQGVILSFVYLHKANNRYKSNVYIGV
                     LFLVLTLIILEIFLNYSGLIFKVLWLDNFSEPGNFLMGPLMYLYVKASLKMEQKRVWP
                     HFICFGLYCLYHILYLIQPLDFRFDSVNEVYAYNLPYRQVNIVCDPDPLLLRHYLNEI
                     FGLHWLTYFVLSAYFLVKKYKAAGLKIYSMLSTEYTWVRNFIFHVGVSFILFITVKST
                     FGRDLGDYLVATYVAAIMYITTFYLIKTSIALKPLAENVEANASTVKYQKSSLKETDK
                     EAIIAKLESLMKDEKVFKNNLLSLSELAKRAGVASHHLSQVINETTEKSFFEYLAAYR
                     VEEAKNMLSDEKNNLTIEEIAEEVGYNSKSAFNKIFKKHTNLTPSEYREKSRK"
     gene            5427..6029
                     /locus_tag="PK252_13240"
     CDS             5427..6029
                     /locus_tag="PK252_13240"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT15717.1"
                     /translation="MTKVKRTCQKYNIHHSISYICTNIHYSTTMNFTLRPWNNGDIGS
                     LVKYANNKNIARYLTNAFPHPYTHADGEKFIKLFSQDNPTRVFAIDVENEAVGSIGIF
                     PQTDVHCKNAETGYWLAEPFWGKGIVTAAIKQMVEYGFNTFDIDRIFARPFGSNLASQ
                     RVLVKAGFKPEARFEKAIFKNGIYEDECYYAIRKNEMIKP"
     gene            6026..6289
                     /locus_tag="PK252_13245"
     CDS             6026..6289
                     /locus_tag="PK252_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOT15718.1"
                     /translation="MKSNLSQQINIGKDTETKLIEVGISSFEELKAIGAEQAYIRLHA
                     VDPGACLSLLCGLEGAIHGISWHKIPKERKEELKAFIKMVNKQ"
     gene            6384..7754
                     /locus_tag="PK252_13250"
     CDS             6384..7754
                     /locus_tag="PK252_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOT15719.1"
                     /translation="MDFKEVHLKQQGFFLTNQTKDVDYRKNQLKKLKELLKANEQKLF
                     DAIYEDSGKSEFETYLTELSLLYHEISLFEKKLKSWSKPRRVWTDIANMPARSYIIPE
                     PLGVTLVIGAWNYPYLLSLHPAACAIAAGNTVMLKPSELPSNTARVMTELINTNFPAE
                     LLHVVEGGVKETTELLELKFDKIFFTGSTNVGRIVYQAAAKHLTPVTLELGGKSPTFV
                     FADCNLAMTAKRLVWAKFINAGQTCVAPDYVLVEKSIETKFLEALKLEIEKYHADRKD
                     IGENYLRIINERNFDRLCRLIDPAKVYCGGYTDKAKRFIAPTVMNKVTFDDAVMQEEI
                     FGPILPVIPFTNLDEAVKTVKDRPKPLSCYVYSKDKKRIKKILHEISFGGGAVNDSIM
                     HLSNSNLPFGGVGASGMGSYHGKAGFDTFTHYKSIVDKPFWIEPNLKYPPYKKYKLKL
                     IKWILG"
     gene            7864..8313
                     /locus_tag="PK252_13255"
     CDS             7864..8313
                     /locus_tag="PK252_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15720.1"
                     /translation="MITELTRKGSFQVNYSKEEVFPLLCPKIEEKWIPGWKCQVIKSQ
                     SGYNEKGAIFKTETAFNTELIWCTNEYDIETGNIDFILFAKDKYVFRFNIKVVQNAPN
                     SSELTFTHSFSSLSNEGSALIEAYKNEDFQGRLNTLGMLIETYLKTN"
     gene            8431..9666
                     /locus_tag="PK252_13260"
     CDS             8431..9666
                     /locus_tag="PK252_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HOT15721.1"
                     /translation="MRNNRKPALGFIFITLLLDVIGFGIILPVIPKYISHLVGSDLSN
                     ASLYSGWLMFSFSIMQFLFSPILGSLSDRFGRRPILLISLFGFTINYLIVAFAPSIGW
                     LFVGRLLAGITGASFTTASAYVADVSTPEKRAQNFGLMSAAFGLGFIIGPVLGGFFSQ
                     FGMRVPFFVAAGLTFVNWIYGYIVLPESLPLRNRRRFDIKRANPVGSLIMLKKYPIVW
                     GFVGSIICVYIAQFATQSTWTFFTMEAFHWNEALVGYSLGIMGLLMVIVQGGLVRVTN
                     PLLGPVKSIYLGLAFYCISLVLIAFVPKGWMLFVLMVPFCLGGIANPAVQSIITNEVP
                     ANEQGELQGALTSLMSLTSIVGPILMTSLFSEFTSKHAPFYFPGAPFMMAALLVLLAT
                     ILAKKALSAIKVQAVPVRR"
     gene            complement(9843..>10507)
                     /locus_tag="PK252_13265"
     CDS             complement(9843..>10507)
                     /locus_tag="PK252_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT15722.1"
                     /translation="SIVAEIKKVADAGKNVVVLIDDAYFGLVYEDKVFTESIFVELAN
                     LSERVLAVKLDGPTKEDYVWGFRAGFVSYAIKGGTAELYEALENKTAGAVRGNISNIS
                     NLSQSLLYKVYTSASYEDSKKQKFEILKSRYNILRNELNKPGYSEYFEALPFNSGYFM
                     CITLKKGLVAEEVRKHLLEKYSTGVIVLGSVIRLAFSSVPAEKIPVLVENIYKACKDL
                     AK"
BASE COUNT         3409 a         2100 c         2014 g         2984 t
ORIGIN      
        1 cttatggaaa ctttaatggc tcaatggatg aggtaaaatg gtatggacga aagctaaacg
       61 acaatgaaat tggaaaactt tatcaacaat ctgtccagcc cgagcttctg taccccaaca
      121 ataacgacct gtttgtaagc agcaaatgta ccattacctg gagaccttcg ccccgactgg
      181 cagcaataaa tttacagtac tctgtcaata acggcattgt atggcaaaac attgctgtag
      241 gtgtaaatac cgatgccgga aaatttgttt ggaatgtacc cgctcaaggt gcaaatgatg
      301 tattgatgcg cattgccaac cccacttcgg gaaaaagcat atcaattaat aaaaccagga
      361 ttgttagcga tataaaagag ctcgatttac aagctttctt ccctttcact tacaatgcca
      421 acgatgcagg ccggactaaa tacaggccaa tcgtttatgg tgctgcaact gtaaaacttt
      481 cttctggcga aaaaatgaat ggttgcctcg aatttaatgg tacaaccgac aagctggtaa
      541 ttgaaaattt tgccacttca caacaatttt cgcttgcttt ttggataaaa actaccgact
      601 caattgccac aatctgttca ttgtggggag ctggctatat cggcatcaac aacaccgaaa
      661 accagtataa aggtaagatt ttagcatata ttgaaaaccc aaaagccaat tatactttct
      721 ttttagatac tattaaaatc aacgacgggc aatggcactt tgtggcttta tcggtagcca
      781 atgacagcat gaggttgttt gtcgataccc ggcaggtagg cgtgagcaaa gtattggggg
      841 caattgatta cgattataac ttttttgtcg tgggcaatta cgattataat tcctccatgg
      901 catacagcgg gttattggac gaactcaggt tttacaacag ggggattgac aaaaatacga
      961 tagaagaaat caggaaagcg atgtttacca tggtcgataa cgaagtggtt attattcgcg
     1021 aaacagtaac agacactttg ataatccgtg ataccattcg cactgttgtt tatgatacaa
     1081 ttttccgtaa tgatacaatc atcataaggg acacaatcag gataacaggt tcaaaaataa
     1141 ttgcaacacc taatagcgga aatgaaaaag tattaacaag tttcccgaac ccatcaacag
     1201 gaagatttac actccaattg ccagctaatg aatgcatacc tgtttcaata tttgacaacc
     1261 agggcaggct attgcaaaat tacccggcat gtacaggtaa tacagttatt gaagggctaa
     1321 aacctggcac ttattttatc cgggccatag ctaaagatag gccttataca ttaaaacaaa
     1381 tcataaaata ataaacgcct gtattctcta aaaaaaacgg ccatatcaac aagctggtat
     1441 ggccgttttt tttaattcat ttcgaacaac aggttgttgt gcaaatagct ttcataaatc
     1501 atatcgcaag caggacggat aaggtttagc cagtttttgt ccaccctttc tttgtaccat
     1561 tgcaggccaa caccataatc cattttgttg atggtgtatg caaagtatat ccaatacgaa
     1621 ggcatggcta taaatgccat ggcatctgca tttgaaatgc atattgcctc ggcagtatca
     1681 gcaccaaaag gcaatggctt aacatgagct tcgatgcaat gaataatatt ctgctttttc
     1741 aaatcagaca aattgcaagc atcaagcatt tcgcgtgcaa tagaagcact gtcttttgca
     1801 tgagttggta gcgttgaaaa atcaaggaca gccgatatat cgtgcaacaa agcggcaatt
     1861 tgcaccactt ccctgtctgc cttaagcatt tcggccaact gtaaagcata cttttcaacc
     1921 accaagaggt gaaactcgta aaatgccgga tgcaactgat tggtagtcgc aaggcaccta
     1981 ctttgaacat agtcccttac ctgggcaagt atttgctgat aatttattgt catttttgtt
     2041 ttcatacttt tatattttaa aatcaagtca aaattacttt acgtttttcc ctgatataaa
     2101 acataaattg ttgaatgaca gtttttaacc cttgaataaa aaaccgtttt acccaatatg
     2161 cagtaaagcg gcttaaccca ataaaatatg tctatagacc taagtaactg gagttcgata
     2221 taaaataatt atggcactat actaatatta atttttcttt tttttctgct tcgcagcctt
     2281 tgattaaaac tactgccaat acaagctctc ataaagggca tttaaaattg tatcaatgta
     2341 ttttatatta gtcttaatga tattaaataa ttgtatgcgt aatgataatc tctacattta
     2401 ctttaaccat cgcaaaaaca ggacaatatg tttcttcgcc catttttatc actttattca
     2461 gctcttgtaa tgatgtatct tttgactgta atgtaatatc aagaatgatg gttttaaaac
     2521 cggttggatg ctcctgatga cgtattcctt tagtttttat tgttattccg gaaatattct
     2581 ttttcatttt ccttaaaaaa gtaaggactg ctgtacccat gcatgatgaa atgctcagta
     2641 ataaaagctc aagcgaagtg taaccaaggt tatcgcccag cgggggaata taatcgatat
     2701 acactggctc ctgtccttcc acttctcctt tgaagagcaa tcgttcgttt accaattcta
     2761 gattggcata taacactaat gatttatctg tttccatgat gtttattttt atctggccca
     2821 aaaatattgg cattagcaat acttcataaa taaagaatcg tccaatgacc aaaattgatt
     2881 attgaacgac ctttacgatt aaataaaaag aatcaagaat caagaaccaa gaatcaagat
     2941 ttttagttta gaaaatagaa ttacttgttt tccactggca ctggctcggg aaccaaagtc
     3001 tttgattcgg caagcttagg cttctttacc ccaaatataa cgcgggtgac aactgtacgg
     3061 cgaataatat attcgtacaa aaacaagctg ataaccaatg ttccaacatt tataactaag
     3121 tattttagtc caatacccca attttgctga agaatataat acccaatgat tacaatcacc
     3181 gtttggtgta caatataaaa aggatacaaa gcgccattgg cgtatttcag gaacttgttg
     3241 ctgcggttca ggtactttgc gccatagcca atcaatgcac aaataataaa ccatgcaagc
     3301 agcatcgatt ctgcatcata gtatatccaa gaatacgatt cccaaggcag gaacatgttt
     3361 gaataccatg ggataaaaac aagaaaaaca gcatacaaaa gcaaccggcg ttggctaacc
     3421 agcacgtctt taatacgcgg gtcagacata atcatgtatc caaatacaaa gaacagcaag
     3481 ttataaaaga atgtgcgcca atcgttgaac aaagcatgtg tttcattagg aaagaaaggc
     3541 gttagtgaat accaaactac gaaaaacggg ataacaaaaa gtaaaacgcc accgggacgc
     3601 gcaataagct tgtatatctt ctctttcagc gcattgcctt tttcgctttt ataccaaaag
     3661 aaaaacggga ttagcattaa agaatataag aacaggtata agataaacca cagatggtgc
     3721 cagcttatat tgccctgtgg gtacgtacca ttaaacattt cgggcaaaaa gttgaaatag
     3781 ttgccatatt gcccaatctt ttcgataaat acctgaggtg gcactatcag aaacatgcca
     3841 atgataaaag gaatgaacaa ccttttggta cgctccccgg catattgcct ggccgaacgc
     3901 ttttttaaag cataaaatgt accaaaaccc gagataaaga atagcagcgg catccgccat
     3961 gtatgcaaga acgacactaa gtgggtgagg ataacactgc gctcggcatt atggatgtgc
     4021 caatcggacc atttgaaaaa cggcatagcc gaatgcaaca ataataaaag gaaaatggcg
     4081 attaccctga gccagtctat ataatcctgg cgggtatttt cagtatttgc tgtcatgatt
     4141 caaaaatttt agttaaacat ttttttttga cagcaaaact atgttgaccc caagcaaagc
     4201 cgaatgtgcg cctgcaaggg agcagtgtgc gcttataagc gtgcacgcta ttttctcgat
     4261 ttttcacggt attcagaagg agtaagatta gtgtgctttt tgaagatttt attgaaagcc
     4321 gacttagaat tgtaccccac ttcttcagca atctcctcaa tggtcaggtt gtttttttca
     4381 tcggacaaca tattcttggc ctcctcaacc ctgtatgctg caaggtattc aaaaaatgac
     4441 ttttcagtag tttcattaat tacctgcgaa agatgatgcg aagccactcc tgcccttttt
     4501 gccagttcac ttagcgacaa cagattgttt ttaaaaactt tttcatcttt catcaaagat
     4561 tccaatttgg caatgatagc ctctttgtcc gtttctttga gcgacgattt ctgatatttt
     4621 acggtgcttg cgttagcctc aacattctca gccaatggct ttagtgcaat agaagttttt
     4681 atcaggtaaa atgttgtaat atacattatt gcagccacat acgtagcaac aaggtaatcg
     4741 cccaggtcgc ggccgaaagt gcttttcacc gttataaata gaataaaact taccccgaca
     4801 tgaaatataa agttccgaac ccaggtatat tcagtgctaa gcatcgaata tattttcagt
     4861 ccagcggcct tgtatttttt aaccagaaaa taagccgaaa gcacaaaata agtaagccag
     4921 tgcaacccga atatttcatt caaataatgt cgcaatagca gtggatccgg gtcacatact
     4981 atatttacct gccgataagg cagattatag gcataaacct cattcaccga gtcaaaccta
     5041 aaatcaagag gctgaatgag atataaaata tggtacagac aatacaggcc aaaacaaata
     5101 aaatgcggcc aaacccgctt ttgctccatc ttaagcgaag ctttaacata caaatacatc
     5161 aaagggccca tcagaaagtt gccgggctcg gagaaattat ccaaccacaa caccttaaat
     5221 atcagcccgg agtaattgag aaaaatctcc aatataatca gcgtaagcac caggaacaac
     5281 acccctatat aaacatttga tttatagcgg ttattggcct tatgcaaata aacaaacgac
     5341 aaaataacgc cttgaaaaac gccggcgaga ataaataaag aaaatatgtc gattcgagga
     5401 aatgatatca aaacaaaagc ttttagatga cgaaagtaaa aagaacatgc cagaaataca
     5461 atattcatca tagcatatct tacatttgta caaacattca ttattcaacc accatgaatt
     5521 tcacattacg cccatggaac aatggcgaca tcggcagtct ggtaaaatac gccaataata
     5581 agaacattgc caggtatctg accaatgctt ttccgcaccc ttacacgcat gccgatggcg
     5641 aaaaattcat aaagctcttt tcgcaagaca accccactcg cgtatttgcc attgatgttg
     5701 aaaatgaagc tgttggctct atcggcatct ttccacaaac cgatgtacat tgcaaaaatg
     5761 ccgaaacagg atactggctg gccgaaccat tctgggggaa aggtattgtt accgctgcta
     5821 ttaagcaaat ggttgaatat gggttcaaca cctttgatat tgatagaatt tttgcccgtc
     5881 cttttgggag caaccttgcc tcacagaggg tattggtaaa agccgggttc aagcccgaag
     5941 cccgctttga aaaagccatt ttcaaaaatg ggatttatga agatgagtgc tattatgcta
     6001 tacgaaaaaa tgaaatgata aaaccatgaa aagcaacctg tcacaacaaa taaacatcgg
     6061 caaagacacc gaaacaaagc tgattgaagt gggcataagc agttttgaag agttgaaagc
     6121 cattggagca gagcaagcct atatacgcct tcatgctgtt gaccccggag cttgcctgag
     6181 cctgctatgt ggcctcgaag gggctataca tggcattagc tggcacaaaa tacccaaaga
     6241 acgcaaagaa gagctgaaag catttataaa aatggtaaac aagcaataat aggttgaaac
     6301 ttgcgaaaaa ccttccaggt ttttgaaacc tggaaggttt aaggtacaaa aatcaacaac
     6361 ttcaacccaa gactaaaaac aatatggatt tcaaagaagt acacctcaaa cagcagggct
     6421 ttttcctgac caatcaaacc aaagatgttg attacaggaa gaaccagcta aagaagctaa
     6481 aagagctctt gaaagctaac gaacaaaagc tttttgatgc tatttacgaa gactctggca
     6541 aatctgagtt tgagacctat ctgacagagc tgtcacttct gtaccacgaa atatcgcttt
     6601 ttgaaaaaaa gctcaaaagt tggagcaagc cccggcgtgt ttggacagat atagccaaca
     6661 tgcctgcccg cagttatatc attcctgagc ctttgggtgt tacccttgta attggcgcct
     6721 ggaactatcc ttatttactg tcattgcacc cggctgcatg tgctattgcg gcgggcaata
     6781 ccgtcatgct aaagccaagc gaattgccat cgaatacagc cagagtgatg actgagctta
     6841 tcaatactaa tttcccggcc gaattgttgc atgttgtcga aggcggagtt aaagaaacca
     6901 ccgagcttct cgaactgaaa ttcgataaga tatttttcac cggaagcact aacgtgggaa
     6961 gaatagtata tcaggctgct gcaaaacatc tcacacctgt aacgctcgaa ttggggggca
     7021 aaagtccaac attcgttttt gccgactgta atttagctat gacagccaag agattggtat
     7081 gggcaaagtt catcaatgcc gggcaaacat gcgttgcccc tgattatgtg ctggtcgaaa
     7141 agtcaataga aacaaagttt ttagaagctt taaaactgga gattgagaaa taccacgctg
     7201 accgaaaaga cattggcgaa aactacttac gaatcatcaa cgaaagaaat tttgaccgtc
     7261 tgtgcaggct tattgacccg gcaaaagtct attgcggagg atacacggac aaagcaaaac
     7321 gcttcatagc cccaacagta atgaacaagg taacttttga tgatgccgtg atgcaagaag
     7381 agatttttgg gcccattctg ccggttattc ctttcacaaa ccttgatgaa gctgtaaaaa
     7441 cagttaaaga ccgtcccaaa cccttgtcgt gctatgtata ttcaaaagac aaaaagcgga
     7501 taaaaaagat tttgcacgaa atatcctttg gcggcggcgc agtaaacgac agtatcatgc
     7561 atctgtccaa cagcaacctg ccttttggcg gggttggtgc cagtggcatg ggtagttacc
     7621 atggcaaagc cgggtttgat acttttacgc attataaaag cattgtcgat aagccttttt
     7681 ggatagaacc aaatttgaaa tacccgccat acaagaaata taagctgaag ctgattaagt
     7741 ggatattggg ataataccat tcaacctgat attttagcca tttaagccct tttttcggga
     7801 atttacctac ttaatatttc tcggttttac actgaaactt acttttataa aaaagtgtag
     7861 aacatgatta cagaattaac aagaaaaggt agtttccagg taaactactc aaaagaagag
     7921 gtttttccat tgttatgccc aaaaatagaa gaaaaatgga ttcccggctg gaagtgccag
     7981 gtaataaaat cgcaaagcgg gtacaatgaa aaaggggcta tttttaaaac agaaacagca
     8041 ttcaacacag agctaatctg gtgtacaaac gagtatgaca tcgaaaccgg gaatatagat
     8101 tttatccttt ttgcaaaaga caaatacgtt ttcaggttca acatcaaagt ggttcaaaat
     8161 gccccaaata gcagcgaact gacttttaca cattcgttct cctcattaag taatgagggt
     8221 tcggcattaa ttgaggctta taaaaacgaa gattttcaag gaaggttaaa tactttagga
     8281 atgcttattg aaacctatct gaaaacaaac taaggttcat tattaaaata gcttattaag
     8341 gcatttatcc attaaaaaaa ggtattcacc gatttagatt gtcccacaaa tttgcggttt
     8401 tgttcatttg caggtataaa tttaaaaaaa atgagaaata atagaaaacc agcgctaggg
     8461 tttattttca taaccctgtt attagatgta attggatttg gtattatatt gccggttatt
     8521 cccaaatata tcagccattt ggtaggtagc gacttaagta atgcgtcgtt gtatagcggt
     8581 tggctaatgt tttccttctc tatcatgcag tttttgtttt cgccaatact gggaagcctc
     8641 agcgaccgtt ttggccggcg ccctatcctc ctgatttcac tttttggatt taccattaat
     8701 tacctgattg ttgcatttgc tccatctatc gggtggctat ttgtaggacg attattggca
     8761 gggattacag gtgccagctt cacaacagca tcagcatatg ttgcagatgt gagcactcct
     8821 gaaaaacgtg cacagaactt tggtttaatg agtgctgcct ttggcttagg cttcattatt
     8881 ggacctgtac tgggaggttt ctttagccaa tttggaatgc gtgtaccatt ttttgttgca
     8941 gcaggattga catttgtcaa ctggatttac ggttatattg ttttgcccga atcattgcca
     9001 ttgcgcaacc gtcgccgctt tgacataaaa cgtgcaaatc cggtaggctc attgatcatg
     9061 ttaaaaaaat atccaatagt ttggggattt gttggatcaa taatatgcgt atatattgcc
     9121 cagtttgcaa cacaaagtac ctggacattc ttcaccatgg aagcattcca ttggaacgaa
     9181 gcccttgtag gatattcgtt aggaataatg ggattgttga tggtaatagt acaaggaggc
     9241 cttgtaagag tgaccaatcc attgcttggc ccggtaaaat caatatattt agggttagcc
     9301 ttttattgta taagccttgt ccttatagct tttgtcccaa aaggctggat gttgtttgtg
     9361 ctgatggtgc cattctgcct tggaggtatt gcaaaccctg cagtacagtc tataataacc
     9421 aatgaagtcc cggccaatga acaaggagag ttacaagggg cattgaccag cttaatgagc
     9481 ctgacctcta ttgttgggcc aatactaatg accagccttt tctccgagtt tacctcaaaa
     9541 catgcaccgt tttatttccc cggggcacca tttatgatgg ctgcattgct ggtattactt
     9601 gcaactattc tggcaaaaaa agctttaagc gcaataaaag tgcaggcggt gccagttagg
     9661 agataatatg atgtaaaact gacccgacag cgtctttcag atttgtcagc gtttttttta
     9721 ataattaact aaacgacatt gaattacaat ttctaaatca acttcctttt ttaagaagtc
     9781 aaaaaacaag agccaaaact ttttcagatt tggctcttgt ttttacgatt aataaagcat
     9841 tattacttag ctaagtcttt acatgcttta taaatatttt caaccaagac aggaatcttc
     9901 tctgccggca cagatgaaaa ggccaggcga attactgaac ccaacactat cacgcctgtg
     9961 ctgtattttt caagtaagtg tttgcgcact tcttcagcta ccaagccttt tttgagtgtt
    10021 atacacataa aatagcccga attgaagggc agtgcttcaa aatattcgct gtatccgggt
    10081 ttattcaact cgttacgaag aatgttatag cggcttttta gaatttcaaa tttctgtttt
    10141 ttagaatctt cgtacgaagc agaagtataa accttataca atagcgattg cgaaaggttg
    10201 ctgatatttg aaatattgcc acgaacagcc ccggcggttt tattttccaa tgcttcatac
    10261 agttcagcag ttccaccttt gatggcataa gaaacaaagc cagcgcggaa tccccaaaca
    10321 taatcttctt tggtaggccc atcaagcttt acagcaagaa cacgttctga aagatttgcc
    10381 aattcgacaa aaatagattc agtaaaaacc ttatcttcat acaccaaacc aaaataagca
    10441 tcgtcaatta atacaaccac gtttttgcca gcatcggcca cttttttaat ttcagctaca
    10501 atagact
//
LOCUS       DAOSPF010000094        10211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6559_length_10211_cov_6.986707, whole genome shotgun sequence.
ACCESSION   DAOSPF010000094 DAOSPF010000000
VERSION     DAOSPF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_10211_cov_6.986707"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..700)
                     /locus_tag="PK252_13270"
     CDS             complement(20..700)
                     /locus_tag="PK252_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT15723.1"
                     /translation="MKNENILLRCIAVEPDDTALFALSESLKMIPYIELKNTFSNPFD
                     AFKYLNSNDIDLVFASIEMTENLNGIQFVNCMKSKPMVVFISNDERLASEAFNADAID
                     YLIKPFSFERLLKATNKASIHLAGTAQIESNALANNIQTQQNLFIKTDYKILKLKLNE
                     ILFFEGYNDYVKIHTSDAKPILSHVSLRVLEEKLPPNEFIRVHRSYIISLNKIDTITS
                     VPLKSGRY"
     gene            complement(951..2240)
                     /locus_tag="PK252_13275"
     CDS             complement(951..2240)
                     /locus_tag="PK252_13275"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOT15724.1"
                     /translation="MKLFKYAIFIALPFLVSNCAAIFYGRNQKVYINSEPAGASLFVN
                     GVDTRKTTPCTIKVKRRQPEGFINKKNEIVYVLKKENYYDGEFRDYAKKDYLPVYLDA
                     IYITPGIFDLLVGASTKTFTKNVSVKLNEKGKTIVKTDTVVKKEVVYVNAGTKEGKYV
                     FERKSDVDIDIPEVGKEKENRYALIIGNEDYSSRQIDLNTEINVDFARNDASAFKEYA
                     IKVLGIPENQVIFMLDATTGQMKQGISKLNLIIKNTDGEAEVFVYYAGHGLPDEVTKE
                     PYLMPVDVSGKNAADGIKLKDMYDKLTEFPSKRITVFIDACFSGGARNQGLLAARGVK
                     VKAKEAVNNGNIIVFSASSGEQSSLPYKAKEHGFFTYFLLKKLKETQGNVNYSELSAY
                     LKKNVSLQSVLLNDKEQTPQVNVSQSMIDAWGNISIK"
     gene            complement(2260..3093)
                     /locus_tag="PK252_13280"
     CDS             complement(2260..3093)
                     /locus_tag="PK252_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15725.1"
                     /translation="MNRLTYSFLLLIFMAFIPLKAQKIVRIKQAVGQYTLPAKSDISE
                     KTAYNKAVTEAKLNALRKAGVTENIASSEVVSTNQKGSDFKQDLNSILTVELDGAILN
                     DSVIQENKSIDEFGNTKYSVTISADVIIYEKKADPAFLFDVEGVKEYYENNDPVRFTF
                     KPYSNGYLKIFNFTENEISLLYPYFEKANSPVNDKTDFQFTADQAYTFPLNNAIDAYY
                     METKLPREFNYLVFVYIKENIPFRGKMSFKNVLSWIYSISPDKRRVQFLNFVIVNKQA
                     E"
     gene            3276..3974
                     /locus_tag="PK252_13285"
     CDS             3276..3974
                     /locus_tag="PK252_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOT15726.1"
                     /translation="MIKTFIYSIVLICISISLSGQSATGIATYYDDKFDGHNTASGEV
                     FDQNKLTAAHRNLPFGTKLKVTNLENSKSVIVRVNDRGPFVDSRLIDLSRSAAEMLDF
                     VVAGMARVRIDVVPAEDVTVSSEPNSSVKYKVDDKRVDFPGEYYELDIKYSHPSGFAV
                     QVGSFTELANLLRMAQELRKIHGRNLIVQVTDVDGKKVHRLLVSGFDSRAAAESEASR
                     LKNDFRDCFVVELK"
     gene            complement(4131..4994)
                     /locus_tag="PK252_13290"
     CDS             complement(4131..4994)
                     /locus_tag="PK252_13290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT15727.1"
                     /translation="MQKHTVKQPCTLIDFLGSEYPQTSKTKLKKWIENGRVMVNDHPA
                     GLYAHKLREGDLVQVTDGSSTSKSIPFDILYEDQHIIAIDKPAGIATVATDNQPCIFR
                     LISSLIKAEKGANKVFLVHRLDKEASGVLIFAKSRAAMEIIKDNWHKNDKKYLALVEG
                     NIQPEQGTIRSYLREGALQMVYECKPGEEGKLAVTHFRLQQTIGKYSLVEVSIETGRK
                     HQIRVHLSGIGFPIVGDTKYGKPDGFKRRLRLHAYWLTIEHPFSKETLTIQSKLPKGF
                     TRVEPRNENYK"
     gene            complement(5004..6269)
                     /locus_tag="PK252_13295"
     CDS             complement(5004..6269)
                     /locus_tag="PK252_13295"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT15728.1"
                     /translation="MEKYDIIVIGGGAAGLLAAGRAAELGAKVLIIEKMERPGRKLLI
                     TGKGRCNITNIAPIEAFLEHIYPNPDFVRQAFNVFFASDIIDLLNKFGVPTVIERGGR
                     VFPKSNSARDVVDALVKYVKRYNVQIIQGARVTQVNTANKKATGIDYIHSNQTKTVIS
                     DRVILCTGGCSYPATGSSGDGYKIAGELGHTIVKPLPSLVALEVSDKNVKELEGLNLR
                     NVTATVLLNNKKQQSLFGEMLFETFGLDGPIILSLSRFVVEKLQNKANKISINIDLKP
                     ALDEAQLEARLLRDFNTLGRKPIGIAFKELLPQQLISFFINRLGIEPFKPCSQISAKE
                     RQAIIHLLKNFEFEITGYRPFTEAIITAGGISTKEIDPKTMQSKLIENLYFAGEIIDL
                     DADTGGYNLQIAWSTAILAATSATQQKNE"
     gene            complement(6269..7594)
                     /locus_tag="PK252_13300"
     CDS             complement(6269..7594)
                     /locus_tag="PK252_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOT15729.1"
                     /translation="MQKVIKFNIDQIYTFRAKLIELAEKYETFLLLDSCPDKKNFFPF
                     GRFDMLAGIGSVEDYSHSAQYSDLSFIESINKNWILGHISYNYKNQTIGYTINDNINH
                     IGFQNFYFFKPRFLIKLCGREVELQYSPETEINQLKYVIDTLTSITLIKKKETYSHIE
                     IDFIKKSEYINVIHQLQKHIARGNVYELNYCIDFHATNACIDPYNLYLELTQISPAPF
                     SAFYKQKDKFCISASPERFIQKNGKKIISQPIKGTAARADNPTKDGEIKEELLANDKE
                     RAENIMITDLVRNDMAVFAEKKSVKVDELCQCYSFEHVHQLISTISCNVKDETSFNDI
                     INAVFPMGSMTGAPKFNAVKYSDEFEAFNRGLYSGAIGYIDPDMNFDFNVVIRSILYN
                     STNKHLSIPAGSGITAQSIPENEYDECITKVKALINLINKQEPQFNSGQ"
     gene            7801..8043
                     /gene="rpmB"
                     /locus_tag="PK252_13305"
     CDS             7801..8043
                     /gene="rpmB"
                     /locus_tag="PK252_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT15730.1"
                     /translation="MSKICQITGKTSMIGNNVSHSKRRTKRSFDINLFTKKFYLEEEK
                     RWIVLRVSAAGMRVINKNGLKAALDSAVEKGIILSY"
     gene            8065..8247
                     /gene="rpmG"
                     /locus_tag="PK252_13310"
     CDS             8065..8247
                     /gene="rpmG"
                     /locus_tag="PK252_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT15731.1"
                     /translation="MAKKGNRIQVILECTEHKESGMPGTSRYITTKNKKNTPERLELK
                     KYNPILKKVTVHKEIK"
     gene            8255..8407
                     /locus_tag="PK252_13315"
     CDS             8255..8407
                     /locus_tag="PK252_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOT15732.1"
                     /translation="MAKKVVATLKTGLGKNFTRVIKMVKNPATGSYGFKEDVMHNEEI
                     KDYLAK"
     gene            8553..9506
                     /gene="ftsY"
                     /locus_tag="PK252_13320"
     CDS             8553..9506
                     /gene="ftsY"
                     /locus_tag="PK252_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT15733.1"
                     /translation="MGLFGTKKNNDTLSSGLNATRQSLLSKLSRAIVGKSKVDDEVLD
                     NLEEVLISSDVGVETTIRVIERIEKRVAKDKFVGISELDTIMKEEIASLLSDNQNDVA
                     DNFFESELPKPYVILVVGVNGVGKTTTIGKLANRFKKAGKTVILGAADTFRAAAVDQL
                     TIWSERVGVEIVKQGMGADPASVAYDTVAAAIARNVDVAIIDTAGRLHNKVNLMNELT
                     KIKNVMRKLCPDAPHEVLLILDASTGQNAFEQAKQFTSATEVNALAVTKLDGTAKGGV
                     VLGICEHFKVPVKYIGVGEKIDDLQLFDKVEFVERLFDKKG"
     gene            9513..>10211
                     /locus_tag="PK252_13325"
     CDS             9513..>10211
                     /locus_tag="PK252_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT15734.1"
                     /translation="MINLLHNITQTAMKAGRRPGVIKYKKYTGYSAARHYRDLLTQAK
                     HEIKNSILIVFGILSAGFGLEGFLIPNSFIDGGVTGISLLFSKITNLPLSLFLIIINI
                     PFVIVGYYQIGKQFAFKSAAAIVGLSVCVIFVHYPVITSDKLLISVFGGFFLGAGIGL
                     AIRGGGVLDGTEILSLYLSKKIGLSIGDIILIINILIFSVAAYLLGIESALYSVLIYL
                     SASKTVDFLVDGIDE"
BASE COUNT         2944 a         1764 c         2040 g         3463 t
ORIGIN      
        1 agccaaattt gtttaatttt taataacgtc ccgattttaa tgggacacta gtgatcgtat
       61 ctattttatt aagcgaaatg atgtacgacc tgtgcaccct gataaattca tttggaggca
      121 gcttttcttc caatacccgc aggcttacat gcgataaaat tggcttggca tctgaagtat
      181 ggatttttac ataatcgtta taaccctcaa agaagagtat ttcgttcaat ttcaatttta
      241 ggattttata atccgttttt ataaaaaggt tttgttgggt ttgaatattg ttggccaagg
      301 cattgctttc aatctgggcg gttccggcca aatggataga agctttgttg gtagctttga
      361 gcaaacgctc gaacgaaaag ggttttatca gataatcaat ggcatcggca ttaaaagctt
      421 cagatgcaag cctttcatcg ttggaaataa acaccaccat tggttttgat ttcatgcagt
      481 tgacaaactg aatgccattg aggttttcgg tcatttcaat actggcaaaa actaaatcga
      541 tgtcattgga gttcaaatat ttgaaagcat caaaagggtt ggaaaaagta tttttcaact
      601 caatgtaagg gatcattttc aggctttcag aaagggcaaa cagggctgta tcatctggct
      661 ctacggcaat acacctaagt aaaatatttt cgtttttcat caatgcgttt ttggattttc
      721 aaaaaaatag acgcacaaaa gaaatgattt attgcatccg atgttgagct tttaacactt
      781 tttagcttaa aaatcaacta tataacagtt gttaagaaat gggtagagta gagttgccta
      841 tttatattta caaaagggca atatttagta aaccttacag gtttactaaa cctgtaaggt
      901 ttctggggtg taatccccca accttctaat taacaaacag ctatcatagg ttatttgata
      961 ctaatgttcc cccaagcgtc tatcattgat tggcttacat taacctgagg ggtttgttct
     1021 ttgtcgttca gaagcacgga ttgcaggcta acattctttt tcaggtaagc cgacaactct
     1081 gaatagttta catttccttg tgtttctttg agtttcttga gcaagaaata tgtaaagaag
     1141 ccatgttctt tggctttgta gggtaatgaa gattgttccc cagagctggc cgagaacaca
     1201 attatattgc cattgttaac agcttctttg gcctttactt tcacgccacg agcagccagt
     1261 aagccttggt tacgggcccc tcccgagaaa caggcatcaa taaacacggt aattcttttt
     1321 gaaggaaact cagtaagctt gtcatacata tcttttagtt taatgccgtc agctgcattt
     1381 ttgccactca catcaaccgg catcaaataa ggttctttgg taacctcgtc aggcaaaccg
     1441 tgtcctgcat aatatacaaa aacctcggct tcaccatctg tgtttttgat tatcaggttg
     1501 agctttgata tgccctgttt catttggcct gtagtggcat ctaacatgaa aatgacttgg
     1561 ttttcaggta tccccagtac ttttatggca tattccttaa aagcactggc atcattacgg
     1621 gcaaagtcaa cattaatctc ggtatttaag tcaatttgac gagagctata gtcttcgttg
     1681 ccaattatca gggcatatct gttttctttc tctttgccaa cctctggtat atctatatct
     1741 acatcagatt ttctttcaaa aacatatttg ccctcttttg ttccggcatt gacatatact
     1801 acttcttttt ttaccacagt atcagttttg acaatggttt ttcctttttc attgagcttt
     1861 accgaaacat tttttgtgaa agtcttggta gaagcgccca ccagaaggtc aaaaataccc
     1921 ggggtaatat atatagcatc cagataaacc ggcaggtaat ctttcttagc atagtccctg
     1981 aattcgccat catagtagtt ttcttttttc aaaacataca ctatttcatt ttttttgttt
     2041 ataaaacctt cgggttgtcg tctttttact tttatggtac atggtgttgt ttttcgtgta
     2101 tctacgccat ttacaaataa actggccccg gctggctcag agtttatgta cactttttgg
     2161 tttcgaccgt aaaatattgc tgcacagtta cttactaaaa atggaagggc gataaaaata
     2221 gcatatttga aaagcttcat tggtattttt ttttttggtt tattcggctt gcttattgac
     2281 tattacaaaa ttcaaaaact gtactctcct tttatcaggg ctgatgctgt atatccatga
     2341 aagtacattt ttgaaagaca tttttcccct aaaggggata ttttctttta tgtaaacaaa
     2401 aaccaggtaa ttgaactcgc gaggtaattt tgtttccatg taataagcat ctatggcatt
     2461 gtttaaagga aatgtatagg cctggtcggc agtaaactga aaatcagttt tgtcgttaac
     2521 tggcgaattg gctttttcaa aataagggta taacaatgaa atttcatttt cggtaaagtt
     2581 gaaaattttc agataaccgt ttgaataagg cttaaaagtg aatctgacag ggtcattgtt
     2641 ttcataatat tcttttaccc cttctacatc aaagaggaaa gcagggtcag cttttttttc
     2701 gtaaataatc acatcggccg aaatagttac actgtatttt gtatttccaa actcgtcaat
     2761 agatttattt tcctggataa cactatcgtt gagaatagct ccgtcaagtt ctacggtaag
     2821 tatagaattg aggtcctgtt taaagtcgct tcctttttgg ttggtagata ccacttctga
     2881 agaagcgata ttttcagtaa cgccggcttt gcgcagtgca ttaagttttg cctcggtaac
     2941 agctttattg taagcagttt tttcgctaat gtctgatttg gcaggcagcg tgtattggcc
     3001 aacagcttgt tttattctga caattttctg ggctttcaat gggataaagg ccatgaaaat
     3061 aagaagcaga aagctgtacg ttaatctatt catttgtcaa agtgttttga taacaaggaa
     3121 gccaaaatta taaataatct gacagatgta aaagttgaaa atacaggatg ttaaaaactt
     3181 atgttgtgtt gaaatactta agctacttta taatcttttt gctcaggctt gcctgatatg
     3241 ttgtattgtt gtattttggc ttaaaaatta aaaccatgat aaagaccttt atttattcaa
     3301 ttgttttgat atgcatttcg atatctctaa gcggacaatc ggctacaggt attgctacat
     3361 attatgatga caagtttgat ggccacaata ctgccagtgg tgaagtattt gaccagaata
     3421 aacttactgc ggcacatcga aatttaccat ttggcactaa actaaaagtt accaaccttg
     3481 agaatagcaa aagtgttatt gttcgggtca atgaccgtgg gccttttgtt gatagccggc
     3541 ttattgattt gtccagatct gctgcagaaa tgcttgactt tgttgtagca ggcatggcca
     3601 gggttaggat tgatgttgtg cctgctgaag atgtaacagt ttcttctgag cctaattcat
     3661 cggtaaaata taaagtggat gacaaaaggg ttgattttcc tggcgaatat tacgaactgg
     3721 atattaaata cagccatccg tctggttttg ctgtgcaggt gggtagtttt accgagctgg
     3781 ccaatttgct gcgtatggca caagagctca gaaaaattca tggccgcaac ctgattgttc
     3841 aggttactga tgttgatggg aaaaaagtgc accgcttgtt agtatctggc tttgatagca
     3901 gggctgctgc tgagagcgag gcaagcaggc tgaaaaatga ttttcgcgat tgttttgtgg
     3961 ttgagttaaa gtaattattt ctgccagaca atagtattaa taacttaatt aatgttgttt
     4021 agttaaggtg ctgacaggtt ctgcgaacgt tgtcaggtta gatgtgaaac taacccgaca
     4081 gcgtctttca ggcttgtcgg cgttttttta atatttaaaa cgacattgaa ttacttataa
     4141 ttctcgttgc ggggttcaac cctggtaaaa ccttttggca gttttgattg tatggtaagg
     4201 gtttccttac tgaagggatg ttcaatagtc agccagtagg catgaaggcg aaggcggcgt
     4261 ttgaaaccgt caggcttacc gtatttggtg tcgcctacta ttggaaatcc aatacctgaa
     4321 agatgtacac gtatttgatg tttacgtcct gtttcaatac ttacttctac aagtgaatat
     4381 ttaccaatgg tctgttgcag gcgaaagtga gtaacagcca gtttaccttc ttctccgggc
     4441 ttacattcgt aaaccatttg caatgcccct tcgcgaaggt atgagcgtat agttccttgt
     4501 tctggctgta tattgccttc taccagggca agatattttt tatcgttttt atgccagttg
     4561 tctttgataa tttccatggc tgcccgggat ttggcaaata ttagcacccc tgaagcctct
     4621 ttgtcaaggc ggtgaaccaa aaataccttg tttgcgccct tttcggcttt aattaatgat
     4681 gatatcagcc tgaatataca tggttggttg tcggtggcta cggttgctat tccggctggt
     4741 ttgtcaatgg caatgatatg ctggtcttca taaagaatat cgaaaggtat ggatttactg
     4801 gtgcttgagc catcggtaac ctgtacaagg tccccttcgc gtagcttatg agcgtatagc
     4861 cctgctggat ggtcatttac cataacccgt ccattctcaa tccatttttt tagttttgtt
     4921 ttagaggttt gtggatattc actacccaga aagtcaatta atgtgcatgg ttgctttacc
     4981 gtatgttttt gcatattcta tttttattca tttttctgtt gagtggccga agtagctgcc
     5041 agtattgctg ttgaccatgc tatttgcagg ttatacccgc cggtatcggc atcaaggtca
     5101 ataatttcac ctgcaaaata aaggttttct atcaattttg actgcatggt ctttgggtca
     5161 atttcttttg tacttatgcc acctgcagta attattgctt cggtaaatgg gcgataacca
     5221 gtgatttcaa attcgaagtt tttaagcaaa tgaataattg cttgtctttc tttagcgcta
     5281 atttggctgc aaggtttgaa tggttctatt cccagccggt taatgaaaaa tgaaattaat
     5341 tgctgaggaa gcagctcttt gaaagctatt ccaatgggtt tgcggcctaa agtattgaaa
     5401 tcacgaagaa gccgggcttc aagttgtgct tcgtccagtg ctggtttgag atcaatgtta
     5461 atgcttattt tattagcttt attttgtagc ttttctacta caaacctact caaactcagt
     5521 attattgggc catcgagccc aaaagtttca aacaacattt cgccaaagag cgattgctgt
     5581 tttttgttgt tgagtaatac tgtggctgtt acatttctga ggttgagccc ttctagttcc
     5641 tttacatttt tgtcagatac ttcaagggct acaagcgatg gcagaggctt tacaatggtg
     5701 tgcccaagtt ctccggctat tttgtaacca tcgccagatg aacctgtagc cggatatgag
     5761 caacctccgg tacaaagtat gactctgtct gaaataactg tttttgtttg attgctgtgg
     5821 atataatcaa taccagtagc ctttttattt gcagtattaa cctgagtaac ccgggctcct
     5881 tgaataatct gaacattata tcgcttaacg tattttacca aggcatctac aacatcgcgg
     5941 gctgagttac ttttaggaaa tacccgccct ccacgttcaa ttactgttgg aactccaaat
     6001 ttattgagta aatcgataat gtctgatgca aaaaatacgt tgaaagcctg acgcacaaaa
     6061 tctgggttag gataaatatg ttccagaaat gcttcaatag gggcaatatt ggttatgtta
     6121 catcggcctt tgcctgttat cagcagtttt cggcctggcc tttccatttt ttcaataatc
     6181 agcacctttg cccccaactc tgcagctctg ccagctgcca ataagcctgc ggcacctcca
     6241 ccaattacta taatatcata cttttccatt attggcccga attgaattgt ggttcttgct
     6301 tatttataag gtttatcaat gctttaactt ttgtaataca ttcatcatat tcgttttcag
     6361 gaatagattg tgctgtaatg ccacttcctg cagggattga aagatgttta tttgtgctat
     6421 tatataaaat actgcggatg acaacattga agtcgaaatt catatcaggg tcaatatatc
     6481 caatagctcc tgaatataaa ccgcgattga atgcttcaaa ttcgtcagaa tacttcacag
     6541 cattgaattt aggagcacct gtcatagaac ccattgggaa tactgcgttt ataatatcat
     6601 tgaaagatgt ttcatccttt acattacatg aaatggtgct tataagttga tgcacatgct
     6661 caaaggagta gcattggcac aactcatcaa cttttacact ttttttttct gcaaatacag
     6721 ccatgtcgtt tcgtaccaaa tctgttatca taatgttttc agcacgttct ttatcatttg
     6781 ccaataattc ttcttttatc tcgccatctt tggtaggatt atcagcacga gcggctgttc
     6841 ctttaatagg ttgagagatt attttcttac catttttctg gataaacctt tcgggcgagg
     6901 ccgaaataca aaacttgtct ttctgcttgt agaatgcgct aaatggggca ggagatattt
     6961 gtgtaagctc taaatataag ttgtatgggt caatacaagc gtttgtagca tgaaaatcta
     7021 tacaataatt gagctcgtaa acattgcctc gtgctatgtg tttttgcaat tgatgtatga
     7081 cgtttatgta ttctgatttt tttataaaat caatctctat atgagaatat gtttcttttt
     7141 tttttattaa agtaatagag gtaagagtgt ctataacgta ctttaattga tttatttctg
     7201 tttctggaga gtattgcaat tcaacttccc ttccgcaaag ttttatcaaa aatcggggtt
     7261 tgaaaaagta aaaattttga aacccaatat gattaatatt atcattaatg gtatatccta
     7321 tggtttgatt tttataatta tatgatatat ggcctaaaat ccaattttta tttatgcttt
     7381 caataaatga taaatcgctg tattgcgctg aatgcgaata gtcttcgacg cttccaattc
     7441 cggcaagcat gtcaaaacgt ccaaaaggga aaaaattttt tttgtccggg caagaatcta
     7501 agagcagaaa agtttcatat ttctcagcta attcaataag tttagcccta aaagtatata
     7561 tttggtcaat gttgaatttt attacttttt gcatggtaca aaaataggat gtttttgtaa
     7621 tttcgcagcg aaagctacat tgttaattac ttttcaaaat atgcaatagt caaaaattca
     7681 atgtgttgca gtgacgattt gtataaaaat gaaattcgca aaaagaaaaa cttgtttatt
     7741 tcacttttat tcaattactt tgcactccgt tttcaaaaat aattataaaa aagaacaaca
     7801 atgtcgaaga tttgtcaaat aacaggaaaa acctcgatga taggcaataa cgtttcacac
     7861 tcgaaacgca gaactaaaag atcttttgat atcaatttat ttacaaagaa gttttacctt
     7921 gaagaagaaa aacgttggat cgtgcttagg gtttcagctg ccggaatgag ggttatcaac
     7981 aagaatgggc ttaaagctgc tttagattca gctgtagaaa aaggtattat cttaagttat
     8041 taatattatt aaagcatata agcaatggct aaaaaaggta acagaataca agtgattttg
     8101 gaatgtaccg agcacaaaga gagcggaatg cctggcactt caaggtacat tactaccaaa
     8161 aacaagaaga atactcctga aagattggag ctcaaaaagt acaaccctat tttaaagaaa
     8221 gtaacagttc acaaagaaat aaaataagaa gactatggca aagaaagtag ttgcaacact
     8281 gaaaaccggc cttgggaaga actttaccag ggttatcaaa atggtaaaaa atcctgctac
     8341 cggatcttat ggtttcaagg aagatgttat gcacaacgaa gaaattaagg attatcttgc
     8401 aaaataattt gcggtaagac tttataaata atcgcttaca aaaaggcttt cttatacaag
     8461 agagcttttt ttgtatcatg tcgtttaggt ttttcttcat cattgatttt aacaatatta
     8521 gtacatttgt gttttgaaaa aaatagttga ttatggggct ttttgggact aaaaaaaata
     8581 atgatacgct gagttcgggc ttaaatgcta cccgacaaag cttactttca aaactatcaa
     8641 gggctattgt tggaaaatca aaggttgatg acgaagtctt agataatctt gaagaagtgc
     8701 ttatttcttc tgatgttgga gtagaaacaa ccatcagggt tattgaaaga atagaaaagc
     8761 gtgtagctaa ggataaattt gtgggcatta gcgagttaga tactattatg aaagaagaaa
     8821 tagcctcact tctttcggat aaccaaaacg atgttgctga taatttcttt gagtctgaac
     8881 ttcccaaacc ttatgtaata cttgttgttg gtgttaatgg tgttggaaaa actacaacca
     8941 ttggtaagct ggccaatagg tttaaaaaag ccgggaaaac tgttattttg ggtgctgctg
     9001 atacatttag ggctgccgcg gttgaccagt tgactatctg gtctgaaaga gtaggagtgg
     9061 agattgtaaa acagggaatg ggagcagatc cggcctctgt tgcttatgat actgttgcag
     9121 ctgcaattgc ccgtaatgtt gatgtagcaa taattgatac tgccggccgt ttgcacaata
     9181 aggttaacct catgaacgag cttaccaaga taaagaacgt tatgcgaaag ttatgcccgg
     9241 atgcaccgca cgaagtgttg ctgatattgg atgcttctac cgggcaaaat gcttttgaac
     9301 aggccaaaca atttacttcg gctacagaag ttaatgcttt ggctgtaact aagcttgatg
     9361 gaactgctaa aggtggtgtt gtgcttggta tttgtgaaca ctttaaagtg cctgtaaaat
     9421 atatcggtgt tggtgaaaaa attgatgatt tgcaactctt tgataaagtg gagtttgttg
     9481 aaaggctttt tgataaaaaa ggttaaaaca aggtgattaa tttacttcat aatattactc
     9541 aaacagccat gaaggctggt aggagacctg gcgttatcaa atacaaaaaa tacaccggct
     9601 atagtgcggc gcgtcactat cgcgacttgc ttacgcaagc aaaacatgag ataaaaaaca
     9661 gcattttgat tgtatttggc attttatctg ccggctttgg attggaaggt tttcttatcc
     9721 ccaattcttt tattgatggt ggcgtaacag ggatttcgtt gttgttttcc aaaataacca
     9781 acttacctct ttctcttttt cttatcatta ttaacatccc gtttgttata gttgggtatt
     9841 atcagattgg aaagcaattt gcgtttaaat cggcagctgc catcgttggg ctttcggtat
     9901 gcgttatttt tgtccattat ccggtcatta cctccgataa acttttgatt tcggtttttg
     9961 gcggcttctt tcttggggct ggtataggtt tggctatcag aggcgggggg gttcttgatg
    10021 gcactgaaat attatcgttg tatttgagta aaaaaattgg gctgtctata ggggacataa
    10081 ttctgataat taacattttg atatttagtg ttgcagctta tttattgggt attgaatcgg
    10141 ctttgtattc tgtacttatt tatttgagcg cttcaaaaac agttgatttc ttggttgatg
    10201 gtattgatga a
//
LOCUS       DAOSPF010000095        10045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_6700_length_10045_cov_8.887487, whole genome shotgun sequence.
ACCESSION   DAOSPF010000095 DAOSPF010000000
VERSION     DAOSPF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6700_length_10045_cov_8.887487"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="rsgA"
                     /locus_tag="PK252_13330"
     CDS             complement(<1..387)
                     /gene="rsgA"
                     /locus_tag="PK252_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HOT15735.1"
                     /translation="MEKGLVVKVTGNVCNILTDGNYLIEGRIKGKLRTKDFKATNPVA
                     VGDVVQYQRALEDEAVRISDILPRKNYIIRKSVNLSKQYHIIAANVDQAVLVVTLLQP
                     EVSVEFIDRYLVAAEAYKIPVVLVFNK"
     gene            complement(392..1345)
                     /locus_tag="PK252_13335"
     CDS             complement(392..1345)
                     /locus_tag="PK252_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT15736.1"
                     /translation="MVSNILFEQFYTYIIFMIIGYLATKTKIITLDNGVFLSHFVLYI
                     TFPLLIFTTVTHMQFDFNVIKNSIFVFLFSYISLFLLFAVGKLTSRWLCLQEASEKIH
                     ILHSMFGNIVFIGFPFISSLMPGSHALLYAAVFQVAADSVLWTLGIYLIANKKNNNFK
                     ENLKHLVNVNSVVFFVSLIMLGFGLKLPETFNKPFSMLGHTTLSLSLVFVGHILSTIK
                     IDAMVKKASLYALSFNKLILVPFIMLGIIYVLKPYLPFLQKDAVAALVLQVGMPAMAT
                     IVILARKYDADHYLATENMFVTTIFSIFTLPLLWGVINYFF"
     gene            complement(1335..2180)
                     /locus_tag="PK252_13340"
     CDS             complement(1335..2180)
                     /locus_tag="PK252_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT15737.1"
                     /translation="MTKLDDKIVQRFYLHNAVVKENKSGILTIEAGTIVYEVFRTVSG
                     IALFIEDHFERLAHSVKSVNPIIDIDNLLGLFYSDIQKLIADNDFAEGNIKIVMAFNE
                     NCVNRYVYYLPHVYPTTQMYDKGVHMVCLNAVRKNPDIKMLHQSIKEQVNKILSDSNI
                     YEVALVNENRITEGSRSNLFFIKGNIVYTAPDNEVLLGITRKYVLKILYDNNIDVVFD
                     CISLNSLPEFDAAFISGTSPKVLPVKSIENYTFSLPNSLLSFISVEYDKLMTDYINKN
                     KLYGK"
     gene            complement(2428..3675)
                     /locus_tag="PK252_13345"
     CDS             complement(2428..3675)
                     /locus_tag="PK252_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOT15738.1"
                     /translation="MKRSEIEIMAPVGSYESLMAAIQGKADAVYFGIGKLNMRSKSTL
                     NFTADDLKNIVEICHANNMKAYITLNTVMYDEDIAEMRQVLDYCKANNVDAVIASDLA
                     ALLYAKEIGLDVHISTQQNISNAEAVNFFAQYADVMVMARELDLKQVKAISRSITDNK
                     IQGPGGKPIRLEMFAHGALCMAISGKCYLSLHEYNHSANRGACFQICRRSYTVTDNET
                     KAQLEIDNEYIMSPKDLCTIGFIDKIIDAGVKVLKIEGRARSAEYVKTVCYAYHKALL
                     ALENGTYDKALIDELTAELSTVYNRGFWDGYYLGRRLGEWSESYGSKATKKKVYVGKV
                     TNYFSKLSVAEILMETNEVSIGDELLIMGPSTGVIEITASEIRVELKNAEKAVKGQYF
                     SMLVPNVVRRSDKLYKLVANEEL"
     gene            complement(3672..4811)
                     /locus_tag="PK252_13350"
     CDS             complement(3672..4811)
                     /locus_tag="PK252_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT15739.1"
                     /translation="MKILLLLLASVIGIGSLYYTHILVEELKIEEQKKIEIWAEATKQ
                     LGSSDMTDKNFDFLLKVIQNNQTVPVILTDSADNIISYRNFDSIKSLDTSYLKAQLKM
                     IKLKNERIVIDVGKQNKNYIYYKDSYILEQLSVYPYLQLGVILLFILVAYFAFSSSRK
                     AEQNQVWVGLTKETAHQLGTPLSSLMAWYELLKEETPDNPHVHEMFKDIKRLESIIER
                     FSKIGSNSPLQNENLCNVVENTVNYLRSRVSKNVTIDIDCPDRDIEVPLIRSLFEWVI
                     ENLCKNAVDAMAGSGSIQIVILERKNKIQIDVIDTGKGIQKSKFKTIFKPGYTTKKRG
                     WGLGLSLVKRIVEQYHRGKIFVYNSQIGKGTDIRILLPKTKEQGS"
     gene            complement(4841..7321)
                     /locus_tag="PK252_13355"
     CDS             complement(4841..7321)
                     /locus_tag="PK252_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOT15740.1"
                     /translation="MLLKFLKIFKYLLITIVILIFLGIAAVFILYSVKKEHFKQIIIE
                     DINQKINAALVTSSVDFTIFHDFPNVTIKLSDVKLILKGSPSDTIANIKEAAISFDIV
                     QLWHQKLKIQSVTLNTGKLLYSEATKNYIDYFNHDTSSKSMYISINYIGLKYINLTYN
                     LDKTYFNANIEQYKLRIKTKNTASYYDNKLKLSQLYYRNNAENFNLHQKIAADFQIVA
                     QPEKCELKGLSLFYGKQKLNIKGTYYIKTKNYEVSMQTRKLRILDIKGYLPKITDWAF
                     NDGAMDVSLVLNGNIDNKNALLIIGKIPVTNGKVSFKNIDFSEINTELGFKLMPFSRK
                     FDIAVNNFQCKTKGSSLQCSGIVSLANKLALHFEGNTTIKVEEFFNLLKLKDINNFTG
                     TITSGFIVDYVGKDSIFNNKNLDLTLDSKLENIKLDYQKRQFAFSGHVLLKEDRATLK
                     HGRLCFDNCSMQLNGMLDNVYKSNQTRVFKGDLAIDTLDLNNAFFSADTAGSGLELSI
                     NLKCRLLKYNQFRFQDLVARVESSSKRFACNGMRFDIWDGKIDNASYSYNENTKSAYF
                     TGNFQNLSLKSLFYNFDNFGLKYLQANNIKGSVSGKASFNCKFDKNDSIINSSISSEA
                     DMGFSQTSISDFKPLMDVFAFLKIPRPANNEVVFQPFRCQLAIANNLFTVHPVLIKNN
                     IVDFKLEGVHYLDNKFKYHLQFSLSELIKKNIRLKEDKDLPVAEDEKNDRKMMVYVLL
                     SGNDSIFNIKYDNQKAFQSFKEKLVEEKNAIKQIFQGKEIDKKPAEEKKRKIGIEENP
                     LTNEEKKTPTVKPVVKKDSKPKVEWKDE"
     gene            7368..7820
                     /gene="dtd"
                     /locus_tag="PK252_13360"
     CDS             7368..7820
                     /gene="dtd"
                     /locus_tag="PK252_13360"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT15741.1"
                     /translation="MRVVIQRVKMAEVVIDGNEKRSIKGGLLVLCGFEDADTDEDLDW
                     IAGKISRLRIFNDEAGIMNKSVEEINGQIMLISQFTLFASTKKGNRPSYSYAAKPENA
                     MPLYQRFIDIMQNYVNKDIVTGKFGADMQITLVNDGPVTIIIDSKKKE"
     gene            7817..8161
                     /locus_tag="PK252_13365"
     CDS             7817..8161
                     /locus_tag="PK252_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOT15742.1"
                     /translation="MTIQEAQHKIDGWIKQYGVRYFSELTNLAVLVEEVGELSRLMAR
                     RYGDQSVKPGEVIDDEKLAEELADVLFVLLCIANQTGTDMDKALEKSLEKKTKRDKDR
                     HITNTKLTNNGQ"
     gene            8151..9059
                     /locus_tag="PK252_13370"
     CDS             8151..9059
                     /locus_tag="PK252_13370"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOT15743.1"
                     /translation="MANSITDSSVVMQMQQTTSVPILKMPSLQEAANMQNLQEKVYVN
                     WSDHLSLKNNIFTGKYRKIALWQDTTSVKTNINIQPKVNTHISEKYDYSYFFVIAVLL
                     LLFMAYIKTTFGKHFNLFIRALFFKVDANKLYQEQSAFVNSMLWILNLNYIVTAFFGL
                     LFFFYSNNPIFFKENFSYVLGWSVAIPIILFFYRYLSVKISGWLFRMQQAFDEYNFYA
                     LLYFKYIGIFIFIILLVLLVIDQKWYPVLYIFSLIVILLTYFLLVFKGIKILLKKGVL
                     FFYLMLYLCTIEWLPILLVYKYLKSL"
     gene            9091..9849
                     /locus_tag="PK252_13375"
     CDS             9091..9849
                     /locus_tag="PK252_13375"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT15744.1"
                     /translation="MKIKKILVTQPEPDHEKSPFHDLAVKNSLKIDFRAFSHVEGLKA
                     KEFRQSRIDILEHTAIVFSAKTAIDHFFRLCDEMRITIPDSMKYFCTSESVAFYLQKY
                     IVYRKRKIFYGNNSLQDIEDVLIKNKEEKFLIPLSDTHKNDIPTLLDKLKIKYTKAVI
                     YKTVCSDLSDLANIDYDLLVFYSPASIKSLFTNFPNFQQNDIKIATFGESTAKAVKEA
                     GLRLDVHAPAPQFPSMTMALEHYIKEFNKNCKAK"
BASE COUNT         3240 a         1916 c         1681 g         3208 t
ORIGIN      
        1 tttgttaaaa accaatacca ccgggatttt gtaggcctca gcagcaacaa ggtacctgtc
       61 aatgaactct acactaactt caggctgcaa aagcgtaaca accaaaacag cctggtcaac
      121 attggctgca ataatatgat attgcttaga caagttgact gatttgcgga tgatataatt
      181 ttttctcggc aaaatgtcac taatacggac agcttcatcc tctaaagccc gttggtattg
      241 aacaacatcc ccaacagcaa ccgggtttgt agctttaaaa tctttggtac gaagtttccc
      301 tttaatgcgc ccttcaatca gataatttcc atctgtcaga atattgcaaa cattacctgt
      361 aactttaact actaaaccct tttccacaac atcagaaaaa ataattgatt acaccccata
      421 acaaaggtaa agtaaaaata ctgaatatgg tagtgacaaa catattttca gtagccaaat
      481 aatgatcggc atcatatttt cgggcaagga tgacgattgt agccatagct ggcatcccca
      541 cctgcaaaac caaagctgcg acagcatctt tttgcaaaaa aggaagatag ggtttcagga
      601 cataaattat ccctaacata ataaaaggta ctaaaattaa tttattgaaa gataaagcat
      661 acaaacttgc ctttttcacc atagcatcta ttttgatagt actcaaaata tgccctacaa
      721 aaacaaggct taacgaaagt gttgtatgcc cgagcataga aaaaggtttg ttaaacgttt
      781 caggtaattt cagcccaaag ccaagcatta ttaatgaaac aaaaaatact acagagttga
      841 cattgaccag atgttttagg ttttctttga aattattatt ttttttgttg gcaatcaggt
      901 aaatacctaa agtccacaac actgagtcgg ctgcaacctg aaacaccgcc gcataaagca
      961 aagcatggct cccgggcatg agcgaactga taaatgggaa accaatgaaa acaatgtttc
     1021 caaacatgga atgtaatata tggatttttt ctgaggcctc ttgaagacac agccacctgc
     1081 tggtcaactt tcctactgca aaaagcaaaa acaacgaaat gtatgaaaat aaaaaaacaa
     1141 aaatggagtt ttttattaca ttaaaatcaa actgcatgtg ggtaaccgta gtaaaaatca
     1201 acaaaggaaa tgttatatat aaaacaaaat gagacaaaaa aacaccatta tctaaagtaa
     1261 taattttagt tttggtagcc aaataaccaa tgatcatgaa aataatgtag gtataaaact
     1321 gttcgaaaag aatattactt accataaagc ttatttttat tgatataatc tgtcatcaat
     1381 ttatcgtatt caactgaaat aaaagacaat aaagagtttg gtaaagaaaa tgtataattt
     1441 tcaatagact tcacgggcaa tacctttgga gaagtaccag aaataaaagc agcatcaaac
     1501 tcaggtaaag aatttagcga aatacaatcg aaaacaacat ctatgttgtt atcatacagt
     1561 attttcaata catactttcg ggtaatgccc aaaagcactt cattatcagg agctgtataa
     1621 acaatattgc ctttgataaa aaacaaattg gaacggcttc cttcggtaat cctgttttcg
     1681 ttgaccaaag ctacttcata tatattactg tctgacaata ttttatttac ttgttcttta
     1741 attgattgat gaagcatttt aatgtcagga tttttcctta cagcattgag gcaaaccatg
     1801 tgaaccccct tgtcatacat ttgtgttgta ggatacacgt gagggagata ataaacatag
     1861 cggtttacac aattttcatt aaaagccatg acaattttaa tattgccttc tgcaaaatca
     1921 ttgtcagcaa taagtttttg aatatctgaa taaaatagcc ctaataaatt atcaatatct
     1981 atgattggat ttactgattt taccgaatgg gcaagccttt caaaatggtc ttcaataaaa
     2041 agagcaatgc ctgaaaccgt tctgaaaact tcatacacaa tagtaccggc ctcaatagtc
     2101 aatataccgc ttttattctc tttcaccaca gcattgtgca aataaaaacg ctgaactatt
     2161 ttatcatcca acttggtcat aactcaattt attattgaca gaaaacatat tttatataaa
     2221 ctaaaatttt aaatatttta aatacatttt caggacaacg aaattaatga ataaacagga
     2281 atatgaggca aaagtttagt aaatggggca aaaaaccgta tttataaagt cttttgatag
     2341 ttctacgccc aatgctatta actggcataa ctttcaagcc ataaacgcca agcatacaat
     2401 atatgatttt atgtttggcc acaccaatta cagctcttca ttcgccacca acttgtacag
     2461 cttatccgaa cgccttacca cattgggtac cagcattgaa aaatattgac cttttacagc
     2521 tttttcagca ttttttaatt ctacacgtat ttcagatgct gttatttcga ttactcctgt
     2581 tgatggcccc atgataagca gttcgtctcc aatagatact tcattggttt ccatcaatat
     2641 ctctgctaca cttagttttg aaaagtagtt ggttaccttt cctacataaa ccttcttttt
     2701 tgtagcttta gagccataag attcgctcca ttcgcccagc cgacggccaa ggtaatatcc
     2761 atcccaaaaa ccacggttat aaacagtact caattcagca gttagctcat caatcaatgc
     2821 tttgtcataa gtgccattct ctaatgccag cagcgcctta tgataagcat agcaaaccgt
     2881 tttcacatat tcggctgagc gggcacgtcc ttctatctta agcaccttta ccccggcatc
     2941 gatgatttta tcaataaaac caatggtgca caaatctttg ggcgacataa tgtactcatt
     3001 gtctatctca agctgcgcct tggtttcatt atcagttaca gtatatgagc gcctgcaaat
     3061 ctggaagcag gccccacgat tggccgaatg gttgtattca tgaaggctta aataacattt
     3121 gcccgaaatg gccatgcata atgccccatg agcaaacatc tccaaccgaa taggtttccc
     3181 accggggcct tgaattttat tatctgtaat agaccggctg atggctttca cttgcttgag
     3241 gtccagctcg cgggccatca ccatgacatc ggcatattgt gcaaaaaaat tcaccgcctc
     3301 agcattgctg atattttgct gagtcgaaat gtgtacatca agtcctatct cttttgcata
     3361 caacaacgca gccaagtctg aagctataac tgcatctaca ttattggctt tgcagtaatc
     3421 aagcacttgt cgcatctctg caatatcttc gtcatacatc accgtattca aagtgatata
     3481 agctttcatg ttatttgcgt ggcaaatttc aacaatgttt ttcaagtcat ctgcagtaaa
     3541 gttcagcgtt gacttggacc gcatattgag cttcccaatg ccaaaataca ccgcatcagc
     3601 ctttccctga atagcagcca tcaatgactc atacgagccc actggtgcca tgatttctat
     3661 ttcgctcctc ttcatgaacc ctgttccttt gtttttggca aaagtattct tatatcggtt
     3721 cctttgccaa tttgtgaatt gtaaacaaag attttgcccc ggtgatattg ctcaacaatt
     3781 cgtttgacca atgacaaccc aagtccccaa ccacgttttt tggtggtata accgggttta
     3841 aagatggttt taaacttcga cttttgaata cctttgcccg tatcgataac atcaatctgg
     3901 attttatttt tcctttcaag aataacaatc tggatgctgc cactgccagc catagcatcc
     3961 acagcatttt tgcacaggtt ttcaatgacc cattcgaaaa ggctccggat taaagggact
     4021 tctatatccc ggtcgggaca atcaatatca atggtaacat tttttgacac cctgctacgc
     4081 aaatagttga cagtattttc aacaacattg cacaaattct cgttttgcaa aggcgaattg
     4141 gaacctatct ttgaaaatcg ctcgatgata gactccagcc gcttgatgtc tttgaacatt
     4201 tcatgaacat gcggattgtc cggagtttct tctttaagca attcgtacca agccatcagc
     4261 gaagacaaag gagttcccaa ctgatgggct gtttcttttg tcaaccctac ccaaacctgg
     4321 ttttgttcag cctttcgtga tgagctaaat gcaaaataag caactaatat aaataacagg
     4381 atgaccccca gctgcaaata aggatataca gataattgct caagtatata tgaatctttg
     4441 taataaatgt aatttttgtt ttgctttcca acatcaataa caatccgctc atttttgagc
     4501 tttatcatct tcaactgagc cttcagatat gaagtatcta atgatttgat tgaatcaaag
     4561 tttctgtaag agatgatgtt atctgctgag tcagtcaaaa taaccggcac cgtttggttg
     4621 ttttgaatga ccttgagcaa gaaatcaaag tttttatccg tcatatctga cgaaccaagc
     4681 tgcttggtgg cttcagccca gatttcaatt ttcttctgct cctcaatttt gagctcttca
     4741 accaatatgt gagtgtaata caatgaccct atgccaatta ctgatgctaa gagcaacagt
     4801 aatattttca tccaaaattt tctattgtat atgcccataa ttattcgtct ttccattcaa
     4861 ccttaggctt cgaatccttt tttaccacag gtttaactgt tggcgttttt ttctcttcgt
     4921 tggtaagagg attttcttct atcccaattt ttcgcttttt ttcttcagca ggttttttat
     4981 ctatctcttt tccctgaaaa atttgcttga tagcattttt ttcttctacg agcttttctt
     5041 taaacgactg aaaagctttt tgattgtcat atttaatgtt aaaaatacta tcattccctg
     5101 acaataaaac atataccatc atttttcggt catttttttc atcttcagcc acgggcaaat
     5161 ctttatcctc ttttaacctg atgtttttct taatcaactc tgataaacta aattgtaaat
     5221 gatatttaaa cttattatct aaataatgta ctccttcaag cttaaaatca acaatattat
     5281 tttttatcaa aaccggatgg acggtaaaaa ggttattggc aatagctaac tggcagcgaa
     5341 atggctggaa tactacctca ttgttggccg ggcgtggtat tttaaggaag gcaaacacgt
     5401 ccatcaaagg tttgaaatcg ctgatgctgg tttgactaaa tcccatatcc gcttcagaac
     5461 tgatgctcga attgatgata ctgtcattct tatcaaattt acagttaaaa ctggcttttc
     5521 ctgaaacaga ccctttgata ttattggctt gcagatattt caacccaaaa ttatcgaaat
     5581 tataaaaaag tgatttcaac gaaaggtttt gaaaattgcc ggtaaaataa gcacttttcg
     5641 tattttcgtt gtatgaataa ctggcattat caatcttccc atcccatata tcaaaacgca
     5701 taccgttgca tgcgaacctt tttgaagaac tttcaaccct ggccaccagg tcctgaaacc
     5761 gaaactgatt atatttcagc agtctgcatt ttaggttaat acttagctct aaaccactac
     5821 cggcagtatc agcagaaaaa aaggcattgt ttaagtccag cgtatctatg gccaaatctc
     5881 ctttaaaaac acgcgtttgg tttgatttat aaacattatc cagcatcccg ttgagttgca
     5941 tagaacaatt atcaaagcaa agccggccat gtttgagtgt agccctgtct tctttcaaca
     6001 gaacatgccc cgaaaatgca aattgtcttt tttgatagtc cagtttgatg ttttcaagct
     6061 tagaatctaa ggtaaggtca agatttttat tgttaaaaat ggaatcttta cccacataat
     6121 caacaataaa accggaagta attgttccgg taaaattatt tatatctttt aattttaata
     6181 aattaaagaa ctcttctacc tttatcgttg tatttccttc aaaatgcaaa gccaatttgt
     6241 tggccaaaga aactataccc gagcattgaa gggaagagcc ttttgttttg cactgaaagt
     6301 tgttgactgc aatatcaaat tttctggaaa aaggcattag tttaaacccc aattcagtat
     6361 tgatttcaga aaaatcaata tttttaaaac ttactttgcc attggtaacc gggatttttc
     6421 caattattag taacgcattt ttgttgtcaa tattgccgtt gagcacgagc gaaacatcca
     6481 tagccccatc attaaaagcc caatcagtga tttttggcaa atatcctttt atatctaaaa
     6541 tccggagttt acgagtctgc attgaaacct cataattttt tgttttaata taatatgttc
     6601 cctttatatt caacttttgc ttaccgtaaa acaggctgag tcctttaagt tcgcattttt
     6661 caggttgagc aacgatttga aaatcggcag caattttttg atgaaggttg aaattttctg
     6721 cattatttct gtaatataac tgacttaatt ttagcttatt atcataatat gaggccgtat
     6781 tttttgtctt aattctaagt ttatattgtt ctatattagc attaaagtat gttttatcta
     6841 aattataagt taaattaata tattttaatc cgatataatt aatgctgata tacattgatt
     6901 tgctgctggt atcatggttg aaatagtcga tgtaattttt agtcgcctcg gagtatagca
     6961 gtttcccggt atttaaggtc acagattgta tctttagctt ttggtgccac agttggacaa
     7021 tatcaaaaga tatggcagct tctttaatat ttgcaatagt atcagaagga ctgcctttga
     7081 gaatcagctt tacatccgac aattttatag tcacattggg aaaatcgtga aaaatagtga
     7141 aatcaacaga tgaggtaacc agtgcggcat tgattttttg gttaatatcc tcaataatta
     7201 tttgtttaaa atgctctttc ttgaccgagt agaggataaa aaccgcagca atgccaagaa
     7261 aaatcaaaat aacgatggta atgagcaaat acttaaatat tttgagaaac ttcaaaagca
     7321 tagtattatt tttggcaaat tgtaagtgca aaggtaagca aaagaaaatg agagttgtta
     7381 ttcagcgggt gaaaatggct gaggtcgtta tagatggcaa tgaaaagagg agcatcaaag
     7441 gtggccttct tgtactttgt ggttttgaag atgccgacac cgacgaagac cttgactgga
     7501 ttgccggaaa aatatcacgg cttcgaattt tcaatgacga agcaggcata atgaacaaaa
     7561 gtgttgaaga aataaatggg caaataatgt tgataagcca gtttacgttg tttgccagca
     7621 ccaaaaaggg caacaggcca tcttattcgt atgctgccaa gcctgaaaat gccatgccgc
     7681 tatatcaacg ttttattgat atcatgcaaa actatgtaaa caaagatatt gtgacaggca
     7741 agtttggcgc cgacatgcag attaccctgg tcaacgatgg gccggtgact attattatag
     7801 attcgaaaaa gaaagaatga ccatacagga agctcaacat aagatagatg gctggattaa
     7861 gcaatacggg gtaaggtatt tctccgaatt gaccaacctg gctgtgcttg ttgaagaggt
     7921 aggcgaactt tcgcgactga tggccaggcg ttatggcgac cagtcagtta agcccggcga
     7981 agttattgat gacgaaaaac tggccgaaga actggccgat gtgctttttg ttttgctatg
     8041 tatagccaat caaaccggca ccgacatgga caaagctttg gaaaaaagcc tggaaaaaaa
     8101 aacaaaacgc gataaagaca ggcatataac aaatacaaaa ctcacaaaca atggccaata
     8161 gcattaccga ctcatctgta gtaatgcaga tgcaacaaac aacctctgta cctatcctga
     8221 aaatgccatc attgcaggaa gctgccaaca tgcaaaactt gcaggaaaaa gtttatgtca
     8281 actggagtga ccacctgtca ttgaaaaaca acatttttac cgggaaatat cgaaaaattg
     8341 cactttggca agataccaca agtgttaaaa ccaacataaa cattcaacca aaagtcaata
     8401 cccatatcag cgaaaaatat gattacagct atttttttgt catagccgtt ttattgctcc
     8461 ttttcatggc ttatattaaa accacctttg gcaaacactt caaccttttc atcagggcgc
     8521 tattttttaa agtagatgcc aataaacttt atcaggagca aagtgcattt gtcaatagta
     8581 tgctgtggat actcaaccta aattatattg tgacagcttt ttttggctta ctatttttct
     8641 tttattcaaa taacccgata tttttcaaag agaacttttc gtatgttctt ggctggagtg
     8701 tagcaattcc cataatttta ttcttttaca ggtatttgtc tgtaaaaatt tcaggttggt
     8761 tgtttcgtat gcagcaagct tttgatgaat ataactttta tgcattgctg tattttaaat
     8821 atatcggaat atttattttc attatcttat tggttttatt ggtaattgac caaaagtggt
     8881 atccagttct atatattttt agcctgatag taattttatt aacatatttt ctcttagttt
     8941 tcaaaggcat aaaaatattg ttgaaaaaag gtgttttatt tttttatttg atgttgtacc
     9001 tttgtaccat cgaatggttg ccaatattac ttgtttacaa atatctgaaa agtttgtaaa
     9061 ccaggatgtt aaactaaata atgaatagct ttgaagatta aaaaaattct agtgactcag
     9121 ccggaaccag atcatgaaaa atccccgttt catgatttag ctgtaaaaaa tagcctgaaa
     9181 attgatttta gggcatttag tcacgtcgaa gggttgaaag ccaaagaatt cagacagtcg
     9241 cgtattgata tcctcgaaca tactgcaatt gttttttctg ccaaaacggc aatagatcat
     9301 tttttcaggc tctgcgacga aatgcgcatc actatccccg atagcatgaa gtatttctgt
     9361 acttctgagt cggttgcttt ttatctgcaa aaatatattg tctatcgcaa acgcaaaata
     9421 ttttatggaa ataactctct tcaagatatt gaagatgtgc ttattaaaaa taaagaagag
     9481 aaatttttga tccctctttc agatacccat aaaaacgata tcccaacctt gttggacaag
     9541 ttgaaaatta aatataccaa agcagttata tacaaaactg tatgtagtga cctttctgac
     9601 ctggccaata ttgattatga cctgttggta ttttattcac cggcaagtat taaatctttg
     9661 ttcaccaatt ttcctaattt tcaacaaaat gatattaaga ttgccacatt tggtgagtcc
     9721 acagctaagg ctgtaaagga agccggcctg cgccttgacg ttcatgcccc tgcccctcag
     9781 ttcccttcta tgactatggc tttggaacat tacatcaagg agtttaacaa aaactgtaaa
     9841 gcaaaataaa ggataacttt ctgaaataca tagagcacgc ttaaaggcgt gcttttttgt
     9901 ttacaagtga attaaatttt aacgtcagat ataagaataa cattttcaat tattgtttaa
     9961 aaagcattga attgcaataa atactaattt tctatgcaac ttttttatcg aaaaaagttg
    10021 ctcaaaaact cttatcgcct taact
//
LOCUS       DAOSPF010000096         9646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7044_length_9646_cov_7.446147, whole genome shotgun sequence.
ACCESSION   DAOSPF010000096 DAOSPF010000000
VERSION     DAOSPF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_9646_cov_7.446147"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1075)
                     /locus_tag="PK252_13380"
     CDS             complement(2..1075)
                     /locus_tag="PK252_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15745.1"
                     /translation="MKRNFLKISLLALSSVALLLTSCEKSEDTPDITANNLKGMFVVC
                     EGNFGSANGDITFYNSDPAKTVKNLYNSANGLPVGDVVQAFEIVDTLGFIVVNNSQKV
                     TVVNMRNFKVIKNIGGFSYPRNVVRANENTVYISNGKGKNYENNYIYSIDLKTLTKSD
                     SLDVETGPEKLIVVNSKVYAAIAGGWDNDGNTVIEIDPSTFKITNTFEVASVPIDLVT
                     DKDNNIWAYCKGVADKNNYSPLTYTNSGISKINLSTKVVNNFPLKTMYASEVNCIAAN
                     KDGSIIYYLNDGLYAMAITATELPTAKLVDHMFFGMDVDPKTGNIICLDDANSMAIGY
                     NAKGEKLFSFGTASFPNSVVFSY"
     gene            complement(1110..2987)
                     /locus_tag="PK252_13385"
     CDS             complement(1110..2987)
                     /locus_tag="PK252_13385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT15746.1"
                     /translation="MPILLTIIGNDIAVAQSGNDTIIEVPDVVVTATRSQYFGNDIKT
                     EVYTRNKTTDFAGESITRLLTSNSALNVKAYGAGGGLANITLRGSSASQVQVNWNGFP
                     INSVTAGACDFSMVPASGFDRVSVVYGASGAVYGSGTFGGAVNLDNNLTPSETLSGSA
                     HIGYQSLKTIDGSIFCKVGNGKAAWQLNCWGAASKNEFKYYDYINQYQRTQTDGDWHD
                     IGTIQNAAFRLSPTSTLEVGFWYQVKAYNIPSHIGSTAYEYQKDSTLKLFAAYKLHGE
                     NWGLQVKAARFDDEQHYWQKASAQATVNSIDSRIQAVQWYGDVNFRYYITPHLSVDAG
                     SIGALISADVSAYGNTKEEKELASFVGIKYDNKQFSWQAALRKEWNSDFHSDLLPSVG
                     AAVKIIPDTWTVRANASKKFRKPTFNDRYWMPGGNINLKAENGYSAELGTLATFWKSN
                     ASRLSADISLYWSQVKDMIVWRPAGAYWEAKNYQQVHSYGAETKIMFNLQKPQWQYQS
                     ELMVTLNRALFKTEVDNQEKPMIYSPRIITTWKNQFNVGFLEFTLWHHFTADRFYDDN
                     ALLKPYQTIDAQIGIKQSLGKGKIGLLFAAYNLTNKAYELIRLYPMPGRYWSVKLNYM
                     F"
     regulatory      complement(3125..3370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4094..5461
                     /locus_tag="PK252_13390"
     CDS             4094..5461
                     /locus_tag="PK252_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOT15747.1"
                     /translation="MKQSTVPPIVKHLSESVVKASKIDPGLFEKYSVKRGLRNADHSG
                     VLVGLTNVGDVVGYQKVGDTLEPIDGKLIYRGINVEALVQGAIDQNRHGFDEVVYLLL
                     SGKLPNQQELEEFMQYMGKLRDLPDDFTNNTILANKGQNVMNMLARTVLGLYNLDKNA
                     EDTSPENLVNQSLNLIAKFPTIIAYSYHALRHFYQHKTLSIRHPNPNLSTAENFLLMM
                     KGEGKYSKLEADILDLALILHADHGGGNNSTFTIRVTSSTGTDTYSAVASAIASLKGP
                     LHGGANHEVLGMMENIKENVKHWDNPKEVSDYLVKMLNKQAYDRSGKIYGIGHAVYTI
                     SDPRAVLLKKQARELAKEKGRLEEFELYESIEHLAPQIFYEFKGDKVAKRVCANVDFY
                     SGFVYNCIGIKEPLYTPLFAMSRIAGWCAHRLEELTFDAKRIIRPAYKNVYPEQSYIQ
                     LKDRE"
     gene            complement(5763..9101)
                     /locus_tag="PK252_13395"
     CDS             complement(5763..9101)
                     /locus_tag="PK252_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15748.1"
                     /translation="MNTKPTYEELLTEIAKLKQQLEEKTTGHPLSTFQDSFVQQNTKL
                     QNEKDEYEAINEELRQTNLELSLAKEKAEKSEKIYLKLVENSPWGMHFYKLKNNALIF
                     IGANPAANKLLGVDNSQFIGKTIEEAFPPLINTEVPFRYREAAEKGILWKTEQINYQD
                     NEISGAFEVIAYQISAGKMVAVFNDITERKRYEKQLKEKNEEFEAQNEEYQQLNEELT
                     QTNEELINTKNRIEKSEERFRLMLKNSNDTFVLVNKNGEQFFISDAAARDTGFTIEEL
                     MGPIQNVIYPPDLDIVLQAWNKVLNTRNETVKLQYRHKHKHKEYVWFEAVAQNHLDNP
                     SINAVVINVRDITNIKETEAILINAKEQAEANELKIKQQAEEIENFFNCAIDLLCIAN
                     IDGHFERLNQEWQNTLGYTLDELEGKRFLDFIHPEDIPSTLEIISQLESQKVILNFTN
                     RYRCKDGSYRWIEWRSYPNGNKIYAAARDITERKKVEDELLREKTFIEAIFNCVPGML
                     YLYNEKGQIVRWNQKHELMTGYSAEEMSHMHLLDWYKDDEKSQKAVIEGVKTTMEKGF
                     GEAEAYLQKKDGTKIPMYFTASPLTIYGKNYFAGIGIDITERKRVEEELKKNEILTRT
                     AIENLPIIFYMIDNDGLFKLSIGAGLKSLGLKPNQVVGLSVFELYKDHPLIIENVKKA
                     LSGEIASFESTVNGASHFNIVTPFSISDKRDGIVGVALDITERKQIEHEAILAKEKAE
                     ENDRLKTAFLQNMSHEIRTPMNAIMGFTSLMAENYNNKEKLVQYSNIIERRCNDLLAI
                     INDILDISKIESGQSTLQIEECNINEVFTELTIFFADYQNRTNKQHINLLMHHVLDEN
                     IINIKTDKLKLKQILINLISNAFKYTETGKIECGCILDNNKLHFYVSDTGIGIPEEKH
                     KFIFDRFFRIDTVISKNYVGGTGLGLPIVKGLTNLLGGEVWFESECNKGSTFHFTIDY
                     QKSNMPNPIPVIQAETRQNFVPEKKLLIVEDDEYNVMYLQELLNRHFSKIYIAETGQK
                     AIKLVHEHAVDLVLMDVRLPDMTGYEATNEIIKHFPTLKIIAQTAYAANDERYKALEA
                     GCVDYISKPTKGEILLSIISKYLE"
     gene            9359..9598
                     /locus_tag="PK252_13400"
     CDS             9359..9598
                     /locus_tag="PK252_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15749.1"
                     /translation="MKFTREDYNRRIKDTDGKIPEDEPVFLLRAQDKYAPDTLRFYAN
                     LLKEDGNTEMAEELLAHARNMLVWQKSVRVKKPDK"
BASE COUNT         2732 a         1846 c         1851 g         3217 t
ORIGIN      
        1 attaataact aaaaactaca gaatttggga agcttgcagt gccaaagcta aatagctttt
       61 cgcctttggc gttgtaccca attgccatag aattggcatc gtctaagcaa atgatgttgc
      121 cggtttttgg gtcaacatcc atgccaaaaa acatatgatc aactagtttt gctgtaggta
      181 gttctgtagc tgtgattgcc atagcataaa gtccatcatt gagataataa attatactac
      241 catctttatt tgctgctata caatttacct cagatgcgta cattgttttt aaagggaagt
      301 tatttaccac ttttgtcgat aagtttattt tgctaatacc cgaattagta taagtcaaag
      361 gtgaatagtt atttttatcc gctactcctt tacaatatgc ccaaatatta ttgtctttat
      421 cagttaccaa gtcaattgga acagaagcaa cttcaaatgt attggtaatt ttaaaggtag
      481 acggatctat ttcaattaca gtatttccat cattatccca tccacccgca atagctgcgt
      541 aaacttttga atttactact atcaattttt caggacctgt ttcaacgtct aatgaatcgc
      601 tttttgtaag cgtctttaaa tcaatactat agatataatt attttcataa tttttccctt
      661 taccgttgga tatataaaca gtattttcat tagctctaac tacgtttcga ggataagaga
      721 atcctccaat gtttttaatt accttgaaat ttcgcatatt tactacggtc accttttgcg
      781 agttgttaac tacaataaag cctaaagtgt ccacaatttc aaatgcttgt actacgtcgc
      841 ccacaggaag tccattagca ctattataaa ggttttttac agtcttggca gggtcgctgt
      901 tgtaaaaagt aatatcgcca ttggcactgc caaagtttcc ttcgcatact acaaacatcc
      961 ctttcaggtt gttggccgtg atgtccgggg tgtcttctga tttttcgcaa gaagtcaaca
     1021 ataaagctac actgctaagt gctaaaagtg agattttcaa aaagttacgt ttcataatat
     1081 taaaagttta taatgattaa taatactttt caaaacatat aattcaattt tacagaccaa
     1141 tacctccccg gcattggata aagcctgatg agctcataag ctttgttggt caggttataa
     1201 gcggcgaaca gcagtcctat ttttcctttg cctaaggatt gctttattcc aatttgtgca
     1261 tctatcgttt gataaggctt tagtaaagca ttatcgtcat aaaaacggtc ggctgtaaag
     1321 tggtgccaca aagtgaattc cagaaaccca acattgaact ggtttttcca ggtggtgata
     1381 atcctgggcg agtaaatcat tggcttttcc tggttgtcaa cttcagtctt gaataacgcc
     1441 ctgtttaaag taaccatcag ctctgattgg tattgccatt gcggtttctg gaggttaaac
     1501 ataattttgg tctcagctcc ataagaatgc acttgctggt agtttttggc ttcccaataa
     1561 gccccggcag ggcgccagac aatcatatct ttcacttgcg accaatacag gctaatgtct
     1621 gccgacaatc tggaagcatt acttttccaa aaggtagcta atgtgcccaa ttctgccgaa
     1681 tagccatttt cggctttcag gttgatattg ccgccaggca tccagtaacg gtcgttgaac
     1741 gtgggtttgc ggaacttctt agaagcattt gccctgacag tccatgtgtc ggggattatt
     1801 ttaaccgctg cacccaccga aggcaacaaa tccgaatgaa agtcgctgtt ccattctttg
     1861 cgaagcgctg cttgccacga aaactgttta ttgtcatatt taatgccgac aaaagaagcc
     1921 agttcctttt cttctttggt gtttccatag gctgaaacgt ctgcagagat aagagccccg
     1981 atagaacctg catcaacaga caaatgcggt gttatgtaat atctaaaatt aacatcgcca
     2041 taccattgaa cggcttggat gcgcgaatca atgctgttga ctgtggcttg tgccgaagct
     2101 ttttgccaat aatgttgttc gtcgtcaaaa cgggcagctt tcacttgcaa tccccagttt
     2161 tcgccatgca gtttgtaggc tgcaaaaagc tttaaagtgg agtctttctg gtattcataa
     2221 gctgtagaac ctatatgcga agggatgtta tacgctttaa cctggtacca aaagccaact
     2281 tcaagggttg atgttggcga aagcctgaac gcagcgtttt gtatcgttcc tatatcgtgc
     2341 cagtcgccat cggtctgggt tcgctgatac tggttgatgt aatcgtaata tttgaattca
     2401 tttttagaag ctgcacccca acaattcaat tgccaagcag ctttgccatt tcctactttg
     2461 cagaaaatgc ttccatcaat ggttttcaat gattgatagc ctatatgagc tgacccgctt
     2521 agtgtttcgg aaggcgttaa attgttgtcg agatttacag caccaccaaa agtgccgctt
     2581 ccgtaaacag ctcctgaagc tccatatact actgatacac ggtcaaatcc gctggccgga
     2641 accatagaaa agtcgcaagc cccggctgta actgagttga taggaaaacc gttccagttg
     2701 acttgtacct ggctggcaga tgagcccctc agggtaatgt tggcaaggcc tccgcctgca
     2761 ccgtaagctt taacattaag cgctgagttg cttgtcaata aacgggtaat ggattcgcca
     2821 gcaaaatcag tagttttatt tctggtgtaa acctctgttt taatatcgtt tccaaaatac
     2881 tggctgcggg tagcagtaac aacaacatcg gggacttcga taatagtgtc gttccctgat
     2941 tgagctacag ctatgtcatt gccaataatc gtaagcaata ttggcaagcc aataatttgg
     3001 gaaaagaata aagcctttaa atgcattgtt accaaataat tttaatcaaa actattcgta
     3061 actttaacaa aaacaagagg atagatacac caaggatgtc ctattctttg cttttctccc
     3121 gaaagctacg aattacattg caggcaggtc ttctggcttt cccgattttc aacaccttcc
     3181 cgctatgcat aggcataaca gtggtttttt ggttgaaaat acaataccga aaatcggtat
     3241 cgggattaca gctacgggga tagctcctga cttcctgctt tcgcattgct acggcagcag
     3301 gtcacaggat tcccatttaa tcctttgttc cgactgaacc ggaaccggaa ccagcaattt
     3361 tttgcaaatg aagaaaattt tttttaaatt aaaaacattg atgcctgaaa aaatatgaaa
     3421 cacactgact ttctttattt tatcaggttc ttaaaaattt agaaagaact gtttcatttt
     3481 aagccaaatt taatattaga atatatttaa tcgattaata agttgtaatt cagtaattta
     3541 ataaatcgca ttaaacattt tcaaaatcgg cttgtccgtt tataacgaga ggtttaaccc
     3601 aactaaatct atagttcggc aaaatattac ctcctaatgc actgtcgttt agtttagact
     3661 ctgacaggtg ctgcgaacgg tgtcaggtca gatgtgaaac taacccgaaa gcgtctttct
     3721 gacctgtcgg cgtatttttt aagtacttaa ctaaacgaca tggtctccta atgattaaat
     3781 tgggataaag gcttcatttt gtaacaatat tgactttttg ggtacaaaaa actcaaccaa
     3841 aaacttttct cctttgtgtt tctcgggatt tgttccaaaa taaggtttgt catctggcgc
     3901 atagccactt ttacgtagcc attgaccata caccattcac atgctgcttg aaaatcctgt
     3961 gttttttctg aagatttttt atttatttga gcaaaggcgt tttttggatt atctttggtg
     4021 tagttattgt tgaagattaa acaaatagat atttcaggcg ttataataaa aataacattc
     4081 aaaacaaagt aatatgaagc aatcaacagt gccaccaatt gtaaaacacc tttcggaatc
     4141 ggtagtaaaa gcctcaaaaa ttgacccagg cttatttgaa aaatatagtg taaaacgagg
     4201 gcttcgcaat gccgaccatt cgggggtatt ggtagggctt actaatgttg gggacgttgt
     4261 tggttaccaa aaagttggtg atacactcga acctattgat ggaaagctta tctatcgagg
     4321 tatcaatgtt gaagcattgg ttcagggcgc tatagaccaa aaccgtcacg gatttgacga
     4381 ggttgtatat ttgctccttt cggggaagtt gcccaaccaa caagaattgg aagagtttat
     4441 gcaatatatg ggcaaactgc gcgacctgcc cgacgacttt accaataaca ccatcttggc
     4501 caacaaaggc caaaacgtaa tgaacatgtt ggctcgtact gtattgggtt tgtacaatct
     4561 ggataaaaat gcagaagata cttcgccaga aaacctggta aaccagtcgc ttaacctgat
     4621 agccaaattt cctaccatta ttgcttattc gtatcatgct ttgcggcatt tttatcagca
     4681 caagacactt tctatacgtc acccaaatcc aaacctcagc actgcagaaa actttttgct
     4741 gatgatgaaa ggagaaggga aatattcaaa gcttgaagct gatatacttg accttgcttt
     4801 gatattgcat gccgaccatg gcggaggaaa caactcaact tttactattc gtgtaaccag
     4861 ctcaacaggg accgatactt actcggcagt agcttctgcc atagcttcat tgaaggggcc
     4921 attgcacgga ggcgcaaacc acgaagtatt gggcatgatg gaaaacatta aagagaatgt
     4981 taaacattgg gataacccga aagaagtttc tgattacctt gtaaaaatgc ttaataaaca
     5041 agcatacgac cgttcgggta agatttatgg tatcggccat gcagtatata ccatctctga
     5101 ccctcgtgct gtattgttga aaaaacaagc tcgcgagctg gctaaagaaa aaggccgttt
     5161 ggaagagttt gagctttatg agtcgataga acatcttgcg cctcaaattt tctatgaatt
     5221 taagggcgac aaagtggcta agcgagtgtg tgccaatgtt gacttttact caggctttgt
     5281 atataactgt attggcataa aagagccgtt atataccccg ttgtttgcta tgtcgcgtat
     5341 agcaggctgg tgtgctcacc gtcttgaaga gcttactttt gatgccaaac gtattatccg
     5401 tccggcttat aagaatgttt atcctgaaca aagttatatt cagctgaaag acagggaata
     5461 gtaaaatact gtttgagttt ttttaaaact ctctccaaga ggcagtgtaa aaagaaatat
     5521 taaccgcaaa gttcacgaag aaagaataaa gtacgcaaag ttttaaatgt tgattattaa
     5581 tactttgcgt gctttgcgga aaaacttagc gacctttgcg gttagctttt tttgaccttt
     5641 ttacacactc tcaaatatga taatattgtt ggcctgatga atccatctat cgttcataag
     5701 attgctcctt ttaattttga ttgggcaacc ataagtgttt ttatttggta agcttcatgt
     5761 tattattcca aatatttact tataatagat aatagtattt cgccttttgt tggtttgctg
     5821 atataatcaa cacagccggc ttcaagggct ttataacgct catcatttgc agcataagct
     5881 gtttgtgcta ttatttttag ggtcggaaaa tgttttatta tctcattagt agcttcatat
     5941 ccagtcatat cgggcaggcg aacgtccatt agcactaagt caacagcatg ctcgtgtacg
     6001 agctttatag ctttctggcc ggtttctgca atataaattt ttgaaaagtg tctatttaac
     6061 agctcttgca agtacattac attgtattcg tcatcttcta caataagtag ttttttttca
     6121 ggaacaaaat tttgtcgggt ttctgcctgt attacaggta ttggattggg catattgctt
     6181 ttttgatagt cgatggtaaa gtgaaaggtt gagcctttgt tacattctga ctcaaaccat
     6241 acttccccgc ccagtaaatt tgttaacccc ttaacaatag gtaagcctaa acctgttccg
     6301 cctacataat ttttagaaat cactgtatca atgcggaaga aacggtcaaa tataaatttg
     6361 tgtttttctt caggaatgcc aatgcctgta tcggatacat aaaaatgcaa tttgttgttg
     6421 tcaagtatgc agccgcattc aattttaccg gtttcggtat atttaaaagc atttgaaata
     6481 aggtttataa gtatttgttt tagtttaagc ttatcggttt taatattgat aatattttca
     6541 tctaatacat ggtgcatcaa caaatttata tgctgcttgt ttgtgcggtt ttgataatca
     6601 gcaaaaaata tggtaagttc tgtaaatacc tcgttaatat tgcattcttc aatttgcaat
     6661 gtactctggc ctgattcaat ttttgaaatg tcgagaatat cgttaatgat agccagcaaa
     6721 tcgttgcacc gacgttcaat aatatttgag tattgtacaa gtttttcttt gttattgtaa
     6781 ttttcggcca tcagacttgt aaaacccata atggcattca tgggtgtgcg tatttcgtgg
     6841 ctcatgtttt gcaaaaaggc tgttttcagg cggtcatttt cttcggcttt ttctttggcc
     6901 aaaatagctt catgttctat ctgtttacgt tcggtaatgt ctaatgctac accaactatt
     6961 ccatctcttt tgtctgaaat ggagaacggg gttacaatgt taaaatgcga agctccatta
     7021 acagtagatt caaaactagc tatttcgccc gaaagggctt tttttacatt ttcaattata
     7081 agcgggtgat ctttatagag ttcaaaaaca gataagccaa caacctggtt tggcttgaga
     7141 cccagacttt taaggccggc cccaatagaa agtttaaata gcccgtcatt gtcaatcata
     7201 taaaatatga tgggtaaatt ttctatggct gtccttgtca atatttcgtt tttctttagt
     7261 tcttcttcta ctcgcttgcg ttcggtgata tcaatgccaa tgccggcaaa gtagtttttg
     7321 ccataaattg tcagcgggct tgcagtaaaa tacattggga ttttggtccc gtctttcttt
     7381 tgaaggtaag cttctgcttc gccaaagcct ttttccatag tggttttaac tccttcaatc
     7441 actgcttttt ggcttttttc atcatccttg taccagtcaa gaaggtgcat atgcgacatt
     7501 tcttcagcgc tatatccggt catcaactca tgtttttggt tccagcgtac aatctgtcct
     7561 ttttcattgt acaagtatag catccccggg acacaattaa atatagcctc tataaatgtt
     7621 ttttcgcgaa gcagttcgtc ttccactttc ttacgttcgg taatatctct ggctgctgca
     7681 tatatcttat tgccgtttgg ataagatcgc cactcaatcc atctgtacga tccatctttg
     7741 caccggtacc tattagtaaa attcaggatt actttttgac tttcaagttg tgatataatt
     7801 tcaagtgtag aagggatatc ttcggggtga atgaagtcga ggaaacgttt cccttctaat
     7861 tcatcgagtg tatagccaag tgtattttgc cattcctggt tgagccgctc aaaatgccca
     7921 tcgatattgg caatgcatag caaatcaatg gcacagttga agaagttttc aatttcttca
     7981 gcctgttgtt taattttcag ttcgtttgct tcggcctgct ctttagcatt aattagtatt
     8041 gcttctgttt ctttaatatt agttatatct ctaacgttga taaccacagc atttatagaa
     8101 gggttatcga gatggttctg ggccacggct tcaaaccaga catattcttt gtgcttgtgt
     8161 ttgtgcctat attgaagctt taccgtttca tttctggtgt tcaacacttt attccatgcc
     8221 tgcaatacta tatccaaatc gggcggataa ataacatttt gaattggccc catgagttct
     8281 tcaatggtaa agcctgtatc tcttgctgcg gcatcgctga tgaagaattg ttcgccattt
     8341 ttgtttacca atacaaaagt gtcattagag tttttgagca tcagccgaaa gcgttcttcg
     8401 cttttttcaa tcctgttttt ggtatttatt agttcttcat tggtttgtgt caattcttcg
     8461 tttagttgtt ggtattcttc gttttgggct tcaaactctt cgtttttttc tttcagttgt
     8521 ttttcatatc gcttgcgttc ggtaatatcg ttgaaaactg ctaccatctt accggcgcta
     8581 atttgatagg ctattacctc aaaagctccc gaaatttcat tgtcttggta attaatttgt
     8641 tcagttttcc acaaaattcc tttttcggct gcttcgcggt acctaaatgg tacttctgtg
     8701 ttaataagcg gaggaaaagc ttcttctatg gtttttccaa tgaattgtga gttatcaacc
     8761 cctaaaagct tattggctgc cgggttagcc ccgataaaaa tcaaagcgtt gtttttgagc
     8821 ttataaaaat gcattcccca gggagaattt tctaccaact tgaggtatat cttttcgctc
     8881 ttttcggctt tttctttggc taaagataac tcaaggttgg tttgtctcag ttcttcgtta
     8941 atagcttcat attcgtcttt ttcgttttga agttttgtat tttgttggac aaatgaatct
     9001 tggaaggtag aaagggggtg tccggttgtt ttttcttcca gttgttgctt tagttttgca
     9061 atttcggtca acagttcttc gtatgttggc ttggtgttca ttccttattt tttaatacaa
     9121 ttaaatttac agaagaaaat ttacattgta aacatcttaa cccaaaaaag aaaagcttta
     9181 acccaactaa atcatttact tgggcaaata aagattaatt ttttttattt tttattgagc
     9241 ataagaaagg cttaactggc accataaaaa tgaggtcagc atttgaaaaa tattttagcc
     9301 tgacaatagt gtttttatat tagttttgta ctgttaatgg gatagtcata cttcagctat
     9361 gaaatttaca cgagaagatt ataaccggcg gataaaggat acagatggga aaatacccga
     9421 agatgagcct gtatttttat tgcgggctca ggacaaatat gctcctgaca cattgaggtt
     9481 ttatgccaat cttttaaaag aagatggaaa tactgaaatg gctgaggaac ttcttgccca
     9541 tgcccgcaat atgctggtgt ggcaaaagag cgtgagggtg aaaaaacctg ataaataatt
     9601 agcaaattag caaattagca aattagcaaa ttagcaaatt agcaaa
//
LOCUS       DAOSPF010000097         9411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7256_length_9411_cov_8.288799, whole genome shotgun sequence.
ACCESSION   DAOSPF010000097 DAOSPF010000000
VERSION     DAOSPF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7256_length_9411_cov_8.288799"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..977)
                     /locus_tag="PK252_13405"
     CDS             complement(63..977)
                     /locus_tag="PK252_13405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT15750.1"
                     /translation="MIKKIITAIAILVAALAIFLCIPSNSYLRQALLHGNVNTDDYKF
                     FKNRNVSKDQTQEWEKATGYNCKDVPKNYQDIMFRLQTHAFLVVQHGKIVHEQYFGNF
                     NDTCISNSFSVAKSIIGLLVGCALDDSLIKSLDEPVWHYLPTFNDSMGKALSIRHLLT
                     MSSGLDWDERYTSPFSPTTKAYYGNDLKKLILERKITEQPGKKFRYLSANTQLLAFIL
                     EKATGKSVSEYASEKIWRPLGASKDALWSLDNANGNEKAYCCFNAIARDFARIGQLVL
                     NKGRWNGKQIISEKYIKQRQMEWETNHF"
     gene            complement(990..1340)
                     /locus_tag="PK252_13410"
     CDS             complement(990..1340)
                     /locus_tag="PK252_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOT15751.1"
                     /translation="MDIETVRNYCNSLPGVSECFPFDEDTLVFKVDNKMFLLLSLEPP
                     FSMNVKCDPDKAISLREQYSWVLPGYHMNKTHWNTVMLEEAPNRKLTEQWIKDSYDLV
                     CASLPRKSAFYKKL"
     gene            complement(1361..1723)
                     /locus_tag="PK252_13415"
     CDS             complement(1361..1723)
                     /locus_tag="PK252_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT15752.1"
                     /translation="MTNAKTIGDKGEEIAAQYLANKGYKILSRNWQLMHRELDIVAMK
                     DQTLVFVEVKTRAEDFIVSPAEAVSRRKQKLIIQAADLYITKNNLNYEAQFDIISIIY
                     KGNAFAVEHIENAFYPRA"
     gene            1903..2544
                     /locus_tag="PK252_13420"
     CDS             1903..2544
                     /locus_tag="PK252_13420"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOT15753.1"
                     /translation="MRTIIKFFVIAVLVFIFPKGVQASGFEGKITMVKQSYYDTTYFT
                     YYVSNDRIRIEEYNKSKELQQVYLVNINKEEVFVINPQKKIYTKLNKVPANKNSEHFQ
                     VKKTHNSKNIKGVQCYQWRVKNKECNTEISYWVAKQNFDFFEKMVQVLNKTDKSWEYF
                     NKIPNTQGFFPMLYEERTLLRDEKNKTAVLNIEKKKVDSMKFVIPNDYQYFTI"
     gene            complement(2614..3522)
                     /locus_tag="PK252_13425"
     CDS             complement(2614..3522)
                     /locus_tag="PK252_13425"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOT15754.1"
                     /translation="MANIPPYLKQGDTIAIVATARFVTPGQIESSVNLLKEAGFNVEV
                     GKAVFNANNQLAGTDKERADDLQHFINDDKVKAIFCARGGYGTIRTIQQVNFSKLSTN
                     PKWIIGYSDVTVLHSILSNMNIASLHSPMLYKNPDFETSNILNEEVISILKGKKTEYH
                     FNAHPLNFQGRAQGKLVGGNLSVLYSLRGTPYDLQTSDCILFIEDVDEYLYHIDRMMM
                     NLKSSKMLSKLRGIIVGDMTDMHDNQIPFGKNAYEIIAEHVEDLCIPVCYGFPAGHGQ
                     VNKPLVLGHKVKLDIGAKCSLTMTGR"
     gene            3668..5704
                     /gene="metG"
                     /locus_tag="PK252_13430"
     CDS             3668..5704
                     /gene="metG"
                     /locus_tag="PK252_13430"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT15755.1"
                     /translation="MQKEFKRYTVTAALPYANGPVHLGHLAGVYVPADIYVRYLRLRN
                     RDVIFVCGSDEHGVPITIKAKQEGVTPQDIVDKYHNIIKESFEKFGISFDVYSRTSAP
                     IHYETASDFFKTLYEKGEFVEKESEQYFDEAANQFLADRYITGTCPRCGNEGAYGDQC
                     EKCGSSLSPTELINPVSKLSGGKPVLRKTSHWYLPLDKHEQWLRQWILEEHKEWKTNV
                     YGQCKSWLDSGLQPRAVSRDLDWGIPVPVDGAKGKVLYVWFDAPIGYISATRSIRPDW
                     EKYWKDKDTRLIHFIGKDNIVFHCIVFPAMLKAHGDYVLPENVPANEFLNLESDKIST
                     SRNWAVWLHEYLVDFPCKEDVLRYVLTANAPETKDNDFTWKDFQTRNNSELVGILGNF
                     VNRAMVLTHKYYEGKLPQIGQLNDQEKEIIENIASIKSLIEENLEQFKFREALKEFMN
                     LARIGNKYLADTEPWSLIKTNPEKVKDILNLSLQIAANIAILGEPFVPFTSLKLRNML
                     GIKKWSWDDAGKTDILPTGHQLNKPELLYAKIEDDVIEAQLKRLANIKAANAVATALV
                     TPQKPNINYDSFSAMDIRTGVILEAEIVPKTKKLLKLTIDTGIDKRTVVSGIAEYYTP
                     EQCIGQQVCILVNLEPRELKGITSNGMILMASDANGKLVFVSPTEKVKPGSEVK"
     gene            5753..6817
                     /gene="pdxA"
                     /locus_tag="PK252_13435"
     CDS             5753..6817
                     /gene="pdxA"
                     /locus_tag="PK252_13435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOT15756.1"
                     /translation="MKKVRLGITHGDVNGVGYELIINTFTDARMLEVCTPIIYGSAKV
                     AAYYRKTLNNNNVIINSVRTIEEINNKKLNLINCVDENIKVEIGKESQAAGEAAFQAL
                     EAAVKDFKAGKIDALVTCPINKFSIQSEAFNFHGHTDYLQDRFSTEEVLMFMVSGNLR
                     VGLVTEHVPLSKVSALVTKEKIISKLKLMQSSLIKDFCITNPKIAVLSLNPHAGDKGV
                     IGKEDDTVLIPAIEAAKEQGIFALGPFAADGFFGSANYKKFDAVLAMYHDQGLIPFKT
                     IAAGEGVNYSAGLPIVRTSPAHGTAFDIAGKGIADFSSFRNAVYMACDIFKNRSEYIQ
                     LSSNPLKPLSAQVNLDVDAE"
     gene            6861..8006
                     /locus_tag="PK252_13440"
     CDS             6861..8006
                     /locus_tag="PK252_13440"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOT15757.1"
                     /translation="MKHFLGLITIAAVALTACNQASKTDDGFLAVDTLAQQKNFQAEI
                     DGKKTDLFTLKNDSGMVVKITNYGARIVSVLVKDKNGKYDDVNLGYASIAEYQKDGMF
                     LGAAIGRYGNRIGKATFKLDGKEYKLAANDHGNTLHGGVKGFDKVVWDAVQNGDTLNL
                     SYTSPDMEEGFPGTLNVKLQYILTNNNEIVMNYEAVTDKNTVVNLTNHAYYNLSGEGN
                     GDILGNLLQVNAAQTTEVDTLLIPTGKLADVAGTPFDFNTPTAIGARIGEDNQQLKCG
                     GGYDHNWVLTKKEGELSLALRLTDTISGRCLEIFTTEPAIQIYVGNFMNGTVKGKTGK
                     PLNYRSAIAMEPQHFPDSPNKPNFPSTVLKPGQTYKQTSIVKFSVVK"
     gene            8102..9250
                     /gene="galK"
                     /locus_tag="PK252_13445"
     CDS             8102..9250
                     /gene="galK"
                     /locus_tag="PK252_13445"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT15758.1"
                     /translation="MNTNELVKKFESLYNQKPVIARAPGRVNLIGEHTDYNEGFVLPA
                     AVDKEIVFAIQKNETSKVQVFSMDMNQSATFDVNELKAQAGWVNYIIGVFAEVKKKGV
                     AVNGLNIVFAGDVPLGAGMSSSAALECALATGLNYIFGGPLSKIDLALAAQAAEHNYA
                     GVKCGLMDQYASIFGMPDHVIKLDCRTNKHEYFNFDFSDFALVLCDTNVKHSLASSEY
                     NTRRGQCEAGVKAIQVHHPNVKSLRDVTLQMLDEVKGSLDAVAYARCSYVVEENIRVE
                     KACEALKKNDFAAFGQFMYQSHEGLQHKYEVSCKELDFLVDCTRDNADVLGSRMMGGG
                     FGGCTINLIKRTAVDKFVSEIAPRYKKETGLDMKAYIVKIGAGARIGE"
BASE COUNT         2830 a         1696 c         1979 g         2906 t
ORIGIN      
        1 aggacttaca gtccgttaga acaacccacc gccttcaggc gtgtggtaca tgagtatatt
       61 tttcagaaat gatttgtttc ccattccatc tgcctttgtt taatatattt ttcagaaatg
      121 atttgtttcc cattccatct gcctttgttt aaaactaatt gtcctattcg ggcaaagtcg
      181 cgggctatgg cattaaagca acagtaggct ttttcgttgc cattagcatt gtcaaggctc
      241 cagagagcat cttttgaagc tccaagaggt cgccatattt tttcagaggc atattccgaa
      301 acgcttttgc cggtggcctt ttcaagaata aaagccagga gttgtgtgtt ggcgctcaaa
      361 taacgaaatt ttttacccgg ctgttcggtt atttttcttt ctaatatcag ctttttcagg
      421 tcattcccat agtatgcctt tgtagtaggc gaaaaagggc tggtgtagcg ctcgtcccaa
      481 tcgaggccac tgctcatggt tagtaagtgg cgtattgata aagctttgcc catgctatcg
      541 ttgaaagtag gcaggtaatg ccaaactggt tcgtcaaggc ttttgatgag gctgtcgtcc
      601 agggcgcaac caactaaaag cccaatgatg cttttggcta ccgaaaagga gtttgaaata
      661 caagtgtcgt tgaaattgcc aaaatattgt tcatgtacaa ttttaccatg ttgaacaacc
      721 agaaaagcat gggtttgtaa gcgaaacatg atatcctggt agtttttagg gacatcttta
      781 caattgtagc ctgtagcttt ctcccattcc tgcgtttggt ctttggaaac attcctgttt
      841 ttaaagaatt tgtaatcatc tgtattgaca ttgccatgta ataaggcttg ccgcagatat
      901 gaattggacg ggatgcagag aaaaatagca agtgcagcta ccaaaatagc aattgcggtg
      961 atgatttttt ttatcatatt gattgaattt tacagttttt tataaaaagc gcttttacgg
     1021 ggcaaactcg cgcaaaccaa gtcgtatgag tcttttatcc actgctctgt tagctttctg
     1081 ttcggagctt cttcaagcat taccgtattc caatgggttt tgttcatgtg gtagcccgga
     1141 agtacccatg aatattgttc gcggagagaa atggctttgt cagggtcgca tttgacgttc
     1201 atgctgaaag gcggctctaa gctaagcagc aaaaacattt tgttgtccac tttaaacact
     1261 aacgtatctt catcaaaagg gaaacattcg cttactccgg gaagtgaatt gcagtaattg
     1321 cgtacagttt ctatatccat attttataat cgaatttttg ctaagctctt ggataaaatg
     1381 cattttcgat atgttcaaca gcaaaagcat tgcctttgta aataatggaa ataatatcaa
     1441 attgtgcttc gtagttcagg ttgttttttg ttatgtataa gtctgccgcc tgaataataa
     1501 gcttttgttt tcggcgtgaa acagcttctg ctggcgatac aataaaatct tcggccctgg
     1561 tttttacttc cacaaaaacg agtgtttggt ctttcatagc cacaatgtca agttcgcggt
     1621 gcatcaattg ccagtttctc gaaagtatct tatatccttt attggcaagg tattgtgctg
     1681 ctatttcttc gcctttgtct ccaatggttt tagcgttggt catggtttag gatgtgtttg
     1741 tccaaagata aaaattctgt caggaatttt ggtttgttcg tcaatactta atataagttt
     1801 ataaaataaa aaaaacttgt ataaaaatcc atcgtaacat tgcataaaaa aggaatagtc
     1861 ttataagtgt atttttgggt tttaatttta aagaaggaaa acttgaggac aattattaaa
     1921 ttttttgtta tcgcagtgtt ggttttcatt ttcccaaaag gtgttcaggc ctcgggattt
     1981 gaaggaaaaa tcaccatggt gaaacagtcg tattacgaca ctacttactt tacttattat
     2041 gtttctaatg accgcatcag gatagaagag tataacaaat cgaaggagct acaacaggtg
     2101 tatcttgtaa acattaacaa agaggaggtt tttgttatca atccacaaaa aaagatatac
     2161 accaagctta ataaagttcc ggccaataaa aacagtgaac attttcaggt aaagaaaacc
     2221 cacaactcta agaatattaa aggtgttcaa tgttatcaat ggcgcgtgaa aaacaaagaa
     2281 tgtaacaccg aaatcagcta ttgggttgct aaacaaaatt ttgatttctt tgaaaagatg
     2341 gtccaagtcc ttaacaagac tgataaaagc tgggaatatt ttaataaaat acccaataca
     2401 caaggctttt ttccaatgct ttatgaagag cggactttgc ttcgtgacga aaagaacaaa
     2461 acggcggtgt tgaatattga aaagaaaaaa gtagattcca tgaagtttgt gatacctaat
     2521 gattatcaat attttaccat ataacttata ggctgaaatt ttccccaaaa tttggtcagg
     2581 ccggtgaaag gaaactcacc ggcttttctt tttttatcgt ccagtcattg tcagactgca
     2641 tttagcccca atatcaagct ttaccttgtg tcccaaaacc aaaggcttgt tgacttgtcc
     2701 atggccggcc gggaacccat agcaaacagg aatacagagg tcttcgacat gttcggcaat
     2761 gatttcgtaa gcatttttac caaaaggaat ctggttgtcg tgcatatcag tcatgtcgcc
     2821 aacaatgatg cccctaagtt tacttagcat tttgctgctt ttcaggttca tcatcatccg
     2881 gtcaatgtgg taaaggtatt cgtccacatc ttcgatgaac aggatgcaat ctgaggtttg
     2941 gaggtcgtaa ggtgtgccgc gcagactata taaaacagaa aggttgccgc ctacaagctt
     3001 tccctgagca cgaccctgga agttcagcgg atgggcattg aaatgatatt ctgttttttt
     3061 gccttttaag atagaaatga cctcttcgtt aaggatattt gaggtctcaa agtcagggtt
     3121 cttatacagc attggcgaat ggagcgatgc aatgttcatg ttgcttaaaa tagaatgaag
     3181 aacggtaaca tcactatatc caataatcca tttaggattt gtagagagtt ttgaaaaatt
     3241 aacctgctga attgttctta tagtaccgta tccacctctt gcacaaaata tagccttaac
     3301 tttatcgtca tttataaaat gttgcaaatc gtcagccctt tctttgtctg tacctgccaa
     3361 ttggttgttg gcattgaaca ctgctttgcc tacttctaca ttgaaccctg cttcttttag
     3421 aagattaacg gagctttcaa tttgaccggg tgtgacaaag cgcgcagtgg ctacaatagc
     3481 aattgtgtct ccttgtttaa gatatggtgg aatgttagcc atgttgcttt attattttca
     3541 caaaaataat cagtatttct ttgctaattc aataactgta gctttagttt tttatctttg
     3601 ctgtcttaaa aattattcca gaaaagtttg attttaagat atatctgtta gaaaattatt
     3661 ttttaccatg caaaaagaat ttaagcgtta tactgttaca gcagctttgc cttatgccaa
     3721 tggcccggta caccttgggc accttgcagg agtttatgtc cctgccgata tttatgtgcg
     3781 ttaccttagg ttaaggaacc gtgatgtcat atttgtctgc ggaagtgacg agcatggagt
     3841 cccaattacc attaaagcca agcaggaagg ggtaactcct caagatattg ttgataaata
     3901 ccacaacatt attaaagagt catttgaaaa gtttggaatt tcatttgatg tgtattctcg
     3961 caccagtgcc ccaattcatt acgaaactgc ctcggatttt ttcaaaactt tatatgaaaa
     4021 aggcgaattt gttgaaaaag aatctgaaca atattttgat gaagcggcaa accagtttct
     4081 tgctgacaga tatatcaccg gaacctgccc acgatgtgga aatgaaggcg cttatggcga
     4141 ccaatgcgag aaatgtggtt catcacttag ccccactgaa ctgataaacc ctgtatctaa
     4201 gcttagtgga ggtaaaccgg tattgcgaaa gacttctcac tggtatcttc cactcgacaa
     4261 gcatgagcaa tggctacgtc aatggatatt agaagagcat aaagaatgga aaaccaacgt
     4321 ttatggtcag tgtaaatcat ggcttgattc aggactccag cctcgtgcgg ttagccgtga
     4381 tttagattgg ggaatacctg ttcctgttga tggggctaag ggcaaagtat tgtatgtatg
     4441 gtttgatgca cctattggct atatatctgc tacacgttca ataagacctg actgggaaaa
     4501 gtattggaaa gataaagata cgcggttgat acattttatt gggaaagata atattgtgtt
     4561 ccattgtata gtttttccag ccatgcttaa agcgcatggc gattatgtat tgcccgaaaa
     4621 tgtcccggct aacgaattct taaacctcga aagcgacaaa atatcaacat ctcgcaattg
     4681 ggccgtttgg ttacatgagt atttggttga ttttccttgc aaagaggatg tgttgcgtta
     4741 cgttttgaca gccaatgcgc cagaaacaaa ggataatgac tttacctgga aagacttcca
     4801 aacccgcaat aatagcgaat tggttggtat ccttggaaac tttgtaaacc gtgctatggt
     4861 gcttactcat aaatattatg aaggaaagct ccctcagatt ggacaactta atgatcagga
     4921 aaaggaaatc attgaaaata tagcttctat aaagagcctg attgaggaaa accttgagca
     4981 gtttaagttt cgtgaagcgt tgaaagaatt tatgaacctt gcccgtattg gcaataaata
     5041 ccttgcagat actgagcctt ggtcgcttat caaaacaaac ccggaaaaag taaaagatat
     5101 tttgaactta tcattacaga tagcagccaa tattgctatt ctgggcgagc cttttgttcc
     5161 gtttacttct ttaaagctcc gcaatatgct tggtataaaa aaatggtcat gggatgatgc
     5221 cggaaaaacc gatattcttc caacagggca tcagttaaac aagccagaat tgctttatgc
     5281 caagattgag gatgatgtta ttgaagccca gttaaaacgt ttggcaaata taaaagctgc
     5341 aaatgctgtg gccactgctc ttgttactcc tcaaaaacca aatatcaatt atgacagttt
     5401 ttctgctatg gacattcgca caggcgttat tcttgaagct gagattgttc caaagacaaa
     5461 aaaattgctc aaactgacca ttgataccgg aatagataaa cgtactgttg tttcgggtat
     5521 tgcagaatac tatactcctg aacaatgcat tggtcagcag gtttgcattt tggttaatct
     5581 cgaacctcgt gagttgaagg gtattacttc aaacgggatg attttgatgg cttcagatgc
     5641 taacgggaag ctggtttttg tttctccgac agagaaagtt aagccgggtt ctgaagtgaa
     5701 gtagtttttt tatcttatta ttgtatcagt tttagctaaa aatatttcaa aaatgaaaaa
     5761 agtaagattg ggaattacgc atggcgatgt gaacggtgtt ggttacgaat taattataaa
     5821 tacctttact gacgctcgaa tgctcgaagt gtgtactcct attatatatg gttcggcaaa
     5881 ggtagcagca tactatcgca aaacccttaa taacaacaat gttataatca actcggtacg
     5941 tactattgaa gaaataaata ataaaaaatt gaacctcatc aattgcgttg acgaaaatat
     6001 taaggttgag ataggaaagg agtcgcaagc tgctggtgaa gccgcttttc aggcattgga
     6061 agctgctgtg aaagatttca aggctggtaa aattgacgct ttggtaactt gtcctatcaa
     6121 taaattcagc atccagtctg aagcatttaa ctttcatggg catacagact acctgcaaga
     6181 ccggttttcg accgaagagg tgttgatgtt tatggtaagt ggtaatttgc gcgttgggct
     6241 tgtaacagaa catgttcccc tttcaaaagt atcagcattg gtaacgaaag aaaaaattat
     6301 cagcaaacta aaactcatgc aaagttcttt gattaaagat ttttgcatta ctaatcccaa
     6361 aatagctgtt cttagtctca atcctcatgc cggagataag ggcgtaattg ggaaagaaga
     6421 tgacacagtg ctgattcctg ctattgaagc ggccaaagaa caaggtattt ttgcccttgg
     6481 cccatttgct gctgacggtt tctttggtag tgcgaattat aaaaaatttg atgcagtact
     6541 tgctatgtac catgaccagg ggcttatccc tttcaaaacc attgcagcag gggagggcgt
     6601 taattattct gccggattgc caatagtaag gacatcaccg gctcatggaa cagcttttga
     6661 tattgctggt aagggcattg ccgattttag ttcgtttcgt aatgctgttt atatggcctg
     6721 tgatattttt aaaaatagat ctgaatatat ccaactgtct tccaatccct tgaaaccttt
     6781 gtcggcccaa gtaaaccttg atgttgatgc ggaataaaat aaatgttata tttacgctta
     6841 aatttttaaa tatatctgca atgaaacact ttttgggatt aattactatt gctgctgttg
     6901 ctcttacagc atgcaaccag gcttcaaaaa ccgatgatgg ttttttggcc gttgatactt
     6961 tagctcagca aaaaaacttc caggctgaaa ttgatggtaa aaagactgac ctttttacgc
     7021 tgaaaaatga cagtggaatg gtagtgaaaa ttactaatta cggagctcgc attgtttctg
     7081 ttttggtaaa agacaaaaat ggtaaatatg acgatgtaaa ccttggctat gccagtattg
     7141 cagagtatca aaaggatggc atgttccttg gggctgctat tggccgctac gggaaccgca
     7201 ttggtaaagc tacatttaag ttagatggca aagagtacaa acttgctgca aacgatcatg
     7261 gcaatactct tcatggtggt gtaaaaggct ttgacaaagt agtatgggat gctgttcaaa
     7321 atggtgatac ccttaacctg agctatactt cgcctgatat ggaagaaggc tttcctggca
     7381 ctttgaatgt taaacttcag tacatcctga caaacaataa cgagatagtt atgaactatg
     7441 aagctgtaac agacaaaaat actgttgtaa accttactaa ccatgcttat tacaacctta
     7501 gtggcgaagg aaatggtgat attttaggca acttgctcca agttaatgca gcacagacta
     7561 cagaagttga tactttactt attcctactg gtaaattggc cgacgttgct ggtactccat
     7621 ttgatttcaa tacaccaact gctattggag caaggattgg agaagataac caacaactca
     7681 aatgtggagg cggatatgac cataactggg tgttgactaa gaaagaagga gaattgtcat
     7741 tggcattacg tttgacagat actatttcag gacgttgtct tgagattttc actactgagc
     7801 ctgccatcca aatttatgtt ggtaacttta tgaatggcac agtgaaagga aaaaccggga
     7861 aacctcttaa ttatagaagt gctatagcta tggaacctca gcatttccct gactctccaa
     7921 acaaaccaaa cttcccaagc acagtgctca aacctggcca aacatacaaa caaaccagta
     7981 ttgttaaatt ttctgtggta aaataagctt ttatataact aacctaatct aaaattaaag
     8041 ctgtaattgt tgcgtagtca gtttgaatac caatggttac agctttttta aatttaaaag
     8101 aatgaataca aacgaattgg taaagaaatt tgagtcgttg tacaatcaaa aaccagttat
     8161 tgcaagggct ccggggcggg taaaccttat cggcgaacat accgattata acgaaggttt
     8221 tgtcttacct gccgctgttg acaaagagat tgtgtttgct atccaaaaaa atgaaacctc
     8281 taaagtgcag gtcttttcta tggatatgaa ccaaagtgca acctttgatg tcaatgagct
     8341 caaagctcag gcagggtggg taaactacat tattggcgtt tttgctgagg tgaagaaaaa
     8401 aggtgttgct gtaaatggct tgaatattgt ttttgccgga gatgttcctc ttggagcagg
     8461 tatgtcatct tcagcagcgc tggagtgtgc tttggctacc gggctaaatt acatttttgg
     8521 aggtccgttg tcgaaaattg acttggcgct tgcggctcag gcagccgagc ataactatgc
     8581 cggagttaaa tgtggcttaa tggaccaata tgccagcatc tttggtatgc ctgaccatgt
     8641 gattaagctt gattgtcgca ccaacaagca tgagtatttc aattttgatt tctctgattt
     8701 tgcccttgtg ctttgcgata caaatgtaaa acactctctg gcttcatctg agtacaatac
     8761 acgtcgcgga caatgtgaag cgggcgtaaa agctattcag gtacatcatc caaatgtaaa
     8821 aagccttcgt gatgttactc ttcaaatgct tgatgaagtg aaaggctcat tagatgctgt
     8881 tgcttacgct cgttgttctt atgttgttga agaaaatatc agggtcgaaa aagcttgcga
     8941 ggcgttgaaa aagaatgatt ttgcagcttt tggccagttt atgtatcaat cacacgaagg
     9001 cttgcagcat aaatacgagg ttagctgcaa agagcttgac tttttagtag attgcacaag
     9061 agataacgct gatgtccttg gttcacgtat gatgggtggc ggatttggcg gttgtaccat
     9121 caacttaatc aagcgtactg cggtagataa gtttgtcagc gagatagctc cacgctataa
     9181 gaaagaaaca gggctggata tgaaagctta tattgttaaa attggcgctg gtgccagaat
     9241 tggtgaatga attctaaggt ttgttaaacg tcagttcaac ttaggaagaa agcttacgta
     9301 ttttatattt actggcatta actaaatatt ccttcctttt gccttgaagc aaaaggaaca
     9361 aaaattcaag gcttgccgta aattggctaa aaaaagcttt atcgacggct c
//
LOCUS       DAOSPF010000098         9256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7403_length_9256_cov_7.669818, whole genome shotgun sequence.
ACCESSION   DAOSPF010000098 DAOSPF010000000
VERSION     DAOSPF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7403_length_9256_cov_7.669818"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(647..1654)
                     /gene="gap"
                     /locus_tag="PK252_13450"
     CDS             complement(647..1654)
                     /gene="gap"
                     /locus_tag="PK252_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT15759.1"
                     /translation="MSKIKVGINGFGRIGRLVFRAAQERNDVEIVAVNDPFLDIDYLI
                     YMLQYDTVHGKFQGTAKNEGGKLVVNGKEVKFFAEKDPAAIAWGAAGAEYVVESTGVF
                     TTTEKAMAHIKGGAKRVVISAPSNDAPMFVMGVNHTSYTKDMVVVSNASCTTNCLAPV
                     AKVLNDKFGIIEGLMTTVHAATNTQKTVDAPSAKDWRGGRSILNNIIPSSTGAAKAVG
                     KVIPSLNGKLTGMAFRVPTADVSVVDLTVRLEKSATYDEIKAAIKAASENEMKGILGY
                     TEDSVVSTDFIHDARTSIFDAGAGIALNGNFVKVVSWYDNEWGYSNKVLDLIAHMDTV
                     K"
     gene            complement(1772..3418)
                     /locus_tag="PK252_13455"
     CDS             complement(1772..3418)
                     /locus_tag="PK252_13455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT15760.1"
                     /translation="MKKNVILFFISLMLTIDHGKSCTNILVSKGASVDNAVMISYAAD
                     SHELYGELYFRPAAKYPAGTQMDIIEWDIQKYRGKIRQARETYKVVGNMNEYQLAIGE
                     TTFGGKEELIDTTAVLDYGNLIYITLQRAKTARAAIDTIVALIKENGYRSSGESFSIS
                     DANEVWIMEIIGKGSPVIIKDKKGKEKKVYNKGAVWVAIRVPDGYICAHANQARIKRF
                     PLNDKANCLYSPDVISFARAKGWFIGPDSMFSFADTYAPLDFGAKRFCEARVYSIFRR
                     AAMSQNLSPDYVMGVHDAKPLPLFIKPDKKLSVSDVFALMRDHFEGTPMDMTQDIGAG
                     PYACPYRWRPLIWKVDSVAYFNERAISTQQTAFSFISQARSSMPRYVGGVLWFGVDDT
                     YSTVYVPMYSNINKIPIWFAPQNGDLYTYSDSSAFWAFNYVSNLAYSRYSDMIVDIVA
                     KQKILEDKFIAEQAKTELEATNIASVEGEAAAIEFLTQYSNSKSAETFYTWKKLGHDL
                     LVKYLDGNLKTKGHHVEHPLYPKWWYQQIINQTGDKLKMK"
     gene            complement(3709..5553)
                     /locus_tag="PK252_13460"
     CDS             complement(3709..5553)
                     /locus_tag="PK252_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT15761.1"
                     /translation="MGSVFIIYLALSVIILKKIYSESVASAETIIESQSQKYANALTA
                     DLNSYLNQTIGIANVFNASLQLDANTRKNIFRQTLYQTIGTLPDVMATWLNIQLFDID
                     PKWGKDYGRLRYTYYKVGNDSGFQVDIIDTAGHNFEGDYYKIRKNKRIEFSEPYYDFY
                     GHDSTRHLLMTSICVPLFYPDGIFWGMVGVDLNLDRFYAFLPKYCQYKKSFSMLLSSN
                     GSIVQHVDTTYNSKKLNEIMPLTIKKNNILDSISSGKSFTLTTNIDGEEYFLAFTSVT
                     LNKQINPWSLAIAVPVSSIKNKSNKALLFSIIILILGFVILFIVTYILTNFLAKPLQD
                     SISFAKRLGDGDLSANIDIGNHDELGQLAESLKLMALHLSHMVNEVDKGAEILSKTAK
                     SLSSSSKSLLSASYSQYDTTEKVNKSIETIVEYIQKNTEMSQSAETVSKEAGKKIKSS
                     VRQSVKAITSMHFIGEKMCSINNIALETNILALNAAVEAARAGEHGRGFSVVASEVRK
                     LAENSRKVSDEIADLLSQAQADTENAGNMLDLTIPEIEKSASLINQILSSNMTQYNNL
                     DSINKAMQNLNDVTRQNNDQAKRMAVFSDEIEKQSERLKQLLNKFKSV"
     gene            5860..6606
                     /gene="fabG"
                     /locus_tag="PK252_13465"
     CDS             5860..6606
                     /gene="fabG"
                     /locus_tag="PK252_13465"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT15762.1"
                     /translation="MNLLQGKTAIITGGARGIGKAIALKFASEGANIAITDLIYDDNV
                     KAFEAELNAMGIKAKAYASNAAKFDETQTVVNQIIADFGRVDILINNAGITKDGLLMR
                     MTEEQWDAVINVNLKSAFNFTKAVQMQMLKQKGGAIVNMSSVVGVSGNAGQANYSASK
                     AGMIGFTKSVAKELGSRNIRSNAIAPGFIITDMTHKLTEEQRNAWIENIPLKRGGTPE
                     DVANVALFLASDLSSYVNGQVINVCGGMNT"
     gene            6678..7682
                     /locus_tag="PK252_13470"
     CDS             6678..7682
                     /locus_tag="PK252_13470"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOT15763.1"
                     /translation="MLRFSNSFYILTKQVFVFIVIIVVSSCVVQEEIQIGTFVPSEIK
                     PDTSINNVVLVNHALFNGTYPQSSFYKDSLYTMAYFDGLSDILSNSVQYKLVDAPVQY
                     LKKSMNEQFKKLTLEELNVLALKTDADAAVVLENYQTTYTDPIKLIFDENYGYYGSLV
                     IENTSLWRVYKLKTGEKIDEYLLCDTLFFDAAGNTFEEAYSNFPKIDNAISQTCYILG
                     QKYGKRIANTWQIEKRTLFNCEKPDFNEASKLAKNGQWEKALLLWKKYINHKNRRLAS
                     LACYNIAVACEVMDNIDAALDWAAKSYFTYPNVWATKYISVLEKRNAFIIQINSSDKK
                     "
     gene            7679..8917
                     /locus_tag="PK252_13475"
     CDS             7679..8917
                     /locus_tag="PK252_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOT15764.1"
                     /translation="MNVILLIIIAIMVFDFILERVLSYLNSVKRNRAIPEELKDVYDE
                     ETHLKSLDYKWAYEKLGYLTSSLSFASILLMLLLGGFAFADNFCRQYTENPILLSLLY
                     FGILFLLSDLLNTPIDIYSTFSIEEKYGFNKTTPNIYILDKLKSYLIGIIIGGGLLSL
                     FVWFYQTTGPLYWIYIWLVFAAFSVFMAMFYSNLIVPLFNKQTPLEDGELRTAITEFA
                     NKAGFKLKNIYVIDGSKRSTKANAYFTGLGPKKRIVLYDTLIKQLSTEEIVAVLAHEI
                     GHYKKKHTSTGLLLGLVQTGLTLYILSLMLGNKSIYDALGVTKPGVHIGLIAFGMLYS
                     PISTIIGLFMNVVSRHNEYQADAYAESFGLGDALISGLKNLSRNNLSNLNPHPAYIFF
                     HYSHPSLLQRIRAIKNKQVN"
     gene            8998..9071
                     /locus_tag="PK252_13480"
     tRNA            8998..9071
                     /locus_tag="PK252_13480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9032..9034,aa:Thr,seq:cgt)
BASE COUNT         2957 a         1619 c         1698 g         2982 t
ORIGIN      
        1 aaaataaatt aaaaatcaaa ttattgtgtt tacacaaaat attttatact ctcttcctat
       61 taggtagaaa tctaaactat aagcaggcag aaaaacaagt ttcaaccttt cttttgttat
      121 tccaaaaaac ctttcacttt cttagcatgg tggtaacgta cattatcaag tatcatgaca
      181 acacgtttat tcgggtaact tttcagtact tttttcagaa acctcttaaa cgaatgcgta
      241 tttcctttgc ttgcaggttg caccgttact tgtcctgatt aataatttac agcacaaaaa
      301 agtgttacac tttctttgtg catgtttaca ttccttgaac ttgttgacat ttctcaaccc
      361 atgcaactat cattagcagt attacctaaa gagcattcgt ccttgaaaag gattacttta
      421 tttggagaaa cttcataaag ttttttatct ccttcacaaa gttttctcgt tgttcgttat
      481 cagcttctgg gtaataacct tttcccttct tatgtttaag gtttagtttt tttttaacct
      541 aaatcaagca ttaaaaacat aaatggggtt aaaaaatcag tagcgtttag aataaaaaaa
      601 accgccagat ttcaccagcg gttttgtata ttgaagaagc aaattcttac ttaacagtat
      661 ccatgtgggc gataaggtca aggactttgt ttgaataccc ccactcattg tcataccagc
      721 taacaacctt aacgaagtta ccgttaagag cgataccagc acctgcatca aagatagaag
      781 tacgtgcatc gtggatgaaa tcagtagaaa ctactgaatc ttcagtataa ccaaggatac
      841 ctttcatttc gttttcagaa gcagctttga tagcagcttt gatttcgtca taagtagctg
      901 atttttccaa acgtacagtt aagtcaacaa cagaaacgtc agcagtagga acacgaaaag
      961 ccatacctgt aagtttgcca ttcaatgaag ggataacttt acctacagct ttagcagccc
     1021 cagtagaaga tgggataata ttgttcaaaa tagaacggcc acctctccag tcttttgcag
     1081 aaggagcatc aacagttttt tgagtatttg tagcagcgtg aacagttgtc atcaagcctt
     1141 ctataatacc aaatttatcg ttgagcactt ttgcaacagg agcaagacag ttagttgtac
     1201 atgaagcgtt tgaaacaact accatatctt ttgtatatga agtgtggtta acacccatta
     1261 caaacatagg agcatcattt gaaggagcag aaataacaac tcttttagcg ccacctttaa
     1321 tgtgggccat agctttttct gtagtagtga aaactccagt tgattcaact acatattcag
     1381 caccagctgc accccatgca atagctgcag gatctttttc agcaaagaat tttacttcct
     1441 taccattaac tactaattta ccaccttcgt ttttagcagt tccttgaaat ttgccatgaa
     1501 cagtgtcata ctgcaacatg taaatcaaat aatcgatatc caaaaatggg tcgttaacag
     1561 caacgatttc tacatcgttt ctttcttgag ctgcacgaaa cacaagacgt ccaatacgtc
     1621 cgaaaccatt aatacctact ttaatttttg acatagtttt taatatttaa caagattaat
     1681 cgttatttta aaagacccgc aaagttatga atctttgttc aaacgattgc aaaaatcaag
     1741 ccatattttt ggctccccaa aaatattttt attatttcat tttcaattta tctccggttt
     1801 ggttaattat ttgttggtac caccatttgg gatataaggg atgttcaaca tggtggcctt
     1861 ttgtttttaa attcccgtct aaatatttga ccaataaatc atgaccaagc tttttccagg
     1921 tataaaaggt ttcggcactt ttgctattag agtattgagt aagaaattcg atagcagcag
     1981 cttcaccttc aacagaagca atattggtag cttcaagttc tgtttttgct tgttcagcaa
     2041 taaatttatc ttctaaaatt ttttgtttgg caacaatatc aactatcata tcagaatatc
     2101 tgctataagc caaattactc acataattga aagcccaaaa tgcagatgaa tcagaataag
     2161 tgtacaaatc tccattttgg ggggcaaacc aaatcgggat tttattgata tttgaataca
     2221 taggaacata aacagtgcta taagtatcat caacaccaaa ccacaacacc cctccaacat
     2281 atctgggcat agaagaccgg gcttggctaa taaacgaaaa tgcagtttgt tgagtagaaa
     2341 tagcccgctc gttgaaatac gctacagagt caactttcca aatcaatggg cgccagcggt
     2401 atggacaagc ataaggccca gccccgatat cctgggtcat atccataggg gttccttcaa
     2461 aatgatcgcg cattaaagca aatacatctg ataccgatag ttttttatca ggcttaataa
     2521 ataaaggaag aggttttgca tcatgaacac ccatgacata atctggggaa aggttttgcg
     2581 acattgcagc ccttctgaaa atgctatata ccctggcttc acaaaaacgc tttgccccaa
     2641 aatcgagagg agcataagtg tctgcaaaac taaacatact atctggacca ataaaccaac
     2701 cttttgctct tgcaaagcta atgacatcag gcgaataaag acaattagct ttgtcgttta
     2761 gagggaaccg tttgatacgg gcttgattgg catgggcaca aatatatcca tcaggcacac
     2821 gtatagctac ccaaacggcc cctttattat acaccttttt ttctttgcct tttttatctt
     2881 ttataattac aggagagcct ttgccaatta tttccattat ccatacttca ttagcgtcag
     2941 aaatggagaa agattctcct gagcttcggt aaccattttc ctttattaaa gctacaatag
     3001 tatcaatagc tgcccttgca gtttttgcac gttgtaaagt aatgtatata aggtttccgt
     3061 agtcaagaac agcggttgtg tcaataagtt cttcctttcc accaaaagtt gtttcaccaa
     3121 tggccagctg gtactcgttc atgttgccta ccactttata tgtttcgcgt gcctggcgaa
     3181 ttttgcccct atatttttga atatcccatt cgataatgtc catttgtgtg ccagccgggt
     3241 atttggctgc tggcctaaaa tataattcgc catacaattc gtgcgaatcg gcagcatagc
     3301 ttatcattac agcattatca acagaagcgc cttttgaaac caagatatta gtgcaggatt
     3361 tgccatgatc aatagtcaac atcaagctta taaaaaataa aattacattt tttttcatta
     3421 tcatattttt tggtatgatt attgtagata taagttttta taaaaaagtt ctttttaaaa
     3481 tcattgtaaa ttaatcagtt aaaaaatatt ttaatttatt ttatttaaaa ttaatttttg
     3541 gcacgattat tgatttaata ataaacagaa gagttcttaa taaaaatagt tagtaagtgt
     3601 tttcatagtt tagtgtttag tttttagtta gtttgttagt tagtgtgatt agtttgagaa
     3661 agtcgcgaaa ttctaccaag aatttccctt tctacaaact aatttttttt ataccgattt
     3721 gaatttgttg agcaattgct ttaacctctc tgattgtttt tcgatttcat cagaaaatac
     3781 agccattctt ttagcctgat cattgttttg gcgtgtaacg tcatttaagt tttgcatggc
     3841 tttatttatg gaatcaaggt tattatattg ggtcatgtta gacgataaaa tctggttgat
     3901 tagcgaagcg cttttttcga tttccggaat ggtaagatct aacatgtttc cagcattttc
     3961 ggtatcagct tgggcctgac taagcaaatc ggcaatttca tcagacacct ttctgctgtt
     4021 ttcggcaagt ttacggacct ctgaagccac caccgagaag cccctgccat gttcgccggc
     4081 acgcgcggct tcaaccgcag cgtttagagc aaggatatta gtttcgaggg cgatattgtt
     4141 gatcgaacac attttctcgc caataaaatg catactggta atggctttta cagattgcct
     4201 cacactactt ttaatttttt tcccggcttc ttttgatacc gtttcagcag attgggacat
     4261 ttctgtattt ttttggatat actcaacaat ggtttcgatg gatttgttta ctttttcggt
     4321 agtatcatat tgcgaataag aggcagaaag caggcttttt gaacttgaag atagcgattt
     4381 ggcagttttt gataatattt cagcaccttt atccacttca ttgaccatgt gcgacagatg
     4441 aagggccatc aattttaacg attcggccaa ttgcccaagt tcatcatgat tacctatgtc
     4501 tatattggca gataaatcgc catctcccag ccttttagca aacgagatac tgtcttgtaa
     4561 aggctttgca aggaaattgg tgaggatata agttacaata aataaaatta caaatcccaa
     4621 tatcaagata atgattgaaa acaataaagc tttgtttgat ttgtttttta tagatgaaac
     4681 gggaacagca atagccaacg accatgggtt tatttgcttg tttagcgtta cagatgtaaa
     4741 tgccaaaaag tattcttcac catctatgtt ggttgtaagg gtaaaacttt ttcctgacga
     4801 aatagaatca aggatattat ttttctttat ggttaaaggc atgatttcat taagcttttt
     4861 gctgttataa gttgtatcaa catgctgaac tatactacca ttgctggata gaagcattga
     4921 aaagcttttt ttgtattgac aatatttggg gaggaatgca taaaaacgat cgaggtttaa
     4981 atcaacacca accatccccc aaaatatacc atcaggataa aataaaggga cacaaatact
     5041 ggtcataagc aaatgacgtg tagaatcatg tccatagaaa tcataataag gttcgctaaa
     5101 ttcgatacgc ttatttttac ggattttata ataatcgcct tcaaagttgt ggccagcagt
     5161 gtcaataata tctacctgaa aaccgctgtc attgccaacc ttgtaataag tgtaacgcaa
     5221 acgcccatag tctttgcccc atttagggtc aatatcgaat aactggatat tgagccatgt
     5281 agccataaca tcaggcaaag tgccaatggt ttgatataaa gtctggcgga aaatattttt
     5341 tcgcgtattg gcatcaagct gtagagaagc attgaaaaca tttgctattc caatagtttg
     5401 gttcaggtat gaattgagat cagcggtaag ggcattagca tatttttggg attggctttc
     5461 aataattgtt tcagctgagg ccaccgactc tgaatatatt tttttgagga taataactga
     5521 aagagccagg tagatgatga aaacagaccc cattatgcta aaaataaacc taaaacgaag
     5581 gcttttattc ttgaatacag aaatatgcaa ggcggacaaa cggaatttac taatgttgat
     5641 ttttgaaaaa atattttgga aggatgtaac taaaaaaatg aaaaataatt ttagtttttc
     5701 aataatactg tgatgcatag cagggttaat atttactgag taaatataca aaaatattga
     5761 aatatcaata cgaaattgta attgaatgta gaacagcaca aaattttgat aaaacgattt
     5821 actttattta ctttgcgaaa aacttttaaa ctgataaaga tgaatttact tcaaggaaaa
     5881 acggccatta ttactggcgg tgccagggga ataggcaagg caattgcttt gaaatttgcc
     5941 tctgaaggtg ccaacatcgc tattactgac cttatttatg acgacaacgt aaaagctttt
     6001 gaagcagagc ttaacgctat gggtattaaa gcaaaagctt atgcatcaaa cgctgcaaaa
     6061 tttgacgaaa cacaaaccgt tgtaaatcaa attatagcag attttggaag ggttgatatt
     6121 cttattaaca acgcaggtat caccaaggac ggtttgctta tgcgtatgac cgaagaacaa
     6181 tgggatgcag ttattaatgt aaacttaaaa tcagctttta actttactaa agctgttcag
     6241 atgcaaatgc tcaagcaaaa aggcggagcc attgttaata tgagttcggt tgttggagtt
     6301 tctggtaatg ccggccaggc aaactattca gcttcaaaag caggcatgat tggttttact
     6361 aagtctgttg ctaaagaact tggttcaaga aatatcagaa gtaatgctat tgccccaggt
     6421 tttatcatta ctgatatgac acacaagcta acagaagaac aacgtaacgc atggattgaa
     6481 aatattccac taaaacgtgg cggaacacca gaagatgttg ccaatgttgc attattcctg
     6541 gcctctgact tatcttctta tgttaatgga caggttatta acgtttgcgg tggaatgaat
     6601 acttagaaga tattttgaaa tacattttta ttaaactgtt gctaattttg aaaacatttt
     6661 catcaaaaat gccgaaaatg ctaaggttta gcaacagttt ttatatttta accaaacaag
     6721 tctttgtttt tattgtaatt attgttgttt cttcgtgtgt agtgcaggag gaaatacaaa
     6781 taggtacatt tgtcccttct gaaatcaaac cagatacaag cataaataat gtagtgctgg
     6841 taaatcatgc cctttttaac gggacctatc cacaaagcag cttctacaaa gatagccttt
     6901 acactatggc ttattttgat gggctttcag acatactgtc aaattcggtt caatataagc
     6961 tggttgatgc acctgttcaa tacctgaaaa aaagtatgaa tgagcaattt aaaaagttaa
     7021 cattggaaga gctcaatgtt ttagctttaa agactgatgc agatgcagca gttgtgctcg
     7081 aaaattatca aacaacatat accgacccta taaagcttat atttgatgaa aattatggct
     7141 attatggcag tctggttatt gaaaatacat cgctttggcg ggtttataag cttaaaacag
     7201 gtgaaaaaat tgatgaatac ctgctgtgcg atactttgtt ttttgatgca gcaggtaaca
     7261 catttgaaga agcatacagt aattttccga aaattgacaa tgcaataagc caaacatgct
     7321 atattttggg gcaaaagtac ggaaagcgaa ttgccaatac ttggcaaata gagaaaagga
     7381 cattgttcaa ttgtgaaaaa cctgatttca atgaagcttc aaaacttgcc aaaaatggac
     7441 aatgggaaaa ggctttgcta ttatggaaaa aatatatcaa ccataaaaac aggcgtttgg
     7501 catcattagc ttgttataac atagccgtag cttgcgaagt aatggataat attgatgctg
     7561 cccttgattg ggctgcaaaa tcgtatttta cctaccccaa tgtttgggct acgaagtaca
     7621 tttcagtatt agaaaagaga aacgcgttta ttatacaaat caattcatct gataaaaaat
     7681 gaatgttata ttattgataa tcatagcaat aatggttttt gattttatcc tcgaaagggt
     7741 attatcatat ttaaactctg taaaacgtaa cagggcaatt cctgaggaat taaaggatgt
     7801 ttatgatgaa gaaacccatc tgaaatcatt ggattacaaa tgggcttatg agaagttggg
     7861 ttacctgacc tcaagtttat catttgcttc tattttgctc atgttgttgt tgggagggtt
     7921 tgcttttgct gataattttt gcagacaata tacagagaac cccattttat tgtctttact
     7981 gtattttgga atcttgtttt tgctttcgga cctgttaaat acaccaattg atatttattc
     8041 gacctttagc attgaagaaa aatatgggtt taataaaacc actccgaata tatatatatt
     8101 ggacaaacta aaaagctatc tgattgggat aatcatagga ggtggcttgt tgagcctttt
     8161 tgtttggttt taccaaacta ccggcccttt gtattggata tatatttggc ttgtttttgc
     8221 agccttttca gtatttatgg ctatgtttta ttcgaatttg attgttcctt tatttaataa
     8281 gcaaacccct ttggaagatg gagaattgcg tacagcaatt acagaattcg ccaacaaagc
     8341 aggctttaag cttaaaaata tttatgttat agatggctca aagcgttcga caaaagctaa
     8401 tgcttatttt acaggtttgg ggccaaaaaa aaggattgta ctttatgata cattaataaa
     8461 acagcttagt acagaagaaa tagtggctgt attggctcat gaaattggac attataaaaa
     8521 gaagcataca agtacaggct tgctgctggg attggtacaa accggattga cgctttatat
     8581 tctgtcactt atgttaggaa ataaaagtat ttatgatgct ttgggagtaa ccaaaccagg
     8641 ggttcatatt ggcttgatag ctttcgggat gttgtacagc ccaatatcga ccatcattgg
     8701 tttatttatg aatgttgtat cgcggcacaa cgaataccag gcagatgctt atgccgaaag
     8761 ttttgggttg ggagatgcac taatcagtgg attgaagaac ctttcgcgca ataacctcag
     8821 caacctgaac cctcatccgg cttatatctt ttttcactat tcgcatccat cgcttttaca
     8881 gcgtatcagg gcaataaaaa acaaacaagt taactaattg cttgtttggg ttaagtgaat
     8941 tttactattt ttgctgtgct tttaaataga gcaaggaaca aggaacaaag aataaacgcc
     9001 tctttagctc agttggttag agcagctgtt tcgtaaacag caggtcgggg gttcaaatcc
     9061 cctgagaggc tcagaaaaat tatcaattat ctaattacaa ataactaatt aaattagtat
     9121 atttgtaatt caaaaatgcg gaaatagact cagttggtag tctcggctca ggcgagatcc
     9181 caagctgagg gtcaaatata ggcggaagta gctcagttgg tagagcatca gcttcccaag
     9241 ctgagggtcg cgggtt
//
LOCUS       DAOSPF010000099         9255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7405_length_9255_cov_6.620000, whole genome shotgun sequence.
ACCESSION   DAOSPF010000099 DAOSPF010000000
VERSION     DAOSPF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_9255_cov_6.620000"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PK252_13485"
     CDS             <1..765
                     /locus_tag="PK252_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15765.1"
                     /translation="EDDCDQLTQVLMSAEIKDMMNKKFNLMAHYGIDSVKTAYPKEKY
                     YNAFEDNFRFQRTEYKSIKISVLDIDKELAAKMANDVVAYADSLIWRMRKSRGQKAYD
                     LAQFEYARFDSITKTTTDSMNKLTKLGLYDYKVLSKDLNKAYYSALLKGKTDLANKIK
                     QRMDLSAKYGIAYDELNLKLIHNVEQRSAMFYKVQEIKAELEQALPSKFIVESARVSE
                     KKAYPRKSLIVLASTVSTFFLTLILLIFVDTVKKYF"
     gene            778..2220
                     /locus_tag="PK252_13490"
     CDS             778..2220
                     /locus_tag="PK252_13490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT15766.1"
                     /translation="MKLFKKENIVIFILTVLYILANAKLYLLGFEWLWLLPIALWIIL
                     LGIFAMDSLMFLLLFLVPLSIPLSHFVPGLPFNMMLPTEPIILGLTFIFLIKAIREEY
                     FDKDIIFHPVTLAIFFNLFWILVTSFTSEIPGVSFKFLASRFWFVVVFFLIASQLFVR
                     FSNIIRYFWYYAAGLSIVVMFAVGRLAVIGLTKHQEANQVVRPFYNDHTDYAAAVTMI
                     LVFMVGLLFLKKRAKGLEKIFYIGSSLFFTIALILSYTRASWLSLVVALGFFVAMFLR
                     FRLKVIFFIIGVLVSLFLVFQNDIIIALERNKQDSDKDLSKHITSAANISTDASNMER
                     INRWHCAIKMFKERPLFGFGPGTYQFLYAPYQVKREMTIISTNRGDVGNSHSEYLGPL
                     AESGILGTLSFLFIIITTMATASKLYFRSKRRKVRVMAITLLVAMITYYTHGFMNNFL
                     DTDKLSALFWGFTAMIVALDVYHAKKLKKA"
     gene            complement(2217..2966)
                     /locus_tag="PK252_13495"
     CDS             complement(2217..2966)
                     /locus_tag="PK252_13495"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT15767.1"
                     /translation="MFFILSKLLFFLINPIVWVLSLIVTGLLLKKRKEAFYLSAAILL
                     FIFSNAGIFQFVSKKWEIESLPADSVKKHYTYAVVLGGMASGNTENGKMLFGESIDRI
                     LQAIILYKQGKIDTIFITGGSGLVWGQSKKEAPIIRKFCTDMGVKENCILIEDQSRNT
                     HENAIMTKALLNSCQGRILLITSAFHMRRSMGCFKKSGFEFDILSTNPLCRNQLYLDD
                     YFIPKTEIIEKWGILLKEITGSIVYKLAGYI"
     gene            complement(3228..4232)
                     /locus_tag="PK252_13500"
     CDS             complement(3228..4232)
                     /locus_tag="PK252_13500"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT15768.1"
                     /translation="MKVAVYNTKPYDKEYLEKAAAGTNHELVFLESPLKERSVKLAEG
                     CDAVCVFVNDNLNDKIIESLHCIGVKLIALRCAGFNNVDIVAAYQKNIKVLRVPAYSP
                     HAVAEHAIALILTLNRKTHKAYNRVREGNFSLDRLIGFDLNGKTVGVIGTGKIGAVFA
                     QLIKGFGCNILAYDKFENKELAGIGQYVPMDTLFNQSDIISLHCPLTPDTHNLVNAES
                     IAKMKKGVMIINTSRGKLIDTESAINGLKTGQIGYLGIDVYEQEESIFFKDLSESIIA
                     DDKIMRLMTFPNVLITAHQAFFTDNAMVQIANTTLQNITDFETGNIKHSNEVTPEMVK
                     "
     gene            complement(4261..4824)
                     /locus_tag="PK252_13505"
     CDS             complement(4261..4824)
                     /locus_tag="PK252_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOT15769.1"
                     /translation="MSRKIVIAVTGASGAIYAEKLLSALVTLHGQWEKAAVVFSKNAV
                     DVWEYELKKPIPQFNGIVQYNTDDFFAPFASGSAGFDTMIICPCSMGTLARIACGYSD
                     SLITRAADVMLKERRKLILAPRETPYSLIHLQNMLTLTQAGAIICPANPSFYSMPASV
                     DELCNTVIERILTLAGFNFEHFSWGNV"
     gene            complement(4831..5430)
                     /locus_tag="PK252_13510"
     CDS             complement(4831..5430)
                     /locus_tag="PK252_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOT15770.1"
                     /translation="MRRIFILALILFIKVAFVSAQYFEGGIILGITGSQMDGDRLGGY
                     NKPGVAAGMFVQRAASDKWKYRAELKYVMKGAAKITTKDDLETYRKTLHYAELPVMAI
                     MKIKNKGFIEMGLAFGYCGYATLNYGAGPEDATSTVKPLELSALGGVGYQMNEKVSFN
                     LRLNYSLTPVSHVSGNQTIWGVYGQFNNVLTLAVNYAIK"
     gene            5528..6130
                     /locus_tag="PK252_13515"
     CDS             5528..6130
                     /locus_tag="PK252_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOT15771.1"
                     /translation="MKSLVIASNNKHKKEEIEALLNNSIQLFTPKEIGCFDELPETDN
                     TLEGNALQKAMYIYEKFGYDCFADDTGLEVEALQGAPGVYSARYSTDEAPDIDEKLRP
                     EYNIQKLLRELKHKDNRKARFRTVICFVEKGKPVYFEGIVNGTIIDEKKGTDGFGYDP
                     VFIPDGYTKTFAEMDMATKNAISHRGRAVVKFIEYLSKTK"
     gene            6180..8513
                     /locus_tag="PK252_13520"
     CDS             6180..8513
                     /locus_tag="PK252_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT15772.1"
                     /translation="MPDKLKIAAITLLISLIIPGKIIATDIKLGEWQDYLSYTHVKKI
                     IDTENRVYCMTSNSLFYYDKTDNSINTISKVQGLTANNFANIAYWGEGKMLIIVYEDL
                     CIDFVQNNKVAVFPYIKNKTGINNKTINDIMLYNNRLYLATGFGIVVIDLQKQLVEDT
                     YTIGIGNKSEAVFSLAYDNTYFYASSKNMIFRAKKDEPFLADQSRWEFMKSTDTEFGE
                     IECFANKLFAIVKDPAVWAGNYIIAYDGKAWSKAAFPFDDYKTLKVSNNKMVLTGFKV
                     YTADESMNVSTTATFDNALDATMDKNNVIWIADQYKALIKYMPDGQYEIKNPNSPMFN
                     PVQTLDIADNQMWTVTGSNNGWVNQWSRQGFSSYTNNKWNSVNIYKESHNPLLDSIYD
                     FVDVKINPSNPSQVYLATWQKGLVKYENDQITIYNAGAGNSTIGENIYWPGRYWVSNL
                     SYDAYGNLWMTNPRNPKPLSVLKTDGKWKSFSIPGLISSYYLVDKLVATSWGDIWISN
                     INSSNLYVYSYKGTLDTESDDEKSPYSLSNLVNEGNALIASTVVYCITEDRDGNLWVG
                     TDAGPVEIFNANYFFDNGASASKVKIALNTEDNSAVFLLESERINCIAVDGGNRKWLG
                     TQNSGVYLVSPDGTNLIRHFTAENSPLFSNTIIDIKINSSTGEVFFATDRGLISYRSD
                     ATKGSDEFGNVYVFPNPVREDYTGDIAITNLITDAIVKITDISGNLVYQTRAKGGQAT
                     WDGKNLRGMRVNTGVYLVFCSNNDGTKTFVTKLLFIH"
     gene            8527..>9255
                     /locus_tag="PK252_13525"
     CDS             8527..>9255
                     /locus_tag="PK252_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15773.1"
                     /translation="MRSIFFLFLLIRFSSGAFSQTQLLSLKQCIDSAKQNLPALQLAS
                     ANRDAAKNISPDLHFSTEFSVKNGYLYADQRGTELNIRQYADWPGNWPLQNQYSSTYS
                     EMASAQYKLEEKQIIAGVKSAYYSALGAHLYYGMINNLLSEMMEALKNDTSWLFNDTI
                     LDTDYMALHDVLAQWQLKKEMAYDELIIADGKLKNTAGLKLETEPQDSTIQMYELEPA
                     PSGNNRAPANLLKSVEKARIKINDI"
BASE COUNT         2948 a         1711 c         1837 g         2759 t
ORIGIN      
        1 gaagatgact gtgaccaatt gacccaagtg ttgatgtctg ctgaaataaa agacatgatg
       61 aacaaaaaat tcaatctgat ggcccattat gggatagata gtgttaagac tgcttatcca
      121 aaggagaaat attataatgc atttgaagat aattttcggt ttcagcggac agagtacaag
      181 tcaattaaga tttctgtatt ggatatagat aaagaactgg cagctaagat ggcaaacgat
      241 gttgttgcgt atgcagattc tctaatatgg agaatgcgca aatcaagggg tcaaaaagca
      301 tatgatttgg ctcaatttga gtatgcccgc tttgattcta ttacaaagac gaccacagat
      361 agtatgaata agcttaccaa acttgggctt tacgattata aagttttgag caaggatctg
      421 aataaagctt attacagtgc acttttaaaa gggaaaaccg atttagcaaa taaaattaag
      481 caaagaatgg atttaagtgc taaatatgga atagcctatg atgaattaaa cctgaaatta
      541 attcataatg ttgagcaacg ttctgccatg ttttacaaag ttcaggaaat aaaagccgag
      601 ttagaacagg ccttaccaag taaatttatt gttgaaagcg ccagggtttc tgaaaagaaa
      661 gcttatccaa gaaaaagctt gatagtattg gcatcaactg tttcaacatt cttcctgact
      721 ttgattttat tgatttttgt tgatactgta aaaaagtatt tttaatatac caccttgttg
      781 aagcttttta agaaagaaaa tattgtcatt ttcattctca cagtactgta tattttagct
      841 aatgctaaat tatatttatt ggggtttgag tggttatggt tattgccaat tgcattatgg
      901 ataatcctgc ttggcatttt tgccatggat tctttaatgt ttttattgtt atttcttgtt
      961 cctttatcca taccgttaag ccattttgtg ccaggattgc cttttaacat gatgttgcct
     1021 acagagccaa ttatacttgg gctgacattt atatttttga ttaaggcaat acgtgaagag
     1081 tattttgata aagatattat ttttcatccg gttacattgg ctattttctt caatcttttt
     1141 tggatattag ttaccagttt taccagcgaa ataccaggtg tatcgtttaa gtttttggct
     1201 tcaaggtttt ggtttgttgt agtatttttt ttaattgctt cacaactttt tgtacgattc
     1261 agtaatatta tcaggtattt ttggtactat gcagctggtc ttagcattgt tgtaatgttt
     1321 gctgtaggta ggcttgctgt tatcgggttg accaagcacc aggaagccaa tcaggtggta
     1381 aggccatttt ataatgacca tacagattac gctgctgctg taaccatgat tttggtattt
     1441 atggtcggct tattatttct taaaaaaaga gctaaagggc ttgaaaaaat tttttatatt
     1501 gggtcttctt tatttttcac aattgctttg attttgtcat ataccagggc ctcgtggttg
     1561 agccttgtag tggctttagg tttttttgtg gccatgtttt tgcgttttcg gttgaaagta
     1621 atatttttta tcatcggagt attggtaagt ttgtttttgg tgtttcaaaa tgacattatc
     1681 attgcattgg aacgaaacaa gcaagactcg gacaaagacc tttcaaaaca tattacttca
     1741 gcggcaaata ttagcacaga tgcatctaat atggaacgta taaaccgttg gcattgtgca
     1801 attaagatgt ttaaagagcg tcctttgttt ggttttgggc caggcactta tcaatttttg
     1861 tacgctccgt atcaggtcaa aagagagatg actataatta gtacaaacag gggggatgtt
     1921 ggcaattcgc atagcgaata ccttgggcca ttggcagagt caggtatttt gggaacattg
     1981 tcatttttgt ttatcattat aactaccatg gctacggcat cgaagctgta tttcaggtca
     2041 aaaaggcgaa aagtgcgggt aatggctatc acgttgctgg tagccatgat aacttattac
     2101 acgcatgggt ttatgaacaa ttttctggat accgataagc tttctgcttt gttttggggc
     2161 tttacagcta tgatagtagc tttagatgtt tatcacgcaa aaaaactgaa aaaagcttaa
     2221 atgtaccctg ccaatttata gactattgaa cctgtaattt cttttaacaa aataccccat
     2281 ttttctatta tttctgtttt aggaataaaa taatcatcaa ggtacaattg atttctgcat
     2341 aaagggtttg tagaaagtat atcaaactca aaaccagatt ttttgaaaca gcccattgaa
     2401 cggcgcatgt gaaaagctga ggtaataagc aaaatacgcc cttggcagct attgagtaaa
     2461 gccttagtca taatagcatt ttcgtgagta ttgcgtgatt gatcttctat caagatgcag
     2521 ttttctttga cacccatatc ggtacaaaat ttgcgaatta taggggcttc tttcttagat
     2581 tgtccccaaa ccaaaccaga gccccctgta ataaaaatag tgtcaatttt gccttgttta
     2641 tagagtataa ttgcctgcaa tatccggtca atagattcgc caaagagcat tttgccattt
     2701 tctgtattcc cagaagccat acctccaagt acaactgcat aagtataatg tttttttaca
     2761 gagtcggcag gtaacgattc aatttcccat tttttagaaa caaactgaaa aattccagca
     2821 ttgctaaaaa tgaacaataa aattgcagca gataaataaa aagcttcttt tctttttttt
     2881 aacaataagc cggttacaat caaggataat acccaaacta tggggtttat caggaaaaac
     2941 aaaagctttg acagtataaa aaacatacgc taaggtttaa tgcataaaat ttgtgcaagt
     3001 tttttaatag tggtaaaaat acagattttt atcaatattc tgtccgatga ttttggggct
     3061 aagacagtaa caggatgccc tgccgaaccg tttttattga ccggctaaga tgaacggggg
     3121 ttaacattct cagtacaaac aactatataa caaaagcatg cgtcacagac gtgctatagc
     3181 tgaattccca gataaagtaa aacttaacct ggaaaattta agactcacta tttaaccatt
     3241 tcgggggtta cctcgttgga gtgcttgata ttgcctgttt caaagtcggt aatattctgc
     3301 aaagtagtat tggcaatctg taccatggca ttgtctgtaa aaaatgcctg gtgggcagtt
     3361 atcagcacat tagggaaagt catcaggcgc ataattttgt catccgcaat aatggactca
     3421 gacaaatctt taaaaaagat agattcttcc tgctcgtaaa catcgattcc caaatagcca
     3481 atctggccag ttttaaggcc atttatggcc gattcggtgt caataagctt gccgcggctg
     3541 gtattgataa tcataacccc ttttttcatc tttgctatac tttcggcatt gactaagttg
     3601 tgagtgtctg gtgttagcgg gcaatgcagt gagataatat cagactggtt aaacagggta
     3661 tccattggca catattggcc tatcccggcc aattctttat tttcaaactt atcgtatgcc
     3721 agaatattac agccaaaacc tttaatgagt tgggcaaata ctgccccaat cttgccagta
     3781 ccaataactc caacagtttt tccattaagg tcaaagccta tgagcctgtc gagtgaaaaa
     3841 tttccttcac ggacacggtt ataagcctta tgagtttttc ggttcaaagt aagtataagt
     3901 gctatagcat gttcggccac tgcatgaggg gagtaagccg gtacgcgcaa tactttaatg
     3961 tttttttgat aagcagctac aatgtccaca ttgttaaatc ctgcacaacg caaggcaatc
     4021 agtttaacac caatacaatg aagactttca ataattttgt cattaaggtt gtcattgaca
     4081 aaaacacaaa cggcgtcgca accttcggcc agttttactg acctttcttt taatggcgat
     4141 tcgagaaata ccaactcgtg atttgtgccg gcagcagctt tttcaagata ttctttgtcg
     4201 tatggcttgg tattatatac agcgactttc atcttataaa aatttaaaaa ctcttactaa
     4261 tcaaacattg ccccagctaa aatgttcaaa attaaagcct gccaacgtca aaatcctctc
     4321 tataactgta ttgcaaagct catcaaccga ggcaggcatg ctgtaaaacg aaggattggc
     4381 aggacaaatg atagccccgg cctgggtcag ggtaagcata ttttgtaaat gtataaggct
     4441 gtaaggcgtt tcgcgagggg ctaaaatgag tttcctgcgt tctttgagca tcacatcagc
     4501 cgcccgggta ataagtgagt cagaataacc acaagcaatg cgggccaaag tacccatact
     4561 gcaaggacaa ataatcatgg tgtcaaagcc agccgagcca ctggcaaatg gggcaaaaaa
     4621 gtcatcagtg ttatattgca caataccatt gaattgaggt attggctttt tcaactcata
     4681 ttcccagaca tccacggcat ttttagaaaa tacaacagcg gctttttccc attgtccgtg
     4741 caatgttaca agggccgaaa gcagtttttc ggcataaatg gcaccgctgg ccccggttac
     4801 tgcaatgaca atttttcgtg acatgcttgg ttattttata gcataattaa cagcgagggt
     4861 cagcacattg ttgaactggc cataaactcc ccaaatggtc tggttgccag aaacatggct
     4921 gacaggggtt agtgaataat tgagccgtaa attgaaagac actttttcat tcatttgata
     4981 acctaccccg ccaagagcag acaattccaa cggttttaca gttgatgtgg catcttcagg
     5041 ccctgcacca taatttaacg tagcataacc gcaatagcca aaagcaagtc ccatctcgat
     5101 aaagccttta ttcttgattt tcataatggc cattaccgga agctcagcat aatgcaacgt
     5161 tttgcggtaa gtttccaaat catcttttgt ggtaatctta gctgcccctt tcatcacgta
     5221 tttcagctct gcccggtatt tccatttgtc cgatgcagcg cgttgtacaa acataccagc
     5281 agccacaccg ggtttattgt atcccccaag cctgtcgcca tccatctggc tgccagtaat
     5341 accaagtatg atacctcctt caaaatactg ggctgaaaca aacgcaacct tgataaataa
     5401 tattagtgca agaataaaga ttcttctcat ttgtcatgtt ttgattagct aaggtaagat
     5461 tttaaagtga gaaaaaaata tttggcataa acaagtaaac attatcttta cggacaaatg
     5521 gtgcaaaatg aagtcgttag taattgccag caacaacaag cataaaaaag aagaaataga
     5581 ggctcttttg aacaacagta tccaactttt tacccctaaa gaaatcggat gctttgacga
     5641 attgcccgaa acagacaata cgcttgaagg aaatgccctc caaaaagcaa tgtatattta
     5701 tgaaaagttt ggctacgatt gttttgccga tgacacaggg cttgaagtag aagccctgca
     5761 aggtgccccg ggcgtttact cggccaggta cagtactgat gaggcccccg atattgacga
     5821 aaagctcagg ccagagtaca atattcaaaa gcttttgcgg gagctgaagc ataaagacaa
     5881 tcgtaaagcc cggtttcgca ctgttatatg ctttgttgag aaaggcaagc ctgtttattt
     5941 tgaaggtata gtaaacggga ccattattga cgaaaaaaaa ggaactgatg gatttggcta
     6001 cgaccctgta tttattcccg atggctatac taaaaccttt gctgaaatgg acatggcaac
     6061 aaagaacgct atcagccaca gaggcagggc tgttgtaaag tttatagagt atttgtctaa
     6121 aacaaagtaa aagatactat ttcattattc tcaacgtttt gaatggaaaa accgataata
     6181 tgcccgataa attgaaaatt gcagccataa cattgctgat aagccttatt atacctggaa
     6241 aaattatagc aacagatatc aagttaggcg aatggcaaga ctatttatca tacacgcatg
     6301 taaaaaagat tattgatact gaaaacaggg tgtattgcat gaccagtaac agcctttttt
     6361 attatgataa aaccgataac agcatcaata ccatttcaaa agtacaaggc ctgacagcca
     6421 ataactttgc caacattgcc tattggggcg aagggaaaat gctcattatt gtttacgaag
     6481 acctttgtat tgattttgtg caaaacaaca aagtcgcagt atttccttat atcaaaaaca
     6541 aaacgggcat aaacaataag accatcaacg atattatgct ttacaacaac cggctttact
     6601 tagctaccgg atttgggatt gttgtaattg acctgcaaaa acagcttgtt gaagacactt
     6661 atactattgg tataggcaat aaatcggagg cagtgttcag tcttgcttat gacaacactt
     6721 atttttatgc atcgtctaag aatatgattt tcagagcaaa aaaagacgag ccatttcttg
     6781 ccgaccagtc gaggtgggaa tttatgaaaa gtacagatac tgaatttggc gaaatagaat
     6841 gctttgccaa caaattgttt gccatagtta aagacccagc ggtatgggca gggaactaca
     6901 ttatagctta cgatggcaaa gcctggagca aagcggcatt tccttttgac gattataaaa
     6961 ccttaaaagt gagcaacaac aaaatggttt tgacaggttt caaggtatat acggctgacg
     7021 aatccatgaa tgtaagcacc acagccacat ttgacaatgc ccttgatgct actatggata
     7081 aaaacaatgt tatttggatt gccgaccaat acaaagcatt gataaaatat atgcccgatg
     7141 ggcaatatga aataaaaaac ccaaacagcc cgatgtttaa tccagttcag acattagata
     7201 ttgcagacaa ccagatgtgg acagtcacag gtagcaacaa cggatgggta aaccaatggt
     7261 cgagacaagg attttcgagc tacaccaaca acaaatggaa ttctgttaat atttataaag
     7321 aaagccacaa tccgctcctg gactcgatat atgactttgt agatgtaaaa attaaccctt
     7381 ccaacccttc acaagtatat ttggctacct ggcaaaaagg cttggttaaa tacgaaaatg
     7441 accaaataac aatttacaat gccggggctg gcaacagtac tattggcgaa aatatttact
     7501 ggccaggcag atactgggtt tcaaacctga gttatgatgc ttatggcaat ctttggatga
     7561 caaaccctcg aaatccaaag ccattatcag tattgaaaac cgatggcaaa tggaaaagtt
     7621 tttctatacc aggtttaatc tcaagttatt acctggttga caaattagtt gcaacaagct
     7681 ggggcgatat ttggataagc aatatcaact cttcaaatct ttatgtttac tcttataaag
     7741 gcaccctgga tacagaaagt gatgatgaaa agtctcctta tagcttatca aacttagtca
     7801 atgaaggcaa tgcattgatt gcatctacag tagtttattg cattaccgaa gaccgcgatg
     7861 gcaatttgtg ggtaggcact gatgcagggc cggttgaaat tttcaatgca aattattttt
     7921 tcgataacgg tgcaagtgct tctaaggtaa aaattgcact gaatactgaa gataattcag
     7981 cagtatttct gctcgaatca gaacgtatca attgtattgc cgtggatggg ggcaaccgca
     8041 aatggttagg aacacaaaac tccggtgtat atttggtttc gcctgatggt accaatctaa
     8101 tcaggcattt tacggcagaa aacagcccgt tattttcaaa caccattatt gacattaaaa
     8161 tcaatagtag cacaggtgaa gttttttttg ctacagacag agggctgata tcatatagga
     8221 gtgatgccac aaaaggtagc gatgagtttg gcaatgttta cgtatttcct aatcccgtac
     8281 gtgaagatta taccggagat atagccatta caaacttaat taccgatgct attgttaaaa
     8341 taactgatat atcagggaac ctggtatatc aaacaagagc taaaggcggc caggcaacat
     8401 gggatggcaa gaaccttcgt ggaatgaggg taaatacagg tgtttatctt gttttttgtt
     8461 ccaataatga tggtactaaa acatttgtaa ccaagttgtt atttattcat taacaataat
     8521 atgggcttga gaagtatctt ttttttgttt ttgctaatac gctttagttc cggagcgttt
     8581 tctcaaactc agctgcttag cctaaaacaa tgtattgatt cggcaaaaca aaacctgccg
     8641 gctttgcaat tagcctctgc caacagggac gcagccaaaa acatatctcc tgacctgcat
     8701 ttcagcacag aattttcggt aaaaaacggg tatttgtacg ctgaccaaag agggactgaa
     8761 ctaaatataa gacaatatgc tgactggcct ggtaactggc cattgcaaaa ccaatactcc
     8821 tcaacttaca gcgaaatggc ctctgcacaa tataaactcg aagagaagca aataatagca
     8881 ggggttaaaa gtgcctatta cagtgccctt ggggcacacc tgtactatgg gatgataaac
     8941 aatttactct cagaaatgat ggaagcattg aaaaatgata cctcctggct tttcaatgat
     9001 accattctgg atacagatta tatggcgttg catgatgtgt tggcccagtg gcaactgaaa
     9061 aaagaaatgg cttacgacga gctgattatt gcagatggca aacttaaaaa cacagccggc
     9121 cttaagctgg aaacagagcc tcaggactca accatacaga tgtatgagct tgaacctgcc
     9181 ccatcaggca ataacagggc cccggccaat cttcttaaat ctgttgaaaa agccagaata
     9241 aaaataaacg acata
//
LOCUS       DAOSPF010000100         9104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7588_length_9104_cov_6.574428, whole genome shotgun sequence.
ACCESSION   DAOSPF010000100 DAOSPF010000000
VERSION     DAOSPF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_9104_cov_6.574428"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PK252_13530"
     CDS             complement(<1..1053)
                     /locus_tag="PK252_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT15774.1"
                     /translation="MKTVVKIGKSTRVTLKLIAMTVMLVMGATSLYAKNNDTTATVSF
                     SGKITDAETKKALVYASVLVEGTNIGTIANSDGEYLLKVPKNKANGKVCFTHLGYKTL
                     TLEISSLKAENNAIALQPEIVSLQEIIVRLEDPATLIKEAIAKIPRNYSKSAAMYTGF
                     YREAIQQNKKYVSIAEAVLEAYKASYNNIFTDDKVKVLYGRKSQDVKKMDTIVVKLQG
                     GPLTPFFLDIAKNPENIFNEDFFKNYDLKLSGQISLDNERCYIIEFEQRKNVNLPLYK
                     GKIFIEVESLAITAIEYGLSEYGLEYASSMFVKKKPLTMRTDILGADYFVKYTKNNDK
                     WTLNYVRSELRFKCKWK"
     gene            1276..1701
                     /locus_tag="PK252_13535"
     CDS             1276..1701
                     /locus_tag="PK252_13535"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT15775.1"
                     /translation="MKYRVIKQFTFEAAHRLYENYQGKCTNNHGHSWQIALHLEADEL
                     DNKGMVIDFTEMKKLKEWIDNELDHTTILSSTDPMVDYIKDSKQRLFVVTGNPTSEAI
                     GRVIFEKAVEFFTNDHVKVAKVEIAETCTSMAQIINDKL"
     gene            1701..2093
                     /gene="queF"
                     /locus_tag="PK252_13540"
     CDS             1701..2093
                     /gene="queF"
                     /locus_tag="PK252_13540"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOT15776.1"
                     /translation="MMKNNKEKQFNFLGKKVTKPSKKLDTFETPANINYVKFESNELT
                     SFCPVTGQPDFSQFILEYKPAQKCIESKSLKLYLWSFREEAIFAESLANKMLEDIVDA
                     IKPEWCKVTIIQNVRGGLQLTAIAENKK"
     gene            2302..3435
                     /locus_tag="PK252_13545"
     CDS             2302..3435
                     /locus_tag="PK252_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15777.1"
                     /translation="MKWLKILAGSIIVVFLSLLLTTYVIWLGYPKKKINILILDKTVP
                     DLTYKKHASLVWVLNNSRFVKNDGSNYSLSKDYYGFHPLKPISDYQYEIKRISLESVD
                     SMANVYDAVYFADARGVYFNEWFRGFRQRTENSLIEGGFNQNDFLLLKAMHQKQKLVI
                     AEFNILQKPTSDLISYKAQELFGVASTGWIGCYYASLDSSNIPVNILNLYYEQTGNSW
                     SYSGEGVIFYNFNQVVVLSAKEHMNSAMPIISSKNNELMDKVPDVADFTSNFEVLTVA
                     DSMDVVAEFQLNVNSVGDSIISAMGIKSNFPAIIINKGKALNTVYFAGDFNDVDLPYY
                     SSRLANSRQLLKYLSSDKKKIFFNEYYFPLIENIFKKYTENKK"
     gene            3473..>3666
                     /locus_tag="PK252_13550"
     CDS             3473..>3666
                     /locus_tag="PK252_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15778.1"
                     /translation="MKKIILKLTLGLFVAGFACNAYSQLDDVTKVLNGGDADAQKLMQ
                     AYAEPMGKGFALSLTNGWYNT"
     assembly_gap    3667..3766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3767..4444
                     /locus_tag="PK252_13555"
     CDS             <3767..4444
                     /locus_tag="PK252_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15779.1"
                     /translation="SFVPMAQIQAGIGLPFGNEIIVRFMPTIKLNASSFNLKLGSWGV
                     GVKHDIKQWIPVAKEVPFWGLSALVSYTSFKATSSGVLLTPDESMYYADQLNKIDCSG
                     QGVELNASAMMFGIQASSDIPIINVYGGIGLVSASSNIKLTGNYPIPVHPTPQEAINR
                     GSSKFLVNTDPKVYKDPIDMKFSSKTAFRANIGLRLKLALLTIHGDISVCDSYTLYSA
                     GLGISFR"
     gene            complement(4557..5531)
                     /gene="pfkA"
                     /locus_tag="PK252_13560"
     CDS             complement(4557..5531)
                     /gene="pfkA"
                     /locus_tag="PK252_13560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT15780.1"
                     /translation="MRKIKKIGVLTSGGDAPGMNAAIRAVTRAGISNGLEVYGIMRGY
                     EGMIDADIRRLLSGDVSGILQKGGTILKTARSKRFREYEFRQQAYENLKAIGIDALVV
                     IGGDGSFKGATEFSLEFDIPVVGLPGTIDNDMFGTNYTIGYDTCLNTVMEAVDKIRDT
                     ASSHNRVFFIEVMGREAGFVALHGGIACGAEAILIPEVKGQLDSLMQLLSSPERQNKS
                     SIILVAEGHTEGTTFEIAEKVRQTFPSLDVRVTILGHIQRGGSPTAYDRVSASRLGVA
                     AVEALLDDQKSIMIGIQSGEIVHVPLSKTVKQNREVSQSLLDVAKRLV"
     gene            complement(5934..6806)
                     /locus_tag="PK252_13565"
     CDS             complement(5934..6806)
                     /locus_tag="PK252_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT15781.1"
                     /translation="MTLKRQLKNFFDDFTYFSPSARRASLVISALIVLSLFIPGIYKE
                     FSEPHNLQTQKQLNHIYDSLLASLQNSSTTKETKTYQITNINTATKEQWIALDVKPYI
                     AERIEKYLAHGGKFNKPEDVLKIYGFDSALFIQIQPYLFVETNKKETNNNIAHVKPPS
                     LVEINTADSTTIEKLPGIDPSFTKRILKYRSLLGGYYSKNQLLEVFGFNQQMLDKIAN
                     EIRIDTSAIRHISLNSAEARHLYRHPYIGPEKAKIILKYRQLGRNPLTLDFLAKEKVF
                     NEKEIEKVKPYIIH"
     gene            complement(6876..>9104)
                     /locus_tag="PK252_13570"
     CDS             complement(6876..>9104)
                     /locus_tag="PK252_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT15782.1"
                     /translation="EGKYLLQDNEVKIPKGGITDIDLLPYIKQSPNKKVLGRYRWYLG
                     LYNLSTPGSNKFFSRTLRKIGEAPVIYDYTLTQQSSKQMQLFLFNEGYFNAVVKDTAT
                     LDEKQNAKISYYVNPGKQYRTSEITYFFKDTLIKKIIYADTINCLIKKGEVFTTLKLE
                     NERARIERYVRNQGYFGFSKEYISYEADTSGGNNSVKLKIEIGNVIERNEAGEINEHN
                     HIQYRLKDIQVWVENSRSIDSADYQLDKNMFDVTSIGDVEFIYREDKTVKPKLISNAL
                     YLIEGKLFIQDDLDATYQALMALRVFKFVNISFYEAARDTTDSIVPINCRIQLTTNAA
                     QSIQTEGELTNRTGFGVAGSINYQHRNVFKGAETFDFKIKGASEAVKSTNDIDLKYLL
                     DLSASASLKLPKIIIPFNLANFQRENNPVTNISLSYAYLKQLNYSNRVANINYNYQWK
                     NKRNGAFTVYPADINFIKILEIDTAFKAGIDTTYLRYSFMNHLVAATGINYVFSNQKP
                     KVNANFWYLRTNLESSGNSAYFFMNMLKEPRNKENSYEIMGIPFAQYLKFDIDIRRYF
                     PTFRNDKVVLRLYSGIAYPYGNSKSIPFERLYFAGGSNSMRAWSVRSLGPGSYKDVKQ
                     ASFPDKASELKIESNLEYRFKMFWKLEGTLFVDAGNIWSISKNEEREDAKFDLKTFIP
                     QLAVGGGWGARIDFSFFILRIDIAAKLRDPSMPEGKRWIPKYEKTFSQIMNIVFGIGY
                     PF"
BASE COUNT         2686 a         1538 c         1674 g         3106 t
ORIGIN      
        1 tttccattta catttaaatc tcaattcaga acgtacataa ttcaaagtcc acttgtcgtt
       61 gtttttggta tattttacaa agtaatctgc ccctaaaata tctgtccgca tagtcaatgg
      121 tttctttttt acaaacatgc tgctggcata ttcgagccca tattcgctca aaccgtattc
      181 tattgctgta attgcaaggc tttctacttc aataaatatt ttacctttat acaggggcaa
      241 atttacattt tttctttgtt caaattcaat tatgtaacat ctttcgttgt ctaatgaaat
      301 ttgtcctgat aattttaagt cgtaattttt gaaaaagtct tcattgaaaa tgttttcagg
      361 gtttttggca atgtcgagga aaaacggggt aagcgggcca ccctggagtt ttaccacaat
      421 agtgtccatt ttcttaacgt cctggctttt tcggccatac aatactttta ccttatcatc
      481 agtaaatatg ttgttgtaag aggccttgta agcttctaat acagcttctg caatagatac
      541 atattttttg ttttgttgga tagcttcacg gtaaaagcct gtgtacatgg ctgctgattt
      601 gctgtaattg cgtgggattt tagcaatagc ttcttttata agcgttgccg ggtcttcaag
      661 tcgtacaatg atttcttgta aagaaacaat ctcgggttgt aaagcaattg cattgttttc
      721 agcctttaaa ctgctgattt caagggttaa agtcttatat ccaagatgag taaagcaaac
      781 ttttccattt gctttgtttt tgggtacctt taacaggtat tccccgtcag agttggcaat
      841 agtgccaatg ttggttcctt caacaagcac acttgcataa acaagtgctt tttttgtttc
      901 tgcatctgtt atttttccag aaaatgaaac ggttgctgtg gtatcattat tttttgcgta
      961 taatgatgtt gcacccatga ctagcatgac agtcatggca atgagtttca atgtaactct
     1021 tgtgctttta ccaattttaa ctacagtttt catggcatgt aaatttaaga tttatactaa
     1081 gttttcttat tgtttttgtt gaaaatttta ccggtacttt cttttagtac ttcacaatta
     1141 ttttcatttc tacccctatc gaaaattgct tatttttgca ctattcttta taaagccctg
     1201 aataatgtcg ctttttggct aaaaatgagc tatatttgaa ttagttaggg ctttgcgtca
     1261 caaaccgatt ttttgatgaa atacagagtt attaaacaat ttacatttga agctgcccat
     1321 aggttgtacg aaaattacca gggcaagtgt accaacaatc atgggcattc atggcagata
     1381 gctttgcatc ttgaggctga tgagttggat aataagggga tggtcattga ttttaccgaa
     1441 atgaaaaagc tcaaagaatg gatagacaac gagctggatc atacaacaat cttgtcttcg
     1501 acagacccaa tggttgatta tattaaagat tccaagcaac ggttgtttgt tgtaacaggc
     1561 aatcccacat ctgaagccat tgggcgggtg atatttgaaa aagcagttga gttttttacc
     1621 aatgatcatg taaaagtggc taaggtagaa attgctgaaa catgcacatc tatggcacaa
     1681 ataatcaatg ataagttata atgatgaaga ataataaaga aaagcaattt aactttcttg
     1741 gtaaaaaagt aactaagccg tctaagaagc tcgatacttt tgaaacacct gcaaatatta
     1801 attatgtcaa atttgaaagc aacgaattga ccagtttttg ccctgttacg ggacagcctg
     1861 attttagcca gtttattctg gaatataaac cggctcaaaa gtgcatagaa agcaagagtt
     1921 tgaagcttta tttgtggtca ttcagggagg aagccatttt tgctgaaagt ttggcaaata
     1981 aaatgctgga agatattgtt gatgctatta aacctgaatg gtgcaaggtt actatcatac
     2041 agaatgtaag gggaggttta caattgacag ctatagccga gaataagaaa taatttttta
     2101 ttttttgtca aaaaaatatt acatttaaaa aacgaaatat tggaagattt cagcattgga
     2161 taaatgaagt tgagataaat ctggctttca aaagaggttg aatgtttttt aatggtcgat
     2221 taagcataga ttgatgagat agcctgaatt cttcagaaat aaaaagtttt cagtgaatta
     2281 tttactaata tatccaatac aatgaagtgg ttaaaaatat tagcagggag cataatagta
     2341 gtatttcttt cattattact tactacttat gtcatttggt taggatatcc caaaaagaaa
     2401 attaatatcc ttattttaga taaaacagta ccagacctta cttataaaaa gcatgcctcg
     2461 ttggtttggg tgcttaacaa cagcaggttt gtcaagaatg atggcagtaa ttatagctta
     2521 tcaaaggatt attatggttt tcacccattg aaacctatca gcgattatca atatgagata
     2581 aaacgcataa gccttgaatc tgtagatagt atggccaatg tttacgatgc tgtttatttt
     2641 gccgatgcac ggggtgttta tttcaatgaa tggttcaggg ggtttaggca acgaacagaa
     2701 aattctttga tagaaggggg ctttaatcaa aatgattttc tcttactaaa ggcaatgcac
     2761 caaaaacaaa agttggtgat agctgaattt aatattttgc aaaagccgac ttcagacctt
     2821 atcagctaca aagcacaaga actgtttggt gtagcatcta caggatggat tggttgctat
     2881 tatgcttctt tagactcttc caatattcct gtgaatattc ttaacctgta ttatgaacaa
     2941 actggaaata gttggagtta tagtggagaa ggcgtaatat tttacaattt caaccaggta
     3001 gttgtgttgt cggcaaaaga acacatgaat tcagcaatgc ctattatttc ttcaaaaaac
     3061 aatgaattga tggataaagt ccctgatgtt gctgatttta cttccaattt tgaagtgcta
     3121 actgttgcag attcaatgga tgttgttgcc gaatttcagc ttaacgtgaa ttctgttggc
     3181 gactccatca tttctgctat gggcattaag tctaactttc cggctatcat tatcaacaaa
     3241 gggaaagctt tgaatacggt atattttgcc ggagatttta atgatgttga tttaccatat
     3301 tattcttcga ggttggccaa tagcaggcaa ttacttaaat atctctcaag tgataagaag
     3361 aaaatatttt ttaacgagta ttattttcct ttgatagaaa acattttcaa gaaatatact
     3421 gaaaacaaga aatgaaataa ataaccaaat aacctataaa taaataaaca ttatgaagaa
     3481 aataatctta aaacttacgc ttgggctatt tgttgcaggt tttgcatgca atgcatacag
     3541 ccagcttgat gatgtgacaa aagtgttgaa cggaggcgat gctgatgccc aaaagcttat
     3601 gcaagcgtat gcagagccaa tgggaaaagg ctttgccctt agcttgacca atggatggta
     3661 caatacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagct ttgttcctat
     3781 ggctcaaatt caggctggca ttggtttgcc gtttggcaat gaaattattg tcaggtttat
     3841 gccaacaatt aaactgaatg caagttcttt taatttgaaa ttaggctcat ggggtgttgg
     3901 tgttaaacac gacattaaac aatggattcc ggtggcaaaa gaagttcctt tttggggctt
     3961 aagtgctttg gtatcttata caagttttaa agcaacttct tcaggagtac ttttaactcc
     4021 agatgaaagc atgtattatg ctgatcagct taataaaata gattgcagtg gacaaggagt
     4081 tgagcttaat gccagtgcta tgatgtttgg tatccaggct tcttcagata ttccaattat
     4141 taatgtttat ggaggtattg gacttgtttc agcttcttct aatattaagt taacaggtaa
     4201 ttatcctatt cctgtacatc caactccaca agaagctatc aacagaggct cttcaaaatt
     4261 tttggttaat actgatccta aagtttataa agatcctata gacatgaaat tttcttcaaa
     4321 aactgctttt cgtgccaata ttggtttgcg cttgaaatta gcacttctta ctattcatgg
     4381 agatatttca gtgtgtgata gctacactct ttactctgct gggcttggta ttagttttag
     4441 gtaatattta cacttagata taagtaaaaa ggctgtttca ttgagacagc ctttttttat
     4501 aataaaagcc cttgcaaata ataatgttta caagggcttt tatacattta gctatattat
     4561 accagtcttt tagccacatc gagcaacgat tggctaactt cacggttttg tttaacagtt
     4621 ttacttagag gtacatgaac tatttctcca ctctgaatgc ctatcataat gcttttctga
     4681 tcatccaaaa gtgcttcaac agctgctacg cctaatcgac tggcagacac acgatcgtag
     4741 gctgtggggc ttccgcctcg ctggatatgc cctaagatgg taactcgtac atcaaggctg
     4801 ggaaatgttt gacgcacttt ttctgctatt tcaaaggtag taccttcggt atgaccttct
     4861 gcaactaaga taatgctcga tttgttttgc ctttcgggag aagacaagag ttgcatcaaa
     4921 ctgtcaagtt gtccttttac ttcagggata agaatagctt ctgctccaca ggctatacct
     4981 ccatgcaaag ccacaaatcc tgcttcgcgc cccattactt caataaagaa cacccgattg
     5041 tgcgaacttg ctgtatcccg gattttatca acagcttcca ttactgtatt taagcaagtg
     5101 tcgtaaccta tggtataatt agtaccaaac atatcattgt caatagtgcc tggcaaccct
     5161 acaacaggta tatcaaattc gagcgaaaac tcagtagcac ccttaaaaga gccatcacca
     5221 ccaattacca ctaatgcatc aatcccaatt gctttgagat tttcataagc ttgctgacgg
     5281 aattcatatt cgcgaaaacg cttgcttcgt gcagttttta atatagtgcc acccttttga
     5341 aggattccac ttacatcgcc cgaaagtaac cgacgaatgt cggcatctat cattccttcg
     5401 tatccccgca ttatgccata aacttcaagc ccgttgctaa ttccggcacg tgttacagca
     5461 cgtatggcag cattcattcc gggggcatct cctcccgatg ttaaaactcc aattttttta
     5521 atttttctca tttctatatt ttatttcaac ttccaatgta aaatatttta ctttttataa
     5581 acctttcccg tcatagacgg gactatctgg tttagaaatt gtttgatgca attttttaat
     5641 tgcaggtttt caatttctta tgcttaagca tttgttaata tggcattaaa cattgatagc
     5701 aaaaatcata caacaaaaat tatacaaaaa gcacaggtgc cctaaaaaat tacggtcaaa
     5761 aacaaaaggg atgcatgtat taattttttt taagatttta tttgtggtgt ctcaaatatg
     5821 ctttttctga ggtggtgtaa gtaataaata gctgattttt aaagtcttat tgttcgaata
     5881 ttgtcaaata aataattttt aagggcaaaa ataaaattta aattgcagta tttttaatgg
     5941 ataatataag gctttacttt ttctatttct ttttcattga atactttttc tttggcaaga
     6001 aaatctaaag ttaatgggtt tctcccaagt tggcggtatt tcaatattat tttagctttt
     6061 tcgggcccaa tatacggatg gcggtataag tgcctggctt ctgctgaatt taaagatata
     6121 tgacgaattg cagatgtgtc aatgcgtatt tcgttggcta ttttatcgag catttgttgg
     6181 ttaaaaccaa atacttccag caattgattt tttgaataat acccaccaag caaactacgg
     6241 tatttcagga tacgttttgt gaaagaaggg tctatacctg gcagtttttc tatggtagta
     6301 ctgtcggcag tgttaatttc taccaatgat ggtggtttta catgtgctat attattgttt
     6361 gtttcttttt tattggtttc tacaaataaa taaggttgaa tttgaataaa caaagcagaa
     6421 tcaaagccat aaattttcaa aacatcttcg ggtttattga atttaccacc atgagcgagg
     6481 tatttttcaa tcctttcggc aatgtatggc ttaacatcca gggctatcca ttgctctttt
     6541 gtggcagtgt tgatgttggt gatttggtat gtctttgttt ctttagttgt tgaagagttt
     6601 tgcagcgagg ctaataagct gtcgtatatg tggttaagtt gtttttgggt ttgtaagtta
     6661 tgtggctcgc taaactcttt gtaaatacct ggaataaata atgaaagtac aattaatgcg
     6721 gatattacca aagaagccct tcgtgctgaa ggcgaaaaat aggtaaaatc gtcaaagaaa
     6781 ttctttaact gtcttttaag agtcatagtt tataaggttt gggtatttca aatataagca
     6841 tttgaaataa attcccaaaa cttgtaatgc gaattttaga aaggataacc aatgccgaaa
     6901 acaatgttca tgatttgaga aaatgttttt tcatattttg gtatccacct tttaccttct
     6961 ggcattgaag ggtcgcgaag tttagcggct atatctattc gtaggataaa gaaagaaaaa
     7021 tcaatacggg caccccaacc tccaccaacg gctaattgag gaataaatgt ttttaggtca
     7081 aattttgcat cttcacgttc ttcattttta ctaatagacc agatgttgcc agcatcaaca
     7141 aacaaggtgc cttccagttt ccaaaacatt ttaaaacggt attcgaggtt gctttcaatt
     7201 ttcaattcag aggctttatc cgggaagctg gcttgtttca catctttata tgatccaggg
     7261 cctaaactgc gaacagacca tgcgcgcatg ctatttgaac ctcctgcaaa atataacctt
     7321 tcaaaaggaa tactttttga atttccataa ggatatgcaa tgcccgaata caaacgtaga
     7381 acaaccttat cgtttctgaa tgttggaaaa tatctacgaa tgtctatgtc aaattttaaa
     7441 tactgggcaa aggggattcc cattatttca tagctatttt ctttgtttct gggttctttt
     7501 aacatattca taaaaaaata tgctgaattt ccggatgatt caagatttgt ccgtaaatac
     7561 caaaaattgg catttacttt tggtttttga ttgctgaata catagttaat tcctgtagca
     7621 gcaacaaggt ggttcataaa actatatctc aaatatgtgg tgtcaatgcc ggctttgaat
     7681 gccgtgtcaa tttctaatat tttgataaag ttaatgtctg ccggataaac agtaaaagcc
     7741 ccattccgct tgtttttcca ttgatagtta tagttgatgt tggctaccct gttagaataa
     7801 tttaattgtt taaggtaagc gtatgataat gaaatgttgg tgaccgggtt gttttcgcgt
     7861 tggaaattgg ccaggttgaa cgggataatt attttaggca atttcaaact ggcagatgca
     7921 cttaagtcaa gcaaatattt caaatcaatg tcattggtac ttttaaccgc ttcagaagct
     7981 cctttgattt taaagtcaaa agtctcagcg cctttaaaaa cgttacggtg ctgataatta
     8041 atacttccag ctaccccaaa cccggtacgg ttggtaagtt ccccttcagt ttgtatagac
     8101 tgtgcagcat tggtagtaag ctgtatccgg caatttatcg gtacaatgct gtctgttgtg
     8161 tctctggctg cttcgtaaaa gcttatgttt acaaatttga ataccctcag ggccatcagt
     8221 gcctggtatg tagcatcgag gtcgtcttgg atgaaaagct ttccttctat aaggtatagg
     8281 gcatttgaaa tgagttttgg ctttaccgtt ttatcttcgc ggtaaataaa ctctacatcg
     8341 cctatagaag taacatcaaa catgttttta tcaagttgat agtctgccga atcaatactc
     8401 cggctatttt ctacccatac ctgtatatct ttcagacgat attgaatatg attgtgctcg
     8461 tttatttctc cggcttcgtt gcgttcaata acatttccta tttcaatttt caatttaacg
     8521 ctattgttgc cgccagaagt atcggcttca taagaaatat attcttttga gaaaccaaaa
     8581 tatccttggt ttctaacata acgctctatc cgtgcccgct cattttccag tttaagtgtg
     8641 gtgaaaactt cgcctttttt tatcaggcaa ttgatagtat cagcgtatat gatttttttt
     8701 atcaatgtgt ctttaaaaaa atacgttatt tcgctggtac gatactgttt cccgggattg
     8761 acataatagc tgatttttgc attttgcttt tcgtctaaag tggcggtgtc ttttaccaca
     8821 gcattgaaat atccttcgtt gaacaaaaat aactgcatct gctttgagct ttgctgcgta
     8881 agggtatagt cgtatataac aggagcttcg cctattttac gtaatgtgcg gctaaaaaac
     8941 ttgttgctgc cgggggtgga taaattgtat aaacctaagt accaacggta tctgcctaat
     9001 acttttttgt tgggtgattg tttgatatag ggtaaaaggt caatatctgt tattccccct
     9061 ttaggaattt ttacttcgtt gtcctgtaaa agatatttgc cctc
//
LOCUS       DAOSPF010000101         9080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7616_length_9080_cov_7.165540, whole genome shotgun sequence.
ACCESSION   DAOSPF010000101 DAOSPF010000000
VERSION     DAOSPF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_9080_cov_7.165540"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..468
                     /locus_tag="PK252_13575"
     CDS             91..468
                     /locus_tag="PK252_13575"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT15783.1"
                     /translation="MQNDFLEIEGTKTMPAISFKEGKLDIIGRSIPIDSCEWFAPLLN
                     VMYAYKAKKGEKLEINIQLDFLNSESNRTLLNLLTIAEKIYNEGHAVHVKWYYKPNDP
                     VMFEQGNIFQSLVELPFTFEPIV"
     gene            591..2702
                     /locus_tag="PK252_13580"
     CDS             591..2702
                     /locus_tag="PK252_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOT15784.1"
                     /translation="MKKHALILFCALSVNAIAENGYNLWLRYKPVNHGAVKKEYLSSL
                     SSIEVKTEQENKQVIENELNTAIKSMLPGRSNSKGNKKLFILSAKENQQVGKNFKTYL
                     SSLGNEGYCIIDTTINNQQAIYIIANTGKGCVYGTFKLLSLMQEEKSLKNIRIQSVPK
                     IKYRMLNHWDNPDGSVERGYAGKSLWYGKTKVSEQRLIDYARANASVGINSVVINNVN
                     ASPAMMNEENIKYAAYLANLFRPYGIRLFMSINFSSPKNLGGLKNSDPLDSATINWWS
                     NKAKEIYTQIPDFGGFLVKANSEGLPGPQQYGRTHADGANMLAKCLEPYGGIVIWRAF
                     VYEATGTDRAMQAYNEFMPLDGKFRPNVIVQIKNGPIDFQPREPFHPLFGALKNTKMA
                     LELQITQEYLGFSDHLVYLAPLYSEVLNANTYASNANSLVADIISKKSPGNDITVMAG
                     VANIGNDTNWCGHHFAQANWYAFGRLAWDPYETSENIANDWVKLTFGNNPQIDTPIKQ
                     MMMASREACIEYMTPMGLHHLMGYDHHHGPQPWLDTAGRADWNSIYYHKADKYGIGFD
                     RSTNGSGAVLQYNKPLSDIFNNNKTCPLNLLLWFHHVGWKDTLSTGRILWDELCFQYN
                     DGVEKVKEMQKTWISLSSNIDKERYTEVRNKLDLQLKEATWWRNACLLYFQTFSNMPF
                     PSYLDLKGESLSKYQEKHYKP"
     gene            complement(2800..3264)
                     /locus_tag="PK252_13585"
     CDS             complement(2800..3264)
                     /locus_tag="PK252_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-dependent phosphatase-1"
                     /protein_id="HOT15785.1"
                     /translation="MKPSLFVFDLDFTLWNAGGTWCDCTTPPYKLENGIIRDSYGLKI
                     SLYPDVLEIINELKRQNCQLALASRTEEPSWAVQLLELFNIRHLFEFEEIYPTRKTVH
                     FNSIQAKSGVPYNKMVFFDDEYRNIEDVSKLGVRCVYVNRGIEKYMFREYLD"
     gene            complement(3282..5069)
                     /gene="lepA"
                     /locus_tag="PK252_13590"
     CDS             complement(3282..5069)
                     /gene="lepA"
                     /locus_tag="PK252_13590"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT15786.1"
                     /translation="MVQIRNFCIIAHIDHGKSTLADRLIQATHTLNEREFQDQVLDSM
                     DLERERGITIKSHAIRMDYTFEGQKYALNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ASQGIQAQTIANLYMALEHNLEIIPVVNKIDMDAAKPEEVRDDIVNLLGCKPEDVIFA
                     SAKTGQGIDDILSAIIKRVPAPKGDPNAPLQALIFDSVFNSFRGIVAYFKIVNGTINT
                     GDFVKFFNTGKEYYADEIGVQKLKYEPCQTLSAGAVGYIISGIKVSREVKVGDTITHV
                     QNPCKTAISGFEDVKPMVYAGVYPVDADDYEDLRSSLEKLQLNDASLSFTPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFNMDVITTVPNVSYKVYTTKGDVIEVHNPSGLPAV
                     TLIDHIDEPYIFAQVITKVEFLGAIMKLCLDKRGILKNQHFTTHDRVELNFEMPLSEI
                     VFDFYDKLKSISKGYASFDYHPSDYKLANLVKLDILLNGDSVDALSSLIHRDHSYDFG
                     RRMCEKLKELIPRQQFDIAIQAAIGAKIIARETVKALRKDVTAKCYGGDITRKRKLLE
                     KQKEGKKRMRQVGNVEIPQQAFLAVLKLD"
     gene            complement(5091..5471)
                     /gene="gcvH"
                     /locus_tag="PK252_13595"
     CDS             complement(5091..5471)
                     /gene="gcvH"
                     /locus_tag="PK252_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT15787.1"
                     /translation="MNVPANLKYTKDHEWISVEGNVATIGITDYAQGELGDIVFIEIE
                     TVGKNLKQEEVFGTVEAVKTVSDLFMPASAKIDSKNPRLESEPELVNKDPYGDGWLIK
                     ATISNPAELNSLLDAEAYKALIGA"
     gene            5646..6899
                     /locus_tag="PK252_13600"
     CDS             5646..6899
                     /locus_tag="PK252_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT15788.1"
                     /translation="MKTKIMSLIVGCICFTTACSDDNTENQVNDIKSQLKSAQLVEAS
                     NEFGINLFKETNKLAPDKNLFVSPFSALQALSMTYNGANGITKSEMATTLGFKDASEA
                     QVNEYNQSLAKALLNADAKVMFEIANSIWYRQEYNVLPAFIEINQNSYNAEVKPCNFS
                     DPSTLNAINDWCSIKTHEKIPTILDQIPDDAFMYLINAIYFKGSWQFSFDKSKTQTAD
                     FYLNHDAKVDHSQMMLQTQLNYSSNNTFSAIELPYGNGTFNFVILLPAYDKTVDDIIN
                     GLSSQQWKETISSMTKSDVVVKMPKFKLECEGLLNNPLKSMGMTTAFTGAADFSKITT
                     DAGLFISRVIHKTFIDLNEEGTEAAAVTAVEMKFTSTGGETNTTKWFIANQPFVYAIT
                     EKSTGAILFIGKMLNPTEEKTKFTE"
     gene            7041..7598
                     /locus_tag="PK252_13605"
     CDS             7041..7598
                     /locus_tag="PK252_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15789.1"
                     /translation="MEVKGTSVRSIPEFVDKHFPLRKQEWLNALPEESRQIMSGFIFT
                     NNWYPLKAALTYPMKTISAVFYNGDDVLTAREMGRYSADVALSGVYKFFIQFGSPKYI
                     IERASRIFTTYFQPSEMVVLNVQKNGLKIHLTRFPEPDLIVEENIAGWMERALEKSGC
                     KNVRVKVTQSLARGNKITEFSLSWD"
     gene            7721..8698
                     /locus_tag="PK252_13610"
     CDS             7721..8698
                     /locus_tag="PK252_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOT15790.1"
                     /translation="MKSVLGIGNALVDVLVQLKDDSFLEANNLPKGSMQLVDKPAVDN
                     LIKQIAHAGAKQTSGGSAANTMHGLAGLGCKSGYIGKVGNDYRGNLFESDLRQSKIIP
                     TLLKSATDTGVALSLISKDSERTFATFLGAAVELSAEELLPKYFDGYDYLHLEGYLIF
                     NKALVEKALEIAKSKNMKVSLDLASYNVVEANRDYLLGLIEKSVDIVFANEMEAKALT
                     GLEPEQALEKISSICDIAIVKIGSQGSLIKSGEKSFKAEPIVAKAIDTTGAGDLYASG
                     FLNGLCCEWPLEKCASLASFTAGKVVESIGAKISFTGWDELAHFMLSLR"
     gene            8695..>9080
                     /locus_tag="PK252_13615"
     CDS             8695..>9080
                     /locus_tag="PK252_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15791.1"
                     /translation="MTTFQISSLPIELVHFVLVLAFSLIIGLSQRHLHQTEGDENKLF
                     GSDRTFTFIGLFGYILLYADKQNKYLFITGAIVLTIFLSVYYFNKIKTLGDFGITTIL
                     TALLTYCLPLILLTQPFWIFILILVT"
BASE COUNT         2919 a         1834 c         1690 g         2637 t
ORIGIN      
        1 ggtggcaact gttgcatcaa aaaacgaatt taggtacaga atacgataat cattatgtaa
       61 ttttacatta gtaaatgaaa attctcccaa atgcaaaatg attttttaga aatagaagga
      121 actaaaacta tgcccgccat ttcatttaaa gaagggaaac tggatattat tggacgttca
      181 atccctattg actcatgcga atggtttgcc ccccttctga atgttatgta tgcttataaa
      241 gccaaaaaag gtgaaaaact cgaaataaac attcaacttg actttttaaa cagcgaatca
      301 aaccgcacat tgctcaattt gcttactata gccgaaaaaa tttacaatga agggcacgct
      361 gtacatgtaa aatggtatta taagcccaat gaccctgtga tgttcgagca gggcaatatt
      421 tttcagtcac ttgtagagct tccttttaca tttgaaccta tagtatagcc atcctacaac
      481 actgcaaaag ctgtcttctt gcggtataaa atctgccttt taaatatttt agcagctatt
      541 ttcttctgaa gtttgctatt tttatttttc aaaatttaat tataaaaaaa atgaaaaagc
      601 atgctctaat cttattttgc gcactttctg taaatgctat tgcagaaaac ggatacaacc
      661 tgtggttgag atataagcct gtcaaccacg gtgctgtaaa aaaggaatat cttagtagcc
      721 tttcgtcaat tgaagtaaaa acagaacaag agaataaaca agtcattgaa aatgagttaa
      781 acactgccat taaaagcatg ctccccgggc gctcaaacag taaaggcaat aaaaagcttt
      841 tcattttaag tgcaaaagaa aatcagcaag tgggtaaaaa tttcaaaaca tacctctctt
      901 cattaggcaa tgaaggatac tgcatcattg atacaacaat aaacaatcaa caagctattt
      961 acatcattgc caacaccgga aaaggttgtg tttatggcac atttaaattg ttaagcctga
     1021 tgcaggaaga aaaaagcctc aaaaacattc gcatacaatc tgtccctaaa attaaatacc
     1081 gcatgctcaa ccactgggac aaccccgatg gcagtgttga acgtggctat gcaggaaaat
     1141 ccctgtggta tggcaaaaca aaagtatctg aacaacgatt gatagattat gccagggcta
     1201 atgcctctgt aggtattaac agcgttgtta tcaacaatgt caatgcatct ccggccatga
     1261 tgaacgaaga gaatattaaa tatgctgcat atttggctaa tcttttcagg ccttatggca
     1321 tccgcttgtt tatgtctatc aacttttcat ctcccaaaaa ccttggaggg ttgaaaaact
     1381 cagatccttt agatagtgcc actatcaatt ggtggagcaa taaagcaaaa gagatttaca
     1441 ctcagatacc agactttgga ggctttttgg taaaagcaaa ttccgaaggc cttccaggtc
     1501 ctcaacaata tggacgtacc catgccgatg gtgccaatat gcttgccaaa tgtcttgagc
     1561 cttatggcgg aattgttatc tggagggcat ttgtttatga agcaaccggt acagaccgtg
     1621 ctatgcaagc atataacgaa ttcatgcccc tagatggtaa attccgacca aatgtaattg
     1681 tacaaattaa aaacgggccc attgattttc aacctcgcga accattccat cctttgtttg
     1741 gtgcattaaa aaacacaaaa atggccttgg aattacaaat tacccaggaa tatctgggct
     1801 tctctgacca tttggtttac ctggccccgc tttactcgga agttttgaat gccaatactt
     1861 atgcatcaaa tgccaattca ttagttgctg atattattag taaaaaatcc cctggaaatg
     1921 atattactgt aatggcaggg gtagccaata ttggtaacga caccaactgg tgcgggcacc
     1981 attttgcaca agccaactgg tatgcctttg ggcgcttagc atgggaccct tacgaaacct
     2041 cggaaaacat tgcaaatgat tgggtaaaac taacttttgg caacaatcca caaattgaca
     2101 ccccaattaa gcaaatgatg atggcttcgc gcgaagcctg tatagaatat atgaccccaa
     2161 tgggtttaca tcacctcatg ggttatgacc accaccatgg gcctcaacct tggctcgata
     2221 cagccgggcg tgcagactgg aactccatat attaccacaa agccgacaag tatggcattg
     2281 gttttgaccg ttctactaat ggttctggag ctgtattgca atacaacaag cctctttcgg
     2341 acatctttaa taataataag acttgtccac tcaatctctt actatggttc catcatgttg
     2401 gctggaaaga taccctttct accgggcgta tcctgtggga tgaattgtgt tttcagtata
     2461 atgatggagt agaaaaagta aaagaaatgc aaaaaacctg gattagtctt tcttcaaaca
     2521 tagataaaga aagatacacc gaagtgcgta ataagcttga tttgcaacta aaagaagcta
     2581 cctggtggag aaatgcatgt ttgctctatt ttcaaacatt ctccaatatg ccatttcctt
     2641 cttaccttga tttaaaagga gaaagcctgt caaaatacca ggaaaaacat tataaacctt
     2701 aataacacgt aaaccgcaaa gaataaacac aaaggactgc aaagttgatt actttacttt
     2761 gcagtccttt gtgttaaaaa aataattaat aagaaaagtt caatccaggt attcccgaaa
     2821 catgtatttt tcaatacccc tatttacata cacacaacgg accccaagct ttgaaacatc
     2881 ttcaatattg cgatattcat catcaaaaaa taccatttta ttatatggaa cgccagattt
     2941 agcttgaata ctgttgaaat gaactgtttt tcgagtagga taaatttctt caaattcaaa
     3001 taaatgacgg atattaaaca gttctaacaa ctgaacagcc catgatggtt cttctgtgcg
     3061 tgaagctaaa gccaactggc agttttgacg ctttaattca ttgataatct ccaaaacatc
     3121 tggatatagc gaaatcttca gcccatatga atctcggata atgccatttt caagcttata
     3181 aggaggtgta gtacaatcac accacgttcc gccagcattc cacaaagtaa aatcgaggtc
     3241 gaaaacaaat aacgaaggtt tcacaatatt ttgtggataa atcagtcgag cttaagaacc
     3301 gccaggaacg cctgttgtgg aatttccaca ttgccaacct gcctcatacg cttcttccct
     3361 tctttttgtt tttccagaag cttacgttta cgggtaatat caccaccata acacttggca
     3421 gtaacgtctt ttcttagagc tttgaccgtt tcacgggcaa taatcttagc cccaattgct
     3481 gcctggatgg caatgtcaaa ttgttgcctc gggataagct ctttcagctt ttcgcacatg
     3541 cggcgtccga agtcgtatga atggtcacgg tgaatcagtg aagaaagggc atctaccgaa
     3601 tcgccattaa gcaaaatatc aagctttacg aggtttgcca gtttataatc cgatggatgg
     3661 tagtcaaacg aggcatatcc tttcgaaata ctttttaact tatcgtaaaa gtcaaataca
     3721 atttccgaaa gtggcatttc gaaattcaac tccacacggt cgtgcgtagt gaagtgctga
     3781 tttttcagga taccgcgctt gtccaagcaa agtttcataa tagctccaag aaactctacc
     3841 ttagtaataa cctgggcaaa aatatacggc tcatcaatat ggtcaataag tgtaacagcc
     3901 ggcaaccctg aagggttatg cacctcaata acatcacctt ttgtggtgta aaccttatac
     3961 gaaacgttag ggactgtggt aattacatcc atgttgaact cacggtcgag gcgttcctgg
     4021 acaatttcca tgtgcaacaa gccaagaaaa ccgcaacgaa aaccaaagcc aagtgctgcc
     4081 gaagattctg gggtaaatga aagtgaagca tcattcaatt gaagcttttc taaagaagaa
     4141 cgcaaatctt cataatcgtc agcatctaca gggtaaacac cggcataaac cataggttta
     4201 acatcttcaa aacccgagat agcagtttta catgggtttt gaacatgagt aatagtatcg
     4261 cccactttaa cttcgcggga tactttgatg cccgaaatga tatagcctac cgcacctgcc
     4321 gaaagggttt ggcatggctc atattttaac ttctgaacgc ctatttcatc agcataatat
     4381 tctttgccgg tattgaaaaa tttaacaaaa tcgccggtat tgatagttcc attgacaatt
     4441 ttgaaataag ccacaatgcc acggaatgaa ttgaaaaccg aatcaaaaat caaggcttgc
     4501 aaaggcgcat taggatcgcc ttttggcgct ggcacccttt taataatagc agacagaata
     4561 tcatcaatac cttgcccggt tttggcgctg gcaaatataa catcttcagg tttgcaacca
     4621 agcaaattta cgatgtcatc gcgtacttcc tcaggttttg ccgcatccat atctatttta
     4681 ttgaccacag gaatgatttc gaggttatgc tccaatgcca tgtataggtt ggcaatagtt
     4741 tgagcctgaa taccctgcga agcatcaact ataagcaacg ccccttcgca agcagcaata
     4801 gaacgcgata cttcataaga aaagtcaaca tgccccggcg tatcaataag gtttaaagca
     4861 tatttttgcc cctcaaatgt ataatccatg cgtattgcat ggcttttgat ggtaatgcca
     4921 cgttcacgtt caagatccat gctatccaac acctgatcct gaaattcacg ttcatttaat
     4981 gtatgagtag cctgaataag cctgtcggcg agggtacttt ttccgtgatc gatatgagca
     5041 atgatacaga aattgcgaat ttgcaccatt tatatttttc agtgtttgaa ttatgcacca
     5101 attaaagcct tataagcttc tgcgtcaagc aggctattga gctctgcagg atttgaaatt
     5161 gtagccttga ttaaccaacc atcgccgtat gggtctttgt ttaccaactc aggttctgac
     5221 tccaaacgtg ggtttttact atcaatcttg gcagaagcag gcatgaacaa atctgaaaca
     5281 gtttttactg cttcaacagt accaaaaact tcttcctgct tcaggttttt tccaactgtt
     5341 tctatttcaa taaaaacgat atctcccaat tcgccctggg cgtaatcggt aataccaata
     5401 gtggcaacat tgccttctac actgatccat tcgtgatctt ttgtgtactt cagatttgca
     5461 ggaacgttca taagtatttt ggtttttaca tgtttgaccg caaaattaag caattaatta
     5521 cgatttacaa attttagaaa aatgaaccaa aagacgtttt aataccctta cgcaaccttt
     5581 ttaaaagtct gccatctaac taaaaattct gcagaaaata tttacttact taaattttaa
     5641 aaaacatgaa aacaaaaata atgtctctta tagttggttg catctgtttt acaactgcct
     5701 gctctgatga caataccgaa aatcaggtaa atgacataaa atcgcaactg aaatcagccc
     5761 agttggttga ggcttcaaat gaatttggga ttaatctgtt taaggaaaca aataaacttg
     5821 ctccagacaa gaacctgttt gtatcgccat tcagtgcatt acaggcgctg tccatgactt
     5881 acaatggggc caatggcatt accaagtctg aaatggctac aactttaggc ttcaaagatg
     5941 cttccgaagc ccaggtgaat gagtacaatc aatcacttgc aaaagccttg ctaaatgcag
     6001 acgcaaaagt gatgtttgaa atagccaatt ccatttggta ccggcaagag tacaacgtat
     6061 tgccagcatt tattgaaatt aaccaaaatt catacaatgc agaggttaaa ccatgcaact
     6121 tctccgatcc ttcaaccctg aatgccataa atgactggtg tagcatcaaa acacacgaaa
     6181 aaatacccac catattagac cagataccag acgatgcttt tatgtacctt atcaatgcta
     6241 tatatttcaa aggctcctgg caattttcat ttgacaaatc aaagacccag acggctgatt
     6301 tttatttgaa ccacgatgct aaagttgacc attcgcagat gatgctccaa acacaattaa
     6361 actactcaag taacaatacc ttcagcgcca tagaattgcc ttatggaaac ggcactttta
     6421 attttgtaat cctccttccc gcttatgaca agactgttga tgatattatc aatggcttat
     6481 cctctcaaca atggaaagaa acaatttcga gcatgacaaa aagtgatgtt gtagtaaaaa
     6541 tgccaaagtt caagctcgaa tgcgaaggtt tgctgaataa ccctctgaaa agcatgggca
     6601 tgactactgc tttcactggt gcagcagact tttcaaaaat aacaacagat gcaggcctgt
     6661 ttatttcgcg cgttatccat aaaacattta tcgatttaaa tgaagaaggc actgaagctg
     6721 ctgctgttac tgccgttgaa atgaaattca cttcaaccgg aggtgaaacc aataccacaa
     6781 agtggtttat tgccaaccaa ccttttgtct atgctattac cgaaaaatca accggtgcca
     6841 ttttgtttat aggaaaaatg ttaaatccga ccgaagaaaa aacaaagttc acagaatagc
     6901 aaatatctgt agagatagca ttttaagcta ttttcaagct taaaatgcta ttttggcttc
     6961 tataaaaatt aaaattgatt tgtcctgttt catgtaaatg ttataacttt ataatgaaaa
     7021 gatatgcaga tatataaatt atggaagtta aaggcacatc agtcaggtca atccccgaat
     7081 ttgttgacaa acattttcca cttcgcaagc aagaatggct caatgctttg cctgaagaat
     7141 caaggcaaat catgagtggc tttattttta ccaataactg gtatcccttg aaagctgctc
     7201 ttacttatcc tatgaaaact attagcgctg ttttttataa tggagatgac gtgctaacag
     7261 cccgtgaaat gggccgttat agcgctgatg ttgcactttc aggagtttat aaattcttca
     7321 ttcagtttgg ttcgcccaaa tacattattg aaagggcaag caggattttt acaacctatt
     7381 ttcaaccctc agagatggtt gtactaaatg ttcaaaaaaa cgggctgaag atacatctta
     7441 cccgtttccc cgaacccgat ttaattgttg aagagaatat tgcaggctgg atggaacgtg
     7501 ccctcgagaa atcaggatgc aaaaatgtga gggttaaagt aacgcaatcg ctggcacgcg
     7561 gcaataaaat aaccgaattt agtttaagtt gggattaatg caaacctctg aatacttctc
     7621 aggggttttt cttttgcttt gccagataaa atagaaaacc acaggaaaat aattcaatta
     7681 tgaatttggg gttatatttg catcaaaaca taaacacaaa atgaaatcag tattaggtat
     7741 aggcaatgca ttggtagatg tattagttca gttgaaagat gattcgttcc ttgaagcaaa
     7801 caacttgccc aaagggtcaa tgcaattagt agataaacca gctgttgaca atttgattaa
     7861 gcaaattgcc cacgccgggg ctaaacaaac atcgggaggc tcggctgcaa atactatgca
     7921 tggtttagct ggcctaggtt gtaaaagtgg ttatattggg aaggtaggaa acgactaccg
     7981 tggcaacctt tttgaaagtg acctgcgtca aagcaaaatc attccaacac ttttaaaatc
     8041 ggctacagat acaggtgttg cattatcgtt gataagtaaa gactctgaac gtacttttgc
     8101 cacatttctt ggggctgcag tagaattatc tgctgaggag cttttaccta aatactttga
     8161 tggttatgat tacctgcatc ttgaaggcta tcttattttt aacaaggctt tagttgaaaa
     8221 ggctttagaa atagccaaat ctaaaaatat gaaggtttct ctcgacctgg caagctacaa
     8281 tgtagttgaa gccaacaggg attatcttct tggtttgatt gaaaaatcag tagatatagt
     8341 atttgccaat gaaatggaag ccaaagccct caccggactt gaaccagagc aagctctcga
     8401 aaaaatatcc agcatttgtg acattgctat tgttaagatt ggctcacaag gttcgttaat
     8461 taaaagtggc gaaaaatcct tcaaagcaga gcctattgtt gcaaaagcca ttgatactac
     8521 cggcgctggc gacttatacg cctcaggctt cctaaacggc ctgtgctgtg aatggcctct
     8581 cgaaaaatgc gcaagtttag cttcatttac tgccggaaag gtagtagaat caattggggc
     8641 aaaaatcagc tttaccggtt gggatgaatt ggcccatttt atgctttctt taagatgaca
     8701 acgtttcaaa tatcgtcatt acctatagaa ctggtacact ttgtactggt attggccttt
     8761 tcattgataa tagggctttc gcaaaggcac ttgcatcaaa ccgaaggtga tgaaaacaag
     8821 ctcttcggct ctgaccgtac ttttactttc attgggctgt ttggctatat tctgctatat
     8881 gccgataaac aaaataaata cctgtttatt accggggcta tagtattgac aatatttctg
     8941 agcgtttact attttaataa aatcaaaacg ttgggagatt ttggaattac taccattctt
     9001 actgcccttc tgacgtattg cctcccgctc atcttgttga ctcaaccatt ctggattttc
     9061 atattaatac ttgttacaat
//
LOCUS       DAOSPF010000102         9061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7636_length_9061_cov_6.205752, whole genome shotgun sequence.
ACCESSION   DAOSPF010000102 DAOSPF010000000
VERSION     DAOSPF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7636_length_9061_cov_6.205752"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK252_13620"
     CDS             complement(<1..277)
                     /locus_tag="PK252_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15792.1"
                     /translation="MKRACLEVNNNTHEYRLVGTSCIRPLEINFVRIKKLLIIKLGLL
                     AALLSTQLNGIGQATANKNIIGTYSKPDCPEWVNNAVFYQIYPQTYYD"
     gene            complement(462..3155)
                     /locus_tag="PK252_13625"
     CDS             complement(462..3155)
                     /locus_tag="PK252_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15793.1"
                     /translation="MRKLYALLLEDVQKDVELLTEILTGEGFELEVDVVEAEQDFVQN
                     LKRHNYDIIFADFTLPSFNGQEALVLAKKICPNVPFICISGTIGEDRAVELLKQGATD
                     YVLKDRMERLPFATKRALDAALQLQKFRQAEIELQTNRKLLQTIINNALDLIYIKDIN
                     GNYILLNHAAEKAYGKNANDVVGKDDFSVLSKSDAKTIMEVDKEVIAKAKSIEIEENI
                     TLADGLIHTFHTIKSPMFDEQGKITGLFGIARDVTECKKAEKILHETQEMFKLIHDSS
                     IDAILLTCPDGRILSANPSACRMFGRSEEEICRLGREGLVDTSDPKIQAAIEERARTG
                     WFKGELTFIRKDNTKFPADVTSAIFKDKEGQEKTSMIIRDITEQKKAEEAINLERKLL
                     RTLINNLSDAIYVKDNKGRKLIANTADLKIMNCSDESEVIGKTDRDIFPNSRGERGYN
                     EDMEVILTGNAIINKEDSYIDAEGKEHWRLTTKIPLFNEQKQIVGLVVFGHDITERKK
                     FEEQLLIAKDKAEASDRLKTAFMQNISHEVRTPLNGILGFAELIANPNIETERKAEFY
                     NILSASSNRLLNTITDYMDMSLIVSNNVSVQYESIKFSDVLSQLNKDYQNTCISKNLN
                     FNIRYDEKTKNAEFRSDPTLLAKIINHMVDNAIKFTKEGSIFINFNIDQSQLEFTIKD
                     TGIGISKEAQEKIFDVYTQESTSTTRGYEGSGLGLSIVNGFLKILGGKIRLNSEKGAG
                     AEFYITIPINNENKPDKITDKNSCSSTTENNKLPVILIVEDDFVNYTLFEAILEPHAS
                     QILYARNGLEAIEICQLHPEISIVLMDVKMPVMNGLEATKEIKNFRKDLPVIALTAYA
                     MSGDEYKMFEAGCDAYIPKPAKAKDILEKIASLGFNIQTTT"
     gene            complement(3180..3629)
                     /locus_tag="PK252_13630"
     CDS             complement(3180..3629)
                     /locus_tag="PK252_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15794.1"
                     /translation="MVELRTILLAEDNPKDVELTMEALEDSLLANRIIVVKDGIETLE
                     YLRCEGQYANREPGLPSVLLLDIKMPRMDGIEVLREIRNDPRLKLLPVVMLTSSREDK
                     DLLNSYYLGVNAYVVKPVNFTGFIDAVKQLGIFWGLVNELPPEIKNS"
     gene            complement(3641..5476)
                     /locus_tag="PK252_13635"
     CDS             complement(3641..5476)
                     /locus_tag="PK252_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15795.1"
                     /translation="MKAFRNMSIKLKLIIVIIGTSIITLFVAFTTYLGIDIYREFNEM
                     KKNAVLQASLIGQYAAAPLLFGYKEETTEVLNKLTAIPSIKDACLYSPNSGEVFVTYH
                     KTPDSSFVFPVLHNDNASFQNGYFHIFYSIKYDNNYCGSLYLRIDPEDIYTKIEKSVL
                     LMFVLLLVLIIVVYFISLKLQKIISLPILKLAEQTAIISQNHDFSVRLTPYGNDEIGE
                     LYNQFNNLLLQILIKQKERDKVLEDLQESETHFRFLFEQNPAMMLIYDLSSLKIISVN
                     EAFIQHYGYQQDEILNMKLTDLYPKNEKEAIIQLVKTLKGLAYTGEWHHVKKDGTIIN
                     IETHSHSYIAEDNAVRIVVINDITARKKAEEEILTLNAELENRVKERTAQLEATNKEL
                     EAFSYSVSHDLRAPLRHVNGYLELLSKRGIQHLDEKSKHYMQSISEAATHMGILIDDL
                     LNFSRAGRMELKLDKIDMNRPIKDALNILEQDTKNRKIEWKITQMPEIMADYAIMRQV
                     WTNLIGNAVKYTKKRELALIEVGYTEDAQEYIFYVRDNGAGFDMNYAQKLFGVFQRLH
                     STEDFEGTGIGLANVRQFIKKHNGRTWAEGEIDKGATFYFSLPKI"
     gene            complement(5473..5973)
                     /locus_tag="PK252_13640"
     CDS             complement(5473..5973)
                     /locus_tag="PK252_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOT15796.1"
                     /translation="MVFIHNNLSPQSDINTLKAVAIEKIALFITWPPGSLSNQEFTIG
                     ILGDNPITETLKQIYLEKKIKDLRVNIVEIKDIESITSSCQLLFIPKIKMAELNKLLK
                     HTSNKPILTISDTKGFAEAGCMINFYVYENKLRFELNQKGMDEAGFKIDYRLLKVSKI
                     INPVIE"
     gene            complement(6054..8114)
                     /locus_tag="PK252_13645"
     CDS             complement(6054..8114)
                     /locus_tag="PK252_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15797.1"
                     /translation="MKKLKALILLVSCINIYGYVHAQDSAANKTAEELFSLSLDDLLN
                     VVITPSKLPQHEGSYTQKVDLIKSEQIESIVQGNRNICELISKLPGTSVSVLSRNDAN
                     WGTYGGIGPKYSTYMLQGLPIDAFVDPMSLDNNIIEQIEVQRGPASVIYPNYLSQDFA
                     GSQSPLSGTVNLVLKDKITEQKTVLHSSYGSYNTLNEQVYHQNKKDRLNYFLGSSYEM
                     SDYTNYGIENSWLNMKKSPEYRKTKLYGGITFYPEKNEKQKLSVFCNSTWHSGDVGRV
                     YRQFDNRYSTLNVGYEILLSEKLHFQTHAGIRSYRRMQQESRYGVTDTLNSNNGVNQF
                     IVPVDISIAWMQGSTGSLSIGADYQGAGYETWSDPLKGFQIYGNKSSAYQTGIYLQEE
                     WRPIEKLTLRGGLRYAYIENTIDLVNGNNPIDKRLSWKKLIWSAGTRYSVSKSLAVYA
                     NGGSSFSAPGLKSIGGTILLSDLGVQGHNGQLPNPDIKPENGLGIDIGTDCKCQNTFK
                     INVRGFYYMLRDGIIDNIVSLNPSQTQSINTKSNCKGGEIELLQMVNSTISWYANFTY
                     MQTNIENDLNPNLDQVEIPFSPNYIGNIGATIILPFGLNFSPAINYNGGYYDAISKPD
                     RQWYKPGAVVNTHISQRLAQNNFYTLDCTLQLYNITNNKYKMPWQFQNTGFSGMLGVK
                     VTFK"
     gene            complement(8336..>9061)
                     /gene="arsB"
                     /locus_tag="PK252_13650"
     CDS             complement(8336..>9061)
                     /gene="arsB"
                     /locus_tag="PK252_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOT15798.1"
                     /translation="IDYMYGVILIGLARCIAMVIVWNDLAKGDAEYAAGLVAFNSIFQ
                     VLLFSVYAYVFIAILPGWFGLPVNDSVSQITIGQIAKSVFIYLGIPFIAGFLTRFVFI
                     RVKSKEWYQSKFVPKISPLTLIALLFTIVVMFSLKGEYIVKIPLDVIRIAIPLVIYFV
                     VMFVVSFFMSKKTGATYGQATTLSFTAASNNFELAIAVAIAIFGINSGEAFAAVIGPL
                     VEVPVMIGLVNVAFWFKKKYFAS"
BASE COUNT         2400 a         1743 c         1796 g         3122 t
ORIGIN      
        1 agtcgtaata tgtttgaggg tatatttgat agaatactgc gttattgacc cattcagggc
       61 agtcgggctt ggagtatgta ccgataatgt ttttgtttgc tgttgcctgg ccaatgccat
      121 taagttgtgt tgataaaaga gctgccaata agcccagttt gattatcaac aattttttta
      181 tccgaacaaa attaatttct aaagggcgga tacaacttgt gccgactaat cggtattcat
      241 gcgtgttgtt gtttacttct aaacatgctc gtttcataaa tgtaaaatgg ttaatttgtt
      301 atcaaaaaaa atatgagccg aaaatactac tgttcttttt ttttcagctg cttgatatgt
      361 tgcattccga tagacaaaat gttaaagcag gtgtttagtg tgcccatatt tatttgtttt
      421 gagtagaaag aaccctggct atgctttaca atcagaaaat tttatgttgt tgtttgaatg
      481 ttgaacccca ggctggctat tttttcaaga atatcttttg ctttggctgg tttagggata
      541 tatgcgtcac aaccggcttc gaacatttta tattcgtcac cgctcatggc ataggctgtg
      601 agggctatta ccggcaaatc tttcctgaaa ttttttattt ctttggtagc ttcaagcccg
      661 ttcattactg gcattttaac atccatcagg acaattgata tttcggggtg caattggcaa
      721 atctcgatgg cttcaagtcc gttgcgtgcg tataaaattt gcgaggcatg tggttctaat
      781 atggcctcaa ataaagtgta atttacaaaa tcatcttcta ctattaatat aactggcaac
      841 ttgttgtttt cagttgtgga agagcagcta tttttatcgg taattttatc gggtttattt
      901 tcattgttga ttggtatagt gatgtaaaat tcggctccgg cccctttttc tgaattcagg
      961 cggattttac ctcccaggat tttcaaaaag ccatttacaa tggacaaccc cagcccacta
     1021 ccttcatatc ctcttgtggt agaagtgctt tcttgcgtgt aaacatcaaa tatcttttct
     1081 tgagcttctt ttgatatacc aatgcctgta tctttgatag taaattctaa ttgagattgg
     1141 tcgatgttaa aatttataaa aatggaacct tctttggtaa atttaatggc gttgtccacc
     1201 atgtggttga tgattttagc caatagggtt gggtctgatc taaattcagc attttttgtt
     1261 ttttcatcat aacggatgtt aaaattgagg ttttttgaga tgcaggtgtt ttgatagtct
     1321 ttgtttaatt gagataaaac atcggaaaat tttatgcttt catattgtac gctaacattg
     1381 tttgatacta taagagacat atccatataa tcggtaatag tatttaataa acgattactg
     1441 gaggccgata aaatgttata aaactcagct tttctttctg tttcaatatt ggggtttgca
     1501 attaattcgg caaaaccgag tatgccattt agcggggtgc gtacttcatg cgagatgttt
     1561 tgcataaaag ctgtttttag cctgtcgctg gcttctgctt tatctttggc aataagcagc
     1621 tgttcttcaa atttttttct ttcggttata tcgtgcccaa aaacaacaag tccaacaatt
     1681 tgtttttgtt cattaaaaag ggggattttg gtggttaatc tccaatgttc tttaccttca
     1741 gcatcaatat aagagtcttc tttattaatt atagcattgc cagtaaggat tacttccatg
     1801 tcttcgttat accctctttc gccccggctg tttggaaaaa tgtcacgatc ggtttttccg
     1861 attacttctg attcatctga gcagttcata atcttcaaat cggctgtatt agcaattagc
     1921 ttgcggcctt tattgtcttt aacataaatg gcatcggaca ggttgttgat aagggtcctg
     1981 agtagtttgc gttcgaggtt tatggcttct tcggcttttt tttgttcagt aatgtccctg
     2041 ataatcatac tggttttttc ctggccttct ttatctttaa aaatggcaga tgtcacgtcg
     2101 gccgggaatt tggtattatc ttttctgata aatgtcagct cgcctttgaa ccagcctgtt
     2161 cttgcccttt cttctattgc agcctgaatt ttcgggtcag aggtgtcaac cagcccttct
     2221 cttcccaagc ggcatatttc ttcttcgctt cgcccaaaca tcctgcaggc tgatgggttg
     2281 gcagataaaa tcctgccgtc aggacaggtt aataaaatag catcaatgct tgaatcgtgt
     2341 atgagtttga acatctcttg tgtttcgtgc aaaatttttt cagctttttt gcattcggta
     2401 acatcacgtg caataccaaa taacccggtt attttgcctt gttcgtcaaa catggggctt
     2461 tttatggtat gaaatgtatg gataaggcca tcggctaaag tgatattttc ttctatttct
     2521 attgattttg ctttagcaat gacttctttg tcaacctcca tgattgtttt ggcatcgctt
     2581 tttgaaagta cactaaagtc atctttccca acaacatcgt ttgcgttttt gccataagct
     2641 ttttctgctg catggtttaa aaggatataa tttccgttga tatctttaat gtaaattaaa
     2701 tccagggcat tgttgataat ggtttgcaat agcttacggt tggtttgaag ctcaatttcc
     2761 gcttgtctaa acttttgtag ttgtaatgcg gcatctaatg cgcgtttagt ggcaaaaggc
     2821 aaacgttcca tacggtcttt gagaacataa tctgtagccc cttgtttaag aagctctaca
     2881 gctctgtctt ctccgatagt tcctgatatg caaatgaaag gaacgtttgg gcatatcttt
     2941 ttggccaata ccaatgcttc ttgaccatta aagcttggca aggtaaagtc ggcaaatatg
     3001 atatcgtaat tgtggcgttt taagttttgc acaaagtctt gttcggcctc aaccacatca
     3061 acttctaact caaatccttc tccggtaagt atttctgtca gaagttcaac atctttctga
     3121 acgtcttcga gaagtaatgc atacagtttt ctcataacca cagttttgaa ttgtttacat
     3181 caggaatttt tgatttccgg gggaagttca tttaccaatc cccaaaaaat acccagttgc
     3241 tttacagcat ctatgaaacc agtaaaattg acaggtttga ccacataagc atttactccc
     3301 aggtagtagc tgttgagcaa gtccttatct tcgcgtgatg aagtaagcat gactacaggc
     3361 agtaatttaa gcctggggtc attgcgtatt tcgcgcagca cttcaatgcc atccattcgg
     3421 ggcattttaa tatcaagcaa taacactgag ggtaagccag gctccctgtt ggcatattgg
     3481 ccttcacatc gcaggtattc aagtgtttct atgccatctt tcactacaat gatcctgttt
     3541 gccaatagac tatcctccaa agcttccatg gtgagctcta catctttggg attgtcttcg
     3601 gccaataaaa tagttcttag ttctaccata tttttatctc ctatattttg ggtaatgaaa
     3661 aataaaatgt tgcccctttg tcaatttcgc cttcggccca agtgcggcca ttgtgttttt
     3721 ttataaactg gcggacgttg gctaacccaa tgcctgtgcc ttcaaagtct tcggttgaat
     3781 gtaacctttg gaatacgcca aacagctttt gggcataatt catatcaaag cctgcgccat
     3841 tgtcccggac ataaaatata tactcttgtg catcttcagt atatccaacc tcaatcaatg
     3901 ccaattcgcg tttttttgtg tatttaacgg cattgccaat aaggtttgtc cacacctgtc
     3961 gcataatagc gtaatcggcc attatttctg gcatttgggt tattttccac tcaattttcc
     4021 ggtttttggt atcttgttcc aatatgttca aagcatcttt tatcgggcgg ttcatatcta
     4081 tcttgtcaag tttcagctcc attcgcccgg cccttgaaaa attcaataag tcgtcaatta
     4141 gtattcccat gtgtgttgca gcttctgaga tggattgcat gtaatgtttg ctcttttcgt
     4201 cgagatgctg gatgccacgt ttgctcaaca attcgagata cccattgacg tgccttagcg
     4261 gggcccgcaa gtcgtgagag actgagtaag aaaaggcttc aagctctttg ttagttgcct
     4321 caagctgagc ggtgcgttct ttgaccctgt tttcgagttc tgcattgagg gttaaaattt
     4381 cttcctcggc ctttttgcga gctgtaatat cattaatgac cacaatgcgc acggcattgt
     4441 cttcggcaat ataactatgc gaatgtgttt cgatgttaat aattgtcccg tcttttttta
     4501 catggtgcca ttcacctgta taggccagcc ctttcaatgt tttgactaat tgaatgatgg
     4561 cttctttttc gtttttgggg taaaggtcag tgagcttcat gtttagtatc tcgtcttgct
     4621 ggtatccata atgctggata aaagcttcgt tgaccgaaat aattttcagg ctggacaggt
     4681 cgtatatcaa catcatagcc gggttttgtt caaataagaa ccggaaatgg gtttcacttt
     4741 cctgcaaatc ttctaacact ttatcccttt ctttttgctt aataagtatt tgtaaaagaa
     4801 ggttattgaa ttggttgtaa agttcgccaa tctcgtcatt gccataaggg gtcaggcgta
     4861 ccgaaaaatc atggttttgt gatataatag ctgtttgttc ggctaatttc agtattggta
     4921 gcgatattat tttttgcagt tttaatgaaa taaagtaaac tacaattata agtaccagca
     4981 gcaggacaaa catgagcaaa acgctttttt caatcttagt gtaaatgtct tccgggtcta
     5041 tccgcaggta cagtgaacca cagtaattgt tatcgtattt tattgaataa aaaatgtgaa
     5101 aatatccatt ttgaaaagag gcattgtcat tatgcaaaac aggaaaaaca aaagagctgt
     5161 ctggtgtttt gtgataagtg acaaatactt caccagaatt ggggctgtat aaacaggcat
     5221 ctttgatgga tggtatagcg gtaagtttgt ttaatacctc ggtggtttct tctttatacc
     5281 cgaataataa tggagctgca gcatattgcc ctattaatga ggcctgaagt acggcatttt
     5341 ttttcatttc attgaattct ctgtatatat caatgcccag ataagttgta aatgctacaa
     5401 ataaggtaat gatacttgta ccgataataa caattatcag cttcaatttg atggacatat
     5461 ttctaaaagc cttcattcta ttaccggatt tattattttg gacactttta acaaccgata
     5521 gtcaatttta aaccctgctt catccattcc tttttggttt aactcaaagc ggagtttgtt
     5581 ttcatatacg taaaagttta tcatgcaacc ggcttcggca aaccctttgg tatcggagat
     5641 ggttaaaatt ggcttgttgc tcgtgtgttt caacaattta ttcagctcgg ccatttttat
     5701 ttttgggata aaaagtaact gacaagaaga agtgatgctt tcaatgtcct ttatttcaac
     5761 aatgtttact cttaagtctt taattttctt ctccaggtat atttgtttca atgtctcagt
     5821 aatcgggttg tcccctaata ttccaatggt aaattcctgg tttgataggg agcctggagg
     5881 ccaggttata aataatgcaa ttttttctat agctacagcc ttcagggtgt ttatgtccga
     5941 ttgtggagat aagttattat gtataaatac cataagcagg atgagaacaa ttctgacatt
     6001 tgtaaaacct ttatttttcc tggcccgaca catattgaaa atctccgcct gctttattta
     6061 aaagttactt taacccccaa cattcccgaa aagcctgtgt tttggaattg ccagggcatt
     6121 ttatacttgt tgtttgtaat gttatataac tgtaaagtac aatctaaagt gtaaaagttg
     6181 ttttgggcaa ggcgttggga gatgtgggta ttgacaacag cccctggttt gtaccattgc
     6241 ctgtctggct tggaaatagc gtcgtaatag ccgccgttgt agtttatagc tggcgaaaag
     6301 ttcagcccaa agggaagtat gattgttgcc ccgatatttc caatataatt gggcgaaaaa
     6361 ggtatttcta cctggtctaa attcgggttc aggtcattct ctatgtttgt ttgcatgtag
     6421 gtaaagttgg cgtaccatga gatggtagag tttaccattt gtaaaagttc tatttctccg
     6481 cctttacaat tggacttagt gttgattgac tgtgtttgcg atgggtttag gcttactata
     6541 ttgtctatta tgccgtctct aagcatgtaa tagaacccgc ggacattaat tttaaaggtg
     6601 ttttgacatt tgcaatctgt tccaatgtct atgcccagcc cgttctctgg ctttatatcg
     6661 gggttaggca actggccgtt atggccttgt acgcccaggt cgctaaggag tattgtcccg
     6721 cctattgatt ttaacccggg ggcgctaaag ctgctccctc catttgcata tacagccaat
     6781 gatttgctaa cagagtatct tgtacctgca ctccatatta gctttttcca tgacaaacgc
     6841 ttgtcgatgg ggttgtttcc gttgactaaa tcaatggtat tttcaatata tgcataacgt
     6901 aagcctcctc tgagggttag tttttcaata ggccgccatt cttcctggag gtatatccct
     6961 gtctgatatg ccgaagattt attgccatat atctgaaagc cttttaatgg atcagaccat
     7021 gtttcataac ctgcaccttg gtaatctgct ccaatactta gtgaacctgt acttccctgc
     7081 atccatgcaa tggaaatgtc aacaggcaca ataaactggt tgaccccgtt atttgaattt
     7141 aaggtatctg ttactccata tctgctttcc tgttgcattc gcctgtacga cctgattccg
     7201 gcatgggttt gaaaatgaag tttttcgctt aacaatattt cgtacccaac attcaacgtg
     7261 ctgtaccgat tgtcgaattg gcgatatacc cggcctacat cgccactatg ccaggtgcta
     7321 ttgcaaaaaa cagagagttt ctgcttttca tttttttcgg ggtaaaatgt aatgccgcca
     7381 tatagttttg tttttctgta ttcggggctt tttttcatgt tcagccacga gttttctatt
     7441 ccatagttgg tgtaatccga catttcataa gacgaaccaa gaaaatagtt caggcggtct
     7501 tttttgttct gatgataaac ctgttcgtta agcgtgttgt atgagccata agatgaatgt
     7561 aacacagttt tttgttcggt tattttgtct ttcaacacta aattgacggt gccactcaac
     7621 gggctctgag aacctgcaaa atcttgtgac aggtaatttg gatatataac agacgcgggg
     7681 cctctttgta cttctatttg ctctataata ttgttgtcaa gggacattgg gtcaacgaaa
     7741 gcgtcaatgg gcaggccctg caacatatat gtactgtatt tggggcctat tcccccataa
     7801 gttccccaat tggcatcgtt gcgcgaaagg acactgacag aagtccccgg taattttgaa
     7861 atcaactcgc aaatattcct gttcccctgc actattgact ctatttgctc agattttatc
     7921 aggtcaactt tttgcgtgta gctcccttcg tgttgaggaa gttttgatgg ggtgattaca
     7981 acatttaaaa gatcgtcaag gctaaggctg aatagctctt cagcagtttt atttgctgca
     8041 gagtcttggg catgcacata accgtagatg tttatgcagg ataccaacaa tattagcgct
     8101 ttcagctttt tcatatcttt caatattaag tcctaaaagc ttaatgataa aaactgtctg
     8161 aacaactttt ttaaagggct taggggacat aactttaaat aagatattga aagttaacta
     8221 aaaaattcat tttaaacaac aaagcctgac cattttttta agtggccagg ctttaatatt
     8281 tagcgatatt ttgcgcttgg gtaaaaaagt gtgattttgt tttttaattc attttttaac
     8341 tggcaaaata ttttttctta aaccaaaaag caacattcac caacccaatc atcacgggca
     8401 cttcaaccaa tgggccaatg acagcagcaa aggcttcgcc ggagttgatg ccaaagatgg
     8461 caatggctac agcaatagcc aactcaaagt tgttgctggc agcagtgaac gaaagcgttg
     8521 tagcttgccc gtatgttgcc ccggtttttt tgctcataaa aaacgagacg acaaacatga
     8581 ctacaaagta tattaccagc gggatggcaa tacgtattac gtccagcggg atttttacga
     8641 tgtactcacc tttgagcgaa aacatgacca ctatggtgaa aagcagtgct atcagggtca
     8701 gagggctgat tttgggtaca aatttgctct ggtaccattc ttttgatttt acccgtataa
     8761 aaacaaagcg agttaaaaag cctgctataa aaggtatccc taagtatatg aatacgcttt
     8821 tggctatttg gccaatggtg atttgcgaaa cactatcatt tacaggcaac ccaaaccatc
     8881 cgggaagtat ggcaataaat acataggcat ataccgaaaa tagcagtacc tggaatattg
     8941 aattaaaggc cactaatccg gctgcatatt ctgcatcgcc tttggcaagg tcgttccaaa
     9001 caatgaccat agctatacaa cgtgccagcc ctatgaggat aactccgtac atatagtcga
     9061 t
//
LOCUS       DAOSPF010000103         8921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_7802_length_8921_cov_7.726370, whole genome shotgun sequence.
ACCESSION   DAOSPF010000103 DAOSPF010000000
VERSION     DAOSPF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_8921_cov_7.726370"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..1723
                     /locus_tag="PK252_13655"
     CDS             461..1723
                     /locus_tag="PK252_13655"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT15799.1"
                     /translation="MFEKFKSSQAFDVLRPVNFRYFLLYRFFMTLATLMQSVVVGWHI
                     YKLTGNVFALGLIGLTEVIPQVSVALFAGHFVDICNRQKVIFYTTFLLLAGSAWLVIY
                     SIPAFGLFTRFGVTPIFITIFLTGLSRGILMPAHNALLGQLVERKYFPKAATWSSVTW
                     HIAAVCGPAVGGLVYGFSQNEVVTYSTVFTAYLTAFLLISRIKVEKLQTGEITEGIFA
                     RIHEGVRFVFNSPYLWGAFTLDMFAVLFGGAVALLPAFAKDVLFVGPQSLGFLRAAPA
                     MGAILMSLFLMFRPPVKNTGTLLFIAVSGFGLCMIGFALSTSFALSLILLFLSGIFDN
                     VSVVIRGTILQIYTPEHMKGRVSSVNSIFIGSSNELGAFESGLAARLMGLVPSVVFGG
                     AMTLIVVGVMSKVNPALKKMSFAKQHVQ"
     gene            1787..2680
                     /locus_tag="PK252_13660"
     CDS             1787..2680
                     /locus_tag="PK252_13660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT15800.1"
                     /translation="MFPQYIQPAAFSDYELIDSGNFEKLERFGQYVLARPEPQAIWHK
                     AMTEDEWKNKAHAWFRLNVNAHNKERGEWVLKKGMPQQWQVNYAYKEMKLSFRLGMTS
                     FKHVGLFPEQAENWNFIYENIKSLQIANPEVLNLFAYTGGASLAACAAGAKVTHVDSV
                     KQVVTWSRENMELSGLANIRWIVEDALKFVQRQVRKGAKYQGIILDPPAYGRGADGEK
                     WILDEHLAVMVDCCNKLLDDNGFVVLNLYSMGFSPLVAQNILETSFVGAKNVQSGELY
                     FEDKAKHRLPLGIFARFNKNS"
     gene            2677..3333
                     /locus_tag="PK252_13665"
     CDS             2677..3333
                     /locus_tag="PK252_13665"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT15801.1"
                     /translation="MTEKRKLLLSLPIPLFFIVVIWCVKLVEYANNTSFYFLGVYPHS
                     FKGIIGILTAPLVHDDIKHLVNNSVPFLLFSVATFFFYRPLGLRVLFWVWLMSGLWVW
                     FGARPAYHIGASGLVYGLGAFLFFSGAIRRNAHLAAVSLIVVFLYGSMIWGIFPFVPD
                     ISWEAHLSGGIAGLILAIVYRKDGPQPKKYEWEDEENVDSSDVLIDKLEENNSTPEKN
                     "
     gene            complement(3334..4032)
                     /locus_tag="PK252_13670"
     CDS             complement(3334..4032)
                     /locus_tag="PK252_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOT15802.1"
                     /translation="MDKYFVSEIFRSIQGEGNYAGVNCLFIRFQFCNLTCSWCDTKYT
                     WLRKSGTFKEYSKEELRKVIEDSPCHHIIFTGGEPTLYRLDELAVTGKKFHVETNSSI
                     LPWELLNINLADGSNFSRSAMDMNIIKHFNWVISPKLSNSGQSFHENKLKPWTEKEFG
                     IFKFIITDENDLNEVSKLIEKYNMSKDRIYIGLEGTNINTQLKPELVDKIINSGFNFS
                     PRLHVMLWGATKGK"
     gene            complement(4071..4760)
                     /gene="queC"
                     /locus_tag="PK252_13675"
     CDS             complement(4071..4760)
                     /gene="queC"
                     /locus_tag="PK252_13675"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOT15803.1"
                     /translation="MIENQKAYVLLSGGQDSFVSLLWAKKNFVEVEAVSIFYEQRHNI
                     ELQYAKAIANNFGVKHVVYDIGSFFSQLTISSLLKEGKHTESHALANQLPASFVPNRN
                     GVFLTVIANHAFKDNINPINLVTGVCQTDYSGYPDCRDNYIKAKALELSLGLDRPVNI
                     HTPLMWKTKAETFKMADEAGVLQEMIEHTLTCYNGDESLHEWGRGCTQCPACQLRKKG
                     YEEFKVMKNEK"
     gene            complement(4819..6309)
                     /locus_tag="PK252_13680"
     CDS             complement(4819..6309)
                     /locus_tag="PK252_13680"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT15804.1"
                     /translation="MSTPVFAMHGRRLFYKPEPVNRKFILNIIFLIAINLLIKPFWFF
                     GIEVAVQNRLGNELYGLYASLMSFSILFNIFLDLGVTNFNNRELARHNHLLTKYLSSI
                     IGIKLIFAAIYTVLCIVIGIIAGYETKQISILLFLVLNQVLSSLILYLRSNINALMKF
                     ITDSILSVLDKLLMIIILSILLWGINTPFKLEWFVLSQTASFLITAIVAAVIVFSNTI
                     SFKLRFDYHHMLVIIKKSLPFSLLVLLMSFYNRMEPILLERLLVNGKEQAGLYAQGFR
                     ILEVFSNFALLFTVILLPIFSKMLKEKEDTSNLVSLAFSILMIPVIVVSLTGYAYRSE
                     LMTLMYHQPGNSNILGTIIIGFIGISLTYIYGTLLTAAGKLKELNIMAASAVVINISL
                     NLILIPGFEAQGAAIASCTTQILTGIVQTIMAARILKLKKDKNILMRFAVWLFSFIII
                     LFLAKTFVSNWIFAGLVSVLLAFLFAFIFKAVHIKEIIKIAVSKES"
     gene            complement(6326..6793)
                     /locus_tag="PK252_13685"
     CDS             complement(6326..6793)
                     /locus_tag="PK252_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT15805.1"
                     /translation="MNKITINSYAEFEAYVGKELGVSEYHQVTQEQINKFAEATLDFQ
                     WIHTDPVKAAAESPFKKTIAHGYLNLSILPYLWYQIVEVNNVKMLVNYGIEKLKFGQP
                     VLVDDQVRLKASLQSISNLRGIAKAEIKVVLEIKDNPKTAMEAIVIFLYHFNN"
     gene            complement(6890..7861)
                     /locus_tag="PK252_13690"
     CDS             complement(6890..7861)
                     /locus_tag="PK252_13690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT15806.1"
                     /translation="MARNKYRFNPESMSFDRIRLSFKGLLIKLVTYFFAGIVISIVFY
                     FAFSRFFDSPKEKILKRENDQLKLQLEIFEKKLSEVSTVLKDLQQRDDNLYRTIFEAE
                     PIPRSVREAGFGGVNRYADLEGYENSDLVIETARKLDKTMKQVYVQSKSYDELIKLAM
                     NKEEMRKCVPCIQPVANKGLERTSSGFGWRIHPWYKIKIFHYGFDFAAPVGTEIYATG
                     DGVVESVENNFHGYGKKIVIDHGFGIKTLYAHLSEFKVNVGQKVKRGAVIGLVGNTGL
                     STGPHLHYEVIKNGEKVNPVNYFFNDLSPQQFEQIINISNNTGQALD"
     gene            8159..>8921
                     /locus_tag="PK252_13695"
     CDS             8159..>8921
                     /locus_tag="PK252_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOT15807.1"
                     /translation="MNSNEIREKFLEFFKTKQHQIVSSAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGNSPVTFPRIANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     EEAIAWAWEFLTEWMKLDKDRLYVTIFMGDKAENLERDTEAYDCWAKYIAKDRILDGN
                     KKDNFWEMGDTGPCGPCTEIHIDLRDDEERKKVDGKTLVNGSNPLVIEIWNNVFMQYN
                     RKASGELELLPAKHVDTGMGFERLCMAVQGKKSNYD"
BASE COUNT         2589 a         1581 c         1822 g         2929 t
ORIGIN      
        1 aataaatagg gcttcatgtg gcttgtaaag cccaacatga agccccggtt gaactaaatc
       61 ccgggcgtta atcagaaatc tatggacata tcaccttttt tttcttttca atattttcgg
      121 taccatgcaa ttttgagtca gggtacacag gtttaagacg attttctacc gtttttcgat
      181 tcaaatttgg ctattcatta aatgtattga gggcgaagaa gggttaaaac atgccatttc
      241 gtccctttag gggcatacaa accctacccc ccatgaaaat ggggggcttt tgtagcctga
      301 tattagtttt tcgaagtatt caatactgca tacaaagtat caggcgagcg tatacggggt
      361 aagatgccat aataaaatgt ttgcgctaag gtaagaaaag gatttcaatg tatatatagt
      421 ggcatagtaa ttgttacctt tgcagttcaa tgttttttgt atgttcgaga aatttaaatc
      481 ttcacaggct tttgatgttc ttcggcctgt aaatttcagg tatttcctgt tgtacaggtt
      541 ttttatgaca ctggctaccc ttatgcaatc ggtggttgtg gggtggcata tttacaaact
      601 taccggaaat gtttttgcac ttggcttaat aggccttacc gaagtgattc cgcaagtgag
      661 tgttgctttg tttgccgggc attttgttga tatctgcaac cgtcaaaaag ttatttttta
      721 tactactttt cttttgcttg caggttctgc atggttagtc atatacagta ttccggcatt
      781 cgggttgttt accaggtttg gagtaacgcc catatttatc actatatttt tgaccggatt
      841 gtcaaggggt atattaatgc ctgcccataa tgcattgctt gggcaattgg ttgagcgaaa
      901 atattttcct aaagcagcta cctggagcag tgttacatgg catatagcgg cagtatgtgg
      961 cccggccgtt ggtggtttgg tgtatggctt ttcgcaaaat gaggtggtta cttattcaac
     1021 agtttttact gcatatctga cagcttttct tttgattagc cgcataaaag tggaaaaatt
     1081 gcagacagga gaaattacag aaggcatctt tgccaggatt cacgaggggg ttaggtttgt
     1141 tttcaatagt ccatatcttt ggggagcttt tacgctggat atgtttgctg tgctttttgg
     1201 tggggcagtg gctttgcttc ctgcttttgc caaagatgtg ctgtttgttg ggccccagag
     1261 tttaggtttt ttacgggctg cgccggcaat gggggctatc ctgatgagtt tatttctgat
     1321 gttccgtccg cctgttaaaa ataccgggac gcttttattt attgcagttt cagggtttgg
     1381 gctttgtatg attggttttg ccttgtcaac ctcttttgca ttgtcactga tactgctatt
     1441 cctgagtggc atatttgaca atgtgagtgt ggttattcgt ggtacaatcc ttcaaatata
     1501 cacaccagag cacatgaaag gccgtgtttc ttctgtaaat agcattttta ttggttcttc
     1561 caatgaattg ggagcttttg agtctggctt agctgcccgc cttatggggc ttgttccatc
     1621 ggttgtattt ggcggggcta tgacacttat tgttgtcggg gtcatgtcaa aagtcaatcc
     1681 ggcgttgaaa aaaatgtctt tcgccaagca acatgtgcaa tgaaagcttt catttaatgc
     1741 ttttttgatt tgtatttttt atatttgatt caaataattg atattcatgt ttccacaata
     1801 tatacaacct gctgcttttt ctgattacga attgattgat tcgggcaatt ttgaaaagct
     1861 tgaacgcttt ggacagtatg ttcttgctcg tcctgaacct caggcaatat ggcacaaagc
     1921 tatgactgaa gatgagtgga agaacaaagc ccatgcgtgg tttaggttga acgtcaatgc
     1981 ccataacaaa gaacgcggtg agtgggtgtt gaaaaaaggt atgccccagc aatggcaggt
     2041 taattacgct tataaggaaa tgaagctgag ctttaggctt ggaatgacct cttttaagca
     2101 cgttggcttg tttcccgaac aagctgagaa ttggaatttt atttatgaaa atataaagtc
     2161 tttacaaata gcaaaccctg aggtgctcaa tttatttgcc tatacaggtg gggcttcttt
     2221 ggctgcatgc gcagcagggg caaaagtgac gcatgttgat tcggtaaagc aggtagttac
     2281 ctggtcgcgt gagaatatgg aactttctgg tttggcaaat atccgttgga tagtagaaga
     2341 tgccttgaag tttgtgcaac ggcaagtgcg taaaggtgcc aaataccagg gtattatact
     2401 tgaccctcct gcttatggcc gtggagctga tggcgaaaag tggattttgg atgaacattt
     2461 ggctgtgatg gtcgattgtt gcaacaagct tttagatgat aatggttttg tggtattgaa
     2521 tttatattct atgggatttt cgccgctggt agcacaaaac atacttgaaa catcatttgt
     2581 aggggcaaag aacgtacaat cgggtgaatt gtattttgaa gataaagcaa aacataggtt
     2641 gccgttaggg atatttgcac gtttcaataa aaattcatga cagaaaagcg aaaactcctg
     2701 cttagcttac caataccact tttttttata gtggtgatat ggtgtgtcaa gctggttgag
     2761 tatgctaaca atacttcgtt ttattttctt ggtgtttacc cacattcatt caaaggcata
     2821 attggaatac ttactgctcc tttagttcat gatgatatca aacatttggt caataattct
     2881 gtaccattcc tgttattctc ggtagcgacc ttcttttttt accggccttt gggtttaaga
     2941 gttttgttct gggtatggtt gatgtcgggt ttatgggtat ggtttggtgc tcgccctgcc
     3001 tatcatatcg gagcaagtgg tttagtttac gggttaggtg cgtttctgtt tttcagcggg
     3061 gctattcgtc gaaatgccca tttggcagcg gtttcgttaa tagtagtttt tttgtacggc
     3121 agtatgatat ggggtatctt tccttttgtt ccagacatat cttgggaagc acatctttca
     3181 ggaggtatag ctggcctgat attggcaatt gtatatagaa aagatggccc tcagcctaaa
     3241 aagtatgaat gggaagatga agagaatgtt gatagttcag atgttttaat tgataaacta
     3301 gaagagaata atagtacacc agagaaaaac taatcacttc ccttttgttg ccccccaaag
     3361 cattacatgc aggcgagggg agaagttgaa ccctgaattg ataattttat caactaattc
     3421 aggtttaagc tgggtgttaa tgtttgttcc ttcaaggcct atataaattc tgtcttttga
     3481 catgttatat ttttctataa gtttagatac ctcgtttaaa tcattctcgt ctgttatgat
     3541 aaatttaaaa ataccaaact ctttttctgt ccagggtttt aatttgtttt cgtgaaagga
     3601 ttgcccagaa ttacttagtt ttggagatat tacccagttg aaatgtttaa taatattcat
     3661 atccattgct gacctgctaa agtttgagcc atcagcaagg tttatattta ggagctccca
     3721 tggaagtata gagctattag tttctacatg aaatttcttt cctgtaactg ccaattcgtc
     3781 tagtctgtat aaggtgggct cgccaccggt aaagatgata tgatgacagg gagaatcttc
     3841 aataactttt ctgagttctt cttttgaata ttctttgaaa gtaccagatt ttctcagcca
     3901 tgtatatttt gtgtcgcacc acgagcaggt aaggttacaa aactggaaac gaataaacag
     3961 gcagttgacc cctgcatagt ttccttcgcc ctggatagac ctgaaaatct ccgatacaaa
     4021 atatttatcc attcccaaac tcagatatga ttaatattat tcgtaatttt tcatttttca
     4081 tttttcatta ccttaaattc ttcatatcct ttctttctca attggcaagc cgggcattgg
     4141 gtgcatcctc gtccccattc gtgcagggac tcgtctccgt tgtagcaggt gagggtatgt
     4201 tcaatcattt cttgcaaaac accagcttca tcagccattt taaaggtctc tgctttagtt
     4261 ttccacatca gtggggtgtg tatatttaca ggtctgtcta accccaatga caactcaagc
     4321 gctttagctt tgatgtaatt gtcccgacaa tcgggatatc ccgaatagtc agtttggcaa
     4381 acgcctgtta ccaggttgat tgggttgatg ttatctttaa aagcatgatt agctatgact
     4441 gtcagaaata cgccatttct gttgggtaca aaactggccg gaagttgatt ggccaaagcg
     4501 tggctttctg tgtgcttacc ctcctttaac aagctactta tcgtcaattg gctgaaaaaa
     4561 ctgcctatat catatacaac atgttttaca ccaaaattgt tggcaattgc cttggcatat
     4621 tgtagttcaa tgttgtggcg ctgttcatag aatatactaa cagcctctac ttcaacaaaa
     4681 ttcttctttg cccacaataa actcacaaaa gagtcttgtc ctcctgaaag cagtacataa
     4741 gctttttggt tttcaatcat attcaataag ttgaggtgca aaggtattaa aactaaaaat
     4801 caaattttga aatattcctt aagattcttt acttacggct atcttaataa tttctttgat
     4861 atgaacggct ttaaatataa aggcaaacag aaaagccaat aaaacagaaa ccaaaccggc
     4921 aaatatccaa ttggaaacaa aggttttagc taagaaaagg ataatgataa agctaaataa
     4981 ccaaacagca aaccgcatta agatattttt gtcttttttc aatttaagaa tcctggcagc
     5041 cataatggtt tgaacaattc cggtcagaat ttgtgtcgta cagcttgcta tagctgcccc
     5101 ttgggcttca aagcccggaa ttagtataag gttgagcgaa atgtttatca caacagcaga
     5161 ggcagccatg atgttaagtt ctttcagttt tccggccgct gttaatagtg ttccataaat
     5221 ataagtaagc gaaatgccaa tgaaaccaat gattatagtt ccaagtatgt tgctgttacc
     5281 cggttggtgg tacattaaag tcattaattc gctccgatat gcatatcctg taagggatac
     5341 aacaattaca ggtatcatta atattgaaaa agcaaggctc accagattgc tggtatcttc
     5401 tttttctttc agcatcttgg aaaagatggg cagaagtatg acggtaaaaa gcaaagcaaa
     5461 attggaaaaa acctcaagga tgcggaagcc ttgcgcgtat aaaccagctt gttctttgcc
     5521 atttacaagt aaccgttcaa gcaaaatggg ttccatcctg ttgtagaaag acatcagtag
     5581 taccaatagc gagaagggca aacttttctt aataatcacc agcatgtgat ggtagtcaaa
     5641 ccgcagttta aaggaaatgg tattactgaa aacaataact gcagcaacaa ttgcagttat
     5701 taagaatgat gctgtttggc tcagaacaaa ccattcgagc ttaaatggag tattgatgcc
     5761 ccatagaagt atagaaagga tgataatcat caaaagctta tccagtacag acaaaatgct
     5821 gtctgtaata aacttcatca gggcattgat gttggagcga agatataata ttaatgagga
     5881 taaaacctga ttaagcacga gaaacagcag aatagaaatc tgtttagtct catatccggc
     5941 tattatacca ataacgatgc ataatacagt atatattgca gcgaaaatca gctttatccc
     6001 tatgatagag gaaagatatt ttgttaaaag gtggttgtgc ctggcaagtt cacggttatt
     6061 gaaattagta acccctaaat caaggaaaat attaaaaagt atcgagaaac tcattagtga
     6121 agcataaagc ccgtaaagct cgtttcctaa cctgttttgg acagcaactt caatgccaaa
     6181 aaaccaaaaa ggtttgatga gcaaattgat agctataaga aatatgatat ttaaaatgaa
     6241 cttacggttc acaggttcag gtttataaaa taaacgcctg ccatgcattg caaagacagg
     6301 cgttgacatt tagttataat agtagttaat tgttgaaatg gtataaaaat attacaatag
     6361 cctccatggc tgttttaggg ttgtctttta tttctaaaac tacctttatt tctgctttgg
     6421 ctatgccgcg taaattgctt atgctttgta aactggcttt taaccttacc tggtcgtcaa
     6481 ccaacactgg ttgtccgaat ttaagctttt caataccata gtttaccagc attttcacat
     6541 tgtttacttc aactatctgg taccaaaggt atggcaatat agataagttg aggtatccgt
     6601 gtgcaatggt ctttttaaaa gggctttctg cagctgcttt taccgggtcg gtgtgtatcc
     6661 attgaaagtc gagtgtcgcc tcggcaaact tgtttatttg ttcttgtgtt acctggtgat
     6721 attctgatac tccaagctct ttgccaacgt aggcttcaaa ttcagcatag ctgttgatgg
     6781 ttattttatt catttttgtt gtttttagtt gggcaaatgt aaaaaaaact tcaaacatac
     6841 atctttaaaa gcatgtttga agtttttgct tcaaaaagaa tgtaaaaaat tagtcgagcg
     6901 cctggcctgt attgtttgag atattgataa tttgctcaaa ctgttgaggt gataagtcat
     6961 taaagaagta gttgaccggg tttacttttt ctccattttt aataacttca taatgcaagt
     7021 gtggcccggt tgatagaccg gtattgccta ccaatccaat aacagcccct ctttttactt
     7081 tttgtcctac attaacttta aattcactca ggtgggcata aagtgttttt attccaaagc
     7141 cgtggtcaat aacaattttt ttgccatagc cgtgaaaatt attctcaaca gattcaacaa
     7201 ctccatcacc tgttgcatat atttcagtcc caacaggggc tgcaaagtca aacccgtaat
     7261 ggaaaatctt gattttatac cagggatgga tacgccagcc aaagcctgaa gatgtccttt
     7321 ccaagccttt atttgctact ggctggatac aaggtacaca tttacgcatc tcttctttgt
     7381 tcatggcaag tttgataagt tcatcatacg acttagactg aacataaact tgtttcatgg
     7441 ttttatctaa tttcctcgcc gtttcaataa ccaaatctga attttcatac ccttcaaggt
     7501 ctgcatagcg gtttacaccc ccaaaaccag cttccctgac cgaacgtgga ataggctctg
     7561 cttcaaatat ggtacggtaa agattgtcgt cacgttgttg taagtcttta agtactgtac
     7621 ttacctcact taactttttt tcaaatattt ctaactgtag tttaagctga tcattttcac
     7681 gttttaaaat tttttctttg ggagagtcaa aaaagcgtga aaaagcaaaa taaaatacaa
     7741 tggaaattac tattcctgca aagaaatacg tgaccagctt aataagcaac cctttaaaac
     7801 tgagcctgat acgatcaaaa ctcatagatt cggggttgaa gcgatattta tttcttgcca
     7861 taataatctg ttttttaatt ggttagttta agttccccaa aattgcgaca aaactaaggg
     7921 ttttatctaa agaaaacaaa ttttttagat aaaactttga ttttcagcct atattaaatt
     7981 acaattacgc cccaaaacgt ttattatact tatgaaaagc aaaaatgttg gatacttatg
     8041 tctttttttt acataaaaaa tgcataaatc tgtttaacaa tttgattatt ccattttatc
     8101 acattgtaaa tatgttaatt ttgtgcgtca aattttgtaa gttaaaatac ataagtatat
     8161 gaattcaaac gagatacgtg aaaaattttt agaatttttt aaaactaaac agcaccagat
     8221 agtttcttca gcccctatgg ttgttaaaaa tgacccgact ttgatgttta ccaatgccgg
     8281 gatgaaccag tttaaagata tttttctggg taattcacct gtaacttttc cccgaattgc
     8341 caatagccaa aaatgtttga gggtttcggg caaacacaac gacctggaag aagttggcca
     8401 cgatacctac caccatacca tgtttgaaat gcttggtaac tggtcgtttg gtgattattt
     8461 taaagaagaa gccattgctt gggcttggga attccttacc gaatggatga aactcgataa
     8521 agatcggtta tatgttacca tatttatggg tgataaagcc gagaaccttg aaagggacac
     8581 agaagcttat gattgttggg caaaatatat tgccaaagat cgtatccttg atggaaataa
     8641 aaaagacaac ttttgggaaa tgggcgatac agggccctgt ggcccttgta ccgagattca
     8701 cattgacttg cgtgacgacg aagaaagaaa aaaagtggac gggaaaaccc ttgttaatgg
     8761 tagcaaccca ttggtgattg aaatttggaa caatgtgttt atgcaataca accgcaaagc
     8821 ttctggcgaa ttagagcttt taccagctaa acatgttgat acaggtatgg gctttgagcg
     8881 tttatgtatg gctgtgcagg gcaaaaaatc aaattacgac a
//
LOCUS       DAOSPF010000104         8750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8004_length_8750_cov_7.546866, whole genome shotgun sequence.
ACCESSION   DAOSPF010000104 DAOSPF010000000
VERSION     DAOSPF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8004_length_8750_cov_7.546866"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1199)
                     /locus_tag="PK252_13700"
     CDS             complement(66..1199)
                     /locus_tag="PK252_13700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT15808.1"
                     /translation="MNPTIDKMSPFIVMEVLEKAQEMEKRGINIIHMEVGEPDFSVPV
                     CVSEASIKAVAEGYTHYTHSLGMPELRDELARFYKKEYNVTVDPECIILTSGSSPAIL
                     TLLMLLCNTGDEVLVSNPGYACYRNFALACHAIPVDVPLSAHNGFQFDINEVLKAKTD
                     KTRAMFINSPMNPCGVLIKDEVFEGLVKTGIPIISDEIYHGLVYEGKTRSILEFTNEA
                     FVVSGFSKRFAMTGLRLGYLIAPKKYIRSLQILQQNLFICAPSVSQYAAIAALKSAEE
                     DVNSMRQVYNERRVYMISRLRAMGFIIETEPQGAFYIFADAGKFTNNAYQFAFEVLEN
                     AHVGITPGTDFGSRGEGFVRFSYANSLENLKEGLDRLERYLKN"
     gene            complement(1526..3286)
                     /locus_tag="PK252_13705"
     CDS             complement(1526..3286)
                     /locus_tag="PK252_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT15809.1"
                     /translation="MNYNLNKQKEEGKNASTFSSLKMFLALIKEEKKTLWFAFFFIIL
                     NAFLSLFGPYFIAHTIDTYVAAKNYHGIMLNALILLAIYIVAFGANYLQMILMGGVGQ
                     RMLFSLRNAVFNKLQSLPVDFFHQNKAGDLISRINNDTDKLNIFFSQSLMQFIGNIIT
                     MLGSAIFLLTLNFPLGLAALVPAVLIWIFTKSTSPWVKRRNALNLKSVGALSGEVQES
                     LDNYKVIVAFNRRDYFRKKFEEANEDNYKTSVDAGISNNIYLPVYSFAANIGQLIVLG
                     LGIYLITKGAFTIGLLIGFIAYVNNFYFPLRHLAGLWANFQVAMAGWDRIRKILNLEN
                     NLTVVENKVQEENPAYIEFRDVSFTYPNGKEVLHHINIKLERGKTYAFVGPTGGGKTT
                     MASLIARLYDTSKGHVLLEGRDIRSFEADERTRKIGFILQEPFLFSGTVRENILYGNE
                     NLQAYTNGQLAKLIHESGLETLLGRFEQGLDTQVFTTGDSISLGQKQLIAFMRAVLRK
                     PELIILDEATANIDTITEKLLSDILLKLPASTTRVVIAHRLNTIENADEIFFVNSGKV
                     TPAGSLEDAVNMLLHGKRAS"
     gene            complement(3276..5024)
                     /locus_tag="PK252_13710"
     CDS             complement(3276..5024)
                     /locus_tag="PK252_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT15810.1"
                     /translation="MKKKDTARNGVSKGSPNMFSILKPYKIWIIGLVIAALVGNGVNL
                     VLPKIIASTIDSYNAGNYSLKTLVISFLVASSLIFIFTYLQSILQTYASEKVAKDLRA
                     KISDKISQQSYSYVLKANPSKLLTNLTSDVDSIKMFVSQAVATIISSFFVIIAISVII
                     IHLNWKLALPILAIIPIIGGTFFFVLGKVKVQFKRSRENIDRLNRTINESIMGSSLIR
                     VINSQSIEFEKFLTVNTSARDIGMAILRMFAWLIPIIVFVSNIAVIVILALGGHFVVQ
                     GSMSVGDFAAFYSYLALLIFPILMIGFMSNIIAQASASYRRISEVLNAPVIVAEGKDE
                     STLTGKIEIQEVSLNYGDKPVLKNVSFVVGAKSHTAIIGPTAAGKSQLLYLLSNIIKP
                     ASGKILFDGKSIDTYDCNTFYKQIGIVFQDSVIFNLSLRENIAFSGEASEEAIQKAIE
                     TAELKEFVDTLPAKLDTVVSERGSNLSGGQKQRIMLARALVLEPKILLLDDFTARIDR
                     KTENKILCNIENNYPHITIVSVTQKIASIKHFEKIVLLMEGEIIAEGTHKELLETCPE
                     YVQVYNSQRSTSHYEL"
     gene            complement(5139..7151)
                     /gene="uvrB"
                     /locus_tag="PK252_13715"
     CDS             complement(5139..7151)
                     /gene="uvrB"
                     /locus_tag="PK252_13715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT15811.1"
                     /translation="MDFKIVSDYTPTGDQPEAISRLVQGINNGDKFQVLLGVTGSGKT
                     FTIANVIEKVQRPTLILSHNKTLAAQLYGEFKSFFPNNLVEYFVSYYDYFQPEAYLPV
                     TDTYIEKDLSINDELEKLRLSATSALLSGRKDVIVVSSVSCLYGIGNPEDFHSNIVKI
                     TVGQNVGRNQFLRKLVESLYSRNEVEFKRGTFRVRGDTVDISLPYSDMAYRITFWGDE
                     VESIESFDPINGLSIEPMDHAMIYPANIFVTTQERIHGAIKEIQEDMMMQVQYFKNQG
                     KNLEAKRIEDRVTYDIEMIKELGYCPGIENYSRYFDGRKAGTRPFCLIDYFPEDFLMV
                     IDESHVTIPQIRAMYGGDNSRKKTLVEYGFRLPAAIDNRPLKFEEFEALTPQTIYVSA
                     TPAEYELEKSGGVIVDQVIRPTGVLDPIIEVRPSLNQIDNLIGEINTCATKDERVLVT
                     TLTKRMAEELTDYLTKKGIRCQYIHSDVDTLERVKIMENLRNGHYDVLIGVNLLREGL
                     DLPEVSLVAIIDADKEGFLRSERSLTQTAGRAARNINGRVIMYADKITESMQRTIDET
                     NRRREKQLKYNEQNGLVPRQVVRAVNSIMTTLDKNRTVKAYMDVDETDVAADPLVQYM
                     DKKALQKAIDKAKKSMEAAAKELDFLEAARYRDEMYALQKILDTKK"
     gene            complement(7212..7574)
                     /locus_tag="PK252_13720"
     CDS             complement(7212..7574)
                     /locus_tag="PK252_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15812.1"
                     /translation="MTDKKKVLFIDDEPINLQLFAINFSRKYHVLTALSGSQAIEVLT
                     HEPEVVAAVCDMKMPVMDGIEFVTIAKNRFPLVKYIILTGSGLTPEISRAIEDGRIYK
                     YFCKPFGTTEIETCLENL"
     gene            complement(7571..>8750)
                     /locus_tag="PK252_13725"
     CDS             complement(7571..>8750)
                     /locus_tag="PK252_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15813.1"
                     /translation="ASPKGEGFHPSQNYKLKMPKKDILGKAYDRLNINFDKEKLKYIN
                     DELQEKGFVENLEIESITSDGDTVYTYYSCRLIRMRGEPVVIHSSVNITEKKKIEQEL
                     EDYRKNLEKLVKERTEALEIANKALEDTNKKLQKTNEELNRQRLELQQALQNLNKTQV
                     QLVESEKMASLGVLAAGVAHEINNPLNFIHGGSMGIENYMNDYLVDHKEQLEPMINAI
                     NTGVHRAARIVASLSHFSRSTDSIEEQCNIHAIIENCLVMLQNQIKNKATVIKEFTDK
                     PFVMTGNDGKLHQVFLNIIANAVQAIPEKGEIKISTQIKENYLKIAITDNGLGIEKKH
                     LSRIFDPFFTTKPPGKGTGLGLSIVNKILIEHKGHISVDSELGKGTTMNITLPIIKTL
                     "
BASE COUNT         2617 a         1840 c         1644 g         2649 t
ORIGIN      
        1 gttgagaatc ctaagtatca ttgacttgaa aattcaaaga gaatatacct taagtaaata
       61 atttgctaat ttttcaagta cctttccagc ctgtccaatc cttctttcag gttttccaat
      121 gaattggcat acgagaacct tacaaaacct tcgccacggc taccaaaatc cgttccggga
      181 gtaatgccaa catgggcatt ctctaatact tcaaatgcaa actgataggc gttattggta
      241 aatttgccgg catcggcaaa gatataaaat gcaccctgcg gctcagtctc aataataaag
      301 cccatagccc tgagacgaga aatcatgtaa acacggcgct cattgtacac ctgtctcatc
      361 gagtttacat cttcctcagc gctttttaat gctgcaatag ctgcatattg cgacaccgaa
      421 ggagcacaga taaaaaggtt ttgctgcaaa atttgtaaag aacgaatgta tttctttgga
      481 gcaataagat atcctaaccg aagcccggtc atagcaaaac gtttggagaa accacttacc
      541 acaaaagctt cattggtaaa ctccagaatg ctccttgtct tgccctcata caccaaacca
      601 tgataaatct catccgagat aattggaata ccagttttta caagcccttc aaaaacctca
      661 tccttgataa gtaccccgca aggattcatc ggagaattga taaacattgc acgagtcttg
      721 tccgtcttag ctttcagcac ttcatttata tcaaactgaa atccattatg ggcactcagc
      781 ggaacatcaa ccggaatggc atgacaggca agggcaaaat tgcgataaca agcatatccc
      841 ggattagaaa caagcacttc atcacctgtg ttgcacaaaa gcatcagcag cgtaagtatt
      901 gcaggagatg aacctgatgt aagtattata cattcagggt caacagtaac attgtattct
      961 tttttgtaaa aacgagccaa ctcatctcga agctcgggca tcccaagcga atgagtataa
     1021 tgcgtatagc cttcagcaac agcctttatt gaagcttctg aaacgcaaac aggaacagag
     1081 aaatcgggtt caccaacttc catgtgtata atgttgatgc cgcgtttttc catttcctgc
     1141 gccttttcga gcacttccat gacgataaac gggctcatct tatcaatagt aggattcatt
     1201 gtaacaaagt gatggtaaaa cgaaagtgca aatatcgggt attagaaata aaatgaacac
     1261 gtttttattg agaaatgctt gatataagag cttacaagct aaatgcaact tttttatcga
     1321 aaaaagttgc tcaaaaactc ttatcgcctt aactctttca ccctcgaagc cttgtaatgt
     1381 tgcaatatgc aaaaacagga gtgttaggct tctcggccga aatcgaacaa aaaggcgatg
     1441 aaaaggaatt atttttaatg ctccgcagac tttgttttaa agtcacttca gaacaatacg
     1501 cagaatcttc taaaaacaga ataactcagc ttgcacgttt tccgtgtagc agcatattta
     1561 ccgcatcttc caaagagcca gctggggtta cctttccaga gttgacaaaa aagatttcat
     1621 cggcattttc aatggtatta agcctgtgag caataacaac gcgtgttgta gaagccggga
     1681 gtttcaacag tatgtcagat agcagcttct cggtaatggt atcaatattg gccgtggctt
     1741 catccaggat tatcagttca ggcttacgaa gcactgcacg cataaaggct atcaactgtt
     1801 tttgtccaag actgatggaa tctccagtgg taaacacctg agtatctaaa ccttgctcga
     1861 aacgtcctaa aagggtttct aagcctgact catgaataag cttagctaat tgaccgttgg
     1921 tatatgcctg taagttttca tttccgtata agatattttc ccgaacagtt ccggaaaaga
     1981 gaaatggctc ttgcaaaata aaaccaatct ttcgcgtccg ctcatctgct tcgaacgagc
     2041 gaatgtcgcg cccttcaaga agcacatgac ctttgctggt atcgtacaaa cgagctatca
     2101 acgaagccat agttgttttt ccaccaccag tagggcctac aaaagcatag gtctttcccc
     2161 tttctaactt aatattgata tggtgcaaca cctctttgcc attgggatag gtaaacgaaa
     2221 catcgcgaaa ctcaatatat gcaggatttt cttcctgaac tttattttca acaacggtaa
     2281 ggttgttttc cagatttagg attttacgaa ttctgtccca gcctgccata gcaacctgaa
     2341 agtttgccca aagtcctgcc agatggcgga gcggaaaata aaaattattg acataagcaa
     2401 taaagccgat tagcaaacca atggtaaacg ctcccttagt aataaggtat attcccagtc
     2461 caagaacgat caactgaccg atattggcgg caaagctata taccggcagg taaatattat
     2521 tggaaatacc agcatctacg gaagttttat aattatcctc gttagcttcc tcaaatttct
     2581 tgcgaaaata atctctacgg ttgaatgcta ctataacctt ataattgtcg aggctttcct
     2641 gcacttcacc actaagagcc ccaacacttt taaggttcaa agcattgcgg cgtttcaccc
     2701 aaggcgatgt ggactttgta aaaatccaaa ttaatacagc tggcaccaat gcagctaatc
     2761 caagcggaaa attcagcgta agcaaaaata ttgcagagcc cagcatagta attatgttac
     2821 caataaattg catcaacgat tgcgaaaaga atatattgag cttatcagta tcattgttga
     2881 tacgcgaaat caaatcgccg gctttgttct ggtgaaagaa atcaacaggc aggctttgca
     2941 acttattgaa cacggcattt cgtaggctaa acagcatacg ctggcctact ccacccatca
     3001 aaatcatttg taagtaatta gccccaaaag caacaatgta gattgccagc aaaataagag
     3061 catttagcat gataccatga taatttttgg ccgccacata agtgtcaatc gtatgggcaa
     3121 tgaaataagg gccaaaaaga cttaaaaaag cgtttaggat aatgaagaaa aaggcaaacc
     3181 aaagcgtttt tttctcttct ttgataagcg ctaaaaacat tttcagcgag gaaaaagtag
     3241 aagcattctt tccttcttcc ttttgtttat tgagattata gttcataatg gcttgtactt
     3301 ctttgtgaat tatagacctg aacgtactca ggacaagttt cgagcaattc tttgtgggta
     3361 ccttctgcaa taatttcgcc ttccatcagc aaaactatct tttcaaaatg cttgatagaa
     3421 gctattttct gggttacaga aacaatggta atgtgagggt aattattttc gatattgcac
     3481 agaatcttgt tttccgtttt tcggtcaatg cgggcagtaa agtcgtccag caagaggatt
     3541 ttcggctcaa gtactaatgc cctggctagc atgatacgct gtttctggcc tccagaaagg
     3601 tttgagcccc gctcagaaac tacagtatcc aattttgcag gcaaagtatc gacaaactct
     3661 ttcaactcag ccgtttctat agccttctga atggcttctt ctgacgcttc accactaaag
     3721 gcaatatttt cgcgcaagct aaggttgaaa atcacgctat cttgaaaaac tatgccaatt
     3781 tgtttataaa atgtattaca atcgtatgtg tctattgatt ttccgtcaaa aagaattttt
     3841 cccgaagcag gtttaataat atttgaaaga aggtaaagca actgactttt gccagcagca
     3901 gtaggcccaa tgatggcagt atgacttttt gcaccaacca caaagcttac attctttagc
     3961 actggcttat caccatagtt caacgacact tcctgtattt cgatttttcc cgtaagggta
     4021 ctttcatctt ttccttctgc aacaattact ggagcattta acacctctga aattcttctg
     4081 tacgaagccg aagcctgggc tataatattg ctcataaagc caatcatgag tattggaaag
     4141 atgagcaagg ccagataact ataaaatgct gcaaaatcgc caacagacat agaaccctgc
     4201 accacaaaat gcccgccaag ggcaagaata acaataaccg ctatgtttga aacaaagaca
     4261 atgattggaa ttaaccaggc aaacatacgt aaaatagcca tgccaatatc acgtgcagaa
     4321 gtattgacgg tcaggaactt ttcaaactca atactttgcg agttgataac cctgataagt
     4381 gaagagccca tgatactttc gttaatagtt ctattcaaac ggtcaatatt ctcacggctt
     4441 ctcttaaact gcaccttcac ttttcccagc acaaaaaaga atgttcctcc aataatcgga
     4501 ataatagcaa gtataggtaa tgccagcttc caattgagat ggataataat cacgctgata
     4561 gcaataatga caaaaaacga tgaaattata gttgcaacgg cctgcgaaac aaacatcttt
     4621 atcgaatcaa catctgaagt aagattggta agcaatttag aaggattggc tttgagaaca
     4681 taagaataac tttgctgcga aatcttgtcg gatatttttg cacgcaggtc tttagccact
     4741 ttttctgaag cgtaggtttg taaaatactt tgcaggtaag taaaaataaa aatgagggat
     4801 gacgcaacta aaaaacttat tacaagcgtt ttcagactgt agttcccagc attatatgaa
     4861 tctatggtag atgcaataat ttttggcagt acaaggttga ctccatttcc aacgagtgcg
     4921 gctattacca aacctataat ccaaatcttg taaggcttta atattgaaaa catgttgggg
     4981 ctcccttttg aaacgccatt tcttgcagtg tcttttttct tcatcttact tttctcaact
     5041 ttcaacaaaa caaattaatc ccggttttgt ggatttcgta aacaaaattt cggcaagatg
     5101 ccattttgcg gcggtcaata agcttttatg gattatggtt attttttagt atccaaaatc
     5161 ttttgtaaag cgtacatttc atcgcgataa cgggcagctt caagaaaatc aagctctttg
     5221 gcggcagctt ccattgactt cttagcttta tcaatagctt tctgcaaggc ctttttgtcc
     5281 atatactgaa ccaatgggtc agcagcaacg tctgtttcat caacatccat atacgccttg
     5341 accgtcctgt tcttgtccaa agtggtcata atagaattaa ctgctctcac cacctgacga
     5401 ggaacaagcc cattctgttc gttatatttc aactgctttt cgcgacgacg gtttgtttcg
     5461 tcaatagtgc gctgcatact ttcggttatc ttatcagcat acatgataac cctgccgttg
     5521 atgttacggg cagcacgtcc tgcagtttgc gtcaatgaac gttctgagcg gagaaagcct
     5581 tccttatccg catcaataat ggcaaccaac gaaacttctg gcaggtcgag cccttcacgc
     5641 aaaaggttta cacctataag tacatcgtaa tgcccgttgc gcaagttttc cataatttta
     5701 acacgctcaa gcgtatctac atctgagtgt atatattggc aacggattcc tttttttgtc
     5761 agataatcag taagctcttc ggccatgcgt tttgtgagtg tagtaaccag tacacgttca
     5821 tcttttgtag cacaagtgtt aatctcgcct atcaagttat caatctggtt aaggcttgga
     5881 cgcacttcaa taattggatc aagcactccc gtagggcgta tcacctggtc aactatcaca
     5941 cctcctgatt tttcgagttc atattcagca ggagtggcgc taacatatat ggtttgaggg
     6001 gttagcgctt caaactcttc aaatttcagc ggcctgttgt caatagcagc aggtaaccta
     6061 aagccatact caaccaacgt cttctttcga gaattatctc cgccatacat tgccctgatt
     6121 tggggtattg taacatggct ttcatcaata accatcagaa aatcttcagg gaaataatca
     6181 atcaaacaga atggcctggt cccagcctta cggccatcga aataacgaga gtaattctcg
     6241 atacccgggc agtatccaag ttctttaatc atctctatgt catacgtcac acggtcttca
     6301 atacgtttag cctccagatt cttgccctgg ttcttgaaat attggacctg catcatcatg
     6361 tcttcctgaa tttccttgat agcaccgtgt atcctttctt gtgtagtcac aaaaatattg
     6421 gccggatata tcatggcatg atccattggc tctatagaga ggccgtttat tgggtcaaac
     6481 gactcaatac tttcaacttc atcgccccaa aatgtgatgc gataagccat atcgctatat
     6541 ggaagggata tgtcaacagt gtctcctctg accctgaatg tgccccgctt gaattccacc
     6601 tcattgcgcg aatataagct ttctacaagc tttcgcagaa actggtttcg tccaacgttt
     6661 tgaccaacgg tgatttttac aatattacta tgaaaatcct ccggatttcc aatgccataa
     6721 aggcatgaaa cagacgatac cacgatcaca tctttccttc cagaaagtag tgctgaagta
     6781 gcactcaagc gtagtttttc caactcatca ttaatagaaa ggtctttctc aatataagta
     6841 tctgtaactg gcagatatgc ctcaggttgg aaatagtcgt aatacgaaac gaagtattca
     6901 acaaggttat ttgggaaaaa agatttgaac tccccgtaaa gctgggcggc cagggtttta
     6961 ttatggctta gtatcagcgt ggggcgttgt accttttcaa ttacattggc tattgtaaag
     7021 gttttcccgg aacctgtaac cccaagtaaa acttgaaatt tgtcaccatt atttatacct
     7081 tgaactaagc gggaaattgc ttcaggctga tcacctgtgg gagtataatc gctgacaatc
     7141 ttgaaatcca taggatacaa attcattacc cgacaaaggt agtgaatata cgtttgcctt
     7201 atgaagaaaa actataagtt ttcgaggcag gtttcaatct cagtagtgcc aaatggtttg
     7261 caaaaatact tataaatacg tccatcttcg atggcccgag aaatttcagg agtgaggcca
     7321 gaacctgtta ggataatata ttttaccagc gggaaacggt ttttggcaat ggtgacaaat
     7381 tctatcccgt ccatcacggg cattttcata tcgcacactg cagcaactac ttcaggctcg
     7441 tgggttaata cttcaatggc ctgagagcct gaaagtgcag tgagtacgtg atacttccgg
     7501 ctgaaattta tagcaaatag ctgaaggttt ataggttcgt catcgataaa taatactttt
     7561 ttcttgtctg tcataaggtt ttgataattg ggagtgttat attcattgtg gttccttttc
     7621 ctaattcaga atcaacagag atatgacctt tatgttctat aaggatttta ttgacaatag
     7681 ataagcccaa gccagtccct ttccctgggg gtttggtggt aaagaatggg tcgaaaattc
     7741 ttgataaatg ttttttttca attcctaaac cattgtctgt aattgctatt ttaagatagt
     7801 tttcttttat ctgggtcgaa atttttattt cacctttttc aggaatagcc tgaactgcat
     7861 tggcaatgat gtttagaaat acctggtgca actttccgtc attgcctgtc attacaaacg
     7921 gtttatcagt aaattcctta ataacagtag ctttattctt aatttggttt tgcagcatca
     7981 caagacagtt ttcaataata gcatgaatat tacattgctc ttcaatagag tcggtactgc
     8041 ggctaaaatg gctcaggctg gccacaattc gggcagcacg gtgtacgccg gtattgatgg
     8101 catttatcat tggttcaagc tgttctttat ggtcaacaag gtaatcattc atgtaatttt
     8161 caatacccat agagcctcca tgtataaagt taagaggatt gtttatttca tgggctacac
     8221 cagcagccag cacccccaaa gatgccattt tttcggactc aactaattgt acttgtgttt
     8281 tgttcaggtt ttgcaaggct tgttgaagtt ctaatctttg tctgttcaat tcttcattgg
     8341 ttttttgtaa ttttttattg gtatcttcta aggctttatt agcaatttca agagcttcgg
     8401 tgcgttcctt gacaagtttc tcaagatttt tacgataatc ttccagttct tgttctattt
     8461 ttttcttttc ggtgatattt accgaagaat gaattaccac aggttcgccc ctcatcctga
     8521 taagtctgca ggaataataa gtatatacag tatctccgtc agatgtaata ctttctattt
     8581 cgaggttttc aacaaatccc ttttcctgta attcatcatt tatatatttt agcttctctt
     8641 tgtcaaaatt tatgttaagc ctatcataag ccttgccaag aatgtctttt ttaggcattt
     8701 ttaatttata attctgggat gggtgaaatc cctcgccttt aggcgaagca
//
LOCUS       DAOSPF010000105         8419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8388_length_8419_cov_6.262912, whole genome shotgun sequence.
ACCESSION   DAOSPF010000105 DAOSPF010000000
VERSION     DAOSPF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8388_length_8419_cov_6.262912"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1339)
                     /locus_tag="PK252_13730"
     CDS             complement(299..1339)
                     /locus_tag="PK252_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HOT15814.1"
                     /translation="MIQIDDFAIQSIAVHYVGNKLNDEVCAISKGLLNTNEKTLYALV
                     KYFRSSFKSQEYYNFFHESDINLNEAYTFAGKIFENPDVLYEQSVNLAQHLYAQCVHP
                     KIKSGEFYVVYFKDCMLDDYVADAIGLFKSENKDTFLQVSATDDGLNIESETGININK
                     PDKGCLIFNIEKDKGYVVAVVDNTNKGLEAQYWIDSFLKIRQRNDEYLKTQQMMSLCK
                     DFITKELPQQYNTTKADQVDMLKKSVKFFKEKEHFEMDEFTNEVIGQPDVIETFNNYK
                     NNYQTEQELHFDNNFNINNTAVKKQERKFRSVIKLDKNFHIYVHGNPNLIEQGIDENG
                     RKFYKIFYQEEN"
     gene            1463..2014
                     /locus_tag="PK252_13735"
                     /pseudo
     CDS             1463..2014
                     /locus_tag="PK252_13735"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
     gene            2018..2524
                     /locus_tag="PK252_13740"
     CDS             2018..2524
                     /locus_tag="PK252_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT15815.1"
                     /translation="MKLKKEEWLPVVNEKGEIIGKAPRSACHSDKSLLHPVVHLHVIN
                     NRGEIYLQKRPMHKIQPGKWDTAVGGHISFGESIETALVREAEEEIGIIGFKPKLVAN
                     YIWESDIERELVFCFVTHYQGAININKEELTDGRFWTHNELKDAIGKEILTPNFEIEY
                     QKVLSKFK"
     gene            2609..4111
                     /locus_tag="PK252_13745"
     CDS             2609..4111
                     /locus_tag="PK252_13745"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT15816.1"
                     /translation="MAQIINDISRTFNEYLLIPGLTTKDCTPDRITLKTPLVKFAAGS
                     KAEISLNIPFVSAIMQSVSDSNMAIALARNGGLSFIYASQTIEDQAEMVRKVKKFKAG
                     FVVSDANLTPENTLKDVIELNERTGFSTIAITHDGTAGGKLLGMVTSRDYRPNKDSLD
                     KKVKDFMTPFSKLVVGKYGISLDEANDIIWENKINTLPIIDENQCMKYFVFRKDYDNH
                     KDNPNELLDSHKRLLVGAGVNTRDYKERIPRLVEAGVDVLCIDSSDGFSEWQKDTIKF
                     VRDTYGNTVKIGAGNIVDEDGFNYLADAGADFIKVGIGGGSICITREQKGIGRGQATS
                     LIEVAKARDEYFKKKGEYIPICSDGGIVQDYHMVLALAMGADFIMMGRYFARFDESPT
                     KKLLIGSNYVKEYWGEGSNRARNWQRYDMGGNSSLKFEEGVDSYVPYAGKLKDNLDVT
                     LGKIKSTMCSCGALSIPELKKVAKITMVSSTSIIEGGAHDVILKETKRED"
     gene            4333..4950
                     /gene="nth"
                     /locus_tag="PK252_13750"
     CDS             4333..4950
                     /gene="nth"
                     /locus_tag="PK252_13750"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT15817.1"
                     /translation="MQPWSEKIEPLLKLYGKRKHPLDYQNRYQLLVMVILSARDSDKH
                     INELSKSFFEQYPTMSHLAKASIVELHQCLGTITNFANKAEWLLKIAQTIGEDNKIPA
                     TMAELTKLPGIGRKSANVIIRESGFEAEGVIVDLHVVRVAPRLGIATGSNPEKIEKQI
                     MSAIPQARWNETGMAISFLGREICRPSNPKCEQCVMNSCCEHNGV"
     gene            4980..6566
                     /locus_tag="PK252_13755"
     CDS             4980..6566
                     /locus_tag="PK252_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT15818.1"
                     /translation="MKHLLIITAACILASPIFAQSKKNKQALPERVINIDFSAEKGPL
                     NTMFKKCVGAGRANEGLRADWQQQLAYVKKECDFKYIRMHGLLTDDMGVYKEDKNGNP
                     QYNYMYVDVLMDYLLSIGMKPFVELGFMPNALASGEKTIFWWKGNVTPPKDYDKWGAL
                     IKNLTEHFTERYGADEVKTWYFEVWNEPNLVPWFFTGTQEDYFKLYEYSAKAIKSVNK
                     DYRVGGPGTAGAAWETELIEFCAKNNLPIDFVSTHSYGVKQGYLDEYGNSGTILDKNP
                     MSVSGDVIQSRKEIANSSKPNLELHYTEWSASYTPSDPIHDSYHEASYILQKLKQVGT
                     AAQSMSYWVFTDIFEESAPRFEPFHGGFGLLNIQGINKPAFYAYQFLNRLGNIELTNT
                     DTASWVCKDANGNIQVLAWDFTNTHPGDSVNNQVYYIRDLPSKSKGKLKISISKVPAG
                     IYSLEVYKVGYRCNDAYTSYYDLGRPKKLTRLQVEQIKKQNDGSPVEKEIVKVKEAIS
                     FVKELDIRENDVYLLKLTKL"
     gene            complement(6620..7225)
                     /locus_tag="PK252_13760"
     CDS             complement(6620..7225)
                     /locus_tag="PK252_13760"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOT15819.1"
                     /translation="MNWLNIITYSGTFVFAITGTLKARTHKMDVFGAAVLAFCTAYGG
                     GTIRDILLGIRPVNWLGDYFALTLVISAVLIIFLFKTNMDKFNRVIFITDAIGLGMFT
                     IAGIQISMQAGIGSVYSVIMGVITATFGGLVTDILCNNIPDLLKNGEMYATACFIGGL
                     VFILLIKLGIHPDVCILICVLLIVSLRVLSKVKKIHIPRFK"
     gene            7488..>8419
                     /locus_tag="PK252_13765"
     CDS             7488..>8419
                     /locus_tag="PK252_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT15820.1"
                     /translation="MFGLDNGMAFILIICIIAACFFEFINGFHDTANAVATVIYTNSM
                     KPGVAVVWSGVFNFIGVYMGGIGVAIGIINLLPMSLLTDQSVNHNIAMILSIIISSII
                     WNLGTWYFGIPCSSSHTLLGSLFGVGLAFSLLPGVENVGLNWTKVKDAGLSLMLSPLI
                     GFGLTMGIVILVKKISKKLKNNILFEEPQKKKSPPPLVRGLLVLTCTSVSYSHGSNDG
                     QKGVGLLMIILIALAPLSFSVNHSMQPEKLLVSLNQIDSIVSKIDYTGLTDIEKGTLT
                     EANKRLDSMQVILADINSFDSLKNGQPQALRKNIL"
BASE COUNT         2702 a         1421 c         1687 g         2609 t
ORIGIN      
        1 aaacaaaagc aattaaaaat aaagagaagc aaaatttcaa attcataatt attattttat
       61 ttcttaaatt ctttaacggt tattcttgat aatttgtttc tatttttatg acaaaagtca
      121 tacttttttt ggatattttt taatataatc atataaaaat ttaatatcta acactttatg
      181 tttgtcttat acaataaaat gttgatgcaa taaaaagctt taaattatca ataggtaagg
      241 ttattgaata aataacggat aaacattgtt agtttagaaa agtaataata atcatcagtt
      301 agttttcttc ctgataaaat attttataaa atttccttcc gttttcgtca atgccttgtt
      361 caataaggtt gggattgcca tgcacgtaga tgtggaagtt tttatcgagt tttattacgc
      421 ttctaaactt tcgttcctgt tttttaacgg cggtattatt gatgttaaaa ttgttgtcaa
      481 aatgtagctc ctgctctgtc tggtaattgt ttttgtaatt attgaaggtt tctataacat
      541 caggctgtcc aattacttcg ttggtaaatt catccatctc aaagtgctct ttttctttaa
      601 aaaatttgac agattttttc agcatgtcca cctggtcggc tttagtggta ttgtattgct
      661 gtggaagctc tttggtgata aaatctttgc agaggctcat catttgttga gttttgagat
      721 attcgtcatt gcgttgcctg attttcagga agctgtctat ccagtattgg gcctcaagcc
      781 ctttgttggt attatcgaca acggcaacca cgtaaccttt gtccttttct atgttgaaaa
      841 tcaggcaacc tttgtcaggt ttgttgatgt taatgcctgt ttcgctttcg atgttgagcc
      901 catcgtcggt ggcagaaacc tgcaaaaacg tgtctttgtt ttcagatttg aaaaggccaa
      961 tggcatcggc cacatagtcg tccagcatac aatccttgaa atacacaaca taaaattcgc
     1021 cacttttgat ttttggatga acacattgag cataaaggtg ttgtgcaaga ttgaccgatt
     1081 gctcgtaaag aacatcgggg ttttcaaaaa ttttacctgc aaaagtgtaa gcttcattga
     1141 ggtttatgtc actttcgtga aaaaagttat aatattcctg cgatttaaac gaacttctga
     1201 aatatttcac caaggcataa agcgtctttt cattagtatt tagcagcccc tttgagatag
     1261 cgcaaacttc atcgtttagc ttattcccga cataatgcac ggctatggat tgaatggcaa
     1321 aatcgtcaat ttgaatcatc tttatggcaa atgaatgttg ttgtattggg caaagttgga
     1381 caaaaacttc aaactacacc ataattgttg atacttttcc attctcaaaa tattcaataa
     1441 tattgccaat caaaattgta acatgaacca gagaaagata ctattgcagt tattgcccgg
     1501 gtttattccc ctgtttgcat ttatcattgc cgatgagttt tggggtacca aggtggggtt
     1561 agtagttgcc attttcattg gggttgttga attggcgtat tattggatta aagaccgcaa
     1621 gctcgataaa tttatactgc tcgacacctc attggttatt gcacttggtg ttatctccat
     1681 tgtgcttgac aatgaagtgt ttttcaaaat aaaacccgcg ttgataggcg tgttgatgtg
     1741 cgcaatcctt ggtgtttcgg tatatacgcc ggccaatata ttgctcaata tgtccagacg
     1801 ctatatgaag ggtattgaaa tgaatgatgc gcaatatctt cattttaaga aaaatatgaa
     1861 agtacttttc tggattttca cggcttatac ggcattggtg ttttattcgg tatggtttat
     1921 gagcaatgaa gcctgggctt tcatcagcgg aggattgttt tacatcctgt ttggggtata
     1981 ttttctttat gaaattataa aaaccaaagt ccttcagatc aagttaaaga aggaagagtg
     2041 gctgccggtg gtaaatgaaa aaggggaaat aataggcaag gctccacgga gtgcttgtca
     2101 ttcggacaaa agcttattgc acccggttgt tcaccttcat gttatcaata ataggggaga
     2161 gatatatctg caaaaacgtc ctatgcacaa aattcagccc ggtaagtggg atactgccgt
     2221 tggcgggcat atctcttttg gcgaaagcat tgaaacagcc cttgtgcgtg aagctgagga
     2281 agaaatcggg attatagggt tcaagcctaa gttggtggcc aactacattt gggagtcaga
     2341 catagagcgc gagctggtgt tttgctttgt tactcattac caaggagcta ttaatatcaa
     2401 caaagaagag cttaccgatg gccgtttctg gacgcacaat gagcttaaag atgctattgg
     2461 caaagaaata ctgacaccga attttgagat agaatatcaa aaggtgttgt caaaatttaa
     2521 ataaatactt ttttgttgaa agtatgagtt gattacttat tatgacttct aatttcgtta
     2581 aatttgcgaa atttttaaaa ttctcattat ggcacaaatt atcaatgata tttcaaggac
     2641 atttaacgaa tacttgctta ttccgggatt gactactaaa gattgtacac ccgaccggat
     2701 aactttaaaa acacctttag taaaatttgc tgcaggaagc aaagcagaaa tctcgcttaa
     2761 tattcctttt gtttcggcta tcatgcagtc tgtttcagat tctaatatgg caatagcgct
     2821 ggctcgcaat ggagggttgt cgtttattta tgcttctcaa accattgaag accaagcaga
     2881 aatggttaga aaggtaaaaa agtttaaggc cggttttgtg gtcagcgatg ctaacctgac
     2941 ccccgaaaat actttgaagg atgttataga actaaatgaa aggactggtt tttcaacaat
     3001 agctattacc catgatggca ctgccggcgg aaaacttttg ggaatggtta caagccgcga
     3061 ttatcgcccc aacaaagaca gtcttgataa gaaggtgaaa gacttcatga ccccattttc
     3121 aaaactggtt gtgggtaaat atggtatcag tcttgatgaa gccaatgaca ttatctggga
     3181 aaataaaatc aatacactgc ctatcattga tgaaaatcag tgcatgaaat attttgtgtt
     3241 caggaaagac tatgacaatc acaaagataa ccccaatgaa ctgttagaca gtcataaaag
     3301 actgttggtt ggtgcaggtg tcaatacacg ggattataaa gaacgcattc ccagattggt
     3361 agaagccggg gtagatgtgc tttgtattga ttcatccgat ggtttttcgg aatggcagaa
     3421 ggatactatt aaatttgtaa gagatacata cggaaatact gtaaagattg gtgccggaaa
     3481 tattgttgat gaagatggtt ttaattatct ggccgatgcc ggggctgatt ttataaaagt
     3541 cgggataggc ggggggtcta tttgtattac ccgagaacaa aaaggtatag gccgtggtca
     3601 ggctacttca ttaattgaag tggccaaagc tcgtgatgaa tatttcaaaa agaaaggcga
     3661 atatattcca atatgctcag atggtggtat agtgcaagat taccacatgg tgctggcttt
     3721 ggctatgggg gctgatttta ttatgatggg aaggtatttt gcacgttttg acgaaagccc
     3781 aacaaagaag ctgcttattg gtagcaatta tgtaaaagag tattggggcg agggctctaa
     3841 ccgtgcccgt aactggcagc gatatgatat gggcggcaac tcctcgctga agtttgaaga
     3901 gggcgttgat agctatgtcc cttatgccgg caagctcaaa gataaccttg acgtaacgct
     3961 gggcaagata aaatcgacca tgtgcagttg tggtgcattg tctatacccg aattgaaaaa
     4021 ggttgcaaaa atcactatgg tttcgtcaac aagtattatt gaaggtgggg cccatgacgt
     4081 tatcctgaaa gaaaccaaac gggaagactg atgaaatgtt gtgttcttaa aaaacaaaat
     4141 tcagtttttt agtttgcttt taattgcata gtgtgggttt ttaatatgat agttagctga
     4201 ggcttagagc ttggagtaga attttctttt tgtctttaga aaaatatact gctggtaaaa
     4261 ggaagaatat tttgcgatat tagtatgtta aaatgctatt acagtgtatt tttgaaaaaa
     4321 taaacattta ctatgcaacc ctggtcagaa aaaatagagc ctttgctcaa gctttatgga
     4381 aagcgaaaac atccgcttga ttatcaaaac cgctatcagt tgctggtgat ggttattctg
     4441 tctgcacgcg attctgataa gcatattaat gagctttcta aaagcttttt tgagcaatat
     4501 cctaccatga gccatctggc caaagcatcg attgttgagc tgcatcaatg tttaggtaca
     4561 ataactaatt ttgccaataa agctgagtgg ttgttaaaga tagcccaaac cattggtgaa
     4621 gataataaaa ttccggctac aatggcagaa ttgaccaaat tgccaggcat tggccgaaag
     4681 tcagcaaatg ttattattcg agaatcagga tttgaagccg aaggcgttat tgtagatctt
     4741 catgttgtaa gggttgcccc acgtcttggc attgctacag gttcgaaccc cgaaaagatt
     4801 gaaaaacaga ttatgtctgc tattcctcaa gccagatgga acgaaacggg tatggctatc
     4861 tcttttctgg gacgtgagat atgccgtcct tccaacccta aatgcgaaca atgtgtaatg
     4921 aatagttgtt gcgagcacaa cggagtgtaa ttaaatattt taaccaatct aaataaaaaa
     4981 tgaagcattt acttataatc acagctgcct gcattttagc aagccctatt tttgcgcaaa
     5041 gcaaaaaaaa taaacaagca ttgcctgaaa gagtaataaa tatcgatttc agtgcagaaa
     5101 aaggcccatt aaacactatg ttcaaaaagt gtgttggtgc agggcgcgca aatgaagggc
     5161 ttcgggctga ctggcaacaa caattggctt atgtaaaaaa agaatgcgat tttaagtaca
     5221 ttcgtatgca cgggttattg acagacgaca tgggcgttta caaagaagac aaaaacggaa
     5281 accctcagta caactatatg tatgtggatg tactaatgga ttatctgctt agcattggga
     5341 tgaagccttt tgttgaactg ggttttatgc ctaatgcatt ggccagtggt gagaaaacta
     5401 ttttttggtg gaaaggtaat gtaaccccgc caaaggatta cgacaaatgg ggtgctttaa
     5461 ttaaaaacct taccgagcat tttaccgaac gctatggtgc tgatgaggtt aaaacctggt
     5521 actttgaagt ttggaatgag cccaaccttg ttccctggtt ttttacagga acacaagaag
     5581 attatttcaa gctttacgaa tatagtgcca aagctataaa aagtgtcaat aaagattatc
     5641 gggtaggagg ccctggaact gcaggcgctg cctgggaaac agaattaatc gaattctgtg
     5701 ccaaaaacaa cttgcctatt gactttgtca gtacacactc ttatggagta aaacaaggat
     5761 accttgatga atatggtaat tcgggtacta tacttgataa aaaccctatg agtgttagtg
     5821 gagacgttat tcaaagccgc aaagaaattg ccaattcatc aaaaccaaat ttagaacttc
     5881 actataccga atggagcgct tcatataccc cttcagaccc tatccatgac agctatcacg
     5941 aagcttcgta tatacttcaa aagcttaaac aagtggggac ggcagctcaa tctatgtcat
     6001 actgggtttt tacagatatt tttgaagagt cggctcctcg ctttgaacca tttcatggag
     6061 gatttgggtt gctcaatatt cagggtatta acaaacctgc tttttatgca taccagtttt
     6121 taaaccgttt gggaaatatc gaattgacaa ataccgatac agcttcttgg gtttgtaaag
     6181 atgctaatgg taacattcaa gtattggctt gggattttac aaatacccat ccgggagact
     6241 ctgtaaataa ccaagtttat tatattcgcg acttaccttc aaagtctaaa gggaaattaa
     6301 aaatcagtat ttctaaagta cctgcaggaa tttattctct tgaagtttat aaagtaggat
     6361 accgttgcaa tgatgcatat accagctatt atgacttagg aaggccaaaa aaacttacca
     6421 ggttgcaagt tgaacaaatt aaaaaacaaa atgacggttc gcctgtagag aaggaaattg
     6481 ttaaagtaaa agaagcaata tcatttgtaa aagaattaga tatcagagag aatgatgttt
     6541 acttgctgaa gctcaccaaa ctataatttt gaaataagtt ttgaaaaata taaagcagtt
     6601 ctgtttaggg ctgctttttt tatttaaacc gcggaatgtg gattttctta acttttgaca
     6661 atacccgcaa cgaaactata agcaaaacac atatcaagat acaaacatct gggtgaatgc
     6721 caagtttaat aagcaaaata aatactaaac ctccaataaa acaagcagta gcatacattt
     6781 ctccattttt aagcaaatcg ggaatattat tacaaaggat atctgtcact aaaccaccaa
     6841 aagtagctgt aataacaccc attattactg aatataccga gccaatacca gcttgcatgc
     6901 ttatttgaat acccgcaatt gtaaacatac ctaacccaat agcatctgtg atgaagataa
     6961 cacggttaaa tttatccata ttggttttaa acaaaaaaat aatgagcact gccgaaatta
     7021 ccaatgttaa agcaaaataa tcgcccaacc agttgactgg tctaatccca agcaaaatat
     7081 ccctgatggt acctcctcca taagccgtac aaaaagccaa cacagcagca ccaaaaacat
     7141 ccattttatg agtgcgggct ttaagggttc cggtaattgc aaatacaaaa gtcccggaat
     7201 aggttataat attaagccaa ttcatttaaa atttacctta agtgtgatac agcacaaaga
     7261 acgttaaaaa aagtaaaagg tacaaacctg aaaatcaata aaaaacatgt ttaacaactt
     7321 ttatattaac tacatattta ttaacttcat ctgacagata atttaaaaat taaacatcat
     7381 agagaacccg taaatagaaa aacttaacaa taacttaata ttgaaataac atttttttaa
     7441 taaataaaaa ctactttgca aaatagtaac aattaaataa taaacaaatg tttggattag
     7501 acaatgggat ggcatttata ctaattattt gtattatagc tgcttgtttt tttgaattta
     7561 ttaacggctt tcatgatacg gccaatgctg tggcaactgt aatttacact aactcaatga
     7621 aacccggggt agctgtagtt tggtccgggg tatttaattt tattggtgtt tatatgggag
     7681 gtattggggt tgccatagga atcattaacc tgctaccaat gtcgctattg actgaccaaa
     7741 gcgtcaacca caacattgcc atgatacttt ccattattat atcttctatt atttggaacc
     7801 ttgggacatg gtatttcgga ataccttgtt caagctcgca taccttgtta ggttcgttgt
     7861 ttggtgtagg gttggctttt agtttattgc caggtgtaga aaatgttggc ctaaattgga
     7921 ctaaagtaaa agacgcagga ttgtcactaa tgctttcgcc tcttattggt tttgggttaa
     7981 ccatgggtat tgtaatttta gtcaaaaaaa tatctaaaaa gctcaagaat aacatacttt
     8041 ttgaagaacc tcaaaagaaa aaatctccac caccattagt ccgtgggctg ttagtcctta
     8101 catgtaccag cgtcagttac tctcacggtt caaatgatgg acaaaaagga gttgggcttc
     8161 ttatgattat tcttattgcg ttggccccat taagtttttc ggtcaaccat tcaatgcaac
     8221 ctgaaaaatt gttggttagt ttaaaccaga ttgacagcat tgtttctaaa atagattata
     8281 cagggcttac tgacattgaa aaaggtaccc tgacggaagc taataagcgt ttagattcga
     8341 tgcaagttat tttagcggat attaatagct ttgattcgtt aaaaaacgga cagccacagg
     8401 cattaagaaa aaatatcct
//
LOCUS       DAOSPF010000106         8337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8497_length_8337_cov_6.188119, whole genome shotgun sequence.
ACCESSION   DAOSPF010000106 DAOSPF010000000
VERSION     DAOSPF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8497_length_8337_cov_6.188119"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..312
                     /locus_tag="PK252_13770"
     CDS             43..312
                     /locus_tag="PK252_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15821.1"
                     /translation="MTINASILVEFENSQFYIEPSQGAHFFQNLTTMGTAYLTVTDNP
                     ALIMQRAVENAEKVVYETDFIKVFRFAKELVCKVDGRANKGIVMV"
     gene            370..1707
                     /gene="gdhA"
                     /locus_tag="PK252_13775"
     CDS             370..1707
                     /gene="gdhA"
                     /locus_tag="PK252_13775"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT15822.1"
                     /translation="MQADKVITALKQRYPNEPEYIQAVHEVLLSIEEVYNAYPQFEAA
                     NIIERLIEPDRILTFKVSWVDDAGHVQINRGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLAMGGAKGGSDFNPKGKSNAEIMRFCQAFMLELWRIVGPETDV
                     PAGDIGVGGREIGFLYGMYKKLTMEHNGVLTGKGTNWGGSLVRPEATGFGVVYFTNEM
                     LATIGQSIEGKKVAISGFGNVAWGAVKKATELGAKVITLSGPDGYIYDPEGMDSEKID
                     FMLELRASNEDVVKPFADRFSGAKFIDGRRPWEVPCDVAIPCATQNELDENDAKNLIE
                     NGCKCLCEGANMPCTPQAIHAIQQSKILYAPGKAANAGGVATSGLEMSQNASKLAWPA
                     NEVDMRLRQIMHNIHQNCMNYGKQPDGYTDYVKGANIAGFLKVANAMMEQGVV"
     gene            complement(1890..2849)
                     /locus_tag="PK252_13780"
     CDS             complement(1890..2849)
                     /locus_tag="PK252_13780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT15823.1"
                     /translation="MANKVSEILAASKSTRFSFELLPPLKGRSIDDIYKAIDPLMEFN
                     PININITYHQHETVYNTLPDGSLEKKIVRKRPGNVALSAAIKFKYDVTVIPHLICGGH
                     TKDDIEDLLLDFHFLGINNLLVLRGDAPKGQKVFMPEKNGHEHTTDLVKQVMNMNKGI
                     YVDPAIKDPQPTNFSVGVAGYPEKHVEAPNMETDLKHLKEKVDAGADYIVTQLFFDNT
                     KFFAFEKACRQLGITVPIIPGIKPLSTLNDIKLVPQIFSCDIPQAFYKELEKCKTNTD
                     VKQVGIEWAIHQSKELKAHNVPAIHFYTYGISDNVQQIARATF"
     gene            complement(2940..4103)
                     /locus_tag="PK252_13785"
     CDS             complement(2940..4103)
                     /locus_tag="PK252_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT15824.1"
                     /translation="MNIPLESIRTAEEKEIAALKEKLRVLEQENKTLREREFALLKAL
                     PEIVFLVDLNERFIFLNDFCRKQFEIEPDKIPYNINIKDLLSPSSIFKLRRTIIKGFE
                     ETLFHIDELKGIKSSGEQFPFTVYFSKLVEEGSLIGFIGVGFDITKIKHIETQLTEAN
                     KAKTKFLSIIAHDLRNPFNALLGFSNLLLTNYPKYSQEKVLDFVKHISNAANQGYQLL
                     ENLLEWTRANAGKIEIIPETFDISKIIVETSHIIEEVANKKEIQILHNYSSPLKVTAD
                     KNMVSTIIRNLLSNAIKFTYRKGKIVIRVLQDTEYAIVEVKDNGVGIPAENLKNLFDI
                     SIETSSLGTEREKGTGLGLNLCQEFAFLNKGKIEAESIVGQGSTFRLYLPLFH"
     gene            4319..5902
                     /locus_tag="PK252_13790"
     CDS             4319..5902
                     /locus_tag="PK252_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15825.1"
                     /translation="MKGFKNRLIIVQLFFLLGTNLHAERVIRQFVKKDYLYFSDTIEA
                     ALFDTSRKMDLRQYFLSAIYTKGSIAGPLDAENAYSRFLQYIDGIRKKNVMALKPEKL
                     VKVLYKETHNKFFKKYEINPEFEDIFRNGDYNCVTATLLYALLFDTLNIDYQIKESAV
                     HVFLVALPKNKSIAIESTDPQKGYLVLNESFKNKFVDYLLAQKLVTLSEISQEGKDAV
                     FDKFYYARININIQQLIALLYYNNGVKAIDNEDYHSALMFFLKSYYLYPSEKSKYLIL
                     SCILNELNKANYTRQEDWRNYVILSNFVGSLISVDNYCDEFLRITNRLLIEKTDTFNY
                     TKVFQYINQHVTDTTVLKKLSYIYHYESGRMYLANKDYARAYPYFEQAFLLQPENYQV
                     QNIFFETFTAALQTMNEDTAMAKLEQTVDLYGEKLVNTKMYYFLAAAYLYKAQLAYTH
                     QKIKEGDDYLLKFENTYLKYNIGQQFYELIGEAYTKPAALFFKRNNVKKAREYIERGL
                     KLAPGHYRLTNSLKMLKQP"
     gene            complement(6041..6892)
                     /locus_tag="PK252_13795"
     CDS             complement(6041..6892)
                     /locus_tag="PK252_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15826.1"
                     /translation="MTSLKKSNKDVFSLLAKLSAKKLTISIALAFIVIFLAQCNLSKD
                     NPEGYVEEYYSTGVLKTSYTIKDGKLNGVVKNYNEKGRLISTVEYKDDKKNGKLVNYN
                     SKNGKPMIVAYFNDNIQEGPVTQYYDEGQLFRTSTYLNGRINDTVKTFWPNGKIKAIN
                     VYKMGMPAIGLKEYDKNGQLLKQPQLVIQEIDQTALLNKVILKISVSGDWENVDYYEA
                     NLVEGKYFQESAINALMDKNGVVTIDYPAPKGNTYMEKLSIVAKVKTRYGNTLILHKY
                     HNLSIMN"
     gene            complement(7295..8170)
                     /locus_tag="PK252_13800"
     CDS             complement(7295..8170)
                     /locus_tag="PK252_13800"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOT15827.1"
                     /translation="MKMILNLANKFLKYFCLALVLLVNHSAHAEDYAIQLLEQMRQKA
                     NEVKTLRYDAVMNERIKGKMVEKVSSFKINTSPEKIYVAQSFIGIKLDALYCQGWNNN
                     QLRVATVGFPWLELNLDPLGSMVRNNHHHTIFEAGFTYFVNIIGQIVDDPNRQLTITY
                     MGNVKRNGISCQRINICNKKYAIIKYQIKAGENLTTIAKRLNINDYKILELNPEIDDY
                     TDVKPGQIINIPNTYAKSLDLFIDETIMLPVLIDIYDENGQYGSYGYKNLVINKPFAA
                     DEFSITYKDYHFKKK"
BASE COUNT         2479 a         1375 c         1711 g         2772 t
ORIGIN      
        1 tttatctttg caattaagtt atcataaaca accttgtgaa acttgacaat taatgcttct
       61 attcttgttg aatttgaaaa tagtcagttt tacattgagc caagccaggg ggctcatttc
      121 tttcagaatt taactacaat gggaacagct tatttaacgg tgacagataa tccagcgctt
      181 attatgcaac gagctgtcga aaatgctgaa aaagttgttt acgaaactga ttttataaaa
      241 gtttttcgct ttgccaaaga gctggtttgt aaagtcgatg gcagggctaa taaaggaata
      301 gtaatggtgt aacctcccca ccccccctcc cttttataag gaggggtagg ggtgctaaaa
      361 tatatagtta tgcaagcaga taaagtaatt acagcgctta agcaacgtta tccgaatgaa
      421 cctgaatata ttcaggcagt tcatgaggtg ttgctttcta ttgaagaagt ttacaatgct
      481 tatcctcagt ttgaggcagc aaacattatc gagcggctta ttgagccaga taggatactt
      541 acttttaagg tttcgtgggt ggatgatgcc gggcatgtac agataaaccg cggatatcgg
      601 gtgcagttta ataatgctat tggcccttat aaaggcggat tgaggtttca cccgagtgta
      661 aacctgagta tattgaagtt tcttggtttt gaacaaattt ttaagaatag ccttactacg
      721 cttgctatgg gtggggcaaa gggcggctcg gattttaatc ctaaaggaaa atccaatgca
      781 gagattatgc gtttttgtca ggcctttatg cttgagctat ggcgtattgt tggtccggaa
      841 actgatgtgc cggctggcga tataggtgtt ggtggccgcg aaattggctt tttgtatggt
      901 atgtataaaa aacttaccat ggaacacaat ggagtgttga caggtaaagg gacaaactgg
      961 ggaggttcgc ttgtgcgtcc tgaggctact ggttttgggg tagtttattt taccaatgaa
     1021 atgttagcaa caataggtca aagcattgaa ggaaaaaagg ttgccatatc aggatttggc
     1081 aatgttgcct ggggggctgt taaaaaagcc actgaattag gggcaaaggt cattacactt
     1141 tctgggcctg acggctatat ctatgacccg gaaggtatgg attccgaaaa gatagatttt
     1201 atgctcgaat tgcgagccag caatgaagat gtagttaaac cttttgccga caggttttca
     1261 ggtgcaaagt ttattgacgg taggcgtcct tgggaggtgc catgtgatgt ggctattcct
     1321 tgtgcaacac aaaacgaact ggatgagaat gatgcaaaga acctaattga aaatggctgt
     1381 aaatgcttgt gtgaaggggc aaatatgcct tgtacccctc aggcaataca tgctattcag
     1441 cagtcgaaaa tactatatgc tccgggaaag gctgcaaatg caggaggtgt agctacgtca
     1501 ggccttgaaa tgtctcagaa tgcttcaaaa ctggcatggc cggcaaatga agtggatatg
     1561 cgcctgcggc agattatgca caatattcat caaaactgca tgaattatgg caaacagccc
     1621 gatggataca ctgattatgt taaaggggct aatattgcgg gattccttaa agtagccaat
     1681 gctatgatgg aacagggagt ggtgtagtgg tgtagtggtg taggttgtcc aattaagccc
     1741 caatcttaat catcaagagc aactttttgc cgatgaatat gatatttagt gggttagctc
     1801 tcttgttatg gaccagacaa gctgattttg aaattgttta atgtaattta ctaactattc
     1861 ggccttaaag caataaacag gcgctaagat taaaaggtag cccgggcaat ttgttggaca
     1921 ttgtcagata tcccgtaagt ataaaagtgg atagcaggta cgttgtgggc ttttagctct
     1981 ttgctttgat gaatagccca ttcaatgcct acttgcttta catcagtatt agttttacat
     2041 ttctcaagtt ctttgtaaaa ggcttgaggg atatcacaac taaaaatctg gggcaccaac
     2101 tttatatcgt taagtgttga aagcggcttg atgcccggaa taatcgggac ggtgatccct
     2161 aattggcggc aagctttttc aaaagcaaaa aatttggtat tgtcaaaaaa taattgggtt
     2221 acgatgtaat cagccccggc atctactttt tctttcaggt gtttgaggtc tgtttccata
     2281 ttgggggctt caacgtgttt ttcgggataa ccggctacac ctacactaaa gttggtaggc
     2341 tgtgggtctt tgatggctgg gtcaacataa atacctttat tcatgttcat tacctgtttt
     2401 accaggtctg tggtgtgctc gtggccattt ttttcgggca tgaatacttt ttggccttta
     2461 ggggcatcgc cacgcaacac taaaaggttg ttgataccaa gaaaatgaaa gtcgagcagt
     2521 aaatcttcaa tatcgtcttt ggtatggcct ccgcaaatga ggtgaggtat tacagttaca
     2581 tcatatttaa atttgatggc tgctgataaa gcaacattac ccggacgttt gcgaacaatt
     2641 tttttttcta aagagccatc aggcaaagta ttgtaaacag tttcgtgctg atggtaggtg
     2701 atgttaatat tgatggggtt gaattccatc agggggtcta tggctttgta tatatcatca
     2761 atactgcgtc cttttaaagg tggaagtaac tcaaatgaaa accgggtact ttttgaagct
     2821 gcaagtattt ctgaaacttt gtttgccatt gtattttgtt tttaggttgc aatattatga
     2881 catcgcggca taatatgcaa atgttgatat tttcaaaggc tctttgacag aagggtaatt
     2941 taatgaaaaa gcggcaggta taacctgaag gtacttcctt gaccaacaat gctttcggct
     3001 tctattttcc ctttattgag gaacgcaaac tcctggcaaa gattgagccc cagtccggta
     3061 cctttttcgc gttcggtgcc taaacttgaa gtttcgatag aaatatcgaa aaggtttttt
     3121 aagttttctg cgggtatgcc tacgccgtta tctttcactt ctacaatggc atattcagta
     3181 tcctgtaaga cacggattac aatcttgcct tttcggtatg taaatttgat ggcattgctc
     3241 aacaagttcc tgatgatggt gcttaccatg tttttgtcgg cggttacttt tagcggcgaa
     3301 ctataattgt gaagtatttg tatttctttt ttgttggcaa cttcctcaat gatatgcgat
     3361 gtttctacaa ttattttaga aatatcaaaa gtttcaggta ttatttcaat cttgccagca
     3421 tttgccctgg tccattccag caggttttca agcaactgat acccttgatt ggcagcatta
     3481 gatatgtgtt taacaaaatc aagtaccttt tcctggctgt atttggggta attggtaagc
     3541 aataggttgc taaatccaag taatgcgttg aacggattac gtaaatcgtg tgcaatgata
     3601 gataaaaact tcgttttagc cttattggct tcggtaagct gagtctcaat atgttttatt
     3661 ttggtgatat caaagcccac gccaatgaac ccaatcaacg atccttcttc aaccagtttt
     3721 gagaaataca cagtaaatgg gaattgttcg ccactgcttt taatgccttt taattcgtca
     3781 atatgaaaca gtgtttcttc aaagccttta ataatggtac gccttaattt gaaaatactt
     3841 gaaggtgaga gcaggtcttt tatattgata ttgtaaggga ttttgtcggg ttcaatctca
     3901 aattgcttac gacaaaaatc gttcagaaaa ataaaacgtt cattaaggtc aaccaaaaat
     3961 acaatctcag gaagagcctt aagtaaggca aattcacgtt cgcgaagagt tttattctct
     4021 tgttccagaa cccttagctt ttctttcaac gctgcaattt ccttttcttc agcagttctt
     4081 attgattcaa gaggaatatt cattaaacat tatttataca ggggttaata ctgcaaattt
     4141 tactctgttt tttttaataa aaaatatttt ttgattaaca tttaattaaa aagtagcaat
     4201 tgattgtcaa tgatatatac gatttttttt atagtttgtt ttgattaatt ttgaaatcaa
     4261 agattttgga aagcttaggt ttgttattat tcaataaaag gttggtaaaa tttttcggat
     4321 gaaaggtttt aagaatagat tgattatagt tcagttgttt tttttgctgg gaacaaacct
     4381 tcatgcagag agagttatta gacaatttgt aaaaaaagat tatttgtact tttcagatac
     4441 aattgaagcc gctttgtttg atacatcgcg caagatggat ttgagacaat attttttatc
     4501 agctatttat acaaaaggct ccattgcagg gcctttggat gccgaaaatg cttattcccg
     4561 gtttttgcaa tatatagatg gaatacggaa gaaaaatgta atggctttga agcctgagaa
     4621 gcttgtaaaa gtgctttata aggaaacaca taacaagttt tttaaaaaat atgagataaa
     4681 tccggagttt gaagatattt ttcgaaatgg agattacaat tgtgttacag ctaccttgtt
     4741 gtatgctttg ttgtttgata cgttaaatat tgattatcaa ataaaagaaa gtgctgttca
     4801 tgtttttctg gtagctttac ccaaaaataa aagtattgca atagagtcca ccgatcccca
     4861 aaaggggtat ttagttttga acgagagttt taaaaataaa tttgtggatt accttttggc
     4921 ccaaaaattg gtgacccttt cagagatttc gcaagaaggg aaagatgctg tgtttgataa
     4981 attttactat gcccgcataa atattaacat tcagcagcta atagctttgc tttactacaa
     5041 caatggggtt aaggctatag ataatgaaga ttaccatagt gctttaatgt tctttttaaa
     5101 atcatattac ctatatccat ccgaaaagag caagtacctt atattgtcat gtatattaaa
     5161 tgagttgaac aaggcaaact atacccggca agaggattgg agaaattatg taattttgag
     5221 caactttgta gggtcattga taagtgtaga taattattgt gacgaatttt tgaggataac
     5281 caatcggtta cttattgaaa aaaccgatac ttttaattat acaaaagtat ttcaatacat
     5341 aaaccaacat gttaccgata caacggtttt aaaaaagctc agttatatat atcattatga
     5401 gtcagggcgg atgtatttgg ccaataagga ttatgcaagg gcttaccctt actttgagca
     5461 ggcttttttg ctacagccgg agaattatca ggttcaaaat atcttttttg aaacctttac
     5521 agctgcattg caaaccatga atgaagatac ggctatggcc aaactggagc aaactgtaga
     5581 tttatacggc gagaaacttg ttaataccaa gatgtattat tttttggccg ctgcatactt
     5641 atataaagca caactggctt atacgcacca aaagatcaaa gagggggacg actatctgct
     5701 aaagtttgaa aatacttacc tcaaatataa tattggacaa cagttttacg agctgatagg
     5761 cgaagcttat accaagcctg ctgcattgtt ttttaaacgc aacaatgtca aaaaagctcg
     5821 agaatacatt gaaagaggat tgaaactggc cccggggcat tatcggttaa ctaattcgct
     5881 taagatgcta aaacagccat gatattggcc tgcagcttgc gagctttgcg aagcttagca
     5941 caaatcaatc ctgataaatc gagatagtgt tctcccgtta aagacgggag aagctgttat
     6001 ttcttaaaaa ttaaattctg tttggatgaa gtaaacgata ttagttcatt atggatagat
     6061 tgtgatattt atgtagtatc agcgtgttgc cgtaccttgt ttttactttg gctacaatgc
     6121 taagtttttc catgtaagta ttgcctttag gtgccggata atcaatagta acaaccccat
     6181 ttttgtccat aagggcattt attgcgcttt cctgaaaata tttaccttct actaaattgg
     6241 cttcataata gtcaacattt tcccaatccc ccgaaacact aatttttagt ataactttat
     6301 taagcaaggc ggtttggtca atttcttgta tgacaagctg gggctgtttg agcaattggc
     6361 catttttatc atactcttta agcccaatag ctggcattcc cattttataa acattaatgg
     6421 cttttatttt tccattaggc cagaaggttt ttaccgtgtc attaattctg ccattgaggt
     6481 atgtacttgt cctgaatagc tggccttcgt cataatactg agtaaccggg ccttcttgta
     6541 tgttgtcgtt aaaataagct acaatcatgg gcttcccgtt tttagaattg taattgacaa
     6601 gtttgccatt ctttttgtca tctttatact ctacagtaga tattaatcgg cctttttcgt
     6661 tatagttttt tacaacacca ttgagtttcc catcttttat ggtgtatgaa gttttaagca
     6721 cgcctgttga atagtattct tctacatatc cttcggggtt gtcttttgac aaattgcatt
     6781 gagcgagaaa aataacaatg aatgccaatg caatacttat ggttagtttt ttggcgctta
     6841 gtttggccaa caaactaaaa acatctttat ttgatttttt gaggcttgtc atatttttat
     6901 ttgtgtaaaa ataacaattt cttacagcaa aagttatttt atagaaatag aattttcccg
     6961 gaattagttt cagcgcttat ctccggtatt tagtatgact atactaaaaa gatttacccc
     7021 cactgttgtc cggtaaaata aaatagatac ttataaatat tagtaatatc gttgattaga
     7081 aacaggtagc cccgttgggg cttaatttta aggatgttta attttactac aaacaggacg
     7141 tcgctacgcg actaaataat agcagcgtag ctgcgaactg tttgtaggat gaccaaatac
     7201 accaaatgat tctaagctgc atagcagcga cctgtttttt accggacaac aatggtttac
     7261 ccctaaggct ttcaaagtat tgtaaaaaaa ttttctattt ctttttaaaa tggtagtctt
     7321 tataagtaat agagaactcg tcggctgcaa aaggtttgtt aataaccagg tttttatagc
     7381 cataagaacc atattggccg ttttcgtcgt aaatatcaat aagtacaggg agcataatag
     7441 tttcatcaat gaataaatca agtgatttgg cataggtatt tgggatatta attatttggc
     7501 caggtttgac atcggtataa tcgtcaattt ctggatttag ctcaaggatt ttatagtcat
     7561 tgatgttgag ccgttttgct attgttgtca ggttttctcc ggcttttatt tggtatttga
     7621 tgatggcata ttttttattg caaatattga tgcgctggca tgaaatgcca tttcttttta
     7681 catttcccat ataagtaata gttagctgac ggttggggtc gtcaacaatt tggccaataa
     7741 tattgacgaa atacgtgaac ccggcttcaa aaatagtgtg gtggtggttg ttacgtacca
     7801 tactccccaa agggtcgagg ttcaattcga gccagggaaa gcctactgtt gccacccgaa
     7861 gttggttgtt gttccagccc tggcaataaa gggcatcgag cttaatacca ataaaagact
     7921 gggctacata tattttttcg ggcgaggtgt taattttaaa actggatact ttttctacca
     7981 ttttaccttt aatacgttcg ttcattactg catcatagcg aagggttttt acttcattgg
     8041 ctttttgacg catttgttca agcagttgaa tggcatagtc ctcggcatgg gcactatggt
     8101 taactaataa taccaatgca aggcaaaaat attttaagaa tttatttgct aaattaagaa
     8161 tcatcttcat ggtagaaatt ttgaggtgca aagttaatcg ttaaaaataa tacaagtgtt
     8221 aaaaaaacaa aatggcaaaa ttttgctttc ctattttcct gataataact aatattgcaa
     8281 cgtcattact gtcccattaa aatctaaaag agttctttga aaagtttgaa atttagt
//
LOCUS       DAOSPF010000107         8135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8808_length_8135_cov_7.727847, whole genome shotgun sequence.
ACCESSION   DAOSPF010000107 DAOSPF010000000
VERSION     DAOSPF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8808_length_8135_cov_7.727847"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..411)
                     /locus_tag="PK252_13805"
     CDS             complement(97..411)
                     /locus_tag="PK252_13805"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT15828.1"
                     /translation="MKKYKVKIEPEALADIQEITDWYNEAQAGLGKRFQNTAIKQINS
                     LNKDPQIYAIRYKEIRCVVIKKFPYMAHFFINDENNTVEVLAVISTDRNPKIWKEKTS
                     EH"
     gene            complement(411..644)
                     /locus_tag="PK252_13810"
     CDS             complement(411..644)
                     /locus_tag="PK252_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15829.1"
                     /translation="MNVQAQKAIIIEQFKQVDDVNLINAIKNMLDYALKKEKESIDIP
                     EAHQKLVMERFNKVRKKPERLLDWDEAKKTLKA"
     gene            complement(663..995)
                     /locus_tag="PK252_13815"
     CDS             complement(663..995)
                     /locus_tag="PK252_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT15830.1"
                     /translation="MLFGDRLSEIRKKKKISQDDLAKKIGVHAPVIGRYERSDVKPSI
                     EVAVKITEALNVSLDYLVGSTDLILEKGIVNRIQSNQKLSKDDKGHLFALMDAFLLKC
                     NIQSNLAV"
     gene            complement(1570..2538)
                     /locus_tag="PK252_13820"
     CDS             complement(1570..2538)
                     /locus_tag="PK252_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15831.1"
                     /translation="MKNFMKTSLAVALSAMAVITVSCSKDEDESYVDSYSPTLTEAKG
                     TAYTANDYFPLVKNNSYMYSANLVITGTETTNGTKETINEKDTSYSYAYVKGKEKVTF
                     ESGTKEVITYYNNGDEVYYQINDNGIVCVGQKSNGSIIEFKNNTVFLKKSLEVGDSWP
                     YNPTDDYSELFSSWGVSASDMKYTAKIFVVGKESFKLNDTSVTALRMDQVIEFSVNMS
                     ESENGVSYKITMSMKMTAPNYYAKGVGLVKQKVDATIKMEMSASGNGRSASASVEANI
                     KGTNELSYFSKGLTSKKSSVEPGSNVFSVIQGMKAQLLEKCKKNIK"
     gene            2705..3652
                     /locus_tag="PK252_13825"
     CDS             2705..3652
                     /locus_tag="PK252_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOT15832.1"
                     /translation="MNRIDRLVAILTFMQSRRLVKASDIAERFDISLRTVYRDVRALQ
                     ESGIPVCGEAGSGYTVEAGYHLPPVMFTREEAAAIFTGAKMIDKYTDLSVNKHFCTAL
                     EKVKAVIRYSDKEYLEKIDNQILVLRPPSEDSEFPNCFMVTVQKAIAESHIVNIEYFS
                     GYKKETTRRKVEPIGVCFYSARWHLFGYCKLRNEYRDFRLDRIKQLEITDEKYTSAHP
                     TVQELIPKLFDNSAYLKVSLLFPFDMQAKLAEQKYYYGFIEEVLTPEGILMHFMTDSY
                     EWIERWVLSLGKNVTIVGPQAFKDLVKSKVKALTEHYQG"
     gene            3909..4361
                     /locus_tag="PK252_13830"
     CDS             3909..4361
                     /locus_tag="PK252_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOT15833.1"
                     /translation="MEVKICNPLKTLSFSVETTFNEMMPLVGSVARELCAEAVANKLD
                     ITGPVYWVYEGADGNPDTRFKLDICLPVFAPGGYSGKFKFVELPAFKCASAMHYGHWN
                     EVGAIYHHLFDRLAEEKLEYNGVCREIYINSDFADPANNITEVQVGVN"
     gene            4488..4913
                     /locus_tag="PK252_13835"
     CDS             4488..4913
                     /locus_tag="PK252_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15834.1"
                     /translation="MKKLILPSFLAAIASFAMSIAIGFAFMFIFPSLNDEYSNTSIFR
                     TWEDPLMYIYFLQPFFFAFTLAWIWTKVKKLFAENIMNAAFTLTGAYWLVATIPGMLI
                     SYSSYQISSAMVLSWTVSSFFQTYVASLVIIKMEKRYTA"
     gene            4925..5797
                     /locus_tag="PK252_13840"
     CDS             4925..5797
                     /locus_tag="PK252_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT15835.1"
                     /translation="MARYIQAKTILSTVKHKPDPYFGLSYNMNLYRGCQHGCIYCDSR
                     SKCYQLGELSDIRVKENAINMLECELRSKRTIGTIGFGSMNDCYMPVEKEYQLTRKAL
                     EVIKRYHYPIHIITKSNLVVRDIDLLREINKVYAAVSFTITSEDDNLCKTIEPCAPTT
                     SERFEAMSKLSSEGIYCGVTLMPVLPFINDTVENVVSIVEKAKAAGAKYILAVFGLTL
                     REGNREYFYDALDKLFPGIRARYEKVFGMQYNCSSPNSSELYKTFNRLCNEAGIATKM
                     EFYKPAQIVELKLF"
     gene            5800..6150
                     /locus_tag="PK252_13845"
     CDS             5800..6150
                     /locus_tag="PK252_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT15836.1"
                     /translation="MENIKNDTKLIAYCGLYCGACRLYISGKCPGCADNAKATWCKIR
                     SCCIENNYQSCADCKQFANPMDCKKFNNFISKVFGLIFRSDRAACIAKIKQDGPTAFA
                     AFMSSNGWQSIKRK"
     gene            complement(6425..7948)
                     /locus_tag="PK252_13850"
     CDS             complement(6425..7948)
                     /locus_tag="PK252_13850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOT15837.1"
                     /translation="MAENKEISDLRRELETLRLENAHLKLVNRSLNSVVENSPASIVI
                     TNKQGVIEYVNPIFTKITGYTAEEAIGMHTRVFKSGYHPDEYYKAMWHTISSGKTWKD
                     AFKNRRKDGSLYWEQQFITPIINDNNEISHFAAIKIDISLQKEYEEKLRKSQQYLDTL
                     TKALPELILVLAEDGFLIDAITNEKLEAYKILLQAKSSNIFNVLPVDLHTILRDLIER
                     TIKSNSIQIAEFPIEHRGKVLWFEARSVLLDYEIKENLNSIVAVIRDITVRKNAEQSL
                     IELNATKDKFFSIIAHDLRNPFNALLGISEIMYNSDEIDVQQMIEMSKLINESSRNAY
                     ELLENLLQWSRSQTGQIKFEPNYHNLYQIVEQGIKIIEGQAFSKNVKVLNRIPGNEVV
                     FADVNLLKTVIRNIVSNSIKYSYPHSEVLIYSESDKEMTKIVVSDMGKGMKPSTLQNL
                     FKIDQKISIPGTNNEKGTGLGLILCKEFIDLHNGFINVYSEPEAGTNFKISLPLPVR"
     gene            complement(8056..8131)
                     /locus_tag="PK252_13855"
     tRNA            complement(8056..8131)
                     /locus_tag="PK252_13855"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8094..8096),aa:His,seq:gtg)
BASE COUNT         2413 a         1619 c         1498 g         2605 t
ORIGIN      
        1 tacctacatg aaacctttat gggtattgaa attattctaa aagtaggttt ataattgaaa
       61 tgcctggaaa ttcaaagttc cgggcttttt tacttattaa tgctcgctgg ttttttcttt
      121 ccagatttta gggttacggt cggtgctgat tacggctaaa acttcaaccg tattgttttc
      181 atcgttaatg aagaaatgcg ccatgtacgg gaatttctta ataaccacac agcgaatttc
      241 cttataacgg atggcgtaaa tctgagggtc tttgttcagg gagttaatct gctttattgc
      301 tgtattctgg aaccgtttac caagacctgc ctgagcttcg ttgtaccagt cggtaatctc
      361 ctggatatcg gcaagggctt ccggttcaat ttttactttg tactttttca tcaggctttg
      421 agcgtttttt tagcttcgtc ccaatccaaa agcctttcag gttttttcct taccttgtta
      481 aagcgttcca ttaccagttt ttggtgggct tccggaatat caatgctttc cttttctttc
      541 tttaaagcat agtcgagcat gttttttata gcattaataa gatttacatc gtctacctgc
      601 ttaaactgct ctataataat cgctttttgt gcttgtacgt tcatgttact tgttttaact
      661 ggttaaacag ccaaattact ttgaatattg cattttaata aaaatgcatc catcagggca
      721 aacaaatgcc ccttgtcgtc cttgctaagc ttctggttgc tctggatacg gtttacaata
      781 cctttctcca atattaaatc agtactgcct acaagatagt ctaaagaaac atttaaagct
      841 tcagtaattt taacagccac ttcaatagaa ggttttacat cgcttctctc gtaccttcca
      901 atcacaggag catggacccc gattttttta gcaaggtcgt cctgcgatat ttttttcttc
      961 tttctaatct cagaaagtct gtctccgaac aacataaatg tgttttttag tactgtaaaa
     1021 tcaatgtatc cacaaaaata caaaacaaat gttgtctaat caagtaataa tttatttgga
     1081 agttaaaaac agatattctt ttggaacatt tttgttcccc cttatggcgc gagtctgcga
     1141 cttgtgccta ctattcttgc agtttgcaac tgctatataa gaaggcagat gtagtagggg
     1201 ttaaatttta ctatattgta attgtaatat tttgtagacc aaatcaaagg cttgaattat
     1261 ttaaggggat aactgagttt taaacttcaa taaaaagggg cacaagtcgc aagcttttgc
     1321 cataaggcgg agaaatatga taagaaaggc tatctttaag aatatctaag cgatttaagt
     1381 ctgacatcga ccaaacctgc ccaattatct gggtcttatt ttgtattaga attgctgcat
     1441 cctactaaaa acacaatcat tagtagaatt aatagatttt tttcttcatt atttttaaag
     1501 ggtacaaaga ataaaaaaaa gcctgcacgt tcaaacagca ggcttttcat aaggctattt
     1561 gatcaaatgt tatttgatgt tcttcttaca tttttcaagc aattgggctt tcatgccttg
     1621 aattactgaa aatacattgc tgccaggttc aacacttgat ttttttgagg taaggccttt
     1681 gctgaaatat gaaagttcat tagtgccttt aatattagct tccactgaag ctgaagctga
     1741 tcttccgttt ccagaagcag acatctccat tttaattgtg gcatcaactt tttgtttaac
     1801 caaacctact cccttagcat aataatttgg agctgtcatt ttcatagaca tagttatttt
     1861 atagctgacc ccgttttctg attcagacat attcactgaa aactcaataa cctgatccat
     1921 cctgagagct gtaacacttg tatcgttaag tttaaagctc tctttaccaa caacaaagat
     1981 tttggcagta tatttcatgt cagaagcaga aacaccccaa ctcgaaaata attctgaata
     2041 atcatctgtg gggttgtaag gccagctatc gcctacttct aagcttttct tcaaaaaaac
     2101 agtgttgttt ttaaattcaa ttatactgcc attagacttc tggccgacac aaacaatgcc
     2161 attatcattg atttggtaat aaacttcatc tccattatta tagtatgtaa tcacttcctt
     2221 agtgccggat tcaaaagtga ccttctcttt ccctttgaca taagcgtaag aatatgaagt
     2281 gtctttttca ttgatagttt ccttcgtccc gtttgttgtt tcagtaccag ttataacaag
     2341 attggcagaa tacatataag aattgttttt caccaaaggg aaataatcat tggctgtgta
     2401 tgctgtccct ttagcttcgg taagcgtggg gctgtaacta tccacataac tctcatcttc
     2461 atctttactg cacgaaacag taataacagc cattgctgac aacgcaacag ccaaactggt
     2521 tttcataaaa tttttcattg gtttaaattt tgattaatat ttgtgtttga aaatctaaaa
     2581 acaagtatgt tttatgtcaa agttaaccat ttccatcaga attttggaat tttctgcaaa
     2641 tttgaaattg ttttatctca aattgccatt catcttatat ttgtttgttt tttaaaaatt
     2701 ctgtatgaac cgcattgacc gccttgtagc tatcctcaca tttatgcagt cgcgacgact
     2761 ggttaaggcc agcgatattg ccgaaaggtt tgacatcagc ctgcgaactg tttatcgcga
     2821 tgtacgcgct ctgcaagagt ccggcatacc tgtttgtggc gaagccggat cgggatacac
     2881 tgttgaggcc gggtatcatc tgcctccggt catgtttacg agggaagaag ctgcagccat
     2941 cttcaccggg gcaaaaatga tagataaata caccgacctc tcagtaaata agcatttctg
     3001 cactgccctc gaaaaagtaa aagcggtaat aaggtatagc gacaaagaat atcttgaaaa
     3061 gatagacaac cagatacttg tattgcgtcc accttctgaa gattctgaat ttcccaactg
     3121 tttcatggtt accgtgcaga aagccattgc agaaagccat attgtcaata tcgaatattt
     3181 ttcagggtac aaaaaagaaa ccacccgacg aaaagtcgag cccataggcg tatgcttcta
     3241 tagtgcccgc tggcacctgt ttggctactg caaactacgc aatgagtatc gcgatttcag
     3301 gctcgaccgc atcaaacagc tcgaaatcac tgatgaaaaa tacacctcag cacatcccac
     3361 tgttcaggag cttatcccta agctttttga caactcggct tacctaaaag tcagcctgct
     3421 attccccttc gacatgcagg ccaaactggc tgaacaaaag tactattacg gtttcattga
     3481 agaagtcctt acccctgaag gaatactaat gcactttatg accgattcgt acgaatggat
     3541 tgagagatgg gtgttatcac ttggcaagaa cgtaacaatt gttggccctc aggcattcaa
     3601 agatttggta aaaagcaaag ttaaagcatt aacagagcat tatcagggtt gaaatatttc
     3661 ataaaatatc ctttcaaaac ctattcatcc aaggtagcgc atagcctaag ctgtcaagca
     3721 aaaaaataag tgtcagaaat ttaaccctta cagggctttt ggcgcactag ctgccttgac
     3781 aagaagagct ttcccgccgc tggccgggga tagttctata aaatttttta gaaaactatt
     3841 tcctgctgac ataaggttgt cactccgccc tgttttcttt gttccataat catttaaaaa
     3901 caatcattat ggaagttaaa atttgtaacc ctttgaaaac acttagcttt agtgttgaaa
     3961 ctacttttaa cgagatgatg ccgcttgttg gctctgtggc ccgcgaacta tgtgccgaag
     4021 ctgtagcaaa taaattggac atcacaggcc ctgtatattg ggtctatgaa ggtgctgatg
     4081 gcaatcccga cacacgcttt aagctggata tctgcctgcc tgtatttgcc ccaggtggtt
     4141 attcaggaaa atttaaattt gttgagctcc ctgcatttaa atgtgcaagt gccatgcatt
     4201 acggccattg gaatgaagtg ggcgctatat atcaccactt atttgacaga ctggccgaag
     4261 aaaaacttga atacaacggc gtttgtcgcg aaatatacat caactccgat tttgccgacc
     4321 ctgctaataa tattactgag gtgcaggttg gagtgaatta attgaaaatt attaatgaaa
     4381 cgagcatgat tcgttaaaca caaacaagga tgaaaattca tcatgagagt tttccttcgg
     4441 agagcctttc ggggttcatc cgaccttatt tataaaatta tttacagatg aaaaaactta
     4501 tcttaccttc atttcttgca gcaatagcca gctttgcaat gagcattgcc attggctttg
     4561 ctttcatgtt tatattccca tcgcttaacg acgaatatag caacaccagc attttcagga
     4621 catgggaaga tccgctaatg tacatttatt tcttgcaacc tttctttttt gcatttacct
     4681 tagcatggat ttggacaaaa gtaaaaaaac tgtttgccga aaatatcatg aatgctgcat
     4741 ttactctgac aggcgcgtat tggcttgttg caacaatacc aggaatgctc atcagctata
     4801 gctcttacca gatttcatca gctatggtgt taagctggac ggtgagcagc tttttccaaa
     4861 cttatgttgc ttccttggta atcattaaaa tggaaaaacg atacacagcc taacaaacaa
     4921 aataatggca cgttatatac aagctaagac aatactttcg acagtaaaac ataaaccaga
     4981 cccttatttc gggcttagtt acaatatgaa cctctatcga gggtgccaac atggctgtat
     5041 atactgcgat tcgcgcagca aatgttacca gctgggcgaa ctttcagata taagggtcaa
     5101 agaaaatgcc attaatatgc tcgaatgtga gttgcggagc aaaagaacca ttggtaccat
     5161 tggctttggc tcgatgaacg attgttatat gcctgtagaa aaggaatatc agcttacccg
     5221 taaagcgctg gaagtcatca aacgttatca ctatcctatt catattatta ctaaaagcaa
     5281 cctggtagtc agggatattg atttactcag ggaaataaac aaggtgtatg ctgccgtgtc
     5341 ttttaccatt acatctgaag atgacaatct ttgcaaaact attgaaccat gcgcccctac
     5401 aacatccgaa cgctttgaag ctatgagcaa actgtcatcc gaaggtattt actgtggagt
     5461 taccctaatg ccagtacttc catttatcaa tgacacggtc gaaaacgtcg tttctattgt
     5521 tgagaaagcc aaagccgcag gggcaaaata tattctggca gtctttgggc ttactttgcg
     5581 tgaaggaaac cgcgaatact tctacgacgc tttggataag cttttccctg gcatcagggc
     5641 ccgctatgaa aaagtgtttg ggatgcaata taactgttca tcgcccaatt catctgaact
     5701 atacaaaaca tttaaccgct tgtgcaatga agccggcatc gcaacaaaaa tggaatttta
     5761 caaaccggct cagattgtag aacttaaact tttttaatca tggaaaatat aaaaaacgac
     5821 acaaaactga ttgcttactg tggattatat tgcggcgcct gccgtttgta tatatcaggc
     5881 aaatgccccg gctgtgcaga caatgccaaa gctacctggt gcaaaatccg ttcttgctgc
     5941 atcgaaaaca actaccaaag ttgcgccgac tgcaagcagt ttgccaaccc tatggattgt
     6001 aagaaattca acaactttat ctcaaaagtt tttgggctta tctttcgctc cgaccgtgca
     6061 gcttgcattg ccaaaatcaa acaagacggc ccgactgctt ttgctgcatt tatgagtagt
     6121 aatggctggc aaagtattaa aagaaagtag aaacaagctt tataaaaaac gctttgacct
     6181 ggttcgtggt agtttgttga taatgcactt tggtaacatt ttgctaagtt gtattgtaaa
     6241 caggttttgg ccgcaagtat tgcacatcag ggcttggtga ggtttgctct ttttgagctg
     6301 gttacaaagt tttgcgtttt cagaaaacgc aaagctttgt aaccccgcca ttttgctgcc
     6361 tacataggta aactatacaa aaacaatctt tttcgttcca aattgaagaa aatcagaaaa
     6421 aaaattacct gacaggcagg ggaaggctga ttttgaagtt tgtcccagct tcaggttcac
     6481 tatatacatt aataaaacca ttatgcaaat cgataaattc tttgcataag atgagcccca
     6541 gaccggtacc tttttcatta ttagtgccag ggatagatat tttttggtca attttaaata
     6601 agttttgcaa tgttgagggc ttcattccct tacccatatc tgacacaact atttttgtca
     6661 tttctttgtc actttcacta taaatgagta cttccgaatg agggtatgaa tatttgattg
     6721 agttgctaac tatatttcgg ataacagtct ttaaaaggtt tacatcggca aaaacaacct
     6781 cattgccggg aatacggttt agcactttga catttttact aaaagcctgt ccttcaataa
     6841 ttttaatacc ttgttccaca atttgataaa ggttgtgata gttgggttca aattttattt
     6901 ggcctgtttg cgagcgcgac cattgcaaaa ggttttcaag caattcataa gcattacggc
     6961 tcgactcgtt aataagcttg ctcatttcaa tcatttgctg tacgtcaatc tcatcagaat
     7021 tatacataat ctccgatatg cctaaaagcg cattgaaagg gtttctcagg tcgtgtgcaa
     7081 tgatagaaaa aaacttgtct tttgtggcat ttagctcaat caggctttgt tcggcattct
     7141 ttcggactgt gatgtctctg atgacggcaa caatgctatt aaggttttct tttatctcat
     7201 aatcaagcaa aacgctcctt gcttcaaacc atagcacctt tcccctgtgt tcaataggaa
     7261 attctgcaat ttgtatggaa ttgcttttta tcgtcctttc aattaaatct ctaagtatag
     7321 tatgcaaatc aacaggcagg acattgaata tattgcttga ttttgcctga agaagtattt
     7381 tgtatgcttc taatttttca ttggtaatgg catctatcaa aaaaccatct tcagcaagca
     7441 ccagaatcag ttcaggaagg gctttggtaa gcgtatcaag atattgctgg ctttttctga
     7501 gtttctcttc atattctttt tgaagcgaaa tatcaatctt tatcgccgca aaatgcgaaa
     7561 tttcgttatt atcattaata atgggagtaa taaactgttg ttcccaatac aaagagccat
     7621 cttttctgcg gtttttaaaa gcatctttcc aagttttacc agaagaaata gtatgccaca
     7681 tggccttata atattcgtct gggtggtatc cggatttgaa caccctggta tgcataccaa
     7741 tggcttcttc ggctgtgtat ccggtgattt tagtaaatat tgggtttaca tattcaataa
     7801 ctccctgctt atttgtaata acaatagatg caggactatt ttcaacaaca ctgttcaggc
     7861 tgcggttaac caacttaagg tgtgcatttt ctaagcgaag tgtttccaac tcccttctta
     7921 agtctgatat ttctttattc tcagccattt gttacttttt agcttattaa agttataaaa
     7981 aactaataag tataacaaat gatataaagg ttagttcttt gataaagaaa aaagccaggc
     8041 aaaacctggc tttaggggtg gaagatggga ctcgaaccca cgaccaatgg aaccacaatc
     8101 cactactcta accaactgag ctacatccac cattt
//
LOCUS       DAOSPF010000108         8070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8903_length_8070_cov_5.686213, whole genome shotgun sequence.
ACCESSION   DAOSPF010000108 DAOSPF010000000
VERSION     DAOSPF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_8070_cov_5.686213"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PK252_13860"
     CDS             <1..1131
                     /locus_tag="PK252_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOT15838.1"
                     /translation="GVATQEEELRKRFTGKSDYLVNYFTMLSQEIREIMAEIGITKMD
                     DLVGRADLLTSRSYPDHWKAKTVDVSKLIFMPAEAKVYPLRFMTPQHHDIEGQIDHDL
                     IAISAAAIKNKEKVWASKCITNVDRTVGAMLSGVISKQYGDEGLPEDTINVRFTGSAG
                     QSFGAFMTKGITFRLEGEANDYLGKGLCGGKIIVVPPASSTFVPEENIIVGNTLLYGA
                     TSGQVYIRGIAGGRFGVRNSGAHAVVEGAGDHCCEYMTGGRVVVLGKTGRNFAAGMSG
                     GIAYVLDVDGDFDFFCNKGLVELSPLESNDDIKEVQTMISNHLHYTNSSLAERILTNW
                     NDYAAKFVKVIPLEYKKVLEQQKLEALEAKLAMSEDEPHVRY"
     gene            1224..2648
                     /locus_tag="PK252_13865"
     CDS             1224..2648
                     /locus_tag="PK252_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOT15839.1"
                     /translation="MGNPKGFMTIQRVEAGNRPISERIEDFSEVEQTLNDADRQLQAS
                     RCMDCGIPFCQWSCPVGNTMPEFQDAVYKGNWEEAIKILHYTNNFPEFTGRICPAPCE
                     KGCVLNIHKEPVTIRENECATVEKGFALGYIKPNPAKKKTGKKVAVIGSGPAGMAAAD
                     LLNKWGHTVTLFEKDEAVGGLLRFGIPDFKLNKVVIDRRVELMIAEGLIVKTNTHVGV
                     DVKGQDIMKEYDAVCIAIGAMVPRDLPVPGRELKGIHFAMDFLTQQNRVVRGTEFSAE
                     QRINAKGKHVLVIGGGDTGSDCVGTSNRHKAKSVTQIEIMPQPPVNRTADNPWPYWPL
                     VLKTSSSHEEGCNRMWSLATKRFIGENGQVKQVEVIEVKWEKDASGRMNMVEVPGTEK
                     IIEADLVFLAMGFVSPVHEGLLNELGVEYDARGNVKASADKKTSVDKVFVAGDATRGP
                     SLVVHAIAEGRKMAESVHAFLMNK"
     gene            complement(2714..3925)
                     /locus_tag="PK252_13870"
     CDS             complement(2714..3925)
                     /locus_tag="PK252_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOT15840.1"
                     /translation="MRKLAFIILLVALASKTYSQCAASDIAIICKTADYIISHTAYTV
                     SDKEGKAYTTTQGLVPDKQYNITSPYLEWKYWNGVLNLAMFELSSVTGDEKYKQQALK
                     NYRFIFDNISFFEKLYTSGIQNTGMDQYYRMEMLDDCGAMAAGLCEAYALDKNERYLD
                     YLHKTAEYMLHKEYRLKDGTLARTGPYDKTVWLDDLYMSVPFLARYGKLNNDTSFFSF
                     AAKQIALFTHYLYDKYNGLYYHCYFDSMKQNGVAHWGRANGWCIMAQVNLLGCLPIYS
                     PLKDTLLAIYRQQVNNLARYQSESGLWHQLLDKSDSYLETSCSAMFTYAIAKGVNEGW
                     LDKRYSTIALAGWKGLKTKIQPDGQVADICIGTGIENNLVFYYKRPTMLNDIHGLGAV
                     LLAGCEVVKLK"
     gene            complement(3930..5162)
                     /locus_tag="PK252_13875"
     CDS             complement(3930..5162)
                     /locus_tag="PK252_13875"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HOT15841.1"
                     /translation="MKNILTLILIILFGSNVFFAKELQVILKNTANIERNDEMIVITR
                     DSVIKILGEISADKTLIVYDSKKSLPSQLDDLNGDGKWDELAFLADFKPQEQLTLAIK
                     IINKAESPKYGLRTNIRFAKTIKTGKIYEEAGSATRLKGFVVDSTKHYPQFEGPGWEN
                     DKVAFRNYFDERNGIDIFGKTTSKMVLDSVGIKEEYHYLKPWGMDVLKVGNSLGAGSL
                     GVCYKDSLYRISAKEGASFKIVAEGPVRSVLDLDFAKVQTGNLTFALKHRISIVAGEY
                     GYRSEVHVMHPSDSFLLATGLVNLHCKELFTAATDGCNIFYTHDKQSENNDILGLGII
                     LKKNDFIDWFQTTNNGSGVTNTFAIKMPVTSSLPVKFIFIAGWELSSPLFSDKKLFEG
                     YLKQQAVKFENPIVYQLK"
     gene            complement(5213..6001)
                     /locus_tag="PK252_13880"
     CDS             complement(5213..6001)
                     /locus_tag="PK252_13880"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOT15842.1"
                     /translation="MTNKLFDLTGKTALVTGASHGLGMEIAIGLAEAGAKVVFNNHNA
                     EKKIEAEKAYEKAGFDIKGYLFDVTKEAEVIENIQKIEKEVGPIDILVNNAAIIKRIP
                     ILDMKVEEFREVMDIDLVAAFIVSKHVVKGMIERRHGKIINICSMMSELGRDTVAAYA
                     AAKGGLKMLTKSMATEWAKYNIQVNAIGPGYFATSQTASLQATGNPFNDFIISRTPAG
                     KWGDPAQLKGPAVFLASEASDFVNGHVLYVDGGILATIGKPVNT"
     gene            complement(6003..6836)
                     /gene="kduI"
                     /locus_tag="PK252_13885"
     CDS             complement(6003..6836)
                     /gene="kduI"
                     /locus_tag="PK252_13885"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOT15843.1"
                     /translation="MKIEERYASNPDGFIAYDTERIRKDFVINKLFEKDEISLVYSHY
                     DRFIVGGIMPVKAIKLDSFGLLKSDWFLERREIGIINIGGKGKVIVDGEIFEMNFKDA
                     LYLGKETHEVTFSSLDSANPAKFYFNSALAHRKYPSRLVRFEEAEKAILGGAENSNYR
                     QLNKLLVSSILPTCQLQMGLTEIQAGSVWNTMPAHTHSRRMEVYMYFNLPENQAVCHF
                     MGRPHETRHVWLHNEQAVISPPWSIHSGVGTSNYSFVWGMAGENYDYSDMDICTINQL
                     K"
     gene            7020..>8070
                     /locus_tag="PK252_13890"
     CDS             7020..>8070
                     /locus_tag="PK252_13890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15844.1"
                     /translation="MKKNNQQSRAKTWSKQVKMYCLAGLLMVSALSSFGAARFYEVTG
                     NVSSKEGNEPVIYALVALGSQADSSIVMTTYTDSLGNYQLANVTDGDYYILAQMVGYD
                     KVKEPIMLDTTKNNVRKDFQLSIKQTKEVNVVAKRPFIESKSDRLVMNMDAQLASVGE
                     SVFENLKKAPGVYIDKDDNINLRGKQGVLVMVNEKPTYLSGNQLTNYLKGMQVSEVEK
                     IEIIINPPAKYDASGNSGIINIKLKKNSKIGLNGTMWAGAWRSEYTSGNFGGNANYRA
                     GKLNVFASVYPGEYKGYNTMDLDRYVQFNNMTTKLSQFSRGNWDYKVLGYNAGADYDL
                     SSRQTIGFMTRLGNNI"
BASE COUNT         2347 a         1630 c         1687 g         2406 t
ORIGIN      
        1 ggtgttgcta ctcaggaaga agaattgcgt aaacgcttta caggaaaatc tgattacctg
       61 gttaactatt tcactatgct ttcgcaagaa attcgtgaaa ttatggctga aattggtatc
      121 accaagatgg atgacctcgt tggacgagcc gatttactta ccagtcgcag ctatccagac
      181 cattggaagg caaaaactgt tgacgtttca aagcttattt tcatgcctgc tgaagcaaaa
      241 gtttatcctt tgcgtttcat gacccctcag caccatgata tcgaaggcca gatagatcat
      301 gacttaatag ctatatctgc tgctgccatc aagaacaagg aaaaagtgtg ggcatcaaaa
      361 tgtattacta atgttgaccg cacagtcggg gcaatgcttt cgggcgttat ttcaaagcaa
      421 tacggagacg aaggccttcc tgaagatact atcaatgtac gttttacagg ttcagcaggc
      481 caaagctttg gtgctttcat gacaaaaggt attactttcc gtcttgaagg cgaagctaac
      541 gactatcttg gcaaaggctt atgcggtggg aaaattatcg ttgtcccgcc tgcaagctca
      601 acattcgtgc ctgaagaaaa tattattgta ggaaatactt tactttatgg tgctaccagc
      661 ggacaagttt atattcgtgg tatagcgggt gggcgttttg gtgttcgtaa ctccggggca
      721 catgctgttg ttgagggtgc tggcgaccac tgttgcgagt acatgacagg tggccgtgta
      781 gtggtacttg gcaaaacagg ccgtaacttt gctgctggta tgagtggtgg tattgcttat
      841 gtgcttgatg ttgatggcga ctttgatttc ttctgcaaca aagggttggt ggaactttca
      901 ccactcgaaa gcaatgacga tatcaaggaa gtacagacca tgattagcaa tcacctgcat
      961 tataccaaca gctcgttggc cgaacgtatt ttgaccaatt ggaacgacta cgctgctaag
     1021 tttgtaaaag taattcctct tgaatacaag aaagtacttg aacaacaaaa gcttgaagct
     1081 ctcgaagcca agttggccat gagtgaagac gagccacatg taaggtatta aaattttcga
     1141 ttgatgattg ttggttgaaa aattaatcag caatggtcat tcaacaatta acaatattaa
     1201 cagacaatta ataatttaaa gatatgggaa atccaaaagg atttatgaca atacaacgag
     1261 tcgaggctgg caaccgcccg atttcggaac gtatcgaaga cttcagcgaa gttgagcaaa
     1321 cgctgaatga tgccgacaga caattacaag cttcacgttg catggactgt ggtattccat
     1381 tttgccaatg gagttgcccg gtaggcaata ccatgcccga atttcaggat gctgtttaca
     1441 aaggcaactg ggaagaagcc atcaaaattt tgcattatac caataacttc cctgaattta
     1501 cagggcgtat atgcccagcc ccttgcgaaa aaggttgtgt acttaacatt cacaaagaac
     1561 ctgtaactat tcgtgaaaac gaatgtgcta cggttgaaaa aggttttgct ttgggctata
     1621 tcaaaccaaa tcctgctaaa aagaaaacag gtaaaaaagt agctgtgata ggttcaggac
     1681 ctgcaggtat ggctgctgct gaccttctca acaaatgggg acacaccgtt accttgtttg
     1741 agaaagacga agctgtagga ggattgctcc gctttggtat ccctgacttc aaacttaaca
     1801 aggtggtaat tgacaggcgc gttgaattga tgatagccga aggccttatt gttaaaacca
     1861 atacccatgt tggtgttgat gtaaaaggcc aggatattat gaaagaatat gatgctgttt
     1921 gtattgccat aggggcaatg gttcctcgtg atttgcctgt tcctggcaga gaactcaaag
     1981 gaatacattt tgccatggac tttcttacac aacaaaaccg tgtagtacgt ggaacagaat
     2041 tctctgccga gcaacgcatc aacgctaaag ggaagcatgt acttgttatt ggcggtggtg
     2101 ataccgggag cgactgtgta ggtacatcaa accgccataa ggccaagagc gtaacacaaa
     2161 ttgaaattat gccacagcca cctgtcaaca ggactgcaga caatccttgg ccttattggc
     2221 ctttggtact aaaaacttct tcatcacacg aagaaggttg taaccgtatg tggagcctgg
     2281 ctaccaaacg tttcattgga gaaaatggcc aggtaaagca ggttgaggtt attgaggtaa
     2341 aatgggaaaa ggatgcttcg ggcagaatga acatggtaga agttccagga actgaaaaga
     2401 ttattgaagc agacttagtg ttccttgcca tgggctttgt ttctccagtt cacgagggct
     2461 tgcttaacga gcttggcgta gagtatgatg cccgcggtaa cgtaaaagcc tctgctgaca
     2521 agaaaaccag cgtggacaaa gtatttgttg ctggagatgc tacccgtggc ccatcacttg
     2581 tagtacatgc tattgccgaa ggccgtaaaa tggcagaatc tgttcatgct ttcctgatga
     2641 acaagtaaaa tattcacttt tcataggtaa aacgctttgt atctttttga tgcaaagcgt
     2701 tttcatttta aagttatttc aatttcacaa cttcgcaacc ggccagtaat acagctccca
     2761 gaccatgaat gtcgttgagc atggttgggc gtttgtaata aaacaccagg ttgttttcaa
     2821 tgcccgtgcc aatgcagatg tcagcaactt gtccgtcagg ttgtattttt gtttttaatc
     2881 ctttccatcc ggcaagggca attgtgctat agcgtttgtc gagccagcct tcattaacac
     2941 ctttagcgat ggcataggta aacattgccg agcaggaagt ttcgaggtaa gagtcgcttt
     3001 tgtccagcag ttgatgccaa agcccgcttt cgctctgata ccttgccagg ttgtttacct
     3061 gttggcggta tatggccaat agcgtgtctt tcaatggtga ataaatcggt aagcagccca
     3121 aaaggttaac ctgggccata atacaccatc cgttagccct tccccaatga gctacgccgt
     3181 tttgcttcat actgtcaaaa tagcaatggt aatacagccc gttatatttg tcgtacaaat
     3241 aatgcgtaaa cagggctatc tgttttgcag caaagctaaa aaacgatgta tcgttgttta
     3301 gttttccgta acgtgccaga aagggaacgc tcatatacag gtcatcgagc catacagtct
     3361 tatcatatgg gccagtgcgg gcaagggtac catctttcaa ccgatattct ttgtgtagca
     3421 tatattcggc agttttatgc aggtaatcga ggtacctttc atttttgtcc aatgcatagg
     3481 cttcgcaaag tcctgctgcc attgcaccac agtcatccag catctccatt cggtaatact
     3541 ggtccatccc tgtattttgt atcccagatg tgtaaagctt ttcaaagaaa ctgatattat
     3601 caaagataaa acggtagttt ttcaaagcct gctgtttata cttctcatcc ccagtaacac
     3661 tagacaattc gaacatagca aggttcaaca caccattcca gtacttccat tcgagatacg
     3721 gactggtgat attatactgt ttatcaggca caagaccttg tgtagttgta taggcctttc
     3781 cttctttgtc tgaaacagta taagcagtgt gagagataat ataatctgca gttttgcaaa
     3841 tgatagctat atctgatgct gcacactggc tataagtttt ggaggctaat gctactaaaa
     3901 gtattatgaa agctagtttt ctcattttct tattttaact ggtaaacaat tggattttcg
     3961 aatttcacag cttgttgttt taagtaacct tcaaatagct ttttatcaga aaataaaggg
     4021 cttgataatt cccatccggc tataaaaatg aatttaactg gcaaggatga ggtaactggc
     4081 atttttattg caaaagtatt ggtgactcca ctaccattat ttgttgtttg aaaccaatct
     4141 ataaagtcat tctttttaag aataatccct aatcccaaga tgtcattgtt ttctgattgt
     4201 ttatcatgcg tgtagaagat attgcaacca tctgtggcgg cagtaaacaa ttctttgcaa
     4261 tgtaggttaa caagtccagt agcaagcaag aagctatctg aaggatgcat cacatgtact
     4321 tcacttcgat acccatattc tccagcaaca attgatatcc ggtgtttcaa ggcaaaggtg
     4381 agattgccag tttgtacctt agcaaaatca agatctaaca ctgatcttac cgggccttca
     4441 gcaacaattt taaagcttgc accttctttg gcgctgatac gatagagact gtctttgtaa
     4501 caaacgccta acgagcctgc accaagagaa ttacctactt tcagaacatc cattccccaa
     4561 ggcttcaggt agtgatattc ttcctttatt ccaacgctgt cgagcaccat ttttgaggtg
     4621 gttttgccaa agatatcaat gccatttcgc tcgtcaaagt aattacggaa tgccaccttg
     4681 tcgttttccc aaccagggcc ttcgaattga gggtaatgtt ttgtactatc caccacaaaa
     4741 cctttcagac gagttgccga acctgcttct tcatatattt tgccagtttt tatagtcttt
     4801 gcaaaacgaa tgttggtacg gagcccatat tttggagatt ctgctttgtt gattatttta
     4861 atagccaatg ttaattgttc ctgtggtttg aaatctgcca gaaatgccag ttcgtcccat
     4921 ttcccatcac cgtttaggtc atcgagctgt gaaggaaggc tttttttgct atcataaact
     4981 atcaatgttt tgtcggcgct gatttctcca agtattttaa taacagaatc tcgagtaatg
     5041 acaatcatct cgtcatttct ttcaatattg gcagtatttt taagaattac ctgtaattcc
     5101 tttgcaaaaa agacattgga accgaaaaga ataatgagta taagagttaa gatgtttttc
     5161 ataatgttat tttttagaac caagaaacaa aagataatct tgactcttgg ttttacgtat
     5221 ttacaggttt cccaatggta gcaagtatgc cgccatcaac ataaagtaca tggccattaa
     5281 caaaatcaga tgcttcagaa gcaaggaata ctgccgggcc ttttagttgt gccggatcgc
     5341 cccacttccc agcaggtgta cggctaataa taaagtcgtt gaacgggttg cccgtggctt
     5401 gcaaagaagc agtctgcgat gtagcaaaat agcctgggcc tatggcattg acctgtatat
     5461 tatactttgc ccattcggtg gccatactct tggttagcat tttcaaacct cctttggcag
     5521 cagcataagc agcaacagta tcgcgtccca gttcgctcat catagaacat atgttgataa
     5581 ttttgccatg tctcctttca atcattcctt ttaccacatg ttttgatact ataaatgctg
     5641 caaccaaatc aatatccatg acttcacgga attcttctac tttcatatcc aatatgggaa
     5701 tgcgtttgat gatggcagca ttgtttacca aaatatctat cggaccaact tctttctcaa
     5761 ttttctggat attttcaatc acttctgctt ctttggtaac atcgaaaagg tagcctttga
     5821 tatcaaatcc agccttttca taagctttct ctgcttctat ttttttctcg gcattgtgat
     5881 tattgaatac gaccttggct ccagcttcgg caagcccaat ggctatttcc atccctaagc
     5941 catgtgaggc acctgttaca agggcagttt tcccggtaag gtcaaaaagt ttatttgtca
     6001 tattacttta gctgattgat agtacatata tccatgtcac tgtaatcgta gttttcaccg
     6061 gccatacccc atacaaagct gtagtttgag gtacctactc cactgtgtat tgaccacgga
     6121 ggcgaaatga cggcttgttc attgtgtaac catacgtgcc ttgtttcatg gggtcgtccc
     6181 ataaagtggc aaacagcctg attttcaggc agattgaaat acatatagac ctccattctc
     6241 ctgctatggg tatgggctgg catagtattc catacgctac cggcttgtat ctcggtcaac
     6301 cccatttgta gctgacaggt aggcaatatc gaactaacca gcaacttgtt caattgcctg
     6361 taattggagt tttcagcccc acctaaaata gctttttcgg cttcttcaaa acgtaccagt
     6421 ctagatggat attttcgatg agccagtgca gaattgaaat aaaattttgc cggatttgca
     6481 ctatctaaag atgaaaaagt aacttcatgt gtttcttttc ccaggtataa tgcatctttg
     6541 aagttcattt caaagatttc cccatcgaca ataactttcc ccttccctcc aatattgatg
     6601 atgcctattt cacggcgttc gagaaaccag tcagacttta acaagccaaa agaatcaagc
     6661 ttgattgcct ttaccggcat tatccctcca acgatgaatc tgtcgtaatg cgaataaaca
     6721 agggatattt cgtctttttc aaaaagctta ttgatgacaa aatctttccg gatgcgttct
     6781 gtgtcataag ctataaaccc atcagggttg ctggcatagc gttcctcaat tttcattggt
     6841 aatatttttt acaaaagtat aatttttata atcagttgta aagtatttat ttcagaaaaa
     6901 ctcccttttt acaatttttt acaattggtt gcacctcttt acaacatgtt actattggta
     6961 tacaaatgcc aaggtagttt tgtatcataa aaataacaat ttacttatcg tacaaaacaa
     7021 tgaaaaaaaa taaccagcaa tcaagagcaa aaacctggtc taaacaagtc aaaatgtatt
     7081 gtttagccgg tttattgatg gtgtcagctt taagcagttt cggagctgcc cgtttttatg
     7141 aagttactgg caatgtcagt agcaaagaag gcaatgagcc agtaatttat gcattagtag
     7201 ctttggggtc tcaggccgat agcagtattg tgatgacaac atatactgac agtttaggta
     7261 attaccagtt agctaacgtc acggacggag attattacat tcttgcccaa atggtaggtt
     7321 atgacaaagt taaagaacct atcatgctgg atactacaaa gaataacgtc cgaaaggatt
     7381 ttcaactttc tataaaacaa actaaagagg tgaatgtagt ggccaaaagg ccgtttattg
     7441 agtcaaaatc cgaccgtttg gtaatgaata tggatgcaca gcttgcttct gttggcgaat
     7501 cagtttttga aaacctgaaa aaagctcccg gggtttatat tgacaaagat gacaacatca
     7561 accttcgtgg taaacagggc gtgttggtta tggtaaacga aaaacccacc tatctgtctg
     7621 gcaaccagtt aaccaattac ttaaaaggca tgcaggtgtc agaggtggag aaaattgaga
     7681 ttatcatcaa ccctcctgca aaatatgatg cctcaggtaa ttcgggcatt attaacatta
     7741 agttgaaaaa gaacagcaaa attgggttga atggtaccat gtgggcagga gcctggcgta
     7801 gcgaatatac cagcggcaac tttggcggca atgcgaacta tcgcgcaggg aagctcaatg
     7861 tttttgccag tgtttatccg ggcgagtata aaggttacaa cactatggat ttggacagat
     7921 atgtgcaatt caacaatatg actacaaaac tttcacagtt tagccgtggc aactgggatt
     7981 ataaagtgct aggatataat gctggtgcag attatgacct gagcagtcgc caaacaattg
     8041 gttttatgac ccgtttgggg aacaacatag
//
LOCUS       DAOSPF010000109         8033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8958_length_8033_cov_7.961770, whole genome shotgun sequence.
ACCESSION   DAOSPF010000109 DAOSPF010000000
VERSION     DAOSPF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8958_length_8033_cov_7.961770"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PK252_13895"
     CDS             <1..455
                     /locus_tag="PK252_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT15845.1"
                     /translation="DTVKRAIRAIEIAEYQKNHISNSPNRPVINSLIIGIDFEREIVR
                     KRITERLHQRLEEGMIDEVKSLLNKGLHSEDLIYYGLEYKYITLYLIGQLTYDDMVAQ
                     LNIAIHQFSKRQMTWFRKMERSGFKIHWVEGEMPLGEKLNSVFSLLNF"
     gene            complement(467..1873)
                     /locus_tag="PK252_13900"
     CDS             complement(467..1873)
                     /locus_tag="PK252_13900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT15846.1"
                     /translation="MTKSKFGFLKDIRFWIFIFFIIRLYGITDAPLEHSHNWRQCTGL
                     MVSRNFLEIDNNILYPRVDDNYGYSGIVGMEFPIMNYIVYLISKVFGFTWWYGRILNL
                     IITSIGIFYFFKIIEKFFDKKIAFYSTLVLLSSIWFVFARKFMPDTFSLSITIISLWY
                     GFLFLEKGKLIHWGIFFILTFLGIMSKIPAIFLLSLYIIPIVKEKKINLRRKIIFSFS
                     FIPTILAVWFWYFYWNPHLSNEYHLWYNSGRSLSEGFYELTNNLNKVAERFYFDGLKS
                     YIGLLTIFLGIFFMIKLLDKLAWAAILILTSVFFIYMIKSGYFFYHHDYYIIPYVPVM
                     ALIAGYGISKINAKLRFFLIALVIIESLLNQQHDLTNKLETYRLNLEVISDSISSKSD
                     LIAVNGKGNPQELFLSHRKGWTLSNENIIDTVTLRNIKEKGCKFIYINKHECNDKLPK
                     GFNKVFENEDYIIFSINQ"
     gene            complement(1883..2284)
                     /locus_tag="PK252_13905"
     CDS             complement(1883..2284)
                     /locus_tag="PK252_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15847.1"
                     /translation="MAFVKFIFIAAFIILFFVTVIMLKPLSKHRKYPYTTFALKFSFL
                     FYMVFALAMTYFFLFDYASTVAFFEDIENPRASFHFTILLFTLFIPAVGILLRRKFTK
                     RKVYNLIFTGINFSCILYYVFLAKIVFRIAF"
     gene            complement(2298..4370)
                     /locus_tag="PK252_13910"
     CDS             complement(2298..4370)
                     /locus_tag="PK252_13910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT15848.1"
                     /translation="MKKLLIGLSVTILVSFSCKKNDVQVFFETAITDSEKIVGKLTPE
                     MLWKFGRILDWDISPDGKNIVYTVKRYNLQKNNGNTEVFIIPSQGGTPARLTITAESE
                     SCVKFSAKGDKIGYLSSLSGSSQIWEMNPDGSNKTQVTYVKGDIEGFDYSPDGSKVFF
                     TMRVKTDSSAQDRNPDLPLSTGRVYDDMMYRHWDSWYDYSHSHIFIAPIKDGKVTDAE
                     DIMKGEPYDAPLRPYYDLNEISWSNDGKKLAYTCRKLKGKAEALSTNSDIYIYDVTDK
                     TTVNISEGMNGYDKYPVFSPDDKMICWTSMETPGYESDQTRLMVYNFETKQLKNLSAN
                     FEQEAISPVWDEQSNAIYFISGINATYQLYKVDLKSNSIKQLTQGGHEYIALLRAAGT
                     MIGTRMSFSRATEIINLSDEGKEQQVTFENQNIYDNIRMGKVEERWIATTDGKKMLTW
                     IVFPPDFDSTKRYPALLYCQGGPQQAVSQFFSYRWSLQLMAARGYIVVAPNRRGLPTF
                     GKAWNDQIAGDYGGQNIKDYLSAIDAIKTEPFVDSEHLGAVGASYGGFSVFYLAGHHQ
                     KRFKAFIAHCGMFNLESQYASTEEFFFTNHDLGGPYWQTPKPKSYSFSPHLFVQNWDT
                     PIMIISGENDFRIPYTESLQAFNAAQLRSIPSRLLIYPDESHWVVKPQNSVLWQREFF
                     RWLDKWLK"
     gene            4596..5051
                     /gene="rplM"
                     /locus_tag="PK252_13915"
     CDS             4596..5051
                     /gene="rplM"
                     /locus_tag="PK252_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT15849.1"
                     /translation="MNTLSYKTISVNKATAQKEWVIVDAQNQALGRLSSKVAKLLRGK
                     YKPSYTPHVDCGDNVIVINADKVRITGKKWTDRKHFSHTGYQGGQVVLSPLDIFKKNP
                     CRLIEKSVKGMLPKNILSNKLIGNLYVYVGENHKHEGQQPKSIDINSLK"
     gene            5057..5443
                     /gene="rpsI"
                     /locus_tag="PK252_13920"
     CDS             5057..5443
                     /gene="rpsI"
                     /locus_tag="PK252_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT15850.1"
                     /translation="MEIINALGRRKTAVARVYLKEGKGNITINDRALSTYFTTTQLQY
                     IVKQPLEKLGVAENFDVTVNITGGGFKGQAEAVRLGIARALVKINEENKPALKKEGFM
                     TRDSREVERKKPGRPKARKRFQFSKR"
     gene            5551..6414
                     /gene="rpsB"
                     /locus_tag="PK252_13925"
     CDS             5551..6414
                     /gene="rpsB"
                     /locus_tag="PK252_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT15851.1"
                     /translation="MSKVTFEQLLDAGVHFGHLKRKWCPAMAPYIFMEKNGIHIIDLQ
                     KTAAKLEESANAIKQIAKSGRRILFVATKKQAKEIVSEKVKAVGMPYVTERWPGGMLT
                     NFPTIRKAVKKMTSIDKMIADGTFDTLSKREKLQITRQRAKLEKNLGSIADLTRLPAA
                     LFVIDITKEHIAVHEAKRLGIPVFAMCDTNSDPNKVDFAIPANDDASKSIALIVDVIC
                     DAIKEGLSERKVDKDAEQAERKAKQDVKKKVTKKSIDELEEVLPEAVKVVAETETKKA
                     PIKKKIRKDEE"
     gene            6447..7274
                     /gene="tsf"
                     /locus_tag="PK252_13930"
     CDS             6447..7274
                     /gene="tsf"
                     /locus_tag="PK252_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT15852.1"
                     /translation="MSQVSLADITKLRTATSAGMMDCKKALEEAGGDFEKAVEIIRKK
                     GQLVASKRAERTATEGVVLAAITADGKFGAVTALNCETDFVAKNADFVALAQKISDAA
                     IANKPKDADALKAIIVDGRSIADLITERIGVTGEKMDLSYYGFIQAEKVVAYNHPGNQ
                     LACVVGFNKDADAQVLKDVAMQVAAMNPVAVDKDSVSKEKIEKEMEIGREKARLEGKP
                     ENMIEKIAEGMLNKFYKESTLLNQEFIKDNKKTIRQYIQGVDKDLNVVGFSRCGLKD"
     gene            complement(7461..>8033)
                     /locus_tag="PK252_13935"
     CDS             complement(7461..>8033)
                     /locus_tag="PK252_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOT15853.1"
                     /translation="KAMKGVKFDYNAKMYNNDKSLQSKLGKNKVGFIAQDLQKVLPQA
                     VSYDDSTGIYAVDYTKVIPVLVEAIKEQQKVIDQLVGKSNEKASSKKESANTDIASAN
                     AVIASLGQNAPNPFNQSTVIVYYLPETVQNAMLNVYDMNGLQIKTFGHLQTGKGSITV
                     HAAELRPGMYLYSLIADGKEIDTKRMILTE"
BASE COUNT         2602 a         1456 c         1517 g         2458 t
ORIGIN      
        1 tagatacggt aaaacgtgct atccgagcta ttgagattgc agaataccag aaaaatcata
       61 ttagcaatag ccctaacagg ccagttatca atagtcttat tattggtatt gattttgagc
      121 gggaaatcgt ccgaaaacgt ataactgaaa ggctgcatca aagacttgaa gaaggaatga
      181 ttgatgaagt aaagagtttg ttaaacaaag gtctgcattc tgaggatttg atatactatg
      241 gattggaata taaatatata acgctctatt taattggtca attgacttat gatgacatgg
      301 tagcccagct caatattgct attcatcagt tttcaaaacg ccaaatgacc tggttccgca
      361 agatggagcg cagtgggttt aaaattcact gggttgaagg ggagatgcct ttaggagaaa
      421 aattaaattc agtttttagt ttattgaatt tttaactttt tgcagattat tgatttatag
      481 aaaagattat ataatcttca ttttcaaata ctttattaaa tccctttggt aatttatcat
      541 tgcattcatg cttgtttata taaataaact tacacccttt ttcttttata tttctcaggg
      601 taactgtgtc tataatgttt tcatttgata atgtccatcc ttttctatgt gaaagaaaaa
      661 gttcttgtgg gtttcctttt ccgtttacag ctattaaatc tgacttagat gatatactat
      721 cagaaataac ttctaggttt aaacgataag tttcaagttt atttgttaaa tcatgctgtt
      781 ggttaagtaa cgattcaata ataactaagg ctataagaaa aaaacgaagt tttgcattta
      841 tttttgaaat tccataacct gctatcagag ccattaccgg aacatatggg atgatataat
      901 aatcatgatg ataaaagaag tacccagatt taatcatata aataaaaaac actgaggtaa
      961 gaatcaaaat agcagcccaa gctaacttat ctaatagctt aatcataaag aaaattccca
     1021 gaaatattgt taatagtcca atataacttt ttaggccgtc aaaataaaat ctttctgcaa
     1081 ccttatttag gttgtttgtt aattcataaa aaccttctga taagcttctt cctgagttgt
     1141 accacagatg atattcattg cttaaatggg gattccaata aaagtaccag aaccaaacag
     1201 ctaatattgt tggaataaag gagaatgaaa aaatgatttt tcttcttaaa ttaattttct
     1261 tctcttttac aattgggata atatatagag aaagtaaaaa tattgccggg attttactca
     1321 ttatccctaa aaaagtgaga atgaaaaata taccccaatg aataagttta cctttttcta
     1381 aaaataaaaa tccataccaa aggctaataa ttgttatgga caaagaaaag gtgtccggca
     1441 taaattttct ggcaaaaaca aaccaaattg aagagagtaa tactaatgtt gaataaaatg
     1501 ctattttttt atcgaagaac ttttctatta ttttaaaaaa ataaaatatt cctatggaag
     1561 tgataatcaa gtttaaaatc cgaccatacc accaggtaaa cccaaaaact tttgaaatca
     1621 aataaactat gtagttcatt attggaaatt ccattcccac tatacctgaa taaccataat
     1681 tatcatcaac tcttggatat agaatattat tgtcaatttc taaaaaattt cttgatacca
     1741 ttaatccagt acattgtctc caattatggc tatgttctaa tggagcatct gtaatgccat
     1801 ataagcgaat gataaaaaaa ataaaaatcc agaaccgtat gtcttttaaa aaaccaaatt
     1861 ttgattttgt catattttaa agttaaaaag ctatcctaaa cacaatcttg gccaaaaata
     1921 cataatacaa tatacaactg aaatttattc ctgtgaaaat taggttgtac acctttcgct
     1981 ttgtaaattt cctacgtaac aagataccta cagctggtat aaacaaagta aacagcagaa
     2041 tagtgaaatg aaatgaagcc cttgggtttt ctatatcttc aaaaaaggca acagtgctgg
     2101 catagtcgaa tagaaagaaa taggtcatag ccagtgcaaa taccatgtaa aataagaaag
     2161 agaactttag tgcgaaggtt gtataaggat atttgcgatg tttgcttaat ggtttaagca
     2221 ttatcactgt tacgaaaaac aagatgataa acgctgcgat gaatataaat tttacaaatg
     2281 ccatttttgc tcttttttta ttttaaccat ttgtctaacc acctgaaaaa ctctcgttgc
     2341 cagagtacac tattttgagg cttaactacc caatggcttt catcggggta tatcaacaac
     2401 cttgaaggta tgctccgaag ttgagctgca ttaaatgctt gaaggctctc tgtataaggg
     2461 atcctaaaat cgttttcgcc agaaataatc ataattggcg tgtcccagtt ttgcacaaac
     2521 aagtgaggcg agaatgaata gcttttaggc ttaggtgttt gccagtaagg cccgccaagg
     2581 tcgtgattgg taaagaaaaa ctcttcggta gaggcgtatt ggctttcgag gttaaacata
     2641 ccacaatggg caataaatgc tttgaaccgc ttttggtgat gtccggccag ataaaaaacc
     2701 gaaaagcctc catagcttgc cccaactgcc cccaaatgtt cgctatcaac aaaaggctcg
     2761 gtttttatgg catcaatagc actcaaatag tcttttatat tttgtcctcc gtagtctccg
     2821 gcaatctggt cattccaggc tttaccaaat gtgggcagcc cgcgacggtt aggagcaaca
     2881 acaatatatc ccctggctgc catcaattgc aagctccaac ggtacgagaa aaactggctt
     2941 acagcttgtt gaggtcctcc ctggcaatat aacagggcgg ggtaacgttt ggtcgaatca
     3001 aagtccggag gaaaaacaat ccacgtaagc atctttttgc catcagtggt agcaatccag
     3061 cgttcttcca cttttcccat gcgtatattg tcatatatat tttggttctc aaaagtaacc
     3121 tgttgttctt tcccttcatc cgaaaggttt attatttcag tagcacggct gaaagacata
     3181 cgtgtgccta tcatggtacc agctgcgcgg agcaatgcta tgtactcgtg accgccctgg
     3241 gtaagttgtt ttatcgaatt gcttttcaaa tctaccttgt agagttggta tgtggcattt
     3301 atcccgctaa taaagtaaat ggcattgctt tgttcgtccc ataccgggct gatagcctcc
     3361 tgttcaaagt tggccgaaag gtttttgagc tgttttgttt caaagttata caccatgagc
     3421 cttgtctggt ctgactcgta gccgggagtt tccatacttg tccagcatat catcttgtcg
     3481 tcaggcgaga acacgggata tttgtcgtat ccattcatgc cttcagaaat attgacagtt
     3541 gttttatctg ttacatcata tatgtaaatg tctgaatttg tgcttaacgc ttcagctttg
     3601 cctttcagct tgcgacaagt gtaagccagc tttttgccat cgttggacca cgagatctcg
     3661 tttaagtcat agtatggccg aagaggggca tcgtaaggct cgcctttcat tatgtcttca
     3721 gcatctgtaa ctttgccatc cttaataggt gcaataaata tatgactgtg cgagtaatcg
     3781 taccacgaat cccaatgcct gtacatcata tcgtcataca cacgacctgt ggacaagggt
     3841 aaatcagggt tacggtcttg tgccgagctg tcagtcttta cccgcatggt gaaaaatact
     3901 ttacttccat ccggcgaata gtcaaaacct tcgatatcgc cttttacata agtaacctga
     3961 gttttattgc taccatcggg gttcatttcc caaatctgcg aacttccgct gaggctgctt
     4021 aaatagccaa ttttatcgcc tttggcagaa aacttcacac aagactctga ttcggcagta
     4081 attgtaagcc ttgcaggtgt acctccttgt gatgggataa taaaaacctc ggtattgcca
     4141 ttatttttct gcaggttgta acgttttacc gtgtaaacaa tgtttttccc atcaggcgaa
     4201 atgtcccagt ccaaaatgcg gccaaatttc caaagcattt caggggtcag ttttccaact
     4261 atcttctcgc tatctgtgat agccgtttca aaaaagactt gtacatcatt ctttttgcaa
     4321 gaaaagctta caagaatggt tacggataat ccaataagga gttttttcat tgttttcttt
     4381 aataaagagt aaagttaaaa ataaagtttt tcgttgaaac ttctgtaaaa atttttcagc
     4441 tcattttatt cccagtgagt aagaaagaat atttagttaa atttggaaat attttttcgg
     4501 gctaatattt gttaataaaa aaatagttgt atttttgcag tccgcttttt gagaagaaaa
     4561 gtttgtatgt atataataat taaaaactta gcaaagtgaa tacgcttagt tataaaacaa
     4621 tttcagtaaa caaagccacc gcacaaaaag agtgggtgat tgtagatgct cagaatcagg
     4681 cgcttggacg tttgtcttca aaagttgcta aattgctcag agggaaatac aaaccaagtt
     4741 atacccctca tgttgattgt ggtgacaatg ttatcgttat caatgccgat aaagtgcgca
     4801 ttaccggtaa aaaatggaca gacagaaagc atttttcaca taccggatac caaggtggac
     4861 aagttgtatt gtctccactg gatatcttca agaaaaatcc ttgccgttta attgagaaat
     4921 cagtaaaagg catgttacca aagaatatcc tttctaacaa gctcattggc aacctttatg
     4981 tttatgttgg agaaaaccac aaacacgaag gacaacagcc aaaatctatt gacatcaatt
     5041 ctcttaaata atatccatgg aaattattaa cgcattaggc agaagaaaaa cagctgtagc
     5101 ccgtgtttat cttaaagaag gaaaaggtaa tatcactatt aacgaccgtg ctttaagtac
     5161 atattttacc acaacacagt tgcaatatat tgttaaacag ccacttgaaa aattaggggt
     5221 agccgaaaat tttgatgtta cagtaaacat tactggtggt gggtttaagg gccaggcaga
     5281 agcagttcgc cttggtattg ccagagcatt ggtaaagatt aacgaagaaa ataagcctgc
     5341 tttgaagaaa gaaggtttca tgacccgtga ctcacgtgaa gttgagcgta aaaaaccggg
     5401 tcgtccaaaa gcacgtaaac gcttccagtt cagtaaacgt taaaagtttt tgctgggact
     5461 tagtttagca tctaaattac ccggacttcc gctttggtgg ggctaccggg taattgctta
     5521 atataagaac gtaaactaaa ttttttattc atgtcaaaag taacatttga acaacttctt
     5581 gatgccggag tacatttcgg ccacttaaaa aggaaatggt gcccggccat ggctccatac
     5641 atttttatgg agaaaaacgg gatccacatc atcgatctcc aaaaaacagc agcaaaactg
     5701 gaagaatctg ccaatgcaat taaacaaatt gcaaaatcag gccgtcgtat tttgtttgtt
     5761 gccacaaaaa aacaagccaa agaaatcgtt agtgaaaaag taaaggctgt tgggatgcct
     5821 tacgttactg aacgttggcc gggtggtatg cttaccaact ttccaacaat ccgtaaagct
     5881 gttaaaaaga tgacctctat cgacaaaatg attgccgatg gcacctttga taccctttca
     5941 aaacgtgaaa aattacaaat tacacgtcaa cgtgcaaaac tggaaaagaa cttaggtagt
     6001 attgccgatc ttacccgttt gccagcagct ctttttgtaa ttgacattac aaaagaacat
     6061 attgccgttc acgaagcaaa acgtcttggt attccagtgt ttgcaatgtg cgataccaac
     6121 tctgacccca ataaagttga ctttgctatt ccggcaaatg atgatgcctc taaatctata
     6181 gctttgattg tagatgttat ttgcgatgct atcaaagaag gattgtcaga acggaaagtt
     6241 gataaagatg ctgaacaagc tgagcgtaaa gctaaacaag acgttaagaa aaaagtaaca
     6301 aaaaaatcta ttgacgagct tgaggaagtt ttgccggaag cggtaaaagt tgtagctgaa
     6361 actgaaacca aaaaagcacc tatcaaaaag aaaattcgca aagacgaaga ataatttaca
     6421 aaatataaat tgaaaatatt acagatatgt cacaagtttc tcttgccgat ataacaaaac
     6481 tcagaacagc taccagtgct ggtatgatgg actgtaaaaa agcccttgaa gaagccggtg
     6541 gtgattttga aaaagctgtt gaaatcattc gtaaaaaagg ccagttagta gcaagcaaac
     6601 gagctgaacg tactgctact gaaggtgttg ttttggctgc tattactgcc gatggcaaat
     6661 ttggtgctgt tactgccctt aactgcgaaa cagatttcgt tgccaaaaat gccgattttg
     6721 ttgctttggc acaaaaaatt agcgatgcag ctatcgccaa caaacctaaa gatgctgatg
     6781 ctttgaaagc tattattgtt gacggccgtt cgatagcaga ccttatcacc gagcgtattg
     6841 gtgttactgg tgagaaaatg gatttaagct attatggctt tatccaggct gaaaaagttg
     6901 ttgcttacaa tcatccgggt aaccaattgg cttgtgttgt tgggtttaac aaagatgctg
     6961 atgctcaggt tttgaaagat gttgccatgc aggttgctgc tatgaaccct gttgctgttg
     7021 ataaagactc tgtttcaaaa gaaaaaattg aaaaagagat ggaaatcggt cgcgaaaaag
     7081 cacgtcttga aggcaaacct gaaaatatga ttgagaaaat tgctgaaggt atgcttaaca
     7141 aattctacaa agaaagcact ttgttgaacc aagaatttat aaaagataac aaaaagacaa
     7201 tccgtcaata catccagggt gtagataaag atttgaatgt tgttggtttt agcagatgcg
     7261 gtcttaaaga ctaattggct ataccggaca ttcatatatt ttacggttaa ttatttcaga
     7321 aaagtccgcc tctatggcgg acttttttat tattaggcaa gaaaaaaacc ttccaggttt
     7381 tgaaaacctg gaaggtagag gtactggtat aaaaaacgct ttgcttttcg gaaaagcaaa
     7441 gcgtttcaac actattcaaa ttactcagtc aatatcatcc ttttggtgtc tatttcctta
     7501 ccatcggcaa taagagaata taagtacatg ccaggcctta actcggctgc atggactgta
     7561 atacttcctt tgcctgtttg caaatggcca aaggtcttta tttgcaaacc attcatgtcg
     7621 taaacattta acatggcatt ttgcactgtt tcaggtaaat agtaaacaat cacggtggac
     7681 tggttaaacg ggtttggggc gttttgcccc agcgaagcta taactgcgtt tgcagatgca
     7741 atatcagtat ttgcactttc ctttttactt gaggcttttt catttgattt gcccaccagc
     7801 tggtcgataa ccttctgttg ctctttgata gcctccacca gcacagggat aactttggta
     7861 taatcaacag cataaatacc tgtagagtcg tcataactaa cagcctgtgg caataccttt
     7921 tgaaggtctt gtgctataaa gcctactttg tttttgccca gtttgctttg caggcttttg
     7981 tcattattgt acatctttgc attataatca aactttaccc ctttcatggc ttt
//
LOCUS       DAOSPF010000110         8024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_8972_length_8024_cov_7.643619, whole genome shotgun sequence.
ACCESSION   DAOSPF010000110 DAOSPF010000000
VERSION     DAOSPF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_8024_cov_7.643619"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..951)
                     /locus_tag="PK252_13940"
     CDS             complement(109..951)
                     /locus_tag="PK252_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15854.1"
                     /translation="MKHIFLAILFYFSLYEDLTCQELMRNGSFEIVDESPNAYRFLAK
                     NWYIQNGTSVDYFSKRISSQSKIYGVPMNFLGYHPSIDGDAYCGLVLFSWNCCDYELL
                     TGSLMKSLQKDSIYFISFMLKYAGDSIILNSKEIYIYFSNDSSNLVMSNCYSGNYCDL
                     IDNIGIAINIDSVNRSNDWVNCSILYKAKGFERFVTIGLITKNNETLKKKNKEFRKLI
                     ESKASLEKIKKFVESNTLYPIGLNEKCIINDRKKYLYRTYSYYFLDKFSIMPYIESNY
                     TIEK"
     gene            complement(963..2318)
                     /locus_tag="PK252_13945"
     CDS             complement(963..2318)
                     /locus_tag="PK252_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT15855.1"
                     /translation="MAIYTQSTVTANTKLVYCLRDHLGSLQYVVNEAGTTVLEEYSYD
                     AWGCRRDPVTWKPAAITANTARGFTGHEHIDLFELVNMDGRVYDPRLGRFMSPDPYVQ
                     EPFNSQSYNRYSYCINNPLKYTDPSGYSWFSRAWSWTKSNLRSIATVAATVVVVAGVT
                     IATAGMGTVACAFLAGAAGGFTGGFAGTLLNKGSLSDAIFNGVKGACIGAATGLATAG
                     VLGAVSYGLNNLYLNTWHGAGTVTDVSWSITTKAMFEGAGSRTLASLLPSFGSALGEG
                     GGIGLLSGLSGGGVGSAIGISNNGWTKTPAGYRPTPGEQQMYYLDRQGKSIPSFRAEN
                     LRKNQSSYFEKSFVKQMTRNKGGAYTIAGNIEMPKDAQNTIAHFKGQLFDNSYAFISS
                     EGQDLYFGEFFGNLTFPAQVSIPGKGLYYHSSGNLDPNQFRNNEGLNGGLGFYLTFYK
                     N"
     gene            complement(3096..3932)
                     /locus_tag="PK252_13950"
     CDS             complement(3096..3932)
                     /locus_tag="PK252_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15856.1"
                     /translation="MHKTIFIIALLTTKLATAQENLVLNASFEEYTDLPKTSIADGIS
                     FAKNWFVPDESSPDYFHVNAPFYFLQVPNTELGFHPAHTGDAYIGLYPLNYDGYMEHY
                     TGTLKKELKKGKVYHVSFFIRYGGASCRFATKSIGVYFSKTKNPFKTWSPPYYYEILE
                     PKITASVQSNKDSILLNDTAWVEITGNYKANGGEKYITLGKFYDDEITGRLLKKYTEV
                     QFNEREKEKYYKKHTDIFPINPHYKKNPLFPVEAAYYFIDDVSVTSESDVELDFTYPI
                     NK"
     gene            complement(3941..5290)
                     /locus_tag="PK252_13955"
     CDS             complement(3941..5290)
                     /locus_tag="PK252_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT15857.1"
                     /translation="MAIYTQSTVTANTKLVYCLRDHLGSLQYVVNEAGTTVLEEYSYD
                     AWGCRRDPVTWKPAAITANTARGFTGHEHIDLFELVNMDGRVYDPRLGRFMSPDPYVQ
                     APFNSQSYNRYSYCLNNPLSFTDPSGYSWFSRACSWVGKNWKPIVTAVAAIGAGVATA
                     LTIGAASPLLVATLSGAAAGFTGGVVGTALNGGSVGQCLGAGIVGGLIGAAAGLAGGA
                     AGLGASLALNSATGISGGVVGGAFSGAVGGAVGGFVGGFGSALANRASPSQALNTGWN
                     MAAIGAVTGGILGGSIGGYKAYRAGNNIWTNQARIPVLVKSDGSYIARDVKNAYAKAE
                     PSYGKTRDFLKITNATSPGDELNITIPEKVTVTDIEVNNNGFCGDNCLSISVDNRTPV
                     LFCDAPKATWTTIPAGGAETISISNYGTPCINNGYSGTTYNMVRINGYIEYPSLHFF"
     gene            5997..6335
                     /locus_tag="PK252_13960"
     CDS             5997..6335
                     /locus_tag="PK252_13960"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT15858.1"
                     /translation="MYTSFGDRILLLRKKKKLTQEQLAQKIGIPVKELADYEGDAAFP
                     PFNIAVKIADVLDISLDFLACRIDEQPNMKWIKWAGDIDSLSDRTKEVLYAAVDALIN
                     YDKVKNIYKS"
     gene            complement(6378..7421)
                     /locus_tag="PK252_13965"
     CDS             complement(6378..7421)
                     /locus_tag="PK252_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15859.1"
                     /translation="MKQIFNIILGLSLFMFTTSLMIIKTDESEVVLFKKGMHDTILKE
                     VNKNSELKKNLPKKFDIIVVPSCYISFTLPIAKNYSDVTTISIDSTKGYGVLKSQDKV
                     FAVACRIGSSYSFSTNELLEPQKKTLKLIDYAVQHSANPFFVYFLKDEKYWDDAIGFF
                     DKEKISFIDENLHTYTNIEEIIKYRYGSVEKYLEITEDTKLKEQLLSKIANIEGAKAI
                     VKNDYVHWKNEFPQDTVGVLNLFIAEMDSITKLSAEQKALIKQKVIDRIRKYQLKTYT
                     GFGIPFYGEDISHEVQAVLTKNQLSKYLEQRALNSWLATQASNRVYNYLKREKNTPES
                     LLDSIYKQEVFGK"
     gene            complement(7443..>8024)
                     /locus_tag="PK252_13970"
     CDS             complement(7443..>8024)
                     /locus_tag="PK252_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOT15860.1"
                     /translation="IGLLSGLSGGGVGSAIGINAAASGGGTFIQGGQVPGGASPGSTG
                     NVYFTGNPTLTMGKQLPNTCVTSIMEYANHAFGGTTNQGVYDLYYMQNYDAQYYVNGV
                     DSRNINPFVNHFFKTSGFTSFNDAVNSGYPVMTDMFVSQQGNMINTHNVLVIGYNSGS
                     GNLIYMDPASGGASTGALSLFKTSYAIPLTGNR"
BASE COUNT         2423 a         1742 c         1328 g         2531 t
ORIGIN      
        1 cactactgta gcagcaacag ttgctatact cctcaggttg ctcttagtcc aactctaggc
       61 actgctaaac cacgagtacc ccgaagggtc aggagatatt cataaacttt atttttctat
      121 agtataatta ctttcaatat atggcattat tgagaacttg tccaaaaaat aataagaata
      181 tgttctatat aaatatttct ttctatcatt tattatacat ttttcattta aacctattgg
      241 atatagggta ttactctcaa caaatttttt tattttttct aatgaagctt ttgattcaat
      301 caactttcga aattctttat tttttttctt cagcgtttca ttgtttttcg taataagacc
      361 aatagtaaca aatctctcaa agcccttagc tttatataaa atactacaat ttacccaatc
      421 atttgatcga tttacactat caatattaat tgctattcca atattatcaa ttaaatcaca
      481 ataatttcca ctataacaat ttgacattac taaattagaa ctatcatttg aaaaatatat
      541 atatatttct ttagaattta ggataataga gtccccggca tattttaaca taaaagaaat
      601 aaaataaata gaatcttttt gtaaactttt cattaaagag cctgtaagta attcataatc
      661 acaacaattc caagaaaata aaaccaaacc acaataggca tctccatcaa ttgaaggatg
      721 ataccctaga aaattcattg gtacaccata aattttactt tgagaagata ttctttttga
      781 aaaataatca acagaagtac cattttgtat gtaccaattt ttagctaaaa atctatatgc
      841 attaggactt tcatcaacta tttcaaaaga tccatttcgc attagttctt gacaagttaa
      901 atcctcatat aaactaaaat agaataatat agctaaaaaa atgtgtttca taataaacta
      961 acttaatttt tataaaacgt aagataaaaa cctaaccctc catttaaccc ttcattattt
     1021 ctaaattgat ttggatcaag atttccacta ctatgataat ataatccttt acctggtata
     1081 ctaacttgcg caggaaacgt taaattacca aaaaattctc caaagtataa atcctgtcct
     1141 tcacttgaaa taaatgcgta agaattatca aataactgac ccttaaaatg tgcaattgta
     1201 ttttgagcat cttttggcat ctcaatattt ccagcaattg tatatgcccc tcccttattt
     1261 ctagtcatct gttttacaaa acttttctca aaataagaac tttgattctt tctcagattt
     1321 tctgctcgaa aagaaggtat acttttacct tgtctatcta agtaatacat ttgttgttcc
     1381 cctggagtag gtctataacc agcaggtgtt tttgtccagc cattattact aatcccaatc
     1441 gcactaccaa ctccaccacc agacaaacct gaaagtaacc caataccacc tccttcgccc
     1501 aatgcactgc caaagcttgg taaaagactg gctaaagttc tactacccgc accttcgaac
     1561 attgcctttg ttgtaataga ccaggatacg tctgtaactg tccctgcccc atgccaggta
     1621 tttaaataca aattatttaa accatagcta accgctccta ataccccggc agttgcaagc
     1681 ccggtagctg cacctatgca agctcctttt acaccattaa aaatagcatc acttaaagaa
     1741 cctttattta gaagtgtgcc tgcaaaacct cctgtaaagc ctccggcagc tccagccaag
     1801 aatgcacaag ctactgtgcc catccctgcg gtagctatag ttacacctgc aaccaccact
     1861 actgtagcag caacagttgc tatactcctc aggttgctct tagtccaact ccaagcccta
     1921 ctaaaccacg aatacccgct cggatctgta tacttcagcg gattgttgat acaatagcta
     1981 tacctgttgt agctttggct gttaaatggc tcctgcacgt acgggtcagg gctcataaac
     2041 cgccccaacc gtgggtcgta cacacggcca tccatattga ccagctcgaa gaggtcgata
     2101 tgctcgtggc cggtaaaacc acgggctgta ttggcagtaa tggccgcagg cttccatgta
     2161 acagggtcgc ggcggcaccc ccaggcatca tagctatact cttcgagcac ggttgtaccg
     2221 gcttcgttaa ccacatattg caaagaaccc aggtggtcgc gcaggcaata taccagctta
     2281 gtattggccg taaccgtact ttgggtgtat attgccatgg ctgcccctcc tgcaaatata
     2341 taatgagttt ctttaacttc gcccgaagga aggatttctt tttcgtatag cttgcctatg
     2401 tagtatttgg tggttacctg gccgggctac aaaagctttg cgttttcaga aaacgcaaag
     2461 cttttaggcc tgctcaaacc aaaccacgcc aagccttgat gtgccatgtt aaacggccgg
     2521 aggccggtta caacgcgacg tagcaggatg ctacggaccg cttttattgg caggctacga
     2581 tgaacgaggg ggcctcaact gctcccccta tggcgtgagt ctgcgacttg tgcctctttt
     2641 tattgaagtt tacaacttca gtttacccct taaatagttc aagtctctga cttggacaac
     2701 aaaatattac aattacaaca aataaagtaa aattttaacc ctactatatc tgccttatct
     2761 tatatagcag ttgcaaactg caagaattgt aggcacaagt cgcagactcc ataaggggga
     2821 cgcttgcctc ttctaacctt tttactaaac agcttcttac atacaacaat acaacatcct
     2881 tacttaacat cgtgcttact ttagctcttg ctcgttcgtt gttgttacag cacgcgtcac
     2941 agacgcgcta gctgggtttt gcctcacggc aagcaccctt gtcattcgct aacacttcct
     3001 ttcaactcgg cgtgtataag gacttacacc ttattagaaa tgggctatgc ccgacatact
     3061 taaacacaaa agccggattg ctccggcctc tgtatttatt tgtttatagg gtaagtaaaa
     3121 tctaattcta catcactttc cgatgttact gatacatcat caataaaata ataggccgct
     3181 tcaaccggga acagtggatt ctttttgtaa tgaggattta ttggaaaaat atcagtgtgc
     3241 tttttgtaat acttttcctt ttctctttca ttaaactgta cttctgtata tttttttaat
     3301 aatctaccag taatttcatc atcataaaac ttgccaagcg ttatgtattt ttcaccccca
     3361 ttagctttat aattacctgt aatttcaacc catgcagtgt catttaagag aatactatct
     3421 ttattgcttt gtacacttgc tgtaatttta ggttctaata tttcataata ataaggagga
     3481 gaccatgttt taaaaggatt tttagtttta gaaaaatata ctccgatgct ttttgttgca
     3541 aacctacatg aagcacctcc atatctgata aaaaaactca catgatacac tttgcctttt
     3601 tttagttcct tctttagtgt gccagtataa tgctccatgt atccatcata attcaaagga
     3661 taaagaccta tataagcatc tcctgtatgg gcaggatgga aaccaagctc agtatttggt
     3721 acttgtaaaa aataaaatgg agcattaaca tgaaaataat ccggagaaga ttcatcagga
     3781 acaaaccaat tcttggcaaa agaaatacca tcggcgatgc ttgttttggg caagtcggta
     3841 tattcttcaa aactagcatt caacacaaga ttttcctgtg ctgttgcaag ttttgttgtt
     3901 aataaggcta ttataaatat tgttttatgc atatcaagtt ctaaaagaag tgtaggcttg
     3961 gatactcaat atagccatta attctaacca tattataagt tgttccgctg taaccattat
     4021 ttatacaagg ggtgccgtaa ttcgatatac taattgtttc tgctccacca gctggtatag
     4081 ttgtccacgt agctttagga gcgtcacaga atagtacagg agtacgatta tctacagaga
     4141 tacttaaaca attgtctccg caaaaaccat tattatttac ttctatatct gttactgtaa
     4201 ctttttcagg tatggttata ttaagctcat caccaggact agtagcatta gttattttta
     4261 aaaaatctct tgtcttacca tagctaggtt ctgccttcgc ataggcattc tttacatctc
     4321 ttgctatgta tgaaccatca ctttttacta aaactggtat tctggcctga tttgtccaaa
     4381 tattattacc tgcacgatat gctttatagc ctccgattga accacctaat atgcctcctg
     4441 tgactgcacc gattgcagcc atattccagc cagtatttaa tgcctgactt ggggatgctc
     4501 tatttgctaa ggcagagcca aaacctccaa caaaaccacc tactgcaccg cctactgcac
     4561 ctgaaaaagc tccgccaact actccacctg atatacctgt tgcagaattt aatgccaatg
     4621 aagctcccaa cccagcagca ccaccggcaa gtccggctgc tgcaccaatc aagccaccaa
     4681 ctattcccgc tcctaagcat tgcccaacac ttcctccatt taatgccgta cctactacac
     4741 ctcctgtaaa accagccgct gcaccagaca aagtagcaac aagtaaaggt gaagctgccc
     4801 ctatagttaa tgctgtagct actcctgcac caattgctgc aacagcagtt acaataggtt
     4861 tccaattttt acctacccaa gaacaagccc tactaaacca tgagtacccc gaaggatcgg
     4921 tgaagcttag cgggttattc agacaatagc tatacctgtt gtagctttgg ctattgaacg
     4981 gggcctgcac gtacgggtca gggctcataa accgccccaa ccgtgggtcg tacacacggc
     5041 catccatatt gaccagctcg aagaggtcga tatgctcgtg gccggtaaaa ccacgggctg
     5101 tattggcagt aatggccgca ggcttccatg taacagggtc gcggcggcac ccccaggcat
     5161 catagctata ctcttcgagc acggttgtac cggcttcgtt aaccacatat tgcaaagaac
     5221 ccaggtggtc gcgcaggcaa tataccagct tagtattggc cgtaaccgta ctttgggtgt
     5281 atattgccat ggctgcccct cctgcaaata tataatgagt ttctttaact tcgcccgaag
     5341 gaaggatttc tttttcgtat agcttgccta tgtagtattt ggtggttacc tggccgggct
     5401 acaaaagctt tgcgttttca gaaaacgcaa agcttttagg cctgctcaaa ccaaaccacg
     5461 ccaagccttg atgtgccatg ttaaacggcc ggaggccggt tacaacgcga cgtagcagga
     5521 tgctacggac cgcttttatt ggcaggctac gatgaacgag ggggcctcaa ctgctccccc
     5581 tatggcgcaa gtctgcgact tgtgcctctt tttattgaag tttgcaactt cagtttaccc
     5641 cttaaatagt tcaagtctct gacttggact acaaaatatt acaattacaa caaatatagt
     5701 aaaattttaa ccctactata tctgccttat cttatatagc agttacaaac tacaagaatt
     5761 ataggcacaa ggtactgtca taagggggga tgaagagcca cggaatcagc cgaatgtaac
     5821 aattcaggct acagcctgat gaaatactta cgactaaggc agagccttag ccgaacgggg
     5881 gatgattgaa cagcttgacg aacccgacaa acaagccctt ttcaggatga ttgattgtat
     5941 gttgacaaag aataagttta aagatttctt tcaaaaaaat gtagctgcat tataacatgt
     6001 acacctcctt tggcgaccgt atcctattgc tccgtaaaaa gaaaaaactt actcaggaac
     6061 aacttgccca aaaaattggc attccggtta aagagttggc cgactacgaa ggcgatgctg
     6121 cttttcctcc tttcaatatt gctgtaaaga ttgccgatgt acttgatatt agcttagatt
     6181 ttttggcttg taggattgac gagcagccta acatgaagtg gattaaatgg gctggcgata
     6241 ttgattcttt gtctgataga acaaaggaag tgctttacgc tgctgttgat gctcttatta
     6301 attatgataa agtgaaaaat atctataaaa gctaaaaaag caaatccggc aaaatgccgg
     6361 atttgctact atttctatta tttaccaaaa acctcttgct tataaatgct atctaataaa
     6421 ctctctgggg tgttcttttc tctttttaga taattataaa ctctattaga agcttgggtt
     6481 gctaaccaag agtttagtgc tctttgttct aaatacttac tcaattgatt tttagtcaat
     6541 actgcttgta cttcgtgaga gatatcttct ccataaaaag gaatgccgaa ccccgtgtat
     6601 gtttttaatt gatatttccg gattctatct ataacctttt gttttatcaa tgctttttgc
     6661 tctgctgata gcttagttat tgagtccatt tcagcaataa acaaatttaa aacccctacc
     6721 gtatcttggg gaaattcatt cttccaatgt acataatcat tctttactat agcttttgcc
     6781 ccttctatat tagctatttt actcaataac tgctctttta actttgtatc ttctgttatt
     6841 tcaagatact tttcaacact gccatatctg tactttataa tttcctctat atttgtgtat
     6901 gtgtgcaggt tttcatctat aaatgagatt ttctccttat cgaaaaagcc aatcgcatca
     6961 tcccagtatt tttcatcctt aaggaagtaa acaaaaaatg gattagcaga atgttggacg
     7021 gcataatcta tcagtttcaa cgttttcttc tgaggttcta aaagttcatt agtagaaaag
     7081 gaataggatg aacctattct acatgctaca gcaaatactt tatcttgact ttttaaaact
     7141 ccatatcctt tagtagaatc aatactaatg gtagttacat cgctataatt tttagcaatt
     7201 ggtaaagtaa aggaaatgta gcaactggga acaactataa tatcaaattt ctttggtaaa
     7261 ttctttttta gttcactatt cttgtttacc tctttaagta tcgtatcatg cattcccttt
     7321 ttaaatagaa caacttcaga ttcatcagtt ttaattatca tcaagcttgt tgtaaacatg
     7381 aacaatgata atcctaatat tatattaaat atttgtttca taattaaata agattagatt
     7441 tattacctat tacctgttaa tggtattgca taactggttt taaatagtga taatgcacca
     7501 gtacttgctc caccacttgc aggatccata tatatgagat taccgctgcc tgaattatat
     7561 cctataacaa gtacattgtg agtattaatc atattgcctt gttgactgac aaacatatcc
     7621 gtcattacag gatatccgga atttacagca tcattgaaac ttgtaaagcc agaagtttta
     7681 aagaaatgat taacaaaagg atttatattt ctgctatcaa caccatttac atagtattgt
     7741 gcatcataat tctgcatata atacaaatca tatacacctt gattagtagt accgccaaaa
     7801 gcgtgatttg catattccat tatagaagta acacaggtat ttggtaattg ttttcccata
     7861 gtaagtgtgg gattgcctgt gaaataaacg ttaccagtgc ttccgggact tgctcctcct
     7921 ggaacttgac caccttgaat aaaagttccc cctccgcttg cggcagcatt aatcccaatc
     7981 gcactaccaa ctccaccacc agacaaacct gaaagtaacc caat
//
LOCUS       DAOSPF010000111         7994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_9016_length_7994_cov_6.886384, whole genome shotgun sequence.
ACCESSION   DAOSPF010000111 DAOSPF010000000
VERSION     DAOSPF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9016_length_7994_cov_6.886384"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..586)
                     /locus_tag="PK252_13975"
     CDS             complement(191..586)
                     /locus_tag="PK252_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15861.1"
                     /translation="MMQNKTIVIADYESDFAARLSGALRNYNFTVYTASDITEAKELC
                     LRFHPDVVIVDMLKHNDDAGIVLAHYLKKNRNNTPVMLISHVTANTGISFNVATESEK
                     KWICADVILDKNTDFPHILMEIEKLCTIS"
     gene            complement(591..2297)
                     /locus_tag="PK252_13980"
     CDS             complement(591..2297)
                     /locus_tag="PK252_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT15862.1"
                     /translation="MTFEIQINNKPVTVKRGETILEVLNRIGIKVPTLCSMKGFAPTG
                     MCRMCVIEVEGKENLIPACSFKVEEWMKIQTHSPRVVHARKTIVELLLANHPDDCLFC
                     ERNGNCELQTLAEELHVRERTIKGEKRKFYIDQSALSIVYDTDKCILCGRCIRVCRET
                     IGNHTFDFAYRGKRMSVLPALNKNLNHSNCLQCGQCIMVCPTAALSDKVNFSEFKVFF
                     HDVNKKAVAVYSPAVATTLAETLGLKSKASLHGILSAVFRKLGFDYIIDSSIGADLML
                     QMQCEEFISRLKANDKFPMVTSCCPSWVKYFEQLSIDNLHLLSSQKSSGQMAGHIAKR
                     YISKKDNIAVENIITVSIVPCTSRKYEAKRPEMVIDGITDTDAVITTRELLRMIKMNG
                     IDINNIEPEPWSGFFATNSSAGKLVNIAGGTTEAFIINLHYELTGKILDGLKPLNTRN
                     LKEYKEFSLNIGDHLINFVIVNGLSGINKVIDIIKEGKKDIHFIEVMVCQGGCIAGGG
                     QPLPASEESLKLRLKNIYEAVDSAAIRTCYENASLAEMLREEIIDKNNLRALFLKKEV
                     LL"
     gene            complement(2304..4235)
                     /locus_tag="PK252_13985"
     CDS             complement(2304..4235)
                     /locus_tag="PK252_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT15863.1"
                     /translation="MSISKELVKLILQKDKSVADNTIAQQIAVLRRESITKPVITVGS
                     GTCGMIAGANETFKAIEEYITDRGIEAQLQKVGCIGICSMEPVVEIHIPGKTRLFLKN
                     LTEEKVNDALDDAFHHIVPAEHTLMQIENGNLSAWPNVPNYNNVPFFALQNRIVLKNC
                     GLINPYDIDQYLALGGYKAFIKTIRNYTALQICDLVEESGLRGRSGGGYTTGKKWKTA
                     LFEQSEQKYLICNAEESDPGAFMDRAIIEGDPHRVIEGMAIAAYAIGATKAFVYIRSE
                     YTVAIQTLDTAIKQAKAAGLLGQDIQGSGFNFDISIRKGPGAFVCGEETALISSIEGD
                     RGMPETKPPFPAKQGLFNKPTVVNNVETLANIAEIINRGPSWFSAIGTEKSKGTKIFA
                     ISGKIHHTSLVEVAMGTSFRQIIFDIAGGIKDDKHFKAMLLGGPVGFCLSEKDLDLHV
                     DFDILRNHGLGVGSGGLIILDENNCIIDTLKYYMDFMQYQSCGKCIPCREGIKRLFEI
                     LTRITHKPLNSEDNSTLERIKGIIQLEEIAQVMKATSLCGLGQSVVNPILSALERFKH
                     EFEEHLYERKCQAWICKQLRTFTIDVDACTGCSLCARKCPTSAIIGSKRMPYFIIEEK
                     CIGCGYCIDACKFDAVFVK"
     gene            complement(4248..4715)
                     /gene="nuoE"
                     /locus_tag="PK252_13990"
     CDS             complement(4248..4715)
                     /gene="nuoE"
                     /locus_tag="PK252_13990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT15864.1"
                     /translation="MSSSAIAEILKNFQRNRSEALIPVLQEVQEADGYISELAVRQIS
                     EYLGIPSSKIYGVATFYNQFRFSPRGKFHIQVCFGTACHIMGSSTVLEVIERLLKIKD
                     GQCSKDGLFSLEVGTCTGGCGQAPVIVVNGVFYGKVTEERIKQIIAEMQDEAI"
     gene            4850..6715
                     /locus_tag="PK252_13995"
     CDS             4850..6715
                     /locus_tag="PK252_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT15865.1"
                     /translation="MTSDKLIRLDESKCKICYTCVRACPVKAIKVMVSGQQPVVDNDR
                     CIACGTCVTACNPRAITYRTDTELVTELLANHPNVVVSLDPALASEFNDITDYRKLAR
                     MLKQLGFKQIYEVSFGVDLVARRYKSLFHHFKGRYYITSCCPAVVSYVEKFHPGLINN
                     LAPIVSPMIAMGRVIRAKLKTDVKIVHVGPCIAAKNEAKLYEPNDSVDVVLTFEEIRK
                     MFKERQINESTLEYSHISEPVGYTGLLYPIANGILQAAEISENLLENIVVTGRGKTAM
                     IQYLSAFEPEIENIKTNFNLFFCKGCISGPGCTTQTNRLTRRSDVIGYANKRIKELNF
                     DTWQENVAKYNELELSRSFEPESCRMPEPPEEKVLAVLKSIGMPTREDEVDCGACGYK
                     TCRDFASGVIKGLTTTEMCAIFSQKNLTNYIKSLKATNEKLAQMQAAIKNSEKEARMG
                     QEAAQEASEVTSAMLQKLRSGVVFIDKNLKVIQANNNFINMLGEEVKEISEVIPGLAG
                     ADVKRLLPYAFCNMVNYVFTTHEDVLYKDIDTSVGIINVSIFPLKKGQIVGAILRDMH
                     QPEVLKAEIENRVSEVVEKNLEMVQKIGYLLGEGAAETERMLNSIIKSFKPKSEK"
     gene            6712..7899
                     /locus_tag="PK252_14000"
     CDS             6712..7899
                     /locus_tag="PK252_14000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT15866.1"
                     /translation="MISNLFIEASCKQIYCNGERVCGDVFHTVNVKEEGRRIIVLSDG
                     MGHGVKANILGILTATMAARFTVEHKETSQIAETILNTLPIDSEKKTSFSTFTVVDIE
                     ADGKTTILEYENPQCLVLRGDKVFQPQWNCLMLNTKKNVGREILCCTFQAQQDDRIIM
                     FSDGVTQAGMGSKALPLGWGTEAVHQFILAQVTKNMEIDASQLGNEVVNCALRYDGFE
                     SKDDISCAVVYFRKPRKILVCTGPPVDMDLDKDMARIFEEFDGKKVICGGSTSELIFR
                     ELKIDTSKLIEEEDPHNPPFYLLPGIEIITEGVVTLNAVYNTLNKMSLQNEGTIKGAV
                     GILANLLIESDDIKFLVGTKINQAHQSPDVPIDLEMRRTVVHRIARVLEQKYLKTVEM
                     TFM"
BASE COUNT         2469 a         1575 c         1568 g         2382 t
ORIGIN      
        1 taaatttcaa acttttcaaa gaactctttt agattttaat gggacagtaa tgagagattg
       61 ttgacattca ataaatatat ttaacccaga tttaatcata tagttcgact taataatgat
      121 taatttgggt ttattatata cagttttata ggttttatca ataaactcga gctgaggtta
      181 aaaaatcatc tcagcttatg gtgcaaagtt tttctatttc catcagaata tgagggaaat
      241 cggtattttt atcaaggata acatctgcac atatccattt tttttcgctt tcggtggcaa
      301 cattaaaaga aatgccagta ttagctgtaa catgactaat aagcattaca ggcgtgttgt
      361 tacggttttt tttcaaatag tgagcaagta caatccctgc atcatcatta tgttttagca
      421 tatcaacaat aaccacatcg gggtgaaacc tcaagcacaa ttcttttgcc tcggtaatat
      481 cagaagcagt atagactgta aagttataat ttcttaaagc tccactcagt cgagctgcaa
      541 aatctgattc gtaatcagct atgacgatgg tcttattttg catcatggca ttataataat
      601 acttcttttt tcaaaaacaa cgccctaagg ttatttttat caattatttc ttcgcggagc
      661 atctcagcaa gagaagcatt ttcgtaacaa gtacgaatag cagcgctatc aacagcttcg
      721 taaatatttt tcaatctaag tttgagagac tcttcgctgg caggtaatgg ttgtccacct
      781 ccggcaatac atccaccctg acataccata acctcaatga aatgtatatc ttttttacct
      841 tcttttataa tatcaatgac tttatttata cccgacaagc cattgacaat aacaaaattt
      901 atcaaatggt ccccaatatt gaggctaaac tctttgtact cttttaggtt cctggtatta
      961 agaggtttta ggccatcaag tatttttcct gttagttcgt aatgcaaatt gataataaaa
     1021 gcttcggtag taccacctgc aatgttaacc agctttccgg cactactgtt ggtagcaaaa
     1081 aaaccactcc agggttcagg ctcaatattg ttgatatcaa tgccattcat tttaatcatc
     1141 ctaagtagtt cacgggtggt gataacggca tcagtatctg ttatgccatc aataaccatt
     1201 tcgggcctct tggcttcata ctttcttgag gtacagggaa ctattgatac agtaatgata
     1261 ttttctacag caatattgtc ttttttagaa atataacgtt tggcaatatg tccggccatt
     1321 tggcctgatg atttttggga agataaaagg tgcagattgt ctatacttaa ctgttcgaaa
     1381 tattttaccc agcttgggca acaactggta accattggaa atttgtcatt ggcttttaac
     1441 ctggaaataa actcttcgca ttgcatttgc agcatcaggt cggcaccaat ggacgaatcg
     1501 atgatgtaat caaagccaag cttacggaat acagcagaaa ggataccgtg caagctggct
     1561 ttacttttca gccccagggt ttcggccaag gtagttgcaa ctgccggaga atagacagct
     1621 acagcttttt tgttgacatc gtgaaaaaaa actttaaact ccgaaaaatt aaccttgtca
     1681 gataaagctg cagtaggaca aaccatgata cattgtccac attgcaggca gttgctgtgg
     1741 tttagatttt tattcaaagc aggtagcact gacatgcgtt ttccgcgata agcaaagtcg
     1801 aatgtatgat ttccgatagt ttcgcggcat acccttatac agcgcccgca aagtatgcac
     1861 ttgtcagtat cataaactat agaaagggct gactggtcta tataaaattt tcgtttttcg
     1921 cctttaatag tcctttccct gacgtgcaat tcttcggcca aggtttggag ctcgcaatta
     1981 ccgtttcgtt cgcagaaaag gcagtcgtca gggtgattgg ccagcaaaag ttcaacaatg
     2041 gtttttctgg cgtgtactac acgtggggag tgggtctgaa tcttcatcca ttcctctact
     2101 ttaaacgagc atgcagggat aaggttttct ttcccttcaa cttcaataac acacatacgg
     2161 cacattccgg taggggcaaa gcctttcatg ctacataatg taggtacctt aataccaatg
     2221 cggtttagca cctccagtat agtttcacca cgtttgacgg tgacaggttt attatttatc
     2281 tgaatttcaa atgtcatgtt ggtttacttt acaaaaacag catcaaactt gcaggcatca
     2341 atacagtagc cacagccaat acatttttct tcaataataa aataaggcat acgtttactc
     2401 ccaataatag cagatgttgg acactttcgg gcacacaaag aacaaccggt acaagcatct
     2461 acatcaatgg taaaggtgcg gagctgctta caaatccatg cttggcactt gcgctcgtac
     2521 agatgctcct caaattcatg cttgaaacgt tctaaagcgc tgagaatggg gtttacaaca
     2581 ctttggccca acccgcacaa tgaggtagct ttcatcactt gggctatctc ttcaagttgt
     2641 atgataccct ttattctttc taatgtagaa ttatcttccg agttgagtgg tttatgagta
     2701 atacgggtta ggatttcaaa caaacgtttg ataccttcac gacaagggat gcactttcca
     2761 caactttgat attgcataaa gtccatataa tacttgagcg tatcgatgat gcaattgttt
     2821 tcgtcaagta tgataagccc tcctgaacca actccaagtc catgattacg aagtatatca
     2881 aaatcaacgt gcaagtcgag gtctttttct gataggcaaa aacctacagg gcccccaagt
     2941 aacatggctt taaaatgttt gtcatcttta atgcctcctg cgatatcaaa aattatttgc
     3001 ctgaaagatg tccccatagc aacttctaca agcgaggtat gatgaatttt tccagatatg
     3061 gcaaatattt tagtgccttt acttttttca gttccaattg cactaaacca agaagggcct
     3121 ctgtttatta tttcggcaat attagcaagg gtttcaacat tatttacaac agtaggttta
     3181 ttaaataagc cttgttttgc aggaaatggg ggcttcgttt cgggcatccc cctatctcct
     3241 tcaatactgc ttattagagc tgtttcttca ccacaaacaa aggctcccgg gccttttcta
     3301 atagaaatat cgaaattaaa accactccct tggatatctt gtcccagcaa accggcagcc
     3361 ttagcttgtt ttatagcggt atcaagagtt tgaatagcaa ctgtatattc agaacgaata
     3421 tatacaaagg ctttagtagc cccgatggca taagcagcaa tagccatacc ttctattacc
     3481 cggtgagggt cgccctcgat gatagctctg tccataaaag cccccgggtc gctttcttca
     3541 gcgttacata tcaggtattt ttgttcagat tgctcgaaca aagctgtttt ccatttttta
     3601 cctgttgtgt agccgccgcc ggagcgcccc ctaagaccgc tttcctcaac taagtcgcat
     3661 atttgtaaag cagtataatt acgaatggtc ttaataaaag ctttatagcc acctaaagca
     3721 agatattggt cgatatcata gggattgata aggccacagt ttttaagcac tatcctgttc
     3781 tgtaatgcaa aaaaaggtac attgttataa ttagggacat tgggccaggc agagaggttc
     3841 ccattctcaa tctgcattaa agtatgttca gcagggacaa tatgatgaaa agcatcatcc
     3901 aatgcatcat tcactttttc ttcagtcaaa tttttcaaaa acagccttgt tttgccgggt
     3961 atatgaattt ctacaacagg ctccatagag caaatgccaa tacatccaac tttttggagc
     4021 tgggcctcaa tgcccctgtc agtaatatat tcttcaatag ctttaaatgt ttcgttagca
     4081 ccggcaatca tcccgcaagt gccagagcca acagtaataa caggttttgt aatactttcg
     4141 cgcctgagca cagcaatttg ttgagcaata gtattgtcag caacactttt atctttttgc
     4201 aatatcaatt taaccaattc ttttgagata ctcatgtctg caaagtatta tatagcttca
     4261 tcctgcattt ctgcaatgat ttgtttaatt cgctcttcgg taacttttcc ataaaaaact
     4321 ccattgacaa caattaccgg ggcttgtcca catcctccgg tacaggtacc aacttcaagg
     4381 ctaaaaagcc cgtctttact acactgcccg tctttaattt ttagtaacct ttcaattacc
     4441 tctagcactg ttgatgagcc cataatatgg caagctgttc caaaacacac ctggatatga
     4501 aattttcctc gtggcgagaa cctaaattga ttgtaaaatg tagccacacc ataaatttta
     4561 cttgacggga tgccgagata ttcgcttatt tgcctgactg ccaattcgct tatatagcca
     4621 tcggcttctt gcacttcctg taaaacagga atcaaagctt cacttctgtt tctctggaaa
     4681 ttcttaagta tttctgctat tgctgaactg ctcatccgtt cttttacatt ttggtagaaa
     4741 acaaagatac aaaagattgt tattgatgaa acaatgttaa taaaataaca aattgtttct
     4801 atctttacaa caaatactaa gcaccgccaa atatctacaa tacagaaaga tgacatcaga
     4861 taaattaata cgtttagacg aaagtaaatg taagatatgt tatacctgtg taagggcgtg
     4921 tccggtaaaa gctattaaag ttatggtaag cgggcaacag ccagttgtag ataatgaccg
     4981 atgtattgct tgtggcacct gtgttacagc ttgcaatccc cgggctataa cttacagaac
     5041 tgacactgag ctggtaaccg aacttttggc caatcatcct aatgtagtgg tgtcattaga
     5101 cccggcttta gcttctgagt ttaatgatat taccgactat cgtaagctgg caagaatgct
     5161 caaacagctt ggtttcaaac aaatatatga agtatctttt ggcgttgacc ttgttgcccg
     5221 gagatataaa agccttttcc atcatttcaa aggaagatat tacataacat catgttgtcc
     5281 ggctgtggtt tcttatgtag agaaatttca tcccgggttg atcaataacc ttgcgcctat
     5341 agtgtcacca atgattgcaa tgggcagggt tataagggca aagctaaaaa cagatgtaaa
     5401 aatagtgcat gttgggccat gtatagctgc aaaaaatgaa gcaaagctat acgaacctaa
     5461 tgattcggta gatgtggtac ttacttttga agaaatcaga aagatgttta aagaacgaca
     5521 aatcaatgag agtacgttag aatattcgca tatttctgaa cctgtaggat atacaggact
     5581 tttgtaccca attgccaacg gaattttgca agctgccgaa atttcagaaa acctgttgga
     5641 aaacattgtg gttaccggca ggggcaaaac tgccatgatt caatatttga gtgcctttga
     5701 gccggaaata gaaaatataa aaaccaactt caatctcttc ttttgtaaag gttgtattag
     5761 tgggccaggt tgtacaaccc aaacaaacag gcttacacgc aggtcagatg tgataggtta
     5821 tgccaataaa cgtattaaag aacttaactt tgacacctgg caggaaaatg tagccaaata
     5881 caacgaactt gagttgtcgc gtagttttga gccggaaagt tgccggatgc ctgaaccacc
     5941 tgaagaaaaa gtgcttgccg tgcttaagtc tattggcatg ccaacccgtg aagatgaggt
     6001 ggactgtgga gcctgcggat ataagacttg tcgtgatttt gcatcggggg ttatcaaagg
     6061 gctaacaaca acagagatgt gtgctatatt ttcgcaaaaa aacctaacca attatatcaa
     6121 atcacttaag gcaacaaacg aaaagcttgc ccagatgcag gctgctataa aaaactcgga
     6181 aaaagaagcc cgcatggggc aggaagctgc ccaggaagcc tcagaagtta cttcggcaat
     6241 gcttcaaaaa ctcaggtctg gagtagtgtt cattgataag aacctgaagg ttattcaggc
     6301 aaacaacaac tttatcaata tgcttggcga agaggtaaaa gaaattagtg aagtaatacc
     6361 gggtttagcc ggtgctgatg taaaacgcct tttgccttac gcattttgta atatggtgaa
     6421 ttatgtattc actactcacg aagatgtttt gtataaagac attgatacaa gtgttggcat
     6481 tattaatgtt tcaatatttc cactaaaaaa aggccagata gttggggcaa tattacgcga
     6541 tatgcaccag ccagaagtat tgaaagccga aattgaaaac cgtgtaagcg aggtggtgga
     6601 gaaaaacctt gaaatggtgc aaaaaattgg ttacctgctt ggcgaaggcg ctgccgaaac
     6661 tgaacgtatg ctcaactcca tcattaaatc attcaaacca aagagtgaaa aatgatttca
     6721 aacctcttta tagaagccag ttgcaaacag atatactgta acggggagcg cgtttgtggt
     6781 gatgttttcc atacggtaaa tgttaaagag gaaggaaggc gcatcatagt actttctgat
     6841 ggtatggggc acggcgtaaa agcaaatatt cttggtattc ttacagctac aatggctgcc
     6901 cgttttacgg ttgagcacaa agagaccagc caaattgcag aaactatcct gaatacattg
     6961 cccatagatt cagaaaaaaa aaccagcttt tcgaccttta ctgtagttga tatcgaggcc
     7021 gatggaaaaa ctaccatttt agaatatgaa aaccctcaat gccttgtatt acgaggcgat
     7081 aaagtatttc agccccaatg gaattgtctg atgttaaata ccaaaaaaaa cgtgggtcgt
     7141 gagatattgt gctgtacatt ccaggcgcaa caagacgata gaattatcat gttctccgat
     7201 ggtgttaccc aggctggtat gggaagtaaa gcattgcctt taggctgggg gacagaagcc
     7261 gttcatcaat ttattttggc ccaggtaaca aaaaacatgg aaattgatgc atctcagcta
     7321 ggaaatgaag tagtgaattg tgctttacgt tatgatggat ttgaaagcaa agatgatatt
     7381 agctgcgccg ttgtttattt ccgaaagccg cgtaagattt tggtttgtac cgggccacct
     7441 gtagatatgg atttagacaa agacatggct agaatatttg aagagtttga cgggaaaaaa
     7501 gtgatttgtg gaggttctac cagcgagctt atttttcgcg aactaaaaat agacacctca
     7561 aagcttattg aagaagaaga tccccataat cccccttttt acctgctccc agggattgaa
     7621 attatcaccg aaggggtcgt cacgctgaat gctgtttata acacactaaa caaaatgtct
     7681 ttacaaaatg aaggaaccat caaaggagct gtaggcattt tggctaacct attgatagaa
     7741 agcgatgata ttaagtttct ggtaggcact aagattaacc aggctcatca aagcccggac
     7801 gtccctattg atttggaaat gcgccgaaca gtagtacacc gtattgccag ggttttagag
     7861 caaaaatacc taaaaacagt tgaaatgact tttatgtaaa ataaaataaa ttacaaaaca
     7921 atgaattatc cttttgctat gttgaactaa atccctacgg ggatagcttc attttagaac
     7981 aaatataatt gaat
//
LOCUS       DAOSPF010000112         7806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_9306_length_7806_cov_7.141272, whole genome shotgun sequence.
ACCESSION   DAOSPF010000112 DAOSPF010000000
VERSION     DAOSPF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_7806_cov_7.141272"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1200)
                     /locus_tag="PK252_14005"
     CDS             complement(94..1200)
                     /locus_tag="PK252_14005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT15867.1"
                     /translation="MASRIVLSVTNDMVIDQRVHRIASTLANAGNDVLVIGRYTGVWP
                     DFSDRPYKVHLMRLWFKKGFLFYANYNVSLFFRLLRTKCTILTANDLDSLPANWMAAK
                     LKRVALFFDSHEYFPEVPEVVNRKWVKRFWTFIEKTLVPRANYHYTVCQSIADLYKSK
                     YNLDFKVVRNVPLLKDEENREVEIERNENIIMYQGAINVGRGIENIIDAMPMIPDAKL
                     VIAGGGPLENDIKKRIQESPARERIKYLGRLPFHQLHQYTIQAQIGISIEENLGLNYY
                     YALPNKLFDYIQAGLAVVTSDFPEMKRVVDEYHIGTTIDNRSPENLANTINPILADKK
                     QLSAWQNNARAAAVELCWEKEQNILLSIYKAVNA"
     gene            1784..2701
                     /locus_tag="PK252_14010"
     CDS             1784..2701
                     /locus_tag="PK252_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15868.1"
                     /translation="MKKVKYFYVFLGIILLSILFYIYKAYFIIDKNEDSWTVGYGNIN
                     NLEDIDSIIEKKLLIKDIQSDSTFNFIADPFIFKDSTGIYVFVEQFIKKNGDISVFYS
                     KDTINVKFKYLGIVLDEDFHLSYPQVFAYNKHIYMLPETQKSGKVILYKSVKFPLTWE
                     KCRVLLKYDNIKDPTLLQHNSKIYLFGCQKGKLYCWISDNLFSDFKRIDKVLLLGTES
                     RPGGRIFKYDNKIIMPIQNSSKGYGSGLSLYEIIIDGNDNIKMKPYKRWFLKPHEKSI
                     YFSHGMHHFDVINLNSNLFVVYDGKKNKQ"
     gene            complement(2852..2924)
                     /locus_tag="PK252_14015"
     tRNA            complement(2852..2924)
                     /locus_tag="PK252_14015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2889..2891),aa:Lys,seq:ctt)
     gene            complement(2985..4268)
                     /locus_tag="PK252_14020"
     CDS             complement(2985..4268)
                     /locus_tag="PK252_14020"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT15869.1"
                     /translation="MEKNIKKVINAWCSYDIANSAYNLVITSTIFPIYYHEVTQKVIS
                     FSFPFDKILTIRNTVLYDYTIAVAYLLIIMLLPLLSGIADCGGYKKRFMRFFTLLGAI
                     SCISLYGFNANHLAIGLLGAAFAVIGFAGSLVFYNSFLPVIASPEHHDKISARGFSWG
                     YAGSVLLLLLNLIIIFNYQSLGFSSALNAMRFAFITVGIWWIAISSFSFYYLPEEVKE
                     KRFHLSNISKGYDEIIKVFRKVKQAPSLKWFLLAFFFLSMGVQTIMLVATMFGKEEIH
                     LSGESLIVTILLLQILGIIGATLFAKVSERKGNKASLIIMLVMWISICIVAFFIKTQA
                     QFYGLAAAVGLIMGGIQSQSRSTYSKLIPATDTASYFSFYDVCEKFAIVLGMFFFGLA
                     EHYMGNMRWSALLLSIFFIASLLIISRISLTKKSK"
     gene            complement(4279..5397)
                     /locus_tag="PK252_14025"
     CDS             complement(4279..5397)
                     /locus_tag="PK252_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT15870.1"
                     /translation="MPNIIQNIAFIVSFSFVFNLTVFAQPYKTDAFVIEKAIKVTEIK
                     NQFRSNTCWSYATIDMLEAELLKKGKKDIHLSEMFVVYNVYLEKAEKYFRMHGKNNLS
                     GGGALFDVISAIEKYGIVPYEAYSGLINGMQYNDHVALDNQISEKMKKVISSEQVSMD
                     WKKDIISILDNNLGTPPSEFTFQQVQYTPKSFAQWLNIKSSNYILFSSFIYKPLYKNF
                     VVEVPDNWAMGQAFNVKLDEMINIIEYSINNGYTVAATIDNSESGFNWNKGIVRVKDG
                     DPDLDSEPKITAQMRQKAFDEYQTTDDHSLLIMGIALSKTGERYFLAKNSWGTENTKL
                     GGYLYISEAYIRYKVLTILVNKSGVPGDILMQYNNTCN"
     gene            complement(5442..7370)
                     /locus_tag="PK252_14030"
     CDS             complement(5442..7370)
                     /locus_tag="PK252_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT15871.1"
                     /translation="MKQILLIILIITLSFVLVSINAQQLSTKSEKALKLYELALNQMQ
                     MKDYGATIKILNNAVKTDSSFTEAWLLMADAADMAGNYQLSAKACNAAIKYGGTQYKI
                     VYLFAAQANYRIGNYATAIDMAEKYIEAKSYSKNQLTEANKVIEYAKFAMEAIKHPVK
                     FNPVPLGDSINTKYDEYWPCITGDDSTLFFTRRIPRTKEHERFRDTVQEDIFVSNLNK
                     NGWGKARGISINTENNEGAQTITADGQQMFFTACDRADGYGKCDIYYCKKEGNNWSTP
                     QNLGNIVNSGYSETQPSVSSDGRTLYFASNRPGGKGKQDIWVTYLDSSNQWTKPVNLG
                     DSINTNDMEESPFIHADNTTLYFSSKGWPGLGASDIFISRKKKDGWSSPKNLGYPINT
                     YNNEVGLIVNAAGDKAYYTSDQDSGMKKNIYSFALSENTIKPGKTLYFKGKVYDAVTG
                     KPLGANFELTDLAFKTVVSKNISNTITGEFLVCLPSGKDYGLNINKNEYLFYSENFTL
                     SDTISTNKPVIKNIPLQPIQKGKGVVLKNIFFTTASYELNEKSSVELMQLVNFLKQHN
                     TLKIEVRGHTDDTGDAIANITLSENRAKAVTDFLTTHGIDISRVKYKGFGETMPIATN
                     KTPEGRAQNRRTEFVIIE"
     gene            complement(7461..>7806)
                     /locus_tag="PK252_14035"
     CDS             complement(7461..>7806)
                     /locus_tag="PK252_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOT15872.1"
                     /translation="GNTLEALIGAIYLDKGYEKTKKIVINRIVKQLISFDELEKREFD
                     FKSRIIEWAQKSKSEIVFESQEDYTGKSNTPIFISRIVIDNQILGEGRGTSKKEAEQN
                     AAEQALTYIDVK"
BASE COUNT         2380 a         1355 c         1422 g         2649 t
ORIGIN      
        1 tgtagtaatt agcaatgata tattatctgt gagtcgaaaa atgtttaaaa aatttttttg
       61 agaaatattt aacaaataaa aacaacgttt aggttaagcg tttacagctt tataaattga
      121 caaaagtata ttttgttctt tttcccagca aagttcaaca gcggcagcgc gggcgttgtt
      181 ttgccaggcg gatagttgtt ttttatccgc taaaataggg tttattgtat tggccaggtt
      241 ttcggggcta cggttatcta tggttgttcc tatatgatac tcatcaacta cccttttcat
      301 ttctggaaaa tcagaagtaa caacggccaa acctgcctgt atataatcga aaagcttgtt
      361 gggcaaagca tagtagtagt taagcccaag gttttcttct atcgaaattc caatctgcgc
      421 ttgtattgta tattggtgaa gttgatgaaa tggcaaacgc cctaagtatt taatccgttc
      481 gcgggcaggg ctttcttgta tccttttctt tatatcgttt tccaaggggc ctcctccggc
      541 aatgacaagc tttgcatcag gtatcatggg catagcgtct attatgtttt cgatgcctcg
      601 gccaacattg atagcgccct ggtacatgat aatgttttcg tttcgctcga tttcaacttc
      661 tctgttttct tcatctttca ataaaggaac attgcgaacc actttaaaat cgaggttgta
      721 ttttgatttg taaaggtcag caattgactg gcaaacggta taatgatagt tggcccgcgg
      781 aacaagcgtt ttttcaataa aagtccagaa ccgcttaacc catttgcggt ttaccacttc
      841 gggcacttcg gggaaatatt cgtggctatc gaaaaacaag gcgactcgtt taagctttgc
      901 tgccatccag ttggctggca ggctgtccaa atcgttggct gtcagtattg tacattttgt
      961 cctgagtagc ctgaaaaata aagaaacatt ataattggca tagaataaaa accctttttt
     1021 gaaccaaagc cgcatcaggt gaaccttata aggcctgtct gaaaagtcag gccaaacgcc
     1081 tgtatatcgc ccaataacca gaacgtcatt tccggcattg gctaatgtag atgcaatgcg
     1141 gtgtacacgc tggtcaataa ccatgtcatt ggtgacgctc aatacaatgc gagatgccat
     1201 aataaaatgt ttgcgctaag gtaagaaaag gatttatagt aaactatata ggttgtaata
     1261 gttgattgta tttctttttc attttaatgc aatgcaaatt ttacaaattt tatttgttta
     1321 cgcaggtagt agcccacgca taaagaattc ttcaaatatt gccctttagc tttgtaagct
     1381 atttgaaatg ctccgtaatt ggcttgccta aaatgacaaa atatcatact ggttaattgc
     1441 ccccggcttt caatgctttt attaaccttg ttgcagttgc attttttaac aatatccgaa
     1501 atatgttttt gaagcaacaa acttttaatt ttagaaaaaa gagataattt tgtcatagga
     1561 gttggttttt gtggttattg taaaattatc ttttaccgga taatctccaa ctcctttttt
     1621 ttacaaattg tacagatgtg aataatatgt gaaactctca tgattgtagg caaagagaaa
     1681 ctccatttat aaaatccggg tgttttatct gcttacattt aaataaatag taaatattaa
     1741 tatcttaaac aactgttttt tagcaagtta aaaattcata cggatgaaaa aagtaaagta
     1801 cttttatgtt tttttgggga taattctttt atccatactt ttttatattt ataaggcata
     1861 tttcattatt gataaaaatg aagattcttg gactgttgga tatggcaata ttaacaactt
     1921 agaagatata gattctataa ttgagaaaaa attacttata aaagatattc aaagcgattc
     1981 aacgtttaat tttattgcag acccttttat atttaaagat tcaacaggta tatatgtatt
     2041 tgttgaacag tttattaaaa aaaatgggga tattagtgtt ttttattcaa aagacacaat
     2101 caatgtaaag tttaaatatt taggtattgt acttgatgaa gactttcatc tttcgtatcc
     2161 ccaagttttc gcgtataata aacatattta tatgttacct gaaactcaaa aatcaggaaa
     2221 agttattcta tacaaatcgg ttaagttccc attaacgtgg gaaaagtgca gagtgttatt
     2281 aaaatatgat aatattaaag atccaactct actgcaacac aatagtaaaa tttatttatt
     2341 tggttgtcaa aaaggaaagc tctattgttg gatatctgat aatctttttt ctgatttcaa
     2401 acggattgac aaagtgcttc ttttggggac agaatcaagg cccgggggaa gaatctttaa
     2461 atacgataat aaaataatta tgccaattca aaatagttca aaaggctatg gaagtggact
     2521 ttcattatat gaaattataa ttgatggtaa tgataatata aaaatgaaac catataaaag
     2581 atggttttta aaaccacacg aaaaaagtat ttatttttct cacggtatgc atcatttcga
     2641 tgtgataaat ttaaatagta atttatttgt ggtttatgat ggtaaaaaaa ataagcaata
     2701 acaagaagtg atgtttttta tctggcaaga gttacaaagc tttacatttt gcaagatacc
     2761 cgtttatcag tacacgtttt ttatatcaca ggtgcatttt gctcacaaaa aagcggtaca
     2821 agtaaagctt tgtaccgcac gctgccattt gtgacctcgg agggattcga accctcgacc
     2881 cattgattaa gagtcaattg ctctaccagc tgagctacga agtcaattct tttttgagat
     2941 tgcaaaagta aaagattttt tttctccaac gaaaaaaaca gcaactattt tgattttttc
     3001 gttaggctaa tccttgaaat tattaataat gaagctataa agaatattga taaaagcaag
     3061 gcgctccaac gcatattgcc catatagtgt tcggccagtc caaagaaaaa catacctaag
     3121 acaatggcaa acttttcgca tacatcataa aaactgaaat acgaagcagt atcagttgct
     3181 ggtatcaatt tggagtatgt tgaacggctt tgcgattgta ttcctcccat gataagccct
     3241 actgcagcag caagaccata aaactgggct tgggttttga taaaaaatgc aacaatacaa
     3301 atgctaatcc acatcacaag catgataatc agtgaagctt tgttcccttt acgttctgaa
     3361 accttagcaa acagagttgc tccaatgata ccaagtattt gtaaaagcaa aatagttaca
     3421 ataaggcttt cgcccgaaag gtgtatttct tctttgccaa acatggtagc tacaagcatg
     3481 atggtttgta cccccatgga aagaaaaaag aaggccagta aaaaccattt gagggatgga
     3541 gcttgcttca cttttctgaa tacttttata atctcgtcat atcctttgga gatgttggac
     3601 aaatgaaacc ttttttcttt tacttcttct ggtaaataat agaaagaaaa agaagaaata
     3661 gcaatccacc aaatgcccac ggtgataaat gcaaaacgca tagcatttaa tgctgaactg
     3721 aagcccagag attggtaatt aaaaataata atcaggttga gaagcaaaag caaaacactt
     3781 ccggcatatc cccatgaaaa accacgtgca ctaattttgt catggtgctc gggagaggca
     3841 atgactggca aaaaagaatt gtagaacacc agcgagccgg caaagccaat aacagcaaat
     3901 gccgcgccca gcaaccctat agcaaggtga ttggcgttaa atccatacaa tgagatacaa
     3961 gagatagctc caagtagtgt aaaaaacctc ataaaccgct ttttataacc accacagtct
     4021 gcaatgcccg aaagtaatgg taacagcata atgataagca gatatgcaac agcaatagtg
     4081 taatcgtata aaacggtatt gcgtatagtc aatattttat caaatgggaa cgaaaagctg
     4141 atgacttttt gcgttacttc atgataataa atgggaaaaa tagtagatgt gataactaaa
     4201 ttatatgctg agttggctat atcgtacgaa caccaggcat taattacttt cttgatgttc
     4261 ttttccatga gttgctggtt aattgcatgt attgttatat tgcatcagga tatcccccgg
     4321 aactcctgat ttatttacta aaatggttaa aactttatag cgaatatacg cttccgaaat
     4381 atacaaatat cctcctaatt ttgtgttttc tgtcccccat gaatttttgg ccagaaaata
     4441 cctttcaccg gttttgctta aggcaatacc cataattaat aagctgtggt catcagtggt
     4501 ttggtattcg tcaaaagcct tttgccgcat ttgagcagta atttttggct cgctgtctag
     4561 gtcggggtct ccgtctttta cccttactat acctttgttc cagttaaaac cactttctga
     4621 gttgtctatt gtggcagcaa ctgtgtaacc gttgttgata gagtattcaa tgatattaat
     4681 catttcgtcc agttttacat taaaggcttg gcccattgcc caattatcag ggacttctac
     4741 aacaaagttt ttatataaag gcttatatat aaacgaggaa aaaagaatat aattggaaga
     4801 cttgatattt agccattggg caaaagattt aggagtgtat tgtacttgtt gaaaggtaaa
     4861 ttctgaaggg ggggtgccca ggttgttatc taaaatgctt attatatctt ttttccaatc
     4921 cattgatacc tgttctgaag aaatgacctt cttcattttt tcgcttatct gattgtctaa
     4981 ggcaacatgg tcgttgtatt gcattccgtt gattagtccc gaataagctt cataggggac
     5041 aatgccatat ttttcaatag cacttattac atcaaataaa gctcctcctc cagaaaggtt
     5101 gtttttacca tgcatcctga aatatttttc tgctttttcc agataaacat tgtaaactac
     5161 aaacatttcg gaaagatgga tatctttttt gccttttttc aatagttcgg cttccagcat
     5221 gtctattgta gcataactcc aacaggtatt ggaacgaaac tggtttttta tttcagttac
     5281 ttttatggct ttttcaataa caaaggcatc tgttttgtat ggttgggcaa agacagtaag
     5341 gttaaataca aaactaaaag acacgataaa ggcaatattt tgaataatgt ttggcaagtg
     5401 tattttgttt tttgattaaa aacatgttta aattacaaca attattcaat aattacaaat
     5461 tcggttcgtc tgttttgggc cctgccttct ggtgttttat ttgtggctat tggcattgtt
     5521 tctccaaagc ctttatattt tacccttgaa atatctatac catgagtggt taaaaagtct
     5581 gtcactgctt ttgcccggtt ttcgctaagc gttatattgg ctattgcatc accagtgtca
     5641 tcggtatgtc cgcgtacttc aattttcaag gtattgtgtt gcttcaaaaa attcactaat
     5701 tgcattagtt caacacttga cttctcattt agttcatacg aagctgtagt aaagaaaata
     5761 ttttttaata ctacgccttt gcctttttgg ataggttgta agggtatgtt ttttattacg
     5821 ggcttgtttg tactgattgt atcactgagg gtaaagtttt cggagtaaaa caaatattca
     5881 tttttattga tgttcagacc gtaatctttt ccacttggta aacaaactaa gaattcacca
     5941 gtaattgtat tagaaatatt tttgctcacc acggttttaa aggccaggtc ggttagttca
     6001 aagttggcac ccaatggttt gccggtaacg gcatcataaa ccttaccttt aaaataaaga
     6061 gttttaccgg gttttatagt attctcagat aacgcaaagg agtaaatgtt ttttttcatt
     6121 cctgagtcct ggtctgaggt gtaataagct ttatctccgg cagcattgac aattaggcct
     6181 acttcattgt tgtatgtgtt aattggataa cctaagtttt tgggcgaact ccagccgtct
     6241 ttctttttac gactgataaa aatatcagat gctcctaatc ctggccagcc ttttgaacta
     6301 aaataaagcg ttgtattatc tgcatgtata aaaggagatt cttccatatc atttgtatta
     6361 atgctatctc caaggtttac tggttttgtc cattggttgc ttgaatcgag gtatgtcacc
     6421 catatatctt gtttgccttt tcctcccgga cggttgctgg caaaatacaa ggttcgtcca
     6481 tctgaagaaa cagaaggttg agtttcgcta taacctgaat ttactatatt cccgaggttc
     6541 tgtggagtgc tccaattgtt cccttctttt ttgcaataat aaatatcaca tttaccataa
     6601 ccatcagccc ggtcacaggc tgtaaagaac atttgctgcc catcggcagt aatagtctgg
     6661 gccccttcgt tgttttctgt attaatgctg ataccgcggg cttttcccca tccgttttta
     6721 tttaggttac tgacaaatat atcttcttga acagtgtccc ggaaacgttc atgttctttt
     6781 gttctgggaa ttcttcgggt aaagaacaaa gtagaatcat ctccggtgat gcatggccaa
     6841 tattcatcat attttgtgtt tatcgaatct ccaaggggca cagggttgaa tttgacagga
     6901 tgtttaatgg cctccatggc aaactttgcg tattcaatca ctttattggc ttccgttaat
     6961 tggtttttcg aatagctttt agcttcgata tatttttctg ccatatcaat ggctgtggca
     7021 taatttccaa tccgataatt ggcctgtgct gcaaacaagt aaacaatttt gtattgtgta
     7081 ccaccatatt taattgctgc gttgcatgct ttagctgata actggtagtt tccggccata
     7141 tcggcggcat cggccattag tagccatgct tctgtaaaac tgctgtctgt tttaacagca
     7201 ttattgagga ttttaatagt agcgccataa tctttcattt gcatttggtt caaagcaagc
     7261 tcgtaaagtt ttaaggcttt ttcagatttt gtagatagct gttgtgcgtt aatgcttacc
     7321 agcacaaaag acaatgttat gattaggatt attagtagga tttgtttcat gctttgtagt
     7381 ttattatcat caaagataaa catacaaagc tatttaaaac aataatagaa caaaaagcgc
     7441 aaatctgaag aaaagaaggg ttactttaca tcaatgtaag taagcgcttg ctctgcagca
     7501 ttttgttcgg cttctttttt tgaagtaccg cgcccttcgc ctaatatttg attatcaata
     7561 acaatgcgcg aaataaaaat tggggtattg gattttcctg tatagtcttc ctggctttca
     7621 aatactattt cgcttttact tttttgcgcc cattcaataa tacggctttt aaaatcaaat
     7681 tcgcgttttt caagttcgtc aaaactgatg agttgcttga caatacggtt tatgacaatt
     7741 ttttttgttt tttcataacc tttgtccagg tatatagcgc caattaatgc ttccaatgta
     7801 ttacca
//
LOCUS       DAOSPF010000113         7582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_9685_length_7582_cov_5.425535, whole genome shotgun sequence.
ACCESSION   DAOSPF010000113 DAOSPF010000000
VERSION     DAOSPF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_7582_cov_5.425535"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK252_14040"
     CDS             complement(<1..370)
                     /locus_tag="PK252_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOT15873.1"
                     /translation="MSKVGQIERITQNRIVALFQSELEYRYLGNWEERENNSNVDEAL
                     LTAFLTKKGYTKNQINKALYEFYKAVNINTHDDLYQANKEVYSLLRYGIKVKEEAGEN
                     KETVYLIDWKNPLENDFAIAE"
     gene            complement(367..1677)
                     /locus_tag="PK252_14045"
     CDS             complement(367..1677)
                     /locus_tag="PK252_14045"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15874.1"
                     /translation="MIVVSEQYGYKNPLPIDNIDKYSDILNNKSYCRSIQRGDNKTYC
                     YKIDHFPDVENPYRLEASYFVGIDWVVENELAINIKPKMSDTSTEINFLKMLFDILKE
                     SENYNYLDQLCEIDFNKPTITIEQRQDILTPLLIIQYLNILKKIVQKGLRKTYYPITK
                     NLNAKIKGRILINETIKKNHFNNKMLYNCCQFSEFGVNSIENKVLKKALEFSISALKN
                     LKGIDTSYLNEMLNYIQPAFENVDSKVSIEELKNIKPNNFYKEYIQALIFARHILKRY
                     GYNISSVNSLSVKTPPFWIDMSKLFELYIFSKLKERFNKSKEVTYHKKFNYLEPDFII
                     KTYDGLLKMVVDAKYKPQYQNGKVNVDDIRQISGYARLKKIYSFLEIEEKTVIDCLVI
                     YSSQSAGRKDFIADNFQLEEEKEYNRFFKIGIELPENVFEGMTT"
     gene            complement(1680..3497)
                     /locus_tag="PK252_14050"
     CDS             complement(1680..3497)
                     /locus_tag="PK252_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT15875.1"
                     /translation="MKFKDLADLVNQVNATLGEFCDEFGSKRKELAGLGKVPKRRILF
                     KLTDQSRDWAINEGGGTELQYHIAFVVDDLEIRYGLGFNTQYVPFANEKSSVDYMRPY
                     MFGFLKNEKKVRGILPDYTFVYGDIQQLKAPQNNQYTLFGKSISVKNERNGFLIDDDA
                     FESMISDFKKQFEAYQIIFSDKNHFSEAAMNNQSIIDVLTHKGQIILQGPPGTGKTYT
                     AKDIAEQMLFSEVSEDKTIQKDRLENTEQFKFVQFHPAFSYEDFVRGIVAESKGISIE
                     YKTKNKLMVDFANRALRNYENSRKDKAQLKEENWIQENFLDFSDFVTDEIEKSNGKLL
                     LKGTTTYLREVENDCFRYTGDNWSNIAGNRMRFEDIIKMYRANVKTRQEIKKMTGISG
                     LAIQHASYYMRVLDMFYSFMDTKKKPDIDIPEIEEKKYVLIIDEINRANLPAVLGELI
                     YALEYRGEKVESMYPVDDDNTLIIPPNLYIIGTMNTSDRSVGHIDYAIRRRFAFIDVL
                     PKILDIENFELELFQQVSALFIQNFDAYALDTTVELIPSEHLSEEFRPEDVWLGHSYF
                     IKGEGDFSIRKSYEIIPILKEYLKDGILKQSAETLIRAL"
     gene            complement(3497..4675)
                     /locus_tag="PK252_14055"
     CDS             complement(3497..4675)
                     /locus_tag="PK252_14055"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOT15876.1"
                     /translation="MMDNEQLPKGYKVTEVGIIPEDWEVYILGDLLLRIADVDHYMPK
                     TEKYGIPYLMTGDIKDYASEIDFDNCKKISKDDFEKLSKKIRTLKSDIILARYATVGS
                     VSYVDIDEEFVVSYSCVTIKPDKSKLNDVFLFHYFKSNIFGLEVKNKVNANIQDNVGI
                     GDLMKMKIPLPPTKSEQTAIATALSDADALINSLEKLIAKKRNIKQGAMQQLLKPKEG
                     WEVKKLGEVAEIYQPQTISQDVFIDDGYLVYGANGIVGKYHLYNHETWQTTITCRGST
                     CGTVNRTVGKSWITGNAMVMNVDKNTNIDKLFFYYILTKQDFSQCITGSGQPQIVRNP
                     LFEFKVSIPKTNEEQTRIATILSDMDNEINALETKLEKYRKIKLGMMQVLLTGKVRLV
                     "
     gene            complement(4672..5535)
                     /locus_tag="PK252_14060"
     CDS             complement(4672..5535)
                     /locus_tag="PK252_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOT15877.1"
                     /translation="MEIVKQQHIESKIVMLRDVQVILDKDLASFYEVKPIRLREQVKR
                     NPNRFPPDFVFQLTENEVDSLVSQNAIPSKQSLGGHLPYVFTEQGVAAVAGVLKSEKA
                     AEVSITIARAFVAMRKFLFQNASVFQRLDRIELKQIQTDEKFERIFKALESGQATPDM
                     GIFFDGQVFDAYTFAVNLIKSATQSIVLIDNYIDESVLTLLSKRNIGVSASIYTKEIN
                     KALQLDVKKHNAQYPSIEIKTFANSHDRFLIIDHKELYHIGASLKDLGKKWFAFSRMD
                     SLVNELLKRIK"
     gene            complement(5526..>7582)
                     /locus_tag="PK252_14065"
     CDS             complement(5526..>7582)
                     /locus_tag="PK252_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOT15878.1"
                     /translation="NRAGGDDILGDAFEYLMKNFATESGKSKGQFYTPSEVSRVLAKV
                     LGITDPKNSTTFYDPTCGSGSLLLKVIDEADGKGSIYGQEKDVATAALARMNMILHGI
                     EGHEIHRGQSTLSDPFFKNKDGYLRTFDYVVANPPFSSKNWVNGFDPQNDIYGRFEKD
                     EEESAKTGKTIYKTPPEKNGDYAFLLHILKSLKSTGKGACILPHGVLFRGNAEAEIRK
                     SLIQRGYIKGIIGLPANLFFGTGIPACVIVLDKEGAEERKHIFMVDASKGFIKDGNKN
                     RLREQDIHKIVDVFTKQTDVPKYSRLVSIEEISDPKNDYNLNIPRYIDSQETEDIQDI
                     EAHLLGGIPNADIEALNEYWEVYPSLKAELFNARERENYSDLKIEKDQIKQTIFNHPE
                     FTTFSKKMDEVFETWKTKNTQILKAFVKGFKPKELVFRISEDILATYTGKALMNKYDV
                     YQHLMNYWNDIMQDDCYYLSVEGWKAELTITKQTKSVTEWDCDLVPKSLVIDRYFGVE
                     AQYIATLEAEKETIAIEISELEEEHNTEEGLFADVPDKVNKGNVQKRLKAIDTPKLKT
                     ENAEEIKVLKAYLKLIDDQTAINKKIKEALAELDKKVIERYKTLTEDEIKQLVVDDKW
                     MTVLERDVKTEMERISQRLTQRIKELAERYETTLPQQTTEVAELESKVNTHLQKMGFV
                     WK"
BASE COUNT         2068 a         1506 c         1249 g         2759 t
ORIGIN      
        1 cttcggcaat agcaaagtcg ttttcaagcg gattcttcca gtcaatcagg taaacggttt
       61 ccttgttttc gcccgcttcc tcttttactt ttatgccata acgaagcagt gaataaactt
      121 ctttatttgc ctgataaagg tcgtcgtggg tgtttatatt aactgctttg taaaactcgt
      181 aaagggcttt attgatttgg tttttagtat atcccttttt tgtaagaaaa gcagtaagca
      241 aagcttcatc tacattgctg ttgttttctc gttcttccca gttacccaaa tagcggtatt
      301 ccagttctga ctggaacaaa gcaacaatgc gattttgggt tatgcgttca atttgtccga
      361 ctttactcat gttgtcattc cttcaaaaac attttcaggc aattcaatcc caattttaaa
      421 aaacctgttg tattcttttt cttcttcaag ttgaaaatta tcagctataa aatcctttct
      481 gccggcactt tgactggagt atattacaag acagtctata actgtttttt cttcaatttc
      541 caggaaacta tatatctttt taagtcttgc atagccgctt atttgcctaa tatcatcaac
      601 atttaccttt ccgttttgat attgaggctt gtattttgca tcaacaacca tttttaataa
      661 tccgtcatat gtttttataa taaaatctgg ttccaagtaa ttaaactttt tatgataagt
      721 gacttcttta gacttattaa aacgctcttt taattttgaa aaaatataca attcaaagag
      781 cttactcatg tcaatccaga aaggtggcgt ttttacactg agggagttta cgctcgaaat
      841 attataccca taacgcttta atatatgtcg ggcaaatatc aaagcctgta tatactcttt
      901 gtaaaaattg tttggcttga tattctttaa ttcttcaatg cttactttgc tatctacatt
      961 ttcaaatgca ggttgtatat aattcagcat ttcattcaaa tatgaagtat caataccttt
     1021 aaggtttttc agtgctgaaa tagaaaattc taaggccttc ttaagcactt tattttctat
     1081 gctattgact ccaaactcag aaaactggca acaattataa agcattttat tattgaagtg
     1141 atttttttta atcgtttcat taattaaaat tcttcctttt attttagcat ttagattttt
     1201 tgtaattgga taataggttt ttcttagccc cttttgaaca atttttttaa ggatatttaa
     1261 gtattgaatt attaataaag gagtcaaaat atcttgtcgt tgctctattg ttatcgttgg
     1321 cttattaaag tctatttcac aaagctggtc tagataatta tagttttcag attcttttaa
     1381 gatatcaaaa agcattttta aaaaattgat ttctgttgaa gtatcactca ttttgggctt
     1441 tatatttatt gccagttcat tttctactac ccaatcgata cccacaaaat aagatgcttc
     1501 aaggcgatat ggattttcaa catcgggaaa atggtcaatt ttatagcaat aggttttatt
     1561 atcaccacgc tgaatagatc tacagtacga tttgttattc agtatatctg aatatttatc
     1621 aatgttatca attggtaatg gatttttata accgtattgt tcactaacaa ctatcatgcc
     1681 tataacgctc gtataagggt ttctgcactc tgtttaagaa tcccatcctt taaatactct
     1741 tttaaaatag gaataatttc gtaacttttc cttatagaaa aatcaccttc tcctttaata
     1801 aaataactgt gaccaagcca aacatcctcc ggcctgaatt cttctgatag atgttccgaa
     1861 ggtattaatt ctactgtagt atctagtgca taggcatcaa agttttgtat aaataaagct
     1921 gagacttgtt gaaataattc aagttcaaaa ttctctatat ccagtatttt gggtaataca
     1981 tcaataaaag caaatcgcct gcgtatggcg taatcaatat gtccaacact acggtctgaa
     2041 gtgttcattg tgccgataat gtaaagatta ggtggaatta ttagcgtatt atcatcatca
     2101 acaggataca tactctcgac tttctcacct ctatattcta gagcatagat taattcaccc
     2161 aatacggccg gtaggttggc tctgtttatt tcgtcaatga ttaacacata tttcttttct
     2221 tcaatttcag gtatatctat atcaggtttc ttttttgtat ccatgaacga ataaaacata
     2281 tcaagcactc tcatataata gctggcatgt tgaatcgcca aaccggaaat tccagtcatt
     2341 ttcttgattt cctgtctggt ttttacattg gcacggtaca ttttaatgat atcttcaaaa
     2401 cgcattcggt tacctgcaat gttactccag ttgtctcctg tgtacctaaa gcaatcattt
     2461 tcaacttccc gcaaatatgt tgtagttcct tttagtaata attttccatt tgatttttct
     2521 atttcatctg tgacaaagtc agaaaaatct aaaaaattct cctgaatcca gttttcctct
     2581 tttaattgag ctttatcttt acggctgttt tcataatttc gcaatgctct attagcaaag
     2641 tccaccataa gtttgttttt ggtcttatac tcaatagata tgcctttgct ttccgcaact
     2701 attcctctta caaaatcctc ataggaaaaa gcagggtgaa actggacaaa cttgaattgc
     2761 tctgtgtttt ctagacggtc tttttgaatt gttttatcct ccgaaacttc cgaaaaaagc
     2821 atttgctcgg caatatcttt tgctgtatat gtttttcccg tacctggagg gccttgcaat
     2881 attatctgtc ctttatgtgt taatacatca attatgctct gattgttcat agctgcctca
     2941 ctgaaatgat ttttatcgga aaaaatgatt tggtatgctt caaattgttt tttaaaatct
     3001 gatatcatgc tctcaaaagc atcatcatca atgagaaacc catttctttc gttttttaca
     3061 cttatgcttt ttccaaaaag tgtatattga ttattctgcg gggctttaag ttgctgaata
     3121 tctccataaa caaaagtgta atctggtaat atccctctga cttttttttc attttttaag
     3181 aatccaaaca tataggggcg catataatca acagaactct tttcatttgc aaaaggtaca
     3241 tattgagtat taaagcctaa gccgtatcga atctccaaat catcgacaac aaaggcaatg
     3301 tgatactgta actccgtacc accaccttcg tttattgccc aatcccttga ttggtcagtc
     3361 agtttgaata atattcttct tttggggact ttccctaacc cggccagttc cttccgcttt
     3421 gaaccaaatt catcgcagaa ttctcccaat gttgcattga cttgattaac cagatccgca
     3481 aggtccttaa atttcattac accaacctca ctttaccagt tagcagaacc tgcatcatac
     3541 ccagttttat tttgcggtat ttctccaatt tggtttccaa ggcattgatc tcgttatcca
     3601 tatcggatag gatggtggcg atgcgggttt gttcttcatt tgtttttggt attgacactt
     3661 taaattcaaa taatggattg cgaacaattt gcggttgacc agaaccagta atacattgac
     3721 taaaatcttg ttttgtgaga atgtaataaa agaataactt atcaatattt gtatttttat
     3781 ctacattcat aaccatagca ttacctgtaa tccaagactt tcctaccgtt ctatttacag
     3841 taccacaagt tgatcctctg caagtgattg ttgtctgcca agtttcgtgg ttatataaat
     3901 gatattttcc aacaattcca ttagcaccat aaactaagta tccatcatca ataaaaacat
     3961 cttgtgaaat agtctgcggt tgataaatct ccgctacttc ccccaacttc ttcacttccc
     4021 agccctcttt aggcttaagc aattgttgca ttgccccttg ttttatgttg cgttttttgg
     4081 caatgagttt ttcgaggctg tttatcaggg catcggcatc gcttagggcg gtggctatgg
     4141 cggtttgttc ggatttagtt gggggaagag gaattttcat tttcatcaaa tcaccaatcc
     4201 caacattatc ttgaatgttt gcattaactt tatttttaac ttctaatccg aaaatatttg
     4261 acttgaagta gtgaaataaa aatacgtcat tcagcttcga tttatctggt ttaatagtta
     4321 cacaagagta cgacacaaca aattcttcat caatatccac gtatgacaca cttcctactg
     4381 ttgcataccg agccaaaatt atgtcgcttt ttaaagttct tatttttttt gagagttttt
     4441 caaaatcatc tttgcttatt ttcttgcaat tatcaaaatc tatttcactt gcataatcct
     4501 tgatatcacc tgtcattagg taaggaattc catacttttc ggttttaggc atataatggt
     4561 caacatctgc aattcgaagt aataaatcac ctaaaatata aacttcccaa tcctcaggaa
     4621 ttattccgac ttcagtaact ttataaccct ttggcaattg ttcattatcc atcatttaat
     4681 cctttttaaa agttcattaa ccaaagaatc cattcgcgaa aaggcaaacc attttttgcc
     4741 caaatctttg agcgaggcgc ctatatggta gagttccttg tggtcgataa ttaaaaacct
     4801 gtcgtggcta ttggcgaatg tcttaatctc aatgcttgga tactgtgcat tgtgcttttt
     4861 tacatccaat tgcaatgctt tattaatctc ttttgtataa atgcttgcag atactcctat
     4921 atttcgcttg ctcaacagcg taagaaccga ttcatcaatg taattatcaa taagaacaat
     4981 gctttgggtt gctgatttta tcaagttgac agcaaaggta taggcatcaa acacctgccc
     5041 gtcgaagaaa attcccatat caggcgtggc ttgtccgctt tccagtgctt tgaatattct
     5101 ttcaaatttc tcatccgttt gaatttgttt caattcaatg cggtcaagtc gttgaaatac
     5161 agaagcattt tgaaataaaa acttacgcat ggccacaaaa gcccttgcaa tggtaatgct
     5221 cacctctgct gctttttcgc ttttaagcac accggcaaca gcagctaccc cctgttcggt
     5281 aaatacatac ggaagatgtc cgcccaggct ttgtttagaa ggtatcgcat tttgcgatac
     5341 caaactatca acctcgtttt ccgttaattg aaatacaaaa tctggaggaa atcgatttgg
     5401 gtttctttta acttgctccc ttagccttat aggttttact tcataaaatg aagctaaatc
     5461 tttgtccaat attacctgca catcacgtag catcactatt tttgactcaa tatgctgctg
     5521 cttaactatt tccatacaaa ccccattttt tgtaggtgag tattcacttt gctttctagt
     5581 tcggcaactt ctgtggtttg ctgtggcaac gttgtttcgt agcgttctgc aagttccttt
     5641 atacgctggg tgagtcgttg gctaatgcgt tccatttcgg tttttacatc acgctccaaa
     5701 acggtcatcc atttgtcgtc taccaccagt tgctttattt cgtcctcggt aagggtctta
     5761 tagcgttcaa ttaccttttt atcgagttcg gcaagggctt cttttatttt tttgttaata
     5821 gcggtttgat catcaatcag ttttaaatat gctttcaata ctttaatttc ttcggcattt
     5881 tcagttttca attttggtgt atcaattgct ttcaatcgct tttgcacatt ccctttgttt
     5941 accttatcgg gaacatcggc gaataaccct tcttcggtat tatgttcttc ttccagttcg
     6001 gaaatttcta ttgcaatggt ttctttttcg gcttcgagag tcgcaatgta ttgcgcctct
     6061 acgccgaaat agcggtcaat taccaaagat tttggtacca ggtcgcaatc ccattcggtt
     6121 accgatttgg tttgcttggt aatggtcagt tctgctttcc agccttcaac cgaaaggtag
     6181 tagcaatcgt cctgcatgat gtcgttccag tagttcatca ggtgctggta cacatcgtat
     6241 ttgttcatca gggctttgcc tgtgtaggtt gccagaatat cttccgaaat tctaaaaaca
     6301 agttcttttg gtttaaagcc ttttacgaag gctttcagga tttgggtgtt tttggttttc
     6361 catgtttcaa atacctcgtc cattttttta gagaatgtgg taaattcggg atggttgaaa
     6421 atggtttgtt ttatctgatc tttttcaatt tttaaatcgc tgtaattttc acgctctctt
     6481 gcattgaaaa gttccgcttt cagtgaaggg tacacttccc aatattcgtt taacgcttca
     6541 atgtcggcat ttggtattcc acccaataaa tgagcttcaa tatcctgaat atcttcggtt
     6601 tcctgactgt caatatatcg gggaatgttc aggttgtaat cgttttttgg gtcgcttatt
     6661 tcttcaatgc ttaccaaacg actgtatttt ggcacatcgg tttgtttggt aaaaacatct
     6721 acaattttat gaatatcctg ctcacgcaaa cggtttttgt tgccgtcttt tataaagcct
     6781 ttgctggcat caaccatgaa aatgtgtttg cgttcctcgg caccttcctt gtcgagcaca
     6841 atcacacagg caggaatacc cgtgccgaaa aagaggtttg cagggagtcc gataattcct
     6901 ttgatatatc cacgctgaat taagctttta cggatttcgg cttcggcatt tccacgaaac
     6961 aaaacgccat gaggcaaaat acaagcccct ttgcctgtgc ttttaagcga tttaagtata
     7021 tgcagcaaaa aagcataatc gccgtttttt tcgggcggcg ttttataaat ggttttacct
     7081 gtttttgcac tttcttcttc gtctttttca aagcgtccgt atatatcatt ctgcgggtcg
     7141 aaaccattta cccagttttt tgatgaaaaa ggcggattgg ctaccacata atcgaaggtt
     7201 ctgagataac cgtctttgtt tttaaaaaat gggtctgaaa gggtactttg accacggtgt
     7261 atttcgtgtc cctcaatacc gtgcagaatc atgttcatac gagccaaagc agccgttgcc
     7321 acatcttttt cctgtccgta gatagaacct ttgccatcgg cttcgtcaat cacttttaaa
     7381 agcagtgaac ccgaaccaca agtggggtca taaaaggttg ttgagttttt agggtcggta
     7441 attcctaaaa cttttgccaa tacacggctt acttcacttg gagtataaaa ctgcccttta
     7501 ctcttaccac tttcggtggc aaagttcttc atcaggtatt caaaggcatc gcccagaata
     7561 tcatcgccac cggcacggtt tt
//
LOCUS       DAOSPF010000114         7563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_9717_length_7563_cov_6.118673, whole genome shotgun sequence.
ACCESSION   DAOSPF010000114 DAOSPF010000000
VERSION     DAOSPF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_7563_cov_6.118673"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1142)
                     /gene="ppk2"
                     /locus_tag="PK252_14070"
     CDS             complement(312..1142)
                     /gene="ppk2"
                     /locus_tag="PK252_14070"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOT15879.1"
                     /translation="MSKEKENKEKNHKEEKEQHKQEKKEYEKNLVHLQLELVKLLEWI
                     KFKKLKVVVIFEGRDAAGKGGVIKRITEYLNPRFCKVVALGVPTEREKTQWYFQRYVA
                     HLPAAGEMVLFDRSWYNRAGVEHVMGFCTDEEYEEFLRSCPEFERMLIRSGIILIKYW
                     FSVSDEEQERRFQDRINNPAKRWKISPMDLESRKHWVEYSKAKDTMFQYTDIKQAPWY
                     VVNADNKLKARLNCINHLLSMIPYEEIKQEKLKLPPRQSDEGYIRPPVTDQTFVPEKY
                     "
     gene            1425..2711
                     /locus_tag="PK252_14075"
     CDS             1425..2711
                     /locus_tag="PK252_14075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT15880.1"
                     /translation="MATFDIKMPKLGESVEEATITKWFVKAGDMVEEDQVLLEIATDK
                     VDSEIPSPVSGKVIEIKYPENAKIAVGKVIAVIDTDGSAEVKPSASIAVEEVKAEEPV
                     KTNVVVNESLNFNESSRFYSPLVRSMAKKEQVSLDELEKINGTGHNGRVKKEDLLHYL
                     ENRTSGSAKPVTESVVAKAESKPVEKPLVISSSEDEIIEMGRMRRIIADHMVMSKQVS
                     PHVTNVVEADVTSLVMWRNKNKDAFEKKQGVKLTYMPVFIEAVARALKEFPMVNASVD
                     GYKIILRKNVNVGVAVALPNGGLIVPVIRNADLKNIAGLAVDMNELAEKARKNKLSPD
                     DIQGGTFTITNFGTFRNLYGTPIINQPQVAILATGSIVKKPAVLETPQGDVIAIRHKM
                     YLALTFDHRIIDGVLGGTFLRRIADILEQWDESTSI"
     gene            2794..4830
                     /locus_tag="PK252_14080"
     CDS             2794..4830
                     /locus_tag="PK252_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT15881.1"
                     /translation="MPRVHGILTTDKELLEKWFYLMVLGRALDDRAPNYLKQAIGWSY
                     HAPYAGHDGIQLAIGQVFDKTTDHLYPYYRDMLTTLSAGLTAEEIILNGISKDNDVAS
                     GGRHMSNHFAKPEWNIHNFSSATGNQTLHATGTGRAMKKYNHKGVVFCSQGESSVSEG
                     YVFEAINGASNEKVPVVFIFQDNGYGISVPKKDQTANRKVSDNFRGLKNISIIKCNGK
                     DVFDSMNAMLEAKRIALHEQHPVIVHANCVRIHSHSNSDRHELYRDDYELNYVREYDP
                     LAKYRRLLLRYKRFTEEELVAIEQRVKDTVKEAHKSAMASPDPKPESIHDFVIGPAYV
                     SQKYPEGLHDGKGVEMKLIEAINQTLKAEFRHNPDTFLWGQDIANKDKGGIFNVSKGM
                     QQEFGAERVFNAPIAEDFILGTANGMSRFNKNIRIVVEGAEFADYFWPAMEQLINTSH
                     DYWRSKGQFSPNITVRLASGGYIGGGLYHSQNIEGALITIPGIRVVYPSFADDAAGLL
                     RTSMRSEGLTMFLEPKALYNAPKAATLVPEDFEVPFGKARIRREGKDMSIITYGNTTH
                     MCIEVANQVSSELGKEIEVLDLRSLSPLDHEAIVNTVKKTNKVLIVHEDKVIGGVGGE
                     VAALISNEVFEYLDAPIMRVGSTFTPVGFNRILEKAILPNNEKILEAVKKMLNY"
     gene            4887..5396
                     /locus_tag="PK252_14085"
     CDS             4887..5396
                     /locus_tag="PK252_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOT15882.1"
                     /translation="MEKIALIYSYNTRNTANVAKEIVKAFGKSVVVEELNAEDIDGQK
                     FSSYNNLILGVPTWFDGELPNYWDEFVPELETLDLKGKRIAIFGLGDQVNYPENFVDG
                     IGLMVNICKKAGAEIVGYTDASGYTFESSAAFSGGKFCGLAIDIENQGDLNDERIESW
                     VSQLKKEFR"
     gene            complement(5405..6532)
                     /locus_tag="PK252_14090"
     CDS             complement(5405..6532)
                     /locus_tag="PK252_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT15883.1"
                     /translation="MKIAVNTRLLLHDKLSGIGWFKYHVLKRMVEQHPNDEFVFLFDR
                     QYDERFIFGPNVIPEVVFPQARHPFLYYIWFNYSIVPVINKHKADVFLSPDGLLSTRL
                     PFIPSVPVIHDINFQHYPKDIPYFMRHYYCHYFPIYAKIAKRIITVSEFSKKDIVKTY
                     GADADLIDVAYNGAGEDFKPLDEYQKHEVRNQFANGAEYFVYVGNLLPRKNVDGMIKA
                     FTLCKNKTGLSTKLVIVGESFFMTDKIKEAYDNSNVKDDIIFTGRLTQEELPLVVGAA
                     KGLIFVSHFEGFGIPILEAMQCDVPVITSNTSSMPEVAGDAAILADPNNIGQIAQAIE
                     DIDTKNNLRNELIEKSRIRRQAFNWDKTAEEVWKSLTKAVF"
     gene            complement(6529..6768)
                     /locus_tag="PK252_14095"
     CDS             complement(6529..6768)
                     /locus_tag="PK252_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT15884.1"
                     /translation="MKNRVKAEREFKEMTQDQLAQLVGVSRQTIHAIEHDRFIPSTLL
                     ALKLSKVFKRKVNDLFYLEEFDWEIPVQTNTDKKK"
     gene            complement(6874..7014)
                     /locus_tag="PK252_14100"
     CDS             complement(6874..7014)
                     /locus_tag="PK252_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15885.1"
                     /translation="MSFECPYYNDNYCRLQQGKCQPAIGKCILKGKVKRAKDEDNEPI
                     SK"
     gene            complement(7126..>7563)
                     /locus_tag="PK252_14105"
     CDS             complement(7126..>7563)
                     /locus_tag="PK252_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT15886.1"
                     /translation="VDSSAKAIALTNENIALNNFNTLNHEAIVADGLEYMREIKDKYN
                     LIILDPPAFAKHNAALDNALQAYKRINQKAIEQIRGGGVLFTFSCSQVVTKELFRQAI
                     FVAAANAGRSVRILHQLTQPADHPVSIFHPEGEYLKGLVVYIE"
BASE COUNT         2233 a         1420 c         1546 g         2364 t
ORIGIN      
        1 tgtttgaaaa attttattaa actattgaat tacaattttt taatcgatta aacattttta
       61 atgcttaatt tgaaattatt ggatttatat gctgttttta ttcaacgtaa aagttttgct
      121 gaaaaaaata gcagaataga tctttgaatt atctattgat gtgtgctgtt tttttgttgt
      181 tcctttaaaa attttaaatt tatacacgtg aaaaacaaaa gttacccact aattcatgta
      241 caaaactcaa aaaaagatat cttcgcattt tattaattgt tgataattag caagttggtt
      301 tttttaaagt tttaatactt ttctggaaca aaggtttggt cagtaaccgg aggtctgata
      361 taaccttcat cactttgtcg gggggggagt ttaagtttct cctgcttgat ttcttcgtaa
      421 ggtatcatgc ttaataagtg gttgatgcag ttgagccttg cttttagttt attatcggca
      481 tttactacat accatggggc ttgcttgatg tctgtatatt gaaacattgt atcttttgcc
      541 ttggaatatt ctacccaatg cttgcgtgat tcaagatcca tagggcttat cttccatcgc
      601 tttgccggat tgtttatcct gtcctggaaa cggcgttctt gctcttcatc gcttactgaa
      661 aaccagtatt ttatgagtat aattcctgaa cggataagca tacgctcaaa ttcaggacac
      721 gaacgtaaaa attcttcgta ttcttcatca gtacaaaacc ccattacatg ttcaaccccg
      781 gcacggttgt accagctgcg gtcaaataac accatctcgc cagcagctgg caaatgagct
      841 acataacgtt gaaagtacca ttgtgttttt tcgcgttcgg taggcactcc caacgctacc
      901 actttgcaaa aacggggatt taggtattcg gttatacgct tgataactcc gcccttacct
      961 gcagcatcac ggccttcgaa aataacaaca acttttagct ttttgaactt aatccattct
     1021 aaaagcttta ctagttctaa ctgaagatga accaaatttt tctcatattc ctttttttct
     1081 tgtttatgtt gttctttttc ttccttatgg ttcttttcct tgttttcttt ttcttttgac
     1141 atagcggtaa tatttaatta gtaaattagg gaattagcca atttgttctt taaatctgtc
     1201 ttagaaatct taactaaaga tagtcaattt acaatcgaaa taactttcag agaggcaaat
     1261 tattagcatt atattttaaa aaataatgat tgaaaacgat gtaatgccaa gcttaatatt
     1321 tcaaattttt gatttgttaa ctatttcaca atcaaaataa taatacttgt cattgaacaa
     1381 aaaataatcc ttgtggttaa atctataaaa ccgaaaatag aattatggca acatttgata
     1441 taaaaatgcc caagttgggc gaaagtgtag aagaggcaac cattacaaaa tggtttgtaa
     1501 aagctggtga catggtagaa gaagaccaag ttcttcttga aatagctacg gataaagtag
     1561 attcagagat accaagtcct gtatcgggca aggtgattga aattaagtat cccgaaaatg
     1621 ctaaaatagc cgtaggtaaa gtcattgctg ttattgatac tgatggaagt gctgaagtaa
     1681 agccttctgc atcaattgcc gttgaagaag tcaaggctga agaacctgta aaaactaatg
     1741 ttgttgtaaa cgaaagccta aactttaacg aatcttcaag gttttattcg ccattggtcc
     1801 gaagcatggc taaaaaagaa caagtaagtt tggatgaatt agaaaaaatc aatggaacag
     1861 ggcataatgg cagggtaaag aaagaagacc ttttacatta tttggaaaac cgtacttctg
     1921 gttcagcaaa gcctgtaaca gaaagtgttg ttgccaaagc agaaagtaaa ccagttgaaa
     1981 aacctcttgt tatatcatct tctgaagatg aaattattga aatgggccgt atgcgccgca
     2041 ttattgcaga tcacatggtg atgtccaagc aggtttcacc tcacgttacc aatgttgttg
     2101 aagcggatgt tacgtcattg gtgatgtggc gcaataagaa caaggacgca tttgagaaaa
     2161 agcagggcgt taagcttact tatatgcctg tatttattga agctgtagca agggcattga
     2221 aagaatttcc gatggtcaat gcttctgtgg acgggtataa aattattctt aggaaaaacg
     2281 ttaatgtagg cgttgccgtt gctcttccta atggggggct tattgttcca gtcattagaa
     2341 atgcagacct caaaaacatt gccggattag ctgttgatat gaatgaactt gccgaaaaag
     2401 ccaggaaaaa caagctttcg ccggatgata ttcagggcgg gacttttact attaccaact
     2461 ttggtacatt ccgcaacttg tacggaacgc caattatcaa ccagcctcag gtagcaatac
     2521 ttgcaacagg ttctattgtc aaaaagcctg ctgtgcttga aactcctcaa ggcgatgtaa
     2581 ttgctatacg tcataaaatg taccttgctt taacttttga ccatcggatt attgacggtg
     2641 tgcttggtgg tactttcttg agacgtattg ctgatatcct tgaacaatgg gacgaatcaa
     2701 cttctattta atctgaatta gtaataatta ctcaatacac aacaaatcat ggctacaatt
     2761 tttgaaaaga tattggaaga acaaaacaaa gaggtgcccc gggtacacgg tattcttacc
     2821 actgacaaag agctgctcga aaagtggttt tatcttatgg tacttggacg tgcattggac
     2881 gatagggccc cgaattacct gaagcaggca atagggtgga gctaccatgc cccttatgcc
     2941 gggcatgatg gaatacagtt ggctattgga caggtttttg acaaaactac agaccatttg
     3001 tacccatatt accgggatat gttgacaacc ttgtcggcag gccttactgc tgaagagatt
     3061 attcttaatg gtatttcaaa agataatgat gtggcaagtg gtggaaggca catgagcaac
     3121 cactttgcaa agcctgaatg gaacattcac aacttttcat cggccacggg caaccagacg
     3181 ttgcatgcga caggtaccgg aagggccatg aaaaaatata accacaaagg tgtggtgttt
     3241 tgctctcaag gcgaatcttc tgtttcagag ggttatgtgt ttgaagccat taacggtgct
     3301 tcaaatgaaa aggtgcctgt cgtttttatc tttcaggaca atggttatgg aatatcggtg
     3361 cctaaaaaag accagactgc caatcgcaag gtttccgata attttagggg tttaaaaaat
     3421 atttctatca taaaatgcaa tggcaaagat gtctttgatt ctatgaatgc tatgcttgag
     3481 gctaagcgca tagctttgca tgaacagcat cctgtcattg tccatgccaa ctgtgtccgc
     3541 atccattcgc attccaacag cgaccgtcac gagctgtacc gagatgatta cgagttgaat
     3601 tatgtccgcg aatacgaccc tctggctaag tacagacgtt tattactccg atataaaagg
     3661 tttaccgaag aggaattggt ggcaatcgag caaagggtaa aggatacagt taaagaagct
     3721 cacaaatcag ccatggcttc gcctgatccc aaacctgagt ctattcacga ctttgtcatt
     3781 ggcccggctt atgtgagcca gaaatacccc gaggggttac acgatgggaa aggtgtagaa
     3841 atgaaactga ttgaggccat caaccaaaca cttaaggccg aatttaggca taaccctgat
     3901 actttccttt ggggacagga tatcgccaac aaggacaaag gcggtatttt caatgtttct
     3961 aaagggatgc aacaggagtt tggtgctgaa agggttttca atgcccctat tgctgaggat
     4021 ttcattctgg ggaccgccaa tggcatgtcc cgattcaata aaaatatcag gatagtggtt
     4081 gaaggtgccg aatttgcaga ctatttctgg cctgccatgg aacaacttat caatacatcc
     4141 catgattact ggcgttcaaa aggtcagttt tcacccaata taacggtacg tttggcttct
     4201 ggtggctaca tcggtggagg tctttaccat tcgcaaaaca ttgaaggggc tttgattacc
     4261 attcctggca ttcgagtggt ttatccttcg tttgccgatg atgctgctgg cttgctgcgt
     4321 acctctatgc gttcggaagg attgacgatg tttttagaac ctaaggcatt gtacaatgcc
     4381 ccaaaagcag ccactctggt tcctgaagat tttgaagtcc cttttggtaa ggctcgtata
     4441 aggcgtgagg gaaaggatat gtctatcatt acttatggta acaccacgca catgtgtata
     4501 gaagtggcta accaagtgtc atctgaattg ggcaaagaga tagaagttct ggatttgcgc
     4561 tctctcagtc ctctggatca tgaagctatt gttaatactg tgaaaaagac caacaaagtg
     4621 ctgattgtac atgaggataa agttattggt ggagtagggg gcgaggttgc agcattgata
     4681 agcaatgaag tattcgaata tcttgatgcc ccaatcatgc gggtaggttc aacatttacc
     4741 cctgtcgggt tcaaccgtat tcttgaaaaa gctatcctgc caaataatga aaagattttg
     4801 gaagcagtga aaaaaatgct caattactaa tgatgaatat ggtatttctt ttgccttttt
     4861 acttataact ttttacttta tctaatatgg aaaaaatagc acttatatac agttacaata
     4921 cccgtaacac agccaatgtg gctaaagaaa ttgtcaaagc ttttggaaaa tctgttgttg
     4981 tagaagagct taatgccgaa gatattgatg gacaaaagtt ttcatcttat aacaatctta
     5041 ttttgggcgt tccaacctgg tttgatggcg aattgcccaa ttattgggat gagtttgttc
     5101 cagagctcga aacgcttgat ttgaaaggga aacgaattgc catctttggc ttgggtgacc
     5161 aggtgaatta tcctgagaac tttgtggacg gcattggcct catggtcaat atttgtaaaa
     5221 aagccggggc tgaaattgtt ggttacacag atgcttcagg ttataccttt gaatcttctg
     5281 cagcattttc aggtggaaag ttttgcggat tagctataga tatcgaaaat caaggcgatt
     5341 tgaacgatga acgcattgag agctgggttt cacaattgaa aaaggaattt aggtagtttt
     5401 acctctaaaa caccgctttt gtcaagcttt tccaaacctc ttcagcggtt ttgtcccagt
     5461 tgaacgcttg tctgcgaatg cgggactttt ctatcagctc gtttctgaga ttatttttgg
     5521 tgtcaatatc ttcaatggct tgtgctatct gtccaatatt gtttgggtca gccaaaatag
     5581 ctgcatctcc tgctacttca ggcattgatg aggtatttga ggtaattacc gggacatcgc
     5641 attgcatagc ttccaaaatg gggataccaa agccctcaaa atgagacaca aatatcaagc
     5701 ctttggcagc tccgactacc aaaggtagtt cctcctgtgt cagtcgtccg gtaaagatta
     5761 tatcatcttt aacattggaa ttgtcatacg cttctttgat tttatcggtc ataaaaaagc
     5821 tttcgcccac tatcaccagc tttgtgctca aacccgtttt atttttgcac aaagtaaaag
     5881 ctttaatcat gccatcaacg ttctttctgg gaagcaggtt gcctacatac acaaagtatt
     5941 ctgccccatt ggcaaattga tttcttactt catgtttctg atattcatca agcggtttga
     6001 aatcttctcc tgctccattg taagctacat caatcaaatc agcatctgct ccgtatgttt
     6061 tcactatatc cttctttgaa aattctgata cggtaatgat gcgtttggct attttcgcat
     6121 agattggaaa gtaatgacaa taataatggc gcatgaaata ggggatatct ttcgggtaat
     6181 gctgaaaatt gatatcgtgt atcactggta ctgacgggat aaacggcaaa cgtgttgaca
     6241 acaatccgtc tggcgaaagg aaaacatcag ccttatgttt gttaataaca ggcactatcg
     6301 agtaattgaa ccatatatag tatagaaacg ggtgcctggc ctggggaaaa actacttcag
     6361 ggatgacatt tggcccaaaa ataaaacgtt cgtcgtattg ccggtcaaac aaaaaaacaa
     6421 actcatcgtt agggtgttgc tcgaccattc gctttaacac atgatatttg aaccagccaa
     6481 tgccgctcag tttatcatgc aacaacaaac gcgtatttac agctattttc atttcttttt
     6541 gtcagtgttt gtttgaacgg gtatttccca gtcaaattct tcgagataaa ataaatcgtt
     6601 tacttttcgt ttgaaaacct ttgaaagctt taaagccaaa agggtagaag gtataaagcg
     6661 gtcatgttct atagcatgaa tggtttgtcg gctcacgcca acaagctgag caagctggtc
     6721 ttgcgtcatt tctttaaact ctctttcagc ttttaccctg tttttcatgc cataaaatta
     6781 attagcaaat ctaaaaatta gcaaatttga aaatgaaatc ctactcaaga cttttagtta
     6841 ttcaaatata tatcttttca ttatcaaatt atctcatttg ctaattggct cattgtcttc
     6901 gtcttttgcc cgttttactt tgcctttcag gatgcatttg ccaattgccg gctggcattt
     6961 tccttgctgc aacctgcaat aattgtcgtt gtaatatgga cactcgaagc tcatcttttt
     7021 aatttgaaaa tttgagaatt agctaatttg aaaatgaaat cttactccaa aatgcatagt
     7081 tatttatgtt ttatcattag ctcattatta cattcgctaa tttactcatt caatatatac
     7141 aacaagccct ttcaaatatt ccccttccgg gtgaaagatg cttacagggt ggtctgccgg
     7201 ctgggtcaac tggtgcaata tacgaaccga acgcccggca ttggctgccg caacaaatat
     7261 tgcctgcctg aacagctctt tggtaaccac ttgcgagcag gagaatgtaa ataaaacacc
     7321 gccaccgcgt atttgttcaa tggctttctg gttaatcctt ttatacgcct gaagtgcatt
     7381 gtcaagtgct gcattatgct tggcaaaagc aggagggtcg aggataatca ggttgtattt
     7441 gtcctttatt tcacgcatat attccaaacc atctgccaca atggcttcat gattaagtgt
     7501 gttaaaatta tttagggcaa tgttttcatt ggtaagggca atagctttgg ccgaactgtc
     7561 aac
//
LOCUS       DAOSPF010000115         7430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_9982_length_7430_cov_8.660339, whole genome shotgun sequence.
ACCESSION   DAOSPF010000115 DAOSPF010000000
VERSION     DAOSPF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_7430_cov_8.660339"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1691)
                     /gene="groL"
                     /locus_tag="PK252_14110"
     CDS             complement(51..1691)
                     /gene="groL"
                     /locus_tag="PK252_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT15887.1"
                     /translation="MAKEIIFDLEARQALLRGVDALANAVKVTLGPKGRNVVIDKKFG
                     HPHVTKDGVTVAKEIELSNHIENIGAQLVKEVASKTNDDAGDGTTTATVLAQSIVTVG
                     LKNVTAGANPMDLKRGIDKAVATVVESLKKQSKVIGEDNQKIEQVATISANNDSSIGK
                     LIAEAMAKVKKEGVITVEEAKGTDTEVKVVEGMQFDRGYISPYFVTDTEKMEAVLENP
                     YILIYDKKISTMKDMLPILEAVVQTGRPLLIIAEDVDGEALATLVVNKLRGSLRIAAV
                     KAPGFGDRRKEMLEDIAVLTGGVVISEEKGMRLDSVGINDLGKAEKITIDKDNTTVVN
                     GAGDKKNIDARVAQIKAQIENTTSDYDREKLQERLAKLAGGVAVLYIGAATEVEMKEK
                     KDRVNDALSATRAAVEEGIIPGGGVGFIRSIASLDNLKGDNDDETTGIAIVKRALEEP
                     LRMIAVNAGQDGAVIAQKVKEGKDDFGYNARLEKYENLFAAGVIDPTKVARVALENAS
                     SIAGMILTTDCLVVEKPEDKKDAGMGGAPGMGGMGGMM"
     gene            complement(1746..2015)
                     /locus_tag="PK252_14115"
     CDS             complement(1746..2015)
                     /locus_tag="PK252_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT15888.1"
                     /translation="MKIKPLADRVLVEPMAAEQKTASGIIIPDTAKEKPQRGKVVAVG
                     KGAKDEPMELKVGDEVLYGKYAGTEISIEGKDYLMMRQSDVLAVI"
     gene            complement(2231..2578)
                     /gene="secG"
                     /locus_tag="PK252_14120"
     CDS             complement(2231..2578)
                     /gene="secG"
                     /locus_tag="PK252_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT15889.1"
                     /translation="MTIAYIFIFIVCVLLILIVLLQNSKGGGLAANFTGSQVMGVRKT
                     ADFLEKATWVLAGTLLLFCVIINIVMTRHTETVEKSMLQDEVKNAINPNAVPNIPKSA
                     PAQMPQQQNEEKK"
     gene            complement(2590..3582)
                     /locus_tag="PK252_14125"
     CDS             complement(2590..3582)
                     /locus_tag="PK252_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15890.1"
                     /translation="MELNKEDILSLLNNVKALEAVDVESLNCILNQYPYFQPIYFLLL
                     RYYKEKDEAEYANLLKKAVVHVSNRKHLFKYINLSHAKTILTEKPVENSGIEPAEPSI
                     RKEEKEFLSDAIADAVSQQISGKTETGFEQRLLPEITFELDESIEIIRPKDVATVDFA
                     IENDILDTERIDQSILIIEDEQIKQDVRGVGFEFFEKKTDNSALRFSPEKANNKNDLS
                     ENISLDIGGPEDGVTGQSEITGPEPDLIDKFLADMPKIRPKQVEEFVEYDISTNSVEE
                     HDDFMTETLAKIFVRQKNYQKAIDTYSKLSLKFPEKSTYFANQIFEIKKLMNNQ"
     gene            complement(3586..4119)
                     /locus_tag="PK252_14130"
     CDS             complement(3586..4119)
                     /locus_tag="PK252_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOT15891.1"
                     /translation="MRPFEKKYRFLLIVFAAIMLVVQSCFTVKYSTSGASISPDVKTV
                     SISTFQNRGGTVRATMAYDLTNKLREKIEQNTKLYFVTDGGDVSFEGEITGYTVETQA
                     LQSSDKVTKERLKVTVNVKFVNTVEPQYNFSSSFSRYEDYDVSSGESLESAENRLFPI
                     ILDNLAEDIFNKAFTNW"
     gene            complement(4116..5339)
                     /locus_tag="PK252_14135"
     CDS             complement(4116..5339)
                     /locus_tag="PK252_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT15892.1"
                     /translation="MDVTSIKQRFGIIGNNNALNRAIDIARQVAPTDLSVLITGPSGA
                     GKEVFPQIIHSFSARKHGQYIAVNCGAIPEGTIDSELFGHEKGSFTGAHESRKGYFEV
                     ANGGTIFLDEVAELPLSTQVRLLRVLETGEFLKVGSSKVQKTNVRVVAATNVDIPKAI
                     DNGKFREDLYYRLNTVPIFVPPLNQRAEDIHLLFKKFAADFAEKYRMPAISLEPEAVK
                     VLETYTWPGNVRQLKNIAEQISIIEKKRVIDETTLRLYLPDYQSERLPMLFKRNEPDS
                     SFASEREILYKILFDMKADLHELKKTVFELTHNRPFNEEHPFDNSKDKSSVFKNLDFD
                     TNSFVKTYDIKKTSPVIEETQEVIEETLSLQEKEKELIKKALEKHGGKRKLAADELGI
                     SERTLYRKIKEYNME"
     gene            complement(5481..5696)
                     /locus_tag="PK252_14140"
     CDS             complement(5481..5696)
                     /locus_tag="PK252_14140"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOT15893.1"
                     /translation="MKKFFLLLFIFFLAYTLIKAILRLIHIFRSDNHKKSKGDYHYFD
                     NTTVENNSKKKRFNKDDGDYVDFEEVK"
     gene            5868..7079
                     /locus_tag="PK252_14145"
     CDS             5868..7079
                     /locus_tag="PK252_14145"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT15894.1"
                     /translation="MSEFLKKRGTIAILTGGGDVPGLNPAIRAITLRAIEEGYQVIGL
                     KRGWLGITELIRDKNADNSNNYMVLSEEIVNKSGRTGGSFLHTSRTRPSHFAKDLVPV
                     HLQSKYNKEINDLTPEVLKNLDYLGVDYIIPIGGDETLSYAVRMCQEGVKVIAIPKTM
                     DNDVPGTDYCIGFSTCITRTISMVNTLRTTAGSHERILVVEIFGRYAGFTAMLPTMAG
                     AASRCVIPEHKFNIEKLTEIMVHDRNTNPSKYSVLLVSEGATMEGGDEKVMPTKESEM
                     HLNESIGSIGDVVSRKIRELSPKYNNGKTINVIEQNLGYLVRGGDPDAIDAIVPMAFG
                     NLALDLILKGIHGRLVVLRNGRYDNAPLDVITSQRKTVDVKRFYNTERYRPHYKSFEF
                     QPLFIMTGNLS"
BASE COUNT         2158 a         1516 c         1308 g         2448 t
ORIGIN      
        1 aaaaaaggca gcctcacagc tgcctttata tttgggattg atgaattatc ttacatcatg
       61 ccgcccatac cgcccattcc cggagcgcca cccataccag cgtctttctt atcttctggt
      121 ttttctacaa ccaaacagtc agttgtaaga atcataccag cgatagaaga agcgttttcc
      181 aacgctacac gggccacttt agttgggtcg attacacctg cagcaaaaag gttttcgtat
      241 ttctcaagac gagcattata accgaagtca tcttttcctt cttttacttt ttgagctata
      301 acagcgccat cctggccagc gttaacagca atcatacgga gaggctcttc caatgcacgt
      361 tttacgatag cgatacctgt tgtttcgtca tcattgtcac ctttgaggtt gtctaatgaa
      421 gctatagagc ggatgaagcc aacaccacca ccagggatga taccttcttc aacagcagct
      481 cgtgttgcac taagagcgtc gtttacacgg tctttctttt ctttcatttc aacttcggta
      541 gcggcaccaa tgtaaagaac tgcaacacca ccagctaatt ttgccaaacg ttcctgaagt
      601 ttttcgcggt cgtagtcgct ggttgtattt tcaatctgag ctttgatctg agctacacgg
      661 gcatcaatgt ttttcttgtc accagcaccg ttaacaacag tagtgttgtc tttgtcgata
      721 gtaatttttt cagctttacc aaggtcgttg attccaacag agtcaaggcg catacctttt
      781 tcttcgctga taacaacacc gccagtaagg actgcgatat cttcaagcat ttctttacga
      841 cgatcaccga aacctggagc tttaacagct gcaatacgca atgaaccgcg gagtttgtta
      901 acaactaatg ttgctaatgc ttcgccatca acatcttcag caataattaa aaggggacgg
      961 ccggtttgaa caacagcttc gaggatagga agcatgtctt tcatggtcga aatcttctta
     1021 tcataaataa gaatataagg attttcgaga acagcctcca ttttctcggt atcagttacg
     1081 aaataaggag aaatataacc acggtcgaat tgcatacctt caacaacttt cacttctgtg
     1141 tcagtccctt tggcttcttc aacagtgata acgccttctt tcttcacttt tgccattgct
     1201 tcggcaatca gtttacctat tgagctgtca ttattagccg aaatagtagc cacttgctct
     1261 attttctggt tgtcttcgcc aataacctta ctttgttttt tgaggctttc aacaacagta
     1321 gcaacagctt tgtctatacc acgtttcaag tccatagggt ttgctccggc tgtaacgttt
     1381 ttcaaaccca cagtaacaat tgactgagcc aatactgtag cagtagttgt tccatctccg
     1441 gcatcatcgt tggtttttga agcaacttct tttactaatt gggctccaat gttttcgata
     1501 tggtttgaaa gttcaatttc ttttgctact gtaacaccat ctttggttac gtgtgggtgc
     1561 ccaaattttt tgtcaattac tacattacgg ccttttggcc caagtgtaac ttttactgca
     1621 tttgcaagtg catctacacc tctaagcagc gcctgacggg cttccagatc aaaaatgatt
     1681 tcttttgcca tgatataatt atgttttaga ttgttatttt atgatttgtt attataaaat
     1741 gtcttttaga ttactgctaa tacatcgctt tgacgcatca ttaagtaatc tttaccttca
     1801 atgcttattt ctgtacctgc atatttccca tataatactt cgtctccaac tttcaattcc
     1861 ataggctcgt ctttagcgcc tttacctaca gctactactt taccacgttg tggtttttct
     1921 tttgctgtgt caggaatgat aattccactt gcggtttttt gttcggcagc cattggctca
     1981 accaatactc tgtcagctaa aggtttaatt ttcaattcag acataactta tcttattaat
     2041 ggttaaacaa tatttaaatt ttgacttttg ataaatcaca aattgtgcca caacgctttt
     2101 ttatcaaaaa aggcaaaata aactgtcaaa atttcagatt ttaaaaaaaa agacagaatt
     2161 aatctgacaa tattttcaat tttaaactaa gtaataaaaa aaagacgggc tcaaaacccg
     2221 tcttctatta ttatttcttt tcttcgttct gctgctgtgg catttgtgca ggcgccgatt
     2281 tgggaatgtt tggcactgca ttggggttta tggcattttt cacctcatcc tgtaacattg
     2341 atttttctac agtttctgta tgccttgtca tcacaatatt gataattaca caaaataaaa
     2401 gcaacgtccc ggcaagaacc catgttgctt tttcaagaaa atcagctgtt ttacgaactc
     2461 ccatcacctg gcttcctgta aaattagctg caagtccgcc tccttttgaa ttttggagca
     2521 atacaatgag gataagcaaa acgcaaacta taaagatgaa aatgtatgca attgtcatga
     2581 taaaatattt tattgattat tcattaattt ctttatctca aaaatttggt tggcaaagta
     2641 agtacttttt tcgggaaatt tcaaacttaa ttttgaatat gtgtctatcg ctttttgata
     2701 attcttttga cggacaaata ttttggccag cgtctcagtc ataaaatcat catgttcttc
     2761 tacgctattt gtcgaaatat catattcaac aaattcttct acttgctttg gcctgatttt
     2821 tggcatatct gctaaaaatt tgtcaatcag gtcaggttca ggccctgtaa tttctgattg
     2881 gcctgttact ccatcttccg ggcccccaat gtccaaactt atattttctg ataaatcatt
     2941 tttattgtta gctttttcag gactaaatct caatgcggaa ttgtctgttt ttttttcaaa
     3001 aaactcaaac ccaacacctc taacatcttg cttaatctgt tcatcttcaa tgataagtat
     3061 agattggtct attctttctg tgtccaaaat atcattttca attgcaaaat caacagtagc
     3121 aacatcttta ggcctgatta tttcaatact ttcatcaagt tcaaaagtaa tttcaggcag
     3181 taaacgttgc tcaaatccag tttctgtttt tcctgaaatt tgttgcgaaa cggcatcggc
     3241 aatagcatca ctcaaaaact ccttctcttc ctttcgtatc gagggctcag ctggttctat
     3301 cccactattt tcaacaggtt tttctgtcaa tattgtctta gcatgagata aattgatata
     3361 tttaaagaga tgtttacgat ttgaaacatg aacaacagct tttttcaaca ggttagcata
     3421 ttcagcttca tctttctctt tgtaatacct gagtaacaaa aaatagattg gctgaaagta
     3481 tggatactgg tttagaatac aattcaaact ttcaacatca acggcttcta aagcttttac
     3541 attattcaat agtgataata tgtcctcttt gttgagctcc atagtttacc agtttgtaaa
     3601 tgctttattg aaaatatctt cggcaaggtt atcaagaatt atcgggaaca acctgttttc
     3661 tgcagactcc aacgactcgc cgctactaac atcgtagtcc tcgtacctcg aaaatgacga
     3721 tgaaaaattg tattgtggtt caacagtatt gacaaacttc acattgacag ttacttttaa
     3781 ccgctctttt gtaaccttgt cgctcgattg taaagcctgt gtttcaacag tgtagccagt
     3841 tatctcgcct tcaaagctaa catcgccgcc atctgtaaca aagtatagtt tagtattttg
     3901 ctctatcttt tcacgcaatt tattagtcag gtcgtaggcc atggtggctc gtactgttcc
     3961 gcccctgttt tgaaaagttg aaatagatac tgtctttaca tcgggagata ttgaagcccc
     4021 gctggtagag tattttaccg taaagcatga ttgcacaaca agcataattg ctgcaaaaac
     4081 aataagtaaa aatctatatt ttttttcaaa aggcctcatt ccatgttata ttctttaatc
     4141 tttctgtata atgtcctttc cgaaataccc agttcgtcgg cagcaagttt gcgtttccct
     4201 ccatgttttt caagggcttt tttgattagc tctttttctt tctcttgtaa tgaaagggtt
     4261 tcttcaatga cctcttgcgt ttcttcaatg accggactgg ttttcttaat atcataggtt
     4321 ttaacaaaag aattagtatc aaaatcaagg ttcttaaaaa cactactctt gtctttgctg
     4381 ttatcaaagg ggtgttcctc gttaaaaggc ctgttgtgcg tcaattcaaa tacagtcttt
     4441 ttcaattcgt gcaaatctgc cttcatatca aaaagtattt tgtacagaat ttcacgttct
     4501 gaagcaaacg agctgtcagg ctcatttctt ttaaacaaca tcgggagccg ctcactttga
     4561 taatctggca ggtacaatct taaagtggtt tcatcaatta cccttttttt ctcaatgata
     4621 gaaatctgct cggctatgtt tttcaattga cgtacattgc caggccaagt gtatgtttct
     4681 aaaaccttta cagcctcagg ttcaagactt atagcaggca tacgatactt ttcggcaaag
     4741 tcagccgcaa attttttaaa cagcaaatga atatcttctg ctcgctggtt taaaggcgga
     4801 acaaatatgg gaactgtatt taacctgtaa tacaagtctt ctcgaaactt gccattatca
     4861 atagctttag ggatatctac attcgtggcc gctacaacac gtacattggt cttttgaact
     4921 ttcgatgaac ctactttcaa aaattcgccg gtttcaagca ccctcaaaag tcggacctgt
     4981 gtggacaatg gcaactctgc cacttcatca agaaaaatgg ttcctccgtt ggctacttca
     5041 aaataccctt tgcggctttc gtgcgcccct gtaaaagagc ccttttcatg cccaaaaagc
     5101 tcagagtcaa tagtaccctc aggtattgca ccacagttta cagcaatata ctggccatgc
     5161 ttgcgtgcac taaacgaatg gatgatttgc ggaaaaacct ctttacctgc accactggga
     5221 cctgttataa gcaccgacaa atcagtaggc gcaacctgcc ttgcaatatc aatggccctg
     5281 ttaagggcat tgttattgcc tattatccca aaccgctgct ttattgatgt aacatccatg
     5341 ttttcaaaaa taactgacaa aattaacctt aatgctgaca aaattgcaat tttatcggca
     5401 tttttaattt aatcgtgaaa cttgattttg aaaaatgacc taatagcctc ttattccttc
     5461 ctttagcctt tgcctttctt ttattttact tcttcaaaat caacataatc accgtcatct
     5521 ttattaaacc gtttcttctt agaattattt tcaacagtag tattatcaaa ataatgataa
     5581 tcaccttttg attttttgtg attgtcgctc ctgaaaatgt gtataagacg caaaatagct
     5641 tttattagtg tataagcaag aaaaaatata aaaaggagaa gaaaaaattt tttcatccgt
     5701 tttgtaaaat tttttgacaa aattacgttt tttccacgct ttatatgtgt agatatttta
     5761 aaaaagaagt tttaataaac cttaaaaaat acatttagat caacaaaaag cattaatttt
     5821 atagcattcg tatttgcatt tactcaacat aaaaaaatat acatagcatg agcgaatttt
     5881 taaaaaaacg aggaacgata gctatcctga caggaggagg agatgtaccg ggcttaaacc
     5941 ctgctattag ggccattact ctcagggcaa tagaagaagg gtaccaggtg attggcttaa
     6001 aacgcggatg gcttggtata accgagttga ttcgtgacaa aaatgctgat aacagcaaca
     6061 attacatggt gctatcagaa gaaatagtaa ataaatcagg gcgtaccgga gggtcttttc
     6121 ttcatacttc acgtaccagg ccaagccatt ttgccaaaga tttagtcccg gttcatttgc
     6181 aaagcaaata caataaagaa atcaatgact taactcccga agtgttaaaa aacttagatt
     6241 accttggggt cgattatatt atccctattg ggggtgacga aacgcttagt tatgcagtac
     6301 gcatgtgtca ggaaggggtt aaagttatag ctattcctaa aaccatggat aacgacgtcc
     6361 cgggaaccga ctactgcata ggttttagca cctgcataac ccgtaccatt agtatggtca
     6421 ataccttgag gactactgca ggttcgcacg aacgaatact tgttgttgaa atatttggcc
     6481 gatatgccgg ctttacggct atgttgccta ccatggcagg agctgcttcg cgctgtgtaa
     6541 tccctgaaca taagttcaat atcgaaaaac ttaccgaaat aatggtccat gaccgcaata
     6601 ctaatcccag caagtactct gtactattag tttctgaggg tgctactatg gaagggggcg
     6661 atgaaaaggt aatgccaacc aaagaaagcg aaatgcacct taatgaaagt attggcagta
     6721 ttggagatgt agtgtctcga aaaattaggg aattgtcgcc caaatataac aatgggaaaa
     6781 ccatcaatgt cattgagcaa aaccttgggt accttgttcg tggtggcgac cctgatgcca
     6841 ttgatgccat tgtcccaatg gcttttggga atttggcgct cgaccttata cttaaaggta
     6901 ttcacggccg attggtagta ctgcgaaacg gacgctatga caatgcccct ctggatgtga
     6961 ttaccagcca gcgcaaaaca gttgatgtca aacgttttta caataccgaa cgctacaggc
     7021 cacattataa gagttttgaa tttcagcctt tgtttatcat gaccggaaac cttagctaaa
     7081 caaaagggta ttttgctgaa gatataattt agcctttctt tgcagaaagg cttttttgtt
     7141 ttattgaatc agacatcttc aggtttatca tggaggagta tgttatgcag ccaaagatta
     7201 gttgggtttt tgccctgtag gggttgaaaa agagcagcgt agttgcgaaa tatttgtaga
     7261 aacaaaaata tagaatggag gattaaaggt gcgtagcacc aaaatatatg tcgccactac
     7321 gtggctttca ttcaataaat taacgttagt tcaatgaaag aataatattg tcaactattg
     7381 tttaaaaagc attgaattgc aataaatact aattttctat gcaacttttt
//
LOCUS       DAOSPF010000116         7178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_10443_length_7178_cov_8.929524, whole genome shotgun sequence.
ACCESSION   DAOSPF010000116 DAOSPF010000000
VERSION     DAOSPF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10443_length_7178_cov_8.929524"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(692..1234)
                     /locus_tag="PK252_14150"
     CDS             complement(692..1234)
                     /locus_tag="PK252_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT15895.1"
                     /translation="MPEITFAHPANIFKFCPKCGKEGFAFDGEKAFDCHHCHFRFYIN
                     ASTAVAVILELPSGEIILTKRKNEPKAGYYDLPGGFVDPGESAEHAAIRELQEELGIQ
                     VNNLKFIASFPNEYIFGGITYYTTDISFVASIPQGTQLSPDDDVAEVLVIKPENIDFN
                     IISFPSVVNVLKEYRKSLKI"
     gene            complement(1239..1550)
                     /locus_tag="PK252_14155"
     CDS             complement(1239..1550)
                     /locus_tag="PK252_14155"
                     /inference="COORDINATES: protein motif:HMM:NF025903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOT15896.1"
                     /translation="MDAVVEILQLPADDKNIRVLEYEPEYFQMKPPYEMLIEISMFAG
                     RTKETKKKLFQTIVNNLSAKGLISKEKVFILLNEQPMENWGVKGGIPADDINLGFKIN
                     V"
     gene            complement(1886..3352)
                     /gene="cysS"
                     /locus_tag="PK252_14160"
     CDS             complement(1886..3352)
                     /gene="cysS"
                     /locus_tag="PK252_14160"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT15897.1"
                     /translation="MDMELYLYNTLSRKKELFEPINPPFAGMYVCGPTVYGDAHLGHA
                     RPAITFDLLFRYLQHLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLERLEPMEVV
                     QYFTNRYHDCMKKLNVLPPSIEPHASGHIIEQQEMVQQILDNGYAYESNGSIYFDVEK
                     YNKQHTYGILSGRVLDDLMANTRQLEGQDEKRNPFDFALWKKASPYHIMRWPSKWSDG
                     FPGWHLECSVMSSKYLGEKFDIHGGGMDLVFPHHECEIAQTVAAHGHHSVKYWLHNNM
                     ITINGQKMGKSLGNFITLDEFFEGSHKLLEQAYSPMTIRFFILQAHYRSTVDFSNEAL
                     VAASKGLQRLMEANKALQTLKASGNNSNDIDSIEKNCYAAMNDDLNSALLIAHLMEAV
                     RIINAVYDGKEGISELQLNKLKNIFKIFVFDILGLTDESSNNHSEIIGSLVDMVQQMR
                     MKAKAEKDYQTSDNLRNKLGEIGIEIKDRKDGYDWKLK"
     gene            3456..3746
                     /locus_tag="PK252_14165"
     CDS             3456..3746
                     /locus_tag="PK252_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT15898.1"
                     /translation="MIIRSEIEQKLKALKPSLAKHFYVDKIGYFGSFARNEQTENSDI
                     DIIVSFKKPLGWAFFDLQDLLEKELQLKVDLVSDKALKDQLRDAILKKVRYV"
     gene            complement(4108..4281)
                     /locus_tag="PK252_14170"
     CDS             complement(4108..4281)
                     /locus_tag="PK252_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT15899.1"
                     /translation="MSAEKVLETMKKVGKPMKSAEIATATGIDKAEVDKIIKKLKQED
                     KVFSPKVCFYQAK"
     gene            4346..5038
                     /gene="mtgA"
                     /locus_tag="PK252_14175"
     CDS             4346..5038
                     /gene="mtgA"
                     /locus_tag="PK252_14175"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOT15900.1"
                     /translation="MKSFINRLFKLFAITMLIALLFSISWVILYKFVDPPLTVLMVKR
                     MFQHDEKKGDFRFEKDWADIDEISPNMVQAVVAAEDNNFMTHFGVDWEAIQKARQINK
                     HSRIKHGASTITQQTAKNVFLFPSRTYFRKGLELYFTFLIEICWSKERIMEVYLNVIE
                     MGRGIYGVEAASQFYYKKSAKKLSKREAAMFATILPSPLKRNPAKPSRYMVAFQARII
                     GLMDKIGPVELK"
     gene            5386..6483
                     /gene="meaB"
                     /locus_tag="PK252_14180"
     CDS             5386..6483
                     /gene="meaB"
                     /locus_tag="PK252_14180"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT15901.1"
                     /translation="MSDPLLPNKTALTVNDGIPQPPSVNEEMVQRLKQHKRREPSVEE
                     IIEGIKKGDRGLLSRGITLVESNLPRHQEMARTIVDQCIGYSGKSIRIGITGVPGVGK
                     SSFIEAFGTYITSLGHKMAVLAIDPSSERSKGSILGDKTRMVELSANANAFIRPSPSA
                     GALGGVARKTRETILLCEAAGFDVILVETVGVGQSETAVHSMVDYFLLLALAGAGDEL
                     QGIKRGIMEMADTIVITKADGTNIDKAQRAKSEIAAALHFMPCPPSGWRPDVLTCSAH
                     NNIGIDKIWETVLKFKSFVTQSGYFAQNRNEQARYWMHQTIRDELNHRFYHDKELHHE
                     ISVMEKDVVNGKISSFEAARLLLDKYFWEKK"
     gene            complement(6537..7082)
                     /locus_tag="PK252_14185"
     CDS             complement(6537..7082)
                     /locus_tag="PK252_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT15902.1"
                     /translation="MFVGGAFSVEMDKTKNATEDIYKRTSFGITPKVGYYLNDQFAIG
                     GAIGYTTSSYTYFYGAGDADDTKSTSNNMSISPFIRYHAVEAGNFSLFVEPMLSIGFG
                     SSKSERGSTTTSDADHSSFGIQISPAMSYKINDKINIEAYFGGLSYSSSTEKEGDNKR
                     TATSFGLEFSSRLALGFIYKF"
     gene            complement(6995..>7178)
                     /locus_tag="PK252_14190"
     CDS             complement(6995..>7178)
                     /locus_tag="PK252_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15903.1"
                     /translation="YSPLEQPTAFRRVVHEFYYLLLSLLAFRLFLKCLLEVLFLLKWI
                     KLKMLQKTSIKGLALA"
BASE COUNT         2148 a         1389 c         1387 g         2254 t
ORIGIN      
        1 gactttttgc ggctttcaga aagggttggt gctaaatacc gacaaagtcg ggaaatatat
       61 caataatcaa gaatcgcatc acaagaagat atctttcaaa gaagaatatc ttgtgtttct
      121 taataaatat caagtggaat acagtgaaca atttttgttt gaattttatg aatagggctc
      181 atcttcaatc tttaaaatct ttctgcattc ttttaacaca ttgacaactg atggcttggg
      241 tcgctcctat ggagcttaat taagagccgg agaaaacttt ctataaacag caagctccta
      301 cggagctaaa ggccgtttag ctaatctttc agcacaaaat taatatttca tattttgccc
      361 catcggggca tcctgtttat aaaaattgca acaattacta taggttagct ccgtaggagc
      421 gtcctgaaaa accaacctta aatctttaaa ctctttctgt actctttcaa cacattgaca
      481 actgatggct tgggtcgctc ctatggagct taattaagag ccggagaaaa ctttctataa
      541 acagcaagct cctacggagc taaaggccgt ttagctaatc tttcagcaca aaattaatat
      601 ttcatatttt gccccatcgg ggcatcctgt ttataaaaat tgcaacaatt actataggtt
      661 agctccgtag gagcgtcctg aaaaaccaac cttaaatctt taaactcttt ctgtactctt
      721 tcaacacatt gacaactgaa ggaaaactga tgatattgaa atcaatgttt tcaggcttta
      781 taactaaaac ttcggcaaca tcatcgtcag ggctaagctg agtgccttgc ggaatacttg
      841 ccacaaaaga aatatctgtg gtataatatg ttatacctcc aaaaatatac tcattaggga
      901 aagaggcaat aaatttaagg ttattaactt gtatgccaag ctcttcctga agttctctga
      961 tggcagcatg ctcggcactt tctcccggat caacaaatcc tccggggagg tcgtagtatc
     1021 ctgccttggg ttcattctta cgttttgtaa gtattatttc tcccgaaggt aactctaata
     1081 tcactgcaac tgcggtggat gcattaatgt agaacctgaa atggcaatga tggcaatcaa
     1141 aagctttttc tccatcaaaa gcgaaccctt ctttgccaca tttggggcaa aatttaaaga
     1201 tattggccgg gtgagcgaat gttatttctg gcatttgttt atacatttat tttaaaccca
     1261 aggtttatat catcagcagg aattccacct tttacacccc agttctccat gggctgttca
     1321 tttagcagga tgaatacttt ctcttttgat ataagccctt tagcagataa attattcaca
     1381 atggtttgaa aaagcttctt tttcgtttcc ttagtcctgc cagcgaacat agatatttca
     1441 atcaacattt cataaggtgg tttcatttga aaatactcag gttcgtactc gagtacgcgg
     1501 atattcttat catcagcggg caattgcaat atttcaacta cagcatccat caccaaatca
     1561 ggatttgtgc aatgacttgt ttgtcatttc tttttgttag ttcaactttt actaggggca
     1621 taatcaatta cgaattacaa atgactaatt actgtgtttt aatcccctct tttacaagga
     1681 ggggtaaggg gtatagtcgt caacctaaga attatttttg gcttttaacc tcccagccct
     1741 ccttaaaaag gagggctaac aaagttgtgg taccccaaaa tagcactgct ctgttaaagt
     1801 acctttttaa ggggtttggg gttagataaa gccgaggttg acggctatgg aggtaaggtg
     1861 tggtatatat ttttaccact tcctcctact tcaacttcca gtcgtaaccg tccttgcggt
     1921 ctttgatttc tatacctatc tcgccaagtt tgttgcgcaa gttgtctgag gtttgatagt
     1981 ccttttcagc tttagctttc atgcgcattt gctgtaccat atctaccaaa gaacctatga
     2041 tttctgaatg gttgttgctg ctttcgtctg ttaagccaag aatatcgaat acaaaaattt
     2101 tgaaaatatt cttcaattta tttaactgga gttcgctaat gccttctttc ccatcataga
     2161 cagcattgat gatacgaaca gcttccatca ggtgagctat aagcaaagca ctattcaggt
     2221 cgtcattcat ggcagcatag cagtttttct caatgctgtc aatatcattg ctgttgttgc
     2281 ccgaagcttt taaggtttgt agtgccttat ttgcttccat caatctttgt aaaccttttg
     2341 aggcagcaac caaagcttcg ttagagaaat caactgtact acgatagtgc gcctgtaaaa
     2401 taaagaacct gattgtcatt ggagaataag cctgctcgag gagcttatgg cttccttcaa
     2461 agaattcatc cagtgtgata aagttaccca aggatttgcc cattttctgg ccattgatgg
     2521 taatcatatt attgtgcagc cagtatttaa cagaatgatg tccgtgagcg gcaacagtct
     2581 gcgctatttc acattcgtga tgcgggaaaa ccaggtccat gccgccaccg tgaatgtcaa
     2641 acttttcgcc aagatatttg ctgctcatca cagaacattc gaggtgccaa cccgggaaac
     2701 catcgctcca tttcgaaggc catcgcatga tatgataagg gcttgctttt ttccaaagtg
     2761 caaaatcaaa cggattgcgc ttttcatctt gcccttcgag ctggcgggtg ttggccataa
     2821 ggtcgtcaag cacacgtccc gaaaggattc cataagtatg ttgtttgttg tacttttcta
     2881 catcaaagta aatggagcca ttgctttcgt aagcatagcc attatctaag atttgctgaa
     2941 ccatctcctg ctgctcaatg atatggcctg atgcatgagg ttcaatgctg ggaggcaata
     3001 cattcagctt tttcatgcaa tcgtgataac gattggtaaa atattggact acttccatag
     3061 gttcaagccg ctcaagacgt gcttttttgg ctattttatc ttcaccttcg tctgcatcat
     3121 tctccaaatg gccaacatcg gtgatgttgc gtacgtaacg cactttgtat cccaagtgtt
     3181 gaagataacg aaacagtaag tcaaaggtaa tggccggacg ggcatgtcct aaatgtgcat
     3241 cgccatatac tgttgggcca caaacgtaca tgcctgcaaa tggtggatta ataggctcaa
     3301 ataattcttt tttacgactg agcgtattgt ataagtataa ttccatatca attgaaaaat
     3361 atggacaaaa gtagcaattt tttatttgac agttttctca ttatcaatag aacctcaact
     3421 tgtttaacat aaatgaatgc tttatatttg tatctatgat aattaggtct gaaatagagc
     3481 aaaaattaaa ggcactgaag ccctctttag ctaaacattt ttatgtggat aaaattggct
     3541 attttggttc ttttgcccgt aatgaacaaa ctgaaaattc tgatatcgat attattgttt
     3601 cttttaaaaa gcctttagga tgggcatttt ttgatttaca agatttgctt gaaaaagaat
     3661 tgcagctaaa agtagatttg gtttcagata aagctttaaa agatcagctt agagatgcta
     3721 ttctgaaaaa agttcgctat gtatgaagcc tgaagacaga aaatatcatt tttatcttga
     3781 ggatattatt gtttcgatga aacgtattca ggaatacatt gaaggcattg attttccata
     3841 atttaaacgt aaatatatca ttgtagatgc ggtaatccgt aattttgaaa ttataagtga
     3901 agcaacaaaa cacataccca aagaaatact ggataaatat cctaatattc cctggcgaaa
     3961 aatgtatagt cttcgaaata ttgtgagcta cgaatatttc ggtatagatt atgagatgat
     4021 ttgggagata gccaaaaatg agattccttc aaatttagct gatttagaag tcattataaa
     4081 aatagaaaaa gacaaataat tccttagcta tttagcctga taaaaacaga cttttggcga
     4141 aaacaccttg tcttcctgct ttagtttttt aatgattttg tccacttcag ctttgtcaat
     4201 gcccgtagct gtggctattt ctgccgactt catcggtttg ccgacttttt tcatggtttc
     4261 taaaactttt tctgcgctca tactattttt ttttaagtga atgttgtaat gtaaaaatat
     4321 atctttgtgc taatttttta gcataatgaa atcctttatt aacagactgt ttaagctttt
     4381 tgctataaca atgctgatag cacttttgtt cagtatttca tgggtaatat tatacaaatt
     4441 tgtggacccg cctctgacag tactgatggt taagcgaatg ttccaacatg atgaaaaaaa
     4501 aggagacttc aggtttgaaa aagattgggc ggacattgac gaaatttcgc ctaacatggt
     4561 acaagcagtt gttgctgctg aggacaacaa tttcatgacc cactttgggg tagattggga
     4621 agccattcaa aaagccaggc aaataaacaa acacagccgt ataaaacacg gagcgagtac
     4681 tattacccaa caaaccgcca agaatgtttt tcttttccct tcacgtacct attttcgtaa
     4741 agggttggag ctttatttta cctttctcat tgagatttgc tggagtaaag aacgcattat
     4801 ggaggtttac cttaatgtta tagagatggg acgcggaata tacggggttg aagccgcttc
     4861 tcaattttat tataaaaaat cggccaagaa attatcgaaa cgagaagcag ctatgtttgc
     4921 tactattttg ccatcgcctc tcaagcgaaa tcctgccaaa ccatcgcgat acatggtagc
     4981 ctttcaggcc cgcatcattg ggctcatgga taaaattggc ccggtggaac taaaataata
     5041 tctttgttat tcaatacaga gaatatttgt tgttattcgc gatttataca acatattaca
     5101 ttaacgttaa aagaaacttt accaatcaag aggtccactt agaacattga gaaattcgct
     5161 tttagcgaat tttatactct gtgtccttta tgtactcttt ttaaatatgt cattcaaagt
     5221 ttgtaatcag ttaaatgaac aaactatatt atttgtttga aatttgaact ctgtgtttaa
     5281 caaattgtct ttttgttatc gtctgtcaat attttgttta ttctttgaac cagcatttga
     5341 ctacttcggc atttttgaat gcttactttt acaaaaaaat gtaacatgtc tgacccatta
     5401 ctgccaaata aaactgcctt aaccgtcaat gatggaattc ctcagcctcc gtctgtcaat
     5461 gaggagatgg tacaaaggct gaaacagcat aaacgccgtg agccttcagt ggaagaaatt
     5521 attgagggta ttaaaaaagg cgaccggggc ttgcttagcc gtggaataac ccttgtcgaa
     5581 agcaacctgc cacgtcatca ggaaatggca cgcactattg ttgaccagtg cattggttat
     5641 tcgggcaaat ctatcagaat tgggattact ggcgtacctg gcgttgggaa aagctcattt
     5701 attgaagcat ttggaactta catcacgtct cttggccata aaatggcagt tttggctatc
     5761 gaccccagca gtgagcgctc aaaagggagt attttggggg ataagacccg aatggttgaa
     5821 ctttcggcca atgccaatgc attcattcgc ccttcaccat ctgccggggc tcttggcggt
     5881 gttgcccgaa aaacccgcga aactatttta ctttgcgaag ctgccggatt tgacgtaata
     5941 ttggtcgaaa cggtaggcgt aggccagagc gaaacagccg tgcattctat ggttgattat
     6001 ttccttcttt tggcattagc tggagcaggc gatgaattgc agggtatcaa acgcggcatc
     6061 atggaaatgg ccgacaccat tgttattacc aaagcagatg gaacaaatat agataaagcg
     6121 caaagggcaa aatcggagat tgccgctgca ttacatttca tgccttgtcc tccatcaggc
     6181 tggagacctg atgtattaac ctgttcggca cataacaaca ttggtattga caaaatctgg
     6241 gaaacagttc tcaaatttaa aagtttcgtt acccaatccg gctattttgc ccaaaaccgt
     6301 aacgaacaag cgcgctattg gatgcaccag actattcgcg atgagctcaa ccacaggttt
     6361 tatcacgata aagagctgca ccacgagatt tcagtcatgg aaaaagacgt ggtgaacgga
     6421 aaaatcagta gtttcgaggc tgccaggtta ttgttggata agtatttttg ggaaaagaaa
     6481 taggttgaaa acaaaaaagg ccaccatcgg cagccttttt aatgtttttt ctacaattag
     6541 aatttgtaaa taaagccaag tgctaaacgg cttgaaaatt ccaatccaaa actagtagca
     6601 gttcttttgt tatctccttc tttttctgta gagcttgaat agcttagccc gccaaaataa
     6661 gcttcgatgt taattttgtc attgattttg tatgacatgg caggtgatat ttgtattcca
     6721 aaacttgaat gatcagcgtc actggtagta gtgcttcctc tttcgctttt actgctgcca
     6781 aagccgatgc ttaacattgg ttcaacaaac aacgaaaaat tgccagcttc tacagcatga
     6841 tagcggataa aaggagaaat agacatatta ttgcttgttg atttagtatc atcagcatca
     6901 ccagcaccat agaaataagt atatgagctt gtagtgtaac caatagcacc tccaatagcg
     6961 aactggtcat tcaaataata accaactttt ggggttatgc caaagctagt ccttttatag
     7021 atgtcttctg tagcattttt agttttatcc atttcaacag aaaaagcacc tccaacaaac
     7081 atttgagaaa aagcctgaac gctaacagcg acaacagcag ataataaaac tcatgtacca
     7141 cacgcctgaa ggcggtgggt tgttctaacg gactgtaa
//
LOCUS       DAOSPF010000117         6790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_11274_length_6790_cov_8.128582, whole genome shotgun sequence.
ACCESSION   DAOSPF010000117 DAOSPF010000000
VERSION     DAOSPF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11274_length_6790_cov_8.128582"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK252_14195"
     CDS             complement(<1..390)
                     /locus_tag="PK252_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOT15904.1"
                     /translation="MSLYYRIKLVFCANRLHREVASLLGYFPKNWDIYEMAFTHRSAS
                     VVLANGSSVNNERLEFLGDAILDAIIADYLFSYYPNKDEGFLSKMRSKIVKRQQLNVM
                     ASALGLDSLIIKGCINNNGGKYIVGNTL"
     gene            complement(394..1641)
                     /gene="fabF"
                     /locus_tag="PK252_14200"
     CDS             complement(394..1641)
                     /gene="fabF"
                     /locus_tag="PK252_14200"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT15905.1"
                     /translation="MELKRVVVTGLGAITPVGNTVEEFWNALINGESGAAPITRFNPE
                     KFKTQFACEVKNYNPEKYFDRKEIRKIDLYAQFAMIAADEAMKDSGLDLSMTNLDRIG
                     VIIASGIGGIDTFYQECRNYILGDGSPKISPFFVPKMICDIAAGHVSMKYGLRGPNYA
                     TVSACASSTHALVDAFNLIRLGKANGFLVGGAEAAINEISMGGFNSLQALSTRNEDPK
                     TASRPFDADRDGFVMGEGAGILFFEELEHAKARGARIYAEVIGGGMSADAHHITAPHP
                     EGAGAILTMTNAIEDAGIKPSEVDYINVHGTATPLGDVAELKAIKAIFGEHAYKMNIS
                     STKSMTGHLLGATGAIETIASILAVKNDIVPPTINVQNLDPEIDPKLNLTLNKAQKRT
                     VNVALSNTFGFGGHNSSIIVRKF"
     gene            complement(1666..1902)
                     /locus_tag="PK252_14205"
     CDS             complement(1666..1902)
                     /locus_tag="PK252_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT15906.1"
                     /translation="MSDIASRVKSIIVDKLGVDENEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAENISTVGDAIKFIEANVK"
     gene            2102..2701
                     /gene="purN"
                     /locus_tag="PK252_14210"
     CDS             2102..2701
                     /gene="purN"
                     /locus_tag="PK252_14210"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT15907.1"
                     /translation="MNNIAIFASGSGSNAQAIVEYFRSLGENPVKIILSNNKNAFVIE
                     RAKTLGVECIIFNKDDFYHSSRVIDILKERNIDFIVLAGFLWLIPNSLIDAYANKIIN
                     IHPALLPKYGGKGMYGMHVHEAVVQNKEAYSGITIHFVDEKYDNGAIIFQAQCKIEEG
                     DTAEMVAQKVHQLEYAYFPKVVRQLVLNRPIEEVLGVNQ"
     gene            complement(2691..3554)
                     /locus_tag="PK252_14215"
     CDS             complement(2691..3554)
                     /locus_tag="PK252_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOT15908.1"
                     /translation="MEKVKIGIIQTSCTANKEANINKAIGQIKKLASEGAQIICLQEL
                     FSTLYFCDIENHDNFALAETIPGPSTDIFSKVAAENKVVLIVSLFEKRTAGLYHNTAA
                     VIDADGSILGIYRKSHIPDDPGFYEKFYFAPGDTGYKAFDTKYGRIGVLICWDQWYPE
                     AARITSLMGAKVLFYPTAIGWELDTSAEINNEQYQAWQTIQRSHAIANGVHVVSVNRV
                     GTESGTKFWGGSFVANPFGSLLYLGPHDKEESKVVEIDLDATENYRRVWPFLRDRRID
                     NYAPIINRFID"
     gene            3825..5780
                     /locus_tag="PK252_14220"
     CDS             3825..5780
                     /locus_tag="PK252_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT15909.1"
                     /translation="MVSVSQVGLSFGAFELFSDVTFMVNPKDRIGLTGKNGAGKSTLL
                     KMIAGLQKPDTGVITIPEGFRIGYLPQYMVTCNNNTVLAETSLAFSEILLLEKEIDTL
                     NIELSSRTDYESDDYHNLIVKLNELTERHNILGAGSLQSSIEKTLLGLGFSIADFHRP
                     TSSLSGGWRMRIELAKILLNNSDLLLLDEPTNHLDIESIQWLEDYLVSFPGALILISH
                     DRKFLDNVTNRTIELSLGKAYDYKVSYSRYVELRRERREQQMAAFRNQQKMIQDTEDF
                     IERFRYKATKAVQVQSRIKQLEKLDRIEVEEEDLSTINIRFPTPVKSGKIALDIKKIS
                     KSYGSKLVLKEVDLILESGRRVAFVGKNGEGKSTLSKIIVNEIHDYEGTIKLGHNIKI
                     GYFAQNQDELMDGDRSVFDIIDQVAVGDIRTKIRDILGAFLFRGDDVFKKVKVLSGGE
                     RSRLAIAKLLLEPYNLLVLDEPTNHLDMRSKDVLKQALLNYEGTLIIVSHDRDFLDGL
                     VDTVYEFGGQRIKEHIGGIYEFLERKKLQTLQSLNTSVLQIPVQQNNKDVQQKGKLQY
                     AEKKEFDKIVRKINGQISDSENKIQKLEMQIEIANKKLADPAFLESNGNDSNFFKEYD
                     DLKLKLQQEMENWEQLHEELEKMMTND"
     gene            5942..6703
                     /gene="rlmB"
                     /locus_tag="PK252_14225"
     CDS             5942..6703
                     /gene="rlmB"
                     /locus_tag="PK252_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT15910.1"
                     /translation="MFNRDKQPQPKLVFGIHAVLEAVRSGKEIEKLIIKKEMSGEALR
                     ELMKACRERNIPYQFVPVDRFDRITRKNHQGLIAYMAEIEYQPLEMVVSAVFEDGREP
                     AILILDHITDVRNFGSIARTAECAGFDAIAIPDKGAAQINSDAIKTSSGALHNINICR
                     VTNLTNAVKYLKDCGLKVFAATEKAKQPYYKTNMKGPVAIVLGAEDAGISPEILRYAD
                     ELVQIPMIGKTSSLNVSVAAGIIIYEVVKQRLSDF"
BASE COUNT         2098 a         1188 c         1339 g         2165 t
ORIGIN      
        1 caatgtatta ccaacaatat actttccgcc gttattgttg atacatcctt ttattataag
       61 gctatctaaa cctaatgctg aagccataac attcaattgt tgccttttga caatttttga
      121 acgcattttt gataaaaaac cttcgtcttt atttggataa tacgaaaaaa gataatcagc
      181 aattattgca tctaatattg catctccaag aaattcaagc ctttcattat tgactgaaga
      241 gccattggct aaaaccacag atgccgaacg atgagtaaaa gccatttcgt atatatccca
      301 atttttggga aaatatccta acaagcttgc aacttctcga tgaagcctgt tagcacaaaa
      361 tacaagcttt attcggtagt atagagacaa aaactaaaat tttcttacaa taatagaaga
      421 gttgtggcca ccaaatccaa atgtattact taaagctaca ttgactgttc gtttttgagc
      481 tttgttaaga gtaaggttga gctttggatc aatttccgga tctaaatttt gaacattgat
      541 agtaggtgga acaatatcat ttttaacggc caaaattgat gcaatggttt caatagcccc
      601 tgttgcgcca agtaagtggc ctgtcattga ttttgttgag ctaatgttca tcttataagc
      661 atgttcgcca aaaatagctt tgatagcttt taattctgct acatctccaa gtggagtagc
      721 tgtaccatga acgttgatgt aatcaacctc tgaaggtttg attccggcat cttcgatagc
      781 gttagtcatg gtaagtatag ctcctgcccc ttcagggtga ggagcagtaa tatgatgggc
      841 atcagcagac atgccacctc cgattacttc ggcataaata cgggcaccgc gagcttttgc
      901 atgctcaagt tcttcaaaga aaagaatccc ggcaccttct cccattacaa aaccatcacg
      961 atcggcatca aaagggcgcg atgccgtttt cggatcttcg tttcgggtcg agagtgcttg
     1021 taatgagttg aaccctccca tagagatttc attaatggca gcttctgctc ctcctacaag
     1081 gaacccgttg gctttaccta acctgataag gttaaaagca tctaccaatg catgagtaga
     1141 ggaagcacat gcagacactg ttgcataatt aggccctctt aacccatatt tcatagaaac
     1201 atggcctgct gcaatgtcgc aaatcatctt tggtacaaag aatgggctaa tttttggact
     1261 gccatcgcct aaaatgtaat tacgacattc ctgatagaag gtgtcaatac ctccaatacc
     1321 ggaagctatg ataacaccaa ttctatcaag gtttgtcata gaaaggtcca atccactatc
     1381 tttcatcgcc tcatcggcag ctatcatagc aaattgtgcg tataagtcta tttttcttat
     1441 ttcctttctg tcaaaatatt tttcagggtt gtaatttttt acttcacaag caaattgagt
     1501 tttgaatttt tccggattaa aacgagtgat aggggcagcg ccactttcac cattgattaa
     1561 tgcattccaa aattcttcaa ctgtatttcc tactggagta atggctccta aacctgtaac
     1621 aacaaccctt tttaattcca tttgagtaaa aatattttgc tttaattact taacatttgc
     1681 ttcgataaat ttgatagcgt cacctacagt actgatgttt tctgcttgat cgtcgggaat
     1741 tgcaatgttg aattcttttt cgaactccat aattaactct acagtgtcca aagagtctgc
     1801 tccgaggtcg ttggtaaagc tagcttctgg tgtaacttca ttttcatcaa cacctaattt
     1861 gtcaacgata atagatttta ctcttgatgc aatgtctgac ataattttct gaattagatt
     1921 aataaataat ttaactaaaa taaaaccgtg caaaggaaat catttctgga aaaattttca
     1981 aattattttt ataaaatttc aagtcaggat gtttttttat gtttttaaat ttttcttttg
     2041 gcttttcttt ttaagtttgc aagtttcttt ttttgacaaa aaacaaaata acttattgaa
     2101 aatgaataat atagccatat ttgcttctgg ttcgggctct aatgcacagg ctattgttga
     2161 gtattttagg agtcttggcg aaaatcctgt aaaaatcatt ctttctaata acaaaaatgc
     2221 ttttgttatt gaaagagcta aaactttggg cgttgaatgt attattttca ataaggacga
     2281 tttttatcat tcttccagag taattgatat tcttaaggaa cgtaacattg actttatagt
     2341 tttggctgga tttctgtggc ttatccctaa ctcgttaatt gacgcttatg ctaataaaat
     2401 aatcaatatt catccggctt tgttgcctaa atatggcgga aaaggcatgt atggtatgca
     2461 tgttcatgaa gctgttgtgc aaaataaaga ggcttactca ggtattacca ttcattttgt
     2521 tgacgaaaag tatgacaatg gtgctattat ttttcaggct caatgcaaaa ttgaagaggg
     2581 tgataccgca gaaatggtgg ctcaaaaagt tcatcagctg gaatatgcct atttccctaa
     2641 agtagtgcgt cagcttgtgc ttaataggcc tattgaagag gtgttaggag ttaatcaata
     2701 aacctgttta tgattggggc gtaattgtca atgcgcctgt cacgcaggaa aggccatact
     2761 cttcggtaat tttctgttgc gtcaaggtct atttctacaa cttttgattc ttctttgtca
     2821 tgtgggccaa ggtatagcaa tgacccaaaa ggattggcaa cgaaagaacc accccagaat
     2881 tttgtgcctg attcagtgcc aactctattt acagaaacta cgtgtacccc gttggcaatg
     2941 gcatgtgagc gttgtattgt ttgccatgct tgatattgct cgttatttat ttcggccgat
     3001 gtatctaatt cccatcctat ggctgttggg taaaataaca cctttgcacc cataagagag
     3061 gtaatccttg cagcttcagg ataccattga tcccagcata tcaggacgcc tatcctgcca
     3121 tatttggtgt caaaagcttt gtatccggta tctccgggag caaaataaaa tttttcgtaa
     3181 aaacccgggt catccggaat atgtgacttt cggtaaatgc ctaatatact tccatcagca
     3241 tcaattacag ctgcagtatt gtgatatagt ccggctgtac gtttctcgaa aagcgaaaca
     3301 ataagcacta ctttattttc agcagctact ttagaaaata tatctgtaga aggaccggga
     3361 attgtttctg ctaaagcaaa gttgtcgtga ttttcaatat cgcaaaagta aagcgtggaa
     3421 aaaagttcct gcaaacaaat aatttgagcg ccttcagaag caagcttttt tatttgccca
     3481 atcgctttgt tgatattggc ttctttgttg gctgtgcagc ttgtctgtat aattccaatt
     3541 tttacttttt ccatgtcgag aattttaggt tgtaaagata gtaaaaaaaa ggggcgttga
     3601 ttagggagta ggagataggg tatagaattt ttaatgaaat gagtagtgaa atgagatcaa
     3661 atttgatggt attatttgta ttctttaaaa ttaatattta taactaatta caatccccta
     3721 acccctaacc cctaacccct aacccttacc ctctactctt tataactccc aattcaatta
     3781 ccaacaattt tgactacttt tgccttttca aaataaataa gtaaatggtt tcggttagtc
     3841 aggtgggcct tagttttgga gcttttgagc tgtttagtga tgttacattt atggtaaacc
     3901 ctaaagacag gattgggctt accgggaaaa atggagctgg gaaatctaca ctacttaaga
     3961 tgattgccgg gcttcaaaag ccagatacag gtgtaattac tattcctgaa gggtttcgta
     4021 ttggttattt gccgcaatac atggttacct gcaataataa tacggtactt gccgaaacaa
     4081 gccttgcttt ttctgaaatt ttgttgcttg aaaaagaaat agatacttta aatattgagc
     4141 tttcttcacg taccgattat gaatctgatg attaccataa cctgatagtt aagctaaacg
     4201 aactgactga gagacataat attcttggcg caggtagttt acaaagctct atagagaaga
     4261 cgctccttgg ccttggtttt tcaatagctg attttcacag acctacatct tcattgtcgg
     4321 gcggttggcg tatgcgtatt gaacttgcaa aaatattgct taacaatagc gatttgttac
     4381 tgcttgatga gccaactaac catttggata ttgagtctat tcaatggttg gaagattatt
     4441 tggtttcgtt tcccggggcg ttgatattaa tttcgcacga ccgtaagttt ttagataatg
     4501 ttaccaatcg tactatcgag ctttctttag gtaaagctta tgattacaag gtttcatatt
     4561 caagatatgt tgaattacga agagaaagaa gggaacagca gatggcagct tttcgtaatc
     4621 aacaaaaaat gatacaagat acagaagatt ttattgaaag gtttagatat aaagctacca
     4681 aagctgttca ggttcaatcg cgtattaaac aactcgaaaa gcttgaccgc attgaggtag
     4741 aagaggaaga tctttctact atcaatataa ggttcccaac ccctgtaaaa tcagggaaaa
     4801 tagcgttgga tataaaaaaa attagtaaaa gctatggctc aaaacttgta ttaaaagaag
     4861 tagatcttat tttggaaagt ggcaggcgtg tggcttttgt tggcaagaat ggtgaaggga
     4921 agtcaacact ttcaaaaatc atagtcaacg aaatccatga ttatgaaggt acaatcaagc
     4981 ttggccataa tattaaaata ggctattttg cccaaaatca ggacgagctg atggatggcg
     5041 accgttctgt ttttgatatt attgaccagg tggctgttgg cgatataaga acaaagatac
     5101 gcgatattct tggcgctttt ctgttcaggg gtgatgatgt tttcaagaaa gtaaaggtgc
     5161 ttagtggtgg tgaacgttcg cgtttggcta tagctaagct tttgcttgaa ccatacaacc
     5221 ttcttgtgct tgatgagcct accaatcacc tcgatatgcg ttcgaaagac gtgttgaaac
     5281 aggcattgtt gaattatgaa ggtacactta tcattgtttc gcacgaccgt gactttttag
     5341 atgggctggt agatacagtt tatgaatttg gcgggcaacg aataaaagag catattggag
     5401 gtatttatga gttcttggaa agaaaaaagc ttcaaacgct tcaatctttg aatacctcag
     5461 tgttgcaaat tcctgtacaa caaaataata aagatgttca gcaaaaagga aagttgcaat
     5521 atgccgaaaa gaaggagttt gataaaattg tcagaaagat aaacggacag ataagtgata
     5581 gcgaaaataa aatccagaaa ttggagatgc agattgagat agcaaacaag aaattagcag
     5641 atccagcctt tttggagtca aatgggaatg attccaattt ctttaaggaa tatgacgact
     5701 taaagctaaa gttgcagcag gaaatggaga actgggagca gttgcatgaa gaactggaga
     5761 aaatgatgac taatgactaa tgaaacgagc atgatccgtt aaacacaaac aaggttgaaa
     5821 attcatcatg cgagttccat ccggcctcat ttaaaaaaaa tatttacgga tgaagacaag
     5881 gttatttatt catcagtcat ttgtaattag taattttaat gataattcat aattgatata
     5941 tatgtttaac agggataaac aacctcaacc caagctggta tttgggatac atgctgttct
     6001 tgaagctgtg cgttcaggga aagagataga gaagctcatt atcaagaagg aaatgtctgg
     6061 agaagcgctt cgcgagctta tgaaagcttg tagggaaagg aatatccctt accagtttgt
     6121 tcctgttgac cgttttgacc gtattacacg caagaatcat cagggattga tagcttatat
     6181 ggctgaaatt gagtatcaac cattggaaat ggttgtatct gcagtttttg aagatggaag
     6241 ggagcctgca atacttatcc ttgatcatat taccgatgta cgtaactttg gaagcattgc
     6301 acgtactgcc gaatgtgctg gttttgatgc tattgccatc cctgacaaag gggcagcgca
     6361 gataaattca gatgctatca agacttcttc aggtgcatta cacaatatta atatatgtag
     6421 ggtgacaaat cttactaatg ctgttaagta tcttaaagat tgtgggttga aagtatttgc
     6481 tgcaactgaa aaagctaaac aaccatatta taaaaccaat atgaaaggcc cggtagcaat
     6541 tgtcttggga gctgaagatg ccggaatttc tcctgaaata ttacgatatg cagacgaact
     6601 ggttcaaata cctatgatag gcaaaacttc ttctctaaat gtatctgtag ctgctggtat
     6661 aattatatat gaggttgtaa aacagcgttt aagtgacttt taaaaggttc tctttttttt
     6721 aatgaaatag tttccctaaa tgtttagttt caccacgttg ttaatggaaa aacgtctccc
     6781 ccttcctttg
//
LOCUS       DAOSPF010000118         6565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_11820_length_6565_cov_7.177419, whole genome shotgun sequence.
ACCESSION   DAOSPF010000118 DAOSPF010000000
VERSION     DAOSPF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11820_length_6565_cov_7.177419"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..755)
                     /locus_tag="PK252_14230"
     CDS             complement(99..755)
                     /locus_tag="PK252_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT15911.1"
                     /translation="MKKVFVNILRVKKFKTAFLFLISCSTFPFVSSQSLLTPEEAVNI
                     ALQNNFGILVARNNADIAKINNSPGAAGMLPTISANASANLTNNNVNTELINNTQIVK
                     PSSQAVSKSAGLALSWVLFDGGKMFITKSKLNKLEDLGNTYYKDTIQQTVYNVMVAYY
                     NVVSQKQQLNSVNEAINFGNEQVKILQKSFDAGLTSKSSLLQAKIDLNVFKESAIATK
                     "
     gene            complement(762..3851)
                     /locus_tag="PK252_14235"
     CDS             complement(762..3851)
                     /locus_tag="PK252_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT15912.1"
                     /translation="MTFSDIALKRPVGSVVLSLIIVLFGLVGYNFLGVRLYPAIDPPV
                     IGVQTRYTGANAEIIESQITEPLEKALNGIEGVKSISSMSITGMSSISVKFEVGANLE
                     KAANDVRDKTSQAMRSLPQDIDAPPVVSKADANSDPIIIMSVQSSTMNALELGEYAEN
                     VLQEKLQTIPGVSSVGLFGQKRTAMRLWIDPNKMAAYNVTAQDVHNALLKENVELPGG
                     KIIGKATEVIVKVYGRLITEEDFNNLIIRQTNDQIVRFSDIGEAVLGPENEEVSVKLN
                     GSDGMSLALIPLPGANHIEIADEFYKRFEDIKKNLPEGLDVYIGYDRTVFVRQAVKDV
                     LETLAIALFLVVLIVFIFFREWKIALRPLIDIPVSLMGTFFIMYIAGFTVNVLTLLAV
                     VLATGLVVDDGIVVTENIFKKIEKGMDKWQAAFEGTREIFFAVISTSLTLAVVFVPVI
                     FLQGFTGRLFREFGVVVAGAVMISALVSLTLTPVLNVFLSSQKMKHSSFYKATEPFFT
                     SLESGYRKRLGSFMKRKLWPIWILVACLALIVVIGASLKSELAPLEDHSVIRANITTP
                     EGASFDYTSGVLDKLSAFVLDSVPEARLVFTRTGGSWSGSNTGFMNMFLWEPKDRKAS
                     QQEVYDRLIQSFKRFPEARIIPNQEPTISTSLASGSQLPVQFVLQNLDFKKIQTVLPK
                     FLDEARKSPVFSNVDANLKFNKPELNITIDRAKATNLGVNVRDVSSTLDYALSGRRYD
                     YFLRNGKQYFVIGQVDRSGRSKTADISSLYVRSNTGALIQLDNLVNIQENSNPPTLYH
                     FNRYKSATISASLAEGYTLGDGIDEMNNIGKKLLDDSFQTSLLGSSRDFAESSSNISF
                     ALILALVLIYLILAAQFESFRDPFIIMLTVPLAIAGAIVSLWLFGQTLNIFSEIGMIM
                     LIGIVTKNGILIVEFANQKRKTGLSKAEAAFEAAIARLRPILMTSLATIFGALPIALA
                     LGAGATSRISMGIVIVGGLLFALILTLFVIPSMYVLISGKGHKQSDDAVVPPSHSLL"
     gene            complement(3853..4893)
                     /locus_tag="PK252_14240"
     CDS             complement(3853..4893)
                     /locus_tag="PK252_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT15913.1"
                     /translation="MLYFKTFAIVAACFVLASCSNKGQEGKSAAKSNKVSYIEGYIVK
                     PSILEESVNVSGTVKPYEETVLMPDISGRVVAINFQEGSYVKKGTLLVQLFNDDLQAQ
                     LHKLQSTLDIAQQTEKRQAELMAVNGISKQSYDEAVLHVHSVKADIEVVQAQIRKTMI
                     VAPFDGYIGLRNVSVGTIVTPSTAIATIRQVDQLKLEFSLPGKYATMIKSGSKVKFNI
                     QGIDGQFEARVVATEEGVDLNTRNLKAKALVVSKNKNIVPGMYADVQIQIRNNTKALM
                     VPTQALIPQERNKKIIVSRQGKAAFLVVKTGIRQEGAIEVVEGINIGDTVVTSGILFV
                     KPGAVLNFAKVN"
     gene            complement(4991..5809)
                     /locus_tag="PK252_14245"
     CDS             complement(4991..5809)
                     /locus_tag="PK252_14245"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOT15914.1"
                     /translation="MKNSWLIILTLVFVSCESVIDLPIEEPGSQYVVNSILNPDSVVR
                     VYISKSVSLTDSAYFPDVNDATVILYENNTTIDTLKHHPGGEYRSLYKPKPLMQYSAK
                     VFTSDNVTLQALDSVPSLPEINSPALVRNDSGIILLNMLLEDSPVSGNIYLFRVFEKR
                     KNGKLYKVEFSIKNFERMLSMIDTSIVFTDSEFNGTRKKMEIYFSAPYAQQIIVRVSI
                     ISKAYYLYLLTWTQNHLANQISEYERVPNYCNIQHGLGIWCAESQKEFTISLMK"
     gene            complement(5806..>6565)
                     /locus_tag="PK252_14250"
     CDS             complement(5806..>6565)
                     /locus_tag="PK252_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT15915.1"
                     /translation="HMGSTGSPSFLTVDIWVPLSQYVKPQISDQVSFGATYARNTWEI
                     NSELFWKNFTGLIEKKIFVDEYFGVLDWRQNWQVCSGQAYGLELMAYKRTGRFNGWLS
                     YTLTFSDREYNSAFEQYHSPMSNIHKHLVHANANYVLSKKTRVSVGWTYGNGAMTTFP
                     KQKYQSAFNDMPFVYYVDGLNTYKMPAYHRLDLNVSFTKKKKYYRRTWNIGVYNVYNN
                     MNTNFVEIRKNKFVKTSYLGIIPSVSYVLEFGEQ"
BASE COUNT         1950 a         1423 c         1215 g         1977 t
ORIGIN      
        1 cctataaaaa gggttgacgg tattttctac cgttctttca aaaccacgaa aaccttcaaa
       61 atcagtgttt gtccgtactc agagtagtgc tataaggctc attttgtggc aattgcactt
      121 tctttgaaaa catttaagtc aatctttgcc tgcaaaagac tactttttga tgtcaaacct
      181 gcatcaaagc ttttttgtaa aatctttacc tgttcgttgc caaagttaat ggcttcatta
      241 acagagttaa gttgttgttt ttggctgaca acattgtaat atgccaccat cacgttgtaa
      301 accgtttgct gaatggtgtc tttataatac gtattgccca gatcttccag tttattcaac
      361 ttagatttgg taataaacat ctttccccca tcaaacaaaa cccagctaag agccagtcct
      421 gcacttttag atactgcctg gctactgggc ttgacaattt gtgtattgtt aatcaattcg
      481 gtatttacat tgttgttggt caggttagcc gaagcatttg ccgagattgt aggcagcatg
      541 ccggcagcgc ccggcgagtt gtttatcttg gcaatatcag cattgttgcg ggccaccaaa
      601 atcccgaaat tattttgcaa tgcaatattc acagcttctt caggtgtcaa taaagattgg
      661 ctgcttacaa aaggaaaagt actgcatgaa atcaaaaaca gaaacgctgt tttgaacttt
      721 tttacacgta atatattgac aaaaactttt ttcataccta tttacaataa tgaatgagat
      781 ggaggaacca cagcatcatc gctttgttta tggcctttgc ccgatatgag cacatacatg
      841 gaaggaatga caaacaatgt aaggataagg gcaaacagca accctccaac aatgactata
      901 cccatcgaaa tacggcttgt agccccggcc cccaaagcta aagctattgg caaagctcca
      961 aagatagtgg caagcgaagt catcaaaatt gggcgcaaac gggcaatggc agcttcaaat
     1021 gcagcttccg ccttgcttag tccggttttg cgcttttgat tggcaaattc gacaatcaaa
     1081 atcccgtttt tggtcacaat acctatcagc atgatcatgc ctatttctga aaatatattc
     1141 aacgtttggc caaacagcca cagactgact atagcgccgg caatagcaag tggaacggta
     1201 agcatgataa taaacgggtc gcggaagctc tcaaactgag ctgccagaat aaggtatatc
     1261 agcaccaggg caagtattaa cgcaaatgag atattggaac tactttcggc aaaatctctc
     1321 gaagaaccca gcagcgaggt ttgaaaagaa tcgtcaagca gttttttgcc aatattgttc
     1381 atttcatcaa ttccatctcc caaagtataa ccctcagcaa gtgatgccga aatggttgct
     1441 gatttatacc tgttaaaatg gtacaatgtt ggcgggttgc tattttcctg aatgttcacc
     1501 aggttgtcga gttggattaa tgctcccgta ttgctcctga cgtacaacga actaatatca
     1561 gcagttttgc tacgtcccga acggtccact tgtccaataa cgaaatattg cttgccattt
     1621 ctcaaaaaat agtcatatct tctgccgctt aacgcataat ccagggtact tgatacatct
     1681 ctcacgttaa ctccaaggtt agtggctttt gccctgtcaa tggtgatatt tagctctggc
     1741 ttgttgaact taaggttggc atcaacattt gaaaaaaccg ggctttttcg ggcttcatca
     1801 agaaattttg gcaacactgt ctgtatcttt ttaaaatcaa ggttttgcaa tacaaattgt
     1861 actggaagtt gcgaacctga agctaaagaa gtagatattg ttggctcttg gtttggaatg
     1921 atgcgggctt cgggaaacct tttgaaactt tggatgagcc tgtcgtacac ttcttgctga
     1981 gaagcctttc tgtctttagg ttcccaaagg aacatgttca taaaaccagt attggagccc
     2041 gaccagcttc ctccggtacg ggtaaataca agtcttgctt cagggacaga atcaagaaca
     2101 aatgcactta acttatctaa taccccgctg gtataatcga aacttgctcc ttcgggagtg
     2161 gtaatatttg ccctgatgac actatggtct tctaacgggg ccagctccga ttttaatgaa
     2221 gcccctatta ctacaattaa tgccaaacaa gctactaaaa tccatatagg ccacaatttg
     2281 cgtttcatga aactgcccag ccttttgcga tagccacttt cgaggctggt aaaaaatggt
     2341 tcagttgcct tataaaacga agaatgtttc attttttggc tactgagaaa aacgtttaaa
     2401 acaggggtca aagtcaatga tactaaagct gaaatcatta ctgctccggc cacaacaact
     2461 ccaaactcgc gaaatagcct tccggtaaag ccttgcaaaa agataaccgg gacaaacaca
     2521 acagccaaag ttagggaagt ggagatgacg gcaaaaaata tctcacgcgt gccttcaaaa
     2581 gcagcctgcc atttgtccat ccctttctca atttttttaa atatattttc ggttacaaca
     2641 ataccatcgt caacaacaag gccggtagca agcactacgg cgagcaaagt aagtacgttt
     2701 accgtaaacc ctgcgatata cataataaaa aatgtaccca tcaacgatac cgggatgtcg
     2761 ataagaggac gcaaagctat tttccattcc ctgaaaaaga tgaaaacgat aagcacaacc
     2821 aaaaacaggg ctatggccaa agtttccaac acatctttga ctgcctggcg cacaaagact
     2881 gtgcggtcgt aacctatgta aacatcaagc ccttcgggca ggttcttttt tatatcctca
     2941 aaccgtttgt aaaattcatc agcaatctca atatggttag ccccgggcaa aggtatcaat
     3001 gccagcgaca tgccatctga gccattaagt tttacgctca cttcttcatt ctcagggccc
     3061 aacactgctt ccccaatatc gctaaaacgc actatctgat cattggtttg ccgtataatc
     3121 aggttgttga aatcttcttc agtaatcaaa cgcccataaa ccttaacaat aacttcggta
     3181 gccttgccaa taatctttcc tccaggcagt tccacatttt cttttaacag ggcattatga
     3241 acgtcttgtg ctgtaacatt gtaagcagcc attttattgg ggtcaatcca caaacgcata
     3301 gcagttcgct tttgaccaaa taaacctaca gagctgaccc cggggatggt ttgtaacttc
     3361 tcctgcaaaa cattttcagc atattcgccc aattcaaggg cattcatcgt agagctttgt
     3421 accgacataa taatgatggg gtcagagttg gcgtccgctt ttgaaaccac aggcggagca
     3481 tcaatatcct ggggcaggct gcgcattgcc tgcgaagttt tgtcccggac atcatttgct
     3541 gctttctcaa ggttggcacc tacttcaaac ttaacgctaa tgctgctcat gccggtaatt
     3601 gacatggatg aaattgactt tacgccttcg ataccattga gggccttttc aagaggttcg
     3661 gttatctgcg actcaataat ttcggcatta gcgccggtat aacgtgtttg aaccccaatt
     3721 acaggagggt caatagccgg ataaagacgt accccaagaa agttgtagcc gactaaacca
     3781 aaaagaacaa tgattaaact gagcactacc gatcctaccg gacgttttag tgcaatatct
     3841 gaaaaggtca tattaattga ctttagcaaa atttagcact gcaccgggct ttacaaacag
     3901 tatccctgaa gttaccacag tatcgccgat gttaattcct tcaacaactt ctatcgcccc
     3961 ttcctgcctg attccggttt ttaccaccaa aaaagcggcc ttaccctgac gtgaaacaat
     4021 aatttttttg tttcgttcct gagggattaa agcttgtgta ggtaccatca atgctttagt
     4081 attgtttcta atttgtattt gaacatcggc atacatgcca ggaacaatat ttttgttttt
     4141 cgaaactact aacgctttag ctttaaggtt acgggtgttt aaatcaacgc cttcttcagt
     4201 ggcaactact cttgcttcaa actgtccatc aattccctgg atgttgaact tgacttttga
     4261 accgctttta atcattgtgg catatttgcc gggcaaacta aactccagct tgagttggtc
     4321 aacctggcgt atggtagcaa ttgcagtgga aggggttaca atagtcccaa cgcttacatt
     4381 gcgcaatcct atgtaaccat caaagggggc aacaatcatt gtctttcgta tttgggcttg
     4441 taccacctca atgtcagctt tcacagaatg cacgtgcaat acagcctcat catacgattg
     4501 tttgctaatg ccgttgacag ccataagctc tgcctgcctt ttttctgtct gctgggcaat
     4561 atctaaagta gattgtaatt tatgaagttg tgcttgcaaa tcatcattaa acagctgaac
     4621 cagcaatgtc ccttttttta cgtaagaacc ttcctgaaaa ttaatagcta caacacgtcc
     4681 actaatatca ggcattaaaa cggtttcctc ataaggttta actgttcccg acacatttac
     4741 actttcttcc aatattgaag gttttactat ataaccttcg atataactta ctttatttga
     4801 ttttgcagcc gatttgccct cctggccttt atttgaacat gaagctaaaa caaaacatgc
     4861 cgcaacaatg gcaaaagttt taaaataaag catgacaatc ttttttgaat cctaaaatag
     4921 ctttttaata taaacacgca aaggtagttt ataccaagtt gatatatgtt aaagtaaatt
     4981 aaaaaagtcc ctatttcatt agagaaatag taaattcctt ttgactttcg gcacaccaaa
     5041 taccaagccc atgctgaata ttgcaatagt tgggtactct ttcatactca gatatttggt
     5101 tggcgaggtg attttgtgtc caggtaagca aatacaggta atatgcttta cttattatac
     5161 ttaccctgac aataatctgt tgagcgtagg gggcactaaa gtatatctcc attttttttc
     5221 ttgtaccatt aaattctgaa tcagtaaaaa caatgctagt atctatcatg cttagcattc
     5281 gttcaaaatt cttgattgaa aactccacct tgtaaagctt tccgtttttt cttttttcaa
     5341 aaacacggaa taaataaata ttaccactga ccgggctatc ttctaacaac atatttaaaa
     5401 ggataatgcc actatcattg cgtactaatg caggagaatt aatttctggc aaagatggga
     5461 cactatctaa tgcctgtaat gtgacattgt ctgaggtaaa cacttttgca gaatactgca
     5521 ttagtggttt aggtttatat aaagacctgt attcccctcc ggggtgatgt tttagtgtat
     5581 caatggtagt gttgttttca tacaaaatta ctgttgcatc attaacatct gggaaatagg
     5641 cactgtctgt caatgaaacg ctcttgctta tgtaaaccct cactacactg tccggattga
     5701 gaatgctatt cacaacatac tgacttccgg gttcttcaat aggcaagtca ataacacttt
     5761 cgcatgaaac aaaaacaagg gttaatataa tcagccagct atttttcatt gttctccaaa
     5821 ttctaaaaca tacgataccg atggaattat tcccaaatat gatgtcttga caaatttatt
     5881 ttttctaatc tctacaaaat tagtgttcat gttgttgtaa acattataaa cacctatgtt
     5941 ccatgttctt ctgtaatatt tctttttctt ggtaaaactc acatttaggt ccaatcggtg
     6001 ataagcaggc attttgtatg tattaagacc atctacataa tacacaaaag gcatatcatt
     6061 aaaggccgac tgatatttct gttttggaaa tgtagtcatt gcgccgttac cataagtcca
     6121 acctaccgaa actctcgttt tttttgacaa aacatagttg gcatttgcat gaaccagatg
     6181 cttgtgtata ttggacattg gtgaatgata ttgctcaaat gccgaattat attctctatc
     6241 cgaaaaagtc aaagtgtaac tcagccaacc attaaagcgc ccggttcttt tgtaagccat
     6301 aagttccaat ccataagcct gaccagagca tacctgccag ttttgccgcc aatccaatac
     6361 cccgaaatat tcatctacaa atatcttctt ttctatcagc ccggtaaagt ttttccaaaa
     6421 cagttctgaa tttatctccc atgtattgcg ggcgtaggtt gcaccaaagc ttacctggtc
     6481 agatatttgg ggttttacat attgcgaaag gggtacccaa atatcaaccg taagaaaact
     6541 gggcgaacct gttgaaccca tgtgc
//
LOCUS       DAOSPF010000119         5997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_13364_length_5997_cov_6.755974, whole genome shotgun sequence.
ACCESSION   DAOSPF010000119 DAOSPF010000000
VERSION     DAOSPF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13364_length_5997_cov_6.755974"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK252_14255"
     CDS             <1..380
                     /locus_tag="PK252_14255"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOT15916.1"
                     /translation="ALKLPSQVKSNEGIKVQVTVLNSGTKEGDEVVQLYVRHPNVTLP
                     VPLYSLKGFKKVHLKAGERTNVTFNLTSKDLAILDKDNKWFVHYGDIEIYVGGSLPNK
                     DLLKEKKILMGTVSMSGDDFYLP"
     gene            complement(780..1154)
                     /locus_tag="PK252_14260"
     CDS             complement(780..1154)
                     /locus_tag="PK252_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15917.1"
                     /translation="MYDILVVDDEPLIREGLKVALEMEGYHAQTAADGKEAVKIVKMT
                     NPNILITDIIMPEKDGIEVICDVKRFNPNIKILAISGGGRISAQEHLKIAKQLGANSV
                     LAKPFSTEELMFEIKRMAEHAN"
     gene            complement(1173..3176)
                     /locus_tag="PK252_14265"
     CDS             complement(1173..3176)
                     /locus_tag="PK252_14265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15918.1"
                     /translation="MTFFLFQSNYLDLGIVILIILLLSFYSLMKYLACCFTPATIENK
                     QAVKSQQPFLFSKTTELTIEKTEVKDEYHWDELFQQLPVPVAIISQKGQVIKSNLAFN
                     SYFHLHSNEAIKSYSFLNDDNRDEESNQELEFMLKTKASFIIQSHWHINSSLHYIKET
                     YKPTPSGNFVVTIEDETETKKALRSAEIVEEKYNQLIEQLPVGIYRVLVTGEIVFINN
                     YLVKLLGYDSASEITGTSSEEVFKYPADYTIKKNKAIAKKKNSYVIEFQATGRNKQMM
                     WLKETATIFYDTSDEALLFDCIIEDITQKKLADLEVNRLYTAINQLSEGVIITDAEGT
                     ILYTNQSVEKMSQYSPKELVGKKMSIFKSEQQSEDFYKDLWKTIQSGKSWEGQIANRQ
                     KNGGLYIEYMLICPVKNENNDIENYIAIKKDISEKLKLEEQLRHSHNLQAIGTLTGGI
                     AHDFNNILMGMQIYTEVLLKKLDDKSQEHHLVSQIFDAEKRAKGLINQILTFGRSSVE
                     EIRPLQPHIIVKEATKLIQATYGSKIKIEQHIEDCGYINASPTHIFQIVMNLCTNAVQ
                     ALNENGLIETRLSLVSQINHPDGSIRHKGGSWICLSIKDNGSGIDDSIKDFIFEPFYT
                     TRIQGTGLGLSNVRDIVNNYEGEIFFDSIKGAGTTFYVYLPVK"
     gene            complement(3301..3816)
                     /locus_tag="PK252_14270"
     CDS             complement(3301..3816)
                     /locus_tag="PK252_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15919.1"
                     /translation="MHGIAFMEKEIKILVIDDDPFLQRLVEASLIKESFTMVFASSGE
                     EGLEKALEIVPDLILLDVIMPGIDGFEVCRQIRNSKTLQHIPVIILTALDDRDSKIQG
                     LEAGADDYIGKPFDKIEFKARVKTIIRLSRISKIEKLTELEAIIMEKDRIIEEQKKQI
                     AQLLSHANQSA"
     gene            4013..4618
                     /locus_tag="PK252_14275"
     CDS             4013..4618
                     /locus_tag="PK252_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT15920.1"
                     /translation="MSKIKLNVLGISYSHTQSGAYALVLAEEKGPRRIPIIVGSFEAQ
                     AIAIQLEGLNPPRPLTHDLFYNFATLFKIDILEVNIYKLEEGIFFSKLTCFNGKETIN
                     IDSRTSDAIALALRFSCPIYTTEDILKRSGITLEVENETKSGLSASKKKKEEVLNPEI
                     EELRSMSEDELKEALEEAINTENYERASKLRDEINRRNKQL"
     gene            4629..5060
                     /locus_tag="PK252_14280"
     CDS             4629..5060
                     /locus_tag="PK252_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT15921.1"
                     /translation="MIVVNDILKEKGHDFFFIYPQTLTIDALKFMAEKNVGALLVMEN
                     NVLCGIFSERDYARKVVLLGKSSVNTPVMDVMTSNLVVVKKETSINECMQLMTRQHVR
                     HLPVLEGEQVVGVVSIGDVVSRIIREQDNMIKQLEDYIYKG"
     gene            complement(5201..>5997)
                     /locus_tag="PK252_14285"
     CDS             complement(5201..>5997)
                     /locus_tag="PK252_14285"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOT15922.1"
                     /translation="YVLINELTKNCDAYFISSYFHKDKNSKDGRLKYGPLWDYDIAYQ
                     GARWQDGGIIEGWQFAQRDIGDPMYVKKIFKDTAVTHFYANRWKYLRKSFLADEKLFA
                     IIDSLAENIKDSRIRNYNVWPIIDKQYVWPVIATYTYEDDINVVKNFLTKRMKWIDNN
                     IDKIYYPLDVEELTYGKTMAYAYPNPFISRITVEFLGNGEEYSVILQSTDGRKIASKE
                     VSSLTNELVSVEFSNGTIQSLSPGMYFASIMQKGKLIQTVKIVKGK"
BASE COUNT         1767 a         1041 c         1109 g         2080 t
ORIGIN      
        1 cggcgttgaa gttgccttcg caggttaaat caaatgaagg tataaaagtt caggttacag
       61 tgttgaattc tggtacaaaa gaaggagatg aagtagttca gctatatgtc aggcatccga
      121 atgttacatt gcctgtccct ctttattcat tgaagggctt taaaaaggtt catcttaaag
      181 ccggcgaacg tactaatgtt acctttaatc taacttctaa agaccttgct atccttgata
      241 aagataacaa gtggtttgtg cattacggtg atattgagat ttatgttgga ggtagcctcc
      301 ctaataaaga tcttctgaaa gagaaaaaga tactgatggg taccgttagt atgtcaggag
      361 atgattttta tttaccttaa ccttagttcg acataggaaa aatgcttacg tatttgaaat
      421 ttacaggctt tagctaaatc ttcgtaactt ttgccttgaa gcaaaagttg caaaaaatca
      481 aggcttgcca taaattagct aaaaaagctt tatcgacggc tccgggcaac aactcgctat
      541 gctcaaacag ttgccctcgc ttcgccgata aagctttttt cttaacgcca tttccgtcag
      601 gccaaatgca gcaaagattc aatgaaaatg gcgacgcctt aaacagaaaa acttagtatt
      661 tattgctatt taatgctttt taaacaataa ttgacaatgt tattctttcg tctaactggc
      721 gttatcttag ggtgtaattg ataagaaaag ctttgcattt gcaagatgca aagcttttct
      781 taattggcat gttctgccat tcgctttatt tcaaacatca attcttctgt cgaaaagggt
      841 ttagccaata cactgtttgc gcccagttgt ttggcaattt taaggtgctc ttgtgcgcta
      901 atgcgccctc ctcctgatat agcaagtatt ttaatgtttg gattgaaacg ttttacatca
      961 caaataactt ctatgccatc tttttcgggc ataatgatat ctgtaatcaa aatattagga
     1021 ttagtcattt tgactatttt tacggcctct tttccatcag cggctgtttg agcatgataa
     1081 ccttccatct ccagcgccac tttgagccct tctctgatta acggctcatc atctacaact
     1141 aaaatatcat acatattatt aacacttaat gtttacttta ctggtaaata cacataaaac
     1201 gtggtccctg caccttttat tgaatcgaaa aaaatctccc cctcataatt gttcactata
     1261 tctcttacgt tggataaccc taatcctgtg ccttgaatac gggttgtata aaaaggttca
     1321 aagataaaat ctttaattga atcatctata ccgctaccgt tatctttgat agacaaacat
     1381 atccatgaac ctcctttgtg ccttatgctc ccatccggat ggttaatttg agagactaaa
     1441 gaaagccttg tttctatcag cccattttca tttaaagctt gaactgcatt ggtacacaaa
     1501 ttcatcacta tttggaaaat atgcgttgga ctggcattga tatacccgca gtcttcaata
     1561 tgttgttcta tttttatttt gctaccgtat gttgcctgaa tgagctttgt tgcctctttt
     1621 acaataatat ggggctgaag gggcctgatt tcttcaactg atgaacggcc gaatgttaat
     1681 atttgattga taagaccttt tgcccttttt tcggcatcga atatctggct taccaaatgg
     1741 tgctcctggc ttttatcatc gagttttttc aataatacct cggtatatat ttgcatgccc
     1801 ataagtatat tgttgaaatc gtgggctata ccaccggtaa gggtgccaat ggcttgcaaa
     1861 ttgtgcgaat gccgaagttg ctcttccagt ttaagttttt cagatatgtc tttttttatg
     1921 gcaatgtaat tctcaatgtc attattttcg ttttttaccg ggcatatcag catgtactcg
     1981 atgtacaaac ctccattttt ttgacggttg gcaatttgtc cttcccacga tttgcccgat
     2041 tgtatggttt tccataagtc tttataaaaa tcttcagatt gttgttcact tttgaaaatg
     2101 ctcatttttt tgcctaccaa ttctttgggt gagtattgcg acattttttc aacagattga
     2161 ttggtgtaaa gtattgtacc ttctgcatct gtgataataa cgccttcaga aagctggtta
     2221 atagctgtat ataggcggtt tacctcaagg tcggcaagtt ttttttgcgt gatatcttca
     2281 atgatgcaat cgaacaaaag cgcttcatcg gaagtatcgt aaaagattgt ggctgtttct
     2341 ttgagccaca tcatttgttt gtttctgcct gttgcttgaa actctataac gtagctgttc
     2401 tttttcttag ctatggcttt atttttcttt atagtataat cggccggata tttgaatact
     2461 tcttctgatg aagtacctgt tatttctgaa gctgaatcat atcccagcag tttaacaagg
     2521 taattgttaa tgaatacaat ttctccggtt acaagtaccc tgtatatccc aacgggcaat
     2581 tgttctatca actggttata cttttcttca acgatttctg ccgagcgcaa ggcttttttg
     2641 gtttcggttt cgtcttctat ggttacaaca aaattgcccg aaggcgtggg cttatatgtt
     2701 tctttgatgt aatgaaggct ggagtttata tgccaatggc tctgtatgat aaatgaagct
     2761 ttggttttaa gcataaactc aagctcctgg ttgctttctt catctctatt atcatcattg
     2821 agaaaactgt agctttttat agcttcattg gaatgaaggt gaaaatagga attaaaggct
     2881 aaatttgatt ttataacttg tcctttttgg cttataatgg caaccggaac aggtaattgc
     2941 tgaaacaatt catcccaatg gtattcgtct ttaacttcgg ttttttcaat ggttagttct
     3001 gttgtttttg aaaataaaaa tggctgttga ctttttacgg cctgtttatt ttctattgtg
     3061 gctggagtaa aacaacaggc cagatatttc attaaactat agaaagataa aagcagtatt
     3121 attaatataa caatacctaa atctaaatag ttagattgaa ataagaaaaa tgtcatttat
     3181 ttaaagttgg tttaatttgg cttcaaagat atggcatgta tttgtttatc aatcaaaata
     3241 ttagaatttt tgattaaaag gtacaaaagc ttgataaatg aaggaaatgt ttagataaaa
     3301 ctatgcgctt tggtttgcat gagaaaggag ctgggctatt tgctttttct gttcttcaat
     3361 aatacggtct ttttccatga taatggcttc aagctctgtc agtttttcaa tcttgctaat
     3421 gcggctcaat cggataatag tttttaccct ggctttaaac tctatcttgt caaagggctt
     3481 gccaatgtag tcatcagcgc ccgcctctaa tccttgtatt ttagaatctc ggtcatctaa
     3541 agctgtaagt ataataacag gtatgtgttg aagtgttttt gaattacgga tctgacggca
     3601 tacctcaaac ccgtcaatgc caggcataat tacgtcaagt aaaatcaggt cagggacaat
     3661 ttctaaggct ttctctaagc cttcttctcc acttgaagca aaaaccatgg tgaaactttc
     3721 ttttatcagg ctggcctcaa ccaaacgctg aagaaaaggg tcgtcatcga taactaatat
     3781 ttttatttct ttttccataa aagctatacc atgcattaac tctggagtat ttactttttt
     3841 actttttcaa atttagtaat aaatgtatta taaaggttat gtattgaaga ataattctct
     3901 catataaaaa tgtttttcga aagggaatag ttcaaaatgt aaatcgcata ttatatagat
     3961 tgtgaataat aattatattt gtgtataaaa ctgaattatt gtacaatctg atatgagcaa
     4021 aataaaactg aatgttttgg gtatttcata cagccataca caatccggtg cttatgcttt
     4081 ggttttggct gaagagaaag gaccaagacg gatccctatt attgttggtt catttgaggc
     4141 acaagccatc gctatccagt tggaagggct taaccctcca cgacctttaa cgcatgactt
     4201 attttataat tttgcaacac tgtttaaaat tgatattctg gaggttaata tttacaaact
     4261 tgaagagggt atattttttt ccaagcttac ttgttttaat ggaaaagaaa ctattaacat
     4321 tgactcacgg acttctgatg ctattgcttt agccttgcgt ttcagttgcc ctatttatac
     4381 taccgaagat atcttaaaac gttctgggat aaccctcgaa gtggaaaatg aaactaaatc
     4441 tggcttaagt gcttctaaga aaaagaaaga ggaggttttg aaccctgaga tagaagaatt
     4501 acgatcgatg agcgaagatg agttgaaaga agctttggaa gaggctatca atactgaaaa
     4561 ttatgaacgg gcttcaaaac ttagggatga aataaacagg cgaaataaac aattataaag
     4621 taagctctat gatagttgtt aatgatattt tgaaggaaaa aggacatgat ttttttttca
     4681 tataccccca aacgttaaca attgacgcct taaagtttat ggccgaaaaa aacgtagggg
     4741 ctttattggt catggaaaac aatgtattgt gtggcatttt ttcagaaagg gactatgccc
     4801 gtaaagttgt tttgcttggc aaatcatctg taaatacccc tgttatggat gttatgactt
     4861 caaacttggt ggtagttaag aaagaaactt caattaatga gtgtatgcaa ttgatgaccc
     4921 gtcaacatgt tagacatttg cctgtgttgg aaggtgaaca agtggtaggg gtggtttcta
     4981 ttggagatgt tgtaagccgc attatccgcg agcaggacaa catgattaaa cagttggaag
     5041 attatattta taaaggctga gaaaaaagaa ttacgaatta cgaattacga atattgttgt
     5101 tagaggacta atttacaaaa ggaagaaaat agaaagttga gcataaaaaa ggattgaact
     5161 aaaaaattca atcctttttt cttttattga aaagttttta ttatttgcct tttactatct
     5221 taacggtttg aataagtttg cctttctgca tgatagaagc aaaatacatg cccggagaaa
     5281 gtgattgaat agtgccattg ctgaattcaa cacttaccag ttcgttggtc aggctgctaa
     5341 cctctttgct ggcaattttg cggccatctg tcgactgaag tataaccgaa tattcttctc
     5401 cattacccag aaactcaaca gtaatcctgc taatgaatgg gttagggtag gcataagcca
     5461 ttgtttttcc ataagtaagc tcttcgacat ctaatgggta atatattttg tcaatattat
     5521 tgtcaatcca tttcattcgt tttgtcagaa aatttttgac cacattgata tcatcttcgt
     5581 aggtgtaggt ggcaataact ggccaaacgt attgtttgtc aataattggc caaacgttgt
     5641 aatttcttat tctcgaatct ttaatgtttt cggccagcga atcaataata gcaaatagtt
     5701 tttcatcagc caaaaagctt ttacgcaaat atttccatct gttagcgtaa aaatgggtta
     5761 ctgctgtgtc tttgaatatc tttttaacat acattgggtc gccaatatcc ctttgcgcaa
     5821 actgccaacc ttcgattata cctccatctt gccatctggc accctggtaa gctatgtcat
     5881 aatcccagag cgggccatat tttaaccttc catctttgga gtttttatct ttatgaaaat
     5941 aagagctaat aaagtatgcg tcacagtttt tggtcagttc gttgattaat acgtagt
//
LOCUS       DAOSPF010000120         5735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_14179_length_5735_cov_6.891549, whole genome shotgun sequence.
ACCESSION   DAOSPF010000120 DAOSPF010000000
VERSION     DAOSPF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14179_length_5735_cov_6.891549"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1504
                     /gene="ilvC"
                     /locus_tag="PK252_14290"
     CDS             29..1504
                     /gene="ilvC"
                     /locus_tag="PK252_14290"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT15923.1"
                     /translation="MANYFNSIPLRVQLEQLGKCDFMDQSEFADGVKKLLGKKIVILG
                     CGAQGLNQGLNLRDSGLDVSYALREDAIKQKRASYINASENKFKVGTFEELIPTADLV
                     INLTPDKQHTPVINKVIPLMKKGACLSYSHGFNIVEEGMQIRKDITVIMVAPKSPGSE
                     VRNEYVRGFGVPTLIAVHRENDPNGDGLEIAKAYCVAIGGHKAGVLHSSFVAEVKSDL
                     MGEQTILCGVLQTGSILCFNKMIEKGIDPGYASKLVQYGWETITEALKLGGITHMMDR
                     LDNPSKIKAFNLAEELKAIMRPLFEKHMDNIMSGEFSSTMMKDWANGDKQLLEWRAAT
                     GETAFEKTPAGSMEIKEQEYFDNGVLMVAFIKAGVELAFEVMVETGIKPESAYYESLH
                     ETPLIANTIARKKLYEMNRIISDTAEYGCYLFDHACRPLLADFMKKIDVNVIGKPFNP
                     NKDNHVDNLELIKVNEAIRNTEVEYIGYELRAAMEAMKPII"
     gene            1820..2284
                     /gene="nuoE"
                     /locus_tag="PK252_14295"
     CDS             1820..2284
                     /gene="nuoE"
                     /locus_tag="PK252_14295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT15924.1"
                     /translation="MKEIQDVVKNLTDKYGSKRESLLPILQGITEEQNYLSSEAMLEI
                     ARALDISAAEVYGTASFYSFLDTVPRGKYVIRVCRTIVCDMHGKKQIVKTLEDLLKIK
                     VGETTHNGKFTLLETNCLGWCHKGPCILVNDDIYTEITPAMVNEIINKYRNK"
     gene            2299..3483
                     /locus_tag="PK252_14300"
     CDS             2299..3483
                     /locus_tag="PK252_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT15925.1"
                     /translation="METKKIKRVDFIFSQDFKVSEVLFNTFKKTRDEILQEIIDSDLK
                     GRGGAGFPTGLKWKAAKEDKITEKYIICNADEGEPGTFKDRAILEEASQKVFGGMAIC
                     AKVIGAKKGWIYLRGEYKFLIPHLRKEMAIFHDMLHNELGFDFTIDIFTGSGAYVCGE
                     ETALLQSMEGKRGEPRNKPPYPTNIGLYNKPTVVNNVETLVLSMMVVKIGAEEFKKLG
                     IQDARGGKLFSVSGDTPKSGIHELEFGMLLSDFVEEFGDGDTKAVQVGGASGFCVPRK
                     KFDTTTIGYKGKLKGTSLPTGGSMMLFNSSRSMYNVLHNYLEFFAEESCGQCTPCRVG
                     TQQLLKGIEAIKKGEKSHEYLEALLSLSETMKLTSKCGLGQSVANSFSSIVENFREEM
                     IY"
     gene            3634..5424
                     /locus_tag="PK252_14305"
     CDS             3634..5424
                     /locus_tag="PK252_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT15926.1"
                     /translation="MSKKAMINITIDGVKLSMPEGSTILDAAKKENIRIPTLCYHEDL
                     CVAGNCRICVVEVDGWHTLAPSCSTPLMEGMVIHTTTPKVRKARKDIIALLVSEHDTQ
                     CTTCYRSTNCEFQALAAEYNVDNAVYLSVLKPNIELDRSSWSVEKDESKCVRCQRCVR
                     TCAELQKVNALAVTRKGRHMKISTFMDLPMNEVVCTNCGQCIIHCPTGALTERRYFDH
                     IWDAIGNPEKHVIINTAPSVRVALGEVLGFPVGTRVTGKMVTALKKIGFHSVLDTDFA
                     ADLTIVEEGHELLQRLKRAIVDKEDVALPMITSCSPGWVKFIEHMYPEYLQHLSTCKS
                     PQQMFGAMAKTYYAGKMGLDPKNIVSVAGMPCAAKKFEANRPEMKSSGFQDVDAGLTT
                     REIAMMIKQAGINFLELDDSKFDSIMGASTGAGVIFGATGGVMEAALRTVYEVVTGKD
                     VPFKNLNILPVRGLESIKEATIKIEGCKPEYSFLEGVELKVAIAHGLLNARIIMDQIV
                     AGKSPYHFIEVMACPGGCIGGGGQPIPTNNEIRQKRINAIYAEDEGMPIRKSHENPEI
                     KQIYKDFLKEPLGLKSHHLLHTKYTKRNRY"
BASE COUNT         1808 a         1060 c         1249 g         1618 t
ORIGIN      
        1 ttattaataa tcaataatta attaaaaaat ggcaaattat ttcaattcaa ttcctttacg
       61 tgttcaactc gaacaattag gaaaatgcga ttttatggac cagtcagagt ttgctgatgg
      121 agttaaaaag cttttaggta aaaaaatagt tattcttggc tgtggcgctc aaggcttaaa
      181 ccaaggctta aatctgcgcg atagcggcct ggatgtttct tatgctttgc gcgaagatgc
      241 tattaagcaa aaacgtgctt cgtatatcaa tgcttctgaa aataaattta aagtggggac
      301 ttttgaagaa cttatcccga ctgccgacct ggtaatcaac cttacaccgg acaaacaaca
      361 cactcctgtt atcaataagg tgataccatt gatgaaaaaa ggcgcttgct tgtcttattc
      421 gcacggtttc aatattgtag aagaagggat gcaaattcgt aaagatatta cggtaatcat
      481 ggttgctcct aaatctccag gctcagaagt tcgcaacgaa tatgtacgtg gttttggagt
      541 tcctaccctt attgctgttc accgcgaaaa cgaccccaat ggcgatggct tagaaattgc
      601 caaagcttat tgcgttgcta taggagggca caaggctggc gtgctacatt catcattcgt
      661 agctgaggta aaatcagacc ttatgggtga gcaaactatc ctttgcggtg tattgcaaac
      721 aggttctata ctttgcttca acaaaatgat tgaaaaaggc atagatcctg gttatgcttc
      781 aaaattggtt caatatggat gggaaactat caccgaagca ttgaaacttg gcggtattac
      841 ccacatgatg gaccgtttgg acaacccgtc aaaaattaaa gctttcaacc ttgctgaaga
      901 attaaaggcc atcatgcgcc cattgtttga aaaacacatg gataacatca tgtctggcga
      961 attttcaagc accatgatga aagattgggc caatggcgac aaacagcttc tcgaatggcg
     1021 tgcagctaca ggcgaaacag ctttcgaaaa aactcctgcc ggaagcatgg aaatcaaaga
     1081 acaagaatat tttgacaatg gcgtgttgat ggtagccttt atcaaagctg gtgttgaact
     1141 ggcttttgaa gtaatggtcg aaacaggtat caaacctgaa tcagcttact acgaatcatt
     1201 acacgaaact ccgcttattg ccaataccat tgcccgcaag aaattatacg agatgaaccg
     1261 cattatttca gatactgctg aatacggttg ctacctgttt gaccatgcat gcaggccgct
     1321 gttagccgat tttatgaaaa agatagacgt gaatgttatt ggcaaacctt tcaatcctaa
     1381 taaagacaac cacgttgaca accttgaact gattaaggtt aatgaagcta tccgcaacac
     1441 agaggttgaa tatattggtt acgaactccg tgctgctatg gaagccatga agccaatcat
     1501 ctaagcatta gagttgttct aagatacacc ataaaagctg ttctcaaaaa tgggaacagc
     1561 tttttttgtc ccccctcccc aaaaaaactg aagagtatag ccatcataca aaagtttatc
     1621 ccaaattaat cttcttctgt tgcctgcttt tattgcttct ataattgggg ctataattat
     1681 ctgtatttaa gctttttatt tcttccaaaa tttttgtaaa ttagtataag aaacagtttt
     1741 tcaaaaaaat tatacttaat agcctttatt atactctcag cttatttgca tttcttttta
     1801 tcccctaaac cgctaaataa tgaaagaaat acaagacgtt gtaaaaaacc ttaccgacaa
     1861 gtatggcagc aaaagagaaa gcttgttgcc cattcttcag ggcattaccg aagaacaaaa
     1921 ttatctttcc agcgaggcaa tgctcgaaat agcccgtgca ctcgatattt cagcagcaga
     1981 agtgtatggg acagcctctt tttattcgtt tctggatacc gttccccgag gtaaatatgt
     2041 cataagggtg tgtcgtacca ttgtttgcga tatgcatggc aaaaagcaga ttgtaaaaac
     2101 tttggaagac cttctgaaaa ttaaggtcgg agaaacaacc cataatggca agtttacttt
     2161 attagaaacc aactgccttg gctggtgtca caaaggcccg tgtattttgg taaatgacga
     2221 tatttatacc gagataaccc cggctatggt caatgaaatc attaacaaat accggaataa
     2281 ataaaagagg tcagccgtat ggaaacaaaa aaaataaaaa gggttgattt tatctttagc
     2341 caggatttca aagtttcgga ggtacttttt aatactttta aaaagacaag agatgaaatt
     2401 ctgcaggaaa tcattgattc ggatttgaaa ggacgtggag gggcaggttt cccaacagga
     2461 ttgaaatgga aggcagctaa agaagataaa ataacggaga aatacattat ttgtaatgct
     2521 gacgaaggag agccagggac cttcaaagac agggccatac tggaagaagc atcgcaaaag
     2581 gtatttggcg gtatggctat ttgtgccaag gtgattggtg caaaaaaagg ctggatatac
     2641 ctcaggggtg aatataaatt cctgattcct catttaagaa aagaaatggc catttttcat
     2701 gatatgttgc acaacgagct tggctttgat tttactattg atattttcac tggcagcgga
     2761 gcttatgtgt gtggcgaaga aacagcactt ctgcaatcaa tggaaggtaa acgcggagaa
     2821 cctcggaaca aaccaccata ccccaccaat attgggcttt acaataaacc tacagtggtc
     2881 aataacgtag aaacgctcgt tttatctatg atggtagtga aaattggggc tgaagagttt
     2941 aaaaagcttg gtatccagga tgcccgtgga ggaaagcttt tttcagtttc gggagataca
     3001 cccaaaagtg gcatacatga actggagttt ggtatgttgc tttcagattt tgtagaagaa
     3061 tttggcgatg gcgatactaa agctgttcag gtgggcggtg catctggttt ttgtgtgcct
     3121 cgcaaaaagt ttgatactac taccataggc tacaaaggaa aactaaaagg aacatcactt
     3181 cctacgggtg gttctatgat gttgtttaac agttcccgct cgatgtataa tgttttacat
     3241 aattatctcg aattttttgc cgaagaatcc tgcgggcaat gcacaccttg tcgtgtaggc
     3301 acacagcaac tgctaaaagg catagaagcc attaaaaaag gcgaaaaatc gcatgaatat
     3361 cttgaagccc ttttgagcct ttcggaaacg atgaaactta cttctaaatg cggattaggc
     3421 caatcggtag caaactcttt ttcttctatt gttgaaaatt tcagggaaga gatgatttat
     3481 taaaaccacc ccaacctccc gcctgaggcg ggacaagctc tccttgtaaa ggagtgggaa
     3541 tgaagctttt acaaaagaaa gttgtaagaa gtggatttta taatattaaa tgctgaaagt
     3601 gtataagttt ttaaaccaat taaacttatt gaaatgtcta agaaagcaat gataaatatt
     3661 actatagatg gcgtaaaact cagcatgcct gagggtagta caattttgga tgcagctaaa
     3721 aaagaaaata tcaggatccc gacgttatgc tatcacgaag acctttgtgt tgccggtaac
     3781 tgccgcattt gtgttgtaga ggtcgatgga tggcatactt tagcgccttc gtgttctact
     3841 cccttgatgg aaggaatggt tattcatact accactccta aagtcagaaa agcacgtaaa
     3901 gatatcattg cattacttgt ttcggagcat gatacgcagt gcacaacatg ctatcgtagt
     3961 acaaattgcg aatttcaagc tctggcagca gaatataatg tggacaatgc tgtttatctc
     4021 agcgtgctga aacctaatat tgaactcgac cgttcttctt ggtctgtaga aaaagatgaa
     4081 agcaaatgcg tacgctgtca gcgttgtgta aggacttgtg ccgaattgca gaaagtgaac
     4141 gctttggctg taacccgcaa agggcggcac atgaaaattt ctacgttcat ggatttgcct
     4201 atgaatgagg tagtttgtac caattgcggg caatgtatca tccattgtcc taccggggca
     4261 ctgactgaac gccgctattt tgaccatatt tgggatgcca ttggcaatcc tgaaaagcat
     4321 gttatcatca atactgcacc atcggtgcgt gttgctctgg gcgaggtgct cggttttccg
     4381 gttgggacaa gggttacagg taagatggtt actgcactta aaaaaatagg cttccattct
     4441 gtgctggata cagactttgc agccgacctg accattgtag aagaaggaca tgagctattg
     4501 caaaggctca aacgtgctat tgtggataaa gaagacgtcg ccctgcccat gattacctct
     4561 tgttcgcccg gctgggtgaa atttatagag cacatgtacc ccgaatattt gcagcatctg
     4621 agtacctgca aatcgccaca gcaaatgttt ggggcaatgg caaaaactta ttatgccggg
     4681 aaaatggggc ttgacccgaa aaatattgtt agtgtggccg gaatgccctg tgctgcaaaa
     4741 aaatttgaag ccaacaggcc tgaaatgaaa agcagtggct ttcaggatgt tgatgcaggc
     4801 cttactaccc gtgagatagc catgatgata aagcaagcgg gcattaattt tcttgaactt
     4861 gatgatagta agtttgacag tattatgggg gcttctaccg gggcaggggt aatatttggc
     4921 gcaacaggag gcgttatgga agctgcactc aggactgttt acgaagtggt aaccggcaaa
     4981 gatgtgccat ttaagaattt gaacatttta cctgtccgag gtttagaatc tatcaaggaa
     5041 gcaaccataa aaatcgaagg atgtaaacct gagtatagct ttctcgaagg ggttgaactc
     5101 aaagtagcta ttgctcatgg gttgcttaat gcccgtatta tcatggacca gattgttgct
     5161 ggtaaatctc cttatcattt tattgaagtg atggcttgtc cgggagggtg tataggaggt
     5221 ggcggacaac ctatcccaac caataatgaa atacgtcaga aacgtataaa tgctatatat
     5281 gctgaagatg aagggatgcc tatccgaaaa tcgcacgaaa accctgaaat aaagcagata
     5341 tataaagatt ttttgaaaga gccacttgga cttaaatcgc atcatttgct acacaccaaa
     5401 tataccaaac gaaacaggta ttgaaaaata tatccacttg taccaatcat aagtggcatt
     5461 agttagtgtt agttttgtta gttagtagaa aagaggctgt ataaaaagac caaaaggtcg
     5521 taagtatgca gcctcttaat ttagcaaaag ctcatgtatg ttcagttatc ggtcggtgac
     5581 cgcataaata ttgtcccata ttaatcatta aaaagctttt taaagccgaa ctgtatgatt
     5641 tagctgggtt aatcctcccg ttataaacga gacaagctga tttagaaatt gtttgaaaaa
     5701 ttttattaaa ctattgaatt acaatttttt aatcg
//
LOCUS       DAOSPF010000121         5511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_14889_length_5511_cov_5.800587, whole genome shotgun sequence.
ACCESSION   DAOSPF010000121 DAOSPF010000000
VERSION     DAOSPF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14889_length_5511_cov_5.800587"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..941)
                     /locus_tag="PK252_14310"
     CDS             complement(54..941)
                     /locus_tag="PK252_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT15927.1"
                     /translation="MKTLKILFVFVFVILGSSQAQEIVNLYSDKVPNAKVSGIKETYN
                     SGMYRGVITPTLQVFLPEKGKGTGAAVVICPGGGYYVVVYDGEGVLTAKELAKNGIAA
                     FVLKYRLPDDSLEMDKTIAPLQDAQQAIKVVRENAANWGVDVHKVGIMGFSAGGHLAS
                     TVATHFDKPVIENTNNTNLRPDFQILVYPVISMQDSLTHRDSRNCLLGKNPSKQMIDL
                     YSNELQVTANTPPAYLTHAGDDKLVDVDNSIVYFEKLRHLKVPVEMHIYQKGDHGFIF
                     KHQGWMEPLFRWMKDSGIN"
     gene            complement(938..1861)
                     /locus_tag="PK252_14315"
     CDS             complement(938..1861)
                     /locus_tag="PK252_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT15928.1"
                     /translation="MKKFSIILALLFSVSCFGQKYSKCWKDVNYAGDTMGYHNLDIYL
                     PEVQKPSYPVVITIYGSAWLSNKSKGADLNTLGKVLLEAGFAVVMPNHRSSFDAKFPA
                     QVQDIKAAIRFIRANAAKYQVDTAFVGITGSSSGGNLAAMAGTTRNVKHYTQGGISID
                     IEGNVGNNTASSSSVDAVVDWFGPTNMLVMDSCGGTDFIHNDARSPASLYIGGPIQEN
                     KEKTLLASPMTYIDPSDPPFLIFHGEKDRVVPYCQSQLLYAALQKANVPSQFFLVPGG
                     QHGPEVHVDKYMKMMADFFVAESAKHKAVKK"
     gene            complement(1873..3360)
                     /locus_tag="PK252_14320"
     CDS             complement(1873..3360)
                     /locus_tag="PK252_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOT15929.1"
                     /translation="MRTNYFLKTVLTGIVALLFVPFLKGINTGTVTTITYYSTTQKVD
                     CKMNVYVPYGYDQEADTVNSYPVFYLIHGGGENYTHWVNSGNAKGTLDEFIAAGKAVP
                     MILVMPDAKNLAADVFAKELINDIIPYIEKNYRVKADKDHRGVGGLSWGGLQAMEAGI
                     YHYEMFGYLSVLSSGWFTNETATYNRAKAFLSAHGKDMEKSIRYLYFSEGTSTDIAYN
                     NGMATLKVLRDSGLTVHYWEHPGGHQWSAWREDFRSFAPFLFRDTATKYVSLAFQGGI
                     ITNSTIMTHLDSVIAEPVKPVRYGYTFAGWYKDAACLDSFDFAHDTIKKNITLYAKWS
                     INSYKISFNSNGGSAINDTIVANYNTLITAPIAPVKSGYVFDGWYTNGAFTKKWDFSK
                     DRVPNTDITLVAKWVDIASVSVDKVNMMKIAVFPNPVKSVLQINNLHSDASVAIFGID
                     GKLMVTEKLKAPNGTINVEALPEGFYKVKISNASFEMNQNIVVKR"
     gene            complement(3398..4732)
                     /locus_tag="PK252_14325"
     CDS             complement(3398..4732)
                     /locus_tag="PK252_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT15930.1"
                     /translation="MKRICINLLFVLSFTAINAQNPIVRDQFTADPSARVFGDKVYVY
                     PSHDILAKDGQGRPGWFCMADYHVFSTSNLTDWTDHGVIVSQNKVKWVDSAAYAMWAP
                     DCIERNGKYYFYFPAIKKDTFDGRGFGIGVAVSDSPYGPFVPEEKPIKKVHGIDPNVF
                     IDKDGQAYLYWSMRNIYVAKLKDNMLEIEGEPQVIANLPENGLKEGPWLFERNGIYYM
                     TFPHVENKTERLEYAMGKSPMGPFSMTGVVMDESESGCWTNHHSFINFKGQWYLFYHN
                     NDLSPKFDKNRSIRVDSLFFNEDGTIRKVIPTFRGVGLTSASSEIQIDRYSAISKSGV
                     SVTLLDTANTFKGWKTVFENKDSWIQYNSVDFGKEKPKIIVIKALSEKGGDLLVKTSN
                     AGEKIIAKVKIPANKDWIEIKLPVSLSVKGVHNIVVSQVGDNPVEVDWISFK"
     gene            complement(4768..>5511)
                     /locus_tag="PK252_14330"
     CDS             complement(4768..>5511)
                     /locus_tag="PK252_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT15931.1"
                     /translation="RTKEGFVLRFYDQYTNKTIEEKLTSGKISLRAWGNYEKDIARFS
                     YSIDGNTFANIGDSVRLPYQLKTFQGSRYALFAYNTTGKEGGYADFEDFKLVEPLADR
                     SKNIPAGKVITLVNMANHDAVWANPHGMLHSVPVDSKEFEGKGCQFRVHDRGKGRVAL
                     EALSGAGFLTVVGIGISGDVRLLKEESEGSLFQWQDMLHNQCMLLSLKTNRYVGLTPG
                     TGEPYAADRPGALPNRKDGTVLVWKIVNE"
BASE COUNT         1630 a         1212 c         1019 g         1650 t
ORIGIN      
        1 atggttaatg gttaatggtt aatggttaat ggttaattcg taatttttaa tttttaattg
       61 atccccgagt ctttcatcca tctgaacaat ggctccatcc aaccttggtg tttgaagatg
      121 aaaccgtggt cacctttttg ataaatgtgc atttctacag gtactttgag atgccttagc
      181 ttttcaaaat agacaatact gttgtccaca tctaccagct tatcatcacc tgcatgagtg
      241 aggtatgcag gaggggtatt ggcagtcacc tgcaactcat tagagtataa atctatcatt
      301 tgcttcgaag gatttttacc cagcaaacaa ttccttgaat cgcgatgcgt caggctgtct
      361 tgcatgctta tcacagggta aaccaatatc tggaaatcag ggcgcaaatt ggtattattg
      421 gtattttcaa ttaccggttt gtcaaaatga gttgccacgg tagatgctaa atgtccacct
      481 gccgaaaaac ccataatacc taccttatga acatctacac cccaatttgc agcattttct
      541 ctaactactt ttatggcttg ctgggcatcc tgcaaaggag caatggtttt atccatttct
      601 aacgagtcat ccgggagcct gtatttgagt acaaaggctg caatgccatt tttggcaagt
      661 tctttggctg tcaatacccc ttcgccatca taaacaacaa catagtagcc acctccggga
      721 cagataacca cagcagcccc tgtccctttg cctttttcgg gcagaaatac ctgaagagta
      781 ggggtaatca ctcctcggta catacctgaa ttatatgttt cttttattcc tgaaaccttt
      841 gcattaggca ctttatctga gtacaagttt actatttcct gagcctgaga agaacccaag
      901 attacaaaga caaagacgaa taatattttc aacgttttca ttttttaaca gctttatgtt
      961 tagcagattc tgctacaaaa aagtcggcca tcattttcat atacttatca acatgcacct
     1021 ccggaccatg ctgtccgcca ggcacaagaa aaaactggct gggcacatta gctttttgca
     1081 atgctgcata aagcaactgg ctttgacaat aaggcactac gcggtctttt tcgccatgaa
     1141 agataagaaa tggagggtcg cttgggtcga tatacgtcat aggactggca agcaaagtct
     1201 tttccttatt ttcctgaata gggccaccaa tataaagcga tgcaggcgac ctggcatcgt
     1261 tgtggataaa gtcagtacca ccgcatgaat ccattaccag catattggtt gggccaaacc
     1321 agtcaacaac tgcatcaaca gagctactgg aagcggtatt attgccaaca tttccttcaa
     1381 tgtctataga tatgccgccc tgggtataat gtttcacatt gcgggttgtc cctgccatag
     1441 cagcaaggtt tccgccggat gagctaccag ttatgcctac aaacgcagta tctacctgat
     1501 atttggcagc attggcacgg ataaagcgga tagcggcttt tatatcctgc acctgggcag
     1561 ggaatttagc atcaaagctc gaacgatggt tgggcattac tactgcaaaa ccagcctcga
     1621 gtaacacttt gcctaaagta tttaagtcgg ctcctttaga tttattagaa agccaggcac
     1681 ttccataaat cgtgattact accggataag atggtttctg tacttcaggc agataaatat
     1741 cgaggttatg ataccccatt gtatcgccgg cataattaac atctttccag catttggaat
     1801 atttctgccc aaaacaggaa acagaaaaca atagcgcaag aatgattgaa aatttcttca
     1861 ttttatttta tatcaacgtt ttacaacaat attttgattc atttcaaaag aagcattgct
     1921 tatttttacc ttgtaaaagc cttcaggcaa ggcttcaaca tttatggtac cattgggggc
     1981 tttgagtttt tcagtgacca ttagctttcc atctatacca aagatagcaa cagaagcatc
     2041 tgaatgcaga ttatttattt gtagcactga tttgacaggg tttgggaaca cagcaatttt
     2101 catcatattt actttgtcaa cagatacaga agcgatatct acccatttag ctacaagtgt
     2161 aatgtctgta ttaggcactc tgtctttgct aaaatcccat tttttggtaa atgccccgtt
     2221 tgtgtaccag ccatcaaaca catatccaga ttttacagga gcaataggag ctgtaattaa
     2281 ggtattatag ttggcaacaa tagtatcatt aatagcactg cccccgtttg agttaaaact
     2341 aattttataa gaattgatac tccatttggc atatagtgta atgtttttct tgatggtatc
     2401 atgagcaaaa tcaaaactat caaggcatgc agcatctttg taccatccgg caaaagtata
     2461 gccatatctt acaggtttaa cgggctctgc tataaccgag tcgaggtggg tcatgatagt
     2521 agagttagta attattccac cctgaaatgc aagagaaaca tatttagttg ctgtatcgcg
     2581 gaaaagaaaa ggcgcaaatg acctgaaatc ttctcgccag gccgaccatt ggtgaccacc
     2641 aggatgttcc caataatgga ctgttaaacc agaatctcta agcactttga gggttgccat
     2701 accattgttg tatgcaatat cagtagatgt accctcactg aaatacaaat aacggatact
     2761 tttttccatg tctttgccat gtgctgacaa aaaggccttt gccctgttat aggtagctgt
     2821 ttcgtttgta aaccacccgg agctgaggac agacaaataa ccaaacattt cataatgata
     2881 aataccagct tccatagctt gcaatccacc ccaggaaagc cctcctaccc cacgatggtc
     2941 tttgtcagct tttaccctat agtttttttc tatgtatgga ataatatcgt taataagttc
     3001 cttagcaaaa acgtcagcag caagattttt cgcatcaggc atcaccagta tcattggcac
     3061 ggcttttcct gcagcaataa attcatcgag ggtacctttg gcattccccg aattgaccca
     3121 atgagtataa ttttcgcccc caccatggat taagtaaaat acaggataag aatttacagt
     3181 atcagcttcc tgatcgtacc cataaggcac ataaacattc attttacagt caactttttg
     3241 tgtagttgaa tagtaagtta ttgttgttac agttcctgtg tttatccctt ttaaaaatgg
     3301 gacaaacagc aaagcaacaa tacctgttag cactgttttc aaaaaataat tagttctcat
     3361 agcttatcag ttatatagtt aggtttcttt aaaaaaatta tttaaaactt atccaatcca
     3421 cttcaacggg attatctccg acttgtgaaa caacaatatt gtgaacacct ttgacagata
     3481 gcgatacagg caactttatc tcaatccagt ctttatttgc cggaattttg actttagcaa
     3541 ttatcttttc gccggcatta ctggttttta ccagcaagtc ccctcctttt tctgacaaag
     3601 ctttaatcac tattatcttg ggtttttctt ttccaaagtc aacactattg tattgaatcc
     3661 aggaatcttt attttcaaaa acagttttcc agcctttgaa agtattggca gtatcaagta
     3721 aagtaacaga tactccactt ttgcttatcg cactatacct gtcgatttga atttctgacg
     3781 aagcagaagt aaggcccact ccacgaaatg tagggattac cttcctgata gtcccgtctt
     3841 cattaaagaa aaggctatct acccttattg acctgttttt gtcaaactta ggggacaaat
     3901 cgttgttgtg ataaaacaaa taccactggc ctttaaagtt gataaaagaa tgatggttgg
     3961 tccaacatcc agactcagac tcatccataa caacgcctgt cattgaaaaa ggccccatag
     4021 ggctcttgcc catagcatat tccaggcgtt cggttttatt ttcgacatgc gggaaggtca
     4081 tgtaataaat cccatttcgt tcgaacaacc aggggccttc tttcaggcca ttttcaggga
     4141 gattagctat aacctggggt tcgccctcta tttcaagcat gttatcctta agtttggcca
     4201 cataaatatt gcgcatagac caataaaggt aggcttgccc gtctttatcg ataaacacgt
     4261 tagggtcaat tccatgtact ttttttatag gtttttcttc agggacgaat ggcccataag
     4321 ggctatcaga cacagcaacg ccaataccaa agccccttcc gtcaaaggta tcttttttta
     4381 tggcagggaa atagaaataa tacttaccat tacgctctat acaatcaggt gcccacatgg
     4441 cataagcagc agaatcaacc catttcactt tgttctggct tacaataacg ccatgatctg
     4501 tccaatcagt gagatttgaa gtcgaaaata catgataatc ggccatacag aaccatccgg
     4561 gacggccttg tccatcttta gcaagtatat catgcgaagg atacacgtaa accttgtcgc
     4621 caaaaaccct tgccgaagga tcggccgtga attggtctct aacaataggg ttttgggcat
     4681 taattgctgt aaaagacagc acaaataata gatttataca aatcctcttc atctcaatcc
     4741 ctttaaaatc attattttaa actatactta ttcattaact attttccaga ccaacactgt
     4801 accatccttg cggttaggaa gagccccggg acgatcggct gcataaggct cgcctgttcc
     4861 gggtgttaag cctacataac ggttggtttt tagcgacaaa agcatacatt ggttgtgcaa
     4921 catgtcctgc cactggaaaa gactgccttc agattcttct ttaagcaacc ttacatcgcc
     4981 agaaatacca atcccgacaa cagtaagaaa accagcacca ctcaatgctt caagggcaac
     5041 acgtccttta cccctgtcgt gaaccctgaa ttgacaaccc ttaccttcaa attctttaga
     5101 atctacgggg acagaatgta acattccatg agggtttgcc cacacagcat catgattggc
     5161 catattgacc aacgtaatta ccttgcctgc agggatattt tttgaacgat cggccaaagg
     5221 ctcaacaagc ttgaaatctt caaaatcggc atagccacct tctttgcctg tagtgttata
     5281 agcaaaaagt gcataacgcg aaccctgaaa agtcttgagt tgataaggca aacgaaccga
     5341 atcgccaata tttgcaaagg tattcccatc aatgctgtaa ctaaaccggg caatatcttt
     5401 ttcataattg ccccaggctc gcaagctaat tttgcctgaa gtaagttttt cttcaatggt
     5461 tttattggta tattggtcat aaaaacgcaa cacaaaaccc tctttagtgc g
//
LOCUS       DAOSPF010000122         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_15672_length_5295_cov_8.751336, whole genome shotgun sequence.
ACCESSION   DAOSPF010000122 DAOSPF010000000
VERSION     DAOSPF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15672_length_5295_cov_8.751336"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1851
                     /locus_tag="PK252_14335"
     CDS             385..1851
                     /locus_tag="PK252_14335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15932.1"
                     /translation="MEDFTPVQEKKKESILYVDDDKVNLELFYGLLSDDYDVILESSP
                     SRAFDLLNSKKVKVIVSDQRMPEELGLSFLERVHQVYPDIIKIIYTAYLDHDAALQAV
                     NQGSIFRYLLKPWNSEQMRITLESAIREYDLKHENSILLNELKHKNEALENALNTISE
                     NEQKFYGIFMDSSDGITIVQDEQLIEANSALVSILKMSNAMFEKYPSIKELLKHKFEP
                     LWALLSRKNIEDNKTIAETTVMLEDGQKKHLELNSKIILYKNAEATLSIIRDITERKE
                     HERKIMEAIVNTQEEEQRRYAQELHDGLGPILSTLKMYVEWLANPANQQNKETITRQT
                     IVFIDEAINQAKSIANNLSPHILQRFGLVNTLETYCNDAVKPLGIECTISSNLTTRLP
                     QHIEVPLYRVLMECINNTLKHAEAKKIIIRLRKVNNGLQINYNDNGKGFDIAQTLNVG
                     KGMGLSNIRNRINLIDGQVKFISNPGIGTDIEIFVPEG"
     gene            complement(1864..2712)
                     /locus_tag="PK252_14340"
     CDS             complement(1864..2712)
                     /locus_tag="PK252_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT15933.1"
                     /translation="MKPSDKDSKKIIVPWDFSDVSYYALEHAERIARSLDKQIELLHI
                     VAKEELMEEKEAELNKVAELAFTKYSIKPAVKILCGSIFTTISNYASDTSANMVIMGT
                     HGIKGFQKLTGSYALKVLLGSKVPFIVVQDRPRTDEKLSNIVFPIDFRGENKEKLYWA
                     IYLGKYFNSKVHLFKYPVTDPSLQKKVNINLNFAVRFLIQNSLDYEIHTAPKGEFGKE
                     TMKFAQEINADLILIVTSKNVSFIDFIFGSREQYIIANSARIPVMVVNPRSSFAGMGQ
                     FMFGNG"
     gene            complement(2767..>5295)
                     /locus_tag="PK252_14345"
     CDS             complement(2767..>5295)
                     /locus_tag="PK252_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15934.1"
                     /translation="ANIKNITGISANKLLNSIGHFYNHIHPEDIRILLDARHESLKSL
                     KILDVEIRYINENNDLRWLQLRMMYHRGQNGLTWWDGYVIDITERIRAQIAAREQENV
                     IRKIQEGIASKTGEELLTGILTKLSETLKADLIFIAQRTNNPEMLEAISINKNGGLKR
                     GIQYKIKNSPCEIVINGMTLSYKSGVAMHYPDSFLVTQLNAEGYIGIPLYDSQGHTIG
                     VMAAMYSNLVSDLSLAEQMLQIFSSRVGAELERLKFEQEIQLYSDVAFNMQIALNVFQ
                     IDEKNNPGTFTLIKANPAAANIIKMSSAEMVGKTFDEIYPQLKSYNIQQDLIKVISTG
                     KPYSNEEFRFFSPNGQLIFFTIKAFRVPNNCVGVLFEDITRRKHIEKDIIESQQRYRI
                     LFENSPNGIHLIATKGELSGCIASVNSKVSEMLGYSSKELIGMSPDNIMRDISQSEKL
                     AYTQRLMAGETVVFETHFYRKDNSSFPVEVTANVVSILDELYILSIDRDITEITNARK
                     RLQENIQFLTTLIETIPLPFYYKDKEGVYLGCNSVFEEYTGYKRKFIIGKKDKDIYPP
                     HYAEELQKADSKLLKSLKTQKYEGKIPFADASVKDVIFNKALFYNSEGSVIGIIGVMV
                     DITERKNIENKLLETVITTEEKERERFAGNLHDEVGPLLSSLKMYLSLLAETEDKGKK
                     DYIIPQVQTLIKEAIQTVREISNDLSPHILNNYGCIAAIESFIGLKKDFVHINFVQNI
                     ENKRFGQNKETIIYRIVKELLNNTIKHACATEVELKLVEEDGIIKLSYSDNGVGFDLN
                     SSLQKQTGSIGLLNIMSRVKTIEGTFKILTSKGKGFSFELTVPI"
BASE COUNT         1559 a         1023 c         1018 g         1695 t
ORIGIN      
        1 aatgcttcgc ctaaaggcga gggatttcac ccatcccaga attataaatt aaaaaacctc
       61 tgattggtaa atctattttg aggtttatgt aagctatcaa atacaaatat ttgagacttg
      121 tatcactcct atgcagcttt attactcaat acttctacca tacttttcta cttttatact
      181 tttatacttt tatactattg cccctaccgg gcttttcatt agctgagtta gcagcgatag
      241 tatggaagtc aatttgttac aaggataata aaagctccgt aagtgcggca catatttatt
      301 gcaggtttat atcggacaat aattaaaatt atcttgtaat aaccgcaaat attggttatt
      361 ttagagatga aatttaattt tttcatggaa gattttactc ctgttcagga aaaaaagaaa
      421 gaaagcatcc tatatgtaga tgacgacaag gtaaaccttg agctttttta tggtttacta
      481 agcgatgatt atgatgtcat tcttgaatca tcgccttcgc gggcttttga tttattgaat
      541 tctaaaaaag taaaggtcat agtttcggac caacgcatgc ccgaagagct tgggctgtct
      601 tttttagaaa gggtgcacca ggtttacccc gatattatca agataatcta tactgcttat
      661 ctcgaccatg atgcagcttt gcaggctgta aaccagggaa gtattttccg ctacctgttg
      721 aagccttgga attctgagca gatgcgtatt accctcgaaa gcgccattcg tgagtatgac
      781 ctcaagcatg agaatagtat tcttctgaat gaactgaagc acaaaaacga ggcattggaa
      841 aatgctttaa acaccatcag tgaaaatgag cagaagtttt atggcatttt tatggatagc
      901 tctgacggca ttaccattgt gcaggatgaa caactgattg aagctaactc agcattggta
      961 agcattttaa aaatgtccaa tgccatgttt gagaaatatc cgtctattaa agaactttta
     1021 aaacataaat tcgagccttt atgggcactt ctttcacgca aaaacattga agacaataaa
     1081 accattgctg aaaccactgt gatgttagaa gatggccaga aaaaacacct cgaattaaac
     1141 agtaagatta tattgtataa aaatgctgaa gctacccttt ctattatccg tgatattacc
     1201 gaacgcaaag agcatgagcg caaaataatg gaagctattg tcaatacgca ggaagaagag
     1261 caacgccggt atgcccagga attacatgac ggtctggggc caattctttc tacactgaaa
     1321 atgtatgtcg aatggcttgc caaccctgcc aaccagcaaa acaaagagac cataactcgc
     1381 caaacgattg tttttattga tgaagccata aaccaggcaa aaagcatagc caacaatctt
     1441 agtcctcata ttttgcaacg ttttggtctg gtcaatactc tcgagaccta ttgcaatgac
     1501 gctgtaaagc ctttaggtat agaatgtacc atctcatcaa acctcaccac ccgcctcccc
     1561 caacatatcg aggtgccttt ataccgtgtt ttgatggaat gcattaacaa cactttgaaa
     1621 catgccgaag caaaaaaaat tatcatccgc ttgagaaaag taaacaacgg gcttcaaata
     1681 aactataacg acaatggtaa aggttttgat attgcccaaa ctttaaacgt aggcaaaggc
     1741 atgggattgt caaatatccg taaccgcatt aaccttatcg acgggcaggt aaaatttatc
     1801 tctaacccgg gcattgggac agatatcgaa atttttgtac ctgaaggata gtttttaatt
     1861 ttttcagccg ttgccaaaca taaactgtcc catgcctgcg aagcttgagc gtgggttgac
     1921 aaccattacc gggatgcgtg cactattggc tataatatat tgttcacgcg acccaaatat
     1981 aaaatcgata aaactaacgt ttttcgaggt aacgataaga ataaggtcgg cattgatttc
     2041 ctgggcaaat ttcatcgttt ctttgccaaa ttcgccttta ggggcagtat gtatctcgta
     2101 gtccaaacta ttttgaatca aaaagcgtac ggcaaaattg aggtttatat ttactttctt
     2161 ttgcagcgaa gggtcagtta cgggatattt aaacaaatga acttttgaat tgaaatattt
     2221 acctaaataa attgcccagt aaagtttttc tttattttct cccctgaaat caataggaaa
     2281 aacaatgtta gaaagctttt cgtcagtcct tggcctgtct tgtactacaa taaaaggtac
     2341 tttagagcca agcaaaactt ttagcgcata gctaccagta agtttctgaa aacctttaat
     2401 accatgtgtg cccataatca ccatgttagc actggtatct gaagcatagt tagatatggt
     2461 agtgaatatc gagccacaaa gaatttttac tgcaggtttg atgctgtatt tggtaaaagc
     2521 aagctcagct actttgttga gctctgcttc tttttcttcc atcaattcct cttttgcaac
     2581 aatatgaagg agttcaattt gtttgtccaa cgaacgagct attctttcag catgttcaag
     2641 agcataatag gacacatctg aaaaatccca cggtacgata atctttttgc tgtctttgtc
     2701 gcttggcttc atgatgatga tattttaact ttgaaaatta atgaaaatat tgcaggtatg
     2761 caaattttat attggaacgg taagttcaaa tgaaaaacct ttgccttttg aggtaagtat
     2821 tttgaacgtg ccttctattg tttttacacg gctcatgatg ttaagcagcc ctatactgcc
     2881 agtttgcttt tgcaggctcg aattaaggtc aaagcccacg ccgttgtcgc tatatgaaag
     2941 ctttattatt ccatcttctt cgaccagttt tagttctact tcggtagcac aagcatgctt
     3001 gatggtattg ttcaacaact cttttacaat acgatagatg atggtttctt tattttggcc
     3061 aaaacgttta ttttcaatat tttgtacaaa attaatatga acaaaatctt ttttcagccc
     3121 aatgaatgac tcaatagcag ctatgcaacc atagttgttg agaatatgag ggctaaggtc
     3181 gttagaaatt tccctaaccg tttgaatagc ctctttgatc aaagtctgta cctgagggat
     3241 gatgtagtct ttcttgcctt tatcttctgt ttcggcaagc aacgagaggt acatttttaa
     3301 actcgagagt aaagggccca cttcatcgtg aagattacct gcaaagcgct cgcgttcttt
     3361 ttcttcggta gtgataactg tttcgagcag tttgttttca atgtttttgc gttcggtaat
     3421 gtccaccata accccaataa taccaattac gctaccttca gagttataaa acaaagcttt
     3481 gttaaagata acatccttta ccgaggcatc agcaaaaggg atttttcctt catatttttg
     3541 agttttcagg ctcttcaaaa gtttgctgtc ggctttttga agttcttcgg cataatgcgg
     3601 aggatatatg tctttatcct ttttacctat gataaatttg cgtttgtagc ctgtgtattc
     3661 ttcaaatacc gagttacatc caagatacac cccttctttg tctttgtagt aaaaaggcaa
     3721 cgggatagtt tctatcaaag tggtcaaaaa ctggatgttt tcttgcaatc gtttgcgggc
     3781 attggttatt tcggtaatat cgcgatcaat gcttaagatg tatagctcat ctaatataga
     3841 aacaacattg gcagttactt caacaggaaa gctgctgttg tctttacgat aaaaatgcgt
     3901 ttcaaaaact acggtttcgc ctgccatcag tcgctgggtg taggctaact tttcagattg
     3961 cgaaatatcg cgcataatat tgtccggcga catacctatg agctctttac tgctatagcc
     4021 caacatttca gacactttag agttgacaga ggcaatacag ccactcagct caccttttgt
     4081 agcgataaga tggatgccgt tgggggagtt ctcaaacaaa atacggtaac gctgctggct
     4141 ttcaatgatg tccttttcta tgtgttttct gcgggtgata tcttcaaaca atacaccgac
     4201 gcaattgttg ggtaccctaa aagctttgat ggtaaaaaat atgagctgtc cattcggtga
     4261 aaaaaacctg aattcttcgt tgctataagg cttcccggtt gaaataacct taatcaggtc
     4321 ttgctggata ttataagact tcagttgagg atatatctcg tcaaaagttt ttccaaccat
     4381 ttcggccgaa gacattttta tgatgttggc agcagcaggg tttgctttaa taagagtaaa
     4441 ggttccgggg ttgttttttt cgtctatttg aaaaacattg agggcgatct gcatattaaa
     4501 agcaacgtcc gaatatagct gtatttcctg ctcaaatttc aatctttcga gctctgcccc
     4561 aacccttgac gaaaatatct ggagcatttg ttcggcaagg gacaagtcgc ttaccaggtt
     4621 tgaatacata gcagccatta cacctattgt gtgtccttgt gagtcataaa gggggatacc
     4681 aatatatcct tcggcgttta attgtgtaac aaggaacgaa tcgggataat gcatggcaac
     4741 accagacttg tatgacaatg tcatgccatt aatgacaatt tcgcagggag aattttttat
     4801 tttatattgt atgccacgtt ttagcccccc atttttattt atcgaaatag cttctaacat
     4861 ttcggggtta ttggtacgtt gggcgatgaa tattaaatct gcttttagtg tttcggaaag
     4921 ctttgtcaaa atgccagtca gcaactcttc gcctgttttg gaggcaatac cttcctgtat
     4981 tttcctaatc acgttttctt gttcgcgggc agctatctgg gccctgatac gttcagtaat
     5041 atcaataaca tagccatccc accaggtaag tccattttgt ccacggtgat acatcattcg
     5101 cagttgtaac cagcgcaggt cattattttc attgatatac ctaatttcta catcaagtat
     5161 tttcaggctt tttaggcttt catgccgggc atcaagcaat attcttatat cttcgggatg
     5221 gatatggtta taaaaatggc caatgctatt gagcagtttg ttggcactga tgccagtgat
     5281 gtttttgata ttggc
//
LOCUS       DAOSPF010000123         5017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_16864_length_5017_cov_7.463523, whole genome shotgun sequence.
ACCESSION   DAOSPF010000123 DAOSPF010000000
VERSION     DAOSPF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16864_length_5017_cov_7.463523"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1380)
                     /locus_tag="PK252_14350"
     CDS             complement(298..1380)
                     /locus_tag="PK252_14350"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT15935.1"
                     /translation="MQRRFFHTFDTLRFLAFFKVFLLHIPIAGFPIFSYLKAGGGIGV
                     VFFFVLSGFLISYIIFHEKETTGKFNLYYFYLRRILRIWPLYFLMILFAFATPYILSI
                     VKINSSPEGYEPAWWMSCLFLENYKMLFTGMEPNVSPLGVMWSLCIEEHFYLLWGLSF
                     LFIKLKHVPVFLGISVLASIIANGIFHYQNWQTIEISTNLIYFCAGAIPAYLLTTKKE
                     AFEEKINRIPIFLKALMLVLCLVFVIVSPHTSNDWRWLYEPIIFSVLFACLICIVIPA
                     ENYFKVSDSNILSRLGVWTYGLYLYHTIVINLFYQLSLRFQINLSQFLPAILFFMASL
                     VTTIVISAFSYYLFENRFLKLKKYFY"
     gene            complement(1384..2511)
                     /locus_tag="PK252_14355"
     CDS             complement(1384..2511)
                     /locus_tag="PK252_14355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT15936.1"
                     /translation="MKPICMVDLQTQYASIKEEIDTAIQEVVSSAQYIKGPQVKQFEE
                     AVAKYMGVKYAISCANGTDALIIALKSLDLPAGSEVIVPSFTFAATAEAAIYCGLIPV
                     FADIDPGLFTIDINHAEKLVTSKTKAIIPVHLFGQCADMTRILRLANKHKLYVIEDAA
                     QAMGAAHHFPCGTPHKGGTLGNIGCTSFFPSKNLGCFGDGGCIFTNDDIFAQKCRLIA
                     NHGMAVKYEHEIPGVNSRLDTIQAAILNVKLKYLDNYISARQKAASHYNEELKKVKGI
                     KLPEKVKTSTHTYNQYSIIVEKGNRDELKKYLEDKGIPTMIYYPKALHQQKAFVQYVI
                     DTKHLKNTEMICGKILSLPMHTELDEEQIKYIADCIIQFFK"
     gene            2587..2763
                     /locus_tag="PK252_14360"
     CDS             2587..2763
                     /locus_tag="PK252_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15937.1"
                     /translation="MPLSTLISYCGYVCSEYPRYIATQNNDVHELENLARLWFRLGLR
                     DRVVSAQEMRCIVH"
     gene            3065..4396
                     /locus_tag="PK252_14365"
     CDS             3065..4396
                     /locus_tag="PK252_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15938.1"
                     /translation="MKVKFTIFALILVATITDLAAQANKVTTPKYRRSSIYTIMIDDA
                     GLVKADTIKQAFLTAPVPDKYNDHNLAIKTFDPRTITLSPEEKKSKGDNAGSKFGKTI
                     LNNTTGGLADTTDVADLPLKIEKFLKANGIARGMVAKWFNRNEKGEFNMELIGERGQY
                     DATEMAANIAKKTARGTASLSDAGEELIGNTFVVVTRFKYVSKEEIAEAAKGMLSMVE
                     KYGGSTAASIAKVGSVAATVAAKGYVVQTTSYLYKLRWNDSIAAVFYQDYWVDANNFD
                     QKKKEAFDTTNLFTLDLIGTEKAWADVQSSIFTKKSEAELVRIATVQAIDAVIAKLQK
                     KYDVFKTKTPIYTTEPLTAKIGLKESLEKGDKFEVLEQILDEKTGKTVYKRVGTIKVD
                     GSQIWDNRYMAAEIQPVDTTAGVQKKPVIDRTLFKGASSKYYEGMLIRQIK"
     gene            complement(4467..4904)
                     /locus_tag="PK252_14370"
     CDS             complement(4467..4904)
                     /locus_tag="PK252_14370"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT15939.1"
                     /translation="MKAFKFLLLTSAIFLLACTQQPKLKNGVYEGRTQSKYKQEPFLG
                     VSKITVENNVISNIEFYIIDTTNNELFDEKYEKHFVGNNEEYVQQCRNDWAGVQRYPK
                     ELLQRQCLDSVDAVTGATWSYNMFHDATTIALEKAKANKKYVR"
BASE COUNT         1597 a          950 c          975 g         1495 t
ORIGIN      
        1 acagactttt tgcggctttc agaaagggtt ggtgctaaat accgacaaag tcgggggcat
       61 cctccaacag tgtttccctg ttttattatt aaaagctttg tatttattgc ctttttaaca
      121 ataattggca atgttattct tatatcgaat tcacgttagt ttacagactt caaaataacc
      181 tcgatgagcg cttccttata cctttgattt cctgattttt gtgcaagtgc ctgaaattgc
      241 attgattggg cacattgaaa gagtgggggt tgaaatattt aaaaggaata aagcttctta
      301 ataaaaatac tttttcaact tgagaaacct gttttcaaac aaataatacg aaaaggcact
      361 aataacaatg gttgttacca aagatgccat gaaaaataag atagcaggca aaaactgaga
      421 caggttgatc tgaaaacgta ggctcagctg ataaaatagg ttgataacaa ttgtatggta
      481 aaggtaaagg ccgtaagtcc acactcctaa tcggcttaaa atattgctat cacttacctt
      541 gaaataattt tcggcaggaa tgacaataca aataaggcat gcaaagagta ctgaaaaaat
      601 aataggttca taaagccatc tccagtcatt ggaggtatga ggtgatacta tgacaaaaac
      661 taaacagagt accagcatca atgcttttaa aaatattggg atacgattta ttttttcttc
      721 aaaagcctct tttttagttg taagcagata tgccggaata gccccagcac agaaataaat
      781 aaggtttgtt gatatttcta ttgtttgcca gttttgataa tgaaatattc cgttggctat
      841 aatagaagct aatacagata ttcccagaaa aaccgggaca tgtttcagct ttataaacag
      901 aaaagataag ccccacaaca gatagaaatg ttcttcgata cacaatgacc acattacacc
      961 taaaggagat acatttggct ccataccagt gaataacatt ttataattct caaggaaaag
     1021 gcaggacatc caccatgcag gctcgtaccc ttcgggcgaa gaattgattt tgacaatgga
     1081 aagaatgtaa ggcgtggcga atgcaaaaag tatcatgagg aaatacaaag gccatatacg
     1141 aagtatcctt cgcaggtaaa aataataaag gttaaatttc ccagtagttt ctttttcgtg
     1201 aaaaataata taagagataa gaaaaccgct gagtacaaag aaaaagacta cgccaatccc
     1261 gccaccagcc ttcaaataac taaatattgg gaacccggca atagggatat gcagcagaaa
     1321 aaccttaaag aatgccagga acctaagcgt atcaaaggta tgaaaaaacc gtctttgcat
     1381 ggcctattta aaaaattgaa taatgcaatc ggctatatac tttatctgct cttcatccag
     1441 ttcagtatgc ataggcaacg aaagtatctt accacaaatc atctcggtgt ttttcaggtg
     1501 ttttgtatct ataacatatt ggacaaaggc tttttgctgg tgcaaggctt tgggatagta
     1561 tatcatggtc gggattccct tgtcttcaag atactttttc agttcatcgc ggttaccttt
     1621 ttcaacaata atagaatatt gattgtaggt atgcgtgctg gtcttaactt tttcgggcag
     1681 ttttatgcct ttcacttttt taagttcttc attataatga gaagccgctt tttgacgagc
     1741 tgaaatatag ttgtcaaggt attttagctt cacattgaga atggcagcct gtatggtatc
     1801 aagccttgag ttgacacccg gtatttcatg ttcatatttt actgccattc cgtgattggc
     1861 aatcaatctg cacttttgtg cgaatatgtc atcgttggta aaaatgcaac cgccatcgcc
     1921 aaaacaacca aggtttttgg acgggaaaaa cgaagtgcaa ccaatgttcc caagtgtacc
     1981 tcctttgtgt ggtgtgccac atgggaaatg atgggcagcg cccatagctt gtgcagcatc
     2041 ttcaatgaca taaagtttat gtttattggc aagtctcaga atacgggtca tatcggcgca
     2101 ttggccaaac aaatgaacag gaataatggc ctttgtttta gatgtcacca gcttctcggc
     2161 atggttgatg tctatggtaa acagccctgg atcaatatcc gcaaaaacag gtattaaacc
     2221 acagtatatg gccgcctcag ccgttgcagc aaaagtaaac gacgggacaa tcacttctga
     2281 accagctggt aaatcaagtg atttgagggc aataatcaat gcatcggttc cattggcgca
     2341 tgatatggca tattttacgc ccatatattt ggccacagct tcttcaaact gctttacctg
     2401 cgggccttta atgtattggg cagacgaaac aacctcctgg atggcggtat ctatttcttc
     2461 tttgatgctg gcatattgcg tttgcaggtc aaccatgcag atgggcttca tattccttta
     2521 ttttttgaac caaaaataaa atatattaac gctaaataat tacttttagt ctaaaattca
     2581 ttaataatgc ccctttctac ccttatttca tattgtggct atgtatgttc ggaatacccg
     2641 cgttatattg ccacgcaaaa caacgatgtg catgaattgg aaaaccttgc caggctttgg
     2701 tttcggcttg gtcttcgcga ccgtgttgtt tctgcccaag agatgaggtg catagtgcac
     2761 taaagataaa gcctgcagat atcaggttaa tacctgtcaa aaccttcagg ggacaaccca
     2821 ttgttgtggt ttcccctgcg ataagatgaa agcaactctc gaaagaaccg atgctttggc
     2881 tataattatc atagaacggt gcaatgacca tcaatatcaa cagctaaaaa aagcattcct
     2941 gatgaaaagg gaagttatca tggctcaact aaattcttaa caaacaatgt cagatgaatt
     3001 tgttactttc tgaaaaaata ttaattttaa acattattat tcctaatctt aaatcttaaa
     3061 acatatgaaa gtaaaattta ccatttttgc cctgattttg gttgccacca ttaccgattt
     3121 agcagcccag gcaaataaag tgactacgcc aaagtaccgt cgtagttcta tttacaccat
     3181 catgattgac gatgccggcc ttgtaaaagc cgatactatc aaacaggctt ttttaacagc
     3241 ccctgtaccc gataaataca atgaccacaa tctggccata aagacttttg accctcgcac
     3301 tattactctt tctcctgaag aaaaaaaatc aaaaggcgac aatgcaggtt cgaaatttgg
     3361 aaaaacaatt ttaaataata ctaccggagg ccttgctgat acaactgatg tggccgattt
     3421 gcctttaaaa attgaaaaat tccttaaagc caatggtatt gccaggggta tggttgccaa
     3481 atggtttaac aggaatgaaa aaggcgagtt taatatggaa cttattggcg agcgcggaca
     3541 atacgatgct accgagatgg ctgccaatat tgccaaaaaa actgcccgtg gcacagcttc
     3601 attgtcagat gccggcgaag aactgattgg caacactttc gttgttgtga cccggttcaa
     3661 atacgttagc aaagaagaaa ttgctgaggc tgccaaaggc atgctttcaa tggtcgaaaa
     3721 atatggcggt tctactgctg cttctattgc aaaggttggt tctgttgctg ctactgttgc
     3781 tgctaaaggt tatgttgttc aaactacctc ttacctttac aaacttcgtt ggaatgattc
     3841 aatagccgct gttttctatc aagattattg ggtagatgcc aacaattttg accaaaagaa
     3901 aaaagaagct tttgatacta ccaatctttt tacactcgat ttgattggta ctgagaaagc
     3961 atgggctgat gttcagtctt ctatctttac caaaaaaagc gaagcagagt tagttcgtat
     4021 tgctactgtt caggctatcg atgctgttat tgccaaatta cagaaaaaat atgacgtttt
     4081 caaaacaaag acccctattt ataccacaga gccccttaca gctaagattg gtctgaaaga
     4141 aagccttgaa aaaggtgaca aatttgaagt acttgagcaa attttggacg aaaaaacagg
     4201 taaaacagtt tacaaacgcg taggtaccat taaggtggac ggatctcaaa tttgggacaa
     4261 ccgttatatg gctgccgaaa tacaacctgt tgacactacg gcaggggtcc agaaaaagcc
     4321 tgtgattgac cgtacattgt tcaaaggcgc cagcagcaag tattacgaag gcatgcttat
     4381 tcgacagatt aagtaattat tgacaatttg aaatagtaaa agcccgttag tgaaaactga
     4441 cgggcttttg ctatttctaa taaaaattac cttacatatt tcttattagc tttagctttt
     4501 tcaagtgcta tggttgtagc atcatgaaac atattgtacg accatgtagc gcctgttaca
     4561 gcatcaacac tatccagaca ttgcctttgc agcagctctt tcgggtatcg ttgtacgcct
     4621 gcccagtcgt tacggcattg ttgaacatat tcttcattat tcccaacgaa atgcttttca
     4681 tacttttcgt caaaaagctc attattggta gtatcgatga tataaaactc aatgttggaa
     4741 ataacgttgt tctcaacggt tattttagaa acccctaaaa aaggctcttg tttgtatttc
     4801 gattgcgtgc ggccttcgta aacaccgttt ttgagcttgg gttgttgtgt acatgccaat
     4861 aaaaatatgg cagatgtaag caagaggaat ttaaaagctt tcataagttt tgtttactat
     4921 attacaacaa aaatagcttt tacccttatt agtaaggttc aatacttgtc aagtttcaca
     4981 aggttgttta tgataactta attgcaaaga taaaact
//
LOCUS       DAOSPF010000124         4943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_17182_length_4943_cov_7.207242, whole genome shotgun sequence.
ACCESSION   DAOSPF010000124 DAOSPF010000000
VERSION     DAOSPF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17182_length_4943_cov_7.207242"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="PK252_14375"
     CDS             <1..1471
                     /locus_tag="PK252_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT15940.1"
                     /translation="LYVDTVNQTALTETAIRAMVKELDPHSAYLNIEEVKELNEPLQG
                     NFEGIGVSFNILNDTIFIVNTIAGGPSEKVGVMGGDRIIKIDGELVAGIKITNKTVFK
                     KLRGNKGTKVVISVLRKGSKNLIDFTITRDKIPIYSIDAAYMLDKETGYIKLDRFSQT
                     TNEEFAKALKKLQSQKMKNLILDLTGNGGGLLDEAAMLADQFLEKDKLLVYTKGRKTR
                     RQDFFSTSQGLFEKGKLVIMIDENSASASEILSGAIQDWDRGIIVGRRSFGKGLVQRP
                     LMYPDGSMLKLTIAHYYTPSGRDIQRPYKNGNSDEYYNDFINRYKHNELSNKDSIHFV
                     DSLKKLTLVLKRPVFGGGGIMPDIFVPIDTSMYSDYYRDLLRNGTLYKFVLNYIDTNR
                     IALKKQYPDFQKFNASYCAEQLFPAMIDFASKDNIKFNQQQFEQSKNAISKLLKAYIA
                     RDIWDSSEFFQVFNSDDPIIKEALKAIDQYDTIISPPKK"
     gene            1483..2088
                     /gene="pnuC"
                     /locus_tag="PK252_14380"
     CDS             1483..2088
                     /gene="pnuC"
                     /locus_tag="PK252_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT15941.1"
                     /translation="MLQGVWLFILNNWIELFGSISGLIYIIFSYRHSIWLWPIGIATS
                     AAYIIVFYDTALYADMLLQVYYLFVSIYGWLHWIFGKQVNKTTHKLKICRLKPYQWFI
                     SITITGTLTLFYLPFGQYFNASFPLVDGFITAGSIVATWMLTRKIVEQWIFWVILDAT
                     SVVLMIAKDKYPTAILMTIYTILAVAGYFKWLKDYKLQDND"
     gene            2081..2593
                     /locus_tag="PK252_14385"
     CDS             2081..2593
                     /locus_tag="PK252_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15942.1"
                     /translation="MIKKISLIGPECTAKTTLASELARHFGTMWIPEYAREYIENLDH
                     KYTFQDVEHIAQKQLSQINSTYPQANNWIFFDTDLIITKVWFEVVFNNCPEWINEEIK
                     KKHFALHLLCKPDIPWVADKVRENGGKNREILYFRYLDILQNYKLPYGIVEGEEKMRF
                     SNAIVLLNNL"
     gene            2612..3826
                     /locus_tag="PK252_14390"
     CDS             2612..3826
                     /locus_tag="PK252_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOT15943.1"
                     /translation="MSSPQILLSALPEKESFQMTCKTFFGLENVLANELQAIGAKEIK
                     TARRSVYFSGDKSVMYKANYCLRTALNVLVPFCTFYAKDENELYNEVYNIAWENYFTL
                     KHTFAIETTINSDFFKHSQYAALKTKDAIVDYFRAKTGKRPFIDAENPQILIHLHISD
                     KTCTLSLNSSGEPLFKRGYKVSNGAAPINEILAAGMLKLSGWTPKHNLFDPMCGSGTI
                     LIEAAMIAENIAPGVFRKQYSFENWSNFESKILEGIAEQEHDNNPKDLPVITGIDISG
                     QAIHATNKNLQNSFLNNYIKVENKDFFECKKPFEKGFIITNPPYGEKFKNNSLSRFYA
                     QIGDKLKNSFTNFETWIISPDKEALKFIGLHPEKKVKLFNGPLECSFNKYTVYEGSKK
                     QYKNNLQRPYQY"
     gene            3968..4516
                     /locus_tag="PK252_14395"
     CDS             3968..4516
                     /locus_tag="PK252_14395"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOT15944.1"
                     /translation="MQFNFDDYYKGLNKGNVLMAYHGNITAEWINGVLESIESKLDSA
                     NEDPKIKKKLYNVLVEGLQNLFHHVDDLPDEIANLFTKKFGVVVITKDCNKYIVSLGN
                     FVEHSKAQYLKDKIDKINSLTEEELKDMYKFILNHQKLSAKGGGGLGLLDMARKTGNK
                     LEYTFYQFNSNYMFFNLNVTIN"
     gene            4543..4923
                     /locus_tag="PK252_14400"
     CDS             4543..4923
                     /locus_tag="PK252_14400"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOT15945.1"
                     /translation="MEALIIEGTAKTPSVRFEAGQGTIEIKGRSIPENSIEFYKPLVD
                     WLEEYGKSPAALTKVNIQLEYFNTSSSKCILDVFKKLEAIYKAKNEVIINWYYEEDDE
                     DMLEAGEDYESIIRVPFKMIEIVD"
BASE COUNT         1793 a          896 c          848 g         1406 t
ORIGIN      
        1 attgtatgta gataccgtca accagacagc tcttacagaa accgcaatca gggcaatggt
       61 gaaagaatta gaccctcact cggcatatct gaacattgaa gaagtaaaag agctgaatga
      121 accattgcaa ggcaactttg aaggtatagg ggtaagtttt aatatcctta acgatactat
      181 ttttattgtc aacactattg ccggaggccc atcagaaaaa gttggggtca tggggggcga
      241 ccgtattatc aaaattgacg gcgaactggt agcagggatt aaaattacta ataaaaccgt
      301 ttttaaaaaa ctaagaggca acaaaggcac caaagtcgtt atttcagtat tgcgcaaagg
      361 ctctaaaaac cttatagatt tcactatcac ccgtgataaa atccccattt acagtattga
      421 cgcagcatac atgctcgata aggaaaccgg ctatatcaaa ctcgaccgtt tttcgcaaac
      481 taccaacgaa gaatttgcta aagccctaaa aaaactacag tcacaaaaga tgaaaaacct
      541 aatccttgac cttacaggta acggtggagg tttattagat gaagcagcta tgcttgctga
      601 tcaatttctc gaaaaagaca aacttttggt ttataccaaa ggccgcaaaa cccgcagaca
      661 agatttcttc tcaaccagcc aggggctatt tgaaaaaggc aaactggtaa ttatgattga
      721 cgaaaactcg gcttctgcca gtgaaattct ttcaggggca atacaagact gggacagggg
      781 catcattgtt ggccgtcgct cttttggcaa aggcctggta caaagacctt tgatgtaccc
      841 tgacggttca atgctaaagc ttacaatagc tcattattac accccttcag gaagagatat
      901 tcaacgacca tataaaaatg gtaactctga cgaatattat aacgacttta ttaacagata
      961 taaacacaat gaactttcaa ataaagacag catccacttt gtagattcat tgaaaaaact
     1021 gaccttagta ctaaaacgcc cggtatttgg aggtggtggc attatgcctg atatatttgt
     1081 cccaattgat acttcaatgt attcagatta ttatcgggat ttgctcagaa acggtactct
     1141 ttataagttt gtcctcaatt acatcgatac aaaccgtata gcacttaaaa aacaatatcc
     1201 cgactttcaa aaattcaatg catcttattg tgcagaacaa ctgttccctg ctatgattga
     1261 ttttgcaagc aaagacaaca tcaaatttaa tcaacaacaa tttgaacaat caaagaatgc
     1321 tatttcaaaa ctactaaaag catacatagc ccgcgatatc tgggattctt cagagttttt
     1381 tcaggtattt aactccgatg accccattat caaagaagcc ttaaaagcca ttgaccaata
     1441 cgatactata atttctccac ctaaaaagtg aaacagattt aaatgttgca aggagtatgg
     1501 ctattcatcc taaataactg gattgaatta tttgggagca tttcaggctt aatttacatt
     1561 atattttcat acaggcacag catttggctt tggccaattg gtattgccac atctgcagct
     1621 tacattatcg ttttttacga tacggcattg tacgccgata tgttattaca agtgtattat
     1681 ttatttgtaa gcatctatgg ttggcttcac tggatttttg ggaaacaagt aaataaaaca
     1741 acacacaaac taaaaatatg cagacttaaa ccatatcaat ggtttataag cataaccatt
     1801 acaggaacct tgacattgtt ttacttacca tttggtcaat attttaatgc ctcatttcca
     1861 ttggttgatg gttttattac agccggaagc atagtagcca cctggatgtt gacccgaaag
     1921 attgttgaac aatggatatt ttgggtcatc cttgacgcaa cttctgtagt tttgatgata
     1981 gcaaaagata aatacccaac agctatttta atgacaatat atacaatact tgcagtagca
     2041 ggatatttca aatggcttaa agattataag cttcaggaca atgattaaaa aaatatctct
     2101 aatagggcct gaatgtacag caaaaacgac tcttgcttca gaactggcaa ggcattttgg
     2161 tactatgtgg atcccggaat atgcccgcga atacattgaa aacctggatc ataaatacac
     2221 ttttcaagat gttgaacata tcgcccaaaa acaacttagc caaataaatt caacatatcc
     2281 acaagccaac aactggattt ttttcgatac cgaccttatt atcacaaaag tttggtttga
     2341 agttgttttc aacaattgtc ccgaatggat aaacgaagaa atcaaaaaaa aacattttgc
     2401 attgcacctt ttatgcaaac cagacatacc ctgggttgca gataaagtcc gtgaaaacgg
     2461 aggaaaaaac cgcgaaatac tttatttcag atatttagat atcttacaaa attataaact
     2521 cccctacggc attgttgaag gtgaagaaaa aatgagattt tccaatgcca tcgtgctttt
     2581 aaacaattta tagatttcta atatatacaa aatgtcatca ccacaaattt tactttctgc
     2641 actgccagaa aaagaatcat ttcaaatgac ctgcaaaaca ttctttgggc ttgaaaatgt
     2701 tttggccaat gaacttcagg ctatcggggc aaaggagata aagactgctc gccgttctgt
     2761 ttatttctca ggcgacaaaa gcgttatgta caaagcaaac tattgtctgc gtacagcctt
     2821 aaatgtttta gtgccttttt gtacattcta cgctaaagac gaaaatgaac tgtacaatga
     2881 agtatataat atcgcctggg aaaattattt cacattgaaa catacatttg ccattgaaac
     2941 aacaattaat tcagactttt ttaaacattc acaatatgct gcactgaaaa ccaaagatgc
     3001 cattgtagat tatttcaggg ccaaaacagg caaaaggcct ttcattgatg ctgagaatcc
     3061 ccaaatactt atccacctgc atatatcgga caaaacctgt acattgtcac tcaatagttc
     3121 tggagagcct ctttttaaga gaggatacaa agttagtaat ggagccgccc caataaatga
     3181 gatacttgca gccggtatgc tcaaactttc gggctggact ccaaagcata atttgtttga
     3241 ccctatgtgt ggctcaggta caatattgat tgaagctgcc atgatagctg aaaacattgc
     3301 tcccggagta ttcagaaaac aatacagctt tgaaaactgg tcaaactttg aaagtaaaat
     3361 attagaaggt atagcagaac aggaacatga caacaacccc aaagacttgc ctgtcattac
     3421 aggtatagac atctcagggc aggcaataca tgccaccaac aaaaatcttc aaaacagttt
     3481 tctaaacaat tatataaaag ttgaaaataa agatttcttt gagtgcaaaa aaccctttga
     3541 aaaaggcttc atcattacca atccacctta cggcgaaaag tttaaaaata actcattaag
     3601 ccggttttat gctcaaattg gcgataaatt aaaaaacagc ttcaccaatt tcgaaacctg
     3661 gattatatca cctgataaag aagctttgaa atttatcggg ttacaccccg aaaaaaaagt
     3721 aaaattattt aacgggccgc tcgaatgtag ttttaataaa tacaccgttt atgaaggttc
     3781 aaaaaaacaa tataaaaata acctacaaag gccttatcag tattaatgac aaaaatgaaa
     3841 tattaaaaaa atgacatttt taaaataata aaaaaaaaaa tgctattttt aaaactcttt
     3901 ttgtgataaa ataaaatgct atatttgttg ataaatatta tcctcaagct acaaaccgtt
     3961 aaagtgtatg cagttcaatt ttgatgacta ttataagggt ttaaacaaag gcaatgtatt
     4021 gatggcctat catggcaaca ttaccgctga atggattaac ggggtacttg aaagtattga
     4081 aagtaaactg gatagtgcaa acgaagaccc taaaatcaaa aaaaaactgt acaatgtttt
     4141 ggttgaagga ttacaaaact tatttcacca tgtagatgat ttgccagacg aaatagccaa
     4201 tttgtttacc aaaaaatttg gggttgtcgt aataacaaaa gattgcaata aatacattgt
     4261 ctcgttaggt aactttgtcg aacatagtaa agcacaatat ttaaaagaca aaattgacaa
     4321 aataaactca ttgacagaag aagagctaaa agatatgtac aagtttatcc ttaaccacca
     4381 aaagttatct gccaaaggag gcggaggttt aggattgtta gatatggccc gtaaaactgg
     4441 caacaaactt gagtacactt tttatcaatt taatagcaat tatatgtttt tcaatttaaa
     4501 tgttactata aattaaaaag ataattttaa aagcccttat atatggaagc attaatcatt
     4561 gaaggaacgg caaaaactcc aagcgtaaga tttgaagcag gacaaggaac aattgagatt
     4621 aaaggaagat ctattcccga aaactcaatt gaattttaca aaccattggt tgactggctt
     4681 gaagaatatg gaaaatcccc ggcagccctc accaaagtca atattcagct agagtatttc
     4741 aataccagtt catcaaaatg tatccttgac gttttcaaaa aactggaggc catttacaag
     4801 gccaaaaatg aagttattat taactggtac tatgaagaag atgatgaaga catgttagaa
     4861 gcaggcgaag attacgaatc gattattcgt gtgccattta aaatgataga aatagtagat
     4921 taatctgctt ttctctaaaa aaa
//
LOCUS       DAOSPF010000125         4809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_17734_length_4809_cov_7.449727, whole genome shotgun sequence.
ACCESSION   DAOSPF010000125 DAOSPF010000000
VERSION     DAOSPF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17734_length_4809_cov_7.449727"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1341
                     /locus_tag="PK252_14405"
     CDS             121..1341
                     /locus_tag="PK252_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT15946.1"
                     /translation="MSKRNSYIKRTLYLDKIKPFIDKDIIKVFKGQRRVGKSYLLLQT
                     MDYIKKIHPQANIIYINKELDEFWELKDAQTLLDYVKEKKAEKGKTYLFVDEIQDIRE
                     FEKALRSLLAEGLFDIYCTGSNAKLLSGELATLISGRHVEIEVFGLSYLEFLEFYKLA
                     DSDKALENYLHYGGLPFLIHLPMEDAVVYEYLKNIYQTILLKDIVSRFNIRNVAFLER
                     LVRFLGDNVGSLVSSKKISDFLKSQQVNISPQVVLDYLSHIVSACFIYKVSRTDIEGK
                     KIFEVGEKYYFEDIGVRNAIVGYKTTDIGKIMENVCYKHLRTYGYNVLVGISGEKEID
                     FVCEKQGEKVYLQVCYLLSDEKTIEREYENLLALKDNYPKIVVSMDAVKGQNTYQGIH
                     SFHLREFLTTTHFG"
     gene            complement(1423..2670)
                     /locus_tag="PK252_14410"
     CDS             complement(1423..2670)
                     /locus_tag="PK252_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOT15947.1"
                     /translation="MAIIKPFRAIRPKKELAKDVASRPYDVLNSEEAKLEAQGNPYSF
                     LHVTKSEIDLPGVTDIHTAVVYNKAKSNLQEMIAKGWLFQESKACYYIYAQKMWGKTQ
                     YGIVGCAAVEDYLNNIIKKHELTRPDKEEDRKEHIRVTNVNAEPVFFAYPDNKELDGI
                     VARYTANAAEYDFTLSDGVGHQLWVINNDADIARIEEIFRKDIPFTYVADGHHRTAAA
                     AHVGEERKEKNANHTGKEEYNYFMAVHFPASQLTIIDYNRVIQDLNGLSETEFIEKLK
                     KSFTVEDKGTTEYKPAKLHNFSMYLGGRWYSLTPNAGLYDDNHPIECLDVTILSNLVL
                     TPILDIVDLRKSTRIDFVGGIRGLGELKKRVDSGEMVAAFALYPVSMKQLIDIADTGN
                     IMPPKTTWFEPKLRSGLFVHDLE"
     gene            2776..3516
                     /gene="truA"
                     /locus_tag="PK252_14415"
     CDS             2776..3516
                     /gene="truA"
                     /locus_tag="PK252_14415"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT15948.1"
                     /translation="MQRYFIQLSYNGFAFVGWQKQPCGVTVQGELEKALTTLLRTNVE
                     VVGAGRTDTGVHASYYIAHFDLPLAIDHPDNVVFHLNRILPYEIAVQKIFPVPANKHA
                     RFDAVLRTYKYVITTEKNPFAKTLQWHTHFLLDIEKMNQAASLLTKHEDYTSFCKLHG
                     NNITNICHVKEAFWTKINNEIIFTISADRFLRNMVRAIVGTLVDVGRGKIEVADFEKI
                     ILTKNRNAASASAPAHGLYLVNIQYDFF"
     gene            3633..4796
                     /locus_tag="PK252_14420"
     CDS             3633..4796
                     /locus_tag="PK252_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15949.1"
                     /translation="MLLPKALHYYKDKDFDIQLKARFLYYLILIVMYSMTGIYIYSLT
                     VQINSPLYKGIYPPLLIVETTILLVFYLCLALLKKGHLPLSSHILLVFTFVCLWSLIW
                     LDIGEGVTRFDTVVYILAFLSTLPIIVIHNKNMILAYTGLNLLGVFAFALYAKYYFAI
                     AWNVFADYLFDTSVSLVFIGIVAYNNFRINKISSEKIIGELKERKIAENALLQSEGRY
                     KDMTELLPQTVFEADISGKITYTNKAGMEQFGYSEEDFNTGVNIINTVAPEDRERAAE
                     NYRQTINLVQTSKGKYYTALRKDKTRFPILLYTSVIHEQDKPVGVRGIIIDISERKKI
                     EEELKQSEDTFKTLLEMLPYAVAVSDMNGRFIMVNNAFCEELMYHTPEGGGLF"
BASE COUNT         1475 a          851 c          939 g         1544 t
ORIGIN      
        1 acttacagtc cgttagaaca acccaccgcc ttcaggcgtg tggtacatga gtttgcaaat
       61 atttcaatta taattgaaat attaattatt tttaagcact atttaattta tgaatgaaat
      121 atgtcgaaaa gaaatagtta tatcaaaagg actttatatc ttgacaagat aaaacctttt
      181 atagacaagg atattataaa agtatttaaa ggtcagcgtc gggtaggcaa gagttacctg
      241 ttgctgcaaa caatggatta cattaagaag attcatcctc aggccaatat tatttacatc
      301 aataaagaac ttgacgaatt ttgggaattg aaagatgccc agactttgct ggattatgtt
      361 aaagaaaaga aggcagaaaa gggtaaaacg tatttgtttg tggatgaaat acaagatatc
      421 cgggaatttg aaaaagcact gagaagcctg ttagctgagg ggttgtttga tatttattgt
      481 acagggagca atgcaaagct tttgtctggc gaattggcaa cattgatcag tggacgacat
      541 gttgaaattg aggtgtttgg gctttcttat ctcgaatttc ttgagtttta taaactagcc
      601 gattctgata aggcattgga aaactacttg cattatggag gcttaccttt tttaatccac
      661 ttgccaatgg aagatgctgt tgtttatgaa tatttgaaga atatatatca gacaattttg
      721 ttaaaagaca ttgtgagcag gtttaatatt agaaatgtag cctttcttga acgtttagtc
      781 aggtttttag gagataacgt tggtagtttg gtttcatcaa aaaaaattag cgattttctg
      841 aaatcacaac aagtgaatat ttctccgcag gttgttttag attacctgag ccacattgtt
      901 tcggcttgtt ttatatataa agtcagcaga acagatattg aagggaaaaa aatattcgag
      961 gtgggtgaaa aatattattt tgaggacatt ggggtgcgta atgctattgt tggttataaa
     1021 accactgata taggcaaaat aatggagaac gtatgttata aacatttgcg gacatatgga
     1081 tataacgttc ttgttggcat ttctggtgaa aaagagattg attttgtatg cgaaaaacag
     1141 ggagaaaaag tttatttaca ggtttgttat ttgctgtcag acgaaaaaac tatagagcgt
     1201 gaatatgaaa acttgctggc tttaaaagat aattatccaa aaattgtagt gtctatggac
     1261 gctgtaaaag ggcaaaatac ttaccaaggc attcattctt tccatttaag ggaatttttg
     1321 actacaaccc actttggata gcaggttaca agtttttttt aaagaaaaaa cccgaaagga
     1381 ttaaatcttc cgggcttttt ctttaaaatt ataatcttcg tcttattcta agtcgtgaac
     1441 aaaaagtccg cttcggagct ttggctcaaa ccatgtggtt ttggggggca tgatgttgcc
     1501 agtgtcggca atgtcaataa gctgtttcat cgaaactgga tacaatgcaa atgcagcaac
     1561 catttcgcca ctgtccacac gttttttgag ttcgcctaat ccacggatac cacctacgaa
     1621 atcaatacgg gttgatttgc gtaagtcaac aatgtcgaga ataggagtga gtacaaggtt
     1681 tgaaagtatc gttacatcta aacactcgat aggatgattg tcatcataca agcctgcatt
     1741 aggggtaagc gaataccaac gtccgccaag gtacatgcta aagttgtgaa gctttgcagg
     1801 cttatattct gtagtgcctt tatcttcaac ggtaaacgat tttttaagtt tttcaataaa
     1861 ctcagtctct gacaatccgt taaggtcttg aataacgcgg ttgtagtcga tgatagtcag
     1921 ctggctggca ggaaaatgaa cggccatgaa gtagttgtac tcttctttcc cggtatggtt
     1981 ggcgtttttt tctttacgtt cttcgccaac gtgcgcagct gcagcagttc tgtggtgtcc
     2041 atcagctacg taggtaaatg gaatatcttt tctgaaaatt tcttcaatac gggcaatgtc
     2101 cgcatcgttg tttattaccc aaagttgatg gcctacacca tctgacaacg taaaatcata
     2161 ttcggcagca ttagccgtgt acctggctac aataccatca agctctttat tgtctggata
     2221 tgcaaaaaat acaggctcgg catttacatt ggtaaccctg atgtgttctt tgcgatcttc
     2281 ttctttgtct ggtcttgtca attcgtgctt tttgataatg ttattgagat aatcttcaac
     2341 agcagcacaa cccacaatac catattgtgt ctttccccac atcttctggg cataaatgta
     2401 ataacaagct ttgctttcct gaaaaagcca acctttagct atcatctcct ggagattgct
     2461 tttggctttg ttatagacaa ccgcggtatg aatgtctgta acaccaggta agtctatctc
     2521 agattttgtt acatgaagaa aagaataagg attcccctgt gcttctaatt ttgcctcttc
     2581 tgaattgagc acgtcataag gccttgatgc tacatcttta gccaattctt ttttaggcct
     2641 gatagccctg aatggtttta ttattgccat agttttgaaa ataagggtgc aaacgtacaa
     2701 gttttttaaa aattaagcac gattctgctt aaatttgtga ccaacaatta aggtttttac
     2761 gtaattctcg tagctttgca acgttatttt atacaactga gctataatgg ttttgctttt
     2821 gtagggtggc aaaagcaacc ctgcggagta accgtccagg gagaacttga aaaggcttta
     2881 acaactttat tacgcaccaa tgttgaggtt gttggagcag gccgcacaga taccggagtc
     2941 catgcttcat actacattgc acactttgat ttacctttag caatagacca tcctgataac
     3001 gttgtgtttc atctcaaccg tattttacct tacgaaattg ccgtgcaaaa aatttttcct
     3061 gtgcctgcca acaaacatgc ccgttttgat gctgtattac gtacttataa atatgtaatt
     3121 accactgaaa aaaatccttt tgccaaaacg cttcaatggc atactcattt tttgctcgat
     3181 attgaaaaga tgaaccaagc tgcttcttta ttgacaaagc atgaagatta taccagtttc
     3241 tgcaagcttc atggaaacaa cattaccaat atttgccatg taaaagaggc tttttggacg
     3301 aagattaaca atgagataat ctttaccatc agcgccgaca gatttttgcg caacatggta
     3361 cgtgctattg tcggaacatt ggtagatgta ggaagaggaa aaatagaagt tgccgatttt
     3421 gaaaaaatca ttcttacaaa aaaccgaaat gcagcatctg cttctgctcc tgcacacggc
     3481 ttataccttg ttaatattca atacgatttt ttttaatggc ctaaggagtt tttgacactt
     3541 tttctgtttc gttcaggtat attttctaac tttatctaat gataaagaaa cggaaatgtg
     3601 ttaatttgca tgtacgtata atccaataac caatgctcct gcctaaagcc ctgcattact
     3661 ataaagataa agactttgac atacagttga aagctcgttt cctgtactac ttaatcctta
     3721 ttgtaatgta cagtatgaca ggtatttaca tttactcctt aacagtacaa atcaacagcc
     3781 ctctgtataa aggcatttat cctcctttat tgatagttga aaccaccatt ttattggttt
     3841 tttatttatg tcttgcgtta ctcaaaaaag ggcatctgcc tctttcctca catatactat
     3901 tagtcttcac attcgtgtgt ttgtggtctt taatctggct tgacattgga gaaggcgtaa
     3961 cacgttttga cacagtagtt tacatattag cctttttgtc caccctgcct attatcgtaa
     4021 tacacaacaa aaatatgatt ttggcctata cagggttaaa tttattaggg gtatttgctt
     4081 ttgcactata tgcaaaatac tattttgcca ttgcctggaa tgtttttgcc gattatttgt
     4141 ttgacacctc tgtatcactt gtatttattg gcattgttgc gtacaataat tttaggatta
     4201 ataaaatttc ttccgaaaaa atcattggag agctaaaaga acgaaagatt gctgaaaatg
     4261 cccttttaca aagcgaaggc aggtacaaag acatgacaga attgctgccc cagacagttt
     4321 ttgaagcaga tatatcagga aaaataactt ataccaataa agccgggatg gagcaatttg
     4381 gctattctga ggaggatttt aatacaggcg taaatattat taacactgtg gctcccgaag
     4441 atagggaaag ggctgctgag aactaccgtc agacaatcaa tttagtgcaa acaagcaaag
     4501 gaaaatatta taccgccctg agaaaagaca aaacccgatt ccctatactt ttatacacca
     4561 gcgttattca tgagcaggat aagcctgtag gcgtaagagg tataattatc gacatctccg
     4621 aacgtaaaaa aatagaagaa gaactcaagc aaagtgaaga caccttcaaa acattgctcg
     4681 aaatgttacc ttatgctgtt gctgtttctg acatgaatgg ccgttttatc atggtcaata
     4741 acgccttttg tgaagaactc atgtaccaca cgcctgaagg cggtgggttg ttctaacgga
     4801 ctgtaagtc
//
LOCUS       DAOSPF010000126         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_18212_length_4709_cov_7.183283, whole genome shotgun sequence.
ACCESSION   DAOSPF010000126 DAOSPF010000000
VERSION     DAOSPF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18212_length_4709_cov_7.183283"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..928
                     /locus_tag="PK252_14425"
     CDS             176..928
                     /locus_tag="PK252_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15950.1"
                     /translation="MQSFKNKAQYKELIQKTVTSFGFSADMYQSAFARVFEELFENNA
                     RCACLREFYKTNVPEIIVDELIKVKKSNKKNFLTFFEDCQTLNNELNELTHKIRRSCS
                     CNDKTCNNAEHNKIDITKFPLFNALVEDKNLKIYKKELIASPEFISFFENNVGKYGLF
                     FLYNKDKELLFIGKSENLGAELLNIIYERNIEGYVAVAYTKTISDIYVYENYCIIREL
                     PLLNTKNMAVDGLSFNLEELKKSELVKIYGNN"
     gene            complement(939..1250)
                     /locus_tag="PK252_14430"
     CDS             complement(939..1250)
                     /locus_tag="PK252_14430"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT15951.1"
                     /translation="MYRPEIGISDHTLSLILKVVQENPKVSEVILFGSRAKGNFVSGS
                     DIDLALKGNEINLNDILELSVNLDKLNLPYKFDLIIYDRIQESALIEHITRNGICLFR
                     K"
     gene            complement(1257..1679)
                     /locus_tag="PK252_14435"
     CDS             complement(1257..1679)
                     /locus_tag="PK252_14435"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOT15952.1"
                     /translation="MDNKKDIRWQQRFINFNKAFKQLERFMEHDNLNEMEEQGLIKAF
                     EYTYELSWKTLQDLLKEKRYIDIVGPKPVIEQSFQDGYITDGHGWMKMHVSRNLSSHT
                     YDQQTANEIISGIKNEYFILLKNLVLRLDEELKKENRA"
     gene            complement(1781..3016)
                     /locus_tag="PK252_14440"
     CDS             complement(1781..3016)
                     /locus_tag="PK252_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT15953.1"
                     /translation="MKHLGIYLSNSVNKKQLIDEIFSNSLLKEHLDISTLKGALHSPI
                     TIDKIIDEEVRHDKFPIKTRENASLMSMSSGQQRKALLAYLIDQKPDFIILDDIFSSI
                     DSDTQQYILRYLEQHSKNIQYIQLFYRKHDVLSFIDTVISVNEQFQVISQESLELFKN
                     KHESSEHLNNVTLPYLFNEAHLDIDPLIQLNSVSAAYGDKPVLNNINWTIRPGEFWQL
                     KGPIGSGKSTLLSMIIGDNPRGYGQDMMLFGCKKGSGESVWDIKKQIGYFYPTLTQLF
                     THDDTVENMIISGILDSIGLYKKPTDLHKRIANAWLQVLGSSFQNKRFQELSSGQQRI
                     VLVVRAMVKQPPLLILDEPTAGLDDHNAQLFINLVSAIASQRKVAIIYVSHRSEPGLL
                     PDKVLELVPTHHGSVAKVL"
     gene            complement(3115..4128)
                     /gene="murB"
                     /locus_tag="PK252_14445"
     CDS             complement(3115..4128)
                     /gene="murB"
                     /locus_tag="PK252_14445"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT15954.1"
                     /translation="MLQVERAISLKPYNTFAIDIVADYFVRIKSENELIEILNNPQYK
                     ALPRLILGTGSNVLLTAAFHGLVLKMEISGIEVIAENEQHVIVRVGAGQDWDNFVEYC
                     VKQNWCGIENLSLIPGTVGACPVQNIGAYGVEVKDCIEKVEGIDCTTLKIFSLSNEDC
                     QFGYRSSIFKKSMAGQYVITHVIFKLNKQLTPILSYGNIKDGIEKTGVVSLENIRKVI
                     IDIRNSKLPDPKDVPNAGSFFKNPVIANIKAEKLKKKWPNIVLYPIDCETTKVSAAWL
                     IDNAGLKGFTYKDAKIHDRQPLVLTNIGNASGQDILDLATIVLQKVKEKFDIELEMEV
                     NKV"
     gene            complement(4225..4359)
                     /locus_tag="PK252_14450"
     CDS             complement(4225..4359)
                     /locus_tag="PK252_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15955.1"
                     /translation="MSERYRGFFVIYPAMQENYYVYIIESLTDSTLYKGYTTNYKRRQ
                     "
BASE COUNT         1420 a          824 c          854 g         1611 t
ORIGIN      
        1 agttaaagat ttgtttaaaa aaattactga ggttttaaat ctgttacaaa tttagaaata
       61 tgtctcaata gcacaaaaat caatgttttt tttggatgat gaaatttgtt aatatttatc
      121 gcttaaatat ctttataaat cattaattta gttaagcttt tccaaaatga atattatgca
      181 atcgtttaaa aataaggcac aatacaaaga gttaattcaa aaaactgtta caagctttgg
      241 tttcagtgcc gatatgtatc aaagtgcttt tgcccgggtc tttgaggagt tgtttgagaa
      301 taacgccaga tgtgcttgtc tcagggagtt ttataaaacc aatgtgccgg agattattgt
      361 agatgagctg attaaagtga aaaaatccaa taaaaagaac tttcttactt tttttgagga
      421 ctgccagacg ttaaataatg agttaaatga acttacgcat aaaataaggc gttcatgcag
      481 ctgcaatgat aagacatgca acaatgctga acataacaag atagatatta ctaaattccc
      541 tttgtttaat gcattggttg aggacaaaaa cctgaagata tacaaaaaag agcttattgc
      601 ttcgcctgag tttattagtt tttttgaaaa caatgtcggc aagtatggcc tgttttttct
      661 ttataacaag gataaggaat tgctctttat cggcaaaagt gaaaaccttg gtgcagagct
      721 actcaacatc atttacgagc gcaatattga aggatatgtt gcagtagcct ataccaaaac
      781 catctcggat atatacgtat atgagaatta ctgcatcatt cgcgaactgc ctttgctcaa
      841 taccaagaat atggctgtgg atgggctgag cttcaatctg gaagaactaa agaaaagtga
      901 gttggtgaag atatacggga ataattagta aatgggactt attttctgaa caagcaaata
      961 ccatttcttg taatatgctc aattaaagca gattcttgta tcctgtcata aattattaaa
     1021 tcgaatttat agggtaaatt cagtttgtcc agattaacag acaattccag aatatcatta
     1081 aggttaattt cattgccttt tagcgccagg tcaatgtcag aaccactgac aaaattacct
     1141 tttgcccgtg aaccaaataa aattacctca ctaacttttg gattttcttg cacaacttta
     1201 agtattaaac ttaaagtatg gtcgctaatt ccaatttctg gtctatacat attcacttaa
     1261 gctctgtttt ctttttttaa ttcttcgtcg agacgtaaaa ctaaattttt taaaagaata
     1321 aaatattcat tctttattcc gctaataatt tcattagcag tttgttgatc ataagtgtgg
     1381 ctggataaat ttcggctaac atgcattttc atccatccat gcccatcggt tatatagcca
     1441 tcctgaaaac tttgttctat aacaggtttt ggtccaacaa tatcaatata tcttttttct
     1501 ttcaacaaat cctgaagcgt tttccagctt agttcgtatg tatattcaaa agcttttatc
     1561 aggccttgct cttccatttc gttcaggttg tcatgttcca tgaacctctc cagttgttta
     1621 aaagctttat tgaagttgat aaatctttgc tgccaacgta tatctttttt gttatccatt
     1681 ttcaactcat tgttttaaag caaagataat tttttttgaa tcaggaatgt atatctgcct
     1741 aacaataaga gttttattag agaggtgata taatatcttt ctacaacacc tttgccaccg
     1801 acccatgatg cgttggaact aattccaaca ccttatcagg taataatcct ggctcacttc
     1861 ggtgcgaaac atagatgatg gctacctttc gttgtgaggc tatagctgat accagattta
     1921 taaagagctg tgcattgtga tcatcaaggc cggctgtagg ctcgtcaagt atgagcaatg
     1981 gtggctgttt taccatagcc cgtacaacaa gtacaatccg ctgttggcct gaagatagct
     2041 cttgaaacct tttattctga aatgaagatc ccaacacttg taaccatgca ttggcaatac
     2101 gcttgtgcag gtcagtgggc tttttgtaaa gtcctataga atctaaaatt ccggaaatga
     2161 tcatgttttc aaccgtatca tcgtgagtaa agagctgggt aagggtaggg tagaagtaac
     2221 ctatttgttt ttttatatcc cacacggact ccccgcttcc ttttttacac ccgaacagca
     2281 tcatatcttg cccgtaacct ctggggttat ccccaataat cattgacagt agcgtgcttt
     2341 tgccagaccc aattgggcct ttgagttgcc aaaattcgcc cggacgtata gtccagttga
     2401 tattgttcag cactggctta tctccgtatg ctgccgacac agaattgagc tgtataagcg
     2461 ggtctatgtc aaggtgagct tcattaaaca ggtatggtaa ggtgacattg tttaaatgtt
     2521 ctgaagattc atgtttattc ttgaacagct caagtgattc ctgcgaaata acctgaaatt
     2581 gttcatttac agaaataact gtgtctataa agctcaatac atcgtgtttt cgatagaata
     2641 gctgtatgta ttgtatattt ttagaatgct gttcgaggta gcgtagaata tattgctgtg
     2701 tatctgagtc aatgctgctg aatatatcgt cgaggataat aaaatcaggc ttttggtcga
     2761 tgagataggc cagaagtgcc tttcgctgtt gtccactcga catactcatc aggctggcat
     2821 tttcgcgagt tttgatagga aacttatcgt ggcggacttc ctcgtcaata attttatcaa
     2881 tggtgatggg cgaatgcaat gcccctttca atgttgaaat atctaaatgc tcctttagca
     2941 gggagttaga aaagatttca tcaatgagct gtttcttgtt aaccgagtta gagagatata
     3001 tgccaaggtg tttcattttg ccagccattt accgtgtcac ttgtaaatgt tttttttgag
     3061 ttgttattta aaaatacata attcaattct tttagtaatt tttacattag ctaattatac
     3121 cttgtttact tccatttcaa gctctatatc aaatttttct tttacttttt ggagtacaat
     3181 agttgcaagg tcgaggatat cctgaccaga agcattgcca atattagtta aaactaatgg
     3241 ctgtctatca tgtatcttgg catctttata tgtgaaccct ttcaatcctg cattatcaat
     3301 gagccaggca gcagatactt ttgttgtttc acaatcaatg gggtaaagaa caatattagg
     3361 ccattttttt ttcaattttt cagcttttat attggctatt accgggtttt tgaaaaaact
     3421 gcctgcgttt ggtacatctt taggatcagg aagcttgcta tttctgatat ctataatgac
     3481 ttttcgaata ttttctaacg aaactacgcc tgttttttca ataccatctt taatatttcc
     3541 atagcttagg atgggagtta gttgcttatt taatttaaat atgacatgag tgataacata
     3601 ttgtccggcc attgattttt tgaagatgct tgagcgatag ccaaattgac aatcttcatt
     3661 gcttaaactg aatattttta aagtggtaca atctattcct tctacttttt cgatacagtc
     3721 ttttacttca actccataag cccctatgtt ttgtaccgga catgccccta cagtgccagg
     3781 aataagtgaa aggttttcta ttccgcacca attttgtttt acgcaatatt caacaaaatt
     3841 atcccaatct tggcctgctc caacccggac aattacatgt tgctcatttt ctgcaataac
     3901 ctcaataccg gaaatttcca tttttagcac aaggccatga aaagctgctg taagcagtac
     3961 attgctccct gttccaagga tgagcctggg taaagcttta tattgtggat tgtttaatat
     4021 ttcaattaat tcgttttcag atttgatgcg tacaaaatag tcggcaacaa tgtcaattgc
     4081 aaatgtatta tatggtttaa gcgatattgc tctttctact tgcagcattt tattaatttt
     4141 tgtcaaagat aagattaata tgaattaacc aattaatgaa ttaaccaatt agctgatatt
     4201 ttattctatt gttgttgtca attattattg tctccgtttg taattggtag tataaccttt
     4261 gtaaagcgta ctatctgtaa ggctttcaat aatatataca taataatttt cttgcatggc
     4321 agggtaaata acaaaaaagc ctcgatatct ctcgctcatc gcagtttgtc aataaaagcg
     4381 gttcgacgta gcgggacgct acaccgaatg gggggctaag aatacagtcc aagtcgaatg
     4441 tttgaactat ttagagaagg ttacaggccg gatagcaaag gtgcaaactt caataaaaag
     4501 aggcacaagg ttccaaaact ctcgccataa gagggttatt gactacttgg gcgaactttt
     4561 aaagagggca aaaatgggca ttaaattgta cagttgtaaa tatcataaaa tcagcgtttc
     4621 gaaaaatgtt agtcagaagc cgaatacgtg tttaaattta atttataatt ctgggatggg
     4681 tgaaatccct cgcctttagg cgaagcatt
//
LOCUS       DAOSPF010000127         4639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_18577_length_4639_cov_7.743019, whole genome shotgun sequence.
ACCESSION   DAOSPF010000127 DAOSPF010000000
VERSION     DAOSPF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18577_length_4639_cov_7.743019"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PK252_14455"
     CDS             <1..1393
                     /locus_tag="PK252_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOT15956.1"
                     /translation="SYPAIIKAFNPYYAIHFLSNYPNAYVLLGAIFLCTTGAEALYSD
                     LGHCGAANIRISWVYVKSTLIINYLGQGAWVLNHQGFSGNPFFAIMPSWFLLIGVAMA
                     TLAAIIASQALISGSFTLISEAISLNFWPKLKKIYPSNVKGQVYIPAVNWFLLAGCIL
                     VILIFQKSSKMEAAYGLSITITMLMTTTLLLFYLYSKHIHFGLIVLVGLVYVFIESNF
                     LIANLYKFIHGGWFTILLATLFFLVMYGWYSARKIRNRYLTFDKLDDYMPVIAEMSKD
                     NKIPKYSTHLAFLTHANRKDEIEKKIIFSILNKKPKRADVYWFLHVNILDDPDTFEYD
                     VQTIVPGIAYKVNFHLGFKIEPRINMYFNQVLNEMKEHKEIDLTSKYNSLKKYNIDAD
                     FAFIIVERIFNFDMDFSLHDKIIVGFHKFLKYLGISEAYAYGLDMSNIVTEKVPINSI
                     HQVKRKIHRIKRI"
     gene            1396..2754
                     /gene="radA"
                     /locus_tag="PK252_14460"
     CDS             1396..2754
                     /gene="radA"
                     /locus_tag="PK252_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT15957.1"
                     /translation="MAKTKTRYVCQSCGAESSKWLGRCPSCNEWNSFVEEVVAKEVSR
                     LSDRPVYAASTPIPIGKVDFTERKRIDTTFREVNRTLGGGLVPGSMILLGGEPGIGKS
                     TLALQLALKMRDIKTLYISGEESAEQIKLRAIRLGDGNDNCHLLCETSLETILSTLEQ
                     FAPQMVIIDSIQTLQAEYIESSPGSVSQIRECSSRLLRYAKTSAVPVILIGHIVKDGS
                     LAGPKVLEHMVDTVLQFEGDRQYFFRILRSSKNRFGSTSEIGIFEMQQSGLVEILNPS
                     EILMSHSQQALSGLAIAAALNGMRPYMIETQALVSSAVYGTPQRSGTGFDFRRLNMLL
                     AVLEKRAGFRLGAKDVFLNISGGIKVDDPAIDLAVAAAILSSNADVAIPQKTCLCGEI
                     SLSGEVRPVYRIEQRIAEAEKLGFERIILAHSAAKNNISTKHIKTVYISKIEQLPKLL
                     FG"
     gene            complement(2761..3123)
                     /locus_tag="PK252_14465"
     CDS             complement(2761..3123)
                     /locus_tag="PK252_14465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOT15958.1"
                     /translation="MNWIVKRIKSFGYAFAGIYRLFRHEANAQIHLLATIVVISAGIY
                     FQLRALEWALVCLAIGVVIAAEAFNSAIEKLTDGMYPEKHDNAMHVKDMAAGAVLIAA
                     IAAVAIAVFVFGGHLGLF"
     gene            complement(3176..3796)
                     /locus_tag="PK252_14470"
     CDS             complement(3176..3796)
                     /locus_tag="PK252_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT15959.1"
                     /translation="MHRFISFLVFSLGIFSLSYSQNIDVKILDRLNSPNELKADPAMK
                     FISNSTTYAVVGVPLCIGATALVKRDSAMLSKAIAIGASLAVAEGFAYALKYTVKRKR
                     PFETYDYICKKLDGGDPSFPSGHTTAAFSTATSLSLAYPKWYVIAPSYVWATSVGYSR
                     IHLGVHYPSDVLAGAVLGTASAYATYKLNLWFHKKYLKNRRILKWY"
     gene            4121..4582
                     /gene="tnpA"
                     /locus_tag="PK252_14475"
     CDS             4121..4582
                     /gene="tnpA"
                     /locus_tag="PK252_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOT15960.1"
                     /translation="MANTYTQIYLQLVFSTLGRENIIPQKHKEELHKYASGIIENRNH
                     KLLAINCMPDHVHILIGYKPAQPLPDLMRDIKAVTSGFINDKKWLPGKFRWQEGYGAF
                     SYSHSQIGDVIKYINNQESHHKKISFKEEYLVFLNKYQVEYSEQFLFEFYE"
BASE COUNT         1374 a          933 c          929 g         1403 t
ORIGIN      
        1 ttcgtatccg gcaattatta aagctttcaa tccatattac gccatccatt ttttgtccaa
       61 ttatcctaat gcatacgttt tgcttggggc aattttcctt tgtactaccg gtgcagaggc
      121 tttatattca gaccttgggc attgtggtgc ggccaatatc cgcatttcat gggtgtatgt
      181 aaaatctacc ctcatcatca attacctggg acagggagct tgggttctca atcatcaggg
      241 cttttcagga aacccgtttt ttgctattat gcccagttgg tttctgctta tcggggttgc
      301 tatggctacc cttgcggcta tcattgccag tcaggctttg atttcaggct catttacgct
      361 tattagtgaa gctatttcgc tcaatttctg gcccaaactg aaaaagattt atccctccaa
      421 tgtcaaaggg caagtgtata tccctgctgt caattggttt ctgcttgcag gttgtatcct
      481 ggttatcctt atttttcaaa aatcatcgaa gatggaagcg gcctatggat tgtcaataac
      541 cataaccatg ctgatgacaa ctactttgct tttattttac ttatattcaa aacatataca
      601 ttttgggctc atagtgcttg ttgggcttgt gtatgttttt atcgagtcta attttctgat
      661 tgccaactta tacaagttta ttcatggagg atggtttacc atcttgttgg ctacactgtt
      721 ttttctggtc atgtatggat ggtatagtgc ccgtaaaata cgcaaccgct atcttacctt
      781 tgacaagctc gatgattaca tgccggtaat agctgaaatg agcaaagaca ataagatccc
      841 caaatattcc actcatctgg cctttttaac acacgccaac cgtaaagatg aaattgagaa
      901 aaaaattatt ttttctattt taaataaaaa gcccaaacgg gctgatgtat attggttttt
      961 gcatgtgaat atccttgatg atcctgatac tttcgaatac gatgtgcaaa ctatcgttcc
     1021 gggtatagcg tataaggtca attttcacct tggtttcaaa atagagccgc gtattaatat
     1081 gtatttcaac caagtgttga acgaaatgaa ggaacacaaa gagatagatt tgaccagcaa
     1141 gtacaattcg cttaaaaagt ataacattga tgccgatttt gcttttataa tagttgagcg
     1201 tatatttaac ttcgacatgg acttttcttt acatgataaa attattgttg gattccacaa
     1261 atttttgaaa taccttggta tctctgaagc ttatgcttat ggattggata tgagcaacat
     1321 tgttacagag aaagtgccca ttaatagcat tcaccaggta aaaagaaaaa tccacaggat
     1381 taaaagaata taaaaatggc caaaaccaag accagatatg tatgccaatc atgtggcgcc
     1441 gagtcttcaa agtggcttgg ccgctgccct tcttgcaacg aatggaattc atttgttgaa
     1501 gaagtggtgg caaaagaggt ttcgcgccta tcggacaggc ctgtctatgc agcatcaaca
     1561 cctataccta ttggaaaagt tgacttcacc gaacgtaaac gtattgatac tacctttcgt
     1621 gaggtaaacc ggaccctcgg aggaggttta gtgcctggtt cgatgattct gcttggcggt
     1681 gagccgggta ttggcaagtc aacccttgct ttgcaattgg ccttgaaaat gagagatatt
     1741 aaaacccttt atatctctgg cgaagaaagc gccgaacaaa ttaaactgcg ggctattcgt
     1801 cttggcgacg gcaacgacaa ctgtcacctc ctttgcgaaa catcgttgga aaccattctt
     1861 tctactctcg agcagtttgc cccgcaaatg gtcattattg actcaataca aactttgcag
     1921 gccgagtata ttgaatcatc gccgggaagc gtttcgcaaa tccgcgaatg ctcttcacgc
     1981 ttgttgcgct atgcaaaaac ttcggccgtt cctgttattc tcattggcca tattgtaaaa
     2041 gacggttcac tggccgggcc aaaagtactt gaacacatgg tggacacggt gttgcagttc
     2101 gaaggtgaca gacaatactt tttccgtatt ttacgctcga gcaaaaaccg ctttggttca
     2161 acctcagaga ttggcatttt cgaaatgcaa caaagtggtt tggttgaaat attaaaccca
     2221 tcggaaatac tcatgtcgca tagccagcag gcattgagcg gattggctat agccgcagca
     2281 cttaatggta tgcggccata tatgatagaa actcaggcat tggtaagctc tgcagtgtat
     2341 ggcacccctc aaaggtcagg tacaggcttt gattttcgca ggctcaatat gttgttggca
     2401 gtattagaaa agcgtgcagg gttcagattg ggggctaaag acgtgtttct caatatttcc
     2461 gggggtatta aagtagatga cccggcaata gacctcgctg ttgctgctgc tatactttcg
     2521 tcaaatgcag acgttgccat tcctcaaaaa acatgccttt gcggcgaaat aagcctttcg
     2581 ggcgaagtta ggcctgttta ccgcattgaa caacgtattg ctgaggctga aaaattaggg
     2641 tttgaacgta tcatcctggc gcattcggct gcaaaaaata atatcagcac caaacatata
     2701 aaaactgttt atatctcgaa aattgaacaa ctgcctaagt tgctgtttgg gtaaggtttt
     2761 tcaaaatagc cctaaatgcc cgccaaaaac aaaaactgcg atagcaacag ctgcaatggc
     2821 agctatcaat acagctccag cagccatatc ttttacgtgc attgcattat cgtgtttttc
     2881 gggatacatt ccgtcggtta gcttttcaat ggccgaatta aaagcctcgg cagctataac
     2941 caccccaatg gcaaggcata caagagccca ttctaatgcc cggagttgaa aataaatacc
     3001 agcagaaata acaacaatag ttgccaacaa atggatttga gcattggctt catgcctgaa
     3061 aagcctgtaa ataccggcaa aagcatagcc aaagcttttt attcgtttaa caatccagtt
     3121 catattcttc ttctcctttt ttctttactc ttggctcttg attcttgact ctattctaat
     3181 accatttcaa tatgcgcctg ttcttaaggt attttttgtg aaaccataga ttaagcttgt
     3241 atgtagcata agcgctggca gtcccaagta ctgctcctgc taaaacatct gaagggtaat
     3301 gcacgccaag gtgtatacgc gaataaccta ccgatgtggc ccaaacataa gatggcgcta
     3361 ttacatacca ctttggataa gccaaactta gcgatgtcgc cgtcgagaaa gctgctgttg
     3421 tatgacccga aggaaatgaa gggtcgccac catccaattt tttgcatata taatcgtatg
     3481 tttcaaatgg cctttttcgt tttactgtat atttcagggc ataagcaaaa ccctcggcca
     3541 ctgccaatga agctccgata gcgattgctt ttgacaacat tgcactgtcg cgttttacga
     3601 gggctgttgc ccctatgcat aacggaaccc caacaacagc ataggtcgta gaattagaaa
     3661 taaacttcat cgccgggtct gctttcaact cgtttggaga gttaagcctg tcaaggattt
     3721 tgacatctat attttgagaa tatgaaaggg aaaaaatgcc caaagaaaat accagaaatg
     3781 aaataaagcg atgcataatt tttatttttc tgggacaaag atagaggatt tgattacata
     3841 gaaaagattg tttttggtta catcttgggt cgctcttatg gagcttaatt aagagccgga
     3901 gaaagctttc tataaacagc aagctcctac ggagctaaag gccgtttagc taatctttca
     3961 gcacaaaatt aatatttcat attttgcccc atcggggcat cctgtttata aaaattgcaa
     4021 caattactat aggttagctc cgtaggagcg tcctgaaaaa accataaaat ccatgaatca
     4081 ttgaaaaatt ttgtatattt cctccccaaa ccccaaatct atggcaaata catacaccca
     4141 aatttatctg caattagtat tttctactct tggcagggaa aatataatac cacaaaaaca
     4201 taaagaggaa ttacataagt atgcctctgg aattatcgaa aatagaaacc ataagttatt
     4261 agctattaat tgtatgcctg atcatgtgca tatattgata ggctataagc ctgctcaacc
     4321 tttgccagat ttgatgcgag atataaaagc tgtgacatca ggttttatta atgataaaaa
     4381 atggcttcct ggaaaatttc ggtggcaaga aggctacgga gctttttctt actctcattc
     4441 tcagattgga gatgtgataa aatatatcaa taatcaagaa tcgcatcaca agaagatatc
     4501 tttcaaagaa gaatatcttg tgtttcttaa taaatatcaa gtggaataca gtgaacaatt
     4561 tttgtttgaa ttttatgaat agggctcccg actttgacgg tttgatttta taaaacaaag
     4621 atattcaaat agttattat
//
LOCUS       DAOSPF010000128         4460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_19590_length_4460_cov_7.194325, whole genome shotgun sequence.
ACCESSION   DAOSPF010000128 DAOSPF010000000
VERSION     DAOSPF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19590_length_4460_cov_7.194325"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..550)
                     /locus_tag="PK252_14480"
     CDS             complement(200..550)
                     /locus_tag="PK252_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15961.1"
                     /translation="MKTIEVAVIRDVADYENALKRIREIFNAEPGSDEFYELKMLSME
                     VESYEEENISFPDVDPIDIIEFVLKQHGLKQNALVGILGDKTTVSKVMNRKRPLTLDM
                     IRNFCTRFRVPVEA"
     gene            complement(587..889)
                     /locus_tag="PK252_14485"
     CDS             complement(587..889)
                     /locus_tag="PK252_14485"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HOT15962.1"
                     /translation="MRIIAQETLAKFWRKHPDSAVPLGSWLKLAKESKWKNSNDVKEI
                     YPYVSVLSNNRVVFNIKGNDYRLVVAFAFNAGVGYICFVGTHQEYDKIDANTIWDY"
     gene            complement(1380..1655)
                     /locus_tag="PK252_14490"
     CDS             complement(1380..1655)
                     /locus_tag="PK252_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT15963.1"
                     /translation="MSKEPIEYLKHIRDESFYILSVITPDKTKDDFLVDETLKRAVIR
                     SLEIIGEATKKIPADFKIKWSRIKWKDMAGMRDRLIHDYMGVNYSIV"
     gene            complement(1648..1944)
                     /locus_tag="PK252_14495"
     CDS             complement(1648..1944)
                     /locus_tag="PK252_14495"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT15964.1"
                     /translation="MTTKEFILSTLKSKEKEISKYGIRAIGLFGSYVRDEQSENSDID
                     ILVDFEPDKENFDNYMAVYDMIEKMFKNQRVEIVTKNGLSPYIGPKILNEVKYV"
     gene            2606..2941
                     /locus_tag="PK252_14500"
     CDS             2606..2941
                     /locus_tag="PK252_14500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT15965.1"
                     /translation="MTLGEHIMLLRKQKEFSQAALGKAIGTSGDIIGRYERNIMTPSI
                     EVIVKIADTLGVSIDYLVGKTNIELDRNTLKRLEDIATLSDENKLFIFRMIDMALRDL
                     KTEQAYATK"
     gene            2954..3175
                     /locus_tag="PK252_14505"
     CDS             2954..3175
                     /locus_tag="PK252_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15966.1"
                     /translation="MNLQYITDTKGHKSAVQLPLKDWEQIQKDLDELERLRNKKLFMI
                     ELAEAVEEMKLIKEGKKQARNAEDFLNEL"
     gene            3165..3491
                     /locus_tag="PK252_14510"
     CDS             3165..3491
                     /locus_tag="PK252_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT15967.1"
                     /translation="MSFNVKTISVFERQAKRLMKKFPSLKKEIQELIKELKEEPEKGT
                     SIGHNCYKIRLAIASKGKGKSGGARVITHLVFKDDTVYLLTIYDKSDIENLTDKEILE
                     LIKLIP"
     gene            complement(3954..>4460)
                     /locus_tag="PK252_14515"
     CDS             complement(3954..>4460)
                     /locus_tag="PK252_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15968.1"
                     /translation="NINIYYTPLIAGNGPSGGFVLLSSHPAPQGGGGNGGLLNDGASF
                     GTAVAGSITTMVQGSAELTQADALAKINRANRVIASEKAAFKTMGKLATGAKFLGSAL
                     GVVSIADHSMKAWNSFSNEGIYSVNGWLNVGKVGIDGALMIMKSNPVGLTISVGYGVL
                     DASGYLDY"
BASE COUNT         1416 a          903 c          777 g         1364 t
ORIGIN      
        1 tggcccatgt accaaagata ggcattttat tgcacctggt caaggctttg gcttagacgc
       61 ttagttttaa cacccgaaag cagttgcaaa ctgcaaagaa aggaagaagt ccaagccgca
      121 gacttgaact attgccttcg ggcaaacttg cgacatagga gctaaataaa ttaggctaca
      181 agcaacttat actcttgaat caggcctcta caggtacacg aaagcgtgtg caaaaattac
      241 gtatcatatc gagggttaaa ggtctttttc tattcataac cttcgaaaca gtagtcttat
      301 cacctaaaat accaactaag gcattttgct ttagtccgtg ttgtttcaaa acgaattcga
      361 ttatatcaat ggggtcaaca tctggaaagc tgatgttttc ttcctcatag ctttcaactt
      421 ccattgaaag cattttaagt tcataaaact catcggaacc tggctctgcg ttaaatatct
      481 cacgtattct tttgagagca ttttcatagt ctgcaacgtc ccttattaca gctacttcaa
      541 ttgtcttcat gataaattat tctctactat ctttagttta caatctctaa taatcccaaa
      601 tggtatttgc atcgattttg tcatactcct gatgagtacc cacaaaacat atatacccaa
      661 cccctgcatt aaacgcaaat gctacaacca acctgtaatc attccctttt atattaaaga
      721 ctactcgatt atttgaaaga acgctcacat acggataaat ttcctttaca tcattggagt
      781 ttttccattt ggattctttg gcaagcttta gccatgagcc taatggaacg gcgctatcag
      841 ggtgctttct ccaaaacttt gcaagggtct cctgagctat aatgcgcatt gacttaaact
      901 ttttacgaca aaaataataa aaagttggca atttgtcaat tttttaaagc atattatcta
      961 ttacctgaaa cctccgtact gtatggggca gtaagcatat actttcggcc catgtaccaa
     1021 agataggcat tttattgcac ctggtcaagg ctttggcttg cattggctac tcttggctac
     1081 aggctgcagc ctgatgaaat acttacggtc agaggctctg ccttagccga acggggggcc
     1141 gggctacaaa agctttgcgt ttctgaaaac acaaagcttt taggcctgct caaactaaac
     1201 cacgcaaagc cttgacacac catgttaaac ggccggaggc cgaatacaat gcgacgtagc
     1261 aggatgctac gccggaccgc ttttattggc aagctacgat gaacgggggc attttactca
     1321 ttttctatca cttcaattat ctgtgcatgt aattcaggaa ttttattttt aacaacgtcc
     1381 tatacaatcg aataattgac tcccatataa tcatgaatta acctgtctct cataccagcc
     1441 atatctttcc attttattct actccatttt attttaaaat ctgctggtat ctttttcgta
     1501 gcttcgccaa ttatttccaa actccttatt acagctcgtt ttaatgtttc atcaaccaaa
     1561 aaatcatctt ttgttttgtc aggtgtaatt actgaaagaa tgtagaaact ttcgtcacgg
     1621 atatgtttca ggtattcaat aggttcttta gacatacttc acctcgttta aaatctttgg
     1681 accaatataa gggctcaatc catttttagt aacaatctca acccgttgat ttttaaacat
     1741 tttctcaatc atgtcgtaaa ctgccatata gttgtcgaaa ttttctttat ccggttcaaa
     1801 atcaactaat atatcaatat cactattctc agattgctca tctcttacat atgacccaaa
     1861 taatccaata gctctgatcc catattttga aatctctttt tcctttgatt ttaatgtact
     1921 caatataaat tctttggttg tcatattcaa ttattattta tacaaatata ataatctttt
     1981 gtctgcgtgc atgttatgtc acttaaaaac accaacaact ttagctattg ttgaaacagc
     2041 aagcgtcaca aacgagctag cagggttata aggggctaaa ccagccgcat agcatctcgt
     2101 ggccggtgtg cccgcagtcg aagcggggct gcatggccgg catggcggtg tgcggggcag
     2161 tatgcatgta cttttggccc atgtaccaaa gataggcatt ttattgcacc tggtcaaggc
     2221 tttggcttac attggctact cttggctaca ggctgcagcc tgatgatgta atacggccag
     2281 aggccgaata caatgcgacg tagcaggatg ctacgccgaa ccgcttttat tggtgagcta
     2341 cgatgaacga aggcagttca tacgcatatg cttatctggc tttaacccat acttggcaag
     2401 tacctgtgcc atcgttaaac gctgctttat ttcttggatt tccatgcaat aaaaaatttt
     2461 tttaaattct tttttactac ctattcgtcg taaatgtaat ttattaacct ttacaaacca
     2521 aattttaaag gttttgtttt gtacgtttaa gtagtgtata tttgcaaaaa caaacaataa
     2581 ttagtctaat acaatacttt taggtatgac tcttggcgaa catataatgc ttttacgcaa
     2641 gcaaaaagag ttttctcagg ctgctttggg taaagcaatc ggcacatcag gcgatattat
     2701 cggaaggtac gaacgtaata ttatgacccc atctattgaa gtgattgtta agattgccga
     2761 tactttaggg gtttcgattg attacctggt aggaaaaacc aatattgaac tggacaggaa
     2821 cactttaaag cggcttgaag atattgccac actttcggac gaaaacaaat tgtttatatt
     2881 ccgtatgatt gatatggctt tgcgggattt aaaaaccgaa caggcttatg caacaaaata
     2941 ataaactaaa attatgaact tacaatatat tacagacaca aaagggcata aaagcgccgt
     3001 gcagcttcct ttgaaggatt gggagcagat acaaaaagac cttgacgaac tggagcgttt
     3061 aaggaacaaa aaactgttca tgatcgagct ggccgaagct gttgaagaaa tgaagctgat
     3121 taaagagggc aaaaaacaag cccgtaacgc cgaagatttc ctcaatgagc tttaatgtta
     3181 aaaccatatc cgtttttgaa cgccaggcca aacgcctgat gaaaaaattc ccgtcgctca
     3241 aaaaggaaat acaggaactt ataaaagaac tcaaggaaga acccgaaaaa ggcacttcca
     3301 ttggccataa ctgctataag atacgccttg ccattgccag taagggtaaa ggaaaatcag
     3361 gcggggcaag ggttattacc catcttgttt ttaaggacga taccgtttac cttcttacca
     3421 tttacgacaa atcggacatt gaaaacctta ccgataaaga aattctggaa cttattaaac
     3481 ttattccata aaaaagcccg gaactgccgg gcttttgtgt tattagaata aggctggaga
     3541 tatcatcgaa aagaaaaaca atagaacact gactgttaaa taaattacta caaataatcc
     3601 tttttgcttt cgttcttgaa tactctcagt tctaaagttc ttaatagtgg acattatctt
     3661 ttctttattt tttctataaa agataaatag cacaatgtaa ataggggcta ttaataataa
     3721 agggaatagt acaaacctat ccataattcc attgtctatt cttattgcac ttacaaaatc
     3781 tctgttgaaa atagtctgaa caaaaagtaa aagcgtcaaa atatgaataa ggaaaatagc
     3841 tccaactaat ataaaagcat gtatccaact atctgaggag tttttatatt tataaagact
     3901 tttaaaacga taaaagatat ataaaaagaa attcataact tatttaatta aggttagtaa
     3961 tctaaatagc cagatgcatc taaaacacca tagccaacgg aaatagttaa accaacaggg
     4021 tttgatttca taatcatcaa tgctccatca ataccaactt tgccaacatt gagccaacca
     4081 ttaacgctat aaataccttc attagaaaat gagttccatg ctttcataga atggtctgct
     4141 attgaaacaa cacctaatgc actacctaaa aacttagccc cagtagcaag cttccccata
     4201 gttttaaatg ctgctttttc agaagctatg actctatttg ctcgatttat ttttgctaat
     4261 gcatctgctt gtgtaagttc tgctgaacct tgaaccatag ttgttattga acctgctaca
     4321 gcggtaccaa aagaagctcc atcgtttaga agtccaccat tgccccctcc tccttgggga
     4381 gcaggatgtg aacttaatag cacaaaaccg ccagaaggcc cattaccagc aatcaaaggg
     4441 gtatagtata tattaatgtt
//
LOCUS       DAOSPF010000129         4349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_20221_length_4349_cov_6.832091, whole genome shotgun sequence.
ACCESSION   DAOSPF010000129 DAOSPF010000000
VERSION     DAOSPF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20221_length_4349_cov_6.832091"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..221)
                     /locus_tag="PK252_14520"
     CDS             complement(12..221)
                     /locus_tag="PK252_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15969.1"
                     /translation="MSPQELQERIKQLEAENFQLKQAVEMQDNKHAISCMSLINSSED
                     VILAIDAVNFNILIYNSGMGEIPRL"
     gene            342..803
                     /locus_tag="PK252_14525"
     CDS             342..803
                     /locus_tag="PK252_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT15970.1"
                     /translation="MLKFTSSVVFVSNINVSKDFYIQLLKQEIELDFGTNISFKSGIS
                     LWQVNQAHVIAQELNTSSNNNRFELYFESDQIEKDFEELKSRGVKFLHEINEEPWGQQ
                     TIRFFDPDGHLVEIGEPMEVFVRNMKKKGLTAKQIAAKSGISIETINSILK"
     gene            complement(809..1846)
                     /gene="porQ"
                     /locus_tag="PK252_14530"
     CDS             complement(809..1846)
                     /gene="porQ"
                     /locus_tag="PK252_14530"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOT15971.1"
                     /translation="MSLKSLLLTCSFIVAVYNVGFTQTGGVGTYKFLEKAGSARSASL
                     GEKNISVSDGDLNMVANNPALLTESSASKLALNYLSYYADISMGYAAYSLKQNSKASL
                     AAGIQYINYGSFDGRDVVGNATSSFNATDMALNIFYSRPIDSAFHIGVTVKPLLSYYE
                     KYSSMGIAFDAGATYTMDEGRLTFAAVIRNVGSQIKPYVSGKYEDLPFEAMLGVSQRL
                     HHAPFRISLTLHSLQRYEMTYDDVFENESSFGESASDSKAEKFMENVLRHAIIGVEFL
                     PSKSLFINGSFNYQRRKEMMLLDAPGATGFAFGGGLNLRRFSFCYSYSQYFVSGGAHT
                     FSLQLNLAPQE"
     gene            complement(1903..2982)
                     /locus_tag="PK252_14535"
     CDS             complement(1903..2982)
                     /locus_tag="PK252_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOT15972.1"
                     /translation="MKFLFIVQGEGRGHMTQAISLADILRAEGHEVVHVLTGKSKRRE
                     LPAFFFQKIKSEVTLFESPNFVIDAKSKKISLWKTIVYNVFKLGTFIKSLRQIHRIVK
                     TTKPDVIINFYDLLSGLYSLTYRPKQKYICIGHQFLLNHPDFTFPKGYAVDKILMKIN
                     TAFTSIRADRHLALSFRPMPDVPDKKIYVVPPLLRVEITRLLVENKKYILGYLLNAEY
                     GLEIENWHKNHPELELHFFWDKKDVPEEYTVHPNLTFHKLNDVKFLEMMSKCGAYCST
                     AGFESICEAMYLGKPIMMVPSYGHFEQACNALDATLAGAGIQNVVFNLDPFIEYIPNH
                     KDISGEFRKWADSSRRKFIELLTSN"
     gene            complement(3121..3927)
                     /locus_tag="PK252_14540"
     CDS             complement(3121..3927)
                     /locus_tag="PK252_14540"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOT15973.1"
                     /translation="MNKDDEPRKAYKTIVLSDIHLGASESKAKELVRFLKRHTCETLI
                     LNGDIIDGWQLRRGGDWKKKHTRFFRVVLKMTEKQNTEVIYVRGNHDDFLDNVLPFSI
                     GNFSIVRDYIYVSHGKKYFVLHGDVFDSITTNMKWLAKLGDIGYTLLLKINKIYNNYR
                     IKRGKPYYSLSREIKAKVKSAVSYIGDYEKELVDLAQSKNYAGVICGHIHHPAITQYD
                     NFVYMNSGDWVESLSALVEDFDGKWSIVTYEQFVKEINEAENNDKIQDTE"
BASE COUNT         1384 a          858 c          720 g         1387 t
ORIGIN      
        1 aaatgcttcg cctaaaggcg agggatttca cccatcccag aattataaat taaaatgttg
       61 aaatttactg catctatggc taatattaca tcctcactgc tgtttatcaa agacatacaa
      121 ctgatagcat gcttattatc ctgcatctca acagcctgtt ttagctgaaa gttttcagct
      181 tccaattgct ttatccgttc ttgcagttct tgggggctca taagcagagg catttttatg
      241 tttgcaagta aagcttatag cgctgcaaat aagcttactt ataaagttaa gaaaaaataa
      301 ataaatagcc taaattagca gaattcatca atgccattaa aatgttaaaa ttcacttctt
      361 ctgtagtctt tgtcagcaac ataaacgttt caaaagactt ttatattcaa cttcttaagc
      421 aagagattga acttgatttt ggaacaaata tttcatttaa atctggtata agcctctggc
      481 aggtcaatca agcccatgtt attgcccaag aactaaatac ttcgtccaat aataacaggt
      541 ttgaactata ttttgagtcc gaccagatag aaaaagactt tgaagagcta aaatctcgcg
      601 gggtaaaatt tcttcacgaa ataaatgagg aaccttgggg gcagcaaacc atacgttttt
      661 tcgaccccga tggacatctt gtagaaattg gggaaccaat ggaagtgttt gttagaaaca
      721 tgaagaaaaa agggcttaca gcaaaacaaa tagccgcaaa gagcggtatc tctattgaaa
      781 ctattaacag tattctgaaa tgataaaact attcttgagg agcaagattt aactgaaggg
      841 aaaacgtatg tgcccctcct gaaacaaaat attgtgaata agaataacaa aaagaaaaac
      901 gacgcaaatt taatcctcct ccaaaagcaa atcctgtagc cccgggagca tctaaaagca
      961 tcatttcttt tcggcgttga taattgaacg aaccattgat gaacaggctt tttgaaggca
     1021 aaaattcaac cccgatgatg gcatgcctga gtacattctc cataaatttc tctgccttgc
     1081 tatccgacgc agattctcca aaagagctct cattctcaaa tacatcgtca taagtcattt
     1141 catagcgttg caaactgtgc agtgtcaatg atattctgaa aggcgcatga tgaaggcgtt
     1201 gcgatactcc tagcatagct tcaaatggca aatcttcata cttacccgaa acataaggct
     1261 ttatttgtga cccaacattg cgtataactg cagcaaaagt aagccttcct tcgtccatag
     1321 tgtatgtagc tcctgcatca aatgcaattc ccatagatga gtatttttca tagtatgaca
     1381 acaaaggttt aacggttaca cctatatgaa aagcactgtc tatcggcctc gaataaaaaa
     1441 tgttcaatgc catgtcagta gcattaaagg agcttgttgc gttgcctact acgtcgcgcc
     1501 catcaaacga gccataattg atatactgaa tacctgctgc caatgaggct tttgagtttt
     1561 gtttgagcga ataagctgca taacccatgc ttatgtccgc atagtaactc aaataattca
     1621 atgcaagctt ggatgcagaa ctttctgtta ataaagcagg gttattggca accatattta
     1681 aatcgccatc agaaaccgaa atgtttttct cacccaaaga tgctgaacgt gcagagcctg
     1741 ctttttcaag aaatttgtat gtccctaccc ctccggtttg tgtaaaccca acattgtaaa
     1801 cagctacaat gaaagaacat gtaagtaata aactttttaa ggacatacca atgattattt
     1861 attcctgaaa acggccaaat ttaaaaaata ttatccaata tcttaatttg aagtaagcag
     1921 ctcaatgaac tttctgcgcg agctgtcagc ccattttctg aattcacccg atatgtcttt
     1981 atgattggga atatactcaa taaaagggtc aagattaaaa acaacattct gaatacctgc
     2041 accagcaagg gttgcgtcaa gtgcgttaca agcctgctca aagtgtccgt atgaaggcac
     2101 catcataatt ggctttccca ggtacatagc ttcacatatt gactcaaacc cggcggtact
     2161 acaatatgca ccgcatttgc tcatcatctc caaaaatttc acatcattca gcttatgaaa
     2221 tgtaaggttt ggatgaactg tatattcttc cggaacatct tttttatccc aaaagaaatg
     2281 aagctcaagc tccgggtggt ttttatgcca gttttctatt tctaagccat actcggcatt
     2341 caaaagatag ccaagtatat actttttatt ttcgaccaac aaccttgtaa tctcaaccct
     2401 gagcagtggc gggacaacat atatcttttt atcgggtaca tctggcatag gcctgaaaga
     2461 aagcgccaaa tgcctgtcag ccctgataga ggtaaatgct gtatttattt tcatcaggat
     2521 tttatccaca gcatagcctt tagggaaggt aaaatcggga tggttcaata aaaactggtg
     2581 acctatgcaa atgtattttt gctttggcct gtatgtcaat gaataaaggc cagacaaaag
     2641 atcgtagaaa ttaataataa catcgggttt tgtagttttt acaatccgat ggatctgtct
     2701 caaacttttg ataaaagtcc ctaatttgaa tacgttataa acaattgttt tccaaaggct
     2761 tatttttttt gatttagcat caataacaaa attagggctt tcaaataagg taacttcaga
     2821 cttgattttt tgaaaaaaaa atgcaggtaa ttctctcctt ttactttttc cggtaagtac
     2881 atgaactacc tcatggccct cggcccttaa tatatctgct aaagaaatag cctgggtcat
     2941 gtgccctctt ccttctccct gtacaataaa taaaaacttc atgttaaata atcaagatag
     3001 ttttgtttca atagtattat ttacagggga caagtttaat aattttattt acaccttggg
     3061 cattttagcc aataattaaa tcaccattaa aaagcttttt atcaacttat tcagattttt
     3121 tcactctgta tcttgaattt tatcattatt ctcagcttca ttaatttcct ttacaaattg
     3181 ttcataagtc acaatgctcc atttgccatc aaaatcttct actaaagcac ttagagactc
     3241 tacccagtct ccagagttca tgtaaacaaa attatcgtat tgtgtaatcg ccggatggtg
     3301 aatatgccca caaatcacgc cagcataatt tttcgactgg gcaaggtcaa ccagttcctt
     3361 ttcataatca cctatatacg aaacagcaga ttttaccttg gcctttattt ctcttgacaa
     3421 cgaataataa ggctttcctc gcttgattcg ataattatta tatattttat taattttgag
     3481 caaaagggta taacctatat cccctaattt ggcaagccat ttcatattag tagtaatgct
     3541 gtcaaataca tcgccatgaa gcacaaagta cttcttaccg tggctcacat aaatataatc
     3601 ccttactata gagaaatttc caatactgaa aggcaaaaca ttatctaaaa aatcatcgtg
     3661 gttgcccctg acgtaaataa cctcggtatt ttgtttttcg gtcattttca atacaacgcg
     3721 aaaaaaacgg gtgtgtttct ttttccaatc acctccgcgt cttaattgcc aaccatctat
     3781 aatgtcacca tttaagatta acgtttcgca agtatgcctt ttcaaaaaac gtactaactc
     3841 cttcgctttt gactctgaag cccctaaatg tatgtccgat agaacaatgg ttttatatgc
     3901 tttccttggt tcatcatctt tgttcatatt tattgttttt ggtttatctc aaatttaggt
     3961 attttttaaa taattgggtt tacggcttta ttatattaac attaccataa tgttaaatac
     4021 ccctttaaca ggctgtttag gtgattagta aaataaaaaa tcattgttgt ccggtaaaaa
     4081 caggtcgctg ctatgctgct tagaaacatt tggtgtattt ggttatccta caaacagttc
     4141 gcagctatgc tgctattatt tagtcgctta gcgatgtcct gtttgtagta aaattaaaca
     4201 tctcttaaat caagccccgg cggggcaacc agtttctatt caacgatatt actggtattt
     4261 ataagtatct attttattat accgggcaac agtgatttta aatagcaaaa aacttgatag
     4321 ttacgggtta cttttttatc tttagtgac
//
LOCUS       DAOSPF010000130         4331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_20347_length_4331_cov_5.694107, whole genome shotgun sequence.
ACCESSION   DAOSPF010000130 DAOSPF010000000
VERSION     DAOSPF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20347_length_4331_cov_5.694107"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PK252_14545"
     CDS             complement(<1..1215)
                     /locus_tag="PK252_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT15974.1"
                     /translation="MKTIFTILLFVAFAFTAKAMVSNSVQVDNAGNLRREILNTFGDT
                     KNIHTLIITGFLDARDFQFMRDSLPKLSELDIESVTINEYSGLNGTDPQISFYAANEL
                     PAFSFHNSYTGNSKVLLKVLKLPASITSIGYKALAGCTGFNDSIFIPSLVSSFSNDAF
                     EGCSKLERFSVSPQNLYFTDIDGIIYSKDKKKLILCPLNKAANPLILPSGLEHITDYA
                     FFNCTGIKGDLIFPNSLKTIGKWAFAYCSGLKKVIFPDSLTEIGESAFSECSGLKDGL
                     VIPSLITEIKDFTFFGCINFKDSLILPNSLKKIGNGAFYNCQGLSGSLKLPNAVISIG
                     AEAFYNCKSFTGTLYIPDNTKSIGARAFKNAKNITIVKLHQTTPPKIASETFDISCQI
                     RVPSAAITIYKAA"
     gene            1492..2511
                     /locus_tag="PK252_14550"
     CDS             1492..2511
                     /locus_tag="PK252_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT15975.1"
                     /translation="MNKRVKISSVLLVVFMLLNSCIQESEKMKIALMLPTLNISRFHK
                     DSVFFVKEAEKLGCEGLVVSADNNEQLQFDQAKELIDKGAKVLIIAAVNSNTAGLMVR
                     MAIDKGVKTISYDGVLNNCDVDYCISFDNEKIGELMAQYAVSKAPQGEYVILGGDKSN
                     LNAIQIRQGQEKVLAPYIAQGKIKVTFDTYVENWNRDEAYMIVKRYLNGLCGKVPAAV
                     IGANDEIGLGAIKAVEEYYHGESVVLPVVTGQDASVDGCVSIVEGKQSMTVYKPIIQL
                     ASTAAEVAVKLAKGQKIEGINATTNNGYKDIPTILINLISVDKQNIESTVIADGFLNK
                     ADVYK"
     gene            3106..4185
                     /locus_tag="PK252_14555"
     CDS             3106..4185
                     /locus_tag="PK252_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT15976.1"
                     /translation="MHPTHLKLLKTFHPSRIIIAMLIGVAVVLLIFMRDFKPGTFDTL
                     NFSFFSFLFIFLALLCMAARDIGYMIRIRVLSDNQLSWWQSFRIVMLWEFTSAITPSA
                     IGGTSIAILFVTKEGISVGRSTSIVLATSFLDEMYFILLFPLLLIFVLPSDLFSTPNT
                     TGNGINWTNEFFWFAIIGYSLKLAYVILVIYGMFFKPRAFKWLLLKVFKLPFLRRWRV
                     GAAKAGDDMITSSLELKRKPFTFWIKSFMATFMSWTSRYLVINMLFLAFFVVNDHFLL
                     YARQLVMWIMMLVSPTPGGSGFAEYIFSRYLAEFMPSSAVSIGALAVALAFLWRLITY
                     YPYLIIGGFIIPGWIRSKFMHEKNN"
BASE COUNT         1356 a          666 c          898 g         1411 t
ORIGIN      
        1 agctgctttg tatatggtaa tagcagctga aggcactctt atttggcagg agatatcgaa
       61 tgtttctgag gcaattttgg gaggtgttgt ttggtgcaat ttaactatag taatgttttt
      121 tgcattttta aaagcccttg caccaataga ttttgtattg tcggggatat acaaggtacc
      181 tgtaaagctt ttgcaattgt aaaaggcttc ggctccgata gagataacag cgttgggcaa
      241 ttttaaactt ccactcaaac cctgacagtt ataaaaagca ccattgccaa ttttttttaa
      301 tgaattgggg agtatcaggc tgtctttaaa gtttatgcac ccaaaaaagg taaagtcttt
      361 gatttctgtt atcagagaag gaataaccag cccgtctttc agtccactgc attcagagaa
      421 agcactttct ccaatctctg ttaaagaatc agggaaaata acctttttta gccccgaaca
      481 gtaagcaaag gcccatttgc ctatggtttt aagtgagttt gggaatatta aatctccttt
      541 tatcccggta cagttaaaga atgcgtaatc ggttatatgt tcaagcccgg aaggcaatat
      601 taaagggttg gcagccttat ttagagggca aagtatcagt tttttcttat cttttgaata
      661 aataatgccg tcaatgtcag taaaatatag gttttggggg ctgacagaaa agcgttcgag
      721 tttactacaa ccttcaaatg cgtcattaga aaatgaagaa actaaagaag ggataaatat
      781 actgtcatta aagcctgtac agcctgccag tgctttgtaa cctattgaag taattgaggc
      841 cggaagtttg agtactttta aaagtacttt tgagttgcct gtataagaat tgtgaaacga
      901 gaaagcaggt agttcattgg ctgcataaaa acttatttga gggtcggtac cgttaaggcc
      961 actgtattca ttgatagtta cagactcaat gtcaagttca gacagtttag gaaggctatc
     1021 gcgcataaac tgaaaatccc ttgcatctaa aaagccagtt atgataagag tatgaatgtt
     1081 tttagtgtct ccaaaagtgt ttaaaatttc tcttctgaga ttcccggcat tatcaacctg
     1141 gacactattt gataccattg ccttagcagt aaaagcaaaa gctacaaata ataatatggt
     1201 aaaaatagtc ttcaatcctt caatggttta aaaaatataa atcatccatc cggattaaag
     1261 gtttataggt atgtttgttc ttgtttggca ggtacaaaat taatatcaaa caatgttaga
     1321 ttgctacttt ttaactgaat atttgacaaa catttttata tttgtatggt tttaaatata
     1381 gctaaacaac ttttttatat aaaaaacgtt taaaacaata aaaatgtaat atttttatat
     1441 taagaaagtt gtggtttaaa ttggcggggg gcaaatgtta aaacttggat tatgaataaa
     1501 agggtaaaaa ttagctcagt actgttggtt gtatttatgt tattgaattc atgtattcag
     1561 gaaagtgaaa aaatgaaaat agcattgatg ttgccaacgc ttaatatttc caggtttcat
     1621 aaagacagtg ttttttttgt aaaggaagca gagaagcttg ggtgtgaagg attggttgta
     1681 agcgctgata ataatgagca gttgcagttt gatcaagcta aagagcttat agataagggg
     1741 gctaaagtgt tgattatagc agccgttaac tcaaatactg cgggtttaat ggttagaatg
     1801 gccatagata aaggggtaaa gactatttcg tatgatgggg ttttgaacaa ttgcgatgtt
     1861 gattattgta tttcgtttga taatgaaaag attggcgagc tgatggctca atatgctgta
     1921 tctaaagctc ctcagggtga atatgtgatt ttgggaggag acaagtctaa tttaaatgct
     1981 attcagataa ggcagggaca agagaaggtt ttggctccat atatagctca aggcaagata
     2041 aaagtgactt ttgataccta tgtagaaaac tggaaccggg acgaagctta tatgattgtg
     2101 aaaaggtatc ttaatgggtt atgtggaaag gttccggcag ctgttatagg ggcaaatgac
     2161 gaaattggct taggggctat taaggctgtg gaagagtatt atcatggcga aagtgttgta
     2221 ttgcctgtag ttaccgggca ggatgcctct gtagatggat gtgtgagtat tgttgaaggt
     2281 aaacaaagta tgacggttta caaacctatt atacaattgg catcgacagc tgcagaagtg
     2341 gctgttaagc tggcaaaagg acagaagatt gaagggataa atgctactac gaataatgga
     2401 tataaggata tcccgacaat actgattaat cttatttcgg tagataagca aaacattgaa
     2461 tctacagtta tagctgatgg atttcttaat aaagcagatg tgtataagta aaatttacgg
     2521 gtattaagga atatcaaata ataggagata agttagttta atttaattaa gctatttaac
     2581 aattgggctt accttacctt tattcgcact caccagatac atcacaaaac aaactgtcaa
     2641 ggcagcttaa tgtttgtctg gcttaaggtg tttgtgctag tcagaaaagc tataataata
     2701 ggcactacaa gctgaatgta aatataaatg ctggctgtag tagagtttaa gatgttgtac
     2761 gcatcaattg ataaggaggc agttctgtac tgttttgcta aaaactatat aaaaaaggac
     2821 tgcataaacc caaataggca tttagaaaat acttaaatag actaaaaaat gtaattcagc
     2881 attttaatga attatttcaa gccattttta aatcagctcg tcttgtttat gacgcgatgg
     2941 ttaagccggc taaatcatat agttttgttt tagttggttg caaaagtggc atttttttta
     3001 agcattaata aggtaaaaaa acaaacattt aacattaaac actcaaagtc ttaaagaaaa
     3061 tgagctaact tgccacctgt tataaccaac agaaaagcga gaattatgca ccctacccat
     3121 ctgaagctgc ttaaaacatt tcatccatca cgtatcatta tagctatgct cataggggta
     3181 gccgttgtgc tgttgatatt tatgagagac ttcaaaccag gcacttttga cacgttaaat
     3241 tttagctttt tctccttcct attcattttc cttgcattgt tgtgtatggc agcaagggat
     3301 atcggttata tgatacgtat cagggtattg tcggacaatc aacttagttg gtggcaaagc
     3361 ttccgtattg tcatgctttg ggagtttact tctgctatta ctccttctgc tattggagga
     3421 acaagcattg ctattctctt tgtaaccaaa gaaggtattt cggtaggcag gagcacttct
     3481 atcgtgctgg ccacctcatt tttagacgaa atgtatttta ttctattatt tcccctgcta
     3541 ttgatatttg tacttccttc tgacctcttt tctactccaa ataccactgg aaatgggata
     3601 aactggacca acgaattttt ctggtttgcc attattggct atagcctaaa acttgcctat
     3661 gtgatattgg ttatttatgg catgtttttt aagccaagag ctttcaaatg gttactttta
     3721 aaggtattta aactaccatt cctgcgacgt tggcgagttg gtgcggcaaa ggctggggat
     3781 gatatgatca cttcctcact ggaactgaaa agaaagccat ttacattttg gatcaaatca
     3841 tttatggcca ctttcatgtc atggacatct cgttacctgg taattaacat gctttttctt
     3901 gctttctttg ttgtaaacga ccattttttg ctatatgccc gacaattagt tatgtggatt
     3961 atgatgctgg taagcccaac ccccggaggc agtggctttg ccgaatatat cttctctcgt
     4021 tacctggcgg aatttatgcc atcttcagca gtttctattg gtgcgttggc tgtagctttg
     4081 gcttttttgt ggcgacttat cacgtattac ccttacttaa tcattggagg gttcattatc
     4141 ccgggctgga taaggtcgaa gtttatgcac gaaaagaaca attagctaat ttgaaaatta
     4201 gctaattcgg gaatttgaaa ataaattgtt gagaatccta agtatcattg acttgaaaat
     4261 tcaaagagaa tataccttaa gtgtcaagtt tcacaaggtt gtttatgata acttaattgc
     4321 aaagataaaa c
//
LOCUS       DAOSPF010000131         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_20389_length_4324_cov_7.579527, whole genome shotgun sequence.
ACCESSION   DAOSPF010000131 DAOSPF010000000
VERSION     DAOSPF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20389_length_4324_cov_7.579527"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="PK252_14560"
     CDS             <1..2223
                     /locus_tag="PK252_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT15977.1"
                     /translation="EFAIEAGEINIWDIDLVGHNQLYSQSHGKVFGRHSEYVTIGEKM
                     WELMVHPEDLPEVKKKFAEHFRKQTKYFETECRIQNKDNTYKWMLVRGKIASMDKNNM
                     PQKYIGMFIDITSRKQTEEKLAVSEEKFRTIVQQLSDIIFIIDENLTVLYESPSVSKT
                     FGYEPGYFLGKNGLSLIHRDEINIAKKEFEHLLQHNTDFKPTELRISHKDGYWVTLEI
                     LGDNLTSHPAIKGILLTARDISERKENEKQLELYSNHLEKLVKKRTEEIENINAELIA
                     INEELKAANEELYYKNEKLNEEIKIRIETQLMLEESENKFRSFIEQSTEGIVLIDEQG
                     RIVDWNKGMQSIFRIAKEDVINTFIWEFDYKFLPDKRKTAQQFEELKNSTIDYFSNLD
                     KSKVMTVEGIYQSIELKQKYLNVTIFPVITPKRRYLGRITRDVTYIHRAQEEIEKQSE
                     ELLQINQNLEEQKVQLEKALVELRKTQGQLIHSEKMASLGVLTAGVAHEINNPVNYIN
                     AALEGLKITLHDLSQIFEQYDSLSVENSANKLKEIDQLKTELDYPMLQDGVKILLNNM
                     QTGIARITEIVRSLRTFARIDENELRYSNIHELIDTTLIMLHNQYKNRIEIVKNYCNF
                     QNLNCYSGKLSQVFMNILSNAIQAIPEKGTIFITTNVNKDKEDFSISIRDNGIGMPAY
                     LKEKIFEPFFTTKEAGKGTGLGLSITYGIVQQHQGHIEVNSKQGQGTEFIVTIPTNLK
                     "
     gene            2230..3825
                     /locus_tag="PK252_14565"
     CDS             2230..3825
                     /locus_tag="PK252_14565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15978.1"
                     /translation="MANSNKEPILYVDDESENLQGFEYLLRRDFEVYVASSAKEGLEI
                     LQNTEIKVLLTDQRMPEMTGIEFLEQAIKIAPETIRIVVTAYSDSDTVMQSINQGKAY
                     HFITKPWNNAELKNVIRRAVETFNLRRDKIELITHLQNVNQELFQAKQRAEEADRLKS
                     SFLANMSHEIRTPLNAIVGFSNLLVNELPAEDEMHRQFQQIIESSSNDLLNIIEDILD
                     TSQIESGFVTIRPTVIEINQFMVDSLVAFQNHPQVKEKKLEIRYNYPKIEEKLTYFVD
                     AIRLKQVLNNIMGNAIKFTEKGYIEIGYKFTNEEKNYIQFYIKDSGIGIPNDKYEYIF
                     ERFRKIESSTADTLYRGNGLGLYISKRLAQMMGGDIIVESELDKGSTFTIHLPFYTKS
                     PEELKTISHSLNISKIAWPGKTILVVEDEETNYKYVEALLNNRLNLVWAKNGADAIKI
                     CVEQDFDMVLMDIKLPKVDGFEATRRIKEFKPKMPIVAVTAYAMEADKNESIQAGCDN
                     YISKPFKLEELFSLINSYLGGQA"
     gene            3834..>4324
                     /locus_tag="PK252_14570"
     CDS             3834..>4324
                     /locus_tag="PK252_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT15979.1"
                     /translation="MDIKEKHILYLDDEKENLDGFKFVFRKEYNIHLASSVKEAFEIL
                     RTQPIKLVLSDNRMPDMLGIEFFEIIAATYPDIYRVLVTAFADTEAALQSINKGKVYY
                     FITKPWNKNELKAVIDNALEAYALKAENNRLIQNLSAKNAELEDLNFKLIVEIAERRK
                     VEE"
BASE COUNT         1541 a          865 c          835 g         1083 t
ORIGIN      
        1 gaatttgcca ttgaagcagg agaaataaat atctgggaca ttgaccttgt tggccacaat
       61 cagttgtaca gccaaagcca cggaaaagtt ttcgggcgac attctgaata tgttactatt
      121 ggagagaaaa tgtgggaact catggtccat cctgaagacc tgccagaggt gaaaaagaaa
      181 tttgcagaac atttcagaaa acaaacaaaa tattttgaaa ccgaatgccg catccaaaat
      241 aaagacaata cttacaaatg gatgctggta agagggaaga ttgcctcaat ggataaaaac
      301 aacatgccac aaaaatacat cggtatgttt atcgatatta cttcgcgtaa gcaaactgaa
      361 gagaaacttg ccgtttcgga agaaaagttc cgtaccatcg tgcaacagtt gtctgatatt
      421 atattcatta ttgatgaaaa tttgactgtc ttgtacgaat ccccgtctgt ttcaaaaaca
      481 tttggatatg aacctggcta ctttttgggg aaaaacgggc tttcgcttat acaccgcgat
      541 gaaataaata tagctaagaa agaatttgag caccttctgc agcataatac cgattttaaa
      601 cccactgaac ttcggatttc ccataaagat gggtattggg tgactttaga aattttgggc
      661 gacaacttga cttctcatcc ggctattaaa ggcatcttgc tcacagcccg cgacatttcc
      721 gaaagaaaag aaaacgaaaa gcagctcgaa ctctatagca accacctcga aaagctagta
      781 aaaaaacgta ccgaagaaat tgaaaacatc aatgccgagc ttatagccat caacgaagag
      841 cttaaagcag ccaatgaaga actatattat aaaaatgaaa agctgaacga agaaatcaag
      901 attaggattg aaacccagtt gatgctcgaa gaaagtgaaa ataaattccg cagctttatc
      961 gaacaatcta ccgaagggat tgtcctcatt gacgaacaag gccgtattgt ggactggaac
     1021 aagggcatgc aatccatttt caggattgcc aaagaagatg ttatcaatac tttcatctgg
     1081 gaatttgact acaaattcct gcccgacaag cgtaaaacgg cacaacagtt tgaagaactc
     1141 aaaaactcta ccatagatta cttctccaac ctggataagt caaaggtaat gaccgttgaa
     1201 ggcatttatc agagcatcga gctcaaacaa aaatacctca acgtaactat tttcccggtt
     1261 atcaccccaa aacgcaggta tcttggccgc atcacacgtg atgtaaccta tattcaccgt
     1321 gcacaggaag agatagaaaa acaaagtgag gaactgcttc aaataaacca gaaccttgag
     1381 gaacaaaaag tacaactcga aaaagcctta gtagaactcc gaaagactca aggccagctt
     1441 atccattccg aaaagatggc ttcactgggg gtattgactg caggtgttgc ccacgaaata
     1501 aacaacccgg taaactatat caatgcagcc cttgaaggac tgaagataac cttacatgac
     1561 ctgtcacaga tttttgaaca atatgattcg ttatcagtcg aaaactcagc gaacaaactc
     1621 aaagaaatag accagctaaa aaccgagctg gattacccca tgttgcagga tggcgtaaaa
     1681 atattgctca acaacatgca aaccggtatt gcccgaatta ctgaaattgt acgaagctta
     1741 cgcacctttg cccgcattga cgaaaacgag ctgcgttaca gcaatatcca cgaattgata
     1801 gacactaccc tgattatgtt gcacaaccaa tacaagaacc gtattgaaat tgtcaaaaat
     1861 tactgcaact ttcaaaacct caactgctac tcaggaaaac tgagccaggt gtttatgaac
     1921 attctgtcaa atgctattca ggcaataccc gaaaaaggga ctattttcat caccaccaat
     1981 gtaaacaaag acaaagaaga tttctccatc tctattcgcg ataacggaat agggatgcca
     2041 gcatatttga aggaaaaaat atttgaacca ttttttacca ccaaagaagc aggcaaaggt
     2101 accggattgg ggttatctat cacttatggg atagtgcaac aacatcaggg gcacattgag
     2161 gtaaactcca aacaaggaca aggaacagaa tttatagtta ccatacccac aaatctaaaa
     2221 taacaagcca tggcaaatag caacaaagaa ccgattctgt atgtagatga tgaatccgaa
     2281 aacttgcaag gttttgaata tcttcttagg cgcgattttg aagtatatgt ggcttcgtct
     2341 gccaaagaag gcttggaaat ccttcagaat acagaaataa aagtacttct gactgaccag
     2401 cgtatgcccg aaatgaccgg tattgaattt ctggaacaag ctataaaaat tgccccggaa
     2461 actatacgta tcgtagtgac agcttatagc gattcggata ccgtcatgca gtctatcaac
     2521 cagggtaaag cctatcactt tatcacaaag ccatggaaca atgcagagct taaaaatgtt
     2581 atcaggcgtg ctgtcgaaac cttcaactta cgacgtgata agatagagct gattacccac
     2641 ctgcaaaatg tcaaccagga gctgttccag gcaaaacaaa gggctgagga ggccgatagg
     2701 ctcaaatcgt cctttctggc caatatgagc cacgaaatac gcacaccgct caatgctatt
     2761 gtcggctttt ccaacctgtt ggtgaacgaa ttgccagcag aggacgagat gcacagacaa
     2821 ttccagcaaa tcatcgaatc cagctcaaat gaccttctca acatcattga ggacatcctg
     2881 gatacatcgc agatagaatc agggtttgtc accataaggc ctactgtaat tgagattaat
     2941 caattcatgg ttgattcatt ggtagcattc caaaaccatc cccaggtaaa agaaaaaaaa
     3001 ctggagatac gttataacta cccaaaaata gaagaaaagc ttacctattt tgttgatgct
     3061 atacgtttaa aacaggtact taacaatatt atgggcaatg ccattaagtt tactgaaaaa
     3121 ggatatattg aaataggcta taaatttacc aatgaagaaa aaaattacat ccaattttat
     3181 ataaaagaca gtggcattgg catccccaat gataagtatg aatatatttt tgaaaggttc
     3241 agaaaaattg aatcttcaac agcagacacc ctttaccgcg gcaacgggtt aggcctttat
     3301 atctcaaaaa ggcttgcaca gatgatggga ggcgatataa ttgttgaatc tgaattggac
     3361 aaaggctcaa cttttacaat tcatctgcct ttttacacca aatcgcctga agagctaaag
     3421 acgataagcc atagccttaa tatatctaaa attgcctggc ctggcaaaac catacttgtt
     3481 gttgaagatg aagaaaccaa ttacaaatat gttgaagcac ttttaaataa tagattaaat
     3541 ttggtgtggg caaaaaatgg ggctgatgcc attaaaatat gtgtcgaaca agattttgac
     3601 atggtgctga tggacataaa actccctaaa gtagatggct ttgaagccac tcgcaggatt
     3661 aaggagttta agcccaagat gcccattgta gctgttacag catacgctat ggaagctgat
     3721 aaaaacgaaa gcatccaggc agggtgcgac aattatattt caaaaccatt caaactcgaa
     3781 gaactatttt cattgattaa ttcatatctt ggcgggcagg cataatgaaa gcattggaca
     3841 ttaaagaaaa acacattcta tatcttgatg atgaaaaaga aaacctggat ggatttaaat
     3901 ttgtctttcg caaagaatac aatatacatc ttgcttcatc cgtaaaagaa gcttttgaga
     3961 ttcttcgcac ccaaccaatc aagcttgtcc tttccgacaa ccgtatgcct gatatgttgg
     4021 ggattgaatt ttttgaaatt attgctgcta catatccaga tatatacagg gtattagtaa
     4081 ctgcatttgc cgataccgaa gctgctttgc agtctatcaa taaaggcaaa gtatattatt
     4141 ttattactaa accctggaat aaaaacgagc tcaaagctgt aattgacaat gctctggaag
     4201 cttatgccct gaaagctgaa aacaacaggc ttattcagaa cttgtctgca aaaaacgctg
     4261 aattagaaga cctaaacttt aaattgattg tcgaaatcgc cgaaagacgc aaagtagaag
     4321 aaga
//
LOCUS       DAOSPF010000132         4071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_22111_length_4071_cov_6.213894, whole genome shotgun sequence.
ACCESSION   DAOSPF010000132 DAOSPF010000000
VERSION     DAOSPF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22111_length_4071_cov_6.213894"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(628..2622)
                     /locus_tag="PK252_14575"
     CDS             complement(628..2622)
                     /locus_tag="PK252_14575"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT15980.1"
                     /translation="MKRTLFIITLLSAFTLYGQIADPVKWTFSHKVINENEVNLVFTA
                     NIDNGYHMYGAYFKEGGPIRTGFTFNPSADYQLKGKIVETTKPTIKKDPFFDNMEISI
                     HAKKAVFTQKVRIEKSNTTIKGKLEYMACTDESCLPPVEKEFAFDIKMPEKIASQKVD
                     TIVKPDTLQIIASEKVDSTAINTPLKSAEPLKSESSLWVFFLVALLAGFAGTITPCVF
                     PMIPMTVSYFMQGANNKGVWRGILFGFSIMLVYTLVGVVVSLTSAGADFANQLSTHWL
                     ANSIFFLLFIVFALSFLGMFEIVLPSGLVNKADRQADKGGWLGIVFLAVATVLISFSC
                     TGPIVGALLVEAASGIAIKPVLGMFAFGLAFAIPFTFFAIFPSAMKKLPKSGGWLNEV
                     KVVMGILMLAFSFKFLVNIDQVYHLNMLSREVVIAIWFVLAMVLGFYLLGKIRTTHDS
                     DVAKVGTLRLILAIALFTLATFLFTGFLGNNMKLFESFFPPKTENISSVQKTIPGNTL
                     CETPKYADLFHLPYGLQGYFELEQAKACAKKLNKPILIDFKGHACSNCKKMDAEVWSV
                     PEVQQYIADNFVIAALYTDDRTELTKEDQVTSKIDGKVKLTIGQKNANIQAEMFNSNA
                     LPLYVIISPEGQPLVSPVGSIFDAKKYLEFLKNGVSMYNK"
     gene            2907..4019
                     /gene="gcvT"
                     /locus_tag="PK252_14580"
     CDS             2907..4019
                     /gene="gcvT"
                     /locus_tag="PK252_14580"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT15981.1"
                     /translation="MTDQPVKTTPFVNVHKALGAKMVPFAGFLMPVEYSGINVEHQTV
                     RENVGIFDVSHMGEIWVKGDKALDLVQWVTSNDASKLKPGQIQYSCFPNGKGGIVDDL
                     LVYMYDSKKFLLVVNASNIDKDYNWLVSQNKFGVEIENSSDNIGQLAIQGPKATELLQ
                     NLTSVDLKTIPYYTFVTGNFAGCNDVIISNTGYTGSGGFEIYFYNRDAKKVWDAIFAI
                     GEKYGLKPIGLGARDTLRLEMGYNLYGNDIDDTTSPIEAGLGWITKFTDSKDFVDKEL
                     LLKQKNVGISKKLVGFEMVDKAIPRHGYNITDESGKAIGIVTSGTMSPTMKIGIGMGY
                     VPTALAEVGSTVYVEIRGKLQKAKVVKMPFYKGGIK"
BASE COUNT         1313 a          720 c          790 g         1248 t
ORIGIN      
        1 gcattatctg gttactttta tgagtgttta cacaatcttt tttatactac caaataaaaa
       61 ctaaactgca gaggtcacag agtttttcgc aaagaaccgc aaagaataat tttaaatctc
      121 tgttgcgcct taatgtcatt tatttataac tccgaaggag tgttctgttt atagataata
      181 tgtgatatat aattaatagc gcgccagaag gatgatagca aaagtcagtt tcttatttgg
      241 cgtgcaataa aaggacttcg tattaacagg ttgcgtattg tgcacctata gttgatgtac
      301 atttatcaaa tcatctgcat ggtgcgcttt tgctgtaaag aaatttttct ataaacatta
      361 agctcctatg gagctttgaa aatattaaga attactgttg tccggtaaaa taaaatagat
      421 acttataaat actatagtaa tatcgttgat tagaaacagg tcaccccgtt ggggcttaat
      481 tttaacgatg tttaatttta ctacaaacag gacatcgcta tgcgactaaa taatagcagc
      541 gtagctgcgt aggataaccc aatacaccaa atgattttat gccgcacagc ggcgacctgt
      601 ttttaccgga caacaatgat taagaatcta tttattatac atagacaccc catttttcaa
      661 gaattctaaa tactttttag catcaaatat actaccaacg ggagaaacca acggttgtcc
      721 ttctggcgag ataatgacat aaaggggtag ggcatttgaa ttgaacattt ctgcttgtat
      781 attggcgttt ttctgaccaa tagtaagctt aaccttacca tctatcttgg atgtaacctg
      841 gtcttctttg gttaattcag tcctgtcatc ggtgtagagg gcagcaatta cgaagttgtc
      901 agcaatatat tgctgaactt caggtacaga ccagacctca gcatccatct ttttacaatt
      961 gctgcatgca tgtcctttga agtcaataag gataggtttg ttgagctttt tagcacaagc
     1021 tttggcctgt tccagctcaa aatagccttg aagtccatag ggtaaatgaa acaaatcagc
     1081 atatttaggt gtttcgcaca aggtatttcc cggaatggtt ttttgaacag agcttatgtt
     1141 ttctgtcttt ggcggaaaga acgattcgaa caatttcata ttattgccca agaagccggt
     1201 aaataagaat gtagcaaggg taaataatgc tatagccaaa attaatcgta atgttccaac
     1261 ttttgcaaca tctgagtcat gcgttgtacg tatttttcct aacagataaa atcccagaac
     1321 catagccaat acaaaccaaa tagcaataac cacttcgcgt gacagcatat tcaggtgata
     1381 tacctgatcg atatttacca ggaatttaaa gctaaaggcc aacataagga tacccatgac
     1441 aaccttaact tcattgagcc atccgccaga cttaggcagt tttttcatgg cagaagggaa
     1501 gatggcaaag aaagtaaaag gaatggcaaa agctaagcca aaagcaaaca tgcccagtac
     1561 aggtttaata gctataccgc tggctgcttc gaccagtaat gccccaacaa taggcccggt
     1621 gcaggaaaac gatattagca ctgtggcaac ggccaaaaat actatgccta accatcctcc
     1681 tttgtcagct tgtcggtctg ctttgtttac caggcctgat ggtaagacta tctcaaacat
     1741 tcctaaaaat gacaaggcaa atacaataaa aagcagaaag aatattgaat tggcaagcca
     1801 atgtgtgctt agttggttgg caaagtctgc tccggcactg gtcagcgaaa ctacaacgcc
     1861 taccagcgta taaaccagca tgatgctgaa cccgaaaagt attcctctcc agacaccttt
     1921 attattagct ccttgcataa aatatgacac tgtcattggg atcatcggaa acacgcatgg
     1981 cgtaatagtg cctgcaaaac ctgctaacaa agctactaaa aaaaataccc ataatgaaga
     2041 ctctgacttt aggggttctg ctgattttaa gggtgtattt attgcagttg aatcaacttt
     2101 ttctgatgca ataatttgta aagtatctgg ttttactata gtatccactt tttgagatgc
     2161 tatcttttca ggcattttaa tgtcaaaagc aaattccttt tcaactggcg gtaaacagga
     2221 ttcatccgta caagccatgt attcgagttt accttttata gtggtattgc ttttttcaat
     2281 tcgtactttt tgggtaaata cagctttctt agcatgaata ctgatttcca tattatcaaa
     2341 aaatgggtct ttcttgatgg ttggcttggt tgtttcaaca attttgcctt ttagctgata
     2401 gtcagcagac ggattaaatg taaaacccgt ccttatagga cctccttcct taaaataagc
     2461 cccatacatg tggtagccat tatcaatatt ggctgtaaac acaaggttta cttcattttc
     2521 attaataact ttatgtgaaa atgtccattt tacagggtcg gcaatttgac catacaacgt
     2581 aaaagccgaa agtaaagtga tgataaaaag tgttcttttc ataatcgtaa gtttgataat
     2641 gtaaaattaa tcaagttaag caaacaagca attagcacta ttatagaaat atctacttct
     2701 tttatgtttg caacaatgat atttactaat aatatgcaaa tatatatata attatatgtt
     2761 cataaaatct aaaaaatgtt aacaagtgcc caatctcttt tcaattcttg aaaacatcaa
     2821 tttgaactga cattattggg ctttgttgct attaacaaaa tatcagccaa tgacaaaatt
     2881 cataaatttg cataaaaagt tgcactatga cagaccaacc agttaaaact acgccttttg
     2941 ttaatgtgca taaggcttta ggagctaaaa tggttccatt tgcaggattt ttgatgcctg
     3001 tggaatattc cggaattaac gttgaacatc aaaccgttcg cgaaaatgtt gggatatttg
     3061 atgtttcgca catgggcgaa atatgggtta aaggcgacaa ggcattggac ttagttcaat
     3121 gggtgacttc caatgatgct tcaaaattga aacccgggca aatccaatat tcttgtttcc
     3181 caaacggaaa aggaggcata gtagatgatt tgctggttta catgtacgac agcaagaaat
     3241 ttctattggt agtcaatgct tctaatatag acaaggatta caactggctg gtaagtcaga
     3301 ataagtttgg ggtagagatt gagaatagta gcgacaatat tggccagctt gccattcaag
     3361 gcccaaaggc gacagagtta ttgcagaacc tgacctctgt tgatttgaaa actattcctt
     3421 attatacttt tgtaacaggc aattttgccg gatgtaatga tgttatcatt tcaaatactg
     3481 gctataccgg ctctggtgga tttgagatat atttttataa ccgtgatgcc aagaaagtct
     3541 gggatgccat ttttgcaata ggcgaaaaat atggattgaa acccataggc cttggagccc
     3601 gtgatacctt acgtttggaa atgggatata acctgtatgg caatgatatt gatgatacca
     3661 cttcgcctat tgaagcgggt ttgggttgga taaccaaatt tactgattcg aaagatttcg
     3721 tggacaaaga gttgttgttg aaacagaaaa acgtcggaat ttcaaagaag ttagttggtt
     3781 ttgaaatggt ggacaaagct attccgcgtc atggatataa tattaccgat gaaagtggca
     3841 aagccatagg cattgtaaca agtggtacta tgtctcctac tatgaagatt ggtattggca
     3901 tgggttatgt tcctacagcc cttgcagaag ttggctcaac ggtttatgtt gaaattaggg
     3961 gcaagcttca aaaggctaaa gtggtgaaga tgccatttta taaaggaggg attaaataat
     4021 tagcaaatta gcaaattagc aaattagcaa attagcaaat tagcaaatta g
//
LOCUS       DAOSPF010000133         4008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_22604_length_4008_cov_7.379206, whole genome shotgun sequence.
ACCESSION   DAOSPF010000133 DAOSPF010000000
VERSION     DAOSPF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22604_length_4008_cov_7.379206"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..707)
                     /locus_tag="PK252_14585"
     CDS             complement(312..707)
                     /locus_tag="PK252_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT15982.1"
                     /translation="MYLLDTNICIYTINKKSVEVLSKLKKKVSKDIYISSITVAELEH
                     GVEKSNYPERNKVALVEFLSLFQILYFTDGDAFYYGKIRSSLEKRGQIIGTMDMLIAS
                     QALFHDLILVTNNTKEFERIENIKLENWT"
     gene            complement(707..1015)
                     /gene="vapB"
                     /locus_tag="PK252_14590"
     CDS             complement(707..1015)
                     /gene="vapB"
                     /locus_tag="PK252_14590"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOT15983.1"
                     /translation="MSIKILFYFLIDQIYITIYITFKVLKMQTAKLFINGRSQAVRLP
                     KEYKFSGNEVIIQKVGEAVILLPHNKSWEVFLHGLNSFSDDFMSDGRAEEINQNRESF
                     "
     gene            complement(1093..1821)
                     /locus_tag="PK252_14595"
     CDS             complement(1093..1821)
                     /locus_tag="PK252_14595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOT15984.1"
                     /translation="MEEVKVLHITSNLSIDVVRKDIKNMHLAVYPPTGRVRIATPLNV
                     NDEAVRLFAISKIAWIRKHQRHFKAQDRQSPREFIQRESHYFQGKRFLLRITEHKASV
                     KVEIKTKTYIDLYIKPNSTIEQKQIAINEWYRAELKKLIPEIIQKWEQKIGVQVSEWQ
                     VKQMKTKWGTCNIEKKRIWINLELAKKPIHCLEYIIVHEIIHFIERHHNERFMALMDK
                     YIPQWKFFKEELNRLPVSHGEWDY"
     gene            complement(1824..>4008)
                     /locus_tag="PK252_14600"
     CDS             complement(1824..>4008)
                     /locus_tag="PK252_14600"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOT15985.1"
                     /translation="EYNPNARVLIVTDRDELDKQIEKVYKGVSEDIYRCKPRNGKSGG
                     AILIDKLNDTEPWLLCSLIHKFGNKEEADYDSYLTELKNSLPKDFSPKGDFYVFVDEC
                     HRSQSGDLHKAMKQLLDERAIFIGFTGTPLMKKDKQKSIEVFGKYIHTYKFDEAVKDG
                     VVLDLRYEARDIEQKISSLDKIDTWFETKTKGLTDFAKIELKQKWGTLKKVFSSVGRL
                     QKIVADILLDMETRERLQNGRGNALLVSDSIYNACRYYQLFQESGFTRCAIVTSFAPS
                     HADIKGEGENYTEKIMQYEIYQKMLNGKSPEDFEDEVKKRFVDEPAQMKLLIVVDKLL
                     TGFDAPSATYLYIDKSMRDHGLFQAICRVNRLDGDDKEYGYIIDYKDLFGSLETAYND
                     FTSKAFEDYDKEDVEGLLSDRLNKGKERLNDALETIRALCEPVVPPKGTKEYIQYFCG
                     NTQIEADLKDSEPRRHALYKAVISLIRAFANIADELVEAGYKPNEIEKIKAEIKHYEN
                     VRKEIQLASGDYVDLKQYEPAMRHLIDSYIGAEESQKISAFDDFSLVELLVKDGEDAL
                     DKIPENIRKNKDAMAETIENNMRKVIIEESPTNPIYYEKMSVLLDELIKMRNEEADRY
                     EEYLKKIVELAVKVKKPETSSEYPKSINTQAKRALYDNLDKHEPLTLVMDSAVKYGKY
                     DNWEGNFAKERHLKNKVVKPVLEDYNKVEVLDPIFEIIKQQNEYK"
BASE COUNT         1098 a          806 c          676 g         1428 t
ORIGIN      
        1 ttattttttt agaaagcttt taatccttac ggttcacaac gcttccctta acttctaata
       61 tctcaatatt aaaatccgca aatactcctg cagtttttca cctaatgcct ttttccgact
      121 ctgtgtgctc cggttaaagc tattatcaat tatattatcc tgtttaaaat accgtttagc
      181 attccgtaaa caatgaacat acgccgaaac agaaatgcgg caaaacacaa acaccgcgcg
      241 gaacgaagta agcacggagc tgttgcacga aggagcgcgc agccgaagtt atcggcagct
      301 gcacatatct attaagtcca gttttcgagt ttgatatttt caattctttc aaattctttt
      361 gtattatttg taacaagaat taaatcgtga aataatgcct gtgaagcaat aagcatatcc
      421 atcgttccaa ttatttgccc tctcttttca aggctggatc tgattttgcc gtaataaaac
      481 gcatctccat cggtgaaata gagtatttgg aataaagata agaattcaac aagagctact
      541 ttgttacgct ccggataatt acttttttct acaccgtgtt caagttcggc aacagttata
      601 gatgaaatat aaatatcttt tgacactttt ttctttaatt tggatagaac ttcaacggac
      661 tttttgttga tggtgtagat acagatattt gtatccagca aatacatcag aatgattctc
      721 tattttgatt gatctcttcc gcccggccat cggacatgaa gtcatcagaa aagctgttta
      781 aaccatgaag aaacacttcc catgatttgt tgtgagggag cagaataaca gcctcgccga
      841 ctttttgaat aatgacctcg ttgccggaga atttatattc ttttggcaat ctgacggcct
      901 gacttcttcc attaataaat agtttcgcag tttgcatttt caacaccttg aaagtaatat
      961 atatcgtgat atatatttgg tcaatcaaaa aatagaacaa gattttaatc gacaaatagg
     1021 cttgtttaat tggcgatgat gagtcgcaag taaatgacgt cgccggagcg cgcggaggag
     1081 ttcatcctgt ttctaataat cccattcacc atgacttact ggtaatctat ttagttcttc
     1141 tttgaaaaat ttccactgag gaatatattt atccatcaaa gccataaatc tctcgttgtg
     1201 atgcctttca atgaaatgaa taatttcgtg aacgatgatg tattccaaac aatgaatcgg
     1261 tttttttgct aattccaggt ttatccaaat tctttttttc tcaatgttgc atgttcccca
     1321 tttggttttc atttgcttta cttgccattc ggaaacctgc acaccaattt tctgttccca
     1381 cttttgaata atttctggaa taagcttttt taactctgca cgataccatt cattgatagc
     1441 aatttgcttt tgttcaatgg tactgtttgg tttgatatat aaatcaatat acgtttttgt
     1501 ttttatctca actttgacgg aagctttatg ttctgttatt ctcaataaaa accgtttgcc
     1561 ttgaaaataa tgactttccc gttgtataaa ttctctgggc gattgtctgt cctgtgcttt
     1621 aaaatgtctt tgatgtttac gaatccacgc aatttttgaa atagcaaaaa gtcgtacagc
     1681 ttcatcgttt acatttaaag gagttgcaat acgaaccctg ccagttggcg gataaacagc
     1741 caaatgcata ttcttgatgt cttttcgaac tacatcaatg gatagatttg aagtaatatg
     1801 taaaactttg acttcttcca tttctatttg tactcgtttt gctgtttgat aatctcaaaa
     1861 atagggtcta aaacttcgac tttgttgtaa tcttctaaaa caggttttac cactttattt
     1921 tttagatgtc tttctttggc aaaatttcct tcccaattat catatttacc atatttgaca
     1981 gcactatcca ttactagtgt taaaggctca tgtttgtcta aattatcata taaagccctt
     2041 tttgcctgag tgtttatgct ttttggatat tcgctgcttg tttcaggttt tttaactttt
     2101 acagccagtt cgacaatttt ctttaaatat tcttcatagc ggtcagcttc ttcattacgc
     2161 atttttatca gttcatcgag caagacactc atcttctcat aataaattgg gttggttgga
     2221 ctttcttcaa taatcacttt tcgcatatta ttctcgatgg tttctgccat tgcatcctta
     2281 tttttcctga tattctcagg aattttatcc aatgcatctt caccatcttt aacaagcagt
     2341 tcaaccagac taaaatcgtc aaaagccgaa atcttttggc tttcctcagc accaatgtag
     2401 ctgtcaatta aatgacgcat tgccggttcg tattgtttta aatccacata gtcgccactt
     2461 gccaattgta tttctttacg aacattctcg taatgtttga tttccgcttt tatcttctca
     2521 atctcattgg gtttataacc tgcttctacc aattcgtctg caatgttggc aaatgcacga
     2581 attagtgaga ttaccgcttt gtataaagcg tgtctgcgag gttccgagtc ttttaagtca
     2641 gcttcaattt gagtattacc acagaaatac tggatgtatt ctttcgtgcc ttttggcggt
     2701 acaaccggtt cacataaagc ccgaatggtt tccaacgcat catttaggcg ttcttttcct
     2761 ttgttcaaac ggtcggatag caaaccttcc acatcttctt tgtcgtaatc ttcaaaggct
     2821 tttgaggtga agtcattata tgctgtttcg aggcttccaa aaaggtcttt gtagtcaatt
     2881 atataaccat attctttatc gtcaccatct aaacggttta cacgacaaat tgcctgaaat
     2941 aaaccatggt ctcgcatact tttgtcgata tacaaatagg ttgcacttgg agcatcaaaa
     3001 cctgtaagca gtttatctac aacaatcaaa agtttcattt gtgcaggttc gtcaacgaag
     3061 cgttttttta cttcatcttc aaaatcttcg ggtgatttac cattgagcat cttttggtaa
     3121 atctcgtatt gcatgatttt ctcggtgtag ttttcgcctt cacctttaat gtcagcatga
     3181 gatggggcga atgatgttac aatggcacaa cgggtaaagc ccgattcctg aaacaattgg
     3241 taataacgac aagcattgta aatactgtcc gaaactaaaa gtgcatttcc cctgccattt
     3301 tgtaaacgct cacgggtttc catatcgagc agaatatcgg caacaatctt ttgtaaacgc
     3361 ccaactgagc tgaatacctt tttcagggtt ccccattttt gttttagttc tatttttgca
     3421 aagtcggtta ggcctttggt ttttgtttca aaccatgtat caattttatc cagcgagctg
     3481 attttctgtt caatatctcg ggcttcgtaa cgtaaatcaa gaacaacacc gtccttaacc
     3541 gcttcatcga atttataggt atggatgtat ttgccaaaaa cctcaatact tttttgctta
     3601 tcttttttca ttaaaggcgt gcctgtaaaa ccgatgaaaa tagctctctc atccaataac
     3661 tgtttcatag ctttatgaag gtcgccactc tgagaacggt gacactcatc tacaaacaca
     3721 taaaaatccc ctttgggtga aaaatccttc ggcagactgt ttttcagttc ggtcagataa
     3781 ctatcgtaat cggcttcctc tttgttgcca aatttatgaa tgagtgaaca caaaagccag
     3841 ggttcggtgt cgttgagttt atcaatgagt attgcaccac cactttttcc gttacggggt
     3901 ttgcatctgt aaatatcttc gcttacgcct ttgtaaactt tttcaatttg tttgtcgagt
     3961 tcgtcacggt cggttacaat taaaacacgg gcattggggt tgtattct
//
LOCUS       DAOSPF010000134         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_23460_length_3890_cov_5.551239, whole genome shotgun sequence.
ACCESSION   DAOSPF010000134 DAOSPF010000000
VERSION     DAOSPF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23460_length_3890_cov_5.551239"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..1758
                     /locus_tag="PK252_14605"
     CDS             493..1758
                     /locus_tag="PK252_14605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT15986.1"
                     /translation="MNVKIIEETSPLDDEVPLQLVGYNKISENVKSNLFRLKKTEFST
                     ISPQTKNFIQKHFPNTPEEQWNSWRWQIKNSFTTFEQLSEVVDVKQLMNEAFIAAHKK
                     LPLRITPYYASLLEGADINNPIFKGVVPSIKELQMSPGEASDPLHEEKCSPVPNIVHR
                     YPDRVLFLATGFCSTYCRYCTRSHMVSKEKVHYGKAAWQEGIEYVRNNKNVRDVLISG
                     GDPLTMPEAHLEYLLESLRNISHVEIIRIGTKVPVVLPQRITNSFVNMLRRYHPLFMS
                     IHFMHPMELTPEVEEACSKLANAGIPLGSQTVLLKGINDSVTVMTKLMQGLLKNRVRP
                     YYIYQCDPIPGSGHFRTSVEKGLEIIKGLRGFTSGYAVPHFVIDAPGGGGKIPLLPEY
                     YIGRDENNVILLNYEGKKYYYPDAPVNYK"
     gene            1787..2773
                     /locus_tag="PK252_14610"
     CDS             1787..2773
                     /locus_tag="PK252_14610"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT15987.1"
                     /translation="MKIGLTYDLRSDYLKEGYSMEETAEFDKEETIIGIEEALKCNGY
                     QTERIGHARKLMQELLKGKRWDMVFNISEGMYGDSRESLVPALLDAYQIPHVFSGAVT
                     LGISLNKAFAKQIVRDAGVNTPPFMVVYQPDDVKHSTLTYPLFAKPIAEGTGKGIEGR
                     SVINTPEQLAEICQYLLKKFNQPVLVEEYLPGREFTVGVVGNGTDARVVGAMELIYNE
                     GIDNVYSYFNKENYEGRIHYQPVTGEWLAQCADVTLKAWNALKAFDGGRVDVRVDRHG
                     KVSFIEINPLAGLNPVHSDLPILCRMNNIEYNQLIGMIMEAAIKRIQSNHGK"
     gene            2763..3734
                     /locus_tag="PK252_14615"
     CDS             2763..3734
                     /locus_tag="PK252_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT15988.1"
                     /translation="MGNKKVIILHNAVEADSKPDEADVLHQVELVSAALTELGYLPEA
                     FPLGVDLKEGMEKVLSKSPLFVFNLVESVFDKGELLYIGPALLNAFKIPYTGVSVDSL
                     FITTNKVLTKRVLQQNNIPTPQYFSTSETNLLKPNTRYLLKPTSEDGSVGLDEDAVFT
                     TEQPAMIEKIKQLPSSHYFIEEFIEGREFNISVLGGKDKVDVLPPAEMLFVDFPESKP
                     QMLGYTAKWNEDSVEYKNTTRAFDTLSPESALYKELIRLTRQCWDCFSLNGFARVDFR
                     VDENEKPYVIEINGNPCISPDSGFIAAARIVGFSDKEVVQRIVEDLN"
BASE COUNT         1222 a          743 c          788 g         1137 t
ORIGIN      
        1 ttcgcaaagg aagggggaga cgtttttcca ttaacaacgt ggtgaaacta aacagttatt
       61 tgctatatcg gccatgtttt tttgctaagg caataaattt tgttgaatat tcattaattt
      121 ttatttgtcc tgaaagttta tagagttgct ttttttgtgc aattctgtga ttttttgatg
      181 tttaatcttg tgttaaatta gttaatcttt ttgataaatc aaagagagca aatgataaat
      241 aataagaatt gtttttatct ccgaatggca ttaaaattgt taataacctc tcttttttaa
      301 ttattgaata taatagaaat aggttattat aagagcttaa ggccggttaa taaataattg
      361 taaaatttta tacccaagta ttacaaccaa tgtataaatg aagtaatatt cggagaaaaa
      421 caaaactaaa acgaatggat ataaaacttt gatattcaat gttaaataaa agtcaggata
      481 aaaattaaaa caatgaatgt aaaaattatt gaggaaactt ctcctcttga cgatgaggta
      541 ccgctacaac ttgtgggcta caacaaaatc tccgaaaatg ttaaatcaaa ccttttcagg
      601 ttaaagaaaa ctgaattttc taccattagc ccacaaacta aaaactttat acaaaagcat
      661 tttcctaata cccccgaaga acaatggaac tcctggagat ggcaaattaa aaacagtttc
      721 actactttcg agcagttaag tgaagttgtt gacgtaaagc aactgatgaa tgaagctttt
      781 attgccgcgc acaaaaaact tcccctaaga ataacccctt attatgcaag tcttttagaa
      841 ggagcagata tcaacaatcc aatatttaaa ggcgttgtcc ccagcatcaa agagttgcaa
      901 atgtcaccag gcgaggcttc tgaccctttg catgaagaaa agtgttcgcc tgttccaaat
      961 atcgtacacc gataccccga cagggtgttg ttcctggcca caggtttttg ttctacctat
     1021 tgcaggtatt gcaccaggtc gcacatggtg tcaaaagaaa aggtacacta tggcaaagct
     1081 gcctggcaag aaggtatcga atatgtccga aataataaaa atgtccgcga cgtgcttatt
     1141 tcgggaggcg atccgttaac catgcctgaa gctcacctcg aatacttgct ggaatcgtta
     1201 cgtaatatct ctcatgtaga gataatccgc attggcacaa aggttccggt agtactgcca
     1261 caacgtatta ccaacagctt tgtaaatatg ttgcgcagat accatccgct gttcatgagc
     1321 atacacttta tgcaccctat ggaacttacc cctgaggtgg aagaggcttg ttcaaaactt
     1381 gctaatgccg gaatcccact tggcagccag acagtattat tgaaaggtat caatgactct
     1441 gtaaccgtta tgaccaagct aatgcaaggt ttattaaaga atagggtgcg accttattac
     1501 atttatcaat gcgacccgat acccggctca ggccattttc gcacctcggt cgaaaaaggt
     1561 ttagaaatca tcaaggggtt gcgcggcttt acaagtggtt atgctgtacc ccactttgtt
     1621 attgatgccc ctggtggagg agggaaaata ccacttttac cagaatacta tattggccgt
     1681 gatgaaaata atgttatcct tctgaattat gaaggaaaaa aatattatta ccctgatgct
     1741 cctgttaatt ataaataaaa tggttcattt caataaatgt caataaatga aaataggact
     1801 aacctatgac cttcgttctg actacctgaa agaaggctac tctatggaag aaactgccga
     1861 gtttgacaaa gaagaaacta ttattggtat tgaagaagcc ttaaaatgca atggttacca
     1921 aacagaacgt attggccatg cccggaagct gatgcaggag ttattgaaag gaaaacgatg
     1981 ggatatggtt tttaacatca gcgaaggtat gtatggcgac agccgcgaat ctttagtgcc
     2041 ggcactgtta gatgcatacc agataccgca tgttttttca ggtgcggtta cgcttgggat
     2101 atccttaaac aaagcttttg ccaaacagat tgtacgtgat gccggcgtaa acactccgcc
     2161 atttatggtg gtttatcaac cggatgatgt taaacactct acactaacat atcctttatt
     2221 tgccaaacct attgcagaag gtacaggaaa aggtattgaa ggccgctcag tgataaatac
     2281 cccggaacag ctggccgaga tatgtcaata ccttctgaaa aagtttaatc aaccagttct
     2341 ggttgaggaa tacctgcctg gccgcgaatt tactgtaggt gtagtaggta atggcactga
     2401 tgcccgcgta gttggtgcta tggaacttat ttataatgaa gggatagata acgtttattc
     2461 ttattttaat aaggaaaact acgaaggccg catacattac caacctgtaa caggcgaatg
     2521 gcttgcccaa tgtgccgatg taacgctgaa agcctggaat gcccttaaag ccttcgatgg
     2581 aggtcgtgtt gatgttcgtg tggacaggca tggaaaggtg agcttcatag aaatcaaccc
     2641 ccttgccgga ttgaaccctg tacattctga cttgcccatc ctttgccgga tgaacaacat
     2701 tgagtacaat cagctcattg gtatgattat ggaagcggcc atcaaacgca ttcaatcaaa
     2761 tcatgggaaa taagaaggtt attatcctgc acaatgctgt cgaggcagat agcaagccgg
     2821 acgaagctga tgtgcttcac caggtcgaac tggtttcggc agctttgaca gaattaggtt
     2881 atctacccga agcttttcct ttaggagtcg accttaaaga agggatggaa aaagtccttt
     2941 caaaatcacc gctgtttgtt ttcaatttgg tagaatctgt ttttgacaaa ggagaactgc
     3001 tgtacattgg gccagcattg ttgaatgctt ttaaaattcc ttatactggt gtttctgtcg
     3061 atagtttgtt tattactacc aacaaagtgc ttaccaaaag ggtattacaa caaaataata
     3121 ttcctactcc gcaatatttc tcaacttcag aaaccaacct tctaaaaccc aacacccgct
     3181 atttgttgaa accaaccagt gaagatggct cagtaggttt ggacgaagat gctgttttta
     3241 ccacagaaca gcctgctatg attgaaaaaa taaagcagtt gccctcttct cactatttta
     3301 ttgaggaatt cattgaaggt cgcgagttca acattagtgt tttaggaggg aaagacaagg
     3361 ttgacgtgtt gccacctgcc gaaatgcttt ttgtggattt cccggaaagt aagccgcaaa
     3421 tgcttggcta tactgccaag tggaatgaag attctgtgga atacaagaac actacaaggg
     3481 catttgatac cttgtctcct gaatctgcat tgtataaaga attgatacgc ctgacacgtc
     3541 aatgctggga ttgctttagc ctgaatggct ttgcccgcgt tgatttcagg gttgatgaaa
     3601 atgaaaagcc ttatgtcatt gaaatcaacg gcaacccttg catttcgccc gatagcggct
     3661 ttattgccgc tgcccgcatt gtcggctttt ctgacaaaga ggttgttcaa agaattgtag
     3721 aagatttaaa ctaatgtgat ttcgatataa taatttcatt gtcaattatt atatgaaaaa
     3781 tattgaataa caataaatac aaagtctatg ctactttttt atcgaaaaaa gtagccaaaa
     3841 actcttatcg ccttaactct ttcaccctcg aagcctctta atgttgcaat
//
LOCUS       DAOSPF010000135         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_25243_length_3673_cov_6.114428, whole genome shotgun sequence.
ACCESSION   DAOSPF010000135 DAOSPF010000000
VERSION     DAOSPF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25243_length_3673_cov_6.114428"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..627)
                     /locus_tag="PK252_14620"
     CDS             complement(187..627)
                     /locus_tag="PK252_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15989.1"
                     /translation="MKKALIPILVIFLVSCNTAEKALNKAEMLEIVKKYDAQFSIGIK
                     NKDSAYLANIYSDSAQYVTPHRPILKGKEEIAKDWGGFLRLKEEPIDIILNIIEVRGN
                     REIIYETGEGYTLLADSTKWPFNYVNVWRLQKDGSYKLEIDTYN"
     gene            721..1320
                     /locus_tag="PK252_14625"
     CDS             721..1320
                     /locus_tag="PK252_14625"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT15990.1"
                     /translation="MLKAFFKEGVIEAGCDEAGRGCLAGPVFAAAVILPQGFFHPLLN
                     DSKKMTEKNRETLRDIIKKEALAFAVATCSAEEIDNINILNASFLAMHRAIDQLKTRP
                     EFLLIDGNRFNPYPNLQHKCIIGGDALYQSISAASVLAKTYRDKYMQSLHNEYPEYNW
                     KKNKGYPTTDHRLAIKKHGLTPHHRHTFAAKFIEPSLFD"
     gene            complement(1449..>2410)
                     /locus_tag="PK252_14630"
     CDS             complement(1449..>2410)
                     /locus_tag="PK252_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HOT15991.1"
                     /translation="LSLIKKAMVAAGYEDIPVISIASGDSLLNPQPGFDVKWRKALTI
                     TAVSMLYADCISKMYYATVVRQKVQGGAVSLRDKYINAALPFAEKKDIRGMLKLLKQA
                     AAEFNSMCTDKEFVPRIGIVGEIYVKYNAFGNKNVVNWLIEQNVEVVMPSLANFFLQY
                     FANYKINVAKHLEKPGLPQWIVDILFALVDRYIKKVENAAKDFKYYRPFTNIYTDAEK
                     AEEIVNTAAQFGEGWLIPAEIANFAEENVNYVVSLQPFGCIANHVVAKGIERKLKHRF
                     PSMNMLFLDFDSGVSDVNILNRLHFIIKGAEQQAEKEAEKATV"
     assembly_gap    2411..2510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2511..>3673)
                     /locus_tag="PK252_14635"
     CDS             complement(<2511..>3673)
                     /locus_tag="PK252_14635"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOT15992.1"
                     /translation="HGHIIDLIDKKANRIFMPYVVYESKEDKNTPNSFNCPIVSGYSD
                     VIKSSMPFLEKHNICIDAPTIAFNNEQLLTKACIKYLMTLGIEKSLVEKALPKALAAQ
                     RMYVSSLQENCQRIIEKNSVGNNMVIMLVGRPYHADPLIQHKISDMISAFGVDVISED
                     FVRSEEHNTFDNVQTIMQWAYTNRILKAAEWVAKTNANVHLVQMTSFGCGPDAFIIDE
                     VNEILKRHGKSHTILKIDDVNNIGSLRLRIRSLIESLRFASDKEVTITPLIKNKIFTE
                     EDKHRTILVPFFSEYYSPFVPVLFKLAGYNFVTLPPSDVHSSELGLKFANNEVCYPAT
                     LVIGDIIKALQTRKYNRDEIAIGITQTGGQCRATNYLSLIKKAMVAAGYEDIPV"
BASE COUNT         1084 a          692 c          661 g         1136 t
ORIGIN      
        1 agcacctgac tcataatcag ggggtccctg gttcaagccc aggtgggccc acccctgaaa
       61 gtcccgagaa atcgggattt tttatatggt aaaaaacaat aggctggtaa gtcaaacgac
      121 aattccagcc tattgttttg tttgtcaaat ccgtagatct gaaaaactga atatatagtt
      181 agtcagctag ttgtaggtgt ctatttctaa tttatacgaa ccatcttttt gcaaacgcca
      241 aacattgaca taattaaatg gccatttggt agaatcggcc aaaagcgtgt acccttcacc
      301 tgtttcgtaa ataatttcac gatttcctcg aacctcaata atatttagaa ttatgtcaat
      361 aggttcctct tttaatctga ggaaaccacc ccagtctttt gcaatttctt cttttccctt
      421 taatattggc cgatgcggtg taacatactg ggcactgtct gaatatatat tagccagata
      481 tgctgagtct ttgtttttaa taccaatact gaattgtgca tcgtatttct tgacaatttc
      541 taacatctct gctttgttta gtgctttttc tgcagtatta catgaaacca aaaatataac
      601 aaggattggg atgagcgctt ttttcatttt cttaagaatt tattatgaat aactactttt
      661 gcactatcac atttatatga tgagttaccc ttattaaata tttttgaaaa aaatttcatg
      721 atgttaaaag ctttttttaa agagggtgtt attgaagctg gctgcgatga agcaggcaga
      781 ggatgccttg cagggcctgt atttgccgca gcagtaatac ttcctcaagg cttttttcat
      841 ccattgctca atgactcgaa aaaaatgact gagaaaaacc gggaaacatt gagggatata
      901 attaaaaaag aggcactggc ttttgctgtt gcaacctgtt ctgcagagga gatagacaat
      961 attaatattt tgaatgcttc ttttttggct atgcacaggg ctatagatca attgaaaaca
     1021 agacctgagt ttttattgat tgatggaaat cgttttaatc cataccctaa ccttcaacat
     1081 aagtgtataa ttggtggaga tgccctatat cagtcaattt ctgcagcatc agtacttgca
     1141 aagacttaca gagataagta tatgcaatca ttgcataatg aatatcccga atataattgg
     1201 aaaaagaaca aaggttatcc aaccacagat catcgtctgg ccataaagaa acatggactt
     1261 acgccacacc acaggcacac ctttgctgca aagtttatag agccttcgtt gtttgattga
     1321 aatgtcgtac aaaataggtc caagtcaaag acttgaacta taggcgatta acattgtatg
     1381 aaaaattagc ccaagtcaaa ataaaagact taaactacca aaaattgcct cattatatgt
     1441 gaggcgaatt ataccgtagc cttttctgct tctttttcgg cctgttgctc agctcctttg
     1501 atgataaaat gcagacggtt caggatgttt acatcactca caccgctatc gaagtctaag
     1561 aaaagcatat tcattgaagg gaaacgatgt tttagttttc tttcgatacc tttagctaca
     1621 acatggttgg caatacatcc aaaaggctgt aaacttacca catagttaac attttcttca
     1681 gcaaaattag caatctctgc aggtatcaac caaccttcac caaattgggc tgcagtattg
     1741 acaatctctt cagccttttc agcatctgta tagatattgg taaatggtct gtaatactta
     1801 aagtctttgg cagcgttttc aacttttttg atatacctgt caaccaaagc aaagagtatg
     1861 tcaacaatcc attgaggcag cccaggtttt tccagatgtt ttgcaacgtt tatcttatag
     1921 ttggcaaaat attgcaggaa aaagttggcc agcgaaggca taaccacctc aacattttgt
     1981 tcaataagcc agtttactac atttttattg ccaaaagcgt tgtattttac ataaatttcg
     2041 cccacaatac caatacgtgg gacaaattct ttgtcagtac acatgctatt aaattcagca
     2101 gctgcttgtt ttaacagttt aagcatacct cgaatatctt tcttttcggc aaaaggcaaa
     2161 gctgcattga tgtatttatc cctaagagaa acagccccgc cttgcacttt ttgacgaaca
     2221 acagttgcat aatacatttt tgagatgcaa tctgcataaa gcatcgacac tgcggtaatt
     2281 gtaagtgctt ttcgccactt aacatcaaat ccgggttgcg ggttgagcag gctatctcct
     2341 gaagctattg aaatcacagg aatatcttca taaccagctg ctaccatagc cttttttatt
     2401 agcgaaaggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acaggaatat
     2521 cttcataacc agctgctacc atagcctttt ttattagcga aaggtaattg gtagcgcggc
     2581 attgtccgcc agtctgggta attccaatgg caatttcatc gcgattgtac ttcctggttt
     2641 gcaaggcttt tataatatcc ccaataacaa gggttgccgg gtaacatact tcgttgttgg
     2701 caaacttaag accaagttca gaagagtgta catctgaagg aggcaatgtt acaaagttat
     2761 agcccgccag tttaaatagt accggcacaa aaggcgagta atactcagaa aagaagggga
     2821 caagtattgt tctgtgtttg tcttcttcag taaatatttt atttttaatt aatggagtta
     2881 tagtaacttc cttgtcagaa gcaaatcgga gcgattcgat taacgaacgt atcctaaggc
     2941 gcagtgaccc aatgttatta acatcatcta ttttaagaat ggtatggctt ttaccatgac
     3001 gttttagtat ttcattgact tcatcaatga taaaggcatc cggtccacat ccaaacgaag
     3061 tcatttgtac aaggtggacg ttggcatttg ttttagcaac ccattctgca gctttcagga
     3121 tacgattggt gtaagcccat tgcataatgg tctgtacatt gtcaaaagta ttatgttctt
     3181 cacttctgac gaagtcttcc gagatgacat ctacgccaaa agcagaaatc atatcagata
     3241 tcttatgctg gattaaaggg tcggcatgat atggacggcc taccagcatg atgaccatat
     3301 tgtttcctac tgaatttttc tcgataatac gctggcaatt ttcttgtaat gagctaacgt
     3361 acatcctttg ggctgccaaa gctttaggta atgccttttc taccaaactt ttttcaatac
     3421 ccaatgtcat taaatacttt atgcaagcct ttgttaatag ttgctcattg ttaaaagcaa
     3481 tagttggggc atcaatacaa atattgtgtt tctccagaaa gggcatggag ctctttataa
     3541 catcagaata gcccgataca attggacaat tgaagctatt gggagtattt ttgtcttctt
     3601 tagactcata aaccacgtaa ggcatgaaaa tgcgatttgc ctttttatct atcaggtcaa
     3661 taatatggcc atg
//
LOCUS       DAOSPF010000136         3631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_25635_length_3631_cov_4.529642, whole genome shotgun sequence.
ACCESSION   DAOSPF010000136 DAOSPF010000000
VERSION     DAOSPF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25635_length_3631_cov_4.529642"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3631)
                     /locus_tag="PK252_14640"
     CDS             complement(<1..>3631)
                     /locus_tag="PK252_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15993.1"
                     /translation="ADESSVQIINVDRRIQASFTTDKTADCYPLQISTFTNNSSFVDP
                     NSRFLWEFGDGTSYLGATPANHTYNIQSAHDSTYYIKLTMTSQLNNKCVHSTTLPVTN
                     YAYVKASFILPSVGTICSNSPVLFQNTSSPGATHHYWDFIGDGTGNEEINSASFSHTL
                     VNNDVAVKAINVRLISRNEHACFDMAYQTVNVYPRITPSFATSVAEGCQPLSTTFTNT
                     SPGLGLPGNNFYWNFGGGFGAVNNAATVNHTFNNLTAKDSVFKIALVIDNGTCTDTVR
                     GTVKAYARIEADFNTVPSICSDIPFTIYRNQKYGEVNRYWTYSNGKRVNTLDNAAVIT
                     ETFVNTGNSPRKDVIKLVVTNNYPQCKDSVSHDVIVYPHAQASFSFQGGIDRGCEPVS
                     IGFVNSSKFKDTTGSTYVWDFGGLGSSINRDPAAFSFFNNEGTDKPYTVTLKEVTKYG
                     CEDVVSKTAIAYAHIEADFIPRKPAVCAPDPVELENTVLPGNKYYFWDFDGNGTVDGT
                     SGSSVVSHIYQNVSANPLTYNIVLKVQNEHPACEKTANSMVTIYPQVIADFTYTDSIG
                     CQPLTVNKFINKSNIKDAPGAKFYWSFDDGGTSFDKDPVGHQFVNTSNEIDTFRVRLQ
                     VKSSVYSCSGYITKNIIVYPFIKAGFFIPDANVCSNGKVTIYNSPQGNVTFNSWKIGD
                     EAEQNITSPLIYRAFSNQSLDTMFVKVRQLVTYQNNSDRCYDSTIQYVKVFPEVFADF
                     STDVDLGCQPLKVVYKNISNMLSRSGSIFEWSFGDGSSTTIKNPVTDTISHIYPNLTS
                     ISKQIFPKFKVTSREGCVDSSQTTLLVYPIIKAGFSVDTTEICSGESIGFKDASSKGT
                     SLRKWNLGDSLASNDLIINKRYINKNDNDNPKEHVVYLLSYFDYYQCKDSIAKTIKVY
                     PEVRAKIEALSSVKGCDSLKVTFANKTNKPAVNFNWDYKDGSNSNETGTVEHTFGNIT
                     SVDKHYWVKLDAASLQGCKSSDSIKITVYPYLYPNFGIPDVEVCHSEKIKIQDASKGG
                     IVFRKWDFDNDGIVDATNIASPFEIPTSNVGVKDSIRTIRLTIENHAGCQKNIAKNII
                     VHPKVVADFDYIPAGCTPFTPKFRSVSKNASVYEWDFGDGGSSTSANPGSKEYVNNTE
                     NDIQYTVKLKVFSKHLCKDSIEKPIVVAHRPVARIAVDKIIACSPFELTINNNTKTSG
                     STYKW"
BASE COUNT         1118 a          694 c          651 g         1168 t
ORIGIN      
        1 ttccatttat aggttgaccc tgaagttttt gtattgttat ttatggtaag ctcaaaaggc
       61 gaacatgcta ttattttatc aacagctata cgggctacag gcctgtgggc aacaacaatt
      121 ggcttttcta tgctgtcttt acacaaatgt ttagaaaaaa ctttcaattt taccgtatat
      181 tgaatatcat tttcagtatt atttacatat tctttagaac ctgggtttgc tgaggttgaa
      241 gagcccccat ctccaaagtc ccattcataa acactggcat tttttgaaac tgacctgaat
      301 tttggggtaa atggagtaca acctgccggg atataatcaa agtcagctac aacttttgga
      361 tggacaataa tgttttttgc aatatttttt tggcatccgg catggttttc aatggttagc
      421 ctgatagtcc ttatactatc ttttacccca acgttagaag ttggaatttc aaaaggagaa
      481 gcgatattag tggcatcaac aattccatca ttgtcaaaat cccattttcg gaatacaatg
      541 cccccttttg atgcatcctg gatttttatt ttctcactat ggcaaacttc aacatcagga
      601 atgccaaaat tggggtacaa ataaggataa acagttattt taattgagtc tgaagatttg
      661 caaccttgta atgaggcagc atctaacttt acccaataat gtttgtcaac actggtaata
      721 tttccaaaag tatgttcgac tgtacctgtt tcattagagt tactaccatc tttataatcc
      781 cagttaaaat tgactgccgg cttatttgtt ttgtttgcaa acgtaacctt tagtgaatcg
      841 caacctttga cacttgacaa cgcttcaatt ttagccctta cttcaggata cactttaatt
      901 gtttttgcta tagagtcttt acattgataa taatcaaaat atgataacag ataaactacg
      961 tgctctttgg ggttatcgtt atcgttttta ttgatatatc gtttgtttat tatcaaatcg
     1021 tttgaagcca gcgaatcgcc caggttccat ttccttaaag aagtcccttt agaagacgca
     1081 tccttaaagc caatactttc gccagaacat atttcggtgg tatcaaccga aaatccggct
     1141 ttgataattg gatataccaa tagtgttgtt tggctgctat ccacacaacc ttctcttgat
     1201 gtaactttaa atttagggaa aatctgtttg ctaatgcttg tcaaattggg atatatatga
     1261 gatattgtat cggttacagg attttttatg gtggtggagc tcccgtctcc aaaactccat
     1321 tcaaaaatgc tgccacttct gctcaacata ttggaaatgt tcttatatac aacctttaac
     1381 ggttgacaac ctaaatctac atcagttgag aaatctgcaa atacttcagg aaatactttt
     1441 acatattgaa tagtgctatc ataacacctg tcactgttat tttgataggt gaccaattgt
     1501 cttactttaa caaacatggt atcaaggctt tgatttgaaa atgccctata tatcaaagga
     1561 gaagtaatgt tttgttctgc ttcatctcca attttccagc tgttgaaagt tacatttccc
     1621 tgtgggctat tgtaaatggt aacttttcca tttgaacaaa cattggcatc cgggataaaa
     1681 aagcctgctt ttataaatgg atatacaatg atatttttgg taatataacc agaacaacta
     1741 taaacactcg attttacctg caaccgaacc ctgaaagtgt ctatttcgtt gcttgtatta
     1801 acaaattgat gcccaacagg gtctttatca aaagaagtgc ctccatcatc aaaactccag
     1861 tagaacttag ccccgggagc atctttaata ttgctcttat tgataaattt atttaccgtt
     1921 agtggctggc aaccaatgct atcggtatat gtaaagtctg ctatcacctg cggataaata
     1981 gtaaccatac tgttagcagt tttttcgcaa gcaggatgtt cattttgtac ttttaatact
     2041 atattgtagg tcagaggatt agccgaaaca ttttgataaa tatgagaaac tacgcttgac
     2101 cctgaagtcc catcgacagt accattgcca tcaaaatccc agaaataata tttatttcct
     2161 ggcaatactg tattttctaa ttcaacaggg tctggggcac aaaccgcagg cttgcgcgga
     2221 ataaaatctg cttcaatatg ggcataagca atagctgttt ttgacacaac atcttcacat
     2281 ccatatttgg ttacttcttt taaagtgaca gtataaggtt tatcagtgcc ttcattattg
     2341 aaaaatgaaa aagctgcagg gtcgcggttg atagatgaac ctaatccgcc aaaatcccat
     2401 acataagtag aacctgtggt atctttaaac ttagaactat tgacaaaccc aatagaaact
     2461 ggctcgcaac cccgatcgat gccgccctga aatgaaaacg aagcttgagc atgaggatat
     2521 acaataacat catggcttac cgaatcttta cattgaggat aattattggt aaccacaagt
     2581 ttaataacat ctttacgggg actattgccg gtattaacaa atgtttcagt aattacagca
     2641 gcattatcaa gggtattaac acgtttgcca ttgctatatg tccagtagcg gttaacctca
     2701 ccatattttt ggtttcggta aatagtaaaa ggtatatcag aacatattga aggaacagtg
     2761 ttaaaatctg cttcaatcct ggcataagct tttactgtgc ctctaacagt atcagtacaa
     2821 gttccattgt caatgaccag tgctattttg aaaaccgagt ctttggcagt aaggttatta
     2881 aatgtatgat taactgttgc agcattatta acagccccaa atccgcctcc aaaattccag
     2941 taaaagttat tcccgggcaa accaagtccg gggctggtat tggtaaaggt tgtagaaagt
     3001 ggttgacagc cttctgcaac cgaagtggca aagcttggcg taatacgcgg atatacatta
     3061 accgtctgat aagccatatc aaaacaagca tgctcatttc ttgaaataag ccttacattg
     3121 attgctttta ccgcaacatc attatttacc aaagtatgac taaaagaagc gctgtttatt
     3181 tcttcatttc cggtaccatc ccctatgaaa tcccagtaat ggtgagttgc tcccggcgat
     3241 gatgtattct gaaacaagac ggggctgttt gaacaaatag tcccaaccga tggtaaaata
     3301 aaggaagctt ttacataggc ataattagta acaggcaatg tagttgaatg aacacattta
     3361 ttgttcaatt gagaagtcat tgtcaacttg atataatagg ttgaatcatg agccgactgg
     3421 atattgtatg tatggtttgc aggtgttgcc cccagataac tggttccgtc tccaaactcc
     3481 cataaaaaac gtgaattagg gtcaacaaaa cttgaattgt tagtaaaagt acttatttgc
     3541 aatggataac aatcagctgt tttatctgta gtaaacgagg cctgaatgcg cctgtcaaca
     3601 ttgattattt gaacagaaga ctcatcagca c
//
LOCUS       DAOSPF010000137         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_30275_length_3192_cov_6.177558, whole genome shotgun sequence.
ACCESSION   DAOSPF010000137 DAOSPF010000000
VERSION     DAOSPF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30275_length_3192_cov_6.177558"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PK252_14645"
     CDS             <1..1271
                     /locus_tag="PK252_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15994.1"
                     /translation="TAICLLCVPVLMGQQNWDDHDRSDRYTCRDFASNYLNSCEKDAI
                     IFTNGDNDTFPLWYAQEVEGIRTDVRVVNLSYLGADWYLEQMHRKAYESEPLPFSLTR
                     EKVLQGKRDIAYLINQIKEPIELKDGIEFLASDDPKTKQYGNYAERLDYIPGKTFKVT
                     VDTTAVFKNKYVPENMKAKVTNQMQWTIEKNYLTKADILVLDLIAHNNWKRPVYFAVT
                     VSHDNYLNLEPYMQINGLTYKVIPVKSEVQMGDIMNIDIDTTYNNMMNKFKWGGINNP
                     KVYLDENILRMLSNIRNSFAKLADALILANKKDSALKVVDRAMELMPDKCVPYNYFTM
                     PYVDLYYKLNQPAKAQAISERLAVIAEQELTYIFRLKPEDRVLLDYDMKVNLRILQQL
                     SMIEKQYGNTEKANKLEMLFQEFYGKFANM"
     gene            1598..2551
                     /locus_tag="PK252_14650"
     CDS             1598..2551
                     /locus_tag="PK252_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT15995.1"
                     /translation="MNQLLRPQKFTYGLLALAGVVLFSAKSVFAKMIYAYNVHPVAML
                     YLRMMVALPFLLAFYFWYENKYPQKKAHWRDILQVIGISLIGYYLSGILDFIGLLYVE
                     AAIERLILFLYPTMVLVLSILFLRKEAKLNQFIAMIVSYIGLLVAFADKLTMSNTSMF
                     WFGVVLIVLSSFLYAIFLTLADKLIDRLGSIRFTTIACLTMTASIVIHAFITGKAHFV
                     GYPSQVYLYSFIMGVFSTVVPVFMFNYAIEKLGSTNVSIISCAGPICTLMYSALLIDE
                     PITLYQIVGTLIVMAGIMFVYKSKTAPVEVRSSDYRRKEND"
     gene            complement(2585..>3192)
                     /locus_tag="PK252_14655"
     CDS             complement(2585..>3192)
                     /locus_tag="PK252_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT15996.1"
                     /translation="IFDLGGVIVEIDYQITIGMFKKLGISDFDSLYGQYKQAHLFDGF
                     DKGHVSPEQFREGLSQFLHKSIDKSDFDAAWNSMIKQLPAQNIVLLDILKNKYNTFLL
                     SNTNEIHLEYFFRMIYDVHKVNQFESLFNKAYYSCRIGMRKPDREIYEFVLKDSGLKA
                     EESLFIDDTSINLIPAEEVGIKTFLMPKGRLLYQVINACSL"
BASE COUNT          968 a          577 c          610 g         1037 t
ORIGIN      
        1 tcactgctat ttgcctcttg tgtgtcccgg tattgatggg ccaacaaaac tgggacgacc
       61 acgatcgttc agaccgatat acttgtaggg actttgcgtc aaattatctt aattcttgcg
      121 aaaaagatgc cattatattt accaacgggg acaatgatac attcccgctt tggtatgccc
      181 aggaagttga aggcatccgt acagatgttc gtgtagtcaa cctaagttat ttgggcgccg
      241 attggtatct tgaacaaatg caccgtaaag cttacgagtc agaacctctg cccttcagcc
      301 ttacccgtga aaaagtactt cagggtaaac gtgatattgc ataccttatc aatcaaataa
      361 aagagcctat tgaactgaaa gacggtattg aattccttgc cagcgacgat cctaaaacca
      421 agcaatacgg caactatgcc gaacgtttgg attatatccc tggtaaaaca ttcaaggtta
      481 ctgtagatac cacggcagtt ttcaaaaaca aatatgtccc tgaaaacatg aaagctaaag
      541 ttaccaatca gatgcagtgg actattgaga aaaactatct taccaaagcc gatattttag
      601 ttcttgacct cattgcccac aataactgga agcgaccggt atattttgcc gtaacagtat
      661 cgcatgacaa ttatctcaac cttgaacctt atatgcagat caatgggttg acttataaag
      721 ttattcctgt gaaatctgag gttcagatgg gcgacattat gaatattgat attgatacca
      781 cctataacaa catgatgaac aagtttaaat ggggaggtat caacaatcct aaggtttatt
      841 tggacgagaa catcctccgc atgctttcaa acatccgcaa cagttttgca aagttggccg
      901 atgcacttat attagcaaat aaaaaagatt cggcattgaa ggttgttgac cgtgctatgg
      961 aactaatgcc tgacaaatgt gtaccatata actactttac catgccttat gttgacttgt
     1021 actacaagct caaccagccg gcaaaggcac aggcaatatc cgaaaggttg gctgttattg
     1081 ccgaacaaga gcttacttat atttttaggt taaaacctga agacagggta ttgctggact
     1141 atgatatgaa ggtaaacctt cgtatattac aacaattgtc aatgattgaa aaacaatatg
     1201 gcaatacaga aaaagctaac aagctggaaa tgttgttcca ggaattttac ggcaagtttg
     1261 ccaatatgta aaatgatata aaactatata gaaggcattg ggttttccta atgccttttt
     1321 tatttttaag cccatttttt gttttcagaa aaaacgattg tggcataaat tttgttaaac
     1381 aaaatactat cgttaattta acaaacataa atttctgatg aaaaattgta caaagcatac
     1441 caggtcaatt tcatggttcg cagctgataa gaactcgcga aggcttttga tatgtgcttt
     1501 ttacaataat aaatgatatt attcatgact aaaaaagctt aaaatactga agccttccaa
     1561 aaacggaagg cttttttgtt tattaactcc taacattatg aaccagttat tacgaccaca
     1621 aaaattcact tacgggctct tggcattagc cggagttgtg ctgttttctg caaaatcagt
     1681 ttttgccaaa atgatttatg cttacaatgt tcatcctgta gccatgcttt atctgcggat
     1741 gatggtagca ttgccttttt tactggcctt ttatttttgg tatgaaaata agtatcctca
     1801 aaagaaagct cattggagag atatattgca agttataggg ataagcctta tcggctatta
     1861 cttatcagga atacttgatt ttattggtct tttatatgtt gaggctgcta tcgaacgatt
     1921 gatattgttt ttatacccta ctatggtgtt ggtgctttct attttgtttt tgcgcaaaga
     1981 agctaagctc aatcagttca ttgcaatgat tgtttcttac atagggttgc tggtagcttt
     2041 tgcagataaa ctgacgatgt caaatacctc catgttttgg tttggagtgg tactgattgt
     2101 attaagttct tttctttacg ctatatttct tactttggcc gataagctta tcgacaggtt
     2161 aggttcaata aggtttacaa ccatagcatg tcttacaatg actgcaagta ttgtcattca
     2221 tgcgtttatt acagggaaag cgcattttgt aggctatcca tcacaggtat atttatatag
     2281 ctttatcatg ggcgtgtttt caacagtagt acctgttttt atgtttaatt atgctattga
     2341 aaagcttggt tctactaacg tttcgatcat tagttgtgca ggccctattt gtactctaat
     2401 gtattcggcc cttttgatag atgaaccaat tacgctttat caaattgttg gtacattgat
     2461 agtcatggct ggtattatgt ttgtttataa aagcaagact gcaccagttg aagtcagaag
     2521 ttcggattat agaaggaaag agaatgatta gtgtttaggc ctttatatcg atgcgtgtag
     2581 cctgttatag gctgcacgca tttattacct ggtaaagaag acggccttta ggcatcagga
     2641 atgtctttat tccaacttct tctgcgggaa tgaggttaat agacgtatcg tcaataaaaa
     2701 ggctttcttc ggctttcagc ccgctatctt tcagcacaaa ctcatatatt tccctatctg
     2761 gcttgcgcat tcctattcgg catgaataat acgctttatt gaaaaggctt tcaaactggt
     2821 tgactttatg tacatcatat atcattcgaa aaaaatactc gagatgaatt tcattggtat
     2881 tactcaaaag gaaagtattg tacttatttt ttaatatgtc cagcaaaacg atattctgag
     2941 ctgggagttg ctttatcatc gagttccacg ctgcatcaaa gtcagattta tcgatagatt
     3001 tatgaaggaa ttgtgaaagt ccttctctga attgttcggg agaaacatgc cccttgtcaa
     3061 agccatcaaa caaatgggcc tgtttatatt ggccatagag cgagtcaaaa tctgaaatcc
     3121 caagtttttt gaacattcca atggttattt gatagtcaat ttcaacaatt accccgccta
     3181 agtcgaatat aa
//
LOCUS       DAOSPF010000138         3129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_31125_length_3129_cov_6.524398, whole genome shotgun sequence.
ACCESSION   DAOSPF010000138 DAOSPF010000000
VERSION     DAOSPF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31125_length_3129_cov_6.524398"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..788
                     /locus_tag="PK252_14660"
     CDS             258..788
                     /locus_tag="PK252_14660"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HOT15997.1"
                     /translation="MPNNEILPAAEFQALPLENIIAAPLLGVIKAQAAAAAATQTYIN
                     SLIKDGKPLTVDLSLNVAQNGVNKSVEIKAPLLSMVPVPHIRIDSFTTHFKYEISQVS
                     KSTDELSYGANIDADVTKNPFFNLSIKGNVSSKSADESVMNRSGMLEITVHASEAPIP
                     EGLARLLSVLSKALPE"
     gene            791..1177
                     /locus_tag="PK252_14665"
     CDS             791..1177
                     /locus_tag="PK252_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15998.1"
                     /translation="MSRNLSEIIRSLCYELVKTDASFVIAQRTAFFDLLRDQYHDMFL
                     NDHHFLGLQNLSFSFYLKPVKQPWWKRIFRRKTRNEKMLFFEMASENTPGGVKCEVTF
                     SRTDKTFVDEIKIDGKVVKNDQVYVN"
     gene            1167..1910
                     /locus_tag="PK252_14670"
     CDS             1167..1910
                     /locus_tag="PK252_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT15999.1"
                     /translation="MLTKLENGWYCFGNGKPWRLKAPYFLCEVGAEGTIPDYSNMPSV
                     MAKLRRIKAFYTGKLKLGNLDEHGNGIDILVANSMTESLGTVPSPLNYKDLRKAFDNI
                     VGSDVGSRLDEIVRLISRSAGAKYLERREPGYIDPIRTPGRVSLGSHHILISTALQSK
                     GKLNSSDKEAQIIDLVLRLPSESIFAAQLAIDYLNSRYSKHQNHPPLIAATYNAGSPR
                     LTSANAWNLVQYGEHIDRWIAYYNTSRKI"
     gene            complement(2009..2575)
                     /locus_tag="PK252_14675"
     CDS             complement(2009..2575)
                     /locus_tag="PK252_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT16000.1"
                     /translation="MSIFALFLLSIALSFDTFAVSITCGLIERHLRFWPAARIAVYFA
                     AFQALMPVLGYFLGLSFRGYVQGVDHWIAFGLLFVVGGKMIVESLKKGEIECFDIHSR
                     KVIVTLAVATTIDAFAVGITFALLEINLFISSVIIGIVTFTVAMLGMLLGKKIGEYYG
                     KKVGIAGGLILVAIGTKIIIEHLGVRVF"
     gene            complement(2617..>3129)
                     /locus_tag="PK252_14680"
     CDS             complement(2617..>3129)
                     /locus_tag="PK252_14680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOT16001.1"
                     /translation="GNGGKTLHLASLMQNKGKIVAMDTQEEKISTLKSRVAKAGADIV
                     EARLIEGTKSTKRLSQSFDRVLIDSPCSGSGTIRRNPEIKWQMNLQRLEKIKNIQNIV
                     LKNYANMAKPGGLIVYATCSILPEENHERVEAFLESQKGAYELVKEQQITTSQNGFDG
                     FYMAQIRRLK"
BASE COUNT          936 a          705 c          581 g          907 t
ORIGIN      
        1 aaaaatgctt cgcctaaagg cgagggattt cacccatccc agaattataa attaatttct
       61 tcattgtttt tttgattttt ggttaataat gggacaatat taagcatttt cttcataatt
      121 actataccta atctttatta gtgctttttt ttcttaattg acgaatttcc tcattttcaa
      181 attttcaaat tcttcctttg cttttttcat attttcaatg tagctttatt accgttaaac
      241 cttcaaatca aagctatatg ccaaacaacg aaatccttcc tgcagccgaa tttcaggctt
      301 taccattaga aaacattatt gcagcgcctc ttttgggggt tattaaagcg caagctgctg
      361 ccgctgctgc tactcaaaca tacatcaatt cgcttatcaa agacgggaag ccccttacgg
      421 tagatttaag cctaaatgtt gctcaaaacg gggttaacaa atcagtagaa ataaaagccc
      481 ctttgctttc tatggttccg gtgcctcata ttcgtatcga ctcatttacc acacatttca
      541 agtatgaaat atctcaggta tcaaagtcaa ctgacgagtt gagctacggg gcaaacatcg
      601 atgctgatgt tactaagaac ccattcttta acctttcgat aaaagggaat gtatcgagca
      661 aatcagctga tgaatcagtg atgaaccgca gcgggatgct cgagataaca gttcatgcca
      721 gcgaagcccc tatccctgag gggttagcac gcttactttc tgtgttgtca aaagctttgc
      781 ccgaataata atgtcccgca acctttccga aattattcgc agtctttgct atgagttagt
      841 aaagaccgat gcaagttttg tcattgccca gcgcacagct tttttcgacc tgctcaggga
      901 ccaataccat gacatgtttc tcaacgacca tcattttctt gggttacaaa accttagttt
      961 tagcttttat ctaaaacctg ttaaacaacc ctggtggaaa aggattttca gacgaaaaac
     1021 gagaaatgaa aaaatgttat tctttgaaat ggcctcggaa aataccccgg gaggagttaa
     1081 gtgcgaggta acattcagcc gcactgacaa aacttttgta gatgaaataa agattgacgg
     1141 caaggtggtg aaaaacgacc aggtatatgt taactaaact tgaaaacggt tggtattgct
     1201 tcgggaacgg caagccgtgg agacttaaag ccccttattt tttgtgtgaa gttggggcag
     1261 aaggcacaat ccctgactat tccaatatgc cttcagtcat ggctaaacta agacggataa
     1321 aagcttttta tactggcaaa ctgaaactgg gcaacttaga tgaacatggc aatggtatcg
     1381 atatcctggt agccaattct atgaccgaat ctctgggtac agtcccatct ccgcttaatt
     1441 ataaagattt gcgaaaagct tttgataaca ttgttggcag cgatgtcggg agccggcttg
     1501 atgagattgt ccggcttatc tcccgttctg caggcgcaaa atatctggag cgccgtgaac
     1561 ctggatatat agacccaata aggactccgg gaagagtgtc gctgggaagc catcatatac
     1621 ttatctctac tgctttgcaa tcaaaaggca agctgaacag ctccgataaa gaagcacaaa
     1681 taattgactt agtattacgg cttccatccg aaagcatttt tgcagcacag ctggctattg
     1741 attatctgaa ctccagatac tcaaaacatc agaaccatcc ccctcttatt gcagctacct
     1801 acaatgcagg ttctccacgt ttgacctctg caaatgcatg gaatttggtt caatacggag
     1861 agcatatcga caggtggata gcttattaca atacttcaag gaagatataa tcaaaactca
     1921 ccccaacccc tctctttcaa gagaggggct aagatacgaa gctcccaagt cccttctcta
     1981 aatagagaaa ggatttatgg ataagttttt aaaatacccg aacccccaag tgctctataa
     2041 taatctttgt tccaatagcc acaagaataa gccctccggc tatgccaact tttttaccat
     2101 aatactcccc tatttttttc ccaagcaaca tacccagcat agccacagta aatgttacaa
     2161 tgccaataat aacggaagaa atgaaaaggt tgatttcgag caaagcaaat gtaatgccca
     2221 cagcaaaagc atcgatggta gttgcaacag ctaaagtaac aattactttt cggctgtgaa
     2281 tgtcgaaaca ttctatttca cctttcttga ggctttcaac aatcattttg ccccctacaa
     2341 cgaaaagtag cccgaaagca atccaatggt caactccctg aacatatccc ctaaaactca
     2401 atcccagaaa atatcccaat actggcatta atgcctgaaa tgctgcaaaa taaactgcaa
     2461 tacgtgcagc cggccaaaag cgcaaatgcc gttcgattag tccacaggta atagacactg
     2521 caaacgtgtc aaatgaaagg gctatggata gtaaaaacag ggcgaatatg ctcataggtt
     2581 aattagctaa tttgaaaatt tgaaaatgag taaattttat ttcaaccttc gtatctgtgc
     2641 catgtaaaac ccgtcaaagc cattttgcga agtcgttatc tgttgttctt tgaccaactc
     2701 atacgcacct ttttgcgatt ccagaaaagc ctcaacacgt tcgtggtttt cttcaggcaa
     2761 tatgctgcat gtggcataca caatcaaccc tccgggtttg gccatgtttg catagttttt
     2821 caaaacaata ttttgtatgt tctttatttt ttccaacctt tgaaggttca tttgccactt
     2881 aatctccggg ttacgcctga tagtaccact tcccgaacat ggcgaatcaa taagcactcg
     2941 gtcaaagctt tgcgaaaggc gtttggttga cttagtccct tctatcaaac gagcctcaac
     3001 aatatcagcc cccgcctttg ctacacggct tttcaacgtg ctaatctttt cttcctgtgt
     3061 atccatcgca actatcttgc ctttgttttg catcaggctt gccaggtgaa gcgttttacc
     3121 gccattacc
//
LOCUS       DAOSPF010000139         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_34155_length_2911_cov_6.550070, whole genome shotgun sequence.
ACCESSION   DAOSPF010000139 DAOSPF010000000
VERSION     DAOSPF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34155_length_2911_cov_6.550070"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2728)
                     /locus_tag="PK252_14685"
     CDS             complement(<1..2728)
                     /locus_tag="PK252_14685"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOT16002.1"
                     /translation="MIKEQQIEDSLIVKLADLKYIYRSDIRDRISLENNFREKFQALN
                     RVNLTDAEFARLRDEIITSDVFAASKRLREINTFTREDGTPLHYTLVNIKEWCKNEFE
                     VIHQLRINTNNSYHRYDVILLINGIPVVQIELKSLEVSPRKAMQQIVDYKSDPGNGYT
                     NSLLCFMQLFIVSNRTNTYYFANNNAVHFSFNADEQFLPIYQFADESNKKIMHLDDFA
                     EKFLSKCTLARMISKYMVLVASEQKLLVMRPYQIYAVKAIVDCIHQNRGNGYIWHTTG
                     SGKTLTSFKASTLLKDNPDIEKCLFVVDRKDLDRQTREEFNKFQEGCVEENTNTETLV
                     RRMLSDDYANKVIVTTIQKLGLALDPSYRRDMSRFNNNYKERLQPLKNKRIVFIFDEC
                     HRSQFGENHKAIKEFFPNAQLFGFTGTPIFEDNANYKQIDGTVGSYVTTKDIFQNQLH
                     CYTITHAIEDRNVLRFHIDYFKPEKNATIGSTEHKKAVVQAILKKHDAATHNRRFNAL
                     LATASINDAIEYYELFKSVETLHATSQPDFIPLNIACVFSPPADGNRDVQQWQEDLEQ
                     EKADNKIEPEKKKKALEAIINDYNKQYGTNHKIFEFDLYYQDVQQRIKAQQFSNADYP
                     HKNKIDITIVVDMLLTGFDSKYLNTLYVDKNLKYHGLIQAFSRTNRVLNDTKPYGNIL
                     DFRQQQQQVDEAIALFSGEAGERAKEIWLVEPAPKVIEKYEQAVKAMEKWMHDKNMVA
                     EPQEVYNIKGDTARIEFINRFKEVQRLKTQLEQYTDLSEEQKAKIEQLIPEDQLRSFR
                     SSYLEIARQLKEIQQKEGNKAPENIQQLDFEFVLFASALVDYDYIMNLIAKYTQQQPK
                     KQKMTRDQLIGILSSSANLMEERDDIAEYINTLEVGKALNEQQIKDGYQIFKE"
BASE COUNT          675 a          566 c          642 g         1028 t
ORIGIN      
        1 cttccttaaa tatctggtaa ccgtctttta tttgttgctc gttgagtgct ttgcccactt
       61 ccaatgtatt gatgtattca gcaatatcgt cgcgttcttc catcaggttg gcgctgctgc
      121 tcaggatgcc aatcagctgg tcgcgggtca ttttttgttt tttgggttgc tgctgggtgt
      181 atttggcaat caggttcatg atatagtcat aatctaccaa agcagaggca aaaagcacaa
      241 attcaaaatc gagctgctga atattttcag gggctttgtt accttctttt tgctgaattt
      301 ccttgagctg tctggcaatt tccaggtacg aacttcggaa cgaacgcaac tggtcttcgg
      361 gtataagttg ttcgatttta gctttttgtt cttccgaaag gtcagtatat tgttcgagct
      421 gggttttgag acgttgcact tctttaaaac ggttgatgaa ctctatacgt gctgtatctc
      481 ctttaatgtt atacacttct tggggttctg ccaccatgtt tttgtcgtgc atccattttt
      541 ccatagcttt tactgcctgc tcgtattttt caataacttt cggtgctggc tctaccagcc
      601 agatttcttt ggcccgttct cctgcttctc cgctaaatag ggctatggcc tcatctacct
      661 gttgctgttg ttgcctgaaa tcgaggatat ttccgtatgg cttggtatcg ttcaacacgc
      721 ggttggttcg gctaaaagcc tgaatcagtc cgtgatattt aagattttta tcaacataca
      781 gcgtgttgag gtatttactg tcgaaaccgg tgaggagcat atccaccaca atggtgatgt
      841 caattttatt tttgtgcggg tagtcggcat tgctaaactg ctgagctttt atgcgttgct
      901 gcacatcctg ataatacaag tcgaactcga aaattttatg gttagtgcca tactgtttgt
      961 tgtaatcgtt gataatggct tctaatgctt ttttcttctt ttcgggttca attttgttgt
     1021 cggctttttc ttgctctaaa tcttcctgcc attgctgaac atctctattt ccatcggcag
     1081 gcggagaaaa cacacaggca atatttaaag gaatgaaatc gggttgagac gttgcatgca
     1141 acgtctcaac actcttgaat agctcgtaat attcaatggc atcattgatg gaggcggttg
     1201 ccaataatgc gttaaaacgt ctgttatggg tggcggcgtc gtgttttttc agaatagctt
     1261 gcactactgc ttttttgtgt tcggtgcttc caatggttgc atttttttcg ggtttgaagt
     1321 agtcgatatg gaatcgcaat acgtttcggt cttctatggc gtgggtaatg gtatagcaat
     1381 gcagctggtt ttgaaagata tctttggtgg taacataaga acccacggtt ccgtcaattt
     1441 gcttgtagtt ggcattatct tcaaaaatgg gcgtacctgt aaaaccgaat agttgtgcat
     1501 ttgggaaaaa ctccttaatg gctttatggt tttcgccaaa ctgcgagcgg tggcactcgt
     1561 caaaaatgaa aacaatgcgt ttgtttttta atggctgtaa gcgctcttta tagttgttgt
     1621 tgaaacgcga catgtcgcgt ctgtaggagg ggtctaaagc caaacccagt ttctgaatgg
     1681 tagtaacaat taccttgttg gcatagtcgt cggacagcat gcgccttacc agagtttcgg
     1741 tattggtgtt ttcttccacg catccttcct gaaacttatt aaattcctcc cgggtttgcc
     1801 ggtcaaggtc tttgcggtca accacaaaca aacatttttc aatgtcgggg ttgtctttta
     1861 gtagggtaga agccttaaaa gaagtcaagg tttttccgct tcctgtggta tgccagatgt
     1921 atccgttacc tcggttttga tgtatgcaat ctacaatggc ttttacggca taaatctgat
     1981 aaggccgcat gacaagcagt ttttgttcgc ttgccaccaa aaccatgtat ttgctaatca
     2041 tccgggcaag tgtgcatttg ctcagaaact tttcagcaaa atcgtctaag tgcataatct
     2101 ttttattgct ttcatcggca aattgataga tgggcaaaaa ctgttcgtcg gcattaaagc
     2161 tgaaatgcac tgcattgtta ttggcaaagt agtaagtatt cgtgcgatta ctaactataa
     2221 acaattgcat gaagcatagc agcgaattgg tgtagccgtt tcccgggtcg cttttgtagt
     2281 ccacaatttg ctgcatggct ttgcgtgggc ttacctccaa acttttcagt tcaatttgaa
     2341 cgacgggaat tccatttata agcagaatta catcgtagcg atgatagctg ttatttgtat
     2401 taatgcgtag ttgatgtatg acttcaaact cgtttttgca ccattcttta atgtttacta
     2461 atgtgtagtg caaaggcgtt ccgtcttcac gtgtaaaggt gttgatttcc ctaaggcgtt
     2521 ttgaggcagc aaacacatcc gaagtaatga tttcgtccct cagtcgggca aattcggcat
     2581 ctgtaaggtt taccctgttt agtgcctgaa acttttcacg gaaattattt tcaagcgaaa
     2641 ttctatccct gatgtctgac ctgtaaatgt atttaaggtc agcgagcttg actattaaac
     2701 tatcctctat ttgttgttct ttaatcatag aaaaatgatg tccgtcaaag ttacttattt
     2761 attttgttaa tcggtctttg cgttggcaaa aatatgctct ttggattttg cttgtgtatc
     2821 gggctgtgtt tcgatagcta tcgggaggtt tactggtctt gttgatgttt cgttttccag
     2881 tcctttcaca atggtttatt tttcattttt t
//
LOCUS       DAOSPF010000140         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_36465_length_2769_cov_10.201179, whole genome shotgun
            sequence.
ACCESSION   DAOSPF010000140 DAOSPF010000000
VERSION     DAOSPF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36465_length_2769_cov_10.201179"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK252_14690"
     CDS             <1..969
                     /locus_tag="PK252_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOT16003.1"
                     /translation="NILNNRYALTQIQEYIGLPGMLFEGEFRFTKEMVANFFEIDIST
                     IDRYLAKYEAELKHNGYILTKGKQLKDFKLQFAHLIDKASKTRQLGLFNFRALLNLSM
                     LLVESEKARHLRSKILDIVIHTINEKTGGGTKYINRRDEDYLINAVREHKYRKDFTNA
                     LNQYVDLGNYKYSLYTDKIYQAIFRENAKEYKQILKLADNENARDTMYAEVLKLIASF
                     EAGIAFEIEKKAKELNRKLLSFEVDQIVKDFAEHPSQLPHIEDARIKMASRDNFFRDA
                     FHHKLKEYITSVSPADFDKFIGDRSVDFEQQLKEAEDVFKRLKDSE"
     gene            969..1430
                     /locus_tag="PK252_14695"
     CDS             969..1430
                     /locus_tag="PK252_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOT16004.1"
                     /translation="MPIVYITDINELVSLHQKTIEVSGGGTVGILSLNSLCAALDHIQ
                     NDDYYPTFEDKLTHLFYVANKSHSFQDGNKRIAITVGSIFLLKNGYINAAKEFLYRME
                     IISYHVAASNIDKELLHEIITSILNEADFSEELKLKIACAISQDGQENEEY"
     gene            1437..>2769
                     /locus_tag="PK252_14700"
     CDS             1437..>2769
                     /locus_tag="PK252_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16005.1"
                     /translation="MAEQNLILNILTFNHPVVKKDFAFYTEKKNGCLPINHYEFPLNI
                     EEIIPDAKTQKLQQLYTDFENTSDKEALISVDLTKCTRFAKHYYAWLIYNHFKTVADV
                     VNSNFVRDLEVWFYDKKESSTEYNAYKVFTLRVQIARNAATPELIISYDGISLVHKKS
                     ILDLDIETTMFKRVVYEKQIYKYDKLPDEAKYHWENVYPILSNTLKYALNIPVQPKRN
                     GTNRYKVYKSNLDYFIGTFIKSKQFSSIIALNDENYFKVETIRIKNTTPGSNRLQFGQ
                     DKTDIVPYTGLIGNGPYSHCPFAHVNMFFICHESEKDKANLLYTYFKDGYKNFPKLNQ
                     LLKVPVNIIKADQITFTNNENPLPEIRQSLFSKSFDPNIRYIAIYLSPFGKNHPDTTK
                     RQYYFQVKEELLKYGITSQVIETTKISDPNYNYSLVNIGVAILAKLNGVPWR"
BASE COUNT          994 a          525 c          464 g          786 t
ORIGIN      
        1 aacattctga ataaccgcta tgctttaacc cagattcagg aatacatcgg tttacccgga
       61 atgttatttg aaggcgaatt tcgttttacg aaagaaatgg ttgccaattt ctttgaaatt
      121 gacatttcta caattgacag gtatcttgct aaatatgaag ctgaattaaa gcataatgga
      181 tacattttaa ccaagggtaa acaactgaaa gacttcaaat tacaatttgc tcacctcatt
      241 gataaggcga gcaaaactcg tcagcttggt ttattcaatt ttagggcact gttaaattta
      301 tctatgttac ttgtcgaaag tgaaaaagcc cgtcatttaa gaagtaaaat cctcgatatt
      361 gtaattcata cgattaacga aaaaaccgga ggaggcacaa aatatattaa tcgcagggat
      421 gaagattatt taataaatgc tgtcagggag cacaaataca gaaaagattt cacgaatgcc
      481 cttaaccaat atgttgacct cggtaattat aagtactctc tatatactga caagatttat
      541 caggcaattt ttcgtgaaaa tgctaaagag tataagcaaa ttctgaaact ggcagataat
      601 gaaaatgcaa gagacacaat gtatgcagag gttttaaaac taatagcatc atttgaagcg
      661 ggtattgctt ttgaaattga gaaaaaagct aaagaactaa accggaaact actgtctttt
      721 gaagttgacc aaattgtaaa agatttcgca gaacatccct cacaactacc ccatatcgaa
      781 gatgccagaa taaaaatggc ttcccgtgat aattttttca gagatgcatt ccatcataaa
      841 ctcaaagagt acataacatc tgttagccct gctgattttg ataaattcat tggcgaccgc
      901 agtgttgatt ttgaacaaca gttaaaggag gcagaagatg ttttcaaacg cttaaaagac
      961 agtgaataat gccaatagtt tacattacag atattaacga gcttgtctcc ttacatcaga
     1021 aaaccattga ggtaagtggc ggtggtactg ttggcatact tagccttaac tcgctatgtg
     1081 ccgcactcga tcacattcag aacgatgatt attaccctac gtttgaagac aaactaacac
     1141 atttgtttta tgtagccaat aaaagtcata gttttcagga tgggaataaa agaattgcaa
     1201 ttacagttgg aagtattttc cttttaaaaa atggctatat caatgcagca aaagaatttc
     1261 tgtatcgtat ggaaataatc agttatcacg ttgcagcaag caatattgat aaagaattac
     1321 tgcatgaaat tattacctct attctaaatg aagcagattt ttcagaagaa ttaaagctaa
     1381 aaattgcctg tgcaatttct caggatgggc aagaaaatga agaatattaa acaaatatgg
     1441 cagagcaaaa tcttatacta aacattctta ctttcaacca tccggttgta aagaaagatt
     1501 ttgccttcta caccgaaaag aaaaacggct gtttgcccat taatcattac gagttccctt
     1561 taaacattga agaaattatc cctgatgcaa agacacaaaa acttcaacaa ctttataccg
     1621 attttgaaaa taccagcgac aaggaagcct taatatccgt tgaccttaca aagtgtacaa
     1681 ggtttgcaaa acattattac gcatggctca tttacaacca tttcaaaacc gttgcagatg
     1741 ttgtaaactc caattttgtt agagacctcg aagtatggtt ttacgacaaa aaagaatctt
     1801 caactgaata caacgcttac aaggttttta ccctccgggt acaaattgcc cgcaatgcag
     1861 ccacacctga gcttatcatt tcctatgacg gcatttcgtt ggttcataaa aagagcattc
     1921 ttgacctcga tattgaaaca accatgttta agagggttgt ttacgaaaaa cagatttaca
     1981 aatacgataa actgcccgat gaagcaaagt atcactggga aaacgtctat cctatcctga
     2041 gcaatactct gaaatatgct ttgaatattc cggttcagcc caaaagaaat ggaaccaaca
     2101 ggtacaaagt gtataaatca aacctcgatt atttcattgg aacattcata aaatcaaagc
     2161 aattcagcag tattattgca ttgaatgatg aaaattactt taaagtagaa acaatcagga
     2221 taaagaacac cacacccggc agcaatcgtt tacagttcgg acaagataaa accgatattg
     2281 ttccttatac cggacttatc ggtaatggtc catattctca ttgcccgttt gcccatgtta
     2341 atatgttttt tatttgccac gaaagcgaaa aagacaaagc aaacctgctt tacacctatt
     2401 ttaaagacgg gtataagaac tttcccaaac tcaatcaatt gctcaaagta ccggttaaca
     2461 tcatcaaagc cgaccaaatt acattcacca acaacgaaaa tcctttaccc gaaatcaggc
     2521 aaagtctgtt cagcaaatcc tttgacccca atatccgtta cattgctatt tatttaagcc
     2581 ctttcggtaa aaatcacccc gatacaacca aacggcaata ttactttcag gtaaaagaag
     2641 aattattaaa atacggcata acatcgcagg taattgaaac aacaaaaata tccgatccga
     2701 attacaatta cagtttagtc aatattggcg ttgccattct tgcaaaactc aatggagttc
     2761 cctggcgtt
//
LOCUS       DAOSPF010000141         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_42702_length_2458_cov_5.272992, whole genome shotgun sequence.
ACCESSION   DAOSPF010000141 DAOSPF010000000
VERSION     DAOSPF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42702_length_2458_cov_5.272992"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PK252_14705"
     CDS             <1..105
                     /locus_tag="PK252_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16006.1"
                     /translation="LSRSDRMAKYNQLLRIEEELGDAAIYGYKKVVKK"
     gene            245..1549
                     /locus_tag="PK252_14710"
     CDS             245..1549
                     /locus_tag="PK252_14710"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOT16007.1"
                     /translation="MMKEKLREKIEQWRPRTQRLHDEYGDVVVDKITINQIIGGMRDI
                     KCLVTDISYLDPEEGIKYRNFTLPEVFEKLPRPCGAEYPYVEGCLFLLLTGEIPTEEE
                     VSDVVDELSKRRILPRYVYDVIDAFPDVSHPMAMFASAILTMSRESLFAKKYEMGMRK
                     KDYWDPMYEDSLNLLAKLPEIAAYIYAKLYKNGKMVQSNPFLDFGGNFAHMMDIPKPY
                     DDVSRLHFILHADHESGNVSAHTGHLVASSLSDIYLSISAMICGLAGPLHGLANQEVL
                     KWLHGVIQKMGGKLPTEDEMKQFVWDTLKAGHVIPGFGHAVLRKTDPRYMIQREFALK
                     HMADDPLMHYVDMLFKVVPPILKEQGKAKNPWPNVDSHSGVIQWHYGVREYDFYTVLF
                     GIGRAIGICANLIWDRALLYPLERPKSLTTDMLEVLAMKGGK"
     gene            1914..>2458
                     /locus_tag="PK252_14715"
     CDS             1914..>2458
                     /locus_tag="PK252_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOT16008.1"
                     /translation="MKPKLKLPKAQLVCAVFLFFVTSIFATNIYVKPTGSNTADGLSV
                     ANAKKDISVALLLANAGDTIFVAPGIYRDLIKPQKSGTVSNPITLIADTISKIFTSIA
                     PGPAIIDGADSSFNFNMDISNVSYITIKGFTFQNARIHCIRINSAIADIKGINISDCK
                     FVTTPIMIENPAISINFSSNVG"
BASE COUNT          743 a          414 c          518 g          783 t
ORIGIN      
        1 ttgagtcgtt cagaccgtat ggcaaaatac aaccaactgc ttcgcattga agaagagctt
       61 ggagatgctg ctatttatgg ttataagaag gttgtaaaaa agtaagattt cttactttat
      121 aagataaagc tgcatattgt ataagtatgc agcttttttt gttatttatg caatggtttc
      181 ttgttatttg tttgtaaaat taatagtttt gtaatgttta aaaaatgtat aattattaat
      241 ttttatgatg aaagaaaagc ttcgggaaaa gattgagcaa tggcgcccgc gtacacaaag
      301 gttgcatgat gaatatgggg atgttgtggt agataagatt actatcaacc agataattgg
      361 aggaatgcgg gatataaaat gccttgtgac agacatttca taccttgatc cggaggaagg
      421 aatcaaatat cgtaatttca ctttacctga agtgtttgaa aagctaccaa ggccttgcgg
      481 tgctgaatat ccttatgtag aaggatgtct gttcctttta cttaccggtg aaatacctac
      541 tgaagaagag gtaagcgatg ttgttgatga actttcaaaa cgcaggatat tgcctcgtta
      601 tgtatatgat gttattgatg catttcctga tgtaagccac cctatggcca tgtttgcatc
      661 ggctatcctt actatgtccc gcgaatcgtt atttgccaag aagtatgaaa tgggcatgcg
      721 gaaaaaggat tattgggacc ctatgtacga agattcattg aaccttttgg ccaagctgcc
      781 tgagattgct gcatatatat acgccaagct ttataaaaat ggcaaaatgg tacagtctaa
      841 cccatttctg gattttggag gaaactttgc ccacatgatg gatattccga agccttacga
      901 tgatgtttcg cgtttacatt ttatccttca tgccgaccat gaaagtggaa acgtgagtgc
      961 ccatactggt cacctggtgg ccagttcttt gtcagacata tatttgtcaa tttcagccat
     1021 gatatgtggt ttagcaggcc ctttgcatgg attggccaac caggaagtgc tcaaatggct
     1081 ccatggagtg attcaaaaaa tgggaggtaa gcttcctaca gaagatgaaa tgaaacaatt
     1141 tgtatgggat acgctgaaag ccgggcatgt tattccgggc tttggtcatg ctgtgttgcg
     1201 taaaactgac cctcgctaca tgattcagcg tgagtttgca ttgaagcaca tggccgatga
     1261 cccattgatg cactatgttg acatgttgtt taaagttgtc ccaccaatat taaaggaaca
     1321 agggaaagcc aaaaacccat ggcccaatgt tgactcacat tcgggcgtta tacagtggca
     1381 ttatggtgtg agagaatatg atttctatac agtgcttttt ggtataggaa gggccattgg
     1441 aatttgtgca aacctgattt gggaccgagc tctgctttat ccattggaaa gacccaaatc
     1501 gcttacaact gatatgctgg aagtgctggc gatgaaaggt ggaaaataga gacttctttt
     1561 agaaggggag agttacaatg gttgtttgtt gttttgcctt aattctgcag gtttcaaccc
     1621 ttggcagctt tgttttaaat aagatgcaaa accctaatct gaaagttgat agcttgaaaa
     1681 tataaagcac tgacaggtct ttaagacgct gtctgatcat aatcaatcat atagtccagg
     1741 tctttgactt ggactatatt ttttaatatc aaaagcaaac acgagttaca aactcttgcc
     1801 aagtgtacga caaacgtatc ccaattgctt tttacgttgc tcaaaaaatg ttgctatgac
     1861 gaaaaaaaga gcataacttt aatttctttt tagaataacc taaattaaac catatgaaac
     1921 ccaaacttaa attaccgaaa gcacagctgg tttgtgctgt ttttttattt tttgtaacca
     1981 gtatttttgc tactaacatt tatgttaaac caacaggcag taatacagct gatggtttgt
     2041 ctgttgctaa tgcaaaaaag gatatttcgg ttgctttgct tttagcaaat gccggggaca
     2101 ctatttttgt tgctcccgga atttaccgag acttgattaa gccccagaaa tctggaacag
     2161 tttctaatcc gattacgctt attgctgaca cgataagtaa aatttttaca agtattgcac
     2221 caggaccggc aattattgat ggcgctgatt cgagttttaa ttttaatatg gatatcagca
     2281 atgtttctta tattacaata aaaggtttta cttttcaaaa tgccagaatc cattgtatca
     2341 gaattaattc ggctattgca gatattaaag ggataaatat tagcgattgt aagtttgtta
     2401 caactccaat aatgatagaa aatccggcta tttctataaa ttttagttcg aacgtagg
//
LOCUS       DAOSPF010000142         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_45160_length_2359_cov_8.010417, whole genome shotgun sequence.
ACCESSION   DAOSPF010000142 DAOSPF010000000
VERSION     DAOSPF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45160_length_2359_cov_8.010417"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..>2359
                     /locus_tag="PK252_14720"
     CDS             308..>2359
                     /locus_tag="PK252_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT16009.1"
                     /translation="MQILLIIIFFLLITIVILIIKLQSFRKKTASASLLEETPENLEN
                     LNLEKYTKTLEKLVKEKTFELEQNNQELLTLNEELDAANEELQANNDELNSINEELNN
                     TNTILQHEIEGHKKTQAEKSIVEEKLNKFISQSSEAIVIINEKGIVEDWNDTMTQITG
                     VTKEGALGSFIWDVTYMMAFDPDKAELLRESFKKNTIHFFEEIRKGNSKEQVSETQIR
                     HHDSSHRYIRTTLFPIFTESGYYGGVIISDITQKKAIEAQLETYRNELEMLLEEKNQH
                     LEQLSDRLNEVYTYSSDAITFMDILDEGNTIRVFDLNPVSKKLFKISDEQLLNGVDIT
                     ELLPKIKIDSFRQHILPKLLAGNPVTFTEDRDTGNGYWNSTIYPVKGETGKVHRIAAF
                     SRNVTAEYEKNKTQAILNSAVNSWPYEFWVTDDQGRVILQNKASIEIWGDLSGQPIDV
                     LDIPEETKQFTKEKVQLGLHGEHTSTELVFDTPKGKRYVMFNIHPVFQNDKITGILGM
                     GIDITELKTAEENIRKSEANYRLLAENIEDVIWKMDIETMQYTFITPSIYKLSGFTVE
                     EAMAQSLEDMVSPESLEYAKTNIAERLQQYYQGNLQNITQKNEIELKCKNGSFVWVEV
                     NSTLVADESGKLKEIFASSRNITERKIAEKKIKESEEKFRTIFNSATDGIILLDNNLK
                     IM"
BASE COUNT          883 a          405 c          441 g          630 t
ORIGIN      
        1 tacaacccac cgaaaacggt atgcctttag agctggccgt acacacattt cacaaaccag
       61 cagcagactt tgaagaggta caatcatcta tatttgaata cctgatagcc acagccccac
      121 agtttggctt acgtatcttc caggtaccaa gcggtttcga tgtaaggaaa taaattacaa
      181 caaactacct caaaaacgcc tttgaatgac ctcaaaggcg tttttgttgc tttaacttca
      241 aattaaggtt caattttagc ttatacactt aaaatggatt aactttaaaa tatccaaaaa
      301 tgagtatatg caaattcttc tgattataat tttctttctg cttatcacga ttgtgatatt
      361 gatcattaaa cttcaatctt tcagaaaaaa aacagcttcg gcctctttac ttgaagaaac
      421 acctgaaaat ttagaaaatc tgaatttaga gaaatacact aaaacacttg agaaactggt
      481 caaagagaaa acctttgagt tggaacaaaa caaccaggaa ttacttaccc ttaacgaaga
      541 acttgatgct gctaatgaag aattgcaagc taataacgat gagttaaatt caataaatga
      601 ggaactaaac aatacaaaca caattcttca acatgaaatt gaaggacaca aaaagaccca
      661 ggctgaaaag tcaatagtag aggaaaaact aaacaaattc atttcacagt caagcgaagc
      721 aatagttatt atcaacgaaa aaggcattgt agaagactgg aatgatacaa tgacccaaat
      781 aactggggtt acaaaagaag gagccttagg tagttttatt tgggacgtta cctatatgat
      841 ggcttttgac cctgataagg ccgaattgct tcgggaaagt tttaaaaaaa atacgataca
      901 tttttttgag gaaataagaa agggtaatag taaagagcaa gtttcagaaa ctcaaataag
      961 acatcacgat tcgtctcacc gatatattcg tactacactt tttccaatat ttacggagag
     1021 tggttattat ggaggtgtta taatttcaga tattacccag aaaaaagcta ttgaagccca
     1081 gctcgaaacg taccgaaatg agctggagat gcttttggaa gaaaaaaacc aacatcttga
     1141 acaattgtca gaccgcttaa atgaggtata tacatattct tcagatgcca ttacttttat
     1201 ggatatattg gatgaaggaa acaccataag agtgttcgat ttaaaccctg tatctaagaa
     1261 actttttaaa atatccgatg agcagcttct taatggggta gatatcaccg aattacttcc
     1321 gaaaataaaa attgattctt tcaggcaaca tattttgcct aaactacttg caggaaatcc
     1381 tgtaacattt accgaagata gagataccgg aaatggttat tggaattcaa caatataccc
     1441 tgttaagggg gaaacaggta aagtacatcg cattgcagca ttttcgagaa atgttactgc
     1501 cgaatacgaa aaaaacaaga cgcaagccat tctgaattca gctgtcaaca gttggcctta
     1561 tgagttttgg gttactgatg accaaggacg ggttatcctt caaaataaag cttcaataga
     1621 aatatggggc gatctgtcag gacaacctat tgatgtgctt gatattccgg aagaaactaa
     1681 acaatttact aaagaaaaag tgcagttggg cttacatgga gagcatacaa gcaccgaatt
     1741 ggtttttgac acgccaaagg gcaaacgcta tgtaatgttc aatattcacc cggtgtttca
     1801 aaacgacaag ataacgggga tattgggaat gggtatagat attaccgaat taaaaacagc
     1861 tgaagagaat ataaggaaaa gtgaagcaaa ttacaggctg ttggccgaga atattgaaga
     1921 tgtcatctgg aaaatggaca tcgaaaccat gcaatatact tttataaccc cttcaatata
     1981 taagctctcc ggctttactg ttgaagaagc tatggctcaa agccttgaag atatggtgtc
     2041 acctgaatct ttagaatatg caaaaacaaa tatagctgaa cgattacaac aatattacca
     2101 aggcaatctg caaaacatta ctcaaaaaaa cgaaatagag ctaaaatgta aaaatggcag
     2161 ctttgtatgg gtagaagtaa acagtacttt agtggccgat gagtcgggaa aactcaaaga
     2221 aatatttgcc tcgtcgcgta atattactga gcgcaaaatt gctgaaaaga agatcaaaga
     2281 aagtgaagaa aaatttcgca ctatatttaa ttcggctaca gatggcataa tactgcttga
     2341 caacaacttg aaaataatg
//
LOCUS       DAOSPF010000143         2286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_47130_length_2286_cov_7.376513, whole genome shotgun sequence.
ACCESSION   DAOSPF010000143 DAOSPF010000000
VERSION     DAOSPF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47130_length_2286_cov_7.376513"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1815)
                     /locus_tag="PK252_14725"
     CDS             complement(52..1815)
                     /locus_tag="PK252_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOT16010.1"
                     /translation="MKWKDLKIGYKIGISIAVMILLSGIIGGVALIGMLKIQEDSVNL
                     SSIYIPTINESYSLDKQWHEVIQNLQAYDNSGDEYYLKKAKNRIGKFLASIDILIQVS
                     AENAKLQNSNQDFVHIREQVKQFEAALENYEKLEKQVYLALESFKKVQEIIVQNKGSL
                     PQELINRVNAVSAIAFKASYFRTPADIVKAQNQFINLKTYLNNNKNRGNKEISEALAQ
                     LAEAAGAFAEVYPQAKKLELANFEAYGNIMWNVRGTADIGLDLVKEMGTSTNDTMTQS
                     RYVLIISIVIILIIGTFFALLITRSIVAPVEQSIFMAYQMAEGDLTHVINIKRGDEVG
                     MLTEALNKLNNNLKDIISNIAENADNITQTGIMLSQSANEIAEGARQQASAAEEISSS
                     MEEMYANLQQTTDNAQQTEAISRKSAVEVSKNKESFQVASQSLKQIAEKVNIIDEIAF
                     QTNILALNAAVEAARAGEHGKGFAVVAGEVRKLAEKSKTAAGDINSVSKSTLTLSQSA
                     EKELQALAPEIEKTAKLVQEIAAASIEQVSGIEQINNAMQQLNSVVQGNAQRSDQMTE
                     HSERLSHQADVLRSIITKFKI"
BASE COUNT          711 a          441 c          360 g          774 t
ORIGIN      
        1 aaaaaagcgt gatgtactta caccacgctt cctgctattt gttttgtgtg attagatttt
       61 gaatttagta ataatactcc gcaatacatc ggcctgatgt gataaccttt cagaatgctc
      121 tgtcatttgg tctgagcgtt gggcgttgcc ctggacaacc gagttgagtt gttgcattgc
      181 gttgttaatt tgttcaatac cagaaacctg ctcgatgctg gcagcagcaa tttcctgaac
      241 aagttttgcg gttttttcaa tttcaggggc aagggcctgc aattcttttt cggcagactg
      301 cgaaagggtc aaagttgatt ttgaaacact attaatatct cctgcagcag ttttactctt
      361 ttctgccaac ttacgaacct ctccggcaac aacagcaaaa cctttgccat gttcgccggc
      421 acgagcagct tcaacagcag catttaaagc aagaatattg gtttgaaaag ctatttcatc
      481 aataatatta accttttcag ctatttgctt taacgattga gaagccacct gaaatgattc
      541 tttgttttta gacacctcaa cagcagattt tctcgaaata gcctctgttt gttgggcatt
      601 atctgttgtt tgctgcaagt tggcatacat ttcttccatc gatgaagata tctcctcggc
      661 agcagaagcc tgctgacggg caccctcagc aatttcatta gcgctttgcg aaagcataat
      721 acctgtttgt gtgatattat ctgcattttc tgcaatattg gagataatat cttttaaatt
      781 attgtttagc ttgttcaatg cttcagtaag catacctacc tcgtcgccac gtttaatgtt
      841 gataacatgg gtaaggtccc cttctgccat ttgataagcc ataaaaatac tctgttcaac
      901 cggagcaaca atagaacggg taataagtaa tgcaaagaaa gtcccaatta taagaattat
      961 aacaatagaa attattaaca catacctcga ctgagtcata gtatcattag tactggtacc
     1021 catttcttta accaggtcaa ggcctatatc tgcagtaccc ctgacattcc acatgatatt
     1081 cccgtatgct tcaaaattgg ccagctcaag ttttttagcc tgcggatata cttctgcaaa
     1141 tgccccggca gcttctgcca attgggccaa agcttcgcta atttctttgt tgcccctgtt
     1201 tttgttatta ttaaggtaag ttttaaggtt aataaattga ttttgagctt taacaatatc
     1261 tgcaggtgtc ctgaagtaag aagccttaaa tgcgattgca ctaacagcat tgaccctatt
     1321 tataagttct tgaggaaggc tgcctttgtt ttggacaatt atttcctgta cttttttaaa
     1381 actttctaat gcaagataaa cttgtttttc aagtttttca taattttcta aagcagcctc
     1441 aaattgtttt acctgctcac ggatatgaac gaaatcctgg tttgagtttt gcaactttgc
     1501 attttctgca ctaacctgga ttaaaatatc aatagaagcc aaaaatttgc caatcctatt
     1561 tttagctttc tttaaataat actcgtcacc gctgttatca taggcttgca aattctggat
     1621 tacttcatgc cattgtttgt ctaaagaata tgactcatta atggtaggaa tataaatgct
     1681 tgacaaattt acactatctt cctgaatctt caacatccca atcaaagcaa cccctccaat
     1741 gatgccactt agtagaatca ttacagctat gcttatccca atcttataac ctatttttaa
     1801 atccttccat ttcatagaac ccaagttttg aatctatttt acgaaaataa acaataaaag
     1861 tgataaaaat atcttttttt tttaaattat atgcaattat accttaattg tacaaatgac
     1921 acatatatta tttttattga caatgttaaa aatatgataa atgtcatgaa aatatattgc
     1981 aataactatc aagcaaaata ataattgtca gtctgcaaat tgttataaat attaaaccaa
     2041 tccaataatg ttaaaaacaa attttatagt tttgagtata aacttacacc taaaaactaa
     2101 tattatgaaa ctttcaaaat gcctaatctt taaaattaaa gatcagcggt ttgcagtaaa
     2161 aacccaaaac gttattgatt atatagaaaa agtttacctg attcccaatc aggattcaga
     2221 agaaacctgc catcattact gtcccattaa aatctaaaag agttctttga aaagtttgaa
     2281 atttag
//
LOCUS       DAOSPF010000144         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_66804_length_1776_cov_8.271935, whole genome shotgun sequence.
ACCESSION   DAOSPF010000144 DAOSPF010000000
VERSION     DAOSPF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66804_length_1776_cov_8.271935"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..304)
                     /locus_tag="PK252_14730"
     CDS             complement(44..304)
                     /locus_tag="PK252_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16011.1"
                     /translation="MKQTADNVFLDSNILIYCYTNTELDKQQKVFQVIDNNIDTVRKA
                     NAVKNKYGYSFYDSLIVAAALECGCVTLFSEDMQHGQTIEVV"
     gene            complement(285..494)
                     /locus_tag="PK252_14735"
     CDS             complement(285..494)
                     /locus_tag="PK252_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16012.1"
                     /translation="MIRTVITADKNTLILPLPDNYIGRQLEIIAFAIDEPVKQTKRAS
                     AKDVFKALKFDTRGFKFNRDEANSR"
     gene            complement(506..670)
                     /locus_tag="PK252_14740"
     CDS             complement(506..670)
                     /locus_tag="PK252_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16013.1"
                     /translation="MPKEVTVEEKTTLEQVRLIQQLDDEDKHVIFKMNDTMLTKKKFK
                     DFFQKNVALL"
     gene            1194..>1776
                     /locus_tag="PK252_14745"
     CDS             1194..>1776
                     /locus_tag="PK252_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT16014.1"
                     /translation="MGQKYMHTAPHTAMPAMQPRFDCGHTGHEMLCGFGLNTPLRYAD
                     PSGWQNQPVITLPGGLSFYTLEGYHAYLRSLGHETPAFAGSYRYGPFQNGFGPGQFSG
                     SGSLYAYDDYSTYDVVSNTFGNERWNGSFAGQVQSAYSNQQPNTYAQYEVNNDGSLNK
                     TFETSMDNIQIVSSQNNINIYYTPLIAGNGPSGG"
BASE COUNT          495 a          385 c          332 g          564 t
ORIGIN      
        1 cagcattatc tggttacttt tatgagtgtt tacacaatct tttttatact acctctatgg
       61 tttgaccatg ctgcatgtct tcggagaata gtgtaacaca accacattct aaggccgcag
      121 caacaataag cgaatcataa aatgaataac catatttgtt tttaacagca ttggcttttc
      181 ttacagtatc aatattgttg tctataacct gaaaaacttt ttgttgttta tccagttcgg
      241 tattggtata gcaataaatc agtatattac tgtcaaggaa aacattatcg gctgtttgct
      301 tcatcacggt taaatttaaa cccacgggta tcgaatttta aagccttaaa cacgtcttta
      361 gctgaagccc ttttggtctg ctttacaggc tcatctattg caaacgcaat aatctcaagt
      421 tgtctgccta tatagttatc aggcaaaggc aaaatgagcg tgtttttatc tgcggtaatt
      481 actgttcgta tcatatcctt aatttttata ataatgcaac gttcttttga aaaaaatctt
      541 tgaatttctt tttggtaagc atggtatcat tcattttaaa aataacatgc ttgtcctcgt
      601 cgtccaattg ttgaattaac cgaacctgtt caagggttgt tttctcttct acagttactt
      661 cctttggcat ccctttattt gggtttaaaa tatcgtcaac tgtaacaccg aaaagacctg
      721 aaactcgtta agcacttcaa cagaaagttc acgcttgtca tattcaatct tgttataatt
      781 agactggtca atacaagttc aattgcaact tgcttttgct gtaatccctt ttcctttatg
      841 ttgtcgccta cgttcataac tgttttgttt tgcatcaaaa ttaggcaaaa tgaccattaa
      901 taacaaatag ttgataaaaa aggaaatctc aaattggttg ttatggaaat ttaataccca
      961 gttagcgcac gtttgtaact ggggtgttga attttatatt atttagctaa aatatattca
     1021 tttccccgtt catcgtagct taccaataaa agcggtccgg cgtagcatcc tgctacgtcg
     1081 cattgtattc ggcctccggc cgtattacat cttaaggctg cagcctgtag ccaagagtag
     1141 ccaatacagg ccaaagcctt gaccaggtgc aataaaatgc ctatctttgg tacatgggcc
     1201 aaaagtacat gcatactgcc ccgcacaccg ccatgccggc catgcagccc cgcttcgact
     1261 gcgggcacac cggccacgag atgctatgcg gctttggcct caacaccccc ctgcgctacg
     1321 ccgacccttc gggctggcaa aaccaacctg taataacgct gcccggcggg ctgtcatttt
     1381 acacacttga aggttatcat gcttatttgc gcagtttggg ccatgaaacg cctgcatttg
     1441 ctggcagcta ccgttacggc ccttttcaaa acggctttgg gccgggccaa ttttctggct
     1501 cggggagcct ttatgcttat gacgattata gcacgtatga tgtggtgagc aatacttttg
     1561 ggaatgaaag atggaatggg tcttttgcag gtcaggtaca atccgcatac agcaaccaac
     1621 aaccaaatac ctatgcgcaa tatgaagtta ataatgatgg ttctttaaat aaaacattcg
     1681 agacttcaat ggacaacatc caaatagtat catcccaaaa taacattaat atatactata
     1741 cccctttgat tgctggtaat gggccttctg gcggtt
//
LOCUS       DAOSPF010000145         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2326
            NODE_79358_length_1570_cov_4.360396, whole genome shotgun sequence.
ACCESSION   DAOSPF010000145 DAOSPF010000000
VERSION     DAOSPF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864393
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:33:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,950
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 38
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 12 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79358_length_1570_cov_4.360396"
                     /isolate="AMR_MDS_2326"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..>1570)
                     /locus_tag="PK252_14750"
     CDS             complement(618..>1570)
                     /locus_tag="PK252_14750"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT16015.1"
                     /translation="DMCLEVINSKEYELVGANGETFNTFWSPTTNGFRYLFSVEGNNN
                     KESIFAVQHIHSTGYGNYSFGCALNQFVGPRALIRKDGSFPTQGDHAWGFWVPTHKLY
                     NLFDPNDVRRKVAIGQGPDPETGYPGDSVYGQVTVNGKKVTGFFVIANTTYQATGLEN
                     MKYEIGPHNSMIIDGGFQGNTQNMYYIRYADVILLAAEAAMMLDDQANASKYFNMIRA
                     RARNCGDGIHPADLAGNVTKQDIMDERAREFAMEGERFFDLVRWKEAYNNINGSTMEW
                     WKNNPSYNGITITYHDKNDFYPIPAIETSKNNNLKQYQGW"
BASE COUNT          464 a          308 c          299 g          499 t
ORIGIN      
        1 ttgccctcgc ttcgccgata aagctttttt cttaacgcca tttccgtcag gccaatagct
       61 gaaagtttca atgaaatagg cgaagcatta aaaagaaaaa ttccttatca tcgccttttg
      121 ttcgatttcg gccgagaagc ctaacgttcc tgttgtttgc atattgcaat attcacgggc
      181 ttcgagggtg aaagagttaa ggcgataaga gttttgagca acttttttcg ataaaaaagt
      241 tgcatagaga cttagtattt attgctattc aatgcttctt aaacaataaa tagccttttc
      301 tttaaagctt ttctataaat aaaaagcgga atcagtcagg ctccgctttt atttttgggg
      361 tagtagcaaa aaagcaaaat taagcatgaa aagtgcttaa tcggttaaaa aattgtaatt
      421 cagtatttta atagaaattt tcaaacaatt tctatattag cttgtagcgt ttatgacgta
      481 agggataacc caactaaatc atataattcg gcatagtatt ttctcataat ggttaagttt
      541 tatcaaactc aggttaattg taaaaaaaag ccctaaaaag gataatcttt tcagggcttg
      601 taaaaaaaga ctaacaatta ccaaccttga tattgcttca aattgttgtt ttttgaagtt
      661 tcaatggcag gtattgggta aaaatcattt ttatcatgat aggtaatagt tataccatta
      721 taacttgggt tgtttttcca ccattccata gtagaaccat tgatgttgtt ataagcttct
      781 ttccaacgga ccaggtcgaa aaaccgttct ccttccatag caaattcgcg ggcgcgttca
      841 tccattatat cttgtttagt aacgtttccg gccaaatctg ccggatgtat gccgtctccg
      901 caatttctgg cccgggcgcg gatcatgtta aaatatttgg aagcattagc ctggtcgtct
      961 aacatcattg cagcctcggc agctaacaat atcacatcgg cataacggat ataatacatg
     1021 ttttgggtat tgccctgaaa gcctccatca ataatcatag agttgtgcgg gcctatttca
     1081 tacttcatgt tttcaagtcc tgtagcctga taggtagtat tggcaatgac aaaaaagcct
     1141 gtaacctttt tcccgttaac agtgacttgt ccatacacac tatcgccagg atatccggtt
     1201 tcagggtcag ggccttgacc tatggctacc ttgcgcctta cgtcattagg gtcaaacaaa
     1261 ttgtacagtt tgtgagttgg tacccagaaa ccccatgcgt ggtcgccctg agtaggaaat
     1321 gagccatctt tgcggattaa tgcacgaggc ccgacaaatt ggtttaatgc acagccaaaa
     1381 ctataatttc cgtaaccagt agagtggata tgctgaacag caaaaatact ttccttattg
     1441 ttgttgcctt ctaccgaaaa tagataacgg aacccgtttg ttgttgggct ccaaaatgta
     1501 ttgaaagttt cgccatttgc cccaacaagt tcatattctt ttgaattgat aacctccagg
     1561 cacatatcgt
//
